FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Hirneisen, KA Sharma, M Kniel, KE AF Hirneisen, Kirsten A. Sharma, Manan Kniel, Kalmia E. TI Human Enteric Pathogen Internalization by Root Uptake into Food Crops SO FOODBORNE PATHOGENS AND DISEASE LA English DT Review ID ESCHERICHIA-COLI O157-H7; CONTAMINATED IRRIGATION WATER; LACTUCA-SATIVA L.; SALMONELLA-ENTERICA; GREEN ONIONS; SPINACH PLANTS; TOMATO PLANTS; WASTE-WATER; LETTUCE; O157H7 AB With an increasing number of outbreaks and illnesses associated with produce contaminated before harvest, understanding the potential and mechanisms of produce contamination by enteric pathogens can aid in the development of preventative and post-harvest processing measures to reduce microbial populations. Enteric pathogens localized at subsurface sites on leafy green plant tissue prevent their removal during washing and inactivation by sanitizers. Root uptake of enteric pathogens and subsequent internalization has been a large area of research with results varying due to differences in experimental design, systems tested, and pathogens and crops used. The potential for uptake of foodborne pathogen, both bacterial and viral, through roots into food crops is reviewed. Various factors shown to affect the ability of human pathogens to internalize include growth substrate (soil vs. hydroponic solution), plant developmental stage, pathogen genus and/or strain, inoculum level, and plant species and cultivar. Several mechanisms of internalization (``active'' vs. ``passive'') of bacteria to plant roots have also been hypothesized. C1 [Hirneisen, Kirsten A.; Kniel, Kalmia E.] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. [Sharma, Manan] ARS, Environm Microbial & Food Safety Lab, USDA, Anim & Nat Resources Inst, Beltsville, MD USA. RP Kniel, KE (reprint author), Univ Delaware, Dept Anim & Food Sci, 044 Townsend Hall, Newark, DE 19716 USA. EM kniel@udel.edu RI Hirneisen, Kirsten/B-6949-2013 NR 50 TC 29 Z9 29 U1 1 U2 60 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAY PY 2012 VL 9 IS 5 BP 396 EP 405 DI 10.1089/fpd.2011.1044 PG 10 WC Food Science & Technology SC Food Science & Technology GA 936MZ UT WOS:000303595800003 PM 22458717 ER PT J AU Pittenger, LG Frye, JG McNerney, V Reeves, J Haro, J Fedorka-Cray, PJ Harrison, MA Englen, MD AF Pittenger, Lauren G. Frye, Jonathan G. McNerney, Victoria Reeves, Jaxk Haro, Jovita Fedorka-Cray, Paula J. Harrison, Mark A. Englen, Mark D. TI Analysis of Campylobacter jejuni Whole-Genome DNA Microarrays: Significance of Prophage and Hypervariable Regions for Discriminating Isolates SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID NATURAL TRANSFORMATION; STRAINS; REVEALS; DIVERSITY; SEQUENCE; IDENTIFICATION; PATHOGENS; BACTERIA; DESIGN; GENES AB Campylobacter is a leading cause of foodborne illness in humans, and improving our understanding of the epidemiology of this organism is essential. The objective of this study was to identify the genes that discriminate isolates of C. jejuni by analysis with whole-genome DNA microarrays. Statistical analyses of whole-genome data from 95 geographically diverse cattle, chicken, and human C. jejuni isolates identified 142 most significant variable genes. Of this total, 125 (88%) belonged to genomic prophage and hypervariable regions. The significance of genomic prophage and hypervariable regions in determining C. jejuni isolate genomic diversity is emphasized by these results. These genes will be useful as biomarkers and components of genotyping systems for C. jejuni to improve our understanding of the epidemiology and population genetics of this major foodborne pathogen. C1 [Englen, Mark D.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. [Pittenger, Lauren G.] Booz Allen Hamilton Inc, Atlanta, GA USA. [McNerney, Victoria; Reeves, Jaxk] Univ Georgia, Dept Stat, Athens, GA 30602 USA. [Harrison, Mark A.] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. RP Englen, MD (reprint author), ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Richard B Russell Agr Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM mark.englen@ars.usda.gov RI Frye, Jonathan/I-6382-2013 OI Frye, Jonathan/0000-0002-8500-3395 FU United States Department of Agriculture, Agricultural Research Service CRIS [6612-32000-035, 5325-42000-0450] FX We thank Sandra House of the Bacterial Epidemiology and Antimicrobial Resistance Research Unit excellent for technical assistance. This work was supported by the United States Department of Agriculture, Agricultural Research Service CRIS (projects 6612-32000-035 and 5325-42000-0450. NR 33 TC 2 Z9 2 U1 2 U2 3 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD MAY PY 2012 VL 9 IS 5 BP 473 EP 479 DI 10.1089/fpd.2011.1048 PG 7 WC Food Science & Technology SC Food Science & Technology GA 936MZ UT WOS:000303595800013 PM 22506962 ER PT J AU Kendall, A McPherson, EG AF Kendall, Alissa McPherson, E. Gregory TI A life cycle greenhouse gas inventory of a tree production system SO INTERNATIONAL JOURNAL OF LIFE CYCLE ASSESSMENT LA English DT Article DE Carbon footprint; Climate change; Nursery; Tree planting; Tree production; Urban forestry ID ENERGY USE; OPERATIONS; EMISSIONS; IMPACTS AB This study provides a detailed, process-based life cycle greenhouse gas (GHG) inventory of an ornamental tree production system for urban forestry. The success of large-scale tree planting initiatives for climate protection depends on projects being net sinks for CO2 over their entire life cycle. However, previous assessments of urban tree planting initiatives have not accounted for the inputs required for tree production in nurseries, which include greenhouse systems, irrigation, and fertilization. A GHG inventory of nursery operations for tree production is a necessary step to assess the life cycle benefits or drawbacks of large-scale tree planting activities. Using surveys, interviews, and life cycle inventory databases, we developed a process-based life cycle inventory of GHG emissions for a large nursery operation in California, USA. The inventory demonstrated that 4.6 kg of CO2-equivalent is emitted per #5 (nominally a 5-gallon) tree, a common tree size produced by nurseries. Energy use contributed 44% of all CO2-equivalent emissions, of which electricity and propane constituted 78%. Electricity use is dominated by irrigation demands, and propane is used primarily for greenhouse heating. Material inputs constituted the next largest contributor at 36% of emissions; plastic containers contributed just over half of these emissions. Transport emissions accounted for 16% of total nursery GHG emissions. Shipping bamboo stakes from China (43%) and diesel fuel consumed by nursery delivery trucks (33%) were the largest transport emission sources. GHG emissions from the tree production life stage are 20% to 50% of mean annual CO2 storage rates based on urban tree inventories for three California cities. While considering nursery production alone is insufficient for drawing conclusions about the net climate change benefits of tree planting initiatives, the results demonstrate that nursery production emissions are modest compared with CO2 storage rates during tree life. Identifying key sources of emissions in the nursery tree production system can help operators reduce emissions by targeting so-called hot-spots. In particular, switching to renewable energy sources, capitalizing on energy and water efficiency opportunities, container light-weighting, and sourcing bamboo stakes from producers closer to the point of use are potential avenues for reduced emissions. C1 [Kendall, Alissa] Univ Calif Davis, Dept Civil & Environm Engn, Davis, CA 95616 USA. [McPherson, E. Gregory] USDA Forest Serv, Urban Ecosyst & Social Dynam Program, Pacific SW Res Stn, Davis, CA 95618 USA. RP Kendall, A (reprint author), Univ Calif Davis, Dept Civil & Environm Engn, 1 Shields Ave, Davis, CA 95616 USA. EM amkendall@ucdavis.edu RI Kendall, Alissa/E-5562-2012; OI Kendall, Alissa/0000-0003-1964-9080 FU Monrovia Nursery Company FX The authors thank Mr. John Keller, Vice President Operations, Monrovia Nursery Company, for his interest and support of this research. We are indebted to the many other individuals and companies that provided information for the study. NR 34 TC 11 Z9 13 U1 2 U2 46 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0948-3349 J9 INT J LIFE CYCLE ASS JI Int. J. Life Cycle Assess. PD MAY PY 2012 VL 17 IS 4 BP 444 EP 452 DI 10.1007/s11367-011-0339-x PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 933TE UT WOS:000303384800008 ER PT J AU Edwards, JV Castro, NJ Condon, B Costable, C Goheen, SC AF Edwards, J. Vincent Castro, Nathan J. Condon, Brian Costable, Carmen Goheen, Steven C. TI Chromatographic and Traditional Albumin Isotherms on Cellulose: A Model for Wound Protein Adsorption on Modified Cotton SO JOURNAL OF BIOMATERIALS APPLICATIONS LA English DT Article DE albumin; cotton; isotherm; wound dressings; chronic wounds ID PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMAN NEUTROPHIL ELASTASE; HUMAN-PLASMA ALBUMIN; ION-EXCHANGE; ELECTROKINETIC PROPERTIES; MEMBRANE CHROMATOGRAPHY; COMPETITIVE ADSORPTION; DRESSINGS; INTERFACES; SEPARATION AB Albumin is the most abundant protein found in healing wounds. Traditional and chromatographic protein isotherms of albumin binding on modified cotton fibers are useful in understanding albumin binding to cellulose wound dressings. An important consideration in the design of cellulosic wound dressings is adsorption and accumulation of proteins like albumin at the solid-liquid interface of the biological fluid and wound dressing fiber. To better understand the effect of fiber charge and molecular modifications in cellulose-containing fibers on the binding of serum albumin as observed in protease sequestrant dressings, albumin binding to modified cotton fibers was compared with traditional and chromatographic isotherms. Modified cotton including carboxymethylated, citrate-crosslinked, dialdehyde and phosphorylated cotton, which sequester elastase and collagenase, were compared for their albumin binding isotherms. Albumin isotherms on citrate-cellulose, cross-linked cotton demonstrated a two-fold increased binding affinity over untreated cotton. A comparison of albumin binding between traditional, solution isotherms and chromatographic isotherms on modified cellulose yielded similar equilibrium constants. Application of the binding affinity of albumin obtained in the in vitro protein isotherm to the in vivo wound dressing uptake of the protein is discussed. The chromatographic approach to assessment of albumin isotherms on modified cellulose offers a more rapid approach to evaluating protein binding on modified cellulose over traditional solution approaches. C1 [Edwards, J. Vincent; Condon, Brian; Costable, Carmen] USDA ARS, New Orleans, LA USA. [Castro, Nathan J.; Goheen, Steven C.] Battelle Mem Inst, Pacific NW Div, Richland, WA USA. RP Edwards, JV (reprint author), USDA ARS, New Orleans, LA USA. EM vince.edwards@ars.usda.gov NR 59 TC 1 Z9 1 U1 1 U2 12 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0885-3282 J9 J BIOMATER APPL JI J. Biomater. Appl. PD MAY PY 2012 VL 26 IS 8 BP 939 EP 961 DI 10.1177/0885328210390542 PG 23 WC Engineering, Biomedical; Materials Science, Biomaterials SC Engineering; Materials Science GA 937HN UT WOS:000303649700003 PM 21363875 ER PT J AU Boncristiani, H Underwood, R Schwarz, R Evans, JD Pettis, J vanEngelsdorp, D AF Boncristiani, Humberto Underwood, Robyn Schwarz, Ryan Evans, Jay D. Pettis, Jeffery vanEngelsdorp, Dennis TI Direct effect of acaricides on pathogen loads and gene expression levels in honey bees Apis mellifera SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Acaricides; Honey bee; Gene expression; Varroa; Sublethal effects ID COLONY COLLAPSE DISORDER; VARROA-JACOBSONI OUD; DEFORMED WING VIRUS; FORMIC-ACID; DESTRUCTOR; DROSOPHILA; HYMENOPTERA; INSECT; APIDAE; PESTICIDES AB The effect of using acaricides to control varroa mites has long been a concern to the beekeeping industry due to unintended negative impacts on honey bee health. Irregular ontogenesis, suppression of immune defenses, and impairment of normal behavior have been linked to pesticide use. External stressors, including parasites and the pathogens they vector, can confound studies on the effects of pesticides on the metabolism of honey bees. This is the case of Varroa destructor, a mite that negatively affects honey bee health on many levels, from direct parasitism, which diminishes honey bee productivity, to vectoring and/or activating other pathogens, including many viruses. Here we present a gene expression profile comprising genes acting on diverse metabolic levels (detoxification, immunity, and development) in a honey bee population that lacks the influence of varroa mites. We present data for hives treated with five different acaricides; Apiguard (thymol), Apistan (tau-fluvalinate), Checkmite (coumaphos), Miteaway (formic acid) and ApiVar (amitraz). The results indicate that thymol, coumaphos and formic acid are able to alter some metabolic responses. These include detoxification gene expression pathways, components of the immune system responsible for cellular response and the c-Jun amino-terminal kinase (JNK) pathway, and developmental genes. These could potentially interfere with the health of individual honey bees and entire colonies. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Underwood, Robyn; vanEngelsdorp, Dennis] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Boncristiani, Humberto; Schwarz, Ryan; Evans, Jay D.; Pettis, Jeffery] ARS, USDA, Bee Res Lab, Beltsville, MD 20705 USA. [Boncristiani, Humberto] Univ N Carolina, Dept Biol, Greensboro, NC 27403 USA. RP vanEngelsdorp, D (reprint author), Univ Maryland, Dept Entomol, 4112 Plant Sci Bldg, College Pk, MD 20742 USA. EM dennis.vanengelsdorp@gmail.com RI Evans, Jay/C-8408-2012; vanEngelsdorp, Dennis/E-7934-2010 OI Evans, Jay/0000-0002-0036-4651; FU Beltsville Bee Research Laboratory FX The authors would like to thank the entire Beltsville Bee Research Laboratory staff for their valuable support. In particular, Ms. Dawn L. Lopez provided excellent technical support and Dr. Miguel Corona gave valuable comments on the preparation of this manuscript. Additionally, we thank Gus Rouse and co-workers of Kona Queen Co and the Darcy Oishi and colleagues at the Hawaiian Department of Agriculture for great support in the field. We especially thank May Berenbaum and Gene Robinson for suggesting candidate detoxification genes important for bee health. NR 63 TC 61 Z9 63 U1 15 U2 111 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAY PY 2012 VL 58 IS 5 BP 613 EP 620 DI 10.1016/j.jinsphys.2011.12.011 PG 8 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 936WA UT WOS:000303619500003 PM 22212860 ER PT J AU Regniere, J Powell, J Bentz, B Nealis, V AF Regniere, Jacques Powell, James Bentz, Barbara Nealis, Vincent TI Effects of temperature on development, survival and reproduction of insects: Experimental design, data analysis and modeling SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Temperature; Development; Phenology; Maximum likelihood; Modeling; Population dynamics ID MOUNTAIN PINE-BEETLE; CHORISTONEURA-FUMIFERANA LEPIDOPTERA; DEPENDENT DEVELOPMENT; SPRUCE BUDWORM; CLIMATE-CHANGE; PHYSIOLOGICAL TIME; METABOLIC THEORY; LIFE-CYCLES; PHENOLOGY; SIMULATION AB The developmental response of insects to temperature is important in understanding the ecology of insect life histories. Temperature-dependent phenology models permit examination of the impacts of temperature on the geographical distributions, population dynamics and management of insects. The measurement of insect developmental, survival and reproductive responses to temperature poses practical challenges because of their modality, variability among individuals and high mortality near the lower and upper threshold temperatures. We address this challenge with an integrated approach to the design of experiments and analysis of data based on maximum likelihood. This approach expands, simplifies and unifies the analysis of laboratory data parameterizing the thermal responses of insects in particular and poikilotherms in general. This approach allows the use of censored observations (records of surviving individuals that have not completed development after a certain time) and accommodates observations from temperature transfer treatments in which individuals pass only a portion of their development at an extreme (near-threshold) temperature and are then placed in optimal conditions to complete their development with a higher rate of survival. Results obtained from this approach are directly applicable to individual-based modeling of insect development, survival and reproduction with respect to temperature. This approach makes possible the development of process-based phenology models that are based on optimal use of available information, and will aid in the development of powerful tools for analyzing eruptive insect population behavior and response to changing climatic conditions. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved. C1 [Regniere, Jacques] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Stn Ste Foy, Quebec City, PQ G1V 4C7, Canada. [Powell, James] Utah State Univ, Dept Math & Stat, Logan, UT 84322 USA. [Bentz, Barbara] US Forest Serv, Rocky Mt Res Stn, Logan, UT 84321 USA. [Nealis, Vincent] Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, Victoria, BC V8Z 1M5, Canada. RP Regniere, J (reprint author), Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Stn Ste Foy, POB 10380, Quebec City, PQ G1V 4C7, Canada. EM Jacques.Regniere@NRCan.gc.ca; jim.powell@usu.edu; bbentz@fs.fed.us; vince.nealis@nrcan.gc.ca FU USDA Forest Service (Rocky Mountain Research Station); Canadian Forest Service; Alberta Sustainable Development Department; Ontario Ministry of Natural Resources; Manitoba Ministry of Natural Resources; Saskatchewan Environment FX We thank Matt Hansen, Lynn Rasmussen and Jim Vandygriff for their assistance with mountain pine beetle phloem sandwich construction and phenology data collection. The USDA Forest Service (Rocky Mountain Research Station), Canadian Forest Service, Alberta Sustainable Development Department, the Ontario and Manitoba Ministries of Natural Resources, and Saskatchewan Environment provided funding for this research. NR 67 TC 58 Z9 60 U1 14 U2 140 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAY PY 2012 VL 58 IS 5 BP 634 EP 647 DI 10.1016/j.jinsphys.2012.01.010 PG 14 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 936WA UT WOS:000303619500006 PM 22310012 ER PT J AU Chang, CL Coudron, TA Goodman, CL Stanley, DW AF Chang, C. L. Coudron, T. A. Goodman, C. L. Stanley, D. W. TI Larval dietary wheat germ oil influences age-specific protein expression in adults of the oriental fruit fly SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Wheat germ oil; Fruit fly diet; Bactrocera dorsalis; Proteomics ID GENE-EXPRESSION; MELON FLY; TEPHRITIDAE; DIPTERA; PEROXIREDOXIN; SYSTEM; CELL AB Changes in essential dietary components alter global gene expression patterns in animals. We reported on a proteomics study designed to identify molecular markers of deficiencies in culture media developed for the oriental fruit fly, Bactrocera dorsalis. In that study, we found significant changes in expression of 70 proteins in adults of larvae reared on media lacking wheat germ oil (WGO), compared to media supplemented with WGO. Of these, a gene encoding an insect chitin-binding protein was expressed at about 120-fold higher levels in adult males reared on media supplemented with WGO. We inferred it may be feasible to develop the gene as a molecular marker of dietary lipid deficiency. The work was focused, however, on analysis of 11 day old adults. We have no information on expression of the chitin-binding protein, nor on any other proteins at other adult ages. In this paper we address the idea that the whole animal proteome changes dynamically with age. We reared separate groups of fruit fly larvae on media with and without WGO supplementation and analyzed protein expression in adult males and females age 0, 4, 8 and 12 days old using 2D electrophoresis. Gel densitometry revealed significant increases (by >2-fold) and decreases (by >50%) in expression levels of 29 proteins in females and 10 in males. We identified these proteins by mass spectrometry on MALDI TOF/TOF and bioinformatic analyses of the protein sequences. Two proteins, peroxiredoxin (26-fold increase) and vitellogenin 1 (15-fold increase) increased in expression in day 8 females. The key finding is that most changes in protein expression occurred in day 8 females. We infer that the fruit fly proteome changes with adult age. The natural changes in proteome with adult age is a crucial aspect of developing these and other proteins into molecular markers of lipid deficiency in fruit flies and possibly other insect species. Published by Elsevier Ltd. C1 [Chang, C. L.] ARS, USDA, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Coudron, T. A.; Goodman, C. L.; Stanley, D. W.] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Chang, CL (reprint author), ARS, USDA, US Pacific Basin Agr Res Ctr, 69 Nowelo St, Hilo, HI 96720 USA. EM stella.chang@ars.usda.gov NR 28 TC 2 Z9 2 U1 1 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD MAY PY 2012 VL 58 IS 5 BP 690 EP 698 DI 10.1016/j.jinsphys.2012.02.001 PG 9 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 936WA UT WOS:000303619500011 PM 22326456 ER PT J AU Harrison, RL Popham, HJR Breitenbach, JE Rowley, DL AF Harrison, Robert L. Popham, Holly J. R. Breitenbach, Jonathan E. Rowley, Daniel L. TI Genetic variation and virulence of Autographa californica multiple nucleopolyhedrovirus and Trichoplusia ni single nucleopolyhedrovirus isolates SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Autographa californica; Baculovirus; Nucleopolyhedrovirus; Trichoplusia ni; AcMNPV; TnSNPV ID NUCLEAR POLYHEDROSIS-VIRUS; BOMBYX-MORI-NUCLEOPOLYHEDROVIRUS; GENOMIC SEQUENCE-ANALYSIS; RESTRICTION-ENDONUCLEASE ANALYSIS; PLUTELLA-XYLOSTELLA; ANAGRAPHA-FALCIFERA; GALLERIA-MELLONELLA; SPODOPTERA-LITURA; DIAMONDBACK MOTH; RACHIPLUSIA-OU AB To determine the genetic diversity within the baculovirus species Autographa calfornica multiple nucleopolyhedrovirus (AcMNPV; Baculoviridae: Alphabaculovirus), a PCR-based method was used to identify and classify baculoviruses found in virus samples from the lepidopteran host species A. californica, Autographa gamma, Trichoplusia ni, Rachiplusia ou, Anagrapha falcifera, Galleria mellonella, and Heliothis virescens. Alignment and phylogenetic inference from partial nucleotide sequences of three highly conserved genes (lef-8, lef-9, and polh) indicated that 45 of 74 samples contained isolates of AcMNPV, while six samples contained isolates of Rachiplusia ou multiple nucleopolyhedrovirus strain R1 (RoMNPV-R1) and 25 samples contained isolates of the species Trichoplusia ni single nucleopolyhedrovirus (TnSNPV; Alphabaculovirus). One sample from A. californica contained a previously undescribed NPV related to alphabaculoviruses of the armyworm genus Spodoptera. Data from PCR and sequence analysis of the ie-2 gene and a region containing ORF ac86 in samples from the AcMNPV and RoMNPV clades indicated a distinct group of viruses, mostly from G. mellonella, that are characterized by an unusual ie-2 gene previously found in the strain Plutella xylostella multiple nucleopolyhedrovirus CL3 (PlxyMNPV-CL3) and a large deletion within ac86 previously described in the AcMNPV isolate 1.2 and PlxyMNPV-CL3. PCR and sequence analysis of baculovirus repeated ORF (bro) genes revealed that the bro gene ac2 was split into two separate bro genes in some samples from the AcMNPV clade. Comparison of sequences in this region suggests that ac2 was formed by a deletion that fused the two novel bra genes together. In bioassays of a selection of isolates against T. ni, significant differences were observed in the insecticidal properties of individual isolates, but no trends were observed among the AcMNPV, TnSNPV, or RoMNPV groups of isolates. This study expands on what we know about the variation of AcMNPV, AcMNPV-like and TnSNPV viruses, provides novel information on the distinct groups in which AcMNPV isolates occur, and contributes to data useful for the registration, evaluation, and improvement of AcMNPV. AcMNPV-like, and TnSNPV isolates as biological control agents. Published by Elsevier Inc. C1 [Harrison, Robert L.] ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, USDA,BARC W, Beltsville, MD 20705 USA. [Popham, Holly J. R.; Breitenbach, Jonathan E.] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. RP Harrison, RL (reprint author), ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, USDA,BARC W, Bldg 011A,Room 214,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Robert.L.Harrison@ars.usda.gov; Holly.Popham@ars.usda.gov; Jonathan.Breitenbach@ars.usda.gov; Daniel.Rowley@ars.usda.gov RI Harrison, Robert/I-4707-2014; OI Harrison, Robert/0000-0002-8348-3874 NR 70 TC 7 Z9 7 U1 1 U2 15 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD MAY PY 2012 VL 110 IS 1 BP 33 EP 47 DI 10.1016/j.jip.2012.02.002 PG 15 WC Zoology SC Zoology GA 937DL UT WOS:000303639100005 PM 22349146 ER PT J AU Kakani, R Fowler, J Haq, AU Murphy, EJ Rosenberger, TA Berhow, M Bailey, CA AF Kakani, Radhika Fowler, Justin Haq, Akram-Ul Murphy, Eric J. Rosenberger, Thad A. Berhow, Mark Bailey, Christopher. A. TI Camelina Meal Increases Egg n-3 Fatty Acid Content Without Altering Quality or Production in Laying Hens SO LIPIDS LA English DT Article DE Laying hens; Camelina meal; Egg quality; n-3 Fatty acids; Egg enrichment ID FISH-OIL; SATIVA; OMEGA-3-FATTY-ACIDS; YOLK; PERFORMANCE; NUTRITION; STORAGE; DIETS; FLAX AB Camelina sativa is an oilseed plant rich in n-3 and n-6 fatty acids and extruding the seeds results in high protein meal (similar to similar to 40%) containing high levels of n-3 fatty acids. In this study, we examined the effects of feeding extruded defatted camelina meal to commercial laying hens, measuring egg production, quality, and fatty acid composition. Lohmann White Leghorn hens (29 weeks old) were randomly allocated to three dietary treatment groups (n = 25 per group) and data was collected over a 12 week production period. All the treatment groups were fed a corn soy based experimental diet containing 0% (control), 5, or 10% extruded camelina meal. We found no significant differences in percent hen-day egg production and feed consumed per dozen eggs. Egg shell strength was significantly higher in both camelina groups compared to the controls. Egg total n-3 fatty acid content increased 1.9- and 2.7-fold in 5 and 10% camelina groups respectively relative to the control. A similar increase in DHA content also occurred. Further camelina meal did not alter glucosinolate levels and no detectable glucosinolates or metabolic product isothiocyanates were found in the eggs from either the 5 or 10% camelina groups. These results indicate that camelina meal is a viable dietary source of n-3 fatty acids for poultry and its dietary inclusion results in eggs enriched with n-3 fatty acids. C1 [Kakani, Radhika; Fowler, Justin; Haq, Akram-Ul; Bailey, Christopher. A.] Texas A&M Univ, Dept Poultry Sci, Kleberg Ctr, College Stn, TX 77843 USA. [Murphy, Eric J.; Rosenberger, Thad A.] Univ N Dakota, Sch Med & Hlth Sci, Dept Pharmacol Physiol & Therapeut, Grand Forks, ND 58201 USA. [Murphy, Eric J.] Agragen LLC, Cincinnati, OH USA. [Berhow, Mark] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. RP Kakani, R (reprint author), Texas A&M Univ, Dept Poultry Sci, Kleberg Ctr, Room 101, College Stn, TX 77843 USA. EM kakani@neo.tamu.edu FU Great Plain Oil and Exploration FX This study was funded by Great Plain Oil and Exploration. Camelina meal used in this study was supplied by Great Plain Oil and Exploration. NR 38 TC 13 Z9 13 U1 2 U2 32 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 J9 LIPIDS JI Lipids PD MAY PY 2012 VL 47 IS 5 BP 519 EP 526 DI 10.1007/s11745-012-3656-3 PG 8 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA 933IP UT WOS:000303354400008 PM 22302480 ER PT J AU Ometto, L Ross, KG Shoemaker, D Keller, L AF Ometto, Lino Ross, Kenneth G. Shoemaker, DeWayne Keller, Laurent TI Disruption of gene expression in hybrids of the fire ants Solenopsis invicta and Solenopsis richteri SO MOLECULAR ECOLOGY LA English DT Article DE ants; caste; gene expression; hybrids; Solenopsis invicta; Solenopsis richteri ID POGONOMYRMEX HARVESTER ANTS; GENOME-WIDE PATTERNS; DROSOPHILA-SIMULANS; PHENOTYPIC PLASTICITY; CASTE DETERMINATION; HALDANES RULE; MOLECULAR PHYLOGENY; POPULATION-GENETICS; SEQUENCE VARIATION; GROUP HYMENOPTERA AB Transcriptome analysis is a powerful tool for unveiling the distribution and magnitude of genetic incompatibilities between hybridizing taxa. The nature of such incompatibilities is closely associated with the evolutionary histories of the parental species and may differ across tissues and between the sexes. In eusocial insects, the presence of castes that experience divergent selection regimes may result in additional distinct patterns of caste-specific hybrid incompatibilities. We analysed levels of expression of >14 000 genes in two life stages of each caste in the fire ants Solenopsis invicta and Solenopsis richteri and in their hybrids. We found strong contributions of both developmental stage and caste to gene expression patterns. In contrast, variability in expression was only weakly associated with taxonomic identity, with hybrid scores falling between those of the two parental species. Hybrid incompatibilities were surprisingly modest, with only 32 genes being mis-expressed, indicating low levels of disruption in gene regulation in hybrids; males and workers each mis-expressed at least seven times as many genes as queens. Interestingly, homologues of many of the mis-expressed genes have been implicated in behavioural variation in Drosophila melanogaster. General expression profiles of hybrids consistently were more similar to those of S. richteri than S. invicta, presumably because S. richteri trans-regulatory elements tend to be dominant and/or because there is an overall bias in the genetic composition of the hybrids towards S. richteri. Altogether, our results suggest that selection acting on each caste may contribute differently to interspecific divergence and speciation in this group of ants. C1 [Ometto, Lino; Keller, Laurent] Univ Lausanne, Dept Ecol & Evolut, CH-1015 Lausanne, Switzerland. [Ross, Kenneth G.] Univ Georgia, Dept Entomol, Athens, GA 30602 USA. [Shoemaker, DeWayne] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. RP Ometto, L (reprint author), Fdn Edmund Mach, Dept Biodivers & Mol Ecol, Res & Innovat Ctr, I-38010 San Michele All Adige, TN, Italy. EM lino.ometto@fmach.it RI Ometto, Lino/C-3609-2008; OI Ometto, Lino/0000-0002-2679-625X; keller, laurent/0000-0002-5046-9953 FU Roche Research Foundation; Swiss NSF; ERC FX We thank Christine La Mendola and the Lausanne Genomic Technologies Facility for technical support, the Keller laboratory for helpful discussions and two reviewers for helpful comments on the manuscript. This work was supported by grants from the Roche Research Foundation (to L.O.) and Swiss NSF (L. K.) as well as an ERC advanced grant (L.K.). NR 87 TC 3 Z9 3 U1 5 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD MAY PY 2012 VL 21 IS 10 BP 2488 EP 2501 DI 10.1111/j.1365-294X.2012.05544.x PG 14 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 933UI UT WOS:000303388300016 PM 22548254 ER PT J AU Qiu, YJ Xi, J Du, LQ Suttle, JC Poovaiah, BW AF Qiu, Yongjian Xi, Jing Du, Liqun Suttle, Jeffrey C. Poovaiah, B. W. TI Coupling calcium/calmodulin-mediated signaling and herbivore-induced plant response through calmodulin-binding transcription factor AtSR1/CAMTA3 SO PLANT MOLECULAR BIOLOGY LA English DT Article DE Calcium; Calmodulin; Herbivore; Jasmonates; AtSR1/CAMTA3 ID DEPENDENT PROTEIN-KINASE; SALICYLIC-ACID; JASMONIC ACID; CROSS-TALK; ARABIDOPSIS-THALIANA; INDUCED EXPRESSION; STRESS RESPONSES; GENE-EXPRESSION; ABIOTIC STRESS; TOMATO LEAVES AB Calcium/calmodulin (Ca2+/CaM) has long been considered a crucial component in wound signaling pathway. However, very few Ca2+/CaM-binding proteins have been identified which regulate plant responses to herbivore attack/wounding stress. We have reported earlier that a family of Ca2+/CaM-binding transcription factors designated as AtSRs (also known as AtCAMTAs) can respond differentially to wounding stress. Further studies revealed that AtSR1/CAMTA3 is a negative regulator of plant defense, and Ca2+/CaM-binding to AtSR1 is indispensable for the suppression of salicylic acid (SA) accumulation and disease resistance. Here we report that Ca2+/CaM-binding is also critical for AtSR1-mediated herbivore-induced wound response. Interestingly, atsr1 mutant plants are more susceptible to herbivore attack than wild-type plants. Complementation of atsr1 mutant plants by overexpressing wild-type AtSR1 protein can effectively restore plant resistance to herbivore attack. However, when mutants of AtSR1 with impaired CaM-binding ability were overexpressed in atsr1 mutant plants, plant resistance to herbivore attack was not restored, suggesting a key role for Ca2+/CaM-binding in wound signaling. Furthermore, it was observed that elevated SA levels in atsr1 mutant plants have a negative impact on both basal and induced biosynthesis of jasmonates (JA). These results revealed that Ca2+/CaM-mediated signaling regulates plant response to herbivore attack/wounding by modulating the SA-JA crosstalk through AtSR1. C1 [Qiu, Yongjian; Xi, Jing; Du, Liqun; Poovaiah, B. W.] Washington State Univ, Dept Hort, Pullman, WA 99164 USA. [Du, Liqun] Hangzhou Normal Univ, Coll Life & Environm Sci, Hangzhou 310036, Zhejiang, Peoples R China. [Suttle, Jeffrey C.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. RP Poovaiah, BW (reprint author), Washington State Univ, Dept Hort, POB 646414, Pullman, WA 99164 USA. EM poovaiah@wsu.edu FU National Science Foundation [1021344]; National Research Initiative from the U.S. Department of Agriculture, National Institute of Food and Agriculture [2008-35100-04566] FX This research was supported by National Science Foundation grant no. 1021344 and the National Research Initiative Competitive Grant no. 2008-35100-04566 from the U.S. Department of Agriculture, National Institute of Food and Agriculture. We thank Linda L. Huckle of the U.S. Department of Agriculture, Agricultural Research Service, Northern Crop Science Laboratory, Fargo, ND for her help with SA, JA and JA-Ile quantification, and Lorie Mochel, Washington State University, for her help with preparing the manuscript. NR 57 TC 26 Z9 31 U1 3 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4412 J9 PLANT MOL BIOL JI Plant Mol.Biol. PD MAY PY 2012 VL 79 IS 1-2 BP 89 EP 99 DI 10.1007/s11103-012-9896-z PG 11 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 934AH UT WOS:000303410400008 PM 22371088 ER PT J AU Nam, S Condon, BD White, RH Zhao, Q Yao, F Cintron, MS AF Nam, Sunghyun Condon, Brian D. White, Robert H. Zhao, Qi Yao, Fei Cintron, Michael Santiago TI Effect of urea additive on the thermal decomposition kinetics of flame retardant greige cotton nonwoven fabric SO POLYMER DEGRADATION AND STABILITY LA English DT Article DE Cotton; Flame; Nitrogen phosphorus synergy; Kinetics; Thermogravimetry; NMR ID THERMOGRAVIMETRIC ANALYSIS; DEGRADATION BEHAVIORS; ACTIVATION-ENERGY; EPOXY-RESINS; MODEL-FREE; FT-IR; CELLULOSE; PYROLYSIS; PHOSPHATE; NITROGEN AB Urea is well known to have a synergistic action with phosphorus-based flame retardants (FRs) in enhancing the FR performance of cellulosic materials, but the effect of urea on the thermal decomposition kinetics has not been thoroughly studied. In this study, the activation energy (E-a) for the thermal decomposition of greige cotton nonwoven fabrics treated with various amounts of urea at fixed contents of diammonium phosphate (DAP), was measured using the Kissinger, Friedman, and Flynn-Wall-Ozawa methods. The three methods produced consistent results revealing a dual function of urea additive in the kinetics depending on the concentration. Those functions were correlated with the synergistic FR action of urea. Up to a certain concentration, the addition of urea raised the overall E-a. The steeply increasing trend of E-a observed in the low conversions indicates that urea enhanced the multiple reactions of DAP, which were confirmed by P-31 MAS NMR and ATR-FTIR. Higher concentrations of urea additive, however, significantly lowered the E-a values for both the DAP reactions and the decomposition of cellulose. As evidenced by a slight reduction in char yield, the decrease in E-a suggests that the excess of urea acted to facilitate the diffusion of volatiles and heat transfer in the cotton structure, resulting in catalyzing the decomposition of cellulose at low temperatures. The latter function was predominant when the greater synergistic FR action of urea was achieved. Published by Elsevier Ltd. C1 [Nam, Sunghyun; Condon, Brian D.; Cintron, Michael Santiago] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [White, Robert H.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Zhao, Qi] Tulane Univ, Dept Chem, New Orleans, LA 70118 USA. [Yao, Fei] Louisiana State Univ, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. RP Condon, BD (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM brian.condon@ars.usda.gov NR 49 TC 5 Z9 5 U1 0 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0141-3910 EI 1873-2321 J9 POLYM DEGRAD STABIL JI Polym. Degrad. Stabil. PD MAY PY 2012 VL 97 IS 5 BP 738 EP 746 DI 10.1016/j.polymdegradstab.2012.02.008 PG 9 WC Polymer Science SC Polymer Science GA 932QT UT WOS:000303306300007 ER PT J AU Vick, BD Neal, BA AF Vick, Brian D. Neal, Byron A. TI Analysis of off-grid hybrid wind turbine/solar PV water pumping systems SO SOLAR ENERGY LA English DT Article DE Wind; Solar; Hybrid; Water pumping; PV; Wind turbine ID POWER-GENERATION SYSTEM AB While many remote water pumping systems exist (e.g. mechanical windmills, solar photovoltaic, wind-electric, diesel powered), few combine both the wind and solar energy resources to possibly improve the reliability and the performance of the system. In this paper, off-grid wind turbine (WT) and solar photovoltaic (PV) array water pumping systems were analyzed individually and combined as a hybrid system. The objectives were to determine: (1) advantages or disadvantages of using a hybrid system over using a WT or a solar PV array alone; (2) if the WT or solar PV array interfered with the output of the other; and (3) which hybrid system was the most efficient for the location. The WT used in the analysis was rated at 900 W alternating current (AC). There were three different solar PV arrays analyzed, and they were rated at 320, 480, and 640 W direct current (DC). A rectifier converted the 3-phase variable voltage AC output from the WT to DC before combining it with the solar PV array DC output. The combined renewable energies powered a single helical pump. The independent variable used in the hybrid WT/PV array analysis was in units of W/m(2). The peak pump efficiency of the hybrid systems at Bush land, TX occurred for the 900 W WT combined with the 640 W PV array. The peak pump efficiencies at a 75 m pumping depth of the hybrid systems were: 47% (WT/320 W PV array), 51% (WT/480 W PV array), and 55% (WT/640 W PV array). Interference occurred between the WT and the different PV arrays (likely due to voltage mismatch between WT and PV array), but the least interference occurred for the WT/320 W PV array. This hybrid system pumped 28% more water during the greatest water demand month than the WT and PV systems would have pumped individually. An additional controller with a buck/boost converter is discussed at end of paper for improvement of the hybrid WT/PV array water pumping system. Published by Elsevier Ltd. C1 [Vick, Brian D.; Neal, Byron A.] ARS, USDA, Bushland, TX 79012 USA. RP Vick, BD (reprint author), ARS, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM brian.vick@ars.usda.gov NR 29 TC 17 Z9 19 U1 0 U2 32 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-092X J9 SOL ENERGY JI Sol. Energy PD MAY PY 2012 VL 86 IS 5 BP 1197 EP 1207 DI 10.1016/j.solener.2012.01.012 PG 11 WC Energy & Fuels SC Energy & Fuels GA 932QF UT WOS:000303304900008 ER PT J AU Beecher, BS Carter, AH See, DR AF Beecher, Brian S. Carter, Arron H. See, Deven R. TI Genetic mapping of new seed-expressed polyphenol oxidase genes in wheat (Triticum aestivum L.) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID QUANTITATIVE TRAIT LOCI; COMMON WHEAT; GRAIN HARDNESS; DURUM-WHEAT; PPO GENES; KERNELS; IDENTIFICATION; PUROINDOLINE; COMPONENTS; CULTIVARS AB Polyphenol oxidase (PPO) enzymatic activity is a major cause in time-dependent discoloration in wheat dough products. The PPO-A1 and PPO-D1 genes have been shown to contribute to wheat kernel PPO activity. Recently a novel PPO gene family consisting of the PPO-A2, PPO-B2, and PPO-D2 genes has been identified and shown to be expressed in wheat kernels. In this study, the sequences of these five kernel PPO genes were determined for the spring wheat cultivars Louise and Penawawa. The two cultivars were found to be polymorphic at each of the PPO loci. Three novel alleles were isolated from Louise. The Louise X Penawawa mapping population was used to genetically map all five PPO genes. All map to the long arm of homeologous group 2 chromosomes. PPO-A2 was found to be located 8.9 cM proximal to PPO-A1 on the long arm of chromosome 2A. Similarly, PPO-D1 and PPO-D2 were separated by 10.7 cM on the long arm of chromosome 2D. PPO-B2 mapped to the long arm of chromosome 2B and was the site of a novel QTL for polyphenol oxidase activity. Five other PPO QTL were identified in this study. One QTL corresponds to the previously described PPO-D1 locus, one QTL corresponds to the PPO-D2 locus, whereas the remaining three are located on chromosome 2B. C1 [Beecher, Brian S.; Carter, Arron H.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [See, Deven R.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Beecher, Brian S.; See, Deven R.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Beecher, BS (reprint author), Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. EM bbeecher@wsu.edu FU National Research Initiative Competitive Grants CAP from USDA National Institute of Food and Agriculture [2011-68002-30029] FX This project was supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. All experiments presented here comply with current US law. NR 39 TC 16 Z9 16 U1 1 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD MAY PY 2012 VL 124 IS 8 BP 1463 EP 1473 DI 10.1007/s00122-012-1801-2 PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 934HM UT WOS:000303434800008 PM 22311372 ER PT J AU Meiman, PJ Davis, NR Brummer, JE Ippolito, JA AF Meiman, P. J. Davis, N. R. Brummer, J. E. Ippolito, J. A. TI Riparian Shrub Metal Concentrations and Growth in Amended Fluvial Mine Tailings SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Riparian restoration; Heavy metal toxicity; Aboveground metal concentrations; Belowground metal concentrations; Salix; Alnus; Betula; Dasiphora; Cornus ID CONTAMINATED SOILS; NITROGEN-FIXATION; BETULA-PENDULA; CADMIUM; PB; ACCUMULATION; COPPER; SALIX; CD; CU AB Fluvial mine tailing deposition has caused extensive riparian damage throughout the western USA. Willows are often used for fluvial mine tailing revegetation, but some accumulate excessive metal concentrations potentially detrimental to browsers. This greenhouse experiment evaluated growth and metal accumulation of Geyer willow (Salix geyeriana Andersson), Drummond's willow (Salix drummondiana Barratt ex Hook.), diamondleaf willow (Salix planifolia Pursh), Bebb willow (Salix bebbiana Sarg.), thinleaf alder [Alnus incana (L.) Moench spp. tenuifolia (Nutt.) Breitung], water birch (Betula occidentalis Hook.), red-osier dogwood (Cornus sericea L. spp. sericea), and shrubby cinquefoil [(Dasiphora fruticosa (L.) Rydb. ssp. floribunda (Pursh) Kartesz)]. Bare-root shrubs were grown in tailings collected from three acidic, metal-contaminated (i.e., Cd, Cu, Pb, and Zn) fluvial deposits near Leadville, Colorado, USA. Tailings were amended with only lime to raise the soil pH to 7 s.u., or with lime and composted biosolids (224 Mg ha(-1)). All shrubs survived in the amended tailings; composted biosolids had little effect on plant biomass. Aboveground and belowground biomass increased during the 2-month greenhouse study by 3-9 and 1.5-5 times initial values, respectively. Most shrubs accumulated Pb and Cu in roots, and belowground Pb concentrations in all shrubs were significantly reduced by the addition of composted biosolids. Compared to other species, alder and cinquefoil accumulated Pb in aboveground growth, and concentrations exceeded animal toxicity thresholds, but these shrubs normally comprise a small proportion of animal diets. Dogwood, alder, and cinquefoil contained low Cd concentrations in aboveground new growth, whereas Bebb and Geyer willow contained zootoxic concentrations. Dogwood, alder, and cinquefoil are three good candidates for mine tailing revegetation, especially in fluvial deposits with elevated Cd concentrations. C1 [Meiman, P. J.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. [Davis, N. R.] US EPA, Denver, CO USA. [Brummer, J. E.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Ippolito, J. A.] ARS, USDA, NW Irrigat & Soils Res Lab, Kimberly, ID USA. RP Meiman, PJ (reprint author), Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. EM pmeiman@warnercnr.colostate.edu FU Colorado State University Agricultural Experiment Station FX This research was funded by the Colorado State University Agricultural Experiment Station, with Dr. Lee Sommers as the Director. NR 68 TC 2 Z9 2 U1 1 U2 25 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD MAY PY 2012 VL 223 IS 4 BP 1815 EP 1828 DI 10.1007/s11270-011-0986-3 PG 14 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA 933TO UT WOS:000303385800031 ER PT J AU Weaver, MA Boyette, CD Hoagland, RE AF Weaver, Mark A. Boyette, C. Douglas Hoagland, Robert E. TI Bioherbicidal activity from washed spores of Myrothecium verrucaria SO WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY LA English DT Article DE Bioherbicide; Sicklepod; Cassia obtusifolia; kudzu; Pueraria lobata; Roridin; Verrucarin ID KUDZU PUERARIA-LOBATA; BIOLOGICAL-CONTROL; ISOLATE; STRAIN; AGENT AB The fungal plant pathogen, Myrothecium verrucaria, is highly virulent to several important weed species and has potential utility as a bioherbicide. However the production of macrocyclic trichothecene mycotoxins by this fungus presents significant safety concerns. It was discovered that trichothecenes are removed from M. verrucaria spores by repeated washes with water. These washed spores retained bioherbicidal efficacy against kudzu when tested in field trials and on sicklepod when tested under greenhouse conditions. Changes in the growth medium combined with washing spores with water resulted in greater than 95% reduction in roridin A and verrucarin A. Washing spores reduced trichothecene concentrations in spore preparations with no significant effect on plant biomass reduction, thus demonstrating the possibility of M. verrucaria formulations with improved safety to researchers, producers and applicators. C1 [Weaver, Mark A.; Boyette, C. Douglas; Hoagland, Robert E.] ARS, USDA, Stoneville, MS 38776 USA. RP Weaver, MA (reprint author), ARS, USDA, Stoneville, MS 38776 USA. EM mark.weaver@ars.usda.gov OI Weaver, Mark/0000-0001-9020-7264 NR 19 TC 3 Z9 6 U1 0 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0959-3993 J9 WORLD J MICROB BIOT JI World J. Microbiol. Biotechnol. PD MAY PY 2012 VL 28 IS 5 BP 1941 EP 1946 DI 10.1007/s11274-011-0996-8 PG 6 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 933TP UT WOS:000303385900009 PM 22806015 ER PT J AU Bailey, RL Holden, J Dwyer, JT AF Bailey, Regan L. Holden, Joanne Dwyer, Johanna T. TI Intakes of copper in nutrition surveys are falsely high Reply SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Letter C1 [Bailey, Regan L.; Dwyer, Johanna T.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Holden, Joanne] ARS, Nutrient Data Lab, USDA, Beltsville, MD USA. RP Bailey, RL (reprint author), NIH, Off Dietary Supplements, 6100 Execut Blvd,Room 3B01, Bethesda, MD 20892 USA. EM baileyr@mail.nih.gov NR 2 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD MAY PY 2012 VL 95 IS 5 BP 1294 EP 1294 DI 10.3945/ajcn.112.035451 PG 1 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 930LK UT WOS:000303140700044 ER PT J AU Azimzadeh, AM Tareen, J Dawson, H Burdorf, L Rybak, E Zhang, T Ayares, D Pierson, RN AF Azimzadeh, A. M. Tareen, J. Dawson, H. Burdorf, L. Rybak, E. Zhang, T. Ayares, D. Pierson, R. N. TI Expression of Inflammation and Coagulation Genes during GalTKO. hCD46 Xenogenic Lung Perfusion SO AMERICAN JOURNAL OF TRANSPLANTATION LA English DT Meeting Abstract CT American Transplant Congress CY MAY, 2012 CL Boston, MA C1 [Azimzadeh, A. M.; Tareen, J.; Burdorf, L.; Rybak, E.; Zhang, T.; Pierson, R. N.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. [Dawson, H.] USDA, Beltsville, MD 20705 USA. [Ayares, D.] Revivicor, Blacksburg, VA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1600-6135 J9 AM J TRANSPLANT JI Am. J. Transplant. PD MAY PY 2012 VL 12 SU 3 SI SI MA 864 BP 282 EP 282 PG 1 WC Surgery; Transplantation SC Surgery; Transplantation GA 931QX UT WOS:000303235502085 ER PT J AU Mavrodi, OV Mavrodi, DV Parejko, JA Thomashow, LS Weller, DM AF Mavrodi, Olga V. Mavrodi, Dmitri V. Parejko, James A. Thomashow, Linda S. Weller, David M. TI Irrigation Differentially Impacts Populations of Indigenous Antibiotic-Producing Pseudomonas spp. in the Rhizosphere of Wheat SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID AUREOFACIENS STRAIN 30-84; FLUORESCENT PSEUDOMONADS; PHENAZINE ANTIBIOTICS; SOIL SUPPRESSIVENESS; BIOLOGICAL-CONTROL; PACIFIC-NORTHWEST; BIOFILM FORMATION; PUTIDA BIOFILMS; FUSARIUM-WILT; TAKE-ALL AB This work determined the impact of irrigation on the seasonal dynamics of populations of Pseudomonas spp. producing the antibiotics phenazine-1-carboxylic acid (Phz(+)) and 2,4-diacetylphloroglucinol (Phl(+)) in the rhizosphere of wheat grown in the low-precipitation zone (150 to 300 mm annually) of the Columbia Plateau of the Inland Pacific Northwest. Population sizes and plant colonization frequencies of Phz(+) and Phl(+) Pseudomonas spp. were determined in winter and spring wheat collected during the growing seasons from 2008 to 2009 from selected commercial dryland and irrigated fields in central Washington State. Only Phz(+) bacteria were detected on dryland winter wheat, with populations ranging from 4.8 to 6.3 log CFU g(-1) of root and rhizosphere colonization frequencies of 67 to 100%. The ranges of population densities of Phl(+) and Phz(+) Pseudomonas spp. recovered from wheat grown under irrigation were similar, but 58 to 100% of root systems were colonized by Phl(+) bacteria whereas only 8 to 50% of plants harbored Phz(+) bacteria. In addition, Phz(+) Pseudomonas spp. were abundant in the rhizosphere of native plant species growing in nonirrigated areas adjacent to the sampled dryland wheat fields. This is the first report that documents the impact of irrigation on indigenous populations of two closely related groups of antibiotic-producing pseudomonads that coinhabit the rhizosphere of an economically important cereal crop. These results demonstrate how crop management practices can influence indigenous populations of antibiotic-producing pseudomonads with the capacity to suppress soilborne diseases of wheat. C1 [Thomashow, Linda S.; Weller, David M.] ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Mavrodi, Olga V.; Mavrodi, Dmitri V.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Parejko, James A.] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. RP Weller, DM (reprint author), ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. EM wellerd@wsu.edu RI Mavrodi, Dmitri/J-2745-2013 NR 36 TC 7 Z9 8 U1 2 U2 20 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD MAY PY 2012 VL 78 IS 9 BP 3214 EP 3220 DI 10.1128/AEM.07968-11 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 926BY UT WOS:000302807500021 PM 22389379 ER PT J AU Murthy, GS Johnston, DB Rausch, KD Tumbleson, ME Singh, V AF Murthy, Ganti S. Johnston, David B. Rausch, Kent D. Tumbleson, M. E. Singh, Vijay TI A simultaneous saccharification and fermentation model for dynamic growth environments SO BIOPROCESS AND BIOSYSTEMS ENGINEERING LA English DT Article DE Saccharomyces cerevisiae; Cybernetic model; Environmental effects; Convergence ID LIMITED CHEMOSTAT CULTURES; SACCHAROMYCES-CEREVISIAE; LACTIC-ACID; ACETIC-ACID; ETHANOL; YEAST; STARCH; BATCH; TEMPERATURE; KINETICS AB Many mathematical models by researchers have been formulated for Saccharomyces cerevisiae which is the common yeast strain used in modern distilleries. A cybernetic model that can account for varying concentrations of glucose, ethanol and organic acids on yeast cell growth dynamics does not exist. A cybernetic model, consisting of 4 reactions and 11 metabolites simulating yeast metabolism, was developed. The effects of variables such as temperature, pH, organic acids, initial inoculum levels and initial glucose concentration were incorporated into the model. Further, substrate and product inhibitions were included. The model simulations over a range of variables agreed with hypothesized trends and to observations from other researchers. Simulations converged to expected results and exhibited continuity in predictions for all ranges of variables simulated. The cybernetic model did not exhibit instability under any conditions simulated. C1 [Murthy, Ganti S.; Rausch, Kent D.; Tumbleson, M. E.; Singh, Vijay] Univ Illinois, Dept Agr & Biol Engn, Urbana, IL 61801 USA. [Johnston, David B.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Singh, V (reprint author), Univ Illinois, Dept Agr & Biol Engn, 360G AESB,1304 W Penn Ave, Urbana, IL 61801 USA. EM murthy@engr.orst.edu; vsingh@illinois.edu RI Murthy, Ganti /P-5272-2015 NR 26 TC 7 Z9 9 U1 1 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1615-7591 J9 BIOPROC BIOSYST ENG JI Bioprocess. Biosyst. Eng. PD MAY PY 2012 VL 35 IS 4 BP 519 EP 534 DI 10.1007/s00449-011-0625-9 PG 16 WC Biotechnology & Applied Microbiology; Engineering, Chemical SC Biotechnology & Applied Microbiology; Engineering GA 929IG UT WOS:000303053900005 PM 21987306 ER PT J AU Fan, XY Upadhyaya, B Wu, LM Koh, C Santin-Duran, M Pittaluga, S Uzel, G Kleiner, D Williams, E Ma, CA Bodansky, A Oliveira, JB Edmonds, P Hornung, R Wong, DW Fayer, R Fleisher, T Heller, T Prussin, C Jain, A AF Fan, Xiying Upadhyaya, Bhaskar Wu, Liming Koh, Christopher Santin-Duran, Monica Pittaluga, Stefania Uzel, Gulbu Kleiner, David Williams, Ester Ma, Chi A. Bodansky, Aaron Oliveira, Joao B. Edmonds, Pamela Hornung, Ronald Wong, Duane W. Fayer, Ronald Fleisher, Tom Heller, Theo Prussin, Calman Jain, Ashish TI CD40 agonist antibody mediated improvement of chronic Cryptosporidium infection in patients with X-linked hyper IgM syndrome SO CLINICAL IMMUNOLOGY LA English DT Article DE X-linked hyper-IgM syndrome; CD40 ligand; CP-870,893; CD40-R internalization ID DENDRITIC CELLS; CD40-CD40L INTERACTIONS; IMMUNOLOGICAL FEATURES; T-CELLS; PHASE-I; LIGAND; MICE; ACTIVATION; IMMUNODEFICIENCY; DEFICIENCY AB X-linked hyper-IgM syndrome (XHM) is a combined immune deficiency disorder caused by mutations in CD40 ligand. We tested CP-870,893, a human CD40 agonist monoclonal antibody, in the treatment of two XHM patients with biliary Cryptosporidiosis. CP-870,893 activated B cells and APCs in vitro, restoring class switch recombination in XHM B cells and inducing cytokine secretion by monocytes. CP-870,893 infusions were well tolerated and showed significant activity in vivo, decreasing leukocyte concentration in peripheral blood. Although specific antibody responses were lacking, frequent dosing in one subject primed T cells to secrete IFN-g and suppressed oocyst shedding in the stool. Nevertheless, relapse occurred after discontinuation of therapy. The CD40 receptor was rapidly internalized following binding with CP-870,893, potentially explaining the limited capacity of CP-870,893 to mediate immune reconstitution. This study demonstrates that CP-870,893 suppressed oocysts shedding in XHM patients with biliary cryptosporidiosis. The continued study of CD40 agonists in XHM is warranted. Published by Elsevier Inc. C1 [Fan, Xiying; Wu, Liming; Ma, Chi A.; Bodansky, Aaron; Edmonds, Pamela; Jain, Ashish] NIAID, Lab Host Def, NIH, Bethesda, MD 20892 USA. [Upadhyaya, Bhaskar; Prussin, Calman] NIAID, Lab Allerg Dis, NIH, Bethesda, MD 20892 USA. [Uzel, Gulbu] NIAID, Lab Clin Infect Dis, NIH, Bethesda, MD 20892 USA. [Koh, Christopher; Heller, Theo] NIDDK, Liver Dis Branch, NIH, Bethesda, MD USA. [Williams, Ester; Oliveira, Joao B.; Fleisher, Tom] NIH, Dept Lab Med, Bethesda, MD 20892 USA. [Pittaluga, Stefania; Kleiner, David] NCI, Pathol Lab, NIH, Bethesda, MD 20892 USA. [Wong, Duane W.] Arizona Allergy Associates, Phoenix, AZ USA. [Hornung, Ronald] NCI, SAIC Frederick Inc, Clin Serv Program, Frederick, MD 21701 USA. [Santin-Duran, Monica; Fayer, Ronald] USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Jain, A (reprint author), NIAID, Lab Host Def, NIH, 10 Ctr Dr,Rm 5W 3950, Bethesda, MD 20892 USA. EM ajain@nih.gov OI Oliveira, Joao/0000-0001-9388-8173; Prussin, Calman/0000-0002-3917-3326; Fan, Xiying/0000-0003-3090-381X; Kleiner, David/0000-0003-3442-4453 FU National Cancer Institute, National Institutes of Health [N01-CO-12400]; NIAID/NIH FX The project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400, and supported by the intramural program of NIAID/NIH. NR 38 TC 8 Z9 9 U1 1 U2 6 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1521-6616 J9 CLIN IMMUNOL JI Clin. Immunol. PD MAY PY 2012 VL 143 IS 2 BP 152 EP 161 DI 10.1016/j.clim.2012.01.014 PG 10 WC Immunology SC Immunology GA 932KV UT WOS:000303290900006 PM 22459705 ER PT J AU Franks, SJ Wheeler, GS Goodnight, C AF Franks, Steven J. Wheeler, Gregory S. Goodnight, Charles TI GENETIC VARIATION AND EVOLUTION OF SECONDARY COMPOUNDS IN NATIVE AND INTRODUCED POPULATIONS OF THE INVASIVE PLANT MELALEUCA QUINQUENERVIA SO EVOLUTION LA English DT Article DE Biological control; EICA hypothesis; evolutionary constraints; G matrix; herbivory; natural selection; phenotypic evolution ID BIOLOGICAL-CONTROL AGENT; INCREASED COMPETITIVE ABILITY; COVARIANCE MATRICES; NATURAL-SELECTION; OXYOPS-VITIOSA; FIELD COLONIZATION; WILD RADISH; CAV. BLAKE; DEFENSE; FLORIDA AB We examined multivariate evolution of 20 leaf terpenoids in the invasive plant Melaleuca quinquenervia in a common garden experiment. Although most compounds, including 1,8-Cineole and Viridiflorol, were reduced in home compared with invaded range genotypes, consistent with an evolutionary decrease in defense, one compound (E-Nerolidol) was greater in invaded than home range genotypes. Nerolidol was negatively genetically correlated with Cineole and Viridiflorol, and the increase in this compound in the new range may have been driven by this negative correlation. There was positive selection on all three focal compounds, and a loss of genetic variation in introduced range genotypes. Selection skewers analysis predicted an increase in Cineole and Viridiflorol and a decrease or no change in Nerolidol, in direct contrast to the observed changes in the new range. This discrepancy could be due to differences in patterns of selection, genetic correlations, or the herbivore communities in the home versus introduced ranges. Although evolutionary changes in most compounds were consistent with the evolution of increased competitive ability hypothesis, changes in other compounds as well as selection patterns were not, indicating that it is important to understand selection and the nature of genetic correlations to predict evolutionary change in invasive species. C1 [Franks, Steven J.] Fordham Univ, Dept Biol Sci, Bronx, NY 10458 USA. [Wheeler, Gregory S.] ARS, Invas Plant Res Lab, USDA, Ft Lauderdale, FL 33314 USA. [Goodnight, Charles] Univ Vermont, Dept Biol Sci, Burlington, VT 05405 USA. RP Franks, SJ (reprint author), Fordham Univ, Dept Biol Sci, Larkin Hall,441 E Fordham Rd, Bronx, NY 10458 USA. EM franks@fordham.edu FU USDA/ARS TAME Melaleuca Area; Fordham University research office; Fordham College at Rose Hill Dean's office FX We thank S. Gonzales, P. Pratt, J. Scheidegger, and M. Stetson for help with field work and K. MacDonald for lab work. P. Pratt provided logistical support for the experiment. M. Purcell (CSIRO) provided seeds from Australia, and F. Allen Dray (USDA) provided seeds from Florida. A. Hoffmann, Associate Editor K. Donohue, and two reviewers gave useful comments on the manuscript. Financial support was provided by the USDA/ARS TAME Melaleuca Area Wide Project and by grants to S. Franks from the Fordham University research office and the Fordham College at Rose Hill Dean's office. NR 74 TC 11 Z9 12 U1 4 U2 61 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0014-3820 J9 EVOLUTION JI Evolution PD MAY PY 2012 VL 66 IS 5 BP 1398 EP 1412 DI 10.1111/j.1558-5646.2011.01524.x PG 15 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 929FS UT WOS:000303047300009 PM 22519780 ER PT J AU Palik, B Martin, M Zenner, E Blinn, C Kolka, R AF Palik, Brian Martin, Michelle Zenner, Eric Blinn, Charles Kolka, Randy TI Overstory and regeneration dynamics in riparian management zones of northern Minnesota forested watersheds SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Riparian management zone; Plant communities; Harvesting impacts; Regeneration ID BUFFER STRIPS; MICROCLIMATIC GRADIENTS; RESIDUAL OVERSTORY; EDGE INFLUENCE; SMALL STREAMS; COAST RANGE; NEW-ZEALAND; DISTURBANCE; VEGETATION; OREGON AB We quantified tree regeneration under different riparian management zone (RMZ) treatments along first-order streams in Minnesota, USA. A primary objective for long-term management of RMZs in the study region is to maintain some tree cover and promote establishment of later successional tree species and conifers. We also compared regeneration response to contrasting harvesting systems that differed in expected soil disturbance and impact on residual vegetation. Riparian treatments included: (1) full control (no cutting in RMZ (60 m-wide in all treatments) or adjacent upland stand), (2) riparian control (RMZ uncut; adjacent upland stand clearcut); and partially-harvested RMZs (RMZ basal area reduced from 29 to 13 m(2)/ha, adjacent upland stand clearcut) and using (3) cut-to-length or (4) tree-length harvesting. Nine years after treatment, basal area of the full control had not changed appreciably, while basal area of the riparian control had declined by 28% and basal area of the two partial-harvest treatments had decreased by 54%; reductions were due to blowdown of residual trees. Total regeneration density was stable over time in the full control and riparian control, but increased substantially in the two RMZ treatments. Regeneration response was driven by early successional species, mostly Populus tremuloides and Betula papyrifera, and shrubs. Responses were similar between the two harvesting systems. Our results show that regeneration does increase with partial harvesting of RMZs, that the response is mostly due to early succession, shorter-lived deciduous species, and that harvest systems that differ in expected site and vegetation impact elicit similar results. A lack of significant increases in conifers and longer-lived trees in the RMZs following treatment, and the substantial increases in shrubs which may inhibit establishment of these species, suggests a need for more active approaches to establish these species, for example through under planting and competition control. Published by Elsevier B.V. C1 [Palik, Brian; Kolka, Randy] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. [Martin, Michelle] Minnesota Dept Nat Resources, Div Forestry, St Paul, MN 55155 USA. [Zenner, Eric] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. [Blinn, Charles] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. RP Palik, B (reprint author), US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. EM bpalik@fs.fed.us; Michelle.Martin@mn.state.us; eric.zenner@psu.edu; cblinn@umn.edu; rkolka@fs.fed.us OI Blinn, Charlie/0000-0003-1981-5421 FU Minnesota Forest Resources Council; National Council of the Paper Industry for Air and Stream Improvement; Boise Cascade Corporation; USDA Forest Service Northern Research Station; University of Minnesota's Department of Forest Resources; University of Minnesota Extension; Minnesota Agricultural Experiment Station [MN 42-042, MN-42-022] FX We thank Jim Marshall, John Hanson, and Cheryl Adams of UPM Blandin Paper Company for logistic assistance and valuable insight into project design and implementation. Thanks to Jim Robl for study site layout, extensive fieldwork, and initial data summarization. The Minnesota Forest Resources Council, the National Council of the Paper Industry for Air and Stream Improvement, Boise Cascade Corporation, the USDA Forest Service Northern Research Station, University of Minnesota's Department of Forest Resources, the University of Minnesota Extension, and the Minnesota Agricultural Experiment Station under Projects MN 42-042 and MN-42-022 supported this research. NR 62 TC 6 Z9 6 U1 0 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2012 VL 271 BP 1 EP 9 DI 10.1016/j.foreco.2012.01.045 PG 9 WC Forestry SC Forestry GA 928ZE UT WOS:000303030200001 ER PT J AU Hicke, JA Johnson, MC Jane, LHD Preisler, HK AF Hicke, Jeffrey A. Johnson, Morris C. Jane, L. Hayes D. Preisler, Haiganoush K. TI Effects of bark beetle-caused tree mortality on wildfire SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Wildfire; Bark beetles; Fire behavior; Fuels; Forest disturbances ID SUB-ALPINE FORESTS; WESTERN UNITED-STATES; MOUNTAIN PINE; LODGEPOLE PINE; FIRE SEVERITY; CONIFEROUS FORESTS; CROWN FIRE; ROCKY-MOUNTAINS; KENAI PENINSULA; NATIONAL-PARK AB Millions of trees killed by bark beetles in western North America have raised concerns about subsequent wildfire, but studies have reported a range of conclusions, often seemingly contradictory, about effects on fuels and wildfire. In this study, we reviewed and synthesized the published literature on modifications to fuels and fire characteristics following beetle-caused tree mortality. We found 39 studies addressing this topic with a variety of methods including fuels measurements, fire behavior simulations, an experiment, and observations of fire occurrence, severity, or frequency. From these publications, we developed a conceptual framework describing expected changes of fuels and fire behavior. Some characteristics of fuels and fire are enhanced following outbreaks and others are unchanged or diminished, with time since outbreak a key factor influencing changes. We also quantified areas of higher and lower confidence in our framework based on the number of studies addressing a particular area as well as agreement among studies. The published literature agrees about responses in many conditions, including fuels measurements and changes in stands with longer times since outbreak, and so we assigned higher confidence to our conceptual framework for these conditions. Disagreement or gaps in knowledge exist in several conditions, particularly in early postoutbreak phases and crown fire behavior responses, leading to low confidence in our framework in these areas and highlighting the need for future research. Our findings resolved some of the controversy about effects of bark beetles on fire through more specificity about time since outbreak and fuels or fire characteristic. Recognition of the type of study question was also important in resolving controversy: some publications assessed whether beetle-caused tree mortality caused differences relative to unattacked locations, whereas other publications assessed differences relative to other drivers of wildfire such as climate. However, some disagreement among studies remained. Given the large areas of recent bark beetle and wildfire disturbances and expected effects of climate change, land and fire managers need more confidence in key areas when making decisions about treatments to reduce future fire hazard and when fighting fires. (C) 2012 Elsevier B.V. All rights reserved. C1 [Hicke, Jeffrey A.] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. [Hicke, Jeffrey A.] US Forest Serv, Western Wildland Environm Threat Assessment Ctr, USDA, Prineville, OR 97754 USA. [Johnson, Morris C.] US Forest Serv, Pacific Wildland Fire Sci Lab, USDA, Seattle, WA 98103 USA. [Jane, L. Hayes D.; Preisler, Haiganoush K.] US Forest Serv, Pacific SW Res Stn, USDA, Albany, CA 94710 USA. RP Hicke, JA (reprint author), Univ Idaho, Dept Geog, POB 443021, Moscow, ID 83844 USA. EM jhicke@uidaho.edu RI Hicke, Jeff/M-9677-2013 FU USDA Forest Service Western Wildland Environmental Threat Assessment Center; Pacific Northwest Research Station; Pacific Southwest Research Station FX This work was supported by the USDA Forest Service Western Wildland Environmental Threat Assessment Center, Pacific Northwest Research Station, and Pacific Southwest Research Station. We are grateful for informative conversations with members of the fire management community. Thanks to Martin Simard for sharing his fuels measurements. We also thank many colleagues who have provided feedback that improved this study, particularly David L. Peterson, Don McKenzie, Nancy Grulke, and Terry Shaw, as well as several anonymous reviewers, whose comments improved the manuscript. NR 70 TC 92 Z9 95 U1 15 U2 92 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2012 VL 271 BP 81 EP 90 DI 10.1016/j.foreco.2012.02.005 PG 10 WC Forestry SC Forestry GA 928ZE UT WOS:000303030200010 ER PT J AU Perry, RW Rudolph, DC Thill, RE AF Perry, Roger W. Rudolph, D. Craig Thill, Ronald E. TI Effects of short-rotation controlled burning on amphibians and reptiles in pine woodlands SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Amphibians; Arkansas; Burning; Fire; Lizards; Snakes ID PRESCRIBED FIRE; PLETHODONTID SALAMANDERS; SPECIES-DIVERSITY; FLORIDA SANDHILL; NATIONAL-PARK; SMALL MAMMALS; RESPONSES; FOREST; HERPETOFAUNA; RESTORATION AB Fire is being used increasingly as a forest management tool throughout North America, but its effects on reptiles and amphibians in many ecosystems are unclear. Open woodlands with understories dominated by herbaceous vegetation benefit many wildlife species, but maintaining these woodlands requires frequent burning. Although many studies have compared herpetofaunal responses in burned forests to unburned forests, fewer studies have examined changes in the herpetofaunal community during the interval between short-rotation prescribed burns. We examined changes in habitat and relative abundance of reptiles and amphibians each year within a 3-year burn cycle in nine restored pine woodlands of western Arkansas, USA. Overall numbers of reptiles did not change among the three burn years; however, capture rates for one snake species (southern black racer [Coluber constrictor priapus]) and three lizards differed among the three post-burn years. Overall capture rate for anurans and all amphibians combined was greatest the first year after burning, mostly because captures of dwarf American toads (Bufo americanus charlessmithi) were substantially greater in stands the first year after burning. Salamander captures were infrequent. Capture rates of most reptile and amphibian species declined over the 3-year sampling period. Only minor changes in capture rate occurred among the 36 species we evaluated during the intervening years between frequent burns. Capture rates for only two species were lower the first year after fire. Although not all herpetofaunal species benefited from frequent fire, most species appeared to tolerate the frequent burning necessary for maintaining open pine woodlands in the Ouachita Mountains. Published by Elsevier B.V. C1 [Perry, Roger W.] US Forest Serv, So Res Stn, USDA, Hot Springs, AR 71902 USA. [Rudolph, D. Craig; Thill, Ronald E.] US Forest Serv, So Res Stn, USDA, Nacogdoches, TX 75965 USA. RP Perry, RW (reprint author), US Forest Serv, So Res Stn, USDA, POB 1270, Hot Springs, AR 71902 USA. EM rperry03@fs.fed.us FU Southern Research Station; ONF FX We thank R. Buford, H. Williamson, T. Tanner, and students from Stephen F. Austin State University, Arkansas Tech University, and the University of Arkansas at Monticello for field assistance. We thank N. Koerth, D. Saenz, and K. Greenberg for helpful reviews. We also thank W. Montague and personnel of the Poteau-Cold Springs Ranger District of the ONF for their assistance locating sites and ensuring burns were completed on schedule. Funding was provided by the Southern Research Station and the ONF through the efforts of J. Guldin and L. Hedrick. NR 68 TC 6 Z9 6 U1 5 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2012 VL 271 BP 124 EP 131 DI 10.1016/j.foreco.2012.02.004 PG 8 WC Forestry SC Forestry GA 928ZE UT WOS:000303030200015 ER PT J AU Wilson, BT Lister, AJ Riemann, RI AF Wilson, B. Tyler Lister, Andrew J. Riemann, Rachel I. TI A nearest-neighbor imputation approach to mapping tree species over large areas using forest inventory plots and moderate resolution raster data SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Nearest-neighbor imputation; Canonical correspondence analysis; MODIS; Vegetation phenology; Forest inventory; Species distribution ID CANONICAL CORRESPONDENCE-ANALYSIS; CONTERMINOUS UNITED-STATES; DIRECT GRADIENT ANALYSIS; DISTRIBUTION MODELS; LANDSCAPE-SCALE; COASTAL OREGON; CLIMATE-CHANGE; NDVI DATA; VEGETATION; TERRAIN AB The paper describes an efficient approach for mapping multiple individual tree species over large spatial domains. The method integrates vegetation phenology derived from MODIS imagery and raster data describing relevant environmental parameters with extensive field plot data of tree species basal area to create maps of tree species abundance and distribution at a 250-m pixel size for the entire eastern contiguous United States. The approach uses the modeling techniques of k-nearest neighbors and canonical correspondence analysis, where model predictions are calculated using a weighting of nearest neighbors based on proximity in a feature space derived from the model. The approach also utilizes a stratification derived from the 2001 National Land-Cover Database tree canopy cover layer. Data pre-processing is also described, which includes the use of Fourier series transformation for data reduction and characterizing seasonal vegetation phenology patterns that are apparent in the MODIS imagery. A suite of assessment procedures is applied to each of the modeled dataset presented. These indicate high accuracies, at the scales of assessments used, for total live-tree basal area per hectare and for many of the most common tree species found in the study area. The end result is an approach that enables the mapping of individual tree species distributions, while retaining much of the species covariance found on the forest inventory plots, at a level of spatial detail approaching that required for many regional management and planning applications. The proposed approach has the potential for operational application for simultaneously mapping the distribution and abundance of numerous common tree species across large spatial domains. Published by Elsevier B.V. C1 [Wilson, B. Tyler; Lister, Andrew J.; Riemann, Rachel I.] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. RP Wilson, BT (reprint author), US Forest Serv, No Res Stn, USDA, 1992 Folwell Ave, St Paul, MN 55108 USA. EM barrywilson@fs.fed.us NR 63 TC 41 Z9 44 U1 6 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD MAY 1 PY 2012 VL 271 BP 182 EP 198 DI 10.1016/j.foreco.2012.02.002 PG 17 WC Forestry SC Forestry GA 928ZE UT WOS:000303030200021 ER PT J AU Bradford, JB D'Amato, AW AF Bradford, John B. D'Amato, Anthony W. TI Recognizing trade-offs in multi-objective land management SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID PINUS-RESINOSA STANDS; CLIMATE-CHANGE; FOREST MANAGEMENT; DECISION-MAKING; UNITED-STATES; DIVERSITY; BIODIVERSITY; GROWTH; CONSEQUENCES; MITIGATION AB As natural resource management and conservation goals expand and evolve, practitioners and policy makers are increasingly seeking options that optimize benefits among multiple, often contradictory objectives. Here, we describe a simple approach for quantifying the consequences of alternative management options in terms of benefits and trade-offs among multiple objectives. We examine two long-term forest management experiments that span several decades of stand (forest tree community) development and identify substantial trade-offs among carbon cycling and ecological complexity objectives. In addition to providing improved understanding of the long-term consequences of various management options, the results of these experiments show that positive benefits resulting from some management options are often associated with large trade-offs among individual objectives. The approach to understanding benefits and trade-offs presented here provides a simple yet flexible framework for quantitatively assessing the consequences of different management options. C1 [Bradford, John B.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN USA. [D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. RP Bradford, JB (reprint author), US Geol Survey, SW Biol Sci Ctr, Flagstaff, AZ 86001 USA. EM jbradford@usgs.gov RI Bradford, John/E-5545-2011 FU US Forest Service Center; NASA [CARBON/04-0225-0191, CARBON/04-0120-0011]; Minnesota Agricultural Experiment Station FX The authors are indebted to the numerous USDA Forest Service scientists and technicians who established and maintained the long-term experiments used in this study, particularly B Palik, P Zehngraff, Z Zasada, R Buckman, J Benzie, J Zasada, R Barse, J Elioff, T Strong, GC Erdmann, T Crow, C Kern, and D Kastendick. JBB was supported by funding from the US Forest Service Center for Research on Ecosystem Change and NASA Carbon Cycle Science research grants CARBON/04-0225-0191 and CARBON/04-0120-0011. Funding for AWD was provided by the Minnesota Agricultural Experiment Station. NR 33 TC 20 Z9 24 U1 7 U2 56 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD MAY PY 2012 VL 10 IS 4 BP 210 EP 216 DI 10.1890/110031 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 934EC UT WOS:000303425200019 ER PT J AU Jackson, TJ Bindlish, R Cosh, MH Zhao, TJ Starks, PJ Bosch, DD Seyfried, M Moran, MS Goodrich, DC Kerr, YH Leroux, D AF Jackson, Thomas J. Bindlish, Rajat Cosh, Michael H. Zhao, Tianjie Starks, Patrick J. Bosch, David D. Seyfried, Mark Moran, Mary Susan Goodrich, David C. Kerr, Yann H. Leroux, Delphine TI Validation of Soil Moisture and Ocean Salinity (SMOS) Soil Moisture Over Watershed Networks in the U.S. SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Advanced Microwave Scanning Radiometer (AMSR-E); passive microwave; Soil Moisture and Ocean Salinity (SMOS); soil moisture; validation ID RADIOMETRY; STABILITY; ALGORITHM AB Estimation of soil moisture at large scale has been performed using several satellite-based passive microwave sensors and a variety of retrieval methods over the past two decades. The most recent source of soil moisture is the European Space Agency Soil Moisture and Ocean Salinity (SMOS) mission. A thorough validation must be conducted to insure product quality that will, in turn, support the widespread utilization of the data. This is especially important since SMOS utilizes a new sensor technology and is the first passive L-band system in routine operation. In this paper, we contribute to the validation of SMOS using a set of four in situ soil moisture networks located in the U. S. These ground-based observations are combined with retrievals based on another satellite sensor, the Advanced Microwave Scanning Radiometer (AMSR-E). The watershed sites are highly reliable and address scaling with replicate sampling. Results of the validation analysis indicate that the SMOS soil moisture estimates are approaching the level of performance anticipated, based on comparisons with the in situ data and AMSR-E retrievals. The overall root-mean-square error of the SMOS soil moisture estimates is 0.043 m(3)/m(3) for the watershed networks (ascending). There are bias issues at some sites that need to be addressed, as well as some outlier responses. Additional statistical metrics were also considered. Analyses indicated that active or recent rainfall can contribute to interpretation problems when assessing algorithm performance, which is related to the contributing depth of the satellite sensor. Using a precipitation flag can improve the performance. An investigation of the vegetation optical depth (tau) retrievals provided by the SMOS algorithm indicated that, for the watershed sites, these are not a reliable source of information about the vegetation canopy. The SMOS algorithms will continue to be refined as feedback from validation is evaluated, and it is expected that the SMOS estimates will improve. C1 [Jackson, Thomas J.; Bindlish, Rajat; Cosh, Michael H.; Zhao, Tianjie] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Starks, Patrick J.] ARS, Grazinglands Res Lab, USDA, El Reno, OK 73036 USA. [Bosch, David D.] ARS, SE Watershed Res Ctr, USDA, Tifton, GA 31793 USA. [Seyfried, Mark] ARS, NW Watershed Res Ctr, USDA, Boise, ID 83712 USA. [Moran, Mary Susan; Goodrich, David C.] ARS, SW Watershed Res Ctr, USDA, Tucson, AZ 85719 USA. [Kerr, Yann H.; Leroux, Delphine] Ctr Etud Spatiales BIOsphere CESBIO, F-31401 Toulouse, France. RP Jackson, TJ (reprint author), ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. EM tom.jackson@ars.usda.gov; rajat.bindlish@ars.usda.gov; michael.cosh@ars.usda.gov; tianjie.zhao@ars.usda.gov; patrick.starks@ars.usda.gov; david.bosch@ars.usda.gov; mark.seyfried@ars.usda.gov; susan.moran@ars.usda.gov; dave.goodrich@ars.usda.gov; yann.kerr@cesbio.cnes.fr; delphine.leroux@cesbio.cnes.fr RI Goodrich, David/B-1763-2009; Cosh, MIchael/A-8858-2015; OI Goodrich, David/0000-0001-7735-1448; Cosh, MIchael/0000-0003-4776-1918; Leroux, Delphine/0000-0003-1688-021X FU ESA FX The SMOS data were provided through the ESA supported SMOS Validation and Retrieval Team program. NR 30 TC 137 Z9 139 U1 6 U2 59 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAY PY 2012 VL 50 IS 5 SI SI BP 1530 EP 1543 DI 10.1109/TGRS.2011.2168533 PN 1 PG 14 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 931GU UT WOS:000303205200016 ER PT J AU Rowlandson, TL Hornbuckle, BK Bramer, LM Patton, JC Logsdon, SD AF Rowlandson, Tracy L. Hornbuckle, Brian K. Bramer, Lisa M. Patton, Jason C. Logsdon, Sally D. TI Comparisons of Evening and Morning SMOS Passes Over the Midwest United States SO IEEE TRANSACTIONS ON GEOSCIENCE AND REMOTE SENSING LA English DT Article DE Brightness temperature; soil moisture; Soil Moisture and Ocean Salinity (SMOS) ID SOIL-WATER CONTENT; DOMAIN REFLECTOMETRY TDR; BAND MICROWAVE EMISSION; CROP FIELDS; 1.4 GHZ; VEGETATION; MOISTURE; MODEL; INTERCEPTION; RADIOMETRY AB This study investigates differences in the soil moisture product and brightness temperatures between 6 P.M. and 6 A.M. local solar time Soil Moisture Ocean Salinity (SMOS) passes for a region in the Midwest United States. This region has uniform land cover, consisting largely of maize and soybean row crops. The comparison was restricted to periods with no rainfall. There were 19 days available for analysis of the soil moisture product. It was found that there was a significant difference in the soil moisture product for all 19 days, with lower soil moisture for most mornings. The difference between the soil moisture products on some days exceeded the allowable error of 0.04 m(3) m(-3). In-situ and model results indicate that there should be virtually no change in soil moisture between the evening and morning. In order to investigate this discrepancy, measured brightness temperature was converted to a polarization index (PI), and evening and morning values were compared. Investigation of the measured brightness temperature was limited to five days where a large range in incidence angles was available. Large differences between evening and morning passes were found for incidence angles less than 40 degrees that could not be explained with radiative transfer theory but may be attributed to technical issues. There was also a difference in the PI values between the evening and morning passes for incidence angles greater than 40 degrees. This can be caused by a decrease in soil moisture from evening to morning or could be attributed to an increase in the volumetric water content of the vegetation. C1 [Rowlandson, Tracy L.] Iowa State Univ Sci & Technol, Dept Agron, Ames, IA 50011 USA. [Hornbuckle, Brian K.; Bramer, Lisa M.; Patton, Jason C.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Logsdon, Sally D.] ARS, USDA, Ames, IA 50010 USA. RP Rowlandson, TL (reprint author), Univ Guelph, Dept Geog, Guelph, ON N1G 2W1, Canada. EM trowlan1@alumni.iastate.edu; bkh@iastate.edu; lbramer@iastate.edu; jpatton@iastate.edu; sally.logsdon@ars.usda.gov RI Bramer, Lisa/L-9184-2016 OI Bramer, Lisa/0000-0002-8384-1926 FU National Aeronautics and Space Administration [NNX08AE48G] FX This work was supported by the National Aeronautics and Space Administration under Grant NNX08AE48G from the Terrestrial Hydrology Program. NR 36 TC 22 Z9 23 U1 0 U2 11 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 0196-2892 J9 IEEE T GEOSCI REMOTE JI IEEE Trans. Geosci. Remote Sensing PD MAY PY 2012 VL 50 IS 5 SI SI BP 1544 EP 1555 DI 10.1109/TGRS.2011.2178158 PN 1 PG 12 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 931GU UT WOS:000303205200017 ER PT J AU Whitaker, WB Parent, MA Boyd, A Richards, GP Boyd, EF AF Whitaker, W. Brian Parent, Michelle A. Boyd, Aoife Richards, Gary P. Boyd, E. Fidelma TI The Vibrio parahaemolyticus ToxRS Regulator Is Required for Stress Tolerance and Colonization in a Novel Orogastric Streptomycin-Induced Adult Murine Model SO INFECTION AND IMMUNITY LA English DT Article ID THERMOSTABLE DIRECT HEMOLYSIN; III SECRETION SYSTEM; CHOLERA-TOXIN; INTESTINAL COLONIZATION; ANTIMICROBIAL PEPTIDES; CRASSOSTREA-GIGAS; VIRULENCE FACTORS; ACCESSORY TOXINS; CLINICAL ISOLATE; EFFECTOR PROTEIN AB Vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. It contains a homologue of the toxRS operon that in V. cholerae is the key regulator of virulence gene expression. We examined a nonpolar mutation in toxRS to determine the role of these genes in V. parahaemolyticus RIMD2210633, an O3:K6 isolate, and showed that compared to the wild type, Delta toxRS was significantly more sensitive to acid, bile salts, and sodium dodecyl sulfate stresses. We demonstrated that ToxRS is a positive regulator of ompU expression, and that the complementation of Delta toxRS with ompU restores stress tolerance. Furthermore, we showed that ToxRS also regulates type III secretion system genes in chromosome I via the regulation of the leuO homologue VP0350. We examined the effect of Delta toxRS in vivo using a new orogastric adult murine model of colonization. We demonstrated that streptomycin-treated adult C57BL/6 mice experienced prolonged intestinal colonization along the entire intestinal tract by the streptomycin-resistant V. parahaemolyticus. In contrast, no colonization occurred in non-streptomycin-treated mice. A competition assay between the Delta toxRS and wild-type V. parahaemolyticus strains marked with the beta-galactosidase gene lacZ demonstrated that the Delta toxRS strain was defective in colonization compared to the wild-type strain. This defect was rescued by ectopically expressing ompU. Thus, the defect in stress tolerance and colonization in Delta toxRS is solely due to OmpU. To our knowledge, the orogastric adult murine model reported here is the first showing sustained intestinal colonization by V. parahaemolyticus. C1 [Whitaker, W. Brian; Parent, Michelle A.; Boyd, E. Fidelma] Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA. [Parent, Michelle A.] Univ Delaware, Dept Med Technol, Newark, DE USA. [Boyd, Aoife] Natl Univ Ireland, Sch Nat Sci, Discipline Microbiol, Galway, Ireland. [Richards, Gary P.] Delaware State Univ, USDA, ARS, Dover, DE USA. RP Boyd, EF (reprint author), Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA. EM fboyd@udel.edu RI Boyd, Aoife/A-4775-2011; OI Boyd, Aoife/0000-0002-4631-9792 FU United States Department of Agriculture, NRI CSREES [2008-35201-04535] FX This research was supported by funding from the United States Department of Agriculture, NRI CSREES grant 2008-35201-04535. NR 87 TC 28 Z9 33 U1 1 U2 11 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD MAY PY 2012 VL 80 IS 5 BP 1834 EP 1845 DI 10.1128/IAI.06284-11 PG 12 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 925VX UT WOS:000302791100024 PM 22392925 ER PT J AU del Cacho, E Gallego, M Lee, SH Lillehoj, HS Quilez, J Lillehoj, EP Sanchez-Acedo, C AF del Cacho, Emilio Gallego, Margarita Lee, Sung Hyen Lillehoj, Hyun Soon Quilez, Joaquin Lillehoj, Erik P. Sanchez-Acedo, Caridad TI Induction of Protective Immunity against Eimeria tenella, Eimeria maxima, and Eimeria acervulina Infections Using Dendritic Cell-Derived Exosomes SO INFECTION AND IMMUNITY LA English DT Article ID GONDII INFECTION; LEISHMANIA-MAJOR; T-CELLS; ANTIGEN; RESPONSES; COCCIDIOSIS; CHICKENS; VACCINATION; MICE; CRYPTOSPORIDIUM AB This study describes a novel immunization strategy against avian coccidiosis using exosomes derived from Eimeria parasite antigen (Ag)-loaded dendritic cells (DCs). Chicken intestinal DCs were isolated and pulsed in vitro with a mixture of sporozoite-extracted Ags from Eimeria tenella, E. maxima, and E. acervulina, and the cell-derived exosomes were isolated. Chickens were nonimmunized or immunized intramuscularly with exosomes and subsequently noninfected or coinfected with E. tenella, E. maxima, and E. acervulina oocysts. Immune parameters compared among the nonimmunized/noninfected, nonimmunized/infected, and immunized/infected groups were the numbers of cells secreting T(h)1 cytokines, T(h)2 cytokines, interleukin-16 (IL-16), and Ag-reactive antibodies in vitro and in vivo readouts of protective immunity against Eimeria infection. Cecal tonsils, Peyer's patches, and spleens of immunized and infected chickens had increased numbers of cells secreting the IL-16 and the T(h)1 cytokines IL-2 and gamma interferon, greater Ag-stimulated proliferative responses, and higher numbers of Ag-reactive IgG- and IgA-producing cells following in vitro stimulation with the sporozoite Ags compared with the nonimmunized/noninfected and nonimmunized/infected controls. In contrast, the numbers of cells secreting the T(h)2 cytokines IL-4 and IL-10 were diminished in immunized and infected chickens compared with the nonimmunized/noninfected and the nonimmunized/infected controls. Chickens immunized with Ag-loaded exosomes and infected in vivo with Eimeria oocysts had increased body weight gains, reduced feed conversion ratios, diminished fecal oocyst shedding, lessened intestinal lesion scores, and reduced mortality compared with the nonimmunized/infected controls. These results suggest that successful field vaccination against avian coccidiosis using exosomes derived from DCs incubated with Ags isolated from Eimeria species may be possible. C1 [del Cacho, Emilio; Gallego, Margarita; Quilez, Joaquin; Sanchez-Acedo, Caridad] Univ Zaragoza, Dept Anim Pathol, Fac Vet Sci, Zaragoza, Spain. [Lee, Sung Hyen; Lillehoj, Hyun Soon] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD USA. [Lillehoj, Erik P.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. RP del Cacho, E (reprint author), Univ Zaragoza, Dept Anim Pathol, Fac Vet Sci, Zaragoza, Spain. EM edelcach@unizar.es FU Research Council of Aragon, Spain [A46] FX This work was supported in part by grant A46 from the Research Council of Aragon, Spain. NR 49 TC 19 Z9 19 U1 4 U2 16 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD MAY PY 2012 VL 80 IS 5 BP 1909 EP 1916 DI 10.1128/IAI.06413-11 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 925VX UT WOS:000302791100032 PM 22354026 ER PT J AU Xie, YP Xu, SJ Hu, Y Chen, WY He, YP Shi, XM AF Xie, Yanping Xu, Shujun Hu, Yu Chen, Wanyi He, Yiping Shi, Xianming TI RAPID IDENTIFICATION AND CLASSIFICATION OF STAPHYLOCOCCUS AUREUS BY ATTENUATED TOTAL REFLECTANCE FOURIER TRANSFORM INFRARED SPECTROSCOPY SO JOURNAL OF FOOD SAFETY LA English DT Article ID FT-IR SPECTROSCOPY; RAMAN-SPECTROSCOPY; DISCRIMINATION; STRAINS; BACTERIA; MICROORGANISMS; ASSAY; TOOL; PCR; QUANTIFICATION AB Staphylococcus aureus is an important bacterium that can cause serious infections in human such as pneumonia and bacteremia. Rapid detection of this pathogen is crucial in food industries and clinical laboratories to control S. aureus food poisoning and human infections. In this study, Fourier transform infrared spectroscopy equipped with a germanium attenuated total reflection accessory was used as a novel approach to identify S. aureus. A total of 17 reference strains belonging to 4 different species and 84 clinical isolates of Staphylococcus spp. were analyzed. After the cultivation of the strains, spectral collection and data preprocessing, the S. aureus isolates were identified by a two-step discrimination procedure. An internal validation and the related external validation were performed to demonstrate the discriminatory power and the quality of the discrimination models before the discrimination analysis. In the first step, 38 S. aureus isolates were correctly classified and the others were misidentified as Staphylococcus haemolyticus by hierarchical clustering analysis model using the first derivatives from the spectral range between 1,800 and 1,050/cm. In the second step, several classification/discrimination algorithms of soft-independent modeling of class analogy, principal component regression and partial least squares regression (PLSR) were applied to build models for differentiating S. aureus and S. haemolyticus. The results showed that 57 (98.3%) strains and 4 (100%) strains of S. aureus and S. haemolyticus could be correctly identified by PLSR. C1 [Xie, Yanping; Hu, Yu; Chen, Wanyi; Shi, Xianming] Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. [Xie, Yanping; Hu, Yu; Chen, Wanyi; Shi, Xianming] Shanghai Jiao Tong Univ, Sch Agr & Biol, Bor Luh Food Safety Ctr, Shanghai 200240, Peoples R China. [He, Yiping] ARS, USDA MOST Joint Res Ctr Food Safety, USDA, Eastern Reg Res Ctr USDA ARS ERRC, Wyndmoor, PA USA. [He, Yiping] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr USDA ARS ERRC, Wyndmoor, PA USA. [Xu, Shujun] Jiangsu Prov Supervising & Testing Inst Prod Qual, Nanjing, Jiangsu, Peoples R China. RP Shi, XM (reprint author), Shanghai Jiao Tong Univ, Sch Agr & Biol, MOST USDA Joint Res Ctr Food Safety, 800 Dongchuan Rd, Shanghai 200240, Peoples R China. EM xmshi@sjtu.edu.cn RI Xu, Huanyu/C-7860-2016; Xie, Yanping/C-7950-2016 FU Ministry of Science and Technology of China [2011DFA31220, SS2012AA101001]; National Natural Science Foundation of China [30972485, U1031003]; Science and Technology Commission of Shanghai Municipality [10142201300, 10DZ0503500] FX This research was jointly supported by the Ministry of Science and Technology of China (Grant No. 2011DFA31220 and No. SS2012AA101001), National Natural Science Foundation of China (Grant No. 30972485 and No. U1031003) and the Science and Technology Commission of Shanghai Municipality (Grant No. 10142201300 and No. 10DZ0503500). The authors would like to thank the Peking University Health Science Center for sampling. NR 30 TC 2 Z9 2 U1 3 U2 19 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0149-6085 J9 J FOOD SAFETY JI J. Food Saf. PD MAY PY 2012 VL 32 IS 2 BP 176 EP 183 DI 10.1111/j.1745-4565.2012.00365.x PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 927ZP UT WOS:000302949500005 ER PT J AU Leggett, LN Tomasula, PM Van Hekken, DL Porto-Fett, ACS Shoyer, B Renye, JA Luchansky, JB Farkye, N AF Leggett, L. N. Tomasula, P. M. Van Hekken, D. L. Porto-Fett, A. C. S. Shoyer, B. Renye, J. A. Luchansky, J. B. Farkye, N. TI EFFECT OF STORAGE AT 4 AND 10C ON THE GROWTH OF LISTERIA MONOCYTOGENES IN AND ON QUESO FRESCO SO JOURNAL OF FOOD SAFETY LA English DT Article ID VACUUM-SEALED PACKAGES; MEXICAN-STYLE CHEESE; BACILLUS-CEREUS; PASTEURIZED MILK; BACTERIAL-GROWTH; SOFT CHEESE; LAG PHASE; TEMPERATURE; THURINGIENSIS; ENTEROCOCCUS AB A five-strain rifampicin-resistant Listeria monocytogenes cocktail (ca. 3.0 log(10) cfu/g) was introduced as a postpasteurization contaminant in queso fresco (QF) that was manufactured using a commercial procedure. L. monocytogenes was either inoculated into (IN) the curds before forming the cheese block or onto (ON) slices (52-66 g), individually vacuum-packed and stored at 4 and 10C. Growth was monitored for up to 35 days. Gompertz analyses showed small differences in lag time because of temperature, but growth rate and generation time were faster at 10C than at 4C. After 20 days for both the IN and the ON treatments, the maximum population density was 7.80 +/- 0.17, regardless of the storage temperature. These results indicate that QF manufacture must be conducted using Good Manufacturing Practices and under hygienic conditions, and that the use of antimicrobials and/or postprocessing interventions is necessary to prevent the presence and growth of L. monocytogenes. C1 [Leggett, L. N.; Tomasula, P. M.; Van Hekken, D. L.; Renye, J. A.] ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Porto-Fett, A. C. S.; Shoyer, B.; Luchansky, J. B.] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Farkye, N.] Calif Polytech State Univ San Luis Obispo, Dairy Prod Technol Ctr, San Luis Obispo, CA 93407 USA. RP Tomasula, PM (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM peggy.tomasula@ars.usda.gov FU USDA; USDA/ARS/ERRC; Dairy Management Inc. [58-1935-9-908] FX The authors thank Dr. John Phillips, North Atlantic Area Statistician, USDA/ARS/ERRC, Wyndmoor, PA, for the assistance with the statistics; Mr. Raymond Kwoczak for the assistance with the cheese making; and Mr. Jeff Call and Mr. Matthew Toht for the assistance with the microbiology. Latasha Leggett was supported, in part, by a USDA 1890's Institution Capacity Building Grant awarded to Delaware State University (Dover, DE) and, in part, by the USDA/ARS/ERRC. This research was funded, in part, by Dairy Management Inc. and administered by the Dairy Research Institute, Rosemont, IL, through Agreement No. 58-1935-9-908. NR 42 TC 4 Z9 4 U1 0 U2 8 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0149-6085 J9 J FOOD SAFETY JI J. Food Saf. PD MAY PY 2012 VL 32 IS 2 BP 236 EP 245 DI 10.1111/j.1745-4565.2012.00373.x PG 10 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 927ZP UT WOS:000302949500013 ER PT J AU Al Rajabi, A Booth, SL Peterson, JW Choi, SW Suttie, JW Shea, MK Miao, B Grusak, MA Fu, XY AF Al Rajabi, Ala Booth, Sarah L. Peterson, James W. Choi, Sang Woon Suttie, John W. Shea, M. Kyla Miao, Benchun Grusak, Michael A. Fu, Xueyan TI Deuterium-Labeled Phylloquinone Has Tissue-Specific Conversion to Menaquinone-4 among Fischer 344 Male Rats SO JOURNAL OF NUTRITION LA English DT Article ID VITAMIN-K; CACO-2 CELLS; APOPTOSIS; TRANSPORT; HPLC AB Phylloquinone (PK) is converted into menaquinone-4 (MK-4) via side chain removal-addition. Stable isotope use is an effective approach to identify the tissue location of this conversion, which is currently unknown. Following a 14-d PK-deficient diet, male Fischer 344 rats 18 mo; n = 15) were fed 1.6 mg deuterium-labeled PK (L-PK) per kg diet for 0 (control), 1 d (PK-1d), and 7 d (PK-7d). Both L-PK and deuterium-labeled MK-4 (L-MK-4) were detected in tissues in PK-1d and PK-7d, although the results varied. Whereas some tissues had an overall increase in MK-4 in response to L-PK, total brain, testes, and fat MK-4 concentrations did not. In contrast, L-MK-4 concentrations increased in all 3 tissues. The deuterium label was found only on the L-M K-4 naphthoquinone ring, confirming the need for side chain removal for the formation of MK-4. Labeled menadione (MD) was detected in urine and serum in PK-1d and PK-7d, confirming its role as an intermediate. A Caco-2 cell monolayer model was used to study the role of the enterocytes in the conversion process. Neither MK-4 nor MD was detected in Caco-2 cells treated with PK. However, when Caco-2 cells were treated with MD, MK-4 was formed. Similarly, MK-4 was formed in response to MD-treated 293T kidney cells, but not HuH7 liver cells. These data demonstrate that MK-4 is the predominant form of vitamin K in multiple tissues, but there appears to be a tissue-specific regulation for the conversion of PK to MK-4. J. Nutr. 142: 841-845, 2012. C1 [Al Rajabi, Ala; Booth, Sarah L.; Peterson, James W.; Choi, Sang Woon; Miao, Benchun; Fu, Xueyan] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Suttie, John W.] Univ Wisconsin, Dept Biochem, Madison, WI 53705 USA. [Shea, M. Kyla] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. [Grusak, Michael A.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Booth, SL (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM sarah.booth@tufts.edu OI AL RAJABI, ALA/0000-0003-2426-6279 FU USDA Agricultural Research Service [58-1950-7-707, 58-6250-0-008]; NIH [DK69341] FX Supported by the USDA Agricultural Research Service under Cooperative Agreements no. 58-1950-7-707 and no. 58-6250-0-008, and by the NIH (DK69341). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 21 TC 18 Z9 18 U1 1 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAY PY 2012 VL 142 IS 5 BP 841 EP 845 DI 10.3945/jn.111.155804 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 929TX UT WOS:000303089700005 PM 22437559 ER PT J AU Badaloo, AV Forrester, T Reid, M Jahoor, F AF Badaloo, Asha V. Forrester, Terrence Reid, Marvin Jahoor, Farook TI Nutritional Repletion of Children with Severe Acute Malnutrition Does Not Affect VLDL Apolipoprotein B-100 Synthesis Rate SO JOURNAL OF NUTRITION LA English DT Article ID PROTEIN-ENERGY MALNUTRITION; AMINO-ACIDS; LIVER FAT; KWASHIORKOR; HYPERTRIGLYCERIDEMIA; METABOLISM; MECHANISMS; INFECTION; TRANSPORT; SEPSIS AB VLDL apo B-100 is essential for the secretion of liver fat. It is thought that synthesis of this lipoprotein is impaired in childhood severe acute malnutrition (SAM), especially in the edematous syndromes, and that this contributes to the common occurrence of hepatic steatosis in this condition. However, to our knowledge, it has not been confirmed that VLDL apo B-100 synthesis is slower in edematous compared with nonedematous SAM and that it is impaired in the malnourished compared with the well-nourished state. Therefore, VLDL apo B-100 kinetics were measured in 2 groups of children with SAM (15 edematous and 7 nonedematous), aged 4-20 mo, at 3 stages during treatment. Measurements were done at 4 +/- 1 d postadmission, mid- catch-up growth in weight, and at recovery (normal weight-for-length). VLDL apo B-100 synthesis was determined using stable isotope methodology to measure the rate of incorporation of H-2(3)-leucine into its apoprotein moiety. The fractional and absolute synthesis of VLDL apo B-100 did not differ between the groups or from the initial malnourished stage to the recovery stage. Concentrations of VLDL apo B-100 were greater in the edematous than in the nonedematous group (P < 0.04) and did not differ from the initial stage to recovery. The data indicate that VLDL apo B-100 synthesis is not reduced when children develop either edematous or nonedematous SAM. J. Nutr. 142: 931-935, 2012. C1 [Badaloo, Asha V.; Forrester, Terrence; Reid, Marvin] Univ W Indies, Trop Metab Res Unit, Kingston 7, Jamaica. [Jahoor, Farook] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, Houston, TX 77030 USA. RP Badaloo, AV (reprint author), Univ W Indies, Trop Metab Res Unit, Kingston 7, Jamaica. EM asha.badaloo@uwimona.edu.jm FU NIH [RO1 DK 056689]; USDA/Agricultural Research Service [58-6250-6001] FX Supported in part by NIH grant RO1 DK 056689 and by the USDA/Agricultural Research Service under Cooperative Agreement no. 58-6250-6001. NR 20 TC 1 Z9 1 U1 0 U2 3 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAY PY 2012 VL 142 IS 5 BP 931 EP 935 DI 10.3945/jn.111.155960 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 929TX UT WOS:000303089700018 PM 22437562 ER PT J AU Truong, JT Fu, XY Saltzman, E Al Rajabi, A Dallal, GE Gundberg, CM Booth, SL AF Truong, Jennifer T. Fu, Xueyan Saltzman, Edward Al Rajabi, Ala Dallal, Gerard E. Gundberg, Caren M. Booth, Sarah L. TI Age Group and Sex Do Not Influence Responses of Vitamin K Biomarkers to Changes in Dietary Vitamin K SO JOURNAL OF NUTRITION LA English DT Article ID GAMMA-CARBOXYGLUTAMIC ACID; LIQUID-CHROMATOGRAPHY; OLDER MEN; WOMEN; BONE; SUPPLEMENTATION; OSTEOCALCIN; YOUNGER; PLASMA; URINE AB Inadequate vitamin K intake has been associated with abnormal soft tissue calcification. Older adults may have insufficient intakes of vitamin K and respond less to vitamin K supplementation compared with younger adults. However, little is known about the determinants that influence the response to vitamin K supplementation. Our primary objective was to assess dietary and nondietary determinants of vitamin K status in healthy younger and older adults. In a nonrandomized, nonmasked study, 21 younger (18-40 y) and 21 older (55-80 y) men and women consumed a baseline diet (200 mu g phylloquinone/d) for 5 d, a phylloquinone-restricted diet (10 mu g phylloquinone/d) for 28 d, and a phylloquinone-supplemented diet (500 mu g phylloquinone/d) for 28 d. Changes in vitamin K status markers in response to vitamin K depletion and repletion were studied and the influences of BM I, body fat, and circulating TG were assessed by including them as covariates in the model. Despite baseline differences in measures of vitamin K status, plasma phylloquinone tended to increase (P = 0.07) and the percentage of uncarboxylated osteocalcin and uncarboxylated prothrombin both improved with phylloquinone supplementation (P < 0.007), regardless of age group or sex. Only the excretion of urinary menadione, a vitamin K metabolite, was greater among younger adults in response to depletion than in older adults (P = 0.012), regardless of sex. Adiposity measures and circulating TG did not predict response of any measures. In conclusion, poor vitamin K status can be similarly improved with vitamin K supplementation, regardless of age group or sex. J. Nutr. 142: 936-941, 2012. C1 [Truong, Jennifer T.; Fu, Xueyan; Saltzman, Edward; Al Rajabi, Ala; Dallal, Gerard E.; Booth, Sarah L.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gundberg, Caren M.] Yale Univ, Sch Med, Dept Orthopaed, New Haven, CT USA. RP Booth, SL (reprint author), Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM sarah.booth@tufts.edu OI AL RAJABI, ALA/0000-0003-2426-6279 FU USDA Agricultural Research Service [58-1950-7-707]; NIH [5R01DK69341-4] FX Supported by the USDA Agricultural Research Service under Cooperative Agreement No. 58-1950-7-707 and the NIH (5R01DK69341-4). Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. NR 21 TC 11 Z9 12 U1 0 U2 2 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD MAY PY 2012 VL 142 IS 5 BP 936 EP 941 DI 10.3945/jn.111.154807 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 929TX UT WOS:000303089700019 PM 22437558 ER PT J AU Samaniuk, JR Scott, CT Root, TW Klingenberg, DJ AF Samaniuk, Joseph R. Scott, C. Tim Root, Thatcher W. Klingenberg, Daniel J. TI Rheological modification of corn stover biomass at high solids concentrations SO JOURNAL OF RHEOLOGY LA English DT Article ID APPARENT YIELD-STRESS; FIBER SUSPENSIONS; SLURRIES; KINETICS AB Additives were tested for their ability to modify the rheology of lignocellulosic biomass. Additive types included water-soluble polymers (WSPs), surfactants, and fine particles. WSPs were the most effective rheological modifiers, reducing yield stresses of concentrated biomass by 60-80% for additive concentrations of 1-2 wt. % (based on mass of dry biomass solids). Yield stress and plastic viscosity of rheologically modified biomass depended on WSP molecular weight and degree of substitution. The apparent shear stress-shear rate data are represented with the Bingham model. In the absence of WSP, the biomass exhibited a positive yield stress and a negative plastic viscosity, which suggests a nonmonotonic dependence of shear stress on shear rate. When WSP was added, the yield stress decreased and the plastic viscosity increased, becoming positive for sufficiently large WSP concentrations. (C) 2012 The Society of Rheology. [http://dx.doi.org/10.1122/1.3702101] C1 [Samaniuk, Joseph R.; Root, Thatcher W.; Klingenberg, Daniel J.] Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI 53706 USA. [Samaniuk, Joseph R.; Root, Thatcher W.; Klingenberg, Daniel J.] Univ Wisconsin, Rheol Res Ctr, Madison, WI 53706 USA. [Scott, C. Tim] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Klingenberg, DJ (reprint author), Univ Wisconsin, Dept Chem & Biol Engn, 3006 Engn Hall,1415 Engn Dr, Madison, WI 53706 USA. EM samaniuk@wisc.edu; tscott@fs.fed.us; thatcher@engr.wisc.edu; klingen@engr.wisc.edu RI Samaniuk, Joseph/C-4773-2014 FU U.S. Department of Agriculture [2006-35504-17401, 2010-65504-20406] FX This project was supported by the U.S. Department of Agriculture (NRI Award No. 2006-35504-17401 and AFRI Award No. 2010-65504-20406). NR 31 TC 9 Z9 9 U1 1 U2 36 PU JOURNAL RHEOLOGY AMER INST PHYSICS PI MELVILLE PA STE 1 NO 1, 2 HUNTINGTON QUADRANGLE, MELVILLE, NY 11747-4502 USA SN 0148-6055 J9 J RHEOL JI J. Rheol. PD MAY PY 2012 VL 56 IS 3 BP 649 EP 665 DI 10.1122/1.3702101 PG 17 WC Mechanics SC Mechanics GA 932AM UT WOS:000303261300011 ER PT J AU Cox, WA Thompson, FR Faaborg, J AF Cox, W. Andrew Thompson, Frank R., III Faaborg, John TI Landscape forest cover and edge effects on songbird nest predation vary by nest predator SO LANDSCAPE ECOLOGY LA English DT Article DE Cause-specific mortality; Forest birds; Nest survival; Passerines ID MICE PEROMYSCUS-LEUCOPUS; FRAGMENTED FORESTS; ARTIFICIAL NEST; BIRD COMMUNITY; HABITAT; SUCCESS; SNAKES; METAANALYSIS; HYPOTHESIS; SELECTION AB Rates of nest predation for birds vary between and within species across multiple spatial scales, but we have a poor understanding of which predators drive such patterns. We video-monitored nests and identified predators at 120 nests of the Acadian Flycatcher (Empidonax virescens) and the Indigo Bunting (Passerina cyanea) at eight study sites in Missouri and Illinois, USA, during 2007-2010. We used an information-theoretic approach to evaluate hypotheses concerning factors affecting predator-specific and overall rates of predation at landscape, edge, and nest-site scales. We found support for effects of landscape forest cover and distance to habitat edge. Predation by Brown-headed Cowbirds (Molothrus ater) increased, and predation by rodents decreased as landscape forest cover decreased. Predation by raptors, rodents, and snakes increased as the distance to forest edges decreased, but the effect was modest and conditional upon the top-ranked model. Despite the predator-specific patterns we detected, there was no support for these effects on overall rates of predation. The interactions between breeding birds, nest predators, and the landscapes in which they reside are scale-dependent and context-specific, and may be resistant to broad conceptual management recommendations. C1 [Cox, W. Andrew] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Thompson, Frank R., III] Univ Missouri, USDA, Forest Serv, No Res Stn, Columbia, MO 65211 USA. [Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. RP Cox, WA (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, 302 ABNR, Columbia, MO 65211 USA. EM wac253@mail.missouri.edu OI Cox, William/0000-0002-1205-5979 FU U.S.D.A. Forest Service Northern Research Station; Audubon Society of Missouri; Trans-World Airlines Scholarship program; National Science Foundation; University of Missouri; Faaborg lab FX Funding for this research was provided by the U.S.D.A. Forest Service Northern Research Station, the Audubon Society of Missouri, and the Trans-World Airlines Scholarship program. W.A. Cox was supported by a National Science Foundation Graduate Research Fellowship and a University of Missouri Life Sciences Fellowship when this research was conducted. We thank the many assistants who helped in the field, especially R. Ton, K. Schaeffer, and C. Kukal. We thank the Missouri Department of Conservation for providing housing and access to field sites. W.A. Cox thanks A.S. Cox and the Faaborg lab for advice and support. Finally, we thank the Mark Twain National Forest, Illinois Department of Natural Resources, and the private landowners near Thompsonville, IL for access to their land. NR 62 TC 29 Z9 29 U1 5 U2 90 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD MAY PY 2012 VL 27 IS 5 BP 659 EP 669 DI 10.1007/s10980-012-9711-x PG 11 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA 929JC UT WOS:000303056100004 ER PT J AU Endecott, RL Cox, SH Rubio, CM Loest, CA Hawkins, DE Petersen, MK AF Endecott, R. L. Cox, S. H. Rubio, C. M. Loeest, C. A. Hawkins, D. E. Petersen, M. K. TI Effects of supplements with increasing glucogenic precursor content on reproduction and nutrient utilization in young postpartum range cows SO LIVESTOCK SCIENCE LA English DT Article DE Beef cattle; Glucogenic precursors; Propionate; Protein supplements; Reproduction ID DAIRY-COWS; PROTEIN; ACETATE; GLUCONEOGENESIS; CONCENTRATE; PERFORMANCE; TOLERANCE; RUMINANTS; RESPONSES; GLUCOSE AB Altering nutrient utilization in young postpartum beef cows from milk production to body weight gain has potential to improve reproductive performance. A 2-year study conducted at the Corona Range and Livestock Research Center from February to July in 2003 (n = 33) and 2004 (n = 26) evaluated responses of 2- and 3-year-old postpartum beef cows grazing native range to 2 supplements with increasing glucogenic potential (GP). Supplements were fed at 1135 g.cow(-1). day(-1) twice weekly for approximately 70 days postpartum and provided 1) 341 g CP, 142 g ruminally undegradable protein, 57 g GP (GP57), or 2) 341 g CP, 151 g RUP + 80 g propionate salt (NutroCAL, Kemin Industries, Inc.), 121 g GP (GP121). Supplement x year interactions were observed for days to first estrus (P=0.04) and 24-h milk production at similar to 60 days postpartum (P= 0.04). Cows fed GP57 took longer to return to estrus in 2004 than in 2003, while cows fed GP121 returned to cyclicity in similar days postpartum regardless of year. Cows fed GP57 produced more milk in 2004 than 2003, but cows fed GP121 produced similar amounts of milk regardless of year. Cows had similar (P= 0.61) glucose half-lives after glucose tolerance test at similar to 55 day postpartum (77 and 68 +/- 12 min for GP57 and GP121, respectively). Young cows fed GP121 exhibited consistent milk production and return to estrus regardless of the effects of year. (C) 2012 Elsevier B.V. All rights reserved. C1 [Endecott, R. L.; Cox, S. H.; Rubio, C. M.; Loeest, C. A.; Hawkins, D. E.; Petersen, M. K.] New Mexico State Univ, Dept Anim & Range Sci, Las Cruces, NM 88003 USA. RP Endecott, RL (reprint author), USDA ARS, Ft Keogh LARRL, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM rachel.endecott@montana.edu FU New Mexico Agricultural Experiment Station; F. Valdez of Kemin Industries, Inc FX The authors gratefully acknowledge New Mexico Agricultural Experiment Station and F. Valdez of Kemin Industries, Inc. (Des Moines, IA) for their financial support in this project NR 37 TC 2 Z9 2 U1 0 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 J9 LIVEST SCI JI Livest. Sci. PD MAY PY 2012 VL 145 IS 1-3 BP 109 EP 118 DI 10.1016/j.livsci.2012.01.004 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA 932DK UT WOS:000303270400014 ER PT J AU Anderson, KE Russell, JA Moreau, CS Kautz, S Sullam, KE Hu, Y Basinger, U Mott, BM Buck, N Wheeler, DE AF Anderson, Kirk E. Russell, Jacob A. Moreau, Corrie S. Kautz, Stefanie Sullam, Karen E. Hu, Yi Basinger, Ursula Mott, Brendon M. Buck, Norman Wheeler, Diana E. TI Highly similar microbial communities are shared among related and trophically similar ant species SO MOLECULAR ECOLOGY LA English DT Article DE bacteria; coevolution; Formicidae; herbivores; symbiosis; trophic ecology ID RICE PADDY SOIL; DIGESTIVE-TRACT; BACTERIAL SYMBIONTS; CEPHALOTES-ATRATUS; TETRAPONERA ANTS; OPITUTUS-TERRAE; GUT SYMBIONTS; SP-NOV; APHIDS; FORMICIDAE AB Ants dominate many terrestrial ecosystems, yet we know little about their nutritional physiology and ecology. While traditionally viewed as predators and scavengers, recent isotopic studies revealed that many dominant ant species are functional herbivores. As with other insects with nitrogen-poor diets, it is hypothesized that these ants rely on symbiotic bacteria for nutritional supplementation. In this study, we used cloning and 16S sequencing to further characterize the bacterial flora of several herbivorous ants, while also examining the beta diversity of bacterial communities within and between ant species from different trophic levels. Through estimating phylogenetic overlap between these communities, we tested the hypothesis that ecologically or phylogenetically similar groups of ants harbor similar microbial flora. Our findings reveal: (i) clear differences in bacterial communities harbored by predatory and herbivorous ants; (ii) notable similarities among communities from distantly related herbivorous ants and (iii) similar communities shared by different predatory army ant species. Focusing on one herbivorous ant tribe, the Cephalotini, we detected five major bacterial taxa that likely represent the core microbiota. Metabolic functions of bacterial relatives suggest that these microbes may play roles in fixing, recycling, or upgrading nitrogen. Overall, our findings reveal that similar microbial communities are harbored by ants from similar trophic niches and, to a greater extent, by related ants from the same colonies, species, genera, and tribes. These trends hint at coevolved histories between ants and microbes, suggesting new possibilities for roles of bacteria in the evolution of both herbivores and carnivores from the ant family Formicidae. C1 [Anderson, Kirk E.; Basinger, Ursula; Buck, Norman; Wheeler, Diana E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Anderson, Kirk E.; Mott, Brendon M.] ARS, USDA, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Russell, Jacob A.; Sullam, Karen E.; Hu, Yi] Drexel Univ, Dept Biol, Philadelphia, PA 19104 USA. [Moreau, Corrie S.; Kautz, Stefanie] Field Museum Nat Hist, Dept Zool, Chicago, IL 60605 USA. RP Anderson, KE (reprint author), Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. EM kirk.anderson@ars.usda.gov RI Russell, Jacob/H-7996-2015; OI Moreau, Corrie/0000-0003-1139-5792; Sullam, Karen/0000-0002-1130-2093 FU D.E.W molecular lab (U of A), NSF [1050360]; NSF [1050243]; Grainger Foundation; Drexel University; Pritzker Laboratory for Molecular Systematics and Evolution from the Leopoldina National Academy of Sciences [LPDS 2009-29]; National Institutes of Health [5K-12-GM000708] FX We thank Colin Funaro and Leslie White for technical assistance, and Pedro Agusto da Pos Rodrigues for comments that improved the manuscript. This project was supported in part by the D.E.W molecular lab (U of A), NSF grant 1050360 to J.A.R., NSF grant 1050243 to C.S.M., The Grainger Foundation grant to C.S.M., start-up funding from Drexel University to J.A.R., the Pritzker Laboratory for Molecular Systematics and Evolution, a postdoctoral fellowship (grant LPDS 2009-29) from the Leopoldina National Academy of Sciences to S. K., and an National Institutes of Health-Institutional Research and Academic Career Development Award (5K-12-GM000708) to K.E.A., Students from Drexel University's 2009 Molecular Ecology course also contributed, including Abby Dominy, LeeAnn Haaf, Noga Neeman, Michael Ryan, Haibo Zhang, Samir Patel, Jules Winters, Steve Essinger, John Contreras, Zoe Gautier, and Matt LaMarca. Students from the Field Museum's NSF REU (William Montag and Sara Zufan) and other summer intern programs (Alexandra Gray and Ariadne Lumayag) also contributed to data collection. The USDA/ARS is an equal opportunity employer and provider. NR 79 TC 52 Z9 54 U1 15 U2 111 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0962-1083 J9 MOL ECOL JI Mol. Ecol. PD MAY PY 2012 VL 21 IS 9 BP 2282 EP 2296 DI 10.1111/j.1365-294X.2011.05464.x PG 15 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 927TH UT WOS:000302932500018 PM 22276952 ER PT J AU Valverde, RA Sabanadzovic, S Hammond, J AF Valverde, Rodrigo A. Sabanadzovic, Sead Hammond, John TI Viruses that Enhance the Aesthetics of Some Ornamental Plants: Beauty or Beast? SO PLANT DISEASE LA English DT Article ID WHITEFLY-TRANSMITTED GEMINIVIRUSES; CUCUMBER MOSAIC-VIRUS; YELLOW DWARF DISEASE; TOBACCO-LEAF-CURL; 1ST REPORT; NANDINA-DOMESTICA; FLOWER-BREAKING; RINGSPOT-VIRUS; TRANSMISSION; TOMATO C1 [Valverde, Rodrigo A.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Sabanadzovic, Sead] Mississippi State Univ, Mississippi State, MS 39762 USA. [Hammond, John] ARS, Floral & Nursery Plants Res Unit, USDA, US Natl Arboretum, Beltsville, MD 20705 USA. RP Valverde, RA (reprint author), Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. EM rvalverde@agcenter.lsu.edu OI Sabanadzovic, Sead/0000-0002-2995-2633 NR 99 TC 4 Z9 4 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 600 EP 611 DI 10.1094/PDIS-11-11-0928-FE PG 12 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800001 ER PT J AU Koike, ST Liu, HY Sears, J Tian, TT Daugovish, O Dara, S AF Koike, Steven T. Liu, Hsing-Yeh Sears, John Tian, Tongyan Daugovish, Oleg Dara, Surendra TI Distribution, Cultivar Susceptibility, and Epidemiology of Apium virus Y on Celery in Coastal California SO PLANT DISEASE LA English DT Article ID FAMILY APIACEAE; POISON HEMLOCK; MOSAIC-VIRUS; 1ST REPORT AB Apium virus Y (ApVY) is a potyvirus that was recently found to cause crop loss to celery (Apium gmveolens) in California. Symptoms on leaves exhibit varying forms of chlorosis and necrosis. Depending on the cultivar, celery petioles could also exhibit extensive necrotic, sunken, elongated lesions. Severely affected plants were unmarketable. Disease incidence surveys found that a susceptible celery (cv. 414) showed 55% (2007) and 71% (2008) disease. Because it was noted that the Apiaceae weed poison hemlock (Conium maculation) was present in almost all areas where ApVY affected celery, a 4-year survey collected overwintered hemlock from six coastal county regions and tested composite samples for ApVY using reverse transcription-polymerase chain reaction (RT-PCR) and ApVY-specific primers. These plants were consistently positive for ApVY. Seeds collected from these plants were also positive when tested with the same RTPCR method. However, when ApVY-positive hemlock seeds were germinated and the resulting seedlings tested, all results were negative. The failure of ApVY to be transmitted from hemlock seeds to seedlings was further documented by collecting newly germinated hemlock seedlings from the field and testing them with RT-PCR. All such seedlings were negative for ApVY even though large, adjacent, overwintered hemlock plants tested positive. Two crops of celery seed were produced from ApVY-positive mother plants; celery seed from these infected plants likewise tested positive for ApVY; but seedlings grown from the seed lots were negative for ApVY. Twenty-one celery and celeriac cultivars were inoculated with ApVY using viruliferous aphids. planted in a replicated field trial, and then grown to maturity. Seven cultivars remained symptomless, tested negative for ApVY, and showed signs of possible resistance. The epidemiology of disease caused by ApVY in California evidently involves poison hemlock as a common overwintering host with subsequent vectoring of the virus from hemlock to celery via aphids. ApVY was not seedborne in this weed host or in celery in our experiments. Our data suggest that growers can manage this disease by controlling poison hemlock weed populations and by planting celery cultivars that are not susceptible to ApVY. C1 [Koike, Steven T.] Univ Calif Cooperat Extens, Salinas, CA 93901 USA. [Liu, Hsing-Yeh; Sears, John] ARS, USDA, Crop Improvement & Protect Res Unit, Salinas, CA 93905 USA. [Tian, Tongyan] Calif Dept Food & Agr, Sacramento, CA 95832 USA. [Daugovish, Oleg] Univ Calif Cooperat Extens, Ventura, CA 93003 USA. [Dara, Surendra] Univ Calif Cooperat Extens, San Luis Obispo, CA 93401 USA. RP Koike, ST (reprint author), Univ Calif Cooperat Extens, Salinas, CA 93901 USA. EM stkoike@ucdavis.edu FU California Celery Research Board FX We thank the following for assisting with this study: S. Adams, J. D. Allen, P. Ayala, S. Benzen, A. Breschini, A. Coleman, P. Domingos, W Duncan, R. Hathaway, K. Kammeijer, I. Kite, G. McClellan, J. Politron, R. Pybas, J. Quintero, A. Tilley, and J. Wiley. We especially thank S. Malatesta and L. Pierce for assistance with this work. We acknowledge 3 Star Lettuce, Bejo Seeds, Ferry-Morse Seed Company, Harris Moran Seed Company, and Pybas Vegetable Seed Company for providing seed. We thank Growers Transplanting for providing the celery transplants. We thank Bryce Falk for pre-submission evaluation of the manuscript. This research was supported by the California Celery Research Board. NR 20 TC 1 Z9 1 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 612 EP 617 DI 10.1094/PDIS-08-11-0704 PG 6 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800002 ER PT J AU Mou, B Richardson, K Benzen, S Liu, HY AF Mou, B. Richardson, K. Benzen, S. Liu, H. -Y. TI Effects of Beet necrotic yellow vein virus in Spinach Cultivars SO PLANT DISEASE LA English DT Article ID SUGAR-BEET; BETA-VULGARIS; UNITED-STATES; RESISTANCE; RHIZOMANIA; BNYVV; CALIFORNIA; DISEASE AB Beet necrotic yellow vein virus (BNYVV) causes one of the most economically destructive diseases of sugar beet (Beta vulgaris), rhizomania, which may reduce sugar yield by 80%. This field investigation was conducted to evaluate the interactions between spinach genotypes and different BNYVV strains, and to determine whether BNYVV is transmitted through spinach seeds. Eight commercial spinach cultivars were planted in two BNYVV-infested fields and two control fields in Salinas, CA in 2009. Spinach plants in the BNYVV-infested fields showed disease symptoms of yellow-green or light-green vein clearing, mottling, or yellow-green chlorotic lesions on younger leaves as early as 28 days after planting (four- to six-true leaf stage). Leaves may also become stiff. more crinkled, and necrotic. There was an increase of lateral roots and leaf number but a decrease in leaf weight compared to healthy plants. Infected plants often became stunted, deformed, wilted, and dead. Symptomatic leaves and roots from plants with or without leaf symptoms in BNYVV-infested fields all tested positive for BNYVV by enzyme-linked immunosorbent assay. A more aggressive (resistance-breaking) strain of BNYVV led to higher disease incidence in spinach than in the wild type. BNYVV was not transmitted through spinach seeds. There were significant differences in disease development among cultivars, with disease incidence ranging from 8 to 44%, suggesting that genetic improvement of BNYVV resistance through spinach breeding should be feasible. C1 [Mou, B.; Richardson, K.; Benzen, S.; Liu, H. -Y.] ARS, USDA, Salinas, CA 93905 USA. RP Mou, B (reprint author), ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. EM beiquan.mou@ars.usda.gov FU California Leafy Greens Research Program FX We thank J. Sears, J. Tanaka, R. T. Lewellen, S. Baker, S. Camberos, D. Lara, J. Ochoa, and J. McCreight for technical assistance in the lab and field as well as helpful suggestions, and the California Leafy Greens Research Program for partial funding of the research. NR 29 TC 2 Z9 2 U1 0 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 618 EP 622 DI 10.1094/PDIS-09-11-0748 PG 5 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800003 ER PT J AU Olivera, PD Jin, Y Rouse, M Badebo, A Fetch, T Singh, RP Yahyaoui, A AF Olivera, P. D. Jin, Y. Rouse, M. Badebo, A. Fetch, T., Jr. Singh, R. P. Yahyaoui, A. TI Races of Puccinia graminis f. sp tritici with Combined Virulence to Sr13 and Sr9e in a Field Stem Rust Screening Nursery in Ethiopia SO PLANT DISEASE LA English DT Article ID WHEAT; RESISTANCE; LINES; TTKS; UG99; AFRICA; GENES; SR31; 1RS AB North American durum lines, selected for resistance to TTKSK (Ug99) and related races of Puccinia graminis f. sp. tritici in Kenya, became susceptible in Debre Zeit, Ethiopia, suggesting the presence of stem rust races that were virulent to the TTKSK-effective genes in durum. The objective of this study was to characterize races of P. graminis f. sp. tritici present in the Debre Zeit, Ethiopia stem rust nursery. Three races of P. graminis f. sp. tritici were identified from 34 isolates: JRCQC, TRTTF, and TTKSK. Both races JRCQC and TRTTF possess virulence on stem rust resistance genes Sr13 and Sr9e, which may explain why many TTKSK-resistant durum lines tested in Kenya became susceptible in Debre Zeit. The Sr9e-Sr13 virulence combination is of particular concern because these two genes constitute major components of stem rust resistance in North American durum cultivars. In addition to Sr9e and Sr13 virulence, race TRTTF is virulent to at least three stem rust resistance genes that are effective to race TTKSK, including Sr36, SrTmp, and resistance conferred by the 1AL.1RS rye translocation. Race TRTTF is the first known race with virulence to the stem rust resistance carried by the 1AL.1RS translocation, which represents one of the few effective genes against TTKSK in winter wheat cultivars in the United States. Durum entries exhibiting resistant to moderately susceptible infection response at the Debre Zeit nursery in 2009 were evaluated for reaction to races JRCQC, TRTTF, and TTKSK at the seedling stage. In all, 47 entries were resistant to the three races evaluated at the seedling stage, whereas 26 entries exhibited a susceptible reaction. These results suggest the presence of both major and adult plant resistance genes, which would be useful in durum-wheat-breeding programs. A thorough survey of virulence in the population of P. graminis f. sp. tritici in Ethiopia will allow characterization of the geographic distribution of the races identified in the Debre Zeit field nursery. C1 [Jin, Y.; Rouse, M.] ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Olivera, P. D.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Badebo, A.] Ethiopian Inst Agr Res, Debre Zeit, Ethiopia. [Fetch, T., Jr.] Agr & Agri Food Canada, Cereal Res Ctr, Winnipeg, MB R3T 2M9, Canada. [Singh, R. P.; Yahyaoui, A.] Int Maize & Wheat Improvement Ctr, El Batan 56130, Mexico. RP Jin, Y (reprint author), ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. EM yue.jin@ars.usda.gov RI Rouse, Matthew/G-5474-2011 FU United States Department of Agriculture-Agricultural Research Service; Durable Rust Resistance of Wheat, Cornell FX This research is funded by the United States Department of Agriculture-Agricultural Research Service and the Durable Rust Resistance of Wheat, Cornell. We thank L. Wanschura, S. Gale. C. Kebede. and B. Hibdo for their technical assistance. NR 23 TC 25 Z9 25 U1 0 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 623 EP 628 DI 10.1094/PDIS-09-11-0793 PG 6 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800004 ER PT J AU McClure, MA Nischwitz, C Skantar, AM Schmitt, ME Subbotin, SA AF McClure, Michael A. Nischwitz, Claudia Skantar, Andrea M. Schmitt, Mark E. Subbotin, Sergei A. TI Root-Knot Nematodes in Golf Course Greens of the Western United States SO PLANT DISEASE LA English DT Article ID BENTGRASS PUTTING GREENS; N.-SP NEMATODA; MOLECULAR-IDENTIFICATION; MELOIDOGYNE-CHITWOODI; DNA-SEQUENCES; SUBUNIT RDNA; REGION; EVOLUTION; SPP.; PCR AB A survey of 238 golf courses in 10 states of the western United States found root-knot nematodes (Meloidogyne spp.) in 60% of the putting greens sampled. Sequence and phylogenetic analyses of 18S rRNA, D2-D3 of 28S rRNA, internal transcribed spacer-rRNA, and mitochondrial DNA gene sequences were used to identify specimens from 110 golf courses. The most common species. Meloidogyne naasi, was found in 58 golf courses distributed from Southern California to Washington in the coastal or cooler areas of those states. In the warmer regions of the Southwest, M. marylandi was recovered from 38 golf courses and M. graminis from 11 golf courses. This constitutes the first report of M. marylandi in Arizona, California, Hawaii, Nevada, and Utah, and the first report of M. graminis in Arizona, Hawaii, and Nevada. Two golf courses in Washington were infested with M. minor, the first record of this nematode in the Western Hemisphere. Columbia root-knot nematode, M. chitwoodi, was found in a single golf course in California. Polymerase chain reaction restriction fragment length polymorphism of the intergenic region between the cytochrome oxidase and 16S rRNA genes in the mitochondrial genome with restriction enzyme SspI was able to distinguish populations of M. graminis from M. marylandi, providing a fast and inexpensive method for future diagnosis of these nematodes from turf. C1 [McClure, Michael A.; Schmitt, Mark E.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Nischwitz, Claudia] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Skantar, Andrea M.] ARS, USDA, Nematol Lab, Beltsville, MD 20705 USA. [Subbotin, Sergei A.] Calif Dept Food & Agr, Sacramento, CA 95832 USA. RP McClure, MA (reprint author), Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. EM mcclure@ag.arizona.edu NR 45 TC 24 Z9 24 U1 2 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 635 EP 647 DI 10.1094/PDIS-09-11-0808 PG 13 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800006 ER PT J AU Ochwo-Ssemakula, M Sengooba, T Hakiza, JJ Adipala, E Edema, R Redinbaugh, MG Aritua, V Winter, S AF Ochwo-Ssemakula, M. Sengooba, T. Hakiza, J. J. Adipala, E. Edema, R. Redinbaugh, M. G. Aritua, V. Winter, S. TI Characterization and Distribution of a Potyvirus Associated with Passion Fruit Woodiness Disease in Uganda SO PLANT DISEASE LA English DT Article ID SOUTH-AFRICAN PASSIFLORA; MOSAIC-VIRUS; DETECT MEMBERS; MOTTLE-VIRUS; RNA VIRUS; SEQUENCE; STRAINS; FAMILY; EDULIS; GENUS AB This article describes the incidence and etiology of a viral disease of passion fruit in Uganda. Symptoms, including those characteristic of passion fruit woodiness disease (PWD), were observed on 32% of plants in producing areas. Electron microscopic observations of infected tissues revealed flexuous filaments of ca. 780 nm. Enzyme-linked immunosorbent assays indicated a serological relationship with Cowpea aphid-borne mosaic virus (CABMV) and Passion fruit ringspot virus (PFRSV). In host range studies, only species in the families Solanaceae and Chenopodiaceae were susceptible, and neither Vigna unguiculata nor Phaseolus vulgaris became infected. Coat protein (CP) gene sequences of eight isolates exhibited features typical of potyviruses and were highly similar (88 to 100% identity). However, the sequences had limited sequence identity with CP genes of two of the three potyviruses reported to cause PWD: East Asian Passiflora virus and Passion fruit woodiness virus (PWV). Deduced amino acid sequences for the CP of isolates from Uganda had highest identity with Bean common mosaic necrosis virus (BCMNV) (72 to 79%, with evolutionary divergence values between 0.17 and 0.19) and CABMV (73 to 76%, with divergence values between 0.21 and 0.25). Based on these results and in accordance with International Committee for Taxonomy of Viruses criteria for species demarcation in the family Potyviridae, we conclude that a previously unreported virus causes viral diseases on passion fruit in Uganda. The name "Ugandan Passiflora virus" is proposed for this virus. C1 [Ochwo-Ssemakula, M.] Makerere Univ, Dept Agr Prod, Kampala, Uganda. [Sengooba, T.] Int Food Policy Res Inst, Kampala, Uganda. [Hakiza, J. J.] Natl Agr Res Labs, Kampala, Uganda. [Adipala, E.] Reg Univ Forum Capac Bldg Agr, Kampala, Uganda. [Edema, R.] Makerere Univ, Dept Crop Sci, Kampala, Uganda. [Redinbaugh, M. G.] ARS, USDA, Corn & Soybean Res Unit, Wooster, OH 44691 USA. [Redinbaugh, M. G.] Ohio State Univ, Dept Plant Pathol, OARDC, Wooster, OH 44691 USA. [Aritua, V.] Natl Agr Biotechnol Ctr, Natl Agr Res Labs, Kampala, Uganda. [Aritua, V.] Kansas State Univ, Dept Plant Pathol, Throckmorton Plant Sci Ctr 4024, Manhattan, KS 66506 USA. [Winter, S.] Deutsch Sammlung Mikroorganismen & Zellkulturen G, Plant Virus Dept, D-38104 Braunschweig, Germany. RP Ochwo-Ssemakula, M (reprint author), Makerere Univ, Dept Agr Prod, POB 7062, Kampala, Uganda. EM mknossemakula@agric.mak.ac.ug RI Redinbaugh, Margaret/A-3611-2013 FU Makerere University through the Innovations at Makerere Committee; Rockefeller Foundation [1@Mak/2004/0044]; World Bank [1@Mak/2004/0044]; United States Agency for International Development [EPP-A-00-04-00016-00]; International Foundation for Science [C/3675-1]; Norwegian agency for development cooperation (NORAD); Swedish International Development Agency (Sida-SAREC) FX Funding for this study was provided by Makerere University through the Innovations at Makerere Committee (funded by the Rockefeller Foundation and the World Bank) under Project 1@Mak/2004/0044, the Integrated Pest Management Collaborative Research Support Program (IPM-CRSP) Award No. EPP-A-00-04-00016-00) of the United States Agency for International Development, the International Foundation for Science (Research Grant C/3675-1), and through grants from the Norwegian agency for development cooperation (NORAD) and the Swedish International Development Agency (Sida-SAREC). Technical assistance was provided by the Plant Virology Department at the DSMZ in Braunschweig, Germany, the National Crops Resources Research Institute, the National Agricultural Research Laboratories (NaRL) of NARO, Uganda, and the Biotechnology Laboratory in the Department of Agricultural Production, Makerere University, Uganda. Our gratitude also goes to all the farmers who readily granted permission to inspect their passion fruit fields. NR 39 TC 4 Z9 4 U1 4 U2 13 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 659 EP 665 DI 10.1094/PDIS-03-11-0263 PG 7 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800008 ER PT J AU Pfender, WF Gent, DH Mahaffee, WF AF Pfender, W. F. Gent, D. H. Mahaffee, W. F. TI Sensitivity of Disease Management Decision Aids to Temperature Input Errors Associated with Sampling Interval and Out-of-Canopy Sensor Placement SO PLANT DISEASE LA English DT Article ID RYEGRASS SEED CROPS; POTATO LATE BLIGHT; PERENNIAL RYEGRASS; STEM RUST; MODEL; FORECAST; ROT; SUSCEPTIBILITY; INFORMATION; WASHINGTON AB Many plant disease epidemic models, and the disease management decision aids developed from them, are created based on temperature or other weather conditions measured in or above the crop canopy at intervals of 15 or 30 min. Disease management decision aids, however, commonly are implemented based on hourly weather measurements made from sensors sited at a standard placement of 1.5 m above the ground or are estimated from off-site weather measurements. We investigated temperature measurement errors introduced when sampling interval was increased from 15 to 60 min, and when actual in-canopy conditions were represented by temperature measurements collected by standard-placement sensors (1.5 m above the ground, outside the canopy) in each of three crops (grass seed, grape, and hops) and assessed the impact of these errors on outcomes of decision aids for grass stem rust as well as grape and hops powdery mildews. Decreasing time resolution from 15 to 60 min resulted in statistically significant underestimates of daily maximum temperatures and overestimates of daily minimum temperatures that averaged 0.2 to 0.4 degrees C. Sensor location (in-canopy versus standard-placement) also had a statistically significant effect on measured temperature, and this effect was significantly less in grape or hops than in the grass seed crop. Effects of these temperature errors on performance of disease management decision aids were affected by magnitude of the errors as well as the type of decision aid. The grape and hops powdery mildew decision aids used rule-based indices, and the relatively small (+/- 0.8 degrees C) differences in temperature observed between in-canopy and standard placement sensors in these crops resulted in differences in rule outcomes when actual in-canopy temperatures were near a threshold for declaring that a rule had been met. However, there were only minor differences in the management decision (i.e., fungicide application interval). The decision aid for grass stem rust was a simulation model, for which temperature recording errors associated with location of the weather station resulted in incremental (not threshold) effects on the model of pathogen growth and plant infection probability. Simple algorithms were devised to correct the recorded temperatures or the computed infection probability to produce outcomes similar to those resulting from in-canopy temperature measurements. This study illustrates an example of evaluating (and, if necessary, correcting) temperature measurement errors from weather station sensors not located within the crop canopy, and provides an estimate of uncertainty in temperature measurements associated with location and sampling interval of weather station sensors. C1 [Pfender, W. F.; Gent, D. H.] ARS, USDA, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. [Pfender, W. F.; Gent, D. H.; Mahaffee, W. F.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Mahaffee, W. F.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97331 USA. RP Pfender, WF (reprint author), ARS, USDA, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. EM pfenderw@onid.orst.edu NR 36 TC 1 Z9 1 U1 3 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 726 EP 736 DI 10.1094/PDIS-03-11-0262 PG 11 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800017 ER PT J AU Sthapit, J Gbur, EE Brown-Guedira, G Marshall, DS Milus, EA AF Sthapit, Jinita Gbur, Edward E. Brown-Guedira, Gina Marshall, David S. Milus, Eugene A. TI Characterization of Resistance to Stripe Rust in Contemporary Cultivars and Lines of Winter Wheat from the Eastern United States SO PLANT DISEASE LA English DT Article ID ADULT-PLANT RESISTANCE; F-SP TRITICI; PUCCINIA-STRIIFORMIS; HIGH-TEMPERATURE; GENE LR46; AGGRESSIVENESS; ASSOCIATION; EXPRESSION; EPIDEMICS; MARKERS AB Stripe rust, caused by Puccinia striiformis f. sp. tritici, has been an important disease of winter wheat (Tritium aestivum) in the eastern United States since 2000, when a new strain of the pathogen emerged. The new strain overcame the widely used resistance gene, Yr9, and was more aggressive and better adapted to warmer temperatures than the old strain. Host resistance is the most effective approach to manage stripe rust. Winter wheat lines with resistance to the new strain in the field are common, but the genes conferring this resistance are mostly unknown. The objectives of this research were to characterize the all-stage resistance and adult-plant resistance (APR) to stripe rust in a representative group of contemporary winter wheat cultivars and breeding lines and to identify the resistance genes when possible. Of the 50 lines evaluated for all-stage resistance at the seedling stage, nearly all were susceptible to the new strain. Based on a linked molecular marker, seven lines had resistance gene Yr17 that confers resistance to both old and new strains; however, this resistance was difficult to identify in the seedling stage. Of the 19 lines evaluated for APR, all expressed APR compared with a very susceptible check. Nine had race-specific APR to the new strain and nine had APR to both old and new strains. The remaining line, 26R61, had all-stage resistance to the old strain (conferred by resistance gene Yr9) and a high level of APR to the new strain. APR was expressed as low infection type, low percent leaf area diseased, and long latent period at heading stage under both low and high temperature regimes and could be identified as early as jointing stage. Based on tests for linked molecular markers, the most widely used slow-rusting APR genes, Yr18 and Yr29, were not present in any of the lines. The results of this research indicate that effective all-stage resistance was conferred only by Yr17 and that APR was common and likely conferred by unknown race-specific genes rather than genes conferring slow rusting that are more likely to be durable. C1 [Sthapit, Jinita; Milus, Eugene A.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Gbur, Edward E.] Univ Arkansas, Agr Stat Lab, Fayetteville, AR 72701 USA. [Brown-Guedira, Gina] N Carolina State Univ, USDA, ARS, Plant Sci Res Unit,Dept Crop Sci, Raleigh, NC 27695 USA. [Marshall, David S.] N Carolina State Univ, USDA, ARS, Plant Sci Res Unit,Dept Plant Pathol, Raleigh, NC 27695 USA. RP Milus, EA (reprint author), Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. EM gmilus@uark.edu FU Arkansas Wheat Promotion Board; United States Department of Agriculture-Agricultural Research Service FX We thank D. Moon, J. Hedge, P. Horevaj, and P. Rohman for technical assistance; and the Arkansas Wheat Promotion Board and the United States Department of Agriculture-Agricultural Research Service for financial support. NR 32 TC 5 Z9 6 U1 1 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 737 EP 745 DI 10.1094/PDIS-07-11-0612 PG 9 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800018 ER PT J AU Sikora, EJ Bruton, BD Wayadande, AC Fletcher, J AF Sikora, E. J. Bruton, B. D. Wayadande, A. C. Fletcher, J. TI First Report of the Cucurbit Yellow Vine Disease Caused by Serratia marcescens in Watermelon and Yellow Squash in Alabama. SO PLANT DISEASE LA English DT News Item C1 [Sikora, E. J.] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Bruton, B. D.] ARS, USDA, Lane, OK USA. [Wayadande, A. C.; Fletcher, J.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. RP Sikora, EJ (reprint author), Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. NR 3 TC 4 Z9 5 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 761 EP 761 DI 10.1094/PDIS-09-11-0739-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800026 ER PT J AU Rossman, A Melgar, J Walker, D Gonzales, A Ramirez, T Rivera, J AF Rossman, A. Melgar, J. Walker, D. Gonzales, A. Ramirez, T. Rivera, J. TI First Report of Dolabra nepheliae Associated with Corky Bark Disease of Rambutan and Pulasan in Honduras. SO PLANT DISEASE LA English DT News Item C1 [Rossman, A.; Walker, D.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD USA. [Melgar, J.; Gonzales, A.; Ramirez, T.; Rivera, J.] Honduran Fdn Agr Res, La Lima, Cortes, Honduras. RP Rossman, A (reprint author), ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 765 EP 765 DI 10.1094/PDIS-01-12-0081-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800036 ER PT J AU Nelson, BD Bolton, MD Lopez-Nicora, HD Niblack, TL Mendoza, LD AF Nelson, B. D. Bolton, M. D. Lopez-Nicora, H. D. Niblack, T. L. Mendoza, L. del Rio TI First Confirmed Report of Sugar Beet Cyst Nematode, Heterodera schachtii, in North Dakota. SO PLANT DISEASE LA English DT News Item C1 [Nelson, B. D.; Mendoza, L. del Rio] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Bolton, M. D.] ARS, USDA, Fargo, ND 58102 USA. [Lopez-Nicora, H. D.; Niblack, T. L.] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. RP Nelson, BD (reprint author), N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. NR 4 TC 1 Z9 1 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD MAY PY 2012 VL 96 IS 5 BP 772 EP 772 DI 10.1094/PDIS-02-12-0112-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 929SL UT WOS:000303085800055 ER PT J AU Swoboda, KA Cane, JH AF Swoboda, Katharine A. Cane, James H. TI Breeding biology and incremental benefits of outcrossing for the restoration wildflower, Hedysarum boreale Nutt. (Fabaceae) SO PLANT SPECIES BIOLOGY LA English DT Article DE breeding biology; Fabaceae; fruit set; Hedysarum; outcrossing; seed production ID SWEETVETCH; LEGUMINOSAE AB Northern sweetvetch (Hedysarum boreale Nutt.) is an herbaceous perennial legume of the Rocky Mountains, USA, whose seed is desired for rehabilitating degraded plant communities. Through experimental pollinations, the necessity of pollinators was shown by the failure of autogamy, despite stigmas first becoming receptive in the bud in close proximity to the dehiscing anthers. Nonetheless, the species proved to be self-fertile, initiating as many fruits through selfing as outcrossing. Incremental benefits of outcrossing only later manifested in superior fruit development, seed maturation and seed germination. Farming of H. boreale can yield abundant viable seed if adequately visited by pollinating bees. C1 [Swoboda, Katharine A.; Cane, James H.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Cane, James H.] Utah State Univ, USDA ARS, Pollinating Insects Res Unit, Logan, UT 84322 USA. RP Cane, JH (reprint author), Utah State Univ, Dept Biol, Logan, UT 84322 USA. EM jim.cane@ars.usda.gov FU Great Basin Native Plant Selection; U.S. Department of the Interior, Bureau of Land Management (USDI-BLM) Great Basin Restoration Initiative; U.S. Department of Agriculture, Forest Service (USDA-FS) Rocky Mountain Research Station FX This research was funded by the Great Basin Native Plant Selection and Increase Project funded through the U.S. Department of the Interior, Bureau of Land Management (USDI-BLM) Great Basin Restoration Initiative and the U.S. Department of Agriculture, Forest Service (USDA-FS) Rocky Mountain Research Station. Vince Tepedino and Edward Evans provided detailed, insightful comments on drafts of this manuscript. NR 29 TC 2 Z9 2 U1 2 U2 22 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0913-557X J9 PLANT SPEC BIOL JI Plant Spec. Biol. PD MAY PY 2012 VL 27 IS 2 BP 138 EP 146 DI 10.1111/j.1442-1984.2011.00339.x PG 9 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA 931DO UT WOS:000303195400003 ER PT J AU Gasper, D Barr, B Li, H Taus, N Peterson, R Benjamin, G Hunt, T Pesavento, PA AF Gasper, D. Barr, B. Li, H. Taus, N. Peterson, R. Benjamin, G. Hunt, T. Pesavento, P. A. TI Ibex-Associated Malignant Catarrhal Fever-Like Disease in a Group of Bongo Antelope (Tragelaphus eurycerus) SO VETERINARY PATHOLOGY LA English DT Article DE bongo antelope; histopathology; malignant catarrhal fever; MCFV-ibex; Nubian ibex; polymerase chain reaction; sequencing ID OUTBREAK; FEEDLOT; CATTLE; VIRUS; BISON; PCR AB A private zoological facility experienced an outbreak of malignant catarrhal fever (MCF) in 3 bongo antelope (Tragelaphus eurycerus). All cases were periparturient bongos that presented acutely anorectic beginning similar to 6 weeks after being housed with a Nubian ibex. Disease quickly progressed to respiratory distress and death within 24-72 hours of onset of clinical signs. Consistent gross findings in affected bongos were pulmonary edema and small pale tan foci in the livers. Histological lesions included a nonsuppurative vasculitis in multiple tissues, which is well recognized in MCF, but additionally included necrotizing cholangio-hepatitis and neutrophilic, necrotizing myocarditis. Ibex-associated viral DNA was detected by polymerase chain reaction and was identical in sequence whether derived from bongos or ibex. The sequence closely matched an MCF viral DNA fragment that had been amplified from an ibex and bongo in a previous case report. C1 [Gasper, D.; Pesavento, P. A.] Univ Calif Davis, Sch Vet Med, Dept Pathol, Davis, CA 95616 USA. [Barr, B.] Univ Calif Davis, Sch Vet Med, Calif Anim Hlth & Food Safety Lab, Davis, CA 95616 USA. [Li, H.; Taus, N.] Washington State Univ, Agr Res Serv, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [Peterson, R.] Calif Dept Food & Agr, Anim Hlth Branch, Eureka, CA USA. [Benjamin, G.; Hunt, T.] Safari W Wildlife Preserve, Santa Rosa, CA USA. RP Pesavento, PA (reprint author), Univ Calif Davis, Sch Vet Med, Dept Pathol, Davis, CA 95616 USA. EM papesavento@ucdavis.edu NR 16 TC 3 Z9 3 U1 1 U2 10 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 J9 VET PATHOL JI Vet. Pathol. PD MAY PY 2012 VL 49 IS 3 BP 492 EP 497 DI 10.1177/0300985811429306 PG 6 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA 931QR UT WOS:000303234900009 PM 22194357 ER PT J AU Stewart, LR Bouchard, R Redinbaugh, MG Meulia, T AF Stewart, L. R. Bouchard, R. Redinbaugh, M. G. Meulia, T. TI Complete sequence and development of a full-length infectious clone of an Ohio isolate of Maize dwarf mosaic virus (MDMV) SO VIRUS RESEARCH LA English DT Article DE Maize; Potyvirus; Vascular puncture inoculation; Infectious clone; MDMV ID POTYVIRUS GENOMIC RNA; IN-VITRO TRANSCRIPTS; ZEA-MAYS L.; NUCLEOTIDE-SEQUENCE; VASCULAR PUNCTURE; CDNA-CLONES; SUGARCANE; CHINA; POLYPROTEIN; IDENTIFICATION AB Maize dwarf mosaic virus (MDMV) is an important and widespread aphid-transmitted virus of maize. It is a member of the genus Potyvirus in the family Potyviridae with a monopartite (+) ssRNA genome. Here we report the complete genome sequence and construction and testing of infectious clones of an Ohio isolate of MDMV. Full-length MDMV cDNA was cloned into the vector pSPORT. Full-length cDNA PCR-amplified from the vector constructs were used as template for in vitro transcription, and transcripts were inoculated to maize seeds by vascular puncture inoculation. Plants inoculated by this procedure showed symptoms typical of MDMV infection, and infection was confirmed by RT-PCR and mechanical transmission to new plants. (C) 2012 Elsevier B.V. All rights reserved. C1 [Stewart, L. R.; Redinbaugh, M. G.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Corn & Soybean Res Unit, Wooster, OH 44691 USA. [Bouchard, R.] Ohio State Univ, Hort & Crop Sci Dept, OARDC, Wooster, OH 44691 USA. [Stewart, L. R.; Redinbaugh, M. G.; Meulia, T.] Ohio State Univ, Dept Plant Pathol, OARDC, Wooster, OH 44691 USA. [Meulia, T.] OARDC, MCIC, Wooster, OH 44691 USA. RP Meulia, T (reprint author), OARDC, MCIC, Selby Hall, Wooster, OH 44691 USA. EM meulia.1@osu.edu RI Redinbaugh, Margaret/A-3611-2013 NR 37 TC 10 Z9 11 U1 2 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 J9 VIRUS RES JI Virus Res. PD MAY PY 2012 VL 165 IS 2 BP 219 EP 224 DI 10.1016/j.virusres.2012.02.004 PG 6 WC Virology SC Virology GA 932RK UT WOS:000303308000013 PM 22342423 ER PT J AU O'Shaughnessy, SA Evett, SR Colaizzi, PD Howell, TA AF O'Shaughnessy, Susan A. Evett, Steven R. Colaizzi, Paul D. Howell, Terry A. TI A crop water stress index and time threshold for automatic irrigation scheduling of grain sorghum SO AGRICULTURAL WATER MANAGEMENT LA English DT Article DE Automatic irrigation scheduling; CWSI Time Threshold; Grain sorghum; Plant feedback system ID CANOPY TEMPERATURE; WINTER-WHEAT; SPRAY IRRIGATION; LEPA; EVAPOTRANSPIRATION; SYSTEM; YIELD; CWSI AB Variations of the crop water stress index (CWSI) have been used to characterize plant water stress and schedule irrigations. Usually, this thermal-based stress index has been calculated from measurements taken once daily or over a short period of time, near solar noon or after and in cloud free conditions. A method of integrating the CWSI over a day was developed to avoid the noise that may occur if weather prevents a clear CWSI signal near solar noon. This CWSI and time threshold (CWSI-TT) was the accumulated time that the CWSI was greater than a threshold value (0.45); and it was compared with a time threshold (CWSI-TT) based on a well-watered crop. We investigated the effectiveness of the CWSI-TT to automatically control irrigation of short and long season grain sorghum hybrids (Sorghum bicolor (L.) Moench, NC+ 5C35 and Pioneer 84G62); and to examine crop response to deficit irrigation treatments (i.e. 80%, 55%, 30% and 0% of full replenishment of soil water depletion to 1.5-m depth). Results from automated irrigation scheduling were compared to those from manual irrigation based on weekly neutron probe readings. In 2009, results from the Automatic irrigation were mixed; biomass yields in the 55% and 0% treatments, dry grain yields in the 80% and 0% treatments, and WUE in the 80%, 55%, and 0% treatments were not significantly different from those in the corresponding Manual treatments. However, dry grain yields in the 55% and 30% treatments were significantly less than those in the Manual control plots. These differences were due mainly to soil water variability in the beginning of the growing season. This conclusion is reinforced by the fact that IWUE for dry grain yield was not significantly different for 30% and 55% treatments, and was significantly greater for Automatic control at 80%. In 2010, there were no significant differences in biomass, dry grain yield, WUE, or IWUE for irrigation control methods when compared across the same amount treatments. Similar results between irrigation methods for at least the highest irrigation rate (80% of soil water depletion) in 2009 and among all irrigation treatment amounts in 2010 indicate that the CWSI-TT method can be an effective trigger for automatically scheduling either full or deficit irrigations for grain sorghum in a semi-arid region. Published by Elsevier B.V. C1 [O'Shaughnessy, Susan A.; Evett, Steven R.; Colaizzi, Paul D.; Howell, Terry A.] ARS, USDA, Bushland, TX 79012 USA. RP O'Shaughnessy, SA (reprint author), ARS, USDA, PO Drawer 10,2300 Expt Stn Rd, Bushland, TX 79012 USA. EM Susan.OShaughnessy@ars.usda.gov FU Ogallala Aquifer Program; USDA-Agricultural Research Service; Kansas State University; Texas AgriLife Research; Texas AgriLife Extension Service; Texas Tech University; West Texas AM University; United Sorghum Checkoff Program [R0021-09, R0012-10] FX The authors appreciate the dedicated work performed by Brice Ruthardt, Biological Science Technician, and Luke Britten, Agricultural Science Technician, USDA-ARS, Conservation and Production Research Laboratory, Bushland, TX. Partial funding for this project was from the Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas AgriLife Research, Texas AgriLife Extension Service, Texas Tech University, and West Texas A&M University; and from the United Sorghum Checkoff Program (R0021-09 and R0012-10). NR 34 TC 23 Z9 24 U1 1 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-3774 EI 1873-2283 J9 AGR WATER MANAGE JI Agric. Water Manage. PD MAY PY 2012 VL 107 BP 122 EP 132 DI 10.1016/j.agwat.2012.01.018 PG 11 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 925MO UT WOS:000302765500013 ER PT J AU Friedman, M Levin, CE AF Friedman, Mendel Levin, Carol E. TI Nutritional and medicinal aspects of D-amino acids SO AMINO ACIDS LA English DT Review DE D-Amino acids; D-Peptides; Amino acid derivatives; Nutritional evaluation; Bioavailability; Toxicity ID PERFORMANCE LIQUID-CHROMATOGRAPHY; D-ASPARTATE OXIDASE; SERINE-INDUCED NEPHROTOXICITY; METHIONINE-CONTAINING SOLUTION; NEUROMODULATOR D-SERINE; D-PEPTIDE INHIBITORS; D-THREONINE ALDOLASE; AMYLOID P COMPONENT; BLOOD-BRAIN-BARRIER; WATER RAINBOW-TROUT AB This paper reviews and interprets a method for determining the nutritional value of d-amino acids, d-peptides, and amino acid derivatives using a growth assay in mice fed a synthetic all-amino acid diet. A large number of experiments were carried out in which a molar equivalent of the test compound replaced a nutritionally essential amino acid such as l-lysine (l-Lys), l-methionine (l-Met), l-phenylalanine (l-Phe), and l-tryptophan (l-Trp) as well as the semi-essential amino acids l-cysteine (l-Cys) and l-tyrosine (l-Tyr). The results show wide-ranging variations in the biological utilization of test substances. The method is generally applicable to the determination of the biological utilization and safety of any amino acid derivative as a potential nutritional source of the corresponding l-amino acid. Because the organism is forced to use the d-amino acid or amino acid derivative as the sole source of the essential or semi-essential amino acid being replaced, and because a free amino acid diet allows better control of composition, the use of all-amino-acid diets for such determinations may be preferable to protein-based diets. Also covered are brief summaries of the widely scattered literature on dietary and pharmacological aspects of 27 individual d-amino acids, d-peptides, and isomeric amino acid derivatives and suggested research needs in each of these areas. The described results provide a valuable record and resource for further progress on the multifaceted aspects of d-amino acids in food and biological samples. C1 [Friedman, Mendel; Levin, Carol E.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Friedman, M (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM mendel.friedman@ars.usda.gov OI Levin, Carol/0000-0001-6522-6156; Friedman, Mendel/0000-0003-2582-7517 NR 349 TC 37 Z9 40 U1 10 U2 108 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0939-4451 J9 AMINO ACIDS JI Amino Acids PD MAY PY 2012 VL 42 IS 5 BP 1553 EP 1582 DI 10.1007/s00726-011-0915-1 PG 30 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 926DY UT WOS:000302812700003 PM 21519915 ER PT J AU Fay, JP Richards, GP Ozbay, G AF Fay, Johnna P. Richards, Gary P. Ozbay, Gulnihal TI Water-Quality Parameters and Total Aerobic Bacterial and Vibrionaceae Loads in Eastern Oysters (Crassostrea virginica) From Oyster-Gardening Sites SO ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article ID PHOSPHORUS; DELAWARE; AREAS; BAY AB Oyster gardening is a practice designed to restore habitat for marine life and to improve water quality. This study determined physical and chemical water-quality parameters at two oyster gardening sites in the Delaware Inland Bays and compared them with total aerobic bacteria and Vibrionaceae concentrations in Eastern oysters (Crassostrea virginica). One site was located at the end of a man-made canal, whereas the other was located in an open bay. Measured water parameters included temperature, dissolved oxygen (DO), salinity, pH, total nitrogen, nitrate, nitrite, total phosphorus, and total suspended solids. The highest Vibrionaceae levels, as determined by the colony overlay procedure for peptidases, were at the canal site in September (3.5 x 10(5) g(-1)) and at the bay site in August (1.9 x 10(5) g(-1)). Vibrionaceae levels were significantly greater during the duration of the study at the canal site (P = 0.01). This study provides the first baseline levels for total Vibrionaceae in the Delaware Inland Bays. Minimum DO readings at the bay and canal sites were 3.0 and 2.3 mg l(-1), respectively, far less than the state-targeted minimum threshold of 5.0 mg l(-1). Total phosphorus levels exceeded recommendations of a parts per thousand currency sign0.1 mg l(-1) at the bay and canal sites for all monthly samplings, with mean monthly highs at both sites a parts per thousand yen0.68 mg l(-1) in August. Nitrogen occasionally exceeded the recommended level of 1.0 mg l(-1) at both sites. Overall, waters were highly degraded from high phosphates, nitrogen, and total suspended solids as well as low DO. C1 [Fay, Johnna P.; Ozbay, Gulnihal] Delaware State Univ, Dept Agr & Nat Resources, Dover, DE 19901 USA. [Richards, Gary P.] Delaware State Univ, Agr Res Serv, USDA, Dover, DE 19901 USA. RP Ozbay, G (reprint author), Delaware State Univ, Dept Agr & Nat Resources, Dover, DE 19901 USA. EM gozbay@desu.edu FU National Science Foundation EPSCoR [EPS-0814251]; United States Department of Agriculture (USDA) [DELX0029-06-8] FX We thank the following individuals for their continued support throughout this study: Michael Watson, Gloria Meade, Lori Brown, Jim Kroon, John Ewart, E. J. Chalabala, Wynona Dawson, and Joan Cartwright. Funding for this study was through a National Science Foundation EPSCoR Grant No. EPS-0814251 (G. O.), United States Department of Agriculture (USDA) Evans- Allen Grant No. DELX0029-06-8 (G. O.), and Intramural USDA funding (G. P. R). This study was performed in partial fulfillment of the requirements for a master's degree in Natural Resources in the Agriculture and Natural Resources Graduate Program at Delaware State University. The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. NR 22 TC 0 Z9 0 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0090-4341 J9 ARCH ENVIRON CON TOX JI Arch. Environ. Contam. Toxicol. PD MAY PY 2012 VL 62 IS 4 BP 628 EP 637 DI 10.1007/s00244-011-9736-1 PG 10 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 926CS UT WOS:000302809500008 PM 22183874 ER PT J AU Yiannakouris, N Katsoulis, M Dilis, V Parnell, LD Trichopoulos, D Ordovas, JM Trichopoulou, A AF Yiannakouris, N. Katsoulis, M. Dilis, V. Parnell, L. D. Trichopoulos, D. Ordovas, J. M. Trichopoulou, A. TI Genetic predisposition to coronary heart disease and stroke using an additive genetic risk score: A population-based study in Greece SO ATHEROSCLEROSIS LA English DT Article DE Genetic risk score; Coronary heart disease; Stroke; Myocardial infarction; Cardiovascular disease; Greek-EPIC ID GENOME-WIDE ASSOCIATION; SINGLE NUCLEOTIDE POLYMORPHISMS; ARTERY-DISEASE; MYOCARDIAL-INFARCTION; ISCHEMIC-STROKE; CARDIOVASCULAR-DISEASE; ATRIAL-FIBRILLATION; SEQUENCE VARIANT; 9P21; METAANALYSIS AB Objective: To determine the extent to which the risk for incident coronary heart disease (CHD) increases in relation to a genetic risk score (GRS) that additively integrates the influence of high-risk alleles in nine documented single nucleotide polymorphisms (SNPs) for CHD, and to examine whether this GRS also predicts incident stroke. Methods: Genotypes at nine CHD-relevant SNPs were determined in 494 cases of incident CHD, 320 cases of incident stroke and 1345 unaffected controls drawn from the population-based Greek component of the European Prospective Investigation into Cancer and nutrition (EPIC) cohort. An additive GRS was calculated for each study participant by adding one unit for the presence of each high-risk allele multiplied by the estimated effect size of that allele in the discovery samples. Statistical analysis was performed using logistic regression. Results: The GRS was significantly associated with the incidence of CHD where the odds of CHD incidence in the highest quintile of the GRS were 1.74 times higher (95% confidence interval [CI] = 1.25-2.43, p for trend = 0.0004), compared to the lowest quintile. With respect to stroke, a weaker and non-significant positive association with GRS was apparent as the odds of stroke incidence in the highest quintile of the GRS were 1.36 times higher (95% CI = 0.90-2.06, p for trend = 0.188), compared to the lowest quintile. Conclusion: A GRS relying on nine documented "CHD-specific" SNPs is significantly predictive of CHD but it was not found to be statistically significantly associated with incident stroke. (C) 2012 Elsevier Ireland Ltd. All rights reserved. C1 [Yiannakouris, N.] Harokopio Univ Athens, Div Human Ecol, Athens 17671, Greece. [Katsoulis, M.; Dilis, V.; Trichopoulos, D.; Trichopoulou, A.] Hellen Hlth Fdn, Athens, Greece. [Parnell, L. D.; Ordovas, J. M.] Tufts Univ, HNRCA, Jean Mayer US Dept Agr, Nutr & Genom Lab, Boston, MA 02111 USA. [Trichopoulos, D.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Trichopoulos, D.] Acad Athens, Bur Epidemiol Res, Athens, Greece. [Ordovas, J. M.] CNIC, Dept Cardiovasc Epidemiol & Populat Genet, Madrid, Spain. [Ordovas, J. M.] Inst Madrileno Estudios Avanzados IMDEA Alimentac, Madrid, Spain. [Trichopoulou, A.] Univ Athens, Sch Med, Dept Hyg Epidemiol & Med Stat, WHO Collaborating Ctr Food & Nutr Policies, GR-11527 Athens, Greece. RP Yiannakouris, N (reprint author), Harokopio Univ Athens, Div Human Ecol, 70 El Venizelou St, Athens 17671, Greece. EM nyiannak@hua.gr FU Hellenic Health Foundation; Stavros Niarchros Foundation; National Heart, Lung, and Blood Institute [HL-54776]; National Institute of Diabetes and Digestive and Kidney Diseases [DK075030]; US Department of Agriculture Research [53-K06-5-10, 58-1950-9-001] FX This study was supported by the Hellenic Health Foundation and the Stavros Niarchros Foundation; by National Heart, Lung, and Blood Institute grants HL-54776; National Institute of Diabetes and Digestive and Kidney Diseases, grant number DK075030; and by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research. NR 34 TC 10 Z9 10 U1 0 U2 11 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 J9 ATHEROSCLEROSIS JI Atherosclerosis PD MAY PY 2012 VL 222 IS 1 BP 175 EP 179 DI 10.1016/j.atherosclerosis.2012.02.033 PG 5 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA 928DJ UT WOS:000302960600027 PM 22429504 ER PT J AU Key, N Tallard, G AF Key, Nigel Tallard, Gregoire TI Mitigating methane emissions from livestock: a global analysis of sectoral policies SO CLIMATIC CHANGE LA English DT Article ID GREENHOUSE-GAS MITIGATION; COCONUT OIL; DEVELOPING-COUNTRIES; DAIRY PRODUCTION; ABATEMENT COSTS; CLIMATE-CHANGE; BEEF HEIFERS; AGRICULTURE; SYSTEMS; CATTLE AB Methane emissions from livestock enteric fermentation and manure management represent about 40% of total anthropogenic greenhouse gas emissions from the agriculture sector and are projected to increase substantially in the coming decades, with most of the growth occurring in non-Annex 1 countries. To mitigate livestock methane, incentive policies based on producer-level emissions are generally not feasible because of high administrative costs and producer transaction costs. In contrast, incentive policies based on sectoral emissions are likely administratively feasible, even in developing countries. This study uses an economic model of global agriculture to estimate the effects of two sectoral mitigation policies: a carbon tax and an emissions trading scheme based on average national methane emissions per unit of commodity. The analysis shows how the composition and location of livestock production and emissions change in response to the policies. Results illustrate the importance of global mitigation efforts: when policies are limited to Annex 1 countries, increased methane emissions in non-Annex 1 countries offset approximately two-thirds of Annex 1 emissions reductions. While non-Annex 1 countries face substantial disincentives to enacting domestic carbon taxes, developing countries could benefit from participating in a global sectoral emissions trading scheme. We illustrate one scheme in which non-Annex 1 countries collectively earn USD 2.4 billion annually from methane emission permit sales when methane is priced at USD 30/t CO2-eq. C1 [Key, Nigel] Econ Res Serv, USDA, Washington, DC 20024 USA. [Tallard, Gregoire] Org Econ Cooperat & Dev, F-75775 Paris 16, France. RP Key, N (reprint author), Econ Res Serv, USDA, 355 E St SW, Washington, DC 20024 USA. EM nkey@ers.usda.gov; Gregoire.TALLARD@oecd.org FU Food and Agriculture Organization of the United Nations FX This research was partly supported by the Food and Agriculture Organization of the United Nations. The authors would like to thank Theun Vellinga, Pierre Gerber, Carolyn Opio, Henning Steinfeld, and Merritt Cluff for helpful insights and assistance. We would also like to thank three anonymous reviewers for their valuable comments. The views expressed are the authors' and do not necessarily reflect those of the Economic Research Service, USDA or the OECD. NR 58 TC 13 Z9 13 U1 5 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 J9 CLIMATIC CHANGE JI Clim. Change PD MAY PY 2012 VL 112 IS 2 BP 387 EP 414 DI 10.1007/s10584-011-0206-6 PG 28 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 919LJ UT WOS:000302327700012 ER PT J AU Hung, HY Browne, C Guill, K Coles, N Eller, M Garcia, A Lepak, N Melia-Hancock, S Oropeza-Rosas, M Salvo, S Upadyayula, N Buckler, ES Flint-Garcia, S McMullen, MD Rocheford, TR Holland, JB AF Hung, H-Y Browne, C. Guill, K. Coles, N. Eller, M. Garcia, A. Lepak, N. Melia-Hancock, S. Oropeza-Rosas, M. Salvo, S. Upadyayula, N. Buckler, E. S. Flint-Garcia, S. McMullen, M. D. Rocheford, T. R. Holland, J. B. TI The relationship between parental genetic or phenotypic divergence and progeny variation in the maize nested association mapping population SO HEREDITY LA English DT Article DE genetic variance; heritability; QTL mapping ID GENOME-WIDE ASSOCIATION; GENEALOGICAL DISTANCE; SPATIAL-ANALYSIS; VARIANCE; SELECTION; WHEAT; ARCHITECTURE; PREDICTION; DIVERSITY; HETEROSIS AB Appropriate selection of parents for the development of mapping populations is pivotal to maximizing the power of quantitative trait loci detection. Trait genotypic variation within a family is indicative of the family's informativeness for genetic studies. Accurate prediction of the most useful parental combinations within a species would help guide quantitative genetics studies. We tested the reliability of genotypic and phenotypic distance estimators between pairs of maize inbred lines to predict genotypic variation for quantitative traits within families derived from biparental crosses. We developed 25 families composed of similar to 200 random recombinant inbred lines each from crosses between a common reference parent inbred, B73, and 25 diverse maize inbreds. Parents and families were evaluated for 19 quantitative traits across up to 11 environments. Genetic distances (GDs) among parents were estimated with 44 simple sequence repeat and 2303 single-nucleotide polymorphism markers. GDs among parents had no predictive value for progeny variation, which is most likely due to the choice of neutral markers. In contrast, we observed for about half of the traits measured a positive correlation between phenotypic parental distances and within-family genetic variance estimates. Consequently, the choice of promising segregating populations can be based on selecting phenotypically diverse parents. These results are congruent with models of genetic architecture that posit numerous genes affecting quantitative traits, each segregating for allelic series, with dispersal of allelic effects across diverse genetic material. This architecture, common to many quantitative traits in maize, limits the predictive value of parental genotypic or phenotypic values on progeny variance. Heredity (2012) 108, 490-499; doi:10.1038/hdy.2011.103; published online 26 October 2011 C1 [Browne, C.; Guill, K.; Garcia, A.; Lepak, N.; Melia-Hancock, S.; Buckler, E. S.; Flint-Garcia, S.; McMullen, M. D.; Holland, J. B.] N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. [Hung, H-Y; Coles, N.; Eller, M.; Oropeza-Rosas, M.; Salvo, S.; Holland, J. B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Upadyayula, N.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Buckler, E. S.] Cornell Univ, Inst Genom Divers, Ithaca, NY USA. [Buckler, E. S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY USA. [Flint-Garcia, S.; McMullen, M. D.] Univ Missouri, Div Plant Sci, Columbia, MO USA. [Rocheford, T. R.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Holland, JB (reprint author), N Carolina State Univ, USDA ARS, Raleigh, NC 27695 USA. EM james_holland@ncsu.edu OI Buckler, Edward/0000-0002-3100-371X; Holland, James/0000-0002-4341-9675 FU National Science Foundation [DBI-0321467, IOS-0820619, IOS-0604923]; USDA-ARS; NC State University FX This research was supported by the National Science Foundation (DBI-0321467, IOS-0820619 and IOS-0604923) and funds provided by USDA-ARS (to ESB, JBH and MDM) and NC State University (to HH). We thank all staffs of the maize breeding programs at the NC State University and Central Crops Research Station. We also thank the editor and anonymous reviewers, particularly for drawing our attention to sampling to maximize total genetic variation. NR 54 TC 30 Z9 30 U1 1 U2 38 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X J9 HEREDITY JI Heredity PD MAY PY 2012 VL 108 IS 5 BP 490 EP 499 DI 10.1038/hdy.2011.103 PG 10 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 928RS UT WOS:000303001600006 PM 22027895 ER PT J AU Hachana, Y Paape, MJ AF Hachana, Y. Paape, Max J. TI Physical and chemical characteristics of yoghurt produced from whole milk with different levels of somatic cell counts SO INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION LA English DT Article DE apparent viscosity; proteolysis; lipolysis; somatic cell count; yoghurt quality ID PASTEURIZED FLUID MILK; BOVINE-MILK; SHELF-LIFE; UHT MILK; MASTITIS; PROTEOLYSIS; LIPOLYSIS; NITROGEN; STORAGE; CHEESE AB For yoghurts were made from milk with different levels of somatic cell count (SCC) (low 95,000 cells ml(-1), intermediate 398,000 cells ml(-1) and high 1,150,000 cells ml(-1)). Yoghurt samples were analysed for the degree of proteolysis, lipolysis (free fatty acid (FFA) content), acidity, pH and apparent viscosity on days 1, 14 and 28. The SCC had no significant effect (p > 0.05) on either the acidity or the pH of the yoghurt after 1 day of cold storage. However, significant effects (p < 0.05) of SCC were observed after 14 and 28 days of storage. Yoghurt samples made from intermediate and high SCC milk showed higher viscosity (p < 0.05) and lower (p < 0.05) casein content on days 14 and 28 of cold storage than yoghurt made from low SCC milk. High FFA concentrations (p < 0.05) were observed only in yoghurt made from high SCC milk. High SCC in milk increased both proteolysis and lipolysis in yoghurt during storage. C1 [Hachana, Y.] Super Inst Agr, Dept Anim Sci, Chott Meriem 4042, Sousse, Tunisia. [Paape, Max J.] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD 20705 USA. RP Hachana, Y (reprint author), Rue Olivier Khezama Est 4051, Sousse, Tunisia. EM hachana@yahoo.fr NR 45 TC 1 Z9 1 U1 3 U2 21 PU INFORMA HEALTHCARE PI LONDON PA TELEPHONE HOUSE, 69-77 PAUL STREET, LONDON EC2A 4LQ, ENGLAND SN 0963-7486 J9 INT J FOOD SCI NUTR JI Int. J. Food Sci. Nutr. PD MAY PY 2012 VL 63 IS 3 BP 303 EP 309 DI 10.3109/09637486.2011.627839 PG 7 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA 926CJ UT WOS:000302808600009 PM 21999630 ER PT J AU Stark, LR Brinda, JC McLetchie, DN Oliver, MJ AF Stark, Lloyd R. Brinda, John C. McLetchie, D. Nicholas Oliver, Melvin J. TI EXTENDED PERIODS OF HYDRATION DO NOT ELICIT DEHARDENING TO DESICCATION TOLERANCE IN REGENERATION TRIALS OF THE MOSS SYNTRICHIA CANINERVIS SO INTERNATIONAL JOURNAL OF PLANT SCIENCES LA English DT Article DE hardening; deacclimation; CO2 enrichment; chlorosis; constitutive desiccation tolerance; rapid-dry ID INCREASED NITROGEN DEPOSITION; ELEVATED ATMOSPHERIC CO2; BIOLOGICAL SOIL CRUSTS; ATRICHUM-ANDROGYNUM; TORTULA-RURALIS; POLYTRICHUM-FORMOSUM; ABSCISIC-ACID; PHOTOSYNTHETIC RESPONSES; PHYSCOMITRELLA-PATENS; PHYSIOLOGICAL-ASPECTS AB Dehardening (deacclimation) to water stress is an integral phase of desiccation tolerance (DT) in bryophytes. Shoots of the desert moss Syntrichia caninervis were harvested and dehardened after exposure to field CO2 concentrations of 380 ppm (ambient) and 550 ppm (elevated). The dehardening period consists of three phases, with shoots from the first phase (3-7 d) the least able to recover from a rapid-drying event and the second (12-18 d) and third (21-27 d) phases characterized by shoots hardening to desiccation and approaching control levels. Response variables following this pattern include tissue chlorosis, protonemal emergence and area, shoot growth resumption, total regenerative shoot production, and probability of fungal attack. Dehydrin levels did not increase over time during the 27-d experimental hydrating period and thus could not account for the observed constitutive hardening to DT. This first experimental sequence on extended dehardening in bryophytes counters the assumption that field shoots hydrated longer than four consecutive days are entirely dehardened and provides evidence that mosses can harden to DT without relying on external cues such as partial hydration. We propose that the "dehardening phase'' is a recovery phase during which plants are somewhat dehardened, and, following recovery, the plants enter a constitutive protective phase. C1 [Stark, Lloyd R.; Brinda, John C.] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA. [McLetchie, D. Nicholas] Univ Kentucky, Dept Biol, Lexington, KY 40506 USA. [Oliver, Melvin J.] Univ Missouri, USDA, ARS, MWA,PGRU, Columbia, MO 65211 USA. RP Stark, LR (reprint author), Univ Nevada, Sch Life Sci, 4505 Maryland Pkwy, Las Vegas, NV 89154 USA. EM lrs@unlv.nevada.edu OI Brinda, John/0000-0001-9083-1235 FU U.S. Department of Energy (DOE), through the Western Regional Center of the National Institute for Climate Change Research [DE-FCO2-03ER63613]; NSF [IOB 0416407]; UNLV faculty FX We thank Lynn Fenstermaker, Dene Charlet, and Jon Titus for installation of the initial field site treatments; Eric Knight and Derek Babcock for providing field assistance; Jim Elder for expert technical help; and Stan Smith for site operations. Crystal Erickson prepared media and solutions for the experiment. This research was supported by the Office of Science, Biological and Environmental Research Program, U.S. Department of Energy (DOE), through the Western Regional Center of the National Institute for Climate Change Research (formerly known as the National Institute for Global Environmental Change) under cooperative agreement DE-FCO2-03ER63613. Financial support does not constitute an endorsement by the DOE of the views expressed in this article. The mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA or DOE and does not imply its approval to the exclusion of other products that may also be suitable. D. N. McLetchie was supported by NSF grant IOB 0416407. L. R. Stark was supported by a UNLV faculty sabbatical leave during a portion of this project. NR 87 TC 8 Z9 8 U1 0 U2 19 PU UNIV CHICAGO PRESS PI CHICAGO PA 1427 E 60TH ST, CHICAGO, IL 60637-2954 USA SN 1058-5893 J9 INT J PLANT SCI JI Int. J. Plant Sci. PD MAY PY 2012 VL 173 IS 4 BP 333 EP 343 DI 10.1086/663970 PG 11 WC Plant Sciences SC Plant Sciences GA 928EC UT WOS:000302963000001 ER PT J AU Williams, LE Baeza, P Vaughn, P AF Williams, L. E. Baeza, P. Vaughn, P. TI Midday measurements of leaf water potential and stomatal conductance are highly correlated with daily water use of Thompson Seedless grapevines SO IRRIGATION SCIENCE LA English DT Article ID VITIS-VINIFERA L.; GIBBERELLIC-ACID APPLICATION; NET CO2 ASSIMILATION; DEFICIT IRRIGATION; SAP FLOW; SENSITIVE INDICATOR; FIELD CONDITIONS; USE EFFICIENCY; TRANSPIRATION; PHOTOSYNTHESIS AB A study was conducted to determine the relationship between midday measurements of vine water status and daily water use of grapevines measured with a weighing lysimeter. Water applications to the vines were terminated on August 24th for 9 days and again on September 14th for 22 days. Daily water use of the vines in the lysimeter (ETLYS) was approximately 40 L vine(-1) (5.3 mm) prior to turning the pump off, and it decreased to 22.3 L vine(-1) by September 2nd. Pre-dawn leaf water potential (I-PD) and midday I-l on August 24th were -0.075 and -0.76 MPa, respectively, with midday I-l decreasing to -1.28 MPa on September 2nd. Leaf g (s) decreased from similar to 500 to similar to 200 mmol m(-2) s(-1) during the two dry-down periods. Midday measurements of g (s) and I-l were significantly correlated with one another (r = 0.96) and both with ETLYS/ETo (r = similar to 0.9). The decreases in I-l, g (s), and ETLYS/ETo in this study were also a linear function of the decrease in volumetric soil water content. The results indicate that even modest water stress can greatly reduce grapevine water use and that short-term measures of vine water status taken at midday are a reflection of daily grapevine water use. C1 [Williams, L. E.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Williams, L. E.] Kearney Agr Ctr, Parlier, CA 93648 USA. [Baeza, P.] Univ Politecn Madrid, Dept Prod Vegetal Fitotecnia, E-28040 Madrid, Spain. [Vaughn, P.] ARS, Water Management Res Lab, USDA, Parlier, CA 93648 USA. RP Williams, LE (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM williams@uckac.edu FU American Vineyard Foundation; Viticulture Consortium; California Competitive Grants for Viticulture and Enology; Programa Nacional para la Movilidad de Profesores de Universidad del Ministerio de Educacion y Ciencia de Espana FX This study was funded in part by grants from the American Vineyard Foundation, Viticulture Consortium and the California Competitive Grants for Viticulture and Enology. Dr. Baeza was supported by a grant from the Programa Nacional para la Movilidad de Profesores de Universidad del Ministerio de Educacion y Ciencia de Espana. NR 66 TC 25 Z9 26 U1 1 U2 38 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD MAY PY 2012 VL 30 IS 3 BP 201 EP 212 DI 10.1007/s00271-011-0276-2 PG 12 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 925HT UT WOS:000302752800004 ER PT J AU Oakley, BB Carbonero, F Dowd, SE Hawkins, RJ Purdy, KJ AF Oakley, Brian B. Carbonero, Franck Dowd, Scot E. Hawkins, Robert J. Purdy, Kevin J. TI Contrasting patterns of niche partitioning between two anaerobic terminal oxidizers of organic matter SO ISME JOURNAL LA English DT Article DE methanogenesis; niche partitioning; pyrosequencing; sulfate reduction ID TARGETED OLIGONUCLEOTIDE PROBES; SULFATE-REDUCING BACTERIA; RIBOSOMAL-RNA; MICROBIAL COMMUNITIES; RARE BIOSPHERE; SP NOV.; DIVERSITY; SEDIMENTS; METHANOSAETA; ENVIRONMENT AB Understanding the ecological principles underlying the structure and function of microbial communities remains an important goal for microbial ecology. We examined two biogeochemically important taxa, the sulfate-reducing bacterial genus, Desulfobulbus, and the methanogenic archaeal genus, Methanosaeta, to compare and contrast niche partitioning by these two taxa that are ecologically linked as anaerobic terminal oxidizers of organic material. An observational approach utilizing functional gene pyrosequencing was combined with a community-based reciprocal incubation experiment and characterization of a novel Desulfobulbus isolate. To analyze the pyrosequencing data, we constructed a data analysis pipeline, which we validated with several control data sets. For both taxa, particular genotypes were clearly associated with certain portions of an estuarine gradient, consistent with habitat or niche partitioning. Methanosaeta genotypes were generally divided between those found almost exclusively in the marine habitat (similar to 30% of operational taxonomic units (OTUs)), and those which were ubiquitously distributed across all or most of the estuary (similar to 70% of OTUs). In contrast to this relatively monotonic distribution, for Desulfobulbus, there were many more genotypes, and their distributions represented a wide range of inferred niche widths from specialist genotypes found only at a single site, to ubiquitous or generalist genotypes found in all 10 sites examined along the full estuarine gradient. Incubation experiments clearly showed that, for both taxa, communities from opposite ends of the estuary did not come to resemble one another, regardless of the chemical environment. Growth of a Desulfobulbus isolated into pure culture indicated that the potential niche of this organism is significantly larger than the realized niche. We concluded that niche partitioning can be an important force structuring microbial populations, with biotic and abiotic components having very different effects depending on the physiology and ecology of each taxon. The ISME Journal (2012) 6, 905-914; doi:10.1038/ismej.2011.165; published online 24 November 2011 C1 [Oakley, Brian B.; Carbonero, Franck; Purdy, Kevin J.] Univ Warwick, Sch Life Sci, Coventry CV4 7AL, W Midlands, England. [Dowd, Scot E.] Res & Testing Labs, Lubbock, TX USA. [Hawkins, Robert J.] Univ Reading, Dept Biol Sci, Reading, Berks, England. RP Oakley, BB (reprint author), Agr Res Serv, USDA, Richard Russell Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM brian.oakley@ars.usda.gov RI Purdy, Kevin/A-1900-2009 OI Purdy, Kevin/0000-0002-2997-9342 FU Marie Curie Excellence [MEXT-CT-2005-024112]; UK Natural Environment Research Council FX This study is part of the Marie Curie Excellence Grant for Teams project, MicroComXT, (MEXT-CT-2005-024112) funded under FP6. RJH was supported by the UK Natural Environment Research Council. We thank Tom Lie, Martin Ostrowski, Andy Millard and Kevin Gaston for helpful discussions. NR 58 TC 13 Z9 13 U1 1 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 J9 ISME J JI ISME J. PD MAY PY 2012 VL 6 IS 5 BP 905 EP 914 DI 10.1038/ismej.2011.165 PG 10 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA 928AA UT WOS:000302950700001 PM 22113373 ER PT J AU Farina, EK Kiel, DP Roubenoff, R Schaefer, EJ Cupples, LA Tucker, KL AF Farina, Emily K. Kiel, Douglas P. Roubenoff, Ronenn Schaefer, Ernst J. Cupples, L. Adrienne Tucker, Katherine L. TI Plasma phosphatidylcholine concentrations of polyunsaturated fatty acids are differentially associated with hip bone mineral density and hip fracture in older adults: The framingham osteoporosis study SO JOURNAL OF BONE AND MINERAL RESEARCH LA English DT Article DE DOCOSAHEXAENOIC ACID; LINOLEIC ACID; ARACHIDONIC ACID; BMD; FRACTURE ID NF-KAPPA-B; IDIOPATHIC CALCIUM NEPHROLITHIASIS; DOCOSAHEXAENOIC ACID; EICOSAPENTAENOIC ACID; ARACHIDONIC-ACID; PPAR-GAMMA; LIPID-PEROXIDATION; ENDOTHELIAL-CELLS; HUMAN OSTEOBLASTS; LINOLENIC ACID AB Polyunsaturated fatty acids (PUFAs) may influence bone health. The objective of this work was to examine associations between plasma phosphatidylcholine (PC) PUFA concentrations and hip measures: (1) femoral neck bone mineral density (FN-BMD) (n?=?765); (2) 4-year change in FN-BMD (n?=?556); and (3) hip fracture risk (n?=?765) over 17-year follow-up among older adults in the Framingham Osteoporosis Study. BMD measures were regressed on quintile of plasma PC PUFAs (docosahexaenoic acid [DHA], linoleic acid [LA], and arachidonic acid [AA]), adjusted for covariates. Hazard ratios (HR) and 95% confidence interval (CI) for hip fracture were estimated by quintile of plasma PC PUFAs, adjusted for covariates. Higher concentrations of PC DHA were associated with loss of FN-BMD over 4 years in women (p-trend?=?0.04), but was protective in men in the uppermost quintile compared to men grouped in the lower four quintiles, in post hoc analysis (p?=?0.01). PC LA concentrations were inversely associated with baseline FN-BMD in women (p-trend?=?0.02), and increased hip fracture risk in women and men (p-trend?=?0.05), but body mass index (BMI) adjustment attenuated these associations (p-trend?=?0.12 and p-trend?=?0.14, respectively). A trend toward a protective association was observed between PC AA and baseline FN-BMD in men (p-trend?=?0.06). Women and men with the highest PC AA concentrations had 51% lower hip fracture risk than those with the lowest (HR?=?0.49, 95% CI?=?0.241.00). Opposing effects of PC DHA on FN-BMD loss observed in women and men need further clarification. Bone loss associated with PC LA may be confounded by BMI. High PC AA concentrations may be associated with reduced hip fracture risk. (c) 2012 American Society for Bone and Mineral Research. C1 [Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Bouve Coll Hlth Sci, Boston, MA 02115 USA. [Farina, Emily K.; Schaefer, Ernst J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Kiel, Douglas P.] Harvard Univ, Sch Med, Boston, MA USA. [Kiel, Douglas P.] Hebrew SeniorLife, Inst Aging Res, Boston, MA USA. [Roubenoff, Ronenn] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Boston, MA USA. RP Tucker, KL (reprint author), Northeastern Univ, Dept Hlth Sci, Bouve Coll Hlth Sci, 316 Robinson Hall, Boston, MA 02115 USA. EM kl.tucker@neu.edu OI Cupples, L. Adrienne/0000-0003-0273-7965; Kiel, Douglas/0000-0001-8474-0310; Tucker, Katherine/0000-0001-7640-662X FU National Institute of Arthritis Musculoskeletal and Skin Diseases; National Institute on Aging [R01 AR/AG 41398]; National Heart, Lung, and Blood Institute (NHLBI) Framingham Heart Study (National Institutes of Health/NHLBI), Framingham, MA, USA [N01-HC-25195] FX This study was supported by a grant from the National Institute of Arthritis Musculoskeletal and Skin Diseases and the National Institute on Aging R01 AR/AG 41398 and the National Heart, Lung, and Blood Institute (NHLBI) Framingham Heart Study (National Institutes of Health/NHLBI contract N01-HC-25195, Bethesda, MD), Framingham, MA, USA. NR 67 TC 11 Z9 11 U1 0 U2 7 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0884-0431 J9 J BONE MINER RES JI J. Bone Miner. Res. PD MAY PY 2012 VL 27 IS 5 BP 1222 EP 1230 DI 10.1002/jbmr.1581 PG 9 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 927VW UT WOS:000302939400026 PM 22392875 ER PT J AU Das, A Babiuk, S McIntosh, MT AF Das, Amaresh Babiuk, Shawn McIntosh, Michael T. TI Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID HYDROXYNAPHTHOL BLUE-DYE; LUMPY SKIN-DISEASE; VISUAL DETECTION; INFECTIOUS-DISEASES; CLINICAL-DIAGNOSIS; ESCHERICHIA-COLI; VIRUS; SHEEP; LAMP; GENE AB Sheep pox (SP), goat pox (GP), and lumpy skin disease (LSD), caused by capripoxviruses (CaPVs), are economically important diseases of sheep, goats, and cattle, respectively. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of CaPVs. LAMP primers were designed to target a conserved gene encoding the poly(A) polymerase small subunit (VP39) of CaPVs. Hydroxynaphthol blue (HNB) was incorporated to monitor assay progress by color change from violet when negative to sky blue when positive, and results were verified by agarose gel electrophoresis. The LAMP assay was shown to be highly specific for CaPVs, with no apparent cross-reactivity to other related viruses (near neighbors) or viruses that cause similar clinical signs (look-a-like viruses). The performance of LAMP was compared to that of a highly sensitive quantitative real-time PCR (qPCR) assay. LAMP and qPCR exhibited similar analytical sensitivities, with limits of detection of 3 and 8 viral genome copies, respectively. Diagnostic specificity was assessed on 36 negative specimens, including swabs and EDTA blood from control sheep, goats, and cattle. Diagnostic sensitivity was assessed on 275 specimens, including EDTA blood, swabs, and tissues from experimentally infected sheep, goats, and cattle. Overall agreement on diagnostic test results between the two assays was 90 to 95% for specificity and 89 to 100% for sensitivity. The LAMP assay described in this report is simple to use, inexpensive, highly sensitive, and particularly well suited for the diagnosis of capripox in less well equipped laboratories and in rural settings where resources are limited. C1 [Das, Amaresh; McIntosh, Michael T.] Anim & Plant Hlth Inspect Serv, Foreign Anim Dis Diagnost Lab, Natl Vet Serv Labs, USDA,Plum Isl Anim Dis Ctr, Orient Point, NY USA. [Babiuk, Shawn] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada. RP Das, A (reprint author), Anim & Plant Hlth Inspect Serv, Foreign Anim Dis Diagnost Lab, Natl Vet Serv Labs, USDA,Plum Isl Anim Dis Ctr, Orient Point, NY USA. EM amaresh.das@aphis.usda.gov FU Australian Woolgrowers; Australian Government [EC145]; U.S. Department of Agriculture (USDA), Animal and Plant Health Inspection Service, National Veterinary Services Laboratories; USDA Agricultural Research Service FX Financial support for generating the samples was provided by the Australian Woolgrowers and the Australian Government through Australian Wool Innovation Limited Project no. EC145. We thank Michael Pushka from the Canadian Food Inspection Agency, National Centre for Foreign Animal Disease, Winnipeg, Manitoba, Canada, for providing support for shipment of the experimental samples used in this study. This study was supported by the U.S. Department of Agriculture (USDA), Animal and Plant Health Inspection Service, National Veterinary Services Laboratories, and by interagency agreement with the USDA Agricultural Research Service. NR 36 TC 17 Z9 21 U1 0 U2 20 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD MAY PY 2012 VL 50 IS 5 BP 1613 EP 1620 DI 10.1128/JCM.06796-11 PG 8 WC Microbiology SC Microbiology GA 927II UT WOS:000302900500018 PM 22357504 ER PT J AU Donahue, M Godden, SM Bey, R Wells, S Oakes, JM Sreevatsan, S Stabel, J Fetrow, J AF Donahue, M. Godden, S. M. Bey, R. Wells, S. Oakes, J. M. Sreevatsan, S. Stabel, J. Fetrow, J. TI Heat treatment of colostrum on commercial dairy farms decreases colostrum microbial counts while maintaining colostrum immunoglobulin G concentrations SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE colostrum; immunoglobulin; bacteria; heat treatment ID BOVINE COLOSTRUM; PASSIVE-IMMUNITY; MYCOBACTERIUM-PARATUBERCULOSIS; TREATED COLOSTRUM; CALVES; HEIFERS; MILK; COWS; PASTEURIZATION; DENATURATION AB This study was conducted on 6 commercial dairy farms in Minnesota and Wisconsin to describe the effect of heat treatment (at 60 degrees C for 60 min) on colostrum, on colostrum bacteria counts, and immunoglobulin G concentrations. First-milking colostrum was collected each day, pooled, divided into 2 aliquots, and then 1 aliquot was heat treated in a commercial batch pasteurizer at 60 degrees C for 60 min. Frozen samples of pre- and post- heat-treated colostrum were submitted for standard microbial culture (total plate count and total coliform count, cfu/mL) and testing for immunoglobulin G concentrations (mg/mL). Data were analyzed from 266 unique batches of colostrum. Linear regression showed that heat treatment decreased colostrum total plate counts (-2.25 log(10)) and coliform counts (-2.49 log(10)), but, overall, did not affect colostrum IgG concentration. Though higher-quality batches of colostrum did experience a greater magnitude of loss of IgG as a result of heat treatment as compared with lower- or intermediate-quality batches of colostrum, the colostral IgG concentrations in these batches remained high overall, and within acceptable limits for feeding. This study demonstrates that batch heat treatment of colostrum at 60 C for 60 min can be successfully conducted on commercial dairy farms by farm staff to decrease colostrum microbial counts while maintaining colostrum IgG concentrations. C1 [Donahue, M.; Godden, S. M.; Bey, R.; Wells, S.; Sreevatsan, S.; Fetrow, J.] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. [Oakes, J. M.] Univ Minnesota, Sch Publ Hlth, Div Epidemiol & Community Hlth, Minneapolis, MN 55454 USA. [Stabel, J.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Godden, SM (reprint author), Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, St Paul, MN 55108 USA. EM godde002@umn.edu OI Sreevatsan, Srinand/0000-0002-5162-2403 FU US Department of Agriculture-Cooperative State Research, Education, and Extension Service (USDA-CSREES) FX This study was funded by a grant from the US Department of Agriculture-Cooperative State Research, Education, and Extension Service (USDA-CSREES). The authors thank the owners and managers of the 6 dairy herds for their participation and support. We also thank Amber Hazel, El) Ballinger, Jennifer Belz, and Katie Konkol (of the University of Minnesota, St. Paul) for their technical assistance. NR 33 TC 14 Z9 15 U1 2 U2 25 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2012 VL 95 IS 5 BP 2697 EP 2702 DI 10.3168/jds.2011-5220 PG 6 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 929OJ UT WOS:000303074900046 PM 22541498 ER PT J AU Garcia-Peniche, TB Montaldo, HH Valencia-Posadas, M Wiggans, GR Hubbard, SM Torres-Vazquez, JA Shepard, L AF Garcia-Peniche, T. B. Montaldo, H. H. Valencia-Posadas, M. Wiggans, G. R. Hubbard, S. M. Torres-Vazquez, J. A. Shepard, L. TI Breed differences over time and heritability estimates for production and reproduction traits of dairy goats in the United States SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy goat breed; yield; reproduction; heritability ID MILK-YIELD; GENETIC-PARAMETERS; SHORT-COMMUNICATION; SAANEN GOATS; ANIMAL-MODEL; LACTATION; CHEESE; ALPINE; MEXICO; FAT AB To aid in improvement of breeding programs for production and reproduction traits of US dairy goats; breed differences over time were documented and genetic parameters were estimated. Data were from herds with >= 2 breeds (Alpine, LaMancha, Nubian; Oberhasli, Saanen; or Toggenburg), but only purebred data were analyzed. Three kidding periods were examined: 1976 through 1984; 1985 through 1994; and 1995 through 2005. Univariate repeatability mixed models were used to estimate least squares means by kidding period-breed and genetic parameters for milk; fat, and protein yields; combined fat and protein yield; fat and protein percentages; protein:fat ratio, age at first kidding, and kidding interval. Trends across kidding periods were favorable for most yield traits for all breeds but generally unfavorable for reproduction traits. Saanens had the highest milk (1;063 to 1,125 kg) and protein yields (31 to 33 kg). Nubians had the highest fat yields (37 to 40 kg) and lowest milk yields (791 to 851 kg). Oberhaslis had the lowest fat (31 to 33 kg) and protein (23 to 27 kg) yields. Alpines had the largest increase in milk yield (7.4%); Oberhaslis had the largest increase in protein (17.4%) and combined fat and protein (13.2%) yields. Combined fat and protein yield was higher for Nubians, Saanens; and Alpines (65 to 72 kg) than for LaManchas; Toggenburgs; and Oberhaslis (53 to 67 kg). Nubians had the highest fat (4.7 to 4.8%) and protein (3.6 to 3.8%) percentages. Only Nubians increased in fat percentage (2.1%); protein percentage increased most for Toggenburgs (7.4%) and Alpines (7.1%). Protein:fat ratio was highest for Toggenburgs (0.84 to 0.89) and lowest for Nubians (0.76 to 0.81); but Nubians had the largest increase in protein:fat ratio (6.6%). Saanens were oldest at first kidding (509 to 589 d), and Toggenburgs and LaManchas generally were youngest (435 to 545 d); age at first kidding increased most for Alpines (21.8%) and LaManchas (21.6%). Kidding intervals generally were shorter for Oberhaslis, LaManchas, and Nubians (350 to 377 d) than for Toggenburgs, Alpines, and Saanens (373 to 387 d). Kidding interval increased most for Nubians (3.9%) and Saanens (3.8%) and decreased only for Oberhaslis (5.4%). Heritability estimates across breeds were 0.35 for milk and fat yields, 0.37 for protein yield and protein:fat ratio, 0.36 for combined fat and protein yield, 0.52 for fat percentage, 0.54 for protein percentage, 0.23 for age at first kidding, and 0.05 for kidding interval. Genetic selection within breed is feasible for production and reproduction traits of US dairy goats. C1 [Montaldo, H. H.] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Genet & Bioestadist, Mexico City 04510, DF, Mexico. [Garcia-Peniche, T. B.] Inst Nacl Invest Forestales Agr & Pecuarias, Paso Del Toro 94277, Veracruz, Mexico. [Valencia-Posadas, M.] Univ Guanajuato, Div Ciencias Vida, Guanajuato 36500, Mexico. [Wiggans, G. R.; Hubbard, S. M.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. [Torres-Vazquez, J. A.] Inst Nacl Invest Forestales Agr & Pecuarias, Ctr Nacl Invest Fisiol & Mejoramiento Anim, Queretaro 76280, Mexico. [Shepard, L.] Amer Dairy Goat Assoc, Spindale, NC 28160 USA. RP Montaldo, HH (reprint author), Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Dept Genet & Bioestadist, Ciudad Univ, Mexico City 04510, DF, Mexico. EM montaldo@servidor.unam.mx NR 49 TC 9 Z9 10 U1 0 U2 15 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD MAY PY 2012 VL 95 IS 5 BP 2707 EP 2717 DI 10.3168/jds.2011-4714 PG 11 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 929OJ UT WOS:000303074900048 PM 22541500 ER PT J AU Augustine, DJ Derner, JD AF Augustine, David J. Derner, Justin D. TI Disturbance regimes and mountain plover habitat in shortgrass steppe: Large herbivore grazing does not substitute for prairie dog grazing or fire SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE black-tailed prairie dogs; Cynomys ludovicianus; ecosystem management; grassland birds; historical range of variation; livestock grazing; prescribed fire; rangeland management; spatial heterogeneity; vegetation structure ID NEST-SITE SELECTION; GREAT-PLAINS; ECOSYSTEM MANAGEMENT; CHARADRIUS-MONTANUS; PRESCRIBED FIRE; GRASSLAND; BIOLOGY; HETEROGENEITY; CONSERVATION; EVOLUTIONARY AB Restoring historical disturbance regimes to enhance habitat for grassland birds can conflict with livestock production goals and has been controversial because of uncertainty in the frequency and pattern of different disturbances prior to European settlement. We studied nesting habitat for the mountain plover (Charadrius montanus) in relation to prescribed fire, grazing by large herbivores (cattle), and grazing by black-tailed prairie dogs (Cynomys ludovicianus) in the shortgrass steppe of northeastern Colorado. Breeding mountain plovers primarily occurred on black-tailed prairie dog colonies or areas burned during the previous dormant season. Vegetation surrounding mountain plover nests and foraging locations was characterized by a fine-scale mosaic of prostrate (<4?cm tall) vegetated patches interspersed with >35% bare soil in a given square meter, with this fine-scale pattern distributed over a broad (>100-m radius) area. Mountain plovers rarely occupied grassland lacking prairie dogs or recent fire, but those that did selected sites with similar vegetation height and bare soil exposure as sites on burns and prairie dog colonies. Vegetation structure at mountain plover-occupied sites was also similar to random sites on burns and prairie dog colonies, but differed substantially from sites managed only with cattle. Intensive cattle grazing at twice the recommended stocking rate during spring (MarMay) or summer (MayOct) for 6 years produced significantly less bare soil than burns and prairie dog colonies, particularly following years with average or above-average precipitation. Thus, intensive cattle grazing did not substitute for prairie dog grazing or fire in terms of effects on vegetation structure and mountain plover habitat. Both prescribed burning and increased size and distribution of black-tailed prairie dog colonies appear to be effective and complementary means to manage for mountain plover breeding habitat in shortgrass steppe. Provision of mountain plover habitat has tradeoffs with traditional management for livestock production. Thus, managers need to clearly define desired outcomes for management to provide multiple ecosystem goods and services. (C) 2012 The Wildlife Society. C1 [Augustine, David J.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO 80526 USA. [Derner, Justin D.] ARS, Rangeland Resources Res Unit, USDA, Cheyenne, WY 82009 USA. RP Augustine, DJ (reprint author), ARS, Rangeland Resources Res Unit, USDA, 1701 Ctr Ave, Ft Collins, CO 80526 USA. EM david.augustine@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service (USDA-ARS) FX Funding for this study was provided by the United States Department of Agriculture, Agricultural Research Service (USDA-ARS). We thank P. McCusker for assistance with all aspects of data collection throughout the study. We thank Drs. Daniel Milchunas and Susan Skagen for helpful reviews of the manuscript, and the many technicians and students that assisted in the field or with photo analyses, including E. Hardy, D. Smith, J. Ling, T. Odell, J. Baker, S. Podolak, E. Klarquist, R. Moore, M. Ashby, J. Thomas, T. Smith, P. Freeman, M. Mortenson, and A. Whitman. We thank N. Clark, R. Reichert, B. Humphrey, and K. Philbrook for facilitating our work on Pawnee National Grassland. NR 49 TC 11 Z9 11 U1 4 U2 59 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2012 VL 76 IS 4 BP 721 EP 728 DI 10.1002/jwmg.334 PG 8 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 928QQ UT WOS:000302998300007 ER PT J AU Rose, AK Greenberg, CH Fearer, TM AF Rose, Anita K. Greenberg, Cathryn H. Fearer, Todd M. TI Acorn production prediction models for five common oak species of the eastern United States SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE acorn crop; acorn crop prediction; acorn production; acorns; hard mast; oak ID YIELDS; MOUNTAINS; PATTERNS AB Acorn production varies considerably among oak (Quercus) species, individual trees, years, and locations, which directly affects oak regeneration and populations of wildlife species that depend on acorns for food. Hard mast indices provide a relative ranking and basis for comparison of within- and between-year acorn crop size at a broad scale, but do not provide an estimate of actual acorn yieldthe number of acorns that can potentially be produced on a given land area unit based on the species, number, and diameter at breast height (dbh) of oak trees present. We used 10 years of acorn production data from 475 oak trees to develop predictive models of potential average annual hard mast production by five common eastern oak species, based on tree diameter and estimated crown area. We found a weak (R2?=?0.080.28) relationship between tree dbh and acorn production per unit crown area for most species. The relationship between tree dbh and acorn production per tree was stronger (R2?=?0.330.57). However, this is because larger-dbh trees generally have larger crowns, not because they have a greater capacity to produce more acorns per unit crown area. Acorn production is highly variable among individual trees. We estimated that dbh of at least 60 dominant or codominant oak trees per species should be randomly sampled to obtain an adequate representation of the range of dbhs (=12.7?cm dbh) in a given forest area, and achieve precise estimates when using these equations to predict potential acorn production. Our predictive models provide a tool for estimating potential acorn production that land managers and forest planners can apply to oak inventory data to tailor estimates of potential average annual acorn production to different forest management scenarios and multiple spatial scales. (C) 2011 The Wildlife Society. C1 [Rose, Anita K.] US Forest Serv, USDA, So Res Stn, Knoxville, TN 37919 USA. [Greenberg, Cathryn H.] US Forest Serv, USDA, So Res Stn, Asheville, NC 28806 USA. [Fearer, Todd M.] Amer Bird Conservancy, Appalachian Mt Joint Venture, Blacksburg, VA 24061 USA. RP Rose, AK (reprint author), US Forest Serv, USDA, So Res Stn, 4700 Old Kingston Pike, Knoxville, TN 37919 USA. EM anitarose@fs.fed.us FU United States Department of Agriculture Forest Service, Southern Research Station, Bent Creek Experimental Forest FX This study was funded by the United States Department of Agriculture Forest Service, Southern Research Station, Bent Creek Experimental Forest. Special thanks to T. Roof, K. Frick, J. Adams, and V. Gibbs for excellent project management and fieldwork and many other forestry technicians and volunteers for collecting and processing acorns. We thank the staff at the Pisgah National Forest, especially T. Oprean and J. Blanton for their assistance in finding study sites and continued logistical support. S. Zarnoch offered statistical advice and guidance. G. Warburton and R. Mumme helped to improve an earlier version of this manuscript. NR 43 TC 3 Z9 3 U1 2 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2012 VL 76 IS 4 BP 750 EP 758 DI 10.1002/jwmg.291 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 928QQ UT WOS:000302998300010 ER PT J AU Inman, RM Packila, ML Inman, KH Mccue, AJ White, GC Persson, J Aber, BC Orme, ML Alt, KL Cain, SL Fredrick, JA Oakleaf, BJ Sartorius, SS AF Inman, Robert M. Packila, Mark L. Inman, Kristine H. Mccue, Anthony J. White, Gary C. Persson, Jens Aber, Bryan C. Orme, Mark L. Alt, Kurt L. Cain, Steven L. Fredrick, Jay A. Oakleaf, Bob J. Sartorius, Shawn S. TI Spatial ecology of wolverines at the southern periphery of distribution SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE density; dispersal; Gulo gulo; home range; movement; niche; territory; wolverine; Yellowstone ID GREATER YELLOWSTONE ECOSYSTEM; CAPTURE-RECAPTURE MODELS; FREE-RANGING WOLVERINES; GULO-GULO; HOME-RANGE; UNITED-STATES; HABITAT USE; FEMALE WOLVERINE; BRITISH-COLUMBIA; POPULATION-SIZE AB Wolverines (Gulo gulo) in the conterminous United States have experienced range contraction, are uncommon, and have been designated as warranted for protection under the United States Endangered Species Act. Data from the southern edge of the wolverine's circumpolar distribution is sparse, and development of effective conservation strategies would benefit from a more complete understanding of the species' ecology. We captured and radio-monitored 30 wolverines in the Greater Yellowstone Ecosystem (GYE), tested for seasonal habitat selection by elevation band, and examined a suite of spatial characteristics to clarify our understanding of the wolverine's niche. Wolverines in GYE selected for areas >2,600?m latitude-adjusted elevation (LAE; n?=?2,257 wolverine locations [12 F, 6 M]). Wolverines avoided areas <2,150?m LAE, including during winter when the vast majority of ungulates are pushed to these elevations by deep snow. Wolverine home ranges were large relative to body size, averaging 303?km2 for adult females and 797?km2 for adult males (n?=?13 [8 F, 5 M] and 33 wolverine-years). Resident adults fit with Global Positioning System (GPS) collars used an area >75% the size of their multi-year home range in an average of 32 days (n?=?7 [5 F, 2 M]). Average movement rates of 1.3?km/2-hr indicated that both sexes move distances equivalent to the diameter of their home range every 2 days or the circumference of their home range in <1 week (n?=?1,329 2-hr movements, n?=?12 individuals [7 F, 5 M]). This capability for movement, the short time-frame over which home ranges were developed, and a lack of home range overlap by same sex adults (${\bar {x}} = 2.1\%\;{\rm overlap}$, 90% CI?=?0.04.8%, n?=?22 pairs) suggested territoriality. We estimated wolverine density to be 3.5/1,000?km2 of area >2,150?m LAE (95% CI?=?2.89.6). Dispersal movements extended to at least 170?km for both sexes (n?=?5 F, 2 M). At the southern edge of distribution, where suitable and unsuitable conditions exist in close proximity, wolverines selected high-elevation areas near alpine tree-line where a mix of forest, meadow, and boulder fields were present, deep snow-cover existed during winter, and low temperatures near freezing can occur throughout the year. Persistence in these areas where the growing season is brief requires large home ranges that are regularly patrolled, a social system that provides exclusive access to resources, and low densities. These characteristics, along with low reproductive rates, are prevalent throughout the species range, indicating that wolverines are specialists at exploiting a cold, unproductive niche where interspecific competition is limited. The vulnerability inherent in occupying this unproductive niche was likely influential in previous declines within the conterminous United States and will remain a factor as wolverines encounter modern human influences. Conserving wolverines in the conterminous United States will require collaborative management over a large geographic scale. (C) 2011 The Wildlife Society. C1 [Inman, Robert M.; Packila, Mark L.; Inman, Kristine H.; Mccue, Anthony J.] Swedish Univ Agr Sci, Wildlife Conservat Soc, Ennis, MT 59729 USA. [Inman, Robert M.] Swedish Univ Agr Sci, Grimso Wildlife Res Stn, Dept Ecol, Ennis, MT 59729 USA. [White, Gary C.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Persson, Jens] Swedish Univ Agr Sci, Dept Ecol, Grimso Wildlife Res Stn, SE-73091 Riddarhyttan, Sweden. [Aber, Bryan C.] US Forest Serv, Idaho Dept Fish & Game, Island Pk, ID 83429 USA. [Aber, Bryan C.] Wildlife Conservat Soc, Island Pk, ID 83429 USA. [Orme, Mark L.] US Forest Serv, Idaho Falls, ID 83401 USA. [Alt, Kurt L.] Montana Dept Fish Wildlife & Parks, Bozeman, MT 59718 USA. [Cain, Steven L.] Natl Pk Serv, Moose, WY 83012 USA. [Fredrick, Jay A.] US Forest Serv, Ennis, MT 59729 USA. [Oakleaf, Bob J.] Wyoming Game & Fish Dept, Lander, WY 82520 USA. [Sartorius, Shawn S.] US Fish & Wildlife Serv, Helena, MT 59601 USA. RP Inman, RM (reprint author), Swedish Univ Agr Sci, Wildlife Conservat Soc, 222 E Main St,Lone Elk Suite 3B, Ennis, MT 59729 USA. EM binman@wcs.org OI Persson, Jens/0000-0003-1405-7561 FU Beaverhead-Deerlodge and Bridger-Teton National Forests; Brainerd Foundation; Bullitt Foundation; Canyon Creek Foundation; Caribou-Targhee National Forest; Disney Worldwide Conservation Fund; Gallatin National Forest; Grand Teton National Park; Greater Yellowstone Coordinating Committee; Idaho Department of Fish and Game; Laura Moore Cunningham Foundation; Montana Department of Fish, Wildlife and Parks; National Fish and Wildlife Foundation; National Geographic Conservation Trust; New York Community Trust; Richard King Mellon Foundation; Tapeats Fund; Wildlife Conservation Society; Doris Duke Charitable Foundation; Wilburforce Foundation; Wyoming Game and Fish FX We thank the following for providing major funding, permits, or in-kind support: Beaverhead-Deerlodge and Bridger-Teton National Forests, Brainerd Foundation, Bullitt Foundation, Canyon Creek Foundation, Caribou-Targhee National Forest, Y. Chouinard, Disney Worldwide Conservation Fund, Gallatin National Forest, Grand Teton National Park, Greater Yellowstone Coordinating Committee, Idaho Department of Fish and Game, Laura Moore Cunningham Foundation, Montana Department of Fish, Wildlife and Parks, National Fish and Wildlife Foundation, National Geographic Conservation Trust, New York Community Trust, Richard King Mellon Foundation, Tapeats Fund, L. Westbrook, Wildlife Conservation Society Wildlife Action-Opportunities Fund supported by the Doris Duke Charitable Foundation, Wilburforce Foundation, Wyoming Game and Fish, and private individuals. We also thank J. Beecham, D. Betts, A. Chapin, D. Chapman, C. Dickinson, M. Hornocker, D. Horvath, G. Lust, J. Mattisson, F. McCarthy, D. McCauley, K. Pilgrim, K. Quigley, M. Schwartz, R. Spence, R. Wigglesworth, and numerous seasonal field biologists for their dedicated efforts. We appreciate the helpful comments of two anonymous reviewers. NR 91 TC 12 Z9 12 U1 11 U2 147 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2012 VL 76 IS 4 BP 778 EP 792 DI 10.1002/jwmg.289 PG 15 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 928QQ UT WOS:000302998300013 ER PT J AU Thompson, CM Royle, JA Garner, JD AF Thompson, Craig M. Royle, J. Andrew Garner, James D. TI A framework for inference about carnivore density from unstructured spatial sampling of scat using detector dogs SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE Bayesian; density; fisher; Martes pennanti; scat detector dogs; WinBUGS ID CAPTURE-RECAPTURE MODELS; MAXIMUM-LIKELIHOOD; POPULATION-SIZE; MARK-RECAPTURE; HIERARCHICAL MODEL; MARTES-PENNANTI; CAMERA-TRAP; PARAMETERS; MANAGEMENT; OCCUPANCY AB Wildlife management often hinges upon an accurate assessment of population density. Although undeniably useful, many of the traditional approaches to density estimation such as visual counts, livetrapping, or markrecapture suffer from a suite of methodological and analytical weaknesses. Rare, secretive, or highly mobile species exacerbate these problems through the reality of small sample sizes and movement on and off study sites. In response to these difficulties, there is growing interest in the use of non-invasive survey techniques, which provide the opportunity to collect larger samples with minimal increases in effort, as well as the application of analytical frameworks that are not reliant on large sample size arguments. One promising survey technique, the use of scat detecting dogs, offers a greatly enhanced probability of detection while at the same time generating new difficulties with respect to non-standard survey routes, variable search intensity, and the lack of a fixed survey point for characterizing non-detection. In order to account for these issues, we modified an existing spatially explicit, capturerecapture model for camera trap data to account for variable search intensity and the lack of fixed, georeferenced trap locations. We applied this modified model to a fisher (Martes pennanti) dataset from the Sierra National Forest, California, and compared the results (12.3?fishers/100?km2) to more traditional density estimates. We then evaluated model performance using simulations at 3 levels of population density. Simulation results indicated that estimates based on the posterior mode were relatively unbiased. We believe that this approach provides a flexible analytical framework for reconciling the inconsistencies between detector dog survey data and density estimation procedures. (C) 2011 The Wildlife Society. C1 [Thompson, Craig M.; Garner, James D.] US Forest Serv, USDA, Pacific SW Res Stn, Sierra Nevada Res Ctr, Fresno, CA 93710 USA. [Royle, J. Andrew] US Geol Survey, Patuxent Wildlife Res Ctr, Laurel, MD 20708 USA. RP Thompson, CM (reprint author), US Forest Serv, USDA, Pacific SW Res Stn, Sierra Nevada Res Ctr, Fresno, CA 93710 USA. EM cthompson@fs.fed.us OI Royle, Jeffrey/0000-0003-3135-2167 NR 57 TC 21 Z9 21 U1 7 U2 82 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD MAY PY 2012 VL 76 IS 4 BP 863 EP 871 DI 10.1002/jwmg.317 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 928QQ UT WOS:000302998300022 ER PT J AU Melnick, RL Testen, AL Poleatewich, AM Backman, PA Bailey, BA AF Melnick, R. L. Testen, A. L. Poleatewich, A. M. Backman, P. A. Bailey, B. A. TI Detection and expression of enterotoxin genes in endophytic strains of Bacillus cereus SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE Bacillus cereus; endophyte; enterotoxins; food-borne illness ID BIOLOGICAL-CONTROL AGENTS; CACAO; UW85 AB Aims: The aim of this study was to determine whether endophytic Bacillus cereus isolates from agronomic crops possessed genes for the nonhaemolytic enterotoxin (Nhe) and haemolysin BL (HBL) and, therefore, have the potential to cause diarrhoeal illness in humans. Methods and Results: PCR followed by sequencing confirmed the presence of enterotoxin genes nheA, nheB, nheC, hblA, hblC, hblD in endophytic B. cereus. All nhe genes were detected in 59% of endophytic B. cereus, while all hbl genes were detected in 44%. All six genes were detected in 41% of isolates. Enterotoxin genes were not detected in 15% of B. cereus isolates. Reverse transcriptase real-time PCR confirmed that endophytic B. cereus could express enterotoxin genes in pure culture. Conclusions: This study showed that endophytic B. cereus isolates that possess genes for enterotoxin production are present in agronomic crops. Other endophytic B. cereus isolates lacked specific genes or lacked all nhe and hbl genes. Additionally, host, country of origin and tissue of origin had no impact on the enterotoxin genes detected. Significance and Impact of the Study: Bacillus cereus with the potential of causing diarrhoeal illness in humans is a cosmopolitan endophytic inhabitant of plants, not incidental surface inhabitants or contaminants, as often suggested by previous research. C1 [Melnick, R. L.; Bailey, B. A.] USDA ARS, Sustainable Perennial Crops Lab, Beltsville, MD 20705 USA. [Melnick, R. L.; Testen, A. L.; Poleatewich, A. M.; Backman, P. A.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. RP Melnick, RL (reprint author), USDA ARS, Sustainable Perennial Crops Lab, 10300 Baltimore Ave,BARC W BLDG 001, Beltsville, MD 20705 USA. EM rachel.melnick@ars.usda.gov RI Melnick, Rachel/B-1719-2013 OI Melnick, Rachel/0000-0002-5113-2551 FU USAID's IPM-CRSP; SANREM-CRSP FX USDA is an equal opportunity provider and employer. This work was supported by USAID's IPM-CRSP and SANREM-CRSP. We thank Dr S. Doores, Department of Food Science at The Pennsylvania State University for her critical review of the manuscript. NR 17 TC 3 Z9 3 U1 0 U2 15 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0266-8254 J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD MAY PY 2012 VL 54 IS 5 BP 468 EP 474 DI 10.1111/j.1472-765X.2012.03232.x PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 923JM UT WOS:000302615600014 PM 22394121 ER PT J AU Hershkovitz, V Ben-Dayan, C Raphael, G Pasmanik-Chor, M Liu, J Belausov, E Aly, R Wisniewski, M Droby, S AF Hershkovitz, Vera Ben-Dayan, Clarita Raphael, Ginat Pasmanik-Chor, Metsada Liu, Jia Belausov, Eduard Aly, Radi Wisniewski, Michael Droby, Samir TI Global changes in gene expression of grapefruit peel tissue in response to the yeast biocontrol agent Metschnikowia fructicola SO MOLECULAR PLANT PATHOLOGY LA English DT Article ID DEFENSE-RELATED PROTEINS; PENICILLIUM-DIGITATUM; DISEASE RESISTANCE; CITRUS-FRUIT; CRYPTOCOCCUS-LAURENTII; BIOLOGICAL-CONTROL; BOTRYTIS-CINEREA; SALICYLIC-ACID; APPLE FRUIT; HYDROGEN-PEROXIDE AB To gain a better understanding of the molecular changes taking place in citrus fruit tissue following the application of the yeast biocontrol agent Metschnikowia fructicola, microarray analysis was performed on grapefruit surface wounds using an Affymetrix Citrus GeneChip. Using a cut-off of P < 0.05 and a 1.5-fold change difference as biologically significant, the data indicated that 1007 putative unigenes showed significant expression changes following wounding and yeast application relative to wounded controls. Microarray results of selected genes were validated by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). The data indicated that yeast application induced the expression of the genes encoding Respiratory burst oxidase (Rbo), mitogen-activated protein kinase (MAPK) and mitogen-activated protein kinase kinase (MAPKK), G-proteins, chitinase (CHI), phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS) and 4-coumarate-CoA ligase (4CL). In contrast, three genes, peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT), were down-regulated in grapefruit peel tissue treated with yeast cells. Moreover, suppression was correlated with significantly higher levels of hydrogen peroxide, superoxide anion and hydroxyl radical production in yeast-treated surface wounds. Interestingly, large amounts of hydrogen peroxide were detected inside yeast cells recovered from wounded fruit tissue, indicating the ability of the yeast to activate reactive oxygen species when it is in contact with plant tissue. This study provides the first global picture of gene expression changes in grapefruit in response to the yeast antagonist M. fructicola. C1 [Hershkovitz, Vera; Ben-Dayan, Clarita; Raphael, Ginat; Belausov, Eduard; Droby, Samir] Agr Res Org, Dept Postharvest Sci, Volcani Ctr, IL-50250 Bet Dagan, Israel. [Hershkovitz, Vera; Ben-Dayan, Clarita] Hebrew Univ Jerusalem, Robert H Smith Fac Agr Food & Environm, IL-76100 Rehovot, Israel. [Pasmanik-Chor, Metsada] Tel Aviv Univ, Bioinformat Unit, Fac Life Sci, IL-69978 Tel Aviv, Israel. [Liu, Jia; Wisniewski, Michael] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Droby, S (reprint author), Agr Res Org, Dept Postharvest Sci, Volcani Ctr, POB 6, IL-50250 Bet Dagan, Israel. EM samird@volcani.agri.gov.il FU US-Israel Binational Agricultural Research and Development (BARD) [IS-4268-09] FX This research was supported in part by a grant (IS-4268-09) from the US-Israel Binational Agricultural Research and Development (BARD) Fund to Samir Droby and Michael Wisniewski. NR 48 TC 25 Z9 27 U1 5 U2 29 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1464-6722 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD MAY PY 2012 VL 13 IS 4 BP 338 EP 349 DI 10.1111/j.1364-3703.2011.00750.x PG 12 WC Plant Sciences SC Plant Sciences GA 921HW UT WOS:000302469700002 PM 22017757 ER PT J AU Niu, SL Luo, YQ Fei, SF Yuan, WP Schimel, D Law, BE Ammann, C Arain, MA Arneth, A Aubinet, M Barr, A Beringer, J Bernhofer, C Black, TA Buchmann, N Cescatti, A Chen, JQ Davis, KJ Dellwik, E Desai, AR Etzold, S Francois, L Gianelle, D Gielen, B Goldstein, A Groenendijk, M Gu, LH Hanan, N Helfter, C Hirano, T Hollinger, DY Jones, MB Kiely, G Kolb, TE Kutsch, WL Lafleur, P Lawrence, DM Li, LH Lindroth, A Litvak, M Loustau, D Lund, M Marek, M Martin, TA Matteucci, G Migliavacca, M Montagnani, L Moors, E Munger, JW Noormets, A Oechel, W Olejnik, J Kyaw, TPU Pilegaard, K Rambal, S Raschi, A Scott, RL Seufert, G Spano, D Stoy, P Sutton, MA Varlagin, A Vesala, T Weng, ES Wohlfahrt, G Yang, B Zhang, ZD Zhou, XH AF Niu, Shuli Luo, Yiqi Fei, Shenfeng Yuan, Wenping Schimel, David Law, Beverly E. Ammann, Christof Arain, M. Altaf Arneth, Almut Aubinet, Marc Barr, Alan Beringer, Jason Bernhofer, Christian Black, T. Andrew Buchmann, Nina Cescatti, Alessandro Chen, Jiquan Davis, Kenneth J. Dellwik, Ebba Desai, Ankur R. Etzold, Sophia Francois, Louis Gianelle, Damiano Gielen, Bert Goldstein, Allen Groenendijk, Margriet Gu, Lianhong Hanan, Niall Helfter, Carole Hirano, Takashi Hollinger, David Y. Jones, Mike B. Kiely, Gerard Kolb, Thomas E. Kutsch, Werner L. Lafleur, Peter Lawrence, David M. Li, Linghao Lindroth, Anders Litvak, Marcy Loustau, Denis Lund, Magnus Marek, Michal Martin, Timothy A. Matteucci, Giorgio Migliavacca, Mirco Montagnani, Leonardo Moors, Eddy Munger, J. William Noormets, Asko Oechel, Walter Olejnik, Janusz Kyaw Tha Paw U Pilegaard, Kim Rambal, Serge Raschi, Antonio Scott, Russell L. Seufert, Guenther Spano, Donatella Stoy, Paul Sutton, Mark A. Varlagin, Andrej Vesala, Timo Weng, Ensheng Wohlfahrt, Georg Yang, Bai Zhang, Zhongda Zhou, Xuhui TI Thermal optimality of net ecosystem exchange of carbon dioxide and underlying mechanisms SO NEW PHYTOLOGIST LA English DT Article DE climate change; optimum temperature; temperature acclimation; temperature adaptation; thermal optimality ID TEMPERATURE-DEPENDENCE; SOIL RESPIRATION; EUROPEAN FORESTS; CYCLE FEEDBACK; PONDEROSA PINE; CO2 FLUXES; CLIMATE; PHOTOSYNTHESIS; ACCLIMATION; RESPONSES AB It is well established that individual organisms can acclimate and adapt to temperature to optimize their functioning. However, thermal optimization of ecosystems, as an assemblage of organisms, has not been examined at broad spatial and temporal scales. Here, we compiled data from 169 globally distributed sites of eddy covariance and quantified the temperature response functions of net ecosystem exchange (NEE), an ecosystem-level property, to determine whether NEE shows thermal optimality and to explore the underlying mechanisms. We found that the temperature response of NEE followed a peak curve, with the optimum temperature (corresponding to the maximum magnitude of NEE) being positively correlated with annual mean temperature over years and across sites. Shifts of the optimum temperature of NEE were mostly a result of temperature acclimation of gross primary productivity (upward shift of optimum temperature) rather than changes in the temperature sensitivity of ecosystem respiration. Ecosystem-level thermal optimality is a newly revealed ecosystem property, presumably reflecting associated evolutionary adaptation of organisms within ecosystems, and has the potential to significantly regulate ecosystemclimate change feedbacks. The thermal optimality of NEE has implications for understanding fundamental properties of ecosystems in changing environments and benchmarking global models. C1 [Niu, Shuli; Luo, Yiqi; Fei, Shenfeng; Weng, Ensheng; Zhang, Zhongda] Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA. [Luo, Yiqi; Zhou, Xuhui] Fudan Univ, Inst Global Environm Change Res, Shanghai 200433, Peoples R China. [Yuan, Wenping] Beijing Normal Univ, Coll Global Change & Earth Syst Sci, Beijing 100875, Peoples R China. [Schimel, David] NEON Inc, Boulder, CO 80301 USA. [Law, Beverly E.] Oregon State Univ, Coll Forestry, Corvallis, OR 97331 USA. [Ammann, Christof] Fed Res Stn Agroscope Reckenholz Tanikon, CH-8046 Zurich, Switzerland. [Arain, M. Altaf] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON L8S 4K1, Canada. [Arneth, Almut; Lindroth, Anders; Lund, Magnus] Lund Univ, Dept Phys Geog & Ecosyst Anal, S-22362 Lund, Sweden. [Arneth, Almut] Karlsruhe Inst Technol, Inst Meteorol & Climate Res, Karlsruhe, Germany. [Aubinet, Marc] Fac Univ Sci Agronom Gembloux, Unite Phys Biosyst, B-5030 Gembloux, Belgium. [Barr, Alan] Environm Canada, Div Climate Res, Saskatoon, SK S7N 3H5, Canada. [Beringer, Jason] Monash Univ, Sch Geog & Environm Sci, Clayton, Vic 3800, Australia. [Bernhofer, Christian] Tech Univ Dresden, Inst Hydrol & Meteorol, Chair Meteorol, D-01062 Dresden, Germany. [Black, T. Andrew] Univ British Columbia, Vancouver, BC V6T 1Z4, Canada. [Buchmann, Nina; Etzold, Sophia] ETH, Inst Plant Sci, CH-8092 Zurich, Switzerland. [Cescatti, Alessandro; Migliavacca, Mirco; Seufert, Guenther] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, Ispra, Italy. [Chen, Jiquan] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Davis, Kenneth J.] Penn State Univ, Ctr Earth Syst Sci, State Coll, PA 16802 USA. [Dellwik, Ebba] Tech Univ Denmark, Riso Natl Lab Sustainable Energy, Wind Energy Div, DK-4000 Roskilde, Denmark. [Desai, Ankur R.] Univ Wisconsin, Dept Atmospher & Ocean Sci, Madison, WI 53706 USA. [Francois, Louis] Univ Liege, UMCCB, B-4000 Liege, Belgium. [Gianelle, Damiano] Fdn Edmund Mach, IASMA Res & Innovat Ctr, Sustainable Agro Ecosyst & Bioresources Dept, I-38010 San Michele All Adige, TN, Italy. [Gielen, Bert] Univ Antwerp, Dept Biol, B-2610 Antwerp, Belgium. [Goldstein, Allen] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Groenendijk, Margriet] Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Earth Sci, NL-1081 HV Amsterdam, Netherlands. [Gu, Lianhong; Yang, Bai] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Hanan, Niall] S Dakota State Univ, Geog Informat Sci Ctr Excellence GIScCE, Brookings, SD 57007 USA. [Helfter, Carole] CEH, Penicuik EH26 0QB, Midlothian, Scotland. [Hirano, Takashi] Hokkaido Univ N9, Kita Ku, Sapporo, Hokkaido 0608589, Japan. [Hollinger, David Y.; Sutton, Mark A.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Jones, Mike B.] Trinity Coll Dublin, Dept Bot, Dublin, Ireland. [Kiely, Gerard] Natl Univ Ireland Univ Coll Cork, Civil & Environm Engn Dept, Cork, Ireland. [Kolb, Thomas E.] No Arizona Univ, Sch Forestry, Flagstaff, AZ 86001 USA. [Kutsch, Werner L.] Johann Heinrich von Thunen Inst vTI, Inst Climate Res, Braunschweig, Germany. [Lafleur, Peter] Trent Univ, Dept Geog, Peterborough, ON K9J 7B8, Canada. [Lawrence, David M.] Natl Ctr Atmospher Res, Boulder, CO 80305 USA. [Li, Linghao] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Changes, Beijing 100864, Peoples R China. [Litvak, Marcy] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Loustau, Denis] INRA, EPHYSE UR1263, F-33140 Villenave Dornon, France. [Marek, Michal] Acad Sci Czech Republic, Inst Syst Biol & Ecol, CZ-60300 Brno, Czech Republic. [Martin, Timothy A.] Univ Florida, Gainesville, FL 32611 USA. [Matteucci, Giorgio] CNR, Inst Agroenvironm & Forest Biol, I-00015 Monterotondo, RM, Italy. [Montagnani, Leonardo] Agenzia Ambiente, Serv Forestali, I-39100 Bolzano, Provincia Auton, Italy. [Montagnani, Leonardo] Free Univ Bolzano, Fac Sci & Technol, I-39100 Bolzano, Italy. [Moors, Eddy] Wageningen UR, ESS CC, NL-6700 AA Wageningen, Netherlands. [Munger, J. William] Harvard Univ, Dept Earth & Planetary Sci, Cambridge, MA 02138 USA. [Noormets, Asko] N Carolina State Univ, US Forest Serv, USDA, So Global Change Program, Raleigh, NC 27606 USA. [Oechel, Walter] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA. [Olejnik, Janusz] PULS, Dept Meteorol, PL-60667 Poznan, Poland. [Kyaw Tha Paw U] Univ Calif Davis, LAWR, Atmospher Sci Grp, Davis, CA 95616 USA. [Pilegaard, Kim] Tech Univ Denmark, Riso Natl Lab Sustainable Energy, Biosyst Div, DK-4000 Roskilde, Denmark. [Rambal, Serge] CNRS, DREAM, CEFE, UMR5175, F-34293 Montpellier 5, France. [Raschi, Antonio] CNR Inst Biometeorol IBIMET, I-50145 Florence, Italy. [Scott, Russell L.] ARS, USDA, SW Watershed Res Ctr, Tucson, AZ 85719 USA. [Spano, Donatella] Univ Sassari, Dept Econ & Woody Plant Ecosyst, I-07100 Sassari, Italy. [Stoy, Paul] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Varlagin, Andrej] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow 119071, Russia. [Vesala, Timo] Univ Helsinki, Dept Phys, FI-00014 Helsinki, Finland. [Wohlfahrt, Georg] Univ Innsbruck, Inst Ecol, A-6020 Innsbruck, Austria. RP Niu, SL (reprint author), Univ Oklahoma, Dept Bot & Microbiol, Norman, OK 73019 USA. EM sniu@ou.edu RI Munger, J/H-4502-2013; li, wenchao/S-5567-2016; Gu, Lianhong/H-8241-2014; Vesala, Timo/C-3795-2017; Beringer, Jason/B-8528-2008; Law, Beverly/G-3882-2010; Lund, Magnus/J-4922-2013; Goldstein, Allen/A-6857-2011; Oechel, Walter/F-9361-2010; Lindroth, Anders/N-4697-2014; Wohlfahrt, Georg/D-2409-2009; Marek, Michal V./D-4383-2014; Desai, Ankur/A-5899-2008; Gianelle, Damiano/G-9437-2011; Barr, Alan/H-9939-2014; Seufert, Gunther/J-9918-2013; Zhou, Xuhui/H-4332-2011; Matteucci, Giorgio/N-3526-2015; Montagnani, Leonardo/F-1837-2016; Chen, Jiquan/D-1955-2009; Weng, Ensheng/E-4390-2012; Hollinger, David/G-7185-2012; Migliavacca, mirco/C-1260-2011; Moors, Eddy/J-5165-2012; Yuan, Wu/E-8847-2010; Sutton, Mark/K-2700-2012; Buchmann, Nina/E-6095-2011; Arneth, Almut/B-2702-2013; Hirano, Takashi/A-4557-2012; Lawrence, David/C-4026-2011; Pilegaard, Kim/I-7137-2013; Kiely, Gerard/I-8158-2013 OI Hanan, Niall/0000-0002-9130-5306; Noormets, Asko/0000-0003-2221-2111; Kiely, Gerard/0000-0003-2189-6427; Martin, Timothy/0000-0002-7872-4194; Munger, J/0000-0002-1042-8452; Gu, Lianhong/0000-0001-5756-8738; Vesala, Timo/0000-0002-4852-7464; Beringer, Jason/0000-0002-4619-8361; Law, Beverly/0000-0002-1605-1203; rambal, serge/0000-0001-5869-8382; Arain, M. Altaf/0000-0002-1433-5173; Lund, Magnus/0000-0003-1622-2305; Goldstein, Allen/0000-0003-4014-4896; Oechel, Walter/0000-0002-3504-026X; Lindroth, Anders/0000-0002-7669-784X; Wohlfahrt, Georg/0000-0003-3080-6702; Desai, Ankur/0000-0002-5226-6041; Gianelle, Damiano/0000-0001-7697-5793; Seufert, Gunther/0000-0002-6019-6688; Matteucci, Giorgio/0000-0002-4790-9540; Montagnani, Leonardo/0000-0003-2957-9071; Weng, Ensheng/0000-0002-1858-4847; Moors, Eddy/0000-0003-2309-2887; Lawrence, David/0000-0002-2968-3023; Pilegaard, Kim/0000-0002-5169-5717; FU Office of Science, US Department of Energy [DE-FG02-006ER64317]; US National Science Foundation (NSF) [DEB 0444518, DEB 0743778, DEB 0840964, DBI 0850290, EPS 0919466]; CFCAS; NSERC; BIOCAP; Environment Canada; NRCan; Office of Science US Department of Energy for AmeriFlux; CarboEuropeIP; FAP-GTOS-TCO; iLEAPS; NitroEurope; Max Planck Institute for Biogeochemistry; National Science Foundation; University of Tuscia; Universite Laval and Environment Canada FX This work was financially supported by the Terrestrial Carbon Program at the Office of Science, US Department of Energy, grants DE-FG02-006ER64317 and the US National Science Foundation (NSF), grant DEB 0444518, DEB 0743778, DEB 0840964, DBI 0850290, and EPS 0919466 to Y.L. We used the eddy covariance data acquired by the FLUXNET community and, in particular, by the following networks: AmeriFlux (US Department of Energy, Biological and Environmental Research, Terrestrial Carbon Program (DE-FG02-04ER63917 and DE-FG02-04ER63911)), GHG-Europe, Fluxnet-Canada Research Network and Canadian Carbon Program (supported by CFCAS, NSERC, BIOCAP, Environment Canada, and NRCan), GreenGrass, KoFlux, LBA, NECC, OzFlux, TCOS-Siberia, and USCCC. We acknowledge the financial support to the eddy covariance data harmonization provided by the Office of Science US Department of Energy for AmeriFlux, CarboEuropeIP, FAP-GTOS-TCO, iLEAPS, NitroEurope, Max Planck Institute for Biogeochemistry, National Science Foundation, University of Tuscia, Universite Laval and Environment Canada, and database development and technical support from the Berkeley Water Center, Lawrence Berkeley National Laboratory, and Microsoft Research eScience. NR 42 TC 27 Z9 28 U1 3 U2 139 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X J9 NEW PHYTOL JI New Phytol. PD MAY PY 2012 VL 194 IS 3 BP 775 EP 783 DI 10.1111/j.1469-8137.2012.04095.x PG 9 WC Plant Sciences SC Plant Sciences GA 923KS UT WOS:000302618800019 PM 22404566 ER PT J AU Charles, RA Kjos, S Ellis, AE Dubey, JP Shock, BC Yabsley, MJ AF Charles, Roxanne A. Kjos, Sonia Ellis, Angela E. Dubey, J. P. Shock, Barbara C. Yabsley, Michael J. TI Parasites and vector-borne pathogens of southern plains woodrats (Neotoma micropus) from southern Texas SO PARASITOLOGY RESEARCH LA English DT Article ID TAENIA-TAENIAEFORMIS; TOXOPLASMA-GONDII; LEISHMANIA-MEXICANA; BESNOITIA-JELLISONI; CUTEREBRA AUSTENI; EASTERN WOODRAT; UNITED-STATES; PREVALENCE; INFECTION; RODENTS AB From 2008 to 2010, southern plains woodrats (Neotoma micropus) from southern Texas, were examined for parasites and selected pathogens. Eight helminth species were recovered from 97 woodrats including, Trichuris neotomae from 78 (prevalence = 80%), Ascarops sp. from 42 (43%), Nematodirus neotoma from 31 (32%), Raillietina sp. from nine (9%), Taenia taeniaeformis larvae from eight (8%), and an unidentified spiurid, a Scaphiostomum sp. and a Zonorchis sp. each from a single woodrat. Besnotia neotomofelis was detected in three (3%) woodrats and microfilaria were detected in seven (7%). Polymerase chain reaction (PCR) testing of blood samples from 104 woodrats detected a novel Babesia sp. in one (1%) and Hepatozoon sp. in 17 (16%) woodrats. Partial 18S rRNA gene sequence of the Babesia was 94% similar to B. conradae. Histologic examination of tissues detected intestinal coccidia in seven of 104 (7%), Sarcocystis neotomafelis in 26 (25%), Hepatozoon sp. in 21 (20%), and Dunnifilaria meningica in four (4%) woodrats. Three woodrats (5%) were seropositive for Toxoplasma gondii. Ectoparasites recovered included fleas (Orchopeas sexdentatus and O. neotomae), ticks (Ixodes woodi and Ornithodoros turicata), mites (Trombicula sp. and Ornithonyssus (Bdellonyssus) bacoti) and bot flies (Cuterebra sp.). The only difference in prevalence related to gender was for N. neotoma (males > females, p = 0.029). Prevalence of T. neotomae and all intestinal parasites combined was significantly higher in adults compared with juveniles (p = 0.0068 and p = 0.0004), respectively. Lesions or clinical signs were associated with Cuterebra and B. neotomofelis. Collectively, these data indicate that woodrats from southern Texas harbor several parasites of veterinary and/or medical importance. C1 [Shock, Barbara C.; Yabsley, Michael J.] Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. [Charles, Roxanne A.; Shock, Barbara C.; Yabsley, Michael J.] Univ Georgia, Coll Vet Med, Dept Populat Hlth, SE Cooperat Wildlife Dis Study, Athens, GA 30602 USA. [Kjos, Sonia] Marshfield Med Res Fdn, Marshfield, WI 54449 USA. [Ellis, Angela E.] Univ Georgia, Coll Vet Med, Athens Diagnost Lab, Athens, GA 30602 USA. [Dubey, J. P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Yabsley, MJ (reprint author), Univ Georgia, Daniel B Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. EM myabsley@uga.edu FU National Institutes of Health [R15 AI067304-02]; Fulbright (LASPAU); SCWDS FX The authors thank J. Edwards, E. Blizzard, and D. Roellig for field assistance and L. Saunders, A. Page, and J. Huang for laboratory assistance. We also thank several landowners who generously allowed us to sample animals on their properties. This study was primarily supported by the National Institutes of Health (R15 AI067304-02) which was focused on understanding the ecology of Trypanosoma cruzi in these woodrats. Additional support was provided by the Southeastern Cooperative Wildlife Disease Study (SCWDS) (http://scwds.org/), through continued sponsorship of SCWDS member state and federal agencies. RAC was supported by the Fulbright (LASPAU) program. NR 53 TC 2 Z9 3 U1 2 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 J9 PARASITOL RES JI Parasitol. Res. PD MAY PY 2012 VL 110 IS 5 BP 1855 EP 1862 DI 10.1007/s00436-011-2710-z PG 8 WC Parasitology SC Parasitology GA 926ES UT WOS:000302814700033 PM 22108764 ER PT J AU Santin, M Dargatz, D Fayer, R AF Santin, Monica Dargatz, David Fayer, Ronald TI Prevalence and genotypes of Enterocytozoon bieneusi in weaned beef calves on cow-calf operations in the USA SO PARASITOLOGY RESEARCH LA English DT Article ID VIRUS-INFECTED PATIENTS; EASTERN UNITED-STATES; INTESTINAL MICROSPORIDIOSIS; 1ST DETECTION; MOLECULAR CHARACTERIZATION; ENCEPHALITOZOON-CUNICULI; DAIRY CALVES; PIG FARM; IDENTIFICATION; DIARRHEA AB To determine the prevalence and genotype distribution of Enterocytozoon bieneusi in weaned beef calves in the USA, fecal samples were collected from 819 calves (6-18 months of age) from 49 operations. Feces were sieved and subjected to density gradient centrifugation to remove fecal debris and to concentrate spores. DNA extracted from each sample was subjected to the polymerase chain reaction (PCR) to amplify the complete internal transcriber spacer (ITS). All PCR-positive specimens were sequenced to determine the genotype(s) present. Overall, E. bieneusi was detected in 34.8% of the 819 fecal samples. The highest prevalence was found in the Midwest region (42.7%) followed by the South (35.8%) and the West (23.2%). The prevalence of E. bieneusi varied considerably from operation to operation (0-100%). A prevalence of 100% was observed in three operations, one in the Midwest and two in the South; E. bieneusi was not found in six operations, three in the South and three in the West. Sequence analysis revealed the presence of six genotypes, four previously reported (I, J, BEB4, and type IV) and two novel genotypes (BEB8 and BEB9). Mixed infections were identified in five specimens, three contained I and BEB4 and two contained J and BEB4. Most of the positive calves (238 of 285) harbored genotypes with zoonotic potential including I (59), J (108), BEB4 (65), type IV (1), mixed I/BEB4 (3), and mixed J/BEB4 (2). C1 [Santin, Monica; Fayer, Ronald] ARS, USDA, Environm Microbial & Food Safety Lab, Anim & Nat Resources Inst, Beltsville, MD 20705 USA. [Dargatz, David] Anim & Plant Hlth Inspect Serv, USDA, Ctr Epidemiol, Ft Collins, CO 80526 USA. [Dargatz, David] Anim & Plant Hlth Inspect Serv, USDA, Ctr Anim Hlth, Ft Collins, CO 80526 USA. RP Santin, M (reprint author), ARS, USDA, Environm Microbial & Food Safety Lab, Anim & Nat Resources Inst, Bldg 173,BARC E,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM monica.santin-duran@ars.usda.gov NR 57 TC 19 Z9 20 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 J9 PARASITOL RES JI Parasitol. Res. PD MAY PY 2012 VL 110 IS 5 BP 2033 EP 2041 DI 10.1007/s00436-011-2732-6 PG 9 WC Parasitology SC Parasitology GA 926ES UT WOS:000302814700053 PM 22146995 ER PT J AU Crook, AD Friesen, TL Liu, ZH Ojiambo, PS Cowger, C AF Crook, A. D. Friesen, T. L. Liu, Z. H. Ojiambo, P. S. Cowger, C. TI Novel Necrotrophic Effectors from Stagonospora nodorum and Corresponding Host Sensitivities in Winter Wheat Germplasm in the Southeastern United States SO PHYTOPATHOLOGY LA English DT Article ID SELECTIVE TOXINS; SEPTORIA-NODORUM; TAN SPOT; SEEDLING RESISTANCE; GENE; DISEASE; BLOTCH; LEAF; SUSCEPTIBILITY; PATHOSYSTEM AB Stagonospora nodorum blotch (SNB), caused by the necrotrophic fungus Stagonospora nodorum (teleomorph: Phaeosphaeria nodorum), is among the most common diseases of winter wheat in the United States. New opportunities in resistance breeding have arisen from the recent discovery of several necrotrophic effectors (NEs, also known as host-selective toxins) produced by S. nodorum, along with their corresponding host sensitivity (Snn) genes. Thirty-nine isolates of S. nodorum collected from wheat debris or grain from seven states in the southeastern United States were used to investigate the production of NEs in the region. Twenty-nine cultivars with varying levels of resistance to SNB, representing 10 eastern-U.S. breeding programs, were infiltrated with culture filtrates from the S. nodorum isolates in a randomized complete block design. Three single-NE Pichia pastoris controls, two S. nodorum isolate controls, and six Snn-differential wheat controls were also used. Cultivar-isolate interactions were visually evaluated for sensitivity at 7 days after infiltration. Production of NEs was detected in isolates originating in each sampled state except Maryland. Of the 39 isolates, 17 produced NEs different from those previously characterized in the upper Great Plains region. These novel NEs likely correspond to unidentified Snn genes in Southeastern wheat cultivars, because NEs are thought to arise under selection pressure from genes for resistance to biotrophic pathogens of wheat cultivars that differ by geographic region. Only 3, 0, and 23% of the 39 isolates produced SnToxA, SnTox1, and SnTox3, respectively, by the culture-filtrate test. A Southern dot-blot test showed that 15, 74, and 39% of the isolates carried the genes for those NEs, respectively; those percentages were lower than those found previously in larger international samples. Only two cultivars appeared to contain known Snn genes, although half of the cultivars displayed sensitivity to culture filtrates containing unknown NEs. Effector sensitivity was more frequent in SNB-susceptible cultivars than in moderately resistant (MR) cultivars (P == 0.008), although some susceptible cultivars did not exhibit sensitivity to NEs produced by isolates in this study and some MR cultivars were sensitive to NEs of multiple isolates. Our results suggest that NE sensitivities influence but may not be the only determinant of cultivar resistance to S. nodorum. Specific knowledge of NE and Snn gene frequencies in this region can be used by wheat breeding programs to improve SNB resistance. C1 [Cowger, C.] N Carolina State Univ, USDA, ARS, Dept Plant Pathol, Raleigh, NC 27695 USA. [Crook, A. D.; Ojiambo, P. S.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Friesen, T. L.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58105 USA. [Liu, Z. H.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. RP Cowger, C (reprint author), N Carolina State Univ, USDA, ARS, Dept Plant Pathol, Raleigh, NC 27695 USA. EM Christina.Cowger@ars.usda.gov NR 31 TC 7 Z9 7 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2012 VL 102 IS 5 BP 498 EP 505 DI 10.1094/PHYTO-08-11-0238 PG 8 WC Plant Sciences SC Plant Sciences GA 927ZW UT WOS:000302950300006 PM 22494247 ER PT J AU Liu, ZH Zhong, S Stasko, AK Edwards, MC Friesen, TL AF Liu, Z. H. Zhong, S. Stasko, A. K. Edwards, M. C. Friesen, T. L. TI Virulence Profile and Genetic Structure of a North Dakota Population of Pyrenophora teres f. teres, the Causal Agent of Net Form Net Blotch of Barley SO PHYTOPATHOLOGY LA English DT Article DE spot form; virulence diversity ID MATING-TYPE LOCUS; DRECHSLERA-TERES; PATHOGENIC VARIATION; LINKAGE DISEQUILIBRIUM; RAPD MARKERS; MAJOR GENES; DIVERSITY; RESISTANCE; IDENTIFICATION; MACULATA AB A Pyrenophora teres f. teres population in North Dakota was analyzed for virulence variation and genetic diversity using 75 monospore isolates that were collected across a 4-year period (2004 to 2007) from two North Dakota State University agricultural experiment stations at Fargo and Langdon. Pathogenicity tests by inoculation onto 22 barley differential lines at seedling stage revealed 49 pathotypes, indicating a wide range of pathogenic diversity. Two-way analysis of variance of disease ratings revealed a significant difference in the virulence among isolates and in the resistance among barley lines, as well as in the interactions between the two. 'CI5791', 'Algerian', and 'Heartland' were three barley lines showing a high level of seedling resistance to all North Dakota isolates tested; however, many previously reported resistance genes have been overcome. Forty multilocus genotypes were identified from this set of isolates by genotyping at 13 simple-sequence repeat loci. High percentages of clonal cultures were detected in the samplings from 2005 and 2007 in Fargo and 2005 in Langdon. Using a clone-corrected sample set, the mean gene diversity (h) was estimated to be 0.58, approximately the same for both locations. The calculated Wright's FST value is small (0.11) but was significantly > 0, indicating a significant differentiation between the Fargo and Langdon populations. In the gametic disequilibrium test, only 3 of 78 possible pairwise comparisons over all isolates showed significant (P < 0.05) nonrandom association, suggesting a random mating mode. Our results suggest that the populations from the two locations are derived from a common source and undergo frequent recombination. This research provides important information for barley breeders regarding development and deployment of cultivars with resistance to net form net blotch in this region. C1 [Liu, Z. H.; Zhong, S.; Friesen, T. L.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. [Stasko, A. K.; Edwards, M. C.; Friesen, T. L.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. RP Friesen, TL (reprint author), ARS, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. EM timothy.friesen@ars.usda.gov FU USDA-ARS CRIS [5442-22000-043-00D]; USDA-NIFA-AFRI [2011-68002-30029]; American Malting Barley Association FX This research was supported by USDA-ARS CRIS Project 5442-22000-043-00D, USDA-NIFA-AFRI Triticeae Coordinated Agricultural Project (T-CAP) Competitive Grant 2011-68002-30029 and The American Malting Barley Association. We thank D. Holmes for technical support. NR 53 TC 12 Z9 12 U1 0 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2012 VL 102 IS 5 BP 539 EP 546 DI 10.1094/PHYTO-09-11-0243 PG 8 WC Plant Sciences SC Plant Sciences GA 927ZW UT WOS:000302950300010 PM 22494251 ER PT J AU Quito-Avila, DF Lightle, D Lee, J Martin, RR AF Quito-Avila, Diego F. Lightle, Danielle Lee, Jana Martin, Robert R. TI Transmission Biology of Raspberry latent virus, the First Aphid-Borne Reovirus SO PHYTOPATHOLOGY LA English DT Article ID WHITEFLY BEMISIA-TABACI; PLANT-VIRUSES; CIRCULATIVE TRANSMISSION; ARTHROPOD TRANSMISSION; ENDOSYMBIOTIC BACTERIA; VECTOR INTERACTIONS; MYZUS-PERSICAE; MOTTLE-VIRUS; RT-PCR; IDENTIFICATION AB Raspberry latent virus (RpLV) is a newly characterized reovirus found in commercial raspberry fields in the Pacific Northwest (PNW). Thus far, all members of the plant reoviruses are transmitted in a replicative, persistent manner by several species of leafhoppers or planthoppers. After several failed attempts to transmit RpLV using leafhoppers, the large raspberry aphid, commonly found in the PNW, was tested as a vector of the virus. The virus was transmitted to new, healthy raspberry plants when inoculated with groups of at least 50 viruliferous aphids, suggesting that aphids are vectors of RpLV, albeit inefficient ones. Using absolute and relative quantification methods, it was shown that the virus titer in aphids continued to increase after the acquisition period even when aphids were serially transferred onto fresh, healthy plants on a daily basis. Transmission experiments determined that RpLV has a 6-day latent period in the aphid before it becomes transmissible; however, it was not transmitted transovarially to the next generation. To our knowledge, this is the first report of a plant reovirus transmitted by an aphid. Phylogenetic analyses showed that RpLV is related most closely to but distinct from Rice ragged stunt virus (RRSV), the type member of the genus Oryzavirus. Moreover, the conserved nucleotide termini of the genomic segments of RpLV did not match those of RRSV or other plant reoviruses, allowing us to suggest that RpLV is probably the type member of a new genus in the Reoviridae comprising aphid-transmitted reoviruses. C1 [Quito-Avila, Diego F.; Martin, Robert R.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Lightle, Danielle; Lee, Jana] Oregon State Univ, Entomol Program, Corvallis, OR 97331 USA. [Lee, Jana; Martin, Robert R.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR 97331 USA. RP Quito-Avila, DF (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM quitod@onid.orst.edu FU United States Department of Agriculture under the Specialty Crops Research Initiative (SCRI) [2009-51181-06022, CRIS 5358-22000-032-00D] FX This work was partially funded by the United States Department of Agriculture under the Specialty Crops Research Initiative (SCRI), award number 2009-51181-06022, CRIS 5358-22000-032-00D. We thank N. Mosier, North American Plants Inc., and Sakuma Brothers Farm for donating plant material; I. Tzanetakis for proofreading the article; and S. Sabanadzovic and D. Stenger for sharing unpublished data on new reoviruses. NR 48 TC 11 Z9 11 U1 2 U2 11 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD MAY PY 2012 VL 102 IS 5 BP 547 EP 553 DI 10.1094/PHYTO-12-11-0331 PG 7 WC Plant Sciences SC Plant Sciences GA 927ZW UT WOS:000302950300011 PM 22352304 ER PT J AU Campbell, TA Long, DB Lavelle, MJ Leland, BR Blankenship, TL VerCauteren, KC AF Campbell, Tyler A. Long, David B. Lavelle, Michael J. Leland, Bruce R. Blankenship, Terry L. VerCauteren, Kurt C. TI Impact of baiting on feral swine behavior in the presence of culling activities SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Baiting; Culling; Depopulation; Epidemic; Eradication; Feral swine; Sus scrofa ID PIGS SUS SCROFA; HOME-RANGE; SOUTHERN TEXAS; MOUTH-DISEASE; WILD; POPULATIONS; CALIFORNIA; PHARMACEUTICALS; ERADICATION; MANAGEMENT AB We studied the effects of baiting on feral swine (Sus scrofa) movements and corresponding likelihood of disease spread under real and simulated culling pressure. Our objectives were to determine the proportion of feral swine that used the bait station site, and if baiting of feral swine altered areas of utilization, distances from location centroids to treatment location (control or bait station), and movement rates by survivors during culling activities. We hypothesized that the bait station would increase the sedentary nature of feral swine, thus reducing the potential for dispersal and hence disease dispersal. Our experiment was conducted between February and May 2011 on the Rob and Bessie Welder Wildlife Foundation (WWF) in San Patricio County, Texas. We trapped 83 feral swine and placed GPS collars on 21 animals. We established and maintained a centralized bait station on one side of the WWF from 13 March to 27 April. We conducted population-wide culling activities, including trapping, controlled shooting, drive shooting, and aerial gunning, from 3 to 27 April and removed 143 feral swine (4.6 feral swine/km(2)). Areas of utilization did not differ between treatments (control or bait station). However, we found location centroids of bait station site feral swine to be closer to the treatment location than those of control site animals and daily movement rates of bait station site feral swine to be 39% greater than movement rates of control site animals. Based on our observation that only 62% of feral swine trapped in proximity to the bait station used it, we cannot recommend baiting as an alternative to fences for containing animals during culling activities. However, there is value in using bait stations to describe patterns of feral swine movements, facilitate observation, and improve efficacy when conducting removals. Published by Elsevier B.V. C1 [Campbell, Tyler A.; Long, David B.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Florida Field Stn, Gainesville, FL 32641 USA. [Lavelle, Michael J.; VerCauteren, Kurt C.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Leland, Bruce R.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, San Antonio, TX 78249 USA. [Blankenship, Terry L.] Welder Wildlife Fdn, Sinton, TX 78387 USA. RP Campbell, TA (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Florida Field Stn, 2820 E Univ Ave, Gainesville, FL 32641 USA. EM tyler.a.campbell@aphis.usda.gov; david.b.long@aphis.usda.gov; michael.j.lavelle@usda.aphis.gov; bruce.r.leland@aphis.usda.gov; tblankenship@welderwildlife.org; kurt.c.vercauteren@usda.aphis.gov FU United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center FX The study was funded by United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center. We thank Welder Wildlife Foundation for property access. We thank E. Davis, S. Rabe, and J. Rattan for field assistance. Logistical support was provided by the Caesar Kleberg Wildlife Research Institute at Texas A&M University-Kingsville and Texas Wildlife Services. The USDA does not recommend endorsement, guarantee nor warrant companies or commercial products mentioned in this study. NR 53 TC 1 Z9 1 U1 1 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 J9 PREV VET MED JI Prev. Vet. Med. PD MAY 1 PY 2012 VL 104 IS 3-4 BP 249 EP 257 DI 10.1016/j.prevetmed.2012.01.001 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 929AF UT WOS:000303032900007 PM 22305877 ER PT J AU Harvey, BC Railsback, SF AF Harvey, B. C. Railsback, S. F. TI EFFECTS OF PASSAGE BARRIERS ON DEMOGRAPHICS AND STABILITY PROPERTIES OF A VIRTUAL TROUT POPULATION SO RIVER RESEARCH AND APPLICATIONS LA English DT Article DE barriers; habitat fragmentation; individual-based modelling; persistence; resistance; trout; upstream fish passage ID MOVEMENT; LANDSCAPES; SALMONIDS; PROTOCOL; MODELS; RULES; FISH AB Habitat fragmentation is widely assumed to have negative effects on populations and communities, but some effects of fragmentation are subtle, difficult to measure and not always negative. For stream fish, barriers to upstream passage, such as waterfalls or culverts with perched outlets, are a common cause of fragmentation. We explored the effects of barriers on a virtual stream trout population occupying a network of reaches in the inSTREAM individual-based trout population model. The model includes daily habitat selection by individual fish, with barriers represented by assuming trout cannot move upstream past a barrier and move downstream over a barrier only if habitat above it offers low potential fitness. In 78-year simulations of a resident trout population occupying the stream network of a 25-km2 catchment, we varied barrier density and observed effects on population stability properties and demographics. Increasing barrier density decreased subpopulation persistence in first-order tributaries but not larger streams. Barriers at tributary mouths reduced the population's resistance to extreme flows, but additional barriers caused no further loss of resistance. Barriers reduced overall abundance and biomass at intermediate and high densities and caused a small but surprising increase in biomass at low density. In comparison with fish in the remainder of the network, fish in isolated first-order tributaries had higher survival early in life, smaller sizes at age and reproduced as smaller, younger individuals. Fish that passed over barriers contributed relatively little to downstream populations but, had they not moved, would have increased persistence and abundance of the tributaries they moved from. Relationships such as these would be very difficult to elucidate without a model representing individual behaviour. Copyright (C) 2011 John Wiley & Sons, Ltd. C1 [Railsback, S. F.] Railsback & Associates, Lang, Arcata, CA 95521 USA. [Harvey, B. C.] US Forest Serv, USDA, Pacific SW Res Stn, Arcata, CA USA. RP Railsback, SF (reprint author), Railsback & Associates, Lang, 250 Calif Ave, Arcata, CA 95521 USA. EM Steve@LangRailsback.com OI Railsback, Steven/0000-0002-5923-9847 NR 17 TC 4 Z9 4 U1 0 U2 18 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1535-1459 J9 RIVER RES APPL JI River Res. Appl. PD MAY PY 2012 VL 28 IS 4 SI SI BP 479 EP 489 DI 10.1002/rra.1574 PG 11 WC Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA 927UY UT WOS:000302937000007 ER PT J AU Templer, PH Schiller, AF Fuller, NW Socci, AM Campbell, JL Drake, JE Kunz, TH AF Templer, Pamela H. Schiller, Andrew F. Fuller, Nathan W. Socci, Anne M. Campbell, John L. Drake, John E. Kunz, Thomas H. TI Impact of a reduced winter snowpack on litter arthropod abundance and diversity in a northern hardwood forest ecosystem SO BIOLOGY AND FERTILITY OF SOILS LA English DT Article DE Climate change; Hardwood forest; Hubbard Brook experimental forest; Northern forest; Litter arthropods; Soil fauna ID CLIMATE-CHANGE PROJECTIONS; INSECT COLD-HARDINESS; GROUND FOOD WEBS; LOW-TEMPERATURE; NITROGEN MINERALIZATION; TROPHIC STRUCTURE; FUTURE CHANGES; SOIL ANIMALS; DECOMPOSITION; SENSITIVITY AB Projected changes in climate for the northeastern USA over the next 100 years include a reduction in the depth and duration of the winter snowpack, which could affect soil temperatures and frost regimes. We conducted a snow-removal experiment in a northern hardwood forest at the Hubbard Brook Experimental Forest in central New Hampshire over 2 years to induce soil freezing and evaluate its effect on the abundance, richness, and diversity of soil arthropods during the growing season. Snow removal at the beginning of winter increased the depth and duration of soil frost, decreased soil temperatures, and led to a reduced abundance of some arthropod taxa, including Araneae (reduced by 57%; P = 0.0001), Pseudoscorpionida (75%; P < 0.0001), Hymenoptera (57%; P = 0.0033), Collembola (24%; P = 0.0019), adult Coleoptera (23%; P = 0.0057), and larval Diptera (33%; P < 0.0001) and an increase in other taxa, including Hemiptera (increased by 7%; P = 0.032). Taxa that did not respond significantly to snow removal included Chilopoda (P = 0.55), Acari (P = 0.66), Diplopoda (P = 0.66), adult Diptera (P = 0.54), and larval Coleoptera (P = 0.39). A delayed snowpack over two winters also resulted in decreased arthropod richness by 30% (P < 0.0001) and Simpson's index of diversity by 22% (P = 0.0002) during the two subsequent growing seasons. Results of this study demonstrate that predicted changes in the winter snowpack and depth and duration of soil frost may reduce the abundance and alter the community composition of arthropods living in the forest floor of northern hardwood forests, which could have implications for the structure and function of northern forest ecosystems. C1 [Templer, Pamela H.; Schiller, Andrew F.; Fuller, Nathan W.; Socci, Anne M.; Drake, John E.; Kunz, Thomas H.] Boston Univ, Ctr Ecol & Conservat Biol, Dept Biol, Boston, MA 02215 USA. [Campbell, John L.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. RP Templer, PH (reprint author), Boston Univ, Ctr Ecol & Conservat Biol, Dept Biol, 5 Cummington St, Boston, MA 02215 USA. EM ptempler@bu.edu RI Drake, John/N-8490-2014; OI Drake, John/0000-0003-4274-4780; Campbell, John/0000-0003-4956-1696 FU Andrew W. Mellon Foundation; Boston University; Northeastern States Research Cooperative (NSRC); University of Vermont; University of New Hampshire; University of Maine; SUNY-ESF; Northern Research Station, USDA Forest Service; National Science Foundation FX Funding for this project was provided by the Andrew W. Mellon Foundation, the Boston University Undergraduate Research Opportunities Program (UROP), and the Northeastern States Research Cooperative (NSRC) - a joint program of the University of Vermont, the University of New Hampshire, the University of Maine, SUNY-ESF, and the Northern Research Station, USDA Forest Service. We thank Duncan Adams, Justin Brigham, Christopher Freeman, Meghan Gagne, Glenn Harrington, Stephanie Juice, Michael Mangiante, Matt Ross, Bethel Steele, Phil Thompson, Alexandra Webster, and Tammy Wooster for field assistance. Ian Halm from the USDA Forest Service provided logistical support at the Hubbard Brook Experimental Forest. We are grateful for helpful comments and suggestions on an earlier draft of this manuscript from Stephanie Juice, Nick Rodenhouse, Kimberly Wallin and Alexandra Webster. This manuscript is a contribution of the Hubbard Brook Ecosystem Study. Hubbard Brook is part of the Long-Term Ecological Research (LTER) network, which is supported by the National Science Foundation. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, PA. NR 55 TC 13 Z9 14 U1 16 U2 80 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0178-2762 J9 BIOL FERT SOILS JI Biol. Fertil. Soils PD MAY PY 2012 VL 48 IS 4 BP 413 EP 424 DI 10.1007/s00374-011-0636-3 PG 12 WC Soil Science SC Agriculture GA 926CU UT WOS:000302809700006 ER PT J AU Kneeland, K Coudron, TA Lindroth, E Stanley, D Foster, JE AF Kneeland, Kathleen Coudron, Thomas A. Lindroth, Erica Stanley, David Foster, John E. TI Genetic variation in field and laboratory populations of the spined soldier bug, Podisus maculiventris SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE AFLP; predatory bug; Pentatomidae; genetic similarity; Heteroptera; stink bug ID SAY HETEROPTERA; COLEOPTERA-CHRYSOMELIDAE; HEMIPTERA-PENTATOMIDAE; ARTIFICIAL DIET; PREDATION; DIPTERA; BEETLE; COST; AFLP AB The predatory spined soldier bug, Podisus maculiventris (Say) (Heteroptera: Pentatomidae), is an economically important and highly valued biological control agent. There is substantial information on the biology, ecology, behavior, and rearing of this stink bug. However, virtually nothing is known of its genetic variation, in natural or domesticated populations. To address this lacuna, we used amplified fragment length polymorphism (AFLP) to assess the genetic variability of field and laboratory populations. Four AFLP universal primer combinations yielded a total of 209 usable loci. The AFLP results showed greater genetic variability between populations from Missouri and Mississippi (both USA), and relatively low variability within Missouri populations. We infer little genetic isolation among Missouri field populations and within laboratory populations, but a significant genetic isolation between Missouri and Mississippi populations. C1 [Coudron, Thomas A.; Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Kneeland, Kathleen; Lindroth, Erica; Foster, John E.] Univ Nebraska, Dept Entomol, Insect Genet Lab, Lincoln, NE 68583 USA. RP Coudron, TA (reprint author), USDA ARS, Biol Control Insects Res Lab, 1503 S Providence Rd, Columbia, MO 65203 USA. EM tom.coudron@ars.usda.gov NR 32 TC 4 Z9 5 U1 1 U2 13 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD MAY PY 2012 VL 143 IS 2 BP 120 EP 126 DI 10.1111/j.1570-7458.2012.01239.x PG 7 WC Entomology SC Entomology GA 923GU UT WOS:000302608500003 ER PT J AU Lopes, AP Dubey, JP Moutinho, O Gargate, MJ Vilares, A Rodrigues, M Cardoso, L AF Lopes, A. P. Dubey, J. P. Moutinho, O. Gargate, M. J. Vilares, A. Rodrigues, M. Cardoso, L. TI Seroepidemiology of Toxoplasma gondii infection in women from the North of Portugal in their childbearing years SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Epidemiology; public health; Toxoplasma gondii; toxoplasmosis; zoonoses ID NORTHEASTERN PORTUGAL; RISK-FACTORS; PREGNANCY; OOCYST AB Seroprevalence of Toxoplasma gondii infection and associated risk factors were investigated in 401 women of childbearing age from the North of Portugal. Of the 98 (24.4%) seropositive women, 92 (93.9%) only had immunoglobulin (Ig) G, two (2.0%) only had IgM, and four (4.1%) others had both IgG and IgM. Risk factors for T. gondii infection in women were: engaging in soil-related activities without gloves [odds ratio (OR) 8.4], consumption of unwashed raw vegetables or fruit (OR 7.6), and consumption of smoked or cured (non-cooked) processed pork products (OR 2.5). Most women of childbearing age from the North Portugal are susceptible to primary infection with T. gondii and, therefore, the risk of congenital toxoplasmosis remains high. C1 [Lopes, A. P.; Rodrigues, M.; Cardoso, L.] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, P-5001801 Vila Real, Portugal. [Lopes, A. P.; Rodrigues, M.; Cardoso, L.] Univ Tras os Montes & Alto Douro, Ctr Vet & Anim Sci, P-5001801 Vila Real, Portugal. [Dubey, J. P.] ARS, Anim Parasit Dis Lab, Anim Nat Resources Inst, USDA, Beltsville, MD USA. [Moutinho, O.] Hosp Ctr Tras Os Montes & Alto Douro, Serv Gynaecol & Obstet, Vila Real, Portugal. [Gargate, M. J.; Vilares, A.] Natl Inst Hlth, Dept Infect Dis, Lisbon, Portugal. [Cardoso, L.] Univ Porto, Inst Biol Mol & Celular, Parasite Dis Grp, Oporto, Portugal. RP Lopes, AP (reprint author), Univ Tras Os Montes & Alto Douro, Dept Vet Sci, POB 1013, P-5001801 Vila Real, Portugal. EM aplopes@utad.pt RI Cardoso, Luis/J-8310-2013; OI Cardoso, Luis/0000-0002-6145-7560; Lopes, Ana Patricia/0000-0002-8182-5674 FU Fundacao para a Ciencia e a Tecnologia, Portugal [SFRH/BD/44438/2008]; European Social Fund FX The authors thank the women who responded to the questionnaire for their cooperation. Special thanks are also due to Nurse Manuela P. Silva and Ms. Julia Torres for their assistance. A. P. Lopes was supported by scholarship SFRH/BD/44438/2008 from Fundacao para a Ciencia e a Tecnologia, Portugal, and the European Social Fund. NR 27 TC 20 Z9 20 U1 0 U2 3 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD MAY PY 2012 VL 140 IS 5 BP 872 EP 877 DI 10.1017/S0950268811001658 PG 6 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA 919ZV UT WOS:000302371600014 PM 21878147 ER PT J AU Srinivas, G Huang, YH Carver, BF Mornhinweg, D AF Srinivas, G. Huang, Yinghua Carver, Brett F. Mornhinweg, Do TI AFLP genetic diversity analysis in Russian wheat aphid resistant wheat accessions SO EUPHYTICA LA English DT Article DE Wheat; Resistance; Biotypes; Amplified fragment length polymorphism; Genetic diversity ID WINTER-WHEAT; BREAD WHEAT; HOMOPTERA; INHERITANCE; CULTIVARS; MARKERS; POPULATIONS; COLLECTION; GERMPLASM; PI294994 AB Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is an important insect pest which causes severe economic losses in wheat (Triticum spp.). Among the various U.S. RWA biotypes, biotype 1 (RWA1) and biotype 2 (RWA2) are the most prevalent and most virulent on cultivated genotypes. Although many sources of resistance to these biotypes are available among landraces, their relatedness should be characterized to permit their more efficient use in breeding programs. In this study, 38 hexaploid accessions resistant to biotype 1 and/or biotype 2 were evaluated for genetic diversity based on amplified fragment length polymorphisms (AFLP). Fifteen AFLP selective primer combinations were used to genotype these accessions, resulting in 893 amplicons. Of these, 274 (30.6%) informative polymorphic bands were used for genetic diversity analysis. Genetic similarity coefficients ranged from 0.47 to 0.87 among the resistant accessions, indicating high genetic diversity among them. Cluster analysis grouped the 38 accessions into two major clusters, I and II, including resistant lines for RWA1 and RWA2. The study indicated that accessions in the National Small Grains Collection conferring RWA1 or RWA2 resistance comprise a diversified population which should support introgression efforts and provide genetic diversity for future breeding for RWA resistance. C1 [Srinivas, G.; Huang, Yinghua; Mornhinweg, Do] ARS, USDA, Plant Sci Res Lab, Stillwater, OK 74075 USA. [Srinivas, G.; Carver, Brett F.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. RP Huang, YH (reprint author), ARS, USDA, Plant Sci Res Lab, 1301 N Western Rd, Stillwater, OK 74075 USA. EM yinghua.huang@ars.usda.gov NR 39 TC 0 Z9 0 U1 2 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD MAY PY 2012 VL 185 IS 1 BP 27 EP 35 DI 10.1007/s10681-011-0605-9 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 919ZE UT WOS:000302369900003 ER PT J AU Li, T Bai, GH Wu, SY Gu, SL AF Li, Tao Bai, Guihua Wu, Shuangye Gu, Shiliang TI Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu SO EUPHYTICA LA English DT Article DE Head scab; Wheat landrace; Fusarium head blight resistance; Quantitative trait loci; Genetic effects ID LENGTH POLYMORPHISM MARKERS; SPRING WHEAT; QTL ANALYSIS; MOLECULAR CHARACTERIZATION; SCAB RESISTANCE; FUNGAL SPREAD; II RESISTANCE; MAJOR QTL; POPULATION; WANGSHUIBAI AB The Chinese wheat landrace Huangfangzhu (HFZ) has a high level of resistance to Fusarium head blight (FHB). To identify chromosomal regions that are responsible for FHB resistance in HFZ, F-8 recombinant inbred lines (RIL) were developed from a cross between HFZ and Wheaton, a U.S. hard spring wheat. FHB was evaluated by single floret inoculation in both greenhouse and field environments. Two quantitative trait loci (QTL) with major effects were identified. One QTL was located on the short arm of chromosome 3B, and explained 35.4% of the phenotypic variation; the other QTL was assigned to 7AL and explained 18.0% of the phenotypic variation for FHB response. In addition, three minor QTL were detected on chromosomes 1AS, 1B and 5AS by single marker regression. HFZ contributed all favorable alleles. The RIL with HFZ alleles at the QTL on 3BS and 7AL displayed significantly lower percentages of infected spikelets than RIL without these alleles in both greenhouse and field environments. HFZ combined several alleles from germplasm reported previously and is a promising alternative source for improving wheat FHB resistance. C1 [Li, Tao; Gu, Shiliang] Yangzhou Univ, Minist Educ, Key Lab Plant Funct Genom, Jiangsu Prov Key Lab Crop Genet & Physiol, Yangzhou 225009, Peoples R China. [Li, Tao] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Bai, Guihua; Wu, Shuangye] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Bai, Guihua] ARS, USDA, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Li, T (reprint author), Yangzhou Univ, Minist Educ, Key Lab Plant Funct Genom, Jiangsu Prov Key Lab Crop Genet & Physiol, Yangzhou 225009, Peoples R China. EM taoli@yzu.edu.cn; guihua.bai@ars.usda.gov FU NSFC [31171537]; Jiangsu Higher Education Institution; Jiangsu Provincial Natural Science Foundation of China [BK2010312]; National Research Initiative from the USDA National Institute of Food and Agriculture [2011-68002-30029] FX This project is partly funded by NSFC (Grant no. 31171537), the Priority Academic Program Development of Jiangsu Higher Education Institution, Jiangsu Provincial Natural Science Foundation of China (Grant no. BK2010312), and National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This is contribution no. 11-207-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas, USA. NR 37 TC 6 Z9 9 U1 3 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD MAY PY 2012 VL 185 IS 1 BP 93 EP 102 DI 10.1007/s10681-012-0631-2 PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 919ZE UT WOS:000302369900008 ER PT J AU van Nocker, S Berry, G Najdowski, J Michelutti, R Luffman, M Forsline, P Alsmairat, N Beaudry, R Nair, MG Ordidge, M AF van Nocker, Steven Berry, Garrett Najdowski, James Michelutti, Roberto Luffman, Margie Forsline, Philip Alsmairat, Nihad Beaudry, Randy Nair, Muraleedharan G. Ordidge, Matthew TI Genetic diversity of red-fleshed apples (Malus) SO EUPHYTICA LA English DT Article DE Malus; Anthocyanin; Genetic diversity; Fruit; Apple; Crab; MdMYB10; MYB10 ID TRANSCRIPTION FACTOR; SKIN AB Anthocyanins are flavonoid pigments imparting red, blue, or purple pigmentation to fruits, flowers and foliage. These compounds are powerful antioxidants in vitro, and are widely believed to contribute to human health. The fruit of the domestic apple (Malus x domestica) is a popular and important source of nutrients, and is considered one of the top 'functional foods'aEuro"those foods that have inherent health-promoting benefits beyond basic nutritional value. The pigmentation of typical red apple fruits results from accumulation of anthocyanin in the skin. However, numerous genotypes of Malus are known that synthesize anthocyanin in additional fruit tissues including the core and cortex (flesh). Red-fleshed apple genotypes are an attractive starting point for development of novel varieties for consumption and nutraceutical use through traditional breeding and biotechnology. However, cultivar development is limited by lack of characterization of the diversity of genetic backgrounds showing this trait. We identified and cataloged red-fleshed apple genotypes from four Malus diversity collections representing over 3,000 accessions including domestic cultivars, wild species, and named hybrids. We found a striking range of flesh color intensity and pattern among accessions, including those carrying the MYB10 R (6) allele conferring ectopic expression of a key transcriptional regulator of anthocyanin biosynthesis. Although MYB10 R (6) was strongly associated with red-fleshed fruit among genotypes, this allele was neither sufficient nor required for this trait in all genotypes. Nearly all red-fleshed accessions tested could be traced back to 'Niedzwetzkyana', a presumed natural form of M. sieversii native to central Asia. C1 [van Nocker, Steven; Berry, Garrett; Najdowski, James; Alsmairat, Nihad; Beaudry, Randy; Nair, Muraleedharan G.] Michigan State Univ, Dept Hort, Pomol Grp, E Lansing, MI 48824 USA. [Michelutti, Roberto; Luffman, Margie] Agr & AgriFood Canada, Harrow, ON, Canada. [Forsline, Philip] USDA ARS, Geneva, NY USA. RP van Nocker, S (reprint author), Michigan State Univ, Dept Hort, Pomol Grp, E Lansing, MI 48824 USA. EM vannocke@msu.edu FU Michigan Apple Commission; Michigan Department of Agriculture [791N0200091]; Education Foundation of the American Society of Plant Biologists FX We thank Jeff Wilson (MSU Campus Planning and Administration) and Dr. Frank Telewski (MSU Department of Plant Biology and MSU Campus Planning and Administration) for assistance with locating Malus accessions within the MSU campus. We acknowledge the assistance of Tyler Harris, MSU Multicultural Apprentice Program, with fruit collection and processing and Mary Pennell (NFC Horticultural Curator). This work was supported by the Michigan Apple Commission, the Michigan Department of Agriculture (Grant No. 791N0200091), and the Education Foundation of the American Society of Plant Biologists. NR 18 TC 10 Z9 10 U1 4 U2 47 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD MAY PY 2012 VL 185 IS 2 BP 281 EP 293 DI 10.1007/s10681-011-0579-7 PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 923XK UT WOS:000302653800011 ER PT J AU Calfee, MW Ryan, SP Wood, JP Mickelsen, L Kempter, C Miller, L Colby, M Touati, A Clayton, M Griffin-Gatchalian, N McDonald, S Delafield, R AF Calfee, M. W. Ryan, S. P. Wood, J. P. Mickelsen, L. Kempter, C. Miller, L. Colby, M. Touati, A. Clayton, M. Griffin-Gatchalian, N. McDonald, S. Delafield, R. TI Laboratory evaluation of large-scale decontamination approaches SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Anthrax; Bacillus anthracis; biological agent; decontamination; facility remediation; foreign animal disease; spore; sporicide; wide-area incident ID BACILLUS-ANTHRACIS SPORES; NONPOROUS SURFACES; QUANTITATIVE PCR; INACTIVATION; DISINFECTANTS; HYPOCHLORITE; STRATEGIES; COLLECTION; RESISTANCE; EFFICACY AB Aims: To evaluate the effectiveness of two spray-based decontamination methods for surface contamination reduction and to determine the potential for contamination spread by these methods. Methods and Results: Material coupons (treated plywood and concrete) were contaminated with c. 1 x 10(7) spores of Bacillus atrophaeus by aerosol deposition. Decontaminants (pH-adjusted bleach or Spor-Klenz (R) RTU) were applied to coupons by either backpack sprayer or gas-powered sprayer. Contact time, reapplication frequency and rinse method were also varied. In addition to surface removal efficacy, partitioning of contamination between the rinsate and aerosol fractions was determined. Results indicated that pH-adjusted bleach was effective (6 logs reduction) when two applications and a 30 min contact time were administered, regardless of the decontaminant application method or material. Spor-Klenz (R) RTU was effective on wood, but achieved <= 3 logs reduction on concrete. A shortened application procedure with pH-adjusted bleach resulted in lower efficacy on wood, and a greater apparent potential for contamination spread. Conclusions: Consideration of material surface type is important when selecting a decontaminant. Also, achieving conditions that effectively inactivate surface biological contamination are critical to preventing the spread of contamination. Significance and Impact of the Study: Results presented here are intended to help development of remediation plans following a biological contamination incident. C1 [Calfee, M. W.; Ryan, S. P.; Wood, J. P.] US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, Res Triangle Pk, NC 27711 USA. [Mickelsen, L.] US EPA, Off Emergency Management, Natl Decontaminat Team, Res Triangle Pk, NC 27711 USA. [Kempter, C.] US EPA, Off Chem Safety & Pollut Prevent, Arlington, VA USA. [Miller, L.] US Anim & Plant Hlth Inspect Serv, USDA, Riverdale, MD USA. [Colby, M.] US Dept Homeland Secur, Sci & Technol Directorate, Washington, DC USA. [Touati, A.; Clayton, M.; Griffin-Gatchalian, N.; McDonald, S.; Delafield, R.] Arcadis Geraghty & Miller Inc, Durham, NC USA. RP Calfee, MW (reprint author), US EPA, Off Res & Dev, Natl Homeland Secur Res Ctr, MD E343-06,109 TW Alexander Dr, Res Triangle Pk, NC 27711 USA. EM calfee.worth@epa.gov OI Wood, Joseph/0000-0001-6316-9418 FU Interagency with the Science and Technology Directorate of the US Department of Homeland Security [HSHQDC-10-X-00148] FX The US Environmental Protection Agency through its Office of Research and Development directed the research described herein under EP-C-09-027 with ARCADIS Inc. This project was funded through an Interagency with the Science and Technology Directorate of the US Department of Homeland Security under Award Number HSHQDC-10-X-00148. This manuscript has been subject to an administrative review but does not necessarily reflect the views of the Agency. No official endorsement should be inferred. EPA does not endorse the purchase or sale of any commercial products or services. NR 26 TC 9 Z9 9 U1 1 U2 17 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAY PY 2012 VL 112 IS 5 BP 874 EP 882 DI 10.1111/j.1365-2672.2012.05259.x PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 923IJ UT WOS:000302612700004 PM 22332972 ER PT J AU Crippen, TL Zheng, L Sheffield, CL Tomberlin, JK Beier, RC Yu, Z AF Crippen, T. L. Zheng, L. Sheffield, C. L. Tomberlin, J. K. Beier, R. C. Yu, Z. TI Transient gut retention and persistence of Salmonella through metamorphosis in the lesser mealworm, Alphitobius diaperinus (Coleoptera: Tenebrionidae) SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE Alphitobius diaperinus; bacteria; insect metamorphosis; lesser mealworm; retention; Salmonella ID GREEN FLUORESCENT PROTEIN; ENTERITIDIS INFECTION; DEVELOPMENTAL-STAGES; PATHOGENIC BACTERIA; LARVAL DEVELOPMENT; DIPTERA; MUSCIDAE; CALLIPHORIDAE; CHICKENS; FLIES AB Aims: This study was undertaken to determine the retention of Salmonella through Alphitobius diaperinus metamorphosis and its contribution, through defecation, to external contamination. Methods and Results: Insects were exposed to a tagged Salmonella enterica and evaluated for external elimination. (i) Each day for 3 weeks, a filter collected frass from a restrained insect for analysis. (ii) Exposed larvae in a closed container were followed through pupation, and newly emerged adults were examined for their retention of marker bacteria. Conclusions: Exposed adults and larvae produced Salmonella-positive frass for an average of 8 days, ranging from 6 to 11 days and 6 to 12 days, respectively. Nineteen per cent of the larvae carried Salmonella through metamorphosis and eclosion, with 5% of the pupal exuviae being positive as well. Significance and Impact of the Study: Many sources of foodborne pathogens within the poultry production facilities, including reservoir populations, currently go unrecognized. This diminishes the ability of producers to mitigate the transfer of pathogens between animals, humans and the environment. Poultry management standards accept the reutilization of litter. Alphitobius diaperinus survive between flock rotations on the reutilized litter, and it was demonstrated in this study that the Salmonella they carry can survive with them. C1 [Crippen, T. L.; Sheffield, C. L.; Beier, R. C.] ARS, Food & Feed Safety Res Unit, SPARC, USDA, College Stn, TX 77845 USA. [Zheng, L.; Yu, Z.] Huazhong Agr Univ, Natl Engn Res Ctr Microbial Pesticides, State Key Lab Agr Microbiol, Wuhan, Peoples R China. [Zheng, L.; Tomberlin, J. K.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. RP Crippen, TL (reprint author), ARS, Food & Feed Safety Res Unit, SPARC, USDA, 2881 F&B Rd, College Stn, TX 77845 USA. EM tc.crippen@ars.usda.gov NR 31 TC 6 Z9 6 U1 1 U2 19 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD MAY PY 2012 VL 112 IS 5 BP 920 EP 926 DI 10.1111/j.1365-2672.2012.05265.x PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 923IJ UT WOS:000302612700009 PM 22380581 ER PT J AU Langman, VA Rowe, MF Roberts, TJ Langman, NV Taylor, CR AF Langman, Vaughan A. Rowe, Michael F. Roberts, Thomas J. Langman, Nathanial V. Taylor, Charles R. TI Minimum cost of transport in Asian elephants: do we really need a bigger elephant? SO JOURNAL OF EXPERIMENTAL BIOLOGY LA English DT Article DE Asian elephant; locomotion; cost of transport; allometry ID LOXODONTA-AFRICANA; LOCOMOTION; SPEED; GAIT; TRANSITION; ENERGETICS; MECHANICS; MOVEMENTS; MAMMALS; WALKING AB Body mass is the primary determinant of an animal's energy requirements. At their optimum walking speed, large animals have lower mass-specific energy requirements for locomotion than small ones. In animals ranging in size from 0.8 g (roach) to 260 kg (zebu steer), the minimum cost of transport (COTmin) decreases with increasing body size roughly as COTmin proportional to body mass (M-b)(-0.316+/-0.023) (95% CI). Typically, the variation of COTmin with body mass is weaker at the intraspecific level as a result of physiological and geometric similarity within closely related species. The interspecific relationship estimates that an adult elephant, with twice the body mass of a mid-sized elephant, should be able to move its body approximately 23% cheaper than the smaller elephant. We sought to determine whether adult Asian and sub-adult African elephants follow a single quasi-intraspecific relationship, and extend the interspecific relationship between COTmin and body mass to 12-fold larger animals. Physiological and possibly geometric similarity between adult Asian elephants and sub-adult African elephants caused body mass to have a no effect on COTmin (COTmin proportional to M-b(0.007+/-0.455)). The COTmin in elephants occurred at walking speeds between 1.3 and similar to 1.5 ms(-1), and at Froude numbers between 0.10 and 0.24. The addition of adult Asian elephants to the interspecific relationship resulted in COTmin proportional to M-b(-0.277+/-0.046). The quasi-intraspecific relationship between body mass and COTmin among elephants caused the interspecific relationship to underestimate COTmin in larger elephants. C1 [Langman, Vaughan A.] USDA APHIS, Bellvue, CO 80512 USA. [Rowe, Michael F.] Indiana State Univ, Dept Biol, Terre Haute, IN 47802 USA. [Roberts, Thomas J.] Brown Univ, Providence, RI 02912 USA. [Langman, Nathanial V.] Dartmouth Coll, Thayer Sch Engn, Hanover, NH 03755 USA. [Taylor, Charles R.] Harvard Univ, Concord Field Stn, Bedford, MA 01730 USA. RP Langman, VA (reprint author), USDA APHIS, 816 White Pine Dr, Bellvue, CO 80512 USA. EM vaughan.a.langman@aphis.usda.gov NR 43 TC 12 Z9 12 U1 1 U2 22 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0022-0949 J9 J EXP BIOL JI J. Exp. Biol. PD MAY PY 2012 VL 215 IS 9 BP 1509 EP 1514 DI 10.1242/jeb.063032 PG 6 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 925UE UT WOS:000302786300019 PM 22496287 ER PT J AU Sengoda, VG Tsai, WS de la Pena, RC Green, SK Kenyon, L Hughes, J AF Sengoda, Venkatesan G. Tsai, Wen-Shi de la Pena, Robert C. Green, Sylvia K. Kenyon, Lawrence Hughes, Jackie TI Expression of the Full-length Coat Protein Gene of Tomato leaf curl Taiwan virus is Not Necessary for Recovery Phenotype in Transgenic Tomato SO JOURNAL OF PHYTOPATHOLOGY LA English DT Article DE coat-protein gene; Tomato leaf curl Taiwan virus; transgenic tomato ID TOBACCO-MOSAIC-VIRUS; CAPSID PROTEIN; WHITEFLY TRANSMISSION; MEDIATED RESISTANCE; GEMINIVIRUS; PLANTS; INFECTION; MOVEMENT; TYLCV; HOST AB Transgenic tomato plants expressing full-length (CPV1) and truncated coat protein (CP) gene (CPV2) of Tomato leaf curl Taiwan virus (ToLCTWV) were generated by Agrobacterium-mediated transformation. Transgene integration and expression was confirmed by PCR and Southern blotting and Northern analysis, respectively. Resistance was evaluated both in plants of T0 and T1 progenies using viruliferous whiteflies under two different inoculum pressures (1015 and 4050 whiteflies/plant). Upon inoculation with ToLCTWV using viruliferous whiteflies, various levels of phenotypic reaction were observed. No complete resistance was observed in any of the plants tested. The reaction of the transgenic tomato lines carrying full-length and truncated CP gene to ToLCTWV phenotype was (i) susceptible as non-transgenic control, (ii) delayed symptom expression, (iii) complete susceptible (from delayed symptom expression phenotype) and (iv) recovered phenotype (either plants from symptom expression as non-transgenic plants or delayed symptom expression phenotype). Dot blot quantification of the ToLCTWV using the replicase gene as a probe revealed that the recovered phenotypes accumulated a low level of ToLCTWV, and virus concentration was gradually reduced from 10 to 14 weeks postinoculation. The possible mechanisms of CP-mediated resistance are discussed. C1 [Sengoda, Venkatesan G.; Tsai, Wen-Shi; de la Pena, Robert C.; Green, Sylvia K.; Kenyon, Lawrence; Hughes, Jackie] AVRDC World Vegetable Ctr, Tainan 71499, Taiwan. [Sengoda, Venkatesan G.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Sengoda, Venkatesan G.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Sengoda, VG (reprint author), AVRDC World Vegetable Ctr, POB 42, Tainan 71499, Taiwan. EM agrivenkat@hotmail.com OI Kenyon, Lawrence/0000-0002-2739-3743 FU Agriculture Biotechnology Supporting Project II (ABSP II) FX Financial support for this work was provided through Agriculture Biotechnology Supporting Project II (ABSP II). NR 41 TC 2 Z9 2 U1 0 U2 12 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0931-1785 J9 J PHYTOPATHOL JI J. Phytopathol. PD MAY PY 2012 VL 160 IS 5 BP 213 EP 219 DI 10.1111/j.1439-0434.2012.01887.x PG 7 WC Plant Sciences SC Plant Sciences GA 921GG UT WOS:000302465500001 ER PT J AU Kidwell, KK Shelton, GB DeMacon, VL Chen, X Guy, SO Kuehner, JS Baik, BK Engle, DA Bosque-Perez, NA AF Kidwell, K. K. Shelton, G. B. DeMacon, V. L. Chen, X. Guy, S. O. Kuehner, J. S. Baik, B-K Engle, D. A. Bosque-Perez, N. A. TI Registration of 'Babe' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID PUCCINIA-STRIIFORMIS; RESISTANCE GENES AB Soft white wheat (Triticum aestivum L.) is the predominant market class of wheat produced in the Pacific Northwest of the United States. Stripe rust (caused by Puccinia striiformis Westend f. sp. tritici) is a major foliar fungal disease problem for wheat cultivars grown in the region. The objective of this research was to develop an adapted soft white spring wheat cultivar with durable resistance to stripe rust. 'Babe' (Reg. No. CV-1071, PI 656791) soft white spring wheat was developed and released in April 2009 by the Agricultural Research Center of Washington State University. Babe was tested under the experimental designations SW01003, S0400016, and WA008039, which were assigned during progressive generations of advancement. Babe is an intermediate-height, semidwarf cultivar adapted to the intermediate-to-high rainfall (>380 mm average annual precipitation), nonirrigated wheat-production regions of Washington. Babe was released based on its (i) partial resistance to the Hessian fly [Mayetiola destructor (Say)], (ii) high-temperature, adult-plant resistance to local races of stripe rust (infection type 0-3), (iii) high grain-volume weight, and (iv) high grain-yield potential in the target production region. Babe has similar or better end-use quality properties than other soft white spring cultivars currently in commercial production. C1 [Kidwell, K. K.] Washington State Univ, Coll Agr Human & Nat Resource Sci, Pullman, WA 99164 USA. [Shelton, G. B.; DeMacon, V. L.; Guy, S. O.; Kuehner, J. S.; Baik, B-K] Washington State Univ, Dep Crop & Soil Sci, Pullman, WA 99164 USA. [Chen, X.; Engle, D. A.] ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Bosque-Perez, N. A.] Univ Idaho, Dep Plant Soil & Entomol Sci, Moscow, ID 83844 USA. RP Kidwell, KK (reprint author), Washington State Univ, Coll Agr Human & Nat Resource Sci, Pullman, WA 99164 USA. EM kidwell@wsu.edu FU Washington State Grain Alliance; Agricultural Research Center of Washington State; College of Agricultural, Human and Natural Resources Sciences of Washington State University FX We greatly appreciate the dedicated assistance of the following support personnel who assisted with field testing, quality assessment, and disease screening during the development of this cultivar: Tracy Harris (WSU), Dave Wood (USDA-ARS), Kelley Richardson (USDA-ARS), and Lana Unger (Univ. of Idaho). We also thank Dr. Craig Morris, USDA-ARS Wheat Genetics, Quality, Physiology, and Disease Research Unit, Pullman, WA, for providing access to facilities, equipment, and personnel for conducting end-use quality evaluations. We are grateful to the Washington State Grain Alliance, the Agricultural Research Center of Washington State, and the College of Agricultural, Human and Natural Resources Sciences of Washington State University for providing funding for this research. NR 14 TC 2 Z9 2 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 156 EP 160 DI 10.3198/jpr2011.12.0683crc PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300005 ER PT J AU Anderson, JA Wiersma, JJ Linkert, GL Kolmer, JA Jin, Y Dill-Macky, R Wiersma, JV Hareland, GA AF Anderson, J. A. Wiersma, J. J. Linkert, G. L. Kolmer, J. A. Jin, Y. Dill-Macky, R. Wiersma, J. V. Hareland, G. A. TI Registration of 'Sabin' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID HEAD-BLIGHT RESISTANCE; F-SP TRITICI; SPRING WHEAT; MARKERS AB 'Sabin' (Reg. No. CV-1070, PI 659083) hard red spring wheat (Triticum aestivum L.) was developed by the University of Minnesota Agricultural Experiment Station and released in 2009. Sabin was tested as MN03358-4 in statewide yield trials from 2006 to 2008 and in the Uniform Hard Red Spring Wheat Regional Nursery in 2006 and 2007. Sabin was released based on its high grain yield, moderate resistance to Fusarium head blight (caused primarily by Fusarium graminearum Schwabe), and adult-plant resistance to leaf rust (Puccinia triticina Eriks.). Sabin is well adapted to hard red spring wheat growing regions in Minnesota and the Upper Midwest. C1 [Anderson, J. A.; Wiersma, J. J.; Linkert, G. L.] Univ Minnesota, Dep Agron & Plant Genet, St Paul, MN 55108 USA. [Kolmer, J. A.; Jin, Y.] ARS, USDA, St Paul, MN 55108 USA. [Dill-Macky, R.] Univ Minnesota, Dep Plant Pathol, St Paul, MN 55108 USA. [Wiersma, J. V.] Univ Minnesota, Crookston, MN 56716 USA. [Hareland, G. A.] ARS, USDA, Fargo, ND 58105 USA. RP Anderson, JA (reprint author), Univ Minnesota, Dep Agron & Plant Genet, St Paul, MN 55108 USA. EM ander319@umn.edu FU Minnesota Agricultural Experiment Station; Minnesota Wheat Research and Promotion Council; USDA-ARS [59-0790-9-025]; National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP [2006-55606-16629] FX J.A. Anderson, J.J. Wiersma, and G. L. Linkert, Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108; J.A. Kolmer and Y. Jin, USDA-ARS, St. Paul, MN 55108; R. Dill-Macky, Dep. of Plant Pathology, Univ. of Minnesota, St. Paul, MN 55108; J.V. Wiersma, Univ. of Minnesota, Crookston, MN 56716; G. A. Hareland, USDA-ARS, Fargo, ND 58105. Sabin was developed with financial support from the Minnesota Agricultural Experiment Station, the Minnesota Wheat Research and Promotion Council, and the USDA-ARS under Agreement No. 59-0790-9-025 and the National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP Grant No. 2006-55606-16629. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. Registration by CSSA. Received 30 June 2011. *Corresponding author (ander319@umn.edu). NR 20 TC 0 Z9 0 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 174 EP 179 DI 10.3198/jpr2011.06.0344crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300008 ER PT J AU Anderson, JA Wiersma, JJ Linkert, GL Kolmer, JA Jin, Y Dill-Macky, R Wiersma, JV Hareland, GA Busch, RH AF Anderson, J. A. Wiersma, J. J. Linkert, G. L. Kolmer, J. A. Jin, Y. Dill-Macky, R. Wiersma, J. V. Hareland, G. A. Busch, R. H. TI Registration of 'Tom' Wheat SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID F-SP TRITICI; SPRING WHEAT; RESISTANCE; MARKERS AB 'Tom' (Reg. No. CV-1069, PI 656383) hard red spring wheat (Triticum aestivum L.) was developed by the University of Minnesota Agricultural Experiment Station and released in 2008. Tom was tested as MN01311-A or MN01311-A-1 in statewide yield trials from 2005 to 2007 and in the Uniform Hard Red Spring Wheat Regional Nursery in 2004 and 2005. Tom was released based on its high grain yield, moderate resistance to Fusarium head blight (caused primarily by Fusarium graminearum Schwabe), and adult-plant resistance to race TTKSK (Ug99) of stem rust (Puccinia graminis Pers.:Pers. f. sp. tritici Eriks. & E. Henn.). Tom is well adapted to the hard red spring wheat growing regions in Minnesota and the Upper Midwest. C1 [Anderson, J. A.; Wiersma, J. J.; Linkert, G. L.] Univ Minnesota, Dep Agron & Plant Genet, St Paul, MN 55108 USA. [Kolmer, J. A.; Jin, Y.; Busch, R. H.] ARS, USDA, St Paul, MN 55108 USA. [Dill-Macky, R.] Univ Minnesota, Dep Plant Pathol, St Paul, MN 55108 USA. [Wiersma, J. V.] Univ Minnesota, Crookston, MN 56716 USA. [Hareland, G. A.] ARS, USDA, Fargo, ND 58105 USA. RP Anderson, JA (reprint author), Univ Minnesota, Dep Agron & Plant Genet, St Paul, MN 55108 USA. EM ander319@umn.edu FU Minnesota Agricultural Experiment Station; Minnesota Wheat Research and Promotion Council; USDA-ARS [59-0790-9-025]; National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP [2006-55606-16629] FX J.A. Anderson, J.J. Wiersma, and G. L. Linkert, Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, St. Paul, MN 55108; J.A. Kolmer, Y. Jin, and R. H. Busch, USDA-ARS, St. Paul, MN 55108; R. Dill-Macky, Dep. of Plant Pathology, Univ. of Minnesota, St. Paul, MN 55108; J.V. Wiersma, Univ. of Minnesota, Crookston, MN 56716; G. A. Hareland, USDA-ARS, Fargo, ND 58105. Tom was developed with financial support from the Minnesota Agricultural Experiment Station, the Minnesota Wheat Research and Promotion Council, and the USDA-ARS under Agreement No. 59-0790-9-025 and the National Research Initiative of USDA's Cooperative State Research, Education, and Extension Service, CAP Grant No. 2006-55606-16629. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the USDA. Registration by CSSA. Received 28 June 2011. *Corresponding author (ander319@umn.edu). NR 19 TC 6 Z9 6 U1 0 U2 4 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 180 EP 185 DI 10.3198/jpr2011.06.0339crc PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300009 ER PT J AU Mornhinweg, DW Obert, DE Carver, BF AF Mornhinweg, D. W. Obert, D. E. Carver, B. F. TI Registration of Eight Six-Rowed Feed Barley Germplasm Lines Resistant to Both Russian Wheat Aphid and Greenbug SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID BIOTYPIC DIVERSITY; SPRING BARLEY; HOMOPTERA; POPULATIONS AB STARS 1006B (Reg. No. GP-200, PI 659760), STARS 1007B (Reg. No. GP-201, PI 659761), STARS 1008B (Reg. No. GP-202, PI 659762), STARS 1009B (Reg. No. GP-203, PI 659763), STARS 1010B (Reg. No. GP-204, PI 659764), STARS 1011B (Reg. No. GP-205, PI 659765), STARS 1012B (Reg. No. GP-206, PI 659766), and STARS 1013B (Reg. No. GP-207, PI 659767), are winter, six-rowed, feed barley (Hordeum vulgare L.) germplasm lines developed cooperatively by the USDA-ARS, in Stillwater, OK, and Aberdeen, ID as sources of resistance to Russian wheat aphid [RWA; Diuraphis noxia (Kurdjumov)] and greenbug [GB; Schizaphis graminum (Rondani)]. Each resistant line is in a 'Post 90' background and comparable with 'Post 90' (PI 549081) in yield, test weight, heading date, and height in the absence of RWA and GB. Each line is highly resistant to both RWA and GB and has a wide area of adaptation ranging from irrigated production in the northwestern United States to dryland production in the southern plains, where both RWA and GB can be problematic. C1 [Mornhinweg, D. W.] ARS, USDA, Stillwater, OK 74075 USA. [Obert, D. E.] ARS, USDA, Aberdeen, ID 83210 USA. [Carver, B. F.] Oklahoma State Univ, Dep Plant & Soil Sci, Stillwater, OK 74078 USA. RP Mornhinweg, DW (reprint author), ARS, USDA, 1301N Western Rd, Stillwater, OK 74075 USA. EM Do.Mornhinweg@ars.usda.gov NR 29 TC 2 Z9 3 U1 1 U2 9 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 186 EP 189 DI 10.3198/jpr2011.09.0517crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300010 ER PT J AU Scheffler, JA Romano, GB AF Scheffler, Jodi A. Romano, Gabriela B. TI Registration of GVS1, GVS2, and GVS3 Upland Cotton Lines with Varying Gland Densities and Two Near-Isogenic Lines, GVS4 and GVS5 SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID GOSSYPIUM-HIRSUTUM L; SEED GOSSYPOL; PIGMENT GLANDS; LEVEL; INHERITANCE; PERFORMANCE; BARBADENSE; VARIETIES AB A set of five upland (Gossypium hirsutum L.) cotton germplasm lines, GVS1 (Reg. No. GP-947, PI 663886), GVS2 (Reg. No. GP-948, PI 663887), GVS3 (Reg. No. GP-949, PI 663888), GVS4 (Reg. No. GP-950, PI 663889), and GVS5 (Reg. No. GP-951, PI 663890) were developed and released by the USDA-ARS, Stoneville, MS in August 2009. These five lines include three semi-glanded lines (GVS1, GVS2, GVS3) with varying gland densities and seed gossypol content, one glanded line (GVS4), and one glandless line (GVS5); all five are in the same genetic background Stoneville 7A (PI 529228). In the F-11, the seed gossypol content of GVS1, GVS2, and GVS3 was 0.81, 0.49, and 0.50% of the total seed weight, respectively. The parental line Stoneville 7A had 1.54% and Stoneville 7A glandless had 0.00% seed gossypol. The near-isogenic lines GVS4 and GVS5 had 1.51% and 0.00% seed gossypol. GVS1, GVS2, and GVS3 have stem glanding similar to the glanded parent. Calyx glanding is normal in GVS1 and GVS3, whereas GVS2 had a 17% reduction. Boll glanding scores were 67% (GVS1), 60% (GVS2), and 59% (GVS3) of the glanded parent. In the semi-glanded lines, leaf glanding was reduced at the end of the season, but the leaves were always glanded on veins and margins. Yield and fiber properties were measured. These lines will be valuable tools for mapping, for host-plant resistance studies, and as a resource to develop low-seed-gossypol lines. C1 [Scheffler, Jodi A.] ARS, USDA, Stoneville, MS 38776 USA. [Romano, Gabriela B.] ARS, USDA, Parlier, CA 93648 USA. RP Scheffler, JA (reprint author), ARS, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM Jodi.Scheffler@ars.usda.gov FU USDA-ARS CRIS [6402-21000-031-00D] FX The authors are indebted to Michael Dowd for allowing us to use his unpublished protocol for HPLC analysis and to Scott Pelitire for analyzing a portion of the samples and successfully transferring the HPLC assay method to our lab. Thanks also to Teheshia Brooks, Gus Eifling, Shenita Harper, Shirley Howard, and Robin Jordan for valuable technical assistance and Debbie Boykin for statistical advice. This work was supported by the USDA-ARS CRIS No. 6402-21000-031-00D. NR 20 TC 2 Z9 2 U1 1 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 190 EP 194 DI 10.3198/jpr2011.10.0567crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300011 ER PT J AU Williams, WP Windham, GL AF Williams, W. Paul Windham, Gary L. TI Registration of Mp718 and Mp719 Germplasm Lines of Maize SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID AFLATOXIN; RESISTANCE; FIELD AB Maize (Zea mays L.) germplasm lines Mp718 (Reg. No. GP-581, PI 662045) and Mp719 (Reg. No. GP-582, PI 662046) were developed and released by USDA-ARS in cooperation with the Mississippi Agricultural and Forestry Experiment Station, Mississippi State, MS, as sources of resistance to aflatoxin accumulation. Aflatoxin is a potent carcinogen produced by the fungus Aspergillus flavus Link: Fries and occurs naturally in maize. Mp718 and Mp719 were developed from a cross between Mp715 (PI 614819), a line selected from 'Tuxpan' for resistance to aflatoxin accumulation, and Va35 (PI 587150), a nonstiff stalk line. These lines were evaluated for resistance to aflatoxin accumulation in a series of eight experiments conducted in Mississippi from 2004 through 2010. Neither Mp718 nor Mp719 differed from Mp715, their resistant parent, in aflatoxin accumulation, but both lines reached midsilk 13 d earlier and sustained less lodging than Mp715. Both Mp718, designated as Mp04:96 during development, and Mp719, designated as Mp04:97, exhibited excellent levels of resistance to aflatoxin accumulation in crosses with NC388. The level of resistance to aflatoxin accumulation exhibited by Mp718 and Mp719, as both germplasm lines or in single crosses, indicates that they should be reliable sources of resistance for maize breeding programs. C1 [Williams, W. Paul; Windham, Gary L.] ARS, USDA, Corn Host Plant Resistance Res Unit, Mississippi State, MS 39762 USA. RP Williams, WP (reprint author), ARS, USDA, Corn Host Plant Resistance Res Unit, 32 Creelman St, Mississippi State, MS 39762 USA. EM paul.williams@ars.usda.gov NR 13 TC 12 Z9 13 U1 0 U2 2 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 200 EP 202 DI 10.3198/jpr2011.09.0489crg PG 3 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300013 ER PT J AU Holbrook, CC Dong, WB Timper, P Culbreath, AK Kvien, CK AF Holbrook, C. Corley Dong, Weibo Timper, Patricia Culbreath, Albert K. Kvien, Craig K. TI Registration of Peanut Germplasm Line TifGP-2, a Nematode-Susceptible Sister Line of 'Tifguard' SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID ROOT-KNOT NEMATODE; SPOTTED WILT VIRUS; MELOIDOGYNE-ARENARIA; RESISTANCE; CULTIVARS; ROT AB TifGP-2 (Reg. No. GP-130, PI 663944) is a runner-type peanut (Arachis hypogaea L. subsp. hypogaea var. hypogaea) germplasm line that was released by the USDA-ARS and the Georgia Agricultural Experiment Stations in 2010. It was developed at the University of Georgia Coastal Plain Experiment Station, Tifton, GA, and was tested under the experimental designation C724-19-25. TifGP-2 is a late-generation-derived sister line of 'Tifguard', a peanut cultivar that has high resistance to both the peanut root-knot nematode [Meloidogyne arenaria (Neal) Chitwood race 1] and Tomato spotted wilt virus (TSWV; genus Tospovirus), the cause of tomato spotted wilt. During the course of developing Tifguard, we also had the opportunity to develop this closely related sister line without nematode resistance. These late-generation sister lines with and without nematode resistance can be valuable research tools to obtain a better understanding of the interactions of nematodes with other pathogens of peanut. The original breeding population was developed by hybridizing the TSWV-resistant cultivar 'C-99R' with the nematode resistant cultivar 'COAN'. C1 [Holbrook, C. Corley] ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. [Dong, Weibo] Univ Georgia, Plant Path Dep, Griffin, GA 30215 USA. [Timper, Patricia] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Culbreath, Albert K.] Univ Georgia, Plant Path Dep, Tifton, GA 31793 USA. [Kvien, Craig K.] Univ Georgia, Natl Environm Sound Prod Ag Lab, Tifton, GA 31793 USA. RP Holbrook, CC (reprint author), ARS, USDA, Crop Genet & Breeding Res Unit, POB 748,Coastal Plain Exp Stn, Tifton, GA 31793 USA. EM Corley.Holbrook@ars.usda.gov FU Georgia Peanut Commission FX The contributions and technical support of Shannon Atkinson, Brad Buchanan, Jason Golden, Vickie Hogan, Kathy Marchant, Dannie Mauldin, and Betty Tyler are gratefully acknowledged. This work was supported in part by funds from the Georgia Peanut Commission. NR 21 TC 2 Z9 3 U1 2 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 208 EP 211 DI 10.3198/jpr2011.09.0507crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300015 ER PT J AU Nelson, RL Johnson, EOC AF Nelson, R. L. Johnson, E. O. C. TI Registration of the High-Yielding Soybean Germplasm Line LG04-6000 SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID DIVERSITY AB The soybean [Glycine max (L.) Merr.] germplasm line LG04-6000 (Reg. No. GP-379, PI 664025) was developed and released by the USDA-ARS and the Illinois Agricultural Experiment Station at Urbana. LG04-6000 was derived from an F 5 plant from the cross of HS93-4118 x LG97-9912. LG97-9912 was derived from LG90-4181 x A3322. LG90-4181 is from PI 436682 x 'Lawrence' (PI 518673). PI 436682 is the Chinese cultivar 'Jilin 15', and LG04-6000 is the first germplasm released in the United States that has Jilin 15 as a progenitor. LG04-6000 is classified in early maturity group IV. In tests at 8 locations in 2006 conducted cooperatively with commercial soybean breeding companies, LG04-6000 yielded 127 kg ha(-1) more than LD00-3309. In tests at 22 locations in the Uniform Test IV in 2007 and 2008, LG04-6000 was the highest-yielding entry in the test in both years, exceeding the yield of LD00-3309, the highest-yielding check cultivar in both years, by an average of 8%. LG04-6000 is known to be susceptible to race 4 and resistant to race 7 of Phytophthora sojae Kaufmann & Gerdemann. LG04-6000 is the first example of germplasm derived from exotic parents adapted to the northern United States that has demonstrated a yield potential significantly higher than that of the best cultivars from public institutions. The combination of high yield and unique pedigree makes this line an exceptional new source of genetic diversity to improve the yield of U.S. cultivars. C1 [Nelson, R. L.; Johnson, E. O. C.] Univ Illinois, USDA, ARS, Pathol & Genet Res Unit,Dep Crop Sci, Urbana, IL 61801 USA. RP Nelson, RL (reprint author), Univ Illinois, USDA, ARS, Pathol & Genet Res Unit,Dep Crop Sci, 1101 W Peabody Dr, Urbana, IL 61801 USA. EM randall.nelson@ars.usda.gov FU United Soybean Board [9232] FX We thank the United Soybean Board for financial support for USB Project 9232, "Identification and utilization of exotic germplasm to improve soybean productivity," which provided significant resources used in the development of this line. NR 17 TC 1 Z9 2 U1 0 U2 0 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 EI 1940-3496 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 212 EP 215 DI 10.3198/jpr2011.03.0132crg PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300016 ER PT J AU Richardson, KL AF Richardson, Kelley L. TI Registration of Sugarbeet Germplasm Lines CN12-446, CN12-770, and CN72-652 with Resistance to Sugarbeet Cyst Nematode SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID POPULATIONS AB Fields infested with sugarbeet cyst nematode (SBCN; Heterodera schachtii Schmidt) are common throughout the western United States and most of the world where sugarbeet (Beta vulgaris L.) is grown. Sugarbeet cyst nematode is potentially very devastating to sugarbeet production. The sugarbeet germplasms CN12-446 (Reg. No. GP-280, PI 657939), CN12-770 (Reg. No. GP-281, PI 657940), and CN72-652 (Reg. No. GP-279, PI 657938) have high levels of SBCN resistance and were developed and released by the USDA-ARS at Salinas, CA in cooperation with the Beet Sugar Development Foundation in Denver, CO. CN12-446, CN12-770, and CN72-652 are multigerm (MM), green hypocotyl (rr), and self-fertile (S-f) lines that segregate for genetic male sterility (A-: aa). They are S-3 lines selected from two male-sterile-facilitated, random-mated populations: CN12 and CN72. CN12-446 and CN12-770 are sister S-2 lines and are more than 75% identical. Each is resistant to SBCN and powdery mildew (PM; caused by Erysiphe polygoni DC [syn. E. betae (Vanha) Weltzien]) and contains the Rz1 gene for resistance to Beet necrotic yellow vein virus, the causal agent of rhizomania. CN72-652 is susceptible to rhizomania and PM. It is unknown if CN72-652 has the same gene for SBCN resistance as CN12-446 and CN12-770. C1 ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. RP Richardson, KL (reprint author), ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM kelley.richardson@ars.usda.gov NR 13 TC 0 Z9 0 U1 1 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 216 EP 220 DI 10.3198/jpr2011.07.0349crg PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300017 ER PT J AU Jauhar, PP Peterson, TS AF Jauhar, Prem P. Peterson, Terrance S. TI Registration of DGE-2, a Durum Wheat Disomic Alien Substitution Line 1E(1A) Involving a Diploid Wheatgrass Chromosome SO JOURNAL OF PLANT REGISTRATIONS LA English DT Article ID FUSARIUM HEAD BLIGHT; ADDITION LINE; MARKERS; SSR; MAP AB The durum wheat (Triticum turgidum L., 2n = 2x = 28; AABB) alien disomic substitution 1E(1A) line DGE-2 (Reg. No. GS-171, PI 663216) was developed by the USDA-ARS, Cereal Crops Research Unit, Northern Crop Science Lab, Fargo, ND and released in 2011. Earlier, we produced and released a durum alien disomic addition line DGE-1 (2n = 30; PI 645483) that incorporated chromosome 1E of a diploid wheatgrass, Lophopyrum elongatum (Host) A. Love (2n = 2x = 14; EE). We crossed DGE-1 with 'Langdon' (CItr 13165) disomic substitution line 1D(1A) and produced F 1 hybrid derivatives with chromosomes 1A, 1D, and 1E in a single dose. We selfed these hybrid derivatives five times and screened each generation for homoeologous group-1 chromosomes 1A and 1B. At the same time, these hybrid derivatives were tested for the presence of 1E and absence of 1D. Using the chromosome-specific molecular markers Xwmc333 for identifying chromosome 1A, Xwgm18 for 1B, Xwmc147 for 1D, and Xedm17 for 1E, we isolated a stable disomic alien substitution line 1E(1A), which has been released as a new genetic stock, DGE-2. The pedigree of DGE-2 is DGE-1 (2n = 28 + 2; PI 645483)/Langdon 1D(1A)*5. This unique disomic alien substitution line DGE-2 has 2n = 28 chromosomes, in which the wheatgrass chromosome pair, 1E, compensates for the 1A pair of the durum cultivar Langdon. The disomic substitution has regular meiosis and is fertile. With a unique chromosomal constitution and a unique allele Glu-E1b, DGE-2 may be useful in basic research. C1 [Jauhar, Prem P.; Peterson, Terrance S.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. RP Jauhar, PP (reprint author), ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. EM prem.jauhar@ndsu.edu NR 9 TC 3 Z9 3 U1 0 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1936-5209 J9 J PLANT REGIST JI J. Plant Regist. PD MAY PY 2012 VL 6 IS 2 BP 221 EP 223 DI 10.3198/jpr2011.12.0662crgs PG 3 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 924LO UT WOS:000302693300018 ER PT J AU Zhang, HJ Yokoyama, WH Zhang, H AF Zhang, Huijuan Yokoyama, Wallace H. Zhang, Hui TI Concentration-dependent displacement of cholesterol in micelles by hydrophobic rice bran protein hydrolysates SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE rice bran protein (RBP); micelle; enzymatic hydrolysis; cholesterol inhibition; macroporous resins ID SERUM-CHOLESTEROL; SOY PROTEIN; BETA-LACTOGLOBULIN; RATS; PEPTIDES; RESINS; IDENTIFICATION; PURIFICATION; METABOLISM; ADSORPTION AB BACKGROUND: Rice bran, containing about 100150 g kg-1 protein, is a by-product of rice milling that has only become an available ingredient in recent years owing to the centralisation of rice milling. Rice bran, but not its protein fraction or hydrolysates, has been shown to have a hypocholesterolaemic effect. Peptides from soy, milk and other foods have been proposed to have hypocholesterolaemic effects based on their ability to lower cholesterol solubility in bile acid/phosphotidyl choline micelles. RESULTS: Rice bran protein hydrolysates (RBPHs) were prepared and investigated for their potential to lower cholesterol concentration in micelles. The RBPHs were produced by digestion using four different peptidases, alcalase 2.4L., neutrase 0.8L., papaya latex papain and porcine pancreas trypsin, and then fractionated by hydrophobicity using styrene/divinylbeneze resins. Alcalase 2.4L. produced the highest degree of hydrolysis, and the resulting hydrolysates had the highest micellar cholesterol inhibition ability in an in vitro hypocholesterolaemic test. The adsorption dynamics of four different macroporous resins, DA201-C, Sepabeads SP207 and SP825 andDiaionHP20, were determined using the Langmuir isotherm model. DA201-C had the highest adsorption capacity with an equilibrium concentration of 220 mg g-1. The hydrolysates eluted with 25, 50, 75 and 95% (v/v) ethanol lowered the micellar cholesterol concentration by 11.88, 14.76, 19.37 and 7.56% respectively. CONCLUSION: A hydrophobic fraction of RBPH had the highest inhibitory activity on micellar cholesterol, which suggests that it may have hypocholesterolaemic properties. (C) 2011 Society of Chemical Industry C1 [Zhang, Huijuan; Zhang, Hui] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China. [Zhang, Huijuan; Yokoyama, Wallace H.] USDA, Albany, CA 94710 USA. RP Zhang, H (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China. EM cerealzhanghui@yahoo.com.cn NR 29 TC 14 Z9 18 U1 3 U2 31 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-5142 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD MAY PY 2012 VL 92 IS 7 BP 1395 EP 1401 DI 10.1002/jsfa.4713 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 921HH UT WOS:000302468200010 PM 22083811 ER PT J AU Xia, ZQ Wu, SJ Pan, SK Kim, JM AF Xia, Zhenqiang Wu, Shengjun Pan, Saikun Kim, Jin Moon TI Nutritional evaluation of protein from Clanis bilineata (Lepidoptera), an edible insect SO JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE LA English DT Article DE edible insect; Clanis bilineata; protein quality; essential amino acids ID HUMAN FOOD AB BACKGROUND: This study was carried out to determine the nutritive quality of protein from Clanis bilineata (CB), an edible insect. RESULTS: The protein content of dried CB was 685.3 g kg(-1) and its essential amino acid contentwas 528.4 g kg(-1), both ofwhich are comparable to those of milk and eggs. Three groups of rats were fed diets containing either casein or CB protein (CBP) as protein source or a protein-free diet for 10 days. The nutritional parameters measured for the group fed the diet containing CBP showed a positive nitrogen balance of 1.37, a net protein retention of 2.9 and a true digestibility of 95.8%, comparable to thosemeasured for the group fed the casein diet. CONCLUSION: The results suggest that CBPmay be a suitable alternative dietary protein source for humans. (C) 2011 Society of Chemical Industry C1 [Kim, Jin Moon] USDA, Food Safety & Inspect Serv, Off Field Operat, Albany, NY 12203 USA. [Xia, Zhenqiang; Wu, Shengjun; Pan, Saikun] HuaiHai Inst Technol, Sch Marine Sci & Technol, Xinpu 222005, Peoples R China. RP Kim, JM (reprint author), USDA, Food Safety & Inspect Serv, Off Field Operat, 230 Washington Ave Extens, Albany, NY 12203 USA. EM jinkimnh@yahoo.com FU Facility Standards of Efficient Breeding of Clanis bilineata of Lianyuangang, Jiangsu, China [SF0905] FX This research was supported by Facility Standards of Efficient Breeding of Clanis bilineata of Lianyuangang, Jiangsu, China (SF0905). NR 9 TC 4 Z9 4 U1 2 U2 47 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-5142 J9 J SCI FOOD AGR JI J. Sci. Food Agric. PD MAY PY 2012 VL 92 IS 7 BP 1479 EP 1482 DI 10.1002/jsfa.4730 PG 4 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 921HH UT WOS:000302468200022 PM 22189909 ER PT J AU Roberts, RG Bischoff, JF Reymond, ST AF Roberts, Rodney G. Bischoff, Joseph F. Reymond, Stephen T. TI Differential gene expression in Alternaria gaisen exposed to dark and light SO MYCOLOGICAL PROGRESS LA English DT Article DE Alternata; ITS; Aegerolysin; Hemolysis; Host specific toxins ID HOST-SPECIFIC TOXINS; MORPHOLOGICAL SEGREGATION; SPECIES-GROUPS; RIBOSOMAL DNA; ROUGH LEMON; THEMES; FUNGI; CLASSIFICATION; INFECTORIA; PHYLOGENY AB Character states during sporulation have been used to segregate and describe many small-spored species of Alternaria, but some are not supported by published phylogenetic analyses. The conidiation response of Alternaria gaisen was characterized by selective subtractive hybridization of cDNA produced from cultures of A. gaisen grown either in total darkness or in total darkness followed by scarification and 24 h exposure to light. Transcripts or their translation products were identified using BLAST. Multiple transcripts with similarity to ORF-1 of the AM-toxin gene were obtained from the light library. L152 is a full reading frame EST in the light library whose ORF translation has similarity to the conserved domain aegerolysin (pfam06355). A set of 11 ex-type or representative isolates including A. alternata, A. gaisen, A. yaliinficiens, A. arborescens, A. tenuissima and A. brassicicola were resolved by UPGMA analysis of a partial genomic sequence (415-425 base pairs) of L152, but were not resolved by a similar analysis of ITS sequences. Furthermore, the resolved lineages of the L152 dataset were reflective of the diversity previously hypothesized by morphological evaluations of sporulation patterns. Although the ITS rDNA sequence region is generally accepted as the most likely candidate for fungal barcoding, the analysis of L152 sequences presented here resolved closely related species or species groups where other loci, including ITS, have not. Based on these results, the sequences of putative aegerolysin homologs were variable, parsimony-informative and warrant additional analyses with a broader isolate set including related genera and species. C1 [Roberts, Rodney G.; Reymond, Stephen T.] ARS, Tree Fruit Res Lab, USDA, Wenatchee, WA 98801 USA. [Bischoff, Joseph F.] USDA, Systemat Mycol & Microbiol Lab, APHIS, Beltsville, MD 20705 USA. RP Roberts, RG (reprint author), ARS, Tree Fruit Res Lab, USDA, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM rodney.roberts@ars.usda.gov NR 37 TC 6 Z9 6 U1 1 U2 12 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1617-416X EI 1861-8952 J9 MYCOL PROG JI Mycol. Prog. PD MAY PY 2012 VL 11 IS 2 BP 373 EP 382 DI 10.1007/s11557-011-0752-3 PG 10 WC Mycology SC Mycology GA 921NC UT WOS:000302483300003 ER PT J AU Kimball, BA Russell, JH Ott, PK AF Kimball, Bruce A. Russell, John H. Ott, Peter K. TI Phytochemical variation within a single plant species influences foraging behavior of deer SO OIKOS LA English DT Article ID SECONDARY METABOLITES; MONOTERPENE CONCENTRATIONS; MAMMALIAN HERBIVORES; SELECTIVE HERBIVORY; FEEDING PATTERNS; BROWSING MAMMALS; CERVUS-ELAPHUS; WOODY-PLANTS; WITHIN-PATCH; FOOD CHOICE AB To determine how black-tailed deer Odocoileus hemionus columbianus respond to phytochemical cues while browsing in heterogeneous phytochemical environments, we offered captive and free-range deer cloned rooted cuttings and seedlings of western redcedar Thuja plicata selected for varying monoterpene content. Black-tailed deer were thus allowed to browse among a controlled array of phytochemical cues in a series of experiments designed to evaluate foraging behavior at fine (within plot) and coarse (plot selection) scales. Within-plot diet selection experiments demonstrated that browse preference for individual western redcedar plants was a function of foliar monoterpene concentration. Individual plant palatability combined with momentary maximization foraging strategy promoted survival of heavily defended plants. Among-plot foraging experiments demonstrated that coarse-scale foraging preferences were strongly influenced by distributions of high monoterpene-containing western redcedar in available plots. Olfaction may play a significant role in both fine and coarse-scale browse behaviors of deer as they employ a risk-averse foraging strategy. C1 [Kimball, Bruce A.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Washington, DC 20250 USA. [Kimball, Bruce A.] Monell Chem Senses Ctr, Philadelphia, PA 19046 USA. [Russell, John H.] BC Minist Forests & Range, Res Innovat & Knowledge Management Branch, Mesachie Lake, BC V0R 2N0, Canada. [Ott, Peter K.] BC Minist Forests & Range, Res Innovat & Knowledge Management Branch, Victoria, BC V8W 9C2, Canada. RP Kimball, BA (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Washington, DC 20250 USA. EM bruce.a.kimball@aphis.usda.gov NR 64 TC 5 Z9 5 U1 1 U2 32 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0030-1299 J9 OIKOS JI Oikos PD MAY PY 2012 VL 121 IS 5 BP 743 EP 751 DI 10.1111/j.1600-0706.2011.19515.x PG 9 WC Ecology SC Environmental Sciences & Ecology GA 924TI UT WOS:000302713800011 ER PT J AU Zhu, YC West, S Liu, FX He, YP AF Zhu, Yu Cheng West, Sandy Liu, Fanny X. He, Yueping TI Interaction of proteinase inhibitors with Cry1Ac toxicity and the presence of 15 chymotrypsin cDNAs in the midgut of the tobacco budworm, Heliothis virescens (F.) (Lepidoptera: Noctuidae) SO PEST MANAGEMENT SCIENCE LA English DT Article DE proteinases; Bt; interaction; resistance; chymotrypsin; inhibitor ID BACILLUS-THURINGIENSIS TOXINS; SUBSP KURSTAKI HD-1; DELTA-ENDOTOXIN; PROTEASE INHIBITORS; OSTRINIA-NUBILALIS; INSECT RESISTANCE; COLORADO POTATO; GUT-JUICE; TRYPSIN; BINDING AB BACKGROUND: The potential development of resistance to Bacillus thuringiensis (Bt) cotton and surging of non-targeted insects is a major risk in the durability of Bt plant technology. Midgut proteinases are involved in Bt activation and degradation. Proteinase inhibitors may be used to control a wide range of insects and delay Bt resistance development. Proactive action to examine proteinase inhibitors for synergistic interaction with Bt toxin and cloning of proteinase cDNAs for RNAi is necessary to make transgenic cotton more versatile and durable. 692 RESULTS: A sublethal dose (15 ppb) of Cry1Ac, 0.5% benzamidine and 0.02% phenylmethylsulfonyl fluoride significantly suppressed midgut azocaseinase, tryptic and chymotryptic activities, and resulted in reductions in larval and pupal length and mass of Heliothis virescens. The combination of proteinase inhibitor and Bt suppressed 20-37% more larval body mass and 26-80% more enzymatic activities than the inhibitor only or Bt only. To facilitate knockdown-resistance-related proteinase genes, 15 midgut chymotrypsin cDNAs were sequenced. Most predicted chymotrypsins contained the conserved N-termini IVGG, three catalytic center residues (His, Asp and Ser), substrate specificity determinant (Ser or Gly) and cysteines for disulfide bridges. These putative chymotrypsins were separated into three distinct groups, indicating the diverse proteinases evolved in this polyphagous insect. CONCLUSION: H. virescens has evolved diverse midgut proteinase genes. Proteinase inhibitors have potential insecticidal activity, and the interaction of Bt with proteinase inhibitors is desirable for enhancing Bt toxicity and delaying resistance development. Intensive sequencing of chymotrypsin cDNAs will facilitate future functional examinations of individual roles in Bt toxicity and resistance development and facilitate targeted control using RNAi and/or proteinase inhibitors. (C) 2012 Society of Chemical Industry C1 [Zhu, Yu Cheng; West, Sandy; Liu, Fanny X.] ARS, USDA, Stoneville, MS USA. [He, Yueping] Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Inst Plant Protect & Microbiol, Hangzhou, Zhejiang, Peoples R China. RP Zhu, YC (reprint author), ARS, USDA, Stoneville, MS USA. EM yc.zhu@ars.usda.gov NR 29 TC 1 Z9 2 U1 0 U2 18 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2012 VL 68 IS 5 BP 692 EP 701 DI 10.1002/ps.2315 PG 10 WC Agronomy; Entomology SC Agriculture; Entomology GA 921HU UT WOS:000302469500006 PM 22228503 ER PT J AU Guo, ZB Zhu, YC Huang, FN Luttrell, R Leonard, R AF Guo, Zibiao Zhu, Yu Cheng Huang, Fangneng Luttrell, Randall Leonard, Rogers TI Microarray analysis of global gene regulation in the Cry1Ab-resistant and Cry1Ab-susceptible strains of Diatraea saccharalis SO PEST MANAGEMENT SCIENCE LA English DT Article DE cDNA; microarray; sugarcane borer; Diatraea saccharalis; Bt; resistance; Cry1Ab ID BACILLUS-THURINGIENSIS TOXINS; SUGARCANE BORER LEPIDOPTERA; EUROPEAN CORN-BORER; HELIOTHIS-VIRESCENS; INSECT RESISTANCE; DELTA-ENDOTOXIN; CRY1AC TOXIN; LARVAL GUT; BT TOXINS; EXPRESSION AB BACKGROUND: Extensive adoption of transgenic Bt corn in recent years for stalk borer control has increased risk of resistance evolution in the target pest populations. A Bt-resistant strain of the sugarcane borer, Diatraea saccharalis, was approximately 100-fold more tolerant to Cry1Ab toxin than the susceptible counterpart. To gain a better understanding of the molecular mechanisms of Bt resistance, the Cry1Ab-susceptible (Cry1Ab-SS) and Cry1Ab-resistant (Cry1Ab-RR) strains of D. saccharalis were subjected to a microarray analysis. RESULTS: Results showed that the expression levels of many genes were significantly different between the Cry1Ab-RR and Cry1Ab-SS strains. Microarray analysis of 7145 cDNAs revealed 384 differentially expressed genes. A total of 273 genes were significantly upregulated 2-51.6-fold, and 111 genes were significantly downregulated 2-22.6-fold in the Cry1Ab-RR strain. The upregulation of three potential resistance-related genes, coding for a glutathione S-transferase (GST), a chymotrypsin-like protease (CHY) and a lipase (LP), was confirmed using real-time PCR, indicating a reproducibility of the microarray data. Ontology analysis revealed that more than twice the number of metabolic-related genes were upregulated compared with downregulated genes with the same biological function. Up to 35.2% of the upregulated genes in the resistant strain were associated with catalytic activity, while only 9.5% of the downregulated genes were related to the same catalytic molecular function. CONCLUSION: The large portion of metabolic-or catalytic-related genes with significant upregulations indicated a potential large increase in metabolic or catalytic activities in the Cry1Ab-RR strain. This cDNA microarray gene expression data could be used to characterize and identify new genes that may be associated with Bt resistance in D. saccharalis. (C) 2012 Society of Chemical Industry C1 [Zhu, Yu Cheng] ARS, USDA, JWDSRC, SIMRU, Stoneville, MS 38776 USA. [Huang, Fangneng; Leonard, Rogers] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. RP Zhu, YC (reprint author), ARS, USDA, JWDSRC, SIMRU, Stoneville, MS 38776 USA. EM yc.zhu@ars.usda.gov NR 62 TC 7 Z9 9 U1 0 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2012 VL 68 IS 5 BP 718 EP 730 DI 10.1002/ps.2318 PG 13 WC Agronomy; Entomology SC Agriculture; Entomology GA 921HU UT WOS:000302469500009 PM 22228544 ER PT J AU Cabrera, JA Wang, D Schneider, SM Hanson, BD AF Cabrera, J. Alfonso Wang, Dong Schneider, Sally M. Hanson, Bradley D. TI Subsurface drip application of alternative fumigants to methyl bromide for controlling nematodes in replanted grapevines SO PEST MANAGEMENT SCIENCE LA English DT Article DE subsurface drip fumigation; grape replant; alternatives to methyl bromide; plant-parasitic nematodes ID PLANT-PARASITIC NEMATODES; ROOT-KNOT NEMATODE; ROOTSTOCKS; SHANK; YIELD; SOIL; 1,3-DICHLOROPROPENE; MANAGEMENT; DENSITIES; CULTIVARS AB BACKGROUND: Many California grape growers use preplant fumigation to ensure uniform and healthy grapevine establishment in replant situations. A field study was conducted to evaluate the performance of subsurface drip-applied chemical alternatives to methyl bromide on plant-parasitic nematodes, plant vigor and fruit yield during the 6 year period following replanting. RESULTS: Subsurface drip fumigation with 1,3-dichloropropene plus chloropicrin and with iodomethane plus chloropicrin had generally similar nematicide activity as methyl bromide in three grape types, while sodium azide was less effective. The combination of 1,3-dichloropropene plus chloropicrin enhanced vine vigor similarly to methyl bromide. However, all plots treated with alternative fumigants produced less fruit yield than methyl bromide over the 4 years of evaluation. CONCLUSION: Subsurface drip fumigation with alternative chemicals to methyl bromide generally provided adequate management of plant-parasitic nematodes during the vine establishment period. However, further research is required to increase the performance of alternative chemicals against other components of the replant problem, as grape yield in vines grown in the alternative treatments was lower than in methyl bromide. (C) 2011 Society of Chemical Industry C1 [Hanson, Bradley D.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Cabrera, J. Alfonso; Wang, Dong; Schneider, Sally M.; Hanson, Bradley D.] ARS, USDA, Water Management Res Unit, Parlier, CA USA. [Cabrera, J. Alfonso] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Schneider, Sally M.] ARS, USDA, Off Natl Programs, Beltsville, MD USA. RP Hanson, BD (reprint author), Univ Calif Davis, Dept Plant Sci, MS 4,1 Shields Ave, Davis, CA 95616 USA. EM bhanson@ucdavis.edu FU California Fruit Tree, Nut Tree and Grapevine Improvement Advisory Board; Sunridge Nurseries, Albemarle Corp.; Amvac Chemical Corp.; Arysta Corp.; Cal-Agri Products LLC; Dow AgroSciences LLC; Niklor Chemical Co., Inc.; Tri-Cal, Inc. FX This research was made possible by the provision of materials, services and funding from the California Fruit Tree, Nut Tree and Grapevine Improvement Advisory Board, Sunridge Nurseries, Albemarle Corp., Amvac Chemical Corp., Arysta Corp., Cal-Agri Products LLC, Dow AgroSciences LLC, Niklor Chemical Co., Inc., and Tri-Cal, Inc. Many thanks to Jim Gartung, Nancy Goodell, Ernie Leyva, Paul Neipp and Don Wade for their technical contributions to this research. NR 38 TC 6 Z9 6 U1 0 U2 21 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1526-498X J9 PEST MANAG SCI JI Pest Manag. Sci. PD MAY PY 2012 VL 68 IS 5 BP 773 EP 780 DI 10.1002/ps.2325 PG 8 WC Agronomy; Entomology SC Agriculture; Entomology GA 921HU UT WOS:000302469500015 PM 22102430 ER PT J AU Cook, D Lee, ST Pfister, JA Stonecipher, CA Welch, KD Green, BT Panter, KE AF Cook, Daniel Lee, Stephen T. Pfister, James A. Stonecipher, Clint A. Welch, Kevin D. Green, Benedict T. Panter, Kip E. TI Alkaloid Profiling as an Approach to Differentiate Lupinus garfieldensis, Lupinus sabinianus and Lupinus sericeus SO PHYTOCHEMICAL ANALYSIS LA English DT Article DE Alkaloid profiling; chemotaxonomy; crooked calf disease; lupine; Lupinus sabinianus; Lupinus garfieldensis; Lupinus sericeus; Lupinus sulphureus ID CROOKED CALF DISEASE; FORMOSUS AB Introduction - Many species in the Lupinus genus are poorly defined morphologically, potentially resulting in improper taxonomic identification. Lupine species may contain quinolizidine and/or piperidine alkaloids that can be acutely toxic and/or teratogenic, the latter resulting in crooked calf disease. Objective - To identify characteristic alkaloid profiles of Lupinus sabinianus, L. garfieldensis and L. sericeus which would aid in discriminating these species from each other and from L. sulphureus. Methods and materials - Quinolizidine and piperidine alkaloids were extracted from herbarium specimens and recent field collections of L. sabinianus, L. garfieldensis and L. sericeus. The alkaloid composition of each species was defined using GC-FID and GC-MS and compared using multivariate statistics. Results - Each of the three species investigated contained a diagnostic chemical fingerprint composed of quinolizidine and/or piperidine alkaloids. Conclusion - The alkaloid profiles of Lupinus sabinianus, L. garfieldensis and L. sericeus can be used as a tool to discriminate these species from each other and L. sulphureus as long as one considers locality of the collection in the case of L. sabinianus. Published 2011. This article is a US Government work and is in the public domain in the USA. C1 [Cook, Daniel; Lee, Stephen T.; Pfister, James A.; Stonecipher, Clint A.; Welch, Kevin D.; Green, Benedict T.; Panter, Kip E.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. RP Cook, D (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E,1400 N, Logan, UT 84341 USA. EM daniel.cook@ars.usda.gov NR 22 TC 2 Z9 2 U1 1 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0958-0344 EI 1099-1565 J9 PHYTOCHEM ANALYSIS JI Phytochem. Anal. PD MAY-JUN PY 2012 VL 23 IS 3 BP 278 EP 284 DI 10.1002/pca.1355 PG 7 WC Biochemical Research Methods; Plant Sciences; Chemistry, Analytical SC Biochemistry & Molecular Biology; Plant Sciences; Chemistry GA 919AF UT WOS:000302292300014 PM 21953740 ER PT J AU Hong, YH Song, W Lee, SH Lillehoj, HS AF Hong, Y. H. Song, W. Lee, S. H. Lillehoj, H. S. TI Differential gene expression profiles of beta-defensins in the crop, intestine, and spleen using a necrotic enteritis model in 2 commercial broiler chicken lines SO POULTRY SCIENCE LA English DT Article DE chicken; beta-defensin; cytokine; Eimeria maxima; Clostridium perfringens ID CATIONIC ANTIMICROBIAL PEPTIDES; EIMERIA-MAXIMA INFECTION; CLOSTRIDIUM-PERFRINGENS; ALPHA-DEFENSINS; IMMUNITY; GROWTH; PATHOGENESIS; TYPHIMURIUM; COCCIDIOSIS; VACCINATION AB Changes in the expression levels of avian beta-defensin (AvBD) mRNA were evaluated in necrotic enteritis (NE) disease model in 2 genetically disparate commercial broiler chicken lines: Ross and Cobb. The NE was initiated in the gut by a previously established co-infection model using oral Eimeria maxima infection followed by a Clostridium perfringens challenge. Among the 14 AvBD types examined, there was a tissue-specific expression of AvBD transcripts: AvBD1, AvBD7, and AvBD9 in the crop; AvBD8, AvBD10, and AvBD13; in the intestine and AvBD1 and AvBD7 in the spleen. The 2 different commercial broiler chicken lines showed differential gene expression patterns of AvBD transcripts following co-infection with E. maxima and C. perfringens, with R-line chickens generally showing higher expression levels than the C strain. Both chicken strains showed enhanced gene expression levels of proinflammatory cytokines, such as IL-1 beta, IL-6, IL-17F, and TNFSF15 in spleen, and TNFSF15 in intestine, whereas IL-17F was significantly increased only in the intestine of R-line chickens following NE infection. Although the exact nature of interactions between defensins and cytokines in determining the outcome of host innate immune responses to the pathogens of NE remains to be investigated, the differences in gene expression levels of beta-defensins and proinflammatory cytokines in the intestine, crop, and spleen could explain the predisposed disease resistance and susceptibility to NE in the 2 commercial broiler chicken lines. C1 [Hong, Y. H.] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. [Song, W.; Lee, S. H.; Lillehoj, H. S.] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Hong, YH (reprint author), Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, South Korea. EM yhong@cau.ac.kr RI Shin, Dong Hyuk/D-6033-2012 FU National Research Foundation of Korea (NRF); Korea government (MEST) [2011-0005177]; Rural Development Administration, Republic of Korea [PJ008084] FX This project was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST; No. 2011-0005177) and the NextGeneration BioGreen 21 Program (No. PJ008084), Rural Development Administration, Republic of Korea. The authors thank Marjorie Nichols (USDA-ARS, Beltsville, MD) for her technical contribution to this work. NR 42 TC 32 Z9 33 U1 2 U2 25 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD MAY 1 PY 2012 VL 91 IS 5 BP 1081 EP 1088 DI 10.3382/ps.2011-01948 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 925VR UT WOS:000302790500005 PM 22499864 ER PT J AU Faber, TA Dilger, RN Hopkins, AC Price, NP Fahey, GC AF Faber, T. A. Dilger, R. N. Hopkins, A. C. Price, N. P. Fahey, G. C., Jr. TI The effects of a galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex in broiler chicks challenged with Eimeria acervulina SO POULTRY SCIENCE LA English DT Article DE chick; coccidiosis; cytokine expression; Eimeria acervulina; galactoglucomannan oligosaccharide (GGMO) ID CHAIN FATTY-ACIDS; INTESTINAL IMMUNE-RESPONSES; TOTAL PARENTERAL-NUTRITION; NEONATAL PIGLET MODEL; GENE-EXPRESSION; CLOSTRIDIUM-PERFRINGENS; NUTRIENT DIGESTIBILITY; INTERFERON-GAMMA; INNATE IMMUNITY; BODY-WEIGHT AB Fermentable carbohydrates may enhance the ability of the gastrointestinal tract to defend against pathogenic infection. We hypothesized that a mannoserich, galactoglucomannan oligosaccharide-arabinoxylan (GGMO-AX) complex would positively impact immune status and prevent weight loss resulting from acute coccidiosis (Eimeria acervulina) infection of chicks. Using a completely randomized design, 1-d-old commercial broiler chicks (n = 160; 4 replications/treatment; 5 chicks/replication) were assigned to one of 4 cornsoybean meal-based diets containing supplemental GGMO-AX (0, 1, 2, or 4%) that replaced dietary cellulose. On d 9 posthatch, an equal number of chicks on each diet were inoculated with either distilled water (sham control) or E. acervulina (1 x 10(6) oocysts). All birds were euthanized on d 7 postinoculation (PI) for collection of cecal contents and duodenal tissue. Overall, BW gain of chicks was not affected by diet PI, whereas infection decreased (P < 0.01) weight gain on d 0 to 7 PI. Feed intake was not affected by dietary treatment, but infection decreased (P < 0.01) feed intake on d 0 to 7 PI. Overall, infection, but not diet, decreased (P < 0.01) G: F on d 0 to 7 PI. Cecal propionate concentrations were independently affected by infection and diet, while butyrate concentrations were affected only by infection (P - 0.02). Cecal Bifidobacterium spp. populations were affected (P < 0.01) by diet, with the 2% GGMO-AX resulting in the highest cfu/g of cecal contents (on a DM basis). Messenger RNA expression of all duodenal cytokines evaluated was affected by infection status (P <= 0.02) but not by dietary treatment alone. Supplementing 4% GGMO-AX consistently resulted in the greatest fold change in proinflammatory cytokine expression, while inhibiting antiinflammatory cytokine expression, which indicates a more robust innate immune response. Despite decreasing performance, 4% dietary GGMO-AX improved select fermentation indices and the innate intestinal immune response to an acute E. acervulina infection. C1 [Faber, T. A.; Dilger, R. N.; Fahey, G. C., Jr.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Hopkins, A. C.] Temple Inland, Diboll, TX 75941 USA. [Price, N. P.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Fahey, GC (reprint author), Univ Illinois, Dept Anim Sci, 328 Mumford Hall, Urbana, IL 61801 USA. EM gcfahey@illinois.edu OI Dilger, Ryan/0000-0003-2538-2845 NR 58 TC 12 Z9 12 U1 1 U2 6 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD MAY 1 PY 2012 VL 91 IS 5 BP 1089 EP 1096 DI 10.3382/ps.2011-01993 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 925VR UT WOS:000302790500006 PM 22499865 ER PT J AU Jones, DR Anderson, KE Guard, JY AF Jones, D. R. Anderson, K. E. Guard, J. Y. TI Prevalence of coliforms, Salmonella, Listeria, and Campylobacter associated with eggs and the environment of conventional cage and free-range egg production SO POULTRY SCIENCE LA English DT Article DE egg; housing; Salmonella; Campylobacter; Listeria ID LAYING HENS; SHELL EGGS; INTERNAL ORGANS; ENTERITIDIS; RESISTANCE; JEJUNI; ENTEROBACTERIACEAE; CONTAMINATION; COLONIZATION; PENETRATION AB There is a desire by US consumers for eggs produced by hens in alternative production systems. As the retail shell-egg market offers these products to accommodate consumer demands, additional information is needed to ensure processing methodologies result in safe eggs from all egg sources. A study was conducted to determine if there were differences in the prevalence of coliforms, Salmonella, Listeria, and Campylobacter on and within eggs and in the environment of a sister flock of conventional cage and free-range laying hens. Microbial sampling occurred approximately every 6 wk between 20 and 79 wk of age. A random sampling of typical coliform colonies produced 371 viable isolates for biochemical identification. Twenty-nine genera or species of bacteria were identified. There was a significantly greater (P < 0.0001) prevalence of Campylo-bacter in the free-range nest box swabs compared with that in the free-range grass and conventional cage swab samples (number of positives: 8 nest box, 1 grass, 0 cage). Seven isolates of Listeria innocua were detected with no significant difference in prevalence between the treatments. Isolates were associated with eggshells (2 free-range floor, 1 cage) and the free-range environment (2 nest box, 2 grass). There were 21 Salmonella isolates detected between all sample locations, with no significant difference in the prevalence of Salmonella detection between the treatments. Additional studies are needed to fully understand the effect of alternative production methods on the prevalence of pathogens and coliforms associated with nest-run eggs and the production environment. C1 [Jones, D. R.; Guard, J. Y.] ARS, Egg Safety & Qual Res Unit, USDA, Athens, GA 30605 USA. [Anderson, K. E.] N Carolina State Univ, Dept Poultry Sci, Raleigh, NC 27695 USA. RP Jones, DR (reprint author), ARS, Egg Safety & Qual Res Unit, USDA, Athens, GA 30605 USA. EM Deana.Jones@ars.usda.gov NR 32 TC 13 Z9 13 U1 2 U2 35 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD MAY 1 PY 2012 VL 91 IS 5 BP 1195 EP 1202 DI 10.3382/ps.2011-01795 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 925VR UT WOS:000302790500020 PM 22499879 ER PT J AU Zhuang, H Savage, EM AF Zhuang, H. Savage, E. M. TI Postmortem aging and freezing and thawing storage enhance ability of early deboned chicken pectoralis major muscle to hold added salt water SO POULTRY SCIENCE LA English DT Article DE broiler breast meat; water-holding capacity; deboning time; postmortem aging; frozen storage ID BREAST MEAT; MYOFIBRILLAR PROTEINS; PHOSPHATE SOLUTIONS; COOKING LOSS; CAPACITY; COLOR; QUALITY; TENDERNESS; POULTRY; PORK AB The effects of postdeboning aging and frozen storage on water-holding capacity (WHC) of chicken breast pectoralis major muscle were investigated. Broiler breast muscle was removed from carcasses either early postmortem (2 h) or later postmortem (24 h). Treatments included: no postdeboning aging; 1-d postdeboning aging at 2 degrees C, 7-d postdeboning aging (2-h deboned meat only), and 6-d storage at -20 degrees C plus 1-d thawing at 2 degrees C (freezing and thawing treatment, 2-h deboned meat only). The WHC was determined by cooking loss, drip loss, a filter paper press method (results were presented as expressible fluid), and a salt-induced swelling and centrifugation method (results were presented as percentage of salt-induced water gain). There were no differences for WHC estimated by cooking loss and expressible fluid between the treatments. Only the freezing and thawing treatment resulted in a significant increase in drip loss. The average percentage of salt-induced water gains by the 24-h deboned samples, postdeboning aged 2 h samples, and frozen 2 h sample, which did not differ from each other, were significantly higher than that by the 2-h deboned sample. These results indicate that regardless of method (carcass aging vs. postdeboning aging) and time (aging for 1 d vs. for 7 d), postmortem aging more than 1 d does not affect WHC of the early deboned samples measured by dripping, cooking, and pressing. However, postmortem carcass aging, postdeboning aging, and freezing and thawing storage can significantly enhance the ability of chicken breast meat to hold added salt water or WHC measured by the salt-induced swelling and centrifuge method. C1 [Zhuang, H.; Savage, E. M.] ARS, USDA, Russell Res Ctr, Athens, GA 30604 USA. RP Zhuang, H (reprint author), ARS, USDA, Russell Res Ctr, POB 5677, Athens, GA 30604 USA. EM hong.zhuang@ars.usda.gov NR 38 TC 7 Z9 7 U1 1 U2 12 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 J9 POULTRY SCI JI Poult. Sci. PD MAY 1 PY 2012 VL 91 IS 5 BP 1203 EP 1209 DI 10.3382/ps.2011-01819 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 925VR UT WOS:000302790500021 PM 22499880 ER PT J AU Mazzola, M Reardon, CL Brown, J AF Mazzola, Mark Reardon, Catherine L. Brown, Jack TI Initial Pythium species composition and Brassicaceae seed meal type influence extent of Pythium-induced plant growth suppression in soil SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Pythium; Species composition; Apple; Wheat; Root infection; Weed control; Biofumigation ID APPLE REPLANT DISEASE; ORGANIC PRODUCTION SYSTEMS; RHIZOCTONIA-SOLANI; PRATYLENCHUS-PENETRANS; MICROBIAL COMMUNITIES; HERBICIDAL ACTIVITY; SAPROPHYTIC GROWTH; WEED SUPPRESSION; CROPPING SYSTEMS; GREEN MANURE AB The composition of Pythium spp. communities differs among orchard soils resulting in variation in the relative importance of these pathogens to development of the biologically complex apple replant disease. Brassicaceae seed meal (SM) amendments differentially influence quantitative dynamics of Pythium populations resident to soil systems. Studies were conducted to determine the effect of initial species composition and Brassicaceae and non-Brassicaceae SM type on qualitative attributes of Pythium spp. communities and resulting effects on plant growth and disease development in two orchard soils (Soil 1 and Soil 2). Quantitatively, Pythium densities were similar between two study soils, but apple seedling mortality and suppression of wheat emergence were only observed in Soil 1. Correspondingly, application of Pythium suppressive Brassica juncea SM only improved emergence and reduced seedling mortality in Soil 1. Although Brassica napus, Glycine max and Sinapis alba SM amendments consistently elevated Pythium spp. soil densities, the corresponding effect on plant growth was more deleterious in Soil 1 than Soil 2. These differences corresponded with the initial Pythium species composition resident to the soils, with highly virulent Pythium ultimum var. ultimum dominant in Soil 1 and less virulent Pythium heterothallicum most prevalent in Soil 2. Although Pythium densities were lower in Soil 2 amended with S. alba than B. napus SM, plant damage consistently was greater in S. alba amended soil. This result corresponded with the finding that S. alba SM application preferentially elevated recovery of P. ultimum var. ultimum from Soil 2 and the roots of plants grown in this soil while less virulent species continued to dominate B. napus SM treated soil. The effect of SM type on Pythium soil densities as well as species composition should be considered to effectively employ Brassicaceae seed meals as a fertility management or soilborne disease control strategy in plant production systems. Published by Elsevier Ltd. C1 [Mazzola, Mark; Reardon, Catherine L.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Brown, Jack] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. RP Mazzola, M (reprint author), ARS, USDA, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM mark.mazzola@ars.usda.gov FU USDA NIFA FX This work was funded in part through a grant to M. Mazzola from the USDA NIFA Integrated Organic Grants Program. NR 42 TC 8 Z9 9 U1 6 U2 31 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD MAY PY 2012 VL 48 BP 20 EP 27 DI 10.1016/j.soilbio.2012.01.006 PG 8 WC Soil Science SC Agriculture GA 921AA UT WOS:000302449200003 ER PT J AU Bailey, VL Bilskis, CL Fansler, SJ McCue, LA Smith, JL Konopka, A AF Bailey, Vanessa L. Bilskis, Christina L. Fansler, Sarah J. McCue, Lee Ann Smith, Jeffrey L. Konopka, Allan TI Measurements of microbial community activities in individual soil macroaggregates SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Microscale; Aggregates; Enzyme activities; ATP ID NO-TILLAGE SOILS; FOREST SOIL; CARBON; SEQUESTRATION; PURIFICATION; FRACTIONS; ENZYMES AB The functional potential of single soil macroaggregates may provide insights into the localized distribution of microbial activities better than traditional assays conducted on bulk quantities of soil. Thus, we scaled down enzyme assays for beta-glucosidase, N-acetyl-beta-D-glucosaminidase, lipase, and leucine aminopeptidase to measure of the enzyme potential of individual macroaggregates (250-1000 mu m diameter). Across all enzymes, the smallest macroaggregates had the greatest activity and the range of enzyme activities observed in all macroaggregates supports the hypothesis that functional potential in soil may be distributed in a patchy fashion. Paired analyses of ATP as a surrogate for active microbial biomass and beta-glucosidase on the same macroaggregates suggest the presence of both extracellular beta-glucosidase functioning in macroaggregates with no detectable ATP and also of relatively active microbial communities (high ATP) that have low beta-glucosidase potentials. Studying function at a scale more consistent with microbial habitat presents greater opportunity to link microbial community structure to microbial community function. Published by Elsevier Ltd. C1 [Bailey, Vanessa L.; Bilskis, Christina L.; Fansler, Sarah J.; McCue, Lee Ann; Konopka, Allan] Pacific NW Natl Lab, Richland, WA 99354 USA. [Smith, Jeffrey L.] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. RP Bailey, VL (reprint author), Pacific NW Natl Lab, 902 Battelle Blvd,MSIN J4-18, Richland, WA 99354 USA. EM vanessa.bailey@pnnl.gov OI Bailey, Vanessa/0000-0002-2248-8890; McCue, Lee Ann/0000-0003-4456-517X FU Pacific Northwest National Laboratory; DOE [DE-AC05-76RL01830] FX This research was funded by the Pacific Northwest National Laboratory's Lab Directed Research and Development program, and is a contribution of the PNNL Microbial Communities Initiative. PNNL is operated for the DOE by Battelle under contract DE-AC05-76RL01830. NR 20 TC 9 Z9 9 U1 10 U2 51 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD MAY PY 2012 VL 48 BP 192 EP 195 DI 10.1016/j.soilbio.2012.01.004 PG 4 WC Soil Science SC Agriculture GA 921AA UT WOS:000302449200023 ER PT J AU Schechter, SP Bruns, TD AF Schechter, S. P. Bruns, T. D. TI Edaphic sorting drives arbuscular mycorrhizal fungal community assembly in a serpentine/non-serpentine mosaic landscape SO ECOSPHERE LA English DT Article DE arbuscular mycorrhizal fungi (AMF); community assembly; cpDNA; ecological sorting; edaphic factors; fungal community; McLaughlin Reserve, Lake County, California, USA; plant community; rDNA; serpentine; soil type ID COLLINSIA-SPARSIFLORA; LEUCAENA-LEUCOCEPHALA; PLANT; SOIL; AMPLIFICATION; ENVIRONMENT; ADAPTATION; DISPERSAL; DIVERSITY; GRASSES AB Serpentine soil generates distinct plant assemblages, but it is not known how this edaphically extreme environment affects arbuscular mycorrhizal fungal (AMF) assembly or how this may contribute to plant adaptation to serpentine. Our previous studies showed that serpentine and non-serpentine adapted ecotypes of Collinisa sparsiflora associates with distinct AMF assemblages, but a common garden experiment showed that this pattern was not due to host-fungal preference. We hypothesized that the observed differences in AMF associated with C. sparsiflora ecotypes was driven by edaphically defined AMF assemblages. To test this idea we employed a broader sampling of the plant community from five serpentine and five non-serpentine sites in close proximity (50-150 m between sites) and identified AMF and plant species associated with root samples by amplifying rDNA and cpDNA respectively, cloning, and sequencing. We compared AMF and plant assemblages, and measured the relative contribution of distance, plant and soil factors on AMF assembly. Analyses clearly showed that serpentine and non-serpentine AMF assemblages are distinct-with the complete absence of the non-serpentine dominant AMF taxon on serpentine. These results show strong edaphic sorting of serpentine tolerant/adapted AMF taxa in serpentine soil and indicate a strong ecological correlation between AMF and plant tolerance to serpentine soil. C1 [Schechter, S. P.; Bruns, T. D.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. RP Schechter, SP (reprint author), US Forest Serv, USDA, Pacific Southwest Res Stn, 1731 Res Pk Dr, Davis, CA 95618 USA. EM shannonp.schechter@gmail.com FU U.S. Environmental Protection Agency's Science to Achieve Results (STAR) program; University of California Natural Reserve System; Mycological Society of San Francisco; NSF [2036096, DBI1046115] FX We thank John Taylor, Bruce Baldwin and anonymous reviewers for helpful comments. We thank Nhu Nguyen, Cathy Koehler and Paul Aigner for their generous help during sampling and those involved with creating the Donald and Silvia McLaughlin Natural Reserve Research Grid, without which this work could not be done. This research has been supported by a grant from the U.S. Environmental Protection Agency's Science to Achieve Results (STAR) program, by a grant from the University of California Natural Reserve System, a grant from the Mycological Society of San Francisco, and NSF Grant #2036096 and DBI1046115 (T. D. Bruns). NR 47 TC 5 Z9 5 U1 1 U2 14 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2012 VL 3 IS 5 AR UNSP 42 DI 10.1890/ES12-00059.1 PG 24 WC Ecology SC Environmental Sciences & Ecology GA 256HY UT WOS:000327301600007 ER PT J AU Stephens, SL Boerner, REJ Moghaddas, JJ Moghaddas, EEY Collins, BM Dow, CB Edminster, C Fiedler, CE Fry, DL Hartsough, BR Keeley, JE Knapp, EE McIver, JD Skinner, CN Youngblood, A AF Stephens, Scott L. Boerner, Ralph E. J. Moghaddas, Jason J. Moghaddas, Emily E. Y. Collins, Brandon M. Dow, Christopher B. Edminster, Carl Fiedler, Carl E. Fry, Danny L. Hartsough, Bruce R. Keeley, Jon E. Knapp, Eric E. McIver, James D. Skinner, Carl N. Youngblood, Andrew TI Fuel treatment impacts on estimated wildfire carbon loss from forests in Montana, Oregon, California, and Arizona SO ECOSPHERE LA English DT Article DE carbon sequestration; fire suppression; fire surrogates; fuel management; mixed conifer; Pinus ponderosa; wildfire ID SOUTHWESTERN PONDEROSA PINE; MIXED-CONIFER FORESTS; POTENTIAL FIRE BEHAVIOR; SIERRA-NEVADA; UNITED-STATES; NATIONAL FIRE; REDUCTION TREATMENTS; WILDLAND FIRE; WOOD PRODUCTS; ECOLOGICAL RESTORATION AB Using forests to sequester carbon in response to anthropogenically induced climate change is being considered across the globe. A recent U. S. executive order mandated that all federal agencies account for sequestration and emissions of greenhouse gases, highlighting the importance of understanding how forest carbon stocks are influenced by wildfire. This paper reports the effects of the most common forest fuel reduction treatments on carbon pools composed of live and dead biomass as well as potential wildfire emissions from six different sites in four western U. S. states. Additionally, we predict the median forest product life spans and uses of materials removed during mechanical treatments. Carbon loss from modeled wildfire-induced tree mortality was lowest in the mechanical plus prescribed fire treatments, followed by the prescribed fire-only treatments. Wildfire emissions varied from 10-80 Mg/ha and were lowest in the prescribed fire and mechanical followed by prescribed fire treatments at most sites. Mean biomass removals per site ranged from approximately 30-60 dry Mg/ha; the median lives of products in first use varied considerably (from <10 to >50 years). Our research suggests most of the benefits of increased fire resistance can be achieved with relatively small reductions in current carbon stocks. Retaining or growing larger trees also reduced the vulnerability of carbon loss from wildfire. In addition, modeled vulnerabilities to carbon losses and median forest product life spans varied considerably across our study sites, which could be used to help prioritize treatment implementation. C1 [Stephens, Scott L.; Moghaddas, Jason J.; Moghaddas, Emily E. Y.; Fry, Danny L.] Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Boerner, Ralph E. J.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. [Collins, Brandon M.; Dow, Christopher B.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Edminster, Carl] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Fiedler, Carl E.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Hartsough, Bruce R.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Keeley, Jon E.] USGS Western Ecol Res Ctr, Sequoia Kings Canyon Field Stn, Three Rivers, CA 93271 USA. [Keeley, Jon E.] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA. [Knapp, Eric E.; Skinner, Carl N.] US Forest Serv, USDA, Pacific Southwest Res Stn, Redding, CA 96002 USA. [McIver, James D.] Oregon State Univ, Eastern Oregon Agr Res Ctr, Union, OR 97883 USA. [Youngblood, Andrew] US Forest Serv, USDA, Pacific NW Res Stn, La Grande, OR 97850 USA. RP Stephens, SL (reprint author), Univ Calif Berkeley, Ecosyst Sci Div, Dept Environm Sci Policy & Management, 130 Mulford Hall, Berkeley, CA 94720 USA. EM sstephens@berkeley.edu FU U.S. Joint Fire Sciences Program FX This project was partially funded by the U.S. Joint Fire Sciences Program. Special thanks to the host institutions of the experimental sites, their dedicated staffs, graduate students, post-docs, and summer field technicians who were integral to this project, and to all those who helped implement the prescribed fires. We thank two referees for comments that improved the paper. NR 92 TC 14 Z9 14 U1 0 U2 16 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD MAY PY 2012 VL 3 IS 5 AR UNSP 38 DI 10.1890/ES11-00289.1 PG 17 WC Ecology SC Environmental Sciences & Ecology GA 256HY UT WOS:000327301600003 ER PT J AU Thomas, DB AF Thomas, Donald B. TI THE AUTHORITY AND TYPES FOR THE HACKBERRY GALL PSYLLID GENUS PACHYPSYLLA (RILEY) (HEMIPTERA: PSYLLIDAE) SO ENTOMOLOGICAL NEWS LA English DT Article DE authorship; psyllid galls; cryptic species; nomina nuda ID HOMOPTERA; INSECTA AB The nomenclatural problems with the hackberry gall psyllid species names are rectified. The genus Pachypsylla Riley, 1883, type species, Psylla venusta Osten-Sacken, includes 14 nominal species: Pachypsylla venusta (Osten-Sacken, 1861); P celtidismamma Riley, 1875; P celtidisgemma Riley, 1885; P celtidisasterisca Riley, 1890; P celtidiscucurbita Riley, 1890; P celtidisglobula Riley, 1890; P celtidispubescens Riley, 1890; P celtidisumbilicus Riley, 1890; P celtidisvesiculus Riley, 1890; P celtidisinteneris Mally, 1894; P dubia Patch, 1912; P pallida Patch, 1912; P tropicala Caldwell, 1944; and P cohabitans Yang & Riemann, 2001. C1 USDA ARS, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Thomas, DB (reprint author), USDA ARS, Kika de la Garza Subtrop Agr Res Ctr, 2413 E Bus Hwy 83, Weslaco, TX 78596 USA. EM donald.thomas@ars.usda.gov NR 23 TC 0 Z9 0 U1 0 U2 0 PU AMER ENTOMOL SOC PI PHILADELPHIA PA 1900 BENJ FRANKLIN PARKWAY, PHILADELPHIA, PA 19103-1195 USA SN 0013-872X EI 2162-3236 J9 ENTOMOL NEWS JI Entomol. News PD MAY-JUN PY 2012 VL 122 IS 3 BP 279 EP 287 PG 9 WC Entomology SC Entomology GA 044WE UT WOS:000311655100011 ER PT J AU Quinones, B Swimley, MS Narm, KE Patel, RN Cooley, MB Mandrell, RE AF Quinones, Beatriz Swimley, Michelle S. Narm, Koh-Eun Patel, Ronak N. Cooley, Michael B. Mandrell, Robert E. TI O-antigen and virulence profiling of Shiga toxin-producing Escherichia coli by a rapid and cost effective DNA microarray colorimetric method SO FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY LA English DT Article DE DNA microarrays; photopolymerization; Shiga toxin; Escherichia coli; foodborne pathogen; ampliPHOX; genotyping; STEC ID ANTIMICROBIAL RESISTANCE GENES; HEMOLYTIC-UREMIC SYNDROME; OLIGONUCLEOTIDE MICROARRAY; PATHOGEN DETECTION; UNITED-STATES; PCR; INFECTIONS; STRAINS; IDENTIFICATION; HYBRIDIZATION AB Shiga toxin-producing Escherichia coli (STEC) is a leading cause of foodborne illness worldwide. The present study developed the use of DNA microarrays with the ampliPHOX colorimetric method to rapidly detect and genotype STEC strains. A low-density 30-mer oligonucleotide DNA microarray was designed to target O-antigen gene clusters of 11 E. coli serogroups (O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157) that have been associated with the majority of STEC infections. In addition, the DNA microarray targeted 11 virulence genes, encoding adhesins, cytotoxins, proteases, and receptor proteins, which have been implicated in conferring increased ability to cause disease for STEC. Results from the validation experiments demonstrated that this microarray-based colorimetric method allowed for a rapid and accurate genotyping of STEC reference strains from environmental and clinical sources and from distinct geographical locations. Positive hybridization signals were detected only for probes targeting serotype and virulence genes known to be present in the STEC reference strains. Quantification analysis indicated that the mean pixel intensities of the signal for probes targeting O-antigen or virulence genes were at least three times higher when compared to the background. Furthermore, this microarray-based colorimetric method was then employed to genotype a group of E. coli isolates from watershed sediment and animal fecal samples that were collected from an important region for leafy-vegetable production in the central coast of California. The results indicated an accurate identification of O-type and virulence genes in the tested isolates and confirmed that the ampliPHOX colorimetric method with low-density DNA microarrays enabled a fast assessment of the virulence potential of STEC using low-cost reagents and instrumentation. C1 [Quinones, Beatriz; Swimley, Michelle S.; Narm, Koh-Eun; Patel, Ronak N.; Cooley, Michael B.; Mandrell, Robert E.] USDA ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. RP Quinones, B (reprint author), USDA ARS, Western Reg Res Ctr, Produce Safety & Microbiol Res Unit, 800 Buchanan St,WAB 214L, Albany, CA 94710 USA. EM beatriz.quinones@ars.usda.gov FU USDA-Agricultural Research Service CRIS project [5325-42000-047]; National Research Initiative Competitive Grant from the USDA-National Institute of Food and Agriculture [2006-55212-16927, 2007-35212-18239] FX This work was supported by the USDA-Agricultural Research Service CRIS project number 5325-42000-047 and by the National Research Initiative Competitive Grant numbers 2006-55212-16927 and 2007-35212-18239 from the USDA-National Institute of Food and Agriculture. We thank Dr. Erica D. Dawson and Amber T. Taylor (InDevR, Inc., Boulder, CO, USA) for assistance with ampliPHOX reagents and instrumentation. We also thank Drs. E. Robert Atwill and Michelle T. Jay Russell from the University of California at Davis, the USDA/APHIS-Wildlife Services, and the California Department of Fish and Game for providing wildlife samples, NR 43 TC 11 Z9 11 U1 1 U2 4 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 2235-2988 J9 FRONT CELL INFECT MI JI Front. Cell. Infect. Microbiol. PD MAY PY 2012 VL 2 AR UNSP 61 DI 10.3389/fcimb.2012.00061 PG 10 WC Immunology; Microbiology SC Immunology; Microbiology GA 220ZU UT WOS:000324627400007 PM 22919652 ER PT J AU Beck, JJ Light, DM Gee, WS AF Beck, John J. Light, Douglas M. Gee, Wai S. TI Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Plant Biology; Issue 63; bioassay; chemoreception; electroantennography; electrophysiological response; high-throughput; host-plant volatiles; navel orangeworm; screening tool AB Plant volatiles play an important role in plant-insect interactions. Herbivorous insects use plant volatiles, known as kairomones, to locate their host plant.(1,2) When a host plant is an important agronomic commodity feeding damage by insect pests can inflict serious economic losses to growers. Accordingly, kairomones can be used as attractants to lure or confuse these insects and, thus, offer an environmentally friendly alternative to pesticides for insect control.(3) Unfortunately, plants can emit a vast number volatiles with varying compositions and ratios of emissions dependent upon the phenology of the commodity or the time of day. This makes identification of biologically active components or blends of volatile components an arduous process. To help identify the bioactive components of host plant volatile emissions we employ the laboratory-based screening bioassay electroantennography (EAG). EAG is an effective tool to evaluate and record electrophysiologically the olfactory responses of an insect via their antennal receptors. The EAG screening process can help reduce the number of volatiles tested to identify promising bioactive components. However, EAG bioassays only provide information about activation of receptors. It does not provide information about the type of insect behavior the compound elicits; which could be as an attractant, repellent or other type of behavioral response. Volatiles eliciting a significant response by EAG, relative to an appropriate positive control, are typically taken on to further testing of behavioral responses of the insect pest. The experimental design presented will detail the methodology employed to screen almond-based host plant volatiles(4,5) by measurement of the electrophysiological antennal responses of an adult insect pest navel orangeworm (Amyelois transitella) to single components and simple blends of components via EAG bioassay. The method utilizes two excised antennae placed across a "fork" electrode holder. The protocol demonstrated here presents a rapid, high-throughput standardized method for screening volatiles. Each volatile is at a set, constant amount as to standardize the stimulus level and thus allow antennal responses to be indicative of the relative chemoreceptivity. The negative control helps eliminate the electrophysiological response to both residual solvent and mechanical force of the puff. The positive control (in this instance acetophenone) is a single compound that has elicited a consistent response from male and female navel orangeworm (NOW) moth. An additional semiochemical standard that provides consistent response and is used for bioassay studies with the male NOW moth is (Z,Z)-11,13-hexdecadienal, an aldehyde component from the female-produced sex pheromone.(6-8) C1 [Beck, John J.; Light, Douglas M.; Gee, Wai S.] ARS, USDA, Washington, DC 20002 USA. RP Beck, JJ (reprint author), ARS, USDA, Washington, DC 20002 USA. EM john.beck@ars.usda.gov FU USDA-ARS CRIS Project [5325-42000-037-00D] FX This research was conducted under USDA-ARS CRIS Project 5325-42000-037-00D and with results from CRADA 58-3K95-7-1198 and TFCA 58-5325-8-419. The authors gratefully acknowledge Suterra for the gift of the (Z,Z)-11,13-hexadecadienal, B. Higbee for productive discussions, and J. Baker for technical assistance. NR 16 TC 0 Z9 0 U1 3 U2 7 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD MAY PY 2012 IS 63 AR UNSP e3931 DI 10.3791/3931 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA V36PH UT WOS:000209223000033 PM 22588282 ER PT J AU Kuethe, TH Keeney, R AF Kuethe, Todd H. Keeney, Roman TI Environmental Externalities and Residential Property Values: Externalized Costs along the House Price Distribution SO LAND ECONOMICS LA English DT Article ID SPATIAL HEDONIC APPROACH; DETERMINANTS; MARKETS; IMPACTS AB This study examines the impacts of animal agricultural facilities on the value of residential housing. Through a quantile regression framework we are able to show that the estimated price impacts are not uniform across the distribution of housing prices, and a statistically significant relationship exists only for houses at or above median price levels. These estimated price impacts also increase as the percentile increases. For comparison, the model also includes the proximity to three other waste facility types: industrial, solid, and septic. Our dataset features 14,785 single-family residential transactions in Tippecanoe County, Indiana, over the period 1993-2006. C1 [Kuethe, Todd H.] USDA, Econ Res Serv, Washington, DC USA. [Keeney, Roman] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. RP Kuethe, TH (reprint author), USDA, Econ Res Serv, Washington, DC USA. NR 23 TC 6 Z9 6 U1 2 U2 13 PU UNIV WISCONSIN PI MADISON PA SOCIAL SCIENCE BLDG, MADISON, WI 53706 USA SN 0023-7639 EI 1543-8325 J9 LAND ECON JI Land Econ. PD MAY PY 2012 VL 88 IS 2 BP 241 EP 250 PG 10 WC Economics; Environmental Studies SC Business & Economics; Environmental Sciences & Ecology GA 245QJ UT WOS:000326478600003 ER PT J AU Muller, GC Revay, EE Hogsette, JA Zeegers, T Kline, D Kravchenko, VD Schlein, Y AF Mueller, Guenter C. Revay, Edita E. Hogsette, Jerome A. Zeegers, Theo Kline, Daniel Kravchenko, Vasiliy D. Schlein, Yosef TI An annotated checklist of the horse flies (Diptera: Tabanidae) of the Sinai Peninsula Egypt with remarks on ecology and zoogeography SO ACTA TROPICA LA English DT Article DE Tabanidae; Palaearctic; Levant; Egypt; Sinai; Trypanosoma evansi; Surra; Zoogeography ID TRYPANOSOMA-EVANSI; CAMELS; JORDAN; PREVALENCE AB During a 20-year survey we routinely collected Tabanidae in the Sinai resulting in a checklist of 22 species. We recorded 2 species for the Levant (Tabanus mordax Austen, 1911 and Tabanus gratus Loew, 1858), 4 species for Egypt (Nemorius irritans (Ricardo,1901), Chrysops flavipes Meigen, 1804, Haematopotacoronata Austen, 1908 and Haematopota pallens Loew, 1871), 5 species for the Sinai (Atylotus farinosus (Szilady, 1915), Tabanus arenivagus Austen, 1920, Tabanus autumnalis Linnaeus, 1761, Haematopota minuscule Austen, 1920 and Dasyrhamphis nigritus Fabricius, 1794) for the first time and confirmed one doubtful record for the Sinai (Tabanus albifacies Loew, 1856). Furthermore we collected 10 more species that were previously known or could be presumed from the Sinai. The status of 4 species (Atylotus pallescens (Walker, 1871), Tabanus unifasciatus Loew, 1858, Tabanus politus (Walker, 1871) and Tabanus terminalis Walker, 1871) doubtfully recorded from the Sinai is discussed. For most of the 22 species, apart from zoogeographical notes, host and ecological observations are also given. (C) 2012 Elsevier B.V. All rights reserved. C1 [Mueller, Guenter C.; Schlein, Yosef] Hebrew Univ Jerusalem, Fac Med, Dept Microbiol & Mol Genet, IMRIC,Kuvin Ctr Study Infect & Trop Dis, IL-91120 Jerusalem, Israel. [Revay, Edita E.] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Anat & Cell Biol, IL-31096 Haifa, Israel. [Hogsette, Jerome A.; Kline, Daniel] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Kravchenko, Vasiliy D.] Tel Aviv Univ, Dept Zool, IL-69978 Tel Aviv, Israel. RP Muller, GC (reprint author), Hebrew Univ Jerusalem, Fac Med, Dept Microbiol & Mol Genet, IMRIC,Kuvin Ctr Study Infect & Trop Dis, IL-91120 Jerusalem, Israel. EM GunterCMuller@hotmail.com NR 46 TC 3 Z9 4 U1 0 U2 4 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X J9 ACTA TROP JI Acta Trop. PD MAY PY 2012 VL 122 IS 2 BP 205 EP 211 DI 10.1016/j.actatropica.2012.01.013 PG 7 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA 921WH UT WOS:000302508300006 PM 22289670 ER PT J AU Coudron, TA Mitchell, LC Sun, R Robertson, D Pham, NV Popham, HJR AF Coudron, T. A. Mitchell, L. C. Sun, R. Robertson, D. Pham, N. V. Popham, H. J. R. TI Dietary composition affects levels of trace elements in the predator Podisus maculiventris (Say) (Heteroptera: Pentatomidae) SO BIOLOGICAL CONTROL LA English DT Article DE Spined soldier bug; Mineral; Diet; Nutrient quality; Se; Trace elements ID RELATIVE IMPORTANCE; ARTIFICIAL DIET; WDXRF ANALYSES; SIALIS-VELATA; SPECTROMETRY; SELENIUM; WATER; HYMENOPTERA; COLEOPTERA; DROSOPHILA AB Insects require small amounts of dietary trace elements because they serve as antioxidants, enzyme co-factors and as constituents of metalloproteins. We measured the levels of 10 trace elements (Co, Cr, Cu, Fe, Mg, Mn, Mo, Ni, Se and Zn) in the predatory insect, Podisus maculiventris, reared on natural prey and artificial diet; two food sources that differed in levels of trace elements. The levels of the trace elements varied greatly in insects reared on the two food sources and differed from the concentrations in the food. We also found that most of the trace elements in the eggs were present in low concentrations and in near-equal amounts in eggs laid by females reared on either of the two food sources. The homeostatic level of most trace elements in the eggs may reflect a commitment by the female to minimize the effect of dietary variation on her offspring. We conclude that trace element levels in P. maculiventris are substantially affected by the food source and can vary significantly from levels in the food source. Knowing this will improve the ability of researchers to determine optimal dietary requirements in insects for trace elements. Additionally, achieving the optimum trace element content within beneficial insects may improve their performance when used in biological control efforts. Published by Elsevier Inc. C1 [Coudron, T. A.; Popham, H. J. R.] ARS, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. [Mitchell, L. C.] Lincoln Univ, Dept Agr & Environm Sci, Jefferson City, MO 65101 USA. [Sun, R.; Robertson, D.] Univ Missouri, Dept Chem, Columbia, MO 65211 USA. [Pham, N. V.] Plant Protect Res Inst, Hanoi, Vietnam. RP Coudron, TA (reprint author), ARS, Biol Control Insects Res Lab, USDA, 1503 S Providence Rd, Columbia, MO 65203 USA. EM tom.coudron@ars.usda.gov NR 45 TC 5 Z9 6 U1 0 U2 25 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2012 VL 61 IS 2 BP 141 EP 146 DI 10.1016/j.biocontrol.2012.01.011 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 922MG UT WOS:000302550900005 ER PT J AU Keathley, CP Harrison, RL Potter, DA AF Keathley, Craig P. Harrison, Robert L. Potter, Daniel A. TI Baculovirus infection of the armyworm (Lepidoptera: Noctuidae) feeding on spiny- or smooth-edged grass (Festuca spp.) leaf blades SO BIOLOGICAL CONTROL LA English DT Article DE Baculovirus; Peritrophic matrix; Tritrophic interaction; Trichomes; Leaf spines ID NUCLEAR-POLYHEDROSIS-VIRUS; SPODOPTERA-FRUGIPERDA LEPIDOPTERA; MULTIPLE-REGRESSION ANALYSIS; PSEUDALETIA-UNIPUNCTA; PERITROPHIC MEMBRANE; FALL ARMYWORM; MIDGUT EPITHELIUM; GRANULOSIS VIRUS; TRICHOPLUSIA NI; LARVAE AB Susceptibility of the armyworm, Mythimna unipuncta (Haworth), to infection by a baculovirus (NPV) isolated from a Kentucky armyworm population was compared on two suspected progenitors of tall fescue, Festuca mairei and Festuca arundinacea subsp. fenas, with spiny leaf margins intact or removed to test whether leaf spines abrade or puncture the peritrophic matrix (PM) and facilitate passage of virions to infection sites in midgut tissue. PCR amplification and sequencing of selected genes from the virus and phylogenetic inference from aligned sequences were carried out to identify and classify the virus. Edge spines had no effect on percentage infection or days to death of newly molted 5th instars fed spiny- or smooth-edged F. mairei leaf blades for 24 h before and after droplet feeding doses of 10(8) or 10(9) OBs ml (1). Fifth instars fed spiny- or smooth-edged grass blades had similarly undamaged PMs when viewed by scanning electron microscope. Fourth instars fed virus-treated F. arundinacea subsp. fenas leaf blades with spiny edges intact or removed did not differ in proportion infected or days to death. The food bolus moves in a liquid medium within the PM, and frass dissections showed that edge spines were often located inside a food bolus separated from the PM by non-spiny plant material rather than contiguous with the PM; therefore, friction of edge spines with the PM may have been low. These results suggest that armyworms will not be less susceptible to baculovirus infection when feeding on tall fescue cultivars with smooth leaf edges planted for improved livestock performance compared with those feeding on standard spiny-edged cultivars. We believe this to be the first study to investigate the effects of a natural physical structure on disruption of the PM and infection by a baculovirus. (C) 2012 Elsevier Inc. All rights reserved. C1 [Keathley, Craig P.; Potter, Daniel A.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. [Harrison, Robert L.] ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, USDA, Beltsville, MD 20705 USA. RP Potter, DA (reprint author), Univ Kentucky, Dept Entomol, S-225 Agr Sci Bldg N, Lexington, KY 40546 USA. EM dapotter@uky.edu RI Harrison, Robert/I-4707-2014; OI Harrison, Robert/0000-0002-8348-3874 FU USDA FAPRU [6440-21000-001-00] FX We thank C. Redmond, A. Bixby-Brosi, G. Brosi, J. Condra, E. Dobbs, J. P. Keathley, S. R. Palli, S. Clutts, D. Yu, and M. Engle for technical assistance and T. Phillips and D. Brown for help selecting and establishing grasses. This work was supported by USDA FAPRU Grant No. 6440-21000-001-00. This is paper number 11-08-079 of the Kentucky Agricultural Experiment Station. NR 52 TC 3 Z9 3 U1 2 U2 11 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD MAY PY 2012 VL 61 IS 2 BP 147 EP 154 DI 10.1016/j.biocontrol.2012.01.013 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 922MG UT WOS:000302550900006 ER PT J AU Jensen, AM Gardiner, ES Vaughn, KC AF Jensen, Anna M. Gardiner, Emile S. Vaughn, Kevin C. TI High-light acclimation in Quercus robur L. seedlings upon over-topping a shaded environment SO ENVIRONMENTAL AND EXPERIMENTAL BOTANY LA English DT Article DE Morphological plasticity; Vegetative partitioning; Shade avoidance; Irradiance; Woody sapling; Photosynthesis ID SEASON CANOPY GAP; FAGUS-SYLVATICA L; MORPHOLOGICAL ACCLIMATION; LEAF PHOTOSYNTHESIS; OAK REGENERATION; SOIL-CONDITIONS; NATURAL CANOPY; TREE SIZE; GROWTH; FORESTS AB High developmental plasticity at the seedling-level during acclimation to the light environment may be an important determinant of seedling establishment and growth in temperate broadleaf forests, especially in dense understories where spatial light availability can vary greatly. Pedunculate oak (Quercus robur L) seedlings were raised beneath a range of artificial light environments (high light, partial high light and low light) to examine morphological and photosynthetic acclimation to vertically stratified light availability. Acclimation observed at the seedling level included changes in proportional distribution of biomass and leaf area ratio to enhance either light gathering under low light availability or reduction of moisture stress under high light availability. Seedling-level acclimation was partially driven by plasticity at the flush level, but plasticity of traits determining flush morphology, such as leaf number, area, and mass, was largely controlled during bud formation rather than during shoot development. Therefore, flush-level acclimation was restricted when shoots elongated from a shaded environment into a high light environment. In contrast, traits influencing leaf-level acclimation, such as leaf thickness, specific leaf area, and pigment concentrations appeared to be driven primarily by the prevailing light environment during leaf development. The plastic response in leaf traits to light environments during shoot development enabled immediate acclimation of photosynthetic capacity to the prevailing light environment. In conclusion, oak seedlings displayed a large phenotypical plasticity on multiple levels that maximized whole seedling performance. (C) 2011 Elsevier B.V. All rights reserved. C1 [Jensen, Anna M.] Swedish Univ Agr Sci, So Swedish Forest Res Ctr, SE-23053 Alnarp, Sweden. [Gardiner, Emile S.] US Forest Serv, USDA, So Res Stn, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. [Vaughn, Kevin C.] ARS, USDA, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Jensen, AM (reprint author), Swedish Univ Agr Sci, So Swedish Forest Res Ctr, POB 49, SE-23053 Alnarp, Sweden. EM Anna.Jensen@slu.se OI Jensen, Anna Monrad/0000-0001-5113-5624 FU Stina Werner's Foundation; Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning; research program Sustainable Management in Hardwood Forests FX The authors thank Amy S. Abel for help with the plants and Brian Maxwell for help with the leaf anatomy laboratory preparations. We thank Johanna Witzell and two anonymous peers for improving this manuscript by providing constructive reviews. This work was supported in parts by Stina Werner's Foundation, the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning and the research program Sustainable Management in Hardwood Forests. NR 48 TC 6 Z9 8 U1 6 U2 30 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0098-8472 J9 ENVIRON EXP BOT JI Environ. Exp. Bot. PD MAY PY 2012 VL 78 BP 25 EP 32 DI 10.1016/j.envexpbot.2011.12.020 PG 8 WC Plant Sciences; Environmental Sciences SC Plant Sciences; Environmental Sciences & Ecology GA 910YR UT WOS:000301685000004 ER PT J AU Jensen, AB Welker, DL Kryger, P James, RR AF Jensen, Annette B. Welker, Dennis L. Kryger, Per James, Rosalind R. TI Polymorphic DNA sequences of the fungal honey bee pathogen Ascosphaera apis SO FEMS MICROBIOLOGY LETTERS LA English DT Article DE Apis mellifera; Ascosphaera; chalkbrood; DNA sequences; entomopathogenic fungi ID MEGACHILE-ROTUNDATA; DISEASE; INFECTIONS; MELLIFERA; COLONIES AB The pathogenic fungus Ascosphaera apis is ubiquitous in honey bee populations. We used the draft genome assembly of this pathogen to search for polymorphic intergenic loci that could be used to differentiate haplotypes. Primers were developed for five such loci, and the species specificities were verified using DNA from nine closely related species. The sequence variation was compared among 12 A.apis isolates at each of these loci, and two additional loci, the internal transcribed spacer of the ribosomal RNA (ITS) and a variable part of the elongation factor 1a (Ef1a). The degree of variation was then compared among the different loci, and three were found to have the greatest detection power for identifying A.apis haplotypes. The described loci can help to resolve strain differences and population genetic structures, to elucidate hostpathogen interaction and to test evolutionary hypotheses for the world's most important pollinator: the honey bee and one of its most common pathogens. C1 [Jensen, Annette B.] Univ Copenhagen, Dept Ecol & Agr, DK-1871 Frederiksberg C, Denmark. [James, Rosalind R.] Utah State Univ, Dept Biol, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. [Kryger, Per] Res Ctr Flakkebjerg, Dept Integrated Pest Management, Aarhus, Denmark. RP Jensen, AB (reprint author), Univ Copenhagen, Dept Ecol & Agr, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. EM abj@life.ku.dk RI Kryger, Per/C-1773-2013; Jensen, Annette /E-5478-2013; Jensen, Annette/G-7558-2014 OI Kryger, Per/0000-0001-5306-3230; Jensen, Annette/0000-0002-2044-2274 FU Danish National Research Foundation; Danish Council for Strategic Research FX We thank Danish beekeepers for providing chalkbrood infected mummies, and Louise Lee Munk Larsen for technical support. We also wish to thank Maria Alejandra Palacio for help with the honey bee introduction history of Latin America. This study was supported by the Danish National Research Foundation and The Danish Council for Strategic Research. NR 28 TC 2 Z9 2 U1 1 U2 15 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0378-1097 J9 FEMS MICROBIOL LETT JI FEMS Microbiol. Lett. PD MAY PY 2012 VL 330 IS 1 BP 17 EP 22 DI 10.1111/j.1574-6968.2012.02515.x PG 6 WC Microbiology SC Microbiology GA 919CL UT WOS:000302298300003 PM 22309373 ER PT J AU Evangelista, RL Isbell, TA Cermak, SC AF Evangelista, Roque L. Isbell, Terry A. Cermak, Steven C. TI Extraction of pennycress (Thlaspi arvense L.) seed oil by full pressing SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Pennycress oil; Oil extraction; Expelling ID CATALYST POISONS; SOYBEAN OIL; RAPESEED; HYDROGENATION; MOISTURE AB Pennycress is currently being developed as an oilseed crop for biofuel production. Pennycress seeds harvested from a field near Peoria, Illinois, provided our first opportunity to conduct an oil extraction study on a pilot scale. The goals of this study were to determine the effects of seed moisture and cooking on the pressing characteristics of pennycress seeds and to evaluate the quality of the oils extracted. Pennycress seeds (60 kg) with 9.5 and 16% moisture contents (MC) were cooked and dried (82-104 degrees C) using a steam-heated 3-deck laboratory seed cooker. The residence times were varied to produce cooked seeds with MCs ranging from 1.0 to 13.0%. The cooked seeds were pressed immediately using a heavy duty laboratory screw press. Pressing rate, press load, and residual oil in the press cakes were determined. The oils extracted were analyzed for solids content (foots), free fatty acid (FFA) content, color, and phosphorus (P), calcium (Ca), magnesium (Mg), and sulfur (S) contents. Pressing uncooked pennycress seeds with 9.5% MC produced press cake with 10.7% oil (db), extracting 75.1% of the oil in the seed. Cooking and drying the seeds between 3 and 4% MC provided the highest oil recovery at 86.3 and 88.0% for seeds with 9.5 and 16% starting seed MC, respectively. The pressing rates and press loads at these MCs were similar. Compared to the oil from uncooked seeds, the oils from cooked seeds had higher foots (1.55-1.73% vs. 0.52%), slightly higher FFA contents (0.40-0.46% vs. 0.30%), and slightly higher red values in AOCS RV color scale (4.1R-6.2R vs. 2.4R). Cooking increased the phosphatide content but the amount was still comparable to degummed oils. The sulfur levels in the expelled oil were higher than the amounts found in rapeseed oil and varied considerably depending on the seed moisture and the extent of cooking employed. Published by Elsevier B.V. C1 [Evangelista, Roque L.; Isbell, Terry A.; Cermak, Steven C.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Evangelista, RL (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM roque.evangelista@ars.usda.gov NR 27 TC 17 Z9 17 U1 0 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2012 VL 37 IS 1 BP 76 EP 81 DI 10.1016/j.indcrop.2011.12.003 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA 913VS UT WOS:000301906100010 ER PT J AU Cruz, VMV Dierig, DA AF Cruz, Von Mark V. Dierig, David A. TI Trends in literature on new oilseed crops and related species: Seeking evidence of increasing or waning interest SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Brassicaceae; Asteraceae; Lythraceae; Limnanthaceae; Bibliometrics; h-Index ID BIBLIOGRAPHIC ANALYSIS; SCIENCE; OIL AB Bibliographic records on eight new crop species Camelina, Crambe, Cuphea, Physaria, Limnanthes, Stokesia, Thlaspi, and Vernonia from Agricola, CAB Abstracts, Scopus, and Web of Science were analyzed for historical and recent trends on the areas of research, author distribution, and quantity and impact of publications. A total of 6786 non-redundant bibliographic records were curated and used in the analyses with about 59% of the records pertaining to the primary oilseed species. The highest number of records was found to be on Thlaspi while the least on Stokesia. Authors representing a total of 110 countries were determined to have worked in these new crops and their relative species during the past six decades with those from United States contributing about 27% in the total number of publications. There was an increase of more than ten-fold in the number of publications on new crops and relatives from 1950 to 2010 and this was paralleled by a similar increase on articles in popular and news media. The h-index values, indicating the collective impact of publications by researchers in the crop groups, show an increasing trend until the year 2000 then followed by a decline. It was determined that in recent times, there are fewer papers in the areas of chemistry, agronomy and horticulture, and more publications dealing with genetics and plant biology. Published by Elsevier B.V. C1 [Cruz, Von Mark V.; Dierig, David A.] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Cruz, VMV (reprint author), ARS, USDA, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM mark.cruz@ars.usda.gov NR 42 TC 5 Z9 5 U1 1 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2012 VL 37 IS 1 BP 141 EP 148 DI 10.1016/j.indcrop.2011.11.029 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA 913VS UT WOS:000301906100020 ER PT J AU Naranjo, SE Stefanek, MA AF Naranjo, Steven E. Stefanek, Melissa A. TI Feeding behavior of a potential insect pest, Lygus hesperus, on four new industrial crops for the arid southwestern USA SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Camelina sativa; Parthenium argentatum; Physaria fendleri; Centrapalus pauciflorus [formerly Vernonia galamensis]; Lygus hesperus; Insect behavior ID VERNONIA-GALAMENSIS; GUAYULE; HEMIPTERA; MIRIDAE; DAMAGE; OIL AB Camelina (Camelina sativa),guayule (Parthenium argentatum), lesquerella (Physaria fendleri), and vernonia (Centrapalus pauciflorus [formerly Vernonia galamensis]) are either under limited commercial production or being developed for production in the southwestern USA. Insect pests are a potential economic threat to all these new crops, with Lygus hesperus, the western tarnished plant bug, among the most prominent due to its regional abundance and propensity to feed on reproductive plant tissue. The objectives of this study were to establish baseline data on the feeding behavior and potential impact of L hesperus on camelina, guayule, lesquerella and vernonia. Behavioral observations of adult females and males, and nymphs of this insect were made in the laboratory. Insects spent approximate to 35% of their time either probing (=tasting) or feeding on various reproductive and vegetative tissues of guayule, lesquerella or vernonia, but only 20% on camelina. When insects did probe and feed they preferred reproductive tissues, primarily flowers and siliques/achenes, and there were differences in these behaviors relative to crop but not generally to insect stage or sex. Insects probed and fed more on flower tissue of guayule and vernonia compared with camelina and lesquerella, and more on siliques of lesquerella compared with achenes of vernonia. When probing and feeding on vegetative tissue, there was generally a preference for stems compared with leaves in all crops except guayule. Results show that L hesperus will readily feed on the economically important tissues of all crops, and although research has shown that this feeding did not consistently affect lesquerella yield, further work will be needed to determine if such feeding poses a risk to commercial production of camelina, guayule or vemonia. Published by Elsevier B.V. C1 [Naranjo, Steven E.; Stefanek, Melissa A.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Naranjo, SE (reprint author), ARS, USDA, Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM steve.naranjo@ars.usda.gov NR 16 TC 3 Z9 3 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2012 VL 37 IS 1 BP 358 EP 361 DI 10.1016/j.indcrop.2011.12.020 PG 4 WC Agricultural Engineering; Agronomy SC Agriculture GA 913VS UT WOS:000301906100052 ER PT J AU Cermak, DM Cermak, SC Deppe, AB Durham, AL AF Cermak, Diana M. Cermak, Steven C. Deppe, Amy B. Durham, Amber L. TI Novel alpha-hydroxy phosphonic acids via castor oil SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Castor oil; Ricinoleic acid; alpha-Hydroxy phosphonates; alpha-Hydroxy phosphonic acids ID LESQUERELLA; OXIDATION; ESTERS AB Hydroxy fatty acids (HFAs) have found a number of uses in today's market, with uses ranging from materials to pharmaceuticals. Castor oil has served as a source of a versatile HFA; its principle component, ricinoleic acid, can be isolated from castor oil and has been modified extensively for a number of applications. Additionally, alpha-hydroxy phosphonates and their corresponding phosphonic acids are a functional moiety that have been shown to display a wide variety of biological activities, as enzyme inhibitors, pesticides, antibiotics and anti-cancer therapeutics. We were interested in combining these two functionalities. HFAs modified to produce alpha-hydroxy phosphonates and phosphonic acids, as potentially biologically active molecules. We have accomplished the synthesis of two families of alpha-hydroxy phosphonic acids based on ricinoleic acid: a family that retains the cis alkene found in ricinoleic acid to produce an unsaturated alpha-hydroxy phosphonic acid and one where the alkene has undergone hydrogenation to produce a saturated alpha-hydroxy phosphonic acid. These compounds have been produced in high yields and high purity, and the synthesis of these compounds is reported in this manuscript. (C) 2012 Elsevier B.V. All rights reserved. C1 [Cermak, Diana M.] Knox Coll, Dept Chem, Galesburg, IL 61401 USA. [Cermak, Steven C.; Deppe, Amy B.; Durham, Amber L.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Cermak, DM (reprint author), Knox Coll, Dept Chem, 2 E South St, Galesburg, IL 61401 USA. EM dcermak@knox.edu; Steven.Cermak@ars.usda.gov FU Knox College Mellon Foundation FX The authors are grateful to Dr. Karl Vermillion (USDA) for performing all the NMR experiments. Knox College Mellon Foundation Faculty Grant Program for financial support for this research project. NR 15 TC 4 Z9 4 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2012 VL 37 IS 1 BP 394 EP 400 DI 10.1016/j.indcrop.2011.12.018 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA 913VS UT WOS:000301906100057 ER PT J AU Chundawat, SPS Chang, LP Gunawan, C Balan, V McMahan, C Dale, BE AF Chundawat, Shishir P. S. Chang, Linpei Gunawan, Christa Balan, Venkatesh McMahan, Colleen Dale, Bruce E. TI Guayule as a feedstock for lignocellulosic biorefineries using ammonia fiber expansion (AFEX) pretreatment SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Guayule; AFEX pretreatment; Enzymatic hydrolysis; Cellulosic biofuels ID SACCHAROMYCES-CEREVISIAE 424A(LNH-ST); CELLULOSIC ETHANOL-PRODUCTION; CORN STOVER; BIOMASS; PRODUCTS AB Natural rubber latex extraction from guayule leaves behind greater than 90% (by weight) of agricultural residue as a feedstock suitable for conversion to biofuels via a thermochemical or biochemical route. Untreated guayule shrub and bagasse (after latex extraction) has shown to be very recalcitrant to enzymatic hydrolysis, necessitating application of a chemical pretreatment to enhance cellulase accessibility. The objective of this work was to carry out detailed compositional analysis, ammonia fiber expansion (AFEX(1)) pretreatment, enzymatic hydrolysis and ethanol fermentation for various guayule-derived biomass fractions. Plant feedstocks tested were derived from two sources; (a) a mature 2007 AZ-2 whole guayule shrub plant obtained from USDA/ARS(2) research fields, and (b) the guayule latex-extracted commercial grade bagasse (62505) from Yulex Corporation. Compositional analysis and enzymatic hydrolysis were carried out using standard NREL3 protocols (www.nrel.gov/biomass/analytical_procedures.html). AFEX pretreatment was carried out using concentrated ammonium hydroxide at elevated temperatures for desired residence times in a pressurized reactor. Yeast fermentations on biomass hydrolyzates were carried out micro-aerobically using Saccharomyces cerevisiae (424A strain) in shake flasks. AFEX pretreatment was found to substantially improve overall enzymatic digestibility by 4-20 fold for both untreated guayule shrub and latex-extracted bagasse. Maximum glucan and xylan conversion achieved for the latex-extracted bagasse was 40% and 50%, respectively. The yeast was readily able to ferment both glucose and xylose to ethanol from the guayule bagasse hydrolyzate with or without external nutrient supplementation (i.e., yeast extract and tryptone). Our results highlight the possible utilization of guayule as a feedstock for lignocellulosic refineries co-producing natural rubber latex and biofuels. However, further process improvements (e.g., lignin/resin extraction and cellulose decrystallization using a modified AFEX process) are necessary to increase the effectiveness of ammonia-based pretreatments for further enhancing enzymatic digestibility of guayule-derived hardwood biomass. (C) 2011 Elsevier B.V. All rights reserved. C1 [Chundawat, Shishir P. S.; Chang, Linpei; Gunawan, Christa; Balan, Venkatesh; Dale, Bruce E.] Michigan State Univ, Dept Chem Engn & Mat Sci, BCRL, E Lansing, MI 48824 USA. [Chundawat, Shishir P. S.; Balan, Venkatesh; Dale, Bruce E.] GLBRC, E Lansing, MI USA. [McMahan, Colleen] ARS, USDA, Western Reg Res Lab, Albany, CA USA. RP Chundawat, SPS (reprint author), 3900 Collins Rd,Suite 1045, Lansing, MI 48910 USA. EM chundawa@msu.edu OI Chundawat, Shishir/0000-0003-3677-6735 FU MSU SPG; DOE Great Lakes Bioenergy Research Center (DOE Office of Science) [BER DE-FC02-07ER64494] FX We thank Rajesh Gupta (Auburn University) for carrying out compositional analyses, and Alex Arceneaux (USDA-ARS) for performing the solvent extractions of guayule samples. The commercial bagasse used in this study was provided by Yulex Corporation, Maricopa, AZ. Dr. Terry Coffelt, USDA-ARS, USALARC, Maricopa, AZ generously provided guayule plants and a review of the manuscript. This work was funded in part by the MSU SPG grant and the DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER DE-FC02-07ER64494). NR 27 TC 8 Z9 8 U1 1 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2012 VL 37 IS 1 BP 486 EP 492 DI 10.1016/j.indcrop.2011.07.025 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA 913VS UT WOS:000301906100071 ER PT J AU Snider, JL Raper, RL Schwab, EB AF Snider, John L. Raper, Randy L. Schwab, Eric B. TI The effect of row spacing and seeding rate on biomass production and plant stand characteristics of non-irrigated photoperiod-sensitive sorghum (Sorghum bicolor (L.) Moench) SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Bioenergy; Photoperiod-sensitive sorghum; Row spacing; Seeding rate; Plant population; Biomass ID GRAIN-SORGHUM; CELLULOSIC ETHANOL; FORAGE SORGHUM; SWEET SORGHUM; YIELD; FEEDSTOCK; QUALITY; PERFORMANCE; POPULATION; WILLOW AB To evaluate row spacing and seeding rate effects on yield and plant stand characteristics of high-biomass sorghum, a photoperiod-sensitive cultivar was sown at three different row spacings (76, 38, and 19 cm) and seeding rates (218,000, 306,000, and 393,000 seeds ha(-1) for one site-year and 116,000, 204,000, and 291,000 seeds ha(-1) for three site-years) from 2009 to 2010 in Alabama and Arkansas, USA. Measurements included above-ground dry matter production, plant height, stem density, and stem diameter. Narrower row spacing (i.e. 19 cm) produced the highest biomass for all site-years. Increasing seeding rate did not affect yield for three of the site-years, and decreased yield for one. The 19 cm row spacing produced the highest stem densities. Plant height increased with increasing seeding rates at one site and decreased with higher seeding rates at another site. At one location, stem diameter declined as seeding rates and stein density increased. It was concluded that narrower row spacing (19 cm) provides the maximum yield benefit by significantly increasing stem density, and low seeding rates (116,000 seeds ha(-1)) are preferable because higher seeding rates do not positively affect yield and may cause morphological changes (i.e. taller plants with thinner stems) conducive to lodging. Published by Elsevier B.V. C1 [Snider, John L.; Raper, Randy L.] ARS, USDA, Dale Bumpers Small Farms Res Ctr, Booneville, AR 72927 USA. [Schwab, Eric B.] Nat Resources Conservat Serv, USDA, Auburn, AL 36830 USA. RP Snider, JL (reprint author), ARS, USDA, Dale Bumpers Small Farms Res Ctr, 6883 S State Hwy 23, Booneville, AR 72927 USA. EM john.snider@ars.usda.gov NR 31 TC 7 Z9 10 U1 4 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 J9 IND CROP PROD JI Ind. Crop. Prod. PD MAY PY 2012 VL 37 IS 1 BP 527 EP 535 DI 10.1016/j.indcrop.2011.07.032 PG 9 WC Agricultural Engineering; Agronomy SC Agriculture GA 913VS UT WOS:000301906100077 ER PT J AU Sim, SB Mattsson, M Feder, JL Cha, DH Yee, WL Goughnour, RB Linn, CE Feder, JL AF Sim, S. B. Mattsson, M. Feder, Jasmine L. Cha, D. H. Yee, W. L. Goughnour, R. B. Linn, C. E., Jr. Feder, Jeffrey L. TI A field test for host fruit odour discrimination and avoidance behaviour for Rhagoletis pomonella flies in the western United States SO JOURNAL OF EVOLUTIONARY BIOLOGY LA English DT Article DE apple maggot fly; ecological speciation; field trapping; flight tunnel; fruit odour discrimination; olfaction ID APPLE MAGGOT FLY; SPECIES DIPTERA TEPHRITIDAE; SYMPATRIC SPECIATION; GENETIC DIFFERENTIATION; PHYTOPHAGOUS INSECTS; WASHINGTON-STATE; MATING BEHAVIOR; PLANT USE; VOLATILES; HAWTHORN AB Prezygotic isolation due to habitat choice is important to many models of speciation-with-gene-flow. Habitat choice is usually thought to occur through positive preferences of organisms for particular environments. However, avoidance of non-natal environments may also play a role in choice and have repercussions for post-zygotic isolation that preference does not. The recent host shift of Rhagoletis pomonella (Diptera: Tephritidae) from downy hawthorn, Crataegus mollis, to introduced apple, Malus domestica, in the eastern United States is a model for speciation-with-gene-flow. However, the fly is also present in the western United States where it was likely introduced via infested apples = 60 years ago. R. pomonella now attacks two additional hawthorns in the west, the native C. douglasii (black hawthorn) and the introduced C. monogyna (English ornamental hawthorn). Flight tunnel tests have shown that western apple-, C. douglasii- and C. monogyna-origin flies all positively orient to fruit volatile blends of their respective natal hosts in flight tunnel assays. Here, we show that these laboratory differences translate to nature through field-trapping studies of flies in the state of Washington. Moreover, western R. pomonella display both positive orientation to their respective natal fruit volatiles and avoidance behaviour (negative orientation) to non-natal volatiles. Our results are consistent with the existence of behaviourally differentiated host races of R. pomonella in the west. In addition, the rapid evolution of avoidance behaviour appears to be a general phenomenon for R. pomonella during host shifts, as the eastern apple and downy hawthorn host races also are antagonized by non-natal fruit volatiles. C1 [Sim, S. B.; Feder, Jasmine L.; Feder, Jeffrey L.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA. [Mattsson, M.] Portland State Univ, Dept Biol, Portland, OR 97207 USA. [Cha, D. H.; Linn, C. E., Jr.] Cornell Univ, Dept Entomol, Geneva, NY USA. [Cha, D. H.; Yee, W. L.] USDA ARS, Yakima Agr Res Lab, Wapato, WA USA. [Goughnour, R. B.] Washington State Univ, Res & Extens Unit, Vancouver, WA USA. RP Feder, JL (reprint author), Univ Notre Dame, Dept Biol Sci, 290C Galvin Life Sci Bldg, Notre Dame, IN 46556 USA. EM feder.2@nd.edu; feder.2@nd.edu RI Cha, Dong/A-3977-2013 FU NSF; USDA; Washington Tree Fruit Research Commission; Washington State Commission on Pesticide Registration FX The authors would like to thank the Clark County, Washington, 78th street Heritage Farm, the Washington State University Research and Extension Unit, Vancouver, Blair Wolfley, Doug Stienbarger, Terry Porter and Kathleen Rogers for their support and assistance on the project. We also appreciate the effort of Tom Glen and Scott Tracy at Suterra LLC (Bend, Oregon, USA) in making the wax fruit odour lures on short notice and Mike Chong at the University of Waterloo for the synthesis of DMNT. Special thanks also to Tom Powell, Glen Hood Tracy Arcella, Gilbert St. Jean and Scott Egan for helpful discussions, insight, advice and constructive criticism of the paper. This work was supported in part by grants to JLF from the NSF and the USDA, and to WLY by the Washington Tree Fruit Research Commission and Washington State Commission on Pesticide Registration. NR 51 TC 12 Z9 12 U1 0 U2 35 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1010-061X J9 J EVOLUTION BIOL JI J. Evol. Biol. PD MAY PY 2012 VL 25 IS 5 BP 961 EP 971 DI 10.1111/j.1420-9101.2012.02489.x PG 11 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 922MH UT WOS:000302551000016 PM 22435643 ER PT J AU Briggs, RE Tabatabai, LB Tatum, FM AF Briggs, Robert E. Tabatabai, Louisa B. Tatum, Fred M. TI Mucosal and parenteral vaccination against pneumonic pasteurellosis in cattle with a modified-live in-frame lktA deletion mutant of Mannheimia haemolytica SO MICROBIAL PATHOGENESIS LA English DT Article DE Bacteria; Mannheimia haemolytica; Respiratory disease; Pneumonia; Bovine; Cattle; Calf; Plasmid ID BOVINE RESPIRATORY-DISEASE; TRANSPORTED CALVES; SEROTYPE A1; COLONIZATION; PROTECTION; LEUKOTOXIN; TONSILS; NASOPHARYNX; GENERATION; INHIBITION AB A new temperature-conditional shuttle vector, pBB80C, was constructed and utilized to generate an in-frame deletion in the leukotoxin structural gene of Mannheimia haemolytica serotype 1. Culture supernatants from the mutant contained no detectable cytotoxicity to BL-3 lymphocyte targets, and contained a new protein with an approximate molecular weight of 66 kDa which was reactive to anti-leukotoxin monoclonal antibody. No protein reactive to anti-LktA monoclonal antibody was detected at the molecular weight 100-105 kDa of native LktA. Calves vaccinated mucosally by top-dressing the live mutant onto feed, or parenterally by subcutaneous injection, were resistant to virulent challenge with the parent strain. Serologic antibody response, reduction in lung lesion, and reduction in pulmonary infectious load were greater among calves mucosally vaccinated than those which were vaccinated by injection. Published by Elsevier Ltd. C1 [Briggs, Robert E.; Tabatabai, Louisa B.; Tatum, Fred M.] USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Briggs, RE (reprint author), USDA, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM robert.briggs@ars.usda.gov NR 26 TC 2 Z9 2 U1 1 U2 9 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0882-4010 J9 MICROB PATHOGENESIS JI Microb. Pathog. PD MAY PY 2012 VL 52 IS 5 BP 302 EP 308 DI 10.1016/j.micpath.2012.02.008 PG 7 WC Immunology; Microbiology SC Immunology; Microbiology GA 920YC UT WOS:000302444200007 PM 22401911 ER PT J AU Greenstone, MH Weber, DC Coudron, TA Payton, ME Hu, JS AF Greenstone, Matthew H. Weber, Donald C. Coudron, Thomas A. Payton, Mark E. Hu, Jing S. TI Removing external DNA contamination from arthropod predators destined for molecular gut-content analysis SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE DNA decontamination; molecular gut-content analysis; sampling ID COLORADO POTATO BEETLE; POLYMERASE-CHAIN-REACTION; GENERALIST PREDATORS; ALTERNATIVE PREY; APHID PREDATION; CONTENT ELISA; FOOD WEBS; PCR; TRACKING; SPIDERS AB Ecological research requires large samples for statistical validity, typically hundreds or thousands of individuals, which are most efficiently gathered by mass-collecting techniques. For the study of interspecific interactions, molecular gut-content analysis enables detection of arthropod predation with minimal disruption of community interactions. Field experiments have demonstrated that standard mass-collection methods, such as sweep netting, vacuum sampling and foliage beating, sometimes lead to contamination of predators with nontarget DNA, thereby compromising resultant gut-content data. We deliberately contaminated immature Coleomegilla maculata and Podisus maculiventris that had been fed larvae of Leptinotarsa decemlineata by topically applying homogenate of the alternate prey Leptinotarsa juncta. We then attempted to remove contaminating DNA by washing in ethanol or bleach. A 40-min wash with end-over-end rotation in 80% EtOH did not reliably reduce external DNA contamination. Identical treatment with 2.5% commercial bleach removed most externally contaminating DNA without affecting the detectability of the target prey DNA in the gut. Use of this bleaching protocol, perhaps with minor modifications tailored to different predatorprey systems, should reliably eliminate external DNA contamination, thereby alleviating concerns about this possible source of cross-contamination for mass-collected arthropod predators destined for molecular gut-content analysis. C1 [Greenstone, Matthew H.; Weber, Donald C.; Hu, Jing S.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Coudron, Thomas A.] ARS, USDA, Biol Control Insects Res Lab, Columbia, MO 65203 USA. [Payton, Mark E.] Oklahoma State Univ, Dept Stat, Stillwater, OK 74078 USA. RP Greenstone, MH (reprint author), ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. EM matt.greenstone@ars.usda.gov NR 37 TC 7 Z9 9 U1 3 U2 45 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1755-098X J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD MAY PY 2012 VL 12 IS 3 BP 464 EP 469 DI 10.1111/j.1755-0998.2012.03112.x PG 6 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 922MZ UT WOS:000302552900011 PM 22268594 ER PT J AU Baisakh, N RamanaRao, MV Rajasekaran, K Subudhi, P Janda, J Galbraith, D Vanier, C Pereira, A AF Baisakh, Niranjan RamanaRao, Mangu V. Rajasekaran, Kanniah Subudhi, Prasanta Janda, Jaroslav Galbraith, David Vanier, Cheryl Pereira, Andy TI Enhanced salt stress tolerance of rice plants expressing a vacuolar H plus -ATPase subunit c1 (SaVHAc1) gene from the halophyte grass Spartina alterniflora Loisel SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE gene expression; halophyte; rice; salt tolerance; vacuolar ATPase ID MESEMBRYANTHEMUM-CRYSTALLINUM; PLASMA-MEMBRANE; SALINITY TOLERANCE; DIFFERENTIAL EXPRESSION; ARABIDOPSIS-DET3 MUTANT; NA+/H+ ANTIPORT; PROTON PUMPS; CROP PLANTS; GROWTH; REVEALS AB The physiological role of a vacuolar ATPase subunit c1 (SaVHAc1) from a halophyte grass Spartina alterniflora was studied through its expression in rice. The SaVHAc1-expressing plants showed enhanced tolerance to salt stress than the wild-type plants, mainly through adjustments in early stage and preparatory physiological responses. In addition to the increased accumulation of its own transcript, SaVHAc1 expression led to increased accumulation of messages of other native genes in rice, especially those involved in cation transport and ABA signalling. The SaVHAc1-expressing plants maintained higher relative water content under salt stress through early stage closure of the leaf stoma and reduced stomata density. The increased K+/Na+ ratio and other cations established an ion homoeostasis in SaVHAc1-expressing plants to protect the cytosol from toxic Na+ and thereby maintained higher chlorophyll retention than the WT plants under salt stress. Besides, the role of SaVHAc1 in cell wall expansion and maintenance of net photosynthesis was implicated by comparatively higher root and leaf growth and yield of rice expressing SaVHAc1 over WT under salt stress. The study indicated that the genes contributing toward natural variation in grass halophytes could be effectively manipulated for improving salt tolerance of field crops within related taxa. C1 [Baisakh, Niranjan; RamanaRao, Mangu V.; Subudhi, Prasanta] Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. [Rajasekaran, Kanniah] Agr Res Serv, So Reg Res Ctr, USDA, New Orleans, LA USA. [Janda, Jaroslav; Galbraith, David] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA. [Vanier, Cheryl] Univ Nevada, Las Vegas, NV 89154 USA. [Pereira, Andy] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. RP Baisakh, N (reprint author), Louisiana State Univ, Ctr Agr, Sch Plant Environm & Soil Sci, Baton Rouge, LA 70803 USA. EM nbaisakh@agcenter.lsu.edu FU USDA-CSREES FX The technical assistance of Greg Ford, SRRC, USDA-ARS is duly acknowledged. This work was financially supported by USDA-CSREES. This manuscript is approved for publication by the Director of Louisiana Agricultural Experiment Station as MS#2011-306-6572. NR 57 TC 34 Z9 38 U1 4 U2 36 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1467-7644 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD MAY PY 2012 VL 10 IS 4 BP 453 EP 464 DI 10.1111/j.1467-7652.2012.00678.x PG 12 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA 919AA UT WOS:000302291800008 PM 22284568 ER PT J AU Chen, XL Jahns, L Gittelsohn, J Wang, YF AF Chen, Xiaoli Jahns, Lisa Gittelsohn, Joel Wang, Youfa TI Who is missing the message? Targeting strategies to increase food label use among US adults SO PUBLIC HEALTH NUTRITION LA English DT Article DE Food label; Nutrition knowledge; Sociodemographic; Obesity; Ethnicity ID NUTRITION EXAMINATION SURVEY; BODY-MASS INDEX; NATIONAL-HEALTH; CHRONIC DISEASE; DIETARY-INTAKE; NEW-ZEALAND; INFORMATION; AUSTRALIA; QUALITY; OBESITY AB Objective: To evaluate the associations between sociodemographic and psychosocial characteristics and food label (FL) use in US adults. Design: Data from the 1994-1996 Continuing Survey of Food Intakes by Individuals and the Diet and Health Knowledge Survey were used. High socio-economic status (SES) was defined as >high school education and poverty-income ratio (PIR) <350%, low SES as, = 25 kg/m(2)), those who perceived their weight 'about right' were 51% less likely to use FL than those perceiving themselves as overweight. Conclusions: Men, especially black men, women of low SES, rural residents and overweight Americans with inaccurate self-perception of body weight are less likely to use FL and should be targeted for increased intervention. C1 [Chen, Xiaoli; Gittelsohn, Joel; Wang, Youfa] Johns Hopkins Bloomberg Sch Publ Hlth, Ctr Human Nutr, Dept Int Hlth, Baltimore, MD 21205 USA. [Jahns, Lisa] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND USA. RP Wang, YF (reprint author), Johns Hopkins Bloomberg Sch Publ Hlth, Ctr Human Nutr, Dept Int Hlth, 615 N Wolfe St, Baltimore, MD 21205 USA. EM ywang@jhsph.edu OI Jahns, Lisa/0000-0002-1828-6962 FU National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases (NIH/NIDDK) [R01DK81335-01A1] FX The study was supported by the National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases (NIH/NIDDK, R01DK81335-01A1). The authors declare no conflict of interest. X.C. and Y.W. contributed to the conceptualization, statistical analysis, literature review, interpretation of results, and write-up of the manuscript. L.J. and J.G. interpreted the results and revised the manuscript. The authors thank Dr May Beydoun for her technical assistance in working on the data sets. NR 25 TC 12 Z9 12 U1 1 U2 16 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD MAY PY 2012 VL 15 IS 5 BP 760 EP 772 DI 10.1017/S1368980011002242 PG 13 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA 919XP UT WOS:000302365800003 PM 21899793 ER PT J AU Nord, M AF Nord, Mark TI How much does the Supplemental Nutrition Assistance Program alleviate food insecurity? Evidence from recent programme leavers SO PUBLIC HEALTH NUTRITION LA English DT Article DE Food security; Food insecurity; Supplemental; Nutrition; Assistance Program; SNAP ID STAMP PROGRAM AB Objective: To estimate the effect of the US Supplemental Nutrition Assistance Program (SNAP) on the food security (consistent access to adequate food) of recipients, net of the effect of the self-selection of more food-needy households into the programme. Design: The food security of current SNAP recipients and recent leavers is compared in cross-sectional survey data, adjusting for economic and demographic differences using multivariate logistic regression methods. A similar analysis in 2-year longitudinal panels provides additional control for selection on unobserved variables based on food security status in the previous year. Setting: Household survey data collected for the US Department of Agriculture by the US Census Bureau. Subjects: Households interviewed in the Current Population Survey Food Security Supplements from 2001 to 2009. Results: The odds of very low food security among households that continued on SNAP through the end of a survey year were 28% lower than among those that left SNAP prior to the 30-d period during which food security was assessed. In 2-year panels with controls for the severity of food insecurity in the previous year, the difference in odds was 45%. Conclusions: The results are consistent with, or somewhat higher than, the estimates from the strongest previous research designs and suggest that the ameliorative effect of SNAP on very low food security is in the range of 20-50%. C1 ERS, USDA, Washington, DC 20036 USA. RP Nord, M (reprint author), ERS, USDA, 1800M St NW,Room 2180, Washington, DC 20036 USA. EM marknord@ers.usda.gov NR 22 TC 13 Z9 13 U1 1 U2 18 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD MAY PY 2012 VL 15 IS 5 BP 811 EP 817 DI 10.1017/S1368980011002709 PG 7 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA 919XP UT WOS:000302365800008 PM 22015063 ER PT J AU Cheng, WH Holmstrom, A Li, XD Wu, RTY Zeng, HW Xiao, ZG AF Cheng, Wen-Hsing Holmstrom, Alexandra Li, Xiangdong Wu, Ryan T. Y. Zeng, Huawei Xiao, Zhengguo TI Effect of Dietary Selenium and Cancer Cell Xenograft on Peripheral T and B Lymphocytes in Adult Nude Mice SO BIOLOGICAL TRACE ELEMENT RESEARCH LA English DT Article DE Selenium; Cancer; T cell; B cell ID GROWTH-FACTOR-BETA; PROSTATE-CANCER; IMMUNE-SYSTEM; PREVENTION; SELENOMETHIONINE; SELENOPROTEINS; MECHANISM; APOPTOSIS AB Selenium (Se) is known to regulate tumorigenesis and immunity at the nutritional and supranutritional levels. Because the immune system provides critical defenses against cancer and the athymic, immune-deficient NU/J nude mice are known to gradually develop CD8(+) and CD4(+) T cells, we investigated whether B and T cell maturation could be modulated by dietary Se and by tumorigenesis in nude mice. Fifteen homozygous nude mice were fed a Se-deficient, Torula yeast basal diet alone (Se-) or supplemented with 0.15 (Se+) or 1.0 (Se++) mg Se/kg (as Na2SeO4) for 6 months, followed by a 7-week time course of PC-3 prostate cancer cell xenograft (2 x 10(6) cells/site, 2 sites/mouse). Here, we show that peripheral B cell levels decreased in nude mice fed the Se -aEuro parts per thousand or Se++ diet and the CD4(+) T cell levels increased in mice fed the Se++ diet. During the PC-3 cell tumorigenesis, dietary Se status did not affect peripheral CD4(+) or CD8(+) T cells in nude mice whereas mice fed with the Se++ diet appeared to exhibit greater peripheral CD25(+)CD4(+) T cells on day 9. Dietary Se status did not affect spleen weight in nude mice 7 weeks after the xenograft. Spleen weight was associated with frequency of peripheral CD4(+), but not CD8(+) T cells. Taken together, dietary Se at the nutritional and supranutritional levels regulates peripheral B and T cells in adult nude mice before and after xenograft with PC-3 prostate cancer cells. C1 [Cheng, Wen-Hsing; Holmstrom, Alexandra; Wu, Ryan T. Y.] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Li, Xiangdong; Xiao, Zhengguo] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Zeng, Huawei] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND 58202 USA. RP Cheng, WH (reprint author), Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. EM whcheng@umd.edu; xiao0028@umd.edu NR 29 TC 4 Z9 4 U1 1 U2 7 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0163-4984 J9 BIOL TRACE ELEM RES JI Biol. Trace Elem. Res. PD MAY PY 2012 VL 146 IS 2 BP 230 EP 235 DI 10.1007/s12011-011-9235-2 PG 6 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA 915UJ UT WOS:000302052600014 PM 22020772 ER PT J AU Pandey, MK Monyo, E Ozias-Akins, P Liang, XQ Guimaraes, P Nigam, SN Upadhyaya, HD Janila, P Zhang, XY Guo, BZ Cook, DR Bertioli, DJ Michelmore, R Varshney, RK AF Pandey, Manish K. Monyo, Emmanuel Ozias-Akins, Peggy Liang, Xuanquiang Guimaraes, Patricia Nigam, Shyam N. Upadhyaya, Hari D. Janila, Pasupuleti Zhang, Xinyou Guo, Baozhu Cook, Douglas R. Bertioli, David J. Michelmore, Richard Varshney, Rajeev K. TI Advances in Arachis genomics for peanut improvement SO BIOTECHNOLOGY ADVANCES LA English DT Review DE Groundnut; Molecular markers; Molecular breeding; Genomic resources; Genetic improvement ID MARKER-ASSISTED SELECTION; ASPERGILLUS-PARASITICUS INFECTION; EXPRESSED SEQUENCE TAGS; GENETIC-LINKAGE MAP; HYPOGAEA L.; CULTIVATED PEANUT; MICROSATELLITE MARKERS; NEMATODE RESISTANCE; GERMPLASM COLLECTION; CORE COLLECTION AB Peanut genomics is very challenging due to its inherent problem of genetic architecture. Blockage of gene flow from diploid wild relatives to the tetraploid; cultivated peanut, recent polyploidization combined with self pollination, and the narrow genetic base of the primary genepool have resulted in low genetic diversity that has remained a major bottleneck for genetic improvement of peanut. Harnessing the rich source of wild relatives has been negligible due to differences in ploidy level as well as genetic drag and undesirable alleles for low yield. Lack of appropriate genomic resources has severely hampered molecular breeding activities, and this crop remains among the less-studied crops. The last five years, however, have witnessed accelerated development of genomic resources such as development of molecular markers, genetic and physical maps, generation of expressed sequenced tags (ESTs), development of mutant resources, and functional genomics platforms that facilitate the identification of QTLs and discovery of genes associated with tolerance/resistance to abiotic and biotic stresses and agronomic traits. Molecular breeding has been initiated for several traits for development of superior genotypes. The genome or at least gene space sequence is expected to be available in near future and this will further accelerate use of biotechnological approaches for peanut improvement. (C) 2011 Elsevier Inc. All rights reserved. C1 [Pandey, Manish K.; Nigam, Shyam N.; Upadhyaya, Hari D.; Janila, Pasupuleti; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. [Monyo, Emmanuel] Int Crops Res Inst Semi Arid Trop, Lilongwe 1096, Malawi. [Ozias-Akins, Peggy] Univ Georgia, Athens, GA 30602 USA. [Liang, Xuanquiang] Guandong Acad Agr Sci, Crops Res Inst, Guangzhou 510640, Guangdong, Peoples R China. [Guimaraes, Patricia] EMBRAPA, Natl Ctr Genet Resources & Biotechnol, BR-70770900 Brasilia, DF, Brazil. [Zhang, Xinyou] Henan Acad Agr Sci, Zhengzhou 450002, Peoples R China. [Guo, Baozhu] USDA ARS, Crop Protect & Management Unit, Tifton, GA 31793 USA. [Cook, Douglas R.; Michelmore, Richard] Univ Calif Davis, Davis, CA 95616 USA. [Bertioli, David J.] Univ Brasilia, BR-70910900 Brasilia, DF, Brazil. [Varshney, Rajeev K.] CIMMYT, CGIAR Generat Challenge Programme GCP, Mexico City 06600, DF, Mexico. [Varshney, Rajeev K.] Univ Western Australia, Crawley, WA 6009, Australia. RP Varshney, RK (reprint author), Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. EM r.k.varshney@cgiar.org RI Updhayaya, Hari/C-4858-2014; Varshney, Rajeev/C-5295-2014 OI Updhayaya, Hari/0000-0002-5166-6844; Varshney, Rajeev/0000-0002-4562-9131 FU Indian Council of Agriculture Research (ICAR), New Delhi, India; CGIAR FX Financial support received from National Fund of the Basic and Strategic Research in Agriculture (NFBSRA) of the Indian Council of Agriculture Research (ICAR), New Delhi, India and Generation Challenge Programme (http://www.generationcp.org) of CGIAR is gratefully acknowledged. NR 123 TC 59 Z9 65 U1 1 U2 47 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0734-9750 J9 BIOTECHNOL ADV JI Biotechnol. Adv. PD MAY-JUN PY 2012 VL 30 IS 3 BP 639 EP 651 DI 10.1016/j.biotechadv.2011.11.001 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 913TY UT WOS:000301901500013 PM 22094114 ER PT J AU Dercon, G Mabit, L Hancock, G Nguyen, ML Dornhofer, R Bacchi, OOS Benmansour, M Bernard, C Froehlich, W Golosov, VN Haciyakupoglu, S Hai, PS Klik, A Li, Y Lobb, DA Onda, Y Popa, N Rafiq, M Ritchie, JC Schuller, P Shakhashiro, A Wallbrink, P Walling, DE Zapata, F Zhang, X AF Dercon, G. Mabit, L. Hancock, G. Nguyen, M. L. Dornhofer, R. Bacchi, O. O. S. Benmansour, M. Bernard, C. Froehlich, W. Golosov, V. N. Haciyakupoglu, S. Hai, P. S. Klik, A. Li, Y. Lobb, D. A. Onda, Y. Popa, N. Rafiq, M. Ritchie, J. C. Schuller, P. Shakhashiro, A. Wallbrink, P. Walling, D. E. Zapata, F. Zhang, X. TI Fallout radionuclide-based techniques for assessing the impact of soil conservation measures on erosion control and soil quality: an overview of the main lessons learnt under an FAO/IAEA Coordinated Research Project SO JOURNAL OF ENVIRONMENTAL RADIOACTIVITY LA English DT Review DE Caesium-137; Lead-210; Beryllium-7; FRN; Land degradation; Agricultural landscapes ID SICHUAN HILLY BASIN; AMERICAN GREAT-PLAINS; SPATIAL-DISTRIBUTION; ENVIRONMENTAL RADIONUCLIDES; CS-137 MEASUREMENTS; ANDES REGION; CESIUM-137 MEASUREMENTS; PB-210(EX) MEASUREMENTS; DIFFERENT LANDSCAPES; SEDIMENT SOURCES AB This paper summarizes key findings and identifies the main lessons learnt from a 5-year (2002-2008) coordinated research project (CRP) on "Assessing the effectiveness of soil conservation measures for sustainable watershed management and crop production using fallout radionuclides" (D1.50.08), organized and funded by the International Atomic Energy Agency through the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. The project brought together nineteen participants, from Australia, Austria, Brazil, Canada, Chile, China, japan, Morocco, Pakistan, Poland, Romania, Russian Federation, Turkey, United Kingdom, United States of America and Vietnam, involved in the use of nuclear techniques and, more particularly, fallout radionuclides (FRN) to assess the relative impacts of different soil conservation measures on soil erosion and land productivity. The overall objective of the CRP was to develop improved land use and management strategies for sustainable watershed management through effective soil erosion control practices, by the use of Cs-137 (half-life of 30.2 years), Pb-210(ex). no (half-life of 22.3 years) and Be-7 (half-life of 53.4 days) for measuring soil erosion over several spatial and temporal scales. The environmental conditions under which the different research teams applied the tools based on the use of fallout radionuclides varied considerably - a variety of climates, soils, topographies and land uses. Nevertheless, the achievements of the CRP, as reflected in this overview paper, demonstrate that fallout radionuclide-based techniques are powerful tools to assess soil erosion/deposition at several spatial and temporal scales in a wide range of environments, and offer potential to monitor soil quality. The success of the CRP has stimulated an interest in many IAEA Member States in the use of these methodologies to identify factors and practices that can enhance sustainable agriculture and minimize land degradation. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Dercon, G.; Mabit, L.; Nguyen, M. L.; Dornhofer, R.; Bernard, C.; Zapata, F.] IAEA, Dept Nucl Sci & Applicat, Joint FAO IAEA Div Nucl Tech Food & Agr, A-1400 Vienna, Austria. [Hancock, G.; Wallbrink, P.] CSIRO, Div Land & Water, Canberra, ACT, Australia. [Bacchi, O. O. S.] Univ Sao Paulo, Ctr Nucl Energy Agr, Piracicaba, Brazil. [Benmansour, M.] Ctr Natl Energie Sci & Tech Nucl, Rabat, Morocco. [Froehlich, W.] Polish Acad Sci, Inst Geog & Spatial Org, Homerka Lab Fluvial Proc, Nawojowa, Malopolska, Poland. [Golosov, V. N.] Moscow MV Lomonosov State Univ, Fac Geog, Lab Soil Eros & Fluvial Proc, Moscow, Russia. [Golosov, V. N.] Kazan Fed Univ, Kazan, Russia. [Haciyakupoglu, S.] Istanbul Tech Univ, Energy Inst, TR-80626 Istanbul, Turkey. [Hai, P. S.] Dalat Nucl Res Inst, Ctr Environm Res & Monitoring, Da Lat, Vietnam. [Klik, A.] Univ Nat Resources & Appl Life Sci, Inst Hydraul & Rural Water Management, Vienna, Austria. [Li, Y.] Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100193, Peoples R China. [Lobb, D. A.] Univ Manitoba, Winnipeg, MB, Canada. [Onda, Y.] Univ Tsukuba, Sch Life & Environm Sci, Lab Integrat Environm Sci, Tsukuba, Ibaraki, Japan. [Popa, N.] Res & Dev Ctr Soil Eros Control, Perieni, Romania. [Rafiq, M.] Pakistan Inst Nucl Sci & Technol, Islamabad, Pakistan. [Ritchie, J. C.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Schuller, P.] Univ Austral Chile, Fac Ciencias, Inst Ciencas Quim, Valdivia, Chile. [Zhang, X.] Chinese Acad Sci, Inst Mt Hazards & Environm, Chengdu, Sichuan, Peoples R China. RP Dercon, G (reprint author), IAEA, Dept Nucl Sci & Applicat, Joint FAO IAEA Div Nucl Tech Food & Agr, Wagramerstr 5, A-1400 Vienna, Austria. EM g.dercon@iaea.org RI Wallbrink, Peter/A-2794-2011; Onda, Yuichi/N-6527-2014; Wallbrink, Peter/A-7107-2017 OI Onda, Yuichi/0000-0001-8753-0378; FU IAEA [E1.02] FX The authors acknowledge the IAEA 2002-2008 funding of the CRP on "Assessing the effectiveness of soil conservation measures for sustainable watershed management and crop production using fallout radionuclides" (D1.50.08) provided under the IAEA Project E1.02 "Development of soil management and conservation practices for sustainable crop production and environmental protection". NR 83 TC 11 Z9 11 U1 3 U2 78 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0265-931X J9 J ENVIRON RADIOACTIV JI J. Environ. Radioact. PD MAY PY 2012 VL 107 BP 78 EP 85 DI 10.1016/j.jenvrad.2012.01.008 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 913TH UT WOS:000301899800008 PM 22336567 ER PT J AU Hunter, WJ Shaner, DL AF Hunter, William J. Shaner, Dale L. TI Removing Hexazinone from Groundwater with Microbial Bioreactors SO CURRENT MICROBIOLOGY LA English DT Article ID DEGRADATION CAPACITY; ATRAZINE DEGRADATION; HERBICIDE ATRAZINE; BACTERIAL ISOLATE; NITROGEN; MINERALIZATION; DENITRIFICATION; SOILS; ACID; AVAILABILITY AB Hexazinone, a triazine herbicide that is often detected as a ground and surface water contaminant, inhibits electron transport in photosynthetic organisms and is toxic to primary producers that serve as the base of the food chain. This laboratory study evaluated the ability of two types of microbial reactors, i.e., a vegetable oil-based nitrogen-limiting biobarrier and an aerobic slow sand filter, as methods for removing hexazinone from simulated groundwater. The N-limiting biobarriers degraded hexazinone, but did so with a 52 week incubation period and a removal efficiency that varied greatly among replicates, with one biobarrier showing a removal efficiency of similar to 95% and the other an efficiency of similar to 50%. More consistent degradation was obtained with the aerobic sand biobarriers. Four aerobic biobarriers were evaluated and all behaved in a similar manner degrading hexazinone with removal efficiencies of similar to 97%; challenging two of the aerobic biobarriers with large amounts of influent hexazinone showed that these barriers are capable of efficiently remediating large amounts (> 100 mg L-1) of hexazinone at high efficiency. The remediation process was due to biological degradation rather than abiotic processes. The long lag phase observed in both types of reactors suggests that an acclimation process, where microorganisms capable of degrading hexazinone increased in numbers, was required. Also, the isolation of bacteria that show a positive growth response to the presence of hexazinone in their growth media suggests biological degradation. C1 [Hunter, William J.; Shaner, Dale L.] USDA ARS, Ft Collins, CO 80526 USA. RP Hunter, WJ (reprint author), USDA ARS, 2150-D Ctr Ave, Ft Collins, CO 80526 USA. EM william.hunter@ars.usda.gov; dale.shaner@ars.usda.gov OI Shaner, Dale/0000-0003-4293-6133 NR 39 TC 2 Z9 2 U1 0 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 J9 CURR MICROBIOL JI Curr. Microbiol. PD MAY PY 2012 VL 64 IS 5 BP 405 EP 411 DI 10.1007/s00284-012-0086-7 PG 7 WC Microbiology SC Microbiology GA 912ZL UT WOS:000301841000001 PM 22302451 ER PT J AU Setamou, M Sanchez, A Patt, JM Nelson, SD Jifon, J Louzada, ES AF Setamou, Mamoudou Sanchez, Adrian Patt, Joseph M. Nelson, Shad D. Jifon, John Louzada, Eliezer S. TI Diurnal Patterns of Flight Activity and Effects of Light on Host Finding Behavior of the Asian Citrus Psyllid SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Diaphorina citri; light; phototaxis; circadian rhythm-host plant selection; Huanglongbing ID KUWAYAMA HEMIPTERA PSYLLIDAE; STICKY TRAPS; DIAPHORINA; INTENSITY; VOLATILES; HOMOPTERA; RESPONSES; SELECTION; DISEASE; FLORIDA AB The Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae), is an invasive pest of citrus in the United States. The psyllid feeds and reproduces primarily on new flush growth of citrus and other rutaceous plants. Because it vectors the bacterial causal agents of the deadly citrus greening disease, D. citri is potentially a pest of economic importance in all citrus growing areas where it occurs together with the disease. We investigated the diurnal patterns of its flight activity in the field and the effects of light on its host selection and egg laying behaviors. The numbers of adult psyllids caught on yellow sticky traps were 3 to 4-fold higher during daytime than nighttime. Daytime flight activity of D. citri adults also varied with time of the day with peak catches occurring at midday from 1200 to 1500 h. Illumination of the traps at night increased their attractiveness to adult psyllids by 5-fold. Similarly, light significantly increased plant colonization by adults and female egg deposition on potted plants in the laboratory. These results showed that the flight activity and host selection behavior of adult psyllids are regulated by light and circadian rhythms. Thus, adult psyllids utilize light as visual cues in their host-plant selection process. C1 [Setamou, Mamoudou; Sanchez, Adrian; Nelson, Shad D.; Louzada, Eliezer S.] Texas A&M Univ Kingsville, Citrus Ctr, Weslaco, TX 78596 USA. [Patt, Joseph M.] ARS, USDA, KSARC, Weslaco, TX 78596 USA. [Jifon, John] Texas AgriLife Res, Weslaco, TX 78596 USA. RP Setamou, M (reprint author), Texas A&M Univ Kingsville, Citrus Ctr, 312 N Int Blvd, Weslaco, TX 78596 USA. EM mamoudou.setamou@tamuk.edu FU Texas Citrus Producers Board FX We thank Robert R. Saldana and Daniel Davila for their assistance with data collection. An earlier version of the manuscript was improved by comments from Drs. J. da Graca (Texas A&M University-Kingsville Citrus Center) and D. Thomas (USDA-ARS, Weslaco). Partial funding for the study was provided by the Texas Citrus Producers Board. NR 26 TC 12 Z9 13 U1 3 U2 25 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 J9 J INSECT BEHAV JI J. Insect Behav. PD MAY PY 2012 VL 25 IS 3 BP 264 EP 276 DI 10.1007/s10905-011-9295-3 PG 13 WC Entomology SC Entomology GA 912II UT WOS:000301789900005 ER PT J AU Dungan, RS AF Dungan, Robert S. TI Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation SO ENVIRONMENT INTERNATIONAL LA English DT Article DE Aerosol; Dairy; Manure; PCR; Spray irrigation; Wastewater ID 16S RIBOSOMAL-RNA; ESCHERICHIA-COLI; FEEDING OPERATIONS; AIRBORNE BACTERIA; SWINE; BIOAEROSOLS; SURVIVAL; FRESH; TRANSMISSION; SALMONELLA AB Animal manures are known to harbor a variety of zoonotic pathogens, which are suspected of being transported off-site as aerosols from confined feeding operations. In this study, aerosols were collected using a high-volume sampler downwind from a 10,000 cow open-freestall dairy and nearby fields being sprinkler irrigated with wastewater. DNA extracts were prepared from the aerosol samples, then a region of the 16S ribosomal RNA gene was sequenced for bacterial identification and phylogenetic classification. At the dairy and irrigation sites, Proteobacteria (alpha-, beta-, and gamma-subdivisions) was the most abundant phylum, representing 78% and 69% of all sequences, respectively, while Actinobacteria, Bacteroidetes and Firmicutes represented only 10% or less of the sequences. Of the 191 clones sequenced from the dairy aerosol samples, 6 sequences were found to be homologous with uncultured bacteria from cow milk, rumen. and fecal samples. However, none of the sequence matches was affiliated with bacteria known to be pathogenic to otherwise healthy humans. Although our results do suggest a high diversity among the aerosolized bacteria, the sampling strategy employed in this study may not account for the variable nature of bioaerosol emissions. Published by Elsevier Ltd. C1 USDA ARS, NW Irrigat & Soils Res Lab NWIRSL, Kimberly, ID 83341 USA. RP Dungan, RS (reprint author), USDA ARS, NW Irrigat & Soils Res Lab NWIRSL, Kimberly, ID 83341 USA. EM robert.dungan@ars.usda.gov RI perumal, murugiah/D-1565-2012 NR 39 TC 11 Z9 11 U1 5 U2 23 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0160-4120 J9 ENVIRON INT JI Environ. Int. PD MAY PY 2012 VL 41 BP 8 EP 14 DI 10.1016/j.envint.2011.12.004 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA 910YO UT WOS:000301684700002 PM 22341661 ER PT J AU Zhu, XL Gao, F Liu, DS Chen, J AF Zhu, Xiaolin Gao, Feng Liu, Desheng Chen, Jin TI A Modified Neighborhood Similar Pixel Interpolator Approach for Removing Thick Clouds in Landsat Images SO IEEE GEOSCIENCE AND REMOTE SENSING LETTERS LA English DT Article DE Cloud removal; image processing; image restoration; Landsat AB Thick-cloud contamination is a common problem in Landsat images, which limits their utilities in various land surface studies. This letter presents a new method for removing thick clouds based on a modified neighborhood similar pixel interpolator (NSPI) approach that was originally developed for filling gaps due to the Landsat ETM+ Scan Line Corrector (SLC)-off problem. The performance of the proposed method was evaluated with both simulated and real cloudy images and compared with that of a contextual multiple linear prediction (CMLP) method. The results show that the modified NSPI approach can greatly reduce the edge effects by CMLP. The reflectance restored by the modified NSPI approach is more accurate than that by CMLP, especially when the cloud-free auxiliary and cloudy images are acquired from different seasons and have different spectral characteristics. C1 [Zhu, Xiaolin; Chen, Jin] Beijing Normal Univ, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China. [Zhu, Xiaolin; Liu, Desheng] Ohio State Univ, Dept Geog, Columbus, OH 43210 USA. [Liu, Desheng] Ohio State Univ, Dept Stat, Columbus, OH 43210 USA. [Gao, Feng] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Zhu, XL (reprint author), Ohio State Univ, Dept Geog, Columbus, OH 43210 USA. EM liu.738@osu.edu RI Chen, Jin/A-6417-2011; Zhu, Xiaolin/K-8175-2012; Chen, Jin/I-7666-2016; Chen, Lijun/Q-5012-2016; Liu, Desheng/A-9356-2011 OI Chen, Jin/0000-0002-6497-4141; Zhu, Xiaolin/0000-0001-6967-786X; FU Project 863 of China [2009AA12004]; National Science Foundation [1010314]; State Key Laboratory of Earth Surface Processes and Resource Ecology; International S&T Cooperation Program [2010DFB10030] FX This work was supported in part by the Project 863 of China under Grant 2009AA12004, by the National Science Foundation under Award 1010314, by the State Key Laboratory of Earth Surface Processes and Resource Ecology, and by the International S&T Cooperation Program under Grant 2010DFB10030. NR 13 TC 16 Z9 21 U1 3 U2 27 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1545-598X J9 IEEE GEOSCI REMOTE S JI IEEE Geosci. Remote Sens. Lett. PD MAY PY 2012 VL 9 IS 3 BP 521 EP 525 DI 10.1109/LGRS.2011.2173290 PG 5 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA 904YV UT WOS:000301236900041 ER PT J AU Wang, ZJ Zhu, JY Zalesny, RS Chen, KF AF Wang, Z. J. Zhu, J. Y. Zalesny, Ronald S., Jr. Chen, K. F. TI Ethanol production from poplar wood through enzymatic saccharification and fermentation by dilute acid and SPORL pretreatments SO FUEL LA English DT Article DE Aspen and poplar; Enzymatic hydrolysis and fermentation; Cellulosic ethanol; Pretreatment; Recalcitrance ID LODGEPOLE PINE; OVERCOME RECALCITRANCE; SULFITE PRETREATMENT; ENERGY EFFICIENCY; BIOMASS; LIGNOCELLULOSE; CELLULOSE; CROPS AB Dilute acid (DA) and Sulfite Pretreatment to Overcome Recalcitrance of Lignocelluloses (SPORL) pretreatments were directly applied to wood chips of four poplar wood samples of different genotypes (hereafter referred to as poplars; Populus tremuloides Michx. 'native aspen collection'; Populus deltoides Bartr. ex Marsh x Populus nigra L. 'NE222' and 'DN5'; P. nigra x Populus maximowiczii A. Henry 'NM6') to evaluate their bioconversion potential. Plant biomass recalcitrance (PBR) was defined to quantitatively determine the recalcitrance of the poplars. Using DA pretreatment, NM6 produced the lowest bioconversion efficiency with a total monomeric sugar yield of 18% theoretical and an ethanol yield of 0.07 L kg (1) of wood compared with an aspen sugar yield of 47% theoretical and an ethanol yield of 0.17 L kg (1) of wood. Similar comparisons following SPORL pretreatment were 43% versus 55% and 0.11 versus 0.20 L kg (1) of wood for NM6 and aspen, respectively. Bioconversion performance of NE222 and DN5 fell between that of aspen and NM6. While substrate lignin content and lignin removal by pretreatments did not affect substrate enzymatic digestibility, the wood lignin content was found to negatively affect xylan or hemicellulose removal using both DA and SPORL pretreatments. The ability of lignin protecting hemicellulose removal dictates PBR through affecting disk milling energy for size reduction of pretreated wood chips, substrate enzymatic digestibility. The SPORL pretreatment not only improved sugar and ethanol yields over DA for all four poplars, but also better dealt with the differences among them, suggesting better tolerance to feedstock variability. Published by Elsevier Ltd. C1 [Wang, Z. J.; Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. [Wang, Z. J.; Chen, K. F.] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou, Guangdong, Peoples R China. [Zalesny, Ronald S., Jr.] US Forest Serv, USDA, No Res Stn, Rhinelander, WI USA. RP Zhu, JY (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI USA. EM jzhu@fs.fed.us FU US Forest Service (USFS); USDA Small Business Innovative Research (SBIR) [2010-33610-21589]; Chinese Scholarship Council (CSC) FX This work was sponsored by the US Forest Service (USFS) through the Program of Woody Biomass, Bioenergy, and Bioproducts (WBBB, 2011) and a USDA Small Business Innovative Research (SBIR) Phase II project (Contract Number: 2010-33610-21589) to BioPulping International. We acknowledge Diane Dietrich of US Forest Service, Forest Products Laboratory (USFS-FPL) for carrying out many careful analyses of ethanol in fermentation broth samples, Fred Matt (USFS-FPL) for carbohydrate composition analysis of the solid substrates, and Dr. Thomas Jeffries of FPL and his staff for providing the yeast for fermentation. The Chinese Scholarship Council (CSC) and the USDA SBIR Phase II project provided financial support to Wang for his visiting appointment at USFS-FPL. NR 31 TC 50 Z9 54 U1 1 U2 55 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0016-2361 J9 FUEL JI Fuel PD MAY PY 2012 VL 95 IS 1 BP 606 EP 614 DI 10.1016/j.fuel.2011.12.032 PG 9 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 897BM UT WOS:000300615900079 ER PT J AU Xie, ZH Huang, HQ Zhao, Y Shi, HM Wang, SK Wang, TTY Chen, P Yu, LL AF Xie, Zhuohong Huang, Haiqiu Zhao, Yang Shi, Haiming Wang, Shaoke Wang, Thomas T. Y. Chen, Pei Yu, Liangli (Lucy) TI Chemical composition and anti-proliferative and anti-inflammatory effects of the leaf and whole-plant samples of diploid and tetraploid Gynostemma pentaphyllum (Thunb.) Makino SO FOOD CHEMISTRY LA English DT Article DE Tetraploid; Gynostemma pentaphyllum; Antioxidant; Anti-proliferation; Anti-inflammation ID PROSTATE-CANCER CELLS; INDUCED G0/G1 ARREST; ANTIOXIDANT PROPERTIES; DEPENDENT PATHWAYS; LUNG-CANCER; FLAVONOIDS; APOPTOSIS; RECEPTOR; ASSAY; MICE AB Leaf and whole-plant samples of the diploid and tetraploid Gynostemma pentaphyllum (GP) were investigated and compared for their chemical compositions, and their potential anti-proliferative and anti-inflammatory effects. The highest levels of total flavonoids and phenolics were observed in the diploid leaf botanical (2L3) at 36.84 mg rutin equiv/g and 41.15 mg gallic acid equiv/g, respectively. The diploid leaf sample (2L2) had the highest amount of rutin and quercetin contents of 77.7 mu mol quercetin equiv/g. The tetraploid whole-plant botanical (4L3) had the highest total saponin content of 227.1 mg gypenoside equiv/g. Extracts from all tested GP samples showed time- and dose-dependent antiproliferative effects in HT-29 cells, and the diploid leaf samples had the overall highest inhibitory activity. These extracts had different order of antiproliferative properties in the LNCaP cells, suggesting the potential selective inhibition of GP extracts against different types of cancer cells and the effect of the cell model in screening and evaluation of antiproliferative components. In addition, the diploid leaf extracts showed the strongest inhibitory effects on the expression of TNF-alpha, IL-6 and COX-2 mRNA at final concentrations of 0.2 and 1 mg botanical equiv/ml media. The results from this study will be used to develop new nutraceutical products from G. pentaphyllum. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Xie, Zhuohong; Huang, Haiqiu; Zhao, Yang; Yu, Liangli (Lucy)] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Shi, Haiming; Yu, Liangli (Lucy)] Shanghai Jiao Tong Univ, Sch Agr & Biol, Key Lab Urban Agr S, Inst Food & Nutraceut Sci, Shanghai 200240, Peoples R China. [Wang, Shaoke] Asian Citrus Holdings Ltd, Hong Kong, Hong Kong, Peoples R China. [Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Chen, Pei] ARS, Food Composit Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Yu, LL (reprint author), Univ Maryland, Dept Nutr & Food Sci, 0112 Skinner Bldg, College Pk, MD 20742 USA. EM lyu5@umd.edu RI Xie, Zhuohong/E-4543-2012; Huang, Haiqiu/J-8856-2012; Xie, Zhuohong/P-3599-2014; shi, haiming/D-2806-2015; ZHAO, YANG/O-7920-2014 OI Xie, Zhuohong/0000-0001-8009-3523; FU Joint Institute for Food Safety and Applied Nutrition (JIFSAN); Maryland Grain Producers Utilization Board (MGPUB); Maryland TEDCO FX This research was partially supported by a grant from the Joint Institute for Food Safety and Applied Nutrition (JIFSAN), a research gift from the Asian Citrus Holdings Limited (Hong Kong), a grant from the Maryland Grain Producers Utilization Board (MGPUB), and a grant from Maryland TEDCO. NR 30 TC 16 Z9 16 U1 3 U2 42 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAY 1 PY 2012 VL 132 IS 1 BP 125 EP 133 DI 10.1016/j.foodchem.2011.10.043 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 894ZD UT WOS:000300465100016 PM 26434271 ER PT J AU Cabanillas, B Maleki, SJ Rodriguez, J Burbano, C Muzquiz, M Jimenez, MA Pedrosa, MM Cuadrado, C Crespo, JF AF Cabanillas, Beatriz Maleki, Soheila J. Rodriguez, Julia Burbano, Carmen Muzquiz, Mercedes Aranzazu Jimenez, Maria Pedrosa, Mercedes M. Cuadrado, Carmen Crespo, Jesus F. TI Heat and pressure treatments effects on peanut allergenicity SO FOOD CHEMISTRY LA English DT Article DE Peanut allergy; Thermal-processing methods; Autoclave treatment ID IGE-BINDING; FOOD ALLERGENS; PROTEINS; EPITOPES; LENTIL AB Peanut allergy is recognized as one of the most severe food allergies. The aim of this study was to investigate the changes in IgE binding capacity of peanut proteins produced by thermal-processing methods, including autoclaving. Immunoreactivity to raw and thermally processed peanut extracts was evaluated by IgE immunoblot and skin prick test in patients with clinical allergy to peanut. Roasted peanut and autoclaved roasted peanut were selected for IgE ELISA experiments with individual sera, immunoblot experiments with antibodies against peanut allergens (Ara h 1, Ara h 2 and Ara h 3), digestion experiments, and circular dichroism spectroscopy. In vitro and in vivo experiments showed IgE immunoreactivity of roasted peanut proteins decreased significantly at extreme conditions of autoclaving. Circular dichroism experiments showed unfolding of proteins in autoclave treated samples, which makes them more susceptible to digestion. Autoclaving at 2.56 atm, for 30 min, produces a significant decrease of IgE-binding capacity of peanut allergens. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Cabanillas, Beatriz; Rodriguez, Julia; Aranzazu Jimenez, Maria; Crespo, Jesus F.] Hosp Univ 12 Octubre, Serv Alergia, Inst Invest Hosp, Madrid 28041, Spain. [Maleki, Soheila J.] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. [Burbano, Carmen; Muzquiz, Mercedes; Pedrosa, Mercedes M.; Cuadrado, Carmen] SGIT INIA, Dept Tecnol Alimentos, Madrid 28040, Spain. RP Cabanillas, B (reprint author), Hosp Univ 12 Octubre, Serv Alergia, Inst Invest Hosp, Ave Cordoba S-N, Madrid 28041, Spain. EM bcabanillas.hdoc@salud.madrid.org RI Burbano, Carmen/C-4725-2013; CUADRADO, CARMEN/C-5504-2013; Pedrosa, Mercedes/O-2353-2014; Rodriguez Herrera, Juan Jose/I-3210-2015; Rodriguez, Maria/P-1090-2014; OI CUADRADO, CARMEN/0000-0003-2609-1900; Pedrosa, Mercedes/0000-0002-8313-9790; Rodriguez, Maria/0000-0002-1599-8227; Cabanillas, Beatriz/0000-0002-5351-8140 FU Ministerio de Educacion y Ciencia [AGL2004-07971-C02, AGL2008-03453-C02]; Instituto Carlos III Ministerio de Ciencia e Innovacion [FI07/00286] FX This study was supported by Grant No. AGL2004-07971-C02, Grant AGL2008-03453-C02, Ministerio de Educacion y Ciencia and Grant No. FI07/00286 Instituto Carlos III Ministerio de Ciencia e Innovacion. NR 28 TC 20 Z9 23 U1 5 U2 50 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAY 1 PY 2012 VL 132 IS 1 BP 360 EP 366 DI 10.1016/j.foodchem.2011.10.093 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 894ZD UT WOS:000300465100047 PM 26434302 ER PT J AU Jin, P Wang, SY Gao, HY Chen, HJ Zheng, YH Wang, CY AF Jin, Peng Wang, Shiow Y. Gao, Haiyan Chen, Hangjun Zheng, Yonghua Wang, Chien Y. TI Effect of cultural system and essential oil treatment on antioxidant capacity in raspberries SO FOOD CHEMISTRY LA English DT Article DE Raspberries; Antioxidant enzyme; Antioxidant capacity; Cultural system; Essential oil ID NATURAL VOLATILE COMPOUNDS; FLAVONOID CONTENT; STRAWBERRY FRUIT; PHENOLICS; QUALITY; PEROXIDASE; ASSAY; FUNGITOXICITY; BLUEBERRIES; METABOLISM AB The effects of cultural system and essential oil treatment on antioxidant capacities in raspberries were evaluated. Raspberries were hand-harvested from organic and conventional farms in Maryland, USA, and were treated with essential oil including carvacrol, anethole, cinnamic acid, perillaldehyde, cinnamaldehyde, and linalool. Results from this study showed that raspberries grown from organic culture exhibited higher value of antioxidant capacities and individual flavonoids contents. Moreover, the organic culture also enhanced the activities of antioxidant enzymes. In addition, essential oil treatments promoted the antioxidant enzymes activities and antioxidant capacities of raspberries, and the most effective compound was perillaldehyde. In conclusion, raspberries produced from organic culture contained significantly higher antioxidant capacities than those produce from conventional culture. Postharvest essential oil treatments have positive effect on enhancing antioxidant capacities in raspberries from both organic and conventional cultures. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Jin, Peng; Zheng, Yonghua] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Shiow Y.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. [Gao, Haiyan; Chen, Hangjun] Zhejiang Acad Agr Sci, Food Sci Div, Hangzhou, Zhejiang, Peoples R China. [Jin, Peng; Wang, Chien Y.] ARS, Food Qual Lab, USDA, Beltsville, MD 20705 USA. RP Zheng, YH (reprint author), Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China. EM zhengyh@njau.edu.cn NR 40 TC 20 Z9 21 U1 5 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD MAY 1 PY 2012 VL 132 IS 1 BP 399 EP 405 DI 10.1016/j.foodchem.2011.11.011 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 894ZD UT WOS:000300465100052 PM 26434307 ER PT J AU Wise, LN Ueti, MW Kappmeyer, LS Hines, MT White, SN Davis, W Knowles, DP AF Wise, L. Nicki Ueti, Massaro W. Kappmeyer, Lowell S. Hines, Melissa T. White, Stephen N. Davis, Wendell Knowles, Donald P. TI In vitro activity of ponazuril against Theileria equi SO VETERINARY PARASITOLOGY LA English DT Article DE Piroplasmosis; Ponazuril; Therapy ID PIROPLASMOSIS BABESIA-EQUI; IMIDOCARB; PARASITES; CULTURE; EFFICACY; DONKEYS; HORSES AB The equid hemoprotozoan parasite Theileria equi is endemic in most regions worldwide. Infection of horses is a cause of significant economic loss due to costs associated with disease and restriction of trade with non-endemic nations. The ability of certain drugs such as imidocarb dipropionate to eliminate persistent T. equi infection and transmission risk is controversial. The anti-protozoal agent ponazuril has been used successfully to treat equine Sarcosystis neurona and Toxoplasma gondii. The hypothesis that ponazuril inhibits replication of T. equi in vitro was tested. T. equi infected equine erythrocyte cultures were treated with ponazuril at multiple concentrations. Cessation of parasite replication was observed over a 5-day period and the degree of inhibition was variable between drug concentrations. Ponazuril inhibited T. equi in erythrocyte culture at all concentrations tested but parasite elimination required at least 500 mu g/mL The high dose of ponazuril required for in vitro inhibition likely limits its ability to control or clear T. equi infection in vivo, however additional research to evaluate related drugs is warranted. Published by Elsevier B.V. C1 [Wise, L. Nicki; Ueti, Massaro W.; Kappmeyer, Lowell S.; White, Stephen N.; Knowles, Donald P.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [Wise, L. Nicki; White, Stephen N.; Knowles, Donald P.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Hines, Melissa T.] Univ Tennessee, Coll Vet Med, Knoxville, TN 37996 USA. [Davis, Wendell] Bayer HealthCare LLC, Anim Hlth, Shawnee, KS 66216 USA. RP Wise, LN (reprint author), ARS, Anim Dis Res Unit, USDA, POB 646630, Pullman, WA 99164 USA. EM nwise@vetmed.wsu.edu OI White, Stephen N./0000-0001-9255-6054 FU Unites States Department of Agriculture, Agricultural Research Service [5348-32000-028-00D] FX This work was funded by the Unites States Department of Agriculture, Agricultural Research Service Project 5348-32000-028-00D. Ponazuril (Lot #T24438) was supplied by Bayer Healthcare LLC, Animal Health. NR 23 TC 5 Z9 5 U1 0 U2 7 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD APR 30 PY 2012 VL 185 IS 2-4 BP 282 EP 285 DI 10.1016/j.vetpar.2011.10.036 PG 4 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 926OC UT WOS:000302839700033 PM 22130334 ER PT J AU Xiang, WN Gobster, PH AF Xiang, Wei-Ning Gobster, Paul H. TI The new special issue policy and a call for proposals SO LANDSCAPE AND URBAN PLANNING LA English DT Editorial Material C1 [Xiang, Wei-Ning] E China Normal Univ, SIUES, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Shanghai 200062, Peoples R China. [Gobster, Paul H.] US Forest Serv, USDA, No Res Stn, Evanston, IL 60201 USA. RP Xiang, WN (reprint author), E China Normal Univ, SIUES, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Shanghai 200062, Peoples R China. EM wnxiang@mail.ecnu.edu.cn; pgobster@gmail.com RI Gobster, Paul/A-2826-2013 OI Gobster, Paul/0000-0002-8576-0310 NR 0 TC 1 Z9 1 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD APR 30 PY 2012 VL 105 IS 4 BP 345 EP 346 DI 10.1016/j.landurbplan.2012.02.014 PG 2 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 922AA UT WOS:000302518000001 ER PT J AU Washburn, BE AF Washburn, Brian E. TI Avian use of solid waste transfer stations (vol 104, pg 388, 2012) SO LANDSCAPE AND URBAN PLANNING LA English DT Correction C1 Wildlife Serv, USDA, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. RP Washburn, BE (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov NR 1 TC 0 Z9 0 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD APR 30 PY 2012 VL 105 IS 4 BP 446 EP 446 DI 10.1016/j.landurbplan.2012.02.002 PG 1 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 922AA UT WOS:000302518000012 ER PT J AU Knippenberg, MT Mikulski, PT Ryan, KE Stuart, SJ Gao, GT Harrison, JA AF Knippenberg, M. Todd Mikulski, Paul T. Ryan, Kathleen E. Stuart, Steven J. Gao, Guangtu Harrison, Judith A. TI Bond-order potentials with split-charge equilibration: Application to C-, H-, and O-containing systems SO JOURNAL OF CHEMICAL PHYSICS LA English DT Article ID MOLECULAR-DYNAMICS SIMULATIONS; ATOMIC-SCALE FRICTION; REACTIVE FORCE-FIELD; LIQUID WATER; DIMETHYL PEROXIDE; DIAMOND SURFACES; GRAIN-BOUNDARIES; TRIPLE JUNCTIONS; MODEL; HYDROCARBONS AB A method for extending charge transfer to bond-order potentials, known as the bond-order potential/split-charge equilibration (BOP/SQE) method [P. T. Mikulski, M. T. Knippenberg, and J. A. Harrison, J. Chem. Phys. 131, 241105 (2009)], is integrated into a new bond-order potential for interactions between oxygen, carbon, and hydrogen. This reactive potential utilizes the formalism of the adaptive intermolecular reactive empirical bond-order potential [S. J. Stuart, A. B. Tutein, and J. A. Harrison, J. Chem. Phys. 112, 6472 (2000)] with additional terms for oxygen and charge interactions. This implementation of the reactive potential is able to model chemical reactions where partial charges change in gas-and condensed-phase systems containing oxygen, carbon, and hydrogen. The BOP/SQE method prevents the unrestricted growth of charges, often observed in charge equilibration methods, without adding significant computational time, because it makes use of a quantity which is calculated as part of the underlying covalent portion of the potential, namely, the bond order. The implementation of this method with the qAIREBO potential is designed to provide a tool that can be used to model dynamics in a wide range of systems without significant computational cost. To demonstrate the usefulness and flexibility of this potential, heats of formation for isolated molecules, radial distribution functions of liquids, and energies of oxygenated diamond surfaces are calculated. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4704800] C1 [Knippenberg, M. Todd; Mikulski, Paul T.; Ryan, Kathleen E.; Harrison, Judith A.] USN Acad, Dept Phys, Annapolis, MD 21402 USA. [Knippenberg, M. Todd; Mikulski, Paul T.; Ryan, Kathleen E.; Harrison, Judith A.] USN Acad, Dept Chem, Annapolis, MD 21402 USA. [Knippenberg, M. Todd] High Point Univ, Dept Chem, High Point, NC 27262 USA. [Stuart, Steven J.] Clemson Univ, Dept Chem, Clemson, SC 29634 USA. [Gao, Guangtu] ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Knippenberg, MT (reprint author), USN Acad, Dept Phys, Annapolis, MD 21402 USA. RI Gao, Guangtu/F-4541-2012; Stuart, Steven/H-1111-2012 FU National Science Foundation [IAA 1129629]; Office of Naval Research (ONR) [N0001411WX21417]; AFOSR [F1ATA00130G001] FX J.A.H., P. T. M., and K. E. R. acknowledge support from the National Science Foundation IAA 1129629. M. T. K. also acknowledges partial support from the Office of Naval Research (ONR) Grant No. N0001411WX21417. G.A.O. and J.A.H. acknowledge partial support from AFOSR Grant No. F1ATA00130G001. The authors would also like to thank J. David Schall for helpful discussions. NR 71 TC 11 Z9 11 U1 3 U2 31 PU AMER INST PHYSICS PI MELVILLE PA CIRCULATION & FULFILLMENT DIV, 2 HUNTINGTON QUADRANGLE, STE 1 N O 1, MELVILLE, NY 11747-4501 USA SN 0021-9606 J9 J CHEM PHYS JI J. Chem. Phys. PD APR 28 PY 2012 VL 136 IS 16 AR 164701 DI 10.1063/1.4704800 PG 11 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA 936PJ UT WOS:000303602200037 PM 22559498 ER PT J AU Baxter, I Hermans, C Lahner, B Yakubova, E Tikhonova, M Verbruggen, N Chao, DY Salt, DE AF Baxter, Ivan Hermans, Christian Lahner, Brett Yakubova, Elena Tikhonova, Marina Verbruggen, Nathalie Chao, Dai-yin Salt, David E. TI Biodiversity of Mineral Nutrient and Trace Element Accumulation in Arabidopsis thaliana SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCUS; SERPENTINE SOILS; METAL HYPERACCUMULATION; FUNCTIONAL GENOMICS; NATURAL VARIATION; LEAF IONOME; TOLERANCE; PLANTS; IDENTIFICATION; POPULATIONS AB In order to grow on soils that vary widely in chemical composition, plants have evolved mechanisms for regulating the elemental composition of their tissues to balance the mineral nutrient and trace element bioavailability in the soil with the requirements of the plant for growth and development. The biodiversity that exists within a species can be utilized to investigate how regulatory mechanisms of individual elements interact and to identify genes important for these processes. We analyzed the elemental composition (ionome) of a set of 96 wild accessions of the genetic model plant Arabidopsis thaliana grown in hydroponic culture and soil using inductively coupled plasma mass spectrometry (ICP-MS). The concentrations of 17-19 elements were analyzed in roots and leaves from plants grown hydroponically, and leaves and seeds from plants grown in artificial soil. Significant genetic effects were detected for almost every element analyzed. We observed very few correlations between the elemental composition of the leaves and either the roots or seeds. There were many pairs of elements that were significantly correlated with each other within a tissue, but almost none of these pairs were consistently correlated across tissues and growth conditions, a phenomenon observed in several previous studies. These results suggest that the ionome of a plant tissue is variable, yet tightly controlled by genes and genexenvironment interactions. The dataset provides a valuable resource for mapping studies to identify genes regulating elemental accumulation. All of the ionomic data is available at www.ionomicshub.org. C1 [Baxter, Ivan] ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, USDA, St Louis, MO 63132 USA. [Baxter, Ivan; Lahner, Brett; Yakubova, Elena; Tikhonova, Marina; Chao, Dai-yin; Salt, David E.] Purdue Univ, Ctr Plant Stress Physiol, W Lafayette, IN 47907 USA. [Hermans, Christian; Verbruggen, Nathalie] Univ Libre Brussels, Lab Plant Physiol & Mol Genet, Brussels, Belgium. [Chao, Dai-yin; Salt, David E.] Univ Aberdeen, Inst Biol & Environm Sci, Aberdeen, Scotland. RP Baxter, I (reprint author), ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, USDA, St Louis, MO 63132 USA. EM ivan.baxter@ars.usda.gov RI Chao, Daiyin/A-5213-2013; Baxter, Ivan/A-1052-2009 OI Baxter, Ivan/0000-0001-6680-1722 FU National Institutes of Health, National Institute of General Medicine [R01 GM78536-01A1]; Indiana 21st Century Research and Technology Fund [912010479]; Belgian Science Policy Office (BelSPo) [IAPVI/33]; United States Department of Agriculture-Agricultural Research Service FX This project was funded by a grant from the National Institutes of Health, the National Institute of General Medicine (R01 GM78536-01A1) awarded to Dr. Salt, Dr. Guerinot and Dr. Baxter, the Indiana 21st Century Research and Technology Fund (912010479) to Dr. Salt, the Belgian Science Policy Office (BelSPo project IAPVI/33) to Dr. Verbruggen and United States Department of Agriculture-Agricultural Research Service Intramural funds to Dr. Baxter. Dr. Hermans is currently a research associate of the Fonds National de la Recherche Scientifique (FNRS-FRS). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 31 Z9 35 U1 7 U2 75 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 27 PY 2012 VL 7 IS 4 AR e35121 DI 10.1371/journal.pone.0035121 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TJ UT WOS:000305336000025 PM 22558123 ER PT J AU Schechter, LM Valenta, JC Schneider, DJ Collmer, A Sakk, E AF Schechter, Lisa M. Valenta, Joy C. Schneider, David J. Collmer, Alan Sakk, Eric TI Functional and Computational Analysis of Amino Acid Patterns Predictive Type III Secretion System Substrates in Pseudomonas syringae SO PLOS ONE LA English DT Article ID PV. TOMATO DC3000; ENTEROPATHOGENIC ESCHERICHIA-COLI; ENTERICA SEROTYPE TYPHIMURIUM; GRAM-NEGATIVE BACTERIA; YERSINIA-ENTEROCOLITICA; EFFECTOR PROTEINS; MESSENGER-RNA; PLANT-CELLS; PROTEOMIC ANALYSIS; TARGETING SIGNALS AB Bacterial type III secretion systems (T3SSs) deliver proteins called effectors into eukaryotic cells. Although N-terminal amino acid sequences are required for translocation, the mechanism of substrate recognition by the T3SS is unknown. Almost all actively deployed T3SS substrates in the plant pathogen Pseudomonas syringae pathovar tomato strain DC3000 possess characteristic patterns, including (i) greater than 10% serine within the first 50 amino acids, (ii) an aliphatic residue or proline at position 3 or 4, and (iii) a lack of acidic amino acids within the first 12 residues. Here, the functional significance of the P. syringae T3SS substrate compositional patterns was tested. A mutant AvrPto effector protein lacking all three patterns was secreted into culture and translocated into plant cells, suggesting that the compositional characteristics are not absolutely required for T3SS targeting and that other recognition mechanisms exist. To further analyze the unique properties of T3SS targeting signals, we developed a computational algorithm called TEREE (Type III Effector Relative Entropy Evaluation) that distinguishes DC3000 T3SS substrates from other proteins with a high sensitivity and specificity. Although TEREE did not efficiently identify T3SS substrates in Salmonella enterica, it was effective in another P. syringae strain and Ralstonia solanacearum. Thus, the TEREE algorithm may be a useful tool for identifying new effector genes in plant pathogens. The nature of T3SS targeting signals was additionally investigated by analyzing the N-terminus of FtsX, a putative membrane protein that was classified as a T3SS substrate by TEREE. Although the first 50 amino acids of FtsX were unable to target a reporter protein to the T3SS, an AvrPto protein substituted with the first 12 amino acids of FtsX was translocated into plant cells. These results show that the T3SS targeting signals are highly mutable and that secretion may be directed by multiple features of substrates. C1 [Schechter, Lisa M.; Valenta, Joy C.] Univ Missouri, Dept Biol, St Louis, MO 63121 USA. [Schneider, David J.] ARS, Plant Microbe Interact Res Unit, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY USA. [Schneider, David J.; Collmer, Alan] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Sakk, Eric] Morgan State Univ, Dept Comp Sci, Baltimore, MD 21239 USA. RP Schechter, LM (reprint author), Univ Missouri, Dept Biol, 8001 Nat Bridge Rd, St Louis, MO 63121 USA. EM schechterl@umsl.edu RI Schneider, David/H-2236-2012 OI Schneider, David/0000-0002-2124-8385 FU University of Misssouri-St. Louis; National Science Foundation [DBI-0077622]; United States Department of Agriculture-Agriculture Research Service [1907-21000-017-00] FX This work was supported by start-up research funds from the University of Misssouri-St. Louis, National Science Foundation award DBI-0077622, and United States Department of Agriculture-Agriculture Research Service project 1907-21000-017-00. The funders had no role in study design, data collection and analysis, decison to publish, or preparation of the manuscript. NR 84 TC 7 Z9 7 U1 0 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 27 PY 2012 VL 7 IS 4 AR e36038 DI 10.1371/journal.pone.0036038 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TJ UT WOS:000305336000109 PM 22558318 ER PT J AU Yan, CG Wang, XM Cao, J Wu, M Gao, HW AF Yan, Chunguang Wang, Ximo Cao, Jay Wu, Min Gao, Hongwei TI CCAAT/Enhancer-Binding Protein gamma Is a Critical Regulator of IL-1 beta-Induced IL-6 Production in Alveolar Epithelial Cells SO PLOS ONE LA English DT Article ID MONOCYTE CHEMOATTRACTANT PROTEIN-1; ACUTE LUNG INJURY; NF-KAPPA-B; C/EBP FAMILY; II CELLS; PROINFLAMMATORY CYTOKINES; AERUGINOSA INFECTION; INTERLEUKIN-6; EXPRESSION; MICE AB CCAAT/enhancer binding protein gamma (C/EBP gamma) is a member of the C/EBP family of transcription factors, which lacks known activation domains. C/EBP gamma was originally described as an inhibitor of C/EBP transactivation potential. However, previous study demonstrates that C/EBP gamma augments the C/EBP beta stimulatory activity in lipopolysaccharide induction of IL-6 promoter in a B lymphoblast cell line. These data indicate a complexing functional role for C/EBP gamma in regulating gene expression. Furthermore, the expression and function of C/EBP gamma during inflammation are still largely unknown. In this study, we demonstrate that C/EBP gamma activation was induced by IL-1 beta treatment in lung epithelial cells. Importantly, we demonstrate for the first time that C/EBP gamma plays a critical role in regulating IL-1 beta-induced IL-6 expression in both mouse primary alveolar type II epithelial cells and a lung epithelial cell line, MLE12. We further provide the evidence that C/EBP gamma inhibits IL-6 expression by inhibiting C/EBP gamma but not NF-kappa B stimulatory activity in MLE12 cells. These findings suggest that C/EBP gamma is a key transcription factor that regulates the IL-6 expression in alveolar epithelial cells, and may play an important regulatory role in lung inflammatory responses. C1 [Yan, Chunguang; Gao, Hongwei] Harvard Univ, Brigham & Womens Hosp, Ctr Expt Therapeut & Reperfus Injury, Dept Anesthesiol Perioperat & Pain Med,Sch Med, Boston, MA 02115 USA. [Yan, Chunguang; Wu, Min] Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58201 USA. [Wang, Ximo] Nankai Univ, Affiliated Hosp, Tianjin Union Med Ctr, Dept Surg, Tianjin 300071, Peoples R China. [Cao, Jay] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND USA. RP Yan, CG (reprint author), Harvard Univ, Brigham & Womens Hosp, Ctr Expt Therapeut & Reperfus Injury, Dept Anesthesiol Perioperat & Pain Med,Sch Med, Boston, MA 02115 USA. EM min.wu@med.und.edu; hgao@zeus.bwh.harvard.edu FU NIH [5R01HL092905-04, 3R01HL092905-02S1]; USDA Agricultural Research Service (ARS) [5450-51000-046-00D] FX This research was supported by NIH grants 5R01HL092905-04 and 3R01HL092905-02S1 (HG) and the USDA Agricultural Research Service (ARS) program "Mineral Intakes for Optimal Bone Development and Health," Current Research Information System (CRIS) no. 5450-51000-046-00D (JC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 6 Z9 6 U1 0 U2 0 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 27 PY 2012 VL 7 IS 4 AR e35492 DI 10.1371/journal.pone.0035492 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TJ UT WOS:000305336000033 PM 22558159 ER PT J AU Sylte, MJ Suarez, DL AF Sylte, Matthew J. Suarez, David L. TI Vaccination and acute phase mediator production in chickens challenged with low pathogenic avian influenza virus; novel markers for vaccine efficacy? SO VACCINE LA English DT Article DE Avian influenza; Low pathogenic; Vaccine efficacy; Alpha-1 acid glycoprotein; Prostaglandin E-2 and ovotransferrin ID ESCHERICHIA-COLI LIPOPOLYSACCHARIDE; PLASMA ALPHA-1-ACID GLYCOPROTEIN; C-REACTIVE PROTEIN; INFLAMMATORY AGENTS; ACID GLYCOPROTEIN; PROSTAGLANDIN E-2; SERUM-LEVELS; FEVER; INFECTION; INTERLEUKIN-6 AB Methods to determine vaccine efficacy of low pathogenic avian influenza (LPAI) isolates are limited in poultry because experimental infections with LPAI virus in specific pathogen free chickens rarely causes clinical disease. The most commonly used method to compare LPAI vaccine efficacy is to quantify viral shedding after challenge, but it is time and labor intensive. Therefore, we sought alternative methods to demonstrate vaccine efficacy, and examined whether vaccination of chickens affected the production of acute phase mediators in serum (e.g., alpha-1 acid glycoprotein, ovotransferrin and prostaglandin E-2) following challenge with homologous (A/Chicken/Hidalgo/232/94 H5N2) or heterologous (A/Chicken/167280-4/02 H5N3) LPAI isolates. Vaccination significantly reduced oropharyngeal viral shedding, and serum concentration of acute phase mediators, regardless of whether homologous or heterologous challenge viruses were used. Examining the expression of acute phase mediators post-challenge may serve as additional markers to determine LPAI vaccine efficacy in chickens. Published by Elsevier Ltd. C1 [Suarez, David L.] ARS, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. [Sylte, Matthew J.] Univ Georgia, Dept Infect Dis, Coll Vet Med, Athens, GA 30602 USA. RP Suarez, DL (reprint author), ARS, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM david.suarez@ars.usda.gov NR 48 TC 8 Z9 8 U1 3 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD APR 26 PY 2012 VL 30 IS 20 BP 3097 EP 3105 DI 10.1016/j.vaccine.2012.02.055 PG 9 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 932PK UT WOS:000303302800008 PM 22394994 ER PT J AU Pandya, M Pacheco, JM Bishop, E Kenney, M Milward, F Doel, T Golde, WT AF Pandya, Mital Pacheco, Juan M. Bishop, Elizabeth Kenney, Mary Milward, Francis Doel, Timothy Golde, William T. TI An alternate delivery system improves vaccine performance against foot-and-mouth disease virus (FMDV) SO VACCINE LA English DT Article DE FMDV; Vaccination; Intradermal; Virus; Immune response ID CATTLE; OUTBREAK; COSTS; PIGS; SKIN AB Foot-and-mouth disease virus (FMDV) causes vesicular disease of cloven-hoofed animals with severe agricultural and economic implications. One of the most highly infectious and contagious livestock pathogens known, the disease spreads rapidly in naive populations making it critical to have rapidly acting vaccines. Needle inoculation of killed virus vaccine is an efficient method of swiftly vaccinating large numbers of animals, either in eradication efforts or in outbreak situations in disease free countries, although, to be efficient, this requires utilizing the same needle with multiple animals. Here we present studies using a needle free system for vaccination with killed virus vaccine, FMDV strain O1 Manisa, as a rapid and consistent delivery platform. Cattle were vaccinated using a commercially available vaccine formulation at the manufacturer's recommended dose as well as four and sixteen fold less antigen load per dose. Animals were challenged intradermalingually (IDL) with live, virulent virus, homologous strain O1 Manisa, at various times following vaccination. All non-vaccinated control cattle exhibited clinical disease, including fever, viremia and lesions, specifically vesicle formation. Cattle vaccinated with the 1/16x and 1/4x doses using the needle free device were protected when challenged at both 7 and 28 days after vaccination. These data suggest that effective protection against disease can be achieved with 1/16 of the recommended vaccine dose when delivered using the needle free, intradermal delivery system, indicating the current vaccine stockpile that can be extended by many fold using this system. Published by Elsevier Ltd. C1 [Pandya, Mital; Pacheco, Juan M.; Bishop, Elizabeth; Kenney, Mary; Golde, William T.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. [Milward, Francis] Merial Anim Hlth, Athens, GA USA. [Doel, Timothy] Merial Anim Hlth, Pirbright, Surrey, England. RP Golde, WT (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY 11944 USA. EM william.golde@ARS.USDA.GOV OI Pacheco, Juan/0000-0001-5477-0201 NR 26 TC 7 Z9 7 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD APR 26 PY 2012 VL 30 IS 20 BP 3106 EP 3111 DI 10.1016/j.vaccine.2012.02.049 PG 6 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 932PK UT WOS:000303302800009 PM 22387223 ER PT J AU Duerr, AE Miller, TA Lanzone, M Brandes, D Cooper, J O'Malley, K Maisonneuve, C Tremblay, J Katzner, T AF Duerr, Adam E. Miller, Tricia A. Lanzone, Michael Brandes, Dave Cooper, Jeff O'Malley, Kieran Maisonneuve, Charles Tremblay, Junior Katzner, Todd TI Testing an Emerging Paradigm in Migration Ecology Shows Surprising Differences in Efficiency between Flight Modes SO PLOS ONE LA English DT Article ID FIELD OBSERVATIONS; SOUTHERN ISRAEL; SOARING FLIGHT; BIRD MORTALITY; WIND; SPEED; STRATEGIES; VULTURES; THERMALS; BEHAVIOR AB To maximize fitness, flying animals should maximize flight speed while minimizing energetic expenditure. Soaring speeds of large-bodied birds are determined by flight routes and tradeoffs between minimizing time and energetic costs. Large raptors migrating in eastern North America predominantly glide between thermals that provide lift or soar along slopes or ridgelines using orographic lift (slope soaring). It is usually assumed that slope soaring is faster than thermal gliding because forward progress is constant compared to interrupted progress when birds pause to regain altitude in thermals. We tested this slope-soaring hypothesis using high-frequency GPS-GSM telemetry devices to track golden eagles during northbound migration. In contrast to expectations, flight speed was slower when slope soaring and eagles also were diverted from their migratory path, incurring possible energetic costs and reducing speed of progress towards a migratory endpoint. When gliding between thermals, eagles stayed on track and fast gliding speeds compensated for lack of progress during thermal soaring. When thermals were not available, eagles minimized migration time, not energy, by choosing energetically expensive slope soaring instead of waiting for thermals to develop. Sites suited to slope soaring include ridges preferred for wind-energy generation, thus avian risk of collision with wind turbines is associated with evolutionary trade-offs required to maximize fitness of time-minimizing migratory raptors. C1 [Duerr, Adam E.; Miller, Tricia A.; Katzner, Todd] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Miller, Tricia A.] Penn State Univ, Riparia, University Pk, PA 16802 USA. [Lanzone, Michael] Cellular Tracking Technol LLC, Somerset, PA USA. [Brandes, Dave] Lafayette Coll, Dept Civil & Environm Engn, Acopian Engn Ctr, Easton, PA 18042 USA. [Cooper, Jeff] Virginia Dept Game & Inland Fisheries, Fredericksburg, VA USA. [O'Malley, Kieran] W Virginia Div Nat Resources, Romney, WV USA. [Maisonneuve, Charles] Minist Ressources Nat & Faune, Rimouski, PQ, Canada. [Tremblay, Junior] Minist Ressources Nat & Faune, Quebec City, PQ, Canada. [Katzner, Todd] US Forest Serv, USDA, Timber & Watershed Lab, Parsons, WV USA. RP Duerr, AE (reprint author), W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. EM adam.duerr@mail.wvu.edu FU Pennsylvania State Wildlife [T-12, T47-R-1]; United States Department of Energy [DE-EE0003538]; Virginia Department of Game and Inland Fisheries; Invenergy LLC; West Virginia Division of Natural Resources FX Funding was received from Pennsylvania State Wildlife Grants T-12 and T47-R-1, United States Department of Energy grant DE-EE0003538, the Virginia Department of Game and Inland Fisheries, Invenergy LLC, and the West Virginia Division of Natural Resources. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript that are not represented through coauthorship of this manuscript. NR 34 TC 26 Z9 26 U1 4 U2 62 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 25 PY 2012 VL 7 IS 4 AR e35548 DI 10.1371/journal.pone.0035548 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959VP UT WOS:000305345200047 PM 22558166 ER PT J AU Longcore, T Rich, C Mineau, P MacDonald, B Bert, DG Sullivan, LM Mutrie, E Gauthreaux, SA Avery, ML Crawford, RL Manville, AM Travis, ER Drake, D AF Longcore, Travis Rich, Catherine Mineau, Pierre MacDonald, Beau Bert, Daniel G. Sullivan, Lauren M. Mutrie, Erin Gauthreaux, Sidney A., Jr. Avery, Michael L. Crawford, Robert L. Manville, Albert M., II Travis, Emilie R. Drake, David TI An Estimate of Avian Mortality at Communication Towers in the United States and Canada SO PLOS ONE LA English DT Article ID FLORIDA TELEVISION TOWER; BIRD MORTALITY; NOCTURNAL MIGRANTS; GUY WIRES; TV TOWER; MIGRATION; COLLISIONS; SCAVENGERS; REMOVAL; WEATHER AB Avian mortality at communication towers in the continental United States and Canada is an issue of pressing conservation concern. Previous estimates of this mortality have been based on limited data and have not included Canada. We compiled a database of communication towers in the continental United States and Canada and estimated avian mortality by tower with a regression relating avian mortality to tower height. This equation was derived from 38 tower studies for which mortality data were available and corrected for sampling effort, search efficiency, and scavenging where appropriate. Although most studies document mortality at guyed towers with steady-burning lights, we accounted for lower mortality at towers without guy wires or steady-burning lights by adjusting estimates based on published studies. The resulting estimate of mortality at towers is 6.8 million birds per year in the United States and Canada. Bootstrapped subsampling indicated that the regression was robust to the choice of studies included and a comparison of multiple regression models showed that incorporating sampling, scavenging, and search efficiency adjustments improved model fit. Estimating total avian mortality is only a first step in developing an assessment of the biological significance of mortality at communication towers for individual species or groups of species. Nevertheless, our estimate can be used to evaluate this source of mortality, develop subsequent per-species mortality estimates, and motivate policy action. C1 [Longcore, Travis; Rich, Catherine] Urban Wildlands Grp, Los Angeles, CA USA. [Longcore, Travis] Univ So Calif, Spatial Sci Inst, Los Angeles, CA USA. [Mineau, Pierre; Bert, Daniel G.; Mutrie, Erin] Environm Canada, Sci & Technol Branch, Natl Wildlife Res Ctr, Ottawa, ON K1A 0H3, Canada. [Sullivan, Lauren M.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90024 USA. [Gauthreaux, Sidney A., Jr.] Clemson Univ, Dept Biol Sci, Clemson, SC 29634 USA. [Avery, Michael L.] Wildlife Serv, Natl Wildlife Res Ctr, Florida Field Stn, USDA, Gainesville, FL USA. [Manville, Albert M., II] US Fish & Wildlife Serv, Div Migratory Bird Management, Arlington, VA USA. [Travis, Emilie R.; Drake, David] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI USA. RP Longcore, T (reprint author), Urban Wildlands Grp, Los Angeles, CA USA. EM longcore@urbanwildlands.org RI Bert, Daniel /A-5666-2013; Longcore, Travis/A-4978-2008 OI Bert, Daniel /0000-0001-7922-6907; Longcore, Travis/0000-0002-1039-2613 FU American Bird Conservancy; Defenders of Wildlife FX Collaborators from The Urban Wildlands Group were supported in the early stages of this research by the American Bird Conservancy (www.abcbirds.org) and Defenders of Wildlife (www.defenders.org). The Canadian Wildlife Service (www.ec.gc.ca) purchased the NAV CANADA dataset. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 103 TC 38 Z9 40 U1 2 U2 45 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 25 PY 2012 VL 7 IS 4 AR e34025 DI 10.1371/journal.pone.0034025 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959VP UT WOS:000305345200007 PM 22558082 ER PT J AU Oppert, B Dowd, SE Bouffard, P Li, L Conesa, A Lorenzen, MD Toutges, M Marshall, J Huestis, DL Fabrick, J Oppert, C Jurat-Fuentes, JL AF Oppert, Brenda Dowd, Scot E. Bouffard, Pascal Li, Lewyn Conesa, Ana Lorenzen, Marce D. Toutges, Michelle Marshall, Jeremy Huestis, Diana L. Fabrick, Jeff Oppert, Cris Jurat-Fuentes, Juan Luis TI Transcriptome Profiling of the Intoxication Response of Tenebrio molitor Larvae to Bacillus thuringiensis Cry3Aa Protoxin SO PLOS ONE LA English DT Article ID TRIBOLIUM-CASTANEUM; CHORISTONEURA-FUMIFERANA; CRYSTAL PROTEINS; DELTA-ENDOTOXIN; CRY1AB TOXIN; RECEPTOR; IDENTIFICATION; RESISTANCE; SEQUENCE; MIDGUT AB Bacillus thuringiensis (Bt) crystal (Cry) proteins are effective against a select number of insect pests, but improvements are needed to increase efficacy and decrease time to mortality for coleopteran pests. To gain insight into the Bt intoxication process in Coleoptera, we performed RNA-Seq on cDNA generated from the guts of Tenebrio molitor larvae that consumed either a control diet or a diet containing Cry3Aa protoxin. Approximately 134,090 and 124,287 sequence reads from the control and Cry3Aa-treated groups were assembled into 1,318 and 1,140 contigs, respectively. Enrichment analyses indicated that functions associated with mitochondrial respiration, signalling, maintenance of cell structure, membrane integrity, protein recycling/synthesis, and glycosyl hydrolases were significantly increased in Cry3Aa-treated larvae, whereas functions associated with many metabolic processes were reduced, especially glycolysis, tricarboxylic acid cycle, and fatty acid synthesis. Microarray analysis was used to evaluate temporal changes in gene expression after 6, 12 or 24 h of Cry3Aa exposure. Overall, microarray analysis indicated that transcripts related to allergens, chitin-binding proteins, glycosyl hydrolases, and tubulins were induced, and those related to immunity and metabolism were repressed in Cry3Aa-intoxicated larvae. The 24 h microarray data validated most of the RNA-Seq data. Of the three intoxication intervals, larvae demonstrated more differential expression of transcripts after 12 h exposure to Cry3Aa. Gene expression examined by three different methods in control vs. Cry3Aa-treated larvae at the 24 h time point indicated that transcripts encoding proteins with chitin-binding domain 3 were the most differentially expressed in Cry3Aa-intoxicated larvae. Overall, the data suggest that T. molitor larvae mount a complex response to Cry3Aa during the initial 24 h of intoxication. Data from this study represent the largest genetic sequence dataset for T. molitor to date. Furthermore, the methods in this study are useful for comparative analyses in organisms lacking a sequenced genome. C1 [Oppert, Brenda; Toutges, Michelle] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. [Dowd, Scot E.] Mol Res LP, Shallowater, TX USA. [Bouffard, Pascal; Li, Lewyn] 454 Life Sci, Branford, CT USA. [Conesa, Ana] Ctr Invest Principe Felipe, Bioinformat & Genom Dept, Valencia, Spain. [Lorenzen, Marce D.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27695 USA. [Marshall, Jeremy; Huestis, Diana L.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Fabrick, Jeff] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Oppert, Cris; Jurat-Fuentes, Juan Luis] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37901 USA. RP Oppert, B (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. EM bso@ksu.edu RI Conesa Cegarra, Ana/A-9559-2014; OI Conesa Cegarra, Ana/0000-0001-9597-311X; Huestis, Diana/0000-0001-6649-4785 FU USDA National Institute of Food and Agriculture [2008-39211-19577] FX CO was supported by Biotechnology Risk Assessment Program Competitive Grant no. 2008-39211-19577 from the USDA National Institute of Food and Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 18 Z9 19 U1 3 U2 54 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 25 PY 2012 VL 7 IS 4 AR e34624 DI 10.1371/journal.pone.0034624 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959VP UT WOS:000305345200011 PM 22558093 ER PT J AU Park, JB Wang, TTY AF Park, Jae B. Wang, Thomas T. Y. TI Safflomide Increases the Expression of Adiponectin in Vitro and in Vivo: Potential Implication for Hypoadiponectemia, Visceral Obesity, and Insulin Resistance SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Safflomide; antioxidant; adiponectin; serotonin receptor; obesity; insulin resistance ID METABOLIC SYNDROME; ADIPOKINES; RECEPTOR; CYTOKINES; CELLS AB Safflomide (N-caffeoyltryptamine) is a phenolic amide with serotonin receptor antagonist and antioxidant activities. We investigated the potential effects of safflomide on the expression of adipokines in vitro and in vivo. Safflomide did not affect the expressions of TNF-alpha, IL-6, and MCP-1/CCL2 in hypertrophic 3T3-L1 cells but upregulated adiponectin mRNA 1-5-fold at concentrations between 1 and 20 mu M (p < 0.05). Because safflomide is a non-selective 5-HT receptor antagonist and because the expression of 5-HT2A receptor is often inversely correlated to adiponectin expression, the potential effects of 5-HT receptor antagonist activity of safflomide on the expression of adiponectin was further investigated in 3T3-L1 cells. At the concentration of 10 mu M, safflomide was able to increase adiponectin protein production in 3T3-L1 cells more than 4-fold (p < 0.05), which was greater than the 5-HT2A antagonist ketanserin. The upregulation was partially suppressed by treatment with 5-HT2A agonists (serotonin and alpha-Me-5-HT), suggesting that safflomide may upregulate adiponectin expression more than by blocking 5-HT2A receptors in 3T3-L1 cells. Likely, the upregulation was also attributed to the antioxidant activity of safflomide because two safflomide analogues (N-cinnamoyltryptamine and N-coumaroyltryptamine) with less antioxidant activity were not as potent as safflomide. Rats supplemented with safflomide (3 mg/day) in a high-fat diet showed a significant plasma adiponectin increase (more than 30%) with a significant reduction in body weight, visceral fat, and improved insulin resistance compared to non-supplemented rats, demonstrating the in vivo activity of safflomide. These data suggest that safflomide may have beneficial effects on obesity-related conditions, such as low adiponectin, visceral obesity, and insulin resistance. C1 [Park, Jae B.; Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, BHNRC, USDA, Beltsville, MD 20705 USA. RP Park, JB (reprint author), ARS, Diet Genom & Immunol Lab, BHNRC, USDA, Room 131,Bldg 307C, Beltsville, MD 20705 USA. EM jae.park@ars.usda.gov NR 30 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 25 PY 2012 VL 60 IS 16 BP 4048 EP 4052 DI 10.1021/jf2044108 PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 930KZ UT WOS:000303139600009 PM 22428927 ER PT J AU Seidl, R Rammer, W Scheller, RM Spies, TA AF Seidl, Rupert Rammer, Werner Scheller, Robert M. Spies, Thomas A. TI An individual-based process model to simulate landscape-scale forest ecosystem dynamics SO ECOLOGICAL MODELLING LA English DT Article DE Forest ecosystem dynamics; Complex adaptive systems; Individual-based modeling; Ecological field theory; Hierarchical multi-scale modeling; Forest structure and functioning ID ECOLOGICAL FIELD-THEORY; TREE MORTALITY MODELS; GROSS PRIMARY PRODUCTION; NET PRIMARY PRODUCTION; CLIMATE-CHANGE; CO2 FERTILIZATION; CARBON FLUXES; GROWTH-MODEL; DOUGLAS-FIR; GAP MODELS AB Forest ecosystem dynamics emerges from nonlinear interactions between adaptive biotic agents (i.e., individual trees) and their relationship with a spatially and temporally heterogeneous abiotic environment. Understanding and predicting the dynamics resulting from these complex interactions is crucial for the sustainable stewardship of ecosystems, particularly in the context of rapidly changing environmental conditions. Here we present iLand (the individual-based forest landscape and disturbance model), a novel approach to simulating forest dynamics as an emergent property of environmental drivers, ecosystem processes and dynamic interactions across scales. Our specific objectives were (i) to describe the model, in particular its novel approach to simulate spatially explicit individual-tree competition for resources over large scales within a process-based framework of physiological resource use, and (ii) to present a suite of evaluation experiments assessing iLands ability to simulate tree growth and mortality for a wide range of forest ecosystems. Adopting an approach rooted in ecological field theory, iLand calculates a continuous field of light availability over the landscape, with every tree represented by a mechanistically derived, size- and species-dependent pattern of light interference. Within a hierarchical multi-scale framework productivity is derived at stand-level by means of a light-use efficiency approach, and downscaled to individuals via local light availability. Allocation (based on allometric ratios) and mortality (resulting from carbon starvation) are modeled at the individual-tree level, accounting for adaptive behavior of trees in response to their environment. To evaluate the model we conducted simulations over the extended environmental gradient of a longitudinal transect in Oregon, USA, and successfully compared results against independently observed productivity estimates (63.4% of variation explained) and mortality patterns in even-aged stands. This transect experiment was furthermore replicated for a different set of species and ecosystems in the Austrian Alps, documenting the robustness and generality of our approach. Model performance was also successfully evaluated for structurally and compositionally complex old-growth forests in the western Cascades of Oregon. Finally, the ability of our approach to address forest ecosystem dynamics at landscape scales was demonstrated by a computational scaling experiment. In simulating the emergence of ecosystem patterns and dynamics as a result of complex process interactions across scales our approach has the potential to contribute crucial capacities to understanding and fostering forest ecosystem resilience under changing climatic conditions. 2012 Elsevier B.V. All rights reserved. C1 [Seidl, Rupert; Rammer, Werner] Univ Nat Resources & Life Sci BOKU Vienna, Inst Silviculture, Vienna, Austria. [Seidl, Rupert] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Scheller, Robert M.] Portland State Univ, Portland, OR 97207 USA. [Spies, Thomas A.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR USA. RP Seidl, R (reprint author), Univ Nat Resources & Life Sci BOKU Vienna, Inst Silviculture, Vienna, Austria. EM rupert.seidl@boku.ac.at RI Scheller, Robert/B-3135-2009; Seidl, Rupert/A-4858-2013; McLean, Laurence /C-7367-2014; OI Seidl, Rupert/0000-0002-3338-3402 FU European Community [237085] FX This work was funded by a Marie Curie Fellowship awarded to R. Seidl under the European Community's Seventh Framework Program (grant agreement 237085). We are grateful to M.J. Lexer for administrative support, to the H.J. Andrews community for making available their data for this study, and in particular thank R.J. Pabst for assistance with the reference stand data. We furthermore thank R.H. Waring for insightful discussions and C. Schauflinger for the professional rendering of the model schematics in Fig. 1. M.E. Harmon and two anonymous reviewers are gratefully acknowledged for providing helpful comments on an earlier version of the manuscript. NR 129 TC 40 Z9 43 U1 3 U2 84 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 J9 ECOL MODEL JI Ecol. Model. PD APR 24 PY 2012 VL 231 BP 87 EP 100 DI 10.1016/j.ecolmodel.2012.02.015 PG 14 WC Ecology SC Environmental Sciences & Ecology GA 929QT UT WOS:000303081300009 ER PT J AU Cushman, SA Landguth, EL AF Cushman, Samuel A. Landguth, Erin L. TI Multi-taxa population connectivity in the Northern Rocky Mountains SO ECOLOGICAL MODELLING LA English DT Article DE Connectivity; Corridor; Animal movement; Resistant kernel; Umbrella species ID GENE FLOW; CLIMATE-CHANGE; MOVEMENT CORRIDORS; LANDSCAPE GENETICS; CONSERVATION; UMBRELLA; MODEL; FRAGMENTATION; AMPHIBIANS; CARNIVORES AB Effective broad-spectrum biodiversity conservation requires that conservation strategies simultaneously meet the needs of multiple species. However, little is known about how maintaining habitat connectivity for one species or species group may also act as an umbrella for other species. We evaluated the degree to which predicted connected habitat for each of 144 different hypothetical organisms expressing range of dispersal abilities and ecological responses to elevation, roads and land cover function as an indicators of connected habitat for the others in the U.S. Northern Rocky Mountains. We used resistant kernel modeling to map the extent of the study area predicted to be connected by dispersal for each species. At relatively large dispersal abilities there was extensive overlap between connected habitat for most organisms and much of the study area is predicted to provide connected habitat for all hypothetical organisms simultaneously. In contrast, at low to medium dispersal abilities there was much less intersection of habitat connected by dispersal. We found that habitat specialists with limited dispersal ability are weak indicators of others, and likewise are weakly indicated by others. We evaluated the effectiveness of three carnivores as connectivity umbrellas for many species. All three carnivore species performed significantly worse as connectivity umbrellas than the average across the simulated species. These species are associated with high elevation forested habitats. It is the low elevation and non-forest habitats that are most at risk of habitat loss and fragmentation in the study area, suggesting that a carnivore umbrella may miss many species most at risk. Published by Elsevier B.V. C1 [Cushman, Samuel A.] US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Landguth, Erin L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Cushman, SA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM scushman@fs.fed.us NR 51 TC 20 Z9 20 U1 4 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 J9 ECOL MODEL JI Ecol. Model. PD APR 24 PY 2012 VL 231 BP 101 EP 112 DI 10.1016/j.ecolmodel.2012.02.011 PG 12 WC Ecology SC Environmental Sciences & Ecology GA 929QT UT WOS:000303081300010 ER PT J AU DeJonge, KC Ascough, JC Ahmadi, M Andales, AA Arabi, M AF DeJonge, Kendall C. Ascough, James C., II Ahmadi, Mehdi Andales, Allan A. Arabi, Mazdak TI Global sensitivity and uncertainty analysis of a dynamic agroecosystem model under different irrigation treatments SO ECOLOGICAL MODELLING LA English DT Article DE Global sensitivity analysis; Uncertainty analysis; CERES-Maize; Limited irrigation; Morris sensitivity method; Sobol' sensitivity method ID CERES-MAIZE MODEL; MEDITERRANEAN ENVIRONMENT; SOIL; PARAMETERS; YIELD; GROWTH; CORN; SIMULATION; MANAGEMENT; RADIATION AB Savings in consumptive use through limited or deficit irrigation in agriculture has become an increasingly viable source of additional water for places with high population growth such as the Colorado Front Range, USA. Crop models provide a mechanism to evaluate various management methods without performing costly and time-consuming experiments, e.g., field studies investigating irrigation scheduling and timing effects on crop growth. Few studies have focused on CERES-Maize crop model parameterization with respect to water-stressed conditions, and the model has previously been shown to overestimate evapotranspiration (ET) for limited irrigation treatments (stress during vegetative stage). It is therefore desirable to quantify the effects of CERES-Maize input parameters on model output responses typically used for calibration and/or important in limited irrigation management, including vegetative growth, crop yield, and ET. A sensitivity analysis (SA) utilizing the Morris one-at-a-time screening and Sobol' variance-based methods was performed on CERES-Maize v4.5 input parameters affecting water balance and crop growth including soil hydraulic properties, phenological growth properties, and radiation use efficiency. CERES-Maize output responses of interest for the SA included anthesis date, maturity date, leaf number per stem, maximum leaf area index, yield, and cumulative ET. The SA study utilized five years of multi-replicate field management data (both full and limited irrigation treatments) for each combination of random input parameters. Results comparing the Morris mean and the Sobol' total sensitivity index showed very high correlation between the two, indicating that in this study the computationally cheaper Morris method could have been used as an effective indicator of input parameter sensitivity. For the full irrigation treatment, CERES-Maize output responses were mostly sensitive to crop cultivar parameters. For the limited irrigation treatment, CERES-Maize leaf area index, yield, and ET output responses were highly influenced by soil lower limit and drained upper limit input parameters, which define water holding capacity. There was also a greater amount of interaction between input parameters for the limited irrigation treatment than for full irrigation. An uncertainty analysis was also conducted using model outputs from the Sobol' SA method. In some cases, cumulative ET had higher values for limited irrigation than for full irrigation, further indicating the need to evaluate model processes governing Er under water stress. A new methodology for systematic calibration of CERES-Maize, based on the Morris and Sobol' sensitivity indices for the two irrigation treatments, is proposed for future model evaluation as sensitivity differences between treatments indicates that existing CERES-Maize calibration procedures (typically based on non-stressed crops) may need to be reconsidered in cases of water stress. Published by Elsevier B.V. C1 [DeJonge, Kendall C.; Ahmadi, Mehdi; Arabi, Mazdak] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Ascough, James C., II] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. [Andales, Allan A.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. RP DeJonge, KC (reprint author), USDA ARS, Water Management Res Unit, 2150 Ctr Ave,Bldg D,Suite 320, Ft Collins, CO 80526 USA. EM kendall.dejonge@ars.usda.gov RI Andales, Allan/M-8328-2013 NR 64 TC 29 Z9 34 U1 2 U2 43 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 J9 ECOL MODEL JI Ecol. Model. PD APR 24 PY 2012 VL 231 BP 113 EP 125 DI 10.1016/j.ecolmodel.2012.01.024 PG 13 WC Ecology SC Environmental Sciences & Ecology GA 929QT UT WOS:000303081300011 ER PT J AU Krug, PW Larson, CR Eslami, AC Rodriguez, LL AF Krug, Peter W. Larson, Christopher R. Eslami, Angelique C. Rodriguez, Luis L. TI Disinfection of foot-and-mouth disease and African swine fever viruses with citric acid and sodium hypochlorite on birch wood carriers SO VETERINARY MICROBIOLOGY LA English DT Article DE Transboundary disease; Foot-and-mouth; Swine fever; Surface disinfection ID CHEMICAL DISINFECTANTS; NONPOROUS SURFACES; VIRUCIDAL ACTIVITY; SURVIVAL; INACTIVATION; FOMITES AB Transboundary animal disease viruses such as foot-and-mouth disease virus (FMDV) and African swine fever virus (ASFV) are highly contagious and cause severe morbidity and mortality in livestock. Proper disinfection during an outbreak can help prevent virus spread and will shorten the time for contaminated agriculture facilities to return to food production. Wood surfaces are prevalent at these locations, but there is no standardized method for porous surface disinfection; commercial disinfectants are only certified for use on hard, nonporous surfaces. To model porous surface disinfection in the laboratory, FMDV and ASFV stocks were dried on wood coupons and exposed to citric acid or sodium hypochlorite. We found that 2% citric acid was effective at inactivating both viruses dried on a wood surface by 30 min at 22 degrees C. While 2000 ppm sodium hypochlorite was capable of inactivating ASFV on wood under these conditions, this chemical did not meet the 4-log disinfection threshold for FMDV. Taken together, our data supports the use of chemical disinfectants containing at least 2% citric acid for porous surface disinfection of FMDV and ASFV. Published by Elsevier B.V. C1 [Krug, Peter W.; Larson, Christopher R.; Eslami, Angelique C.; Rodriguez, Luis L.] ARS, USDA, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, Greenport, NY 11944 USA. [Larson, Christopher R.; Eslami, Angelique C.] Oak Ridge Inst Sci & Educ, Plum Isl Anim Dis Ctr, Res Participat Program, Oak Ridge, TN USA. RP Krug, PW (reprint author), ARS, USDA, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, POB 848, Greenport, NY 11944 USA. EM peter.krug@ars.usda.gov FU EPA-USDA [60-1940-8-055] FX This work was funded by EPA-USDA Interagency agreement number 60-1940-8-055. CRL and ACE were participants of the Plum Island Animal Disease Center Research Participation Program administered by the Oak Ridge Institute for Science and Education. NR 27 TC 3 Z9 6 U1 6 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD APR 23 PY 2012 VL 156 IS 1-2 BP 96 EP 101 DI 10.1016/j.vetmic.2011.10.032 PG 6 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 917OR UT WOS:000302188100010 PM 22115968 ER PT J AU Verde, I Bassil, N Scalabrin, S Gilmore, B Lawley, CT Gasic, K Micheletti, D Rosyara, UR Cattonaro, F Vendramin, E Main, D Aramini, V Blas, AL Mockler, TC Bryant, DW Wilhelm, L Troggio, M Sosinski, B Aranzana, MJ Arus, P Iezzoni, A Morgante, M Peace, C AF Verde, Ignazio Bassil, Nahla Scalabrin, Simone Gilmore, Barbara Lawley, Cynthia T. Gasic, Ksenija Micheletti, Diego Rosyara, Umesh R. Cattonaro, Federica Vendramin, Elisa Main, Dorrie Aramini, Valeria Blas, Andrea L. Mockler, Todd C. Bryant, Douglas W. Wilhelm, Larry Troggio, Michela Sosinski, Bryon Jose Aranzana, Maria Arus, Pere Iezzoni, Amy Morgante, Michele Peace, Cameron TI Development and Evaluation of a 9K SNP Array for Peach by Internationally Coordinated SNP Detection and Validation in Breeding Germplasm SO PLOS ONE LA English DT Article ID PRUNUS-PERSICA CULTIVARS; LINKAGE DISEQUILIBRIUM; GENOTYPING ASSAY; L. BATSCH; TECHNOLOGY; GENOME; QTLS; DAVIDIANA; GRAPEVINE; QUALITY AB Although a large number of single nucleotide polymorphism (SNP) markers covering the entire genome are needed to enable molecular breeding efforts such as genome wide association studies, fine mapping, genomic selection and marker-assisted selection in peach [Prunus persica (L.) Batsch] and related Prunus species, only a limited number of genetic markers, including simple sequence repeats (SSRs), have been available to date. To address this need, an international consortium (The International Peach SNP Consortium; IPSC) has pursued a coordinated effort to perform genome-scale SNP discovery in peach using next generation sequencing platforms to develop and characterize a high-throughput Illumina Infinium (R) SNP genotyping array platform. We performed whole genome re-sequencing of 56 peach breeding accessions using the Illumina and Roche/454 sequencing technologies. Polymorphism detection algorithms identified a total of 1,022,354 SNPs. Validation with the Illumina GoldenGate (R) assay was performed on a subset of the predicted SNPs, verifying similar to 75% of genic (exonic and intronic) SNPs, whereas only about a third of intergenic SNPs were verified. Conservative filtering was applied to arrive at a set of 8,144 SNPs that were included on the IPSC peach SNP array v1, distributed over all eight peach chromosomes with an average spacing of 26.7 kb between SNPs. Use of this platform to screen a total of 709 accessions of peach in two separate evaluation panels identified a total of 6,869 (84.3%) polymorphic SNPs. The almost 7,000 SNPs verified as polymorphic through extensive empirical evaluation represent an excellent source of markers for future studies in genetic relatedness, genetic mapping, and dissecting the genetic architecture of complex agricultural traits. The IPSC peach SNP array v1 is commercially available and we expect that it will be used worldwide for genetic studies in peach and related stone fruit and nut species. C1 [Verde, Ignazio; Vendramin, Elisa; Aramini, Valeria] Fruit Tree Res Ctr, Rome, Italy. [Bassil, Nahla; Gilmore, Barbara] ARS, USDA, Corvallis, OR USA. [Scalabrin, Simone; Cattonaro, Federica; Morgante, Michele] Ist Genom Applicata, Udine, Italy. [Lawley, Cynthia T.] Illumina Inc, Hayward, CA USA. [Gasic, Ksenija] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC USA. [Micheletti, Diego; Blas, Andrea L.; Jose Aranzana, Maria; Arus, Pere] Ctr Recerca Agrigen CSIC IRTA UAB UB, IRTA, Barcelona, Spain. [Rosyara, Umesh R.; Iezzoni, Amy] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Main, Dorrie; Peace, Cameron] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. [Mockler, Todd C.; Bryant, Douglas W.] Donald Danforth Plant Sci Ctr, St Louis, MO USA. [Mockler, Todd C.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Mockler, Todd C.] Oregon State Univ, Ctr Genome Res & Biocomp, Corvallis, OR 97331 USA. [Bryant, Douglas W.] Intuit Genom Inc, St Louis, MO USA. [Wilhelm, Larry] Oregon Hlth & Sci Univ, Portland, OR USA. [Troggio, Michela] Fdn Edmund Mach, Ist Agr San Michele Adige Res & Innovat Ct, San Michele All Adige, Italy. [Sosinski, Bryon] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Morgante, Michele] Univ Udine, Dipartimento Sci Agr & Ambientali, I-33100 Udine, Italy. RP Verde, I (reprint author), Fruit Tree Res Ctr, Rome, Italy. EM ignazio.verde@entecra.it; cpeace@wsu.edu RI Arus, Pere/F-6443-2015; Aranzana, Maria Jose/H-7799-2015; Mockler, Todd/L-2609-2013; OI Arus, Pere/0000-0003-0939-8038; Aranzana, Maria Jose/0000-0002-0240-8021; Mockler, Todd/0000-0002-0462-5775; Verde, Ignazio/0000-0002-9139-955X; Vendramin, Elisa/0000-0002-7324-7237 FU United States Department of Agriculture's National Institute of Food and Agriculture [2009-51181-05808]; Ministero delle Politiche Agricole Alimentari e Forestali -Italy (MiPAAF) [DM14999/7303/08]; European Union [FP7-265582]; Spanish Ministry of Science and Innovation [AGL2009-07305, CSD2007-00036] FX This work was funded by: United States Department of Agriculture's National Institute of Food and Agriculture - Specialty Crop Research Initiative project (http://www.csrees.usda.gov/fo/specialtycropresearchinitiative.cfm), "RosBREED: Enabling marker-assisted breeding in Rosaceae" (Grant # 2009-51181-05808); Ministero delle Politiche Agricole Alimentari e Forestali -Italy (MiPAAF, http://www.politicheagricole.it) through the project "DRUPOMICS: Sequenziamento del genoma del pesco ed utilizzo della sequenza in programmi di miglioramento della qualita del frutto del pesco e della resistenza alle malattie" (Grant # DM14999/7303/08). European Union-funded project "FruitBreedomics: Integrated approach for increasing breeding efficiency in fruit tree crops" (Grant # FP7-265582; http://fruitbreedomics.com/; http://ec.europa.eu/research/fp7/index_en.cfm). AGL2009-07305 project and Consolider-Ingenio 2010 Program project (grant # CSD2007-00036) both funded by the Spanish Ministry of Science and Innovation (http://www.micinn.es/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 26 TC 89 Z9 92 U1 1 U2 51 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 20 PY 2012 VL 7 IS 4 AR e35668 DI 10.1371/journal.pone.0035668 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UD UT WOS:000305339200093 PM 22536421 ER PT J AU Wu, ST Li, RW Li, WZ Beshah, E Dawson, HD Urban, JF AF Wu, Sitao Li, Robert W. Li, Weizhong Beshah, Ethiopia Dawson, Harry D. Urban, Joseph F., Jr. TI Worm Burden-Dependent Disruption of the Porcine Colon Microbiota by Trichuris suis Infection SO PLOS ONE LA English DT Article ID INFLAMMATORY-BOWEL-DISEASE; ALTERNATIVELY ACTIVATED MACROPHAGES; CAMPYLOBACTER-JEJUNI; SWINE; EXPRESSION; INDUCTION; ULTRAFAST; SEQUENCES; NEMATODES; ALIGNMENT AB Helminth infection in pigs serves as an excellent model for the study of the interaction between human malnutrition and parasitic infection and could have important implications in human health. We had observed that pigs infected with Trichuris suis for 21 days showed significant changes in the proximal colon microbiota. In this study, interactions between worm burden and severity of disruptions to the microbial composition and metabolic potentials in the porcine proximal colon microbiota were investigated using metagenomic tools. Pigs were infected by a single dose of T. suis eggs for 53 days. Among infected pigs, two cohorts were differentiated that either had adult worms or were worm-free. Infection resulted in a significant change in the abundance of approximately 13% of genera detected in the proximal colon microbiota regardless of worm status, suggesting a relatively persistent change over time in the microbiota due to the initial infection. A significant reduction in the abundance of Fibrobacter and Ruminococcus indicated a change in the fibrolytic capacity of the colon microbiota in T. suis infected pigs. In addition, similar to 10% of identified KEGG pathways were affected by infection, including ABC transporters, peptidoglycan biosynthesis, and lipopolysaccharide biosynthesis as well as alpha-linolenic acid metabolism. Trichuris suis infection modulated host immunity to Campylobacter because there was a 3-fold increase in the relative abundance in the colon microbiota of infected pigs with worms compared to naive controls, but a 3-fold reduction in worm-free infected pigs compared to controls. The level of pathology observed in infected pigs with worms compared to worm-free infected pigs may relate to the local host response because expression of several Th2-related genes were enhanced in infected pigs with worms versus those worm-free. Our findings provided insight into the dynamics of the proximal colon microbiota in pigs in response to T. suis infection. C1 [Li, Robert W.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD USA. [Wu, Sitao; Li, Weizhong] Univ Calif San Diego, Ctr Res Biol Syst, San Diego, CA 92103 USA. [Beshah, Ethiopia; Dawson, Harry D.; Urban, Joseph F., Jr.] ARS, USDA, Diet Genom & Immunol Lab, Beltsville, MD USA. RP Li, RW (reprint author), ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD USA. EM robert.li@ars.usda.gov RI Dawson, Harry/H-8242-2013; Li, Weizhong/A-9735-2008; OI Li, Weizhong/0000-0003-1804-9403; Urban, Joseph/0000-0002-1590-8869 FU National Human Genome Research Institute (NHGRI) [R01HG005978]; National Center for Research Resources (NCRR) [R01RR025030]; CAMERA project; Gordon and Betty Moore Foundation FX SW and WL were supported by Award R01HG005978 from the National Human Genome Research Institute (NHGRI), Award R01RR025030 from National Center for Research Resources (NCRR) and CAMERA project funded by Gordon and Betty Moore Foundation. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NHGRI, NCRR or the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 34 Z9 35 U1 1 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 20 PY 2012 VL 7 IS 4 AR e35470 DI 10.1371/journal.pone.0035470 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UD UT WOS:000305339200068 PM 22532855 ER PT J AU Olien, CR AF Olien, C. Robert TI The equilibrated state of freezing as a basis for distinguishing lethal stresses of freezing in plants SO THERMOCHIMICA ACTA LA English DT Article DE Winterhardiness; Freeze stress; Interfacial water; Intercellular ice ID ENERGIES; WATER AB A model for coordination of stresses that limit winterhardiness in plants based on the thermodynamic equilibrated state of freezing and melting provides a rational basis for distinction of freeze-induced energies which can stress and injure living organisms in various ways. The departure from equilibrium, other than the energy required for maintenance of the steady state, is the free energy of freeze stress when defined by forces that interfere with essential function or disrupt essential structure in plant tissue. Published by Elsevier B.V. C1 Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. RP Olien, CR (reprint author), Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. EM stiefelm@msu.edu NR 17 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0040-6031 J9 THERMOCHIM ACTA JI Thermochim. Acta PD APR 20 PY 2012 VL 534 BP 17 EP 20 DI 10.1016/j.tca.2012.01.022 PG 4 WC Thermodynamics; Chemistry, Analytical; Chemistry, Physical SC Thermodynamics; Chemistry GA 928IN UT WOS:000302976500003 ER PT J AU Regier, JC Brown, JW Mitter, C Baixeras, J Cho, S Cummings, MP Zwick, A AF Regier, Jerome C. Brown, John W. Mitter, Charles Baixeras, Joaquin Cho, Soowon Cummings, Michael P. Zwick, Andreas TI A Molecular Phylogeny for the Leaf-Roller Moths (Lepidoptera: Tortricidae) and Its Implications for Classification and Life History Evolution SO PLOS ONE LA English DT Article ID CODING NUCLEAR GENES; SPECIES LEPIDOPTERA; BIOLOGICAL-CONTROL; FEMALE FRENULUM; NEW-CALEDONIA; MISSING DATA; GENUS; BUTTERFLIES; CHLIDANOTINAE; PHRICANTHINI AB Background: Tortricidae, one of the largest families of microlepidopterans, comprise about 10,000 described species worldwide, including important pests, biological control agents and experimental models. Understanding of tortricid phylogeny, the basis for a predictive classification, is currently provisional. We present the first detailed molecular estimate of relationships across the tribes and subfamilies of Tortricidae, assess its concordance with previous morphological evidence, and re-examine postulated evolutionary trends in host plant use and biogeography. Methodology/Principal Findings: We sequenced up to five nuclear genes (6,633 bp) in each of 52 tortricids spanning all three subfamilies and 19 of the 22 tribes, plus up to 14 additional genes, for a total of 14,826 bp, in 29 of those taxa plus all 14 outgroup taxa. Maximum likelihood analyses yield trees that, within Tortricidae, differ little among data sets and character treatments and are nearly always strongly supported at all levels of divergence. Support for several nodes was greatly increased by the additional 14 genes sequenced in just 29 of 52 tortricids, with no evidence of phylogenetic artifacts from deliberately incomplete gene sampling. There is strong support for the monophyly of Tortricinae and of Olethreutinae, and for grouping of these to the exclusion of Chlidanotinae. Relationships among tribes are robustly resolved in Tortricinae and mostly so in Olethreutinae. Feeding habit (internal versus external) is strongly conserved on the phylogeny. Within Tortricinae, a clade characterized by eggs being deposited in large clusters, in contrast to singly or in small batches, has markedly elevated incidence of polyphagous species. The five earliest-branching tortricid lineages are all species-poor tribes with mainly southern/tropical distributions, consistent with a hypothesized Gondwanan origin for the family. Conclusions/Significance: We present the first robustly supported phylogeny for Tortricidae, and a revised classification in which all of the sampled tribes are now monophyletic. C1 [Regier, Jerome C.; Mitter, Charles] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Regier, Jerome C.] Inst Biosci & Biotechnol Res, College Pk, MD USA. [Brown, John W.] ARS, Systemat Entomol Lab, USDA, Beltsville, MD USA. [Baixeras, Joaquin] Univ Valencia, Cavanilles Inst Biodivers & Evolutionary Biol, Valencia, Spain. [Cho, Soowon] Chungbuk Natl Univ, Dept Plant Med, Cheongju, South Korea. [Cummings, Michael P.] Univ Maryland, Lab Mol Evolut, Ctr Bioinformat & Computat Biol, College Pk, MD 20742 USA. [Zwick, Andreas] State Museum Nat Hist, Dept Entomol, Stuttgart, Germany. RP Regier, JC (reprint author), Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. EM cmitter@umd.edu RI Zwick, Andreas/A-5735-2015; Baixeras, Joaquin/L-1734-2014 OI Baixeras, Joaquin/0000-0002-6092-0496 FU U.S. National Science Foundation [0531626, 0531769]; Spanish Government (Ministerio de Ciencia e Innovacion) [CGL2008-00605] FX Financial support was provided by the U.S. National Science Foundation's Assembling the Tree of Life program, award numbers 0531626 and 0531769, and grant CGL2008-00605 of the Spanish Government (Ministerio de Ciencia e Innovacion) to Dr. Brown. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 103 TC 30 Z9 32 U1 0 U2 34 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 19 PY 2012 VL 7 IS 4 AR e35574 DI 10.1371/journal.pone.0035574 PG 17 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TL UT WOS:000305336200057 PM 22536410 ER PT J AU Chen, JW Faisal, SM Chandra, S McDonough, SP Moreira, MAS Scaria, J Chang, CF Bannantine, JP Akey, B Chang, YF AF Chen, Jenn-Wei Faisal, Syed M. Chandra, Subhash McDonough, Sean P. Moreira, Maria A. S. Scaria, Joy Chang, Chao-Fu Bannantine, John P. Akey, Bruce Chang, Yung-Fu TI Immunogenicity and protective efficacy of the Mycobacterium avium subsp paratuberculosis attenuated mutants against challenge in a mouse model SO VACCINE LA English DT Article DE Mycobacterium avium subsp paratuberculosis; Mutant; Johne's disease; leuD, mpt64; secA2 ID IMMUNE-RESPONSES; JOHNES-DISEASE; BOVIS BCG; VIRULENCE DETERMINANTS; RECOMBINANT ANTIGENS; AUXOTROPHIC MUTANTS; IN-VITRO; TUBERCULOSIS; VACCINE; LEUCINE AB Johne's disease (JD), caused by Mycobacterium avium subsp. paratuberculosis (MAP), results in serious economic losses worldwide especially in cattle, sheep and goats. To control the impact of JD on the animal industry, an effective vaccine with minimal adverse effects is urgently required. In order to develop an effective vaccine, we used allelic exchange to construct three mutant MAP strains, leuD, mpt64 and secA2. The mutants were attenuated in a murine model and induced cytokine responses in J774A.1 cell. The leuD mutant was the most obviously attenuated of the three constructed mutant strains. Our preliminary vaccine trial in mice demonstrated different levels of protection were induced by these mutants based on the acid-fast bacilli burden in livers and spleens at 8 and 12 weeks postchallenge. In addition, vaccination with leuD mutant induced a high level of IFN-gamma production and significant protective efficacy in both the reduction of inflammation and clearance of acid-fast bacilli, as compared with the mock vaccinated group. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Chen, Jenn-Wei; Faisal, Syed M.; Chandra, Subhash; Moreira, Maria A. S.; Scaria, Joy; Akey, Bruce; Chang, Yung-Fu] Cornell Univ, Anim Hlth Diagnost Ctr, Dept Populat Med & Diagnost Sci, Coll Vet Med, Ithaca, NY 14853 USA. [Chang, Chao-Fu] Cent Taiwan Univ Sci & Technol, Inst Med Biotechnol, Taichung, Taiwan. [Bannantine, John P.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Chang, YF (reprint author), Cornell Univ, Anim Hlth Diagnost Ctr, Dept Populat Med & Diagnost Sci, Coll Vet Med, Ithaca, NY 14853 USA. EM yc42@cornell.edu OI Bannantine, John/0000-0002-5692-7898 FU Biotechnology Research and Development Corporation (BRDC); USDA-APHIS; National Research Initiative (NRI) of the USDA Cooperative State Research, Education and Extension Service [2008-35204-04626]; Agriculture and Food Research Initiative (AFRI) of the USDA Cooperative State Research, Education and Extension Service [2009-65119-05993]; Animal Formula Fund [NY-478455, NY-478437]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil FX This work was supported by the Biotechnology Research and Development Corporation (BRDC), a contract through a cooperative agreement between the NYS Department of Agriculture and Markets, the USDA-APHIS, the National Research Initiative (NRI) of the USDA Cooperative State Research, Education and Extension Service (2008-35204-04626), the Agriculture and Food Research Initiative (AFRI) of the USDA Cooperative State Research, Education and Extension Service (2009-65119-05993) and the Animal Formula Fund (NY-478455 and NY-478437). Maria A.S. Moreira was supported by a scholarship from the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil. NR 38 TC 9 Z9 9 U1 1 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD APR 19 PY 2012 VL 30 IS 19 BP 3015 EP 3025 DI 10.1016/j.vaccine.2011.11.029 PG 11 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 934FM UT WOS:000303429100016 PM 22107851 ER PT J AU Crow, WT Berg, AA Cosh, MH Loew, A Mohanty, BP Panciera, R de Rosnay, P Ryu, D Walker, JP AF Crow, Wade T. Berg, Aaron A. Cosh, Michael H. Loew, Alexander Mohanty, Binayak P. Panciera, Rocco de Rosnay, Patricia Ryu, Dongryeol Walker, Jeffrey P. TI UPSCALING SPARSE GROUND-BASED SOIL MOISTURE OBSERVATIONS FOR THE VALIDATION OF COARSE-RESOLUTION SATELLITE SOIL MOISTURE PRODUCTS SO REVIEWS OF GEOPHYSICS LA English DT Review ID SGP97 HYDROLOGY EXPERIMENT; POLARIMETRIC SCANNING RADIOMETER; REMOTE-SENSING FOOTPRINTS; TEMPORAL STABILITY; SPATIAL VARIABILITY; WATER CONTENT; IN-SITU; C-BAND; FIELD; RETRIEVAL AB The contrast between the point-scale nature of current ground-based soil moisture instrumentation and the ground resolution (typically >10(2) km(2)) of satellites used to retrieve soil moisture poses a significant challenge for the validation of data products from current and upcoming soil moisture satellite missions. Given typical levels of observed spatial variability in soil moisture fields, this mismatch confounds mission validation goals by introducing significant sampling uncertainty in footprint-scale soil moisture estimates obtained from sparse ground-based observations. During validation activities based on comparisons between ground observations and satellite retrievals, this sampling error can be misattributed to retrieval uncertainty and spuriously degrade the perceived accuracy of satellite soil moisture products. This review paper describes the magnitude of the soil moisture upscaling problem and measurement density requirements for ground-based soil moisture networks. Since many large-scale networks do not meet these requirements, it also summarizes a number of existing soil moisture upscaling strategies which may reduce the detrimental impact of spatial sampling errors on the reliability of satellite soil moisture validation using spatially sparse ground-based observations. C1 [Crow, Wade T.; Cosh, Michael H.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Berg, Aaron A.] Univ Guelph, Dept Geog, Guelph, ON N1G 2W1, Canada. [de Rosnay, Patricia] European Ctr Medium Range Weather Forecastsing, Reading RG2 9AX, Berks, England. [Loew, Alexander] Max Planck Inst Meteorol, D-20146 Hamburg, Germany. [Mohanty, Binayak P.] Texas A&M Univ, Dept Biol & Agr Engn, College Stn, TX 77843 USA. [Panciera, Rocco; Ryu, Dongryeol] Univ Melbourne, Dept Infrastruct Engn, Melbourne, Vic 3010, Australia. [Walker, Jeffrey P.] Monash Univ, Dept Civil Engn, Clayton, Vic 3800, Australia. RP Crow, WT (reprint author), ARS, Hydrol & Remote Sensing Lab, USDA, Rm 104,Bldg 007,BARC W, Beltsville, MD 20705 USA. EM wade.crow@ars.usda.gov RI Walker, Jeffrey/D-2624-2009; de Rosnay, Patricia/M-8203-2013; Cosh, MIchael/A-8858-2015; Ryu, Dongryeol/C-5903-2008 OI de Rosnay, Patricia/0000-0002-7374-3820; Cosh, MIchael/0000-0003-4776-1918; Ryu, Dongryeol/0000-0002-5335-6209 FU SMAP Science Definition Team FX Research for this paper was directed in concert with the NASA Soil Moisture Active/Passive (SMAP) Working Group on Validation and supported by funding from the SMAP Science Definition Team. NR 112 TC 54 Z9 56 U1 3 U2 58 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 8755-1209 EI 1944-9208 J9 REV GEOPHYS JI Rev. Geophys. PD APR 19 PY 2012 VL 50 AR RG2002 DI 10.1029/2011RG000372 PG 20 WC Geochemistry & Geophysics SC Geochemistry & Geophysics GA 930GA UT WOS:000303124500001 ER PT J AU Kasuga, T Kozanitas, M Bui, M Huberli, D Rizzo, DM Garbelotto, M AF Kasuga, Takao Kozanitas, Melina Bui, Mai Hueberli, Daniel Rizzo, David M. Garbelotto, Matteo TI Phenotypic Diversification Is Associated with Host-Induced Transposon Derepression in the Sudden Oak Death Pathogen Phytophthora ramorum SO PLOS ONE LA English DT Article ID 3 CLONAL LINEAGES; POPULATION-STRUCTURE; GENOME SEQUENCE; MICROARRAY DATA; GENE; CALIFORNIA; EVOLUTION; INFESTANS; FOREST; TRANSMISSION AB The oomycete pathogen Phytophthora ramorum is responsible for sudden oak death (SOD) in California coastal forests. P. ramorum is a generalist pathogen with over 100 known host species. Three or four closely related genotypes of P. ramorum (from a single lineage) were originally introduced in California forests and the pathogen reproduces clonally. Because of this the genetic diversity of P. ramorum is extremely low in Californian forests. However, P. ramorum shows diverse phenotypic variation in colony morphology, colony senescence, and virulence. In this study, we show that phenotypic variation among isolates is associated with the host species from which the microbe was originally cultured. Microarray global mRNA profiling detected derepression of transposable elements (TEs) and down-regulation of crinkler effector homologs (CRNs) in the majority of isolates originating from coast live oak (Quercus agrifolia), but this expression pattern was not observed in isolates from California bay laurel (Umbellularia californica). In some instances, oak and bay laurel isolates originating from the same geographic location had identical genotypes based on multilocus simples sequence repeat (SSR) marker analysis but had different phenotypes. Expression levels of the two marker genes analyzed by quantitative reverse transcription PCR were correlated with originating host species, but not with multilocus genotypes. Because oak is a nontransmissive dead-end host for P. ramorum, our observations are congruent with an epi-transposon hypothesis; that is, physiological stress is triggered on P. ramorum while colonizing oak stems and disrupts epigenetic silencing of TEs. This then results in TE reactivation and possibly genome diversification without significant epidemiological consequences. We propose the P. ramorum-oak host system in California forests as an ad hoc model for epi-transposon mediated diversification. C1 [Kasuga, Takao; Bui, Mai] USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA USA. [Kozanitas, Melina; Hueberli, Daniel; Garbelotto, Matteo] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Rizzo, David M.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. RP Kasuga, T (reprint author), USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA USA. EM matteog@berkeley.edu RI Huberli, Daniel/C-7257-2013 FU National Science Foundation (NSF); National Institutes of Health (NIH) Ecology of Infectious Diseases [EF-0622770, 1115607]; Gordon and Betty Moore Foundation; Pacific Southwest Research Station, the United States Department of Agriculture (USDA) Forest Service; USDA Agricultural Research Service CRIS Project [5306-22000-014-00D] FX This work was supported by the National Science Foundation (NSF) and the National Institutes of Health (NIH) Ecology of Infectious Diseases # EF-0622770 (to DMR) and # 1115607 (to MG); The Gordon and Betty Moore Foundation (to DMR and to MG); the Pacific Southwest Research Station, the United States Department of Agriculture (USDA) Forest Service (to MG); and USDA Agricultural Research Service CRIS Project # 5306-22000-014-00D (to TK). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 69 TC 26 Z9 26 U1 0 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 18 PY 2012 VL 7 IS 4 AR e34728 DI 10.1371/journal.pone.0034728 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959XD UT WOS:000305350600023 PM 22529930 ER PT J AU Payn, RA Gooseff, MN McGlynn, BL Bencala, KE Wondzell, SM AF Payn, R. A. Gooseff, M. N. McGlynn, B. L. Bencala, K. E. Wondzell, S. M. TI Exploring changes in the spatial distribution of stream baseflow generation during a seasonal recession SO WATER RESOURCES RESEARCH LA English DT Article ID HYPORHEIC EXCHANGE; GROUNDWATER-FLOW; CATCHMENT-SCALE; RESIDENCE TIME; VARIABILITY; TOPOGRAPHY; HYDROLOGY; GEOMORPHOLOGY; CONNECTIVITY; MOUNTAINS AB Relating watershed structure to streamflow generation is a primary focus of hydrology. However, comparisons of longitudinal variability in stream discharge with adjacent valley structure have been rare, resulting in poor understanding of the distribution of the hydrologic mechanisms that cause variability in streamflow generation along valleys. This study explores detailed surveys of stream base flow across a gauged, 23 km(2) mountain watershed. Research objectives were (1) to relate spatial variability in base flow to fundamental elements of watershed structure, primarily topographic contributing area, and (2) to assess temporal changes in the spatial patterns of those relationships during a seasonal base flow recession. We analyzed spatiotemporal variability in base flow using (1) summer hydrographs at the study watershed outlet and 5 subwatershed outlets and (2) longitudinal series of discharge measurements every similar to 100 m along the streams of the 3 largest subwatersheds (1200 to 2600 m in valley length), repeated 2 to 3 times during base flow recession. Reaches within valley segments of 300 to 1200 m in length tended to demonstrate similar streamflow generation characteristics. Locations of transitions between these segments were consistent throughout the recession, and tended to be collocated with abrupt longitudinal transitions in valley slope or hillslope-riparian characteristics. Both within and among subwatersheds, correlation between the spatial distributions of streamflow and topographic contributing area decreased during the recession, suggesting a general decrease in the influence of topography on stream base flow contributions. As topographic controls on base flow evidently decreased, multiple aspects of subsurface structure were likely to have gained influence. C1 [Payn, R. A.] Colorado Sch Mines, Dept Geol & Geol Engn, Hydrol Sci & Engn Program, Golden, CO 80401 USA. [Payn, R. A.; McGlynn, B. L.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Bencala, K. E.] US Geol Survey, Menlo Pk, CA 94025 USA. [Gooseff, M. N.] Penn State Univ, Dept Civil & Environm Engn, University Pk, PA 16802 USA. [Wondzell, S. M.] ARS, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP Payn, RA (reprint author), Colorado Sch Mines, Dept Geol & Geol Engn, Hydrol Sci & Engn Program, Golden, CO 80401 USA. EM rpayn@montana.edu RI McGlynn, Brian/A-2509-2008; Gooseff, Michael/N-6087-2015 OI McGlynn, Brian/0000-0001-5266-4894; Gooseff, Michael/0000-0003-4322-8315 FU NSF [EAR 03-37650, EAR 05-30873] FX We thank Kelsey Jencso, Austin Allen, Aurora Bouchier, and Martin Briggs for assistance in the field. We thank the anonymous reviewers who have taken the time for extensive comments, greatly improving the clarity of this paper. We also thank the Tenderfoot Creek Experimental Forest and the U.S. Department of Agriculture, especially Ward McCaughey. This research was supported by collaborative NSF grants EAR 03-37650 to BLM and EAR 05-30873 to MNG. The findings and opinions reported here do not necessarily reflect those of the National Science Foundation. NR 44 TC 33 Z9 33 U1 4 U2 41 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD APR 18 PY 2012 VL 48 AR W04519 DI 10.1029/2011WR011552 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 930GC UT WOS:000303124700003 ER PT J AU Jones, AMP Klun, JA Cantrell, CL Ragone, D Chauhan, KR Brown, PN Murch, SJ AF Jones, A. Maxwell P. Klun, Jerome A. Cantrell, Charles L. Ragone, Diane Chauhan, Kamlesh R. Brown, Paula N. Murch, Susan J. TI Isolation and Identification of Mosquito (Aedes aegypti) Biting Deterrent Fatty Acids from Male Inflorescences of Breadfruit (Artocarpus altilis (Parkinson) Fosberg) SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE mosquito repellent; capric acid; undecanoic acid; hendecanoic acids; lauric acid; hydrodistillation; smoke AB Dried male inflorescences of breadfruit (Artocarpus altilis, Moraceae) are burned in communities throughout Oceania to repel flying insects, including mosquitoes. This study was conducted to identify chemicals responsible for mosquito deterrence. Various crude extracts were evaluated, and the most active, the hydrodistillate, was used for bioassay-guided fractionation. The hydrodistillate and all fractions displayed significant deterrent activity. Exploratory GC-MS analysis revealed more than 100 distinctive peaks, and more than 30 compounds were putatively identified, including a mixture of terpenes, aldehydes, fatty acids, and aromatics. A systematic bioassay-directed study using adult Aedes aegypti females identified capric, undecanoic, and lauric acid as primary deterrent constituents. A synthetic mixture of fatty acids present in the most active fraction and individual fatty acids were all significantly more active than N,N-diethyl-m-toluamide (DEET). These results provide support for this traditional practice and indicate the potential of male breadfruit flowers and fatty acids as mosquito repellents. C1 [Jones, A. Maxwell P.; Brown, Paula N.; Murch, Susan J.] Univ British Columbia, Kelowna, BC V1V 1V7, Canada. [Klun, Jerome A.; Cantrell, Charles L.; Chauhan, Kamlesh R.] ARS, USDA, Beltsville, MD 20705 USA. [Cantrell, Charles L.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Ragone, Diane] Natl Trop Bot Garden, Breadfruit Inst, Kalaheo, HI 96741 USA. [Brown, Paula N.] British Columbia Inst Technol, Burnaby, BC V5G 3H2, Canada. RP Jones, AMP (reprint author), Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. EM amjones@uoguelph.ca FU Board of Trustees and Fellows at the National Tropical Botanical Garden FX We acknowledge the generosity of the Board of Trustees and Fellows at the National Tropical Botanical Garden for their ongoing support of the Breadfruit Institute's research. NR 14 TC 15 Z9 15 U1 3 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 18 PY 2012 VL 60 IS 15 BP 3867 EP 3873 DI 10.1021/jf300101w PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 927DL UT WOS:000302887100008 PM 22420541 ER PT J AU Choi, SH Ahn, JB Kozukue, N Kim, HJ Nishitani, Y Zhang, L Mizuno, M Levin, CE Friedman, M AF Choi, Suk Hyun Ahn, Jun-Bae Kozukue, Nobuyuki Kim, Hyun-Jeong Nishitani, Yosuke Zhang, Ling Mizuno, Masashi Levin, Carol E. Friedman, Mendel TI Structure-Activity Relationships of alpha-, beta(1)-, gamma-, and delta-Tomatine and Tomatidine against Human Breast (MDA-MB-231), Gastric (KATO-III), and Prostate (PC3) Cancer Cells SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Tomatoes; alpha-tomatine; hydrolysis; TLC; mass spectrometry; cancer cells; inhibition; tumor necrosis factor alpha; structure-function relationships; mechanisms ID LYCOPERSICON-ESCULENTUM; GLYCOALKALOID TOMATINE; STEROIDAL ALKALOIDS; INDUCED NECROPTOSIS; SOLANUM-TUBEROSUM; L929 CELLS; LIVER; PLANT; CHOLESTEROL; POTATOES AB Partial acid hydrolysis of the tetrasaccharide (lycotetraose) side chain of the tomato glycoalkaloid alpha-tomatine resulted in the formation of four products with three, two, one, and zero carbohydrate side chains, which were separated by high-performance liquid chromatography (HPLC) and identified by thin-layer chromatography (TLC) and liquid chromatography ion-trap time-of-flight mass spectrometry (LCMS-IT-TOF). The inhibitory activities in terms of IC50 values (concentration that inhibits 50% of the cells under the test conditions) of the parent compound and the hydrolysates, isolated by preparative HPLC, against normal human liver and lung cells and human breast, gastric, and prostate cancer cells indicate that (a) the removal of sugars significantly reduced the concentration-dependent cell-inhibiting effects of the test compounds, (b) PC3 prostate cancer cells were about 10 times more susceptible to inhibition by alpha-tomatine than the breast and gastric cancer cells or the normal cells, (c) the activity of alpha-tomatine against the prostate cancer cells was 200 times greater than that of the aglycone tomatidine, and (d) the activity increased as the number of sugars on the aglycone increased, but this was only statistically significant at p < 0.05 for the normal lung He1299 cell line. The effect of the alkaloids on tumor necrosis factor alpha (TNF-alpha) was measured in RAW264.7 macrophage cells. There was a statistically significant negative correlation between the dosage of gamma- and alpha-tomatine and the level of TNF-alpha. alpha-Tomatine was the most effective compound at reducing TNF-alpha. The dietary significance of the results and future research needs are discussed. C1 [Levin, Carol E.; Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Choi, Suk Hyun; Ahn, Jun-Bae] Seowon Univ, Dept Food Serv Ind, Cheongju 361742, South Korea. [Kozukue, Nobuyuki] Seowon Univ, Bio Organ Mat & Food Ctr, Cheongju 361742, South Korea. [Kim, Hyun-Jeong] Keimyung Univ, Ctr Tradit Microorganism Resources, Taegu 704701, South Korea. [Nishitani, Yosuke] Kobe Univ, Org Adv Sci & Technol, Team Hlth Biosci, Kobe, Hyogo 6578501, Japan. [Zhang, Ling; Mizuno, Masashi] Kobe Univ, Dept Agrobiosci, Grad Sch Agr Sci, Kobe, Hyogo 6578501, Japan. RP Friedman, M (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM mendel.friedman@ars.usda.gov OI Levin, Carol/0000-0001-6522-6156 NR 55 TC 13 Z9 14 U1 1 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 18 PY 2012 VL 60 IS 15 BP 3891 EP 3899 DI 10.1021/jf3003027 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 927DL UT WOS:000302887100011 PM 22482398 ER PT J AU Fabrick, JA Tabashnik, BE AF Fabrick, Jeffrey A. Tabashnik, Bruce E. TI Similar Genetic Basis of Resistance to Bt Toxin Cry1Ac in Boll-Selected and Diet-Selected Strains of Pink Bollworm SO PLOS ONE LA English DT Article ID BACILLUS-THURINGIENSIS TOXINS; PECTINOPHORA-GOSSYPIELLA; INSECT RESISTANCE; TRANSGENIC COTTON; FITNESS COSTS; HELICOVERPA-ARMIGERA; HELIOTHIS-VIRESCENS; CROSS-RESISTANCE; DIAMONDBACK MOTH; CROPS AB Genetically engineered cotton and corn plants producing insecticidal Bacillus thuringiensis (Bt) toxins kill some key insect pests. Yet, evolution of resistance by pests threatens long-term insect control by these transgenic Bt crops. We compared the genetic basis of resistance to Bt toxin Cry1Ac in two independently derived, laboratory-selected strains of a major cotton pest, the pink bollworm (Pectinophora gossypiella [Saunders]). The Arizona pooled resistant strain (AZP-R) was started with pink bollworm from 10 field populations and selected with Cry1Ac in diet. The Bt4R resistant strain was started with a long-term susceptible laboratory strain and selected first with Bt cotton bolls and later with Cry1Ac in diet. Previous work showed that AZP-R had three recessive mutations (r1, r2, and r3) in the pink bollworm cadherin gene (PgCad1) linked with resistance to Cry1Ac and Bt cotton producing Cry1Ac. Here we report that inheritance of resistance to a diagnostic concentration of Cry1Ac was recessive in Bt4R. In interstrain complementation tests for allelism, F-1 progeny from crosses between AZP-R and Bt4R were resistant to Cry1Ac, indicating a shared resistance locus in the two strains. Molecular analysis of the Bt4R cadherin gene identified a novel 15-bp deletion (r4) predicted to cause the loss of five amino acids upstream of the Cry1Ac-binding region of the cadherin protein. Four recessive mutations in PgCad1 are now implicated in resistance in five different strains, showing that mutations in cadherin are the primary mechanism of resistance to Cry1Ac in laboratory-selected strains of pink bollworm from Arizona. C1 [Fabrick, Jeffrey A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ USA. [Tabashnik, Bruce E.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. RP Fabrick, JA (reprint author), USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ USA. EM jeff.fabrick@ars.usda.gov FU USDA-Agriculture and Food Research Initiative [2008-35302-0390] FX This work was supported by USDA-Agriculture and Food Research Initiative Grant 2008-35302-0390. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 16 Z9 16 U1 1 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 18 PY 2012 VL 7 IS 4 AR e35658 DI 10.1371/journal.pone.0035658 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959XD UT WOS:000305350600111 PM 22530065 ER PT J AU Shields, FD Alonso, CV AF Shields, F. Douglas, Jr. Alonso, Carlos V. TI Assessment of flow forces on large wood in rivers SO WATER RESOURCES RESEARCH LA English DT Article ID SAND-BED STREAMS; HABITAT REHABILITATION; DEBRIS; RESISTANCE; FLUME; CHANNELS; SCOUR; LOGS; MISSISSIPPI; MORPHOLOGY AB Large wood (LW) exerts an important influence on the geomorphology and ecology of streams and rivers. The magnitudes of flow forces on LW are needed to support stream management activities and are typically computed using time mean lift and drag coefficients determined in laboratory flumes using small, smooth cylinders. Herein we report measurements of forces on LW of varying complexity (simple cylinder, branching, and complex root wad) and surface (bark) roughness made in an outdoor grassed channel under steady and unsteady flows. LW orientation relative to the primary flow direction and LW relative submergence were varied. Drag and lift coefficients for cylindrical (unbranched) LW followed patterns reported by others for metal cylinders in wind tunnels. Drag coefficients for cylindrical (unbranched) LW, corrected for blockage effects, ranged from -0.05 to 1.29, and lift coefficients ranged from -0.88 to 0.52, varying systematically with LW position relative to the channel bed and incident flow direction. Measured drag coefficients for the noncylindrical LW, corrected for blockage effects, ranged from 0.22 to 6.27, while lift coefficients varied from -3.65 to 30.84. Systematic relationships between the relative submergence and orientation of branching LW and the drag and lift coefficients were not observed, but coefficients were greatest for LW with few branches and converged on smaller values typical of blunt bodies as LW complexity increased. For both simple and complex LW, maximum lift and drag forces during the rising limb of unsteady flows were about 2-3 times greater than steady flow temporal mean values. C1 [Shields, F. Douglas, Jr.; Alonso, Carlos V.] ARS, Natl Sedimentat Lab, USDA, Oxford, MS 38655 USA. RP Shields, FD (reprint author), ARS, Natl Sedimentat Lab, USDA, POB 1157,598 McElroy Dr,MS 38655, Oxford, MS 38655 USA. EM doug.shields@ars.usda.gov NR 74 TC 10 Z9 10 U1 2 U2 19 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 J9 WATER RESOUR RES JI Water Resour. Res. PD APR 17 PY 2012 VL 48 AR W04516 DI 10.1029/2011WR011547 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 930FY UT WOS:000303124300002 ER PT J AU Sun, XB Chen, P Cook, SL Jackson, GP Harnly, JM Harrington, PB AF Sun, Xiaobo Chen, Pei Cook, Shannon L. Jackson, Glen P. Harnly, James M. Harrington, Peter B. TI Classification of Cultivation Locations of Panax quinquefolius L Samples using High Performance Liquid Chromatography - Electrospray Ionization Mass Spectrometry and Chemometric Analysis SO ANALYTICAL CHEMISTRY LA English DT Article ID BUILDING EXPERT-SYSTEMS; AMERICAN-GINSENG; GAS-CHROMATOGRAPHY; INFRARED-SPECTROSCOPY; MOBILITY SPECTROMETRY; RAMAN-SPECTROSCOPY; QUALITY-CONTROL; ASIAN GINSENG; GINSENOSIDES; DIFFERENTIATION AB Panax quinquefolius L (P. quinquefolius L) samples grown in the United States and China were analyzed with high performance liquid chromatography-mass spectrometry (HPLC MS). Prior to classification, the two-way data sets were subjected to pretreatment including baseline correction and retention time (RT) alignment. Principal component analysis (PCA) and projected difference resolution (PDR) metrics were used to evaluate the data quality and the pretreatment effects. A fuzzy rule-building expert system (FuRES) classifier was used to classify the P. quinquefolius L samples grown in the United States and China with the optimized partial least-squares (o-PLS) classifier as the positively biased control method. A classification rate as high as 98 +/- 3% with FuRES was obtained after baseline correction and RT alignment, which is equivalent to the result obtained by using the positively biased o-PLS control method (98 +/- 3%). RT alignment improved the classification rates for both FuRES and o-PLS classifiers (18% improvement for the FuRES classification rate and 10% improvement for the o-PLS classification rate with baseline correction). From the rule obtained to classify the P. quinquefolius L samples grown in the United States and China, peaks were identified that can be prospective biomarkers for differentiating samples from different growth regions. HPLC MS with chemometric analysis has the potential to be used as an authentication method for P. quinquefolius L grown in China and the United States. C1 [Sun, Xiaobo; Cook, Shannon L.; Jackson, Glen P.; Harrington, Peter B.] Ohio Univ, Dept Chem & Biochem, Clippinger Labs, Ctr Intelligent Chem Instrumentat, Athens, OH 45701 USA. [Chen, Pei; Harnly, James M.] ARS, Food Composit Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Harrington, PB (reprint author), Ohio Univ, Dept Chem & Biochem, Clippinger Labs, Ctr Intelligent Chem Instrumentat, Athens, OH 45701 USA. EM peter.harrington@ohio.edu RI Jackson, Glen/D-3518-2014; OI Jackson, Glen/0000-0003-0803-6254; Harrington, Peter/0000-0003-0268-8630 FU NSF [CHE 0745590] FX Weiying Lu, Mengliang Zhang, Zhanfeng Xu, and Zhengfang Wang are thanked for their help and suggestions. Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, is thanked for providing the ginseng samples. G.P.J. acknowledges financial support from NSF grant CHE 0745590. NR 45 TC 11 Z9 12 U1 3 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 J9 ANAL CHEM JI Anal. Chem. PD APR 17 PY 2012 VL 84 IS 8 BP 3628 EP 3634 DI 10.1021/ac2032832 PG 7 WC Chemistry, Analytical SC Chemistry GA 926NQ UT WOS:000302838500022 PM 22414002 ER PT J AU Covino, T McGlynn, B McNamara, R AF Covino, Tim McGlynn, Brian McNamara, Rebecca TI Land use/land cover and scale influences on in-stream nitrogen uptake kinetics SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article ID NUTRIENT-UPTAKE; UPTAKE LENGTH; SATURATION; DYNAMICS; IMPACTS AB Land use/land cover change often leads to increased nutrient loading to streams; however, its influence on stream ecosystem nutrient transport remains poorly understood. Given the deleterious impacts elevated nutrient loading can have on aquatic ecosystems, it is imperative to improve understanding of nutrient retention capacities across stream scales and watershed development gradients. We performed 17 nutrient addition experiments on six streams across the West Fork Gallatin Watershed, Montana, USA, to quantify nitrogen uptake kinetics and retention dynamics across stream sizes (first to fourth order) and along a watershed development gradient. We observed that stream nitrogen (N) uptake kinetics and spiraling parameters varied across streams of different development intensity and scale. In more developed watersheds we observed a fertilization affect. This fertilization affect was evident as increased ash-free dry mass, chlorophyll a, and ambient and maximum uptake rates in developed as compared to undeveloped streams. Ash-free dry mass, chlorophyll a, and the number of structures in a subwatershed were significantly correlated to nutrient spiraling and kinetic parameters, while ambient and average annual N concentrations were not. Additionally, increased maximum uptake capacities in developed streams contributed to low in-stream nutrient concentrations during the growing season, and helped maintain watershed export at low levels during base flow. Our results indicate that land use/land cover change can enhance in-stream uptake of limiting nutrients and highlight the need for improved understanding of the watershed dynamics that control nutrient export across scales and development intensities for mitigation and protection of aquatic ecosystems. C1 [Covino, Tim; McGlynn, Brian; McNamara, Rebecca] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [McNamara, Rebecca] US Forest Serv, Butte, MT USA. RP Covino, T (reprint author), Montana State Univ, Dept Land Resources & Environm Sci, 334 Leon Johnson Hall, Bozeman, MT 59717 USA. EM tpcovino@gmail.com RI McGlynn, Brian/A-2509-2008 OI McGlynn, Brian/0000-0001-5266-4894 FU National Science Foundation (NSF) Ecosystems [DEB-0519264]; NSF EPSCoR Montana Institute; Environmental Protection Agency (EPA) STAR [R832449] FX Financial support was provided by National Science Foundation (NSF) Ecosystems DEB-0519264, NSF EPSCoR Montana Institute on Ecosystems Fellowship awarded to Covino, an Environmental Protection Agency (EPA) STAR Fellowship awarded to Covino, EPA STAR grant R832449, EPA 319 funds administered by the Montana Department of Environmental Quality, and the USGS 104(b) grant program administered by the Montana Water Center. We thank Leslie Piper for ash-free dry mass and chlorophyll a data, Galena Ackerman and John Mallard for laboratory analysis, and Tricia Jenkins for help collecting field samples. We thank the Big Sky community for allowing access to sampling sites. NR 27 TC 10 Z9 11 U1 2 U2 37 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0148-0227 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD APR 17 PY 2012 VL 117 AR G02006 DI 10.1029/2011JG001874 PG 13 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 930ES UT WOS:000303120800001 ER PT J AU Chen, WX Jin, TZ Gurtler, JB Geveke, DJ Fan, XT AF Chen, Wenxuan Jin, Tony Z. Gurtler, Joshua B. Geveke, David J. Fan, Xuetong TI Inactivation of Salmonella on whole cantaloupe by application of an antimicrobial coating containing chitosan and allyl isothiocyanate SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Cantaloupe; Salmonella; Antimicrobial coating; Chitosan; Allyl isothiocyanate ID ESCHERICHIA-COLI O157-H7; LISTERIA-MONOCYTOGENES; POLYLACTIC ACID; FOOD PRESERVATION; OUTER-MEMBRANE; ACETIC-ACID; NISIN; BACTERIA; SURFACE; FILMS AB This study investigated the antimicrobial effect of a chitosan coating + allyl isothiocyanate (AIT) and nisin against Salmonella on whole fresh cantaloupes. Cantaloupes were inoculated with a cocktail of three Salmonella strains and treated with chitosan, chitosan + AIT, chitosan + nisin, and chitosan + AIT + nisin coatings. With AIT concentrations increasing from 10 to 60 mu l/ml, the antibacterial effects of coating treatments against Salmonella increased. Chitosan coatings with 60 mu l/ml AIT (chitosan + 60AIT) reduced more than 5 log(10) CFU/cm(2) of Salmonella. The addition of nisin to the chitosan-AIT coating synergistically increased the antibacterial effect; coatings with nisin (25 mg/ml or 25,000 IU/ml) + 30 mu l/ml AIT resulted in a 4.8 log(10) reduction of Salmonella. The chitosan + 60AIT coating significantly (p < 0.05) reduced populations of native bacteria on cantaloupes to ca. 2 log(10) CFU/cm(2) during the first 6 days and populations remained unchanged through day 14 at 10 degrees C. The same coating treatment completely inactivated mold and yeast on cantaloupe at day 1 and no regrowth occurred even up to 14 days of storage. Scanning electron microscopy revealed that cell membrane damage and leakage of intercellular components occurred as a result of the chitosan-AIT coating treatments. No visual changes in overall appearance and color of cantaloupe rind and flesh due to coating treatments were observed. These results indicate that the application of an antimicrobial coating may be an effective method for decontamination of cantaloupes. Published by Elsevier B.V. C1 [Jin, Tony Z.; Gurtler, Joshua B.; Geveke, David J.; Fan, Xuetong] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Chen, Wenxuan] Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China. RP Jin, TZ (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ari.usda.gov OI Jin, Tony/0000-0003-0504-5817 FU USDA; ARS CRIS [1935-41420-092-00D] FX This study was funded by the USDA, the ARS CRIS project 1935-41420-092-00D through ARS National Program 108. The authors wish to thank Anita Parameswaran, Rebecca Rivera, Gaoping Bao, and Andy Bigley for their technical assistance. NR 55 TC 28 Z9 32 U1 2 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD APR 16 PY 2012 VL 155 IS 3 BP 165 EP 170 DI 10.1016/j.ijfoodmicro.2012.02.001 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA 932KJ UT WOS:000303289700010 PM 22361025 ER PT J AU Dancho, BA Chen, HQ Kingsley, DH AF Dancho, Brooke A. Chen, Haiqiang Kingsley, David H. TI Discrimination between infectious and non-infectious human norovirus using porcine gastric mucin SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Human norovirus; Porcine mucin-conjugated magnetic beads; UV inactivation; High pressure processing; Thermal inactivation ID BLOOD GROUP ANTIGEN; HIGH HYDROSTATIC-PRESSURE; TIME RT-PCR; NORWALK VIRUS; MURINE NOROVIRUS-1; FELINE CALICIVIRUS; ENTERIC VIRUSES; MAGNETIC BEADS; INACTIVATION; BINDING AB Human noroviruses (NoVs) are known to bind to human histo-blood group antigens, as well as to chemically-similar porcine gastric mucins. Here, the binding ability of NoV to porcine mucin is shown to be substantially deficient after UV, thermal, and high pressure treatments. Using qRT-PCR, >= 68% of GI.1 NoV (Norwalk strain) bound to porcine gastric mucin-conjugated magnetic beads (PGM-MBs). Application of 600-MPa high pressure treatments reduced binding of the virus to PGM-MBs by 4.7-log(10), as determined by qRT-PCR, while a 300-MPa pressure treatment, reduced binding to PGM-MBs by only 0.45-log(10). This is consistent with a previously reported clinical trial (Leon et at, 2011. Appl. Environ Microbiol. 77:5476-5482.) which demonstrated inactivation of 4-log(10) of GI.1 NoV at 600-MPa. After thermal treatment, binding to PGM-MBs decreased when samples were heated from 0 to 80 degrees C. Ultraviolet treatments of 0.5 and 2 J/cm(2) reduced observed PGM-MB binding of norovirus to 33% and negligible levels, respectively, from an initially observed 84% binding for untreated NoV. Although thermal and UV treatments are generally recognized to inactivate viruses, verification of NoV inactivation by these treatments may require volunteer studies. In total, these results suggest the loss of NoV binding to porcine mucin as a potential means to preferentially exclude non-infectious virus particles from subsequent RT-PCR detection. Published by Elsevier B.V. C1 [Kingsley, David H.] Delaware State Univ, USDA, ARS, James WW Baker Ctr,Food Safety & Intervent Techno, Dover, DE 19901 USA. [Chen, Haiqiang] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. RP Kingsley, DH (reprint author), Delaware State Univ, USDA, ARS, James WW Baker Ctr,Food Safety & Intervent Techno, Dover, DE 19901 USA. EM David.Kingsley@ars.usda.gov NR 27 TC 47 Z9 47 U1 7 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD APR 16 PY 2012 VL 155 IS 3 BP 222 EP 226 DI 10.1016/j.ijfoodmicro.2012.02.010 PG 5 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA 932KJ UT WOS:000303289700018 PM 22425335 ER PT J AU Straus, DL Meinelt, T Farmer, BD Beck, BH AF Straus, David L. Meinelt, Thomas Farmer, Bradley D. Beck, Benjamin H. TI Acute toxicity and histopathology of channel catfish fry exposed to peracetic acid SO AQUACULTURE LA English DT Article DE Peracetic acid; Acute toxicity; Histopathology; Channel catfish fry ID ICHTHYOPHTHIRIUS-MULTIFILIIS; HATCHERY MANAGEMENT; PAA; DISINFECTION; THERONTS; WATER; EGGS AB Peracetic acid (PAA) can potentially be used to treat against freshwater fish pathogens; however, there is a paucity of data about its toxicity to fish. Channel catfish Ictalurus punctatus yolk-sac fry and swim-up fry were exposed to PM for 48 h in static toxicity bioassays at 23 degrees C. The test water was 217 and 126 mg/L (as CaCO3) total alkalinity and total hardness, respectively. Probit LC50 values were estimated with the trimmed Spearman-Karber method using nominal PAA concentrations (1.0, 1.3, 1.7, 2.2, 2.8, 3.7, 4.8, 6.3, and 8.2 mg/L PM). The mean 24- and 48-h LC50 values for yolk-sac fry and swim-up fry was 2.6 mg/L PM and 1.6 mg/L PM, respectively; the 24-h no observed effect concentration (NOEC) was 2.2 mg/L PM for yolk-sac fry and 1.3 mg/L PM for swim-up fly. Catfish yolk-sac fry were more tolerant of PM than catfish swim-up fry by about 1.6-fold. Peracetic acid-related histopathology was analyzed in whole-body serial sections of swim-up fry at two selected doses of PM: 2.2 mg/L (1 h post-treatment) and 1.7 mg/L (48 h post-treatment). Lesions consisted of severe gill damage in fish treated with 2.2 mg/L PM, and a moderate degeneration of the renal tubule epithelium within the posterior kidney in fish treated with 1.7 mg/L PM. An advantage to using PM includes very low environmental impact considerations as it degrades to harmless residues rapidly. Results of the present study are important information needed by the aquaculture industry on a new, potential therapeutant. Published by Elsevier B.V. C1 [Straus, David L.; Farmer, Bradley D.; Beck, Benjamin H.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Meinelt, Thomas] Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecophysiol & Aquaculture, Berlin, Germany. RP Straus, DL (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Box 1050, Stuttgart, AR 72160 USA. EM dave.straus@ars.usda.gov NR 39 TC 9 Z9 9 U1 3 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD APR 15 PY 2012 VL 342 BP 134 EP 138 DI 10.1016/j.aquaculture.2012.02.024 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 932LV UT WOS:000303293500021 ER PT J AU Oh, MH Kim, HS Wu, X Clouse, SD Zielinski, RE Huber, SC AF Oh, Man-Ho Kim, Hyoung Seok Wu, Xia Clouse, Steven D. Zielinski, Raymond E. Huber, Steven C. TI Calcium/calmodulin inhibition of the Arabidopsis BRASSINOSTEROID-INSENSITIVE 1 receptor kinase provides a possible link between calcium and brassinosteroid signalling SO BIOCHEMICAL JOURNAL LA English DT Article DE brassinosteroid; BRASSINOSTEROID-INSENSITIVE 1 receptor kinase; calcium signalling; signal transduction; transphosphorylation; tyrosine autophosphorylation ID CALMODULIN-BINDING PROTEINS; PHOSPHOPROTEOME ANALYSIS; GENE FAMILY; PHOSPHORYLATION; TRANSDUCTION; RECOGNITION; RESIDUES; PLANTS; STRESS; BRI1 AB The receptor kinase BRI1 (BRASSINOSTEROID-INSENSITIVE 1) is a key component in BR (brassinosteroid) perception and signal transduction, and has a broad impact on plant growth and development. In the present study, we demonstrate that Arabidopsis CaM (calmodulin) binds to the recombinant cytoplasmic domain of BRI1 in a Ca2+-dependent manner in vitro. In silico analysis predicted binding to Helix E of the BRI1 kinase subdomain Via and a synthetic peptide based on this sequence interacted with Ca2+/CaM. Co-expression of CaM with the cytoplasmic domain of BRI1 in Escherichia coli strongly reduced autophosphorylation of BRI1, in particular on tyrosine residues, and also reduced the BRI1-mediated transphosphorylation of E. coli proteins on tyrosine, threonine and presumably serine residues. Several isoforms of CaM and CMLs (CaM-like proteins) were more effective (AtCaM6, AtCaM7 and AtCML8, where At is Arabidopsis thaliana) than others (AtCaM2, AtCaM4 and AtCML11) when co-expressed with BRI1 in E. coli. These results establish a novel assay for recombinant BRI1 transphosphorylation activity and collectively uncover a possible new link between Ca2+ and BR signalling. C1 [Oh, Man-Ho; Kim, Hyoung Seok; Wu, Xia; Zielinski, Raymond E.; Huber, Steven C.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Huber, Steven C.] Agr Res Serv, USDA, Urbana, IL 61801 USA. [Clouse, Steven D.] NC State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. RP Huber, SC (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM schuber1@illinois.edu RI Wu, Xia/J-8690-2014 OI Wu, Xia/0000-0002-0024-4481 FU US Department of Agriculture (USDA)-Agricultural Research Service (ARS); National Science Foundation (NSF) [IOS-1022177, MCB-1021363] FX This work was supported in part by the US Department of Agriculture (USDA)-Agricultural Research Service (ARS) and the National Science Foundation (NSF) [grant numbers IOS-1022177 and MCB-1021363]. Mention of a trademark or proprietary product does not constitute a guarantee or warranty by the USDA-ARS and does not imply its approval to the exclusion of other products that may also be suitable. Any opinions, findings, and conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the NSF or USDA-ARS. NR 48 TC 26 Z9 29 U1 2 U2 25 PU PORTLAND PRESS LTD PI LONDON PA THIRD FLOOR, EAGLE HOUSE, 16 PROCTER STREET, LONDON WC1V 6 NX, ENGLAND SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD APR 15 PY 2012 VL 443 BP 515 EP 523 DI 10.1042/BJ20111871 PN 2 PG 9 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 929WO UT WOS:000303096600019 PM 22309147 ER PT J AU Hanson, JJ Lorimer, CG Halpin, CR Palik, BJ AF Hanson, Jacob J. Lorimer, Craig G. Halpin, Corey R. Palik, Brian J. TI Ecological forestry in an uneven-aged, late-successional forest: Simulated effects of contrasting treatments on structure and yield SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Alternative forestry; Coarse woody debris; Multi-cohort management; Old-growth restoration; Reserve trees; Uneven-aged management ID NORTHERN HARDWOOD FORESTS; COARSE WOODY DEBRIS; SELF-THINNING RULE; UPPER GREAT-LAKES; OLD-GROWTH; CANOPY RECRUITMENT; PACIFIC-NORTHWEST; CONIFER FORESTS; DYNAMICS; REGIMES AB Ecological forestry practices are designed to retain species and structural features important for maintaining ecosystem function but which may be deficient in conventionally managed stands. We used the spatially-explicit, individual tree model CANOPY to assess tradeoffs in enhanced ecological attributes vs. reductions in timber yield for a wide variety of treatments in uneven-aged, late-successional northern hardwood forests. Treatments included various combinations of (1) larger retained maximum tree diameters in the post-harvest stand, (2) permanently reserved legacy trees, (3) variable opening sizes, (4) coarse woody debris retention, (5) species harvest restrictions, and (6) occasional moderate-intensity harvests with larger openings ('irregular multi-cohort harvests'). Compared to conventional single-tree selection, reduction in simulated harvest yields varied widely from a 9% decline with 7 reserve trees/ha to a 55% reduction in treatments that retained coarse woody debris along with a maximum residual live-tree diameter of 80 cm. Despite the dominance by shade-tolerant species, simulated declines were similar in magnitude to those predicted or observed for relatively shade-intolerant conifers of the Pacific Northwest. Treatments that protected 'sensitive' species from harvest or raised the maximum residual diameter to 80 cm appeared to have the best balance between fostering ecological values of old-growth forests and moderating the impact on timber yield. These treatments produced stands meeting minimum structural criteria of old-growth forests while causing harvest declines of 27-30% compared to conventional single-tree selection. Coarse woody debris volumes were similar to those produced by the reserve-tree treatments, but the species-protection and 80 cm treatments had higher densities of large trees, and there was less reduction in yield for each large tree retained in the residual stand. Most other treatments maintained mature forest structure or stands that vacillated between mature and borderline old-growth conditions. (C) 2012 Elsevier B.V. All rights reserved. C1 [Hanson, Jacob J.; Lorimer, Craig G.; Halpin, Corey R.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53706 USA. [Palik, Brian J.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Hanson, JJ (reprint author), 3041 46th Ave NW, Olympia, WA 98502 USA. EM jacobjhanson@gmail.com; clorimer@wisc.edu; crhalpin@wisc.edu; bpalik@fs.fed.us FU McIntire-Stennis Cooperative Forestry Research Program [WIS04684]; USDA Forest Service [04-JV11231300-046]; Connor Hardwood Research Fund; College of Agricultural and Life Sciences, University of Wisconsin-Madison FX This research was funded by the McIntire-Stennis Cooperative Forestry Research Program, project WIS04684, USDA Forest Service Joint Venture agreement 04-JV11231300-046, the Connor Hardwood Research Fund, and the College of Agricultural and Life Sciences, University of Wisconsin-Madison. Helpful comments on earlier versions of the manuscript were provided by E.L. Kruger, B. Yandell, J. Buongiorno, D.J. Mladenoff, and two anonymous reviewers. NR 65 TC 12 Z9 12 U1 9 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2012 VL 270 BP 94 EP 107 DI 10.1016/j.foreco.2012.01.017 PG 14 WC Forestry SC Forestry GA 928MM UT WOS:000302986900012 ER PT J AU Domke, GM Woodall, CW Smith, JE Westfall, JA McRoberts, RE AF Domke, Grant M. Woodall, Christopher W. Smith, James E. Westfall, James A. McRoberts, Ronald E. TI Consequences of alternative tree-level biomass estimation procedures on U.S. forest carbon stock estimates SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest inventory; Carbon accounting; Tree volume; Climate change; Tree biomass ID UNITED-STATES; SINK; EMISSIONS; EQUATIONS AB Forest ecosystems are the largest terrestrial carbon sink on earth and their management has been recognized as a relatively cost-effective strategy for offsetting greenhouse gas emissions. Forest carbon stocks in the U.S. are estimated using data from the USDA Forest Service, Forest Inventory and Analysis (FIA) program. In an attempt to balance accuracy with consistency, the FIA program recently developed the component ratio method which utilizes regional volume models to replace the existing set of generalized allometric regression models used to estimate biomass and carbon stocks. This study describes the impact of the transition from the generalized allometric regression models to the component ratio method on the National Greenhouse Gas Inventory estimates by comparing estimates of carbon stocks from both approaches by common tree species and varying spatial scales (e.g., tree to national scale). Results for the 20 most abundant tree species in the 48 conterminous states of the U.S. suggest there is a significant difference in estimates of carbon stocks at the plot and national scales for the two estimation approaches. The component ratio method decreased estimates of national carbon stocks by an average of 16% for the species in the study. The observed reductions in carbon estimates can be attributed to incorporation of tree height as a predictor variable into species-specific volume models used to estimate tree biomass and carbon stocks. While the transition from the generalized allometric regression models to the component ratio method is procedural in nature, it may have important implications for national and global forest carbon sink estimates and the perception of the role forests play in mitigating the effects of atmospheric carbon dioxide. By combining regional accuracy with a nationally consistent approach, the component ratio method reflects a critical first step in aligning estimates of forest carbon stocks in the U.S.'s National Greenhouse Gas Inventory with estimates of tree volume in the FIA database. Published by Elsevier B.V. C1 [Domke, Grant M.; Woodall, Christopher W.; McRoberts, Ronald E.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. [Smith, James E.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Westfall, James A.] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. RP Domke, GM (reprint author), US Forest Serv, USDA, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM gmdomke@fs.fed.us; cwoodall@fs.fed.us; jsmith11@fs.fed.us; jameswestfall@fs.fed.us; rmcroberts@fs.fed.us OI Woodall, Christopher/0000-0001-8076-6214; Domke, Grant/0000-0003-0485-0355 NR 25 TC 25 Z9 26 U1 1 U2 27 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2012 VL 270 BP 108 EP 116 DI 10.1016/j.foreco.2012.01.022 PG 9 WC Forestry SC Forestry GA 928MM UT WOS:000302986900013 ER PT J AU Lyons-Tinsley, C Peterson, DL AF Lyons-Tinsley, Christina Peterson, David L. TI Surface fuel treatments in young, regenerating stands affect wildfire severity in a mixed conifer forest, eastside Cascade Range, Washington, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire severity; Surface fuel; Tripod Fire; Plantations; Site preparation; Washington ID FIRE; CLIMATE; CONSERVATION; MORTALITY; ECOLOGY; FUTURE AB Previous studies have debated the flammability of young regenerating stands, especially those in a matrix of mature forest, and no consensus has emerged as to whether young stands are inherently prone to high-severity wildfire. This topic has recently been addressed using spatial imagery, and weak inferences were made given the scale mismatch between the coarse resolution of spatial imagery and the fine resolution of mechanisms driving fire severity. We collected empirical stand and fire-severity data from 44 regenerating stands that are interspersed in mature, mid-elevation forests in the Okanogan-Wenatchee National Forest (OWNF) on the eastside of the Cascade Range in Washington, USA. These stands are mixed-species plantations that were established to promote regeneration of seral to late-seral tree species (Douglas-fir, subalpine fir, Engelmann spruce, western larch) in small patches within a larger lodgepole pine forest. In 2006, the 70,925 ha Tripod Fire burned through all the plantations and the surrounding lodgepole pine matrix. To understand what drives fire effects in plantations, especially those that exist in spatially heterogeneous forests, we compared fire severity in plantations with and without fuels-reducing site preparation (i.e., fuel treatments), using three metrics to quantify severity: mortality (%), exposed mineral soil (%), and char height (m). We built generalized linear models for each severity metric and tested for a difference in all severity metrics between treated and untreated units using Permutational Multivariate Analysis of Variance. Units without fuel treatments have more severe fire effects: mortality is 77% in untreated units and 37% in treated units (p = 0.0005). Other variables contribute to differences in fire severity, including species composition, canopy closure, and canopy base height. Canopy base height and canopy closure both exhibit a reverse relationship with mortality from what was expected: the higher the canopy closure and the lower the canopy base height, the lower the mortality. In other words, stands that have trees closer together with crowns near the ground are more likely to have lower mortality. Overall, the results suggest that young stands in some dry mixed conifer forests can be resilient to wildfire if surface fuel loading is low upon stand establishment. (C) 2011 Elsevier B.V. All rights reserved. C1 [Lyons-Tinsley, Christina] Univ Washington, Fire & Mt Ecol Lab, Sch Forest Resources, Seattle, WA 98195 USA. [Peterson, David L.] US Forest Serv, USDA, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Lyons-Tinsley, C (reprint author), Univ Washington, Fire & Mt Ecol Lab, Sch Forest Resources, Box 352100, Seattle, WA 98195 USA. EM ltinsley@u.washington.edu; peterson@fs.fed.us RI Restaino, Christina/G-9690-2013 FU University of Washington; US. Forest Service Pacific Northwest Research Station; National Science Foundation FX We thank Don McKenzie, Jon Bakker, Patti Loesche, Susan Prichard, Tom Spies, and two anonymous reviewers chosen by the journal for reviewing earlier drafts of the manuscript. Robert Norheim created Figs. 4 and 5. Field assistance was provided by the Fire and Environmental Research Applications Team, with special thanks to Susan Prichard, Bob Vihananek, Shawn Smith, Kyle Jacobson, Joe Restaino, and Karen Kopper. Funding was provided by the Joint Fire Science Program, University of Washington, US. Forest Service Pacific Northwest Research Station, and a National Science Foundation Graduate Fellowship to the lead author. NR 46 TC 9 Z9 9 U1 3 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2012 VL 270 BP 117 EP 125 DI 10.1016/j.foreco.2011.04.016 PG 9 WC Forestry SC Forestry GA 928MM UT WOS:000302986900014 ER PT J AU Janisch, JE Wondzell, SM Ehinger, WJ AF Janisch, Jack E. Wondzell, Steven M. Ehinger, William J. TI Headwater stream temperature: Interpreting response after logging, with and without riparian buffers, Washington, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Headwater streams; Stream temperature; Forests; Logging; Riparian buffers; Pacific Northwest ID PACIFIC-NORTHWEST; COAST RANGE; LAND-USE; OREGON; HARVEST AB We examined stream temperature response to forest harvest in small (<9 ha) forested headwater catchments in western Washington, USA over a seven year period (2002-2008). These streams have very low discharge in late summer <(X)over bar> approximate to 0.3 L s(-1)) and many become spatially intermittent. We used a before-after, control-impact (BACI) study design to contrast the effect of clearcut logging with two riparian buffer designs, a continuous buffer and a patch buffer. We focused on maximum daily temperature throughout July and August, expecting to see large temperature increases in the clearcut streams (n = 5), much smaller increases in the continuously buffered streams (n = 6), with the patch-buffered streams (n = 5) intermediate. Statistical analyses indicated that all treatments resulted in significant (alpha = 0.05) increases in stream temperature. In the first year after logging, daily maximum temperatures during July and August increased in clearcut catchments by an average of 1.5 degrees C (range 0.2 to 3.6 degrees C), in patch-buffered catchments by 0.6 degrees C (range 0.1 to 1.2 degrees C), and in continuously buffered catchments by 1.1 degrees C (range 0.0 to 2.8 degrees C). Temperature responses were highly variable within treatments and, contrary to our expectations, stream temperature increases were small and did not follow expected trends among the treatment types. We conducted further analyses in an attempt to identify variables controlling the magnitude of post-harvest treatment responses. These analyses showed that the amount of canopy cover retained in the riparian buffer was not a strong explanatory variable. Instead, spatially intermittent streams with short surface-flowing extent above the monitoring station and usually characterized by coarse-textured streambed sediment tended to be thermally unresponsive. In contrast, streams with longer surface-flowing extent above the monitoring station and streams with substantial stream-adjacent wetlands, both of which were usually characterized by fine-textured streambed sediment, were thermally responsive. Overall, the area of surface water exposed to the ambient environment seemed to best explain our aggregate results. Results from our study suggest that very small headwater streams may be fundamentally different than many larger streams because factors other than shade from the overstory tree canopy can have sufficient influence on stream energy budgets to strongly moderate stream temperatures even following complete removal of the overstory canopy. (C) 2011 Elsevier B.V. All rights reserved. C1 [Janisch, Jack E.; Ehinger, William J.] Washington Dept Ecol, Environm Assessment Program, Olympia, WA 98504 USA. [Wondzell, Steven M.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. RP Janisch, JE (reprint author), Washington Dept Ecol, Environm Assessment Program, Mailstop 47710, Olympia, WA 98504 USA. EM jackjanisch@ecy.wa.gov; swondzell@fs.fed.us; william.ehinger@ecy.wa.gov FU Washington Department of Natural Resources; USDA Forest Service Pacific Northwest Research Station; Washington Department of Ecology FX This research was funded by grants from the Washington Department of Natural Resources. Additional funding was provided by the USDA Forest Service Pacific Northwest Research Station and the Washington Department of Ecology. The temperature analysis was part of the Riparian Ecosystem Management Study, an integrated research project conducted by the Washington Department of Natural Resources, the USDA Forest Service's Pacific Northwest Research Station, and the Washington Department of Ecology. We thank Pat Cunningham from the Pacific Northwest Research Station for statistical assistance. Shannon Cleason, Christopher Clinton, Brian Engeness, Stephanie Estrella, Alex Foster, Tiffany Foster, Jeremy Graham, Nicholas Grant, Chad Hill, Kevin Kennedy, Jordan Martinez, Jeremiah McMahan, Charlotte Milling, Brenda Nipp, Christen Noble, Tanya Roberts, Matias Rudback, Crystal Vancho, Troy Warnick, and Elizabeth Werner assisted with data collection and management. Steve Barrett, Washington Department of Ecology, wrote code to summarize daily temperatures. Comments of two anonymous referees further improved earlier drafts. The comments of Kathryn L. Ronnenberg also improved the paper. NR 37 TC 22 Z9 22 U1 4 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2012 VL 270 BP 302 EP 313 DI 10.1016/j.foreco.2011.12.035 PG 12 WC Forestry SC Forestry GA 928MM UT WOS:000302986900034 ER PT J AU Neubauer, JD Lulai, EC Thompson, AL Suttle, JC Bolton, MD AF Neubauer, Jonathan D. Lulai, Edward C. Thompson, Asunta L. Suttle, Jeffrey C. Bolton, Melvin D. TI Wounding coordinately induces cell wall protein, cell cycle and pectin methyl esterase genes involved in tuber closing layer and wound periderm development SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Phellogen; Potato; Suberization; Wound-heal; Wound periderm ID HYDROXYPROLINE-RICH GLYCOPROTEIN; POTATO-TUBER; SOLANUM-TUBEROSUM; EXTENSIN GENE; MATURATION; EXPRESSION; RESISTANCE; STRESS; GROWTH; SUBERIN AB Little is known about the coordinate induction of genes that may be involved in agriculturally important wound-healing events. In this study, wound-healing events were determined together with wound-induced expression profiles of selected cell cycle, cell wall protein, and pectin methyl esterase genes using two diverse potato genotypes and two harvests (NDTX4271-5R and Russet Burbank tubers: 2008 and 2009 harvests). By 5 d after wounding, the closing layer and a nascent phellogen had formed. Phellogen cell divisions generated phellem layers until cessation of cell division at 28 d after wounding for both genotypes and harvests. Cell cycle genes encoding epidermal growth factor binding protein (StEBP), cyclin-dependent kinase B (StCDKB) and cyclin-dependent kinase regulatory subunit (StCKS1At) were induced by 1 d after wounding; these expressions coordinated with related phellogen formation and the induction and cessation of phellem cell formation. Genes encoding the structural cell wall proteins extensin (StExt1)and extensin-like (StExtlk)were dramatically up-regulated by 1-5 d after wounding, suggesting involvement with closing layer and later phellem cell layer formation. Wounding up-regulated pectin methyl esterase genes (StPME and StPrePME); StPME expression increased during closing layer and phellem cell formation, whereas maximum expression of StPrePME occurred at 5-14 d after wounding, implicating involvement in later modifications for closing layer and phellem cell formation. The coordinate induction and expression profile of StTLRP, a gene encoding a cell wall strengthening "tyrosine- and lysine-rich protein," suggested a role in the formation of the closing layer followed by phellem cell generation and maturation. Collectively, the genes monitored were wound-inducible and their expression profiles markedly coordinated with closing layer formation and the index for phellogen layer meristematic activity during wound periderm development: results were more influenced by harvest than genotype. Importantly, StTLRP was the only gene examined that may be involved in phellogen cell wall thickening after cessation of phellogen cell division. Published by Elsevier GmbH. C1 [Neubauer, Jonathan D.; Lulai, Edward C.; Suttle, Jeffrey C.; Bolton, Melvin D.] ARS, USDA, Sugarbeet & Potato Unit, NCSL, Fargo, ND 58102 USA. [Thompson, Asunta L.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58102 USA. RP Lulai, EC (reprint author), ARS, USDA, Sugarbeet & Potato Unit, NCSL, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM ed.lulai@ars.usda.gov NR 39 TC 13 Z9 15 U1 2 U2 32 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD APR 15 PY 2012 VL 169 IS 6 BP 586 EP 595 DI 10.1016/j.jplph.2011.12.010 PG 10 WC Plant Sciences SC Plant Sciences GA 927FP UT WOS:000302892800005 PM 22251796 ER PT J AU Leuning, R van Gorsel, E Massman, WJ Isaac, PR AF Leuning, Ray van Gorsel, Eva Massman, William J. Isaac, Peter R. TI Reflections on the surface energy imbalance problem SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Energy balance closure; Eddy covariance flux measurements; Micrometeorology; Advective flux divergence ID SOIL HEAT-FLUX; FREQUENCY-RESPONSE CORRECTIONS; EDDY-COVARIANCE MEASUREMENTS; BALANCE EXPERIMENT EBEX-2000; PATH CO2 ANALYZERS; WATER-VAPOR; SONIC ANEMOMETER; NET RADIOMETERS; CARBON-DIOXIDE; MICROMETEOROLOGICAL OBSERVATIONS AB The 'energy imbalance problem' in micrometeorology arises because at most flux measurement sites the sum of eddy fluxes of sensible and latent heat (H + lambda E) is less than the available energy (A). Either eddy fluxes are underestimated or A is overestimated. Reasons for the imbalance are: (1) a failure to satisfy the fundamental assumption of one-dimensional transport that is necessary for measurements on a single tower to represent spatially-averaged fluxes to/from the underlying surface, and (2) measurement errors in eddy fluxes, net radiation and changes in energy storage in soils, air and biomass below the measurement height. Radiometer errors are unlikely to overestimate A significantly, but phase lags caused by incorrect estimates of the energy storage terms can explain why H + lambda E systematically underestimates A at half-hourly time scales. Energy closure is observed at only 8% of flux sites in the La Thuile dataset (http://www.fluxdata.org/DataInfo/default.aspx) with half-hourly averages but this increases to 45% of sites using 24 h averages because energy entering the soil, air and biomass in the morning is returned in the afternoon and evening. Unrealistically large and positive horizontal gradients in temperature and humidity are needed for advective flux divergences to explain the energy imbalance at half-hourly time scales. Imbalances between H + lambda E and A still occur in daily averages but the small residual energy imbalances are explicable by horizontal and vertical advective flux divergences. Systematic underestimates of the vertical heat flux also occur if horizontal u'T' covariances contaminate the vertical w'T' signal due to incorrect coordinate rotations. Closure of the energy balance is possible at half-hourly time scales by careful attention to all sources of measurement and data processing errors in the eddy covariance system and by accurate measurement of net radiation and every energy storage term needed to calculate available energy. (C) 2011 Elsevier B.V. All rights reserved. C1 [Leuning, Ray; van Gorsel, Eva] CSIRO Marine & Atmospher Res, Canberra, ACT 2601, Australia. [Massman, William J.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. [Isaac, Peter R.] Monash Univ, Sch Geog & Environm Sci, Clayton, Vic 3800, Australia. RP Leuning, R (reprint author), CSIRO Marine & Atmospher Res, POB 3023, Canberra, ACT 2601, Australia. EM ray.leuning@csiro.au RI van Gorsel, Eva/A-1699-2012 OI van Gorsel, Eva/0000-0002-9939-7217 FU Australian Climate Change Program FX We gratefully acknowledge the excellent technical assistance of Steve Zegelin, Dale Hughes, for installing and maintaining the Otway, Tumbarumba and Virginia flux stations, and thank Darren Hocking for Sturt Plains and John Franks for the GLEES site. Ian Harman and Vanessa Haverd plus two anonymous referees are thanked for valuable comments on the manuscript. Our analysis of the La Thuile dataset is only possible through the dedication and generosity of a very large number of researchers across the globe who have made their data available to the general research community. It is hoped that this paper repays their generosity is some small measure. This work was supported by the Australian Climate Change Program (RL and E v G). NR 72 TC 107 Z9 110 U1 5 U2 95 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD APR 15 PY 2012 VL 156 BP 65 EP 74 DI 10.1016/j.agrformet.2011.12.002 PG 10 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 924DG UT WOS:000302670500006 ER PT J AU Tang, XG Wang, ZM Liu, DW Song, KS Jia, MM Dong, ZY Munger, JW Hollinger, DY Bolstad, PV Goldstein, AH Desai, AR Dragoni, D Liu, XP AF Tang, Xuguang Wang, Zongming Liu, Dianwei Song, Kaishan Jia, Mingming Dong, Zhangyu Munger, J. William Hollinger, David Y. Bolstad, Paul V. Goldstein, Allen H. Desai, Ankur R. Dragoni, Danilo Liu, Xiuping TI Estimating the net ecosystem exchange for the major forests in the northern United States by integrating MODIS and AmeriFlux data SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE NEE; Eddy covariance; MODIS; EVI; LST; LSWI ID GROSS PRIMARY PRODUCTION; VEGETATION WATER-CONTENT; CARBON EXCHANGE; SOIL RESPIRATION; TERRESTRIAL ECOSYSTEMS; ENVIRONMENTAL CONTROLS; TEMPERATE FORESTS; EUROPEAN FORESTS; CO2 EXCHANGE; CLIMATE DATA AB The eddy covariance technique provides long-term continuous monitoring of site-specific net ecosystem exchange of CO2 (NEE) across a large range of forest types. However, these NEE estimates only represent fluxes at the scale of the tower footprint and need to be scaled up to quantify NEE over regions or continents. In the present study, we expanded a method developed previously and generated a new NEE model exclusively based on the Moderate Resolution Imaging Spectroradiometer (MODIS) products, including enhanced vegetation index (EVI), land surface water index (LSWI), land surface temperature (LST) and Terra nighttime LST'. This method, in our previous research, provided substantially good predictions of NEE and well reflected the seasonal dynamics of the deciduous broadleaf forest at the Harvard forest site. Studying NEE of forests in the middle-latitude regions of the Northern Hemisphere is significant because it may help to understand the 'missing carbon sink' from terrestrial ecosystems. In this study we selected eight eddy flux sites to represent the major forest ecosystems in the northern United States. Compared with the model based on a single site, we also established the general models that apply to evergreen needleleaf forest (ENF) and deciduous broadleaf forest (DBF), respectively. The results showed that our simpler model based entirely on MODIS products promised well to estimate NEE by the eddy covariance technique. The modeled annual mean NEE from DBF deviated from the measured NEE by 44.4%, whereas the modeled NEE from ENF was extremely close to the measured NEE within 5.5%. In the end, we also validated both general models for ENF and DBF using independent flux sites. It demonstrated this method performed well for estimating NEE. (C) 2012 Elsevier B.V. All rights reserved. C1 [Tang, Xuguang; Wang, Zongming; Liu, Dianwei; Song, Kaishan; Jia, Mingming; Dong, Zhangyu] Chinese Acad Sci, NE Inst Geog & Agroecol, Changchun 130012, Peoples R China. [Tang, Xuguang] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [Munger, J. William] Harvard Univ, Dept Earth & Planetary Sci, Cambridge, MA 02138 USA. [Hollinger, David Y.] US Forest Serv, No Res Stn, Durham, NH USA. [Bolstad, Paul V.] Univ Minnesota, Dept Forest Resources, St Paul, MN USA. [Goldstein, Allen H.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Desai, Ankur R.] Univ Wisconsin, Dept Atmospher & Ocean Sci, Madison, WI USA. [Dragoni, Danilo] Indiana Univ, Dept Geog, Bloomington, IN 47405 USA. [Liu, Xiuping] Chinese Acad Sci, Key Lab Agr Water Resources, Hebei Key Lab Agr Water Saving, Ctr Agr Resources Res,Inst Genet & Dev Biol, Shijiazhuang 050021, Peoples R China. RP Liu, DW (reprint author), Chinese Acad Sci, NE Inst Geog & Agroecol, Changchun 130012, Peoples R China. EM dianweiliu@gmail.com RI Hollinger, David/G-7185-2012; Goldstein, Allen/A-6857-2011; Desai, Ankur/A-5899-2008; Munger, J/H-4502-2013 OI Goldstein, Allen/0000-0003-4014-4896; Desai, Ankur/0000-0002-5226-6041; Munger, J/0000-0002-1042-8452 FU Chinese Academy of Sciences [XDA05050101, KZCX2-YW-QN305]; Department of Energy Terrestrial Carbon Processes; NASA; DOE National Institute for Climate Change Research (NICCR); USDA FX This study was supported by the Strategic Frontier Program of the Chinese Academy of Sciences (Grant no. XDA05050101) and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant no. KZCX2-YW-QN305). We thank the principal investigators and contributors of the MODIS products, the Distributed Active Archive Center of the Oak Ridge National Laboratory, and the Earth Observing System Data for making these MODIS data available. The towers were supported by Department of Energy Terrestrial Carbon Processes, NASA, DOE National Institute for Climate Change Research (NICCR), and the USDA. We also thank those involved in the maintenance, operation and collection of all the sites of the AmeriFlux network in the present study. NR 57 TC 19 Z9 20 U1 2 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD APR 15 PY 2012 VL 156 BP 75 EP 84 DI 10.1016/j.agrformet.2012.01.003 PG 10 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA 924DG UT WOS:000302670500007 ER PT J AU Gobster, PH Xiang, WN AF Gobster, Paul H. Xiang, Wei-Ning TI Article types SO LANDSCAPE AND URBAN PLANNING LA English DT Editorial Material C1 [Xiang, Wei-Ning] E China Normal Univ, Shanghai Key Lab Urban Ecol & Sustainabil, Shanghai 200062, Peoples R China. [Gobster, Paul H.] US Forest Serv, USDA, No Res Stn, Evanston, IL 60201 USA. RP Xiang, WN (reprint author), E China Normal Univ, Shanghai Key Lab Urban Ecol & Sustainabil, Shanghai 200062, Peoples R China. EM pgobster@gmail.com; wnxiang@mail.ecnu.edu.cn RI Gobster, Paul/A-2826-2013 OI Gobster, Paul/0000-0002-8576-0310 NR 0 TC 1 Z9 1 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD APR 15 PY 2012 VL 105 IS 3 BP 185 EP 186 DI 10.1016/j.landurbplan.2012.02.001 PG 2 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 920UD UT WOS:000302432500001 ER PT J AU Yan, L DeMars, LC AF Yan, Lin DeMars, Lana C. TI Non-motorized voluntary running does not affect experimental and spontaneous metastasis in mice SO CANCER RESEARCH LA English DT Meeting Abstract C1 [Yan, Lin; DeMars, Lana C.] ARS, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER ASSOC CANCER RESEARCH PI PHILADELPHIA PA 615 CHESTNUT ST, 17TH FLOOR, PHILADELPHIA, PA 19106-4404 USA SN 0008-5472 EI 1538-7445 J9 CANCER RES JI Cancer Res. PD APR 15 PY 2012 VL 72 SU 8 MA 595 DI 10.1158/1538-7445.AM2012-595 PG 2 WC Oncology SC Oncology GA V43SQ UT WOS:000209701503288 ER PT J AU Karaaslan, MA Tshabalala, MA Buschle-Diller, G AF Karaaslan, Muzaffer Ahmet Tshabalala, Mandla A. Buschle-Diller, Gisela TI Semi-interpenetrating polymer network hydrogels based on aspen hemicellulose and chitosan: Effect of crosslinking sequence on hydrogel properties SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE hemicellulose; hydrogel; film; semi-interpenetrating; chitosan ID MECHANICAL-PROPERTIES; POLY(ACRYLIC ACID); THERMAL-ANALYSIS; MORPHOLOGY; CELLULOSE; PROGRESS; XYLANS; FTIR AB Semi-interpenetrating network hydrogel films were prepared using hemicellulose and chemically crosslinked chitosan. Hemicellulose was extracted from aspen by using a novel alkaline treatment and characterized by HPSEC, and consisted of a mixture of high and low molecular weight polymeric fractions. HPLC analysis of the acid hydrolysate of the hemicellulose showed that its major constituent sugar was xylose. X-ray analysis showed that the relative crystallinity of hydrogels increased with increasing hemicellulose content up to 31.3%. Strong intermolecular interactions between chitosan and hemicellulose were evidenced by FT-IR analysis. Quantitative analysis of free amino groups showed that hemicellulose could interrupt the chemical crosslinking of chitosan macromolecules. Mechanical testing and swelling experiments were used to define the effective network crosslink density and average molecular weight between crosslinks. Swelling ratios increased with increasing hemicellulose content and mainly consisted of H-bonded bound water. Results revealed that by altering the hydrogel preparation steps and hemicellulose content, crosslink density and swelling behavior of semi-IPN hydrogels could be controlled without deteriorating their mechanical properties. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012 C1 [Karaaslan, Muzaffer Ahmet; Buschle-Diller, Gisela] Auburn Univ, Dept Polymer & Fiber Engn, Auburn, AL 36849 USA. [Tshabalala, Mandla A.] USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Buschle-Diller, G (reprint author), Auburn Univ, Dept Polymer & Fiber Engn, Auburn, AL 36849 USA. EM buschgi@auburn.com FU Wood Education and Resource Center (WERC) FX The authors are grateful for the support of the Wood Education and Resource Center (WERC). NR 40 TC 5 Z9 6 U1 6 U2 87 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0021-8995 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD APR 15 PY 2012 VL 124 IS 2 BP 1168 EP 1177 DI 10.1002/app.35075 PG 10 WC Polymer Science SC Polymer Science GA 870MF UT WOS:000298672800033 ER PT J AU Wang, CL Ulloa, M Mullens, TR Yu, JZ Roberts, PA AF Wang, Congli Ulloa, Mauricio Mullens, Teresa R. Yu, John Z. Roberts, Philip A. TI QTL Analysis for Transgressive Resistance to Root-Knot Nematode in Interspecific Cotton (Gossypium spp.) Progeny Derived from Susceptible Parents SO PLOS ONE LA English DT Article ID HELIANTHUS-PARADOXUS ASTERACEAE; WHEAT TRITICUM-AESTIVUM; ADULT-PLANT RESISTANCE; WILT DISEASE COMPLEX; F-SP TRITICI; STRIPE RUST; UPLAND COTTON; HIRSUTUM L.; RENIFORM NEMATODE; GENE RKN1 AB The southern root-knot nematode (RKN, Meloidogyne incognita) is a major soil-inhabiting plant parasite that causes significant yield losses in cotton (Gossypium spp.). Progeny from crosses between cotton genotypes susceptible to RKN produced segregants in subsequent populations which were highly resistant to this parasite. A recombinant inbred line (RIL) population of 138 lines developed from a cross between Upland cotton TM-1 (G. hirsutum L.) and Pima 3-79 (G. barbadense L.), both susceptible to RKN, was used to identify quantitative trait loci (QTLs) determining responses to RKN in greenhouse infection assays with simple sequence repeat (SSR) markers. Compared to both parents, 53.6% and 52.1% of RILs showed less (P<0.05) root-galling index (GI) and had lower (P<0.05) nematode egg production (eggs per gram root, EGR). Highly resistant lines (transgressive segregants) were identified in this RIL population for GI and/or EGR in two greenhouse experiments. QTLs were identified using the single-marker analysis nonparametric mapping Kruskal-Wallis test. Four major QTLs located on chromosomes 3, 4, 11, and 17 were identified to account for 8.0 to 12.3% of the phenotypic variance (R-2) in root-galling. Two major QTLs accounting for 9.7% and 10.6% of EGR variance were identified on chromosomes 14 and 23 (P<0.005), respectively. In addition, 19 putative QTLs (P<0.05) accounted for 4.5-7.7% of phenotypic variance (R-2) in GI, and 15 QTLs accounted for 4.2-7.3% of phenotypic variance in EGR. In lines with alleles positive for resistance contributed by both parents in combinations of two to four QTLs, dramatic reductions of >50% in both GI and EGR were observed. The transgressive segregants with epistatic effects derived from susceptible parents indicate that high levels of nematode resistance in cotton may be attained by pyramiding positive alleles using a QTL mapping approach. C1 [Wang, Congli; Mullens, Teresa R.; Roberts, Philip A.] Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA. [Ulloa, Mauricio] USDA ARS, Western Integrated Cropping Syst Res Unit, Shafter, CA USA. [Yu, John Z.] USDA ARS, So Plains Agr Res Ctr, College Stn, TX USA. RP Wang, CL (reprint author), Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA. EM philip.roberts@ucr.edu FU Cotton Incorporated University of California Discovery Grant [08-321, 06-10632]; Cotton Incorporated FX Funding provided by Cotton Incorporated (grant # 08-321) University of California Discovery Grant (Grant#06-10632). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.; Cotton Incorporated through their research grants program designed to address cotton field production problems, funded a portion of the research in this study conducted in the Roberts lab, in a manner typical of other funding agency programs. No other form of support from this funder was provided. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials. NR 52 TC 16 Z9 18 U1 1 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 13 PY 2012 VL 7 IS 4 AR e34874 DI 10.1371/journal.pone.0034874 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UT UT WOS:000305341600088 PM 22514682 ER PT J AU Colegate, SM Gardner, DR Joy, RJ Betz, JM Panter, KE AF Colegate, Steven M. Gardner, Dale R. Joy, Robert J. Betz, Joseph M. Panter, Kip E. TI Dehydropyrrolizidine Alkaloids, Including Monoesters with an Unusual Esterifying Acid, from Cultivated Crotalaria juncea (Sunn Hemp cv.'Tropic Sun') SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Crotalaria juncea; 'Tropic Sun'; pyrrolizidine alkaloids; N-oxides; HPLC-ESI/MS; junceine; trichodesmine; isohemijunceine; hemijunceine ID TOXIC RANGE PLANTS; PYRROLIZIDINE ALKALOIDS; N-OXIDES; SENECIO; MS; SPECTROSCOPY; CHEMISTRY AB Cultivation of Crotalaria juncea L. (Sunn Hemp cv. 'Tropic Sun') is recommended as a green manure crop in a rotation cycle to improve soil condition, help control erosion, suppress weeds, and reduce soil nematodes. Because C. juncea belongs to a genus that is known for the production of toxic dehydropyrrolizidine alkaloids, extracts of the roots, stems, leaves, and seeds of 'Tropic Sun' were analyzed for their presence using HPLC-ESI/MS. Qualitative analysis identified previously unknown alkaloids as major components along with the expected macrocyclic dehydropyrrolizidine alkaloid diesters, junceine and trichodesmine. The dehydropyrrolizidine alkaloids occurred mainly as the N-oxides in the roots, stems, and, to a lesser extent, leaves, but mainly as the free bases in the seeds. Comprehensive spectrometric and spectroscopic analysis enabled elucidation of the unknown alkaloids as diastereoisomers of isohemijunceine, a monoester of retronecine with an unusual necic acid. The dehydropyrrolizidine alkaloid contents of the roots, stems, and leaves of immature plants were estimated to be 0.05, 0.12, and 0.01% w/w, respectively, whereas seeds were estimated to contain 0.15% w/w. C1 [Colegate, Steven M.; Gardner, Dale R.; Panter, Kip E.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Joy, Robert J.] USDA, Nat Resources Conservat Serv, Plant Mat Ctr, Hoolehua, HI 96729 USA. [Betz, Joseph M.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. RP Colegate, SM (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM steven.colegate@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements of the National Institutes of Health FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health. NR 30 TC 18 Z9 18 U1 0 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 11 PY 2012 VL 60 IS 14 BP 3541 EP 3550 DI 10.1021/jf205296s PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 922CS UT WOS:000302525000001 PM 22429238 ER PT J AU Rounds, L Havens, CM Feinstein, Y Friedman, M Ravishankar, S AF Rounds, Liliana Havens, Cody M. Feinstein, Yelena Friedman, Mendel Ravishankar, Sadhana TI Plant Extracts, Spices, and Essential Oils Inactivate Escherichia coli O157:H7 and Reduce Formation of Potentially Carcinogenic Heterocyclic Amines in Cooked Beef Patties SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Escherichia coli O157:H7; heterocyclic amines; plant compounds; ground beef patties ID VIRGIN OLIVE OIL; ANTIMICROBIAL ACTIVITY; GROUND-BEEF; SALMONELLA-ENTERICA; PHENOLIC-COMPOUNDS; AROMATIC-AMINES; FRIED BEEF; ANTIBACTERIAL ACTIVITIES; LISTERIA-MONOCYTOGENES; CHEMICAL-COMPOSITION AB Meats need to be heated to inactivate foodborne pathogens such as Escherichia coli O157:H7. High-temperature treatment used to prepare well-done meats increases the formation of carcinogenic heterocyclic amines (HCAs). We evaluated the ability of plant extracts, spices, and essential oils to simultaneously inactivate E. coli O157:H7 and suppress HCA formation in heated hamburger patties. Ground beef with added antimicrobials was inoculated with E. coli O157:H7 (10(7) CFU/g). Patties were cooked to reach 45 degrees C at the geometric center, flipped, and cooked for 5 min. Samples were then taken for microbiological and mass spectrometry analysis of HCAs. Some compounds were inhibitory only against E. coli or HCA formation, while some others inhibited both. Addition of 5% olive or apple skin extracts reduced E. coli O157:H7 populations to below the detection limit and by 1.6 log CFU/g, respectively. Similarly, 1% lemongrass oil reduced E. coli O157:H7 to below detection limits, while clove bud oil reduced the pathogen by 1.6 log CFU/g. The major heterocyclic amines 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) were concurrently reduced with the addition of olive extract by 79.5% and 84.3% and with apple extract by 76.1% and 82.1%, respectively. Similar results were observed with clove bud oil: MeIQx and PhIP were reduced by 35% and 52.1%, respectively. Addition of onion powder decreased formation of PhIP by 94.3%. These results suggest that edible natural plant compounds have the potential to prevent foodborne infections as well as carcinogenesis in humans consuming heat-processed meat products. C1 [Rounds, Liliana; Havens, Cody M.; Ravishankar, Sadhana] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA. [Feinstein, Yelena] Univ Arizona, Ctr Toxicol, Tucson, AZ 85721 USA. [Friedman, Mendel] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Ravishankar, S (reprint author), Univ Arizona, Dept Vet Sci & Microbiol, 1117 E Lowell St, Tucson, AZ 85721 USA. EM sadhravi@email.arizona.edu OI Friedman, Mendel/0000-0003-2582-7517 FU American Cancer Society through the Arizona Cancer Center; College of Agriculture and Life Sciences at the University of Arizona FX This research was supported by the American Cancer Society through the Arizona Cancer Center. We thank the College of Agriculture and Life Sciences at the University of Arizona for their financial support. We also thank Dr. Roberto Crea for a gift of Hidrox-12. NR 71 TC 24 Z9 26 U1 4 U2 60 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 11 PY 2012 VL 60 IS 14 BP 3792 EP 3799 DI 10.1021/jf204062p PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 922CS UT WOS:000302525000031 PM 22397498 ER PT J AU Zimkus, BM Lawson, L Loader, SP Hanken, J AF Zimkus, Breda M. Lawson, Lucinda Loader, Simon P. Hanken, James TI Terrestrialization, Miniaturization and Rates of Diversification in African Puddle Frogs (Anura: Phrynobatrachidae) SO PLOS ONE LA English DT Article ID ANCESTRAL CHARACTER STATES; BODY-SIZE; LIFE-HISTORY; DISCRETE CHARACTERS; SKELETAL MORPHOLOGY; TAXONOMIC DIVERSITY; MAXIMUM-LIKELIHOOD; REPRODUCTIVE MODE; SPECIES-RICHNESS; EVOLUTION AB Terrestrialization, the evolution of non-aquatic oviposition, and miniaturization, the evolution of tiny adult body size, are recurring trends in amphibian evolution, but the relationships among the traits that characterize these phenomena are not well understood. Furthermore, these traits have been identified as possible "key innovations'' that are predicted to increase rates of speciation in those lineages in which they evolve. We examine terrestrialization and miniaturization in sub-Saharan puddle frogs (Phrynobatrachidae) in a phylogenetic context to investigate the relationship between adaptation and diversification through time. We use relative dating techniques to ascertain if character trait shifts are associated with increased diversification rates, and we evaluate the likelihood that a single temporal event can explain the evolution of those traits. Results indicate alternate reproductive modes evolved independently in Phrynobatrachus at least seven times, including terrestrial deposition of eggs and terrestrial, non-feeding larvae. These shifts towards alternate reproductive modes are not linked to a common temporal event. Contrary to the "key innovations'' hypothesis, clades that exhibit alternate reproductive modes have lower diversification rates than those that deposit eggs aquatically. Adult habitat, pedal webbing and body size have no effect on diversification rates. Though these traits putatively identified as key innovations for Phrynobatrachus do not seem to be associated with increased speciation rates, they may still provide opportunities to extend into new niches, thus increasing overall diversity. C1 [Zimkus, Breda M.; Hanken, James] Harvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA. [Zimkus, Breda M.; Hanken, James] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Lawson, Lucinda] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. [Loader, Simon P.] Univ Basel, Inst Biogeog, Basel, Switzerland. RP Zimkus, BM (reprint author), Harvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA. EM bzimkus@oeb.harvard.edu FU Department of Organismic and Evolutionary Biology; Museum of Comparative Zoology, Harvard University; National Science Foundation [EF-0334939]; Museum of Comparative Zoology; Society for Integrative; Swiss National Science Foundation [31003A-133067] FX This study was supported by the Department of Organismic and Evolutionary Biology and the Museum of Comparative Zoology, Harvard University, and by National Science Foundation grant EF-0334939 (AmphibiaTree) to J. Hanken. Putnam expedition grants from the Museum of Comparative Zoology supported fieldwork by B. Zimkus in Ethiopia and Tanzania. Fieldwork in Tanzania was additionally supported by a Society for Integrative and Comparative Biology Fellowship for Graduate Student Travel. S. Loader is supported by a Swiss National Science Foundation grant (31003A-133067). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 80 TC 9 Z9 10 U1 0 U2 14 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 10 PY 2012 VL 7 IS 4 AR e35118 DI 10.1371/journal.pone.0035118 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959FE UT WOS:000305297500078 PM 22509392 ER PT J AU Hutton, CJ Brazier, RE Nicholas, AP Nearing, M AF Hutton, Christopher J. Brazier, Richard E. Nicholas, Andrew P. Nearing, Mark TI On the effects of improved cross-section representation in one-dimensional flow routing models applied to ephemeral rivers SO WATER RESOURCES RESEARCH LA English DT Article ID TRANSMISSION LOSSES; ARID CATCHMENT; TOPOGRAPHIC DATA; UNCERTAINTY; PREDICTION; INFILTRATION; ROUGHNESS; DYNAMICS; CHANNELS; EVENT AB Flash floods are an important component of the semiarid hydrological cycle, and provide the potential for groundwater recharge as well as posing a dangerous natural hazard. A number of catchment models have been applied to flash flood prediction; however, in general they perform poorly. This study has investigated whether the incorporation of light detection and ranging (lidar) derived data into the structure of a 1-D flow routing model can improve the prediction of flash floods in ephemeral channels. Two versions of this model, one based on an existing trapezoidal representation of cross-section morphology (K-Tr), and one that uses lidar data (K-Li) were applied to 5 discrete runoff events measured at two locations on the main channel of The Walnut Gulch Experimental Watershed, United States. In general, K-Li showed improved performance in comparison to K-Tr, both when each model was calibrated to individual events and during an evaluation phase when the models (and parameter sets) were applied across events. Sensitivity analysis identified that the K-Li model also had more consistency in behavioral parameter sets across runoff events. In contrast, parameter interaction within K-Tr resulted in poorly constrained behavioral parameter sets across the multidimensional parameter space. These results, revealed with a modeling focus on the structure of a particular element of a distributed catchment model, suggest that lidar derived cross-section morphology can lead to improved, and more robust flash flood prediction. C1 [Hutton, Christopher J.] Univ Exeter, Coll Engn Math & Phys Sci, Ctr Water Syst, Exeter EX4 4QF, Devon, England. [Hutton, Christopher J.; Brazier, Richard E.; Nicholas, Andrew P.] Univ Exeter, Coll Life & Environm Sci, Exeter EX4 4RJ, Devon, England. [Nearing, Mark] USDA, SW Watershed Res Ctr, Tucson, AZ 85719 USA. RP Hutton, CJ (reprint author), Univ Exeter, Coll Engn Math & Phys Sci, Ctr Water Syst, Harrison Bldg,N Pk Rd, Exeter EX4 4QF, Devon, England. EM c.j.hutton@ex.ac.uk FU University of Exeter; USDA-ARS Southwest Watershed Research Center FX The research was primarily funded by a University of Exeter Graduate Fellowship, awarded to the first author. Significant support was also provided by The USDA-ARS Southwest Watershed Research Center. NR 59 TC 3 Z9 3 U1 1 U2 19 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 J9 WATER RESOUR RES JI Water Resour. Res. PD APR 10 PY 2012 VL 48 AR W04509 DI 10.1029/2011WR011298 PG 11 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 926ZJ UT WOS:000302873300001 ER PT J AU Zelinka, SL Lambrecht, MJ Glass, SV Wiedenhoeft, AC Yelle, DJ AF Zelinka, Samuel L. Lambrecht, Michael J. Glass, Samuel V. Wiedenhoeft, Alex C. Yelle, Daniel J. TI Examination of water phase transitions in Loblolly pine and cell wall components by differential scanning calorimetry SO THERMOCHIMICA ACTA LA English DT Article DE Differential scanning calorimetry (DSC); Wood; Cellulose; Wood-water relations; Thermodynamics; Sorption ID FIBER SATURATION POINT; TIME-DOMAIN NMR; NORWAY SPRUCE; BOUND WATER; HYDROPHILIC POLYMERS; CELLULOSE FIBERS; WOOD; MOISTURE; DERIVATIVES; MODEL AB This paper examines phase transformations of water in wood and isolated wood cell wall components using differential scanning calorimetry with the purpose of better understanding "Type II water" or "freezable bound water" that has been reported for cellulose and other hydrophilic polymers. Solid loblolly pine (Pinus taeda) milled pine, and holocellulose, cellulose, and lignin isolated from the same parent board were tested. For each sample preparation, the freezing and melting of water was examined at 10-20 different moisture contents. Only one freezing peak was observed for most sample preparations; in these cases, the phase transformation corresponded to the "Type I" or "free water" peak. The freezing and melting temperatures of this Type I peak depended on moisture content, and appreciable undercooling (-30 degrees C from bulk water) was observed at the lowest moisture contents. The isolated ball-milled cellulose exhibited a Type II peak, yet the Wiley-milled cellulose, from which the ball-milled cellulose was made, did not exhibit a Type II peak. When present, the Type II peak is consistent with the homogeneous nucleation temperature of bulk water. The results suggest that the detection of Type II or freezable bound water in wood may depend more on sample preparation than the chemical nature of the cell wall component in question; a point not discussed by previous researchers describing Type II water in hydrophilic polymers. Published by Elsevier B.V. C1 [Zelinka, Samuel L.; Glass, Samuel V.; Wiedenhoeft, Alex C.; Yelle, Daniel J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Lambrecht, Michael J.] Univ Illinois, Dept Chem, Chicago, IL 60680 USA. RP Zelinka, SL (reprint author), US Forest Serv, Forest Prod Lab, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM szelinka@fs.fed.us FU U.S. Federal Highway Administration FX The authors acknowledge funding from the U.S. Federal Highway Administration under the Cooperative Research Program for Covered Bridges. This article was written and prepared by US Government employees on official time, and it is therefore in the public domain and not subject to copyright. This article was improved by critical comments from two anonymous reviewers. NR 41 TC 12 Z9 12 U1 0 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0040-6031 J9 THERMOCHIM ACTA JI Thermochim. Acta PD APR 10 PY 2012 VL 533 BP 39 EP 45 DI 10.1016/j.tca.2012.01.015 PG 7 WC Thermodynamics; Chemistry, Analytical; Chemistry, Physical SC Thermodynamics; Chemistry GA 923US UT WOS:000302646000006 ER PT J AU Marcot, BG AF Marcot, Bruce G. TI Metrics for evaluating performance and uncertainty of Bayesian network models SO ECOLOGICAL MODELLING LA English DT Article DE Bayesian network model; Uncertainty; Model performance; Model validation; Sensitivity analysis; Error rates; Probability analysis ID SPECIES DISTRIBUTION MODELS; NATURAL-RESOURCE MANAGEMENT; BELIEF NETWORKS; SELECTION; INEQUALITY; CHALLENGES; EVENNESS; BEHAVIOR; ECOLOGY AB This paper presents a selected set of existing and new metrics for gauging Bayesian network model performance and uncertainty. Selected existing and new metrics are discussed for conducting model sensitivity analysis (variance reduction, entropy reduction, case file simulation); evaluating scenarios (influence analysis); depicting model complexity (numbers of model variables, links, node states, conditional probabilities, and node cliques); assessing prediction performance (confusion tables, covariate and conditional probability-weighted confusion error rates, area under receiver operating characteristic curves, k-fold cross-validation, spherical payoff, Schwarz' Bayesian information criterion, true skill statistic, Cohen's kappa); and evaluating uncertainty of model posterior probability distributions (Bayesian credible interval, posterior probability certainty index, certainty envelope, Gini coefficient). Examples are presented of applying the metrics to 3 real-world models of wildlife population analysis and management. Using such metrics can vitally bolster model credibility, acceptance, and appropriate application, particularly when informing management decisions. Published by Elsevier B.V. C1 US Forest Serv, Pacific NW Res Stn, Portland, OR 97205 USA. RP Marcot, BG (reprint author), US Forest Serv, Pacific NW Res Stn, 620 SW Main St, Portland, OR 97205 USA. EM bmarcot@fs.fed.us NR 49 TC 56 Z9 57 U1 8 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 J9 ECOL MODEL JI Ecol. Model. PD APR 10 PY 2012 VL 230 BP 50 EP 62 DI 10.1016/j.ecolmodel.2012.01.013 PG 13 WC Ecology SC Environmental Sciences & Ecology GA 921ZS UT WOS:000302517200006 ER PT J AU Su, CL Khan, A Zhou, P Majumdar, D Ajzenberg, D Darde, ML Zhu, XQ Ajioka, JW Rosenthal, BM Dubey, JP Sibley, LD AF Su, Chunlei Khan, Asis Zhou, Peng Majumdar, Debashree Ajzenberg, Daniel Darde, Marie-Laure Zhu, Xing-Quan Ajioka, James W. Rosenthal, Benjamin M. Dubey, Jitender P. Sibley, L. David TI Globally diverse Toxoplasma gondii isolates comprise six major clades originating from a small number of distinct ancestral lineages SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE population structure; inheritance; toxoplasmosis ID POPULATION-STRUCTURE; CLINICAL FINDINGS; MOUSE-VIRULENCE; FRENCH-GUIANA; GENOTYPE; IDENTIFICATION; STRAINS; BRAZIL; EVOLUTIONARY; EXPANSION AB Marked phenotypic variation characterizes isolates of Toxoplasma gondii, a ubiquitous zoonotic parasite that serves as an important experimental model for studying apicomplexan parasites. Progress in identifying the heritable basis for clinically and epidemiologically significant differences requires a robust system for describing and interpreting evolutionary subdivisions in this prevalent pathogen. To develop such a system, we have examined more than 950 isolates collected from around the world and genotyped them using three independent sets of polymorphic DNA markers, sampling 30 loci distributed across all nuclear chromosomes as well as the plastid genome. Our studies reveal a biphasic pattern consisting of regions in the Northern Hemisphere where a few, highly clonal and abundant lineages predominate; elsewhere, and especially in portions of South America are characterized by a diverse assemblage of less common genotypes that show greater evidence of recombination. Clustering methods were used to organize the marked genetic diversity of 138 unique genotypes into 15 haplogroups that collectively define six major clades. Analysis of gene flow indicates that a small number of ancestral lineages gave rise to the existing diversity through a process of limited admixture. Identification of reference strains for these major groups should facilitate future studies on comparative genomics and identification of genes that control important biological phenotypes including pathogenesis and transmission. C1 [Rosenthal, Benjamin M.; Dubey, Jitender P.] ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Su, Chunlei; Majumdar, Debashree] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. [Khan, Asis; Sibley, L. David] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA. [Zhou, Peng] Chinese Acad Agr Sci, Shanghai Vet Res Inst, Minist Agr, Key Lab Anim Parasitol, Shanghai 200241, Peoples R China. [Ajzenberg, Daniel; Darde, Marie-Laure] Ctr Hosp Univ Dupuytren, Ctr Natl Reference Toxoplasmose, Toxoplasma Biol Resource Ctr, F-87042 Limoges, France. [Ajzenberg, Daniel; Darde, Marie-Laure] Univ Limoges, Fac Med, Lab Parasitol Mycol, INSERM,Unite Mixte Rech 1094, F-87025 Limoges, France. [Zhu, Xing-Quan] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China. [Ajioka, James W.] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England. RP Dubey, JP (reprint author), ARS, Anim Parasit Dis Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov; sibley@borcim.wus RI Su, Chunlei/M-1892-2013; OI Su, Chunlei/0000-0001-8392-7108; Rosenthal, Benjamin/0000-0002-0224-3773 FU National Institutes of Health [AI059176] FX We thank Jon Boyle, Michael Grigg, David Roos, and Jeroen Saeij for their advice and the Biological Resource Center Toxoplasma network for supplying some strains used here. This work was supported by Grant AI059176 from the National Institutes of Health (to L.D.S.). NR 44 TC 108 Z9 115 U1 1 U2 39 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 10 PY 2012 VL 109 IS 15 BP 5844 EP 5849 DI 10.1073/pnas.1203190109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 922FX UT WOS:000302533500059 PM 22431627 ER PT J AU Scully, ED Hoover, K Carlson, J Tien, M Geib, SM AF Scully, Erin D. Hoover, Kelli Carlson, John Tien, Ming Geib, Scott M. TI Analysis of Fusarium solani Isolated from the Longhorned Beetle, Anoplophora glabripennis SO PLOS ONE LA English DT Article ID TERMITE COPTOTERMES-FORMOSANUS; AMINO-ACID-SEQUENCE; PHANEROCHAETE-CHRYSOSPORIUM; LIGNIN DEGRADATION; LIGNOCELLULOSE DEGRADATION; EXTRACELLULAR PEROXIDASE; MICROBIAL-DEGRADATION; CERAMBYCID BEETLES; LIGNICOLOUS FUNGI; WOOD DECAY AB Wood is a highly intractable food source, yet many insects successfully colonize and thrive in this challenging niche. Overcoming the lignin barrier of wood is a key challenge in nutrient acquisition, but full depolymerization of intact lignin polymers has only been conclusively demonstrated in fungi and is not known to occur by enzymes produced by insects or bacteria. Previous research validated that lignocellulose and hemicellulose degradation occur within the gut of the wood boring insect, Anoplophora glabripennis (Asian longhorned beetle), and that a fungal species, Fusarium solani (ATCC MYA 4552), is consistently associated with the larval stage. While the nature of this relationship is unresolved, we sought to assess this fungal isolate's ability to degrade lignocellulose and cell wall polysaccharides and to extract nutrients from woody tissue. This gut-derived fungal isolate was inoculated onto a wood-based substrate and shotgun proteomics using Multidimensional Protein Identification Technology (MudPIT) was employed to identify 400 expressed proteins. Through this approach, we detected proteins responsible for plant cell wall polysaccharide degradation, including proteins belonging to 28 glycosyl hydrolase families and several cutinases, esterases, lipases, pectate lyases, and polysaccharide deacetylases. Proteinases with broad substrate specificities and ureases were observed, indicating that this isolate has the capability to digest plant cell wall proteins and recycle nitrogenous waste under periods of nutrient limitation. Additionally, several laccases, peroxidases, and enzymes involved in extracellular hydrogen peroxide production previously implicated in lignin depolymerization were detected. In vitro biochemical assays were conducted to corroborate MudPIT results and confirmed that cellulases, glycosyl hydrolases, xylanases, laccases, and Mn- independent peroxidases were active in culture; however, lignin- nd Mn- dependent peroxidase activities were not detected While little is known about the role of filamentous fungi and their associations with insects, these findings suggest that this isolate has the endogenous potential to degrade lignocellulose and extract nutrients from woody tissue. C1 [Scully, Erin D.] Penn State Univ, Huck Inst Life Sci, Intercoll Program Genet, University Pk, PA 16802 USA. [Hoover, Kelli] Penn State Univ, Ctr Chem Ecol, Dept Entomol, University Pk, PA 16802 USA. [Carlson, John] Penn State Univ, Sch Forest Resources, University Pk, PA 16802 USA. [Carlson, John] Chonnam Natl Univ, World Class Univ, Dept Bioenergy Sci & Technol, Kwangju, South Korea. [Tien, Ming] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [Geib, Scott M.] USDA ARS, Trop Crop & Commod Protect Res Unit, Pacific Basin Agr Res Ctr, Hilo, HI USA. RP Scully, ED (reprint author), Penn State Univ, Huck Inst Life Sci, Intercoll Program Genet, University Pk, PA 16802 USA. EM scott.geib@ars.usda.gov FU USDA-NRI-CRSEES [2008-35504-04464, 2009-35302-05286]; Alphawood Foundation, Chicago, Illinois; Pennsylvania State University College of Agricultural Sciences; World Class University from the Ministry of Education, Science and Technology of South Korea [R31-2009-000-20025-0] FX Funding for this project was provided by USDA-NRI-CRSEES grant 2008-35504-04464, USDA-NRI-CREES grant 2009-35302-05286, the Alphawood Foundation, Chicago, Illinois, a Seed Grant to Dr. Hoover from the Pennsylvania State University College of Agricultural Sciences. Dr. Carlson was partially supported by World Class University Project R31-2009-000-20025-0 grant from the Ministry of Education, Science and Technology of South Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 86 TC 16 Z9 16 U1 2 U2 52 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2012 VL 7 IS 4 AR e32990 DI 10.1371/journal.pone.0032990 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 955JO UT WOS:000305014500008 PM 22496740 ER PT J AU Linzmeier, AM Konstantinov, AS AF Linzmeier, Adelita M. Konstantinov, Alexander S. TI A new genus of leaf litter inhabiting Neotropical Monoplatina (Coleoptera: Chrysomelidae: Galerucinae: Alticini) SO ZOOTAXA LA English DT Article DE flea beetles; apterous; new genus; new species; leaf litter; Neotropical Region AB A new genus (Andersonaltica) containing five new species (A. denticulata, A. elongata, A. pecki-the type species of the genus, A. tuberosa, and A. valens) from Central America is described and illustrated. It is compared to Aedmon Clark, Apleuraltica Bechyne, Distigmoptera Blake, Hypolampsis Clark, and Pseudolampsis Horn and a key to identification of these genera is provided. C1 [Linzmeier, Adelita M.] Fundacao Univ Fed Grande Dourados, Fac Ciencias Biol & Ambientais, BR-79804980 Dourados, MS, Brazil. [Linzmeier, Adelita M.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst,Natl Museum Nat Hist, Washington, DC 20013 USA. RP Linzmeier, AM (reprint author), Fundacao Univ Fed Grande Dourados, Fac Ciencias Biol & Ambientais, Caixa Postal 322, BR-79804980 Dourados, MS, Brazil. EM alinzmeier@yahoo.com.br; alex.konstantinov@ars.usda.gov RI Linzmeier, Adelita/A-9239-2013 FU Smithsonian Institution Fellowship Office FX We are grateful to R. Anderson (Canadian Museum of Nature, Ottawa, Canada), J. T. Longino (The Evergreen State College, Olympia WA), and S. Peck (Ottawa, Canada) for their spectacular flea beetle collecting efforts over the years. We thank M. Buffington, M. L. Chamorro, and M. Gates (Systematic Entomology Laboratory, Washington, DC) for help with the digital cameras and image capture and editing. R. Kula and A. Norrbom (Systematic Entomology Laboratory, Washington, DC) and C. Staines (Department of Entomology, Smithsonian Institution, Washington DC, USA) thoroughly reviewed earlier versions of this manuscript and provided valuable suggestions. The Smithsonian Institution Fellowship Office granted a short term fellowship to the first author in March of 2011. USDA is an equal opportunity provider and employer. NR 5 TC 2 Z9 2 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD APR 6 PY 2012 IS 3260 BP 19 EP 32 PG 14 WC Zoology SC Zoology GA 939QL UT WOS:000303828100002 ER PT J AU Gorski, L AF Gorski, Lisa TI Selective Enrichment Media Bias the Types of Salmonella enterica Strains Isolated from Mixed Strain Cultures and Complex Enrichment Broths SO PLOS ONE LA English DT Article ID LISTERIA-MONOCYTOGENES; SEROTYPES; SAMPLES; IDENTIFICATION; LABORATORIES; TYPHIMURIUM; DIVERSITY; TRACKING; DIFFER AB For foodborne outbreak investigations it can be difficult to isolate the relevant strain from food and/or environmental sources. If the sample is contaminated by more than one strain of the pathogen the relevant strain might be missed. In this study mixed cultures of Salmonella enterica were grown in one set of standard enrichment media to see if culture bias patterns emerged. Nineteen strains representing four serogroups and ten serotypes were compared in four-strain mixtures in Salmonella-only and in cattle fecal culture enrichment backgrounds using Salmonella enrichment media. One or more strain(s) emerged as dominant in each mixture. No serotype was most fit, but strains of serogroups C2 and E were more likely to dominate enrichment culture mixtures than strains of serogroups B or C1. Different versions of Rappaport-Vassiliadis (RV) medium gave different patterns of strain dominance in both Salmonella-only and fecal enrichment culture backgrounds. The fittest strains belonged to serogroups C1, C2, and E, and included strains of S. Infantis, S. Thompson S. Newport, S. 6,8:d:-, and S. Give. Strains of serogroup B, which included serotypes often seen in outbreaks such as S. Typhimurium, S. Saintpaul, and S. Schwarzengrund were less likely to emerge as dominant strains in the mixtures when using standard RV as part of the enrichment. Using a more nutrient-rich version of RV as part of the protocol led to a different pattern of strains emerging, however some were still present in very low numbers in the resulting population. These results indicate that outbreak investigations of food and/or other environmental samples should include multiple enrichment protocols to ensure isolation of target strains of Salmonella. C1 ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. RP Gorski, L (reprint author), ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA USA. EM lisa.gorski@ars.usda.gov FU United States Department of Agriculture (USDA), Agricultural Research Service CRIS [5325-42000-046-00D]; National Research Initiative from the USDA National Institute of Food and Agriculture [2006-55212-16927, 2007-35212-18239] FX This work was supported by the United States Department of Agriculture (USDA), Agricultural Research Service CRIS project 5325-42000-046-00D and, in part, by National Research Initiative Competitive Grant numbers 2006-55212-16927 and 2007-35212-18239 from the USDA National Institute of Food and Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 28 TC 13 Z9 14 U1 2 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 4 PY 2012 VL 7 IS 4 AR e34722 DI 10.1371/journal.pone.0034722 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 953GO UT WOS:000304855200070 PM 22496847 ER PT J AU Kuykendall, LD Shao, JY Hartung, JS AF Kuykendall, L. David Shao, Jonathan Y. Hartung, John S. TI Conservation of Gene Order and Content in the Circular Chromosomes of 'Candidatus Liberibacter asiaticus' and Other Rhizobiales SO PLOS ONE LA English DT Article ID REAL-TIME PCR; GREENING DISEASE; SPECIES TREE; CITRUS; BACTERIA; PLANTS; IDENTIFICATION; COLONIZATION; EVOLUTIONARY; SEQUENCE AB 'Ca. Liberibacter asiaticus,' an insect-vectored, obligate intracellular bacterium associated with citrus-greening disease, also called "HLB," is a member of the Rhizobiales along with nitrogen-fixing microsymbionts Sinorhizobium meliloti and Bradyrhizobium japonicum, plant pathogen Agrobacterium tumefaciens and facultative intracellular mammalian pathogen Bartonella henselae. Comparative analyses of their circular chromosomes identified 514 orthologous genes shared among all five species. Shared among all five species are 50 identical blocks of microsyntenous orthologous genes (MOGs), containing a total of 283 genes. While retaining highly conserved genomic blocks of microsynteny, divergent evolution, horizontal gene transfer and niche specialization have disrupted macrosynteny among the five circular chromosomes compared. Highly conserved microsyntenous gene clusters help define the Rhizobiales, an order previously defined by 16S RNA gene similarity and herein represented by the three families: Bartonellaceae, Bradyrhizobiaceae and Rhizobiaceae. Genes without orthologs in the other four species help define individual species. The circular chromosomes of each of the five Rhizobiales species examined had genes lacking orthologs in the other four species. For example, 63 proteins are encoded by genes of 'Ca. Liberibacter asiaticus' not shared with other members of the Rhizobiales. Of these 63 proteins, 17 have predicted functions related to DNA replication or RNA transcription, and some of these may have roles related to low genomic GC content. An additional 17 proteins have predicted functions relevant to cellular processes, particularly modifications of the cell surface. Seventeen unshared proteins have specific metabolic functions including a pathway to synthesize cholesterol encoded by a seven-gene operon. The remaining 12 proteins encoded by 'Ca. Liberibacter asiaticus' genes not shared with other Rhizobiales are of bacteriophage origin. 'Ca. Liberibacter asiaticus' shares 11 genes with only Sinorhizobium meliloti and 12 genes are shared with only Bartonella henselae. C1 [Kuykendall, L. David; Shao, Jonathan Y.; Hartung, John S.] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD USA. RP Kuykendall, LD (reprint author), ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD USA. EM John.Hartung@ars.usda.gov FU United States Department of Agriculture FX This research was supported by intramural United States Department of Agriculture Funds funds. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 4 Z9 4 U1 1 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 4 PY 2012 VL 7 IS 4 AR e34673 DI 10.1371/journal.pone.0034673 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 953GO UT WOS:000304855200068 PM 22496839 ER PT J AU Dickey, LC Boateng, AA Goldberg, NM Mullen, CA Mihalcik, D AF Dickey, Leland C. Boateng, Akwasi A. Goldberg, Neil M. Mullen, Charles A. Mihalcik, David TI Condensation of Acetol and Acetic Acid Vapor and Nitrogen Using Sprayed Aqueous Liquid SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article ID OIL AB A cellulose-derived fraction of biomass pyrolysis vapor was simulated by evaporating acetol and acetic acid with heated nitrogen. After generating the vapor/nitrogen mixture, it was superheated in a tube oven and condensed by contact with an aqueous mist created by an ultrasonic spray nozzle. The rates of condensation and fractions of vapor condensed were determined for various fluid flow rates to estimate liquid flow rates necessary to obtain complete acetol vapor condensation. The effect of noncondensible gas on the condensation was determined by varying the ratio of nitrogen to acetol vapor. For standard conditions, no effect of nitrogen content on rate was seen for vapor contents greater than 10 mol %, which would include any practical fast pyrolysis vapor. Increasing the concentration of condensed vapor in the aqueous solution spray, up to 30 wt %, had little effect on the condensation rate. C1 [Dickey, Leland C.; Boateng, Akwasi A.; Goldberg, Neil M.; Mullen, Charles A.; Mihalcik, David] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Dickey, LC (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lelanddickey@verizon.net NR 11 TC 1 Z9 1 U1 2 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD APR 4 PY 2012 VL 51 IS 13 BP 5067 EP 5072 DI 10.1021/ie2017465 PG 6 WC Engineering, Chemical SC Engineering GA 926LX UT WOS:000302833600035 ER PT J AU Gardiner, SE Norelli, JL de Silva, N Fazio, G Peil, A Malnoy, M Horner, M Bowatte, D Carlisle, C Wiedow, C Wan, YZ Bassett, CL Baldo, AM Celton, JM Richter, K Aldwinckle, HS Bus, VGM AF Gardiner, Susan E. Norelli, John L. de Silva, Nihal Fazio, Gennaro Peil, Andreas Malnoy, Mickael Horner, Mary Bowatte, Deepa Carlisle, Charmaine Wiedow, Claudia Wan, Yizhen Bassett, Carole L. Baldo, Angela M. Celton, Jean-Marc Richter, Klaus Aldwinckle, Herb S. Bus, Vincent G. M. TI Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus 'Robusta 5' accessions SO BMC GENETICS LA English DT Article ID ERWINIA-AMYLOVORA STRAINS; RECEPTOR-LIKE PROTEINS; LEUCINE-RICH REPEAT; LINKAGE GROUP 3; DISEASE RESISTANCE; ARABIDOPSIS-THALIANA; SCAB RESISTANCE; MAJOR QTL; DIFFERENTIAL SUSCEPTIBILITY; STRUCTURAL DIVERSITY AB Background: Breeding of fire blight resistant scions and rootstocks is a goal of several international apple breeding programs, as options are limited for management of this destructive disease caused by the bacterial pathogen Erwinia amylovora. A broad, large-effect quantitative trait locus (QTL) for fire blight resistance has been reported on linkage group 3 of Malus 'Robusta 5'. In this study we identified markers derived from putative fire blight resistance genes associated with the QTL by integrating further genetic mapping studies with bioinformatics analysis of transcript profiling data and genome sequence databases. Results: When several defined E. amylovora strains were used to inoculate three progenies from international breeding programs, all with 'Robusta 5' as a common parent, two distinct QTLs were detected on linkage group 3, where only one had previously been mapped. In the New Zealand 'Malling 9' X 'Robusta 5' population inoculated with E. amylovora ICMP11176, the proximal QTL co-located with SNP markers derived from a leucine-rich repeat, receptor-like protein (MxdRLP1) and a closely linked class 3 peroxidase gene. While the QTL detected in the German 'Idared' X 'Robusta 5' population inoculated with E. amylovora strains Ea222_JKI or ICMP11176 was approximately 6 cM distal to this, directly below a SNP marker derived from a heat shock 90 family protein gene (HSP90). In the US 'Otawa3' X 'Robusta5' population inoculated with E. amylovora strains Ea273 or E2002a, the position of the LOD score peak on linkage group 3 was dependent upon the pathogen strains used for inoculation. One of the five MxdRLP1 alleles identified in fire blight resistant and susceptible cultivars was genetically associated with resistance and used to develop a high resolution melting PCR marker. A resistance QTL detected on linkage group 7 of the US population co-located with another HSP90 gene-family member and a WRKY transcription factor previously associated with fire blight resistance. However, this QTL was not observed in the New Zealand or German populations. Conclusions: The results suggest that the upper region of 'Robusta5' linkage group 3 contains multiple genes contributing to fire blight resistance and that their contributions to resistance can vary depending upon pathogen virulence and other factors. Mapping markers derived from putative fire blight resistance genes has proved a useful aid in defining these QTLs and developing markers for marker-assisted breeding of fire blight resistance. C1 [Gardiner, Susan E.; Bowatte, Deepa; Carlisle, Charmaine; Wiedow, Claudia] New Zealand Inst Plant & Food Res Ltd PFR Palmers, Manawatu Mail Ctr, Palmerston North 4442, New Zealand. [Norelli, John L.; Bassett, Carole L.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [de Silva, Nihal] PFR Mt Albert, Auckland Mail Ctr, Auckland 1142, New Zealand. [Fazio, Gennaro; Baldo, Angela M.] ARS, USDA, Plant Genet Resources Unit, Geneva, NY 14456 USA. [Peil, Andreas] JKI, Inst Breeding Res Hort & Fruit Crops, D-01326 Dresden, Germany. [Malnoy, Mickael] Ist Agr San Michele allAdige, Fdn E Mach, I-38010 San Michele All Adige, TN, Italy. [Horner, Mary; Bus, Vincent G. M.] PFR Hawkes Bay, Havelock N 4157, New Zealand. [Wan, Yizhen] NW A&F Univ, Coll Hort, Yangling 712100, Shaanxi, Peoples R China. [Celton, Jean-Marc] Univ Angers, INRA, UMR Genet & Hort GenHort, F-49071 Beaucouze, France. [Richter, Klaus] JKI, Inst Resistance Res & Stress, D-06484 Quedlinburg, Germany. [Aldwinckle, Herb S.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14456 USA. RP Gardiner, SE (reprint author), New Zealand Inst Plant & Food Res Ltd PFR Palmers, Manawatu Mail Ctr, Private Bag 11600, Palmerston North 4442, New Zealand. EM sue.gardiner@plantandfood.co.nz RI Gardiner, Susan/A-2477-2010; Wan, Yizhen/H-8493-2013; Bus, Vincent/B-1318-2011; malnoy, mickael/C-5007-2012; Wiedow, Claudia/O-9291-2016; OI Gardiner, Susan/0000-0002-4884-8743; Bus, Vincent/0000-0003-4071-4457; Wiedow, Claudia/0000-0003-3402-5460; Celton, Jean-Marc/0000-0002-1452-4812 FU National Research Initiative of the USDA Cooperative State Research, Education and Extension Service [2005-35300-15462]; New Zealand Foundation for Science, Technology and Research [C02X0406, C06X0810] FX We gratefully acknowledge Wilbur Hershberger (USDA-ARS, Kearneysville, WV) for valuable technical assistance in cloning the LRR receptor-like protein gene from Malus and RT-qPCR analysis of Prx genes, and Dr. Donna A. Lalli (USDA-APHIS, Riverdale, MD) for participation in putative gene selection. We also thank Dr. Tanja Dreo, National Institute of Biology, Ljubljana, Slovenia for researching the origins of Ea222_JKI and making us aware of the distinction between Ea strains Ea222 and Ea222_JKI. The project was partially supported by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service, grant number 2005-35300-15462 and by the New Zealand Foundation for Science, Technology and Research, Contracts no. C02X0406 and C06X0810. NR 107 TC 29 Z9 34 U1 3 U2 38 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD APR 3 PY 2012 VL 13 AR 25 DI 10.1186/1471-2156-13-25 PG 20 WC Genetics & Heredity SC Genetics & Heredity GA 006OQ UT WOS:000308828900001 PM 22471693 ER PT J AU Fernandez-Fueyo, E Ruiz-Duenas, FJ Ferreira, P Floudas, D Hibbett, DS Canessa, P Larrondo, LF James, TY Seelenfreund, D Lobos, S Polanco, R Tello, M Honda, Y Watanabe, T Watanabe, T San, RJ Kubicek, CP Schmoll, M Gaskell, J Hammel, KE St John, FJ Vanden Wymelenberg, A Sabat, G BonDurant, SS Syed, K Yadav, JS Doddapaneni, H Subramanian, V Lavin, JL Oguiza, JA Perez, G Pisabarro, AG Ramirez, L Santoyo, F Master, E Coutinho, PM Henrissat, B Lombard, V Magnuson, JK Kues, U Hori, C Igarashi, K Samejima, M Held, BW Barry, KW LaButti, KM Lapidus, A Lindquist, EA Lucas, SM Riley, R Salamov, AA Hoffmeister, D Schwenk, D Hadar, Y Yarden, O de Vries, RP Wiebenga, A Stenlid, J Eastwood, D Grigoriev, IV Berka, RM Blanchette, RA Kersten, P Martinez, AT Vicuna, R Cullen, D AF Fernandez-Fueyo, Elena Ruiz-Duenas, Francisco J. Ferreira, Patricia Floudas, Dimitrios Hibbett, David S. Canessa, Paulo Larrondo, Luis F. James, Tim Y. Seelenfreund, Daniela Lobos, Sergio Polanco, Ruben Tello, Mario Honda, Yoichi Watanabe, Takahito Watanabe, Takashi San, Ryu Jae Kubicek, Christian P. Schmoll, Monika Gaskell, Jill Hammel, Kenneth E. St John, Franz J. Vanden Wymelenberg, Amber Sabat, Grzegorz BonDurant, Sandra Splinter Syed, Khajamohiddin Yadav, Jagjit S. Doddapaneni, Harshavardhan Subramanian, Venkataramanan Lavin, Jose L. Oguiza, Jose A. Perez, Gumer Pisabarro, Antonio G. Ramirez, Lucia Santoyo, Francisco Master, Emma Coutinho, Pedro M. Henrissat, Bernard Lombard, Vincent Magnuson, Jon Karl Kuees, Ursula Hori, Chiaki Igarashi, Kiyohiko Samejima, Masahiro Held, Benjamin W. Barry, Kerrie W. LaButti, Kurt M. Lapidus, Alla Lindquist, Erika A. Lucas, Susan M. Riley, Robert Salamov, Asaf A. Hoffmeister, Dirk Schwenk, Daniel Hadar, Yitzhak Yarden, Oded de Vries, Ronald P. Wiebenga, Ad Stenlid, Jan Eastwood, Daniel Grigoriev, Igor V. Berka, Randy M. Blanchette, Robert A. Kersten, Phil Martinez, Angel T. Vicuna, Rafael Cullen, Dan TI Comparative genomics of Ceriporiopsis subvermispora and Phanerochaete chrysosporium provide insight into selective ligninolysis SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article ID VERSATILE PEROXIDASE; HEME PEROXIDASES; BASIDIOMYCETE; LACCASE; EXPRESSION; OXIDATION; SEQUENCE; FUNGUS; ENZYME; GENES AB Efficient lignin depolymerization is unique to the wood decay basidiomycetes, collectively referred to as white rot fungi. Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. To investigate the basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora and P. chrysosporium. Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium, respectively. In addition, the C. subvermispora genome contains at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains none. We also observed expansion of the number of C. subvermispora desaturase-encoding genes putatively involved in lipid metabolism. Microarray-based transcriptome analysis showed substantial up-regulation of several desaturase and MnP genes in wood-containing medium. MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. These results support the importance of MnP and a lignin degradation mechanism whereby cleavage of the dominant nonphenolic structures is mediated by lipid peroxidation products. Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and, following heterologous expression in Escherichia coli, the enzymes were shown to oxidize high redox potential substrates, but not Mn2+. Apart from oxidative lignin degradation, we also examined cellulolytic and hemicellulolytic systems in both fungi. In summary, the C. subvermispora genetic inventory and expression patterns exhibit increased oxidoreductase potential and diminished cellulolytic capability relative to P. chrysosporium. C1 [Gaskell, Jill; Hammel, Kenneth E.; St John, Franz J.; Kersten, Phil; Cullen, Dan] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. [Fernandez-Fueyo, Elena; Ruiz-Duenas, Francisco J.; Martinez, Angel T.] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain. [Ferreira, Patricia] Univ Zaragoza, Dept Biochem & Mol & Cellular Biol, Zaragoza 50018, Spain. [Ferreira, Patricia] Univ Zaragoza, Inst Biocomputat & Phys Complex Syst, Zaragoza 50018, Spain. [Floudas, Dimitrios; Hibbett, David S.] Clark Univ, Dept Biol, Worcester, MA 01610 USA. [Canessa, Paulo; Larrondo, Luis F.; Vicuna, Rafael] Pontificia Univ Catolica Chile, Dept Mol Genet & Microbiol, Fac Biol Sci, Santiago 7780344, Chile. [Canessa, Paulo; Larrondo, Luis F.; Vicuna, Rafael] Millennium Inst Fundamental & Appl Biol, Santiago 7780344, Chile. [James, Tim Y.] Univ Michigan, Dept Ecol & Evolut, Ann Arbor, MI 48109 USA. [Seelenfreund, Daniela; Lobos, Sergio] Univ Chile, Dept Biochem & Mol Biol, Fac Chem Sci & Pharmaceut, Santiago, Chile. [Polanco, Ruben] Univ Andres Bello, Fac Biol Sci, Dept Biol Sci, Santiago, Chile. [Tello, Mario] Univ Santiago Chile, Aquat Biotechnol Ctr, Dept Biol, Fac Chem & Biol, Santiago, Chile. [Honda, Yoichi; Watanabe, Takahito; Watanabe, Takashi] Kyoto Univ, Lab Biomass Convers, Res Inst Sustainable Humanosphere, Uji, Kyoto 6110011, Japan. [San, Ryu Jae] Gyeongnam Agr Res & Extens Serv, Dept Ecofriendliness Res, Gyeongnam 621802, South Korea. [Kubicek, Christian P.; Schmoll, Monika] Vienna Univ Technol, Res Area Biotechnol & Microbiol, Inst Chem Engn, A-1060 Vienna, Austria. [Kubicek, Christian P.] Vienna Univ Technol, Inst Chem Engn, Austrian Ctr Ind Biotechnol, A-1060 Vienna, Austria. [Vanden Wymelenberg, Amber] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Sabat, Grzegorz; BonDurant, Sandra Splinter] Univ Wisconsin, Ctr Biotechnol, Madison, WI 53706 USA. [Syed, Khajamohiddin; Yadav, Jagjit S.] Univ Cincinnati, Dept Environm Hlth, Cincinnati, OH 45267 USA. [Doddapaneni, Harshavardhan] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. [Subramanian, Venkataramanan] Colorado Sch Mines, Golden, CO 80401 USA. [Subramanian, Venkataramanan] Natl Renewable Energy Lab, Golden, CO 80401 USA. [Lavin, Jose L.; Oguiza, Jose A.; Perez, Gumer; Pisabarro, Antonio G.; Ramirez, Lucia; Santoyo, Francisco] Univ Publ Navarra, Genet & Microbiol Res Grp, Pamplona 31006, Spain. [Master, Emma] Univ Toronto, Dept Chem Engn, Toronto, ON M5S 3E5, Canada. [Coutinho, Pedro M.; Henrissat, Bernard; Lombard, Vincent] Aix Marseille Univ, CNRS, UMR 7257, F-13288 Marseille, France. [Magnuson, Jon Karl] Pacific NW Natl Lab, Richland, WA 99352 USA. [Kuees, Ursula] Univ Gottingen, Busgen Inst, D-37077 Gottingen, Germany. [Hori, Chiaki; Igarashi, Kiyohiko; Samejima, Masahiro] Univ Tokyo, Dept Biomat Sci, Tokyo 1138654, Japan. [Held, Benjamin W.; Blanchette, Robert A.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Barry, Kerrie W.; LaButti, Kurt M.; Lapidus, Alla; Lindquist, Erika A.; Lucas, Susan M.; Riley, Robert; Salamov, Asaf A.; Grigoriev, Igor V.] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. [Hoffmeister, Dirk; Schwenk, Daniel] Univ Jena, Dept Pharmaceut Biol, D-07745 Jena, Germany. [Hadar, Yitzhak; Yarden, Oded] Hebrew Univ Jerusalem, Dept Plant Pathol & Microbiol, IL-91120 Rehovot, Israel. [de Vries, Ronald P.; Wiebenga, Ad] Royal Netherlands Acad Arts & Sci, Fungal Biodivers Ctr, Cent Bur Schimmelcultures, NL-3584 CT Utrecht, Netherlands. [Stenlid, Jan] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, S-75007 Uppsala, Sweden. [Eastwood, Daniel] Swansea Univ, Dept Biosci, Swansea SA2 8PP, W Glam, Wales. [Berka, Randy M.] Novozymes, Davis, CA 95618 USA. RP Cullen, D (reprint author), US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM dcullen@wisc.edu RI Igarashi, Kiyohiko/E-6799-2016; Schmoll, Monika/I-6541-2016; Larrondo, Luis/J-1086-2016; Lavin, Jose Luis/J-7892-2013; St John, Franz/J-8970-2016; Hammel, Kenneth/G-1890-2011; Ruiz-Duenas, Francisco/L-9837-2015; Henrissat, Bernard/J-2475-2012; Yarden, Oded/J-5988-2012; Larrondo, Luis/A-2916-2013; Lapidus, Alla/I-4348-2013; Syed, Khajamohiddin/L-8774-2013; de Vries, Ronald/F-8125-2011; Pisabarro, Antonio/K-3622-2014; Ramirez, Lucia/K-3845-2014; Perez , Gumer/L-7970-2014; Master, Emma/O-3554-2014 OI Martinez, Angel T/0000-0002-1584-2863; Kues, Ursula/0000-0001-9180-4079; Igarashi, Kiyohiko/0000-0001-5152-7177; Schmoll, Monika/0000-0003-3918-0574; Larrondo, Luis/0000-0002-8832-7109; Lavin, Jose Luis/0000-0003-0914-3211; St John, Franz/0000-0003-3458-5628; Eastwood, Daniel/0000-0002-7015-0739; Tello, Mario/0000-0003-4573-6460; Hammel, Kenneth/0000-0002-2935-5847; Ruiz-Duenas, Francisco/0000-0002-9837-5665; Lapidus, Alla/0000-0003-0427-8731; Syed, Khajamohiddin/0000-0002-1497-3570; de Vries, Ronald/0000-0002-4363-1123; Pisabarro, Antonio/0000-0001-6987-5794; Ramirez, Lucia/0000-0002-0023-4240; FU US Department of Agriculture Cooperative State, Research, Education, and Extension Service [2007-35504-18257]; Office of Science of the US Department of Energy [DE-AC02-05CH11231]; European Project Peroxidases as Biocatalysts [KBBE-2010-4-265397]; Chilean National Fund for Scientific and Technological Development [1090513]; "Ramon y Cajal"; [BIO2008-01533]; [BIO2011-26694] FX We thank Sally Ralph (Forest Products Laboratory) for preparation of ball-milled wood. The major portions of this work were performed under US Department of Agriculture Cooperative State, Research, Education, and Extension Service Grant 2007-35504-18257 (to D.C. and R.A.B.). The US Department of Energy Joint Genome Institute is supported by the Office of Science of the US Department of Energy under Contract DE-AC02-05CH11231. This work was supported by Spanish Projects BIO2008-01533 and BIO2011-26694, European Project Peroxidases as Biocatalysts KBBE-2010-4-265397 (to F.J.R.-D. and A.T.M.), the Chilean National Fund for Scientific and Technological Development Grant 1090513 (to L.F.L.), and a "Ramon y Cajal" contract (to F.J.R.-D.). NR 33 TC 95 Z9 97 U1 4 U2 120 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 3 PY 2012 VL 109 IS 14 BP 5458 EP 5463 DI 10.1073/pnas.1119912109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 919BD UT WOS:000302294700070 PM 22434909 ER PT J AU Nicholson, SJ Hartson, SD Puterka, GJ AF Nicholson, Scott J. Hartson, Steven D. Puterka, Gary J. TI Proteomic analysis of secreted saliva from Russian Wheat Aphid (Diuraphis noxia Kurd.) biotypes that differ in virulence to wheat SO JOURNAL OF PROTEOMICS LA English DT Article DE Phytotoxic; Effectors; Plant defense; Elicitors; Pathogenic; Insect saliva ID ACYRTHOSIPHON-PISUM; HEMIPTERA APHIDIDAE; GENE-EXPRESSION; MYZUS-PERSICAE; ARABIDOPSIS-THALIANA; TREHALOSE METABOLISM; N-ACETYLGLUCOSAMINE; SHOTGUN PROTEOMICS; HEMOLYMPH-PROTEINS; STATISTICAL-MODEL AB Diuraphis noxia, Russian Wheat Aphid (RWA), biotypes are classified by their differential virulence to wheat varieties containing resistance genes. RWA salivary proteins, unlike those of most aphid species, cause foliar damage and physiological alterations in plants. A comparative proteomic analysis of secreted saliva from four differentially virulent RWA biotypes identified thirty-four individual proteins. The five major proteins were glucose dehydrogenase, lipophorin, chitinase, CiV16.8g1-like, and lava lamp. Fourteen proteins quantitatively varied among biotypes; trehalase, beta-N-acetylglucosaminidase (chitinase), two separate glucose dehydrogenases, calreticulin, aminopeptidase, acetylglucosaminyltransferase, hydroxymethylglutaryl-CoA lyase, acyltransferase, ficolin-3, lava lamp, retinaldehyde-binding protein, and two proteins of unknown function. Fifty-four percent of spectral counts were associated with glucose dehydrogenase, which is thought to detoxify plant defensive compounds. One-dimensional electrophoresis detected nine protein bands from 9 to 60 kDa that quantitatively differed. Two-dimensional electrophoresis identified six major gel zones with quantitative and qualitative variance in proteins. Our findings reveal that the salivary proteome of RWA, a phytotoxic aphid, differs considerably from those reported for nonphytotoxic aphids. The potential roles of proteins used in the general plant feeding processes of aphids and those that are potential phytotoxins related to aphid virulence are discussed. Published by Elsevier B.V. C1 [Nicholson, Scott J.] USDA ARS, PSRS, Plant Sci Res Lab, Stillwater, OK 74074 USA. [Hartson, Steven D.] Oklahoma State Univ, Dept Biochem & Mol Biol, Stillwater, OK 74078 USA. RP Nicholson, SJ (reprint author), USDA ARS, PSRS, Plant Sci Res Lab, 1301 N Western, Stillwater, OK 74074 USA. EM scott.nicholson@ars.usda.gov FU EPSCoR [0722494] FX Dr. Peter Hoyt, Yan Song, and Janet Rogers of the Oklahoma State University DNA/Protein Resource Facility provided invaluable assistance in collecting and analyzing LC-MS/MS data. Mass spectrometry analyses performed in the Oklahoma State University DNA/Protein Resource Facility are supported by the NSF MRI and EPSCoR programs (award #0722494). NR 117 TC 51 Z9 53 U1 9 U2 72 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 J9 J PROTEOMICS JI J. Proteomics PD APR 3 PY 2012 VL 75 IS 7 BP 2252 EP 2268 DI 10.1016/j.jprot.2012.01.031 PG 17 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA 930JV UT WOS:000303136600022 PM 22348819 ER PT J AU Khaokaew, S Landrot, G Chaney, RL Pandya, K Sparks, DL AF Khaokaew, Saengdao Landrot, Gautier Chaney, Rufus L. Pandya, Kaumudi Sparks, Donald L. TI Speciation and Release Kinetics of Zinc in Contaminated Paddy Soils SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID X-RAY-FLUORESCENCE; PRINCIPAL COMPONENT ANALYSIS; EXAFS SPECTROSCOPY; MICROMETER-SCALE; ZN SPECIATION; HEAVY-METALS; MONTMORILLONITE COMPOUNDS; NATURAL SPECIATION; CALCIUM-CARBONATE; ORGANIC-MATTER AB Zinc is an important nutrient for plants, but it can be toxic at high concentrations. The solubility and speciation of Zn is controlled by many factors, especially soil pH and Eh, which can vary in lowland rice culture. This study determined Zn speciation and release kinetics in Cd-Zn cocontaminated alkaline and acidified paddy soils, under various flooding periods and draining conditions, by employing synchrotron-based techniques and a stirred-flow kinetic method. Results showed almost no change in Zn speciation and release kinetics in the two soils, although the soils were subjected to different flooding periods and draining conditions. The mineral phases in which Zn is immobilized in the soil samples were constrained by linear least squares fitting (LLSF) analyses of bulk X-ray absorption fine structure (XAFS) spectra. Only two main phases were identified by LLSF, i.e., Zn-layered double hydroxides (Zn/Mg-hydrotalcite-like, and ZnAl-LDH) and Zn-phyllosilicates (Zn-kerolite). Under all soil pHs, flooding, and draining conditions, less than 22% of Zn was desorbed from the soil after a two-hour desorption experiment. The information on Zn chemistry obtained in this study will be useful in finding the best strategy to control Cd and Zr, bioavailability in the Cd-Zn cocontaminated paddy soils. C1 [Khaokaew, Saengdao; Landrot, Gautier; Sparks, Donald L.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. [Chaney, Rufus L.] ARS, USDA, Environm Chem Lab, Beltsville, MD 20705 USA. [Pandya, Kaumudi] Brookhaven Natl Lab, Natl Synchrotron Light Source, NRL SRC, Upton, NY 11026 USA. RP Khaokaew, S (reprint author), Kasetsart Univ, Dept Soil Sci, Fac Agr, 50 Ngam Wong Wan Rd, Ladyaow Chatuchak Bangko 10900, Thailand. EM agrsdk@yahoo.co.th FU Anandamahidol Foundation; Delaware Experimental Program to Stimulate Competitive Research (EPSCoR); National Science Foundation FX S.K. appreciates a graduate student fellowship from The Anandamahidol Foundation (King's scholarship) of King Bhumibol Adulyadej, the current King of Thailand. Financial support of the research was provided by the Delaware Experimental Program to Stimulate Competitive Research (EPSCoR) funded by the National Science Foundation. We also thank Dr. Matt Newville at beamline 13-ID, Advanced Photon Source (APS), Chicago, IL, for his assistance with mu-XRF data collection, and the four anonymous reviewers for their input that considerably improved the content of this manuscript. NR 60 TC 16 Z9 17 U1 11 U2 87 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD APR 3 PY 2012 VL 46 IS 7 BP 3957 EP 3963 DI 10.1021/es204007t PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 926SC UT WOS:000302850400049 PM 22423594 ER PT J AU O'Rourke, KI Schneider, DA Spraker, TR Dassanayake, RP Highland, MA Zhuang, DY Truscott, TC AF O'Rourke, Katherine I. Schneider, David A. Spraker, Terry R. Dassanayake, Rohana P. Highland, Margaret A. Zhuang, Dongyue Truscott, Thomas C. TI Transmissibility of caprine scrapie in ovine transgenic mice SO BMC VETERINARY RESEARCH LA English DT Article DE Prion; Mouse; Transgenic; Caprine ID CHRONIC WASTING DISEASE; PRION PROTEIN; PRPSC ACCUMULATION; SHEEP SCRAPIE; PET-BLOT; ENCEPHALOPATHY; GOATS; BRAIN; BSE; INFECTION AB Background: The United States control program for classical ovine scrapie is based in part on the finding that infection is typically spread through exposure to shed placentas from infected ewes. Transmission from goats to sheep is less well described. A suitable rodent model for examining the effect of caprine scrapie isolates in the ovine host will be useful in the ovine scrapie eradication effort. In this study, we describe the incubation time, brain lesion profile, glycoform pattern and PrPSc distribution patterns in a well characterized transgenic mouse line (Tg338) expressing the ovine VRQ prion allele, following inoculation with brain from scrapie infected goats. Results: First passage incubation times of caprine tissue in Tg338 ovinized mice varied widely but second passage intervals were shorter and consistent. Vacuolation profiles, glycoform patterns and paraffin-embedded tissue blots from terminally ill second passage mice derived from sheep or goat inocula were similar. Proteinase K digestion products of murine tissue were slightly smaller than the original ruminant inocula, a finding consistent with passage of several ovine strains in previous reports. Conclusions: These findings demonstrate that Tg338 mice propagate prions of caprine origin and provide a suitable baseline for examination of samples identified in the expanded US caprine scrapie surveillance program. C1 [O'Rourke, Katherine I.; Schneider, David A.; Zhuang, Dongyue; Truscott, Thomas C.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Spraker, Terry R.] Colorado State Univ, Dept Microbiol Immunol & Pathol, Coll Vet Med & Biomed Sci, Ft Collins, CO 80526 USA. [Dassanayake, Rohana P.; Highland, Margaret A.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP O'Rourke, KI (reprint author), Washington State Univ, USDA ARS, Pullman, WA 99164 USA. EM korourke@vetmed.wsu.edu RI Schneider, David/A-2833-2010 OI Schneider, David/0000-0001-9659-6731 FU USDA Agricultural Research Service [CRIS 5348-32000-026-00D] FX The USDA Agricultural Research Service provided financial support for this study under CRIS 5348-32000-026-00D. We thank L. Fuller, D. Chandler and K. Ross for care of the animals. L. Hamburg provided management of the Tg338 colony and performed the inoculations and necropsies. D. Lesiak provided PRNP genotyping. We thank S. Madsen-Bouterse and A. L. Brassfield for review of the manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 49 TC 10 Z9 10 U1 0 U2 2 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD APR 2 PY 2012 VL 8 AR 42 DI 10.1186/1746-6148-8-42 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA 039EV UT WOS:000311227800001 PM 22472560 ER PT J AU Rowland, LJ Alkharouf, N Darwish, O Ogden, EL Polashock, JJ Bassil, NV Main, D AF Rowland, Lisa J. Alkharouf, Nadim Darwish, Omar Ogden, Elizabeth L. Polashock, James J. Bassil, Nahla V. Main, Dorrie TI Generation and analysis of blueberry transcriptome sequences from leaves, developing fruit, and flower buds from cold acclimation through deacclimation SO BMC PLANT BIOLOGY LA English DT Article ID LOW-TEMPERATURE; EST; EXPRESSION; GENES; BIOSYNTHESIS; TOLERANCE; VACCINIUM; TAGS; ARABIDOPSIS; MARKERS AB Background: There has been increased consumption of blueberries in recent years fueled in part because of their many recognized health benefits. Blueberry fruit is very high in anthocyanins, which have been linked to improved night vision, prevention of macular degeneration, anti-cancer activity, and reduced risk of heart disease. Very few genomic resources have been available for blueberry, however. Further development of genomic resources like expressed sequence tags (ESTs), molecular markers, and genetic linkage maps could lead to more rapid genetic improvement. Marker-assisted selection could be used to combine traits for climatic adaptation with fruit and nutritional quality traits. Results: Efforts to sequence the transcriptome of the commercial highbush blueberry (Vaccinium corymbosum) cultivar Bluecrop and use the sequences to identify genes associated with cold acclimation and fruit development and develop SSR markers for mapping studies are presented here. Transcriptome sequences were generated from blueberry fruit at different stages of development, flower buds at different stages of cold acclimation, and leaves by next-generation Roche 454 sequencing. Over 600,000 reads were assembled into approximately 15,000 contigs and 124,000 singletons. The assembled sequences were annotated and functionally mapped to Gene Ontology (GO) terms. Frequency of the most abundant sequences in each of the libraries was compared across all libraries to identify genes that are potentially differentially expressed during cold acclimation and fruit development. Real-time PCR was performed to confirm their differential expression patterns. Overall, 14 out of 17 of the genes examined had differential expression patterns similar to what was predicted from their reads alone. The assembled sequences were also mined for SSRs. From these sequences, 15,886 blueberry EST-SSR loci were identified. Primers were designed from 7,705 of the SSR-containing sequences with adequate flanking sequence. One hundred primer pairs were tested for amplification and polymorphism among parents of two blueberry populations currently being used for genetic linkage map construction. The tetraploid mapping population was based on a cross between the highbush cultivars Draper and Jewel (V. darrowii is also in the background of 'Jewel'). The diploid mapping population was based on a cross between an F-1 hybrid of V. darrowii and diploid V. corymbosum and another diploid V. corymbosum. The overall amplification rate of the SSR primers was 68% and the polymorphism rate was 43%. Conclusions: These results indicate that this large collection of 454 ESTs will be a valuable resource for identifying genes that are potentially differentially expressed and play important roles in flower bud development, cold acclimation, chilling unit accumulation, and fruit development in blueberry and related species. In addition, the ESTs have already proved useful for the development of SSR and EST-PCR markers, and are currently being used for construction of genetic linkage maps in blueberry. C1 [Rowland, Lisa J.; Ogden, Elizabeth L.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, BARC W, Beltsville, MD 20705 USA. [Alkharouf, Nadim; Darwish, Omar] Towson Univ, Dept Comp & Informat Sci, Towson, MD 21252 USA. [Polashock, James J.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, Blueberry & Cranberry Res Ctr, Chatsworth, NJ 08019 USA. [Bassil, Nahla V.] USDA ARS, Corvallis, OR 97333 USA. [Main, Dorrie] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. RP Rowland, LJ (reprint author), USDA ARS, Genet Improvement Fruits & Vegetables Lab, BARC W, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Jeannine.Rowland@ars.usda.gov FU USDA/CSREES Specialty Crop Research Initiative program [2008-51180-04861] FX We would like to acknowledge Jeremy Jones for his technical support in evaluating SSR amplification and polymorphism and Brittany McCullough for her bioinformatic support. This work was supported by grant 2008-51180-04861 from the USDA/CSREES Specialty Crop Research Initiative program. NR 49 TC 37 Z9 45 U1 6 U2 69 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD APR 2 PY 2012 VL 12 AR 46 DI 10.1186/1471-2229-12-46 PG 18 WC Plant Sciences SC Plant Sciences GA 960XR UT WOS:000305427000001 PM 22471859 ER PT J AU Serengil, Y Swank, WT Vose, JM AF Serengil, Yusuf Swank, Wayne T. Vose, James M. TI Alterations on flow variability due to converting hardwood forests to pine SO IFOREST-BIOGEOSCIENCES AND FORESTRY LA English DT Article DE Flow variability; Conversion to pine; Ecological flows; High-low flow frequency ID GROUNDWATER-DOMINATED STREAM; MACROINVERTEBRATE COMMUNITY; CLIMATE-CHANGE; ECOSYSTEMS; MANAGEMENT; INDEXES; WATER; RESPONSES AB Flow variability is a potential indicator of land use impacts on aquatic ecosystems and a dominating factor for lotic habitats. Vegetation management effects on the stream habitat conditions must be better understood to propose forest management activities that are compatible with general ecosystem management objectives (integrity, diversity, sustainability, etc.). In our study, we used long term flow data (1936-2004) from four gauged experimental watersheds (W1, W2, W17, W18) of Coweeta Hydrologic Laboratory in US to assess the impacts of pine conversion on flow characteristics by using paired watershed experimentation. In W1, all trees and shrubs were cut and burned in 1956-57 and white pine (Pinus strobus) was planted in 1957. In W17, white pine was planted in 1956. W2 and W18 have been kept untreated as reference watersheds for W1 and W17, respectively. After analyzing long-term daily flow series with flow duration curves and frequency analyzes, we found that the timing and magnitude of 7Q flows were changed significantly due to conversion but flow variability was not affected. Overall findings revealed that pine conversion has significantly influenced some flow characteristics but stream habitat conditions were not affected potentially. C1 [Serengil, Yusuf] Istanbul Univ, Dept Watershed Management, Istanbul, Turkey. [Swank, Wayne T.; Vose, James M.] US Forest Serv, Coweeta Hydrol Lab, So Res Stn, USDA, Otto, NC 28763 USA. RP Serengil, Y (reprint author), Istanbul Univ, Dept Watershed Management, Istanbul, Turkey. EM serengil@istanbul.edu.tr NR 27 TC 0 Z9 0 U1 3 U2 18 PU SISEF-SOC ITALIANA SELVICOLTURA ECOL FORESTALE PI POTENZA PA DEPT PROD VEGETALE, VIA ATENEO LUCANO 10, POTENZA, 85100, ITALY SN 1971-7458 J9 IFOREST JI iForest PD APR 2 PY 2012 VL 5 BP 44 EP 49 DI 10.3832/ifor0609-009 PG 6 WC Forestry SC Forestry GA 935UR UT WOS:000303547600002 ER PT J AU Xu, YZ Wang, CP Stark, NM Cai, ZY Chu, FX AF Xu, Yuzhi Wang, Chunpeng Stark, Nicole M. Cai, Zhiyong Chu, Fuxiang TI Miscibility and thermal behavior of poly (epsilon-caprolactone)/long-chain ester of cellulose blends SO CARBOHYDRATE POLYMERS LA English DT Article DE Cellulose laurate; Poly (epsilon-caprolactone); Blends; Thermal behaviors; Miscibility ID POLY(EPSILON-CAPROLACTONE) BLENDS; PHYSICAL-PROPERTIES; ACETATE; BIODEGRADATION; MICROSCOPY; FILMS AB The long-chain cellulose ester (LCCE) cellulose laurate, poly(epsilon-caprolactone)(PCL) and their blends were characterized by tensile strength, Fourier transform infrared spectroscopy (FTIR), dynamic mechanical thermal analysis, thermogravimetric analysis (TGA), and transmission electron microscopy (TEM). The compatibility of the blends was investigated and the TEM results showed that PCL tended to disperse as discrete particles in LCCE. FTIR revealed that there were several specific interactions between the chains of PCL and LCCE: the interaction between C-H and O=C-,-C=O(sic)O=C-or-C=O(sic)O-C dipole-dipole interactions. The addition of PCL into LCCE increased slightly the tensile stress at break and the elongation up to 90/10 LCCE/PCL (w/w), which suggested partial compatibility between the polymers. In agreement with this, TGA showed that the compatibility of PCL and LCCE enhanced the thermal stability of LCCE in the blends. Together, these results indicated the existence of intermolecular interactions between the two polymers. (c) 2011 Elsevier Ltd. All rights reserved. C1 [Chu, Fuxiang] Chinese Acad Forestry, Inst New Technol, Beijing 100091, Peoples R China. [Xu, Yuzhi; Wang, Chunpeng] CAF, Inst Chem Ind Forest Prod, Nanjing 210042, Jiangsu, Peoples R China. [Stark, Nicole M.; Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Chu, FX (reprint author), Chinese Acad Forestry, Inst New Technol, Beijing 100091, Peoples R China. EM chufuxiang@forestry.ac.cn FU Technology Division of the State Forestry Bureau [201104004]; Chinese Academy of Forestry [CAFINT2009C03] FX The authors gratefully acknowledge the financial support from Technology Division of the State Forestry Bureau (201104004) and Chinese Academy of Forestry (CAFINT2009C03). NR 31 TC 8 Z9 8 U1 0 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD APR 2 PY 2012 VL 88 IS 2 BP 422 EP 427 DI 10.1016/j.carbpol.2011.11.079 PG 6 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 910JN UT WOS:000301633100003 ER PT J AU Han, WJ Zhao, CS Elder, T Chen, KF Yang, RD Kim, D Pu, YQ Hsieh, J Ragauskas, AJ AF Han, Wenjia Zhao, Chuanshan Elder, Thomas Chen, Kefu Yang, Rendang Kim, Dongho Pu, Yunqiao Hsieh, Jeffery Ragauskas, Arthur J. TI Study on the modification of bleached eucalyptus kraft pulp using birch xylan SO CARBOHYDRATE POLYMERS LA English DT Article DE Birch xylan; Eucalyptus pulp; Deposition; Mechanical properties; Morphology ID GLUCURONOXYLAN ADSORPTION; SOFTWOOD PULP; STRENGTH LOSS; CELLULOSE; FIBERS; PAPER; HEMICELLULOSES; HORNIFICATION; OPTIMIZATION AB In this study, birch xylan was deposited onto elementally chlorine free (ECF) bleached eucalyptus kraft pulp, and the corresponding changes in physical properties were determined. An aqueous 5% birch xylan solution at pH 9 was added to 5 wt% slurry of bleached kraft eucalyptus fibers, with stirring at 70 degrees C for 15 min after which the pH was adjusted to 5-6. The xylan enriched fibers were isolated by filtration and used for physical testing. A 1.15 wt% adsorption of birch xylan on the kraft fibers at 8% xylan addition increased the tensile index, strain and tensile energy absorption values by similar to 10%, while the burst index increased by 20.15%. The tear index increased by 2.55% with the adsorption of 0.87 wt% birch xylan on the eucalyptus kraft pulp at 3% xylan addition. The pulp beatability was also improved by adding birch xylan. The surface morphology of the unmodified and modified pulp samples were analyzed using atomic force microscopy (AFM) in the tapping mode. The analysis revealed the differences in the fine structure of fibers which showed micrometer-sized xylan structures spreading over the fiber surfaces. (c) 2012 Elsevier Ltd. All rights reserved. C1 [Han, Wenjia; Kim, Dongho; Pu, Yunqiao; Hsieh, Jeffery; Ragauskas, Arthur J.] Georgia Inst Technol, Inst Paper Sci & Technol, Atlanta, GA 30332 USA. [Han, Wenjia; Zhao, Chuanshan] Shandong Inst Light Ind, Minist Educ, Key Lab Paper Sci & Technol, Jinan 250353, Peoples R China. [Han, Wenjia; Chen, Kefu; Yang, Rendang] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangdong Publ Lab Paper Technol & Equipment, Guangzhou 510640, Guangdong, Peoples R China. [Elder, Thomas] US Forest Serv, So Res Stn, Pineville, LA 71360 USA. RP Ragauskas, AJ (reprint author), Georgia Inst Technol, Inst Paper Sci & Technol, 500 10th St NW, Atlanta, GA 30332 USA. EM arthur.sagauskas@ipst.gatech.edu OI Pu, Yunqiao/0000-0003-2554-1447 NR 32 TC 10 Z9 10 U1 1 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0144-8617 J9 CARBOHYD POLYM JI Carbohydr. Polym. PD APR 2 PY 2012 VL 88 IS 2 BP 719 EP 725 DI 10.1016/j.carbp01.2012.01.025 PG 7 WC Chemistry, Applied; Chemistry, Organic; Polymer Science SC Chemistry; Polymer Science GA 910JN UT WOS:000301633100043 ER PT J AU Adair, LS Thompson, AL Kayira, D Chasela, C Kacheche, Z Kamwendo, D Bentley, M Jamieson, D Allen, LH Shahab-Ferdows, S AF Adair, Linda S. Thompson, A. L. Kayira, D. Chasela, C. Kacheche, Z. Kamwendo, D. Bentley, M. Jamieson, D. Allen, L. H. Shahab-Ferdows, S. TI Maternal and infant predictors of CRP in exclusively breastfed infants born to HIV-infected Malawian mothers SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Adair, Linda S.; Thompson, A. L.; Bentley, M.] UNC, Chapel Hill, NC USA. [Kayira, D.; Chasela, C.; Kacheche, Z.; Kamwendo, D.] UNC Project, Lilongwe, Malawi. [Jamieson, D.] CDC, Atlanta, GA 30333 USA. [Allen, L. H.; Shahab-Ferdows, S.] ARS, USDA, Davis, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304788 ER PT J AU Ahuja, JKC Bhagwat, S Haytowitz, D Holden, J Bailey, R Dwyer, J Milner, J Moshfegh, A AF Ahuja, Jaspreet K. C. Bhagwat, Seema Haytowitz, David Holden, Joanne Bailey, Reagan Dwyer, Johanna Milner, John Moshfegh, Alanna TI Development of a flavonoid database for assessing population exposures and its application SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Ahuja, Jaspreet K. C.; Bhagwat, Seema; Haytowitz, David; Holden, Joanne; Moshfegh, Alanna] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Bailey, Reagan; Dwyer, Johanna] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Milner, John] NCI, NIH, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304421 ER PT J AU Ainslie-Waldman, C Young, LR Kurzer, MS Raatz, SK Csallany, AS AF Ainslie-Waldman, Cheryl Young, Lindsay R. Kurzer, Mindy S. Raatz, Susan K. Csallany, A. Saari TI The effect of dietary fat and omega-3 fatty acids on whole body lipid oxidation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Ainslie-Waldman, Cheryl; Young, Lindsay R.; Kurzer, Mindy S.; Csallany, A. Saari] Univ Minnesota, St Paul, MN 55108 USA. [Raatz, Susan K.] USDA, Human Nutr Res Ctr, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300490 ER PT J AU Anderson, CM Wright, ML Ralph, JL Uthus, EO Ohm, JE AF Anderson, Cindy M. Wright, Michelle L. Ralph, Jody L. Uthus, Eric O. Ohm, Joyce E. TI Epigenomic markers for heritable risk of preeclampsia SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Ohm, Joyce E.] Univ N Dakota, Dept Biochem & Mol Biol, Grand Forks, ND 58201 USA. [Uthus, Eric O.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RI Anderson, Cindy/I-3900-2013 NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305107 ER PT J AU Andrews, KW Roseland, JM Holden, JM Middleton, AM Solomon, AM Douglass, L Dwyer, JT Bailey, R Saldanha, LG Daniel, MG AF Andrews, K. W. Roseland, J. M. Holden, J. M. Middleton, A. M. Solomon, A. M. Douglass, L. Dwyer, J. T. Bailey, R. Saldanha, L. G. Daniel, M. G. TI Release 2 of the US Dietary Supplement Ingredient Database (DSID): research protocols and ingredient estimates for children's and adult multivitamins SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Andrews, K. W.; Roseland, J. M.; Holden, J. M.; Middleton, A. M.; Solomon, A. M.; Daniel, M. G.] USDA, Nutrient Data Lab, Beltsville, MD 20705 USA. [Dwyer, J. T.; Bailey, R.; Saldanha, L. G.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303114 ER PT J AU Ariza-Nieto, M Glahn, RP Ariza-Nieto, CJ Yan, J Malysheva, OV Caudill, MA AF Ariza-Nieto, Magnolia Glahn, Raymond P. Ariza-Nieto, Claudia J. Yan, Jian Malysheva, Olga V. Caudill, Marie A. TI Profiles of Betaine and Choline in Cultivars of a set of Staple Crops SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Ariza-Nieto, Magnolia; Yan, Jian; Malysheva, Olga V.; Caudill, Marie A.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Glahn, Raymond P.] USDA ARS, Ithaca, NY 14853 USA. [Ariza-Nieto, Claudia J.] CORPOICA, Mosquera, Colombia. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303891 ER PT J AU Berryman, CE West, SG Grieger, JA Blumberg, JB Kris-Etherton, PM AF Berryman, Claire E. West, Sheila G. Grieger, Jessica A. Blumberg, Jeffrey B. Kris-Etherton, Penny M. TI Effects of whole walnuts and walnut components on postprandial triglyceride response, plasma measures of antioxidant activity, and endothelial function in overweight and obese adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Berryman, Claire E.; West, Sheila G.; Grieger, Jessica A.; Kris-Etherton, Penny M.] Penn State Univ, University Pk, PA 16802 USA. [Blumberg, Jeffrey B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306090 ER PT J AU Blum-Kemelor, DM Spahn, JM Obbagy, JE Fungwe, TV Olson, SL Samuels, SE Yoshida, S Shen, T AF Blum-Kemelor, Donna Michele Spahn, Joanne M. Obbagy, Julie E. Fungwe, Thomas V. Olson, Sara L. Samuels, Sarah E. Yoshida, Sallie Shen, Ted TI Implementing the Dietary Guidelines for Americans Using New Technologies as a Channel for Nutrition Education: Evidence From Research and Best Practices SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Blum-Kemelor, Donna Michele; Spahn, Joanne M.; Obbagy, Julie E.; Fungwe, Thomas V.] USDA, CNPP EALD, Alexandria, VA USA. [Olson, Sara L.] USDA, FNS ORA, Alexandria, VA USA. [Samuels, Sarah E.; Yoshida, Sallie] Samuels & Associates, Oakland, CA USA. [Shen, Ted] IMPAQ Int LLC, Columbia, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305701 ER PT J AU Boutry, C Suryawan, A El-Kadi, SW Wheatley, SM Orellana, RA Nguyen, HV Davis, TA AF Boutry, Claire Suryawan, Agus El-Kadi, Samer W. Wheatley, Scott M. Orellana, Renan A. Nguyen, Hanh V. Davis, Teresa A. TI Leucine Pulse Increases Skeletal Muscle Protein Synthesis during Continuous Feeding in Neonatal Pigs SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Boutry, Claire; Suryawan, Agus; El-Kadi, Samer W.; Wheatley, Scott M.; Orellana, Renan A.; Nguyen, Hanh V.; Davis, Teresa A.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304819 ER PT J AU Braz, MG Braz, LG Fecchio, D Ferreira, ALD Braz, JRC Tang, GW Yeum, KJ Salvadori, DMF AF Braz, Mariana Gobbo Braz, Leandro Gobbo Fecchio, Denise dos Anjos Ferreira, Ana Lucia Cerqueira Braz, Jose Reinaldo Tang, Guangwen Yeum, Kyung-Jin Favero Salvadori, Daisy Maria TI Either Intravenous or Inhaled Anesthetic for Elective Otorhinological Surgery Does Not Induce Oxidative Stress SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Braz, Mariana Gobbo; Braz, Leandro Gobbo; Fecchio, Denise; dos Anjos Ferreira, Ana Lucia; Cerqueira Braz, Jose Reinaldo; Favero Salvadori, Daisy Maria] UNESP, Botucatu, SP, Brazil. [Tang, Guangwen; Yeum, Kyung-Jin] Tufts Univ, Jean Mayer USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300832 ER PT J AU Brito, A Allen, LH Verdugo, R Sanchez, H Hertrampf, E Albala, C Lera, L Castillo, JL Lavados, M Shahab-Ferdows, S Uauy, R AF Brito, Alex Allen, Lindsay H. Verdugo, Renato Sanchez, Hugo Hertrampf, Eva Albala, Cecilia Lera, Lydia Luis Castillo, Jose Lavados, Manuel Shahab-Ferdows, Setareh Uauy, Ricardo TI Cyanocobalamin treatment improves vitamin B12 status and peripheral neuroconduction in deficient Chilean elderly SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Brito, Alex; Sanchez, Hugo; Hertrampf, Eva; Albala, Cecilia; Lera, Lydia; Uauy, Ricardo] Univ Chile, Inst Nutr & Food Technol INTA, Santiago, Chile. [Allen, Lindsay H.; Shahab-Ferdows, Setareh] Univ Calif Davis, ARS, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA. [Verdugo, Renato; Luis Castillo, Jose; Lavados, Manuel] Univ Chile, Fac Med, Dept Neurol Sci, Santiago, Chile. [Uauy, Ricardo] London Sch Hyg & Trop Med, London WC1, England. RI Brito, Alex/I-2858-2013 OI Brito, Alex/0000-0002-6212-8814 NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306072 ER PT J AU Burri, BJ Devin, CM Rajaonary, M Roman, M La Porte, D AF Burri, Betty J. Devin, Coline M. Rajaonary, Maelle Roman, Maxine La Porte, Delphine TI No effect of pH on in vitro digestion of carotenoids from sweet potatoes and mandarin oranges SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Burri, Betty J.; Devin, Coline M.; Rajaonary, Maelle; La Porte, Delphine] ARS, USDA, WHNRC, Davis, CA USA. [Burri, Betty J.; Roman, Maxine] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303177 ER PT J AU Burrin, D Stoll, B El-Kadi, S Genovese, K Davis, T Fiorotto, M Thymann, T Sangild, P AF Burrin, Douglas Stoll, Barbara El-Kadi, Samer Genovese, Kenneth Davis, Teresa Fiorotto, Marta Thymann, Thomas Sangild, Per TI Persistence of an Adverse Metabolic Phenotype in Parenterally Fed Neonatal Pigs SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Burrin, Douglas; Stoll, Barbara; El-Kadi, Samer; Davis, Teresa; Fiorotto, Marta] Baylor Coll Med, Dept Pediat, USDA, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Genovese, Kenneth] USDA, So Plains Agr Res Ctr, College Stn, TX USA. [Thymann, Thomas; Sangild, Per] Univ Copenhagen, Dept Human Nutr, Frederiksberg C, Denmark. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301887 ER PT J AU Callihan, P Zitomer, NC Stoeling, MV Kennedy, PC Lynch, KR Riley, RT Hooks, SB AF Callihan, Phillip Zitomer, Nicholas C. Stoeling, Michael V. Kennedy, Perry C. Lynch, Kevin R. Riley, Ronald T. Hooks, Shelley B. TI Distinct generation, pharmacology, and distribution of sphingosine 1-phosphate and dihydro-sphingosine 1-phosphate in human neural progenitor cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Callihan, Phillip; Hooks, Shelley B.] Univ Georgia, Athens, GA 30602 USA. [Zitomer, Nicholas C.; Stoeling, Michael V.; Riley, Ronald T.] ARS, USDA, SAA, Athens, GA USA. [Kennedy, Perry C.; Lynch, Kevin R.] Univ Virginia, Dept Pharmacol, Charlottesville, VA 22908 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304696 ER PT J AU Campbell, C Chandler, CJ Burnett, DJ Souza, EC Casazza, GA Fiehn, O Hoppel, CL Newman, JW Garvey, WT Hunter, GR Keim, NL Fernandez, JR Adams, SH AF Campbell, Caitlin Chandler, Carol J. Burnett, Dustin J. Souza, Elaine C. Casazza, Gretchen A. Fiehn, Oliver Hoppel, Charles L. Newman, John W. Garvey, W. Timothy Hunter, Gary R. Keim, Nancy L. Fernandez, Jose R. Adams, Sean H. TI Metabolite signatures reflective of muscle metabolism and insulin sensitivity following a diet and fitness intervention in obese, sedentary, insulin-resistant women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Newman, John W.; Keim, Nancy L.; Adams, Sean H.] USDA ARS, Obes & Metab Res Unit, WHNRC, Davis, CA USA. [Casazza, Gretchen A.] Univ CA, Sports Med Program, Davis, CA USA. [Fiehn, Oliver] Univ CA, Genome Ctr, Davis, CA USA. [Hoppel, Charles L.] Case Western Reserve Univ, Cleveland, OH 44106 USA. [Garvey, W. Timothy; Hunter, Gary R.; Fernandez, Jose R.] Univ Alabama Birmingham, Birmingham, AL USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305492 ER PT J AU Cao, HP Shockey, JM Klasson, KT AF Cao, Heping Shockey, Jay M. Klasson, Kjell T. TI Expression profiles of genes coding for oil biosynthesis in developing Tung seeds SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Cao, Heping; Shockey, Jay M.; Klasson, Kjell T.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306218 ER PT J AU Cao, JJ Gregoire, BR Song, SH AF Cao, Jay J. Gregoire, Brian R. Song, Sihong TI Alpha-1 antitrypsin reduces ovariectomy-induced bone loss in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Cao, Jay J.; Gregoire, Brian R.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Song, Sihong] Univ Florida, Dept Pharmaceut, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300802 ER PT J AU Carr, N Kranz, S Jahns, LM AF Carr, Noelle Kranz, Sibylle Jahns, Lisa M. TI Applying the Dietary Guidelines for Americans to the foods actually consumed in the US: Why are so many SoFAS on MyPlate? SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Carr, Noelle; Kranz, Sibylle] Purdue Univ, W Lafayette, IN 47907 USA. [Jahns, Lisa M.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301733 ER PT J AU Charron, CS Clevidence, BA Albaugh, GP Kramer, M Vinyard, BT Milner, JA Novotny, JA AF Charron, Craig S. Clevidence, Beverly A. Albaugh, George P. Kramer, Matthew Vinyard, Bryan T. Milner, John A. Novotny, Janet A. TI Influence of consumption of allyl isothiocyanate or cabbage and mustard on DNA integrity in humans SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Charron, Craig S.; Clevidence, Beverly A.; Albaugh, George P.; Novotny, Janet A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Kramer, Matthew; Vinyard, Bryan T.] USDA, Biometr Consulting Serv, Beltsville, MD 20705 USA. [Milner, John A.] NCI, Div Canc Prevent, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305025 ER PT J AU Chen, CYO Bryant, N Blumberg, J Serra, A AF Chen, C-Y. Oliver Bryant, Nyssa Blumberg, Jeffrey Serra, Aida TI The fetal programming of dietary fructose and saturated fat on hepatic quercetin glucuronidation in rats SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Chen, C-Y. Oliver; Bryant, Nyssa; Blumberg, Jeffrey] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Serra, Aida] Univ Lleida, Lleida, Spain. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301646 ER PT J AU Chester, DN Sebastian, RS Enns, CW Goldman, JD AF Chester, Deirdra Nicole Sebastian, Rhonda S. Enns, Cecilia Wilkinson Goldman, Joseph D. TI Beverage intakes by weight status - do obese individuals drink more? Results from What We Eat in America (WWEIA), NHANES 2005-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Chester, Deirdra Nicole; Sebastian, Rhonda S.; Enns, Cecilia Wilkinson; Goldman, Joseph D.] ARS, Food Surveys Res Grp, USDA, BHNRC, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304443 ER PT J AU Claycombe, KJ Uthus, EO Johnson, WT AF Claycombe, Kate J. Uthus, Eric O. Johnson, William Thomas TI Maternal low protein diet and postnatal high fat diet increases adipose imprinted gene expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Claycombe, Kate J.; Uthus, Eric O.; Johnson, William Thomas] ARS, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301854 ER PT J AU DellaValle, DM Haas, JD Boy, E Shamah-Levy, T Villalpando, S Glahn, RP AF DellaValle, Diane M. Haas, Jere D. Boy, Erick Shamah-Levy, Teresa Villalpando, Salvador Glahn, Raymond P. TI Evaluation of a human efficacy trial: tracking biofortified iron through an intervention SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [DellaValle, Diane M.; Glahn, Raymond P.] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. [Haas, Jere D.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Boy, Erick] HarvestPlus, Washington, DC USA. [Shamah-Levy, Teresa; Villalpando, Salvador] INSP, Cuernavaca, Morelos, Mexico. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304632 ER PT J AU DellaValle, DM Glahn, RP Vandenberg, A AF DellaValle, Diane M. Glahn, Raymond P. Vandenberg, Albert TI Assessment of Iron Bioavailability in Lentils: Identifying Commercial Harvests with High Fe Bioavailability SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [DellaValle, Diane M.; Glahn, Raymond P.] ARS, USDA, Robert Holley Ctr Agr & Hlth, Ithaca, NY USA. [Vandenberg, Albert] Univ Saskatchewan, Saskatoon, SK, Canada. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301789 ER PT J AU Dwyer, JT Bailey, R Nahin, R Rogers, G Sempos, C Jacques, P AF Dwyer, Johanna T. Bailey, Regan Nahin, Richard Rogers, Gail Sempos, Christopher Jacques, Paul TI Dietary supplement (DS) use of children < 18 yr in the 2007 National Health Interview Survey (NHIS) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Dwyer, Johanna T.; Bailey, Regan; Sempos, Christopher] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Nahin, Richard] NCCAM, NIH, Bethesda, MD USA. [Rogers, Gail; Jacques, Paul] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301271 ER PT J AU Dwyer, JT Bailey, R Saldanha, L Holden, J Andrews, K Betz, J Gahche, J Hardy, C Milner, J Roseland, J AF Dwyer, Johanna T. Bailey, Regan Saldanha, Leila Holden, Joanne Andrews, Karen Betz, Joseph Gahche, Jaime Hardy, Constance Milner, John Roseland, Janet TI Progress in development of dietary supplement (DS) composition and label databases for research SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Dwyer, Johanna T.; Bailey, Regan; Saldanha, Leila; Betz, Joseph] Off Dietary Supplements, Bethesda, MD USA. [Milner, John] NCI, NIH, Bethesda, MD 20892 USA. [Holden, Joanne; Andrews, Karen; Roseland, Janet] USDA, Nutrient Data Lab, Beltsville, MD 20705 USA. [Gahche, Jaime] CDC, NCHS, Hysattsville, MD USA. [Hardy, Constance] US FDA, CFSAN, Silver Spring, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301264 ER PT J AU Ebner, RE Burke, AP Kranz, S Boushey, CJ Roberts, SB McCrory, MA AF Ebner, Rachel Eve Burke, Aoife P. Kranz, Sibylle Boushey, Carol J. Roberts, Susan B. McCrory, Megan A. TI US Trends in dietary variety and its association with BMI and micronutrient intakes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Ebner, Rachel Eve; Kranz, Sibylle; McCrory, Megan A.] Purdue Univ, W Lafayette, IN 47907 USA. [Burke, Aoife P.] Dublin Inst Technol, Sch Biol Sci, Dublin, Ireland. [Burke, Aoife P.] Trinity Coll Dublin, Dublin, Ireland. [Boushey, Carol J.] Univ Hawaii, Ctr Canc, Honolulu, HI 96822 USA. [Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305383 ER PT J AU Evans, JA Garlick, J Johnson, EJ Wang, XD Chen, CYO AF Evans, Julie A. Garlick, Jonathan Johnson, Elizabeth J. Wang, Xiang-Dong Chen, C-Y. Oliver TI The photoprotective effects of almond phytonutrients in a three-dimensional human skin tissue model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Evans, Julie A.; Johnson, Elizabeth J.; Wang, Xiang-Dong; Chen, C-Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Garlick, Jonathan] Tufts Univ, Div Canc Biol & Tissue Engn, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301675 ER PT J AU Evans, JA Garlick, J Johnson, EJ Wang, XD Chen, CYO AF Evans, Julie A. Garlick, Jonathan Johnson, Elizabeth J. Wang, Xiang-Dong Chen, C-Y. Oliver TI alpha-Tocopherol absorption into a three-dimensional human skin tissue model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Evans, Julie A.; Johnson, Elizabeth J.; Wang, Xiang-Dong; Chen, C-Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Garlick, Jonathan] Tufts Univ, Div Canc Biol & Tissue Engn, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301664 ER PT J AU Feng, L Huang, HQ Schoene, NW Yu, LL Lei, KY Wang, TTY AF Feng, Li Huang, Haiqiu Schoene, Norberta W. Yu, Liangli Lei, Kai Y. Wang, Thomas T. Y. TI Resveratrol enhances the effect of lipopolysacchride on human monocytes THP-1 pro-inflammatory cytokine expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Feng, Li; Huang, Haiqiu; Yu, Liangli; Lei, Kai Y.] Univ Maryland, College Pk, MD 20742 USA. [Schoene, Norberta W.; Wang, Thomas T. Y.] USDA, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303057 ER PT J AU Finn, AM Milliron, SE Hummer, KE Wilson, T AF Finn, Amy Marie Milliron, Sidney E. Hummer, Kim E. Wilson, Ted TI Polyphenolic and Antioxidant Properties of Elderberry (Sambucus) Fruits SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Finn, Amy Marie; Milliron, Sidney E.; Wilson, Ted] Winona State Univ, Winona, MN 55987 USA. [Hummer, Kim E.] ARS Natl Clonal Germplasm Repository, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306955 ER PT J AU Fiorotto, ML Sosa, HA Davis, TA Villegas, C Estrada, IJ AF Fiorotto, Marta L. Sosa, Horacio A. Davis, Teresa A. Villegas, Carolina Estrada, Irma J. TI Nutritionally-induced neonatal muscle growth retardation can be rescued by sustained muscle IGF-I expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Fiorotto, Marta L.; Sosa, Horacio A.; Davis, Teresa A.; Villegas, Carolina; Estrada, Irma J.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306494 ER PT J AU Forester, SM Witbracht, M Horn, W Laugero, K Keim, NL AF Forester, Shavawn M. Witbracht, Megan Horn, William Laugero, Kevin Keim, Nancy L. TI Within subject variation of satiety hormone responses to a standard lunch SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Forester, Shavawn M.; Laugero, Kevin; Keim, Nancy L.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Witbracht, Megan; Horn, William; Laugero, Kevin; Keim, Nancy L.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303103 ER PT J AU Fu, KA Kehayias, JJ AF Fu, Kristin A. Kehayias, Joseph J. TI Rapid screening for zinc deficiency using portable x-ray fluorescence in fingernails SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Fu, Kristin A.; Kehayias, Joseph J.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301587 ER PT J AU Gebauer, SK Novotny, JA Baer, DJ AF Gebauer, Sarah K. Novotny, Janet A. Baer, David J. TI Macronutrient absorption from almonds: the measured energy value of almonds in the human diet SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Gebauer, Sarah K.; Novotny, Janet A.; Baer, David J.] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711302532 ER PT J AU Gehring, R Malreddy, P Silver, K Wang, F Schultz, B AF Gehring, Ronette Malreddy, Pradeep Silver, Kristopher Wang, Florence Schultz, Bruce TI In vitro Culture alters the Expression of Xenobiotic Transporters by Mammary Epithelial Cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Gehring, Ronette; Malreddy, Pradeep; Wang, Florence; Schultz, Bruce] Kansas State Univ, Manhattan, KS 66506 USA. [Silver, Kristopher] USDA ARS, Manhattan, KS USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306697 ER PT J AU Green, A Jacques, PF Rogers, GT Meigs, JB McKeown, NM AF Green, Angela Jacques, Paul F. Rogers, Gail T. Meigs, James B. McKeown, Nicola M. TI Association between sugar-sweetened beverage consumption and the metabolically healthy obese phenotype SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Green, Angela] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Jacques, Paul F.; Rogers, Gail T.; McKeown, Nicola M.] Tufts Univ, USDA HNRCA, Boston, MA 02111 USA. [Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Meigs, James B.] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Meigs, James B.] Harvard Univ, Sch Med, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305832 ER PT J AU Green, BT Lee, ST Panter, KE AF Green, Benedict T. Lee, Stephen T. Panter, Kip E. TI A pharmacodynamic comparison of piperidine and pyridine alkaloid actions at fetal muscle-type nicotinic acetylcholine receptors (nAChR) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Green, Benedict T.; Lee, Stephen T.; Panter, Kip E.] ARS, Poisonous Plant Res Lab, USDA, NPA, Logan, UT USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300972 ER PT J AU Green, CO Badaloo, A Hsu, JW Taylor-Bryan, C Reid, M Forrester, T Jahoor, F AF Green, Curtis Omar Badaloo, Asha Hsu, Jean W. Taylor-Bryan, Carolyn Reid, Marvin Forrester, Terrence Jahoor, Farook TI Effects of methionine supplementation on cysteine and glutathione production in malnourished infants SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Green, Curtis Omar; Badaloo, Asha; Taylor-Bryan, Carolyn; Reid, Marvin; Forrester, Terrence] Univ W Indies, Trop Metab Res Unit, Kingston 7, Jamaica. [Hsu, Jean W.; Jahoor, Farook] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304817 ER PT J AU Guenther, PM O'Connell, KM Reedy, J Kirkpatrick, SI Hiza, HAB Kuczynski, KJ AF Guenther, Patricia M. O'Connell, Kellie M. Reedy, Jill Kirkpatrick, Sharon I. Hiza, Hazel A. B. Kuczynski, Kevin J. TI Development of the Healthy Eating Index-2010 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Guenther, Patricia M.; O'Connell, Kellie M.; Hiza, Hazel A. B.; Kuczynski, Kevin J.] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. [Reedy, Jill; Kirkpatrick, Sharon I.] NCI, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301470 ER PT J AU Hampel, D Allen, LH AF Hampel, Daniela Allen, Lindsay H. TI Simultaneous Analysis of B-Vitamins in Human Milk SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Hampel, Daniela; Allen, Lindsay H.] USDA ARS, WHNRC, Davis, CA USA. RI Hampel, Daniela/K-2047-2014 OI Hampel, Daniela/0000-0003-0288-7680 NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305068 ER PT J AU Haskell, MJ Jamil, KM Jamil, M Peerson, JM Keenan, AH Newman, JW Brown, KH AF Haskell, Marjorie J. Jamil, Kazi M. Jamil, Maleka Peerson, Janet M. Keenan, Alison H. Newman, John W. Brown, Kenneth H. TI Daily consumption of orange-fleshed sweet potato increased plasma beta-carotene concentration but did not increase total body vitamin A pool size in Bangladeshi women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Haskell, Marjorie J.; Peerson, Janet M.; Brown, Kenneth H.] Univ Calif Davis, Prog Intl Nutr, Davis, CA 95616 USA. [Jamil, Kazi M.; Jamil, Maleka] Intl Ctr Diarrhoeal Dis Res, Dhaka, Bangladesh. [Keenan, Alison H.; Newman, John W.] USDA Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301626 ER PT J AU Hawkes, WC Alkan, Z AF Hawkes, Wayne Chris Alkan, Zeynep TI Selenoprotein W is required for epidermal growth factor (EGF)-stimulated proliferation via a pathway involving MKK4 and p53 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Hawkes, Wayne Chris; Alkan, Zeynep] USDA, WHNRC, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301706 ER PT J AU Histed, A Lee, S Du, XG Wu, DY Meydani, SN AF Histed, Alexander Lee, Sanghan Du, Xiaogang Wu, Dayong Meydani, Simin Nikbin TI Age Modulates Effect of Fish Oil on the Immune Response in an Ovalbumin Asthmatic Murine Model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Histed, Alexander; Lee, Sanghan; Du, Xiaogang; Wu, Dayong; Meydani, Simin Nikbin] Tufts Univ, HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305285 ER PT J AU Hoy, MK Clemens, J Rhodes, DG Moshfegh, AJ AF Hoy, M. Katherine Clemens, John Rhodes, Donna G. Moshfegh, Alanna J. TI Contribution of food categories to energy intake of the US population: What We Eat In America, NHANES 2007-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Hoy, M. Katherine; Clemens, John; Rhodes, Donna G.; Moshfegh, Alanna J.] ARS, FSRG, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304439 ER PT J AU Hruby, A Ngwa, JS Meigs, JB Nettleton, JA McKeown, NM AF Hruby, Adela Ngwa, Julius S. Meigs, James B. Nettleton, Jennifer A. McKeown, Nicola M. CA CHARGE Nutr Working Grp TI Meta-analysis of interaction between dietary magnesium intake and genetic risk variants on diabetes phenotypes in the CHARGE Consortium SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Hruby, Adela; McKeown, Nicola M.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Hruby, Adela; McKeown, Nicola M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ngwa, Julius S.] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. [Meigs, James B.] Massachusetts Gen Hosp, Dept Med, Div Gen Med, Boston, MA 02114 USA. [Meigs, James B.] Harvard Univ, Sch Med, Boston, MA USA. [Nettleton, Jennifer A.; CHARGE Nutr Working Grp] Univ Texas Hlth Sci Ctr Houston, Sch Publ Hlth, Div Epidemiol Human Genet & Environm Sci, Houston, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300935 ER PT J AU Hsu, JW Badaloo, A Taylor-Bryan, C Reid, M Forrester, T Jahoor, F AF Hsu, Jean W. Badaloo, Asha Taylor-Bryan, Carolyn Reid, Marvin Forrester, Terrence Jahoor, Farook TI Dietary supplementation with aromatic amino acids improves net protein synthesis in children with severe acute malnutrition during hospitalization SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Hsu, Jean W.; Jahoor, Farook] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. [Badaloo, Asha; Taylor-Bryan, Carolyn; Reid, Marvin; Forrester, Terrence] Univ W Indies, Res Inst Trop Med, Kingston 7, Jamaica. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301404 ER PT J AU Huang, HQ Xie, ZH Yokoyama, W Boue, SM Yu, LL Wang, TTY AF Huang, Haiqiu Xie, Zhuohong Yokoyama, Wallace Boue, Stephen M. Yu, Liangli Wang, Thomas T. Y. TI Cholesterol lowering activity of soy-derived glyceollins in golden hamster model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Huang, Haiqiu; Xie, Zhuohong; Yu, Liangli] Univ Maryland, College Pk, MD 20742 USA. [Yokoyama, Wallace] ARS, USDA, Albany, CA USA. [Boue, Stephen M.] ARS, USDA, New Orleans, LA USA. [Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304481 ER PT J AU Hwang, D Huang, SR Rutkowsky, JM Snodgrass, RG Ono-Moore, KD Schneider, DA Newman, JW Adams, SH AF Hwang, Daniel Huang, Shurong Rutkowsky, Jennifer M. Snodgrass, Ryan G. Ono-Moore, Kikumi D. Schneider, Dina A. Newman, John W. Adams, Sean H. TI Saturated fatty acids activate TLR-mediated proinflammatory signaling pathways SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Hwang, Daniel; Huang, Shurong; Newman, John W.; Adams, Sean H.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Hwang, Daniel; Rutkowsky, Jennifer M.; Snodgrass, Ryan G.; Ono-Moore, Kikumi D.; Schneider, Dina A.; Newman, John W.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301932 ER PT J AU Jackson, MI Gabel-Jensen, C Lunge, K Gammelgaard, B Combs, GF AF Jackson, Matthew I. Gabel-Jensen, Charlotte Lunge, Kristoffer Gammelgaard, Bente Combs, Gerald F. TI Metabolic production of methylated selenium species requires adequate methylation status SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jackson, Matthew I.; Combs, Gerald F.] USDA ARS, Grand Forks, ND USA. [Gabel-Jensen, Charlotte; Lunge, Kristoffer; Gammelgaard, Bente] Univ Copenhagen, Copenhagen, Denmark. RI Gammelgaard, Bente/A-7826-2013 NR 0 TC 0 Z9 0 U1 0 U2 10 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711302671 ER PT J AU Jahns, L Whigham, L Johnson, L Mayne, S Cartmel, B Ermakov, I Gellermann, W AF Jahns, Lisa Whigham, Leah Johnson, Luann Mayne, Susan Cartmel, Brenda Ermakov, Igor Gellermann, Werner TI Dermal Carotenoids as Measured by Resonance Raman Spectroscopy as a Biomarker of Response to a Fruit/Vegetable Intervention Study SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jahns, Lisa; Whigham, Leah; Johnson, Luann] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Mayne, Susan; Cartmel, Brenda] Yale Univ, Sch Publ Hlth, New Haven, CT USA. [Ermakov, Igor; Gellermann, Werner] Univ Utah, Dept Phys & Astron, Salt Lake City, UT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303087 ER PT J AU Jahns, L Wadsworth, A Morin, C McDonald, LR AF Jahns, Lisa Wadsworth, Ann Morin, Charlie McDonald, Leander R. TI Understanding American-Indian Children's Perceived Barriers and Facilitators to Following the Dietary Guidelines for Americans (DGA) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jahns, Lisa] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Wadsworth, Ann; McDonald, Leander R.] Cankdeska Cikana Community Coll, Ft Totten, ND USA. [Morin, Charlie] Four Winds Community Sch, Ft Totten, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301311 ER PT J AU Jang, S Molokin, A Laskman, S Panickar, K Bruna, G Urban, JF Solano-Aguilar, G AF Jang, Saebyeol Molokin, Aleksey Laskman, Sukla Panickar, Kiran Bruna, Gonzalo Urban, Joseph F., Jr. Solano-Aguilar, Gloria TI Influence of Fructose Consumption on Inflammatory Gene Expression in Liver and Brain in a Pig Model of Juvenile Obesity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jang, Saebyeol; Molokin, Aleksey; Laskman, Sukla; Panickar, Kiran; Urban, Joseph F., Jr.; Solano-Aguilar, Gloria] ARS, Human Nutr Res Ctr, Diet Genom & Immunol Lab, USDA, Beltsville, MD USA. [Bruna, Gonzalo] Seysa, Toledo, Spain. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305876 ER PT J AU Jorgensen, JM Yang, ZY Lonnerdal, B Chantry, CJ Allen, LH Dewey, KG AF Jorgensen, Josh M. Yang, Zhenyu Lonnerdal, Bo Chantry, Caroline J. Allen, Lindsay H. Dewey, Kathryn G. TI Increased BMI is associated with lower iron status and increased inflammation and oxidative stress in postpartum women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jorgensen, Josh M.; Yang, Zhenyu; Lonnerdal, Bo; Dewey, Kathryn G.] Univ Calif Davis, Dept Nutr, Davis, CA USA. [Chantry, Caroline J.] Univ Calif Davis, Dept Pediat, Sacramento, CA USA. [Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301347 ER PT J AU Jorgensen, JM Yang, ZY Lonnerdal, B Chantry, CJ Allen, LH Dewey, KG AF Jorgensen, Josh M. Yang, Zhenyu Lonnerdal, Bo Chantry, Caroline J. Allen, Lindsay H. Dewey, Kathryn G. TI Inflammation in postpartum women is inversely related to transferrin saturation, but is not correlated with ferritin or hepcidin SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jorgensen, Josh M.; Lonnerdal, Bo; Dewey, Kathryn G.] Univ Calif Davis, Dept Nutr, Davis, CA USA. [Yang, Zhenyu] China CDC, Natl Inst Nutr & Food Safety, Beijing, Peoples R China. [Chantry, Caroline J.] Univ Calif Davis, Med Ctr, Dept Pediat, Sacramento, CA USA. [Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301340 ER PT J AU Jorgensen, JM Yang, ZY Lonnerdal, B Chantry, CJ Allen, LH Dewey, KG AF Jorgensen, Josh M. Yang, Zhenyu Lonnerdal, Bo Chantry, Caroline J. Allen, Lindsay H. Dewey, Kathryn G. TI Iron supplementation during lactation increases hemoglobin without an increase in iron status or oxidative stress SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Jorgensen, Josh M.; Lonnerdal, Bo; Dewey, Kathryn G.] Univ Calif Davis, Dept Nutr, Davis, CA USA. [Yang, Zhenyu] China CDC, Natl Inst Nutr & Food Safety, Beijing, Peoples R China. [Chantry, Caroline J.] UC Davis Med Ctr, Dept Pediat, Sacramento, CA USA. [Allen, Lindsay H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301287 ER PT J AU Karl, JP Roberts, SB AF Karl, J. Philip Roberts, Susan B. TI A review of dietary energy density and obesity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Karl, J. Philip; Roberts, Susan B.] Tufts Univ, Energy Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 2 U2 6 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300136 ER PT J AU Kim, EK Kim, YS Yu, LL Milner, JA Wang, TTY AF Kim, Eun-Kyung Kim, Young S. Yu, Liangli Milner, John A. Wang, Thomas T. Y. TI Broccoli-derived compounds modulate androgen up-regulation of C-C chemokine ligand 2 and enhancement of monocyte attraction to prostate cancer cells SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Kim, Eun-Kyung; Yu, Liangli] Univ Maryland, College Pk, MD 20742 USA. [Kim, Young S.; Milner, John A.] NCI, Nutr Sci Res Grp, Rockville, MD USA. [Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304484 ER PT J AU Kim, H Bartley, G Young, SA Yokoyama, W AF Kim, Hyunsook Bartley, Glenn Young, Scott A. Yokoyama, Wallace TI HPMC supplementation reduces weight gain, intestinal permeability, inflammation, and insulin resistance in diet-induced obese mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Kim, Hyunsook] Univ Calif Davis, Davis, CA 95616 USA. [Bartley, Glenn; Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. [Young, Scott A.] Dow Chem Co USA, Analyt Sci, Midland, MI 48674 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304918 ER PT J AU Krishnan, S Gustafson, M Campbell, C Souza, E Keim, NL AF Krishnan, Sridevi Gustafson, Mary Campbell, Caitlin Souza, Elaine Keim, Nancy L. TI Increased dietary saturated fat intake is negatively associated with compliance to exercise regimen and improvement in fitness parameters. SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Krishnan, Sridevi; Campbell, Caitlin; Keim, Nancy L.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Gustafson, Mary; Keim, Nancy L.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Souza, Elaine] Univ Calif Davis, UCDMC, Sacramento, CA 95817 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306522 ER PT J AU Lee, Y Boushey, CJ McCabe, LD McCabe, GP Novotny, R Van Loan, M Read, M Going, S Weaver, CM Savaiano, DA AF Lee, Yujin Boushey, Carol J. McCabe, Linda D. McCabe, George P. Novotny, Rachel Van Loan, Marta Read, Marsha Going, Scott Weaver, Connie M. Savaiano, Dennis A. CA ACT Investigators TI Behavioral intervention among early adolescent girls improves bone mass after 18 months; however lactose maldigestion is still a barrier for calcium intake SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Lee, Yujin; Boushey, Carol J.; McCabe, Linda D.; McCabe, George P.; Weaver, Connie M.; Savaiano, Dennis A.; ACT Investigators] Purdue Univ, W Lafayette, IN 47907 USA. [Novotny, Rachel] Univ Hawaii, Program Epidemiol, Honolulu, HI 96822 USA. [Van Loan, Marta] USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Read, Marsha] Univ Nevada, Reno, NV 89557 USA. [Going, Scott] Univ Arizona, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304754 ER PT J AU Lin, AHM Nichols, BL Hamaker, BR AF Lin, Amy Hui-Mei Nichols, Buford L. Hamaker, Bruce R. TI Concept of slowly released dietary glucose: a focus on starch digestion at the mucosal alpha-glucosidase level SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Lin, Amy Hui-Mei; Hamaker, Bruce R.] Purdue Univ, Whistler Ctr Carbohydrate Res, W Lafayette, IN 47907 USA. [Nichols, Buford L.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARG, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 1 U2 8 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304446 ER PT J AU Ma, JT Ross, A Bruce, SJ Jacques, P Lichtenstein, AH Saltzman, E Booth, S McKeown, N AF Ma, Jiantao Ross, Alastair Bruce, Stephen J. Jacques, Paul Lichtenstein, Alice H. Saltzman, Edward Booth, Sarah McKeown, Nicola TI Biomarker of whole grain wheat intake associated lower BMI in older adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Ma, Jiantao; Jacques, Paul; Lichtenstein, Alice H.; Saltzman, Edward; Booth, Sarah; McKeown, Nicola] Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA 02111 USA. [Ross, Alastair; Bruce, Stephen J.] Nestle Res Ctr, CH-1000 Lausanne, Switzerland. [Jacques, Paul; Lichtenstein, Alice H.; Saltzman, Edward; Booth, Sarah; McKeown, Nicola] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300393 ER PT J AU Mai, V Fredborg, M Ukhanova, M Wang, XY Daniel, S Novotny, J Gebauer, S Baer, D AF Mai, Volker Fredborg, Marlene Ukhanova, Maria Wang, Xiaoyu Daniel, Sara Novotny, Janet Gebauer, Sarah Baer, David TI Human gut microbiota changes after consumption of almonds or pistachios SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Mai, Volker] Univ Florida, Microbiol & Cell Sci & Emerging Pathogens Inst, Gainesville, FL USA. [Fredborg, Marlene] Univ Aarhus, Aarhus, Denmark. [Ukhanova, Maria; Wang, Xiaoyu] Univ Florida, Emerging Pathogens Inst, Gainesville, FL USA. [Novotny, Janet; Gebauer, Sarah; Baer, David] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304417 ER PT J AU Margolis, LM Cao, JJ Sauter, ER Whigham, LD McClung, JP Combs, GF Young, AJ Pasiakos, SM AF Margolis, Lee M. Cao, Jay J. Sauter, Edward R. Whigham, Leah D. McClung, James P. Combs, Gerald F. Young, Andrew J. Pasiakos, Stefan M. TI A high protein diet upregulates whole-body protein turnover during energy deficit SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Margolis, Lee M.; McClung, James P.; Young, Andrew J.; Pasiakos, Stefan M.] USA, Environm Med Res Inst, Natick, MA 01760 USA. [Cao, Jay J.; Whigham, Leah D.; Combs, Gerald F.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Sauter, Edward R.] UND Sch Med & Hlth Sci, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300225 ER PT J AU Marini, JC AF Marini, Juan C. TI Precursors for Citrulline synthesis: Facts and artifacts SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Marini, Juan C.] Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat Nutr, ARS,USDA, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300665 ER PT J AU Marquez, AP Forester, S Witbracht, M Campbell, C Gustafson, M Keim, N AF Marquez, Alexa Paulina Forester, Shavawn Witbracht, Megan Campbell, Caitlin Gustafson, Mary Keim, Nancy TI Skipping breakfast is associated with lower physical activity energy expenditure in young healthy women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Marquez, Alexa Paulina; Forester, Shavawn; Campbell, Caitlin; Keim, Nancy] Univ Calif Davis, Davis, CA 95616 USA. [Witbracht, Megan; Gustafson, Mary; Keim, Nancy] ARS, USDA, Western Human Nutr Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711302268 ER PT J AU McKay, DL Chen, CYO Rasmussen, H Blumberg, JB AF McKay, Diane L. Chen, C-Y. Oliver Rasmussen, Helen Blumberg, Jeffrey B. TI Effects of whole eggs combined with other cooked breakfast foods on postprandial metabolism in older, overweight adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [McKay, Diane L.; Chen, C-Y. Oliver; Rasmussen, Helen; Blumberg, Jeffrey B.] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer USDA, Antioxidants Res Lab, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306339 ER PT J AU McKay, DL Chen, CYO Gomez-Juaristi, M Blumberg, JB AF McKay, Diane L. Chen, C-Y. Oliver Gomez-Juaristi, Miren Blumberg, Jeffrey B. TI Bioavailability and pharmacokinetics of whole grain phytochemicals in overweight/obese adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [McKay, Diane L.; Chen, C-Y. Oliver; Blumberg, Jeffrey B.] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gomez-Juaristi, Miren] Spanish Council Sci Res CSIC, Inst Food Technol & Nutr, Madrid, Spain. NR 0 TC 0 Z9 0 U1 0 U2 6 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305840 ER PT J AU McKay, DL Chen, CYO Blumberg, JB AF McKay, Diane L. Chen, C-Y. Oliver Blumberg, Jeffrey B. TI Effects of whole grain phytochemicals on biomarkers of postprandial metabolic dysregulation in overweight/obese adults following an oral glucose challenge SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [McKay, Diane L.; Chen, C-Y. Oliver; Blumberg, Jeffrey B.] Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305779 ER PT J AU McKeown, NM Ma, YY Rogers, G Meigs, J Fontes, J Jacques, P AF McKeown, Nicola Mary Ma, YiYi Rogers, Gail Meigs, James Fontes, Joao Jacques, Paul TI Whole grain intake is associated with inflammatory markers in the Framingham Offspring Study SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [McKeown, Nicola Mary; Ma, YiYi; Rogers, Gail; Jacques, Paul] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. [McKeown, Nicola Mary; Ma, YiYi; Jacques, Paul] Gerald J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA USA. [Meigs, James] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Fontes, Joao] Boston Univ, Sch Med, Dept Cardiovasc Med, Boston, MA 02118 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711302230 ER PT J AU Monagas, M Schoene, N Urban, J Solano-Aguilar, G AF Monagas, Maria Schoene, Norberta Urban, Joseph Solano-Aguilar, Gloria TI Proanthocyanidins stimulate an inflammatory response in a pig intestinal epithelial cell line (IPEC-1 cells) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Monagas, Maria] CSIC, UAM, Inst Res Food Sci, CIAL, Madrid, Spain. [Schoene, Norberta; Urban, Joseph; Solano-Aguilar, Gloria] ARS, USDA, BHNRC, Diet Genom & Immunol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305095 ER PT J AU Nelson, SM Carlson, B Urban, J Lei, XG Hatfield, D Prabhu, KS AF Nelson, Shakira M. Carlson, Bradley Urban, Joseph Lei, Xingen Hatfield, Dolph Prabhu, K. Sandeep TI Selenoprotein induced M1 to M2 murine macrophage phenotype switching is imperative in helminth parasite clearance SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Nelson, Shakira M.; Prabhu, K. Sandeep] Penn State Univ, University Pk, PA 16802 USA. [Carlson, Bradley; Hatfield, Dolph] NCI, Bethesda, MD 20892 USA. [Urban, Joseph] USDA, Beltsville, MD 20705 USA. [Lei, Xingen] Cornell Univ, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303681 ER PT J AU Nielsen, FH AF Nielsen, Forrest Harold TI Increased adiposity induced by high dietary butter oil increases vertebrae trabecular structural indices in rats SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Nielsen, Forrest Harold] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300534 ER PT J AU Novotny, JA Chuang, JC Fadel, JG Holstege, DM Furr, HC Clifford, AJ AF Novotny, Janet A. Chuang, Jennifer C. Fadel, James G. Holstege, Dirk M. Furr, Harold C. Clifford, Andrew J. TI Quantitation of alpha-tocopherol metabolism in humans SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Novotny, Janet A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Chuang, Jennifer C.; Clifford, Andrew J.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Fadel, James G.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Holstege, Dirk M.] Univ Calif Davis, UC Davis Analyt Lab, Davis, CA 95616 USA. [Furr, Harold C.] Univ Wisconsin, Dept Nutr, Madison, WI USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306422 ER PT J AU Nwugo, CC Lin, H Duan, YP Civerolo, E AF Nwugo, Chika Charles Lin, Hong Duan, Yong-Ping Civerolo, Edwin TI The effects of 'Candidatus Liberibacter asiaticus' infection on the protein expression profiles and nutrient status of citrus plants SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Nwugo, Chika Charles; Lin, Hong; Civerolo, Edwin] USDA ARS, Parlier, CA USA. [Duan, Yong-Ping] USDA ARS, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 8 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305124 ER PT J AU O'Connell, KM Kuczynski, K Guenther, PM Rimm, EB Perez-Escamilla, R Britten, P AF O'Connell, Kellie M. Kuczynski, Kevin Guenther, Patricia M. Rimm, Eric B. Perez-Escamilla, Rafael Britten, Patricia TI Increasing seafood in the USDA Food Patterns increases eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) and other nutrients SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [O'Connell, Kellie M.; Kuczynski, Kevin; Guenther, Patricia M.; Britten, Patricia] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. [Rimm, Eric B.] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA. [Perez-Escamilla, Rafael] Yale Univ, Sch Publ Hlth, New Haven, CT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301619 ER PT J AU Olanrewaju, HA Purswell, JL Collier, SD Branton, SL AF Olanrewaju, Hammed A. Purswell, Joseph L. Collier, Stephanie D. Branton, Scott L. TI Effects of photoperiod, light intensity, and their interaction on blood physiological variables of modern broilers grown to heavy weights SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Olanrewaju, Hammed A.; Purswell, Joseph L.; Collier, Stephanie D.; Branton, Scott L.] ARS, Poultry Unit, USDA, Starkville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301094 ER PT J AU Park, LK Saltzman, E Schnitzler, G Yoon, BW Parnell, LD Lai, CQ Choi, SW AF Park, Lara Kristin Saltzman, Edward Schnitzler, Gavin Yoon, Byung Woo Parnell, Laurence D. Lai, Chao Qiang Choi, Sang-Woon TI Aging is a more significant determinant of hepatic DNA methylation patterns than a Western style diet SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Park, Lara Kristin; Saltzman, Edward; Yoon, Byung Woo; Parnell, Laurence D.; Lai, Chao Qiang; Choi, Sang-Woon] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Park, Lara Kristin; Saltzman, Edward; Choi, Sang-Woon] Gerard J & Dorothy R Friedman Sch Nutr Sci & Poli, Boston, MA USA. [Schnitzler, Gavin] Tufts Med Ctr, Mol Cardiol Res Inst, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303340 ER PT J AU Peach, CA Hussain, S Liu, C Wang, XD Greenberg, AS Mayer, J AF Peach, Camilla Anne Hussain, Sajid Liu, Chun Wang, Xiang-Dong Greenberg, Andrew S. Mayer, Jean TI Tumor progression locus 2 deletion attenuates alcohol-induced hepatic inflammatory foci and cytokine expression in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Peach, Camilla Anne; Hussain, Sajid; Liu, Chun; Wang, Xiang-Dong; Greenberg, Andrew S.; Mayer, Jean] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301593 ER PT J AU Perez-Exposito, A Hossain, B Woodhouse, LR Haskell, MJ Jamil, KM Islam, MM Peerson, JM Brown, KH AF Perez-Exposito, Ana Hossain, Bakhtiar Woodhouse, Leslie R. Haskell, Marjorie J. Jamil, Kazi M. Islam, M. Munirul Peerson, Janet M. Brown, Kenneth H. TI The addition of preformed vitamin A, but not orange-fleshed sweet potato with high beta-carotene content, to rice-based meals reduces zinc absorption in vitamin A-depleted Bangladeshi women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Perez-Exposito, Ana] Interamer Dev Bank, Panama City, Panama. [Hossain, Bakhtiar; Jamil, Kazi M.; Islam, M. Munirul] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh. [Woodhouse, Leslie R.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Haskell, Marjorie J.; Peerson, Janet M.; Brown, Kenneth H.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 6 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711307196 ER PT J AU Piccolo, BD Gertz, E Thomas, AP Keim, NL Adams, SH Seyoum, E Dolnikowski, G Van Loan, MD AF Piccolo, Brian D. Gertz, Erik Thomas, Anthony P. Keim, Nancy L. Adams, Sean H. Seyoum, Elias Dolnikowski, Gregory Van Loan, Marta D. TI Association between Vitamin D Metabolites in Fat Tissue and Serum 25-hydroxyvitamin D in Overweight and Obese Adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Piccolo, Brian D.; Thomas, Anthony P.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Gertz, Erik; Keim, Nancy L.; Adams, Sean H.; Van Loan, Marta D.] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Seyoum, Elias; Dolnikowski, Gregory] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304926 ER PT J AU Picklo, MJ Rosenberger, TA Burr, GS Wolters, WR Raatz, SK AF Picklo, Matthew J. Rosenberger, Thad A. Burr, Gary S. Wolters, William R. Raatz, Susan K. TI Twice-weekly consumption of farmed Atlantic salmon increases plasma content of phospholipid n-3 fatty acids SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Picklo, Matthew J.; Raatz, Susan K.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Rosenberger, Thad A.] Univ N Dakota, Dept Pharmacol Physiol & Therapeut, Grand Forks, ND 58201 USA. [Burr, Gary S.; Wolters, William R.] USDA ARS, Natl Cold Water Marine Aquacultrue Ctr, Franklin, ME USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301006 ER PT J AU Pieper, J Laugero, K AF Pieper, Joy Laugero, Kevin TI Relationship between Executive Function, Mood, and Eating Behavior in Preschoolers SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Pieper, Joy; Laugero, Kevin] Univ Calif Davis, Davis, CA 95616 USA. [Laugero, Kevin] USDA, WHNRC, Davis, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 8 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301022 ER PT J AU Potter, VJV Strissel, K Obin, MS Greenberg, AS AF Potter, Victoria Jeanne Vieira Strissel, Katherine Obin, Martin S. Greenberg, Andrew S. TI Although Equally Obese, High Fat Diet (HF)-Fed Ovariectomized (O) Mice Respond with Greater Adipose Tissue (AT) Inflammation Than Sham-Operated (S) Mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Potter, Victoria Jeanne Vieira; Strissel, Katherine; Obin, Martin S.; Greenberg, Andrew S.] Tufts Univ, USDA, HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305018 ER PT J AU Poulose, SM Bielinski, DF Gomes, SM Carrihill-Knoll, K Rabin, BM Shukitt-Hale, B AF Poulose, Shibu M. Bielinski, Donna F. Gomes, Stacey M. Carrihill-Knoll, Kirsty Rabin, Bernard M. Shukitt-Hale, Barbara TI Protective effects of berries and walnuts against the accelerated aging and age-associated stress caused by irradiation in critical regions of rat brain SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Poulose, Shibu M.; Gomes, Stacey M.; Shukitt-Hale, Barbara] Tufts Univ, USDA, ARS, HNRCA, Boston, MA 02111 USA. [Carrihill-Knoll, Kirsty; Rabin, Bernard M.] Univ Maryland Baltimore Cty, Dept Psychol, Baltimore, MD 21228 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305581 ER PT J AU Qin, BL Canini, F Roussel, AM Anderson, RA AF Qin, Bolin Canini, Frederic Roussel, Anne Marie Anderson, Richard A. TI Cinnamon counteracts the negative effects of a high fat/high fructose diet on brain insulin signaling and behavior SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Qin, Bolin; Anderson, Richard A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Qin, Bolin] Integr Nutraceut Int, Springhill, TN USA. [Canini, Frederic] CRSSA Army Res Ctr Hlth, Grenoble, France. [Anderson, Richard A.] Univ Grenoble 1, LBFA INSERM 884, Grenoble, France. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304490 ER PT J AU Qin, BL Polansky, MM Anderson, RA AF Qin, Bolin Polansky, Marilyn M. Anderson, Richard A. TI Cinnamon polyphenols regulate high-fructose feeding and age-induced decreases in sirtuins in rat intestinal enterocytes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Qin, Bolin; Polansky, Marilyn M.; Anderson, Richard A.] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Qin, Bolin] Integr Nutraceut Int, Springhill, TN USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303636 ER PT J AU Raatz, SK Johnson, LK Scheet, AJ Langei, CM Westereng, RA AF Raatz, Susan K. Johnson, Luann K. Scheet, Angela J. Langei, Christine M. Westereng, Rebecca A. TI Community based lifestyle intervention improves body weight, anthropometric, and fitness parameters SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Raatz, Susan K.; Johnson, Luann K.; Scheet, Angela J.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Langei, Christine M.] Ctr Court Fitness Ctr, Grand Forks, ND USA. [Westereng, Rebecca A.] Altru Hlth Syst, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300647 ER PT J AU Rhodes, DG Clemens, JC Adler, ME Moshfegh, AJ AF Rhodes, Donna G. Clemens, John C. Adler, Meghan E. Moshfegh, Alanna J. TI Nutrient intakes from restaurants: What We Eat in America, 2007-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Rhodes, Donna G.; Clemens, John C.; Adler, Meghan E.; Moshfegh, Alanna J.] USDA ARS, Food Surveys Res Grp, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303574 ER PT J AU Roe, AJ Scott, TM Chen, CYO Vishwanathan, R Eggert, EMR Schalch, W Wittwer, J Johnson, EJ AF Roe, Annie J. Scott, Tammy M. Chen, Chung-Yen O. Vishwanathan, Rohini Eggert, Emily M. R. Schalch, Wolfgang Wittwer, Jonas Johnson, Elizabeth J. TI Carotenoids and lipid peroxidation in human brains with and without Alzheimer's disease SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Roe, Annie J.; Scott, Tammy M.; Chen, Chung-Yen O.; Vishwanathan, Rohini; Eggert, Emily M. R.; Johnson, Elizabeth J.] Tufts Univ, HNRCA, Boston, MA 02111 USA. [Schalch, Wolfgang; Wittwer, Jonas] DSM, Basel, Switzerland. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305892 ER PT J AU Saldanha, LG Dwyer, JT Holden, JM Andrews, KW Bailey, RL Betz, JM Gahche, JJ Hardy, CJ Milner, J Roseland, JM AF Saldanha, Leila G. Dwyer, Johanna T. Holden, Joanne M. Andrews, Karen W. Bailey, Regan L. Betz, Joseph M. Gahche, Jaime J. Hardy, Constance J. Milner, John Roseland, Janet M. TI Identifying non-vitamin & mineral bioactive (non-VM) ingredients for inclusion in Dietary Supplement (DS) Composition Databases SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Milner, John] NCI, NIH, Bethesda, MD 20892 USA. [Saldanha, Leila G.; Dwyer, Johanna T.; Bailey, Regan L.; Betz, Joseph M.] Off Dietary Supplements, Bethesda, MD USA. [Holden, Joanne M.; Andrews, Karen W.; Roseland, Janet M.] ARS, USDA, Beltsville, MD USA. [Gahche, Jaime J.] CDC, NHANES, NCHS, Hyattsville, MD USA. [Hardy, Constance J.] US FDA, CFSAN, College Pk, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711302966 ER PT J AU Salinardi, T Batra, P Das, SK Robinson, L Lichtenstein, AH Deckersbach, T Roberts, S AF Salinardi, Taylor Batra, Payal Das, Sai Krupa Robinson, Lisa Lichtenstein, Alice H. Deckersbach, Thilo Roberts, Susan TI A Worksite-based Weight Loss Intervention for Obesity Prevention SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Salinardi, Taylor; Batra, Payal; Das, Sai Krupa; Robinson, Lisa; Roberts, Susan] John Mayer USDA, Energy Metab Lab, Human Nutr Res Ctr Aging, Boston, MA USA. [Lichtenstein, Alice H.] John Mayer USDA, Cardiovasc Nutr Lab, Human Nutr Res Ctr Aging, Boston, MA USA. [Deckersbach, Thilo] Massachusetts Gen Hosp, Bipolar Clin, Boston, MA 02114 USA. [Deckersbach, Thilo] Massachusetts Gen Hosp, Res Program, Boston, MA 02114 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304797 ER PT J AU Schaefer, SE Van Loan, M Allen, L German, JB AF Schaefer, Sara Elizabeth Van Loan, Marta Allen, Lindsay German, John Bruce TI Feasibility of accelerometers for measuring children's daily physical activity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Schaefer, Sara Elizabeth; German, John Bruce] Univ Calif Davis, Davis, CA 95616 USA. [Schaefer, Sara Elizabeth; German, John Bruce] Foods Hlth Inst, Davis, CA USA. [Van Loan, Marta; Allen, Lindsay] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306498 ER PT J AU Schroeder, N Rushovich, B Bartlett, E Gittelsohn, J Caballero, B AF Schroeder, Natalia Rushovich, Berenice Bartlett, Edward Gittelsohn, Joel Caballero, Benjamin TI Early Obesity Prevention: A Randomized Trial of a Practice-Based Intervention in 0-24 Months Infants SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Schroeder, Natalia] Johns Hopkins Univ, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Rushovich, Berenice] Univ Maryland, Sch Social Work, Baltimore, MD 21201 USA. [Bartlett, Edward] Johns Hopkins Med Inst, Baltimore, MD 21205 USA. [Gittelsohn, Joel; Caballero, Benjamin] Johns Hopkins Univ, Program Human Nutr, Baltimore, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303032 ER PT J AU Sebastian, R Enns, CW Goldman, J Zizza, C AF Sebastian, Rhonda Enns, Cecilia Wilkinson Goldman, Joseph Zizza, Claire TI Beverage choices and contributions to food and nutrient intakes differ by smoking status: Results from What We Eat in America (WWEIA), NHANES 2005-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Sebastian, Rhonda; Enns, Cecilia Wilkinson; Goldman, Joseph] USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD 20705 USA. [Zizza, Claire] Auburn Univ, Coll Human Sci, Dept Nutr Dietet & Hospitality Management, Auburn, AL 36849 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711304288 ER PT J AU Shahab-Ferdows, S Engle-Stone, R Nankap, M Ndjiebayi, AO Brown, KH Allen, LH AF Shahab-Ferdows, Setareh Engle-Stone, Reina Nankap, Martin Ndjiebayi, Alex O. Brown, Kenneth H. Allen, Lindsay H. TI Prevalence of low B12 and folate status of Cameroonian women and children, and risk factors for deficiency SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Shahab-Ferdows, Setareh; Allen, Lindsay H.] ARS, WHNRC, USDA, Davis, CA USA. [Engle-Stone, Reina; Brown, Kenneth H.; Allen, Lindsay H.] Univ Calif Davis, Davis, CA 95616 USA. [Nankap, Martin; Ndjiebayi, Alex O.; Brown, Kenneth H.] Helen Keller Int, Yaounde, Cameroon. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306511 ER PT J AU Sharma, RS Roberts, SB Polissar, NL Boushey, CJ Maroney, OH Fieselmann, KN Ebner, RE Fuss, PJ McCrory, MA AF Sharma, Rashmi S. Roberts, Susan B. Polissar, Nayak L. Boushey, Carol J. Maroney, Owen H. Fieselmann, Krista N. Ebner, Rachel E. Fuss, Paul J. McCrory, Megan A. TI Urinary sugars (sucrose and fructose) associations with self-reported sugars intake: the influence of plausibility of reported energy intake SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Sharma, Rashmi S.; McCrory, Megan A.] Purdue Univ, IBRC, W Lafayette, IN 47907 USA. [Roberts, Susan B.; Fuss, Paul J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Polissar, Nayak L.] Mt Whisper Light Stat, Seattle, WA USA. [Boushey, Carol J.] Univ Hawaii, Ctr Canc, Honolulu, HI 96822 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711307117 ER PT J AU Shen, CL Cao, JJ Dagda, RY Chyu, MC Wang, JS AF Shen, Chwan-Li Cao, Jay J. Dagda, Raul Y. Chyu, Ming-Chien Wang, Jia-Sheng TI Green tea polyphenols benefit bone health in obese female rats fed with high-fat and restricted diets SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Shen, Chwan-Li] Texas Tech Univ, Hlth Sci Ctr, LWBIWH, Lubbock, TX 79430 USA. [Cao, Jay J.] USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Wang, Jia-Sheng] Univ Georgia, Athens, GA 30602 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300351 ER PT J AU Snodgrass, RG Huang, SR Choi, IW Rutledge, JC Hwang, D AF Snodgrass, Ryan G. Huang, Shurong Choi, Il-Whan Rutledge, John C. Hwang, Daniel TI Palmitic acid induces but docosahexaenoic acid (DHA) inhibits inflammasome-mediated secretion of IL-1 beta in monocytes by modulating the activation of Toll-like receptor 2 (TLR2) SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Snodgrass, Ryan G.; Hwang, Daniel] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Huang, Shurong; Choi, Il-Whan; Hwang, Daniel] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. [Rutledge, John C.] Univ Calif, Dept Internal Med, Sacramento, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303721 ER PT J AU Solano-Aguilar, GI Jang, S Laskman, S Molokin, A Bruna, G Mitchell, A Urban, J AF Solano-Aguilar, Gloria Isabel Jang, Saebyeol Laskman, Sukla Molokin, Aleksey Bruna, Gonzalo Mitchell, Alva Urban, Joseph TI Differential Effects of Added Dietary Fructose or/and Fat on Adipose Tissue Gene Expression in a Juvenile Pig Obesity Model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Solano-Aguilar, Gloria Isabel; Jang, Saebyeol; Laskman, Sukla; Molokin, Aleksey; Urban, Joseph] USDA ARS, BHNRC, Beltsville, MD USA. [Bruna, Gonzalo] Seysa, Toledo, Spain. [Mitchell, Alva] USDA ARS, ANRI, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305797 ER PT J AU Stephensen, CB Zerofsky, M Burnett, DJ Lin, YP Hammock, BD Hall, LM McHugh, T AF Stephensen, Charles B. Zerofsky, Melissa Burnett, Dustin J. Lin, Yanping Hammock, Bruce D. Hall, Laura M. McHugh, Tara TI Vitamin D2 intake increases 25-hydroxy vitamin D2 but decreases 25-hydroxy vitamin D3 concentration in the serum of healthy adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Stephensen, Charles B.; Zerofsky, Melissa; Burnett, Dustin J.] USDA WHNRC, Davis, CA USA. [Zerofsky, Melissa; Burnett, Dustin J.; Lin, Yanping; Hammock, Bruce D.] Univ Calif Davis, Davis, CA 95616 USA. [Hall, Laura M.] Calif Polytech State Univ San Luis Obispo, San Luis Obispo, CA 93407 USA. [McHugh, Tara] USDA WRRC, Albany, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301120 ER PT J AU Stewart, CP Shahab-Ferdows, S Chen, FYJ Lindsay, AH Lonnerdal, B King, JC AF Stewart, Christine P. Shahab-Ferdows, Setti Chen, Frances Y. J. Lindsay, Allen H. Lonnerdal, Bo King, Janet C. TI Associations among adiposity, inflammation, and iron status in overweight/obese pregnant women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Stewart, Christine P.; Chen, Frances Y. J.; Lindsay, Allen H.; Lonnerdal, Bo; King, Janet C.] Univ Calif Davis, Davis, CA USA. [Shahab-Ferdows, Setti; Lindsay, Allen H.] ARS, USDA, WHNRC, Davis, CA USA. [King, Janet C.] CHORI, Oakland, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303490 ER PT J AU Suryawan, A Orellana, RA Nguyen, HV Almonaci, RD Davis, TA AF Suryawan, Agus Orellana, Renan A. Nguyen, Hanh V. Almonaci, Rosemarie D. Davis, Teresa A. TI Amino acids suppress the autophagic degradation pathway in skeletal muscle of septic neonatal pigs SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Suryawan, Agus; Orellana, Renan A.; Nguyen, Hanh V.; Almonaci, Rosemarie D.; Davis, Teresa A.] Baylor Coll Med, Childrens Nutr Res Ctr, Dept Pediat, USDA ARS, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305022 ER PT J AU Tako, E Hoekenga, OA Kochian, LV Glahn, RP AF Tako, Elad Hoekenga, Owen A. Kochian, Leon V. Glahn, Raymond P. TI Biofortified maize (Zea mays L.) provides more bioavailable iron than standard maize: Studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Tako, Elad; Hoekenga, Owen A.; Kochian, Leon V.; Glahn, Raymond P.] Cornell Univ, USDA, ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301350 ER PT J AU Tako, E Blair, MW Glahn, RP AF Tako, Elad Blair, Matthew W. Glahn, Raymond P. TI Biofortified red mottled beans (Phaseolus vulgaris L) in a maize and bean diet provide more bioavailable iron than standard red mottled beans: Studies in poultry (Gallus gallus) and an in vitro digestion/Caco 2 model SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Tako, Elad; Glahn, Raymond P.] Cornell Univ, USDA, ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301337 ER PT J AU Traber, MG Leonard, SW Fu, XY Grusak, MA Booth, S AF Traber, Maret G. Leonard, Scott W. Fu, Xueyan Grusak, Michael A. Booth, Sarah TI Plasma alpha-tocopherol transport studied using deuterium-labeled collard greens SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Traber, Maret G.; Leonard, Scott W.] Oregon State Univ, Linus Pauling Inst, Corvallis, OR 97331 USA. [Fu, Xueyan; Booth, Sarah] USDA HNRCA Tufts, Boston, MA USA. [Grusak, Michael A.] USDA ARS Childrens Nutr Res Ctr, Houston, TX USA. [Grusak, Michael A.] Baylor Coll Med, Dept Pediat, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711306646 ER PT J AU Troy, LM Jacques, PF AF Troy, Lisa M. Jacques, Paul F. TI Diets that follow the 2010 Dietary Guidelines for Americans (DGA) are associated with higher intakes of nutrients of concern SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Troy, Lisa M.; Jacques, Paul F.] Tufts Univ, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Troy, Lisa M.; Jacques, Paul F.] Tufts Univ, Friedman Sch, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303954 ER PT J AU Uthus, EO Claycombe, KJ Johnson, WT AF Uthus, Eric O. Claycombe, Kate J. Johnson, William Thomas TI Effects of pre- and postnatal diets on body compositions of diet-induced obesity prone Sprague-Dawley rats SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Uthus, Eric O.; Claycombe, Kate J.; Johnson, William Thomas] USDA ARS, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711302817 ER PT J AU Vishwanathan, R Wittwer, J Schalch, W Johnson, EJ AF Vishwanathan, Rohini Wittwer, Jonas Schalch, Wolfgang Johnson, Elizabeth J. TI Relationship between brain lutein (L) and zeaxanthin (Z) and retinal L and Z in humans SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Vishwanathan, Rohini; Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Wittwer, Jonas; Schalch, Wolfgang] DSM Nutr Prod Ltd, Kaiseraugst, Switzerland. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303366 ER PT J AU Walzem, RL Dowd, SE Thomas, AP Dunn, TN Adams, SH Pillai, SD AF Walzem, Rosemary L. Dowd, Scot E. Thomas, Anthony P. Dunn, Tamara N. Adams, Sean H. Pillai, Suresh D. TI Calcium effects on bodyweight appear to be mediated in part by changes in gut microflora and altered by dietary matrix SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Walzem, Rosemary L.] Texas A&M Univ, Fac Nutr, College Stn, TX USA. [Pillai, Suresh D.] Texas A&M Univ, Fac Food Sci, College Stn, TX USA. [Dowd, Scot E.] Res & Testing Lab, Lubbock, TX USA. [Thomas, Anthony P.; Dunn, Tamara N.; Adams, Sean H.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Thomas, Anthony P.; Dunn, Tamara N.; Adams, Sean H.] USDA, WHNRC, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305902 ER PT J AU Wright, ML Anderson, CM Uthus, EO Ohm, JE AF Wright, Michelle L. Anderson, Cindy M. Uthus, Eric O. Ohm, Joyce E. TI DNA methylation in candidate genes as a biomarker for transgenerational risk of preeclampsia SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Wright, Michelle L.; Anderson, Cindy M.] Univ N Dakota, Sch Med & Hlth Sci, Coll Nursing, Grand Forks, ND 58201 USA. [Ohm, Joyce E.] Univ N Dakota, Sch Med & Hlth Sci, Dept Biochem & Mol Biol, Grand Forks, ND 58201 USA. [Uthus, Eric O.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305257 ER PT J AU Wu, XZ Smith, SB Crouse, SF Pedersen, TL Newman, JW Walzem, RL AF Wu, Xiuzhi Smith, Stephen B. Crouse, Stephen F. Pedersen, Theresa L. Newman, John W. Walzem, Rosemary L. TI Ground beef consumption and MUFA:SAT alters HDL oxylipin profile in healthy men SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Wu, Xiuzhi; Smith, Stephen B.; Crouse, Stephen F.; Walzem, Rosemary L.] Texas A&M Univ, Intercoll Fac Nutr, College Stn, TX USA. [Pedersen, Theresa L.; Newman, John W.] USDA Western Human Nutr Res Ctr, Davis, CA USA. [Newman, John W.] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303670 ER PT J AU Yan, L Demars, LC AF Yan, Lin Demars, Lana C. TI Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Yan, Lin; Demars, Lana C.] USDA ARS, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711305752 ER PT J AU Zampariello, CA Mckay, DL Dolnikowski, G Blumberg, J Chen, CYO AF Zampariello, Carly A. Mckay, Diane L. Dolnikowski, Gregory Blumberg, Jeffrey Chen, C-Y. Oliver TI Determination of cranberry proanthocyanidin A2 in human plasma and urine using LC-MS/MS SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Zampariello, Carly A.; Mckay, Diane L.; Dolnikowski, Gregory; Blumberg, Jeffrey; Chen, C-Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 12 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301740 ER PT J AU Zanovec, M O'Neil, CE Fulgoni, VL Nicklas, TA AF Zanovec, Michael O'Neil, Carol E. Fulgoni, Victor L., III Nicklas, Theresa A. TI Dietary protein is inversely associated with sarcopenia, sarcopenic-obesity, and impaired gait speed in US adults: NHANES 1999-2002 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Zanovec, Michael; O'Neil, Carol E.] LSU AgCtr, Sch Human Ecol, Baton Rouge, LA USA. [Fulgoni, Victor L., III] Nutr Impact LLC, Battle Creek, MI USA. [Nicklas, Theresa A.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Houtson, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711303333 ER PT J AU Zingg, JM Hasan, ST Cowan, D Ricciarelli, R Azzi, A Meydani, M AF Zingg, Jean-Marc Hasan, Syeda Tahira Cowan, Danica Ricciarelli, Roberta Azzi, Angelo Meydani, Mohsen TI Regulatory effects of curcumin on FOXO3a in THP-1 monocytes/macrophages SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Zingg, Jean-Marc; Hasan, Syeda Tahira; Cowan, Danica; Azzi, Angelo; Meydani, Mohsen] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. [Ricciarelli, Roberta] Univ Genoa, DIMES, Genoa, Italy. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300171 ER PT J AU Zingg, JM Meydani, M Azzi, A AF Zingg, Jean-Marc Meydani, Mohsen Azzi, Angelo TI alpha-Tocopheryl phosphate - an activated form of vitamin E important for angiogenesis? SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Zingg, Jean-Marc; Meydani, Mohsen; Azzi, Angelo] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711300169 ER PT J AU Zou, YX Liao, ST Liu, F Blumberg, J Chen, CYO AF Zou, Yu-Xiao Liao, Shen-Tai Liu, Fan Blumberg, Jeffrey Chen, C-Y. Oliver TI Phenolics in mulberry leaves protect Hep G2 cells against hyperglycemia-induced oxidative damage SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 21-25, 2012 CL San Diego, CA C1 [Zou, Yu-Xiao; Liao, Shen-Tai; Liu, Fan] Guangdong Acad Agr Sci, Sericulture & Agri Food Res Inst, Guangzhou, Guangdong, Peoples R China. [Blumberg, Jeffrey; Chen, C-Y. Oliver] Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 J9 FASEB J JI Faseb J. PD APR PY 2012 VL 26 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA 032IZ UT WOS:000310711301621 ER PT J AU Kennedy, AH Goldberg, NA Minnis, AM AF Kennedy, Aaron H. Goldberg, Nisse A. Minnis, Andrew M. TI Exobasidium ferrugineae sp nov., associated with hypertrophied flowers of Lyonia ferruginea in the southeastern USA SO MYCOTAXON LA English DT Article DE Basidiomycota; Ericaceae; Exobasidiales; Exobasidiomycetes; plant pathogen ID MIXED MODELS; CHINA; INFERENCE; MRBAYES AB Exobasidium ferrugineae, associated with hypertrophied flowers and less commonly leaves of Lyonia ferruginea (rusty staggerbush), is formally described here as a new species. Morphological and DNA sequence (ITS, nLSU) data are provided. Phylogenetic analyses confirm that it is not conspecific with any species of Exobasidium represented by existing DNA sequence data. A key to North American species of Exobasidium on Lyonia is presented. C1 [Minnis, Andrew M.] US Forest Serv, Ctr Forest Mycol Res, No Res Stn, USDA, Madison, WI 53726 USA. [Kennedy, Aaron H.] USDA APHIS PPQ PHP, Natl Identificat Serv, Beltsville, MD 20705 USA. [Goldberg, Nisse A.] Jacksonville Univ, Dept Biol & Marine Sci, Jacksonville, FL 32211 USA. RP Minnis, AM (reprint author), US Forest Serv, Ctr Forest Mycol Res, No Res Stn, USDA, 1 Gifford Pinochet Dr, Madison, WI 53726 USA. EM amminnis@fs.fed.us NR 28 TC 2 Z9 2 U1 1 U2 2 PU MYCOTAXON LTD PI ITHACA PA PO BOX 264, ITHACA, NY 14851-0264 USA SN 0093-4666 J9 MYCOTAXON JI Mycotaxon PD APR-JUN PY 2012 VL 120 BP 451 EP 460 DI 10.5248/120.451 PG 10 WC Mycology SC Mycology GA 021TQ UT WOS:000309908700053 ER PT J AU Lingafelter, SW Wappes, JE AF Lingafelter, Steven W. Wappes, James E. TI A new species of Trichoxys Chevrolat (Cerambycidae: Cerambycinae: Clytini) from Mexico, with a key to known species SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Insecta; Coleoptera; Cerambycidae; Cerambycinae; Clytini; Trichoxys; new species; key AB The cerambycine genus Trichoxys Chevrolat is diagnosed and a new species from Mexico, T. penrosei Lingafelter & Wappes, is described. Trichoxys ochraetheoides Linsley 1935 is a new synonym of T. hirtellus (Chevrolat 1860). Photos of the elytra of all 15 Trichoxys species are provided, along with a key to species. C1 [Lingafelter, Steven W.] ARS, Systemat Entomol Lab, Inst Plant Sci, USDA,Natl Museum Nat Hist, Washington, DC 20013 USA. [Wappes, James E.] Amer Coleoptera Museum, San Antonio, TX 78255 USA. RP Lingafelter, SW (reprint author), ARS, Systemat Entomol Lab, Inst Plant Sci, USDA,Natl Museum Nat Hist, Washington, DC 20013 USA. EM steve.lingafelter@ars.usda.aov; wappes@earthlink.net NR 7 TC 0 Z9 0 U1 0 U2 1 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2012 VL 88 IS 2 BP 154 EP 162 PG 9 WC Entomology SC Entomology GA 009JD UT WOS:000309021200006 ER PT J AU Seybold, SJ King, JA Harris, DR Nelson, LJ Hamud, SM Chen, YG AF Seybold, Steven J. King, Jennifer A. Harris, Daren R. Nelson, Lori J. Hamud, Shakeeb M. Chen, Yigen TI Diurnal flight response of the walnut twig beetle, Pityophthorus juglandis Blackman (Coleoptera: Scolytidae), to pheromone-baited traps in two northern California walnut habitats SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Aggregation pheromone; bark beetle; California; crepuscular flight activity; diurnal flight behavior; Geosmithia morbida; Juglans hindsii; northern California black walnut; pheromone-trapping; Pityophthorus juglandis; thousand cankers disease; walnut twig beetle ID BARK BEETLES; ATTRACTANTS; GEOSMITHIA; EMERGENCE; PATTERNS; FIELD AB The diurnal flight response of the walnut twig beetle, Pityophthorus juglandis Blackman (Coleoptera: Scolytidae), was assessed during two seasonal periods at two sites in northern California. Males and females flew primarily at dusk in response to aggregation pheromone-baited traps during late June/early July, and the percentage of beetles that flew between sunrise and late afternoon was positively correlated with the temperature at 6 AM of that day. Between late August and early November, the diurnal flight pattern was more varied and generally bimodal, though approx. 42 to 55% of the beetles still flew during the dusk period. During either period of the season, there was very little evidence that any flight occurred during the night. Between late August and late September, diurnal flight of both sexes during two-hour intervals was associated with temperature in a Gaussian manner with a threshold at 17-18 degrees C (62.6-64.4 degrees F) and peak activity at 23-24 degrees C (73.4-75.2 degrees F.). The proportion of females in the catches of P. juglandis that responded to pheromone-baited traps ranged from 0.43 to 0.84 but did not vary throughout the day within a seasonal period. C1 [Seybold, Steven J.; Nelson, Lori J.; Hamud, Shakeeb M.] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA 95616 USA. [King, Jennifer A.; Harris, Daren R.; Chen, Yigen] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. RP Seybold, SJ (reprint author), US Forest Serv, USDA, Pacific SW Res Stn, 720 Olive Dr,Suite D, Davis, CA 95616 USA. EM sjseybold59@gmail.com FU USDA Forest Service, Pacific Southwest Research Station; USDA Forest Service, Washington Office Forest Health Protection; USDA APHIS CPHST [10-CA-11272172-055, 10-JV-11272172-092]; USDA FS PSW Station [10-CA-11272172-055, 10-JV-11272172-092]; UC-Davis Department of Entomology [10-CA-11272172-055, 10-JV-11272172-092] FX We dedicate this manuscript to the late Richard L. Penrose. "Dick" was a good friend and colleague, whose enthusiasm for the detection of invasive bark beetles and woodborers was contagious and stimulating to those around him. From 2004 through 2008, Dick had a big impact on the direction of our research by helping us re-focus from the chemical ecology of native forest insects to using the tools of chemical ecology to detect invading species in urban and pen-urban forest environments. He had a strong, positive influence on the research activities of postdoctoral associates, Jana C. Lee and Deguang Liu, who worked with us on invading elm and pine bark beetles. Dick also assisted with countless identifications of new bark beetles and longhorned beetles. Through his ever perceptive "nose" for new problems, from 2003-2007 Dick did some focused early modern collecting of walnut twig beetles in California. Both from funnel trap catches in his annual CAPS surveys and through rearing of infested walnut, Dick's efforts presaged the current bloom in research on walnut twig beetle, which includes the discoveries reported in this paper. The authors would also like to thank Stacy Hishinuma for assistance with field work and Andrew Graves for assistance with literature retrieval and a helpful review of the manuscript. Funding for this work was provided by the USDA Forest Service, Pacific Southwest Research Station, and by grants from the USDA Forest Service, Washington Office Forest Health Protection and USDA APHIS CPHST administered through research agreements #10-CA-11272172-055 and 10-JV-11272172-092 between the USDA FS PSW Station and the UC-Davis Department of Entomology. We thank John Preece and Howard Garrison (both USDA ARS) for facilitating access to the Wolfskill orchard site. NR 29 TC 8 Z9 8 U1 1 U2 16 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2012 VL 88 IS 2 BP 231 EP 247 PG 17 WC Entomology SC Entomology GA 009JD UT WOS:000309021200013 ER PT J AU Dallara, PL Flint, ML Seybold, SJ AF Dallara, Paul L. Flint, Mary L. Seybold, Steven J. TI An analysis of the larval instars of the walnut twig beetle, Pityophthorus juglandis Blackman (Coleoptera: Scolytidae), in northern California black walnut, Juglans hindsii, and a new host record for Hylocurus hirtellus SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Bark beetle; California; California hardwood bark beetle; Coleoptera; Geosmithia morbida; head capsule; Hylocurus hirtellus; instar; Juglans hindsii; life history; northern California black walnut; Pityophthorus juglandis; Scolytidae; shrub bark beetle; thousand cankers disease; walnut twig beetle ID HEAD-CAPSULE WIDTHS; ELM BARK BEETLE; LIFE-HISTORY; FREQUENCY-DISTRIBUTION; LECONTE COLEOPTERA; NAVEL ORANGEWORM; LEPIDOPTERA; PYRALIDAE; BIOLOGY; PINE AB By measuring and analyzing larval head capsule widths, we determined that a northern California population of the walnut twig beetle, Pityophthorus juglandis Blackman (Coleoptera: Scolytidae), has three larval instars. We also developed rules to classify P. juglandis larval instars. Overlap in the ranges of widths among consecutive instars was addressed by fitting a distribution model to the data and determining limits for head capsule width classes and probabilities of instar misclassification. Growth ratios for laboratory and field samples of P. juglandis were consistent with the Brooks-Dyar rule and with results in the literature for other bark and ambrosia beetle species. As a consequence of branch dissection to retrieve larvae of P. juglandis, we established that northern California black walnut, Juglans hindsii (Jeps.) Jeps. ex R.E. Sm., is a host for the xylophagous "California hardwood bark beetle," Hylocurus hirtellus (LeConte) (Coleoptera: Scolytidae), and that H. hirtellus is associated with P. juglandis beneath the bark of small diameter branches. It appears that in northern California, univoltine H. hirtellus is active in flight from early March through early May, with the majority of the adults dispersing in April. The females are the colonizing sex. C1 [Dallara, Paul L.; Flint, Mary L.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA 95616 USA. RP Dallara, PL (reprint author), Univ Calif Davis, Dept Entomol, 1 Shields Ave, Davis, CA 95616 USA. EM sjseybold59@gmail.com FU USDA Forest Service, Pacific Southwest Research Station; USDA Forest Service, Washington Office Forest Health Protection; USDA APHIS CPHST [10-CA-11272172-055, 10-JV-11272172-092]; USDA FS PSW Station [10-CA-11272172-055, 10-JV-11272172-092]; UC Davis Department of Entomology [10-CA-11272172-055, 10-JV-11272172-092] FX We dedicate this manuscript to the late Richard L. Penrose. "Dick" was a good friend and colleague, whose enthusiasm for the detection of invasive bark beetles and woodborers was contagious and stimulating to those around him. From 2004 through 2008, Dick had a big impact on the direction of research in our labs by helping us shift focus from the chemical ecology of native forest insects to using the tools of chemical ecology to detect invading species in urban and pen-urban forest environments. He had a strong, positive influence on the research activities of postdoctoral associates, Jana C. Lee and Deguang Liu, who worked with us on invading elm and pine bark beetles. Dick also assisted with countless identifications of new bark beetles and longhorned beetles. Due to his ever perceptive "nose" for new problems, from 2003-2007 Dick did some focused early "modern" collecting of walnut twig beetles in California. Both from funnel trap catches in his annual CAPS surveys and through rearing of infested walnut, Dick's efforts presaged the current bloom in research on walnut twig beetle, which includes the discoveries reported in this paper. We thank A.D. Graves (USDA Forest Service, Albuquerque, NM) for assistance with preparing the Yolo Co. WTB samples, and S.M. Hishinuma (UC Davis, Department of Entomology, Davis, California) and R.C. Venette (USDA Forest Service, St. Paul, Minnesota) for critical reviews of the manuscript. S.M. Hishinuma and J.A. Tillman assisted with the graphics for this paper. Funding for this work was provided by the USDA Forest Service, Pacific Southwest Research Station, and by grants from the USDA Forest Service, Washington Office Forest Health Protection and USDA APHIS CPHST administered through cooperative agreements #10-CA-11272172-055 and 10-JV-11272172-092 between the USDA FS PSW Station and the UC Davis Department of Entomology. The East Bay Regional Parks District allowed the use of park properties for collection and Field trials. We thank J. Honda for facilitating the loan of the specimens of H. hirtellus fron San Jose State University and D. E. Bright for determination of H. hirtellus. NR 69 TC 1 Z9 3 U1 2 U2 20 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2012 VL 88 IS 2 BP 248 EP 266 PG 19 WC Entomology SC Entomology GA 009JD UT WOS:000309021200014 ER PT J AU Haavik, LJ Coleman, TW Chen, YG Jones, MI Venette, RC Flint, ML Seybold, SJ AF Haavik, Laurel J. Coleman, Tom W. Chen, Yigen Jones, Michael I. Venette, Robert C. Flint, Mary L. Seybold, Steven J. TI First occurrence of the goldspotted oak borer parasitoid, Calosota elongata (Hymenoptera: Eupelmidae), in California SO PAN-PACIFIC ENTOMOLOGIST LA English DT Editorial Material ID SCHAEFFER COLEOPTERA BUPRESTIDAE; AGRILUS-AUROGUTTATUS; WOODLANDS C1 [Haavik, Laurel J.; Chen, Yigen; Jones, Michael I.; Flint, Mary L.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Coleman, Tom W.] US Forest Serv, USDA, San Bernardino, CA 92408 USA. [Venette, Robert C.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific SW Res Stn, St Paul, MN 55108 USA. RP Haavik, LJ (reprint author), Univ Calif Davis, Dept Entomol, 1 Shields Ave, Davis, CA 95616 USA. EM sjseybold59@gmail.com OI Haavik, Laurel/0000-0002-7749-9095 NR 7 TC 3 Z9 3 U1 1 U2 19 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2012 VL 88 IS 2 BP 274 EP 276 PG 3 WC Entomology SC Entomology GA 009JD UT WOS:000309021200016 ER PT J AU Seybold, SJ Coleman, TW Dallara, PL Dart, NL Graves, AD Pederson, LA Spichiger, SE AF Seybold, Steven J. Coleman, Tom W. Dallara, Paul L. Dart, Norman L. Graves, Andrew D. Pederson, Lee A. Spichiger, Sven-Erik TI Recent collecting reveals new state records and geographic extremes in the distribution of the walnut twig beetle, Pityophthorus juglandis Blackman (Coleoptera: Scolytidae), in the United States SO PAN-PACIFIC ENTOMOLOGIST LA English DT Editorial Material C1 [Seybold, Steven J.] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA 95616 USA. [Coleman, Tom W.] US Forest Serv, USDA, San Bernardino, CA 92408 USA. [Dallara, Paul L.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. [Dart, Norman L.] Virginia Dept Agr & Consumer Serv, Off Plant Ind Serv, Richmond, VA 23219 USA. [Graves, Andrew D.] US Forest Serv, USDA, Albuquerque, NM 87102 USA. [Pederson, Lee A.] US Forest Serv, USDA, Coeur Dalene, ID 83815 USA. [Spichiger, Sven-Erik] Penn Dept Agr, Harrisburg, PA 17110 USA. RP Seybold, SJ (reprint author), US Forest Serv, USDA, Pacific SW Res Stn, 720 Olive Dr,Suite D, Davis, CA 95616 USA. EM sjseybold59@gmail.com NR 5 TC 12 Z9 13 U1 1 U2 8 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2012 VL 88 IS 2 BP 277 EP 280 PG 4 WC Entomology SC Entomology GA 009JD UT WOS:000309021200017 ER PT J AU Daniel, HM Redhead, SA Schnurer, J Naumov, GI Kurtzman, CP AF Daniel, Heide-Marie Redhead, Scott A. Schnurer, Johan Naumov, Gennadi I. Kurtzman, Cletus P. TI (2049-2050) Proposals to conserve the name Wickerhamomyces against Hansenula and to reject the name Saccharomyces sphaericus (Ascomycota: Saccharomycotina) SO TAXON LA English DT Editorial Material C1 [Daniel, Heide-Marie] Catholic Univ Louvain, Mycotheque Univ Catholic Louvain, Earth & Life Inst, B-1348 Louvain, Belgium. [Redhead, Scott A.] Agr & Agri Food Canada, Natl Mycol Herbarium, Ottawa, ON K1A 0C6, Canada. [Schnurer, Johan] Swedish Univ Agr Sci SLU, Dept Microbiol, S-75007 Uppsala, Sweden. [Naumov, Gennadi I.] State Inst Genet & Select Ind Microorganisms, Moscow 117545, Russia. [Kurtzman, Cletus P.] USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. RP Daniel, HM (reprint author), Catholic Univ Louvain, Mycotheque Univ Catholic Louvain, Earth & Life Inst, Croix Sud 2,Bte L7-05-06, B-1348 Louvain, Belgium. EM heide-marie.daniel@uclouvain.be NR 0 TC 0 Z9 0 U1 1 U2 8 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 J9 TAXON JI Taxon PD APR PY 2012 VL 61 IS 2 BP 459 EP 461 PG 3 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 010GN UT WOS:000309082900001 ER PT J AU Seifert, KA Frisvad, JC Houbraken, J Llimona, X Peterson, SW Samson, RA Visagie, CM AF Seifert, Keith A. Frisvad, Jens C. Houbraken, Jos Llimona, Xavier Peterson, Stephen W. Samson, Robert A. Visagie, Cobus M. TI (2051) Proposal to conserve the name Talaromyces over Lasioderma (Ascomycota) SO TAXON LA English DT Editorial Material C1 [Seifert, Keith A.] Agr & Agri Food Canada, Biodivers Mycol Eastern Cereal & Oilseed Res Ctr, Ottawa, ON K1A 0C6, Canada. [Frisvad, Jens C.] Tech Univ Denmark, Dept Syst Biol, DK-2800 Lyngby, Denmark. [Houbraken, Jos; Samson, Robert A.] CBS KNAW Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands. [Llimona, Xavier] Univ Barcelona, Dept Biol Vegetal Bot, E-08028 Barcelona, Spain. [Peterson, Stephen W.] USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. [Visagie, Cobus M.] Univ Stellenbosch, Dept Microbiol, ZA-7600 Stellenbosch, South Africa. RP Seifert, KA (reprint author), Agr & Agri Food Canada, Biodivers Mycol Eastern Cereal & Oilseed Res Ctr, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada. EM keith.seifert@agr.gc.ca NR 0 TC 2 Z9 2 U1 0 U2 6 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 J9 TAXON JI Taxon PD APR PY 2012 VL 61 IS 2 BP 461 EP 462 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 010GN UT WOS:000309082900002 ER PT J AU Meng, L Zhu, XY Kirkbride, JH AF Meng, Lei Zhu, Xiang-Yun Kirkbride, Joseph H., Jr. TI (2059) Proposal to conserve the name Glycyrrhiza inflata against G. paucifoliolata (Leguminosae) SO TAXON LA English DT Editorial Material C1 [Meng, Lei; Zhu, Xiang-Yun] Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China. [Meng, Lei] China Agr Univ, Coll Biol, Beijing 100193, Peoples R China. [Kirkbride, Joseph H., Jr.] US Natl Arboretum, USDA ARS, Washington, DC 20002 USA. RP Zhu, XY (reprint author), Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China. EM xiangyunzhu@ibcas.ac.cn NR 0 TC 0 Z9 0 U1 0 U2 2 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 J9 TAXON JI Taxon PD APR PY 2012 VL 61 IS 2 BP 471 EP 471 PG 1 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 010GN UT WOS:000309082900010 ER PT J AU Kirkbride, JH Wiersema, JH Delprete, PG AF Kirkbride, Joseph H., Jr. Wiersema, John H. Delprete, Piero G. TI (2063-2065) Proposals to reject the names Spermacoce strigosa and S. hyssopifolia Sm. and conserve the name S. hyssopifolia Willd. ex Roem. & Schult. (Rubiaceae) SO TAXON LA English DT Editorial Material C1 [Kirkbride, Joseph H., Jr.] ARS, USDA, US Natl Arboretum, Washington, DC 20002 USA. [Wiersema, John H.] ARS, USDA, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Delprete, Piero G.] IRD UMR AMAP, Herbier Guyane, Cayenne 97323, French Guiana. RP Kirkbride, JH (reprint author), ARS, USDA, US Natl Arboretum, 3501 New York Ave NE, Washington, DC 20002 USA. EM joseph.kirkbride@ars.usda.gov OI Delprete, Piero/0000-0001-5844-3945 NR 0 TC 1 Z9 1 U1 0 U2 1 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 J9 TAXON JI Taxon PD APR PY 2012 VL 61 IS 2 BP 475 EP 476 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 010GN UT WOS:000309082900014 ER PT J AU Lebed, L Qi, J Heilman, P AF Lebed, L. Qi, J. Heilman, P. TI An ecological assessment of pasturelands in the Balkhash area of Kazakhstan with remote sensing and models SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE pasturelands; ecological capacity; remote sensing; Kazakhstan climate ID MANAGEMENT AB The 187 million hectares of pasturelands in Kazakhstan play a key role in the nation's economy, as livestock production accounted for 54% of total agricultural production in 2010. However, more than half of these lands have been degraded as a result of unregulated grazing practices. Therefore, effective long term ecological monitoring of pasturelands in Kazakhstan is imperative to ensure sustainable pastureland management. As a case study in this research, we demonstrated how the ecological conditions could be assessed with remote sensing technologies and pastureland models. The example focuses on the southern Balkhash area with study sites on a foothill plain with Artemisia-ephemeral plants and a sandy plain with psammophilic vegetation in the Turan Desert. The assessment was based on remotely sensed imagery and meteorological data, a geobotanical archive and periodic ground sampling. The Pasture agrometeorological model was used to calculate biological, ecological and economic indicators to assess pastureland condition. The results showed that field surveys, meteorological observations, remote sensing and ecological models, such as Pasture, could be combined to effectively assess the ecological conditions of pasturelands and provide information about forage production that is critically important for balancing grazing and ecological conservation. C1 [Lebed, L.] Kazakh Res Inst Ecol & Climate, Alma Ata, Kazakhstan. [Qi, J.] Michigan State Univ, Ctr Global Change & Earth Observat, E Lansing, MI 48824 USA. [Heilman, P.] USDA ARS, SW Watershed Res Ctr, Tucson, AZ USA. RP Lebed, L (reprint author), Kazakh Res Inst Ecol & Climate, Alma Ata, Kazakhstan. EM llebed@inbox.ru; qi@msu.edu; phil.heilman@ars.usda.gov FU International Science and Technology Center (ISTC) [K-1396p]; NASA's support on pastureland decision project [NNX10AB65G] FX We thank three anonymous reviewers for comments that substantially improved this paper. This research was supported by the International Science and Technology Center (ISTC), Project Agreement #K-1396p and NASA's support on pastureland decision project NNX10AB65G. NR 16 TC 5 Z9 6 U1 0 U2 15 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD APR-JUN PY 2012 VL 7 IS 2 AR 025203 DI 10.1088/1748-9326/7/2/025203 PG 8 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 989VZ UT WOS:000307590300033 ER PT J AU Liu, B Zhang, JW Liu, LS Hotchkiss, AT AF Liu, Bo Zhang, Jinwen Liu, Linshu Hotchkiss, Arland T. TI Utilization of Pectin Extracted Sugar Beet Pulp for Composite Application SO JOURNAL OF BIOBASED MATERIALS AND BIOENERGY LA English DT Article DE Composite; Pectin; Sugar Beet Pulp ID POLY(LACTIC ACID); SOY PROTEIN; CELLULOSE MICROFIBRILS; MECHANICAL-BEHAVIOR; GREEN COMPOSITES; BLENDS; MORPHOLOGY; EXTRUSION; GLYCEROL; STARCH AB Sugar beet pulp (SBP) is the residue left after beet sugar extraction. SBP contains similar to 25% pectin and is an important resource for pectin. However, sugar beet pectin does not have good gel-forming properties and complete extraction of pectin is not typically performed due to the low quality of the galacturonic acid-containing polysaccharide remaining following commercial pectin extraction. This study was to find out what level of residual pectin is needed for making SBP composites and the effects of pectin content on the properties of the resulting composites. In this work, the pectin extracted SBP (PE-SBP) was recombined with pectin to prepare SBP composites. The compounding of pectin and PE-SBP was conducted using a twin-screw extruder, and water and glycerol were used as plasticizers. Pectin was plasticized during extrusion and behaved like a binder or matrix for the pectin/PE-SBP compounds, depending on pectin content. Mechanical and thermal properties of the compounds were studied and the results showed that utilization of the PE-SBP as a resource for plastic materials is possible. C1 [Liu, Bo; Zhang, Jinwen] Washington State Univ, Mat Sci & Engn Program, Pullman, WA 99164 USA. [Liu, Bo; Zhang, Jinwen] Washington State Univ, Composite Mat & Engn Ctr, Pullman, WA 99164 USA. [Liu, Linshu; Hotchkiss, Arland T.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Zhang, JW (reprint author), Washington State Univ, Mat Sci & Engn Program, Pullman, WA 99164 USA. EM jwzhang@wsu.edu; linshu.liu@ars.usda.gov RI Liu, Bo/A-9425-2010; Zhang, Jinwen/G-8674-2014 OI Liu, Bo/0000-0003-4561-1051; Zhang, Jinwen/0000-0001-8828-114X FU Agricultural Research Service of the United States Department of Agriculture [58-1935-9-965] FX The authors are grateful for financial support from the Agricultural Research Service of the United States Department of Agriculture, specific cooperative project #58-1935-9-965. NR 33 TC 3 Z9 3 U1 2 U2 27 PU AMER SCIENTIFIC PUBLISHERS PI VALENCIA PA 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA SN 1556-6560 J9 J BIOBASED MATER BIO JI J. Biobased Mater. Bioenergy PD APR PY 2012 VL 6 IS 2 BP 185 EP 192 DI 10.1166/jbmb.2012.1206 PG 8 WC Chemistry, Applied; Energy & Fuels; Materials Science, Biomaterials SC Chemistry; Energy & Fuels; Materials Science GA 998QK UT WOS:000308255300008 ER PT J AU Elliott, KJ Vose, JM AF Elliott, Katherine J. Vose, James M. TI Age and distribution of an evergreen clonal shrub in the Coweeta Basin: Rhododendron maximum L. SO JOURNAL OF THE TORREY BOTANICAL SOCIETY LA English DT Article DE Dendrochronology; riparian areas; Southern Appalachians; streams ID MOUNTAINS NATIONAL-PARK; CANOPY TREE SEEDLINGS; SOUTHERN APPALACHIANS; LONG-TERM; CARBON GAIN; LIGHT ENVIRONMENT; UNDERSTORY SHRUBS; KALMIA-LATIFOLIA; TSUGA-CANADENSIS; LEAF MOVEMENTS AB ELLIOTT, K. J. AND J. M. VOSE (Coweeta Hydrologic Laboratory, Center for Forest Watershed Science, SRS-4353, Southern Research Station, USDA Forest Service, Otto, NC 28763). Age and distribution of an evergreen clonal shrub in the Coweeta Basin: Rhododendron maximum L. J. Torrey Bot. Soc. 139: 149-166. 2012. Rhododendron maximum L. is an evergreen, clonal shrub that forms a dominant sub-canopy layer and is a key species in southern Appalachian forests. We investigated the age and distribution of R. maximum across the Coweeta Basin, a 1626 ha watershed in western North Carolina. We selected 16 perennial, second-order streams and used a Global Positioning System to establish site boundaries and map the coverage of R. maximum across the hillslopes from stream to ridge. In each site, three transects from stream edge to the ridge were used to measure diameters of overstory trees (>= 2.5 cm dbh), tree saplings (< 2.5 cm dbh) and shrubs including R. maximum stems. Along each transect, we cut cross-sections of R. maximum ramets and extracted increment cores from nearest neighbor trees to determine ages. The 16 sites ranged in size from 0.3 to 1.9 ha depending on the distance from stream to ridge. Rhododendron maximum cover ranged from 25 to 100% and ages ranged from 6 to 120 years. Rhododendron maximum establishment year showed a skewed unimodal distribution with the peak establishment occurring between 1928 and 1940. Although the R. maximum age and distance-from-stream relationship was statistically significant, the relationship was not meaningful as distance-from-stream only explained 2.6% of the variation in R. maximum age (r(2) = 0.026, P = 0.0003, n = 487). Distance from stream only explained 4.2% of the variation in overstory tree age (r(2) = 0.042, P = 0.0015, n=237). It appears that R. maximum has not expanded upslope over the last 100 years; rather the ranges in sizes and ages suggest that ramets are recruiting under established R. maximum canopies particularly in the wetter, near stream locations. C1 [Elliott, Katherine J.; Vose, James M.] US Forest Serv, Coweeta Hydrol Lab, Ctr Forest Watershed Sci, So Res Stn,USDA, Otto, NC 28763 USA. RP Elliott, KJ (reprint author), US Forest Serv, Coweeta Hydrol Lab, Ctr Forest Watershed Sci, So Res Stn,USDA, SRS 4353, Otto, NC 28763 USA. EM kelliott@fs.fed.us FU USDA Forest Service, Southern Research Station; National Science Foundation [DEB0218001] FX This study was primarily supported by the USDA Forest Service, Southern Research Station and partially supported by National Science Foundation grant DEB0218001 to the Coweeta LTER program at the University of Georgia. We are grateful to Drs. Steven Brantley and Ryan McEwan for providing helpful comments on this manuscript. We also acknowledge the valuable contributions of Patsy Clinton who collected vegetation data and provided the technical expertise on dendrochronology and GIS, and other Forest Service staff who provided field assistance. NR 84 TC 3 Z9 3 U1 1 U2 19 PU TORREY BOTANICAL SOC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 1095-5674 J9 J TORREY BOT SOC JI J. Torrey Bot. Soc. PD APR-JUN PY 2012 VL 139 IS 2 BP 149 EP 166 PG 18 WC Plant Sciences SC Plant Sciences GA 985JD UT WOS:000307259500005 ER PT J AU Sutton, BD Steck, GJ Norrbom, AL AF Sutton, Bruce D. Steck, Gary J. Norrbom, Allen L. TI New species of Gymnocarena (Diptera: Tephritidae) from eastern North America and Guatemala, and the redescription of G. mississippiensis SO CANADIAN ENTOMOLOGIST LA English DT Article AB We describe three new species of fruit flies (Tephritidae: Tephritinae) (Gymnocarena defoei sp. nov. and Gymnocarena norrbomi sp. nov., from eastern North America and Gymnocarena monzoni sp. nov. from Guatemala) and redescribe Gymnocarena mississippiensis Norrbom. Gymnocarena monzoni is the first Gymnocarena species to be recorded from Guatemala. This brings the total number of named species in this genus to 19. New larval host plant (Asteraceae) records for Gymnocarena include Verbesina helianthoides Michx. for G. mississippiensis and G. norrbomi, Verbesina alternifolia (L.) Britton ex Kearney for G. norrbomi, and Viguiera cordata (Hook. and Arn.) D'Arcy for G. monzoni. The latter represents the first record for Gymnocarena in Viguiera Kunth. Gymnocarena larvae were also recorded from Verbesina virginica L. but not identified to species. A revised key to the known species of Gymnocarena and additional information on larval host plants and biology are provided. C1 [Sutton, Bruce D.] Florida Dept Agr & Consumer Serv, Bur Entomol Nematol & Plant Pathol, Florida State Collect Arthropods & Adv Diagnost L, Gainesville, FL 32614 USA. [Steck, Gary J.] Florida Dept Agr & Consumer Serv, Bur Entomol Nematol & Plant Pathol, Florida State Collect Arthropods & Entomol Sect, Gainesville, FL 32614 USA. [Norrbom, Allen L.] Natl Museum Nat Hist, USDA, ARS, Systemat Entomol Lab, Washington, DC 20013 USA. RP Sutton, BD (reprint author), Florida Dept Agr & Consumer Serv, Bur Entomol Nematol & Plant Pathol, Florida State Collect Arthropods & Adv Diagnost L, POB 147100, Gainesville, FL 32614 USA. EM suttonb@doacs.state.fl.us FU DLIA; Laboratorio de Entomologia Sistematica and Coleccion de Artropodos of the Universidad del Valle de Guatemala (UVG) FX We thank the U. S. National Park Service, GSMNP, biological staff at Twin Creeks for facilitating our work in the park. Special thanks go to Keith Langdon, Inventory and Monitoring Coordinator for GSMNP, the late Don Defoe, Adriean Mayer, Becky Nichols, and Paul Super. Specimens were collected under NPS permit GRSM-00-103. Janet Rock (GSMNP) kindly identified or confirmed identifications of host plants. We also thank Ms. Jeanie Hilton (formerly of Discover Life in America [DLIA]) and others at DLIA for providing accommodations to support the fieldwork for the "Tephritidae of GSMNP'' project as part of the All Taxon Biological Inventory of GSMNP. Funding for research in GSMNP was provided by DLIA. We thank Judy Hallisey (formerly Environmental Stewardship Department Manager, Land Between the Lakes, U.S. Forest Service) for facilitating our collecting at Land Between the Lakes National Recreation Area, Kentucky. We also thank the Comision Nacional de Areas Protegidas (CONAP) for permits for Guatemala, the Fundacion Defensores de la Naturaleza Guatemala for permission to collect in the Sierra de las Minas Biosphere Reserve, the personnel of the San Lorenzo Marble Quarry, with special thanks to Senor Perdomo, for permitting access to the property, the Laboratorio de Entomologia Sistematica and Coleccion de Artropodos of the Universidad del Valle de Guatemala (UVG) for support, and Jose Monzon Sierra (UVG) for his invaluable assistance without which our fieldwork in his country would have been far more difficult. Our very special thanks goes to our good friend and colleague Jack Schuster (UVG) for his dedication and contributions to our understanding of the biodiversity and biogeography of Guatemala and for his enabling of our work in that country. We are indebted to Harold Robinson of the Smithsonian Institution, Washington, District of Columbia, for his identification of Asteraceae from Guatemala. We thank Bill Grogan (FSCA), Wayne Mathis (Smithsonian Institution), Norm Woodley and A. Konstantinov (Systematic Entomology Laboratory, USDA, ARS), and two anonymous reviewers for their comments on the manuscript. This is contribution number 1194 of the Bureau of Entomology, Nematology, and Plant Pathology, Division of Plant Industry, Florida Department of Agriculture and Consumer Services, Gainesville, Florida, United States of America. NR 3 TC 0 Z9 0 U1 1 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD APR PY 2012 VL 144 IS 2 BP 248 EP 265 DI 10.4039/tce.2012.25 PG 18 WC Entomology SC Entomology GA 984ZJ UT WOS:000307232200005 ER PT J AU Runyon, JB AF Runyon, Justin B. TI The Nearctic species of Telmaturgus (Diptera: Dolichopodidae) SO CANADIAN ENTOMOLOGIST LA English DT Article AB The sympycnine genus Telmaturgus Mik is reviewed for the Nearctic region, and includes three species: Telmaturgus parvus (Van Duzee), Telmaturgus robinsoni sp. nov., and Telmaturgus vockerothi sp. nov. Species of Telmaturgus can be recognized by the reduced number of dorsocentral setae (three pairs in Nearctic species) and the projecting lower face of females. Examination of the holotype of T. parvus reveals that the widespread species previously thought to be T. parvus is T. robinsoni. A key to species and comments on the taxonomic history and ecology of the genus are provided. C1 [Runyon, Justin B.] USDA Forest Serv, Rocky Mt Res Stn, Bozeman, MT 59717 USA. [Runyon, Justin B.] Montana State Univ, Marsh Lab, Bozeman, MT 59717 USA. RP Runyon, JB (reprint author), USDA Forest Serv, Rocky Mt Res Stn, 1648 S 7th Ave, Bozeman, MT 59717 USA. EM jrunyon@montana.edu OI Runyon, Justin B./0000-0002-0271-0511 NR 13 TC 0 Z9 0 U1 0 U2 4 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X EI 1918-3240 J9 CAN ENTOMOL JI Can. Entomol. PD APR PY 2012 VL 144 IS 2 BP 337 EP 347 DI 10.4039/tce.2012.30 PG 11 WC Entomology SC Entomology GA 984ZJ UT WOS:000307232200010 ER PT J AU Kamil, A Chen, CYO AF Kamil, Alison Chen, C-Y. Oliver TI Nuts for Diabetes Prevention and Management SO JOURNAL OF FOOD AND DRUG ANALYSIS LA English DT Article; Proceedings Paper CT 5th International Conference on Food Factors (ICoFF) CY NOV 20-23, 2011 CL Taipei, TAIWAN DE Nuts; diabetes; glycemia; body weight; lipids; endothelial function; hypertension; antioxidant; anti-inflammatory ID POSTPRANDIAL GLYCEMIA; ENDOTHELIAL FUNCTION; METABOLIC SYNDROME; CROSSOVER TRIAL; LIFE-STYLE; LONG-TERM; CONSUMPTION; RISK; DIET; MELLITUS AB Type 2 diabetes mellitus is an important preventable disease and a growing public health problem. Epidemiologic and clinical studies suggest that healthy eating, physical activity, and BW control are the main driving forces to reduce diabetes risk. Owing to their low available carbohydrate content, favorable fat and protein profile as well as phytonutrient content, nut consumption has been associated with a reduced risk of development and management of diabetes. Nuts, by virtue of its cardioprotective actions, have also been shown to reduce biomarkers of risk factors for diabetic complications. Although more studies are warranted, the emerging picture is that nut consumption beneficially influences diabetes risk and management beyond blood glucose control. C1 [Kamil, Alison; Chen, C-Y. Oliver] Tufts Univ, Antioxidants Res Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Chen, CYO (reprint author), Tufts Univ, Antioxidants Res Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM Oliver.Chen@tufts.edu NR 32 TC 0 Z9 0 U1 1 U2 6 PU BUREAU FOOD DRUG ANALYSIS PI TAIPEI PA 161-2 KUNYANG STREET, NANGANG, TAIPEI, 00000, TAIWAN SN 1021-9498 J9 J FOOD DRUG ANAL JI J. Food Drug Anal. PD APR PY 2012 VL 20 SU 1 BP 323 EP 327 PG 5 WC Food Science & Technology; Pharmacology & Pharmacy SC Food Science & Technology; Pharmacology & Pharmacy GA 969AG UT WOS:000306027500035 ER PT J AU Ulyshen, MD Hanula, JL Blinn, RL Kritsky, G AF Ulyshen, Michael D. Hanula, James L. Blinn, Robert L. Kritsky, Gene TI Saproxylic Hemiptera Habitat Associations SO SOUTHEASTERN NATURALIST LA English DT Article ID SOUTHEASTERN UNITED-STATES; HETEROPTERA; ARADIDAE; BEETLES; USA AB Understanding the habitat requirements of organisms associated with dead wood is important in order to conserve them in managed forests. Unfortunately, many of the less diverse saproxylic taxa, including Hemiptera, remain largely unstudied. An effort to rear insects from dead wood taken from two forest types (an upland pine-dominated and a bottomland mixed hardwood), three tree species (Liquidambar styraciflua [Sweetgum], Pinus taeda [Loblolly Pine], and Quercus nigra [Water Oak]), and two wood postures (standing snags and fallen logs) in South Carolina produced 435 Hemiptera belonging to eight families and 14 species. The most common (>25 individuals) species were Lyctocoris stalii, Systelloderes inusitatus, Lasiochilus fusculus, Mezira granulata, Calisius contubernalis (a new state record), and Catonia sp. Lyctocoris stalii and Systelloderes inusitatus were almost exclusively captured in the upland and bottomland forest, respectively. Systelloderes inusitatus and Mezira granulata were recovered only from logs. Catonia sp. only emerged from P. taeda logs. Among the less common species, all but two of the 21 specimens of Peritropis saldaeformis were collected from snags. Similarly, all four specimens of Calliodis temnostethoides collected emerged from the crowns of snags. These findings strongly indicate that saproxylic Hemiptera are unevenly distributed among forests, tree species, and wood postures in the southeastern United States. A wide variety of dead wood is clearly necessary to maintain this fauna. C1 [Ulyshen, Michael D.] US Forest Serv, USDA, So Res Stn, Starkville, MS 39759 USA. [Hanula, James L.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. [Blinn, Robert L.] N Carolina State Univ, Dept Entomol, Raleigh, NC 27607 USA. [Kritsky, Gene] Coll Mt St Joseph, Dept Biol, Cincinnati, OH 45233 USA. RP Ulyshen, MD (reprint author), US Forest Serv, USDA, So Res Stn, Starkville, MS 39759 USA. EM mulyshen@fs.fed.us NR 20 TC 2 Z9 2 U1 1 U2 5 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 J9 SOUTHEAST NAT JI Southeast. Nat. PD APR PY 2012 VL 11 IS 1 BP 135 EP 140 DI 10.1656/058.011.0113 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 982AB UT WOS:000307013000014 ER PT J AU Luna-Nevarez, P Rincon, G Medrano, JF Riley, DG Chase, CC Coleman, SW DeAtley, KL Islas-Trejo, A Silver, GA Thomas, MG AF Luna-Nevarez, Pablo Rincon, Gonzalo Medrano, Juan F. Riley, David G. Chase, Chad C., Jr. Coleman, Sam W. DeAtley, Kasey L. Islas-Trejo, A. Silver, Gail A. Thomas, Milton G. TI Identification of one polymorphism from the PAPP-A2 gene associated to fertility in Romosinuano beef heifers raised under a subtropical environment SO REVISTA MEXICANA DE CIENCIAS PECUARIAS LA English DT Article DE PAPPA2; Reproductive performance; Romosinuano; SNP ID QUANTITATIVE TRAIT LOCI; GROWTH-HORMONE RECEPTOR; PLASMA PROTEIN-A2; PRODUCTIVE LIFE; CANDIDATE GENE; CATTLE; BRAHMAN; PERFORMANCE; EXPRESSION; PATHWAY AB The objective was to identify single nucleotide polymorphism (SNP) associated to fertility in cows raised under a subtropical environment. Re-sequencing of nine genes associated to GH-IGF endocrine pathway, which are located in bovine chromosomes 5, 16 and 20, identified 73 SNP useful for associative genetic studies; however, only seven resulted as polymorphic and unique to the Romosinuano breed. Then, DNA samples were extracted from 129 beef heifers and used to determine genotypes corresponding to each SNP. Mixed model analysis identified one SNP from the PAPP-A2 gene (C/T, rs110490898) as predictor (P < 0.05) of reproductive performance. The allele T was the most favorable allele (P < 0.05), because it was associated with lower age at first calving (-37.1 +/- 14.4 d) and age at second calving (-65.43 +/- 30.8 d). In the contrast analysis, the linear term resulted as significant and quadratic term as non significant, which suggested an additive effect of the alleles. These results provide evidence to support the PAPP-A2 gene as a candidate gene associated to reproductive performance in heifers and first-calf cows from the Romosinuano breed, raised under a subtropical environment. C1 [Luna-Nevarez, Pablo] Inst Tecnol Sonora, Dept Ciencias Agron & Vet, Obregon 85000, Sonora, Mexico. [Rincon, Gonzalo; Medrano, Juan F.; Islas-Trejo, A.] Univ Calif Davis, Dept Ciencia Anim, Davis, CA 95616 USA. [Riley, David G.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [Chase, Chad C., Jr.; Coleman, Sam W.] Estn Invest Agr Subtrop, USDA ARS, Brooksville, FL USA. [DeAtley, Kasey L.; Silver, Gail A.; Thomas, Milton G.] Univ Estatal Nuevo Mexico, Dept Ciencias Anim & Pecuaria, Mexico City, DF, Mexico. RP Luna-Nevarez, P (reprint author), Inst Tecnol Sonora, Dept Ciencias Agron & Vet, Obregon 85000, Sonora, Mexico. EM pluna@itson.mx NR 41 TC 2 Z9 2 U1 0 U2 2 PU INIFAP-CENID PARASITOLOGIA VETERINARIA PI JIUTEPEC C P PA CARRETERA FEDERAL CUERNAVACA-CUAUTIA NO 8534, COL PROGRESSO, JIUTEPEC C P, 06300 D F, MEXICO SN 2007-1124 J9 REV MEX CIENC PECU JI Rev. Mex. Cienc. Pecu. PD APR-JUN PY 2012 VL 3 IS 2 BP 185 EP 200 PG 16 WC Agriculture, Dairy & Animal Science SC Agriculture GA 977EX UT WOS:000306640300004 ER PT J AU Vieira, LD Soares, TL Rossi, ML Alves, AAC dos Santos, FDR Souzas, FVD AF Vieira, Livia de Jesus Soares, Taliane Leila Rossi, Monica Lanzoni Cunha Alves, Alfredo Augusto Ribeiro dos Santos, Francisco de Assis Duarte Souzas, Fernanda Vidigal TI Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae) SO ACTA BOTANICA BRASILICA LA English DT Article DE Manihot esculenta Crantz; pre-breeding; genetic resources ID CASSAVA AB (Viability, production and morphology of pollen grains for different species in the genus Manihot (Euphorbiaceae)). The objective of this work was to characterize the viability, production and morphology of pollen for different species in the genus Manihot. Floral buds from Manihot accessions were collected from two germplasm banks at Embrapa Cassava & Fruits. The viability of the pollen was assessed via colorimetric, in vitro and in vivo assays. The diameter of the pollen grains was determined by measuring the transversal length of the grain. The experimental design was entirely randomized. Studies on pollen ultrastructure were performed via scanning electron microscopy. Pollen viability was high in the colorimetric tests and intermediate in vivo tests; there was no germination in the in vitro tests. The average production for all accessions was 1,253 pollen grains per floral bud. The size of the pollen grains varied from 132 to 163 pm in the wild accessions, and 129 to 146 pm in the cultivated accessions. The pollen grains for all accessions were very large, apolar, spherical as well as inaperturate, with an exine ornamented with pila organized in a Croton pattern. The wild accessions, in general, produced more and larger pollen grains compared with the cultivated accessions. C1 [Vieira, Livia de Jesus; Soares, Taliane Leila] Univ Fed Reconcavo Bahia, Cruz Das Almas, BA, Brazil. [Rossi, Monica Lanzoni] Univ Sao Paulo, Ctr Energia Nucl Agr, Piracicaba, SP, Brazil. [Cunha Alves, Alfredo Augusto] USDA ARS, Embrapa LABEX, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80522 USA. [Ribeiro dos Santos, Francisco de Assis] Univ Estadual Feira de Santana, Feira De Santana, BA, Brazil. [Duarte Souzas, Fernanda Vidigal] Embrapa Mandioca & Fruticultura, Cruz Das Almas, BA, Brazil. RP Vieira, LD (reprint author), Univ Fed Reconcavo Bahia, Cruz Das Almas, BA, Brazil. EM liviabiol@gmail.com RI Santos, Francisco/D-3661-2009 OI Santos, Francisco/0000-0002-9246-3146 FU Capes; CNPq; Embrapa Cassava Fruits; Generation Challenge Programme (GCP) FX To Capes, for awarding the Master's fellowship to LJV; to CNPq for the productivity in Science grant awarded to FARS. To Embrapa Cassava & Fruits and Generation Challenge Programme (GCP) for funding the projects that enabled this study. Thanks to Professor Elliot Watanabe Kitajima (NAP/MEPA-ESALQ/USP) for the SEM facilities. NR 22 TC 2 Z9 2 U1 0 U2 7 PU SOC BOTANICA BRASIL PI SAO PAULO SP PA CAIXA POSTAL 3005, SAO PAULO SP, 01061-970, BRAZIL SN 0102-3306 J9 ACTA BOT BRAS JI Acta Bot. Bras. PD APR-JUN PY 2012 VL 26 IS 2 BP 350 EP 356 PG 7 WC Plant Sciences SC Plant Sciences GA 972GW UT WOS:000306266300011 ER PT J AU Saenz-Romero, C Rehfeldt, GE Soto-Correa, JC Aguilar-Aguilar, S Zamarripa-Morales, V Lopez-Upton, J AF Saenz-Romero, Cuauhtemoc Rehfeldt, Gerald E. Carmen Soto-Correa, Jose Aguilar-Aguilar, Selene Zamarripa-Morales, Veronica Lopez-Upton, Javier TI ALTITUDINAL GENETIC VARIATION AMONG Pinus pseudostrobus POPULATIONS FROM MICHOACAN, MEXICO. TWO LOCATION SHADEHOUSE TEST RESULTS SO REVISTA FITOTECNIA MEXICANA LA English DT Article DE Pinus pseudostrobus; altitudinal genetic variation; altitudinal seed zoning; assisted migration; climatic change ID CLIMATE-CHANGE; FROST DAMAGE; DOUGLAS-FIR; FORESTS; GROWTH; USA; MANAGEMENT; MORTALITY; DROUGHT; MODEL AB Pinus pseudostrobus Lindl. is the forest species most important economically in the state of Michoacan, at central-west Mexico. We investigated genetic variation among P. pseudostrobus populations along an altitudinal gradient in the native indian community of Nuevo San Juan Parangaricutiro, Michoacan, Mexico. Cones were collected from eight populations at 100 m of altitudinal intervals between 2200 m and 2900 m. Seedlings were grown for approximately two years in two shadehouse environments at Morelia, Michoacan, Mexico and at Moscow, Idaho, USA. Total height was periodically measured during the second growing season to estimate a series of growth indexes for both locations, and dry weights were obtained only for the Morelia test. For the Moscow test there were significant differences (P < 0.05) among populations for cessation of growth, duration of the growth period, and final height. For the Morelia tests there were differences among populations for foliage, shoot and total dry weight (P < 0.025), and significant differences for stem dry weight at P = 0.055 level. There was a strong altitudinal dine for dry weight variables, with seedlings originated from populations from the lowest altitudes having more biomass (r(2) = 0.80, P = 0.003). Populations separated altitudinally by about 295 m are likely to be genetically different. We suggest delineation of three altitudinal seed zones (Zone I: 2100 m to 2400 m; Zone II: 2400 m to 2700 m; Zone III: 2700 m to 3000 m), that can be translated into climatic zones delimited according to mean annual temperatures or by degree days > 5 degrees C. To accommodate climate change we suggest implementing assisted migration programs by transferring populations 300 m upwards to maintain adaptiveness of populations for future climates. C1 [Saenz-Romero, Cuauhtemoc; Carmen Soto-Correa, Jose] Univ Michoacana San Nicolas Hidalgo IIAF UMSNH, Inst Invest Agropecuarias & Forestales, Tarimbaro, Michoacan, Mexico. [Rehfeldt, Gerald E.] US Forest Serv, Forestry Sci Lab, Mt Res Stn, USDA, Moscow, ID 83843 USA. [Aguilar-Aguilar, Selene; Zamarripa-Morales, Veronica] Univ Michoacana, Fac Biol, Morelia, Michoacan, Mexico. [Lopez-Upton, Javier] Colegio Postgrado, Texcoco, Estado De Mexic, Mexico. RP Saenz-Romero, C (reprint author), Univ Michoacana San Nicolas Hidalgo IIAF UMSNH, Inst Invest Agropecuarias & Forestales, Km 9-5 Carretera Morelia Zinapecuaro 58880, Tarimbaro, Michoacan, Mexico. EM csaenzromero@gmail.com FU Mexican Council of Science and Technology; Mexican National Forestry Commission [CONACYT-SIMORELOS-2000-0306021, CONACYT-CONAFOR-2002-C01-4655]; State of Michoacan [CONACYT-MICHOACAN-2009-127128]; Coordinacion de la Investigacion Cientifica of the Universidad Michoacana de San Nicolas de Hidalgo [5.1]; USDA- Forest Service, Rocky Mountain Research Station [01-JV-11222063-183] FX Funding was provided to CSR by Mexican Council of Science and Technology and the Mexican National Forestry Commission (CONACYT-SIMORELOS-2000-0306021 and CONACYT-CONAFOR-2002-C01-4655), the State of Michoacan (CONACYT-MICHOACAN-2009-127128), the Coordinacion de la Investigacion Cientifica of the Universidad Michoacana de San Nicolas de Hidalgo (5.1) and the USDA- Forest Service, Rocky Mountain Research Station (01-JV-11222063-183). Thanks to Manuel Echeverria, Rafael Echeverria, Luis Toral, Felipe Aguilar and other persons from the Forestry Office of the native indian community of Nuevo San Juan Parangaricutiro, Michoacan, and to Ernesto Moreno, Daniel Saldivar, Victor Quinonez and other personnel of the Michoacan State Forest Commission for their help with seed collection. We thank Patrick Wells at Moscow and Soraya Gonzalez and Guadalupe Hernandez at Morelia for their help for experiments maintenance, and to Hans Nienstaedt for valuable comments. NR 29 TC 11 Z9 12 U1 0 U2 6 PU SOC MEXICANA FITOGENETICA PI CHAPINGO PA APARTADO POSTAL NO 21, CHAPINGO, ESTADO MEXICO 56 230, MEXICO SN 0187-7380 J9 REV FITOTEC MEX JI Rev. Fitotec. Mex. PD APR-JUN PY 2012 VL 35 IS 2 BP 111 EP 121 PG 11 WC Agronomy; Horticulture SC Agriculture GA 972TY UT WOS:000306304600002 ER PT J AU Longland, WS AF Longland, William S. TI Small Mammals in Saltcedar (Tamarix ramosissima)-Invaded and Native Riparian Habitats of the Western Great Basin SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Heteromyidae; native; nonnative; riparian woodlands; rodents ID RIO-GRANDE VALLEY; LOWER COLORADO RIVER; NEW-MEXICO; UNITED-STATES; VEGETATION; TAMARIX; COMMUNITIES; ARIZONA; BIRDS; USA AB Invasive saltcedar species have replaced native riparian trees on numerous river systems throughout the western United States, raising concerns about how this habitat conversion may affect wildlife. For periods ranging from 2 to 11 yr, I used live-trapping to monitor small mammal populations in paired saltcedar and native riparian woodlands at four sites in western Nevada and eastern California. Heteromyid rodents, such as Merriam's and Ord's kangaroo rats, were more likely to occur in saltcedar habitats, but other rodent species, particularly the montane vole and western harvest mouse, occurred more often in native habitats, and this balanced species richness in habitat comparisons. The most common species at all sites, the deer mouse, did not show any consistent differences in abundance or in mean body mass between the two habitat types. However, the ratio of captured male to female deer mice was higher in saltcedar than native habitats at two sites. Deer mice as well as Ord's kangaroo rats also had higher rates of being recaptured following initial capture in native habitats, which may have been due to fewer transient individuals occurring in these habitats. By contrast, Merriam's kangaroo rats may have been more transient in native habitats because they were more likely to be recaptured in saltcedar. Individuals of two species, pinyon mouse and white-tailed antelope ground squirrel, had greater mean body mass in native habitats than they did in saltcedar, implying that they may have maintained superior condition in native habitats. C1 ARS, Great Basin Rangelands Res Unit, USDA, Reno, NV 89512 USA. RP Longland, WS (reprint author), ARS, Great Basin Rangelands Res Unit, USDA, 920 Valley Rd, Reno, NV 89512 USA. EM longland@unr.edu NR 30 TC 4 Z9 4 U1 2 U2 19 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2012 VL 5 IS 2 BP 230 EP 237 DI 10.1614/IPSM-D-11-00019.1 PG 8 WC Plant Sciences SC Plant Sciences GA 967AZ UT WOS:000305879800010 ER PT J AU Matlaga, DP Schutte, BJ Davis, AS AF Matlaga, David P. Schutte, Brian J. Davis, Adam S. TI Age-Dependent Demographic Rates of the Bioenergy Crop Miscanthus X giganteus in Illinois SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Miscanthus X giganteus; biofeedstock; Illinois; rhizome establishment ID AUSTRALIS COMMON REED; DONAX GIANT REED; PHRAGMITES-AUSTRALIS; ARUNDO-DONAX; PHALARIS-ARUNDINACEA; CHESAPEAKE BAY; COASTAL MARSH; UNITED-STATES; ENERGY CROPS; SINENSIS AB Some plants being considered as bioenergy crops share traits with invasive species and have histories of spreading outside of their native ranges, highlighting the importance of evaluating the invasive potential before the establishment of large-scale plantings. The Asian grass Miscanthus X giganteus is currently being planted as a bioenergy crop in the north central region of the United States. Our goal was to understand the demographic rates and vegetative spread of this species in unmanaged arable lands in Illinois to compare with those of large-statured invasive grasses (LSIGs). We collected data from 13 M. X giganteus plantings in Illinois, ranging in age from 1 to 7 yr, recording tiller number, plant spatial extent, spikelet production, and plant survival over 4 yr. Additionally, to understand recruitment potential, we conducted a greenhouse germination experiment, and, to estimate establishment from rhizome fragments, field trials were performed. Miscanthus X giganteus demographic rates were age dependent. Spikelet production was high, with 1- and 4-yr plants producing an annual average of more than 10,000 and 180,000 spikelets plant(-1), respectively; however, data from our germination trial suggested that none of these spikelets had the potential to yield seedlings. On average, plants expanded vegetatively 0.15 m yr(-1). Tiller density within the center of a clone decreased with age, possibly leading to a "dead center" found among some LSIGs. Rhizome establishment increased with weight, ranging from 0 to 42%. Survival was low, 24%, for first-year plants but quickly climbed to an asymptote of 98% survival for 4-yr-old plants. Our results suggest that efforts should be made to eradicate plants that escape biomass production fields within a year of establishment, before the onset of high survival. Future work is needed to determine what types of natural and anthropogenic disturbances can fragment rhizomes, leading to regeneration. C1 [Matlaga, David P.; Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Washington, DC 20250 USA. [Schutte, Brian J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Matlaga, DP (reprint author), USDA ARS, Global Change & Photosynth Res Unit, Washington, DC 20250 USA. EM dmatlaga@illinois.edu NR 61 TC 11 Z9 11 U1 1 U2 25 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2012 VL 5 IS 2 BP 238 EP 248 DI 10.1614/IPSM-D-11-00083.1 PG 11 WC Plant Sciences SC Plant Sciences GA 967AZ UT WOS:000305879800011 ER PT J AU Conn, JS AF Conn, Jeffery S. TI Pathways of Invasive Plant Spread to Alaska: III. Contaminants in Crop and Grass Seed SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Pathways; Alaska; invasion prevention; crop seed; grass seed; other crop species; seed law; seed contaminants ID WEED FLORA AB Invasive plants disperse to new areas via numerous pathways. Study of these pathways helps to focus limited budgets toward prevention and early detection. This study examined potentially invasive seed contaminants in imported crops and grass seed as pathways for plant dispersal to Alaska. Crop and grass seed were purchased from 13 Alaska retail outlets representing 14 seed suppliers. Seed bags were sampled using federally mandated protocols and were analyzed for crop seeds that were not supposed to be included and for weed contaminants. Ninety-five weed and 36 contaminant crop taxa were found. Crop seed contained 43 weed taxa and 15 other crop species contaminants, a mean of 6.4 taxa and 3,844 contaminant seed kg(-1). Grass seed samples contained 73 weed taxa and 21 crop contaminants, a mean of 3.5 contaminant species and 1,250 seeds kg(-1). Two species prohibited by the Alaska seed law were found: Canada thistle was found in a single crop sample, and quackgrass was found in two grass samples. There were no significant relationships between either seed type or supplier and either the number of contaminant species or number of seeds. Labels of 33% of crop samples and 8% of grass samples claimed 0.00% weed seeds, but low (0.007% by weight, 2 species) to high (1.18% by weight, 13 species) amounts of weed contaminants were found. Importation of crop seed is a large pathway for seed movement, causing significant propagule pressure and an increased likelihood of establishment by new invasive plant populations. Prevention of spread via this pathway would be enhanced by changes to seed laws, by greater regulatory enforcement, and by including on the label, the names of all weed and contaminant crop species found in the law-required samples. Consumers could then make decisions on whether to purchase seed based on the potentially invasive species that would be planted with the desired seed. C1 Univ Alaska, Agr Res Unit, ARS, USDA, Fairbanks, AK 99775 USA. RP Conn, JS (reprint author), Univ Alaska, Agr Res Unit, ARS, USDA, 319 ONeill Bldg, Fairbanks, AK 99775 USA. EM jeff.conn@ars.usda.gov NR 35 TC 2 Z9 2 U1 2 U2 10 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2012 VL 5 IS 2 BP 270 EP 281 DI 10.1614/IPSM-D-11-00073.1 PG 12 WC Plant Sciences SC Plant Sciences GA 967AZ UT WOS:000305879800014 ER PT J AU Wang, ZY Li, RY Xu, JL Marita, JM Hatfield, RD Qu, RD Cheng, JJ AF Wang, Ziyu Li, Ruyu Xu, Jiele Marita, Jane M. Hatfield, Ronald D. Qu, Rongda Cheng, Jay J. TI Sodium hydroxide pretreatment of genetically modified switchgrass for improved enzymatic release of sugars SO BIORESOURCE TECHNOLOGY LA English DT Article DE Genetic modification; Lignin; Switchgrass; Sodium hydroxide pretreatment; Sugar ID BIOFUEL PRODUCTION; LIGNIN BIOSYNTHESIS; ETHANOL-PRODUCTION; CELLULOSIC ETHANOL; BERMUDA GRASS; HYDROLYSIS; RECALCITRANCE; TECHNOLOGIES; BIOETHANOL; OXIDATION AB Overcoming biomass recalcitrance to bioconversion is crucial for cellulosic biofuels commercialization. In this study, Alamo switchgrass (Panicum virgatum L.) was genetically transformed to suppress the expression of 4-coumarate-CoA ligase (4CL). The transgenic plants were determined to have lignin content reductions of up to 5.8%. The ratios of acid soluble lignin (ASL) to acid insoluble lignin (AIL) and syringyl/guaiacyl (S/G) in transgenic plants were 21.4-64.3% and 11.8-164.5%, respectively, higher than those of conventional biomass. Both conventional and transgenic plants were pretreated with 0.5%, 1%, and 2% (w/v) NaOH for 15, 30, and 60 min at 121 degrees C, followed by enzymatic hydrolysis with commercial cellulases and xylanases. At the optimal conditions, the glucan and xylan conversion efficiency in the best transgenic plants were 16% and 18% higher than the conventional plant, respectively. The results show that down-regulation of 4CL gene promoted enzymatic hydrolysis of plant cell walls following a mild alkali pretreatment. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Cheng, Jay J.] N Carolina State Univ, Dept Biol & Agr Engn, Weaver Labs 275, Raleigh, NC 27695 USA. [Li, Ruyu; Qu, Rongda] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Marita, Jane M.; Hatfield, Ronald D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Cheng, JJ (reprint author), N Carolina State Univ, Dept Biol & Agr Engn, Weaver Labs 275, Raleigh, NC 27695 USA. EM jay_cheng@ncsu.edu FU US Department of Energy through Consortium for Plant Biotechnology Research, Inc. FX This work was funded by the US Department of Energy through Consortium for Plant Biotechnology Research, Inc. The authors would like to thank Dr. Dhana Savithri in the Integrated Biomass Research Initiative Laboratory of North Carolina State University for her help on the HPAE analysis. NR 35 TC 18 Z9 22 U1 1 U2 35 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD APR PY 2012 VL 110 BP 364 EP 370 DI 10.1016/j.biortech.2012.01.097 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 966QO UT WOS:000305852700053 PM 22330602 ER PT J AU Bowman, MJ Dien, BS Hector, RE Sarath, G Gotta, MA AF Bowman, Michael J. Dien, Bruce S. Hector, Ronald E. Sarath, Gautam Gotta, Michael A. TI Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation SO BIORESOURCE TECHNOLOGY LA English DT Article DE HILIC-MS/MS; Switchgrass; Xylan; Xylooligosaccharide; SSF ID ASSISTED-LASER-DESORPTION/IONIZATION; ETHANOL FERMENTATION; ACID; HYDROLYSIS; XYLAN; OLIGOSACCHARIDES; INHIBITORS; CONVERSION; ALFALFA; AFEX AB Switchgrass is a potential source of renewable biomass for conversion to liquid biofuels. Efficient conversion requires effective strategies for pretreatment and enzymatic saccharification to produce fermentable sugars. Standard analysis of fermentation liquids includes detection of monosaccharides and ethanol to determine efficiency of conversion. Larger components, specifically oligosaccharides, are typically not measured due to the structural complexity of the products; however, as oligosaccharides they represent carbon available in biomass that is not converted to liquid fuels. In this study, ammonia-pretreated switchgrass was enzymatically depolymerized either independently or under simultaneous saccharification and fermentation conditions. Residual oligosaccharides were reducing end-labeled followed by hydrophilic interaction liquid chromatography mass spectrometry/mass spectrometry analysis. These data reveal 20 oligosaccharide peaks with distinct retention times and tandem mass spectrometry fragmentation patterns representing 13 different oligosaccharide compositions. All measured compositions were smaller than a chain length of six and were neither linear xylooligosaccharides nor modified with phenolic esters. This work represents a robust method to monitor and identify unhydrolyzed oligosaccharides from fermentations, thereby permitting the screening of targeted enzymatic activities to promote the complete hydrolysis of xylan. Published by Elsevier Ltd. C1 [Bowman, Michael J.; Dien, Bruce S.; Hector, Ronald E.; Gotta, Michael A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. [Sarath, Gautam] ARS, USDA, Cent E Reg Biomass Ctr, UNL, Lincoln, NE 68583 USA. RP Bowman, MJ (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.bowman@ars.usda.gov OI Cotta, Michael/0000-0003-4565-7754; Dien, Bruce/0000-0003-3863-6664 NR 38 TC 5 Z9 5 U1 2 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD APR PY 2012 VL 110 BP 437 EP 447 DI 10.1016/j.biortech.2012.01.062 PG 11 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 966QO UT WOS:000305852700063 PM 22330596 ER PT J AU McCullough, PE Schwartz, BM Grey, T Webster, T AF McCullough, Patrick E. Schwartz, Brian M. Grey, Timothy Webster, Theodore TI Preemergence Herbicides Influence Sprig Establishment of 'TifEagle' Bermudagrass SO WEED TECHNOLOGY LA English DT Article DE Bermudagrass; preemergence; putting green; sprigs ID ZOYSIAGRASS ZOYSIA-JAPONICA; DIMETHENAMID-P; TOLERANCE; METOLACHLOR; CULTIVARS; OXADIAZON; TURF AB The presence of weeds during bermudagrass putting green establishment can reduce growth and turf quality. Three field experiments were conducted in Georgia to investigate efficacy of dimethenamid, S-metolachlor, and oxadiazon on the establishment of 'TifEagle' bermudagrass from sprigs. Dimethenamid at 0.85 and 1.7 kg ai ha(-1) S-metolachlor at 1.1 and 2.2 kg ai ha(-1), and oxadiazon at 1.1, 2.2, and 4.4 kg ai ha(-1) did not reduce bermudagrass cover from the untreated after 8 wk. S-metolachlor at 4.4 kg ha-1 was the only treatment that reduced sprig cover from the untreated after 12 wk. All S-metolachlor and oxadiazon treatments provided excellent (>= 90%) green kyllinga control by 8 wk after treatment (WAT) while dimethenamid at 0.85, 1.7, and 3.4 kg ha(-1) provided 78, 85, and 92% control, respectively. Dimethenamid treatments provided poor control (< 70%) of spotted spurge but fair control (70 to 79%) was achieved from S-metolachlor at 4.4 kg ha(-1) and oxadiazon at 2.2 and 4.4 kg ha(-1) by 8 WAT. Overall, low to middle rates of the herbicides tested appear to temporarily inhibit TifEagle bermudagrass sprig establishment but high rates of dimethenamid and S-metolachlor may reduce cover from the untreated. C1 [McCullough, Patrick E.] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA USA. Univ Georgia, Dept Crop & Soil Sci, Tifton, GA USA. ARS, Crop Protect & Management Res Unit, USDA, Tifton, GA USA. RP McCullough, PE (reprint author), Univ Georgia, Dept Crop & Soil Sci, Griffin, GA USA. EM pmccull@uga.edu RI Webster, Theodore/A-4468-2009 OI Webster, Theodore/0000-0002-8259-2059 NR 33 TC 2 Z9 2 U1 0 U2 4 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2012 VL 26 IS 2 BP 300 EP 303 DI 10.1614/WT-D-11-00151.1 PG 4 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 964VW UT WOS:000305725800021 ER PT J AU Johnson, WC Boudreau, MA Davis, JW AF Johnson, W. Carroll, III Boudreau, Mark A. Davis, Jerry W. TI Implements and Cultivation Frequency to Improve In-Row Weed Control in Organic Peanut Production SO WEED TECHNOLOGY LA English DT Article DE Brush hoe; cultivation; mechanical weed control; organic weed control; tine weeder ID MANAGEMENT; HYPOGAEA; ARACHIS; SYSTEMS AB Weed control in organic peanut production is difficult and costly. Sweep cultivation in the row middles is effective, but weeds remain in the crop row, causing yield loss. Research trials were conducted in Ty Ty, GA to evaluate implements and frequencies of cultivation to improve in-row weed control in organic peanut. Implements were a tine weeder and power takeoff-powered brush hoe that targeted weeds present in the row. Frequencies of cultivation were at vegetative emergence of peanut (VE), 1 wk after VE (1wk), 2 wk after VE (2wk), sequential combinations of VE/1wk, VE/2wk, and VE/1wk/2wk. All plots were cultivated with a sweep cultivator to control weeds in row middles. The tine weeder tended to be easier to operate and performed more consistently than the brush hoe. Both implements performed best when initial cultivation was at VE. Delaying the initial cultivation reduced overall effectiveness. Plots with the best in-row weed control were hand-weeded once to control escapes and harvested for peanut yield. The best overall combination of weed control, minimal use of salvage hand-weeding, and maximum peanut yield resulted from sequential cultivation at VE/1wk using either the tine weeder or brush hoe, row middle sweep cultivation, and preharvest mowing. C1 [Johnson, W. Carroll, III] USDA ARS, Tifton, GA 31793 USA. Univ Georgia, Dept Biol & Agr Engn, Athens, GA 30602 USA. Univ Georgia, Griffin, GA 30223 USA. RP Johnson, WC (reprint author), USDA ARS, POB 748, Tifton, GA 31793 USA. EM Carroll.Johnson@ars.usda.gov FU Southern Region Sustainable Agricultural Res. and Education Program (Southern SARE), through Hebert Green Agroecology, Inc. FX We acknowledge the contributions of Daniel R. Evarts and Terrell W. Jones, whose technical skills, mechanical insights, and improvisational abilities made these studies possible. Appreciation is also expressed to the Reforestation Department, Georgia Forestry Commission for the use of their brush-hoe cultivator. This research was partially supported by a grant from the Southern Region Sustainable Agricultural Res. and Education Program (Southern SARE), through Hebert Green Agroecology, Inc. NR 18 TC 5 Z9 7 U1 1 U2 6 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2012 VL 26 IS 2 BP 334 EP 340 DI 10.1614/WT-D-11-00142.1 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 964VW UT WOS:000305725800026 ER PT J AU Hwang, HS Kim, S Singh, M Winkler-Moser, JK Liu, SX AF Hwang, Hong-Sik Kim, Sanghoon Singh, Mukti Winkler-Moser, Jill K. Liu, Sean X. TI Organogel Formation of Soybean Oil with Waxes SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Organogel; Wax; Wax ester; Sunflower wax; Soybean oil; Trans-fat; Saturated fat ID CANDELILLA WAX; DOTRIACONTANE ORGANOGELS; FATTY ALCOHOLS; SAFFLOWER OIL; ACIDS; ESTERS AB Many waxes including plant waxes and animal waxes were evaluated for the gelation ability toward soybean oil (SBO) and compared with hydrogenated vegetable oils, petroleum waxes and commercial non-edible gelling agents to understand factors affecting the gelation ability of a gelator. Sunflower wax (SW) showed the most promising results and all SW samples from three different suppliers could make a gel with concentrations as low as 0.5 wt%. Candelilla wax and rice bran wax also showed good gelation properties, which, however, varied with different suppliers. Gelation ability of a wax is significantly dependant on its purity and detailed composition. A wax ester with longer alkyl chains has significantly better gelation ability toward SBO than that with shorter alkyl chains indicating that the chain length of a component in a wax such as wax ester is an important factor for gelation ability. The SW-SBO organogel showed increased melting point with increased SW content, showing the melting point range from about 47 to 65 degrees C with 0.5-10 wt% SW. The effects of cooling rate on crystal size and firmness of a gel were investigated. The dependence of firmness on cooling rate was so significant that the desired texture of an organogel could be achieved by controlling the cooling rate in addition to controlling the amount of gelling agent. This research reveals that a small amount of food grade plant waxes including SW may replace a large amount of the hardstock containing trans-fat or saturated fat. C1 [Hwang, Hong-Sik; Kim, Sanghoon; Singh, Mukti; Winkler-Moser, Jill K.; Liu, Sean X.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Hwang, HS (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815N Univ St, Peoria, IL 61604 USA. EM Hongsik.hwang@ars.usda.gov NR 24 TC 38 Z9 38 U1 6 U2 60 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD APR PY 2012 VL 89 IS 4 BP 639 EP 647 DI 10.1007/s11746-011-1953-2 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 959DR UT WOS:000305292300010 ER PT J AU Jackson, MA Appell, M Berhow, MA Blackburn, JA Rheiner, SN AF Jackson, Michael A. Appell, Michael Berhow, Mark A. Blackburn, Judith A. Rheiner, Steven N. TI The Acrylation of Glycerol: A Precursor to Functionalized Lipids SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Acrylation; Heck reaction; Functionalized lipids; Base catalysis; Thioether; Michael addition ID LIPASE-CATALYZED TRANSESTERIFICATION; OIL; ACIDS AB With the objective of expanding the number of functionalized lipids available, the reactive vinyl group of acrylic acid was introduced to triacylglycerol by esterification of glycerol. Didecanoylacryloylglycerol was synthesized from decanoic and acrylic acids and glycerol using K2O as catalyst. 2 g (21.7 mmol) glycerol, 11.3 g (65.4 mmol) decanoic acid, 6.2 g (86.1 mmol) acrylic acid, 60 ml hexane, and 400 mg K2O were added to 300-ml closed stainless steel reactor and maintained at 200 degrees C for 5 h. The resulting product, designated DDA, was isolated at about 40% yield based on the acylglycerol products. The other products included tridecanoylglycerol, diacryloyldecanoylglycerol, and the diacylglycerols of these acids. DDA was then converted to functionalized lipids by the Michael addition and Heck reaction. The Michael addition of thiophenol and 4-bromothiophenol yielded the corresponding linear thioethers whereas and Heck reaction products from bromobenzene and bromoanisole yielded triacylglycerols containing trans-cinnamic acid and trans(4-methoxy)cinnamic acid, respectively. C1 [Jackson, Michael A.; Blackburn, Judith A.; Rheiner, Steven N.] ARS, Renewable Prod Technol Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Berhow, Mark A.] ARS, Funct Foods Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Jackson, MA (reprint author), ARS, Renewable Prod Technol Unit, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.jackson@ars.usda.gov NR 20 TC 1 Z9 1 U1 4 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD APR PY 2012 VL 89 IS 4 BP 713 EP 719 DI 10.1007/s11746-011-1950-5 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 959DR UT WOS:000305292300018 ER PT J AU Liu, SX Rexroad, CE Couch, CR Cordes, JF Reece, KS Sullivan, CV AF Liu, Sixin Rexroad, Caird E., III Couch, Charlene R. Cordes, Jan F. Reece, Kimberly S. Sullivan, Craig V. TI A Microsatellite Linkage Map of Striped Bass (Morone saxatilis) Reveals Conserved Synteny with the Three-Spined Stickleback (Gasterosteus aculeatus) SO MARINE BIOTECHNOLOGY LA English DT Article DE Striped bass; Microsatellite; Linkage map; Synteny; Three-spined stickleback ID TROUT ONCORHYNCHUS-MYKISS; DICENTRARCHUS-LABRAX L; SHORT-TAILED OPOSSUM; CELLULAR-DNA CONTENT; SEA BASS; RECOMBINATION RATES; GENETIC-MAP; PARALICHTHYS-OLIVACEUS; MONODELPHIS-DOMESTICA; COMPARATIVE GENOMICS AB The striped bass (Morone saxatilis) and its relatives (genus Morone) are of great importance to fisheries and aquaculture in North America. As part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. The objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (Gasterosteus aculeatus). Of 480 microsatellite markers screened for polymorphism, 289 informative markers were identified and used to genotype two half-sib mapping families. Twenty-six linkage groups were assembled, and only two markers remain unlinked. The sex-averaged map spans 1,623.8 cM with an average marker density of 5.78 cM per marker. Among 287 striped bass microsatellite markers assigned to linkage groups, 169 (58.9%) showed homology to sequences on stickleback chromosomes or scaffolds. Comparison between the stickleback genome and the striped bass linkage map revealed conserved synteny between these two species. This is the first linkage map for any of the Morone species. This map will be useful for molecular mapping and marker-assisted selection of genes of interest in striped bass breeding programs. The conserved synteny between striped bass and stickleback will facilitate fine mapping of genome regions of interest and will serve as a new resource for comparative mapping with other Perciform fishes such as European sea bass (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), and tilapia (Oreochromis ssp.). C1 [Liu, Sixin; Rexroad, Caird E., III] ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Couch, Charlene R.] N Carolina State Univ, Dept Genet, Raleigh, NC 27695 USA. [Cordes, Jan F.; Reece, Kimberly S.] Virginia Inst Marine Sci, Dept Environm & Aquat Anim Hlth, Gloucester Point, VA 23062 USA. [Sullivan, Craig V.] N Carolina State Univ, Dept Biol, Raleigh, NC 27695 USA. RP Liu, SX (reprint author), ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. EM sixin.liu@ars.usda.gov RI Sullivan, Craig/B-3863-2014 OI Sullivan, Craig/0000-0002-3609-9458 FU Marine Aquaculture Initiative from the National Oceanic and Atmospheric Administration [NAO60AR4170250] FX The authors would like to thank Guangtu Gao for help with the software MULTIMAP, and Alanna MacIntyre and Georgeta Constantin for their technical assistance. We also thank Amber Garber, Mark Westerman, Andy McGinty, and Michael Hopper for help with marker and/or mapping family development. This project was funded by a Marine Aquaculture Initiative grant NAO60AR4170250 to CVS from the National Oceanic and Atmospheric Administration. The VIMS contribution number is 3180. NR 53 TC 10 Z9 10 U1 2 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-2228 EI 1436-2236 J9 MAR BIOTECHNOL JI Mar. Biotechnol. PD APR PY 2012 VL 14 IS 2 BP 237 EP 244 DI 10.1007/s10126-011-9407-2 PG 8 WC Biotechnology & Applied Microbiology; Marine & Freshwater Biology SC Biotechnology & Applied Microbiology; Marine & Freshwater Biology GA 958FF UT WOS:000305221200010 PM 21968826 ER PT J AU Shelton, TG AF Shelton, T. G. TI Assessment of Donor/Recipient Ratios in Permethrin Transfer Studies with Reticulitermes virginicus Banks SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE toxicant transfer; Reticulitermes; permethrin; pyrethroids ID SUBTERRANEAN TERMITE ISOPTERA; COPTOTERMES-FORMOSANUS SHIRAKI; NONREPELLENT TERMITICIDES; HORIZONTAL TRANSFER; FLAVIPES ISOPTERA; REMEDIAL CONTROL; RHINOTERMITIDAE; TOXICITY; FIPRONIL; WORKERS AB Crowding of termites may influence observed recipient mortality in donor-recipient toxicant transfer studies. It was hypothesized that if crowding were important, any termiticide (particularly repellent termiticides, which were not thought to transfer) could be transferred by contact among termites when the donor complement was sufficient. Termiticide transfer donor-recipient studies were performed with 5 ppm permethrin (a repellent termiticide; 30 min exposure time) in donor: recipient ratios of 10:90, 20:80, 30:70, 40:60, and 50:50 using 4 colonies of Reticulitermes virginicus Banks. Recipient mortality at 14 d was significantly different between treatments and controls in the 30:70 ratio for 3 colonies. Treatments and controls were significantly different for recipient mortality of one colony each in the 40:60 and 50:50 donor: recipient ratios. These results demonstrated that a repellent termiticide could be transferred by contact among termites with high donor percentages; they suggest that the phenomenon of toxicant transfer by contact may be influenced by crowding in these studies. C1 US Forest Serv, USDA, So Res Stn, Starkville, MS 39759 USA. RP Shelton, TG (reprint author), US Forest Serv, USDA, So Res Stn, 201 Lincoln Green, Starkville, MS 39759 USA. EM tshelton@fs.fed.us NR 41 TC 2 Z9 2 U1 0 U2 3 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2012 VL 47 IS 2 BP 139 EP 149 PG 11 WC Entomology SC Entomology GA 953SI UT WOS:000304893100005 ER PT J AU Lundgren, JG Harwood, JD AF Lundgren, Jonathan G. Harwood, James D. TI Functional Responses to Food Diversity: the Effect of Seed Availability on the Feeding Behavior of Facultative Granivores SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE dietary breadth; granivory; gut content analysis; omnivory; predator ID GROUND BEETLES COLEOPTERA; DIEL ACTIVITY PATTERNS; ALTERNATIVE PREY; CROP-ROTATION; CARABIDAE ASSEMBLAGES; GENERALIST PREDATORS; SETARIA-FABERI; HARVESTER ANTS; COMMUNITY; OMNIVORY AB We examined how diverse communities of carabid beetles and crickets in a perennial hayfield respond to seed availability numerically and in their feeding behavior. Although there were 3 distinct insect communities identified over the diel cycle, these communities were similar in plots supplemented with Setaria viridis (L.) P. Beauv. seeds and untreated plots. Gryllus pennsylvanicus Burmeister consumed plant material more frequently and ate more food in the fields with greater seed availability. However, they consumed prey with equal frequency in the 2 treatments. Allonemobius sp. consumed food less frequently than G. pennsylvanicus, and their diet was unaffected by seed availability. Availability of nonprey food resources may not affect soil insect communities in the short term, but some omnivores are quick to alter their diet to exploit nonprey resources. C1 [Lundgren, Jonathan G.] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. [Harwood, James D.] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA. RP Lundgren, JG (reprint author), ARS, USDA, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM Jonathan.Lundgren@ars.usda.gov FU University of Kentucky Agricultural Experiment Station [KY008043] FX The authors thank Janet Fergen for supervising this project and conducting the immunoassays; Carson Dinger, Mallory Johnson, and Lucia Pilorget for providing technical assistance; and Ricardo Bessin and Paula Westerman for reviewing earlier drafts of this manuscript. Mention of any proprietary products does not constitute endorsement by the USDA. James Harwood is supported by the University of Kentucky Agricultural Experiment Station State Project KY008043. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 80 TC 7 Z9 7 U1 5 U2 37 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2012 VL 47 IS 2 BP 160 EP 176 PG 17 WC Entomology SC Entomology GA 953SI UT WOS:000304893100007 ER PT J AU Carroll, JF Kramer, M AF Carroll, J. F. Kramer, M. TI Responses of Lone Star Tick (Acari: Ixodidae) Nymphs to the Repellent Deet Applied in Acetone and Ethanol Solutions in In Vitro Bioassays SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Amblyomma americanum; behavioral bioassay; dose response; drying time C1 ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Carroll, JF (reprint author), Biometr Consulting Serv, Invas Insect Biocontrol & Behav Lab, Washington, DC 20250 USA. EM john.carroll@ars.usda.gov NR 0 TC 1 Z9 1 U1 0 U2 5 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2012 VL 47 IS 2 BP 193 EP 196 PG 4 WC Entomology SC Entomology GA 953SI UT WOS:000304893100011 ER PT J AU Robinson, SJ Samuel, MD O'Rourke, KI Johnson, CJ AF Robinson, Stacie J. Samuel, Michael D. O'Rourke, Katherine I. Johnson, Chad J. TI The role of genetics in chronic wasting disease of North American cervids SO PRION LA English DT Review DE cervids; chronic wasting disease; disease progression; susceptibility; prion disease; prion protein gene (Prnp) ID WHITE-TAILED DEER; TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES; CREUTZFELDT-JAKOB-DISEASE; CERVUS-ELAPHUS-NELSONI; RESISTANT PRION PROTEIN; RANGING MULE DEER; ODOCOILEUS-HEMIONUS; INFLUENCE SUSCEPTIBILITY; ORAL-TRANSMISSION; SPECIES BARRIERS AB Chronic wasting disease (CWD) is a major concern for the management of North American cervid populations. This fatal prion disease has led to declines in populations which have high CWD prevalence and areas with both high and low infection rates have experienced economic losses in wildlife recreation and fears of potential spill-over into livestock or humans. Research from human and veterinary medicine has established that the prion protein gene (Prnp) encodes the protein responsible for transmissible spongiform encephalopathies (TSEs). Polymorphisms in the Prnp gene can lead to different prion forms that moderate individual susceptibility to and progression of TSE infection. Prnp genes have been sequenced in a number of cervid species including those currently infected by CWD (elk, mule deer, white-tailed deer, moose) and those for which susceptibility is not yet determined (caribou, fallow deer, sika deer). Over thousands of sequences examined, the Prnp gene is remarkably conserved within the family Cervidae; only 16 amino acid polymorphisms have been reported within the 256 amino acid open reading frame in the third exon of the Prnp gene. Some of these polymorphisms have been associated with lower rates of CWD infection and slower progression of clinical CWD. Here we review the body of research on Prnp genetics of North American cervids. Specifically, we focus on known polymorphisms in the Prnp gene, observed genotypic differences in CWD infection rates and clinical progression, mechanisms for genetic TSE resistance related to both the cervid host and the prion agent and potential for natural selection for CWD-resistance. We also identify gaps in our knowledge that require future research. C1 [Robinson, Stacie J.] Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53715 USA. [Samuel, Michael D.] Univ Wisconsin, US Geol Survey, Wisconsin Cooperat Wildlife Res Unit, Madison, WI USA. [O'Rourke, Katherine I.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Johnson, Chad J.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. RP Robinson, SJ (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, Madison, WI 53715 USA. EM stacie.j.robinson@gmail.com FU USGS FX We thank the USGS for funding supporting this work. Thanks to Sally Madsen-Bouterse for helpful comments on early drafts of this manuscript. Note that any use of trade, product or firm names is for descriptive purposes and does not imply endorsement by the US Government. NR 86 TC 14 Z9 14 U1 6 U2 60 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 IS 2 BP 153 EP 162 DI 10.4161/pri.19640 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 948SW UT WOS:000304523900010 PM 22460693 ER PT J AU Silva, CJ AF Silva, Christopher J. TI Using small molecule reagents to selectively modify epitopes based on their conformation SO PRION LA English DT Article DE mass spectrometry; prion; antibody; epitope; Me7; 263K; chemical modification; conformation ID TRANSMISSIBLE MINK ENCEPHALOPATHY; SCRAPIE PRION PROTEIN; CHEMICAL CROSS-LINKING; MASS-SPECTROMETRY; PRP 27-30; STRAINS; ANTIBODIES; HAMSTERS; ESTERS; AGENT AB Do not distribute. PrPSc is an infectious protein. The only experimentally verified difference between PrPSc and its normal cellular isoform ( PrPC) is conformational. This work describes an approach to determining the presence of surface exposed or sequestered amino acids present in the PrPSc isoform. The N-hydroxysuccinimide esters of acetic acid and 4-trimethylammoniumbutyric acid were synthesized and reacted with detergent-solubilized brain extracts from Me7-infected mice, uninfected mice, 263K-infected hamsters or uninfected hamsters. These reaction mixtures were analyzed by western blots probed with the antibodies 3F4, 6D11, 7D9, AG4, AH6, GE8 or MAB5424. The 3F4, 6D11, AH6 and GE8 antibodies recognize an epitope that is encrypted in the PrPSc isoform, but exposed in the PrPC isoform. These reagents permit the detection of prion infected brain extracts without the need for proteinase K digestion. In addition they can be used, with an appropriate antibody, to determine which amino acids of PrPSc are exposed on the surface and which are encrypted, thus providing useful structural information. This approach was used to distinguish between the 263K and drowsy strains of hamster-adapted scrapie without the use of proteinase K. C1 USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Silva, CJ (reprint author), USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov OI Silva, Christopher/0000-0003-4521-6377 NR 42 TC 8 Z9 8 U1 0 U2 5 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 IS 2 BP 163 EP 173 DI 10.4161/pri.18795 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 948SW UT WOS:000304523900011 PM 22436143 ER PT J AU Kwon, SJ Truco, MJ Hu, JG AF Kwon, Soon-Jae Truco, Maria-Jose Hu, Jinguo TI LSGermOPA, a custom OPA of 384 EST-derived SNPs for high-throughput lettuce (Lactuca sativa L.) germplasm fingerprinting SO MOLECULAR BREEDING LA English DT Article DE Lettuce; Germplasm; SNP marker; Diversity ID GENETIC-LINKAGE MAP; POPULATION-STRUCTURE; CULTIVATED LETTUCE; MOLECULAR MARKERS; HIGH-DENSITY; DIVERSITY; IDENTIFICATION; POLYMORPHISMS; ORIGIN; GENOME AB To deploy a high-throughput genotyping platform in germplasm management, we designed and tested a custom OPA (Oligo Pool All), LSGermOPA, for assessing the genetic diversity and population structure of the USDA cultivated lettuce (Lactuca sativa L.) germplasm collection using Illumina's GoldenGate assay. This OPA contains 384 EST (expressed sequence tag)-derived SNP (single nucleotide polymorphism) markers selected from a large set of SNP markers experimentally validated and mapped by the Compositae Genome Project. Used for genotyping were DNA samples prepared from bulked leaves of five randomly-selected seedlings from each of 380 lettuce accessions. High-quality genotype data were obtained from 354 of the 384 SNPs. The reproducibility of automatic genotype calls was 99.8% as calculated from the four pairs of duplicated DNA samples in the assay. An unexpectedly high percentage of heterozygous genotypes at the polymorphic loci for most accessions indicated a high level of heterogeneity within accessions. Only 148 homogenous accessions, collectively comprising all five horticultural types, were used in subsequent analyses to demonstrate the usefulness of LSGermOPA. The results of phylogenetic relationship, population structure and genetic differentiation analyses were consistent with previous reports using other marker systems. This suggests that LSGermOPA is capable of revealing sufficient levels of polymorphism among lettuce cultivars and is appropriate for rapid assessment of genetic diversity and population structure in the lettuce germplasm collection. Challenges and strategies for effective genotyping and managing lettuce germplasm are discussed. C1 [Kwon, Soon-Jae; Hu, Jinguo] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. [Truco, Maria-Jose] Univ Calif Davis, Genome Ctr, Davis, CA 95620 USA. RP Hu, JG (reprint author), Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, 59 Johnson Hall, Pullman, WA 99164 USA. EM Jinguo.hu@ars.usda.gov FU USDA-ARS CRIS [5438-21000-026-00D]; Hatch Act funds [W006] FX We greatly appreciate the generosity of Richard Michelmore for allowing us to access and use the unpublished data in selecting SNPs for LSGermOPA, and two anonymous reviewers for helpful and constructive comments on the manuscript. We thank Barbara Hellier, Pavelka, Maria, and Corey Whale for technical assistance in the greenhouse and thank Charles Nicolet and Vanessa Rashbrook for performing the SNP genotyping and helping us in using the GenomeStudio software. This research was supported by USDA-ARS CRIS 5438-21000-026-00D and by the Hatch Act funds to Multistate Research Project W006. NR 49 TC 7 Z9 7 U1 0 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 J9 MOL BREEDING JI Mol. Breed. PD APR PY 2012 VL 29 IS 4 SI SI BP 887 EP 901 DI 10.1007/s11032-011-9623-5 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 950JP UT WOS:000304645700005 ER PT J AU Leivar, P Tepperman, JM Cohn, MM Monte, E Al-Sady, B Erickson, E Quail, PH AF Leivar, Pablo Tepperman, James M. Cohn, Megan M. Monte, Elena Al-Sady, Bassem Erickson, Erika Quail, Peter H. TI Dynamic Antagonism between Phytochromes and PIF Family Basic Helix-Loop-Helix Factors Induces Selective Reciprocal Responses to Light and Shade in a Rapidly Responsive Transcriptional Network in Arabidopsis SO PLANT CELL LA English DT Article ID REGULATED GENE-EXPRESSION; AVOIDANCE-RESPONSE; NEGATIVE REGULATOR; INTERACTING FACTOR-3; SEED-GERMINATION; RED-LIGHT; CHLOROPHYLL BIOSYNTHESIS; CHLOROPLAST DEVELOPMENT; MEDIATED DEGRADATION; SIGNAL-TRANSDUCTION AB Plants respond to shade-modulated light signals via phytochrome (phy)-induced adaptive changes, termed shade avoidance. To examine the roles of Phytochrome-Interacting basic helix-loop-helix Factors, PIF1, 3, 4, and 5, in relaying such signals to the transcriptional network, we compared the shade-responsive transcriptome profiles of wild-type and quadruple pif (pifq) mutants. We identify a subset of genes, enriched in transcription factor-encoding loci, that respond rapidly to shade, in a PIF-dependent manner, and contain promoter G-box motifs, known to bind PIFs. These genes are potential direct targets of phy-PIF signaling that regulate the primary downstream transcriptional circuitry. A second subset of PIF-dependent, early response genes, lacking G-box motifs, are enriched for auxin-responsive loci, and are thus potentially indirect targets of phy-PIF signaling, mediating the rapid cell expansion induced by shade. Comparing deetiolation- and shade-responsive transcriptomes identifies another subset of G-box-containing genes that reciprocally display rapid repression and induction in response to light and shade signals. These data define a core set of transcriptional and hormonal processes that appear to be dynamically poised to react rapidly to light-environment changes via perturbations in the mutually antagonistic actions of the phys and PIFs. Comparing the responsiveness of the pifq and triple pif mutants to light and shade confirms that the PIFs act with overlapping redundancy on seedling morphogenesis and transcriptional regulation but that each PIF contributes differentially to these responses. C1 [Leivar, Pablo; Tepperman, James M.; Cohn, Megan M.; Al-Sady, Bassem; Erickson, Erika; Quail, Peter H.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Leivar, Pablo; Tepperman, James M.; Cohn, Megan M.; Al-Sady, Bassem; Erickson, Erika; Quail, Peter H.] USDA, Ctr Plant Gene Express, Albany, CA 94710 USA. [Leivar, Pablo; Monte, Elena] Univ Barcelona, Univ Autonoma Barcelona, CSIC, Dept Mol Genet,Ctr Res Agr Genom,Inst Recerca & T, Barcelona 08193, Cerdanyola Del, Spain. RP Quail, PH (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM quail@berkeley.edu RI Elena, Monte/I-7221-2015; Leivar, Pablo/D-7808-2017 OI Leivar, Pablo/0000-0003-4878-3684 FU "Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa" of the Generalitat de Catalunya [2009-SGR-206]; Marie Curie International Reintegration Grant [PIRG06-GA-2009-256420]; Spanish "Ministerio de Ciencia e Innovacion" [Marie Curie IRG-046568, BIO2006-09254, BIO2009-07675]; National Institutes of Health [GM-47475]; Department of Energy [DEFG03-87ER13742]; USDA Agricultural Research Service Current Research Information System [5335-21000-027-00D] FX We thank Enamul Huq for providing pif1 pif3 seeds, Matthew Hudson for advice on statistics, the Functional Genomics Laboratory at the University of California at Berkeley for hybridizations of the microarrays, Christine Carle and Tiffany Liu for early technical work on the project, Ana Smith for making media and solutions, and Jaime Martinez-Garcia for helpful comments on the manuscript. This work was supported by the "Comissionat per a Universitats i Recerca del Departament d'Innovacio, Universitats i Empresa" fellowship of the Generalitat de Catalunya (Beatriu de Pinos Program) and Marie Curie International Reintegration Grant PIRG06-GA-2009-256420 to P.L., by grants from Marie Curie IRG-046568, Spanish "Ministerio de Ciencia e Innovacion" BIO2006-09254 and BIO2009-07675, and Generalitat de Catalunya 2009-SGR-206 to E.M., and by National Institutes of Health Grant GM-47475, Department of Energy Grant DEFG03-87ER13742, and USDA Agricultural Research Service Current Research Information System Grant 5335-21000-027-00D to P.H.Q. NR 83 TC 68 Z9 71 U1 2 U2 42 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD APR PY 2012 VL 24 IS 4 BP 1398 EP 1419 DI 10.1105/tpc.112.095711 PG 22 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 949SF UT WOS:000304595500010 PM 22517317 ER PT J AU Mandal, MK Chandra-Shekara, AC Jeong, RD Yu, KS Zhu, SF Chanda, B Navarre, D Kachroo, A Kachroo, P AF Mandal, Mihir Kumar Chandra-Shekara, A. C. Jeong, Rae-Dong Yu, Keshun Zhu, Shifeng Chanda, Bidisha Navarre, Duroy Kachroo, Aardra Kachroo, Pradeep TI Oleic Acid-Dependent Modulation of NITRIC OXIDE ASSOCIATED1 Protein Levels Regulates Nitric Oxide-Mediated Defense Signaling in Arabidopsis SO PLANT CELL LA English DT Article ID NITRATE REDUCTASE-ACTIVITY; TURNIP-CRINKLE-VIRUS; SALICYLIC-ACID; FATTY-ACIDS; DIFFERENTIAL EXPRESSION; BINDING PROTEINS; STOMATAL CLOSURE; JASMONIC-ACID; HIGHER-PLANTS; GENE AB The conserved cellular metabolites nitric oxide (NO) and oleic acid (18: 1) are well-known regulators of disease physiologies in diverse organism. We show that NO production in plants is regulated via 18:1. Reduction in 18: 1 levels, via a genetic mutation in the 18: 1-synthesizing gene SUPPRESSOR OF SA INSENSITIVITY OF npr1-5 (SSI2) or exogenous application of glycerol, induced NO accumulation. Furthermore, both NO application and reduction in 18: 1 induced the expression of similar sets of nuclear genes. The altered defense signaling in the ssi2 mutant was partially restored by a mutation in NITRIC OXIDE ASSOCIATED1 (NOA1) and completely restored by double mutations in NOA1 and either of the nitrate reductases. Biochemical studies showed that 18:1 physically bound NOA1, in turn leading to its degradation in a protease-dependent manner. In concurrence, overexpression of NOA1 did not promote NO-derived defense signaling in wild-type plants unless 18: 1 levels were lowered. Subcellular localization showed that NOA1 and the 18: 1 synthesizing SSI2 proteins were present in close proximity within the nucleoids of chloroplasts. Indeed, pathogen-induced or low-18:1-induced accumulation of NO was primarily detected in the chloroplasts and their nucleoids. Together, these data suggest that 18: 1 levels regulate NO synthesis, and, thereby, NO-mediated signaling, by regulating NOA1 levels. C1 [Mandal, Mihir Kumar; Chandra-Shekara, A. C.; Jeong, Rae-Dong; Yu, Keshun; Zhu, Shifeng; Chanda, Bidisha; Kachroo, Aardra; Kachroo, Pradeep] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Navarre, Duroy] Washington State Univ, USDA, Agr Res Serv, Prosser, WA 99350 USA. RP Kachroo, P (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM pk62@uky.edu FU National Science Foundation [EPS 0236913, MCB 0455318, MCB 0920663, DBI 0521587, 051909, 0749731]; K-IDeA Networks of Biomedical Research Excellence of the National Institutes of Health [P20RR16475] FX We thank Nigel Crawford for noa1 seeds, the ABRC for NIA1, NIA2 Salk lines, and nia1 nia2 double mutant seeds, Zach Adam for clpP and clpC antibodies, Marciel Stadnik for help with NO estimations, Lynnette Dirk for help with NOA1 purification, John Johnson for help with GC, Ludmila Lapchyk for technical help, Amy Crume for managing the plant growth facility, and Daniel Klessig for useful discussions. We thank Barry Pogson and anonymous reviewers for useful suggestions. The lipid analyses were performed at the Kansas Lipidomics Research Center Analytical Laboratory, where instrument acquisition and method development were supported by the National Science Foundation (grant nos. EPS 0236913, MCB 0455318, MCB 0920663, and DBI 0521587), the Kansas Technology Enterprise Corporation, the K-IDeA Networks of Biomedical Research Excellence of the National Institutes of Health (grant no. P20RR16475), and Kansas State University. This work was supported by grants from the National Science Foundation (IOS#051909 and IOS#0749731) to A.K. and P.K. NR 73 TC 51 Z9 54 U1 0 U2 25 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD APR PY 2012 VL 24 IS 4 BP 1654 EP 1674 DI 10.1105/tpc.112.096768 PG 21 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 949SF UT WOS:000304595500027 PM 22492810 ER PT J AU Malik, NSA Perez, JL Patt, JE Zibilske, LM Mangan, RL AF Malik, Nasir S. A. Perez, Jose L. Patt, Joseph E. Zibilske, Larry M. Mangan, Robert L. TI Increased infestation of Asian citrus psyllids on cold treated sour orange seedlings: Its possible relation to biochemical changes in leaves SO JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT LA English DT Article DE Amino nitrogen; ammonia; Asian citrus psyllid; cold stress; polyamines; polyphenols ID CHLOROGENIC ACID; NUTRITIONAL QUALITY; INSECT HERBIVORES; GREENING DISEASE; PLANT-RESPONSES; STRESS; AMMONIA; HYPOTHESIS; PUTRESCINE; INVOLVEMENT AB Cold stressed sour orange (Citrus aurantium L.) seedling attracted significantly more Asian citrus psyllid (ACP)(Diaphorina citri Kuwayama) during 5 and 24 h recovery periods compared to control plants in choice test experiment. Cold stressed plants were held placed at 6 +/- 1 degrees C for 6 days and then transferred to a cage at room temperature (28 +/- 2 degrees C) for the ACP choice test. During these 5 and 24 h recovery periods, levels of free alpha amino nitrogen increased by 27% in 24 h post treated samples. Levels of ammonia, a known insect attractant, also increased throughout the recovery period, and after the 24 h ammonia emission was 170% higher in the cold stressed plants than in the controls. Higher levels of putrescine and spermidine were found during 24 h recovery period in cold treated plants than controls. On the other hand, spermine levels in the cold treated leaves decreased by 25% in the first 5 h but then increased to the same levels as the controls in 24 h post cold treatment samples. A number of polyphenols known to be insect deterrents decreased in cold stressed plants. For example, chlorogenic acid, rutin, homoorientin, caffeic acid, naranginin and eriodictoyl decreased significantly in the cold treated plants during 5 and 24 h recovery period. In general, cold stress that increased ACP infestation of sour orange leaves also increased the levels of biochemical insect attractants and decreased the levels of insect deterrents. C1 [Malik, Nasir S. A.; Perez, Jose L.; Patt, Joseph E.; Zibilske, Larry M.; Mangan, Robert L.] ARS, USDA, Weslaco, TX 78596 USA. RP Malik, NSA (reprint author), ARS, USDA, 2413 E Hwy 83, Weslaco, TX 78596 USA. EM nasir.malik@ars.usda.gov NR 51 TC 3 Z9 3 U1 3 U2 9 PU WFL PUBL PI HELSINKI PA MERI-RASTILANTIE 3 C, HELSINKI, FI-00980, FINLAND SN 1459-0255 J9 J FOOD AGRIC ENVIRON JI J. Food Agric. Environ. PD APR PY 2012 VL 10 IS 2 BP 424 EP 429 PN 1 PG 6 WC Food Science & Technology SC Food Science & Technology GA 946FC UT WOS:000304335900074 ER PT J AU Vazquez-Fernandez, E Pastrana, M Ramos, A Alonso, J Stitz, L Vidal, E Dynin, I Silva, C AF Vazquez-Fernandez, Ester Pastrana, Miguel Ramos, Adriana Alonso, Jana Stitz, Lothar Vidal, Enric Dynin, Irina Silva, Christopher TI Probing the structure of GPI-less PrPSc by limited proteolysis SO PRION LA English DT Meeting Abstract C1 [Vazquez-Fernandez, Ester; Pastrana, Miguel; Ramos, Adriana] Univ Santiago de Compostela, Santiago De Compostela, Spain. [Alonso, Jana] IDIS, Lab Prote, Santiago De Compostela, Spain. [Stitz, Lothar] Friedrich Loeffler Inst, Tubingen, Germany. [Vidal, Enric] Univ Autonoma Barcelona, CRESA, Priocat Lab, E-08193 Barcelona, Spain. [Dynin, Irina; Silva, Christopher] USDA, WRRC, Albany, NY USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 26 EP 27 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300053 ER PT J AU Nicholson, E Vrentas, C Greenlee, J AF Nicholson, Eric Vrentas, Catherine Greenlee, Justin TI Host genotype specified PrPSc stability correlates with incubation time SO PRION LA English DT Meeting Abstract ID PRION PROTEIN; CONFORMATIONAL STABILITY C1 [Nicholson, Eric; Vrentas, Catherine; Greenlee, Justin] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. NR 6 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 39 EP 40 PG 2 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300082 ER PT J AU Greenlee, J Smith, J Nicholson, E AF Greenlee, Justin Smith, Jodi Nicholson, Eric TI A comparison of scrapie and chronic wasting disease in white-tailed deer SO PRION LA English DT Meeting Abstract C1 [Greenlee, Justin; Smith, Jodi; Nicholson, Eric] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 40 EP 40 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300083 ER PT J AU Dassanayake, R Schneider, D Herrmann-Hoesing, L Truscott, T Davis, W O'Rourke, K AF Dassanayake, Rohana Schneider, David Herrmann-Hoesing, Lynn Truscott, Thomas Davis, William O'Rourke, Katherine TI Cell-surface expression of PrPc and the presence of scrapie prions in the blood of goats SO PRION LA English DT Meeting Abstract C1 [Dassanayake, Rohana; Davis, William] Washington State Univ, Pullman, WA 99164 USA. [Schneider, David; Herrmann-Hoesing, Lynn; Truscott, Thomas; O'Rourke, Katherine] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 52 EP 52 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300109 ER PT J AU Detwiler, L Bulaga, L AF Detwiler, Linda Bulaga, Leslie TI Absence of scrapie in a purebred Suffolk flock maintained under a government certification program for 20 years SO PRION LA English DT Meeting Abstract C1 [Detwiler, Linda] Mississippi State Univ, Red Bank, NJ USA. [Bulaga, Leslie] US Anim & Plant Hlth Inspect Serv, Vet Serv, USDA, Robbinsville, NJ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 74 EP 74 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300154 ER PT J AU Silva, C Erickson, M Dynin, I Carter, M AF Silva, Christopher Erickson, Melissa Dynin, Irina Carter, Mark TI Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers-New uses for old antibodies SO PRION LA English DT Meeting Abstract C1 [Silva, Christopher; Erickson, Melissa; Dynin, Irina; Carter, Mark] USDA, Albany, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 90 EP 90 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300185 ER PT J AU Wyckoff, C VerCauteren, K Zabel, M AF Wyckoff, Christy VerCauteren, Kurt Zabel, Mark TI Estimating prion binding capacity of soil SO PRION LA English DT Meeting Abstract C1 [Wyckoff, Christy; Zabel, Mark] Colorado State Univ, Ft Collins, CO 80523 USA. [VerCauteren, Kurt] Wildlife Serv, Natl Wildlife Res Ctr, USDA, Ft Collins, CO USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 J9 PRION JI Prion PD APR-JUN PY 2012 VL 6 SU S BP 137 EP 137 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 944WK UT WOS:000304234300283 ER PT J AU Overturf, K Vallejo, RL Palti, Y Barrows, FT Parsons, JE AF Overturf, Ken Vallejo, Roger L. Palti, Yniv Barrows, Fredrick T. Parsons, James E. TI Microarray analysis of differential utilization of plant-based diets by rainbow trout SO AQUACULTURE INTERNATIONAL LA English DT Article DE Alternative diets; Expression; Metabolic analysis; Microarray; Pathway analysis ID NONSPECIFIC DEFENSE-MECHANISMS; FAMILY GROWTH-RESPONSE; CHRONIC SERUM SICKNESS; FISH-MEAL REPLACEMENT; PANCREATIC BETA-CELLS; SMOOTH-MUSCLE-CELLS; ONCORHYNCHUS-MYKISS; GENE-EXPRESSION; ATLANTIC SALMON; MESSENGER-RNA AB Microarray analysis was conducted using liver samples from two families of rainbow trout that differed in their growth responses when compared between individuals fed a fishmeal or plant protein-based diet. Differential expression relating to dietary utilization between the two families found significant changes in expression of 33 expressed sequence tags (ESTs). Eight of the differentially expressed ESTs had identified mammalian homologs that had been previously researched with identified cellular interactions and functions. Utilizing pathway analysis software to analyze sequences annotated with known mammalian genes, we were able to map gene pathways and process interactions. From this information, we were able to infer that the metabolic changes associated with utilization of plant protein versus fishmeal were associated with differential regulation of genes related to cell oxidative stress, proliferation, growth and survival. Furthermore, we inferred from the changes we observed in immune response gene expression that ingestion of this plant-based diet upregulated the expression of genes involved in immunoregulatory processes. C1 [Overturf, Ken; Barrows, Fredrick T.] ARS, USDA, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. [Vallejo, Roger L.; Palti, Yniv] ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Parsons, James E.] Troutlodge Inc, Sumner, WA USA. RP Overturf, K (reprint author), ARS, USDA, Hagerman Fish Culture Expt Stn, 3059-F Natl Fish Hatchery Rd, Hagerman, ID 83332 USA. EM Ken.Overturf@ars.usda.gov FU USDA-CSREES-SBIR [2005-33610-16078] FX The authors thank Karen Frank and Lindsey Pierce for their technical contributions. This work was supported by the USDA-CSREES-SBIR grant number 2005-33610-16078. The use of this material is for research and informational purposes only. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. The USDA is an equal opportunity employer. NR 87 TC 2 Z9 2 U1 1 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0967-6120 EI 1573-143X J9 AQUACULT INT JI Aquac. Int. PD APR PY 2012 VL 20 IS 2 BP 213 EP 232 DI 10.1007/s10499-011-9490-4 PG 20 WC Fisheries SC Fisheries GA 943EK UT WOS:000304103100002 ER PT J AU Yu, J Johnson, EJ Shang, F Lim, A Zhou, HY Cui, L Xu, J Snellingen, T Liu, XP Wang, NL Liu, NP AF Yu, Jie Johnson, Elizabeth J. Shang, Fu Lim, Apiradee Zhou, Haiying Cui, Lei Xu, Jun Snellingen, Torkel Liu, Xipu Wang, Ningli Liu, Ningpu TI Measurement of Macular Pigment Optical Density in a Healthy Chinese Population Sample SO INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE LA English DT Article ID AGE-RELATED MACULOPATHY; HETEROCHROMATIC FLICKER PHOTOMETRY; RESONANCE RAMAN MEASUREMENT; SPATIAL-DISTRIBUTION; GEOGRAPHIC REGION; EYE DISEASE; VITAMIN-A; CAROTENOIDS; DEGENERATION; PREVALENCE AB PURPOSE. Macular pigment may protect against age-related macular degeneration (AMD) by its capacity to absorb blue light and scavenge free radicals. Current information on human macular pigment density has been largely from studies on Caucasian populations. The purpose of this study was to assess macular pigment density and its determinant factors in a Chinese population sample. METHODS. Macular pigment optical density (MPOD) was measured in a healthy Chinese population using heterochromatic flicker photometry (HFP). Participants received a standard ophthalmic examination, and only subjects who were confirmed not to have any eye diseases except mild age-related cataract were included in the study. Demographic and lifestyle data and general health status were recorded by questionnaire. RESULTS. A total of 281 unrelated healthy Chinese individuals, including 96 males and 185 females, with ages ranging from 17 to 85 years, participated in the study. The mean and standard deviation of MPOD levels were 0.56 +/- 0.19, 0.49 +/- 0.18, 0.36 +/- 0.15, and 0.19 +/- 0.12, respectively, at 0.25 degrees, 0.5 degrees, 1.0 degrees, and 1.75 degrees eccentricity points. A significant age-related decline in MPOD was observed at 0.25 degrees (P = 0.014). Females tended to have relatively lower levels of MPOD than males at 0.25 degrees (P = 0.21), 0.5 degrees (P = 0.025), and 1.0 degrees (P = 0.16). No statistically significant association of MPOD was observed with body mass index or smoking status. CONCLUSIONS. Macular pigment density measured by HFP tended to decline with aging in this healthy Chinese population sample. Females may have lower levels of MPOD than males. (Invest Ophthalmol Vis Sci. 2012;53:2106-2111) DOI:10.1167/iovs.11-8518 C1 [Yu, Jie; Zhou, Haiying; Cui, Lei; Xu, Jun; Wang, Ningli; Liu, Ningpu] Capital Med Univ, Beijing Tongren Eye Ctr, Beijing Tongren Hosp, Beijing Ophthalmol & Visual Sci Key Lab, Beijing 100730, Peoples R China. [Johnson, Elizabeth J.; Shang, Fu] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Lim, Apiradee] Prince Songkla Univ, Fac Sci & Technol, Dept Math & Comp Sci, Muang Pattani, Thailand. [Snellingen, Torkel; Liu, Xipu] Sekwa Eye Hosp, Beijing, Peoples R China. RP Liu, NP (reprint author), Capital Med Univ, Beijing Tongren Eye Ctr, Beijing Tongren Hosp, Beijing Ophthalmol & Visual Sci Key Lab, 1 Dong Jiao Min Xiang, Beijing 100730, Peoples R China. EM nliu001@gmail.com FU National Basic Research Program of China (973 Program) [2007CB512201]; Beijing Municipal Health Bureau [2009208]; National Natural Science Foundation of China [81070734]; Kemin Japan FX Supported in part by the National Basic Research Program of China (973 Program) Grant 2007CB512201, the Beijing Municipal Health Bureau Grant 2009208, the National Natural Science Foundation of China Grant 81070734, and an unrestricted research fund from Kemin Japan. Kemin Health USA kindly provided the heterochromatic flicker photometer for the study. NR 53 TC 11 Z9 12 U1 1 U2 11 PU ASSOC RESEARCH VISION OPHTHALMOLOGY INC PI ROCKVILLE PA 12300 TWINBROOK PARKWAY, ROCKVILLE, MD 20852-1606 USA SN 0146-0404 J9 INVEST OPHTH VIS SCI JI Invest. Ophthalmol. Vis. Sci. PD APR PY 2012 VL 53 IS 4 BP 2106 EP 2111 DI 10.1167/iovs.11-8518 PG 6 WC Ophthalmology SC Ophthalmology GA 937PC UT WOS:000303669400047 PM 22427543 ER PT J AU Fiscus, EL Booker, FL Sadok, W Burkey, KO AF Fiscus, Edwin L. Booker, Fitzgerald L. Sadok, Walid Burkey, Kent O. TI Influence of atmospheric vapour pressure deficit on ozone responses of snap bean (Phaseolus vulgaris L.) genotypes SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Air pollutants; bioindicator; ozone; snap bean; transpiration; vapour pressure deficit ID WATER-USE; CARBON-DIOXIDE; NORWAY SPRUCE; AMBIENT OZONE; HUMIDITY; PLANT; INJURY; EUROPE; GROWTH; FLUX AB Environmental conditions influence plant responses to ozone (O-3), but few studies have evaluated individual factors directly. In this study, the effect of O-3 at high and low atmospheric vapour pressure deficit (VPD) was evaluated in two genotypes of snap bean (Phaseolus vulgaris L.) (R123 and S156) used as O-3 bioindicator plants. Plants were grown in outdoor controlled-environment chambers in charcoal-filtered air containing 0 or 60 nl l(-1) O-3 (12 h average) at two VPDs (1.26 and 1.96 kPa) and sampled for biomass, leaf area, daily water loss, and seed yield. VPD clearly influenced O-3 effects. At low VPD, O-3 reduced biomass, leaf area, and seed yield substantially in both genotypes, while at high VPD, O-3 had no significant effect on these components. In clean air, high VPD reduced biomass and yield by similar fractions in both genotypes compared with low VPD. Data suggest that a stomatal response to VPD per se may be lacking in both genotypes and it is hypothesized that the high VPD resulted in unsustainable transpiration and water deficits that resulted in reduced growth and yield. High VPD- and water-stress-induced stomatal responses may have reduced the O-3 flux into the leaves, which contributed to a higher yield compared to the low VPD treatment in both genotypes. At low VPD, transpiration increased in the O-3 treatment relative to the clean air treatment, suggesting that whole-plant conductance was increased by O-3 exposure. Ozone-related biomass reductions at low VPD were proportionally higher in S156 than in R123, indicating that differential O-3 sensitivity of these bioindicator plants remained evident when environmental conditions were conducive for O-3 effects. Assessments of potential O-3 impacts on vegetation should incorporate interacting factors such as VPD. C1 [Fiscus, Edwin L.; Booker, Fitzgerald L.; Burkey, Kent O.] ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27607 USA. [Fiscus, Edwin L.; Booker, Fitzgerald L.; Burkey, Kent O.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27607 USA. [Sadok, Walid] Univ Florida, Agron Physiol Lab, Gainesville, FL 32611 USA. RP Booker, FL (reprint author), ARS, USDA, Plant Sci Res Unit, 3127 Ligon St, Raleigh, NC 27607 USA. EM fitz.booker@ars.usda.gov RI Sadok, Walid/C-6649-2017 NR 37 TC 6 Z9 7 U1 2 U2 21 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD APR PY 2012 VL 63 IS 7 BP 2557 EP 2564 DI 10.1093/jxb/err443 PG 8 WC Plant Sciences SC Plant Sciences GA 942NU UT WOS:000304052300014 PM 22268148 ER PT J AU Cohen, SD Tarara, JM Gambetta, GA Matthews, MA Kennedy, JA AF Cohen, Seth D. Tarara, Julie M. Gambetta, Greg A. Matthews, Mark A. Kennedy, James A. TI Impact of diurnal temperature variation on grape berry development, proanthocyanidin accumulation, and the expression of flavonoid pathway genes SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Biosynthesis; climate; flavonoids; flavonols; proanthocyanidins; tannins; temperature; Vitis vinifera ID VITIS-VINIFERA L.; CV PINOT-NOIR; R2R3-MYB TRANSCRIPTION FACTOR; CABERNET-SAUVIGNON BERRIES; ANTHOCYANIN BIOSYNTHESIS; LEUCOANTHOCYANIDIN REDUCTASE; PHENYLPROPANOID PATHWAY; CELLULAR-LOCALIZATION; SUNLIGHT EXPOSURE; SOLAR-RADIATION AB Little is known about the impact of temperature on proanthocyanidin (PA) accumulation in grape skins, despite its significance in berry composition and wine quality. Field-grown grapes (cv. Merlot) were cooled during the day or heated at night by +/-8 degrees C, from fruit set to veraison in three seasons, to determine the effect of temperature on PA accumulation. Total PA content per berry varied only in one year, when PA content was highest in heated berries (1.46 mg berry(-1)) and lowest in cooled berries (0.97 mg berry(-1)). In two years, cooling berries resulted in a significant increase in the proportion of (-)-epigallocatechin as an extension subunit. In the third year, rates of berry development, PA accumulation, and the expression levels of several genes involved in flavonoid biosynthesis were assessed. Heating and cooling berries altered the initial rates of PA accumulation, which was correlated strongly with the expression of core genes in the flavonoid pathway. Both heating and cooling altered the rate of berry growth and coloration, and the expression of several structural genes within the flavonoid pathway. C1 [Cohen, Seth D.; Kennedy, James A.] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA. [Tarara, Julie M.] ARS, USDA, Hort Crops Res Unit, Prosser, WA 99350 USA. [Gambetta, Greg A.; Matthews, Mark A.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. RP Cohen, SD (reprint author), Appalachian State Univ, Boone, NC 28608 USA. EM jakennedy@csufresno.edu RI Matthews, Mark/A-5714-2010; Kennedy, James/A-1636-2012 OI Kennedy, James/0000-0002-5453-8469 NR 71 TC 46 Z9 48 U1 6 U2 84 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 J9 J EXP BOT JI J. Exp. Bot. PD APR PY 2012 VL 63 IS 7 BP 2655 EP 2665 DI 10.1093/jxb/err449 PG 11 WC Plant Sciences SC Plant Sciences GA 942NU UT WOS:000304052300022 PM 22268158 ER PT J AU Goerndt, ME Aguilar, FX Miles, P Shifley, S Song, NF Stelzer, H AF Goerndt, Michael E. Aguilar, Francisco X. Miles, Patrick Shifley, Stephen Song, Nianfu Stelzer, Hank TI Regional Assessment of Woody Biomass Physical Availability as an Energy Feedstock for Combined Combustion in the US Northern Region SO JOURNAL OF FORESTRY LA English DT Article DE woody biomass; renewable energy; co-combustion; US Forest Inventory and Analysis ID UNITED-STATES; CARBON; COST AB Woody biomass is a renewable energy feedstock with the potential to reduce current use of nonrenewable fossil fuels. We estimated the physical availability of woody biomass for cocombustion at coal-fired electricity plants in the 20-state US northern region. First, we estimated the total amount of woody biomass needed to replace total annual coal-based electricity consumption at the state level to provide a representation of the potential energy footprints associated with using woody biomass for electric energy. If all woody biomass available were used for electric generation it could replace no more than 19% of coal-based electric generation or 11% of total electric energy generation. Second, we examined annual woody biomass increment at the state level in a series of concentric circles around existing coal-fired electricity plants to examine some of the opportunities and limitations associated with using woody biomass for cofiring at those plants to coincide with state-level renewable portfolio standards. On average, an individual coal-fired power electricity plant could theoretically replace 10% of annual coal use if it obtained 30% of the net annual woody biomass increment within a 34-km radius of the plant. In reality, the irregular spatial distribution of coal-fired power plants means potential biomass supply zones overlap and would greatly diminish opportunities for cofiring with biomass, numerous other regulatory, economic, and social considerations notwithstanding. Given that woody biomass use for electricity will be limited to selected locations, use of woody biomass for energy should be complementary with other forest conservation goals. C1 [Goerndt, Michael E.; Aguilar, Francisco X.; Song, Nianfu; Stelzer, Hank] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Miles, Patrick] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. [Shifley, Stephen] US Forest Serv, No Res Stn, USDA, Columbia, MO 65211 USA. RP Goerndt, ME (reprint author), Univ Missouri, Dept Forestry, 203 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM goerndtm@missouri.edu; aguilarf@missouri.edu; pmiles@fs.fed.us; sshifley@fs.fed.us; songn@missouri.edu; stelzerh@missouri.edu FU Northern Research Station under US Forest Service [09-PA-11242305-143] FX Michael E. Goerndt (goerndtm@missouri.edu) is postdoctoral fellow, and Francisco X. Aguilar (aguilarlf@missouri.edu) is assistant professor, Department of Forestry, University of Missouri, 203 Anheuser-Busch Natural Resources Building, Columbia, MO 65211. Patrick Miles (pmiles@fs.fed.us) is research forester, Northern Research Station, USDA Forest Service, St. Paul MN 55108. Stephen Shifley (sshifley@fs.fed.us) is research forester, Northern Research Station, USDA Forest Service, Columbia, MO 65211. Nianfu Song (songn@missouri.edu) is postdoctoral fellow, Department of Forestry, University of Missouri, 203 Anheuser-Busch Natural Resources Building, Columbia, MO 65211. Hank Stelzer (stelzerh@missouri.edu) is associate professor, Department of Forestry, University of Missouri, 203 Anheuser-Busch Natural Resources Building, Columbia, MO 65211. The authors thank Gregg Coffin, Carl Mize, Gene Garrett, and John Dwyer for their good advice and assistance through the development of this article. This research was supported by the Northern Research Station under US Forest Service Agreement 09-PA-11242305-143. NR 31 TC 14 Z9 14 U1 2 U2 17 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD APR-MAY PY 2012 VL 110 IS 3 BP 138 EP 148 DI 10.5849/jof.10-095 PG 11 WC Forestry SC Forestry GA 941DY UT WOS:000303945100006 ER PT J AU Asah, ST Blahna, DJ Ryan, CM AF Asah, Stanley T. Blahna, Dale J. Ryan, Clare M. TI Involving Forest Communities in Identifying and Constructing Ecosystem Services: Millennium Assessment and Place Specificity SO JOURNAL OF FORESTRY LA English DT Article DE ecosystem services; millennium assessment; social-ecological systems; focus group interviews; Deschutes National Forest ID CLASSIFICATION; SYSTEMS AB The ecosystem services (ES) approach entails integrating people into public forest management and managing to meet their needs and wants. Managers must find ways to understand what these needs are and how they are met. In this study, we used small group discussions, in a case study of the Deschutes National Forest, to involve community members and forest staff in determining what and how people benefit from forests. We compare results with the Millennium Ecosystem Assessment (MA) classification. Results show that people identified benefits in many of the same ways and categories as in the MA. Small group discussants also merged or expanded existing MA categories in novel ways. They identified new benefits not found in the MA classification scheme but identified only four of eight subcategories of regulating services and no supporting services. These findings imply that involving people in the place-specific management of public forests using the ES approach gives managers a clearer understanding of the benefits people recognize and value, as well as those they either are not aware of or do not value. Such information is useful in forest management and in public outreach. C1 [Asah, Stanley T.; Ryan, Clare M.] Univ Washington, Sch Environm & Forest Sci, Coll Environm, Seattle, WA 98195 USA. [Blahna, Dale J.] US Forest Serv, Pacific NW Res Stn, Washington, DC USA. RP Asah, ST (reprint author), Univ Washington, Sch Environm & Forest Sci, Coll Environm, Box 352100, Seattle, WA 98195 USA. EM stasah@u.washington.edu; dblahna@fs.fed.us; cmryan@u.washington.edu OI Ryan, Clare/0000-0003-0986-6110 FU Pacific Northwest Research Station, of the US Forest Service FX Stanley T. Asah (stasah@u.washington.edu) is assistant professor of Human Dimensions of Natural Resources Management, School of Environmental and Forest Sciences, University of Washington, Box 352100, Seattle, WA 98195-2100. Dale J. Blahna (dblahna@fs.fed.us) is research social scientist and team leader, Pacific Northwest Research Station, US Forest Service. Clare M. Ryan (cmryan@u.washington.edu) is professor of Environmental Policy and Conflict Management, School of Environmental and Forest Sciences, College of the Environment, University of Washington, Box 352100, Seattle, WA 98195-2100. The authors thank all those who participated in focus group interviews. They are grateful to John Allen of the Deschutes National Forest and Bob Deal of the Pacific Northwest Research Station for their insights and logistical help through all stages of this study. They thank Cynthia Glick and Tami Kerr for their invaluable help with participant recruitment and for being very welcoming hosts to focus group participants. They extend their gratitude to Ian Bell and David French for assisting with the collection and transcription of data. The authors are especially thankful for the Pacific Northwest Research Station, of the US Forest Service, for funding this study. They appreciate the thorough reviews from three anonymous reviewers and the Associate Editor. NR 19 TC 4 Z9 8 U1 1 U2 15 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD APR-MAY PY 2012 VL 110 IS 3 BP 149 EP 156 DI 10.5849/jof.11-054 PG 8 WC Forestry SC Forestry GA 941DY UT WOS:000303945100007 ER PT J AU Burks, CS Kuenen, LPS AF Burks, Charles S. Kuenen, L. P. S. TI Effect of mating disruption and lure load on the number of Plodia interpunctella (Hubner) (Lepidoptera: Pyralidae) males captured in pheromone traps SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Indianmeal moth; Mating disruption; Monitoring; Pheromone lure ID NAVEL ORANGEWORM LEPIDOPTERA; INDIANMEAL MOTH LEPIDOPTERA; ORIENTAL FRUIT MOTH; MALE CODLING MOTH; EPHESTIA-KUEHNIELLA; SEX-PHEROMONE; SYNTHETIC PHEROMONE; CYDIA-POMONELLA; STORED PRODUCTS; FIELD AB A series of experiments examined the potential for using pheromone lures to monitor Indianmeal moth, Plodia interpunctella (Hubner), in the presence of mating disruption. When males were released in either the presence or absence of commercial mating disruption dispensers, the number of males captured in traps baited with synthetic pheromone lures increased with doses of 1-30 mg per lure (compared to 1-2 mg used in commercial monitoring lures). More males were captured in traps baited with synthetic pheromone lures than in traps baited with females as a pheromone source. The proportion of males captured in traps baited with synthetic pheromone lures vs. traps with females as a pheromone source changed with male age, but not with exposure to mating disruption dispensers. Male interaction with mating disruption dispensers was observed, and suppression of male capture in female-baited traps was quickly lost when mating disruption dispensers were removed. Implications of these findings for monitoring and semiochemical control of P. interpunctella are discussed. Published by Elsevier Ltd. C1 [Burks, Charles S.; Kuenen, L. P. S.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Burks, CS (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM Charles.Burks@ars.usda.gov OI Burks, Charles/0000-0003-0242-8644 NR 39 TC 3 Z9 3 U1 1 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X J9 J STORED PROD RES JI J. Stored Prod. Res. PD APR PY 2012 VL 49 BP 189 EP 195 DI 10.1016/j.jspr.2012.03.001 PG 7 WC Entomology SC Entomology GA 942UX UT WOS:000304076600006 ER PT J AU Morgan, JK Zhou, LJ Li, WB Shatters, RG Keremane, M Duan, YP AF Morgan, J. Kent Zhou, Lijuan Li, Wenbin Shatters, Robert G. Keremane, Manjunath Duan, Yong-Ping TI Improved real-time PCR detection of 'Candidatus Liberibacter asiaticus' from citrus and psyllid hosts by targeting the intragenic tandem-repeats of its prophage genes SO MOLECULAR AND CELLULAR PROBES LA English DT Article DE Liberibacter; Real-time PCR; hyv(I)/hyv(II) genes; Tandem-repeats ID POLYMERASE-CHAIN-REACTION; GREENING DISEASE; XANTHOMONAS-CITRI; DNA; QUANTIFICATION; BACTERIUM; PATHOGEN; AMPLIFICATION; DIAGNOSIS; LEAVES AB 'Candidatus Liberibacter asiaticus' (CLas) is a Gram-negative alpha-proteobacterium, and the prominent species of Liberibacter associated with a devastating worldwide citrus disease known as huanglongbing (HLB). This fastidious bacterium resides in phloem sieve cells of host plants and is vectored by the Asian citrus psyllid (Diaphorina citri). Due to its uneven distribution in planta and highly variable bacterial titers, detection of HLB bacteria can be challenging. Here we demonstrated a new utility of nearly identical tandem-repeats of two CLas prophage genes for real-time PCR by SYBR Green 1 (LJ900fr) and TaqMan(R) (LJ900fpr). When compared with conventional 16S rDNA-based real-time PCR, targeting the repeat sequence reduced the relative detectable threshold by approximately 9 and 3 real-time PCR cycles for LJ900fr and LJ900fpr, respectively. Additionally, both LJ900 methods detected CLas from otherwise non-detectable samples by other methods. CLas was also detected from globally derived samples including psyllids, various citrus varieties, periwinkle, dodder, and orange jasmine, suggesting the new detection method can be applicable worldwide. Additionally, we demonstrated the presence of the hyv(I)/hyv(II) repeat sequence within the 'Ca. Liberibacter americanus' strain. The method thereby provides sensitive HLB detection with broad application for scientific, regulatory, and citrus grower communities. Published by Elsevier Ltd. C1 [Morgan, J. Kent; Shatters, Robert G.; Duan, Yong-Ping] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Zhou, Lijuan] Univ Florida, IFAS IRREC, Ft Pierce, FL 34945 USA. [Li, Wenbin] USDA APHIS PPQ CPHST, Beltsville, MD 20705 USA. [Keremane, Manjunath] USDA ARS, Riverside, CA 92507 USA. RP Duan, YP (reprint author), USDA ARS, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM yongping.duan@ars.usda.gov FU Citrus Advanced Technology Program [162, 310]; FDACS [015579] FX The authors thank Christina T. Latza for her excellent technical support, Lupe Heldoorn and Lutfi Al-Khouja for conducting DNA extractions, Melissa Doud and Lesley Benyon for their critical review of the manuscript. DNA from the Philippines, Thailand and South Africa were kindly provided by Juliet Ochasan, Angsana Akarapisan, and Titus Gerhard respectively. Funding for this work was provided by the Citrus Advanced Technology Program, contract # 162 and 310.; Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture and by the Specialty Crop Block Grant, FDACS contract #015579. NR 32 TC 20 Z9 23 U1 2 U2 25 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0890-8508 J9 MOL CELL PROBE JI Mol. Cell. Probes PD APR PY 2012 VL 26 IS 2 BP 90 EP 98 DI 10.1016/j.mcp.2011.12.001 PG 9 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Cell Biology GA 944UF UT WOS:000304228600005 PM 22245034 ER PT J AU Bobokalonov, JT Kasimova, GF Muhidinov, ZK Jonmurodov, AS Khalikov, DK Liu, LS AF Bobokalonov, J. T. Kasimova, G. F. Muhidinov, Z. K. Jonmurodov, A. S. Khalikov, D. Kh Liu, LinShu TI Kinetics of piroxicam release from low-methylated pectin/zein hydrogel microspheres SO PHARMACEUTICAL CHEMISTRY JOURNAL LA English DT Article DE pectin; zein; microspheres; drug delivery system; release kinetics; piroxicam ID DESIGN AB The kinetics of a model drug (piroxicam) release from pectin/zein hydrogel microspheres was studied under conditions simulating the gastrointestinal tract. It is established that the rate-limiting step in the release mechanism is drug diffusion out of the microspheres rather than its dissolution. The results demonstrate the effectiveness of using this system based on hydrogel microspheres as a sustained drug delivery system capable of prolonging drug release for a period of 36 h and more with allowance for diffusion and enzymatic biodegradation in the hydrogel. C1 [Bobokalonov, J. T.; Kasimova, G. F.; Muhidinov, Z. K.; Jonmurodov, A. S.; Khalikov, D. Kh] Tajik Acad Sci, VI Nikitin Chem Inst, Dushanbe, Tajikistan. [Liu, LinShu] USDA, Eastern Reg Res Ctr, Philadelphia, PA 19118 USA. RP Bobokalonov, JT (reprint author), Tajik Acad Sci, VI Nikitin Chem Inst, Dushanbe, Tajikistan. NR 9 TC 8 Z9 9 U1 0 U2 31 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0091-150X J9 PHARM CHEM J+ JI Pharm. Chem. J. PD APR PY 2012 VL 46 IS 1 BP 50 EP 53 DI 10.1007/s11094-012-0732-1 PG 4 WC Chemistry, Medicinal; Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 944AC UT WOS:000304169300010 ER PT J AU Chacon-Madrigal, E Johnson, MT Hanson, P AF Chacon-Madrigal, Eduardo Johnson, M. Tracy Hanson, Paul TI THE LIFE HISTORY AND IMMATURE STAGES OF THE WEEVIL ANTHONOMUS MONOSTIGMA CHAMPION (COLEOPTERA: CURCULIONIDAE) ON MICONIA CALVESCENS DC (MELASTOMATACEAE) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE biological control; fruit-borer; invasive species; host plants; Costa Rica; Hawaii ID SPECIES GROUP COLEOPTERA; COSTA-RICA; SOLANACEAE; BIOLOGY; HAWAII; AGENT AB We describe and illustrate the life history and immature stages of Anthonomus monostigma Champion (Curculionidae: Curculioninae: Anthonomini). This weevil is a fruit borer in Miconia calvescens DC (Melastomataceae), a Neotropical tree that is invasive in Pacific islands. The larva has three instars, and development from egg to adult requires approximately two months. In Costa Rica, A. monostigma larvae were found in three Miconia species, and adults fed only on Miconia species. Host relationships of the A. monostigma group suggest that this group could be related to the A. partiarius and A. albocivitensis groups (sensu Clark 1992, 1993b). The potential of A. monostigma as a biological control agent is discussed. C1 [Chacon-Madrigal, Eduardo; Hanson, Paul] Univ Costa Rica, Escuela Biol, San Jose, Costa Rica. [Johnson, M. Tracy] USDA Forest Serv, Pacific SW Res Stn, Inst Pacific Isl Forestry, Volcano, HI 96785 USA. RP Chacon-Madrigal, E (reprint author), Univ Costa Rica, Escuela Biol, AP 2060, San Jose, Costa Rica. EM edchacon@gmail.com; tracyjohnson@fs.fed.us; phanson91@gmail.com FU state of Hawaii; National Park Service; USGS Biological Resources Division, via the University of Hawaii Pacific Co-operative Studies Unit; USDA Forest Service FX The authors extend their thanks to Edgar Rojas, Luis Madrigal, Alexander Castillo, Pablo Allen, Manuel Alfaro, Ronald Sanchez and Kenji Nishida for assistance in the field; and to Kenji Nishida, Edgar Rojas and two anonymous reviewers for their comments on the manuscript. We were generously assisted in the identification of Anthonomus species by Robert Anderson. The Proyecto Miconia has been funded by the state of Hawaii, the National Park Service and the USGS Biological Resources Division, via the University of Hawaii Pacific Co-operative Studies Unit, and by USDA Forest Service International Programs. All work was possible thanks to the University of Costa Rica. NR 39 TC 4 Z9 4 U1 0 U2 3 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2012 VL 114 IS 2 BP 173 EP 185 DI 10.4289/0013-8797.114.2.173 PG 13 WC Entomology SC Entomology GA 942DP UT WOS:000304022800001 ER PT J AU Smith, DR Sanchez-Martinez, G Ojeda-Aguilera, A AF Smith, David R. Sanchez-Martinez, Guillermo Ojeda-Aguilera, Amelia TI A NEW SPECIES OF ZADIPRION (HYMENOPTERA: DIPRIONIDAE) ON PINUS DURANGENSIS FROM CHIHUAHUA, MEXICO, AND A REVIEW OF OTHER SPECIES OF THE GENUS SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Review DE falsus; howdeni; rohweri; roteus; townsendi; Guatemala; United States ID SAWFLIES AB Zadipion ojedae Smith and Sanchez-Martinez, n. sp., reared from larvae feeding on Pinus durangensis Martinez in Chihuahua, Mexico, is described, and notes on its life history are provided. Hosts, distributions, and remarks on the other five species of Zadiprion are given, and the females, ovipositors, and male genitalia of all six species are illustrated. An identification key is given for all species. C1 [Smith, David R.] USDA, Systemat Entomol Lab, PSI,Smithsonian Inst, Agr Res Serv,Natl Museum Nat Hist, Washington, DC 20013 USA. [Sanchez-Martinez, Guillermo] Inst Nacl Invest Forestales Agr & Pecuarias, Aguascalientes 20660, Mexico. [Ojeda-Aguilera, Amelia] Secretaria Medio Ambiente & Recursos Nat, Lab Anal & Referencia Sanidad Forestal, Mexico City 04119, DF, Mexico. RP Smith, DR (reprint author), USDA, Systemat Entomol Lab, PSI,Smithsonian Inst, Agr Res Serv,Natl Museum Nat Hist, POB 37012,MRC 168, Washington, DC 20013 USA. EM dave.smith@ars.usda.gov; sanchezm.guillermo@inifap.gob.mx; ojedaamelia@hotmail.com FU Chihuahua State Government FX Hector E. Alanis Morales and Miguel Cano Rodriguez assisted in the biological observations of Zadiprion ojedae. This research was partially funded by the Chihuahua State Government. Henri Goulet, Canadian National Collection of Insects, Ottawa, kindly loaned the holotype of Z. roteus. Michele Touchet (Systematic Entomology Laboratory, USDA, Washington, DC [SEL]) assisted with the images. We appreciate reviews by the following: N. M. Schiff (U.S. Forest Service, Stoneville, MS) and D. A. Nickle and T. J. Henry (SEL, Beltsville, MD, and Washington, DC, respectively). USDA is an equal opportunity provider and employer. NR 20 TC 1 Z9 1 U1 0 U2 1 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2012 VL 114 IS 2 BP 224 EP 237 DI 10.4289/0013-8797.114.2.224 PG 14 WC Entomology SC Entomology GA 942DP UT WOS:000304022800006 ER PT J AU Zhang, S Sherwood, RW Yang, Y Fish, T Chen, W McCardle, JA Jones, RM Yusibov, V May, ER Rose, JKC Thannhauser, TW AF Zhang, Sheng Sherwood, Robert W. Yang, Yong Fish, Tara Chen, Wei McCardle, James A. Jones, R. Mark Yusibov, Vidadi May, Eliel Ruiz Rose, Jocelyn K. C. Thannhauser, Theodore. W. TI Comparative characterization of the glycosylation profiles of an influenza hemagglutinin produced in plant and insect hosts SO PROTEOMICS LA English DT Article DE Glycoproteomics; Influenza hemagglutinin; MRM; Plant recombinant protein; Quantitative glycoforms; Site occupancy ID CHROMATOGRAPHY-MASS-SPECTROMETRY; PROTEIN GLYCOSYLATION; STRUCTURAL CHARACTERIZATION; ABSOLUTE QUANTIFICATION; H5N1 INFLUENZA; OLIGOSACCHARIDES; VIRUS; PHARMACEUTICALS; GLYCOPROTEINS; MIXTURES AB The main objective of this study was to characterize the N-linked glycosylation profiles of recombinant hemagglutinin (HA) proteins expressed in either insect or plant hosts, and to develop a mass spectrometry based workflow that can be used in quality control to assess batch-to-batch reproducibility for recombinant HA glycosylation. HA is a surface glycoprotein of the influenza virus that plays a key role in viral infectivity and pathogenesis. Characterization of the glycans for plant recombinant HA from the viral strain A/California/04/09 (H1N1) has not yet been reported. In this study, N-linked glycosylation patterns of the recombinant HAs from both insect and plant hosts were characterized by precursor ion scan-driven data-dependent analysis followed by high-resolution MS/MS analysis of the deglycosylated tryptic peptides. Five glycosylation sites (N11, N23, N276, N287, and N481) were identified containing high mannose type glycans in plant-expressed HAs, and complex type glycoforms for the insect-expressed HA. More than 95% site occupancy was observed for all glycosylation sites except N11, which was 60% occupied. Multiple-reaction monitoring based quantitation analysis was developed for each glycopeptide isoform and the quantitative results indicate that the Man8GlcNAc2 is the dominant glycan for all sites in plant-expressed HAs. The relative abundance of the glycoforms at each specific glycosylation site and the relative quantitation for each glycoform among three HAs were determined. Few differences in the glycosylation profiles were detected between the two batches of plant HAs studied, but there were significant differences between the glycosylation patterns in the HAs generated in plant and insect expression hosts. C1 [Zhang, Sheng; Sherwood, Robert W.; Chen, Wei; McCardle, James A.] Cornell Univ, Inst Biotechnol & Life Sci Biotechnol, Ithaca, NY 14853 USA. [Yang, Yong; Fish, Tara; Thannhauser, Theodore. W.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Jones, R. Mark; Yusibov, Vidadi] Fraunhofer USA Ctr Mol Biotechnol, Newark, DE USA. [May, Eliel Ruiz; Rose, Jocelyn K. C.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. RP Zhang, S (reprint author), Cornell Univ, Inst Biotechnol & Life Sci Biotechnol, 139 Biotechnol Bldg, Ithaca, NY 14853 USA. EM sz14@cornell.edu FU NIH SIG [1S10RR025449-01]; ARS CRIS [1907-21000-033/034-00D]; NSF [DBI-0606596, DBI-0606595] FX This work was partially supported by NIH SIG grant 1S10RR025449-01, ARS CRIS project numbers 1907-21000-033/034-00D, NSF Plant Genome Grant DBI-0606596 and DBI-0606595. NR 42 TC 18 Z9 18 U1 2 U2 25 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1615-9853 J9 PROTEOMICS JI Proteomics PD APR PY 2012 VL 12 IS 8 BP 1269 EP 1288 DI 10.1002/pmic.201100474 PG 20 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 940UI UT WOS:000303918200021 PM 22577028 ER PT J AU Mitchell, JP Singh, PN Wallender, WW Munk, DS Wroble, JF Horwath, WR Hogan, P Roy, R Hanson, BR AF Mitchell, Jeffrey P. Singh, Purnendu N. Wallender, Wesley W. Munk, Daniel S. Wroble, Jon F. Horwath, William R. Hogan, Philip Roy, Robert Hanson, Blaine R. TI No-tillage and high-residue practices reduce soil water evaporation SO CALIFORNIA AGRICULTURE LA English DT Article ID COTTON AB Reducing tillage and maintaining crop residues on the soil surface could improve the water use efficiency of California crop production. In two field studies comparing no-tillage with standard tillage operations (following wheat silage harvest and before corn seeding), we estimated that 0.89 and 0.97 inches more water was retained in the no-tillage soil than in the tilled soil. In three field studies on residue coverage, we recorded that about 0.56, 0.58 and 0.42 inches more water was retained in residue-covered soil than in bare soil following 6 to 7 days of overhead sprinkler irrigation. Assuming a seasonal crop evapotranspiration demand of 30 inches, coupling no-tillage with practices preserving high residues could reduce summer soil evaporative losses by about 4 inches (13%). However, practical factors, including the need for different equipment and management approaches, will need to be considered before adopting these practices. C1 [Mitchell, Jeffrey P.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Singh, Purnendu N.; Wallender, Wesley W.; Horwath, William R.; Hanson, Blaine R.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA USA. [Roy, Robert] NRCS, USDA, Fresno, CA USA. RP Mitchell, JP (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. OI Singh, Purnendu/0000-0003-1729-7931 FU UC Water Resources Center FX We gratefully acknowledge Bert Garza, Jaime Solorio, Merf Solorio, Nelson Vallejo and Tracy Waltrip for their assistance at the UC West Side Research and Extension Center in Five Points. We also are grateful to the UC Water Resources Center for a research grant that partially supported this research and to Dwayne Beck of Dakota Lakes Research Farm in Pierre, S.D., for fundamental inspiration for this work. NR 28 TC 23 Z9 23 U1 1 U2 26 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 EI 2160-8091 J9 CALIF AGR JI Calif. Agric. PD APR-JUN PY 2012 VL 66 IS 2 BP 55 EP 61 DI 10.3733/ca.v066n02p55 PG 7 WC Agriculture, Multidisciplinary SC Agriculture GA 937GR UT WOS:000303647500009 ER PT J AU Ortega, YK Pearson, DE Waller, LP Sturdevant, NJ Maron, JL AF Ortega, Yvette K. Pearson, Dean E. Waller, Lauren P. Sturdevant, Nancy J. Maron, John L. TI Population-level compensation impedes biological control of an invasive forb and indirect release of a native grass SO ECOLOGY LA English DT Article DE biological control; Centaurea maculosa; Centaurea stoebe; compensation; context dependence; Cyphocleonus achates; exotic plant; herbivory; indirect effects; Pseudoroegneria spicata; spotted knapweed; top-down control ID CYPHOCLEONUS-ACHATES COLEOPTERA; KNAPWEED CENTAUREA-STOEBE; SPOTTED KNAPWEED; WESTERN MONTANA; TROPHIC CASCADE; CONTROL AGENTS; ROOT WEEVIL; PLANT; INSECTS; CURCULIONIDAE AB The intentional introduction of specialist insect herbivores for biological control of exotic weeds provides ideal but understudied systems for evaluating important ecological concepts related to top-down control, plant compensatory responses, indirect effects, and the influence of environmental context on these processes. Centaurea stoebe (spotted knapweed) is a notorious rangeland weed that exhibited regional declines in the early 2000s, attributed to drought by some and to successful biocontrol by others. We initiated an experiment to quantify the effects of the biocontrol agent, Cyphocleonus achates, on Ce. stoebe and its interaction with a dominant native grass competitor, Pseudoroegneria spicata, under contrasting precipitation conditions. Plots containing monocultures of each plant species or equal mixtures of the two received factorial combinations of Cy. achates herbivory (exclusion or addition) and precipitation (May-June drought or "normal,'' defined by the 50-year average) for three years. Cy. achates herbivory reduced survival of adult Ce. stoebe plants by 9% overall, but this effect was stronger under normal precipitation compared to drought conditions, and stronger in mixed-species plots compared to monocultures. Herbivory had no effect on Ce. stoebe per capita seed production or on recruitment of seedlings or juveniles. In normal-precipitation plots of mixed composition, greater adult mortality due to Cy. achates herbivory resulted in increased recruitment of new adult Ce. stoebe. Due to this compensatory response to adult mortality, final Ce. stoebe densities did not differ between herbivory treatments regardless of context. Experimental drought reduced adult Ce. stoebe survival in mixed-species plots but did not impede recruitment of new adults or reduce final Ce. stoebe densities, perhaps due to the limited duration of the treatment. Ce. stoebe strongly depressed P. spicata reproduction and recruitment, but these impacts were not substantively alleviated by herbivory on Ce. stoebe. Population-level compensation by dominant plants may be an important factor inhibiting top-down effects in herbivore-driven and predator-driven cascades. C1 [Ortega, Yvette K.; Pearson, Dean E.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. [Pearson, Dean E.; Waller, Lauren P.; Maron, John L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Sturdevant, Nancy J.] US Forest Serv, USDA, Missoula, MT 59807 USA. RP Ortega, YK (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, 800 E Beckwith Ave, Missoula, MT 59801 USA. EM yortega@fs.fed.us FU USDA Forest Service Forest Health Protection; Rocky Mountain Research Station; Region 1 FX We appreciate K. McKelvey, R. Callaway, and three anonymous reviewers for helpful comments on the manuscript. Many thanks to L. S. Baggett for statistical advice, to W. Good for advice on Cy. achates exclusion, and to J. W. Scarborough, J. Amberson, M. Colburn, C. Henderson, T. McGinley, P. Nicol, S. Sears, and A. Zmud for their hard work in the field. This research was supported by the USDA Forest Service Forest Health Protection, Region 1, and Rocky Mountain Research Station. The Missoula Technology Development Center designed and constructed the drought covers. NR 38 TC 19 Z9 19 U1 3 U2 53 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 J9 ECOLOGY JI Ecology PD APR PY 2012 VL 93 IS 4 BP 783 EP 792 PG 10 WC Ecology SC Environmental Sciences & Ecology GA 937HV UT WOS:000303650500011 PM 22690629 ER PT J AU Jolly, WM Parsons, R Varner, JM Butler, BW Ryan, KC Gucker, CL AF Jolly, W. Matt Parsons, Russell Varner, J. Morgan Butler, Bret W. Ryan, Kevin C. Gucker, Corey L. TI Do mountain pine beetle outbreaks change the probability of active crown fire in lodgepole pine forests? Comment SO ECOLOGY LA English DT Editorial Material ID FUEL BEDS; SPREAD; DECOMPOSITION; SIMULATION C1 [Jolly, W. Matt; Parsons, Russell; Butler, Bret W.; Ryan, Kevin C.; Gucker, Corey L.] US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. [Varner, J. Morgan] Humboldt State Univ, Dept Forestry & Wildland Resources, Arcata, CA 95521 USA. RP Jolly, WM (reprint author), US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. EM mjolly@fs.fed.us NR 27 TC 19 Z9 19 U1 6 U2 14 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 J9 ECOLOGY JI Ecology PD APR PY 2012 VL 93 IS 4 BP 941 EP 946 PG 6 WC Ecology SC Environmental Sciences & Ecology GA 937HV UT WOS:000303650500027 PM 22690645 ER PT J AU Pauli, JN Smith, WP Ben-David, M AF Pauli, Jonathan N. Smith, Winston P. Ben-David, Merav TI Quantifying dispersal rates and distances in North American martens: a test of enriched isotope labeling SO JOURNAL OF MAMMALOGY LA English DT Article DE carbon; deuterium; isotopic enrichment; Martes americana; Martes caurina; mesocarnivore; nitrogen ID STABLE-ISOTOPE; POPULATION-STRUCTURE; CARBON ISOTOPES; HAIR; MIGRATION; HYDROGEN; DIET; N-15; BUTTERFLIES; MUSTELIDAE AB Advances in the application of stable isotopes have allowed the quantitative evaluation of previously cryptic ecological processes. In particular, researchers have utilized the predictable spatial patterning in natural abundance of isotopes to better understand animal dispersal and migration. However, quantifying dispersal via natural abundance alone has proven to be of limited utility for species exhibiting short- or mid-distance dispersal events, including most mammalian species. In previous experimental work, we demonstrated that consumption of 1 dose of isotopically enriched baits elicited a distinct "mark" in hair of captive martens (Martes spp.). Herein, we report findings from a field test of our isotopic enrichment approach to mark free-ranging animals and quantify dispersal of martens across forest stands at sites in southeastern Alaska and northern British Columbia. In the field, we supplemented bait used in single-capture hair traps with the amino acid glycine artificially enriched in H-2, C-13, or N-15. By applying unique combinations of artificially enriched isotopic markers within discrete forest stands, the isotopic signature of collected hair reflected the forest patch where the individual originated. From our isotopic marks, we were able to infer dispersal events between forested stands and, thus, estimate rates and approximate dispersal distances. Our findings demonstrate that isotopic enrichment can be a cost-effective method to mark the hair of midsized mammals for the quantification of dispersal. C1 [Pauli, Jonathan N.; Ben-David, Merav] Univ Wyoming, Program Ecol, Laramie, WY 82071 USA. [Pauli, Jonathan N.; Ben-David, Merav] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Smith, Winston P.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Olympia, WA 98512 USA. RP Pauli, JN (reprint author), Univ Wisconsin, Dept Forest & Wildlife Ecol, 1630 Linden Dr, Madison, WI 53706 USA. EM jnpauli@wisc.edu FU Pacific Northwest Research Station (United States Department of Agriculture Forest Service); National Science Foundation [0808177]; University of Wyoming; American Society of Mammalogists FX We thank R. J. Artery, C. Reynolds, J. Pokallus, G. Campbell, B. Neiles, J. Thomas, and E. Flaherty for assisting with fieldwork and sample processing. R. O. Hall and C. Martinez del Rio provided valuable discussions on calculation of appropriate dosages. Financial support was provided by Pacific Northwest Research Station (United States Department of Agriculture Forest Service), a National Science Foundation Doctoral Dissertation Improvement Grant (0808177), a scholarship through the Program in Ecology at the University of Wyoming, and a fellowship from the American Society of Mammalogists. NR 55 TC 3 Z9 3 U1 3 U2 56 PU ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-2372 EI 1545-1542 J9 J MAMMAL JI J. Mammal. PD APR PY 2012 VL 93 IS 2 BP 390 EP 398 DI 10.1644/11-MAMM-S-163.1 PG 9 WC Zoology SC Zoology GA 937VI UT WOS:000303690700009 ER PT J AU Innes, RJ McEachern, MB Van Vuren, DH Eadie, JM Kelt, DA Johnson, ML AF Innes, Robin J. McEachern, Mary Brooke Van Vuren, Dirk H. Eadie, John M. Kelt, Douglas A. Johnson, Michael L. TI Genetic relatedness and spatial associations of dusky-footed woodrats (Neotoma fuscipes) SO JOURNAL OF MAMMALOGY LA English DT Article DE dusky-footed woodrat; kinship; Neotoma fuscipes; philopatry; Sierra Nevada; space sharing ID MAXIMUM-LIKELIHOOD-ESTIMATION; MICROSATELLITE LOCI; SPACE USE; CLETHRIONOMYS-RUFOCANUS; NULL ALLELES; PHILOPATRY; POPULATION; ORGANIZATION; DISPERSAL; EVOLUTION AB We studied the association between space sharing and kinship in a solitary rodent, the dusky-footed woodrat (Neotoma fuscipes). Genetic relatedness was inversely correlated with geographic distance for female woodrats but not for males, a pattern consistent with female philopatry and male dispersal. However, some female neighbors were unrelated, suggesting the possibility of female dispersal. Relatedness of female dyads was positively correlated with overlap of their home ranges and core areas, indicating that females were more likely to share space with relatives, whereas males showed no correlation between relatedness and the sharing of either home ranges or core areas. However, some females that shared space were not close relatives, and some closely related males shared space. House sharing was exhibited both by close relatives and by distantly related or unrelated woodrats, and was not correlated with relatedness. The kin structuring we describe likely resulted from a pattern of female philopatry and male dispersal, but also may have resulted from kin-directed behaviors by females. C1 [Innes, Robin J.; McEachern, Mary Brooke; Van Vuren, Dirk H.; Eadie, John M.; Kelt, Douglas A.] Univ Calif Davis, Dept Wildlife Fish & Conservat Biol, Davis, CA 95616 USA. [Johnson, Michael L.] Univ Calif Davis, John Muir Inst Environm, Aquat Ecosyst Anal Lab, Davis, CA 95616 USA. RP Innes, RJ (reprint author), US Forest Serv, USDA, Rocky Mt Res Ctr, Fire Sci Lab, 5775 US Highway 10 W, Missoula, MT 59808 USA. EM rjinnes@yahoo.com RI Eadie, John/E-4820-2011 FU Joint Fire Sciences Program; United States Department of Agriculture, Forest Service (Rocky Mountain Research Station and Region 5); University of California Davis FX We thank the dedicated field crews of 2003-2007. We also thank S. Bigelow, J. Keane, P. Shaklee, and P. Stine of the United States Department of Agriculture, Forest Service, Pacific Southwest Research Station, Sierra Nevada Research Center, Davis, California, and G. Rotta, S. Cosmaker, and K. Felker of the United States Department of Agriculture, Forest Service, Mt. Hough Ranger District, Quincy, California, for providing logistical support in the field. We gratefully acknowledge the members of the Quincy Library Group, Quincy, California. This work was supported by the Joint Fire Sciences Program, the United States Department of Agriculture, Forest Service (Rocky Mountain Research Station and Region 5), and the University of California Davis. NR 47 TC 4 Z9 6 U1 2 U2 34 PU ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-2372 J9 J MAMMAL JI J. Mammal. PD APR PY 2012 VL 93 IS 2 BP 439 EP 446 DI 10.1644/11-MAMM-A-171.1 PG 8 WC Zoology SC Zoology GA 937VI UT WOS:000303690700014 ER PT J AU Yao, SR Ludy, JJ Hummer, KE AF Yao, Shengrui Ludy, James J. Hummer, Kim E. TI Cold Hardiness and Foliar Disease Resistance of Northern American and Asian Fragaria SO JOURNAL OF THE AMERICAN POMOLOGICAL SOCIETY LA English DT Article ID PHOTOPERIOD SENSITIVITY; FEMALE FERTILITY; FRUIT SIZE; STRAWBERRY; INHERITANCE; CHILOENSIS; VIRGINIANA; INJURY AB Thirty-two wild strawberry genotypes and two commercial cultivars were obtained from the US Department of Agriculture (USDA), Agricultural Research Service (ARS), National Clonal Germplasm Repository-Corvallis (NCGR) and planted in the field to test cold hardiness and foliar disease resistance at the University of Minnesota North Central Research and Outreach Center at Grand Rapids, MN. This station is located in USDA plant hardiness zone 31). Among the 34 genotypes tested, Fragaria iinumae was sensitive to powdery mildew, leaf scorch, frost and mid-winter injury while F. nipponica was resistant to powdery mildew and leaf scorch. Fragaria iinumae suffered severe injury after winter 2009-10, while F nipponica and F orientalis survived that winter well, producing dense and vigorous plants in 2010. These species could be potentially useful diploid or tetraploid parents in breeding programs developing strawberries for the mid-western United States. Fragaria chiloensis PI 637983, was similar to the standard cultivars for winter hardiness and disease resistance. Wild strawberries bloomed earlier and had softer, smaller fruit than did the commercial F. xananassa 'Jewel' and 'Mesabi'. Fragaria nipponica had the softest fruit. Fragaria orientalis PI 637933 and PI 637939 were notable for their pleasing taste and aroma, while most Fragaria nipponica, F. iturupensis, and F. virginiana were very sour. The feral F. xananassa PI 641087 and PI 641088 may have the greatest immediate utility, with a combination of winter hardiness and excellent disease resistance, for octoploid breeding programs. C1 [Yao, Shengrui; Ludy, James J.] Univ Minnesota, Dept Hort Sci, St Paul, MN 55108 USA. [Hummer, Kim E.] ARS, USDA, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Yao, SR (reprint author), New Mexico State Univ, Sustainable Agr Sci Ctr, Alcalde, NM 87511 USA. EM lubyx001@umn.edu FU USDA ARS [53-5358-8-219]; CRIS [5358-21000-038-00D] FX We would like to acknowledge a grant 53-5358-8-219 from the USDA ARS for germplasm evaluation of small fruits in cooperation with CRIS 5358-21000-038-00D. We thank Keith Mann and Pat Johnson for their technical support at University of Minnesota North Central Research and Outreach Center. NR 17 TC 1 Z9 1 U1 1 U2 13 PU AMER POMOLOGICAL SOC PI UNIVERSITY PK PA 102 TYSON BUILDING, UNIVERSITY PK, PA 16802 USA SN 1527-3741 J9 J AM POMOL SOC JI J. Amer. Pomolog. Soc. PD APR PY 2012 VL 66 IS 2 BP 46 EP 55 PG 10 WC Agronomy; Horticulture SC Agriculture GA 942CI UT WOS:000304019300001 ER PT J AU Engeman, R Martin, RE Woolard, J Stahl, M Pelizza, C Duffiney, A Constantin, B AF Engeman, Richard Martin, R. Erik Woolard, John Stahl, Margo Pelizza, Charles Duffiney, Anthony Constantin, Bernice TI An ideal combination for marine turtle conservation: exceptional nesting season, with low nest predation resulting from effective low-cost predator management SO ORYX LA English DT Article DE Armadillo; bioeconomics; endangered species; Florida; green turtle; leatherback turtle; loggerhead turtle; raccoon ID FISHERIES BYCATCH; POPULATION; RACCOON; DENSITY; FLORIDA; PARK AB We examined impacts from effective predator management on nesting success of marine turtles in an exceptional nesting year at Hobe Sound National Wildlife Refuge, Florida, USA, a beach with a high density of nesting marine turtles that has a history of severe nest predation. Historically up to 95% of nests were predated, primarily by raccoons Procyon lotor and, more recently, armadillos Dasypus novemcinctus. Predator control was identified as the most important conservation tool for marine turtle reproduction. Predator management by refuge staff as ancillary duties typically only held predation levels to c. 50%. However, when experts in predator control were employed predation was substantially reduced. An extraordinary opportunity to evaluate the biological and economic benefits of this management approach occurred in 2008, a year with exceptionally heavy nesting. Loggerhead turtle Caretta caretta nesting resurged, green Chelonia mydas and leatherback Dermochelys coriacea turtles nested in record numbers, producing twice or more than their median number of nests, and the first Kemp's ridley Lepidochelys kempii nest was observed. Overall predation was 14.7%, resulting in an estimated >128,000 additional hatchlings emerging compared to estimates had no predator management been in place and historical predation rates occurred, and >56,000 hatchlings more than expected had predator management been conducted as ancillary duties rather than by experts. The USD 12,000 investment for expert predator management equated to only USD 0.09 spent for each additional hatchling produced compared to the scenario of no predator control and only USD 0.21 compared to the scenario of predator control as ancillary duties. C1 [Engeman, Richard] Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Martin, R. Erik] Ecol Associates Inc, Jensen Beach, FL USA. [Woolard, John; Duffiney, Anthony; Constantin, Bernice] USDA APHIS Wildlife Serv, Gainesville, FL USA. [Stahl, Margo; Pelizza, Charles] Hobe Sound Natl Wildlife Refuge, Hobe Sound, FL USA. RP Engeman, R (reprint author), Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM richard.m.engeman@aphis.usda.gov FU Town of Jupiter Island FX We thank the Town of Jupiter Island for the funding that permitted the predator management to take place and thereby allow a historic number of hatchling marine turtles to be produced. Justin Fischer kindly created Fig. 1. NR 41 TC 1 Z9 1 U1 3 U2 37 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0030-6053 J9 ORYX JI Oryx PD APR PY 2012 VL 46 IS 2 BP 229 EP 235 DI 10.1017/S0030605311000020 PG 7 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 939TG UT WOS:000303837100017 ER PT J AU Belflower, JB Bernard, JK Gattie, DK Hancock, DW Risse, LM Rotz, CA AF Belflower, Jeff B. Bernard, John K. Gattie, David K. Hancock, Dennis W. Risse, Lawrence M. Rotz, C. Alan TI A case study of the potential environmental impacts of different dairy production systems in Georgia SO AGRICULTURAL SYSTEMS LA English DT Article DE Farm simulation; Dairy; Pasture; Carbon footprint; Greenhouse gas; Environment ID GAS REDUCTION STRATEGIES; CARBON FOOTPRINT; MILK-PRODUCTION; NEW-ZEALAND; EMISSIONS; FARMS; SIMULATION; MITIGATION AB The biological and physical processes of an intensively-managed rotational pasture-based dairy and a confinement fed dairy in the southeastern United States were simulated with the Integrated Farm System Model (IFSM) to evaluate management effects on greenhouse gas emissions, soil carbon sequestration, carbon footprint, nitrate leaching, ammonia volatilization, erosion, phosphorus runoff, and phosphorus accumulation in the soil. Edge-of-field erosion and phosphorus runoff were less for the pasture-based dairy per unit of land and per unit of milk produced, but nitrate leaching was greater. Ammonia emissions were greater from the confinement dairy because of the greater handling of manure. Greenhouse gas emissions per cow were greater on the confined dairy, but with greater milk production per cow, the carbon footprint of milk produced was similar to that of the pasture-based dairy. Considering the potential soil carbon sequestration following the conversion of crop land to perennial grassland, the carbon footprint of the milk produced by the pasture-based dairy was slightly less than that of the confinement dairy. The results of this study were generally consistent with similar simulation studies done in the northeastern US with variations due to regional differences in climate, soil type, and agronomic practices. Simulated changes in production practices predicted that increasing milk production through improved animal management or feeding more corn decreased the carbon footprint of milk produced by the pasture-based dairy, while decreasing the inorganic nitrogen fertilizer application rate or raising replacement heifers on the farm had little effect. On the confinement dairy, covering the manure storage and flaring the biogas decreased the carbon footprint, using higher producing, pure-bred Holstein cows or producing less forage on the farm increased the footprint, and eliminating free-stall barns and placing all cattle on pasture had little effect on the footprint. The IFSM was capable of adapting to the climate and production practices of the southeastern US, but further improvements could be made to better represent the cropping practices used in this region. Published by Elsevier Ltd. C1 [Rotz, C. Alan] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Belflower, Jeff B.; Gattie, David K.; Risse, Lawrence M.] Univ Georgia, Driftmier Engn Ctr, Athens, GA 30602 USA. [Bernard, John K.] Univ Georgia, Tifton, GA 31793 USA. RP Rotz, CA (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. EM al.rotz@ars.usda.gov NR 28 TC 21 Z9 22 U1 1 U2 66 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-521X J9 AGR SYST JI Agric. Syst. PD APR PY 2012 VL 108 BP 84 EP 93 DI 10.1016/j.agsy.2012.01.005 PG 10 WC Agriculture, Multidisciplinary SC Agriculture GA 933MJ UT WOS:000303365500009 ER PT J AU Darwish, AM Mitchell, A Straus, DL AF Darwish, Ahmed M. Mitchell, Andrew Straus, David L. TI Evaluation of a 4-h static copper sulphate treatment against experimental infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus) SO AQUACULTURE RESEARCH LA English DT Article DE copper sulphate; columnaris; Flavobacterium columnare; channel catfish; Ictalurus puntatus ID POTASSIUM-PERMANGANATE; EDWARDSIELLA-ICTALURI; RAFINESQUE; SUSCEPTIBILITY; EXPOSURE; EFFICACY; CARP AB The efficacy of copper sulphate (CuSO4) against the early stages of an experimental acute infection of Flavobacterium columnare in channel catfish (Ictalurus punctatus) was evaluated. Fish were challenged, by waterborne exposure to F. columnare in an ultra-low flow-through water delivery system, and treated with CuSO4 at 4.2 and 2.1 mg L-1 at 5.5 h post challenge for 4 h. Bacterial challenged non-treated fish acted as a positive control and non-challenged non-treated fish acted as a negative control. Fish challenged with F. columnare and treated with CuSO4 at 4.2 and 2.1 mg L-1 post challenge had mortalities of 6.4% and 18.3%, respectively, compared with the positive control, which had 90.2% mortality. The mortality of challenged fish treated with CuSO4 at 4.2 and 2.1 mg L-1 was significantly different from the positive control. There was no significant difference between the mortalities of the 4.2 and 2.1 mg L-1 treatments. The results suggest that CuSO4 has clear therapeutic value against columnaris infections. C1 [Darwish, Ahmed M.; Mitchell, Andrew; Straus, David L.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Darwish, AM (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. EM ahmed.darwish@ars.usda.gov NR 36 TC 6 Z9 7 U1 0 U2 2 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1355-557X J9 AQUAC RES JI Aquac. Res. PD APR PY 2012 VL 43 IS 5 BP 688 EP 695 DI 10.1111/j.1365-2109.2011.02876.x PG 8 WC Fisheries SC Fisheries GA 923EZ UT WOS:000302603500006 ER PT J AU Morales, CA Oakley, BB Garrish, JK Siragusa, GR Ard, MB Seal, BS AF Morales, Cesar A. Oakley, Brian B. Garrish, Johnna K. Siragusa, Gregory R. Ard, Mary B. Seal, Bruce S. TI Complete genome sequence of the podoviral bacteriophage Phi CP24R, which is virulent for Clostridium perfringens SO ARCHIVES OF VIROLOGY LA English DT Article ID FAMILY AB Bacteriophage Phi CP24R was isolated from raw sewage from a waste treatment plant, and lytic activity was observed against a type A Clostridium perfringens isolate. Electron microscopy revealed a small virion (44-nm-diameter icosahedral capsid) with a short, non-contractile tail, indicative of a member of the family Podoviridae. The phage had a linear, double-stranded DNA genome of 18,919 base pairs (bp) with 41 bp inverted terminal repeats and a type B DNA polymerase, which are characteristics of members of the subfamily Picovirinae. Out of 22 predicted genes in the genome, ten had significant sequence similarity to proteins of known function. Three distinct genes with lytic domains were identified, including a zinc carboxypeptidase domain that has not been previously reported in viruses. The Phi CP24R genome described herein is only the second Clostridium perfringens podovirus genome reported to date. C1 [Morales, Cesar A.; Oakley, Brian B.; Garrish, Johnna K.; Seal, Bruce S.] ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. [Siragusa, Gregory R.] Danisco USA, Waukesha, WI 53186 USA. [Ard, Mary B.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. RP Morales, CA (reprint author), ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM Cesar.Morales@ars.usda.gov FU ARS-USDA [6612-3200-060-00D] FX This work was supported by ARS-USDA project number 6612-3200-060-00D. We thank Manju Amin, Susan Brooks, and Susan Mize for their technical assistance on this project. NR 22 TC 7 Z9 7 U1 0 U2 10 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PD APR PY 2012 VL 157 IS 4 BP 769 EP 772 DI 10.1007/s00705-011-1218-2 PG 4 WC Virology SC Virology GA 934UT UT WOS:000303472900020 PM 22218967 ER PT J AU Sackett, LC Cross, TB Jones, RT Johnson, WC Ballare, K Ray, C Collinge, SK Martin, AP AF Sackett, Loren C. Cross, Todd B. Jones, Ryan T. Johnson, Whitney C. Ballare, Kimberly Ray, Chris Collinge, Sharon K. Martin, Andrew P. TI Connectivity of prairie dog colonies in an altered landscape: inferences from analysis of microsatellite DNA variation SO CONSERVATION GENETICS LA English DT Article DE Prairie dogs; Microsatellites; Connectivity; Fragmentation; Landscape; Urbanization ID POPULATION GENETIC-STRUCTURE; CYNOMYS-LUDOVICIANUS; HABITAT FRAGMENTATION; ASSIGNMENT METHODS; OROPSYLLA-HIRSUTA; MIGRATION RATE; PLAGUE; FLOW; METAPOPULATION; CONSERVATION AB Connectivity of populations influences the degree to which species maintain genetic diversity and persist despite local extinctions. Natural landscape features are known to influence connectivity, but global anthropogenic landscape change underscores the importance of quantifying how human-modified landscapes disrupt connectivity of natural populations. Grasslands of western North America have experienced extensive habitat alteration, fragmenting populations of species such as black-tailed prairie dogs (Cynomys ludovicianus). Population sizes and the geographic range of prairie dogs have been declining for over a century due to habitat loss, disease, and eradication efforts. In many places, prairie dogs have persisted in the face of emerging urban landscapes that carve habitat into smaller and smaller fragments separated by uninhabitable areas. In extreme cases, prairie dog colonies are completely bounded by urbanization. Connectivity is particularly important for prairie dogs because colonies suffer high probabilities of extirpation by plague, and dispersal permits recolonization. Here we explore connectivity of prairie dog populations using analyses of 11 microsatellite loci for 9 prairie dog colonies spanning the fragmented landscape of Boulder County, Colorado. Isolation-by-resistance modeling suggests that wetlands and high intensity urbanization limit movement of prairie dogs. However, prairie dogs appear to move moderately well through low intensity development (including roads) and freely through cropland and grassland. Additionally, there is a marked decline in gene flow between colonies with increasing geographic distance, indicating isolation by distance even in an altered landscape. Our results suggest that prairie dog colonies retain some connectivity despite fragmentation by urbanization and agricultural development. C1 [Sackett, Loren C.; Jones, Ryan T.; Ballare, Kimberly; Ray, Chris; Collinge, Sharon K.; Martin, Andrew P.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Cross, Todd B.] USDA Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Collinge, Sharon K.] Univ Colorado, Environm Studies Program, Boulder, CO 80309 USA. RP Sackett, LC (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. EM loren.sackett@colorado.edu OI RAY, CHRIS/0000-0002-7963-9637 FU National Center for Environmental Research of the US-Environmental Protection Agency [R-82909101-0]; National Science Foundation/National Institutes of Health [DEB-0224328] FX We thank the City of Boulder Open Space and Mountain Parks Department, the City of Boulder Parks and Recreation Department, the Boulder County Parks and Open Space Department and the Jefferson County Open Space Department for access to study prairie dog colonies on their properties and the Colorado Division of Wildlife for permission to capture and release prairie dogs. Our sincere thanks to the many field workers who collected tissue samples from prairie dogs, especially Amelia Markeson and David Conlin; to Kimberly Kosmenko for collating records of prairie dog relocation; and to Jory Brinkerhoff and Ken Gage for discussion and insight on plague ecology. This research was supported by grants from the National Center for Environmental Research Science to Achieve Results program of the US-Environmental Protection Agency (R-82909101-0) and the National Science Foundation/National Institutes of Health joint program in Ecology of Infectious Diseases (DEB-0224328). NR 48 TC 10 Z9 10 U1 4 U2 82 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 J9 CONSERV GENET JI Conserv. Genet. PD APR PY 2012 VL 13 IS 2 BP 407 EP 418 DI 10.1007/s10592-011-0293-y PG 12 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA 934WD UT WOS:000303476600011 ER PT J AU Potter, KM Jetton, RM Dvorak, WS Hipkins, VD Rhea, R Whittier, WA AF Potter, Kevin M. Jetton, Robert M. Dvorak, William S. Hipkins, Valerie D. Rhea, Rusty Whittier, W. Andrew TI Widespread inbreeding and unexpected geographic patterns of genetic variation in eastern hemlock (Tsuga canadensis), an imperiled North American conifer SO CONSERVATION GENETICS LA English DT Article DE Phylogeography; Pleistocene; Migration; Population genetics; Microsatellite; Bottleneck ID POSTGLACIAL COLONIZATION ROUTES; QUATERNARY VEGETATION HISTORY; CHLOROPLAST DNA POLYMORPHISMS; RECENT POPULATION BOTTLENECKS; WOOLLY ADELGID INFESTATIONS; SOUTHEASTERN UNITED-STATES; HETEROPHYLLA RAF. SARG; ALLELE FREQUENCY DATA; SOUTHERN NEW-ENGLAND; MICROSATELLITE MARKERS AB Eastern hemlock (Tsuga canadensis [L.] Carr.) is an ecologically important tree species experiencing severe mortality across much of its eastern North American distribution, caused by infestation of the exotic hemlock woolly adelgid (Adelges tsugae Annand). To guide gene conservation strategies for this imperiled conifer, we conducted a range-wide genetic variation study for eastern hemlock, amplifying 13 highly polymorphic nuclear microsatellite loci in 1,180 trees across 60 populations. The results demonstrate that eastern hemlock exhibits moderate inbreeding, possibly a signature of a prehistoric decline associated with a widespread insect outbreak. Contrary to expectations, populations in formerly glaciated regions are not less genetically diverse than in the putative southern refugial region. As expected, peripheral disjunct populations are less genetically diverse than main-range populations, but some are highly genetically differentiated or contain unique alleles. Spatially explicit Bayesian clustering analyses suggest that three or four Pleistocene glacial refuges may have existed in the Southeastern United States, with a main post-glacial movement into the Northeast and the Great Lakes region. Efforts to conserve eastern hemlock genetic material should emphasize the capture of broad adaptability that occurs across the geographic range of the species, as well as genetic variability within regions with the highest allelic richness and heterozygosity, such as the Southern Appalachians and New England, and within disjunct populations that are genetically distinct. Much genetic variation exists in areas both infested and uninfested by the adelgid. C1 [Potter, Kevin M.] N Carolina State Univ, Dept Forestry & Environm Resources, Res Triangle Pk, NC 27709 USA. [Jetton, Robert M.; Dvorak, William S.; Whittier, W. Andrew] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Hipkins, Valerie D.] USDA Forest Serv, Natl Forest Genet Lab, Placerville, CA 95667 USA. [Rhea, Rusty] USDA Forest Serv, Asheville, NC 28804 USA. RP Potter, KM (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, 3041 Cornwallis Rd, Res Triangle Pk, NC 27709 USA. EM kpotter@ncsu.edu; rmjetton@ncsu.edu; dvorak@ncsu.edu; vhipkins@fs.fed.us; rrhea@fs.fed.us; andrew_whittier@ncsu.edu OI Potter, Kevin/0000-0002-7330-5345 FU U.S. Department of Agriculture, Forest Service, Forest Health Protection [05-DG-11083150-210, 08-DG-11083150-014, 06-PA-11083150-002]; Camcore, North Carolina State University [05-DG-11083150-210, 08-DG-11083150-014, 06-PA-11083150-002]; U.S. Department of Agriculture, Forest Service, Southern Research Station [05-CA-11330126-210, 08-JV-11330146-078, 10-JV-11330146049]; North Carolina State University [05-CA-11330126-210, 08-JV-11330146-078, 10-JV-11330146049] FX We thank Sedley Josserand for her advice in optimizing microsatellite PCRs; Joe Nairn and Brian Shamblin for providing eastern hemlock microsatellite primer sequences; John Frampton and Dana Nelson for assistance in initiating this project; Ricardo Hernandez and Jennifer DeWoody for laboratory assistance; and Craig Echt, Alistair Jump and two anonymous reviewers for thoughtful comments on the manuscript. We are very grateful to a long list of government organizations listed in Table 1 and their natural resource professionals who assisted with the identification and collection of hemlock samples. This work was supported by in part through Grant Agreements 05-DG-11083150-210 and 08-DG-11083150-014 and Participating Agreement 06-PA-11083150-002 between the U.S. Department of Agriculture, Forest Service, Forest Health Protection and Camcore, North Carolina State University; and through Cooperative Agreement 05-CA-11330126-210 and Research Joint Venture Agreements 08-JV-11330146-078 and 10-JV-11330146049 between the U.S. Department of Agriculture, Forest Service, Southern Research Station, and North Carolina State University. NR 143 TC 12 Z9 12 U1 2 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 J9 CONSERV GENET JI Conserv. Genet. PD APR PY 2012 VL 13 IS 2 BP 475 EP 498 DI 10.1007/s10592-011-0301-2 PG 24 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA 934WD UT WOS:000303476600017 ER PT J AU Snyder, SA Stockmann, KD Morris, GE AF Snyder, Stephanie A. Stockmann, Keith D. Morris, Gaylord E. TI An Optimization Modeling Approach to Awarding Large Fire Support Wildfire Helicopter Contracts from the US Forest Service SO FOREST SCIENCE LA English DT Article DE wildfire resource allocation; goal programming; assignment model; government contracting ID WILDLAND-URBAN INTERFACE; PROGRAMMING-MODEL; INITIAL-ATTACK; SUPPRESSION; ALLOCATION AB The US Forest Service used contracted helicopter services as part of its wildfire suppression strategy. An optimization decision-modeling system was developed to assist in the contract selection process. Three contract award selection criteria were considered: cost per pound of delivered water, total contract cost, and quality ratings of the aircraft and vendors. Discrete optimization decision models were developed and solved to optimize each of these objectives or contract selection criteria independently. These solutions provided the basis for a weighted goal programming model that minimized the percent deviation from each of the three, incorporating relative preference or priority weights on deviations from the goals. Managers chose to assign a greater relative importance to the quality rating than the two cost measures, resulting in a solution with higher quality aircraft, but at a cost of $55 million more than if total cost had been the only contract selection criteria. These optimization models show promise as a means of supporting agency decisionmaking regarding firefighting helicopter contracts. FOR. SCI. 58(2):130-138. C1 [Snyder, Stephanie A.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP Snyder, SA (reprint author), US Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM stephaniesnyder@fs.fed.us; kstockmann@fs.fed.us; emorris02@fs.fed.us FU US Forest Service Northern Research Station; US Forest Service Northern Region FX This research was supported by the US Forest Service Northern Research Station and the US Forest Service Northern Region. We are grateful to Robert Haight for helpful comments on an earlier version of this manuscript. NR 27 TC 0 Z9 0 U1 2 U2 8 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD APR PY 2012 VL 58 IS 2 BP 130 EP 138 DI 10.5849/forsci.09-064 PG 9 WC Forestry SC Forestry GA 936WS UT WOS:000303621300004 ER PT J AU McIntosh, ACS Gray, AN Garman, SL AF McIntosh, Anne C. S. Gray, Andrew N. Garman, Steven L. TI Estimating Canopy Cover from Standard Forest Inventory Measurements in Western Oregon SO FOREST SCIENCE LA English DT Article DE crown closure; aerial photos; forest inventory and analysis; forest simulator; stocking equations ID PACIFIC-NORTHWEST; BASAL AREA; CASCADES; HABITAT; MODEL AB Reliable measures of canopy cover are important in the management of public and private forests. However, direct sampling of canopy cover is both labor- and time-intensive. More efficient methods for estimating percent canopy cover could be empirically derived relationships between more readily measured stand attributes and canopy cover or, alternatively, the use of aerial photos. In this study, we compared field-based measures of percent canopy cover with estimates from aerial photography, with equations of individual tree crown width and crown overlap used in the US Forest Service Forest Vegetation Simulator (FVS) equations and with models we developed from standard stand-level forest mensuration estimates. Standard inventory estimates of cover using 1:40,000 scale aerial photos were poorly correlated with field-measured cover, especially in wet hardwood (r = 0.60) and dry hardwood (r = 0.51) stands. FVS equations underestimated cover by 17% on average at high cover levels (>70%) in wet conifer and wet hardwood stands. We also developed predictive models of canopy cover for three forest groups sampled on 884 plots by the Forest Inventory and Analysis program in western Oregon: wet conifer, wet hardwood, and dry hardwood. Predictions by the models were within 15% of measured cover for >82% of the observations. Compared with previous studies modeling canopy cover, our best predictive models included species-specific stocking equations, whereas species-invariant basal area was not an important predictor for most forest types. Accuracies of these new predictive models may be adequate for some purposes, reducing the need for direct measures of canopy cover in the field. FOR. SCI. 58(2):154-167. C1 [McIntosh, Anne C. S.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2H1, Canada. [Gray, Andrew N.] US Forest Serv, Pacific NW Res Stn, Washington, DC USA. [Garman, Steven L.] Oregon State Univ, Dept Forest Sci, Corvallis, OR 97331 USA. RP McIntosh, ACS (reprint author), Univ Alberta, Dept Renewable Resources, 751 Gen Serv Bldg, Edmonton, AB T6G 2H1, Canada. EM amcintos@ualberta.ca; agray01@fs.fed.us; slgarman@usgs.gov FU US Forest Service Pacific Northwest Research Station; Oregon State University FX This project was funded through a joint venture agreement between the US Forest Service Pacific Northwest Research Station and Oregon State University. We thank Manuela Huso for statistical advice. We are also grateful to anonymous reviewers for their valuable comments on the manuscript. NR 47 TC 5 Z9 5 U1 3 U2 16 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD APR PY 2012 VL 58 IS 2 BP 154 EP 167 DI 10.5849/forsci.09-127 PG 14 WC Forestry SC Forestry GA 936WS UT WOS:000303621300006 ER PT J AU Hoffman, C Morgan, P Mell, W Parsons, R Strand, EK Cook, S AF Hoffman, Chad Morgan, Penelope Mell, William Parsons, Russell Strand, Eva K. Cook, Stephen TI Numerical Simulation of Crown Fire Hazard Immediately after Bark Beetle-Caused Mortality in Lodgepole Pine Forests SO FOREST SCIENCE LA English DT Article DE crown fire behavior; fire modeling; mountain pine beetle; computational fluid dynamics; heterogeneous fuels ID MOUNTAIN PINE; SPREAD; OUTBREAK; WILDFIRE; VEGETATION; PATTERNS; MOISTURE; FUELS AB Quantifying the effects of mountain pine beetle (MPB)-caused tree mortality on potential crown fire hazard has been challenging partly because of limitations in current operational fire behavior models. Such models are not capable of accounting for fuel heterogeneity resulting from an outbreak. Further, the coupled interactions between fuel, fire, and atmosphere are not modeled. To overcome these limitations, we used the Wild land-Urban Interface Fire Dynamics Simulator (WFDS) to investigate the influences of tree spatial arrangement and magnitude of MPB-caused tree mortality on simulated fire hazard. Field-collected, tree-level data from 1 1 sites were used to populate WFDS simulation domains representing a range of lodgepole pine forest structures for the postoutbreak period of time when dead needles are still present in the tree crowns. We found increases in the amount of crown fuel consumption and the intensity of crown fires as the percentage of MPB-caused tree mortality increased. In addition, we found complex interactions between the level of mortality, stand structure, and spatial arrangement of trees. These results suggest that preoutbreak forest structure and percent tree mortality influence crown fire behavior while dead needles are in the crown, and that the effect varies with spatial heterogeneity among trees. FOR. SCI. 58(2):178-188. C1 [Hoffman, Chad] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. [Morgan, Penelope; Strand, Eva K.] Univ Idaho, Coll Nat Resources, Wildland Fire Program, Moscow, ID 83843 USA. [Mell, William] US Forest Serv, Pacific Wildland Fire Sci Lab, Seattle, WA USA. [Parsons, Russell] US Forest Serv, Fire Sci Lab, Missoula, MT USA. [Cook, Stephen] Univ Idaho, Coll Agr & Life Sci, Dept Plant Soils & Entomol Sci, Moscow, ID 83843 USA. RP Hoffman, C (reprint author), Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. EM c.hoffman@colostate.edu; pmorgan@uidaho.edu; wemell@fs.fed.us; rparsons@fs.fed.us; evas@uidaho.edu; stephenc@uidaho.edu OI Hoffman, Chad/0000-0001-8715-937X FU Forest Health Protection Special Technology Development; US Forest Service; US Department of Agriculture [STDP R6-2008-04, 08-JV011060123-103] FX This research was supported in part by funds provided by Forest Health Protection Special Technology Development, the US Forest Service, and US Department of Agriculture STDP R6-2008-04 under Research Joint Venture Agreement 08-JV011060123-103. We appreciate the assistance of Erin Berryman and Harold Osborne in collecting all field data. We thank Helen Maffei, Eric Pfeiffer, and the rest of the staff on the Deschutes and Salmon-Challis National Forests for help in identifying field locations. NR 52 TC 23 Z9 23 U1 2 U2 19 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD APR PY 2012 VL 58 IS 2 BP 178 EP 188 DI 10.5849/forsci.10-137 PG 11 WC Forestry SC Forestry GA 936WS UT WOS:000303621300008 ER PT J AU Probst, C Cotty, PJ AF Probst, Claudia Cotty, Peter J. TI Relationships between in vivo and in vitro aflatoxin production: reliable prediction of fungal ability to contaminate maize with aflatoxins SO FUNGAL BIOLOGY LA English DT Article DE Aflatoxins; Aspergillus flavus; Maize; Synthetic media; Aetiology ID ASPERGILLUS-SECTION FLAVI; VEGETATIVE COMPATIBILITY; AGROECOLOGICAL-ZONES; SUBMERGED CULTURE; GENETIC DIVERSITY; UNITED-STATES; COTTONSEED; STRAINS; FIELD; PARASITICUS AB Aflatoxins are highly carcinogenic mycotoxins frequently produced by Aspergillus flavus. Contamination of maize with aflatoxins imposes both economic and health burdens in many regions. Identification of the most important etiologic agents of contamination is complicated by mixed infections and varying aflatoxin-producing potential of fungal species and individuals. In order to know the potential importance of an isolate to cause a contamination event, the ability of the isolate to produce aflatoxins on the living host must be determined. Aflatoxin production in vitro (synthetic and natural media) was contrasted with in vivo (viable maize kernels) in order to determine ability of in vitro techniques to predict the relative importance of causal agents to maize contamination events. Several media types and fermentation techniques (aerated, non-aerated, fermentation volume) were compared. There was no correlation between aflatoxin production in viable maize and production in any of the tested liquid fermentation media using any of the fermentation techniques. Isolates that produced aflatoxins on viable maize frequently failed to produce detectable (limit of detection = 1 ppb) aflatoxin concentrations in synthetic media. Aflatoxin production on autoclaved maize kernels was highly correlated with production on viable maize kernels. The results have important implications for researchers seeking to either identify causal agents of contamination events or characterize atoxigenic isolates for biological control. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Cotty, Peter J.] Univ Arizona, USDA ARS, Sch Plant Sci, Div Plant Pathol & Microbiol, Tucson, AZ 85721 USA. RP Cotty, PJ (reprint author), Univ Arizona, USDA ARS, Sch Plant Sci, Div Plant Pathol & Microbiol, POB 210036, Tucson, AZ 85721 USA. EM pjcotty@email.arizona.edu NR 48 TC 10 Z9 10 U1 1 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 J9 FUNGAL BIOL-UK JI Fungal Biol. PD APR PY 2012 VL 116 IS 4 BP 503 EP 510 DI 10.1016/j.funbio.2012.02.001 PG 8 WC Mycology SC Mycology GA 934ZQ UT WOS:000303486900004 PM 22483048 ER PT J AU Bolton, MD Secor, GA Rivera, V Weiland, JJ Rudolph, K Birla, K Rengifo, J Campbell, LG AF Bolton, Melvin D. Secor, Gary A. Rivera, Viviano Weiland, John J. Rudolph, Kurt Birla, Keshav Rengifo, Judith Campbell, Larry G. TI Evaluation of the potential for sexual reproduction in field populations of Cercospora beticola from USA SO FUNGAL BIOLOGY LA English DT Article DE Beta vulgaris; Cercospora beticola; Cercospora leaf spot; Mating type distribution; Mating type genes ID MATING-TYPE GENES; PATHOGEN SEPTORIA-PASSERINII; SUGAR-BEET; RHYNCHOSPORIUM-SECALIS; BETA-VULGARIS; PLANT-DISEASE; LEAF-SPOT; GENETICS; FUNGI; RECOMBINATION AB Cercospora leaf spot, caused by the hemibiotrophic fungal pathogen Cercospora beticola, is the most economically damaging foliar disease of sugarbeet worldwide. Although most C. beticola populations display characteristics reminiscent of sexual recombination, no teleomorph has been described. To assess whether populations in northern United States have characteristics consistent with sexual reproduction, 1024 isolates collected over a 3-y period were analyzed for frequency and distribution of mating type genes. After clone correction, an approximately equal distribution of mating types was found for each sampling year. Mating type frequency was also assessed in individual lesions. Lesions always consisted of isolates with a single mating type and microsatellite haplotype, but both mating types and up to five microsatellite haplotypes could be found on an individual leaf. The MAT1-1-1 and MAT1-2-1 genes were sequenced from 28 MAT1-1 and 28 MAT1-2 isolates, respectively. Three MAT1-1-1 nucleotide haplotypes were identified that encoded a single amino acid sequence. For MAT1-2-1, five nucleotide haplotypes were identified that encoded four protein variants. MAT1-1-1 and MAT1-2-1 gene expression analyses were conducted on plants inoculated with either or both mating types. MAT1-1-1 expression remained low, but MAT1-2-1 spiked during late stages of colonization. A segment of the MAT1-2-1 coding sequence was also found in MAT1-1 isolates. Taken together, these results suggest that C. beticola has the potential for sexual reproduction. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Bolton, Melvin D.; Weiland, John J.; Campbell, Larry G.] USDA ARS, No Crops Sci Lab, Fargo, ND 58102 USA. [Secor, Gary A.; Rivera, Viviano; Rudolph, Kurt; Birla, Keshav; Rengifo, Judith] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. RP Bolton, MD (reprint author), USDA ARS, No Crops Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM Melvin.Bolton@ars.usda.gov FU USDA-ARS CRIS [5442-22000-042-00D] FX This work was supported by USDA-ARS CRIS project 5442-22000-042-00D. We thank Xiaoyun Wang for excellent technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 62 TC 14 Z9 14 U1 0 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 J9 FUNGAL BIOL-UK JI Fungal Biol. PD APR PY 2012 VL 116 IS 4 BP 511 EP 521 DI 10.1016/j.funbio.2012.01.011 PG 11 WC Mycology SC Mycology GA 934ZQ UT WOS:000303486900005 PM 22483049 ER PT J AU Shappell, NW Mostrom, MS Lenneman, EM AF Shappell, N. W. Mostrom, M. S. Lenneman, E. M. TI E-Screen evaluation of sugar beet feedstuffs in a case of reduced embryo transfer efficiencies in cattle: the role of phytoestrogens and zearalenone SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Article DE MCF-7; Estrogenicity; Subfertility; Zearalenone; Sugar beets ID BREAST-CANCER CELLS; ESTROGENIC ACTIVITY; IN-VITRO; BETA-SITOSTEROL; DAIRY-COWS; GROWTH; EXTRACTION; ZERANOL; ALPHA; PULP AB The E-Screen assay was used to evaluate the estrogenicity of sugar beet by-products obtained from a dairy farm experiencing low success rates of embryo transfer. The beet tailings had similar to 3-fold the estradiol equivalents of the pelleted beet pulp (3.9 and 1.2 mu g estradiol equivalents or E(2)Eq/kg dry matter, respectively). Whole sugar beets, sugar beet pellets, and shreds from several Midwest US locations were also evaluated by E-Screen. All pellets examined were found to have some estrogenic activity (range similar to 0.1-2.0 mu g E(2)Eq/kg DM) with a mean of 0.46 mu g/kg dry matter and median of 0.28 mu g/kg dry matter. Relative E(2)Eq ranked as follows: pellets > shreds > most unprocessed roots. Using recommended feeding levels and conservative absorption estimates (10%), the estrogenic activity in the original samples could result in blood estradiol equivalents a parts per thousand yenaEuro parts per thousand those found at estrus (10 pg/mL, cows). Chemical analyses revealed no known phytoestrogens, but the estrogenic mycotoxin, zearalenone, was found in 15 of 21 samples. Of significance to those using the E-Screen are our findings that contradict previous reports: -sitosterol has no proliferative effect and genistein's glucuronidated form-genistin-is equal to genistein in proliferative effect. The latter is the result of deconjugation of genistin to genistein in the presence of fetal bovine serum (determined by LC MSMS). These data show the usefulness and caveats of the E-Screen in evaluation of feedstuffs, and indicate a potential for sugar beet by-products to contain zearalenone at concentrations that may impact reproduction. C1 [Shappell, N. W.; Lenneman, E. M.] ARS, USDA, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND 58105 USA. [Mostrom, M. S.] N Dakota State Univ, Vet Diagnost Serv Dept, Fargo, ND 58105 USA. RP Shappell, NW (reprint author), ARS, USDA, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58105 USA. EM nancy.shappell@ars.usda.gov NR 57 TC 4 Z9 4 U1 0 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD APR PY 2012 VL 48 IS 4 BP 216 EP 228 DI 10.1007/s11626-012-9489-9 PG 13 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 934XD UT WOS:000303479200003 ER PT J AU Patel, J Macarisin, D Sanglay, G Murphy, C AF Patel, Jitendra Macarisin, Dumitru Sanglay, Gabriel Murphy, Charles TI Inactivation and injury of pathogens on intact beef treated with hydrodynamic pressure SO INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES LA English DT Article DE Listeria monocytogenes; Escherichia coli 0157:H7; Salmonella Typhimurium; Hydrodynamic pressure; Beef; Inactivation; Injury ID HIGH HYDROSTATIC-PRESSURE; ESCHERICHIA-COLI O157-H7; LISTERIA-MONOCYTOGENES; MEMBRANE DAMAGE; MICROORGANISMS; SALMONELLA; ATTACHMENT; ADHERENCE; SURFACES; BACTERIA AB The effect of hydrodynamic pressure (HDP) treatment on the inactivation of Escherichia cob 0157:H7, Salmonella Typhimurium, and Listeria monocytogenes attached to beef surface was evaluated. Irradiated beef cubes were inoculated with these individual pathogens (ca. 4 log CFU.g(-1)) and treated with HOP. Surviving populations of E. coli 0157:H7, S. Typhimurium, and L monocytogenes were determined by spiral-plating on Sorbitol MacConkey agar (SMAC), XLT4 agar, and modified Oxford (MOX) agar, respectively. The HOP treatment significantly reduced E. coli 0157:H7 (0.75 log CFU.g(-1)), S. Typhimurium (1.09 log CFU.g(-1)), and L monocytogenes (0.55 log CFU.g(-1)) from beef surfaces. HOP-induced injury as determined by plating on non-selective media revealed that ca. 0.5 log CFU.g(-1) of E. coli 0157:H7 and S. Typhimurium cells were injured. Most cell injury occurred at the cell membrane level as observed by transmission electron microscopy (TEM). Industrial relevance: Results obtained in the present study show that HDP treatment, primarily evaluated for meat tenderness improvement, also reduces the number of viable pathogens attached to beef surface. Additional hurdle treatment will be helpful to further reduce pathogens on beef surface. Published by Elsevier Ltd. C1 [Patel, Jitendra; Macarisin, Dumitru] ARS, USDA, Environm & Microbial Food Safety Lab, Beltsville, MD 20705 USA. [Sanglay, Gabriel] Ohio State Univ, Dept Food Sci & Technol, Columbus, OH 43210 USA. [Murphy, Charles] ARS, Elect Microscope Unit, USDA, Beltsville, MD 20705 USA. RP Patel, J (reprint author), ARS, USDA, Environm & Microbial Food Safety Lab, Beltsville, MD 20705 USA. EM jitu.patel@ars.usda.gov; dumitru.macarisin@ars.usda.gov; sanglay.1@buckeyemail.osu.edu; charles.murphy@ars.usda.gov NR 32 TC 3 Z9 4 U1 2 U2 8 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1466-8564 EI 1878-5522 J9 INNOV FOOD SCI EMERG JI Innov. Food Sci. Emerg. Technol. PD APR PY 2012 VL 14 BP 38 EP 45 DI 10.1016/j.ifset.2011.08.002 PG 8 WC Food Science & Technology SC Food Science & Technology GA 937AO UT WOS:000303631600006 ER PT J AU Davis, RE Zhao, Y Dally, EL Jomantiene, R Lee, IM Wei, W Kitajima, EW AF Davis, Robert E. Zhao, Yan Dally, Ellen L. Jomantiene, Rasa Lee, Ing-Ming Wei, Wei Kitajima, Elliot W. TI 'Candidatus Phytoplasma sudamericanum', a novel taxon, and strain PassWB-Br4, a new subgroup 16SrIII-V phytoplasma, from diseased passion fruit (Passiflora edulis f. flavicarpa Deg.) SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID WITCHES-BROOM; CLASSIFICATION; GENES AB Symptoms of abnormal proliferation of shoots resulting in formation of witches'-broom growths were observed on diseased plants of passion fruit (Passiflora edulis f. flavicarpa Deg.) in Brazil. RFLP analysis of 16S rRNA gene sequences amplified in PCRs containing template DNAs extracted from diseased plants collected in Bonito (Pernambuco) and Vicosa (Minas Gerais) Brazil, indicated that such symptoms were associated with infections by two mutually distinct phytoplasmas. One phytoplasma, PassWB-Br4 from Bonito, represents a new subgroup, 16SrIII-V, in the X-disease phytoplasma group ('Candidatus Phytoplasma pruni'-related strains). The second phytoplasma, PassWB-Br3 from Vicosa, represents a previously undescribed subgroup in group 16SrVI. Phylogenetic analyses of 16S rRNA gene sequences were consistent with the hypothesis that strain PassWB-Br3 is distinct from previously described 'Ca. Phytoplasma' species. Nucleotide sequence alignments revealed that strain PassWB-Br3 shared less than 97.5% 16S rRNA gene sequence similarity with previously described 'Ca. Phytoplasma' species. The unique properties of its DNA, in addition to natural host and geographical occurrence, support the recognition of strain PassWB-Br3 as a representative of a novel taxon, 'Candidatus Phytoplasma sudamericanum'. C1 [Davis, Robert E.; Zhao, Yan; Dally, Ellen L.; Jomantiene, Rasa; Lee, Ing-Ming; Wei, Wei] USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Jomantiene, Rasa] Nat Res Ctr, Phytovirus Lab, Vilnius, Lithuania. [Wei, Wei] Univ Maryland, Inst Biotechnol, Ctr Biosyst Res, Rockville, MD 20850 USA. [Kitajima, Elliot W.] Univ Sao Paulo, Dept Fitopatol & Nematol, Escola Super Agr Luiz de Queiroz, BR-13418900 Sao Paulo, Brazil. RP Davis, RE (reprint author), USDA ARS, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. EM robert.davis@ars.usda.gov NR 29 TC 15 Z9 15 U1 0 U2 4 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD APR PY 2012 VL 62 BP 984 EP 989 DI 10.1099/ijs.0.033423-0 PN 4 PG 6 WC Microbiology SC Microbiology GA 934ZR UT WOS:000303487100041 PM 21669919 ER PT J AU Zhang, SY Duan, JG Strelkoff, TS Bautista, E AF Zhang, Shiyan Duan, Jennifer G. Strelkoff, Theodor S. Bautista, Eduardo TI Simulation of Unsteady Flow and Soil Erosion in Irrigation Furrows SO JOURNAL OF IRRIGATION AND DRAINAGE ENGINEERING-ASCE LA English DT Article DE Irrigation furrows; Unsteady flow; Infiltration; Sediment transport ID ZERO-INERTIA; SEDIMENT TRANSPORT; SURFACE IRRIGATION; MODEL; ADVANCE; DEPTH; BED AB This study developed a one-dimensional numerical model for the simulation of unsteady flow and the resultant soil erosion in irrigation furrows. The model solves a modified version of the Saint-Venant equations that consider the loss of mass and momentum attributable to infiltration and sediment transport. The transport rate of fine sediment was predicted with a modified Laursen formula that treats the tractive shear stress as a function of both Reynolds number and the particle size. The modified Laursen formula was verified by using the erosion data measured in the field and in a laboratory flume. The model accurately predicted flow advance times and outflow hydrographs in comparison with data measured in irrigation furrows at Kimberly, Idaho. Sediment discharge predictions were less accurate. DOI: 10.1061/(ASCE)IR.1943-4774.0000396. (C) 2012 American Society of Civil Engineers. C1 [Zhang, Shiyan; Duan, Jennifer G.] Univ Arizona, Dept Civil Engn & Engn Mech, Tucson, AZ 85721 USA. [Zhang, Shiyan] Tsinghua Univ, Grad Sch Shenzhen, Shenzhen 518055, Peoples R China. [Strelkoff, Theodor S.; Bautista, Eduardo] ARS, Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85238 USA. RP Duan, JG (reprint author), Univ Arizona, Dept Civil Engn & Engn Mech, Tucson, AZ 85721 USA. EM gduan@email.arizona.edu FU U.S. Department of Agriculture, Agricultural Research Service, Arid Land Agricultural Research Center [58-5347-8-413] FX This work is a result of collaborative research sponsored by the U.S. Department of Agriculture, Agricultural Research Service, Arid Land Agricultural Research Center, under Specific Agreement Number 58-5347-8-413. Thanks to Ms. Bai Yang and Ms. Anu Acharya who assisted with the experiments. NR 26 TC 2 Z9 2 U1 1 U2 9 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9437 J9 J IRRIG DRAIN E-ASCE JI J. Irrig. Drainage Eng-ASCE PD APR PY 2012 VL 138 IS 4 BP 294 EP 303 DI 10.1061/(ASCE)IR.1943-4774.0000396 PG 10 WC Agricultural Engineering; Engineering, Civil; Water Resources SC Agriculture; Engineering; Water Resources GA 933FB UT WOS:000303344100002 ER PT J AU Kumcu, A AF Kumcu, Aylin TI No longer tax exempt: income tax calculation in the Consumer Expenditure Survey SO MONTHLY LABOR REVIEW LA English DT Article AB BLS has developed an experimental federal income tax calculator that estimates taxes for Consumer Expenditure Survey participants to help address high nonresponse rates, unreliable reporting, and time-frame ambiguity; this calculator could improve the quality of the aftertax income data in the survey C1 [Kumcu, Aylin] US Bur Labor Stat, Washington, DC 20212 USA. RP Kumcu, A (reprint author), Econ Res Serv, USDA, Washington, DC USA. EM akumcu@ers.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU LEGAL BOOKS DEPOT PI LOS ANGELES PA PO BOX 27789, LOS ANGELES, CA 90027 USA SN 0098-1818 J9 MON LABOR REV JI Mon. Labor Rev. PD APR PY 2012 VL 135 IS 4 BP 44 EP 57 PG 14 WC Industrial Relations & Labor SC Business & Economics GA 937HE UT WOS:000303648800004 ER PT J AU Arakane, Y Lomakin, J Gehrke, SH Hiromasa, Y Tomich, JM Muthukrishnan, S Beeman, RW Kramer, KJ Kanost, MR AF Arakane, Yasuyuki Lomakin, Joseph Gehrke, Stevin H. Hiromasa, Yasuaki Tomich, John M. Muthukrishnan, Subbaratnam Beeman, Richard W. Kramer, Karl J. Kanost, Michael R. TI Formation of Rigid, Non-Flight Forewings (Elytra) of a Beetle Requires Two Major Cuticular Proteins SO PLOS GENETICS LA English DT Article ID TRIBOLIUM-CASTANEUM; ANOPHELES-GAMBIAE; COMPOSITE STRUCTURES; BOMBYX-MORI; R CONSENSUS; CUTICLE; GENE; ANNOTATION; SILKWORM; FAMILIES AB Insect cuticle is composed primarily of chitin and structural proteins. To study the function of structural cuticular proteins, we focused on the proteins present in elytra (modified forewings that become highly sclerotized and pigmented covers for the hindwings) of the red flour beetle, Tribolium castaneum. We identified two highly abundant proteins, TcCPR27 (10 kDa) and TcCPR18 (20 kDa), which are also present in pronotum and ventral abdominal cuticles. Both are members of the Rebers and Riddiford family of cuticular proteins and contain RR2 motifs. Transcripts for both genes dramatically increase in abundance at the pharate adult stage and then decline quickly thereafter. Injection of specific double-stranded RNAs for elytra of the resulting adults, however, were shorter, wrinkled, warped, fenestrated, and less rigid than those from control insects. TcCPR27-deficient insects could not fold their hindwings properly and died prematurely approximately one week after eclosion, probably because of dehydration. TcCPR18-deficient insects exhibited a similar but less dramatic phenotype. Immunolocalization studies confirmed the presence of TcCPR27 in the elytral cuticle. These results demonstrate that TcCPR27 and TcCPR18 are major structural proteins in the rigid elytral, dorsal thoracic, and ventral abdominal cuticles of the red flour beetle, and that both proteins are required for morphogenesis of the beetle's elytra. C1 [Arakane, Yasuyuki; Hiromasa, Yasuaki; Tomich, John M.; Muthukrishnan, Subbaratnam; Kramer, Karl J.; Kanost, Michael R.] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. [Arakane, Yasuyuki] Chonnam Natl Univ, Div Plant Biotechnol, Coll Agr & Life Sci, Kwangju, South Korea. [Lomakin, Joseph; Gehrke, Stevin H.] Univ Kansas, Dept Chem & Petr Engn, Lawrence, KS 66045 USA. [Beeman, Richard W.; Kramer, Karl J.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. RP Arakane, Y (reprint author), Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. EM arakane@chonnam.ac.kr FU National Science Foundation [IOS0726425, MRI0521587] FX This work was supported by National Science Foundation grants IOS0726425 and MRI0521587 (http://www.nsf.gov/index.jsp). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 41 TC 17 Z9 18 U1 1 U2 18 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD APR PY 2012 VL 8 IS 4 BP 612 EP 622 AR e1002682 DI 10.1371/journal.pgen.1002682 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA 934KA UT WOS:000303441800046 PM 22570623 ER PT J AU Webster, TM Nichols, RL AF Webster, Theodore M. Nichols, Robert L. TI Changes in the Prevalence of Weed Species in the Major Agronomic Crops of the Southern United States: 1994/1995 to 2008/2009 SO WEED SCIENCE LA English DT Review DE Economically important weeds; troublesome weeds; survey; weed distributions; weed population shifts ID GLYPHOSATE-RESISTANT CROPS; AMARANTH AMARANTHUS-PALMERI; COTTON GOSSYPIUM-HIRSUTUM; COMMELINA-BENGHALENSIS CONTROL; HERBICIDE USE PATTERNS; LONG-TERM TILLAGE; CONSERVATION TILLAGE; GROWER SURVEY; COVER CROPS; GEORGIA AB Changes in the weed flora of cropping systems reflect the impacts of factors that create safe sites for weed establishment and facilitate the influx and losses to and from the soil seedbank. This analysis of the annual surveys of the Southern Weed Science Society documents changes in the weed flora of the 14 contiguous southern states since the advent of transgenic, herbicide-resistant crops. In 1994 and 2009, the top five weeds in corn were morningglories, Texas millet, broadleaf signalgrass, johnsongrass, and sicklepod; in this same period Palmer amaranth, smartweeds, and goosegrass had the greatest increases in importance in corn. In cotton, morningglories and nutsedges were among the top five most troublesome weeds in 1995 and 2009. Palmer amaranth, pigweeds, and Florida pusley were also among the five most troublesome species in 2009; the weeds with the largest increases in importance in cotton were common ragweed and two species with tolerance to glyphosate, Benghal dayflower and Florida pusley. In soybean, morningglories, nutsedges, and sicklepod were among the top five weed species in 1995 and 2009. Two species with glyphosate resistance, Palmer amaranth and horseweed, were the second and fourth most troublesome weeds of soybean in 2009. In wheat, the top four weeds in 2008 were the same as those in 1994 and included Italian ryegrass, wild garlic, wild radish, and henbit. Crop production in the southern region is a mosaic of various crop rotations, soil types, and types of tillage. During the interval between the surveys, the predominant change in weed management practices in the region and the nation was the onset and rapid dominance of the use of glyphosate in herbicide-resistant cultivars of corn, cotton, and soybean. Because of the correspondence between the effects of glyphosate on the respective weed species and the observed changes in the weed flora of the crops, it is likely the very broad use of glyphosate was a key component shaping the changes in weed flora. Only eight of the top 15 most troublesome weeds of cotton and soybean, the crops with the greatest use of glyphosate, were the same in 1995 and 2009. In contrast, in corn and wheat where adoption of glyphosate-resistant cultivars lags or is absent, 12 of the 15 most troublesome weeds were the same in 1994 and 2008. These findings show on a regional scale that weeds adapt to recurrent selection from herbicides, currently the predominant weed management tool. Future research should seek methods to hinder the rapid spread of herbicide-tolerant and evolution of herbicide-resistant weed species. As new tools are developed, research should focus on ways to preserve the efficacy of those tools through improved stewardship. Nomenclature: annual bluegrass, Poa annua L. POAAN; Benghal dayflower, Commelina benghalensis L. COMBE; broadleaf signalgrass, Urochloa platyphylla (Nash) R.D. Webster BRAPP; common ragweed, Ambrosia artemisiifolia L. AMBEL; Florida pusley Richardia scabra L. RCHSC; goosegrass Eleusine indica (L.) Gaertn. ELEIN; groundcherries, Physalis spp.; henbit, Lamium amplexicaule L. LAMAM; horseweed, Conyza canadensis (L.) Cronq. ERICA; Italian ryegrass, Lolium perenne L. ssp. multiflorum (Lam.) Husnot LOLMU; johnsongrass, Sorghum halepense (L.) Pers. SORHA; morningglories, Ipomoea spp.; nutsedges, Cyperus spp.; Palmer amaranth, Amaranthus palmeri S. Wats. AMAPA; pigweed, Amaranthus spp.; sicklepod, Senna obtusifolia (L.) H.S. Irwin & Barneby CASOB; smartweeds, Polygonum spp.; Texas millet, Urochloa texana (Buckl.) R. Webster PANTE; wild garlic, Allium vineale L. ALLVI; wild radish, Raphanus raphanistrum L. RAPRA; corn, Zea mays L.; cotton, Gossypium hirsutum L.; soybean Glycine max. (L.) Merr.; wheat, Triticum aestivum L. C1 [Webster, Theodore M.] ARS, Crop Protect & Management Res Unit, USDA, Tifton, GA 31794 USA. [Nichols, Robert L.] Cotton Inc, Cary, NC 27513 USA. RP Webster, TM (reprint author), ARS, Crop Protect & Management Res Unit, USDA, Tifton, GA 31794 USA. EM ted.webster@ars.usda.gov RI Webster, Theodore/A-4468-2009 OI Webster, Theodore/0000-0002-8259-2059 NR 69 TC 31 Z9 32 U1 6 U2 70 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2012 VL 60 IS 2 BP 145 EP 157 DI 10.1614/WS-D-11-00092.1 PG 13 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 934EJ UT WOS:000303425900002 ER PT J AU Moriles, J Hansen, S Horvath, DP Reicks, G Clay, DE Clay, SA AF Moriles, Janet Hansen, Stephanie Horvath, David P. Reicks, Graig Clay, David E. Clay, Sharon A. TI Microarray and Growth Analyses Identify Differences and Similarities of Early Corn Response to Weeds, Shade, and Nitrogen Stress SO WEED SCIENCE LA English DT Article DE Weed competition; genomics; transcriptome analysis; critical weed free period ID CRITICAL PERIOD; LIGHT QUALITY; COMPETITION; PLANT; INTERFERENCE; MAIZE; SET; BIOSYNTHESIS; KNOWLEDGE; FOXTAIL AB Weed interference with crop growth is often attributed to water, nutrient, or light competition; however, specific physiological responses to these stresses are not well described. This study's objective was to compare growth, yield, and gene expression responses of corn to nitrogen (N), low light (40% shade), and weed stresses. Corn vegetative parameters from V2 to V12 stages, yield parameters, and gene expression using transcriptome (2008) and quantitative polymerase chain reaction (qPCR) (2008/09) analyses at V8 were compared among the stresses and with nonstressed corn. N stress did not affect vegetative parameters, although grain yield was reduced by 40% compared with nonstressed plants. Shade, present until V2, reduced biomass and leaf area > 50% at V2, and recovering plants remained smaller than nonstressed plants at V12. However, grain yields of shade-stressed and nonstressed plants were similar, unless shade remained until V8. Weed stress reduced corn growth and yield in 2008 when weeds remained until V6. In 2009, weed stress until V2 reduced corn vegetative growth, but yield reductions occurred only if weed stress remained until V6 or later. Principle component analysis of differentially expressed genes indicated that shade and weed stress had more similar gene expression patterns to each other than they did to nonstressed or N-stressed tissues. However, corn grown in N-stressed conditions shared 252 differentially expressed genes with weed-stressed plants. Ontologies associated with light/photosynthesis, energy conversion, and signaling were down-regulated in response to all three stresses. Shade and weed stress clustered most tightly together, based on gene expression, but shared only three ontologies, O-METHYLTRANSFERASE activity (lignification processes), POLY(U)-BINDING activity (posttranscriptional gene regulation), and stomatal movement. Based on morphologic and genomic observations, weed stress to corn was not explained by individual effects of N or light stress. Therefore, we hypothesize that these stresses share limited signaling mechanisms. Nomenclature: Corn, Zea mays L. C1 [Moriles, Janet; Hansen, Stephanie; Reicks, Graig; Clay, David E.; Clay, Sharon A.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. ARS, USDA, Fargo, ND 58102 USA. RP Clay, SA (reprint author), S Dakota State Univ, Dept Plant Sci, 1110 Rotunda Lane, Brookings, SD 57007 USA. EM sharon.clay@sdstate.edu OI Horvath, David/0000-0002-8458-7691 FU South Dakota Agricultural Experiment Station; South Dakota Corn Utilization Council; South Dakota Soybean Research and Promotion Council; USDA-CSREES [2008-34556-19350]; South Dakota 2010 initiative; USDA-CSREES-NRI [2009-35320-05040] FX Support for this project was provided by the South Dakota Agricultural Experiment Station, South Dakota Corn Utilization Council, South Dakota Soybean Research and Promotion Council, a USDA-CSREES-seed technology grant (2008-34556-19350), the South Dakota 2010 initiative, and a USDA-CSREES-NRI grant (2009-35320-05040). NR 42 TC 8 Z9 9 U1 2 U2 28 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2012 VL 60 IS 2 BP 158 EP 166 DI 10.1614/WS-D-11-00090.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 934EJ UT WOS:000303425900003 ER PT J AU Nandula, VK Reddy, KN Koger, CH Poston, DH Rimando, AM Duke, SO Bond, JA Ribeiro, DN AF Nandula, Vijay K. Reddy, Krishna N. Koger, Clifford H. Poston, Daniel H. Rimando, Agnes M. Duke, Stephen O. Bond, Jason A. Ribeiro, Daniela N. TI Multiple Resistance to Glyphosate and Pyrithiobac in Palmer Amaranth (Amaranthus palmeri) from Mississippi and Response to Flumiclorac SO WEED SCIENCE LA English DT Article DE Absorption; AMPA; antagonism; shikimate; translocation ID TRANSLOCATION; ACCUMULATION; MECHANISM; ARKANSAS; GEORGIA; MSMA AB Greenhouse and laboratory studies were conducted to confirm and quantify glyphosate resistance, quantify pyrithiobac resistance, and investigate interaction between flumiclorac and glyphosate mixtures on control of Palmer amaranth from Mississippi. The GR(50) (herbicide dose required to cause a 50% reduction in plant growth) values for two glyphosate-resistant biotypes, C1B1 and T4B1, and a glyphosate-susceptible (GS) biotype were 1.52, 1.3, and 0.09 kg ae ha(-1) glyphosate, respectively. This indicated that the C1B1 and T4B1 biotypes were 17- and 14-fold resistant to glyphosate, respectively, compared with the GS biotype. The C1B1 and T4B1 biotypes were also resistant to pyrithiobac, an acetolactate synthase (ALS) inhibitor, with GR(50) values of 0.06 and 0.07 kg ai ha(-1), respectively. This indicated that the C1B1 and T4B1 biotypes were 7- and 8-fold, respectively, more resistant to pyrithiobac compared with the GS biotype, which had a GR(50) value of 0.009 kg ha(-1). Flumiclorac was antagonistic to glyphosate by reducing glyphosate translocation. The C1B1 and T4B1 absorbed less glyphosate 48 h after treatment (HAT) compared with the GS biotype. The majority of the translocated glyphosate accumulated in the shoot above the treated leaf (that contains the apical meristem) in the GS biotype and in the shoot below the treated leaf in the resistant biotypes, C1B1 and T4B1, by 48 HAT. The C1B1 biotype accumulated negligible shikimate levels, whereas the T4B1 and GS biotypes recorded elevated levels of shikimate. Metabolism of glyphosate to aminomethylphosphonic acid was not detected in either of the resistant biotypes or the susceptible GS biotype. The above results confirm multiple resistance to glyphosate and pyrithiobac in Palmer amaranth biotypes from Mississippi and indicate that resistance to glyphosate is partly due to reduced absorption and translocation of glyphosate. Nomenclature: Flumiclorac; glyphosate; pyrithiobac; Palmer amaranth, Amaranthus palmeri S. Wats. AMAPA. C1 [Nandula, Vijay K.; Reddy, Krishna N.] ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. [Koger, Clifford H.] Syngenta Crop Protect Inc, Indianola, MS 38751 USA. [Poston, Daniel H.] Pioneer Hi Bred Int So Business Unit, Huntsville, AL 35802 USA. [Rimando, Agnes M.; Duke, Stephen O.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Bond, Jason A.] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA. [Ribeiro, Daniela N.] Mississippi State Univ, Dept Plant & Soil Sci, Mississippi State, MS 39762 USA. RP Nandula, VK (reprint author), ARS, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. EM vijay.nandula@ars.usda.gov NR 23 TC 31 Z9 31 U1 1 U2 14 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2012 VL 60 IS 2 BP 179 EP 188 DI 10.1614/WS-D-11-00157.1 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 934EJ UT WOS:000303425900006 ER PT J AU Cerdeira, AL Cantrell, CL Dayan, FE Byrd, JD Duke, SO AF Cerdeira, Antonio L. Cantrell, Charles L. Dayan, Franck E. Byrd, John D. Duke, Stephen O. TI Tabanone, a New Phytotoxic Constituent of Cogongrass (Imperata cylindrica) SO WEED SCIENCE LA English DT Article DE Allelochemical; allelopathy; phytotoxin; essential oil ID INHIBITORY ACTIVITY; GROWTH; POPULATIONS; HERBICIDE; GRASS AB Cogongrass is a troublesome, invasive weedy species with reported allelopathic properties. The phytotoxicity of different constituents isolated from roots and aerial parts of this species was evaluated on garden lettuce and creeping bentgrass. No significant phytotoxic activity was detected in the methylene chloride, methanol, or water extracts when tested at 1.0 mg ml(-1). However, the total essential oil extract of cogongrass aerial parts was active. Bioactivity-guided fractionation of this extract using silica gel column chromatography led to the identification of megastigmatrienone, 4-(2-butenylidene)-3,5,5-trimethyl-2-cyclohexen-1-one (also called tabanone), as a mixture of four stereoisomers responsible for most of the activity. Tabanone inhibited growth of frond area of lesser duckweed, root growth of garden onion, and fresh weight gain of garden lettuce with 50% inhibition values of 0.094, 3.6, and 6.5 mM, respectively. The target site of tabanone is not known, but its mode of action results in rapid loss of membrane integrity and subsequent reduction in the rate of photosynthetic electron flow. Nomenclature: Tabanone, 4-(2-butenylidene)-3,5,5-trimethyl-2-cyclohexen-1-one; cogongrass, Imperata cylindrica (L.) Beauv. IMCY; creeping bentgrass, Agrostis stolonifera L.; lesser duckweed, Lemna aequinoctialis Welw.; garden lettuce, Lactuca sativa L.; garden onion, Allium cepa L. C1 [Cantrell, Charles L.; Dayan, Franck E.; Duke, Stephen O.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Cerdeira, Antonio L.] EMBRAPA Environm, Brazilian Dept Agr, Agr Res Serv, BR-13820000 Sao Paulo, Brazil. [Byrd, John D.] Mississippi State Univ, Mississippi State, MS 39762 USA. RP Duke, SO (reprint author), ARS, USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. EM Stephen.Duke@ars.usda.gov RI Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 NR 29 TC 7 Z9 8 U1 1 U2 28 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2012 VL 60 IS 2 BP 212 EP 218 DI 10.1614/WS-D-11-00160.1 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 934EJ UT WOS:000303425900011 ER PT J AU Bicksler, AJ Masiunas, JB Davis, A AF Bicksler, Abram J. Masiunas, John B. Davis, Adam TI Canada Thistle (Cirsium arvense) Suppression by Sudangrass Interference and Defoliation SO WEED SCIENCE LA English DT Article DE Competition; cover crop; mowing; mulch; perennial weed ID WEED MANAGEMENT; COVER CROPS; SORGHUM; ALLELOPATHY; COMPETITION; SORGOLEONE; SYSTEMS; GROWTH; STRATEGIES; IMPACT AB Canada thistle is difficult to manage in organic farming systems and others with reduced reliance on herbicides. Previous field studies found that defoliation or sudangrass interference suppressed Canada thistle. Our objective was to understand the factors causing suppression of Canada thistle observed in the field. Three greenhouse studies were conducted utilizing frequency of defoliation, sudangrass interference and defoliation, and interspecific phytotoxicity to discern mechanisms of Canada thistle suppression. Increased defoliation frequency (up to four defoliations) decreased Canada thistle shoot height, shoot and root mass, and root-to-shoot ratio. Plants with larger root mass had greater shoot mass and number (r = 0.87 and 0.73, respectively), indicating a probable interdependence of root size (carbohydrate reserves), bud density, and subsequent shoot growth. In the sudangrass interference and defoliation study, Canada thistle shoot dry mass was 38.7, 2.76, and 0.39 g pot(-1) in the defoliation only, sudangrass interference only, and defoliation + interference + surface mulch treatments, respectively. Sudangrass interference by itself was effective in suppressing thistle growth; combining interference with defoliation did not further reduce growth (2.76 and 2.83 g pot(-1), respectively). In the experiment minimizing interspecific competition, we found no evidence of sudangrass having a phytotoxic effect on Canada thistle. Overall results indicate that sudangrass competition or frequent shoot removal suppresses growth of Canada thistle. Nomenclature: Canada thistle, Cirsium arvense (L.) Scop.; sudangrass, Sorghum bicolor (L.) Moench spp. drummondii (Nees ex Steud.) de Wet and Harlan. C1 [Bicksler, Abram J.; Masiunas, John B.; Davis, Adam] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Davis, Adam] ARS, USDA, Global Change & Photosynthesis Res Unit, Urbana, IL 61801 USA. RP Masiunas, JB (reprint author), Univ Illinois, Dept Crop Sci, 1201 W Gregory Dr, Urbana, IL 61801 USA. EM masiunas@illinois.edu FU Hatch project [60011-802050-802336]; North Central Sustainable Agriculture Research and Education (SARE) [490215-802050-19110] FX We thank Craig Anderson, Steve Bossu, Lauren Flowers, Matt Luedtke, Chris Gittings and Kyle Rak for their invaluable assistance. The research was partially funded through Hatch project number 60011-802050-802336 and North Central Sustainable Agriculture Research and Education (SARE) project number 490215-802050-19110. NR 37 TC 1 Z9 1 U1 4 U2 20 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2012 VL 60 IS 2 BP 260 EP 266 DI 10.1614/WS-D-11-00145.1 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 934EJ UT WOS:000303425900017 ER PT J AU Tasker, AV Westwood, JH AF Tasker, Alan V. Westwood, James H. TI The U.S. Witchweed Eradication Effort Turns 50: A Retrospective and Look-Ahead on Parasitic Weed Management SO WEED SCIENCE LA English DT Editorial Material C1 [Westwood, James H.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Tasker, Alan V.] US Anim & Plant Hlth Inspect Serv, USDA, Silver Spring, MD 20905 USA. RP Westwood, JH (reprint author), Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, 401 Latham Hall, Blacksburg, VA 24061 USA. EM westwood@vt.edu NR 1 TC 2 Z9 2 U1 2 U2 21 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2012 VL 60 IS 2 BP 267 EP 268 DI 10.1614/WS-D-12-00003.1 PG 2 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 934EJ UT WOS:000303425900018 ER PT J AU Strunk, JL Reutebuch, SE Andersen, HE Gould, PJ McGaughey, RJ AF Strunk, Jacob L. Reutebuch, Stephen E. Andersen, Hans-Erik Gould, Peter J. McGaughey, Robert J. TI Model-Assisted Forest Yield Estimation with Light Detection and Ranging SO WESTERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE forest inventory; design-based; LiDAR; model-assisted; regression estimation ID LASER SCANNER DATA; BIOPHYSICAL PROPERTIES; STAND CHARACTERISTICS; ABOVEGROUND BIOMASS; LIDAR; INVENTORY; DENSITY; OREGON; VOLUME; LEVEL AB Previous studies have demonstrated that light detection and ranging (LiDAR)-derived variables can be used to model forest yield variables, such as biomass, volume, and number of stems. However, the next step is underrepresented in the literature: estimation of forest yield with appropriate confidence intervals. It is of great importance that the procedures required for conducting forest inventory with LiDAR and the estimation precision of such procedures are sufficiently documented to enable their evaluation and implementation by land managers. In this study, we demonstrated the regression estimator, a model-assisted estimator (approximately design-unbiased), using LiDAR-derived variables for estimation of total forest yield. The LiDAR-derived variables are statistics associated with vegetation height and cover. The estimation procedure requires complete coverage of the forest with LiDAR and a random sample of precisely georeferenced field measurement plots. Regression estimation relies on sample-based ordinary least squares (OLS) regression models relating forest yield and LiDAR-derived variables. Estimation was performed using the OLS models and LiDAR-derived variables for the entire population. Regression estimates of basal area, volume, stand density, and biomass were much more precise than simple random sampling estimates (design effects were 0.25, 0.24, 0.44, and 0.27, respectively). C1 [Strunk, Jacob L.] Oregon State Univ, Coll Forestry, Corvallis, OR 97330 USA. [Reutebuch, Stephen E.; Andersen, Hans-Erik; McGaughey, Robert J.] US Forest Serv, Pacific NW Res Stn, Seattle, WA 98195 USA. [Gould, Peter J.] US Forest Serv, Pacific NW Res Stn, Olympia, WA 98512 USA. RP Strunk, JL (reprint author), Oregon State Univ, Coll Forestry, 204 Peavy Hall, Corvallis, OR 97330 USA. EM jacob.strunk@oregonstate.edu RI Duello, Theresa/P-5752-2015 NR 39 TC 10 Z9 12 U1 3 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0885-6095 J9 WEST J APPL FOR JI West. J. Appl. For. PD APR PY 2012 VL 27 IS 2 BP 53 EP 59 DI 10.5849/wjaf.10-043 PG 7 WC Forestry SC Forestry GA 933ZN UT WOS:000303407200001 ER PT J AU Lowell, EC Dykstra, DP Monserud, RA AF Lowell, Eini C. Dykstra, Dennis P. Monserud, Robert A. TI Evaluating Effects of Thinning on Wood Quality in Southeast Alaska SO WESTERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE precommercial thinning; thinning; wood quality; Sitka spruce; hemlock; second-growth timber ID SITKA SPRUCE; WESTERN HEMLOCK; BLACK SPRUCE; GROWTH; MANAGEMENT; STANDS; VALUES; LUMBER; FIR AB We examined the effect of thinning on wood quality of western hemlock (Tsuga heterophylla) and Sitka spruce (Picea sitchensis) located on Prince of Wales and Mitkof Islands in southeast Alaska. Sample trees came from paired plots (thinned versus unthinned) in eight naturally regenerated, mixed stands of young-growth western hemlock and Sitka spruce in an effort to examine a range of thinning densities, from 10 x 10 ft to 20 x 20 ft spacing. The stands, which had been thinned in the late 1970s and early 1980s, ranged in age from 36 to 73 years at the time they were selected for this study in 2003. The main focus of the stand selection in this retrospective study was to find individual stands that included a thinned plot and an adjacent unthinned plot from the same original stand. A random sample of trees from both thinned and unthinned plots was selected, stratified by tree dbh. About 12 trees per species per stand (461 trees in total) were selected for the study. Selected trees were harvested, and a lumber recovery study was conducted, enabling us to relate log volume to recovered lumber volume by product grade. Surfaced dry dimension lumber was produced, graded, and nondestructively tested using the transverse E-vibration standard test for stiffness. We did not evaluate the resource for appearance-grade products. For each species, the overall treatment effect (thinned versus unthinned) on lumber grade recovery and transverse E-vibration modulus of elasticity by vertical-log position (butt log, middle log, or top log) were analyzed using a mixed-effects procedure. Results suggest that there were no significant differences in product recovery or value between the thinned plots and the untreated control plots in the manufacture of structural lumber products. C1 [Lowell, Eini C.] US Forest Serv, Pacific NW Res Stn, Portland Forestry Sci Lab, Portland, OR 97205 USA. RP Lowell, EC (reprint author), US Forest Serv, Pacific NW Res Stn, Portland Forestry Sci Lab, 620 SW Main St,Suite 400, Portland, OR 97205 USA. EM elowell@fs.fed.us FU US Forest Service Region 10 FX Eini C. Lowell (elowell@fs.fed.us), US Forest Service, Pacific Northwest Research Station, Portland Forestry Sciences Laboratory, 620 SW Main Street, Suite 400, Portland, OR 97205. Dennis P. Dykstra (retired) and Robert A. Monserud (retired), US Forest Service, Pacific Northwest Research Station, Portland, OR 97205. This study was funded by US Forest Service Region 10. We thank Jim Russell (retired) and Gary Lawton, Forest Service Alaska Region, for their generous contributions of time and personnel. Also, we appreciate the stand data collection work coordinated by Mike McClellan and performed by personnel from the Pacific Northwest Research Station's Juneau Forestry Sciences Laboratory and the work of Kevin Curtis and his colleagues at the Ketchikan Wood Technology Center in log processing and lumber testing. Special thanks go to Pat Cunningham, Pacific Northwest Statistics Unit, for his help in analyzing the data. NR 47 TC 0 Z9 0 U1 2 U2 13 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0885-6095 J9 WEST J APPL FOR JI West. J. Appl. For. PD APR PY 2012 VL 27 IS 2 BP 72 EP 83 DI 10.5849/wjaf.11-019 PG 12 WC Forestry SC Forestry GA 933ZN UT WOS:000303407200003 ER PT J AU Driscoll, CT Lambert, KF Chapin, FS Nowak, DJ Spies, TA Swanson, FJ Kittredge, DB Hart, CM AF Driscoll, Charles T. Lambert, Kathleen F. Chapin, F. Stuart, III Nowak, David J. Spies, Thomas A. Swanson, Frederick J. Kittredge, David B. Hart, Clarisse M. TI Science and Society: The Role of Long-Term Studies in Environmental Stewardship SO BIOSCIENCE LA English DT Article DE boundary spanning; environmental policy and management; Long Term Ecological Research Network; science communication ID CHANGING CLIMATE; LAND-USE; FOREST; MASSACHUSETTS; ECOSYSTEM; DISTURBANCE; STRATEGIES; POLICY; USA; CONSERVATION AB Long-term research should play a crucial role in addressing grand challenges in environmental stewardship. We examine the efforts of five Long Term Ecological Research Network sites to enhance policy, management, and conservation decisions for forest ecosystems. In these case studies, we explore the approaches used to inform policy on atmospheric deposition, public land management, land conservation, and urban forestry, including decision maker engagement and integration of local knowledge, application of models to analyze the potential consequences of policy and management decisions, and adaptive management to generate new knowledge and incorporate it into decisionmaking. Efforts to enhance the role of long-term research in informing major environmental challenges would benefit from the development of metrics to evaluate impact; stronger partnerships among research sites, professional societies, decisionmakers, and journalists; and greater investment in efforts to develop, test, and expand practice-based experiments at the interface of science and society. C1 [Driscoll, Charles T.] Syracuse Univ, Dept Civil & Environm Engn, New York, NY USA. [Lambert, Kathleen F.; Hart, Clarisse M.] Harvard Univ Harvard Forest, Petersham, MA USA. [Chapin, F. Stuart, III] Univ Alaska, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Nowak, David J.] US Forest Serv, USDA, No Res Stn, Syracuse, NY USA. [Spies, Thomas A.; Swanson, Frederick J.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Kittredge, David B.] Univ Massachusetts Amherst, Dept Environm Conservat, Amherst, MA USA. RP Driscoll, CT (reprint author), Syracuse Univ, Dept Civil & Environm Engn, New York, NY USA. EM ctdrisco@syr.edu RI Driscoll, Charles/F-9832-2014; OI Driscoll, Charles/0000-0003-2692-2890 FU Harvard University; Highstead; US National Science Foundation; US Forest Service FX The effort and contributions of KFL were generously supported by the Bullard Fellowship program of Harvard University's Harvard Forest and by a grant from Highstead. This is a contribution of the Long Term Ecological Research Network, which is supported by the US National Science Foundation and by the US Forest Service. We appreciate the efforts of David R. Foster in shepherding this series of articles. NR 61 TC 19 Z9 19 U1 1 U2 45 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD APR PY 2012 VL 62 IS 4 BP 354 EP 366 DI 10.1525/bio.2012.62.4.7 PG 13 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 927RK UT WOS:000302927600007 ER PT J AU Thompson, JR Wiek, A Swanson, FJ Carpenter, SR Fresco, N Hollingsworth, T Spies, TA Foster, DR AF Thompson, Jonathan R. Wiek, Arnim Swanson, Frederick J. Carpenter, Stephen R. Fresco, Nancy Hollingsworth, Teresa Spies, Thomas A. Foster, David R. TI Scenario Studies as a Synthetic and Integrative Research Activity for Long-Term Ecological Research SO BIOSCIENCE LA English DT Article DE socioecological systems; science synthesis; participatory engagement; futures ID LAND-USE; SCIENCE; OREGON AB Scenario studies have emerged as a powerful approach for synthesizing diverse forms of research and for articulating and evaluating alternative socioecological futures. Unlike predictive modeling, scenarios do not attempt to forecast the precise or probable state of any variable at a given point in the future. Instead, comparisons among a set of contrasting scenarios are used to understand the systemic relationships and dynamics of complex socioecological systems and to define a range of possibilities and uncertainties in quantitative and qualitative terms. We describe five examples of scenario studies affiliated with the US Long Term Ecological Research (LTER) Network and evaluate them in terms of their ability to advance the LTER Network's capacity for conducting science, promoting social and ecological science synthesis, and increasing the saliency of research through sustained outreach activities. We conclude with an argument that scenario studies should be advanced programmatically within large socioecological research programs to encourage prescient thinking in an era of unprecedented global change. C1 [Thompson, Jonathan R.] Smithsonian Inst, Conservat Biol Inst, Front Royal, VA USA. [Wiek, Arnim] Arizona State Univ, Sch Sustainabil, Tempe, AZ USA. [Swanson, Frederick J.; Spies, Thomas A.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Carpenter, Stephen R.] Univ Wisconsin, Ctr Limnol, Madison, WI 53706 USA. [Fresco, Nancy] Univ Alaska, Sch Nat Resources & Agr Sci, Fairbanks, AK 99701 USA. [Hollingsworth, Teresa] Univ Alaska, Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99701 USA. [Hollingsworth, Teresa] US Forest Serv, Pacific NW Res Stn, Fairbanks, AK USA. [Foster, David R.] Harvard Univ Harvard Forest, Petersham, MA USA. RP Thompson, JR (reprint author), Smithsonian Inst, Conservat Biol Inst, Front Royal, VA USA. EM thompsonjr@si.edu FU US National Science Foundation Long Term Ecological Research Network; US Forest Service FX Support for this work was provided by US National Science Foundation Long Term Ecological Research Network awards to Harvard University, Arizona State University, Oregon State University, the University of Alaska, and the University of Wisconsin and by the US Forest Service. NR 46 TC 26 Z9 26 U1 4 U2 46 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD APR PY 2012 VL 62 IS 4 BP 367 EP 376 DI 10.1525/bio.2012.62.4.8 PG 10 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 927RK UT WOS:000302927600008 ER PT J AU Jones, JA Creed, IF Hatcher, KL Warren, RJ Adams, MB Benson, MH Boose, E Brown, WA Campbell, JL Covich, A Clow, DW Dahm, CN Elder, K Ford, CR Grimm, NB Henshaw, DL Larson, KL Miles, ES Miles, KM Sebestyen, SD Spargo, AT Stone, AB Vose, JM Williams, MW AF Jones, Julia A. Creed, Irena F. Hatcher, Kendra L. Warren, Robert J. Adams, Mary Beth Benson, Melinda H. Boose, Emery Brown, Warren A. Campbell, John L. Covich, Alan Clow, David W. Dahm, Clifford N. Elder, Kelly Ford, Chelcy R. Grimm, Nancy B. Henshaw, Donald L. Larson, Kell L. Miles, Evan S. Miles, Kathleen M. Sebestyen, Stephen D. Spargo, Adam T. Stone, Asa B. Vose, James M. Williams, Mark W. TI Ecosystem Processes and Human Influences Regulate Streamflow Response to Climate Change at Long-Term Ecological Research Sites SO BIOSCIENCE LA English DT Article DE precipitation/runoff ratio; trend; succession; socioecological systems; Budyko curve ID WESTERN UNITED-STATES; EXPERIMENTAL FOREST; RIVER FLOWS; OSCILLATION; DYNAMICS; TRENDS; PRECIPITATION; VARIABILITY; CATCHMENT; EXTREMES AB Analyses of long-term records at 35 headwater basins in the United States and Canada indicate that climate change effects on streamflow are not as clear as might be expected, perhaps because of ecosystem processes and human influences. Evapotranspiration was higher than was predicted by temperature in water-surplus ecosystems and lower than was predicted in water-deficit ecosystems. Streamflow was correlated with climate variability indices (e.g., the El Nino Southern Oscillation, the Pacific Decadal Oscillation, the North Atlantic Oscillation), especially in seasons when vegetation influences are limited. Air temperature increased significantly at 17 of the 19 sites with 20- to 60-year records, but streamflow trends were directly related to climate trends (through changes in ice and snow) at only 7 sites. Past and present human and natural disturbance, vegetation succession, and human water use can mimic, exacerbate, counteract, or mask the effects of climate change on streamflow, even in reference basins. Long-term ecological research sites are ideal places to disentangle these processes. C1 [Jones, Julia A.; Miles, Evan S.] Oregon State Univ, Corvallis, OR 97331 USA. [Creed, Irena F.] Univ Western Ontario, London, ON, Canada. [Warren, Robert J.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. [Adams, Mary Beth] US Forest Serv, No Res Stn, Parsons, WV USA. [Benson, Melinda H.; Dahm, Clifford N.] Univ New Mexico, Albuquerque, NM 87131 USA. [Boose, Emery] Harvard Univ Harvard Forest, Petersham, MA USA. [Boose, Emery] Harvard Forest LTER Site, Boston, MA USA. [Brown, Warren A.] Cornell Univ, Cornell Inst Social & Econ Res, Ithaca, NY USA. [Campbell, John L.] USFS, NE Res Stn, Durham, NH USA. [Covich, Alan] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Clow, David W.] US Geol Survey, Colorado Water Sci Ctr, Lakewood, CO 80225 USA. [Dahm, Clifford N.] CALFED Bay Delta Program, Cleveland, OH USA. [Elder, Kelly] USFS, Rocky Mt Res Stn, Ft Collins, CO USA. [Ford, Chelcy R.; Vose, James M.] USFS, So Res Stn, Coweeta Hydrol Lab, Otto, NC USA. [Grimm, Nancy B.] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. [Henshaw, Donald L.] USFS, Pacific NW Res Stn, Corvallis, OR USA. [Larson, Kell L.] Arizona State Univ, Sch Geog Sci & Urban Planning, Tempe, AZ USA. [Sebestyen, Stephen D.] USFS, No Res Stn, Grand Rapids, MI USA. [Spargo, Adam T.] Univ Western Ontario, Dept Biol, London, ON, Canada. [Stone, Asa B.] Cent New Mexico Community Coll, Albuquerque, NM USA. [Williams, Mark W.] Univ Colorado, Denver, CO 80202 USA. RP Jones, JA (reprint author), Oregon State Univ, Corvallis, OR 97331 USA. EM jonesj@geo.oregonstate.edu RI Grimm, Nancy/D-2840-2009; Creed, Irena/L-8810-2013; Sebestyen, Stephen/D-1238-2013; OI Miles, Evan/0000-0001-5446-8571; Grimm, Nancy/0000-0001-9374-660X; Creed, Irena/0000-0001-8199-1472; Sebestyen, Stephen/0000-0002-6315-0108; Campbell, John/0000-0003-4956-1696; Clow, David/0000-0001-6183-4824 FU US Long Term Ecological Research (LTER) Network; National Science Foundation; Natural Sciences and Engineering Research Council of Canada; Networks of Centres of Excellence-Sustainable Forest Management Network FX Funding for this work was provided by the US Long Term Ecological Research (LTER) Network and National Science Foundation grants to participating sites and by a Natural Sciences and Engineering Research Council of Canada Discovery Grant to IFC. We thank LTER Network information managers for the creation and maintenance of ClimDB/HydroDB; the US Forest Service for the initial establishment and continued support of climate and basin measurements at many of the study sites; the US Geological Survey (USGS) Water, Energy, and Biogeochemical Budgets program, the USGS Hydrologic Benchmark Network, and the USGS National Water Information Service for provision of data; and the Networks of Centres of Excellence-Sustainable Forest Management Network-funded project on HydroEcological Landscapes and Processes (HELP) and the participating Canadian experimental basins from which data were contributed to the HELP project (Peter Tschaplinski for CAR, Tom Clair for KEJ, Fred Beall for TLW and MRM, Ray Hesslein for ELA, Peter Dillon for DOR, and Rita Winkler for UPC). We thank Merryl Albers, David R. Foster, Eveleyn Gaiser, Ann Giblin, Stephen P. Loheide II, Randy K. Kolka, Richard V. Pouyat, Sylvia Schaefer, Emily H. Stanley, Frederick J. Swanson, Will Wollheim, and three anonymous reviewers for comments on the manuscript. NR 55 TC 49 Z9 50 U1 7 U2 82 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD APR PY 2012 VL 62 IS 4 BP 390 EP 404 DI 10.1525/bio.2012.62.4.10 PG 15 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 927RK UT WOS:000302927600010 ER PT J AU Fountain, AG Campbell, JL Schuur, EAG Stammerjohn, SE Williams, MW Ducklow, HW AF Fountain, Andrew G. Campbell, John L. Schuur, Edward A. G. Stammerjohn, Sharon E. Williams, Mark W. Ducklow, Hugh W. TI The Disappearing Cryosphere: Impacts and Ecosystem Responses to Rapid Cryosphere Loss SO BIOSCIENCE LA English DT Article DE cryosphere; ecosystem response; environmental observatories ID CLIMATE-CHANGE; ANTARCTIC PENINSULA; LONG-TERM; MIXEDGRASS PRAIRIE; PERMAFROST THAW; SOUTHERN-OCEAN; SNOW; ICE; TRENDS; SOIL AB The cryosphere-the portion of the Earth's surface where water is in solid form for at least one month of the year-has been shrinking in response to climate warming. The extents of sea ice, snow, and glaciers, for example, have been decreasing. In response, the ecosystems within the cryosphere and those that depend on the cryosphere have been changing. We identify two principal aspects of ecosystem-level responses to cryosphere loss: (1) trophodynamic alterations resulting from the loss of habitat and species loss or replacement and (2) changes in the rates and mechanisms of biogeochemical storage and cycling of carbon and nutrients, caused by changes in physical forcings or ecological community functioning. These changes affect biota in positive or negative ways, depending on how they interact with the cryosphere. The important outcome, however, is the change and the response the human social system (infrastructure, food, water, recreation) will have to that change. C1 [Fountain, Andrew G.] Portland State Univ, Dept Geol, Portland, OR 97207 USA. [Campbell, John L.] US Forest Serv, USDA, Durham, NH USA. [Schuur, Edward A. G.] Univ Florida, Dept Biol, Gainesville, FL USA. [Stammerjohn, Sharon E.] Univ Calif Santa Cruz, Dept Ocean Sci, Santa Cruz, CA 95064 USA. [Williams, Mark W.] Univ Colorado, Dept Geog, Boulder, CO 80309 USA. [Ducklow, Hugh W.] Marine Biol Lab, Ctr Ecosyst, Woods Hole, MA 02543 USA. RP Fountain, AG (reprint author), Portland State Univ, Dept Geol, Portland, OR 97207 USA. EM andrew@pdx.edu OI Campbell, John/0000-0003-4956-1696 FU National Science Foundation's (NSF) Long Term Ecological Research (LTER) Network [OPP 0823101, OPP 1115245, DEB 1114804, DEB-1026415, DEB-0620579, DEB-1027341] FX The authors wish to thank the funding provided by the National Science Foundation's (NSF) Long Term Ecological Research (LTER) Network for supporting our long-term studies, in which we track the ecosystem response to the disappearing cryosphere. The LTER Network office supported the workshop from which this article is derived. Table 2 and figure 3 were kindly contributed by Julia A. Jones, Kendra L. Hatcher, and Evan S. Miles. Valuable contributions were made by Mike Antolin, Anne Giblin, John Hobble, John Magnusson, Anne Nolin, Bruce Peterson, Bill Sobczak, and Will Wolheim. We greatly appreciate all who participated. Fred Swanson offered many helpful suggestions, and David Foster carefully edited the manuscript (and it is much improved). NSF LTER Site Grants OPP 0823101, OPP 1115245, DEB 1114804, DEB-1026415, DEB-0620579, and DEB-1027341 supported the authors during the preparation of this article. NR 61 TC 23 Z9 24 U1 6 U2 61 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD APR PY 2012 VL 62 IS 4 BP 405 EP 415 DI 10.1525/j.bio.2012.62.4.11 PG 11 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 927RK UT WOS:000302927600011 ER PT J AU Richardson, BA Meyer, SE AF Richardson, Bryce A. Meyer, Susan E. TI Paleoclimate effects and geographic barriers shape regional population genetic structure of blackbrush (Coleogyne ramosissima: Rosaceae) SO BOTANY-BOTANIQUE LA English DT Article DE amplified fragment length polymorphisms; biogeography; Colorado Plateau; Mojave; postglacial colonization ID MULTILOCUS GENOTYPE DATA; MOJAVE-DESERT; POSTGLACIAL COLONIZATION; SEED-GERMINATION; COLORADO PLATEAU; VEGETATION; DNA; MARKERS; INDIVIDUALS; QUATERNARY AB Coleogyne ramosissima Torr. (blackbrush) is a dominant xerophytic shrub species in the ecotone between the warm and cold deserts of interior western North America. Amplified fragment length polymorphisms (AFLPs) were used to survey genetic diversity and population genetic structure at 14 collection sites across the species range. Analysis revealed significant population differentiation (F-ST = 0.103, p < 0.0001) and reasonably high levels of genetic diversity (expected heterozygosity; H-E = 0.26), a surprising result for a putative paleoendemic species. Model-based Bayesian clustering, principal coordinates analysis, and neighbor-joining analysis all produced support for the existence of two metapopulations, the first centered on the Mojave Desert and the second on the Colorado Plateau. These genetic data, coupled with information from Late Pleistocene and Holocene packrat (genus Neotoma Say and Ord, 1825) middens, illustrate a demographic history in which eastern and western distributions were disjunct during the Last Glacial Maximum and remained so through the Holocene, forming the present-day metapopulations in the Mojave Desert and Colorado Plateau. This strong regional genetic differentiation has implications for population persistence and migration in response to future climate change, as well as for shrubland restoration following anthropogenic disturbances such as annual grass invasion and wildfire. C1 [Richardson, Bryce A.; Meyer, Susan E.] US Forest Serv, USDA, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. RP Richardson, BA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, Shrub Sci Lab, 735 North,500 East, Provo, UT 84606 USA. EM brichardson02@fs.fed.us FU USDA Forest Service, Rocky Mountain Research Station, USDA Forest Service National Fire Plan [2012-NFP-GSD-1] FX We thank Gerald Rehfeldt, Stephanie Carlson, Julia Schumann, and Erin Heckaman for technical assistance, Stephanie Carlson, Fred Edwards, Todd Esque, Josh Hoines, Ed Kleiner, Burton Pendleton, Jane Rogers, and Bettina Schultz for providing help with seed collection, and Stewart Sanderson for review of the manuscript. Funding for this project was provided by USDA Forest Service, Rocky Mountain Research Station, USDA Forest Service National Fire Plan (2012-NFP-GSD-1). NR 35 TC 4 Z9 4 U1 0 U2 17 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1916-2790 J9 BOTANY JI Botany PD APR PY 2012 VL 90 IS 4 BP 293 EP 299 DI 10.1139/B2012-002 PG 7 WC Plant Sciences SC Plant Sciences GA 932TA UT WOS:000303312200005 ER PT J AU Brunet, J Larson-Rabin, Z AF Brunet, Johanne Larson-Rabin, Zachary TI The response of flowering time to global warming in a high-altitude plant: the impact of genetics and the environment SO BOTANY-BOTANIQUE LA English DT Article DE global warming; flowering time; phenotypic plasticity; genetic differentiation; Aquilegia coerulea; high-altitude habitats ID PHENOTYPIC PLASTICITY; CLIMATE-CHANGE; POLLINATOR INTERACTIONS; EVOLUTIONARY RESPONSES; IMPATIENS-CAPENSIS; AQUILEGIA-CAERULEA; LATITUDINAL CLINE; ARABIDOPSIS; PHENOLOGY; DIFFERENTIATION AB In high-altitude habitats, an increase in temperature and greater precipitation in the form of rain represent climate changes typically associated with global warming. We determined whether phenotypic plasticity and genetic changes in the mean phenotype could affect the adaptation of flowering time to changes in the environment resulting from global warming in a montane plant species, Aquilegia coerulea James. We collected seeds from 17 plants from each of three natural populations. For each of these 51 families, we assigned 3-4 individuals to each of four water and temperature treatments. We observed phenotypic plasticity in flowering time in response to both temperature and water availability but no genetic variance or genetic differentiation in phenotypic plasticity. These results indicate that phenotypic plasticity could provide a quick response to environmental changes but provides little evolutionary potential. In contrast to phenotypic plasticity in flowering time, the mean flowering time did vary among families and among populations, suggesting a genetic basis to flowering time and adaptation in the different populations. The most likely scenario for the adaptation of this plant species to climate change is a rapid response via phenotypic plasticity followed by selection and micro-evolutionary changes in the mean phenotype. C1 [Brunet, Johanne] Univ Wisconsin, USDA ARS, VCRU, Dept Entomol, Madison, WI 53706 USA. RP Brunet, J (reprint author), Univ Wisconsin, USDA ARS, VCRU, Dept Entomol, 1630 Linden Dr, Madison, WI 53706 USA. EM Johanne.Brunet@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX Christy Stewart, Kirstin Martin, Jesse Koyen, Evan Eifler, Alex Rhamlow, and John Rehbeck helped with data collection. Comments from David Inouye and anonymous reviewers improved the manuscript. Funding for this project was provided by the United States Department of Agriculture, Agricultural Research Service to J.B. NR 42 TC 2 Z9 2 U1 5 U2 54 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 1916-2790 J9 BOTANY JI Botany PD APR PY 2012 VL 90 IS 4 BP 319 EP 326 DI 10.1139/B2012-001 PG 8 WC Plant Sciences SC Plant Sciences GA 932TA UT WOS:000303312200008 ER PT J AU Lee, JE Wei, EK Fuchs, CS Hunter, DJ Lee, IM Selhub, J Stampfer, MJ Willett, WC Ma, J Giovannucci, E AF Lee, Jung Eun Wei, Esther K. Fuchs, Charles S. Hunter, David J. Lee, I-Min Selhub, Jacob Stampfer, Meir J. Willett, Walter C. Ma, Jing Giovannucci, Edward TI Plasma folate, methylenetetrahydrofolate reductase (MTHFR), and colorectal cancer risk in three large nested case-control studies SO CANCER CAUSES & CONTROL LA English DT Article DE Folate; Methylenetetrahydrofolate reductase (MTHFR); Colorectal cancer ID UNMETABOLIZED FOLIC-ACID; COMMON MUTATION; DIETARY-FOLATE; PUBLIC-HEALTH; COLON-CANCER; FORTIFICATION; METAANALYSIS; ADENOMA; GENE; HOMOCYSTEINE AB Few prospective studies have examined the associations between blood levels of folate, in conjunction with methylenetetrahydrofolate reductase (MTHFR) polymorphisms, and colorectal cancer. We evaluated the associations between plasma folate, MTHFR C677T, and A1298C, and colorectal cancer in three large prospective studies: the Nurses' Health Study, the Health Professionals Follow-up Study, and the Physicians' Health Study. A total of 602 incident cases were identified and individually matched to controls who provided blood specimens. We used conditional logistic regression to calculate the relative risk (RR) and 95% confidence interval (95% CI) and then pooled the estimates using a random effects model. We found a lower risk of colorectal cancer among participants with low plasma folate levels: compared with the lowest quartile, RRs (95% CIs) for each successively higher quartile of plasma folate levels were 1.55 (1.14-2.11), 1.37 (1.00-1.88), and 1.47 (1.07-2.01; P for trend = 0.10). For the MTHFR polymorphisms, RRs (95% CIs) were 0.62 (0.44-0.90) for 677TT versus CC/CT and 0.68 (0.31-1.51) for 1298CC versus AC/AA, and these lower-risk genotypes were associated with lower circulating plasma folate levels. When we partitioned the variation in plasma folate levels, variation due to folate intake was not positively associated with colorectal cancer risk. We found that low plasma folate levels were associated with lower risk of colorectal cancer. The reasons underlying a lower risk of colorectal cancer with low plasma folate levels require elucidation because plasma folate levels can reflect dietary intake, genetic influences, and other factors. C1 [Wei, Esther K.; Fuchs, Charles S.; Hunter, David J.; Stampfer, Meir J.; Willett, Walter C.; Ma, Jing; Giovannucci, Edward] Brigham & Womens Hosp, Channing Lab, Dept Med, Boston, MA 02115 USA. [Lee, Jung Eun] Sookmyung Womens Univ, Dept Food & Nutr, Seoul, South Korea. [Wei, Esther K.] Calif Pacific Med Ctr, Res Inst, San Francisco, CA USA. [Fuchs, Charles S.] Dana Farber Canc Inst, Dept Med Oncol, Boston, MA 02115 USA. [Hunter, David J.; Lee, I-Min; Stampfer, Meir J.; Willett, Walter C.; Giovannucci, Edward] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Lee, I-Min] Brigham & Womens Hosp, Dept Med, Div Prevent Med, Boston, MA 02115 USA. [Selhub, Jacob] Tufts Univ, Human Nutr Res Ctr Aging, Jean Mayer US Dept Agr, Boston, MA 02111 USA. RP Giovannucci, E (reprint author), Brigham & Womens Hosp, Channing Lab, Dept Med, 181 Longwood Ave, Boston, MA 02115 USA. EM edward.giovannucci@channing.harvard.edu OI Lee, Jung Eun/0000-0003-1141-878X FU National Cancer Institute [CA87969, CA55075, CA42182, CA34944, CA40360, CA97193]; National Heart, Lung, and Blood Institute [HL26490, HL34595]; Prevent Cancer Foundation FX Our study is supported by National Cancer Institute CA87969, CA55075, CA42182, CA34944, CA40360, and CA97193, National Heart, Lung, and Blood Institute HL26490 and HL34595, and Prevent Cancer Foundation. The sponsors of the study had no role in the study design, data collection, data analysis, data interpretation, or writing of the report. NR 34 TC 21 Z9 21 U1 0 U2 8 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0957-5243 J9 CANCER CAUSE CONTROL JI Cancer Causes Control PD APR PY 2012 VL 23 IS 4 BP 537 EP 545 DI 10.1007/s10552-012-9911-3 PG 9 WC Oncology; Public, Environmental & Occupational Health SC Oncology; Public, Environmental & Occupational Health GA 931QE UT WOS:000303233600002 PM 22367721 ER PT J AU Qi, ZM Bartling, PNS Ahuja, LR Derner, JD Dunn, GH Ma, LW AF Qi, Zhiming Bartling, Patricia N. S. Ahuja, Lajpat R. Derner, Justin D. Dunn, Gale H. Ma, Liwang TI Development and evaluation of the carbon-nitrogen cycle module for the GPFARM-Range model SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE GPFARM-Range; Carbon-nitrogen cycle; Modeling; Rangelands; NLEAP; Java ID NORTH-AMERICAN GRASSLANDS; FORAGE PRODUCTION; NLEAP MODEL; SIMULATION; SOIL; CLIMATE; SYSTEMS; PERFORMANCE; MANAGEMENT; RESPONSES AB Rangelands cover approximately 50% of the terrestrial surface of the earth. Consequently, the soil carbon and nitrogen storage and turnover in rangeland systems are becoming increasingly important for sustainable grazing management and climate change. In this study, a carbon-nitrogen (C-N) cycle module from the N Leaching and Environmental Analysis Package (NLEAP) was added into the GPFARM-Range model. This linkage was tested against a 14-year forage dataset (1993-2006) with measurements of C and N in 1993, 2003, and 2006 near Cheyenne, WY. The results showed that the peak standing crop (PSC), and changes in soil organic carbon (SOC) and total soil organic nitrogen (TON) in the rangeland were reasonably simulated by the new GPFARM-Range model. The indices of agreement (d) were >0.85 and the mean bias errors (MBE) were <130 kg ha(-1) for the PSC simulation. The SOC and TON in the soil profile were simulated with relative root mean squared error (RRMSE) <5% for the calibration treatment without grazing and <20% for the validation treatments with grazing, both showing no significant bias or error from the observed values. A sensitivity analysis showed that the model responded reasonably to changes in temperature and precipitation. A long-term dataset with more soil C and N measurement events on the rangeland is needed to further test the model, but it is not available at this time. Additional investigation is needed into the adequacy of root-to-shoot ratio approaches to simulate root growth for forage groups in these systems. Published by Elsevier B.V. C1 [Qi, Zhiming; Bartling, Patricia N. S.; Ahuja, Lajpat R.; Dunn, Gale H.; Ma, Liwang] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. [Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. RP Qi, ZM (reprint author), USDA ARS, Agr Syst Res Unit, 2150 Ctr Ave,Bldg D-200, Ft Collins, CO 80526 USA. EM Zhiming.Qi@ars.usda.gov NR 35 TC 6 Z9 7 U1 1 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD APR PY 2012 VL 83 BP 1 EP 10 DI 10.1016/j.compag.2012.01.007 PG 10 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 931LH UT WOS:000303220900001 ER PT J AU Li, XH Lee, WS Li, MZ Ehsani, R Mishra, AR Yang, CH Mangan, RL AF Li, Xiuhua Lee, Won Suk Li, Minzan Ehsani, Reza Mishra, Ashish Ratn Yang, Chenghai Mangan, Robert L. TI Spectral difference analysis and airborne imaging classification for citrus greening infected trees SO COMPUTERS AND ELECTRONICS IN AGRICULTURE LA English DT Article DE Airborne imaging; Citrus greening; Disease detection; Image classification; Spectral analysis ID RED EDGE POSITION; REFLECTANCE; MANAGEMENT; REMOVAL; DISEASE; IMAGERY; FLORIDA AB Citrus greening, also called Huanglongbing (HLB), became a devastating disease spread through citrus groves in Florida, since it was first found in 2005. Multispectral (MS) and hyperspectral (HS) airborne images of citrus groves in Florida were acquired to detect citrus greening infected trees in 2007 and 2010. Ground truthing including field and indoor spectral measurement, infection status along with GPS coordinates was conducted for both healthy and infected trees. Ground spectral measurements showed that healthy canopy had higher reflectance in the visible range, and lower reflectance in the near-infrared (NIR) range than HLB infected canopy. Red edge position (REP) also showed notable difference between healthy and HLB canopy. But the difference in the NIR range and REP were comparably more sensitive to the environment or the background noise. Accuracy for separating HLB and healthy samples reached more than 90% when a simple REP threshold method was implemented in the ground reflectance datasets, regardless of field or indoor measurement; but it did not work well with the HS images because of its low spatial resolution. Support vector machine (SVM) was able to provide a fast, easy and adoptable way to build a mask for tree canopy. High positioning error of the ground truth in the 2007 HS image led to validation accuracy of less than 50% for most of classification methods. In the 2010 image from Southern Gardens (SG) grove, with better ground truth records, higher classification accuracies (about 90% in training sets, more than 60% in validation sets for most of the methods) were achieved. Disease density maps were also generated from the classification results of each method; most of them were able to identify the severely infected areas. Simpler classification methods such as minimum distance (MinDist) and Mahalanobis distance (MahaDist) showed more stable and balanced detection accuracy between the training and validation sets in the 2010 images. Their similar infection trend with ground scouted maps showed a promising future to manage HLB disease with airborne spectral imaging. (c) 2012 Elsevier B.V. All rights reserved. C1 [Li, Xiuhua; Lee, Won Suk] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Li, Xiuhua; Li, Minzan] China Agr Univ, MOE, Key Lab Modern Precis Agr Integrat Res, Beijing 100083, Peoples R China. [Ehsani, Reza; Mishra, Ashish Ratn] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Yang, Chenghai; Mangan, Robert L.] USDA ARS, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Lee, WS (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM wslee@ufl.edu FU Citrus Research and Development Foundation, Inc. FX The authors thank Ms. Ce Yang, Mr. Asish Skaria, Mr. Ferhat Kurtulmus, Mr. Anurag Katti, and Mr. John Simmons in the Precision Agriculture Laboratory, Agricultural and Biological Engineering Department, University of Florida for their kind support and assistance on the ground truthing experiment, statistical analysis, MATLAB programming, etc. This project was funded by the Citrus Research and Development Foundation, Inc. NR 40 TC 28 Z9 36 U1 5 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0168-1699 J9 COMPUT ELECTRON AGR JI Comput. Electron. Agric. PD APR PY 2012 VL 83 BP 32 EP 46 DI 10.1016/j.compag.2012.01.010 PG 15 WC Agriculture, Multidisciplinary; Computer Science, Interdisciplinary Applications SC Agriculture; Computer Science GA 931LH UT WOS:000303220900004 ER PT J AU Gazzani, G Grusak, MA AF Gazzani, Gabriella Grusak, Michael A. TI Functional foods and their expanding applications in the improvement of human health SO CURRENT OPINION IN BIOTECHNOLOGY LA English DT Editorial Material C1 [Gazzani, Gabriella] Univ Pavia, Dept Drug Sci, I-27100 Pavia, Italy. [Grusak, Michael A.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. RP Gazzani, G (reprint author), Univ Pavia, Dept Drug Sci, Viale Taramelli 12, I-27100 Pavia, Italy. EM gabriella.gazzani@unipv.it NR 2 TC 1 Z9 1 U1 0 U2 9 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0958-1669 J9 CURR OPIN BIOTECH JI Curr. Opin. Biotechnol. PD APR PY 2012 VL 23 IS 2 BP 127 EP 128 DI 10.1016/j.copbio.2012.01.009 PG 2 WC Biochemical Research Methods; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA 931LF UT WOS:000303220700001 PM 22321939 ER PT J AU Spear, SF Crisafulli, CM Storfer, A AF Spear, Stephen F. Crisafulli, Charles M. Storfer, Andrew TI Genetic structure among coastal tailed frog populations at Mount St. Helens is moderated by post-disturbance management SO ECOLOGICAL APPLICATIONS LA English DT Article DE amphibians; Ascaphus truei; coastal tailed frog; disturbance; inbreeding; landscape genetics; Mount St. Helens, Washington, USA; natural regeneration vs. management; recolonization; salvage logging ID COARSE WOODY DEBRIS; DOUGLAS-FIR FORESTS; LANDSCAPE GENETICS; ASCAPHUS-TRUEI; INFREQUENT DISTURBANCES; DIFFERENTIATION MEASURE; AMPHIBIAN DECLINES; DIVERSITY; CONSERVATION; CONSEQUENCES AB Catastrophic disturbances often provide "natural laboratories" that allow for greater understanding of ecological processes and response of natural populations. The 1980 eruption of the Mount St. Helens volcano in Washington, USA, provided a unique opportunity to test biotic effects of a large-scale stochastic disturbance, as well as the influence of post-disturbance management. Despite severe alteration of nearly 600 km 2 of habitat, coastal tailed frogs (Ascaphus truei) were found within a portion of the blast area five years after eruption. We investigated the genetic source of recolonization within the blast area and tested whether post-eruption salvage logging and subsequent tree planting influenced tailed frog movement patterns. Our results support widespread recolonization across the blast area from multiple sources, as all sites are grouped into one genetic cluster. Landscape genetic models suggest that gene flow through the unmanaged portion of the blast area is influenced only by distance between sites and the frost-free period (r(2) = 0.74). In contrast, gene flow pathways within the blast area where salvage logging and replanting occurred post-eruption are strongly limited (r(2) = 0.83) by the physiologically important variables of heat load and precipitation. These data suggest that the lack of understory and coarse wood (downed and standing dead tree boles) refugia in salvaged areas may leave frogs more susceptible to desiccation and mortality than those frogs moving through the naturally regenerated area. Simulated populations based on the landscape genetic models show an increase in the inbreeding coefficient in the managed area relative to the unmanaged blast area. In sum, we show surprising resilience of an amphibian species to a catastrophic disturbance, and we suggest that, at least for this species, naturally regenerating habitat may better maintain long-term genetic diversity of populations than actively managed habitat. C1 [Spear, Stephen F.] Orianne Soc, Clayton, GA 30525 USA. [Spear, Stephen F.; Storfer, Andrew] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Crisafulli, Charles M.] US Forest Serv, USDA, Pacific NW Res Stn, Olympia Forestry Sci Lab, Amboy, WA 98601 USA. RP Spear, SF (reprint author), Orianne Soc, 579 Highway 441 S, Clayton, GA 30525 USA. EM sspear@oriannesociety.org FU Cooperative Monitoring, Evaluation and Research Committee under the Washington Department of Natural Resources [IAA-06-047]; American Museum of Natural History; WSU College of Sciences; WSU SBS; Forest Service, Pacific Northwest Research Station; National Science Foundation (LTREB) FX We thank J. Baumsteiger, R. Dezanni, J. Evans, C. Goldberg, R. Gomulkiewicz, M. Hayes, A. McIntyre, M. Murphy, J. Sullivan, and L. Waits for insightful discussions and comments regarding sampling and analytical techniques. J. Baumsteiger, K. Chojnacki, N. Chojnacki, J. Eastman, D. Jochimsen, M. Rainey, and N. Rainey all helped with field collections. J. Baumsteiger and M. Murphy assisted with running PCR reactions for samples. Collecting permits were granted by Washington Department of Fish and Wildlife. D. Olson, D. Reed, and one anonymous reviewer provided helpful comments on a previous version of the manuscript. Genetic data for the undisturbed sites surrounding the blast area were collected as part of another project funded by the Cooperative Monitoring, Evaluation and Research Committee under the Washington Department of Natural Resources Forest Practices Adaptive Management Program (IAA-06-047) to A. Storfer. Funding for the remainder of the project was provided by the American Museum of Natural History Theodore Roosevelt Memorial Fund to S. F. Spear, WSU College of Sciences to A. Storfer, and both WSU SBS King Fellowship and WSU SBS Brislawn scholarship to S. F. Spear. C. M. Crisafulli was supported by the Forest Service, Pacific Northwest Research Station and National Science Foundation (LTREB Program). NR 72 TC 9 Z9 10 U1 1 U2 26 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD APR PY 2012 VL 22 IS 3 BP 856 EP 869 PG 14 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 932SY UT WOS:000303312000010 PM 22645816 ER PT J AU Browning, DM Duniway, MC Laliberte, AS Rango, A AF Browning, Dawn M. Duniway, Michael C. Laliberte, Andrea S. Rango, Albert TI Hierarchical analysis of vegetation dynamics over 71 years: soil-rainfall interactions in a Chihuahuan Desert ecosystem SO ECOLOGICAL APPLICATIONS LA English DT Article DE aerial photography; Chihuahuan Desert, New Mexico, USA; cross-scale linkages; ecological-state mapping; hierarchy theory; Jornada Basin LTER; object-based change detection; petrocalcic soil; Prosopis glandulosa; shrub patch dynamics; shrub proliferation; soil depth ID SEMIDESERT GRASSLAND RANGE; UNITED-STATES; SHRUB ENCROACHMENT; NEW-MEXICO; VARIABILITY; LANDSCAPES; DROUGHT; EROSION; RUNOFF; DESERTIFICATION AB Proliferation of woody plants in grasslands and savannas is a persistent problem globally. This widely observed shift from grass to shrub dominance in rangelands worldwide has been heterogeneous in space and time largely due to cross-scale interactions among soils, climate, and land-use history. Our objective was to use a hierarchical framework to evaluate the relationship between spatial patterns in soil properties and long-term shrub dynamics in the northern Chihuahuan Desert of New Mexico, USA. To meet this objective, shrub patch dynamics from 1937 to 2008 were characterized at patch and landscape scales using historical imagery and a recent digital soils map. Effects of annual precipitation on patch dynamics on two soils revealed strong correlations between shrub growth on deep sandy soils and above-average rainfall years (r = 0.671, P = 0.034) and shrub colonization and below-average rainfall years on shallow sandy soils (r = 0.705, P = 0.023). Patch-level analysis of demographic patterns revealed significant differences between shrub patches on deep and shallow sandy soils during periods of above-and below-average rainfall. Both deep and shallow sandy soils exhibited low shrub cover in 1937 (1.0% +/- 2.3% and 0.3% +/- 1.3%, respectively [mean +/- SD]) and were characterized by colonization or appearance of new patches until 1960. However, different demographic responses to the cessation of severe drought on the two soils and increased frequency of wet years after 1960 have resulted in very different endpoints. In 2008 a shrubland occupied the deep sandy soils with cover at 19.8% +/- 9.1%, while a shrub-dominated grassland occurred on the shallow sandy soils with cover at 9.3% +/- 7.2%. Present-day shrub vegetation constitutes a shifting mosaic marked by the coexistence of patches at different stages of development. Management implications of this long-term multi-scale assessment of vegetation dynamics support the notion that soil properties may constrain grassland remediation. Such efforts on sandy soils should be focused on sites characterized by near-surface water-holding capacity, as those lacking available water-holding capacity in the shallow root zone pose challenges to grass recovery and survival. C1 [Browning, Dawn M.; Duniway, Michael C.; Rango, Albert] ARS, USDA, Las Cruces, NM 88003 USA. [Laliberte, Andrea S.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Browning, DM (reprint author), ARS, USDA, POB 30003,MSC 3JER, Las Cruces, NM 88003 USA. EM dbrownin@nmsu.edu FU U.S. Department of Agriculture, Agriculture Research Service; National Science Foundation FX This research was funded by the U.S. Department of Agriculture, Agriculture Research Service, and the National Science Foundation Long-Term Ecological Research Program, Jornada Basin LTER V: Landscape Linkages in Arid and Semiarid Ecosystems. M. Mattocks assisted with the field assessment and laboratory analyses to characterize soil properties. K. Havstad, D. C. Peters, J. Karl, and two anonymous reviewers provided constructive criticisms that improved the quality of this work. NR 55 TC 13 Z9 15 U1 2 U2 63 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD APR PY 2012 VL 22 IS 3 BP 909 EP 926 PG 18 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 932SY UT WOS:000303312000014 PM 22645820 ER PT J AU O'Neil, GW Carmichael, CA Goepfert, TJ Fulton, JM Knothe, G Lau, CPL Lindell, SR Mohammady, NGE Van Mooy, BAS Reddy, CM AF O'Neil, Gregory W. Carmichael, Catherine A. Goepfert, Tyler J. Fulton, James M. Knothe, Gerhard Lau, Connie Pui Ling Lindell, Scott R. Mohammady, Nagwa G-E. Van Mooy, Benjamin A. S. Reddy, Christopher M. TI Beyond Fatty Acid Methyl Esters: Expanding the Renewable Carbon Profile with Alkenones from Isochrysis sp. SO ENERGY & FUELS LA English DT Article ID EMILIANIA-HUXLEYI; GEPHYROCAPSA-OCEANICA; LIPID EXTRACTION; BIODIESEL; TEMPERATURE; MICROALGAE; GROWTH; RECORD; CO2; FRACTIONATION AB In addition to characteristic fatty acid methyl esters (FAMEs), biodiesel produced from Isochrysis sp. contains a significant amount (14%, w/w) of predominantly C37 and C38 long-chain alkenones. These compounds are members of a class of lipids known collectively as polyunsaturated long-chain alkenones (PULCAs) that are produced by a range of other prymnesiophyte taxa. The physical properties of alkenones, such as high melting points (similar to 60 degrees C), renders the direct product unsuitable for use as a diesel fuel but, nonetheless, represents an important and as yet unexplored renewable carbon feedstock. C1 [O'Neil, Gregory W.] Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA. [Carmichael, Catherine A.; Goepfert, Tyler J.; Fulton, James M.; Van Mooy, Benjamin A. S.; Reddy, Christopher M.] Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA. [Goepfert, Tyler J.] Carl von Ossietzky Univ Oldenburg, Inst Phys, PPRE Fak 5, D-26111 Oldenburg, Germany. [Knothe, Gerhard] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Lau, Connie Pui Ling; Mohammady, Nagwa G-E.] Chinese Univ Hong Kong, Simon FS Li Marine Sci Lab, Shaitin, Hong Kong, Peoples R China. [Lindell, Scott R.] Woods Hole Oceanog Inst, Sci Aquaculture Program, Marine Biol Lab, Woods Hole, MA 02543 USA. [Mohammady, Nagwa G-E.] Univ Alexandria, Dept Bot, Fac Sci, Alexandria 21526, Egypt. RP O'Neil, GW (reprint author), Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA. EM oneil@chem.wwu.edu OI Fulton, James/0000-0001-7213-8015; Goepfert, Tyler/0000-0001-9328-8901 FU Western Washington University; M. J. Murdock Charitable Trust; Research Corporation Departmental Development Grant; Hollister Fund; Lowell Foundation at WHOI; Croucher Foundation; MBL, Rodney Berens; Charles D. Hollister Endowed Fund for Innovative Research; Hollis and Ermine Lovell Charitable Foundation; Pancha and Carl Peterson, Elizabeth and James Pickman, the Kathleen and Peter Naktenis Foundation, Frank Mittricker; Massachusetts Clean Energy Center FX The authors thank the following for their generous support of this research: Western Washington University, M. J. Murdock Charitable Trust and Research Corporation Departmental Development Grant, the Hollister Fund and Lowell Foundation at WHOI, the Croucher Foundation and Lucy B. Lemann Research Award at MBL, Rodney Berens, the Charles D. Hollister Endowed Fund for Innovative Research, the Hollis and Ermine Lovell Charitable Foundation, Pancha and Carl Peterson, Elizabeth and James Pickman, the Kathleen and Peter Naktenis Foundation, Frank Mittricker, and Massachusetts Clean Energy Center. We thank Gary Wikfors from the NOAA/NMFS Northeast Fisheries Center in Milford, CT, who provided us with the starter algal cultures. We thank Dr. John Volkman for his advice and comments during previous drafts of this manuscript. NR 67 TC 7 Z9 7 U1 3 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD APR PY 2012 VL 26 IS 4 BP 2434 EP 2441 DI 10.1021/ef3001828 PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 927QG UT WOS:000302924400051 ER PT J AU Merritt, JL Dickstein, E Johnson, RS Ward, M Balaam, RJ Harmon, CL Harmon, PF Ali, GS Palmateer, AJ Schubert, T van Bruggen, AHC AF Merritt, Joyce L. Dickstein, Ellen Johnson, Robert S. Ward, Michael Balaam, Robert J. Harmon, Carrie L. Harmon, Philip F. Ali, G. Shad Palmateer, Aaron J. Schubert, Timothy van Bruggen, Ariena H. C. TI Survey of Ornamental Nurseries in Florida Participating in the U.S.-Canadian Greenhouse Certification Program SO HORTTECHNOLOGY LA English DT Article DE Canada; emerging pests and diseases; exports; floriculture; imports; ornamental plants ID UNITED-STATES AB The purpose of this study was to evaluate the U.S.-Canadian Greenhouse Certification Program (USGCP) that was initiated in 1998. A survey consisting of 34 questions was designed and 43 out of approximate to 48 nurseries in Florida participating in the USGCP were visited. Based on the answers to the questionnaire, most of the nurseries were in compliance with the majority of USGCP requirements, growers were satisfied with the program, and there was an economic benefit to participating in the program. The main problems identified were the ambiguous wording of some of the requirements and the impracticality of keeping imported and domestic plants completely segregated. Moreover, many of the respondents did not have a written description of a pest management plan. Chi square statistical analysis showed that there was almost no difference between nursery groups in their responses to the majority of the survey questions, indicating that the USGCP is a successful program for both large and small nurseries. This quantitative assessment of the USGCP is the first assessment conducted for this program and discussed in a peer-reviewed publication. C1 [Merritt, Joyce L.; Dickstein, Ellen; Harmon, Carrie L.; Harmon, Philip F.; van Bruggen, Ariena H. C.] Univ Florida, Dept Plant Pathol, IFAS, Gainesville, FL 32611 USA. [Johnson, Robert S.; Ward, Michael] US Anim & Plant Hlth Inspect Serv, USDA, Riverdale, MD 20737 USA. [Balaam, Robert J.] US Anim & Plant Hlth Inspect Serv, USDA, Miami, FL 33158 USA. [Ali, G. Shad] Univ Florida, IFAS, Dept Plant Pathol, Midflorida Res & Educ Ctr, Apopka, FL 32703 USA. [Palmateer, Aaron J.] Univ Florida, IFAS, Trop Res & Educ Ctr, Dept Plant Pathol, Homestead, FL 33031 USA. [Schubert, Timothy] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. [van Bruggen, Ariena H. C.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. RP Merritt, JL (reprint author), Univ Florida, Dept Plant Pathol, IFAS, 1453 Fifield Hall, Gainesville, FL 32611 USA. EM joycem@ufl.edu FU U.S. Department of Agriculture, Animal and Plant Health Inspection Service FX This project was funded through the Farm Bill 2008 Section 10201(d) Cooperative Agreement Program from the U.S. Department of Agriculture, Animal and Plant Health Inspection Service. NR 25 TC 2 Z9 2 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD APR PY 2012 VL 22 IS 2 BP 169 EP 176 PG 8 WC Horticulture SC Agriculture GA 934EE UT WOS:000303425400004 ER PT J AU Essah, SYC Delgado, JA Dillon, M Sparks, R AF Essah, Samuel Y. C. Delgado, Jorge A. Dillon, Merlin Sparks, Richard TI Cover Crops Can Improve Potato Tuber Yield and Quality SO HORTTECHNOLOGY LA English DT Article DE tuber size distribution; tuber external defects ID VERTICILLIUM WILT; NITROGEN; ROTATION; N-15; CORN; RECOVERY AB There is the need to develop potato (Solanum tuberosum) cropping systems with higher yields and crop quality. Field studies were conducted with cover crops grown under limited irrigation (<8 inches) to assess the effects of certain types of cover crops on potato tuber yield and quality. On a commercial farm operation before the 2006 and 2007 potato season, mustard (Brassica sp.), canola (Brassica napus), and two cultivars of sorghum-sudangrass (Sorghum bicolor x S. sudanense) were planted. A wet fallow ground treatment where no cover crop was planted was used as a control. Before the 2008 season, barley (Hordeum vulgare), barley plus applied compost, sunflower (Helianthus annus), pea (Pisum sativum), and annual ryegrass (Lolium multiflorum) cover crops were added. The results of these 2006-08 studies showed that cover crops have the potential to increase potato tuber yield and quality, as measured by tuber size (larger tubers) and appearance (e.g., tubers with reduced defects such as cracks, knobs, and misshapes). In 2 of the 3 years, most of the cover crops, especially sorghum-sudangrass, increased yields and tuber quality. Positive results from sorghum-sudangrass suggest there is potential to harvest hay from cover crops and still obtain tuber benefits. C1 [Essah, Samuel Y. C.] Colorado State Univ, Dept Hort & Landscape Architecture, San Luis Valley Res Ctr, Center, CO 81125 USA. [Delgado, Jorge A.] ARS, USDA, Ft Collins, CO 80526 USA. [Dillon, Merlin] Colorado State Univ, Monte Vista, CO 81144 USA. [Sparks, Richard] USDA NRCS, Center, CO 81125 USA. RP Essah, SYC (reprint author), Colorado State Univ, Dept Hort & Landscape Architecture, San Luis Valley Res Ctr, Center, CO 81125 USA. EM sessah@lamar.colostate.edu NR 20 TC 3 Z9 3 U1 4 U2 23 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD APR PY 2012 VL 22 IS 2 BP 185 EP 190 PG 6 WC Horticulture SC Agriculture GA 934EE UT WOS:000303425400006 ER PT J AU Lampinen, BD Udompetaikul, V Browne, GT Metcalf, SG Stewart, WL Contador, L Negron, C Upadhyaya, SK AF Lampinen, Bruce D. Udompetaikul, Vasu Browne, Gregory T. Metcalf, Samuel G. Stewart, William L. Contador, Loreto Negron, Claudia Upadhyaya, Shrini K. TI A Mobile Platform for Measuring Canopy Photosynthetically Active Radiation Interception in Orchard Systems SO HORTTECHNOLOGY LA English DT Article DE almond; walnut; PAR interception/yield relationships; light interception; yield ID LIGHT INTERCEPTION; GROWTH; TREES AB A mobile platform was developed for measuring midday canopy photosynthetically active radiation (PAR) interception in orchards. The results presented are for almond (Prunus dulcis) and walnut (Juglans regia), but the mobile platform can be used in other orchard crops as well. The mobile platform is adjustable to accommodate orchard row spacing from 4.8 to 7.8 m and is equipped with a global positioning satellite (GPS) receiver and radar for positional assessment as well as three IR thermometers for measuring soil surface temperature. Data from the mobile platform are logged at 10 Hz and stored on a data logger. Custom software has been developed to process the data. The mobile platform was used extensively for mapping midday canopy PAR interception in almond and walnut orchards in 2009 and 2010. The mobile platform produced comparable results to those collected with a handheld light bar with the advantage of being able to cover much larger areas and compare these data to mechanically harvested yield data over the same area. For almond orchards, midday canopy PAR interception peaked at approximate to 70% at an orchard age of approximate to 12 years. For walnut orchards, midday canopy PAR interception continued to increase to approximate to 15 years of age and peaked at a level above 80%. The mobile platform was also able to follow seasonal development of midday canopy PAR interception in young and mature orchards. This technology has potential for evaluating new varieties in terms of productivity per unit PAR intercepted, in evaluating hand pruning or mechanical hedging practices in terms of impact on PAR interception/productivity as well as evaluating effectiveness of insect or disease management treatments. It also has potential as a reference point for grower self-assessment to evaluate orchard canopy development compared with other orchards of similar variety, spacing, etc. Finally, this technology could be used as ground truth referencing for remotely sensed data. C1 [Lampinen, Bruce D.; Metcalf, Samuel G.; Stewart, William L.; Contador, Loreto; Negron, Claudia] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Udompetaikul, Vasu; Upadhyaya, Shrini K.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Browne, Gregory T.] Univ Calif Davis, USDA ARS, Dept Plant Pathol, Davis, CA 95616 USA. RP Lampinen, BD (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM bdlampinen@ucdavis.edu FU Almond Board of California; California Walnut Board; USDA-ARS; USDA SCRI [CA-D-BAE-2082-OD] FX This work was supported by the Almond Board of California, the California Walnut Board, USDA-ARS Pacific Area-Wide Pest Management Program for Integrated Methyl Bromide Alternatives, and USDA SCRI Grant CA-D-BAE-2082-OD-Precision Canopy and Water Management of Specialty Crops through Sensor-Based Decision Making. NR 9 TC 14 Z9 14 U1 2 U2 20 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD APR PY 2012 VL 22 IS 2 BP 237 EP 244 PG 8 WC Horticulture SC Agriculture GA 934EE UT WOS:000303425400014 ER PT J AU Goenaga, R Jenkins, D AF Goenaga, Ricardo Jenkins, David TI Yield and Fruit Quality Traits of Mamey Sapote Cultivars Grown at Two Locations in Puerto Rico SO HORTTECHNOLOGY LA English DT Article DE soluble solids concentration; Pouteria sapota ID HOST STATUS; TEPHRITIDAE; DIPTERA; FLY AB The demand for tropical fruits has increased significantly during the last decade as consumers seek healthy and more diverse food products. There is a lack of formal experimentation to determine yield performance and fruit quality traits of mamey sapote (Pouteria sapota) cultivars. Six mamey sapote cultivars (Copan, Magana, Mayapan, Pace, Pantin, Tazumal) grown on Ultisol and Oxisol soils were evaluated for 5 years at Corozal and Isabela, PR, respectively. There was a significant difference in the number and weight of fruit per hectare between locations, averaging 25,929 fruit/ha and 16,527 kg.ha(-1) at Corozal and 17,887 fruit/ha and 11,920 kg.ha(-1) at Isabela. 'Tazumal' had the highest 5-year mean number and weight of fruit per hectare, but fruit of this cultivar was very small and contained several seeds, which could reduce its marketability. At Corozal, cultivars Tazumal and Magana had significantly higher fruit yield per hectare than the rest of the cultivars, whereas 'Magana', 'Tazumal', and 'Pantin' had the highest fruit yield at Isabela. At both locations, 'Pantin' had relatively high yield, above-average soluble solids concentration values, and adequate fruit size and weight for domestic and export markets (650-900 g), making this cultivar suitable for planting at various agroenvironments typical of the humid tropics. C1 [Goenaga, Ricardo; Jenkins, David] USDA ARS, Trop Agr Res Stn, Mayaguez, PR 00680 USA. RP Goenaga, R (reprint author), USDA ARS, Trop Agr Res Stn, 2200 PA Campos Ave,Suite 201, Mayaguez, PR 00680 USA. EM Ricardo.Goenaga@ars.usda.gov NR 23 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD APR PY 2012 VL 22 IS 2 BP 263 EP 267 PG 5 WC Horticulture SC Agriculture GA 934EE UT WOS:000303425400018 ER PT J AU Bennett, MM Petersen, K Yocum, G Rinehart, J Greenlee, K AF Bennett, M. M. Petersen, K. Yocum, G. Rinehart, J. Greenlee, K. TI The effects of extended diapause duration on the metabolic rate and critical PO2 of the alfalfa leafcutting bee, Megachilee rotundata SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2012 CL Charleston, SC SP Soc Integrat & Comparat Biol (SICB) C1 N Dakota State Univ, Fargo, ND USA. USDA, Washington, DC USA. EM meghan.bennett@ndsu.edu NR 0 TC 0 Z9 0 U1 2 U2 6 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD APR PY 2012 VL 52 SU 1 BP E210 EP E210 PG 1 WC Zoology SC Zoology GA 930TV UT WOS:000303165001305 ER PT J AU Shields, VDC Sanford, JL Otalora-Luna, F Dickens, JC AF Shields, Vonnie D. C. Sanford, Jillian L. Otalora-Luna, Fernando Dickens, Joseph C. TI Do You See What I See?: Visual Orientation Behavior of Colorado Potato Beetle Larvae to Emissive Colors SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2012 CL Charleston, SC SP Soc Integrat & Comparat Biol (SICB) C1 [Shields, Vonnie D. C.; Sanford, Jillian L.; Otalora-Luna, Fernando; Dickens, Joseph C.] Towson Univ, Inst Venezolano Invest Cient, USDA, Beltsville Agr Res Ctr, Towson, MD USA. EM vshields@towson.edu NR 0 TC 0 Z9 0 U1 0 U2 3 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD APR PY 2012 VL 52 SU 1 BP E159 EP E159 PG 1 WC Zoology SC Zoology GA 930TV UT WOS:000303165001104 ER PT J AU Somjee, U Ablard, K Crespi, B Schaefer, PW Gries, G AF Somjee, Ummat Ablard, Kelly Crespi, Bernard Schaefer, Paul W. Gries, Gerhard TI Sex ratio adjustment in a solitary parasitoid wasp SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2012 CL Charleston, SC SP Soc Integrat & Comparat Biol (SICB) C1 Simon Fraser Univ, Vancouver, BC, Canada. USDA, Newark, DE USA. EM ummat.s@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD APR PY 2012 VL 52 SU 1 BP E164 EP E164 PG 1 WC Zoology SC Zoology GA 930TV UT WOS:000303165001122 ER PT J AU Srygley, RB Jaronski, ST AF Srygley, R. B. Jaronski, S. T. TI Adaptive melanism and immunity to fungal infection in the migratory grasshopper SO INTEGRATIVE AND COMPARATIVE BIOLOGY LA English DT Meeting Abstract CT Annual Meeting of the Society-for-Integrative-and-Comparative-Biology (SICB) CY JAN 03-07, 2012 CL Charleston, SC SP Soc Integrat & Comparat Biol (SICB) C1 [Srygley, R. B.; Jaronski, S. T.] ARS, USDA, Washington, DC USA. EM robert.srygley@ars.usda.gov RI Jaronski, Stefan/E-8507-2012 OI Jaronski, Stefan/0000-0002-7789-0406 NR 0 TC 0 Z9 0 U1 0 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1540-7063 J9 INTEGR COMP BIOL JI Integr. Comp. Biol. PD APR PY 2012 VL 52 SU 1 BP E166 EP E166 PG 1 WC Zoology SC Zoology GA 930TV UT WOS:000303165001132 ER PT J AU Johnston, CA Tyler, C Stansberry, SA Moreno, JP Foreyt, JP AF Johnston, Craig A. Tyler, Chermaine Stansberry, Sandra A. Moreno, Jennette P. Foreyt, John P. TI Brief report: Gum chewing affects standardized math scores in adolescents SO JOURNAL OF ADOLESCENCE LA English DT Article DE Gum chewing; Academic performance; Children & adolescents ID ANXIETY; CHILDREN; MEMORY AB Gum chewing has been shown to improve cognitive performance in adults; however, gum chewing has not been evaluated in children. This study examined the effects of gum chewing on standardized test scores and class grades of eighth grade math students. Math classes were randomized to a gum chewing (GC) condition that provided students with gum during class and testing, or a control condition with no gum (NGC). Participants included 108 students. The math sections of the Texas Assessment of Knowledge and Skills (TANS) and the Woodcock Johnson Ill Tests of Achievement (WJ-III), and math class grades were used to assess academic performance. Students in the gum chewing condition improved standardized test scores and maintained higher grades in math class compared to those in the no-gum chewing condition. These results are encouraging as gum chewing may be a cost-effective and easily implemented method to increase student performance. Trial Registration: NCT00792116. (C) 2011 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved. C1 [Johnston, Craig A.; Tyler, Chermaine; Stansberry, Sandra A.; Moreno, Jennette P.; Foreyt, John P.] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat Nutr,Baylor Coll Med, Houston, TX 77030 USA. [Foreyt, John P.] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. RP Johnston, CA (reprint author), ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat Nutr,Baylor Coll Med, 1100 Bates, Houston, TX 77030 USA. EM caj@bcm.edu NR 16 TC 0 Z9 0 U1 3 U2 22 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1971 J9 J ADOLESCENCE JI J. Adolesc. PD APR PY 2012 VL 35 IS 2 BP 455 EP 459 DI 10.1016/j.adolescence.2011.04.003 PG 5 WC Psychology, Developmental SC Psychology GA 929YF UT WOS:000303100900022 PM 21620465 ER PT J AU Neven, LG AF Neven, Lisa G. TI Fate of Codling Moth (Lepidoptera: Tortricidae) in Harvested Apples Held Under Short Photoperiod SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE codling moth; Cydia pomonella; apple; quarantine; diapause ID POMONELLA L LEPIDOPTERA; CYDIA-POMONELLA; LASPEYRESIA-POMONELLA; COLD-STORAGE; TEMPERATURE; DIAPAUSE; LARVAE AB Codling moth, Cydia pomonella L., is a cosmopolitan pest of pome and stone fruits. It has been identified as a quarantine pest of concern in a number of countries where it is not known to occur, most of them tropical or subtropical countries. Although considerable work has been done on the basic biology and physiology of this temperate pest, little is known on its potential to develop and establish in tropical environments with short photoperiods and few to no days below 10 degrees C. Apples were harvested over three field seasons (2007-2009) from unmanaged orchards in central Washington State and subjected to simulated commercial cold storage at 1.1 +/- 2 degrees C for up to 119 d. After cold storage, infested fruits were held at 20 degrees C under a 12:12 L:D photoperiod for up to 6 mo. Over the entire experiment only 27% of the larvae collected exited the fruit and cocooned. Of those 27%, only 1.06% of larvae held under a 12:12 L:D photoperiod successfully emerged as moths. No moths emerged when host fruit would be available in a representative importing country in the tropics over the 3yr of testing. These results indicate that codling moth in apples from the Pacific Northwest pose little threat of surviving and establishing in tropical regions where daylength is insufficient to break diapause and the chilling requirement is not met. C1 ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Neven, LG (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM lisa.neven@ars.usda.gov FU Washington Tree Fruit Research Commission FX I would like to thank the Washington Tree Fruit Research Commission for the funding to support this research. I would also like to thank Mike Willett of the Northwest Horticultural Council for assisting in the development of this project and providing shipment data of apples to Taiwan. I thank Peter Follett for his reviews of this manuscript and helpful insights for improvements. I would also like to acknowledge the assistance of Mike Doerr of Washington State University, Tree Fruit Research Center in Wenatchee, WA, for securing codling moth infested apples. This project would not have been possible without the technical assistance of Michele Watkins, Anne Kenny Chapman, Dan Hallarer, Blaine Heilman, and Fred Lopez USDA ARS, Wapato, WA. NR 26 TC 4 Z9 4 U1 2 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 297 EP 303 DI 10.1603/EC11242 PG 7 WC Entomology SC Entomology GA 925TL UT WOS:000302784300001 PM 22606796 ER PT J AU Danka, RG De Guzman, LI Rinderer, TE Sylvester, HA Wagener, CM Bourgeois, AL Harris, JW Villa, JD AF Danka, Robert G. De Guzman, Lilia I. Rinderer, Thomas E. Sylvester, H. Allen Wagener, Christine M. Bourgeois, A. Lelania Harris, Jeffrey W. Villa, Jose D. TI Functionality of Varroa-Resistant Honey Bees (Hymenoptera: Apidae) When Used in Migratory Beekeeping for Crop Pollination SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Varroa destructor; genetic resistance; migratory beekeeping; crop pollination; Apis mellifera ID DESTRUCTOR; MESOSTIGMATA; POPULATIONS; FLUVALINATE; COLONIES; AMITRAZ; STOCKS; ARS AB Two types of honey bees, Apis mellifera L. (Hymenoptera: Apidae), bred for resistance to Varroa destructor Anderson & Trueman were evaluated for performance when used in migratory crop pollination. Colonies of Russian honey bees (RHB) and outcrossed bees with Varroa-sensitive hygiene (VSH) were managed without miticide treatments and compared with colonies of Italian honey bees that served as controls. Control colonies were managed as groups which either were treated twice each year against V. destructor (CT) or kept untreated (CU). Totals of 240 and 247 colonies were established initially for trials in 2008 and 2009, respectively. RHB and VSH colonies generally had adult and brood populations similar to those of the standard CT group regarding pollination requirements. For pollination of almonds [Prunus dulcis (Mill.) D. A. Webb] in February, percentages of colonies meeting the required six or more frames of adult bees were 57% (VSH), 56% (CT), 39% (RHB), and 34% (CU). RHB are known to have small colonies in early spring, but this can be overcome with appropriate feeding. For later pollination requirements in May to July, 94-100% of colonies in the four groups met pollination size requirements for apples (Malus domestica Borkh.), cranberries (Vaccinium macrocarpon Aiton), and lowbush blueberries (Vaccinium angustifolium Aiton). Infestations with V. destructor usually were lowest in CT colonies and tended to be lower in VSH colonies than in RHB and CU colonies. This study demonstrates that bees with the VSH trait and pure RHB offer alternatives for beekeepers to use for commercial crop pollination while reducing reliance on miticides. The high frequency of queen loss (only approximately one fourth of original queens survived each year) suggests that frequent requeening is necessary to maintain desired genetics. C1 [Danka, Robert G.; De Guzman, Lilia I.; Rinderer, Thomas E.; Sylvester, H. Allen; Wagener, Christine M.; Bourgeois, A. Lelania; Harris, Jeffrey W.; Villa, Jose D.] ARS, USDA, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. RP Danka, RG (reprint author), ARS, USDA, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM bob.danka@ars.usda.gov FU USDA-ARS FX We thank Merrimack Valley Apiaries and Evergreen Honey Company (Andy, Crystal, Wesley, and Glenn Card and especially Joe Sanroma) for valuable beekeeping collaboration. Technical assistance was provided by USDA-ARS personnel Lorraine Beaman, Garrett Dodds, David Dodge, Amanda Frake, Sandra Hineman, Stephanie Johnson, Victor Rainey, J. Anthony Stelzer, Joshua Wales, Rachel Watts, and Daniel Winfrey. Debbie Boykin and Amanda Frake (USDA-ARS) gave advice on statistical analyses. Funding was provided by the USDA-ARS Areawide Program "Improving Honey Bee Health, Survivorship and Pollination Availability." NR 22 TC 8 Z9 8 U1 5 U2 47 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 313 EP 321 DI 10.1603/EC11286 PG 9 WC Entomology SC Entomology GA 925TL UT WOS:000302784300003 PM 22606798 ER PT J AU Mangan, RL Moreno, AT AF Mangan, Robert L. Moreno, Aleena Tarshis TI Host Status of Meyer and Eureka Lemons for Anastrepha ludens SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tephritidae; Rutaceae; Mexican fruit fly; Citrus limon ID TEPHRITIDAE; DIPTERA; MEXICO; SERPENTINA; PLANT AB Host status for Mexican fruit fly (Anastrepha ludens (Loew)) was examined under laboratory conditions in cage infested Eureka and Meyer lemons. Our approach was to allow females to oviposit on the two cultivars in separate laboratory cages with aluminum foil covering to restrict the areas where females had access to fruit surface. Fruit of each cultivar were placed in covered trays for incubations and at approximately weekly intervals, fruit were removed, dissected, and live and dead eggs and larvae tabulated in each tissue of the fruit. Infestation and survival were tabulated and analyzed for the effects of harvest date, fruit color and brix indices, postoviposition period, and cultivar. Infestation rate, determined by counts of total eggs and larvae was significantly higher in Meyer lemons. In both cultivars, females deposited eggs into both albedo and pulp tissue but not into flavedo. Both cultivars showed high resistance (>90% mortality) to egg and first instars survival in albedo and pulp. Second and third instars surviving in the pulp had high survival rates (>60%) in both cultivars in fruit dissected at weeks 2-4 after infestation. Total adults produced were slightly higher, and total second and third stage larvae were also higher for Meyer lemons. Numbers of adults and total second and third stage larvae increased in Eureka lemons in more mature fruit, but the higher numbers in Meyer lemons were not associated with fruit maturity, at time of infestation. Numbers of second and third stage larvae were significantly correlated with some fruit color indices in Eureka but not in Meyer lemons. Application of these results to quarantine risk analysis is discussed. C1 [Mangan, Robert L.; Moreno, Aleena Tarshis] ARS, USDA, Crop Qual & Fruit Insects Res Unit, Kika Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. RP Mangan, RL (reprint author), ARS, USDA, Crop Qual & Fruit Insects Res Unit, Kika Garza Subtrop Agr Res Ctr, 2413 E Highway 83, Weslaco, TX 78596 USA. EM robert.mangan@ars.usda.gov NR 26 TC 2 Z9 2 U1 0 U2 3 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 363 EP 370 DI 10.1603/EC11241 PG 8 WC Entomology SC Entomology GA 925TL UT WOS:000302784300009 PM 22606804 ER PT J AU Graham, EE Poland, TM AF Graham, Elizabeth E. Poland, Therese M. TI Efficacy of Fluon Conditioning for Capturing Cerambycid Beetles in Different Trap Designs and Persistence on Panel Traps Over Time SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE wood-boring insect; Fluon; Cerambycidae; pheromone trap ID TETROPIUM-FUSCUM F; COLEOPTERA-CERAMBYCIDAE; AGGREGATION PHEROMONES; SCUTELLATUS COLEOPTERA; MULTIPLE-FUNNEL; ATTRACTANTS; INSECTS; CATCHES AB Fluon PTFE is a fluoropolymer dispersion applied as a surface conditioner to cross-vane panel traps to enhance trap efficiency for cerambycid beetles. We describe the results of three experiments to further optimize cerambycid traps of different designs and to test the effect of Fluon over time. We tested Fluon with Lindgren funnel and panel traps fitted with either wet or dry collection cups on catches of cerambycid beetles and how the effect of Fluon on panel traps persisted. Fluon-treated funnel traps with wet collection cups captured approximate to 6x more beetles than the untreated funnel traps with wet collection cups. Untreated funnel traps with dry collection cups did not capture any beetles; however, Fluon-treated funnel traps with dry collection cups captured an average of four beetles per trap. Fluon-treated panel traps with wet collection cups captured approximate to 9x more beetles than untreated panel traps with wet collection cups. Fluon-treated panel traps with dry collection cups captured approximate to 11x more beetles than untreated panel traps with dry collection cups. The effect of Fluon on capturing cerambycid beetles did not decline after use in one or two field seasons. There was no significant difference in the number of beetles captured in freshly treated panel traps compared with traps that had been used for 1 or 2yr. Fluon-treated traps captured nine species that were not captured in untreated traps. Conditioning both Lindgren funnel and panel traps with Fluon enhances the efficacy and sensitivity of traps deployed to detect exotic cerambycid species, or for monitoring threatened species at low population densities. C1 [Graham, Elizabeth E.] Michigan State Univ, Dept Entomol, E Lansing, MI 48823 USA. [Poland, Therese M.] US Forest Serv, USDA, E Lansing, MI 48823 USA. RP Graham, EE (reprint author), Michigan State Univ, Dept Entomol, E Lansing, MI 48823 USA. EM graha139@msu.edu FU USDA Forest Service, Northeastern Area FX We thank Tina Ciaramitaro and Tom Baweja for their assistance in the field, Greg Kowalewski for access to the Kellogg Experimental Forest, Lawrence Hanks for his input on the study design, Jocelyn Millar for providing the 3R* pheromone, Thomas Rachman and Paige Plebanek for their help sorting trap catches, and Ann Ray and Robert Mitchell for their helpful comments on an earlier version of the manuscript. This study was supported by the USDA Forest Service, Northeastern Area, Special Technology Development Program. NR 22 TC 14 Z9 14 U1 0 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 395 EP 401 DI 10.1603/EC11432 PG 7 WC Entomology SC Entomology GA 925TL UT WOS:000302784300014 PM 22606809 ER PT J AU Niu, GD Pollock, HS Lawrance, A Siegel, JP Berenbaum, MR AF Niu, Guodong Pollock, Henry S. Lawrance, Allen Siegel, Joel P. Berenbaum, May R. TI Effects of a Naturally Occurring and a Synthetic Synergist on Toxicity of Three Insecticides and a Phytochemical to Navel Orangeworm (Lepidoptera: Pyralidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Amyelois transitella; cytochrome P450 monooxygenase; synergist; myristicin; insecticide ID AFLATOXIN CONTAMINATION; ASPERGILLUS-PARASITICUS; AMYELOIS-TRANSITELLA; MYRISTICIN; XANTHOTOXIN; METABOLISM; COMPONENTS; RESISTANCE; INHIBITORS; DILLAPIOL AB The navel orangeworm, Amyelois transitella (Walker) (Lepidoptera: Pyralidae), is the most destructive lepidopteran pest of almonds [Prunus dulcis (Mill.) D.A. Webb] and pistachios (Pistacia vera L.) in California and is a serious problem in figs (Ficus carica L.) and walnuts (Juglans spp.). In addition to direct damage, larval feeding leaves nuts vulnerable to infection by Aspergillus spp., fungi that produce toxic aflatoxins. A potentially safe and sustainable approach for managing navel orangeworm in orchards may be to use natural essential oil synergists to interfere with this insect's ability to detoxify insecticides and phytochemicals. We tested the effects of a naturally occurring plant-derived chemical, myristicin, and a synthetic inhibitor of cytochrome P450 monooxygenases (P450s), piperonyl butoxide, on the toxicity of three insecticides (alpha-cypermethrin, tau-fluvalinate, and methoxyfenozide [Intrepid]) and a phytochemical (xanthotoxin) to A. transitella. Piperonyl butoxide significantly synergized alpha-cypermethrin and tau-fluvalinate, whereas myristicin synergized only alpha-cypermethrin. Piperonyl butoxide synergized the toxicity of xanthotoxin as early as 72 h after exposure, whereas myristicin synergized xanthotoxin after 120 h. In view of these findings and the limited availability of environmentally safe synthetic insecticides for sustainable management, particularly in organic orchards, myristicin is a potential field treatment in combination with insecticides to reduce both navel orangeworm survival and aflatoxin contamination of nuts. In addition, this study demonstrates that in A. transitella the insect growth regulator methoxyfenozide is not detoxified by P450s. C1 [Niu, Guodong; Pollock, Henry S.; Lawrance, Allen; Berenbaum, May R.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Siegel, Joel P.] ARS, USDA, SJVASC, Parlier, CA 93648 USA. RP Berenbaum, MR (reprint author), Univ Illinois, Dept Entomol, 320 Morrill Hall, Urbana, IL 61801 USA. EM maybe@illinois.edu RI Niu, Guodong/E-5846-2012 OI Niu, Guodong/0000-0002-1406-6698 FU Almond Board of California; Pistachio Board of California FX We thank Mary A. Schuler for valuable discussion and editorial assistance and three anonymous reviewers for thorough evaluation of the manuscript and helpful suggestions. We also thank the Almond Board of California and the Pistachio Board of California for financial support and encouragement. NR 39 TC 11 Z9 11 U1 0 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 410 EP 417 DI 10.1603/EC10194 PG 8 WC Entomology SC Entomology GA 925TL UT WOS:000302784300016 PM 22606811 ER PT J AU Nagoshi, RN Murua, MG Hay-Roe, M Juarez, ML Willink, E Meagher, RL AF Nagoshi, Rodney N. Gabriela Murua, M. Hay-Roe, Mirian Laura Juarez, M. Willink, Eduardo Meagher, Robert L. TI Genetic Characterization of Fall Armyworm (Lepidoptera: Noctuidae) Host Strains in Argentina SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Spodoptera frugiperda; haplotype; triose phosphate isomerase; cytochrome oxidase I ID SPODOPTERA-FRUGIPERDA LEPIDOPTERA; FRAGMENT-LENGTH-POLYMORPHISMS; MATING COMPATIBILITY; MITOCHONDRIAL-DNA; UNITED-STATES; I GENE; POPULATIONS; FLORIDA; BRAZIL; TEXAS AB Fall armyworm is a major economic pest throughout the Western Hemisphere. Previous studies of populations in the southern United States, Brazil, and the Caribbean demonstrated the existence of two morphologically identical but genetically distinct host strains that can only be distinguished using genetic markers, including polymorphisms in the mitochondrial Cytochrome Oxidase I (COI) gene and in the Z-chromosome linked Triose phosphate isomerase (Tpi) gene. The strains differ in some physiological and behavioral characteristics, most notably their preference for different plant hosts, but are capable of hybridizing in the laboratory and in the field. These traits suggest that the strains are in the process of divergence, which may or may not be hemispheric in scope. The objective of this study was to determine whether the two strains are present in Argentina. It was found that the strain-diagnostic haplotypes of the COI and Tpi genes subdivided the Argentina population into two major groups. Each group displayed biases in their distribution among different host plants that were generally consistent with expected strain behavior. The overall results indicated that Argentina fall armyworm exhibit similar genetics and behavior to populations in the rest of the hemisphere. In addition, the Argentina populations had comparable haplotype frequencies to those from Brazil and Texas, consistent with possible interactions with these fall armyworm groups, but appeared to have had minimal exchanges with those from Puerto Rico or Florida. C1 [Nagoshi, Rodney N.; Hay-Roe, Mirian; Meagher, Robert L.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32604 USA. [Gabriela Murua, M.; Laura Juarez, M.; Willink, Eduardo] Estn Expt Agroind Obispo Colombres, Secc Zool Agr, San Miguel De Tucuman, Argentina. [Gabriela Murua, M.] Consejo Nacl Invest Cient & Tecn, Secc Zool Agr Estac Expt Agroind Obispo Colombres, RA-4101 San Miguel De Tucuman, Argentina. RP Nagoshi, RN (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32604 USA. EM rodney.nagoshi@ars.usda.gov NR 44 TC 10 Z9 10 U1 0 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 418 EP 428 DI 10.1603/EC11332 PG 11 WC Entomology SC Entomology GA 925TL UT WOS:000302784300017 PM 22606812 ER PT J AU Crook, DJ Khrimian, A Cosse, A Fraser, I Mastro, VC AF Crook, Damon J. Khrimian, Ashot Cosse, Allard Fraser, Ivich Mastro, Victor C. TI Influence of Trap Color and Host Volatiles on Capture of the Emerald Ash Borer (Coleoptera: Buprestidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Agrilus planipennis; (3Z)-hexenol; manuka oil; phoebe oil; dark green trap ID AGRILUS-PLANIPENNIS COLEOPTERA; MELOLONTHA-HIPPOCASTANI; PLANT VOLATILES; PRISM TRAPS; (Z)-3-HEXENOL; ATTRACTION; PLACEMENT; RESPONSES; FEMALES; REGIONS AB Field trapping assays were conducted in 2009 and 2010 throughout western Michigan, to evaluate lures for adult emerald ash borer, A. planipennis Fairmaire (Coleoptera: Buprestidae). Several ash tree volatiles were tested on purple prism traps in 2009, and a dark green prism trap in 2010. In 2009, six bark oil distillate lure treatments were tested against manuka oil lures (used in 2008 by USDA APHIS PPQ emerald ash borer cooperative program). Purple traps baited with 80/20 (manuka/phoebe oil) significantly increased beetle catch compared with traps baited with manuka oil alone. In 2010 we monitored emerald ash borer attraction to dark green traps baited with six lure combinations of 80/20 (manuka/phoebe), manuka oil, and (3Z)-hexenol. Traps baited with manuka oil and (3Z)-hexenol caught significantly more male and total count insects than traps baited with manuka oil alone. Traps baited with manuka oil and (3Z)-hexenol did not catch more beetles when compared with traps baited with (3Z)-hexenol alone. When compared with unbaited green traps our results show that (3Z)-hexenol improved male catch significantly in only one of three field experiments using dark green traps. Dark green traps caught a high number of A. planipennis when unbaited while (3Z)-hexenol was seen to have a minimal (nonsignificant) trap catch effect at several different release rates. We hypothesize that the previously reported kairomonal attractancy of (3Z)-hexenol (for males) on light green traps is not as obvious here because of improved male attractancy to the darker green trap. C1 [Crook, Damon J.; Mastro, Victor C.] APHIS, USDA, PPQ, Buzzards Bay, MA 02542 USA. [Khrimian, Ashot] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Cosse, Allard] ARS, USDA, Nat Ctr Agr Utilizat Res NCAUR, Peoria, IL 61604 USA. [Fraser, Ivich] APHIS, USDA, PPQ, Brighton, MI 48116 USA. RP Crook, DJ (reprint author), APHIS, USDA, PPQ, 1398 W Truck Rd, Buzzards Bay, MA 02542 USA. EM damon.j.crook@aphis.usda.gov FU USDA APHIS PPQ FX This research was funded by the USDA APHIS PPQ Emerald Ash Borer program. We thank the staff of the USDA-APHIS-PPQ CPHST EAB laboratory (Brighton, MI): Michael Rietz, Nicole Smith, Janna Daimler, Ariel Andrews, Bethany Boegler, Kevin Donovan, Tony Kern, Joe Rogers, Grace O'Connor, Kelly Schmiermund, Ben Sorensen, Carissa Vanderschaaf, and Joshua Warlick for fieldwork assistance. We thank Joe Francese, Al Sawyer, Dave Lance (APHIS, USDA), and Peter Silk (Canadian Forest Service, New Brunswick, Canada) for reviewing an earlier draft of the manuscript. NR 28 TC 19 Z9 19 U1 2 U2 31 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 429 EP 437 DI 10.1603/EC11204 PG 9 WC Entomology SC Entomology GA 925TL UT WOS:000302784300018 PM 22606813 ER PT J AU Koch, FH Yemshanov, D Magarey, RD Smith, WD AF Koch, Frank H. Yemshanov, Denys Magarey, Roger D. Smith, William D. TI Dispersal of Invasive Forest Insects via Recreational Firewood: A Quantitative Analysis SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biological invasion; firewood; invasive forest pest; human-mediated dispersal; long-distance dispersal ID LONG-DISTANCE DISPERSAL; AGRILUS-PLANIPENNIS COLEOPTERA; EMERALD ASH BORER; BIOLOGICAL INVASIONS; PLANT MIGRATION; SPREAD; CERAMBYCIDAE; BUPRESTIDAE; DYNAMICS; BEETLE AB Recreational travel is a recognized vector for the spread of invasive species in North America. However, there has been little quantitative analysis of the risks posed by such travel and the associated transport of firewood. In this study, we analyzed the risk of forest insect spread with firewood and estimated related dispersal parameters for application in geographically explicit invasion models. Our primary data source was the U.S. National Recreation Reservation Service database, which records camper reservations at >2,500 locations nationwide. For >7 million individual reservations made between 2004 and 2009 (including visits from Canada), we calculated the distance between visitor home address and campground location. We constructed an empirical dispersal kernel (i.e., the probability distribution of the travel distances) from these "origin-destination" data, and then fitted the data with various theoretical distributions. We found the data to be strongly leptokurtic (fat-tailed) and fairly well fit by the unbounded Johnson and lognormal distributions. Most campers (approximate to 53%) traveled <100 km, but approximate to 10% traveled >500 km (and as far as 5,500 km). Additionally, we examined the impact of geographic region, specific destinations (major national parks), and specific origin locations (major cities) on the shape of the dispersal kernel, and found that mixture distributions (i.e., theoretical distribution functions composed of multiple univariate distributions) may fit better in some circumstances. Although only a limited amount of all transported firewood is likely to be infested by forest insects, this still represents a considerable increase in dispersal potential beyond the insects' natural spread capabilities. C1 [Koch, Frank H.; Smith, William D.] US Forest Serv, USDA, So Res Stn, Eastern Forest Environm Threat Assessment Ctr, Res Triangle Pk, NC 27709 USA. [Yemshanov, Denys] Nat Resources Canada, Canadian Forest Serv, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2E5, Canada. [Magarey, Roger D.] N Carolina State Univ, Ctr Integrated Pest Management, USDA,Anim & Plant Hlth Inspect Serv, Plant Protect & Quarantine Div,Ctr Plant Hlth Sci, Raleigh, NC 27606 USA. RP Koch, FH (reprint author), US Forest Serv, USDA, So Res Stn, Eastern Forest Environm Threat Assessment Ctr, 3041 Cornwallis Rd, Res Triangle Pk, NC 27709 USA. EM fhkoch@fs.fed.us OI Koch, Frank/0000-0002-3750-4507 FU U.S. Department of Agriculture, Forest Service, Southern Research Station, Asheville, NC [10-JV-11330146-064]; North Carolina State University [10-JV-11330146-064] FX We thank Judith Pasek, David Kowalski, and Daniel Borchert (USDA-APHIS) for providing the data used in this study. We also thank Kurt Riitters and John Coulston (USDA Forest Service), as well as two anonymous reviewers, for their helpful comments. Frank Koch's initial work on the study was supported by Research Joint Venture Agreement 10-JV-11330146-064 between the U.S. Department of Agriculture, Forest Service, Southern Research Station, Asheville, NC, and North Carolina State University. NR 52 TC 9 Z9 9 U1 4 U2 42 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 438 EP 450 DI 10.1603/EC11270 PG 13 WC Entomology SC Entomology GA 925TL UT WOS:000302784300019 PM 22606814 ER PT J AU Strand, TM Ross, DW Thistle, HW Ragenovich, IR Guerra, IM Lamb, BK AF Strand, Tara M. Ross, Darrell W. Thistle, Harold W. Ragenovich, Iral R. Guerra, Ivonne Matos Lamb, Brian K. TI Predicting Dendroctonus pseudotsugae (Coleoptera: Curculionidae) Antiaggregation Pheromone Concentrations Using an Instantaneous Puff Dispersion Model SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Douglas-fir beetle; Scolytinae; MCH; 3-methylcylcohex-2-en-1-one; pheromone plume ID DOUGLAS-FIR BEETLE; HIGH-RISK STANDS; INFESTATION; SCOLYTIDAE; 3-METHYLCYCLOHEX-2-EN-1-ONE; DISPENSERS; HOPKINS; FORESTS AB An instantaneous puff dispersion model was used to assess concentration fields of the Douglas-fir beetle, Dendroctonus pseudotsugae Hopkins, antiaggregation pheromone, 3-methylcyclohex-2-en-1-one (MCH), within a 1-ha circular plot. Several combinations of MCH release rate and releaser spacing were modeled to theoretically analyze optimal deployment strategies. The combinations of MCH release rate and releaser spacing used in the modeling exercise were based on results of previous field studies of treatment efficacy. Analyses of model results suggest that a release rate up to six times the initial standard, at a correspondingly wider spacing to keep the total amount of pheromone dispersed per unit area constant, may be effective at preventing Douglas-fir beetle infestation. The model outputs also provide a visual representation of pheromone dispersion patterns that can occur after deployment of release devices in the field. These results will help researchers and practitioners design more effective deployment strategies. C1 [Strand, Tara M.] Scion Res, Rotorua 3046, New Zealand. [Strand, Tara M.] US Forest Serv, AirFire Res Team, Pacific NW Lab, USDA, Seattle, WA 98103 USA. [Ross, Darrell W.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Thistle, Harold W.] US Forest Serv, Forest Hlth Technol Enterprise Team, USDA, Morgantown, WV 26505 USA. [Ragenovich, Iral R.] US Forest Serv, USDA, Portland, OR 97208 USA. [Guerra, Ivonne Matos] Univ Puerto Rico, Mayaguez, PR 00681 USA. [Lamb, Brian K.] Washington State Univ, Lab Atmospher Res, Pullman, WA 99164 USA. RP Strand, TM (reprint author), Scion Res, 49 Sala St, Rotorua 3046, New Zealand. EM Tara.Strand@scionresearch.com FU USDA Forest Service, Forest Health Protection, Forest Health Technology Enterprise Team, Morgantown, WV; American Recovery and Reinvestment Act FX We are grateful for the many contributions of Gary Daterman (deceased) to the field studies upon which the current work is based. Funding for this work was provided through the USDA Forest Service, Forest Health Protection, Forest Health Technology Enterprise Team, Morgantown, WV. The American Recovery and Reinvestment Act provided funding for the Hispanic Association of Colleges and Universities internship under which Matos Guerra was able to work on this project. NR 27 TC 2 Z9 2 U1 0 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 451 EP 460 DI 10.1603/EC11282 PG 10 WC Entomology SC Entomology GA 925TL UT WOS:000302784300020 PM 22606815 ER PT J AU Mayfield, AE Hanula, JL AF Mayfield, A. E., III Hanula, J. L. TI Effect of Tree Species and End Seal on Attractiveness and Utility of Cut Bolts to the Redbay Ambrosia Beetle and Granulate Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Scolytinae; exotic species; attraction; emergence; laurel wilt ID XYLEBORUS-GLABRATUS COLEOPTERA; SOUTHEASTERN UNITED-STATES; LAUREL WILT; RAFFAELEA-LAURICOLA; 1ST REPORT; FLORIDA; LAURACEAE; AVOCADO; SYMBIONT; PATHOGEN AB The redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is a non-native invasive pest and vector of the fungus that causes laurel wilt disease in certain trees of the family Lauraceae. This study assessed the relative attractiveness and suitability of cut bolts of several tree species to X. glabratus. In 2009, female X. glabratus were equally attracted to traps baited with swampbay (Persea palustris (Rafinesque) Sargent) and camphortree (Cinnamomum camphora (L.) J. Presl), which were more attractive than avocado (Persea americana Miller), lancewood (Ocotea coriacea (Swartz) Britton), and sweetbay (Magnolia virginiana L.). These species were more attractive than loblolly bay (Gordonia lasianthus (L.) J. Ellis). X. glabratus entrance hole density and emergence from caged bolts were highest on swampbay and camphortree. In 2010, swampbay was significantly more attractive to X. glabratus than sassafras (Sassafras albidum (Nuttall) Nees), yellow poplar (Liriodendron tulipifera L.), and eastern redbud (Cercis canadensis L.). Sassafras bolts end sealed with a liquid wax-and-water emulsion were more attractive to X. glabratus than end-sealed bolts of yellow poplar and redbud. Relative to unsealed bolts, end seal decreased X. glabratus entrance hole density on swampbay and decreased granulate ambrosia beetle (Xylosandrus crassiusculus (Motschulsky)) trap catch, entrance hole density, and adult emergence from swampbay. X. crassiusculus was not attracted to sassafras, yellow poplar, and redbud and was not more attracted to manuka oil than to unbaited traps. Sassafras was more attractive to X. glabratus than previously reported and supported reproducing populations of the insect. End sealing bolts with a wax-and-water emulsion may not be optimal for attracting and rearing ambrosia beetles in small logs. C1 [Mayfield, A. E., III] US Forest Serv, USDA, So Res Stn, Asheville, NC 28804 USA. [Hanula, J. L.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. RP Mayfield, AE (reprint author), US Forest Serv, USDA, So Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM amayfield02@fs.fed.us FU USDA Forest Service Forest Health Protection and Southern Research Station FX We gratefully acknowledge the following people for technical or administrative assistance with this project: Jeff Eickwort, Ed Barnard, Dave Treadway, and Nicole Howard(Florida FDACS Division of Forestry); Jorge Pena, Alan Long, and the Austin Cary Memorial Forest (University of Florida); Greg Driskell (Plum Creek Timber Co.); Karolynne Griffiths (USDA-APHIS-CAPS); George Schneider and Wayne Dixon (Florida FDACS Division of Plant Industry); Bill Sweeney, William Elliott, Scott Horn, Mike Cody, Bob McCollum, Joe Davis, Chelcy Ford, Jim Vose (USDA Forest Service, Southern Research Station); Laurie Reid (South Carolina Forestry Commission); Rosemary Hallberg (North Carolina State University); Mark Danaher, Bill Twomey, Russ Marshall, and staff of the Witherbee Ranger Station (USDA Forest Service, Francis Marion National Forest). Funding for this work came from the USDA Forest Service Forest Health Protection and Southern Research Station. NR 34 TC 18 Z9 19 U1 1 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 461 EP 470 DI 10.1603/EC11348 PG 10 WC Entomology SC Entomology GA 925TL UT WOS:000302784300021 PM 22606816 ER PT J AU Epsky, ND Midgarden, D Rendon, P Villatoro, D Heath, RR AF Epsky, Nancy D. Midgarden, David Rendon, Pedro Villatoro, David Heath, Robert R. TI Efficacy of Wax Matrix Bait Stations for Mediterranean Fruit Flies (Diptera: Tephritidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Ceratitis capitata; spinosad; fruit fly management; ammonium acetate; trimethylamine ID CERATITIS-CAPITATA DIPTERA; APPLE MAGGOT FLIES; IN-FIELD TRIALS; SYNTHETIC ATTRACTANT; FLY; SPHERES; AMMONIA; FEMALES; CAPTURE; TRAPS AB Tests were conducted that evaluated efficacy of wax matrix bait stations for Ceratitis capitata (Wiedemann) adults in Guatemala. Bait stations were exposed to outdoor conditions to determine effect of weathering on longevity as indicated by bait station age. Results of laboratory tests found that bait stations with spinosad and ammonium acetate remained effective for at least 31 d compared with pesticide-free controls, although there was some loss of efficacy over time. Percentage mortality for bait station strips with 2% spinosad and 1% ammonium acetate decreased from 100 +/- 0.0% on day 0 to 70 +/- 7.1% after 31 d. Ammonia concentration had little effect on percentage mortality although there was some indication that ammonia concentration affected number of flies observed on the bait stations. Bait station strips (one per cage) were more effective than controls for 6-8 wk when tests were conducted in fleld cages (3 m diameter x 2 m), but only 2-3 wk when tests were conducted in large (2.5 m high and 6.0 m wide and 7.5 m long) field cages. Longevity was restored when multiple bait stations (3, 6, or 12) were deployed per cage. Bait stations containing methomyl were used for field tests of efficacy for wild flies. Dipped lure bait stations, which were made by coating two edges of commercial ammonium acetate and trimethylamine lures, killed six times more flies than corn cob bait stations dipped into a Nulure/malathion solution. They also killed more flies than pesticide-free controls for 8 wk. C1 [Epsky, Nancy D.; Midgarden, David; Heath, Robert R.] ARS, USDA, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Rendon, Pedro; Villatoro, David] APHIS, USDA, PPQ, Us Embassy, Guatemala. RP Epsky, ND (reprint author), ARS, USDA, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM nancy.epsky@ars.usda.gov NR 29 TC 1 Z9 3 U1 1 U2 7 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 471 EP 479 DI 10.1603/EC11147 PG 9 WC Entomology SC Entomology GA 925TL UT WOS:000302784300022 PM 22606817 ER PT J AU Hesler, LS Prischmann, DA Dashiell, KE AF Hesler, Louis S. Prischmann, Deirdre A. Dashiell, Kenton E. TI Field and Laboratory Evaluations of Soybean Lines Against Soybean Aphid (Hemiptera: Aphididae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Aphis glycines; Glycine max; host plant resistance; genotype; aphid management strategy ID SINGLE DOMINANT GENE; RESISTANCE; REGISTRATION; GERMPLASM; GLYCINES; GENOTYPES; JACKSON AB The soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), is a major pest of soybean, Glycine max (L.). Merr., that significantly reduces yield in northern production areas of North America. Insecticides are widely used to control soybean aphid outbreaks, but efforts are underway to develop host plant resistance as an effective alternative management strategy. Here, previously identified resistant lines were evaluated in laboratory tests against field-collected populations of soybean aphid and in field-plot tests over 2 yr in South Dakota. Six lines previously identified with resistance to soybean aphid-Jackson, Dowling, K1639, Cobb, Palmetto and Sennari-were resistant in this study, but relatively high aphid counts on Tie-feng 8 in field plots contrasted with its previously reported resistance. Bhart-PI 165989 showed resistance in one of two laboratory tests, but it had relatively large aphid infestations in both years of field tests. Intermediate levels of soybean aphid occurred in field plots on lines previously shown to have strong (Sugao Zairai, PI 230977, and D75-10169) or moderate resistance to soybean aphid (G93-9223, Bragg, Braxton, and Tracy-M). Sugao Zairai also failed to have a significant proportion of resistant plants in two laboratory tests against aphids field-collected in 2008, but it was resistant in laboratory tests with aphids collected in 2002, 2005, and 2006. Overall, results showed that lines with Rag (i.e., Jackson) or Rag1 gene (i.e., Dowling) had low aphid numbers, whereas lines with Rag2 (i.e., Sugao Zairai, Sennari) had mixed results. Collectively, responses of soybean aphid populations in laboratory and field tests in 2008 resembled a virulence pattern reported previously for biotype 3 soybean aphids, but virulence in soybean aphid populations was variable and dynamic over years of the study. These results, coupled with previous reports of biotypes virulent to Rag1, suggest that deployment of lines with a single aphid-resistance gene is limited for soybean aphid management, and that deployment strategies relying on multiple resistance genes may be needed to effectively use plant resistance against soybean aphid. C1 [Hesler, Louis S.; Prischmann, Deirdre A.; Dashiell, Kenton E.] USDA ARS, N Cent Agr Res Lab, Brookings, SD 57006 USA. RP Hesler, LS (reprint author), USDA ARS, N Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM louis.hesler@ars.usda.gov NR 41 TC 7 Z9 7 U1 2 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 608 EP 615 DI 10.1603/EC11339 PG 8 WC Entomology SC Entomology GA 925TL UT WOS:000302784300038 PM 22606833 ER PT J AU Diaz-Montano, J Fail, J Deutschlander, M Nault, BA Shelton, AM AF Diaz-Montano, John Fail, Jozsef Deutschlander, Mark Nault, Brian A. Shelton, Anthony M. TI Characterization of Resistance, Evaluation of the Attractiveness of Plant Odors, and Effect of Leaf Color on Different Onion Cultivars to Onion Thrips (Thysanoptera: Thripidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Thrips tabaci; Allium cepa; onion resistance; olfactometer; leaf color ID YELLOW-SPOT-VIRUS; WESTERN FLOWER THRIPS; TABACI LINDEMAN THYSANOPTERA; NEW-YORK; FRANKLINIELLA-OCCIDENTALIS; INSECTICIDE RESISTANCE; ECONOMIC THRESHOLDS; GROWTH-STAGES; B RADIATION; FIELDS AB Onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae), is a worldwide pest of onion, Allium cepa L. In field studies on onion resistance conducted in 2007 and 2008 using 49 cultivars, 11 showed low leaf damage by T. tabaci. In laboratory studies, the 11 cultivars, along with two susceptible checks and four additional cultivars, were evaluated to characterize resistance to T. tabaci and to determine if color and/or light reflectance were associated with resistance to T. tabaci. No-choice tests were performed with adults and the numbers of eggs and larvae were counted on each cultivar after three and 10 d, respectively. In choice tests in which all cultivars were planted together in a circle in a single pot, 100 adults were released and the number of adults on each plant was evaluated 24 h later. The behavioral response of walking T. tabaci adults to plant odors was studied in a glass Y-tube olfactometer. The reflectance spectrum of leaves was measured using a UV-VIS spectrophotometer. Results indicate that resistant cultivars showed an intermediate-high antibiotic effect to T. tabaci and all of them showed a very strong antixenotic effect. There were no significant preferences in the response of walking T. tabaci adults to plant odors. The two susceptible cultivars had the highest values of leaf reflectance for the first (275-375 nm) and second (310-410 nm) theoretical photopigment-system of T. tabaci, and these values were significantly different from most resistant cultivars. These results suggest a strong response of T. tabaci to onion cultivars with higher reflectance in the ultraviolet range (270-400 nm). Overall, these results appear promising in helping to identify categories of resistance to T. tabaci in onions that can be used in breeding programs. C1 [Diaz-Montano, John; Nault, Brian A.; Shelton, Anthony M.] Cornell Univ NYSAES, Dept Entomol, Geneva, NY 14456 USA. [Fail, Jozsef] Corvinus Univ Budapest, Dept Entomol, H-1118 Budapest, Hungary. [Deutschlander, Mark] Hobart & William Smith Coll, Dept Biol, Geneva, NY 14456 USA. RP Diaz-Montano, J (reprint author), USDA ARS Ctr Grain & Anim Hlth Res, Stored Product Insect Res Unit, 1515 Coll Av, Manhattan, KS 66502 USA. EM jd325@cornell.edu RI Fail, Jozsef/A-5429-2011 FU New York State Onion Research and Development Program; New York Farm Viability Initiative; Griswold Endowment, Department of Entomology, Cornell University FX We thank Christy Hoepting and Carol MacNeil (Cornell), Jan Van Der Heide (Bejo Seeds), Howard Schwartz and Michael Bartolo (CSU), and Chris Cramer (NMSU) for providing seeds; Marc Fuchs, Phillip Griffiths, Akiko Seto, Francoise Vermeylen, John Barnard, Mao Chen, Mei Cheung, Hilda Collins, Anuar Morales, Xiaoxia Liu, Yunhe Li, Cynthia (Simon) Hsu, Derek Battin, and Alvaro Romero for helping in different aspects of this study. This research was partially funded by the New York State Onion Research and Development Program and the New York Farm Viability Initiative. The publication costs of this research paper were funded by the Griswold Endowment, Department of Entomology, Cornell University. NR 67 TC 8 Z9 11 U1 0 U2 40 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 632 EP 641 DI 10.1603/EC11233 PG 10 WC Entomology SC Entomology GA 925TL UT WOS:000302784300041 PM 22606836 ER PT J AU Baluch, SD Ohm, HW Shukle, JT Williams, CE AF Baluch, Stephen D. Ohm, Herbert W. Shukle, John T. Williams, Christie E. TI Obviation of Wheat Resistance to the Hessian Fly Through Systemic Induced Susceptibility SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE systemic induced susceptibility; Hessian fly; wheat; resistance; susceptibility ID PLANT DEFENSE; DIPTERA CECIDOMYIIDAE; UNITED-STATES; LARVAE; GENES; VIRULENCE; SOUTHEASTERN; OVIPOSITION; PENETRATION; EXPRESSION AB Unlike most documented plant-insect interactions, Hessian fly-resistance [Mayetiola destructor (Say)] in wheat (Triticum aestivum L.) is initiated by a gene-for-gene recognition event in which plants carrying a specific R gene recognize salivary effectors encoded by a corresponding larval avirulence gene. However, dual infestation resulting from oviposition by virulent insects from 5 d before to 3 d after oviposition by avirulent insects on the same host plant, lead to systemic induced susceptibility, obviation of resistance, and ultimately the survival of both virulent and genetically avirulent progeny to adulthood. Simultaneous oviposition allowed greater survival of avirulent progeny than ovipositions separated by larger intervals. Because of the induction of plant resistance, hatch of avirulent larvae before virulent was more detrimental to rate of development than hatch of virulent before avirulent larvae. Obviation of resistance was not localized to the leaf being attacked by the virulent larvae, but also functioned across spatial distance into younger leaves. This research suggests that virulent Hessian fly larvae directly suppress the defense response of wheat, thus providing a refuge for avirulent genotypes, preserving diversity in field populations and increasing durability of deployed resistance genes. C1 [Shukle, John T.; Williams, Christie E.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Williams, Christie E.] USDA ARS MWA, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Baluch, Stephen D.; Ohm, Herbert W.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Williams, CE (reprint author), Purdue Univ, Dept Entomol, 901 W State St, W Lafayette, IN 47907 USA. EM christie.williams@ars.usda.gov FU USDA CRIS [3602-22000-016-D] FX We thank Jill Nemacheck and Andrea Hargarten for assistance in preparing the manuscript and Sue Cambron for maintaining Hessian fly stocks. This is a joint contribution by the United States Department of Agriculture-Agricultural Research Service and Purdue University. Research was supported by USDA CRIS number 3602-22000-016-D. NR 29 TC 7 Z9 7 U1 2 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 642 EP 650 DI 10.1603/EC11329 PG 9 WC Entomology SC Entomology GA 925TL UT WOS:000302784300042 PM 22606837 ER PT J AU Jackson, DM Harrison, HF Ryan-Bohac, JR AF Jackson, D. Michael Harrison, Howard F., Jr. Ryan-Bohac, J. R. TI Insect Resistance in Sweetpotato Plant Introduction Accessions SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE plant resistance; Ipomoea; Diabrotica; Cylas formicarius; white grub ID MULTIPLE-PEST; WEEVIL; CULTIVARS; COLEOPTERA; GENOTYPES; REGAL AB Fifty-five sweetpotato cultivars, experimental breeding clones, and plant introduction (PI) accessions were evaluated in 17 field experiments at the USDA, ARS, U. S. Vegetable Laboratory (Charleston, SC; 12 evaluations, 1997-2010), the Clemson University, Edisto Research and Education Center (Blackville, SC; two evaluations, 1998-1999), and the University of Florida, Tropical Research and Education Center (Homestead, FL; three evaluations, 2005-2007). These experiments included two insect-susceptible control entries ('Beauregard' and 'SC1149-19') and three insect-resistant control cultivars ('Regal,' 'Ruddy,' and 'Sumor'). At each location, genotypes differed significantly in the percentage of uninjured roots WDS (wireworm, Diabrotica, Systena) index, the percentage of roots damaged by the sweetpotato weevil (Cylas formicarius (F.)), the percentage of roots damaged by the sweetpotato flea beetle (Chaetocnema confinis Crotch), and the percentage of roots damaged by white grub larvae (including Plectris aliena Chapin and Phyllophaga spp.). 'SC1149-19' had a significantly lower percentage of uninjured roots, a significantly higher WDS index rating, and significantly higher percentages of infestation by flea beetles, grubs, and sweetpotato weevils than most other sweetpotato genotypes in this study. In addition, 43 of 55 genotypes had significantly less overall insect damage than 'Beauregard,' one of the leading commercial orange-fleshed cultivars in the United States. Ten genotypes had significantly less insect injury than 'Picadito,' a commercial boniato-type sweetpotato grown extensively in southern Florida. Many of these sweetpotato genotypes have high levels of resistance to soil insect pests, and they may be useful as sources of insect resistance for use in sweetpotato breeding programs. C1 [Jackson, D. Michael; Harrison, Howard F., Jr.; Ryan-Bohac, J. R.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Jackson, DM (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM mike.jackson@.ars.usda.gov NR 59 TC 2 Z9 3 U1 0 U2 8 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 651 EP 658 DI 10.1603/EC11307 PG 8 WC Entomology SC Entomology GA 925TL UT WOS:000302784300043 PM 22606838 ER PT J AU Kendra, PE Niogret, J Montgomery, WS Sanchez, JS Deyrup, MA Pruett, GE Ploetz, RC Epsky, ND Heath, RR AF Kendra, Paul E. Niogret, Jerome Montgomery, Wayne S. Sanchez, Jorge S. Deyrup, Mark A. Pruett, Grechen E. Ploetz, Randy C. Epsky, Nancy D. Heath, Robert R. TI Temporal Analysis of Sesquiterpene Emissions From Manuka and Phoebe Oil Lures and Efficacy for Attraction of Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Redbay ambrosia beetle; laurel wilt; avocado; alpha-copaene; sesquiterpene ID REDBAY AMBROSIA BEETLE; SOUTHEASTERN UNITED-STATES; LAUREL WILT; RAFFAELEA-LAURICOLA; AVOCADO; LAURACEAE; PATHOGEN; TREES; DIVERSITY; SYMBIONT AB Redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is an exotic wood-borer that vectors the fungal agent (Raffaelea lauricola) responsible for laurel wilt. Laurel wilt has had severe impact on forest ecosystems in the southeastern United States, killing a large proportion of native Persea trees, particularly redbay (P. borbonia) and swampbay (P. palustris), and currently poses an economic threat to avocado (P. americana) in Florida. To control the spread of this lethal disease, effective attractants are needed for early detection of the vector. Two 12-wk field tests were conducted in Florida to evaluate efficacy and longevity of manuka and phoebe oil lures, and to relate captures of X. glabratus to release rates of putative sesquiterpene attractants. Two trap types were also evaluated, Lindgren funnel traps and sticky panel traps. To document lure emissions over time, a separate set of lures was aged outdoors for 12 wk and sampled periodically to quantify volatile sesquiterpenes using super-Q adsorbant and gas chromatography-mass spectroscopy analysis. Phoebe lures captured significantly more X. glabratus than manuka lures, and sticky traps captured more beetles than funnel traps. Phoebe lures captured X. glabratus for 10-12 wk, but field life of manuka lures was 2-3 wk. Emissions of alpha-copaene, alpha-humulene, and cadinene were consistently higher from phoebe lures, particularly during the 2-3 wk window when manuka lures lost efficacy, suggesting that these sesquiterpenes are primary kairomones used by host-seeking females. Results indicate that the current monitoring system is suboptimal for early detection of X. glabratus because of rapid depletion of sesquiterpenes from manuka lures. C1 [Kendra, Paul E.; Niogret, Jerome; Montgomery, Wayne S.; Sanchez, Jorge S.; Epsky, Nancy D.; Heath, Robert R.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Deyrup, Mark A.; Pruett, Grechen E.] Archbold Biol Stn, Lake Placid, FL 33862 USA. [Ploetz, Randy C.] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. RP Kendra, PE (reprint author), USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM paul.kendra@ars.usda.gov FU USDA-ARS National Plant Disease Recovery System; NIFA; Florida Avocado Administrative Committee FX We gratefully acknowledge David Long, Ricardo Joseph (USDA-ARS; Miami, FL), and Ken Dearborn (Archbold Biological Station, Lake Placid, FL) for technical assistance; Kate Okins and Patti Anderson (FDACS-DPI; Gainesville, FL) for identifications of Scolytinae and Persea, respectively; Amy Roda (APHIS-PPQ, Miami, FL), David Jenkins (USDA-ARS, Mayaguez, PR), and three journal reviewers for helpful suggestions with the manuscript; and Connie Rightmire (St. John's River Water Management District; Palatka, FL) and Shane Belson (Florida Fish and Wildlife Conservation Commission; St. Cloud, FL) for assistance in obtaining special use permits for the Lochloosa Wildlife Conservation Area and the Lake Wales Ridge Wildlife Management Area, respectively. This work was supported in part by the USDA-ARS National Plant Disease Recovery System, a NIFA Critical Issues Grant, and the Florida Avocado Administrative Committee. NR 45 TC 18 Z9 18 U1 0 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 659 EP 669 DI 10.1603/EC11398 PG 11 WC Entomology SC Entomology GA 925TL UT WOS:000302784300044 PM 22606839 ER PT J AU Semeao, AA Campbell, JF Whitworth, RJ Sloderbeck, PE AF Semeao, Altair A. Campbell, James F. Whitworth, Robert J. Sloderbeck, Phillip E. TI Influence of Environmental and Physical Factors on Capture of Tribolium castaneum (Coleoptera: Tenebrionidae) in a Flour Mill SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Tribolium castaneum; spatial distribution; flour mill; fumigation; integrated pest management ID STORED-PRODUCT INSECTS; MOTH LEPIDOPTERA; BAITED TRAPS; TEMPERATURE; FUMIGATION; PYRALIDAE; MOVEMENT; DYNAMICS; CONFUSUM; BEETLES AB Variation in environmental and physical factors within food processing facilities can influence both the distribution of stored-product pests and trapping efficiency. Data from a long-term Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) monitoring program was used to evaluate spatial variation in captures among trap locations and to determine relationships with environmental and physical variables. From the complete monitoring data set, different subsets were created for the cool and warm seasons, and period of time when environmental and physical factors were measured (2009-2010), with all data sets showing significant differences among trap locations in terms of beetle captures and proportion of time that traps exceeded 2.5 beetles per trap per monitoring period. There was also considerable temporal variation in distribution among the different levels of the mill. Among the environmental and physical variables measured, mean temperature and flour dust accumulation showed the most significant positive relationships with variation of beetle captures at trap locations. More beetles were captured in traps located in close proximity to milling equipment. Presence of equipment near traps was also associated with an increase in flour dust accumulation and temperature. Overall the environmental and physical factors seemed to have a limited influence on variation in captures among trap locations, with temporal variation in distribution perhaps overwhelming potential influences of local trap conditions. C1 [Campbell, James F.] USDA ARS, CGAHR, Manhattan, KS 66502 USA. [Sloderbeck, Phillip E.] Kansas State Univ, SW Res & Extens Ctr, Garden City, KS 67846 USA. [Campbell, James F.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Semeao, Altair A.; Whitworth, Robert J.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Campbell, JF (reprint author), USDA ARS, CGAHR, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov FU USDA-CSREES-RAMP [2005-51101-02358]; USDA Methyl Bromide Alternative [2006-51102-03588] FX We thank the flour mill personnel for allowing access to their facility and R. Hammel, M. Hartzer, and C. Belda for assistance with the data collection. This study was supported in part by USDA-CSREES-RAMP grant 2005-51101-02358 and USDA Methyl Bromide Alternative grant 2006-51102-03588. This article is contribution 12-083-J from the Kansas Agricultural Experiment Station. NR 39 TC 9 Z9 9 U1 1 U2 9 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 686 EP 702 DI 10.1603/EC11322 PG 17 WC Entomology SC Entomology GA 925TL UT WOS:000302784300047 PM 22606842 ER PT J AU Brijwani, M Subramanyam, B Flinn, PW AF Brijwani, Monika Subramanyam, Bhadriraju Flinn, Paul W. TI Impact of Varying Levels of Sanitation on Mortality of Tribolium castaneum Eggs and Adults During Heat Treatment of a Pilot Flour Mill SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE heat treatment; temperature; sanitation; red flour beetle; efficacy assessment ID LIFE STAGES; TENEBRIONIDAE; COLEOPTERA; FEED AB The influence of sanitation on responses of life stages of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), an economically important pest in flour mills, was investigated in a pilot flour mill subjected to two, 24-h heat treatments. One hundred eggs or 100 adults of T. castaneum were exposed inside each 20-cm diameter by 15-cm high PVC rings holding 0.1-, 0.2-, 1.0-, 3.0-, 6.0-, and 10.0-cm-deep wheat flour to simulate different sanitation levels that may exist in a flour mill. These rings were placed on the first and third floors of a pilot flour mill. On the first floor, temperatures inside rings with eggs reached 50 degrees C in 7-11 h only in 0.1- and 0.2- cm-deep flour treatments. In all other treatments the maximum temperatures attained generally were below 50 degrees C and inversely related to flour depth. Adults of T. castaneum on this floor were less susceptible than eggs. The egg mortality decreased linearly with an increase in flour depth, whereas that of adults decreased exponentially. All eggs and adults in rings on the third floor were killed irrespective of flour depth, because temperatures inside rings reached 50 degrees C in 15-17 h and were held above 50 degrees C for 6-8 h with the maximum temperatures ranging between 55.0 and 57.0 degrees C. Although the protective effects of flour on survival of T. castaneum eggs and adults were evident only if temperatures did not reach 50 degrees C, removal of flour accumulations is essential to improve heat treatment effectiveness. C1 [Brijwani, Monika; Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Flinn, Paul W.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Subramanyam, B (reprint author), Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. EM sbhadrir@ksu.edu RI Flinn, Paul/B-9104-2013 FU USDA/CSREES (NIFA) [2008-51102-04583]; Propane Education and Research Council (PERC), WA, DC FX Research reported here was supported by grants from USDA/CSREES (NIFA) Methyl Bromide Transitions Program grant under agreement number 2008-51102-04583, and from The Propane Education and Research Council (PERC), WA, DC. This paper is contribution number 11-311-J of the Kansas State University Agricultural Experiment Station. NR 22 TC 3 Z9 3 U1 0 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 703 EP 708 DI 10.1603/EC11115 PG 6 WC Entomology SC Entomology GA 925TL UT WOS:000302784300048 PM 22606843 ER PT J AU Brijwani, M Subramanyam, B Flinn, PW Langemeier, MR Hartzer, M Hulasare, R AF Brijwani, Monika Subramanyam, Bhadriraju Flinn, Paul W. Langemeier, Michael R. Hartzer, Michelle Hulasare, Rajshekhar TI Susceptibility of Tribolium castaneum Life Stages Exposed to Elevated Temperatures during Heat Treatments of a Pilot Flour Mill: Influence of Sanitation, Temperatures Attained Among Mills Floors, and Costs SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE heat treatment; temperature; sanitation; insect mortality; economics ID COLEOPTERA; TENEBRIONIDAE; INSECTS AB The influence of sanitation on responses of life stages of the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), was investigated in a pilot flour mill subjected to three, 24-h heat treatments by using forced-air gas heaters fueled by propane. Two sanitation levels, dusting of wheat flour and 2-cm-deep flour, were created in 25 plastic bioassay boxes, each holding eggs, young larvae, old larvae, pupae, and adults of T. castaneum plus two temperature sensors. Data loggers (48) were placed on the five mill floors to record air temperatures. The time required to reach 50 degrees C, time above 50 degrees C, and the maximum temperature among mill floors and in bioassay boxes were measured. The maximum temperature in bioassay boxes and in the mill was lower on the first floor than on other floors. This trend was apparent in time required to reach 50 degrees C and time above 50 degrees C, especially in compartments with 2-cm-deep flour. The mean +/- SE mortality of T. castaneum life stages on the first floor was 55.5 +/- 12.9-98.6 +/- 0.8%; it was 93.2 +/- 6.7-100 +/- 0.0% on other floors. Adults were the least susceptible stage. Mortality of T. castaneum stages in compartments with 2-cm-deep flour was generally lower than those with flour dust. Costs for the three heat treatments ranged from US$27,438 to $28,838. An effective heat treatment can be conducted within 24 h, provided temperatures on mill floors reach 50 degrees C in 8-12 h and are held above 50 degrees C for at least 10-14 h, with maximum temperatures held between 50 and 60 degrees C. C1 [Brijwani, Monika; Subramanyam, Bhadriraju; Hartzer, Michelle] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Flinn, Paul W.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Langemeier, Michael R.] Kansas State Univ, Dept Agr Econ, Manhattan, KS 66506 USA. [Hulasare, Rajshekhar] Temp Air Inc, Burnsville, MN 55337 USA. RP Subramanyam, B (reprint author), Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. EM sbhadrir@ksu.edu RI Flinn, Paul/B-9104-2013 FU USDA-CSREES (NIFA) [2008-51102-04583] FX We thank Jim Weaver, Alex Young, Xue Meng, Sara Savoldelli, and Khamis Moses for help with the insect bioassays. We are grateful to Mark West for statistical help and to David Hagstrum for reviewing an earlier draft of this manuscript. Research reported here was supported by a grant from USDA-CSREES (NIFA) Methyl Bromide Transitions Program under agreement 2008-51102-04583. The Propane Education and Research Council (PERC), Washington, DC, underwrote all heat treatment expenses, including a hands-on heat treatment workshop (http://www.ksre.ksu.edu/grsc_subi/Conference/confrence.htm). This paper is contribution 11-310-J of the Kansas State University Agricultural Experiment Station. NR 19 TC 4 Z9 4 U1 0 U2 12 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 709 EP 717 DI 10.1603/EC11114 PG 9 WC Entomology SC Entomology GA 925TL UT WOS:000302784300049 PM 22606844 ER PT J AU Wijayaratne, LKW Fields, PG Arthur, FH AF Wijayaratne, L. K. Wolly Fields, Paul G. Arthur, Frank H. TI Residual Efficacy of Methoprene for Control of Tribolium castaneum (Coleoptera: Tenebrionidae) Larvae at Different Temperatures on Varnished Wood, Concrete, and Wheat SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE methoprene; concrete; varnished wood; residual efficacy; surface treatment ID CYFLUTHRIN WETTABLE POWDER; STORED-PRODUCT BEETLES; CONFUSUM COLEOPTERA; CHLORPYRIFOS-METHYL; S-METHOPRENE; FLOUR BEETLE; IMPACT; FOOD; INSECTICIDES; RESISTANCE AB The residual efficacy of the juvenile hormone analog methoprene (Diacon II) was evaluated in bioassays using larvae of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) exposed on unsealed concrete or varnished wood treated with a liquid formulation and held at different temperatures. When these two types of surfaces were stored at 20, 30 or 35 degrees C for 0-24 wk, the percentage of adult emergence on concrete increased with time. In contrast, there was no adult emergence from larvae exposed to varnished wood at 24 wk after treatment at any of these temperatures. The presence of flour reduced residual efficacy of methoprene on concrete, but not on varnished wood, with no differences between cleaning frequencies. Methoprene was also stable for 48 h on concrete held at 65 degrees C and wheat, Triticum aestivum L., held at 46 degrees C. Results show that methoprene is stable at a range of temperatures commonly encountered in indoor food storage facilities and at high temperatures attained during insecticidal heat treatments of structures. The residual persistence of methoprene applied to different surface substrates may be affected more by the substrate than by temperature. C1 [Wijayaratne, L. K. Wolly; Fields, Paul G.] Agr & Agri Food Canada, Cereal Res Ctr, Winnipeg, MB R3T 2M9, Canada. [Wijayaratne, L. K. Wolly] Univ Manitoba, Dept Entomol, Winnipeg, MB R3T 2N2, Canada. [Arthur, Frank H.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Fields, PG (reprint author), Agr & Agri Food Canada, Cereal Res Ctr, 195 Dafoe Rd, Winnipeg, MB R3T 2M9, Canada. EM paul.felds@agr.gc.ca FU University of Manitoba; Manitoba Graduate Scholarship FX We thank Neil Holliday, Steve Whyard (University of Manitoba) and Desiree Vanderwel (University of Winnipeg) for contributions on this research. The chemicals for the study were provided by Central Life Sciences. We thank Thomas Phillips (Kansas State University) and James Throne (USDA-ARS-CGAHR) for reviewing this manuscript before submission. Financial support to the first author through University of Manitoba Graduate Fellowship and Manitoba Graduate Scholarship is appreciated. NR 45 TC 14 Z9 17 U1 0 U2 9 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 718 EP 725 DI 10.1603/EC11375 PG 8 WC Entomology SC Entomology GA 925TL UT WOS:000302784300050 PM 22606845 ER PT J AU Taylor, DB Friesen, K Zhu, JJ Sievert, K AF Taylor, D. B. Friesen, K. Zhu, J. J. Sievert, K. TI Efficacy of Cyromazine to Control Immature Stable Flies (Diptera: Muscidae) Developing in Winter Hay Feeding Sites SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Stomoxys calcitrans; insect growth regulator; larval development ID MUSCA-DOMESTICA L.; HOUSE-FLY DIPTERA; INSECTICIDE RESISTANCE; TREATED TARGETS; CATTLE; MANURE; LARVADEX; POULTRY; POPULATIONS; BALES AB Hay mixed with manure and urine residues at sites where hay has been provided as supplemental winter feed for cattle provide an excellent substrate for the development of immature stable flies, Stomoxys calcitrans (L.). Such sites are primary sources of early summer stable flies in the central United States and no effective measures are currently available to control fly development in them. A single application of granular cyromazine in May provided 97% reduction in the number of adult stable flies emerging from hay feeding sites. Stable fly control did not decline during the 12 wk season. A small decline in control was observed relative to anthomyiid, sarcophagid, and syrphid flies developing in the hay feeding sites. However, none of those flies are considered to be pests and >= 50% control of those flies was maintained for 65 d after application. Cyromazine offers a safe and affordable option for the control of immature stable flies developing in winter hay feeding sites. Controlling those flies should reduce the estimated $2 billion per year of lost production in U.S. cattle industries attributable to stable flies. C1 [Taylor, D. B.; Friesen, K.; Zhu, J. J.] Univ Nebraska, USDA ARS Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Taylor, D. B.; Friesen, K.; Zhu, J. J.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Sievert, K.] Novartis Anim Hlth Inc, CH-4058 Basel, Switzerland. RP Taylor, DB (reprint author), Univ Nebraska, USDA ARS Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. EM dave.taylor@ars.usda.gov RI Taylor, David/G-6025-2014; OI Taylor, David/0000-0002-4378-4867; Zhu, Junwei/0000-0001-9846-9656 FU Novartis Animal Health, Inc.; Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln [S-1030] FX We thank Karl Moline and the University of Nebraska Agricultural Research and Development Center for access to facilities and logistical support. Dennis Berkebile, Doug Brinkerhoff, Briana Gaston, Andrew German, Megan Himmelberg, Eli Olsen, Brad Voelker, and Brandon White provided technical assistance. This study was partially funded by Novartis Animal Health, Inc. and was done in cooperation with the Institute of Agriculture and Natural Resources, University of Nebraska, Lincoln as a contributor to multistate project S-1030. NR 27 TC 6 Z9 7 U1 0 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2012 VL 105 IS 2 BP 726 EP 731 DI 10.1603/EC11317 PG 6 WC Entomology SC Entomology GA 925TL UT WOS:000302784300051 PM 22606846 ER PT J AU Huang, LH Tu, SI Phillips, J Fratamico, P AF Huang, Lihan Tu, Shu-I Phillips, John Fratamico, Pina TI Mathematical Modeling of Growth of Non-O157 Shiga Toxin-Producing Escherichia coli in Raw Ground Beef SO JOURNAL OF FOOD SCIENCE LA English DT Article DE ground beef; kinetic analysis; Shiga toxin-producing Escherichia coli; predictive microbiology ID HEMOLYTIC-UREMIC-SYNDROME; LISTERIA-MONOCYTOGENES; BACTERIAL-GROWTH; INFECTIONS; OUTBREAK; KINETICS; FOOD AB The objective of this study was to investigate the growth of Shiga toxin-producing Escherichia coli (STEC, including serogroups O45, O103, O111, O121, and O145) in raw ground beef and to develop mathematical models to describe the bacterial growth under different temperature conditions. Three primary growth models were evaluated, including the Baranyi model, the Huang 2008 model, and a new growth model that is based on the communication of messenger signals during bacterial growth. A 5 strain cocktail of freshly prepared STEC was inoculated to raw ground beef samples and incubated at temperatures ranging from 10 to 35 degrees C at 5 degrees C increments. Minimum relative growth (<1 log10 cfu/g) was observed at 10 degrees C, whereas at other temperatures, all 3 phases of growth were observed. Analytical results showed that all 3 models were equally suitable for describing the bacterial growth under constant temperatures. The maximum cell density of STEC in raw ground beef increased exponentially with temperature, but reached a maximum of 8.53 log10 cfu/g of ground beef. The specific growth rates estimated by the 3 primary models were practically identical and can be evaluated by either the Ratkowsky square-root model or a Belehradek-type model. The temperature dependence of lag phase development for all 3 primary models was also developed. The results of this study can be used to estimate the growth of STEC in raw ground beef at temperatures between 10 and 35 degrees C. C1 [Huang, Lihan; Tu, Shu-I; Phillips, John; Fratamico, Pina] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 22 TC 1 Z9 1 U1 1 U2 6 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD APR PY 2012 VL 77 IS 4 BP M217 EP M225 DI 10.1111/j.1750-3841.2012.02647.x PG 9 WC Food Science & Technology SC Food Science & Technology GA 928QT UT WOS:000302998600040 PM 22515248 ER PT J AU Sarnoski, PJ Boyer, RR O'Keefe, SF AF Sarnoski, Paul J. Boyer, Renee R. O'Keefe, Sean F. TI Application of Proanthocyanidins from Peanut Skins as a Natural Yeast Inhibitory Agent SO JOURNAL OF FOOD SCIENCE LA English DT Article DE natural antimicrobial; optical density; peanut skin; proanthocyanidin; yeast ID SACCHAROMYCES-CEREVISIAE; LISTERIA-MONOCYTOGENES; PATHOGENIC YEASTS; SPOILAGE YEASTS; PLANT-EXTRACTS; ESSENTIAL OILS; MEMBRANE; COUNT; SUSCEPTIBILITY; ANTIMICROBIALS AB Proanthocyanidins were extracted from peanut skins and investigated for their antimicrobial activity against Saccharomyces cerevisiae, Zygosaccharomyces bailii, and Zygosaccharomyces bisporus in traditional growth media (Sabouraud Dextrose and Maltose broth) and a simulated apple juice beverage. Peanut skins extracts (PSE) were prepared through a multisolvent extraction procedure. The PSE extended the lag phase growth of the 3 yeasts studied at a concentration of 1 mg/mL and at 10 mg/mL yeast growth was totally inhibited for 120 h. PSE was fractionated by normal phase high performance liquid chromatography and the active components/fractions were determined. Compounds present in the fractions were identified by liquid chromatography-mass spectrometry to determine the compounds responsible for inhibition. Fractions consisting mostly of A-type proanthocyanidin dimers, trimers, and tetramers showed the highest percent inhibition toward the yeasts tested in this study. Both optical density (OD) and standard enumeration plating methods were performed in this study. The OD method led to an overestimation of the inhibitory effects of PSE, the 2 methods agreed in respect to treatment effects but not the severity of the inhibition. C1 [Sarnoski, Paul J.] ARS, USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Boyer, Renee R.; O'Keefe, Sean F.] Virginia Polytech Inst & State Univ, Dept Food Sci & Technol, Blacksburg, VA 24061 USA. RP Sarnoski, PJ (reprint author), ARS, USDA, Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM paul.sarnoski@ars.usda.gov RI O'Keefe, Sean/N-3101-2016 OI O'Keefe, Sean/0000-0002-2491-7900 FU Biodesign and Bioprocessing Research Center (BBRC) of Virginia Tech FX This work was supported by the Biodesign and Bioprocessing Research Center (BBRC) of Virginia Tech. NR 39 TC 4 Z9 5 U1 1 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD APR PY 2012 VL 77 IS 4 BP M242 EP M249 DI 10.1111/j.1750-3841.2012.02652.x PG 8 WC Food Science & Technology SC Food Science & Technology GA 928QT UT WOS:000302998600044 PM 22515250 ER PT J AU Brandon, DL Korn, AM Yang, LL AF Brandon, David L. Korn, Anna M. Yang, Lily L. TI Detection of Ricin Contamination in Liquid Egg by Electrochemiluminescence Immunosorbent Assay SO JOURNAL OF FOOD SCIENCE LA English DT Article DE biothreat; castor; electrochemiluminescence; monoclonal antibody; ricin ID POLYMERASE-CHAIN-REACTION; BIOLOGICAL-ACTIVITY; FOOD; VALIDATION; ELISA; MILK AB A monoclonal antibody-based electrochemical luminescence method was developed for detecting and quantifying ricin in liquid egg, with a limit of detection of 0.2 ng/mL. Because this highly toxic protein, present in the seeds of Ricinus communis (castor), has been used for intentional poisoning in the past, it is important to have sensitive and reliable analytical methodology to detect ricin in food matrices such as liquid egg. The detection of this quantity of pure or crude ricin spiked into commercial samples of liquid egg provides approximately 50000-fold greater sensitivity than required to detect a toxic dose of ricin (>1 mg) in a 100 g sample. C1 [Brandon, David L.; Korn, Anna M.; Yang, Lily L.] ARS, Foodborne Contaminants Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Brandon, DL (reprint author), ARS, Foodborne Contaminants Res Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM david.brandon@ars.usda.gov NR 25 TC 7 Z9 7 U1 2 U2 12 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD APR PY 2012 VL 77 IS 4 BP T83 EP T88 DI 10.1111/j.1750-3841.2012.02627.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 928QT UT WOS:000302998600057 PM 22429217 ER PT J AU Sterling, KM Okech, BA Xiang, MHA Linser, PJ Price, DA VanEkeris, L Becnel, JJ Harvey, WR AF Sterling, Kenneth M. Okech, Bernard A. Xiang, Minghui A. Linser, Paul J. Price, David A. VanEkeris, Leslie Becnel, James J. Harvey, William R. TI High affinity H-3-phenylalanine uptake by brush border membrane vesicles from whole larvae of Aedes aegypti (AaBBMVw) SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE AaNAT8; AgNAT8; H+ V-ATPase; AgNHA1; VAN ID AMINO-ACID-TRANSPORT; LEUCINE TRANSPORT; ANOPHELES-GAMBIAE; K+/H+ ANTIPORT; MIDGUT; PHENYLALANINE; SYMPORTER; EXCHANGER; PATHWAY; CLONING AB Brush border membrane vesicles from whole Aedes aegypti larvae (AaBBMVw) are confirmed to be valid preparations for membrane transport studies. The Abdul-Rauf and Ellar method was used to isolate AaBBMVw that were frozen, stored for several months, transported to a distant site, thawed and used to study Na+-coupled, H-3-labeled, phenylalanine (Phe) uptake. The affinity for all components of the uptake was very high with half maximal values in the sub-micromolar range. By contrast a K-0.5(Phe) of 0.2 mM and a K-0.5(Na) mM were calculated from Phe-induced electrical currents in Xenopus oocytes that were heterologously expressing the Anopheles gambiae symporter (co-transporter). AgNAT8, in a buffer with 98 mM Na+. What accounts for the >1000-fold discrepancy in affinity for substrates between the BBMV and oocyte experiments? Is it because Ae. aegypti were used to isolate BBMVw whereas An. gambiae were used to transfect oocytes? More likely, it is because BBMVw were exposed to [Na+] in the micromolar range with the transporter(s) being surrounded by native lipids. By contrast, the oocyte measurements were made at [Na+] 100,000 times higher with AgNAT8 surrounded by foreign frog lipids. The results show that AaBBMVw are osmotically sealed: the time-course has a Na+-induced overshoot, the pH optimum is similar to 7 and the K-0.5 values for Phe and Na+ are very low. The transport is virtually unchanged when Na+ is replaced by K+ or Li+ but decreased by Rb+. This approach to resolving discrepancies between electrical data on solute transporters such as AgNAT8 that are over-expressed in oocytes and flux data on corresponding transporters that are highly expressed in native membrane vesicles, may serve as a model for similar studies that add membrane biochemistry to molecular biology in efforts to identify targets for the development of new methods to control disease-vector mosquitoes. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Sterling, Kenneth M.; Linser, Paul J.; Price, David A.; VanEkeris, Leslie; Harvey, William R.] Univ Florida, Whitney Mosquito Biol Grp, St Augustine, FL 32080 USA. [Okech, Bernard A.; Harvey, William R.] Univ Florida, Dept Environm & Global Hlth, Coll Publ Hlth & Hlth Profess, Gainesville, FL 32610 USA. [Okech, Bernard A.; Harvey, William R.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA. [Xiang, Minghui A.] Univ Florida, Div Nephrol & Hypertens, Jacksonville, FL 32206 USA. [Becnel, James J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Xiang, Minghui A.; Harvey, William R.] Univ Florida, Coll Med, Dept Physiol & Funct Genom, Gainesville, FL 32610 USA. RP Harvey, WR (reprint author), Univ Florida, Whitney Mosquito Biol Grp, 9505 Ocean Shore Blvd, St Augustine, FL 32080 USA. EM wharvey@whitney.ufl.edu FU NIH [AI-52436, AI-30464, AI-45098]; American Heart Association [10SDG3830004]; Emerging Pathogens Institute; Whitney Laboratory for Marine Bioscience, University of Florida FX This research was supported in part by NIH Research Grants AI-52436, AI-30464 (WRH) and AI-45098 (PJL), American Heart Association Scientist Development Grant 10SDG3830004 (MAX), by funds from The Emerging Pathogens Institute and The Whitney Laboratory for Marine Bioscience, University of Florida and by a gift from Mrs. Barbara Mayer of Storrs, CT, We thank Dr. Michael J. Adang from the University of Georgia for the antibody to APN2 and Ms. M. Lynn Milstead for the artwork and assistance with the figures. NR 30 TC 1 Z9 1 U1 3 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD APR PY 2012 VL 58 IS 4 SI SI BP 580 EP 589 DI 10.1016/j.jinsphys.2012.01.001 PG 10 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 932JD UT WOS:000303286400019 PM 22251673 ER PT J AU Hoberg, EP Abrams, A Pilitt, PA Kutz, SJ AF Hoberg, Eric P. Abrams, Arthur Pilitt, Patricia A. Kutz, Susan J. TI DISCOVERY AND DESCRIPTION OF THE "DAVTIANI" MORPHOTYPE FOR TELADORSAGIA BOREOARCTICUS (TRICHOSTRONGYLOIDEA: OSTERTAGIINAE) ABOMASAL PARASITES IN MUSKOXEN, OVIBOS MOSCHATUS, AND CARIBOU, RANGIFER TARANDUS, FROM THE NORTH AMERICAN ARCTIC: IMPLICATIONS FOR PARASITE FAUNAL DIVERSITY SO JOURNAL OF PARASITOLOGY LA English DT Article ID SUB-SAHARAN AFRICA; NEMATODA TRICHOSTRONGYLOIDEA; CIRCUMCINCTA NEMATODA; HELMINTH-PARASITES; CLIMATE-CHANGE; LOPEZ-NEYRA; GEN. NOV; POPULATION; RUMINANTS; SHEEP AB Collections to explore helminth diversity among free-ranging ungulates in the North American Arctic revealed the occurrence of a third male, or "davtiani," morphotype for Teladorsagia boreoarcticus. Designated as T. boreoarcticus forma (f.) minor B, the males occurred with T. boreoarcticus f. major and T borearcticus f. minor A in endemic populations of muskoxen (Ovibos moschatus wardi) and barrenground caribou (Rangifer tarandus groenlandicus) on Victoria Island, Nunavut, Canada, and in muskoxen and Peary caribou (Rangifer tarandus pearyi) on Banks Island, Northwest Territories, Canada. These specimens differ from conspecific morphotypes in the structure of the genital cone and Sjoberg's organ. Relative to T. boreoarcticus f. minor A, specimens of T. boreoarcticus f. minor B are consistently smaller, and mean dimensions for the bursa and spicules do not overlap. The robust spicules are similar in form, particularly in the relative length of the dorsal and ventral processes, but mean total length is substantially less in specimens of T. boreoarcticus f. minor B. Differences that distinguish the minor morphotypes of T. boreoarcticus parallel those demonstrated for the T. trifurcata and T. davtiani morphotypes in association with T. circumcincta sensu stricto. New host and geographic records include the 3 morphotypes of T. boreoarcticus in muskoxen and Peary caribou from Banks Island and in barrenground caribou from Victoria Island. Recognition of the ubiquitous nature of cryptic species emphasizes the need to effectively develop and use our collections-based resources and museum archives to build a robust understanding of the biosphere. Field inventory should include provisions for integrative approaches that preserve specimens suitable for comparative morphology, multifaceted molecular investigations, and population genetics. C1 [Hoberg, Eric P.; Abrams, Arthur; Pilitt, Patricia A.; Kutz, Susan J.] Agr Res Serv, US Natl Parasite Collect & Anim Parasit Dis Lab, USDA, BARC E, Beltsville, MD 20705 USA. RP Hoberg, EP (reprint author), Agr Res Serv, US Natl Parasite Collect & Anim Parasit Dis Lab, USDA, BARC E, Bldg 1180,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM eric.hoberg@ars.usda.gov FU National Science Foundation [DEB 0196095, 0415668] FX We gratefully acknowledge Dr. John Nagy, and Brett Elkin (Government of the NWT), and Damian Panayi (Golder Associates) for field collections that resulted in specimens documented here. Laboratory procedures were completed with assistance by Rekha Trembath, Liz Gordon, and Farhad Ghandi (University of Calgary). We thank Judith Price and Canadian Museum of Nature for access to specimens from Ellesmere Island. This study is a contribution of the Beringian Coevolution Project supported in part by the National Science Foundation (DEB 0196095 and 0415668). NR 45 TC 3 Z9 4 U1 3 U2 13 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD APR PY 2012 VL 98 IS 2 BP 355 EP 364 DI 10.1645/GE-2898.1 PG 10 WC Parasitology SC Parasitology GA 932FL UT WOS:000303275700020 PM 21942428 ER PT J AU Alvarado-Esquivel, C Gonzalez-Salazar, AM Alvarado-Esquivel, D Ontiveros-Vazquez, F Vitela-Corrales, J Villena, I Dubey, JP AF Alvarado-Esquivel, C. Gonzalez-Salazar, A. M. Alvarado-Esquivel, D. Ontiveros-Vazquez, F. Vitela-Corrales, J. Villena, I. Dubey, J. P. TI Seroprevalence of Toxoplasma gondii Infection in Chickens in Durango State, Mexico SO JOURNAL OF PARASITOLOGY LA English DT Article ID AVIAN TOXOPLASMOSIS AB Little is known concerning the seroprevalence of Toxoplasma gondii infection in chickens (Gallus domesticus) in Mexico. Antibodies to T. gondii were determined in 519 chickens in Durango, Mexico using the modified agglutination test (MAT). Two groups (A, B) of chickens were sampled. Group A chickens (n = 51) were raised in backyards in 7 municipalities in 3 geographical regions in Durango State. Group B chickens were raised in farms in the Mexican States of Sinaloa (n = 289) and Nayarit (n = 179) but slaughtered in 2 abattoirs in Durango City. Overall, antibodies to T gondii were found in 36 (6.9%) of 519 chickens, with MAT titers of 1:25 in 22, 1:50 in 8, 1:100 in 2, 1:200 in 3, and 1:400 in I. Seroprevalence of T gondii increased significantly with age and was significantly higher in Group A chickens than in Group B chickens. In Group A chickens, a 25.5% seroprevalence of T. gondii infection was found. Seropositive chickens were found in all 7 municipalities sampled. In Group B chickens, the seroprevalence of T. gondii infection was 4.9%. This is the first report of T. gondii infection in chickens in Durango State, Mexico. C1 [Dubey, J. P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Alvarado-Esquivel, C.; Alvarado-Esquivel, D.] Juarez Univ Durango State, Fac Med & Nutr, Durango 34000, Mexico. [Gonzalez-Salazar, A. M.; Ontiveros-Vazquez, F.; Vitela-Corrales, J.] Reg Livestock Union Durango, Lab Anim Pathol, Durango 34217, Mexico. [Villena, I.] Hosp Maison Blanche, Biol Resources Ctr Toxoplasma, Natl Reference Ctr Toxoplasmosis, Lab Parasitol Mycol, F-51092 Reims, France. RP Dubey, JP (reprint author), ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 7 TC 4 Z9 4 U1 0 U2 4 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD APR PY 2012 VL 98 IS 2 BP 431 EP 432 DI 10.1645/GE-2979.1 PG 2 WC Parasitology SC Parasitology GA 932FL UT WOS:000303275700030 PM 22014023 ER PT J AU Baranowski, T Islam, N Baranowski, J Martin, S Beltran, A Dadabhoy, H Adame, SH Watson, KB Thompson, D Cullen, KW Subar, AF AF Baranowski, Tom Islam, Noemi Baranowski, Janice Martin, Shelby Beltran, Alicia Dadabhoy, Hafza Adame, Su-heyla Watson, Kathleen B. Thompson, Debbe Cullen, Karen W. Subar, Amy F. TI Comparison of a Web-Based versus Traditional Diet Recall among Children SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Diet assessment; Computer; 24-hour recall; Children ID 13-YEAR-OLD CHILDREN; FOOD-INTAKE; ACCURACY; NUTRITION; VALIDITY AB Self-administered instruments offer a low-cost diet assessment method for use in adult and pediatric populations. This study tested whether 8- to 13-year-old children could complete an early version of the Automated Self Administered 24-hour diet recall (ASA24) and how this compared to an interviewer-administered 24-hour diet recall. One-hundred twenty 8- to 13-year-old children were recruited in Houston from June through August 2009 and randomly assigned to complete either the ASA24 or an interviewer-administered 24-hour diet recall, followed by the other recall mode covering the same time interval. Multivariate analysis of variance, testing for differences by age, sex, and ethnic/racial group, were applied to percentages of food matches, intrusions, and omissions between reports on the ASA24 and the interviewer-administered 24-hour diet recall. For the ASA24, qualitative findings were reported regarding ease of use. Overall matches between interviewer-administered and ASA24 self-administered 24-hour diet recall was 47.8%. Matches were significantly lower among younger (8- to 9-year-old) compared with older (10- to 13-year-old) children. Omissions on ASA24 (18.9% overall) were most common among 8-year-olds and intermediate among 9-year-olds. Eight- and 9-year-olds had substantial difficulties and often required aid in completing ASA24. Findings from this study suggest that a simpler version of an Internet-based diet recall program would be easier for children to use. J Acad Nutr Diet. 2012;112:527-532. C1 [Baranowski, Tom; Islam, Noemi; Baranowski, Janice; Martin, Shelby; Beltran, Alicia; Dadabhoy, Hafza; Adame, Su-heyla; Watson, Kathleen B.; Thompson, Debbe; Cullen, Karen W.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Subar, Amy F.] NCI, Div Canc Control & Populat Sci, Appl Res Program, Risk Factor Monitoring & Methods Branch EPN, Bethesda, MD 20892 USA. RP Baranowski, T (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222 FU National Cancer Institute [5 U01 CA130762-04]; USDA/ARS [58-6250-6001] FX This research was primarily funded by a grant from the National Cancer Institute (5 U01 CA130762-04). This work is also a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX, and had been funded in part with federal funds from the USDA/ARS under Cooperative Agreement No. 58-6250-6001. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the US government. NR 24 TC 22 Z9 22 U1 0 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD APR PY 2012 VL 112 IS 4 BP 527 EP 532 DI 10.1016/j.jada.2011.10.002 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 930WR UT WOS:000303175900010 PM 22717216 ER PT J AU Baker, CJ Kovalskaya, NY Mock, NM Owens, RA Deahl, KL Whitaker, BD Roberts, DP Hammond, RW Aver'yanov, AA AF Baker, C. Jacyn Kovalskaya, Natalia Y. Mock, Norton M. Owens, Robert A. Deahl, Kenneth L. Whitaker, Bruce D. Roberts, Daniel P. Hammond, Rose W. Aver'yanov, Andrey A. TI An internal standard technique for improved quantitative analysis of apoplastic metabolites in tomato leaves SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Internal standard; Dinotefuran; Phenolics; Apoplast; Redox; Bioactivity; Technique; Tomato ID OXIDATIVE BURST; PHENOLIC OXIDATION; PLANT; PEROXIDASE; ANTIOXIDANTS; DEFENSE; STRESS; FLUID; WATER; ACID AB The plant leaf apoplast is one of the first lines of defense against many foliar pathogens. The aqueous layer lining the airspace within leaves is enriched with secondary metabolites that can serve many roles including protection against environmental hazards, both biotic and abiotic. The constituents and their concentration change as the leaf matures or undergoes stress. To monitor and quantify changes in these metabolites during pathogen stress, we needed a more sensitive technique. We were able to modify the infiltration-centrifugation technique to use smaller samples, individual tomato leaflets, plus use an internal standard which indicated the amount of dilution in each sample. Dinotefuran, a neonicotinoid used as a systemic pesticide, proved to be resistant to the redox environment of the apoplast and had similar chemical properties allowing it to be analyzed under the same UPLC conditions as the other phenolic metabolites. Water soluble metabolites on the leaf surface were found to be a major source of contamination that could be avoided by rinsing leaves with water prior to infiltration. The improved sample efficiency and accuracy provided by this technique, along with the use of Dinotefuran as an internal standard, will provide the sensitivity needed to monitor apoplast metabolites during pathogen stress. Published by Elsevier Ltd. C1 [Baker, C. Jacyn; Mock, Norton M.; Owens, Robert A.; Hammond, Rose W.] USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Whitaker, Bruce D.] USDA, Produce Qual & Safety Lab, Beltsville, MD 20705 USA. [Roberts, Daniel P.] USDA, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Deahl, Kenneth L.] USDA, Vegetable Lab, Beltsville, MD 20705 USA. [Kovalskaya, Natalia Y.] Penn State Univ, Dept Plant Pathol, University Pk, PA 16802 USA. [Aver'yanov, Andrey A.] Res Inst Phytopathol, B Vyazemy 143050, Moscow Region, Russia. RP Baker, CJ (reprint author), USDA, Mol Plant Pathol Lab, Bldg 004,Rm 119, Beltsville, MD 20705 USA. EM jacyn.baker@ars.usda.gov NR 32 TC 7 Z9 7 U1 2 U2 14 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2012 VL 78 BP 31 EP 37 DI 10.1016/j.pmpp.2012.01.001 PG 7 WC Plant Sciences SC Plant Sciences GA 930AA UT WOS:000303106400005 ER PT J AU McCulloh, KA Woodruff, DR AF McCulloh, Katherine A. Woodruff, David R. TI Linking stomatal sensitivity and whole-tree hydraulic architecture SO TREE PHYSIOLOGY LA English DT Article ID VAPOR-PRESSURE DEFICIT; DIFFUSE-POROUS TREES; BETULA-OCCIDENTALIS; WATER TRANSPORT; URBAN TREES; CONDUCTANCE; TRANSPIRATION; CAVITATION; RESPONSES; FOREST C1 [McCulloh, Katherine A.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Woodruff, David R.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR USA. RP McCulloh, KA (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM kate.mcculloh@oregonstate.edu FU National Sciences Foundation [IBN 09-19871] FX This commentary was supported by the National Sciences Foundation (IBN 09-19871). NR 26 TC 5 Z9 7 U1 0 U2 24 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD APR PY 2012 VL 32 IS 4 BP 369 EP 372 DI 10.1093/treephys/tps036 PG 4 WC Forestry SC Forestry GA 933DQ UT WOS:000303340000001 PM 22544047 ER PT J AU Cane, JH Weber, M Miller, S AF Cane, James H. Weber, Melissa Miller, Stephanie TI BREEDING BIOLOGIES, POLLINATORS, AND SEED BEETLES OF TWO PRAIRIE-CLOVERS, DALEA ORNATA AND DALEA SEARLSIAE (FABACEAE: AMORPHEAE), FROM THE INTERMOUNTAIN WEST, USA SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID REPRODUCTIVE SUCCESS; PURPUREA FABACEAE AB Two prairie-clovers, Dalea ornata (Douglas ex Hook.) Eaton & J. Wright and Dalea searlsiae (A. Gray) Barneby, are perennial forbs found sporadically in the U.S. Intermountain West. Their seed is desirable for use in range-land restoration. We experimentally characterized the breeding biologies of D. ornate and D. searlsiae in a common garden, surveyed their pollinator guilds, and sampled their seed predators. The 2 Dalea species, being primarily xenogamous, have comparable pollination requirements. For flowers manually pollinated with outcross pollen, an average of 42% of D. ornata flowers and 39% of D. searlsiae flowers yielded plump large seeds filled with endosperm. Both species proved to be self-compatible, but far fewer seeds resulted from either manual pollination with self-pollen (11% seed set for D. ornata and 7% for D. searlsiae) or unassisted autogamy (5% and 6% seed set, respectively). Limited surveys of the prairie-clovers pollinator guilds in ruderal or cheatgrass-infested habitats revealed sparse visitation solely by wild bees, primarily of the genera Anthidium, Colletes, Bombus, Eucera, and Melissodes. Beetles (Acanthoscelides oregonensis Johnson and Apion amaurum Kissinger) infested seed sampled from 18 of 25 D. ornata populations across a 3-state region. Productive farming of the seed of these prairie-clovers for rangeland restoration in the western United States will require supplementation of bees for pollination and exclusion of seed beetles. C1 [Cane, James H.; Weber, Melissa; Miller, Stephanie] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. [Weber, Melissa] Jacobs Engn Grp Inc, Salt Lake City, UT 84103 USA. [Miller, Stephanie] Oregon Dept Fish & Wildlife, Corvallis, OR 97333 USA. RP Cane, JH (reprint author), Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. EM jim.cane@ars.usda.gov FU USDI-BLM Great Basin Restoration Initiative; USDA Forest Service Rocky Mountain Research Station FX Kevin Connors and Douglas Johnson kindly gave us access to their seed collections, which we examined for seed predators. The Acanthoscelides weevils were expertly identified by Jesus Napoles and the late Clarence Johnson. Byron Love, Kristal Watrous, and Linda Kervin assisted with some of the bee collections. This research was funded by the Great Basin Native Seed Selection and Increase Project through the USDI-BLM Great Basin Restoration Initiative and the USDA Forest Service Rocky Mountain Research Station. We are grateful to Douglas Johnson, Shaun Bushman, and 2 anonymous reviewers for detailed, constructive reviews. NR 14 TC 3 Z9 3 U1 0 U2 15 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD APR PY 2012 VL 72 IS 1 BP 16 EP 20 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 930KU UT WOS:000303139100002 ER PT J AU Sowerwine, JE Rinella, MJ Carlson, ML AF Sowerwine, James E. Rinella, Matthew J. Carlson, Matthew L. TI DO POPULATIONS OF AN INVASIVE WEED DIFFER GREATLY IN THEIR PER-GRAM COMPETITIVE EFFECTS? SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID PLANT-COMMUNITIES; IMPACT; DYNAMICS; MODEL; DIFFERENTIATION; ABUNDANCE; SELECTION; DENSITY; TRAITS AB Quantifying an invasive species negative impacts across its introduced range will be quite challenging if the impacts vary unpredictably from site to site or from population to population. Little emphasis, however, has been placed on quantifying such interpopulation variation in the impacts of individual invasive species. We studied the response of a native grass (Festuca rubra) to competition from 4 geographically dispersed invasive plant (Melilotus albus) populations in order to determine if some populations of this invader have greater competitive impacts than others. Despite the relatively large number of experimental units in our greenhouse study, we did not obtain evidence that competitive effects per gram of biomass varied by invader population. Therefore, in some cases it should be possible to estimate the effects of invasive weeds with simple competition models that ignore some forms of phenotypic variation, as long as the models control for invader biomass per unit area (i.e., invader yield). C1 [Rinella, Matthew J.] USDA ARS, Ft Keogh LAARL, Miles City, MT 59301 USA. [Sowerwine, James E.] Univ Alaska, Dept Biol Sci, Anchorage, AK 99508 USA. [Carlson, Matthew L.] Univ Alaska, Dept Biol Sci, Anchorage, AK 99501 USA. [Carlson, Matthew L.] Univ Alaska, Alaska Nat Heritage Program, Anchorage, AK 99501 USA. RP Rinella, MJ (reprint author), USDA ARS, Ft Keogh LAARL, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM matt.rinella@ars.usda.gov FU Alaska EPSCoR (NSF and Alaska State) [EPS-0701898]; UAA FX Chad Cook, Jeff Conn, Jill Yarbrough, Katie Villiano, and the Alaska Plant Materials Center assisted with seed collections. The University of Alaska Anchorage Engineering Department supplied greenhouse space. Jeff Conn and Steve Seefeldt provided valuable input on drafts of the manuscript. We gratefully acknowledge support from the Alaska EPSCoR (NSF and Alaska State award #EPS-0701898) and the Alaska Natural Heritage Program, UAA. NR 33 TC 0 Z9 0 U1 0 U2 9 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD APR PY 2012 VL 72 IS 1 BP 43 EP 47 PG 5 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 930KU UT WOS:000303139100005 ER PT J AU Rinella, MJ Dean, R Vavra, M Parks, CG AF Rinella, Matthew J. Dean, Ron Vavra, Marty Parks, Catherine G. TI VEGETATION RESPONSES TO SUPPLEMENTAL WINTER FEEDING OF ELK IN WESTERN WYOMING SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID YELLOWSTONE-NATIONAL-PARK; FREE-RANGING ELK; COMMUNITIES; INVASIBILITY; BRUCELLOSIS; DISTURBANCE; ASPEN; SEED AB To compensate for losses in overwintering habitat, elk are fed hay in winter at approximately 37 locations throughout the western United States. These winter feeding programs concentrate elk activity and there is concern that such concentrations could degrade plant communities. Except for one study focused exclusively on aspen (Populus tremuloides), ours is the first to quantify vegetation responses to supplemental winter feeding of elk. The western Wyoming feedground we studied was established in winter feed-ground, and supplemental feeding occurred every winter through 2006. Transects were arranged in a before-after control-impact (BACI) design, and vegetation data were gathered in the mid-summers of 1981 and 2006. Over the study period, the feedground became invaded by smooth brome (Bromus inermis), a nonnative grass constituent of hay fed to the elk. The smooth brome invasion was attended by declines in native forb cover and the apparent extirpation of shrubs from the feedground. The smooth brome invasion did not extend beyond the feedground, but an area 125 m from the feedground showed decreases in shrubs. At areas >= 750 m from the feedground, forbs and shrubs did not decrease demonstrably, and in some cases they showed evidence of increasing over the study period. Supplemental winter feeding at a feedground in western Wyoming degraded the plant community, but, with the possible exception of aspen, the degradation did not appear to extend great distances beyond the small area where the animals were fed. C1 [Rinella, Matthew J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Dean, Ron] Wyoming Dept Game & Fish, Alpine, WY 83128 USA. [Vavra, Marty; Parks, Catherine G.] US Forest Serv, USDA, Pacific NW Res Stn, La Grande, OR 97850 USA. RP Rinella, MJ (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM matt.rinella@ars.usda.gov NR 21 TC 4 Z9 4 U1 1 U2 24 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD APR PY 2012 VL 72 IS 1 BP 78 EP 83 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 930KU UT WOS:000303139100009 ER PT J AU Rosen, H AF Rosen, Howard TI WHAT IS WOOD AND FOREST CULTURE RESEARCH AND DOES IT MATTER? SO WOOD AND FIBER SCIENCE LA English DT Editorial Material C1 US Forest Serv, IUFRO Wood Culture Working Party, Morgantown, WV USA. RP Rosen, H (reprint author), US Forest Serv, IUFRO Wood Culture Working Party, Morgantown, WV USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU SOC WOOD SCI TECHNOL PI MADISON PA ONE GIFFORD PINCHOT DR, MADISON, WI 53705 USA SN 0735-6161 J9 WOOD FIBER SCI JI Wood Fiber Sci. PD APR PY 2012 VL 44 IS 2 BP 119 EP 120 PG 2 WC Forestry; Materials Science, Paper & Wood; Materials Science, Textiles SC Forestry; Materials Science GA 931PM UT WOS:000303231800001 ER PT J AU Drenovsky, RE Khasanova, A James, JJ AF Drenovsky, Rebecca E. Khasanova, Albina James, Jeremy J. TI TRAIT CONVERGENCE AND PLASTICITY AMONG NATIVE AND INVASIVE SPECIES IN RESOURCE-POOR ENVIRONMENTS SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE forbs; gas exchange; Great Basin; knapweed; nitrogen; resorption proficiency ID RELATIVE GROWTH-RATE; WATER-USE EFFICIENCY; PHENOTYPIC PLASTICITY; NUTRIENT RESORPTION; NITROGEN-FERTILIZATION; PLANT INVASION; LEAF TRAITS; COMMUNITY; ECOPHYSIOLOGY; METAANALYSIS AB Premise of study: Functional trait comparisons provide a framework with which to assess invasion and invasion resistance. However, recent studies have found evidence for both trait convergence and divergence among coexisting dominant native and invasive species. Few studies have assessed how multiple stresses constrain trait values and plasticity, and no study has included direct measurements of nutrient conservation traits, which are critical to plants growing in low-resource environments. Methods: We evaluated how nutrient and water stresses affect growth and allocation, water potential and gas exchange, and nitrogen (N) allocation and use traits among a suite of six codominant species from the Intermountain West to determine trait values and plasticity. In the greenhouse, we grew our species under a full factorial combination of high and low N and water availability. We measured relative growth rate (RGR) and its components, total biomass, biomass allocation, midday water potential, photosynthetic rate, water-use efficiency (WUE), green leaf N, senesced leaf N, total N pools, N productivity, and photosynthetic N use efficiency. Key results: Overall, soil water availability constrained plant responses to N availability and was the major driver of plant trait variation in our analysis. Drought decreased plant biomass and RGR, limited N conservation, and led to increased WUE. For most traits, native and nonnative species were similarly plastic. Conclusions: Our data suggest native and invasive biomass dominants may converge on functionally similar traits and demonstrate comparable ability to respond to changes in resource availability. C1 [Drenovsky, Rebecca E.; Khasanova, Albina] John Carroll Univ, Dept Biol, University Hts, OH 44118 USA. [James, Jeremy J.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Drenovsky, RE (reprint author), John Carroll Univ, Dept Biol, 20700 N Pk Blvd, University Hts, OH 44118 USA. EM rdrenovsky@jcu.edu FU John Carroll University FX The authors thank L. Ziegenhagen for laboratory and greenhouse assistance and two anonymous reviewers for helpful comments on the manuscript. A Summer Research Fellowship from John Carroll University (R.E.D.) helped support this work. NR 61 TC 26 Z9 26 U1 5 U2 68 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD APR PY 2012 VL 99 IS 4 BP 629 EP 639 DI 10.3732/ajb.1100417 PG 11 WC Plant Sciences SC Plant Sciences GA 926DB UT WOS:000302810400015 PM 22434772 ER PT J AU Ballesteros, D Estrelles, E Walters, C Ibars, AM AF Ballesteros, Daniel Estrelles, Elena Walters, Christina Ibars, Ana M. TI EFFECTS OF TEMPERATURE AND DESICCATION ON EX SITU CONSERVATION OF NONGREEN FERN SPORES SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE aging; cryopreservation; ferns; freeze damage; gametophyte development; germination; germplasm; lipids; longevity; long-term storage ID EARLY GAMETOPHYTE DEVELOPMENT; ENDANGERED TREE FERN; CALORIMETRIC PROPERTIES; STORAGE-TEMPERATURE; SEED LONGEVITY; VIABILITY; GERMINATION; TRIACYLGLYCEROLS; CRYOPRESERVATION; WATER AB Premise of the study: Fern spores are unicellular and haploid, making them a potential model system to study factors that regulate lifespan and mechanisms of aging. Aging rates of nongreen spores were measured to compare longevity characteristics among diverse fern species and test for orthodox response to storage temperature and moisture. Methods: Aging of spores from 10 fern species was quantified by changes in germination and growth parameters. Storage temperature ranged from ambient room to -196 degrees C (liquid nitrogen); spores were dried to ambient relative humidity (RH) or using silica gel. Key results: Survival of spores varied under ambient storage conditions, with one species dying within a year and two species having greater than 50% survival after 3 years. Few changes in germination or growth were observed in spores stored at either -80 degrees C or -196 degrees C over the same 3-yr study period. Spores stored at -25 degrees C aged anomalously quickly, especially those dried to ambient RH or subjected to repeated freeze-thaw cycles. Conclusions: Spore longevity is comparable to orthodox seed longevity under ambient storage conditions, with wide variation among species and shelflife extended by drying or cooling. However, faster aging during freezer storage may indicate a similar syndrome of damage experienced by seeds categorized as "intermediate". The damage is avoided by storage at -80 degrees C or liquid nitrogen temperatures, making cryoconservation an effective and broadly applicable tool to extend fern spore longevity. The study demonstrates that spore banks are a feasible approach for ex situ conservation of this important plant group. C1 [Ballesteros, Daniel; Estrelles, Elena; Ibars, Ana M.] Jardi Bot Univ Valencia ICBiBE, Banco Germoplasma, Valencia 46008, Spain. [Ballesteros, Daniel; Walters, Christina] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. RP Ballesteros, D (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, 1111 S Mason St, Ft Collins, CO 80521 USA. EM daniel.ballesteros@uv.es RI Estrelles-Perpina, Elena/G-5152-2016; Ballesteros, Daniel/A-8947-2017; OI Estrelles, Elena/0000-0002-2869-3684 FU FPU from the Spanish Ministry of Science and Technology; Ministry of Education and Science of Spain [REN 2002-03697 / GLO] FX The authors thank Pat Reeves for thoughtful comments and for editing the manuscript. D.B. was supported by an FPU grant from the Spanish Ministry of Science and Technology. This work was funded by the Ministry of Education and Science of Spain (REN 2002-03697 / GLO). Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 50 TC 11 Z9 16 U1 5 U2 39 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD APR PY 2012 VL 99 IS 4 BP 721 EP 729 DI 10.3732/ajb.1100257 PG 9 WC Plant Sciences SC Plant Sciences GA 926DB UT WOS:000302810400024 PM 22434770 ER PT J AU Hanberry, BB Yang, J Kabrick, JM He, HS AF Hanberry, Brice B. Yang, Jian Kabrick, John M. He, Hong S. TI Adjusting Forest Density Estimates for Surveyor Bias in Historical Tree Surveys SO AMERICAN MIDLAND NATURALIST LA English DT Article ID SURVEY RECORDS; LAND SURVEY; PRESETTLEMENT; MICHIGAN AB The U.S. General Land Office surveys, conducted between the late 1700s to early 1900s, provide records of trees prior to widespread European and American colonial settlement. However, potential and documented surveyor bias raises questions about the reliability of historical tree density estimates and other metrics based on density estimated from these records. In this study, we present two complementary approaches to adjust density estimates for possible surveyor bias. We addressed the problem of surveyor bias of density estimates by simulating the effects of (1) rank of selected trees (compared to assuming the nearest trees were selected) and (2) specific surveyor bias in selection of (a) quadrant location, (b) quadrant configuration, (c) azimuth, and (d) combined species and diameter. We then developed regression equations to calculate adjustment quotients for these biases, making the adjustment quotients transferable to any similar datasets. For the rank-based approach, an unvarying rank of 2 (selection of the second nearest tree instead of always the nearest tree) decreased density estimates to about 25 to 45% of the actual density, depending on number of trees per survey point, resulting in corrected density estimates that are 2.2 to 4 times greater than uncorrected density estimates. However, constant selection of the second nearest tree did not occur; varying ranks decreased density estimates to around 55 to 65% of the density, resulting in corrected density estimates about 1.5 to 1.8 times greater than uncorrected values. For the bias-based approach, depending on the specific General Land Office dataset, bias for tree species and diameter alone may decrease density estimates by about 35%. Quadrant configuration and azimuth preference may decrease density estimates by about 15% each. The quadrant location bias has negligible effects on the density estimates. The overall density estimates may be about 35 to 55% of the actual density and correction of the density estimate will approximately double the value. These methods can provide a range of estimates, from low values of uncorrected density to high values of corrected density, about the amount that varying surveyor bias may have decreased density estimates for any areas where bias is detected (i.e., non-random frequencies) in point-centered quarter surveys. Adjustments will increase reliability of historical forest density estimates and their applications for restoration. C1 [Hanberry, Brice B.; He, Hong S.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Yang, Jian] Chinese Acad Sci, Inst Appl Ecol, Shenyang 110016, Peoples R China. [Kabrick, John M.] Univ Missouri, No Res Stn, USDA Forest Serv, Columbia, MO 65211 USA. RP Hanberry, BB (reprint author), Univ Missouri, Dept Forestry, 203 Nat Resources Bldg, Columbia, MO 65211 USA. EM hanberryb@missouri.edu RI Yang, Jian/H-3169-2011; OI Yang, Jian/0000-0002-2170-589X; He, Hong S./0000-0002-3983-2512 FU National Fire Plan; USDA Forest Service, Northern Research Station FX We thank William Dijak for programming assistance and reviewers for helpful suggestions. Support was provided by the National Fire Plan and the USDA Forest Service, Northern Research Station. NR 17 TC 14 Z9 14 U1 0 U2 6 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 J9 AM MIDL NAT JI Am. Midl. Nat. PD APR PY 2012 VL 167 IS 2 BP 285 EP 306 PG 22 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 925IT UT WOS:000302755600006 ER PT J AU Kovendan, K Murugan, K Vincent, S Barnard, DR AF Kovendan, K. Murugan, K. Vincent, S. Barnard, Donald R. TI Mosquito larvicidal properties of Orthosiphon thymiflorus (Roth) Sleesen. (Family: Labiatae) against mosquito vectors, Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae) SO ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE LA English DT Article DE Orthosiphon thymiflorus; Anopheles stephensi; Aedes aegypti; Culex quinquefasciatus; Larvicidal activity ID MALARIAL VECTOR; EXTRACTS; RESISTANCE; REPELLENT; STAMINEUS; EFFICACY; LARVAE; VITEX; L. AB Objective: To determine the mosquito larvicidal activities of hexane, chloroform, ethyl acetate, acetone and methanol leaf extract of Orthosiphon thymiflorus (O. thymiflorus) against Anopheles stephensi (An. stephensi), Culex quinquefasciatus (Cx. quinquefasciatus) and Aedes aegypti (Ae. aegypti). Methods: The larvicidal activity was assayed against three mosquito species at various concentrations ranging from (50-450 ppm) under the laboratory conditions. The LC50 and LC90 of the O. thymiflorus leaf extract was determined by Probit analysis. Results: The LC50 values of hexane, chloroform, ethyl acetate, acetone and methanol extract of O. thymiflorus third instar larvae of An. stephensi were LC50= 201.39, 178.76, 158.06, 139.22 and 118.74 ppm; Cx. quinquefasciatus were LC50=228.13, 209.72, 183.35, 163.55 and 149.96 ppm and Ae. aegypti were LC50=215.65, 197.91, 175.05, 154.80 and 137.26 ppm, respectively. Maximum larvicidal activity was observed in the methanolic extract followed by acetone, ethyl acetate chloroform and hexane extract. The larval mortality was observed after 24 h exposure. No mortality was observed in control. Conclusions: The present results suggest that the effective plant crude extracts have potential to be used as an ideal eco-friendly approach for the control of mosquito vectors. This study provides the first report on the larvicidal activity of this plant crude solvent extract of against An. stephensi, ex. quinquefasciatus and Ae. aegypti mosquitoes. C1 [Kovendan, K.; Murugan, K.] Bharathiar Univ, Div Entomol, Dept Zool, Sch Life Sci, Coimbatore 641046, Tamil Nadu, India. [Vincent, S.] Loyola Coll, PG Res & Dept Adv Zool & Biotechnol, Madras 600034, Tamil Nadu, India. [Barnard, Donald R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Kovendan, K (reprint author), Bharathiar Univ, Div Entomol, Dept Zool, Sch Life Sci, Coimbatore 641046, Tamil Nadu, India. EM gokulloyo@yahoo.co.in FU Department of Science and Technology (DST), Govt. of India New Delhi, India; Tamil Nadu State Council for Science and Technology (TNSCST), Chennai, Tamil Nadu FX The authors are thankful to the Department of Science and Technology (DST), Govt. of India New Delhi, India and Tamil Nadu State Council for Science and Technology (TNSCST), Chennai, Tamil Nadu for providing financial support for the present work. The authors are grateful to the Dr. K. Sasikala, Professor and Head, Department of Zoology, Bharathiar University for the laboratory facilities provided. NR 48 TC 9 Z9 10 U1 1 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1995-7645 J9 ASIAN PAC J TROP MED JI Asian Pac. J. Trop. Med. PD APR PY 2012 VL 5 IS 4 BP 299 EP 305 PG 7 WC Public, Environmental & Occupational Health; Tropical Medicine SC Public, Environmental & Occupational Health; Tropical Medicine GA 926YP UT WOS:000302869700009 PM 22449522 ER PT J AU Cushman, RA AF Cushman, Robert A. TI Evidence That the Autoimmune Regulator Gene Influences Thymic Production of Ovarian Antigens and Prevents Autoimmune-Mediated Premature Reproductive Senescence SO BIOLOGY OF REPRODUCTION LA English DT Editorial Material ID ANTRAL FOLLICLE COUNT; AIRE C1 USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Cushman, RA (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM bob.cushman@ars.usda.gov NR 17 TC 2 Z9 2 U1 0 U2 0 PU SOC STUDY REPRODUCTION PI MADISON PA 1603 MONROE ST, MADISON, WI 53711-2021 USA SN 0006-3363 J9 BIOL REPROD JI Biol. Reprod. PD APR PY 2012 VL 86 IS 4 AR 109 DI 10.1095/biolreprod.112.099242 PG 2 WC Reproductive Biology SC Reproductive Biology GA 925NG UT WOS:000302767300012 PM 22278984 ER PT J AU Scordia, D Cosentino, SL Lee, JW Jeffries, TW AF Scordia, Danilo Cosentino, Salvatore L. Lee, Jae-Won Jeffries, Thomas W. TI Bioconversion of giant reed (Arundo donax L.) hemicellulose hydrolysate to ethanol by Scheffersomyces stipitis CBS6054 SO BIOMASS & BIOENERGY LA English DT Article; Proceedings Paper CT Conference and Exhibition on Biomass for Energy (WORLD BIOENERGY) CY MAY 29-31, 2012 CL Jonkoping, SWEDEN DE Arundo donax; Giant reed; Oxalic acid pretreatment; Hemicellulose hydrolysate; Bioethanol production; Scheffersomyces (Pichia) stipitis ID OXALIC-ACID PRETREATMENT; PICHIA-STIPITIS; ENERGY CROPS; CORN STOVER; FERMENTATION; XYLOSE; YEASTS; HYDROLYZATE; INHIBITORS; LIGNOCELLULOSICS AB The objective of this study was to evaluate the production of ethanol by Scheffersomyces (Pichia) stipitis CBS6054, a native xylose fermenting yeast, from sugars contained in the giant reed (Arundo donax L.) hemicellulosic hydrolysate. A response surface methodology with two input parameters, severity factor and oxalic acid concentration ranging from 2.87 to 4.05 and from 2 to 8 (% w oxalic acid/w solid dry matter), respectively, was employed to minimize degradation products and maximize sugar release. However, at the optimum condition for sugar release (43.8 gl (1)), levels of toxic degradation products (acetic acid, furfural, HMF and phenolic compounds) were considered too high for yeast fermentation. The condition to minimize degradation products and maximize sugar yields was judged to be 2.87 severity factor and 5.0% oxalic acid concentration. At this condition 26.0 gl (1) xylose, 5.0 gl (1) glucose and 2.4 gl (1) arabinose were recovered in giant reed hydrolysate fraction. Adjustment of pH to 5.0 with Ca(OH)(2) decreased xylose, glucose and acetic acid, 22%, 8% and 27% respectively. Increasing the initial pH from 5.0 to 5.5, 6.0 and 6.5, respectively, significantly improved the fermentability of the giant reed hemicelluloses hydrolysate; no fermentation was observed at pH 5.0 after 96h, while 8.20gl (1) of ethanol was obtained at pH 6.0 after 48 h, with an ethanol yield of 0.33 (g(e)/g(s)) and a productivity of 0.17 gl (1)h (1). The optimum pH of acid hydrolysate fermentation for ethanol production was 6.0-6.5. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Scordia, Danilo; Cosentino, Salvatore L.] Univ Catania, Dipartimento Sci Prod Agr & Alimentari DISPA, I-95123 Catania, Italy. [Lee, Jae-Won] Chonnam Natl Univ, Dept Forest Prod & Technol, BK Program 21, Kwangju 500757, South Korea. [Lee, Jae-Won] Chonnam Natl Univ, Bioenergy Res Ctr, Kwangju 500757, South Korea. [Jeffries, Thomas W.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53705 USA. [Jeffries, Thomas W.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Scordia, D (reprint author), Univ Catania, Dipartimento Sci Prod Agr & Alimentari DISPA, Via Valdisavoia 5, I-95123 Catania, Italy. EM dscordia@unict.it RI Jeffries, Thomas/I-8576-2012; Cosentino, Salvatore/F-7318-2011; OI Jeffries, Thomas/0000-0001-7408-4065; Cosentino, Salvatore/0000-0001-7076-8777; Scordia, Danilo/0000-0002-3822-788X NR 35 TC 40 Z9 42 U1 0 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD APR PY 2012 VL 39 SI SI BP 296 EP 305 DI 10.1016/j.biombioe.2012.01.023 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 926KM UT WOS:000302829900037 ER PT J AU Wang, ML Raymer, P Chinnan, M Pittman, RN AF Wang, Ming Li Raymer, Paul Chinnan, Manjeet Pittman, Roy N. TI Screening of the USDA peanut germplasm for oil content and fatty acid composition SO BIOMASS & BIOENERGY LA English DT Article; Proceedings Paper CT Conference and Exhibition on Biomass for Energy (WORLD BIOENERGY) CY MAY 29-31, 2012 CL Jonkoping, SWEDEN DE Peanut (Arachis hypogaea L.) germplasm; Soybean (Glycine max L.); Variability; Oil content; Methyl esters; Biodiesel production ID BIODIESEL PRODUCTION; FUEL PROPERTIES; MICROALGAE AB Biodiesel can be produced by transesterification of plant oils. The oil content and fatty acid composition of fifty peanut accessions and two soybean accessions were analyzed and compared. In comparison of the oil content, peanut seeds contain a much higher proportion of oil (51.9% of the total seed mass) than soybean seeds (23.3%). Significant variability in oil content (45.9%-55.4%) was detected among peanut accessions. In comparison of the fatty acid composition, peanut seeds contain much higher proportions of oleic acid (43.2%) and behenic acid (3.2%) than soybean seeds (28.8% and 0.3%), and much lower proportions of linoleic acid (35.0%) and linolenic acid (0.1%) than soybean seeds (49.1% and 6.3%). The major differences in fatty acid composition imply fewer double bonds present in peanut oil than in soybean oil. In other words, peanut oil is less susceptible to oxidation than soybean oil. Significant variability in oleic acid (37.0%-55.6%), linoleic acid (25.2%-39.7%), palmitic acid (8.2%-13.8%), and stearic acid (1.7%-6.5%) was detected among peanut accessions. Significant negative correlations of oleic acid with other major fatty acids were detected. Our results indicated that higher oil content and better fatty acid composition of peanut seeds make it a better oilseed crop for biodiesel production. Moreover, based on the variability detected in oil content and fatty acid composition among peanut accessions, there is potential to increase oil content and alter fatty acid composition through peanut breeding. Published by Elsevier Ltd. C1 [Wang, Ming Li; Pittman, Roy N.] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Raymer, Paul] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. [Chinnan, Manjeet] Univ Georgia, Dept Food Sci & Technol, Griffin, GA 30223 USA. RP Wang, ML (reprint author), USDA ARS, Plant Genet Resources Conservat Unit, 1109 Expt St, Griffin, GA 30223 USA. EM mingli.wang@ars.usd.gov NR 17 TC 15 Z9 16 U1 0 U2 23 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD APR PY 2012 VL 39 SI SI BP 336 EP 343 DI 10.1016/j.biombioe.2012.01.025 PG 8 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 926KM UT WOS:000302829900041 ER PT J AU Haas, MS Beeman, RW AF Haas, Merrilee Susan Beeman, Richard W. TI Coming apart at the seams: morphological evidence for pregnathal head capsule borders in adult Tribolium castaneum SO DEVELOPMENT GENES AND EVOLUTION LA English DT Article DE Tribolium castaneum; Head development; Procephalon; Suture; Labrum; Acron ID INTERCALARY SEGMENT; BEETLE TRIBOLIUM; INSECT HEAD; DROSOPHILA; GENE; EXPRESSION; LABRUM; COMPLEX; ORIGIN; ONYCHOPHORAN AB Cephalization and seamless fusion of the anterior body segments during development obscure the segmental boundaries of the insect head. Most of the visible seams are thought to reflect cuticular infolding for structural reinforcement rather than a merger of cuticular plate borders. Incomplete fusions and other modifications of the adult head found in eight Tribolium mutations indicate that the frontal and gular sutures likely are true sutures that mark borders between adjacent cuticular plates, and suggest that the anterior facial shelf is a composite of three independent cuticular surfaces: ocular, antennal, and clypeo-labral. Additionally, midline splits of the clypeo-labrum and gula, and membranous lesions on the lateral head capsule reveal probable borders of adjacent cuticular plates where visible sutures are normally absent. The anterior lateral lesions seen in the Lucifer mutation mark a border between ocular and antennal plates and appear to identify part of the postfrontal sutures. While revealing or clarifying possible intersegmental borders between ocular, antennal, and clypeo-labral plates, the various modified or unfused surfaces of the head neither reveal an additional acronal plate nor support the view that the clypeo-labrum is segmentally associated with ocular cuticle. C1 [Haas, Merrilee Susan; Beeman, Richard W.] ARS, Ctr Grain & Anim Hlth Res, USDA ARS CGAHR, Manhattan, KS 66502 USA. RP Beeman, RW (reprint author), ARS, Ctr Grain & Anim Hlth Res, USDA ARS CGAHR, 1515 Coll Ave, Manhattan, KS 66502 USA. EM mshaas@twinvalley.net; richard.beeman@ars.usda.gov NR 39 TC 0 Z9 0 U1 1 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0949-944X J9 DEV GENES EVOL JI Dev. Genes Evol. PD APR PY 2012 VL 222 IS 2 BP 99 EP 111 DI 10.1007/s00427-012-0397-5 PG 13 WC Cell Biology; Evolutionary Biology; Developmental Biology SC Cell Biology; Evolutionary Biology; Developmental Biology GA 926FF UT WOS:000302816000004 PM 22466423 ER PT J AU Wienhold, BJ Taylor, DB AF Wienhold, B. J. Taylor, D. B. TI Substrate Properties of Stable Fly (Diptera: Muscidae) Developmental Sites Associated With Round Bale Hay Feeding Sites in Eastern Nebraska SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Stomoxys calcitrans; larval developmental sites; spatial variability ID SOIL ELECTRICAL-CONDUCTIVITY; STOMOXYS-CALCITRANS DIPTERA; FLIES DIPTERA; LARVAL DEVELOPMENT; TREATED TARGETS; CATTLE; DISPERSAL AB Residues at sites where stationary feeders were used to provide hay as supplemental forage for cattle during the winter are developmental substrates for immature stable flies, Stomoxys calcitrans (L.), in the central United States. Spatial patterns in physical (substrate depth, temperature, water content), chemical (pH, electrical conductivity [EClab], total nitrogen [N] and carbon [C], ammoniacal nitrogen [NH4-N], extractable phosphorus [P]), and biological (microbial respiration rate) substrate properties for two feeding sites were estimated and the correlations between these properties and adult emergence were characterized. Hay feeding sites had a circular footprint with residues extending approximate to 7m from the feeder. With the exception of extractable P and total N, all substrate properties exhibited spatial patterns centered on the feeder location. Adult stable fly emergence densities were significantly correlated with substrate microbial respiration rate, NH4-N concentration, EClab, total C concentration, pH, and moisture content. Logistic regression indicated that EC best predicted the probability of stable flies emerging from a substrate and that the other properties did not provide additional information. A better understanding of the physical, chemical, and biological conditions needed for stable fly larval development may help in identifying previously unrecognized developmental habitats and management of this pest. Targeted implementation of management practices such as sanitation and chemical treatments can be applied to smaller areas reducing labor and improving cost effectiveness. C1 [Wienhold, B. J.; Taylor, D. B.] ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. RP Wienhold, BJ (reprint author), ARS, USDA, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. EM brian.wienhold@ars.usda.gov RI Taylor, David/G-6025-2014 OI Taylor, David/0000-0002-4378-4867 NR 29 TC 5 Z9 5 U1 1 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 213 EP 221 DI 10.1603/EN11242 PG 9 WC Entomology SC Entomology GA 925SB UT WOS:000302780400002 PM 22506992 ER PT J AU Choate, B Drummond, FA AF Choate, Beth Drummond, Francis A. TI Ant Diversity and Distribution (Hymenoptera: Formicidae) Throughout Maine Lowbush Blueberry Fields in Hancock and Washington Counties SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Formicidae; lowbush blueberry; Maine; ant diversity ID COMMUNITY STRUCTURE; SPECIES RICHNESS; APHID PREDATOR; BIODIVERSITY; PESTICIDES; ASSEMBLAGE; ABUNDANCE; ULKEI; FAUNA; EDGE AB A 6-yr survey (2003-2008) identifying the ant fauna present in Maine lowbush blue-berry fields was conducted in Washington and Hancock Counties. Pitfall trapping, leaf litter, and hand collections, as well as protein and sugar baits were used to characterize the resident ant community in this habitat. Estimates of faunal richness as impacted by the blueberry crop stage (pruned or fruit-bearing), methods of pest management (grower standard, reduced-risk, or organic), and location within fields (middle, edge, or forested perimeters) were determined. In total, 42 species were collected from blueberry fields, comprising five subfamilies and 15 genera. Myrmica sculptilis Francoeur, Myrmica americana Weber, and Formica exsectoides Forel were the three most abundant species. Formica ulkei Emery, Myrmecina americana Emery, and Leptothorax canadensis Provancher represent new species records for Maine. Ants were most diverse in organic fields, and along the edge and within the wooded areas surrounding fields. Results suggest insecticide application reduces ant diversity. C1 [Choate, Beth] ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. [Drummond, Francis A.] Univ Maine, Sch Biol & Ecol, Orono, ME 04669 USA. RP Choate, B (reprint author), ARS, USDA, N Cent Agr Res Lab, Brookings, SD 57006 USA. EM beth.choate@ars.usda.gov FU Maine Agricultural and Forest Experiment Station; Maine blueberry industry; USDA/RAMP; USDA FX We thank Judith Collins for conducting collections from 2002 to 2005 and 2007. The staff of the University of Maine Blueberry Hill Farm and many undergraduate students aided in fieldwork (S. Ratten, K. Gould, L. Carpenter, D. Knarr, D. Thaxter, E. Davis, J. Curtis, and C. Brunton). The following blueberry companies and farms granted permission to conduct these studies on their lands: Jasper Wyman and Son, Milbridge, ME; Cherryfield Foods, Cherryfield, ME; Northeast Bluberry, Jonesboro, ME; Blueberry Hill Farm Experiment Station, Jonesboro, ME; Blue Barrens Farm, Columbia Falls, ME; Gramps Farm, Orland, ME; Hackmatack Farm, Penobscot, ME; and Spring River Farm, Cherryfield, ME. We also thank A. Calhoun for the use of private blueberry land. Many thanks to Andre Francouer for providing numerous hours sharing his knowledge of ant taxonomy and verifying species identification. Thank you to Andrei Alyokhin, William Glanz, Eleanor Groden, David Yarborough, Jonathan Lundgren, and Blair Sampson for reviewing a previous version of this manuscript. This research was supported by grants from the Maine Agricultural and Forest Experiment Station, the Maine blueberry industry, USDA/RAMP program and the USDA/Organic transitions program. NR 65 TC 6 Z9 7 U1 9 U2 33 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 222 EP 232 DI 10.1603/EN11096 PG 11 WC Entomology SC Entomology GA 925SB UT WOS:000302780400003 PM 22506993 ER PT J AU Duan, JJ Yurchenko, G Fuester, R AF Duan, Jian J. Yurchenko, Galina Fuester, Roger TI Occurrence of Emerald Ash Borer (Coleoptera: Buprestidae) and Biotic Factors Affecting Its Immature Stages in the Russian Far East SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Agrilus planipennis; indigenous; exotics and invasive species; parasitoids; wood borers ID AGRILUS-PLANIPENNIS COLEOPTERA; BEAUVERIA-BASSIANA; NATURAL ENEMIES; CHINA; HYMENOPTERA; PARASITOIDS; BRACONIDAE; EULOPHIDAE; ENCYRTIDAE; IMPACT AB Field surveys were conducted from 2008 to 2011 in the Khabarovsk and Vladivostok regions of Russia to investigate the occurrence of emerald ash borer, Agrilus planipennis Fairmaire, and mortality factors affecting its immature stages. We found emerald ash borer infesting both introduced North American green ash (Fraxinus pennsylvanica Marshall) and native oriental ashes (F. mandshurica Rupr. and F. rhynchophylla Hance) in both regions. Emerald ash borer densities (larvae/m(2) of phloem area) were markedly higher on green ash (11.3-76.7 in the Khabarovsk area and 77-245 in the Vladivostok area) than on artificially stressed Manchurian ash (2.2) or Oriental ash (10-59). Mortality of emerald ash borer larvae caused by different biotic factors (woodpecker predation, host plant resistance and/or undetermined diseases, and parasitism) varied with date, site, and ash species. In general, predation of emerald ash borer larvae by woodpeckers was low. While low rates (3-27%) of emerald ash borer larval mortality were caused by undetermined biotic factors on green ash between 2009 and 2011, higher rates (26-95%) of emerald ash borer larval mortality were caused by putative plant resistance in Oriental ash species in both regions. Little (<1%) parasitism of emerald ash borer larvae was observed in Khabarovsk; however, three hymenopteran parasitoids (Spathius sp., Atanycolus nigriventris Vojnovskaja-Krieger, and Tetrastichus planipennisi Yang) were observed attacking third - fourth instars of emerald ash borer in the Vladivostok area, parasitizing 0-8.3% of emerald ash borer larvae infesting Oriental ash trees and 7.3-62.7% of those on green ash trees (primarily by Spathius sp.) in two of the three study sites. Relevance of these findings to the classical biological control of emerald ash borer in newly invaded regions is discussed. C1 [Duan, Jian J.; Fuester, Roger] ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. [Yurchenko, Galina] Forest Management Agcy Russian Federat, Far E Forest Res Inst, Khabarovsk, Russia. RP Duan, JJ (reprint author), ARS, USDA, Beneficial Insects Intro Res Unit, Newark, DE 19713 USA. EM jian.duan@ars.usda.gov NR 31 TC 36 Z9 37 U1 3 U2 41 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 245 EP 254 DI 10.1603/EN11318 PG 10 WC Entomology SC Entomology GA 925SB UT WOS:000302780400006 PM 22506996 ER PT J AU White, WH Wilson, LT AF White, W. H. Wilson, L. T. TI Feasibility of Using an Alternative Larval Host and Host Plants to Establish Cotesia flavipes (Hymenoptera: Braconidae) in the Temperate Louisiana Sugarcane Ecosystem SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE conservation biological control; refuge; Poaceae; stemborer; energy sugarcane ID RELEASE STRATEGIES; BIOLOGICAL-CONTROL; BORER LEPIDOPTERA; MANAGEMENT; PYRALIDAE; CANE AB Attempts to establish Cotesia flavipes Cameron (Hymenoptera: Braconidae) in Louisiana sugarcane fields to control the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae) have been unsuccessful. Experiments were conducted to investigate the feasibility of using an alternative larval host and host plants to overcome barriers preventing establishment. In addition, we evaluated C. flavipes' ability to search for D. saccharalis in sugarcane without above-ground internodes. Diatraea evanescens Dyar (Lepidoptera: Crambidae) was investigated as an alternative host for C. flavipes. Cotesia flavipes was reared for five generations on D. evanescens without any indication of diminishing fitness as measured by days to parasite pupation and average cocoon mass weight. However, there was a significant reduction in percent parasitism, cocoon mass weight, and percent emergence when C. flavipes parasitized D. evanescens as compared with D. saccharalis, resulting in a 75% reduction in the gross reproductive rate (R-0). Greenhouse studies indicated little difference in parasitism of D. saccharalis on the weed hosts johnsongrass, Sorghum halepense (L.), and vaseygrass, Paspalum urvillei Steud. However, when planted as refuge plots, we found it difficult to establish infestations of D. saccharalis in either of these hosts, or in two energy sugarcanes. After 3 yr of infesting host plants and releasing parasitoids only one parasitized D. saccharalis larvae was recovered within the johnsongrass refuge. Diatraea evanescens readily established in vaseygrass; however, these larvae appear inaccessible to C. flavipes. In contrast, parasitism of D. saccharalis by C. flavipes infesting young sugarcane was 30%. C1 [White, W. H.] ARS, USDA, Sugarcane Res Unit, Houma, LA 70360 USA. [Wilson, L. T.] Texas A&M Univ, Agrilife Res & Extens Ctr, Beaumont, TX 77713 USA. RP White, WH (reprint author), ARS, USDA, Sugarcane Res Unit, Houma, LA 70360 USA. EM william.white@ars.usda.gov RI Wilson, Lloyd/E-9971-2011 FU American Sugar Cane League of the United States, Thibodaux, LA FX We thank Randy Richard for technical assistance in conducting greenhouse and field studies; Elta Duet for rearing insects used in these studies; and Jeannette Adams for fabricating isolation cages. These individuals are employees of the USDA, ARS Sugarcane Research Unit, Houma, LA. We gratefully acknowledge the financial support of the American Sugar Cane League of the United States, Thibodaux, LA. NR 31 TC 1 Z9 2 U1 3 U2 12 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 275 EP 281 DI 10.1603/EN11095 PG 7 WC Entomology SC Entomology GA 925SB UT WOS:000302780400009 PM 22506999 ER PT J AU Shapiro-Ilan, DI Gardner, WA Wells, L Wood, BW AF Shapiro-Ilan, David I. Gardner, Wayne A. Wells, Lenny Wood, Bruce W. TI Cumulative Impact of a Clover Cover Crop on the Persistence and Efficacy of Beauveria bassiana in Suppressing the Pecan Weevil (Coleoptera: Curculionidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Beauveria bassiana; biological control; cover crop; Curculio caryae; pecan ID ENTOMOPATHOGENIC FUNGI; METARHIZIUM-ANISOPLIAE; CARYAE COLEOPTERA; SOIL; ORCHARDS; SUSCEPTIBILITY; NEMATODES; ECOLOGY; CONIDIA; LARVAE AB The pecan weevil, Curculio caryae (Horn), is a key pest of pecan. Endemic levels of the entomopathogenic fungus Beauveria bassiana (Balsamo) Vuillemin can occur in pecan orchards and contribute to natural control of C. caryae. Commercial formulations of the fungus can also be applied for suppression of C. caryae. We hypothesized that a clover cover crop enhances B. bassiana efficacy and persistence (e.g., by protecting the fungus against abiotic environmental stresses). The hypothesis was tested by conducting field trials in a pecan orchard in Byron, GA, in 2009 and 2010. The study included four treatments arranged in a factorial with two levels of fungus (endemic fungus only, and application of a commercial B. bassiana product), and two levels of clover (white clover, Trifolium repens L., and no clover). Fungal persistence was measured by determining the number of CFUs per gram of soil over time (during 42 d postapplication of B. bassiana in 2009 and 29 d in 2010). Efficacy was measured by capturing naturally emerging C. caryae and subsequently determining mortality and mycosis (over 24 d in 2009 and 17 d in 2010). In 2009, greater prevalence of B. bassiana conidia was detected in plots receiving fungal applications compared with no fungus applications, and no clear effect of clover was observed in plots receiving B. bassiana applications in either year. In 2010, B. bassiana prevalence in the endemic fungus plus clover treatment was higher than fungus without clover, and was similar to plots receiving additional B. bassiana applications. Given that we observed enhanced persistence of endemic B. bassiana in 2010 but not 2009, the impact of clover appears to be a cumulative effect. Mortality of C. caryae (averaged over the sampling periods) ranged between 68-74% in plots receiving B. bassiana applications and 51-56% in plots with endemic fungus only. C. caryae mortality and mycosis data also provided evidence that endemic B. bassiana efficacy was enhanced by clover relative to plots without clover (with no clear clover effect on plots receiving fungus applications). Thus, we conclude that natural control of C. caryae can increase when clover is grown in pecan orchards with endemic populations of B. bassiana. C1 [Shapiro-Ilan, David I.; Wood, Bruce W.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Gardner, Wayne A.] Univ Georgia, Dept Entomol, Coll Agr & Environm Sci, Griffin, GA 30223 USA. [Wells, Lenny] Univ Georgia, Dept Hort, Tifton, GA 31793 USA. RP Shapiro-Ilan, DI (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. EM david.shapiro@ars.usda.gov FU Georgia Agricultural Commodity Commission for Pecans FX We thank Terri Brearley, Leigh Ann Colley, Wanda Evans, Leighann Fall, Kathy Halat, and Hal Peeler for technical assistance. We thank the Georgia Agricultural Commodity Commission for Pecans for funding a portion of this research. NR 47 TC 4 Z9 4 U1 4 U2 14 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 298 EP 307 DI 10.1603/EN11229 PG 10 WC Entomology SC Entomology GA 925SB UT WOS:000302780400012 PM 22507002 ER PT J AU Boughton, AJ Pemberton, RW AF Boughton, Anthony J. Pemberton, Robert W. TI Biology and Reproductive Parameters of the Brown Lygodium Moth, Neomusotima conspurcatalis - A New Biological Control Agent of Old World Climbing Fern in Florida SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Lygodium microphyllum; mating biology; sexual selection; weed biocontrol ID INVASIVE WEED; LEPIDOPTERA CRAMBIDAE; AUSTROMUSOTIMA-CAMPTOZONALE; MICROPHYLLUM SCHIZAEACEAE; LYGODIACEAE; ESTABLISHMENT; AUSTRALIA; PREDATION; ASIA AB Neomusotima conspurcatalis Warren was first released in Florida as a weed biological control agent against Old World climbing fern in 2008, and readily established large field populations. A related biocontrol agent, Austromusotima camptozonale, had previously failed to establish despite several years of releases. Life history studies were conducted to determine whether aspects of the reproductive biology of N. conspurcatalis might account for these different outcomes. At 26.5 degrees C, development from egg to adult averaged 22.2 +/- 0.1 d, with 75% of larvae emerging as adults. The sex ratio averaged 1:0.8 (male:female), with both sexes emerging at the same time. Female moths typically mated once, on the first night after emergence, and began oviposition the next night. Females laid half their eggs on the first night and lived an average of 10.7 +/- 0.8 d. Individual females maintained in cages with a male-biased sex ratio (3 male:1 female) produced significantly more larvae over their lifetime (140 +/- 6.6 larvae) than individual females maintained at a ratio of 1 male:1 female (111 +/- 9.1 larvae). Sexual selection, either through 'male-male competition' or 'female choice' was likely responsible for this result, because there were no significant differences in mating frequency, duration of ovipositional period or female longevity to otherwise explain the difference. Two-fold greater lifetime reproductive output (average 127 +/- 6.3 larvae) and deposition of half this output on the first night of oviposition, likely contributed to rapid field establishment of N. conspurcatalis compared with A. camptozonale. C1 [Boughton, Anthony J.; Pemberton, Robert W.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. RP Boughton, AJ (reprint author), ARS, USDA, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM anthonyboughton@yahoo.com FU Florida Fish & Wildlife Conservation Commission; South Florida Water Management District FX We thank Anthony Wright (retired), Jeff Makinson, and Ryan Zonneveld (Commonwealth Scientific and Industrial Research Organization Entomology) for making field collections of N. conspurcatalis. Chris Bennett (University of Florida, [retired]) conducted the rearing and egg viability studies. Rachel Taylor (USDA-ARS) maintained laboratory colonies and assisted with the reproductive studies. Luke Kasarjian (USDA-ARS) made weekly collections of L. microphyllum foliage used for colony maintenance. This research was funded by financial support from the Florida Fish & Wildlife Conservation Commission and the South Florida Water Management District. NR 28 TC 8 Z9 8 U1 2 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 308 EP 316 DI 10.1603/EN11146 PG 9 WC Entomology SC Entomology GA 925SB UT WOS:000302780400013 PM 22507003 ER PT J AU Cooper, WR Spurgeon, DW AF Cooper, W. Rodney Spurgeon, Dale W. TI Injury to Cotton by Adult Lygus hesperus (Hemiptera: Miridae) of Different Gender and Reproductive States SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE western tarnished plant bug; cotton; Gossypium; within-plant distribution; Lygus ID OVIPOSITION BEHAVIOR; HETEROPTERA; KNIGHT; GROWTH; PLANT AB Lygus hesperus Knight (Hemiptera: Miridae) is a key pest of cotton (Gossypium spp.) in the western United States that injures floral buds (squares) and developing fruit (bolls). However, no clear relationship between Lygus population level and plant injury has been established. Age-dependent feeding activity by L. hesperus is a possible source of variation that has not been examined for its influence in studies of the impact of Lygus on cotton. Recent video-based laboratory studies indicated that feeding behaviors and trivial movement varied among L. hesperus adults of different gender and reproductive states (prereproductive; reproductive and unmated; and reproductive and mated). We compared within-plant distributions and accumulations of feeding injury to intact cotton plants corresponding to adult L. hesperus of different gender and reproductive states. Adult females, regardless of reproductive state, were observed on squares and axillary buds more often than were males. Additionally, prereproductive adults were observed on squares and axillary buds more often than were mated or unmated reproductive adults, regardless of gender. Plants that were exposed to prereproductive adults exhibited more abscised squares and more squares with injured anthers compared with plants exposed to reproductive adults. However, feeding injury did not differ by insect mating status or gender. These results are consistent with results of our previous video-based assays, and indicate adult reproductive state represents a source of variation that should be controlled in studies to evaluate Lygus-induced injury to cotton and other crop plants. C1 [Cooper, W. Rodney; Spurgeon, Dale W.] ARS, USDA, Shafter Cotton Res Stn, Shafter, CA 93263 USA. RP Cooper, WR (reprint author), ARS, USDA, Shafter Cotton Res Stn, 17053 N Shafter Ave, Shafter, CA 93263 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 FU Cotton Inc. [11-852CA] FX We thank Stephen Wingard for technical assistance in conducting these assays. This research was supported in part by Cotton Inc. (grant 11-852CA). NR 13 TC 6 Z9 6 U1 0 U2 8 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 342 EP 348 DI 10.1603/EN11236 PG 7 WC Entomology SC Entomology GA 925SB UT WOS:000302780400017 PM 22507007 ER PT J AU Knight, A Light, D Chebny, V AF Knight, Alan Light, Douglas Chebny, Vincent TI Evaluating Dispensers Loaded With Codlemone and Pear Ester for Disruption of Codling Moth (Lepidoptera: Tortricidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE apple; Cydia pomonella; sex pheromone; monitoring; mating disruption ID CYDIA-POMONELLA; MATING DISRUPTION; SEX-PHEROMONE; APPLE ORCHARDS; BLENDS AB Polyvinyl chloride polymer (PVC) dispensers loaded with ethyl (E, Z)-2,4-decadienoate (pear ester) plus the sex pheromone, (E, E)-8,10-dodecadien-1-ol (codlemone) of codling moth, Cydia pomonella (L.), were compared with PVC dispensers and a commercial dispenser (Isomate-C Plus) loaded with codlemone. Evaluations were conducted in replicated plots (0.1 0.2 ha) in apple, Malus domestica (Borkhausen) during both generations of codling moth from 2007 to 2009. Dispensers were applied at 1,000 ha(-1). Male captures in traps baited with virgin female moths and codlemone lures were recorded. Residual analysis of field-aged dispensers over both moth generations was conducted. Dispensers exhibited linear declines in release rates of both attractants, and pear ester was released at a significantly higher rate than codlemone during both time periods. The proportion of virgin female-baited traps catching males was significantly lower with combo dispenser TRE24 (45/110, mg codlemone/mg pear ester) during the second generation in 2007 and the combo dispensers TRE144 (45/75) and TRE145 (75/45) during the first generation in 2008 compared with Isomate-C Plus. Similarly, male catches in female-baited traps in plots treated with the combo dispensers TRE144 during the first generation in 2008 and TRE23 (75/110) during the second generation, in 2007 were significantly lower than in plots treated with Isomate-C Plus. No significant differences were found for male catches in codlemone-baited traps in plots treated with Isomate-C Plus and any of the combo dispensers. However, male catches were significantly lower in plots treated with Cidetrak CM (codlemone-only dispenser) than the combo TRE144 dispenser during both generations in 2009. C1 [Knight, Alan] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Light, Douglas] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Chebny, Vincent] Trece Inc, Adair, OK 74330 USA. RP Knight, A (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov NR 31 TC 5 Z9 5 U1 1 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 399 EP 406 DI 10.1603/EN11309 PG 8 WC Entomology SC Entomology GA 925SB UT WOS:000302780400025 PM 22507015 ER PT J AU Knight, AL Light, DM AF Knight, Alan L. Light, Douglas M. TI Monitoring Codling Moth (Lepidoptera: Tortricidae) in Sex Pheromone-Treated Orchards With (E)-4,8-Dimethyl-1,3,7-Nonatriene or Pear Ester in Combination With Codlemone and Acetic Acid SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE apple; Cydia pomonella; traps; mating disruption; plant volatiles ID HOST-PLANT VOLATILES; CYDIA-POMONELLA; BAITED TRAPS; ETHYL (E; KAIROMONE; APPLE; CATCH; Z)-2,4-DECADIENOATE; BLENDS AB Traps baited with ethyl (E,Z)-2,4-decadienoate (pear ester) or (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) in two-or three-way combinations with the sex pheromone (E, E)-8,10-dodecadien-1-ol (codlemone) and acetic acid (AA) were evaluated for codling moth, Cydia pomonella (L.). All studies were conducted in apple orchards, Malus domestica Borkhausen, treated with sex pheromone dispensers during 2010. Septa were loaded with codlemone, DMNT, and pear ester individually or codlemone with either DMNT or pear ester together (combo lures). Polyethylene vials loaded with AA were added as a co-lure. Residual analyses of field-aged combo lures and weight loss of the AA co-lure were conducted. AA vials lost 50-150 mg wk(-1). Weekly weight loss was not affected by field aging, but was closely correlated with the daily mean temperature. Pear ester was released from septa at a slightly higher but nonsignificant rate than codlemone. DMNT was released at a significantly higher rate than codlemone, and lures were effective for 4 wk. The addition of codlemone to traps baited with either host plant volatile plus AA had either no effect or significantly increased total moth catches. The addition of AA significantly increased the catch of female moths with either combo lure. Total moth catches in traps baited with pear ester or DMNT combo lures and AA did not differ and were either significantly higher or similar to the pear ester combo lure. These data suggest that codling moth may be more effectively monitored in sex pheromone-treated orchards with multi-component lures, including codlemone, AA, and host plant volatiles. C1 [Knight, Alan L.] ARS, USDA, Wapato, WA 98951 USA. [Light, Douglas M.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Knight, AL (reprint author), ARS, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM alan.knight@ars.usda.gov NR 35 TC 9 Z9 9 U1 3 U2 23 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 407 EP 414 DI 10.1603/EN11310 PG 8 WC Entomology SC Entomology GA 925SB UT WOS:000302780400026 PM 22507016 ER PT J AU Spurgeon, DW AF Spurgeon, D. W. TI Physiological Consequences of Laboratory Rearing of Lygus hesperus (Hemiptera: Miridae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE rearing; plant bug; diapause; development time ID KNIGHT; DIAPAUSE AB Several aspects of the basic biology of the western tarnished plant bug, Lygus hesperus Knight, are poorly known despite the economic importance of this species. Among these are the factors regulating the adult diapause. Reports of recent studies questioned the validity of earlier reports of diapause in L. hesperus, in part because of the demonstrated loss of diapause response in insects obtained from long-standing laboratory colonies. However, use of laboratory reared insects would facilitate additional diapause research, so long as those insects exhibit a diapause response similar to that of the field population. L. hesperus, originating as eggs from field-collected insects, were reared in the laboratory for four generations to examine corresponding changes in selected biological characteristics. Over the course of the four generations, incidence of diapause in both L. hesperus genders decreased whereas the frequency of oviposition by virgin females increased. Measurable changes were not observed in frequency of occurrence of a specific fat body type (glass bead fat) or nymphal development time. These results suggest L. hesperus used in diapause research should be as close to the field population as possible, but no further removed than three generations. Results further demonstrate variability among different biological characteristics in their responses to selection from laboratory rearing. Collectively, these findings demonstrate the importance of understanding the influences of rearing on specific biological characteristics under study, and the need to verify the similarity of laboratory-reared insects to their native counterparts in studies used to draw inferences regarding the field population. C1 USDA ARS, WICSRU, Shafter Cotton Res Stn, Shafter, CA 93263 USA. RP Spurgeon, DW (reprint author), USDA ARS, WICSRU, Shafter Cotton Res Stn, 17053 N Shafter Ave, Shafter, CA 93263 USA. EM dale.spurgeon@ars.usda.gov NR 11 TC 9 Z9 9 U1 2 U2 10 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2012 VL 41 IS 2 BP 415 EP 419 DI 10.1603/EN11248 PG 5 WC Entomology SC Entomology GA 925SB UT WOS:000302780400027 PM 22507017 ER PT J AU Chendev, YG Burras, CL Sauer, TJ AF Chendev, Yu. G. Burras, C. L. Sauer, T. J. TI Transformation of forest soils in Iowa (United States) under the impact of long-term agricultural development SO EURASIAN SOIL SCIENCE LA English DT Article AB The evolution of automorphic cultivated soils of the Fayette series (the order of Alfisols)-close analogues of gray forest soils in the European part of Russia-was studied by the method of agrosoil chronosequences in the lower reaches of the Iowa River. It was found that the old-arable soils are characterized by an increase in the thickness of humus horizons and better aggregation; they are subjected to active biogenic turbation by rodents; some alkalization of the soil reaction and an increase in the sum of exchangeable bases also take place. These features are developed against the background of active eluvial-illuvial differentiation and gleyzation of the soil profiles under conditions of a relatively wet climate typical of the ecotone between the zones of prairies and broadleaved forests in the northeast Central Plains of the United States. C1 [Chendev, Yu. G.] Belgorod State Univ, Belgorod 308015, Russia. [Burras, C. L.] Iowa State Univ, Ames, IA 50010 USA. [Sauer, T. J.] USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Chendev, YG (reprint author), Belgorod State Univ, Ul Pobedy 85, Belgorod 308015, Russia. FU Fulbright Program FX The authors are grateful to District Forester of the Iowa Department of Natural Resources Mark Vitosh for his valuable help in the selection of cartographic materials used in this study and for the geobotanic description of the key site. We are also thankful to staff members of the USDA National Soil Tilth Laboratory Kevin Jensen and Kent Heikens for the logistic support of field works. This study was supported by the Fulbright Program. NR 27 TC 3 Z9 3 U1 0 U2 2 PU MAIK NAUKA/INTERPERIODICA/SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013-1578 USA SN 1064-2293 J9 EURASIAN SOIL SCI+ JI Eurasian Soil Sci. PD APR PY 2012 VL 45 IS 4 BP 357 EP 367 DI 10.1134/S1064229312040035 PG 11 WC Soil Science SC Agriculture GA 926DG UT WOS:000302810900003 ER EF