FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Gorell, JM Johnson, CC Rybicki, BA Peterson, EL Kortsha, GX Brown, GG Richardson, RJ AF Gorell, JM Johnson, CC Rybicki, BA Peterson, EL Kortsha, GX Brown, GG Richardson, RJ TI Occupational exposure to manganese, copper, lead, iron, mercury and zinc and the risk of Parkinson's disease SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 15th International Neurotoxicology Conference CY OCT 26-29, 1997 CL LITTLE ROCK, ARKANSAS SP Agcy Tox Substances & Dis Registry, US EPA, Natl Human & Environm Effects Res Lab, Natl Ctr Environm Assessment, Ferroalloys Assoc, Int Manganese Inst, Natl Inst Environm Hlth Sci, Ethyl Corp, NINDS, Ctr Dis Control & Prevent DE manganese; copper; iron; lead; mercury; zinc; Parkinson's disease ID SUBSTANTIA-NIGRA; TRANSITION-METALS; DNA-DAMAGE; BRAIN; CATECHOLAMINES; INCREASE AB A population-based case-control study was conducted in the Henry Ford Health System (HFHS) in metropolitan Detroit to assess occupational exposures to manganese, copper, lead, iron, mercury and zinc as risk factors for Parkinson's disease (PD). Non-demented men and women 50 years of age who were receiving primary medical care at HFHS were recruited, and concurrently enrolled cases (n = 744) and controls (n = 464) were frequency-matched for sex, race and age (+/- 5 years). A risk factor questionnaire, administered by trained interviewers, inquired about every job held by each subject for 6 months from age 18 onward, including a detailed assessment of actual job tasks, tools and environment. An experienced industrial hygienist, blinded to subjects' case-control status, used these data to rate every job as exposed or not exposed to one or more of the metals of interest. Adjusting for sex, race, age and smoking status, 20 years of occupational exposure to any metal was not associated with PD. However, more than 20 years exposure to manganese (Odds Ratio [OR] = 10.61, 95% Confidence Interval [CI] = 1.06, 105.83) or copper (OR = 2.49, 95% CI = 1.06,5.89) was associated with PD. Occupational exposure for > 20 years to combinations of lead-copper (OR = 5.24, 95% CI = 1.59,17.21), lead-iron (OR = 2.83, 95% CI = 1.07,7.50), and iron-copper (OR = 3.69, 95% CI = 7.40, 9.71) was also associated with the disease. No association of occupational exposure to iron, mercury or zinc with PD was found. A lack of statistical power precluded analyses of metal combinations for chose with a low prevalence of exposure (i.e., manganese, mercury and zinc). Our findings suggest that chronic occupational exposure to manganese or copper, individually, or to dual combinations of lead iron and copper, is associated with PD. (C) 1999 Infer Press, Inc. C1 Henry Ford Hlth Syst, Dept Neurol, Detroit, MI USA. Henry Ford Hlth Syst, Dept Biostat & Res Epidemiol, Detroit, MI USA. Vet Adm Med Ctr, Psychol Serv, San Diego, CA 92161 USA. Univ Calif San Diego, Dept Psychiat, San Diego, CA 92103 USA. Univ Michigan, Sch Publ Hlth, Dept Environm & Ind Hlth, Toxicol Program, Ann Arbor, MI 48109 USA. Wayne State Univ, NIEHS, Ctr Mol & Cellular Toxicol Human Applicat, Detroit, MI USA. RP Gorell, JM (reprint author), Henry Ford Heart & Hlth Sci Ctr, Dept Neurol, 2799 W Grand Blvd, Detroit, MI 48202 USA. OI Richardson, Rudy/0000-0002-2028-5723; Johnson, Christine Cole/0000-0002-6864-6604 FU NIEHS NIH HHS [ES 06418]; NINDS NIH HHS [NS 30618] NR 32 TC 258 Z9 265 U1 1 U2 32 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 USA SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD APR-JUN PY 1999 VL 20 IS 2-3 BP 239 EP 247 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA 205ZE UT WOS:000080850400011 PM 10385887 ER PT J AU Sziraki, I Rauhala, P Koh, KK van Bergen, P Chiueh, CC AF Sziraki, I Rauhala, P Koh, KK van Bergen, P Chiueh, CC TI Implications for atypical antioxidative properties of manganese in iron-induced brain lipid peroxidation and copper-dependent low density lipoprotein conjugation SO NEUROTOXICOLOGY LA English DT Article; Proceedings Paper CT 15th International Neurotoxicology Conference CY OCT 26-29, 1997 CL LITTLE ROCK, ARKANSAS SP Agcy Tox Substances & Dis Registry, US EPA, Natl Human & Environm Effects Res Lab, Natl Ctr Environm Assessment, Ferroalloys Assoc, Int Manganese Inst, Natl Inst Environm Hlth Sci, Ethyl Corp, NINDS, Ctr Dis Control & Prevent DE Fenton reaction; hydroxyl radical; lipid peroxidation; oxidative stress; transition metals ID OXIDATIVE STRESS; ALZHEIMERS-DISEASE; PARKINSONS-DISEASE; IN-VIVO; HYDROXYL RADICALS; DOPAMINE NEURONS; RAT-BRAIN; NIGROSTRIATAL NEURONS; GLUTAMINE-SYNTHETASE; SODIUM-NITROPRUSSIDE AB Our group recently observed that manganese prevents oxidative brain injury in the iron-induced parkinsonian animal model. It has also been suggested that manganese retards while copper promotes the development of atherosclerosis. In this report, we provide further evidence to support a controversial notion that manganese is an atypical antioxidant. Among transition metals, Cu2+ and Fe2+ (0.1 to 125 mu M) but not Mn2+, converted hydrogen peroxide to reactive hydroxyl radicals via the Fenton reaction at pH 7.4. Iron's pro-oxidative rate is relatively slow, but it is accelerated further by ascorbate (50 mu M) in 37 degrees C Dulbecco's phosphate buffered saline. Moreover, Mn2+ (0-80 mu M) concentration dependently retarded diene conjugation of human low density lipoproteins stimulated by 5 mu M Cu2+. This new result is consistent with our recent finding that Mn2+ (0 to 20 mu M) does not initiate bra in lipid peroxidation while it inhibits iron-induced peroxidation of polyunsaturated fatty acids. These unexpected manganese results are somewhat at odds with a prominent theory that manganese is a prooxidative transition metal. Furthermore, iron and copper induced free radical generation and lipid peroxidation are suppressed by lowering the incubation temperature; this suggests that hypothermia may decrease the oxidative stress and damage in vivo. In conclusion, normal dietary intake of manganese may protect cells and neurons from oxidant stress through the inhibition of propagation of lipid peroxidation caused by hydroxyl radicals generated by prooxidative transition metals such as iron and copper. Potential therapeutical uses of manganese, manganese SOD mimetics and hypothermia for protecting brain neurons and vascular endothelial cells aga inst oxidative stress and damage have been successfully demonstrated in both animal models and clinical trials. (C) 1999 Inter Press, Inc. C1 NIMH, Unit Neurodegenerat & Neuroprotect, Clin Sci Lab, NIH 10 3D41, Bethesda, MD 20892 USA. NHLBI, Cardiol Branch, Ctr Clin, NIH, Bethesda, MD 20892 USA. RP Chiueh, CC (reprint author), NIMH, Unit Neurodegenerat & Neuroprotect, Clin Sci Lab, NIH 10 3D41, Bethesda, MD 20892 USA. OI Rauhala, Pekka/0000-0003-2036-3522 NR 84 TC 28 Z9 28 U1 0 U2 2 PU INTOX PRESS INC PI LITTLE ROCK PA PO BOX 24865, LITTLE ROCK, AR 72221 USA SN 0161-813X J9 NEUROTOXICOLOGY JI Neurotoxicology PD APR-JUN PY 1999 VL 20 IS 2-3 BP 455 EP 466 PG 12 WC Neurosciences; Pharmacology & Pharmacy; Toxicology SC Neurosciences & Neurology; Pharmacology & Pharmacy; Toxicology GA 205ZE UT WOS:000080850400028 PM 10385904 ER PT J AU Andiman, W Boucher, M Burns, D Bryson, Y Farley, J Fowler, H Gabiano, C Galli, L Hutto, C Kind, C Korber, B Kovacs, A Krogstad, P Landesman, S Lapointe, N Lemay, M Lew, J Mandelbrot, L Mayaux, MJ Mellins, R Minkoff, H Mofenson, L Nielsen, K Newell, ML Pardi, G Peavy, H Peckham, C Read, J Rother, C Rudin, C Scott, G Semprini, A Shearer, W Simonds, R Simpson, B Stek, A Tovo, PA Tuomala, R Van Dyke, R Weedon, J de Martino, M Lindsay, M Belair, S Chan, L Harris, D Kalish, L Muenz, L Nugent, R Schluchter, M Durako, S Goodwin, S Mitchell, R Nourjah, P Owen, W Widmayer, S Bardeguez, A Hanson, C Wiznia, A Luzuriaga, K Viscarello, R Ho, D Koup, R Chen, I Mullins, J Wolinsky, S Walker, B Ammann, A Clapp, S McDonald, D Fauvel, M Hankins, C Samson, J Bailey, A Giaquinto, C Ruga, E De Rossi, A Truscia, D Grosch-Worner, I Schafer, A Mok, J Johnstone, F Jiminez, J de Alba, C Garcia-Rodriguez, M Bates, I de Jose, I Hawkins, F Zapico, RM Asensi-Botet, F Otero, M Perez-Tamarit, D Moya, A Galbis, M Scherpbier, H Boer, K Bohlin, A Lindgren, S Ehrnst, A Anzen, B Belfrage, E Levy, J Alimenti, A Barlow, P Ferrazin, A De Maria, A Gotta, C Maritati, V Mur, A Rovira, M Paya, A Coll, O Fortuny, C Boguna, J Caro, MC Canet, Y Pardi, G Ravizza, M Castagna, C Fiore, S Guerra, B Lanari, M Bianchi, S Bovicelli, L Prati, E Duse, M Soresina, A Scaravelli, G De Santis, M Muggiasca, M Vigano, A Marchisio, P Iasci, A Spinillo, A Bucceri, A Grossi, E Rancilio, L Della Torre, M Dallacasa, P Soresina, A Pachi, A Principi, N Muggiasca, M Marchisio, P Zara, C Vignali, M Rossi, G Rancilio, L Selvaggi, L Greco, P Vimercati, A Massi, G Innocenti, T Fiscella, A Sansone, M Benedetto, C Tibaldi, C Ziarati, N Tadrist, B Thevenicau, D Gondry, J Paulard, B Alisy, C Brault, D Tordjeman, P Mamou, J Rozan, M Colombani, D Pincemaille, O Salvetti, A Chabanier, C Hernandorena, X Leroy, J Schaal, J Balde, P Faucher, P Lachassinne, E Benoit, S Douard, D Hocke, C Barjot, P Brouard, J Delattre, P Stien, L Audibert, F Labrune, P Vial, M Mazy, F Sitbon, D Crenn-Hebert, C Floch-Tudal, C Akakpo, R Daveau, C Leblanc, A Cesbron, P Duval-Arnould, H Huraux-Rendu, C Lemerle, S Touboul, C Guerin, M Maingueneau, C Reynaud, I Rousseau, T Ercoil, V Lanza, M Denavit, M Garnier, J Lahsinat, K Pia, R Allouche, C Nardou, M Grall, F May, A Dallot, M Lhuillier, P Cecile, W Mezin, R Balde, P Bech, A Lobut, J Algava, G Dermesay, AC Busuttil, R Jacquemot, M Bader-Meunier, B Fridman, S Codaccioni, X Maxingue, F Thomas, D Alain, J De Lumley, L Tabaste, J Salin, PB Seaume, H Guichard, A Kebaili, K Roussouly, C Botto, C De Lanete, A Wipff, P Cravello, L De Boisse, P Leclaire, M Michel, G Crumiere, C Lefevre, V Le Lorier, B Pauly, I Robichez, B Seguy, D Dehlinger, M Rideau, F Talon, P Benos, P Huret, C Nicolas, J Heller-Roussin, B Saint-Leger, S Delaporte, M Hubert, C De Sarcus, B Karoubi, P Mechinaud, F Bertcrottiere, D Bongain, A Monpoux, F De Gennes, C Devianne, F Nisand, I Rousset, M Karoubi, P Mouchnino, G Muray, J Munzer, M Quereux, C Brossard, V Clavier, B Allemon, M Rotten, D Stephan, J Varlet, M Guyot, B Narey, P Bardinet, F De Caunes, F Jeny, R Robin, M Bouley, AR Savey, L Berrebi, A Tricoire, J Borderon, J Fignon, A Guillot, F Maria, B Broyard, A Chitrit, Y Firtion, G Mandelbrot, L Pillet, ML Parat, S Boissinot, C Garec, N Levine, M Ottenwalter, A Schaller, F Vilmer, B Courpotin, C Brunner, C Ciraru-Vigneron, N Hatem-Gantzer, G Heller-Roussin, B Fritel, X Wallet, A Bouille, J Milliez, J Mrejen, DB Dermer, E Noseda, G Bardou, D Cressaty, J Francoual, C Moncomble, CC Cohen, H Blanche, S Bastion, H Benifla, J Benkhatar, F Berkane, N Herve, F Ronzier, M Mayaux, MJ de Martino, M Tovo, PA Galli, L Gabiano, C Ferraris, G Rancillo, L Bucceri, A Tulisso, S Scolfaro, C Riva, C Vierucci, A de Luca, M Farina, S Fundaro, C Genovese, O Mercu, G Forni, G Stegagno, M Falconieri, P Zuccotti, G Riva, E Cellini, M Baraldi, C Consolini, R Palla, G Ruggeri, M Pignata, C Guarino, A Osimani, P Metri, A Antonellini, A Benaglia, G Romano, A Dallacasa, P De Mattia, D Caselli, D Boni, S Dell'Erba, G Bassanetti, F Sticca, M Timpano, C Magnani, C Salvatore, C Gambaretto, G Lipreri, R Tornaghi, R Pinzani, R Cecchi, M Bezzi, T Battisti, L Bresciani, E Gattinara, G Berrino, R Pellegatta, A Mazza, A Baldi, F Micheletti, E Ruga, E Altobelli, R Deiana, M Colnaghi, C Tarallo, L Tondo, U Anastasio, E Duse, M Chiriaco, P Contardi, I Ruggeri, C Ibba, P Scott, G Hutto, C O'Sullivan, M Malmsberry, A Willoughby, A Burns, D Goedert, J Landesman, S Minkoff, H Mendez, H Holman, S Rubinstein, A Durako, S Muenz, L Goodwin, S Nesheim, S Lindsay, M Clark, S Lee, F Nahmias, A Sawyer, M Vink, P Farley, J Alger, L Abrams, E Bamji, M Lambert, G Schoenbaum, E Thea, D Thomas, P Weedon, J Palumbo, P Bardeguez, A Denny, T Oleske, J Simonds, R Orloff, S Ethier-Ives, J Rogers, M Schluchter, M Kutner, M Kaplan, S Kattan, M Lipshultz, S Mellins, R Shearer, W Peavy, H Sopko, G Sloand, E Wu, M Kind, C Nadal, D Rudin, C Siegrist, CA Wyler, CA Cheseaux, JJ Aebi, C Gnehm, H Schubiger, G Klingler, J Hunziker, U Kuchler, H Gianinazzi, M Buhlmann, U Rudin, C Biedermann, K Lauper, U Irion, O Brunelli, A Spoletini, G Schreyer, A Kind, C Hosli, I Saurenmann, E Drack, G Isenschmid, M Poorbeik, M Schupbach, J Perrin, L Erb, P Joller, H Bryson, Y Dillon, M Nielsen, R Boyer, P Liao, D Keller, M Deveikis, A Kovacs, A Stek, A Chan, L Rother, C Khoury, M Diaz, C Pacheco-Acosta, E Tuomala, R Cooper, E Mesthene, D Pitt, J Higgins, A Mendez, H Moroso, G Rich, K Turpin, D Cooper, N Fowler, M Nugent, R Smeriglio, V McKinlay, S Kalish, L Ellis, S Andiman, W Simpson, B AF Andiman, W Boucher, M Burns, D Bryson, Y Farley, J Fowler, H Gabiano, C Galli, L Hutto, C Kind, C Korber, B Kovacs, A Krogstad, P Landesman, S Lapointe, N Lemay, M Lew, J Mandelbrot, L Mayaux, MJ Mellins, R Minkoff, H Mofenson, L Nielsen, K Newell, ML Pardi, G Peavy, H Peckham, C Read, J Rother, C Rudin, C Scott, G Semprini, A Shearer, W Simonds, R Simpson, B Stek, A Tovo, PA Tuomala, R Van Dyke, R Weedon, J de Martino, M Lindsay, M Belair, S Chan, L Harris, D Kalish, L Muenz, L Nugent, R Schluchter, M Durako, S Goodwin, S Mitchell, R Nourjah, P Owen, W Widmayer, S Bardeguez, A Hanson, C Wiznia, A Luzuriaga, K Viscarello, R Ho, D Koup, R Chen, I Mullins, J Wolinsky, S Walker, B Ammann, A Clapp, S McDonald, D Fauvel, M Hankins, C Samson, J Bailey, A Giaquinto, C Ruga, E De Rossi, A Truscia, D Grosch-Worner, I Schafer, A Mok, J Johnstone, F Jiminez, J de Alba, C Garcia-Rodriguez, M Bates, I de Jose, I Hawkins, F Zapico, RM Asensi-Botet, F Otero, M Perez-Tamarit, D Moya, A Galbis, M Scherpbier, H Boer, K Bohlin, A Lindgren, S Ehrnst, A Anzen, B Belfrage, E Levy, J Alimenti, A Barlow, P Ferrazin, A De Maria, A Gotta, C Maritati, V Mur, A Rovira, M Paya, A Coll, O Fortuny, C Boguna, J Caro, MC Canet, Y Pardi, G Ravizza, M Castagna, C Fiore, S Guerra, B Lanari, M Bianchi, S Bovicelli, L Prati, E Duse, M Soresina, A Scaravelli, G De Santis, M Muggiasca, M Vigano, A Marchisio, P Iasci, A Spinillo, A Bucceri, A Grossi, E Rancilio, L Della Torre, M Dallacasa, P Soresina, A Pachi, A Principi, N Muggiasca, M Marchisio, P Zara, C Vignali, M Rossi, G Rancilio, L Selvaggi, L Greco, P Vimercati, A Massi, G Innocenti, T Fiscella, A Sansone, M Benedetto, C Tibaldi, C Ziarati, N Tadrist, B Thevenicau, D Gondry, J Paulard, B Alisy, C Brault, D Tordjeman, P Mamou, J Rozan, M Colombani, D Pincemaille, O Salvetti, A Chabanier, C Hernandorena, X Leroy, J Schaal, J Balde, P Faucher, P Lachassinne, E Benoit, S Douard, D Hocke, C Barjot, P Brouard, J Delattre, P Stien, L Audibert, F Labrune, P Vial, M Mazy, F Sitbon, D Crenn-Hebert, C Floch-Tudal, C Akakpo, R Daveau, C Leblanc, A Cesbron, P Duval-Arnould, H Huraux-Rendu, C Lemerle, S Touboul, C Guerin, M Maingueneau, C Reynaud, I Rousseau, T Ercoil, V Lanza, M Denavit, M Garnier, J Lahsinat, K Pia, R Allouche, C Nardou, M Grall, F May, A Dallot, M Lhuillier, P Cecile, W Mezin, R Balde, P Bech, A Lobut, J Algava, G Dermesay, AC Busuttil, R Jacquemot, M Bader-Meunier, B Fridman, S Codaccioni, X Maxingue, F Thomas, D Alain, J De Lumley, L Tabaste, J Salin, PB Seaume, H Guichard, A Kebaili, K Roussouly, C Botto, C De Lanete, A Wipff, P Cravello, L De Boisse, P Leclaire, M Michel, G Crumiere, C Lefevre, V Le Lorier, B Pauly, I Robichez, B Seguy, D Dehlinger, M Rideau, F Talon, P Benos, P Huret, C Nicolas, J Heller-Roussin, B Saint-Leger, S Delaporte, M Hubert, C De Sarcus, B Karoubi, P Mechinaud, F Bertcrottiere, D Bongain, A Monpoux, F De Gennes, C Devianne, F Nisand, I Rousset, M Karoubi, P Mouchnino, G Muray, J Munzer, M Quereux, C Brossard, V Clavier, B Allemon, M Rotten, D Stephan, J Varlet, M Guyot, B Narey, P Bardinet, F De Caunes, F Jeny, R Robin, M Bouley, AR Savey, L Berrebi, A Tricoire, J Borderon, J Fignon, A Guillot, F Maria, B Broyard, A Chitrit, Y Firtion, G Mandelbrot, L Pillet, ML Parat, S Boissinot, C Garec, N Levine, M Ottenwalter, A Schaller, F Vilmer, B Courpotin, C Brunner, C Ciraru-Vigneron, N Hatem-Gantzer, G Heller-Roussin, B Fritel, X Wallet, A Bouille, J Milliez, J Mrejen, DB Dermer, E Noseda, G Bardou, D Cressaty, J Francoual, C Moncomble, CC Cohen, H Blanche, S Bastion, H Benifla, J Benkhatar, F Berkane, N Herve, F Ronzier, M Mayaux, MJ de Martino, M Tovo, PA Galli, L Gabiano, C Ferraris, G Rancillo, L Bucceri, A Tulisso, S Scolfaro, C Riva, C Vierucci, A de Luca, M Farina, S Fundaro, C Genovese, O Mercu, G Forni, G Stegagno, M Falconieri, P Zuccotti, G Riva, E Cellini, M Baraldi, C Consolini, R Palla, G Ruggeri, M Pignata, C Guarino, A Osimani, P Metri, A Antonellini, A Benaglia, G Romano, A Dallacasa, P De Mattia, D Caselli, D Boni, S Dell'Erba, G Bassanetti, F Sticca, M Timpano, C Magnani, C Salvatore, C Gambaretto, G Lipreri, R Tornaghi, R Pinzani, R Cecchi, M Bezzi, T Battisti, L Bresciani, E Gattinara, G Berrino, R Pellegatta, A Mazza, A Baldi, F Micheletti, E Ruga, E Altobelli, R Deiana, M Colnaghi, C Tarallo, L Tondo, U Anastasio, E Duse, M Chiriaco, P Contardi, I Ruggeri, C Ibba, P Scott, G Hutto, C O'Sullivan, M Malmsberry, A Willoughby, A Burns, D Goedert, J Landesman, S Minkoff, H Mendez, H Holman, S Rubinstein, A Durako, S Muenz, L Goodwin, S Nesheim, S Lindsay, M Clark, S Lee, F Nahmias, A Sawyer, M Vink, P Farley, J Alger, L Abrams, E Bamji, M Lambert, G Schoenbaum, E Thea, D Thomas, P Weedon, J Palumbo, P Bardeguez, A Denny, T Oleske, J Simonds, R Orloff, S Ethier-Ives, J Rogers, M Schluchter, M Kutner, M Kaplan, S Kattan, M Lipshultz, S Mellins, R Shearer, W Peavy, H Sopko, G Sloand, E Wu, M Kind, C Nadal, D Rudin, C Siegrist, CA Wyler, CA Cheseaux, JJ Aebi, C Gnehm, H Schubiger, G Klingler, J Hunziker, U Kuchler, H Gianinazzi, M Buhlmann, U Rudin, C Biedermann, K Lauper, U Irion, O Brunelli, A Spoletini, G Schreyer, A Kind, C Hosli, I Saurenmann, E Drack, G Isenschmid, M Poorbeik, M Schupbach, J Perrin, L Erb, P Joller, H Bryson, Y Dillon, M Nielsen, R Boyer, P Liao, D Keller, M Deveikis, A Kovacs, A Stek, A Chan, L Rother, C Khoury, M Diaz, C Pacheco-Acosta, E Tuomala, R Cooper, E Mesthene, D Pitt, J Higgins, A Mendez, H Moroso, G Rich, K Turpin, D Cooper, N Fowler, M Nugent, R Smeriglio, V McKinlay, S Kalish, L Ellis, S Andiman, W Simpson, B CA Int Perinatal HIV Grp TI The mode of delivery and the risk of vertical transmission of human immunodeficiency virus type 1 - A meta-analysis of 15 prospective cohort studies SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Article; Proceedings Paper CT 12th International Conference on AIDS CY JUN, 1998 CL GENEVA, SWITZERLAND ID MOTHER-TO-CHILD; INFANT HIV TRANSMISSION; CESAREAN-SECTION; ZIDOVUDINE PROPHYLAXIS; OBSTETRIC FACTORS; INFECTION; WOMEN; COMPLICATIONS; PREVENTION; REDUCTION AB Background To evaluate the relation between elective cesarean section and vertical transmission of human immunodeficiency virus type 1 (HIV-1), we performed a meta-analysis using data on individual patients from 15 prospective cohort studies. Methods North American and European studies of at least 100 mother-child pairs were included in the meta-analysis. Uniform definitions of modes of delivery were used. Elective cesarean sections were defined as those performed before onset of labor and rupture of membranes. Multivariate logistic-regression analysis was used to adjust for other factors known to be associated with vertical transmission. Results The primary analysis included data on 8533 mother-child pairs. After adjustment for receipt of antiretroviral therapy, maternal stage of disease, and infant birth weight, the likelihood of vertical transmission of HIV-1 was decreased by approximately 50 percent with elective cesarean section, as compared with other modes of delivery (adjusted odds ratio, 0.43; 95 percent confidence interval, 0.33 to 0.56). The results were similar when the study population was limited to those with rupture of membranes shortly before delivery. The likelihood of transmission was reduced by approximately 87 percent with both elective cesarean section and receipt of antiretroviral therapy during the prenatal, intrapartum, and neonatal periods, as compared with other modes of delivery and the absence of therapy (adjusted odds ratio, 0.13; 95 percent confidence interval, 0.09 to 0.19), Among mother-child pairs receiving antiretroviral therapy during the prenatal, intrapartum, and neonatal periods, rates of vertical transmission were 2.0 percent among the 196 mothers who underwent elective cesarean section and 7.3 percent among the 1255 mothers with other modes of delivery. Conclusions The results of this meta-analysis suggest that elective cesarean section reduces the risk of transmission of HIV-1 from mother to child independently of the effects of treatment with zidovudine. (N Engl J Med 1999;340:977-87.) (C)1999, Massachusetts Medical Society. C1 NICHHD, Pediat Adolescent & Maternal AIDS Branch, NIH, Bethesda, MD 20892 USA. RP Read, J (reprint author), NICHHD, Pediat Adolescent & Maternal AIDS Branch, NIH, Execut Bldg,Rm 4B11F,6100 Execut Blvd,MSC 7510, Bethesda, MD 20892 USA. RI Manzotti, Grazia/C-5985-2008; Wolinsky, Steven/B-2893-2012; SHCS, MoCHIV/G-4081-2011; vimercati, antonella/I-8114-2012; peckham, catherine/I-6300-2013; Pignata, Claudio/O-2466-2013; SHCS, int. coll. B/G-4090-2011; SHCS, all/G-4072-2011; SHCS, ch/G-4077-2011; De Rossi, Anita/L-3128-2015; de maria, andrea/F-7116-2016 OI vimercati, antonella/0000-0002-9862-1619; Pignata, Claudio/0000-0003-1568-9843; De Rossi, Anita/0000-0001-6435-7509; de maria, andrea/0000-0001-5782-333X NR 54 TC 441 Z9 472 U1 2 U2 10 PU MASSACHUSETTS MEDICAL SOC PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD APR 1 PY 1999 VL 340 IS 13 BP 977 EP 987 PG 11 WC Medicine, General & Internal SC General & Internal Medicine GA 182GA UT WOS:000079489800001 ER PT J AU Rabkin, CS Shepherd, FA Wade, JA AF Rabkin, CS Shepherd, FA Wade, JA TI Human herpesvirus 8 and renal transplantation SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter ID KAPOSIS-SARCOMA C1 NCI, Bethesda, MD 20892 USA. Toronto Gen Hosp, Toronto, ON M5G 2C4, Canada. RP Rabkin, CS (reprint author), NCI, Bethesda, MD 20892 USA. NR 4 TC 10 Z9 11 U1 0 U2 0 PU MASSACHUSETTS MEDICAL SOC PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD APR 1 PY 1999 VL 340 IS 13 BP 1045 EP 1046 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 182GA UT WOS:000079489800021 PM 10189288 ER PT J AU Wu, CC Gansow, OA Brechbiel, MW AF Wu, CC Gansow, OA Brechbiel, MW TI Evaluation of methods for large scale preparation of antibody ligand conjugates SO NUCLEAR MEDICINE AND BIOLOGY LA English DT Article ID BIFUNCTIONAL CHELATING AGENT; MONOCLONAL-ANTIBODIES; PROTEIN CONJUGATION; RADIOIMMUNOTHERAPY; THERAPY AB A rapid, single vessel method for preparation of clinical grade monoclonal antibody-chelating agent conjugates has been evaluated. By use sf diafiltration methodology, currently employed dialysis step(s) that are normally used for the production of immunoconjugates may be eliminated. This technique has the advantage of eliminating the use of large amounts of buffer and the several days of time associated with purification of the product conjugate from unreacted ligand by dialysis, reduced risk of metal, pyrogen, and bacterial contamination, all coupled with the ability to produce multi-hundred milligram amounts of product suitable for vialing under GMP conditions for clinical applications. Evaluation of the product by this method indicates a superior quality of purity as compared with immunoconjugates prepared by the established methods. C1 NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, Bethesda, MD 20892 USA. RP Brechbiel, MW (reprint author), NCI, Radioimmune & Inorgan Chem Sect, Radiat Oncol Branch, Bethesda, MD 20892 USA. EM martinwb@box-m.nih.gov NR 17 TC 6 Z9 7 U1 1 U2 4 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0969-8051 J9 NUCL MED BIOL JI Nucl. Med. Biol. PD APR PY 1999 VL 26 IS 3 BP 339 EP 342 DI 10.1016/S0969-8051(98)00112-7 PG 4 WC Radiology, Nuclear Medicine & Medical Imaging SC Radiology, Nuclear Medicine & Medical Imaging GA 182UF UT WOS:000079516000012 PM 10363806 ER PT J AU Aravind, L Koonin, EV AF Aravind, L Koonin, EV TI DNA polymerase beta-like nucleotidyltransferase superfamily: identification of three new families, classification and evolutionary history SO NUCLEIC ACIDS RESEARCH LA English DT Article ID SACCHAROMYCES-CEREVISIAE; GLUTAMINE-SYNTHETASE; SEQUENCE DATABASES; ADENYLATE-CYCLASE; PROTEIN SEQUENCES; CRYSTAL-STRUCTURE; CATALYTIC DOMAIN; TOPOISOMERASE-I; ENZYME; POLYADENYLATION AB A detailed analysis of the pol beta superfamily of nucleotidyltransferases was performed using computer methods for iterative database search, multiple alignment, motif analysis and structural modeling. Three previously uncharacterized families of predicted nucleotidyltransferases are described. One of these new families includes small proteins found in all archaea and some bacteria that appear to consist of the minimal nucleotidyltransferase domain and may resemble the ancestral state of this superfamily. Another new family that is specifically related to eukaryotic polyA polymerases is typified by yeast Trf4p and Trf5p proteins that are involved in chromatin remodeling. The TRF family is represented by multiple members in all eukaryotes and may be involved in yet unknown nucleotide polymerization reactions required for maintenance of chromatin structure, Another new family of bacterial and archaeal nucleotidyltransferases is predicted to function in signal transduction since, in addition to the nucleotidyltransferase domain, these proteins contain ligand-binding domains. It is further shown that the catalytic domain of gamma proteobacterial adenylyl cyclases is homologous to the pol beta superfamily nucleotidyltransferases which emphasizes the general trend for the origin of signal-transducing enzymes from those involved in replication, repair and RNA processing. Classification of the pol beta superfamily into distinct families and examination of their phyletic distribution suggests that the evolution of this type of nucleotidyltransferases may have included bursts of rapid divergence linked to the emergence of new functions as well as a number of horizontal gene transfer events. C1 Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. RP Aravind, L (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. EM aravind@ncbi.nlm.nih.gov NR 57 TC 224 Z9 231 U1 0 U2 4 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR 1 PY 1999 VL 27 IS 7 BP 1609 EP 1618 DI 10.1093/nar/27.7.1609 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 182NG UT WOS:000079504600006 PM 10075991 ER PT J AU Compton, ST Henning, KA Chen, M Mansoura, MK Ashlock, MA AF Compton, ST Henning, KA Chen, M Mansoura, MK Ashlock, MA TI An improved method for routine preparation of intact artificial chromosome DNA (340-1000 kb) for transfection into human cells SO NUCLEIC ACIDS RESEARCH LA English DT Article ID TRANSGENIC MICE; MAMMALIAN-CELLS; MICROINJECTION; GENE; CONSTRUCTION; EXPRESSION; GENERATION; FUSION AB The transfer of high molecular weight (HMW) DNA into mammalian cells is an important strategy for assessing human gene expression and chromosome structure and function. However, using current methods, it is difficult to dependably prepare intact HMW DNA because of the susceptibility of the DNA to degradation and physical shearing. Here we describe a strategy whereby intact artificial chromosome DNA (as large as 1 Mb) can be routinely prepared from yeast. Strict adherence to this protocol has resulted in: (i) >90% of liquid DNA preparations containing largely intact DNA; (ii) transfection efficiencies for the development of stable human clonal cell lines ranging from 5 x 10(-7) to 8.8 x 10(-5); and (iii) the presence of markers from both YAC arms in 30-42% of the human fibrosarcoma cell HT1080 clones and 100% of the CF lung epithelial cell lines IB3-1 and CFT1 clones, suggesting that the HMW DNA is potentially intact in a substantial proportion of clones. Using this protocol for DNA preparation, successful transfection of functional 1 Mb human artificial chromosome DNA into human cells has also been achieved. This methodology should prove useful to those interested in using HMW human DNA for gene expression and functional analysis or for linear artificial chromosome construction, since integrity is absolutely critical for the success of these studies. C1 NIH, Natl Ctr Human Genome Res, Genet & Mol Biol Branch, Vector Dev Sect, Bethesda, MD 20892 USA. RP Ashlock, MA (reprint author), NIH, Natl Ctr Human Genome Res, Genet & Mol Biol Branch, Vector Dev Sect, Bldg 49,Room 3A20,49 Convent Dr,MSC 4442, Bethesda, MD 20892 USA. EM melis@nhgri.nih.gov NR 25 TC 8 Z9 8 U1 0 U2 2 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR 1 PY 1999 VL 27 IS 7 BP 1762 EP 1765 DI 10.1093/nar/27.7.1762 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 182NG UT WOS:000079504600024 PM 10076009 ER PT J AU Yang, HK Kang, SH Kim, YS Won, K Bang, YJ Kim, SJ AF Yang, HK Kang, SH Kim, YS Won, K Bang, YJ Kim, SJ TI Truncation of the TGF-beta type II receptor gene results in insensitivity to TGF-beta in human gastric cancer cells SO ONCOGENE LA English DT Article DE transforming growth factor-beta; cancer; mutation; carcinogenesis; receptor ID MICROSATELLITE INSTABILITY; COLORECTAL CANCERS; GROWTH-INHIBITION; MUTATIONS; COLON; EXPRESSION; KINASE AB The transforming growth factor-beta (TGF-beta receptor system has been implicated in the development of resistance to the growth-inhibitory effects of TGF-beta. It has been reported that resistance to TGF-beta correlates with inactivation of the TGF-beta type II receptor (RII). In the present report, we examine the genetic changes in the TGF-beta RII gene of human gastric cancer cell lines, SNU-5 and SNU-668, which we had previously reported to express truncated TGF-beta RII transcripts. By independent PCR and Southern hybridization analysis of genomic DNA, we found that the genomic sequence of TGF-beta RII is truncated after exon 2 in SNU-5 and after exon 3 in SNU-668. This was confirmed by sequencing the TGF-beta RII cDNA cloned from a SNU-5 cDNA library. Predicted TGF-beta RII protein of SNU-5 cells based on sequencing data contains only a part of extracellular domain of TGF-beta RII. We demonstrate that cotransfection of 3TP-Lux and wild type TGF-beta RII restores the TGF-beta responsiveness in SNU-5 cells, suggesting that genetic changes in the TGF-beta RII gene of SNU-5 cells are responsible for the loss of sensitivity to TGF-beta. This is the first report demonstrating that truncation of the TGF-beta RII gene is an alternative mechanism to inactivate the TGF-beta signal transduction pathways. C1 NCI, Lab Cell Regulat & Carcinogenesis, Bethesda, MD 20892 USA. Seoul Natl Univ, Coll Med, Canc Res Ctr, Seoul, South Korea. RP Kim, SJ (reprint author), NCI, Lab Cell Regulat & Carcinogenesis, Bethesda, MD 20892 USA. RI Yang, Han-Kwang/J-2767-2012; Bang, Yung Jue/J-2759-2012 NR 28 TC 30 Z9 32 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0950-9232 J9 ONCOGENE JI Oncogene PD APR 1 PY 1999 VL 18 IS 13 BP 2213 EP 2219 DI 10.1038/sj.onc.1202535 PG 7 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA 182YE UT WOS:000079525100005 PM 10327067 ER PT J AU Kaplan, RS Smiley, JK Cheson, BD AF Kaplan, RS Smiley, JK Cheson, BD TI Clinical trials referral resource SO ONCOLOGY-NEW YORK LA English DT Editorial Material C1 NCI, Bethesda, MD 20892 USA. RP Kaplan, RS (reprint author), NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU P R R INC PI MELVILLE PA 48 SOUTH SERVICE RD, MELVILLE, NY 11747 USA SN 0890-9091 J9 ONCOLOGY-NY JI Oncology-NY PD APR PY 1999 VL 13 IS 4 BP 532 EP 532 PG 1 WC Oncology SC Oncology GA 368QB UT WOS:000090128000014 PM 10234703 ER PT J AU Seybold, N AF Seybold, N TI At the crossroads: The intersection of the Internet and clinical oncology SO ONCOLOGY-NEW YORK LA English DT Editorial Material C1 NCI, Off Clin Res Promot, Bethesda, MD 20892 USA. RP Seybold, N (reprint author), NCI, Off Clin Res Promot, Bethesda, MD 20892 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU P R R INC PI MELVILLE PA 48 SOUTH SERVICE RD, MELVILLE, NY 11747 USA SN 0890-9091 J9 ONCOLOGY-NY JI Oncology-NY PD APR PY 1999 VL 13 IS 4 BP 585 EP 586 PG 2 WC Oncology SC Oncology GA 368QB UT WOS:000090128000021 ER PT J AU Zierhut, M Wild, U Roser, R Wiggert, B Thiel, HJ Stiemer, R AF Zierhut, M Wild, U Roser, R Wiggert, B Thiel, HJ Stiemer, R TI Experimental autoimmune uveoretinitis: characterization of retina infiltrating cells SO OPHTHALMOLOGE LA German DT Article DE experimental autoimmune uveitis (EAU); interphotoreceptor retinoid binding protein (IRBP); T-cells; macrophages; intercellular adhesion molecule (ICAM) ID ADHESION MOLECULES; T-CELLS; MECHANISMS; DISEASE; UVEITIS; MICE AB The chronic model of murine EAU induced by interphotoreceptor retinoid binding protein represents a disease similar to clinical chorioretinitis. In this study we characterized the kinetics of retina infiltrating T-cells, macrophages and expression of the adhesion molecules ICAM-1 and ICAM-2. Methods: B10.A mice were immunized subcutaneously with IRBP, and the eyes were analyzed on days 10, 18, 24 and 28. The infiltrating cells were characterized by mAbs recognizing T-cell receptors (TCR) V beta 6 acid V beta 8, T-cell markers, macrophages and ICAM-1 and ICAM-2. Results: While CD8(+) T-cells and ICAM-2 were detectable from day 10 (retina is intact) until day 28, CD4(+) T-cells, macrophages and ICAM-1 appear with the onset of retinal destruction. Starting at day 10 the dominating TCR was V beta 6; V beta 8 was noticed from day 18 on. Conclusion: CD8(+) T-cells infiltrating the intact retina and stimulating the expression of high endothelial venules (HEVs) could be responsiable for the onset of uveitis. C1 Univ Tubingen, Abt 1, Augenklin, D-72076 Tubingen, Germany. NEI, NIH, Bethesda, MD 20892 USA. RP Zierhut, M (reprint author), Univ Tubingen, Abt 1, Augenklin, Schleichstr 12-16, D-72076 Tubingen, Germany. NR 20 TC 1 Z9 1 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0941-293X J9 OPHTHALMOLOGE JI Ophthalmologe PD APR PY 1999 VL 96 IS 4 BP 252 EP 256 DI 10.1007/s003470050401 PG 5 WC Ophthalmology SC Ophthalmology GA 192TL UT WOS:000080095300005 PM 10409853 ER PT J AU Barmes, DE AF Barmes, DE TI Symposium on noma: Foreword SO ORAL DISEASES LA English DT Editorial Material C1 Natl Inst Dent & Craniofacial Res, Off Int Hlth, NIH, Bethesda, MD 20892 USA. RP Barmes, DE (reprint author), Natl Inst Dent & Craniofacial Res, Off Int Hlth, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 1354-523X J9 ORAL DIS JI Oral Dis. PD APR PY 1999 VL 5 IS 2 BP 143 EP 143 PG 1 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA 256WL UT WOS:000083749400010 ER PT J AU Brennan, MT Patronas, NJ Brahim, JS AF Brennan, MT Patronas, NJ Brahim, JS TI Bilateral condylar resorption in dermatomyositis - A case report SO ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS LA English DT Article ID TEMPOROMANDIBULAR-JOINT; NECK MANIFESTATIONS; MANDIBULAR CONDYLES; SYNOVIAL-FLUID; POLYMYOSITIS; CARTILAGE; DISEASE; GLUCOCORTICOIDS; INVOLVEMENT; HEAD AB Polymyositis is an inflammatory disease commonly affecting the striated muscle. When it is accompanied by characteristic skin lesions, the condition is called dermatomyositis, Bilateral condylar resorption has been reported with autoimmune conditions and chronic systemic steroids. We report the first documented case of bilateral condylar resorption in a patient with dermatomyositis. Possible etiologic factors and treatment outcomes are discussed. C1 NIDR, NIH, Bethesda, MD 20892 USA. NIH, Ctr Clin, Neuroradiol Sect, Bethesda, MD 20892 USA. NIH, Dept Diagnost Radiol, Bethesda, MD 20892 USA. RP Brahim, JS (reprint author), NIDR, NIH, Bldg 10 Room 1N-113,10 Ctr Dr MSC 1190, Bethesda, MD 20892 USA. NR 25 TC 7 Z9 7 U1 0 U2 1 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 USA SN 1079-2104 J9 ORAL SURG ORAL MED O JI Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. PD APR PY 1999 VL 87 IS 4 BP 446 EP 451 DI 10.1016/S1079-2104(99)70244-1 PG 6 WC Dentistry, Oral Surgery & Medicine SC Dentistry, Oral Surgery & Medicine GA 185UB UT WOS:000079687900011 PM 10225627 ER PT J AU Hrabie, JA Saavedra, JE Davies, KM Keefer, LK AF Hrabie, JA Saavedra, JE Davies, KM Keefer, LK TI Adducts of piperazine with nitric oxide SO ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL LA English DT Article C1 NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Chem Synth & Anal Lab, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Intramural Res Support Program, Frederick, MD 21702 USA. George Mason Univ, Dept Chem, Fairfax, VA 22030 USA. NCI, Frederick Canc Res & Dev Ctr, Chem Sect, Comparat Carcinogenesis Lab, Frederick, MD 21702 USA. RP Hrabie, JA (reprint author), NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Chem Synth & Anal Lab, Frederick, MD 21702 USA. RI Keefer, Larry/N-3247-2014 OI Keefer, Larry/0000-0001-7489-9555 NR 7 TC 10 Z9 10 U1 0 U2 0 PU ORGANIC PREP PROCEDURES INC PI NEWTON HIGHLANDS PA PO BOX 9, NEWTON HIGHLANDS, MA 02161 USA SN 0030-4948 J9 ORG PREP PROCED INT JI Org. Prep. Proced. Int. PD APR PY 1999 VL 31 IS 2 BP 189 EP 192 PG 4 WC Chemistry, Organic SC Chemistry GA 188PE UT WOS:000079856400006 ER PT J AU Ramesha, AR Bhat, S Prabhu, KR AF Ramesha, AR Bhat, S Prabhu, KR TI Isomerization of longifolene to isolongifolene catalyzed by montmorillonite clay SO ORGANIC PREPARATIONS AND PROCEDURES INTERNATIONAL LA English DT Article C1 Ray Chem Private Ltd, R&D Ctr, Bangalore 560064, Karnataka, India. Indian Inst Sci, Dept Organ Chem, Bangalore 560012, Karnataka, India. RP Ramesha, AR (reprint author), NIDDK, NIH, Bldg 8,Room 1A05, Bethesda, MD 20892 USA. RI Bhat, Shridhar/C-5412-2012 NR 12 TC 0 Z9 0 U1 1 U2 3 PU ORGANIC PREP PROCEDURES INC PI NEWTON HIGHLANDS PA PO BOX 9, NEWTON HIGHLANDS, MA 02161 USA SN 0030-4948 J9 ORG PREP PROCED INT JI Org. Prep. Proced. Int. PD APR PY 1999 VL 31 IS 2 BP 227 EP 230 PG 4 WC Chemistry, Organic SC Chemistry GA 188PE UT WOS:000079856400017 ER PT J AU Broniatowski, M Sonies, BC Rubin, JS Bradshaw, CR Spiegel, JR Bastian, RW Kelly, JH AF Broniatowski, M Sonies, BC Rubin, JS Bradshaw, CR Spiegel, JR Bastian, RW Kelly, JH TI Current evaluation and treatment of patients with swallowing disorders SO OTOLARYNGOLOGY-HEAD AND NECK SURGERY LA English DT Article; Proceedings Paper CT 100th Annual Meeting of the American-Academy-of-Otolaryngology-Head-and-Neck-Surgery CY SEP 29-OCT 02, 1996 CL WASHINGTON, D.C. SP Amer Acad Otolaryngol Head & Neck Surg ID ASPIRATION; DYSPHAGIA; SURGERY AB To determine the varied causes of oropharyngeal dysphagia and their respective pathophysiology, a working understanding of the normal anatomy and function of the highly integrated mechanism of swallowing is outlined. This information is presented as the basis for a reasoned and detailed approach to the history, physical examination, and endoscopic evaluation of normal and altered oropharyngeal swallowing. The management of swallowing disorders depends on the nature and magnitude of the responsible clinical condition. Conservative and surgical approaches are discussed. These modalities and their indications are described in detail. C1 Cleveland Clin, Hlth Sci Ctr, Cleveland, OH 44106 USA. Caritas St Vincent Charity Hosp, Bethesda, MD USA. NIH, Warren G Magnuson Clin Ctr, Bethesda, MD 20892 USA. NIH, Dept Rehabil Med, Bethesda, MD 20892 USA. Royal Natl Throat Nose & Ear Hosp, London WC1X 8DA, England. Lewisham Univ Hosp, Lewisham NHS Trust, Philadelphia, PA USA. Thomas Jefferson Univ, Philadelphia, PA 19107 USA. Grad Hosp, Dept Otolaryngol Head & Neck Surg, Philadelphia, PA 19107 USA. Loyola Univ, Dept Otolaryngol Head & Neck Surg, Chicago, IL 60611 USA. Johns Hopkins Med Inst, Baltimore, MD 21205 USA. Greater Baltimore Med Ctr, Dept Otolaryngol Head & Neck Surg, Baltimore, MD USA. RP Broniatowski, M (reprint author), 2351 E 22nd St, Cleveland, OH 44115 USA. NR 35 TC 23 Z9 27 U1 0 U2 3 PU MOSBY-YEAR BOOK INC PI ST LOUIS PA 11830 WESTLINE INDUSTRIAL DR, ST LOUIS, MO 63146-3318 USA SN 0194-5998 J9 OTOLARYNG HEAD NECK JI Otolaryngol. Head Neck Surg. PD APR PY 1999 VL 120 IS 4 BP 464 EP 473 DI 10.1053/hn.1999.v120.a93228 PG 10 WC Otorhinolaryngology; Surgery SC Otorhinolaryngology; Surgery GA 184DF UT WOS:000079594100005 PM 10187935 ER PT J AU Cheng, TL Lomax, T Fields, CB Wright, JL Brenner, RA Scheidt, PC AF Cheng, TL Lomax, T Fields, CB Wright, JL Brenner, RA Scheidt, PC TI Sports injuries: A major cause of morbidity in urban youth SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 George Washington Univ, Childrens Natl Med Ctr, Childrens Res Inst, Dept Gen Pediat & Adolescent Med, Washington, DC 20052 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 5 BP 2A EP 2A DI 10.1203/00006450-199904020-00022 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700006 ER PT J AU Scheldt, PC Overpeck, M Ross, J Harel, Y Marshall, L Rouse, B AF Scheldt, PC Overpeck, M Ross, J Harel, Y Marshall, L Rouse, B TI Prevalence and correlations of fighting behavior in a nationally representative sample of young adolescents SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Natl Med Ctr, Washington, DC 20010 USA. NICHHD, Bethesda, MD 20892 USA. Macro Int, Calverton, MD USA. Bar Ilan Univ, Ramat Gan, Israel. ISR, Substance Abuse & Mental Hlth Serv Adm, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 26 BP 6A EP 6A DI 10.1203/00006450-199904020-00043 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700027 ER PT J AU Sovik, KM Nguyen, TT Keil, M Pathomvanich, A Field, A Yanovski, JA AF Sovik, KM Nguyen, TT Keil, M Pathomvanich, A Field, A Yanovski, JA TI Self-assessment of pubertal maturation in overweight African American and Caucasian children SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, NIH, DEB, UGO, Bethesda, MD USA. NIH, DDDN, Bethesda, MD 20892 USA. Harvard Univ, Brigham & Womens Hosp, Sch Med, Channing Lab, Boston, MA 02115 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 30 BP 7A EP 7A DI 10.1203/00006450-199904020-00047 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700031 ER PT J AU Shankaran, S Lester, BM Langer, JC Bauer, CR Bada, HS Tronic, EJ Seifer, R Wright, LL Smeriglio, V AF Shankaran, S Lester, BM Langer, JC Bauer, CR Bada, HS Tronic, EJ Seifer, R Wright, LL Smeriglio, V TI Maternal lifestyle study (MLS): Relationship of neurologic exam to neurobehavior following cocaine-opiate exposure SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Wayne State Univ, Detroit, MI 48202 USA. Brown Univ, Providence, RI 02912 USA. Univ Miami, Coral Gables, FL 33124 USA. Univ Tennessee, Knoxville, TN 37996 USA. NICHD, Bethesda, MD USA. NIDA, Lexington, KY 40853 USA. RTI, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 88 BP 17A EP 17A DI 10.1203/00006450-199904020-00105 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700089 ER PT J AU Khan, S Huber, R Schlessinger, D Fant, M AF Khan, S Huber, R Schlessinger, D Fant, M TI Glypican-3 (GPC3), a heparan sulfate proteoglycan associated with the Simpson-Golabi-Behmel fetal overgrowth syndrome, is expressed by the syncytiotrophoblast in the human placenta SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NIA, Genet Lab, NIH, Baltimore, MD 21224 USA. Univ Texas, Sch Med, Dept Pediat, Houston, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 310 BP 54A EP 54A DI 10.1203/00006450-199904020-00327 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700311 ER PT J AU McCune, SK Spong, CY Brenneman, DE Hill, JM AF McCune, SK Spong, CY Brenneman, DE Hill, JM TI Expression of PACAP receptor splice variant mRNAs in the developing mouse CNS SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Natl Med Ctr, Dept Neonatol, Washington, DC 20010 USA. NICHD, Sect Dev & Mol Pharmacol, LDN, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 324 BP 57A EP 57A DI 10.1203/00006450-199904020-00341 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700325 ER PT J AU Abad, V De Luca, F Uyeda, JA Bacher, JD Baron, J AF Abad, V De Luca, F Uyeda, JA Bacher, JD Baron, J TI Spatial orientation of growth plate chondrocytes SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NIH, Vet Resources Program, Dev Endocrinol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 480 BP 83A EP 83A DI 10.1203/00006450-199904020-00497 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700481 ER PT J AU Lafferty, AR Torpy, DJ Huggard, P Gordon, RD Stratakis, CA AF Lafferty, AR Torpy, DJ Huggard, P Gordon, RD Stratakis, CA TI Familial hyperaldosteronism type II: Identification of potential disease loci using linkage analysis SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, DEB, Sect Pediat Endocrinol, NIH, Bethesda, MD USA. Queen Elizabeth Hosp, Dept Endocrinol & Diabet, Adelaide, SA, Australia. Greenslopes Hosp, Dept Endocrinol, Brisbane, Qld, Australia. RI Gordon, Richard/K-2555-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 534 BP 92A EP 92A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700535 ER PT J AU Lafferty, AR Patronas, NJ Goldberg, P Oldfield, EH Stratakis, CA AF Lafferty, AR Patronas, NJ Goldberg, P Oldfield, EH Stratakis, CA TI The diagnostic accuracy of pituitary magnetic resonance imaging is enhanced by the use of fast spoiled GRASS SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Natl Inst Hlth, Bethesda, MD USA. RI Gordon, Richard/K-2555-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 533 BP 92A EP 92A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700534 ER PT J AU Yanovski, JA Diament, AL Sovik, KN Nguyen, TT Yanovski, SZ Li, H Sebring, N Warden, CH AF Yanovski, JA Diament, AL Sovik, KN Nguyen, TT Yanovski, SZ Li, H Sebring, N Warden, CH TI Association between uncoupling protein 2, body composition, and resting energy expenditure in lean and obese African American, Asian, and Caucasian children SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Univ Calif Davis, Davis, CA 95616 USA. NIH, Bethesda, MD 20892 USA. OI Yanovski, Jack/0000-0001-8542-1637 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 578 BP 100A EP 100A DI 10.1203/00006450-199904020-00595 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700579 ER PT J AU Brenner, R Trumble, AC Overpeck, MD Smith, GS Kessler, E AF Brenner, R Trumble, AC Overpeck, MD Smith, GS Kessler, E TI Childhood drownings - What else do we know? SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHHD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 584 BP 101A EP 101A DI 10.1203/00006450-199904020-00601 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700585 ER PT J AU Corwin, MJ Bak, S Willinger, M Hoffman, HJ Kessler, RC AF Corwin, MJ Bak, S Willinger, M Hoffman, HJ Kessler, RC TI Use of soft bedding among infants in the US SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Boston Univ, Med Ctr, Sch Med, Boston, MA 02118 USA. Boston Univ, Sch Publ Hlth, Med Ctr, Boston, MA USA. NICHD, Pregnancy & Perinatol Branch, NIH, Bethesda, MD USA. NICHD, Stat & Data Syst Branch, NIH, Bethesda, MD USA. Harvard Univ, Sch Med, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 588 BP 102A EP 102A DI 10.1203/00006450-199904020-00605 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700589 ER PT J AU Rogan, WJ AF Rogan, WJ CA TLC Investigators TI Treatment of lead-exposed children (TLC) trial: Effect of succimer in toddlers with blood lends of 20-44 mu g/dl SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NIEHS, Res Triangle Pk, NC 27709 USA. RI Rogan, Walter/I-6034-2012 OI Rogan, Walter/0000-0002-9302-0160 NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 614 BP 106A EP 106A DI 10.1203/00006450-199904020-00631 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700615 ER PT J AU Allikmets, R Raskind, WH Hutchinson, A Schueck, ND Dean, M Koeller, DM AF Allikmets, R Raskind, WH Hutchinson, A Schueck, ND Dean, M Koeller, DM TI Mutation of a putative mitochondrial iron transporter gene (ABC7) in X-linked sideroblastic anemia and ataxia (XLSA/A) SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Natl Canc Inst, Lab Genom Divers, Frederick, MD USA. Univ Washington, Dept Med, Seattle, WA USA. Univ Colorado, Hlth Sci Ctr, Dept Pediat, Denver, CO 80262 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 788 BP 135A EP 135A DI 10.1203/00006450-199904020-00805 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700789 ER PT J AU Anikster, Y Lucero, C Touchman, JW Huizing, M McDowel, G Shotelersuk, V Green, ED Gahl, WA AF Anikster, Y Lucero, C Touchman, JW Huizing, M McDowel, G Shotelersuk, V Green, ED Gahl, WA TI Breakpoint identification, detection and frequency of the 65-kb deletion in the cystinosis gene, CTNS SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Sect Human Biochem Genet, HDB, NIH, Bethesda, MD USA. NHGRI, Med Genet Branch, NIH, Bethesda, MD USA. NHGRI, Genome Technol Branch, NIH, Bethesda, MD USA. NIH, NIH Intramural Sequencing Ctr, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 790 BP 136A EP 136A DI 10.1203/00006450-199904020-00807 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700791 ER PT J AU Bouma, P Cole, WG Sidbury, JB Marini, JC AF Bouma, P Cole, WG Sidbury, JB Marini, JC TI A null alpha 1(V) collagen allele is caused by an intronic insertion in a family with Ehlers-Danlos Syndrome II SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Hosp Sick Children, Div Orthopaed, Toronto, ON M5G 1X8, Canada. NICHD, Heritable Disorders Branch, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 792 BP 136A EP 136A DI 10.1203/00006450-199904020-00809 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700793 ER PT J AU Enns, GM Seppala, R Musci, TJ Weisiger, K Ferrell, LD Wenger, DA Gahl, WA Packman, S AF Enns, GM Seppala, R Musci, TJ Weisiger, K Ferrell, LD Wenger, DA Gahl, WA Packman, S TI Clinical and molecular studies in sialuria SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Univ Calif San Francisco, Dept Peds Path, San Francisco, CA 94143 USA. NICHD, Herit Disord Br, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 800 BP 137A EP 137A DI 10.1203/00006450-199904020-00817 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700801 ER PT J AU Gahl, WA Shotelersuk, V Brantly, M Huizing, M Dell'Angelica, EC Bonifacino, JS AF Gahl, WA Shotelersuk, V Brantly, M Huizing, M Dell'Angelica, EC Bonifacino, JS TI Hermansky-Pudlak syndrome (HPS) as a genetically heterogeneous disease: Clinical, molecular, and cell biological aspects SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Heritable Disorders Branch, NIH, Bethesda, MD USA. NHLBI, Pulm Crit Care Med Branch, NIH, Bethesda, MD 20892 USA. NICHD, Cell Biol & Metab Branch, NIH, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 804 BP 138A EP 138A DI 10.1203/00006450-199904020-00821 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700805 ER PT J AU Marini, JC Forlino, A Dawson, PA AF Marini, JC Forlino, A Dawson, PA TI Development of gene therapy for osteogenesis imperfecta using hammerhead ribozymes and a knock-in mouse model of OI SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Heritable Disorders Branch, NIH, Bethesda, MD USA. RI Dawson, Paul/B-1268-2012; Forlino, Antonella/H-5385-2015 OI Forlino, Antonella/0000-0002-6385-1182 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 811 BP 139A EP 139A DI 10.1203/00006450-199904020-00828 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700812 ER PT J AU Orvisky, E Martin, BM Tayebi, N Karson, E Krasnewich, D Ginns, EI Sidransky, E AF Orvisky, E Martin, BM Tayebi, N Karson, E Krasnewich, D Ginns, EI Sidransky, E TI Glucosylsphingosine accumulation in type 2 Gaucher disease begins early in gestation SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Columbia Hosp Women, Washington, DC USA. NIMH, IRP, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 815 BP 140A EP 140A DI 10.1203/00006450-199904020-00832 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700816 ER PT J AU Porter, FD Steiner, RD Nwokoro, NA Tsokos, M Maslen, C Wassif, CA AF Porter, FD Steiner, RD Nwokoro, NA Tsokos, M Maslen, C Wassif, CA TI Cellular and molecular studies of Smith-Lemli-Opitz Syndrome SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, HDB, NIH, Bethesda, MD USA. NCI, NIH, Bethesda, MD 20892 USA. Oregon Hlth Sci Univ, Portland, OR 97201 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 818 BP 140A EP 140A DI 10.1203/00006450-199904020-00835 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700819 ER PT J AU Stone, D Coble, C Tayebi, N Sidransky, E AF Stone, D Coble, C Tayebi, N Sidransky, E TI Cardiovascular fibrosis, hydrocephalus, oculomotor abnormalities and visceral involvement associated with mutation D409H in an American child with Gaucher disease SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NIMH, DIRP, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 823 BP 141A EP 141A DI 10.1203/00006450-199904020-00840 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700824 ER PT J AU Tayebi, N Stubblefield, BK Koprivica, V Callahan, MB Stone, D Sidransky, E AF Tayebi, N Stubblefield, BK Koprivica, V Callahan, MB Stone, D Sidransky, E TI Different crossover and gene conversion events within the glucocerebrosidase region contribute to the heterogeneity encountered in Gaucher disease SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NIMH, DIRP, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 824 BP 141A EP 141A DI 10.1203/00006450-199904020-00841 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700825 ER PT J AU Jubran, RF Dickstein, B Buu, M Binh, TV Be, TV Young, NS Luban, NLC Brown, KE AF Jubran, RF Dickstein, B Buu, M Binh, TV Be, TV Young, NS Luban, NLC Brown, KE TI TTV viremia is common in healthy American and Vietnamese children SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Natl Med Ctr, Washington, DC 20010 USA. NHLBI, Hematol Branch, Bethesda, MD 20892 USA. Blood Transfus & Hematol Ctr, Ho Chi Minh City, Vietnam. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 861 BP 148A EP 148A DI 10.1203/00006450-199904020-00878 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700862 ER PT J AU Black, J Xiang, C Chen, Y Gillim, L Gooden, G Leighton, S Meltzer, P Newcomb, F Pohida, T Smith, P Trent, J Yarchoan, R Zeichner, S AF Black, J Xiang, C Chen, Y Gillim, L Gooden, G Leighton, S Meltzer, P Newcomb, F Pohida, T Smith, P Trent, J Yarchoan, R Zeichner, S TI Expression analysis of herpesvirus transcription programs using cDNA microarrays SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NCI, HAMB, NIH, Bethesda, MD 20892 USA. NHGRI, LCG, NIH, Bethesda, MD 20892 USA. NIH, BEIP, Bethesda, MD 20892 USA. NIH, DCRT, Bethesda, MD 20892 USA. NIH, NCRR, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 914 BP 157A EP 157A DI 10.1203/00006450-199904020-00931 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700915 ER PT J AU Gruber, WC Belshe, RB Treanor, J Mendelman, PM Wolff, M AF Gruber, WC Belshe, RB Treanor, J Mendelman, PM Wolff, M TI Efficacy of trivalent live attenuated intranasal influenza vaccine against monovalent influenza H1N1 challenge in children SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Vanderbilt Univ, NIAID, Vaccine Unit, Nashville, TN USA. Vanderbilt Univ, NIAID, Treatment Evaluat Unit, Nashville, TN USA. St Louis Univ, St Louis, MO 63103 USA. Univ Rochester, Rochester, NY 14627 USA. Aviron, Mt View, CA USA. Emmes Corp, Potomac, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 944 BP 162A EP 162A DI 10.1203/00006450-199904020-00961 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700945 ER PT J AU Lin, FY Weisman, LE Azimi, PH Regan, JA Philips, JB Clark, P Rhoads, GG Kong, FH Brenner, R Clemens, J AF Lin, FY Weisman, LE Azimi, PH Regan, JA Philips, JB Clark, P Rhoads, GG Kong, FH Brenner, R Clemens, J TI Determinants of early-onset Group B streptococcal disease - A multi center case-control study SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Hosp, Baylor C Med, NICHD, NIH, Oakland, CA 94609 USA. Univ Alabama, Birmingham, AL USA. Columbia Univ, New York, NY 10027 USA. Univ Florida, Gainesville, FL 32611 USA. Univ Med & Dent New Jersey, Westat, NJ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 973 BP 167A EP 167A DI 10.1203/00006450-199904020-00990 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700974 ER PT J AU Mirochnick, M Cooper, E Xu, J Lindsey, J Capparelli, E McIntosh, K McNamara, J Mofenson, LM Jacobus, D AF Mirochnick, M Cooper, E Xu, J Lindsey, J Capparelli, E McIntosh, K McNamara, J Mofenson, LM Jacobus, D CA PACTG Protocol 179 Team TI Population pharmacokinetics of dapsone in HIV-infected children SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Boston Univ, Sch Med, Boston, MA 02118 USA. Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. Univ Calif San Diego, San Diego, CA 92103 USA. Harvard Univ, Sch Med, Boston, MA USA. NICHD, NIAID, Jacobus Pharmaceut Co, Princeton, NJ USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 982 BP 168A EP 168A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476700983 ER PT J AU Stiehm, ER Fletcher, C Mofenson, LM AF Stiehm, ER Fletcher, C Mofenson, LM CA PACTG 273 Protocol Grp TI Human hyperimmune HIV intravenous immunoglobulin (HIV-IG) in the treatment of HIV-I infection in children (PACTG-273) SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Univ Calif Los Angeles, Los Angeles, CA USA. Univ Minnesota, Minneapolis, MN USA. NIAID, Washington, DC USA. NICHD, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1024 BP 175A EP 175A DI 10.1203/00006450-199904020-01041 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701025 ER PT J AU Viraraghavan, R Pikis, A Rakusan, TA Patel, KM Rodriguez, WJ AF Viraraghavan, R Pikis, A Rakusan, TA Patel, KM Rodriguez, WJ TI Nasopharyngeal carriage of Streptococcus pneumoniae: Is there a difference between HIV-infected patients and non-HIV well children? A 3 year outpatient experience SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Natl Med Ctr, Washington, DC 20010 USA. US FDA, CDER, Div Special Pathogens & Immunol Drug Prod, Rockville, MD 20857 USA. NIDCR, Oral Infect & Immun Branch, NIH, Bethesda, MD USA. Childrens Natl Med Ctr, Childrens Res Inst, Washington, DC 20010 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1035 BP 177A EP 177A DI 10.1203/00006450-199904020-01052 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701036 ER PT J AU Wierzba, TF Hall, E Abu-Elvazeed, RR Savarino, SJ Frenck, RW Rao, MR Naticy, AB Morsy, BZ Clemens, JD AF Wierzba, TF Hall, E Abu-Elvazeed, RR Savarino, SJ Frenck, RW Rao, MR Naticy, AB Morsy, BZ Clemens, JD TI Human milk antibody titers to enterotoxigenic Escherichia coli (ETEC) antigens among 118 lactating Egyptian women SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 USN, Med Res Unit 3, Res Sci Dept, Cairo, Egypt. NICHHD, Bethesda, MD 20892 USA. Minist Hlth & Populat, Hlth Serv, Abu Homos, Beheria, Egypt. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1040 BP 178A EP 178A DI 10.1203/00006450-199904020-01057 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701041 ER PT J AU Lemons, JA Stevenson, DK Poole, K Bearden-Branch, A Bauer, CR Papile, LA Korones, SB Stoll, BJ Oh, W Wright, LL AF Lemons, JA Stevenson, DK Poole, K Bearden-Branch, A Bauer, CR Papile, LA Korones, SB Stoll, BJ Oh, W Wright, LL TI In utero magnesium exposure: Effects on extremely low birth weight (ELBW) infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1212 BP 207A EP 207A DI 10.1203/00006450-199904020-01229 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701213 ER PT J AU Lin, FY Weisman, LE Azimi, PH Regan, JA Philips, JB Clark, P Rhoads, G Pratt, E Brenner, R Clemens, J Gill, V AF Lin, FY Weisman, LE Azimi, PH Regan, JA Philips, JB Clark, P Rhoads, G Pratt, E Brenner, R Clemens, J Gill, V TI Antibiotic susceptibility of invasive group B streptococci from neonates with early-onset disease - A multi center study 1995-97 SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Hosp, Oakland, CA 94609 USA. Univ Alabama, Birmingham, AL USA. Univ Florida, Gainesville, FL 32611 USA. Univ Med & Dent New Jersey, Newark, NJ 07103 USA. Westat, Rockville, MD 20850 USA. NIH, Bethesda, MD 20892 USA. Baylor Coll Med, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1220 BP 208A EP 208A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701221 ER PT J AU Oh, W Lemons, JA Stevenson, DK Poole, K Bauer, CR Papile, LA Korones, SB Stoll, BJ Wright, LL AF Oh, W Lemons, JA Stevenson, DK Poole, K Bauer, CR Papile, LA Korones, SB Stoll, BJ Wright, LL TI The effects of low dose indomethacin prophylaxis (LDIP) in extremely low birth weight (ELBW) infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1266 BP 216A EP 216A DI 10.1203/00006450-199904020-01283 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701267 ER PT J AU Ohls, RK Ehrenkranz, RA Lemons, JA Korones, SB Stoll, BJ Stark, AR Wright, LL Shankaran, S Donovan, EF Zimmerman, N AF Ohls, RK Ehrenkranz, RA Lemons, JA Korones, SB Stoll, BJ Stark, AR Wright, LL Shankaran, S Donovan, EF Zimmerman, N TI A multicenter randomized double-masked placebo-controlled trial of early erythropoietin and iron administration to preterm infants SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHHD, Neonatal Res Network, Bethesda, MD 20892 USA. NR 0 TC 10 Z9 10 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1268 BP 216A EP 216A DI 10.1203/00006450-199904020-01285 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701269 ER PT J AU Bada, HS Bauer, CR Shankaran, S Lester, BM Wright, LL Smeriglio, V Poole, K Saha, S Finnegan, L Maza, P AF Bada, HS Bauer, CR Shankaran, S Lester, BM Wright, LL Smeriglio, V Poole, K Saha, S Finnegan, L Maza, P TI Maternal lifestyle study (MLS): Children exposed to cocaine/opiates in utero - Outcome at two years SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Univ Tennessee, Memphis, TN USA. Univ Miami, Coral Gables, FL 33124 USA. Wayne State Univ, Detroit, MI 48202 USA. Brown Univ, Providence, RI 02912 USA. NIDA, Lexington, KY 40583 USA. RTI, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1388 BP 236A EP 236A DI 10.1203/00006450-199904020-01405 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701389 ER PT J AU Bauer, CR Messinger, D Lester, BM Wright, LL Shankaran, S Bada, HS LaGasse, LL Das, A Beeghly, M AF Bauer, CR Messinger, D Lester, BM Wright, LL Shankaran, S Bada, HS LaGasse, LL Das, A Beeghly, M TI Maternal lifestyles study (MLS): Prenatal cocaine/opiate (C/O) exposure is unrelated to changes in Bayley II performance between one and two years. SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Univ Miami, Miami, FL 33152 USA. Brown Univ, Providence, RI 02912 USA. NICHD, Bethesda, MD USA. Wayne State Univ, Detroit, MI USA. Univ Tennessee, Memphis, TN USA. Res Triangle Inst, Res Triangle Pk, NC 27709 USA. Childrens Hosp, Boston, MA 02115 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1393 BP 237A EP 237A DI 10.1203/00006450-199904020-01410 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701394 ER PT J AU LaGasse, LL Seifer, R Wright, LL Lester, BM Tronick, EZ Bauer, CR Shankaran, S Bada, HS Smeriglio, V AF LaGasse, LL Seifer, R Wright, LL Lester, BM Tronick, EZ Bauer, CR Shankaran, S Bada, HS Smeriglio, V TI The maternal lifestyle study (MLS): The caretaking environment of infants exposed to cocaine/opiates SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NIDA, ACYF, CSAT, NICHD Neonatal Res Network, Bethesda, MD USA. NR 0 TC 20 Z9 20 U1 0 U2 3 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1453 BP 247A EP 247A DI 10.1203/00006450-199904020-01470 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701454 ER PT J AU Lester, BM Seifer, R Tronick, EZ LaGasse, LL Bauer, CR Shankaran, S Bada, HS Wright, LL AF Lester, BM Seifer, R Tronick, EZ LaGasse, LL Bauer, CR Shankaran, S Bada, HS Wright, LL TI The maternal lifestyle study (MLS): Patterns of motor development over the first 18 months corrected age in infants exposed to cocaine/opiates SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NIDA, ACYF, CSAT, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1461 BP 248A EP 248A DI 10.1203/00006450-199904020-01478 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701462 ER PT J AU Seifer, R Lester, BM LaGasse, LL Tronick, EZ Bauer, CR Shankaran, S Bada, HS Wright, LL Smeriglio, V AF Seifer, R Lester, BM LaGasse, LL Tronick, EZ Bauer, CR Shankaran, S Bada, HS Wright, LL Smeriglio, V TI The maternal lifestyle Study (MLS): Attachment classification at 18 months corrected age in infants exposed to cocaine/opiates SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 NICHD, Neonatal Res Network, Bethesda, MD USA. NIDA, ACYF, CSAT, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1499 BP 255A EP 255A DI 10.1203/00006450-199904020-01516 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701500 ER PT J AU Vohr, BR Wright, LL Poole, K AF Vohr, BR Wright, LL Poole, K TI Effects of site differences at 12 participating NICHD centers on 18 month outcomes of extremely low birth weight (ELBW) < 100 grams infants - The NICHD neonatal research network follow-up study SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Brown Univ, Sch Med, Providence, RI 02912 USA. Women & Infants Hosp Rhode Isl, Providence, RI 02908 USA. NICHD, Neonatal Res Network Followup Study, Bethesda, MD USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1520 BP 258A EP 258A DI 10.1203/00006450-199904020-01537 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701521 ER PT J AU Sokol, GM Wright, LL Ehrenkranz, RA AF Sokol, GM Wright, LL Ehrenkranz, RA TI Effect of weaning inhaled nitric oxide (INO) on arterial carbon dioxide tension (pCO2) and pH SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Indiana Univ, Sch Med, Indianapolis, IN USA. NICHD, Neonatal Res Network, Bethesda, MD USA. Yale Univ, Sch Med, New Haven, CT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1894 BP 321A EP 321A DI 10.1203/00006450-199904020-01910 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701894 ER PT J AU Torday, JS Londos, C Schultz, CJ Rubin, LP AF Torday, JS Londos, C Schultz, CJ Rubin, LP TI Distension of the developing lung triggers coordinate, growth factor-mediated cell-cell signal transduction SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Harbor UCLA Med Ctr, Torrance, CA 90509 USA. NIDDK, NIH, Bethesda, MD USA. Brown Univ, Providence, RI 02912 USA. OI Torday, John/0000-0001-9071-3052 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1903 BP 322A EP 322A DI 10.1203/00006450-199904020-01919 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701903 ER PT J AU Torday, JS Sunday, ME Londos, C Schultz, CJ Sanchez-Esteban, J Rubin, LP AF Torday, JS Sunday, ME Londos, C Schultz, CJ Sanchez-Esteban, J Rubin, LP TI Mechanomolecular-disregulation of cell-cell signalling pathways in bronchopulmonary dysplasia chronic lung disease SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Harbor UCLA Med Ctr, Torrance, CA 90509 USA. Harvard Univ, Sch Med, Boston, MA USA. NIDDK, NIH, Bethesda, MD USA. Brown Univ, Providence, RI 02912 USA. OI Torday, John/0000-0001-9071-3052 NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1904 BP 323A EP 323A DI 10.1203/00006450-199904020-01920 PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701904 ER PT J AU Izeybigie, EB Gutkind, SJ Ray, PE AF Izeybigie, EB Gutkind, SJ Ray, PE TI Angiotensin II and basic fibroblast growth factor modulate the proliferation of human fetal mesangial cells through different signaling pathways SO PEDIATRIC RESEARCH LA English DT Meeting Abstract C1 Childrens Natl Med Ctr, Childrens Res Inst, Washington, DC 20010 USA. NIDR, OPCB, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 MA 1966 BP 333A EP 333A PN 2 PG 1 WC Pediatrics SC Pediatrics GA 182AQ UT WOS:000079476701966 ER PT J AU Kouraklis, G Triche, TJ Wesley, R Tsokos, M AF Kouraklis, G Triche, TJ Wesley, R Tsokos, M TI Myc oncogene expression and nude mouse tumorigenicity and metastasis formation are higher in alveolar than embryonal rhabdomyosarcoma cell lines SO PEDIATRIC RESEARCH LA English DT Article ID C-MYC; INTERGROUP RHABDOMYOSARCOMA; N-MYC; CHILDHOOD RHABDOMYOSARCOMA; SOLID TUMORS; GENE; DIFFERENTIATION; SARCOMAS; CLASSIFICATION; AMPLIFICATION AB Accumulated clinical evidence suggests that alveolar rhabdomyosarcoma (ARMS) is more aggressive than embryonal rhabdomyosarcoma (ERMS). Here, we study six childhood rhabdomyosarcoma cell lines, three ERMS and three ARMS. We have assayed the ability of the tumor cells to grow in culture and in nude mice as well as their propensity for pulmonary metastasis formation by tail vein injection. We also compared levels of c- and N-myc oncogene expression and DNA copy number. We iind no correlation of histologic tumor type (i.e. ERMS versus ARMS with growth rate in culture, but we do find suggestive correlations of histologic type with tumorigenicity (mean tumor diameter in millimeters at 6 wk: ARMS 30, ERMS 10; p(1) = 0.1) and metastasis formation (ARMS 12, ERMS 0; p(1) = 0.1). These properties also correlate with uniform greater overexpression of c-myc in ARMS (mean 39.3-fold. range 16-83) compared with ERMS (mean 5.3, range 4-8) (p(1) = 0.05, control fibroblasts = 1). Although c-myc was often amplified in vitro (four of six lines), there was no correlation with histologic type (2/3 ARMS, 2/3 ERMS). These data on rhabdomyosarcoma cell lines derived from verified ERMS and ARMS tumors support the impression from previous clinicopathologic observations that ARMS is a more malignant form of rhabdomyosarcoma than ERMS. C1 NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. NIH, WG Magnuson Clin Ctr, Bethesda, MD 20892 USA. RP Kouraklis, G (reprint author), Athens Univ Hlth Sci, 122 Vasilisis Sofias Ave, Athens 11526, Greece. NR 49 TC 16 Z9 16 U1 0 U2 0 PU INT PEDIATRIC RESEARCH FOUNDATION, INC PI BALTIMORE PA 351 WEST CAMDEN ST, BALTIMORE, MD 21201-2436 USA SN 0031-3998 J9 PEDIATR RES JI Pediatr. Res. PD APR PY 1999 VL 45 IS 4 BP 552 EP 558 DI 10.1203/00006450-199904010-00015 PN 1 PG 7 WC Pediatrics SC Pediatrics GA 182AK UT WOS:000079476200015 PM 10203148 ER PT J AU Kline, MW Blanchard, S Fletcher, CV Shenep, JL McKinney, RE Brundage, RC Culnane, M Van Dyke, RB Dankner, WM Kovacs, A McDowell, JA Hetherington, S AF Kline, MW Blanchard, S Fletcher, CV Shenep, JL McKinney, RE Brundage, RC Culnane, M Van Dyke, RB Dankner, WM Kovacs, A McDowell, JA Hetherington, S CA AIDS Clin Trials Grp 330 Team TI A phase I study of abacavir (1592U89) alone and in combination with other antiretroviral agents in infants and children with human immunodeficiency virus infection SO PEDIATRICS LA English DT Article DE abacavir; HIV infection; infant; child ID POTENT AB Objectives. To evaluate the pharmacokinetic features, safety, and tolerance of abacavir, given alone and in combination with other nucleoside antiretroviral agents, in symptomatic human immunodeficiency virus (HIV)-infected children. Methods. HIV-infected children discontinued prior antiretroviral therapy and were given abacavir orally, 4 mg/kg every 12 hours for 6 weeks, followed by 8 mg/kg every 12 hours for 6 weeks (n = 39); or 8 mg/kg every 12 hours for 12 weeks (n = 8). Children then were randomized to receive a second nucleoside antiretroviral agent (zidovudine, stavudine, didanosine, or lamivudine), plus abacavir. Pharmacokinetics, safety, tolerance, CD4(+) lymphocyte counts, and plasma HIV RNA concentrations were evaluated. Results. At a dose of 8 mg/kg every 12 hours, area under the plasma concentration-versus-time curves and plasma half-life values were comparable with those reported for adults receiving abacavir at a dose of 300 mg twice daily. One case each of hypersensitivity reaction and peripheral neuropathy occurred during abacavir monotherapy. Three children experienced neutropenia while receiving abacavir in combination with another antiretroviral agent. Mean CD4(+) lymphocyte count and plasma HIV RNA concentration did not change when prior antiretroviral therapy was changed to abacavir monotherapy. Conclusions. Abacavir therapy is associated with good short-term tolerance and safety in HIV-infected children. Phase III studies are in progress to assess the antiviral activity of abacavir in children and adults. C1 Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. Texas Childrens Hosp, Houston, TX 77030 USA. Harvard Univ, Sch Publ Hlth, Ctr Biostat AIDS Res, Boston, MA 02115 USA. Univ Minnesota, Coll Pharm, Minneapolis, MN 55455 USA. St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA. Duke Univ, Med Ctr, Dept Pediat, Durham, NC 27710 USA. NIAID, Div Aids, Bethesda, MD 20892 USA. Tulane Univ, Sch Med, Dept Pediat, New Orleans, LA 70112 USA. Univ Calif San Diego, Sch Med, Dept Pediat, San Diego, CA 92103 USA. Glaxo Wellcome Inc, Res Triangle Pk, NC USA. RP Kline, MW (reprint author), Baylor Coll Med, Dept Pediat, 1 Baylor Plaza, Houston, TX 77030 USA. NR 14 TC 25 Z9 25 U1 0 U2 4 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 J9 PEDIATRICS JI Pediatrics PD APR PY 1999 VL 103 IS 4 AR e47 DI 10.1542/peds.103.4.e47 PG 5 WC Pediatrics SC Pediatrics GA 182TZ UT WOS:000079515400025 PM 10103339 ER PT J AU Katoh, T Kaneko, S Takasawa, S Nagata, N Inatomi, H Ikemura, K Itoh, H Matsumoto, T Kawamoto, T Bell, DA AF Katoh, T Kaneko, S Takasawa, S Nagata, N Inatomi, H Ikemura, K Itoh, H Matsumoto, T Kawamoto, T Bell, DA TI Human glutathione S-transferase P1 polymorphism and susceptibility to smoking related epithelial cancer; oral, lung, gastric, colorectal and urothelial cancer SO PHARMACOGENETICS LA English DT Article DE cancer susceptibility; glutathione S-transferase P1-1; polymorphism; oral cancer ID SUPERGENE FAMILY; RISK; EPIDEMIOLOGY; EXPRESSION; GENOTYPES; JAPANESE; TOBACCO; TISSUE; MEN AB The A/G polymorphism at nucleotide 313 in the glutathione S-transferase P1-1 (GSTP1) gene was examined in patients with different types of smoking-related cancers (oral, lung, gastric, colorectal and urothelial cancers) and healthy control individuals. This polymorphism results in an amino acid substitution from isoleucine to valine at residue 105, which reduces catalytic activity of the enzyme. In control individuals, 23.8% of individuals had GSTP1 AG or GG genotype. This rose to 37.3% [n = 83, odds ratio = 1.93 (1.05-3.58), P = 0.035] in oral cancer patients. No increase in the frequency of the GSTP1 AG or GG genotype was obtained in lung, gastric, colorectal or urothelial cancers in this Japanese population. After grouping by smoking status, no consistent difference was observed between smoking patients and corresponding control individuals for the frequency of the GSTP1 A/G polymorphism for any cancer. However, a moderate risk (odds ratio = 2.78; 95% confidence interval 1.06-7.51) was associated with this polymorphism in the non-smoking group of oral cancer patients. The results suggest the GSTP1 polymorphism at nucleotide 313 may be associated with susceptibility to oral squamous cell carcinoma in the Japanese population. Pharmacogenetics 9:165-169 (C) 1999 Lippincott Williams & Wilkins. C1 Univ Occupat & Environm Hlth, Sch Hlth Sci, Dept Hlth Informat Sci, Kitakyushu, Fukuoka 807, Japan. Natl Inst Environm Hlth Sci, Lab Computat Biol & Risk Assessment, Res Triangle Pk, NC USA. Univ Occupat & Environm Hlth, Sch Med, Dept Oral Surg, Kitakyushu, Fukuoka 807, Japan. Univ Occupat & Environm Hlth, Sch Med, Dept Surg 1, Kitakyushu, Fukuoka 807, Japan. Univ Occupat & Environm Hlth, Sch Med, Dept Urol, Kitakyushu, Fukuoka 807, Japan. Univ Occupat & Environm Hlth, Sch Med, Dept Environm Hlth, Kitakyushu, Fukuoka 807, Japan. RP Katoh, T (reprint author), Univ Occupat & Environm Hlth, Sch Hlth Sci, Dept Hlth Informat Sci, Kitakyushu, Fukuoka 807, Japan. NR 19 TC 95 Z9 107 U1 1 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0960-314X J9 PHARMACOGENETICS JI Pharmacogenetics PD APR PY 1999 VL 9 IS 2 BP 165 EP 169 PG 5 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA 216CB UT WOS:000081422000004 PM 10376763 ER PT J AU Cravchik, A Gejman, PV AF Cravchik, A Gejman, PV TI Functional analysis of the human D-5 dopamine receptor missense and nonsense variants: differences in dopamine binding affinities SO PHARMACOGENETICS LA English DT Article DE dopamine; neuroleptics; schizophrenia; affinity ID MICROSATELLITE POLYMORPHISM; AMINO-ACID; GENE DRD1; SCHIZOPHRENIA; D1; IDENTIFICATION; ASSOCIATION; MUTAGENESIS; INHIBITION; MUTATION AB The functional analysis of expressed human gene variants is important in the study of genetic susceptibility to diseases, pharmacogenetic traits and for the investigation of the human genetic diversity at the molecular level. We have performed the analysis of sequence polymorphisms in the human D-5 dopamine receptor gene (DRD5) predicting missense and nonsense amino acid changes in the receptor protein. The amino acid substitutions in the human D-5 dopamine receptor are: Leu(88) to Phe in the putative second transmembrane domain, Ala(269) to Val in the third intracellular and Pro(330) to Gln in the third extracellular loops, Asn(351) to Asp in the seventh transmembrane and Ser(453) to Cys in the C-terminal domains and Cys(335) to Stop in the third extracellular loop. The two amino acid substitutions in the transmembrane domains had an effect on agonist binding to the human D-5 dopamine receptor. Asn(351) to Asp resulted in an approximately 10-fold decrease in dopamine and threefold decrease in R(+)-SKF-38393 binding affinities. Leu(88) to Phe resulted in a small increase in dopamine binding affinity. Antagonist binding affinities were mostly unaffected by the amino acid substitutions with the exception of Leu(88) to Phe, which showed small reductions in binding affinities of SCH-23390 and risperidone. The existence of functionally different variants of the human dopamine receptors might have phenotypic consequences given their importance in central nervous system physiology and pharmacology. Pharmacogenetics 9:199-206 (C) 1999 Lippincott Williams & Wilkins. C1 NIAAA, Neurogenet Lab, Bethesda, MD USA. Univ Chicago, Dept Psychiat, Chicago, IL 60637 USA. RP Cravchik, A (reprint author), Celera Genom Corp, 45 W Gude Dr, Rockville, MD 20850 USA. NR 34 TC 43 Z9 47 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0960-314X J9 PHARMACOGENETICS JI Pharmacogenetics PD APR PY 1999 VL 9 IS 2 BP 199 EP 206 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA 216CB UT WOS:000081422000008 PM 10376767 ER PT J AU Sai, Y Yang, TJ Krausz, KW Gonzalez, FJ Gelboin, HV AF Sai, Y Yang, TJ Krausz, KW Gonzalez, FJ Gelboin, HV TI An inhibitory monoclonal antibody to human cytochrome P450 2A6 defines its role in the metabolism of coumarin, 7-ethoxycoumarin and 4-nitroanisole in human liver SO PHARMACOGENETICS LA English DT Article DE human CYP2A6; inhibitory monoclonal antibody; coumarin; 7-ethoxycoumarin; phenanthrene; 4-nitroanisole; 4-nitrophenol; human liver microsomes ID CDNA-EXPRESSED HUMAN; HUMAN CYP2A6; NITROPHENOL HYDROXYLATION; SPECIES-DIFFERENCES; MICROSOMES; ENZYMES; ACTIVATION; 2E1; RAT; 7-HYDROXYLATION AB Cytochrome P450 (CYP) 2A6 is an important enzyme catalysing the metabolism of many drugs, procarcinogens and promutagens. Its role in human liver metabolism of coumarin, 4-nitroanisole, 4nidrophenol and 7-ethoxycoumarin was analysed with an inhibitory monoclonal antibody (MAb) to CYP2A6. MAbs were derived from a panel of 16 hybridomas which yielded positive enzyme-linked immunosorbent assay (ELISA) results or Immunoblots against CYP2A6. The hybridomas were selected from more than 500 clones generated by the fusion of myeloma cells with spleen cells of mice immunized with purified baculovirus-expressed human CYP2A6. The MAbs obtained from four of the 16 hybridomas exhibited strong inhibitory activity to CYP2A6-catalysed phenanthrene metabolism. MAb 151-45-4 was positive and highly specific to CYP2A6 as determined by ELISA and immunoblot, and showed no cross-reactivity with recombinant human CYP 1A1, 1A2, 2B6, 2C8, 2C9, 2C19, 2D6, 2E1, 3A4 and 3A5, as tested with ELISA and immunoblot analyses. MAb 151-45-4 specifically inhibited CYP2A6-catalysed metabolism of phenanthrene, 4-nitroanisole, 4-nitrophenol, coumarin and 7-ethoxycoumarin each by 94-99% and did not inhibit their metabolism catalysed: by 10 other human CYPs. The potent inhibitory effect of MAb 151-45-4 was used to define the contribution of human CYP2A6 to the metabolism of coumarin, 4-nitroanisole and 7-ethoxycoumarin in seven human liver microsome samples. Coumarin metabolism in all of the seven samples was inhibited by greater than 94% by MAb 151-45-4 which indicates that essentially all microsome mediated coumarin metabolism in human liver is catalysed only by CYP2A6. Inhibition of 4-nitroanisole and 7-ethoxycoumarin metabolism by anti 2A6 MAb ranged from 22-65% and 8-24%, respectively. The degree of inhibition defines the contribution of CYP2A6 activity to the 4-nitroanisole and 7-ethoxycoumarin metabolism in human liver and the range reflects the variability among samples. The inhibitory antibody to CYP2E1 was used to determine its role in 4-nitroanisole and 7-ethoxycoumarin metabolism in seven human liver samples. The addition of both MAbs to CYP2A6 and 2E1 to the microsome samples defined combinatorially the relative role of CYP2A6 and 2E1 in the metabolism of 4-nitroanisole and 7-ethoxycoumarin. Pharmacogenetics 9:229-237 (C) 1999 Lippincott Williams & Wilkins. C1 NCI, Lab Metab, NIH, Bethesda, MD 20892 USA. NCI, Mol Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. RP Gelboin, HV (reprint author), NCI, Lab Metab, NIH, Bethesda, MD 20892 USA. NR 43 TC 17 Z9 18 U1 0 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0960-314X J9 PHARMACOGENETICS JI Pharmacogenetics PD APR PY 1999 VL 9 IS 2 BP 229 EP 237 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Genetics & Heredity; Pharmacology & Pharmacy GA 216CB UT WOS:000081422000011 PM 10376770 ER PT J AU Trier, U Olah, Z Kleuser, B Schafer-Korting, M AF Trier, U Olah, Z Kleuser, B Schafer-Korting, M TI Fusion of the binding domain of Raf-1 kinase with green fluorescent protein for activated Ras detection by fluorescence correlation spectroscopy SO PHARMAZIE LA English DT Article ID ROTATIONAL DIFFUSION; IN-VIVO; CELLS; IDENTIFICATION; FLUCTUATIONS; LOCALIZATION; GTPASE; DNA; EXPRESSION; ONCOGENES AB Rns proto-oncogenes play a central role in cell proliferation by the regulation of signal transduction pathways from receptors of the outer cell membrane to the nucleus via the activation of transcription factors. Wild-type Ras cycles between the activated GTP-bound and the inactivated GDP-bound state, and the GTPase reaction is a timer for the interaction between Ras-GTP and effector molecules such as Raf-l protein kinase. Mutations of,ras resulting in the loss of the intrinsic GTPase activity result in autonomous proliferation. Mutated Ras is found in a variety of human tumors. Therefore, monitoring of GTP-loaded conformation of Ras related proteins could be utilised in cancer diagnosis. To develop a fluorescence based bioassay we have coupled the gene for the N-terminal Ras binding domain (RBD) of Raf-l protein kinase with the gene for the green fluorescent protein (GFP). The chimeric fusion protein RBDGFP was identified by immunoblotting and subsequently investigated by fluorescence correlation spectroscopy (FCS), a new analytical technology allowing the measurement of characteristic diffusion times of fluorescently labeled molecules. Molecular interactions increase the molecular weight and influence the diffusion time of RBDGFP. FCS diffusion value of the recombinant protein was in coincidence with the molecular weight of the construct. Fluorimetric measurements of RBDGFP versus GFP showed clearly that the recombinant protein contains functional GFP. Increased FCS transition times indicated the interaction of RBDGFP with its corresponding antibody. Suboptimal binding of the fusion protein to activated Ras, however, resulted in a modest influence on the diffusion value. Taken together our rational design and construct shows the way for a ready characterisation of novel GFP-connected fusion proteins employing FCS. C1 Free Univ Berlin, Inst Pharm 2, D-14195 Berlin, Germany. Natl Inst Hlth, Natl Canc Inst, Bethesda, MD USA. RP Schafer-Korting, M (reprint author), Free Univ Berlin, Inst Pharm 2, Konigin Luise Str 2-4, D-14195 Berlin, Germany. NR 54 TC 12 Z9 12 U1 0 U2 1 PU GOVI-VERLAG GMBH PI ESCHBORN PA PHARMAZEUTISCHER VERLAG GINNHEIMER STRASSE 26, D-65760 ESCHBORN, GERMANY SN 0031-7144 J9 PHARMAZIE JI Pharmazie PD APR PY 1999 VL 54 IS 4 BP 263 EP 268 PG 6 WC Chemistry, Medicinal; Chemistry, Multidisciplinary; Pharmacology & Pharmacy SC Pharmacology & Pharmacy; Chemistry GA 189YM UT WOS:000079934000007 PM 10234739 ER PT J AU Finkelstein, JA Schiffman, SS AF Finkelstein, JA Schiffman, SS TI Workshop on taste and smell in the elderly: An overview SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE aging; taste; smell; olfaction; nutrition ID PERCEPTION; NUTRITION; DISEASE; RISK AB The purpose of the workshop entitled Taste and Smell in the Elderly: Behavioral and Nutritional Consequences was 1) to review the current state of knowledge in the area of taste and smell, with emphasis on age-related changes, 2) to identify existing gaps in our knowledge, and 3) to develop future research strategies. There was general agreement that the majority of scientific studies have found impairments in taste and smell acuity in the elderly. These losses may result from normal aging, certain disease states especially Alzheimer's disease, medications, surgical interventions, and environmental exposure. However, there are gaps in our knowledge of the basic mechanisms by which aging and environmental factors may impair the chemical senses in the elderly. Further research is also required in a variety of areas including chemosensory test procedures, food intake, and nutrition to understand fully the impact of chemosensory dysfunction on older individuals. (C) 1999 Elsevier Science Inc. C1 NIA, Sensory Motor Disorders Aging Program, Bethesda, MD 20892 USA. Duke Univ, Sch Med, Dept Psychiat, Durham, NC 27710 USA. RP Finkelstein, JA (reprint author), NIA, Sensory Motor Disorders Aging Program, Gateway Bldg,Suite 3C307, Bethesda, MD 20892 USA. NR 20 TC 19 Z9 20 U1 3 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD APR PY 1999 VL 66 IS 2 BP 173 EP 176 DI 10.1016/S0031-9384(98)00261-3 PG 4 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA 192ZC UT WOS:000080108800001 PM 10336140 ER PT J AU Hou, L AF Hou, L TI Effects of local tissue environment on the differentiation of neural crest cells in turtle, with special reference to understanding the spatial distribution of pigment cells SO PIGMENT CELL RESEARCH LA English DT Article DE neural crest; melanophore differentiation; melanization stimulating factor; reptiles ID MONOCLONAL-ANTIBODY; PATTERN-FORMATION; MIGRATION; MELANOCYTES; EMBRYOS; SKIN; MELANIZATION; SEGREGATION; MESENCHYME; INVITRO AB The spatial distribution of neural crest-derived pigment cells in turtles differs markedly from those found in chickens and mice, One hypothesis to explain such differences in the spatial distribution of pigment cells is that local tissue factors interact with neural crest cells, thereby determining their differentiation into pigment-synthesizing cells. It is reported here that local tissue factors in the soft-shell turtle (Trionyx sinensis japonicus) play a critical role in the development of melanophores from neural crest cells during embryogenesis, Undifferentiated neural crest cells derived from trunk neural tubes were co-cultured in vitro with homochronous somites, or with heterochronous dermis, lung or liver for 14 days, Melanophore differentiation from neural crest cells was significantly promoted when co-cultured with cells from lung, somites or dermis, but not when co-cultured with liver cells, These results suggest that local tissue factors stimulate the differentiation of pluripotent neural crest derivatives toward pigment cells, It is proposed that specific environmental cues play an important role in the spatial distribution of pigment cells in a variety of vertebrate species. C1 Tohoku Univ, Inst Biol, Sendai, Miyagi 980, Japan. RP Hou, L (reprint author), NIH, Bldg 36-5D04,36 Convent Dr MSC 4160, Bethesda, MD 20892 USA. OI Hou, Ling/0000-0003-0705-8099 NR 34 TC 9 Z9 10 U1 0 U2 1 PU MUNKSGAARD INT PUBL LTD PI COPENHAGEN PA 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK SN 0893-5785 J9 PIGM CELL RES JI Pigm. Cell. Res. PD APR PY 1999 VL 12 IS 2 BP 81 EP 88 DI 10.1111/j.1600-0749.1999.tb00747.x PG 8 WC Cell Biology; Dermatology SC Cell Biology; Dermatology GA 189KK UT WOS:000079903500003 PM 10231195 ER PT J AU Spong, CY Ghidini, A Stanley-Christian, H Meck, JM Seydel, FD Pezzullo, JC AF Spong, CY Ghidini, A Stanley-Christian, H Meck, JM Seydel, FD Pezzullo, JC TI Risk of abnormal triple screen for Down syndrome is significantly higher in patients with female fetuses SO PRENATAL DIAGNOSIS LA English DT Article DE prenatal diagnosis; trisomy 21; fetal gender ID SERUM ALPHA-FETOPROTEIN; FETAL SEX; GENDER AB Previous studies have shown that mid-trimester maternal serum alpha-fetoprotein (AFP) levels are significantly higher and human chorionic gonadotrophin (hCG) levels significantly lower in women with male compared with female fetuses. We have evaluated whether triple-screen criteria are more likely to identify women with female fetuses as at risk for Down syndrome. From the Georgetown University genetics database we obtained the absolute values and corresponding multiples of the median (MoM) for AFP, hCG and unconjugated oestriol (uE3) in singleton gestations for the period database November 1992-July 1996. A Down syndrome risk of 1/270 or greater at mid-trimester was considered as high risk. A total of 977 patients with triple screen and outcome information were identified, including 502 female and 475 male fetuses. Patients with female fetuses were significantly more likely to have lower serum AFP (p=0.003) and a positive triple screen for Down syndrome (72 (14 per cent) versus 45 (9 per cent), p<0.02) than those with male fetuses. The gestational age at triple screen, maternal serum hCG and uE3, race and diabetes were not significantly different between the two groups. Since Down syndrome is less common in female than male fetuses, and the rates of female and male Down syndrome fetuses detected by triple screen and subsequent amniocentesis are not significantly different, the excess of positive mid-trimester maternal serum triple screen in women with female fetuses is likely due to false-positive results. Copyright (C) 1999 John Wiley & Sons, Ltd. C1 Georgetown Univ, Med Ctr, Dept Obstet & Gynecol, Washington, DC 20007 USA. NICHD, Dev Neurobiol Lab, NIH, Bethesda, MD USA. RP Ghidini, A (reprint author), Georgetown Univ, Med Ctr, Dept Obstet & Gynecol, 3PHC,3800 Reservoir Rd NW, Washington, DC 20007 USA. NR 8 TC 15 Z9 15 U1 0 U2 2 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX PO19 1UD, ENGLAND SN 0197-3851 J9 PRENATAL DIAG JI Prenat. Diagn. PD APR PY 1999 VL 19 IS 4 BP 337 EP 339 DI 10.1002/(SICI)1097-0223(199904)19:4<337::AID-PD553>3.0.CO;2-4 PG 3 WC Genetics & Heredity; Obstetrics & Gynecology SC Genetics & Heredity; Obstetrics & Gynecology GA 191MC UT WOS:000080025300008 PM 10327139 ER PT J AU Salive, ME Stein, DH AF Salive, ME Stein, DH TI Predictors and correlates of prevention research careers: A national study of residency graduates SO PREVENTIVE MEDICINE LA English DT Article; Proceedings Paper CT 124th Annual Meeting of the American-Public-Health-Association CY NOV 17-21, 1996 CL NEW YORK, NEW YORK SP Amer Public Hlth Assoc DE physician professional practice; preventive medicine; education, medical; residency training; clinical research ID PUBLIC-HEALTH; MEDICINE; REFORM AB Background Factors associated with research productivity among residency graduates are not well understood, The objectives of this study are to describe research productivity among preventive medicine residency (PMR) graduates and to identify factors that are correlated with high levels of productivity. Methods. A detailed survey was mailed to all (n = 1,070) graduates from U.S. PMRs between 1979 and 1989, Main outcome measures for this analysis were (1) 25% of the workweek or more research time and (2) 20 or more publications since training completion. Results. A total of 797 completed surveys were received for a response rate of 75%. Among respondents, 33% devoted at least 25% of their time to research and 13% had 20 or more publications. Independent positive predictors (P < 0.05) based on education and training of high research productivity as measured by both outcomes included research self-motivation, training at the Centers for Disease Control and Prevention, and clinical board certification. Concurrent correlates of current high research productivity by both outcomes included employment by the federal government or academia and academic appointment. Conclusions, Factors associated with high research productivity could be utilized to improve the resident selection process and promote research careers. This could enhance research programs and education and promote the overall prevention research agenda. (C) 1999 American Health Foundation and Academic Press. C1 NIA, Epidemiol Demog & Biometry Program, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Hlth Policy & Management, Baltimore, MD 21205 USA. Johns Hopkins Univ, Sch Med, Dept Dermatol, Baltimore, MD 21205 USA. RP Salive, ME (reprint author), US FDA, Epidemiol Branch, Ctr Biol Evaluat & Res, 1401 Rockville Pike,HFM-220, Rockville, MD 20852 USA. NR 23 TC 4 Z9 4 U1 0 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0091-7435 J9 PREV MED JI Prev. Med. PD APR PY 1999 VL 28 IS 4 BP 430 EP 436 DI 10.1006/pmed.1998.0449 PG 7 WC Public, Environmental & Occupational Health; Medicine, General & Internal SC Public, Environmental & Occupational Health; General & Internal Medicine GA 183MF UT WOS:000079556800013 PM 10090873 ER PT J AU Chow, WH Blot, WJ McLaughlin, JK AF Chow, WH Blot, WJ McLaughlin, JK TI Tea drinking and cancer risk: Epidemiologic evidence SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Editorial Material C1 NCI, Div Canc Epidemiol, Bethesda, MD 20892 USA. International Epidemiol Inst, Rockville, MD 20852 USA. RP Chow, WH (reprint author), NCI, Div Canc Epidemiol, Bethesda, MD 20892 USA. NR 4 TC 5 Z9 5 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD APR PY 1999 VL 220 IS 4 BP 197 EP 197 DI 10.1046/j.1525-1373.1999.d01-32.x PG 1 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 179JR UT WOS:000079324000018 ER PT J AU Steele, VE Bagheri, D Balentine, DA Boone, CW Mehta, R Morse, MA Sharma, S Sigman, CC Stoner, GD Wargovich, MJ Weisburger, JH Zhu, SY Kelloff, GJ AF Steele, VE Bagheri, D Balentine, DA Boone, CW Mehta, R Morse, MA Sharma, S Sigman, CC Stoner, GD Wargovich, MJ Weisburger, JH Zhu, SY Kelloff, GJ TI Preclinical efficacy studies of green and black tea extracts SO PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE LA English DT Article; Proceedings Paper CT 2nd International Scientific Symposium on Tea and Human Health CY SEP 14-15, 1998 CL WASHINGTON, D.C. SP Amer Canc Soc C1 NCI, Chemoprevent Branch, Bethesda, MD 20892 USA. ManTech Environm Technol Inc, Res Triangle Pk, NC 27709 USA. Univ Illinois, Chicago, IL 60612 USA. Univ Texas, MD Anderson Canc Ctr, Houston, TX 77030 USA. Thomas J Lipton Co, Englewood Cliffs, NJ 07632 USA. CCS Associates, Mt View, CA 94043 USA. Ohio State Univ, Columbus, OH 43210 USA. Amer Hlth Fdn, Valhalla, NY 10595 USA. RP Steele, VE (reprint author), NCI, Chemoprevent Branch, 9000 Rockville Pike,Execut Plaza N,Suite 201, Bethesda, MD 20892 USA. FU NCI NIH HHS [N01-CN-25466-05, N01-CN-25466-08] NR 5 TC 29 Z9 33 U1 0 U2 0 PU BLACKWELL SCIENCE INC PI MALDEN PA 350 MAIN ST, MALDEN, MA 02148 USA SN 0037-9727 J9 P SOC EXP BIOL MED JI Proc. Soc. Exp. Biol. Med. PD APR PY 1999 VL 220 IS 4 BP 210 EP 212 DI 10.1046/j.1525-1373.1999.d01-35.x PG 3 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA 179JR UT WOS:000079324000005 PM 10202390 ER PT J AU Oriji, GK AF Oriji, GK TI Endothelin-induced prostacyclin production in rat aortic endothelial cells is mediated by protein kinase C SO PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS LA English DT Article ID PIGLET PARIETAL CORTEX; SMOOTH-MUSCLE CELLS; PROSTAGLANDIN-E2 PRODUCTION; SIGNAL TRANSDUCTION; PLATELET ACTIVATION; MESANGIAL CELLS; PROSTANOIDS; PHORBOL; RELEASE; PHOSPHORYLATION AB Endothelin (ET) is a vasoconstrictor peptide released from endothelial cells that is known to cause prostaglandin (PG) release. The mechanism remains unclear. To determine whether the protein kinase C (PKC) signaling pathway is stimulated by endothelin, we pretreated rat aortic endothelial cells with either PKC activator or inhibitors and measured the release of prostacyclin (PGI(2)) by radioimmunoassay. ET (10(-9) M) produced a 10-fold increase in PGI(2) release. Pretreatment with 10(-9) M of three different PKC inhibitors: 1-(5-isoquinolinesulfonyl) piperazine (CL), staurosporine, and 1-(5-isoquinolinesulfonyl-methyl) piperazine (H7) blocked ET induced PGI(2) release. ET induced prostacyclin release was also blocked by pretreatment with inhibitors of either phospholipase A(2) (7,7,dimethyleicosadienoic acid or trifluoromethyl ketone analogue) (10-9 M) or cyclooxygenase (indomethacin) (10(-9)M). We conclude that ET activates PKC which activates phospholipase A(2) which liberates arachidonic acid which increases PGI, production and release. C1 William Paterson Univ, Coll Sci & Hlth, Dept Biol, Wayne, NJ 07470 USA. NHLBI, Hypertens Endocrine Branch, NIH, Bethesda, MD 20892 USA. RP Oriji, GK (reprint author), William Paterson Univ, Coll Sci & Hlth, Dept Biol, Wayne, NJ 07470 USA. NR 39 TC 5 Z9 5 U1 0 U2 0 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0952-3278 J9 PROSTAG LEUKOTR ESS JI Prostaglandins Leukot. Essent. Fatty Acids PD APR PY 1999 VL 60 IS 4 BP 263 EP 268 DI 10.1054/plef.1999.0034 PG 6 WC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Cell Biology; Endocrinology & Metabolism GA 207XY UT WOS:000080962100006 PM 10397408 ER PT J AU Drohat, AC Tjandra, N Baldisseri, DM Weber, DJ AF Drohat, AC Tjandra, N Baldisseri, DM Weber, DJ TI The use of dipolar couplings for determining the solution structure of rat apo-S100B(beta beta) SO PROTEIN SCIENCE LA English DT Article DE Ca2+-binding protein; dipolar coupling; EF-hands; NMR; S100 proteins; S100 beta; S100B; three-dimensional structure ID PROTEIN-KINASE-C; NUCLEAR-MAGNETIC-RESONANCE; CALCIUM-BINDING PROTEINS; SECONDARY STRUCTURE; NMR-SPECTROSCOPY; ORIENTED MACROMOLECULES; 3-DIMENSIONAL STRUCTURE; CA2+-DEPENDENT MANNER; HUMAN UBIQUITIN; S-100 PROTEIN AB The relative orientations of adjacent structural elements without many well-defined NOE contacts between them are typically poorly defined in NMR structures. For apo-S100B(PP) and the structurally homologous protein calcyclin, the solution structures determined by conventional NMR exhibited considerable differences and made it impossible to draw unambiguous conclusions regarding the Ca2+-induced conformational change required for target protein binding. The structure of rat apo-S100B(PP) was recalculated using a large number of constraints derived from dipolar couplings that were measured in a dilute liquid crystalline phase. The dipolar couplings orient bond vectors relative to a single-axis system, and thereby remove much of the uncertainty in NOE-based structures. The structure of apo-S100B(PP) indicates a minimal change in the first, pseudo-EF-hand Ca2+ binding site, but a large reorientation of helix 3 in the second, classical EF-hand upon Ca2+ binding. C1 Univ Maryland, Sch Med, Dept Biochem & Mol Biol, Baltimore, MD 21201 USA. NHLBI, Biophys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Weber, DJ (reprint author), Univ Maryland, Sch Med, Dept Biochem & Mol Biol, 108 N Greene St, Baltimore, MD 21201 USA. FU NCRR NIH HHS [S10RR10441]; NIGMS NIH HHS [R01 GM058888, R01GM58888] NR 54 TC 82 Z9 85 U1 0 U2 0 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 40 WEST 20TH STREET, NEW YORK, NY 10011-4211 USA SN 0961-8368 J9 PROTEIN SCI JI Protein Sci. PD APR PY 1999 VL 8 IS 4 BP 800 EP 809 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 184EE UT WOS:000079596800011 PM 10211826 ER PT J AU Matsuo, Y Bryant, SH AF Matsuo, Y Bryant, SH TI Identification of homologous core structures SO PROTEINS-STRUCTURE FUNCTION AND GENETICS LA English DT Article DE protein structure; comparative analysis; molecular evolution ID DATABASE; SEQUENCE; DOMAIN; CLASSIFICATION; RECOGNITION; PROTEINS; MOTIFS; ENTREZ; FOLDS AB Using a large database of protein structure-structure alignments, we test a new method for distinguishing homologous and "analogous" structural neighbors. The homologous neighbors included in the test set show no detectable sequence similarity but they may be well superimposed and show functional similarity or other evidence of evolutionary relationship. Analogous neighbors also show no sequence similarity and may be well superimposed, but they have different functions and their structural similarity may be the result of convergent evolution. Confirming results of other analyses, we find that remote homologs and analogs are not well distinguished by measures of pairwise structural similarity including the percentage of identical residues and root-mean-square (RMS) superposition residual. We show, however, that with structure-structure alignments of analogous neighbors rarely superimpose the particular substructure that is shared among homologous neighbors. We call this characteristic substructure the homologous core structure (HCS), and we show that a cross-validated test for presence of the HCS correctly identifies 75% of remote homologs with a false-positive rate of 16% analogs, significantly better than discrimination by RMS or other measures of pairwise similarity The HCS describes conservation of spatial structure within a protein family in much the way that a sequence motif describes sequence conservation. We suggest that it may be used in the same way, to identify homologous neighbors at greater evolutionary distance than is possible by pairwise comparison. Proteins 1999;35:70-79. Published 1999 Wiley-Liss, Inc. C1 NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. RP Bryant, SH (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, 8600 Rockville Pike, Bethesda, MD 20894 USA. NR 29 TC 54 Z9 54 U1 0 U2 1 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0887-3585 J9 PROTEINS JI Proteins PD APR 1 PY 1999 VL 35 IS 1 BP 70 EP 79 DI 10.1002/(SICI)1097-0134(19990401)35:1<70::AID-PROT7>3.0.CO;2-9 PG 10 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 173NZ UT WOS:000078987800007 PM 10090287 ER PT J AU Benjamin, J Geraci, M McCann, U Greenberg, BD Murphy, DL AF Benjamin, J Geraci, M McCann, U Greenberg, BD Murphy, DL TI Attenuated response to m-CPP and to pentagastrin after repeated m-CPP in panic disorder SO PSYCHOPHARMACOLOGY LA English DT Letter ID CHOLECYSTOKININ-TETRAPEPTIDE; CHLOROPHENYLPIPERAZINE; TEMPERATURE; VOLUNTEERS; CHALLENGE; RECEPTOR C1 Soroka Med Ctr, Dept Psychiat, IL-84101 Beer Sheva, Israel. NIMH, Clin Sci Lab, Bethesda, MD 20892 USA. NIMH, Biol Psychiat Branch, Bethesda, MD 20892 USA. RP Benjamin, J (reprint author), Soroka Med Ctr, Dept Psychiat, POB 151, IL-84101 Beer Sheva, Israel. NR 11 TC 12 Z9 12 U1 0 U2 0 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 1999 VL 143 IS 2 BP 215 EP 216 DI 10.1007/s002130050938 PG 2 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 188TM UT WOS:000079864600013 PM 10326785 ER PT J AU Munzar, P Goldberg, SR AF Munzar, P Goldberg, SR TI Noradrenergic modulation of the discriminative-stimulus effects of methamphetamine in rats SO PSYCHOPHARMACOLOGY LA English DT Article DE methamphetamine; drug discrimination; norepinephrine; desipramine; nisoxetine; isoproterenol; propranolol; methoxamine; prazosin; clonidine; yohimbine; rat ID VENTRAL TEGMENTAL AREA; PREFRONTAL CORTEX; SPONTANEOUS RECURRENCE; SQUIRREL-MONKEYS; AMPHETAMINE; COCAINE; DOPAMINE; NOREPINEPHRINE; CLONIDINE; STEREOISOMERS AB Rationale: Neurochemical and clinical studies indicate involvement of noradrenergic (NE) neurotransmitter system in the actions of methamphetamine. Objective: The present study investigated NE involvement in the discriminative-stimulus effects of methamphetamine. Methods: In Sprague-Dawley rats trained to discriminate 1.0 mg/kg methamphetamine, IP, from saline under a fixed-ratio schedule of food presentation, effects of various NE agonists, antagonists and uptake inhibitors were tested. Results: Desipramine (3.0-18.0 mg/kg) and nisoxetine (5.6-30.0 mg/kg), two selective NE-uptake inhibitors, did not significantly generalize to methamphetamine when administered alone, but 5.6 mg/kg desipramine and 10.0 mg/kg nisoxetine significantly shifted the methamphetamine dose-response curve to the left. The beta NE agonist, isoproterenol (0.56-3.0 mg/kg), and antagonist, propranolol (1.0-18.0 mg/kg), neither generalized to methamphetamine when given alone nor altered the discriminative-stimulus effects of methamphetamine when administered in combination. The alpha-1 NE agonist methoxamine (1.0-5.6 mg/kg) failed to generalize to the methamphetamine training stimulus. When given in combination with methamphetamine, the alpha-1 NE antagonist, prazosin (1.0 mg/kg), shifted the methamphetamine dose-response curve somewhat to the right and partially blocked the discriminative-stimulus effects of the 1.0 mg/kg training dose of methamphetamine, but these changes were not significant or dose-related, with further increases in prazosin dose (1.8-10.0 mg/kg) either producing similar or smaller changes. The alpha-2 NE agonist, clonidine, partially generalized to methamphetamine at doses of 0.1-0.18 mg/kg and increased drug-appropriate responding at lower doses of methamphetamine, but it partially blocked the discriminative-stimulus effects of higher 0.56-1.0 mg/kg doses of methamphetamine over the same dose range. The alpha-2 NE antagonist, yohimbine, also partially generalized to methamphetamine and blocked the discriminative-stimulus effects of the 1.0 mg/kg training dose of methamphetamine at doses of 5.6-10.0 mg/kg. A lower 3.0 mg/kg dose of yohimbine increased methamphetamine-appropriate responding when given together with low 0.1-0.3 mg/kg doses of methamphetamine. Conclusions: The present data suggest that the NE system plays a modulatory role in the discriminative-stimulus effects of methamphetamine. These effects appear to be mediated through NE uptake sites and alpha-2 receptors, with limited involvement of alpha-1 receptors and beta receptors. C1 NIDA, Preclin Pharmacol Lab, NIH, Intramural Res Program, Baltimore, MD 21224 USA. RP Goldberg, SR (reprint author), NIDA, Preclin Pharmacol Lab, NIH, Intramural Res Program, 550 Nathan Shock Dr, Baltimore, MD 21224 USA. EM sgoldber@intra.nida.nih.gov NR 41 TC 34 Z9 34 U1 1 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 1999 VL 143 IS 3 BP 293 EP 301 DI 10.1007/s002130050950 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 192ZU UT WOS:000080110300012 PM 10353433 ER PT J AU McCann, UD Mertl, M Eligulashvili, V Ricaurte, GA AF McCann, UD Mertl, M Eligulashvili, V Ricaurte, GA TI Cognitive performance in (+/-) 3,4-methylenedioxymethamphetamine (MDMA, "ecstasy") users: a controlled study SO PSYCHOPHARMACOLOGY LA English DT Article DE serotonin; neurotoxicity; memory; attention; amphetamine ID CENTRAL SEROTONERGIC NEURONS; CEREBROSPINAL-FLUID; RAT-BRAIN; (+/-)3,4-METHYLENEDIOXYMETHAMPHETAMINE MDMA; METHYLENEDIOXYMETHAMPHETAMINE MDMA; RECREATIONAL USERS; SLEEP-DEPRIVATION; NONHUMAN-PRIMATES; NEUROTOXICITY; CATECHOLAMINES AB Rationale: (+) 3,4-Methylenedioxymethamphetamine (MDMA, "ecstasy") is an amphetamine analog and drug of abuse. In animals, MDMA damages brain serotonin (5-HT) neurons at doses that overlap with those used recreationally by some humans. To date, few functional sequelae of MDMA-induced 5-HT damage have been identified. Objective. Since serotonin is thought to be involved in cognitive processes, and since previous studies have reported verbal and visual memory deficits in MDMA users, the present study sought to determine whether other cognitive processes are influenced by previous exposure to MDMA. Methods: Twenty-two MDMA users who had not used MDMA for at least 3 weeks and 23 control subjects were tested repeatedly with a computerized cognitive performance assessment battery while participating in a 5-day controlled inpatient study. Cerebrospinal fluid (CSF) measures of monoamine metabolites were also collected as an index of brain monoaminergic function. Results: MDMA users and controls were found to perform similarly on several cognitive tasks. However, MDMA subjects had significant performance deficits on a sustained attention task requiring arithmetic calculations, a task requiring complex attention and incidental learning, a task requiring short term memory and a task of semantic recognition and verbal reasoning. MDMA users also had significant selective decreases in CSF 5-HIAA. Conclusions: The present CSF data provide further evidence that MDMA is neurotoxic to brain 5-HT neurons in humans, and the behavioral data suggest that brain 5-HT injury is associated with subtle, but significant, cognitive deficits. C1 NIMH, Unit Anxiety, Biol Psychiat Branch, Bethesda, MD 20892 USA. Johns Hopkins Med Inst, Dept Neurol, Baltimore, MD 21224 USA. RP Ricaurte, GA (reprint author), NIMH, Unit Anxiety, Biol Psychiat Branch, Bethesda, MD 20892 USA. FU NIDA NIH HHS [DA05938, DA00206, DA05707] NR 40 TC 186 Z9 190 U1 1 U2 8 PU SPRINGER VERLAG PI NEW YORK PA 175 FIFTH AVE, NEW YORK, NY 10010 USA SN 0033-3158 J9 PSYCHOPHARMACOLOGY JI Psychopharmacology PD APR PY 1999 VL 143 IS 4 BP 417 EP 425 DI 10.1007/s002130050967 PG 9 WC Neurosciences; Pharmacology & Pharmacy; Psychiatry SC Neurosciences & Neurology; Pharmacology & Pharmacy; Psychiatry GA 195WZ UT WOS:000080276300012 PM 10367560 ER PT J AU Caan, BJ Lanza, E Schatzkin, A Coates, AO Brewer, BK Slattery, ML Marshall, JR Bloch, A AF Caan, Bette J. Lanza, Elaine Schatzkin, Arthur Coates, Ashley O. Brewer, Brenda K. Slattery, Martha L. Marshall, James R. Bloch, Abby TI Does nutritionist review of a self-administered food frequency questionnaire improve data quality? SO PUBLIC HEALTH NUTRITION LA English DT Article DE Food frequency; Diet methods; Validation; Dietary intake AB Objective: This study sought to evaluate the benefit of utilizing a nutritionist review of a self-administered food frequency questionnaire (FFQ), to determine whether accuracy could be improved beyond that produced by the self-administered questionnaire alone. Design: Participants randomized into a dietary intervention trial completed both a FFQ and a 4-day food record (FR) at baseline before entry into the intervention. The FFQ was self-administered, photocopied and then reviewed by a nutritionist who used additional probes to help complete the questionnaire. Both the versions before nutritionist review and after nutritionist review -were individually compared on specific nutrients to the FR by means, correlations and per cent agreement into quintiles. Settings and subjects: Three hundred and twenty-four people, a subset of participants from the Polyp Prevention Trial - a randomized controlled trial examining the effect of a low-fat, high-fibre, high fruit and vegetable dietary pattern on the recurrence of adenomatous polyps - were recruited from clinical centres at the University of Utah, University of Buffalo, Memorial Sloan Kettering Cancer Center in New York and Kaiser Permanente Medical Program in Oakland. Results: Reviewing the FFQ increased correlations with the FR for every nutrient, and per cent agreement into quintiles for all nutrients except calcium. Energy was underestimated in both versions of the FFQ but to a lesser degree in the version with review. Conclusions: One must further evaluate whether the increases seen with nutritionist review of the FFQ will enhance our ability to predict diet-disease relationships and whether it is cost-effective when participant burden and money spent utilizing trained personnel are considered. C1 [Caan, Bette J.; Coates, Ashley O.] Kaiser Permanente, Med Care Program Northern Calif, Div Res, Oakland, CA 94611 USA. [Lanza, Elaine] NCI, Canc Prevent Studies Branch, Rockville, MD 20852 USA. [Schatzkin, Arthur] NCI, Nutr Epidemiol Branch, Rockville, MD 20852 USA. [Brewer, Brenda K.] WESTAT Corp, Rockville, MD 20850 USA. [Slattery, Martha L.] Univ Utah, Sch Med, Div Publ Hlth Sci, Salt Lake City, UT 84108 USA. [Marshall, James R.] Arizona Canc Ctr, Tucson, AZ 85724 USA. RP Caan, BJ (reprint author), Kaiser Permanente, Med Care Program Northern Calif, Div Res, 3505 Broadway, Oakland, CA 94611 USA. EM bjc@dor.kaiser.org NR 19 TC 15 Z9 15 U1 1 U2 1 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD APR PY 1999 VL 2 IS 4 BP 565 EP 569 DI 10.1017/S1368980099000750 PG 5 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA V32XV UT WOS:000208984800012 PM 10656476 ER PT J AU Garraud, O Diouf, A Nguer, CM Dieye, A Longacre, S Kaslow, DC Holder, AA Tall, A Molez, JF Perraut, R Mercereau-Puijalon, O AF Garraud, O Diouf, A Nguer, CM Dieye, A Longacre, S Kaslow, DC Holder, AA Tall, A Molez, JF Perraut, R Mercereau-Puijalon, O TI Different Plasmodium falciparum recombinant MSP1(19) antigens differ in their capacities to stimulate in vitro peripheral blood T lymphocytes in individuals from various endemic areas SO SCANDINAVIAN JOURNAL OF IMMUNOLOGY LA English DT Article ID MEROZOITE SURFACE PROTEIN-1; CARBOXY-TERMINAL FRAGMENT; HUMAN IMMUNE-RESPONSE; DENDRITIC CELLS; B-CELLS; MALARIA MORBIDITY; HOLOENDEMIC AREA; SERUM ANTIBODIES; AOTUS MONKEYS; IN-VITRO AB This study reports on T-cell proliferative responses to the 19-kDa C-terminal domain of the Plasmodium falciparum merozoite surface protein (MSP1(19)). Three different recombinant proteins were used: an Escherichia coli product expressing the first EGF-like domain and Saccharomyces cerevisiae and baculovirus/insect-cell-produced proteins containing both EGF-like domains, the latter protein being produced with or without N-glycosylation. Cell donors were P. falciparum-immune adults with no recent history of clinical malaria and recruited from three Senegalese settings with different epidemiological parasite transmission. Each mononuclear-blood-cell preparation was stimulated with a range of concentrations of the three proteins. Most subjects' mononuclear cells were reactive to at least one protein, but significant differences in lymphoproliferation were seen between the settings and within individual cultures depending on the protein source and concentration. Importantly, lymphoproliferation indices correlated inversely with the intensity of P, falciparum malaria transmission. When purified T lymphocytes were cultured in the presence of MSP1(19) plus autologous monocytes, B lymphocytes or a proposed CD1(+) dendritic-cell population as costimulatory cells, significant differences were observed depending on the individual's previous exposure to parasites. This study shows that the stimulation of lymphocyte proliferation in vitro with MSP1(19) depends on several factors, including epidemiological conditions and protein preparations. C1 Inst Pasteur, Unite Immunol, Dakar, Senegal. Inst Pasteur, Lab Epidemiol Paludisme, Dakar, Senegal. ORSTOM, Dakar, Senegal. NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. Natl Inst Med Res, London, England. RP Garraud, O (reprint author), Inst Pasteur, Unite Immunol, BP 220, Dakar, Senegal. RI Holder, Anthony/A-7554-2013 OI Holder, Anthony/0000-0002-8490-6058 FU Medical Research Council [MC_U117532067] NR 54 TC 11 Z9 11 U1 0 U2 0 PU BLACKWELL SCIENCE LTD PI OXFORD PA P O BOX 88, OSNEY MEAD, OXFORD OX2 0NE, OXON, ENGLAND SN 0300-9475 J9 SCAND J IMMUNOL JI Scand. J. Immunol. PD APR PY 1999 VL 49 IS 4 BP 431 EP 440 DI 10.1046/j.1365-3083.1999.00511.x PG 10 WC Immunology SC Immunology GA 189MV UT WOS:000079909800015 PM 10219771 ER PT J AU Morel, KR Rosenheck, R Henter, ID Wyatt, RJ AF Morel, KR Rosenheck, R Henter, ID Wyatt, RJ TI Stability (interclass reliability) of diagnoses for individuals hospitalized with bipolar disorder, major depressive disorder, and schizophrenia in the Department of Defense who received follow-up care in the Department of Veterans Affairs Medical Centers SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Neuropsychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 9 EP 9 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400016 ER PT J AU Apud, JA Egan, MF Wyatt, RJ AF Apud, JA Egan, MF Wyatt, RJ TI Effects of smoking during antipsychotic withdrawal in patients with chronic schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Ctr Neurosci, Neuropsychiat Branch, Washington, DC 20032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 12 EP 12 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400025 ER PT J AU Bachus, SE Hyde, TM Rubinstein, SL Herman, MM Kleinman, JE AF Bachus, SE Hyde, TM Rubinstein, SL Herman, MM Kleinman, JE TI Hippocampal/caudal entorhinal cortical preprosomatostatin mRNA in schizophrenia and affective disorders SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 69 EP 69 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400188 ER PT J AU Freed, WJ Vawter, MP Freed, LM Webster, MJ AF Freed, WJ Vawter, MP Freed, LM Webster, MJ TI Normal tenascin distribution in the hippocampus in schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIDA, IRP, Baltimore, MD 21224 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 71 EP 71 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400196 ER PT J AU Akil, M Metzger, SS Herman, MM Weickert, CS Hyde, TM Kleinman, JE AF Akil, M Metzger, SS Herman, MM Weickert, CS Hyde, TM Kleinman, JE TI Effects of postnatal development on gene expression of dopamine receptors in the human prefrontal cortex SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 79 EP 79 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400218 ER PT J AU Lenane, H Nicolson, R Bedwell, J Rapoport, JL AF Lenane, H Nicolson, R Bedwell, J Rapoport, JL TI Schizophrenia spectrum disorders in first-degree relatives of patients with childhood-onset schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. RI Nicolson, Robert/E-4797-2011 NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 92 EP 92 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400256 ER PT J AU Nicolson, R Giedd, J Malaspina, D Lenane, M Fernandez, T Bedwell, J Berman, A Susser, E Rapoport, JL AF Nicolson, R Giedd, J Malaspina, D Lenane, M Fernandez, T Bedwell, J Berman, A Susser, E Rapoport, JL TI Obstetrical complications in childhood-onset schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. RI Giedd, Jay/A-3080-2008; Thaker, Gunvant/B-1112-2009; Fernandez, Thomas/D-4295-2009; Nicolson, Robert/E-4797-2011; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015 OI Fernandez, Thomas/0000-0003-0830-022X; Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978 NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 93 EP 93 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400261 ER PT J AU Nicolson, R Hommer, D Thaker, G Brown, M Bedwell, J Lenane, M Fernandez, T Rapoport, JL AF Nicolson, R Hommer, D Thaker, G Brown, M Bedwell, J Lenane, M Fernandez, T Rapoport, JL TI Smooth pursuit eye tracking in the relatives of patients with childhood-onset schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Child Psychiat Branch, Bethesda, MD 20892 USA. RI Giedd, Jay/A-3080-2008; Thaker, Gunvant/B-1112-2009; Fernandez, Thomas/D-4295-2009; Nicolson, Robert/E-4797-2011; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015 OI Fernandez, Thomas/0000-0003-0830-022X; Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978 NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 93 EP 93 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400260 ER PT J AU Elvevag, B Goldberg, TE AF Elvevag, B Goldberg, TE TI Short-term memory for serial order in schizophrenia: A detailed examination of error types SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Clin Brain Disorders Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 165 EP 165 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400470 ER PT J AU Schooler, C AF Schooler, C TI A hypothesized phenomenological micro-gap (75-200 ms) in schizophrenia: Evidence and alternative explanations SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Intramural Res Program, Sect Socioenvironm Studies, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 182 EP 182 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400526 ER PT J AU Holt, JL Van Horn, J Callicott, J Esposito, G Meyer-Lindenberg, A Egan, M Weinberger, DR Berman, KF AF Holt, JL Van Horn, J Callicott, J Esposito, G Meyer-Lindenberg, A Egan, M Weinberger, DR Berman, KF TI Variability of frontal lobe functional neuroanatomy in schizophrenia: Implications for group analyses SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Unit Integrat Neuroimagin, Clin Brain Disorders Branch, IRP,HIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 222 EP 223 PG 2 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400645 ER PT J AU Adler, CM Malhotra, AK Elman, I Carson, R Pickar, D Breier, A AF Adler, CM Malhotra, AK Elman, I Carson, R Pickar, D Breier, A TI Effects of the N-methyl-D-aspariate receptor antagonist ketamine on striatal dopamine release in schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, NIH, Bethesda, MD 20892 USA. RI Carson, Richard/H-3250-2011 OI Carson, Richard/0000-0002-9338-7966 NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 239 EP 239 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400693 ER PT J AU Raedler, TJ Knable, MB Lafargue, T Urbina, RA Egan, MF Pickar, D Weinberger, DR AF Raedler, TJ Knable, MB Lafargue, T Urbina, RA Egan, MF Pickar, D Weinberger, DR TI In vivo determination of muscarinic cholinergic receptor occupancy in patients treated with olanzapine SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Clin Brain Disorders Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 245 EP 245 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400711 ER PT J AU Roberts, BR Schooler, C AF Roberts, BR Schooler, C TI Colour guided saccades: A tool for investigating inhibitory failure in schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Intramural Res Program, Bethesda, MD 20814 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 266 EP 266 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400768 ER PT J AU Pickar, D AF Pickar, D TI Drug withdrawal in schizophrenia does not diminish future drug response SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Expt Therapeut Branch, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 291 EP 292 PG 2 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400845 ER PT J AU Wyatt, RJ Henter, I AF Wyatt, RJ Henter, I TI Long-term effects of discontinuing antipsychotic medication in patients with chronic schizophrenia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Neuropsychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 302 EP 303 PG 2 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400878 ER PT J AU Wyatt, RJ Henter, I Susser, E Mojtabai, R AF Wyatt, RJ Henter, I Susser, E Mojtabai, R TI NCSEPS (National Collaborative Study of Early Psychosis and Suicide): An introduction SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Neuropsychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 302 EP 302 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061400877 ER PT J AU Morel, KR Rosenheck, R Henter, ID Wyatt, RJ AF Morel, KR Rosenheck, R Henter, ID Wyatt, RJ TI Department of veterans affairs health system utilization by patients diagnosed with bipolar disorder, major depressive disorder, or schizophrenia in the department of defense SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Neuropsychiat Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 347 EP 347 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061401001 ER PT J AU Apud, JA Egan, M Weinberger, D Wyatt, RJ Knable, M AF Apud, JA Egan, M Weinberger, D Wyatt, RJ Knable, M TI Effect of gabapentin in the treatment of tardive dyskinesia SO SCHIZOPHRENIA RESEARCH LA English DT Meeting Abstract C1 NIMH, Neuropsychiat Branch, Neurosci Ctr St Elizabeths, Washington, DC 20032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0920-9964 J9 SCHIZOPHR RES JI Schizophr. Res. PD APR PY 1999 VL 36 IS 1-3 SI SI BP 361 EP 361 PG 1 WC Psychiatry SC Psychiatry GA 174XH UT WOS:000079061401037 ER PT J AU Campo, E Raffeld, M Jaffe, ES AF Campo, E Raffeld, M Jaffe, ES TI Mantle-cell lymphoma SO SEMINARS IN HEMATOLOGY LA English DT Review ID NON-HODGKINS-LYMPHOMAS; MAJOR TRANSLOCATION CLUSTER; POLYMERASE CHAIN-REACTION; MARGINAL ZONE LYMPHOMA; IN-SITU HYBRIDIZATION; CYCLIN D1 PROTEIN; T(11-14) CHROMOSOME-TRANSLOCATION; INTERMEDIATE LYMPHOCYTIC LYMPHOMA; HIGH-DOSE CHEMOTHERAPY; BONE-MARROW C1 NCI, Hematopathol Sect, Pathol Lab, NIH, Bethesda, MD 20892 USA. Univ Barcelona, Hematopathol Sect, Pathol Lab, Hosp Clin, Barcelona, Spain. RP Jaffe, ES (reprint author), NCI, Hematopathol Sect, Pathol Lab, NIH, Bldg 10,Room 2N202,10 Ctr Dr,MSC 1500, Bethesda, MD 20892 USA. OI Campo, elias/0000-0001-9850-9793 NR 123 TC 263 Z9 267 U1 0 U2 3 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0037-1963 J9 SEMIN HEMATOL JI Semin. Hematol. PD APR PY 1999 VL 36 IS 2 BP 115 EP 127 PG 13 WC Hematology SC Hematology GA 191YM UT WOS:000080049900003 PM 10319380 ER PT J AU Bartlett, NL Longo, DL AF Bartlett, NL Longo, DL TI T-Small lymphocyte disorders SO SEMINARS IN HEMATOLOGY LA English DT Article ID LARGE GRANULAR LYMPHOCYTES; CELL PROLYMPHOCYTIC LEUKEMIA; POLYMERASE CHAIN-REACTION; PHASE-II TRIAL; MYCOSIS-FUNGOIDES; LENNERTS LYMPHOMA; SEZARY-SYNDROME; LYMPHOPROLIFERATIVE DISORDER; THERAPY; VIRUS C1 Washington Univ, Sch Med, Dept Internal Med, Div Med Oncol, St Louis, MO 63110 USA. NIA, Gerontol Res Ctr, Baltimore, MD 21224 USA. RP Bartlett, NL (reprint author), Washington Univ, Sch Med, Dept Internal Med, Div Med Oncol, 660 S Euclid Ave,Box 8056, St Louis, MO 63110 USA. NR 61 TC 16 Z9 16 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0037-1963 J9 SEMIN HEMATOL JI Semin. Hematol. PD APR PY 1999 VL 36 IS 2 BP 164 EP 170 PG 7 WC Hematology SC Hematology GA 191YM UT WOS:000080049900008 PM 10319385 ER PT J AU Allegra, CJ AF Allegra, CJ TI Antifolates: The next millennium SO SEMINARS IN ONCOLOGY LA English DT Editorial Material C1 NIH, Bethesda, MD 20892 USA. RP Allegra, CJ (reprint author), NIH, Bldg 10,Room 12N226, Bethesda, MD 20892 USA. NR 2 TC 18 Z9 19 U1 0 U2 1 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD APR PY 1999 VL 26 IS 2 SU 6 BP 1 EP 2 PG 2 WC Oncology SC Oncology GA 188WZ UT WOS:000079873400001 PM 10598548 ER PT J AU Shaw, LM Bonner, HS Schuchter, L Schiller, J Lieberman, R AF Shaw, LM Bonner, HS Schuchter, L Schiller, J Lieberman, R TI Pharmacokinetics of amifostine: Effects of dose and method of administration SO SEMINARS IN ONCOLOGY LA English DT Article; Proceedings Paper CT Investigators Workshop on Recent Developments and Emerging Options - The Role of Amifostine as a Broad-Spectrum Cytoprotective Agent CY JAN, 1998 CL PUERTO RICO ID WR-2721; MOUSE; WR-1065; WR2721 C1 Univ Penn, Med Ctr, Ctr Canc, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA. Univ Penn, Med Ctr, Ctr Canc, Dept Hematol & Oncol, Philadelphia, PA 19104 USA. Univ Wisconsin, Ctr Comprehens Canc, Madison, WI USA. NCI, Bethesda, MD 20892 USA. RP Shaw, LM (reprint author), Univ Penn, Dept Pathol, Lab Med, Med Ctr, Philadelphia, PA 19104 USA. NR 9 TC 42 Z9 43 U1 0 U2 0 PU W B SAUNDERS CO PI PHILADELPHIA PA INDEPENDENCE SQUARE WEST CURTIS CENTER, STE 300, PHILADELPHIA, PA 19106-3399 USA SN 0093-7754 J9 SEMIN ONCOL JI Semin. Oncol. PD APR PY 1999 VL 26 IS 2 SU 7 BP 34 EP 36 PG 3 WC Oncology SC Oncology GA 199KY UT WOS:000080481800005 PM 10348258 ER PT J AU Borkowf, CB AF Borkowf, CB TI A new method for approximating the asymptotic variance of Spearman's rank correlation SO STATISTICA SINICA LA English DT Article DE agreement; empirical bivariate quantile-partitioned (EBQP); distribution; epidemiology; nonparametric; quantile correlation; Spearman's rank correlation AB Epidemiologists use Spearman's rank correlation, <(rho)over cap>(s), and the quantile correlation, <(rho)over cap>(q), to measure the agreement between the bivariate ranks and the bivariate quantile-categories of bivariate continuous data, respectively. In this paper ne explore the relationship between the finite and asymptotic means and variances of these statistics. We show that the asymptotic means and variances of <(rho)over cap>(q) converge to the same limits as those of <(rho)over cap>(s), as the number of quantile-categories increases. Also, these point estimates have distributions derived from the "empirical bivariate quantile-partitioned" (EBQP) distribution (Borkowf, Gail, Carroll and Gill (1997)), so we can use nonparametric EBQP methods to estimate the finite variances of these statistics from data and to compute the asymptotic variance of <(rho)over cap>(q) for any underlying bivariate distribution that satisfies certain regularity conditions. These results imply that we can numerically approximate the asymptotic variance of <(rho)over cap>(s), for which an explicit formula is not available except in special cases, to a degree of accuracy limited only by computing power. C1 NHLBI, Div Epidemiol & Clin Applicat, Off Biostat Res, Rockledge Ctr 2, Bethesda, MD 20892 USA. RP Borkowf, CB (reprint author), NHLBI, Div Epidemiol & Clin Applicat, Off Biostat Res, Rockledge Ctr 2, Room 8100D,6701 Rockledge Dr,MSC 7938, Bethesda, MD 20892 USA. NR 19 TC 3 Z9 3 U1 0 U2 1 PU STATISTICA SINICA PI TAIPEI PA C/O DR H C HO, INST STATISTICAL SCIENCE, ACADEMIA SINICA, TAIPEI 115, TAIWAN SN 1017-0405 J9 STAT SINICA JI Stat. Sin. PD APR PY 1999 VL 9 IS 2 BP 535 EP 558 PG 24 WC Statistics & Probability SC Mathematics GA 194NJ UT WOS:000080200200012 ER PT J AU Rosamond, WD Folsom, AR Chambless, LE Wang, CH McGovern, PG Howard, G Copper, LS Shahar, E AF Rosamond, WD Folsom, AR Chambless, LE Wang, CH McGovern, PG Howard, G Copper, LS Shahar, E TI Stroke incidence and survival among middle-aged adults - 9-year follow-up of the Atherosclerosis Risk in Communities (ARIC) cohort SO STROKE LA English DT Article DE cerebral infarction; epidemiology; intracerebral hemorrhage; racial differences ID BLACK-WHITE DIFFERENCES; ETHNIC-DIFFERENCES; UNITED-STATES; CEREBRAL INFARCTION; NORTHERN MANHATTAN; CASE-FATALITY; MORTALITY; POPULATION; DISEASE; TRENDS AB Background and Purpose-Although stroke mortality rates in the United States are well documented, assessment of incidence rates and case fatality are less well studied. Methods-A cohort of 15 792 men and women aged 45 to 64 years from a population sample of households in 4 US communities was followed from 1987 to 1995, an average of 7.2 years. Incident strokes were identified through annual phone contacts and hospital record searching and were then validated. Results-Of the 267 incident definite or probable strokes, 83% (n=221) were categorized as ischemic strokes, 10% (n=27) were intracerebral hemorrhages, and 7% (n=19) were subarachnoid hemorrhages. The age-adjusted incidence rate (per 1000 person-years) of total strokes was highest among black men (4.44), followed by black women (3.10), white men (1.78), and white women(1.24). The black versus white age-adjusted rate ratio (RR) for ischemic stroke was 2.41 (95% CI, 1.85 to 3.15), which was attenuated to 1.38 (95% CI, 1.01 to 1.89) after adjustment for baseline hypertension, diabetes, education level, smoking status, and prevalent coronary heart disease. There was a tendency for the adjusted case fatality rates to be higher among blacks and men, although none of the case fatality comparisons across sex or race was statistically significant. Conclusions-After accounting for established baseline risk:factors, blacks still had a 38% greater risk of incident ischemic stroke compared with whites. Identification of new individual and community-level risk factors accounting for the elevated incidence of stroke requires further investigation and incorporation into intervention planning. C1 Univ N Carolina, Dept Epidemiol, Chapel Hill, NC 27514 USA. Univ N Carolina, Dept Biostat, Collaborat Studies Coordinating Ctr, Chapel Hill, NC 27514 USA. Univ Minnesota, Div Epidemiol, Minneapolis, MN 55455 USA. Wake Forest Univ, Sch Med, Dept Publ Hlth Sci, Winston Salem, NC 27109 USA. NHLBI, Bethesda, MD 20892 USA. RP Rosamond, WD (reprint author), Univ N Carolina, Dept Epidemiol, 137 E Franklin St,Suite 306, Chapel Hill, NC 27514 USA. EM wayne_rosamond@umc.edu FU NHLBI NIH HHS [N01-HC-55016, N01-HC-55015, N01-HC-55018] NR 34 TC 409 Z9 417 U1 1 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0039-2499 J9 STROKE JI Stroke PD APR PY 1999 VL 30 IS 4 BP 736 EP 743 PG 8 WC Clinical Neurology; Peripheral Vascular Disease SC Neurosciences & Neurology; Cardiovascular System & Cardiology GA 180TP UT WOS:000079401500006 PM 10187871 ER PT J AU Xu, H Lu, YF Partilla, JS Zheng, QX Wang, JB Brine, GA Carroll, FI Rice, KC Chen, KX Chi, ZQ Rothman, RB AF Xu, H Lu, YF Partilla, JS Zheng, QX Wang, JB Brine, GA Carroll, FI Rice, KC Chen, KX Chi, ZQ Rothman, RB TI Opioid peptide receptor studies, 11: Involvement of Tyr148, Trp318 and His319 of the rat mu-opioid receptor in binding of mu-selective ligands SO SYNAPSE LA English DT Article DE opioid receptors; ligand binding; fentanyl; molecular modeling; mutagenesis ID OPIATE RECEPTOR; BRAIN AB Previous data obtained with the cloned rat mu opioid receptor demonstrated that the "super-potent" opiates, ohmefentanyl (RTI-4614-4) and its four enantiomers, differ in binding affinity, potency, efficacy, and intrinsic efficacy. Molecular modeling (Tang et al., 1996) of fentanyl derivatives binding to the mu receptor suggests that Asp147, Tyr148, Trp318, and His319 are important residues for binding. According to this model, Asp147 interacts with the positively charged opiate agonist to form potent electrostatic and hydrogen-bonding interactions. In this study, the role of weak electrostatic and hydrogen-bonding "pi-pi" interactions of the O atom of the carbonyl group and the phenyl ring structures of RTI-4614-4 and its four enantiomers with residues Tyr148, Trp318, and His319 were explored via site-directed mutagenesis. Tyr148 tin transmembrane helix 3 {TMH3}), Trp318 (TMH7), and His319 (TMH7) were individually replaced with phenylalanine or alanine. Receptors transiently expressed in COS-7 cells were labeled with [I-125]IOXY according to published procedures. Mutation of Tyr148 to phenylalanine reduced the binding affinities of some mu-selective agonists (2-7 fold) but did not alter the affinities of DAMGO, naloxone, and the non-selective opiates etorphine and buprenorphine. In contrast, this mutation significantly increased the binding affinities (decreased the Kd values) of [D-Al(a)2,D-Leu(5)]enkephalin, IOXY, and dermorphin. Mutation of Trp318 decreased opioid receptor binding to almost undetectable levels. Substitution of alanine for His319 significantly reduced binding affinities for the opioid ligands tested (1.3- to 48-fold), but did not alter the affinities of naloxone and bremazocine. These results indicate the importance of Tyr148 and His319 for the binding of fentanyl derivatives to the mu receptor. Functional studies using the mutant receptors will provide additional insight into the mechanism of action of RTI-4614-4 and its four enantiomers. Synapse 32:23-28, 1999, Published 1999 Wiley-Liss, Inc. C1 NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, Baltimore, MD 21224 USA. UMAB, Sch Pharm, Dept Pharmaceut Sci, Baltimore, MD 21201 USA. Res Triangle Inst, Res Triangle Pk, NC 27709 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China. RP Rothman, RB (reprint author), NIDA, Clin Psychopharmacol Sect, Div Intramural Res, NIH, POB 5180,4940 Eastern Ave, Baltimore, MD 21224 USA. NR 22 TC 37 Z9 38 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0887-4476 J9 SYNAPSE JI Synapse PD APR PY 1999 VL 32 IS 1 BP 23 EP 28 DI 10.1002/(SICI)1098-2396(199904)32:1<23::AID-SYN3>3.0.CO;2-N PG 6 WC Neurosciences SC Neurosciences & Neurology GA 171GW UT WOS:000078855700003 PM 10188634 ER PT J AU Hall, FS Wilkinson, LS Humby, T Robbins, TW AF Hall, FS Wilkinson, LS Humby, T Robbins, TW TI Maternal deprivation of neonatal rats produces enduring changes in dopamine function SO SYNAPSE LA English DT Article DE dopamine; maternal deprivation; microdialysis; d-amphetamine; nucleus accumbens ID SOCIAL-ISOLATION; D-AMPHETAMINE; BENZODIAZEPINE RECEPTOR; PREPULSE INHIBITION; LOCOMOTOR-ACTIVITY; BRAIN; STRESS; SEPARATION; RESPONSES; COCAINE AB Isolation-rearing of weanling rats produces a syndrome of behavioral and neurochemical effects that are indicative of enhanced ventrostriatal dopamine function observed in adulthood. By contrast, maternal deprivation of neonatal rats decreases behavioral responses to dopamine agonists when tested in adults, which may indicate the opposite situation. However, in the present study it is reported that in vivo microdialysis of the nucleus accumbens (NAC) revealed enhanced release of dopamine (DA) in response to both d-amphetamine and high K+ perfusate in maternally deprived subjects. Thus, behavioral responses to d-amphetamine are diminished in maternally deprived rats despite apparent increases in presynaptic dopaminergic function in the NAG. Synapse 32:37-13, 1999, (C) 1999 Wiley-Liss, Inc. C1 Univ Cambridge, Dept Expt Psychol, Cambridge CB2 3EB, England. RP Hall, FS (reprint author), NIDA, Div Intramural Res, POB 5180, Baltimore, MD 21224 USA. EM shall@intra.nida.nih.gov RI Humby, Trevor/A-1698-2010; Hall, Frank/C-3036-2013; OI Hall, Frank/0000-0002-0822-4063; HUMBY, trevor/0000-0002-1840-1799 FU Wellcome Trust NR 35 TC 127 Z9 135 U1 0 U2 3 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0887-4476 J9 SYNAPSE JI Synapse PD APR PY 1999 VL 32 IS 1 BP 37 EP 43 PG 7 WC Neurosciences SC Neurosciences & Neurology GA 171GW UT WOS:000078855700005 PM 10188636 ER PT J AU Villemagne, VL Rothman, RB Yokoi, F Rice, KC Matecka, D Dannals, RF Wong, DF AF Villemagne, VL Rothman, RB Yokoi, F Rice, KC Matecka, D Dannals, RF Wong, DF TI Doses of GBR12909 that suppress cocaine self-administration in non-human primates substantially occupy dopamine transporters as measured by [C-11] WIN35,428 PET scans SO SYNAPSE LA English DT Article DE dopamine transporter; PET; transporter occupancy; non-human primate ID POSITRON EMISSION TOMOGRAPHY; SCHIZOPHRENIC-PATIENTS; ATYPICAL NEUROLEPTICS; RECEPTOR OCCUPANCY; NONHUMAN-PRIMATES; RHESUS-MONKEYS; HUMAN BRAIN; ABUSE; AMPHETAMINE; RAT AB GBR12909 (GBR) is a high-affinity, selective, and long-acting inhibitor of dopamine (DA) uptake that produces a persistent and noncompetitive blockade of DA transporters and substantially reduces cocaine-induced increases in extracellular DA in the nucleus accumbens of rats. Prior studies showed that intravenous infusion of CBR to Rhesus monkeys selectively reduced (1 mg/kg) and eliminated (3 mg/kg) cocaine self-administration, This study tested the hypothesis that doses of GBR, that reduce cocaine self-administration in nonhuman primates produce significant occupation of DA transporters. DA transporters were quantitated in two baboons using [C-11]WIN35,428 and positron emission tomography (PET). Each baboon underwent paired control/ blocked PET scans (performed on three separate study days, 3-4 weeks apart). On the first scan the baboon received saline (3 ml/kg) 90 minutes before the injection of the radiotracer. GBR (1 mg/kg i.v.) was infused 90 minutes before the second [C-11]WIN 35,428 study. The same experimental design was repeated with GBR doses of 3 and 10 mg/kg, respectively. Doses of 1 (n = 2), 3 mg/kg (n = 2), and 10 mg/kg (n = 2) reduced binding potential by 26, 53, and 72%, respectively. GBR was well tolerated in all baboons. These results demonstrate that doses of GBR that suppress cocaine self-administration in nonhuman primates also produce high occupancy of the DA transporter. These data strongly suggest that occupancy for the DA transporter by GBR explains its ability to attenuate cocaine-induced increases in extracellular DA and to suppress cocaine self-administration. Moreover, these data suggest that experimental human studies of orally administered GBR to test the DA hypothesis of cocaine addiction should use doses that produce at least 70% occupancy of the DA transporter. Synapse 32:44-50, 1999. (C) 1999 Wiley-Liss, Inc. C1 Johns Hopkins Med Inst, JHOC, Dept Radiol, Baltimore, MD 21287 USA. NIDDK, LMC, NIH, Bethesda, MD 20892 USA. NIDA, CPS, IRP, NIH, Baltimore, MD 21224 USA. RP Wong, DF (reprint author), Johns Hopkins Med Inst, JHOC, Dept Radiol, Room 3245,601 N Caroline St, Baltimore, MD 21287 USA. FU NIDA NIH HHS [1 RO1DA09482] NR 67 TC 44 Z9 45 U1 1 U2 4 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0887-4476 J9 SYNAPSE JI Synapse PD APR PY 1999 VL 32 IS 1 BP 44 EP 50 DI 10.1002/(SICI)1098-2396(199904)32:1<44::AID-SYN6>3.0.CO;2-9 PG 7 WC Neurosciences SC Neurosciences & Neurology GA 171GW UT WOS:000078855700006 PM 10188637 ER PT J AU Boice, JD Miller, RW AF Boice, JD Miller, RW TI Childhood and adult cancer after intrauterine exposure to ionizing radiation SO TERATOLOGY LA English DT Article; Proceedings Paper CT Annual Meeting of the National Council for Radiation Protection (NCRP) CY APR 02-03, 1997 CL CRYSTAL CITY, VIRGINIA SP Natl Council Radiat Protect ID ATOMIC-BOMB SURVIVORS; X-RAY-EXPOSURE; IN-UTERO; PRENATAL IRRADIATION; CHERNOBYL ACCIDENT; INFANT LEUKEMIA; SWEDISH TWINS; RISK; CHILDREN; MORTALITY AB Since the reports in 1956 and 1958 that in utero radiation was associated with an increased risk of leukemia and solid cancers during childhood, this issue has been debated. Many epidemiological studies have been performed. Evidence for a causal association derives almost entirely from case-control studies, whereas practically all cohort studies find no association, most notably the series of atomic bomb survivors exposed in utero. Although it is likely that in utero radiation presents a leukemogenic risk to the fetus, the magnitude of the risk remains uncertain. The causal nature of the risk of cancers other than leukemia is less convincing, and the similar relative risks (RR = 1.5) for virtually all forms of childhood cancer suggests an underlying bias. Few studies have addressed the potential risk of adult cancer after intrauterine exposure. Radiotherapy given to newborns, however, has been linked to cancers of the thyroid and breast later in life. (C) 1999 Wiley-Liss, Inc. C1 Int Epidemiol Inst, Rockville, MD 20850 USA. NCI, Bethesda, MD 20892 USA. RP Boice, JD (reprint author), Int Epidemiol Inst, 1500 Res Blvd,Suite 210, Rockville, MD 20850 USA. NR 57 TC 79 Z9 86 U1 0 U2 3 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0040-3709 J9 TERATOLOGY JI Teratology PD APR PY 1999 VL 59 IS 4 BP 227 EP 233 DI 10.1002/(SICI)1096-9926(199904)59:4<227::AID-TERA7>3.0.CO;2-E PG 7 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA 190UF UT WOS:000079983100007 PM 10331524 ER PT J AU Miller, RW AF Miller, RW TI Severe mental retardation and cancer among atomic bomb survivors exposed in utero - Discussion SO TERATOLOGY LA English DT Editorial Material ID MORTALITY AB When I was in medical school, Douglas Power Murphy, Professor of Obstetrics and Gynecology, told us of his inexpensive, simple study of "microcephaly" and mental retardation in newborn infants whose mothers had received therapeutic radiation early in pregnancy. His review of the literature and mail inquiry of other obstetrics centers in the United States revealed 14 published cases (Murphy, '28) and 16 unpublished (Goldstein and Murphy, '29). Here am I, 52 years later, still updating his findings. (C) 1999 Wiley-Liss, Inc. C1 NCI, Bethesda, MD 20892 USA. RP Miller, RW (reprint author), NCI, EPS 7018, Bethesda, MD 20892 USA. NR 10 TC 29 Z9 30 U1 1 U2 4 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0040-3709 J9 TERATOLOGY JI Teratology PD APR PY 1999 VL 59 IS 4 BP 234 EP 235 DI 10.1002/(SICI)1096-9926(199904)59:4<234::AID-TERA8>3.0.CO;2-B PG 2 WC Developmental Biology; Toxicology SC Developmental Biology; Toxicology GA 190UF UT WOS:000079983100008 PM 10331525 ER PT J AU Suzuki, K Mori, A Lavaroni, S Miyagi, E Ulianich, L Katoh, R Kawaoi, A Kohn, LD AF Suzuki, K Mori, A Lavaroni, S Miyagi, E Ulianich, L Katoh, R Kawaoi, A Kohn, LD TI In vivo expression of thyroid transcription factor-1 RNA and its relation to thyroid function and follicular heterogeneity: Identification of follicular thyroglobulin as a feedback suppressor of thyroid transcription factor-1 RNA levels and thyroglobulin synthesis SO THYROID LA English DT Article ID THYROTROPIN RECEPTOR GENE; CELL NUCLEAR ANTIGEN; GROWTH FACTOR-I; TRIIODOTHYRONINE T3; STIMULATING HORMONE; THYROXINE T4; RAT; PEROXIDASE; PROMOTER; PROTEIN AB We used in situ hybridization to evaluate thyroid transcription factor-1 (TTF-1) RNA expression in individual follicles and related this to thyroglobulin (Tg) synthesis in vivo, as estimated by immunohistochemical analysis. We studied the thyroids of Wistar rats treated with thyroxine (T-4) Or propylthiouracil (PTU), each of which modulates TSH levels, but affects follicular function and Tg accumulation in the follicular lumen very differently. We show that TTF-1 RNA levels in vivo correlate directly with an increase in the cytoplasmic accumulation of Tg within the cells of individual follicles. Because TTF-I increases Tg gene expression, RNA levels, and protein synthesis in thyroid cell cultures and because there is no correlation with TSH-increased Tg degradation within the follicular lumen, the increased cytoplasmic accumulation of Tg in vivo is interpreted to reflect TTF-1-increased Tg synthesis. Increases in serum TSH levels in the PTU or T-4 treated animals did not always correlate with increases in this measure of increased Tg synthesis; and TSH levels did not always correlate with changes in TTF-1 RNA levels that would be expected to accompany increased Tg synthesis. As one possibility, this suggested there might be a hitherto unrecognized suppressor of TTF-1 RNA levels and TSH-induced Tg synthesis in individual follicles. The immunohistochemical data suggested that this suppressor might be follicular Tg itself. Supporting this possibility, we show that physiological concentrations of highly purified 19S follicular Tg decrease TTF-1 RNA levels in rat FRTL-5 thyroid cells and inhibit the action of TSH to increase Tg synthesis. We therefore suggest that follicular Tg is a feedback autoregulator of thyroid function that can counterregulate TSH actions on thyroid function in vivo and in thyroid cells in culture. We suggest this phenomenon contributes to follicular heterogeneity in vivo. C1 NIDDK, Cell Regulat Sect, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. Yamanashi Med Univ, Dept Pathol, Yamanashi, Japan. RP Kohn, LD (reprint author), NIDDK, Cell Regulat Sect, Metab Dis Branch, NIH, Bldg 10,Room 9C101B, Bethesda, MD 20892 USA. NR 43 TC 37 Z9 38 U1 0 U2 2 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 1050-7256 J9 THYROID JI Thyroid PD APR PY 1999 VL 9 IS 4 BP 319 EP 331 DI 10.1089/thy.1999.9.319 PG 13 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 190BU UT WOS:000079942300001 PM 10319936 ER PT J AU Chung, HK Kim, WB Park, DJ Kohn, LD Tahara, K Cho, BY AF Chung, HK Kim, WB Park, DJ Kohn, LD Tahara, K Cho, BY TI Two Graves' disease patients who spontaneously developed hypothyroidism after antithyroid drug treatment: Characteristics of epitopes for thyrotropin receptor antibodies SO THYROID LA English DT Article ID THYROID-STIMULATING ANTIBODIES; TSH RECEPTOR; IDIOPATHIC MYXEDEMA; HYPERTHYROIDISM; IMMUNOGLOBULINS; REMISSION; THERAPY; SERA AB Few reports have identified blocking thyrotropin receptor antibodies (TSHRAbs) as a pathogenic mechanism explaining spontaneous hypothyroidism after antithyroid drug (ATD) treatment of Graves' disease. Here we report 2 Graves' patients who showed different courses of hypothyroidism after ATD treatment. The first patient had Graves' hyperthyroidism and was treated with ATD for 1 year. After a short period of euthyroidism, she developed permanent hypothyroidism with blocking TSHRAb. The second patient became euthyroid after 1 year of ATD treatment. After 3 years, however, she presented with hypothyroidism with blocking TSHRAb activity. Her hypothyroidism was transient, and restoration of euthyroidism was followed by disappearance of blocking TSHRAb. Blocking and stimulating TSHRAbs activities of these 2 patients were serially measured using Chinese hamster ovary (CHO) cells transfected with wild-type human TSHR (CHO-hTSHR) and 2 TSHR chimeras with residues 8-165 (Mc1+2) or 90-165 (Mc2) substituted by equivalent residues of the luteinizing hormone/chorionic gonadotropin receptor (LH/CGR). During their hypothyroid phases, blocking TSHRAbs activities were positive in all 3 kinds of assays and stimulating TSHRAbs activities were negative in CHO-hTSHR or in Mc1+2 assay. Mc2 stimulating TSHRAb activity was detected in sera of hypothyroid phase of the second patient who had transient hypothyroidism but not in the first whose hypothyroidism was permanent. In these 2 cases, we demonstrate the causative role of blocking TSHRAb in the development of hypothyroidism after ATD treatment in Graves' patients. Interestingly, the difference in the course of blocking TSHRAb-induced hypothyroidism was associated with the difference in epitope reactivities of TRAb during hypothyroid phase that developed after ATD treatment of Graves' disease. C1 Seoul Natl Univ, Coll Med, Dept Internal Med, Seoul 151, South Korea. NIDDK, Cell Regulat Sect, Metab Dis Branch, NIH, Bethesda, MD USA. Chiba Univ, Sch Med, Dept Internal Med 2, Div Endocrinol & Metab, Chiba 280, Japan. RP Cho, BY (reprint author), Seoul Natl Univ Hosp, Dept Internal Med, 28 Yongun Dong, Seoul 110744, South Korea. RI Park, Do-Joon/J-2736-2012 NR 18 TC 8 Z9 8 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 1050-7256 J9 THYROID JI Thyroid PD APR PY 1999 VL 9 IS 4 BP 393 EP 399 DI 10.1089/thy.1999.9.393 PG 7 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 190BU UT WOS:000079942300012 PM 10319947 ER PT J AU Bhat, MK Dace, A Cheng, SY AF Bhat, MK Dace, A Cheng, SY TI Tissue-specific differential repression of gene expression by a dominant negative mutant of thyroid hormone beta 1 receptor SO THYROID LA English DT Article; Proceedings Paper CT 70th Annual Meeting of the American-Thyroid-Association CY OCT 14-19, 1997 CL COLORADO SPRINGS, COLORADO SP Amer Thyroid Assoc ID TUMOR-SUPPRESSOR P53; TRANSCRIPTIONAL ACTIVITY; NUCLEAR RECEPTORS; T3 RECEPTOR; RESISTANCE; ACTIVATION; ANTIBODIES; BINDING; PIT-1; CELLS AB Resistance to thyroid hormone (RTH) is a genetic disease caused by the mutations of the thyroid hormone beta receptor (TR beta) gene, producing receptors with a dominant negative action. The present study addressed the question as to whether tissue-specific factors modulate the dominant negative function in different tissues. We prepared stably transfected pituitary GH3 (GH3-PV) and liver SK-Hep-1 (SK-Hep-1-PV) cell lines with a potent dominant negative mutant, PV. The growth hormone (GH) and the malic enzyme genes (ME) in GH3 and SK-Hep-l, respectively, are directly regulated by the thyroid hormone, 3,3,'5-triiodo-L-thyronine (T-3) The ratio of the expressed PV/endogenous TR beta(1) proteins was approximately 20 and 5 for GH3-PV and SK-Hep-1-PV cells, respectively. However, the T-3-activated expression of the GH gene in GH3-PV and ME gene in SK-Hep-1-PV was repressed by approximately 30% and 90%, respectively, indicating the lack of correlation of PV/TR beta(1) protein ratio with the dominant negative potency of mutant PV. Furthermore, the synergistic effect of the pituitary-specific factor 1 on the TR-mediated CH promoter activity was not repressed by mutant PV. Taken together, these results suggest that the dominant negative effect of mutant TR is variable in the tissues studied. C1 NCI, Mol Biol Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. RP Cheng, SY (reprint author), NCI, Mol Biol Lab, Div Basic Sci, NIH, Bldg 37,Rm 2D-24,37 Convent Dr,MSC 4255, Bethesda, MD 20892 USA. NR 21 TC 5 Z9 5 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 1050-7256 J9 THYROID JI Thyroid PD APR PY 1999 VL 9 IS 4 BP 411 EP 418 DI 10.1089/thy.1999.9.411 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 190BU UT WOS:000079942300015 PM 10319950 ER PT J AU Cohen, MD Schook, LB Oppenheim, JJ Freed, BM Rodgers, KE AF Cohen, MD Schook, LB Oppenheim, JJ Freed, BM Rodgers, KE TI Symposium overview: Alterations in cytokine receptors by xenobiotics SO TOXICOLOGICAL SCIENCES LA English DT Article ID INTERFERON RECEPTORS; DOWN-REGULATION; FACTOR-ALPHA; IFN-GAMMA; HYDROQUINONE; EXPRESSION; CELLS; INHIBITION; PROLIFERATION; MACROPHAGES AB A symposium entitled Alterations in Cytokine Receptors by Xenobiotics was held at the 137th Annual Meeting of the Society of Toxicology (SOT) in Seattle, Washington. The symposium was sponsored by the Immunotoxicology Specialty Section of SOT and was designed to present information on the effect of several different classes of xenobiotics on various aspects of receptor function (i.e., post-receptor signal transduction of receptor expression), or the involvement of cytokine receptors in the action of the toxicant under consideration. This symposium brought together scientists in the area of receptor immunobiology whose expertise in receptor modulation encompassed those major signaling agents involved in the normal immune response, i.e., proinflammatory, cytokines, chemokines, interleukins, and interferons. The following is a summary of each of the individual presentations. C1 NYU, Med Ctr, Dept Environment Med, Tuxedo, NY 10987 USA. Univ Minnesota, Coll Vet Med, Ctr Food Animal Bioltechnol, St Paul, MN 55108 USA. Natl Canc Inst, Lab Mol Immunoregulat, Frederick, MD 21701 USA. Univ Colorado, Dept Med, Ctr Hlth Sci, Boulder, CO 80262 USA. Univ So Calif, Livingston Res Inst, Los Angeles, CA 90033 USA. RP Cohen, MD (reprint author), NYU, Med Ctr, Dept Environment Med, 57 Old Forge Rd, Tuxedo, NY 10987 USA. OI Schook, Lawrence/0000-0002-6580-8364 NR 23 TC 7 Z9 7 U1 0 U2 0 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1096-6080 J9 TOXICOL SCI JI Toxicol. Sci. PD APR PY 1999 VL 48 IS 2 BP 163 EP 169 DI 10.1093/toxsci/48.2.163 PG 7 WC Toxicology SC Toxicology GA 251PW UT WOS:000083454900014 PM 10353307 ER PT J AU Newlin, DB AF Newlin, DB TI Evolutionary game theory and multiple chemical sensitivity SO TOXICOLOGY AND INDUSTRIAL HEALTH LA English DT Article ID SELF-HANDICAPPING STRATEGY; ALCOHOL-CONSUMPTION; WHITE PAPER; SENSITIZATION; ASYMMETRY; TOLERANCE; ADDICTION; COCAINE; HEART AB Newlin's [Newlin D.B. Evolutionary game theory of tolerance and sensitization in substance abuse. Paper presented to the Research Society on Alcoholism, Hilton Head, SC, 1998] evolutionary game theory of addictive behavior specifies how evolutionarily stable strategies for survival and reproduction may lead to addiction. The game theory of multiple chemical sensitivity (MCS) assumes that: (1) the MCS patient responds to low-level toxicants as stressors eras direct threats to their survival and reproductive fitness, (2) this activates the cortico-mesolimbic dopamine system, (3) this system is a survival motivation center-not a 'reward center', (4) the subject emits a counter-response that is in the same direction as the naive response to the chemicals, (5) previously neutral stimuli associated with chemicals also trigger conditioned responses that mimic those to the chemicals. (6) these counter-responses further activate the dopaminergic survival motivation system, and (7) this produces a positive feedback loop that leads to strong neural sensitization in these structures and in behavior controlled by this system, despite a small initial response. Psychologically, the MCS patient with a sensitized cortico-mesolimbic dopamine system is behaving as though his/her survival is directly threatened by these chemicals. Non-MCS subjects have counter-responses opposite in direction to those of the chemicals and show tolerance. An autoshaping/sign-tracking model of this game is discussed. This evolutionary game makes several specific, testable predictions about differences between MCS subjects, non-MCS controls, and substance abusers in laboratory experiments, and between sensitized and nonsensitized animals. C1 NIDA, Intramural Res Program, Baltimore, MD 21224 USA. RP Newlin, DB (reprint author), NIDA, Intramural Res Program, 5500 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 56 TC 8 Z9 8 U1 2 U2 3 PU STOCKTON PRESS PI NEW YORK PA 345 PARK AVE SOUTH, NEW YORK, NY 10010-1707 USA SN 0748-2337 J9 TOXICOL IND HEALTH JI Toxicol. Ind. Health PD APR-JUN PY 1999 VL 15 IS 3-4 BP 313 EP 322 PG 10 WC Public, Environmental & Occupational Health; Toxicology SC Public, Environmental & Occupational Health; Toxicology GA 295BK UT WOS:000085945900005 PM 10416283 ER PT J AU Stroncek, DF Hubel, A Shankar, RA Burger, SR Pan, D McCullough, J Whitley, CB AF Stroncek, DF Hubel, A Shankar, RA Burger, SR Pan, D McCullough, J Whitley, CB TI Retroviral transduction and expansion of peripheral blood lymphocytes for the treatment of mucopolysaccharidosis type II, Hunter's syndrome SO TRANSFUSION LA English DT Article ID MEDIATED GENE-TRANSFER; TUMOR-INFILTRATING LYMPHOCYTES; KILLER-CELLS; THERAPY; IDURONATE-2-SULFATASE; GENERATION; VEHICLES AB BACKGROUND: Gene therapy using autologous peripheral blood lymphocytes (PBLs) has been used to produce adenosine deaminase with which to treat patients with severe combined immunodeficiency. Patients with mucopolysaccharidosis type II (MPS II) lack iduronate-2-sulfatase (IDS), and serial PBL gene therapy may benefit these patients. STUDY DESIGN AND METHODS: The purpose of these studies was to develop a method to transduce PBLs from a patient with MPS If by using a retroviral vector, LS2N, containing the IDS gene. PBLs were collected by apheresis and cryopreserved in aliquots for the performance of multiple transductions and expansions. The PBLs were expanded in number and then transduced in a hollow-fiber bioreactor (HFBR). Additional culture allowed for further expansion. RESULTS: Fresh PBLs (6.2 x 10(7)) from a patient with MPS II were transduced with L2SN and expanded in an HFBR with an extracapillary space of 11 mL. After 10 days of culture, 4.1 x 10(9) cells were harvested. Cryopreserved MPS It PBLs could not be reliably expanded in they were placed in the HFBR immediately after being thawed; however, cells were successfully transduced and expanded in the HFBR if they were first cultured in a bag. To increase the cell yield, PBLs were expanded in a 60-mL HFBR after transduction and expansion in an 11-mL HFBR. In four separate experiments, 2 x 10(8) cryopreserved PBL were cultured for 3 days in a bag and transferred to an 11-mL HFBR, where they were transduced daily with L2SN for 3 days and then expanded for 4 additional days. Cells were then transferred into a 60-mL HFBR and expanded for an additional 7 days. In the four experiments, 5.5 x 10(9), 7.4 x 10(9), 1.12 x 10(9), and 19.4 x 10(9) cells were produced. The vector was detected in the harvested cells, but the proportion of cells transduced was less than 2.5 percent, the lowest standard used in the assay. In two of the experiments, cells harvested from the HFBR were used in a gene therapy clinical trial. CONCLUSION: Autologous cryopreserved PBLs can be transduced and expanded to produce >1 x 10(10) cells. This procedure is being used for a Phase I/II clinical trial of lymphocyte gene therapy. C1 Univ Minnesota, Dept Lab Med & Pathol, Cell Therapy Clin Lab, Minneapolis, MN 55455 USA. Univ Minnesota, Sch Med, Dept Pediat, Minneapolis, MN 55455 USA. Univ Minnesota, Inst Human Genet, Gene Therapy Program, Minneapolis, MN 55455 USA. RP Stroncek, DF (reprint author), NIH, Dept Transfus Med, Warren G Magnuson Clin Ctr, 10 Ctr Dr,MSC-1184,Bldg 10,Room 1C711, Bethesda, MD 20892 USA. NR 18 TC 19 Z9 21 U1 0 U2 2 PU AMER ASSOC BLOOD BANKS PI BETHESDA PA 8101 GLENBROOK RD, BETHESDA, MD 20814-2749 USA SN 0041-1132 J9 TRANSFUSION JI Transfusion PD APR PY 1999 VL 39 IS 4 BP 343 EP 350 DI 10.1046/j.1537-2995.1999.39499235664.x PG 8 WC Hematology SC Hematology GA 186ZA UT WOS:000079760400002 PM 10220258 ER PT J AU Ponting, CP Aravind, L AF Ponting, CP Aravind, L TI START: a lipid binding domain in StAR, HD-ZIP and signalling proteins SO TRENDS IN BIOCHEMICAL SCIENCES LA English DT Article ID LIPOID ADRENAL-HYPERPLASIA; GENE; IDENTIFICATION; ARABIDOPSIS C1 Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. RP Ponting, CP (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, NIH, Bethesda, MD 20894 USA. NR 18 TC 269 Z9 279 U1 2 U2 5 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0968-0004 J9 TRENDS BIOCHEM SCI JI Trends Biochem.Sci. PD APR PY 1999 VL 24 IS 4 BP 130 EP 132 DI 10.1016/S0968-0004(99)01362-6 PG 3 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 199ZR UT WOS:000080514400004 PM 10322415 ER PT J AU Murray, EA Bussey, TJ AF Murray, EA Bussey, TJ TI Perceptual-mnemonic functions of the perirhinal cortex SO TRENDS IN COGNITIVE SCIENCES LA English DT Review ID MEDIAL TEMPORAL-LOBE; LONG-TERM-MEMORY; RECOGNITION MEMORY; RHINAL CORTEX; INFEROTEMPORAL CORTEX; PARAHIPPOCAMPAL CORTICES; OBJECT-RECOGNITION; HIPPOCAMPAL-FORMATION; ENTORHINAL CORTEX; RHESUS-MONKEYS AB It is widely acknowledged that the perirhinal cortex, located in the ventromedial aspect of the temporal lobe, is essential for certain types of memory in macaque monkeys. For example, removal of the perirhinal cortex yields severe impairments on tests of stimulus recognition and stimulus-stimulus association. There is considerable disagreement, however, about the most accurate way to characterize the function of the perirhinal cortex; some views emphasize a role in perception whereas others posit a role exclusively in declarative memory. In this article, we review recent findings from anatomical, physiological and ablation studies in monkeys, and discuss related findings obtained in humans, in an attempt to identify not only the cognitive functions of the perirhinal cortex, but also the implications of these findings for theoretical views concerning the organization of memory. C1 NIMH, Neuropsychol Lab, Bethesda, MD 20892 USA. RP Murray, EA (reprint author), NIMH, Neuropsychol Lab, Bldg 49,Room IB80, Bethesda, MD 20892 USA. EM eam@ln.nimh.nih.gov; bussey@ln.nimh.nih.gov RI Bussey, Timothy/M-2758-2016; OI Bussey, Timothy/0000-0001-7518-4041; Murray, Elisabeth/0000-0003-1450-1642 NR 88 TC 311 Z9 312 U1 2 U2 6 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1364-6613 J9 TRENDS COGN SCI JI TRENDS COGN. SCI. PD APR PY 1999 VL 3 IS 4 BP 142 EP 151 DI 10.1016/S1364-6613(99)01303-0 PG 10 WC Behavioral Sciences; Neurosciences; Psychology, Experimental SC Behavioral Sciences; Neurosciences & Neurology; Psychology GA 181AM UT WOS:000079418500005 ER PT J AU Weinstein, LS Yu, SH AF Weinstein, LS Yu, SH TI The role of genomic imprinting of G(s)alpha in the pathogenesis of Albright hereditary osteodystrophy SO TRENDS IN ENDOCRINOLOGY AND METABOLISM LA English DT Review ID GS-ALPHA GENE; CYCLASE COUPLING PROTEIN; NUCLEOTIDE REGULATORY PROTEIN; GRADIENT GEL-ELECTROPHORESIS; STIMULATORY G-PROTEIN; HUMAN GNAS1 GENE; DEFICIENT ACTIVITY; BINDING PROTEIN; HORMONE RESISTANCE; PARENTAL ORIGIN AB Albright hereditary osteodystrophy (AHO) is caused by heterozygous inactivating mutations of the gene encoding the alpha-subunit of the G protein G(s). The G(s)alpha gene is a complex gene that uses various alternative promoters and produces various protein products. Recently it has been shown that this gene is imprinted in a tissue-specific manner The role of tissue-specific imprinting of G(s)alpha in the pathogenesis of AHO is discussed. C1 NIDDK, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. RP Weinstein, LS (reprint author), NIDDK, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. OI Weinstein, Lee/0000-0002-1899-5152 NR 40 TC 27 Z9 28 U1 0 U2 1 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1043-2760 J9 TRENDS ENDOCRIN MET JI Trends Endocrinol. Metab. PD APR PY 1999 VL 10 IS 3 BP 81 EP 85 DI 10.1016/S1043-2760(98)00124-6 PG 5 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 214LR UT WOS:000081329900001 ER PT J AU Chou, JY Mansfield, BC AF Chou, JY Mansfield, BC TI Molecular genetics of type 1 glycogen storage diseases SO TRENDS IN ENDOCRINOLOGY AND METABOLISM LA English DT Review ID MICROSOMAL GLUCOSE-6-PHOSPHATASE SYSTEM; ENZYME-DEFICIENT; CATALYTIC SUBUNIT; CHINESE PATIENTS; POINT MUTATION; MESSENGER-RNA; IB; IDENTIFICATION; EXPRESSION; TRANSPORT AB Glycogen storage disease type 1 (GSD-1), also known as von Gierke disease, is caused by a deficiency in the activity of the enzyme glucose-6-phosphatase (G6Pase). It is an autosomal recessive disorder characterized by hypoglycemia, hepatomegaly, kidney enlargement growth retardation, lactic acidemia, hyperlipidemia and hyperuricemia. The disease presents with both clinical and biochemical heterogeneity consistent with the existence of two major subgroups, GSD-1a and GSD-1b, which have been confirmed at the molecular genetic level. GSD-1a, the most prevalent form, is caused by mutations in the G6Pase gene that abolish or greatly reduce enzymatic activity. The gene maps to chromosome 17q21 and encodes a microsomal transmembrane protein. Animal models of GSD-1a exist and are being exploited to delineate the disease more precisely. It has been proposed that GSD-1b is caused by a defect in the microsomal glucose-6-phosphate transporter. The gene responsible for GSD-1b has been mapped to chromosome 11q23 and a cDNA encoding a microsomal transmembrane protein has been identified. The function of this putative GSD-1b protein remains to be determined. These recent developments, along with newly characterized animal models of GSD-1a, ave increasing our understanding of the interrelationship between the components of the G6Pase complex and type 1 glycogen storage diseases. C1 NICHHD, Heritable Disorders Branch, NIH, Bethesda, MD 20892 USA. RP Chou, JY (reprint author), NICHHD, Heritable Disorders Branch, NIH, Bethesda, MD 20892 USA. NR 48 TC 45 Z9 45 U1 2 U2 7 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1043-2760 J9 TRENDS ENDOCRIN MET JI Trends Endocrinol. Metab. PD APR PY 1999 VL 10 IS 3 BP 104 EP 113 PG 10 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 214LR UT WOS:000081329900005 ER PT J AU Koonin, E AF Koonin, E TI Why genome analysis? SO TRENDS IN GENETICS LA English DT Editorial Material C1 NIH, Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. RP Koonin, E (reprint author), NIH, Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0168-9525 J9 TRENDS GENET JI Trends Genet. PD APR PY 1999 VL 15 IS 4 BP 131 EP 131 DI 10.1016/S0168-9525(99)01721-7 PG 1 WC Genetics & Heredity SC Genetics & Heredity GA 181BC UT WOS:000079419900004 PM 10203815 ER PT J AU Heinzen, RA Hackstadt, T Samuel, JE AF Heinzen, RA Hackstadt, T Samuel, JE TI Developmental biology of Coxiella burnetii SO TRENDS IN MICROBIOLOGY LA English DT Review ID CHLAMYDIA-TRACHOMATIS; ESCHERICHIA-COLI; Q-FEVER; HOST-CELLS; HISTONE H1; PROTEIN; GROWTH; PHASE; ANTIBODIES; MICROSCOPY AB The obligate intracellular bacterial agent of human Q fever, Coxiella burnetii, has a remarkable ability to persist in the extracellular environment. It replicates only when phagocytosed and delivered to the phagolysosome, where it resists degradation. Different morphological forms of the bacterium have different resistance properties and appear to be stages of a developmental cycle. Despite the lack of genetic systems, the molecular events surrounding C. burnetii development are now being unraveled. C1 Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA. NIAID, Rocky Mt Labs, Intracellular Parasites Lab, Host Paraite Interact Sect, Hamilton, MT 59840 USA. Texas A&M Univ, Hlth Sci Ctr, Dept Med Microbiol & Immunol, College Stn, TX 77843 USA. RP Heinzen, RA (reprint author), Univ Wyoming, Dept Mol Biol, Laramie, WY 82071 USA. FU NIAID NIH HHS [AI37744] NR 32 TC 116 Z9 121 U1 2 U2 10 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0966-842X J9 TRENDS MICROBIOL JI Trends Microbiol. PD APR PY 1999 VL 7 IS 4 BP 149 EP 154 DI 10.1016/S0966-842X(99)01475-4 PG 6 WC Biochemistry & Molecular Biology; Microbiology SC Biochemistry & Molecular Biology; Microbiology GA 215JW UT WOS:000081379400006 PM 10217829 ER PT J AU Nemes, Z Steinert, PM AF Nemes, Z Steinert, PM TI Bricks and mortar of the epidermal barrier SO EXPERIMENTAL AND MOLECULAR MEDICINE LA English DT Review ID CORNIFIED CELL-ENVELOPE; PROLINE-RICH PROTEINS; HUMAN INVOLUCRIN GENE; KERATIN INTERMEDIATE FILAMENTS; RECESSIVE LAMELLAR ICHTHYOSIS; CROSS-LINKED ENVELOPE; TRANSGLUTAMINASE SUBSTRATE PROPERTIES; CULTURED HUMAN KERATINOCYTES; ELASTASE-SPECIFIC INHIBITOR; SJOGREN-LARSSON-SYNDROME AB A specialized tissue type, the keratinizing epithelium, protects terrestrial mammals from water loss and noxious physical, chemical and mechanical insults. This barrier between the body and the environment is constantly maintained by reproduction of inner living epidermal keratinocytes which undergo a process of terminal differentiation and then migrate to the surface as interlocking layers of dead stratum corneum cells. These cells provide the bulwark of mechanical and chemical protection, and together with their intercellular lipid surroundings, confer water-impermeability. Much of this barrier function is provided by the cornified cell envelope (CE), an extremely tough protein/lipid polymer structure formed just below the cytoplasmic membrane and subsequently resides on the exterior of the dead cornified cells. It consists of two parts: a protein envelope and a lipid envelope, The protein envelope is thought to contribute to the biomechanical properties of the CE as a result of cross-linking of specialized CE structural proteins by both disulfide bonds and N-epsilon-(gamma-glutamyl)lysine isopeptide bonds formed by transglutaminases, Some of the structural proteins involved include involucrin, loricrin, small proline rich proteins, keratin intermediate filaments, elafin, cystatin A, and desmosomal proteins, The lipid envelope is located on the exterior of and covalently attached by ester bonds to the protein envelope and consists of a monomolecular layer of omega-hydroxyceramides, These not only serve of provide a Teflon-like coating to the cell, but also interdigitate with the intercellular lipid lamellae perhaps in a Velcro-like fashion. In fact the CE is a common feature of all stratified squamous epithelia, although its precise composition, structure and barrier function requirements vary widely between epithelia, Recent work has shown that a number of diseases which display defective epidermal barrier function, generically known as ichthyoses, are the result of genetic defects of the synthesis of either CE proteins, the transglutaminase 1 cross-linking enzyme, or defective metabolism of skin lipids. C1 NIAMSD, Skin Biol Lab, NIH, Bethesda, MD 20892 USA. EM pemast@helix.nih.gov NR 206 TC 285 Z9 290 U1 4 U2 32 PU KOREAN SOC MED BIOCHEMISTRY MOLECULAR BIOLOGY PI SEOUL PA #812 KOFST, 635-4 YOKSAM-DONG KANGNAM-GU, SEOUL 135-703, SOUTH KOREA SN 1226-3613 J9 EXP MOL MED JI Exp. Mol. Med. PD MAR 31 PY 1999 VL 31 IS 1 BP 5 EP 19 PG 15 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA 184BG UT WOS:000079589000002 PM 10231017 ER PT J AU Kreitman, RJ Wang, QQ FitzGerald, DJP Pastan, I AF Kreitman, RJ Wang, QQ FitzGerald, DJP Pastan, I TI Complete regression of human B-cell lymphoma xenografts in mice treated with recombinant anti-CD22 immunotoxin RFB4(dsFv)-PE38 at doses tolerated by cynomolgus monkeys SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID RICIN-A-CHAIN; STABILIZED FV-IMMUNOTOXINS; PSEUDOMONAS EXOTOXIN-A; VASCULAR LEAK SYNDROME; HUMAN ENDOTHELIAL-CELLS; PHASE-I; SINGLE-CHAIN; NUDE-MICE; CONTINUOUS-INFUSION; HUMAN CARCINOMA AB RFB4(dsFv)-PE38 is a recombinant immunotoxin in which the variable light domain (V-L) is disulfide bonded via cysteine residues to the variable heavy domain (V-H), which in turn is fused to PE38, a mutant form of Pseudomonas exotoxin A. RFB4 binds: to CD22, which is a differentiation antigen expressed an the majority of B-cell leukemias and lymphomas, To examine the potential efficacy of RFB4(dsFv)-PE38 when administered at a dose schedule appropriate for phase I testing, mice bearing CA46 human CD22(+) Burkitt's lymphoma xenografts were treated on alternate days i.v. for 3 doses (QOD x3), Complete regressions were observed in 80% and 100% of mice treated with 200 and 275 mu g/kg QOD x3, respectively, The higher dose was 27% of the LDS, and 34% of the LD10 in mice. Because RFB4(dsFv)-PE38 is stable at 37 degrees C, it could also be given by continuous infusion using pumps placed in the peritoneal cavity; complete regressions also resulted from this mode of administration, To study toxicology, a pilot: toxicology study of RFB4(dsFv)-PE38 was undertaken in cynomolgus monkeys, which like humans but: unlike mice have CD22, which binds RFB4, Doses of 100 and 500 mu g/kg i.v. QOD x3 were well tolerated, indicating that a dose that cared tumors in mice was tolerated by primates. Based on these preclinical results, RFB4(dsFv)-PE38 is being developed for the treatment of patients with CD22-positive leukemias and lymphomas. Published 1999 Wiley-Liss, Inc. C1 NCI, Mol Biol Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. RP Pastan, I (reprint author), NCI, Mol Biol Lab, Div Basic Sci, NIH, Bldg 37,Room 4E16,37 Convent Dr MSC 4255, Bethesda, MD 20892 USA. NR 34 TC 72 Z9 78 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 31 PY 1999 VL 81 IS 1 BP 148 EP 155 PG 8 WC Oncology SC Oncology GA 173CE UT WOS:000078963000024 PM 10077166 ER PT J AU Cornilescu, G Hu, JS Bax, A AF Cornilescu, G Hu, JS Bax, A TI Identification of the hydrogen bonding network in a protein by scalar couplings SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID CHEMICAL-SHIFT ANISOTROPY; QUANTITATIVE MEASUREMENT; RELAXATION; SPECTROSCOPY; LENGTH C1 NIDDKD, Chem Phys Lab, NIH, Bethesda, MD 20892 USA. RP Bax, A (reprint author), NIDDKD, Chem Phys Lab, NIH, Bldg 2, Bethesda, MD 20892 USA. RI Cornilescu, Gabriel/H-3113-2011 OI Cornilescu, Gabriel/0000-0002-1204-8904 NR 22 TC 198 Z9 201 U1 1 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD MAR 31 PY 1999 VL 121 IS 12 BP 2949 EP 2950 DI 10.1021/ja9902221 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 182QR UT WOS:000079510100047 ER PT J AU Marsolais, F Laviolette, M Kakuta, Y Negishi, M Pedersen, LC Auger, M Varin, L AF Marsolais, F Laviolette, M Kakuta, Y Negishi, M Pedersen, LC Auger, M Varin, L TI 3 '-phosphoadenosine 5 '-phosphosulfate binding site of flavonol 3-sulfotransferase studied by affinity chromatography and P-31 NMR SO BIOCHEMISTRY LA English DT Article ID AMINO-ACID-SEQUENCE; ESTROGEN SULFOTRANSFERASE; STEROID SULFOTRANSFERASES; PHENOL SULFOTRANSFERASE; MUTATIONAL ANALYSIS; KINETIC-PROPERTIES; CRYSTAL-STRUCTURE; ADENYLATE KINASE; PURIFICATION; MECHANISM AB The function of Lys-59, Arg-141, and Arg-277 in PAPS binding and catalysis of the flavonol 3-sulfotransferase was investigated. Affinity chromatography of conservative mutants with PAPS analogues allowed us to determine that Lys-59 interacts with the 5' portion of the nucleotide, while Arg-141 interacts with the 3' portion, confirming assignments deduced from the crystal structure of mouse estrogen sulfotransferase [Kakuta, Y., Pedersen, L. G., Carter, C. W., Negishi, M., and Pedersen, L. C. (1997) Nat. Struct. Biol. 4, 904-908]. The affinity chromatography method could be used to characterize site-directed mutants for other types of enzymes that bind nucleoside 3',5'- or 2',5'-diphosphates. P-31 NMR spectra of enzyme-PAP complexes were recorded for the wild-type enzyme and K59R and K59A mutants. The results of these experiments suggest that Lys-59 is involved in the determination of the proper orientation of the phosphosulfate group for catalysis. C1 Concordia Univ, Dept Biol, Montreal, PQ H3G 1M8, Canada. Univ Laval, Ctr Rech Sci & Ingn Macromol, Dept Chim, St Foy, PQ G1K 7P4, Canada. NIEHS, Reprod & Dev Toxicol Lab, Pharmacogenet Sect, NIH, Res Triangle Pk, NC 27709 USA. RP Varin, L (reprint author), Concordia Univ, Dept Biol, 1455 De Maisonneuve Blvd W, Montreal, PQ H3G 1M8, Canada. EM varinl@clone.concordia.ca NR 29 TC 12 Z9 13 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 30 PY 1999 VL 38 IS 13 BP 4066 EP 4071 DI 10.1021/bi982239m PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 182QZ UT WOS:000079510800026 PM 10194320 ER PT J AU Mori, Y Shiota, T Jones, M Wanitkun, S Irvine, T Li, XK Delabays, A Pandian, NG Sahn, DJ AF Mori, Y Shiota, T Jones, M Wanitkun, S Irvine, T Li, XK Delabays, A Pandian, NG Sahn, DJ TI Three-dimensional reconstruction of the color Doppler-imaged vena contracta for quantifying aortic regurgitation - Studies in a chronic animal model SO CIRCULATION LA English DT Article DE blood flow; regurgitation; echocardiography; imaging ID FLOW MAPPING PREDICTS; MITRAL REGURGITATION; ORIFICE AREA; IN-VITRO; BLOOD-FLOW; SEVERITY; JET; VALVE; INVITRO; SHEEP AB Background-The purpose of this study was to investigate the use of 3-dimensional (3D) reconstruction of color Doppler flow maps to image and extract the vena contracta cross-sectional area to determine the severity of aortic regurgitation (AR) in an animal model. Evaluation of the vena contracts with 2-dimensional imaging systems may not be sufficiently robust to fully characterize this region, which may be asymmetrically shaped. Methods and Results-In 6 sheep with surgically induced chronic AR, 18 hemodynamically different states were studied. instantaneous regurgitant flow rates were obtained by aortic and pulmonary electromagnetic flowmeters (EMFs) as reference standards, and aortic regurgitant effective orifice areas (EOAs) were determined from EMF regurgitant flow rates divided by continuous-wave (CW) Doppler velocities, Composite video data for color Doppler imaging of the aortic regurgitant flows were transferred into a TomTec computer after computer-controlled 180 degrees rotational acquisition. After the 3D data transverse to the flow jet were sectioned, the smallest proximal jet cross section was identified for direct measurement of the vena contracta area. Peak regurgitant flow rates and regurgitant stroke volumes were calculated as the product of these areas and the CW Doppler peak velocities and velocity-time integrals, respectively. There was an excellent correlation between the 3D-derived vena contracta areas and reference EOAs (r=0.99, SEE=0.01 cm(2)) and between 3D and reference peak regurgitant flow rates and regurgitant stroke volumes (r=0.99, difference=0.11 L/min; r=0.99, difference=1.5 mL/beat, respectively). Conclusions-3D-based determination of the vena contracta cross-sectional area can provide accurate quantification of the severity of AR. C1 NHLBI, NIH, Lab Anim Med & Surg, Bethesda, MD 20892 USA. Oregon Hlth Sci Univ, Clin Care Ctr Congenital Heart Dis, Portland, OR 97201 USA. Cleveland Clin Fdn, Cleveland, OH 44195 USA. Tuffs New England Med Ctr, Noninvas Cardiac Lab, Boston, MA USA. RP Jones, M (reprint author), NHLBI, NIH, Lab Anim Med & Surg, 9000 Rockville Pike,Bldg 14E,Room 1074A, Bethesda, MD 20892 USA. NR 35 TC 36 Z9 40 U1 0 U2 2 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD MAR 30 PY 1999 VL 99 IS 12 BP 1611 EP 1617 PG 7 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 179TJ UT WOS:000079344900014 PM 10096939 ER PT J AU Sondej, M Sun, JZ Seok, YJ Kaback, HR Peterkofsky, A AF Sondej, M Sun, JZ Seok, YJ Kaback, HR Peterkofsky, A TI Deduction of consensus binding sequences on proteins that bind IIA(Glc) of the phosphoenolpyruvate : sugar phosphotransferase system by cysteine scanning mutagenesis of Escherichia coli lactose permease SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID POLYTOPIC MEMBRANE-PROTEINS; LAC CARRIER PROTEIN; SALMONELLA-TYPHIMURIUM; ALLOSTERIC REGULATION; MALTOSE TRANSPORT; INDUCER EXCLUSION; ENZYME-III; INHIBITION; RESISTANT; RESIDUES AB Mediated by the protein IIA(Glc), the phosphocnolpyruvate:sugar phosphotransferase system plays a role in the regulation of activity of other sugar transport systems in Escherichia coli, By using a direct binding assay, a collection of single-Cys replacement mutants in cytoplasmic loops of lactose permease were evaluated for their capacity to bind IIa(Glc). Selected Cys replacements in loops IV/V or VI/VII result in loss of binding activity. Analysis of the mutagenesis results together with multiple sequence alignments of a family of proteins that interacts with IIA(Glc) provides the basis for developing two regions of consensus sequence in those partner proteins necessary for binding to IIA(Glc). The requirement for two interaction regions is interpreted in the regulatory framework of a substrate-dependent conformational change that brings those two regions into an orientation optimal for binding IIA(Glc).(.) C1 NHLBI, Biochem Genet Lab, NIH, Bethesda, MD 20892 USA. Seoul Natl Univ, Coll Nat Sci, Dept Microbiol, Seoul 151742, South Korea. Univ Calif Los Angeles, Inst Mol Biol, Howard Hughes Med Inst, Dept Physiol, Los Angeles, CA 90024 USA. Univ Calif Los Angeles, Inst Mol Biol, Howard Hughes Med Inst, Dept Microbiol & Mol Genet, Los Angeles, CA 90024 USA. RP Peterkofsky, A (reprint author), NHLBI, Biochem Genet Lab, NIH, Bldg 36,Room 4C-11, Bethesda, MD 20892 USA. EM alan@codon.nih.gov NR 28 TC 18 Z9 19 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3525 EP 3530 DI 10.1073/pnas.96.7.3525 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900034 PM 10097069 ER PT J AU Curtis, SW Clark, J Myers, P Korach, KS AF Curtis, SW Clark, J Myers, P Korach, KS TI Disruption of estrogen signaling does not prevent progesterone action in the estrogen receptor or knockout mouse uterus SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID CALCITONIN-GENE EXPRESSION; DIFFERENTIAL REGULATION; GROWTH-FACTORS; ACID; MICE; IMPLANTATION; TISSUE; CELLS; RNA AB Estrogen is known to increase progesterone receptor (PR) levels in the wild-type mouse uterus, and this estrogen induction was thought to he important for progesterone action through the PR. The estrogen receptor alpha knockout (ERKO) mouse uterus was observed to express PR mRNA that cannot be induced by estrogen, Progesterone action mas characterized to determine whether it was diminished in ERKO mice. The PR protein is present in the ERKO uterus at 60% of the level measured in a wild-type uterus, The PR-A and PR-B isoforms are both detected on Western blot, and the ratio of isoforms is the same in both genotypes, Although the level of PR is reduced in the ERKO uterus, the receptor level is sufficient to induce genomic responses, since both calcitonin and amphiregulin mRNAs were increased after progesterone treatment. Finally, the ERKO uterus can be induced to undergo a progesterone-dependent decidual response, Surprisingly, the decidual response is estrogen independent in the ERKO, although it remains estrogen dependent in a wild type, These results indicate that estrogen receptor alpha modulation of PR levels is not necessary for expression of the PR or genomic and physiologic responses to progesterone in the ERKO uterus. C1 NIEHS, Receptor Biol Sect, Res Triangle Pk, NC 27709 USA. NIEHS, Comparat Med Branch, Res Triangle Pk, NC 27709 USA. RP Korach, KS (reprint author), NIEHS, Receptor Biol Sect, POB 12233, Res Triangle Pk, NC 27709 USA. EM korach@nichs.nih.gov NR 30 TC 85 Z9 86 U1 0 U2 3 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3646 EP 3651 DI 10.1073/pnas.96.7.3646 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900056 PM 10097091 ER PT J AU Wang, XW Zhan, QM Coursen, JD Khan, MA Kontny, HU Yu, LJ Hollander, MC O'Connor, PM Fornace, AJ Harris, CC AF Wang, XW Zhan, QM Coursen, JD Khan, MA Kontny, HU Yu, LJ Hollander, MC O'Connor, PM Fornace, AJ Harris, CC TI GADD45 induction of a G(2)/M cell cycle checkpoint SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID WILD-TYPE P53; ATAXIA-TELANGIECTASIA GENE; DNA-DAMAGE; IONIZING-RADIATION; TUMOR-SUPPRESSOR; GROWTH ARREST; PROTEIN; APOPTOSIS; REPAIR; KINASE AB G(1)/S and G(2)/M cell cycle checkpoints main tain genomic stability in eukaryotes in response to genotoxic stress. We report here both genetic and Functional evidence of a Gadd45-mediated G(2)/M checkpoint in human and murine cells. Increased expression of Gadd45 via microinjection of an expression vector into primary human fibroblasts arrests the cells at the G(2)/M boundary with a phenotype of MPM2 immunopositivity, 4n DNA content and, in 15% of the cells, centrosome separation. The Gadd45-mediated G(2)/M arrest depends on wild-type p53, because no arrest was observed either in p53-null Li-Fraumeni fibroblasts or in normal fibroblasts coexpressed with p53 mutants. Increased expression of cyclin B1 and Cdc25C inhibited the Gadd45-mediated G(2)/M arrest in human fibroblasts, indicating that the mechanism of Gadd45-mediated G(2)/M checkpoint is at least in part through modulation of the activity of the G(2)-specific kinase, cyclin B1/p34(cdc2). Genetic and physiological evidence of a Gadd45-mediated G(2)/M checkpoint was obtained by using GADD45-deficient human or murine cells. Human cells with endogenous Gadd45 expression reduced by antisense GADD45 expression have an impaired G(2)/M checkpoint after exposure to either ultraviolet radiation or methyl methanesulfonate but are still able to undergo G(2) arrest after ionizing radiation. Lymphocytes from gadd45-knockout mice (gadd45 -/-) also retained a G(2)/M checkpoint initiated by ionizing radiation and failed to arrest at G(2)/M after exposure to ultraviolet radiation. Therefore, the mammalian genome is protected by a multiplicity of G(2)/M checkpoints in response to specific types of DNA damage. C1 NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. NCI, Biol Chem Lab, NIH, Bethesda, MD 20892 USA. NCI, Mol Pharmacol Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. RP Harris, CC (reprint author), NCI, Human Carcinogenesis Lab, NIH, Bldg 37,Room 2C05, Bethesda, MD 20892 USA. EM curtis_harris@nih.gov RI Wang, Xin/B-6162-2009; Fornace, Albert/A-7407-2008 OI Fornace, Albert/0000-0001-9695-085X NR 65 TC 439 Z9 454 U1 2 U2 14 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3706 EP 3711 DI 10.1073/pnas.96.7.3706 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900066 PM 10097101 ER PT J AU Brown, AL Lee, CH Schwarz, JK Mitiku, N Piwnica-Worms, H Chung, JH AF Brown, AL Lee, CH Schwarz, JK Mitiku, N Piwnica-Worms, H Chung, JH TI A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PHOSPHORYLATES HUMAN CDC25C; ATAXIA-TELANGIECTASIA GENE; SACCHAROMYCES-CEREVISIAE; FISSION YEAST; SCHIZOSACCHAROMYCES-POMBE; S-PHASE; MEIOTIC RECOMBINATION; TRANSCRIPTION FACTORS; IONIZING-RADIATION; CHECKPOINT PATHWAY AB Checkpoints maintain the order and fidelity of the eukaryotic cell cycle, and defects in checkpoints contribute to genetic instability and cancer. Much of our current understanding of checkpoints comes from genetic studies conducted in yeast. In the fission yeast Schizosaccharomyces pombe (Sp), SpRad3 is an essential component of both the DNA damage and DNA replication checkpoints. The SpChk1 and SpCds1 protein kinases function downstream of SpRad3. SpChk1 is an effector of the DNA damage checkpoint and, in the absence of SpCds1, serves an essential function in the DNA replication checkpoint. SpCds1 functions in the DNA replication checkpoint and in the S phase DNA damage checkpoint, Human homologs of both SpRad3 and SpChk1 but not SpCds1 have been identified. Here we report the identification of a human cDNA encoding a protein (designated HuCds1) that shares sequence, structural, and functional similarity to SpCds1, HuCds1 was modified by phosphorylation and activated in response to ionizing radiation. It was also modified in response to hydroxyurea treatment. Functional ATM protein was required for HuCds1 modification after ionizing radiation but not after hydroxyurea treatment. Like its fission yeast counterpart, human Cds1 phosphorylated Cdc25C to promote the binding of 14-3-3 proteins. These findings suggest that the checkpoint function of HuCds1 is conserved in yeast and mammals. C1 NHLBI, Mol Hematol Branch, NIH, Bethesda, MD 20892 USA. Washington Univ, Sch Med, Dept Cell Biol & Physiol, St Louis, MO 63110 USA. Washington Univ, Sch Med, Howard Hughes Med Inst, St Louis, MO 63110 USA. RP Chung, JH (reprint author), NHLBI, Mol Hematol Branch, NIH, Bldg 10-7D13,10 Ctr Dr, Bethesda, MD 20892 USA. EM jhchung@helix.nih.gov RI Piwnica-Worms, Helen/C-5214-2012 NR 61 TC 225 Z9 227 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3745 EP 3750 DI 10.1073/pnas.96.7.3745 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900073 PM 10097108 ER PT J AU Srivastava, RK Mi, QS Hardwick, JM Longo, DL AF Srivastava, RK Mi, QS Hardwick, JM Longo, DL TI Deletion of the loop region of Bcl-2 completely blocks paclitaxel-induced apoptosis SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PROGRAMMED CELL-DEATH; PROTEIN-KINASE; CYTOCHROME-C; SIGNAL-TRANSDUCTION; PHOSPHORYLATION; ACTIVATION; PATHOGENESIS; REQUIREMENT; SUPPRESSION; SERINE-70 AB At high concentrations, the tubule poison paclitaxel is able to kill cancer cells that express Bcl-2; it inhibits the antiapoptotic activity of Bcl-2 by inducing its phosphorylation. To localize the site on Bcl-2 regulated by phosphorylation, mutant forms of Bcl-2 were constructed. Mutant forms of Bcl-2 with an alteration in serine at amino acid 70 (S70A) or,vith deletion of a 60-aa loop region between the alpha 1 and alpha 2 helices (Delta loop Bcl-2, which also deletes amino acid 70) were unable to be phosphorylated by paclitaxel treatment of MDA-MB-231 cells into which the genes for the mutant proteins were transfected, The Delta loop mutant completely inhibited paclitaxel-induced apoptosis, In cells expressing the S70A mutant, paclitaxel induced about one-third the level of apoptosis seen with wild-type Bcl-2, To evaluate the role of mitogen-activated protein kinases (MAPKs) in Bcl-2 phosphorylation, the activation of c-jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK, and p38 was examined. Paclitaxel induced apoptosis was associated with phosphorylation of Bcl-2 and activation of ERK and JNK MAPKs. If JNK activation was blocked by transfections with either a stress-activated protein kinase kinase dominant-negative (K-->R) gene (which prevents the activation of a kinase upstream of JNK) or MAPK phosphatase-l gene (which dephosphorylates and inactivates JNK), Bcl-2 phosphorylation did not occur, and the cells were not killed by paclitaxel. By contrast, neither an ERK inhibitor (PD098059) nor p38 inhibitors (SB203580 and SB202190) had an effect on Bcl-2 phosphorylation, Thus, our data show that the antiapoptotic effects of Bcl-2 can be overcome by phosphorylation of Ser-70; forms of Bcl-2 lacking the loop region are much more effective at preventing apoptosis than wild-type Bcl-2 because they cannot be phosphorylated, JNK, but not ERK or p38 MAPK, appear to be involved in the phosphorylation of Bcl-2 induced by paclitaxel. C1 NIA, Immunol Lab, NIH, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Publ Hlth, Dept Mol Biol & Immunol, Baltimore, MD 21205 USA. RP Srivastava, RK (reprint author), NIA, Immunol Lab, NIH, 5600 Nathan Shock Dr,Box 28, Baltimore, MD 21224 USA. NR 42 TC 286 Z9 290 U1 1 U2 6 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3775 EP 3780 DI 10.1073/pnas.96.7.3775 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900078 PM 10097113 ER PT J AU Snyder, GA Brooks, AG Sun, PD AF Snyder, GA Brooks, AG Sun, PD TI Crystal structure of the HLA-Cw3 allotype-specific killer cell inhibitory receptor KIR2DL2 SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID CLASS-I MOLECULES; HUMAN TISSUE FACTOR; HLA-C; DIRECT BINDING; IMMUNOGLOBULIN-SUPERFAMILY; ANTIGEN RECEPTOR; EXTRACELLULAR DOMAIN; FUNCTIONAL TRANSFER; GROWTH-HORMONE; FACTOR VIIA AB Killer cell inhibitory receptors (KIR) protect class I HLAs expressing target cells from natural killer (NK) cell-mediated lysis, To understand the molecular basis of this receptor-ligand recognition, we have crystallized the extracellular ligand-binding domains of KIR2DL2, a member of the Ig superfamily receptors that recognize HLA-Cw1, 3, 7, and 8 allotypes, The structure was determined in two different crystal forms, an orthorhombic P2(1)2(1)2(1) and a trigonal P3(2)21 space group, to resolutions of 3.0 and 2.9 Angstrom, respectively. The overall fold of this structure, like KIR2DL1, exhibits K-type Ig topology with cis-proline residues in both domains that define beta-strand switching, which sets KIR apart from the C2-type hematopoietic growth hormone receptor fold, The hinge angle of KIR2DL2 is approximately 80 degrees, 14 degrees larger than that observed in KIR2DL1 despite the existence of conserved hydrophobic residues near the hinge region. There is also a 5 degrees difference in the observed hinge angles in two crystal forms of 2DL2. suggesting that the interdomain hinge angle is not fixed. The putative ligand-binding site is formed by residues from several variable loops with charge distribution apparently complementary to that of HLA-C, The packing of the receptors in the orthorhombic crystal form offers an intriguing model for receptor aggregation on the cell surface. C1 NIAID, Struct Biol Sect, Off Sci Director, NIH, Rockville, MD 20852 USA. Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA. Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia. RP Sun, PD (reprint author), NIAID, Struct Biol Sect, Off Sci Director, NIH, 12441 Parklawn Dr, Rockville, MD 20852 USA. EM sun@magenta.niaid.nih.gov NR 57 TC 93 Z9 94 U1 1 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3864 EP 3869 DI 10.1073/pnas.96.7.3864 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900094 PM 10097129 ER PT J AU Zhang, ZJ Kundu, GC Panda, D Mandal, AK Mantile-Selvaggi, G Peri, A Yuan, CJ Mukherjee, AB AF Zhang, ZJ Kundu, GC Panda, D Mandal, AK Mantile-Selvaggi, G Peri, A Yuan, CJ Mukherjee, AB TI Loss of transformed phenotype in cancer cells by overexpression of the uteroglobin gene SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID RABBIT UTEROGLOBIN; PLATELET-AGGREGATION; CHROMOSOME 11Q13; 10-KDA PROTEIN; BREAST-CANCER; AMINO-ACID; EXPRESSION; BINDING; PEPTIDES; MICE AB Uteroglobin (UG) is a multifunctional, secreted protein that has receptor-mediated functions. The human UG (hUG) gene is mapped to chromosome 11q12.2-13.1, a region frequently rearranged or deleted in many cancers. Although high levels of hUG expression are characteristic of the mucosal epithelia of many organs, hUG expression is either drastically reduced or totally absent in adenocarcinomas and in viral-transformed epithelial cells derived from the same organs. In agreement with these findings, in an ongoing study to evaluate the effects of aging on UG-knockout mice, 16/16 animals developed malignant tumors, whereas the wild-type littermates (n = 25) remained apparently healthy even after 1 1/2 years. In the present investigation, we sought to determine the effects of induced-expression of hUG in human cancer cells by transfecting several cell lines derived from adenocarcinomas of various organs with an hUG-cDNA construct. We demonstrate that induced hUG expression reverses at least two of the most important characteristics of the transformed phenotype (i.e., anchorage-independent growth on soft agar and extracellular matrix invasion) of only those cancer cells that also express the hUG receptor. Similarly, treatment of the nontransfected, receptor-positive adenocarcinoma cells with purified recombinant hUG yielded identical results. Taken together, these data define receptor-mediated, autocrine and paracrine pathways through which hUG reverses the transformed phenotype of cancer cells and consequently, may have tumor suppressor-like effects. C1 NICHHD, Sect Dev Genet, Heritable Disorders Branch, NIH, Bethesda, MD 20892 USA. RP Mukherjee, AB (reprint author), NICHHD, Sect Dev Genet, Heritable Disorders Branch, NIH, Bldg 10,Room 9S241, Bethesda, MD 20892 USA. EM mukherja@exehange.nih.gov NR 36 TC 43 Z9 44 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 3963 EP 3968 DI 10.1073/pnas.96.7.3963 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900111 PM 10097146 ER PT J AU Bons, N Mestre-Frances, N Belli, P Cathala, F Gajdusek, DC Brown, P AF Bons, N Mestre-Frances, N Belli, P Cathala, F Gajdusek, DC Brown, P TI Natural and experimental oral infection of nonhuman primates by bovine spongiform encephalopathy agents SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID MICROCEBUS-MURINUS; TAU-PROTEINS; SCRAPIE VIRUS; PATHOGENESIS; BRAIN AB Experimental lemurs either were infected orally with the agent of bovine spongiform encephalopathy (BSE) or were maintained as uninfected control animals. Immunohistochemical examination for proteinase-resistant protein (prion protein or PrP) was performed on tissues from two infected but still asymptomatic lemurs, killed 5 months after infection, and from three uninfected control lemurs, Control tissues shelved no staining, whereas PrP was detected in the infected animals in tonsil, gastrointestinal tract and associated lymphatic tissues, and spleen. In addition, PrP was detected in ventral and dorsal roots of the cervical spinal cord, and within the spinal cord PrP could be traced in nerve tracts as far as the cerebral cortex. Similar patterns of PrP immunoreactivity were seen in two symptomatic and 18 apparently healthy lemurs in three different French primate centers, all of which had been fed diets supplemented with a beef protein product manufactured by a British company that has since ceased to include beef in its veterinary nutritional products. This study of BSE-infected lemurs early in their incubation period extends previous pathogenesis studies of the distribution of infectivity and PrP in natural and experimental scrapie, The similarity of neuropathology and PrP immunostaining patterns in experimentally infected animals to those observed in both symptomatic and asymptomatic animals in primate centers suggests that BSE contamination of zoo animals may have been more widespread than is generally appreciated. C1 Univ Montpellier 2, Ecole Prat Hautes Etud, Lab Neuromorphol Fonct, F-34095 Montpellier 5, France. Ctr Natl Etud Vet & Alimentaires, F-69342 Lyon 07, France. CNRS, Inst Alfred Fessard, F-91198 Gif Sur Yvette, France. NINDS, Cent Nervous Syst Studies Lab, NIH, Bethesda, MD 20892 USA. RP Bons, N (reprint author), Univ Montpellier 2, Ecole Prat Hautes Etud, Lab Neuromorphol Fonct, F-34095 Montpellier 5, France. EM ephemeb@crit.univ-montp2.fr NR 15 TC 137 Z9 141 U1 4 U2 10 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 4046 EP 4051 DI 10.1073/pnas.96.7.4046 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900125 PM 10097160 ER PT J AU Van Goor, F Krsmanovic, LZ Catt, KJ Stojilkovic, SS AF Van Goor, F Krsmanovic, LZ Catt, KJ Stojilkovic, SS TI Coordinate regulation of gonadotropin-releasing hormone neuronal firing patterns by cytosolic calcium and store depletion SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PITUITARY CELL-LINE; K+ CHANNELS; POTASSIUM CHANNELS; INOSITOL PHOSPHATE; SMALL-CONDUCTANCE; GNRH NEURONS; PATCH-CLAMP; APAMIN; CA2+; OSCILLATIONS AB Elevation of cytosolic free Ca2+ concentration ([Ca2+](i)) in excitable cells often acts as a negative feedback signal on firing of action potentials and the associated voltage-gated Ca2+ influx. Increased [Ca2+](i) stimulates Ca2+-sensitive K+ channels (IK-Ca), and this, in turn, hyperpolarizes the cell and inhibits Ca2+ influx. However, in some cells expressing IK-Ca the elevation in [Ca2+](i) by depletion of intracellular stores facilitates voltage-gated Ca2+ influx. This phenomenon was studied in hypothalamic GT1 neuronal cells during store depletion caused by activation of gonadotropin-releasing hormone (GnRH) receptors and inhibition of endoplasmic reticulum (Ca2+)ATPase with thapsigargin, GnRH induced a rapid spike increase in [Ca2+](i) accompanied by transient hyperpolarization, followed by a sustained [Ca2+](i) plateau during which the depolarized cells fired with higher frequency. The transient hyperpolarization was caused by the initial spike in [Ca2+](i) and was mediated by apamin-sensitive IK-Ca channels, which also were operative during the subsequent depolarization phase. Agonist-induced depolarization and increased firing were independent of [Ca2+](i) and were not mediated by inhibition of K+ current, but by facilitation of a voltage-insensitive, Ca2+-conducting inward current. Store depletion by thapsigargin also activated this inward depolarizing current and increased the firing frequency. Thus, the pattern of firing in GT1 neurons is regulated coordinately by apamin-sensitive SK current and store depletion-activated Ca2+ current. This dual control of pacemaker activity facilitates voltage-gated Ca2+ influx at elevated [Ca2+](i) levels, but also protects cells from Ca2+ overload. This process may also provide a general mechanism for the integration of voltage-gated Ca2+ influx into receptor-controlled Ca2+ mobilization. C1 NICHHD, SCS, Endocrinol & Reprod Res Branch, NIH, Bethesda, MD 20892 USA. RP Stojilkovic, SS (reprint author), NICHHD, SCS, Endocrinol & Reprod Res Branch, NIH, Bldg 49,Room 6A-36,49 Convent Dt, Bethesda, MD 20892 USA. EM stankos@helix.nih.gov NR 30 TC 52 Z9 52 U1 0 U2 0 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 4101 EP 4106 DI 10.1073/pnas.96.7.4101 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900135 PM 10097170 ER PT J AU Kittles, RA Long, JC Bergen, AW Eggert, M Virkkunen, M Linnoila, M Goldman, D AF Kittles, RA Long, JC Bergen, AW Eggert, M Virkkunen, M Linnoila, M Goldman, D TI Cladistic association analysis of Y chromosome effects on alcohol dependence and related personality traits SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID CROSS-FOSTERING ANALYSIS; ALPHOID SATELLITE DNA; PHENOTYPIC ASSOCIATIONS; ANTISOCIAL PERSONALITY; LINKAGE DISEQUILIBRIUM; ENVIRONMENTAL-FACTORS; INTERMALE AGGRESSION; FINNISH POPULATION; HEALTHY-VOLUNTEERS; VIOLENT OFFENDERS AB Association between Y chromosome haplotype variation and alcohol dependence and related personality traits was investigated in a large sample of psychiatrically diagnosed Finnish males. Haplotypes were constructed for 359 individuals using alleles at eight loci (seven microsatellite loci and a nucleotide substitution in the DYZ3 alphoid satellite locus). A cladogram linking the 102 observed haplotype configurations was constructed by using parsimony with a single-step mutation model. Then, a series of contingency tables nested according to the cladogram hierarchy were used to test for association between Y haplotype and alcohol dependence. Finally, using only alcohol-dependent subjects, we tested for association between Y haplotype and personality variables postulated to define subtypes of alcoholism-antisocial personality disorder, novelty seeking, harm avoidance, and reward dependence. Significant association with alcohol dependence was observed at three Y haplotype clades, with significance levels of P = 0.002, P = 0.020, and P = 0.010, Within alcohol-dependent subjects, no relationship was revealed between Y haplotype and antisocial personality disorder, novelty seeking, harm avoidance, or reward dependence. These results demonstrate, by using a fully objective association design, that differences among Y chromosomes contribute to variation in vulnerability to alcohol dependence. However, they do not demonstrate an association between Y haplotype and the personality variables thought to underlie the subtypes of alcoholism. C1 NIAAA, Sect Populat Genet & Linkage, NIH, Bethesda, MD 20892 USA. NIAAA, Neurogenet Lab, NIH, Bethesda, MD 20892 USA. NIAAA, Clin Studies Lab, NIH, Bethesda, MD 20892 USA. Univ Helsinki, Dept Psychiat, SF-00180 Helsinki, Finland. RP Long, JC (reprint author), NIAAA, Sect Populat Genet & Linkage, NIH, 12420 Parklawn Dr,Pk 5 Bldg,Room 451, Bethesda, MD 20892 USA. EM jcl@box-j.nih.gov RI Goldman, David/F-9772-2010; OI Goldman, David/0000-0002-1724-5405; Bergen, Andrew/0000-0002-1237-7644 NR 52 TC 28 Z9 28 U1 2 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 30 PY 1999 VL 96 IS 7 BP 4204 EP 4209 DI 10.1073/pnas.96.7.4204 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 182PT UT WOS:000079507900153 PM 10097188 ER PT J AU Geller, N Freedman, L Lee, YJ DerSimonian, R AF Geller, N Freedman, L Lee, YJ DerSimonian, R TI Conference on meta-analysis in the design and monitoring of clinical trials SO STATISTICS IN MEDICINE LA English DT Letter ID PREECLAMPSIA; CALCIUM C1 Natl Inst Child Hlth & Dev, Div Epidemiol Stat & Prevent Res, Bethesda, MD 20892 USA. RP Geller, N (reprint author), Natl Inst Child Hlth & Dev, Div Epidemiol Stat & Prevent Res, Execut Bldg 7B13P,6100 Execut Blvd MSC 7510, Bethesda, MD 20892 USA. NR 3 TC 2 Z9 2 U1 0 U2 2 PU JOHN WILEY & SONS LTD PI W SUSSEX PA BAFFINS LANE CHICHESTER, W SUSSEX PO19 1UD, ENGLAND SN 0277-6715 J9 STAT MED JI Stat. Med. PD MAR 30 PY 1999 VL 18 IS 6 BP 753 EP 754 PG 2 WC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Medicine, Research & Experimental; Statistics & Probability SC Mathematical & Computational Biology; Public, Environmental & Occupational Health; Medical Informatics; Research & Experimental Medicine; Mathematics GA 177FB UT WOS:000079198900009 PM 10204202 ER PT J AU Kirkwood, CD Gentsch, JR Hoshino, Y Clark, HF Glass, RI AF Kirkwood, CD Gentsch, JR Hoshino, Y Clark, HF Glass, RI TI Genetic and antigenic characterization of a serotype P[6]G9 human rotavirus strain isolated in the United States SO VIROLOGY LA English DT Article ID POLYMERASE CHAIN-REACTION; MONOCLONAL-ANTIBODIES; SEQUENCE-ANALYSIS; NEUTRALIZATION EPITOPES; VP4; IDENTIFICATION; DIVERSITY; VACCINES; CHILDREN; SITES AB During an epidemiologic survey of rotavirus infections established to monitor the prevalent G serotypes circulating in the United States, human P[6]G9, subgroup I rotavirus strains causing symptomatic infections were identified as the fourth most common serotype. In this report we describe the molecular and antigenic characterization of one of these P[6]G9 isolates (US1205). Neutralization and sequencing studies have demonstrated that both outer capsid proteins, VP7 and VP4, of US1205 are closely related to but genetically and antigenically distinguishable from those of standard G9 strains (e.g., F45, WI61) and standard P2A[6] strains (e.g., ST3, M37). Thus the complete antigenic type of US1205 is P2A[6]G9, subgroup I. Sequence analysis of the VP6 and NSP4 genes of US1205;indicates that strain US1205 possessed VP6 subgroup I and NSP4A genotype specificities. Finally, Northern hybridization studies suggest that the P[6]G9 strains are closely related to members of the DS-I genogroup except for their P[6] VP4 gene, which has been commonly identified in strains of both major human genogroups, and their G9 VP7 gene, which may have been derived by reassortment with a Wa genogroup strain. Examination of historic collections and prospective surveillance of strains will he needed to determine whether this strain has been present for some time or if it is emerging to compete with the other common serotypes of rotavirus, (C) 1999 Academic Press. C1 Ctr Dis Control & Prevent, Viral Gastroenteritis Sect, Resp & Enter Viruses Branch, Div Viral & Rickettsial Dis,Natl Ctr Infect Dis, Atlanta, GA 30333 USA. NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. Childrens Hosp Philadelphia, Philadelphia, PA 19104 USA. RP Kirkwood, CD (reprint author), Ctr Dis Control & Prevent, Viral Gastroenteritis Sect, Resp & Enter Viruses Branch, Div Viral & Rickettsial Dis,Natl Ctr Infect Dis, MS G-04,1600 Clifton Rd NE, Atlanta, GA 30333 USA. EM cek4@cdc.gov NR 42 TC 25 Z9 27 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAR 30 PY 1999 VL 256 IS 1 BP 45 EP 53 DI 10.1006/viro.1998.9591 PG 9 WC Virology SC Virology GA 184MK UT WOS:000079615800006 PM 10087225 ER PT J AU Gorelick, RJ Gagliardi, TD Bosche, WJ Wiltrout, TA Coren, LV Chabot, DJ Lifson, JD Henderson, LE Arthur, AO AF Gorelick, RJ Gagliardi, TD Bosche, WJ Wiltrout, TA Coren, LV Chabot, DJ Lifson, JD Henderson, LE Arthur, AO TI Strict conservation of the retroviral nucleocapsid protein zinc finger is strongly influenced by its role in viral infection processes: Characterization of HIV-1 particles containing mutant nucleocapsid zinc-coordinating sequences SO VIROLOGY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; MURINE LEUKEMIA-VIRUS; POLYMERASE CHAIN-REACTION; RNA ANNEALING ACTIVITIES; CYS-HIS BOX; IN-VITRO; STRAND TRANSFER; GENOMIC RNA; REVERSE TRANSCRIPTION; DNA-SYNTHESIS AB The retroviral nucleocapsid (NC) protein contains highly conserved amino acid sequences (-Cys-X-2-Cys-X-4-His-X-4-Cys-) designated retroviral (CCHC) Zn2+ fingers. The NC protein of murine leukemia viruses contains one NC Zn2+ finger and mutants that were competent in metal binding (CCCC and CCHH) packaged wild-type levels of full-length viral RNA but were not infectious. These studies were extended to human immunodeficiency virus type 1 (HIV-1), a virus with two NC Zn2+ fingers. Viruses with combinations of CCHC, CCCC, and CCHH Zn2+ fingers in each position of HIV-I NC were characterized. Mutant particles contained the normal complement of processed viral proteins. Four mutants packaged roughly wild-type levels of genomic RNA, whereas the remaining mutants packaged reduced levels. Virions with mutated C-terminal position NC fingers were replication competent One interesting mutant, containing a CCCC Zn2+ finger in the N-terminal position of NC, packaged wild-type levels of viral RNA and showed similar to 5% wild-type levels of infectivity when examined in CD4-expressing HeLa cells containing an HIV-1 LTR/beta-galactosidase construct. However, this particular mutant was replication defective in H9 cells; all other mutants were replication defective over the 8-week course of the assay. Two long terminal repeat viral DNA species could be detected in the CCCC mutant but not in any of the other replication-defective mutants. These studies show that the N-terminal Zn2+ finger position is more sensitive to alterations than the C-terminal position with respect to replication. Additionally, the retroviral (CCHC) NC Zn2+ finger is required for early infection processes. The evolutionary pressure to maintain CCHC NC Zn2+ fingers depends mainly on its function in infection processes, in addition to its function in genome packaging. C1 NCI, Frederick Canc Res & Dev Ctr, AIDS Vaccine Program, SAIC Frederick, Frederick, MD 21702 USA. RP NCI, Frederick Canc Res & Dev Ctr, AIDS Vaccine Program, SAIC Frederick, Frederick, MD 21702 USA. EM gorelick@avpaxp1.ncifcrf.gov FU NCI NIH HHS [N01-CO-56000] NR 50 TC 107 Z9 108 U1 0 U2 1 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAR 30 PY 1999 VL 256 IS 1 BP 92 EP 104 DI 10.1006/viro.1999.9629 PG 13 WC Virology SC Virology GA 184MK UT WOS:000079615800011 PM 10087230 ER PT J AU Hampel, H Teipel, SJ Padberg, F Haslinger, A Riemenschneider, M Schwarz, MJ Kotter, HU Scheloske, M Buch, K Stubner, S Dukoff, R Lasser, R Muller, N Sunderland, T Rapoport, SI Moller, HJ AF Hampel, H Teipel, SJ Padberg, F Haslinger, A Riemenschneider, M Schwarz, MJ Kotter, HU Scheloske, M Buch, K Stubner, S Dukoff, R Lasser, R Muller, N Sunderland, T Rapoport, SI Moller, HJ TI Discriminant power of combined cerebrospinal fluid tau protein and of the soluble interleukin-6 receptor complex in the diagnosis of Alzheimer's disease SO BRAIN RESEARCH LA English DT Article; Proceedings Paper CT 6th International Conference on Alzheimers Disease and Related Disorders CY JUL 18-23, 1998 CL AMSTERDAM, NETHERLANDS DE Alzheimer's disease; diagnosis; CSF; tau; gp130; interleukin-6 receptor complex; soluble receptor; biological marker; ELISA; discriminant analysis; jackknife procedure ID PAIRED HELICAL FILAMENTS; IL-6 RECEPTOR; GP130; DEMENTIA; CYTOKINES; COMPONENT; FAMILY; CELLS; IMMUNOREACTIVITY; DEGENERATION AB Alzheimer's disease (AD) still can only be definitively diagnosed with certainty by examination of brain tissue. There is a great need for a noninvasive, sensitive and specific in vivo test for AD. We combined cerebrospinal fluid analyses of tau protein (levels were significantly increased in AD patients [p = 0.0001]), a putative marker of neuronal degeneration, with components of the soluble interleukin-6 receptor complex (sIL-6RC: IL-6, soluble IL-6 receptor and soluble gp130), putative markers of neuroregulatory and inflammatory processes in the brain. A stepwise multivariate discriminant analysis revealed that tau protein and soluble gp130 (levels were significantly reduced in AD subjects [p = 0.007]), the affinity converting and signal-transducing receptor of neuropoietic cytokines, maximized separation between the investigated groups. The discriminant function predicted 23 of 25 clinically diagnosed AD patients (sensitivity 92%) with mild to moderate dementia correctly as having AD. Furthermore, 17 of 19 physically and cognitively healthy age-matched control subjects (specificity 90%) were accurately distinguished by this test, Later predicting with the jackknife procedure each case in turn through the remaining patient group, the discriminant function remained stable. Our data suggest that multivariate discriminant analysis of combined CSF tau protein and sIL-6RC components may add more certainty to the diagnosis of AD, however, the method will need to be extended to an independent group of patients, comparisons and control subjects to assess the true applicability. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Univ Munich, Dept Psychiat, Geriatr Psychiat Branch, Dementia Res Sect, D-80336 Munich, Germany. Tech Univ Munich, Dept Psychiat, D-81675 Munich, Germany. NIMH, NIH, Geriatr Psychiat Branch, Bethesda, MD 20892 USA. NIA, NIH, Neurosci Lab, Bethesda, MD 20892 USA. RP Hampel, H (reprint author), Univ Munich, Dept Psychiat, Geriatr Psychiat Branch, Dementia Res Sect, Nussbaumstr 7, D-80336 Munich, Germany. EM hampel@psy.med.uni-muenchen.de NR 75 TC 63 Z9 63 U1 0 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 27 PY 1999 VL 823 IS 1-2 BP 104 EP 112 DI 10.1016/S0006-8993(99)01146-4 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 179QP UT WOS:000079340400012 PM 10095017 ER PT J AU Rende, M Morales, M Brizi, E Bruno, R Bloom, F Sanna, PP AF Rende, M Morales, M Brizi, E Bruno, R Bloom, F Sanna, PP TI Modulation of serotonin 5-HT3 receptor expression in injured adult rat spinal cord motoneurons SO BRAIN RESEARCH LA English DT Article DE serotonin; motoneuron; regeneration; axotomy; neurotrophin; receptor; sciatic nerve; peripheral nerve ID GROWTH-FACTOR RECEPTOR; MESSENGER-RNA; NERVE LESIONS; IN-VIVO; RELEASE; NEURONS; IMMUNOREACTIVITY; DOPAMINE; AXOTOMY; SLICES AB The effects of sciatic nerve lesions on the expression of serotonin 5-HT3 receptor (5-HT3R) alpha subunit in motoneurons of the spinal cord was investigated by semi-quantitative immunohistochemistry. Following sciatic nerve crush, a significant reduction in density of staining in motoneurons was observed in longitudinal sections of the ventral horn at 3 and 15 days on the lesioned side when compared to the contralateral side (p < 0.01). At 30 days after crush, after completion of sciatic nerve regeneration and reinnervation of peripheral targets, intensity of staining had returned to normal. Conversely, after sciatic nerve cut, a lesion that does not allow for target reinnervation, highly significant reductions were observed at 3, 15, 30 and 45 days. These results suggest a rob for functional contacts with muscular targets in the maintenance of 5-HT3R expression in spinal motoneurons. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA. Univ Calabria, Dept Cell Biol, I-87036 Cosenza, Italy. Univ Perugia, Sch Med, Dept Expt Med & Biochem Sci, I-06100 Perugia, Italy. NIDA, Baltimore, MD USA. RP Sanna, PP (reprint author), Scripps Res Inst, Dept Neuropharmacol, La Jolla, CA 92037 USA. FU Telethon [589] NR 25 TC 4 Z9 4 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 27 PY 1999 VL 823 IS 1-2 BP 234 EP 240 DI 10.1016/S0006-8993(99)01180-4 PG 7 WC Neurosciences SC Neurosciences & Neurology GA 179QP UT WOS:000079340400030 PM 10095035 ER PT J AU Thomas, FT Ricordi, C Contreras, JL Hubbard, WJ Jiang, XL Eckhoff, DE Cartner, S Bilbao, G Neville, DM Thomas, JM AF Thomas, FT Ricordi, C Contreras, JL Hubbard, WJ Jiang, XL Eckhoff, DE Cartner, S Bilbao, G Neville, DM Thomas, JM TI Reversal of naturally occurring diabetes in primates by unmodified islet xenografts without chronic immunosuppression SO TRANSPLANTATION LA English DT Article ID PANCREATIC-ISLETS; RHESUS-MONKEY; TRANSPLANTATION; ALLOGRAFTS; RECIPIENTS; INDUCTION; TOLERANCE; MELLITUS; ANTI-CD3-IMMUNOTOXIN; PERITRANSPLANT AB Background. Isolated pancreatic islet transplantation (IPITx) is an attractive alternative for treatment of insulin-dependent diabetes mellitus (IDDM). How-ever, IPITx has been difficult to implement clinically because islets frequently fail to function, have a high incidence of rejection, and are susceptible to autoimmune recurrence and damage by chronic immunosuppressive therapy. Tolerance induction may be a rational approach to resolve several of these limitations. Because anti-CD3 immunotoxin (IT) has been successful in promoting stable primate kidney transplant tolerance in our experience, we considered that tolerance induction with IT might be duplicated in IPITx. Materials and Methods. Three monkeys with spontaneous IDDM (two Macaca fascicularis and one Ceropithecus aethiops) were treated with xenogeneic pancreatic islets (Macaca mulatta). Intrahepatic islet transplantation was performed at a mean of 13136+/-3860 islet equivalents/kg. Islet xenograft acceptance was accomplished by tolerance induction with two injections of IT given on day 0 at 2 hr before transplantation and on day +1, respectively. IT treatment was supplemented with cyclosporine and steroids administered on days 0 through 4. No additional immunosuppression was given thereafter. Two additional control macaques with spontaneous IDDM received the immunosuppressive protocol without islet infusion. Results, All recipients were restored to stable euglycemia, off exogenous insulin, within 1-2 weeks after transplantation. Glucose tolerance, C-peptide, and glycosylated hemoglobin tests confirmed the restoration of normal glucose homeostasis after islet transplantation. All three islet recipients have remained euglycemic at 410, 255, and 100 days of follow-up despite recovery of peripheral T cells to normal levels. In contrast, none of the controls presented changes in the diabetic status 4 and 8 months after treatment. Conclusions. These results represent the first demonstration in nonhuman primates of stable, long-term acceptance of nonencapsulated xenogeneic islets off all immunosuppression, suggesting operational tolerance. The findings have potential implications for islet transplantation as well as improved and more cost-effective therapy for IDDM. C1 Univ Alabama, Dept Surg, Div Transplantat Immunol, Birmingham, AL 35294 USA. Univ Alabama, Dept Comparat Med, Birmingham, AL 35294 USA. Univ Alabama, Gene Therapy Program, Birmingham, AL 35294 USA. Univ Miami, Diabet Res Inst, Miami, FL 33136 USA. NIMH, Mol Biol Lab, Bethesda, MD 20800 USA. RP Thomas, JM (reprint author), Univ Alabama, Dept Surg, Div Transplantat Immunol, BDB 802,1808 7th Ave S, Birmingham, AL 35294 USA. OI Ricordi, Camillo/0000-0001-8092-7153; Cartner, Samuel/0000-0002-2200-2627 FU NIAID NIH HHS [AI 39793, R01 AI 122293] NR 36 TC 63 Z9 63 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0041-1337 J9 TRANSPLANTATION JI Transplantation PD MAR 27 PY 1999 VL 67 IS 6 BP 846 EP 854 DI 10.1097/00007890-199903270-00011 PG 9 WC Immunology; Surgery; Transplantation SC Immunology; Surgery; Transplantation GA 183MA UT WOS:000079556300011 PM 10199733 ER PT J AU Von Lubitz, DKJE Lin, RCS Bischofberger, N Beenhakker, M Boyd, M Lipartowska, R Jacobson, KA AF Von Lubitz, DKJE Lin, RCS Bischofberger, N Beenhakker, M Boyd, M Lipartowska, R Jacobson, KA TI Protection against ischemic damage by adenosine amine congener, a potent and selective adenosine A(1) receptor agonist SO EUROPEAN JOURNAL OF PHARMACOLOGY LA English DT Article DE cerebral ischemia; adenosine A(1) receptor; therapy; gerbil ID ADENYLATE CYCLASE SYSTEM; CEREBRAL-ISCHEMIA; DESENSITIZATION; ANTAGONIST AB Although the selectivity and potency of adenosine amine congener (ADAC) at adenosine A(1) receptors are similar to other highly selective agonists at this receptor type, the chemical structure of the N-6 substituent is completely different. We now demonstrate that the characteristics of the therapeutic profile of ADAC are distinct from those observed during our previous studies of adenosine A(1) receptor agonist-mediated neuroprotection. Most significantly, chronic treatment with low microgram doses of ADAC (25-100 mu g/kg) protects against both mortality and neuronal damage induced by 10 min bilateral carotid occlusion in gerbils. At higher chronic doses, the statistical significance of the protective effect is lost. Acute preischemic administration of the drug at 75-200 mu g/kg also results in a statistically significant reduction of postischemic mortality and morbidity. These data indicate that, contrary to other adenosine A(1) receptor agonists whose chronic administration enhances postocclusive brain damage, ADAC may be a promising agent in treatment of both acute (e.g., cerebral ischemia) and chronic (seizures) disorders of the central nervous system in which adenosine A(1) receptors appear to be involved. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Univ Michigan, Sect Emergency Med, Emergency Med Res Labs, Ann Arbor, MI 48109 USA. Hahnemann Univ, Dept Anat & Neurobiol, Philadelphia, PA 19102 USA. Gilead Sci Inc, Foster City, CA 94404 USA. NIH, Mol Recognit Sect, Bioorgan Chem Lab, NIDDK, Bethesda, MD 20892 USA. Sch Med, Dept Pharmacol, Bialystok, Poland. RP Von Lubitz, DKJE (reprint author), Univ Michigan, Sect Emergency Med, Emergency Med Res Labs, 1500 Med Ctr Dr,UH-B1C255-0014, Ann Arbor, MI 48109 USA. EM ramillies@worldnet.att.net RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z99 DK999999, Z01 DK031117-20] NR 26 TC 41 Z9 42 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-2999 J9 EUR J PHARMACOL JI Eur. J. Pharmacol. PD MAR 26 PY 1999 VL 369 IS 3 BP 313 EP 317 DI 10.1016/S0014-2999(99)00073-4 PG 5 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 183FD UT WOS:000079542800008 PM 10225368 ER PT J AU Doering, T Proia, RL Sandhoff, K AF Doering, T Proia, RL Sandhoff, K TI Accumulation of protein-bound epidermal glucosylceramides in beta-glucocerebrosidase deficient type 2 Gaucher mice SO FEBS LETTERS LA English DT Article DE glucocerebrosidase; Gaucher disease; sphingolipid; epidermal permeability barrier; knockout mouse ID DISEASE; CERAMIDES AB The epidermal permeability barrier for water is essentially maintained by extracellular lipid membranes within the interstices of the stratum corneum. Ceramides, the main components of these membranes, derive in large part from hydrolysis of glucosylceramides mediated by the lysosomal enzyme beta-glucocerebrosidase. As analyzed in this work, the beta-glucocerebrosidase deficiency in type 2 Gaucher mice (RecNci I) resulted in an accumulation of all epidermal glucosylceramide species accompanied with a decrease of the related ceramides, However, the levels of one ceramide subtype, which possesses an alpha-hydroxypalmitic acid, was not altered in RecNci I mice suggesting that the beta-glucocerebrosidase pathway is not required for targeting of this lipid to interstices of the stratum corneum, Most importantly, omega-hydroxylated glucosylceramides which are protein-bound to the epidermal cornified cell envelope of the transgenic mice accumulated up to 35-fold whereas levels of related protein-bound ceramides and fatty acids were decreased to 100% of normal control. These data support the hypothesis that in wild-type epidermis omega-hydroxylated glucosylceramides are first transferred enzymatically from their linoleic esters to proteins of the epidermal cornified cell envelope and then catabolized to protein-bound ceramides and fatty acids, thus contributing at least in part to the formation of the lipid-bound envelope. (C) 1999 Federation of European Biochemical Societies. C1 Univ Bonn, Kekule Inst Organ Chem & Biochem, D-53121 Bonn, Germany. NIDDKD, Genet Dev & Dis Branch, NIH, Bethesda, MD 20892 USA. RP Sandhoff, K (reprint author), Univ Bonn, Kekule Inst Organ Chem & Biochem, Gerhard Domagk Str 1, D-53121 Bonn, Germany. RI Proia, Richard/A-7908-2012 NR 16 TC 64 Z9 65 U1 2 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0014-5793 J9 FEBS LETT JI FEBS Lett. PD MAR 26 PY 1999 VL 447 IS 2-3 BP 167 EP 170 DI 10.1016/S0014-5793(99)00274-4 PG 4 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 183NZ UT WOS:000079560800009 PM 10214939 ER PT J AU Pourquier, P Ueng, LM Fertala, J Wang, D Park, HJ Essigmann, JM Bjornsti, MA Pommier, Y AF Pourquier, P Ueng, LM Fertala, J Wang, D Park, HJ Essigmann, JM Bjornsti, MA Pommier, Y TI Induction of reversible complexes between eukaryotic DNA topoisomerase I and DNA-containing oxidative base damages - 7,8-dihydro-8-oxoguanine and 5-hydroxycytosine SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID ACTIVE-SITE TYROSINE; SACCHAROMYCES-CEREVISIAE; IONIZING-RADIATION; ESCHERICHIA-COLI; ENDONUCLEASE-III; ABASIC SITES; CAMPTOTHECIN; CLEAVAGE; REPAIR; 8-HYDROXYGUANINE AB We recently showed that abasic sites, uracil mismatches, nicks, and gaps can trap DNA topoisomerase I (top1) when these lesions are introduced in the vicinity of a top1 cleavage site (Pourquier, P., Ueng, L.-M., Kohlhagen, G., Mazumder, A, Gupta, Ri., Kohn, K. W., and Pommier, Y, (1997) J. Biol. Chem. 272, 7792-7796; Pourquier, P., Pilon, A. A, Kohlhagen, G., Mazumder, A., Sharma, A, and Pommier, Y. (1997) J. Biol. Chem. 26441-26447). In this study, we investigated the effects on top1 of an abundant base damage generated by various oxidative stresses: 7,8-dihydro-8-oxoguanine (8-oxoG). Using purified eukaryotic top1 and oligonucleotides containing the 8-oxoG modification, we found a 3-7-fold increase in top1-mediated DNA cleavage when 8-oxoG was present at the +1 or +2 position relative to the cleavage site. Another oxidative lesion, 8-hydroxycytosine, also enhanced top1 cleavage by a-fold when incorporated at the +1 position of the scissile strand. 8-oxoG at the +1 position enhanced noncovalent top1 DNA binding and had no detectable effect on DNA religation or on the incision step. top1 trapping by 8-oxoG was markedly enhanced when asparagine adjacent to the catalytic tyrosine was mutated to histidine, suggesting a direct interaction between this residue and the DNA major groove immediately downstream from the top1 cleavage site. Altogether, these results demonstrate that oxidative base lesions can increase top1 binding to DNA and induce top1 cleavage complexes. C1 NCI, Mol Pharmacol Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. Thomas Jefferson Univ, Dept Biochem & Mol Pharmacol, Philadelphia, PA 19107 USA. MIT, Dept Chem, Cambridge, MA 02139 USA. RP Pommier, Y (reprint author), NCI, Mol Pharmacol Lab, Div Basic Sci, NIH, Bldg 37,Rm 5D02, Bethesda, MD 20892 USA. EM pommier@nih.gov FU NCI NIH HHS [CA52127, CA58755] NR 57 TC 134 Z9 135 U1 1 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 1999 VL 274 IS 13 BP 8516 EP 8523 DI 10.1074/jbc.274.13.8516 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 181PV UT WOS:000079451600026 PM 10085084 ER PT J AU Bianco, C Kannan, S De Santis, M Seno, M Tang, CK Martinez-Lacaci, I Kim, N Wallace-Jones, B Lippman, ME Ebert, AD Wechselberger, C Salomon, DS AF Bianco, C Kannan, S De Santis, M Seno, M Tang, CK Martinez-Lacaci, I Kim, N Wallace-Jones, B Lippman, ME Ebert, AD Wechselberger, C Salomon, DS TI Cripto-1 indirectly stimulates the tyrosine phosphorylation of erb B-4 through a novel receptor SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID NEU DIFFERENTIATION FACTOR; MAMMARY EPITHELIAL-CELLS; GROWTH-FACTOR RECEPTOR; EGF RECEPTOR; SIGNALING NETWORK; PROTEIN; GENE; ACTIVATION; EXPRESSION; FAMILY AB Cripto-1 (CR-1) is a recently discovered protein of the epidermal growth factor family that fails to directly bind to any of the four known erb B type 1 receptor tyrosine kinases. The present study demonstrates that CR-1 indirectly induces tyrosine phosphorylation of erb B-4 but not of the epidermal growth factor-related receptors erb B-2 and erb B-3 in different mouse and human mammary epithelial cell lines. In addition, downregulation of erb B-4 in NMuMG mouse mammary epithelial cells and in T47D human breast cancer cells, using an anti-erb B-4 blocking antibody or a hammerhead ribozyme vector targeted to erb B-4 mRNA, impairs the ability of CR-1 to fully activate mitogen-activated protein kinase. Finally, chemical cross-linking of I-125-CR-1 to mouse and human mammary epithelial cell membranes results in the labeling of two specific bands with a molecular weight of 130 and 60 kDa, suggesting that the CR-1 receptor represents a novel receptor structurally unrelated to any of the known type I receptor tyrosine kinases. In conclusion, these data demonstrate that CR-1, upon binding to an unknown receptor, can enhance the tyrosine kinase activity of erb B-4 and that a functional erb B-4 receptor is required for CR-1-induced MAPK activation. C1 NCI, Tumor Growth Factor Sect, Lab Tumor Immunol & Biol, NIH, Bethesda, MD 20892 USA. Macmaster Univ, Hamilton, ON L8S 4K1, Canada. Okayama Univ, Fac Engn, Dept Biosci & Biotechnol, Okayama 7008530, Japan. Georgetown Univ, Med Ctr, Vincent T Lombardi Canc Res Ctr, Dept Biochem, Washington, DC 20007 USA. RP Salomon, DS (reprint author), NCI, Tumor Growth Factor Sect, Lab Tumor Immunol & Biol, NIH, Bldg 10,Room 5B39, Bethesda, MD 20892 USA. EM davetgfa@helix.nih.gov NR 39 TC 58 Z9 58 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 1999 VL 274 IS 13 BP 8624 EP 8629 DI 10.1074/jbc.274.13.8624 PG 6 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 181PV UT WOS:000079451600041 PM 10085099 ER PT J AU Qi, HY Bernstein, HD AF Qi, HY Bernstein, HD TI SecA is required for the insertion of inner membrane proteins targeted by the Escherichia coli signal recognition particle SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID CYTOPLASMIC MEMBRANE; PLASMA-MEMBRANE; ER MEMBRANE; 4.5S RNA; TRANSLOCATION; EXPORT; SECRETION; GENE; ATP; RIBONUCLEOPROTEIN AB Recent work has demonstrated that the signal recognition particle (SRP) is required for the efficient insertion of many proteins into the Escherichia coli inner membrane (IM), Based on an analogy to eukaryotic SRP, it is likely that bacterial SRP binds to inner membrane proteins (IMPs) co-translationally and then targets them to protein transport channels ("translocons"), Here we present evidence that SecA, which has previously been shown to facilitate the export of proteins targeted in a post-translational fashion, is also required for the membrane insertion of proteins targeted by SRP. The introduction of SecA mutations into strains that have modest SRP deficiencies produced a synthetic lethal effect, suggesting that SecA and SRP might function in the same biochemical pathway. Consistent with this explanation, depletion of SecA by inactivating a temperature-sensitive amber suppressor in a secA(am) strain completely blocked the membrane insertion of AcrB, a protein that is targeted by SRP. In the absence of substantial SecA, pulse-labeled AcrB was retained in the cytoplasm even after a prolonged chase period and was eventually degraded. Although protein export was also severely impaired by SecA depletion, the observation that more than 20% of the OmpA molecules were translocated properly showed that translocons were still active. Taken together, these results imply that SecA plays a much broader role in the transport of proteins across the E. coli IM than has been previously recognized. C1 NIDDK, Genet & Biochem Branch, NIH, Bethesda, MD 20892 USA. RP Bernstein, HD (reprint author), NIDDK, Genet & Biochem Branch, NIH, Bldg 10,Rm 9D-20, Bethesda, MD 20892 USA. EM harris_bernstein@nih.gov NR 38 TC 68 Z9 68 U1 0 U2 5 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 1999 VL 274 IS 13 BP 8993 EP 8997 DI 10.1074/jbc.274.13.8993 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 181PV UT WOS:000079451600088 PM 10085146 ER PT J AU Coon, SL Begay, V Deurloo, D Falcon, J Klein, DC AF Coon, SL Begay, V Deurloo, D Falcon, J Klein, DC TI Two arylalkylamine N-acetyltransferase genes mediate melatonin synthesis in fish SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID PINEAL-GLAND; MESSENGER-RNA; CIRCADIAN REGULATION; RETINA; RHYTHM; EXPRESSION; METABOLISM; CLONING; ENZYME; ORGAN AB Serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase, AANAT, EC 2.3.1.87) is the first enzyme in the conversion of serotonin to melatonin, Large changes in AANAT activity play an important role in the daily rhythms in melatonin production. Although a single AANAT gene has been found in mammals and the chicken, we have now identified two AANAT genes in fish. These genes are designated AANAT-1 and AANAT-2; all known AANATs belong to the AANAT-1 subfamily. PikeAANAT-1 is nearly exclusively expressed in the retina and AANAT-2 in the pineal gland. The abundance of each mRNA changes on a circadian basis, with retinal AANAT-1 mRNA peaking in late afternoon and pineal AANAT-2 mRNA peaking 6 h later. The pike AANAT-1 and AANAT-2 enzymes (66% identical amino acids) exhibit marked differences in their affinity for serotonin, relative affinity for indoleethylamines versus phenylethylamines and temperature-activity relationships. Two AANAT genes also exist in another fish, the trout. The evolution of two AANATs may represent a strategy to optimally meet tissue-related requirements for synthesis of melatonin: pineal melatonin serves an endocrine role and retinal melatonin plays a paracrine role. C1 NICHD, Sect Neuroendocrinol, Dev Neurobiol Lab, NIH, Bethesda, MD 20892 USA. Lab Neurobiol Cellulaire, Dept Neurosci, CNRS, UMR 6558, F-86022 Poitiers, France. RP Klein, DC (reprint author), NICHD, Sect Neuroendocrinol, Dev Neurobiol Lab, NIH, Bldg 49,Rm 6A-82, Bethesda, MD 20892 USA. EM klein@helix.nih.gov RI FALCON, Jack/I-5302-2013 OI FALCON, Jack/0000-0002-7572-6581 NR 35 TC 71 Z9 74 U1 0 U2 3 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 26 PY 1999 VL 274 IS 13 BP 9076 EP 9082 DI 10.1074/jbc.274.13.9076 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 181PV UT WOS:000079451600099 PM 10085157 ER PT J AU Gong, L Wyatt, RJ Baker, I Masserano, JM AF Gong, L Wyatt, RJ Baker, I Masserano, JM TI Brain-derived and glial cell line-derived neurotrophic factors protect a catecholaminergic cell line from dopamine-induced cell death SO NEUROSCIENCE LETTERS LA English DT Article DE dopamine; cell death; glutathione; superoxide dismutase; brain-derived neurotrophic factor; glial cell line-derived neurotrophic factor; CATH.a cells ID SUPEROXIDE-DISMUTASE; PARKINSONS-DISEASE; NEURONAL CELLS; IN-VIVO; GLUTATHIONE; TOXICITY; SYSTEM; SURVIVAL; RATS AB Brain-derived neurotrophic factor (BDNF) promotes the survival of dopaminergic neurons in primary cultures and protects these neurons from the neurotoxic effects of 6-hydroxydopamine. The protective mechanism of BDNF on neurotoxicity was evaluated using CATH.a cells, a clonal catecholaminergic cell line derived from the central nervous system. Dopamine produced a dose-dependent cell death in CATH.a cells. Treatment of CATH.a cells with BDNF or glia cell line-derived neurotrophic factor (GDNF) reduced dopamine-induced cell death by approximately 60-70%. Nerve growth factor, basic fibroblast growth factor, neurotrophin-4/5 and insulin had no protective effect on dopamine-induced cell death. Dopamine decreased the activity of superoxide dismutase and the levels of glutathione in the CATH.a cells and these decreases were reversed by BDNF. In addition, BDNF treatment alone increased superoxide dismutase activity by 108%, These results suggest that BDNF may safeguard CATH.a cells from dopamine-induced cell death by maintaining or enhancing components of the cell, which protect from oxidative stress. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved. C1 NIMH, Neuropsychiat Branch, Bethesda, MD 20892 USA. RP Masserano, JM (reprint author), NIMH, Neuropsychiat Branch, 15 N Dr MSC 2668,Bldg 15-K,Room 201, Bethesda, MD 20892 USA. EM masseraj@intra.nimh.nih.gov NR 20 TC 33 Z9 35 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD MAR 26 PY 1999 VL 263 IS 2-3 BP 153 EP 156 DI 10.1016/S0304-3940(99)00148-2 PG 4 WC Neurosciences SC Neurosciences & Neurology GA 182NJ UT WOS:000079504800020 PM 10213158 ER PT J AU Anderson, JJ AF Anderson, JJ TI Interdisciplinary research at NIH SO SCIENCE LA English DT Letter C1 NIH, Div Genet & Dev Biol, Bethesda, MD 20892 USA. RP Anderson, JJ (reprint author), NIH, Div Genet & Dev Biol, Bldg 10, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD MAR 26 PY 1999 VL 283 IS 5410 BP 2018 EP 2018 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 180DH UT WOS:000079369800019 PM 10206904 ER PT J AU Wong, JP Yang, HM Nagata, L Kende, M Levy, H Schnell, G Blasetti, K AF Wong, JP Yang, HM Nagata, L Kende, M Levy, H Schnell, G Blasetti, K TI Liposome-mediated immunotherapy against respiratory influenza virus infection using double-stranded RNA poly ICLC SO VACCINE LA English DT Article; Proceedings Paper CT 1st World Congress on Vaccines and Immunization CY APR 26-30, 1998 CL ISTANBUL, TURKEY SP Infect Control World Org ID POLYRIBOCYTIDYLIC ACID COMPLEX; RHESUS-MONKEYS; MICE; INTERFERON; HYPOTHERMIA; FEVER AB The use of liposome delivery technology to enhance the antiviral activity of poly ICLC tan immunomodulating dsRNA) while decreasing its intrinsic toxicity is evaluated in this study. The antiviral efficacies of free and liposome-encapsulated poly ICLC were evaluated and compared using a lethal respiratory influenza A virus infection in mice. The toxicity profiles of free and liposome-encapsulated poly ICLC were compared by determining the extent of hypothermia and loss in body weights in mice pretreated with these drugs. Poly ICLC was encapsulated in cationic liposomes prepared by the freeze drying method, To determine the antiviral efficacies of free and liposome-encapsulated poly ICLC, mice were intranasally pretreated with two doses of poly ICLC (free or liposomal, 1 mg/kg/dose) given 48 h apart. Al various times post pretreatment mice were intranasally challenged with 10 LD50 mouse-adapted influenza A/PR/8 (H1N1) virus. The survival rates of the mice were determined at day 14 post infected and compared to the untreated control mice. Results indicate mice pretreated with liposome-encapsulated poly ICLC within 3 weeks prior to virus challenge were completely protected (100% survival compared to 0% for the untreated control group, p < 0.001), while window of protection provided by free unencapsulated poly ICLC was 12 days. When the toxicity profiles of free and liposome-encapsulated poly ICLC were compared, it was found that hypothermia and body weight loss induced by poly ICLC were either completely mitigated or significantly reduced in mice given equivalent doses of poly ICLC in the liposome-encapsulated form, These results suggest that liposomes are an excellent drug carrier for poly ICLC, that liposome-encapsulated poly ICLC may provide a safe and effective immunotherapeutic approach for the prevention of respiratory influenza virus infections. Published by Elsevier Science Ltd. C1 Def Res Estab Suffield, Med Countermeasures Sect, Medicine Hat, AB T1A 8K6, Canada. USA, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA. NIAID, Bethesda, MD 20892 USA. RP Wong, JP (reprint author), Def Res Estab Suffield, Med Countermeasures Sect, Box 4000, Medicine Hat, AB T1A 8K6, Canada. EM jonathan.wong@dres.dnd.ca NR 21 TC 28 Z9 31 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 26 PY 1999 VL 17 IS 13-14 SI SI BP 1788 EP 1795 DI 10.1016/S0264-410X(98)00439-3 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 178JM UT WOS:000079262600034 PM 10194841 ER PT J AU Khan, J Bittner, ML Chen, YD Meltzer, PS Trent, JM AF Khan, J Bittner, ML Chen, YD Meltzer, PS Trent, JM TI DNA microarray technology: the anticipated impact on the study of human disease SO BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER LA English DT Review DE DNA microarray technology; medical research; data uniformity; microarray ID DENSITY OLIGONUCLEOTIDE ARRAYS; HUMAN-GENOME-PROJECT; GENES; MAP C1 Natl Human Genome Res Inst, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. RP Trent, JM (reprint author), Natl Human Genome Res Inst, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. RI Khan, Javed/P-9157-2014 OI Khan, Javed/0000-0002-5858-0488 NR 19 TC 105 Z9 108 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-419X J9 BBA-REV CANCER JI Biochim. Biophys. Acta-Rev. Cancer PD MAR 25 PY 1999 VL 1423 IS 2 BP M17 EP M28 PG 12 WC Biochemistry & Molecular Biology; Biophysics; Oncology SC Biochemistry & Molecular Biology; Biophysics; Oncology GA 186ZY UT WOS:000079762500002 PM 10214349 ER PT J AU Aravind, L Neuwald, AF Ponting, CP AF Aravind, L Neuwald, AF Ponting, CP TI Sec14p-like domains in NF1 and Dbl-like proteins indicate lipid regulation of Ras and Rho signaling SO CURRENT BIOLOGY LA English DT Letter ID NUCLEOTIDE EXCHANGE FACTOR; NEUROFIBROMATOSIS TYPE-1; GENE; ONCOGENE; ENCODES; BINDS C1 NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. RP Aravind, L (reprint author), NIH, Natl Ctr Biotechnol Informat, Natl Lib Med, Bethesda, MD 20894 USA. FU NLM NIH HHS [R01 LM006747] NR 20 TC 42 Z9 44 U1 0 U2 1 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD MAR 25 PY 1999 VL 9 IS 6 BP R195 EP R197 DI 10.1016/S0960-9822(99)80127-4 PG 3 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 180RP UT WOS:000079399200006 PM 10209105 ER PT J AU Misteli, T AF Misteli, T TI RNA splicing: What has phosphorylation got to do with it? SO CURRENT BIOLOGY LA English DT Article ID PROTEIN AB Many pre-mRNA splicing factors are phosphorylated in vivo, but the role of this modification has been unclear. Recent observations suggest that phosphorylation modulates protein-protein interactions within the spliceosome, thereby contributing to dynamic structural reorganization of the spliceosome during splicing. C1 NCI, NIH, Bethesda, MD 20892 USA. RP Misteli, T (reprint author), NCI, NIH, Bethesda, MD 20892 USA. NR 12 TC 40 Z9 41 U1 0 U2 4 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD MAR 25 PY 1999 VL 9 IS 6 BP R198 EP + DI 10.1016/S0960-9822(99)80128-6 PG 4 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 180RP UT WOS:000079399200007 PM 10209090 ER PT J AU Wade, PA Wolffe, AP AF Wade, PA Wolffe, AP TI Transcriptional regulation: SWItching circuitry SO CURRENT BIOLOGY LA English DT Article ID EXPRESSION; YEAST; GENES AB Proteins of the SWI/SNF family disrupt chromatin, hydrolysing ATP in the process. How they do so is still mysterious, but recent studies indicate that they can be targeted to the nuclear infrastructure and to particular genes, where they cooperate with other enzymes to activate or repress transcription. C1 NICHHD, Mol Embryol Lab, NIH, Bethesda, MD 20892 USA. RP Wade, PA (reprint author), NICHHD, Mol Embryol Lab, NIH, Bldg 18T,Room 106, Bethesda, MD 20892 USA. NR 12 TC 29 Z9 29 U1 0 U2 0 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0960-9822 J9 CURR BIOL JI Curr. Biol. PD MAR 25 PY 1999 VL 9 IS 6 BP R221 EP + DI 10.1016/S0960-9822(99)80134-1 PG 5 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 180RP UT WOS:000079399200013 PM 10209086 ER PT J AU Ohe, K Ikuyama, S Takayanagi, R Kohn, LD Nawata, H AF Ohe, K Ikuyama, S Takayanagi, R Kohn, LD Nawata, H TI Nicotinamide potentiates TSHR and MHC class II promoter activity in FRTL-5 cells SO MOLECULAR AND CELLULAR ENDOCRINOLOGY LA English DT Article DE thyroid; nicotinamide; poly(ADP-ribose) polymerase; TSEP-1/YB-1; major histocompatibility complex class II; thyrotropin receptor ID THYROTROPIN RECEPTOR PROMOTER; TRANSCRIPTION FACTOR-I; HUMAN THYROID-CELLS; ANTISENSE RNA EXPRESSION; HLA-DR EXPRESSION; Y-BOX PROTEIN; GENE-EXPRESSION; POLY(ADP-RIBOSE) POLYMERASE; ANTIGEN EXPRESSION; RESPONSE ELEMENT AB Here we show that nicotinamide modulates the promoter activity of rat thyrotropin (TSHR) and major histocompatibility complex (MHC) class II genes in rat FRTL-5 thyroid cells, and have identified a novel mechanism for its action. TSHR and MHC class II, are potentiated through reduced expression of a common repressor of these two genes, TSEP-1 (TSHR suppressor element binding protein-1)/YB-1. Thus we show that TSHR mRNA is increased and TSHR promoter activity was concentration-dependently activated from 0 to 40 mM nicotinamide. The promoter lengths of TSHR and MHC class II containing TSEP/YB-1 binding sites were enhanced by 40 mM nicotinamide, but not the ones deleted of these binding sites. TSEP-1/YB-1 binding to the recognition sites in both TSHR and MHC class II promoters was reduced in nicotinamide-treated FRTL-5 nuclear extracts. Nicotinamide reduced the expression of TSEP-1/YB-1 mRNA and TSEP-1/YB-1 protein in the nucleus. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved. C1 Kyushu Univ, Med Inst Bioregulat, Dept Clin Immunol, Lab Mol Endocrinol & Metab, Beppu, Oita 8740838, Japan. Kyushu Univ, Fac Med, Dept Internal Med 3, Fukuoka 8128582, Japan. NIDDKD, Cell Regulat Sect, Metab Dis Branch, NIH, Bethesda, MD 20892 USA. RP Ikuyama, S (reprint author), Kyushu Univ, Med Inst Bioregulat, Dept Clin Immunol, Lab Mol Endocrinol & Metab, 4546 Tsurumihara, Beppu, Oita 8740838, Japan. RI U-ID, Kyushu/C-5291-2016 NR 55 TC 1 Z9 1 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0303-7207 J9 MOL CELL ENDOCRINOL JI Mol. Cell. Endocrinol. PD MAR 25 PY 1999 VL 149 IS 1-2 BP 141 EP 151 DI 10.1016/S0303-7207(98)00249-4 PG 11 WC Cell Biology; Endocrinology & Metabolism SC Cell Biology; Endocrinology & Metabolism GA 196NV UT WOS:000080315200015 PM 10375026 ER PT J AU Shaughnessy, JD Largaespada, DA Tian, EM Fletcher, CF Cho, BC Vyas, P Jenkins, NA Copeland, NG AF Shaughnessy, JD Largaespada, DA Tian, EM Fletcher, CF Cho, BC Vyas, P Jenkins, NA Copeland, NG TI Mrvil, a common MRV integration site in BXH2 myeloid leukemias, encodes a protein with homology to a lymphoid-restricted membrane protein Jaw1 SO ONCOGENE LA English DT Article DE myeloid leukemia model; retroviral insertional mutagenesis; common sites of integration ID C-MPL LIGAND; VIRUS; GENE; EXPRESSION; MICE; MEGAKARYOCYTOPOIESIS; CHROMOSOME-11; SEQUENCES; T(7-11)(P15-P15); TRANSLOCATION AB Ecotropic MuLVs induce myeloid leukemia in BXH2 mice by insertional mutagenesis of cellular protooncogenes or tumor suppressor genes, Disease genes can thus be identified by viral tagging as common sites of viral integration in BXH2 leukemias. Previous studies showed that a frequent common integration site in BXH2 leukemias is the NJ1 tumor suppressor gene. Unexpectedly, about half of the viral integrations at Nf1 represented a previously undiscovered defective nonecotropic virus, termed MRV, Because other common integration sites in BXH2 leukemias encoding protooncogenes contain ecotropic rather than MRV viruses, it has been speculated that MRV viruses may selectively target tumor suppressor genes. To determine if this were the case, 21 MRV-positive BXH2 leukemias were screened for new MRV common integration sites. One new site, Mrvi1 was identified that was disrupted by MRV in two of the leukemias. Ecotropic virus did not disrupt Mrvi1 in 205 ecotropic virus-positive leukemias, suggesting that Mrvi1 is specifically targeted by MRV, Mrvi1 encodes a novel protein with homology to Jaw1, a lymphoid restricted type II membrane protein that localizes to the endoplasmic reticulum, MRV integration occurs at the 5' end of the gene between two differentially used promoters. Within hematopoietic cells, Mrvi1 expression is restricted to megakaryocytes and some myeloid leukemias, Like Jaw1, which is downregulated during lymphoid differentiation, Mrv1 is do downregulated during monocytic differentiation of BXH2 leukemias, Taken together, these data suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation. Experiments are in progress to test whether Mrvi1 is a tumor suppressor gene. C1 Univ Arkansas Med Sci, Dept Med, Div Hematol & Oncol, Little Rock, AR 72205 USA. Univ Minnesota, Ctr Canc, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA. NCI, Mammalian Genet Lab, ABL Basic Res Program, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Thomas Jefferson Univ, Inst Canc, Dept Microbiol & Immunol, Philadelphia, PA 19107 USA. Childrens Hosp, Dept Hematol & Oncol, Boston, MA 02115 USA. RP Shaughnessy, JD (reprint author), Univ Arkansas Med Sci, Dept Med, Div Hematol & Oncol, Little Rock, AR 72205 USA. RI Largaespada, David/C-9832-2014 NR 47 TC 26 Z9 28 U1 0 U2 3 PU STOCKTON PRESS PI BASINGSTOKE PA HOUNDMILLS, BASINGSTOKE RG21 6XS, HAMPSHIRE, ENGLAND SN 0950-9232 J9 ONCOGENE JI Oncogene PD MAR 25 PY 1999 VL 18 IS 12 BP 2069 EP 2084 PG 16 WC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Oncology; Cell Biology; Genetics & Heredity GA 179TX UT WOS:000079346200004 PM 10321731 ER PT J AU Zhou, YF Yu, ZX Wanishsawad, C Shou, M Epstein, SE AF Zhou, YF Yu, ZX Wanishsawad, C Shou, M Epstein, SE TI The immediate early gene products of human cytomegalovirus increase vascular smooth muscle cell migration, proliferation, and expression of PDGF beta-receptor SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID MESSENGER-RNA EXPRESSION; GROWTH-FACTOR; BALLOON ANGIOPLASTY; ENDOTHELIAL-CELLS; ARTERIAL INJURY; INFECTION; ATHEROSCLEROSIS; RESTENOSIS; ASSOCIATION; INHIBITION AB Evidence suggests that human cytomegalovirus (HCMV) infection contributes to the development of atherosclerosis and restenosis. Because smooth muscle cell (SMC) proliferation and migration are crucial events of both processes, and because PDGF beta-receptor modulates SMC-migration, we determined-whether HCMV infection affects SMC proliferation, migration, and PDGF beta-receptor expression. We employed a SMC model in which HCMV:infection leads-to expression;of only the immediate early (IE)HCMV gene products-HCMV infection of rat SMCs. We found that HCMV infection significantly (i) increased SMC proliferation (from 0.9 x 10(6) +/- 0.024 x 10(6) to 1.4 x 10(6) +/- 0.051 x 10(6) cells/well, p < 0.001); (ii) augmented SMC migration toward PDGF (from 64 +/- 37 to 116 +/- 51 cells/high power field; p < 0.01); and (iii) enhanced PDGF beta-receptor expression in a time-dependent fashion. We conclude that HCMV infection of rat SMCs increases SMC proliferation, migration, and PDGF beta-receptor expression. These findings identify further mechanisms by which CMV may contribute to the development of-atherosclerosis and restenosis. (C) 1999 Academic Press. C1 Washington Hosp Ctr, Cardiovasc Res Fdn, Washington, DC 20010 USA. NHLBI, Cardiol Branch, NIH, Bethesda, MD 20892 USA. RP Zhou, YF (reprint author), Washington Hosp Ctr, Cardiovasc Res Fdn, Suite 4B-1,110 Irving St, Washington, DC 20010 USA. EM yfz1@mhg.edu NR 32 TC 78 Z9 83 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 24 PY 1999 VL 256 IS 3 BP 608 EP 613 DI 10.1006/bbrc.1999.0387 PG 6 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 182KW UT WOS:000079499000029 PM 10080946 ER PT J AU Grgurevich, S Mikhael, A McVicar, DW AF Grgurevich, S Mikhael, A McVicar, DW TI The Csk homologous kinase, Chk, binds tyrosine phosphorylated paxillin in human blastic T cells SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article DE kinase; Chk; Src; paxillin ID TERMINAL SRC KINASE; PROTEIN-KINASE; MOLECULAR-CLONING; SIGNAL-TRANSDUCTION; FAMILY KINASES; SH2 DOMAIN; IDENTIFICATION; ACTIVATION; MATK; GENE AB In determining the role of Chk in T cell signaling, we have focused on its protein-protein interactions. We detected a tyrosine phosphoprotein that coimmunoprecipitated with Chk from pervanadate stimulated human blastic T cells. Subsequent Western blot analysis identified this tyrosine phosphoprotein as paxillin. Paxillin, a cytoskeletal protein involved in focal adhesions, was first identified as a v-Src substrate in transformed fibroblasts. Interestingly, Chk specifically bound tyrosine phosphorylated paxillin. Consistent with our in vivo data, Chk and paxillin were observed to localize in similar cellular regions prior to and following stimulation. Using GST fusion proteins, we determined that the Chk SH2 domain, not the SH3 domain, bound tyrosine phosphorylated paxillin. Specifically, paxillin hound to the FLVRES motif of the Chk SH2 domain. Using Far Western analysis, we revealed that the Chk SH2 domain directly associates with tyrosine phosphorylated paxillin. Finally, p52(Chk) expression in Csk-deficient mouse embryo fibroblasts decreased total phosphotyrosine levels of paxillin, implying a physiological role for Chk. These studies provide important insight into the role of Chk in tyrosine mediated signaling, as well as T cell physiology. C1 NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Expt Immunol Lab, Frederick, MD 21702 USA. RP McVicar, DW (reprint author), NCI, Frederick Canc Res & Dev Ctr, Div Basic Sci, Expt Immunol Lab, Bldg 560,Rm 31-93, Frederick, MD 21702 USA. EM MCVICAR@NIH.GOV RI McVicar, Daniel/G-1970-2015 NR 57 TC 10 Z9 10 U1 0 U2 2 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 24 PY 1999 VL 256 IS 3 BP 668 EP 675 DI 10.1006/bbrc.1999.0398 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 182KW UT WOS:000079499000040 PM 10080957 ER PT J AU Katoh, E Yamazaki, T Kiso, Y Wingfield, PT Stahl, SJ Kaufman, JD Torchia, DA AF Katoh, E Yamazaki, T Kiso, Y Wingfield, PT Stahl, SJ Kaufman, JD Torchia, DA TI Determination of the rate of monomer interchange in a ligand-bound homodimeric protein from NOESY cross peaks: Application to the HIV protease/KNI-529 complex SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; KNI-272; NMR; INHIBITORS; POTENT C1 Natl Inst Agrobiol Resources, Struct Biol Unit, Tsukuba, Ibaraki 3058602, Japan. Kyoto Pharmaceut Univ, Dept Med Chem, Yamashima Ku, Kyoto 6078414, Japan. Natl Inst Dent & Craniofacial Res, Mol Struct Biol Unit, NIAMSD, Prot Express Lab,NIH, Bethesda, MD 20892 USA. RP Yamazaki, T (reprint author), Natl Inst Agrobiol Resources, Struct Biol Unit, Tsukuba, Ibaraki 3058602, Japan. NR 16 TC 11 Z9 11 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD MAR 24 PY 1999 VL 121 IS 11 BP 2607 EP 2608 DI 10.1021/ja984041v PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 180AU UT WOS:000079363300038 ER PT J AU Bahar, I Jernigan, RL AF Bahar, I Jernigan, RL TI Cooperative fluctuations and subunit communication in tryptophan synthase SO BIOCHEMISTRY LA English DT Article ID ALPHA(2)BETA(2) COMPLEX REVEALS; INDUCED CONFORMATIONAL-CHANGES; ALPHA-SUBUNIT; SALMONELLA-TYPHIMURIUM; ESCHERICHIA-COLI; FLEXIBLE LOOP; TRIOSEPHOSPHATE ISOMERASE; BIENZYME COMPLEX; BETA-SUBUNIT; INTERSUBUNIT COMMUNICATION AB Tryptophan synthase (TRPS), with linearly arrayed subunits alpha beta beta alpha, catalyzes the last two reactions in the biosynthesis of L-tryptophan. The two reactions take place in the respective alpha- and beta-subunits of the enzyme, and the intermediate product, indole, is transferred from the alpha- to the beta-site through a 25 Angstrom long hydrophobic tunnel. The occurrence of a unique ligand-mediated long-range cooperativity for substrate channeling, and a quest to understand the mechanism of allosteric control and coordination in metabolic cycles, have motivated many experimental studies on the structure and catalytic activity of the TRPS alpha(2)beta(2) complex and its mutants. The dynamics of these complexes are analyzed here using a simple but rigorous theoretical approach, the Gaussian network model, Both wild-typo and mutant structures, in the unliganded and various liganded forms, are considered. The substrate binding site in the beta-subunit is found to be closely coupled to a group of hinge residues (beta 77-beta 89 and beta 376-beta 379) near the beta-beta interface. These residues simultaneously control the anticorrelated motion of the two beta-subunits, and the opening or closing of the hydrophobic tunnel. The latter process is achieved by the large amplitude fluctuations of the so-called COMM domain in the same subunit. Intersubunit communications are strengthened in the presence of external aldimines bound to the beta-site. The motions of the COMM core residues are coordinated with those of the alpha-beta hinge residues beta 174-beta 179 on the interfacial helix beta H6 at the entrance of the hydrophobic tunnel. And the motions of beta H6 are coupled, via helix beta HI and alpha L6, to those of the loop alpha L2 that includes the alpha-subunit catalytically active residue Asp60. Overall, our analysis sheds light on the molecular machinery underlying subunit communication, and identifies the residues playing a key role in the cooperative transmission of conformational motions across the two reaction sites. C1 Bogazici Univ, Dept Chem Engn, TR-80815 Bebek, Istanbul, Turkey. Bogazici Univ, Ctr Polymer Res, TR-80815 Bebek, Istanbul, Turkey. TUBITAK Adv Polymer Mat Res Ctr, TR-80815 Bebek, Istanbul, Turkey. NCI, Mol Struct Sect, Lab Expt & Computat Biol, Div Basic Sci,NIH, Bethesda, MD 20892 USA. RP Jernigan, RL (reprint author), Bogazici Univ, Dept Chem Engn, TR-80815 Bebek, Istanbul, Turkey. RI Jernigan, Robert/A-5421-2012 NR 47 TC 71 Z9 73 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 23 PY 1999 VL 38 IS 12 BP 3478 EP 3490 DI 10.1021/bi982697v PG 13 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 182QY UT WOS:000079510700004 PM 10090734 ER PT J AU Moro, S Hoffmann, C Jacobson, KA AF Moro, S Hoffmann, C Jacobson, KA TI Role of the extracellular loops of G protein-coupled receptors in ligand recognition: A molecular modeling study of the human P2Y(1) receptor SO BIOCHEMISTRY LA English DT Article ID THYROTROPIN-RELEASING-HORMONE; SITE-DIRECTED MUTAGENESIS; ANTAGONIST BINDING; BETA(2)-ADRENERGIC RECEPTOR; OPIOID RECEPTOR; BETA-GAMMA; RESIDUES; IDENTIFICATION; ACTIVATION; AGONIST AB The P2Y(1) receptor is a G protein-coupled receptor (GPCR) and is stimulated by extracellular ADP and ATP. Site-directed mutagenesis of the three extracellular loops (ELs) of the human P2Y(1) receptor indicates the existence of two essential disulfide bridges (Cys124 in EL1 and Cys202 in EL2; Cys42 in the N-terminal segment and Cys296 in EL3) and several specific ionic and H-bonding interactions (involving Glu209 and Arg287). Through molecular modeling and molecular dynamics simulations, an energetically sound conformational hypothesis for the receptor has been calculated that includes transmembrane (TM) domains (using the electron density map of rhodopsin as a template), extracellular loops, and a truncated N-terminal region. ATP may be docked in the receptor, both within the previously defined TM cleft and within two other regions of the receptor, termed meta-binding sites, defined by the extracellular loops. The first meta-binding site is located outside of the TM bundle, between EL2 and EL3, and the second higher energy site is positioned immediately underneath EL2. Binding at both the principal TM binding site and the lower energy meta-binding sites potentially affects the observed ligand potency. In meta-binding site I, the side chain of Glu209 (EL2) is within hydrogen-bonding distance (2.8 Angstrom) of the ribose O3', and Arg287 (EL3) coordinates both alpha- and beta-phosphates of the triphosphate chain, consistent with the insensitivity in potency of the 5'-monophosphate agonist, HT-AMP, to mutation of Arg287 to Lys. Moreover, the selective reduction in potency of 3'NH2-ATP in activating the E209R mutant receptor is consistent with the hypothesis of direct contact between EL2 and nucleotide ligands. Our findings support ATP binding to at least two distinct domains of the P2Y1 receptor, both outside and within the TM core. The two disulfide bridges present in the human P2Y1 receptor play a major role in the structure and stability of the receptor, to constrain the loops within the receptor, specifically stretching the EL2 over the opening of the TM cleft and thus defining the path of access to the binding site. C1 NIDDKD, Mol Recognit Sect, Bioorgan Chem Lab, NIH, Bethesda, MD 20892 USA. RP Jacobson, KA (reprint author), NIDDK, NIH, LBC, Bldg 8A,Room B1A-19, Bethesda, MD 20892 USA. RI Moro, Stefano/A-2979-2012; Jacobson, Kenneth/A-1530-2009 OI Moro, Stefano/0000-0002-7514-3802; Jacobson, Kenneth/0000-0001-8104-1493 FU Intramural NIH HHS [Z01 DK031116-20] NR 48 TC 97 Z9 98 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 23 PY 1999 VL 38 IS 12 BP 3498 EP 3507 DI 10.1021/bi982369v PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 182QY UT WOS:000079510700006 PM 10090736 ER PT J AU Krushkal, J Ferrell, R Mockrin, SC Turner, ST Sing, CF Boerwinkle, E AF Krushkal, J Ferrell, R Mockrin, SC Turner, ST Sing, CF Boerwinkle, E TI Genome-wide linkage analyses of systolic blood pressure using highly discordant siblings SO CIRCULATION LA English DT Article DE hypertension; blood pressure; genetics; genes ID QUANTITATIVE TRAIT LOCI; HYPERTENSION; GUIDELINES; PAIRS AB Background-Elevated blood pressure is a risk factor for cardiovascular, cerebrovascular, and renal diseases. Complex mechanisms of blood pressure regulation pose a challenge to identifying genetic factors that influence interindividual blood pressure variation in the population at large. Methods and Results-We performed a genome-wide linkage analysis of systolic blood pressure in humans using an efficient, highly discordant, full-sibling design. We identified 4 regions of the human genome that show statistical significant linkage to genes that influence interindividual systolic blood pressure variation (2p22.1 to 2p21, 5q33.3 to 5q34, 6q23.1 to 6q24.1, and 15q25.1 to 15q26.1). These regions contain a number of candidate genes that are involved in physiological mechanisms of blood pressure regulation. Conclusions-These results provide both novel information about genome regions in humans that influence interindividual blood pressure Variation and a basis for identifying the contributing genes. Identification of the functional mutations in these genes may uncover novel mechanisms for blood pressure regulation and suggest new therapies and prevention strategies. C1 Univ Texas, Hlth Sci Ctr, Ctr Human Genet, Houston, TX 77225 USA. Univ Texas, Hlth Sci Ctr, Inst Mol Med, Houston, TX 77225 USA. Univ Pittsburgh, Dept Human Genet, Pittsburgh, PA USA. NHLBI, Bethesda, MD 20892 USA. Mayo Clin & Mayo Fdn, Dept Internal Med, Div Hypertens, Rochester, MN 55905 USA. Univ Michigan, Dept Human Genet, Ann Arbor, MI 48109 USA. RP Boerwinkle, E (reprint author), Univ Texas, Hlth Sci Ctr, Ctr Human Genet, POB 20334, Houston, TX 77225 USA. OI Sing, Charles/0000-0001-5872-7956 FU NHLBI NIH HHS [HL-39107, HL-51021, HL-54481] NR 13 TC 177 Z9 182 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 J9 CIRCULATION JI Circulation PD MAR 23 PY 1999 VL 99 IS 11 BP 1407 EP 1410 PG 4 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 176YJ UT WOS:000079179700004 PM 10086961 ER PT J AU Max, MB Gilron, I AF Max, MB Gilron, I TI Sympathetically maintained pain - Has the emperor no clothes? SO NEUROLOGY LA English DT Editorial Material ID ALPHA-ADRENOCEPTORS; PHENTOLAMINE; HYPERALGESIA; BLOCKADE; HUMANS C1 NIDCR, Pain & Neurosensory Mechanisms Branch, NIH, Bethesda, MD USA. RP Max, MB (reprint author), NIH, Pain Res Clin, Bldg 10,Room 3C-405, Bethesda, MD 20892 USA. NR 19 TC 11 Z9 12 U1 0 U2 0 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0028-3878 J9 NEUROLOGY JI Neurology PD MAR 23 PY 1999 VL 52 IS 5 BP 905 EP 907 PG 3 WC Clinical Neurology SC Neurosciences & Neurology GA 182UQ UT WOS:000079516900002 PM 10102403 ER PT J AU Curb, JD Rodriguez, BL Abbott, RD Petrovitch, H Ross, GW Masaki, KH Foley, D Blanchette, PL Harris, T Chen, R White, LR AF Curb, JD Rodriguez, BL Abbott, RD Petrovitch, H Ross, GW Masaki, KH Foley, D Blanchette, PL Harris, T Chen, R White, LR TI Longitudinal association of vascular and Alzheimer's dementias, diabetes, and glucose tolerance SO NEUROLOGY LA English DT Article ID CORONARY HEART-DISEASE; GLYCATION END-PRODUCTS; JAPANESE-AMERICAN MEN; INFORMANT QUESTIONNAIRE; COGNITIVE FUNCTION; STROKE INCIDENCE; ELDERLY IQCODE; MELLITUS; HAWAII; PERFORMANCE AB Objective: To assess the relationship between impaired glucose tolerance and both vascular dementia and AD. Background: Diabetes and abnormalities of glucose metabolism have been associated with stroke and poor cognitive function. In addition, glycoproteins and glycosylation have been postulated to be associated with the development of neuritic plaques characteristic of AD. Methods: a historical prospective cohort study of Japanese-American men (n = 3,774), who were examined at ages 45 to 68 (1965 through 1968) and again at ages 71 to 93 (1991 through 1993). Measurements were obtained by clinical and home examinations: assessment of glucose intolerance (nonfasting 1 hour after glucose load) from 1965 through 1968 and history of diabetes diagnosed by a physician at examinations given from 1965 through 1968 and from 1976 through 1978. At the 1991 through 1993 examinations, the Cognitive Assessment Screening Instrument (CASI)-an instrument designed for use in cross-cultural settings combining features of the Folstein Mini-Mental State Examination, the Modified Mini-Mental State Examination, and the Hasegawa Dementia Screening Scale-was used. Diagnosis and classification of AD and vascular dementia were made by a consensus panel using neuropsychologic assessment data, a neurologist's evaluation, and information from a family informant. Diagnostic and Statistical Manual of Mental Disorders, 3rd ed., revised criteria were used to establish dementia, and subclassification by cause was based on other published criteria. Results: No association between AD and diabetes, present either 25 or 15 years previously, was found after adjustment for age and education in a multiple regression model. A significant association was found between impaired glucose tolerance at baseline and vascular dementia (p < 0.01). Conclusions: These findings confirm expected relationships between impaired glucose tolerance and stroke-related dementia but do not support an association of disordered glucose metabolism with AD. C1 Univ Hawaii, John A Burns Sch Med, Div Geriatr Med, Honolulu, HI 96817 USA. Kuakini Med Ctr, Honolulu Heart Program, Honolulu, HI USA. Univ Virginia, Charlottesville, VA USA. NIA, Bethesda, MD 20892 USA. NIA, Honolulu, HI USA. Dept Vet Affairs, Honolulu, HI USA. RP Curb, JD (reprint author), Univ Hawaii, John A Burns Sch Med, Div Geriatr Med, 347 N Kuakini St, Honolulu, HI 96817 USA. RI Chen, Robert/B-3899-2009 OI Chen, Robert/0000-0002-8371-8629 FU NHLBI NIH HHS [N01-HC-05102]; NIA NIH HHS [N01-AG-4-2149] NR 42 TC 135 Z9 136 U1 1 U2 4 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0028-3878 J9 NEUROLOGY JI Neurology PD MAR 23 PY 1999 VL 52 IS 5 BP 971 EP 975 PG 5 WC Clinical Neurology SC Neurosciences & Neurology GA 182UQ UT WOS:000079516900013 PM 10102414 ER PT J AU Player, MR Torrence, PF AF Player, MR Torrence, PF TI Phosphorothioate oligodeoxyribonucleotides inhibit ribonuclease L thereby disabling a mechanism of interferon action. SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article ID ANTISENSE CHIMERAS; THERAPEUTIC AGENTS; TARGETING RNA; OLIGONUCLEOTIDES; 2-5A; DNA; COAGULATION; CELLS AB Phosphorothioate oligodeoxyribonucleotides were found to be inhibitors of the 2-5A-dependent RNase L. Inhibitory potency depended upon the chain length of the phosphorothioate oligonucleotide and was dependent on the phosphorothioate substitution pattern, but was not substantially base-dependent. (C) 1999 Elsevier Science Ltd. All rights reserved. C1 NIDDKD, Sect Biomed Chem, Med Chem Lab, NIH, Bethesda, MD 20892 USA. RP Torrence, PF (reprint author), NIDDKD, Sect Biomed Chem, Med Chem Lab, NIH, Bldg 8,Rm B2A02, Bethesda, MD 20892 USA. NR 35 TC 2 Z9 2 U1 0 U2 3 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-894X J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PD MAR 22 PY 1999 VL 9 IS 6 BP 891 EP 894 DI 10.1016/S0960-894X(99)00099-2 PG 4 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA 180FY UT WOS:000079376500019 PM 10206556 ER PT J AU Alvarez, ME Gopinath, E Baldwin, P McPhie, P Sefara, NL Green, TK Richardson, HH AF Alvarez, ME Gopinath, E Baldwin, P McPhie, P Sefara, NL Green, TK Richardson, HH TI Solution conformation of human brain natriuretic peptide by circular dichroism and Fourier transform infrared spectrsocopy. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Scios Nova Inc, Mt View, CA 94043 USA. NIH, Bethesda, MD 20892 USA. Ohio Univ, Dept Chem, Athens, OH 45701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 105-ANYL BP U130 EP U130 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148100327 ER PT J AU Brechbiel, MW AF Brechbiel, MW TI The development of chelating agents for alpha-emitter therapy. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Radioimmune & Inorgan Chem Sect, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 161-NUCL BP U49 EP U49 PN 2 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JP UT WOS:000079148200157 ER PT J AU Burke, TR Yao, ZJ Luo, JH Gao, Y Voigt, J King, CR Yang, DJ AF Burke, TR Yao, ZJ Luo, JH Gao, Y Voigt, J King, CR Yang, DJ TI Non phosphate-containing phosphotyrosyl mimetics and their use in signal transduction studies. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, DBS, Med Chem Lab, NIH, Bethesda, MD 20892 USA. Georgetown Univ, Med Ctr, Washington, DC 20007 USA. RI Yao, Zhu-Jun/E-7635-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 149-MEDI BP U1199 EP U1199 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103794 ER PT J AU Casimiro-Garcia, A Cushman, M De Clercq, E Schols, D Pannecouque, C Witvrouw, M Schaeffer, CA Turpin, JA Williamson, K Rice, WG AF Casimiro-Garcia, A Cushman, M De Clercq, E Schols, D Pannecouque, C Witvrouw, M Schaeffer, CA Turpin, JA Williamson, K Rice, WG TI Synthesis and anti-HIV activity of cosalane analogs incorporating nitrogen in the alkenyl linker chain. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Purdue Univ, Dept Med Chem & Mol Pharmacol, Sch Pharm & Pharmacal Sci, W Lafayette, IN 47907 USA. Katholieke Univ Leuven, Rega Inst Med Res, B-3000 Louvain, Belgium. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Lab Antiviral Drug Mech, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 167-MEDI BP U1205 EP U1205 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103812 ER PT J AU Caughey, WS Priola, SA Raymond, LD Horiuchi, M Raines, AE Caughey, B AF Caughey, WS Priola, SA Raymond, LD Horiuchi, M Raines, AE Caughey, B TI Inhibition of abnormal prion protein formation by porphyrins and phthalocyanines. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NIH, Rocky Mt Labs, Hamilton, MT 59840 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 651-INOR BP U1138 EP U1138 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103603 ER PT J AU Cherukuri, MK Russo, A Mitchell, JB AF Cherukuri, MK Russo, A Mitchell, JB TI Nitroxide free radicals: Chemical evidence for catalytic and stoichiometric antioxidant action. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Radiat Biol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 017-TOXI BP U580 EP U580 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148101794 ER PT J AU Erickson, J Bhat, TN Gulnik, SV Gustchina, E Kato, R Suvorov, L Xie, D Yu, B AF Erickson, J Bhat, TN Gulnik, SV Gustchina, E Kato, R Suvorov, L Xie, D Yu, B TI The not-so-great escape: Drug resistance studies with HIV protease. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Struct Biol Program, SAIC Frederick, Frederick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 261-MEDI BP U1232 EP U1232 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103906 ER PT J AU Gao, Y Yao, ZJ Voigt, J Wu, L Zhang, ZY Burke, TR AF Gao, Y Yao, ZJ Voigt, J Wu, L Zhang, ZY Burke, TR TI The design, synthesis and protein-tyrosine phosphatase inhibitory profiles of non phosphate-containing pTyr mimetics. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, DBS, Med Chem Lab, NIH, Bethesda, MD 20892 USA. Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA. RI Yao, Zhu-Jun/E-7635-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 175-MEDI BP U1207 EP U1207 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103820 ER PT J AU Gupta, PK Sun, H Biumbergs, P Snader, KM Donohue, S AF Gupta, PK Sun, H Biumbergs, P Snader, KM Donohue, S TI Synthesis and toxicity studies of 2-Fluoro-8-chloro-cyclic AMP SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Ash Stevens Inc, Detroit, MI 48202 USA. NCI, Pharmaceut Resources Branch, Bethesda, MD 20892 USA. NCI, Toxicol & Pharmacol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 188-MEDI BP U1211 EP U1211 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103833 ER PT J AU Hernandez, LM Nieves-Martinez, I Mason, RP AF Hernandez, LM Nieves-Martinez, I Mason, RP TI Determination of vanadate-mediated NAD(P)H oxidation mechanism under aerobic and anaerobic conditions. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Puerto Rico, CUH Stn, Humacao, PR 00791 USA. NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 322-CHED BP U399 EP U399 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148101166 ER PT J AU Jayaraman, M Cushman, M Pommier, Y Strumberg, D AF Jayaraman, M Cushman, M Pommier, Y Strumberg, D TI Synthesis of new analogs of indenoisoquinoline: Potential non-camptothecin topoisomerase I poisons. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Purdue Univ, Sch Pharm, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA. NCI, Div Basic Sci, Mol Pharmacol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 018-MEDI BP U1160 EP U1160 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103664 ER PT J AU Keefer, LK Srinivasan, A Saavedra, JE Hrabie, JA Toscano, JP Davies, KM AF Keefer, LK Srinivasan, A Saavedra, JE Hrabie, JA Toscano, JP Davies, KM TI Diazeniumdiolates: Interrelationships with more traditional N-nitroso compounds. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Comparat Carcinogenesis Lab, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC Frederick, Frederick, MD 21702 USA. Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA. George Mason Univ, Dept Chem, Fairfax, VA 22030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 003-TOXI BP U576 EP U576 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148101780 ER PT J AU Lin, ZW Neamati, N Pommier, Y Burke, TR AF Lin, ZW Neamati, N Pommier, Y Burke, TR TI Solid-phase synthesis of potential HIV-1 integrase inhibitors. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, DBS, Med Chem Lab, NIH, Bethesda, MD 20892 USA. NCI, DBS, Mol Pharmacol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 174-MEDI BP U1207 EP U1207 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103819 ER PT J AU Luzzio, FA Zacherl, DP Figg, WD AF Luzzio, FA Zacherl, DP Figg, WD TI A facile scheme for phthalimide reversible arrow phthalimidine conversion SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Louisville, Dept Chem, Louisville, KY 40292 USA. NCI, Med Branch, Bethesda, MD 20892 USA. RI Figg Sr, William/M-2411-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 422-ORGN BP U191 EP U192 PN 2 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 176JP UT WOS:000079148200622 ER PT J AU Mitchell, JB Russo, A Cherukuri, MK AF Mitchell, JB Russo, A Cherukuri, MK TI A survey of in vivo antioxidant effects of nitroxides. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Radiat Biol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 018-TOXI BP U581 EP U581 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148101795 ER PT J AU Schieck, CL Lee, KS Jones, DW Gorey, JG Saunders, RC Urbina, RA Knable, MB Weinberger, DR Glennon, RA AF Schieck, CL Lee, KS Jones, DW Gorey, JG Saunders, RC Urbina, RA Knable, MB Weinberger, DR Glennon, RA TI [123I]-DOI: A novel 5-HT2 receptor SPECT imaging agent. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Virginia Commonwealth Univ, Richmond, VA 23298 USA. NIMH, Washington, DC 20032 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 247-MEDI BP U1228 EP U1228 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103892 ER PT J AU Shanklin, AP Saavedra, JE Hrabie, JA Citro, ML Keefer, LK AF Shanklin, AP Saavedra, JE Hrabie, JA Citro, ML Keefer, LK TI Piperazine as a linker for binding the nitric oxide-releasing diazeniumdiolate function to other biomedically important molecules. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Frederick, MD 21702 USA. SAIC Frederick, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 208-MEDI BP U1216 EP U1217 PN 1 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103853 ER PT J AU Shim, JY Welsh, WJ Berglund, BA Howlett, AC Fleming, PF Rice, KC AF Shim, JY Welsh, WJ Berglund, BA Howlett, AC Fleming, PF Rice, KC TI Prediction of the bioactive conformation of arachidonyl ethanolamide using distance constrained systematic search. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 Univ Missouri, Dept Chem, St Louis, MO 63121 USA. St Louis Univ, Sch Med, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 030-COMP BP U650 EP U650 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148102006 ER PT J AU Waugh, DS Kapust, RB Fox, JD AF Waugh, DS Kapust, RB Fox, JD TI E-coli maltose binding protein is uncommonly effective at inhibiting the aggregation of proteins to which it is fused SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Prot Engn Grp, Macromol Struct Lab,ABL Basic Res Program, Frederick, MD 21702 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 092-BIOT BP U181 EP U181 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148100474 ER PT J AU Yao, ZJ Luo, JH Gao, Y Yang, DJ Voigt, J King, CR Burke, TR AF Yao, ZJ Luo, JH Gao, Y Yang, DJ Voigt, J King, CR Burke, TR TI Synthesis and evaluation of high affinity non-phosphate containing GrB2 SH2 domain ligands. SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract C1 NCI, DBS, Med Chem Lab, NIH, Bethesda, MD 20892 USA. Georgetown Univ, Med Ctr, Washington, DC 20007 USA. RI Yao, Zhu-Jun/E-7635-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD MAR 21 PY 1999 VL 217 MA 176-MEDI BP U1207 EP U1207 PN 1 PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA 176JN UT WOS:000079148103821 ER PT J AU Clay, JR Shrier, A AF Clay, JR Shrier, A TI On the role of subthreshold dynamics in neuronal signaling SO JOURNAL OF THEORETICAL BIOLOGY LA English DT Article ID STOCHASTIC RESONANCE; MODEL; BIFURCATION; INFORMATION; SYSTEMS; INVITRO; CHAOS AB The role of subthreshold dynamics in neuronal signaling is examined using periodic pulse train stimulation of the Fitzhugh-Nagumo (FN) model of nerve membrane excitability and results from the squid giant axon as an experimental data base. For a broad range of stimulus conditions the first pulse in a pulse train elicited an action potential, whereas all subsequent pulses elicited subthreshold responses, both in the axon and in the FN model. These results are not well described by the Hodgkin and Huxley 1952 model. Various different patterns of subthreshold responses, including chaotic dynamics, can be observed in both systems-the FN model and the axon-depending upon stimulus conditions. For some conditions action potentials are occasionally interspersed among the subthreshold events with randomly occurring interspike intervals. The randomness is directly attributable to the underlying subthreshold chaos-deterministic chaos-rather then to a stochastic noise source. We conclude that this mechanism may contribute to multimodal interspike interval histograms which have been observed from individual neurons throughout the nervous system. (C) 1999 Academic Press. C1 NINDS, Neurophysiol Lab, NIH, Bethesda, MD 20892 USA. McGill Univ, Dept Physiol, Montreal, PQ H3G 1Y6, Canada. RP Clay, JR (reprint author), NINDS, Neurophysiol Lab, NIH, Bethesda, MD 20892 USA. NR 25 TC 14 Z9 14 U1 0 U2 2 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-5193 J9 J THEOR BIOL JI J. Theor. Biol. PD MAR 21 PY 1999 VL 197 IS 2 BP 207 EP 216 DI 10.1006/jtbi.1998.0867 PG 10 WC Biology; Mathematical & Computational Biology SC Life Sciences & Biomedicine - Other Topics; Mathematical & Computational Biology GA 176ZN UT WOS:000079182500006 PM 10074394 ER PT J AU Leno, M Carter, L Venzon, DJ Romano, J Markham, PD Limbach, K Tartaglia, J Paoletti, E Benson, J Franchini, G Robert-Guroff, M AF Leno, M Carter, L Venzon, DJ Romano, J Markham, PD Limbach, K Tartaglia, J Paoletti, E Benson, J Franchini, G Robert-Guroff, M TI CD8(+) lymphocyte antiviral activity in monkeys immunized with SIV recombinant poxvirus vaccines: Potential role in vaccine efficacy SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID SIMIAN-IMMUNODEFICIENCY-VIRUS; CELL-MEDIATED SUPPRESSION; RHESUS MACAQUES; HIV-1 INFECTION; T-CELLS; PROTECTION; REPLICATION; INTERLEUKIN-12; RESPONSES; ENVELOPE AB Protection against intravenous sinian immunodeficiency virus (SIV) challenge was assessed in rhesus macaques after immunization with a highly attenuated vaccinia (NYVAC)-SIV recombinant. One-third of vaccinated animals controlled viral infection and progressed to disease more slowly than control animals (Benson J, et al.: J Virol 1998;72:4170). However, this protection was not associated with neutralizing antibodies, cytotoxic T lymphocytes, or helper T cell responses. To explore other potential correlates of protection, me examined CD8(+) T cell antiviral activity in macaques vaccinated with NYVAC-SIV, with or without added cytokine adjuvants, and in controls receiving only IL-12 or IL-12 plus IL-2. Before immunization, naive macaques exhibited a broad range of CD8(+) T cell antiviral activity. Nevertheless, in the course of immunization, the vaccinated macaques as a group developed increased CD8(+) T cell antiviral activity while the controls remained stable. Infectious SIV exposure also increased antiviral activity. Prechallenge antiviral activity levels of vaccinated macaques were not sufficient to prevent SN transmission or control viral replication during acute infection. However, vaccinated animals consistently exhibited reduced viral loads postchallenge compared with controls. Moreover, high suppressive activity 8 weeks postchallenge, at which time the viremia set point was established, was significantly correlated with reduced viral load and slow disease progression. Prechallenge antiviral activity influenced this result, as decreased viremia and slow progressor status were more apparent in macaques with high suppressive activity both pre- and postchallenge. Our data demonstrate the impact of CD8(+) antiviral activity on viral replication and disease progression, and suggest that vaccine designs able to elicit high levels of this activity will contribute significantly to protective efficacy. C1 NCI, Basic Res Lab, Bethesda, MD 20892 USA. NCI, Biostat & Data Management Sect, Bethesda, MD 20892 USA. Adv Biosci Labs Inc, Kensington, MD 20895 USA. Virogenet Corp, Troy, NY 12180 USA. RP Robert-Guroff, M (reprint author), NCI, Basic Res Lab, Bldg 37,Room 6A11,37 Convent Dr,MSC 4255, Bethesda, MD 20892 USA. EM guroffin@dc37a.nci.nih.gov NR 52 TC 27 Z9 27 U1 0 U2 1 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 0889-2229 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD MAR 20 PY 1999 VL 15 IS 5 BP 461 EP 470 DI 10.1089/088922299311213 PG 10 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA 178MH UT WOS:000079269400008 PM 10195756 ER PT J AU Hohmann, AG Briley, EM Herkenham, M AF Hohmann, AG Briley, EM Herkenham, M TI Pre- and postsynaptic distribution of cannabinoid and mu opioid receptors in rat spinal cord SO BRAIN RESEARCH LA English DT Article DE dorsal rhizotomy; autoradiography; deafferentation; superficial laminae; anandamide ID SUPERFICIAL DORSAL HORN; BINDING-SITES; AUTORADIOGRAPHIC LOCALIZATION; ROOT GANGLIA; RHIZOTOMY; DELTA; NEURONS; OPIATE; BRAIN; IMMUNOREACTIVITY AB In vitro receptor binding and quantitative autoradiography were used to assess the pre- and postsynaptic distribution of cannabinoid receptors in the cervical dorsal horn of the rat spinal cord. An extensive unilateral dorsal rhizotomy was performed across seven or eight successive spinal segments from C3 to T1 or T2. The densities of cannabinoid and mu opioid receptors in the central (C6) spinal segment were assessed 2, 4, 8, and 16 days post rhizotomy and compared with those of untreated rats. Rhizotomy induced approximately a 50% ipsilateral loss in the [H-3]CP55,940 binding to spinal cannabinoid receptors that was maximal at 8 days post-rhizotomy. By comparison, the binding of [H-3][D-Ala(2)-MePhe(4),Gly-ol(5)]enkephalin (DAMGO) to mu receptors was depleted approximately 60% in near-adjacent sections. By contrast, changes in [H-3]CP55,940 binding contralateral to the deafferentation were largely absent at all post-lesion delays. These data suggest that under conditions in which a spinal segment is completely deafferented, approximately 50% of cannabinoid receptors in the cervical (C6) dorsal horn reside presynaptically on central terminals of primary afferents. The present data provide anatomical evidence for presynaptic as well as postsynaptic localization of cannabinoid receptors in the spinal dorsal hem. (C) 1999 Published by Elsevier Science B.V. C1 NIMH, Funct Neuroanat Sect, Bethesda, MD 20892 USA. RP Hohmann, AG (reprint author), Natl Inst Dent & Craniofacial Res, Cellular Neurosci Sect, Pain & Neurosensory Mechanisms Branch, Bldg 49,Room 1A11, Bethesda, MD 20892 USA. OI Herkenham, Miles/0000-0003-2228-4238 NR 41 TC 115 Z9 116 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 20 PY 1999 VL 822 IS 1-2 BP 17 EP 25 DI 10.1016/S0006-8993(98)01321-3 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 178HW UT WOS:000079261100003 PM 10082879 ER PT J AU Blackwell, KT Alkon, DL AF Blackwell, KT Alkon, DL TI Ryanodine receptor modulation of in vitro associative learning in Hermissenda crassicornis SO BRAIN RESEARCH LA English DT Article DE classical conditioning; calcium-induced calcium release; ryanodine receptor; photoreceptor; associative learning; light adaptation ID PROTEIN-KINASE-C; LONG-TERM POTENTIATION; CEREBELLAR PURKINJE-CELLS; METABOTROPIC GLUTAMATE RECEPTORS; PHOSPHOLIPASE-C; CA2+ RELEASE; INOSITOL TRISPHOSPHATE; SARCOPLASMIC-RETICULUM; VENTRAL PHOTORECEPTORS; SYNAPTIC FACILITATION AB Classical conditioning of the mollusc, Hermissenda crassicornis, is a model system used to study cellular correlates of associative learning. Paired presentation of light and turbulence, but not unpaired presentations, causes Hermissenda to contract its foot in response to light alone. Intracellular recordings from the type B photoreceptors of the Hermissenda eye reveal a learning specific increase of input resistance, and a reduction of voltage-dependent potassium currents, both of which depend on an elevation of intracellular calcium. Two previously demonstrated sources of calcium are influx through voltage-dependent channels, and release of calcium from intracellular stores through the IP3 receptor channel. Both modeling studies and identification of memory-related genes using RNA fingerprinting suggest that a third source of calcium, release from intracellular stores through the ryanodine receptor, may be involved in classical conditioning. We describe here an experiment suggesting that this third source of calcium is necessary for the cellular changes underlying associative memory storage. Paired presentations of a light stimulus with a turbulence stimulus resulted in a significant increase in input resistance. Unpaired presentations of light and turbulence did not produce a significant increase in input resistance. A third group of nervous systems first was incubated in dantrolene to block release of calcium through the ryanodine receptor, and then received paired training. There was no change in input resistance for this group. The effect of dantrolene on light adaptation of the photoreceptor was assessed by measuring the generator potential of a second light pulse presented some number of seconds after a first light pulse. The results show that at interpulse intervals of 5 s, 10 s and 20 s, the generator potential of the dantrolene group is significantly greater than that of the control group. These results suggest a role for the ryanodine receptor in both a cellular correlate of classical conditioning and light adaptation. (C) 1999 Elsevier Science B.V. All rights reserved. C1 George Mason Univ, Krasnow Inst, Inst Computat Sci & Informat, Fairfax, VA 22030 USA. NINDS, Lab Adapt Syst, NIH, Bethesda, MD 20892 USA. RP Blackwell, KT (reprint author), George Mason Univ, Krasnow Inst, Inst Computat Sci & Informat, MS 2A1, Fairfax, VA 22030 USA. EM avrama@gmu.edu OI Blackwell, Kim/0000-0003-4711-2344 FU NIMH NIH HHS [K21-MH01141, K21 MH001141] NR 60 TC 27 Z9 28 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 20 PY 1999 VL 822 IS 1-2 BP 114 EP 125 DI 10.1016/S0006-8993(99)01105-1 PG 12 WC Neurosciences SC Neurosciences & Neurology GA 178HW UT WOS:000079261100013 PM 10082889 ER PT J AU Seth, P AF Seth, P TI Cancer gene therapy using adenoviral vectors expressing tumor suppressor genes SO HUMAN GENE THERAPY LA English DT Meeting Abstract C1 NCI, Med Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD MAR 20 PY 1999 VL 10 IS 5 BP 842 EP 842 PG 1 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA 181EY UT WOS:000079429200030 ER PT J AU Li, HW Alonso-Vanegas, M Colicos, MA Jung, SS Lochmuller, H Sadikot, AF Seth, P Snipes, GJ Karpati, G Nalbantoglu, J AF Li, HW Alonso-Vanegas, M Colicos, MA Jung, SS Lochmuller, H Sadikot, AF Seth, P Snipes, GJ Karpati, G Nalbantoglu, J TI Rapid elimination of transplanted intracerebral malignant glioma by adenovirus mediated p53 gene overexpression. SO HUMAN GENE THERAPY LA English DT Meeting Abstract C1 McGill Univ, Montreal Neurol Inst, Dept Neurol & Neurosurg, Montreal, PQ H3A 2B4, Canada. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU MARY ANN LIEBERT INC PUBL PI LARCHMONT PA 2 MADISON AVENUE, LARCHMONT, NY 10538 USA SN 1043-0342 J9 HUM GENE THER JI Hum. Gene Ther. PD MAR 20 PY 1999 VL 10 IS 5 BP 849 EP 849 PG 1 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental SC Biotechnology & Applied Microbiology; Genetics & Heredity; Research & Experimental Medicine GA 181EY UT WOS:000079429200047 ER PT J AU Feng, LY Wang, CY Jiang, H Oho, C Mizuno, K Dugich-Djordjevic, M Lu, B AF Feng, LY Wang, CY Jiang, H Oho, C Mizuno, K Dugich-Djordjevic, M Lu, B TI Differential effects of GDNF and BDNF on cultured ventral mesencephalic neurons SO MOLECULAR BRAIN RESEARCH LA English DT Article DE GDNF; BDNF; mesencephalic; transmitter release; synaptic protein; fasciculation ID MIDBRAIN DOPAMINERGIC-NEURONS; RECEPTOR TYROSINE KINASE; RAT SUBSTANTIA-NIGRA; NEUROTROPHIC FACTOR; IN-VIVO; PARKINSONS-DISEASE; NERVOUS-SYSTEM; GROWTH-FACTOR; BRAIN; RET AB Previous studies have shown that brain derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) can enhance the survival of dopaminergic neurons in the ventral mesencephalon (VM). Here we compared several non-survival functions of the two factors in VM neurons in culture. We found that both BDNF and GDNF elicited an increase in the depolarization-induced release of dopamine, hut had no effect on GABA release, in the VM cultures. BDNF, but not GDNF, significantly enhanced the expression of the calcium binding protein calbindin and synaptic protein SNAP25, In contrast, treatment of the cultures with GDNF, but not BDNF, elicited a marked fasciculation of the processes of the VM neurons. Thus, although both act on VM neurons, BDNF and GDNF have distinct functions. (C) 1999 Published by Elsevier Science B.V. All rights reserved. C1 NICHD, Dev Neurobiol Lab, Unit Synapse Dev & Plast, NIH, Bethesda, MD 20892 USA. NICHD, Growth Factors Sect, Bethesda, MD 20892 USA. Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai 200031, Peoples R China. Chinese Acad Sci, Shanghai Brain Res Inst, Shanghai 200031, Peoples R China. George Washington Univ, Grad Program Genet, Washington, DC 20052 USA. RP Lu, B (reprint author), NICHD, Dev Neurobiol Lab, Unit Synapse Dev & Plast, NIH, Bldg 49,Rm 5A38,49 Convent Dr,MSC4480, Bethesda, MD 20892 USA. EM lub@codon.nih.gov NR 65 TC 33 Z9 34 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-328X J9 MOL BRAIN RES JI Mol. Brain Res. PD MAR 20 PY 1999 VL 66 IS 1-2 BP 62 EP 70 DI 10.1016/S0169-328X(99)00015-7 PG 9 WC Neurosciences SC Neurosciences & Neurology GA 180MF UT WOS:000079389200007 ER PT J AU Ho, N Roig, C Diadori, P AF Ho, N Roig, C Diadori, P TI Epidermal nevi and localized cranial defects SO AMERICAN JOURNAL OF MEDICAL GENETICS LA English DT Article DE epidermal nevi; giant hairy pigmented nevi; localized cranial defects AB We report on a girl with a congenital pigmented hairy nevus of the scalp, epidermal nevi of the right temple, and localized cranial defects. We have not found other reported cases of giant pigmented hairy nevus of the scalp occurring with absence of underlying cranial bone. We speculate that the localized cranial defects are undergrowth anomalies representative of a paracrinopathy from the overlying nevus or simultaneous bone/skin dysplasia, the former having been resorbed, In the absence of a familial history of epidermal nevi and/or seizures, our patient represents a sporadic case, perhaps a somatic mutation. (C) 1999 Wiley-Liss, Inc. C1 NHGRI, Med Genet Branch, NIH, Bethesda, MD 20892 USA. Alberta Childrens Hosp, Dept Neurol, Calgary, AB, Canada. RP Ho, N (reprint author), NHGRI, Med Genet Branch, NIH, Bethesda, MD 20892 USA. NR 14 TC 5 Z9 5 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0148-7299 J9 AM J MED GENET JI Am. J. Med. Genet. PD MAR 19 PY 1999 VL 83 IS 3 BP 187 EP 190 DI 10.1002/(SICI)1096-8628(19990319)83:3<187::AID-AJMG8>3.3.CO;2-N PG 4 WC Genetics & Heredity SC Genetics & Heredity GA 171TF UT WOS:000078879600008 PM 10096594 ER PT J AU Hunt, CR Parsian, AJ Goswami, PC Kozak, CA AF Hunt, CR Parsian, AJ Goswami, PC Kozak, CA TI Characterization and expression of the mouse Hsc70 gene SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION LA English DT Article DE Hsc70 DNA sequence; azetidine; MG132; cell cycle; gene mapping; gene expression ID SHOCK COGNATE PROTEIN; DNA-BINDING ACTIVITY; HEAT-SHOCK; CELL-CYCLE; CLONING; TRANSCRIPTION; LOCALIZATION; ACTIVATION; RECEPTOR; STRESS AB A genomic clone encoding the mouse Hsc70 gene has been isolated and characterized by DNA sequence analysis. The gene is approximately 3.9 kb in length and contains eight introns, the fifth, sixth and eighth of which encode the three U14 snoRNAs. The gene has been located on Chr 9 in the order Fli1-Itm1-Olfr7-Hsc70(Rnu14)-Cbl by genetic analysis. Expression of Hsc70 is universal in all tissues of the mouse, but is slightly elevated in liver, skeletal muscle and kidney tissue, while being depressed in testes. In cultured mouse NIH 3T3 cells or human HeLa cells, Hsc70 mRNA levels are low under normal conditions, but can be induced 8-fold higher in both lines by treatment with the amino acid analog azetidine. A similar induction is seen in cells treated with the proteosome inhibitor MG132 suggesting that elevated Hsc70 expression may be coupled to protein degradation. Surprisingly, expression of the human Hsc70 gene is also regulated by cell-cycle position being 8-10-fold higher in late G(1)/S-phase cells as opposed to the levels in early G(1)-phase cells. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Washington Univ, Sch Med, Radiat Oncol Ctr, St Louis, MO 63108 USA. NIAID, Bethesda, MD 20892 USA. RP Hunt, CR (reprint author), Washington Univ, Sch Med, Radiat Oncol Ctr, 4511 Forest Pk Blvd, St Louis, MO 63108 USA. EM hunt@radonc.wustl.edu FU NCI NIH HHS [R01 CA60757, R29 CA/GM69593] NR 37 TC 18 Z9 21 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4781 J9 BBA-GENE STRUCT EXPR JI Biochim. Biophys. Acta-Gene Struct. Expression PD MAR 19 PY 1999 VL 1444 IS 3 BP 315 EP 325 DI 10.1016/S0167-4781(98)00285-1 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 179XM UT WOS:000079355400001 PM 10095055 ER PT J AU Czirjak, G Burkhart, WA Moyer, MB Antal, J Shears, SB Enyedi, P AF Czirjak, G Burkhart, WA Moyer, MB Antal, J Shears, SB Enyedi, P TI Cloning and functional expression of the cytoplasmic form of rat aminopeptidase P SO BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION LA English DT Article DE aminopeptidase P; cDNA cloning; functional expression; cytoplasmic ID X-PROLYL-AMINOPEPTIDASE; BOVINE LUNG; PIG-KIDNEY; ESCHERICHIA-COLI; PURIFICATION; ENZYME; DEGRADATION; BRADYKININ; CLEAVAGE; PEPTIDES AB A rat cytoplasmic aminopeptidase P was purified from liver cytosol with a procedure including an affinity elution step with 3 mu M inositol 1,3,4-trisphosphate. Proteolytic fragments were generated, sequenced and the enzyme was cloned from a rat liver cDNA library. The structure shows high (87.8% and 95.5%, respectively) sequence identity at the nucleotide and amino acid levels with the previously described human putative cytoplasmic aminopeptidase P. The cloned rat enzyme was functionally expressed in Escherichia coli and also in COS-1 cells. Western blot analysis, using an antibody generated against the recombinant protein, and Northern blot hybridization showed ubiquitous expression of the protein in different tissues with the highest expression level in the testis. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Semmelweis Univ Med, Dept Physiol, H-1444 Budapest, Hungary. Glaxo Res Inst, Res Triangle Pk, NC 27709 USA. Agr Biotechnol Ctr, ACE Lab, H-2101 Godollo, Hungary. NIEHS, Inositol Lipid Sect, Lab Signal Transduct, NIH, Res Triangle Pk, NC 27709 USA. RP Enyedi, P (reprint author), Semmelweis Univ Med, Dept Physiol, POB 259, H-1444 Budapest, Hungary. NR 31 TC 14 Z9 15 U1 1 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4781 J9 BBA-GENE STRUCT EXPR JI Biochim. Biophys. Acta-Gene Struct. Expression PD MAR 19 PY 1999 VL 1444 IS 3 BP 326 EP 336 DI 10.1016/S0167-4781(99)00005-6 PG 11 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 179XM UT WOS:000079355400002 PM 10095056 ER PT J AU Wibbenmeyer, JA Xavier, KA Smith-Gill, SJ Willson, RC AF Wibbenmeyer, JA Xavier, KA Smith-Gill, SJ Willson, RC TI Cloning, expression, and characterization of the Fab fragment of the anti-lysozyme antibody HyHEL-5 SO BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY LA English DT Article DE antibody; calorimetry; Fab; lysozyme; expression ID SITE-DIRECTED MUTAGENESIS; EGG-WHITE LYSOZYME; SINGLE-CHAIN FV; MONOCLONAL-ANTIBODY; BINDING; COMPLEX; ASSOCIATION; PROTEINS AB Hybridoma cDNAs encoding the individual chains of the Fab fragment of the well characterized murine monoclonal antibody HyHEL-5 were cloned and sequenced. The recombinant Fab fragment was produced by expressing each chain in a separate Escherichia coli pET vector, denaturing inclusion bodies and co-refolding. Characterization of the purified Fab by MALDI-TOF mass spectrometry and N-terminal amino acid sequencing demonstrated proper processing of the individual chains. The association of the recombinant Fab fragment with hen egg lysozyme and the avian epitope variant bobwhite quail lysozyme was found by isothermal titration calorimetry to have energetics very similar to that of the HyHEL-5 IgG. Heterologous expression of the HyHEL-5 Fab fragment opens the way to structure/function studies in this well-known system. (C) 1999 Published by Elsevier Science B.V. All rights reserved. C1 Univ Houston, Dept Biol & Biochem, Houston, TX 77024 USA. Univ Houston, Dept Chem Engn, Houston, TX 77024 USA. NCI, Genet Lab, NIH, Bethesda, MD 20892 USA. RP Willson, RC (reprint author), Univ Houston, Dept Biol & Biochem, 4800 Calhoun Ave, Houston, TX 77024 USA. EM willson@uh.edu NR 29 TC 8 Z9 11 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-4838 J9 BBA-PROTEIN STRUCT M JI Biochim. Biophys. Acta-Protein Struct. Molec. Enzym. PD MAR 19 PY 1999 VL 1430 IS 2 BP 191 EP 202 DI 10.1016/S0167-4838(98)00285-4 PG 12 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 180XT UT WOS:000079411700003 PM 10082947 ER PT J AU Hurley, JH AF Hurley, JH TI Structure, mechanism, and regulation of mammalian adenylyl cyclase SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Review ID CALMODULIN-BINDING DOMAIN; IN-VIVO; NUCLEOSIDE 3'-POLYPHOSPHATES; CATALYTIC MECHANISM; CYTOSOLIC DOMAINS; PROTEIN-KINASE; GS-ALPHA; INHIBITION; PHOSPHORYLATION; IDENTIFICATION C1 NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. RP Hurley, JH (reprint author), NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. NR 54 TC 165 Z9 165 U1 4 U2 15 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 19 PY 1999 VL 274 IS 12 BP 7599 EP 7602 DI 10.1074/jbc.274.12.7599 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178LU UT WOS:000079268100001 PM 10075642 ER PT J AU Ng, GYK Clark, J Coulombe, N Ethier, N Hebert, TE Sullivan, R Kargman, S Chateauneuf, A Tsukamoto, N McDonald, T Whiting, P Mezey, E Johnson, MP Liu, QY Kolakowski, LF Evans, JF Bonner, TI O'Neill, GP AF Ng, GYK Clark, J Coulombe, N Ethier, N Hebert, TE Sullivan, R Kargman, S Chateauneuf, A Tsukamoto, N McDonald, T Whiting, P Mezey, E Johnson, MP Liu, QY Kolakowski, LF Evans, JF Bonner, TI O'Neill, GP TI Identification of a GABA(B) receptor subunit, gb2, required for functional GABA(B) receptor activity SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID EXPRESSION CLONING; NERVOUS-SYSTEM; RAT-BRAIN; PEPTIDES AB G protein-coupled receptors are commonly thought to bind their cognate ligands and elicit functional responses primarily as monomeric receptors. In studying the recombinant gamma-aminobutyric acid, type B (GABA,) receptor (gb1a) and a GABA(B)-like orphan receptor (gb2), we observed that both receptors are functionally inactive when expressed individually in multiple heterologous systems. Characterization of the tissue distribution of each of the receptors by in situ hybridization histochemistry in rat brain revealed co-localization of gb1 and gb2 transcripts in many brain regions, suggesting the hypothesis that gb1 and gb2 may interact in vivo. In three established functional systems (inwardly rectifying K+ channel currents in Xenopus oocytes, melanophore pigment aggregation, and direct cAMP measurements in HEK-293 cells), GABA mediated a functional response in cells coexpressing gb1a and gb2 brit not in cells expressing either receptor individually. This GABA activity could be blocked with the GABA(B) receptor antagonist CGP71872, In COS-7 cells coexpressing gb1a and gb2 receptors, co-immunoprecipitation of gb1a and gb2 receptors was demonstrated, indicating that gb1a and gb2 act as subunits in the formation of a functional GABA(B) receptor. C1 Merck Frosst Ctr Therapeut Res, Kirkland, PQ H9H 3L1, Canada. Merck Sharp & Dohme Ltd, Res Labs, Harlow CM20 2QR, Essex, England. Banyu Pharmaceut Co Ltd, Tsukuba, Ibaraki 3002611, Japan. Montreal Heart Inst, Montreal, PQ H1T 1C8, Canada. NINDS, NIH, Bethesda, MD 20892 USA. NIMH, Genet Sect, Bethesda, MD 20892 USA. Univ Texas, Hlth Sci Ctr, Dept Pharmacol, San Antonio, TX 78284 USA. Univ Texas, Hlth Sci Ctr, Dept Biochem, San Antonio, TX 78284 USA. RP Ng, GYK (reprint author), Merck Frosst Ctr Therapeut Res, Kirkland, PQ H9H 3L1, Canada. EM gordon_ng@merck.com NR 22 TC 165 Z9 173 U1 0 U2 0 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 19 PY 1999 VL 274 IS 12 BP 7607 EP 7610 DI 10.1074/jbc.274.12.7607 PG 4 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178LU UT WOS:000079268100003 PM 10075644 ER PT J AU Lee, AC Fenster, BE Ito, H Takeda, K Bae, NS Hirai, T Yu, ZX Ferrans, VJ Howard, BH Finkel, T AF Lee, AC Fenster, BE Ito, H Takeda, K Bae, NS Hirai, T Yu, ZX Ferrans, VJ Howard, BH Finkel, T TI Ras proteins induce senescence by altering the intracellular levels of reactive oxygen species SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID HUMAN-FIBROBLASTS; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; GROWTH ARREST; IN-VIVO; CELLS; GENERATION; TRANSFORMATION; INVOLVEMENT; APOPTOSIS AB Human diploid fibroblasts eventually lose the capacity to replicate in culture and enter a viable but nonproliferative state of senescence. Recently, it has been demonstrated that retroviral-mediated gene transfer into primary fibroblasts of an activated ras gene (V12ras) rapidly accelerates development of the senescent phenotype, Using this in vitro system, we have sought to define the mediators of Res-induced senescence. We demonstrate that expression of V12Ras results in an increase in intracellular and in particular, mitochondrial reactive oxygen species, The ability of V12Ras to induce growth arrest and senescence is shown to be partially inhibited by coexpression of an activated rad gene. A more dramatic rescue of V12Ras-expressing cells is demonstrated when the cells are placed in a low oxygen environment, a condition in which reactive oxygen species production is inhibited. In addition, in a 1% oxygen environment, Res is unable to trigger an increase in the level of the cyclin-dependent kinase inhibitor p21 or to activate the senescent program. Under normoxic (20% O-2) conditions, the V12Ras senescent phenotype is demonstrated to be unaffected by scavengers of superoxide but rescued by scavengers of hydrogen peroxide. These results suggest that in normal diploid cells, Ras proteins regulate oxidant production and that a rise in intracellular H2O2 represents a critical signal mediating replicative senescence. C1 NHLBI, Cardiol Branch, NIH, Bethesda, MD 20892 USA. NHLBI, Pathol Sect, NIH, Bethesda, MD 20892 USA. NICHD, Lab Mol Growth Regulat, NIH, Bethesda, MD 20892 USA. RP Finkel, T (reprint author), NHLBI, Cardiol Branch, NIH, 10 Ctr Dr, Bethesda, MD 20892 USA. EM finkelt@gwgate.nhlbi.nih.gov NR 44 TC 399 Z9 407 U1 0 U2 17 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 19 PY 1999 VL 274 IS 12 BP 7936 EP 7940 DI 10.1074/jbc.274.12.7936 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178LU UT WOS:000079268100048 PM 10075689 ER PT J AU Iwata, T Sato, S Jimenez, J McGowan, M Moroni, M Dey, A Ibaraki, N Reddy, VN Carper, D AF Iwata, T Sato, S Jimenez, J McGowan, M Moroni, M Dey, A Ibaraki, N Reddy, VN Carper, D TI Osmotic response element is required for the induction of aldose reductase by tumor necrosis factor-alpha SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID INDUCED INSULIN-RESISTANCE; TYROSINE PHOSPHORYLATION; PROTEASOME ACTIVITIES; RECEPTOR SUBSTRATE-1; KAPPA-B; GENE; EXPRESSION; INHIBITION; PROTEIN; CELLS AB Induction of aldose reductase (AR) was observed in human cells treated with tumor necrosis factor-alpha (TNF-alpha). AR protein expression increased severalfold in human liver cells after 1 day of exposure to 100 units/ml TNF-a. An increase in AR transcripts was also observed in human liver cells after 3 h of TNF-alpha treatment, reaching a maximum level of 11-fold at 48 h, Among the three inflammatory cytokines: TNF-alpha, interleukin-1, and interferon-gamma, TNF-alpha (100 units/ml) gave the most induction of AR. Differences in the pattern of AR induction were observed in human liver, lens, and retinal pigment epithelial cells with increasing concentrations of TNF-alpha. A similar pattern of AR promoter response was observed between TNF-alpha and osmotically stressed human liver cells. The deletion of the osmotic response element (ORE) abolished the induction by TNF-alpha and osmotic stress. A point mutation that converts ORE to a nuclear factor-kappa B (NF-kappa B) sequence abolished the osmotic response but maintained the TNF-alpha response. Electrophoretic gel mobility shift assays showed two NF-kappa B proteins, p50 and p52, capable of binding ORE sequence, and gel shift Western assay detected NF-kappa B proteins p50 and p65 in the ORE complex. Inhibitors of NF-kappa B signaling, lactacystin, and MG132 abolished the AR promoter response to TNF-alpha. C1 NEI, NIH, Lab Mechanisms Ocular Dis, Bethesda, MD 20892 USA. NEI, Lab Ocular Therapeut, Bethesda, MD 20892 USA. NICHD, Lab Mol Growth Regulat, NIH, Bethesda, MD 20892 USA. Nippon Med Sch, Dept Ophthalmol, Bunkyo Ku, Tokyo 113, Japan. Univ Michigan, Sch Med, Kellogg Eye Ctr, Ann Arbor, MI 48105 USA. RP Carper, D (reprint author), NEI, NIH, Lab Mechanisms Ocular Dis, 9000 Rockville Pike,Bldg 6,Rm232, Bethesda, MD 20892 USA. NR 41 TC 55 Z9 55 U1 2 U2 2 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 19 PY 1999 VL 274 IS 12 BP 7993 EP 8001 DI 10.1074/jbc.274.12.7993 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178LU UT WOS:000079268100057 PM 10075698 ER PT J AU Jayadev, S Petranka, JG Cheran, SK Biermann, JA Barrett, JC Murphy, E AF Jayadev, S Petranka, JG Cheran, SK Biermann, JA Barrett, JC Murphy, E TI Reduced capacitative calcium entry correlates with vesicle accumulation and apoptosis SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article ID SIGNAL-TRANSDUCTION PATHWAYS; PROTEIN-KINASE-C; INTRACELLULAR CALCIUM; CA2+ INFLUX; NEOPLASTIC PROGRESSION; CELL-LINE; DNA FRAGMENTATION; STORE DEPLETION; XENOPUS OOCYTES; LEUKEMIA-CELLS AB A preneoplastic variant of Syrian hamster embryo cells, sup(+), exhibits decreased endoplasmic reticulum calcium levels and subsequently undergoes apoptosis in low serum conditions (Preston, G, A, Barrett, J, C,, Biermann, J, A, and Murphy, E, (1997) Cancer Res. 57, 537-542). This decrease in endoplasmic reticulum calcium appears to be due, at least in part, to reduced capacitative calcium entry at the plasma membrane. Thus we investigated whether inhibition of capacitative calcium entry per se could reduce endoplasmic reticulum calcium and induce apoptosis of cells. We find that treatment with either SKF96365 (30-100 mu M) Or cell-impermeant 1,2-bis(o-amino-5-bromophenoxy)ethane-N,N,N',N'-tetra-acetic acid (5-10 mM) is able to induce apoptosis of cells in conditions where apoptosis does not normally occur. Because previous work has implicated vesicular trafficking as a mechanism of regulating capacitative calcium entry, we investigated whether disruption of vesicular trafficking could lead to decreased capacitative calcium entry and subsequent apoptosis of cells. Coincident with low serum-induced apoptosis, we observed an accumulation of vesicles within the cell, suggesting deregulated vesicle trafficking, Treatment of cells with bafilomycin (30-100 nM), an inhibitor of the endosomal proton ATPase, produced an accumulation of vesicles, decreased capacitative entry, and induced apoptosis, These data suggest that deregulation of vesicular transport results in reduced capacitative calcium entry which in turn results in apoptosis. C1 NIEHS, Mol Carcinogenesis Lab, Res Triangle Pk, NC 27709 USA. RP Jayadev, S (reprint author), NIEHS, Mol Carcinogenesis Lab, POB 12233,MD D2-03, Res Triangle Pk, NC 27709 USA. EM jayadev@niehs.nih.gov NR 76 TC 32 Z9 32 U1 0 U2 1 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0021-9258 J9 J BIOL CHEM JI J. Biol. Chem. PD MAR 19 PY 1999 VL 274 IS 12 BP 8261 EP 8268 DI 10.1074/jbc.274.12.8261 PG 8 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 178LU UT WOS:000079268100091 PM 10075732 ER PT J AU Urbaneja, MA Kane, BP Johnson, DG Gorelick, RJ Henderson, LE Casas-Finet, JR AF Urbaneja, MA Kane, BP Johnson, DG Gorelick, RJ Henderson, LE Casas-Finet, JR TI Binding properties of the human immunodeficiency virus type 1 nucleocapsid protein p7 to a model RNA: Elucidation of the structural determinants for function SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE HIV-1 nucleocapsid protein; zinc fingers; RNA binding; thermodynamics of nucleic acid binding; NCp7 structural determinants ID MURINE LEUKEMIA-VIRUS; DETECTED MAGNETIC-RESONANCE; TERMINAL ZINC-FINGER; AMINO-ACID-RESIDUES; CYS-HIS BOX; IN-VITRO; REVERSE-TRANSCRIPTASE; VIRAL-RNA; ANNEALING ACTIVITIES; STRAND TRANSFER AB HIV-1 nucleocapsid protein (NCp7) is a double zinc-fingered protein that has been traditionally implicated in viral RNA recognition and packaging, in addition to its tight association with genomic RNA and tRNA primer within the virion nucleocapsid. The availability of large quantities of viral or recombinant wild-type NCp7 and mutant p7 has made possible the assignment of the different roles that structural motifs within the protein play during RNA binding. At low ionic strength binding to the homopolymeric fluorescent RNA, poly(epsilon A), is electrostatically driven and four sodium ions are displaced. Arg7 in the flanking N-terminal region, Lys20 and Lys26 in the first zinc finger and one positively charged residue (attributed to Lys41) in the second zinc finger are involved in electrostatic contacts with RNA. The p7 zinc fingers do not function independently but concomitantly. The first zinc finger (both isolated or in the context of the full-length protein) has a more prominent electrostatic interaction than the second one. The second zinc finger dominates the non-electrostatic stabilization of the binding to RNA due to stacking of its Trp residue with nucleic acid bases. Mutations in the highly conserved retroviral Zn-coordinating residues (CCHC) to steroid hormone receptor (CCCC) or transcription factor (CCHH) metal cluster types do not affect RNA binding. Ln spite of the limited impact in RNA binding affinity in vitro or RNA packaging in vivo that such mutations or structural alterations impart, they impair or abolish virus infectivity. It is likely that such an effect stems from the involvement of NCp7 in crucial steps of the virus life cycle other than RNA binding. (C) 1999 Academic Press. C1 NCI, AIDS Vaccine Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Univ Basque Country, Fac Ciencias, Dept Bioquim & Biol Mol, Grp Biomembranas,CSIC,Unidad Asociada, E-48080 Bilbao, Spain. RP Urbaneja, MA (reprint author), NCI, AIDS Vaccine Program, SAIC Frederick, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. EM urbaneja@avpaxpl.ncifcrf.gov FU NCI NIH HHS [N01-CO-56000] NR 79 TC 74 Z9 75 U1 1 U2 5 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD MAR 19 PY 1999 VL 287 IS 1 BP 59 EP 75 DI 10.1006/jmbi.1998.2521 PG 17 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 179FP UT WOS:000079315400006 PM 10074407 ER PT J AU Subramaniam, S Lindahl, I Bullough, P Faruqi, AR Tittor, J Oesterhelt, D Brown, L Lanyi, J Henderson, R AF Subramaniam, S Lindahl, I Bullough, P Faruqi, AR Tittor, J Oesterhelt, D Brown, L Lanyi, J Henderson, R TI Protein conformational changes in the bacteriorhodopsin photocycle SO JOURNAL OF MOLECULAR BIOLOGY LA English DT Article DE electron crystallography; seven-helix membrane protein; proton pump; conformational change; trapped intermediates ID X-RAY-DIFFRACTION; TRANSFORM INFRARED-SPECTRA; INTERNAL PROTON DONOR; STRUCTURAL-CHANGES; N-INTERMEDIATE; SCHIFF-BASE; ELECTRON-DIFFRACTION; ASPARTIC ACID-96; HALOBACTERIUM-HALOBIUM; CHROMOPHORE STRUCTURE AB We report a comprehensive electron crystallographic analysis of conformational changes in the photocycle of wild-type bacteriorhodopsin and in a variety of mutant proteins with kinetic defects in the photocycle. Specific intermediates that accumulate in the late stages of the photocycle of wildtype bacteriorhodopsin, the single mutants D38R, D96N, D96G, T46V, L93A and F219L, and the triple mutant D96G/F171C/3F219L were trapped by freezing two-dimensional crystals in liquid ethane at varying times after illumination with a light flash. Electron diffraction patterns recorded from these crystals were used to construct projection difference Fourier maps at 3.5 Angstrom resolution to define light-driven changes in protein conformation. Our experiments demonstrate that in wild-type bacteriorhodopsin, a large protein conformational change occurs within similar to 1 ms after illumination. Analysis of structural changes in wild-type and mutant bacteriorhodopsins under conditions when either the M or the N intermediate is preferentially accumulated reveals that there are only small differences in structure between M and N intermediates trapped in the same protein. However, a considerably larger variation is observed when the same optical intermediate is trapped in different mutants. In some of the mutants, a partial conformational change is present even prior to illumination, with additional changes occurring upon illumination. Selected mutations, such as those in the D96G/F171C/F219L triple mutant, can sufficiently destabilize the wild-type structure to generate almost the full extent of the conformational change in the dark, with minimal additional Fight-induced changes. We conclude that the differences in structural changes observed in mutants that display long-lived M, N or O intermediates are best described as variations of one fundamental type of conformational change, rather than representing structural changes that are unique to the optical intermediate that is accumulated. Our observations thus support a simplified view of the photocycle of wild-type bacteriorhodopsin in which the structures of the initial state and the early intermediates (K, L, and M,) are well approximated by one protein conformation, while the structures of the later intermediates (M-2, N and O) are well approximated by the other protein conformation. We propose that in wild-type bacteriorhodopsin and in most mutants, this conformational change between the M-1 and M-2 states is likely to make an important contribution towards efficiently switching proton accessibility of the Schiff base from the extracellular side to the cytoplasmic side of the membrane. (C) 1999 Academic Press. C1 MRC, Mol Biol Lab, Cambridge, England. Max Planck Inst Biochem, Martinsried, Germany. Univ Calif Irvine, Irvine, CA 92717 USA. RP Subramaniam, S (reprint author), NCI, Biochem Lab, Bethesda, MD 20892 USA. EM sriram@mrc-lmb.cam.ac; rh15@mrc-lmb.cam.ac.uk RI Brown, Leonid/A-1050-2008 OI Brown, Leonid/0000-0002-5614-8317 NR 61 TC 206 Z9 210 U1 1 U2 9 PU ACADEMIC PRESS LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0022-2836 J9 J MOL BIOL JI J. Mol. Biol. PD MAR 19 PY 1999 VL 287 IS 1 BP 145 EP 161 DI 10.1006/jmbi.1999.2589 PG 17 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 179FP UT WOS:000079315400012 PM 10074413 ER PT J AU Li, CY Peoples, RW Lanthorn, TH Li, ZW Weight, FF AF Li, CY Peoples, RW Lanthorn, TH Li, ZW Weight, FF TI Distinct ATP-activated currents in different types of neurons dissociated from rat dorsal root ganglion SO NEUROSCIENCE LETTERS LA English DT Article DE adenosine 5 '-triphosphate; receptor; neuron; dorsal root ganglion; ion channel; P2X purinoceptor; capsaicin ID FAST SYNAPTIC TRANSMISSION; GATED ION CHANNELS; MAMMALIAN NEURONS; SENSORY NEURONS; RECEPTORS; NODOSE AB Rat dorsal root ganglion neurons can be classified into at least three distinct groups based on cell size, afferent fiber diameter, electrophysiological properties, sensitivity to vanilloid agonists such as capsaicin, and function. In the present study, ATP-activated current in these neurons was characterized using whole-cell patch-clamp recording. Small diameter (<30 mu m) cells had high capsaicin sensitivity, high affinity for ATP, and rapidly desensitizing ATP-activated current. Medium diameter (30-50 mu m) cells had no capsaicin sensitivity, lower affinity for ATP and slowly desensitizing ATP-activated current. Large diameter (>50 mu m) cells were insensitive to both capsaicin and ATP, These findings suggest that distinct types of ATP receptor-ion channels are expressed in different types of dorsal root ganglion neurons, and may contribute to the functional differences among these types of neurons. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved. C1 NIAAA, Mol & Cellular Neurobiol Lab, NIH, Bethesda, MD 20892 USA. Astra Arcus USA, Dept Cell Biol, Rochester, NY 14534 USA. Tongji Med Univ, Res Ctr Expt Med, Dept Mol & Cellular Neurobiol, Wuhan 430030, Peoples R China. RP Li, CY (reprint author), Astra Arcus USA, Dept Cell Biol, 1 Innovat Dr, Worcester, MA 01615 USA. NR 20 TC 39 Z9 45 U1 0 U2 0 PU ELSEVIER SCI IRELAND LTD PI CLARE PA CUSTOMER RELATIONS MANAGER, BAY 15, SHANNON INDUSTRIAL ESTATE CO, CLARE, IRELAND SN 0304-3940 J9 NEUROSCI LETT JI Neurosci. Lett. PD MAR 19 PY 1999 VL 263 IS 1 BP 57 EP 60 DI 10.1016/S0304-3940(99)00114-7 PG 4 WC Neurosciences SC Neurosciences & Neurology GA 178JG UT WOS:000079262100015 PM 10218910 ER PT J AU Lanza, RP Arrow, KJ Axelrod, J Baltimore, D Benacerraf, B Bloch, KE Bloembergen, N Brown, HC Brown, MS Cibelli, JB Cohen, S Cooper, LN Corey, EJ Dulbecco, R Fischer, EH Fitch, VL Friedman, JI Friedman, M Furchgott, RF Gell-Mann, M Glaser, DA Glashow, SL Gilbert, W Goldstein, JL Gould, SJ Guillemin, R Hauptman, HK Hauptman, HA Herschbach, D Hoffman, R Hood, L Hubel, DH Karle, J Klein, LR Kohn, W Kornberg, A Krebs, EG Lederman, LM Lederberg, J Lee, DM Lucas, RE Marcus, RA Merrifield, RB Miller, MH Modigliani, F Molina, MJ Mullis, K Murad, F Murray, JE Nathans, D Nirenberg, MW North, DC Olah, GA Palade, GE Perl, MJ Ramsey, NF Richter, B Roberts, RJ Robl, JM Samuelson, PA Schwartz, M Sharp, PA Smalley, RE Smith, HO Solow, RM Taube, H Tonegawa, S Watson, JD Weinberg, S Weller, TH West, MD Wieschaus, EF Wiesel, TN Wilson, RW AF Lanza, RP Arrow, KJ Axelrod, J Baltimore, D Benacerraf, B Bloch, KE Bloembergen, N Brown, HC Brown, MS Cibelli, JB Cohen, S Cooper, LN Corey, EJ Dulbecco, R Fischer, EH Fitch, VL Friedman, JI Friedman, M Furchgott, RF Gell-Mann, M Glaser, DA Glashow, SL Gilbert, W Goldstein, JL Gould, SJ Guillemin, R Hauptman, HK Hauptman, HA Herschbach, D Hoffman, R Hood, L Hubel, DH Karle, J Klein, LR Kohn, W Kornberg, A Krebs, EG Lederman, LM Lederberg, J Lee, DM Lucas, RE Marcus, RA Merrifield, RB Miller, MH Modigliani, F Molina, MJ Mullis, K Murad, F Murray, JE Nathans, D Nirenberg, MW North, DC Olah, GA Palade, GE Perl, MJ Ramsey, NF Richter, B Roberts, RJ Robl, JM Samuelson, PA Schwartz, M Sharp, PA Smalley, RE Smith, HO Solow, RM Taube, H Tonegawa, S Watson, JD Weinberg, S Weller, TH West, MD Wieschaus, EF Wiesel, TN Wilson, RW TI Science over politics SO SCIENCE LA English DT Letter C1 Stanford Univ, Sch Med, Stanford, CA 94309 USA. NIMH, Bethesda, MD 20892 USA. CALTECH, Pasadena, CA 91125 USA. Dana Farber Canc Inst, Boston, MA 02215 USA. Harvard Univ, Sch Med, Cambridge, MA 02138 USA. Purdue Univ, W Lafayette, IN 47907 USA. Univ Texas, SW Med Ctr, Dallas, TX 75235 USA. Adv Cell Technol, Worcester, MA 01605 USA. Vanderbilt Univ, Sch Med, Nashville, TN 37232 USA. Brown Univ, Providence, RI 02912 USA. Salk Inst Biol Studies, La Jolla, CA 92037 USA. Univ Washington, Seattle, WA 98195 USA. Princeton Univ, Princeton, NJ 08544 USA. MIT, Cambridge, MA 02139 USA. Stanford Univ, Hoover Inst, Stanford, CA 94305 USA. SUNY, Brooklyn, NY 11203 USA. Univ Calif Berkeley, Berkeley, CA 94720 USA. Hauptman Woodward Med Res, Buffalo, NY 14203 USA. Cornell Univ, Ithaca, NY 14853 USA. Univ Penn, Philadelphia, PA 19104 USA. Univ Calif Santa Barbara, Santa Barbara, CA 93106 USA. IIT, Chicago, IL 60616 USA. Rockefeller Univ, New York, NY 10021 USA. Univ Chicago, Chicago, IL 60637 USA. Univ Texas, Sch Med, Houston, TX 75225 USA. Johns Hopkins Univ, Baltimore, MD 21205 USA. NHLBI, Bethesda, MD 20892 USA. Washington Univ, St Louis, MO 63130 USA. Univ So Calif, Los Angeles, CA 90007 USA. Univ Calif San Diego, La Jolla, CA 92093 USA. New England Biolabs Inc, Beverly, MA 01915 USA. Univ Massachusetts, Amherst, MA 01003 USA. Rice Univ, Houston, TX 77005 USA. Johns Hopkins Sch Med, Baltimore, MD 21205 USA. Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. Univ Texas, Austin, TX 78712 USA. Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. Princeton Univ, Princeton, NJ 08544 USA. Rockefeller Univ, New York, NY 10021 USA. Harvard Smithsonian Ctr Astrophys, Cambridge, MA 02138 USA. EM BobLanza@aol.com NR 0 TC 6 Z9 6 U1 11 U2 28 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD MAR 19 PY 1999 VL 283 IS 5409 BP 1849 EP 1850 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177UC UT WOS:000079228600016 PM 10206888 ER PT J AU Paus, T Zijdenbos, A Worsley, K Collins, DL Blumenthal, J Giedd, JN Rapoport, JL Evans, AC AF Paus, T Zijdenbos, A Worsley, K Collins, DL Blumenthal, J Giedd, JN Rapoport, JL Evans, AC TI Structural maturation of neural pathways in children and adolescents: In vivo study SO SCIENCE LA English DT Article ID HUMAN BRAIN-DEVELOPMENT; CENTRAL NERVOUS-SYSTEM; MAGNETIC STIMULATION; CORPUS-CALLOSUM; AUDITORY-CORTEX; MOTOR CORTEX; MYELINATION; ADULTHOOD; SPEECH; SCHIZOPHRENIA AB Structural maturation of fiber tracts in the human brain, including an increase in the diameter and myelination of axons, may play a role in cognitive development during childhood and adolescence. A computational analysis of structural magnetic resonance images obtained in 111 children and adolescents revealed age-related increases in white matter density in fiber tracts constituting putative corticospinal and frontotemporal pathways. The maturation of the corticospinal tract was bilateral. whereas that of the frontotemporal pathway was found predominantly in the left (speech-dominant) hemisphere. These findings provide evidence for a gradual maturation, during late childhood and adolescence, of fiber pathways presumably supporting motor and speech functions. C1 McGill Univ, Montreal Neurol Inst, Montreal, PQ H3A 2B4, Canada. NIMH, Clin Psychiat Branch, Bethesda, MD 20892 USA. RP Paus, T (reprint author), McGill Univ, Montreal Neurol Inst, 3801 Univ St, Montreal, PQ H3A 2B4, Canada. EM tomas@bic.mni.mcgill.ca RI Giedd, Jay/A-3080-2008; Giedd, Jay/B-7302-2012; Giedd, Jay/J-9644-2015 OI Giedd, Jay/0000-0003-0827-3460; Giedd, Jay/0000-0003-2002-8978 NR 45 TC 774 Z9 786 U1 5 U2 48 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD MAR 19 PY 1999 VL 283 IS 5409 BP 1908 EP 1911 DI 10.1126/science.283.5409.1908 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177UC UT WOS:000079228600046 PM 10082463 ER PT J AU Agarwala, R Bafna, V Farach, M Paterson, M Thorup, M AF Agarwala, R Bafna, V Farach, M Paterson, M Thorup, M TI On the approximability of numerical taxonomy (fitting distances by tree metrics) SO SIAM JOURNAL ON COMPUTING LA English DT Article DE approximation algorithm; tree metric; taxonomy AB We consider the problem of fitting an n x n distance matrix D by a tree metric T. Let epsilon be the distance to the closest tree metric under the L-infinity norm; that is, epsilon = min(T) {parallel to T ? D parallel to infinity}. First we present an O(n(2)) algorithm for finding a tree metric T such that parallel to T ? D parallel to infinity less than or equal to 3 epsilon. Second we show that it is NP-hard to find a tree metric T such that parallel to T ? D parallel to infinity < 9/8 epsilon. This paper presents the first algorithm for this problem with a performance guarantee. C1 Rutgers State Univ, DIMACS, Piscataway, NJ 08855 USA. Rutgers State Univ, Dept Comp Sci, Piscataway, NJ 08855 USA. Univ Warwick, Dept Comp Sci, Coventry CV4 7AL, W Midlands, England. Univ Copenhagen, Dept Comp Sci, DK-2100 Copenhagen O, Denmark. RP Agarwala, R (reprint author), Natl Human Genome Res Inst, NIH, Bethesda, MD 20892 USA. OI Thorup, Mikkel/0000-0001-5237-1709 NR 10 TC 54 Z9 54 U1 1 U2 2 PU SIAM PUBLICATIONS PI PHILADELPHIA PA 3600 UNIV CITY SCIENCE CENTER, PHILADELPHIA, PA 19104-2688 USA SN 0097-5397 J9 SIAM J COMPUT JI SIAM J. Comput. PD MAR 19 PY 1999 VL 28 IS 3 BP 1073 EP 1085 PG 13 WC Computer Science, Theory & Methods; Mathematics, Applied SC Computer Science; Mathematics GA 180ZE UT WOS:000079415500011 ER PT J AU Overpeck, MD Trumble, AC Berendes, HW Brenner, RA AF Overpeck, MD Trumble, AC Berendes, HW Brenner, RA TI Risk factors for infant homicide - Reply SO NEW ENGLAND JOURNAL OF MEDICINE LA English DT Letter C1 NICHHD, Bethesda, MD 20892 USA. RP Overpeck, MD (reprint author), NICHHD, Bethesda, MD 20892 USA. NR 4 TC 1 Z9 1 U1 0 U2 0 PU MASSACHUSETTS MEDICAL SOC PI WALTHAM PA WALTHAM WOODS CENTER, 860 WINTER ST,, WALTHAM, MA 02451-1413 USA SN 0028-4793 J9 NEW ENGL J MED JI N. Engl. J. Med. PD MAR 18 PY 1999 VL 340 IS 11 BP 895 EP 896 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 176ZW UT WOS:000079183200030 ER PT J AU Brenner, RA Willinger, M Simons-Morton, BG Hoffman, HJ Clemens, JD AF Brenner, RA Willinger, M Simons-Morton, BG Hoffman, HJ Clemens, JD TI Putting babies "Back to Sleep" - Reply SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Letter ID INFANT-DEATH-SYNDROME C1 NIH, Bethesda, MD 20892 USA. RP Brenner, RA (reprint author), NIH, Bldg 10, Bethesda, MD 20892 USA. NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER MEDICAL ASSOC PI CHICAGO PA 515 N STATE ST, CHICAGO, IL 60610 USA SN 0098-7484 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD MAR 17 PY 1999 VL 281 IS 11 BP 983 EP 984 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 175EE UT WOS:000079079400014 ER PT J AU Appella, DH Barchi, JJ Durell, SR Gellman, SH AF Appella, DH Barchi, JJ Durell, SR Gellman, SH TI Formation of short, stable helices in aqueous solution by beta-amino acid hexamers SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID SECONDARY STRUCTURE; PEPTIDE FOLDAMERS; OLIGOMERS; SPECTROSCOPY; PROTEIN; NMR; STABILITY; SEQUENCE; SPECTRA; FAMILY C1 NCI, Med Chem Lab, Bethesda, MD 20892 USA. NCI, Lab Expt & Computat Biol, Div Basic Sci, Bethesda, MD 20892 USA. Univ Wisconsin, Dept Chem, Madison, WI 53706 USA. RP Barchi, JJ (reprint author), NCI, Med Chem Lab, Bethesda, MD 20892 USA. RI Barchi Jr., Joseph/N-3784-2014 NR 43 TC 193 Z9 193 U1 1 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD MAR 17 PY 1999 VL 121 IS 10 BP 2309 EP 2310 DI 10.1021/ja983918n PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 178AG UT WOS:000079242800036 ER PT J AU Kuszewski, J Gronenborn, AM Clore, GM AF Kuszewski, J Gronenborn, AM Clore, GM TI Improving the packing and accuracy of NMR structures with a pseudopotential for the radius of gyration SO JOURNAL OF THE AMERICAN CHEMICAL SOCIETY LA English DT Article ID IMMUNOGLOBULIN-BINDING DOMAIN; STREPTOCOCCAL PROTEIN-G C1 NIDDKD, Phys Chem Lab, NIH, Bethesda, MD 20892 USA. RP Clore, GM (reprint author), NIDDKD, Phys Chem Lab, NIH, Bldg 5, Bethesda, MD 20892 USA. EM clore@speck.niddk.nih.gov RI Clore, G. Marius/A-3511-2008 OI Clore, G. Marius/0000-0003-3809-1027 NR 16 TC 184 Z9 187 U1 0 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0002-7863 J9 J AM CHEM SOC JI J. Am. Chem. Soc. PD MAR 17 PY 1999 VL 121 IS 10 BP 2337 EP 2338 DI 10.1021/ja9843730 PG 2 WC Chemistry, Multidisciplinary SC Chemistry GA 178AG UT WOS:000079242800050 ER PT J AU Ballard-Barbash, R Forman, MR Kipnis, V AF Ballard-Barbash, R Forman, MR Kipnis, V TI Dietary fat, serum estrogen levels, and breast cancer risk: a multifaceted story SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Editorial Material ID PREMENOPAUSAL WOMEN; HORMONE LEVELS; PLASMA; CONSUMPTION C1 NCI, Appl Res Branch, Div Canc Control & Populat Sci, NIH, Bethesda, MD 20892 USA. NCI, Canc Prevent Studies Branch, Div Clin Sci, NIH, Bethesda, MD 20892 USA. NCI, Biometry Branch, Div Canc Prevent, NIH, Bethesda, MD 20892 USA. RP Ballard-Barbash, R (reprint author), NCI, Appl Res Branch, Div Canc Control & Populat Sci, NIH, Execut Plaza N,Rm 313, Bethesda, MD 20892 USA. NR 16 TC 10 Z9 10 U1 0 U2 1 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 17 PY 1999 VL 91 IS 6 BP 492 EP 494 DI 10.1093/jnci/91.6.492 PG 3 WC Oncology SC Oncology GA 177WY UT WOS:000079235100002 PM 10088613 ER PT J AU James, K Eisenhauer, E Christian, M Terenziani, M Vena, D Muldal, A Therasse, P AF James, K Eisenhauer, E Christian, M Terenziani, M Vena, D Muldal, A Therasse, P TI Measuring response in solid tumors: Unidimensional versus bidimensional measurement SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID ONCOLOGY; CRITERIA; CANCER AB Background: Tumor shrinkage is a common end point used in screening new cytotoxic agents. The standard World Health Organization criterion for partial response is a 50% or more decrease in the sum of the products of two measurements (the maximum diameter of a tumor and the largest diameter perpendicular to this maximum diameter) of individual tumors. However, theoretically, the simple sum of the maximum diameters of individual tumors is more linearly related to cell kill than is the sum of the bidimensional products. It has been hypothesized that the calculation of bidimensional products is unnecessary, and a 30% decrease in the sum of maximum diameters of individual tumors (assuming spherical shape and equivalence to a 50% reduction in the sum of the bidimensional products) was proposed as a new criterion. We have applied the standard response and the new response criteria to the same data to determine whether the same number of responses in the same patients would result. Methods: Data from 569 patients included in eight studies of a variety of cancers were reanalyzed. The two response criteria were separately applied, and the results were compared using the kappa statistic. The importance of confirmatory measurements and the frequency of nonspherical tumors were also examined. In addition, for a subset of 128 patients, a unidimensional criterion for disease progression (30% increase in the sum of maximum diameters) was applied and compared with the standard definition of a 25% increase in the sum of the bidimensional products. Results: Agreement between the unidimensional and bidimensional criteria was generally found to be good. The kappa statistic for concordance for overall response was 0.95. Conclusion: We conclude that one dimensional measurement of tumor maximum diameter may be sufficient to assess change in solid tumors. C1 Queens Univ, Natl Canc Inst Canada, Clin Trials Grp, Kingston, ON K7L 3N6, Canada. NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA. NCI, Div Med Oncol, Milan, Italy. Emmes Corp, Rockville, MD USA. European Org Res & Treatment Canc, Ctr Data, Brussels, Belgium. RP James, K (reprint author), Queens Univ, Natl Canc Inst Canada, Clin Trials Grp, 82-84 Barrie St, Kingston, ON K7L 3N6, Canada. EM jamesk@ncic.ctg.queensu.ca RI Terenziani, Monica/B-9562-2017 OI Terenziani, Monica/0000-0002-7080-6718 NR 11 TC 269 Z9 294 U1 0 U2 3 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 17 PY 1999 VL 91 IS 6 BP 523 EP 528 DI 10.1093/jnci/91.6.523 PG 6 WC Oncology SC Oncology GA 177WY UT WOS:000079235100011 PM 10088622 ER PT J AU Stolzenberg-Solomon, RZ Albanes, D Nieto, FJ Hartman, TJ Tangrea, JA Rautalahti, M Sehlub, J Virtamo, J Taylor, PR AF Stolzenberg-Solomon, RZ Albanes, D Nieto, FJ Hartman, TJ Tangrea, JA Rautalahti, M Sehlub, J Virtamo, J Taylor, PR TI Pancreatic cancer risk and nutrition-related methyl-group availability indicators in male smokers SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID METHYLENETETRAHYDROFOLATE REDUCTASE POLYMORPHISM; NEURAL-TUBE DEFECTS; FOLATE-DEFICIENCY; COLORECTAL-CANCER; LIQUID-CHROMATOGRAPHY; PLASMA; CARCINOGENESIS; CHOLECYSTOKININ; HOMOCYSTEINE; CARCINOMAS AB Background: Few risk factors for pancreatic cancer have been identified, with age and cigarette smoking being the most consistent. The protective effect associated with consumption of fruits and vegetables-the major dietary sources of folate-is suggestive of a role for factors influencing cellular methylation reactions; however, to our knowledge, no study has investigated this relationship. Whether biochemical indicators of methyl-group availability are associated with exocrine pancreatic cancer risk was the focus of this investigation. Methods: We conducted a nested case-control study within the Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study cohort of 29133 male Finnish smokers aged 50-69 years. One hundred twenty-six subjects with incident exocrine pancreatic cancer were matched by date of baseline blood draw (+/-30 days), study center, age (+/-5 years), trial intervention group, and completion of dietary history to 247 control subjects, who were alive and free from cancer at the time the case subjects were diagnosed. Odds ratios (ORs) and 95% confidence intervals (CIs) were determined by use of conditional logistic regression. Reported P values are two-tailed. Results: Serum folate and pyridoxal-5'-phosphate (PLP) concentrations showed statistically significant inverse dose-response relationships with pancreatic cancer risk, with the highest serum tertiles having approximately half the risk of the lowest (folate: OR = 0.45; 95% CI = 0.24-0.82; P for trend =.009, and PLP: OR = 0.48; 95% CI = 0.26-0.88; P for trend =.02), An increased pancreatic cancer risk was also observed with greater exposure to cigarettes (e.g., pack-years [number of packs smoked per day x number of years of smoking], highest versus lowest quartile: OR = 2.13; 95% CP = 1.13-3.99; P for trend =.04). Conclusions: These results support the hypothesis that maintaining adequate folate and pyridoxine status may reduce the risk of pancreatic cancer and confirm the risk previously associated with cigarette smoking. C1 NCI, Canc Prevent Studies Branch, Div Clin Sci, NIH, Bethesda, MD 20892 USA. Johns Hopkins Univ, Sch Hyg & Publ Hlth, Dept Epidemiol, Baltimore, MD USA. Natl Publ Hlth Inst, Helsinki, Finland. Tufts Univ, USDA, Jean Mayer Human Nutr Res Ctr, Boston, MA USA. RP Stolzenberg-Solomon, RZ (reprint author), NCI, Canc Prevent Studies Branch, Div Clin Sci, NIH, 6006 Execut Blvd,Suite 321, Bethesda, MD 20892 USA. EM RS221Z@NIH.GOV RI Albanes, Demetrius/B-9749-2015 FU NCI NIH HHS [N01CN45165] NR 60 TC 104 Z9 112 U1 0 U2 1 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 17 PY 1999 VL 91 IS 6 BP 535 EP 541 DI 10.1093/jnci/91.6.535 PG 7 WC Oncology SC Oncology GA 177WY UT WOS:000079235100013 PM 10088624 ER PT J AU Zhang, SM Hunter, DJ Forman, MR Rosner, BA Speizer, FE Colditz, GA Manson, JE Hankinson, SE Willett, WC AF Zhang, SM Hunter, DJ Forman, MR Rosner, BA Speizer, FE Colditz, GA Manson, JE Hankinson, SE Willett, WC TI Dietary carotenoids and vitamins A, C, and E and risk of breast cancer SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Article ID ALPHA-TOCOPHEROL LEVELS; BETA-CAROTENE; ALCOHOL-CONSUMPTION; FAMILY HISTORY; NEW-YORK; VEGETABLES; QUESTIONNAIRE; FRUITS; COHORT; VALIDATION AB Background: Data on intake of specific carotenoids and breast cancer risk are limited. Furthermore, studies of vitamins A, C, and E in relation to breast cancer risk are inconclusive, We have conducted a large, prospective study to evaluate long-term intakes of these nutrients and breast cancer risk. Methods: We examined, by use of multivariate analysis, associations between intakes of specific carotenoids, vitamins A, C, and F, consumption of fruits and vegetables, and breast cancer risk in a cohort of 83 234 women (aged 33-60 years in 1980) who were participating in the Nurses' Health Study. Through 1994, we identified 2697 incident cases of invasive breast cancer (784 premenopausal and 1913 postmenopausal). Results: Intakes of beta-carotene from food and supplements, lutein/zeaxanthin, and vitamin A from foods were weakly inversely associated with breast cancer risk in premenopausal women. Strong inverse associations were found for increasing quintiles of alpha-carotene, beta-carotene, lutein/zeaxanthin, total vitamin C from foods, and total vitamin A among premenopausal women with a positive family history of breast cancer. An inverse association was also found for increasing quintiles of beta-carotene among premenopausal women who consumed 15 g or more of alcohol per day. Premenopausal women who consumed five or more servings per day of fruits and vegetables had modestly lower risk of breast cancer than those who had less than two servings per day (relative risk [RR] = 0.77; 95% confidence interval [CI] = 0.58-1.02); this association was stronger among premenopausal women who had a positive family history of breast cancer (RR = 0.29; 95% CI = 0.13-0.62) or those who consumed 15 g or more of alcohol per day (RR = 0.53; 95% CI = 0.27-1.04). Conclusions: Consumption of fruits and vegetables high in specific carotenoids and vitamins may reduce premenopausal breast cancer risk. C1 Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. Harvard Univ, Sch Med, Dept Med, Channing Lab, Boston, MA USA. Harvard Univ, Sch Publ Hlth, Harvard Ctr Canc Prevent, Boston, MA 02115 USA. NCI, Div Clin Sci, Bethesda, MD 20892 USA. Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. Harvard Univ, Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02115 USA. Harvard Univ, Sch Med, Dept Med, Div Prevent Med, Boston, MA USA. Brigham & Womens Hosp, Boston, MA 02115 USA. RP Zhang, SM (reprint author), Harvard Univ, Sch Publ Hlth, Dept Nutr, 665 Huntington Ave, Boston, MA 02115 USA. RI Colditz, Graham/A-3963-2009 OI Colditz, Graham/0000-0002-7307-0291 FU NCI NIH HHS [CA40356] NR 42 TC 240 Z9 248 U1 0 U2 16 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 17 PY 1999 VL 91 IS 6 BP 547 EP 556 DI 10.1093/jnci/91.6.547 PG 10 WC Oncology SC Oncology GA 177WY UT WOS:000079235100015 PM 10088626 ER PT J AU Frisch, M Hjalgrim, H AF Frisch, M Hjalgrim, H TI Nonmelanomatous skin cancer following cervical, vaginal, and vulvar neoplasms: Etiologic association SO JOURNAL OF THE NATIONAL CANCER INSTITUTE LA English DT Letter ID RISK C1 Statens Serum Inst, Dept Epidemiol Res, Danish Epidemiol Sci Ctr, DK-2300 Copenhagen, Denmark. RP Frisch, M (reprint author), NCI, Viral Epidemiol Branch, Div Canc Epidemiol & Genet, NIH, Execut Plaza S,Rm 8015, Rockville, MD 20852 USA. RI Frisch, Morten/E-9206-2016 OI Frisch, Morten/0000-0002-3864-8860 NR 7 TC 1 Z9 1 U1 0 U2 0 PU NATL CANCER INSTITUTE PI BETHESDA PA 9030 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0027-8874 J9 J NATL CANCER I JI J. Natl. Cancer Inst. PD MAR 17 PY 1999 VL 91 IS 6 BP 565 EP 566 DI 10.1093/jnci/91.6.565 PG 2 WC Oncology SC Oncology GA 177WY UT WOS:000079235100024 PM 10088632 ER PT J AU Lester, DS Lyon, RC McGregor, GN Engelhardt, RT Schmued, LC Johnson, GA Johannessen, JJ AF Lester, DS Lyon, RC McGregor, GN Engelhardt, RT Schmued, LC Johnson, GA Johannessen, JJ TI 3-Dimensional visualization of lesions in rat brain using magnetic resonance imaging microscopy SO NEUROREPORT LA English DT Article DE 3-D; brain lesions; imaging; microscopy; MRI; neurotoxicity ID DOMOIC ACID; NEURONAL DEGENERATION; NEUROTOXIC LESION; RESOLUTION; HISTOLOGY; POISON AB HIGH-RESOLUTION (< 50 mu m) magnetic resonance imaging microscopy (MRM) has been used to identify brain regions and localization of excitotoxin-induced lesions in fixed rat brains, subsequently confirmed using standard histology. The anatomical extent of lesions identified by MRM Nas identical to that seen in histological sections and various histopathological changes could be visualized. In contrast to the time involved in preparing and examining histological sections, lesions in intact brains could be rapidly identified and visualized in three dimensions by examining digitally generated sections in any plane. This study shows that MRM has tremendous potential as a prescreening tool for neurotoxicity and neuropathology. These observations suggest that MRM has the potential to affect pathology much as conventional MRI has influenced clinical imaging. NeuroReport 10:737-741 (C) 1999 Lippincott Williams & Wilkins. C1 US FDA, Div Appl Pharmacol Res, Laurel, MD 20708 USA. US FDA, Div Prod Qual Res, Ctr Drug Evaluat & Res, Laurel, MD 20708 USA. US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD 20708 USA. NCI, Frederick Biomed Supercomp Ctr, SAIC Frederick, FCRDC, Frederick, MD USA. Duke Univ, Med Ctr, Ctr In Vivo Microscopy, Durham, NC USA. US FDA, Div Neurotoxicol, Natl Ctr Toxicol Res, Jefferson, AR 72079 USA. RP Lester, DS (reprint author), US FDA, Div Appl Pharmacol Res, 8301 Muirkirk Rd,Rm 2009, Laurel, MD 20708 USA. OI Johnson, G.Allan/0000-0002-7606-5447 FU PHS HHS [P41 05959] NR 14 TC 18 Z9 20 U1 0 U2 3 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0959-4965 J9 NEUROREPORT JI Neuroreport PD MAR 17 PY 1999 VL 10 IS 4 BP 737 EP 741 DI 10.1097/00001756-199903170-00014 PG 5 WC Neurosciences SC Neurosciences & Neurology GA 180PA UT WOS:000079393300016 PM 10208540 ER PT J AU Li, CY Wang, H Xue, H Carlier, PR Hui, KM Pang, YP Li, ZW Han, YF AF Li, CY Wang, H Xue, H Carlier, PR Hui, KM Pang, YP Li, ZW Han, YF TI Bis(7)-tacrine, a novel dimeric AChE inhibitor, is a potent GABA(A) receptor antagonist SO NEUROREPORT LA English DT Article DE acetylcholinesterase; bis(7)-tacrine; cholinesterase inhibitor; GABA(A) receptor; muscimol; physostigmine; tacrine ID ACETYLCHOLINE-RELEASE; SITE LIGANDS; BENZODIAZEPINE; MODULATION; MUSCIMOL; ASSAY; RAT AB HEPTYLENE-LINKED bis-(9-amino-1,2,3,4-tetrahydroacridine) (bis(7)-tacrine) is a potential palliative therapeutic agent for Alzheimer's disease (AD), on the basis of its superior acetylcholinesterase (AChE) inhibition and memory-enhancing potency relative to tacrine. In this study we report that bis(7)-tacrine exhibits a potentially complementary central nervous system action, antagonism of GABA(A) receptor function. Bis(7)tacrine displaced [H-3]muscimol from rat brain membranes with an apparent K-i of 6.0 mu M; tacrine and physostigmine were shown to be 18 and 170 times less potent, respectively. In whole-cell patch-clamp recordings, bis(7)-tacrine inhibited GABA-induced inward current with an IC50 of 5.6 mu M, and shifted the GABA concentration-response curve to the right in a parallel manner. These results suggest that bis(7)-tacrine is a competitive antagonist of the GABA(A) receptor. NeuroReport 10:795-800 (C) 1999 Lippincott Williams & Wilkins. C1 Hong Kong Univ Sci & Technol, Dept Biochem, Hong Kong, Peoples R China. Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Peoples R China. NIAAA, Mol & Cellular Neurobiol Lab, NIH, Bethesda, MD 20892 USA. Mayo Clin & Mayo Fdn, Dept Pharmacol, Mayo Canc Ctr, Rochester, MN 55905 USA. Tongji Med Univ, Res Ctr Expt Med, Wuhan 430030, Peoples R China. RP Han, YF (reprint author), Hong Kong Univ Sci & Technol, Dept Biochem, Clear Water Bay, Hong Kong, Peoples R China. OI Carlier, Paul/0000-0002-6683-285X NR 28 TC 26 Z9 29 U1 0 U2 1 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0959-4965 J9 NEUROREPORT JI Neuroreport PD MAR 17 PY 1999 VL 10 IS 4 BP 795 EP 800 DI 10.1097/00001756-199903170-00024 PG 6 WC Neurosciences SC Neurosciences & Neurology GA 180PA UT WOS:000079393300026 PM 10208550 ER PT J AU Juhasz, K Whitehead, SS Boulanger, CA Firestone, CY Collins, PL Murphy, BR AF Juhasz, K Whitehead, SS Boulanger, CA Firestone, CY Collins, PL Murphy, BR TI The two amino acid substitutions in the L protein of cpts530/1009, a live-attenuated respiratory syncytial virus candidate vaccine, are independent temperature-sensitive and attenuation mutations SO VACCINE LA English DT Article DE RSV; live attenuated vaccine ID SERONEGATIVE CHIMPANZEES; GENE-EXPRESSION; TS PHENOTYPE; POLYMERASE; CHILDREN; INFANTS; MUTANT; RSV; IMMUNOGENICITY; GLYCOPROTEINS AB (cpts530/1009 is a live-attenuated, temperature-sensitive (ts) RsV vaccine candidate that was shown previously to be attenuated for seronegative humans. It was generated by two rounds of chemical mutagenesis: first, a partially attenuated, cold-passaged (cp). non-ts RSV mutant (cpRSV) was mutagenized to yield the ts derivative cpts530, and then cpts530 was mutagenized to yield cpts530/1009, which is more ts, Previous nucleotide (nt) sequence analysis of cpts530 showed that it has a single nt change compared to cpRSV that results in an amino acid substitution at residue 521 in the L protein. Reverse genetics confirmed that this mutation is responsible for the ts phenotype of cpts530. Here, determination of the complete 15,222-nt sequence of cpts530/1009 identified a single change compared to cpts530, namely a point mutation at nt 12002, which results in a methionine-to-valine substitution at amino acid 1169 in the L protein. The contribution of the 1009 mutation to the level of temperature sensitivity and attenuation exhibited by cpts530/1009 was evaluated by its introduction alone or with the 530 cp mutations into the full-length cDNA clone of wild-type (wt) RSV, Subsequent analysis of infectious viruses recovered from the mutant cDNAs indicated that ii) the 1009 mutation indeed was a rs mutation and the level of temperature sensitivity specified by the 1009 mutation was less than that specified by the 530 mutation, (ii) the 530 and 1009 mutations each contributed to attenuation in the upper respiratory tract of mice and their effects were additive, (iii) viruses bearing the 1009 mutation were more attenuated in the lower respiratory tract of mice than viruses bearing the 530 mutation and (iv) the combination of the 530 and 1009 mutations in the cpRSV background resulted in the same level of temperature sensitivity and attenuation in mice as that observed for the biologically-derived cpts530/1009 mutant. These data show that the genetic basis of the attenuation and temperature sensitivity of the cpts530/1009 candidate vaccine virus is the sum of the contributions of seven identified amino acid substitutions, i.e. the 5 cpRSV mutations, the 530 mutation and the 1009 mutation, (C) 1999 Elsevier Science Ltd. All rights reserved. C1 NIAID, Infect Dis Lab, NIH, Bethesda, MD 20892 USA. RP Murphy, BR (reprint author), NIAID, Infect Dis Lab, NIH, 7 Ctr Dr,MSC 0720, Bethesda, MD 20892 USA. FU NIAID NIH HHS [AI-000030, AI-000087] NR 22 TC 34 Z9 35 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 17 PY 1999 VL 17 IS 11-12 BP 1416 EP 1424 DI 10.1016/S0264-410X(98)00381-8 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 175YG UT WOS:000079123200017 PM 10195777 ER PT J AU Mrsny, RJ Daugherty, AL Fryling, CM FitzGerald, DJ AF Mrsny, RJ Daugherty, AL Fryling, CM FitzGerald, DJ TI Mucosal administration of a chimera composed of Pseudomonas exotoxin and the gp120 V3 loop sequence of HIV-1 induces both salivary and serum antibody responses SO VACCINE LA English DT Article DE HIV; V3 loop; mucosal vaccine; exotoxin ID HUMAN-IMMUNODEFICIENCY-VIRUS; T-CELL RESPONSES; VACCINE DEVELOPMENT; PEPTIDE VACCINE; INFECTION; IMMUNITY; TYPE-1; PROTEIN; AIDS; NEUTRALIZATION AB We have used a mouse immunization model to evaluate the potential for a chimera protein composed of a nontoxic form of Pseudomonas exotoxin (ntPE) to incite and sustain a mucosal immune response against an integrated antigen. The chimera, termed ntPE-V3MN26, contained 26 amino acids of the gp120 V3 loop region sequence of the MN strain of HIV-1 integrated in place of the Ib region of ntPE. Following either vaginal, rectal, oral or subcutaneous administration and boosting, anti-gp120-specific IgA and IgG levels in serum and saliva samples were assessed by ELISA. All dosing regimens stimulated significant and comparable salivary IgA and serum IgG responses at 1, 2 and 3 months after the initial inoculation. Following a boost at 16 months with ntPE-V3MN26, a strong memory response to the antigen was observed. Isotyping of serum antibodies at this time suggested that both a Th1 and a Th2 response had been induced. Responses to ntPE-V3MN26 following subcutaneous injection in the presence or absence of Freund's adjuvant demonstrated that Freund's adjuvant resulted in a three-fold greater enhancement of immune response compared to administration of chimera alone. These results demonstrate that mucosal presentation of a chimera composed of a nontoxic form of Pseudomonas exotoxin can result in a strong mucosal and systemic antigen-specific immune response to an integrated antigen. The profound memory responses induced by this chimera may be particularly useful for practical vaccine applications. (C) 1999 Elsevier Science Ltd. All rights reserved. C1 Genentech Inc, Dept Pharmaceut Res & Dev, S San Francisco, CA 94080 USA. NCI, Biotherapy Sect, Mol Biol Lab, Div Basic Sci, Bethesda, MD 20892 USA. RP Mrsny, RJ (reprint author), Genentech Inc, Dept Pharmaceut Res & Dev, S San Francisco, CA 94080 USA. NR 47 TC 21 Z9 24 U1 0 U2 0 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD MAR 17 PY 1999 VL 17 IS 11-12 BP 1425 EP 1433 DI 10.1016/S0264-410X(98)00380-6 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 175YG UT WOS:000079123200018 PM 10195778 ER PT J AU Gulick, RM McAuliffe, V Holden-Wiltse, J Crumpacker, C Liebes, L Stein, DS Meehan, P Hussey, S Forcht, J Valentine, FT AF Gulick, RM McAuliffe, V Holden-Wiltse, J Crumpacker, C Liebes, L Stein, DS Meehan, P Hussey, S Forcht, J Valentine, FT CA AIDS Clin Trials Grp 150 258 Protocol TI Phase I studies of hypericin, the active compound in St. John's Wort, as an antiretroviral agent in HIV-infected adults - AIDS clinical trials group protocols 150 and 258 SO ANNALS OF INTERNAL MEDICINE LA English DT Article ID HUMAN-IMMUNODEFICIENCY-VIRUS; PSEUDOHYPERICIN; INACTIVATION AB Background: Hypericin, the active compound in St. John's Wort, has antiretroviral activity in vitro. Many HIV-infected persons use St. John's wort. Objective: To evaluate the safety and antiretroviral activity of hypericin in HIV-infected patients. Design: Phase I study. Setting: Four clinical research units. Patients: 30 HIV-infected patients with CD4 counts less than 350 cells/mm(3). Intervention: Intravenous hypericin, 0.25 or 0.5 mg/kg of body weight twice weekly or 0.25 mg/kg three times weekly, or oral hypericin, 0.5 mg/kg daily. Measurements: Safety was assessed at weekly visits. Antiretroviral activity was assessed by changes in HIV p24 antigen level, HIV titer, HIV RNA copies, and CD4 cell counts. Results: Of the 30 patients who were enrolled, 16 discontinued treatment early because of toxic effects. Severe cutaneous phototoxicity was observed in 11 of 23 (48% [95% CI, 27% to 69%]) evaluable patients, and dose escalation could not be completed. Virologic markers and CD4 cell count did not significantly change. Conclusions: Hypericin caused significant phototoxicity and had no antiretroviral activity in the limited number of patients studied. C1 NYU, Sch Med, Med Ctr, New York, NY 10016 USA. Boston Univ, Sch Publ Hlth, Boston, MA 02215 USA. Beth Israel Hosp, AIDS Clin Trial Unit, Boston, MA 02215 USA. NIH, Rockville, MD USA. RP Gulick, RM (reprint author), Cornell Clin Trials Unit, Box 566,525 E 68th St, New York, NY 10011 USA. FU NCRR NIH HHS [M01-RR 0096]; NIAID NIH HHS [P30-AI 27742, U01-AI 27665] NR 20 TC 57 Z9 60 U1 0 U2 2 PU AMER COLL PHYSICIANS PI PHILADELPHIA PA INDEPENDENCE MALL WEST 6TH AND RACE ST, PHILADELPHIA, PA 19106-1572 USA SN 0003-4819 J9 ANN INTERN MED JI Ann. Intern. Med. PD MAR 16 PY 1999 VL 130 IS 6 BP 510 EP 514 PG 5 WC Medicine, General & Internal SC General & Internal Medicine GA 176RL UT WOS:000079165500007 PM 10075619 ER PT J AU Trinh, DP Brown, KM Jeang, KT AF Trinh, DP Brown, KM Jeang, KT TI Epithelin/granulin growth factors: Extracellular cofactors for HIV-1 and HIV-2 Tat proteins SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article DE HIV-1; HIV-2; Tat protein; protein binding; epithelin; granulin ID IMMUNODEFICIENCY-VIRUS TYPE-1; CARBOXYL-TERMINAL DOMAIN; RNA-POLYMERASE-II; CELLULAR PROTEIN; SPECIFICALLY INTERACTS; ACTIVATION DOMAIN; IN-VITRO; TRANSACTIVATOR; CELLS; IDENTIFICATION AB Epithelin/granulin growth factor is synthesized as a 593 amino acid precursor protein that contains 7.5 imperfectly conserved repeats of approximately 57 amino acids. Processed epithelin/granulin peptides have been isolated from vertebrate/invertebrate species and are growth factors implicated in epithelial and haemic cell function. Here they are identified as Human Immunodeficiency Virus (HIV) Tat binding proteins using the yeast two-hybrid assay. Intracellularly in yeast, mutation of selected cysteines in an epithelin/granulin dimeric repeat caused loss of binding to Tat exon 1. In vitro binding of HIV-1 and HIV-2 Tat to epithelin/granulin dimeric and monomeric repeats was also observed by GST-glutathione bead "pulldown" assays. Because Tat is actively secreted from HIV-infected cells and has been shown to serve as a mitogenic factor for angiogenesis and for Kaposi-like cells, our observations suggest that epithelin/granulin growth factors may function as biologically important extracellular Tat co-factors. (C) 1999 Academic Press. C1 George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA. NIAID, Mol Microbiol Lab, Mol Virol Sect, NIH, Bethesda, MD 20892 USA. RP Trinh, DP (reprint author), George Washington Univ, Dept Biol Sci, 332 Lisner Hall,2023 G St NW, Washington, DC 20052 USA. RI Jeang, Kuan-Teh/A-2424-2008 NR 35 TC 26 Z9 26 U1 0 U2 3 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 16 PY 1999 VL 256 IS 2 BP 299 EP 306 DI 10.1006/bbrc.1999.0317 PG 8 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 177YG UT WOS:000079238200009 PM 10079180 ER PT J AU Shastry, BS Hejtmancik, FJ Trese, MT AF Shastry, BS Hejtmancik, FJ Trese, MT TI Recurrent missense (R197C) and nonsense (Y89X) mutations in the XLRS1 gene in families with X-linked retinoschisis SO BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS LA English DT Article ID JUVENILE RETINOSCHISIS AB Congenital retinoschisis (RS) is a hereditary eye disorder characterized by intraretinal schisis and central and peripheral retinal lesion. The gene responsible for the X-linked retinoschisis (XLRS1) has recently been isolated and found to contain mutations in affected members of several families. In this communication, two families with X-linked RS were analyzed for possible disease-causing mutations by polymerase chain reaction amplification of exons followed by DNA sequencing. Our analyses reveal a missense mutation at codon 197 in exon 6 and a nonsense mutation in exon-4 of XLRS1 gene. These changes resulted in the replacement of a highly conserved arginine by a cysteine residue and introduced a premature termination signal at codon 89, respectively. These mutations, which are transmitted through three generations, cosegregated with the disease, and are not found in the unaffected family members and 150 normal X-chromosomes, are likely to be pathogenic in these families. (C) 1999 Academic Press. C1 Oakland Univ, Eye Res Inst, Rochester, MI 48309 USA. NEI, Bethesda, MD 20892 USA. William Beaumont Hosp, Dept Ophthalmol, Royal Oak, MI 48073 USA. RP Shastry, BS (reprint author), Oakland Univ, Eye Res Inst, Rochester, MI 48309 USA. FU NEI NIH HHS [EY05230] NR 12 TC 5 Z9 7 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0006-291X J9 BIOCHEM BIOPH RES CO JI Biochem. Biophys. Res. Commun. PD MAR 16 PY 1999 VL 256 IS 2 BP 317 EP 319 DI 10.1006/bbrc.1999.0323 PG 3 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 177YG UT WOS:000079238200012 PM 10079181 ER PT J AU Garland, CS Tarien, E Nirmala, R Clark, P Rifkind, J Eichhorn, GL AF Garland, CS Tarien, E Nirmala, R Clark, P Rifkind, J Eichhorn, GL TI Curvature of dinucleotide poised for formation of trinucleotide in transcription with Escherichia coli RNA polymerase SO BIOCHEMISTRY LA English DT Article ID DNA BUBBLE DUPLEXES; MOLECULAR-STRUCTURE; MAGNETIC-RESONANCE; ACTIVE-SITE; PROMOTER; CRYSTAL; BACTERIAL; ENHANCER; MODEL AB A frequently used schematic model of transcriptional elongation shows an RNA polymerase molecule moving along a linear DNA. This model is of course highly idealized and not compatible with promoter sequences [Gralla, J. D. (1991) Cell 66, 415-418; Schleif, R. (1992) Annu. Rev. Biochem. 61, 199-223] and regulatory proteins [Koleske, A. J., and Young, R. A. (1995) Trends Biochem. Sci. 20, 113-116; Dunaway, M., and Droge, P. (1989) Nature 341, 657-659; Muller, H. P., Sogo, J. M., and Schaffner, W. (1989) Cell 58, 767-777] located some distance away from the point of transcription initiation [Karsten, R., von Hippel, P. H., and Langowski, J. (1995) Trends Biochem. Sci. 20, 500-506]. These circumstances lead to the expectation of curvature along the DNA strand and require looping between sometimes distant points. We have now shown curvature in a dinucleotide formed at the very onset of transcription when it is poised for reaction with a mononucleotide to form a trinucleotide. The curvature became evident from the demonstration that a metal ion bound with a mononucleotide in the i+1 (elongation) site is approximately equidistant from bases at the 5' end (i-1 site) and 3' end (i site) of the dinucleotide. Similar results were obtained with three different dinucleotides and four mononucleotides. Curvature of the RNA initiate may reflect curvature of the DNA to which it is bound. These studies show curvature to be a significant feature in the interaction between DNA template and RNA elongate even at the very beginning of transcription. C1 NIA, Cellular & Mol Biol Lab, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. RP Garland, CS (reprint author), NIA, Cellular & Mol Biol Lab, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. NR 25 TC 2 Z9 2 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0006-2960 J9 BIOCHEMISTRY-US JI Biochemistry PD MAR 16 PY 1999 VL 38 IS 11 BP 3421 EP 3425 DI 10.1021/bi9820098 PG 5 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 182QX UT WOS:000079510600025 PM 10079088 ER PT J AU Lee-Huang, S Huang, PL Sun, YT Huang, PL Kung, HF Blithe, DL Chen, HC AF Lee-Huang, S Huang, PL Sun, YT Huang, PL Kung, HF Blithe, DL Chen, HC TI Lysozyme and RNases as anti-HIV components in beta-core preparations of human chorionic gonadotropin SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE AIDS; muramidase; urinary proteins; antiviral agents ID SARCOMA CELL-LINE; KAPOSIS-SARCOMA; RIBONUCLEASE; HORMONE; FRAGMENT; GROWTH; URINE; MICE AB Human chorionic gonadotropin (hCG) preparations contain activity against HIT: type 1 (HIV-1). However, there has been controversy about whether some biological activities of hCG beta-subunit (hCG beta) preparations are caused by the beta-subunit itself or other proteins present in the preparations, We report here the purification, characterization, and identification of three enzymes with anti-HIV activity present in the beta-core fraction of hCG beta prepared from the urine of pregnant women. The N-terminal amino acid sequence of one protein is identical to human urinary lysozyme C, and those of the other two are identical to human RNase A and urinary RNase U. We thus refer to these proteins as AVL (antiviral lysozyme) and AVR (antiviral RNases). In addition to HIV-1 inhibition, AVL is capable of lysing Micrococcus lysodeikticus. AVR digests a variety of RNA substrates, including RNA from HIV-1-infected cells. We also find that lysozyme from chicken egg white, human milk and human neutrophils and RNase A from bovine pancreas possess activity against HIV-I. These findings may offer additional strategies for the treatment of HIV-1 infection. C1 NYU, Sch Med, Dept Biochem, New York, NY 10016 USA. Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 USA. Harvard Univ, Sch Med, Boston, MA 02114 USA. Harvard Community Hlth Plan, Boston, MA 02114 USA. NCI, Frederick Canc Res & Dev Ctr, Lab Biochem Physiol, Frederick, MD 21701 USA. NICHHD, Contracept & Reprod Hlth Branch, NIH, Bethesda, MD 20892 USA. NICHHD, Endocrinol & Reprod Res Branch, NIH, Bethesda, MD 20892 USA. RP Chen, HC (reprint author), NYU, Sch Med, Dept Biochem, New York, NY 10016 USA. FU NIAID NIH HHS [AI-31343] NR 21 TC 215 Z9 226 U1 0 U2 9 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 2678 EP 2681 DI 10.1073/pnas.96.6.2678 PG 4 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500024 PM 10077570 ER PT J AU Sanz, P Moss, B AF Sanz, P Moss, B TI Identification of a transcription factor, encoded by two vaccinia virus early genes, that regulates the intermediate stage of viral gene expression SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID RNA-POLYMERASE; INITIATION-FACTOR; DNA-REPLICATION; CAPPING ENZYME; PURIFICATION; PROMOTERS; SEQUENCE; SUBUNIT; CORE AB Vaccinia virus early, intermediate, and late stage genes are sequentially transcribed by the viral RNA polymerase within the cytoplasm of infected cells. We found that the 34- and 45-kDa polypeptides encoded by vaccinia virus ORFs A8R and A23R, respectively, were necessary to reconstitute transcription of a template with an intermediate stage promoter. Coexpression of the A8R and A23R genes in Escherichia coli was required for in vitro activity. In addition, the two polypeptides copurified, indicating their association as protein subunits of a vaccinia virus intermediate transcription factor, This factor, which we named VITF-3, complemented three viral proteins-namely, the RNA polymerase. capping enzyme, and a 30-kDa protein called VITF-1 that is also a subunit of the RNA polymerase-and an unidentified cell factor called VITF-2, Expression of the A8R and A23R genes occurred between I and 5 h after vaccinia virus infection and was not prevented by an inhibitor of DNA replication, consistent with a role for VITF-3 in specifically regulating intermediate transcription in vivo. The vaccinia virus A8R and A23R genes are highly conserved among vertebrate poxviruses, but no other viral or cellular homologs were identified. C1 NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. RP Moss, B (reprint author), NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. EM bmoss@nih.gov NR 35 TC 24 Z9 26 U1 1 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 2692 EP 2697 DI 10.1073/pnas.96.6.2692 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500027 PM 10077573 ER PT J AU Sata, M Moss, J Vaughan, M AF Sata, M Moss, J Vaughan, M TI Structural basis for the inhibitory effect of brefeldin A on guanine nucleotide-exchange proteins for ADP-ribosylation factors SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PLECKSTRIN-HOMOLOGY DOMAINS; TRANS-GOLGI NETWORK; PHOSPHOLIPASE-D; SACCHAROMYCES-CEREVISIAE; ORGANELLE STRUCTURE; ARF PROTEINS; MEMBRANE; CELLS; BINDING; GTP AB Protein secretion through the endoplasmic reticulum and Golgi vesicular trafficking system is initiated by the binding of ADP-ribosylation factors (ARFs) to donor membranes, lending to recruitment of coatomer, bud formation, and eventual vesicle release. ARFs are approximate to 20-kDa GTPases that are active with bound GTP and inactive with GDP bound. Conversion of ARF-GDP to ARF-GTP is regulated by guanine nucleotide-exchange proteins. All known ARF guanine nucleotide exchange proteins contain a Sec7 domain of approximate to 200 amino acids that includes the active site and fall into two classes that differ in molecular size and susceptibility to inhibition by the fungal metabolite brefeldin A (BFA). To determine the structural basis of BFA sensitivity, chimeric molecules were constructed by using sequences from the Sec7 domains of BFA-sensitive yeast Sec7 protein (vSec7d) and the insensitive human cytohesin-1 (C-1Sec7). Biased on BFA inhibition of the activities of these molecules with recombinant yeast ARF2 as substrate, the Asp(965)-Met(975) sequence in ySec7d was shown to be responsible for BFA sensitivity. A C-1Sec7 mutant in which Ser(199), Asn(204), and Pro(209) were replaced with the corresponding ySec7d amino acids, Asp(965), Gln(970), and Met(975), exhibited BFA sensitivity similar to that of recombinant ySec7d (rySec7d). Single replacement in C-1Sec7 of Ser(199) or Pro(209) resulted in partial inhibition by BFA, whereas replacement of Gln(970) in ySec7d with Asn (as found in C-1Sec7) had no effect. ils predicted, the double C-1Sec7 mutant with S199D and k209M was BFA-sensitive, demonstrating that Asp(965) and Met(975) in ySec7d are major molecular determinants of BFA sensitivity. C1 NHLBI, Pulm Crit Care Med Branch, NIH, Bethesda, MD 20892 USA. RP Sata, M (reprint author), NHLBI, Pulm Crit Care Med Branch, NIH, Bldg 10,Room 5N-307,10 Ctr Dr MSC 1434, Bethesda, MD 20892 USA. NR 57 TC 50 Z9 51 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 2752 EP 2757 DI 10.1073/pnas.96.6.2752 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500037 PM 10077583 ER PT J AU Shin, SH Kogerman, P Lindstrom, E Toftgard, R Biesecker, LG AF Shin, SH Kogerman, P Lindstrom, E Toftgard, R Biesecker, LG TI GLI3 mutations in human disorders mimic Drosophila Cubitus interruptus protein functions and localization SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID PALLISTER-HALL SYNDROME; SONIC HEDGEHOG; GREIG-SYNDROME; TARGET; MOUSE AB Truncation mutations of the GL13 zinc finger transcription factor can cause Greig cephalopolysyndactyly syndrome (GCPS), Pallister-Hall syndrome (PHS), and postaxial polydactyly type A (PAP-A). GLI3 is homologous to Drosophila Cubitus interruptus (Ci), which regulates the patched (ptc), gooseberry (gsb), and decapentaplegic (dpp) genes. Ci is sequestered in the cytoplasm and is subject to posttranslational processing whereby the full-length transcriptional activator form (Ci(155)) can be cleaved to a repressor form (Ci(75)). Under hedgehog signaling, the Ci(155) form translocates to the nucleus whereas in the absence of hedgehog, the Ci(75) form translocates to the nucleus. Based on the correlation of GLI3 truncation mutations and the human phenotypes, He hypothesized that GLI3 shows transcriptional activation or repression activity and subcellular localization similar to Ci. Here we show that full-length GLI3 localizes to the cytoplasm and activates PTCH1 expression, which is similar to full-length Ci(155), PHS mutant protein (GLI3-PHS) localizes to the nucleus and represses GLI3-activated PTCH1 expression, which is similar to Ci(75). The GCPS mutant protein has no effect on GLI3-activated PTCH1 transcription, consistent with the role of haploinsufficiency in this disorder. The PAP-A mutant protein (GLD-P;SP-A) showed less specific subcellular localization but still inhibited GLI3-activated PTCH1 transcription, suggesting it may be a weaker allele than the GLI3-PHS mutation. These data show that GL13 mutations in humans mimic functional effects of the Drosophila ci gene and correlate with the distinct effects of these mutations on human development. C1 Natl Human Genome Res Inst, Genet Dis Res Branch, NIH, Bethesda, MD 20892 USA. Karolinska Inst, Dept Biosci, Huddinge, Sweden. RP Biesecker, LG (reprint author), Natl Human Genome Res Inst, Genet Dis Res Branch, NIH, Bldg 49,Room 4A80, Bethesda, MD 20892 USA. RI Kogerman, Priit/B-6333-2008 NR 22 TC 119 Z9 124 U1 0 U2 2 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 2880 EP 2884 DI 10.1073/pnas.96.6.2880 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500059 PM 10077605 ER PT J AU Drotschmann, K Clark, AB Tran, HT Resnick, MA Gordenin, DA Kunkel, TA AF Drotschmann, K Clark, AB Tran, HT Resnick, MA Gordenin, DA Kunkel, TA TI Mutator phenotypes of yeast strains heterozygous for mutations in the MSH2 gene SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE mismatch repair; microsatellite instability; frameshift mutation ID DNA MISMATCH REPAIR; SACCHAROMYCES-CEREVISIAE; MICROSATELLITE INSTABILITY; ESCHERICHIA-COLI; SOMATIC MUTATIONS; COLORECTAL-CANCER; BETA RECEPTOR; BINDING; PROTEINS; CELLS AB Heterozygosity for germ-line mutations in the DNA mismatch repair gene MSH2 predisposes humans to cancer. Here we use a highly sensitive reporter to describe a spontaneous mutator phenotype in diploid T-east cells; containing a deletion of only one MSH2 allele, We also identify fire MSH2 missense mutations that have dominant mutator effects in heterozygous cells when expressed at normal Levels from the natural MSH2 promoter. For example, a WO-fold mutator effect Is observed in an MSH2/msh2 diploid strain in which Gly(693). which is invariant in MutS homologs and involved in ATP hydrolysis, is changed to alanine DNA binding data suggest that mismatch repair is suppressed by binding of a mutant Msh2-Msh6 heterodimer to a mismatch with subsequent inability to dissociate from the mismatch in the presence of ATP. A dominant mutator effect also is observed in yeast when Gly(693) is changed to serine, An early onset colorectal tumor is heterozygous for the analogous Gly --> Ser mutation in hMSH2, and a second hMSH2 mutation ws not found, suggesting that this missense mutation may predispose to cancer,fa a dominant mutator effect. The mutator effects of the deletion mutant and the Gly --> Ala missense mutant in yeast MSH2 are enhanced by heterozygosity For a missense mutation in DNA polymerase delta that reduces its proofreading activity but is not a mutator in the heterozygous state. The synergistic effects of heterozygosity for mutations in two different genes that act in series to correct replication errors may be relevant to cancer predisposition. C1 NIEHS, Mol Genet Lab, Res Triangle Pk, NC 27709 USA. RP Kunkel, TA (reprint author), NIEHS, Mol Genet Lab, POB 12233, Res Triangle Pk, NC 27709 USA. OI Gordenin, Dmitry/0000-0002-8399-1836 NR 53 TC 67 Z9 69 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 2970 EP 2975 DI 10.1073/pnas.96.6.2970 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500075 PM 10077621 ER PT J AU Overwijk, WW Lee, DS Surman, DR Irvine, KR Touloukian, CE Chan, CC Carroll, MW Moss, B Rosenberg, SA Restifo, NP AF Overwijk, WW Lee, DS Surman, DR Irvine, KR Touloukian, CE Chan, CC Carroll, MW Moss, B Rosenberg, SA Restifo, NP TI Vaccination with a recombinant vaccinia virus encoding a "self" antigen induces autoimmune vitiligo and tumor cell destruction in mice: Requirement for CD4(+) T lymphocytes SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID DEFICIENT MICE; IN-VITRO; TYROSINASE; REJECTION; PROTEIN; PEPTIDE; IMMUNIZATION; TOLERANCE; MELANOMA; RECEPTOR AB Many human and mouse tumor antigens are normal, nonmutated tissue differentiation antigens, Consequently, immunization with these "self" antigens could induce autoimmunity. When we tried to induce immune responses to five mouse melanocyte differentiation antigens, gp100? MART-1, tyrosinase, and tyrosinase-related proteins (TRP) 1 and TRP-2, we observed striking depigmentation and melanocyte destruction only in the skin of mice inoculated with a vaccinia virus encoding mouse TRP-1, These mice rejected a lethal challenge of B16 melanoma, indicating the immune response against TRP-1 could destroy both normal and malignant melanocytes. Cytotoxic T lymphocytes specific for TRP-1 could not be detected in depigmented mice, but high titers of Ige anti-TRP-l antibodies were present, Experiments with knockout mice revealed an absolute dependence on major histocompatibility complex class II, but not major histocompatibility complex class I, for the induction of both vitiligo and tumor protection. Together, these results suggest that the deliberate induction of self-reactivity using a recombinant viral vector can lead to tumor destruction, and that in this model, CD4(+) T lymphocytes are an integral part of this process. Vaccine strategies targeting tissue differentiation antigens may be valuable in canters arising from nonessential cells and organs such as melanocytes, prostate, testis, breast, and ovary. C1 NCI, Surg Branch, Bethesda, MD 20892 USA. NEI, Immunol Lab, Bethesda, MD 20892 USA. NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. RP Restifo, NP (reprint author), NCI, Surg Branch, Bldg 10,Room 2B42, Bethesda, MD 20892 USA. RI Restifo, Nicholas/A-5713-2008; OI Restifo, Nicholas P./0000-0003-4229-4580 FU Intramural NIH HHS [Z01 BC010763-01, Z99 CA999999] NR 48 TC 305 Z9 315 U1 1 U2 13 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 2982 EP 2987 DI 10.1073/pnas.96.6.2982 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500077 PM 10077623 ER PT J AU DePaola, N Davies, PF Pritchard, WF Florez, L Harbeck, N Polacek, DC AF DePaola, N Davies, PF Pritchard, WF Florez, L Harbeck, N Polacek, DC TI Spatial and temporal regulation of gap junction connexin43 in vascular endothelial cells exposed to controlled disturbed flows in vitro SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE gene regulation; gap junctional cell communication; cell proliferation; shear stress ID SHEAR-STRESS; INTERCELLULAR COMMUNICATION; CORONARY-ARTERY; GENE-EXPRESSION; MESSENGER-RNA; ATHEROSCLEROSIS; LOCATION; WALL AB Hemodynamic regulation of the endothelial gap junction protein connexin43 (Cx43) was studied in a model of controlled disturbed flows in vitro. Cx43 mRNA, protein expression, and intercellular communication were mapped to spatial variations in fluid forces. Hemodynamic features of atherosclerotic lesion-prone regions of the, vasculature (flow separation and recirculation) were created for periods of 5, 16, and 30 h, with laminar shear stresses ranging between 0 and 13.5 dynes/cm(2). Within I h, endothelial Cx43 mRNA expression was increased in all cells when compared with no-flow controls, With highest levels (up to 6- to 8-fold) expressed in regions of flow recirculation corresponding to high shear stress gradients. At 16 hi Cx43 mRNA expression remained elevated in regions of flow disturbance, whereas in areas of fully developed, undisturbed laminar flaw? Cx43 expression returned to control levels. In all flow regions, typical punctate Cx43 immunofluorescence at cell borders was disrupted by 5 h, After 30 h of flow, disruption of gap junctions persisted in cells subjected to flow separation and recirculation, whereas regions of undisturbed flow were substantially restored to normal. These expression differences were reflected in sustained inhibition of intercellular communication (dye transfer) throughout the zone of disturbed flow (84.2 and 68.4%, inhibition at 5 and 30 h, respectively); in contrast, communication was fully reestablished by 30 h in cells exposed to undisturbed flow. Up-regulation of Cx43 transcripts, sustained disorganization of Cx43 protein, and impaired communication suggest that shear stress gradients in regions of disturbed flow regulate intercellular communication through the expression and function of Cx43. C1 Univ Penn, Inst Med & Engn, Philadelphia, PA 19104 USA. Rensselaer Polytech Inst, Dept Biomed Engn, Troy, NY 12180 USA. NIH, Lab Diagnost Radiol Res, Off Intramural Res, Bethesda, MD 20892 USA. RP Polacek, DC (reprint author), Univ Penn, Inst Med & Engn, 3340 Smith Walk,Room 1072, Philadelphia, PA 19104 USA. EM polacek@pobox.upenn.edu FU NHLBI NIH HHS [HL36049, R37 HL036049] NR 28 TC 175 Z9 177 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 3154 EP 3159 DI 10.1073/pnas.96.6.3154 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500107 PM 10077653 ER PT J AU Kaufman, S AF Kaufman, S TI A model of human phenylalanine metabolism in normal subjects and in phenylketonuric patients SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID AMINO-ACID; HYDROXYLASE-ACTIVITY; GENE; TYROSINE; L-PHENYLALANINE; CONVERSION; INVIVO; ADULTS AB The derivation of a quantitative model of phenylalanine metabolism in humans is described. The model is based on the kinetic properties of pure recombinant human phenylalanine hydroxylase and on estimates of the in vivo rates of phenylalanine transamination and protein degradation. Calculated values for the steady-state concentration of blood phenylalanine, rate of clearance of phenylalanine from the blood after an oral load of the amino acid, and dietary tolerance of phenylalanine all agree well with data from normal as well as from phenylketonuric patients and obligate heterozygotes. These calculated values may help in the decision about the degree of restriction of phenylalanine intake that is necessary to achieve a satisfactory clinical outcome in classical patients and in those with milder forms of the disease. C1 NIMH, Neurochem Lab, Bethesda, MD 20892 USA. RP Kaufman, S (reprint author), NIMH, Neurochem Lab, 36 Convent Dr,MSC 4096,Bldg 36,Room 3D30, Bethesda, MD 20892 USA. NR 46 TC 37 Z9 37 U1 0 U2 4 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 3160 EP 3164 DI 10.1073/pnas.96.6.3160 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500108 PM 10077654 ER PT J AU Thirion, S Troadec, JD Pivovarova, NB Pagnotta, S Andrews, SB Leapman, RD Nicaise, G AF Thirion, S Troadec, JD Pivovarova, NB Pagnotta, S Andrews, SB Leapman, RD Nicaise, G TI Stimulus-secretion coupling in neurohypophysial nerve endings: A role for intravesicular sodium? SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE exocytosis; neuropeptide secretion; calcium; vesicle swelling; x-ray microanalysis ID ELECTRON-PROBE MICROANALYSIS; CHROMAFFIN CELLS; VASOPRESSIN RELEASE; GRANULES INSITU; BEIGE MOUSE; CALCIUM; EXOCYTOSIS; VESICLES; COMPARTMENTS; PRODUCTS AB It is generally accepted that Ca is essentially involved in regulated secretion, but the role of this cation, as well as others such as Na, is not well understood. ho illustrative example occurs in neurohypophysial secretion, where an experimentally induced increase in the cytosolic concentration of Na+ can induce continuous neuropeptide release. In contrast, an increase in cytosolic Ca2+ will have only a transient stimulatory effect The secretion-promoting targets for Ca2+ are not known; they may be cytosolic, as is usually assumed, but they mag. also be intravesicular, especially in view of evidence that Ca-rich secretory vesicles are preferentially secreted. In the present work, we have investigated the movements of these cations into and out of secretory vesicles during stimulus-secretion coupling. Isolated rat neurohypophysial nerve endings were stimulated by potassium (55 mM) depolarization, and at 6 min (peak secretion) and 20 min after the onset of stimulation, the elemental content of individual secretory vesicles was measured by quantitative x-ray microanalysis. A depolarization-induced transient increase in intravesicular Na+ concentration was found to coincide with the onset of secretion, Moreover, only a predicted small fraction of peripheral vesicles-presumably the docked ones-vr-ere Nat-loaded. The low sulfur concentration of Na+-rich resides most likely resulted from vesicle swelling. The results suggest that high intravesicular Na+ concentrations in docked vesicles, occurring by Na+/Ca2+ exchange or by transient fusion pore opening, is a proximal event in exocytosis. C1 Univ Nice, Fac Sci, Ctr Commun Microscopie Appl, F-06108 Nice, France. Univ Nice, Fac Sci, Lab Physiol Cellulaire & Mol, F-06108 Nice, France. NINDS, Neurobiol Lab, Bethesda, MD 20892 USA. NIH, Bioengn & Phys Sci Program, Off Director, Bethesda, MD 20892 USA. RP Nicaise, G (reprint author), Univ Nice, Fac Sci, Ctr Commun Microscopie Appl, F-06108 Nice 02, France. NR 35 TC 12 Z9 12 U1 0 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 3206 EP 3210 DI 10.1073/pnas.96.6.3206 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500116 PM 10077662 ER PT J AU Adesanya, OO Zhou, J Samathanam, C Powell-Braxton, L Bondy, CA AF Adesanya, OO Zhou, J Samathanam, C Powell-Braxton, L Bondy, CA TI Insulin-like growth factor 1 is required for G(2) progression in the estradiol-induced mitotic cycle SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID MESSENGER-RIBONUCLEIC-ACID; SYSTEM GENE-EXPRESSION; FACTOR-I RECEPTOR; IGF-I; CELL-PROLIFERATION; NULL MUTATION; DNA-SYNTHESIS; RAT UTERUS; ESTROGEN; PROGESTERONE AB Insulin-like growth factor 1 (IGF1) has been proposed as a "G(1)-progression factor" and as a mediator of estradiol's (E2) mitogenic effects on the uterus. To test these hypotheses, we compared E2's mitogenic effects on the uteri of Igf1-targeted gene deletion (null) and wild-type littermate mice. The proportion of uterine cells involved in the cell cycle and G(1)- and S-phase kinetics were not significantly different in wild-type and Igf1-null mice. However, the appearance of E2-induced mitotic figures and cell number increases were profoundly retarded in Igf1-null uterine tissue. There was a significant increase in nuclear DNA concentration in Igf1-null cells, consistent with a G(2) arrest. Interestingly, apoptotic cells were also significantly reduced in abundance, and the normal massive apoptotic response to E2 withdrawal was absent in the Igf1-null uterus. These data show that Igf1 is an essential mediator of E2's mitogenic effects, with a critical role not in G(1) progression but in G(2) progression. C1 NICHHD, NIH, Dev Endocrinol Branch, Bethesda, MD 20892 USA. Genentech Inc, Dept Cardiovasc Res, S San Francisco, CA 94080 USA. RP Bondy, CA (reprint author), NICHHD, NIH, Dev Endocrinol Branch, Bldg 10,Room 10N262,10 Ctr Dr 1862, Bethesda, MD 20892 USA. NR 22 TC 97 Z9 100 U1 1 U2 1 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD MAR 16 PY 1999 VL 96 IS 6 BP 3287 EP 3291 DI 10.1073/pnas.96.6.3287 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 177RH UT WOS:000079224500130 PM 10077676 ER PT J AU Singh, JP Evans, JC Levy, D Larson, MG Freed, LA Fuller, DL Lehman, B Benjamin, EJ AF Singh, JP Evans, JC Levy, D Larson, MG Freed, LA Fuller, DL Lehman, B Benjamin, EJ TI Prevalence and clinical determinants of mitral, tricuspid, and aortic regurgitation (The Framingham Heart Study) SO AMERICAN JOURNAL OF CARDIOLOGY LA English DT Article ID STRUCTURALLY NORMAL HEARTS; VALVULAR REGURGITATION; COLOR-DOPPLER; ROOT SIZE; HYPERTENSION; ECHOCARDIOGRAPHY; DISEASE; OBESITY; AGE AB Little information is available on the prevalence and determinants of valvular regurgitation in the general population. This study sought to assess the prevalence and clinical determinants of mitral (MR), tricuspid (TR), and aortic (AR) regurgitation in a population-based cohort. Color Doppler echocardiography was performed in 1,696 men and 1,893 women (aged 54 +/- 10 years) attending a routine examination at the Framingham Study. After excluding technically poor echocardiograms, MR, TR, and AR were qualitatively graded from trace to severe. Multiple logistic regression analysis was used to examine the association of clinical variables with MR and TR (more than or equal to mild severity) and AR (more than or equal to trace severity). MR and TR of more than or equal to mild severity was seen In 19.0% and 14.8% of men and 19.1% and 18.4% of women, respectively, and AR of more than or equal to trace severity in 13.0% of men and 8.5% of women. The clinical determinants of MR were age (odds ratio [OR] 1.3/9.9 years, 95% confidence interval [CI] 1.2 to 1.5), hypertension (OR 1.6; 95% CI 1.2 to 2.0), and body mass index (OR 0.8/4.3 kg/m(2); 95% CI 0.7 to 0.9). The determinants of TR were age (OR 1.5/9.9 years; 95% CI 1.3 to 1.7), body mass index (OR 0.7/4.3 kg/m(2): 95% CI 0.6 to 0.8), and female gender (OR 1.2; 95% CI 1.0 to 1.6). The determinants of AR were age (OR 2.3/9.9 years; 95% CI 2.0 to 2.7) and male gender (OR 1.6; 95% CI 1.2 to 2.1). A substantial proportion of healthy men and women had detectable valvular regurgitation by color Doppler echocardiography. These data provide population-based estimates for comparison with patients taking anorectic drugs. (C) 1999 by Excerpta Medico, Inc. C1 NHLBI, Framingham Heart Study, Framingham, MA USA. NHLBI, Bethesda, MD 20892 USA. Boston Univ, Sch Med, Div Cardiol, Boston, MA 02118 USA. Boston Univ, Sch Med, Div Prevent Med, Boston, MA 02118 USA. Beth Israel Hosp, Div Cardiol, Boston, MA 02215 USA. Beth Israel Hosp, Div Clin Epidemiol, Boston, MA 02215 USA. Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Med, Boston, MA USA. RP Benjamin, EJ (reprint author), Boston Univ, Sch Med, Framingham Heart Study, 5 Thurber St, Framingham, MA 01702 USA. EM emelia@fram.nhlbi.nih.gov OI Benjamin, Emelia/0000-0003-4076-2336 FU NHLBI NIH HHS [N01-HC-38038]; NINDS NIH HHS [2-RO1-NS-17950-11] NR 27 TC 392 Z9 404 U1 0 U2 6 PU EXCERPTA MEDICA INC PI NEW YORK PA 245 WEST 17TH STREET, NEW YORK, NY 10011 USA SN 0002-9149 J9 AM J CARDIOL JI Am. J. Cardiol. PD MAR 15 PY 1999 VL 83 IS 6 BP 897 EP 902 DI 10.1016/S0002-9149(98)01064-9 PG 6 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 178AV UT WOS:000079244000015 PM 10190406 ER PT J AU Kitts, PA Green, G AF Kitts, PA Green, G TI An immunological assay for determination of baculovirus titers in 48 hours SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID NUCLEAR POLYHEDROSIS-VIRUS; GREEN FLUORESCENT PROTEIN; PLAQUE ASSAY; EXPRESSION; GENE; INFECTION; MARKER; VECTOR AB Th baculovirus expression system is a system of choice for expressing eukaryotic proteins. Large amounts of biologically active material can be generated using this system by infecting insect cells with a baculovirus expressing the target protein. At several stages during the production of a baculovirus stock, it is necessary to titer the virus. Current methods have long time lines and are either technically difficult or are limited to viruses expressing a reporter gene. The new assay described here yields titers in 48 h, is easy to perform using 96-well plates, and is applicable to any Autographa californica nucleopolyhedrovirus-based recombinant baculovirus. This assay uses an antibody to a viral envelope glycoprotein to detect infected cells via immunostaining. The titer is determined by counting foci of infection under a light microscope. The required incubation period is shortened considerably because infected cells express viral antigens long before the macroscopic signs of infection scored in other assays become apparent. Titers determined using this immunological assay are comparable, both in value and variability, to those obtained using a traditional method, provided that the stocks have titers above 10(4) pfu/ml. (C) 1999 Academic Press. C1 Clontech Labs Inc, Palo Alto, CA 94303 USA. RP Kitts, PA (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat, Bldg 38A,Rm 8N803,8600 Rockville Pike, Bethesda, MD 20894 USA. NR 20 TC 22 Z9 24 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD MAR 15 PY 1999 VL 268 IS 2 BP 173 EP 178 DI 10.1006/abio.1998.3042 PG 6 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 182PV UT WOS:000079508100002 PM 10075805 ER PT J AU Friedrich, K Wietek, S Lischke, A Wellbrock, C Kreitman, RJ Pastan, I Sebald, W AF Friedrich, K Wietek, S Lischke, A Wellbrock, C Kreitman, RJ Pastan, I Sebald, W TI A two-step selection approach for the identification of ligand-binding determinants in cytokine receptors SO ANALYTICAL BIOCHEMISTRY LA English DT Article ID HUMAN GROWTH-HORMONE; HUMAN GM-CSF; ERYTHROPOIETIN RECEPTOR; CRYSTAL-STRUCTURE; IL-5 RECEPTORS; BETA-SUBUNIT; GAMMA CHAIN; INTERLEUKIN-4; RESIDUES; ACTIVATION AB We have developed a novel cell-based method for the isolation and selection of mutant cytokine receptors with defects in ligand binding and applied it to the human interleukin-4 receptor. The experimental procedure is based upon the functional heterologous expression of receptor mutants in eukaryotic cells followed by a two-step selection procedure. Positive selection for cells that express receptor variants is achieved by means of an agonistic antibody that mediates cell survival through receptor dimerization. An IL-4-coupled toxin is subsequently used to select against cells expressing wild-type receptors. Cells expressing mutant receptors that are unable to bind the cytotoxic ligand survive and can be amplified. The procedure allows the isolation of rare receptor variants from cell pools containing predominantly wild-type cells. This method, which should be equally applicable to similar receptor systems, was used to demonstrate the importance of a critical charged amino acid residue in the human IL-4 receptor alpha-subunit for IL-4-induced receptor activation, (C) 1999 Academic Press. C1 Biozentrum, D-97074 Wurzburg, Germany. NCI, NIH, Bethesda, MD 20892 USA. RP Friedrich, K (reprint author), Biozentrum, D-97074 Wurzburg, Germany. EM khf@biozentrum.uni-wuerzburg.de NR 36 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-2697 J9 ANAL BIOCHEM JI Anal. Biochem. PD MAR 15 PY 1999 VL 268 IS 2 BP 179 EP 186 DI 10.1006/abio.1998.3078 PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA 182PV UT WOS:000079508100003 PM 10075806 ER PT J AU Gopalakrishna, R Gundimeda, U Anderson, WB Colburn, NH Slaga, TJ AF Gopalakrishna, R Gundimeda, U Anderson, WB Colburn, NH Slaga, TJ TI Tumor promoter benzoyl peroxide induces sulfhydryl oxidation in protein kinase C: Its reversibility is related to the cellular resistance to peroxide-induced cytotoxicity SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article; Proceedings Paper CT 89th Annual Meeting of the American-Association-for-Cancer-Research CY MAR 27-APR 01, 1998 CL NEW ORLEANS, LA SP Amer Assoc Canc Res DE benzoyl peroxide; protein kinase C; zinc fingers; oxidation of cysteine residues; tumor promoters ID MOUSE EPIDERMAL-CELLS; THIOL-SPECIFIC ANTIOXIDANT; ADP-RIBOSYLATION FACTOR; PHORBOL ESTER BINDING; IN-VITRO; TYROSINE PHOSPHORYLATION; SELENOCOMPOUNDS INDUCE; HYDROGEN-PEROXIDE; REGULATORY DOMAIN; PHOSPHOLIPASE-D AB Since tumor promoter benzoyl peroxide (BPO) mimics phorbol esters in some aspects, its effects on protein kinase C (PKC) were previously studied, However, in those studies due to the presence of thiol agents in the PHC preparations, the sensitive reaction of BPO with redox-active cysteine residues in PKC was not observed. In this study, by excluding thiol agents present in the purified PKC preparation, low concentrations of BPO modified PKC, resulting in the loss of both kinase activity and phorbol ester binding (IC50 = 0.2 to 0.5 mu M). This modification, which was not dependent on transition metals, was totally blocked by a variety of thiol agents including OSH, which directly reacted with BPO, Substoichiometric amounts of BPO (0.4 mol/mol of PKC) oxidized two sulfhydryls in PKC and inactivated the enzyme which was readily reversed by dithiothreitol, The regulatory domain having zinc thiolate structures supporting the membrane-inserting region provided the specificity for PKC reaction with BPO, which partitioned into the membrane. Unlike H2O2, BPO did not induce the generation of the Ca2+/lipid-independent activated form of PKC, Other redox-sensitive enzymes such as protein kinase A, phosphorylase kinase, and protein phosphatase 2A required nearly 25- to 100-fold higher concentrations of BPO for inactivation. EPO also inactivated PKC in a variety of cell types. In the JB6 (30 P-) nonpromotable cell Line and other normal cell lines, where BPO was more cytotoxic, it readily inactivated PKC due to a slow reversibility of this inactivation by the cell. However, in the JB6 (41 P+) promotable cell line, C3H10T1/2 and B16 melanoma cells, where BPO was less cytotoxic, it did not readily inactivate PKC due to a rapid reversibility of this inactivation by an endogenous mechanism. Nevertheless, BPO inactivated PKC at an equal rate in the homogenates prepared from all these cell types, Inclusion of NADPH reversed this inactivation in the homogenates to a different extent, presumably due to a difference in distribution of a protein disulfide reductase, which reverses this oxidative modification. BPO-induced modification of PKC occurred independent of the cellular status of GSH, However, externally added GSH and cell-impermeable thiol agents prevented the BPO-induced modification of PHC, Since BPO readily partitions into membranes, its reaction with redox-cycling thiols of membrane proteins such as PKC may trigger epigenetic events to prevent cytotoxicity, but favor tumor promotion. (C) 1999 Academic Press. C1 Univ So Calif, Sch Med, Dept Cell & Neurbiol, Los Angeles, CA 90033 USA. NCI, Cellular Oncol Lab, Bethesda, MD 20892 USA. NCI, Frederick Canc Res & Dev Ctr, Viral Carcinogenesis Lab, Frederick, MD 21702 USA. AMC Canc Res Ctr, Ctr Canc Causat & Prevent, Denver, CO 80214 USA. RP Univ So Calif, Sch Med, Dept Cell & Neurbiol, 1333 San Pablo St,MMR 330, Los Angeles, CA 90033 USA. EM rgopalak@hsc.usc.edu FU NCI NIH HHS [CA62146] NR 74 TC 14 Z9 14 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0003-9861 EI 1096-0384 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD MAR 15 PY 1999 VL 363 IS 2 BP 246 EP 258 DI 10.1006/abbi.1999.1100 PG 13 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 177UQ UT WOS:000079229800007 PM 10068446 ER PT J AU Yoshikawa, W Hara, H Takehara, T Shimonishi, M Sakai, H Shimizu, N Shimizu, S Wang, MH Hagiya, M Skeel, A Leonard, EJ AF Yoshikawa, W Hara, H Takehara, T Shimonishi, M Sakai, H Shimizu, N Shimizu, S Wang, MH Hagiya, M Skeel, A Leonard, EJ TI Characterization of free alpha- and beta-chains of recombinant macrophage-stimulating protein SO ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS LA English DT Article ID HEPATOCYTE GROWTH-FACTOR; TYROSINE KINASE; HUMAN-SERUM; RECEPTOR; IDENTIFICATION; EXPRESSION; MSP; GENE; MIGRATION; VARIANTS AB Human serum macrophage-stimulating protein (MSP) induces motile activity of murine resident peritoneal macrophages and is a growth and motility factor for epithelial cells. It belongs to the plasminogen-related family of kringle proteins, and is secreted as a single-chain, 78-kDa, biologically inactive pro-MSP. Proteolytic cleavage of pro-MSP at a single site yields active MSP, a disulfide-linked alpha beta-chain heterodimer, However cleavage of recombinant pro-RLSP yielded not only the disulfide-linked heterodimer, but also free alpha- and beta-chains, indicating that some of the recombinant molecules lacked an alpha beta-chain disulfide. We purified the free chains for characterization. The beta-chain of MSP has three extra cysteines, Cys(527), Cys(562), and Cys(672), which are not found in the plasminogen beta-chain. Disulfide bond analysis showed a Cys(527)-Cys(562), but also a Cys(588)-Cys(672). Coopting Cys(588) by Cys(627) prevented the expected formation of a disulfide between alpha-chain Cys(468) and beta-chain Cys(588). Concomitant studies determined structures of oligosaccharides at the three Asn-linked glycosylation sites of MSP, The oligosaccharides at the three Asn loci are heterogeneous; 11 different sugars were identified, all being sialylated fucosyl biantennary structures. We also located the pro-MSP signal peptide cleavage site at Gly(18)-Gln(19) and the scissile bond for formation of mature MSP at Arg(483)-Val(484). (C) 1999 Academic Press. C1 NCI, Frederick Canc Res & Dev Ctr, Immunopathol Sect, Frederick, MD 21702 USA. Toyobo Co Ltd, Ohtsu, Shiga 52002, Japan. RP Leonard, EJ (reprint author), NCI, Frederick Canc Res & Dev Ctr, Immunopathol Sect, Bldg 560,Rm 12-71, Frederick, MD 21702 USA. NR 27 TC 6 Z9 6 U1 0 U2 1 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0003-9861 J9 ARCH BIOCHEM BIOPHYS JI Arch. Biochem. Biophys. PD MAR 15 PY 1999 VL 363 IS 2 BP 356 EP 360 DI 10.1006/abbi.1998.1090 PG 5 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 177UQ UT WOS:000079229800020 PM 10068459 ER PT J AU St-Denis, JF Cabaniols, JP Cushman, SW Roche, PA AF St-Denis, JF Cabaniols, JP Cushman, SW Roche, PA TI SNAP-23 participates in SNARE complex assembly in rat adipose cells SO BIOCHEMICAL JOURNAL LA English DT Article DE GLUT4; insulin; syntaxin; vesicle-associated membrane protein (VAMP) ID INSULIN-STIMULATED TRANSLOCATION; SENSITIVE FUSION PROTEIN; MEMBRANE-FUSION; SUBCELLULAR-LOCALIZATION; CLOSTRIDIAL NEUROTOXINS; GLUCOSE TRANSPORTERS; GLUT4 TRANSLOCATION; VESICLE DOCKING; IN-VITRO; T-SNARE AB SNARE proteins are required for vesicle docking and fusion in eukaryotic cells in processes as diverse as homotypic membrane fusion and synaptic vesicle exocytosis [SNARE stands for SNAP receptor, where SNAP is soluble NSF attachment protein]. The SNARE proteins syntaxin 4 and vesicle-associated membrane protein (VAMP) 2/3 also participate in the insulin-stimulated translocation of GLUT4 from intracellular vesicles to the plasma membrane in adipose cells. We now report the molecular cloning and characterization of rat SNAP-23, a ubiquitously expressed homologue of the essential neuronal SNARE protein SNAP-25 (synaptosomal-associated protein of 25 kDa). Rat SNAP-23 is 86% and 98%, identical respectively to human and mouse SNAP-23. Southern blot analysis reveals that the rat, mouse and human SNAP-23 genes encode species-specific isoforms of the same protein. Co-immunoprecipitation of syntaxin 4 and SNAP23 shows association of these two proteins in rat adipose cell plasma membranes, and insulin stimulation does not alter the SNAP-23/syntaxin 4 complex. In addition, we demonstrate for the first time the participation of SNAP-23, along with syntaxin 4 and VAMP2/3, in the formation of 20 S SNARE complexes prepared using rat adipose cell membranes and recombinant cr-SNAP and NSF proteins. The stoichiometry of the SNARE complexes formed is essentially identical using membranes from either unstimulated or insulin-stimulated adipose cells. These data demonstrate that rat SNAP-23 associates with syntaxin 4 before insulin stimulation and is present in the SNARE complexes known to mediate the translocation of GLUT4 from intracellular vesicles to the plasma membrane of rat adipose cells. C1 NIH, NIDDK, Diabet Branch, Expt Diabet Metab & Nutr Sect, Bethesda, MD 20892 USA. NIH, Expt Immunol Branch, NCI, Bethesda, MD 20892 USA. RP Roche, PA (reprint author), Bldg 10,Rm 4B36, Bethesda, MD 20892 USA. EM paul.roche@nih.gov NR 40 TC 32 Z9 33 U1 0 U2 1 PU PORTLAND PRESS PI LONDON PA 59 PORTLAND PLACE, LONDON W1N 3AJ, ENGLAND SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD MAR 15 PY 1999 VL 338 BP 709 EP 715 DI 10.1042/0264-6021:3380709 PN 3 PG 7 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 179BX UT WOS:000079304600019 PM 10051443 ER PT J AU Curto, EV Kwong, C Hermersdorfer, H Glatt, H Santis, C Virador, V Hearing, VJ Dooley, TP AF Curto, EV Kwong, C Hermersdorfer, H Glatt, H Santis, C Virador, V Hearing, VJ Dooley, TP TI Inhibitors of mammalian melanocyte tyrosinase: In vitro comparisons of alkyl esters of gentisic acid with other putative inhibitors SO BIOCHEMICAL PHARMACOLOGY LA English DT Article DE melanogenesis; TRP-1; TRP-2; hydroquinone; arbutin; kojic acid; magnesium ascorbylphosphate; cosmeceutical; methyl gentisate; pigmentation ID MELANIN BIOSYNTHESIS; IN-VITRO; ACTIVATION; BINDING; ENZYME; CELLS AB To discover safe and effective topical skin-lightening agents, we have evaluated alkyl esters of the natural product gentisic acid (GA), which is related to our lead compound methyl gentisate (MG), and four putative tyrosinase inhibitors, utilizing mammalian melanocyte cell cultures and cell-free extracts. Desirable characteristics include the ability to inhibit melanogenesis in cells (IC50 < 100 mu g/mL) without cytotoxicity, preferably due to tyrosinase inhibition. Of the six esters synthesized, the smaller esters (e.g. methyl and ethyl) were more effective enzyme inhibitors (IC50 similar to 11 and 20 mu g/mL, respectively). For comparison, hydroquinone (HQ), a commercial skin "bleaching" agent, was a less effective enzyme inhibitor (IC50 similar to 72 mu g/mL), and was highly cytotoxic to melanocytes in vitro at concentrations substantially lower than the IC50 for enzymatic inhibition. Kojic acid was a potent inhibitor of the mammalian enzyme (IC50 similar to 6 mu g/mL), but did not reduce pigmentation in cells. Both arbutin and magnesium ascorbyl phosphate were ineffective in the cell-free and eel-based assays. MG at 100 mu g/mL exhibited a minimal inhibitory effect on DHICA oxidase (TRP-1) and no effect on DOPAchrome tautomerase (TRP 2), suggesting that MG inhibits melanogenesis primarily via tyrosinase inhibition. MG and GA were non-mutagenic at the hprt locus in V79 Chinese hamster cells, whereas Ha was highly mutagenic and cytotoxic. The properties of MG in vitro, including (1) pigmentation inhibition in melanocytes, (2) tyrosinase inhibition and selectivity, (3) reduced cytotoxicity relative to HQ, and (4) rack of mutagenic potential in mammalian cells, establish MG as a superior candidate skin-lightening agent. (C) 1999 Elsevier Science Inc. C1 So Res Inst, Birmingham, AL 35205 USA. German Inst Human Nutr, Potsdam, Germany. NCI, NIH, Bethesda, MD 20892 USA. RP Dooley, TP (reprint author), So Res Inst, 2000 9th Ave S, Birmingham, AL 35205 USA. OI Glatt, Hansruedi/0000-0001-6053-0562 NR 31 TC 159 Z9 169 U1 1 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0006-2952 J9 BIOCHEM PHARMACOL JI Biochem. Pharmacol. PD MAR 15 PY 1999 VL 57 IS 6 BP 663 EP 672 DI 10.1016/S0006-2952(98)00340-2 PG 10 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 166WT UT WOS:000078601400012 PM 10037452 ER PT J AU Bertolino, A Knable, MB Saunders, RC Callicott, JH Kolachana, B Mattay, VS Bachevalier, J Frank, JA Egan, M Weinberger, DR AF Bertolino, A Knable, MB Saunders, RC Callicott, JH Kolachana, B Mattay, VS Bachevalier, J Frank, JA Egan, M Weinberger, DR TI The relationship between dorsolateral prefrontal N-acetylaspartate measures and striatal dopamine activity in schizophrenia SO BIOLOGICAL PSYCHIATRY LA English DT Article DE N-acetylaspartate; dorsolateral prefrontal cortex; striatum; dopamine; radio-receptor imaging; microdialysis ID MAGNETIC-RESONANCE SPECTROSCOPY; POSITRON EMISSION TOMOGRAPHY; VENTRAL TEGMENTAL AREA; CEREBRAL BLOOD-FLOW; SUBCORTICAL DOPAMINE; ENDOGENOUS DOPAMINE; NUCLEUS-ACCUMBENS; CORTEX; BRAIN; LESIONS AB Background: Pathology of dorsolateral prefrontal cortex and dysregulation of dopaminergic neurons have been associated with the pathophysiology of schizophrenia, but how these phenomena relate to each other in patients has not been known. It has been hypothesized that prefrontal cortical pathology might induce both diminished steady-state and exaggerated responses of dopaminergic neurons to certain stimuli (e.g., stress). We examined the relationship between a measure of prefrontal neuronal pathology and striatal dopamine activity in patients with schizophrenia and in a nonhuman primate model of abnormal prefrontal cortical development. Methods: in the patients, we studied in vivo markers of cortical neuronal pathology with NMR spectroscopic imaging and of steady-state striatal dopamine activity with radioreceptor imaging, in the monkeys, we used the same NMR technique and in vivo microdialysis. Results: Measures of N-acetyl-aspartate concentrations (NAA) in dorsolateral prefrontal cortex strongly and selectively predicted D-2 receptor availability in the striatum (n = 14, rho = -.64, p < .01), suggesting that the greater the apparent dorsolateral prefrontal cortex pathology, the less the steady-state dopamine activity in these patients. A similar relationship between NAA measures in dorsolateral prefrontal cortex and steady-state dopamine concentrations in the striatum was found in the monkeys (n = 5, rho = .70, p < .05). We then tested in the same monkeys the relationship of prefrontal NAA and striatal dopamine overflow following amphetamine infusion into dorsolateral prefrontal cortex. Under these conditions, the relationship was inverted i.e., the greater the apparent dorsolateral prefrontal cortex pathology, the greater the dopamine release. Conclusions: These data demonstrate direct relationships between putative neuronal pathology in dorsolateral prefrontal cortex and striatal dopamine activity in human and nonhuman primates and implicate a mechanism for dopamine dysregulation in schizophrenia. (C) 1999 Society of Biological Psychiatry. C1 NIMH, Clin Brain Disorders Branch, Intramural Res Programs, NIH, Bethesda, MD 20892 USA. Univ Texas, Dept Neurobiol & Anat, Houston, TX USA. NIH, Lab Diagnost Radiol Res, OD, Bethesda, MD 20892 USA. RP Weinberger, DR (reprint author), NIMH, Clin Brain Disorders Branch, Intramural Res Programs, NIH, 10 Ctr Dr,Room 45235 MSC 1379, Bethesda, MD 20892 USA. RI Callicott, Joseph/C-9102-2009; Bertolino, Alessandro/O-6352-2016 OI Callicott, Joseph/0000-0003-1298-3334; Bertolino, Alessandro/0000-0002-1251-1380 NR 67 TC 83 Z9 84 U1 2 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0006-3223 J9 BIOL PSYCHIAT JI Biol. Psychiatry PD MAR 15 PY 1999 VL 45 IS 6 BP 660 EP 667 DI 10.1016/S0006-3223(98)00380-1 PG 8 WC Neurosciences; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 177YC UT WOS:000079237800002 PM 10187995 ER PT J AU Rabkin, CS Yang, Q Goedert, JJ Nguyen, G Mitsuya, H Sei, S AF Rabkin, CS Yang, Q Goedert, JJ Nguyen, G Mitsuya, H Sei, S TI Chemokine and chemokine receptor gene variants and risk of non-Hodgkin's lymphoma in human immunodeficiency virus-1-infected individuals SO BLOOD LA English DT Article ID CELL-DERIVED FACTOR-1; HIV-1 INFECTION; HOMOSEXUAL MEN; VIRUS; SDF-1; AIDS; ACTIVATION; CXCR4; CHEMOATTRACTANT; LESTR/FUSIN AB Normal B-lymphocyte maturation and proliferation are regulated by chemotactic cytokines (chemokines), and genetic polymorphisms in chemokines and chemokine receptors modify progression of human immunodeficiency virus-1 (HIV-1) infection. Therefore, 746 HIV-1-infected persons were examined for associations of previously described stromal cell-derived factor 1 (SDF-1) chemokine and CCR5 and CCR2 chemokine receptor gene variants with the risk of B-cell non-Hodgkin's lymphoma (NHL). The SDF1-3'A chemokine variant, which is carried by 37% of whites and 11% of blacks, was associated with approximate doubling of the NHL risk in heterozygotes and roughly a fourfold increase in homozygotes. After a median follow-up of 11.7 years, NHL developed in 6 (19%) of 30 SDF1-3'A/3'A homozygotes and 22 (10%) of 202 SDF1-+/3'A heterozygotes, compared with 24 (5%) of 514 wild-type subjects. The acquired immunodeficiency syndrome (AIDS)-protective chemokine receptor variant CCR5-Delta 32 was highly protective against NHL, whereas the AIDS-protective variant CCR2-641 had no significant effect, Racial differences in SDF1-3'A frequency may contribute to the lower risk of HIV-1-associated NHL in blacks compared with whites. SDF-1 genotyping of HIV-1-infected patients may identify subgroups warranting enhanced monitoring and targeted interventions to reduce the risk of NHL. This is a US government work. There are no restrictions on its use. C1 NCI, Viral Epidemiol Branch, Bethesda, MD 20892 USA. NCI, HIV & AIDS Malignancy Branch, Bethesda, MD 20892 USA. NCI, Expt Retrovirol Sect, Bethesda, MD 20892 USA. NCI, Sci Applicat Int Corp, HIV Clin Interface Lab, Frederick, MD 21701 USA. Kumamoto Univ, Sch Med, Dept Internal Med 2, Kumamoto 860, Japan. RP Rabkin, CS (reprint author), NCI, Viral Epidemiol Branch, MSC 7248, Bethesda, MD 20892 USA. FU NCI NIH HHS [N01-CP-40501, N01-CP-33002] NR 32 TC 70 Z9 71 U1 0 U2 0 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1200 19TH ST, NW, STE 300, WASHINGTON, DC 20036-2422 USA SN 0006-4971 J9 BLOOD JI Blood PD MAR 15 PY 1999 VL 93 IS 6 BP 1838 EP 1842 PG 5 WC Hematology SC Hematology GA 176RT UT WOS:000079166100008 PM 10068655 ER PT J AU Lozier, JN Metzger, ME Donahue, RE Morgan, RA AF Lozier, JN Metzger, ME Donahue, RE Morgan, RA TI Rhesus macaque as an animal model for hemophilia B gene therapy SO BLOOD LA English DT Article ID HUMAN FACTOR-IX; COAGULATION-FACTOR-IX; DEFICIENT MOUSE MODEL; EXPRESSION; MICE; VECTORS; SEQUENCE; DOGS; IDENTIFICATION; PROPHYLAXIS AB We have determined the 2905 nucleotide sequence of the rhesus macaque factor IX complementary DNA (cDNA) and found it to be greater than 95% identical to that of the human factor IX cDNA. The cDNA has a large 3' untranslated region like the human cDNA, but unlike the human cDNA has two polyadenylation sites 224 nucleotides apart that are used for transcription of the messenger RNA. The deduced amino acid sequence is greater than 97% identical to that of human factor IX, differing in only 11 of 461 amino acids in the complete precursor protein. We found a single silent polymorphism in the nucleotide sequence at the third position of the codon for asparagine at position 167 in the secreted protein (AAC/AAT). All residues subject to posttranslational modifications in the human protein are also found in the rhesus factor IX sequence. The high degree of homology between the rhesus and human factor IX proteins suggested the possibility that the human factor IX protein might be nonimmunogenic in the rhesus. We tested the immunogenicity of human factor IX in three rhesus macaques by repeated intravenous injections of monoclonal antibody-purified. plasma-derived human factor IX over the course of more than a year and assessed the recovery and half-life of the infused protein, as well as in vitro indicators of antihuman factor IX antibodies. Human factor IX recovery and half-life remained unchanged over the course of a year in the three animals studied, and aPTT mixing studies showed no evidence for neutralizing antihuman factor IX antibodies. An outbred, nonhuman primate model that permits assessment of the level and duration of factor IX expression as well as vector safety would complement the use of other (mouse and canine) hemophilia B animal models in current use for the development of gene therapy for hemophilia B. This is a US government work. There are no restrictions on its use. C1 NHLBI, Bethesda, MD 20892 USA. Natl Human Genome Res Inst, Bethesda, MD USA. RP Morgan, RA (reprint author), Bldg 10,Room 10C103,10 Ctr Dr, Bethesda, MD 20892 USA. NR 40 TC 30 Z9 30 U1 0 U2 0 PU AMER SOC HEMATOLOGY PI WASHINGTON PA 1200 19TH ST, NW, STE 300, WASHINGTON, DC 20036-2422 USA SN 0006-4971 J9 BLOOD JI Blood PD MAR 15 PY 1999 VL 93 IS 6 BP 1875 EP 1881 PG 7 WC Hematology SC Hematology GA 176RT UT WOS:000079166100013 PM 10068660 ER PT J AU Matsumoto, RR Bowen, WD de Costa, BR Houk, JC AF Matsumoto, RR Bowen, WD de Costa, BR Houk, JC TI Relationship between modulation of the cerebellorubrospinal system in the in vitro turtle brain and changes in motor behavior in rats: Effects of novel sigma ligands SO BRAIN RESEARCH BULLETIN LA English DT Article DE sigma receptors; red nucleus; turtle; dystonia ID GUINEA-PIG BRAIN; BINDING-SITES; H-3 (+)-PENTAZOCINE; RECEPTOR LIGANDS; AUTORADIOGRAPHIC LOCALIZATION; MEDIATED NEUROPROTECTION; ANTIPSYCHOTIC-DRUGS; PATTERN GENERATION; NEURONAL CULTURES; OPIATE RECEPTORS AB Saturation and competition binding studies showed that the turtle brain contains a sites labeled by both [H-3]di-o-tolylguanidine (DTG) and [H-3](+)-pentazocine. There was a significant correlation between the IC50 values of a ligands for [H-3]DTG sites in the turtle vs. rat brain, suggesting that the sites are comparable in the two species. In contrast, [H-3](+)-pentazocine, which primarily labels al sites in the rodent brain, labels a heterogeneity of sites in the turtle brain. In extracellular recordings from the in vitro turtle brainstem, some a ligands enhanced the burst responses of red nucleus (RN) neurons (DTG, haloperidol, BD1031, BD1052, BD1069) while other a ligands decreased the burst responses (BD1047, BD1063). Control compounds (turtle Ringer vehicle control, opiate antagonist naloxone, atypical neuroleptic sulpiride) had no significant effects on the RN burst responses recorded from the in vitro turtle brain. The ED(50)s of the ligands for altering the burst responses in RN neurons from the turtle brain were correlated with their IC(50)s for turtle brain sites labeled with [H-3]DTG, but not [H-3](+)-pentazocine; this pattern is identical to that previously reported in rats, where there is a correlation between the potencies of a ligands far producing dystonic postures after microinjection into the rat RN and their binding to rat brain sites labeled with [H-3]DTG, but not [H-3](+)-pentazocine. When the novel a ligands were microinjected into the rat RN, dystonic postures were produced by ligands that increased the burst duration of RN neurons in the turtle brain. Novel a ligands that reduced the burst responses in the in vitro turtle brain have previously been reported to have no effects on their own when microinjected into the rat RN, but to block the dystonic postures produced by other a ligands. Taken together, the data suggest that the opposite effects of the novel ligands in the turtle electrophysiological studies represent the actions of agonists vs. antagonists, and that the directionality of the effects has predictive value for the expected motor effects of the drugs. (C) 1999 Elsevier Science inc. C1 Univ Oklahoma, Hlth Sci Ctr, Coll Pharm, Dept Pharmacol & Toxicol, Oklahoma City, OK 73190 USA. Northwestern Univ, Sch Med, Dept Physiol, Chicago, IL 60611 USA. NIDDK, Med Chem Lab, NIH, Bethesda, MD 20892 USA. RP Matsumoto, RR (reprint author), Univ Oklahoma, Hlth Sci Ctr, Coll Pharm, Dept Pharmacol & Toxicol, POB 26901, Oklahoma City, OK 73190 USA. FU NIDA NIH HHS [DA05430]; NIMH NIH HHS [MH50564]; NINDS NIH HHS [NS21015] NR 89 TC 6 Z9 6 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0361-9230 J9 BRAIN RES BULL JI Brain Res. Bull. PD MAR 15 PY 1999 VL 48 IS 5 BP 497 EP 508 DI 10.1016/S0361-9230(99)00029-5 PG 12 WC Neurosciences SC Neurosciences & Neurology GA 199ND UT WOS:000080487800005 PM 10372510 ER PT J AU Ishikawa, Y Sugano, H Matsumoto, T Furuichi, Y Miller, RW Goto, M AF Ishikawa, Y Sugano, H Matsumoto, T Furuichi, Y Miller, RW Goto, M TI Unusual features of thyroid carcinomas in Japanese patients with Werner syndrome and possible genotype-phenotype relations to cell type and race SO CANCER LA English DT Article; Proceedings Paper CT 89th Annual Meeting of the American-Association-for-Cancer-Research CY MAR 27-APR 01, 1998 CL NEW ORLEANS, LOUISIANA SP Amer Assoc Canc Res DE Werner syndrome; genetic disease; cancer-prone disease; thyroid carcinoma; genotype-phenotype relation; racial differences ID SYNDROME GENE; MUTATIONS; DISEASE; FAMILY; CANCER AB BACKGROUND. Werner syndrome (WS), an autosomal recessive disease characterized by premature aging, has a high frequency of association with six rare neoplasms in Japanese patients, and only four of these neoplasms also occur excessively in whites. Several differ from what is usual in their epidemiology and/or histology. Described in this article are peculiarities in the occurrences of follicular and papillary thyroid carcinomas among Japanese patients and the possible genotype-phenotype relations pertaining to cell types and the absence of excess thyroid carcinoma occurrence in whites with WS. METHODS. Epidemiologic features of 23 histologically diagnosed thyroid carcinomas from a series of 150 cancers in 845 Japanese patients with WS were compared with those of 19,446 tumors in a Japanese national registry of thyroid carcinomas from 1977-1991. Germline mutations had been determined by molecular studies of peripheral blood. RESULTS. The average age of patients with thyroid carcinoma was 39 years for those with WS and 49 years for the registry patients. The female-to-male ratios were 2.3 : 1 and 6.6 : 1, respectively. The rates of occurrence-of papillary, follicular, and anaplastic carcinomas were 35%, 48%, and 13% for Japanese patients with WS and 78%, 14%, and 2% in the general Japanese population. AU four cases of follicular carcinoma had germline mutations of the WS gene in the C-terminal region, and the germline mutation for the only papillary carcinoma was in the N-terminal region. CONCLUSIONS. This study suggests two possible WS genotype-phenotype relations. One concerns thyroid carcinoma histology; the other concerns frequent mutations that occur in the C-terminal region in Japanese patients, but not in white patients, with WS. These may account for the excess thyroid carcinoma occurrence among Japanese. Cancer 1999;85:1345-52. (C) 1999 American Cancer Society. C1 Inst Canc, Dept Pathol, Toshima Ku, Tokyo 1708455, Japan. AGENE Res Inst, Kanagawa, Japan. NCI, Genet Epidemiol Branch, Bethesda, MD 20892 USA. Tokyo Metropolitan Otsuka Hosp, Tokyo, Japan. RP Ishikawa, Y (reprint author), Inst Canc, Dept Pathol, Toshima Ku, 1-37-1 Kami Ikebukuro, Tokyo 1708455, Japan. NR 44 TC 50 Z9 51 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0008-543X J9 CANCER JI Cancer PD MAR 15 PY 1999 VL 85 IS 6 BP 1345 EP 1352 DI 10.1002/(SICI)1097-0142(19990315)85:6<1345::AID-CNCR18>3.3.CO;2-R PG 8 WC Oncology SC Oncology GA 175KQ UT WOS:000079092900018 PM 10189141 ER PT J AU Murakami, M Gurski, KJ Marincola, FM Ackland, J Steller, MA AF Murakami, M Gurski, KJ Marincola, FM Ackland, J Steller, MA TI Induction of specific CD8+ T-lymphocyte responses using a human papillomavirus-16 E6/E7 fusion protein and autologous dendritic cells SO CANCER RESEARCH LA English DT Article ID IN-VITRO; CERVICAL-CANCER; ANTIGEN; PEPTIDES; TYPE-16; VIVO; E6 AB When intracellular viral proteins are degraded, only a limited number of peptide epitopes are capable of eliciting specific CD8(+) cellular immune responses for a given human leukocyte antigen (BLA) haplotype. We sought to induce CD8(+) T-lymphocyte (CTL) responses to human papillomavirus-16 (HPV-16) E6 and E7 proteins using a recombinant E6/E7 fusion protein and autologous human dendritic cells (DCs), CTLs were generated by bt vitro stimulation using a recombinant HPV-16 E6/E7 fusion protein and autologous DCs from a healthy HLA-A*0201 donor. CTL specificity was assessed by cytokine release assays when the cells were reacted with autologous DC targets coincubated with the E6/E7 fusion protein, These CTLs were also reacted with the immunodominant E7 peptides (E7(11-20) and E7(86-93)) and DCs as a target. As a negative control, DCs were incubated with or without an irrelevant control protein (Helicobacter pylori) as target for the E6/E7-induced CTLs. The E6/E7-induced CTLs were capable of specific recognition of target DCs coincubated with E6/E7 but not the control protein. When E6/E7-specific CTLs were reacted with DCs and either E7(11-20) or E7(86-93), specific peptide recognition was also detected, These data demonstrate that specific CTLs can be elicited using autologous human DCs and a HPV-16 E6/E7 fusion protein. Therefore, extracellular viral proteins seem to he engulfed and processed by DCs; then the immunodominant HLA-A2-restricted peptides become available fur CD8+ T-lymphocyte recognition. These data suggest that vaccine strategies using recombinant viral proteins may overcome the limitation of peptide epitopes for specific: HLA haplotypes and may, therefore, permit more generalized clinical application. C1 NCI, Gynecol Oncol Sect, Surg Branch, Div Clin Sci, Bethesda, MD 20892 USA. CSL Ltd, Parkville, Vic 3052, Australia. RP Steller, MA (reprint author), Brown Univ, Women & Infants Hosp, 1 Blackstone Pl,3rd Floor, Providence, RI 02905 USA. NR 22 TC 55 Z9 56 U1 1 U2 1 PU AMER ASSOC CANCER RESEARCH PI BIRMINGHAM PA PO BOX 11806, BIRMINGHAM, AL 35202 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 1999 VL 59 IS 6 BP 1184 EP 1187 PG 4 WC Oncology SC Oncology GA 175ZD UT WOS:000079125200004 PM 10096544 ER PT J AU Won, JW Kim, HT Park, EJ Hong, YC Kim, SJ Yun, YD AF Won, JW Kim, HT Park, EJ Hong, YC Kim, SJ Yun, YD TI Tumorigenicity of mouse thymoma is suppressed by soluble type II transforming growth factor beta receptor therapy SO CANCER RESEARCH LA English DT Article ID COLON-CARCINOMA CELLS; GASTRIC-CANCER CELLS; TGF-BETA; PROSTATE-CANCER; TUMOR-CELLS; T-CELLS; PROGRESSION; EXPRESSION; TGF-BETA-1; GENE AB Many types of tumor cells overexpress transforming growth factor beta (TGF-beta), which is believed to promote tumor progression. We hypothesized that overexpression of the extracellular region of the type II TGF-beta receptor (soluble T beta RII) would compete for or block TGF-beta binding to T beta Rs on immune cells, preventing TGF-beta-mediated immunosuppression and consequently resulting in the eradication of tumor cells, We tested this in the mouse thymoma cell line EL4, which has been reported to suppress cellular immunity by secreting a large amount of TGF-beta, Transduction of EL4 with recombinant retrovirus encoding soluble T beta RII resulted in the secretion of heterogeneously glycosylated, 25 to 35 kDa truncated T beta RII, Inoculation of 1 x 10(4) to 5 x 10(4) soluble T beta RII-modified EL4 cells (EL4/Ts, EL4 cells transduced with recombinant retrovirus encoding soluble T beta RII and neomycin resistance gene) s.c. to mice showed reduced tumorigenicity, as indicated by Lower overall tumor incidence (7%, 1 of 14; P < 0.001) compared with unmodified EL4 (100%, 9 of 9) or vector-modified EL4 cells (EL4/neo, EL4 cells transduced with recombinant retrovirus encoding neomycin resistance gene; 100%, 4 of 4), Administration of mitomycin C-treated EL4/Ts cells (1 x 10(6)) after EL4 inoculation (1 x 104) reduced tumor incidence from 100% (5 of 5 in mice inoculated with mitomycin C-treated EL4/neo) to 40% (4 of 10, P < 0.05), indicating that supply of soluble T beta RII could actually block TGF-beta-mediated tumorigenesis. In vitro tumor cytotoxicity assays revealed 3-5-fold higher cytotoxic activity with lymphocytes from EL4/Ts-bearing mice compared with those from EL4- or EL4/neo-bearing mice, indicating that the observed tumor rejection was mediated by restoration of the tumor-specific cellular immunity. These data suggest that expression of soluble T beta RII is an effective strategy for treating highly progressive tumors secreting TGF-beta. C1 Mogam Biotechnol Res Inst, Yongin City 449910, South Korea. NCI, Chemoprevent Lab, Bethesda, MD 20892 USA. RP Yun, YD (reprint author), Mogam Biotechnol Res Inst, 341 Pojung Ri, Yongin City 449910, South Korea. NR 40 TC 56 Z9 64 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI BIRMINGHAM PA PO BOX 11806, BIRMINGHAM, AL 35202 USA SN 0008-5472 J9 CANCER RES JI Cancer Res. PD MAR 15 PY 1999 VL 59 IS 6 BP 1273 EP 1277 PG 5 WC Oncology SC Oncology GA 175ZD UT WOS:000079125200019 PM 10096559 ER PT J AU Cacalano, NA Migone, TS Bazan, F Hanson, EP Chen, M Candotti, F O'Shea, JJ Johnston, JA AF Cacalano, NA Migone, TS Bazan, F Hanson, EP Chen, M Candotti, F O'Shea, JJ Johnston, JA TI Autosomal SCID caused by a point mutation in the N-terminus of Jak3: mapping of the Jak3-receptor interaction domain SO EMBO JOURNAL LA English DT Article DE common gamma chain; interleukin-2; Jak3; Jak kinase; receptor ID PROTEIN-TYROSINE KINASES; RECEPTOR GAMMA(C) CHAIN; CYTOKINE RECEPTORS; FAMILY; REGION; CELLS; GENE; IMMUNODEFICIENCY; PROLIFERATION; ASSOCIATION AB Signaling through the hematopoietic receptors requires activation of receptor-associated Janus (Jak) kinases, For example, Jak1 and Jak3 bind specifically to the IL-2 receptor beta (IL-2R beta) and common gamma (gamma(c)) chains, respectively, and initiate biochemical signals critical in controlling immune responses. The region of Jak responsible for receptor interactions, however, is not well characterized. Here we describe a naturally occurring Jak3 mutation from a patient with autosomal severe combined immunodeficiency (SCID), where a single amino acid substitution, Y100C, in Janus homology domain 7 (JH7) prevents kinase-receptor interaction. This mutation also results in a loss of IL-2-induced signaling in a B-cell line derived from this patient. Using mutational analysis we have identified a region of Jak3, including portions of JH6 and JH7, that is sufficient for kinase-receptor contact and show that this segment interacts with the proline-rich Box1 region of the receptor. Furthermore, a Jak3-Jak1 chimera containing only the JH6 and JH7 domains of Jak3 interacts with gamma(c) and can reconstitute IL-2-dependent responses, including receptor phosphorylation and activation of signal transducer and activator of transcription (STAT) 5b, Our results suggest that the N-terminus of Jak kinases is critical for receptor binding, and is therefore likely to determine specificity of Jak kinase-receptor interactions. C1 DNAX Res Inst Mol & Cellular Biol Inc, Res Inst, Palo Alto, CA 94304 USA. NIH, Clin Gene Therapy Branch, NHGRI, Bethesda, MD 20892 USA. NIAMSD, Lymphocyte Cell Biol Sect, Arthrit Rheumatism Branch, Bethesda, MD 20892 USA. RP Johnston, JA (reprint author), DNAX Res Inst Mol & Cellular Biol Inc, Res Inst, 901 Calif Ave, Palo Alto, CA 94304 USA. EM johnston@dnax.org RI Bazan, J. Fernando/B-4562-2010 NR 40 TC 79 Z9 84 U1 1 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0261-4189 J9 EMBO J JI Embo J. PD MAR 15 PY 1999 VL 18 IS 6 BP 1549 EP 1558 DI 10.1093/emboj/18.6.1549 PG 10 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 180KN UT WOS:000079385200012 PM 10075926 ER PT J AU Asano, K Krishnamoorthy, T Phan, L Pavitt, GD Hinnebusch, AG AF Asano, K Krishnamoorthy, T Phan, L Pavitt, GD Hinnebusch, AG TI Conserved bipartite motifs in yeast eIF5 and eIF2B epsilon, GTPase-activating and GDP-GTP exchange factors in translation initiation, mediate binding to their common substrate eIF2 SO EMBO JOURNAL LA English DT Article DE eIF2; evolution of eIFs; GAP; GEF; translation initiation complex ID COMPLETE GENOME SEQUENCE; FACTOR 4G EIF4G; SACCHAROMYCES-CEREVISIAE; PROTEIN-SYNTHESIS; SHUTTLE VECTORS; BETA-SUBUNIT; RNA-BINDING; START CODON; GCN4; IDENTIFICATION AB In the initiation phase of eukaryotic translation, eIF5 stimulates the hydrolysis of GTP bound to eIF2 in the 40S ribosomal pre-initiation complex, and the resultant GDP on eIF2 is replaced with GTP by the complex nucleotide exchange factor, eIF2B, Bipartite motifs rich in aromatic and acidic residues are conserved at the C-termini of eIF5 and the catalytic (epsilon) subunit of eIF2B. Here we show that these bipartite motifs are important for the binding of these factors, both in vitro and in vivo, to the beta subunit of their common substrate eIF2. We also find that three lysine-rich boxes in the N-terminal segment of eIF2 beta mediate the binding of eIF2 to both eIF5 and eIF2B. Thus, eIF5 and eIF2B epsilon employ the same sequence motif to facilitate interaction with the same segment of their common substrate. In agreement with this, archaea appear to lack eIF5, eIF2B and the lysine-rich binding domain for these factors in their eIF2 beta homolog, The eIF5 bipartite moth is also important for its interaction with the eIF3 complex through the NIP1-encoded subunit of eIF3, Thus, the bipartite motif in eIF5 appears to be multifunctional, stimulating its recruitment to the 40S preinitiation complex through interaction with eIF3 in addition to binding of its substrate eIF2. C1 NICHHD, Eukaryot Gene Regulat Lab, NIH, Bethesda, MD 20892 USA. Univ Dundee, Dept Anat & Physiol, Dundee DD1 5EH, Scotland. RP NICHHD, Eukaryot Gene Regulat Lab, NIH, Bethesda, MD 20892 USA. EM ahinnebusch@nih.gov RI Pavitt, Graham/A-1363-2010 OI Pavitt, Graham/0000-0002-8593-2418 NR 54 TC 152 Z9 159 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0261-4189 EI 1460-2075 J9 EMBO J JI Embo J. PD MAR 15 PY 1999 VL 18 IS 6 BP 1673 EP 1688 DI 10.1093/emboj/18.6.1673 PG 16 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA 180KN UT WOS:000079385200023 PM 10075937 ER PT J AU Ajmani, RS Fleg, JL Wright, JG Heim, JM Rifkind, JM AF Ajmani, RS Fleg, JL Wright, JG Heim, JM Rifkind, JM TI Treadmill exercise: Oxidative stress and hemorheology SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Labs Cellular & Mol Biol & Cardiovasc Sci, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1052 EP A1052 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902338 ER PT J AU Avichezer, D Silver, PB Chan, CC Caspi, RR AF Avichezer, D Silver, PB Chan, CC Caspi, RR TI Characterization of a new epitope of human IRBP which induces experimental autoimmune uveoretinitis (EAU) in H-2(b) mice. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, NEI, Immunol Lab, Bethesda, MD USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1000 EP A1000 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902044 ER PT J AU Braun, MC He, JH Wu, CY Kelsall, BL AF Braun, MC He, JH Wu, CY Kelsall, BL TI Cholera Toxin suppresses IL-12 production and IL-12 receptor beta 1 and beta 2 chain expression SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Immune Cell Interact Unit, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A648 EP A648 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900008 ER PT J AU Bruce, DS Newell, BJ Hanson, MA Oeltgen, PR Su, TP AF Bruce, DS Newell, BJ Hanson, MA Oeltgen, PR Su, TP TI Potency of an amino acid sequence from a "hibernation induction trigger" [HIT] 88 kDA fraction of hibernating woodchuck plasma SO FASEB JOURNAL LA English DT Meeting Abstract C1 Wheaton Coll, Dept Biol, Wheaton, IL 60187 USA. Univ Kentucky, Grad Ctr Toxicol, Lexington, KY 40511 USA. Natl Inst Drug Abuse, Neurosci Branch, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A741 EP A741 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900546 ER PT J AU Bukara, M Bautista, AP AF Bukara, M Bautista, AP TI Modulation of M-RNA expression for B-chemokines in hepatic sinusoidal endothelial cells (HSEC) during chronic alcohol intoxication. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Louisiana State Univ, Med Ctr, Dept Physiol, New Orleans, LA 70112 USA. Louisiana State Univ, Med Ctr, NIAAA, Sponsored Alcohol Res Ctr, New Orleans, LA 70112 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1131 EP A1131 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902792 ER PT J AU Caspi, RR Kang, Y Zambidis, E Scott, DW Chan, CC Agarwal, RK AF Caspi, RR Kang, Y Zambidis, E Scott, DW Chan, CC Agarwal, RK TI Gene therapy of experimental autoimmune uveitis (EAU). SO FASEB JOURNAL LA English DT Meeting Abstract C1 Amer Red Cross, Holland Lab, Dept Immunol, Rockville, MD 20855 USA. NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A992 EP A992 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901993 ER PT J AU Chambers-Slater, K Brams, P Black, A Padlan, EA Shestowsky, W Shearin, T Nguyen, ML Noelle, RJ Hanna, N Newman, R AF Chambers-Slater, K Brams, P Black, A Padlan, EA Shestowsky, W Shearin, T Nguyen, ML Noelle, RJ Hanna, N Newman, R TI A humanized anti-human CD154 monoclonal antibody blocks CD154-CD40 mediated human B cell activation SO FASEB JOURNAL LA English DT Meeting Abstract C1 Idec Pharmaceut Corp, San Diego, CA 92121 USA. NIDDK, Mol Biol Lab, NIH, Bethesda, MD 20892 USA. Dartmouth Med Sch, Dept Microbiol, Lebanon, NH 03756 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A988 EP A988 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901973 ER PT J AU Chen, M Cheng, A Candotti, F Zhou, YJ Hymel, A Notarangelo, LD O'Shea, JJ AF Chen, M Cheng, A Candotti, F Zhou, YJ Hymel, A Notarangelo, LD O'Shea, JJ TI Complex effects of naturally occurring mutations in the JAK3 pseudokinase domain: Evidence for interactions between the kinase and pseudokinase domains SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMS, ARB, Bethesda, MD 20892 USA. HHMI, Res Scholars Program, NIH, Bethesda, MD 20892 USA. NHGRI, CGB, NIH, Bethesda, MD 20892 USA. Univ Brescia, Brescia, Italy. RI Notarangelo, Luigi/F-9718-2016 OI Notarangelo, Luigi/0000-0002-8335-0262 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1145 EP A1145 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902875 ER PT J AU Cooper, CJ Hurst, SD Sitterding, SM Fuss, I Kansas, G Barrett, TA AF Cooper, CJ Hurst, SD Sitterding, SM Fuss, I Kansas, G Barrett, TA TI Antigen-driven trafficking of CD4(+) T cells to the intestine is inhibited with ANTI-IL-12 monoclonal antibody. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Northwestern Univ, Sch Med, Dept Microbiol Immunol, Chicago, IL 60611 USA. NIH, Bethesda, MD 20814 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A662 EP A662 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900096 ER PT J AU Dasso, JF Mage, RG Anderson, AO AF Dasso, JF Mage, RG Anderson, AO TI Comparative immunohistological development of the rabbit and human appendix. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. USA, Med Res Inst Infect Dis, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A968 EP A968 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901854 ER PT J AU Dmitrieva, N Kultz, D Burg, M AF Dmitrieva, N Kultz, D Burg, M TI High NaCl, but not urea increases p53 in renal inner medullary collecting duct cells (mIMCD). SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, Kidney & Electrolyte Metab Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A716 EP A716 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900400 ER PT J AU Dorfman, JR Stefanova, I Yasutomo, K Germain, RN AF Dorfman, JR Stefanova, I Yasutomo, K Germain, RN TI Signaling events result from the detection of self MHC by peripheral T cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunol Lab, NIH, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A950 EP A950 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901753 ER PT J AU Frederiksen, JK Martin, DA Zheng, L Siegel, RM Lenardo, MJ AF Frederiksen, JK Martin, DA Zheng, L Siegel, RM Lenardo, MJ TI Molecular mechanism of fas signaling defects in the human autoimmune lymphoproliferative syndrome SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. RI Frederiksen, John/C-4946-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1125 EP A1125 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902756 ER PT J AU Frucht, DM Aringer, M Galon, J Danning, C Boumpas, D O'Shea, J AF Frucht, DM Aringer, M Galon, J Danning, C Boumpas, D O'Shea, J TI STAT4 is expressed by activated peripheral blood monocytes, dendritic cells, and macrophages at sites of Th-1-mediated inflammation. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMS, NIH, Bethesda, MD 20814 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1151 EP A1151 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902908 ER PT J AU Gadina, M Sudarshan, C O'Shea, JJ AF Gadina, M Sudarshan, C O'Shea, JJ TI IL-2 but not IL-4 and other cytokines induces phosphorylation of a 98 kDa protein associated with SHP-2, phosphatidylinositol 3 '-kinase and Grb2 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMSD, Arthritis & Rheymatism Branch, Lymphocyte Cell Biol Sect, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1144 EP A1144 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902869 ER PT J AU Geiselhart, L Gregorio, T Humphries, C Komschlies, K AF Geiselhart, L Gregorio, T Humphries, C Komschlies, K TI Interleukin 7 (IL7) mediated signaling in T lymphocytes. SO FASEB JOURNAL LA English DT Meeting Abstract C1 SAIC, IRSP, Frederick, MD USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A948 EP A948 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901741 ER PT J AU Gelderman, MP Charukamnoetkanok, P Brady, JP Wawrousek, EF Zigler, JS Chan, CC Whitcup, SM Gery, I AF Gelderman, MP Charukamnoetkanok, P Brady, JP Wawrousek, EF Zigler, JS Chan, CC Whitcup, SM Gery, I TI Immune responses to deleted proteins in knockout (KO) mice: Implications for gene therapy. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1126 EP A1126 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902761 ER PT J AU Gitchell, H Wahlsten, J Chan, CC Wiggert, B Caspi, R AF Gitchell, H Wahlsten, J Chan, CC Wiggert, B Caspi, R TI B6.gld and B6.lpr mice are less susceptible than wild-type mice to induction of Experimental Autoimmune Uveoretinitis (EAU). SO FASEB JOURNAL LA English DT Meeting Abstract C1 NEI, Immunol Lab, NIH, Bethesda, MD 20892 USA. NEI, Lab Retinal & Mol Biol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1002 EP A1002 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902057 ER PT J AU Gonsky, R Deem, RL Young, HA Targan, SR AF Gonsky, R Deem, RL Young, HA Targan, SR TI Blood and guts: Peripheral blood and lamina propria T cells enlist different CIS elements for regulating transactivation of IFN-gamma expression SO FASEB JOURNAL LA English DT Meeting Abstract C1 Cedars Sinai Med Ctr, Ctr Inflammatory Bowel Dis, Los Angeles, CA 90048 USA. NCI, Frederick Canc Res & Dev Ctr, Expt Immunol Lab, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A647 EP A647 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900007 ER PT J AU Gubina, E Luo, X Sakamoto, K Shi, YF Mufson, RA AF Gubina, E Luo, X Sakamoto, K Shi, YF Mufson, RA TI Human interleukin-3 phosphorylates cAMP response element binding protein (CREB) and induces CRE/egr-1 promoter activity through protein kinase C (PKC) epsilon SO FASEB JOURNAL LA English DT Meeting Abstract C1 ARC, Holland Lab, Dept Immunol, Rockville, MD 20855 USA. Univ Calif Los Angeles, Sch Med, Div Hematol Oncol, Los Angeles, CA 90024 USA. NCI, Canc Immunol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1145 EP A1145 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902874 ER PT J AU Haddad, EK Roche, PA Henkart, PA AF Haddad, EK Roche, PA Henkart, PA TI A caspase-dependent increase in membrane recycling accompanies apoptotic death SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A981 EP A981 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901932 ER PT J AU Hahm, SH Eiden, LE AF Hahm, SH Eiden, LE TI A context-dependent binding of AP-1 is required for both PMA-inducible and cell-specific expression of the VIP gene. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, NIMH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A793 EP A793 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900836 ER PT J AU Hale-Donze, H Greenwell-Wild, T Doherty, TM Chatterjee, D Wahl, SM AF Hale-Donze, H Greenwell-Wild, T Doherty, TM Chatterjee, D Wahl, SM TI Mycobacterium avium complex (MAC) induction of macrophage-derived chemotactic factor(s). SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDCR, OIIB, Bethesda, MD 20892 USA. NIAID, LPD, NIH, Bethesda, MD 20892 USA. Colorado State Univ, Ft Collins, CO 80523 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A985 EP A985 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901952 ER PT J AU Han, M Harrison, L Kehn, P Stevenson, K Currier, J Robinson, MA AF Han, M Harrison, L Kehn, P Stevenson, K Currier, J Robinson, MA TI Human peripheral blood mononuclear cells contain multiple populaions of double negative (CD4-CD8-CD3+) T cells with conserved TCRA chains SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A942 EP A942 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901701 ER PT J AU Hannigan, MO Ai, Y Zhan, L Leto, T Huang, CK AF Hannigan, MO Ai, Y Zhan, L Leto, T Huang, CK TI Deficiency of p47 phox in neutrophils of db/db and db/? mice. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Connecticut, Ctr Hlth, Dept Pathol, Farmington, CT USA. NIAD, NIH, Host Def Lab, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A842 EP A842 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901119 ER PT J AU Hartt, J Barish, G Murphy, PM Gao, JL AF Hartt, J Barish, G Murphy, PM Gao, JL TI Molecular characterization of FPRL2R, a second mouse phagocyte chemotactic receptor specific for N-formylpeptides SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Host Def Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A659 EP A659 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900076 ER PT J AU Hesse, M Jankovic, D Cheever, AW Wynn, TA AF Hesse, M Jankovic, D Cheever, AW Wynn, TA TI A critical role for iNO-synthase and IL-10 in the regulation and development of type-1 cytokine responses induced by schistosome eggs and interleukin-12 SO FASEB JOURNAL LA English DT Meeting Abstract C1 LPD, NIH, Bethesda, MD 20892 USA. RI Wynn, Thomas/C-2797-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A972 EP A972 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901878 ER PT J AU Hochadel, JF Lewis, KC AF Hochadel, JF Lewis, KC TI Effects of administration of synthetic retinoids on vitamin A dynamics in a retinal pigment epithelial fell line (ARPE-19). SO FASEB JOURNAL LA English DT Meeting Abstract C1 SAIC, Intramural Res Support Program, Frederick, MD USA. NCI, Basic Res Lab, FCRDC, NIH, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A897 EP A897 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901441 ER PT J AU Hoshino, T Wiltrout, RH Young, AH AF Hoshino, T Wiltrout, RH Young, AH TI IL-18 is much more than an inducer of interferon-gamma: A role for IL-18 in IL-13 production SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, CMI, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, LEI, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, DBS, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A651 EP A651 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900031 ER PT J AU Hsich, E Johnson, T Zhou, YF Paigen, B Epstein, SE AF Hsich, E Johnson, T Zhou, YF Paigen, B Epstein, SE TI Cytomegalovirus (CMV) infection increases development of artherosclerosis in apo-E knockout mice. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Washington Hosp Ctr, Washington, DC 20010 USA. NHLBI, Bethesda, MD 20892 USA. NR 0 TC 4 Z9 4 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A692 EP A692 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900265 ER PT J AU Huang, H Niblack, E Shultz, L Paul, WE AF Huang, H Niblack, E Shultz, L Paul, WE TI Basal and IL-4 induced IL-4 receptor expression is controlled by PTPases. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAAA, LI, NIH, Bethesda, MD USA. Jackson Lab, Bar Harbor, ME USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1144 EP A1144 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902866 ER PT J AU Humphries, C Geiselhart, L Komschlies, K AF Humphries, C Geiselhart, L Komschlies, K TI IL7 pretreatment in vitro and in vivo enhances the proliferative response of CD8(+) T lymphocytes. SO FASEB JOURNAL LA English DT Meeting Abstract C1 SAIC, IRSP, Frederick, MD USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A949 EP A949 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901742 ER PT J AU Hwang, ST Saeki, H Yamada, N Brown, MJ Moore, AM AF Hwang, ST Saeki, H Yamada, N Brown, MJ Moore, AM TI Secondary Lymphoid-tissue Chemokine (SLC) and CCR7 participate in the emigration pathway of mature dendritic cells from the skin to regional lymph nodes SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Dermatol Branch, Bethesda, MD 20892 USA. NCI, Expt Immunol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A656 EP A656 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900059 ER PT J AU Jeon, US Joo, KW Ahn, C Han, JS Kim, S Lee, JS Kim, YH Kim, J Neilsen, S Knepper, MA AF Jeon, US Joo, KW Ahn, C Han, JS Kim, S Lee, JS Kim, YH Kim, J Neilsen, S Knepper, MA TI Redistribution and upregulation of AQP2 induced by oxytocin in rat kidney. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Catholic Univ, Coll Med, Seoul 110744, South Korea. Seoul Natl Univ, Coll Med, Seoul 110744, South Korea. Univ Aarhus, DK-8000 Aarhus, Denmark. NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A717 EP A717 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900406 ER PT J AU Jevremovic, D Billadeau, D Schoon, R Dick, C Irwin, B Zhang, W Samelson, L Abraham, R Leibson, P AF Jevremovic, D Billadeau, D Schoon, R Dick, C Irwin, B Zhang, W Samelson, L Abraham, R Leibson, P TI A role for the adaptor protein LAT in human NK cell-mediated cytotoxicity. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Mayo Clin, Rochester, MN 55905 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A961 EP A961 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901815 ER PT J AU Kallarakal, AT Rowland, AM Jacobowitz, DM AF Kallarakal, AT Rowland, AM Jacobowitz, DM TI Gene discovery in Parkinson's disease. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH, Natl Inst Hlth, Clin Sci Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1103 EP A1103 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902631 ER PT J AU Katusic, ZS Milstien, S Smith, L AF Katusic, ZS Milstien, S Smith, L TI Oxidized low density lipoprotein (oxLDL) decreases tetrahydrobiopterin (BH4) level in porcine coronary artery. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH, Bethesda, MD 20892 USA. Mayo Clin, Rochester, MN 55905 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A692 EP A692 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900264 ER PT J AU Katz, JL Alling, K AF Katz, JL Alling, K TI Discriminative stimulus effects of putative D3 dopamine receptor agonists in rats SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA, Intramural Res Program, Psychobiol Sect, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1105 EP A1105 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902643 ER PT J AU Khaled, AR Kim, K Reynolds, D Young, HA Youle, YR Muegge, K Durum, SK AF Khaled, AR Kim, K Reynolds, D Young, HA Youle, YR Muegge, K Durum, SK TI IL-7 induces transcription of bcl-2 and bcl-X-L and prevents mitochondrial translocation of Bax SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A981 EP A981 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901931 ER PT J AU Kobayashi, H Carrasquillo, JA Paik, CH Waldmann, TA Tagaya, Y AF Kobayashi, H Carrasquillo, JA Paik, CH Waldmann, TA Tagaya, Y TI Differences in pharmacokinetics and biodistribution. between interleukin-2 and interleukin-15. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. RI Carrasquillo, Jorge/E-7120-2010 NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1143 EP A1143 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902863 ER PT J AU Korthauer, U Nagel, W Davis, EM Le Beau, MM Menon, RS Mitchell, EO Kozak, CA Kolanus, W Bluestone, JA AF Korthauer, U Nagel, W Davis, EM Le Beau, MM Menon, RS Mitchell, EO Kozak, CA Kolanus, W Bluestone, JA TI Anergic T lymphocytes selectively express an integrin-regulatory protein of the cytohesin family SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Chicago, Ben May Inst Canc Res, Chicago, IL 60637 USA. Univ Chicago, Dept Med, Chicago, IL 60637 USA. Univ Munich, Mol Biol Lab, Gene Ctr, D-81377 Munich, Germany. NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A982 EP A982 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901938 ER PT J AU Korzick, DH Fishbein, KW Peterson, E Spencer, RSG Woodman, CR Laughlin, MH Lakatta, EG Sollott, SJ AF Korzick, DH Fishbein, KW Peterson, E Spencer, RSG Woodman, CR Laughlin, MH Lakatta, EG Sollott, SJ TI Perfusion-induced changes in myocardial contraction in the rat: Roles of nitric oxide and aging. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Missouri, Columbia, MO 65211 USA. NIA, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A782 EP A782 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900778 ER PT J AU Koshiba, M Rosin, DL Hayashi, N Linden, J Sitkovsky, MV AF Koshiba, M Rosin, DL Hayashi, N Linden, J Sitkovsky, MV TI Patterns of A(2A) extracellular adenosine receptor expression in different functional subsets of human peripheral T cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, NIH, Bethesda, MD 20892 USA. Kobe Univ, Sch Med, Kobe, Hyogo, Japan. Univ Virginia, Charlottesville, VA 22903 USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A944 EP A944 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901717 ER PT J AU Krausz, KW Yang, TJ Goldfarb, I Gonzalez, FJ AF Krausz, KW Yang, TJ Goldfarb, I Gonzalez, FJ TI Inhibitory monoclonal antibodies specific for human cytochrome P4502C8 and 2C9 define their roles in human liver microsomal metabolism SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Lab Mol Carcinogenesis, NIH, Bethesda, MD 20892 USA. NCI, Lab Metab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A811 EP A811 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900943 ER PT J AU Kurimjan, M Noben-Trauth, N Weis, JJ AF Kurimjan, M Noben-Trauth, N Weis, JJ TI IL-4 controls inflammation, not spirochete numbers, in Borrelia burgdorferi infection. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Utah, Sch Med, Salt Lake City, UT 84132 USA. NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A974 EP A974 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901892 ER PT J AU Lamensdorf, I Harvey-White, JD Hayakawa, Y Kopin, IJ AF Lamensdorf, I Harvey-White, JD Hayakawa, Y Kopin, IJ TI 3,4-dihydroxyphenylacetaldehyde(DOPAL) and 3,4-dihydroxyphenylethanol(DOPET) are formed rapidly in PC12 cells during metabolic stress. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, CNB, NIH, Bethesda, MD 20892 USA. NIDDK, LBC, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1037 EP A1037 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902254 ER PT J AU Lewis, KC Hochadel, JF AF Lewis, KC Hochadel, JF TI A mathematical modelling approach to assess retinoid chemopreventive and/or chemotherapeutic potential SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Basic Res Lab, NIH, Frederick, MD 21702 USA. FCRDC, SAIC, Intramural Res Support Program, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A867 EP A867 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901269 ER PT J AU Lindstrom, AL Xu, Z Schacker, T Clement-Burger, MJ Thurn, JR Smith, PD Wahl, SM Janoff, EN AF Lindstrom, AL Xu, Z Schacker, T Clement-Burger, MJ Thurn, JR Smith, PD Wahl, SM Janoff, EN TI Differential B cell function in mixed mononuclear cells and in purified populations in HIV-1-infected (HIV+) patients. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Minnesota, VAMC, Minneapolis, MN 55417 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A989 EP A989 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901980 ER PT J AU Liu, K Weng, NP AF Liu, K Weng, NP TI Molecular characterization of human CD4(+) naive and memory T cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, Immunol Lab, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A939 EP A939 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901684 ER PT J AU Manickan, E Satoi, J Vergella, J Liang, TJ AF Manickan, E Satoi, J Vergella, J Liang, TJ TI Liver specific regulation of transgenic expression by tetracycline in vivo. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, DDB NIDDK, Liver Dis Sect, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A678 EP A678 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900187 ER PT J AU Marshall, J Krump, E Thatcher, B Martin, B Curnutte, J Downey, G Grinstein, S Lindsay, T Walker, P Rubin, B AF Marshall, J Krump, E Thatcher, B Martin, B Curnutte, J Downey, G Grinstein, S Lindsay, T Walker, P Rubin, B TI HELSS covalently binds p(67) at three distinct sites. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Toronto, Toronto Hosp, Toronto, ON, Canada. Univ Toronto, Hosp Sick Children, Toronto, ON, Canada. Genentech Inc, San Francisco, CA USA. NIMH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1096 EP A1096 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902594 ER PT J AU Mayne, ST Risch, H Dubrow, R Chow, WH Blot, W Gammon, M Vaughan, T Farrow, DC Schoenberg, J Stanford, J Ahsan, H Fraumeni, JF AF Mayne, ST Risch, H Dubrow, R Chow, WH Blot, W Gammon, M Vaughan, T Farrow, DC Schoenberg, J Stanford, J Ahsan, H Fraumeni, JF TI Nutrient intake and risk of adenocarcinomas of the esophagus and gastric cardia. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Yale Univ, New Haven, CT 06520 USA. NCI, Bethesda, MD 20892 USA. Int Epidemiol Inst, Rockville, MD 20850 USA. Columbia Univ, New York, NY 10032 USA. Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1021 EP A1021 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902159 ER PT J AU McHugh, J AF McHugh, J TI Role of pertussis toxin-sensitive G-proteins in the regulation of calcium entry in human leukemia (HL-60) cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1034 EP A1034 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902235 ER PT J AU McHugh, RS Suri-Payer, E Shevach, EM AF McHugh, RS Suri-Payer, E Shevach, EM TI Induction of autoimmune gastritis (AIG) by autoantigen-specific Th2 cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1125 EP A1125 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902755 ER PT J AU McKinnon, JS Tucker, KL Masaki, K Resnick, H Foley, D Harris, T AF McKinnon, JS Tucker, KL Masaki, K Resnick, H Foley, D Harris, T TI Mid-life dietary carbohydrate associated with late-life depression. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. NIA, Bethesda, MD 20892 USA. RI Tucker, Katherine/A-4545-2010 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A937 EP A937 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901674 ER PT J AU Messam, CA Hou, J Major, EO AF Messam, CA Hou, J Major, EO TI Characterization of phenotypic markers in developing human brain cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, NIH, Lab Mol Med & Neurosci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A681 EP A681 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900204 ER PT J AU Miller, KA Witkin, JM Ungard, JT Gasior, M AF Miller, KA Witkin, JM Ungard, JT Gasior, M TI Pharmacological and behavioral characterization of cocaine-kindled seizures in mice. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA, Addict Res Ctr, Drug Dev Grp, Baltimore, MD 21224 USA. Duke Univ, Dept Pharmacol, Durham, NC 27706 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1107 EP A1107 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902656 ER PT J AU Mittelstadt, PR Ashwell, JD AF Mittelstadt, PR Ashwell, JD TI Role of Egr-2 in upregulation of Fas ligand SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Lab Immune Cell Biol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A979 EP A979 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901922 ER PT J AU Muegge, K Candeias, S Nakajima, H Leonard, WJ Baird, AM Berg, LJ Schlissel, M Durum, SK AF Muegge, K Candeias, S Nakajima, H Leonard, WJ Baird, AM Berg, LJ Schlissel, M Durum, SK TI IL-7 receptor control of TCR gamma gene rearrangement: Role of receptor-associatedchains and locus accessibility. SO FASEB JOURNAL LA English DT Meeting Abstract C1 SAIC Frederick, IRSP, Natl Canc Inst, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1145 EP A1145 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902872 ER PT J AU Nagel, JE Taub, DD AF Nagel, JE Taub, DD TI Effects of interferon-gamma-inducible protein (IP-10) and stromal cell-derived factor-1 (SDF-l alpha) on Jurkat cells by differential display. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, NIH, Immunol Lab, Gerontol Res Ctr, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A659 EP A659 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900073 ER PT J AU Noben-Trauth, N William, EP Sacks, DL AF Noben-Trauth, N William, EP Sacks, DL TI IFN gamma levels are not upregulated in BALB/c IL-4-/- or IL-4R alpha-/- mice infected with Leishmania major SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Natl Inst Hlth, Immunol Lab, Bethesda, MD 20892 USA. NIAID, Natl Inst Hlth, Parasitol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A647 EP A647 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900002 ER PT J AU Patterson, RM Stachlewitz, R Garofolo, M Germolec, DR AF Patterson, RM Stachlewitz, R Garofolo, M Germolec, DR TI Induction of apoptosis in TCDD-induced endotoxin hypersensitivity SO FASEB JOURNAL LA English DT Meeting Abstract C1 Natl Inst Environm Hlth Sci, Res Triangle Pk, NC USA. Inotek Corp, Cincinnati, OH 45219 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A976 EP A976 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901901 ER PT J AU Phung, QH Winter, DB Gearhart, PJ AF Phung, QH Winter, DB Gearhart, PJ TI Hypermutation in immunoglobulin variable genes from mice deficient in the MLH1 mismatch repair protein SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A992 EP A992 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901995 ER PT J AU Rabinovitz, M Bethesda, MD AF Rabinovitz, M Bethesda, MD TI Inhibition of phosphofructokinase by uncharged trna of amino acid deficiency : A unifying theory encompassing metabolic and cell cycle control. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A910 EP A910 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901521 ER PT J AU Rocha, G Peters, E Michea, L Ferguson, D Kirby, M Burg, M AF Rocha, G Peters, E Michea, L Ferguson, D Kirby, M Burg, M TI Non-steroidal analgesic drugs (NSAIDs) directly cause apoptosis in renal inner medullary collecting duct cells (mIMCD). SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, LKEM, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A722 EP A722 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900435 ER PT J AU Ruscetti, FW Mikovits, JA Baskar, PV Petrow, C Taub, DD AF Ruscetti, FW Mikovits, JA Baskar, PV Petrow, C Taub, DD TI Utilization of chemokine receptors on human mast cells by HIV-1: Biological consequences SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, IRSP, Lab Leuk Biol, Frederick, MD 21701 USA. NIA, Clin Immunol Sect, Immunol Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A975 EP A975 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901894 ER PT J AU Sada, K Zhang, J Siraganian, RP AF Sada, K Zhang, J Siraganian, RP TI Point mutation of a tyrosine in the linker region of Syk results in a gain of function. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. RI Sada, Kiyonao/H-7373-2015 OI Sada, Kiyonao/0000-0001-6124-3100 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A673 EP A673 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900155 ER PT J AU Schaeffer, EM Debnath, J McVicar, D Yap, G Sher, A Varmus, HE Lenardo, MJ Schwartzberg, PL AF Schaeffer, EM Debnath, J McVicar, D Yap, G Sher, A Varmus, HE Lenardo, MJ Schwartzberg, PL TI T cell defects in mice deficient in Rlk/Txk and Itk SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, HHMI NIH, NIAID, NCI,NHGRI,Res Scholars Program, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A946 EP A946 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901724 ER PT J AU Schaffer, E Key, M Taub, D AF Schaffer, E Key, M Taub, D TI Activation of opioid receptors on T cells results in the heterologous desensitization of chemokine-mediated responses. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, NIH, Immunol Lab, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A659 EP A659 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900074 ER PT J AU Schwab, C Starnes, J Lefkowitz, S Roberts, E Stuart, R Lefkowitz, D Gelderman, M Bollen, A Monguilevsky, N AF Schwab, C Starnes, J Lefkowitz, S Roberts, E Stuart, R Lefkowitz, D Gelderman, M Bollen, A Monguilevsky, N TI Induction of cytokine secretion by endothelial cells exposed to myeloperoxidase or other mannosylated compounds: Implication for inflammation SO FASEB JOURNAL LA English DT Meeting Abstract C1 Texas Tech Univ, Hlth Sci Ctr, Lubbock, TX 79430 USA. Texas Tech Univ, Lubbock, TX 79409 USA. NIH, Bethesda, MD 20892 USA. Free Univ Brussels, Brussels, Belgium. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1146 EP A1146 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902879 ER PT J AU Sehgal, D Schiaffella, E Anderson, OA Mage, RG AF Sehgal, D Schiaffella, E Anderson, OA Mage, RG TI Gene conversion and hypermutation during diversification of antibody sequences in rabbit splenic germinal centers. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, NIH, Bethesda, MD 20892 USA. USAMRIID, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A672 EP A672 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900150 ER PT J AU Shan, X Wange, RL AF Shan, X Wange, RL TI A requirement for ZAP-70 in the activation of Itk in Jurkat T cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, Biol Chem Lab, NIH, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A946 EP A946 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901727 ER PT J AU Silver, P Hathcock, K Chan, CC Wiggert, B Thompson, C Caspi, R AF Silver, P Hathcock, K Chan, CC Wiggert, B Thompson, C Caspi, R TI B7 blockade protects mice from a primary episode of Experimental Autoimmune Uveitis, but does not result in induction of long term tolerance SO FASEB JOURNAL LA English DT Meeting Abstract C1 NEI, Bethesda, MD 20892 USA. NCI, NIH, Bethesda, MD 20892 USA. Univ Chicago, Chicago, IL 60637 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1125 EP A1125 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902757 ER PT J AU Sinha, R Kulldorff, M Alavanja, MCR Swanson, CA AF Sinha, R Kulldorff, M Alavanja, MCR Swanson, CA TI Meat cooking, heterocyclic amines (HCAs) and risk of lung cancer. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. RI Kulldorff, Martin/H-4282-2011; Sinha, Rashmi/G-7446-2015 OI Sinha, Rashmi/0000-0002-2466-7462 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1021 EP A1021 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902162 ER PT J AU Song, XY Zeng, L Jin, WW Thompson, J Lei, KJ Billinghurst, CR Poole, AR Wahl, SM AF Song, XY Zeng, L Jin, WW Thompson, J Lei, KJ Billinghurst, CR Poole, AR Wahl, SM TI Secretory leukocyte protease inhibitor suppresses SCW-induced arthritis. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDCR, NIH, Bethesda, MD 20892 USA. Shriners Hosp Crippled Children, Montreal, PQ H3G 1A6, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1120 EP A1120 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902729 ER PT J AU Spong, CY Abebe, DT Gozes, I Brenneman, DE Hill, JM AF Spong, CY Abebe, DT Gozes, I Brenneman, DE Hill, JM TI Prevention of fetal alcohol syndrome by novel peptides SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHD, SDMP, NIH, Bethesda, MD 20892 USA. Tel Aviv Univ, IL-69978 Tel Aviv, Israel. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A881 EP A881 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901351 ER PT J AU Stolzenberg-Solomon, RZ Albanes, D Nieto, FJ Hartman, T Tangrea, J Rautalahti, M Sehlub, J Virtamo, J Taylor, P AF Stolzenberg-Solomon, RZ Albanes, D Nieto, FJ Hartman, T Tangrea, J Rautalahti, M Sehlub, J Virtamo, J Taylor, P TI Pancreatic cancer risk and nutrition-related methyl group availability indicators in male smokers SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. JHUSPH, Baltimore, MD 21205 USA. NPHI, Helsinki, Finland. Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. RI Albanes, Demetrius/B-9749-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A917 EP A917 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901561 ER PT J AU Sunday, ME Yoder, BA Cullen, A Cuttitta, F Emanuel, RL AF Sunday, ME Yoder, BA Cullen, A Cuttitta, F Emanuel, RL TI Neuropeptides in lung development and injury. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Harvard Univ, Sch Med, Boston, MA USA. SW Fdn Biomed Res, San Antonio, TX 78284 USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1154 EP A1154 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902924 ER PT J AU Sweet, DH Miller, DS Pritchard, JB AF Sweet, DH Miller, DS Pritchard, JB TI Subcellular localization of an organic anion transporter (rROAT1)/green fluorescent protein fusion construct. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS, Lab Pharmacol & Chem, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A717 EP A717 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900404 ER PT J AU Swift, ME Levin, J Kleinman, HK DiPietro, LA AF Swift, ME Levin, J Kleinman, HK DiPietro, LA TI Impaired wound repair and delayed angiogenesis in aged animals SO FASEB JOURNAL LA English DT Meeting Abstract C1 Loyola Univ, Dept Microbiol & Immunol, Maywood, IL 60153 USA. Loyola Univ, Dept Surg, Maywood, IL 60153 USA. NIDCR, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1006 EP A1006 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902076 ER PT J AU Taylor, LS McVicar, DW AF Taylor, LS McVicar, DW TI Functional association of Fc epsilon RI gamma with arginine(632) of paired immunoglobulin receptor (PIR)-A3 in murine macrophages. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Expt Immunol Lab, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1153 EP A1153 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902920 ER PT J AU Territo, PR Samuel, P Zhang, C Kennedy, SD Balaban, RS Wang, T AF Territo, PR Samuel, P Zhang, C Kennedy, SD Balaban, RS Wang, T TI Spectroscopic analysis of cardioplegically arrested rabbit hearts SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Cardiac Energet Lab, Bethesda, MD 20892 USA. Univ Rochester, Dept Surg, Rochester, NY 14642 USA. Univ Rochester, Dept Biophys, Rochester, NY 14642 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1079 EP A1079 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902494 ER PT J AU Territo, PR Mootha, VK French, SA Balaban, RS AF Territo, PR Mootha, VK French, SA Balaban, RS TI Calcium activation of cardiac oxidative phosphorylation: Direct evidence for activation of the F-0/F(1)ATPase. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1038 EP A1038 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902261 ER PT J AU Thornton, AM Shevach, EM AF Thornton, AM Shevach, EM TI Regulation of autoimmunity by CD4+CD25+T cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1127 EP A1127 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902771 ER PT J AU Tian, W Chua, K Strober, W Chu, CC AF Tian, W Chua, K Strober, W Chu, CC TI Isolation of genes expressed in B cells during class switching. SO FASEB JOURNAL LA English DT Meeting Abstract C1 N Shore Univ Hosp, Manhasset, NY 11030 USA. NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A996 EP A996 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902022 ER PT J AU Tomita, GM Wang, Y Paape, MJ Segal, DM Poutrel, B Rainard, P AF Tomita, GM Wang, Y Paape, MJ Segal, DM Poutrel, B Rainard, P TI Influence of bispecific antibodies on polymorphonuclear neutrophil function. SO FASEB JOURNAL LA English DT Meeting Abstract C1 USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. Univ Maryland, College Pk, MD 20742 USA. NIH, Bethesda, MD 20892 USA. INRA, F-37380 Nouzilly, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A841 EP A841 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901118 ER PT J AU Tong, ZB Nelson, LM AF Tong, ZB Nelson, LM TI A mouse gene encoding an ooplasm antigen in autoimmune premature ovarian failure. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHD, Dev Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1001 EP A1001 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902050 ER PT J AU Torday, JS Sunday, ME Londos, C Schultz, C Rubin, LP AF Torday, JS Sunday, ME Londos, C Schultz, C Rubin, LP TI Mechano-disregulation of coordinate growth factor-mediated cell-cell signalling pathways in bronchopulmonary dysplasia/chronic lung disease. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Calif Los Angeles, Los Angeles Cty Harbor Med Ctr, Dept Pediat, Torrance, CA 90502 USA. Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA. NIDDK, NIH, Bethesda, MD 20814 USA. Brown Univ, Dept Peds, Providence, RI 02912 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A820 EP A820 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900993 ER PT J AU Tranquill, LR Gran, B Zhou, W Dhib-Jalbut, S Martin, R AF Tranquill, LR Gran, B Zhou, W Dhib-Jalbut, S Martin, R TI Modulation of autoreactive, myelin basic protein-specific human T cell clones by copolymer 1 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Neuroimmunol Branch, Cellular Immunol Sect, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1004 EP A1004 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902065 ER PT J AU Turni, LA Shaw, S AF Turni, LA Shaw, S TI Development of a computer system for storing, retrieving and sharing diverse biological information SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A707 EP A707 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900348 ER PT J AU Vega, L Doherty, J Patterson, R Germolec, D AF Vega, L Doherty, J Patterson, R Germolec, D TI Arsenic-induced alterations in contact hypersensitivity SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS, Res Triangle Pk, NC 27709 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A663 EP A663 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900097 ER PT J AU Visser, M McQuillan, GM Bouter, LM Wener, MH Harris, TB AF Visser, M McQuillan, GM Bouter, LM Wener, MH Harris, TB TI Elevated inflammation status in obesity: NHANES III. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A925 EP A925 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901605 ER PT J AU Vistica, BP Matteson, D Chan, CC Wawrousek, E Lee, RS Whitcup, SM Gery, I AF Vistica, BP Matteson, D Chan, CC Wawrousek, E Lee, RS Whitcup, SM Gery, I TI Partial tolerance and immunopathology in double transgenic mice co-expressing hen egg lysozyme (HEL) in their lens and thymus and HEL-specific TCR by their T-cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1125 EP A1125 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902760 ER PT J AU Vogt, TM Ziegler, RG Swanson, CA Mayne, ST Hayes, RB AF Vogt, TM Ziegler, RG Swanson, CA Mayne, ST Hayes, RB TI Serum lycopene, beta-carotene, and risk of prostate cancer in US blacks and whites. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. Yale Univ, New Haven, CT 06510 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1021 EP A1021 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902160 ER PT J AU Weinstein, BM Hong, SK Huh, TL Pham, VN Fouquet, B Serluca, F Fishman, MC Roman, BL Bennett, PN AF Weinstein, BM Hong, SK Huh, TL Pham, VN Fouquet, B Serluca, F Fishman, MC Roman, BL Bennett, PN TI Dissecting trunk axial vessel formation using the zebrafish SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A694 EP A694 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900275 ER PT J AU Weinstein, S Ziegler, R Frongillo, E Fears, T Selhub, J AF Weinstein, S Ziegler, R Frongillo, E Fears, T Selhub, J TI Serum homocysteine levels and risk of cervical cancer SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. Cornell Univ, Ithaca, NY 14853 USA. Tufts Univ, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1021 EP A1021 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902163 ER PT J AU Welniak, LA Khaled, A Anver, M Reynolds, D Komschlies, K Wiltrout, R Young, H Blazar, BR Durum, S Murphy, WJ AF Welniak, LA Khaled, A Anver, M Reynolds, D Komschlies, K Wiltrout, R Young, H Blazar, BR Durum, S Murphy, WJ TI Non-immune action of interleukin-7 intestinal crypt cells of interleukin-7 receptor deficient mice are highly sensitive to gamma-radiation damage. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Minnesota, Minneapolis, MN 55455 USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A653 EP A653 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900040 ER PT J AU Weyer, C Snitker, S Rayussin, E AF Weyer, C Snitker, S Rayussin, E TI Energy metabolism in African-Americans: Potential risk factors for obesity. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDKD, Clin Diabet & Nutr Sect, NIH, Phoenix, AZ 85016 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A873 EP A873 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901304 ER PT J AU Whitney, LW Becker, KG Tresser, NJ McFarland, HF Biddison, WE Trent, JM AF Whitney, LW Becker, KG Tresser, NJ McFarland, HF Biddison, WE Trent, JM TI Differential gene expression in Multiple Sclerosis and experimental autoimmune encephalomyelitis (EAE) lesions identified using cDNA microarrays. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Neuroimmunol Branch, NIH, Bethesda, MD 20892 USA. NHGRI, Canc Genet Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1001 EP A1001 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902047 ER PT J AU Williams, MS Wu, ML Henkart, PA AF Williams, MS Wu, ML Henkart, PA TI Dependence of Fas ligand gene expression on TCR-stimulated generation of reactive oxygen species SO FASEB JOURNAL LA English DT Meeting Abstract C1 Amer Red Cross, Holland Lab, Dept Immunol, Rockville, MD 20855 USA. NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A980 EP A980 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901924 ER PT J AU Wood, RJ Fleet, JC Yergey, AL Vieira, N Bradley, J AF Wood, RJ Fleet, JC Yergey, AL Vieira, N Bradley, J TI Vitamin D receptor genotype and bone turnover in premenopausal women SO FASEB JOURNAL LA English DT Meeting Abstract C1 Tufts Univ, USDA, Human Nutr Res Ctr Aging, Bioavailabil Lab,JM, Boston, MA 02111 USA. NICHD, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A869 EP A869 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901279 ER PT J AU Wood, S Harris, T Lilly, M Norboo, T Eldridge, M AF Wood, S Harris, T Lilly, M Norboo, T Eldridge, M TI Effect of age, gender, and pregnancy on SaO2, Hb, and HR of Tibetan and Ladakhi highlanders SO FASEB JOURNAL LA English DT Meeting Abstract C1 Summa Hlth Syts, Akron, OH 44306 USA. NIEHS, Res Triangle Pk, NC 27709 USA. SNM Hosp, Leh 194101, Ladakh, India. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A785 EP A785 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900792 ER PT J AU Wu, CY Wang, KN Seder, RA AF Wu, CY Wang, KN Seder, RA TI Type 1 and type II interferons enhance IL-12 responsiveness in human CD4+T cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, NIH, Bethesda, MD 20802 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A944 EP A944 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901716 ER PT J AU Xu, H Wawrousek, EF Redmond, TM Nickerson, JM Wiggert, B Chan, CC Caspi, RR AF Xu, H Wawrousek, EF Redmond, TM Nickerson, JM Wiggert, B Chan, CC Caspi, RR TI Transgenic expression of IRBP induces a tolerance to the retinal antigen and reduces the susceptibility to experimental autoimmune uveoretinitis. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. Emory Univ, Atlanta, GA 30322 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A1000 EP A1000 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132902041 ER PT J AU Yamashiro, S Kamohara, H Yoshimura, T AF Yamashiro, S Kamohara, H Yoshimura, T TI Factors regulating delayed expression of monocyte chemoattractant protein-1 (MCP-1) in human neutrophils SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A658 EP A658 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900070 ER PT J AU Yang, T Huang, YG Briggs, JP Schnermann, JB AF Yang, T Huang, YG Briggs, JP Schnermann, JB TI Expression of cyclooxygenase-2 in collecting duct cells is regulated by nitric oxide. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A722 EP A722 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132900434 ER PT J AU Yin, DL Mufson, RA Wang, RX Shi, YF AF Yin, DL Mufson, RA Wang, RX Shi, YF TI Opioids induce expression of cell death receptor Fas SO FASEB JOURNAL LA English DT Meeting Abstract C1 Amer Red Cross, Holland Lab Biomed Sci, Dept Immunol, Rockville, MD 20855 USA. NCI, Can Immunol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 15 PY 1999 VL 13 IS 5 SU S BP A983 EP A983 PN 2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 228JG UT WOS:000082132901945 ER PT J AU Storz, G AF Storz, G TI An RNA thermometer SO GENES & DEVELOPMENT LA English DT Article ID HEAT-SHOCK RESPONSE; MESSENGER-RNA; ESCHERICHIA-COLI; TRANSLATION; PROTEIN; GENE C1 NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. RP Storz, G (reprint author), NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA. OI Storz, Gisela/0000-0001-6698-1241 NR 11 TC 24 Z9 24 U1 0 U2 7 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA SN 0890-9369 J9 GENE DEV JI Genes Dev. PD MAR 15 PY 1999 VL 13 IS 6 BP 633 EP 636 DI 10.1101/gad.13.6.633 PG 4 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA 181FB UT WOS:000079429500001 PM 10090718 ER PT J AU Tsukiyama, T Palmer, J Landel, CC Shiloach, J Wu, C AF Tsukiyama, T Palmer, J Landel, CC Shiloach, J Wu, C TI Characterization of the Imitation Switch subfamily of ATP-dependent chromatin-remodeling factors in Saccharomyces cerevisiae SO GENES & DEVELOPMENT LA English DT Article DE S-cerevisiae; ISW1; ISW2; chromatin-remodeling factors ID ASSEMBLY FACTOR-I; NUCLEOSOME DISRUPTION; TRANSCRIPTIONAL ACTIVATION; DROSOPHILA EMBRYOS; DNA-BINDING; YEAST; PROTEIN; FAMILY; GENE; RECOMBINATION AB We have identified and characterized two Imitation Switch genes in Saccharomyces cerevisiae ISW1 and ISW2, which we highly related to Drosophila ISWI, encoding the putative ATPase subunit of three ATP-dependent chromatin remodeling factors. Purification of ISW1p reveals a four-subunit complex with nucleosome-stimulated ATPase activity, as well as ATP-dependent nucleosome disruption and spacing activities, Purification of ISW2p reveals a two-subunit complex also with nucleosome-stimulated ATPase and ATP-dependent nucleosome spacing activities but no detectable nucleosome disruption activity. Null mutations of ISW1, ISW2, and CHD1 genes cause synthetic lethality in various stress conditions in yeast cells, revealing the first in vivo functions of the ISWI subfamily of chromatin-remodeling complexes and demonstrating their genetic interactions, A single point nutation within the ATPase domain of both ISW1p and ISW2p inactivated all ATP-dependent biochemical activities of the complexes, as well as the ability of the genes to rescue the mutant phenotypes. This demonstrates that the ATP-dependent chromatin-remodeling activities are essential for the in vivo functions of both ISW1 and ISW2 complexes. C1 NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA. NIDDK, Biotechnol Unit, NIH, Bethesda, MD 20892 USA. Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA. RP NCI, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA. EM ttsukiya@fhcrc.org NR 65 TC 261 Z9 267 U1 0 U2 6 PU COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT PI COLD SPRING HARBOR PA 1 BUNGTOWN RD, COLD SPRING HARBOR, NY 11724 USA SN 0890-9369 EI 1549-5477 J9 GENE DEV JI Genes Dev. PD MAR 15 PY 1999 VL 13 IS 6 BP 686 EP 697 DI 10.1101/gad.13.6.686 PG 12 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA 181FB UT WOS:000079429500008 PM 10090725 ER PT J AU Donze, D Adams, CR Rine, J Kamakaka, RT AF Donze, D Adams, CR Rine, J Kamakaka, RT TI The boundaries of the silenced HMR domain in Saccharomyces cerevisiae SO GENES & DEVELOPMENT LA English DT Article DE heterochromatin; silencing; HMR; boundary elements; S-cerevisiae ID MITOTIC CHROMOSOME CONDENSATION; TRANSCRIPTIONAL ACTIVATION; CHROMATIN STRUCTURE; PROTEIN; YEAST; DROSOPHILA; REPRESSION; GENES; RETROTRANSPOSONS; EXPRESSION AB The chromosomes of eukaryotes are organized into structurally and functionally discrete domains that provide a mechanism to compact the DNA as well as delineate independent units of gene activity. It is believed that insulator/boundary elements separate these domains, Here we report the identification and characterization of boundary elements that flank the transcriptionally repressed HMR locus in the yeast Saccharomyces cerevisiae. Deletion of these boundary elements led to the spread of silenced chromatin, whereas the ectopic insertion of these elements between a silencer and a promoter blocked the repressive effects of the silencer on that promoter at HMR and at telomeres. Sequence analysis indicated that the boundary element contained a TY1 LTR, and a tRNA gene and mutational analysis has implicated the Smc proteins, which encode structural components of chromosomes, in boundary element function. C1 NICHD, Unit Chromatin & Transcript, Bethesda, MD 20892 USA. Univ Calif Berkeley, Dept Mol & Cell Biol, Div Genet, Berkeley, CA 94720 USA. RP Kamakaka, RT (reprint author), NICHD, Unit Chromatin & Transcript, Bethesda, MD 20892 USA. FU NICHD NIH HHS [ZO1HD01904-01] NR 32 TC 278 Z9 281 U1 0 U2 2 PU COLD SPRING HARBOR LAB PRESS PI PLAINVIEW PA 1 BUNGTOWN RD, PLAINVIEW, NY 11724 USA SN 0890-9369 J9 GENE DEV JI Genes Dev. PD MAR 15 PY 1999 VL 13 IS 6 BP 698 EP 708 DI 10.1101/gad.13.6.698 PG 11 WC Cell Biology; Developmental Biology; Genetics & Heredity SC Cell Biology; Developmental Biology; Genetics & Heredity GA 181FB UT WOS:000079429500009 PM 10090726 ER PT J AU Kouprina, N Nikolaishvili, N Graves, J Koriabine, M Resnick, MA Larionov, V AF Kouprina, N Nikolaishvili, N Graves, J Koriabine, M Resnick, MA Larionov, V TI Integrity of human YACs during propagation in recombination-deficient yeast strains SO GENOMICS LA English DT Article ID ARTIFICIAL CHROMOSOME VECTORS; TRANSFORMATION-ASSOCIATED RECOMBINATION; CARRYING HUMAN DNA; SACCHAROMYCES-CEREVISIAE; MAMMALIAN-CELLS; GENE; CONSTRUCTION; RAD52; HOST; MANIPULATION AB Several isogenic strains with defects in recombination/repair genes (RAD1, RAD50, RAD51, RAD52, RAD54, and RAD55) were examined for their ability to propagate accurately a variety of linear and circular yeast artificial chromosomes (YACs) containing human DNA inserts. To assess YAC stability, the human DNA inserts were internally marked by an ADE2-pBR-URA3 cassette. Following selection for Ura(-) clones on 5-fluoroorotic acid containing medium, the following types of YAC deletions were identified: (i) those caused by homologous recombination with a telomeric pBR sequence; (ii) internal deletions, presumed to occur by recombination between commonly occurring DNA repeats such as Alu and LINE sequences; and (iii) deletions leading to loss of part of a YAC arm. rad52 host strains, but not other recombination-deficient strains, decreased the rate of all types of YAC deletions 25- to 400-fold. We have also developed and tested kar1 strains with a conditional RAD52 gene that allow transfer of a YAC from any host into a recombination-deficient background. These strains provide an efficient tool for stabilization of YACs and are useful for allowing additional recombinational modification of YACs. (C) 1999 Academic Press. C1 NIEHS, Mol Genet Lab, Res Triangle Pk, NC 27709 USA. RP Kouprina, N (reprint author), NIEHS, Mol Genet Lab, POB 12233, Res Triangle Pk, NC 27709 USA. EM Kouprina@niehs.nih.gov FU NHGRI NIH HHS [1-YO2-HG-60021-01] NR 51 TC 11 Z9 11 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 J9 GENOMICS JI Genomics PD MAR 15 PY 1999 VL 56 IS 3 BP 262 EP 273 DI 10.1006/geno.1998.5727 PG 12 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 180MP UT WOS:000079390000004 PM 10087193 ER PT J AU Taymans, SE Kirschner, LS Giatzakis, C Stratakis, CA AF Taymans, SE Kirschner, LS Giatzakis, C Stratakis, CA TI Radiation hybrid mapping of chromosomal region 2p15-p16: Integration of expressed and polymorphic sequences maps at the Carney complex (CNC) and Doyne honeycomb retinal dystrophy (DHRD) loci SO GENOMICS LA English DT Article ID STATISTICAL-METHODS; HUMAN GENOME; 2P; MUTATIONS; LINKAGE; CANCER; LOSSES; TUMORS AB Chromosomal region 2p15-p16, which corresponds to the genetic interval flanked by polymorphic markers D2S119 and D2S378 and covers a genetic distance of approximately 16 cM, is underrepresented in the existing maps of chromosome 2. This is primarily due to two large gaps of unknown physical distance within the known yeast and bacterial artificial chromosome (YAC and BAG, respectively) maps, In constructing a YAC/BAC contig covering 2p15-p16, a total of 55 sequence-tagged sites (25 of which are polymorphic), including new sequences derived from chromosomal walking, and 38 expressed sequence tags were screened by a commercially available RH panel (Stanford G3). A total of 45 of these sequences were placed; 32 of them were assigned at unique sites, The high-resolution TNG3 RH panel was then used to define further the chromosomal order of markers contained in the region flanked by D2S391 and D2S2153, This region harbors the genes for two autosomal dominant disorders, Carney complex (CNC), a multiple neoplasia syndrome, and Doyne honeycomb retinal dystrophy (DHRD), a disease leading to blindness at a young age. This is the first attempt to order cloned sequences in chromosomal region 2p15-p16, an area apparently resistant to YAC cloning, Construction of the 2p15-p16 RH map is critical for identifying the genes responsible for CNC and DHRD, as well as for the molecular elucidation of a chromosomal region that is frequently rearranged in tumors, (C) 1999 Academic Press. C1 NIH, NICHD, DEB, SPE,Unit Genet & Endocrinol, Bethesda, MD 20892 USA. RP Taymans, SE (reprint author), NIH, NICHD, DEB, SPE,Unit Genet & Endocrinol, Bldg 10,Room 10N262,10 Ctr Dr,MSC1862, Bethesda, MD 20892 USA. NR 32 TC 15 Z9 16 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 J9 GENOMICS JI Genomics PD MAR 15 PY 1999 VL 56 IS 3 BP 344 EP 349 DI 10.1006/geno.1998.5720 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 180MP UT WOS:000079390000014 PM 10087203 ER PT J AU Hunt, CR Parsian, AJ Kozak, CA AF Hunt, CR Parsian, AJ Kozak, CA TI Genetic mapping of mouse heat shock protein genes Hsc4a to chromosome 11 and Hsc74 to chromosome 18 and two Hsc74 pseudogenes to chromosomes X and 8 SO GENOMICS LA English DT Article ID HSP70 C1 Washington Univ, Sch Med, St Louis, MO 63108 USA. NIH, NIAID, Mol Microbiol Lab, Bethesda, MD 20892 USA. RP Hunt, CR (reprint author), Washington Univ, Sch Med, 4511 Forest Pk Blvd, St Louis, MO 63108 USA. FU NCI NIH HHS [CA 60757] NR 4 TC 0 Z9 0 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 J9 GENOMICS JI Genomics PD MAR 15 PY 1999 VL 56 IS 3 BP 358 EP 360 DI 10.1006/geno.1998.5716 PG 3 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 180MP UT WOS:000079390000019 PM 10087208 ER PT J AU Luostarinen, T af Geijersstam, V Bjorge, T Eklund, C Hakama, M Hakulinen, T Jellum, E Koskela, P Paavonen, J Pukkala, E Schiller, JT Thoresen, S Youngman, LD Dillner, J Lehtinen, M AF Luostarinen, T af Geijersstam, V Bjorge, T Eklund, C Hakama, M Hakulinen, T Jellum, E Koskela, P Paavonen, J Pukkala, E Schiller, JT Thoresen, S Youngman, LD Dillner, J Lehtinen, M TI No excess risk of cervical carcinoma among women seropositive for both HPV16 and HPV6/11 SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID HUMAN PAPILLOMAVIRUS TYPE-16; VIRUS-LIKE PARTICLES; CANCER; ANTIBODIES; INFECTION; SMOKING; COHORT AB Human papillomavirus (HPV) types 16 and 18 are the major risk factors for cervical carcinoma, whereas HPV types 6 and 11 cause benign genital lesions. We wanted to study the joint effect of simultaneous infections with the oncogenic and non-oncogenic HPV types on risk of subsequent development of cervical carcinoma. A cohort of 530,000 women who had donated blood samples to Nordic serum banks between 1973 and 1994 was followed up by linkage to national cancer registries. We identified 182 prospective cases with invasive cervical carcinoma and selected 538 matched controls at random. HPV 6, 11, 16, 18 and 33 seropositivity was used as a marker for the different HPV infections, and seropositivity for Chlamydia trachomatis and cotinine were used as markers for risk-taking sexual behavior and smoking respectively. The adjusted odds ratio (OR) of cervical squamous-cell carcinoma (SCC) was 2.2 for HPV6/11 among HPV 16 seronegatives and 5.5 for HPV16 among HPV6/11 seronegatives. Assuming multiplicative joint effect, the expected OR for seropositivity to both HPV6/11 and HPV16 would have been 12, but the observed OR was 1.0. The antagonistic interaction was statistically significant (P = 0.001) and present also under deterministic considerations of possible misclassification bias, Antagonistic interactions were also detected for combinations of HPV16 and HPV18 and of HPVI6 and HPV33, The results are in line with the concept that HPV-specific immunity protects against SCC and support primary prevention of SCC by vaccination against the HPVs. Int J. Cancer 80:818-822, 1999. (C) 1999 Wiley-Liss, Inc. C1 Finnish Canc Registry, Inst Stat & Epidemiol Canc Res, Helsinki 00171, Finland. Karolinska Inst, Ctr Microbiol & Tumor Biol, Stockholm, Sweden. Canc Registry Norway, Inst Epidemiol Canc Res, Oslo, Norway. Norwegian Radium Hosp, Dept Gynecol Oncol, Oslo, Norway. Univ Tampere, Tampere Sch Publ Hlth, FIN-33101 Tampere, Finland. Deutsch Krebsforschungszentrum, Div Epidemiol, D-6900 Heidelberg, Germany. Karolinska Univ Hosp, Radiumhemmet, Dept Canc Epidemiol, Stockholm, Sweden. Univ Helsinki, Dept Publ Hlth, Helsinki, Finland. Univ Oslo, Rikshosp, Inst Clin Biochem, N-0027 Oslo, Norway. Natl Publ Hlth Inst, Dept Oulu, Oulu, Finland. Univ Helsinki, Dept Obstet & Gynecol, Helsinki, Finland. Natl Canc Inst, Cellular Oncol Lab, Bethesda, MD USA. Univ Oxford, Clin Trial Serv Unit, Oxford, England. Umea Univ, Dept Nutr Res & Pathol, Umea, Sweden. Natl Publ Hlth Inst, Dept Infect Dis Epidemiol, Helsinki, Finland. RP Luostarinen, T (reprint author), Finnish Canc Registry, Inst Stat & Epidemiol Canc Res, Box 169, Helsinki 00171, Finland. NR 23 TC 54 Z9 57 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 15 PY 1999 VL 80 IS 6 BP 818 EP 822 DI 10.1002/(SICI)1097-0215(19990315)80:6<818::AID-IJC4>3.0.CO;2-T PG 5 WC Oncology SC Oncology GA 171BD UT WOS:000078841600004 PM 10074912 ER PT J AU Jia, L Wang, XW Harris, CC AF Jia, L Wang, XW Harris, CC TI Hepatitis B virus X protein inhibits nucleotide excision repair SO INTERNATIONAL JOURNAL OF CANCER LA English DT Article ID P53 TUMOR-SUPPRESSOR; HEPATOCELLULAR-CARCINOMA; DNA-DAMAGE; TFIIH; HBX; COMPLEX; ANTIGEN; CANCER; CELLS; GENE AB Human hepatitis B virus (HBV) is a major risk factor of human hepatocellular carcinoma. Both in vivo and in vitro studies have shown that HBV X protein (HBx) can bind to the p53 tumor-suppressor protein and interfere with the role that p53 plays in the cellular response to DNA damage. Our previous work has shown that HBx protein inhibits p53 sequence-specific transcriptional activation, p53-mediated apoptosis and p53 binding to the TFIIH transcription nucleotide excision repair (NER) factors, including XPB and XPD. To investigate whether HBx interferes with the NER pathway, we utilized cell-proliferation and colony-formation assays to determine if cells expressing HBx are more sensitive to UVC-induced DNA damage. NER was also measured by a plasmid host cell re-activation assay using a vector containing a luciferase reporter gene. UV-irradiated plasmids were transfected into a human RKO colon carcinoma cell line that contains wild-type (wt) p53 as well as its derivatives, either mutant p53-143(ala) (RKO-143(ala)) or human papillomavirus E6 (RKO-E6, a wt p53 protein that is rapidly degraded and non-functional). We found that cells expressing HBx are more sensitive to UVC induced killing. Moreover, expression of HBx resulted in a reduction of NER efficiency in RKO cells to 52 +/- 2% (compared with control), RKO-143(ala) cells to 46 +/- 3% and RKO-E6 cells to 60 +/- 3%. Similar results were also obtained with a HepG2 hepatoblastoma cell line carrying wt p53. In addition, we found that HBx bound directly to either XPB or XPD DNA helicase in vitro. Thus, our data indicate that HBx may interfere with the NER pathway through both p53-dependent and p53-independent mechanisms. Because HBx binds to TFIIH-associated proteins, we propose that HBx may interfere with the NER pathway also through binding to and altering the activities of helicases necessary for NER and, thereby, increase the mutation rate induced by chemical carcinogens, such as aflatoxin B-1, during human liver carcinogenesis. Int. J. Cancer 80:875-879, 1999, Published 1999 Wiley-Liss, Inc.dagger C1 NCI, Human Carcinogenesis Lab, Div Basic Sci, NIH, Bethesda, MD 20892 USA. RP Harris, CC (reprint author), NCI, Human Carcinogenesis Lab, Div Basic Sci, NIH, Bldg 37,Room 2C05,37 Convent Dr,MSC 4255, Bethesda, MD 20892 USA. RI Wang, Xin/B-6162-2009 NR 21 TC 121 Z9 133 U1 0 U2 5 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0020-7136 J9 INT J CANCER JI Int. J. Cancer PD MAR 15 PY 1999 VL 80 IS 6 BP 875 EP 879 DI 10.1002/(SICI)1097-0215(19990315)80:6<875::AID-IJC13>3.0.CO;2-Z PG 5 WC Oncology SC Oncology GA 171BD UT WOS:000078841600013 PM 10074921 ER PT J AU Becerra, S Hollyfield, JG Izal-Azcarate, I Perez-Mediavilla, LA AF Becerra, S Hollyfield, JG Izal-Azcarate, I Perez-Mediavilla, LA TI Pigment epithelium-derived factor (PEDF) has binding affinity for hyaluronan SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI, NIH, MSC 2740, Bethesda, MD 20205 USA. Cleveland Clin Fdn, Cleveland, OH 44195 USA. Univ Navarra, E-31080 Pamplona, Spain. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 42B2 BP S9 EP S9 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200043 ER PT J AU Bettelheim, FA Zigler, JS AF Bettelheim, FA Zigler, JS TI Kinetics and the mode of chaperoning of DTT denatured alpha-lactalbumin by alpha-crystallin. SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 Adelphi Univ, Garden City, NY 11530 USA. NEI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 11 BP S3 EP S3 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200012 ER PT J AU Brusie, SR Velez, G Wagner, DG Cupples, HP AF Brusie, SR Velez, G Wagner, DG Cupples, HP TI Epidemiology and long-term outcomes of open-globe missile injuries among children SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 Georgetown Univ, Ctr Sight, Washington, DC 20057 USA. NEI, NIH, Bethesda, MD 20205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 168B128 BP S32 EP S32 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200169 ER PT J AU Durbin, TD Robinson, MR Yuan, P Gogolak, L Sung, C Whitcup, SM AF Durbin, TD Robinson, MR Yuan, P Gogolak, L Sung, C Whitcup, SM TI Sustained-release devices with trimetrexate for the treatment of intraocular lymphoma: An in-vitro study SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NIH, Bioengn & Phys Sci Program, OD, Bethesda, MD 20892 USA. NEI, NIH, Bethesda, MD 20892 USA. NIH, Dept Pharm, Ctr Clin, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 451B411 BP S85 EP S85 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200451 ER PT J AU Gogolak, L Robinson, MR Yuan, P Aghera, A Sung, C Whitcup, SM AF Gogolak, L Robinson, MR Yuan, P Aghera, A Sung, C Whitcup, SM TI Sustained-release intraocular device for leflunomide; A new immunomodulating agent SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. NIH, Dept Pharm, Ctr Clin, Bethesda, MD 20892 USA. NIH, Phys Sci Program, OD, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 450B410 BP S85 EP S85 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200450 ER PT J AU Robinson, MR Yuan, P Gogolak, L Tansey, G Smith, J Whitcup, SM AF Robinson, MR Yuan, P Gogolak, L Tansey, G Smith, J Whitcup, SM TI Sustained-release biodegradable subconjunctival implants for long term delivery of cyclosporin A SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. NIH, Dept Pharm, Ctr Clin, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 449B409 BP S84 EP S84 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200449 ER PT J AU Velez, G Robinson, MR Durbin, T Yuan, P Sung, C Whitcup, SM AF Velez, G Robinson, MR Durbin, T Yuan, P Sung, C Whitcup, SM TI Thalidomide-sustained release devices for choroidal neovascularization; An in-vitro analysis SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. NIH, Bioengn & Phys Sci Program, OD, Bethesda, MD 20892 USA. NIH, Dept Pharmaceut, Ctr Clin, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD MAR 15 PY 1999 VL 40 IS 4 MA 448B408 BP S84 EP S84 PG 1 WC Ophthalmology SC Ophthalmology GA 178MF UT WOS:000079269200448 ER PT J AU Chang, JT Shevach, EM Segal, BM AF Chang, JT Shevach, EM Segal, BM TI Regulation of interleukin (IL)-12 receptor beta 2 subunit expression by endogenous IL-12: A critical step in the differentiation of pathogenic autoreactive T cells SO JOURNAL OF EXPERIMENTAL MEDICINE LA English DT Article DE autoimmunity; experimental allergic encephalomyelitis; T helper cell type 1 lymphocytes; interferon gamma; CD40 ligand ID EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS; MYELIN BASIC-PROTEIN; MULTIPLE-SCLEROSIS; IFN-GAMMA; LYMPHOCYTE-T; MICE; INDUCTION; ARTHRITIS; RESPONSES AB The interleukin (IL)-12 receptor (R)beta 2 subunit is the critical molecule involved in maintaining IL-12 responsiveness and controlling T helper cell type 1 lineage commitment. We demonstrate that IL-12 and interferon (IFN)-gamma play separate, but complementary, roles in regulating IL-12R beta 2 expression on antigen-specific CD4(+) T cells. These results are consistent with our previous observation that IL-12 can promote autoimmune disease through IFN-gamma-independent as well as -dependent pathways. Therefore, we compared the induction of IL-12 by, and the expression of the IL-12R beta 2 subunit on, myelin basic protein (MBP)-specific T cells from experimental allergic encephalomyelitis (EAE)-susceptible SJL (H-2(s)) mice and from EAE-resistant B10.S mice (H-2(s)). B10.S mice had an antigen-specific defect in their capacity to upregulate the IL-12R beta 2 subunit. Defective expression was not secondary to the production of suppressive cytokines, but to a failure of B10.S MBP-specific T cells to upregulate CD40 ligand expression and to induce the production of IL-12. IL-12R beta 2 expression as well as encephalitogenicity of these cells could be restored by the addition of IL-12. These results suggest that the development of immunotherapies that target the IL-12R beta 2 subunit may be useful for the treatment of autoimmune diseases. C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. RP Shevach, EM (reprint author), NIAID, Immunol Lab, NIH, Bldg 10,Rm 11N311,10 Ctr Dr,MSC 1892, Bethesda, MD 20892 USA. NR 46 TC 74 Z9 74 U1 0 U2 0 PU ROCKEFELLER UNIV PRESS PI NEW YORK PA 1114 FIRST AVE, 4TH FL, NEW YORK, NY 10021 USA SN 0022-1007 J9 J EXP MED JI J. Exp. Med. PD MAR 15 PY 1999 VL 189 IS 6 BP 969 EP 978 DI 10.1084/jem.189.6.969 PG 10 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 177RX UT WOS:000079225800009 PM 10075980 ER PT J AU Phung, QH Winter, DB Alrefai, R Gearhart, PJ AF Phung, QH Winter, DB Alrefai, R Gearhart, PJ TI Cutting edge: Hypermutation in Ig V genes from mice deficient in the MLH1 mismatch repair protein SO JOURNAL OF IMMUNOLOGY LA English DT Article ID MUTATION; CANCER; INVOLVEMENT; SEQUENCES; PMS2; CELL AB During somatic hypermutation of Ig V genes, mismatched nucleotide substitutions become candidates for removal by the DNA mismatch repair pathway. Previous studies have shown that V genes from mice deficient for the MSH2 and PMS2 mismatch repair proteins have frequencies of mutation that are comparable with those from wild-type (wt) mice; however, the pattern of mutation is altered. Because the absence of MSH2 and PMS2 produced different mutational spectra, we examined the role of another protein involved in mismatch repair, MLH1, on the frequency and pattern of hypermutation. MLH1-deficient mice were immunized with oxazolone Ag, and splenic B cells were analyzed for mutations in their V kappa Ox1 light chain genes. Although the frequency of mutation in MLH1-deficient mice was twofold lower than in wt mice, the pattern of mutation in Mlh1(-/-) clones was similar to wt clones. These findings suggest that the MLH1 protein has no direct effect on the mutational spectrum. C1 NIA, Mol Genet Lab, Gerontol Res Ctr, NIH, Baltimore, MD 21224 USA. Johns Hopkins Univ, Sch Med, Grad Program Immunol, Baltimore, MD 21205 USA. RP Gearhart, PJ (reprint author), NIA, Mol Genet Lab, Gerontol Res Ctr, NIH, 5600 Nathan Shock Dr, Baltimore, MD 21224 USA. NR 30 TC 50 Z9 50 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1999 VL 162 IS 6 BP 3121 EP 3124 PG 4 WC Immunology SC Immunology GA 175QB UT WOS:000079105000002 PM 10092760 ER PT J AU St Louis, DC Woodcock, JB Fransozo, G Blair, PJ Carlson, LM Murillo, M Wells, MR Williams, AJ Smoot, DS Kaushal, S Grimes, JL Harlan, DM Chute, JP June, CH Siebenlist, U Lee, KP AF St Louis, DC Woodcock, JB Fransozo, G Blair, PJ Carlson, LM Murillo, M Wells, MR Williams, AJ Smoot, DS Kaushal, S Grimes, JL Harlan, DM Chute, JP June, CH Siebenlist, U Lee, KP TI Evidence for distinct intracellular signaling pathways in CD34(+) progenitor to dendritic cell differentiation from a human cell line model SO JOURNAL OF IMMUNOLOGY LA English DT Article ID COLONY-STIMULATING FACTOR; PROTEIN-KINASE-C; TUMOR-NECROSIS-FACTOR; MOUSE BONE-MARROW; PERIPHERAL-BLOOD MONOCYTES; ACUTE MYELOGENOUS LEUKEMIA; RESISTANT SUBLINE KG-1A; PHORBOL ESTER RECEPTOR; NF-KAPPA-B; LANGERHANS CELLS AB Intracellular signals that mediate differentiation of pluripotent hemopoietic progenitors to dendritic cells (DC) are largely undefined. We have previously shown that protein kinase C (PKC) activation (with phorbol ester (PMA) alone) specifically induces differentiation of primary human CD34(+) hemopoietic progenitor cells (HPC) to mature DC. We now find that cytokine-driven (granulocyte-macrophage CSF and TNF-alpha) CD34(+) HPC --> DC differentiation is preferentially blocked by inhibitors of PKC activation. To further identify intracellular signals and downstream events important in CD34(+) HPC --> DC differentiation we have characterized a human leukemic cell line model of this process. The CD34(+) myelomonocytic cell line KG1 differentiates into dendritic-like cells in response to granulocyte-macrophage CSF plus TNF-alpha, or PMA (with or without the calcium ionophore ionomycin, or TNF-alpha), with different stimuli mediating different aspects of the process. Phenotypic DC characteristics of KG1 dendritic-like cells include morphology (loosely adherent cells with long neurite processes), MHC I+/MHC IIbright/CD83(+)/CD86(+)/CD14(-) surface Ag expression, and RelB and DC-CK1 gene expression. Functional DC characteristics include fluid phase macromolecule uptake (FITC-dextran) and activation of resting T cells. Comparison of KG1 to the PMA-unresponsive subline KG1a reveals differences in expression of TNF receptors 1 and 2; PKC isoforms alpha, beta I, beta II, and mu; and RelB, suggesting that these components/pathways are important for DC differentiation. Together, these findings demonstrate that cytokine or phorbol ester stimulation of KG1 is a model of human CD34(+) HPC to DC differentiation and suggest that specific intracellular signaling pathways mediate specific events in DC lineage commitment. C1 USN, Immune Cell Biol Program, Immune Suppress Branch, Med Res Inst, Bethesda, MD 20889 USA. Henry M Jackson Fdn Advancement Mil Med, US Mil HIV Res Program, Bethesda, MD 20889 USA. NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA. Uniformed Serv Univ Hlth Sci, Dept Internal Med, Bethesda, MD 20889 USA. RP Lee, KP (reprint author), USN, Immune Cell Biol Program, Immune Suppress Branch, Med Res Inst, Bldg 17,Room 214,8901 Wisconsin Ave, Bethesda, MD 20889 USA. EM leek@nmripo.nmri.nnmc.navy.mil NR 77 TC 73 Z9 74 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1999 VL 162 IS 6 BP 3237 EP 3248 PG 12 WC Immunology SC Immunology GA 175QB UT WOS:000079105000017 PM 10092775 ER PT J AU Zaks, TZ Chappell, DB Rosenberg, SA Restifo, NP AF Zaks, TZ Chappell, DB Rosenberg, SA Restifo, NP TI Fas-mediated suicide of tumor-reactive T cells following activation by specific tumor: Selective rescue by caspase inhibition SO JOURNAL OF IMMUNOLOGY LA English DT Article ID INFILTRATING LYMPHOCYTES; GRANZYME-B; IN-VIVO; APOPTOSIS; LIGAND; DEATH; MELANOMA; INTERLEUKIN-2; IMMUNOTHERAPY; CYTOTOXICITY AB CD8(+) T lymphocytes that specifically recognize tumor cells can be isolated and expanded ex vivo. While the lytic properties of these cells have been well described, their fate upon encounter with cognate tumor is not known. We performed reverse Cr-51 release assays in which the lymphocyte effecters rather than the tumor cell targets were radioactively labeled. We found that melanoma tumor cells caused the apoptotic death of tumor-specific T cells only upon specific MHC class I-restricted recognition. This death was entirely blockable by the addition of an Ab directed against the Fas death receptor (APO-1, CD95), Contrary to the prevailing view that tumor cells cause the death of anti-tumor T cells by expressing Fas ligand (FasL), our data suggested that Fast was instead expressed by T lymphocytes upon activation, While the tumor cells did not express Fast by any measure (including RT-PCR), functional Fast (as well as Fast mRNA) was consistently found on activated anti-tumor T cells. We could successfully block the activation-induced cell death with z-VAD-fmk, a tripeptide inhibitor of IL-1 beta-converting enzyme homologues, or with anti-Fas mAbs, Most importantly, these interventions did not inhibit T cell recognition as measured by IFN-gamma release, nor did they adversely affect the specific lysis of tumor cell targets. These results imply that Fas-mediated activation-induced cell death could be a limiting factor in the in vivo efficacy of adoptive transfer of class I-restricted CD8(+) T cells and provide a means of potentially enhancing their growth in vitro as well as their function in vivo. C1 NCI, Surg Branch, NIH, Bethesda, MD 20892 USA. NIH, Howard Hughes Med Inst, Res Scholars Program, Bethesda, MD 20814 USA. RP Zaks, TZ (reprint author), NCI, Surg Branch, NIH, Bldg 10,Rm 2B-46,9000 Rockville Pike, Bethesda, MD 20892 USA. RI Restifo, Nicholas/A-5713-2008; OI Restifo, Nicholas P./0000-0003-4229-4580 FU Intramural NIH HHS [Z99 CA999999, Z01 BC010763-01] NR 31 TC 108 Z9 109 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1999 VL 162 IS 6 BP 3273 EP 3279 PG 7 WC Immunology SC Immunology GA 175QB UT WOS:000079105000021 PM 10092779 ER PT J AU Perry, LL Su, H Feilzer, K Messer, R Hughes, S Whitmire, W Caldwell, HD AF Perry, LL Su, H Feilzer, K Messer, R Hughes, S Whitmire, W Caldwell, HD TI Differential sensitivity of distinct Chlamydia trachomatis isolates to IFN-gamma-mediated inhibition SO JOURNAL OF IMMUNOLOGY LA English DT Article ID GENITAL-TRACT INFECTION; TUMOR-NECROSIS-FACTOR; OUTER-MEMBRANE PROTEIN; NITRIC-OXIDE SYNTHASE; GENE KNOCKOUT MICE; MURINE INTERFERON-GAMMA; BLOOD-STAGE MALARIA; CD4(+) T-CELLS; INTRACELLULAR PATHOGENS; LISTERIA-MONOCYTOGENES AB Resistance to the mouse pneumonitis (MoPn) strain of Chlamydia trachomatis has been mapped to MHC class II-restricted, IL-12-dependent CD4(+) T cells that secrete a type 1 profile of proinflammatory cytokines, which includes IFN-gamma and TNF-alpha. The relative contribution of IFN-gamma is controversial, however, due to variation in results presented by different laboratories. To determine whether C, trachomatis strain differences contributed to this apparent conflict, the relative resistance of IFN-gamma-deficient mice to murine and human strains of C. trachomatis was compared. All human serovars were much more sensitive to the direct inhibitory actions of IFN-gamma than the MoPn strain. Furthermore, genital clearance of human serovar D in the C57BL/6 mouse was mediated by class II-independent mechanisms that probably involved local production of IFN-gamma by cells of the innate immune system. TNF-alpha also contributed indirectly to host resistance against all strains tested. The differential susceptibility of distinct C. trachomatis strains to effector cytokines such as IFN-gamma could not have been predicted by interstrain biologic variation or by the profile of cytokines stimulated during infection. These findings indicate that strain variation should be considered in situations where related isolates of a given parasite produce conflicting data in models of infection and immunity, They also suggest that stimulation of mucosal IFN-gamma activity is a relevant goal for a human chlamydial vaccine. C1 NIAID, Rocky Mt Lab, Intracellular Parasites Lab, Immunol Sect,NIH, Hamilton, MT 59840 USA. RP Caldwell, HD (reprint author), NIAID, Rocky Mt Lab, Intracellular Parasites Lab, Immunol Sect,NIH, 903 S 4th St, Hamilton, MT 59840 USA. NR 60 TC 83 Z9 85 U1 0 U2 2 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1999 VL 162 IS 6 BP 3541 EP 3548 PG 8 WC Immunology SC Immunology GA 175QB UT WOS:000079105000054 PM 10092812 ER PT J AU McDyer, JF Dybul, M Goletz, TJ Kinter, AL Thomas, EK Berzofsky, JA Fauci, AS Seder, RA AF McDyer, JF Dybul, M Goletz, TJ Kinter, AL Thomas, EK Berzofsky, JA Fauci, AS Seder, RA TI Differential effects of CD40 ligand/trimer stimulation on the ability of dendritic cells to replicate and transmit HIV infection: Evidence for CC-chemokine-dependent and -independent mechanisms SO JOURNAL OF IMMUNOLOGY LA English DT Article ID IMMUNODEFICIENCY-VIRUS TYPE-1; CD4(+) T-CELLS; PERIPHERAL-BLOOD; PRODUCTIVE INFECTION; ACTIVATION; ANTIGEN; LIGAND; INTERLEUKIN-12; CORECEPTORS; LYMPHOCYTES AB The role of exogenous stimulation of CD40 by CD40 ligand (CD40L) in dendritic cell (DC) maturation, CC-chemokine production, and CCR5 receptor expression was examined using a soluble trimeric CD40L agonist protein (CD40LT), Stimulation of monocyte-derived DCs with CD40LT enhanced the production of the CC-chemokines macrophage inflammatory protein (MIP)-1 alpha, MIP-1 beta, and RANTES and diminished surface expression of CCR5, Based on these findings, the functional role of CD40LT stimulation on the ability of DCs to replicate and transmit HIV viral infection was studied. The addition of CD40LT to cocultures of naive CD4(+) T cells and autologous DCs (T/DC) infected with the macrophage-tropic isolate, HIVBaL, caused a striking reduction in reverse transcriptase (RT) activity after 10 and 14 days of culture. The addition of a mixture of Abs against CC-chemokines abrogated the decrease in RT activity, demonstrating that the inhibitory effect mediated by CD40LT was CC-chemokine-dependent. In contrast, the presence of CD40LT in T/DC cocultures infected with the T cell-tropic isolate, HIVIIIB, caused an increase in RT activity that was CC-chemokine-independent. Of note, CD40LT stimulation also inhibited RT activity in cultures containing macrophage-tropic virus (HIVBaL)-infected DC only. However, in contrast to the results seen in the T/DC cocultures, CD40LT stimulation inhibited RT activity in cultures of DCs alone in a CC-chemokine-independent manner. Together, these results show that CD40LT stimulation of DCs suppresses HIV replication and transmission to CD4(+) T cells by two potentially different mechanisms. C1 NIAID, Clin Immunol Sect, Clin Invest Lab, NIH, Bethesda, MD 20892 USA. NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA. NCI, Mol Immunogenet & Vaccine Res Sect, Metab Branch, NIH, Bethesda, MD 20892 USA. Immunex Res & Dev Corp, Seattle, WA 98101 USA. RP Seder, RA (reprint author), NIAID, Clin Immunol Sect, Clin Invest Lab, NIH, 9000 Rockville Pike,Bldg 10,Room 11C215, Bethesda, MD 20892 USA. NR 42 TC 48 Z9 48 U1 0 U2 1 PU AMER ASSOC IMMUNOLOGISTS PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-1767 J9 J IMMUNOL JI J. Immunol. PD MAR 15 PY 1999 VL 162 IS 6 BP 3711 EP 3717 PG 7 WC Immunology SC Immunology GA 175QB UT WOS:000079105000076 PM 10092834 ER PT J AU Wang, ZZ Mathias, A Gautam, M Hall, ZW AF Wang, ZZ Mathias, A Gautam, M Hall, ZW TI Metabolic stabilization of muscle nicotinic acetylcholine receptor by rapsyn SO JOURNAL OF NEUROSCIENCE LA English DT Article DE nicotinic receptors; rapsyn; 43 kDa protein; receptor turnover; acetylcholine; neuromuscular junction; endplate; myotubes ID POSTSYNAPTIC 43-KDA PROTEIN; RAT SKELETAL-MUSCLE; 43 KDA PROTEIN; MOUSE MUSCLE; NEUROMUSCULAR-JUNCTIONS; TYROSINE PHOSPHORYLATION; NUCLEOTIDE-SEQUENCE; CYTOPLASMIC DOMAINS; EPSILON-SUBUNIT; LIGAND-BINDING AB Although the metabolic half-life of muscle endplate acetylcholine receptor (AChR) changes during development and after denervation in the adult, little is known about the molecular mechanisms that influence receptor stability. We have investigated the effect on AChR turnover of its interaction with rapsyn, a 43 kDa peripheral membrane protein that is closely associated with the AChR in muscle cells and is required for its clustering at endplates. Both in transfected COS cells and in cultured myotubes from rapsyn-negative and rapsyn-positive mice, we have found that the presence of rapsyn slows the turnover of AChRs by as much as twofold. The effect was similar for both embryonic (alpha(2)beta delta gamma) and adult (alpha(2)beta delta epsilon) AChRs and for AChRs whose beta subunit lacked a putative tyrosine phosphorylation site. Neither colchicine nor cytochalasin D altered AChR turnover or prevented the rapsyn effect. Mutant rapsyn proteins whose N-terminal myristoylation signal was eliminated, or whose C terminus or zinc-finger domains were deleted, failed to change the rate of receptor turnover. Each of these mutations affects the association of the AChR with rapsyn, suggesting that AChR stability is altered by interaction between the two proteins. Our results suggest that, in addition to its role in AChR clustering, rapsyn also functions to metabolically stabilize the AChR. C1 NIMH, Cell Biol Lab, NIH, Bethesda, MD 20892 USA. St Louis Univ, Sch Med, Dept Pharmacol & Physiol Sci, St Louis, MO 63104 USA. RP Wang, ZZ (reprint author), Univ Pittsburgh, Sch Med, Dept Neurobiol, 3500 Terrace St,E1440 BST, Pittsburgh, PA 15261 USA. NR 74 TC 34 Z9 34 U1 0 U2 0 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD MAR 15 PY 1999 VL 19 IS 6 BP 1998 EP 2007 PG 10 WC Neurosciences SC Neurosciences & Neurology GA 173BQ UT WOS:000078961400011 PM 10066253 ER PT J AU Fedirchuk, B Wenner, P Whelan, PJ Ho, S Tabak, J O'Donovan, MJ AF Fedirchuk, B Wenner, P Whelan, PJ Ho, S Tabak, J O'Donovan, MJ TI Spontaneous network activity transiently depresses synaptic transmission in the embryonic chick spinal cord SO JOURNAL OF NEUROSCIENCE LA English DT Article DE synaptic depression; spontaneous activity; spinal networks; synaptic currents; chick embryo; rhythmic activity ID LUMBOSACRAL MOTONEURONS; IN-VITRO; PLASTICITY; NEURONS; ORGANIZATION; CONNECTIONS; SYNAPSES; CORTEX; DRIVE AB We examined the effects of spontaneous or evoked episodes of rhythmic activity on synaptic transmission in several spinal pathways of embryonic day 9-12 chick embryos. We compared the amplitude of synaptic potentials evoked by stimulation of the ventrolateral funiculus (VLF), the dorsal or ventral roots, before and after episodes of activity. With the exception of the short-latency responses evoked by dorsal root stimulation. the potentials were briefly potentiated and then reduced for several minutes after an episode of rhythmic activity. Their amplitude progressively recovered in the interval between successive episodes. The lack of post-episode depression in the short-latency component of the dorsal root evoked responses is probably attributable to the absence of firing in cut muscle efferents during an episode of activity. The post-episode depression of VLF-evoked potentials was mimicked by prolonged stimulation of the VLF, subthreshold for an episode of activity. By contrast, antidromically induced motoneuron firing and the accompanying calcium entry did not depress VLF-evoked potentials recorded from the stimulated ventral root. In addition, post-episode depression of VLF-evoked synaptic currents was observed in voltage-clamped spinal neurons. Collectively, these findings suggest that somatic postsynaptic activity and calcium entry are not required for the depression. We propose instead that the mechanism may involve a form of long-lasting activity-induced synaptic depression, possibly a combination of transmitter depletion and ligand-induced changes in the postsynaptic current accompanying transmitter release. This activity-dependent depression appears to be an important mechanism underlying the occurrence of spontaneous activity in developing spinal networks. C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. Univ Manitoba, Dept Physiol, Winnipeg, MB R3E 3J7, Canada. Australian Natl Univ, Res Sch Biol Sci, Dept Dev Neurobiol, Canberra, ACT 2601, Australia. RP NINDS, Neural Control Lab, NIH, Bldg 49,Room 3A50,49 Convent Dr, Bethesda, MD 20892 USA. RI tabak, joel/K-1549-2013; o'donovan, michael/A-2357-2015; OI o'donovan, michael/0000-0003-2487-7547; Wenner, Peter/0000-0002-7072-2194 NR 43 TC 67 Z9 69 U1 1 U2 1 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD MAR 15 PY 1999 VL 19 IS 6 BP 2102 EP 2112 PG 11 WC Neurosciences SC Neurosciences & Neurology GA 173BQ UT WOS:000078961400021 PM 10066263 ER PT J AU Aley, KO Levine, JD AF Aley, KO Levine, JD TI Role of protein kinase A in the maintenance of inflammatory pain SO JOURNAL OF NEUROSCIENCE LA English DT Article DE adenylyl cyclase; cAMP; hyperalgesia; pain; protein kinase A; prostaglandin E-2 ID ROOT GANGLION NEURONS; APLYSIA SENSORY NEURONS; MECHANOSENSORY DISCHARGE; CATALYTIC SUBUNIT; HAIRY SKIN; KNEE-JOINT; RAT; SENSITIZATION; HYPERALGESIA; CELLS AB Although the initiation of inflammatory pain (hyperalgesia) has been demonstrated to require the cAMP second messenger signaling cascade, whether this mechanism and/or other mechanisms underlie the continued maintenance of the induced hyperalgesia is unknown. We report that injection of adenylyl cyclase inhibitors before but not after injection of direct-acting hyperalgesic agents (prostaglandin E-2 and purine and serotonin receptor agonists) resulted in reduction in hyperalgesia, evaluated by the Randall-Selitto paw-withdrawal test. In contrast, injection of protein kinase A (PKA) inhibitors either before or after these hyperalgesic agents resulted in reduced hyperalgesia, suggesting that hyperalgesia after its activation was maintained by persistent PKA activity but not by adenylyl cyclase activity. To evaluate further the role of PKA activity in the maintenance of hyperalgesia, we injected the catalytic subunit of PKA (PKACS) that resulted in hyperalgesia similar in magnitude to that induced by the direct-acting hyperalgesic agents but much longer in duration (>48 vs 2 hr). Injection of WIPTIDE (a PKA inhibitor) at 24 hr after PKACS reduced hyperalgesia, suggesting that PKACS hyperalgesia is not independently maintained by steps downstream from PKA. In summary, our results indicate that, once established, inflammatory mediator-induced hyperalgesia is no longer maintained by adenylyl cyclase activity but rather is dependent on ongoing PKA activity. An understanding of the mechanism maintaining hyperalgesia may provide important insight into targets for the treatment of persistent pain. C1 Univ Calif San Francisco, NIH, Pain Ctr, Dept Anat, San Francisco, CA 94143 USA. Univ Calif San Francisco, NIH, Pain Ctr, Dept Med, San Francisco, CA 94143 USA. Univ Calif San Francisco, NIH, Pain Ctr, Dept Oral Surg, San Francisco, CA 94143 USA. Univ Calif San Francisco, NIH, Pain Ctr, Grad Program Neurosci, San Francisco, CA 94143 USA. Univ Calif San Francisco, NIH, Pain Ctr, Biomed Sci Grad Program, San Francisco, CA 94143 USA. RP Levine, JD (reprint author), Univ Calif San Francisco, NIH, Pain Ctr, Dept Anat, C-522,Box 0440,521 Parnassus Ave, San Francisco, CA 94143 USA. FU NINDS NIH HHS [NS21647] NR 46 TC 144 Z9 153 U1 2 U2 4 PU SOC NEUROSCIENCE PI WASHINGTON PA 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA SN 0270-6474 J9 J NEUROSCI JI J. Neurosci. PD MAR 15 PY 1999 VL 19 IS 6 BP 2181 EP 2186 PG 6 WC Neurosciences SC Neurosciences & Neurology GA 173BQ UT WOS:000078961400029 PM 10066271 ER PT J AU Benech, JC Crispino, M Kaplan, BB Giuditta, A AF Benech, JC Crispino, M Kaplan, BB Giuditta, A TI Protein synthesis in presynaptic endings from squid brain: Modulation by calcium ions SO JOURNAL OF NEUROSCIENCE RESEARCH LA English DT Article DE nerve endings; presynaptic terminals; protein synthesis; Ca++; calmodulin; protein kinase C ID SARCOPLASMIC-RETICULUM; SYNAPTOSOMAL FRACTION; DEPENDENT REGULATION; ACTIVE POLYSOMES; NERVE-ENDINGS; PHOSPHORYLATION; NEURONS; INHIBITION; INITIATION; TRANSPORT AB Previous biochemical, autoradiographic, and ultrastructural data have shown that, in the synaptosomal fraction of the squid optic lobe, protein synthesis is largely due to the presynaptic terminals of the retinal photoreceptor neurons (Crispino ct al, [1993a] Mol, Cell, Neurosci, 4:366-374; Crispino et al, [1993b] J, Neurochem, 61:1144-1146; Crispino et al, [1997] J, Neurosci, 17:7694-7702), We now report that this process is close to its maximum at the basal concentration of cytosolic Ca++, and is markedly inhibited when the concentration of this ion is either decreased or increased, This conclusion is supported by the results of experiments with: 1) compounds known to increase the level of cytosolic Ca++, such as A23187, ionomycin, thapsigargin, and caffeine; 2) compounds sequestering cytosolic calcium ions such as BAPTA-AM; and 3) agents that block the role of Ca++ as second messenger, such as TFP and W7, which inhibit calmodulin, and calphostin, which inhibits protein kinase C, We conclude that variations in the level of cytosolic Ca++ induced in presynaptic terminals by neuronal activity may contribute to the modulation of the local synthesis of protein, (C) 1999 Wiley-Liss, Inc. C1 Univ Naples Federico II, Dipartimento Fisiol Gen & Ambientale, I-80134 Naples, Italy. Inst Invest Biol Clemente Estable, Montevideo, Uruguay. Fac Vet, Area Biofis, Montevideo, Uruguay. NIMH, Div Intramural Res Programs, NIH, Bethesda, MD 20892 USA. RP Giuditta, A (reprint author), Univ Naples Federico II, Dipartimento Fisiol Gen & Ambientale, Via Mezzocannone 8, I-80134 Naples, Italy. FU NINDS NIH HHS [NS30715] NR 41 TC 13 Z9 13 U1 0 U2 0 PU WILEY-LISS PI NEW YORK PA DIV JOHN WILEY & SONS INC, 605 THIRD AVE, NEW YORK, NY 10158-0012 USA SN 0360-4012 J9 J NEUROSCI RES JI J. Neurosci. Res. PD MAR 15 PY 1999 VL 55 IS 6 BP 776 EP 781 PG 6 WC Neurosciences SC Neurosciences & Neurology GA 182LL UT WOS:000079500400012 PM 10220118 ER PT J AU Exner, DV Dries, DL Waclawiw, MA Shelton, B Domanski, MJ AF Exner, DV Dries, DL Waclawiw, MA Shelton, B Domanski, MJ TI Beta-adrenergic blocking agent use and mortality in patients with asymptomatic and symptomatic left ventricular systolic dysfunction: A post hoc analysis of the studies of left ventricular dysfunction SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID CONGESTIVE-HEART-FAILURE; CONVERTING ENZYME-INHIBITORS; RANDOMIZED CLINICAL-TRIALS; PLASMA NOREPINEPHRINE; MYOCARDIAL-INFARCTION; SUDDEN-DEATH; MORBIDITY; BLOCKADE; THERAPY; CARDIOMYOPATHY AB OBJECTIVES This analysis was performed to assess whether beta-adrenergic blocking agent use is associated with reduced mortality in the Studies of Left Ventricular Dysfunction (SOLVD) and to determine if this relationship is altered by angiotensin-converting enzyme (ACE) inhibitor use. BACKGROUND The ability of beta-blockers to alter mortality in patients with asymptomatic left ventricular dysfunction is not well defined. Furthermore, the effect of beta-blocker use, in addition to an ACE inhibitor, on these patients has not been fully addressed. METHODS This retrospective analysis evaluated the association of baseline beta-blocker use with mortality in 4,223 mostly asymptomatic Prevention nial patients, and 2,567 symptomatic Treatment trial patients RESULTS The 1,015 (24%) Prevention trial patients and 197 (8%) Treatment trial patients receiving beta-blockers had fewer symptoms, higher ejection fractions and different use of medications than patients not receiving beta-blockers. On univariate analysis, beta-blocker use was associated with significantly lower mortality than nonuse in both trials. Moreover, a synergistic reduction in mortality with use of both a beta-blocker and enalapril was suggested in the Prevention trial. After adjusting for important prognostic variables with Cox multivariate analysis, the association of beta-adrenergic blocking agent use with reduced mortality remained significant for Prevention trial patients receiving enalapril. Lower rates of arrhythmic and pump failure death and risk of death or hospitalization for heart failure were observed. CONCLUSIONS The combination of a beta-blocker and enalapril was associated with a synergistic reduction in the risk of death in the SOLVD Prevention trial. (J Am Coil Cardiol 1999;33:916-23) (C) 1999 by the American College of Cardiology. C1 NHLBI, Clin Trials Sci Res Grp, Bethesda, MD 20892 USA. NHLBI, Off Biostat Res, Bethesda, MD 20892 USA. Wake Forest Univ, Bowman Gray Sch Med, Biostat Sect, Winston Salem, NC USA. RP Exner, DV (reprint author), NHLBI, Clin Trials Sci Res Grp, 2,6701 Rockledge Dr,Room 8146, Bethesda, MD 20892 USA. NR 41 TC 74 Z9 78 U1 1 U2 1 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 15 PY 1999 VL 33 IS 4 BP 916 EP 923 DI 10.1016/S0735-1097(98)00675-5 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 209TR UT WOS:000081065400003 PM 10091816 ER PT J AU Domanski, MJ Mitchell, GF Norman, JE Exner, DV Pitt, B Pfeffer, MA AF Domanski, MJ Mitchell, GF Norman, JE Exner, DV Pitt, B Pfeffer, MA TI Independent prognostic information provided by sphygmomanometrically determined pulse pressure and mean arterial pressure in patients with left ventricular dysfunction SO JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY LA English DT Article ID CONGESTIVE-HEART-FAILURE; MYOCARDIAL-INFARCTION; ESSENTIAL-HYPERTENSION; BLOOD-PRESSURE; CARDIOVASCULAR MORTALITY; NORMOTENSIVE SUBJECTS; CARDIAC-HYPERTROPHY; DISTENSIBILITY; HEMODYNAMICS; PULSATILE AB OBJECTIVES The purpose of this study was to evaluate the relationship of baseline pulse pressure and mean arterial pressure to mortality in patients with left Ventricular dysfunction. BACKGROUND Increased conduit vessel stiffness increases pulse pressure and pulsatile load, potentially contributing to adverse outcomes in patients with left ventricular dysfunction. METHODS Pulse and mean arterial pressure were analyzed for their effect on mortality, adjusting for other modifiers of risk, using Cox proportional hazards regression analysis of data collected from 6,781 patients randomized into the Studies of Left Ventricular Dysfunction trials. RESULTS Pulse and mean arterial pressure were related positively to each other, age, ejection fraction and prevalence of diabetes and hypertension and inversely to prior myocardial infarction and beta-adrenergic blocking agent use. Higher pulse pressure was associated with increased prevalence of female gender, greater calcium channel blocking agent, digoxin and diuretic use, lower heart rate and a higher rate of reported smoking history. Higher mean arterial pressure was associated with higher heart rate, lower calcium channel blocker and digoxin use and lower New York Heart Association functional class. Over a 61-month follow-up 1,582 deaths (1,397 cardiovascular) occurred. In a multivariate analysis adjusting for the above covariates and treatment assignment, higher pulse pressure remained an independent predictor of total and cardiovascular mortality (total mortality relative risk, 1.05 per 10 mm Hg increment, 95% confidence interval, 1.01 to 1.10; p = 0.02). Mean arterial pressure was inversely related to total and cardiovascular mortality (total mortality relative risk, 0.89; 95% confidence interval, 0.85 to 0.94; p < 0.0001). CONCLUSION One noninvasive blood pressure measurement provides two independent prognostic factors for survival. Increased conduit vessel stiffness, as assessed by pulse pressure, may contribute to increased mortality in patients with left ventricular dysfunction, independent of mean arterial pressure. (J Am Coil Cardiol 1999;33:951-8) (C) 1999 by the American College of Cardiology. C1 NHLBI, Clin Trials Grp, Bethesda, MD 20892 USA. Harvard Univ, Brigham & Womens Hosp, Sch Med, Div Cardiol, Boston, MA 02115 USA. NHLBI, Off Biostat Res, Bethesda, MD 20892 USA. Univ Michigan, Med Ctr, Div Cardiol, Ann Arbor, MI 48109 USA. RP Domanski, MJ (reprint author), NHLBI, Clin Trials Grp, 6701 Rockledge Dr,Rm 8146, Bethesda, MD 20892 USA. NR 44 TC 150 Z9 159 U1 0 U2 2 PU ELSEVIER SCIENCE INC PI NEW YORK PA 655 AVENUE OF THE AMERICAS, NEW YORK, NY 10010 USA SN 0735-1097 J9 J AM COLL CARDIOL JI J. Am. Coll. Cardiol. PD MAR 15 PY 1999 VL 33 IS 4 BP 951 EP 958 DI 10.1016/S0735-1097(98)00679-2 PG 8 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 209TR UT WOS:000081065400008 PM 10091821 ER PT J AU Levine, RL Berlett, BS Moskovitz, J Mosoni, L Stadtman, ER AF Levine, RL Berlett, BS Moskovitz, J Mosoni, L Stadtman, ER TI Methionine residues may protect proteins from critical oxidative damage SO MECHANISMS OF AGEING AND DEVELOPMENT LA English DT Article; Proceedings Paper CT 2nd International NILS Workshop on Longevity Sciences - Roles of Protein in Aging and Age-Associated Disorders CY NOV 29-30, 1997 CL OBU, JAPAN SP NILS DE methionine; antioxidants; protein oxidation; methionine sulfoxide; methionine sulfoxide reductase ID HUMAN ALPHA-1-PROTEINASE INHIBITOR; METAL-CATALYZED OXIDATION; GLUTAMINE-SYNTHETASE; ESCHERICHIA-COLI; SULFOXIDE FORMATION; BIOLOGICAL-ACTIVITY; DISSOCIATION; INACTIVATION; STRESS AB Cysteine and methionine are the two sulfur-containing residues normally found in proteins. Cysteine residues function in the catalytic cycle of many enzymes, and they form disulfide bonds which contribute to protein structure. In contrast: the key functions of methionine residues are not known. We propose that methionine residues constitute an important antioxidant defense mechanism. A variety of oxidants react readily with methionine to form methionine sulfoxide, and surface exposed methionine residues create an extremely high concentration of reactant, providing for efficient scavenging of oxidants. The effect of hydrogen peroxide exposure upon glutamine synthetase from Escherichia coli was studied as an in vitro model system. Eight of the sixteen methionine residues could be oxidized with little effect on activity. The oxidizable methionine residues were found to be relatively surface exposed while the intact residues were generally buried within the core of the protein. Further, the susceptible residues were physically arranged in an array which guarded the entrance to the active site. Methionine sulfoxide can be reduced back to methionine by the enzyme methionine sulfoxide reductase, providing a catalytic amplification of the antioxidant potential of each methionine residue. Given the importance of oxidative stress during aging, the potential function of methionine residues as antioxidants during aging should be investigated experimentally. Published by Elsevier Science Ireland Ltd. C1 NHLBI, Biochem Lab, NIH, Bethesda, MD 20892 USA. RP Levine, RL (reprint author), NHLBI, Biochem Lab, NIH, Bethesda, MD 20892 USA. EM rlevine@nih.gov RI Levine, Rodney/D-9885-2011 NR 28 TC 208 Z9 218 U1 3 U2 18 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0047-6374 J9 MECH AGEING DEV JI Mech. Ageing Dev. PD MAR 15 PY 1999 VL 107 IS 3 BP 323 EP 332 DI 10.1016/S0047-6374(98)00152-3 PG 10 WC Cell Biology; Geriatrics & Gerontology SC Cell Biology; Geriatrics & Gerontology GA 192VC UT WOS:000080099400009 PM 10360685 ER PT J AU Delongchamp, RR Malling, HV Chen, JB Heflich, RH AF Delongchamp, RR Malling, HV Chen, JB Heflich, RH TI An estimator of the mutant frequency in assays using transgenic animals SO MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS LA English DT Article DE Poisson distribution; maximum likelihood estimation; confidence interval; transgenic mutation assay ID MOUSE MUTATION ASSAY; STATISTICAL-ANALYSIS; SELECTION; MICE; LYMPHOCYTES; VARIABILITY; DESIGN AB The Poisson distribution is a fundamental probability model for count data, and is a natural model for the observed plaque counts in mutation assays using animals with lambda or Phi X174 transgenes. The Poisson likelihood for observed counts is a function of the mutant fraction, and it is straightforward to derive the associated maximum likelihood estimate of the mutant fraction and its variance. The estimate is easy to calculate, and if not the same, very similar to ad hoc estimates in current use. The model indicates the proper way to combine data from a number of plates, possibly prepared with different sample dilutions. The estimator of the mutant fraction is biased as a consequence of dividing by a random variable, the plaque count used to calculate the total recovered plaque-forming units. Fortunately, the bias becomes negligible as this count becomes large. On the other hand, increasing this count can increase the variance by decreasing the amount of sample assayed for mutant phages. Concurrent heed to the bias and the variance provides some guidance as to the optimum allocation of a sample into portions assayed for mutant phages and total recovered phages. The distribution of the estimate of the mutant fraction is related to the binomial distribution. This relationship implies a binomial distribution for the mutant count conditional on an overall count (either the sum of mutant and counted total plaques or the sum of counted mutant and non-mutant plaques). A special but important case occurs when each plate can be evaluated for mutant plaques and non-mutant plaques. Then, the observed proportion of mutants estimates the mutant fraction. More generally, the relationship to a binomial distribution provides a procedure for calculating a confidence interval. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Natl Ctr Toxicol Res, Div Biometry & Risk Assessment, Jefferson, AR 72079 USA. Natl Ctr Toxicol Res, Div Genet & Reprod Toxicol, Jefferson, AR 72079 USA. NIEHS, Mammalian Genet Grp, Toxicol Lab, Res Triangle Pk, NC 27709 USA. RP Delongchamp, RR (reprint author), Natl Ctr Toxicol Res, Div Biometry & Risk Assessment, HFT-20,3900 NCTR Rd, Jefferson, AR 72079 USA. NR 20 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1383-5718 J9 MUTAT RES-GEN TOX EN JI Mutat. Res. Genet. Toxicol. Environ. Mutagen. PD MAR 15 PY 1999 VL 440 IS 1 BP 101 EP 108 DI 10.1016/S1383-5718(99)00007-8 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology SC Biotechnology & Applied Microbiology; Genetics & Heredity; Toxicology GA 178ER UT WOS:000079253800010 PM 10095133 ER PT J AU Darden, T Perera, L Li, LP Pedersen, L AF Darden, T Perera, L Li, LP Pedersen, L TI New tricks for modelers from the crystallography toolkit: the particle mesh Ewald algorithm and its use in nucleic acid simulations SO STRUCTURE WITH FOLDING & DESIGN LA English DT Article ID MOLECULAR-DYNAMICS SIMULATION; TYPE-1 REVERSE-TRANSCRIPTASE; FAST MULTIPOLE METHOD; DOUBLE-STRANDED DNA; B-DNA; A-DNA; SYSTEMS; CRYSTAL; WATER; SUMS C1 NIEHS, Res Triangle Pk, NC 27709 USA. Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA. RP Darden, T (reprint author), NIEHS, POB 12233,MD-F008,RTP, Res Triangle Pk, NC 27709 USA. RI perera, Lalith/B-6879-2012; Pedersen, Lee/E-3405-2013 OI perera, Lalith/0000-0003-0823-1631; Pedersen, Lee/0000-0003-1262-9861 FU NHLBI NIH HHS [HL-06350] NR 53 TC 254 Z9 258 U1 2 U2 18 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0969-2126 J9 STRUCT FOLD DES JI Struct. Fold. Des. PD MAR 15 PY 1999 VL 7 IS 3 BP R55 EP R60 DI 10.1016/S0969-2126(99)80033-1 PG 6 WC Biochemistry & Molecular Biology; Biophysics; Cell Biology SC Biochemistry & Molecular Biology; Biophysics; Cell Biology GA 180XL UT WOS:000079411100003 PM 10368306 ER PT J AU Favaro, JP Maldarelli, F Arrigo, SJ Schmidt, MG AF Favaro, JP Maldarelli, F Arrigo, SJ Schmidt, MG TI Effect of Rev on the cytoplasmic localization of intron-containing human immunodeficiency virus type 1 RNA SO VIROLOGY LA English DT Article ID VIRAL MESSENGER-RNA; STRUCTURAL GENE-EXPRESSION; TRANS-ACTIVATOR GENE; NUCLEAR EXPORT; HTLV-III; PROTEIN EXPRESSION; TARGET SEQUENCE; HNRNP PROTEINS; HIV REV; IDENTIFICATION AB Human immunodeficiency virus type 1 (HIV-I) proteins are expressed from both intron-containing and completely spliced RNAs. Rev, an HIV-1 regulatory protein, is necessary for the expression of intron-containing RNAs. The effect of Rev on the subcellular localization of intron-containing HIV-I RNA was examined by in situ RNA hybridization. In the presence of Rev, intron-containing HIV-1 RNA accumulated at the nuclear membrane and within the cytoplasm of transfected cells. In the absence of Rev. intron-containing HIV-1 RNA accumulated within the nucleus. In similar to 20% of the cells transfected in the absence of Rev, intron-containing HIV-I RNA was also found in the cytoplasm. Differences in the subcytoplasmic localization of intron-containing HIV-1 RNA in the presence and absence of Rev were not observed using in situ RNA hybridization. To determine the effect of Rev on RNA localization within the cytoplasm, an extensive fractionation protocol involving both hypotonic and detergent lysis was used. In the presence of Rev, 40.9 +/- 4.6% of the cytoplasmic intron-containing HIV-I RNA was released by hypotonic lysis. A similar fractionation profile was seen for several other translated Viral and cellular RNAs. However, in the absence of Rev, only 16.5 +/- 5.1% of the cytoplasmic intron-containing HIV-1 RNA was released on hypotonic lysis (P < 0.005). Thus the cytoplasmic fractionation pattern of this RNA was altered in the absence of Rev. (C) 1999 Academic Press. C1 Med Univ S Carolina, Dept Microbiol & Immunol, Charleston, SC 29425 USA. NIAID, Mol Microbiol Lab, NIH, Bethesda, MD 20892 USA. FU NIAID NIH HHS [AI32415] NR 54 TC 10 Z9 11 U1 0 U2 0 PU ACADEMIC PRESS INC PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD MAR 15 PY 1999 VL 255 IS 2 BP 237 EP 249 DI 10.1006/viro.1998.9584 PG 13 WC Virology SC Virology GA 178JQ UT WOS:000079262900005 PM 10069949 ER PT J AU Oh, JD Vaughan, CL Chase, TN AF Oh, JD Vaughan, CL Chase, TN TI Effect of dopamine denervation and dopamine agonist administration on serine phosphorylation of striatal nmda receptor subunits SO BRAIN RESEARCH LA English DT Article DE Parkinson's disease; 6-hydroxydopamine lesion; basal ganglia; NMDA antagonist; KN93; motor response alterations ID D-ASPARTATE RECEPTOR; DEPENDENT PROTEIN-KINASE; MOTOR RESPONSE ALTERATIONS; LONG-TERM POTENTIATION; PARKINSONS-DISEASE; TYROSINE PHOSPHORYLATION; BASAL GANGLIA; GLUTAMATE ANTAGONIST; POSTSYNAPTIC DENSITY; RAT STRIATUM AB Sensitization of striatal N-methyl-D-aspartate (NMDA) receptors has been implicated in the pathogenesis of the response alterations associated with dopaminomimetic treatment of parkinsonian animals and patients. To determine whether serine phosphorylation of NMDA receptor subunits by activation of Ca2+/calmodulin-dependent protein-kinase Il (CaMKII) contributes to this process, we examined the effects of unilateral nigrostriatal ablation with B-hydroxydopamine and subsequent treatment with levodopa, SKF 38393 (D1-preferring dopamine agonist), or quinpirole (D2-preferring agonist) on motor responses and phosphorylation states. Three weeks of twice-daily levodopa administration to rats shortened the duration of their rotational response to levodopa or SKF 38393 challenge, but prolonged the duration of quinpirole-induced rotation. At the same time, levodopa treatment elevated serine phosphorylation of striatal NR2A (p < 0.02), but not that of NR2B subunits, without associated changes in subunit protein levels. Chronic treatment with SKF 38393 increased NR2A (p < 0.0001) but decreased NR2B (p < 0.004) serine phosphorylation. In contrast, chronic quinpirole treatment had no effect on NR2A but increased NR2B phosphorylation (p < 0.0001). The acute intrastriatal injection of the CaMKII inhibitor KN93 (1.0 mu g) not only normalized the levodopa-induced motor response alterations but also attenuated the D1 and D2 receptor-mediated serine phosphorylation of NR2A and NR2B subunits, respectively (p < 0.02). These results suggest that a CaMKII-mediated rise in serine phosphorylation of NMDA receptor subunits induced by intermittent stimulation of D1 or D2 dopaminergic receptors contributes to the apparent enhancement in striatal NMDA receptor sensitivity and thus to the dopaminergic response plasticity in levodopa-treated parkinsonian rats. (C) 1999 Elsevier Science B.V. All rights reserved. C1 NINCDS, Expt Therapeut Branch, NIH, Bethesda, MD 20892 USA. RP Chase, TN (reprint author), NINCDS, Expt Therapeut Branch, NIH, Bldg 10,Room 5C103,90900 Rockville Pike, Bethesda, MD 20892 USA. EM chase@helix.nih.gov NR 72 TC 104 Z9 107 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 13 PY 1999 VL 821 IS 2 BP 433 EP 442 DI 10.1016/S0006-8993(99)01121-X PG 10 WC Neurosciences SC Neurosciences & Neurology GA 175WL UT WOS:000079118700021 PM 10064831 ER PT J AU Fuchs, PN Roza, C Sora, I Uhl, G Raja, SN AF Fuchs, PN Roza, C Sora, I Uhl, G Raja, SN TI Characterization of mechanical withdrawal responses and effects of mu-, delta- and kappa-opioid agonists in normal and mu-opioid receptor knockout mice SO BRAIN RESEARCH LA English DT Article DE antinociception; morphine; opioid receptor; transgenic mouse ID MORPHINE-INDUCED ANALGESIA; RAT SPINAL-CORD; OPIATE RECEPTORS; NEUROPATHIC PAIN; DORSAL HORN; MOUSE; ANTINOCICEPTION; INVOLVEMENT; ENKEPHALIN; TOLERANCE AB Clinical and experimental observations suggest that opiates can exert different influences on the perception of stimuli from distinct sensory modalities. Thermally-induced nociception is classically responsive to opiate agonists. mu-Opioid receptor-deficient transgenic mice are more sensitive to thermal nociceptive stimuli and morphine fails to attenuate the nociceptive responses to thermal stimuli in these animals. To enhance our understanding of opiate influences on mechanical sensitivity, we have examined withdrawal responses to a sequence of ascending forces of mechanical stimuli in mice with normal (wild type), half-normal (heterozygous) and absent (homozygous) mu-opioid receptor levels. We report data from mice examined without drug pretreatment or following pretreatment with morphine, the selective kappa-opioid agonist, U50488H, and the selective delta-opioid agonist, DPDPE, Saline-pretreated mice of each genotype displayed similar, monotonically increasing frequency of withdrawal responses to the graded stimuli. Subcutaneously administered morphine produced a dose-dependent reduction in withdrawal responses in wild type and heterozygous mice, but had no significant effect in homozygous mice. Intraventricular administration of DPDPE also reduced the frequency of paw withdrawal (FPW) in wild type mice, but not in homozygous mice. In contrast, systemic U50488H produced a dose-dependent attenuation of paw withdrawal in both wild type and homozygous mice. These findings suggest that (1) interactions of endogenous peptides with mu-opioid receptors may not play a significant role in the response to mechanical stimuli in drug-free animals, and (2) deficiency of mu-opioid receptors has no functional consequence on the response to the prototypical kappa-opioid receptor agonist, but decreases responses to the prototypical mu- and delta-opioid receptor agonists. (C) 1999 Elsevier Science B.V. All rights reserved. C1 Johns Hopkins Univ, Sch Med, Dept Neurosurg, Baltimore, MD 21287 USA. Univ Alcala de Henares, Fac Med, Dept Fisiol, Madrid 28871, Spain. NIDA, Mol Neurobiol Branch, NIH, Baltimore, MD USA. Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21287 USA. Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21287 USA. Johns Hopkins Univ, Sch Med, Dept Anesthesiol & Crit Care Med, Baltimore, MD 21287 USA. RP Fuchs, PN (reprint author), Johns Hopkins Univ, Sch Med, Dept Neurosurg, 600 N Wolfe St,Meyer 5-109, Baltimore, MD 21287 USA. EM fuchs@uta.edu RI Roza, Carolina/H-3992-2015; OI Roza, Carolina/0000-0001-5757-9066; Yang, Shuman/0000-0002-9638-0890 FU NINDS NIH HHS [NS-26363] NR 38 TC 44 Z9 51 U1 1 U2 2 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 J9 BRAIN RES JI Brain Res. PD MAR 13 PY 1999 VL 821 IS 2 BP 480 EP 486 DI 10.1016/S0006-8993(99)01060-4 PG 7 WC Neurosciences SC Neurosciences & Neurology GA 175WL UT WOS:000079118700025 PM 10064835 ER PT J AU London, SJ Idle, JR Daly, AK Coetzee, GA AF London, SJ Idle, JR Daly, AK Coetzee, GA TI Genetic variation of CYP2A6, smoking, and risk of cancer SO LANCET LA English DT Article ID METABOLIC-ACTIVATION; POLYMORPHISM; SEQUENCE C1 NIEHS, Epidemiol Branch, Res Triangle Pk, NC 27709 USA. Inst Canc Res & Mol Biol, Trondheim, Norway. Univ Newcastle, Sch Med, Dept Pharmacol Sci, Newcastle Upon Tyne, Tyne & Wear, England. Univ So Calif, Sch Med, Norris Canc Ctr, Los Angeles, CA USA. RP London, SJ (reprint author), NIEHS, Epidemiol Branch, POB 12233,MD A3-05, Res Triangle Pk, NC 27709 USA. RI Daly, Ann/H-3144-2011; OI Daly, Ann/0000-0002-7321-0629; Idle, Jeff/0000-0002-6143-1520; London, Stephanie/0000-0003-4911-5290 NR 5 TC 105 Z9 109 U1 0 U2 1 PU LANCET LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0140-6736 J9 LANCET JI Lancet PD MAR 13 PY 1999 VL 353 IS 9156 BP 898 EP 899 DI 10.1016/S0140-6736(98)04984-8 PG 2 WC Medicine, General & Internal SC General & Internal Medicine GA 181BU UT WOS:000079421400018 PM 10093988 ER PT J AU Lloyd-Jones, DM Levy, D AF Lloyd-Jones, DM Levy, D TI Lifetime risk of developing coronary heart disease - Reply SO LANCET LA English DT Letter C1 NHLBI, Framingham Heart Study, Framingham, MA 01701 USA. RP Lloyd-Jones, DM (reprint author), NHLBI, Framingham Heart Study, Framingham, MA 01701 USA. RI Lloyd-Jones, Donald/C-5899-2009 NR 0 TC 0 Z9 0 U1 0 U2 1 PU LANCET LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0140-6736 J9 LANCET JI Lancet PD MAR 13 PY 1999 VL 353 IS 9156 BP 924 EP 924 DI 10.1016/S0140-6736(05)75028-5 PG 1 WC Medicine, General & Internal SC General & Internal Medicine GA 181BU UT WOS:000079421400050 ER PT J AU Anderson, BW Peoples, GE Castilleja, A Murray, JL Wharton, JT Bennink, JR Yewdell, JW Ioannides, CG AF Anderson, BW Peoples, GE Castilleja, A Murray, JL Wharton, JT Bennink, JR Yewdell, JW Ioannides, CG TI Rapid activation of CTL effector functions by HER-2 peptides reveals functionally distinct populations in healthy donors and breast cancer patients. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Bethesda, MD 20892 USA. Univ Texas, MD Anderson Canc Ctr, Houston, TX 77030 USA. RI yewdell, jyewdell@nih.gov/A-1702-2012 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A646 EP A646 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303729 ER PT J AU Arichi, T Saito, T Major, ME Shirai, M Feinstone, SM Berzofsky, JA AF Arichi, T Saito, T Major, ME Shirai, M Feinstone, SM Berzofsky, JA TI Dna vaccine for prophylaxis of hepatitis C virus (HCV) infection: Induction of HCV specific CTLs and protection from HCV-recombinant vaccinia infection in HLA-A2.1 transgenic mouse model. SO FASEB JOURNAL LA English DT Meeting Abstract C1 US FDA, Bethesda, MD 20892 USA. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A634 EP A634 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303656 ER PT J AU Armstrong, JM Chen, JF Apasov, S Smith, PT Chen, P Sitkovsky, M AF Armstrong, JM Chen, JF Apasov, S Smith, PT Chen, P Sitkovsky, M TI Gene dosage effect of adenosine signaling in murine thymocytes SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, LI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A622 EP A622 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303589 ER PT J AU Arudchandran, R Brown, MJ Song, JS Zhang, J Siraganian, RP Blank, U Rivera, J AF Arudchandran, R Brown, MJ Song, JS Zhang, J Siraganian, RP Blank, U Rivera, J TI Compartmentalization of a Vav functional complex to the plasma membrane is required for JNK activation in RBL-2H3 mast cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAMS, NIH, Bethesda, MD 20892 USA. NCI, NIH, Bethesda, MD 20892 USA. NIDCR, NIH, Bethesda, MD 20892 USA. Inst Pasteur, Paris, France. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A323 EP A323 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301869 ER PT J AU Bennett, TA Burt, HL Stetler-Stevenson, WG AF Bennett, TA Burt, HL Stetler-Stevenson, WG TI Disruption of cell adhesion to ECM proteins by a C-terminal MMP-2 fragment (PEX) and TIMP-2 SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A185 EP A185 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301073 ER PT J AU Bergmann-Leitner, ES Abrams, SI AF Bergmann-Leitner, ES Abrams, SI TI Fas-dependent and independent pathways in tumor cytolysis by human anti-ras oncogene-specific CTL. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Tumor Immunol & Biol Lab, NIH, Bethesda, MD 20892 USA. RI Bergmann-Leitner, Elke/B-3548-2011 OI Bergmann-Leitner, Elke/0000-0002-8571-8956 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A302 EP A302 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301747 ER PT J AU Birnbaum, LS Richardson, VM Alcasey, SK Blanton, J Walker, NJ Lucier, GW Lindros, KO Santostefano, MJ AF Birnbaum, LS Richardson, VM Alcasey, SK Blanton, J Walker, NJ Lucier, GW Lindros, KO Santostefano, MJ TI Localization of tcdd and effects on gene expression in isolated hepatocytes SO FASEB JOURNAL LA English DT Meeting Abstract C1 US EPA, NHEERL, Res Triangle Pk, NC 27711 USA. NIEHS, Res Triangle Pk, NC 27709 USA. Natl Publ Hlth Inst, Helsinki, Finland. Univ N Carolina, Curriculum Toxicol, Chapel Hill, NC 27599 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A154 EP A154 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300887 ER PT J AU Blood-Siegfried, J Patterson, R Dougherty, J Germolec, D AF Blood-Siegfried, J Patterson, R Dougherty, J Germolec, D TI A rodent model to study sudden infant death syndrome. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Duke Univ, Sch Nursing, Durham, NC USA. Natl Inst Environm Hlth Sci, Res Triangle Pk, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A631 EP A631 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303638 ER PT J AU Bochner, BS Zagorski, J Davenpeck, KL AF Bochner, BS Zagorski, J Davenpeck, KL TI Role for cytokines in lipopolysaccharide (LPS)-induced, selectin-mediated leukocyte rolling and adhesion in the rat mesenteric microcirculation. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Johns Hopkins Univ, Baltimore, MD 21224 USA. NIDR, Immunol Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A182 EP A182 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301051 ER PT J AU Boyce, BF Xing, L Franzoso, G Siebenlist, U AF Boyce, BF Xing, L Franzoso, G Siebenlist, U TI Regulation of osteoclast numbers by NF-kappa B SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Texas, Hlth Sci Ctr, San Antonio, TX 78284 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A580 EP A580 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303344 ER PT J AU Butera, RJ Johnson, SM Trouth, CO Rinzel, J Smith, JC AF Butera, RJ Johnson, SM Trouth, CO Rinzel, J Smith, JC TI Role of excitatory coupling and cellular heterogeneity in respiratory rhythm generation in the pre-Botzinger complex SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. NIDDK, Math Res Branch, NIH, Bethesda, MD 20892 USA. Howard Univ, Coll Med, Dept Physiol & Biophys, Washington, DC 20050 USA. NYU, Ctr Neural Sci, New York, NY 10013 USA. NYU, Courant Inst Math Sci, New York, NY 10013 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A492 EP A492 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302841 ER PT J AU Butera, RJ Wilson, CG Rinzel, J Smith, JC AF Butera, RJ Wilson, CG Rinzel, J Smith, JC TI Implementation of a fast dynamic clamp using real time linux SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. NIDDK, Res Branch, NIH, Bethesda, MD 20892 USA. NYU, Ctr Neural Sci, New York, NY 10013 USA. NYU, Courant Inst Math Sci, New York, NY 10013 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A424 EP A424 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302445 ER PT J AU Cao, WX Verma, M Germain, RN AF Cao, WX Verma, M Germain, RN TI Analysis of gene expression during thymic development SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Lymphocyte Biol Sect, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A617 EP A617 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303554 ER PT J AU Centola, M Wood, G Frucht, D Galon, J Aringer, M Farrell, C Kingma, D Horwitz, M Rosenberg, H Malech, H Kastner, D AF Centola, M Wood, G Frucht, D Galon, J Aringer, M Farrell, C Kingma, D Horwitz, M Rosenberg, H Malech, H Kastner, D TI The familial Mediterranean fever gene encodes a cytokine-responsive myeloid-specific nuclear inflammatory regulator SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A311 EP A311 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301799 ER PT J AU Chang, JT Segal, BM Shevach, EM AF Chang, JT Segal, BM Shevach, EM TI Role of IL-12 in regulation of IL-12R beta 2 subunit expression: Implications for autoimmunity SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A285 EP A285 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301647 ER PT J AU Charest, H Sedegah, M Yap, G Gazzinelli, RT Caspar, P Hoffman, SL Sher, A AF Charest, H Sedegah, M Yap, G Gazzinelli, RT Caspar, P Hoffman, SL Sher, A TI Th1 biased immune responses against malaria circum sporozoite protein induced by a stably transfected Toxoplasma gondii avirulent line. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. USN, Med Res Inst, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A633 EP A633 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303650 ER PT J AU Chen, LP Hardwick, JP Sitkovsky, MV Jacobson, KA AF Chen, LP Hardwick, JP Sitkovsky, MV Jacobson, KA TI Purification of recombinant mouse P2X(1) receptor SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, LBC, MRS, Bethesda, MD 20892 USA. NIAID, LI, NIH, Bethesda, MD 20892 USA. Northeastern Ohio Univ Coll Med & Pharm, Rootstown, OH 44272 USA. RI Jacobson, Kenneth/A-1530-2009 OI Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A465 EP A465 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302680 ER PT J AU Chung, DH Dorfman, JR Plaksin, D Belyakov, I Hunzinker, RD Berzofsky, JA Mage, MG Natarajan, K Margulies, DH AF Chung, DH Dorfman, JR Plaksin, D Belyakov, I Hunzinker, RD Berzofsky, JA Mage, MG Natarajan, K Margulies, DH TI Transgenic single chain beta 2-m-H-2D(d) molecules can educate CD8(+) T cells but not NK cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, YNCI, NIH, Bethesda, MD 20892 USA. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A308 EP A308 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301779 ER PT J AU Clay, T Custer, M Sachs, J Hwu, P Rosenberg, S Nishimura, M AF Clay, T Custer, M Sachs, J Hwu, P Rosenberg, S Nishimura, M TI Efficient transfer to human peripheral blood lymphocytes of a tumor antigen-reactive TCR confers anti-tumor reactivity. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Surg Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A304 EP A304 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301755 ER PT J AU Coligan, J Borrego, F Kabat, J Brooks, A AF Coligan, J Borrego, F Kabat, J Brooks, A TI Structural features controlling the interaction of the NK cell CD93/NKG2A receptor with HLA-E. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunogenet Lab, Rockville, MD 20852 USA. Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3052, Australia. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A307 EP A307 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301776 ER PT J AU Combs, CA Aletras, AH Balaban, RS AF Combs, CA Aletras, AH Balaban, RS TI Effect of muscle action and metabolic strain on oxidative metabolic capacity in human skeletal muscle. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, LCE, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A83 EP A83 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300479 ER PT J AU Danilkovitch, A Andreazzoli, D Lerman, M Leonard, EJ AF Danilkovitch, A Andreazzoli, D Lerman, M Leonard, EJ TI Transactivation of macrophage stimulating protein (MSP) receptor tyrosine kinase RON via integrins. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A469 EP A469 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302707 ER PT J AU Demarest, JF Jones, SB Ferrari, G Johnson, CJ Greenberg, M O'Brien, TO Blattner, W Edwards, J Bartholomew, C Cleghorn, F Weinhold, KJ AF Demarest, JF Jones, SB Ferrari, G Johnson, CJ Greenberg, M O'Brien, TO Blattner, W Edwards, J Bartholomew, C Cleghorn, F Weinhold, KJ TI Natural history of HIV-specific CTL activity during acute HIV infection: envelope CTL impact subsequent plasma viremia. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. Inst Human Virol, Baltimore, MD USA. Med Res Ctr, Port Spain, Trinid & Tobago. Duke Univ, Med Ctr, Durham, NC USA. RI Ferrari, Guido/A-6088-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A294 EP A294 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301695 ER PT J AU Dix, AR Morford, LA Zou, JP Shearer, GM Brooks, WH Roszman, TL AF Dix, AR Morford, LA Zou, JP Shearer, GM Brooks, WH Roszman, TL TI Monocyte mediated T-cell unresponsiveness SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Kentucky, Dept Microbiol & Immunol, Lexington, KY 40536 USA. NCI, Immunol Branch, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A610 EP A610 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303518 ER PT J AU Ecelbarger, CA Kim, GH Mitchell, CW Terris, J Wade, JB Masilamani, S Bradford, AD Knepper, MA AF Ecelbarger, CA Kim, GH Mitchell, CW Terris, J Wade, JB Masilamani, S Bradford, AD Knepper, MA TI Regulation of the abundance of sodium and urea transporters along the nephron by vasopressin. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, LKEM, NIH, Bethesda, MD 20892 USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A392 EP A392 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302261 ER PT J AU Fraundorfer, PE Beaven, MA AF Fraundorfer, PE Beaven, MA TI A cholera toxin (CTx)-sensitive phospholipase D (PLD) is regulated by subunits of heterotrimeric G-proteins in RBL-2H3 cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, LMI, NIH, Bethesda, MD 20892 USA. NR 3 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A137 EP A137 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300788 ER PT J AU Freeman, JG Ryan, JJ Hu-Li, J Stewart, JK AF Freeman, JG Ryan, JJ Hu-Li, J Stewart, JK TI Phenylethanolamine N-methyl transferase (PNMT) mRNA in lymphoid cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. NIAID, Immunol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A57 EP A57 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300326 ER PT J AU Freidag, BL Melton, G Collins, F Suen, W Seder, RA AF Freidag, BL Melton, G Collins, F Suen, W Seder, RA TI L-12 protein and immunostimulatory dna improve the efficacy of BCG vaccination in mice infected with mycobacterium tuberculosis. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A633 EP A633 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303655 ER PT J AU Gasior, M Ungard, JT Witkin, JM AF Gasior, M Ungard, JT Witkin, JM TI Evaluation of novel anticonvulsants as potential blockers of cocaine-induced convulsions SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDA, Drug Dev Grp, Neurosci Behav Branch, NIH, Lexington, KY 40583 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A475 EP A475 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302739 ER PT J AU Geiman, TM Durum, SK Muegge, K AF Geiman, TM Durum, SK Muegge, K TI LSH, a novel helicase family member, preferentially expressed in lymphoid tissue SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, SAIC Frederick, IRSP, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A615 EP A615 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303545 ER PT J AU Gerschenson, M Paik, CY Erhart, SW Poirier, MC AF Gerschenson, M Paik, CY Erhart, SW Poirier, MC TI Exposure of pregnant patas monkeys to 3 '-azido-2 ',3 '-dideoxythymidine (AZT) produces damaged mitochondria (MT) and abnormal energy metabolism in the fetal cardiac and skeletal muscle tissues SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A488 EP A488 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302815 ER PT J AU Gilbert, DL AF Gilbert, DL TI Paul Bert's relations with the Tissandier brothers SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Unit ROS, BNP, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A384 EP A384 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302218 ER PT J AU Gowda, DC Gluska, J von Halbeek, H Thotakura, RN Bredehorst, R Vogel, CW AF Gowda, DC Gluska, J von Halbeek, H Thotakura, RN Bredehorst, R Vogel, CW TI Structures of N-linked oligosaccharides from cobra venom factor. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Georgetown Univ, Dept Biochem & Mol Biol, Washington, DC 20007 USA. Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA. NIDDK, Mol & Cellular Endocrinol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A149 EP A149 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300861 ER PT J AU Guedez, L Kingma, DW Bennett, TA Yu, AE McMarlin, A Stetler-Stevenson, M Stetler-Stevenson, WG AF Guedez, L Kingma, DW Bennett, TA Yu, AE McMarlin, A Stetler-Stevenson, M Stetler-Stevenson, WG TI Tissue inhibitor of metalloproteinase(TIMP)-1 inhibits vascularization in vivo of subcutaneous lymphomas SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Pathol Lab, Bethesda, MD 20892 USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A362 EP A362 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302093 ER PT J AU Guo, DP Moro, S von Kugelgen, I Kim, YC Jacobson, KA AF Guo, DP Moro, S von Kugelgen, I Kim, YC Jacobson, KA TI Recognition of pyridoxal phosphate-related antagonists occurs with transmembrane domains of the human P2Y(1) receptor. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, Mol Recognit Sect, NIH, Bethesda, MD 20892 USA. RI Moro, Stefano/A-2979-2012; Jacobson, Kenneth/A-1530-2009 OI Moro, Stefano/0000-0002-7514-3802; Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 3 Z9 3 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A465 EP A465 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302683 ER PT J AU Gurunathan, S Stobie, L Prussin, C Wu, CY Glaicbenhaus, N Sacks, DL Seder, RA AF Gurunathan, S Stobie, L Prussin, C Wu, CY Glaicbenhaus, N Sacks, DL Seder, RA TI Vaccination with lack DNA induces protective immunity through generation of a unique population of CD8+T cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A632 EP A632 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303645 ER PT J AU Herreman, K Blackman, M Rubinstein, S Huang, X Pabst, K Harman, SM Caballero, B AF Herreman, K Blackman, M Rubinstein, S Huang, X Pabst, K Harman, SM Caballero, B TI Relationships of growth hormone secretion and serum IGF-I levels with protein turnover in healthy elderly subjects. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Johns Hopkins Med Inst, Ctr Human Nutr, Baltimore, MD 21205 USA. Johns Hopkins Med Inst, Dept Med, Baltimore, MD 21205 USA. NIA, Gerontol Res Ctr, Baltimore, MD 21205 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A82 EP A82 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300469 ER PT J AU Hilburger, ME Abrams, SI AF Hilburger, ME Abrams, SI TI Mechanisms of T cell-mediated immunity in a p53 tumor-antigen system. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Tumor Immunol & Biol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A645 EP A645 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303722 ER PT J AU Hirschberg, K Schwartz, JL AF Hirschberg, K Schwartz, JL TI Kinetic analysis of secretory traffic in living cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHD, CBMB, NIH, Bethesda, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A56 EP A56 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300320 ER PT J AU Hoegy, SE Stetler-Stevenson, WG AF Hoegy, SE Stetler-Stevenson, WG TI Characteristics of TIMP-2 cell surface binding proteins SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A520 EP A520 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302999 ER PT J AU Hoffmann, C Moro, S Jacobson, KA AF Hoffmann, C Moro, S Jacobson, KA TI Role of extracellular loops of G protein-coupled receptors in ligand recognition: A molecular modeling study of the human P2Y(1) receptor. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, Mol Recognit Sect, LBC, NIH, Bethesda, MD 20892 USA. RI Moro, Stefano/A-2979-2012; Jacobson, Kenneth/A-1530-2009 OI Moro, Stefano/0000-0002-7514-3802; Jacobson, Kenneth/0000-0001-8104-1493 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A464 EP A464 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302672 ER PT J AU Hoffmann, KF James, SL Cheever, AW Wynn, TA AF Hoffmann, KF James, SL Cheever, AW Wynn, TA TI Studies in double cytokine deficient mice reveal that optimal protective immunity against Schistosoma mansoni requires elements of both type-1 and type-2 cytokine responses SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, LPD, NIH, Bethesda, MD 20892 USA. RI Wynn, Thomas/C-2797-2011 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A637 EP A637 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303673 ER PT J AU Hong, HL Devereux, TR Boorman, GA Sills, RC AF Hong, HL Devereux, TR Boorman, GA Sills, RC TI Point mutations of K-ras and H-ras genes in mouse forestomach neoplasms induced by exposure to 1,3-butadiene, isoprene and chloroprene. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Natl Toxicol Program, NIEHS, RTP, Res Triangle Pk, NC 27709 USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A188 EP A188 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301085 ER PT J AU Hundley, TR Beaven, MA AF Hundley, TR Beaven, MA TI Regulation of COX-2 expression in a mast cell line. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, Lab Mol Immunol, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A469 EP A469 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302705 ER PT J AU Idris, AH Smith, HRC Ortaldo, JR Scalzo, AA Yokoyama, WM AF Idris, AH Smith, HRC Ortaldo, JR Scalzo, AA Yokoyama, WM TI Identification of the Chok gene product that regulates natural killing. SO FASEB JOURNAL LA English DT Meeting Abstract C1 CUNY Mt Sinai Sch Med, New York, NY 10029 USA. Washington Univ, HHMI, St Louis, MO 63110 USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. Univ Western Australia, Nedlands, WA 6907, Australia. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A307 EP A307 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301772 ER PT J AU Irvine, KR Parkhurst, M Shulman, EP Tupesis, J Custer, M Touloukian, C Gritz, L Sutmiller, R Offringa, R Rosenberg, SA Restifo, NP AF Irvine, KR Parkhurst, M Shulman, EP Tupesis, J Custer, M Touloukian, C Gritz, L Sutmiller, R Offringa, R Rosenberg, SA Restifo, NP TI Enhancing immune responses to "self" antigens by "anchor fixing" epitopes SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. Leiden Univ, Med Ctr, Leiden, Netherlands. Ther Biol, Cambridge, MA USA. RI Restifo, Nicholas/A-5713-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A645 EP A645 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303719 ER PT J AU Isogai, S Bennett, PN Weinstein, BM AF Isogai, S Bennett, PN Weinstein, BM TI The anatomy of the developing zebrafish vasculature revealed by three-dimensional confocal microangiography SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHD, Mol Genet Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A532 EP A532 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303069 ER PT J AU Iwasaki, A Kelsall, BL AF Iwasaki, A Kelsall, BL TI Distinct T cell priming by freshly isolated dendritic cells from Peyer's patch and spleen SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A606 EP A606 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303490 ER PT J AU Jacob, T Ascher, E Scheinman, M Hingorani, A Gade, P Fodera, M Tunio, A Seth, P AF Jacob, T Ascher, E Scheinman, M Hingorani, A Gade, P Fodera, M Tunio, A Seth, P TI P53 gene transfer to the injured rat carotid artery, its role in reducing neointimal formation and apoptosis SO FASEB JOURNAL LA English DT Meeting Abstract C1 Maimonides Med Ctr, Dept Vasc Surg, Brooklyn, NY 11219 USA. NCI, Med Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A129 EP A129 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300744 ER PT J AU Jourd'heuil, D Wink, DA Grisham, MB AF Jourd'heuil, D Wink, DA Grisham, MB TI Stability of nitrosothiols in human plasma. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Louisiana State Univ, Med Ctr, Shreveport, LA 71130 USA. NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A100 EP A100 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300576 ER PT J AU Kaneko, I Ichimiya, M Chang, SH Berezesky, IK Trump, BF Hussain, SP Harris, CC Amstad, PA AF Kaneko, I Ichimiya, M Chang, SH Berezesky, IK Trump, BF Hussain, SP Harris, CC Amstad, PA TI p53-Induced apoptosis is associated with the formation of reactive oxygen species (ROS) and increased expression of manganese superoxide dismutase (MnSOD). SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Maryland, Dept Pathol, Baltimore, MD 21201 USA. NCI, Human Carcinogenesis Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A518 EP A518 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302986 ER PT J AU Karnaky, KJ Masereeuw, R Piermarini, PM Renfro, JL Miller, DS AF Karnaky, KJ Masereeuw, R Piermarini, PM Renfro, JL Miller, DS TI Regulation of MRP2-mediated transport in shark rectal gland (RG) tubules. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Med Univ S Carolina, Dept Cell Biol & Anat, Charleston, SC 29425 USA. Univ Nijmegen, Dept Pharmacol, Nijmegen, Netherlands. Univ Florida, Dept Zool, Gainesville, FL 32611 USA. Univ Connecticut, Dept Physiol & Neurobiol, Storrs, CT 06269 USA. NIEHS, Lab Pharmacol & Chem, NIH, Res Triangle Pk, NC 27709 USA. Mt Desert Isl Biol Lab, Salsbury Cove, ME 04672 USA. RI Masereeuw, Roos/N-3582-2014 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A396 EP A396 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302285 ER PT J AU Kass, E Abrams, S Schlom, J Greiner, JW AF Kass, E Abrams, S Schlom, J Greiner, JW TI Breaking of tolerance and induction of protective host immunity in 45 CEA transgenic mice (CEA.Tg) immunized with a recombinant vaccinia-CEA virus (rV-CEA). SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Tumor Immunol & Biol Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A645 EP A645 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303723 ER PT J AU Kelly, AE Wang, HY Wang, LM Pierce, JH Jay, G Keegan, AD AF Kelly, AE Wang, HY Wang, LM Pierce, JH Jay, G Keegan, AD TI B cells from mice transgenic for IRS2 show reduced proliferation and IGE production SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, LCMB, Bethesda, MD 20892 USA. Amer Red Cross, Jerome H Holland Lab, Dept Immunol, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A319 EP A319 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301844 ER PT J AU Koh, CY Welniak, LA Murphy, WJ AF Koh, CY Welniak, LA Murphy, WJ TI Adoptive transfer of donor ALAK cells augments reconstitution and function of B cells post-allogeneic bone marrow transplantation in mice. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, FCRDC, Frederick, MD 21702 USA. RI Koh, Crystal/D-9986-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A643 EP A643 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303709 ER PT J AU Koh, CY George, T Bennett, M Blazar, BR Murphy, WJ AF Koh, CY George, T Bennett, M Blazar, BR Murphy, WJ TI Adoptive transfer of donor alak cells augments blockade of LY49 receptors using 5E6 mAb F(ab ')(2) fragments augments NK-mediated anti-tumor effects. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Bethesda, MD 21702 USA. Univ Texas, SW Med Ctr, Dallas, TX 75235 USA. Univ Minnesota, Ctr Canc, Minneapolis, MN 55455 USA. RI Koh, Crystal/D-9986-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A308 EP A308 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301778 ER PT J AU Korach, KS AF Korach, KS TI Estrogen receptor knock-out mice: Molecular and endocrine phenotypes SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS, Reprod & Dev Toxicol Lab, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A46 EP A46 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300263 ER PT J AU Koshiya, N Smith, JC AF Koshiya, N Smith, JC TI Inspiratory pacemaker neurons synchronize their bursting activities by glutamatergic excitatory synaptic connections in the pre-Botzinger complex (pre-BotC) in vitro. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Neural Control Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A493 EP A493 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302845 ER PT J AU Kotsonis, P Frey, A Frohlich, LG Hofmann, H Reif, A Wink, DA Feelisch, M Schmidt, HHHW AF Kotsonis, P Frey, A Frohlich, LG Hofmann, H Reif, A Wink, DA Feelisch, M Schmidt, HHHW TI Autoinhibition of neuronal NO synthase. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Wurzburg, Dept Pharmacol Toxicol, D-97078 Wurzburg, Germany. NCI, Bethesda, MD 20892 USA. Wolfson Inst Biomed Res, London W1P 9LN, England. RI Schmidt, Harald H. H. W./B-1549-2008 OI Schmidt, Harald H. H. W./0000-0003-0419-5549 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A132 EP A132 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300759 ER PT J AU Kruth, HS Chang, J Ifrim, I Zhang, WY AF Kruth, HS Chang, J Ifrim, I Zhang, WY TI Patocytosis: Macrophage uptake of hydrophobic materials into surface-connected compartments SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A201 EP A201 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301165 ER PT J AU Kuhns, DB Nelson, EL Gallin, JI AF Kuhns, DB Nelson, EL Gallin, JI TI IL-8 production in human neutrophils induced by fibrinogen and chemotactic doses of formyl peptide. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, NIH, Bethesda, MD 20814 USA. NCI, Frederick Canc Res & Dev Ctr, SAIC, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A316 EP A316 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301824 ER PT J AU Le Roith, D AF Le Roith, D TI Insulin-like growth factor I receptor signaling pathways SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, Diabet Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A77 EP A77 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300442 ER PT J AU LeBeau, AP Van Goor, F Stojilkovic, SS Sherman, A AF LeBeau, AP Van Goor, F Stojilkovic, SS Sherman, A TI Mathematical modelling of spontaneous action potentials in GT1 neurons SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, Math Res Branch, NIH, Bethesda, MD 20892 USA. NICHD, Endocrinol & Reprod Res Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A477 EP A477 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302753 ER PT J AU Lee, HW Hsu, CM Eiden, LE AF Lee, HW Hsu, CM Eiden, LE TI PACAP-induced up-regulation of VIP biosynthesis is sensitive to FK506 in bovine chromaffin cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A468 EP A468 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302699 ER PT J AU Lee, JK Sayers, TJ Brooks, AD Back, TC Wigginton, JM Wiltrout, RH AF Lee, JK Sayers, TJ Brooks, AD Back, TC Wigginton, JM Wiltrout, RH TI Endogenous IFN-gamma-dependent delay of in vivo tumor progression by Fas-overexpression on murine renal cancer. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick, MD 21702 USA. RI Sayers, Thomas/G-4859-2015 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A299 EP A299 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301725 ER PT J AU Lempicki, RA Kovacs, JA Baseler, MW Adelsberger, JW Metcalf, JA Dimitrov, DS Stevens, RA Lambert, LA Alvord, WG Lane, HC AF Lempicki, RA Kovacs, JA Baseler, MW Adelsberger, JW Metcalf, JA Dimitrov, DS Stevens, RA Lambert, LA Alvord, WG Lane, HC TI Impact of therapy on the kinetics of CD4(+) and CD8(+) T cell turnover in HIV-infected patients receiving HAART or IL-2 SO FASEB JOURNAL LA English DT Meeting Abstract C1 SAIC, CSP, Frederick, MD USA. DMS Inc, Newtown, CT 06470 USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21701 USA. NIAID, CMRS, NIH, Bethesda, MD 20892 USA. RI Lempicki, Richard/E-1844-2012 OI Lempicki, Richard/0000-0002-7059-409X NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A294 EP A294 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301700 ER PT J AU Liu, K Schoonmaker, MM Levine, BL June, CH Hodes, RJ Weng, NP AF Liu, K Schoonmaker, MM Levine, BL June, CH Hodes, RJ Weng, NP TI Constitutive and regulated expression of telomerase reverse transcriptase (hTERT) in human lymphocytes SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, GRC, LI, NIH, Baltimore, MD 21224 USA. HM Jackson Fdn AOMM, Bethesda, MD 20889 USA. NCI, EIB, Bethesda, MD 20892 USA. NIA, NIH, Bethesda, MD 20892 USA. RI Levine, Bruce/D-1688-2009 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A619 EP A619 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303566 ER PT J AU Lynch, RM Murphy, S Parnami, G Mejia, R AF Lynch, RM Murphy, S Parnami, G Mejia, R TI Influence of adenine nucleotide gradients on ATPase driven ion transport: Analytical and empirical analyses. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Arizona, Tucson, AZ 85724 USA. NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A75 EP A75 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300433 ER PT J AU Magone, MT Whitcup, SM Chan, CC Silver, PB Rizzo, LV AF Magone, MT Whitcup, SM Chan, CC Silver, PB Rizzo, LV TI IL-12 is essential for the induction of the late phase cellular infiltration in a murine model of allergic conjunctivitis. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NEI, NIH, Bethesda, MD 20892 USA. RI Rizzo, Luiz Vicente/B-4458-2009 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A338 EP A338 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301957 ER PT J AU Mahana, W Samaan, A Kindt, TJ AF Mahana, W Samaan, A Kindt, TJ TI Anti-keratin and anti-thyroglobulin autoimmune response in HTLV-1 infected rabbits. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, NIH, Rockville, MD 20852 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A631 EP A631 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303642 ER PT J AU Martin, KR Kari, FW Barrett, JC French, JE AF Martin, KR Kari, FW Barrett, JC French, JE TI Dietary N-acetyl-L-cysteine (NAC) protects against tumorigenesis in p53 haploinsufficient Tg.AC (v-Ha-ras) mice. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Natl Inst Environm Hlth Sci, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A586 EP A586 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303379 ER PT J AU Masereeuw, R Russel, FGM Miller, DS AF Masereeuw, R Russel, FGM Miller, DS TI Endothelin-1 controls ATP-driven drug transport in renal proximal tubule SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Nijmegen, Dept Pharmacol, Nijmegen, Netherlands. NIEHS, Chem Pharmacol Lab, NIH, Res Triangle Pk, NC 27709 USA. Mt Desert Isl Biol Lab, Salsbury Cove, ME 04672 USA. RI Russel, Frans/B-3184-2014; Masereeuw, Roos/N-3582-2014 OI Russel, Frans/0000-0002-7959-2314; NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A61 EP A61 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300351 ER PT J AU Matsui, S Ahlers, JD Vortmeyer, AO Carbone, DP Liotta, LA Berzofsky, JA AF Matsui, S Ahlers, JD Vortmeyer, AO Carbone, DP Liotta, LA Berzofsky, JA TI The role of CD8+CTL lytic activity and interferon-gamma production in tumor regression and CD4 T cells in facilitating recurrence of tumor resistant to CTL SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Metab Branch, NIH, Bethesda, MD 20892 USA. NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. Vanderbilt Univ, Sch Med, Vanderbilt Canc Ctr, Dept Med, Nashville, TN 37212 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A305 EP A305 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301763 ER PT J AU McDyer, JF Dybul, M Goletz, TJ Kinter, AL Thomas, EK Berzofsky, JA Fauci, AS Seder, RA AF McDyer, JF Dybul, M Goletz, TJ Kinter, AL Thomas, EK Berzofsky, JA Fauci, AS Seder, RA TI Differential effects of CD40 ligand/trimer stimulation on the ability of dendritic cells to replicate and transmit HIV infection: Evidence for CC-chemokine-dependent and -independent mechanisms. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Clin Invest Lab, NIH, Bethesda, MD 20892 USA. NIAID, Immunoregulat Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A316 EP A316 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301828 ER PT J AU Mejia, R Knepper, MA Wade, JB AF Mejia, R Knepper, MA Wade, JB TI Immunomorphometric study of rat renal inner medulla SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. Univ Maryland, College Pk, MD 20742 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A393 EP A393 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302271 ER PT J AU Michea, L Ferguson, D Peters, E Kirby, M Burg, M AF Michea, L Ferguson, D Peters, E Kirby, M Burg, M TI High NaCl and urea induce transient dose-dependent G(2)-S cell cycle delay and apoptosis in renal inner medullary collecting duct cells (mIMCD). SO FASEB JOURNAL LA English DT Meeting Abstract C1 NHLBI, Kidney & Electrolyte Metab Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A387 EP A387 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302234 ER PT J AU Nakamura, MC Linnemeyer, PA Niemi, EC Mason, LH Ortaldo, JR Ryan, JC Seaman, WE AF Nakamura, MC Linnemeyer, PA Niemi, EC Mason, LH Ortaldo, JR Ryan, JC Seaman, WE TI Mouse LY-49D recognizes H-2D(d) and activates NK cell cytotoxicity. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Calif San Francisco, San Francisco, CA 94121 USA. SFVAMC, San Francisco, CA 94121 USA. NCI, Frederick Canc Res & Dev Ctr, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A305 EP A305 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301765 ER PT J AU Natarajan, K Boyd, LF Schuck, P Matsumoto, N Yokoyama, WM Eilat, D Margulies, DH AF Natarajan, K Boyd, LF Schuck, P Matsumoto, N Yokoyama, WM Eilat, D Margulies, DH TI Recombinant inhibitory NK cell receptor Ly-49A binds bacterially expressed and in vitro refolded H-2Dd/peptide complexes. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, LI, NIH, Bethesda, MD 20892 USA. NIH, BSPS, OD, Bethesda, MD 20892 USA. Washington Univ, Sch Med, St Louis, MO 63110 USA. RI Margulies, David/H-7089-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A308 EP A308 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301783 ER PT J AU Nicosia, RF Zhu, WH Guo, X Stetler-Stevenson, W AF Nicosia, RF Zhu, WH Guo, X Stetler-Stevenson, W TI Regulation of vascular growth and regression by matrix metalloproteinases in the rat aorta model of angiogenesis. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Med Coll Penn & Hahnemann Univ, Philadelphia, PA 19102 USA. NCI, Bethesda, MD 20892 USA. RI Stetler-Stevenson, William/H-6956-2012 OI Stetler-Stevenson, William/0000-0002-5500-5808 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A527 EP A527 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303041 ER PT J AU Oriji, GK AF Oriji, GK TI Nitric oxide in cyclosporine A-induced hypertension: Endothelin receptor gene expression. SO FASEB JOURNAL LA English DT Meeting Abstract C1 William Paterson Univ, Coll Sci & Hlth, Dept Biol, Wayne, NJ 07470 USA. NHLBI, Hypertens Endocrine Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A112 EP A112 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300643 ER PT J AU Overwijk, WW Lee, DS Surman, DR Irvine, KR Chan, C Carroll, MW Moss, B Rosenberg, SA Restifo, NP AF Overwijk, WW Lee, DS Surman, DR Irvine, KR Chan, C Carroll, MW Moss, B Rosenberg, SA Restifo, NP TI Induction of autoimmune vitiligo and tumor destruction after vaccination with a "self" antigen is dependent on CD4+T lymphocytes SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. NEI, Immunol Lab, Bethesda, MD 20892 USA. NIAID, Viral Dis Lab, NIH, Bethesda, MD 20892 USA. RI Restifo, Nicholas/A-5713-2008 NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A299 EP A299 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301728 ER PT J AU Panelli, MC Riker, AI Kammula, U Wang, E Lee, KH Rosenberg, SA Marincola, FM AF Panelli, MC Riker, AI Kammula, U Wang, E Lee, KH Rosenberg, SA Marincola, FM TI Expansion of tumor infiltrating lymphocytes (TIL) /tumor pairs from fine needle aspirates (FNA) of melanoma metastases. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Surg Branch, Bethesda, MD 20892 USA. DTM, CC, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A304 EP A304 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301759 ER PT J AU Park, JB Levine, M AF Park, JB Levine, M TI Characterization of the promoter of human ribonucleotide reductase SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIDDK, BHNRC, ARS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A357 EP A357 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302063 ER PT J AU Pinto, LA Berzofsky, JA Fowke, KR Little, RF Merced-Galindez, F Humphrey, R Ahlers, J Dunlop, N Nara, P Shearer, GM Yarchoan, R AF Pinto, LA Berzofsky, JA Fowke, KR Little, RF Merced-Galindez, F Humphrey, R Ahlers, J Dunlop, N Nara, P Shearer, GM Yarchoan, R TI Anti-HIV immunity following HIV-envelope peptide immunization SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A296 EP A296 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301708 ER PT J AU Preusch, PC AF Preusch, PC TI Alternative career paths - What does an NIH administrator do for a living? SO FASEB JOURNAL LA English DT Meeting Abstract C1 Natl Inst Gen Med Sci, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A238 EP A238 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301371 ER PT J AU Raziuddin, A Bennett, M Winkler-Pickett, R Ortaldo, J Murphy, WJ AF Raziuddin, A Bennett, M Winkler-Pickett, R Ortaldo, J Murphy, WJ TI Synergistic effects of in vivo depletion of Ly-49A(+) and Ly-49G2(+) NK cell subsets in the rejection of H-2(b) bone marrow cell allografts. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, DBS, Frederick, MD 21702 USA. Univ Texas, SW Med Ctr, Dallas, TX 75235 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A307 EP A307 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301773 ER PT J AU Rivoltini, L Squarcina, P Loftus, DJ Castelli, C Tarsini, P Mazzocchi, A Rini, F Viggiano, V Belli, F Parmiani, G AF Rivoltini, L Squarcina, P Loftus, DJ Castelli, C Tarsini, P Mazzocchi, A Rini, F Viggiano, V Belli, F Parmiani, G TI A superagonist variant of peptide MART-1/MELAN A(27-35) elicits anti-melanoma CD8+T cells with enhanced functional characteristics. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Ist Nazl Tumori, I-20133 Milan, Italy. NCI, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A304 EP A304 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301756 ER PT J AU Roman, BL Pham, VN Bennett, PE Weinstein, BM AF Roman, BL Pham, VN Bennett, PE Weinstein, BM TI Positional cloning of a gene responsible for a localized defect in the patterning of the zebrafish dorsal aorta SO FASEB JOURNAL LA English DT Meeting Abstract C1 NICHD, LMG, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A532 EP A532 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303065 ER PT J AU Ryan, RR Mantey, SA Pradhan, TK Battey, JF Jensen, RT AF Ryan, RR Mantey, SA Pradhan, TK Battey, JF Jensen, RT TI Comparative pharmacology of PD 108368, a non-peptide neuromedin B antagonist. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 2 Z9 2 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A466 EP A466 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302685 ER PT J AU Samaan, A Thibedeau, J Checchi, F Kindt, TJ AF Samaan, A Thibedeau, J Checchi, F Kindt, TJ TI Trafficking of a mixed isotypic pair between HLA-DR alpha and a chimeric DO beta chain. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Immunogenet Lab, NIH, Rockville, MD 20852 USA. Univ Montreal, Lab Mol Immunol, Montreal, PQ H3C 3J7, Canada. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A278 EP A278 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301607 ER PT J AU Schaffer, WT AF Schaffer, WT TI Biomedical graduate and postdoctoral training programs in the twenty-first century: Changing the incentives SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A378 EP A378 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302181 ER PT J AU Schito, M Hieny, S Sousa, CRE Sher, A AF Schito, M Hieny, S Sousa, CRE Sher, A TI In vivo dendritic cell migration in response to injected Toxoplasma gondii extract is dependent on TNF-receptor expression. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, LPD, NIH, Bethesda, MD 20892 USA. Imperial Canc Res Fund, London WC2A 3PX, England. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A280 EP A280 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301619 ER PT J AU Schreurs, BG AF Schreurs, BG TI Is there a role for long-term depression in classical conditioning? SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, NIH, Lab Adapt Syst, Behav Neurosci Unit, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A140 EP A140 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300807 ER PT J AU Schwartzberg, PL Schaeffer, EM Debnath, J McVicar, D Littman, D Varmus, HE Lenardo, MJ AF Schwartzberg, PL Schaeffer, EM Debnath, J McVicar, D Littman, D Varmus, HE Lenardo, MJ TI Altered T cell development in mice lacking the Tec kinases, Rlk/Txk and Itk SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Bethesda, MD 20892 USA. NIAID, NIH, Bethesda, MD 20892 USA. NYU, New York, NY 10012 USA. NIH, HHMI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A624 EP A624 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303597 ER PT J AU Shen, W Su, S Gong, W Dunlop, NM Wang, JM AF Shen, W Su, S Gong, W Dunlop, NM Wang, JM TI Down-regulation of CCR5 in human monocytes by bacterial chemotactic formyl peptide is associated with receptor phosphorylation SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, IRSP, SAIC Frederick, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, Mol Immunoregulat Lab, Frederick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A316 EP A316 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301825 ER PT J AU Sher, L Enoch, MA Mazzanti, CM Hardin, TA Greenberg, BD Murphy, DL Goldman, D Rosenthal, NE AF Sher, L Enoch, MA Mazzanti, CM Hardin, TA Greenberg, BD Murphy, DL Goldman, D Rosenthal, NE TI The role of the 5-HTT length promoter repeat polymorphism and the 5-HT2A,-1438G/A, promoter polymorphism in the etiology of seasonality and seasonal affective disorder SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIMH, Sect Biol Rhythms, Bethesda, MD 20892 USA. NIAAA, Neurogenet Lab, Rockville, MD 20852 USA. NIMH, Clin Sci Lab, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A85 EP A85 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300489 ER PT J AU Singer, SM Nash, TE AF Singer, SM Nash, TE TI Immunity to Giardia lamblia: Roles for mast cells and T cells SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIAID, Parasit Dis Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A641 EP A641 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303697 ER PT J AU Su, SB Gong, WH Gao, JL Shen, WP Grimm, MC Murphy, PM Oppenheim, JJ Wang, JM AF Su, SB Gong, WH Gao, JL Shen, WP Grimm, MC Murphy, PM Oppenheim, JJ Wang, JM TI T20/DP 178, an ectodomain peptide of HIV-1 gp41, is a potent activator of human phagocyte N-formyl peptide receptor SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Frederick Canc Res & Dev Ctr, Mol Immunoregulat Lab, Div Basic Sci, Frederick, MD 21702 USA. NCI, Frederick Canc Res & Dev Ctr, Intramural Res Support Program, SAIC, Frederick, MD 21702 USA. NIAID, Host Def Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A293 EP A293 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301689 ER PT J AU Swann, PG Odom, S Szallasi, Z Blumberg, PM Draber, P Rivera, J AF Swann, PG Odom, S Szallasi, Z Blumberg, PM Draber, P Rivera, J TI Phosphorylation of threonine 60 in the Fc epsilon RI gamma ITAM is required for complete activation of Syk and mast cell responses. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Acad Sci Czech Republ, Inst Mol Genet, Prague, Czech Republic. NCI, NIH, Bethesda, MD 20892 USA. NIAMS, NIH, Bethesda, MD USA. USUHS, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A323 EP A323 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033301866 ER PT J AU Sweet, DH Walden, R Pritchard, JB AF Sweet, DH Walden, R Pritchard, JB TI Functional evaluation of rOCT2 stably expressed in madin darby canine kidney (MDCK) cells. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIEHS, Lab Pharmacol & Chem, NIH, Res Triangle Pk, NC 27709 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A61 EP A61 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033300353 ER PT J AU Thomas, A Fields, R Jeong, S Gainer, H AF Thomas, A Fields, R Jeong, S Gainer, H TI Use of the gene gun as a complement to transgenic mice to study neuronal gene expression. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, Neurochem Lab, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A477 EP A477 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302754 ER PT J AU Torday, JS Londos, C Schultz, C Rubin, LP AF Torday, JS Londos, C Schultz, C Rubin, LP TI Distension of the developing lung triggers coordinate, growth factor-mediated cell-cell signal transduction. SO FASEB JOURNAL LA English DT Meeting Abstract C1 Univ Calif Los Angeles, Harbor Med Ctr, Dept Pediat, Torrance, CA 90502 USA. NIDDK, NIH, Bethesda, MD 20814 USA. Brown Univ, Dept Pediat, Providence, RI 02912 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A354 EP A354 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033302050 ER PT J AU Tschetter, JR Shearer, GM AF Tschetter, JR Shearer, GM TI The role of MLS in generating acute and chronic parent-into-F1 graft versus host disease (GVHD) SO FASEB JOURNAL LA English DT Meeting Abstract C1 NCI, Expt Immunol Branch, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A614 EP A614 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303541 ER PT J AU Turner, RV Biddison, WE AF Turner, RV Biddison, WE TI Molecular definition of class I MHC restriction of a peptide-specific alpha beta T cell receptor. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NINDS, NIH, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A625 EP A625 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303602 ER PT J AU Ullmann, CD Zea, A Taub, D Ochoa, A Longo, DL AF Ullmann, CD Zea, A Taub, D Ochoa, A Longo, DL TI Decreased expression of the IKK alpha and beta correlate with the lack of NF kappa B p65 nuclear translocation in CD4(+) T cells of tumor bearing hosts. SO FASEB JOURNAL LA English DT Meeting Abstract C1 NIA, Immunol Lab, Baltimore, MD 21224 USA. Louisiana State Univ, New Orleans, LA 70112 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD MAR 12 PY 1999 VL 13 IS 4 SU S BP A613 EP A613 PN 1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 226QW UT WOS:000082033303531 ER EF