FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Espindola, AS Garzon, CD Fletcher, J Schneider, WL AF Espindola, A. S. Garzon, C. D. Fletcher, J. Schneider, W. L. TI Validation of EDNA, a newly developed bioinformatics tool, for detection of Phakopsora pachyrhizi from metagenomic samples SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Espindola, A. S.; Garzon, C. D.; Fletcher, J.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W. L.] USDA, Ft Detrick, MD USA. RI Espindola, Andres/A-5056-2016 OI Espindola, Andres/0000-0002-9658-0673 NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 35 EP 35 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800184 ER PT J AU Espindola, AS Garzon, CD Fletcher, J Schneider, WL AF Espindola, A. S. Garzon, C. D. Fletcher, J. Schneider, W. L. TI Validation of EDNA, a newly developed bioinformatics tool, for the detection of Pythium ultimum from metagenomic samples SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Espindola, A. S.; Garzon, C. D.; Fletcher, J.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W. L.] USDA ARS, Ft Detrick, MD USA. RI Espindola, Andres/A-5056-2016 OI Espindola, Andres/0000-0002-9658-0673 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 35 EP 35 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800185 ER PT J AU Fountain, J Raruang, Y Luo, M Brown, RL Chen, Z AF Fountain, J. Raruang, Y. Luo, M. Brown, R. L. Chen, Z. TI Identification of maize WRKY transcription factors responding to Aspergillus flavus infection and their roles in resistance to aflatoxin contamination SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Fountain, J.; Raruang, Y.; Luo, M.; Chen, Z.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Brown, R. L.] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 40 EP 41 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800211 ER PT J AU French, R Seifers, D Wegulo, SN Tatineni, S AF French, R. Seifers, D. Wegulo, S. N. Tatineni, S. TI Triticum mosaic virus: Genetic evidence for recent population expansion and balancing selection SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [French, R.; Tatineni, S.] ARS, USDA, Lincoln, NE USA. [Seifers, D.] Kansas State Univ, Hays, KS USA. [Wegulo, S. N.] Univ Nebraska, Lincoln, NE USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 42 EP 42 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800219 ER PT J AU Friskop, A Gulya, T Acevedo, M Harveson, R Humann, R Markell, S AF Friskop, A. Gulya, T. Acevedo, M. Harveson, R. Humann, R. Markell, S. TI Race diversity of Puccinia helianthi (sunflower rust) in the Northern Great Plains in 2011 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Friskop, A.; Acevedo, M.; Humann, R.; Markell, S.] N Dakota State Univ, Fargo, ND 58105 USA. [Gulya, T.] ARS, USDA, Sunflower Res Unit, Fargo, ND USA. [Harveson, R.] Univ Nebraska, Scottsbluff, NE USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 42 EP 42 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800220 ER PT J AU Garrido, PA Garzon, CD Grunwald, NJ Weiland, J AF Garrido, P. A. Garzon, C. D. Gruenwald, N. J. Weiland, J. TI Population structure and genetic diversity of three species of Pythium isolated from forest tree nursery soils in Oregon and Washington SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Garrido, P. A.; Garzon, C. D.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Gruenwald, N. J.; Weiland, J.] ARS, USDA, Corvallis, OR USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 43 EP 43 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800225 ER PT J AU Gaskins, VL Vico, I Peter, KA Janisiewicz, WJ Jurick, WM AF Gaskins, V. L. Vico, I. Peter, K. A. Janisiewicz, W. J. Jurick, W. M. TI Sensitivity of Penicillium spp. from decayed apple fruit to postharvest fungicides and identification of a new codon associated with thiabendazole resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Gaskins, V. L.; Vico, I.; Peter, K. A.] ARS, USDA, Beltsville, MD USA. [Janisiewicz, W. J.] ARS, USDA, AFRS, Kearneysville, WV USA. [Jurick, W. M.] ARS, USDA, Food Qual Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 43 EP 43 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800227 ER PT J AU Gaskins, VL Peter, KA Vico, I Janisiewicz, WJ Jurick, WM AF Gaskins, V. L. Peter, K. A. Vico, I. Janisiewicz, W. J. Jurick, W. M. TI Rapid and specific detection of Pencillium species causing blue mold decay on pome fruit in storage using molecular tools SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Gaskins, V. L.; Peter, K. A.; Vico, I.] ARS, USDA, Beltsville, MD USA. [Janisiewicz, W. J.] ARS, USDA, AFRS, Kearneysville, WV USA. [Jurick, W. M.] ARS, USDA, Food Qual Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 43 EP 43 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800226 ER PT J AU Grunwald, NJ AF Grunwald, N. J. TI How do Phytophthora pathogens emerge? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Grunwald, N. J.] ARS, USDA, Corvallis, OR USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 48 EP 48 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800251 ER PT J AU Guan, W Shao, J Singh, R Davis, R Zhao, T Huang, Q AF Guan, W. Shao, J. Singh, R. Davis, R. Zhao, T. Huang, Q. TI A TaqMan-based real-time PCR assay for specific detection and quantification of Xylella fastidiosa strains causing bacterial leaf scorch in oleander SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Guan, W.; Huang, Q.] ARS, FNPRU, US Natl Arboretum, USDA, Beltsville, MD USA. [Shao, J.; Davis, R.] ARS, MPPL, PSI, USDA, Beltsville, MD USA. [Singh, R.] Louisiana State Univ, AgCtr, Plant Dis Diagnost Clin, Baton Rouge, LA 70803 USA. [Zhao, T.] Chinese Acad Agr Sci, Beijing 100193, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 48 EP 48 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800253 ER PT J AU Hammond, J Henderson, DC Bagewadi, B Fischer, KF Wang, D Melcher, U Perry, KL Jordan, RL Fauquet, CM AF Hammond, J. Henderson, D. C. Bagewadi, B. Fischer, K. F. Wang, D. Melcher, U. Perry, K. L. Jordan, R. L. Fauquet, C. M. TI Progress in development of a Universal Plant Virus Microarray for the detection and identification of plant viruses SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Hammond, J.; Henderson, D. C.; Jordan, R. L.] ARS, USDA, FNPRU, Beltsville, MD USA. [Bagewadi, B.; Fauquet, C. M.] Danforth Plant Sci Ctr, St Louis, MO USA. [Fischer, K. F.] Univ Utah, Salt Lake City, UT USA. [Wang, D.] Washington Univ, St Louis, MO USA. [Melcher, U.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Perry, K. L.] Cornell Univ, Ithaca, NY USA. NR 0 TC 1 Z9 1 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 49 EP 49 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800258 ER PT J AU Hanson, LE Mo, T Goodwill, TR AF Hanson, L. E. Mo, T. Goodwill, T. R. TI Variability in Phoma species affecting sugar beet SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Hanson, L. E.; Goodwill, T. R.] ARS, USDA, E Lansing, MI USA. [Mo, T.] Michigan State Univ, E Lansing, MI 48824 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 50 EP 50 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800261 ER PT J AU Harveson, R Gulya, T Karasev, A Lenardon, S AF Harveson, R. Gulya, T. Karasev, A. Lenardon, S. TI The appearance of an unknown viruslike disease of sunflowers in Nebraska SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Harveson, R.] Univ Nebraska, Scottsbluff, NE USA. [Gulya, T.] USDA, Fargo, ND USA. [Karasev, A.] Univ Idaho, Moscow, ID 83843 USA. [Lenardon, S.] CIAP INTA, Cordova, Argentina. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 51 EP 51 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800268 ER PT J AU Haudenshield, JS Bowen, CR Hartman, GL AF Haudenshield, J. S. Bowen, C. R. Hartman, G. L. TI Pentaplex Q-PCR quantifies DNA from fungi causing anthracnose, brown stem rot, and charcoal rot in field samples of soybean SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Haudenshield, J. S.; Bowen, C. R.; Hartman, G. L.] ARS, USDA, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 51 EP 51 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800269 ER PT J AU Nopsa, JH Pfender, WF AF Nopsa, J. Hernandez Pfender, W. F. TI Effect of temperature on latent period of wheat stem rust (Puccinia graminis subsp graminis f. sp tritici) isolates across different wheat cultivars SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Nopsa, J. Hernandez; Pfender, W. F.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 52 EP 52 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800276 ER PT J AU Hodges, AC Ratcliffe, ST Draper, MA Stocks, SD AF Hodges, A. C. Ratcliffe, S. T. Draper, M. A. Stocks, S. D. TI Protect US-Engaging researchers, county faculty, and K-12 teachers in invasive species education SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Hodges, A. C.; Stocks, S. D.] Univ Florida, Gainesville, FL USA. [Ratcliffe, S. T.] Univ Illinois, Urbana, IL 61801 USA. [Draper, M. A.] USDA, NIFA, Washington, DC 20250 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 54 EP 54 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800285 ER PT J AU Jacobs, J Woeste, K Ostry, M Michler, C AF Jacobs, J. Woeste, K. Ostry, M. Michler, C. TI Inhibition of Ophiognomonia clavigignenti-juglandacearum in vitro by fungi associated with butternut, Japanese walnut, and hybrid butternut SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Jacobs, J.] Purdue Univ, Dept Forestry & Nat Resources, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Woeste, K.; Michler, C.] Purdue Univ, Dept Forestry & Nat Resources, USDA, US Forest Serv,Hardwood Tree Improvement & Regene, W Lafayette, IN 47907 USA. [Ostry, M.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 57 EP 57 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800302 ER PT J AU Jaime, R Foley, M Antilla, L Cotty, PJ AF Jaime, R. Foley, M. Antilla, L. Cotty, P. J. TI Long-term and area-wide influences of atoxigenic strain biocontrol technology for aflatoxin contamination SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Jaime, R.] Univ Arizona, Tucson, AZ USA. [Foley, M.; Antilla, L.] Arizona Cotton Res & Protect Council, Phoenix, AZ USA. [Cotty, P. J.] Univ Arizona, USDA, ARS, Tucson, AZ USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 58 EP 58 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800303 ER PT J AU Jang, C Nam, J Li, M Kim, Y Yu, S Kim, H Beom, J Hammond, J Lim, H AF Jang, C. Nam, J. Li, M. Kim, Y. Yu, S. Kim, H. Beom, J. Hammond, J. Lim, H. TI Interaction of Nicotiana benthamiana PSBO1 with AltMV TGB3 correlates with chloroplast vesiculation and veinal necrosis caused by TGB3 overexpression SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Jang, C.; Nam, J.; Li, M.; Kim, Y.; Yu, S.; Kim, H.; Lim, H.] Chungnam Natl Univ, Taejon, South Korea. [Beom, J.] Chungcheongnam Do Agr Res & Extens Serv, Chrysanthemum Expt Stn, Yesan, South Korea. [Hammond, J.] ARS, USDA, FNPRU, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 58 EP 58 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800306 ER PT J AU Janisiewicz, WJ Jurick, WM Vico, I Peter, KA Buyer, JS AF Janisiewicz, W. J. Jurick, W. M. Vico, I. Peter, K. A. Buyer, J. S. TI Resident bacteria of plums and their potential for controlling brown rot after harvest SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Janisiewicz, W. J.] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV USA. [Jurick, W. M.; Vico, I.; Peter, K. A.] ARS, USDA, Food Qual Lab, Beltsville, MD USA. [Buyer, J. S.] ARS, USDA, Sustainable Agr Syst Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 58 EP 58 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800307 ER PT J AU Jia, Y Zhang, Z Xing, J Wang, Y Correll, J Cartwright, R AF Jia, Y. Zhang, Z. Xing, J. Wang, Y. Correll, J. Cartwright, R. TI Natural variation and evolution of the avirulence genes in Magnaporthe oryzae SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Jia, Y.] ARS, USDA, DBNRRC, Stuttgart, AR USA. [Zhang, Z.; Wang, Y.] Zhejiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China. [Xing, J.] Natl Hybrid Rice Res & Dev Ctr, Changsha, Hunan, Peoples R China. [Correll, J.; Cartwright, R.] Univ Arkansas, Fayetteville, AR 72701 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 59 EP 59 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800309 ER PT J AU Johnson-Brousseau, SA Kosta, KL Copeland, G Henkes, M Bulluck, R Ghosh, S AF Johnson-Brousseau, S. A. Kosta, K. L. Copeland, G. Henkes, M. Bulluck, R. Ghosh, S. TI Steam disinfection of Phytophthora ramorum from research field soils at the National Ornamentals Research Site at Dominican University of California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Johnson-Brousseau, S. A.; Copeland, G.; Henkes, M.; Ghosh, S.] Dominican Univ Calif, San Rafael, CA USA. [Kosta, K. L.] Calif Dept Food & Agr, Sacramento, CA 95814 USA. [Bulluck, R.] USDA, APHIS, PPQ, CPHST, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 59 EP 60 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800312 ER PT J AU Johnson-Brousseau, SA Kosta, KL Hernandez, J Rooney-Latham, S Arias, R Pelican, T Widmer, TL Bulluck, R Ghosh, S AF Johnson-Brousseau, S. A. Kosta, K. L. Hernandez, J. Rooney-Latham, S. Arias, R. Pelican, T. Widmer, T. L. Bulluck, R. Ghosh, S. TI Steam disinfection of Phytophthora ramorum from field soil in a US federally quarantined nursery SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Johnson-Brousseau, S. A.; Ghosh, S.] Dominican Univ Calif, San Rafael, CA USA. [Kosta, K. L.; Rooney-Latham, S.] Calif Dept Food & Agr, Sacramento, CA 95814 USA. [Hernandez, J.] Morris Wholesale Nursery, Riverbank, CA USA. [Arias, R.] Calif Dept Food & Agr, Riverside, CA USA. [Pelican, T.] Stanislaus Cty Agr Commissioners Off, Modesto, CA USA. [Widmer, T. L.] ARS, USDA, FDWSRU, Ft Detrick, MD USA. [Bulluck, R.] USDA, APHIS, PPQ, CPHST, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 60 EP 60 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800313 ER PT J AU Kalia, B Wilson, DL Bowden, RL Gill, BS AF Kalia, B. Wilson, D. L. Bowden, R. L. Gill, B. S. TI Evaluation of Aegilops tauschii as a source of adult plant resistance to leaf rust of wheat SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kalia, B.; Wilson, D. L.; Gill, B. S.] Kansas State Univ, Manhattan, KS 66506 USA. [Bowden, R. L.] ARS, USDA, Manhattan, KS USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 61 EP 61 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800318 ER PT J AU Keremane, M Ramadugu, C Stover, E Halbert, SE Duan, Y Lee, RF AF Keremane, M. Ramadugu, C. Stover, E. Halbert, S. E. Duan, Y. Lee, R. F. TI Screening citrus and its relatives in Aurantioideae for tolerance to huanglongbing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Keremane, M.] ARS, USDA, Riverside, CA USA. [Ramadugu, C.] Univ Calif Riverside, Riverside, CA 92521 USA. [Stover, E.; Duan, Y.] ARS, USDA, USHRL, Ft Pierce, FL USA. [Halbert, S. E.] FDACS Div Plant Ind, Gainesville, FL USA. [Lee, R. F.] ARS, Natl Clonal Germplasm Repository Citrus & Dates, USDA, Riverside, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 63 EP 63 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800328 ER PT J AU Kim, M Stewart, JE Ota, Y Hanna, JW Ross-Davis, AL Klopfenstein, NB AF Kim, M. Stewart, J. E. Ota, Y. Hanna, J. W. Ross-Davis, A. L. Klopfenstein, N. B. TI Phylogenetic relationships among northern hemisphere Armillaria species based on the tef-1 alpha locus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kim, M.] Kookmin Univ, Seoul, South Korea. [Stewart, J. E.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. [Ota, Y.] Forestry & Forest Prod Res Inst, Tsukuba, Ibaraki 305, Japan. [Hanna, J. W.; Ross-Davis, A. L.; Klopfenstein, N. B.] USDA, FS, Rocky Mt Res Stn, Moscow, ID USA. NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 63 EP 64 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800333 ER PT J AU Kim, W Chen, W AF Kim, W. Chen, W. TI Production of phytotoxin solanapyrones and generation of solanapyrone-deficient mutants in Ascochyta rabiei SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kim, W.] Washington State Univ, Pullman, WA 99164 USA. [Chen, W.] ARS, USDA, Pullman, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 64 EP 64 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800334 ER PT J AU Kornev, K Ignatov, A Karlov, A Karlov, G Dzhalilov, F Pekhtereva, E Luster, D AF Kornev, K. Ignatov, A. Karlov, A. Karlov, G. Dzhalilov, F. Pekhtereva, E. Luster, D. TI Dickeya spp.-Emerging pathogen of potato in Russia SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kornev, K.; Ignatov, A.; Pekhtereva, E.] Russian Res Inst Phytopathol, Moscow, Russia. [Karlov, A.; Karlov, G.; Dzhalilov, F.] Russian State Agrarian Univ MSKhA, Moscow, Russia. [Luster, D.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA. RI Ignatov, Alexander/L-3438-2013 OI Ignatov, Alexander/0000-0003-2948-753X NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 64 EP 65 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800339 ER PT J AU Kousik, CS Adkins, S Webster, CG Turechek, W Roberts, PD AF Kousik, C. S. Adkins, S. Webster, C. G. Turechek, W. Roberts, P. D. TI Effect of SqVYV-resistant pollenizers on development and spread of watermelon vine decline in seedless watermelon SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kousik, C. S.] ARS, USDA, Charleston, SC USA. [Adkins, S.; Webster, C. G.; Turechek, W.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. [Roberts, P. D.] Univ Florida, Immokalee, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 65 EP 65 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800341 ER PT J AU Kriss, AB Gottwald, TR Paul, PA Madden, LV AF Kriss, A. B. Gottwald, T. R. Paul, P. A. Madden, L. V. TI Wavelet analysis as a statistical tool for spatial and temporal analysis of epidemics SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kriss, A. B.] ARS, USDA, Ft Pierce, FL USA. [Gottwald, T. R.] USDA, Ft Pierce, FL USA. [Paul, P. A.; Madden, L. V.] Ohio State Univ, Wooster, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 65 EP 65 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800344 ER PT J AU Kriss, AB Paul, PA Madden, LV AF Kriss, A. B. Paul, P. A. Madden, L. V. TI Climate patterns as predictors of Stagonospora nodorum glume blotch in Ohio SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kriss, A. B.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL USA. [Paul, P. A.; Madden, L. V.] Ohio State Univ, Wooster, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 65 EP 66 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800345 ER PT J AU Kunta, M Setamou, M Skaria, M Rascoe, JE Li, W Nakhla, MK da Graca, JV AF Kunta, M. Setamou, M. Skaria, M. Rascoe, J. E. Li, W. Nakhla, M. K. da Graca, J. V. TI First report of citrus huanglongbing in Texas SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kunta, M.] Texas A&M Univ Kingsville, Weslaco, TX USA. [Setamou, M.; Skaria, M.; da Graca, J. V.] Texas A&M Univ, Kingsville Citrus Ctr, Weslaco, TX USA. [Rascoe, J. E.; Li, W.; Nakhla, M. K.] USDA, APHIS, Beltsville, MD 20705 USA. NR 0 TC 9 Z9 9 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 66 EP 66 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800347 ER PT J AU Lakshman, DK Chauhan, K AF Lakshman, D. K. Chauhan, K. TI Biorational alternatives to control the soilborne plant pathogen Rhizoctonia solani SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lakshman, D. K.] ARS, USDA, FNPRU, Beltsville, MD USA. [Lakshman, D. K.] SASL, Beltsville, MD USA. [Chauhan, K.] ARS, USDA, IIBBL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 66 EP 66 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800349 ER PT J AU Larkin, RP Halloran, JM AF Larkin, R. P. Halloran, J. M. TI Longer-term potato cropping system effects on soilborne diseases and tuber yield SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Larkin, R. P.; Halloran, J. M.] ARS, USDA, New England Plant Soil & Water Lab, Orono, ME USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 67 EP 67 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800352 ER PT J AU Lartey, RT Evans, RG Caesar-TonThat, T Caesar, AJ AF Lartey, R. T. Evans, R. G. Caesar-TonThat, T. Caesar, A. J. TI Occurrence of spot-form net blotch of barley in the Mon-Dak area of the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lartey, R. T.; Evans, R. G.; Caesar-TonThat, T.; Caesar, A. J.] ARS, USDA, Sidney, MT USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 67 EP 67 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800353 ER PT J AU Lee, M Rogers, EE Stenger, DC AF Lee, M. Rogers, E. E. Stenger, D. C. TI A toxin-antitoxin system encoded by the Xylella fastidiosa chromosome regulates growth SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lee, M.; Rogers, E. E.; Stenger, D. C.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 67 EP 67 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800354 ER PT J AU Lee, RF Manjunath, K Ramadugu, C AF Lee, R. F. Manjunath, K. Ramadugu, C. TI Use of young indicator plants for biological indexing: Application to citrus certification programs SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lee, R. F.] ARS, Natl Clonal Germplasm Repository Citrus & Dates, USDA, Riverside, CA USA. [Manjunath, K.] ARS, USDA, Riverside, CA USA. [Ramadugu, C.] Univ Calif Riverside, Riverside, CA 92521 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 67 EP 68 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800356 ER PT J AU Li, S Ray, J Smith, J Frederick, R AF Li, S. Ray, J. Smith, J. Frederick, R. TI A new soybean rust resistance gene identified in PI 567102B SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Li, S.; Ray, J.; Smith, J.] ARS, USDA, Crop Genet Res Unit, Stoneville, MS USA. [Frederick, R.] ARS, USDA, Foreign Disease Weed Sci Res Unit, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 68 EP 69 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800362 ER PT J AU Li, S Jin, X Chen, J AF Li, S. Jin, X. Chen, J. TI Effects of piperidine alkaloids from the red imported fire ant on cucumber damping-off caused by Pythium ultimum in the greenhouse SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Li, S.] Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding, Hebei Province, Peoples R China. [Jin, X.; Chen, J.] ARS, USDA, MSA, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 68 EP 68 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800361 ER PT J AU Li, M Nam, J Jang, C Seo, E Song, J Jackson, AO Hammond, J Lim, H AF Li, M. Nam, J. Jang, C. Seo, E. Song, J. Jackson, A. O. Hammond, J. Lim, H. TI The host actin cytoskeleton is required for Barley stripe mosaic virus TGB3 cell wall localization SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Li, M.; Nam, J.; Jang, C.; Seo, E.; Song, J.; Lim, H.] Chungnam Natl Univ, Taejon, South Korea. [Jackson, A. O.] Univ Calif Berkeley, Berkeley, CA 94720 USA. [Hammond, J.] ARS, USDA, FNPRU, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 69 EP 70 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800368 ER PT J AU Li, R Fei, Z Ling, K AF Li, R. Fei, Z. Ling, K. TI Characterization and detection of Tomato necrotic stunt virus, a novel potyvirus infecting greenhouse tomatoes in Mexico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Li, R.; Ling, K.] ARS, USDA, US Vegetable Lab, Charleston, SC USA. [Fei, Z.] Cornell Univ, Boyce Thompson Inst Plant Res, USDA, ARS,Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 70 EP 70 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800370 ER PT J AU Li, Y Windham, M Trigiano, R Windham, A Reed, S Spiers, J Rinehart, T AF Li, Y. Windham, M. Trigiano, R. Windham, A. Reed, S. Spiers, J. Rinehart, T. TI Effects of light density on resistance of bigleaf hydrangea to Cercospora leaf spot SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Li, Y.] Connecticut Agr Expt Stn, New Haven, CT 06504 USA. [Windham, M.; Trigiano, R.; Windham, A.] Univ Tennessee, Knoxville, TN USA. [Reed, S.] ARS, USDA, Mcminnville, TN USA. [Spiers, J.] ARS, USDA, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 70 EP 70 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800372 ER PT J AU Lin, H Islam, MS Wen, A Gudmestad, N AF Lin, H. Islam, M. S. Wen, A. Gudmestad, N. TI Genotyping and population genetic analysis of 'Candidatus Liberibacter solanacearum', bacterium associated with potato zebra chip disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lin, H.] ARS, USDA, PWA, Parlier, CA USA. [Islam, M. S.] ARS, USDA, Parlier, CA USA. [Wen, A.; Gudmestad, N.] N Dakota State Univ, Fargo, ND 58105 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 71 EP 71 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800376 ER PT J AU Lin, H Nwugo, CC Duan, Y AF Lin, H. Nwugo, C. C. Duan, Y. TI Physiological and proteomic characterizations of 'Candidatus Liberibacter' associated diseases in citrus and potato plants SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lin, H.] ARS, USDA, PWA, Parlier, CA USA. [Nwugo, C. C.] ARS, USDA, Parlier, CA USA. [Duan, Y.] ARS, USDA, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 71 EP 71 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800375 ER PT J AU Lin, L Li, R Mock, R Kinard, G AF Lin, L. Li, R. Mock, R. Kinard, G. TI A multiplex TaqMan real-time RT-PCR assay for detection of Asian prunus viruses, Plum bark necrosis stem pitting associated virus, and Peach latent mosaic viroid SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lin, L.; Li, R.; Mock, R.; Kinard, G.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 71 EP 71 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800377 ER PT J AU Ling, K Li, R AF Ling, K. Li, R. TI Genetic diversity and phylogenetic analysis revealed Pepino mosaic virus in North America has shifted to Chilean genotypes SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ling, K.; Li, R.] ARS, USDA, US Vegetable Lab, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 71 EP 71 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800378 ER PT J AU Liu, Z Feng, S Pandey, MK Culbreath, A Guo, B AF Liu, Z. Feng, S. Pandey, M. K. Culbreath, A. Guo, B. TI Identification of expressed resistance gene analog (RGA) from peanut (Arachis hypogaea L.) expressed sequence tags (ESTs) and development of RGA-SSR marker SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Liu, Z.; Feng, S.; Pandey, M. K.; Culbreath, A.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Guo, B.] ARS, USDA, Crop Protect & Management Res Unit, Tifton, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 71 EP 72 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800379 ER PT J AU Liu, Z Chao, S Faris, JD Edwards, MC Friesen, TL AF Liu, Z. Chao, S. Faris, J. D. Edwards, M. C. Friesen, T. L. TI QTL mapping reveals effector-triggered susceptibility underlying the barley-Pyrenophora teres f. teres interaction SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Liu, Z.] N Dakota State Univ, Fargo, ND 58105 USA. [Chao, S.; Faris, J. D.; Edwards, M. C.; Friesen, T. L.] ARS, Cereal Crops Res Unit, No Crop Sci Lab, USDA, Fargo, ND USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 72 EP 72 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800380 ER PT J AU Lu, S Friesen, TL Faris, JD AF Lu, S. Friesen, T. L. Faris, J. D. TI Differential expression of the pathogenesis-related protein 1 (PR-1) gene family in stem rust (Puccinia graminis f. sp tritici)-wheat interactions SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lu, S.; Friesen, T. L.; Faris, J. D.] ARS, USDA, Cereal Crops Res Unit, Fargo, ND USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 73 EP 73 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800386 ER PT J AU Luo, W Gottwald, T Irey, MS AF Luo, W. Gottwald, T. Irey, M. S. TI Assessments of edge effect in intensity of HLB disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Luo, W.] ARS, USDA, Ft Pierce, FL USA. [Gottwald, T.] USDA, Ft Pierce, FL USA. [Irey, M. S.] US Sugar Corp, Southern Gardens Citrus, Clewiston, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 73 EP 73 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800388 ER PT J AU Luster, DG Carter, ML Peterson, GL McMahon, MB AF Luster, D. G. Carter, M. L. Peterson, G. L. McMahon, M. B. TI Repeating structure of internal transcribed spacer region 2 in Peronosclerospora spp. downy mildews SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Luster, D. G.; Carter, M. L.; Peterson, G. L.; McMahon, M. B.] ARS, USDA, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 73 EP 74 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800389 ER PT J AU Lynn, N Rowhani, A Sudarshana, MR AF Lynn, N. Rowhani, A. Sudarshana, M. R. TI Sequence analysis of the capsid protein of a Cherry leaf roll virus isolate causing blackline disease of walnuts in California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Lynn, N.; Sudarshana, M. R.] ARS, USDA, Davis, CA USA. [Rowhani, A.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 74 EP 74 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800390 ER PT J AU Ma, W Wen, X Gu, Y Deng, X Chen, J AF Ma, W. Wen, X. Gu, Y. Deng, X. Chen, J. TI Population diversity of 'Candidatus Liberibacter asiaticus' in southern China revealed by tandem repeat number variation in a hypervariable genomic locus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ma, W.; Wen, X.; Gu, Y.; Deng, X.] South China Agr Univ, Guangzhou, Guangdong, Peoples R China. [Chen, J.] ARS, USDA, PWA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 74 EP 74 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800391 ER PT J AU Maldonado, A AF Maldonado, A. TI Engineering plant defenses to broaden resistance in soybean to soybean cyst nematode SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Maldonado, A.] USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 75 EP 75 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800395 ER PT J AU Marasas, C AF Marasas, C. TI Integrated measures approaches as a pest risk management strategy for plants for planting: The case of Dracaena plants from Costa Rica SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Marasas, C.] USDA, APHIS, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 76 EP 76 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800400 ER PT J AU Martin, FN Zhang, Y Grunwald, N Cooke, DE Coffey, MD AF Martin, F. N. Zhang, Y. Grunwald, N. Cooke, D. E. Coffey, M. D. TI Expanded analysis of P. infestans mitochondrial haplotypes and correlation with nuclear genotype SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Martin, F. N.] ARS, USDA, Salinas, CA USA. [Zhang, Y.; Coffey, M. D.] Univ Calif Riverside, Riverside, CA 92521 USA. [Grunwald, N.] ARS, USDA, Corvallis, OR USA. [Cooke, D. E.] James Hutton Inst, Dundee, Scotland. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 76 EP 76 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800402 ER PT J AU Martin, FN Blair, JE Coffey, MD AF Martin, F. N. Blair, J. E. Coffey, M. D. TI Phylogeny of the genus Phytophthora estimated by multilocus analysis of mitochondrial genes and comparison with the nuclear phylogeny SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Martin, F. N.] ARS, USDA, Salinas, CA USA. [Blair, J. E.] Franklin & Marshall Coll, Lancaster, PA 17604 USA. [Coffey, M. D.] Univ Calif Riverside, Riverside, CA 92521 USA. NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 76 EP 76 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800401 ER PT J AU Marutani-Hert, M McClean, AE Maccree, MM Yakabe, LE Kluepfel, DA AF Marutani-Hert, M. McClean, A. E. Maccree, M. M. Yakabe, L. E. Kluepfel, D. A. TI Diversity of Agrobacterium tumefaciens found in California walnut-growing regions SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Marutani-Hert, M.; McClean, A. E.; Kluepfel, D. A.] ARS, USDA, Davis, CA USA. [Maccree, M. M.; Yakabe, L. E.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 76 EP 77 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800405 ER PT J AU Matthews, BF Abd El Kreem, RY AF Matthews, B. F. Abd El Kreem, R. Y. TI Posttranscriptional gene silencing of the gene encoding aldolase from soybean cyst nematode by transformed soybean roots SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Matthews, B. F.] ARS, USDA, Beltsville, MD USA. [Abd El Kreem, R. Y.] USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 77 EP 77 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800407 ER PT J AU Mazzola, M AF Mazzola, M. TI Replant disease control and soil system resilience to pathogen infestation in response to Brassicaceae seed meal amendment SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Mazzola, M.] ARS, USDA, Wenatchee, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 77 EP 77 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800410 ER PT J AU Mazzola, M Muramoto, J Shennan, C AF Mazzola, M. Muramoto, J. Shennan, C. TI Transformation of soil microbial community structure in response to anaerobic soil disinfestation for soilborne disease control in strawberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Mazzola, M.] ARS, USDA, Wenatchee, WA USA. [Muramoto, J.; Shennan, C.] Univ Calif Santa Cruz, Santa Cruz, CA 95064 USA. RI Shennan, Carol/I-1694-2013 OI Shennan, Carol/0000-0001-6401-5007 NR 0 TC 0 Z9 0 U1 2 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 77 EP 78 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800411 ER PT J AU McClean, AE Kluepfel, DA Aradyha, M Moersfelder, J Hackett, WP Dull, A Marsden, C AF McClean, A. E. Kluepfel, D. A. Aradyha, M. Moersfelder, J. Hackett, W. P. Dull, A. Marsden, C. TI Identification of sources of crown gall resistance in the Juglans germplasm SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [McClean, A. E.; Dull, A.] Univ Calif Davis, USDA, ARS, Davis, CA 95616 USA. [Kluepfel, D. A.] ARS, USDA, CPGRU, Davis, CA USA. [Aradyha, M.; Moersfelder, J.; Marsden, C.] ARS, USDA, Davis, CA USA. [Hackett, W. P.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 78 EP 78 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800414 ER PT J AU Medrano, EG Esquivel, JF AF Medrano, E. G. Esquivel, J. F. TI Vector-borne cotton boll disease transmitted by Nezara viridula nymphs SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Medrano, E. G.] ARS, USDA, CPRU, College Stn, TX USA. [Esquivel, J. F.] ARS, USDA, College Stn, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 79 EP 79 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800419 ER PT J AU Miao, H Di, D Lu, Y Redinbaugh, M Tian, L Zhang, A AF Miao, H. Di, D. Lu, Y. Redinbaugh, M. Tian, L. Zhang, A. TI Efficient inoculation of Rice black-streaked dwarf virus to maize using the planthopper Laodelphax striatellus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Miao, H.; Di, D.; Lu, Y.; Tian, L.; Zhang, A.] Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding, Peoples R China. [Redinbaugh, M.] Ohio State Univ, USDA ARS, Dept Plant Pathol, Wooster, OH USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 80 EP 80 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800426 ER PT J AU Munck, IA Burns, B Lombard, K Weimer, J Ostrofsky, WD AF Munck, I. A. Burns, B. Lombard, K. Weimer, J. Ostrofsky, W. D. TI What is causing white pine needle damage in northeastern North America? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Munck, I. A.] USDA Forest Serv, Durham, NH USA. [Burns, B.] Vermont Dept Forests Pk & Recreat, Springfield, VT USA. [Lombard, K.; Weimer, J.] New Hampshire Dept Nat Resources & Econ Dev, Hillsborough, NH USA. [Ostrofsky, W. D.] Maine Forest Serv, Augusta, ME USA. OI Munck, Isabel/0000-0001-6889-9196 NR 0 TC 2 Z9 2 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 84 EP 84 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800442 ER PT J AU Munoz, A Wang, S Rascoe, J AF Munoz, A. Wang, S. Rascoe, J. TI Survey for 'Candidatus Phytoplasma' species from nursery plants, urban shade trees, and agricultural crops in Nevada SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Munoz, A.; Wang, S.] Nevada Dept Agr, Sparks, NV USA. [Rascoe, J.] USDA APHIS PPQ PHP PSPI NIS, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 84 EP 84 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800443 ER PT J AU Musungu, BM Geisler, M Brown, R Bhatnagar, D Fakhoury, AM AF Musungu, B. M. Geisler, M. Brown, R. Bhatnagar, D. Fakhoury, A. M. TI A predicted interactome for Zea mays SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Musungu, B. M.; Fakhoury, A. M.] So Illinois Univ, Dept Plant Soil & Agr Syst, Carbondale, IL 62901 USA. [Geisler, M.] So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA. [Brown, R.; Bhatnagar, D.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. RI Geisler, Matt/A-4156-2008 OI Geisler, Matt/0000-0003-1888-4652 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 84 EP 84 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800446 ER PT J AU Nam, J Jang, C Li, M Kim, H Cho, S Kim, H Lakshman, DK Hammond, J Lim, H AF Nam, J. Jang, C. Li, M. Kim, H. Cho, S. Kim, H. Lakshman, D. K. Hammond, J. Lim, H. TI Alternanthera mosaic virus TGB1 interaction with chloroplast beta ATPase is necessary for viral replication SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Nam, J.; Jang, C.; Li, M.; Kim, H.; Cho, S.; Kim, H.; Lim, H.] Chungnam Natl Univ, Taejon, South Korea. [Lakshman, D. K.; Hammond, J.] USDA ARS FNPRU, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 85 EP 85 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800450 ER PT J AU Natilla, A Hammond, RW AF Natilla, A. Hammond, R. W. TI Selective RNA packaging of Maize rayado fino virus viruslike particles transiently expressed in Nicotiana benthamiana plants SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Natilla, A.; Hammond, R. W.] ARS, USDA, MPPL, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 85 EP 85 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800451 ER PT J AU Obasa, K St Amand, P Bai, G Kennelly, M AF Obasa, K. St Amand, P. Bai, G. Kennelly, M. TI Phenotypic and genotypic characterization of Rhizoctonia solani isolates from zoysiagrass in Kansas SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Obasa, K.; Kennelly, M.] Kansas State Univ, Manhattan, KS 66506 USA. [St Amand, P.; Bai, G.] ARS, USDA, Manhattan, KS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 86 EP 87 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800458 ER PT J AU Olatinwo, R Allison, J Streett, D Carlton, C AF Olatinwo, R. Allison, J. Streett, D. Carlton, C. TI Ambrosia beetle populations and associated fungal symbionts in central Louisiana SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Olatinwo, R.] Louisiana State Univ, Pineville, LA USA. [Allison, J.] Nat Resources Canada, Great Lakes Forestry Ctr, Sault Ste Marie, ON, Canada. [Streett, D.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA USA. [Carlton, C.] Louisiana State Univ, Dept Entomol, Baton Rouge, LA 70803 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 87 EP 88 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800461 ER PT J AU Olatinwo, R Allison, J Meeker, J Johnson, W Streett, D Carlton, C AF Olatinwo, R. Allison, J. Meeker, J. Johnson, W. Streett, D. Carlton, C. TI A target-specific approach for screening fungal symbionts of native Sirex populations SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Olatinwo, R.] Louisiana State Univ, Pineville, LA USA. [Allison, J.] Nat Resources Canada, Great Lakes Forestry Ctr, Sault Ste Marie, ON, Canada. [Meeker, J.; Johnson, W.] US Forest Serv, USDA, FHP R8, Pineville, LA USA. [Streett, D.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA USA. [Carlton, C.] Louisiana State Univ, Dept Entomol, Baton Rouge, LA 70803 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 87 EP 87 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800460 ER PT J AU Ortega-Beltran, A Callicott, KA Cotty, PJ AF Ortega-Beltran, A. Callicott, K. A. Cotty, P. J. TI Aspergillus flavus AF36 in Mexico: Distribution of an endemic biocontrol agent for mitigation of aflatoxin contamination of maize SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ortega-Beltran, A.] Univ Arizona, Sch Plant Sci, Tucson, AZ USA. [Callicott, K. A.; Cotty, P. J.] Univ Arizona, ARS, USDA, Sch Plant Sci, Tucson, AZ USA. NR 0 TC 1 Z9 1 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 89 EP 89 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800465 ER PT J AU Palumbo, JD O'Keeffe, TL Vasquez, SJ AF Palumbo, J. D. O'Keeffe, T. L. Vasquez, S. J. TI Effect of drying methods on incidence of mycotoxins and mycotoxigenic strains of Aspergillus section Nigri in California raisins SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Palumbo, J. D.; O'Keeffe, T. L.] ARS, USDA, WRRC, Albany, CA USA. [Vasquez, S. J.] Univ Calif Cooperat Extens, Fresno, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 89 EP 89 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800468 ER PT J AU Parker, JK Wisotsky, SR Sims, KR Hilf, ME De La Fuente, L AF Parker, J. K. Wisotsky, S. R. Sims, K. R. Hilf, M. E. De La Fuente, L. TI Survival of 'Candidatus Liberibacter asiaticus' in different media over time SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Parker, J. K.; Wisotsky, S. R.; De La Fuente, L.] Auburn Univ, Auburn, AL 36849 USA. [Sims, K. R.; Hilf, M. E.] ARS, USDA, Ft Pierce, FL USA. RI Parker, Jennifer/A-3273-2012 OI Parker, Jennifer/0000-0002-4382-0741 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 90 EP 90 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800470 ER PT J AU Pastalka, T Johnson-Brousseau, SA Kosta, KL Rooney-Latham, S Blomquist, C Suslow, K Bulluck, R Ghosh, S AF Pastalka, T. Johnson-Brousseau, S. A. Kosta, K. L. Rooney-Latham, S. Blomquist, C. Suslow, K. Bulluck, R. Ghosh, S. TI Tracking plant diseases by monitoring a sentinel plant system at the National Ornamentals Research Site at Dominican University of California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Pastalka, T.; Johnson-Brousseau, S. A.; Ghosh, S.] Dominican Univ Calif, San Rafael, CA USA. [Kosta, K. L.; Rooney-Latham, S.; Blomquist, C.] Calif Dept Food & Agr, Sacramento, CA 95814 USA. [Suslow, K.] Hines Growers LLC, NorCal, Winters, CA USA. [Bulluck, R.] USDA APHIS PPQ CPHST, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 90 EP 90 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800472 ER PT J AU Pastor-Corrales, M Shin, SH AF Pastor-Corrales, M. Shin, S. -H. TI Genetic analysis of broad-spectrum resistance in Mesoamerican common bean accession PI 310762 to the hypervariable bean rust pathogen SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Pastor-Corrales, M.; Shin, S. -H.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 90 EP 90 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800473 ER PT J AU Peduto, F Coop, LB Strand, JF Bettiga, LJ Broome, JC Mahaffee, WF Gubler, WD AF Peduto, F. Coop, L. B. Strand, J. F. Bettiga, L. J. Broome, J. C. Mahaffee, W. F. Gubler, W. D. TI Sustainable grape pest management for California using weather data and disease risk models SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Peduto, F.; Strand, J. F.; Broome, J. C.; Gubler, W. D.] Univ Calif Davis, Davis, CA 95616 USA. [Coop, L. B.] Oregon State Univ, Corvallis, OR 97331 USA. [Bettiga, L. J.] Univ Calif Cooperat Extens, Salinas, CA USA. [Mahaffee, W. F.] USDA ARS HCRL, Corvallis, OR USA. RI Hand, Francesca/D-1761-2013 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 91 EP 91 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800477 ER PT J AU Peter, KA Vico, I Gaskins, VL Janisiewicz, WJ Jurick, WM AF Peter, K. A. Vico, I. Gaskins, V. L. Janisiewicz, W. J. Jurick, W. M. TI Identification and characterization of two new Pencillium species causing blue mold of stored apple fruit in the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Peter, K. A.; Vico, I.; Gaskins, V. L.] USDA ARS, Beltsville, MD USA. [Janisiewicz, W. J.] USDA ARS, AFRS, Kearneysville, WV USA. [Jurick, W. M.] USDA ARS, Food Qual Lab, Beltsville, MD USA. NR 0 TC 1 Z9 1 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 92 EP 92 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800480 ER PT J AU Polashock, J Cai, G Hillman, B Oudemans, PV AF Polashock, J. Cai, G. Hillman, B. Oudemans, P. V. TI Draft genome assembly of the ascomycete Colletotrichum acutatum SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Polashock, J.] USDA ARS, Chatsworth, NJ USA. [Cai, G.; Hillman, B.] Rutgers State Univ, New Brunswick, NJ 08903 USA. [Oudemans, P. V.] Rutgers State Univ, Chatsworth, NJ USA. RI Cai, Guohong/N-1274-2013 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 93 EP 93 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800486 ER PT J AU Poromarto, SH Nelson, BD Goswami, RS Welsh, M AF Poromarto, S. H. Nelson, B. D. Goswami, R. S. Welsh, M. TI Reproduction of soybean cyst nematode on accessions of the core collection of Phaseolus vulgaris SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Poromarto, S. H.; Nelson, B. D.] N Dakota State Univ, Fargo, ND 58105 USA. [Goswami, R. S.] DuPont Crop Protect, Stine Haskell Res Ctr, Newark, DE USA. [Welsh, M.] Washington State Univ, USDA ARS, Western Reg Plant Intro Stn, Pullman, WA 99164 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 93 EP 94 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800490 ER PT J AU Qiu, D Vandemark, G Chen, W AF Qiu, D. Vandemark, G. Chen, W. TI Transcriptome analyses of the interaction between Sclerotinia trifoliorum and chickpea using RNA sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Qiu, D.] Washington State Univ, Pullman, WA 99164 USA. [Vandemark, G.; Chen, W.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 95 EP 96 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800499 ER PT J AU Qiu, D Vandemark, G Chen, W AF Qiu, D. Vandemark, G. Chen, W. TI Transcriptome analyses of Sclerotinia sclerotiorum infecting chickpea and lentil using RNA sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Qiu, D.] Washington State Univ, Pullman, WA 99164 USA. [Vandemark, G.; Chen, W.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 95 EP 95 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800498 ER PT J AU Quito-Avila, DF Martin, RR AF Quito-Avila, D. F. Martin, R. R. TI Blueberry necrotic ring blotch virus represents a unique genus of plant RNA viruses SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Quito-Avila, D. F.] Ctr Invest Biotecnol Ecuador CIBE ESPOL, Guayaquil, Ecuador. [Martin, R. R.] USDA ARS, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 96 EP 96 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800500 ER PT J AU Ramadugu, C Kayim, M Keremane, M Lee, RF AF Ramadugu, C. Kayim, M. Keremane, M. Lee, R. F. TI Development of an immunoassay for detection of Citrus psorosis virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ramadugu, C.] Univ Calif Riverside, Riverside, CA 92521 USA. [Kayim, M.] Cukurova Univ, Adana, Turkey. [Keremane, M.] USDA ARS, Riverside, CA USA. [Lee, R. F.] USDA ARS, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 97 EP 97 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800504 ER PT J AU Rappaport, K Bowman, H Elphinstone, J Levy, L Liu, Z AF Rappaport, K. Bowman, H. Elphinstone, J. Levy, L. Liu, Z. TI Evaluation of Ralstonia CANARY technology SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Rappaport, K.; Bowman, H.; Liu, Z.] USDA APHIS PPQ CPHST, Beltsville, MD USA. [Elphinstone, J.] Food & Environm Res Agcy, York, N Yorkshire, England. [Levy, L.] USDA APHIS, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 97 EP 98 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800507 ER PT J AU Reitz, SR Adkins, S AF Reitz, S. R. Adkins, S. TI Differential acquisition and transmission of Florida Tomato spotted wilt virus isolates by Western flower thrips SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Reitz, S. R.] USDA ARS, Tallahassee, FL USA. [Adkins, S.] USDA ARS USHRL, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 98 EP 98 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800511 ER PT J AU Roberts, D McKenna, LF Maul, JE Lakshman, DK Buyer, J Emche, SE AF Roberts, D. McKenna, L. F. Maul, J. E. Lakshman, D. K. Buyer, J. Emche, S. E. TI Natural products for suppression of damping-off pathogens in organic cucumber production SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Roberts, D.] USDA, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [McKenna, L. F.; Maul, J. E.; Lakshman, D. K.; Buyer, J.; Emche, S. E.] Sustainable Agr Syst Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 100 EP 100 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800519 ER PT J AU Roberts, SM Bjornson, ML Savchenko, T Roubtsova, TV Pye, MF Kasuga, T Lazarus, C Dehesh, K Bostock, RM AF Roberts, S. M. Bjornson, M. L. Savchenko, T. Roubtsova, T. V. Pye, M. F. Kasuga, T. Lazarus, C. Dehesh, K. Bostock, R. M. TI Eicosapolyenoic acid action in Arabidopsis and tomato: Novel PAMPs with reciprocal effects on plant defense signaling networks SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Roberts, S. M.; Bjornson, M. L.; Savchenko, T.; Roubtsova, T. V.; Pye, M. F.; Dehesh, K.; Bostock, R. M.] Univ Calif Davis, Davis, CA 95616 USA. [Kasuga, T.] Univ Calif Davis, USDA ARS, Davis, CA 95616 USA. [Lazarus, C.] Univ Bristol, Bristol, Avon, England. RI Savchenko, Tatyana/E-7091-2014 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 100 EP 100 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800520 ER PT J AU Robertson, NL Macknicki, CJ Steinlage, TA Postman, JD AF Robertson, N. L. Macknicki, C. J. Steinlage, T. A. Postman, J. D. TI Incidence and molecular detection of Gooseberry vein banding associated virus in the Ribes spp. collection of the National Clonal Germplasm Repository SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Robertson, N. L.; Macknicki, C. J.; Steinlage, T. A.] USDA ARS, Palmer, AK USA. [Postman, J. D.] USDA ARS, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 100 EP 101 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800521 ER PT J AU Robertson, AE Block, CC Hurburgh, CR Shepherd, LM AF Robertson, A. E. Block, C. C. Hurburgh, C. R. Shepherd, L. M. TI Effect of Goss's leaf blight severity on grain quality and on Clavibacter michiganensis subsp nebraskensis seed infection SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Robertson, A. E.; Hurburgh, C. R.] Iowa State Univ, Ames, IA USA. [Block, C. C.; Shepherd, L. M.] USDA ARS, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 101 EP 101 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800522 ER PT J AU Ross-Davis, A Stewart, J Hanna, J Kim, M Cronn, R Rai, H Richardson, B McDonald, G Klopfenstein, N AF Ross-Davis, A. Stewart, J. Hanna, J. Kim, M. Cronn, R. Rai, H. Richardson, B. McDonald, G. Klopfenstein, N. TI Transcriptome characterization of an Armillaria root disease pathogen reveals candidate pathogenicity-related genes SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ross-Davis, A.] US Forest Serv, USDA, Moscow, ID USA. [Stewart, J.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Hanna, J.; McDonald, G.; Klopfenstein, N.] USDA FS, Rocky Mt Res Stn, Moscow, ID USA. [Kim, M.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul, South Korea. [Cronn, R.] USDA FS, Pacific NW Res Stn, Corvallis, OR USA. [Rai, H.] Utah State Univ, Wildland Resources Dept, Logan, UT 84322 USA. [Richardson, B.] USDA FS, Rocky Mt Res Stn, Provo, UT USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 102 EP 103 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800532 ER PT J AU Roy, A Choudhary, N Leon, GA Achor, D Shao, J Wei, G Picton, DD Levy, L Nakhla, MK Hartung, JS Brlansky, RH AF Roy, A. Choudhary, N. Leon, G. A. Achor, D. Shao, J. Wei, G. Picton, D. D. Levy, L. Nakhla, M. K. Hartung, J. S. Brlansky, R. H. TI Identification of a new citrus cytoplasmic virus associated with citrus leprosis disease in Colombia using deep sequencing SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Roy, A.; Choudhary, N.; Achor, D.; Brlansky, R. H.] Univ Florida, CREC, Lake Alfred, FL USA. [Leon, G. A.] Ctr Invest La Libertad, Corpoica, Villavicencio, Colombia. [Shao, J.; Hartung, J. S.] USDA ARS, MPPL, Beltsville, MD USA. [Wei, G.; Picton, D. D.; Levy, L.; Nakhla, M. K.] USDA APHIS PPQ CPHST, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 103 EP 103 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800534 ER PT J AU Rubio, I Bouzar, H Jardini, TM Koike, ST Bull, CT AF Rubio, I. Bouzar, H. Jardini, T. M. Koike, S. T. Bull, C. T. TI Novel Pseudomonas syringae strains associated with leaf spot diseases on watermelon (Citrullus lanatus) and squash (Cucurbita pepo) in California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Rubio, I.] Calif State Univ Monterey, Salinas, CA USA. [Bouzar, H.] Sakata Seed Amer Inc, Salinas, CA USA. [Jardini, T. M.; Bull, C. T.] USDA ARS, Salinas, CA USA. [Koike, S. T.] Univ Calif Cooperat Extens, Salinas, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 103 EP 103 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800535 ER PT J AU Rush, TA Kennedy, B McTaggart, A Heller, G Toome, M Hartman, GL Schneider, RW Aime, MC AF Rush, T. A. Kennedy, B. McTaggart, A. Heller, G. Toome, M. Hartman, G. L. Schneider, R. W. Aime, M. C. TI Extent of variability of the internal transcribed spacer region within Phakopsora pachyrhizi SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Rush, T. A.; Kennedy, B.; McTaggart, A.; Heller, G.; Toome, M.; Schneider, R. W.; Aime, M. C.] Louisiana State Univ, Ctr Agr, Baton Rouge, LA 70803 USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. RI Toome, Merje/J-2678-2013 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 103 EP 103 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800536 ER PT J AU Saito, S Cadle-Davidson, L Wilcox, WF AF Saito, S. Cadle-Davidson, L. Wilcox, W. F. TI Comparative analysis of techniques for detection of quiescent Botrytis cinerea in grapes by quantitative PCR SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Saito, S.; Wilcox, W. F.] Cornell Univ NYSAES, Geneva, NY USA. [Cadle-Davidson, L.] USDA ARS, Grape Genet Res Unit, Geneva, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 104 EP 105 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800543 ER PT J AU Samac, D Dornbusch, M Foster-Hartnett, D Tu, Z Gantt, S AF Samac, D. Dornbusch, M. Foster-Hartnett, D. Tu, Z. Gantt, S. TI Role of a putative amino acid transporter in fungal disease resistance in alfalfa SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Samac, D.; Dornbusch, M.] USDA ARS, St Paul, MN USA. [Foster-Hartnett, D.; Tu, Z.; Gantt, S.] Univ Minnesota, St Paul, MN 55108 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 105 EP 105 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800545 ER PT J AU Schmidt, LS Bhat, RG Kluepfel, DA Browne, GT AF Schmidt, L. S. Bhat, R. G. Kluepfel, D. A. Browne, G. T. TI Resistance to Phytophthora in new rootstocks for almond and stone fruits SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Schmidt, L. S.; Kluepfel, D. A.; Browne, G. T.] USDA ARS, CPGRU, Davis, CA USA. [Bhat, R. G.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 106 EP 107 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800553 ER PT J AU Shiel, P Mavrodieva, V Dennis, G AF Shiel, P. Mavrodieva, V. Dennis, G. TI Proficiency test results summary for the 2011 National Plant Protection Laboratory Accreditation Program (NPPLAP) citrus greening (huanglongbing) detection assay SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Shiel, P.; Dennis, G.] USDA APHIS PPQ CPHST, Raleigh, NC USA. [Mavrodieva, V.] USDA APHIS PPQ CPHST, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 108 EP 108 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800563 ER PT J AU Shjerve, RA Liu, Z Faris, JD Rasmussen, JB Friesen, TL AF Shjerve, R. A. Liu, Z. Faris, J. D. Rasmussen, J. B. Friesen, T. L. TI Identification of multiple virulence QTL in Pyrenophora teres f. teres associated with net form net blotch in barley SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Shjerve, R. A.; Liu, Z.; Rasmussen, J. B.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58105 USA. [Faris, J. D.; Friesen, T. L.] USDA ARS, Cereal Crops Unit, Northern Crops Sci Lab, Fargo, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 108 EP 109 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800564 ER PT J AU Sikdar, P Okubara, P Mazzola, M Xiao, C AF Sikdar, P. Okubara, P. Mazzola, M. Xiao, C. TI PCR assays for diagnosis of postharvest fruit rots and early detection of Phacidiopycnis washingtonensis and Sphaeropsis pyriputrescens in apple fruit SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Sikdar, P.] Washington State Univ, Wenatchee, WA USA. [Okubara, P.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Mazzola, M.] USDA ARS, Wenatchee, WA USA. [Xiao, C.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 109 EP 110 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800569 ER PT J AU Simko, I Piepho, H AF Simko, I. Piepho, H. TI AUDPC and AUDPS: What is the difference? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Simko, I.] USDA ARS, Salinas, CA USA. [Piepho, H.] Univ Hohenheim, Stuttgart, Germany. RI Simko, Ivan/J-6048-2012 OI Simko, Ivan/0000-0002-8769-8477 NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 110 EP 110 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800571 ER PT J AU Skaria, M Satpute, A Kunta, M daGraca, J Perez, J Malik, N AF Skaria, M. Satpute, A. Kunta, M. daGraca, J. Perez, J. Malik, N. TI Recent studies on sweet orange scab (SOS) in Texas SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Skaria, M.; Satpute, A.; Kunta, M.; daGraca, J.] Texas A&M Univ Kingsville, Weslaco, TX USA. [Perez, J.; Malik, N.] USDA ARS, Weslaco, TX 78596 USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 110 EP 110 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800573 ER PT J AU Smith, BJ Miller-Butler, MA AF Smith, B. J. Miller-Butler, M. A. TI Botryosphaeria stem blight of southern blueberries: Effect of temperature on infection and lesion development SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Smith, B. J.; Miller-Butler, M. A.] USDA ARS, Thad Cochran Southern Hort Lab, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 111 EP 111 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800575 ER PT J AU Smith, DR Juzwik, J Stanosz, GR AF Smith, D. R. Juzwik, J. Stanosz, G. R. TI Seasonal variation in presence and abundance of inoculum of the Heterobasidion root disease pathogen in central Wisconsin SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Smith, D. R.; Stanosz, G. R.] Univ Wisconsin, Madison, WI USA. [Juzwik, J.] US Forest Serv, USDA, No Res Stn, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 111 EP 111 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800578 ER PT J AU Stewart, LR Jones, MW Haque, M Redinbaugh, MG AF Stewart, L. R. Jones, M. W. Haque, M. Redinbaugh, M. G. TI Determining resistance conferred by Wsm loci to Johnsongrass mosaic virus (JGMV) and Sorghum mosaic virus (SrMV) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Stewart, L. R.; Jones, M. W.; Redinbaugh, M. G.] ARS, USDA, Wooster, OH USA. [Haque, M.] Bangladesh Agr Univ, Mymensingh, Bangladesh. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 114 EP 114 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800596 ER PT J AU Stobbe, A Melcher, UK Fletcher, J Schneider, WL AF Stobbe, A. Melcher, U. K. Fletcher, J. Schneider, W. L. TI Validation of a unique sequence-based detection of plant pathogens using next-generation sequence data SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Stobbe, A.; Melcher, U. K.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Fletcher, J.] Oklahoma State Univ, Natl Inst Microbial Forens & Food & Agr Biosecur, Stillwater, OK 74078 USA. [Schneider, W. L.] ARS, USDA, FDWSRU, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 114 EP 115 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800597 ER PT J AU Stobbe, A Melcher, UK Schneider, WL AF Stobbe, A. Melcher, U. K. Schneider, W. L. TI Designing and validation of e-probes to strain type Plum pox virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Stobbe, A.; Melcher, U. K.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W. L.] ARS, USDA, FDWSRU, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 115 EP 115 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800598 ER PT J AU Strausbaugh, CA Eujayl, IA AF Strausbaugh, C. A. Eujayl, I. A. TI Influence of tillage systems on Rhizoctonia-bacterial root rot complex in sugar beet SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Strausbaugh, C. A.; Eujayl, I. A.] ARS, USDA, NWISRL, Kimberly, ID USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 115 EP 115 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800600 ER PT J AU Suthaparan, A Stensvand, A Solhaug, KA Torre, S Telfer, K Ruud, A Cadle-Davidson, L Mortensen, L Gadoury, DM Seem, RC Gislerod, HR AF Suthaparan, A. Stensvand, A. Solhaug, K. A. Torre, S. Telfer, K. Ruud, A. Cadle-Davidson, L. Mortensen, L. Gadoury, D. M. Seem, R. C. Gislerod, H. R. TI Suppression of cucumber powdery mildew by UV-B is affected by background light quality SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Suthaparan, A.; Torre, S.; Telfer, K.; Ruud, A.; Mortensen, L.; Gislerod, H. R.] Norwegian Univ Life Sci, As, Norway. [Stensvand, A.; Solhaug, K. A.] Bioforsk, As, Norway. [Cadle-Davidson, L.] USDA ARS, Grape Genet Res Unit, Geneva, NY USA. [Gadoury, D. M.; Seem, R. C.] New York State Agr Exptl Stn, Geneva, NY USA. NR 0 TC 3 Z9 3 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 116 EP 116 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800605 ER PT J AU Szabo, LJ AF Szabo, L. J. TI Development of a rapid molecular assay for the Ug99 race group of Puccinia graminis SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Szabo, L. J.] USDA ARS, St Paul, MN USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 117 EP 117 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800609 ER PT J AU Tatineni, S French, R AF Tatineni, S. French, R. TI Identification of distinct functions of Wheat streak mosaic virus coat protein in virion assembly and virus movement SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Tatineni, S.; French, R.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 118 EP 118 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800617 ER PT J AU Thies, JA Ariss, JJ Hassell, RL Levi, A AF Thies, J. A. Ariss, J. J. Hassell, R. L. Levi, A. TI Response of melon grafted on different cucurbit rootstocks to root-knot nematodes SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Thies, J. A.; Ariss, J. J.; Levi, A.] USDA ARS, US Vegetable Lab, Charleston, SC USA. [Hassell, R. L.] Clemson Univ, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 1 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 119 EP 119 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800621 ER PT J AU Veetil, TT Sabanadzovic, S Keller, KE Martin, RR Tzanetakis, IE AF Veetil, T. Thekke Sabanadzovic, S. Keller, K. E. Martin, R. R. Tzanetakis, I. E. TI Genetic variation of a novel ampelovirus in blackberry SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Veetil, T. Thekke; Tzanetakis, I. E.] Univ Arkansas, Dept Plant Pathol, Div Agr, Fayetteville, AR 72701 USA. [Sabanadzovic, S.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Keller, K. E.; Martin, R. R.] USDA ARS, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 119 EP 119 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800620 ER PT J AU Tseng, H Katawczik, M Mila, A AF Tseng, H. Katawczik, M. Mila, A. TI Comparison of Ralstonia solanacearum strains isolated from tobacco and vegetable crops in North Carolina SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Tseng, H.; Mila, A.] N Carolina State Univ, Raleigh, NC 27695 USA. [Katawczik, M.] ARS, USDA, N Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 122 EP 122 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800637 ER PT J AU Tubajika, KM AF Tubajika, K. M. TI The sanitary effect of alkyl dimethyl benzyl ammonium chloride on bean pods infected by Pseudomonas syringae pv. syringae SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Tubajika, K. M.] USDA APHIS PPQ CPHST, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 122 EP 122 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800638 ER PT J AU Turechek, W Webster, C Kousik, S Adkins, S AF Turechek, W. Webster, C. Kousik, S. Adkins, S. TI Use of latent class analysis to estimate the sensitivities and specificities of diagnostic tests for Squash vein yellowing virus in cucurbits SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Turechek, W.; Webster, C.; Adkins, S.] ARS, USDA, USHRL, Ft Pierce, FL USA. [Kousik, S.] ARS, USDA, USVL, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 122 EP 122 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800639 ER PT J AU Twizeyimana, M Hartman, GL AF Twizeyimana, M. Hartman, G. L. TI In vitro Phakopsora pachyrhizi isolate sensitivity to fungicides and effect of fungicide and timing of application on soybean rust severity SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Twizeyimana, M.] Univ Calif Riverside, Riverside, CA 92521 USA. [Hartman, G. L.] Univ Illinois, USDA, ARS, Urbana, IL 61801 USA. [Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 122 EP 123 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800641 ER PT J AU Uppala, S Wu, B Alderman, SC AF Uppala, S. Wu, B. Alderman, S. C. TI Effects of preconditioning cold treatments and incubation temperature on germination of ergot bodies of Claviceps purpurea from Kentucky bluegrass SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Uppala, S.; Wu, B.] Oregon State Univ, Madras, OR USA. [Alderman, S. C.] ARS, USDA, NFSPRC, Corvallis, OR USA. NR 0 TC 1 Z9 1 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 123 EP 123 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800643 ER PT J AU Vahling-Armstrong, CM Humann, JL Lupien, S Dugan, F du Toit, LJ Schroeder, BK AF Vahling-Armstrong, C. M. Humann, J. L. Lupien, S. Dugan, F. du Toit, L. J. Schroeder, B. K. TI DNA macroarray for the detection of fungal onion bulb rot pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Vahling-Armstrong, C. M.; Humann, J. L.; Schroeder, B. K.] Washington State Univ, Pullman, WA 99164 USA. [Lupien, S.; Dugan, F.] ARS, USDA, Pullman, WA USA. [du Toit, L. J.] Washington State Univ, Mt Vernon, WA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 125 EP 125 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800650 ER PT J AU Valverde, R Okada, R Sabanadzovic, S Pastor-Corrales, M Fukuhara, T Moriyama, H AF Valverde, R. Okada, R. Sabanadzovic, S. Pastor-Corrales, M. Fukuhara, T. Moriyama, H. TI Endornaviruses in common bean (Phaseolus vulgaris) germplasm SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Valverde, R.] Louisiana State Univ, Ctr Agr, Dept Plant Pathol & Crop Physiol, Baton Rouge, LA 70803 USA. [Okada, R.; Fukuhara, T.; Moriyama, H.] Tokyo Univ Agr & Technol, Fac Agr, Mol & Cellular Biol Lab, Tokyo, Japan. [Sabanadzovic, S.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Pastor-Corrales, M.] USDA ARS, Beltsville Agr Res Ctr, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RI MORIYAMA, Hiromitsu/F-9256-2013; Fukuhara, Toshiyuki/C-3208-2013 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 126 EP 126 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800654 ER PT J AU Vico, I Peter, KA Gaskins, VL Janisieswicz, WJ Jurick, WM AF Vico, I. Peter, K. A. Gaskins, V. L. Janisieswicz, W. J. Jurick, W. M. TI Characterization of Botryosphaeria dothidea isolates causing postharvest decay on apple fruit SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Vico, I.; Peter, K. A.; Gaskins, V. L.] USDA ARS, Beltsville, MD USA. [Janisieswicz, W. J.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Jurick, W. M.] USDA ARS, Food Qual Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 127 EP 127 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800663 ER PT J AU Vittal, R Haudenshield, JS Hartman, GL AF Vittal, R. Haudenshield, J. S. Hartman, G. L. TI A multiplexed immunofluorescence method identifies Phakopsora pachyrhizi urediniospores and determines their viability SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Vittal, R.] Univ Illinois, Urbana, IL 61801 USA. [Haudenshield, J. S.] USDA ARS, Urbana, IL USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 128 EP 128 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800669 ER PT J AU Walkinshaw, CH Otrosina, WJ AF Walkinshaw, C. H. Otrosina, W. J. TI Normal destruction of cortical tissue in pine roots SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Walkinshaw, C. H.; Otrosina, W. J.] US Forest Serv, USDA, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 129 EP 129 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800671 ER PT J AU Wallis, C Rashed, A Rush, CM AF Wallis, C. Rashed, A. Rush, C. M. TI Large-scale shifts in potato (Solanum tuberosum) tuber physiology occur following infection by 'Candidatus Liberibacter solanacearum' SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wallis, C.] USDA ARS, Parlier, CA USA. [Rashed, A.; Rush, C. M.] Texas AgriLife Res, Bushland, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 129 EP 130 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800674 ER PT J AU Wallis, C Chen, J AF Wallis, C. Chen, J. TI Grapevines undergo varying shifts in secondary metabolic profiles when infected with Xylella fastidiosa SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wallis, C.; Chen, J.] USDA ARS, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 129 EP 129 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800673 ER PT J AU Wan, A Chen, X AF Wan, A. Chen, X. TI Stripe rust epidemics of wheat and barley and races of Puccinia striiformis identified in the United States in 2011 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wan, A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 3 Z9 3 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 130 EP 130 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800676 ER PT J AU Wang, M Abad, J Li, R AF Wang, M. Abad, J. Li, R. TI Biological and molecular characterization of a distinct genetic variant of Sweet potato virus G SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wang, M.; Li, R.] USDA ARS, Beltsville, MD USA. [Abad, J.] USDA, APHIS, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 130 EP 130 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800679 ER PT J AU Wang, J Vidalakis, G Lee, RF Yokomi, RK AF Wang, J. Vidalakis, G. Lee, R. F. Yokomi, R. K. TI Development of high-throughput assays for rapid and accurate 10-plex detection of citrus pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wang, J.; Vidalakis, G.] Univ Calif Riverside, Riverside, CA 92521 USA. [Lee, R. F.] USDA ARS, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. [Yokomi, R. K.] USDA ARS, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 131 EP 131 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800681 ER PT J AU Wang, M Wan, A Chen, X AF Wang, M. Wan, A. Chen, X. TI Genetic characterization of virulence/avirulence genes of Puccinia striiformis f. sp tritici SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wang, M.; Wan, A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 132 EP 132 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800689 ER PT J AU Wang, M Wan, A Chen, X AF Wang, M. Wan, A. Chen, X. TI Barberry does not play a role for stripe rust in the US Pacific Northwest SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wang, M.; Wan, A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 132 EP 132 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800688 ER PT J AU Weaver, MA Abbas, H Sciumbato, G Pringle, H Allen, T AF Weaver, M. A. Abbas, H. Sciumbato, G. Pringle, H. Allen, T. TI Aflatoxin management in corn with Afla-Guard SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Weaver, M. A.] USDA ARS BCPRU, Stoneville, MS USA. [Abbas, H.] USDA ARS, Stoneville, MS 38776 USA. [Sciumbato, G.; Pringle, H.; Allen, T.] Mississippi State Univ, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 133 EP 133 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800693 ER PT J AU Webster, CG Kousik, CS Hassell, RL Ling, KS Turechek, WW Adkins, S AF Webster, C. G. Kousik, C. S. Hassell, R. L. Ling, K. S. Turechek, W. W. Adkins, S. TI Virus movement within grafted watermelon plants SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Webster, C. G.; Turechek, W. W.; Adkins, S.] USDA ARS, US Hort Res Lab, Ft Pierce, FL USA. [Kousik, C. S.; Ling, K. S.] USDA ARS, US Vegetable Lab, Charleston, SC USA. [Hassell, R. L.] Clemson Univ, CREC, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 133 EP 133 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800694 ER PT J AU Webster, CG Reitz, SR Frantz, G Mellinger, HC Funderburk, J Adkins, S AF Webster, C. G. Reitz, S. R. Frantz, G. Mellinger, H. C. Funderburk, J. Adkins, S. TI Thrips transmission of a tospovirus reassortant SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Webster, C. G.; Adkins, S.] USDA ARS, US Hort Res Lab, Ft Pierce, FL USA. [Reitz, S. R.] USDA ARS, Ctr Med Agr & Vet Entomol, Tallahassee, FL USA. [Frantz, G.; Mellinger, H. C.] Glades Crop Care, Jupiter, FL USA. [Funderburk, J.] Univ Florida, North Florida Res & Educ Ctr, Quincy, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 133 EP 133 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800696 ER PT J AU Wechter, W Kousik, C McMillan, M Farnham, M Levi, A AF Wechter, W. Kousik, C. McMillan, M. Farnham, M. Levi, A. TI Three improved Citrullus lanatus var. citroides lines USVL246-FR2, USVL252-FR2, and USVL335-FR2, with resistance to Fusarium oxysporum f. sp niveum race 2 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wechter, W.; Kousik, C.; McMillan, M.; Farnham, M.; Levi, A.] USDA ARS, Charleston, SC USA. NR 0 TC 2 Z9 2 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 133 EP 133 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800697 ER PT J AU Wegulo, S Byamukama, E Tatineni, S Hein, GL Graybosch, RA Baenziger, PS AF Wegulo, S. Byamukama, E. Tatineni, S. Hein, G. L. Graybosch, R. A. Baenziger, P. S. TI Effects of single and double infections of winter wheat by Triticum mosaic virus and Wheat streak mosaic virus on grain yield and yield components SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wegulo, S.; Byamukama, E.; Hein, G. L.; Baenziger, P. S.] Univ Nebraska, Lincoln, NE USA. [Tatineni, S.; Graybosch, R. A.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. RI Baenziger, Peter/C-6490-2014 OI Baenziger, Peter/0000-0002-9109-6954 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 134 EP 134 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800700 ER PT J AU Wen, L Hartman, GL Eastburn, DM AF Wen, L. Hartman, G. L. Eastburn, D. M. TI Suppression of soybean diseases through the use of cover crops SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wen, L.; Eastburn, D. M.] Univ Illinois, Urbana, IL 61801 USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 134 EP 134 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800703 ER PT J AU West, ED Bowen, CR Haudenshield, JS Hartman, GL AF West, E. D. Bowen, C. R. Haudenshield, J. S. Hartman, G. L. TI Evaluation of pathogen and pest resistance in select commercial soybean cultivars from 1923 to 2007 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [West, E. D.; Haudenshield, J. S.] Univ Illinois, Champaign, IL USA. [Bowen, C. R.] USDA ARS, Urbana, IL USA. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 134 EP 135 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800704 ER PT J AU Wilson, A AF Wilson, A. TI Development of an electronic-nose technology for the rapid detection of agricultural pesticide residues SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wilson, A.] US Forest Serv, USDA, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 136 EP 136 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800711 ER PT J AU Wilson, A AF Wilson, A. TI Relative potential of major root-rot and bole-rot fungi to decay sapwood in landscape trees of southern temperate regions SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wilson, A.] US Forest Serv, USDA, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 136 EP 136 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800710 ER PT J AU Windels, CE Brantner, JR Campbell, LG Fugate, KK AF Windels, C. E. Brantner, J. R. Campbell, L. G. Fugate, K. K. TI Postharvest respiration rate and sucrose concentration of Rhizoctonia-infected sugar beet roots SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Windels, C. E.; Brantner, J. R.] Univ Minnesota, Northwest Res & Outreach Ctr, Crookston, MN USA. [Campbell, L. G.; Fugate, K. K.] USDA ARS, No Crop Sci Lab, Fargo, ND 58105 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 136 EP 136 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800712 ER PT J AU Wintermantel, WM Simko, I AF Wintermantel, W. M. Simko, I. TI Environmental factors contributing to development of lettuce dieback disease and genomic characterization of Lettuce necrotic stunt virus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wintermantel, W. M.; Simko, I.] USDA ARS, Salinas, CA USA. RI Simko, Ivan/J-6048-2012 OI Simko, Ivan/0000-0002-8769-8477 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 136 EP 136 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800714 ER PT J AU Wintermantel, WM McCreight, JD AF Wintermantel, W. M. McCreight, J. D. TI Enhanced resistance to CYSDV in melon (Cucumis melo L.) and identification of significant reservoir hosts for virus transmission in the southwestern United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wintermantel, W. M.; McCreight, J. D.] USDA ARS, Salinas, CA USA. NR 0 TC 0 Z9 0 U1 3 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 136 EP 137 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800715 ER PT J AU Wolfenbarger, SN Twomey, MC Gent, DH AF Wolfenbarger, S. N. Twomey, M. C. Gent, D. H. TI Distribution of mating type of Podosphaera macularis in the Pacific Northwest SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wolfenbarger, S. N.; Twomey, M. C.; Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 137 EP 137 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800717 ER PT J AU Wolfenbarger, SN Nelson, ME Grove, GG Woods, JL Gent, DH AF Wolfenbarger, S. N. Nelson, M. E. Grove, G. G. Woods, J. L. Gent, D. H. TI Characterization of ontogenic resistance to powdery mildew in hop cones SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Wolfenbarger, S. N.; Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Nelson, M. E.; Grove, G. G.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Plant Pathol, Prosser, WA 99350 USA. [Woods, J. L.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, USDA ARS, Forage Seed & Cereal Res Unit, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 137 EP 137 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800716 ER PT J AU Worrall, JJ Hogg, EH Rehfeldt, GE Hamann, A Michaelian, M Gray, L AF Worrall, J. J. Hogg, E. H. Rehfeldt, G. E. Hamann, A. Michaelian, M. Gray, L. TI Recent mortality episodes of Populus tremuloides and climate in North America SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Worrall, J. J.] US Forest Serv, Gunnison, CO USA. [Hogg, E. H.; Michaelian, M.] Nat Resources Canada, Canadian Forest Serv, Edmonton, AB, Canada. [Rehfeldt, G. E.] US Forest Serv, Moscow, ID USA. [Gray, L.] Univ Alberta, Dept Renewable Resources, Edmonton, AB, Canada. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 137 EP 137 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800720 ER PT J AU Xiang, Y Hartman, GL AF Xiang, Y. Hartman, G. L. TI Optimization of growing conditions to enhance phytotoxin production in cultural filtrates of Fusarium virguliforme, the causeof soybean sudden death syndrome SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Xiang, Y.] Univ Illinois, Urbana, IL 61801 USA. [Hartman, G. L.] ARS, USDA, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 139 EP 139 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800727 ER PT J AU Yang, H Haudenshield, JS Hartman, GL AF Yang, H. Haudenshield, J. S. Hartman, G. L. TI First report of Colletotrichum chlorophyti and a new Colletotrichum species causing soybean anthracnose SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Yang, H.; Haudenshield, J. S.] Univ Illinois, Urbana, IL 61801 USA. [Hartman, G. L.] Univ Illinois, USDA, ARS, Urbana, IL 61801 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 140 EP 140 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800734 ER PT J AU Yin, C Hulbert, S Schroeder, KL Mavrodi, O Mavrodi, D Schillinger, W Paulitz, TC AF Yin, C. Hulbert, S. Schroeder, K. L. Mavrodi, O. Mavrodi, D. Schillinger, W. Paulitz, T. C. TI Natural suppression of Rhizoctonia root rot by soil microbial communities in wheat from a Rhizoctonia decline site SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Yin, C.; Hulbert, S.; Schroeder, K. L.; Mavrodi, O.; Mavrodi, D.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Schillinger, W.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Paulitz, T. C.] ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA USA. RI Mavrodi, Dmitri/J-2745-2013 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 141 EP 141 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800737 ER PT J AU Yokomi, R Sisterson, M AF Yokomi, R. Sisterson, M. TI Estimation of incidence and spatial temporal distribution of citrus stubborn disease SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Yokomi, R.] ARS, USDA, PWA, Parlier, CA USA. [Sisterson, M.] ARS, USDA, PWA, SJVASC,CDPG, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 141 EP 141 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800739 ER PT J AU Zhang, M Powell, CA Guo, Y Duan, Y AF Zhang, M. Powell, C. A. Guo, Y. Duan, Y. TI Chemotherapy for citrus huanglongbing disease in the field SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Zhang, M.] Univ Florida, Ft Pierce, FL USA. [Powell, C. A.; Guo, Y.] Univ Florida, Indian River Res & Educ Ctr, IFAS, Ft Pierce, FL USA. [Duan, Y.] ARS, US Hort Lab, USDA, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 143 EP 143 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800747 ER PT J AU Zhao, Y Lee, I Wei, W Shao, J Suo, X Davis, RE AF Zhao, Y. Lee, I. Wei, W. Shao, J. Suo, X. Davis, R. E. TI The iPhyClassifier II: An update to the online tool for phytoplasma identification and classification SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Zhao, Y.; Lee, I.; Wei, W.; Shao, J.; Suo, X.; Davis, R. E.] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 143 EP 143 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800750 ER PT J AU Zhou, L Powell, CA Li, W Duan, Y AF Zhou, L. Powell, C. A. Li, W. Duan, Y. TI Evolving diversity of 'Candidatus Liberibacter asiaticus' mediated by frequent recombination and reassortment of its prophages SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Zhou, L.] Univ Florida, Indian River Res & Educ Ctr, USDA, ARS, Ft Pierce, FL USA. [Powell, C. A.] Univ Florida, Indian River Res & Educ Ctr, IFAS, Ft Pierce, FL USA. [Li, W.] USDA, Natl Plant Germplasm & Biotechnol Lab, APHIS, PPQ,CPHST, Beltsville, MD 20705 USA. [Duan, Y.] ARS, USDA, USHRL, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 144 EP 144 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800752 ER PT J AU Zhu, H Ozkan, E Derksen, RD Reding, ME Ranger, CM Canas, L Krause, CR Locke, JC Ernst, SC Zondag, RH Fulcher, A Rosetta, R Jeon, H Chen, Y Gu, J Liu, H Shen, Y Rios, AA AF Zhu, H. Ozkan, E. Derksen, R. D. Reding, M. E. Ranger, C. M. Canas, L. Krause, C. R. Locke, J. C. Ernst, S. C. Zondag, R. H. Fulcher, A. Rosetta, R. Jeon, H. Chen, Y. Gu, J. Liu, H. Shen, Y. Rios, A. A. TI Development of smart spray systems to enhance delivery of pesticides in field nursery production SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Zhu, H.; Derksen, R. D.; Reding, M. E.; Ranger, C. M.; Krause, C. R.; Jeon, H.] ARS, USDA, ATRU, Wooster, OH USA. [Ozkan, E.; Ernst, S. C.; Zondag, R. H.] Ohio State Univ, Columbus, OH 43210 USA. [Canas, L.; Chen, Y.; Liu, H.; Shen, Y.; Rios, A. A.] Ohio State Univ, Wooster, OH USA. [Locke, J. C.] ARS, USDA, ATRU, Toledo, OH USA. [Fulcher, A.] Univ Tennessee, Knoxville, TN USA. [Rosetta, R.] Oregon State Univ, North Willamette Res & Extens Ctr, Aurora, OR USA. [Gu, J.] Nanjing Agr Univ, Coll Engn, Nanjing, Jiangsu, Peoples R China. RI Ernst, Stanley/J-9388-2012 NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 144 EP 145 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800755 ER PT J AU Zurn, J Newcomb, M Rouse, M Jin, Y Chao, S Sthapit, J See, D Wanyera, R Njau, P Bonman, J Brueggeman, R Acevedo, M AF Zurn, J. Newcomb, M. Rouse, M. Jin, Y. Chao, S. Sthapit, J. See, D. Wanyera, R. Njau, P. Bonman, J. Brueggeman, R. Acevedo, M. TI High resolution mapping of a Ug99 resistance gene from a spring wheat landrace SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Zurn, J.; Brueggeman, R.; Acevedo, M.] N Dakota State Univ, Fargo, ND 58105 USA. [Newcomb, M.; Bonman, J.] ARS, USDA, Aberdeen, ID USA. [Rouse, M.; Jin, Y.] ARS, USDA, St Paul, MN USA. [Chao, S.] ARS, Cereal Crops Res Unit, No Crop Sci Lab, USDA, Fargo, ND USA. [Sthapit, J.; See, D.] Washington State Univ, Pullman, WA 99164 USA. [Wanyera, R.; Njau, P.] Kenya Agr Res Inst, Njoro, Kenya. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 145 EP 145 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800758 ER PT J AU Kistler, HC AF Kistler, H. C. TI A toxic character: Fusarium graminearum and mycotoxin biosynthesis SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Kistler, H. C.] ARS, USDA, Cereal Dis Lab, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 146 EP 146 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800759 ER PT J AU Muehlbauer, GJ Shin, S Boddu, J Gardiner, S Jia, H Cho, S McCormick, S Schweiger, W Lemmens, M Berthiller, F Hametner, C Paris, PK Torres-Acosta, J Adam, G AF Muehlbauer, G. J. Shin, S. Boddu, J. Gardiner, S. Jia, H. Cho, S. McCormick, S. Schweiger, W. Lemmens, M. Berthiller, F. Hametner, C. Paris, P. Kovalsky Torres-Acosta, J. Adam, G. TI The role of trichothecenes in the Triticeae-Fusarium graminearum interactions SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Muehlbauer, G. J.; Shin, S.; Boddu, J.; Gardiner, S.; Jia, H.; Cho, S.] Univ Minnesota, St Paul, MN 55108 USA. [McCormick, S.] ARS, USDA, Peoria, IL USA. [Schweiger, W.; Lemmens, M.; Berthiller, F.; Paris, P. Kovalsky; Torres-Acosta, J.; Adam, G.] Univ Nat Resources & Appl Life Sci, Tulln, Austria. [Hametner, C.] Vienna Univ Technol, A-1040 Vienna, Austria. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 146 EP 147 PG 2 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800762 ER PT J AU Roper, M Rapicavoli, J Clifford, J AF Roper, M. Rapicavoli, J. Clifford, J. TI The role of the cell surface lipopolysaccharide molecule in Xylella fastidiosa biofilm formation and virulence in the grapevine host SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Roper, M.; Rapicavoli, J.] Univ Calif Riverside, Riverside, CA 92521 USA. [Clifford, J.] ARS, USDA, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 147 EP 147 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800767 ER PT J AU Jansky, SH Cai, X AF Jansky, S. H. Cai, X. TI Breeding potato for PVY resistance SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Jansky, S. H.] ARS, USDA, Madison, WI USA. [Jansky, S. H.] Univ Wisconsin, Madison, WI USA. [Cai, X.] Huazhong Agr Univ, Coll Hort & Forestry, Wuhan, Peoples R China. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 148 EP 148 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800771 ER PT J AU Ganiger, M Walker, DR Chen, Z AF Ganiger, M. Walker, D. R. Chen, Z. TI Proteomics-based study of host-fungus interaction between soybean and Phakopsora pachyrhizi using recombinant inbred line (RIL)-derived sister lines SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ganiger, M.; Chen, Z.] Louisiana State Univ, Baton Rouge, LA 70803 USA. [Walker, D. R.] ARS, USDA, Soybean Maize Germplasm Pathol & Genet Res Unit, Urbana, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 150 EP 150 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800783 ER PT J AU Spaine, P Cordts, JM AF Spaine, P. Cordts, J. M. TI Current processes involved in biotech crop deregulation SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Spaine, P.; Cordts, J. M.] Biotechnol Regulatory Serv, USDA, APHIS, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 152 EP 152 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800790 ER PT J AU Burelle, NK Rosskopf, EN Bausher, MG McCollum, GG AF Burelle, N. K. Rosskopf, E. N. Bausher, M. G. McCollum, G. G. TI Grafting as a production system component for nematode management in Florida vegetables SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Burelle, N. K.; Rosskopf, E. N.; Bausher, M. G.; McCollum, G. G.] ARS, USDA, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 153 EP 153 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800798 ER PT J AU Rudyj, ES AF Rudyj, E. S. TI The National Clean Plant Network SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Rudyj, E. S.] USDA, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 154 EP 154 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800802 ER PT J AU Bagewadi, B Henderson, DC Fischer, K Jordan, RL Wang, D Perry, KL Melcher, U Hammond, J Fauquet, CM AF Bagewadi, B. Henderson, D. C. Fischer, K. Jordan, R. L. Wang, D. Perry, K. L. Melcher, U. Hammond, J. Fauquet, C. M. TI All plant virus chip: Shifting from proof to use SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Bagewadi, B.; Fauquet, C. M.] Danforth Plant Sci Ctr, St Louis, MO USA. [Henderson, D. C.] ARS, USDA, Beltsville, MD USA. [Fischer, K.] Univ Utah, Sch Med, Salt Lake City, UT USA. [Jordan, R. L.] ARS, USDA, BA, Mol Plant Pathol Lab, Beltsville, MD USA. [Wang, D.] Washington Univ, Sch Med, St Louis, MO USA. [Perry, K. L.] Cornell Univ, Ithaca, NY USA. [Melcher, U.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Hammond, J.] ARS, USDA, Floral & Nursery Plants Res Unit, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 155 EP 155 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800809 ER PT J AU Schneider, WL Stobbe, AH Daniels, J Espindola, AS Verma, R Blagden, T Fletcher, J Ochoa-Corona, F Garzon, C Hoyt, PR Melcher, U AF Schneider, W. L. Stobbe, A. H. Daniels, J. Espindola, A. S. Verma, R. Blagden, T. Fletcher, J. Ochoa-Corona, F. Garzon, C. Hoyt, P. R. Melcher, U. TI Next-generation diagnostics: Eliminating the excessive sequence processing associated with next-generation sequencing using EDNA SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Schneider, W. L.] ARS, USDA, FDWSRU, Ft Detrick, MD USA. [Stobbe, A. H.; Daniels, J.; Espindola, A. S.; Verma, R.; Blagden, T.; Fletcher, J.; Ochoa-Corona, F.; Garzon, C.; Hoyt, P. R.; Melcher, U.] Oklahoma State Univ, Stillwater, OK 74078 USA. RI Espindola, Andres/A-5056-2016 OI Espindola, Andres/0000-0002-9658-0673 NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 155 EP 155 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800808 ER PT J AU Levy, L AF Levy, L. TI Pathogen signatures-Beyond nucleic acids & proteins SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Levy, L.] USDA APHIS PPQ CPHST, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 156 EP 156 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800812 ER PT J AU Liu, Z Bowman, H Rappaport, K Levy, L AF Liu, Z. Bowman, H. Rappaport, K. Levy, L. TI CANARY: Serological detection sees a new dawn SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Liu, Z.; Bowman, H.; Rappaport, K.] USDA APHIS PPQ CPHST, Beltsville, MD USA. [Levy, L.] USDA APHIS PPQ CPHST, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 156 EP 156 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800811 ER PT J AU Sudarshana, MR AF Sudarshana, M. R. TI Isothermal amplification: So many names, are there differences? SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Sudarshana, M. R.] ARS, USDA, Davis, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 156 EP 156 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800813 ER PT J AU Funnell-Harris, DL Sattler, SE Prom, LK Dowd, PF Pedersen, JF AF Funnell-Harris, D. L. Sattler, S. E. Prom, L. K. Dowd, P. F. Pedersen, J. F. TI Response of sorghum modified for bioenergy to grain and stalk fungal pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Funnell-Harris, D. L.; Sattler, S. E.; Pedersen, J. F.] ARS, USDA, Lincoln, NE USA. [Prom, L. K.] ARS, USDA, College Stn, TX USA. [Dowd, P. F.] ARS, USDA, Peoria, IL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 157 EP 157 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800819 ER PT J AU Uppalapati, SR Ishiga, Y Serba, D Szabo, LJ Saha, MC Mysore, KS AF Uppalapati, S. R. Ishiga, Y. Serba, D. Szabo, L. J. Saha, M. C. Mysore, K. S. TI Hunt for sources of rust resistance in the bioenergy crop, switchgrass (Panicum virgatum L.) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Uppalapati, S. R.; Ishiga, Y.; Serba, D.; Saha, M. C.; Mysore, K. S.] Samuel Roberts Noble Fdn Inc, Ardmore, OK USA. [Szabo, L. J.] ARS, Cereal Dis Lab, USDA, St Paul, MN USA. NR 0 TC 0 Z9 0 U1 0 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 157 EP 157 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800818 ER PT J AU Seybold, SJ AF Seybold, S. J. TI Life history of the walnut twig beetle, Pityophthorus juglandis, and its association with Geosmithia morbida, causal agent of thousand cankers disease in the United States SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Seybold, S. J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 159 EP 159 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800827 ER PT J AU Gottwald, TR Riley, TD Irey, MS Parnell, SR AF Gottwald, T. R. Riley, T. D. Irey, M. S. Parnell, S. R. TI Transportation grids as early indicators and warning-The use of census and travel data for prediction of disease incursions SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Gottwald, T. R.] USDA ARS, Ft Pierce, FL USA. [Riley, T. D.] USDA APHIS PPQ, Orlando, FL USA. [Irey, M. S.] US Sugar Corp, Clewiston, FL USA. [Parnell, S. R.] Rothamsted Res, Harpenden, Herts, England. RI parnell, stephen/I-7682-2015 OI parnell, stephen/0000-0002-2625-4557 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 161 EP 161 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800840 ER PT J AU Berger, PH Brown, LG AF Berger, P. H. Brown, L. G. TI Even when data are fluid a decision must be made SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Berger, P. H.; Brown, L. G.] USDA APHIS PPQ CPHST, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 163 EP 163 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800849 ER PT J AU Cilia, ML AF Cilia, M. L. TI Using proteomics and mass spectrometry to explore the dynamic virus-vector interface SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Cilia, M. L.] ARS, USDA, Ithaca, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 164 EP 164 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800858 ER PT J AU Fisher, TW He, R Munyaneza, J Crosslin, J Brown, JK AF Fisher, T. W. He, R. Munyaneza, J. Crosslin, J. Brown, J. K. TI The effect of temperature on 'Candidatus Liberibacter solanacearum' gene expression SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Fisher, T. W.; Brown, J. K.] Univ Arizona, Tucson, AZ USA. [He, R.] Washington State Univ, Pullman, WA 99164 USA. [Munyaneza, J.] ARS, USDA, YARL, Wapato, WA USA. [Crosslin, J.] ARS, USDA, YARL, Prosser, WA USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 164 EP 164 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800855 ER PT J AU Gardner, CA AF Gardner, C. A. TI The National Plant Germplasm System (NPGS) and GRIN-Global SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Gardner, C. A.] ARS, USDA, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 166 EP 166 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800870 ER PT J AU Ellis, D AF Ellis, D. TI Plant germplasm curation-Best practices SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Annual Meeting of the American-Phytopathological-Society (APS) CY AUG 04-08, 2012 CL Providence, RI SP Amer Phytopathol Soc (APS) C1 [Ellis, D.] ARS, USDA, Natl Ctr Genet Resources Preservat, Ft Collins, CO USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2012 VL 102 IS 7 SU 4 BP 167 EP 167 PG 1 WC Plant Sciences SC Plant Sciences GA 196SX UT WOS:000322797800872 ER PT J AU Loper, JE Hassan, KA Mavrodi, DV Davis, EW Lim, CK Shaffer, BT Elbourne, LDH Stockwell, VO Hartney, SL Breakwell, K Henkels, MD Tetu, SG Rangel, LI Kidarsa, TA Wilson, NL de Mortel, JEV Song, CX Blumhagen, R Radune, D Hostetler, JB Brinkac, LM Durkin, AS Kluepfel, DA Wechter, WP Anderson, AJ Kim, YC Pierson, LS Pierson, EA Lindow, SE Kobayashi, DY Raaijmakers, JM Weller, DM Thomashow, LS Allen, AE Paulsen, IT AF Loper, Joyce E. Hassan, Karl A. Mavrodi, Dmitri V. Davis, Edward W., II Lim, Chee Kent Shaffer, Brenda T. Elbourne, Liam D. H. Stockwell, Virginia O. Hartney, Sierra L. Breakwell, Katy Henkels, Marcella D. Tetu, Sasha G. Rangel, Lorena I. Kidarsa, Teresa A. Wilson, Neil L. de Mortel, Judith E. van Song, Chunxu Blumhagen, Rachel Radune, Diana Hostetler, Jessica B. Brinkac, Lauren M. Durkin, A. Scott Kluepfel, Daniel A. Wechter, W. Patrick Anderson, Anne J. Kim, Young Cheol Pierson, Leland S., III Pierson, Elizabeth A. Lindow, Steven E. Kobayashi, Donald Y. Raaijmakers, Jos M. Weller, David M. Thomashow, Linda S. Allen, Andrew E. Paulsen, Ian T. TI Comparative Genomics of Plant-Associated Pseudomonas spp.: Insights into Diversity and Inheritance of Traits Involved in Multitrophic Interactions SO PLOS GENETICS LA English DT Article ID III SECRETION SYSTEM; SYRINGAE PV. SYRINGAE; GAMMA-AMINOBUTYRIC-ACID; BIOLOGICAL-CONTROL; FLUORESCENS PF-5; BIOCONTROL STRAIN; ANTIBIOTIC PRODUCTION; SECONDARY METABOLITE; PHENYLACETIC ACID; CHLORORAPHIS O6 AB We provide here a comparative genome analysis of ten strains within the Pseudomonas fluorescens group including seven new genomic sequences. These strains exhibit a diverse spectrum of traits involved in biological control and other multitrophic interactions with plants, microbes, and insects. Multilocus sequence analysis placed the strains in three sub-clades, which was reinforced by high levels of synteny, size of core genomes, and relatedness of orthologous genes between strains within a sub-clade. The heterogeneity of the P. fluorescens group was reflected in the large size of its pan-genome, which makes up approximately 54% of the pan-genome of the genus as a whole, and a core genome representing only 45-52% of the genome of any individual strain. We discovered genes for traits that were not known previously in the strains, including genes for the biosynthesis of the siderophores achromobactin and pseudomonine and the antibiotic 2-hexyl-5-propyl-alkylresorcinol; novel bacteriocins; type II, III, and VI secretion systems; and insect toxins. Certain gene clusters, such as those for two type III secretion systems, are present only in specific sub-clades, suggesting vertical inheritance. Almost all of the genes associated with multitrophic interactions map to genomic regions present in only a subset of the strains or unique to a specific strain. To explore the evolutionary origin of these genes, we mapped their distributions relative to the locations of mobile genetic elements and repetitive extragenic palindromic (REP) elements in each genome. The mobile genetic elements and many strain-specific genes fall into regions devoid of REP elements (i.e., REP deserts) and regions displaying atypical tri-nucleotide composition, possibly indicating relatively recent acquisition of these loci. Collectively, the results of this study highlight the enormous heterogeneity of the P. fluorescens group and the importance of the variable genome in tailoring individual strains to their specific lifestyles and functional repertoire. C1 [Loper, Joyce E.; Davis, Edward W., II; Shaffer, Brenda T.; Henkels, Marcella D.; Kidarsa, Teresa A.; Blumhagen, Rachel] ARS, USDA, Corvallis, OR USA. [Loper, Joyce E.; Stockwell, Virginia O.; Hartney, Sierra L.; Rangel, Lorena I.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Hassan, Karl A.; Lim, Chee Kent; Elbourne, Liam D. H.; Breakwell, Katy; Tetu, Sasha G.; Wilson, Neil L.; Paulsen, Ian T.] Macquarie Univ, Dept Chem & Biomol Sci, Sydney, NSW 2109, Australia. [Mavrodi, Dmitri V.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [de Mortel, Judith E. van; Song, Chunxu; Raaijmakers, Jos M.] Wageningen Univ, Phytopathol Lab, Wageningen, Netherlands. [Radune, Diana; Hostetler, Jessica B.; Brinkac, Lauren M.; Durkin, A. Scott] J Craig Venter Inst, Rockville, MD USA. [Kluepfel, Daniel A.] ARS, USDA, Davis, CA USA. [Wechter, W. Patrick] ARS, USDA, Charleston, SC USA. [Anderson, Anne J.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Kim, Young Cheol] Chonnam Natl Univ, Inst Environmentally Friendly Agr, Kwangju, South Korea. [Pierson, Leland S., III] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Pierson, Elizabeth A.] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. [Lindow, Steven E.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Kobayashi, Donald Y.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08903 USA. [Weller, David M.; Thomashow, Linda S.] ARS, USDA, Pullman, WA USA. [Allen, Andrew E.] J Craig Venter Inst, San Diego, CA USA. RP Loper, JE (reprint author), ARS, USDA, Corvallis, OR USA. EM Joyce.Loper@ars.usda.gov RI Paulsen, Ian/K-3832-2012; Hassan, Karl/B-2802-2013; Tetu, Sasha/C-8760-2013; Mavrodi, Dmitri/J-2745-2013; Anderson, Anne/B-7313-2014; Raaijmakers, Jos/D-1574-2014; Song, Chunxu/D-1719-2014; Elbourne, Liam/G-6150-2011; OI Hassan, Karl/0000-0003-2031-9679; Paulsen, Ian/0000-0001-9015-9418; Raaijmakers, Jos/0000-0003-1608-6614; Song, Chunxu/0000-0002-9860-5395; Elbourne, Liam/0000-0002-9784-0959; Loper, Joyce/0000-0003-3501-5969 FU USDA National Institute of Food and Agriculture [2008-35600-18770]; Australian Research Council [DP110102680] FX This project was supported by the Agriculture and Food Research Initiative Competitive Grants Program Grant no. 2008-35600-18770 from the USDA National Institute of Food and Agriculture. A portion of this work was funded by grant DP110102680 from the Australian Research Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 178 TC 157 Z9 160 U1 11 U2 95 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD JUL PY 2012 VL 8 IS 7 AR e1002784 DI 10.1371/journal.pgen.1002784 PG 27 WC Genetics & Heredity SC Genetics & Heredity GA 979SP UT WOS:000306840400011 PM 22792073 ER PT J AU Ng, CS Wu, P Foley, J Foley, A McDonald, ML Juan, WT Huang, CJ Lai, YT Lo, WS Chen, CF Leal, SM Zhang, HM Widelitz, RB Patel, PI Li, WH Chuong, CM AF Ng, Chen Siang Wu, Ping Foley, John Foley, Anne McDonald, Merry-Lynn Juan, Wen-Tau Huang, Chih-Jen Lai, Yu-Ting Lo, Wen-Sui Chen, Chih-Feng Leal, Suzanne M. Zhang, Huanmin Widelitz, Randall B. Patel, Pragna I. Li, Wen-Hsiung Chuong, Cheng-Ming TI The Chicken Frizzle Feather Is Due to an alpha-Keratin (KRT75) Mutation That Causes a Defective Rachis SO PLOS GENETICS LA English DT Article ID HAIR FOLLICLE; EVOLUTIONARY ORIGIN; COMPANION LAYER; MODEL ORGANISM; GENE FAMILY; EVO-DEVO; INTERMEDIATE-FILAMENTS; VERTEBRATE EVOLUTION; GENOMIC RESOURCES; SKIN APPENDAGES AB Feathers have complex forms and are an excellent model to study the development and evolution of morphologies. Existing chicken feather mutants are especially useful for identifying genetic determinants of feather formation. This study focused on the gene F, underlying the frizzle feather trait that has a characteristic curled feather rachis and barbs in domestic chickens. Our developmental biology studies identified defects in feather medulla formation, and physical studies revealed that the frizzle feather curls in a stepwise manner. The frizzle gene is transmitted in an autosomal incomplete dominant mode. A whole-genome linkage scan of five pedigrees with 2678 SNPs revealed association of the frizzle locus with a keratin gene-enriched region within the linkage group E22C19W28_E50C23. Sequence analyses of the keratin gene cluster identified a 69 bp in-frame deletion in a conserved region of KRT75, an a-keratin gene. Retroviral-mediated expression of the mutated F cDNA in the wild-type rectrix qualitatively changed the bending of the rachis with some features of frizzle feathers including irregular kinks, severe bending near their distal ends, and substantially higher variations among samples in comparison to normal feathers. These results confirmed KRT75 as the F gene. This study demonstrates the potential of our approach for identifying genetic determinants of feather forms. C1 [Ng, Chen Siang; Huang, Chih-Jen; Lai, Yu-Ting; Lo, Wen-Sui; Li, Wen-Hsiung] Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan. [Wu, Ping; Widelitz, Randall B.; Chuong, Cheng-Ming] Univ So Calif, Keck Sch Med, Dept Pathol, Los Angeles, CA 90033 USA. [Foley, John; Foley, Anne] Indiana Univ Sch Med, Dept Anat & Cell Biol, Bloomington, IN USA. [Foley, John; Foley, Anne; Leal, Suzanne M.] Indiana Univ Sch Med, Dept Dermatol, Bloomington, IN USA. [McDonald, Merry-Lynn] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Juan, Wen-Tau] Acad Sinica, Inst Phys, Taipei, Taiwan. [Huang, Chih-Jen] Acad Sinica, Taiwan Int Grad Program, Taipei 115, Taiwan. [Huang, Chih-Jen] Natl Chung Hsing Univ, Grad Inst Biotechnol, Taichung 40227, Taiwan. [Chen, Chih-Feng] Natl Chung Hsing Univ, Dept Anim Sci, Taichung 40227, Taiwan. [Zhang, Huanmin] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI USA. [Patel, Pragna I.] Univ So Calif, Inst Med Genet, Los Angeles, CA USA. [Patel, Pragna I.] Univ So Calif, Ctr Craniofacial Mol Biol, Los Angeles, CA 90033 USA. [Li, Wen-Hsiung] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA. [Chuong, Cheng-Ming] Natl Taiwan Univ, Res Ctr Dev Biol & Regenerat Med, Taipei 10764, Taiwan. RP Ng, CS (reprint author), Acad Sinica, Biodivers Res Ctr, Taipei 115, Taiwan. EM whli@gate.sinica.edu.tw; cmchuong@usc.edu FU National Science Council of Taiwan [99-2321-B-001-041-MY2]; National Institutes of Health of USA [AR 47364]; Academia Sinica Research Program on Nanoscience and Nanotechnology; Academia Sinica of Taiwan FX W-HL acknowledges the National Science Council of Taiwan grant number 99-2321-B-001-041-MY2. C-MC acknowledges the National Institutes of Health of USA grant number AR 47364. W-TJ acknowledges the funding support from Academia Sinica Research Program on Nanoscience and Nanotechnology. CSN acknowledges the postdoctoral fellowship from Academia Sinica of Taiwan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 98 TC 29 Z9 29 U1 10 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD JUL PY 2012 VL 8 IS 7 AR e1002748 DI 10.1371/journal.pgen.1002748 PG 16 WC Genetics & Heredity SC Genetics & Heredity GA 979SP UT WOS:000306840400005 PM 22829773 ER PT J AU Haynes, J Cubbage, F Mercer, E Sills, E AF Haynes, Jessica Cubbage, Frederick Mercer, Evan Sills, Erin TI The Search for Value and Meaning in the Cocoa Supply Chain in Costa Rica SO SUSTAINABILITY LA English DT Article DE cocoa; supply chain; certification; trade; marketing; integration of meaning; Costa Rica AB Qualitative interviews with participants in the cocoa (Theobroma cacao) supply chain in Costa Rica and the United States were conducted and supplemented with an analysis of the marketing literature to examine the prospects of organic and Fairtrade certification for enhancing environmentally and socially responsible trade of cocoa from Costa Rica. Respondents were familiar with both systems, and most had traded at least organic cocoa for some period. However, most individuals said that they were seeking better product differentiation and marketing than has been achieved under the organic and Fairtrade systems. Many suggested that more direct recognition of individual growers and the unique value of their cocoa throughout the production chain would be more helpful than certification for small companies in the cocoa supply chain. These findings suggest new marketing techniques that convey an integration of meaning into the cocoa and chocolate supply chain as a differentiation strategy. This involves integration of the story of producers' commitment and dedication; shared producer and consumer values of social and environmental responsibility; and personal relationships between producers and consumers. This marketing approach could enhance the ability of smaller companies to successfully vie with their larger competitors and to produce cocoa in a more environmentally and socially acceptable manner. C1 [Haynes, Jessica; Cubbage, Frederick; Sills, Erin] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Mercer, Evan] USDA Forest Serv, Southern Res Stn, Res Triangle Pk, NC 27709 USA. RP Cubbage, F (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Box 8008, Raleigh, NC 27695 USA. EM jessica.gertrude@gmail.com; fredcubbage@yahoo.com; emercer@fs.fed.us; erin_sills@ncsu.edu FU U.S. AID; USDA Forest Service; North Carolina State University FX Partial funding for this research was provided by U.S. AID and the USDA Forest Service, as well as North Carolina State University. Ruth Junkins, Eduardo Sommarriba, and Marilyn Villalobos at CATIE assisted with guidance about cocoa in Costa Rica, survey procedures, and contacts for participants in the cocoa supply chain. The persons interviewed provided profound and candid comments about the cocoa supply chain strengths and weaknesses. The external reviewers also provided very helpful, constructive suggestions. Responsibility for errors and oversights of course rests with the authors. NR 34 TC 4 Z9 5 U1 5 U2 36 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD JUL PY 2012 VL 4 IS 7 BP 1466 EP 1487 DI 10.3390/su4071466 PG 22 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA 213FT UT WOS:000324041200006 ER PT J AU Reinhart, KO Wilson, GWT Rinella, MJ AF Reinhart, Kurt O. Wilson, Gail W. T. Rinella, Matthew J. TI Predicting plant responses to mycorrhizae: integrating evolutionary history and plant traits SO ECOLOGY LETTERS LA English DT Article DE Arbuscular mycorrhizal fungi; evolution; grassland; mycorrhizal responsiveness; phylogenetic relatedness; phylogenetic signal; root colonization ID PHYLOGENETIC SIGNAL; TALLGRASS PRAIRIE; LAND PLANTS; FUNGI; DIVERSITY; GRASSES; FIELD; DEPENDENCY; LIMITATION AB We assessed whether (1) arbuscular mycorrhizal colonization of roots (RC) and/or plant responses to arbuscular mycorrhizae (MR) vary with plant phylogeny and (2) MR and RC can be more accurately predicted with a phylogenetic predictor relative to a null model and models with plant trait and taxonomic predictors. In a previous study, MR and RC of 95 grassland species were measured. We constructed a phylogeny for these species and found it explained variation in MR and RC. Next, we used multiple regressions to identify the models that most accurately predicted plant MR. Models including either phylogenetic or phenotypic and taxonomic information similarly improved our ability to predict MR relative to a null model. Our study illustrates the complex evolutionary associations among species and constraints of using phylogenetic information, relative to plant traits, to predict how a plant species will interact with AMF. C1 [Reinhart, Kurt O.; Rinella, Matthew J.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Wilson, Gail W. T.] Oklahoma State Univ, Nat Resource Ecol & Management, Stillwater, OK 74078 USA. RP Reinhart, KO (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM kurt.reinhart@ars.usda.gov RI Wilson, Gail/G-4255-2012; Reinhart, Kurt/A-6730-2010 NR 48 TC 51 Z9 52 U1 5 U2 117 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD JUL PY 2012 VL 15 IS 7 BP 689 EP 695 DI 10.1111/j.1461-0248.2012.01786.x PG 7 WC Ecology SC Environmental Sciences & Ecology GA 955EN UT WOS:000305000000007 PM 22507627 ER PT J AU Zhang, HY Wang, H Guo, SG Ren, Y Gong, GY Weng, YQ Xu, Y AF Zhang, Haiying Wang, Hui Guo, Shaogui Ren, Yi Gong, Guoyi Weng, Yiqun Xu, Yong TI Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai SO EUPHYTICA LA English DT Article DE Citrullus lanatus; Fingerprinting; Genetic diversity; Microsatellites; Watermelon ID VEGETABLE CULTIVAR DESCRIPTIONS; NEIGHBOR-JOINING METHOD; SIMPLE SEQUENCE REPEATS; PHYLOGENETIC TREES; NORTH-AMERICA; SSR MARKERS; AFLP; GENOME; RAPD; COLLECTION AB Watermelon, Citrullus lanatus Thunb. Matsum. & Nakai is an important vegetable crop worldwide. Due to its narrow genetic base, detection and utilization of the genetic variations, cultivar identification and increasing genetic diversity are some important tasks for watermelon breeders. Molecular markers, especially microsatellites or simple sequence repeats (SSRs) are playing increasingly important roles for these purposes. In the present study, a core set of 23 highly informative SSR markers was developed for watermelon genetic diversity analysis. Based on whole genome sequencing of 17 watermelon inbred lines, we identified 3.9 million single nucleotide polymorphisms (SNPs) which were used to construct a SNP-based dendrogram for the 17 lines. Meanwhile, from the sequenced genome, 13,744 SSRs were developed, of which 704 were placed on a high-resolution watermelon linkage map. To develop the core set SSR markers, 78 of the 704 mapped SSRs were selected as the candidate markers. Using the SNP-based dendrogram as calibration, 23 SSR markers evenly distributed across the genome were identified as the core marker set for watermelon genetic diversity analysis. Each marker was able to detect 2-7 alleles with polymorphism information content values ranging from 0.45 to 0.82. The dendrograms of 17 watermelon lines based on SNPs, the base set of 78 SSRs and the core set of 23 SSRs were highly consistent. The utility of this core set SSRs was demonstrated in 100 commercial watermelon cultivars and elite lines, which could be placed into six clusters that were largely consistent with previous classification based on morphology and parentage data. This core set of SSR markers should be very useful for genotyping and genetic variation analysis in watermelon. C1 [Zhang, Haiying; Wang, Hui; Guo, Shaogui; Ren, Yi; Gong, Guoyi; Xu, Yong] Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. [Weng, Yiqun] Univ Wisconsin, ARS, USDA, Vegetable Crops Res Unit,Hort Dept, Madison, WI 53719 USA. RP Xu, Y (reprint author), Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China. EM xuyong@nercv.org FU Beijing Municipal Science & Technology Commission, China [D08070500690803, D111100001311002, KJCX201101010, Z09090501040902, 5100001]; Ministry of Science and Technology of China [2009BADB8B02, 2010DFB33740, 30972015, 31171980, 2010AA10A107]; Ministry of Agriculture of China [CARS-26] FX This work was supported by funds from Beijing Municipal Science & Technology Commission, China (D08070500690803, D111100001311002, KJCX201101010, Z09090501040902 and 5100001); The Ministry of Science and Technology of China (2009BADB8B02, 2010DFB33740, 30972015, 31171980 and 2010AA10A107), and the Ministry of Agriculture of China (CARS-26). NR 53 TC 13 Z9 18 U1 0 U2 32 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 2 BP 329 EP 342 DI 10.1007/s10681-011-0574-z PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 951CK UT WOS:000304698100006 ER PT J AU Liang, ZC Sang, M Wu, BH Ma, AH Zhao, SJ Zhong, GY Li, SH AF Liang, Zhenchang Sang, Min Wu, Benhong Ma, Aihong Zhao, Shengjian Zhong, Gan-Yuan Li, Shaohua TI Inheritance of anthocyanin content in the ripe berries of a tetraploid x diploid grape cross population SO EUPHYTICA LA English DT Article DE Anthocyanins; Inheritance; Transgressive segregation; Polyploid table grapes ID VITIS-VINIFERA L; ROTUNDIFOLIA MICHX; WINE COLOR; QUALITY; HYBRID; SKIN AB Variation patterns and inheritance of anthocyanin content in the ripe berries of a tetraploid x diploid table grape cross population were investigated in two successive years. The population segregated for three different ploid levels: dipolids, triploids, and tetraploids. A total of 28 different anthocyanins were detected and quantified in the progeny population. Transgressive segregation for the total anthocyanin content was observed in all the three ploid progeny populations. The total anthocyanin content increased as the ploid level increased. The broad sense heritabilities (H-2) of the total anthocyanin content were all relatively high, ranging from 0.53 to 0.98, 0.57 to 0.97 and 0.43 to 0.94 in the diploid, triploid and tetraploid population, respectively. Our results suggested that the total anthocyanin content followed an additive inheritance model in this polyploid segregation population. We also observed that the relative contribution of individual anthocyanins to the total anthocyanin content varied significantly among different ploid populations, suggesting that genetic background has important impact on the accumulation of the individual anthocyanin compounds. These results will help develop better breeding strategies in a polyploid table grape breeding program for improving the content of anthocyanins, an important class of polyphenolics possessing antioxidant activities and many other health-related benefits. C1 [Liang, Zhenchang; Li, Shaohua] Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China. [Liang, Zhenchang; Wu, Benhong] Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Wine Technol, Beijing 100093, Peoples R China. [Liang, Zhenchang; Wu, Benhong] Chinese Acad Sci, Inst Bot, Key Lab Plant Resource Sci, Beijing 100093, Peoples R China. [Sang, Min] Jinan Univ, Inst Hydrobiol, Guangzhou 510632, Guangdong, Peoples R China. [Ma, Aihong; Zhao, Shengjian] Hebei Acad Agr & Forestry, Inst Fruits Changli, Shihchiachuang 066600, Hebei, Peoples R China. [Zhong, Gan-Yuan] ARS, USDA, Grape Genet Res Unit, Geneva, NY 14456 USA. RP Li, SH (reprint author), Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan Bot Garden, Wuhan 430074, Peoples R China. EM shhli@wbgcas.cn RI Wu, Benhong/Q-5328-2016 FU China 973 project [2011CB100605]; National Natural Science Foundation of China [31071757]; CAS/CAFEA [0921101001] FX This work was supported by China 973 project [2011CB100605], the National Natural Science Foundation of China (31071757) and CAS/CAFEA Intl. Partnership Program for Creative Research Teams (0921101001). NR 34 TC 4 Z9 4 U1 4 U2 39 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 2 BP 343 EP 356 DI 10.1007/s10681-011-0594-8 PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 951CK UT WOS:000304698100007 ER PT J AU Prohens, J Plazas, M Raigon, MD Segui-Simarro, JM Stommel, JR Vilanova, S AF Prohens, Jaime Plazas, Mariola Raigon, Maria D. Segui-Simarro, Jose M. Stommel, John R. Vilanova, Santiago TI Characterization of interspecific hybrids and first backcross generations from crosses between two cultivated eggplants (Solanum melongena and S-aethiopicum Kumba group) and implications for eggplant breeding SO EUPHYTICA LA English DT Article DE Backcrosses; Descriptors; Solanum melongena; Solanum aethiopicum; SSRs; Tomato analyzer ID FERTILE SOMATIC HYBRIDS; FRUIT SHAPE; MACROCARPON L; L.; TOMATO; RESISTANCE; SOLANACEAE; DIVERSITY; GILO; RECOMBINATION AB Common (Solanum melongena L.) and scarlet (S. aethiopicum L.) eggplants are cultivated for their fruits and form part of the same genepool. We have studied plant and fruit characteristics, pollen viability and seed set, phenolics content, and fruit flesh browning in accessions of S. melongena and S. aethiopicum Kumba group, as well as interspecific hybrids between these species and first backcross generations to each parental species. Respective genotypes were also characterized with seven polymorphic SSR markers. The results demonstrate that many differences exist for plant and fruit morphology among S. melongena, S. aethiopicum and the interspecific hybrids. The latter are very vigorous and generally intermediate between the two parents, except for fruit size which is smaller (and parthenocarpic due to a high pollen sterility) than those of any of the parents. Backcross progenies also exhibited morphological variation with moderate heritability values for the attributes evaluated. Variation for fruit size was present in the backcross generations but fruits were small resulting in little variation for fruit shape. Backcross plants with moderate fertility produced seeded fruits. Primary hybrids had fruit phenolics content similar to that of S. aethiopicum, the parent with lowest phenolics concentration, and were heterotic for fruit flesh browning. Backcross progenies were quite variable for both traits. SSR markers did not reveal segregation distortion in the backcross generations for these interspecific hybrids. The results demonstrate that generations derived from sexual interspecific hybridization can be a powerful tool for S. melongena and S. aethiopicum Kumba group breeding. C1 [Prohens, Jaime; Plazas, Mariola; Segui-Simarro, Jose M.; Vilanova, Santiago] Univ Politecn Valencia, Inst Conservac & Mejora Agrodiversidad Valenciana, Valencia 46022, Spain. [Raigon, Maria D.] Univ Politecn Valencia, Dept Quim, Valencia 46022, Spain. [Stommel, John R.] ARS, USDA, Ctr Plant Sci Inst, Genet Improvement Fruits & Vegetables Lab, Beltsville, MD 20705 USA. RP Prohens, J (reprint author), Univ Politecn Valencia, Inst Conservac & Mejora Agrodiversidad Valenciana, Camino de Vera 14, Valencia 46022, Spain. EM jprohens@btc.upv.es RI Segui-Simarro, Jose M./J-5959-2014; Prohens, Jaime/H-2228-2015 OI Segui-Simarro, Jose M./0000-0001-7672-4169; Prohens, Jaime/0000-0003-1181-9065 FU Ministerio de Ciencia y Tecnologia [AGL2009-07257, RF-2008-00008-00-00] FX This work was partially financed by the Ministerio de Ciencia y Tecnologia (AGL2009-07257 and RF-2008-00008-00-00). NR 57 TC 16 Z9 17 U1 2 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 2 BP 517 EP 538 DI 10.1007/s10681-012-0652-x PG 22 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 951CK UT WOS:000304698100021 ER PT J AU Lewers, KS Luo, YG Vinyard, BT AF Lewers, Kim S. Luo, Yaguang Vinyard, Bryan T. TI Evaluating strawberry breeding selections for postharvest fruit decay SO EUPHYTICA LA English DT Article DE Selection methods; Fragaria; Fruit quality; Botrytis; Fruit rot ID ROT; RESISTANCE; CULTIVARS; CLONES AB Fruit from the annual replicated yield assessments for the USDA-ARS strawberry (Fragaria xananassa Duchesne ex Rozier) breeding program at Beltsville, MD, 2007 and 2008, were evaluated for postharvest decay development after storage at 5 A degrees C. Generalized linear genotype x year mixed model analysis of covariance with overdispersed binomial distribution, logit link function, and Laplace optimization method was used to determine the relationships between postharvest proportion decay and storage period (SP), season's harvest number (HN), and genotype-specific harvest number through the season; conditioned on the confounding of harvest with SP. To determine if limiting analyses to replicates (plot x harvest combinations) with a minimum number of berries would increase statistical precision, concurrent analyses were conducted using data from replicates with more than: zero, three, and six berries. Limiting data to genotypes for which more than three or more than six berries could be evaluated, resulted in loss of data and reduced statistical power. Within the range of SP observed, 13-19 days, consistency among genotypes of the linear relationship between SP and logit postharvest proportion decay, unless strongly influenced by a confounded harvest effect, indicated all SP in the range tested provided equivalent ability to discern genotypes. The significant genotype x HN interaction effect indicated maximizing the number of harvests was preferable to limiting to a few "informative" harvests. The significant genotype x year interaction effect indicated the decision to release a genotype as a cultivar will demand multiple years of evaluation. Making determinations each year to discard genotypes or use them as parents for postharvest quality was best facilitated by the simple process of calculating arithmetic genotypic means for each harvest date separately, charting the results, and using the charts to visually assess genotypic performance trends across harvests. C1 [Lewers, Kim S.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Luo, Yaguang] ARS, Food Qual Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Vinyard, Bryan T.] ARS, Biometr Consulting Serv, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Lewers, KS (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville Agr Res Ctr, Bldg 010A,10300 Baltimore Ave,BARC W, Beltsville, MD 20705 USA. EM Kim.Lewers@ARS.USDA.gov FU USDA-ARS [1275-21220-189-00, 1265-42000-004-00] FX This project was funded by USDA-ARS Projects 1275-21220-189-00 and 1265-42000-004-00. The authors wish to thank Jim Ballington for donation of breeding selections; John Enns, Phil Edmonds, and the BARC Research Support Services for establishing and maintaining the fields; Ellen Turner, John Enns, and Qiang He for evaluating fruit; George Meyers, RSS, for weather records; and Wayne Jurick, Gene Lester, John Maas, Lisa J. Rowland, and the anonymous reviewers for their helpful comments. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 18 TC 2 Z9 2 U1 2 U2 31 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 2 BP 539 EP 555 DI 10.1007/s10681-012-0654-8 PG 17 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 951CK UT WOS:000304698100022 ER PT J AU Haynes, KG Gergela, DM Hutchinson, CM Yencho, GC Clough, ME Henninger, MR Halseth, DE Sandsted, E Porter, GA Ocaya, PC AF Haynes, K. G. Gergela, D. M. Hutchinson, C. M. Yencho, G. C. Clough, M. E. Henninger, M. R. Halseth, D. E. Sandsted, E. Porter, G. A. Ocaya, P. C. TI Early generation selection at multiple locations may identify potato parents that produce more widely adapted progeny SO EUPHYTICA LA English DT Article DE Potato; Solanum tuberosum; Genotype x environment interaction; Broad adaptation; Superior parents ID BREEDING PROGRAM; VISUAL SELECTION; CLONAL GENERATION; YIELD COMPONENTS; EFFICIENCY; RESISTANCE; CHARACTERS; SEEDLINGS AB Potato production in the eastern United States occurs over a wide range of environments from Maine to Florida; many of these states do not have a breeding program. Initial selections are usually made at the breeding location for more than 2 years before undergoing evaluation elsewhere, at which point, few clones remain from the original population. The purpose of this study was to determine if parents that produce progeny with broad adaptation could be identified early in the USDA/ARS Potato Breeding Program. For 3 years (2007-2009), seed of all second field generation clones was distributed to five locations for selection. In 2008, 2009 and 2010, selections were made among 339, 321, and 381 clones, respectively. For each parent in each year, Chi-square values were calculated from the observed and expected number of progeny selected. Each year, parents that produced poorly- or broadly-adapted offspring were identified. Among the progeny selected at three or more locations, 27 to 70 % had not been selected by the breeding program. Early generation selection can be used to identify parents that produce broadly adapted progeny and may identify broadly adapted progeny before they are discarded by the breeding program. Because potatoes display such strong genotype x environment interactions, this selection approach may be particularly beneficial for selecting potato cultivars with broad adaptation. C1 [Haynes, K. G.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. [Gergela, D. M.; Hutchinson, C. M.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Yencho, G. C.; Clough, M. E.] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Henninger, M. R.] Rutgers State Univ, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [Halseth, D. E.; Sandsted, E.] Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. [Porter, G. A.; Ocaya, P. C.] Univ Maine, Orono, ME 04469 USA. RP Haynes, KG (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM kathleen.haynes@ars.usda.gov NR 27 TC 6 Z9 7 U1 0 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 2 BP 573 EP 583 DI 10.1007/s10681-012-0685-1 PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 951CK UT WOS:000304698100024 ER PT J AU Pan, H Zheng, ZF Hse, CY AF Pan, Hui Zheng, Zhifeng Hse, Chung Y. TI Microwave-assisted liquefaction of wood with polyhydric alcohols and its application in preparation of polyurethane (PU) foams SO EUROPEAN JOURNAL OF WOOD AND WOOD PRODUCTS LA English DT Article ID STARCH; LIGNIN; POLYOL AB Microwave radiation was used as the heating source in southern pine wood liquefaction with PEG/glycerin binary solvent. It was found that microwave heating was more efficient than conventional oil bath heating for wood liquefaction. The wood residue content of the H2SO4 catalyzed liquefied wood dropped to zero within 5 min with microwave heating. The resulting liquefied wood polyols have suitable hydroxyl values for the preparation of rigid PU foams. Both the compressive strength and apparent modulus of the liquefied-wood-based PU foams increased as the isocyanate index increased from 80 to 120. The foams from H3PO4 catalyzed polyols had lower densities than those from H2SO4 and the petroleum-based controls. They also showed lower strength and modulus than those from H2SO4. Liquefied-wood-based PU foams generally have lower compressive strength and apparent modulus than the petroleum-based controls. However, they showed better restorability from deformation than the petroleum-based controls. C1 [Pan, Hui; Zheng, Zhifeng] Louisiana State Univ, Ctr Agr, Dean Lee Res Stn, Alexandria, LA 71302 USA. [Zheng, Zhifeng] SW Forestry Univ, Fac Wood Sci & Technol, Kunming 650224, Yunnan, Peoples R China. [Hse, Chung Y.] US Forest Serv, So Res Stn, USDA, Pineville, LA 71360 USA. RP Pan, H (reprint author), Louisiana State Univ, Ctr Agr, Dean Lee Res Stn, Alexandria, LA 71302 USA. EM Hpan@agcenter.lsu.edu NR 36 TC 16 Z9 21 U1 2 U2 49 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0018-3768 J9 EUR J WOOD WOOD PROD JI Eur. J. Wood Wood Prod. PD JUL PY 2012 VL 70 IS 4 BP 461 EP 470 DI 10.1007/s00107-011-0567-6 PG 10 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA 950ML UT WOS:000304653300009 ER PT J AU Jenkins, MJ Page, WG Hebertson, EG Alexander, ME AF Jenkins, Michael J. Page, Wesley G. Hebertson, Elizabeth G. Alexander, Martin E. TI Fuels and fire behavior dynamics in bark beetle-attacked forests in Western North America and implications for fire management SO FOREST ECOLOGY AND MANAGEMENT LA English DT Review DE Crown fire; Bark beetles; Fire behavior; Fire modeling; Fire suppression; Forest flammability ID MOUNTAIN PINE-BEETLE; SUB-ALPINE FORESTS; SPREADING SURFACE FIRE; ACTIVE CROWN FIRE; LODGEPOLE PINE; DISTURBANCE INTERACTIONS; ROCKY-MOUNTAINS; TREE MORTALITY; NATIONAL-PARK; WILDFIRE AB Declining forest health attributed to associations between extensive bark beetle-caused tree mortality, accumulations of hazardous fuels, wildfire, and climate change have catalyzed changes in forest health and wildfire protection policies of land management agencies. These changes subsequently prompted research to investigate the extent to which bark beetle-altered fuel complexes affect fire behavior. Although not yet rigorously quantified, the results of the investigations, in addition to a growing body of operational experience, indicate that predictable changes in surface, ladder and canopy fuel characteristics do occur over the course of a bark beetle rotation. Input of these changes in fuel characteristics into conventional fire behavior modeling systems can readily provide predictions of potential fire behavior, including the likelihood of crowning. However, several factors limit the direct application of these modeling systems in their current form and consequently, they may largely under predict fire potential in such stands. This presents a concern where extreme fire behavior involving both crowning and spotting coupled with flammable fuel conditions can pose serious challenges to incident management and threaten the safety of firefighters and the general public alike. In this paper, we review the nature and characteristics of bark beetle-altered fuel complexes in the conifer forests of the Interior West and the challenges of understanding the effects on extreme fire behavior, including the initiation and spread of crown fires. We also discuss how emerging fire management plans in the U.S. have begun to integrate wildfire management and other forest health objectives with the specific goal of achieving biodiversity and ecosystem resiliency while simultaneously reducing the existence of hazardous fuel complexes. (C) 2012 Elsevier B.V. All rights reserved. C1 [Jenkins, Michael J.; Page, Wesley G.; Alexander, Martin E.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Hebertson, Elizabeth G.] USDA Forest Serv, Ogden, UT USA. [Alexander, Martin E.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2H1, Canada. [Alexander, Martin E.] Univ Alberta, Alberta Sch Forest Sci & Management, Edmonton, AB T6G 2H1, Canada. RP Jenkins, MJ (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM mike.jenkins@usu.edu FU Utah Agricultural Experiment Station, Utah State University; USDA; Joint Fire Science Program [JFSP 11-1-4-16, JFSP 09-S-03-1] FX This research was supported by the Utah Agricultural Experiment Station, Utah State University and approved as journal paper number 8407. Funding for authors' work cited in this paper was provided by USDA Forest Service Evaluation Monitoring and Special Technology and Development Programs. This article is a contribution of Joint Fire Science Program projects JFSP 11-1-4-16 and JFSP 09-S-03-1. Thanks to M.G. Cruz, W. Lindquist, C.W. McHugh, M. Pritchard and Si. Pyne for their assistance in the preparation of this article. NR 133 TC 43 Z9 44 U1 5 U2 77 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2012 VL 275 BP 23 EP 34 DI 10.1016/j.foreco.2012.02.036 PG 12 WC Forestry SC Forestry GA 953UH UT WOS:000304898200003 ER PT J AU Zielinski, WJ Dunk, JR Gray, AN AF Zielinski, William J. Dunk, Jeffrey R. Gray, Andrew N. TI Estimating habitat value using forest inventory data: The fisher (Martes pennanti) in northwestern California SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fisher; Martes pennanti; Monitoring; Inventory; Habitat; Models ID OREGON FORESTS; MODELS; SUITABILITY; SELECTION; RANGE; CONSERVATION; MANAGEMENT AB Managing forests for multiple objectives requires balancing timber and vegetation management objectives with needs of sensitive species. Especially challenging is how to retain the habitat elements for species that are typically associated with late-seral forests. We develop a regionally specific, multivariate model describing habitat selection that can be used - when linked to an institutional forest inventory program - to assess, monitor and forecast habitat conditions for a key wildlife species. We use the fisher (Martes pennanti) in northwestern California as our example and develop a predictive model for resting habitat that is created using data from the specific region where it will be used. We explore how this resting habitat model differs from a similar model developed for the Sierra Nevada and consider the implications for forest management. We developed the model using MaxEnt by comparing vegetation data at 99 randomly selected fisher resting structures on public and tribal lands in northwestern California with 883 Forest Inventory and Analysis (FIA) plots within the same ecoregion. A total of 58 alternative vegetation models were specified and the top 10 were nearly identical in their performance (Gain > 1.08; Area Under the Curve [AUC] > 0.89). We chose a five-variable model (canopy closure, tree age, total basal area, volume of "large" wood and basal area of hardwoods) because it included the fewest variables and included only those that could be affected by management. This model was similar to the Sierra Nevada model, but did not include topographic features (e.g., slope) nor did it include a variable representing the density of small trees. The absence of variables related to topography may make it easier for managers to affect positive change in resting habitat suitability since all variables can be influenced by management actions. Moreover, the model indicates that small trees appear to be less important (compared to southern Sierra Nevada) and therefore the probability of producing high-value resting habitat without higher fire risk is greater. We also created a spreadsheet that simplifies the process of generating habitat predictions from new data. Since metrics of stand structure and wildlife habitat are sensitive to sample design, collecting new data with FIA protocols will provide the most accurate estimates of predicted habitat with this model. Together, the Sierra Nevada and northwest California models provide managers in California a quantitative means to assess and monitor resting habitat suitability using current and future data that are part of an institutionally supported program to inventory forest vegetation. Published by Elsevier B.V. C1 [Zielinski, William J.] USDA Forest Serv, Pacific SW Res Stn, Arcata, CA 95521 USA. [Dunk, Jeffrey R.] Humboldt State Univ, Dept Environm Sci & Management, Arcata, CA 95521 USA. [Gray, Andrew N.] USDA Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Zielinski, WJ (reprint author), USDA Forest Serv, Pacific SW Res Stn, Arcata, CA 95521 USA. EM bzielinski@fs.fed.us FU USDA Forest Service Pacific Southwest Research Station; US Fish and Wildlife Service office in Yreka, California; Six Rivers National Forest FX We thank the USDA Forest Service Pacific Southwest Research Station, the US Fish and Wildlife Service office in Yreka, California, and the Six Rivers National Forest for support and M. Highley and the Hoopa tribe for contributing data. R. Schlexer provided assistance in the field and Tom Gaman and East-West Forestry Associates collected the field FIA data. NR 37 TC 1 Z9 1 U1 4 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2012 VL 275 BP 35 EP 42 DI 10.1016/j.foreco.2012.03.008 PG 8 WC Forestry SC Forestry GA 953UH UT WOS:000304898200004 ER PT J AU Greenberg, CH Keyser, TL Zarnoch, SJ Connor, K Simon, DM Warburton, GS AF Greenberg, Cathryn H. Keyser, Tara L. Zarnoch, Stanley J. Connor, Kristina Simon, Dean M. Warburton, Gordon S. TI Acorn viability following prescribed fire in upland hardwood forests SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Acorns; Germination; Hard mast; Prescribed fire; Restoration; Viability ID EASTERN NORTH-AMERICA; MIXED-OAK FORESTS; VEGETATION DYNAMICS; RED OAK; GROWTH; REGENERATION; TEMPERATURE; RECRUITMENT; GERMINATION; SEEDLINGS AB Restoration of structure and function of mixed-oak (Quercus spp.) forests is a focal issue of forest land managers in the eastern United States due to widespread regeneration failure and poor overstory recruitment of oaks, particularly on productive sites. Prescribed fire is increasingly used as a tool in oak ecosystem restoration, with the goal of reducing competition, and creating light and seedbed conditions conducive to germination and growth of oak seedlings. Yet, oak seedling establishment is dependent on the presence of viable acorns, which may be vulnerable to prescribed fire. We assessed the effect of prescribed burning and fire temperature on the viability of white oak and northern red oak acorns placed on the leaf litter surface, in the duff, or in the mineral soil during five winter prescribed burns in southern Appalachian upland hardwood forests. Fire temperatures varied among acorn plots, ranging from <79 to <371 degrees C. After the burns, acorns were planted in trays with water-saturated vermiculite, exposed to 14 continuous hours of light daily, and maintained at 27 degrees C on germination beds in a greenhouse. After three weeks we recorded the proportion of acorns germinating and the proportion of germinants with shoots. Our study indicated that patchy, low-intensity dormant season prescribed fire in upland hardwood forests reduced viability of white oak and northern red oak acorns located on the leaf litter surface, but did not generally affect acorns in the duff or mineral soil. Germination rates of both northern red oak and white oak acorns on the leaf litter surface decreased with increasing fire temperature. Shoot production by northern red oak germinants from acorns on the leaf litter surface (and less so in the duff), also decreased with increasing fire temperature. Acorns of both species on the leaf litter surface burned at temperatures >= 204 degrees C showed high mortality levels, with mortality virtually 100% at temperatures >= 260 degrees C. Fall burns, especially after a heavy acorn crop, could result in high acorn mortality, potentially impacting oak regeneration from seedlings for many years given erratic acorn production patterns among years and species. Frequent burning that reduces litter and duff depth could compromise availability of 'safe sites' where acorns are insulated from high fire temperatures. When oak ecosystem restoration is a goal, land managers should consider the timing and size of acorn crops, as well as the forest floor condition when determining the timing and frequency of prescribed burning. (C) 2012 Elsevier B.V. All rights reserved. C1 [Greenberg, Cathryn H.; Keyser, Tara L.] Forest Serv, USDA, So Res Stn, Asheville, NC 28806 USA. [Zarnoch, Stanley J.] Forest Serv, USDA, So Res Stn, Asheville, NC 28804 USA. [Connor, Kristina] Forest Serv, USDA, So Res Stn, GW Andrews Forestry Sci Lab, Auburn, AL 36849 USA. [Simon, Dean M.] N Carolina Wildlife Resources Commiss, Lawndale, NC 28090 USA. [Warburton, Gordon S.] N Carolina Wildlife Resources Commiss, Marion, NC 28752 USA. RP Greenberg, CH (reprint author), Forest Serv, USDA, So Res Stn, 1577 Brevard Rd, Asheville, NC 28806 USA. EM kgreenberg@fs.fed.us NR 44 TC 8 Z9 9 U1 1 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2012 VL 275 BP 79 EP 86 DI 10.1016/j.foreco.2012.03.012 PG 8 WC Forestry SC Forestry GA 953UH UT WOS:000304898200009 ER PT J AU Saenz-Romero, C Rehfeldt, GE Duval, P Lindig-Cisneros, RA AF Saenz-Romero, Cuauhtemoc Rehfeldt, Gerald E. Duval, Pierre Lindig-Cisneros, Roberto A. TI Abies religiosa habitat prediction in climatic change scenarios and implications for monarch butterfly conservation in Mexico SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Danaus plexippus; Suitable climatic habitat; Random Forests classification tree; Assisted migration; Climate change impacts; Responses to climate ID ALTITUDINAL GENETIC-VARIATION; UNITED-STATES; GLOBAL-CHANGE; PINUS-PATULA; POPULATIONS; MIGRATION; FORESTS; IMPACTS; RESTORATION; MANAGEMENT AB Abies religiosa (HBK) Schl. & Cham. (oyamel fir) is distributed in conifer-dominated mountain forests at high altitudes along the Trans-Mexican Volcanic Belt. This fir is the preferred host for overwintering monarch butterfly (Danaus plexippus) migratory populations which habitually congregate within a few stands now located inside a Monarch Butterfly Biosphere Reserve. Our objectives were to predict and map the climatic niche for A. religiosa for contemporary and future (2030, 2060 and 2090) climates, suggest management strategies to accommodate climate changes, and discuss implications for conservation of monarch butterfly overwintering sites in Mexico. A bioclimate model predicting the presence or absence of A. religiosa was developed by using the Random Forests classification tree on forest inventory data. The model used six predictor variables and was driven primarily by the mean temperature of the warmest month, an interaction between summer precipitation to and winter temperatures, and the ratio of summer to annual precipitation. Projecting the contemporary climate niche into future climates provided by three General Circulation Models and two scenarios suggested that the area occupied by the niche should diminish rapidly over the course of the century: a decrease of 69.2% by the decade surrounding 2030, 87.6% for that surrounding 2060, and 96.5% for 2090. We discuss assisted migration of A. religiosa upwards in altitude by 275 m so that populations of 2030 would occupy the same climates as today. The projections also show that by the end of the century, suitable habitat for the monarch butterfly may no longer occur inside the Biosphere Reserve. We therefore discuss management options and associated research programs necessary for assuring perpetuation of future butterfly habitat. (C) 2012 Elsevier B.V. All rights reserved. C1 [Saenz-Romero, Cuauhtemoc] Univ Michoacana San Nicolas de Hidalgo IIAF UMSNH, Inst Invest Agr & Forestales, Tarimbaro 58880, Michoacan, Mexico. [Rehfeldt, Gerald E.] USDA Forest Serv, Forestry Sci Lab, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Duval, Pierre] Ressources Nat Canada, Ctr Foresterie Laurentides, Serv Canadien Forets, Quebec City, PQ G1V 4C7, Canada. [Lindig-Cisneros, Roberto A.] Univ Nacinal Autonoma Mexico CIECO UNAM, Ctr Invest Ecosistemas, Morelia 58190, Michoacan, Mexico. RP Saenz-Romero, C (reprint author), Univ Michoacana San Nicolas de Hidalgo IIAF UMSNH, Inst Invest Agr & Forestales, Km 9-5 Carretera Morelia Zinapecuaro, Tarimbaro 58880, Michoacan, Mexico. EM csaenzromero@gmail.com; jrehfeldt@gmail.com; Pierre.Duval@rncan-nrcan.gc.ca; rlindig@oikos.unam.mx RI Lindig-Cisneros, Roberto/J-7306-2012 FU Mexican Council of Science and Technology (CONACYT); State of Michoacan (Fondo Mixto CONACyT-Michoacan) [2009-127128]; University of Michoacan (CIC, UMSNH); Mexican Integral Program for Institutional Strengthening Fund [PIFI-2009] FX This paper is an undertaking of the Forest Genetic Resources Working Group/North American Forest Commission/Food and Agricultural Organization of the United Nations. Financial support to CSR was provided a Grants by a joint research fund between the Mexican Council of Science and Technology (CONACYT) and the State of Michoacan (Fondo Mixto CONACyT-Michoacan, Project 2009-127128), the Coordination for Scientific Research of the University of Michoacan (CIC, UMSNH), and the Mexican Integral Program for Institutional Strengthening Fund (PIFI-2009). We thank Miriam Vargas-Llamas, Rigoberto Palafox-Rivas and Octavio Magana-Torres, Mexican National Forestry Commission (CONAFOR) for providing unpublished Mexican forest inventory data: Nicholas Crookston (USDA-Forest Service, Moscow, Idaho) for technical support; Karen Oberhauser (University of Minnesota), Rosendo Caro-Gomez (MBBR), Arnulfo Blanco-Garcia (Michoacan State Ministry of Urbanization and Environment, SUMA), and Martin Arriaga-Perez (Forest Development Department of Municipality of Ciudad Hidalgo) for valuable comments about biology of Monarch butterfly and A. religiosa; Juan Manuel Ortega-Rodriguez, School of Biology, UMSNH, for his aid on Arc Map; and an anonymous reviewer who provided valuable criticism on the manuscript. NR 52 TC 25 Z9 26 U1 4 U2 86 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2012 VL 275 BP 98 EP 106 DI 10.1016/j.foreco.2012.03.004 PG 9 WC Forestry SC Forestry GA 953UH UT WOS:000304898200011 ER PT J AU Ulyshen, MD Horn, S Hanula, JL AF Ulyshen, Michael D. Horn, Scott Hanula, James L. TI Growth responses of mature loblolly pine to dead wood manipulations SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Biomass; Coastal plain; Dendrochronology; Productivity; Sustainability; Tree rings ID SPRUCE PICEA-ABIES; WHOLE-TREE; BIOMASS REMOVAL; SITKA SPRUCE; SOIL COMPACTION; EARLY THINNINGS; SOUTHERN PINE; UNITED-STATES; DOUGLAS-FIR; NORTH WALES AB Large-scale manipulations of dead wood in mature Pinus taeda L. stands in the southeastern United States included a major one-time input of logs (fivefold increase in log volume) created by felling trees onsite, annual removals of all dead wood above >= 10 cm in diameter and >= 60 cm in length, and a reference in which no manipulations took place. We returned over a decade later to determine how these treatments affected tree growth using increment cores. There were no significant differences in tree density, basal area or tree diameters among treatments at the time of sampling. Although tree growth was consistently higher in the log-input plots and lower in the removal plots, this was true even during the 5 year period before the experiment began. When growth data from this initial period were included in the model as a covariate, no differences in post-treatment tree growth were detected. It is possible that treatment effects will become apparent after more time has passed, however. Published by Elsevier B.V. C1 [Ulyshen, Michael D.] USDA Forest Serv, So Res Stn, Starkville, MS 39759 USA. [Horn, Scott; Hanula, James L.] USDA Forest Serv, So Res Stn, Athens, GA USA. RP Ulyshen, MD (reprint author), USDA Forest Serv, So Res Stn, Starkville, MS 39759 USA. EM mulyshen@fs.fed.us NR 50 TC 1 Z9 1 U1 1 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2012 VL 275 BP 107 EP 110 DI 10.1016/j.foreco.2012.03.007 PG 4 WC Forestry SC Forestry GA 953UH UT WOS:000304898200012 ER PT J AU Brooks, RT Nislow, KH Lowe, WH Wilson, MK King, DI AF Brooks, Robert T. Nislow, Keith H. Lowe, Winsor H. Wilson, Matthew K. King, David I. TI Forest succession and terrestrial-aquatic biodiversity in small forested watersheds: a review of principles, relationships and implications for management SO FORESTRY LA English DT Article ID NORTHEASTERN UNITED-STATES; SOUTHERN NEW-ENGLAND; GROWTH RIPARIAN FORESTS; MULTIPLE SPATIAL SCALES; NORTH-AMERICAN FORESTS; HEADWATER STREAMS; NEW-HAMPSHIRE; BROOK TROUT; CLEAR-CUT; LOGGING HISTORY AB Early-successional forest habitat (ESFH), characterized by dense, short-statured woody vegetation, abundant and diverse herbaceous vegetation and high productivity-to-biomass ratios, supports diverse and productive terrestrial faunal communities. In upland forested areas, small watersheds, encompassing ecologically interconnected terrestrial and freshwater habitats, are fundamental units of the landscape for managing and protecting terrestrial and aquatic biodiversity. However, little is known about how the occurrence or abundance of upland ESFHs affects linkages between aquatic and terrestrial species and communities in these small watersheds. It is likely that the presence of ESFH in small watersheds affects the magnitude and direction of aquatic-terrestrial linkages, which may, in turn, affect overall biodiversity. We conducted a literature review of current information on aquatic and terrestrial biodiversity in small watersheds as related to forest age and structure. While the review identifies some fundamental uncertainties and information gaps, there is no evidence of negative effects of the creation and maintenance of upland ESFH on aquatic and riparian diversity. Increased aquatic production, due to higher light and nutrients, and increased primary and secondary production in ESFHs have the potential to increase watershed biodiversity. The review underscores the need for systematic evaluation of these potential outcomes to inform the management community. C1 [Brooks, Robert T.; Nislow, Keith H.; King, David I.] Univ Massachusetts, US Forest Serv, No Res Stn, Amherst, MA 01003 USA. [Lowe, Winsor H.; Wilson, Matthew K.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Brooks, RT (reprint author), Univ Massachusetts, US Forest Serv, No Res Stn, 201 Holdsworth Hall, Amherst, MA 01003 USA. EM rtbrooks@fs.fed.us FU Northeastern States Research Cooperative for the study of 'Forest Succession and Terrestrial-Aquatic Biodiversity in Northern Forest Watersheds: Relationships, Causes, and Implications for Management' [08-DG-11242307-111] FX We are grateful for the financial support provided by the Northeastern States Research Cooperative for the study of 'Forest Succession and Terrestrial-Aquatic Biodiversity in Northern Forest Watersheds: Relationships, Causes, and Implications for Management' (08-DG-11242307-111). NR 137 TC 7 Z9 7 U1 6 U2 62 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X J9 FORESTRY JI Forestry PD JUL PY 2012 VL 85 IS 3 BP 315 EP 327 DI 10.1093/forestry/cps031 PG 13 WC Forestry SC Forestry GA 955PN UT WOS:000305033500003 ER PT J AU Haavik, LJ Jones, JS Galligan, LD Guldin, JM Stephen, FM AF Haavik, Laurel J. Jones, Joshua S. Galligan, Larry D. Guldin, James M. Stephen, Fred M. TI Oak decline and red oak borer outbreak: impact in upland oak-hickory forests of Arkansas, USA SO FORESTRY LA English DT Article ID ENAPHALODES-RUFULUS HALDEMAN; SPECIES COMPOSITION; NORTHERN ARKANSAS; UNITED-STATES; MORTALITY; OZARK; CERAMBYCIDAE; COLEOPTERA; GROWTH; PENNSYLVANIA AB Oak-hickory forests in the Ozark and Ouachita Mountains of Arkansas recently experienced an episode of oak mortality in concert with an outbreak of the red oak borer (Enaphalodes rufulus (Haldeman) (Coleoptera: Cerambycidae)). We utilized data from the Forest Inventory and Analysis (FIA) program of the USDA Forest Service to explore changes in percent red oak (Quercus subgenus Erythrobalanus) mortality as a function of standing trees, basal area and stem density during and after the recent borer outbreak throughout the state of Arkansas. Mean red oak mortality levels in Arkansas FIA oak-hickory plots that were sampled both during and after the borer outbreak increased from 19 +/- 3 to 34 +/- 4 per cent of standing red oaks, resulting in significantly reduced red oak basal area and stem density in these stands. Mean size of red oaks did not change significantly during this time period, implying that all size classes experienced mortality. After red oak borer populations subsided, oak-hickory survey plots experienced increases in red oak mortality levels during a drought year (2006) and after an ice storm (2009), which suggests that these stress events, in addition to prior red oak borer infestation, could have had some influence on tree mortality. C1 [Guldin, James M.] US Forest Serv, USDA, So Res Stn, Hot Springs, AR 71902 USA. [Haavik, Laurel J.; Jones, Joshua S.; Galligan, Larry D.; Stephen, Fred M.] Univ Arkansas, Dept Entomol, Fayetteville, AR 72701 USA. RP Haavik, LJ (reprint author), Univ Calif Davis, Dept Entomol, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM ljhaavik@gmail.com OI Haavik, Laurel/0000-0002-7749-9095 FU University of Arkansas Division of Agriculture, Arkansas Agricultural Experiment Station; USDA Forest Service, Southern Research Station; [CA-011330124-105]; [CA-011330124-115] FX University of Arkansas Division of Agriculture, Arkansas Agricultural Experiment Station; USDA Forest Service, Southern Research Station and State and Private Forestry Grants CA-011330124-105 and CA-011330124-115 NR 59 TC 7 Z9 8 U1 5 U2 22 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X J9 FORESTRY JI Forestry PD JUL PY 2012 VL 85 IS 3 BP 341 EP 351 DI 10.1093/forestry/cps032 PG 11 WC Forestry SC Forestry GA 955PN UT WOS:000305033500005 ER PT J AU Roesch, FA Van Deusen, PC AF Roesch, Francis A. Van Deusen, Paul C. TI Monitoring forest/non-forest land use conversion rates with annual inventory data SO FORESTRY LA English DT Article ID ESTIMATORS AB The transitioning of land from forest to other uses is of increasing interest as urban areas expand and the world's population continues to grow. Also of interest, but less recognized, is the transitioning of land from other uses into forest. In this paper, we show how rates of conversion from forest to non-forest and non-forest to forest can be estimated in the US from a continuously improving publicly available annual forest inventory database, under varying definitions of conversion. Two estimation approaches are considered and contrasted. The approaches are a simple ratio estimator and the weighted maximum likelihood estimator. The latter involves a statistical procedure that incorporates the binomial nature of the indicator variables, the transition of mapped plot conditions and an intuitively appealing way to combine data from varying remeasurement periods for a temporally dependent variable. C1 [Roesch, Francis A.] US Forest Serv, USDA, So Res Stn, Asheville, NC 28804 USA. [Van Deusen, Paul C.] Natl Council Air & Stream Improvement, Tewksbury, MA 01876 USA. RP Roesch, FA (reprint author), US Forest Serv, USDA, So Res Stn, 200 WT Weaver Blvd, Asheville, NC 28804 USA. EM froesch@fs.fed.us FU USDA Forest Service, Southern Research Station, Forest Inventory and Analysis FX This work was funded by the USDA Forest Service, Southern Research Station, Forest Inventory and Analysis Program. NR 14 TC 3 Z9 3 U1 1 U2 12 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X J9 FORESTRY JI Forestry PD JUL PY 2012 VL 85 IS 3 BP 391 EP 398 DI 10.1093/forestry/cps037 PG 8 WC Forestry SC Forestry GA 955PN UT WOS:000305033500009 ER PT J AU Eldridge, DJ Koen, TB Killgore, A Huang, N Whitford, WG AF Eldridge, David J. Koen, Terry B. Killgore, Aaron Huang, Niki Whitford, Walter G. TI Animal foraging as a mechanism for sediment movement and soil nutrient development: Evidence from the semi-arid Australian woodlands and the Chihuahuan Desert SO GEOMORPHOLOGY LA English DT Article DE Foraging; Soil movement; Biopedturbation; Rodents; Vertebrates; Soil formation; Patchiness; Arid ID NORTHERN POCKET GOPHER; WESTERN MOJAVE DESERT; HARVESTER ANT NESTS; SUBTERRANEAN TERMITES; NATIVE ANIMALS; DISTURBANCE; ECOSYSTEM; RODENTS; VEGETATION; GRASSLAND AB An emerging area of interest in geomorphology over the past two decades has been the effects of biota on ecosystem processes. We examined the roles of a range of vertebrates on soil disturbance in two markedly different environments, the semi-arid woodland of eastern Australia and a Chihuahuan Desert grassland-shrubland in the south-western United States. Foraging pits of soil-disturbing vertebrates varied markedly from small scratchings of heteromyid (mainly Dipodomys spp.) rodents (1.8 x 10(-4) m(3)) to deep (1.0 x 10(-2) m(3)) excavations of the burrowing bettong (Bettongia leuseur) and greater bilby (Macrotis lagotis). Vertebrates moved substantial volumes of soil in both environments, and activity was highly temporally and spatially variable. At large spatial scales, soil disturbance by echidnas (Tachyglossus aculeatus) and Gould's sand goannas (Varanus gouldii) was substantially greater in communities dominated by shrubs, and where domestic livestock had been excluded. Heteromyid rodents tended to excavate more foraging pits in coarse-textured vegetation communities (both grasslands and shrublands). In both environments, foraging was concentrated close to perennial plants such as grass tussocks and tree canopies rather than in the interspaces. Foraging pits of Chihuahuan desert animals tended to be higher in labile carbon and support greater levels of infiltration, though this was plant community-dependent Overall our results indicate that animal foraging is an important geomorphic mechanism capable of mobilizing substantial volumes of soil in arid and semi-arid environments and with potential effects on soil function. (C) 2011 Elsevier B.V. All rights reserved. C1 [Eldridge, David J.; Huang, Niki] Univ New S Wales, Evolut & Ecol Res Ctr, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia. [Koen, Terry B.] Dept Environm Climate Change & Water, Cowra, NSW 2794, Australia. [Killgore, Aaron] New Mexico State Univ, Dept Fishery & Wildlife Sci, Las Cruces, NM 88003 USA. [Whitford, Walter G.] New Mexico State Univ, USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. RP Eldridge, DJ (reprint author), Univ New S Wales, Evolut & Ecol Res Ctr, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia. EM d.eldridge@unsw.edu.au; Terry.Koen@environment.nsw.gov.au; akillgore@onda.org FU Arid Lands Consortium FX We are grateful to the Australian Wildlife Conservancy, particularly Sarah Legge and Matt Hayward, and the Arid Lands Consortium, for continued financial and logistical support to undertake various parts of this research. We thank Kris Havstad (ARS-USDA Jornada Range Experimental Station) for allowing us access to field sites and providing office and laboratory equipment. A large number of people helped to assemble the US dataset including Verity Mathes, Ben Duval and Eric Jackson. We thank Erin Roger and David Butler for comments on an earlier draft. NR 68 TC 15 Z9 15 U1 4 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X J9 GEOMORPHOLOGY JI Geomorphology PD JUL 1 PY 2012 VL 157 SI SI BP 131 EP 141 DI 10.1016/j.geomorph.2011.04.041 PG 11 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 953UL UT WOS:000304898600012 ER PT J AU Hufkens, K Friedl, MA Keenan, TF Sonnentag, O Bailey, A O'Keefe, J Richardson, AD AF Hufkens, Koen Friedl, Mark A. Keenan, Trevor F. Sonnentag, Oliver Bailey, Amey O'Keefe, John Richardson, Andrew D. TI Ecological impacts of a widespread frost event following early spring leaf-out SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon balance; elevational gradient; frost event; northern hardwood forest; phenology ID EASTERN UNITED-STATES; CLIMATE-CHANGE; DECIDUOUS FOREST; TEMPERATE FOREST; FAGUS-CRENATA; THERMAL TIME; PHENOLOGY; MODIS; TREE; RESISTANCE AB In the spring of 2010, temperatures averaged similar to 3 similar to degrees C above the long-term mean (MarchMay) across the northeastern United States. However, in mid-to-late spring, much of this region experienced a severe frost event. The spring of 2010 therefore provides a case study on how future spring temperature extremes may affect northeastern forest ecosystems. We assessed the response of three northern hardwood tree species (sugar maple, American beech, yellow birch) to these anomalous temperature patterns using several different data sources and addressed four main questions: (1) Along an elevational gradient, how was each species affected by the late spring frost? (2) How did differences in phenological growth strategy influence their response? (3) How did the late spring frost affect ecosystem productivity within the study domain? (4) What are the potential long-term impacts of spring frost events on forest community ecology? Our results show that all species exhibited early leaf development triggered by the warm spring. However, yellow birch and American beech have more conservative growth strategies and were largely unaffected by the late spring frost. In contrast, sugar maples responded strongly to warmer temperatures and experienced widespread frost damage that resulted in leaf loss and delayed canopy development. Late spring frost events may therefore provide a competitive advantage for yellow birch and American beech at the expense of sugar maple. Results from satellite remote sensing confirm that frost damage was widespread throughout the region at higher elevations (>500 similar to m). The frost event is estimated to have reduced gross ecosystem productivity by 70153 similar to g similar to C similar to m-2, or 714% of the annual gross productivity (1061 similar to+/-similar to 82 similar to g similar to C similar to m-2) across 8753 similar to km2 of high-elevation forest. We conclude that frost events following leaf out, which are expected to become more common with climate change, may influence both forest composition and ecosystem productivity. C1 [Hufkens, Koen; Friedl, Mark A.] Boston Univ, Dept Geog & Environm, Boston, MA 02215 USA. [Keenan, Trevor F.; Sonnentag, Oliver; Richardson, Andrew D.] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Sonnentag, Oliver] Univ Montreal, Dept Geog, Montreal, PQ H3C 3J7, Canada. [Bailey, Amey] US Forest Serv, USDA, NE Res Stn, Campton, NH USA. [O'Keefe, John] Harvard Forest, Petersham, MA USA. RP Hufkens, K (reprint author), Boston Univ, Dept Geog & Environm, Boston, MA 02215 USA. EM koen.hufkens@gmail.com RI Keenan, Trevor/B-2744-2010; Richardson, Andrew/F-5691-2011; OI Keenan, Trevor/0000-0002-3347-0258; Richardson, Andrew/0000-0002-0148-6714; Hufkens, Koen/0000-0002-5070-8109 FU NASA [NNX11AE75G]; Northeastern States Research Cooperative; USDA Forest Service; Northern Research Station; National Science Foundation; Marcrosystem Biology program [EF-1065029]; US Geological Survey Status and Trends Program; US National Park Service Inventory and Monitoring Program; USA National Phenology Network, the United States Geological Survey [G10AP00129]; National Science Foundation [1114804]; National Science Foundation's LTER [DEB-0080592]; Office of Science (BER), U.S. Department of Energy (DOE) [DE-SC0004985] FX This research was supported by NASA grant number NNX11AE75G. We thank the Northeastern States Research Cooperative for supporting the PhenoCam network. Research at Bartlett Experimental Forest is partially supported by the USDA Forest Service's Northern Global Change program and Northern Research Station. Andrew Richardson acknowledges the National Science Foundation and the Marcrosystem Biology program (award EF-1065029). We thank Youngryel Ryu for assistance in the calculation of LAI. This research was partially supported by the US Geological Survey Status and Trends Program, the US National Park Service Inventory and Monitoring Program, and the USA National Phenology Network, grant number G10AP00129, from the United States Geological Survey. The contents of this article are solely the responsibility of the authors and do not necessarily represent the official views of the USGS. This manuscript is a contribution of the Hubbard Brook Ecosystem Study. Some data used in this publication were obtained by scientists of the Hubbard Brook Ecosystem Study; this publication has not been reviewed by all those scientists. Hubbard Brook is part of the Long-Term Ecological Research (LTER) network, which is supported by the National Science Foundation under Grant No. 1114804. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, PA. Research at Harvard Forest is partially supported by the National Science Foundation's LTER program (award number DEB-0080592) and the Office of Science (BER), U.S. Department of Energy (DOE) (award number DE-SC0004985). NR 65 TC 44 Z9 48 U1 10 U2 100 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2012 VL 18 IS 7 BP 2365 EP 2377 DI 10.1111/j.1365-2486.2012.02712.x PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 952VD UT WOS:000304820300024 ER PT J AU Gadino, AN Walton, VM Lee, JC AF Gadino, Angela N. Walton, Vaughn M. Lee, Jana C. TI Olfactory response of Typhlodromus pyri (Acari: Phytoseiidae) to synthetic methyl salicylate in laboratory bioassays SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE Herbivore-induced plant volatile; pest mites; plant defence; vineyards; Y-tube olfactometer ID INDUCED PLANT VOLATILES; PREDATORY MITE; TETRANYCHUS-URTICAE; BENEFICIAL INSECTS; JASMONIC ACID; SPIDER-MITES; FOOD-PLANTS; PREY; KAIROMONES; LOCATION AB The herbivore-induced plant volatile (HIPV) methyl salicylate (MeSA) is widely present in the chemical profile of several plant species and is known to attract natural enemies, including predatory mites. In this study, the response of Typhlodromus pyri, a key predator of pest mites in west coast vineyards, to synthetically produced MeSA was tested using a Y-tube olfactometer in laboratory bioassays. Six doses ranging from 0.002 to 200 mu g of MeSA diluted in 0.1 ml hexane were tested. Significantly higher proportions of T. pyri preferred MeSA at doses 0.02, 0.2 and 20 mu g. No differences in response to MeSA were detected at the highest (200 mu g), intermediate (2 mu g) and lowest (0.002 mu g) doses. Mite response to MeSA was a function of dose when fitting polynomial and logistic regression models using dose and square of the log dose prediction factors. Results indicate that synthetic MeSA may be applied to attract predatory arthropod populations in vineyards to enhance biological control of pest mites. C1 [Gadino, Angela N.; Walton, Vaughn M.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Lee, Jana C.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR USA. RP Gadino, AN (reprint author), Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. EM gadinoa@hort.oregonstate.edu FU Western Sustainable Agricultural Research and Education Program grant for graduate students; USDA Northwest Center for Small Fruits Research; Oregon Wine Board FX We would like to thank Jetske DeBoer for her time and comments in review of the manuscript. Also, we extend our gratitude to Christopher Hedstrom for assistance in rearing and transferring the mites and Elio Caceres Herrera for constructing the walking platform. The project was partially funded by the Western Sustainable Agricultural Research and Education Program grant for graduate students, the USDA Northwest Center for Small Fruits Research and the Oregon Wine Board. NR 31 TC 3 Z9 4 U1 0 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD JUL PY 2012 VL 136 IS 6 BP 476 EP 480 DI 10.1111/j.1439-0418.2011.01670.x PG 5 WC Entomology SC Entomology GA 952PI UT WOS:000304804100009 ER PT J AU Lee, ST Panter, KE Gardner, DR Green, BT Welch, KD Zhang, JJ Chang, CWT AF Lee, Stephen T. Panter, Kip E. Gardner, Dale R. Green, Benedict T. Welch, Kevin D. Zhang, Jianjun Chang, Cheng-Wei Tom TI Development of a monoclonal antibody-based ELISA for the hedgehog inhibitors cyclopamine and KAAD-cyclopamine SO JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS LA English DT Article DE Cyclopamine; Cancer; Enzyme-linked immunoassay; Hedgehog pathway ID VERATRUM CALIFORNICUM DURAND; LINKED-IMMUNOSORBENT-ASSAY; TERATOGENIC COMPOUNDS; TUMORS AB Cyclopamine (1) was isolated from the plant Veratrum californicum Durand (Liliacea) and identified as the teratogen responsible for severe craniofacial birth defects including cyclops in the offspring of sheep grazing on mountain ranges in central Idaho. More recently, cyclopamine (1) was found to inhibit the hedgehog (Hh) signaling pathway which plays a critical role in embryonic development and is implicated in several types of cancer. Thus, cyclopamine (1) and cyclopamine derivatives have been targeted as potential pharmaceutical treatments for certain cancers and other diseases associated with the Hh signaling pathway. A monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay was developed to detect and measure cyclopamine (1) and cyclopamine derivatives in biological samples. The limits of detection of the assay for cyclopamine (1), 3-keto-N-aminoethyl aminocaproyl digyrocinnamoyl-cyclopamine (8), and N-(4-L-rhamnopyranosyl-1H-1,2,3-triazol-1-yl)-methylcyclopamine (11) were 2.9 pg, 0.41 pg and 2.6 pg, respectively. This assay was also found to be useful for the detection and measurement of cyclopamine (1) in sera from mice that had been dosed with cyclopamine (1). The simple ELISA method described herein demonstrates the potential of using these techniques for the rapid screening of biological samples for the presence and levels of cyclopamine (1) and other cyclopamine derivatives that are Hh inhibitors with anticancer potential. Published by Elsevier B.V. C1 [Lee, Stephen T.; Panter, Kip E.; Gardner, Dale R.; Green, Benedict T.; Welch, Kevin D.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Zhang, Jianjun; Chang, Cheng-Wei Tom] Utah State Univ, Dept Chem & Biochem, Logan, UT 84322 USA. RP Lee, ST (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM stephen.lee@ars.usda.gov RI zhang, jianjun/H-7529-2012 NR 13 TC 2 Z9 2 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0731-7085 J9 J PHARMACEUT BIOMED JI J. Pharm. Biomed. Anal. PD JUL PY 2012 VL 66 BP 282 EP 286 DI 10.1016/j.jpba.2012.03.043 PG 5 WC Chemistry, Analytical; Pharmacology & Pharmacy SC Chemistry; Pharmacology & Pharmacy GA 953CD UT WOS:000304843600036 PM 22516681 ER PT J AU Andris, M Arias, MC Barthel, BL Bluhm, BH Bried, J Canal, D Chen, XM Cheng, P Chiappero, MB Coelho, MM Collins, AB Dash, M Davis, MC Duarte, M Dubois, MP Francoso, E Galmes, MA Gopal, K Jarne, P Kalbe, M Karczmarski, L Kim, H Martella, MB Mcbride, RS Negri, V Negro, JJ Newell, AD Piedade, AF Puchulutegui, C Raggi, L Samonte, IE Sarasola, JH See, DR Seyoum, S Silva, MC Solaro, C Tolley, KA Tringali, MD Vasemagi, A Xu, LS Zanon-Martinez, JI AF Andris, Malvina Arias, M. C. Barthel, Brandon L. Bluhm, Burton H. Bried, Joel Canal, D. Chen, X. M. Cheng, P. Chiappero, Marina B. Coelho, Manuela M. Collins, Angela B. Dash, M. Davis, Michelle C. Duarte, Margarida Dubois, Marie-Pierre Francoso, E. Galmes, M. A. Gopal, Keshni Jarne, Philippe Kalbe, Martin Karczmarski, Leszek Kim, Hun Martella, Monica B. Mcbride, Richard S. Negri, Valeria Negro, J. J. Newell, Annakay D. Piedade, Ana F. Puchulutegui, Cecilia Raggi, Lorenzo Samonte, Irene E. Sarasola, J. H. See, D. R. Seyoum, Seifu Silva, Monica C. Solaro, C. Tolley, Krystal A. Tringali, Michael D. Vasemagi, A. Xu, L. S. Zanon-Martinez, J. I. CA Mol Ecology Resources Primer Dev TI Permanent Genetic Resources added to Molecular Ecology Resources Database 1 February 2012-31 March 2012 SO MOLECULAR ECOLOGY RESOURCES LA English DT Article AB This article documents the addition of 171 microsatellite marker loci and 27 pairs of single nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bombus pauloensis, Cephalorhynchus heavisidii, Cercospora sojina, Harpyhaliaetus coronatus, Hordeum vulgare, Lachnolaimus maximus, Oceanodroma monteiroi, Puccinia striiformis f. sp. tritici, Rhea americana, Salmo salar, Salmo trutta, Schistocephalus solidus, Sousa plumbea and Tursiops aduncus. These loci were cross-tested on the following species: Aquila heliaca, Bulweria bulwerii, Buteo buteo, Buteo swainsoni, Falco rusticolus, Haliaeetus albicilla, Halobaena caerulea, Hieraaetus fasciatus, Oceanodroma castro, Puccinia graminis f. sp. Tritici, Puccinia triticina, Rhea pennata and Schistocephalus pungitii. This article also documents the addition of 27 sequencing primer pairs for Puffinus baroli and Bulweria bulwerii and cross-testing of these loci in Oceanodroma castro, Pelagodroma marina, Pelecanoides georgicus, Pelecanoides urinatrix, Thalassarche chrysostoma and Thalassarche melanophrys. C1 [Andris, Malvina] Mol Ecol Resources Editorial Off, Vancouver, BC V6T 1Z4, Canada. [Andris, Malvina; Bried, Joel] Univ Acores, Ctr IMAR, Dept Oceanog & Pescas, P-9901862 Horta, Acores, Portugal. [Arias, M. C.; Francoso, E.] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, BR-05508090 Sao Paulo, Brazil. [Barthel, Brandon L.; Collins, Angela B.; Davis, Michelle C.; Puchulutegui, Cecilia; Seyoum, Seifu; Tringali, Michael D.] Florida Fish & Wildlife Conservat Commiss, St Petersburg, FL 33701 USA. [Bluhm, Burton H.; Kim, Hun; Newell, Annakay D.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Canal, D.; Negro, J. J.] Estn Biol Donana CSIC, Dept Evolutionary Ecol, Seville 41092, Spain. [Chen, X. M.; Cheng, P.; Xu, L. S.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X. M.; See, D. R.] ARS, USDA, Wheat Genet Qual Physiol & Dis Res Unit, Pullman, WA 99164 USA. [Chiappero, Marina B.] Univ Nacl Cordoba, CONICET, RA-5000 Cordoba, Argentina. [Chiappero, Marina B.] Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, Catedra Genet Poblac & Evoluc, RA-5000 Cordoba, Argentina. [Coelho, Manuela M.; Duarte, Margarida; Piedade, Ana F.; Silva, Monica C.] Univ Lisbon, Ctr Biol Ambiental, Fac Ciencias, P-1749016 Lisbon, Portugal. [Dash, M.; Vasemagi, A.] Univ Turku, Dept Biol, Div Genet & Physiol, Turku 20014, Finland. [Dubois, Marie-Pierre; Jarne, Philippe] CNRS, UMR 5175, CEFE, F-34293 Montpellier 5, France. [Galmes, M. A.; Sarasola, J. H.; Solaro, C.; Zanon-Martinez, J. I.] Univ Nacl Pampa, CECARA, RA-6300 Santa Rosa, La Pampa, Argentina. [Galmes, M. A.; Sarasola, J. H.] Peregrine Fund, Boise, ID 83709 USA. [Gopal, Keshni] Univ Pretoria, Mammal Res Inst, ZA-8000 Cape Town, South Africa. [Gopal, Keshni; Tolley, Krystal A.] S African Natl Biodivers Inst, ZA-7735 Cape Town, South Africa. [Kalbe, Martin; Samonte, Irene E.] Max Planck Inst Evolutionary Biol, Dept Evolutionary Ecol, D-24306 Plon, Germany. [Karczmarski, Leszek] Univ Hong Kong, Swire Inst Marine Sci, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China. [Martella, Monica B.] Univ Nacl Cordoba, CONICET, RA-5000 Cordoba, Argentina. [Martella, Monica B.] Univ Nacl Cordoba, Fac Ciencias Exactas Fis & Nat, Ctr Zool Aplicada, RA-5000 Cordoba, Argentina. [Mcbride, Richard S.] NE Fisheries Sci Ctr, Natl Marine Fisheries Serv, Woods Hole, MA 02543 USA. [Negri, Valeria; Raggi, Lorenzo] Univ Perugia, Dept Appl Biol, I-06121 Perugia, Italy. [Sarasola, J. H.; Solaro, C.; Zanon-Martinez, J. I.] Consejo Nacl Invest Cient & Tecn, INCITAP, RA-6300 Santa Rosa, La Pampa, Argentina. [Vasemagi, A.] Estonian Univ Life Sci, Inst Vet Med & Anim Sci, Dept Aquaculture, EE-51014 Tartu, Estonia. RP Andris, M (reprint author), Mol Ecol Resources Editorial Off, 6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada. RI Silva, Monica/D-3300-2012; Tolley, Krystal/D-9738-2011; Canal, David/E-4401-2013; negri, valeria/G-5598-2013; Raggi, Lorenzo/G-5645-2013; Negro, Juan/I-2653-2015; Coelho, Maria/B-8672-2009; CSIC, EBD Donana/C-4157-2011; OI Arias, Maria/0000-0003-1477-101X; Silva, Monica/0000-0003-2404-3964; Canal, David/0000-0003-2875-2987; Raggi, Lorenzo/0000-0003-0484-8669; Negro, Juan/0000-0002-8697-5647; Coelho, Maria/0000-0003-3288-1693; CSIC, EBD Donana/0000-0003-4318-6602; Negri, Valeria/0000-0003-2941-121X NR 0 TC 18 Z9 18 U1 0 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD JUL PY 2012 VL 12 IS 4 BP 779 EP 781 DI 10.1111/j.1755-0998.2012.03155.x PG 3 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 956DO UT WOS:000305070300022 ER PT J AU Zhu, CW Ziska, L Zhu, JG Zeng, Q Xie, ZB Tang, HY Jia, XD Hasegawa, T AF Zhu, Chunwu Ziska, Lewis Zhu, Jianguo Zeng, Qing Xie, Zubing Tang, Haoyan Jia, Xiaodong Hasegawa, Toshihiro TI The temporal and species dynamics of photosynthetic acclimation in flag leaves of rice (Oryza sativa) and wheat (Triticum aestivum) under elevated carbon dioxide SO PHYSIOLOGIA PLANTARUM LA English DT Article ID AIR CO2 ENRICHMENT; RIBULOSE-1,5-BISPHOSPHATE REGENERATION LIMITATION; LEAF SENESCENCE; ATMOSPHERIC CO2; GROWTH; CAPACITY; PLANTS; FACE; ENHANCEMENT; RUBISCO AB In this study, we tested for the temporal occurrence of photosynthetic acclimation to elevated [CO2] in the flag leaf of two important cereal crops, rice and wheat. In order to characterize the temporal onset of acclimation and the basis for any observed decline in photosynthetic rate, we characterized net photosynthesis, gs, gm, Ci/Ca, Ci/Cc, Vcmax, Jmax, cell wall thickness, content of Rubisco, cytochrome (Cyt) f, N, chlorophyll and carbohydrate, mRNA expression for rbcL and petA, activity for Rubisco, sucrose phosphate synthase (SPS) and sucrose synthase (SS) at full flag expansion, mid-anthesis and the late grain-filling stage. No acclimation was observed for either crop at full flag leaf expansion. However, at the mid-anthesis stage, photosynthetic acclimation in rice was associated with RuBP carboxylation and regeneration limitations, while wheat only had the carboxylation limitation. By grain maturation, the decline of Rubisco content and activity had contributed to RuBP carboxylation limitation of photosynthesis in both crops at elevated [CO2]; however, the sharp decrease of Rubisco enzyme activity played a more important role in wheat. Although an increase in non-structural carbohydrates did occur during these later stages, it was not consistently associated with changes in SPS and SS or photosynthetic acclimation. Rather, over time elevated [CO2] appeared to enhance the rate of N degradation and senescence so that by late-grain fill, photosynthetic acclimation to elevated [CO2] in the flag leaf of either species was complete. These data suggest that the basis for photosynthetic acclimation with elevated [CO2] may be more closely associated with enhanced rates of senescence, and, as a consequence, may be temporally dynamic, with significant species variation. C1 [Zhu, Chunwu; Zhu, Jianguo; Zeng, Qing; Xie, Zubing; Tang, Haoyan] Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China. [Zhu, Chunwu; Hasegawa, Toshihiro] Natl Inst Agroenvironm Sci, Agrometeorol Div, Tsukuba, Ibaraki 3058604, Japan. [Ziska, Lewis] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD USA. [Jia, Xiaodong] Chinese Acad Sci, Jiangsu Inst Bot, Nanjing Bot Garden Mem Sun Yat Sen, Nanjing 210014, Peoples R China. RP Zhu, JG (reprint author), Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Peoples R China. EM jgzhu@issas.ac.cn FU International S & T Cooperation Program of China [2010DFA22770]; National Natural Science Foundation of China [40271061] FX The work was supported by the International S & T Cooperation Program of China (Grant No. 2010DFA22770), and the Key Projects of National Natural Science Foundation of China (Grant No. 40271061). The main instruments and apparatus of the FACE system were supplied by National Institute of Agro-Environmental Sciences and Agricultural Research Center of Tohoku Region (Japan). NR 36 TC 18 Z9 21 U1 6 U2 66 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD JUL PY 2012 VL 145 IS 3 BP 395 EP 405 DI 10.1111/j.1399-3054.2012.01581.x PG 11 WC Plant Sciences SC Plant Sciences GA 953LU UT WOS:000304870000003 PM 22268610 ER PT J AU Mendel, Z Protasov, A Sharon, M Zveibil, A Ben Yehuda, S O'Donnell, K Rabaglia, R Wysoki, M Freeman, S AF Mendel, Z. Protasov, A. Sharon, M. Zveibil, A. Ben Yehuda, S. O'Donnell, K. Rabaglia, R. Wysoki, M. Freeman, S. TI An Asian ambrosia beetle Euwallacea fornicatus and its novel symbiotic fungus Fusarium sp pose a serious threat to the Israeli avocado industry SO PHYTOPARASITICA LA English DT Article DE First report; Fusarium solani species complex; Persea americana ID CURCULIONIDAE; SCOLYTINAE AB The ambrosia beetle, Euwallacea fornicatus Eichhoff, was first recorded in Israel in 2009. The symbiotic fungus Fusarium sp., carried in mycacangia located in the anterior region of the female head, is responsible for the typical wilt symptoms inflicted on avocado (Persea americana Miller) trees. The beetle-fungus complex has become a serious threat to the future of the avocado industry in Israel. C1 [Mendel, Z.; Protasov, A.; Wysoki, M.] ARO, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel. [Sharon, M.; Zveibil, A.; Freeman, S.] ARO, Volcani Ctr, Dept Plant Pathol & Weed Res, IL-50250 Bet Dagan, Israel. [Ben Yehuda, S.] Minist Agr, Extens Serv, IL-50250 Bet Dagan, Israel. [O'Donnell, K.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, NCAUR, USDA, Peoria, IL 61604 USA. [Rabaglia, R.] US Forest Serv, USDA, Arlington, VA 22209 USA. RP Mendel, Z (reprint author), ARO, Volcani Ctr, Dept Entomol, IL-50250 Bet Dagan, Israel. EM zmendel@volcani.agri.gov.il FU Israeli avocado growers' organization FX The authors would like to thank Prof. Randy C. Ploetz and Prof. Jorge Pena from the Univ. of Florida, Homestead, Florida, USA, and Michael Noi, Ministry of Agriculture, Israel, for helpful discussions, and the Israeli avocado growers' organization for partial funding of this research. We also thank Stacy Sink for generating the DNA sequence data reported in this study and Nathane Orwig for running the DNA sequences in NCAUR's DNA core facility. The mention of firm names or trade products does not imply that they are endorsed or recommended by the U. S. Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. NR 8 TC 24 Z9 26 U1 1 U2 111 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0334-2123 J9 PHYTOPARASITICA JI Phytoparasitica PD JUL PY 2012 VL 40 IS 3 BP 235 EP 238 DI 10.1007/s12600-012-0223-7 PG 4 WC Plant Sciences SC Plant Sciences GA 955HH UT WOS:000305007400005 ER PT J AU Ligaba, A Maron, L Shaff, J Kochian, L Pineros, M AF Ligaba, Ayalew Maron, Lyza Shaff, Jon Kochian, Leon Pineros, Miguel TI Maize ZmALMT2 is a root anion transporter that mediates constitutive root malate efflux SO PLANT CELL AND ENVIRONMENT LA English DT Article DE Arabidopsis; aluminium; aluminium tolerance; citrate; electrophysiology; malate; mineral nutrients; organic acid anions; Xenopus laevis; ZmALMT2 ID ALUMINUM-TOLERANCE MECHANISM; ORGANIC-ACID EXUDATION; ABC TRANSPORTER; ACTIVATED CITRATE; GUARD-CELLS; ARABIDOPSIS; RESISTANCE; WHEAT; GENE; LOCALIZATION AB Root efflux of organic acid anions underlies a major mechanism of plant aluminium (Al) tolerance on acid soils. This efflux is mediated by transporters of the Al-activated malate transporter (ALMT) or the multi-drug and toxin extrusion (MATE) families. ZmALMT2 was previously suggested to be involved in Al tolerance based on joint association-linkage mapping for maize Al tolerance. In the current study, we functionally characterized ZmALMT2 by heterologously expressing it in Xenopus laevis oocytes and transgenic Arabidopsis. In oocytes, ZmALMT2 mediated an Al-independent electrogenic transport product of organic and inorganic anion efflux. Ectopic overexpression of ZmALMT2 in an Al-hypersensitive Arabidopsis KO/KD line lacking the Al tolerance genes, AtALMT1 and AtMATE, resulted in Al-independent constitutive root malate efflux which partially restored the Al tolerance phenotype. The lack of correlation between ZmALMT2 expression and Al tolerance (e.g., expression not localized to the root tip, not up-regulated by Al, and higher in sensitive versus tolerance maize lines) also led us to question ZmALMT2's role in Al tolerance. The functional properties of the ZmALMT2 transporter presented here, along with the gene expression data, suggest that ZmALMT2 is not involved in maize Al tolerance but, rather, may play a role in mineral nutrient acquisition and transport. C1 [Ligaba, Ayalew; Maron, Lyza; Shaff, Jon; Kochian, Leon; Pineros, Miguel] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. RP Pineros, M (reprint author), Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA, ARS, Ithaca, NY 14853 USA. EM miguel.pineros@ars.usda.gov RI Maron, Lyza/B-5644-2008; OI Maron, Lyza/0000-0001-6475-2357; Kochian, Leon/0000-0003-3416-089X; Pineros, Miguel/0000-0002-7166-1848 FU USDA Cooperative State Research, Education and Extension [2007-35100-18436] FX The authors would like to thank Jiping Liu, Eric Craft and Xiaomin Jia for helpful discussion and technical support. This project was supported by National Research Initiative Competitive Grant 2007-35100-18436 from the USDA Cooperative State Research, Education and Extension awarded to L.V.K and M.P. NR 65 TC 17 Z9 21 U1 4 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD JUL PY 2012 VL 35 IS 7 BP 1185 EP 1200 DI 10.1111/j.1365-3040.2011.02479.x PG 16 WC Plant Sciences SC Plant Sciences GA 956DC UT WOS:000305069000001 PM 22211473 ER PT J AU Allen, DK Laclair, RW Ohlrogge, JB Shachar-Hill, Y AF Allen, Doug K. Laclair, Russell W. Ohlrogge, John B. Shachar-Hill, Yair TI Isotope labelling of Rubisco subunits provides in vivo information on subcellular biosynthesis and exchange of amino acids between compartments SO PLANT CELL AND ENVIRONMENT LA English DT Article DE compartmentation; isotopic labelling; metabolic flux analysis; primary metabolism ID METABOLIC FLUX ANALYSIS; BRASSICA-NAPUS EMBRYOS; DEVELOPING SOYBEAN SEEDS; BETA-OXIDATION; NONAQUEOUS FRACTIONATION; ARABIDOPSIS CELLS; LIPID STORAGE; PLANTS; PROTEIN; LEAVES AB The architecture of plant metabolism includes substantial duplication of metabolite pools and enzyme catalyzed reactions in different subcellular compartments. This poses challenges for understanding the regulation of metabolism particularly in primary metabolism and amino acid biosynthesis. To explore the extent to which amino acids are made in single compartments and to gain insight into the metabolic precursors from which they derive, we used steady state 13C labelling and analysed labelling in protein amino acids from plastid and cytosol. Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a major component of green tissues and its large and small subunits are synthesized from different pools of amino acids in the plastid and cytosol, respectively. Developing Brassica napus embryos were cultured in the presence of [U-13C]-sucrose, [U-13C]-glucose, [U-13C]-glutamine or [U-13C]-alanine to generate proteins. The large subunits (LSU) and small subunits (SSU) of Rubisco were isolated and the labelling in their constituent amino acids was analysed by gas chromatography-mass spectrometry. Amino acids including alanine, glycine and serine exhibited different 13C enrichment in the LSU and SSU, demonstrating that these pools have different metabolic origins and are not isotopically equilibrated between the plastid and cytosol on the time scale of cellular growth. Potential extensions of this novel approach to other macromolecules, organelles and cell types of eukaryotes are discussed. C1 [Allen, Doug K.] ARS, USDA, Plant Genet Res Unit, St Louis, MO 63132 USA. [Allen, Doug K.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Laclair, Russell W.; Ohlrogge, John B.; Shachar-Hill, Yair] Great Lakes Bioenergy Res Ctr, E Lansing, MI 48824 USA. [Laclair, Russell W.; Ohlrogge, John B.; Shachar-Hill, Yair] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. RP Allen, DK (reprint author), ARS, USDA, Plant Genet Res Unit, St Louis, MO 63132 USA. EM doug.allen@ars.usda.gov RI Shachar-Hill, Yair/B-6165-2013; Allen, Doug/M-2836-2013 OI Shachar-Hill, Yair/0000-0001-8793-5084; Allen, Doug/0000-0001-8599-8946 FU Great Lakes Bioenergy Research Center (U.S. Department of Energy, Office of Biological and Environmental Research, Office of Science) [DE-FG02-87ER13729]; USDA [2006-35318-16661]; U.S. National Science Foundation [DBI-0701919, EF-1105249]; USDA-ARS FX This work was supported by USDA-ARS, Great Lakes Bioenergy Research Center (U.S. Department of Energy, Office of Biological and Environmental Research, Office of Science grant no. DE-FG02-87ER13729), USDA Grant 2006-35318-16661, and by U.S. National Science Foundation Plant Genome award DBI-0701919 and Emerging Frontiers award EF-1105249. NR 76 TC 23 Z9 23 U1 3 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD JUL PY 2012 VL 35 IS 7 BP 1232 EP 1244 DI 10.1111/j.1365-3040.2012.02485.x PG 13 WC Plant Sciences SC Plant Sciences GA 956DC UT WOS:000305069000005 PM 22292468 ER PT J AU Kamo, K Aebig, J Guaragna, MA James, C Hsu, HT Jordan, R AF Kamo, Kathryn Aebig, Joan Guaragna, Mary Ann James, Charity Hsu, Hei-ti Jordan, Ramon TI Gladiolus plants transformed with single-chain variable fragment antibodies to Cucumber mosaic virus SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Flower bulb crops; Virus resistance; Monoclonal antibody genes ID SPOTTED-WILT-VIRUS; NICOTIANA-TABACUM; RESISTANCE; EXPRESSION; INFECTION; CULTURES; TOBACCO; CELLS AB Plants of Gladiolus 'Peter Pears' or 'Jenny Lee' were transformed with single-chain variable fragments (scFv) to Cucumber mosaic virus (CMV) subgroup I or II. The CMV subgroup I heavy and light chain scFv fragments were placed under control of either the duplicated CaMV 35S or sugarcane Ubi9 promoters. Integration of the transgenes was verified by Southern hybridization. Plants were challenged in vitro by inoculating with purified CMV using the Helios hand-held gene gun. An initial pathogen challenge was performed using six plants/plant line for the 51 lines containing the CMV subgroup I and 37 lines with the CMV subgroup II scFv fragments. Four plant lines with the CMV subgroup I scFv and four lines with the CMV subgroup II scFv were selected for further challenging. Less than 30% of the plants challenged from two plant lines with the CMV subgroup I scFv and three plant lines with the CMV subgroup II scFv contained CMV as compared to 70% for the non-transformed control plants. Less than 40% of the plants from two other plant lines with the CMV subgroup I scFv contained CMV 1-2 months after challenge. These eight plant lines that showed resistance and two susceptible lines expressed the transgene as determined by Northern hybridization and reverse transcriptase PCR. C1 [Kamo, Kathryn; Guaragna, Mary Ann; James, Charity; Hsu, Hei-ti; Jordan, Ramon] USDA, Floral & Nursery Plants Res Unit, US Natl Arboretum, Beltsville, MD 20705 USA. [Aebig, Joan] NIAID, NIH, MVDU, Rockville, MD 20850 USA. RP Kamo, K (reprint author), USDA, Floral & Nursery Plants Res Unit, US Natl Arboretum, Beltsville, MD 20705 USA. EM Kathryn.Kamo@ars.usda.gov OI Kamo, Kathryn/0000-0001-6862-2410 NR 27 TC 3 Z9 3 U1 0 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD JUL PY 2012 VL 110 IS 1 BP 13 EP 21 DI 10.1007/s11240-012-0124-y PG 9 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA 950QR UT WOS:000304664300002 ER PT J AU Ouyang, B Fei, ZJ Joung, JG Kolenovsky, A Koh, CS Nowak, J Caplan, A Keller, WA Cui, YH Cutler, AJ Tsang, EWT AF Ouyang, Bo Fei, Zhangjun Joung, Je-Gun Kolenovsky, Allan Koh, Chushin Nowak, Jacek Caplan, Allan Keller, Wilfred A. Cui, Yuhai Cutler, Adrian J. Tsang, Edward W. T. TI Transcriptome profiling and methyl homeostasis of an Arabidopsis mutant deficient in S-adenosylhomocysteine hydrolase1 (SAHH1) SO PLANT MOLECULAR BIOLOGY LA English DT Article DE Seedling development; Microarray; S-adenosylmethionine; Methyltransferase; Methyl homeostasis; Transposons; Root hair ID ROOT-HAIR DEVELOPMENT; CELL-WALL PROTEIN; DNA METHYLATION; PLANT DEVELOPMENT; GENE; THALIANA; METABOLISM; REVEALS; GROWTH; MORPHOGENESIS AB Transcriptome profiling was conducted to detect genes whose expression is significantly changed in an Arabidopsis mutant deficient in S-adenosylhomocysteine hydrolase1 (SAHH1) during early seedling development when mutant phenotypes could be clearly observed. A total of 2,040 differentially expressed genes were identified, representing approximately 6.7 % of the 30,385 DNA oligonucleotide targets on the microarray. Among these differential expressed genes, many were mapped to pathways essential to plant growth and development including those of primary, secondary and hormone metabolisms. A significant proportion of up-regulated genes encoded transposable elements which were mapped to the centromeric and pericentromeric regions of the Arabidopsis chromosomes that were analyzed. A number of down-regulated genes were found to be involved in root hair formation, which might have contributed to the root hair defective phenotype of the mutant. Analysis of genes encoding transposable elements and those associating with root hair development indicated that these genes were highly co-expressed during seedling development. Despite SAHH1 deficiency, the expression of genes encoding methyltransferase remained largely unchanged in the sahh1 mutant. Bisulfite sequencing analysis of the transposable elements and the FWA gene revealed that their sequences in the mutant were deficient of 5-methylcytosines. Analysis of mutant genomic DNA using restriction endonucleases that were unable to cut methylated DNA suggested a genome-wide hypomethylation had occurred in the mutant. These results indicated that SAHH1 plays a critical role in methyl homeostasis, and its deficiency is a major contributing factor to the change of global gene expression, metabolic pathways and activation of transposable elements in the sahh1 mutant. C1 [Ouyang, Bo; Kolenovsky, Allan; Koh, Chushin; Nowak, Jacek; Cutler, Adrian J.; Tsang, Edward W. T.] Natl Res Council Canada NRC, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada. [Fei, Zhangjun; Joung, Je-Gun] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Fei, Zhangjun] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Caplan, Allan] Univ Idaho, Dept Microbiol Mol Biol & Biochem, Moscow, ID 83844 USA. [Keller, Wilfred A.] GenomePrairie, Saskatoon, SK S7N 3R2, Canada. [Cui, Yuhai] Agr & Agri Food Canada, London, ON N5V 4T3, Canada. RP Tsang, EWT (reprint author), Natl Res Council Canada NRC, Inst Plant Biotechnol, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9, Canada. EM Ed.Tsang@nrc-cnrc.gc.ca FU National Research Council of Canada Genomics Health Initiative 3 (GHI3); Plant Biotechnology Institute; National Research Council of Canada Genomics Health Initiative 4 (GHI4) FX We thank Mr. Chad Matsalla of Bioinformatics Dept. at PBI/NRC for his assistance involving contact information at GEO. We thank Dr. Edwin Wang, Biotechnology Research Institute-NRC, Montreal, Quebec, Canada, and Dr. Youlian Pan and Dr. Sieu Phan and their colleagues, Institute of information Technology-NRC, Ottawa, Ontario, Canada for helpful discussions, and the Plant Biotechnology Institute DNA Technology Laboratory for DNA sequencing and synthesis of oligonucleotides. We are grateful to Drs. Jitao Zou and Pierre Fobert for their critical reading of the manuscript. Funding for this project was provided by the National Research Council of Canada Genomics Health Initiatives 3 and 4 (GHI3 and GHI4), and core funding of Plant Biotechnology Institute to EWTT. This paper is National Research Council of Canada publication number 54625. NR 50 TC 5 Z9 5 U1 0 U2 11 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4412 J9 PLANT MOL BIOL JI Plant Mol.Biol. PD JUL PY 2012 VL 79 IS 4-5 BP 315 EP 331 DI 10.1007/s11103-012-9914-1 PG 17 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 953ZO UT WOS:000304913200001 PM 22555436 ER PT J AU Ukhanova, M Culpepper, T Baer, D Gordon, D Kanahori, S Valentine, J Neu, J Sun, Y Wang, X Mai, V AF Ukhanova, M. Culpepper, T. Baer, D. Gordon, D. Kanahori, S. Valentine, J. Neu, J. Sun, Y. Wang, X. Mai, V. TI Gut microbiota correlates with energy gain from dietary fibre and appears to be associated with acute and chronic intestinal diseases SO CLINICAL MICROBIOLOGY AND INFECTION LA English DT Article DE Colorectal cancer; dietary fibre; microbiota; necrotizing enterocolitis ID OBESITY AB Clin Microbiol Infect 2012; 18 (Suppl. 4): 6266 Abstract Improvements in high-throughput sequencing technologies have spurred a large number of studies aimed at obtaining a better understanding of the composition and the dynamics in gut microbiota and its associations with various human diseases, especially those in the intestinal tract. Here we briefly summarize results from three different such studies from our group, all of which used 454 based high-throughput 16S rRNA sequence analysis combined with other microbiota profiling methods to determine faecal microbiota composition. In the first study, a controlled feeding trial, we establish that energy gain from the consumption of up to 50 g/day of a resistant maltodextrin depends on the prevalent microbiota composition. Over time, resistant maltodextrin supplementation increased the proportion of total faecal bacteria as well as potentially beneficial bifidobacteria. Thus, energy gain from resistant maltodextrin in an individual appears to vary over time and depend on the adaptation of gut microbiota. We then illustrate the power of molecular tools for identifying (i) distortions in early microbiota development in pre-term infants and the presence of potentially novel pathogens contributing to necrotizing enterocolitis and (ii) a specific microbiota signature, based on discriminant analysis of the 16S rRNA sequences, that correlates with the prevalence of an early risk marker associated with colorectal carcinogenesis, intestinal adenoma, in elderly adults. C1 [Mai, V.] Univ Florida, Dept Microbiol & Cell Sci, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Baer, D.] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. [Gordon, D.; Kanahori, S.] Matsutani Chem Ind Co Ltd, Itami, Hyogo, Japan. [Valentine, J.] Div Gastroenterol, Gainesville, FL USA. [Neu, J.] Div Pediat, Gainesville, FL USA. [Sun, Y.] ICBR Univ Florida, Gainesville, FL USA. RP Mai, V (reprint author), Univ Florida, Dept Microbiol & Cell Sci, Emerging Pathogens Inst, 2055 Mowry Rd, Gainesville, FL 32611 USA. EM vmai@ufl.edu FU US Department of Agriculture; Matsutani Chemical Industry Co. Ltd.; NIH [RO1 HD 059143]; American Cancer Society [MRSGT CCE-107301]; NSF [DBI-1062362] FX The RM studies were funded by the US Department of Agriculture and the Matsutani Chemical Industry Co. Ltd. The ongoing pre-term infant study is funded by a grant from the NIH RO1 HD 059143 to Dr J. Neu. and Dr V. Mai was funded by a mentored research scholar award from the American Cancer Society (MRSGT CCE-107301) for the colonoscopy study. Work on bioinformatics for 16S rRNA sequence analysis is funded by NSF grant DBI-1062362 to Drs Sun and Mai. NR 16 TC 4 Z9 5 U1 2 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1198-743X J9 CLIN MICROBIOL INFEC JI Clin. Microbiol. Infect. PD JUL PY 2012 VL 18 SU 4 SI SI BP 62 EP 66 DI 10.1111/j.1469-0691.2012.03859.x PG 5 WC Infectious Diseases; Microbiology SC Infectious Diseases; Microbiology GA 951ZN UT WOS:000304759600017 PM 22647053 ER PT J AU Burkey, KO Booker, FL Ainsworth, EA Nelson, RL AF Burkey, Kent O. Booker, Fitzgerald L. Ainsworth, Elizabeth A. Nelson, Randall L. TI Field assessment of a snap bean ozone bioindicator system under elevated ozone and carbon dioxide in a free air system SO ENVIRONMENTAL POLLUTION LA English DT Article DE Bioindicator; Carbon dioxide; Free air concentration enrichment (FACE); Ozone; Snap bean ID PHASEOLUS-VULGARIS L.; CO2 ENRICHMENT FACE; TROPOSPHERIC OZONE; WHITE CLOVER; YIELD RESPONSES; UNITED-STATES; SEED YIELD; PHOTOSYNTHESIS; SENSITIVITY; IMPACT AB Ozone-sensitive (S156) and -tolerant (R123 and R331) genotypes of snap bean (Phaseolus vulgaris L.) were tested as a plant bioindicator system for detecting O-3 effects at current and projected future levels of tropospheric O-3 and atmospheric CO2 under field conditions. Plants were treated with ambient air, 1.4x ambient O-3 and 550 ppm CO2 separately and in combination using Free Air Concentration Enrichment technology. Under ambient O-3 concentrations pod yields were not significantly different among genotypes. Elevated O-3 reduced pod yield for S156 (63%) but did not significantly affect yields for R123 and R331. Elevated CO2 at 550 ppm alone did not have a significant impact on yield for any genotype. Amelioration of the O-3 effect occurred in the O-3 + CO2 treatment. Ratios of sensitive to tolerant genotype pod yields were identified as a useful measurement for assessing O-3 impacts with potential applications in diverse settings including agricultural fields. Published by Elsevier Ltd. C1 [Burkey, Kent O.; Booker, Fitzgerald L.] N Carolina State Univ, Plant Sci Res Unit, USDA ARS, Raleigh, NC 27607 USA. [Burkey, Kent O.; Booker, Fitzgerald L.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27607 USA. [Ainsworth, Elizabeth A.] Univ Illinois, Global Change & Photosynth Res Unit, USDA ARS, Urbana, IL 61801 USA. [Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Nelson, Randall L.] Univ Illinois, Soybean Maize Germplasm Pathol & Genet Res Unit, USDA ARS, Urbana, IL 61801 USA. [Nelson, Randall L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Burkey, KO (reprint author), N Carolina State Univ, Plant Sci Res Unit, USDA ARS, 3127 Ligon St, Raleigh, NC 27607 USA. EM Kent.Burkey@ars.usda.gov; Fitz.Booker@ars.usda.gov; Lisa.Ainsworth@ars.usda.gov; Randall.Nelson@ars.usda.gov NR 33 TC 7 Z9 8 U1 1 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUL PY 2012 VL 166 BP 167 EP 171 DI 10.1016/j.envpol.2012.03.020 PG 5 WC Environmental Sciences SC Environmental Sciences & Ecology GA 948ON UT WOS:000304512600021 PM 22507388 ER PT J AU Pretorius, ZA Jin, Y Bender, CM Herselman, L Prins, R AF Pretorius, Z. A. Jin, Y. Bender, C. M. Herselman, L. Prins, R. TI Seedling resistance to stem rust race Ug99 and marker analysis for Sr2, Sr24 and Sr31 in South African wheat cultivars and lines SO EUPHYTICA LA English DT Article DE Puccinia graminis f. sp tritici; Resistance; Sr genes; Triticum aestivum; Ug99 ID F-SP TRITICI; GENE SR2; VIRULENCE; TTKS; AUSTRALIA AB The appearance and spread of races of Puccinia graminis f. sp. tritici with virulence for the Sr31 resistance gene has renewed interest in breeding for durable resistance to stem rust of wheat. Since the occurrence of stem rust has been low in South Africa until the detection of race TTKSF in 2000, breeding for resistance to this disease has not been a primary objective. The aim of this study was to test bread wheat cultivars and lines at the seedling stage for their infection response to stem rust, thus determining their level of resistance or vulnerability. A collection of 65 bread wheat entries was tested with one USA race, two Eastern African races, and three South African races of P. graminis f. sp. tritici. The Eastern African and South African races all belong to the Ug99 lineage. The cultivars Duzi, Caledon, Elands, PAN 3364, PAN 3191, SST 047, SST 399, and Steenbras produced resistant infection types (IT < 3) to all races. The molecular marker Sr24#50 indicated the presence of Sr24 in 12 entries. In cultivars resistant to TTTTF, TTKSF, and TTKSP but susceptible to TTKSK and PTKST, the iag95 DNA marker indicated the presence of Sr31 in five wheat lines. Using the cleaved amplified polymorphic sequence marker csSr2, Sr2 was detected in PAN 3377, Inia, and Steenbras. Few South African wheat cultivars appear to have a broad-based resistance to stem rust, as 88% of the entries were susceptible as seedlings to at least one of the races tested. Diversification of resistance sources and more directed breeding for stem rust resistance are needed in South Africa. C1 [Pretorius, Z. A.; Bender, C. M.; Herselman, L.; Prins, R.] Univ Orange Free State, Dept Plant Sci, ZA-9300 Bloemfontein, South Africa. [Jin, Y.] Univ Minnesota, ARS, USDA, Cereal Dis Lab, St Paul, MN 55108 USA. [Prins, R.] CenGen, ZA-6850 Worcester, South Africa. RP Pretorius, ZA (reprint author), Univ Orange Free State, Dept Plant Sci, POB 339, ZA-9300 Bloemfontein, South Africa. EM PretorZA@ufs.ac.za FU Winter Cereal Trust; National Research Foundation FX The authors thank Debbie Snyman and Elsabet Wessels for technical assistance, Afgri, ARC-Small Grain Institute, Sensako and Pannar for seed, and the Winter Cereal Trust and National Research Foundation for funding. NR 30 TC 10 Z9 11 U1 0 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 1 BP 15 EP 23 DI 10.1007/s10681-011-0476-0 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 947VD UT WOS:000304459600002 ER PT J AU Basnet, BR Glover, KD Ibrahim, AMH Yen, Y Chao, SM AF Basnet, Bhoja R. Glover, Karl D. Ibrahim, Amir M. H. Yen, Yang Chao, Shiaoman TI A QTL on chromosome 2DS of 'Sumai 3' increases susceptibility to Fusarium head blight in wheat SO EUPHYTICA LA English DT Article DE Disease index; Fusarium damaged kernels; Recombinant inbred lines; DArT; SSR ID ARRAYS TECHNOLOGY DART; BREAD WHEAT; RESISTANCE QTL; SPRING WHEAT; D-GENOME; POPULATIONS; BARLEY; MAP; LOCI; SCAB AB Much effort has been invested in identifying molecular markers in wheat (Triticum aestivum L.) linked to quantitative trait loci (QTL) that confer resistance to Fusarium head blight (FHB) caused by Fusarium graminearum Schwabe [teleomorph Gibberella zeae (Schwein) Petch]. Even after several generations of crossing and selection by many wheat breeding programs, resistance of the Chinese spring wheat cultivar 'Sumai 3' (PI 481542) remains among the most effective. It therefore seems that undocumented resistance QTL present in Sumai 3 were not detected in various mapping studies. Using an extremely susceptible Tibetan landrace ('Y1193-6'; unknown pedigree) in the creation of a mapping population with Sumai 3, the objective of this research was to identify undocumented resistance QTL in Sumai 3. This was accomplished through collecting disease index (DI) and Fusarium damaged kernel (FDK) phenotypic values along with 305 Diversity Array Technology (DArT) and 52 Simple Sequence Repeat (SSR) marker genotypes on 160 F-2:6 recombinant inbred lines (RILs). Disease response evaluations were based on four (two greenhouse and two field) experiments where spray inoculation methods were used. Three QTL were identified on chromosome arms 3BS, 6BL and 2DS explaining 26.1, 10.7 and 18.9% of the phenotypic variation for DI, respectively. The same QTL were also significantly associated with reduced FDK scores and explained 28.0, 11.0 and 23.0% of phenotypic variation. Lines within the mapping population were placed in eight categories with respect to their various QTL combinations. Lines with no QTL were the most susceptible, whereas those with the Sumai 3-derived 3BS and 6BL QTL combined with the 2DS QTL from Y1193-6 were the most resistant. Though the 3BS and 6BL QTL are well-documented, the 2DS resistance QTL, which was contributed by the susceptible parent, confers increased susceptibility when derived from Sumai 3. In this study no new FHB QTL from Sumai 3 was discovered, but results suggest that Sumai 3 contains a QTL for susceptibility on chromosome arm 2DS. Selection against this QTL may potentially increase resistance levels among Sumai 3-derived populations. C1 [Basnet, Bhoja R.; Ibrahim, Amir M. H.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Glover, Karl D.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Yen, Yang] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Chao, Shiaoman] ARS, USDA, Biosci Res Lab, Fargo, ND 58102 USA. RP Basnet, BR (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM bbasnet@ag.tamu.edu NR 47 TC 7 Z9 8 U1 1 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 1 BP 91 EP 101 DI 10.1007/s10681-011-0495-x PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 947VD UT WOS:000304459600008 ER PT J AU Agostinelli, AM Clark, AJ Brown-Guedira, G Van Sanford, DA AF Agostinelli, Andres M. Clark, Anthony J. Brown-Guedira, Gina Van Sanford, David A. TI Optimizing phenotypic and genotypic selection for Fusarium head blight resistance in wheat SO EUPHYTICA LA English DT Article DE Deoxynivalenol; Fusarium head blight; Marker assisted selection; Quantitative trait locus; Wheat breeding ID RED WINTER-WHEAT; TRAIT LOCI QTL; SPRING WHEAT; DEOXYNIVALENOL ACCUMULATION; MOLECULAR MARKERS; DON CONTENT; MAJOR QTL; SCAB; BARLEY; HERITABILITY AB Fusarium head blight (FHB), or head scab, is an economically important disease of wheat (Triticum aestivum L.). In developing FHB-resistant soft winter wheat cultivars, breeders have relied on phenotypic selection, marker assisted selection (MAS), or a combination of the two. The objectives of this study were to estimate heritability of resistance in a resistant x susceptible cross and to simulate selection in order to determine the optimal combination of phenotypic and genotypic selection. F-2 derived lines from the cross of KY93C-1238-17-2 (high yielding, susceptible) x VA01W-476 (resistant line with two exotic quantitative trait loci (QTL) and additional resistance) were grown under artificial inoculation in scab nurseries at Lexington (2007 and 2008) and Princeton (2008), KY. Visual symptoms were estimated on a 1-3 scale; percentage Fusarium damaged kernels (FDK), and deoxynivalenol (DON) concentration were measured. VA01W-476 contributed resistance alleles at two major QTL: Fhb1 and a QTL on chromosome 2DL, QFhs.nau-2DL. In this genetic background, the effect of QFhs.nau-2DL was more pronounced than that of Fhb1: 55 vs. 25% DON reduction and 40 vs. 32% FDK reduction. Genotypic selection based on both QTL was equivalent to phenotypic selection of the most resistant 28% of the population for DON and the most resistant 24% of the population for FDK. We propose that an initial round of phenotypic selection at moderate selection intensity will enrich the population with major QTL resistance alleles while maintaining variation at minor scab resistance loci and for other traits in general. Genotyping can then be used to extract lines whose phenotypic worth has been demonstrated and which are homozygous for resistance alleles at the major QTL. C1 [Clark, Anthony J.; Van Sanford, David A.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Agostinelli, Andres M.] Limagrain S Amer, Chacabuco, Argentina. [Brown-Guedira, Gina] ARS, USDA, Raleigh, NC USA. RP Van Sanford, DA (reprint author), Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. EM dvs@uky.edu FU U.S. Department of Agriculture [59-0790-4-127, 59-0206-9-054] FX The investigation reported in this paper (10-06-052) is in connection with a project of the Kentucky Agric. Exp. Stn. and is published with the approval of the director. This material is based upon work supported by the U.S. Department of Agriculture, under Agreement Nos. 59-0790-4-127 and 59-0206-9-054. This is a cooperative project with the U.S. Wheat & Barley Scab Initiative. NR 46 TC 13 Z9 14 U1 5 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2012 VL 186 IS 1 BP 115 EP 126 DI 10.1007/s10681-011-0499-6 PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 947VD UT WOS:000304459600010 ER PT J AU Prom, LK Perumal, R Erattaimuthu, SR Little, CR No, EG Erpelding, JE Rooney, WL Odvody, GN Magill, CW AF Prom, L. K. Perumal, R. Erattaimuthu, S. R. Little, C. R. No, E. G. Erpelding, J. E. Rooney, W. L. Odvody, G. N. Magill, C. W. TI Genetic diversity and pathotype determination of Colletotrichum sublineolum isolates causing anthracnose in sorghum SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE AFLP; Fungal plant pathogen; Host differentials ID ARBITRARILY PRIMED PCR; GRAMINICOLA; RESISTANCE; MARKERS; DNA; VIRULENCE; MAP AB Amplified fragment length polymorphism (AFLP) based genetic diversity was analyzed for 232 Colletotrichum sublineolum isolates collected between 2002 and 2004 from three geographically distinct regions of Texas, and from Arkansas, Georgia, and Puerto Rico. Results revealed significant levels of polymorphism (59%) among the isolates. Even so, genetic similarity between isolates was high, ranging from 0.78 to 1.00. Clustering of similar isolates did not correlate with either geographic origin or year of collection. Pathotypes of 20 of the isolates were determined using 14 sorghum lines previously used in Brazil and the United States and 4 from Sudan. Seventeen new pathotypes were established from the 18 isolates that gave uniform and consistent reactions on all host differentials over 2 years of greenhouse testing. Differentials BTx378 and QL3 were resistant to all isolates while BTx623 and TAM428 were universally susceptible both years. Each of these lines had shown differential responses in prior studies indicating that the pathogen population has sufficient diversity to adapt rapidly to changes in resistant host lines deployed. When the 2-step pathotype classification scheme was used, the 18 isolates examined in this study were placed in four pathotype groups (A, C, D and G), which would further then be separated into ten distinct pathotypes. Common sets of differentials and a standardized nomenclature will allow for comparison to be made among pathotypes of C. sublineolum detected from different regions and also could help direct planting of appropriate sorghum lines and aid in the development of more durable forms of resistance. C1 [Magill, C. W.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Prom, L. K.] USDA ARS, So Plains Agr Res Ctr, College Stn, TX USA. [Perumal, R.] Kansas State Univ, Agr Res Ctr, Hays, KS USA. [Erattaimuthu, S. R.; Little, C. R.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [No, E. G.] Texas A&M Univ, Inst Plant Genom & Biotechnol, College Stn, TX 77843 USA. [Erpelding, J. E.] USDA ARS, Crop Genet Res Unit, Stoneville, MS USA. [Rooney, W. L.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. [Odvody, G. N.] Texas AgriLife Sci, Dept Plant Pathol & Microbiol, Corpus Christi, TX USA. RP Magill, CW (reprint author), Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. EM c-magill@tamu.edu FU Texas Agrilife Research; USDA FX Support from Texas Agrilife Research and the USDA is gratefully acknowledged. This paper is Contribution No. 11-247-J from the Kansas Agricultural Experiment Station, Manhattan. NR 43 TC 7 Z9 8 U1 1 U2 26 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2012 VL 133 IS 3 BP 671 EP 685 DI 10.1007/s10658-012-9946-z PG 15 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 947QW UT WOS:000304445600018 ER PT J AU Lakshman, DK Pandey, R Kamo, K Bauchan, G Mitra, A AF Lakshman, Dilip K. Pandey, Ruchi Kamo, Kathryn Bauchan, Gary Mitra, Amitava TI Genetic transformation of Fusarium oxysporum f.sp gladioli with Agrobacterium to study pathogenesis in Gladiolus SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Enhanced green fluorescent protein; Enhanced yellow fluorescent protein; Enhanced cyano fluorescent protein; Gladiolus corm rot; Histopathological investigations ID F-SP GLADIOLI; TUMEFACIENS-MEDIATED TRANSFORMATION; INSERTIONAL MUTAGENESIS; RESISTANCE; FUNGUS; ROT; PATHOGENICITY; LYCOPERSICI; DISEASE; PROTEIN AB Fusarium rot caused by Fusarium oxysporum f.sp. gladioli (Fog) is one of the most serious diseases of Gladiolus, both in the field and in bulbs in storage. In order to study the mechanisms of pathogenesis of this fungus, we have transformed Fog with Agrobacterium tumefaciens binary vectors containing the hygromycin B phosphotransferase (hph) gene and fluorescence reporter genes EGFP (green), EYFP (yellow) or ECFP (cyan) using the AGL-1 strain of A. tumefaciens. Hygromycin B (100 mu g/ml) resistant colonies were observed only when acetosyringone was added to the co-cultivation medium. Transformed colonies are more clearly visible when co-cultivated on cellophane membrane than on Hybond -N+ membrane. Transformed lines were stably maintained through four serial passages on medium containing hygromycin B, and they expressed green, yellow or cyano fluorescence. PCR with hph-specific primers and Southern blotting with an hph-specific probe were positive for Hyg(R) lines but not for the untransformed isolate. The cyano fluorescence of the ECFP-transformed isolate was clearly distinguishable from the green autofluorescence of Gladiolus roots, signifying the potential of these lines for further histopathological investigations. Transformed lines will be useful for identifying pathogenicity related genes, screening transgenic resistance, and in studies of host-pathogen interactions. C1 [Lakshman, Dilip K.; Pandey, Ruchi; Kamo, Kathryn] USDA ARS, Floral & Nursery Plants Res Unit, BARC, Beltsville, MD 20705 USA. [Lakshman, Dilip K.; Pandey, Ruchi] USDA ARS, Sustainable Agr Syst Lab, BARC, Beltsville, MD 20705 USA. [Bauchan, Gary] USDA ARS, Electron & Confocal Microscopy Unit, BARC, Beltsville, MD 20705 USA. [Mitra, Amitava] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Lakshman, DK (reprint author), USDA ARS, Floral & Nursery Plants Res Unit, BARC, Beltsville, MD 20705 USA. EM Dilip.Lakshman@ars.usda.gov OI Kamo, Kathryn/0000-0001-6862-2410 NR 38 TC 7 Z9 9 U1 0 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2012 VL 133 IS 3 BP 729 EP 738 DI 10.1007/s10658-012-9953-0 PG 10 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 947QW UT WOS:000304445600022 ER PT J AU Lin, LM Li, RH Mock, R Kinard, G AF Lin, Liming Li, Ruhui Mock, Ray Kinard, Gary TI One-step multiplex RT-PCR for simultaneous detection of four pome tree viroids SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Apscaviroid; Apple dimple fruit viroid; Apple fruit crinkle viroid; Apple scar skin viroid; Multiplex RT-PCR; Pear blister canker viroid ID POLYMERASE-CHAIN-REACTION; FRUIT-TREES; HOST-RANGE; APPLE; IDENTIFICATION; INFECTIONS; VIRUSES; RNA AB Apple scar skin viroid (ASSVd), Apple dimple fruit viroid (ADFVd), Apple fruit crinkle viroid (AFCVd), and Pear blister canker viroid (PBCVd) infect pome fruit trees. These viroids are important quarantine pathogens for the international movement of pome germplasm. A single-step multiplex reverse transcription polymerase chain reaction assay (mRT-PCR) was developed for the simultaneous detection of these viroids. Four pairs of primers specific for each of the four viroids were used to amplify PCR products of different sizes that can be resolved by agarose gel electrophoresis. Amplification of a plant 18S rRNA was included in the assay as an internal control. Amplicons of 371 bp (AFCVd), 270 bp (ADFVd), 186 bp (ASSVd), 120 bp (PBCVd), and 844 bp (18S rRNA) were obtained in both uniplex and mRT-PCR assays. The identities of the amplification products were confirmed by sequencing. The specificities and limits of detection for all four viroids by uniplex and mRT-PCR assays were comparable. The assay was further validated using samples from pome trees inoculated with all four viroids, as well as field samples from commercial orchards in Colorado. All four viroids were detected from inoculated pear trees and up to three viroids were detected from inoculated apple trees. This is a simple, rapid and cost-effective technique to detect these four viroids in fruit trees. The procedure is especially applicable to certification and quarantine programs, where numerous samples must be tested for all four viroids. C1 [Lin, Liming; Li, Ruhui; Mock, Ray; Kinard, Gary] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. RP Kinard, G (reprint author), USDA ARS, Natl Germplasm Resources Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM gary.kinard@ars.usda.gov NR 27 TC 5 Z9 5 U1 2 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2012 VL 133 IS 3 BP 765 EP 772 DI 10.1007/s10658-012-9956-x PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 947QW UT WOS:000304445600025 ER PT J AU Mensing, S Korfmacher, J Minckley, T Musselman, R AF Mensing, Scott Korfmacher, John Minckley, Thomas Musselman, Robert TI A 15,000 year record of vegetation and climate change from a treeline lake in the Rocky Mountains, Wyoming, USA SO HOLOCENE LA English DT Article DE climate change; conifer stomata; Holocene; pollen; Rocky Mountains; treeline; United States; Wyoming ID ENVIRONMENTAL-CHANGE; RADIOCARBON AGE; NORTH-AMERICA; MODERN POLLEN; HOLOCENE; COLORADO; RANGE; ESTABLISHMENT; FLUCTUATIONS; PALEOCLIMATE AB Future climate projections predict warming at high elevations that will impact treeline species, but complex topographic relief in mountains complicates ecologic response, and we have a limited number of long-term studies examining vegetation change related to climate. In this study, pollen and conifer stomata were analyzed from a 2.3 m sediment core extending to 15,330 cal. yr BP recovered from a treeline lake in the Rocky Mountains of Wyoming. Both pollen and stomata record a sequence of vegetation and climate change similar in most respects to other regional studies, with sagebrush steppe and lowered treeline during the Late Pleistocene, rapid upward movement of treeline beginning about 11,500 cal. yr BP, treeline above modern between similar to 9000 and 6000 cal. yr BP, and then moving downslope similar to 5000 cal. yr BP, reaching modern limits by similar to 3000 cal. yr BP. Between 6000 and 5000 cal. yr BP sediments become increasingly organic and sedimentation rates increase. We interpret this as evidence for lower lake levels during an extended dry period with warmer summer temperatures and treeline advance. The complex topography of the Rocky Mountains makes it challenging to identify regional patterns associated with short term climatic variability, but our results contribute to gaining a better understanding of past ecologic responses at high elevation sites. C1 [Mensing, Scott] Univ Nevada, Reno, NV 89557 USA. [Korfmacher, John; Musselman, Robert] US Forest Serv, Morgantown, WV USA. [Minckley, Thomas] Univ Wyoming, Laramie, WY 82071 USA. RP Mensing, S (reprint author), Univ Nevada, 201 Mackay Sci Hall, Reno, NV 89557 USA. EM smensing@unr.edu OI Minckley, Thomas/0000-0002-6483-013X FU United States Forest Service, Rocky Mountain Research Station, Fort Collins, Colorado; National Science Foundation [EPS-0447681] FX This work was supported by the United States Forest Service, Rocky Mountain Research Station, Fort Collins, Colorado and sabbatical development leave from the University of Nevada, Reno to SAM. Surface coring by TM was supported by the National Science Foundation (EPS-0447681). NR 46 TC 8 Z9 8 U1 0 U2 36 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0959-6836 J9 HOLOCENE JI Holocene PD JUL PY 2012 VL 22 IS 7 BP 739 EP 748 DI 10.1177/0959683611430339 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 950SF UT WOS:000304668500002 ER PT J AU Milner, MJ Craft, E Yamaji, N Koyama, E Ma, JF Kochian, LV AF Milner, Matthew J. Craft, Eric Yamaji, Naoki Koyama, Emi Ma, Jian Feng Kochian, Leon V. TI Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation SO NEW PHYTOLOGIST LA English DT Article DE cadmium (Cd) hyperaccumulation; Noccaea caerulescens; zinc (Zn) hyperaccumulation; ZNT1; Zn uptake ID THLASPI-CAERULESCENS; MOLECULAR PHYSIOLOGY; METAL TRANSPORTER; CADMIUM UPTAKE; ZINC; EXPRESSION; ACCUMULATION; TOLERANCE; YEAST; ROOT AB In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem. C1 [Milner, Matthew J.; Craft, Eric; Kochian, Leon V.] Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Milner, Matthew J.] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Yamaji, Naoki; Koyama, Emi; Ma, Jian Feng] Okayama Univ, Inst Plant Sci & Resources, Kurashiki, Okayama 7100046, Japan. RP Kochian, LV (reprint author), Cornell Univ, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. EM lvk1@cornell.edu RI YAMAJI, Naoki/B-2524-2011; Ma, Jian Feng/C-7213-2009; Milner, Matthew/M-2430-2014 OI Kochian, Leon/0000-0003-3416-089X; YAMAJI, Naoki/0000-0002-7499-3004; Milner, Matthew/0000-0001-9184-7423 NR 26 TC 18 Z9 18 U1 1 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X J9 NEW PHYTOL JI New Phytol. PD JUL PY 2012 VL 195 IS 1 BP 113 EP 123 DI 10.1111/j.1469-8137.2012.04144.x PG 11 WC Plant Sciences SC Plant Sciences GA 947RW UT WOS:000304448500015 PM 22524643 ER PT J AU VanLoocke, A Betzelberger, AM Ainsworth, EA Bernacchi, CJ AF VanLoocke, Andy Betzelberger, Amy M. Ainsworth, Elizabeth A. Bernacchi, Carl J. TI Rising ozone concentrations decrease soybean evapotranspiration and water use efficiency whilst increasing canopy temperature SO NEW PHYTOLOGIST LA English DT Article DE evapotranspiration; free air concentration enrichment (FACE); surface energy balance; tropospheric ozone; vegetation-climate interactions; water use efficiency (WUE) ID AIR CO2 ENRICHMENT; GROUND-LEVEL OZONE; ENERGY-BALANCE; CROP YIELDS; BIOENERGY CROPS; UNITED-STATES; GROWTH; CARBON; PHOTOSYNTHESIS; ELEVATION AB Here, we investigated the effects of increasing concentrations of ozone ([O3]) on soybean canopy-scale fluxes of heat and water vapor, as well as water use efficiency (WUE), at the Soybean Free Air Concentration Enrichment (SoyFACE) facility. Micrometeorological measurements were made to determine the net radiation (Rn), sensible heat flux (H), soil heat flux (G0) and latent heat flux (?ET) of a commercial soybean (Glycine max) cultivar (Pioneer 93B15), exposed to a gradient of eight daytime average ozone concentrations ranging from approximately current (c. 40 ppb) to three times current (c. 120 ppb) levels. As [O3] increased, soybean canopy fluxes of ?ET decreased and H increased, whereas Rn and G0 were not altered significantly. Exposure to increased [O3] also resulted in warmer canopies, especially during the day. The lower ?ET decreased season total evapotranspiration (ET) by c. 26%. The [O3]-induced relative decline in ET was half that of the relative decline in seed yield, driving a 50% reduction in seasonal WUE. These results suggest that rising [O3] will alter the canopy energy fluxes that drive regional climate and hydrology, and have a negative impact on productivity and WUE, key ecosystem services. C1 [Betzelberger, Amy M.; Ainsworth, Elizabeth A.; Bernacchi, Carl J.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [VanLoocke, Andy] Univ Illinois, Dept Atmospher Sci, Urbana, IL 61801 USA. [Ainsworth, Elizabeth A.; Bernacchi, Carl J.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. RP Bernacchi, CJ (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM bernacch@illinois.edu OI Bernacchi, Carl/0000-0002-2397-425X FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2010-65114-20355]; USDA-ARS FX The authors would like to acknowledge Don Ort in managing the SoyFACE facility, Tim Mies and Charlie Mitsdarfer in maintaining the ozone treatment, Benjamin Castellani and Pat Schmitz in assisting with technical aspects of the micrometerological measurements and Randy Nelson in assisting with planting and harvesting. We would also like to thank the three anonymous referees for their helpful comments. This work was supported in part by the Agriculture and Food Research Initiative competitive grant no. 2010-65114-20355 from the USDA National Institute of Food and Agriculture to E. A. A., as well as the USDA-ARS. NR 44 TC 10 Z9 10 U1 4 U2 87 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JUL PY 2012 VL 195 IS 1 BP 164 EP 171 DI 10.1111/j.1469-8137.2012.04152.x PG 8 WC Plant Sciences SC Plant Sciences GA 947RW UT WOS:000304448500019 PM 22524697 ER PT J AU Bacon, CW Hinton, DM Mitchell, TR Snook, ME Olubajo, B AF Bacon, Charles W. Hinton, Dorothy M. Mitchell, Trevor R. Snook, Maurice E. Olubajo, Babatunde TI Characterization of endophytic strains of Bacillus mojavensis and their production of surfactin isomers SO BIOLOGICAL CONTROL LA English DT Article DE Bacillus mojavensis; Bacterial endophyte; Biocontrol; Fusarium verticillioides; Endophytic bacterium; Fumonisin; Maize endophyte; Surfactin ID SEQUENCE-BASED PCR; BIOLOGICAL-CONTROL; SP-NOV; SUBTILIS; LIPOPEPTIDES AB Bacillus subtilis consists of a large collection of strains from which several cryptic species have been delineated, and most of these along with strains within the species are important biocontrol agents. Bacillus mojavensis, a species recently distinguished from this broad B. subtilis group of bacteria, was determined to be endophytic and to have biocontrol potential due to its inhibition of the maize mycotoxic fungus Fusarium verticillioides. Recently, we reported that a patented strain B. mojavensis produced the biosurfactant Leu(7)-surfactin that was inhibitory to fungi. In this study, the first objective was to validate the identity of strains, and analyze genotypically a collection of B. mojavensis strains, which involved analysis of repetitive-PCR amplified Bacillus DNA sequences with a PCR genotyping bar system. In an effort to understand further surfactin production, a second objective was to screen this collection of B. mojavensis strains for surfactin analogs. The results indicated that all strains are valid B. mojavensis, and that there was genotypic diversity among strains from the great deserts. Further, the study established that most strains can produce a mixture of surfactins that was comprised of acyl chain lengths ranging from C-11 to C-17. These experiments indentified high producers of C-15 surfactin, the most biologically active isoform. However, the in vitro inhibition observed did not necessarily relate to total surfactin concentrations, suggesting a complex mechanism for inhibition and/or the presence of other unknown factors. Published by Elsevier Inc. C1 [Bacon, Charles W.; Hinton, Dorothy M.; Mitchell, Trevor R.; Snook, Maurice E.; Olubajo, Babatunde] ARS, USDA, Toxicol & Mycotoxin Res Unit, Russell Res Ctr, Athens, GA 30604 USA. RP Bacon, CW (reprint author), ARS, USDA, Toxicol & Mycotoxin Res Unit, Russell Res Ctr, 950 Coll Stn Rd,POB 5677, Athens, GA 30604 USA. EM Charles.bacon@ars.usda.gov NR 26 TC 16 Z9 17 U1 4 U2 31 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2012 VL 62 IS 1 BP 1 EP 9 DI 10.1016/j.biocontrol.2012.03.006 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 945GY UT WOS:000304262300001 ER PT J AU Pusey, PL Wend, C AF Pusey, P. L. Wend, C. TI Potential of osmoadaptation for improving Pantoea agglomerans E325 as biocontrol agent for fire blight of apple and pear SO BIOLOGICAL CONTROL LA English DT Article DE Erwinia amylovora; Malus domestica; Trehalose; Xanthan gum ID ERWINIA-AMYLOVORA; BIOLOGICAL-CONTROL; GLYCINE BETAINE; STRAIN E325; BLOSSOMS; NECTAR; ANTAGONISTS; ANTIBIOSIS; WATER; ACCUMULATION AB Pantoea agglomerans biocontrol strain E325 is the active ingredient in a commercial product for fire blight, a destructive disease of apple and pear initiated by Erwinia amylovora in flowers. Osmoadaptation, involving the combination of saline osmotic stress and osmolyte amendment to growth media, was investigated to improve the epiphytic colonization by E325 on apple flowers, particularly in dry climates. E325 was osmoadapted in nutrient yeast dextrose broth and in the commercial fermentation medium, both amended with NaCl and glycine betaine according to previous research. The bacterium was cultured and freeze dried with cryoprotectants at an ARS-USDA laboratory (Wenatchee, WA) and a commercial facility (Pasco, WA) prior to treating apple flowers in an orchard where relative humidity (RH) averaged <50%. On orchard flowers and on detached crab apple flowers, osmoadaptation generally did not affect colonization of E325 on flower stigmas or nectar-rich hypanthia. The exception was the significant advantage of osmoadapted E325 on hypanthia of detached flowers at 70% RH, resulting in osmotic conditions marginally conducive for bacteria. Osmoadaptation proved most beneficial for increasing E325 survival during freeze drying and storage prior to orchard application. It also complemented cryoprotection, improving overall stability of freeze dried preparations of this biocontrol agent. Published by Elsevier Inc. C1 [Pusey, P. L.] ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Wend, C.] NW Agr Prod, Pasco, WA USA. RP Pusey, PL (reprint author), ARS, USDA, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. EM larry.pusey@tfrl.ars.usda FU USDA-CSREES-SCRI FX The authors thank Brenda Steady, Janet Duffy, Tara Culbertson, Lora Dunlap, Cindy Kahn, and Nancy Buchanan for technical assistance; Van Well Nursery for trees; and USDA-CSREES-SCRI for grant funds. NR 36 TC 4 Z9 4 U1 0 U2 13 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2012 VL 62 IS 1 BP 29 EP 37 DI 10.1016/j.biocontrol.2012.03.002 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 945GY UT WOS:000304262300005 ER PT J AU Beck, BH Fuller, SA Peatman, E McEntire, ME Darwish, A Freeman, DW AF Beck, Benjamin H. Fuller, S. Adam Peatman, Eric McEntire, Matthew E. Darwish, Ahmed Freeman, Donald W. TI Chronic exogenous kisspeptin administration accelerates gonadal development in basses of the genus Morone SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY LA English DT Article DE KISS1; KISS2; KISS1R; Kisspeptin; Puberty ID METASTASIS-SUPPRESSOR GENE; PROTEIN-COUPLED RECEPTOR; MEDAKA ORYZIAS-LATIPES; STRIPED BASS; SYSTEM; KISS-1; IDENTIFICATION; EXPRESSION; PUBERTY; CELLS AB The present study assesses the effects of chronic administration of peptides to fish, termed kisspeptins, which are the products of the KISS1 and KISS2 genes, and have been shown to control the development of puberty in animals. Using ecologically and commercially important species (white bass, Morone chrysops, striped bass, Morone saxatilis, and their hybrid) as comparative models, we determined that repeated bi-weekly injections (over 7 weeks) differentially accelerate puberty, as evidenced by increases in the prevalence of spermatozoa in the testes of juvenile fish. Moreover, in sexually mature fish, kisspeptin treatment led to increased gonad weight, gonadosomatic index, and spermatocrit in some white and striped bass. Additionally, mature white bass treated with kisspeptins showed an advancement in oocyte development as determined by histological examination. These gonadal changes occurred in the absence of any photothermal manipulation or hormone injections. To date, this is the first description of kisspeptin-mediated pubertal initiation in fish, and the first evidence that kisspeptins could modulate gonad maturation. Although it remains to be determined how kisspeptins may best be utilized in practice, our findings are a basis for future studies to characterize the molecular underpinnings of the KISS system in various fish species. Published by Elsevier Inc. C1 [Beck, Benjamin H.; Fuller, S. Adam; McEntire, Matthew E.; Darwish, Ahmed; Freeman, Donald W.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Peatman, Eric] Auburn Univ, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA. RP Beck, BH (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM benjamin.beck@ars.usda.gov FU USDA/ARS [6225-31630-006-00] FX The authors would like to thank Matt Barnett, Troy Bader, Bobby Kelly, and Jason Brown for their technical assistance and expertise. We would also like to thank Dave Straus, Thomas Bodenstine, and Huseyin Kucuktas for carefully reviewing the manuscript. This study was funded by the USDA/ARS under project number 6225-31630-006-00. The USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 44 TC 26 Z9 27 U1 0 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1095-6433 J9 COMP BIOCHEM PHYS A JI Comp. Biochem. Physiol. A-Mol. Integr. Physiol. PD JUL PY 2012 VL 162 IS 3 BP 265 EP 273 DI 10.1016/j.cbpa.2012.03.019 PG 9 WC Biochemistry & Molecular Biology; Physiology; Zoology SC Biochemistry & Molecular Biology; Physiology; Zoology GA 948NL UT WOS:000304509800015 PM 22497780 ER PT J AU Thomson, J AF Thomson, James TI Development of Site-specific Recombinase Technology for Precise Crop Plant Genome Modification SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Thomson, James] USDA, Western Reg Res Ctr, Crop Improvement & Utilizat Unit, Albany, CA 94710 USA. EM James.Thomson@ARS.USDA.GOV NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 22 EP 22 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400056 ER PT J AU Liu, ZR AF Liu, Zongrang TI Genetic and Epigenetic Beauty, Ugliness and Complexity of Flower-specific Promoters and Enhancers SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Liu, Zongrang] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. EM Zongrang.Liu@ARS.USDA.GOV NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 27 EP 27 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400072 ER PT J AU Thilmony, R Cook, M Guttman, ME AF Thilmony, R. Cook, M. Guttman, M. E. TI Leaf-, Root- and Pollen-specific Promoters from Rice; Useful New Tools for Improved Crop Biotechnology SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Thilmony, R.; Cook, M.; Guttman, M. E.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. EM roger.thilmony@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 27 EP 27 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400073 ER PT J AU Mall, TK Dweikat, I Sato, S Ge, ZX Nersian, N Chen, H Elthon, T Loerger, B Tilley, M Bean, S Clemente, T AF Mall, Tejinder Kumar Dweikat, Ismail Sato, Shirley Ge, Zhengxiang Nersian, Natalya Chen, Han Elthon, Tom Loerger, Brian Tilley, Mike Bean, Scott Clemente, Tom TI Improving End Use Functionality of Grain Sorghum SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Mall, Tejinder Kumar; Dweikat, Ismail; Elthon, Tom; Clemente, Tom] Univ Nebraska Lincoln, Dept Agron & Hort, Lincoln, NE USA. [Sato, Shirley; Ge, Zhengxiang; Nersian, Natalya; Chen, Han; Clemente, Tom] Univ Nebraska Lincoln, Ctr Biotechnol, Lincoln, NE USA. [Loerger, Brian; Tilley, Mike; Bean, Scott] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Clemente, Tom] Univ Nebraska Lincoln, Ctr Plant Sci Innovat, Lincoln, NE USA. EM tejinderkumar@rediffmail.com; tclemente1@unl.edu NR 0 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 37 EP 37 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400094 ER PT J AU Poothong, S Reed, BM AF Poothong, Sukalya Reed, Barbara M. TI MS Mineral Nutrients Limit Growth and Development of Micropropagated Red Raspberries SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Poothong, Sukalya] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM sukalya_p@hotmail.com; Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 38 EP 39 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400098 ER PT J AU Stevens, ME Pijut, PM AF Stevens, Micah E. Pijut, Paula M. TI Agrobacterium-mediated Transformation and Regeneration of Pumpkin Ash (Fraxinus profunda) Hypocotyls SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Stevens, Micah E.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. EM steven31@purdue.edu; ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 39 EP 40 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400100 ER PT J AU Payyavula, RS Navarre, DA AF Payyavula, R. S. Navarre, D. A. TI RNAi Mediated Silencing of Hydroxycinnamoyl-CoA Quinate-Hydroxycinnamoyl Transferase Alters Phenylpropanoid Metabolism in Potato SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Payyavula, R. S.; Navarre, D. A.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Navarre, D. A.] USDA ARS, Washington, DC 20250 USA. EM raja.payyavula@ars.usda.gov; navarrer@wsu.edu RI payyavula, raja/K-8078-2013 NR 0 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 59 EP 60 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400146 ER PT J AU Hand, C Reed, BM AF Hand, Charles Reed, Barbara M. TI Improving Mineral Nutrition of Micropropagated Hazelnuts SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Hand, Charles] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Reed, Barbara M.] USDA ARS Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. EM handc@onid.orst.edu; Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 62 EP 63 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400154 ER PT J AU Jenderek, MM Holman, GE Ellis, D Reed, BM AF Jenderek, M. M. Holman, G. E. Ellis, D. Reed, B. M. TI Long-term Preservation of Pycnanthemum Genetic Resources SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Jenderek, M. M.; Holman, G. E.; Ellis, D.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80521 USA. [Reed, B. M.] USDA ARS, Corvallis, OR 97333 USA. EM maria.jenderek@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 63 EP 63 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400156 ER PT J AU Rupp, JL Fellers, JP Trick, HN AF Rupp, Jessica L. Fellers, John P. Trick, Harold N. TI RNAi Mediated Viral Resistance in Transgenic Wheat: Stability Over Five Generations SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Rupp, Jessica L.; Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Fellers, John P.] ARS, USDA, Hard Winter Wheat Genet Res Unit, Ctr Grain & Anim Hlth Res Manhattan, Manhattan, KS 66506 USA. EM jrupp@ksu.edu NR 0 TC 0 Z9 0 U1 0 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 66 EP 67 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400164 ER PT J AU Wada, S Niedz, RP Evens, TJ Reed, BM AF Wada, Sugae Niedz, Randall P. Evens, Terrence J. Reed, Barbara M. TI Mesos Components Improve Pear Germplasm Micropropagation SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Wada, Sugae] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Niedz, Randall P.; Evens, Terrence J.] US Hort Res Lab, Hort & Breeding Unit, Ft Pierce, FL 34945 USA. [Reed, Barbara M.] USDA ARS, Corvallis, OR 97333 USA. EM wadas@hort.oregonstate.edu; Barbara.Reed@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 69 EP 69 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400171 ER PT J AU Dang, PM Chen, CY Lamb, MC AF Dang, P. M. Chen, C. Y. Lamb, M. C. TI Development of a Rapid Regeneration Method for Runner-type peanut (Arachis hypogaea L.). SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Dang, P. M.; Chen, C. Y.; Lamb, M. C.] ARS, USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA. EM phat.dang@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 72 EP 72 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400179 ER PT J AU Wang, Y Pijut, PM AF Wang, Y. Pijut, P. M. TI Isolation and Characterization of TERMINAL FLOWER 1 Homolog from Black Cherry (Prunus serotina Ehrh.) SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Wang, Y.] Purdue Univ, Dept Forestry & Nat Resources, HTIRC, W Lafayette, IN 47907 USA. [Pijut, P. M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. EM wang4@purdue.edu; ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 0 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 74 EP 75 PG 2 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400186 ER PT J AU Palla, KJ Pijut, PM AF Palla, Kaitlin J. Pijut, Paula M. TI Agrobacterium-mediated Transformation of Fraxinus americana L. Hypocotyls SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL LA English DT Meeting Abstract C1 [Palla, Kaitlin J.] Purdue Univ, Dept Forestry & Nat Resources, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. EM kpalla@purdue.edu; ppijut@purdue.edu RI Pijut, Paula/N-6789-2015 NR 0 TC 0 Z9 0 U1 0 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1071-2690 J9 IN VITRO CELL DEV-AN JI In Vitro Cell. Dev. Biol.-Anim. PD JUL PY 2012 VL 48 SU 1 BP 81 EP 81 PG 1 WC Cell Biology; Developmental Biology SC Cell Biology; Developmental Biology GA 947BU UT WOS:000304402400204 ER PT J AU Hourdequin, M Landres, P Hanson, MJ Craig, DR AF Hourdequin, Marion Landres, Peter Hanson, Mark J. Craig, David R. TI Ethical implications of democratic theory for U.S. public participation in environmental impact assessment SO ENVIRONMENTAL IMPACT ASSESSMENT REVIEW LA English DT Article DE Ethics; Democratic theory; Public participation; NEPA; Collaboration ID RESOURCE DECISION-MAKING; COLLABORATIVE ECOSYSTEM MANAGEMENT; CITIZEN PARTICIPATION; NATURAL-RESOURCES; FOREST-SERVICE; INVOLVEMENT; FAIRNESS; PERSPECTIVES; COMPETENCE; GOVERNANCE AB Traditional mechanisms for public participation in environmental impact assessment under U.S. federal law have been criticized as ineffective and unable to resolve conflict. As these mechanisms are modified and new approaches developed, we argue that participation should be designed and evaluated not only on practical grounds of cost-effectiveness and efficiency, but also on ethical grounds based on democratic ideals. In this paper, we review and synthesize modern democratic theory to develop and justify four ethical principles for public participation: equal opportunity to participate, equal access to information, genuine deliberation, and shared commitment. We then explore several tensions that are inherent in applying these ethical principles to public participation in EIA. We next examine traditional NEPA processes and newer collaborative approaches in light of these principles. Finally, we explore the circumstances that argue for more in-depth participatory processes. While improved EIA participatory processes do not guarantee improved outcomes in environmental management, processes informed by these four ethical principles derived from democratic theory may lead to increased public engagement and satisfaction with govemment agency decisions. (C) 2012 Elsevier Inc. All rights reserved. C1 [Hourdequin, Marion] Colorado Coll, Dept Philosophy, Colorado Springs, CO 80903 USA. [Hourdequin, Marion; Landres, Peter; Craig, David R.] USDA Forest Serv, Rocky Mt Res Stn, Aldo Leopold Wilderness Res Inst, Missoula, MT 59801 USA. [Hanson, Mark J.] Univ Montana, Dept Philosophy, Missoula, MT 59812 USA. RP Hourdequin, M (reprint author), Colorado Coll, Dept Philosophy, 14 E Cache La Poudre St, Colorado Springs, CO 80903 USA. EM Marion.Hourdequin@ColoradoCollege.edu NR 81 TC 4 Z9 6 U1 4 U2 56 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0195-9255 J9 ENVIRON IMPACT ASSES JI Environ. Impact Assess. Rev. PD JUL PY 2012 VL 35 BP 37 EP 44 DI 10.1016/j.eiar.2012.02.001 PG 8 WC Environmental Studies SC Environmental Sciences & Ecology GA 944PX UT WOS:000304217400004 ER PT J AU Samuels, GJ Ismaiel, A Mulaw, TB Szakacs, G Druzhinina, IS Kubicek, CP Jaklitsch, WM AF Samuels, Gary J. Ismaiel, Adnan Mulaw, Temesgen B. Szakacs, George Druzhinina, Irina S. Kubicek, Christian P. Jaklitsch, Walter M. TI The Longibrachiatum Clade of Trichoderma: a revision with new species SO FUNGAL DIVERSITY LA English DT Article DE Hypocrea; Hypocreaceae; Hypocreales; Endophyte; Medical mycology; Revision; Systematics; Taxonomy; Soil fungi; Biogeography ID GENUS TRICHODERMA; HYPOCREA-JECORINA; SECT LONGIBRACHIATUM; CELLULASE PRODUCTION; REESEI; IDENTIFICATION; DIVERSITY; STRAINS; STRATEGIES; FUNGI AB The Longibrachiatum Clade of Trichoderma is revised. Eight new species are described (T. aethiopicum, T. capillare, T. flagellatum, T. gillesii, T. gracile, T. pinnatum, T. saturnisporopsis, T. solani). The twenty-one species known to belong to the Longibrachiatum Clade are included in a synoptic key. and are redescribed based on new collections or additional observations. teleomorphs are reported for and . Previously described species are annotated. C1 [Jaklitsch, Walter M.] Univ Vienna, Fac Ctr Biodivers, Dept Systemat & Evolutionary Bot, A-1030 Vienna, Austria. [Samuels, Gary J.; Ismaiel, Adnan] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD 20705 USA. [Mulaw, Temesgen B.] Univ Maryland, Inst Marine & Environm Technol, Ctr Environm Sci, Baltimore, MD 21201 USA. [Szakacs, George] Budapest Univ Technol & Econ BUTE, Dept Appl Biotechnol & Food Sci, H-1111 Budapest, Hungary. [Druzhinina, Irina S.; Kubicek, Christian P.] Vienna Univ Technol, Inst Chem Engn, A-1060 Vienna, Austria. RP Jaklitsch, WM (reprint author), Univ Vienna, Fac Ctr Biodivers, Dept Systemat & Evolutionary Bot, Rennweg 14, A-1030 Vienna, Austria. EM walter.jaklitsch@univie.ac.at RI Szakacs, George/H-7233-2012; Jaklitsch, Walter/B-4970-2013 FU NSF [DEB 0089474]; Austrian Science Foundation [FWF P-19340-MOB]; Austrian Science Fund (FWF) [P22081-B17] FX Over several years cultures for this project were provided by Toru Okuda (formerly Nippon Roche) Japan; Giovanni Vanacci, University of Pisa; Harry Evans, CABI UK; Le Dinh Don, Long Nam University, Vietnam; Enrique Arevalo, ICT Peru; Andrews Akrofi, CRIG, Ghana; Sunday Agbeniyi, CRIN, Nigeria; Pierre Tondje, IRAD, Cameroon; G. Gilles and Francoise Candoussau, Pau, France; V. Doyle, The New York Botanical Garden, and V. S. Lopez, Universidad del Papaloapan, Oaxaca, Mexico; Tomas Melgarejo, Universidad Nacional Agrararia La Molina, Lima, Peru. Orlando Petrini corrected several of the Latin descriptions. Collecting in Sri Lanka was supported by NSF grant DEB 0089474 to the Dept. of Plant Pathology, The Pennsylvania State University. Work in the lab of C. P. K. was supported by the Austrian Science Foundation (grant FWF P-19340-MOB). The financial support of W.M.J. by the Austrian Science Fund (FWF; project P22081-B17) is acknowledged. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. The U. S. Department of Agriculture is an equal opportunity employer. NR 50 TC 31 Z9 35 U1 1 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1560-2745 J9 FUNGAL DIVERS JI Fungal Divers. PD JUL PY 2012 VL 55 IS 1 BP 77 EP 108 DI 10.1007/s13225-012-0152-2 PG 32 WC Mycology SC Mycology GA 943RF UT WOS:000304142200005 PM 22956918 ER PT J AU Nakasone, KK Lindner, DL AF Nakasone, Karen K. Lindner, Daniel L. TI Taxonomy of Pseudolagarobasidium (Polyporales, Basidiomycota) SO FUNGAL DIVERSITY LA English DT Article DE Pirex concentricum; Pseudolagarobasidium; acaciicola; P. leguminicola; Hydnum ayresii; Microbinding hyphae ID CORTICIOID FUNGI; GENUS; APHYLLOPHORALES; CORTICIACEAE; AGARICALES; GENERA AB Pseudolagarobasidium (Polyporales, Basidiomycota) is a small, monophyletic genus of crustose, wood-inhabiting fungi with spines and a saprobic, endophytic, or parasitic habit. Seven species are accepted in the genus including two new species, P. belizense from Belize and P. pusillum from Australia. Sequence analysis of the internal transcribed spacer of the ribosomal RNA gene places P. belizense in a monophyletic clade with P. acaciicola and an undescribed foliar endophyte. New combinations proposed include P. modestum for Irpex modestus Berk., P. pronum for Hydnum pronum Berk. & Broome which is an earlier name for P. calcareum, and P. venustum for Radulodon venustus Hjortstam & Ryvarden. Irpex colliculosum Berk. & Broome from Sri Lanka is conspecific with P. subvinosum. Two species, Sistotrema ochroleucum and Radulum concentricum are not accepted in Pseudolagarobasidium. Pseudolagarobasidium is compared with Radulodon and similar genera. A key to the species of Pseudolagarobasidium is provided. C1 [Nakasone, Karen K.; Lindner, Daniel L.] US Forest Serv, Ctr Forest Mycol Res, No Res Stn, Madison, WI 53726 USA. RP Nakasone, KK (reprint author), US Forest Serv, Ctr Forest Mycol Res, No Res Stn, 1 Gifford Pinchot Dr, Madison, WI 53726 USA. EM knakasone@fs.fed.us FU National Science Foundation; Biodiversity Surveys and Inventories Program; State University of New York, College at Cortland [DEB-0103621]; Center for Forest Mycology Research, Northern Research Station, U.S. Forest Service FX We thank the curators of BPI, H, K, NY, O, PERTH, PREM, S, and TFM for their assistance in arranging specimen loans. Kyah Norton assisted in obtaining DNA sequences of P. belizense. Fieldwork in Belize was made possible by a grant from the National Science Foundation, Biodiversity Surveys and Inventories Program to Dr. T.J. Baroni, the State University of New York, College at Cortland (DEB-0103621), in collaboration with Dr. D.J. Lodge and the Center for Forest Mycology Research, Northern Research Station, U.S. Forest Service. Dr. Lodge helped coordinate logistical support for the field work in Belize, and the British Forces of Belize provided airlift transportation to Doyle's Delight Peak in the Maya Mountains. Alan Whitelaw, the commanding officer of the Belize Forces, and Sharon Matola, Director of the Belize Zoo, were responsible for making the expeditions possible. Several other individuals with government and nongovernmental agencies in Belize are kindly acknowledged for their help and guidance: Hector Mai, John Pinelo, and Natalie Rosado of The Conservation Division, Belize Forestry Department, Belmopan; and Celso Poot, Station Manager of the Tropical Education Center in Belize. Drs. S. P. Gorjon and J. Stalpers reviewed an earlier draft of the manuscript NR 48 TC 3 Z9 3 U1 0 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1560-2745 J9 FUNGAL DIVERS JI Fungal Divers. PD JUL PY 2012 VL 55 IS 1 BP 155 EP 169 DI 10.1007/s13225-012-0161-1 PG 15 WC Mycology SC Mycology GA 943RF UT WOS:000304142200009 ER PT J AU Booth, DT Cox, SE Simonds, G Sant, ED AF Booth, D. Terrance Cox, Samuel E. Simonds, Gregg Sant, Eric D. TI Willow cover as a stream-recovery indicator under a conservation grazing plan SO ECOLOGICAL INDICATORS LA English DT Article DE Aerial imagery; Effectiveness monitoring; Ecological indicators; Riparian management; Objective measurements; Watershed-scale sampling ID MAPPING RIPARIAN VEGETATION; TROUT POPULATIONS; DIGITAL IMAGERY; CUTTHROAT TROUT; WILD UNGULATE; LIVESTOCK; COMMUNITIES; RETENTION; ECOSYSTEM; HABITATS AB Many rangeland streams and associated fisheries have suffered from livestock grazing as a cost of upland-forage utilization. Due to damage from intensive usage, restoration of damaged streams is now a common land-management objective. The Squaw Valley Ranch of Elko County, Nevada, US, in cooperation with the US Bureau of Land Management (BLM) and Barrick Gold Corp., is attempting to improve those portions of the Rock Creek watershed negatively affected by past ranch operations. The watershed includes both historical and occupied habitat for the threatened Lahonton cutthroat trout (Oncorhynchus clarki henshawi [Richardson]). From 2003, and continuing to the present, hot-season livestock grazing on Squaw Valley Ranch private and permitted public-land riparian areas was greatly reduced. To assess the effectiveness of this conservation effort, we (1) evaluated BLM archived images of riparian photo points in the watershed, (2) tested for change over time using data from systematic, intermittent, aerial sampling that acquired 2-cm resolution images from low-altitude surveys conducted in 2003, 2004 and 2006, and (3) compared Landsat scenes of the area from before and after 2003. Willow (Salix spp.) cover was chosen as the primary ecological indictor of riparian condition and we introduce willow canopy (m(2)) per m of stream length in the image field-of-view, as a practical measure of willow status. Archived images from photo points show mostly low-condition riparian plant communities, often with little or no willow canopy evident before 2003, but with conspicuous improvement thereafter. This subjective perception is supported by objective analyses finding, (1) the relative increase in willow cover nearly tripled on one stream, more than doubled on three others, and increased on all but one (fire affected) and (2) a highly significant post-2003 increase in willows in the Landsat record. Thus, the post-2003 increase in willow cover documented in three complementary lines of evidence from ground, air, and space support the predicted ecological benefits of reduced hot-season riparian grazing and the utility of 2-cm imagery as a tool for assessing watershed-wide conservation benefits from a federal cost-share-eligible conservation practice. This appears to be the first use of willow measurements from an aerial survey as a particular indicator of riparian condition and trend and the first demonstration of change detection based on objective measurements from a watershed-scale riparian monitoring effort that used systematic sampling (versus subjective selection) and high sample density to address the large Type II error (false negative) risk common to conventional land-management survey efforts. Published by Elsevier Ltd. C1 [Booth, D. Terrance; Cox, Samuel E.] ARS, USDA, High Plains Grassland Res Stn, Cheyenne, WY 82009 USA. [Simonds, Gregg; Sant, Eric D.] Open Range Consulting, Park City, UT 84098 USA. RP Booth, DT (reprint author), ARS, USDA, High Plains Grassland Res Stn, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. EM Terry.Booth@ars.usda.gov RI Girard, Carlin/G-6652-2012 FU Barrick Gold Corporation (Barrick), Elko, Nevada; USDA-ARS; Wyoming State Office of the BLM FX The research was funded in part by Barrick Gold Corporation (Barrick), Elko, Nevada; the USDA-ARS; and the Wyoming State Office of the BLM. Carol Evans, Fisheries Biologist for BLM, Elko Field Office, provided valuable assistance. Barrick provided aviation facilities and crew lodging. Joe Nance, CloudStreet Flying Services, acquired aerial images. Carmen Kennedy, USDA-ARS, provided technical assistance. Dr. Phil Chapman, Colorado State University Department of Statistics, consulted with us on the statistical analyses, and Larry Griffith, USDA-ARS assisted in the analysis. We thank Drs. G. Perry and D. W. Bailey, also C. Evans and M. Bishop for reviewing an earlier version of the manuscript. Throughout this manuscript, mention of products and proprietary names is for information only and does not constitute an endorsement by the authors, Barrick, USDA, or BLM. NR 47 TC 6 Z9 5 U1 1 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1470-160X J9 ECOL INDIC JI Ecol. Indic. PD JUL PY 2012 VL 18 BP 512 EP 519 DI 10.1016/j.ecolind.2011.12.017 PG 8 WC Biodiversity Conservation; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 932IQ UT WOS:000303284500056 ER PT J AU Stout, JE AF Stout, J. E. TI A field study of wind erosion following a grass fire on the Llano Estacado of North America SO JOURNAL OF ARID ENVIRONMENTS LA English DT Article DE Aeolian; Blown sand; Grassland; Saltation; Wildfire ID SOUTHERN HIGH-PLAINS; TRANSPORT AB Interactions between earth, wind, and fire have always played an important role in the formation and evolution of the level plains of the Llano Estacado of North America. The uppermost sediments of this vast region are aeolian deposits, formed by aeolian deposition into grassland vegetation. Grass cover enhances aeolian deposition by slowing near-surface winds and vegetation secures sediments once they are deposited. The benefits of grass cover, however, are lost when occasional fires remove protective vegetation from fields. After a fire, the underlying soil surface becomes exposed and susceptible to wind erosion until the vegetative cover is re-established. The purpose of this study was to explore the post-fire recovery process by monitoring temporal variations in aeolian transport and changes in the threshold velocity of a burned grass field located in Lubbock County, Texas. A continuous record of wind erosion activity was obtained during a six-month period as the surface recovered from a highly erodible state to a more vegetated and stable surface. Results suggest that the threshold wind speed of the field increased from less than 10 m/s immediately following the fire to above 19 m/s in a three-month period as vegetation naturally recovered. Published by Elsevier Ltd. C1 USDA ARS, Lubbock, TX 79415 USA. RP Stout, JE (reprint author), USDA ARS, 3810 4th St, Lubbock, TX 79415 USA. EM john.stout@ars.usda.gov NR 20 TC 5 Z9 6 U1 0 U2 8 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0140-1963 J9 J ARID ENVIRON JI J. Arid. Environ. PD JUL PY 2012 VL 82 BP 165 EP 174 DI 10.1016/j.jaridenv2012.02.001 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 935VE UT WOS:000303549500019 ER PT J AU Geveke, DJ Torres, D AF Geveke, David J. Torres, Daniel TI Pasteurization of grapefruit juice using a centrifugal ultraviolet light irradiator SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Nonthermal; Pasteurization; Grapefruit juice; Escherichia coli; Saccharomyces cerevisiae; Ultraviolet light ID LIQUID EGG-WHITE; APPLE CIDER; ESCHERICHIA-COLI; UV INACTIVATION; ELECTRIC-FIELDS AB Studies are lacking on the nonthermal pasteurization of liquid foods using UV irradiators that centrifugally form very thin films to overcome the problem of limited penetration depth of UV. Grapefruit juice inoculated with Escherichia coli or Saccharomyces cerevisiae was processed at the following conditions: UV dose 4.8-24 mJ/cm(2); treatment time 3.2 s, cylinder rotational speed 450-750 rpm, cylinder inclination angle 15-45 degrees, outlet temperature 11 degrees C, and flow rate 300 ml/min, and was stored for 35 days. Appropriate dilutions of the samples were pour plated with TSA and TSA + 3% NaCl for E. coli and Sabouraud dextrose agar (SDA) and SDA + 5% NaCl for S. cerevisiae. Nonthermal UV processing at 19 mJ/cm(2), 450 rpm and 15 degrees reduced E. coli in grapefruit juice by 5.1 log(10). A dose of 14 mJ/cm(2) reduced S. cerevisiae by 6.0 log(10). Inactivation increased linearly with increasing UV dose. The inactivations at 600 and 750 rpm were similar, and were better than at 450 rpm. The results at 30 degrees and 45 degrees were similar, and were better than at 15 degrees. The occurrence of sublethal injury in either microorganism was not detected. Storing UV processed grapefruit juice at 4 and 10 degrees C reduced the surviving E. coli to below 1 log(10) cfu/ml in 14 days. Processing UV juice reduced the population of S. cerevisiae to less than 1 log(10) cfu/ml where it remained for 35 days during refrigerated storage. These results suggest that grapefruit juice may be pasteurized using a nonthermal UV irradiator that centrifugally forms a thin film. Published by Elsevier Ltd. C1 [Geveke, David J.] ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Torres, Daniel] Dill Instruments Inc, Kalamazoo, MI 49001 USA. RP Geveke, DJ (reprint author), ARS, Food Safety & Intervent Technol Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM david.geveke@ars.usda.gov NR 24 TC 7 Z9 7 U1 1 U2 17 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD JUL PY 2012 VL 111 IS 2 BP 241 EP 246 DI 10.1016/j.jfoodeng.2012.02.026 PG 6 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 932JB UT WOS:000303286200006 ER PT J AU Sayaslan, A Seib, PA Chung, OK AF Sayaslan, Abdulvahit Seib, Paul A. Chung, Okkyung Kim TI A bench-scale high-shear wet-milling test for wheat flour SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Wheat flour; Wet-milling quality; High-shear test ID WATER UNEXTRACTABLE SOLIDS; TRITICUM-AESTIVUM L.; DOUGH PROPERTIES; GLUTEN PROTEIN; BATTER SYSTEM; STARCH; SEPARATION; FRACTIONATION; QUALITY AB Bench-scale wet-milling tests for wheat flour are available for the traditional processes of Martin and Batter, but tests for the high-shear processes of Alfa-Laval/Raisio, Hydrocyclone, and High-Pressure Disintegration are few in number. In this study, critical processing parameters of a high-shear wet-milling process, namely high-shear mixing, gluten-aging, and gluten-washing steps, were investigated using response surface methodology, and those parameters led to a bench-scale wet-milling test starting with a "highly sheared flour-water dispersion" (HS-FWD). Optimum conditions for the test were: a water-flour ratio (db) of 1.7, water temperature of 35 degrees C, and homogenizer speed of 6000 rpm for 2.0 min in the high-shear mixing step, a temperature of 40 degrees C for 20 min in the gluten-aging step, and gluten-washing of 2.0 min in the Glutomatic system. The HS-FWD wet-milling test with high level of repeatability was capable of discriminating wet-milling qualities of several hard, soft, and coarsely ground wheat flours. (c) 2012 Elsevier Ltd. All rights reserved. C1 [Sayaslan, Abdulvahit] Karamanoglu Mehmetbey Univ, Dept Food Engn, Karaman, Turkey. [Seib, Paul A.] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Chung, Okkyung Kim] USDA ARS, Grain Mkt & Prod Res Ctr, Manhattan, KS USA. RP Sayaslan, A (reprint author), Karamanoglu Mehmetbey Univ, Dept Food Engn, Karaman, Turkey. EM sayaslan@kmu.edu.tr NR 47 TC 2 Z9 2 U1 2 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 J9 J FOOD ENG JI J. Food Eng. PD JUL PY 2012 VL 111 IS 2 BP 305 EP 317 DI 10.1016/j.jfoodeng.2012.02.018 PG 13 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 932JB UT WOS:000303286200014 ER PT J AU Burdick, NC Carroll, JA Dailey, JW Randel, RD Falkenberg, SM Schmidt, TB AF Burdick, N. C. Carroll, J. A. Dailey, J. W. Randel, R. D. Falkenberg, S. M. Schmidt, T. B. TI Development of a self-contained, indwelling vaginal temperature probe for use in cattle research SO JOURNAL OF THERMAL BIOLOGY LA English DT Article DE Cattle; Indwelling temperature probe; Rectal temperature monitoring; Vaginal temperature monitoring ID BODY-TEMPERATURE; RECTAL TEMPERATURE; FEEDLOT CATTLE; TECHNICAL-NOTE; DAIRY-COWS; LIPOPOLYSACCHARIDE CHALLENGE; BEEF STEERS; TEMPERAMENT; ENDOTOXIN; RESPONSES AB A device was developed to monitor the vaginal temperature of cattle in a research setting. This device decreases labor involved with monitoring body temperature compared with manual temperature readings, allows for continuous monitoring of vaginal temperature at 1 min intervals, and also allows for temperature measurements without the presence of a human handler or without restraint, which can agitate cattle. The device consists of a blank controlled internal drug release (CIDR) device (designed by Pfizer Animal Health as an indwelling vaginal probe) that holds an indwelling vaginal temperature probe logger. The fabrication of the vaginal probe costs approximately US $325 per unit. Similar rectal and vaginal temperature responses to lipopolysaccharide challenge were observed when vaginal and rectal temperatures were measured simultaneously in the same heifer (P > 0.05). Additionally, rectal and vaginal temperatures were highly correlated (r = 0.97; P < 0.0001). Similar to the rectal temperature monitoring device, the vaginal device allows for the measurement of vaginal temperature without the potential biases associated with the stress response produced as a reaction to the handling by and (or) presence of humans. The vaginal temperature recording device will provide researchers with an additional inexpensive tool to study physiological responses in female cattle. Published by Elsevier Ltd. C1 [Burdick, N. C.; Carroll, J. A.; Dailey, J. W.] USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. [Randel, R. D.] Texas A&M Syst, Texas AgriLife Res, Overton, TX 75684 USA. [Falkenberg, S. M.; Schmidt, T. B.] Mississippi State Univ, Dept Anim & Dairy Sci, Mississippi State, MS 39762 USA. RP Carroll, JA (reprint author), USDA ARS, Livestock Issues Res Unit, Lubbock, TX 79403 USA. EM Jeff.Carroll@ars.usda.gov NR 31 TC 9 Z9 10 U1 0 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0306-4565 J9 J THERM BIOL JI J. Therm. Biol. PD JUL PY 2012 VL 37 IS 4 SI SI BP 339 EP 343 DI 10.1016/j.jtherbio.2011.10.007 PG 5 WC Biology; Zoology SC Life Sciences & Biomedicine - Other Topics; Zoology GA 932JC UT WOS:000303286300016 ER PT J AU Wise, J Vietor, D Provin, T Capareda, S Munster, C Boateng, A AF Wise, Jatara Vietor, Donald Provin, Tony Capareda, Sergio Munster, Clyde Boateng, Akwasi TI Mineral Nutrient Recovery from Pyrolysis Systems SO ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY LA English DT Article ID AEROSOL-PARTICLES; BIOMASS; BIOCHAR; QUALITY AB Bioenergy plants such as sorghum, bioenergy rice, corn stover, and switchgrass can be thermochemically converted by pyrolysis to produce bio-oil, synthesis gas from noncondensable gases, and biochar. The biochar fraction can be recycled back to the production field to improve soil physical qualities and nutrient status. Although previous publications have described the beneficial effects of pyrolysis biochar on soil physical properties; relatively little has been published on the recovery of mineral nutrients from pyrolysis co-products. This work quantified the recovery of nutrients (P, K, Ca, and Mg) from pyrolysis coproducts from various feedstocks using two distinct reactors. Nutrient mass balances, on a feedstock basis, were calculated for comparison of the two reactors' efficiency in the recovery of the nutrients. The results revealed the recovery of nutrients varied by (1) species, (2) reactor design, and (3) correlated highly with nutrient mass loss in biochar. Computations also revealed P recoveries of 93% (fixed-bed reactor) and 58% and 55% (fluidized-bed reactor) for pyrolyzed sorghum. The recovery of key mineral nutrients in pyrolysis coproducts (primarily biochar) is directly related to the feasibility of nutrient recycling through biochar. (C) 2012 American Institute of Chemical Engineers Environ Prog, 31: 251-255, 2012 C1 [Wise, Jatara; Vietor, Donald; Provin, Tony; Capareda, Sergio; Munster, Clyde] Texas A&M Univ, College Stn, TX 77843 USA. [Boateng, Akwasi] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA USA. RP Wise, J (reprint author), Texas A&M Univ, College Stn, TX 77843 USA. EM jatarawise@gmail.com FU United States Department of Agriculture; Department of Energy; Hispanic Leaders in Agriculture; Environment (HLAE) at Texas AM University; Alfred P. Sloan Foundation FX Fellowship support of Jatara Wise was provided from the United States Department of Agriculture National Needs Fellowship in Bioenergy. In addition, partial support was provided the Department of Energy North Central SunGrant Program, the Alfred P. Sloan Foundation Minority PhD program, and the Hispanic Leaders in Agriculture and the Environment (HLAE) program at Texas A&M University. NR 16 TC 3 Z9 3 U1 0 U2 10 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 1944-7442 J9 ENVIRON PROG SUSTAIN JI Environ. Prog. Sustain. Energy PD JUL PY 2012 VL 31 IS 2 SI SI BP 251 EP 255 DI 10.1002/ep.11631 PG 5 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Environmental; Engineering, Chemical; Engineering, Industrial; Environmental Sciences SC Science & Technology - Other Topics; Engineering; Environmental Sciences & Ecology GA 925WY UT WOS:000302794000013 ER PT J AU Momany, FA Schnupf, U AF Momany, Frank A. Schnupf, Udo TI DFTr Studies of Five- and Six-Residue Cyclic-beta(1 -> 4) Cellulosic Molecules SO BIOPOLYMERS LA English DT Article DE density functional; 6-31+G*; 4-31G; cyclic cellulosic; synergistic ID CELLOBIOSE; INITIO; B3LYP/6-311++G; CONFORMATIONS AB Density functional (DFT) conformational in vacuo studies of cellobiose have shown that ?H-anti conformations are low in energy relative to the syn forms, while the ?H-anti forms are higher in energy. Further, as the cellulosic fragments became larger than a disaccharide and new hydrogen bonding interactions between multiple residues become available, stable low energy ?H-anti, and ?H-anti cellulosic structures became possible. To test the stability of cyclic anti-conformations, a number of beta-linked five- and six-residue molecules were created and then energy optimized in solvent (water, n-heptane) using the implicit solvation method COSMO at the B3LYP level of theory. The created symmetric cyclic structures were without distortion. Upon optimization some cyclic conformations were found to be of low energy when compared with linear five- and six-residue chains, after correcting the energy for the exclusion of a water molecule upon cyclization. It was also obvious from the hydrogen bonding network formed above and below the plane of the cyclic structure that these structures could exhibit strong synergistic tendencies. The conformational energy preferences for clockwise c and counter-clockwise r hydroxyl groups and preference for the hydroxymethyl rotamers is described. Because these structures contain energetically unfavorable flipped conformations in water, that is, dihedral angles of similar to 180 degrees/0 degrees or similar to 0 degrees/180 degrees in ?H/?H, it is clear that the synthesis of these compounds will be challenging. (c) 2012 Wiley Periodicals, Inc. Biopolymers 97:568576, 2012. C1 [Momany, Frank A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Schnupf, Udo] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. RP Momany, FA (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM frank.momany@ars.usda.gov RI Schnupf, Udo/H-4703-2016 OI Schnupf, Udo/0000-0002-1457-1985 NR 18 TC 1 Z9 1 U1 1 U2 7 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0006-3525 J9 BIOPOLYMERS JI Biopolymers PD JUL PY 2012 VL 97 IS 7 BP 568 EP 576 DI 10.1002/bip.22039 PG 9 WC Biochemistry & Molecular Biology; Biophysics SC Biochemistry & Molecular Biology; Biophysics GA 923FH UT WOS:000302604300009 PM 22354541 ER PT J AU Gonzalez, VH Griswold, TL AF Gonzalez, Victor H. Griswold, Terry L. TI NEW SPECIES AND PREVIOUSLY UNKNOWN MALES OF NEOTROPICAL CLEPTOBIOTIC STINGLESS BEES (HYMENOPTERA, APIDAE, LESTRIMELITTA) SO CALDASIA LA English DT Article DE Anthophila; Apoidea; Meliponini; Colombia; Venezuela; taxonomy ID SOUTH-AMERICA HYMENOPTERA; HALICTIDAE; COLOMBIA; GENUS; REVISION; COLLETIDAE; CHLEROGAS; MOURE; N. AB Three new species of cleptobiotic stingless bees of the genus Lestrimelitta Friese, L. opita sp. n. and L. huilensis sp. n. from Colombia and L. catira sp. n. from Venezuela, are described and figured. The males of the Central American species L. chamelensis, L. danuncia, and L. mourei are also described and figured. New geographical records and an updated key to species from Central America and northern South America are provided. C1 [Gonzalez, Victor H.] Univ Kansas, Nat Hist Museum, Div Entomol, Lawrence, KS 66045 USA. [Griswold, Terry L.] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. RP Gonzalez, VH (reprint author), Univ Kansas, Nat Hist Museum, Div Entomol, 1501 Crestline Dr,Suite 140, Lawrence, KS 66045 USA. EM victorgonzab@gmail.com; terry.griswold@ars.usda.gov RI Gonzalez, Victor/B-4072-2015 FU US National Science Foundation [DBI-1057366] FX We are grateful to B. Harris and S. Brady (USNM) and C. Sarmiento (ICN) for bringing this material to our attention and permitting its study; Orlando Rangel for the invitation to contribute to this volume; Michael S. Engel, Amy Comfort de Gonzalez, Molly Rightmyer, and anonymous reviewers for their comments and suggestions that improved this manuscript. Support to V.H.G. was provided by US National Science Foundation grant DBI-1057366 (to Michael S. Engel). This is a contribution of the Division of Entomology, University of Kansas Natural History Museum. NR 36 TC 2 Z9 2 U1 0 U2 1 PU INST CIENCIAS NATURALES, MUSEO HISTORIA NATURAL PI BOGOTA PA FAC CIENCIAS, UNIV NACIONAL COLOMBIA, APARTADO 7495, BOGOTA, 00000, COLOMBIA SN 0366-5232 J9 CALDASIA JI Caldasia PD JUN 30 PY 2012 VL 34 IS 1 BP 227 EP 245 PG 19 WC Plant Sciences; Multidisciplinary Sciences; Zoology SC Plant Sciences; Science & Technology - Other Topics; Zoology GA 089JQ UT WOS:000314907200016 ER PT J AU Gobster, PH Xiang, WN AF Gobster, Paul H. Xiang, Wei-Ning TI A revised aims and scope for Landscape and Urban Planning: An International Journal of Landscape Science, Planning and Design SO LANDSCAPE AND URBAN PLANNING LA English DT Editorial Material ID SPECIAL-ISSUE; ECOLOGY C1 [Gobster, Paul H.] US Forest Serv, USDA, No Res Stn, Evanston, IL 60201 USA. [Xiang, Wei-Ning] E China Normal Univ, Shanghai Inst Urban Ecol & Sustainabil, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Shanghai 200062, Peoples R China. E China Normal Univ, Shanghai 200062, Peoples R China. RP Gobster, PH (reprint author), US Forest Serv, USDA, No Res Stn, 1033 Univ Pl,Suite 360, Evanston, IL 60201 USA. EM pgobster@gmail.com; wnxiang@mail.ecnu.edu.cn RI Gobster, Paul/A-2826-2013 OI Gobster, Paul/0000-0002-8576-0310 NR 34 TC 7 Z9 7 U1 2 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD JUN 30 PY 2012 VL 106 IS 4 BP 289 EP 292 DI 10.1016/j.landurbplan.2012.05.010 PG 4 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 964UV UT WOS:000305723100001 ER PT J AU Cornman, RS Bennett, AK Murray, KD Evans, JD Elsik, CG Aronstein, K AF Cornman, R. Scott Bennett, Anna K. Murray, K. Daniel Evans, Jay D. Elsik, Christine G. Aronstein, Kate TI Transcriptome analysis of the honey bee fungal pathogen, Ascosphaera apis: implications for host pathogenesis SO BMC GENOMICS LA English DT Article ID MATING-TYPE LOCUS; ASPERGILLUS-NIDULANS; NEUROSPORA-CRASSA; GENE; SEQUENCE; MODEL; STERIGMATOCYSTIN; FUMIGATUS; ALIGNMENT; GENOMES AB Background: We present a comprehensive transcriptome analysis of the fungus Ascosphaera apis, an economically important pathogen of the Western honey bee (Apis mellifera) that causes chalkbrood disease. Our goals were to further annotate the A. apis reference genome and to identify genes that are candidates for being differentially expressed during host infection versus axenic culture. Results: We compared A. apis transcriptome sequence from mycelia grown on liquid or solid media with that dissected from host-infected tissue. 454 pyrosequencing provided 252 Mb of filtered sequence reads from both culture types that were assembled into 10,087 contigs. Transcript contigs, protein sequences from multiple fungal species, and ab initio gene predictions were included as evidence sources in the Maker gene prediction pipeline, resulting in 6,992 consensus gene models. A phylogeny based on 12 of these protein-coding loci further supported the taxonomic placement of Ascosphaera as sister to the core Onygenales. Several common protein domains were less abundant in A. apis compared with related ascomycete genomes, particularly cytochrome p450 and protein kinase domains. A novel gene family was identified that has expanded in some ascomycete lineages, but not others. We manually annotated genes with homologs in other fungal genomes that have known relevance to fungal virulence and life history. Functional categories of interest included genes involved in mating-type specification, intracellular signal transduction, and stress response. Computational and manual annotations have been made publicly available on the Bee Pests and Pathogens website. Conclusions: This comprehensive transcriptome analysis substantially enhances our understanding of the A. apis genome and its expression during infection of honey bee larvae. It also provides resources for future molecular studies of chalkbrood disease and ultimately improved disease management. C1 [Cornman, R. Scott; Murray, K. Daniel; Aronstein, Kate] USDA ARS, Honey Bee Res Unit, Weslaco, TX 78596 USA. [Evans, Jay D.] USDA ARS, Honey Bee Res Lab, Beltsville, MD 20705 USA. [Bennett, Anna K.; Elsik, Christine G.] Georgetown Univ, Dept Biol, Washington, DC 20057 USA. RP Aronstein, K (reprint author), USDA ARS, Honey Bee Res Unit, Weslaco, TX 78596 USA. EM Kate.Aronstein@ars.usda.gov RI Evans, Jay/C-8408-2012; Elsik, Christine/C-4120-2017; OI Evans, Jay/0000-0002-0036-4651; Elsik, Christine/0000-0002-4248-7713; Childers, Anna/0000-0002-0747-8539 FU Agricultural Research Service of the USDA, CRIS Project [6204-21000-009-00D]; USDA NIFA [2010-65106-20634]; Managed Pollinator CAP USDA NIFA [20098511805718] FX We thank Dr. Phillip San Miguel and Dr. Greg Hunt (Purdue University, Lafayette, IN) for assistance with transcriptome sequencing. This study was supported by the Agricultural Research Service of the USDA, CRIS Project Number 6204-21000-009-00D, USDA NIFA 2010-65106-20634 and Managed Pollinator CAP USDA NIFA 20098511805718. NR 65 TC 12 Z9 12 U1 3 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 29 PY 2012 VL 13 AR 285 DI 10.1186/1471-2164-13-285 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 994RH UT WOS:000307948500001 PM 22747707 ER PT J AU Carriere, Y Goodell, PB Ellers-Kirk, C Larocque, G Dutilleul, P Naranjo, SE Ellsworth, PC AF Carriere, Yves Goodell, Peter B. Ellers-Kirk, Christa Larocque, Guillaume Dutilleul, Pierre Naranjo, Steven E. Ellsworth, Peter C. TI Effects of Local and Landscape Factors on Population Dynamics of a Cotton Pest SO PLOS ONE LA English DT Article ID HEMIPTERA-MIRIDAE; HOST PLANTS; LYGUS; ALFALFA; MODELS; SUPPRESSION; RESISTANCE; PREDATORS; MOVEMENTS; SAFFLOWER AB Background: Many polyphagous pests sequentially use crops and uncultivated habitats in landscapes dominated by annual crops. As these habitats may contribute in increasing or decreasing pest density in fields of a specific crop, understanding the scale and temporal variability of source and sink effects is critical for managing landscapes to enhance pest control. Methodology/Principal Findings: We evaluated how local and landscape characteristics affect population density of the western tarnished plant bug, Lygus hesperus (Knight), in cotton fields of the San Joaquin Valley in California. During two periods covering the main window of cotton vulnerability to Lygus attack over three years, we examined the associations between abundance of six common Lygus crops, uncultivated habitats and Lygus population density in these cotton fields. We also investigated impacts of insecticide applications in cotton fields and cotton flowering date. Consistent associations observed across periods and years involved abundances of cotton and uncultivated habitats that were negatively associated with Lygus density, and abundance of seed alfalfa and cotton flowering date that were positively associated with Lygus density. Safflower and forage alfalfa had variable effects, possibly reflecting among-year variation in crop management practices, and tomato, sugar beet and insecticide applications were rarely associated with Lygus density. Using data from the first two years, a multiple regression model including the four consistent factors successfully predicted Lygus density across cotton fields in the last year of the study. Conclusions/Significance: Our results show that the approach developed here is appropriate to characterize and test the source and sink effects of various habitats on pest dynamics and improve the design of landscape-level pest management strategies. C1 [Carriere, Yves; Ellers-Kirk, Christa; Ellsworth, Peter C.] Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. [Goodell, Peter B.] Univ Calif Cooperat Extens, Kearney Agr Ctr, Parlier, CA USA. [Larocque, Guillaume; Dutilleul, Pierre] McGill Univ, Dept Plant Sci, Ste Anne De Bellevue, PQ, Canada. [Naranjo, Steven E.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ USA. RP Carriere, Y (reprint author), Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA. EM ycarrier@ag.arizona.edu OI Ellsworth, Peter/0000-0002-2485-0830; Larocque, Guillaume/0000-0002-5967-9156 FU USDA-CSREES RAMP [0207436] FX This work was funded by USDA-CSREES RAMP, Developing and Implementing Field and Landscape Level Reduced-Risk Management Strategies for Lygus in Western Cropping Systems (Project #0207436). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 16 Z9 16 U1 3 U2 46 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 29 PY 2012 VL 7 IS 6 AR e39862 DI 10.1371/journal.pone.0039862 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 967FO UT WOS:000305892100116 PM 22768147 ER PT J AU Liu, ZJ Kumari, S Zhang, LF Zheng, YL Ware, D AF Liu, Zhijie Kumari, Sunita Zhang, Lifang Zheng, Yonglian Ware, Doreen TI Characterization of miRNAs in Response to Short-Term Waterlogging in Three Inbred Lines of Zea mays SO PLOS ONE LA English DT Article ID STRESS-REGULATED MICRORNAS; BOX TRANSCRIPTION FACTORS; DNA-BINDING SPECIFICITY; BARLEY ALEURONE CELLS; PEA PLASTOCYANIN GENE; ZINC-FINGER PROTEIN; LOOP RT-PCR; ARABIDOPSIS-THALIANA; ABSCISIC-ACID; PLANT MICRORNAS AB Waterlogging of plants leads to low oxygen levels (hypoxia) in the roots and causes a metabolic switch from aerobic respiration to anaerobic fermentation that results in rapid changes in gene transcription and protein synthesis. Our research seeks to characterize the microRNA-mediated gene regulatory networks associated with short-term waterlogging. MicroRNAs (miRNAs) are small non-coding RNAs that regulate many genes involved in growth, development and various biotic and abiotic stress responses. To characterize the involvement of miRNAs and their targets in response to short-term hypoxia conditions, a quantitative real time PCR (qRT-PCR) assay was used to quantify the expression of the 24 candidate mature miRNA signatures (22 known and 2 novel mature miRNAs, representing 66 miRNA loci) and their 92 predicted targets in three inbred Zea mays lines (waterlogging tolerant Hz32, mid-tolerant B73, and sensitive Mo17). Based on our studies, miR159, miR164, miR167, miR393, miR408 and miR528, which are mainly involved in root development and stress responses, were found to be key regulators in the post-transcriptional regulatory mechanisms under short-term waterlogging conditions in three inbred lines. Further, computational approaches were used to predict the stress and development related cis-regulatory elements on the promoters of these miRNAs; and a probable miRNA-mediated gene regulatory network in response to short-term waterlogging stress was constructed. The differential expression patterns of miRNAs and their targets in these three inbred lines suggest that the miRNAs are active participants in the signal transduction at the early stage of hypoxia conditions via a gene regulatory network; and crosstalk occurs between different biochemical pathways. C1 [Liu, Zhijie; Zheng, Yonglian] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China. [Liu, Zhijie; Kumari, Sunita; Zhang, Lifang; Ware, Doreen] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA. [Ware, Doreen] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Liu, ZJ (reprint author), Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Hubei, Peoples R China. EM yonglianzheng@gmail.com; ware@cshl.edu FU USDA [1907-21000-030]; project 111, Ministry of Education of China and State Administration of Foreign Experts Affairs of China [B07041] FX This work was supported by a USDA grant (1907-21000-030, www.usda.gov), and project 111 (B07041, Ministry of Education of China and State Administration of Foreign Experts Affairs of China) (http://www.moe.gov.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 122 TC 33 Z9 33 U1 4 U2 36 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 29 PY 2012 VL 7 IS 6 AR e39786 DI 10.1371/journal.pone.0039786 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 967FO UT WOS:000305892100093 PM 22768123 ER PT J AU Long, DP Zhao, AC Chen, XJ Zhang, Y Lu, WJ Guo, Q Handler, AM Xiang, ZH AF Long, Ding-Pei Zhao, Ai-Chun Chen, Xue-Jiao Zhang, Yang Lu, Wei-Jian Guo, Qing Handler, Alfred M. Xiang, Zhong-Huai TI FLP Recombinase-Mediated Site-Specific Recombination in Silkworm, Bombyx mori SO PLOS ONE LA English DT Article ID GERMLINE TRANSFORMATION; TRANSGENIC SILKWORMS; PHI-C31 INTEGRASE; DROSOPHILA-MELANOGASTER; ENHANCER DETECTION; CASSETTE EXCHANGE; 2-MU CIRCLE; CELL-LINES; GENE; TRANSPOSON AB A comprehensive understanding of gene function and the production of site-specific genetically modified mutants are two major goals of genetic engineering in the post-genomic era. Although site-specific recombination systems have been powerful tools for genome manipulation of many organisms, they have not yet been established for use in the manipulation of the silkworm Bombyx mori genome. In this study, we achieved site-specific excision of a target gene at predefined chromosomal sites in the silkworm using a FLP/FRT site-specific recombination system. We first constructed two stable transgenic target silkworm strains that both contain a single copy of the transgene construct comprising a target gene expression cassette flanked by FRT sites. Using pre-blastoderm microinjection of a FLP recombinase helper expression vector, 32 G3 site-specific recombinant transgenic individuals were isolated from five of 143 broods. The average frequency of FLP recombinase-mediated site-specific excision in the two target strains genome was approximately 3.5%. This study shows that it is feasible to achieve site-specific recombination in silkworms using the FLP/FRT system. We conclude that the FLP/FRT system is a useful tool for genome manipulation in the silkworm. Furthermore, this is the first reported use of the FLP/FRT system for the genetic manipulation of a lepidopteran genome and thus provides a useful reference for the establishment of genome manipulation technologies in other lepidopteran species. C1 [Long, Ding-Pei; Zhao, Ai-Chun; Chen, Xue-Jiao; Zhang, Yang; Lu, Wei-Jian; Guo, Qing; Xiang, Zhong-Huai] Southwest Univ, Inst Sericulture & Syst Biol, State Key Lab Silkworm Genome Biol, Chongqing, Peoples R China. [Handler, Alfred M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Long, DP (reprint author), Southwest Univ, Inst Sericulture & Syst Biol, State Key Lab Silkworm Genome Biol, Chongqing, Peoples R China. EM zhaoaichun@hotmail.com FU Chongqing Science & Technology Commission [CSTC, 2011BA1005, CSTC, 2009AA1024]; Fundamental Research Funds for the Central Universities [XDJK2010B015] FX This work was supported by Chongqing Science & Technology Commission (No. CSTC, 2011BA1005 and CSTC, 2009AA1024) and the Fundamental Research Funds for the Central Universities (No. XDJK2010B015). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 21 Z9 22 U1 0 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 29 PY 2012 VL 7 IS 6 AR e40150 DI 10.1371/journal.pone.0040150 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 967FO UT WOS:000305892100218 PM 22768245 ER PT J AU Baxter, I Dilkes, BP AF Baxter, Ivan Dilkes, Brian P. TI Elemental Profiles Reflect Plant Adaptations to the Environment SO SCIENCE LA English DT Editorial Material ID ARABIDOPSIS-THALIANA; MINERAL CONCENTRATIONS; SERPENTINE SOILS; LEAF IONOME; IDENTIFICATION; TOLERANCE; EFFLUX; BORON; PANEL; QTL AB Most mineral elements found in plant tissues come exclusively from the soil, necessitating that plants adapt to highly variable soil compositions to survive and thrive. Profiling element concentrations in genetically diverse plant populations is providing insights into the plant-environment interactions that control elemental accumulation, as well as identifying the underlying genes. The resulting molecular understanding of plant adaptation to the environment both demonstrates how soils can shape genetic diversity and provides solutions to important agricultural challenges. C1 [Baxter, Ivan] USDA ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Dilkes, Brian P.] Purdue Univ, W Lafayette, IN 47907 USA. RP Baxter, I (reprint author), USDA ARS, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. EM ivan.baxter@ars.usda.gov; bdilkes@purdue.edu RI Baxter, Ivan/A-1052-2009; Dilkes, Brian/C-9293-2012 OI Baxter, Ivan/0000-0001-6680-1722; Dilkes, Brian/0000-0003-2799-954X NR 28 TC 22 Z9 24 U1 7 U2 75 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD JUN 29 PY 2012 VL 336 IS 6089 BP 1661 EP 1663 DI 10.1126/science.1219992 PG 3 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 965VD UT WOS:000305794500037 PM 22745418 ER PT J AU Powell, ALT Nguyen, CV Hill, T Cheng, KL Figueroa-Balderas, R Aktas, H Ashrafi, H Pons, C Fernandez-Munoz, R Vicente, A Lopez-Baltazar, J Barry, CS Liu, YS Chetelat, R Granell, A Van Deynze, A Giovannoni, JJ Bennett, AB AF Powell, Ann L. T. Nguyen, Cuong V. Hill, Theresa Cheng, Kalai Lam Figueroa-Balderas, Rosa Aktas, Hakan Ashrafi, Hamid Pons, Clara Fernandez-Munoz, Rafael Vicente, Ariel Lopez-Baltazar, Javier Barry, Cornelius S. Liu, Yongsheng Chetelat, Roger Granell, Antonio Van Deynze, Allen Giovannoni, James J. Bennett, Alan B. TI Uniform ripening Encodes a Golden 2-like Transcription Factor Regulating Tomato Fruit Chloroplast Development SO SCIENCE LA English DT Article ID SOLUBLE SOLIDS CONTENT; GENE FAMILY; EXPRESSION; PHOTOSYNTHESIS; SPECIALIZATION; NUCLEAR AB Modern tomato (Solanum lycopersicum) varieties are bred for uniform ripening (u) light green fruit phenotypes to facilitate harvests of evenly ripened fruit. U encodes a Golden 2-like (GLK) transcription factor, SlGLK2, which determines chlorophyll accumulation and distribution in developing fruit. In tomato, two GLKs-SlGLK1 and SlGLK2-are expressed in leaves, but only SlGLK2 is expressed in fruit. Expressing GLKs increased the chlorophyll content of fruit, whereas SlGLK2 suppression recapitulated the u mutant phenotype. GLK overexpression enhanced fruit photosynthesis gene expression and chloroplast development, leading to elevated carbohydrates and carotenoids in ripe fruit. SlGLK2 influences photosynthesis in developing fruit, contributing to mature fruit characteristics and suggesting that selection of u inadvertently compromised ripe fruit quality in exchange for desirable production traits. C1 [Powell, Ann L. T.; Hill, Theresa; Cheng, Kalai Lam; Figueroa-Balderas, Rosa; Aktas, Hakan; Ashrafi, Hamid; Vicente, Ariel; Lopez-Baltazar, Javier; Chetelat, Roger; Van Deynze, Allen; Bennett, Alan B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Nguyen, Cuong V.; Giovannoni, James J.] Cornell Univ, Dept Plant Breeding, Ithaca, NY 14853 USA. [Nguyen, Cuong V.; Giovannoni, James J.] Cornell Univ, Dept Genet & Plant Biol, Ithaca, NY 14853 USA. [Pons, Clara; Granell, Antonio] Univ Politecn Valencia, Inst Biol Mol & Celular Plantas, Consejo Super Invest Cient, E-46022 Valencia, Spain. [Fernandez-Munoz, Rafael] Univ Malaga, Inst Hort Subtrop & Mediterranea Mayora, Consejo Super Invest Cient, E-29750 Malaga, Spain. [Vicente, Ariel] UNLP, Ctr Invest & Desarrollo Criotecnol Alimentos, Consejo Nacl Invest Cient & Tecn, RA-1900 La Plata, Argentina. [Vicente, Ariel] Univ Nacl La Plata, Fac Ciencias Agr & Forestales, RA-1900 La Plata, Argentina. [Barry, Cornelius S.] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Liu, Yongsheng; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Liu, Yongsheng; Giovannoni, James J.] Cornell Univ, USDA ARS, Robert W Holley Ctr, Ithaca, NY 14853 USA. RP Powell, ALT (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM alpowell@ucdavis.edu; jjg33@cornell.edu RI Barry, Cornelius/D-7759-2012; Bennett, Alan/D-8980-2011; Figueroa, Rosa/B-3172-2013; AKTAS, Hakan/E-9174-2013; Granell, Antonio/G-3664-2010; Fernandez-Munoz, Rafael/F-3714-2010; OI Bennett, Alan/0000-0002-3684-4307; Fernandez-Munoz, Rafael/0000-0002-4054-8949; Figueroa-Balderas, Rosa/0000-0003-3321-1376; Barry, Cornelius/0000-0003-4685-0273; Vicente, Ariel/0000-0003-1289-9554; Pons, Clara/0000-0002-8296-2937 FU University of California; Vietnam Education Foundation; USDA-Agricultural Research Service; USDA-National Research Initiative [2007-02773]; NSF [IOS-0923312] FX Minimum Information About a Microarray Experiment (MIAME)-compliant microarray data are available at http://ted.bti.cornell.edu. and at http://www.ebi.ac.uk/arrayexpress/(accession E-MEXP-3652). F. Carrari and A. Fernie provided S. pennellii SlGLK2, and J. Maloof provided S. habrochaites SlGLK2 sequences. The U. S. Department of Agriculture (USDA)/National Institute of Food and Agriculture Solanaceae Coordinated Agricultural Project provided potato data. We are grateful to the Tomato Genome Consortium and the SOL Genomics Network for prepublication access to the tomato genome sequence. The S. pennellii introgression lines were provided by the C. M. Rick Tomato Genetics Resource Center; the S. pimpinellifolium populations were provided by the Instituto de Hortofruticultura Subtropical y Mediterranea "La Mayora," Consejo Superior de Investigaciones Cientificas; and both populations are available by request from the sources. The AtGLK-expressing lines were provided by Mendel Biotechnology and Seminis/Monsanto Vegetable Seeds. SlGLK2, the corresponding lines, and the F2 10-1 IL x M82 population lines and seeds are available from J. J. G. without restriction. Seminis/Monsanto will make available, upon request, and under a material transfer agreement indicating they are to be used for noncommercial purposes, the following lines: LexA:AtGLK1:p35S:LexA-Gal4; LexA:AtGLK1:pLTP:LexA-Gal4; LexA:AtGLK1:pRbcS:LexA-Gal4; LexA:AtGLK1:pPDS:LexA-Gal4; LexA:AtGLK2:p35S:LexA-Gal4; LexA:AtGLK2:pLTP:LexA-Gal4; LexA:AtGLK2:pRbcS:LexA-Gal4; LexA:AtGLK2:pPDS:LexA-Gal4; plus the T63 control line. Other biological materials are available by request from A. L. T. P. or J.J.G. A. L. T. P., T. H., K.L.-C., R.F.-B., and A. B. B. have filed a provisional U. S. patent application UC #2011-841, "Introduction of wild species GLK genes for improved ripe tomato fruit quality," through the University of California. A. L. T. P. and A. B. B. have filed the U. S. patent application #2010/0154078, " Transcription factors that enhance traits in plant organs," through Mendel Biotechnology. Assistance from B. Blanco-Ulate, S. Phothiset, S. Reyes, A. Abraham, L. Gilani, and G. Arellano is gratefully acknowledged. J. Langdale provided helpful advice regarding GLK phylogeny and nomenclature. G. Adamson and P. Kysar, Electron Microscopy (EM) Laboratory, University of California Davis Medical Center did the EM work. University of California Discovery and partners funded the pepper analysis and the initial investigations of the Arabidopsis GLKs. The Vietnam Education Foundation supported C.N. Fundacion Genoma Espana ESPSOL Project provided partial funding to A.G. USDA-Agricultural Research Service, USDA-National Research Initiative (2007-02773), and NSF (Plant Genome Program IOS-0923312) provided support to J.J.G. NR 28 TC 90 Z9 96 U1 10 U2 164 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JUN 29 PY 2012 VL 336 IS 6089 BP 1711 EP 1715 DI 10.1126/science.1222218 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 965VD UT WOS:000305794500052 PM 22745430 ER PT J AU Floudas, D Binder, M Riley, R Barry, K Blanchette, RA Henrissat, B Martinez, AT Otillar, R Spatafora, JW Yadav, JS Aerts, A Benoit, I Boyd, A Carlson, A Copeland, A Coutinho, PM de Vries, RP Ferreira, P Findley, K Foster, B Gaskell, J Glotzer, D Gorecki, P Heitman, J Hesse, C Hori, C Igarashi, K Jurgens, JA Kallen, N Kersten, P Kohler, A Kues, U Kumar, TKA Kuo, A LaButti, K Larrondo, LF Lindquist, E Ling, A Lombard, V Lucas, S Lundell, T Martin, R McLaughlin, DJ Morgenstern, I Morin, E Murat, C Nagy, LG Nolan, M Ohm, RA Patyshakuliyeva, A Rokas, A Ruiz-Duenas, FJ Sabat, G Salamov, A Samejima, M Schmutz, J Slot, JC John, FS Stenlid, J Sun, H Sun, S Syed, K Tsang, A Wiebenga, A Young, D Pisabarro, A Eastwood, DC Martin, F Cullen, D Grigoriev, IV Hibbett, DS AF Floudas, Dimitrios Binder, Manfred Riley, Robert Barry, Kerrie Blanchette, Robert A. Henrissat, Bernard Martinez, Angel T. Otillar, Robert Spatafora, Joseph W. Yadav, Jagjit S. Aerts, Andrea Benoit, Isabelle Boyd, Alex Carlson, Alexis Copeland, Alex Coutinho, Pedro M. de Vries, Ronald P. Ferreira, Patricia Findley, Keisha Foster, Brian Gaskell, Jill Glotzer, Dylan Gorecki, Pawel Heitman, Joseph Hesse, Cedar Hori, Chiaki Igarashi, Kiyohiko Jurgens, Joel A. Kallen, Nathan Kersten, Phil Kohler, Annegret Kuees, Ursula Kumar, T. K. Arun Kuo, Alan LaButti, Kurt Larrondo, Luis F. Lindquist, Erika Ling, Albee Lombard, Vincent Lucas, Susan Lundell, Taina Martin, Rachael McLaughlin, David J. Morgenstern, Ingo Morin, Emanuelle Murat, Claude Nagy, Laszlo G. Nolan, Matt Ohm, Robin A. Patyshakuliyeva, Aleksandrina Rokas, Antonis Ruiz-Duenas, Francisco J. Sabat, Grzegorz Salamov, Asaf Samejima, Masahiro Schmutz, Jeremy Slot, Jason C. John, Franz St. Stenlid, Jan Sun, Hui Sun, Sheng Syed, Khajamohiddin Tsang, Adrian Wiebenga, Ad Young, Darcy Pisabarro, Antonio Eastwood, Daniel C. Martin, Francis Cullen, Dan Grigoriev, Igor V. Hibbett, David S. TI The Paleozoic Origin of Enzymatic Lignin Decomposition Reconstructed from 31 Fungal Genomes SO SCIENCE LA English DT Article ID PHANEROCHAETE-CHRYSOSPORIUM; WOOD DECAY; EVOLUTION; GENE; MODELS; DEGRADATION; PEROXIDASES; CELLULOSE; SEQUENCE; FAMILY AB Wood is a major pool of organic carbon that is highly resistant to decay, owing largely to the presence of lignin. The only organisms capable of substantial lignin decay are white rot fungi in the Agaricomycetes, which also contains non-lignin-degrading brown rot and ectomycorrhizal species. Comparative analyses of 31 fungal genomes (12 generated for this study) suggest that lignin-degrading peroxidases expanded in the lineage leading to the ancestor of the Agaricomycetes, which is reconstructed as a white rot species, and then contracted in parallel lineages leading to brown rot and mycorrhizal species. Molecular clock analyses suggest that the origin of lignin degradation might have coincided with the sharp decrease in the rate of organic carbon burial around the end of the Carboniferous period. C1 [Riley, Robert; Barry, Kerrie; Otillar, Robert; Aerts, Andrea; Copeland, Alex; Foster, Brian; Kuo, Alan; LaButti, Kurt; Lindquist, Erika; Lucas, Susan; Nolan, Matt; Ohm, Robin A.; Salamov, Asaf; Sun, Hui; Grigoriev, Igor V.] US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. [Floudas, Dimitrios; Binder, Manfred; Carlson, Alexis; Glotzer, Dylan; Kallen, Nathan; Ling, Albee; Martin, Rachael; Nagy, Laszlo G.; Young, Darcy; Hibbett, David S.] Clark Univ, Dept Biol, Worcester, MA 01610 USA. [Blanchette, Robert A.; Jurgens, Joel A.; Kumar, T. K. Arun; McLaughlin, David J.] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA. [Henrissat, Bernard; Coutinho, Pedro M.; Lombard, Vincent] Aix Marseille Univ, CNRS, UMR 6098, F-13288 Marseille 9, France. [Martinez, Angel T.; Ruiz-Duenas, Francisco J.] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain. [Spatafora, Joseph W.; Boyd, Alex; Hesse, Cedar] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Yadav, Jagjit S.; Syed, Khajamohiddin] Univ Cincinnati, Coll Med, Dept Environm Hlth, Environm Genet & Mol Toxicol Div, Cincinnati, OH 45267 USA. [Benoit, Isabelle; de Vries, Ronald P.] Univ Utrecht, Microbiol & Kluyver Ctr Genom Ind Fermentat, NL-3584 CH Utrecht, Netherlands. [Benoit, Isabelle; de Vries, Ronald P.; Patyshakuliyeva, Aleksandrina; Wiebenga, Ad] CBS KNAW Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands. [Ferreira, Patricia] Univ Zaragoza, Dept Biochem & Mol & Cellular Biol, E-50009 Zaragoza, Spain. [Ferreira, Patricia] Univ Zaragoza, Inst Biocomputat & Phys Complex Syst, E-50009 Zaragoza, Spain. [Findley, Keisha; Heitman, Joseph; Sun, Sheng] Duke Univ, Med Ctr, Dept Mol Genet & Microbiol, Durham, NC 27710 USA. [Gaskell, Jill; Kersten, Phil; John, Franz St.; Cullen, Dan] USDA, Forest Prod Lab, Madison, WI 53726 USA. [Gorecki, Pawel] Warsaw Univ, Inst Informat, PL-02097 Warsaw, Poland. [Hori, Chiaki; Igarashi, Kiyohiko; Samejima, Masahiro] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomat Sci, Bunkyo Ku, Tokyo 1138657, Japan. [Kohler, Annegret; Morin, Emanuelle; Murat, Claude; Martin, Francis] Nancy Univ, INRA, UMR 1136, F-54280 Champenoux, France. [Kuees, Ursula] Univ Gottingen, Busgen Inst, D-37077 Gottingen, Germany. [Larrondo, Luis F.] Pontificia Univ Catolica Chile, Fac Ciencias Biol, Dept Mol Genet & Microbiol, Santiago 8331010, Chile. [Lundell, Taina] Univ Helsinki, Viikki Bioctr, Dept Appl Chem & Microbiol, FIN-00014 Helsinki, Finland. [Morgenstern, Ingo; Tsang, Adrian] Concordia Univ, Ctr Struct & Funct Genom, Montreal, PQ H4B 1R6, Canada. [Rokas, Antonis; Slot, Jason C.] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37235 USA. [Sabat, Grzegorz] Univ Wisconsin, Ctr Biotechnol, Madison, WI 53726 USA. [Schmutz, Jeremy] HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA. [Stenlid, Jan] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, S-75007 Uppsala, Sweden. [Pisabarro, Antonio] Univ Publ Navarra, Genet & Microbiol Res Grp, Pamplona 31006, Spain. [Eastwood, Daniel C.] Univ Swansea, Coll Sci, Swansea SA2 8PP, W Glam, Wales. RP Grigoriev, IV (reprint author), US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA. EM ivgrigoriev@lbl.gov; dhibbett@clarku.edu RI Henrissat, Bernard/J-2475-2012; de Vries, Ronald/F-8125-2011; Larrondo, Luis/A-2916-2013; Pisabarro, Antonio/K-3622-2014; Ruiz-Duenas, Francisco/L-9837-2015; Rokas, Antonis/A-9775-2008; Igarashi, Kiyohiko/E-6799-2016; Binder, Manfred/C-8571-2013; Gorecki, Pawel/D-3160-2013; Slot, Jason/E-4802-2011; Syed, Khajamohiddin/L-8774-2013; Schmutz, Jeremy/N-3173-2013; Ohm, Robin/I-6689-2016; Larrondo, Luis/J-1086-2016; St John, Franz/J-8970-2016; Lundell, Taina/P-6623-2016; OI de Vries, Ronald/0000-0002-4363-1123; Pisabarro, Antonio/0000-0001-6987-5794; Ruiz-Duenas, Francisco/0000-0002-9837-5665; Rokas, Antonis/0000-0002-7248-6551; Igarashi, Kiyohiko/0000-0001-5152-7177; Gorecki, Pawel/0000-0002-2045-5892; Syed, Khajamohiddin/0000-0002-1497-3570; Schmutz, Jeremy/0000-0001-8062-9172; Larrondo, Luis/0000-0002-8832-7109; St John, Franz/0000-0003-3458-5628; Lundell, Taina/0000-0003-3899-1658; Eastwood, Daniel/0000-0002-7015-0739; Martinez, Angel T/0000-0002-1584-2863; Kues, Ursula/0000-0001-9180-4079 FU Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]; Assembling the Fungal Tree of Life (AFTOL) project under NSF [DEB-0732968, DEB-0732993, DEB-0732550] FX The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract DE-AC02-05CH11231. Also supported by the Assembling the Fungal Tree of Life (AFTOL) project under NSF awards DEB-0732968 (D. S. H.), DEB-0732993 (J.W.S.), and DEB-0732550 (D.J.M.). We thank R. H. Petersen for the strain of A. delicata. The organisms A. delicata and Dacryopinax sp. were obtained in Costa Rica and can only be used for research purposes. Assemblies and annotations of the 12 genomes reported here are available from the JGI fungal portal MycoCosm (http://jgi.doe.gov/fungi) and from DDBJ/EMBL/GenBank under the following accessions: AFVO00000000, AEIT00000000, AEUS00000000, AEID00000000, AEJJ00000000, AEHC00000000, AFVP00000000, AEGM00000000, AEGX00000000, AEJI00000000, AFVY00000000, and AEHD00000000. Aligned sequence data for organismal and gene family phylogenies and molecular clock analyses, and secretome results are available at DRYAD (http://dx.doi.org/10.5061/dryad.5k3t47p0). NR 25 TC 392 Z9 404 U1 45 U2 425 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 EI 1095-9203 J9 SCIENCE JI Science PD JUN 29 PY 2012 VL 336 IS 6089 BP 1715 EP 1719 DI 10.1126/science.1221748 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 965VD UT WOS:000305794500053 PM 22745431 ER PT J AU Ching, KH Lin, A McGarvey, JA Stanker, LH Hnasko, R AF Ching, Kathryn H. Lin, Alice McGarvey, Jeffery A. Stanker, Larry H. Hnasko, Robert TI Rapid and selective detection of botulinum neurotoxin serotype-A and -B with a single immunochromatographic test strip SO JOURNAL OF IMMUNOLOGICAL METHODS LA English DT Article DE Lateral flow device; Immunoassay; Botulinum; Toxin; Serotype; Diagnostic ID CLOSTRIDIUM-BOTULINUM; MOUSE BIOASSAY; MONOCLONAL-ANTIBODIES; SANDWICH ELISA; TOXIN; ASSAY; FOODS; DIAGNOSIS; WEAPON AB Botulinum neurotoxins (BoNT) are the most potent toxins known. Produced by Clostridium botulinum, BoNTs are classified into seven, antigenically distinct serotypes, designated A-G. The toxin acts to inhibit acetylcholine release, resulting in paralysis and death. Naturally occurring foodborne disease is most often the result of improper canning of foods, while wound botulism, associated with injection drug users, is on the rise. Because of its potency, BoNTs have also been identified as targets for use by bioterrorists. The 'gold standard' of detection of BoNTs is the mouse bioassay, an expensive and time consuming test that requires specialized equipment and trained personnel. There is a need for a rapid, sensitive diagnostic for BoNTs that could be used by minimally trained personnel in the event of a foodborne outbreak or a bioterrorist threat. Here, we describe the use of a single lateral flow device (LFD) that can detect and distinguish between BoNT/A and B, two of the four serotypes that are known to intoxicate humans and together represent >80% of naturally occurring illness. The device could detect as little as 5 ng/mL of purified BoNT/A and 10 ng/mL of BoNT/B in 2% and 1% milk, respectively. In undiluted apple juice, 25 ng/mL of BoNT/A and 10 ng/mL of BoNT/B could be detected. No cross reactivity between BoNT/A and B antibodies was observed. The LFD described here is easy to use, requires no specialized training or equipment, and can identify and distinguish between BoNT/A and /B serotypes. These attributes make this rapid diagnostic device a potentially valuable tool in the fields of food safety and homeland security. Published by Elsevier B.V. C1 [Ching, Kathryn H.; Lin, Alice; McGarvey, Jeffery A.; Stanker, Larry H.; Hnasko, Robert] USDA, ARS, WRRC, Albany, CA 94710 USA. RP Hnasko, R (reprint author), USDA, ARS, WRRC, 800 Buchanan St,Rm 2124, Albany, CA 94710 USA. EM Robert.Hnasko@ars.usda.gov NR 26 TC 30 Z9 32 U1 0 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1759 J9 J IMMUNOL METHODS JI J. Immunol. Methods PD JUN 29 PY 2012 VL 380 IS 1-2 BP 23 EP 29 DI 10.1016/j.jim.2012.03.008 PG 7 WC Biochemical Research Methods; Immunology SC Biochemistry & Molecular Biology; Immunology GA 960BF UT WOS:000305362300004 PM 22504369 ER PT J AU Stanchi, S Freppaz, M Agnelli, A Reinsch, T Zanini, E AF Stanchi, S. Freppaz, M. Agnelli, A. Reinsch, T. Zanini, E. TI Properties, best management practices and conservation of terraced soils in Southern Europe (from Mediterranean areas to the Alps): A review SO QUATERNARY INTERNATIONAL LA English DT Review ID CENTRAL SPANISH PYRENEES; LAND-USE CHANGES; REGION NE SPAIN; AGRICULTURAL TERRACES; MOUNTAIN ENVIRONMENTS; FARMLAND ABANDONMENT; SEMIARID ENVIRONMENT; WATER CONSERVATION; BENCH TERRACES; PERUVIAN ANDES AB Terrace soils are distinctive features of the agricultural landscape in Europe. Due to their historical and aesthetic significance, they are a resource for agriculture and tourism: however they are also a challenge for land conservation and management. Nevertheless, the fundamental role of terrace soils for agricultural quality and natural hazard prevention has not been fully investigated. In the past, terraced slopes became ideal sites for human settlement and agricultural activities. At present, they are often used for high quality crops that grow on soils with specific chemical and physical properties. The filling material used for building terraces is a human-reworked substrate, where pedogenesis occurs under a strong human influence. This leads to soils with a generally limited pedogenic development and coarse texture, although, often, with good productivity. When abandoned, terraces are subjected to progressive decay due to erosion processes and slope failures. This review focuses on terrace soil properties, conservation and management in Southern Europe. In particular, it reports some examples from the northwestern Italian Alps, where terraced slopes are characterised by ancient origin and, presently, are subjected to specific practices for their preservation. The different effects of land degradation at hill slope scale may be mitigated through appropriate management practices favoring the terrace walls maintenance, drainage optimization, and the presence of a spontaneous vegetation cover. These subjects require careful planning and conservation measures that could be collected in "best practices" guidelines for farmers, landowners and decision-makers. (C) 2011 Elsevier Ltd and INQUA. All rights reserved. C1 [Stanchi, S.; Freppaz, M.; Zanini, E.] Univ Torino, DIVAPRA Agr Chem & Pedol, I-10095 Grugliasco, TO, Italy. [Agnelli, A.] Univ Perugia, Dipartimento Sci Agr & Ambientali, I-06121 Perugia, Italy. [Reinsch, T.] USDA NRCS, Natl Soil Survey Ctr, Lincoln, NE 68508 USA. [Stanchi, S.; Freppaz, M.; Zanini, E.] NATRISK Res Ctr Nat Risks Mt & Hilly Enviroments, I-10095 Grugliasco, TO, Italy. RP Stanchi, S (reprint author), Univ Torino, DIVAPRA Agr Chem & Pedol, Via Leonardo da Vinci 44, I-10095 Grugliasco, TO, Italy. EM silvia.stanchi@unito.it OI Stanchi, Silvia/0000-0002-2167-4421; Agnelli, Alberto/0000-0002-2236-9103 FU EU FX This work was funded by EU project Alpter (www.alpter.net). Special thanks to Dr Cristina Galliani and Dr Valeria Revel Chion (Regione Valle d'Aosta Administration, Assessorato Agricoltura e Risorse Naturali) technical support, and to the Project Partners for cooperation throughout the work. NR 81 TC 26 Z9 26 U1 9 U2 62 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1040-6182 EI 1873-4553 J9 QUATERN INT JI Quat. Int. PD JUN 28 PY 2012 VL 265 BP 90 EP 100 DI 10.1016/j.quaint.2011.09.015 PG 11 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA 972BH UT WOS:000306251000009 ER PT J AU Ennen, JR Lovich, JE Meyer, KP Bjurlin, C Arundel, TR AF Ennen, Joshua R. Lovich, Jeffrey E. Meyer, Katherin P. Bjurlin, Curtis Arundel, Terence R. TI Nesting Ecology of a Population of Gopherus agassizii at a Utility-Scale Wind Energy Facility in Southern California SO COPEIA LA English DT Article ID DESERT TORTOISE; EGG-PRODUCTION; MOJAVE DESERT; TURTLES; SURVIVAL; CONSERVATION; SIZE; DATE AB We investigated the annual nesting ecology of a population of Desert Tortoises (Gopherus agassizii) inhabiting a utility-scale renewable energy (USRE) facility in southern California and compared our results with populations inhabiting relatively undisturbed sites. In 2000, 15 radio-tracked females produced 29 clutches, and 24 nests were monitored to examine nest-site selection, nest predation, hatching success, date of emergence of hatchlings, and hatchling mass and carapace length. Overall, the nesting ecology of the population inhabiting the USRE facility was very similar to other populations of Desert Tortoises inhabiting relatively undisturbed habitats. Oviposition occurred from 12 May to 8 July, which was similar to other sites. Nest depths (11.1 cm), nest predation (12%), hatchling emergence date (7 August and 29 September), and hatchling morphometrics (i.e., MCL: 44.5 mm; mass: 23 g) were all within ranges reported in other populations. Unlike within other populations, we observed no relationship between hatchling size and either maternal body size or egg width. We found no evidence of females selecting for a particular burrow for oviposition of eggs based on environmental or anthropogenic variables. Most nests were located in or near burrows, and nest depth was greater for nests near the entrance than those deeper in the burrow. Although this study suggests that the nesting ecology of the Desert Tortoise population we studied was not adversely affected by the USRE facility, this relationship is only correlative because our study was not a before-after-control-impact (BACI) study, which would establish a cause and effect relationship. As pointed out in a recent review, BACI studies are critically needed to address the wildlife impacts of utility-scale renewable energy development. C1 [Ennen, Joshua R.; Lovich, Jeffrey E.; Arundel, Terence R.] US Geol Survey, SW Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Meyer, Katherin P.] US Forest Serv, Lytle Creek, CA 92358 USA. [Bjurlin, Curtis] Wind Capital Grp, Madison, WI 53705 USA. RP Ennen, JR (reprint author), Maryville Coll, Dept Biol, 502 E Lamar Alexander Pkwy, Maryville, TN 37804 USA. EM josh.ennen@maryvillecollege.edu; jeffrey_lovich@usgs.gov; kpmeyer@fs.fed.us; curtbjurlin@gmail.com; terry_arundel@usgs.gov OI Lovich, Jeffrey/0000-0002-7789-2831 FU California Energy Commission (Research Development and Demonstration Division, Public Interest Energy Research program) [500-09-020]; California Desert District Office of the Bureau of Land Management (BLM); Palm Springs-South Coast Field Office of BLM; Joshua Tree National Park FX Many people assisted us during the course of this study. Field support for this project was provided by interns from the Student Conservation Association and volunteers too numerous to list. Special thanks to R. Daniels, R. Griese, M. Massar, and G. Wright for their long commitment to the support of our project. Dr. M. Westbrooks allowed us to use his veterinary facilities to process X-radiographs. Research was supported by the California Energy Commission (Research Development and Demonstration Division, Public Interest Energy Research program [contract #500-09-020]), California Desert District Office of the Bureau of Land Management (BLM), the Palm Springs-South Coast Field Office of BLM, and Joshua Tree National Park. M. Agha assisted with temperature data collection. We thank K. Nussear of the U.S. Geological Survey for his helpful comments on an earlier version of this manuscript. Research was conducted under permits from the U.S. Fish and Wildlife Service, California Department of Fish and Game, and the BLM. Special thanks to A. Muth for providing accommodations at the Philip L. Boyd Deep Canyon Research Center of the University of California, Riverside, during the development of the manuscript. Use of product trade names does not constitute U.S. Geological Survey endorsement of any product. NR 34 TC 12 Z9 14 U1 4 U2 42 PU AMER SOC ICHTHYOLOGISTS & HERPETOLOGISTS PI MIAMI PA MAUREEN DONNELLY, SECRETARY FLORIDA INT UNIV BIOLOGICAL SCIENCES, 11200 SW 8TH STREET, MIAMI, FL 33199 USA SN 0045-8511 J9 COPEIA JI Copeia PD JUN 27 PY 2012 IS 2 BP 222 EP 228 DI 10.1643/CE-11-102 PG 7 WC Zoology SC Zoology GA 967SS UT WOS:000305928700006 ER PT J AU Boue, SM Isakova, IA Burow, ME Cao, HP Bhatnagar, D Sarver, JG Shinde, KV Erhardt, PW Heiman, ML AF Boue, Stephen M. Isakova, Iryna A. Burow, Matthew E. Cao, Heping Bhatnagar, Deepak Sarver, Jeff G. Shinde, Kamlesh V. Erhardt, Paul W. Heiman, Mark L. TI Glyceollins, Soy Isoflavone Phytoalexins, Improve Oral Glucose Disposal by Stimulating Glucose Uptake SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE glyceollins; isoflavones; diabetes; glucose; adipocytes; glucose transporters ID MOUSE 3T3-L1 ADIPOCYTES; GENE-EXPRESSION; HOMEOSTASIS; WOMEN; RISK; MICE AB Soy glyceollins, induced during stress, have been shown to inhibit cancer cell growth in vitro and in vivo. In the present study, we used prediabetic rats to examine the glyceollins effect on blood glucose. During an oral glucose tolerance test (OGTT), the blood glucose excursion was significantly decreased in the rats treated with oral administration of either 30 or 90 mg/kg glyceollins. Plasma analysis demonstrated that glyceollins are absorbed after oral administration, and duration of exposure extends from 20 min to at least 4 h postadministration. Exposure of 3T3-L1 adipocytes to glyceollins significantly increased both insulin-stimulated and basal glucose uptake. Basal glucose uptake was increased 1.5-fold by exposure to 5 mu M glyceollin in a dose-response manner. Coincubation with insulin significantly stimulated maximal glucose uptake above basal uptake levels and tended to increase glucose uptake beyond the levels of either stimulus alone. On a molecular level, polymerase chain reaction showed significantly increased levels of glucose transporter GLUT4 mRNA in 3T3-L1 adipocytes, especially when the cells were exposed to 5 mu M glyceollins for 3 h in vitro. It correlated with elevated protein levels of GLUT4 detected in the 5 mu M glyceollin-treated cells. Thus, the simulative effect of the glyceollins on adipocyte glucose uptake was attributed to up-regulation of glucose transporters. These findings indicate potential benefits of the glyceollins as an intervention in prediabetic conditions as well as a treatment for type 1 and type 2 diabetes by increasing both the insulin-mediated and the basal, insulin-independent, glucose uptake by adipocytes. C1 [Boue, Stephen M.; Cao, Heping; Bhatnagar, Deepak] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Isakova, Iryna A.; Burow, Matthew E.] Tulane Ctr Bioenvironm Res, New Orleans, LA 70118 USA. [Sarver, Jeff G.; Shinde, Kamlesh V.; Erhardt, Paul W.] Univ Toledo, Ctr Drug Design & Dev, Toledo, OH 43606 USA. [Heiman, Mark L.] NuMe Hlth, New Orleans, LA 70112 USA. RP Boue, SM (reprint author), USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM steve.boue@ars.usda.gov RI Burow, Matthew/D-6351-2013 OI Burow, Matthew/0000-0002-0642-6630 NR 28 TC 16 Z9 16 U1 3 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 27 PY 2012 VL 60 IS 25 BP 6376 EP 6382 DI 10.1021/jf301057d PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 964SK UT WOS:000305716800016 PM 22655912 ER PT J AU Ebbeling, CB Swain, JF Feldman, HA Wong, WW Hachey, DL Garcia-Lago, E Ludwig, DS AF Ebbeling, Cara B. Swain, Janis F. Feldman, Henry A. Wong, William W. Hachey, David L. Garcia-Lago, Erica Ludwig, David S. TI Effects of Dietary Composition on Energy Expenditure During Weight-Loss Maintenance SO JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION LA English DT Article ID MUSCLE WORK EFFICIENCY; LOW-GLYCEMIC LOAD; LOW-FAT DIET; SKELETAL-MUSCLE; INSULIN SENSITIVITY; RANDOMIZED-TRIAL; LOW-CARBOHYDRATE; OBESE ADULTS; RISK-FACTORS; BODY-WEIGHT AB Context Reduced energy expenditure following weight loss is thought to contribute to weight gain. However, the effect of dietary composition on energy expenditure during weight-loss maintenance has not been studied. Objective To examine the effects of 3 diets differing widely in macronutrient composition and glycemic load on energy expenditure following weight loss. Design, Setting, and Participants A controlled 3-way crossover design involving 21 overweight and obese young adults conducted at Children's Hospital Boston and Brigham and Women's Hospital, Boston, Massachusetts, between June 16, 2006, and June 21, 2010, with recruitment by newspaper advertisements and postings. Intervention After achieving 10% to 15% weight loss while consuming a run-in diet, participants consumed an isocaloric low-fat diet (60% of energy from carbohydrate, 20% from fat, 20% from protein; high glycemic load), low-glycemic index diet (40% from carbohydrate, 40% from fat, and 20% from protein; moderate glycemic load), and very low-carbohydrate diet (10% from carbohydrate, 60% from fat, and 30% from protein; low glycemic load) in random order, each for 4 weeks. Main Outcome Measures Primary outcome was resting energy expenditure (REE), with secondary outcomes of total energy expenditure (TEE), hormone levels, and metabolic syndrome components. Results Compared with the pre-weight-loss baseline, the decrease in REE was greatest with the low-fat diet (mean [95% CI], -205 [-265 to -144] kcal/d), intermediate with the low-glycemic index diet (-166 [-227 to -106] kcal/d), and least with the very low-carbohydrate diet (-138 [-198 to -77] kcal/d; overall P = .03; P for trend by glycemic load = .009). The decrease in TEE showed a similar pattern (mean [95% CI], -423 [-606 to -239] kcal/d; -297 [-479 to -115] kcal/d; and -97 [-281 to 86] kcal/d, respectively; overall P = .003; P for trend by glycemic load < .001). Hormone levels and metabolic syndrome components also varied during weight maintenance by diet (leptin, P < .001; 24-hour urinary cortisol, P = .005; indexes of peripheral [P = .02] and hepatic [P = .03] insulin sensitivity; high-density lipoprotein [HDL] cholesterol, P < .001; non-HDL cholesterol, P < .001; triglycerides, P < .001; plasminogen activator inhibitor 1, P for trend = .04; and C-reactive protein, P for trend = .05), but no consistent favorable pattern emerged. Conclusion Among overweight and obese young adults compared with pre-weight-loss energy expenditure, isocaloric feeding following 10% to 15% weight loss resulted in decreases in REE and TEE that were greatest with the low-fat diet, intermediate with the low-glycemic index diet, and least with the very low-carbohydrate diet. C1 [Ebbeling, Cara B.; Garcia-Lago, Erica; Ludwig, David S.] Childrens Hosp Boston, New Balance Fdn, Obes Prevent Ctr, Boston, MA 02115 USA. [Feldman, Henry A.] Childrens Hosp Boston, Clin Res Ctr, Boston, MA 02115 USA. [Swain, Janis F.] Brigham & Womens Hosp, Boston, MA 02115 USA. [Wong, William W.] ARS, Baylor Coll Med, USDA, Childrens Nutr Res Ctr, Houston, TX USA. [Hachey, David L.] Vanderbilt Univ, Nashville, TN USA. RP Ludwig, DS (reprint author), Childrens Hosp Boston, New Balance Fdn, Obes Prevent Ctr, 300 Longwood Ave, Boston, MA 02115 USA. EM david.ludwig@childrens.harvard.edu FU National Institutes of Health; foundations for obesity-related research; National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland [R01DK072428, K24DK082730]; National Center for Research Resources, National Institutes of Health [M01RR02172, M0102635, UL1RR02575801]; New Balance Foundation; foundations for obesity-related research, mentoring, and patient care FX All authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Dr Ebbeling reported receiving grants from the National Institutes of Health and foundations for obesity-related research. Dr Ludwig reported receiving grants from the National Institutes of Health and foundations for obesity-related research, mentoring, and patient care; and royalties from a book about childhood obesity. No other authors reported any conflicts of interest.; This study was supported by grants R01DK072428 and K24DK082730 from the National Institute of Diabetes and Digestive and Kidney Diseases, Bethesda, Maryland; grant M01RR02172 from the National Center for Research Resources, National Institutes of Health, to the Children's Hospital Boston General Clinical Research Center; grant M0102635 from the National Center for Research Resources, National Institutes of Health, to the Brigham and Women's Hospital General Clinical Research Center; grant UL1RR02575801 from the National Center for Research Resources, National Institutes of Health, to the Harvard Catalyst Clinical and Translational Science Center; and a grant from the New Balance Foundation. NR 44 TC 109 Z9 109 U1 3 U2 53 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 0098-7484 EI 1538-3598 J9 JAMA-J AM MED ASSOC JI JAMA-J. Am. Med. Assoc. PD JUN 27 PY 2012 VL 307 IS 24 BP 2627 EP 2634 DI 10.1001/jama.2012.6607 PG 8 WC Medicine, General & Internal SC General & Internal Medicine GA 964KF UT WOS:000305692600031 PM 22735432 ER PT J AU Ali, JG Alborn, HT Campos-Herrera, R Kaplan, F Duncan, LW Rodriguez-Saona, C Koppenhofer, AM Stelinski, LL AF Ali, Jared G. Alborn, Hans T. Campos-Herrera, Raquel Kaplan, Fatma Duncan, Larry W. Rodriguez-Saona, Cesar Koppenhoefer, Albrecht M. Stelinski, Lukasz L. TI Subterranean, Herbivore-Induced Plant Volatile Increases Biological Control Activity of Multiple Beneficial Nematode Species in Distinct Habitats SO PLOS ONE LA English DT Article ID DIAPREPES-ABBREVIATUS COLEOPTERA; TIME PCR ASSAYS; ENTOMOPATHOGENIC NEMATODES; FLORIDA CITRUS; PARASITIC NEMATODES; TROPHIC CASCADE; SOIL; ROOTS; CURCULIONIDAE; INSECTS AB While the role of herbivore-induced volatiles in plant-herbivore-natural enemy interactions is well documented aboveground, new evidence suggests that belowground volatile emissions can protect plants by attracting entomopathogenic nematodes (EPNs). However, due to methodological limitations, no study has previously detected belowground herbivore-induced volatiles in the field or quantified their impact on attraction of diverse EPN species. Here we show how a belowground herbivore-induced volatile can enhance mortality of agriculturally significant root pests. First, in real time, we identified pregeijerene (1,5-dimethylcyclodeca-1,5,7-triene) from citrus roots 9-12 hours after initiation of larval Diaprepes abbreviatus feeding. This compound was also detected in the root zone of mature citrus trees in the field. Application of collected volatiles from weevil-damaged citrus roots attracted native EPNs and increased mortality of beetle larvae (D. abbreviatus) compared to controls in a citrus orchard. In addition, field applications of isolated pregeijerene caused similar results. Quantitative real-time PCR revealed that pregeijerene increased pest mortality by attracting four species of naturally occurring EPNs in the field. Finally, we tested the generality of this root-zone signal by application of pregeijerene in blueberry fields; mortality of larvae (Galleria mellonella and Anomala orientalis) again increased by attracting naturally occurring populations of an EPN. Thus, this specific belowground signal attracts natural enemies of widespread root pests in distinct agricultural systems and may have broad potential in biological control of root pests. C1 [Ali, Jared G.; Campos-Herrera, Raquel; Duncan, Larry W.; Stelinski, Lukasz L.] Univ Florida, Ctr Citrus Res & Educ, Dept Entomol & Nematol, Lake Alfred, FL USA. [Alborn, Hans T.; Kaplan, Fatma] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. [Campos-Herrera, Raquel] Ctr Ciencias Medioambientales, Inst Ciencias Agr, Dept Contaminac Ambiental, Madrid, Spain. [Rodriguez-Saona, Cesar; Koppenhoefer, Albrecht M.] Rutgers State Univ, Dept Entomol, New Brunswick, NJ 08903 USA. RP Ali, JG (reprint author), Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA. EM stelinski@ufl.edu RI Raquel, Campos Herrera/G-5285-2012; Campos-Herrera, Raquel/A-5299-2017; OI Campos-Herrera, Raquel/0000-0003-0852-5269; Ali, Jareg/0000-0002-5794-9554 FU USDA-CSREES; Citrus Research and Development Foundation; University of Florida; European Union [FP7-PEOLE-2009-10F-252980] FX This research was partially supported by grants from the USDA-CSREES, Citrus Research and Development Foundation and start up funding provided by the University of Florida to LLS. RCH was supported by the Seventh Framework Programme of the European Union (FP7-PEOLE-2009-10F-252980). Funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 59 TC 26 Z9 27 U1 3 U2 48 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 27 PY 2012 VL 7 IS 6 AR e38146 DI 10.1371/journal.pone.0038146 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 966GL UT WOS:000305825800009 PM 22761668 ER PT J AU Galeano, CH Cortes, AJ Fernandez, AC Soler, A Franco-Herrera, N Makunde, G Vanderleyden, J Blair, MW AF Galeano, Carlos H. Cortes, Andres J. Fernandez, Andrea C. Soler, Alvaro Franco-Herrera, Natalia Makunde, Godwill Vanderleyden, Jos Blair, Matthew W. TI Gene-Based Single Nucleotide Polymorphism Markers for Genetic and Association Mapping in Common Bean SO BMC GENETICS LA English DT Article ID GENOME-WIDE ASSOCIATION; PHASEOLUS-VULGARIS L.; QUANTITATIVE TRAIT LOCI; AUXIN RESPONSE FACTORS; LINKAGE DISEQUILIBRIUM; POPULATION-STRUCTURE; SNP DISCOVERY; ARABIDOPSIS-THALIANA; MEDICAGO-TRUNCATULA; FUNCTIONAL GENOMICS AB Background: In common bean, expressed sequence tags (ESTs) are an underestimated source of gene-based markers such as insertion-deletions (Indels) or single-nucleotide polymorphisms (SNPs). However, due to the nature of these conserved sequences, detection of markers is difficult and portrays low levels of polymorphism. Therefore, development of intron-spanning EST-SNP markers can be a valuable resource for genetic experiments such as genetic mapping and association studies. Results: In this study, a total of 313 new gene-based markers were developed at target genes. Intronic variation was deeply explored in order to capture more polymorphism. Introns were putatively identified after comparing the common bean ESTs with the soybean genome, and the primers were designed over intron-flanking regions. The intronic regions were evaluated for parental polymorphisms using the single strand conformational polymorphism (SSCP) technique and Sequenom MassARRAY system. A total of 53 new marker loci were placed on an integrated molecular map in the DOR364 x G19833 recombinant inbred line (RIL) population. The new linkage map was used to build a consensus map, merging the linkage maps of the BAT93 x JALO EEP558 and DOR364 x BAT477 populations. A total of 1,060 markers were mapped, with a total map length of 2,041 cM across 11 linkage groups. As a second application of the generated resource, a diversity panel with 93 genotypes was evaluated with 173 SNP markers using the MassARRAY-platform and KASPar technology. These results were coupled with previous SSR evaluations and drought tolerance assays carried out on the same individuals. This agglomerative dataset was examined, in order to discover marker-trait associations, using general linear model (GLM) and mixed linear model (MLM). Some significant associations with yield components were identified, and were consistent with previous findings. Conclusions: In short, this study illustrates the power of intron-based markers for linkage and association mapping in common bean. The utility of these markers is discussed in relation with the usefulness of microsatellites, the molecular markers by excellence in this crop. C1 [Galeano, Carlos H.] Ctr Microbial & Plant Genet, B-3001 Heverlee, Belgium. [Cortes, Andres J.] Uppsala Univ, Evolutionary Biol Ctr, SE-75105 Uppsala, Sweden. [Fernandez, Andrea C.] USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI USA. [Soler, Alvaro; Franco-Herrera, Natalia] Ctr Int Agr Trop, Bean Project, Cali 6713, Colombia. [Makunde, Godwill] Crops Breeding Inst, Harare, Zimbabwe. RP Galeano, CH (reprint author), Ctr Microbial & Plant Genet, Kasteelpk Arenberg 20, B-3001 Heverlee, Belgium. EM galeanomendoza@gmail.com; mwb1@cornell.edu FU IRO (Interfaculty Council for Development Co-operation of the Catholic University of Leuven) FX We are grateful to F. Monserrate and J.C. Perez for trial management and data collection. We would also like to thank the anonymous reviewers for their thoughtful comments and suggestions to improve the manuscript. CHG is supported by a doctoral research fellowship from IRO (Interfaculty Council for Development Co-operation of the Catholic University of Leuven). NR 77 TC 23 Z9 26 U1 1 U2 51 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUN 26 PY 2012 VL 13 AR 48 DI 10.1186/1471-2156-13-48 PG 11 WC Genetics & Heredity SC Genetics & Heredity GA 006OU UT WOS:000308829300001 PM 22734675 ER PT J AU Wu, YY Li, XR Xiang, WW Zhu, CS Lin, ZW Wu, Y Li, JR Pandravada, S Ridder, DD Bai, GH Wang, ML Trick, HN Bean, SR Tuinstra, MR Tesso, TT Yu, JM AF Wu, Yuye Li, Xianran Xiang, Wenwen Zhu, Chengsong Lin, Zhongwei Wu, Yun Li, Jiarui Pandravada, Satchidanand Ridder, Dustan D. Bai, Guihua Wang, Ming L. Trick, Harold N. Bean, Scott R. Tuinstra, Mitchell R. Tesso, Tesfaye T. Yu, Jianming TI Presence of tannins in sorghum grains is conditioned by different natural alleles of Tannin1 SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE domestication; food production; gene cloning; health benefit; natural selection ID QUANTITATIVE TRAIT LOCI; ARABIDOPSIS-THALIANA; PROANTHOCYANIDINS; BIOSYNTHESIS; PROTEIN; METAANALYSIS; COMMUNITY; GENETICS; ENCODES; BANYULS AB Sorghum, an ancient old-world cereal grass, is the dietary staple of over 500 million people in more than 30 countries in the tropics and semitropics. Its C4 photosynthesis, drought resistance, wide adaptation, and high nutritional value hold the promise to alleviate hunger in Africa. Not present in other major cereals, such as rice, wheat, and maize, condensed tannins (proanthocyanidins) in the pigmented testa of some sorghum cultivars have been implicated in reducing protein digestibility but recently have been shown to promote human health because of their high antioxidant capacity and ability to fight obesity through reduced digestion. Combining quantitative trait locus mapping, meta-quantitative trait locus fine-mapping, and association mapping, we showed that the nucleotide polymorphisms in the Tan1 gene, coding a WD40 protein, control the tannin biosynthesis in sorghum. A 1-bp G deletion in the coding region, causing a frame shift and a premature stop codon, led to a nonfunctional allele, tan1-a. Likewise, a different 10-bp insertion resulted in a second nonfunctional allele, tan1-b. Transforming the sorghum Tan1 ORF into a nontannin Arabidopsis mutant restored the tannin phenotype. In addition, reduction in nucleotide diversity from wild sorghum accessions to landraces and cultivars was found at the region that codes the highly conserved WD40 repeat domains and the C-terminal region of the protein. Genetic research in crops, coupled with nutritional and medical research, could open the possibility of producing different levels and combinations of phenolic compounds to promote human health. C1 [Wu, Yuye; Li, Xianran; Xiang, Wenwen; Zhu, Chengsong; Lin, Zhongwei; Wu, Yun; Pandravada, Satchidanand; Ridder, Dustan D.; Bai, Guihua; Tesso, Tesfaye T.; Yu, Jianming] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Li, Jiarui; Trick, Harold N.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Bai, Guihua] USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Wang, Ming L.] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Bean, Scott R.] USDA ARS, Grain Qual & Struct Res Unit, Manhattan, KS 66502 USA. [Tuinstra, Mitchell R.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Yu, JM (reprint author), Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. EM jyu@ksu.edu RI Li, Xianran/I-5651-2012; OI Li, Xianran/0000-0002-4252-6911; Yu, Jianming/0000-0001-5326-3099; Trick, Harold/0000-0001-5255-5575 FU US Department of Agriculture National Institute of Food and Agriculture [2011-03587]; US Department of Energy [DE-SC0002259]; National Science Foundation [DBI-0820610]; US Department of Agriculture-Agricultural Research Service; National Sorghum Checkoff program FX We thank Dr. Lloyd Rooney for providing information on food and feed quality attributes of sorghum. This work was supported by Agriculture and Food Research Initiative Competitive Grant 2011-03587 from the US Department of Agriculture National Institute of Food and Agriculture; the Plant Feedstock Genomics Program (DE-SC0002259) of the US Department of Energy; and the Plant Genome Program (DBI-0820610) of the National Science Foundation, the US Department of Agriculture-Agricultural Research Service, and the National Sorghum Checkoff program. NR 34 TC 10 Z9 12 U1 1 U2 29 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 26 PY 2012 VL 109 IS 26 BP 10281 EP 10286 DI 10.1073/pnas.1201700109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 972QE UT WOS:000306291400044 PM 22699509 ER PT J AU Ament, SA Blatti, CA Alaux, C Wheeler, MM Toth, AL Le Conte, Y Hunte, GJ Guzman-Novoaf, E DeGrandi-Hoffman, G Uribe-Rubio, JL Amdam, GV Page, RE Rodriguez-Zas, SL Robinson, GE Sinha, S AF Ament, Seth A. Blatti, Charles A. Alaux, Cedric Wheeler, Marsha M. Toth, Amy L. Le Conte, Yves Hunte, Greg J. Guzman-Novoaf, Ernesto DeGrandi-Hoffman, Gloria Uribe-Rubio, Jose Luis Amdam, Gro V. Page, Robert E., Jr. Rodriguez-Zas, Sandra L. Robinson, Gene E. Sinha, Saurabh TI New meta-analysis tools reveal common transcriptional regulatory basis for multiple determinants of behavior SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE honey bee; transcriptional regulation ID DIVISION-OF-LABOR; GENE-EXPRESSION PROFILES; HONEYBEE APIS-MELLIFERA; HUMAN CORE PROMOTERS; ENRICHMENT ANALYSIS; CANCER MICROARRAY; SOCIAL-BEHAVIOR; GENOME; BEE; INTEGRATION AB A fundamental problem in meta-analysis is how to systematically combine information from multiple statistical tests to rigorously evaluate a single overarching hypothesis. This problem occurs in systems biology when attempting to map genomic attributes to complex phenotypes such as behavior. Behavior and other complex phenotypes are influenced by intrinsic and environmental determinants that act on the transcriptome, but little is known about how these determinants interact at the molecular level. We developed an informatic technique that identifies statistically significant meta-associations between gene expression patterns and transcription factor combinations. Deploying this technique for brain transcriptome profiles from ca. 400 individual bees, we show that diverse determinants of behavior rely on shared combinations of transcription factors. These relationships were revealed only when we considered complex and variable regulatory rules, suggesting that these shared transcription factors are used in distinct ways by different determinants. This regulatory code would have been missed by traditional gene coexpression or cis-regulatory analytic methods. We expect that our meta-analysis tools will be useful for a broad array of problems in systems biology and other fields. C1 [Ament, Seth A.; Robinson, Gene E.] Univ Illinois, Neurosci Program, Urbana, IL 61801 USA. [Blatti, Charles A.; Sinha, Saurabh] Univ Illinois, Dept Comp Sci, Urbana, IL 61801 USA. [Alaux, Cedric; Wheeler, Marsha M.; Toth, Amy L.; Robinson, Gene E.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Rodriguez-Zas, Sandra L.] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA. [Rodriguez-Zas, Sandra L.; Robinson, Gene E.; Sinha, Saurabh] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Alaux, Cedric; Le Conte, Yves] INRA, Unite Rech UR Abeilles & Environm 406, Avignon 9, France. [Hunte, Greg J.] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Guzman-Novoaf, Ernesto] Univ Guelph, Dept Environm Biol, Guelph, ON N1G 2W1, Canada. [DeGrandi-Hoffman, Gloria] ARS, Carl Hayden Bee Res Ctr, USDA, Tucson, AZ 85719 USA. [Uribe-Rubio, Jose Luis] Inst Nacl Invest Forestales Agr & Pecuarias, Ctr Nacl Invest Fisiol Anim, Ajuchitlan 76280, Qro, Mexico. [Amdam, Gro V.; Page, Robert E., Jr.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Amdam, Gro V.] Norwegian Univ Life Sci, Dept Chem Biotechnol & Food Sci, N-1432 As, Norway. RP Robinson, GE (reprint author), Univ Illinois, Neurosci Program, Urbana, IL 61801 USA. EM generobi@illinois.edu; sinhas@illinois.edu RI Alaux, Cedric/B-4667-2013; Le Conte, Yves/B-3246-2016 FU National Science Foundation [EF25852, DBI-0746303]; National Institutes of Health [1DP1OD006416, 1R01DK082605-01A1, 1R01GM085233-01] FX We thank K. S. Pruiett for beekeeping; T. C. Newman for assistance with molecular techniques; C. J. Fields for mapping cDNA array features to the oligo array; J. J. Collins, V. Jongeneel, J. Ma, P. Ma, N.D. Price, L. J. Stubbs, and S. Zhong for reviewing the manuscript; E. Carefoot for producing the bee cartoons; and O. Georgiou for sharing a C routine used in our implementation of meta-P values. Funding was from National Science Foundation Frontiers in Biological Research Award EF25852 (B. Schatz, principal investigator); National Institutes of Health Director's Pioneer Awards 1DP1OD006416 (to G. E. R.), 1R01DK082605-01A1 (to G. E. R.), and 1R01GM085233-01 (to S. S.); and National Science Foundation Grant DBI-0746303 (to S. S.). Table S4 shows accession numbers for each experiment (http://www.ebi.ac.uk/microarray-as/ae/). NR 64 TC 26 Z9 26 U1 0 U2 52 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 26 PY 2012 VL 109 IS 26 BP E1801 EP E1810 DI 10.1073/pnas.1205283109 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 972QE UT WOS:000306291400017 PM 22691501 ER PT J AU Parks, M Cronn, R Liston, A AF Parks, Matthew Cronn, Richard Liston, Aaron TI Separating the wheat from the chaff: mitigating the effects of noise in a plastome phylogenomic data set from Pinus L. (Pinaceae) SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Phylogenetic noise; Plastome; Pinus; Chloroplast ID LONG-BRANCH ATTRACTION; CHLOROPLAST GENOME SEQUENCES; LENGTH DIFFERENCE TEST; PINES PINUS; MOLECULAR PHYLOGENY; SUBGENUS PINUS; ENRICHMENT STRATEGIES; MAXIMUM-LIKELIHOOD; EVOLUTIONARY TREES; SYSTEMATIC BIASES AB Background: Through next-generation sequencing, the amount of sequence data potentially available for phylogenetic analyses has increased exponentially in recent years. Simultaneously, the risk of incorporating 'noisy' data with misleading phylogenetic signal has also increased, and may disproportionately influence the topology of weakly supported nodes and lineages featuring rapid radiations and/or elevated rates of evolution. Results: We investigated the influence of phylogenetic noise in large data sets by applying two fundamental strategies, variable site removal and long-branch exclusion, to the phylogenetic analysis of a full plastome alignment of 107 species of Pinus and six Pinaceae outgroups. While high overall phylogenetic resolution resulted from inclusion of all data, three historically recalcitrant nodes remained conflicted with previous analyses. Close investigation of these nodes revealed dramatically different responses to data removal. Whereas topological resolution and bootstrap support for two clades peaked with removal of highly variable sites, the third clade resolved most strongly when all sites were included. Similar trends were observed using long-branch exclusion, but patterns were neither as strong nor as clear. When compared to previous phylogenetic analyses of nuclear loci and morphological data, the most highly supported topologies seen in Pinus plastome analysis are congruent for the two clades gaining support from variable site removal and long-branch exclusion, but in conflict for the clade with highest support from the full data set. Conclusions: These results suggest that removal of misleading signal in phylogenomic datasets can result not only in increased resolution for poorly supported nodes, but may serve as a tool for identifying erroneous yet highly supported topologies. For Pinus chloroplast genomes, removal of variable sites appears to be more effective than long-branch exclusion for clarifying phylogenetic hypotheses. C1 [Parks, Matthew; Liston, Aaron] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Cronn, Richard] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP Parks, M (reprint author), Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. EM parksma@science.oregonstate.edu FU National Science Foundation grant [NSF ATOL-0629508] FX This work was supported by the National Science Foundation grant number NSF ATOL-0629508. NR 114 TC 45 Z9 47 U1 4 U2 43 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD JUN 25 PY 2012 VL 12 AR 100 DI 10.1186/1471-2148-12-100 PG 17 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA 023XJ UT WOS:000310070600001 PM 22731878 ER PT J AU Kuykendall, LD Shao, JY Hartung, JS AF Kuykendall, L. David Shao, Jonathan Y. Hartung, John S. TI 'Ca. Liberibacter asiaticus' Proteins Orthologous with pSymA-Encoded Proteins of Sinorhizobium meliloti: Hypothetical Roles in Plant Host Interaction SO PLOS ONE LA English DT Article ID ESCHERICHIA-COLI; GENOME SEQUENCE; LYSR HOMOLOG; BACTERIA; EFFLUX; ANTIPORTER; EXPRESSION; MECHANISM; MOTILITY; FAMILY AB Sinorhizobium meliloti strain 1021, a nitrogen-fixing, root-nodulating bacterial microsymbiont of alfalfa, has a 3.5 Mbp circular chromosome and two megaplasmids including 1.3 Mbp pSymA carrying nonessential 'accessory' genes for nitrogen fixation (nif), nodulation and host specificity (nod). A related bacterium, psyllid-vectored 'Ca. Liberibacter asiaticus,' is an obligate phytopathogen with a reduced genome that was previously analyzed for genes orthologous to genes on the S. meliloti circular chromosome. In general, proteins encoded by pSymA genes are more similar in sequence alignment to those encoded by S. meliloti chromosomal orthologs than to orthologous proteins encoded by genes carried on the 'Ca. Liberibacter asiaticus' genome. Only two 'Ca. Liberibacter asiaticus' proteins were identified as having orthologous proteins encoded on pSymA but not also encoded on the chromosome of S. meliloti. These two orthologous gene pairs encode a Na+/K+ antiporter (shared with intracellular pathogens of the family Bartonellacea) and a Co++, Zn++ and Cd++ cation efflux protein that is shared with the phytopathogen Agrobacterium. Another shared protein, a redox-regulated K+ efflux pump may regulate cytoplasmic pH and homeostasis. The pSymA and 'Ca. Liberibacter asiaticus' orthologs of the latter protein are more highly similar in amino acid alignment compared with the alignment of the pSymA-encoded protein with its S. meliloti chromosomal homolog. About 182 pSymA encoded proteins have sequence similarity (<= E-10) with 'Ca. Liberibacter asiaticus' proteins, often present as multiple orthologs of single 'Ca. Liberibacter asiaticus' proteins. These proteins are involved with amino acid uptake, cell surface structure, chaperonins, electron transport, export of bioactive molecules, cellular homeostasis, regulation of gene expression, signal transduction and synthesis of amino acids and metabolic cofactors. The presence of multiple orthologs defies mutational analysis and is consistent with the hypothesis that these proteins may be of particular importance in host/microbe interaction and their duplication likely facilitates their ongoing evolution. C1 [Kuykendall, L. David; Shao, Jonathan Y.; Hartung, John S.] ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD USA. RP Kuykendall, LD (reprint author), ARS, USDA, Mol Plant Pathol Lab, Beltsville, MD USA. EM John.Hartung@ars.usda.gov NR 39 TC 3 Z9 3 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 25 PY 2012 VL 7 IS 6 AR e38725 DI 10.1371/journal.pone.0038725 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 965QG UT WOS:000305781700010 PM 22761700 ER PT J AU Guillaumet, A Dorr, B Wang, GM AF Guillaumet, Alban Dorr, Brian Wang, Guiming TI Towards optimized population control efficiency in space and time: A modelling framework adapted to a colonial waterbird SO ECOLOGICAL MODELLING LA English DT Article DE Adaptive management; Human-wildlife conflict; Management efficiency; Metapopulation; Model; Simulation ID DOUBLE-CRESTED CORMORANTS; DENSITY-DEPENDENCE; GREAT CORMORANT; DISPERSAL; MANAGEMENT; RECRUITMENT; BIRD AB The double-crested cormorant is a native North American waterbird that recently underwent a dramatic population expansion. Population control efforts in the USA and Canada attempt to mitigate cormorant damages to natural resources and aquaculture. However, there is currently no coordination among the various stakeholders involved in management activities as well as no attempt to optimize population control efficiency. In this paper, we present for the first time a spatially explicit stage-structured metapopulation model parameterized for the cormorant. We developed simulation tools to get insights into the efficiency gain that can be expected from a better planning of management activities in both space and time. A case study is presented, in which we randomized where (on which colonies) and when (which years) a pre-determined amount of management activities would occur on 4 or 8 of 16 active colonies arranged on a 4 x 4 or 2 x 8 spatial grid over a period of 8 years, including 2 or 4 management years. We calculated two indices measuring the location of management activities, namely the average date of management years and the average degree of peripherality of colonies undertaking management, together with two indices measuring the resulting correlation of management activities, i.e., the degree of clustering of management activities both in space and time. Different spatio-temporal configurations of management activities generally yielded different metapopulation trajectories. Room for improving management efficiency increased with the intensity of management activities. However, the greatest efficiency gains are to be expected when colonies are far from carrying capacity, while the majority of management operations are undertaken when colonies are near or at carrying capacity. Locations of management activities in space and time appeared more important than resulting spatio-temporal correlations to explain the dispersion of metapopulation trajectories. When colonies were far from their individual carrying capacity, management was more efficient when applied earlier (a consequence of delayed reproductive maturity) and to more central colonies (due to greater immigration). The situation was more complex when colonies were closer to or at carrying capacity. Our modelling framework is flexible enough to allow more complex scenarios to be investigated in the future. (C) 2012 Elsevier B.V. All rights reserved. C1 [Guillaumet, Alban] Trent Univ, Dept Biol, Off D223, Peterborough, ON K9J 7B8, Canada. [Guillaumet, Alban; Wang, Guiming] Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. [Dorr, Brian] Natl Wildlife Res Ctr, USDA, Wildlife Serv, Mississippi State, MS 39762 USA. RP Guillaumet, A (reprint author), Trent Univ, Dept Biol, Off D223, DNA Bldg, Peterborough, ON K9J 7B8, Canada. EM albanguillaumet@trentu.ca OI Dorr, Brian/0000-0001-6857-8560 FU USDA Wildlife Services; National Wildlife Research Center; Jack. H. Berryman Institute (Eastern Unit); Forest and Wildlife Research Center, Mississippi State University FX We are grateful to three anonymous reviewers for their constructive comments on our manuscript. This project was funded by the USDA Wildlife Services, National Wildlife Research Center, and the Jack. H. Berryman Institute (Eastern Unit). G.M. Wang was partially supported by the Forest and Wildlife Research Center, Mississippi State University. NR 26 TC 1 Z9 1 U1 1 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 J9 ECOL MODEL JI Ecol. Model. PD JUN 24 PY 2012 VL 235 BP 95 EP 101 DI 10.1016/j.ecolmodel.2012.03.028 PG 7 WC Ecology SC Environmental Sciences & Ecology GA 960FD UT WOS:000305372500010 ER PT J AU Stone, CL McMahon, MB Fortis, LL Nunez, A Smythers, GW Luster, DG Frederick, RD AF Stone, Christine L. McMahon, Michael B. Fortis, Laurie L. Nunez, Alberto Smythers, Gary W. Luster, Douglas G. Frederick, Reid D. TI Gene expression and proteomic analysis of the formation of Phakopsora pachyrhizi appressoria SO BMC GENOMICS LA English DT Article ID RICE BLAST FUNGUS; MEDIATED PLANT INFECTION; SUPPRESSION SUBTRACTIVE HYBRIDIZATION; POLYMERASE-CHAIN-REACTION; ASIAN SOYBEAN RUST; MAGNAPORTHE-GRISEA; COLLETOTRICHUM-LAGENARIUM; SUBCELLULAR-LOCALIZATION; CELLULAR-DIFFERENTIATION; PHYTOPHTHORA-INFESTANS AB Background: Phakopsora pachyrhizi is an obligate fungal pathogen causing Asian soybean rust (ASR). A dual approach was taken to examine the molecular and biochemical processes occurring during the development of appressoria, specialized infection structures by which P. pachyrhizi invades a host plant. Suppression subtractive hybridization (SSH) was utilized to generate a cDNA library enriched for transcripts expressed during appressoria formation. Two-dimensional gel electrophoresis and mass spectroscopy analysis were used to generate a partial proteome of proteins present during appressoria formation. Results: Sequence analysis of 1133 expressed sequence tags (ESTs) revealed 238 non-redundant ESTs, of which 53% had putative identities assigned. Twenty-nine of the non-redundant ESTs were found to be specific to the appressoria-enriched cDNA library, and did not occur in a previously constructed germinated urediniospore cDNA library. Analysis of proteins against a custom database of the appressoria-enriched ESTs plus Basidiomycota EST sequences available from NCBI revealed 256 proteins. Fifty-nine of these proteins were not previously identified in a partial proteome of P. pachyrhizi germinated urediniospores. Genes and proteins identified fell into functional categories of metabolism, cell cycle and DNA processing, protein fate, cellular transport, cellular communication and signal transduction, and cell rescue. However, 38% of ESTs and 24% of proteins matched only to hypothetical proteins of unknown function, or showed no similarity to sequences in the current NCBI database. Three novel Phakopsora genes were identified from the cDNA library along with six potentially rust-specific genes. Protein analysis revealed eight proteins of unknown function, which possessed classic secretion signals. Two of the extracellular proteins are reported as potential effector proteins. Conclusions: Several genes and proteins were identified that are expressed in P. pachyrhizi during appressoria formation. Understanding the role that these genes and proteins play in the molecular and biochemical processes in the infection process may provide insight for developing targeted control measures and novel methods of disease management. C1 [Stone, Christine L.; McMahon, Michael B.; Luster, Douglas G.; Frederick, Reid D.] Agr Res Serv, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Fortis, Laurie L.; Nunez, Alberto] Agr Res Serv, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Smythers, Gary W.] NCI, Adv Biomed Comp Ctr, Ft Detrick, MD 21702 USA. RP Frederick, RD (reprint author), Agr Res Serv, USDA, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM reid.frederick@ars.usda.gov FU USDA CRIS project [1920-22000-035-00D] FX This research was supported by USDA CRIS project 1920-22000-035-00D. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The U.S. Department of Agriculture (USDA) prohibits discrimination in all its programs and activities on the basis of race, color, national origin, age, disability, and where applicable, sex, marital status, familial status, parental status, religion, sexual orientation, genetic information, political beliefs, reprisal, or because all or part of an individual's income is derived from any public assistance program. (Not all prohibited bases apply to all programs.) Persons with disabilities who require alternative means for communication of program information (Braille, large print, audiotape, etc.) should contact USDA's TARGET Center at (202) 720-2600 (voice and TDD). To file a complaint of discrimination, write to USDA, Director, Office of Civil Rights, 1400 Independence Avenue, S.W., Washington, D.C. 20250-9410, or call (800) 795-3272 (voice) or (202) 720-6382 (TDD). USDA is an equal opportunity provider and employer. NR 79 TC 5 Z9 5 U1 2 U2 25 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 22 PY 2012 VL 13 AR 269 DI 10.1186/1471-2164-13-269 PG 21 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 000CW UT WOS:000308365100001 PM 22727213 ER PT J AU Green, BW Rawles, SD Beck, BH AF Green, Bartholomew W. Rawles, Steven D. Beck, Benjamin H. TI Response of channel x blue hybrid catfish to chronic diurnal hypoxia SO AQUACULTURE LA English DT Article DE Channel x blue hybrid catfish; Hypoxia; Dissolved oxygen; Pond production; Body compositional indices; Citrate synthase ID DISSOLVED-OXYGEN CONCENTRATIONS; EARTHEN PONDS; ICTALURUS-PUNCTATUS; WATER TEMPERATURE; FEED-EFFICIENCY; GROWTH; DENSITIES; YIELD; FISH; METABOLISM AB Performance traits and metabolic responses of the channel x blue hybrid catfish (Ictalurus punctatus female x I. furcatus male) in response to chronic diurnal hypoxia were evaluated in this 197-d study. Sixteen 0.1-ha earthen ponds were stocked with 15,169 hybrid catfish/ha (47 g/fish) and managed to maintain the minimum dissolved oxygen concentration greater than 12%, 24%, 36%, or 48% of saturation. Growth and yield of channel x blue hybrid catfish were significantly related to minimum nightly dissolved oxygen concentration. The cumulative effect of nocturnal dissolved oxygen concentration and the duration of exposure to hypoxia (termed dissolved oxygen-minutes) proved to be a better independent variable for regression analysis than minimum nightly dissolved oxygen concentration. Gross and net yield and mean individual weight increased curvilinearly as dissolved oxygen-minutes increased. Chronic nightly hypoxia affected daily feed consumption and channel x blue hybrid catfish in the higher dissolved oxygen treatments grew faster because they consumed a greater percentage of their body weight at each feeding. Feed consumption increased linearly in response to dissolved oxygen-minutes during the peak production period (June-August), but curvilinearly over the entire study. Body compositional indices largely were unaffected by chronic nightly hypoxia. Lipid was the primary depot affected by hypoxia and lipid indices increased with increasing dissolved oxygen concentration and with increasing total feed fed. Citrate synthase activity was 14.6% higher in the highest compared to the lowest dissolved oxygen treatment and a strong inverse relationship between citrate synthase activity and fish body mass was observed in the highest dissolved oxygen concentration treatment. Results of this study suggest that pond dissolved oxygen concentration should be maintained at 48% saturation during the peak production period (water temperatures above 25 C) and at 36% saturation during the rest of the growing season. Published by Elsevier B.V. C1 [Green, Bartholomew W.; Rawles, Steven D.; Beck, Benjamin H.] USDA Agr Res Serv, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Green, BW (reprint author), USDA Agr Res Serv, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. EM bart.green@ars.usda.gov; steven.rawles@ars.usda.gov; benjamin.beck@ars.usda.gov FU USDA/ARS [6225-31630-006-00D] FX We thank Greg O'Neal and Rebecca Jacobs for their assistance throughout this study, Dr. Adam Fuller, Matt McEntire, and Bradley Farmer for their assistance with tissue sample collection, and our many co-workers at the Stuttgart National Aquaculture Research Center who assisted during harvest. We also thank the UCDC DRTC BARB Core (P60 DK079626) at the University of Alabama at Birmingham for examining citrate synthase activity. This study was funded by USDA/ARS under project number 6225-31630-006-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 44 TC 2 Z9 2 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 J9 AQUACULTURE JI Aquaculture PD JUN 20 PY 2012 VL 350 BP 183 EP 191 DI 10.1016/j.aquaculture.2012.03.041 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA 956DA UT WOS:000305068800026 ER PT J AU Casasnovas, JA Alcaide, V Civeira, F Guallar, E Ibanez, B Borreguero, JJ Laclaustra, M Leon, M Penalvo, JL Ordovas, JM Pocovi, M Sanz, G Fuster, V AF Casasnovas, Jose A. Alcaide, Victor Civeira, Fernando Guallar, Eliseo Ibanez, Borja Jimenez Borreguero, Jesus Laclaustra, Martin Leon, Montserrat Luis Penalvo, Jose Ordovas, Jose M. Pocovi, Miguel Sanz, Gines Fuster, Valentin TI Aragon workers' health study - design and cohort description SO BMC CARDIOVASCULAR DISORDERS LA English DT Article ID CARDIOVASCULAR RISK-FACTORS; INTIMA-MEDIA THICKNESS; MONICA PROJECT POPULATIONS; SPANISH WORKING POPULATION; CORONARY-HEART-DISEASE; CONSENSUS STATEMENT; AMERICAN SOCIETY; EVENT RATES; TASK-FORCE; PREVALENCE AB Background: Spain, a Mediterranean country with relatively low rates of coronary heart disease, has a high prevalence of traditional cardiovascular risk factors and is experiencing a severe epidemic of overweight/obesity. We designed the Aragon Workers' Health Study (AWHS) to characterize the factors associated with metabolic abnormalities and subclinical atherosclerosis in a middle aged population in Spain free of clinical cardiovascular disease. The objective of this paper is to describe the study design, aims and baseline characteristics of participants in the AWHS. Methods/Design: Longitudinal cohort study based on the annual health exams of 5,400 workers of a car assembly plant in Figueruelas (Zaragoza, Spain). Study participants were recruited during a standardized clinical exam in 2009-2010 (participation rate 95.6%). Study participants will undergo annual clinical exams and laboratory assays, and baseline and triennial collection of biological materials for biobanking and cardiovascular imaging exams (carotid, femoral and abdominal ultrasonography, coronary calcium score, and ankle-arm blood pressure index). Participants will be followed-up for 10 years. Results: The average (SD) age, body mass index, and waist circumference were 49.3 (8.7) years, 27.7 (3.6) kg/m(2) and 97.2 (9.9) cm, respectively, among males (N = 5,048), and 40.8 (11.6) years, 24.4 (3.8) kg/m(2), and 81.9 (9.9) cm, among females (N = 351). The prevalence of overweight, obesity, current smoking, hypertension, hypercholesterolemia, and diabetes were 55.0, 23.1, 37.1, 40.3, 75.0, and 7.4%, respectively, among males, and 23.7, 8.3, 45.0, 12.1, 59.5, and 0.6%, respectively, among females. In the initial 587 study participants who completed all imaging exams (94.5% male), the prevalence of carotid plaque, femoral plaque, coronary calcium score >1 to 100, and coronary calcium score >100 was 30.3, 56.9, 27.0, and 8.8%, respectively. 67.7% of study participants had at least one plaque in the carotid or femoral arteries. Discussion: Baseline data from the AWHS show a high prevalence of cardiovascular risk factors and of sublinical atherosclerosis. Follow-up of this cohort will allow the assessment of subclinical atherosclerosis progression and the link of disease progression to traditional and emergent risk factors. C1 [Guallar, Eliseo; Ibanez, Borja; Jimenez Borreguero, Jesus; Laclaustra, Martin; Luis Penalvo, Jose; Ordovas, Jose M.; Sanz, Gines; Fuster, Valentin] Natl Ctr Cardiovasc Res CNIC, Dept Epidemiol Atherothrombosis & Imaging, Madrid, Spain. [Casasnovas, Jose A.; Leon, Montserrat] Inst Aragones Ciencias Salud I CS, Cardiovasc Res Unit, Zaragoza, Spain. [Alcaide, Victor] Gen Motors Spain, Prevent Depatment, Figueruelas, Spain. [Civeira, Fernando] Hosp Univ Miguel Servet, Inst Aragones Ciencias Salud I CS, Lipids Unit, Zaragoza, Spain. [Civeira, Fernando] Hosp Univ Miguel Servet, Inst Aragones Ciencias Salud I CS, Mol Res Lab, Zaragoza, Spain. [Guallar, Eliseo] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Welch Ctr Prevent Epidemiol & Clin Res, Baltimore, MD 21205 USA. [Ibanez, Borja] Hosp Clin San Carlos, Cardiovasc Inst, Madrid, Spain. [Jimenez Borreguero, Jesus] Hosp La Princesa, Dept Cardiol, Madrid, Spain. [Ordovas, Jose M.] Tufts Univ, Human Nutr Res Ctr Aging, USDA, Nutr & Genom Lab, Boston, MA 02111 USA. [Ordovas, Jose M.] Madrid Inst Adv Studies Food IMDEA Food, Madrid, Spain. [Pocovi, Miguel] Univ Zaragoza, Dept Biochem Mol & Cell Biol, Inst Aragonas Ciencias Salud I CS, Zaragoza, Spain. [Pocovi, Miguel] Ctr Biomed Network Res Rare Dis CIBERER, Zaragoza, Spain. [Fuster, Valentin] Mt Sinai Sch Med, Zena & Michael A Wiener Cardiovasc Inst, New York, NY USA. [Fuster, Valentin] Mt Sinai Sch Med, Marie Josee & Henry R Kravis Cardiovasc Hlth Ctr, New York, NY USA. RP Guallar, E (reprint author), Natl Ctr Cardiovasc Res CNIC, Dept Epidemiol Atherothrombosis & Imaging, Madrid, Spain. EM eguallar@jhsph.edu RI Guallar, Eliseo/D-3807-2014; Jimenez-Borreguero, Luis Jesus/L-8107-2014; Laclaustra, Martin/C-6709-2015; Ibanez, Borja/J-6993-2014; Fuster, Valentin/H-4319-2015 OI Guallar, Eliseo/0000-0002-4471-9565; Jimenez-Borreguero, Luis Jesus/0000-0001-5870-237X; Laclaustra, Martin/0000-0003-3963-0846; Ibanez, Borja/0000-0002-5036-254X; Fuster, Valentin/0000-0002-9043-9986 FU Spain's Fondo de Investigaciones Sanitarias [FIS PS09/0665, FIS PS09/01936, FIS CP08/00112, PI10/00021] FX Dr. Pocovi was supported in part by grant FIS PS09/0665 from Spain's Fondo de Investigaciones Sanitarias. Dr. Leon was supported in part by grant FIS PS09/01936 from Spain's Fondo de Investigaciones Sanitarias. Dr. Laclaustra was supported in part by grants FIS CP08/00112 and PI10/00021 from Spain's Fondo de Investigaciones Sanitarias. NR 44 TC 18 Z9 18 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2261 J9 BMC CARDIOVASC DISOR JI BMC Cardiovasc. Disord. PD JUN 19 PY 2012 VL 12 AR 45 DI 10.1186/1471-2261-12-45 PG 11 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA 006HH UT WOS:000308809600001 PM 22712826 ER PT J AU Wasik, JKC Mitchell, CPJ Engstrom, DR Swain, EB Monson, BA Balogh, SJ Jeremiason, JD Branfireun, BA Eggert, SL Kolka, RK Almendinger, JE AF Wasik, Jill K. Coleman Mitchell, Carl P. J. Engstrom, Daniel R. Swain, Edward B. Monson, Bruce A. Balogh, Steven J. Jeremiason, Jeffrey D. Branfireun, Brian A. Eggert, Susan L. Kolka, Randall K. Almendinger, James E. TI Methylmercury Declines in a Boreal Peatland When Experimental Sulfate Deposition Decreases SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID DISSOLVED ORGANIC-MATTER; GREAT-LAKES REGION; ATMOSPHERIC MERCURY DEPOSITION; ATOMIC FLUORESCENCE DETECTION; NORTHEASTERN UNITED-STATES; AQUEOUS-PHASE ETHYLATION; GAS-CHROMATOGRAPHY; ACID DEPOSITION; METHYL MERCURY; MINNESOTA AB Between 2001 and 2008 we experimentally manipulated atmospheric sulfate-loading to a small boreal peatland and monitored the resulting short and long-term changes in methylmercury (MeHg) production. MeHg concentrations and %MeHg (fraction of total-Hg (Hg-T) present as MeHg) in the porewaters of the experimental treatment reached peak values within a week of sulfate addition and then declined as the added sulfate disappeared. MeHg increased cumulatively over time in the solid-phase peat, which acted as a sink for newly produced MeHg. In 2006 a "recovery" treatment was created by discontinuing sulfate addition to a portion of the experimentally treated section to assess how MeHg production might respond to decreased sulfate loads. Four years after sulfate additions ceased, MeHg concentrations and %MeHg had declined significantly from 2006 values in porewaters and peat, but remained elevated relative to control levels. Mosquito larvae collected from each treatment at the end of the experiment exhibited Hg-T concentrations reflective of MeHg levels in the peat and porewaters where they were collected. The proportional responses of invertebrate Hg-T to sulfate deposition rates demonstrate that further controls on sulfur emissions may represent an additional means of mitigating Hg contamination in fish and wildlife across low-sulfur landscapes. C1 [Wasik, Jill K. Coleman] Univ Minnesota, Water Resources Sci Grad Program, St Paul, MN 55108 USA. [Wasik, Jill K. Coleman; Engstrom, Daniel R.; Almendinger, James E.] Sci Museum Minnesota, St Croix Watershed Res Stn, Marine St Croix, MN 55047 USA. [Mitchell, Carl P. J.] Univ Toronto Scarborough, Dept Phys & Environm Sci, Toronto, ON M1C 1A4, Canada. [Swain, Edward B.; Monson, Bruce A.] Minnesota Pollut Control Agcy, Environm Anal & Outcomes, St Paul, MN 55155 USA. [Balogh, Steven J.] Metropolitan Council Environm Serv, St Paul, MN 55106 USA. [Jeremiason, Jeffrey D.] Gustavus Adolphus Coll, Dept Chem, St Peter, MN 56082 USA. [Branfireun, Brian A.] Univ Western Ontario, Dept Biol, London, ON N6A 5B7, Canada. [Eggert, Susan L.; Kolka, Randall K.] US Forest Serv, No Res Stn, USDA, Grand Rapids, MN 55744 USA. RP Wasik, JKC (reprint author), Univ Minnesota, Water Resources Sci Grad Program, 173 McNeal Hall,1985 Buford Ave, St Paul, MN 55108 USA. EM colemajk@umn.edu RI Mitchell, Carl/A-7212-2008 OI Mitchell, Carl/0000-0001-8538-5138 FU U.S. EPA-Science To Achieve Results (STAR) Program [R827630]; Great Lakes Commission, Great Lakes Air Deposition program; Minnesota Pollution Control Agency FX Funding for this long-term project came from the U.S. EPA-Science To Achieve Results (STAR) Program, Grant R827630, the Great Lakes Commission, Great Lakes Air Deposition program, and the Minnesota Pollution Control Agency. The USDA Forest Service's Northern Research Station provided access to the study site as well as substantial in-kind support. Many individuals assisted with sulfate additions and sample collection including P. Hoff and D. Helwig (Minnesota Pollution Control Agency), A. Stephens, A. Baczynski, and W. Daniels (St. Croix Watershed Research Station), and J. Heissel, C. Green, and J. Westlake (Northern Research Station). We also gratefully acknowledge the support of the analysts and technicians including Y. Nollet (Metropolitan Council Environmental Services), A. Hong (Univ. of Toronto-Scarborough), D. Nelson and J. Larson (Northern Research Station), and C. Eckley and M. Collins (Univ. of Toronto-Mississauga). Special thanks go to R. Kyllander and C. Dorrance at the Northern Research Station for administrative and field assistance throughout the course of the project. NR 56 TC 13 Z9 13 U1 3 U2 61 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 19 PY 2012 VL 46 IS 12 BP 6663 EP 6671 DI 10.1021/es300865f PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 959OA UT WOS:000305320900030 ER PT J AU Hong, T Gurian, PL AF Hong, Tao Gurian, Patrick L. TI Characterizing Bioaerosol Risk from Environmental Sampling SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID BACILLUS-ANTHRACIS SPORES; DOSE-RESPONSE MODELS; INHALATIONAL ANTHRAX; CONTAMINANT RELEASES; BIOTERRORISM; BUILDINGS; AEROSOLIZATION; FRAMEWORK; DYNAMICS; OUTBREAK AB In the aftermath of a release of microbiological agents, environmental sampling must be conducted to characterize the release sufficiently so that mathematical models can then be used to predict the subsequent dispersion and human health risks. Because both the dose-response and environmental transport of aerosolized microbiological agents are functions of the effective aerodynamic diameter of the particles, environmental assessments should consider not only the total amount of agents but also the size distributions of the aerosolized particles. However, typical surface sampling cannot readily distinguish among different size particles. This study evaluates different approaches to estimating risk from measurements of microorganisms deposited on surfaces after an aerosol release. For various combinations of sampling surfaces, size fractions, HVAC operating conditions, size distributions of release spores, uncertainties in surface measurements, and the accuracy of model predictions are tested in order to assess how much detail can realistically be identified from surface sampling results. The recommended modeling and sampling scheme is one choosing 3, 5, and 10 mu m diameter particles as identification targets and taking samples from untracked floor, wall, and the HVAC filter. This scheme provides reasonably accurate, but somewhat conservative, estimates of risk across a range of different scenarios. Performance of the recommended sampling scheme is tested by using data from a large-scale field test as a case study. Sample sizes of 10-25 in each homogeneously mixed environmental compartment are sufficient to develop order of magnitude estimates of risk. Larger sample sizes have little benefit unless uncertainties in sample recoveries can be reduced. C1 [Hong, Tao; Gurian, Patrick L.] Drexel Univ, Dept Civil Architectural & Environm Engn, Philadelphia, PA 19104 USA. RP Hong, T (reprint author), USDA ARS, Off Res & Dev, 960 Coll Stn Rd, Athens, GA 30605 USA. EM hongtao510@gmail.com RI Gurian, Patrick/A-8365-2013 OI Gurian, Patrick/0000-0001-7456-9740 FU U.S. Environmental Protection Agency; U.S. Department of Homeland Security, under the Science to Achieve Results (STAR) grant program [R83236201]; Center for Advancing Microbial Risk Assessment FX This research was funded through the Center for Advancing Microbial Risk Assessment, supported by the U.S. Environmental Protection Agency and U.S. Department of Homeland Security, under the Science to Achieve Results (STAR) grant program (grant R83236201). We appreciate valuable discussion and inputs from Ken Fan and Nicholas Dudley Ward. NR 43 TC 5 Z9 5 U1 1 U2 26 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 19 PY 2012 VL 46 IS 12 BP 6714 EP 6722 DI 10.1021/es300197n PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 959OA UT WOS:000305320900036 PM 22568610 ER PT J AU Mitchell, GB Sigurdson, CJ O'Rourke, KI Algire, J Harrington, NP Walther, I Spraker, TR Balachandran, A AF Mitchell, Gordon B. Sigurdson, Christina J. O'Rourke, Katherine I. Algire, James Harrington, Noel P. Walther, Ines Spraker, Terry R. Balachandran, Aru TI Experimental Oral Transmission of Chronic Wasting Disease to Reindeer (Rangifer tarandus tarandus) SO PLOS ONE LA English DT Article ID DEER ODOCOILEUS-HEMIONUS; WHITE-TAILED DEER; RESISTANT PRION PROTEIN; CERVUS-ELAPHUS-NELSONI; CAPTIVE MULE DEER; SPONGIFORM ENCEPHALOPATHY; STRAIN MUTATION; TRANSGENIC MICE; FALLOW DEER; ELK AB Chronic wasting disease (CWD), a transmissible spongiform encephalopathy of cervids, remains prevalent in North American elk, white-tailed deer and mule deer. A natural case of CWD in reindeer (Rangifer tarandus tarandus) has not been reported despite potential habitat overlap with CWD-infected deer or elk herds. This study investigates the experimental transmission of CWD from elk or white-tailed deer to reindeer by the oral route of inoculation. Ante-mortem testing of the three reindeer exposed to CWD from white-tailed deer identified the accumulation of pathological PrP (PrPCWD) in the recto-anal mucosa associated lymphoid tissue (RAMALT) of two reindeer at 13.4 months post-inoculation. Terminal CWD occurred in the two RAMALT-positive reindeer at 18.5 and 20 months post-inoculation while one other reindeer in the white-tailed deer CWD inoculum group and none of the 3 reindeer exposed to elk CWD developed disease. Tissue distribution analysis of PrPCWD in CWD-affected reindeer revealed widespread deposition in central and peripheral nervous systems, lymphoreticular tissues, the gastrointestinal tract, neuroendocrine tissues and cardiac muscle. Analysis of prion protein gene (PRNP) sequences in the 6 reindeer identified polymorphisms at residues 2 (V/M), 129 (G/S), 138 (S/N) and 169 (V/M). These findings demonstrate that (i) a sub-population of reindeer are susceptible to CWD by oral inoculation implicating the potential for transmission to other Rangifer species, and (ii) certain reindeer PRNP polymorphisms may be protective against CWD infection. C1 [Mitchell, Gordon B.; Algire, James; Harrington, Noel P.; Walther, Ines; Balachandran, Aru] Canadian Food Inspect Agcy, Ottawa Lab Fallowfield, Natl & OIE Reference Lab Scrapie & CWD, Ottawa, ON, Canada. [Sigurdson, Christina J.] Univ Calif San Diego, Dept Pathol, La Jolla, CA 92093 USA. [Sigurdson, Christina J.] Univ Calif San Diego, Dept Med, La Jolla, CA 92093 USA. [Sigurdson, Christina J.] Univ Calif Davis, Dept Pathol Microbiol & Immunol, Davis, CA 95616 USA. [O'Rourke, Katherine I.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [Spraker, Terry R.] Colorado State Univ, Vet Diagnost Lab, Ft Collins, CO 80523 USA. RP Mitchell, GB (reprint author), Canadian Food Inspect Agcy, Ottawa Lab Fallowfield, Natl & OIE Reference Lab Scrapie & CWD, Ottawa, ON, Canada. EM Aru.Balachandran@inspection.gc.ca FU Canadian Food Inspection Agency; Agriculture and Agri-Food Canada [C00233]; United States Department of Agriculture Agricultural Research Service [CRIS 5348-32000-026-00-D]; United States National Institutes of Health [NS069566, U54AI0359] FX This work was supported by the Canadian Food Inspection Agency and Agriculture and Agri-Food Canada under C00233, the United States Department of Agriculture Agricultural Research Service under CRIS 5348-32000-026-00-D and the United States National Institutes of Health under NS069566 and U54AI0359. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 14 Z9 14 U1 3 U2 22 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 18 PY 2012 VL 7 IS 6 AR e39055 DI 10.1371/journal.pone.0039055 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 962XO UT WOS:000305583300086 PM 22723928 ER PT J AU Zehr, ES Lavrov, DV Tabatabai, LB AF Zehr, Emilie S. Lavrov, Dennis V. Tabatabai, Louisa B. TI Comparison of Haemophilus parasuis reference strains and field isolates by using random amplified polymorphic DNA and protein profiles SO BMC MICROBIOLOGY LA English DT Article DE Haemophilus parasuis; RAPD; SDS-PAGE ID MULTILOCUS ENZYME ELECTROPHORESIS; ERIC-PCR; SEROLOGICAL CHARACTERIZATION; INDIRECT HEMAGGLUTINATION; MOLECULAR EPIDEMIOLOGY; KLEBSIELLA-PNEUMONIAE; RANDOM AMPLIFICATION; ARBITRARY PRIMERS; GLASSERS-DISEASE; GENOME SEQUENCE AB Background: Haemophilus parasuis is the causative agent of Glasser's disease and is a pathogen of swine in high-health status herds. Reports on serotyping of field strains from outbreaks describe that approximately 30% of them are nontypeable and therefore cannot be traced. Molecular typing methods have been used as alternatives to serotyping. This study was done to compare random amplified polymorphic DNA (RAPD) profiles and whole cell protein (WCP) lysate profiles as methods for distinguishing H. parasuis reference strains and field isolates. Results: The DNA and WCP lysate profiles of 15 reference strains and 31 field isolates of H. parasuis were analyzed using the Dice and neighbor joining algorithms. The results revealed unique and reproducible DNA and protein profiles among the reference strains and field isolates studied. Simpson's index of diversity showed significant discrimination between isolates when three 10mer primers were combined for the RAPD method and also when both the RAPD and WCP lysate typing methods were combined. Conclusions: The RAPD profiles seen among the reference strains and field isolates did not appear to change over time which may reflect a lack of DNA mutations in the genes of the samples. The recent field isolates had different WCP lysate profiles than the reference strains, possibly because the number of passages of the type strains may affect their protein expression. C1 [Zehr, Emilie S.; Tabatabai, Louisa B.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Lavrov, Dennis V.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50010 USA. RP Zehr, ES (reprint author), ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM emilie.zehr@ars.usda.gov OI Lavrov, Dennis/0000-0002-2745-1704 NR 64 TC 5 Z9 6 U1 1 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD JUN 15 PY 2012 VL 12 AR 108 DI 10.1186/1471-2180-12-108 PG 14 WC Microbiology SC Microbiology GA 038EN UT WOS:000311158100001 PM 22703293 ER PT J AU Park, W Scheffler, BE Bauer, PJ Campbell, BT AF Park, Wonkeun Scheffler, Brian E. Bauer, Philip J. Campbell, B. Todd TI Genome-wide identification of differentially expressed genes under water deficit stress in upland cotton (Gossypium hirsutum L.) SO BMC PLANT BIOLOGY LA English DT Article ID TOBACCO AQUAPORIN NTAQP1; HIGH-SALINITY STRESSES; CDNA-AFLP ANALYSIS; DROUGHT-STRESS; ABSCISIC-ACID; PHYSIOLOGICAL-RESPONSES; SIGNAL-TRANSDUCTION; EVOLUTIONARY NOVELTY; ARABIDOPSIS-THALIANA; ABIOTIC STRESSES AB Background: Cotton is the world's primary fiber crop and is a major agricultural commodity in over 30 countries. Like many other global commodities, sustainable cotton production is challenged by restricted natural resources. In response to the anticipated increase of agricultural water demand, a major research direction involves developing crops that use less water or that use water more efficiently. In this study, our objective was to identify differentially expressed genes in response to water deficit stress in cotton. A global expression analysis using cDNA-Amplified Fragment Length Polymorphism was conducted to compare root and leaf gene expression profiles from a putative drought resistant cotton cultivar grown under water deficit stressed and well watered field conditions. Results: We identified a total of 519 differentially expressed transcript derived fragments. Of these, 147 transcript derived fragment sequences were functionally annotated according to their gene ontology. Nearly 70 percent of transcript derived fragments belonged to four major categories: 1) unclassified, 2) stress/defense, 3) metabolism, and 4) gene regulation. We found heat shock protein-related and reactive oxygen species-related transcript derived fragments to be among the major parts of functional pathways induced by water deficit stress. Also, twelve novel transcripts were identified as both water deficit responsive and cotton specific. A subset of differentially expressed transcript derived fragments was verified using reverse transcription-polymerase chain reaction. Differential expression analysis also identified five pairs of duplicated transcript derived fragments in which four pairs responded differentially between each of their two homologues under water deficit stress. Conclusions: In this study, we detected differentially expressed transcript derived fragments from water deficit stressed root and leaf tissues in tetraploid cotton and provided their gene ontology, functional/biological distribution, and possible roles of gene duplication. This discovery demonstrates complex mechanisms involved with polyploid cotton's transcriptome response to naturally occurring field water deficit stress. The genes identified in this study will provide candidate targets to manipulate the water use characteristics of cotton at the molecular level. C1 [Park, Wonkeun; Bauer, Philip J.; Campbell, B. Todd] USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. [Scheffler, Brian E.] USDA ARS, MSA Genom Lab, Stoneville, MS 38776 USA. RP Campbell, BT (reprint author), USDA ARS, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. EM todd.campbell@ars.usda.gov OI Scheffler, Brian/0000-0003-1968-8952 FU CRIS of the U.S. Department of Agriculture [6657-21000-006-00D]; Cotton Incorporated FX This research project was supported by funding from CRIS No. 6657-21000-006-00D of the U.S. Department of Agriculture and a grant from Cotton Incorporated. Authors acknowledge assistance from Dr. Paxton Payton for the RNA isolation protocol, Drs. Joshua Udall and Martin Wubben for critically reviewing this manuscript, Drs. Michel Claverie and Jean Marc Lacape for the cDNA-AFLP protocol, Fanny Liu for DNA sequencing, and Dr. Greg Constable for supplying cotton seeds. We acknowledge Mr. Jeff Chandler and North Carolina State University Sandhills Research Station staff for providing excellent technical field plot support. We also thank Ernie Strickland, Kendreal Wingate, and Bobby Fisher for their technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 86 TC 20 Z9 22 U1 0 U2 47 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUN 15 PY 2012 VL 12 AR 90 DI 10.1186/1471-2229-12-90 PG 12 WC Plant Sciences SC Plant Sciences GA 004PP UT WOS:000308693900001 PM 22703539 ER PT J AU Sui, Y Liu, J Wisniewski, M Droby, S Norelli, J Hershkovitz, V AF Sui, Yuan Liu, Jia Wisniewski, Michael Droby, Samir Norelli, John Hershkovitz, Vera TI Pretreatment of the yeast antagonist, Candida oleophila, with glycine betaine increases oxidative stress tolerance in the microenvironment of apple wounds SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Antioxidant genes; Biocontrol yeast; Glycine betaine; Reactive oxygen species ID POSTHARVEST FRUIT DISEASES; PANTOEA-AGGLOMERANS CPA-2; BIOLOGICAL-CONTROL; BIOCONTROL AGENT; METSCHNIKOWIA-FRUCTICOLA; CRYPTOCOCCUS-LAURENTII; MICROBIAL ANTAGONISTS; PENICILLIUM-DIGITATUM; GENE-EXPRESSION; OSMOTIC-STRESS AB In response to wounding, harvested fruit tissues of apple and citrus exhibit the production of reactive oxygen species (ROS). ROS production is greater when yeast antagonists used as biocontrol agents are applied in the wounds. These phenomena result in an oxidative stress environment for the yeast antagonists. It has been demonstrated that pre-exposure of some of these yeast antagonists to sublethal abiotic stress (heat or hydrogen peroxide), or stress-ameliorating compounds such as glycine betaine (GB) can induce subsequent oxidative stress tolerance in the antagonistic yeast. The increased level of oxidative stress tolerance has been demonstrated in vitro and is characterized by higher levels of antioxidant gene expression, increased production of trehalose, and lower levels of ROS when yeast are exposed to a subsequent oxidative stress. The current study determined whether or not the effects of GB on yeast antagonists determined in vitro persist and are present in planta when yeast are applied to wounded apples. The effect of exogenous GB on the production of ROS in the yeast antagonist, Candida oleophila, was determined after the yeast was placed in apple wounds. Oxidative damage to yeast cells recovered from apple wounds was also monitored. Results indicated that GB treatment improved the adaptation of C. oleophila to apple fruit wounds. Compared to untreated control yeast cells, GB-treated cells recovered from the oxidative stress environment of apple wounds exhibited less accumulation of ROS and lower levels of oxidative damage to cellular proteins and lipids. Additionally, GB-treated yeast exhibited greater biocontrol activity against Penicillium expansum and Botrytis cinerea, and faster growth in wounds of apple fruits compared to untreated yeast. The expression of major antioxidant genes, including peroxisomal catalase, peroxiredoxin TSA1, and glutathione peroxidase was elevated in the yeast by GB treatment. This study supports the premise that activation of antioxidant response in biocontrol yeast can improve biocontrol efficacy. Published by Elsevier B.V. C1 [Sui, Yuan; Liu, Jia; Wisniewski, Michael; Norelli, John] ARS, USDA, Kearneysville, WV 25430 USA. [Droby, Samir; Hershkovitz, Vera] Agr Res Org, Volcani Ctr, IL-50250 Bet Dagan, Israel. RP Wisniewski, M (reprint author), ARS, USDA, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.wisniewski@ars.usda.gov FU U.S.-Israel Binational Agricultural Research and Development (BARD) Fund [IS-4268-09] FX This research was partially supported by a grant (IS-4268-09) from the U.S.-Israel Binational Agricultural Research and Development (BARD) Fund to S. D. and M. W. NR 62 TC 9 Z9 10 U1 1 U2 48 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JUN 15 PY 2012 VL 157 IS 1 BP 45 EP 51 DI 10.1016/j.ijfoodmicro.2012.04.010 PG 7 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA 966WV UT WOS:000305869000006 PM 22560021 ER PT J AU Gobster, PH Xiang, WN AF Gobster, Paul H. Xiang, Wei-Ning TI What do we mean by "landscape"? SO LANDSCAPE AND URBAN PLANNING LA English DT Editorial Material C1 [Gobster, Paul H.] US Forest Serv, USDA, No Res Stn 1033, Evanston, IL 60201 USA. [Xiang, Wei-Ning] E China Normal Univ, Shanghai Inst Urban Ecol & Sustainabil, Shanghai Key Lab Urban Ecol Proc & Ecorestorat, Shanghai 200062, Peoples R China. RP Gobster, PH (reprint author), US Forest Serv, USDA, No Res Stn 1033, Univ Pl,Suite 360, Evanston, IL 60201 USA. EM pgobster@gmail.com; wnxiang@mail.ecnu.edu.cn RI Gobster, Paul/A-2826-2013 OI Gobster, Paul/0000-0002-8576-0310 NR 8 TC 1 Z9 1 U1 2 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD JUN 15 PY 2012 VL 106 IS 3 BP 219 EP 220 DI 10.1016/j.landurbplan.2012.05.004 PG 2 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 960GN UT WOS:000305376100001 ER PT J AU Troy, A Grove, JM O'Neil-Dunne, J AF Troy, Austin Grove, J. Morgan O'Neil-Dunne, Jarlath TI The relationship between tree canopy and crime rates across an urban-rural gradient in the greater Baltimore region SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Urban tree canopy; Crime; Urban vegetation; Public safety; Geographically weighted regression ID GEOGRAPHICALLY WEIGHTED REGRESSION; SHADE TREES; AIR-QUALITY; GREEN AREAS; INNER-CITY; ENERGY USE; VEGETATION; ECOSYSTEMS; COMMUNITY; PATTERNS AB The extent to which urban tree cover influences crime is in debate in the literature. This research took advantage of geocoded crime point data and high resolution tree canopy data to address this question in Baltimore City and County. MD, an area that includes a significant urban-rural gradient. Using ordinary least squares and spatially adjusted regression and controlling for numerous potential confounders, we found that there is a strong inverse relationship between tree canopy and our index of robbery, burglary, theft and shooting. The more conservative spatially adjusted model indicated that a 10% increase in tree canopy was associated with a roughly 12% decrease in crime. When we broke down tree cover by public and private ownership for the spatial model, we found that the inverse relationship continued in both contexts, but the magnitude was 40% greater for public than for private lands. We also used geographically weighted regression to identify spatial non-stationarity in this relationship, which we found for trees in general and trees on private land, but not for trees on public land. Geographic plots of pseudo-t statistics indicated that while there was a negative relationship between crime and trees in the vast majority of block groups of the study area, there were a few patches where the opposite relationship was true, particularly in a part of Baltimore City where there is an extensive interface between industrial and residential properties. It is possible that in this area a significant proportion of trees is growing in abandoned lands between these two land uses. (C) 2012 Elsevier B.V. All rights reserved. C1 [Troy, Austin; O'Neil-Dunne, Jarlath] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Aiken Ctr, Burlington, VT 05405 USA. [Troy, Austin; O'Neil-Dunne, Jarlath] Univ Vermont, Spatial Anal Lab, Aiken Ctr, Burlington, VT 05405 USA. [Grove, J. Morgan] US Forest Serv, USDA, No Res Stn, Washington, DC USA. RP Troy, A (reprint author), Univ Vermont, Rubenstein Sch Environm & Nat Resources, Aiken Ctr, 81 Carrigan Dr, Burlington, VT 05405 USA. EM atroy@uvm.edu; mgrove@fs.fed.us; Jarlath.ONeil-Dunne@uvm.edu FU U.S. Forest Service's Northern Research Station and Northeastern Area State & Private Forestry Program [USDA03-CA-11244225-531]; National Science Foundation Long-Term Ecological Research program [NSF DEB-0423476]; National Science Foundation Human and Social Dynamics Program [0624159] FX This research was made possible by the generous support of the U.S. Forest Service's Northern Research Station and Northeastern Area State & Private Forestry Program (USDA03-CA-11244225-531), the National Science Foundation Long-Term Ecological Research program (NSF DEB-0423476) and the National Science Foundation Human and Social Dynamics Program (award #0624159), which in turn supported the Baltimore Ecosystem Study. Thanks also go out to R. Holli Howard for her expert GIS help and to Colin Drane of Spotcrime for his help with interpreting crime data. NR 39 TC 41 Z9 46 U1 4 U2 64 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD JUN 15 PY 2012 VL 106 IS 3 BP 262 EP 270 DI 10.1016/j.landurbplan.2012.03.010 PG 9 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 960GN UT WOS:000305376100006 ER PT J AU Shriner, SA VanDalen, KK Mooers, NL Ellis, JW Sullivan, HJ Root, JJ Pelzel, AM Franklin, AB AF Shriner, Susan A. VanDalen, Kaci K. Mooers, Nicole L. Ellis, Jeremy W. Sullivan, Heather J. Root, J. Jeffrey Pelzel, Angela M. Franklin, Alan B. TI Low-Pathogenic Avian Influenza Viruses in Wild House Mice SO PLOS ONE LA English DT Article ID A H5N1 VIRUS; HONG-KONG; MUS-MUSCULUS; MOUSE MODEL; MX1 GENE; INFECTION; POULTRY; FERRETS; TRANSMISSION; OUTBREAK AB Background: Avian influenza viruses are known to productively infect a number of mammal species, several of which are commonly found on or near poultry and gamebird farms. While control of rodent species is often used to limit avian influenza virus transmission within and among outbreak sites, few studies have investigated the potential role of these species in outbreak dynamics. Methodology/Principal Findings: We trapped and sampled synanthropic mammals on a gamebird farm in Idaho, USA that had recently experienced a low pathogenic avian influenza outbreak. Six of six house mice (Mus musculus) caught on the outbreak farm were presumptively positive for antibodies to type A influenza. Consequently, we experimentally infected groups of naive wild-caught house mice with five different low pathogenic avian influenza viruses that included three viruses derived from wild birds and two viruses derived from chickens. Virus replication was efficient in house mice inoculated with viruses derived from wild birds and more moderate for chicken-derived viruses. Mean titers (EID50 equivalents/mL) across all lung samples from seven days of sampling (three mice/day) ranged from 10(3.89) (H3N6) to 10(5.06) (H4N6) for the wild bird viruses and 10(2.08) (H6N2) to 10(2.85) (H4N8) for the chicken-derived viruses. Interestingly, multiple regression models indicated differential replication between sexes, with significantly (p<0.05) higher concentrations of avian influenza RNA found in females compared with males. Conclusions/Significance: Avian influenza viruses replicated efficiently in wild-caught house mice without adaptation, indicating mice may be a risk pathway for movement of avian influenza viruses on poultry and gamebird farms. Differential virus replication between males and females warrants further investigation to determine the generality of this result in avian influenza disease dynamics. C1 [Shriner, Susan A.; VanDalen, Kaci K.; Mooers, Nicole L.; Ellis, Jeremy W.; Sullivan, Heather J.; Root, J. Jeffrey; Pelzel, Angela M.; Franklin, Alan B.] USDA Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. RP Shriner, SA (reprint author), USDA Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. EM Susan.A.Shriner@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182; Ellis, Jeremy/0000-0003-4512-0906 FU United States Department of Agriculture FX This study was funded by the United States Department of Agriculture. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 68 TC 14 Z9 14 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 15 PY 2012 VL 7 IS 6 AR e39206 DI 10.1371/journal.pone.0039206 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WX UT WOS:000305350000050 PM 22720076 ER PT J AU Dombrowski, JE Martin, RC AF Dombrowski, James E. Martin, Ruth C. TI Abiotic stresses activate a MAPkinase in the model grass species Lolium temulentum SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Grass; Heat; Lolium temulentum; MAPK; Salt; Wounding ID PROTEIN-KINASE GENE; DISEASE RESISTANCE; ENVIRONMENTAL CUES; RICE OSSIPK; PLANTS; TOLERANCE; RESPONSES; COLD; ARABIDOPSIS; EXPRESSION AB Forage and turf grasses are utilized in diverse environments that expose them to a variety of abiotic stresses, however very little is known concerning the perception or molecular responses to these various stresses. In the model grass species Lolium temulentum, a 46 kDa mitogen-activated protein kinase (MAPK) was activated in the leaf within 10 min of exposing the roots to salt stress. When plants were subjected cold stress, no significant activation of the MAPK was observed. However, the 46 kDa MAPK was rapidly activated in the leaves of plants within 3 min of exposure to heat stress. Previously, mechanical wounding has been shown to rapidly activate a 46 kDa and a 44 kDa MAPK in L. temulentum. The wound activation of the MAPKs was delayed and diminished in plants undergoing cold treatment. In plants subjected simultaneously to 40 degrees C and wounding, the activation of the 46 kDa MAPK was enhanced. However if plants were subjected to heat and cold stress for more than 2 h or exposed to 300 mM NaCl for 24 h prior to wounding, the wound activation of the 46 kDa and a 44 kDa MAPKs were significantly inhibited. These results suggest that the 46 kDa MAPK plays a role in the response to various environmental stimuli. Published by Elsevier GmbH. C1 [Dombrowski, James E.; Martin, Ruth C.] USDA ARS, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. RP Dombrowski, JE (reprint author), USDA ARS, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM Jim.Dombrowski@ars.usda.gov; dombrowj@onid.orst.edu NR 33 TC 3 Z9 3 U1 0 U2 8 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD JUN 15 PY 2012 VL 169 IS 9 BP 915 EP 919 DI 10.1016/j.jplph.2012.03.003 PG 5 WC Plant Sciences SC Plant Sciences GA 960AM UT WOS:000305360400010 PM 22472075 ER PT J AU Lizotte, RE Shields, FD Murdock, JN Kroger, R Knight, SS AF Lizotte, Richard E., Jr. Shields, F. Douglas, Jr. Murdock, Justin N. Kroeger, Robert Knight, Scott S. TI Mitigating agrichemicals from an artificial runoff event using a managed riverine wetland SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Floodplain backwater; Hydrologic management; Sediment; Nutrients; Pesticides ID RECEIVING AGRICULTURAL RUNOFF; CONSTRUCTED WETLANDS; MISSISSIPPI DELTA; NITROGEN-RETENTION; PESTICIDE REMOVAL; NUTRIENT-REMOVAL; WATER; PHOSPHORUS; SEDIMENT; USA AB We examined the mitigation efficiency of a managed riverine wetland amended with a mixture of suspended sediment, two nutrients (nitrogen and phosphorus), and three pesticides (atrazine, metolachlor, and permethrin) during a simulated agricultural runoff event. Hydrologic management of the 500 m-long, 25 m-wide riverine wetland was done by adding weirs at both ends. The agrichemical mixture was amended to the wetland at the upstream weir simulating a four-hour, similar to 1 cm rainfall event from a 16 ha agricultural field. Water samples (1 L) were collected every 30 min within the first 4 h, then every 4 h until 48 h, and again on days 5, 7, 14, 21, and 28 post-amendment at distances of 0 m, 10 m, 40 m, 300 in and 500 m from the amendment point within the wetland for suspended solids, nutrient, and pesticide analyses. Peak sediment, nutrient, and pesticide concentrations occurred within 3 h of amendment at 0 m, 10 m, 40 m, and 300 m downstream and showed rapid attenuation of agrichemicals from the water column with 79-98%, 42-98%. and 63-98% decrease in concentrations of sediments. nutrients, and pesticides, respectively, within 48 h. By day 28, all amendments were near or below pre-amendment concentrations. Water samples at 500 m showed no changes in sediment or nutrient concentrations: pesticide concentrations peaked within 48 h but at <= 11% of upstream peak concentrations and had dissipated by day 28. Managed riverine wetlands >= 1 ha and with hydraulic residence times of days to weeks can efficiently trap agricultural runoff during moderate (1 cm) late-spring and early-summer rainfall events, mitigating impacts to receiving rivers. Published by Elsevier B.V. C1 [Lizotte, Richard E., Jr.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. Mississippi State Univ, Dept Wildlife Fisheries & Aquaculture, Mississippi State, MS 39762 USA. RP Lizotte, RE (reprint author), USDA ARS, Natl Sedimentat Lab, POB 1157, Oxford, MS 38655 USA. EM Richard.lizotte@ars.usda.gov NR 50 TC 8 Z9 8 U1 3 U2 26 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUN 15 PY 2012 VL 427 BP 373 EP 381 DI 10.1016/j.scitotenv.2012.04.025 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA 956TF UT WOS:000305111300042 PM 22560749 ER PT J AU Raybuck, AL Moorman, CE Greenberg, CH DePerno, CS Gross, K Simon, DM Warburton, GS AF Raybuck, Amy L. Moorman, Christopher E. Greenberg, Cathryn H. DePerno, Christopher S. Gross, Kevin Simon, Dean M. Warburton, Gordon S. TI Short-term response of small mammals following oak regeneration silviculture treatments SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Herbicide; Mice; Prescribed fire; Shelterwood harvest; Shrew; Southern Appalachians ID SOUTHERN APPALACHIAN MOUNTAINS; MECHANICAL FUEL REDUCTION; UPLAND HARDWOOD FOREST; COARSE WOODY DEBRIS; WHITE-FOOTED MOUSE; OLD-GROWTH FOREST; PEROMYSCUS-LEUCOPUS; PINE FOREST; PRESCRIBED FIRE; POPULATIONS AB Upland, mixed-oak forests in the eastern United States have experienced widespread oak regeneration failure, largely due to cessation of anthropogenic disturbance. Silvicultural practices used to promote advance oak regeneration may affect ground-dwelling mammals. From May to August 2008 (pre-treatment), 2010 (first year post-treatment), and 2011 (second year post-treatment), we trapped small mammals to assess changes in species richness and abundance following experimental tests of three silvicultural treatments (prescribed burns, midstory herbicide applications, and shelterwood harvests) used to promote oak regeneration. We trapped small mammals in five replicates of each treatment and controls using Sherman live traps (2008 and 2010) and drift fences (2008, 2010, and 2011). From pre- to post-treatment, we evaluated the change in estimated peromyscid abundance and relative abundance of masked shrews (Sorex cinereus), smoky shrews (Sorex fumeus), and northern short-tailed shrews (Blarina brevicauda). Additionally, we evaluated the change in species richness across treatments for both sampling techniques. For all measures analyzed (i.e., species richness, peromyscid abundance, and relative abundance of shrews), the change from pre- to post-treatment did not differ among treatments. However, more masked shrews, smoky shrews, and northern short-tailed shrews were captured in 2011 (i.e., second year post-treatment) than in 2010 (i.e., first year post-treatment). Our research indicates that, in the short-term, small mammals (e.g., mice and shrews) can tolerate a wide range of forest disturbance following oak regeneration treatments. However, delayed treatment effects (e.g., additional post-herbicide midstory dieback) or additive changes following future treatments (e.g., prescribed burns following shelterwood harvests or multiple prescribed burns) may compound effects on small mammal populations, and should be assessed with long-term research (> 2 years post-treatment). (C) 2012 Elsevier B.V. All rights reserved. C1 [Raybuck, Amy L.; Moorman, Christopher E.; DePerno, Christopher S.] N Carolina State Univ, Dept Forestry & Environm Resources, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. [Greenberg, Cathryn H.] US Forest Serv, USDA, So Res Stn, Asheville, NC 28806 USA. [Gross, Kevin] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. [Simon, Dean M.] N Carolina Wildlife Resources Commiss, Lawndale, NC 28090 USA. [Warburton, Gordon S.] N Carolina Wildlife Resources Commiss, Marion, NC 28752 USA. RP Raybuck, AL (reprint author), N Carolina State Univ, Dept Forestry & Environm Resources, Fisheries Wildlife & Conservat Biol Program, Box 7646, Raleigh, NC 27695 USA. EM amy_raybuck@live.com; chris_moorman@ncsu.edu; kgreenberg@fs.fed.us; chris_deperno@ncsu.edu; kevin_gross@ncsu.edu; dean.simon@ncwildlife.org; gordon.warburton@ncwildlife.org FU USDA Forest Service Southern Research Station (SRS); North Carolina State University Fisheries, Wildlife, and Conservation Biology FX This is a contribution of the Regional Oak Study (ROS). Funding was provided by the USDA Forest Service Southern Research Station (SRS) and North Carolina State University Fisheries, Wildlife, and Conservation Biology Program. This research was initiated by the USDA Forest Service, Southern Research Station, Upland Hardwood Ecology and Management Research Work Unit (RWU 4157) in partnership with the USFS Northern Research Station, the North Carolina Wildlife Resources Commission (NCWRC), the Stevenson Land Company, and the Mark Twain National Forest. We thank the many individuals who assisted: K. Frick, T. Wait, A. Paoletta, R. Medford, M. Williams, C. Matthews, A. Tschirley, S. Smith, K. Dziwulski, J. Fields, D. Moffitt, J. Golden, S. Ogburn, J. Trammel, J. Sullivan, P. Helm, E. Greene, A. Warner, M.B. Howard, F. Raybuck, and others who volunteered their time. We thank T. Roof, J. Adams, and site manager T.L. Keyser for providing logistical support, and many other individuals from NCWRC who assisted in the project. NR 62 TC 6 Z9 7 U1 5 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2012 VL 274 BP 10 EP 16 DI 10.1016/j.foreco.2012.02.012 PG 7 WC Forestry SC Forestry GA 948PQ UT WOS:000304515500002 ER PT J AU Safford, HD Stevens, JT Merriam, K Meyer, MD Latimer, AM AF Safford, H. D. Stevens, J. T. Merriam, K. Meyer, M. D. Latimer, A. M. TI Fuel treatment effectiveness in California yellow pine and mixed conifer forests SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fuel treatment; Fire severity; Yellow pine forests; Mixed conifer forests; Sierra Nevada; California ID WESTERN UNITED-STATES; FIRE SEVERITY; SIERRA-NEVADA; VEGETATION STRUCTURE; CLIMATE-CHANGE; WILDFIRE; AREA; US AB We assessed the effectiveness of forest fuel thinning projects that explicitly removed surface and ladder fuels (all but one were combined mechanical and prescribed fire/pile burn prescriptions) in reducing fire severity and tree mortality in 12 forest fires that burned in eastern and southern California between 2005 and 2011. All treatments and fires occurred in yellow pine or mixed conifer forests, in a variety of landscape conditions. Most fires burned under warm, dry conditions, with moderate to high winds. With few exceptions, fire severity measures (bole char height, scorch and torch height, scorch and torch percentage) and tree mortality were much lower in forest stands treated for fuels than in neighboring untreated stands. Fire-tolerant species like Pinus jeffreyi and Pinus ponderosa exhibited much higher postfire survivorship than fire-intolerant species like Abies concolor. Among variables related to fire weather, fuel loading, and treatment age, ten-hour fuel moisture was found to be a better predictor of tree survival in untreated stands than in treated stands, while fuel loading was a better predictor of survival in treated stands. We did not find an effect of treatment age, but our oldest treatments (nine years when burned) were below the mean pre-Euroamerican settlement fire return interval for these forest types. Within treatments, fire severity decreased with distance from the treatment boundary, and canopy fires were almost always reduced to surface fires within 70 m of entering the treatment. In California yellow pine and mixed conifer forests, treatment prescriptions should allow for levels of fire-driven canopy tree mortality (c. 5-15%) that better mimic natural fires. Our results add significantly to the growing evidence that fuel treatments that include removal of surface and ladder fuels in these forest types are highly effective management tools for reducing fire severity and canopy tree mortality. In our opinion, quantitative assessments of fuel treatment effects on fire severity in frequent-fire forest types hardly merit further effort. Rather, we suggest that future work focus on documenting and comparing other ecological outcomes of fuel treatments in burned and unburned forest, such as effects on plant and animal diversity, soil conditions, and habitat heterogeneity. Published by Elsevier B.V. C1 [Safford, H. D.] US Forest Serv, USDA, Vallejo, CA 94592 USA. [Safford, H. D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Stevens, J. T.; Latimer, A. M.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Merriam, K.] US Forest Serv, USDA, Quincy, CA 95971 USA. [Meyer, M. D.] US Forest Serv, USDA, Clovis, CA 93611 USA. RP Safford, HD (reprint author), US Forest Serv, USDA, Vallejo, CA 94592 USA. EM hughsafford@fs.fed.us OI Stevens, Jens/0000-0002-2234-1960 FU USDA Forest Service Pacific Southwest Region; California Energy Commission FX We would like to thank the following individuals for their invaluable help in the field and laboratory: Dave Schmidt, Amy Jirka, Taylor Farnum, Angelica Sauceda, Sveta Yegorova, Susan Magnoli, and Karina Silvas-Bellanca. Larry Hood kindly provided ERC, fuel moisture and weather data for the dates our sampled treatments burned. Thanks also to our editor and two anonymous reviewers, whose comments greatly improved our manuscript. Our work was funded by the USDA Forest Service Pacific Southwest Region, and the California Energy Commission. NR 62 TC 44 Z9 44 U1 3 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2012 VL 274 BP 17 EP 28 DI 10.1016/j.foreco.2012.02.013 PG 12 WC Forestry SC Forestry GA 948PQ UT WOS:000304515500003 ER PT J AU Samuelson, LJ Stokes, TA Johnsen, KH AF Samuelson, Lisa J. Stokes, Tom A. Johnsen, Kurt H. TI Ecophysiological comparison of 50-year-old longleaf pine, slash pine and loblolly pine SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Pinus palustris; Pinus elliottii; Pinus taeda; Water use efficiency; Photosynthesis; Leaf water potential ID TEMPERATURE RESPONSE; TAEDA L.; STOMATAL CONDUCTANCE; NET PHOTOSYNTHESIS; CARBON-DIOXIDE; CLIMATE-CHANGE; GAS-EXCHANGE; HYDRAULIC ARCHITECTURE; GROWTH TEMPERATURE; SEASONAL-CHANGES AB Longleaf pine (Pinus palustris Mill.), a species that once dominated the southeastern USA, is considered to be more drought tolerant than the principle plantation species in the South, loblolly pine (Pinus taeda L.) and slash pine (Pin us elliottii Engelm.), and so is predicted to better cope with increases in drought frequency associated with climate change. To determine if longleaf pine displays a more conservative water use strategy than the other two southern pine species, we examined diurnal patterns in leaf light-saturated photosynthesis, stomatal conductance, water use efficiency and leaf water potential (Psi(L)) over one growing season in a 50-year-old replicated field experiment. Short-term photosynthetic response to temperature was examined in August. No consistent differences among species in leaf gas exchange rates were observed, but Psi(L) was higher in longleaf pine compared to loblolly and slash pine across the growing season. Foliar delta C-13 measured at the end of the growing season was higher in longleaf pine than in loblolly pine but not slash pine. No temperature optimum of photosynthesis was observed in any species and photosynthesis did not respond to changing temperature. Based on leaf physiological traits, these results do not support the contention that longleaf pine has a more conservative leaf water use strategy than the other two pine species. The results do suggest that differences in hydraulic architecture or hydraulic efficiency may account for higher Psi(L) and perhaps greater drought tolerance in longleaf pine. (C) 2012 Elsevier B.V. All rights reserved. C1 [Samuelson, Lisa J.; Stokes, Tom A.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Johnsen, Kurt H.] US Forest Serv, USDA, So Res Stn, Res Triangle Pk, NC 28809 USA. RP Samuelson, LJ (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, 3301 SFWS Bldg, Auburn, AL 36849 USA. EM samuelj@auburn.edu FU USDA Forest Service, Southern Research Station; Center for Longleaf Pine Ecosystems at Auburn University FX This project was funded by the USDA Forest Service, Southern Research Station and the Center for Longleaf Pine Ecosystems at Auburn University. NR 69 TC 6 Z9 6 U1 3 U2 41 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2012 VL 274 BP 108 EP 115 DI 10.1016/j.foreco.2012.02.017 PG 8 WC Forestry SC Forestry GA 948PQ UT WOS:000304515500012 ER PT J AU Shinneman, DJ Palik, BJ Cornett, MW AF Shinneman, Douglas J. Palik, Brian J. Cornett, Meredith W. TI Can landscape-level ecological restoration influence fire risk? A spatially-explicit assessment of a northern temperate-southern boreal forest landscape SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire risk; Southern boreal forest; Northern temperate forest; LANDIS-II; Forest landscape simulation model; Wilderness ID SIMULATION-MODEL; SUCCESSION; MANAGEMENT; AREA; USA; MINNESOTA; CLIMATE; ONTARIO; LANDIS; DISTURBANCE AB Management strategies to restore forest landscapes are often designed to concurrently reduce fire risk. However, the compatibility of these two objectives is not always clear, and uncoordinated management among landowners may have unintended consequences. We used a forest landscape simulation model to compare the effects of contemporary management and hypothetical restoration alternatives on fire risk in northern temperate and southern boreal forests of the Border Lakes Region in Minnesota, USA, and Ontario, Canada. Six main model scenarios simulated different combinations of timber harvest, fire exclusion, wildland fire use, and prescribed fire. Mean fire risk values were calculated as a function of high risk fuel type occurrence, fire events, and windthrow events over model time, and were compared among scenarios and among major management areas. Our model results indicate that a continuation of contemporary management, with limited wildland fire use, would increase fire risk over time and lead to greater continuity of high-risk fuel types in parks and wilderness areas. Compared to the contemporary management scenario, greater use of wildland fire in a historical natural disturbance scenario and three alternative restoration scenarios resulted in less spatially aggregated high-risk fuels over time and lower long-term fire risk in parks and wilderness. Outside of parks and wilderness, prescribed fire with logging was effective at reducing fire risk on portions of the landscape in two restoration scenarios, largely by maintaining deciduous tree dominance and fire-tolerant red and white pine stands, and timber harvest alone maintained patches of less fire-prone deciduous forests in some scenarios. However, forest restoration and fire risk objectives were not always compatible, especially when restoration of fire-prone forest conflicted with the goal of reducing risk of large, severe fires. Both fire risk reduction and forest restoration objectives will benefit from spatially coordinated, landscape-level planning among landowners. Published by Elsevier B.V. C1 [Shinneman, Douglas J.; Palik, Brian J.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. [Shinneman, Douglas J.; Cornett, Meredith W.] Nature Conservancy, Duluth, MN 55802 USA. RP Shinneman, DJ (reprint author), US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Snake River Field Stn, 970 Lusk St, Boise, ID 83706 USA. EM dshinneman@usgs.gov FU U.S. Forest Service; U.S. Forest Service Northern Research Station; U.S. Geological Survey-Forest and Rangeland Ecosystem Science Center; Nature Conservancy; Minnesota Forest Resources Council FX Funding was provided mainly by the U.S. Forest Service National Fire Plan, with additional support by the U.S. Forest Service Northern Research Station, U.S. Geological Survey-Forest and Rangeland Ecosystem Science Center, The Nature Conservancy, and Minnesota Forest Resources Council. We thank Casey Souder for GIS contributions. NR 48 TC 7 Z9 7 U1 5 U2 60 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2012 VL 274 BP 126 EP 135 DI 10.1016/j.foreco.2012.02.030 PG 10 WC Forestry SC Forestry GA 948PQ UT WOS:000304515500014 ER PT J AU Elliott, KJ Vose, JM Knoepp, JD Clinton, BD AF Elliott, Katherine J. Vose, James M. Knoepp, Jennifer D. Clinton, Barton D. TI Restoration of shortleaf pine (Pinus echinata)-hardwood ecosystems severely impacted by the southern pine beetle (Dendroctonus frontalis) SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Prescribed burning; Fuel reduction; Structural equation modeling; Path analysis; Fire severity ID MIXED-OAK FORESTS; REPEATED PRESCRIBED FIRES; ABOVEGROUND BIOMASS; NITROGEN DYNAMICS; SOIL NUTRIENTS; CALIFORNIA SHRUBLANDS; UNDERSTORY VEGETATION; HERBACEOUS LAYER; HARDWOOD STANDS; PLANT DIVERSITY AB In the Southern Appalachian Mountains of eastern USA, pine-hardwood ecosystems have been severely impacted by the interactions of past land use, fire exclusion, drought, and southern pine beetle (SPB, Dendroctonus frontalis). We examined the effects of restoration treatments: burn only (BURN); cut + burn on dry sites (DC + B); cut + burn on sub-mesic sites (MC + B); and reference sites (REF; no cutting or burning) on shortleaf pine-hardwood forests. We also evaluated the effectiveness of seeding native bluestem grasses. Structural (down wood, live and dead standing trees, shrubs, herbaceous layer) and functional (forest floor mass, C, and N; soil C, N, P. and cations; and soil solution N and P) attributes were measured before and the first and second growing seasons after treatment. We used path analysis to test our conceptual model that restoration treatments will have direct and indirect effects on these ecosystems. Total aboveground mass loss ranged from 24.33 Mg ha(-1) on the BURN to 74.44 Mg ha(-1) on the DC + B treatment; whereas, REF gained 13.68 Mg ha(-1) between pre-burn and post-burn. Only DC + B sites had increased soil NO3-N, NH4-N Ca, Mg, and PO4-P and soil solution NO3-N, NH4-N, O-PO4 for several months. We found a significant increase in the density of oak species (Quercus alba, Q. coccinea, Q. montana, Q. rubra, and Q. velutina) on all burn treatments. However, oaks accounted for a smaller proportion of the total stem density than red maple, other tree species, and shrubs. The high densities of woody species other than oaks, coupled with the fast growth rates of some of these species, suggests that oaks will continue to be at a competitive disadvantage in these pine-hardwood communities through time, without further intervention. Pine regeneration was not improved on any of our burned sites with little to no recruitment of pines into the understory after two years and the pine saplings that were present before the burns were killed by fire on all sites. We found an increase in herbaceous layer cover and richness on all fire treatments. DC + B had higher bluestem grass cover than the other treatments, and it was the only treatment with increased bluestem grass cover between the first (2.96%, SE = 0.29) and second (6.88%, SE = 0.70) growing seasons. Our path model showed that fire severity explained a large proportion of the variation in overstory response; and fire severity and overstory response partially explained soil NO3-N. These variables, directly and indirectly, explained 64% of the variation in soil solution NO3-N at 30 cm soil depth (within the rooting zone for most plants). We found a good-fit path model for herbaceous layer response in the second growing season, where fire severity had direct effects on overstory and herbaceous layer responses and indirect effects on herbaceous layer response mediated through overstory response. Our path model explained 46% and 42% of the variation in herbaceous layer cover and species richness, respectively. Published by Elsevier B.V. C1 [Elliott, Katherine J.; Vose, James M.; Knoepp, Jennifer D.; Clinton, Barton D.] US Forest Serv, USDA, So Res Stn, Ctr Forest Watershed Res,Coweeta Hydrol Lab, Otto, NC 28763 USA. RP Elliott, KJ (reprint author), US Forest Serv, USDA, So Res Stn, Ctr Forest Watershed Res,Coweeta Hydrol Lab, Otto, NC 28763 USA. EM kelliott@fs.fed.us FU Joint Fire Science Program [05-2-1-29]; USDA Forest Service, Southern Research Station, Coweeta Hydrologic Laboratory; National Science Foundation [DEB-9632854, DEB-0218001] FX Special thanks to the Ocoee Ranger District, Cherokee National Forest for implementing the prescribed fires. We thank Patsy Clinton, Chris Sobek, Neal Muldoon, and Jim Gruhalla for assistance in field sampling and Cindi Brown and Carol Harper for chemical analyses of samples. Drs. Kevin Hiers and Stephen Brewer and two anonymous reviewers provided helpful comments on the manuscript. This research was funded by the Joint Fire Science Program (Project no. 05-2-1-29), USDA Forest Service, Southern Research Station, Coweeta Hydrologic Laboratory, and the Coweeta LTER project funded by National Science Foundation Grants DEB-9632854 and DEB-0218001. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the US Department of Agriculture of any product or service. NR 106 TC 5 Z9 5 U1 3 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUN 15 PY 2012 VL 274 BP 181 EP 200 DI 10.1016/j.foreco.2012.02.034 PG 20 WC Forestry SC Forestry GA 948PQ UT WOS:000304515500020 ER PT J AU Parsons, BN Porter, CJ Stavisky, JH Williams, NJ Birtles, RJ Miller, WG Hart, CA Gaskell, RM Dawson, S AF Parsons, B. N. Porter, C. J. Stavisky, J. H. Williams, N. J. Birtles, R. J. Miller, W. G. Hart, C. A. Gaskell, R. M. Dawson, S. TI Multilocus sequence typing of human and canine C. upsaliensis isolates SO VETERINARY MICROBIOLOGY LA English DT Article DE Dog; Campylobacter; C. upsaliensis; Zoonosis; MLST ID CAMPYLOBACTER-UPSALIENSIS; RISK-FACTORS; INFECTION; JEJUNI; COLI; GASTROENTERITIS; IDENTIFICATION; GENOTYPES; DIARRHEA; STRAINS AB Risk of Campylobacter infection in humans has been associated with many sources, including dogs. C. upsaliensis is the most common species found in canines, and has been occasionally isolated from symptomatic humans. This study aimed to investigate the genetic diversity of 41 C. upsaliensis isolates carried by dogs and from nine isolates carried by humans using Multilocus sequence typing (MLST). We identified considerable genetic diversity amongst the C. upsaliensis isolates from both dogs and humans, identifying 45 different sequence types (STs). All STs were new, apart from that of the reference strain. Only three STs were found in more than one isolate: ST-72 (2 isolates), ST-98 (2 isolates) and ST-104 (3 isolates). ST-104 was the only ST to be encountered in both dogs and humans. Thirty-one of the 45 STs were assigned to one of 13 clonal complexes (CCs). Four of these CCs contained STs originating from both humans and dogs. None of the CCs contained exclusively human isolates, and two isolates from dogs within the same kennel belonged to the same CC. The large amount of diversity found in both dog and human isolates of C. upsaliensis, combined with the relatively small database, made it difficult to assign strains to sources of infection. This emphasizes the need to increase the size of the database. Dog and human isolates occasionally grouped together, however there were insufficient human-derived isolates to determine whether or not dogs are a common source of infection. Although C upsaliensis infection is rare in humans, dogs still remain a potential source, and are therefore a possible zoonotic risk. Further work is needed to investigate the epidemiology of C. upsaliensis infection in humans. (C) 2012 Elsevier B.V. All rights reserved. C1 [Parsons, B. N.; Porter, C. J.; Stavisky, J. H.; Birtles, R. J.; Gaskell, R. M.] Inst Infect & Global Hlth, Sch Vet Sci, Neston CH64 7TE, England. [Hart, C. A.] Univ Liverpool, Dept Med Microbiol, Liverpool L69 3GA, Merseyside, England. [Miller, W. G.] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. RP Parsons, BN (reprint author), Univ Liverpool, Gastroenterol Unit, Inst Translat Med, Nuffield Bldg,Crown St, Liverpool L69 3GE, Merseyside, England. EM b.n.parsons@liv.ac.uk OI Stavisky, Jenny/0000-0002-1220-5690 FU Department for Environment, Food and Rural Affairs [OZ0612]; Intervet-Schering Plough FX This work was supported by funding from The Department for Environment, Food and Rural Affairs Research Contract number OZ0612 and Intervet-Schering Plough for Jenny Stavisky's Ph.D. The human strains of C upsaliensis were a kind gift from the Health Protection Agency. The authors would like to thank Ruth Ryvar for technical help, all the dog owners and the staff at the kennels. NR 36 TC 3 Z9 3 U1 1 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 J9 VET MICROBIOL JI Vet. Microbiol. PD JUN 15 PY 2012 VL 157 IS 3-4 BP 391 EP 397 DI 10.1016/j.vetmic.2011.12.035 PG 7 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 950IW UT WOS:000304643800016 PM 22266159 ER PT J AU Adeuya, A Price, NPJ AF Adeuya, Anthony Price, Neil P. J. TI Enumeration of hydroxyl groups of sugars and sugar alcohols by aqueous-based acetylation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry SO RAPID COMMUNICATIONS IN MASS SPECTROMETRY LA English DT Article ID BONE BIOMECHANICAL PROPERTIES; GAS-CHROMATOGRAPHY; XYLITOL; SILYLATION; POLYOLS; URINE; RATS AB RATIONALE The properties of carbohydrates are determined in part by the number and stereochemical arrangement of their hydroxyl groups. To facilitate their analysis, rapid methods are needed to identify and enumerate hydroxyl groups in sugars and polyalcohols, especially methods that are water-based. METHODS The present report details the results of an alternative method for identification and enumeration of hydroxyl groups in aqueous media. It employs vinyl acetate to selectively derivatize hydroxyl groups in analytes, followed by analysis of the reaction mixtures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). RESULTS The method has been applied to several single and multi-component mixtures of monosaccharides and polyalcohols. The O-acetylated products were analyzed without chromatographic separation or purification by MALDI-TOF-MS. The mass spectra revealed consecutive ion peaks that are separated by 42 mass units as a consequence of displacement of one hydroxyl hydrogen by one acetyl group. CONCLUSIONS A rapid and aqueous-based method is described to enumerate the hydroxyl groups in carbohydrates. The number of ion peaks due to derivatized products is determined by MALDI-TOF-MS, and corresponds to the number of free hydroxyl groups in the analyte. The method is applicable to both single and multi-component mixtures. Published 2011. This article is a US Government work and is in the public domain in the USA. C1 [Adeuya, Anthony; Price, Neil P. J.] ARS, USDA, NCAUR, Renewable Prod Technol Res Unit, Peoria, IL 61604 USA. RP Adeuya, A (reprint author), ARS, USDA, NCAUR, Renewable Prod Technol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM anthony.adeuya@fda.hhs.gov NR 21 TC 1 Z9 1 U1 2 U2 10 PU WILEY-BLACKWELL PI MALDEN PA COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA SN 0951-4198 J9 RAPID COMMUN MASS SP JI Rapid Commun. Mass Spectrom. PD JUN 15 PY 2012 VL 26 IS 11 BP 1372 EP 1376 DI 10.1002/rcm.6234 PG 5 WC Biochemical Research Methods; Chemistry, Analytical; Spectroscopy SC Biochemistry & Molecular Biology; Chemistry; Spectroscopy GA 936NL UT WOS:000303597100013 PM 22555931 ER PT J AU Silva, FRY Molina, JR Gonzalez-Caban, A Machuca, MAH AF Rodriguez y Silva, Francisco Ramon Molina, Juan Gonzalez-Caban, Armando Herrera Machuca, Miguel Angel TI Economic vulnerability of timber resources to forest fires SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Economic valuation; Fire economic losses; Fire prevention planning; Timber valuation ID PREDICTING POSTFIRE MORTALITY; PONDEROSA PINE; TREE MORTALITY; PRESCRIBED FIRE; SIERRA-NEVADA; DOUGLAS-FIR; CALIFORNIA; WILDFIRE; MANAGEMENT; RESISTANCE AB The temporal spatial planning of activities for a territorial fire management program requires knowing the value of forest ecosystems. In this paper we extend to and apply the economic valuation principle to the concept of economic vulnerability and present a methodology for the economic valuation of the forest production ecosystems. The forest vulnerability is analyzed from criteria intrinsically associated to the forest characterization, and to the potential behavior of surface fires. Integrating a mapping process of fire potential and analytical valuation algorithms facilitates the implementation of fire prevention planning. The availability of cartography of economic vulnerability of the forest ecosystems is fundamental for budget optimization, and to help in the decision making process. Published by Elsevier Ltd. C1 [Gonzalez-Caban, Armando] US Forest Serv, USDA, Pacific SW Res Stn, Riverside, CA 92507 USA. [Rodriguez y Silva, Francisco; Ramon Molina, Juan; Herrera Machuca, Miguel Angel] Univ Cordoba, Forest Fire Lab, Dept Forest Engn, E-14071 Cordoba, Spain. RP Gonzalez-Caban, A (reprint author), US Forest Serv, USDA, Pacific SW Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM agonzalezcaban@fs.fed.us RI MOLINA MARTINEZ, JUAN RAMON/K-8225-2014; Rodriguez y Silva, Francisco/M-2829-2014; HERRERA MACHUCA, MIGUEL ANGEL/M-3048-2014; OI HERRERA MACHUCA, MIGUEL ANGEL/0000-0002-1663-1750; Rodriguez y Silva, Francisco/0000-0002-9368-4688 NR 49 TC 6 Z9 6 U1 3 U2 17 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD JUN 15 PY 2012 VL 100 BP 16 EP 21 DI 10.1016/j.jenvman.2011.12.026 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA 920WE UT WOS:000302438600003 ER PT J AU Wang, SOY Camp, MJ Ehlenfeldt, MK AF Wang, Shiow Y. Camp, Mary J. Ehlenfeldt, Mark K. TI Antioxidant capacity and alpha-glucosidase inhibitory activity in peel and flesh of blueberry (Vaccinium spp.) cultivars SO FOOD CHEMISTRY LA English DT Article DE Antioxidant activity; Anthocyanins; Phenolics; alpha-Glucosidase; Blueberry peel; Blueberry flesh; Vaccinium species ID NATURAL ACYLATED ANTHOCYANINS; SCAVENGING CAPACITY; HYDROGEN-PEROXIDE; BERRY PHENOLICS; SINGLET OXYGEN; SMALL FRUITS; HYPERTENSION; ACID; GLYCOSIDES; OXIDATION AB This study was designed to evaluate cultivar variations in phenolic content, anthocyanin content, and antioxidant activity of peel and flesh; and to determine their potential inhibitory effects on alpha-glucosidase in 33 blueberry (Vaccinium species) cultivars, including 29 rabbiteye (Vaccinium ashei Reade) blueberries, two V. ashei hybrid derivatives, and two northern highbush (Vaccinium corymbosum L). The relation of phenolic, anthocyanin, and antioxidant activity to alpha-glucosidase inhibition in blueberries also was investigated. It was found that peel tissue possessed higher levels of total anthocyanins (TA), total phenolics (TP), antioxidant capacity, and cc-glucosidase inhibitory activity than flesh tissue in all blueberries tested. The percentage contributions of peel to whole berry on scavenging capacity against peroxyl free radicals (ROO center dot), hydroxyl radicals ((OH)-O-center dot), hydrogen peroxide (H2O2), and singlet oxygen (O-1(2)) radicals, were higher than those of flesh, even though the fruit contained much higher amounts of flesh than peel in terms of dry weight. Cultivars with high levels of phenolic compounds, antioxidant capacities, and alpha-glucosidase inhibitory activities could be selected for use in blueberry breeding programs to develop new lines with improved health benefits. Published by Elsevier Ltd. C1 [Wang, Shiow Y.] ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. [Camp, Mary J.] ARS, Biometr Consulting Serv, USDA, Beltsville, MD 20705 USA. [Ehlenfeldt, Mark K.] Rutgers State Univ, ARS, USDA, Genet Improvement Fruits & Vegetables Lab,Marucci, Chatsworth, NJ 08019 USA. RP Wang, SOY (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, USDA, Beltsville, MD 20705 USA. EM shiow.wang@ars.usda.gov NR 42 TC 25 Z9 29 U1 1 U2 53 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUN 15 PY 2012 VL 132 IS 4 BP 1759 EP 1768 DI 10.1016/j.foodchem.2011.11.134 PG 10 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 908SY UT WOS:000301513100020 ER PT J AU Yoo, J Kim, Y Yoo, SH Inglett, GE Lee, S AF Yoo, Jiyoung Kim, Yujeong Yoo, Sang-Ho Inglett, George E. Lee, Suyong TI Reduction of rutin loss in buckwheat noodles and their physicochemical characterisation SO FOOD CHEMISTRY LA English DT Article DE Buckwheat; Rutin; Quercetin; Noodles; Hydrothermal treatments ID FAGOPYRUM-ESCULENTUM; STARCH; PURIFICATION; FRACTIONS; TATARICUM; FOODS; FLOUR; SEEDS; DOUGH AB Tartary buckwheat was subjected to hydrothermal treatments for minimising rutin loss in buckwheat-based foods by water addition. When native buckwheat flour was mixed with water for 60 min, the rutin content was distinctly reduced from 3.74 g/100 g to 0.31 g/100 g, increasing the amount of quercetin. However, the rutin content remained constant and quercetin was hardly detected in hydrothermally-treated buckwheat flour. Also, when noodles were prepared with wheat and buckwheat flours (7:3, w/w), the noodle samples containing hydrothermally-treated buckwheat flour, showed higher amounts of rutin (more than 0.83 g/100 g) than the control noodle with native buckwheat flour (0.27 g/100 g). In addition, the use of hydrothermally-treated buckwheat flour gave less pasting parameters and lower viscoelastic properties. The noodle dough with hydrothermally-treated buckwheat flour also had greater water absorption and development time during mixing while the elongation stress of the noodle dough was reduced. (C) 2011 Elsevier Ltd. All rights reserved. C1 [Yoo, Jiyoung; Kim, Yujeong; Yoo, Sang-Ho; Lee, Suyong] Sejong Univ, Dept Food Sci & Technol, Seoul 143747, South Korea. [Yoo, Jiyoung; Kim, Yujeong; Yoo, Sang-Ho; Lee, Suyong] Sejong Univ, Carbohydrate Bioprod Res Ctr, Seoul 143747, South Korea. [Inglett, George E.] ARS, Cereal Prod & Food Sci Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Lee, S (reprint author), Sejong Univ, Dept Food Sci & Technol, 98 Gunja Dong, Seoul 143747, South Korea. EM suyonglee@sejong.ac.kr FU National Research Foundation of Korea (NRF); Ministry of Education, Science and Technology [2009-0087278] FX This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0087278). NR 24 TC 19 Z9 20 U1 7 U2 44 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUN 15 PY 2012 VL 132 IS 4 BP 2107 EP 2111 DI 10.1016/j.foodchem.2011.12.065 PG 5 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 908SY UT WOS:000301513100066 ER PT J AU Takeoka, GR Buttery, RG AF Takeoka, Gary R. Buttery, Ron G. TI Preparation and characterisation of 2-formyl-1,4,5,6-tetrahydropyridine, a compound with a cracker-like odour SO FOOD CHEMISTRY LA English DT Article DE 2-Formyl-1,4,5,6-tetrahydropyridine; Cracker-like odour; Popcorn-like odour; Aroma; Flavour; Infrared ID AROMA; POPCORN AB 2-Acetyltetrahydropyridine is an important flavour constituent that imparts roasty, popcorn-like, and cracker-like odours to foods, such as bread, rice, popcorn, taco shells and tortilla chips. We postulated that the homologue with a formyl group in the C-2 position, 2-formyltetrahydropyridine, might have similar desirable odour qualities. Synthesis of this compound was performed by oxidation of 2-piperidinemethanol with silver carbonate supported on Celite (R). This reaction produced a major volatile (46.9% of the total volatiles) with a retention index of 1023 on DB-1. GC-O revealed that the unknown had a strong cracker-like odour. The electron impact mass spectrum of the unknown had the following major ions: m/z 82, 111, 54, 68, 28, 43. The m/z 53-54 ratio and m/z 82-83 ratio were consistent with a 1,4,5,6-tetrahydropyridine ring structure. GC-FTIR of the unknown revealed an N-H stretching band at 3435 cm(-1) and a Fermi doublet at 2827 and 2713 cm(-1) that indicated the presence of an aldehyde. A strong carbonyl absorption band was present at 1703 cm(-1). The unknown was tentatively identified as 2-formyl-1,4,5,6-tetrahydropyridine, based on its retention index, odour quality, mass spectrum, and vapour phase infrared spectrum. Published by Elsevier Ltd. C1 [Takeoka, Gary R.; Buttery, Ron G.] ARS, Western Reg Res Serv, USDA, Albany, CA 94710 USA. RP Takeoka, GR (reprint author), ARS, Western Reg Res Serv, USDA, 800 Buchanan Street, Albany, CA 94710 USA. EM grt@pw.usda.gov NR 12 TC 1 Z9 1 U1 1 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUN 15 PY 2012 VL 132 IS 4 BP 2131 EP 2134 DI 10.1016/j.foodchem.2011.12.023 PG 4 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 908SY UT WOS:000301513100070 ER PT J AU Choudhary, RK Capuco, AV AF Choudhary, Ratan K. Capuco, Anthony V. TI In vitro expansion of the mammary stem/progenitor cell population by xanthosine treatment SO BMC CELL BIOLOGY LA English DT Article DE Mammary stem cell; Self renewal; Symmetric division; FNDC3B; Telomerase; Bovine ID STEM-CELLS; EXPRESSION; KINETICS; MODEL; SACK AB Background: Mammary stem cells are critical for growth and maintenance of the mammary gland and therefore are of considerable interest for improving productivity and efficiency of dairy animals. Xanthosine treatment has been demonstrated to promote expansion of putative mammary stem cells in vivo, and hepatic and hair follicle stem cells in vitro. In the latter, xanthosine promoted the symmetrical division of hepatic and hair follicle stem cells. The objective of this study was to determine if treating primary cultures of bovine mammary epithelial cells (MEC) with xanthosine increases the stem/progenitor cell population by promoting symmetrical division of mammary stem cells. Results: In vitro treatment with xanthosine increased the population of MEC during the exponential phase of cell growth, reducing the doubling time from 86 h in control cultures to 60 h in xanthosine-treated cultures. The bromodeoxyuridine (BrdU) labeling index and the proportion of MEC in S-phase both were increased by xanthosine treatment, indicating that increased cell accretion was due to increased cell proliferation. Analysis of daughter-pairs indicated that xanthosine promoted a shift from asymmetric to symmetric cell division. Moreover, the 30 % increase in symmetric cell division was concomitant with an increase in the proportion of MEC that were positive for a putative stem cell marker (FNDC3B) and a trend toward increased telomerase activity. These results suggest that xanthosine treatment in vitro can increase cell proliferation, promote symmetric cell division and enhance stem/progenitor cell activity. Conclusions: Xanthosine treatment increased the proliferation rate of bovine MEC in vitro. This was likely to be mediated by an increase in the proportion of stem/progenitor cells in the MEC population due to promotion of symmetrical stem cell division by xanthosine. C1 [Choudhary, Ratan K.; Capuco, Anthony V.] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Capuco, Anthony V.] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD USA. RP Capuco, AV (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. EM tony.capuco@ars.usda.gov FU CRIS from the USDA Agricultural Research Service [1265-3200-083-00D]; National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2008-35206-18825] FX This work was funded by CRIS no. 1265-3200-083-00D from the USDA Agricultural Research Service and by National Research Initiative Competitive Grant no. 2008-35206-18825 from the USDA National Institute of Food and Agriculture. NR 15 TC 9 Z9 10 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2121 J9 BMC CELL BIOL JI BMC Cell Biol. PD JUN 14 PY 2012 VL 13 AR 14 DI 10.1186/1471-2121-13-14 PG 8 WC Cell Biology SC Cell Biology GA 979UI UT WOS:000306845000001 PM 22698263 ER PT J AU Brown, PT Cordero, EC Mauget, SA AF Brown, Patrick T. Cordero, Eugene C. Mauget, Steven A. TI Reproduction of twentieth century intradecadal to multidecadal surface temperature variability in radiatively forced coupled climate models SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article ID CENTRAL UNITED-STATES; AIR-TEMPERATURE; WARMING HOLE; STREAMFLOW; SIMULATIONS; UNCERTAINTY; TRENDS; SOLAR AB Coupled Model Intercomparison Project 3 simulations that included time-varying radiative forcings were ranked according to their ability to consistently reproduce twentieth century intradecadal to multidecadal (IMD) surface temperature variability at the 5 degrees by 5 degrees spatial scale. IMD variability was identified using the running Mann-Whitney Z method. Model rankings were given context by comparing the IMD variability in preindustrial control runs to observations and by contrasting the IMD variability among the ensemble members within each model. These experiments confirmed that the inclusion of time-varying external forcings brought simulations into closer agreement with observations. Additionally, they illustrated that the magnitude of unforced variability differed between models. This led to a supplementary metric that assessed model ability to reproduce observations while accounting for each model's own degree of unforced variability. These two metrics revealed that discernable differences in skill exist between models and that none of the models reproduced observations at their theoretical optimum level. Overall, these results demonstrate a methodology for assessing coupled models relative to each other within a multimodel framework. C1 [Brown, Patrick T.; Cordero, Eugene C.] San Jose State Univ, Dept Meteorol & Climate Sci, San Jose, CA 95192 USA. [Mauget, Steven A.] ARS, Plant Stress & Water Conservat Lab, USDA, Lubbock, TX USA. RP Brown, PT (reprint author), San Jose State Univ, Dept Meteorol & Climate Sci, San Jose, CA 95192 USA. EM patrick.brown@sjsu.edu FU NSF's Faculty Early Career Development (CAREER) Program [ATM-0449996] FX We acknowledge the modeling groups, Program for Climate Model Diagnosis and Intercomparison (PCMDI), and WCRP's Working Group on Coupled Modeling (WGCM) for their roles in making available the WCRP CMIP 3 multimodel data set. Support for this data set is provided by the Office of Science, U. S. Department of Energy. This work was supported in part by NSF's Faculty Early Career Development (CAREER) Program, grant ATM-0449996 (E. Cordero and P. Brown). The USDA is an equal opportunity provider and employer. NR 38 TC 3 Z9 3 U1 2 U2 6 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X EI 2169-8996 J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD JUN 14 PY 2012 VL 117 AR D11116 DI 10.1029/2011JD016864 PG 15 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 959WH UT WOS:000305347900001 ER PT J AU Schnupf, U Momany, FA AF Schnupf, Udo Momany, Frank A. TI DFT Energy Optimization of a Large Carbohydrate: Cyclomaltohexaicosaose (CA-26) SO JOURNAL OF PHYSICAL CHEMISTRY B LA English DT Article ID X-RAY-STRUCTURE; V-AMYLOSE; ATOMIC-RESOLUTION; INCLUSION COMPLEXES; CRYSTAL-STRUCTURE; CYCLODEXTRINS; CONFORMATION; CYCLOAMYLOSE; SIMULATIONS; INITIO AB CA-26 is the largest cyclodextrin (546 atoms) for which refined X-ray structural data is available. Because of its size, 26 D-glucose residues, it is beyond the scope of study of most ab initio or density functional methods and to date has only been computationally examined using empirical force fields. The crystal structure of CA-26 is folded like a figure "8" into two 10 D-glucoses long antiparallel left-handed V (Verldeisterung)-type helices with a "band-flip" and "kink" at the top and bottom of the helices. DFTr methods were applied to CA-26 to determine if a carbohydrate molecule of this size could be geometry optimized and if it would show structural variances from application of dispersion and/or solvation. The DFTr reduced basis set method developed by the authors uses 4-31G on the carbon atoms of the glucose rings and 6-31+G* on all other atoms. B3LYP is the density functional used to successfully optimize CA-26, and other density functionals were then applied, including a self-consistent charge density functional tight binding (SCC-DFTB) method and the B97D (dispersion-corrected) and B97D-PCM (dispersion + implicit solvent) methods. Heavy atom coordinates were taken from one X-ray structure, fitted with hydrogen atoms, and geometry optimized using PM3 followed by B3LYP/6-31+G*/4-31G optimization. After optimization, the heavy atom rms deviation of the optimized DFTr (B3LYP) structure to the crystal structure was 0.89 angstrom, the rmsd of the B97D optimization was 1.38 angstrom, that for B97D-PCM was 0.95 angstrom, and that for SCC-DFTB was 0.94 angstrom. These results are very good considering that no explicit water molecules were included in the computational analysis and there were similar to 32-38 water molecules around each CA-26 molecule in the crystal structure. Tables of internal coordinates and puckering parameters were compared to the X-ray structures, and close correspondence was found. C1 [Momany, Frank A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Schnupf, Udo] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. RP Momany, FA (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM frank.momany@ars.usda.gov RI Schnupf, Udo/H-4703-2016 OI Schnupf, Udo/0000-0002-1457-1985 NR 21 TC 4 Z9 4 U1 3 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1520-6106 J9 J PHYS CHEM B JI J. Phys. Chem. B PD JUN 14 PY 2012 VL 116 IS 23 BP 6618 EP 6627 DI 10.1021/jp208927v PG 10 WC Chemistry, Physical SC Chemistry GA 959ZG UT WOS:000305356100005 PM 22148583 ER PT J AU Wu, ST Li, CJ Huang, W Li, WZ Li, RW AF Wu, Sitao Li, Congjun Huang, Wen Li, Weizhong Li, Robert W. TI Alternative Splicing Regulated by Butyrate in Bovine Epithelial Cells SO PLOS ONE LA English DT Article ID RNA-SEQ; HISTONE DEACETYLASES; HUMAN GENOME; GENE FUSION; PROHIBITIN; TRANSCRIPTION; PROLIFERATION; INHIBITORS; VARIANTS; TARGET AB As a signaling molecule and an inhibitor of histone deacetylases (HDACs), butyrate exerts its impact on a broad range of biological processes, such as apoptosis and cell proliferation, in addition to its critical role in energy metabolism in ruminants. This study examined the effect of butyrate on alternative splicing in bovine epithelial cells using RNA-seq technology. Junction reads account for 11.28 and 12.32% of total mapped reads between the butyrate-treated (BT) and control (CT) groups. 201,326 potential splicing junctions detected were supported by >= 3 junction reads. Approximately 94% of these junctions conformed to the consensus sequence (GT/AG) while similar to 3% were GC/AG junctions. No AT/AC junctions were observed. A total of 2,834 exon skipping events, supported by a minimum of 3 junction reads, were detected. At least 7 genes, their mRNA expression significantly affected by butyrate, also had exon skipping events differentially regulated by butyrate. Furthermore, COL5A3, which was induced 310-fold by butyrate (FDR <0.001) at the gene level, had a significantly higher number of junction reads mapped to Exon#8 (Donor) and Exon#11 (Acceptor) in BT. This event had the potential to result in the formation of a COL5A3 mRNA isoform with 2 of the 69 exons missing. In addition, 216 differentially expressed transcript isoforms regulated by butyrate were detected. For example, Isoform 1 of ORC1 was strongly repressed by butyrate while Isoform 2 remained unchanged. Butyrate physically binds to and inhibits all zinc-dependent HDACs except HDAC6 and HDAC10. Our results provided evidence that butyrate also regulated deacetylase activities of classical HDACs via its transcriptional control. Moreover, thirteen gene fusion events differentially affected by butyrate were identified. Our results provided a snapshot into complex transcriptome dynamics regulated by butyrate, which will facilitate our understanding of the biological effects of butyrate and other HDAC inhibitors. C1 [Wu, Sitao; Li, Weizhong] Univ Calif San Diego, Ctr Res Biol Syst, San Diego, CA 92103 USA. [Li, Congjun; Li, Robert W.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD USA. [Huang, Wen] N Carolina State Univ, Dept Genet, Raleigh, NC 27695 USA. RP Wu, ST (reprint author), Univ Calif San Diego, Ctr Res Biol Syst, San Diego, CA 92103 USA. EM robert.li@ars.usda.gov RI Li, Weizhong/A-9735-2008; Huang, Wen/B-5005-2011 OI Li, Weizhong/0000-0003-1804-9403; FU National Human Genome Research Institute (NHGRI) [R01HG005978] FX This study was partly supported by Award R01HG005978 to WL from the National Human Genome Research Institute (NHGRI). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NHGRI or the National Institutes of Health. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study NR 45 TC 5 Z9 6 U1 0 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 14 PY 2012 VL 7 IS 6 AR e39182 DI 10.1371/journal.pone.0039182 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WG UT WOS:000305347800053 PM 22720068 ER PT J AU Hansen, NC Allen, BL Baumhardt, RL Lyon, DJ AF Hansen, Neil C. Allen, Brett L. Baumhardt, R. Louis Lyon, Drew J. TI Research achievements and adoption of no-till, dryland cropping in the semi-arid US Great Plains SO FIELD CROPS RESEARCH LA English DT Article DE No-till; Dryland cropping systems; US Great Plains; Erosion ID WATER-USE EFFICIENCY; SOIL PHYSICAL-PROPERTIES; LEGUME GREEN FALLOW; WINTER-WHEAT; SPRING WHEAT; CANADIAN PRAIRIES; PRECIPITATION USE; ORGANIC-MATTER; PULSE CROPS; YIELD AB The Great Plains region of the United States is an area of widespread dryland crop production, with wheat being the dominant crop. Precipitation in the region ranges from 300 to 500 mm annually, with the majority of precipitation falling during hot summer months. The prevailing cropping system is a two-year rotation of wheat and summer fallow. The adoption of no-till practices has resulted in greater precipitation storage and use efficiency, which has led to greater cropping intensity, higher productivity, more diverse crop rotations, and improvements in soil properties. In Colorado, for example, a no-till rotation of winter wheat-maize-fallow increased total annualized grain yield by 75% compared to winter wheat-summer fallow. Soil erosion was reduced to just 25% of that from a conventional tillage wheat-summer fallow system. The primary challenge with reducing fallow frequency is the increase in yield variability and risk of crop failure. Improved approaches for choosing crop or fallow are being developed based on soil water content and forecasted weather. Development of alternative crops, crop rotations, and integrated livestock systems that are sustainable from both economic and ecological perspectives is an on-going effort. Other research is addressing adaptation of cropping practices to climate change and the potential for dryland biomass crop production for the developing biofuel industry. (C) 2012 Elsevier B.V. All rights reserved. C1 [Hansen, Neil C.] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Allen, Brett L.] USDA ARS, Agr Syst Res Unit, NPARL, Sidney, MT 59270 USA. [Baumhardt, R. Louis] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. [Lyon, Drew J.] Univ Nebraska, Panhandle Res & Extens Ctr, Scottsbluff, NE 69361 USA. RP Hansen, NC (reprint author), Colorado State Univ, Dept Soil & Crop Sci, 1170 Campus Delivery, Ft Collins, CO 80523 USA. EM neil.hansen@colostate.edu RI Yang, Yang/C-7464-2012 NR 59 TC 29 Z9 29 U1 4 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4290 J9 FIELD CROP RES JI Field Crop. Res. PD JUN 14 PY 2012 VL 132 SI SI BP 196 EP 203 DI 10.1016/j.fcr.2012.02.021 PG 8 WC Agronomy SC Agriculture GA 948OJ UT WOS:000304512200020 ER PT J AU Miller, MG Shukitt-Hale, B AF Miller, Marshall G. Shukitt-Hale, Barbara TI Berry Fruit Enhances Beneficial Signaling in the Brain SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Review DE berries; brain; aging signaling; antioxidants; anti-inflammatory; behavior ID MONOAMINE-OXIDASE-B; MILD COGNITIVE IMPAIRMENT; SERUM ANTIOXIDANT STATUS; TRANSFECTED COS-7 CELLS; CONCORD GRAPE JUICE; ALZHEIMERS-DISEASE; OXIDATIVE STRESS; BEHAVIORAL DEFICITS; BLUEBERRY EXTRACT; KAINIC ACID AB Increased lifespans have led to population aging and brought attention to healthcare concerns associated with old age. A growing body of preclinical and clinical research has identified neurological benefits associated with the consumption of berry fruits. In addition to their now well-known antioxidant effects, dietary supplementation with berry fruits also has direct effects on the brain. Intake of these fruits may help to prevent age-related neurodegeneration and resulting changes in cognitive and motor function. In cell and animal models, berry fruits mediate signaling pathways involved in inflammation and cell survival in addition to enhancing neuroplasticity, neurotransmission, and calcium buffering, all of which lead to attenuation of age- and pathology-related deficits in behavior. Recent clinical trials have extended these antioxidant, anti-inflammatory, and cognition-sparing effects to humans. This paper reviews recent evidence for the beneficial signaling effects of berry fruits on the brain and behavior. C1 [Miller, Marshall G.; Shukitt-Hale, Barbara] Tufts Univ, Human Nutr Res Ctr Aging, USDA ARS, Boston, MA 02111 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, USDA ARS, 711 Washington St, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov NR 102 TC 39 Z9 40 U1 9 U2 42 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 13 PY 2012 VL 60 IS 23 BP 5709 EP 5715 DI 10.1021/jf2036033 PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 956RU UT WOS:000305107600004 PM 22264107 ER PT J AU Lin, LZ Harnly, JM AF Lin, Long-Ze Harnly, James M. TI Quantitation of Flavanols, Proanthocyanidins, Isoflavones, Flavanones, Dihydrochalcones, Stilbenes, Benzoic Acid Derivatives Using Ultraviolet Absorbance after Identification by Liquid Chromatography-Mass Spectrometry SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE quantitation; hydroxybenzoic acids; flavanols; proanthocyanidins; flavanones; isoflavones; hydrolyzable tannins; UV; response factors; green tea ID PHENOLIC-COMPOUNDS; FOOD SOURCES; POLYPHENOLS; GREEN; TEA; BIOAVAILABILITY; TANNINS; LEAVES AB A general method was developed for the systematic quantitation of flavanols, proanthocyanidins, isoflavones, flavanones, dihydrochalcones, stilbenes, and hydroxybenzoic acid derivatives (mainly hydrolyzable tannins) based on UV band II absorbance arising from the benzoyl structure. The compound structures and the wavelength maximum were well correlated and were divided into four groups: the flavanols and proanthocyanidins at 278 nm, hydrolyzable tannins at 274 nm, flavanones at 288 nm, and isoflavones at 260 nm. Within each group, molar relative response factors (MRRFs) were computed for each compound based on the absorbance ratio of the compound and the group reference standard. Response factors were computed for the compounds as purchased (MRRF), after drying (MRRFD), and as the best predicted value (MRRFp). Concentrations for each compound were computed based on calibration with the group reference standard and the MRRFp. The quantitation of catechins, proanthocyanidins, and gallic acid derivatives in white tea was used as an example. C1 [Lin, Long-Ze; Harnly, James M.] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Lin, LZ (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM longze.lin@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health. NR 26 TC 15 Z9 15 U1 0 U2 32 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 13 PY 2012 VL 60 IS 23 BP 5832 EP 5840 DI 10.1021/jf3006905 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 956RU UT WOS:000305107600020 PM 22577798 ER PT J AU Queiroz, SCN Cantrell, CL Duke, SO Wedge, DE Nandula, VK Moraes, RM Cerdeira, AL AF Queiroz, Sonia C. N. Cantrell, Charles L. Duke, Stephen O. Wedge, David E. Nandula, Vijay K. Moraes, Rita M. Cerdeira, Antonio L. TI Bioassay-Directed Isolation and Identification of Phytotoxic and Fungitoxic Acetylenes from Conyza canadensis SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE horseweed; acetylenes; Conyza canadensis; phytotoxic; antifungal; fungicide; herbicide; allelopathy ID GROWTH; POLYACETYLENES; BIOAUTOGRAPHY; SUBSTANCES; ASTERACEAE; ASTEREAE; LACTONES; MODES; WEED AB Conyza canadensis (L.) Cronquist syn. (horseweed) is a problematic and invasive weed with reported allelopathic properties. To identify the phytotoxic constituents of the aerial parts, a systematic bioactivity-guided fractionation of the dichloromethane extract was performed. Three active enyne derivatives, (2Z,8Z)-matricaria acid methyl ester, (4Z,8Z)-matricaria lactone, and (4Z)-lachnophyllum lactone, were identified. The lactones inhibited growth of the monocot Agrostis stolonifera (bentgrass) and the dicot Lactuca sativa (lettuce) at 1 mg mL(-1), while the (2Z,8Z)-matricaria acid methyl ester was less active. In a dose response screening of the lactones for growth inhibitory activity against Lemna paucicostata, (4Z)-lachnophyllum lactone was the most active with an IC50 of 104 mu M, while the (4Z,8Z)-matricaria lactone was less active (IC50 of 220 mu M). In a fungal direct bioautography assay, the two lactones at 10 and 100 mu g/spot inhibited growth of the plant pathogenic fungi Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides. In a dose response screening of the lactones against six different plant pathogenic fungi, (4Z,8Z)-matricaria lactone was more active than the commercial fungicide azoxystrobin on Col. acutatum, Col. fragariae, and Col. gloeosporioides at 30 mu M and about as active as the commercial fungicide captan against Col. gloeosporioides, while (4Z)-lachnophyllum lactone was less active. C1 [Cantrell, Charles L.; Duke, Stephen O.; Wedge, David E.] Agr Res Serv, Natl Prod Utilizat Res Unit, USDA, University, MS 38677 USA. [Queiroz, Sonia C. N.; Cerdeira, Antonio L.] EMBRAPA Environm, BR-13820000 Jaguariuna, SP, Brazil. [Nandula, Vijay K.] Agr Res Serv, Crop Prod Syst Res Unit, USDA, Stoneville, MS 38776 USA. [Moraes, Rita M.] Univ Mississippi, Natl Ctr Nat Prod Res, Sch Pharm, University, MS 38677 USA. [Moraes, Rita M.] Univ Mississippi, Ctr Water & Wetland Resources, Abbeville, MS 38601 USA. RP Cantrell, CL (reprint author), Agr Res Serv, Natl Prod Utilizat Res Unit, USDA, University, MS 38677 USA. EM charles.cantrell@ars.usda.gov RI Queiroz, Sonia/G-9714-2012 FU EMBRAPA/Brazil; U.S. Department of Agriculture [58-6408-7-012] FX We acknowledge the partial financial support of EMBRAPA/Brazil and U.S. Department of Agriculture Agricultural Research Service Specific Cooperative Agreement No. 58-6408-7-012. NR 31 TC 13 Z9 14 U1 1 U2 51 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 13 PY 2012 VL 60 IS 23 BP 5893 EP 5898 DI 10.1021/jf3010367 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 956RU UT WOS:000305107600028 PM 22612410 ER PT J AU Hwang, T Band, LE Vose, JM Tague, C AF Hwang, Taehee Band, Lawrence E. Vose, James M. Tague, Christina TI Ecosystem processes at the watershed scale: Hydrologic vegetation gradient as an indicator for lateral hydrologic connectivity of headwater catchments SO WATER RESOURCES RESEARCH LA English DT Article ID LEAF-AREA INDEX; SOIL-MOISTURE; DISTRIBUTED HYDROLOGY; PARAMETER-ESTIMATION; UNGAUGED CATCHMENTS; NORTH-CAROLINA; FOREST; MODEL; FLOW; PATTERNS AB Lateral water flow in catchments can produce important patterns in water and nutrient fluxes and stores and also influences the long-term spatial development of forest ecosystems. Specifically, patterns of vegetation type and density along hydrologic flow paths can represent a signal of the redistribution of water and nitrogen mediated by lateral hydrologic flow. This study explores the use of emergent vegetation patterns to infer ecohydrologic processes and feedbacks in forested headwater catchments. We suggest a hydrologic gradient of vegetation density as an indicator of lateral connectivity within headwater catchments. We define the hydrologic vegetation gradient (HVG) as the increase of normalized difference vegetation index per unit increase of the topographic wetness index. HVG are estimated in different headwater catchments in the Coweeta Hydrologic Laboratory using summer IKONOS imagery. We use recession slope analysis with gauge data and a distributed ecohydrological model to characterize the patterns of seasonal flow regimes within the catchments. Correlations between HVG, catchment runoff, early recession parameters, and model parameters show the interactive role of vegetation and lateral hydrologic connectivity of systems in addition to climatic and geomorphic controls. This suggests that HVG effectively represents the level of partitioning between localized water use and lateral water flow along hydrologic flow paths, especially during the growing season. It also presents the potential to use simple remotely sensed hydrologic vegetation gradients as an indicator of lateral hydrologic connectivity to extrapolate recession behavior and key model parameters of distributed hydrological models for ungauged headwater catchments. C1 [Hwang, Taehee; Band, Lawrence E.] Univ N Carolina, Inst Environm, Chapel Hill, NC 27599 USA. [Band, Lawrence E.] Univ N Carolina, Dept Geog, Chapel Hill, NC 27599 USA. [Vose, James M.] USDA, Forest Serv, Coweeta Hydrol Lab, Otto, NC USA. [Tague, Christina] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. RP Hwang, T (reprint author), Univ N Carolina, Inst Environm, Campus Box 1105, Chapel Hill, NC 27599 USA. EM h7666@email.unc.edu FU National Science Foundation [DEB 0823293] FX We thank the Editor, Michael L. Roderick, and two anonymous reviewers for constructive comments. The research represented in this paper was supported by the National Science Foundation award to the Coweeta Long Term Ecologic Research project (DEB 0823293). The USDA Forest Service provides long-term streamflow and climate data, as well as access and facilities at the Coweeta Hydrologic Laboratory. NR 88 TC 23 Z9 23 U1 2 U2 95 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 J9 WATER RESOUR RES JI Water Resour. Res. PD JUN 13 PY 2012 VL 48 AR W06514 DI 10.1029/2011WR011301 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 959XY UT WOS:000305352700001 ER PT J AU Nonneman, DJ Brown-Brandl, T Jones, SA Wiedmann, RT Rohrer, GA AF Nonneman, Dan J. Brown-Brandl, Tami Jones, Shuna A. Wiedmann, Ralph T. Rohrer, Gary A. TI A defect in dystrophin causes a novel porcine stress syndrome SO BMC GENOMICS LA English DT Article ID MUSCULAR-DYSTROPHY; DILATED CARDIOMYOPATHY; MISSENSE MUTATIONS; MALIGNANT HYPERTHERMIA; HALOTHANE SENSITIVITY; HAL-1843-NORMAL PIGS; BINDING DOMAIN; ROD DOMAIN; GENE; PROTEIN AB Background: Losses of slaughter-weight pigs due to transport stress are both welfare and economic concerns to pork producers. Historically, the HAL-1843 mutation in ryanodine receptor 1 was considered responsible for most of the losses; however, DNA testing has effectively eliminated this mutation from commercial herds. We identified two sibling barrows in the USMARC swine herd that died from apparent symptoms of a stress syndrome after transport at 12 weeks of age. The symptoms included open-mouth breathing, skin discoloration, vocalization and loss of mobility. Results: We repeated the original mating along with sire-daughter matings to produce additional offspring. At 8 weeks of age, heart rate and electrocardiographs (ECG) were monitored during isoflurane anesthesia challenge (3% for 3 min). Four males from the original sire-dam mating and two males from a sire-daughter mating died after one minute of anesthesia. Animals from additional litters were identified as having a stress response, sometimes resulting in death, during regular processing and weighing. Affected animals had elevated plasma creatine phosphokinase (CPK) levels before and immediately after isoflurane challenge and cardiac arrhythmias. A pedigree containing 250 pigs, including 49 affected animals, was genotyped with the Illumina PorcineSNP60 Beadchip and only one chromosomal region, SSCX at 25.1-27.7 Mb over the dystrophin gene (DMD), was significantly associated with the syndrome. An arginine to tryptophan (R1958W) polymorphism in exon 41 of DMD was the most significant marker associated with stress susceptibility. Immunoblots of affected heart and skeletal muscle showed a dramatic reduction of dystrophin protein and histopathology of affected hearts indicated muscle fiber degeneration. Conclusions: A novel stress syndrome was characterized in pigs and the causative genetic factor most likely resides within DMD that results in less dystrophin protein and cardiac abnormalities that can lead to death under stressful conditions. The identification of predictive markers will allow us to determine the prevalence of this disease in commercial swine populations. This defect also provides a unique biomedical model for human cardiomyopathy associated with muscular dystrophy that may be superior to those available because of the similarities in anatomy and physiology and allow advances in gene therapies for human disease. C1 [Nonneman, Dan J.; Brown-Brandl, Tami; Jones, Shuna A.; Wiedmann, Ralph T.; Rohrer, Gary A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Nonneman, DJ (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. EM dan.nonneman@ars.usda.gov NR 41 TC 9 Z9 9 U1 2 U2 25 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 12 PY 2012 VL 13 AR 233 DI 10.1186/1471-2164-13-233 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 014YL UT WOS:000309411800001 PM 22691118 ER PT J AU Kudva, IT Griffin, RW Krastins, B Sarracino, DA Calderwood, SB John, M AF Kudva, Indira T. Griffin, Robert W. Krastins, Bryan Sarracino, David A. Calderwood, Stephen B. John, Manohar TI Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells SO BMC MICROBIOLOGY LA English DT Article DE O157; Rectoanal junction; LEE; Adherence; DMEM; GeLC-MS/MS ID III SECRETED PROTEINS; FEEDLOT CATTLE; MASS-SPECTROMETRY; TERMINAL RECTUM; O157-H7; O157H7; INFECTION; STRAINS; IDENTIFICATION; COLONIZATION AB Background: In this study, we present evidence that proteins encoded by the Locus of Enterocyte Effacement (LEE), considered critical for Escherichia coli O157 (O157) adherence to follicle-associated epithelial (FAE) cells at the bovine recto-anal junction (RAJ), do not appear to contribute to O157 adherence to squamous epithelial (RSE) cells also constituting this primary site of O157 colonization in cattle. Results: Antisera targeting intimin-gamma, the primary O157 adhesin, and other essential LEE proteins failed to block O157 adherence to RSE cells, when this pathogen was grown in DMEM, a culture medium that enhances expression of LEE proteins. In addition, RSE adherence of a DMEM grown O157 mutant lacking the intimin protein was comparable to that seen with its wild- type parent O157 strain grown in the same media. These adherence patterns were in complete contrast to that observed with HEp-2 cells (the adherence to which is mediated by intimin-gamma), assayed under same conditions. This suggested that proteins other than intimin-. that contribute to adherence to RSE cells are expressed by this pathogen during growth in DMEM. To identify such proteins, we defined the proteome of DMEM-grown- O157 (DMEM-proteome). GeLC-MS/MS revealed that the O157 DMEM-proteome comprised 684 proteins including several components of the cattle and human O157 immunome, orthologs of adhesins, hypothetical secreted and outer membrane proteins, in addition to the known virulence and LEE proteins. Bioinformatics-based analysis of the components of the O157 DMEM proteome revealed several new O157-specific proteins with adhesin potential. Conclusion: Proteins other than LEE and intimin-. proteins are involved in O157 adherence to RSE cells at the bovine RAJ. Such proteins, with adhesin potential, are expressed by this human pathogen during growth in DMEM. Ongoing experiments to evaluate their role in RSE adherence should provide both valuable insights into the O157-RSE interactions and new targets for more efficacious anti-adhesion O157 vaccines. C1 [Kudva, Indira T.] USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Griffin, Robert W.; Calderwood, Stephen B.; John, Manohar] Massachusetts Gen Hosp, Div Infect Dis, Boston, MA 02114 USA. [Krastins, Bryan; Sarracino, David A.] Harvard Partners Ctr Genet & Genom, Cambridge, MA 02139 USA. [Calderwood, Stephen B.; John, Manohar] Harvard Univ, Sch Med, Dept Med, Boston, MA 02114 USA. [Calderwood, Stephen B.] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02114 USA. RP Kudva, IT (reprint author), USDA ARS, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. EM Indira.Kudva@ars.usda.gov FU US National Institutes of Health Grant [R21 AI055963] FX This work was supported by a US National Institutes of Health Grant R21 AI055963 to I.T.K. Intellectual property rights for the O157 proteome identified in this study are held by Massachusetts General Hospital, Boston, MA. NR 34 TC 10 Z9 10 U1 0 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD JUN 12 PY 2012 VL 12 AR 103 DI 10.1186/1471-2180-12-103 PG 11 WC Microbiology SC Microbiology GA 994WV UT WOS:000307966100001 PM 22691138 ER PT J AU van Bochove, K van Schalkwijk, DB Parnell, LD Lai, CQ Ordovas, JM de Graaf, AA van Ommen, B Arnett, DK AF van Bochove, Kees van Schalkwijk, Daniel B. Parnell, Laurence D. Lai, Chao-Qiang Ordovas, Jose M. de Graaf, Albert A. van Ommen, Ben Arnett, Donna K. TI Clustering by Plasma Lipoprotein Profile Reveals Two Distinct Subgroups with Positive Lipid Response to Fenofibrate Therapy SO PLOS ONE LA English DT Article ID CORONARY-HEART-DISEASE; TYPE-2 DIABETES-MELLITUS; MAGNETIC-RESONANCE-SPECTROSCOPY; RANDOMIZED CONTROLLED-TRIAL; HDL-CHOLESTEROL; ARTERY-DISEASE; CARDIOVASCULAR EVENTS; INSULIN-RESISTANCE; SECONDARY PREVENTION; RISK AB Fibrates lower triglycerides and raise HDL cholesterol in dyslipidemic patients, but show heterogeneous treatment response. We used k-means clustering to identify three representative NMR lipoprotein profiles for 775 subjects from the GOLDN population, and study the response to fenofibrate in corresponding subgroups. The subjects in each subgroup showed differences in conventional lipid characteristics and in presence/absence of cardiovascular risk factors at baseline; there were subgroups with a low, medium and high degree of dyslipidemia. Modeling analysis suggests that the difference between the subgroups with low and medium dyslipidemia is influenced mainly by hepatic uptake dysfunction, while the difference between subgroups with medium and high dyslipidemia is influenced mainly by extrahepatic lipolysis disfunction. The medium and high dyslipidemia subgroups showed a positive, yet distinct lipid response to fenofibrate treatment. When comparing our subgroups to known subgrouping methods, we identified an additional 33% of the population with favorable lipid response to fenofibrate compared to a standard baseline triglyceride cutoff method. Compared to a standard HDL cholesterol cutoff method, the addition was 18%. In conclusion, by using constructing subgroups based on representative lipoprotein profiles, we have identified two subgroups of subjects with positive lipid response to fenofibrate therapy and with different underlying disturbances in lipoprotein metabolism. The total subgroup with positive lipid response to fenofibrate is larger than subgroups identified with baseline triglyceride and HDL cholesterol cutoffs. C1 [van Bochove, Kees; van Schalkwijk, Daniel B.; de Graaf, Albert A.; van Ommen, Ben] TNO, Dept Microbiol & Syst Biol, NL-3700 AJ Zeist, Netherlands. [van Bochove, Kees; van Schalkwijk, Daniel B.; de Graaf, Albert A.; van Ommen, Ben] TNO, Dept Microbiol & Syst Biol, Leiden, Netherlands. [van Schalkwijk, Daniel B.] Leiden Amsterdam Ctr Drug Res, Analyt Sci Div, Leiden, Netherlands. [van Schalkwijk, Daniel B.] Netherlands Bioinformat Ctr NBIC, Nijmegen, Netherlands. [Parnell, Laurence D.; Lai, Chao-Qiang; Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. RP van Bochove, K (reprint author), TNO, Dept Microbiol & Syst Biol, NL-3700 AJ Zeist, Netherlands. EM daan.vanschalkwijk@tno.nl OI de Graaf, Albert/0000-0002-1067-3570 FU NIH Heart, Lung and Blood Institute [U01HL72524]; European Nutrigenomics Organisation [DS08-008]; Netherlands Bioinformatics Centre, Biorange project [SP3.3.1] FX This study was supported by NIH Heart, Lung and Blood Institute grant U01HL72524, Genetic and Environmental Determinants of Triglycerides. KvB was supported by the European Nutrigenomics Organisation exchange grant DS08-008. DBvS was supported by The Netherlands Bioinformatics Centre, Biorange project SP3.3.1. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 9 Z9 9 U1 0 U2 2 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 12 PY 2012 VL 7 IS 6 AR e38072 DI 10.1371/journal.pone.0038072 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UJ UT WOS:000305340000010 PM 22719863 ER PT J AU Schetelig, MF Handler, AM AF Schetelig, Marc F. Handler, Alfred M. TI Strategy for enhanced transgenic strain development for embryonic conditional lethality in Anastrepha suspensa SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE apoptosis; insect pest management; sterile insect technique ID MEDITERRANEAN FRUIT-FLY; STERILE INSECT TECHNIQUE; CERATITIS-CAPITATA; DROSOPHILA-MELANOGASTER; DIPTERA TEPHRITIDAE; TRANSPOSON VECTOR; GENE-EXPRESSION; SPERM MARKING; TRANSFORMATION; MOSQUITO AB Here the first reproductive sterility system for the tephritid fruit fly pest, Anastrepha suspensa, is presented, based on lethality primarily limited to embryos heterozygous for a conditional lethal transgene combination. This tetracycline (Tet)-suppressible system uses a driver construct having the promoter from the newly isolated embryo-specific A. suspensa serendipity alpha gene linked to the Tet-transactivator. This was used to drive expression of a phosphomutated variant of the pro-apoptotic cell death gene, hid, from A. ludens, that was isolated, based on its identity to A. suspensa hid. The Alhid(Ala2) variant was shown to have the highest cell death activity in an in vitro A. suspensa cell death assay compared to the orthologous genes Ashid, Dmhid, and the variant Dmhid(Ala5). These cell death assays also allowed a determination of the most-efficient driver-effector cassette combinations for use in A. suspensa transformants, resulting in two hybrid strains exhibiting 100% lethality. One strain was 96% lethal in embryos in the absence of tetracycline, with none surviving past the first larval instar, which is critical for pests that are most damaging in late-larval stages. We demonstrate that the isolation and in vitro validation of species-specific promoters and lethal effector genes can greatly improve the efficiency of creating high-performance conditional lethality strains that may be extended to other insect pest species. C1 [Schetelig, Marc F.; Handler, Alfred M.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Schetelig, MF (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM marc.schetelig@usda.ars.gov FU International Atomic Energy Agency; USDA-National Institute of Food and Agriculture-Agriculture and Food Research Initiative FX We thank Shelley Olson (funded by United States Department of Agriculture (USDA)-Animal and Plant Health Inspection Service-Plant Protection and Quarantine) for excellent technical assistance, Seth Britch for assistance with statistical analysis, and Drs. Nirmala Xavier and Irina Hacker for comments on the manuscript. The project benefitted from discussions at International Atomic Energy Agency funded meetings for the Coordinated Research Project: The Use of Molecular Tools to Improve the Effectiveness of SIT. Funding is gratefully acknowledged from the USDA-National Institute of Food and Agriculture-Agriculture and Food Research Initiative. NR 35 TC 25 Z9 26 U1 1 U2 20 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 12 PY 2012 VL 109 IS 24 BP 9348 EP 9353 DI 10.1073/pnas.1203352109 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 962AL UT WOS:000305511300035 PM 22647610 ER PT J AU Han, EJ Heathman, GC Merwade, V Cosh, MH AF Han, Eunjin Heathman, Gary C. Merwade, Venkatesh Cosh, Michael H. TI Application of observation operators for field scale soil moisture averages and variances in agricultural landscapes SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil moisture; Upscaling; Cumulative distribution matching; Cedar Creek; Indiana ID REMOTE-SENSING FOOTPRINTS; TEMPORAL STABILITY; PROFILE RETRIEVAL; WATER CONTENT; ASSIMILATION; VARIABILITY; TIME; VALIDATION; PREDICTION; PRODUCTS AB Scale difference between in situ and remotely sensed soil moisture observations and model grid size has been an issue for validation of remote sensing data, soil moisture data assimilation and calibration of hydrologic models. This study aims to link two different scales of soil moisture estimates by upscaling single point measurements to field averages for representing field-scale agricultural areas (similar to 2 ha) located within the Upper Cedar Creek Watershed in northeastern Indiana. Several statistical methods, mainly focusing on cumulative distribution function (CDF) matching, are tested to upscale point measurements to spatially representative soil moisture. These transforming equations are termed observation operators. The CDF matching is found to be the most successful upscaling method followed by the mean relative difference method using temporally stable point measurements. This study also tests the temporal and spatial (horizontal and vertical) transferability of the different observation operators. Results indicate that the two observation operators from the CDF matching approach and the mean relative difference method using a temporally stable location are transferable in space, but not in time. Rainfall characteristic is most likely the dominant factor affecting the success of observation operator transferability. In addition, the CDF matching approach is found to be an effective method to deduce spatial variability (standard deviation) of soil moisture from single point measurements. (C) 2012 Elsevier B.V. All rights reserved. C1 [Han, Eunjin; Merwade, Venkatesh] Purdue Univ, Sch Civil Engn, W Lafayette, IN 47907 USA. [Heathman, Gary C.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Cosh, Michael H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Merwade, V (reprint author), Purdue Univ, Sch Civil Engn, W Lafayette, IN 47907 USA. EM Eunjin.Han@ars.usda.gov; Gary.Heathman@ars.usda.gov; vmerwade@purdue.edu; Michael.Cosh@ars.usda.gov RI Cosh, MIchael/A-8858-2015; Merwade, Venkatesh/D-4387-2015; Han, Eunjin /G-4121-2013 OI Cosh, MIchael/0000-0003-4776-1918; Merwade, Venkatesh/0000-0001-5518-2890; Han, Eunjin /0000-0001-6208-7410 NR 48 TC 9 Z9 9 U1 1 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 J9 J HYDROL JI J. Hydrol. PD JUN 11 PY 2012 VL 444 BP 34 EP 50 DI 10.1016/j.jhydrol.2012.03.035 PG 17 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA 956RY UT WOS:000305108000004 ER PT J AU White, SN Reynolds, JO Waldron, DF Schneider, DA O'Rourke, KI AF White, Stephen N. Reynolds, James O. Waldron, Daniel F. Schneider, David A. O'Rourke, Katherine I. TI Extended scrapie incubation time in goats singly heterozygous for PRNP S146 or K222 SO GENE LA English DT Article DE Prion; Gene; Transmissible spongiform encephalopathy; Susceptibility; Experimental challenge ID PRION PROTEIN GENE; CAPRINE PRP GENE; CLASSICAL SCRAPIE; NATURAL SCRAPIE; UNITED-STATES; CYPRUS GOATS; POLYMORPHISMS; ASSOCIATION; IDENTIFICATION; RESISTANCE AB Scrapie is the transmissible spongiform encephalopathy (TSE) of sheep and goats, and scrapie eradication in sheep is based in part on strong genetic resistance to classical scrapie. Goats may serve as a scrapie reservoir, and to date there has been no experimental inoculation confirming strong genetic resistance in goats. Two prion protein variants (amino acid substitutions S146 and K222) in goats have been significantly underrepresented in scrapie cases though present in scrapie-exposed flocks, and have demonstrated low cell-free protein conversion efficiency to the disease form (PrPD). To test degree of genetic resistance conferred in live animals with consistent exposure, we performed the first oral scrapie challenge of goats singly heterozygous for either PRNP S146 or K222. All N146-Q222 homozygotes became clinically scrapie positive by an average of 24 months, but all 5146 and K222 heterozygotes remain scrapie negative by both rectal biopsy and clinical signs at significantly longer incubation times (P<0.0001 for both comparisons). Recent reports indicate small numbers of S146 and K222 heterozygous goats have become naturally infected with scrapie, suggesting these alleles do not confer complete resistance in the heterozygous state but rather extend incubation. The oral challenge results presented here confirm extended incubation observed in a recent intracerebral challenge of K222 heterozygotes, and to our knowledge provide the first demonstration of extended incubation in S146 heterozygotes. These results suggest longer relevant trace-back histories in scrapie-eradication programs for animals bearing these alleles and strengthen the case for additional challenge experiments in both homozygotes to assess potential scrapie resistance. Published by Elsevier B.V. C1 [White, Stephen N.; Reynolds, James O.; Schneider, David A.; O'Rourke, Katherine I.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [White, Stephen N.; O'Rourke, Katherine I.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Waldron, Daniel F.] Texas Agrilife Res, San Angelo, TX 76901 USA. RP White, SN (reprint author), ARS, Anim Dis Res Unit, USDA, POB 646630, Pullman, WA 99164 USA. EM swhite@vetmed.wsu.edu; jreynold@vetmed.wsu.edu; d-waldron@tamu.edu; das@vetmed.wsu.edu; korourke@vetmed.wsu.edu RI Schneider, David/A-2833-2010; OI Schneider, David/0000-0001-9659-6731; White, Stephen N./0000-0001-9255-6054 FU USDA-ARS [5348-32000-030-00D] FX Thanks to Joan Rowe for animal access, Tom Truscott for biopsy screening, and Linda Hamburg for clinical monitoring. Thanks to Duane Chandler, Amy Hetrick, Ralph Horn, Emma Karel, and Lori Fuller for animal husbandry. This work was supported by USDA-ARS grant 5348-32000-030-00D. The funding source had no role in study design; in the collection, analysis, and interpretation of data: in writing the report; or in the decision to submit the article for publication. NR 18 TC 15 Z9 15 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 J9 GENE JI Gene PD JUN 10 PY 2012 VL 501 IS 1 BP 49 EP 51 DI 10.1016/j.gene.2012.03.068 PG 3 WC Genetics & Heredity SC Genetics & Heredity GA 949NX UT WOS:000304584000007 PM 22516690 ER PT J AU Goetz, SJ Bond-Lamberty, B Law, BE Hicke, JA Huang, C Houghton, RA McNulty, S O'Halloran, T Harmon, M Meddens, AJH Pfeifer, EM Mildrexler, D Kasischke, ES AF Goetz, S. J. Bond-Lamberty, B. Law, B. E. Hicke, J. A. Huang, C. Houghton, R. A. McNulty, S. O'Halloran, T. Harmon, M. Meddens, A. J. H. Pfeifer, E. M. Mildrexler, D. Kasischke, E. S. TI Observations and assessment of forest carbon dynamics following disturbance in North America SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article ID NET ECOSYSTEM PRODUCTION; SPRUCE FIRE CHRONOSEQUENCE; CANADIAN BOREAL FOREST; WESTERN UNITED-STATES; CHANGE TRACKER MODEL; COARSE WOODY DEBRIS; SOIL RESPIRATION; STAND AGE; PACIFIC-NORTHWEST; INTERIOR ALASKA AB Disturbance processes of various types substantially modify ecosystem carbon dynamics both temporally and spatially, and constitute a fundamental part of larger landscape-level dynamics. Forests typically lose carbon for several years to several decades following severe disturbance, but our understanding of the duration and dynamics of post-disturbance forest carbon fluxes remains limited. Here we capitalize on a recent North American Carbon Program disturbance synthesis to discuss techniques and future work needed to better understand carbon dynamics after forest disturbance. Specifically, this paper addresses three topics: (1) the history, spatial distribution, and characteristics of different types of disturbance (in particular fire, insects, and harvest) in North America; (2) the integrated measurements and experimental designs required to quantify forest carbon dynamics in the years and decades after disturbance, as presented in a series of case studies; and (3) a synthesis of the greatest uncertainties spanning these studies, as well as the utility of multiple types of observations (independent but mutually constraining data) in understanding their dynamics. The case studies-in the southeast U. S., central boreal Canada, U. S. Rocky Mountains, and Pacific Northwest-explore how different measurements can be used to constrain and understand carbon dynamics in regrowing forests, with the most important measurements summarized for each disturbance type. We identify disturbance severity and history as key but highly uncertain factors driving post-disturbance carbon source-sink dynamics across all disturbance types. We suggest that imaginative, integrative analyses using multiple lines of evidence, increased measurement capabilities, shared models and online data sets, and innovative numerical algorithms hold promise for improved understanding and prediction of carbon dynamics in disturbance-prone forests. C1 [Bond-Lamberty, B.] Univ Maryland, Pacific NW Natl Lab, Joint Global Change Res Inst, College Pk, MD 20740 USA. [Goetz, S. J.; Houghton, R. A.] Woods Hole Res Ctr, Falmouth, MA USA. [Law, B. E.; Harmon, M.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Hicke, J. A.] Univ Idaho, Dept Geog, Moscow, ID 83843 USA. [Huang, C.; Kasischke, E. S.] Univ Maryland, Dept Geog, College Pk, MD 20740 USA. [McNulty, S.] US Forest Serv, USDA, Eastern Forest Environm Threat Assessment Ctr, Raleigh, NC USA. [O'Halloran, T.] Sweet Briar Coll, Dept Environm Studies, Sweet Briar, VA 24595 USA. [Meddens, A. J. H.] Univ Idaho, Environm Sci Program, Moscow, ID 83843 USA. [Pfeifer, E. M.] US Forest Serv, USDA, Challis, ID USA. [Mildrexler, D.] Univ Montana, Univ Montana Numer Terradynam Simulat Grp NTSG, Coll Forestry & Conservat, Missoula, MT 59812 USA. RP Bond-Lamberty, B (reprint author), Univ Maryland, Pacific NW Natl Lab, Joint Global Change Res Inst, 5825 Univ Res Ct,Ste 3500, College Pk, MD 20740 USA. EM bondlamberty@pnl.gov RI Bond-Lamberty, Ben/C-6058-2008; Hicke, Jeff/M-9677-2013; Goetz, Scott/A-3393-2015; Law, Beverly/G-3882-2010 OI Bond-Lamberty, Ben/0000-0001-9525-4633; Goetz, Scott/0000-0002-6326-4308; Law, Beverly/0000-0002-1605-1203 FU NOAA [NA08OAR4310526, NA09OAR4310194]; NASA [NNX08AG13G]; NSF [0902056]; DOE Office of Biological and Environmental Research (BER) [DE-AC06-76RL01830]; Office of Science (BER), U.S. Department of Energy [DE-FG02-04ER63911]; USDA Forest Service Western Wildland Environmental Threat Assessment Center FX Authors S. J. Goetz and B. Bond-Lamberty contributed equally to this work. S. J. G. acknowledges support from NOAA Global Carbon Cycle Program grant NA08OAR4310526, NASA Carbon Cycle and Ecosystems program grant NNX08AG13G, and NSF Seasonality grant 0902056. B. B. L. was supported by the DOE Office of Biological and Environmental Research (BER), as part of BER's Terrestrial Ecosystem Science Program, under contract DE-AC06-76RL01830. B.E.L.'s contribution was supported by the Office of Science (BER), U.S. Department of Energy (grant DE-FG02-04ER63911) for AmeriFlux Science Team Research. J. A. H. and A. J. H. M. were supported by NOAA grant NA09OAR4310194 and the USDA Forest Service Western Wildland Environmental Threat Assessment Center. NR 167 TC 37 Z9 37 U1 4 U2 103 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUN 9 PY 2012 VL 117 AR G02022 DI 10.1029/2011JG001733 PG 17 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 957IG UT WOS:000305154100001 ER PT J AU Trible, BR Ramirez, A Suddith, A Fuller, A Kerrigan, M Hesse, R Nietfeld, J Guo, BQ Thacker, E Rowland, RRR AF Trible, Benjamin R. Ramirez, Alejandro Suddith, Andrew Fuller, Alexandra Kerrigan, Maureen Hesse, Richard Nietfeld, Jerome Guo, Baoqing Thacker, Eileen Rowland, Raymond R. R. TI Antibody responses following vaccination versus infection in a porcine circovirus-type 2 (PCV2) disease model show distinct differences in virus neutralization and epitope recognition SO VACCINE LA English DT Article DE PCV2; PRRSV; PCVAD; PCV2 vaccine; Antibody response ID MULTISYSTEMIC WASTING SYNDROME; RESPIRATORY SYNDROME VIRUS; EXPERIMENTAL REPRODUCTION; CONVENTIONAL PIGS; CAPSID PROTEIN; REPLICATION; DIAGNOSIS; LESIONS; RECOMBINATION; PATHOGENESIS AB Porcine circovirus associated disease (PCVAD) encompasses a group of syndromes linked to infection with porcine circovirus type 2 (PCV2). Based on the hypothesis that the immune responses to vaccination versus infection are quantitatively and qualitatively different, the objective of this study was to evaluate immunity, virus replication and disease protection in pigs vaccinated with PCV2 capsid protein (CP) and during infection. The disease model included dual infection with PCV2 and porcine reproductive and respiratory syndrome virus (PRRSV), a virus known to enhance disease progression and severity. The principal effect of PRRSV infection was to increase peak PCV2 viremia by almost 40-fold; however, PCV2 failed to show a reciprocal effect on PRRSV. In vaccinated pigs, there was no evidence of disease or PCV2 replication following dual virus challenge. Immunity following vaccination favored PCV2 neutralizing activity; whereas, PCV2 infection and disease produced high levels of non-neutralizing antibody, primarily directed against a polypeptide in the C-terminal region of CP. These results support the notion that the magnitude of the total antibody response cannot be used as a measure of protective immunity. Further-more, protection versus disease lies in the immunodominance of specific epitopes. Epitope specificity should be taken into consideration when designing PCV2 vaccines. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Trible, Benjamin R.; Suddith, Andrew; Fuller, Alexandra; Kerrigan, Maureen; Hesse, Richard; Nietfeld, Jerome; Rowland, Raymond R. R.] Kansas State Univ, Dept Diagnost Med & Pathobiol, Coll Vet Med, Manhattan, KS 66506 USA. [Ramirez, Alejandro] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA. [Guo, Baoqing] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. [Thacker, Eileen] USDA ARS, Beltsville, MD 20705 USA. RP Rowland, RRR (reprint author), Kansas State Univ, Dept Diagnost Med & Pathobiol, Coll Vet Med, 1800 Denison Ave, Manhattan, KS 66506 USA. EM browland@vet.k-state.edu FU National Pork Board [06-073, 07-208]; USDA AFRI [2009-35204-05290] FX This work was supported by National Pork Board Grant #06-073, #07-208 and USDA AFRI Grant #2009-35204-05290. NR 43 TC 14 Z9 16 U1 2 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD JUN 8 PY 2012 VL 30 IS 27 BP 4079 EP 4085 DI 10.1016/j.vaccine.2012.04.022 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 963YL UT WOS:000305660800017 PM 22521847 ER PT J AU Greenlee, JJ Smith, JD Greenlee, MHW Nicholson, EM AF Greenlee, Justin J. Smith, Jodi D. Greenlee, M. Heather West Nicholson, Eric M. TI Clinical and Pathologic Features of H-Type Bovine Spongiform Encephalopathy Associated with E211K Prion Protein Polymorphism SO PLOS ONE LA English DT Article ID CREUTZFELDT-JAKOB-DISEASE; TRANSMISSIBLE MINK ENCEPHALOPATHY; NATURAL SCRAPIE; SUFFOLK SHEEP; UNITED-STATES; ATYPICAL BSE; CATTLE; VARIANT; IDENTIFICATION; ACCUMULATION AB The majority of bovine spongiform encephalopathy (BSE) cases have been ascribed to the classical form of the disease. H-type and L-type BSE cases have atypical molecular profiles compared to classical BSE and are thought to arise spontaneously. However, one case of H-type BSE was associated with a heritable E211K mutation in the prion protein gene. The purpose of this study was to describe transmission of this unique isolate of H-type BSE when inoculated into a calf of the same genotype by the intracranial route. Electroretinograms were used to demonstrate preclinical deficits in retinal function, and optical coherence tomography was used to demonstrate an antemortem decrease in retinal thickness. The calf rapidly progressed to clinical disease (9.4 months) and was necropsied. Widespread distribution of abnormal prion protein was demonstrated within neural tissues by western blot and immunohistochemistry. While this isolate is categorized as BSE-H due to a higher molecular mass of the unglycosylated PrPSc isoform, a strong labeling of all 3 PrPSc bands with monoclonal antibodies 6H4 and P4, and a second unglycosylated band at approximately 14 kDa when developed with antibodies that bind in the C-terminal region, it is unique from other described cases of BSE-H because of an additional band 23 kDa demonstrated on western blots of the cerebellum. This work demonstrates that this isolate is transmissible, has a BSE-H phenotype when transmitted to cattle with the K211 polymorphism, and has molecular features that distinguish it from other cases of BSE-H described in the literature. C1 [Greenlee, Justin J.; Smith, Jodi D.; Nicholson, Eric M.] Agr Res Serv, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Greenlee, M. Heather West] Iowa State Univ, Ames, IA USA. RP Greenlee, JJ (reprint author), Agr Res Serv, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM justin.greenlee@ars.usda.gov FU congressionally appropriated funds FX This research was funded in its entirety by congressionally appropriated funds to the United States Department of Agriculture, Agriculture Research Service. The funders of the work did not influence study design, data collection and analysis, decision to publish, and preparation of the manuscript. NR 44 TC 14 Z9 14 U1 0 U2 3 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 8 PY 2012 VL 7 IS 6 AR e38678 DI 10.1371/journal.pone.0038678 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959TQ UT WOS:000305336800047 PM 22715405 ER PT J AU Yin, JG Qu, GG Cao, LL Li, QC Fetterer, R Feng, XS Liu, Q Wang, GP Qi, DM Zhang, XC Miramontes, E Jenkins, M Zhang, NS Tuo, WB AF Yin, Jigang Qu, Guanggang Cao, Lili Li, Qichang Fetterer, Raymond Feng, Xiaosheng Liu, Quan Wang, Guiping Qi, Dongmei Zhang, Xichen Miramontes, Eliseo Jenkins, Mark Zhang, Naisheng Tuo, Wenbin TI Characterization of Neospora caninum microneme protein 10 (NcMIC10) and its potential use as a diagnostic marker for neosporosis SO VETERINARY PARASITOLOGY LA English DT Article DE Neospora caninum; ELISA; Microneme protein; NcMIC10; Abortion ID TOXOPLASMA-GONDII; CIRCULATING ANTIGEN; INFECTION; IDENTIFICATION; INVASION; SERA; DOGS; TRANSMISSION; SECRETION; DISCHARGE AB Improvements in the serological diagnosis of neosporosis are needed to differentiate acute versus chronic Neospora caninum infections. In the present study, N. caninum microneme protein 10 (NcMIC10), similar to other microneme proteins, was shown to be released in a calcium-dependent manner. NcMIC10 may be discharged during active invasion of host cells by the parasite, and thus represent an excellent marker for the diagnosis of neosporosis. In order to test this hypothesis, recombinant NcMIC10 (rNcMIC10) was expressed in Escherichia coli, and polyclonal antibodies were generated against non-overlapping fragments of the protein. A capture ELISA was developed using these antibodies, and was found to be highly accurate and reproducible with a detection range of 10-10,000 pg/ml. The anti-rNcMIC10 antibodies used in this study did not cross-react with the Toxoplasma gondii antigens. NcMIC10 was detected by the ELISA in sera of 9 out of 10 goats (90%) experimentally infected with N. caninum tachyzoites. In general, goats infected with a lower dose (10(4)) of the parasite displayed a peak in NcMIC10 levels between weeks 4 and 5 post infection. Goats infected with a higher parasite dose (10(6)) displayed a more rapid increase in NcMIC10 levels. In most animals, NcMIC10 decreased to undetectable levels by week 6 post infection. This is the first circulating Neospora antigen-based assay which may complement the existing antibody-based assays for a rapid and cost-effective definitive diagnosis of neosporosis in livestock Published by Elsevier B.V. C1 [Yin, Jigang; Qu, Guanggang; Cao, Lili; Fetterer, Raymond; Feng, Xiaosheng; Miramontes, Eliseo; Jenkins, Mark; Tuo, Wenbin] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. [Yin, Jigang] Jilin Univ, Inst Zoonosis, Minist Educ, Key Lab Zoonosis Res, Changchun 130062, Peoples R China. [Cao, Lili; Feng, Xiaosheng; Zhang, Xichen; Zhang, Naisheng] Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Peoples R China. [Li, Qichang] Vaxim Inc, Gaithersburg, MD 20850 USA. [Qu, Guanggang] Shangdong Binzhou Acad Anim Sci & Vet Med, Binzhou City 256600, Shandong, Peoples R China. [Liu, Quan] Acad Mil Med Sci, Inst Vet Med, Changchun 130062, Peoples R China. [Wang, Guiping; Qi, Dongmei] Modern Agr Grp, Guangdong Inst, Guangzhou, Guangdong, Peoples R China. RP Tuo, WB (reprint author), ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. EM wenbin.tuo@ars.usda.gov NR 32 TC 7 Z9 10 U1 0 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD JUN 8 PY 2012 VL 187 IS 1-2 BP 28 EP 35 DI 10.1016/j.vetpar.2012.01.003 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 952NN UT WOS:000304799300005 PM 22284302 ER PT J AU Galbreath, KE Cook, JA Hoberg, EP AF Galbreath, Kurt E. Cook, Joseph A. Hoberg, Eric P. TI Climate's Role in Polar Bear Past SO SCIENCE LA English DT Letter C1 [Galbreath, Kurt E.] No Michigan Univ, Dept Biol, Marquette, MI 49855 USA. [Cook, Joseph A.] Univ New Mexico, Museum SW Biol, Albuquerque, NM 87131 USA. [Hoberg, Eric P.] ARS, USDA, Beltsville, MD 20705 USA. RP Galbreath, KE (reprint author), No Michigan Univ, Dept Biol, Marquette, MI 49855 USA. EM kgalbrea@nmu.edu NR 2 TC 0 Z9 0 U1 1 U2 25 PU AMER ASSOC ADVANCEMENT SCIENCE PI WASHINGTON PA 1200 NEW YORK AVE, NW, WASHINGTON, DC 20005 USA SN 0036-8075 J9 SCIENCE JI Science PD JUN 8 PY 2012 VL 336 IS 6086 BP 1230 EP 1230 PG 1 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 953WV UT WOS:000304905300016 PM 22679079 ER PT J AU Zhang, FM Chen, JM Pan, YD Birdsey, RA Shen, SH Ju, WM He, LM AF Zhang, Fangmin Chen, Jing M. Pan, Yude Birdsey, Richard A. Shen, Shuanghe Ju, Weimin He, Liming TI Attributing carbon changes in conterminous U.S. forests to disturbance and non-disturbance factors from 1901 to 2010 SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article ID NET PRIMARY PRODUCTION; WESTERN UNITED-STATES; SOIL ORGANIC-MATTER; LAND-USE CHANGE; CLIMATE-CHANGE; CANADA FORESTS; BOREAL FOREST; TERRESTRIAL ECOSYSTEMS; NITROGEN DEPOSITION; CO2 FERTILIZATION AB Recent climate variability (increasing temperature, droughts) and atmospheric composition changes (nitrogen deposition, rising CO2 concentration) along with harvesting, wildfires, and insect infestations have had significant effects on U. S. forest carbon (C) uptake. In this study, we attribute C changes in the conterminous U. S. forests to disturbance and non-disturbance factors with the help of forest inventory data, a continental stand age map, and an updated Integrated Terrestrial Ecosystem Carbon Cycle model (InTEC). We grouped factors into disturbances (harvesting, fire, insect infestation) and non-disturbances (CO2 concentration, N deposition, and climate variability) and estimated their subsequent impacts on forest regrowth patterns. Results showed that on average, the C sink in the conterminous U. S. forests from 1950 to 2010 was 206 Tg C yr(-1) with 87% (180 Tg C yr(-1)) of the sink in living biomass. Compared with the simulation of all factors combined, the estimated C sink would be reduced by 95 Tg C yr(-1) if disturbance factors were omitted, and reduced by 50 Tg C yr(-1) if non-disturbance factors were omitted. Our study also showed diverse regional patterns of C sinks related to the importance of driving factors. During 1980-2010, disturbance effects dominated the C changes in the South and Rocky Mountain regions, were almost equal to non-disturbance effects in the North region, and had minor effects compared with non-disturbance effects in the West Coast region. C1 [Zhang, Fangmin; Shen, Shuanghe] Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Coll Appl Meteorol, Nanjing 210044, Jiangsu, Peoples R China. [Zhang, Fangmin; Chen, Jing M.; He, Liming] Univ Toronto, Dept Geog, Toronto, ON M5S 1A1, Canada. [Pan, Yude; Birdsey, Richard A.] US Forest Serv, USDA, Newtown Sq, PA USA. [Ju, Weimin] Nanjing Univ, Int Inst Earth Syst Sci, Nanjing, Jiangsu, Peoples R China. RP Zhang, FM (reprint author), Nanjing Univ Informat Sci & Technol, Jiangsu Key Lab Agr Meteorol, Coll Appl Meteorol, Nanjing 210044, Jiangsu, Peoples R China. EM fmin.zhang@utoronto.ca RI Pan, Yude/F-6145-2015; OI He, Liming/0000-0003-4010-6814 FU National Basic Research Program of China (973 Program) [2010CB950702]; Jiangsu Graduate Innovation Program [CX09B_223Z]; Canadian Foundation for Climate and Atmospheric Sciences; USDA Forest Service [07-JV-11242300-114]; Priority Academic Program Development of Jiangsu Higher Education Institutions (PARO); Canadian Carbon Program FX This research was supported by a grant from National Basic Research Program of China (973 Program) (2010CB950702), Jiangsu Graduate Innovation Program (CX09B_223Z), Canadian Carbon Program funded by the Canadian Foundation for Climate and Atmospheric Sciences, grant 07-JV-11242300-114 from the USDA Forest Service, and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PARO). NR 84 TC 22 Z9 25 U1 3 U2 46 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0148-0227 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUN 7 PY 2012 VL 117 AR G02021 DI 10.1029/2011JG001930 PG 18 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 957IF UT WOS:000305154000001 ER PT J AU Kamo, K Kim, AY Park, SH Joung, YH AF Kamo, Kathryn Kim, Ah-Young Park, Se Hee Joung, Young Hee TI The 5 ' UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis SO BMC PLANT BIOLOGY LA English DT Article ID INTRON-MEDIATED ENHANCEMENT; TRANSIENT GENE-EXPRESSION; RICE RUBI3 GENE; TRANSGENE EXPRESSION; LEADER INTRON; 5'-UTR INTRON; PLANTS; MAIZE; CELLS; TRANSFORMATION AB Background: There are many non-cereal monocots of agronomic, horticultural, and biofuel importance. Successful transformation of these species requires an understanding of factors controlling expression of their genes. Introns have been known to affect both the level and tissue-specific expression of genes in dicots and cereal monocots, but there have been no studies on an intron isolated from a non-cereal monocot. This study characterizes the levels of GUS expression and levels of uidA mRNA that code for beta-glucuronidase (GUS) expression in leaves of Gladiolus and Arabidopsis using GUBQ1, a polyubiquitin promoter with a 1.234 kb intron, isolated from the non-cereal monocot Gladiolus, and an intronless version of this promoter. Results: Gladiolus and Arabidopsis were verified by Southern hybridization to be transformed with the uidA gene that was under control of either the GUBQ1 promoter (1.9 kb), a 5' GUBQ1 promoter missing its 1.234 kb intron (0.68 kb), or the CaMV 35 S promoter. Histochemical staining showed that GUS was expressed throughout leaves and roots of Gladiolus and Arabidopsis with the 1.9 kb GUBQ1 promoter. GUS expression was significantly decreased in Gladiolus and abolished in Arabidopsis when the 5'UTR-intron was absent. In Arabidopsis and Gladiolus, the presence of uidA mRNA was independent of the presence of the 5'UTR-intron. The 5'-UTR intron enhanced translation efficiency for both Gladiolus and Arabidopsis. Conclusions: The GUBQ1 promoter directs high levels of GUS expression in young leaves of both Gladiolus and Arabidopsis. The 5' UTR-intron from GUBQ1 resulted in a similar pattern of beta-glucuronidase translation efficiency for both species even though the intron resulted in different patterns of uidA mRNA accumulation for each species. C1 [Kim, Ah-Young; Park, Se Hee; Joung, Young Hee] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea. [Kamo, Kathryn] USDA, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Kamo, K (reprint author), Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea. EM yhjoung@jnu.ac.kr OI Kamo, Kathryn/0000-0001-6862-2410 FU Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ009052] FX We thank Anne O'Connor for technical assistance with the Gladiolus work. Mention of a trademark, proprietary product or vendor does not imply its approval to the exclusion of other products or vendors that may also be suitable. This work was partially supported by a grant from the Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea (PJ009052) to Y.H. Joung. NR 34 TC 10 Z9 11 U1 0 U2 14 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD JUN 6 PY 2012 VL 12 AR 79 DI 10.1186/1471-2229-12-79 PG 10 WC Plant Sciences SC Plant Sciences GA 981IJ UT WOS:000306960000001 PM 22672685 ER PT J AU Kaplan, F Alborn, HT von Reuss, SH Ajredini, R Ali, JG Akyazi, F Stelinski, LL Edison, AS Schroeder, FC Teal, PE AF Kaplan, Fatma Alborn, Hans T. von Reuss, Stephan H. Ajredini, Ramadan Ali, Jared G. Akyazi, Faruk Stelinski, Lukasz L. Edison, Arthur S. Schroeder, Frank C. Teal, Peter E. TI Interspecific Nematode Signals Regulate Dispersal Behavior SO PLOS ONE LA English DT Article ID DAUER PHEROMONE COMPONENT; SMALL-MOLECULE SIGNALS; CAENORHABDITIS-ELEGANS; ENTOMOPATHOGENIC NEMATODES; TEMPERATURE; EVOLUTION; PATTERNS; MODE AB Background: Dispersal is an important nematode behavior. Upon crowding or food depletion, the free living bacteriovorus nematode Caenorhabditis elegans produces stress resistant dispersal larvae, called dauer, which are analogous to second stage juveniles (J2) of plant parasitic Meloidogyne spp. and infective juveniles (IJ)s of entomopathogenic nematodes (EPN), e.g., Steinernema feltiae. Regulation of dispersal behavior has not been thoroughly investigated for C. elegans or any other nematode species. Based on the fact that ascarosides regulate entry in dauer stage as well as multiple behaviors in C. elegans adults including mating, avoidance and aggregation, we hypothesized that ascarosides might also be involved in regulation of dispersal behavior in C. elegans and for other nematodes such as IJ of phylogenetically related EPNs. Methodology/Principal Findings: Liquid chromatography-mass spectrometry analysis of C. elegans dauer conditioned media, which shows strong dispersing activity, revealed four known ascarosides (ascr#2, ascr#3, ascr#8, icas#9). A synthetic blend of these ascarosides at physiologically relevant concentrations dispersed C. elegans dauer in the presence of food and also caused dispersion of IJs of S. feltiae and J2s of plant parasitic Meloidogyne spp. Assay guided fractionation revealed structural analogs as major active components of the S. feltiae (ascr#9) and C. elegans (ascr#2) dispersal blends. Further analysis revealed ascr#9 in all Steinernema spp. and Heterorhabditis spp. infected insect host cadavers. Conclusions/Significance: Ascaroside blends represent evolutionarily conserved, fundamentally important communication systems for nematodes from diverse habitats, and thus may provide sustainable means for control of parasitic nematodes. C1 [Kaplan, Fatma; Alborn, Hans T.; Teal, Peter E.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. [von Reuss, Stephan H.; Schroeder, Frank C.] Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA. [von Reuss, Stephan H.; Schroeder, Frank C.] Cornell Univ, Dept Chem & Chem Biol, Ithaca, NY USA. [Ajredini, Ramadan; Edison, Arthur S.] Univ Florida, Dept Biochem & Mol Biol, Gainesville, FL 32610 USA. [Ajredini, Ramadan; Edison, Arthur S.] Univ Florida, Natl High Magnet Field Lab, Gainesville, FL USA. [Ali, Jared G.; Stelinski, Lukasz L.] Univ Florida, Dept Entomol & Nematol, Citrus Res & Educ Ctr, Lake Alfred, FL USA. [Akyazi, Faruk] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Akyazi, Faruk] Ordu Univ, Dept Plant Protect, Ordu, Turkey. RP Kaplan, F (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. EM fkaplan@ufl.edu RI Schroeder, Frank/H-5026-2012; OI Ali, Jareg/0000-0002-5794-9554; von Reuss, Stephan/0000-0003-4325-5495; Schroeder, Frank/0000-0002-4420-0237 FU National Institutes of Health [R01 GM085285] FX The work is partially supported by the National Institutes of Health R01 GM085285. No additional external funding was received for this study. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 29 TC 16 Z9 16 U1 4 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 6 PY 2012 VL 7 IS 6 AR e38735 DI 10.1371/journal.pone.0038735 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959WK UT WOS:000305348400091 PM 22701701 ER PT J AU Kim, SP Moon, E Nam, SH Friedman, M AF Kim, Sung Phil Moon, Eunpyo Nam, Seok Hyun Friedman, Mendel TI Hericium erinaceus Mushroom Extracts Protect Infected Mice against Salmonella Typhimurium-Induced Liver Damage and Mortality by Stimulation of Innate Immune Cells SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE Hericium erinaceus mushroom; macrophage; beta-glucan; Salmonella Typhimurium; liver necrosis; phagocytosis; mice; functional food ID NITRIC-OXIDE SYNTHASE; ANTIMICROBIAL ACTIVITY; WILD AB The present study investigated the antibacterial effect of four extracts from the fruitbody of the edible medicinal mushroom Hericium erinaceus (hot water extract, HIVE; microwave/50% ethanol extract, MANE; acid extract, ACE; and alkaline extract, AKE) against murine salmonellosis. The extracts had no effect on Salmonella ser. Typhimurium growth in culture. Nor were the extracts toxic to murine macrophage cells, RAW 264.7. HWE and MWE stimulated uptake of the bacteria into the macrophage cells as indicated by increased colony-forming unit (CPU) counts of the contents of the lysed macrophages infected with Salmonella Typhimurium for 30 and 60 min. Two hours postinfection, the bacterial counts increased in the macrophages, but 4 and 8 h postinfection the HWE- and MWE-treated cells showed greater activity against the bacteria than the control. HWE- and MANE-treated noninfected macrophages had altered morphology and elevated inducible nitric oxide (NO) synthase (iNOS) mRNA expression. In the presence of S. Typhimurium, iNOS mRNA expression was further increased, accompanied by an increase in NO production. Histology assays of the livers of mice infected with a sublethal dose (1 x 10(4) CFU) of S. Typhimurium showed that HWE and MWE, administered by daily intraperitoneal injection, protected against necrosis of the liver, a biomarker of in vivo salmonellosis. The lifespans of mice similarly infected with a lethal dose of S. Typhimurium (1 x 10(5) CFU) were significantly extended by HWE and MWE. beta-Glucan, known to stimulate the immune system, was previously found to be present in high amounts in the active extracts. These results suggest that the mushroom extract activities against bacterial infection in mice occur through the activation of innate immune cells. C1 [Moon, Eunpyo; Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Kim, Sung Phil] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Nam, SH (reprint author), Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. EM shnam@ajou.ac.kr; Mendel.Friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 FU Rural Development Administration, Republic of Korea [200901OFT113068122] FX This research received grant support from the Agenda Program (No. 200901OFT113068122), Rural Development Administration, Republic of Korea. NR 34 TC 23 Z9 23 U1 2 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 6 PY 2012 VL 60 IS 22 BP 5590 EP 5596 DI 10.1021/jf300897w PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 952ZY UT WOS:000304837700011 PM 22624604 ER PT J AU Walters, M Morrison, NI Claus, J Tang, GL Phillips, CE Young, R Zink, RT Alphey, L AF Walters, Michelle Morrison, Neil I. Claus, John Tang, Guolei Phillips, Caroline E. Young, Robin Zink, Richard T. Alphey, Luke TI Field Longevity of a Fluorescent Protein Marker in an Engineered Strain of the Pink Bollworm, Pectinophora gossypiella (Saunders) SO PLOS ONE LA English DT Article ID GELECHIIDAE; LEPIDOPTERA; SOOTY; FITNESS; GENETICS; EYE AB The cotton pest, pink bollworm (Pectinophora gossypiella (Saunders)), is a significant pest in most cotton-growing areas around the world. In southwestern USA and northern Mexico, pink bollworm is the target of the sterile insect technique (SIT), which relies on the mass-release of sterile pink bollworm adults to over-flood the wild population and thereby reduce it over time. Sterile moths reared for release are currently marked with a dye provided in their larval diet. There are concerns, however, that this marker fails from time to time, leading to sterile moths being misidentified in monitoring traps as wild moths. This can lead to expensive reactionary releases of sterile moths. We have developed a genetically marked strain that is engineered to express a fluorescent protein, DsRed2, which is easily screened under a specialised microscope. In order to test this marker under field conditions, we placed wild-type and genetically marked moths on traps and placed them in field cages. The moths were then screened, in a double-blind fashion, for DsRed2 fluorescence at regular intervals to determine marker reliability over time. The marker was shown to be robust in very high temperatures and generally proved reliable for a week or longer. More importantly, genotyping of moths on traps by PCR screening of the moths was 100% correct. Our findings indicate that this strain - and fluorescent protein markers in general - could make a valuable contribution to SIT. C1 [Walters, Michelle; Claus, John; Tang, Guolei; Zink, Richard T.] Anim Plant Hlth & Inspect Serv, Ctr Plant Hlth Sci & Technol, USDA, Phoenix, AZ USA. [Morrison, Neil I.; Phillips, Caroline E.; Alphey, Luke] Oxitec Ltd, Oxford, England. [Young, Robin] Univ Cambridge, Strangeways Res Lab, Cardiovasc Epidemiol Unit, Cambridge, England. [Zink, Richard T.] Anim Plant Hlth & Inspect Serv, Ctr Plant Hlth Sci & Technol, USDA, Ft Collins, CO USA. [Alphey, Luke] Univ Oxford, Dept Zool, Oxford OX1 3PS, England. RP Walters, M (reprint author), Anim Plant Hlth & Inspect Serv, Ctr Plant Hlth Sci & Technol, USDA, Phoenix, AZ USA. EM luke.alphey@oxitec.com NR 29 TC 9 Z9 9 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 5 PY 2012 VL 7 IS 6 AR e38547 DI 10.1371/journal.pone.0038547 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959VI UT WOS:000305343900056 PM 22693645 ER PT J AU Xuan, RC Yates, SR Ashworth, DJ Luo, LF AF Xuan, Richeng Yates, Scott R. Ashworth, Daniel J. Luo, Lifang TI Mitigating 1,3-Dichloropropene, Chloropicrin, and Methyl Iodide Emissions from Fumigated Soil with Reactive Film SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID AMMONIUM THIOSULFATE; ORGANIC AMENDMENTS; PLASTIC FILMS; DEGRADATION; VOLATILIZATION; BROMIDE; IRRIGATION; FERTILIZER; REDUCTION AB Implicated as a stratospheric ozone-depleting compound, methyl bromide (MeBr) is being phased out despite being considered to be the most effective soil fumigant. Its alternatives, i.e., 1,3-dichloropropene (1,3-D, which includes cis and trans isomers), chloropicrin (CP), and methyl iodide (MeI), have been widely used. High emissions of MeI from fumigated soil likely put farm workers and other bystanders at risk of adverse health effects. In this study, two types of constructed reactive film were tested for their ability to mitigate emissions of 1,3-D, CP, and Mel using laboratory permeability cells. Before activation, these films act as a physical barrier to trap fumigants leaving soil. After activation of the reactive layer containing ammonium thiosulfate solution, the films also act as a sink for the fumigants. Over 97% of trans-1,3-D and 99% of the cis-1,3-D, CP and MeI were depleted when they passed into the reactive film. Half-lives (t(1/2)) of cis-, trans-1,3-D, CP and MeI under activated reactive film were 1.2, 1.4, 1.6, and 2.0 h respectively at 40 degrees C. C1 [Xuan, Richeng; Yates, Scott R.; Ashworth, Daniel J.; Luo, Lifang] ARS, USDA, Salin Lab, Riverside, CA 92507 USA. [Xuan, Richeng; Ashworth, Daniel J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. RP Xuan, RC (reprint author), ARS, USDA, Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM richeng.xuan@ars.usda.gov; scott.yates@ars.usda.gov NR 25 TC 5 Z9 5 U1 0 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD JUN 5 PY 2012 VL 46 IS 11 BP 6143 EP 6149 DI 10.1021/es300388r PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA 952HJ UT WOS:000304783000066 PM 22534067 ER PT J AU Daniel, TC Muhar, A Arnberger, A Aznar, O Boyd, JW Chan, KMA Costanza, R Elmqvist, T Flint, CG Gobster, PH Gret-Regamey, A Lave, R Muhar, S Penker, M Ribe, RG Schauppenlehner, T Sikor, T Soloviy, I Spierenburg, M Taczanowska, K Tam, J von der Dunk, A AF Daniel, Terry C. Muhar, Andreas Arnberger, Arne Aznar, Olivier Boyd, James W. Chan, Kai M. A. Costanza, Robert Elmqvist, Thomas Flint, Courtney G. Gobster, Paul H. Gret-Regamey, Adrienne Lave, Rebecca Muhar, Susanne Penker, Marianne Ribe, Robert G. Schauppenlehner, Thomas Sikor, Thomas Soloviy, Ihor Spierenburg, Marja Taczanowska, Karolina Tam, Jordan von der Dunk, Andreas TI Contributions of cultural services to the ecosystem services agenda SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE natural capital; scenic beauty; cultural landscapes; tourism; spiritual value ID LANDSCAPE PREFERENCES; SCENIC BEAUTY; DECISION-MAKING; VALUATION; FOREST; VALUES; CLASSIFICATION; BIODIVERSITY; ECOLOGY; TOURISM AB Cultural ecosystem services (ES) are consistently recognized but not yet adequately defined or integrated within the ES framework. A substantial body of models, methods, and data relevant to cultural services has been developed within the social and behavioral sciences before and outside of the ES approach. A selective review of work in landscape aesthetics, cultural heritage, outdoor recreation, and spiritual significance demonstrates opportunities for operationally defining cultural services in terms of socioecological models, consistent with the larger set of ES. Such models explicitly link ecological structures and functions with cultural values and benefits, facilitating communication between scientists and stakeholders and enabling economic, multicriterion, deliberative evaluation and other methods that can clarify tradeoffs and synergies involving cultural ES. Based on this approach, a common representation is offered that frames cultural services, along with all ES, by the relative contribution of relevant ecological structures and functions and by applicable social evaluation approaches. This perspective provides a foundation for merging ecological and social science epistemologies to define and integrate cultural services better within the broader ES framework. C1 [Daniel, Terry C.] Univ Arizona, Sch Nat Resource & Environm, Tucson, AZ 85721 USA. [Muhar, Andreas; Arnberger, Arne; Schauppenlehner, Thomas; Taczanowska, Karolina] Univ Nat Resources & Life Sci, Dept Landscape Spatial & Infrastruct Sci, A-1190 Vienna, Austria. [Aznar, Olivier] Irstea, Evolut Usages Intervent Publ & Dev Espaces Ruraux, F-63172 Aubiere, France. [Boyd, James W.] Resources Future Inc, Washington, DC 20036 USA. [Chan, Kai M. A.; Tam, Jordan] Univ British Columbia, Inst Resources Environm & Sustainabil, Vancouver, BC V6T 1Z4, Canada. [Costanza, Robert] Portland State Univ, Inst Sustainable Solut, Portland, OR 97201 USA. [Elmqvist, Thomas] Stockholm Univ, Dept Syst Ecol, SE-10691 Stockholm, Sweden. [Elmqvist, Thomas] Stockholm Univ, Stockholm Resilience Ctr, SE-10691 Stockholm, Sweden. [Flint, Courtney G.] Univ Illinois, Urbana, IL 61801 USA. [Gobster, Paul H.] US Forest Serv, No Res Stn, USDA, Evanston, IL 60201 USA. [Gret-Regamey, Adrienne; von der Dunk, Andreas] Swiss Fed Inst Technol, Dept Civil Environm & Geomat Engn, CH-8093 Zurich, Switzerland. [Lave, Rebecca] Indiana Univ, Dept Geog, Bloomington, IN 47405 USA. [Muhar, Susanne] Univ Nat Resources & Life Sci, Dept Water Atmosphere & Environm, A-1180 Vienna, Austria. [Penker, Marianne] Univ Nat Resources & Life Sci, Dept Econ & Social Sci, A-1190 Vienna, Austria. [Ribe, Robert G.] Univ Oregon, Inst Sustainable Environm, Eugene, OR 97403 USA. [Sikor, Thomas] Univ E Anglia, Sch Int Dev, Norwich NR4 7TJ, Norfolk, England. [Soloviy, Ihor] Ukrainian Natl Forestry Univ, Inst Ecol Econ, UA-790057 Lvov, Ukraine. [Spierenburg, Marja] Vrije Univ Amsterdam, FSW Dept Org Studies, NL-1081 HV Amsterdam, Netherlands. RP Daniel, TC (reprint author), Univ Arizona, Sch Nat Resource & Environm, Tucson, AZ 85721 USA. EM tdaniel@u.arizona.edu RI Gobster, Paul/A-2826-2013; Spierenburg, Marja/F-8812-2013; Costanza, Robert/A-4912-2008; OI Gobster, Paul/0000-0002-8576-0310; Costanza, Robert/0000-0001-6348-8734; Penker, Marianne/0000-0002-5185-9558; Muhar, Andreas/0000-0001-7623-5859 FU US National Science Foundation; Austrian Ministry of Science and Research; Austrian Academy of Sciences; International Council of Science (ICSU); Research Platform Eisenwurzen within the International Long-Term Ecological Research Network FX The international collaboration that produced this paper was initiated by the Kerner-von-Marilaun Workshop (November 2-6, 2009) in Lunz am See, Austria, sponsored by the US National Science Foundation, the Austrian Ministry of Science and Research, and the Austrian Academy of Sciences. We also acknowledge the support of the workshop by the International Council of Science (ICSU) and the Research Platform Eisenwurzen within the International Long-Term Ecological Research Network. NR 139 TC 250 Z9 260 U1 39 U2 327 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD JUN 5 PY 2012 VL 109 IS 23 BP 8812 EP 8819 DI 10.1073/pnas.1114773109 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 955BC UT WOS:000304991100017 PM 22615401 ER PT J AU Cui, Y Lee, MY Huo, NX Bragg, J Yan, LJ Yuan, C Li, C Holditch, SJ Xie, JZ Luo, MC Li, DW Yu, JL Martin, J Schackwitz, W Gu, YQ Vogel, JP Jackson, AO Liu, ZY Garvin, DF AF Cui, Yu Lee, Mi Yeon Huo, Naxin Bragg, Jennifer Yan, Lijie Yuan, Cheng Li, Cui Holditch, Sara J. Xie, Jingzhong Luo, Ming-Cheng Li, Dawei Yu, Jialin Martin, Joel Schackwitz, Wendy Gu, Yong Qiang Vogel, John P. Jackson, Andrew O. Liu, Zhiyong Garvin, David F. TI Fine Mapping of the Bsr1 Barley Stripe Mosaic Virus Resistance Gene in the Model Grass Brachypodium distachyon SO PLOS ONE LA English DT Article ID AGROBACTERIUM-MEDIATED TRANSFORMATION; LINKAGE MAP; GENOME; WHEAT; RICE; PATHOGENICITY; DETERMINANTS; SYSTEM; MOVEMENT; SATIVA AB The ND18 strain of Barley stripe mosaic virus (BSMV) infects several lines of Brachypodium distachyon, a recently developed model system for genomics research in cereals. Among the inbred lines tested, Bd3-1 is highly resistant at 20 to 25 degrees C, whereas Bd21 is susceptible and infection results in an intense mosaic phenotype accompanied by high levels of replicating virus. We generated an F-6:7 recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21 and used the RILs, and an F-2 population of a second Bd21 X Bd3-1 cross to evaluate the inheritance of resistance. The results indicate that resistance segregates as expected for a single dominant gene, which we have designated Barley stripe mosaic virus resistance 1 (Bsr1). We constructed a genetic linkage map of the RIL population using SNP markers to map this gene to within 705 Kb of the distal end of the top of chromosome 3. Additional CAPS and Indel markers were used to fine map Bsr1 to a 23 Kb interval containing five putative genes. Our study demonstrates the power of using RILs to rapidly map the genetic determinants of BSMV resistance in Brachypodium. Moreover, the RILs and their associated genetic map, when combined with the complete genomic sequence of Brachypodium, provide new resources for genetic analyses of many other traits. C1 [Cui, Yu; Yan, Lijie; Yuan, Cheng; Li, Cui; Xie, Jingzhong; Li, Dawei; Yu, Jialin; Liu, Zhiyong] China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China. [Cui, Yu; Lee, Mi Yeon; Holditch, Sara J.; Jackson, Andrew O.] Univ Calif Berkeley, Dept Plant & Microbiol, Berkeley, CA 94720 USA. [Huo, Naxin; Bragg, Jennifer; Gu, Yong Qiang; Vogel, John P.] USDA ARS Western Reg Res Ctr, Albany, CA USA. [Luo, Ming-Cheng] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Martin, Joel; Schackwitz, Wendy] US DOE Joint Genome Inst, Walnut Creek, CA USA. [Garvin, David F.] Univ Minnesota, USDA ARS Plant Sci Res Unit, St Paul, MN 55108 USA. [Garvin, David F.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. RP Cui, Y (reprint author), China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China. EM andyoj@berkeley.edu; zhiyongliu@cau.edu.cn RI Luo, Ming-Cheng/C-5600-2011; Li, Dawei /I-8751-2016; OI Li, Dawei /0000-0003-4133-1263; Luo, Ming-Cheng/0000-0002-9744-5887; Vogel, John/0000-0003-1786-2689 FU China Scholarship Council; National Natural Science Foundation of China [30971780, 31030056]; 863 project [2011AA100104]; MOA [2009ZX08009-048B]; Program of Introducing Talents of Discipline to Universities [111-2-03]; United States Department of Agriculture [3640-21000-021-00D]; Office of Science (BER), U.S. Department of Energy [DE-AI02-07ER64452, DE-SC0001526]; Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]; [2012SKLAB01-7]; [CSREES-2008-35319-19225] FX This work was supported by a China Scholarship Council Award to YC, by the National Natural Science Foundation of China (30971780, 31030056), 2012SKLAB01-7, 863 project 2011AA100104, MOA projects 2009ZX08009-048B, and the Program of Introducing Talents of Discipline to Universities (111-2-03) to ZL, by the United States Department of Agriculture (CRIS project no. 3640-21000-021-00D), and Competitive Grant CSREES-2008-35319-19225 to AOJ, and by the Office of Science (BER), U.S. Department of Energy (Interagency Agreement nos. DE-AI02-07ER64452 and DE-SC0001526). Work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 19 Z9 21 U1 2 U2 31 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 4 PY 2012 VL 7 IS 6 AR e38333 DI 10.1371/journal.pone.0038333 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 959UU UT WOS:000305341700059 PM 22675544 ER PT J AU Hoover, CM Smith, JE AF Hoover, Coeli M. Smith, James E. TI Site Productivity and Forest Carbon Stocks in the United States: Analysis and Implications for Forest Offset Project Planning SO FORESTS LA English DT Article DE Forest Inventory and Analysis database; soil moisture regime; aboveground live biomass carbon; site productivity ID ABOVEGROUND BIOMASS; NUTRIENT ACCUMULATION; BRITISH-COLUMBIA; AGE SEQUENCE; STANDS; REGIMES; PINE; ZONE AB The documented role of United States forests in sequestering carbon, the relatively low cost of forest-based mitigation, and the many co-benefits of increasing forest carbon stocks all contribute to the ongoing trend in the establishment of forest-based carbon offset projects. We present a broad analysis of forest inventory data using site quality indicators to provide guidance to managers planning land acquisition for forest-based greenhouse gas mitigation projects. Specifically, we summarize two condition class indicators of site productivity within the FIA forest inventory database-PHYSCLCD and SITECLCD-as they relate to current aboveground live tree carbon stocks. Average carbon density is higher on more productive sites, but compared to the overall variability among sites, the differences are relatively small for all but the highest and lowest site classes. Some minor differences in eastern-versus western-forests were apparent in terms of how carbon on the least productive sites differed from most other forest land over time. Overall results suggest that xeric sites in most regions as well as sites that correspond to the lowest, non-productive classifications of forest land should preferentially not be used forestry-based greenhouse gas mitigation projects, but all other forest areas appear to be suitable. C1 [Hoover, Coeli M.; Smith, James E.] US Forest Serv, USDA, No Res Stn, Durham, NH USA. RP Hoover, CM (reprint author), US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH USA. EM choover@fs.fed.us; jsmith11@fs.fed.us NR 18 TC 1 Z9 1 U1 0 U2 10 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2012 VL 3 IS 2 BP 283 EP 299 DI 10.3390/f3020283 PG 17 WC Forestry SC Forestry GA 096OU UT WOS:000315414900008 ER PT J AU Moore, PT DeRose, RJ Long, JN van Miegroet, H AF Moore, Patrick T. DeRose, R. Justin Long, James N. van Miegroet, Helga TI Using Silviculture to Influence Carbon Sequestration in Southern Appalachian Spruce-Fir Forests SO FORESTS LA English DT Article DE additionality; carbon sequestration; fire and fuels extension; forest carbon accounting; Forest Vegetation Simulator; silviculture; spruce-fir ID GREAT-SMOKY-MOUNTAINS; RED SPRUCE; LAND MANAGEMENT; NATIONAL-PARK; PRODUCTS; DENSITY; OVERSTORY; MODEL; SINKS AB Enhancement of forest growth through silvicultural modification of stand density is one strategy for increasing carbon (C) sequestration. Using the Fire and Fuels Extension of the Forest Vegetation Simulator, the effects of even-aged, uneven-aged and no-action management scenarios on C sequestration in a southern Appalachian red spruce-Fraser fir forest were modeled. We explicitly considered C stored in standing forest stocks and the fate of forest products derived from harvesting. Over a 100-year simulation period the even-aged scenario (250 Mg C ha(-1)) outperformed the no-action scenario (241 Mg C ha(-1)) in total carbon (TC) sequestered. The uneven-aged scenario approached 220 Mg C ha(-1), but did not outperform the no-action scenario within the simulation period. While the average annual change in C (AAC) of the no-action scenario approached zero, or carbon neutral, during the simulation, both the even-aged and uneven-aged scenarios surpassed the no-action by year 30 and maintained positive AAC throughout the 100-year simulation. This study demonstrates that silvicultural treatment of forest stands can increase potential C storage, but that careful consideration of: (1) accounting method (i.e., TC versus AAC); (2) fate of harvested products and; (3) length of the planning horizon (e. g., 100 years) will strongly influence the evaluation of C sequestration. C1 [Moore, Patrick T.] US Forest Serv, Cedar City, UT 84721 USA. [DeRose, R. Justin] US Forest Serv, Rocky Mt Res Stn, Ogden, UT 84401 USA. [Long, James N.; van Miegroet, Helga] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Long, James N.; van Miegroet, Helga] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. RP DeRose, RJ (reprint author), US Forest Serv, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. EM ptmoore@fs.fed.us; rjderose@fs.fed.us; james.long@usu.edu; helga.vanmiegroet@usu.edu RI Van Miegroet, Helga/A-1925-2012 FU USDA National Research Initiative Competitive Grants Program [97-35101-4314]; USGS Biological Research Division [1434 HQ97-RV-01555 RWO27, RWO34]; Utah Agricultural Experiment Station, Utah State University; Tennessee Valley Authority's Public Power Institute FX We graciously thank Niki Nicholas, Alan Mays and Larry Shelton, from the Tennessee Valley Authority (TVA) for all of their logistical assistance; Chloe Tewksbury, Faye Tewksbury and Mike Mancusi for their help in the field. We also thank TVA and EPA for the use of the forest inventory data sets. Funding for data collection was provided by the USDA National Research Initiative Competitive Grants Program (Grant No. 97-35101-4314 to Utah State University), the USGS Biological Research Division (Cooperative Agreement No. 1434 HQ97-RV-01555 RWO27 and RWO34 to the Utah Cooperative Fish & Wildlife Research Unit), and Tennessee Valley Authority's Public Power Institute. This study was supported by the Utah Agricultural Experiment Station, Utah State University and approved as AES Publication No. 8414. This article was prepared in part by an employee of the US Forest Service as part of official duties and therefore is in the public domain. NR 56 TC 2 Z9 2 U1 2 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2012 VL 3 IS 2 BP 300 EP 316 DI 10.3390/f3020300 PG 17 WC Forestry SC Forestry GA 096OU UT WOS:000315414900009 ER PT J AU Hanula, JL Ulyshen, MD Wade, DD AF Hanula, James L. Ulyshen, Michael D. Wade, Dale D. TI Impacts of Prescribed Fire Frequency on Coarse Woody Debris Volume, Decomposition and Termite Activity in the Longleaf Pine Flatwoods of Florida SO FORESTS LA English DT Article DE coarse woody debris; decay; deadwood; decomposers; fire frequency; large fuel consumption; termites; Isoptera ID SUBTERRANEAN TERMITES; RAIN-FOREST; VEGETATION; ASSEMBLAGES; ECOSYSTEMS; EXCLUSION; ISOPTERA; MALAYSIA; SAVANNA; ECOLOGY AB Longleaf pine (Pinus palustris) ecosystems have been reduced dramatically throughout their range. Prescribed burning is considered the best way to restore and maintain plant communities associated with longleaf pine, but little is known about its effects on coarse woody debris and associated organisms. We conducted a 5-year study on the Osceola National Forest in northeastern Florida to determine how dormant-season prescribed burns at different frequencies (annual, biennial, quadrennial or unburned) applied over a 40-year period affected coarse woody debris volume, decomposition and nitrogen content, and subterranean termite (Reticulitermes spp.) activity. Burn frequency had no effect on standing dead tree or log volumes. However, freshly cut longleaf pine logs placed in the plots for four years lost significantly less mass in annually burned plots than in unburned plots. The annual exponential decay coefficient estimate from all logs was 0.14 yr(-1) (SE = 0.01), with the estimated times for 50 and 95% loss being 5 and 21.4 years, respectively. Termite presence was unaffected by frequent burning, suggesting they were able to survive the fires underground or within wood, and that winter burning did not deplete their food resources. C1 [Hanula, James L.; Wade, Dale D.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. [Ulyshen, Michael D.] US Forest Serv, USDA, So Res Stn, Starkville, MS 39759 USA. RP Hanula, JL (reprint author), US Forest Serv, USDA, So Res Stn, 320 Green St, Athens, GA 30602 USA. EM jhanula@fs.fed.us; mulyshen@fs.fed.us; pyrowade@gmail.com FU Nature Conservancy's Ecosystem Research Program; A.W. Mellon Foundation FX We thank A. Phalo for collecting and processing wood samples, and S. Horn, M. Cody and D. Dyer for help in sampling coarse woody debris. The Nature Conservancy's Ecosystem Research Program and the A.W. Mellon Foundation provided support for this work. We also thank three anonymous reviewers for comments that greatly improved the manuscript. NR 51 TC 3 Z9 3 U1 17 U2 64 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2012 VL 3 IS 2 BP 317 EP 331 DI 10.3390/f3020317 PG 15 WC Forestry SC Forestry GA 096OU UT WOS:000315414900010 ER PT J AU Pingree, MRA Homann, PS Morrissette, B Darbyshire, R AF Pingree, Melissa R. A. Homann, Peter S. Morrissette, Brett Darbyshire, Robyn TI Long and Short-Term Effects of Fire on Soil Charcoal of a Conifer Forest in Southwest Oregon SO FORESTS LA English DT Article DE Biscuit Wildfire; black carbon; charcoal; wildfire; peroxide-acid digestion; pre-and post-fire measurement; soil carbon; soil change ID SCOTS PINE FORESTS; BLACK CARBON; ORGANIC-MATTER; WILDFIRE; NITRIFICATION; SEDIMENTS; TEXTURE; REGIMES; STORAGE; LOSSES AB In 2002, the Biscuit Wildfire burned a portion of the previously established, replicated conifer unthinned and thinned experimental units of the Siskiyou Long-Term Ecosystem Productivity (LTEP) experiment, southwest Oregon. Charcoal C in pre and post-fire O horizon and mineral soil was quantified by physical separation and a peroxide-acid digestion method. The abrupt, short-term fire event caused O horizon charcoal C to increase by a factor of ten to >200 kg C ha(-1). The thinned wildfire treatment produced less charcoal C than unthinned wildfire and thinned prescribed fire treatments. The charcoal formation rate was 1 to 8% of woody fuels consumed, and this percentage was negatively related to woody fuels consumed, resulting in less charcoal formation with greater fire severity. Charcoal C averaged 2000 kg ha(-1) in 0-3 cm mineral soil and may have decreased as a result of fire, coincident with convective or erosive loss of mineral soil. Charcoal C in 3-15 cm mineral soil was stable at 5500 kg C ha(-1). Long-term soil C sequestration in the Siskiyou LTEP soils is greatly influenced by the contribution of charcoal C, which makes up 20% of mineral soil organic C. This research reiterates the importance of fire to soil C in a southwestern Oregon coniferous forest ecosystem. C1 [Pingree, Melissa R. A.; Homann, Peter S.] Western Washington Univ, Huxley Coll Environm, Bellingham, WA 98225 USA. [Morrissette, Brett] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Darbyshire, Robyn] US Forest Serv, USDA, Baker City, OR 97814 USA. RP Pingree, MRA (reprint author), Western Washington Univ, Huxley Coll Environm, Bellingham, WA 98225 USA. EM m.r.a.pingree@gmail.com; peter.homann@wwu.edu; brett.morrissette@oregonstate.edu; rdarbyshire@fs.fed.us FU Fund for the Enhancement of Graduate Research; Western Washington University Vice-Provost for Research; Huxley College Small Grants for Graduate Research; Biogeochemical Initiative; Long-term Ecosystem Productivity Study; USDA Pacific-Northwest Research Station, Corvallis, OR; USDA/USDI Joint Fire Science Program [10-1-10-18] FX This study was funded partially by the Fund for the Enhancement of Graduate Research awarded by the Western Washington University Vice-Provost for Research, the Huxley College Small Grants for Graduate Research and the aid given by the Biogeochemical Initiative. This work was also supported by the Long-term Ecosystem Productivity Study, USDA Pacific-Northwest Research Station, Corvallis, OR and USDA/USDI Joint Fire Science Program, Grant 10-1-10-18. We would like to thank Sara De Sitter and Ben Soderquist for removing charcoal from forest floor samples. We would like to thank Bernard Bormann for providing access to soil samples and data. NR 46 TC 9 Z9 9 U1 2 U2 31 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD JUN PY 2012 VL 3 IS 2 BP 353 EP 369 DI 10.3390/f3020353 PG 17 WC Forestry SC Forestry GA 096OU UT WOS:000315414900012 ER PT J AU Liu, LS Kost, J Yan, F Spiro, RC AF Liu, Lin Shu Kost, Joseph Yan, Fang Spiro, Robert C. TI Hydrogels from Biopolymer Hybrid for Biomedical, Food, and Functional Food Applications SO POLYMERS LA English DT Review DE hydrogel; biopolymer; hybrid; controlled drug delivery; tissue engineering; functional food ID ALGINATE HYDROGELS; IN-VITRO; BONE REGENERATION; HYALURONIC-ACID; DRUG-DELIVERY; CROSS-LINKING; PECTIN; GROWTH; PROTEIN; TISSUE AB Hybrid hydrogels from biopolymers have been applied for various indications across a wide range of biomedical, pharmaceutical, and functional food industries. In particular, hybrid hydrogels synthesized from two biopolymers have attracted increasing attention. The inclusion of a second biopolymer strengthens the stability of resultant hydrogels and enriches its functionalities by bringing in new functional groups or optimizing the micro-environmental conditions for certain biological and biochemical processes. This article presents approaches that have been used by our groups to synthesize biopolymer hybrid hydrogels for effective uses for immunotherapy, tissue regeneration, food and functional food applications. The research has achieved some challenging results, such as stabilizing physical structure, increasing mucoadhesiveness, and the creation of an artificial extracellular matrix to aid in guiding tissue differentiation. C1 [Liu, Lin Shu] USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Kost, Joseph] Ben Gurion Univ Negev, IL-84105 Beer Sheva, Israel. [Yan, Fang] Vanderbilt Univ, Sch Med, Nashville, TN 37232 USA. [Spiro, Robert C.] RCS Consulting, Half Moon Bay, CA 94019 USA. RP Liu, LS (reprint author), USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM linshu.liu@ars.usda.gov; kost@exchange.bgu.ac.il; fang.yan@vanderbilt.edu; spiro@sbcglobal.net NR 61 TC 21 Z9 21 U1 9 U2 98 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4360 J9 POLYMERS-BASEL JI Polymers PD JUN PY 2012 VL 4 IS 2 BP 997 EP 1011 DI 10.3390/polym4020997 PG 15 WC Polymer Science SC Polymer Science GA 068HH UT WOS:000313356500005 ER PT J AU Cheng, HN Gu, QM AF Cheng, H. N. Gu, Qu-Ming TI Enzyme-Catalyzed Modifications of Polysaccharides and Poly(ethylene glycol) SO POLYMERS LA English DT Review DE biocatalysis; carbohydrates; cellulosic derivatives; enzyme; guar; modification; pectin; poly(ethylene glycol); polysaccharides; starch ID SUGAR-BEET PECTIN; GUAR GALACTOMANNAN; INDUSTRIAL APPLICATIONS; ALPHA-GALACTOSIDASE; DIRECTED EVOLUTION; ASPERGILLUS-NIGER; STARCH; OXIDATION; LIPASE; HYDROLYSIS AB Polysaccharides are used extensively in various industrial applications, such as food, adhesives, coatings, construction, paper, pharmaceuticals, and personal care. Many polysaccharide structures need to be modified in order to improve their end-use properties; this is mostly done through chemical reactions. In the past 20 years many enzyme-catalyzed modifications have been developed to supplement chemical derivatization methods. Typical reactions include enzymatic oxidation, ester formation, amidation, glycosylation, and molecular weight reduction. These reactions are reviewed in this paper, with emphasis placed on the work done by the authors. The polymers covered in this review include cellulosic derivatives, starch, guar, pectin, and poly(ethylene glycol). C1 [Cheng, H. N.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. [Gu, Qu-Ming] Ashland Res Ctr, Wilmington, DE 19808 USA. RP Cheng, HN (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM hn.cheng@ars.usda.gov; qgu@ashland.com NR 96 TC 12 Z9 12 U1 10 U2 81 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2073-4360 J9 POLYMERS-BASEL JI Polymers PD JUN PY 2012 VL 4 IS 2 BP 1311 EP 1330 DI 10.3390/polym4021311 PG 20 WC Polymer Science SC Polymer Science GA 068HH UT WOS:000313356500023 ER PT J AU Toms, JD Eggert, LS Arendt, WJ Faaborg, J AF Toms, Judith D. Eggert, Lori S. Arendt, Wayne J. Faaborg, John TI A genetic polymorphism in the sex-linked ATP5A1 gene is associated with individual fitness in Ovenbirds (Seiurus aurocapilla) SO ECOLOGY AND EVOLUTION LA English DT Article DE ATP synthase; body condition; CHD1; heteroduplex molecules; migratory connectivity; survival ID Z-CHROMOSOME; MIGRATORY CONNECTIVITY; BODY CONDITION; AVIAN CHD; BIRDS; REARRANGEMENTS; SEQUENCES; WARBLERS; CHICKEN AB While testing genetic sexing techniques in Ovenbirds (Seiurus aurocapilla), we found a genetic polymorphism in the ATP5A1 gene in 38% of individuals. The Z' allele included changes in both intronic and exonic portions of the sequenced region, but there was no evidence that this changed the resulting ATP synthase product. Males that had one or more copies of this allele had higher relative bodymass (mass corrected for size) than other genotypes. This allele was unrelated to stable isotope signatures, and so was not a useful predictor of latitude within the eastern portion of the Ovenbird breeding range. Future studies are needed to determine whether this polymorphism may be a useful geographic marker. This study is the first to link polymorphisms in the sex-linked ATP5A1 gene with fitness effects. C1 [Toms, Judith D.; Eggert, Lori S.; Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Arendt, Wayne J.] US Forest Serv, Int Inst Trop Forestry, Sabana Field Res Stn, Luquillo, PR 00773 USA. RP Toms, JD (reprint author), Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. EM jdthv6@mail.missouri.edu OI Toms, Judith/0000-0002-8492-3384 FU U.S. Forest Service's International Institute of Tropical Forestry; University of Missouri FX Field sampling was funded by cooperative grants to the University of Missouri from the U.S. Forest Service's International Institute of Tropical Forestry. Lab work was conducted using new investigator funds provided to L. S. E. by the University of Missouri. NR 37 TC 3 Z9 3 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD JUN PY 2012 VL 2 IS 6 BP 1312 EP 1318 DI 10.1002/ece3.272 PG 7 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA 055WF UT WOS:000312447900018 PM 22833803 ER PT J AU Klindworth, DL Niu, ZX Chao, SM Friesen, TL Jin, Y Faris, JD Cai, XW Xu, SS AF Klindworth, Daryl L. Niu, Zhixia Chao, Shiaoman Friesen, Timothy L. Jin, Yue Faris, Justin D. Cai, Xiwen Xu, Steven S. TI Introgression and Characterization of a Goatgrass Gene for a High Level of Resistance to Ug99 Stem Rust in Tetraploid Wheat SO G3-GENES GENOMES GENETICS LA English DT Article DE wheat; Ug99; Sr47; Aegilops speltoides; chromosome engineering ID F-SP TRITICI; DURUM-WHEAT; HOMOEOLOGOUS RECOMBINATION; CONFERRING RESISTANCE; COMMON WHEAT; CYTOGENETIC CHARACTERIZATION; LOPHOPYRUM-ELONGATUM; CHROMOSOME SEGMENTS; CROP IMPROVEMENT; AESTIVUM L. AB The transfer of alien genes to crop plants using chromosome engineering has been attempted infrequently in tetraploid durum wheat (Triticum turgidum L. subsp. durum). Here, we report a highly efficient approach for the transfer of two genes conferring resistance to stem rust race Pgt-TTKSK (Ug99) from goatgrass (Aegilops speltoides) to tetraploid wheat. The durum line DAS15, carrying the stem rust resistance gene Sr47 derived from Ae. speltoides, was crossed, and backcrossed, to durum 5D(5B) aneuploids to induce homeologous pairing. After a final cross to 'Rusty' durum, allosyndetic recombinants were recovered. The Ae. speltoides chromosomal segment carrying Sr47 was found to have two stem rust resistance genes. One gene conditioning an infection type (IT) 2 was located in the same chromosomal region of 2BS as Sr39 and was assigned the temporary gene symbol SrAes7t. Based on ITs observed on a diverse set of rust races, SrAes7t may be the same as Sr39. The second gene conditioned an IT 0; and was located on chromosome arm 2BL. This gene retained the symbol Sr47 because it had a different IT and map location from other stem rust resistance genes derived from Ae. speltoides. Allosyndetic recombinant lines carrying each gene on minimal alien chromosomal segments were identified as were molecular markers distinguishing each alien segment. This study demonstrated that chromosome engineering of Ae. speltoides segments is feasible in tetraploid wheat. The Sr47 gene confers high-level and broad spectrum resistance to stem rust and should be very useful in efforts to control TTKSK. C1 [Klindworth, Daryl L.; Niu, Zhixia; Chao, Shiaoman; Friesen, Timothy L.; Faris, Justin D.; Xu, Steven S.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Cai, Xiwen] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. [Jin, Yue] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. RP Xu, SS (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM steven.xu@ars.usda.gov FU Bill & Melinda Gates Foundation; USDA-ARS CRIS [5442-22000-033-00D] FX We thank Lili Qi and Maricelis Acevedo for critically reviewing the manuscript; Mary Osenga, Danielle Holmes, and Guotai Yu for technical support; and Terry Peterson for his suggestion of Xedm80 in Ph1 multiplex tests. This research was supported in part by funds to S.S.X. provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project and the USDA-ARS CRIS Project No. 5442-22000-033-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 27 Z9 28 U1 0 U2 15 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 2160-1836 J9 G3-GENES GENOM GENET JI G3-Genes Genomes Genet. PD JUN 1 PY 2012 VL 2 IS 6 BP 665 EP 673 DI 10.1534/g3.112.002386 PG 9 WC Genetics & Heredity SC Genetics & Heredity GA 055YP UT WOS:000312454300005 PM 22690376 ER PT J AU Loesch, CR Reynolds, RE Hansen, LT AF Loesch, Charles R. Reynolds, Ronald E. Hansen, LeRoy T. TI An Assessment of Re-Directing Breeding Waterfowl Conservation Relative to Predictions of Climate Change SO JOURNAL OF FISH AND WILDLIFE MANAGEMENT LA English DT Article DE Prairie Pothole Region; economic assessment; waterfowl; climate change; breeding pairs; hatchlings ID PRAIRIE POTHOLE REGION; NORTH-DAKOTA; LAND-USE; WETLANDS; SURVIVAL; IMPACT AB The U.S. Fish and Wildlife Service has a long history of habitat conservation in the Prairie Pothole Region (PPR) of the United States that has focused on migratory birds, particularly waterfowl. The ongoing acquisition program of the U. S. Fish and Wildlife Service National Wildlife Refuge System has conserved approximately 1.1 million hectares of critical breeding waterfowl habitat. Results of recent predicted future climate scenarios are being used to suggest that waterfowl conservation be shifted away from currently important areas in the western and central portions of the U. S. PPR eastward, to locations where wetland and climate models suggest may become more conducive for providing wetland habitat for breeding ducks in the future. We used 24 years of breeding waterfowl and wetland monitoring data collected by the U. S. Fish and Wildlife Service National Wildlife Refuge System in the PPR of North and South Dakota and northeast Montana, along with land value and restoration cost data to conduct an economic assessment of the biological risk of refocusing waterfowl conservation efforts eastward due to recent projections of climate change. We considered the immediate risk of the loss of existing wetland and grassland resources in the western portion of the U. S. PPR, their current carrying capacity and production potential, the financial cost of protection vs. restoration relative to current conservation priorities, and the uncertainty of climate change effects on waterfowl habitat distribution. Because unprotected wetland and grassland habitats exist in the western and central portions of the PPR that are important for maintaining current waterfowl carrying capacity and productivity, and climate change effects are highly uncertain, maintaining the current focus of habitat protection appears to be the most cost effective approach for waterfowl habitat conservation efforts. Additionally, continued intensive monitoring activities designed to detect changing waterfowl populations and upland and wetland habitat as they relate to anthropogenic impacts (e. g., pattern tile drainage, grassland conversion) and climatic changes (e. g., wetland hydro-period), should provide more precise results to inform and adapt management and conservation activities accordingly should spatial and temporal changes in wet-dry cycles occur in the future. C1 [Loesch, Charles R.; Reynolds, Ronald E.] US Fish & Wildlife Serv, Habitat & Populat Evaluat Team, Bismarck, ND 58501 USA. [Hansen, LeRoy T.] USDA, Econ Res Serv, Washington, DC 20024 USA. RP Loesch, CR (reprint author), US Fish & Wildlife Serv, Habitat & Populat Evaluat Team, Reg 6,3425 Miriam Ave, Bismarck, ND 58501 USA. EM chuck_loesch@fws.gov OI Hansen, LeRoy/0000-0001-9038-2142 NR 48 TC 9 Z9 12 U1 2 U2 31 PU U S FISH & WILDLIFE SERVICE PI SHEPHERDSTOWN PA NATL CONSERVATION TRAINING CENTER, CONSERVATION LIBRARY, 698 CONSERVATION WAY, SHEPHERDSTOWN, WV 25443 USA SN 1944-687X J9 J FISH WILDL MANAG JI J. Fish Wildl. Manag. PD JUN PY 2012 VL 3 IS 1 BP 1 EP 22 DI 10.3996/032011-JFWM-020 PG 22 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 044NF UT WOS:000311625900001 ER PT J AU Moyle, JR Yoho, DE Whipple, SM Donoghue, AM Bramwell, RK AF Moyle, J. R. Yoho, D. E. Whipple, S. M. Donoghue, A. M. Bramwell, R. K. TI Mating behavior and fertility of broiler breeder males reared on shortened growth cycles SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE broiler breeder; fertility; light stimulation; mating behavior ID MALE DOMESTIC-FOWL; COMMERCIAL BROILER; FEED-RESTRICTION; FOOD RESTRICTION; FEMALES; WELFARE; TEMPERATURE; CHOICE; MATE AB One of the more difficult tasks when raising broiler breeder cockerels is controlling BW gain in the rearing house without inflicting excess stress. This is a period of time for the young male when many portions of the reproductive system are in the formative stages and, if neglected, can have lifelong effects on their reproductive performance. The objective of this study was to raise males under feed management programs that produced the recommended target BW of 3,060 g for males at 12, 15, 18, 21, and 24 wk of age. Males were placed at 3-wk intervals so that all males with the same BW were light stimulated at the same time but at different ages. All males were reared in the same light-controlled house at the University of Arkansas Research Farm. Males were light stimulated, and testes development, complete semen analysis, fertility, and mating activity and behavior were recorded for each group of males. At the time of light stimulation, the younger males were less successful at completing matings, crowed less often, and showed less wing flapping and neck flaring than older males, based on the results for mating behavior. Males that were older at the time of light stimulation had higher fertility than younger males. Males that were 24 wk old at the time of light stimulation had an overall fertility of 94.5%, compared with 91.8, 91.6, 79.2, and 87.3%, respectively, for the other ages. In this study, we found that males that were exposed to light at earlier ages exhibited less overall mating activity than older males. C1 [Moyle, J. R.; Yoho, D. E.; Whipple, S. M.; Bramwell, R. K.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Donoghue, A. M.] Univ Arkansas, Poultry Prod & Prod Safety Res Unit, USDA, ARS,Poultry Sci Ctr, Fayetteville, AR 72701 USA. RP Moyle, JR (reprint author), Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. EM Jon.Moyle@ARS.USDA.GOV FU US Poultry & Egg Association Harold E. Ford Foundation (Tucker, GA) [F021] FX This work was funded in by the US Poultry & Egg Association Harold E. Ford Foundation (Tucker, GA; RE Project F021). Additionally, the authors thank Janette Washington and the rest of the staff at University of Arkansas Research Farm for their assistance in conducting this research. NR 22 TC 0 Z9 0 U1 1 U2 11 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 1056-6171 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD JUN PY 2012 VL 21 IS 2 BP 272 EP 278 DI 10.3382/japr.2011-00364 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 029QS UT WOS:000310511200009 ER PT J AU Anderson, TJ Stelzer, RS Drecktrah, HG Eggert, SL AF Anderson, Timothy J. Stelzer, Robert S. Drecktrah, H. Gene Eggert, Susan L. TI Secondary production of Chironomidae in a large eutrophic lake: implications for lake sturgeon production SO FRESHWATER SCIENCE LA English DT Article DE Lake Winnebago; instantaneous growth rate; chironomid; lake sturgeon; benthos; lentic production ID POPULATION-DYNAMICS; PLUMOSUS DIPTERA; TROPHIC MODEL; WINNEBAGO WISCONSIN; LARVAL CHIRONOMIDAE; SHALLOW LAKES; GROWTH-RATES; ENERGY-FLOW; NEW-ZEALAND; RESERVOIR AB Most foodweb research in lentic systems has focused on pelagic primary (phytoplankton) and secondary (zooplankton) production as the primary energy sources for higher trophic-level production. Recent research has demonstrated that secondary production of benthic primary consumers can affect pelagic fish production and foodweb structure in lakes. We used the instantaneous growth method to calculate secondary production of chironomids in Lake Winnebago, Wisconsin (USA), where previous research has shown that lake sturgeon (Acipenser fulvescens) rely heavily on the benthos (chironomids) as a food source. We also used literature-derived data and the instantaneous growth method to calculate annual production of lake sturgeon to test whether chironomid production is sufficient to support the current lake sturgeon population in Lake Winnebago. We collected benthic samples with an Ekman grab at 4 profundal sites during ice-free conditions on 11 dates from spring 2008 through spring 2009. We measured instantaneous growth rates for 7 chironomid length classes at 5 thermal regimes in the laboratory. Mean annual density of Chironomidae was 2714 individuals/m(2), mean biomass was 2.75 g dry mass (DM)/m(2), and mean annual production of Chironomidae was 7.59 g DM m(-2) y(-1). Estimated annual production of lake sturgeon was 0.02 g DM m(-2) y(-1) in 2007. We concluded that in 2008-2009 chironomid secondary production was sufficient to support the lake sturgeon population in Lake Winnebago. The annual production rates for chironomids in Lake Winnebago are higher than rates in many other lakes in North America, presumably because of the eutrophic condition of Lake Winnebago. C1 [Anderson, Timothy J.; Stelzer, Robert S.; Drecktrah, H. Gene] Univ Wisconsin, Dept Biol & Microbiol, Oshkosh, WI 54901 USA. [Eggert, Susan L.] USDA, Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. RP Stelzer, RS (reprint author), Univ Wisconsin, Dept Biol & Microbiol, 800 Algoma Blvd, Oshkosh, WI 54901 USA. EM anderson.timothy.tja@gmail.com; stelzer@uwosh.edu; drecktra@uwosh.edu; seggert@fs.fed.us FU Wisconsin Department of Natural Resources; Graduate Student-Faculty Collaborative Research Program at the University of Wisconsin Oshkosh FX Our study was funded by the Wisconsin Department of Natural Resources and by a grant from the Graduate Student-Faculty Collaborative Research Program at the University of Wisconsin Oshkosh. We thank staff at the Wisconsin Department of Natural Resources, including R. Bruch, K. Kamke, S. Koehnke, and R. Olynyk for help with sampling on Lake Winnebago. The manuscript was greatly improved by comments from M. Lizotte, S. Koehnke, R. Koenigs, M. Butler, and 2 anonymous referees. NR 68 TC 3 Z9 4 U1 2 U2 30 PU SOC FRESWATER SCIENCE PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 2161-9565 J9 FRESHW SCI JI Freshw. Sci. PD JUN PY 2012 VL 31 IS 2 BP 365 EP 378 DI 10.1899/11-042.1 PG 14 WC Ecology; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA 021SD UT WOS:000309904800007 ER PT J AU Young, JK Hudgens, B Garcelon, DK AF Young, Julie K. Hudgens, Brian Garcelon, David K. TI Estimates of Energy and Prey Requirements of Wolverines SO NORTHWEST SCIENCE LA English DT Article DE energetics; Gulo gulo; prey requirements; wolverine ID FISHER MARTES-PENNANTI; FIELD METABOLIC-RATE; GULO-GULO; SEXUAL DIMORPHISM; UNITED-STATES; BRITISH-COLUMBIA; AMERICAN MARTEN; SOUTH-AFRICA; FOOD-HABITS; BODY-SIZE AB Wolverine (Gulo gulo) populations have decreased throughout much of their North American range and there is interest in establishing recovery programs in the Sierra Nevada of California and the Rocky Mountains of Colorado. Determining the sufficiency of prey resources is an important consideration for initiating wolverine recovery, yet there are limited data on resource availability and needs. Our goal is to estimate prey requirements based on wolverine caloric needs and the caloric content of prey likely to be available. We achieve this goal by modifying existing models to account for wolverine biology. Models show a male wolverine requires 5096 kJ/day (2925-7462 kJ) and a female wolverine requires 3645 kJ/day (2158-5439 kJ). This translates to an annual energy budget for males of 1.9 million kJ/yr that could be met by consuming the equivalent of approximately 8 mule deer/yr (Odocoileus hemionus) and 1.4 million kJ/yr for females that could be met by consuming the equivalent of less than 6 mule deer/yr. In light of published records of prey availability, these results suggest populations of wolverines could be sustained where recovery programs are being considered in Colorado and California. We suggest incorporating energetic needs of focal species, such as those calculated here for wolverines, into the assessment of resource availability before implementing recovery programs. Further, these estimates can be applied to management and conservation of wolverines throughout their range. C1 [Young, Julie K.; Hudgens, Brian; Garcelon, David K.] Inst Wildlife Studies, Arcata, CA 95518 USA. RP Young, JK (reprint author), Utah State Univ, USDA, Wildlife Serv, Natl Wildlife Res Ctr,Predator Res Stn, BNR 163, Logan, UT 84322 USA. EM julie.k.young@aphis.usda.gov FU Institute for Wildlife Studies FX We are grateful to J. Copeland for conceptual discussions. R. Powell, H. Weaver, and two anonymous Northwest Science reviewers provided editorial comments that improved the manuscript. The study was funded by the Institute for Wildlife Studies. NR 69 TC 0 Z9 0 U1 5 U2 54 PU NORTHWEST SCIENTIFIC ASSOC PI SEATTLE PA JEFFREY DUDA, USGS, WESTERN FISHERIES RES CTR, 6505 NE 65 ST, SEATTLE, WA 98115 USA SN 0029-344X EI 2161-9859 J9 NORTHWEST SCI JI Northwest Sci. PD SUM PY 2012 VL 86 IS 3 BP 221 EP 229 PG 9 WC Ecology SC Environmental Sciences & Ecology GA 999UT UT WOS:000308341200007 ER PT J AU Vogt, DF Hopey, ME Mayfield, GR Soehren, EC Lewis, LM Trent, JA Rush, SA AF Vogt, David F. Hopey, Mark E. Mayfield, G. Rad, III Soehren, Eric C. Lewis, Laura M. Trent, John A. Rush, Scott A. TI Stopover Site Fidelity by Tennessee Warblers at a Southern Appalachian High-elevation Site SO WILSON JOURNAL OF ORNITHOLOGY LA English DT Article ID MIGRATION; ICELAND; RATES AB We examined stopover site fidelity by Tennessee Warblers (Oreothlypis peregrina) at two Tennessee banding stations (Whigg Meadow and Big Bald) operated during fall migration, similar to 1,000 km from the nearest breeding areas. We captured and banded 4,324 Tennessee Warblers at Whigg Meadow from 1999 to 2008 with 14 individuals (0.3%) recaptured in subsequent years. We banded 5,514 Tennessee Warblers at Big Bald from 2003 to 2008 where, despite relatively close geographical proximity to Whigg Meadow (<150 km between sites), no individuals were recaptured outside of the initial capture year. These inter-annual recaptures, to our knowledge, reflect the highest reoccurrence of a Nearctic-neotropical migratory passerine at a single stopover site. Our results provide evidence that passerine stopover site fidelity may occur at considerable distances from both breeding and wintering areas, and differ between geographically similar stopover sites. Received 19 May 2011. Accepted 12 November 2011. C1 [Rush, Scott A.] Univ Windsor, Great Lakes Inst Environm Res, Windsor, ON N9B 3P4, Canada. [Hopey, Mark E.] So Appalachian Raptor Res, Big Bald Banding Stn, Mars Hill, NC 28754 USA. [Soehren, Eric C.; Trent, John A.] Alabama Dept Conservat & Nat Resources, Wehle Land Conservat Ctr, State Lands Div, Elhew Field Stn, Midway, AL 36053 USA. [Lewis, Laura M.] Forest Serv, USDA, Cleveland, TN 37312 USA. RP Rush, SA (reprint author), Univ Windsor, Great Lakes Inst Environm Res, Windsor, ON N9B 3P4, Canada. EM srush@uwindsor.ca FU USDA Forest Service; Cherokee National Forest; Tennessee Ornithological Society FX This research was made possible through the assistance of numerous volunteers. Funding and logistical support was provided by the USDA Forest Service, Cherokee National Forest and a Katherine A. Goodpasture Award from the Tennessee Ornithological Society. We are indebted to Scott Somershoe and two anonymous reviewers for comments provided on earlier drafts of this manuscript. NR 20 TC 4 Z9 4 U1 3 U2 10 PU WILSON ORNITHOLOGICAL SOC PI WACO PA 5400 BOSQUE BLVD, STE 680, WACO, TX 76710 USA SN 1559-4491 J9 WILSON J ORNITHOL JI Wilson J. Ornithol. PD JUN PY 2012 VL 124 IS 2 BP 366 EP 370 PG 5 WC Ornithology SC Zoology GA 005YO UT WOS:000308786200021 ER PT J AU McDonald, LW Goheen, SC Donald, PA Campbell, JA AF McDonald, L. W. Goheen, S. C. Donald, P. A. Campbell, J. A. TI IDENTIFICATION AND QUANTITATION OF VARIOUS INOSITOLS AND O-METHYLINOSITOLS PRESENT IN PLANT ROOTS RELATED TO SOYBEAN CYST NEMATODE HOST STATUS SO NEMATROPICA LA English DT Article DE Inositol; O-methyl-inositol; Gas chromatography/mass spectrometry; Plant roots ID GAS-LIQUID-CHROMATOGRAPHY; DROUGHT TOLERANCE; PINITOL; ONONITOL; DERIVATIZATION; ACCUMULATION; SPECTROMETRY; LEGUMES AB Inositols and O-methylinositols in plant roots were extracted, isolated, and analyzed to test the hypothesis that these compounds may be involved in feeding site establishment for soybean cyst nematode. Root samples from soybean (Glycine max), tapia bean (Phaseolus vulgaris), crimson clover (Trifolium incarnatum), corn (Zea mays), sugar beet (Beta vulgaris), and peanut (Arachis hypogaea) were extracted with 80% ethanol, derivatized, and analyzed using gas chromatography/mass spectrometry (GC/MS). Fragment ions at m/z 260 and 265 were used to quantify the O-methylinositols and inositols, respectively. Pinitol, D-chiro-inositol, and myo-inositol were found in soybean samples and myo-inositol in tapia bean. Sugar beets contained D-chiro-inositol. Pinitol and myo-inositol were found in crimson clover. Corn contained myo-inositol. Pinitol, D-chiro-inositol, ononitol, and myo-inositol were found in peanuts. No correlation was found between the presence of any of the inositols or o-methyl inositols and the ability of these plants to host the soybean cyst nematode. C1 [Goheen, S. C.; Campbell, J. A.] Pacific NW Natl Lab, Richland, WA 99352 USA. [McDonald, L. W.] Washington State Univ, Dept Chem, Pullman, WA 99164 USA. [Donald, P. A.] USDA ARS, Crop Genet Res Unit, Jackson, TN 38301 USA. RP Campbell, JA (reprint author), Pacific NW Natl Lab, Richland, WA 99352 USA. EM James.Campbell@pnnl.gov FU Pacific Northwest National Laboratory [DE-AC05-76RLO 1830] FX We thank Amy Zellman for reviewing this manuscript and the U.S. Department of Energy, Office of Science for providing the Science Undergraduate Laboratory Internship (SULI). This research was conducted at Pacific Northwest National Laboratory, operated by Battelle for the U.S. Department of Energy under contract No. DE-AC05-76RLO 1830. We also extend special thanks to Dr. Laurens Anderson for his support and advice. NR 24 TC 0 Z9 0 U1 5 U2 9 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 J9 NEMATROPICA JI Nematropica PD JUN PY 2012 VL 42 IS 1 BP 1 EP 8 PG 8 WC Zoology SC Zoology GA 995FE UT WOS:000307992500003 ER PT J AU Cabos, RYM Hara, AH Tsang, MMC AF Cabos, R. Y. M. Hara, A. H. Tsang, M. M. C. TI HOT WATER DRENCH TREATMENT FOR CONTROL OF RENIFORM NEMATODES IN POTTED DRACAENA SO NEMATROPICA LA English DT Article DE Dracaena; Drenching; Hot water; Potting media; Reniform nematodes; Rotylenchulus reniformis ID RADOPHOLUS-SIMILIS; BURROWING NEMATODE; DISINFESTATION; IMMERSION; HOMOPTERA; PLANTS; PALMS AB A continuous hot water drench treatment was evaluated for disinfesting potted dracaena of reniform nematodes, Rotylenchulus reniformis. Modifications were made to a hot water shower container to allow the delivery of a continuous stream of hot water directly to the media and roots of infested plants. Reniform nematodes were successfully eliminated in dracaena of marketable age treated at 50 degrees C for 10 minutes or longer. No evidence of thermal damage was observed on plants drenched with hot water even at 52 degrees C for 14 minutes. Continuous drenching for 15 minutes at 50 degrees C is recommended to ensure effective penetration of water through the media. C1 [Cabos, R. Y. M.] Agr Res Serv, USDA, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Hara, A. H.] Univ Hawaii Manoa, Coll Trop Agr & Human Resources, Hilo, HI 96720 USA. [Tsang, M. M. C.] Univ Hawaii, Coll Agr Forestry & Nat Resource Management, Hilo, HI 96720 USA. RP Cabos, RYM (reprint author), Agr Res Serv, USDA, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM roxana.cabos@ars.usda.gov FU U.S. Department of Agriculture (USDA) Special Floriculture Grant [2010-34199-21332] FX The authors wish to thank Cathy Mello, Susan Cabral, Jon Katada, Kris Aoki, Noel Nakamura, Jade Miyashiro, and Ruth Niino-DuPonte for their assistance in running the trials and to California-Hawaii Foliage Growers for providing the plants used in this study. This research was funded in part by the U.S. Department of Agriculture (USDA) Special Floriculture Grant (2010-34199-21332). NR 19 TC 1 Z9 1 U1 1 U2 5 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 J9 NEMATROPICA JI Nematropica PD JUN PY 2012 VL 42 IS 1 BP 72 EP 79 PG 8 WC Zoology SC Zoology GA 995FE UT WOS:000307992500012 ER PT J AU Donald, PA Heinz, R Bernard, E Hershman, D Hensley, D Flint-Garcia, S Joost, R AF Donald, P. A. Heinz, R. Bernard, E. Hershman, D. Hensley, D. Flint-Garcia, S. Joost, R. TI DISTRIBUTION, HOST STATUS AND POTENTIAL SOURCES OF RESISTANCE TO VITTATIDERA ZEAPHILA SO NEMATROPICA LA English DT Article DE goosegrass; Eleusine indica; Zea mays; teosinte; native grasses; switchgrass ID MAIZE AB Vittatidera zeaphila was described from stunted Zea mays (corn) roots collected in northwestern Tennessee (Obion County) in 2006. Similar cyst specimens had previously been collected in 1978 from Lauderdale County, TN, on Eleusine indica (goosegrass). Comparison of the 1978 specimens deposited in the USDA Nematology Collection at Beltsville, MD, and the 2006 specimens verified that they were identical. The purpose of this research was to determine the distribution of this nematode, determine the host range and determine if there was resistance which could be incorporated into commercial corn hybrids in the event the nematode was a threat to Midwest corn production. This nematode is known only from the 1978 site in Lauderdale County, TN and the two findings in Obion County, Tennessee and a field in Hickman County, Kentucky from this study. Zea mays and E. indica were the only plants supporting increase of this nematode and were good hosts, with Rf values of 6 or more. Nonhosts included common dicotyledous plants that are known hosts of cyst nematodes, a range of native grasses, and teosinte. Preliminary data on potential sources of resistance to V. zeaphila suggest that resistance is not a dominant trait and that there is a potential role of the cytoplasm in conferring resistance. C1 [Donald, P. A.] USDA ARS, Crop Genet Res Unit, Jackson, TN USA. [Heinz, R.] Univ Missouri, Soil Testing Serv, Columbia, MO USA. [Bernard, E.; Hensley, D.] Univ Tennessee, Knoxville, TN USA. [Hershman, D.] Univ Kentucky, Princeton, KY USA. [Flint-Garcia, S.] USDA ARS, Plant Genet Res Unit, Columbia, MO USA. [Joost, R.] Smith Bucklin, St Louis, MO USA. [Heinz, R.] Univ Missouri, Plant Diagnost Serv, Columbia, MO USA. RP Donald, PA (reprint author), USDA ARS, Crop Genet Res Unit, Jackson, TN USA. EM pat.donald@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service [6402-21220-009-00D] FX We thank Barbara Michaud, Gordon White, and Colleen Staggs for technical assistance. This research was partially funded by United States Department of Agriculture, Agricultural Research Service, project number 6402-21220-009-00D. NR 7 TC 0 Z9 0 U1 0 U2 6 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 J9 NEMATROPICA JI Nematropica PD JUN PY 2012 VL 42 IS 1 BP 91 EP 95 PG 5 WC Zoology SC Zoology GA 995FE UT WOS:000307992500015 ER PT J AU Falkner, M Linthicum, KJ Britch, SC Mao, L Tatem, A AF Falkner, M. Linthicum, K. J. Britch, S. C. Mao, L. Tatem, A. TI Spatio-temporal dispersion of Aedes taeniorhynchus in Florida SO INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES LA English DT Meeting Abstract C1 [Falkner, M.] Univ Floirida, Gainesville, FL USA. [Linthicum, K. J.; Britch, S. C.] USDA, Gainesville, FL USA. [Mao, L.; Tatem, A.] Univ Florida, Gainesville, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1201-9712 J9 INT J INFECT DIS JI Int. J. Infect. Dis. PD JUN PY 2012 VL 16 SU 1 BP E125 EP E125 DI 10.1016/j.ijid.2012.05.285 PG 1 WC Infectious Diseases SC Infectious Diseases GA 999YX UT WOS:000308353100270 ER PT J AU Watts, AC Ambrosia, VG Hinkley, EA AF Watts, Adam C. Ambrosia, Vincent G. Hinkley, Everett A. TI Unmanned Aircraft Systems in Remote Sensing and Scientific Research: Classification and Considerations of Use SO REMOTE SENSING LA English DT Article DE Unmanned Aircraft System (UAS); UAV; drone; aerial survey ID IMAGERY AB Unmanned Aircraft Systems (UAS) have evolved rapidly over the past decade driven primarily by military uses, and have begun finding application among civilian users for earth sensing reconnaissance and scientific data collection purposes. Among UAS, promising characteristics are long flight duration, improved mission safety, flight repeatability due to improving autopilots, and reduced operational costs when compared to manned aircraft. The potential advantages of an unmanned platform, however, depend on many factors, such as aircraft, sensor types, mission objectives, and the current UAS regulatory requirements for operations of the particular platform. The regulations concerning UAS operation are still in the early development stages and currently present significant barriers to entry for scientific users. In this article we describe a variety of platforms, as well as sensor capabilities, and identify advantages of each as relevant to the demands of users in the scientific research sector. We also briefly discuss the current state of regulations affecting UAS operations, with the purpose of informing the scientific community about this developing technology whose potential for revolutionizing natural science observations is similar to those transformations that GIS and GPS brought to the community two decades ago. C1 [Watts, Adam C.] Univ Florida, Sch Nat Resources & Environm, Gainesville, FL 32611 USA. [Ambrosia, Vincent G.] Calif State Univ, Monterey Bay NASA, Ames Res Ctr, Moffett Field, CA 94035 USA. [Hinkley, Everett A.] US Forest Serv, USDA, Arlington, VA 22209 USA. RP Watts, AC (reprint author), Univ Florida, Sch Nat Resources & Environm, POB 110410, Gainesville, FL 32611 USA. EM acwatts@ufl.edu; vincent.g.ambrosia@nasa.gov; ehinkley@fs.fed.us NR 21 TC 128 Z9 134 U1 30 U2 209 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD JUN PY 2012 VL 4 IS 6 BP 1671 EP 1692 DI 10.3390/rs4061671 PG 22 WC Remote Sensing SC Remote Sensing GA 978QB UT WOS:000306759100009 ER PT J AU Reagel, PF Smith, MT Hanks, LM AF Reagel, Peter F. Smith, Michael T. Hanks, Lawrence M. TI Effects of larval host diameter on body size, adult density, and parasitism of cerambycid beetles SO CANADIAN ENTOMOLOGIST LA English DT Article ID COLEOPTERA; PINE AB In this study, we assessed the relationship between the size of bolts cut from pin oak trees, Quercus palustris Munchhausen (Fagaceae), and eastern white pines, Pinus strobus Linnaeus (Pinaceae), and the number and body size of cerambycid beetles (Coleoptera: Cerambycidae) that develop within them. From oak bolts emerged adult Graphisurus fasciatus (De Geer) (98% of beetles) and Xylotrechus colonus (Fabricius), while pine bolts produced Monochamus carolinensis (Olivier) (95%) and Astylopsis sexguttata (Say). The number of G. fasciatus was positively correlated with the diameter of the oak bolts, while the greatest number of M. carolinensis emerged from pine bolts of intermediate diameter. Body size of both species was positively correlated with bolt diameter. Rates of parasitism were very low, only 0.9% for oaks, and averaging 5.3 +/- 8.6% across pine bolts. Oak bolts yielded the braconid wasps (Hymenoptera: Braconidae) Wroughtonia ferruginea (Brues) and a species in the genus Atanycolus Forster (similar to Atanycolus charus (Riley)), and an ichneumonid (Hymenoptera: Ichneumonidae) in the genus Demopheles Forster. Pine bolts produced a braconid in the genus Digonogastra Viereck, and the tachinid fly (Diptera: Tachinidae) Billaea monohammi (Townsend). C1 [Reagel, Peter F.; Hanks, Lawrence M.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Smith, Michael T.] ARS, USDA, Beneficial Insects Intro Res Lab, Newark, DE 19713 USA. RP Hanks, LM (reprint author), Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. EM hanks@life.illinois.edu FU Agricultural Research Service of USDA [58-1926-3-339]; Alphawood Foundation FX We thank E.E. Graham, R.F. Mitchell, A.M. Ray, M.L. Richardson, D.E. Philpott, M.C. Currier, and J.F. Hitchens for assistance in the field, and J.J. Rodriquez, A.A. Velario, J.B. Whitfield, and N.E. Woodley for help with taxonomy. We appreciate funding support from the Agricultural Research Service of USDA under agreement #58-1926-3-339, and the Alphawood Foundation. This research was in partial fulfilment of a Ph.D. degree for P.F.R. from the University of Illinois at Urbana-Champaign. NR 10 TC 2 Z9 2 U1 1 U2 14 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0008-347X J9 CAN ENTOMOL JI Can. Entomol. PD JUN PY 2012 VL 144 IS 3 BP 435 EP 438 DI 10.4039/tce.2012.39 PG 4 WC Entomology SC Entomology GA 984ZP UT WOS:000307232800005 ER PT J AU Engeman, RM Hartmann, JW Beckerman, SF Seamans, TW Abu-Absi, S AF Engeman, Richard M. Hartmann, John W. Beckerman, Scott F. Seamans, Thomas W. Abu-Absi, Sarah TI Egg Oiling to Reduce Hatch-Year Ring-Billed Gull Numbers on Chicago's Beaches During Swim Season and Water Quality Test Results SO ECOHEALTH LA English DT Article DE bacterial exceedances; E. coli contamination; Larus delawarensis; population monitoring; swim advisories; swim bans ID ESCHERICHIA-COLI; GREAT-LAKES; HERRING-GULLS; DISPERSAL; POLLUTION AB A burgeoning ring-billed gull population along Chicago's Lake Michigan beaches contributes to degraded water quality through fecal contamination. Egg oiling was conducted at Chicago's gull colonies to reduce production and the influx of hatch-year (HY) gulls using Chicago's beaches, with a second, long-term objective of eventually reducing adult gull numbers through attrition. We also investigated swim season water quality trends through the course of this work. From 2007 to 2009, 52, 80, and 81%, of nests at the two primary nest colonies had their eggs rendered inviable by corn oil application. Counts of HY and after hatch-year (AHY) gulls were analyzed during treatment years for 10 beaches. Water quality data were available from the Chicago Park District during our three treatment years and the prior year (baseline) for 19 beaches. HY counts declined at all 10 surveyed beaches from the initial year (52% nests with oiled eggs) to subsequent years with similar to 80% of nests oiled. Overall, HY gulls numbers on beaches decreased 86% from 2007 to 2009. Decreases in beach usage by AHY gulls were not detected. Compared to pretreatment, the number of beaches with improved water quality test rates increased each year through the course of the study. The frequency of water quality tests showing bacterial exceedances compared to 2006 declined at 18 of 19 beaches by 2009. Egg oiling resulted in fewer HY gulls using Chicago's beaches and was likely a beneficial factor for reduced frequencies of swim advisories and swim bans. C1 [Engeman, Richard M.] Wildlife Serv, USDA APHIS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Hartmann, John W.; Beckerman, Scott F.] Wildlife Serv, USDA APHIS, Springfield, IL 62703 USA. [Seamans, Thomas W.] Wildlife Serv, USDA APHIS, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. [Abu-Absi, Sarah] Chicago Dept Environm, WRD Environm, Chicago, IL 60602 USA. RP Engeman, RM (reprint author), Wildlife Serv, USDA APHIS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Richard.m.engeman@aphis.usda.gov NR 26 TC 3 Z9 3 U1 7 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-9202 J9 ECOHEALTH JI EcoHealth PD JUN PY 2012 VL 9 IS 2 BP 195 EP 204 DI 10.1007/s10393-012-0760-z PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 987GV UT WOS:000307406100010 PM 22492207 ER PT J AU Ayala, R Griswold, T AF Ayala, Ricardo Griswold, Terry TI Two new species of the bee genus Peponapis, with a key to the North and Central American species (Hymenoptera: Apidae: Eucerini) SO REVISTA MEXICANA DE BIODIVERSIDAD LA English DT Article DE taxonomy; Hymenoptera; Apidae; squash bees; Cucurbitaceae; Schizocarpum ID GOURD BEES; APOIDEA; SQUASH AB Two new species of squash bees, Peponapis pacifica Ayala and Griswold sp. n. and P parkeri Griswold and Ayala sp. n., are described and illustrated. Peponapis pacifica is oligolectic on flowers of Schizocarpum longisepalum (Cucurbitaceae) endemic to Mexico, where it is found in the tropical dry forest along the Pacific Coast, between Sonora and Chiapas and in the Balsas River basin; and P parkeri is known only from the Pacific slope of Costa Rica. A key for the North and Central American species of Peponapis is provided. C1 [Ayala, Ricardo] Univ Nacl Autonoma Mexico, Inst Biol, Estn Biol Chamela, San Patricio 48980, Jalisco, Mexico. [Griswold, Terry] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. RP Ayala, R (reprint author), Univ Nacl Autonoma Mexico, Inst Biol, Estn Biol Chamela, Apartado Postal 21, San Patricio 48980, Jalisco, Mexico. EM rayala@ibiologia.unam.mx NR 16 TC 0 Z9 0 U1 1 U2 5 PU INST BIOLOGIA, UNIV NACIONAL AUTONOMA MEXICO PI MEXICO PA APARTADO POSTAL 70-233, MEXICO, D F 00000, MEXICO SN 1870-3453 J9 REV MEX BIODIVERS JI Rev. Mex. Biodivers. PD JUN PY 2012 VL 83 IS 2 BP 396 EP 406 PG 11 WC Biodiversity Conservation SC Biodiversity & Conservation GA 980KL UT WOS:000306892000010 ER PT J AU Knutson, AE DeLoach, CJ Tracy, JL Randal, CW AF Knutson, Allen E. DeLoach, C. Jack Tracy, James L. Randal, Charles W. TI Field Evaluation of Diorhabda elongata and D. carinata (Coleoptera: Chrysomelidae) for Biological Control of Saltcedars (Tamarix spp.) in Northwest Texas SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID LEAF BEETLE; CONTROL AGENT; DESERTICOLA COLEOPTERA; DIFFERENT POPULATIONS; DIAPAUSE AB Four species of Diorhabda (Coleoptera: Chrysomelidae) have been approved for release as biological control agents of invasive saltcedars (Tamarix spp.) in the U.S. Two species, D. elongate (Brulle) and D. carinata (Faldermann), were evaluated for their ability to establish populations in northwest Texas (from 32.5 degrees N to 34.0 degrees N) and increase in numbers sufficient to defoliate saltcedar trees. Following releases of adults during 2 years, D. elongate overwintered, established, and increased sufficiently to defoliate saltcedar trees at all three release sites. In contrast, D. carinata overwintered in the field at only one of three sites and then only in very low numbers. D. carinata did not defoliate saltcedar trees outside of cages at any release site. After 2 years of release efforts, D. carinata could not be detected at any of the three release sites during the third year and was not considered to have established. Survival of overwintering adult D. carinata was significantly greater than survival of overwintering D. elongate adults. However, D. carinata adults terminated overwintering quiescence earlier and were observed on saltcedar trees earlier in February and March than were D. elongate adults. These results suggested that emergence of adults from overwintering quiescence in late February and early March exposed D. carinata to freezing weather and contributed to the failure of the species to establish in northwest Texas. In contrast, D. elongate terminated overwintering quiescence latter and therefore experiences less risk of exposure to spring frost. These results suggested that D. elongate will be more effective than D. carinata for biological control of saltcedar in northwest Texas (32.5 degrees to 34.0 degrees N). C1 [Knutson, Allen E.] Texas AgriLife Res & Extens Ctr, Dallas, TX 75252 USA. [DeLoach, C. Jack] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Tracy, James L.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Randal, Charles W.] USDA APHIS PPQ, Olney, TX USA. RP Knutson, AE (reprint author), Texas AgriLife Res & Extens Ctr, 17360 Coit Rd, Dallas, TX 75252 USA. EM a-knutson@tamu.edu FU USDA-Sustainable Agriculture Research and Education Program; Colorado River Municipal Water District FX This project supported in part from grants from the USDA-Sustainable Agriculture Research and Education Program and the Colorado River Municipal Water District. Thanks to Dan Bean, Colorado State Insectary, Colorado Department of Agriculture, Palisades, CO for providing adult D. carinata used in this study. Thanks also to Manuel Campos and Bryan Stokes, Texas AgriLife Extension Service, and Riley Kitchens, Natural Resources Conservation Service, for their assistance with this study and to Carlos Campos for help with statistical analysis. NR 20 TC 2 Z9 2 U1 2 U2 15 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2012 VL 37 IS 2 BP 91 EP 102 PG 12 WC Entomology SC Entomology GA 988JX UT WOS:000307487700001 ER PT J AU Burd, JD Prasifka, JR Bradshaw, JD AF Burd, John D. Prasifka, Jarrad R. Bradshaw, Jeffrey D. TI Establishment and Host Effects of Cereal Aphids on Switchgrass (Panicum virgatum L.) Cultivars SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID HOMOPTERA; HEMIPTERA; HYMENOPTERA; APHIDIIDAE; ABUNDANCE; ARMYWORM; GREENBUG; GRASSES AB Potential for widespread plantings of switchgrass, Panicum virgatum L., as a biomass feedstock prompted its evaluation as a host for economically important cereal aphids. Seedlings of four cultivars of switchgrass, 'Kanlow', 'Blackwell', 'Cave-In-Rock', and 'Sunburst', were evaluated as hosts for greenbug, Schizaphis graminum (Rondani), biotypes E, I, and Florida; Russian wheat aphid, Diuraphis noxia (Kurdj.), biotype 2; bird-cherry oat aphid, Rhopalosiphum padi (L.); corn leaf aphid, R. maidis (Fitch); English grain aphid, Sitobion avenae (F.); and yellow sugarcane aphid, Sipha flava (Forbes). Greenbug biotype E and Russian wheat aphid biotype 2, both economically important cereal aphids in the United States, and English grain aphid were unable to establish on any of the switchgrass cultivars. However, greenbug biotypes I and Florida, bird-cherry oat aphid, corn leaf aphid, and yellow sugarcane aphid established on all cultivars tested. The aphids which successfully established on switchgrass also reduced plant growth (number of leaves added and leaf biomass), usually by 50% or more. Interactions between greenbug biotypes and cultivars, along with observations of other aphids on switchgrass cultivars, suggested potential for developing cultivars resistant to specific aphids and biotypes. C1 [Burd, John D.; Prasifka, Jarrad R.; Bradshaw, Jeffrey D.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. [Burd, John D.] USDA ARS, Wheat Peanut & Other Forage Crop Res Unit, Stillwater, OK 74075 USA. RP Burd, JD (reprint author), USDA ARS, No Crop Sci Lab, 1307 18th St N, Fargo, ND 58102 USA. NR 22 TC 7 Z9 7 U1 2 U2 22 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2012 VL 37 IS 2 BP 115 EP 122 PG 8 WC Entomology SC Entomology GA 988JX UT WOS:000307487700003 ER PT J AU Jackson, RE Snodgrass, GL Allen, KC Perera, OP Price, L AF Jackson, R. E. Snodgrass, G. L. Allen, K. C. Perera, O. P. Price, L. TI Analysis of Carbon and Nitrogen Isotopes for Natal Host Determination of Tarnished Plant Bug (Hemiptera: Miridae) Adults SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID FOOD-WEB; HETEROPTERA MIRIDAE; HELICOVERPA-ZEA; GEORGES BANK; POPULATIONS; MISSISSIPPI; RESISTANCE; DELTA; ARKANSAS; COTTON AB Tarnished plant bug, Lygus lineolaris (Palisot de Beauvois), is the major insect pest of cotton, Gossypium hirsutum L., in the mid-South. This pest moves into cotton fields from non-cotton hosts during late spring and early summer. Stable carbon isotope (SCI) analysis was used to characterize plant hosts of tarnished plant bugs, as well as adult tarnished plant bugs that had developed as immatures on the plants. Plants of two host types (C-3 versus C-4) were identified using the SCI analysis, and tarnished plant bug adults reared as immatures on the plants retained a carbon isotopic signature similar to the host plant. Thus, carbon isotope ratios of tarnished plant bugs reared on C-3 plants differed from those reared on C-4 plants. Carbon and nitrogen isotope ratios were analyzed jointly using cluster and discriminant analyses to discriminate between adults that developed as immatures on two primary C-4 hosts (maize, Zea mays L., and pigweed, Amaranthus spp.). These analyses distinguished tarnished plant bugs that developed as nymphs on maize from those that developed on pigweed. To evaluate the impact on the carbon isotopic signature of immature tarnished plant bugs moving from one host type to another, nymphs were reared for the first three instars on broccoli, Brassica oleracea L. var. italica, (a C-3 plant) and transferred to maize (a C-4 plant) for the final two instars. The reverse was also done, and isotope ratios of these insects were compared to those that completed all instars on either broccoli or maize. The isotopic signature of the host plant was primarily obtained by tarnished plant bugs during the last two instars. Results from this study demonstrated the utility of carbon and nitrogen isotope ratio determination for evaluating the ecology of host plants of tarnished plant bugs in the mid-South. Knowledge of the primary hosts of tarnished plant bugs could allow for population management/reduction of these insects before moving into cotton. C1 [Jackson, R. E.; Snodgrass, G. L.; Allen, K. C.; Perera, O. P.; Price, L.] USDA ARS, So Insect Management Res Unit, Stoneville, MS 38776 USA. RP Jackson, RE (reprint author), USDA ARS, So Insect Management Res Unit, Stoneville, MS 38776 USA. NR 19 TC 1 Z9 1 U1 1 U2 11 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2012 VL 37 IS 2 BP 123 EP 132 PG 10 WC Entomology SC Entomology GA 988JX UT WOS:000307487700004 ER PT J AU Burd, JD Puterka, GJ AF Burd, John D. Puterka, Gary J. TI Occurrence of Hymenopterous Parasitoids of Diuraphis spp. in the Western Rocky Mountain Region SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID APHIDIDAE; WHEAT; KEY; APHELINIDAE; HOMOPTERA; COLORADO; RECOVERY C1 [Burd, John D.; Puterka, Gary J.] USDA ARS, Wheat Peanut & Other Forage Crop Res Unit, Stillwater, OK 74075 USA. RP Burd, JD (reprint author), USDA ARS, Wheat Peanut & Other Forage Crop Res Unit, 1301 N Western Rd, Stillwater, OK 74075 USA. NR 12 TC 0 Z9 0 U1 0 U2 2 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD JUN PY 2012 VL 37 IS 2 BP 231 EP 234 PG 4 WC Entomology SC Entomology GA 988JX UT WOS:000307487700016 ER PT J AU Liu, YB AF Liu, Yong-Biao TI Ultralow oxygen treatment for control of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae), on harvested table grapes SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Controlled atmosphere; Ultralow oxygen; Postharvest pest control; Phytosanitary; Table grape; Western flower thrips ID POSTHARVEST CONTROL; ICEBERG LETTUCE; QUALITY AB Exported organic table grapes from the U.S. are currently fumigated with methyl bromide to control quarantined pests such as western flower thrips, Frankliniella occidentalis (Pergande). An alternative treatment which is compatible with organic products is needed. In this study, controlled atmosphere with ultralow oxygen (ULO) treatment was studied to control thrips on harvested table grapes. ULO treatments with <0.01 ppm oxygen at 3.3 degrees C for 1, 2, and 3 days were tested for thrips control. The 3-day ULO treatment achieved complete control of thrips. The treatment was also applied to Thompson seedless and Flame seedless grape varieties and grape quality was evaluated two weeks after the treatment. The ULO treatment had no negative impact on the visual quality of grapes. There were no significant differences in overall grape quality or premium quality berries between the ULO treatment and the control. The results indicated that the ULO treatment was effective and safe to control western flower thrips on table grapes and had a potential for commercial use. (C) Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society, 2012. Published by Elsevier B.V. All rights reserved. C1 USDA ARS, Salinas, CA 93905 USA. RP Liu, YB (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM yongbiao.liu@ars.usda.gov FU U.S. Department of Agriculture, Foreign Agricultural Service; California Table Grape Research Commission FX I thank T. Masuda and J. Wasson for technical assistance. This study was supported in part by funding from the U.S. Department of Agriculture, Foreign Agricultural Service and the California Table Grape Research Commission. NR 14 TC 2 Z9 2 U1 2 U2 11 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD JUN PY 2012 VL 15 IS 2 BP 269 EP 271 DI 10.1016/j.aspen.2012.02.004 PG 3 WC Entomology SC Entomology GA 983AW UT WOS:000307089600013 ER PT J AU Olsen, SC Johnson, CS AF Olsen, S. C. Johnson, C. S. TI Efficacy of Dart or Booster Vaccination with Strain RB51 in Protecting Bison against Experimental Brucella abortus Challenge SO CLINICAL AND VACCINE IMMUNOLOGY LA English DT Article ID IMMUNE-RESPONSES; MUTANT STRAINS; INFECTION; CATTLE; ORIGIN; GOATS AB This study characterized the efficacy of the Brucella abortus strain RB51 vaccine in bison when delivered by single intramuscular vaccination (hand RB51), by single pneumatic dart delivery (dart RB51), or as two vaccinations approximately 13 months apart (booster RB51) in comparison to control bison. All bison were challenged intraconjunctivally in midgestation with 10(7) CFU of B. abortus strain 2308 (S2308). Bison were necropsied and sampled within 72 h of abortion or delivery of a live calf. Compared to nonvaccinated bison, bison in the booster RB51 treatment had a reduced (P < 0.05) incidence of abortion, uterine infection, or infection in maternal tissues other than the mammary gland at necropsy. Bison in single-vaccination treatment groups (hand RB51 and dart RB51) did not differ (P > 0.05) from the control group in the incidence of abortion or recovery of S2308 from uterine, mammary, fetal, or maternal tissues at necropsy. Compared to nonvaccinated animals, all RB51 vaccination groups had reduced (P < 0.05) mean colonization or incidence of infection in at least 2 of 4 target tissues, with the booster RB51 group having reduced (P < 0.05) colonization and incidence of infection in all target tissues. Our data suggest that booster vaccination of bison with RB51 enhances protective immunity against Brucella challenge compared to single vaccination with RB51 by hand or by pneumatic dart. Our study also suggests that an initial vaccination of calves followed by booster vaccination as yearlings should be an effective strategy for brucellosis control in bison. C1 [Olsen, S. C.; Johnson, C. S.] ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Olsen, SC (reprint author), ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM Steven.olsen@ars.usda.gov NR 23 TC 8 Z9 9 U1 2 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1556-6811 J9 CLIN VACCINE IMMUNOL JI Clin. Vaccine Immunol. PD JUN PY 2012 VL 19 IS 6 BP 886 EP 890 DI 10.1128/CVI.00107-12 PG 5 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA 983HN UT WOS:000307110500009 PM 22496493 ER PT J AU Kumar, KP Murugan, K Kovendan, K Kumar, AN Hwang, JS Barnard, DR AF Kumar, Kanagarajan Prasanna Murugan, Kadarkarai Kovendan, Kalimuthu Kumar, Arjunan Naresh Hwang, Jiang-Shiou Barnard, Donald R. TI Combined effect of seaweed (Sargassum wightii) and Bacillus thuringiensis var. israelensis on the coastal mosquito, Anopheles sundaicus, in Tamil Nadu, India SO SCIENCEASIA LA English DT Article DE larvicidal activity; repellency; vector control; malarial vector ID CULEX-QUINQUEFASCIATUS SAY; INSECT/MOSQUITO REPELLENT PLANTS; VECTOR MOSQUITOS; STEPHENSI LISTON; MALARIAL VECTOR; LEAF EXTRACTS; ESSENTIAL OIL; RED ALGA; CULICIDAE; DIPTERA AB Laboratory studies were made to determine the effectiveness of seaweed (Sargassum wightii) extract combined with Bacillus thuringiensis var. israelensis for the control of Anopheles sundaicus Liston, a malaria vector that occurs in the coastal areas of peninsular India. The results revealed that the different concentrations of crude extract of S. wightii resulted in considerable mortality and the LC50 value for 1 Instar larvae at 1.0 mg/l was 0.88, for II Instar 0.73, for III Instar 1.34, for IV Instar 1.56, and for pupa 1.71. The LC90 values of T, 11, III, and IV Instar, and pupa were 2.73, 2.43, 3.03, 3.21, and 3.23 mg/ml, respectively. Among the larval instars, instar II was the most susceptible. A considerable repellency was noted; a 10 mg/l concentration of S. wightii showed a repellency of 89%. Sea weed extract and B. thuringiensis toxins affected the larval duration and adult emergence. A synergistic factor was also found for the effect of seaweed extract against larvae and pupae of mosquito. The synergistic factor showed at I Instar was 1.74, II Instar was 1.93, III Instar was 1.37, IV Instar was 1.27, and pupa was 1.24, respectively. The result revealed that the seaweed extract of S. wightii in combination with microbial toxins has interfered in the gut system and resulted in mortality as well as growth inhibitory effects on mosquitoes. C1 [Kumar, Kanagarajan Prasanna; Murugan, Kadarkarai; Kovendan, Kalimuthu; Kumar, Arjunan Naresh] Bharathiar Univ, Dept Zool, Sch Life Sci, Coimbatore 641046, Tamil Nadu, India. [Hwang, Jiang-Shiou] Natl Taiwan Ocean Univ, Inst Marine Biol, Keelung 20224, Taiwan. [Barnard, Donald R.] USDA ARS CMAVE, Gainesville, FL 32608 USA. RP Murugan, K (reprint author), Bharathiar Univ, Dept Zool, Sch Life Sci, Coimbatore 641046, Tamil Nadu, India. EM kmvvk@yahoo.com NR 29 TC 10 Z9 10 U1 1 U2 7 PU THAILANDS NATL SCIENCE & TECHNOLOGY DEVELOPMENT AGENCY PI BANGKOK PA PUBLIC INFORMATION DEPT, 73/1 RAMA VI RD, RAJDHEVEE, BANGKOK, 00000, THAILAND SN 1513-1874 J9 SCIENCEASIA JI Scienceasia PD JUN PY 2012 VL 38 IS 2 BP 141 EP 146 DI 10.2306/scienceasia1513-1874.2012.38.141 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 979MH UT WOS:000306823600003 ER PT J AU Brown, DJ Schultz, AD Dixon, JR Dickerson, BE Forstner, MRJ AF Brown, Donald J. Schultz, Amanda D. Dixon, James R. Dickerson, Brian E. Forstner, Michael R. J. TI Decline of Red-Eared Sliders (Trachemys scripta elegans) and Texas Spiny Softshells (Apalone spinifera emoryi) in the Lower Rio Grande Valley of Texas SO CHELONIAN CONSERVATION AND BIOLOGY LA English DT Article ID ECONOMIC-GROWTH; CRYPTOBRANCHUS-ALLEGANIENSIS; TURTLE CONSERVATION; POPULATION DECLINES; URBANIZATION; RIVER; BIODIVERSITY; MANAGEMENT; CALIFORNIA; COMMUNITY AB In 2009, we repeated a freshwater turtle survey first conducted in 1976 in the Lower Rio Grande Valley (LRGV) of Texas to determine whether the abundance of freshwater turtles in the LRGV has changed over the past three decades. We captured significantly fewer red-eared sliders (Trachemys scripta elegans) and Texas spiny softshells (Apalone spinifera emoryi) in 2 recently urbanized counties (Cameron and Hidalgo), and more red-eared sliders and Texas spiny softshells in a nonurbanized county (Willacy). Land-use changes, increased urbanizatiion, and commercial turtle harvest are likely responsible for the decline of freshwater turtles in the LRGV. C1 [Brown, Donald J.; Schultz, Amanda D.; Forstner, Michael R. J.] Texas State Univ, Dept Biol, San Marcos, TX 78666 USA. [Dixon, James R.] Texas A&M Univ, Dept Wildlife & Fisheries Sci, College Stn, TX 77843 USA. [Dickerson, Brian E.] US Forest Serv, USDA, Rocky Mt Res Stn, Rapid City, SD 57702 USA. RP Brown, DJ (reprint author), Texas State Univ, Dept Biol, 601 Univ Dr, San Marcos, TX 78666 USA. EM db1300@txstate.edu; as1745@txstate.edu; jrdixontcwc@gmail.com; bedickerson@fs.fed.us; mf@txstate.edu FU Texas Parks and Wildlife Department FX We thank the public and private agencies and landowners who allowed us to conduct this research on their properties. B. DeVolld, J. Gaertner, R. Holihan, J. Flores, J. Duvall, and J. Duvall-Jisha assisted in trapping and data collection. M. Pons Jr. and the Nature Conservancy of Texas provided housing. Texas Parks and Wildlife Department provided funding for this study and the larger freshwater turtle assessment in Texas. We conducted this research under Texas Parks and Wildlife Department permit SPR-0102-191. Trapping and handling methods were approved by the Texas State University-San Marcos Institutional Animal Care and Use Committee (Protocol 0715-0428-07). Two anonymous reviewers provided comments that improved the manuscript. NR 50 TC 2 Z9 2 U1 3 U2 17 PU CHELONIAN RESEARCH FOUNDATION PI LUNENBURG PA 168 GOODRICH ST., LUNENBURG, MA USA SN 1071-8443 J9 CHELONIAN CONSERV BI JI Chelonian Conserv. Biol. PD JUN PY 2012 VL 11 IS 1 BP 138 EP 143 PG 6 WC Zoology SC Zoology GA 975HF UT WOS:000306500600019 ER PT J AU Hoffmann, WC Walker, TW Fritz, BK Farooq, M Smith, VL Robinson, CA Lan, YB AF Hoffmann, W. Clint Walker, Todd W. Fritz, Bradley K. Farooq, Muhammad Smith, Vincent L. Robinson, Cathy A. Lan, Yubin TI FURTHER EVALUATION OF SPRAY CHARACTERIZATION OF SPRAYERS TYPICALLY USED IN VECTOR CONTROL SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Atomization; droplet size; sprayer; vector control; climate change ID ULTRA-LOW-VOLUME; ADULT MOSQUITOS; DROPLET-SIZE; EQUIPMENT; MALATHION; AEROSOLS AB This work reports droplet-size data measured as part of a collaborative testing program between the US Department of Agriculture, Agricultural Research Service, and the US Navy, Navy Entomological Center for Excellence. This is an ongoing relationship that seeks to test new and revised spray technologies that may potentially be used by deployed personnel. As new equipment comes to market or when existing equipment is modified they are all integrated into this annual testing. During the 2011 equipment evaluations, 24 sprayers were operated across their range of available settings (pressure and flow rate), using both water and oil solutions. Droplet-size data as measured with laser diffraction ranged from 4 to 223 mm (volume median diameter). Generally, as the spray rate increased, droplet size increased, and as the pressure increased at a given same spray rate, droplet size decreased. This information allows users to set up and operate these sprayers in a manner such that a particular droplet size is applied optimizing efficiency and efficacy of applications. C1 [Hoffmann, W. Clint; Fritz, Bradley K.; Lan, Yubin] USDA ARS, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. [Walker, Todd W.; Farooq, Muhammad; Smith, Vincent L.; Robinson, Cathy A.] USN, Entomol Ctr Excellence, Jacksonville, FL 32212 USA. RP Hoffmann, WC (reprint author), USDA ARS, Areawide Pest Management Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. FU Deployed War-Fighter Protection Research Program; US Department of Defense through the Armed Forces Pest Management Board FX This study was supported in part by a grant from the Deployed War-Fighter Protection Research Program, funded by the US Department of Defense through the Armed Forces Pest Management Board. The authors would like to thank Chris Parker for his assistance during the test and Wilbur Ellis for supplying the R-11 for these tests and the equipment manufacturers for their cooperation. NR 18 TC 2 Z9 2 U1 0 U2 6 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD JUN PY 2012 VL 28 IS 2 BP 93 EP 101 DI 10.2987/11-6109.1 PG 9 WC Entomology SC Entomology GA 976BV UT WOS:000306556400004 PM 22894119 ER PT J AU Clark, GG Rubio-Palis, Y AF Clark, Gary G. Rubio-Palis, Yasmin TI MOSQUITO VECTOR BIOLOGY AND CONTROL IN LATIN AMERICA-A 22ND SYMPOSIUM SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Dengue; malaria; surveillance; chemical control; bionomics; insecticide resistance; Aedes; Anopheles; Culex; Leishmania ID ABSTRACTS AB The 22nd Annual Latin American Symposium presented by the American Mosquito Control Association (AMCA) was held as part of the 78th Annual Meeting in Austin, TX, in February 2012. The principal objective, as for the previous 21 symposia, was to promote participation in the AMCA by vector control specialists, public health workers, and academicians from Latin America. This publication includes summaries of 21 presentations that were given orally in Spanish or presented as posters by participants from Mexico, Colombia, Venezuela, and the USA. Topics addressed in the symposium included surveillance, chemical control, insecticide resistance, and genetics associated with Aedes aegypti; food sources and control of Culex; taxonomy, surveillance, and control of Anopheles vectors of malaria; and studies of dengue virus and Leishmania. C1 [Clark, Gary G.] ARS, Mosquito & Fly Res Unit, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Rubio-Palis, Yasmin] Univ Carabobo, Minist Poder Popular Salud & BIOMED, Direcc Salud Ambiental, Maracay, Aragua, Venezuela. RP Clark, GG (reprint author), ARS, Mosquito & Fly Res Unit, Ctr Med Agr & Vet Entomol, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. FU Clarke; Valent Biosciences Corporation; Univar USA FX Financial support for the 22nd Annual Symposium was provided by the following sponsors: Clarke, Valent Biosciences Corporation, and Univar USA. Funds from these sponsors provided support for Annual Meeting registration for some participants, for the Latin American Student Competition, and publication of these summaries. The continuing support by the AMCA for the symposium is also acknowledged. In addition, Manuel Lluberas is acknowledged for his support and dedication to the annual symposium and for utilizing his excellent simultaneous translation skills. Henry Rupp is acknowledged for his excellent editorial assistance with the summaries submitted by symposium participants. Enthusiasm and interest for this symposium among Spanish- and non-Spanish-speaking participants was high and it will continue to be a part of future meetings. NR 19 TC 4 Z9 4 U1 1 U2 8 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X EI 1943-6270 J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD JUN PY 2012 VL 28 IS 2 BP 102 EP 110 DI 10.2987/12-6253.1 PG 9 WC Entomology SC Entomology GA 976BV UT WOS:000306556400005 PM 22894120 ER PT J AU Kline, DL Bernier, UR Hogsette, JA AF Kline, Daniel L. Bernier, Ulrich R. Hogsette, Jerome A. TI EFFICACY OF THREE ATTRACTANT BLENDS TESTED IN COMBINATION WITH CARBON DIOXIDE AGAINST NATURAL POPULATIONS OF MOSQUITOES AND BITING FLIES AT THE LOWER SUWANNEE WILDLIFE REFUGE SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Article DE Aedes taeniorhynchus; Culex nigripalpus; Culicoides; Diachlorus ferrugatus; Lutzomyia shannoni ID L-LACTIC ACID; AEDES-AEGYPTI DIPTERA; HUMAN SKIN EMANATIONS; HOST-SEEKING; 1-OCTEN-3-OL; CULICIDAE; TRAPS; HUMANS; SURVEILLANCE; RESPONSES AB Synthetic blends of chemicals identified previously from human skin emanations were evaluated against mosquito and biting fly populations at the Lower Suwannee Wildlife Refuge near Cedar Key, FL. Mosquito Magnet (TM)-Experimental traps were baited with the Red (400 ml acetone: 10 ml 1-hexen-3-ol: 10 ml 1-octen-3-ol), Blue (400 ml acetone: 1 g/liter lactic acid: 20 ml glycolic acid), or Green blend (400 ml acetone: 1.5 g/liter lactic acid: 20 ml dimethyl disulfide) plus CO2 or with CO2 alone (control). A relative index of efficacy was determined by dividing each mean blend trap catch by the mean control trap catch. Five mosquitoes (Aedes infirmatus, Ae. taeniorhynchus, Ae. triseriatus, Anopheles crucians, and Culex nigripalpus), 2 ceratopogonid (Culicoides floridensis and C. furens), and 1 tabanid (Diachlorus ferrugatus) and phlebotomine (Lutzomyia shannoni) species were trapped. The Red blend + CO2 treatment significantly increased collections of Ae. taeniorhynchus (3.43), An. crucians (2.83), total mosquitoes (2.73), C. furens (17.63), and L. shannoni (10.83) compared with control traps. Traps baited with either the Blue or Green blends generally captured fewer insects than traps baited with the other 2 treatments. However, traps baited with the Green blend caught 73 as many C. furens as the control traps. Responses clearly varied according to species; therefore, "one size does not fit all" when it comes to attractant blends. C1 [Kline, Daniel L.; Bernier, Ulrich R.; Hogsette, Jerome A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Kline, DL (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. NR 28 TC 8 Z9 8 U1 1 U2 26 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD JUN PY 2012 VL 28 IS 2 BP 123 EP 127 DI 10.2987/11-6200R.1 PG 5 WC Entomology SC Entomology GA 976BV UT WOS:000306556400010 PM 22894125 ER PT J AU Welch, JB AF Welch, John B. TI 2012 AMCA MEMORIAL LECTURE HONOREE: DR. JAMES DUNCAN LONG 1925-2009 SO JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION LA English DT Biographical-Item C1 USDA, Anim & Plant Hlth Inspect Serv, Washington, DC 20521 USA. RP Welch, JB (reprint author), USDA, Anim & Plant Hlth Inspect Serv, 9100 Panama City Pl, Washington, DC 20521 USA. NR 1 TC 0 Z9 0 U1 0 U2 1 PU AMER MOSQUITO CONTROL ASSOC PI MOUNT LAUREL PA 15000 COMMERCE PARKWAY, SUITE C, MOUNT LAUREL, NJ 08054 USA SN 8756-971X J9 J AM MOSQUITO CONTR JI J. Am. Mosq. Control Assoc. PD JUN PY 2012 VL 28 IS 2 BP 137 EP 144 DI 10.2987/12-6251.1 PG 8 WC Entomology SC Entomology GA 976BV UT WOS:000306556400014 PM 22894129 ER PT J AU Clark, BR Suzuki, JY Bliss, BJ Borris, RP AF Clark, Benjamin R. Suzuki, Jon Y. Bliss, Barbara J. Borris, Robert P. TI Flavone C-glycosides from Anthurium andraeanum SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Anthurium; Flavone; glycoside; 4 '''-(3,4-Dimethoxycinnamoyl)-embinin; 4 '''-Ferruloyl-embinin ID ANTHOCYANINS AB We describe here the isolation of three flavone 6-C-glycosides from the leaves of Anthurium andraeanum, The two new flavones were identified through detailed spectroscopic analysis as 4'''-(3,4-dimethoxycinnamoyl)-embinin (2) and 4'''-ferruloyl-embinin (3). C1 [Clark, Benjamin R.; Borris, Robert P.] Univ Hawaii, Coll Pharm, Hilo, HI 96720 USA. [Suzuki, Jon Y.; Bliss, Barbara J.] ARS, Trop Plant Genet Resources & Dis Res Unit, USDA, Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Borris, RP (reprint author), Univ Hawaii, Coll Pharm, Hilo, HI 96720 USA. EM borris@hawaii.edu RI Clark, Benjamin/M-5570-2015; Borris, Robert/K-1095-2015 OI Clark, Benjamin/0000-0001-8987-7092; Borris, Robert/0000-0002-5317-7382 FU NSF Hawaii EPSCoR Program under National Science Foundation Award [EPS-0903833]; College of Pharmacy, University of Hawaii at Hilo FX This project is supported in part by the NSF Hawaii EPSCoR Program under National Science Foundation Award EPS-0903833 and by the College of Pharmacy, University of Hawaii at Hilo. NR 6 TC 4 Z9 5 U1 2 U2 4 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD JUN PY 2012 VL 7 IS 6 BP 747 EP 748 PG 2 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA 967PW UT WOS:000305921300016 PM 22816298 ER PT J AU Lin, LZ Harnly, JM AF Lin, Long-Ze Harnly, James M. TI LC-PDA-ESI/MS Identification of the Phenolic Components of Three Compositae Spices: Chamomile, Tarragon, and Mexican Arnica SO NATURAL PRODUCT COMMUNICATIONS LA English DT Article DE Chamomile; Tarragon; Mexican arnica; Flavonoids; Caffeoylquinic acids; LC-PDA-ESI/MS ID HETEROTHECA-INULOIDES; ARTEMISIA-DRACUNCULUS; EXTRACTS; RECUTITA; FLOWERS; TEA; CONSTITUENTS; DERIVATIVES; FLAVONOIDS; GLYCOSIDES AB Chamomile (Matricaria chamomilla L.), tarragon (Artemisia dracunculus L.) and Mexican arnica (Heterotheca inuoides) are common compositae spices and herbs found in the US market. They contain flavonoids and hydroxycinnamates that are potentially beneficial to human health. A standardized LC-PDA-ESI/MS profiling method was used to identify 51 flavonoids and 17 hydroxycinnamates. Many of the identifications were confirmed with authentic standards or through references in the literature or the laboratory's database. More than half of the phenol compounds for each spice had not been previously reported. The phenolic profile can be used for plant authentication and to correlate with biological activities. C1 [Lin, Long-Ze; Harnly, James M.] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Lin, LZ (reprint author), ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, 10300 Baltimore Ave,Bldg 161,BARC E, Beltsville, MD 20705 USA. EM Longze.Lin@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements at the National Institutes of Health FX This research was supported by the Agricultural Research Service of the U.S. Department of Agriculture and the Office of Dietary Supplements at the National Institutes of Health under an Interagency Agreement. NR 27 TC 8 Z9 8 U1 1 U2 12 PU NATURAL PRODUCTS INC PI WESTERVILLE PA 7963 ANDERSON PARK LN, WESTERVILLE, OH 43081 USA SN 1934-578X J9 NAT PROD COMMUN JI Nat. Prod. Commun. PD JUN PY 2012 VL 7 IS 6 BP 749 EP 752 PG 4 WC Chemistry, Medicinal; Food Science & Technology SC Pharmacology & Pharmacy; Food Science & Technology GA 967PW UT WOS:000305921300017 PM 22816299 ER PT J AU Shen, CL Cao, JJ Dagda, RY Chanjaplammootil, S Lu, CW Chyu, MC Gao, WM Wang, JS Yeh, JK AF Shen, Chwan-Li Cao, Jay J. Dagda, Raul Y. Chanjaplammootil, Samuel Lu, Chuanwen Chyu, Ming-Chien Gao, Weimin Wang, Jia-Sheng Yeh, James K. TI Green tea polyphenols benefits body composition and improves bone quality in long-term high-fat diet-induced obese rats SO NUTRITION RESEARCH LA English DT Article DE Tea; Obesity; Body composition; Bone strength; Anti-inflammation; Antioxidant; Rat ID X-RAY ABSORPTIOMETRY; DUCHENNE MUSCULAR-DYSTROPHY; ACTIVATED-RECEPTOR-GAMMA; MESENCHYMAL STEM-CELLS; AGED FEMALE RATS; ADIPOSE-TISSUE; METABOLIC SYNDROME; MINERAL DENSITY; CORTICAL BONE; MECHANICAL-PROPERTIES AB This study investigates the effects of green tea polyphenols (GTPs) on body composition and bone properties along with mechanisms in obese female rats. Thirty-six 3-month-old Sprague Dawley female rats were fed either a low-fat (LF) or a high-fat (HF) diet for 4 months. Animals in the LF diet group continued on an LF diet for additional 4 months, whereas those in the HF diet group were divided into 2 groups: with GTP (0.5%) or without in drinking water, in addition to an HF diet for another 4 months. Body composition, femur bone mass and strength, serum endocrine and proinflammatory cytokines, and liver glutathione peroxidase (GPX) protein expression were determined. We hypothesized that supplementation of GTP in drinking water would benefit body composition, enhance bone quality, and suppress obesity-related endocrines in HF diet-induced obese female rats and that such changes are related to an elevation of antioxidant capacity and a reduction of proinflammatory cytokine production. After 8 months, compared with the LF diet, the HF diet increased percentage of fat mass and serum insulin-like growth factor I and leptin levels; reduced percentage of fat-free mass, bone strength, and GPX protein expression; but had no effect on bone mineral density and serum adiponectin levels in the rats. Green tea polyphenol supplementation increased percentage of fat-free mass, bone mineral density and strength, and GPX protein expression and decreased percentage of fat mass, serum insulin-like growth factor I, leptin, adiponectin, and proinflammatory cytokines in the obese rats. This study shows that GTP supplementation benefited body composition and bone properties in obese rats possibly through enhancing antioxidant capacity and suppressing inflammation. (C) 2012 Elsevier Inc. All rights reserved. C1 [Shen, Chwan-Li; Dagda, Raul Y.] Texas Tech Univ Hlth Sci Ctr, Dept Pathol, Lubbock, TX 79430 USA. [Shen, Chwan-Li] Texas Tech Univ Hlth Sci Ctr, Laura W Bush Inst Womens Hlth, Lubbock, TX 79430 USA. [Cao, Jay J.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58201 USA. [Chanjaplammootil, Samuel; Chyu, Ming-Chien] Texas Tech Univ, Whitacre Coll Engn, Healthcare Engn Grad Program, Lubbock, TX 79409 USA. [Lu, Chuanwen; Gao, Weimin] Texas Tech Univ, Inst Environm & Human Hlth, Lubbock, TX 79409 USA. [Lu, Chuanwen; Gao, Weimin] Texas Tech Univ, Dept Environm Toxicol, Lubbock, TX 79409 USA. [Wang, Jia-Sheng] Univ Georgia, Dept Environm Hlth Sci, Athens, GA 30602 USA. [Yeh, James K.] Winthrop Univ Hosp, Mineola, NY 11501 USA. RP Shen, CL (reprint author), Texas Tech Univ Hlth Sci Ctr, Dept Pathol, 1A089,3601 4th St, Lubbock, TX 79430 USA. EM leslie.shen@ttuhsc.edu FU Laura W. Bush Institute for Women's Health; Winthrop-University Hospital; USDA Agricultural Research Service (ARS) CRIS program [5450-51000-046-00D, 5450-51000-039-00D] FX This study was supported by Laura W. Bush Institute for Women's Health and Winthrop-University Hospital (CLS) and partially supported by the USDA Agricultural Research Service (ARS) CRIS program 5450-51000-046-00D and 5450-51000-039-00D (JJC). NR 101 TC 25 Z9 27 U1 1 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUN PY 2012 VL 32 IS 6 BP 448 EP 457 DI 10.1016/j.nutres.2012.05.001 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 973XE UT WOS:000306392800008 PM 22749181 ER PT J AU Yan, L DeMars, LC Johnson, LK AF Yan, Lin DeMars, Lana C. Johnson, LuAnn K. TI Long-term voluntary running improves diet-induced adiposity in young adult mice SO NUTRITION RESEARCH LA English DT Article DE Running; Adiposity; Inflammation; Angiogenesis; Mice ID PLASMINOGEN-ACTIVATOR INHIBITOR-1; MONOCYTE CHEMOATTRACTANT PROTEIN-1; INSULIN-RESISTANCE; ENDOTHELIAL-CELLS; PROMOTES ANGIOGENESIS; TISSUE INFLAMMATION; PHYSICAL-ACTIVITY; OBESE MICE; FAT DIET; EXERCISE AB The hypothesis of the present study was that physical activity improves diet-induced obesity in young adult mice. Four-week-old male C57BL/6 mice. (n = 15/group) were fed the AIN93G diet or a 45% high-fat diet (% kJ) with or without access to in-cage activity wheels for 14 weeks. The high-fat diet increased percentage fat body mass compared to the AIN93G diet (P=.042); running reduced percentage fat body mass (P<.0001) and increased percentage lean body mass (P<.0001) in mice fed either diet. Compared with the AIN93G diet, the high-fat diet increased plasma concentrations of insulin (P<.05) and leptin (P<.05) in sedentary mice and inflammatory cytokines monocyte chemotactic protein-1 (MCP-1) (P<.05) and plasminogen activator inhibitor-1 (P<.05) in both sedentary and running mice. The high-fat diet did not affect angiogenic factors vascular endothelial growth factor and platelet-derived growth factor-BB. Running reduced plasma insulin (P<.05) and MCP-1 (P<.05) and increased platelet-derived growth factor-BB (P<.05) in mice fed the high-fat diet. Running reduced leptin (P<.05) and increased plasma vascular endothelial growth factor (P<.0001) regardless of diet fed. In summary, consumption of the high-fat diet increased adiposity in young adult mice; running reduced adiposity, normalized plasma insulin and leptin, and reduced MCP-1 despite continued consumption of the high-fat diet. These results suggest that voluntary running may reduce diet-induced obesity and proinflammation and that young mice may be a useful model of their human age equivalents in studying moderate physical exercise and obesity and obesity-related diseases. Published by Elsevier Inc. C1 [Yan, Lin; DeMars, Lana C.; Johnson, LuAnn K.] USDA, Agr Res Serv, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. RP Yan, L (reprint author), USDA, Agr Res Serv, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. EM lin.yan@ars.usda.gov OI yan, lin/0000-0003-3646-6374 FU USDA, ARS [5450-51000-036-00D] FX The authors greatly acknowledge the assistance of the following staff of Grand Forks Human Nutrition Research Center: James Linlauf for preparing experimental diets, Kay Keehr for technical support, and the vivarium staff for providing high-quality animal care. Funding for this work was provided by the USDA, ARS, Research project 5450-51000-036-00D. The USDA, ARS, Northern Plains Area is an equal opportunity/affirmative action employer; and all agency services are available without discrimination. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the USDA. NR 49 TC 9 Z9 11 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUN PY 2012 VL 32 IS 6 BP 458 EP 465 DI 10.1016/j.nutres.2012.05.006 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 973XE UT WOS:000306392800009 PM 22749182 ER PT J AU King, DI Chandler, CC Rappole, JH Chandler, RB Mehlman, DW AF King, David I. Chandler, Carlin C. Rappole, John H. Chandler, Richard B. Mehlman, David W. TI Establishing quantitative habitat targets for a 'Critically Endangered' Neotropical migrant (Golden-cheeked Warbler Dendroica chrysoparia) during the non-breeding season SO BIRD CONSERVATION INTERNATIONAL LA English DT Article ID HIERARCHICAL-MODELS; CONSERVATION; ABUNDANCE; ECOLOGY AB The Golden-checked Warbler Dendroica chrysoparia is a federally endangered Neotropical migrant that inhabits montane pine-oak forests in Mexico and northern Central America during the non-breeding season. Although it is known that Golden-checked Warblers are closely associated with 'encino' oaks (evergreen or holm oak) such as Quercus sapotifolia, Q. eliptica and Q. elongata, which have shiny, narrow, elliptical, or oblong leaves, quantitative habitat targets are useful for effectively incorporating this information into conservation planning and forest management practices. We analysed data on wintering Golden-checked Warblers collected during the non-breeding season in Honduras from 1996 to 1998 to identify quantitative targets for habitat conditions for this species. Data on warbler abundance were collected using line transect surveys located in montane pine-oak forests in a stratified-random fashion. Habitat data were collected at five 0.04 ha plots on these same transects and the averaged values used as predictors of Golden-checked Warbler abundance. We found that Golden-checked Warblers were strongly associated with the basal area of encino oaks and density of 'roble' oaks, such as Q. segoviensis, Q. purulhana and Q. rugosa, which have large, lobed leaves. Density of Golden-checked Warblers peaked at approximate to 5.6 m(-2) ha(-1) basal area of encino and approximate to 7 roble oaks ha(-1). These values can be used to identify quantitative habitat targets that can be directly incorporated into forest management practices to ensure that these activities maintain habitat conditions necessary for their use by Golden-checked Warblers. C1 [King, David I.] Univ Massachusetts, No Res Stn, USDA, US Forest Serv, Amherst, MA 01003 USA. [Chandler, Carlin C.; Chandler, Richard B.] Univ Massachusetts, Dept Nat Resources Conservat, Amherst, MA 01003 USA. [Rappole, John H.] Conservat Res Ctr, Smithsonian Inst, Front Royal, VA 22630 USA. [Mehlman, David W.] Nature Conservancy, Migratory Bird Program, Albuquerque, NM 87104 USA. RP King, DI (reprint author), Univ Massachusetts, No Res Stn, USDA, US Forest Serv, 160 Holdsworth Way,202 Holdsworth Hall, Amherst, MA 01003 USA. EM dking@fs.fed.us RI Chandler, Richard/D-8831-2014; Chandler, Richard/F-9702-2016 FU USDA Forest Service International Program; The Nature Conservancy Migratory Bird Program FX We thank the USDA Forest Service International Program who funded this work through a cooperative agreement with The Nature Conservancy Migratory Bird Program. We also thank Edgard Herrera for his valuable advice and support, Martha Moreno, Marvin Martinez, and Julio Merida for their help with fieldwork 2006-2008, and Scott Schlossberg and Mitch Hartley for his comments on an earlier draft of this manuscript. NR 20 TC 0 Z9 0 U1 1 U2 29 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0959-2709 J9 BIRD CONSERV INT JI Bird Conserv. Int. PD JUN PY 2012 VL 22 IS 2 BP 213 EP 221 DI 10.1017/S095927091100027X PG 9 WC Ornithology SC Zoology GA 971MP UT WOS:000306208300010 ER PT J AU Zhan, GM Chen, XM Kang, ZS Huang, LL Wang, MN Wan, AM Cheng, P Cao, SQ Jin, SL AF Zhan, Gangming Chen, Xianming Kang, Zhensheng Huang, Lili Wang, Meinan Wan, Anmin Cheng, Peng Cao, Shiqin Jin, Shelin TI Comparative virulence phenotypes and molecular genotypes of Puccinia striiformis f. sp tritici, the wheat stripe rust pathogen in China and the United States SO FUNGAL BIOLOGY LA English DT Article DE Phylogenetic relationship; Puccinia striiformis f. sp tritici; Stripe rust; Yellow rust ID 12 MICROSATELLITE LOCI; F.SP TRITICI; ENRICHMENT PROTOCOL; NORTH-AMERICA; DIVERSITY; RACES; RESISTANCE; STRAINS; FUNGUS; DNA AB Stripe rust (yellow rust) of wheat, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases in both China and the United States. The Chinese and US populations of the stripe rust fungus were compared for their virulence phenotypes on wheat cultivars used to differentiate races of the pathogen in China and the US and molecular genotypes using simple sequence repeat (SSR) markers. From 86 Chinese isolates, 54 races were identified based on reactions on the 17 Chinese differentials and 52 races were identified based on the 20 US differentials. The selected 51 US isolates, representing 50 races based on the US differentials, were identified as 41 races using the Chinese differentials. A total of 132 virulence phenotypes were identified from the 137 isolates based on reactions on both Chinese and US differentials. None of the isolates from the two countries had identical virulence phenotypes on both sets of differentials. From the 137 isolates, SSR markers identified 102 genotypes, of which 71 from China and 31 from the US. The virulence data clustered the 137 isolates into 20 virulence groups (VGs) and the marker data clustered the isolates into seven molecular groups (MGs). Virulence and SSR data had a low (r = 0.34), but significant (P = 0.01) correlation. Principal component analyses using either the virulence data or the SSR data separated the isolates into three groups: group a consisting of only Chinese isolates, group b consisting of both Chinese and US isolates and group c consisting of mostly US isolates. A neighbour-joining tree generated using the molecular data suggested that the P. striiformis f. sp. tritici populations of China and the US in general evolved independently. Published by Elsevier Ltd on behalf of The British Mycological Society. C1 [Zhan, Gangming; Chen, Xianming; Wang, Meinan; Wan, Anmin; Cheng, Peng] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Zhan, Gangming; Kang, Zhensheng; Huang, Lili; Wang, Meinan] NW A&F Univ, State Key Lab Crop Stress Biol Arid Areas & Coll, Yangling, Shaanxi, Peoples R China. [Chen, Xianming] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. [Chen, Xianming] Washington State Univ, Dis Res Unit, Pullman, WA 99164 USA. [Cao, Shiqin; Jin, Shelin] Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou, Gansu, Peoples R China. RP Chen, XM (reprint author), Washington State Univ, Dept Plant Pathol, 361 Johnson Hall, Pullman, WA 99164 USA. EM xianming@wsu.edu; kangzs@nwsuaf.edu.cn RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 FU US Department of Agriculture, Agricultural Research Service [5348-22000-014-00D]; Washington Wheat Commission [13C-3061-3923]; PPNS [0564]; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center [WNP00823]; Washington State University, Pullman [WA 99164-6430]; USA; Agro-scientific Research in the Public Interest of China [200903035-02]; Ministry of Education of China [B07049] FX This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-014-00D) and Washington Wheat Commission (Project No. 13C-3061-3923). PPNS No. 0564, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Project Number WNP00823, Washington State University, Pullman, WA 99164-6430, USA and special fund for Agro-scientific Research in the Public Interest of China (200903035-02) and the 111 Project from the Ministry of Education of China (B07049). We thank Dr Weidong Chen and Dr Jayaveeramuthu Nirmala for critical review of the manuscript. NR 42 TC 11 Z9 11 U1 1 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 J9 FUNGAL BIOL-UK JI Fungal Biol. PD JUN PY 2012 VL 116 IS 6 BP 643 EP 653 DI 10.1016/j.funbio.2012.03.004 PG 11 WC Mycology SC Mycology GA 969YT UT WOS:000306096000002 PM 22658310 ER PT J AU Baumgartner, K Baker, BR Korhonen, K Zhao, J Hughes, KW Bruhn, J Bowman, TS Bergemann, SE AF Baumgartner, Kendra Baker, Bethany R. Korhonen, Kari Zhao, Jun Hughes, Karen W. Bruhn, Johann Bowman, Tiffany S. Bergemann, Sarah E. TI Evidence of natural hybridization among homothallic members of the basidiomycete Armillaria mellea sensu stricto SO FUNGAL BIOLOGY LA English DT Article DE Basidiomycete; Introgressive hybridization; Mating system; Mito-nuclear discordance ID AGARICUS-BISPORUS; HAPLOTYPE RECONSTRUCTION; CRINIPELLIS-PERNICIOSA; SOUTH-AFRICA; LIFE-CYCLE; DNA; POPULATION; FUNGI; IDENTIFICATION; DIVERSITY AB Populations of Armillaria mellea (Basidiomycota, Agaricales) across much of its range are heterothallic; homothallic populations occur only in Africa (A. mellea ssp. africana), China (China Biological Species CBS G), and Japan (A. mellea ssp. nipponica). Monosporous isolates of heterothallic A. mellea are haploid and their mating behaviour is consistent with the requirement of two different alleles at two mating-type loci (tetrapolar mating system) to create a diploid individual. In contrast, monosporous isolates of homothallic A. mellea are putatively diploid; they bypass the haploid phase by undergoing karyogamy in the basidium (a unique type of secondary homothallism/pseudohomothallism). In order to determine the genetic origin of this homothallism, we analyzed genetic variation of 47 heterothallic isolates from China, Europe, and North America, and 14 homothallic isolates from Africa, China, and Japan. Gene trees and mutational networks were constructed for partial mitochondrial gene ATP synthase subunit 6 (ATP6) and for the following nuclear genes: actin (ACTIN), elongation factor subunit 1-alpha (EFA), glyceraldehyde 3-phosphate dehydrogenase (GPD), and the RNA polymerase subunit II (RPB2). Homothallic isolates from Africa and Japan shared a common mitochondrial ATP6 haplotype with homothallic isolates from China, and are likely introductions. Homothallic isolates from China that shared a common mitochondrial haplotype with all European isolates did not share European nuclear haplotypes, as revealed by median-joining networks, but instead clustered with haplotypes from China or were intermediate between those of China and Europe. Such mitochondrial-nuclear discordance in homothallic isolates from China is indicative of hybridization between lineages originating from China and Europe. (C) 2012 British Mycological Society. Published by Elsevier Ltd. All rights reserved. C1 [Baker, Bethany R.; Bowman, Tiffany S.; Bergemann, Sarah E.] Middle Tennessee State Univ, Dept Biol, Murfreesboro, TN 37132 USA. [Baumgartner, Kendra] Univ Calif Davis, ARS, USDA, Davis, CA 95616 USA. [Korhonen, Kari] Finnish Forest Res Inst, FI-01301 Vantaa, Finland. [Zhao, Jun] State Forestry Adm, Gen Stn Forest Pest Control, Shenyang 110034, Peoples R China. [Hughes, Karen W.] Univ Tennessee, Knoxville, TN 37996 USA. [Bruhn, Johann] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. RP Bergemann, SE (reprint author), Middle Tennessee State Univ, Dept Biol, POB 60, Murfreesboro, TN 37132 USA. EM sbergema@mtsu.edu FU STEPMT; Faculty Research and Creative Activity; National Science Foundation Major Research Instrumentation [DBI-0922922]; USDA-NIFA FX We thank R. Peterson (University of Tennessee, Knoxville), J. Micales (USDA-FS Forest Products Lab, Madison, WI), and M. Binder (Clark University, Worcester, MA) for collections; and T. McClean (Nucleic Acid Exploration Facility, University of Wyoming, Laramie) for sequencing. Funding was provided by STEPMT (B.R. Baker), Faculty Research and Creative Activity (S.E. Bergemann), National Science Foundation Major Research Instrumentation DBI-0922922 (S.E. Bergemann), and USDA-NIFA (K. Baumgartner). NR 60 TC 5 Z9 5 U1 4 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 J9 FUNGAL BIOL-UK JI Fungal Biol. PD JUN PY 2012 VL 116 IS 6 BP 677 EP 691 DI 10.1016/j.funbio.2012.03.006 PG 15 WC Mycology SC Mycology GA 969YT UT WOS:000306096000005 PM 22658313 ER PT J AU Skinner, DZ AF Skinner, Daniel Z. TI Genetics of winter wheat response to two freezing treatments SO PLANT BREEDING LA English DT Article DE cold; freeze-thaw; freezing tolerance; wheat ID COLD-ACCLIMATION; SATURATED SOIL; TOLERANCE; TEMPERATURE; HARDINESS; SURVIVAL; EXPOSURE; FROST AB With 1 figure and 3 tables Abstract The inheritance of the ability of winter wheat (Triticum aestivum) plants to survive two kinds of freezing stress was investigated in a five-parent diallel cross. Cold-acclimated plants were frozen to damaging temperatures with or without a -3 degrees C, 16-h prefreezing (PF) period. Test temperatures were adjusted such that the overall mean survivals were the same under the two PF treatment conditions (-10 to -12 degrees C without PF; -14.5 to -16.5 degrees C with PF). Lines that survived freezing tests with PF as well or better than without PF developed significantly more freezing tolerance during the PF treatment and tolerated the lower test temperatures of the PF tests. Diallel analysis indicated most variation under either kind of freezing stress was because of additive genetic effects, but non-additive effects were significant in two of the 20 crosses. It may be possible to combine additive and non-additive effects to develop cultivars that are exceptionally tolerant of freezing episodes with or without an extended PF period. C1 [Skinner, Daniel Z.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Skinner, Daniel Z.] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. RP Skinner, DZ (reprint author), Washington State Univ, USDA ARS, Pullman, WA 99164 USA. EM dan.skinner@ars.usda.gov FU USDA-ARS [5348-21430-003-00D] FX The author thanks Brian Bellinger for excellent technical assistance. This project was supported by USDA-ARS project 5348-21430-003-00D. Mention of product names does not represent an endorsement of any product or company but is given only to clarify the methodology; other products may be equally effective. NR 23 TC 0 Z9 0 U1 1 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2012 VL 131 IS 3 BP 380 EP 384 DI 10.1111/j.1439-0523.2012.01954.x PG 5 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 953WP UT WOS:000304904600004 ER PT J AU Chambers, A Whitaker, VM Gibbs, B Plotto, A Folta, KM AF Chambers, Alan Whitaker, Vance M. Gibbs, Brian Plotto, Anne Folta, Kevin M. TI Detection of the linalool-producing NES1 variant across diverse strawberry (Fragaria spp.) accessions SO PLANT BREEDING LA English DT Article DE Fragaria; strawberry; flavour; linalool; nerolidol synthase; molecular marker ID VOLATILE COMPOUNDS; WILD STRAWBERRIES; AROMA COMPOUNDS; QUALITY AB With 4 figures and 3 tables Abstract Many volatile compounds have been shown to influence the flavour of strawberry (Fragaria spp.) fruit. A published study demonstrated that linalool, a critical flavour compound, is produced in cultivated F. X ananassa varieties owing to a truncated form of the NEROLIDOL SYNTHASE (NES) enzyme. The corresponding allele (FaNES1) features a 5'-deletion that is detectable by PCR. Herein, we tested a broad set of strawberry genotypes to understand the origin of the allele and to identify materials that may be preferred candidates for molecular breeding. The results indicate that the FaNES1 allele (linalool+) is not present in any diploid, tetraploid or hexaploid accession tested. It is present in 112 F. X ananassa cultivars examined. The FaNES1 allele is present in all but three of 46 wild F.similar to virginiana and F.similar to chiloensis genotypes. Two of these three lines continue to produce linalool despite the absence of the allele. This survey suggests that the FaNES1 allele possibly arose following octoploidization and that linalool possibly is an important factor in strawberry biology, aside from its role in anthrocentric fruit quality attributes. C1 [Chambers, Alan; Whitaker, Vance M.; Gibbs, Brian; Folta, Kevin M.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Whitaker, Vance M.] Gulf Coast Res & Educ Ctr, Wimauma, FL 33598 USA. [Plotto, Anne] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Folta, Kevin M.] Univ Florida, Grad Program Plant Mol & Cellular Biol, Gainesville, FL 32611 USA. RP Folta, KM (reprint author), Univ Florida, Dept Hort Sci, 1301 Fifield Hall, Gainesville, FL 32611 USA. EM kfolta@ifas.ufl.edu FU USDA; University of Florida Plant Molecular Breeding Initiative; Florida Strawberry Research and Education Foundation FX The authors thank Dr. Nahla Bassil for supplying the majority of DNA and plant materials that were used in this work, as well as Dr. Thomas M. Davis for his gift of F. gracilis plants. This project was funded by the USDA Florida Specialty Crops Block Grant (KMF and VMW), the University of Florida Plant Molecular Breeding Initiative, and the Florida Strawberry Research and Education Foundation. NR 21 TC 6 Z9 6 U1 0 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 J9 PLANT BREEDING JI Plant Breed. PD JUN PY 2012 VL 131 IS 3 BP 437 EP 443 DI 10.1111/j.1439-0523.2012.01959.x PG 7 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA 953WP UT WOS:000304904600014 ER PT J AU Ramirez, ER Clayton, MC Lawson, CW Burns, SM Guarneros-Altimirano, R DeMaso, SJ Kuvlesky, WP Hewitt, DG Ortega-Santos, JA Campbell, TA AF Ramirez, Eric Reyes Clayton, Megan C. Lawson, Cody W. Burns, Stephen M. Guarneros-Altimirano, Rafael DeMaso, Stephen J. Kuvlesky, William P., Jr. Hewitt, David G. Ortega-Santos, J. Alfonso Campbell, Tyler A. TI HOME RANGES OF FEMALE RIO GRANDE TURKEYS (MELEAGRIS GALLOPAVO INTERMEDIA) IN SOUTHERN TEXAS SO SOUTHWESTERN NATURALIST LA English DT Article ID WILD TURKEYS AB We captured and placed radiotransmitters on 27, 37, and 51 female Rio Grande turkeys (Meleagris gallopavo intermedia) on the Encino, Norias, and Laureles divisions of King Ranch, respectively, in Brooks, Kenedy, and Kleberg counties, Texas. Mean annual sizes of home ranges were 838-5,867 ha, which were larger than most individual holdings of private property in Texas. Conservation plans should take this into consideration. C1 [Campbell, Tyler A.] Texas A&M Univ Kingsville, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv,Natl Wildlife Res Ctr, Kingsville, TX 78363 USA. [DeMaso, Stephen J.] Texas Parks & Wildlife Dept, Austin, TX 78744 USA. [Ramirez, Eric Reyes; Clayton, Megan C.; Lawson, Cody W.; Burns, Stephen M.; Guarneros-Altimirano, Rafael; Kuvlesky, William P., Jr.; Hewitt, David G.; Ortega-Santos, J. Alfonso] Texas A&M Univ Kingsville, Caesar Kleberg Wildlife Res Inst, Kingsville, TX 78363 USA. RP Campbell, TA (reprint author), Texas A&M Univ Kingsville, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv,Natl Wildlife Res Ctr, 700 Univ Blvd,MSC 218, Kingsville, TX 78363 USA. EM tyler.a.campbell@aphis.usda.gov FU Texas Parks and Wildlife Department; National Wild Turkey Federation; Texas Chapter of the National Wild Turkey Federation FX This project was funded by Texas Parks and Wildlife Department, National Wild Turkey Federation, and Texas Chapter of the National Wild Turkey Federation. We appreciate logistical support provided by personnel of King Ranch Incorporated and Caesar Kleberg Wildlife Research Institute at Texas A&M University-Kingsville. We thank J. Delgado-Acevedo for translating the abstract to Spanish. This is manuscript 10-131 of the Caesar Kleberg Wildlife Research Institute. NR 18 TC 0 Z9 0 U1 2 U2 13 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 J9 SOUTHWEST NAT JI Southw. Natural. PD JUN PY 2012 VL 57 IS 2 BP 198 EP 201 PG 4 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 970TY UT WOS:000306156400014 ER PT J AU Squires, JR Olson, LE Turner, DL DeCesare, NJ Kolbe, JA AF Squires, John R. Olson, Lucretia E. Turner, David L. DeCesare, Nicholas J. Kolbe, Jay A. TI Estimating detection probability for Canada lynx Lynx canadensis using snow-track surveys in the northern Rocky Mountains, Montana, USA SO WILDLIFE BIOLOGY LA English DT Article DE Canada lynx; detection probability; forest carnivores; Lynx canadensis; monitoring; snow-track surveys ID ESTIMATING SITE OCCUPANCY; IMPERFECT DETECTION; WESTERN MONTANA; POPULATION; ABUNDANCE; SELECTION; ABSENCE; DISTRIBUTIONS; EXTINCTION; CARNIVORES AB We used snow-tracking surveys to determine the probability of detecting Canada lynx Lynx canadensis in known areas of lynx presence in the northern Rocky Mountains, Montana, USA during the winters of 2006 and 2007. We used this information to determine the minimum number of survey replicates necessary to infer the presence and absence of lynx in areas of similar lynx density (approximately 2.8 lynx/100 km(2)) with confidence. The probability of detecting lynx in mountainous habitats that support resident populations was 0.80-0.99 when surveys were conducted on an 8 x 8 km(2) grid with 10 km of search effort per cell. Snow-track surveys were highly successful at detecting the presence of Canada lynx over large landscapes. Two survey replicates established absence of Canada lynx with 95% certainty. The high probability of detection associated with snow-track surveys makes this method useful for documenting populations of Canada lynx in areas where their status is uncertain. C1 [Squires, John R.; Olson, Lucretia E.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Turner, David L.] US Forest Serv, Rocky Mt Res Stn, Logan, UT 84321 USA. [DeCesare, Nicholas J.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Kolbe, Jay A.] Montana Fish Wildlife & Pk, Seeley Lake, MT 59868 USA. RP Squires, JR (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 E Beckwith, Missoula, MT 59801 USA. EM jsquires@fs.fed.us; lucretiaolson@fs.fed.us; dlturner@fs.fed.us; nick1.decesare@umontana.edu; jkolbe@mt.gov FU Rocky Mountain Research Station, Forestry Sciences Laboratory, Missoula, Montana, USA FX our research was funded by the Rocky Mountain Research Station, Forestry Sciences Laboratory, Missoula, Montana, USA. Our research was conducted in cooperation with Region 1, United States Forest Service and the Bureau of Land Management; logistical support was provided by the Seeley Lake District, Lolo National Forest and the Rexford District, Kootenai National Forest. We thank Jeff Copeland, Kevin McKelvey, Aurelien Besnard, Nicolas Morellet and Mathieu Garel for helpful reviews and suggestions concerning earlier versions of the manuscript. NR 44 TC 1 Z9 1 U1 2 U2 56 PU WILDLIFE BIOLOGY PI RONDE PA C/O JAN BERTELSEN, GRENAAVEJ 14, KALO, DK-8410 RONDE, DENMARK SN 0909-6396 J9 WILDLIFE BIOL JI Wildlife Biol. PD JUN PY 2012 VL 18 IS 2 BP 215 EP 224 DI 10.2981/10-105 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 972BP UT WOS:000306251800010 ER PT J AU Smith, EI Reif, JS Hill, AE Slota, KE Miller, RS Bjork, KE Pabilonia, KL AF Smith, Emily I. Reif, John S. Hill, Ashley E. Slota, Katharine E. Miller, Ryan S. Bjork, Kathe E. Pabilonia, Kristy L. TI Epidemiologic Characterization of Colorado Backyard Bird Flocks SO AVIAN DISEASES LA English DT Article DE avian influenza; backyard flock; epidemiology; zoonotic disease ID INFLUENZA-A H5N1; AVIAN INFLUENZA; NEWCASTLE-DISEASE; POULTRY FARMS; HONG-KONG; OUTBREAK; VIRUS; RISK; INFECTION; SALMONELLOSIS AB Backyard gallinaceous bird flocks may play an important role in the spread of infectious diseases within poultry populations as well as the transmission of zoonotic diseases to humans. An epidemiologic characterization was conducted of Colorado backyard flocks to gather information on general flock characteristics, human movement of birds, human-bird interaction, biosecurity practices, and flock health. Our results suggest that backyard poultry flocks in Colorado are small-sized flocks (68.6% of flocks had <50 birds); consist primarily of layer chickens (85.49% of flocks), show chickens (32.18% of flocks), and waterfowl (34.07% of flocks); and are primarily owned for food (meat or egg) production for the family (86.44%) or as pet or hobby birds (42.27%). The backyard flock environment may promote bird-to-bird transmission as well as bird-to-human transmission of infectious disease. Birds are primarily housed with free access to the outside (96.85%), and many are moved from the home premises (46.06% within 1 yr). Human contact with backyard flocks is high, biosecurity practices are minimal, and bird health is negatively impacted by increased movement events. Increased knowledge of backyard bird characteristics and associated management practices can provide guidelines for the development of measures to decrease disease transmission between bird populations, decrease disease transmission from birds to humans, and increase the overall health of backyard birds. C1 [Smith, Emily I.; Reif, John S.; Slota, Katharine E.; Pabilonia, Kristy L.] Colorado State Univ, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. [Hill, Ashley E.] Univ Calif Davis, Calif Anim Hlth & Food Safety Lab Syst, Davis, CA 95616 USA. [Miller, Ryan S.; Bjork, Kathe E.] Anim & Plant Hlth Inspect Serv, Natl Ctr Risk Anal, Ctr Epidemiol & Anim Hlth, Vet Serv,USDA, Ft Collins, CO 80526 USA. RP Pabilonia, KL (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. EM kristy.pabilonia@colostate.edu OI Miller, Ryan/0000-0003-3892-0251 FU Centers for Disease Control and Prevention [5 U19 CI000419-02] FX We thank the staff of the Colorado Avian Disease Surveillance Program for help with this project. We also thank all of the backyard flock owners that participated in this project. This work was supported by cooperative agreement 5 U19 CI000419-02 from the Centers for Disease Control and Prevention. NR 32 TC 7 Z9 7 U1 3 U2 17 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 263 EP 271 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700001 PM 22856180 ER PT J AU Pantin-Jackwood, MJ Smith, DM Wasilenko, JL Spackman, E AF Pantin-Jackwood, Mary J. Smith, Diane M. Wasilenko, Jamie L. Spackman, Erica TI Low Pathogenicity Avian Influenza Viruses Infect Chicken Layers by Different Routes of Inoculation SO AVIAN DISEASES LA English DT Article DE low pathogenic avian influenza; H6N2 type; H9N2 type; routes of inoculation; chickens ID LAYING HENS; A VIRUSES; TURKEYS; POULTRY; DUCKS; PATHOBIOLOGY; H6N2; SUSCEPTIBILITY; PENNSYLVANIA; PATHOGENESIS AB In order to develop better control measures against avian influenza, it is necessary to understand how the virus transmits in poultry. In a previous study in which the infectivity and transmissibility of the pandemic H1N1 influenza virus was examined in different poultry species, we found that no or minimal infection occurred in chicken and turkeys intranasally (IN) inoculated with the virus. However, we demonstrated that the virus can infect laying turkey hens by the intracloacal (IC) and intraoviduct (IO) routes, possibly explaining the drops in egg production observed in turkey breeder farms affected by the virus. Such novel routes of exposure have not been previously examined in chickens and could also explain outbreaks of low pathogenicity avian influenza (LPAI) that cause a decrease in egg production in chicken layers and breeders. In the present study, 46-wk-old specific-pathogen-free chicken layers were infected by the IN, IC, or 10 routes with one of two LPAI viruses: a poultry origin virus, A/chicken/CA/1255/02 (H6N2), and a live bird market isolate, A/chicken/NJ/12220/97 (H9N2). Only hens IN inoculated with the H6N2 virus presented mild clinical signs consisting of depression and anorexia. However, a decrease in number of eggs laid was observed in all virus-inoculated groups when compared to control hens. Evidence of infection was found in all chickens inoculated with the H6N2 virus by any of the three routes and the virus transmitted to contact hens. On the other hand, only one or two hens from each of the groups inoculated with the H9N2 virus shed detectable levels of virus, or seroconverted and did not transmit the virus to contacts, regardless of the route of inoculation. In conclusion, LPAI viruses can also infect chickens through other routes besides the IN route, which is considered the natural route of exposure. However, as seen with the H9N2 virus, the infectivity of the virus did not increase when given by these alternate routes. C1 [Pantin-Jackwood, Mary J.; Smith, Diane M.; Wasilenko, Jamie L.; Spackman, Erica] ARS, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Pantin-Jackwood, MJ (reprint author), ARS, Exot & Emerging Avian Viral Dis Res Unit, SE Poultry Res Lab, USDA, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Mary.Pantin-Jackwood@ARS.USDA.gov NR 40 TC 8 Z9 8 U1 2 U2 11 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 276 EP 281 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700003 PM 22856182 ER PT J AU Gimeno, IM Cortes, AL Witter, RL Pandiri, AR AF Gimeno, Isabel M. Cortes, Aneg L. Witter, Richard L. Pandiri, Arun R. TI Optimization of the Protocols for Double Vaccination Against Marek's Disease by Using Commercially Available Vaccines: Evaluation of Protection, Vaccine Replication, and Activation of T Cells SO AVIAN DISEASES LA English DT Article DE revaccination; Marek's disease; control; vaccine ID INSERTIONAL MUTAGENESIS; RECOMBINANT HERPESVIRUS; VIRUS FUSION; CHICKENS; CHALLENGE; VIRULENT; DIAGNOSIS; TURKEYS; STRAIN; SUSCEPTIBILITY AB Revaccination against Marek's disease is a widespread practice in some countries. The rationale of this practice is unknown, and there is no consensus in the protocols. Recently, we have demonstrated that administration of the first vaccine at 18 days of embryonation followed by a more protective second vaccine at hatch (18ED/1d) reproduced systematically the benefits of revaccination under laboratory conditions. Here, we have used the same model to optimize the revaccination protocols by using currently available vaccines and to determine whether two features associated with Marek's disease vaccine-induced protection (activation of T cells and replication of vaccine virus) are involved in the revaccination protocols. Protection conferred by three revaccination protocols (turkey herpesvirus [HVT] 18ED/HVT+SB-1 1d, HVT 18ED/CVI988 1d, and HVT+SB-1 I8ED/CVI988 1d) was evaluated. Revaccination protocols also were compared with single vaccination protocols (HVT 18ED, HVT+SB-1 18ED, HVT+SB-1 1d, CVI988 18ED, and CVI988 1d). Our results demonstrated that it is possible to improve efficacy of the currently available vaccines by using them in revaccination programs. Administration of HVT 18ED/CVI988 Id and HVT+SB-1 18ED/CVI988 1d were the two protocols that conferred the highest protection against a very early challenge (2 days of age) with very virulent plus Marek's disease virus strain 648A. In a separate experiment, we evaluated vaccine replication and activation of T cells in single and revaccination protocols. Our results demonstrated that replication of the second vaccine, although decreased compared with single vaccination, could be detected at 3 days (HVT, CVI988) or at 6 days (SB-1). Administration of the first vaccine (HVT) at 18ED resulted in a high percentage of activated T cells. Administration of a second vaccine (either HVI-SB-1 or CVI988) at 1d resulted in increased intensity of MHC-II stain in activated T cells. C1 [Gimeno, Isabel M.; Cortes, Aneg L.; Pandiri, Arun R.] N Carolina State Univ, Dept Populat Hlth & Pathobiol, Coll Vet Med, Raleigh, NC 27607 USA. [Witter, Richard L.] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. RP Gimeno, IM (reprint author), N Carolina State Univ, Dept Populat Hlth & Pathobiol, Coll Vet Med, Raleigh, NC 27607 USA. EM isabel_gimeno@ncsu.edu FU U.S. Poultry and Egg Association [657] FX We thank the U.S. Poultry and Egg Association for funding this study (grant 657). NR 54 TC 5 Z9 5 U1 0 U2 6 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 295 EP 305 PG 11 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700006 PM 22856185 ER PT J AU Jenkins, MC Parker, C Klopp, S O'Brien, C Miska, K Fetterer, R AF Jenkins, Mark C. Parker, Carolyn Klopp, Spangler O'Brien, Celia Miska, Katarzyna Fetterer, Raymond TI Gel-Bead Delivery of Eimeria Oocysts Protects Chickens Against Coccidiosis SO AVIAN DISEASES LA English DT Article DE Eimeria; vaccine; oocysts; gel-bead; spray vaccination ID BROILER-CHICKENS; IMMUNITY; VACCINE; IMMUNIZATION; TENELLA; POULTRY; LEVEL; SENSITIVITY; ACERVULINA; DICLAZURIL AB Vaccines composed of either virulent or attenuated Eimeria spp. oocysts have been developed as an alternative to medication of feed with ionophore drugs or synthetic chemicals. The purpose of this study was to evaluate the use of gel-beads containing a mixture of Eimeria acervulina, Eimeria maxima, and Eimeria tenella oocysts as a vaccine against coccidiosis. Newly hatched chicks (Gallus gallus domesticus) were either sprayed with an aqueous suspension of Eimeria oocysts or were allowed to ingest feed containing Eimeria oocysts-incorporated gel-beads. Control day-old chicks were given an equivalent number of Eimeria oocysts (10(4) total) by oral gavage. After 3 days, chicks were randomly assigned to individual cages, and feces were collected between days 5 and 8 postinfection. All samples were processed for total Eimeria oocysts. At 4 wk of age, all chickens and a control nonimmunized group received a high-close E acervulina, E. maxima, and E. tenella challenge infection. Oocyst excretion by chicks fed gel-beads or inoculated by oral gavage was 10- to 100-fold greater than that of chicks spray-vaccinated with the Eimeria oocysts mixture (log 6.3-6.6 vs. log 4.8). Subsequent protection against challenge as measured by weight gain and feed conversion efficiency was significantly greater (P < 0.05) in gel-bead and oral gavage groups compared with spray-vaccinated or nonimmunized groups. Also, gel-bead and oral gavage groups showed no significant difference (P > 0.05) in weight gain and feed conversion efficiency compared with nonchallenged controls. These findings indicate that incorporation of Eimeria spp. oocysts in gel-beads may represent an effective way to deliver live oocyst vaccines to day-old chicks for preventing subsequent outbreaks of coccidiosis in the field. C1 [Jenkins, Mark C.; Parker, Carolyn; O'Brien, Celia; Miska, Katarzyna; Fetterer, Raymond] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Klopp, Spangler] Klopp Consulting Serv, Lewes, DE 19958 USA. RP Jenkins, MC (reprint author), ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM mark.jenkins@ars.usda.gov NR 19 TC 4 Z9 4 U1 1 U2 7 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 306 EP 309 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700007 PM 22856186 ER PT J AU Hines, NL Killian, ML Pedersen, JC Reising, MM Mosos, NA Mathieu-Benson, C Miller, CL AF Hines, Nichole L. Killian, Mary Lea Pedersen, Janice C. Reising, Monica M. Mosos, Nestor A. Mathieu-Benson, Christian Miller, Cathy L. TI An rRT-PCR Assay to Detect the Matrix Gene of a Broad Range of Avian Paramyxovirus Serotype-1 Strains SO AVIAN DISEASES LA English DT Article DE avian paramyxovirus serotype-1; Newcastle disease virus; rRT-PCR; matrix gene; pigeon paramyxovirus-1; National Veterinary Services Laboratories; National Animal Health Laboratory Network ID NEWCASTLE-DISEASE VIRUS; REVERSE-TRANSCRIPTION-PCR; CLASS-I AB The current U.S. Department of Agriculture (USDA) validated real-time reverse transcription polymerase chain reaction (rRT-PCR) assay designed to detect the matrix gene of avian paramyxovirus serotype-1 (APMV-1) is the primary screening assay used in the United States. It has previously been shown to be unable to consistently detect all members of class I APMV-1. Diagnostic testing relies on rRT-PCR to quickly detect APMV-1 in wild birds, backyard flocks, live bird markets, commercial poultry, and for export testing. Limitations of the current USDA assay have raised concerns about the potential for some strains of APMV-1 to remain undetected by the primary screening assay. Mismatches in the probe were shown to cause a loss in template binding efficiency, resulting in lack of detection by the assay. Here, we describe the development and analytical validation of a new rRT-PCR assay designed to target a highly conserved region of the matrix gene across a wide range of APMV-1 strains. Limit of detection testing revealed a 3 log(10) decrease in sensitivity for one low-virulence strain when compared to the USDA validated assay. Conversely, the assay showed increased sensitivity for a class I isolate and two virulent strains of APMV-1 that were not detected by the USDA-validated assay. The new assay also demonstrated a high degree of specificity by the lack of detection of 43 non APMV-1 viruses. C1 [Miller, Cathy L.] Iowa State Univ Sci & Technol, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. [Hines, Nichole L.; Killian, Mary Lea; Pedersen, Janice C.] Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, USDA, Ames, IA 50010 USA. [Mosos, Nestor A.] Inst Colombiano Agropecuario, Natl Vet Lab, Bogota, Colombia. [Mathieu-Benson, Christian] Serv Agr & Ganadero, Dept Labs Lo Aguirre, Santiago, Chile. RP Miller, CL (reprint author), Iowa State Univ Sci & Technol, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Ames, IA 50011 USA. EM clm@iastate.edu OI Miller, Cathy/0000-0002-2601-4422 NR 24 TC 6 Z9 6 U1 0 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 387 EP 395 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700020 PM 22856199 ER PT J AU Evans, JD Leigh, SA Purswell, JL Jacob, R Peebles, ED Collier, SD Branton, SL AF Evans, J. D. Leigh, S. A. Purswell, J. L. Jacob, R. Peebles, E. D. Collier, S. D. Branton, S. L. TI A Comparative Study of Live Attenuated F Strain-Derived Mycoplasma gallisepticum Vaccines SO AVIAN DISEASES LA English DT Article DE Mycoplasma gallisepticum; live vaccine; mycoplasmosis; F strain; AviPro MG F; Poulvac Myco F ID LAYER CHICKENS; SEROLOGIC RESPONSE; VACCINATION; MUTANT; TIME AB Commercially available attenuated strains of Mycoplasma gallisepticum (MG) are commonly used within the layer industry to control MG-induced mycoplasrnosis. Among these are two live MG vaccines derived from the moderately pathogenic MG "chick F" strain. In the present study, the commercially available F strain derivatives were compared for their ability to elicit seroconversion, persist in vivo, and protect against virulent MG-induced airsacculitis. In addition, a noncommercial laboratory derived high-passage F strain isolate was included in the study. Commercial (Hy-Line W-36) layers were placed in biological isolation units at 9 wk of age (woa). At 10 woa, birds within each biological isolation unit were treated via eye-drop application with one of the three F strain derived vaccines at one of four levels (1X, 10(-1)X, 10(-2)X, or 10(-3)X). For the commercially available F strain derivatives, IX equaled the manufacturer's recommended dose. The 1X dose of the noncommercial laboratory maintained F strain derivative equaled 20 mu l of a 48 hr culture. For wk 1-6 postvaccination (p.v.), sera were collected weekly from each bird, and seroconversion was assessed via serum plate agglutination (SPA). Virulent MG (strain R-low) challenge occurred via intratracheal inoculation at 7 wk p.v. Necropsies were subsequently performed to assess challenge-associated airsacculitus. For each F strain derivative applied at 1X and 10(-1)X, 100% seroconversion, as measured by SPA, was demonstrated by 6 wk p.v., and rates at the 10(-2)X dosage were 10% and 90% for the commercial vaccines and 60% for the laboratory-derived strain in this period. Following challenge, airsacculitis was observed in 66.67% of the nontreated controls but not in any 1X- or 10(-1)X-treated bird independent of applied F strain derivative. C1 [Evans, J. D.; Leigh, S. A.; Purswell, J. L.; Jacob, R.; Peebles, E. D.; Collier, S. D.; Branton, S. L.] ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. RP Evans, JD (reprint author), ARS, USDA, Poultry Res Unit, Mississippi State, MS 39762 USA. EM jeff.evans@ars.usda.gov NR 30 TC 9 Z9 10 U1 0 U2 10 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 396 EP 401 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700021 PM 22856200 ER PT J AU Lee, SH Lillehoj, HS Jang, SI Lee, KW Kim, DK Lillehoj, EP Yancey, RJ Dominowski, PJ AF Lee, Sung Hyen Lillehoj, Hyun S. Jang, Seung I. Lee, Kyung Woo Kim, Duk Kyung Lillehoj, Erik P. Yancey, Robert J. Dominowski, Paul J. TI Evaluation of Novel Adjuvant Eimeria Profilin Complex on Intestinal Host Immune Responses Against Live E. acervulina Challenge Infection SO AVIAN DISEASES LA English DT Article DE adjuvant; Eimeria; profilin; immunity; coccidiosis; broiler ID PROTECTIVE IMMUNITY; SALMONELLA-TYPHIMURIUM; BROILER-CHICKENS; TENELLA; VACCINATION; DNA; COCCIDIOSIS; VACCINES; PROTEIN; INDUCTION AB The effects against avian coccidiosis of two novel adjuvants, Quil A/cholesterol/dimethyl dioctadecyl ammonium bromide/Carbopol (QCDC) and QCDC/Bay R1005 (R)/cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides (CpG ODN rip (QCDCRT) emulsified with profilin, a conserved Eimeria recombinant protein, were determined in broiler chickens. Chickens were subcutaneously immunized with isotonic saline (control group), profilin (P), profilin emulsified with QCDC (P-Q), or profilin with QCDCRT (P-QR) at 2 and 9 days post-hatch and orally challenged with 1.0 X 10(4) sporulated oocysts of Eimeria acervulina (EA) at 7 days postimmunization. All profilin-immunized groups showed increased body weight gain when compared to the control group, and the P-QR group had significantly higher body weight gain than did those of the P and P-Q groups following EA challenge infection. All groups immunized with profilin showed significantly decreased intestinal lesions compared with the control group, with the P-QR group showing the lowest intestinal lesions among the profilin-treated groups. Finally, the P-QR group showed greater CD4+/CD8+ and TCR1(+)/TCR2+ splenocytes and higher antiprofilin serum antibody titers compared with the P and P-Q (or both) groups following EA challenge infection. These results further suggest that vaccination of chickens with profilin, in combination with the QCDCRT adjuvant, may provide a novel control strategy against EA infection in commercial flocks. C1 [Lee, Sung Hyen; Lillehoj, Hyun S.; Jang, Seung I.; Lee, Kyung Woo; Kim, Duk Kyung] ARS, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. [Lillehoj, Erik P.] Univ Maryland, Dept Pediat, Sch Med, Baltimore, MD 21201 USA. [Yancey, Robert J.; Dominowski, Paul J.] Pfizer Anim Hlth, Kalamazoo, MI 49007 USA. RP Lillehoj, HS (reprint author), ARS, Anim & Nat Resources Inst, USDA, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov OI Lee, Kyung-Woo/0000-0002-3533-7979 FU ARS CRIS [1265-32000-086] FX This project was partially supported by an ARS CRIS project 1265-32000-086 and a formal agreement established between ARS and Pfizer Animal Health. QCDC and QCDCRT were supplied by the Pfizer Inc (Kalamazoo, MI). The authors thank Margie Nichols and Stacy Torreyson for their significant contribution to this research. NR 27 TC 8 Z9 8 U1 1 U2 5 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2012 VL 56 IS 2 BP 402 EP 405 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA 965OS UT WOS:000305777700022 PM 22856201 ER PT J AU Perez-Figueroa, A Wallen, RL Antao, T Coombs, JA Schwartz, MK White, PJ Luikart, G AF Perez-Figueroa, Andres Wallen, Rick L. Antao, Tiago Coombs, Jason A. Schwartz, Michael K. White, P. J. Luikart, Gordon TI Conserving genomic variability in large mammals: Effect of population fluctuations and variance in male reproductive success on variability in Yellowstone bison SO BIOLOGICAL CONSERVATION LA English DT Article DE Allelic diversity; Effective population size; Simulation modelling; Age structure; Population growth rate; Population viability; Genomic diversity; Conservation genetics; Yellowstone bison ID ISLAND MOUFLON POPULATION; EFFECTIVE SIZE; OVERLAPPING GENERATIONS; GENETIC-VARIABILITY; AMERICAN BISON; MATING SYSTEM; LONG-TERM; CONSERVATION; DYNAMICS; HETEROZYGOSITY AB Loss of genetic variation through genetic drift can reduce population viability. However, relatively little is known about loss of variation caused by the combination of fluctuating population size and variance in reproductive success in age structured populations. We built an individual-based computer simulation model to examine how actual culling and hunting strategies influence the effective population size (N-e) and allelic diversity in Yellowstone bison over 200 years (similar to 28 generations). The N-e for simulated populations ranged from 746 in stable populations of size 2000 up to 1165 in fluctuating populations whose census size fluctuates between 3000 and 3500 individuals. Simulations suggested that similar to 93% of allelic diversity, for loci with five alleles will be maintained over 200 years if the population census size remains well above similar to 2000 bison (and if variance in male reproductive success is high). However for loci with 20 alleles. only 83% of allelic diversity will be maintained over 200 years. Removal of only juveniles (calves and yearlings) resulted in longer generation intervals which led to higher maintenance of allelic diversity (96%) after 200 years compared to the culling of adults (94%) when the mean census size was 3250 (for loci with five alleles). These simulations suggest that fluctuations in population census size do not necessarily accelerate the loss of genetic variation, at least for the relatively large census size and growing populations such as in Yellowstone bison. They also suggest that the conservation of high allelic diversity (>95%) at loci with many alleles (e.g., >= 5) will require maintenance of a populations size greater than approximately 3250 and removal of mainly or only juveniles. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Luikart, Gordon] Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA. [Perez-Figueroa, Andres] Univ Vigo, Fac Biol, Dept Bioquim Genet & Inmunol, Vigo 36310, Spain. [Perez-Figueroa, Andres; Luikart, Gordon] Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, CIBIO, P-4485661 Vairao, Portugal. [Wallen, Rick L.; White, P. J.] Natl Pk Serv, Yellowstone Natl Pk, WY 82190 USA. [Antao, Tiago] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England. [Coombs, Jason A.] Univ Massachusetts, Program Organism & Evolutionary Biol, Amherst, MA 01003 USA. [Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Luikart, Gordon] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. RP Luikart, G (reprint author), Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA. EM anpefi@uvigo.es; Rick_Wallen@nps.gov; Tiago.Antao@liverpool.ac.uk; jcoombs@cns.umass.edu; mkschwartz@fs.fed.us; PJ_White@nps.gov; gordon.luikart@mso.umt.edu RI Perez-Figueroa, Andres/A-7503-2008; Antao, Tiago/C-3615-2009; Schwartz, Michael/C-3184-2014; OI Perez-Figueroa, Andres/0000-0002-7722-3697; Antao, Tiago/0000-0002-7269-5513; Schwartz, Michael/0000-0003-3521-3367; Luikart, Gordon/0000-0001-8697-0582 FU National Park Service through the Rocky Mountain Cooperative Ecosystem Studies Unit at the University of Montana; Ministerio de Ciencia e Innovacion [CGL2009-13278-C02]; Xunta de Galicia; Fondos Feder (Grupos de Referencia Competitiva) [2010/80]; Angeles Alvarino fellowship from Xunta de Galicia (Spain); Portuguese Science Foundation (FCT) [SFRH/BD/30834/2006, PTDC/BIA-BDE/65625/2006]; Walton Family Foundation; CIBIO-UP; US National Park Service; US. National Science Foundation [DEB 1067613, DEB 074218] FX Funding for this work was provided by the National Park Service through the Rocky Mountain Cooperative Ecosystem Studies Unit at the University of Montana and by the Ministerio de Ciencia e Innovacion (CGL2009-13278-C02), Xunta de Galicia and Fondos Feder (Grupos de Referencia Competitiva, 2010/80). A.P.-F. was supported by an Angeles Alvarino fellowship from Xunta de Galicia (Spain). T.A. was supported by research grant SFRH/BD/30834/2006 from the Portuguese Science Foundation (FCT). G.L. was supported by grants from the Walton Family Foundation, CIBIO-UP, the US National Park Service, the Portuguese Science Foundation (FCT) grant PTDC/BIA-BDE/65625/2006, and the US. National Science Foundation Grants DEB 1067613 and DEB 074218. Helpful advice and comments were provided by A. Carvajal-Rodriguez, F. Gardipee, and three anonymous referees. We are especially grateful for the helpful advice and extensive revisions suggested by Fred Allendorf, such as more a careful and extensive discussion of culling effects on Ne and G (generation interval). NR 54 TC 2 Z9 2 U1 2 U2 59 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 J9 BIOL CONSERV JI Biol. Conserv. PD JUN PY 2012 VL 150 IS 1 BP 159 EP 166 DI 10.1016/j.biocon.2012.02.022 PG 8 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 964VF UT WOS:000305724100020 ER PT J AU Magri, A Vanotti, MB Szogi, AA AF Magri, Albert Vanotti, Matias B. Szoegi, Ariel A. TI Anammox sludge immobilized in polyvinyl alcohol (PVA) cryogel carriers SO BIORESOURCE TECHNOLOGY LA English DT Article DE Anaerobic ammonium oxidation (anammox); Immobilization; Gel cell entrapment; Nitrite inhibition; Swine wastewater ID ANAEROBIC AMMONIUM OXIDATION; ACCLIMATED NITRIFYING SLUDGE; SWINE WASTE-WATER; NITRIFICATION TREATMENT; REMOVAL PERFORMANCE; POLYETHYLENE-GLYCOL; OXIDIZING BACTERIA; NITROGEN REMOVAL; DIGESTER LIQUOR; REACTOR AB This study evaluated the use of PVA cryogels to encapsulate slow-growing anammox bacteria for deammonification treatment of wastewater. The cryogel pellets were prepared by freezing-thawing at -8 degrees C. On average, pellets contained 11.8 mg-TSS/g-pellet of enriched anammox sludge NRRL B-50286 (Candidatus Brocadia caroliniensis) in 4-mm cubes. They were tested with synthetic and partially nitrified swine wastewater using continuous stirred-tank reactors packed at 20% (w/v). The immobilized gel was retained inside the reactor by a screen that eliminated the need of sludge recycling. The stoichiometry of anammox reaction was maintained for more than 5 months under non-sterile conditions. The process was not limited by substrates availability unless quite low N concentration (< 5 mg/L) achieving > 93% removal efficiency. In mass balances, > 80% of the potential N conversion activity was achieved (2920 mg-N/kg-pellet/d). In addition, the immobilized bacteria were resilient to inhibition at high nitrite concentrations (244-270 mg-N/L). (C) 2012 Elsevier Ltd. All rights reserved. C1 [Magri, Albert; Vanotti, Matias B.; Szoegi, Ariel A.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Magri, Albert] GIRO Technol Ctr, Barcelona 08100, Spain. RP Magri, A (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 W Lucas Sr, Florence, SC 29501 USA. EM albert.magri@gmail.com; matias.vanotti@ars.usda.gov; ariel.szogi@ars.usda.gov RI Magri, Albert/B-5903-2008 OI Magri, Albert/0000-0002-6993-0746 FU USDA-ARS [214, 6657-13630-005-00D] FX This research was part of USDA-ARS National Program 214: Utilization of Manure and Other Agricultural and Industrial Byproducts, ARS Project 6657-13630-005-00D "Innovative Bioresource Management Technologies for Enhanced Environmental Quality and Value Optimization". First author thanks AGAUR - Generalitat de Catalunya (file 2009 BE2 00032) and USDA-ARS for funding his scientific visit to USDA Coastal Plains Soil, Water and Plant Research Center. The authors are grateful to Chris Brown for help with laboratory analyses. Mention of a specific product or vendor does not constitute a guarantee or warrant of the product by the USDA or imply its approval to the exclusion of other products that may be suitable. NR 35 TC 27 Z9 33 U1 5 U2 58 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JUN PY 2012 VL 114 BP 231 EP 240 DI 10.1016/j.biortech.2012.03.077 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 955QL UT WOS:000305035900031 PM 22520223 ER PT J AU Mischoulon, D Lamon-Fava, S Selhub, J Katz, J Papakostas, GI Iosifescu, DV Yeung, AS Dording, CM Farabaugh, AH Clain, AJ Baer, L Alpert, JE Nierenberg, AA Fava, M AF Mischoulon, David Lamon-Fava, Stefania Selhub, Jacob Katz, Judith Papakostas, George I. Iosifescu, Dan V. Yeung, Albert S. Dording, Christina M. Farabaugh, Amy H. Clain, Alisabet J. Baer, Lee Alpert, Jonathan E. Nierenberg, Andrew A. Fava, Maurizio TI Prevalence of MTHFR C677T and MS A2756G polymorphisms in major depressive disorder, and their impact on response to fluoxetine treatment SO CNS SPECTRUMS LA English DT Article DE Methylene Tetrahydrofolate Reductase; MTHFR; Methionine Synthase; MS; C677T; A2756G; depression; antidepressant; fluoxetine ID METHYLENETETRAHYDROFOLATE REDUCTASE VARIANT; CORONARY-ARTERY-DISEASE; METHIONINE SYNTHASE; FOLIC-ACID; HOMOCYSTEINE LEVELS; GENE POLYMORPHISM; SERUM FOLATE; INDIAN POPULATION; RISK-FACTORS; EARLY-ONSET AB Objective. To examine the prevalence of the C677T polymorphism of the methylene tetrahydrofolate reductase (MTHFR) gene and the A2756G polymorphism of methionine synthase (MS), and their impact on antidepressant response. Methods. We screened 224 subjects (52% female, mean age 39 +/- 11 years) with SCID-diagnosed major depressive disorder (MDD), and obtained 194 genetic samples. 49 subjects (49% female, mean age 36 +/- 11 years) participated in a 12-week open clinical trial of fluoxetine 20-60 mg/day. Association between clinical response and C677T and A2756G polymorphisms, folate, B12, and homocysteine was examined. Results. Prevalence of the C677T and A2756G polymorphisms was consistent with previous reports (C/C = 41%, C/T = 47%, T/T = 11%, A/A = 66%, A/G = 29%, G/G = 4%). In the fluoxetine-treated subsample (n=49), intent-to-treat (ITT) response rates were 47% for C/C subjects and 46% for pooled C/T and T/T subjects (nonsignificant). ITT response rates were 38% for A/A subjects and 60% for A/G subjects (nonsignificant), with no subjects exhibiting the G/G homozygote. Mean baseline plasma B12 was significantly lower in A/G subjects compared to A/A, but folate and homocysteine levels were not affected by genetic status. Plasma folate was negatively associated with treatment response. Conclusion. The C677T and A2756G polymorphisms did not significantly affect antidepressant response. These preliminary findings require replication in larger samples. C1 [Mischoulon, David; Katz, Judith; Papakostas, George I.; Iosifescu, Dan V.; Yeung, Albert S.; Dording, Christina M.; Farabaugh, Amy H.; Clain, Alisabet J.; Baer, Lee; Alpert, Jonathan E.; Nierenberg, Andrew A.; Fava, Maurizio] Massachusetts Gen Hosp, Depress Clin & Res Program, Boston, MA 02114 USA. [Mischoulon, David; Katz, Judith; Papakostas, George I.; Iosifescu, Dan V.; Yeung, Albert S.; Dording, Christina M.; Farabaugh, Amy H.; Clain, Alisabet J.; Baer, Lee; Alpert, Jonathan E.; Nierenberg, Andrew A.; Fava, Maurizio] Harvard Univ, Sch Med, Boston, MA USA. [Lamon-Fava, Stefania; Selhub, Jacob] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Mischoulon, D (reprint author), Massachusetts Gen Hosp, Depress Clin & Res Program, 1 Bowdoin Sq,6th Floor, Boston, MA 02114 USA. EM dmischoulon@partners.org RI Papakostas, George/I-6905-2013; OI Papakostas, George/0000-0002-2465-5103; Alpert, Jonathan/0000-0002-4332-908X FU National Association for Research on Schizophrenia and Depression (NARSAD); NIMH [R10-MH56058]; State of New York; Nordic Naturals; Fisher Wallace; Bowman Family Foundation; Ganeden; Back Bay Scientific; AstraZeneca PLC; Bristol-Myers Squibb Company; Forest Pharmaceuticals; National Institute of Mental Health; PAMLAB LLC; Pfizer Inc.; Ridge Diagnostics; Aspect Medical Systems; Forest Laboratories; Janssen Pharmaceutica; Eli Lilly; Pamlab; Pfizer; American Society of Clinical Psychopharmacology; Australasian Society for Bipolar Disorder; Bayamon Region Psychiatric Society; San Juan; Belvoir Publishing; CRICO; Dartmouth; Dey Pharma; L. P./Mylan Inc.; Israel Society for Biological Psychiatry; John Hopkins University; National Association of Continuing Education; Physicians Postgraduate Press; Slack Publishing; University of Florida; Alkermes; Astra-Zeneca; BioResearch; BrainCells, Inc.; Cephalon; Clinical Trial Solutions, LLC; Eli Lilly Company; EnVivo Pharmaceuticals, Inc.; Forest Pharmaceuticals Inc.; GlaxoSmithKline; J & J Pharmaceuticals; Lichtwer Pharma GmbH; Lorex Pharmaceuticals; NARSAD; NCCAM; NIDA; Novartis; Organon Inc.; PamLab, LLC; Pfizer Inc; Pharmavite; Roche; Sanofi-Aventis; Shire; Solvay Pharmaceuticals, Inc.; Synthelabo; Wyeth-Ayerst Laboratories FX Dr. David Mischoulon was supported in this investigation by a Young Investigator Award from the National Association for Research on Schizophrenia and Depression (NARSAD).; The fluoxetine parent study was supported by NIMH grant R10-MH56058 to Dr. Patrick McGrath and by infrastructure and salary support from the State of New York. The authors thank Dr. McGrath and his colleagues at Columbia University College of Physicians and Surgeons and the New York State Psychiatric Institute, New York.; Dr. David Mischoulon has received research support from Nordic Naturals, Fisher Wallace, the Bowman Family Foundation, and Ganeden. He has served as a consultant to Bristol-Meyers-Squibb Company. He has received writing honoraria from Pamlab and Nordic Naturals and speaking honoraria from Nordic Naturals. He has received royalties from Back Bay Scientific for PMS Escape, and royalties from Lippincott Williams & Wilkins, for textbook "Natural Medications for Psychiatric Disorders: Considering the Alternatives'' (David Mischoulon and Jerrold F Rosenbaum, Eds.).; Dr. George Papakostas has served as a consultant for Abbott Laboratories, AstraZeneca PLC, Brainsway Ltd, Bristol-Myers Squibb Company, Cephalon Inc., Dey Pharma, L. P., Eli Lilly Co., GlaxoSmithKline, Evotec AG, Inflabloc Pharmaceuticals, Jazz Pharmaceuticals, Otsuka Pharmaceuticals, PAMLAB LLC, Pfizer Inc., Pierre Fabre Laboratories, Ridge Diagnostics (formerly known as Precision Human Biolaboratories), Shire Pharmaceuticals, Takeda Pharmaceutical Company LTD, Theracos, Inc., and Wyeth, Inc. He has received honoraria from Abbott Laboratories, Astra Zeneca PLC, Bristol-Myers Squibb Company, Brainsway Ltd, Cephalon Inc., Dey Pharma, L. P., Eli Lilly Co., Evotec AG, GlaxoSmithKline, Inflabloc Pharmaceuticals, Jazz Pharmaceuticals, Lundbeck, Otsuka Pharmaceuticals, PAMLAB LLC, Pfizer, Pierre Fabre Laboratories, Ridge Diagnostics, Shire Pharmaceuticals, Takeda Pharmaceutical Company LTD, Theracos, Inc., Titan Pharmaceuticals, and Wyeth Inc. He has received research support from AstraZeneca PLC, Bristol-Myers Squibb Company, Forest Pharmaceuticals, the National Institute of Mental Health, PAMLAB LLC, Pfizer Inc., and Ridge Diagnostics (formerly known as Precision Human Biolaboratories). He has served (not currently) on the speaker's bureau for BristolMyersSquibb Co and Pfizer, Inc.; Dr. Daniel Iosifescu has received research support from Aspect Medical Systems, Forest Laboratories and Janssen Pharmaceutica; he has been a consultant for Forest Laboratories, Gerson Lehrman Group and Pfizer, Inc., and he has been a speaker for Cephalon, Inc., Eli Lilly & Co., Forest Laboratories, and Pfizer, Inc.; Dr. Jonathan Alpert has received research support from Aspect Medical Systems, Eli Lilly, Pamlab, and Pfizer; he has received speaker's honoraria from Reed Medical Education and Belvoir Publishing.; Dr. Andrew Nierenberg has received honoraria or travel expenses from: American Society of Clinical Psychopharmacology, Australasian Society for Bipolar Disorder, Bayamon Region Psychiatric Society, San Juan, Puerto Rico, Belvoir Publishing, CRICO, Dartmouth, Dey Pharma, L. P./Mylan Inc., Israel Society for Biological Psychiatry, John Hopkins University, National Association of Continuing Education, Physicians Postgraduate Press, Slack Publishing, University of Florida, University of Michigan, University of Miami, Wolters Klower Publishing. Dr. Nierenberg owns stock options in Appliance Computing, Inc. (MindSite.com) and Brain Cells, Inc. Additional income is possible from Infomedic.com depending on overall revenues of the company but no revenue has been received to date. Through MGH, Dr. Nierenberg is named for copyrights to: the Clinical Positive Affect Scale and the MGH Structured Clinical Interview for the Montgomery Asberg Depression Scale exclusively licensed to the MGH Clinical Trials Network and Institute (CTNI).; Dr. Maurizio Fava has received research support from Abbott Laboratories, Alkermes, Aspect Medical Systems, Astra-Zeneca, BioResearch, BrainCells, Inc., Bristol-Myers Squibb Company, Cephalon, Clinical Trial Solutions, LLC, Eli Lilly & Company, EnVivo Pharmaceuticals, Inc., Forest Pharmaceuticals Inc., Ganeden, GlaxoSmithKline, J & J Pharmaceuticals, Lichtwer Pharma GmbH, Lorex Pharmaceuticals, NARSAD, NCCAM, NIDA, NIMH, Novartis, Organon Inc., PamLab, LLC, Pfizer Inc, Pharmavite, Roche, Sanofi-Aventis, Shire, Solvay Pharmaceuticals, Inc., Synthelabo, and Wyeth-Ayerst Laboratories. He has served as an advisor and consultant to Abbott Laboratories, Affectis Pharmaceuticals AG, Amarin, Aspect Medical Systems, Astra-Zeneca, Auspex Pharmaceuticals, Bayer AG, Best Practice Project Management, Inc, BioMarin Pharmaceuticals, Inc., Biovail Pharmaceuticals, Inc., BrainCells, Inc, Bristol-Myers Squibb Company, Cephalon, Clinical Trials Solutions, LLC, CNS Response, Compellis, Cypress Pharmaceuticals, Dov Pharmaceuticals, Eisai, Inc., Eli Lilly & Company, EPIX Pharmaceuticals, Euthymics Bioscience, Inc., Fabre-Kramer, Pharmaceuticals, Inc., Forest Pharmaceuticals Inc., GlaxoSmithKline, Grunenthal GmBH, Janssen Pharmaceutica, Jazz Pharmaceuticals, J & J Pharmaceuticals, Knoll Pharmaceutical Company, Labopharm, Lorex Pharmaceuticals, Lundbeck, MedAvante Inc., Merck, Methylation Sciences, Neuronetics, Novartis, Nutrition 21, Organon Inc., PamLab, LLC, Pfizer Inc, PharmaStar, Pharmavite, Precision Human Biolaboratory, Prexa Pharmaceuticals, Inc., PsychoGenics, Psylin Neurosciences, Inc., Ridge Diagnostics, Inc., Roche, Sanofi-Aventis, Sepracor, Schering-Plough, Solvay Pharmaceuticals, Inc., Somaxon, Somerset Pharmaceuticals, Synthelabo, Takeda, Tetragenex, TransForm Pharmaceuticals, Inc., Transcept Pharmaceuticals, Vanda Pharmaceuticals Inc, Wyeth-Ayerst Laboratories. He has received speaking and publishing honoraria from Adamed, Co., Advanced Meeting Partners, American Psychiatric Association, American Society of Clinical Psychopharmacology, Astra-Zeneca, Belvoir, Boehringer-Ingelheim, Bristol-Myers Squibb Company, Cephalon, Eli Lilly & Company, Forest Pharmaceuticals Inc., GlaxoSmithKline, Imedex, Novartis, Organon Inc., Pfizer Inc, PharmaStar, MGH Psychiatry Academy/Primedia, MGH Psychiatry Academy/Reed-Elsevier, UBC, and Wyeth-Ayerst Laboratories. He holds equity in Compellis. He currently holds a patent for SPCD and a patent application for a combination of azapirones and bupropion in MDD, and has received copyright royalties for the MGH CPFQ, SFI, ATRQ, DESS, and SAFER diagnostic instruments. NR 59 TC 5 Z9 6 U1 2 U2 12 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 1092-8529 J9 CNS SPECTRUMS JI CNS Spectr. PD JUN PY 2012 VL 17 IS 2 BP 76 EP 86 DI 10.1017/S1092852912000430 PG 11 WC Clinical Neurology; Psychiatry SC Neurosciences & Neurology; Psychiatry GA 967LL UT WOS:000305909200003 PM 22789065 ER PT J AU Steen, DA McClure, CJW Brock, JC Rudolph, DC Pierce, JB Lee, JR Humphries, WJ Gregory, BB Sutton, WB Smith, LL Baxley, DL Stevenson, DJ Guyer, C AF Steen, David A. McClure, Christopher J. W. Brock, Jean C. Rudolph, D. Craig Pierce, Josh B. Lee, James R. Humphries, W. Jeffrey Gregory, Beau B. Sutton, William B. Smith, Lora L. Baxley, Danna L. Stevenson, Dirk J. Guyer, Craig TI Landscape-level influences of terrestrial snake occupancy within the southeastern United States SO ECOLOGICAL APPLICATIONS LA English DT Article DE Agkistrodon contortrix; Coluber spp.; Crotalus spp.; detection probability; Elaphe spp.; Heterodon platirhinos; Lampropeltis getula; landscape ecology; occupancy modeling; Pituophis spp.; squamate reptile; Thamnophis sirtalis ID KINGSNAKE LAMPROPELTIS-GETULA; MULTISCALE HABITAT SELECTION; LOUISIANA PINE SNAKE; GULF COASTAL-PLAIN; BLACK RAT SNAKES; ELAPHE-OBSOLETA; SPATIAL ECOLOGY; MASTICOPHIS-FLAGELLUM; LONGLEAF PINE; EASTERN TEXAS AB Habitat loss and degradation are thought to be the primary drivers of species extirpations, but for many species we have little information regarding specific habitats that influence occupancy. Snakes are of conservation concern throughout North America, but effective management and conservation are hindered by a lack of basic natural history information and the small number of large-scale studies designed to assess general population trends. To address this information gap, we compiled detection/nondetection data for 13 large terrestrial species from 449 traps located across the southeastern United States, and we characterized the land cover surrounding each trap at multiple spatial scales (250-, 500-, and 1000-m buffers). We used occupancy modeling, while accounting for heterogeneity in detection probability, to identify habitat variables that were influential in determining the presence of a particular species. We evaluated 12 competing models for each species, representing various hypotheses pertaining to important habitat features for terrestrial snakes. Overall, considerable interspecific variation existed in important habitat variables and relevant spatial scales. For example, kingsnakes (Lampropeltis getula) were negatively associated with evergreen forests, whereas Louisiana pinesnake (Pituophis ruthveni) occupancy increased with increasing coverage of this forest type. Some species were positively associated with grassland and scrub/shrub (e.g., Slowinski's cornsnake, Elaphe slowinskii) whereas others, (e. g., copperhead, Agkistrodon contortrix, and eastern diamond-backed rattlesnake, Crotalus adamanteus) were positively associated with forested habitats. Although the species that we studied may persist in varied landscapes other than those we identified as important, our data were collected in relatively undeveloped areas. Thus, our findings may be relevant when generating conservation plans or restoration goals. Maintaining or restoring landscapes that are most consistent with the ancestral habitat preferences of terrestrial snake assemblages will require a diverse habitat matrix over large spatial scales. C1 [Steen, David A.; Brock, Jean C.; Smith, Lora L.] Joseph W Jones Ecol Res Ctr, Newton, GA 39870 USA. [Steen, David A.; McClure, Christopher J. W.; Guyer, Craig] Auburn Univ, Dept Biol Sci, Auburn, AL 36849 USA. [Rudolph, D. Craig; Pierce, Josh B.] US Forest Serv, So Res Stn, Nacogdoches, TX 75965 USA. [Lee, James R.] Nature Conservancy, Camp Shelby, MS 39407 USA. [Humphries, W. Jeffrey] N Carolina Wildlife Resources Commiss, Chapel Hill, NC 27516 USA. [Gregory, Beau B.] Louisiana Dept Wildlife & Fisheries, Coastal & Nongame Resources Div, Baton Rouge, LA 70808 USA. [Sutton, William B.] Univ Tennessee, Dept Forestry Wildlife & Fisheries, Knoxville, TN 37996 USA. [Baxley, Danna L.] Kentucky Dept Fish & Wildlife Resources, Frankfort, KY 40601 USA. [Stevenson, Dirk J.] Orianne Soc, Indigo Snake Initiat, Clayton, GA 30525, Australia. RP Steen, DA (reprint author), Joseph W Jones Ecol Res Ctr, 3988 Jones Ctr Dr, Newton, GA 39870 USA. EM davidasteen@gmail.com OI McClure, Christopher/0000-0003-1216-7425 FU Department of Defense's Strategic Environmental Research and Development Program (SERDP) [SI-1696]; Florida Fish and Wildlife Conservation Commission's Wildlife Legacy Initiative program; U.S. Fish and Wildlife Service's State Wildlife Grants program [SWG 05-020, 060010]; University of Florida; Joseph W. Jones Ecological Research Center; Mississippi Army National Guard; DeSoto National Forest; MDWFP; MNHP; USFWS FX Funding for the Eglin Air Force Base study was provided by the Department of Defense's Strategic Environmental Research and Development Program (SERDP) Project Number: SI-1696. The Jones Center project was partially funded by the Florida Fish and Wildlife Conservation Commission's Wildlife Legacy Initiative program and the U.S. Fish and Wildlife Service's State Wildlife Grants program (Grant #SWG 05-020, Agreement #060010). Matching funds were provided by the University of Florida and Joseph W. Jones Ecological Research Center. Work at Camp Shelby would not have been possible without the financial support of the Mississippi Army National Guard, and collaboration with the DeSoto National Forest, MDWFP, MNHP, and the USFWS. We thank the many field technicians who provided assistance checking and maintaining traps for all the projects. M. Simmons provided assistance managing the land cover data. E. P. Cox (Jones Center) and Auburn University librarians provided assistance obtaining references. K. Barrett, A. M. Durso, J. Bauder, and O. Sylvester provided helpful comments on earlier drafts of the manuscript. NR 110 TC 21 Z9 21 U1 5 U2 81 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2012 VL 22 IS 4 BP 1084 EP 1097 PG 14 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 966KN UT WOS:000305836600003 PM 22827120 ER PT J AU Rodhouse, TJ Ormsbee, PC Irvine, KM Vierling, LA Szewczak, JM Vierling, KT AF Rodhouse, Thomas J. Ormsbee, Patricia C. Irvine, Kathryn M. Vierling, Lee A. Szewczak, Joseph M. Vierling, Kerri T. TI Assessing the status and trend of bat populations across broad geographic regions with dynamic distribution models SO ECOLOGICAL APPLICATIONS LA English DT Article DE bats; Bayesian hierarchical model; Chiroptera; conservation; detectability; monitoring; Myotis lucifugus; net primary productivity; occupancy; species distribution; species-energy theory; trend ID WHITE-NOSE SYNDROME; SPECIES DISTRIBUTION MODELS; MYOTIS-LUCIFUGUS; FIELD IDENTIFICATION; SPATIAL-DISTRIBUTION; WATER AVAILABILITY; PACIFIC-NORTHWEST; OCCUPANCY MODELS; SITE-OCCUPANCY; UNITED-STATES AB Bats face unprecedented threats from habitat loss, climate change, disease, and wind power development, and populations of many species are in decline. A better ability to quantify bat population status and trend is urgently needed in order to develop effective conservation strategies. We used a Bayesian autoregressive approach to develop dynamic distribution models for Myotis lucifugus, the little brown bat, across a large portion of northwestern USA, using a four-year detection history matrix obtained from a regional monitoring program. This widespread and abundant species has experienced precipitous local population declines in northeastern USA resulting from the novel disease white-nose syndrome, and is facing likely range-wide declines. Our models were temporally dynamic and accounted for imperfect detection. Drawing on species-energy theory, we included measures of net primary productivity (NPP) and forest cover in models, predicting that M. lucifugus occurrence probabilities would covary positively along those gradients. Despite its common status, M. lucifugus was only detected during similar to 50% of the surveys in occupied sample units. The overall naive estimate for the proportion of the study region occupied by the species was 0.69, but after accounting for imperfect detection, this increased to similar to 0.90. Our models provide evidence of an association between NPP and forest cover and M. lucifugus distribution, with implications for the projected effects of accelerated climate change in the region, which include net aridification as snowpack and stream flows decline. Annual turnover, the probability that an occupied sample unit was a newly occupied one, was estimated to be low (similar to 0.04-0.14), resulting in flat trend estimated with relatively high precision (SD = 0.04). We mapped the variation in predicted occurrence probabilities and corresponding prediction uncertainty along the productivity gradient. Our results provide a much needed baseline against which future anticipated declines in M. lucifugus occurrence can be measured. The dynamic distribution modeling approach has broad applicability to regional bat monitoring efforts now underway in several countries and we suggest ways to improve and expand our grid-based monitoring program to gain robust insights into bat population status and trend across large portions of North America. C1 [Rodhouse, Thomas J.; Vierling, Kerri T.] Univ Idaho, Dept Fish & Wildlife, Moscow, ID 83844 USA. [Ormsbee, Patricia C.] US Forest Serv, Willamette Natl Forest, Eugene, OR 97401 USA. [Irvine, Kathryn M.] US Geol Survey, No Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. [Vierling, Lee A.] Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA. [Szewczak, Joseph M.] Humboldt State Univ, Dept Biol Sci, Arcata, CA 95521 USA. RP Rodhouse, TJ (reprint author), Upper Columbia Basin Network, Natl Pk Serv, 63095 Deschutes Market Rd, Bend, OR 97701 USA. EM Tom_Rodhouse@nps.gov RI Vierling, Lee/E-6428-2010; Vierling, Kerri/N-6653-2016; OI Vierling, Lee/0000-0001-5344-1983; Rodhouse, Thomas/0000-0001-5953-9113 FU U.S. Forest Service; Bureau of Land Management; Department of Defense; National Park Service Upper Columbia Basin Network FX Funding for the Bat Grid was provided by the U.S. Forest Service, Bureau of Land Management, and Department of Defense Legacy Program. The National Park Service Upper Columbia Basin Network provided additional funding and support. We thank all the contributors to the Bat Grid: Without their extraordinary effort this analysis would not have been possible. Lew Cousineau provided invaluable data management support. Jeremy Hobson provided GIS support. Gerry Wright provided helpful comments during the preparation of the manuscript. The comments and suggestions provided by two anonymous reviewers greatly improved the manuscript. This is contribution 1070 of the University of Idaho Forest, Wildlife and Range Experiment Station. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 88 TC 12 Z9 13 U1 3 U2 103 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2012 VL 22 IS 4 BP 1098 EP 1113 PG 16 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 966KN UT WOS:000305836600004 PM 22827121 ER PT J AU Sturtevant, BR Miranda, BR Shinneman, DJ Gustafson, EJ Wolter, PT AF Sturtevant, Brian R. Miranda, Brian R. Shinneman, Douglas J. Gustafson, Eric J. Wolter, Peter T. TI Comparing modern and presettlement forest dynamics of a subboreal wilderness: Does spruce budworm enhance fire risk? SO ECOLOGICAL APPLICATIONS LA English DT Article DE Abies balsamea; disturbance ecology; insect-fire interactions; LANDIS-II; Laurentian mixed forest; Minnesota; spruce budworm; succession ID SOUTHERN-BOREAL FOREST; SUB-ALPINE FORESTS; BALSAM FIR; CHORISTONEURA-FUMIFERANA; EASTERN CANADA; SPATIAL-DISTRIBUTION; NORTHERN MINNESOTA; NEW-BRUNSWICK; LANDSCAPE; OUTBREAK AB Insect disturbance is often thought to increase fire risk through enhanced fuel loadings, particularly in coniferous forest ecosystems. Yet insect disturbances also affect successional pathways and landscape structure that interact with fire disturbances (and vice-versa) over longer time scales. We applied a landscape succession and disturbance model (LANDIS-II) to evaluate the relative strength of interactions between spruce budworm (Choristoneura fumiferana) outbreaks and fire disturbances in the Boundary Waters Canoe Area (BWCA) in northern Minnesota (USA). Disturbance interactions were evaluated for two different scenarios: presettlement forests and fire regimes vs. contemporary forests and fire regimes. Forest composition under the contemporary scenario trended toward mixtures of deciduous species (primarily Betula papyrifera and Populus spp.) and shade-tolerant conifers (Picea mariana, Abies balsamea, Thuja occidentalis), with disturbances dominated by a combination of budworm defoliation and high-severity fires. The presettlement scenario retained comparatively more "big pines" (i.e., Pinus strobus, P. resinosa) and tamarack (L. laricina), and experienced less budworm disturbance and a comparatively less-severe fire regime. Spruce budworm disturbance decreased area burned and fire severity under both scenarios when averaged across the entire 300-year simulations. Contrary to past research, area burned and fire severity during outbreak decades were each similar to that observed in non-outbreak decades. Our analyses suggest budworm disturbances within forests of the BWCA have a comparatively weak effect on long-term forest composition due to a combination of characteristics. These include strict host specificity, fine-scaled patchiness created by defoliation damage, and advance regeneration of its primary host, balsam fir (A. balsamea) that allows its host to persist despite repeated disturbances. Understanding the nature of the three-way interaction between budworm, fire, and composition has important ramifications for both fire mitigation strategies and ecosystem restoration initiatives. We conclude that budworm disturbance can partially mitigate long-term future fire risk by periodically reducing live ladder fuel within the mixed forest types of the BWCA but will do little to reverse the compositional trends caused in part by reduced fire rotations. C1 [Sturtevant, Brian R.; Miranda, Brian R.; Gustafson, Eric J.] USDA Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, Rhinelander, WI 54501 USA. [Shinneman, Douglas J.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Boise, ID 83706 USA. [Wolter, Peter T.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. RP Sturtevant, BR (reprint author), USDA Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, Rhinelander, WI 54501 USA. EM bsturtevant@fs.fed.us FU U.S. National Fire Plan; USDA Cooperative State Research, Education, and Extension Service (CSREES) Managed Ecosystems program [2005-35101-16342] FX This research was supported by the U.S. National Fire Plan and the USDA Cooperative State Research, Education, and Extension Service (CSREES) Managed Ecosystems program (2005-35101-16342). We thank Sue Lietz for her modeling and technical support and Robert Scheller for technical assistance with LANDIS-II. John Stanovick provided valuable guidance on our statistical methods. We also thank Daniel Kneeshaw, Guillaume Sainte-Marie, Kasey Legaard, Erin Simons, and two anonymous reviewers for helpful comments on the manuscript. NR 115 TC 8 Z9 8 U1 3 U2 50 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2012 VL 22 IS 4 BP 1278 EP 1296 PG 19 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 966KN UT WOS:000305836600018 PM 22827135 ER PT J AU Powers, MD Kolka, RK Bradford, JB Palik, BJ Fraver, S Jurgensen, MF AF Powers, Matthew D. Kolka, Randall K. Bradford, John B. Palik, Brian J. Fraver, Shawn Jurgensen, Martin F. TI Carbon stocks across a chronosequence of thinned and unmanaged red pine (Pinus resinosa) stands SO ECOLOGICAL APPLICATIONS LA English DT Article DE carbon dynamics; carbon storage; Chippewa National Forest, Minnesota, USA; forest management; Pinus resinosa; red pine; stand age; thinning ID COARSE WOODY DEBRIS; FLOOR ORGANIC-MATTER; SOIL CARBON; ECOSYSTEM CARBON; WESTERN OREGON; PONDEROSA PINE; FOREST CARBON; DISTURBANCE HISTORY; AGE-SEQUENCE; DOUGLAS-FIR AB Forests function as a major global C sink, and forest management strategies that maximize C stocks offer one possible means of mitigating the impacts of increasing anthropogenic CO2 emissions. We studied the effects of thinning, a common management technique in many forest types, on age-related trends in C stocks using a chronosequence of thinned and unmanaged red pine (Pinus resinosa) stands ranging from 9 to 306 years old. Live tree C stocks increased with age to a maximum near the middle of the chronosequence in unmanaged stands, and increased across the entire chronosequence in thinned stands. C in live understory vegetation and C in the mineral soil each declined rapidly with age in young stands but changed relatively little in middle-aged to older stands regardless of management. Forest floor C stocks increased with age in unmanaged stands, but forest floor C decreased with age after the onset of thinning around age 40 in thinned stands. Deadwood C was highly variable, but decreased with age in thinned stands. Total ecosystem C increased with stand age until approaching an asymptote around age 150. The increase in total ecosystem C was paralleled by an age-related increase in total aboveground C, but relatively little change in total belowground C. Thinning had surprisingly little impact on total ecosystem C stocks, but it did modestly alter age-related trends in total ecosystem C allocation between aboveground and belowground pools. In addition to characterizing the subtle differences in C dynamics between thinned and unmanaged stands, these results suggest that C accrual in red pine stands continues well beyond the 60-100 year management rotations typical for this system. Management plans that incorporate longer rotations and thinning in some stands could play an important role in maximizing C stocks in red pine forests while meeting other objectives including timber extraction, biodiversity conservation, restoration, and fuel reduction goals. C1 [Powers, Matthew D.; Jurgensen, Martin F.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Powers, Matthew D.; Kolka, Randall K.; Bradford, John B.; Palik, Brian J.; Fraver, Shawn] USDA Forest Serv, No Res Stn, Grand Rapids, MN 55744 USA. RP Powers, MD (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, 1400 Townsend Dr, Houghton, MI 49931 USA. EM mdpowers@mtu.edu RI Bradford, John/E-5545-2011 FU USDA Forest Service Northern Research Station; Agenda 2020 Program FX We thank Nate Aspelin, Doug Kastendick, Eric Doty, Ryan Kolka, and the staff of the Chippewa National Forest for their assistance with stand selection, data collection, and sample processing. Tony D'Amato, Christel Kern, and two anonymous reviewers provided valuable comments on earlier versions of the manuscript. Funding for this project was provided by the USDA Forest Service Northern Research Station and the Agenda 2020 Program. NR 67 TC 15 Z9 16 U1 1 U2 48 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2012 VL 22 IS 4 BP 1297 EP 1307 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 966KN UT WOS:000305836600019 PM 22827136 ER PT J AU Rinella, MJ Mangold, JM Espeland, EK Sheley, RL Jacobs, JS AF Rinella, Matthew J. Mangold, Jane M. Espeland, Erin K. Sheley, Roger L. Jacobs, James S. TI Long-term population dynamics of seeded plants in invaded grasslands SO ECOLOGICAL APPLICATIONS LA English DT Article DE Centaurea maculosa; grassland; herbicide; invasive weed; rehabilitation; reintroduction; revegetation; seeding ID KNAPWEED INFESTED RANGELAND; EUPHORBIA-ESULA CONTROL; INVASIVE WEED; DOMINATED RANGELAND; SEEDING TREATMENTS; ACROPTILON-REPENS; SINGLE ENTRY; P-VALUES; ESTABLISHMENT; REVEGETATION AB In recent decades, dozens of studies have involved attempts to introduce native and desirable nonnative plant species into grasslands dominated by invasive weeds. The newly introduced plants have proved capable of establishing, but because they are rarely monitored for more than four years, it is unknown if they have a high likelihood of persisting and suppressing invaders for the long term. Beyond invaded grasslands, this lack of long-term monitoring is a general problem plaguing efforts to reintroduce a range of taxa into a range of ecosystems. We introduced species from seed and then periodically measured plant abundances for nine years at one site and 15 years at a second site. To our knowledge, our 15-year data are the longest to date from a seeding experiment in invaded, never-cultivated grassland. At one site, three seeded grasses maintained high densities for three or more years, but then all or nearly all individuals died. At the second site, one grass performed similarly, but two other grasses proliferated and at least one greatly suppressed the dominant invader (Centaurea maculosa). In one study, our point estimate suggests that the seeded grass Thinopyrum intermedium reduced C. maculosa biomass by 93% 15 years after seeding. In some cases, data from three and fewer years after seeding falsely suggested that seeded species were capable of persisting within the invaded grassland. In other cases, data from as late as nine years after seeding falsely suggested seeded populations would not become large enough to suppress the invader. These results show that seeded species sometimes persist and suppress invaders for long periods, but short-term data cannot predict if, when, or where this will occur. Because short-term data are not predictive of long-term seeded species performances, additional long-term data are needed to identify effective practices, traits, and species for revegetating invaded grasslands. C1 [Rinella, Matthew J.] USDA ARS, Miles City, MT 59301 USA. [Mangold, Jane M.] Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Espeland, Erin K.] USDA ARS, Sidney, MT 59270 USA. [Sheley, Roger L.] USDA ARS, Burns, OR 97720 USA. [Jacobs, James S.] Nat Resources Conservat Serv, Bozeman, MT 59715 USA. RP Rinella, MJ (reprint author), USDA ARS, 243 Ft Keogh Rd, Miles City, MT 59301 USA. EM Matt.rinella@ars.usda.gov NR 57 TC 7 Z9 10 U1 4 U2 37 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 J9 ECOL APPL JI Ecol. Appl. PD JUN PY 2012 VL 22 IS 4 BP 1320 EP 1329 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 966KN UT WOS:000305836600021 PM 22827138 ER PT J AU Luo, W Pietravalle, S Parnell, S van den Bosch, F Gottwald, TR Trey, MS Parker, SR AF Luo, W. Pietravalle, S. Parnell, S. van den Bosch, F. Gottwald, T. R. Trey, M. S. Parker, S. R. TI An improved regulatory sampling method for mapping and representing plant disease from a limited number of samples SO EPIDEMICS LA English DT Article DE Dispersal gradients; Disease spread; Huanglongbing disease; Indicator kriging; Strawberry powdery mildew; Uncertainty analysis ID GEOGRAPHIC INFORMATION-SYSTEMS; DISPERSAL GRADIENT; STOCHASTIC-MODELS; SPORE DISPERSAL; POWDERY MILDEW; SPREAD; STRAWBERRY; GEOSTATISTICS; EPIDEMICS; PATHOGENS AB A key challenge for plant pathologists is to develop efficient methods to describe spatial patterns of disease spread accurately from a limited number of samples. Knowledge of disease spread is essential for informing and justifying plant disease management measures. A mechanistic modelling approach is adopted for disease mapping which is based on disease dispersal gradients and consideration of host pattern. The method is extended to provide measures of uncertainty for the estimates of disease at each host location. In addition, improvements have been made to increase computational efficiency by better initialising the disease status of unsampled hosts and speeding up the optimisation process of the model parameters. These improvements facilitate the practical use of the method by providing information on: (a) mechanisms of pathogen dispersal, (b) distance and pattern of disease spread, and (c) prediction of infection probabilities for unsampled hosts. Two data sets of disease observations, Huanglongbing (HLB) of citrus and strawberry powdery mildew, were used to evaluate the performance of the new method for disease mapping. The result showed that our method gave better estimates of precision for unsampled hosts, compared to both the original method and spatial interpolation. This enables decision makers to understand the spatial aspects of disease processes, and thus formulate regulatory actions accordingly to enhance disease control. (C) 2012 Elsevier B.V. All rights reserved. C1 [Luo, W.; Pietravalle, S.] Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England. [Parnell, S.; van den Bosch, F.] Rothamsted Res, Ctr Math & Computat Biol, Harpenden AL5 2JQ, Herts, England. [Luo, W.; Gottwald, T. R.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Trey, M. S.] US Sugar Corp, Clewiston, FL 33440 USA. [Parker, S. R.] Syngenta Crop Protect UK, Cambridge CB21 5XE, England. RP Luo, W (reprint author), Food & Environm Res Agcy, York YO41 1LZ, N Yorkshire, England. EM weiqi.luo@fera.gsi.gov.uk RI parnell, stephen/I-7682-2015 OI parnell, stephen/0000-0002-2625-4557 FU Defra; USDA FX The work was supported by Defra and USDA. The authors would like to thank Dr. Andrew G.S. Cuthbertson (Fera) and two anonymous reviewers for useful comments on the structure of the paper. NR 45 TC 1 Z9 1 U1 0 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1755-4365 J9 EPIDEMICS-NETH JI Epidemics PD JUN PY 2012 VL 4 IS 2 BP 68 EP 77 DI 10.1016/j.epidem.2012.02.001 PG 10 WC Infectious Diseases SC Infectious Diseases GA 966UG UT WOS:000305862300002 PM 22664065 ER PT J AU Boyer, CR Gallagher, TV Gilliam, CH Fain, GB Torbert, HA Sibley, JL AF Boyer, Cheryl R. Gallagher, Thomas V. Gilliam, Charles H. Fain, Glenn B. Torbert, H. Allen Sibley, Jeff L. TI Description of Clean Chip Residual Forest Harvest and Its Availability for Horticultural Uses in the Southeastern United States SO HORTTECHNOLOGY LA English DT Article DE CCR; substrate; alternative media; wood fiber; bark; needles; forestry; nursery; greenhouse ID NURSERY CROP PRODUCTION; PINE TREE SUBSTRATE; FERTILIZER RATE; GREENHOUSE; GROWTH; BARK AB Residual chipping material, also called clean chip residual (CCR), has potential use as a growth substrate in the nursery and greenhouse horticultural industries. A survey was conducted in the southeastern United States among companies conducting harvesting operations on pine (Pinus sp.) plantations for the production of pulpwood in the forest industry. Fourteen operators in four states (Alabama, Mississippi, Georgia, Florida) were visited to evaluate the on-site status of residual material. Sample analysis of CCR revealed that it was composed of approximate to 37.7% wood (range, 14.2% to 50.5%), 36.6% bark (range, 16.1% to 68.5%), 8.8% needles (range, 0.1% to 19.2%), and 16.9% indistinguishable (fine) particles (range, 7.5% to 31%). pH ranged from 4.3 to 5.5 for all locations and electrical conductivity (EC) averaged 0.24 mmho/cm. Most nutrients were in acceptable ranges for plant growth with the exception of three sites above recommended levels for iron and four sites for manganese. Survey participants estimated that approximate to 27.5% of the harvest site biomass was composed of CCR. Some harvesters were able to sell CCR as fuelwood to pulp mills, while others did not recover the residual material and left it on the forest floor (44.3% total site biomass). Operations in this survey included typical pine plantation chipping and grinding operations (harvesters), woodyards (lumber, fuelwood, etc.), and operations processing mixed material (salvage from trees damaged in hurricanes or mixed tree species cleared from a site that was not under management as a plantation). Residual material varied depending on the plantation age, species composition, site quality, and natural actions such as fire. Average tree age was 11.5 years (range, 8 to 15 years), while average tree stand height was 37.0 ft (range, 25 to 50 ft) and average diameter at breast height (DBH) was 5.9 inches (range, 4 to 7 inches). Residual material on site was either sold immediately (28.6%), left on site for 1 to 3 months (28.6%), left on site for up to 2 years (7.1%), or not collected/sold (35.7%). Several loggers were interested in making CCR available to horticultural industries. Adequate resources are available to horticultural industries, rendering the use of CCR in ornamental plant production a viable option. C1 [Boyer, Cheryl R.] Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. [Gallagher, Thomas V.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Gilliam, Charles H.; Fain, Glenn B.; Sibley, Jeff L.] Auburn Univ, Dept Hort, Auburn, AL 36849 USA. [Torbert, H. Allen] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. RP Boyer, CR (reprint author), Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. EM crboyer@ksu.edu NR 25 TC 2 Z9 2 U1 0 U2 3 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2012 VL 22 IS 3 BP 381 EP 387 PG 7 WC Horticulture SC Agriculture GA 967AF UT WOS:000305877800016 ER PT J AU Meer, RV AF Meer, Robert Vander TI Ant Interactions with Soil Organisms and Associated Semiochemicals SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Review DE Formicidae; Microbes; Fungi; Bacteria; Yeast; Parasite; Semiochemical; Defense ID IMPORTED FIRE ANT; SOLENOPSIS-INVICTA BUREN; FUNGUS-GROWING ANTS; LEAF-CUTTING ANTS; METAPLEURAL GLAND SECRETION; VENOM ALKALOIDS; HYMENOPTERA-FORMICIDAE; TRAIL PHEROMONE; THIEF ANTS; ACROMYRMEX-OCTOSPINOSUS AB This review focuses on the semiochemical interactions between ants and their soil environment. Ants occupy virtually every ecological niche and have evolved mechanisms to not just cope with, but also manipulate soil organisms. The metapleural gland, specific to ants was thought to be the major source of semiochemical antimicrobial compounds targeting general or specific deleterious microbes. The extremely diverse variety of semiochemicals and their sources with antimicrobial activity or potential activity is highlighted. The leaf-cutting ants and fire ant provide the most researched species, in part because they cause significant economic damage. The leaf-cutting ant is particularly interesting because researchers have uncovered unexpected interactions between leaf-cutting ant fungal farm, parasitic fungi, bacteria, yeasts, and ant defensive semiochemicals. These complex relationships highlight the multidimensional aspects of ants and the soil environment in which they live. C1 ARS, USDA, Gainesville, FL 32608 USA. RP Meer, RV (reprint author), ARS, USDA, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM bob.vandermeer@ars.usda.gov NR 175 TC 0 Z9 0 U1 4 U2 37 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUN PY 2012 VL 38 IS 6 SI SI BP 728 EP 745 DI 10.1007/s10886-012-0140-8 PG 18 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 958MC UT WOS:000305240900010 ER PT J AU Cotten, TB Kwiatkowski, MA Saenz, D Collyer, M AF Cotten, Taylor B. Kwiatkowski, Matthew A. Saenz, Daniel Collyer, Michael TI Effects of an Invasive Plant, Chinese Tallow (Triadica sebifera), on Development and Survival of Anuran Larvae SO JOURNAL OF HERPETOLOGY LA English DT Article ID INTRODUCED MOSQUITOFISH; POPULATION DECLINES; ADAPTIVE PLASTICITY; AQUATIC COMMUNITIES; AMPHIBIAN DECLINES; NEST PREDATION; LEGGED FROGS; RANA-PIPIENS; METAMORPHOSIS; ATRAZINE AB Amphibians are considered one of the most threatened vertebrate groups. Although numerous studies have addressed the many causes of amphibian population decline, little is known about effects of invasive plants. Chinese tallow (Triadica sebifera) is an exotic deciduous tree that has invaded the southeastern United States. Amphibian larvae in environments invaded by T. sebifera may be impacted negatively as autumn leaf litter decomposes in natal areas. We compared effects of leaf litter decomposition from T. sebifera and two native tree species on survival and development of four species of anuran larvae from eastern Texas. Larvae from Pseudacris fouquettei, Lithobates (Rana) sphenocephahis, Hyla versicolor, and Incilius (Bufo) nebulifer were introduced into mesocosms containing leaf litter from one of the three tree species. Pseudacris fouquettei and L. sphenocephalus, species that breed earlier in the year, had lower survival within the T. sebifera pools. Pseudacris fouquettei were smaller in T. sebifera mesocosms compared with native tree mesocosms, whereas L. sphenocephalus were larger in T. sebifera mesocosms. Hyla versicolor showed significant developmental and morphological differences in T. sebifera mesocosms; however, survival was not significantly different among treatments. Leaf litter treatment did not affect survival or development in I. nebulifer. Our results suggest that breeding season may determine how each species survives and develops in an environment with T. sebifera leaf litter. Triadica sebifera leaf litter breaks down faster than native species; therefore, negative effects may be short lived but pose a greater threat to species that breed soon after leaffall. C1 [Cotten, Taylor B.; Kwiatkowski, Matthew A.; Collyer, Michael] Stephen F Austin State Univ, Dept Biol, Nacogdoches, TX 75962 USA. [Saenz, Daniel] US Forest Serv, So Res Stn, Nacogdoches, TX 75965 USA. RP Kwiatkowski, MA (reprint author), Stephen F Austin State Univ, Dept Biol, POB 13003, Nacogdoches, TX 75962 USA. EM kwiatkowm@sfasu.edu FU U.S. Forest Service Wildlife Habitat and Silviculture Lab in Nacogdoches, Texas; Stephen F. Austin State University FX Collection of specimens was conducted under Texas Parks and Wildlife Department permits SPR 049-0059 and SPR 0708-296. This research was supported by the U.S. Forest Service Wildlife Habitat and Silviculture Lab in Nacogdoches, Texas, and Stephen F. Austin State University. NR 80 TC 15 Z9 15 U1 2 U2 28 PU SOC STUDY AMPHIBIANS REPTILES PI ST LOUIS PA C/O ROBERT D ALDRIDGE, ST LOUIS UNIV, DEPT BIOLOGY, 3507 LACLEDE, ST LOUIS, MO 63103 USA SN 0022-1511 J9 J HERPETOL JI J. Herpetol. PD JUN PY 2012 VL 46 IS 2 BP 186 EP 193 DI 10.1670/10-311 PG 8 WC Zoology SC Zoology GA 969HM UT WOS:000306046300007 ER PT J AU Flerchinger, GN Reba, ML Marks, D AF Flerchinger, Gerald N. Reba, Michele L. Marks, Danny TI Measurement of Surface Energy Fluxes from Two Rangeland Sites and Comparison with a Multilayer Canopy Model SO JOURNAL OF HYDROMETEOROLOGY LA English DT Article ID BOREAL ASPEN FOREST; PRECIPITATION CATCH; COMPLEX TERRAIN; PLANT CANOPIES; SHAW MODEL; WATER; VEGETATION; OVERSTORY; HEAT; BALANCE AB Rangelands are often characterized by a patchy mosaic of vegetation types, making measurement and modeling of surface energy fluxes particularly challenging. The purpose of this study was to evaluate surface energy fluxes measured using three eddy covariance systems above and within two rangeland vegetation sites and use the data to improve simulations of turbulent energy fluxes in a multilayer plant canopy model: the Simultaneous Heat and Water (SHAW) model. Model modifications included adjustment of the wind profile roughness parameters for sparse canopies, extending the currently used K-theory approach to include influence of the roughness sublayer and stability functions within the canopy, and in a separate version of the model, introducing Lagrangian far-field turbulent transfer equations (L theory) in lieu of the K-theory approach. There was relatively little difference in simulated energy fluxes for the aspen canopy using L-theory versus K-theory turbulent transfer equations, but L theory tracked canopy air temperature profiles better during the growing season. Upward sensible heat flux was observed above aspen trees, within the aspen understory, and above sagebrush throughout the active snowmelt season. Model simulations confirmed the observed upward sensible flux during snowmelt was due to solar heating of the aspen limbs and sagebrush. Thus, the eddy covariance (EC) systems were unable to properly quantify fluxes at the snow surface when vegetation was present. Good agreement between measured and modeled energy fluxes suggest that they can be measured and simulated reliably in these complex environments, but care must be used in the interpretation of the results. C1 [Flerchinger, Gerald N.; Marks, Danny] USDA ARS, NW Watershed Res Ctr, Boise, ID 83712 USA. [Reba, Michele L.] USDA ARS, Natl Sedimentat Lab, Jonesboro, AR USA. RP Flerchinger, GN (reprint author), USDA ARS, NW Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. EM gerald.flerchinger@ars.usda.gov NR 49 TC 9 Z9 9 U1 2 U2 13 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1525-755X J9 J HYDROMETEOROL JI J. Hydrometeorol. PD JUN PY 2012 VL 13 IS 3 BP 1038 EP 1051 DI 10.1175/JHM-D-11-093.1 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 965OH UT WOS:000305776600016 ER PT J AU Chambers, DL Ulrey, WA Guthrie, JM Kwok, OCH Cox, JJ Maehr, DS Dubey, JP AF Chambers, D. L. Ulrey, W. A. Guthrie, J. M. Kwok, O. C. H. Cox, J. J. Maehr, D. S. Dubey, J. P. TI Seroprevalence of Toxoplasma gondii From Free-Ranging Black Bears (Ursus americanus) From Florida SO JOURNAL OF PARASITOLOGY LA English DT Article ID WILDLIFE AB Toxoplasma gondil is a significant worldwide parasitic protozoan. In the present study, prevalence of antibodies of T. gondil was examined from 29 free-ranging black bears (Ursus americanus) from south-central Florida where the host species was listed as state threatened during this project. Overall T gondii prevalence was found to be 44.8%, specifically 46.2% in male and 43.8% in female U. americanus, using a modified agglutination test (1:25 titer). Seroprevalence differences between sexes were not significant (P > 0.05). Results of the present study add supportive data to the growing body of evidence suggesting that U. americanus has one of the highest T. gondii seroprevalences among all known intermediate hosts. In addition, our data emphasize the importance of understanding parasitic disease dynamics from a conservation perspective. C1 [Chambers, D. L.] Univ Virginias Coll Wise, Dept Nat Sci, Wise, VA 24293 USA. [Ulrey, W. A.] Archbold Biol Res Stn, Venus, FL 33960 USA. [Ulrey, W. A.; Guthrie, J. M.; Cox, J. J.; Maehr, D. S.] Univ Kentucky, Dept Forestry, Lexington, KY 40546 USA. [Kwok, O. C. H.; Dubey, J. P.] ARS, USDA, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Chambers, DL (reprint author), Univ Virginias Coll Wise, Dept Nat Sci, Wise, VA 24293 USA. EM chambers@uvawise.edu NR 17 TC 1 Z9 1 U1 1 U2 11 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2012 VL 98 IS 3 BP 674 EP 675 DI 10.1645/GE-3046.1 PG 2 WC Parasitology SC Parasitology GA 967RT UT WOS:000305926200036 PM 22150391 ER PT J AU Costa, DGC Marvulo, MFV Silva, JSA Santana, SC Magalhaes, FJR Lima, CDF Ribeiro, VO Alves, LC Mota, RA Dubey, JP Silva, JCR AF Costa, D. G. C. Marvulo, M. F. V. Silva, J. S. A. Santana, S. C. Magalhaes, F. J. R. Lima Filho, C. D. F. Ribeiro, V. O. Alves, L. C. Mota, R. A. Dubey, J. P. Silva, J. C. R. TI Seroprevalence of Toxoplasma gondii in Domestic and Wild Animals From the Fernando de Noronha, Brazil SO JOURNAL OF PARASITOLOGY LA English DT Article ID NEOSPORA-CANINUM ANTIBODIES; NORTHEAST REGION; PREVALENCE; STATE; CHICKENS; PARAIBA; DOGS AB Fernando de Noronha is an archipelago of 21 islands and islets in the Atlantic Ocean, state of Pernambuco, Brazil, which has a varied biodiversity including alien species or sinantropic animals. The objective here was to determine the seroprevalence of Toxoplasma gondii in domestic and wild animals from Fernando de Noronha archipelago, Brazil. Between July 2007 and May 2010, blood samples were collected from 764 animals (533 domestic and 231 wild animals). Sera were tested by the indirect fluorescence antibody test (IFAT) or the modified agglutination test (MAT), or by both. Antibodies to T gondii were found in 80 (80.0%) of 100 chickens (Gallus domesticus), 3 (3.0%) of 100 cattle (Bostaurus), 59 (60.8%) of 97 sheep (Ovis aries), 9 (81.8%) of 11 goats (Capra hircus), 7(43.7%) of 16 horses (Equus caballus), 70 (59.3%) of 118 cats (Fells catus), 36 (39.6%) of 91 dogs (Canis familiaris), 13(38.2%) of 34 black rats (Rattus ram's), and 157 (79.7%) of 197 cattle egrets (Bubulcus ibis). Results indicate endemic infection by this zoonotic parasite among the animal and avian fauna in this archipelago from Brazil. C1 [Costa, D. G. C.; Silva, J. S. A.; Ribeiro, V. O.; Alves, L. C.; Mota, R. A.; Silva, J. C. R.] Univ Fed Rural Pernambuco, Dept Med Vet, BR-52171900 Recife, PE, Brazil. [Marvulo, M. F. V.] Inst Brasileiro Med Conservacao Triade, BR-52061030 Recife, PE, Brazil. [Santana, S. C.; Magalhaes, F. J. R.; Lima Filho, C. D. F.] Distrito Estadual Fernando de Noronha, BR-52120010 Fernando De Noronha, PE, Brazil. [Dubey, J. P.] ARS, USDA, Anim & Nat Resources Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Costa, DGC (reprint author), Univ Fed Rural Pernambuco, Dept Med Vet, BR-52171900 Recife, PE, Brazil. EM jcramos@dmv.ufrpe.br NR 14 TC 25 Z9 27 U1 0 U2 12 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2012 VL 98 IS 3 BP 679 EP 680 DI 10.1645/GE-2910.1 PG 2 WC Parasitology SC Parasitology GA 967RT UT WOS:000305926200038 PM 22150091 ER PT J AU Gunther, NW Paul, M Nunez, A Liu, YH AF Gunther, Nereus W. Paul, Moushumi Nunez, Alberto Liu, Yanhong TI pH Fractionation and identification of proteins: Comparing column chromatofocusing versus liquid isoelectric focusing techniques SO JOURNAL OF SEPARATION SCIENCE LA English DT Article DE Chromatofocusing; Escherichia coli; Isoelectric focusing ID FREE-FLOW; 2-DIMENSIONAL ELECTROPHORESIS; ELECTROKINETIC METHODOLOGIES; SAMPLE PREFRACTIONATION; GEL-ELECTROPHORESIS; MASS-SPECTROMETRY; FUTURE-PROSPECTS; ALPHA-CENTAURY; PROTEOME; CHROMATOGRAPHY AB In proteomic investigations, a number of different separation techniques can be applied to fractionate whole cell proteomes into more manageable fractions for subsequent analysis. In this work, utilizing HPLC and mass spectrometry for protein identification, two different fractionation methods were compared and contrasted to determine the potential of each method for the simple and reproducible fractionation of a bacterial proteome. Column-based chromatofocusing and liquid-based isoelectric focusing both utilized pH gradients to produce similar results in terms of the numbers of proteins successfully identified (402 and 378 proteins) and the consistency of proteins identified from one experiment to the next ( < 10% change). However, there was limited overlap in the protein sets with < 50% of the proteins identified as common between the sets of proteins identified by the different systems. In addition to the numbers of proteins identified and consistency of those identified, the reduced monetary costs of experimentation and increased assay flexibility produced by using isoelectric focusing was considered in order to adopt a system best suited for comparative proteomic projects. C1 [Gunther, Nereus W.; Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Paul, Moushumi] ARS, Dairy & Funct Foods Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Nunez, Alberto] ARS, Core Technol, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Gunther, NW (reprint author), ARS, Mol Characterizat Foodborne Pathogens Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM jack.gunther@ars.usda.gov NR 39 TC 1 Z9 1 U1 1 U2 16 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 1615-9306 J9 J SEP SCI JI J. Sep. Sci. PD JUN PY 2012 VL 35 IS 12 BP 1399 EP 1405 DI 10.1002/jssc.201100865 PG 7 WC Chemistry, Analytical SC Chemistry GA 968UP UT WOS:000306010900001 PM 22740249 ER PT J AU Li, CS Salas, W Zhang, RH Krauter, C Rotz, A Mitloehner, F AF Li, Changsheng Salas, William Zhang, Ruihong Krauter, Charley Rotz, Al Mitloehner, Frank TI Manure-DNDC: a biogeochemical process model for quantifying greenhouse gas and ammonia emissions from livestock manure systems SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Livestock operation systems; Farm scale; Manure life cycle; Greenhouse gases; Ammonia volatilization; DNDC ID NITROUS-OXIDE EMISSIONS; MINERALIZATION POTENTIALS; METHANE PRODUCTION; GASEOUS NITROGEN; CATTLE MANURE; DAIRY MANURE; SOIL; CARBON; DENITRIFICATION; TEMPERATURE AB From the point of view of biogeochemistry, manure is a complex of organic matter containing minor minerals. When manure is excreted by animals, it undergoes a series of reactions such as decomposition, hydrolysis, ammonia volatilization, nitrification, denitrification, fermentation etc., from which carbon dioxide (CO2), nitrous oxide (N2O), methane (CH4) and ammonia (NH3) can be produced. Based on the principles of thermodynamics and reaction kinetics, these reactions are commonly controlled by a group of environmental factors such as temperature, moisture, redox potential, pH, substrate concentration gradient etc. The relations among the environmental factors, the reactions and the gas production have been incorporated in a process-based model, Manure-DNDC, to describe manure organic matter turnover and gas emissions. Using Manure-DNDC, the users can construct a virtual farm by selecting and integrating one or more of the candidate farm facilities (i.e., feedlot, compost, lagoon, anaerobic digester and cropping field) parameterized in the model. Manure-DNDC calculates variations of the environmental factors for each component facility based on its technical specifications, and then utilizes the environmental factors to drive the biogeochemical reactions. To verify the applicability of Manure-DNDC for livestock farms, seven datasets of air emissions measured from farms across the U.S. plus a Scotland pasture were utilized for model tests with encouraging results. A dairy farm in New York was used to assess the impacts of alternative management practices on the gas mitigation. The modeled results showed that a combination of changes in the feed quality, the lagoon coverage and the planted crop type could reduce greenhouse gas emission by 30 % and NH3 by 36 % at the farm scale. C1 [Li, Changsheng] Univ New Hampshire, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. [Salas, William] Appl Geosolut LLC, Durham, NH 03824 USA. [Zhang, Ruihong] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Krauter, Charley] Calif State Univ Fresno, Jordan Coll Agr Sci & Technol, Fresno, CA 93740 USA. [Rotz, Al] USDA ARS, University Pk, PA 16801 USA. [Mitloehner, Frank] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. RP Li, CS (reprint author), Univ New Hampshire, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. EM changsheng.li@unh.edu; wsalas@agsemail.com; rhzhang@ucdavis.cedu; charles@csufresno.edu; al.rotz@ars.usda.gov; fmmitloehner@ucdavis.edu FU National Milk Producer Federation (NMPF); California Energy Commission; USDA National Research Institute (NRI); Dairy Research Institute; USDA NIFA project "Integrated resource management tool to mitigate the carbon footprint of swine produced in the U.S." FX We thank the National Milk Producer Federation (NMPF), California Energy Commission, USDA National Research Institute (NRI) and the Dairy Research Institute for their financial support of the development of the Manure-DNDC model. Thanks are also given to A. Heber and R. Reed for providing field data for model evaluation. The preparation of this manuscript was partially supported by USDA NIFA project "Integrated resource management tool to mitigate the carbon footprint of swine produced in the U.S." NR 120 TC 59 Z9 61 U1 14 U2 149 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD JUN PY 2012 VL 93 IS 2 BP 163 EP 200 DI 10.1007/s10705-012-9507-z PG 38 WC Soil Science SC Agriculture GA 968FF UT WOS:000305962000004 ER PT J AU Wang, Y Brent, CS Fennern, E Amdam, GV AF Wang, Ying Brent, Colin S. Fennern, Erin Amdam, Gro V. TI Gustatory Perception and Fat Body Energy Metabolism Are Jointly Affected by Vitellogenin and Juvenile Hormone in Honey Bees SO PLOS GENETICS LA English DT Article ID DIVISION-OF-LABOR; INSULIN SIGNALING PATHWAY; DEPENDENT PROTEIN-KINASE; APIS-MELLIFERA L.; TIME RT-PCR; SUCROSE RESPONSE THRESHOLDS; RED FLOUR BEETLE; GENE-EXPRESSION; DROSOPHILA-MELANOGASTER; FORAGING BEHAVIOR AB Honey bees (Apis mellifera) provide a system for studying social and food-related behavior. A caste of workers performs age-related tasks: young bees (nurses) usually feed the brood and other adult bees inside the nest, while older bees (foragers) forage outside for pollen, a protein/lipid source, or nectar, a carbohydrate source. The workers' transition from nursing to foraging and their foraging preferences correlate with differences in gustatory perception, metabolic gene expression, and endocrine physiology including the endocrine factors vitellogenin (Vg) and juvenile hormone (JH). However, the understanding of connections among social behavior, energy metabolism, and endocrine factors is incomplete. We used RNA interference (RNAi) to perturb the gene network of Vg and JH to learn more about these connections through effects on gustation, gene transcripts, and physiology. The RNAi perturbation was achieved by single and double knockdown of the genes ultraspiracle (usp) and vg, which encode a putative JH receptor and Vg, respectively. The double knockdown enhanced gustatory perception and elevated hemolymph glucose, trehalose, and JH. We also observed transcriptional responses in insulin like peptide 1 (ilp1), the adipokinetic hormone receptor (AKHR), and cGMP-dependent protein kinase (PKG, or "foraging gene" Amfor). Our study demonstrates that the Vg-JH regulatory module controls changes in carbohydrate metabolism, but not lipid metabolism, when worker bees shift from nursing to foraging. The module is also placed upstream of ilp1, AKHR, and PKG for the first time. As insulin, adipokinetic hormone (AKH), and PKG pathways influence metabolism and gustation in many animals, we propose that honey bees have conserved pathways in carbohydrate metabolism and conserved connections between energy metabolism and gustatory perception. Thus, perhaps the bee can make general contributions to the understanding of food-related behavior and metabolic disorders. C1 [Wang, Ying; Fennern, Erin; Amdam, Gro V.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [Brent, Colin S.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Fennern, Erin] Oregon Hlth & Sci Univ, Sch Med, Portland, OR 97201 USA. [Amdam, Gro V.] Univ Life Sci, Dept Chem Biotechnol & Food Sci, As, Norway. RP Wang, Y (reprint author), Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. EM Ying.Wang.6@asu.edu RI Wang, Ying /H-1172-2012; OI Brent, Colin/0000-0003-2078-1417 FU Research Council of Norway [180504, 185306, 191699]; PEW Charitable Trust; National Institute on Aging (NIA) [P01 AG22500] FX This research was supported by the Research Council of Norway (180504, 185306, 191699), the PEW Charitable Trust, and the National Institute on Aging (NIA P01 AG22500). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 108 TC 29 Z9 31 U1 5 U2 58 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7404 J9 PLOS GENET JI PLoS Genet. PD JUN PY 2012 VL 8 IS 6 AR e1002779 DI 10.1371/journal.pgen.1002779 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 968EW UT WOS:000305961000041 PM 22761585 ER PT J AU Kuzmin, IV Shi, M Orciari, LA Yager, PA Velasco-Villa, A Kuzmina, NA Streicker, DG Bergman, DL Rupprecht, CE AF Kuzmin, Ivan V. Shi, Mang Orciari, Lillian A. Yager, Pamela A. Velasco-Villa, Andres Kuzmina, Natalia A. Streicker, Daniel G. Bergman, David L. Rupprecht, Charles E. TI Molecular Inferences Suggest Multiple Host Shifts of Rabies Viruses from Bats to Mesocarnivores in Arizona during 2001-2009 SO PLOS PATHOGENS LA English DT Article ID MAXIMUM-LIKELIHOOD; EVOLUTIONARY GENETICS; UNITED-STATES; LYSSAVIRUSES; GLYCOPROTEIN; SEQUENCE; IDENTIFICATION; PATHOGENICITY; SURVEILLANCE; DIVERSITY AB In nature, rabies virus (RABV; genus Lyssavirus, family Rhabdoviridae) represents an assemblage of phylogenetic lineages, associated with specific mammalian host species. Although it is generally accepted that RABV evolved originally in bats and further shifted to carnivores, mechanisms of such host shifts are poorly understood, and examples are rarely present in surveillance data. Outbreaks in carnivores caused by a RABV variant, associated with big brown bats, occurred repeatedly during 2001-2009 in the Flagstaff area of Arizona. After each outbreak, extensive control campaigns were undertaken, with no reports of further rabies cases in carnivores for the next several years. However, questions remained whether all outbreaks were caused by a single introduction and further perpetuation of bat RABV in carnivore populations, or each outbreak was caused by an independent introduction of a bat virus. Another question of concern was related to adaptive changes in the RABV genome associated with host shifts. To address these questions, we sequenced and analyzed 66 complete and 20 nearly complete RABV genomes, including those from the Flagstaff area and other similar outbreaks in carnivores, caused by bat RABVs, and representatives of the major RABV lineages circulating in North America and worldwide. Phylogenetic analysis demonstrated that each Flagstaff outbreak was caused by an independent introduction of bat RABV into populations of carnivores. Positive selection analysis confirmed the absence of post-shift changes in RABV genes. In contrast, convergent evolution analysis demonstrated several amino acids in the N, P, G and L proteins, which might be significant for pre-adaptation of bat viruses to cause effective infection in carnivores. The substitution S/T-242 in the viral glycoprotein is of particular merit, as a similar substitution was suggested for pathogenicity of Nishigahara RABV strain. Roles of the amino acid changes, detected in our study, require additional investigations, using reverse genetics and other approaches. C1 [Kuzmin, Ivan V.; Shi, Mang; Orciari, Lillian A.; Yager, Pamela A.; Velasco-Villa, Andres; Kuzmina, Natalia A.; Rupprecht, Charles E.] Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. [Shi, Mang] Penn State Univ, State Coll, PA USA. [Streicker, Daniel G.] Univ Georgia, Athens, GA 30602 USA. [Bergman, David L.] Wildlife Serv, USDA APHIS, Phoenix, AZ USA. RP Kuzmin, IV (reprint author), Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. EM ibk3@cdc.gov RI Shi, Mang/D-5118-2013; Bergman, David/C-6874-2015; OI Bergman, David/0000-0002-6757-643X; Streicker, Daniel/0000-0001-7475-2705 FU US Government FX The study was performed as a part of work of the Rabies Program, Centers for Disease Control and Prevention, and was funded by the US Government. No grants or extramural funding were used. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 40 Z9 40 U1 1 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7374 J9 PLOS PATHOG JI PLoS Pathog. PD JUN PY 2012 VL 8 IS 6 AR e1002786 DI 10.1371/journal.ppat.1002786 PG 11 WC Microbiology; Parasitology; Virology SC Microbiology; Parasitology; Virology GA 968ND UT WOS:000305987800049 PM 22737076 ER PT J AU Zhang, L Seagren, EA Davis, AP Karns, JS AF Zhang, Lan Seagren, Eric A. Davis, Allen P. Karns, Jeffrey S. TI Effects of Temperature on Bacterial Transport and Destruction in Bioretention Media: Field and Laboratory Evaluations SO WATER ENVIRONMENT RESEARCH LA English DT Article DE stormwater; bioretention cells; temperature; fecal coliforms; Escherichia coli; bacterial capture; decay; protozoa; predation ID ESCHERICHIA-COLI O157H7; FECAL-COLIFORM; WATER-QUALITY; SURVIVAL; SOIL; PROTOZOA; MITIGATION; FATE AB Microbial activities are significantly influenced by temperature. This study investigated the effects of temperature on the capture and destruction of bacteria from urban stormwater runoff in bioretention media using 2-year field evaluations coupled with controlled laboratory column studies. Field data from two bioretention cells show that the concentration of indicator bacteria (fecal coliforms and Escherichia coli) was reduced during most storm events, and that the probability of meeting specific water quality criteria in the discharge was increased. Indicator bacteria concentration in the input flow typically increased with higher daily temperature. Although bacterial removal efficiency was independent of temperature in the field and laboratory, column tests showed that bacterial decay coefficients in conventional bioretention media (CBM) increase exponentially with elevated temperature. Increases in levels of protozoa and heterotrophic bacteria associated with increasing temperature appear to contribute to faster die-off of trapped E. coli in CBM via predation and competition. Water Environ. Res., 84, 485 (2012). C1 [Zhang, Lan; Davis, Allen P.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Seagren, Eric A.] Michigan Technol Univ, Dept Civil & Environm Engn, Houghton, MI 49931 USA. [Karns, Jeffrey S.] ARS, USDA, Beltsville, MD USA. EM eseagren@mtu.edu RI DAVIS, ALLEN/F-1066-2017 OI DAVIS, ALLEN/0000-0001-7818-1890 FU National Oceanic and Atmospheric Administration [NA05NOS4191149]; Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET) FX This research was supported by National Oceanic and Atmospheric Administration grant NA05NOS4191149 funded by the Cooperative Institute for Coastal and Estuarine Environmental Technology (CICEET). NR 36 TC 7 Z9 8 U1 1 U2 54 PU WATER ENVIRONMENT FEDERATION PI ALEXANDRIA PA 601 WYTHE ST, ALEXANDRIA, VA 22314-1994 USA SN 1061-4303 J9 WATER ENVIRON RES JI Water Environ. Res. PD JUN PY 2012 VL 84 IS 6 BP 485 EP 496 DI 10.2175/106143012X13280358613589 PG 12 WC Engineering, Environmental; Environmental Sciences; Limnology; Water Resources SC Engineering; Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 969RR UT WOS:000306075100002 PM 22866389 ER PT J AU White, SA Taylor, MD Damrel, DZ AF White, Sarah A. Taylor, Milton D. Damrel, Dixie Z. TI Floral Colonization of a Free-Water Surface Constructed Wetland System in Grady County, Georgia SO CASTANEA LA English DT Article DE Hydrophyte; macrophyte richness; plant nursery; self-design; treatment wetland ID PHOSPHORUS RETENTION; NURSERY RUNOFF; SELF-DESIGN; SUCCESSION; REMOVAL; SCALE AB A comprehensive survey of the vascular flora of a constructed wetland in Grady County, Georgia was conducted at monthly intervals between August 2004 and May 2006. The constructed wetland is used as a part of a nutrient remediation system to improve the sustainability of plant nursery water-handling practices. The survey evaluated vegetation in a 3.1 ha, two-stage constructed wetland consisting of two deep cells (Stage 1; mean depth 0.75 m) totaling 1.8 ha that drain into two shallow cells (Stage 2; mean depth 0.2 m) totaling 1.3 ha. Each cell was band planted in 1997 with seedlings or liners of six wetland plant species. During the 2-yr survey, 141 distinct species were collected from the 3.1 ha constructed wetland; of the taxa collected, there were 101 genera from 51 families. Native taxa made up 76.6% of the species recorded, and 24.1% of the taxa were introduced. Hydrophytic plants accounted for 68.1% of the plants collected and the remainder (31.9%) were classified as nonhydrophytes. Because 57.4% of the taxa surveyed were perennial, it is likely that plant propagules introduced by wildlife (waterfowl, reptiles, and amphibians) were the dominant method of plant introduction rather than primary succession. Vascular plant community diversity increased 24-fold during the 9-yr period after constructed wetland installation. C1 [White, Sarah A.] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29670 USA. [Taylor, Milton D.] Foreign Agr Serv, Civilian Response Corps, USDA, Washington, DC 20250 USA. [Damrel, Dixie Z.] Clemson Univ, Clemson Univ Herbarium, Dept Biol Sci, Clemson, SC 29634 USA. RP White, SA (reprint author), Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29670 USA. EM swhite4@clemson.edu FU Floriculture and Nursery Research Initiative for Environmental and Resource Management Practices and Strategies; NIFA/USDA [SC-1700398]; USDA Agriculture Research Service FX Financial support of this project through the Floriculture and Nursery Research Initiative for Environmental and Resource Management Practices and Strategies, USDA Agriculture Research Service Ft. Pierce, Florida is gratefully acknowledged. Technical Contribution No. 6019 of the Clemson University Experiment Station. This material is based upon work supported by NIFA/USDA, under project number SC-1700398. NR 25 TC 4 Z9 4 U1 1 U2 7 PU SOUTHERN APPALACHIAN BOTANICAL SOC, NEWBERRY COLL PI NEWBERRY PA DEPT BIOLOGY, C/O CHARLES N HORN, SECRETARY-TREASURER, 2100 COLLEGE ST, NEWBERRY, SC 29108 USA SN 0008-7475 J9 CASTANEA JI Castanea PD JUN PY 2012 VL 77 IS 2 BP 159 EP 171 DI 10.2179/11-045 PG 13 WC Plant Sciences SC Plant Sciences GA 962KX UT WOS:000305545100005 ER PT J AU Leakey, ADB Bishop, KA Ainsworth, EA AF Leakey, Andrew D. B. Bishop, Kristen A. Ainsworth, Elizabeth A. TI A multi-biome gap in understanding of crop and ecosystem responses to elevated CO2 SO CURRENT OPINION IN PLANT BIOLOGY LA English DT Review ID CLIMATE-CHANGE; FOREST PRODUCTIVITY; ATMOSPHERIC CO2; CARBON-CYCLE; PLANT CARBON; RISING CO2; STOMATAL CONDUCTANCE; TEMPERATE FOREST; TROPICAL FORESTS; FACE EXPERIMENTS AB A key finding from elevated [CO2] field experiments is that the impact of elevated [CO2] on plant and ecosystem function is highly dependent upon other environmental conditions, namely temperature and the availability of nutrients and soil moisture. In addition, there is significant variation in the response to elevated [CO2] among plant functional types, species and crop varieties. However, experimental data on plant and ecosystem responses to elevated [CO2] are strongly biased to economically and ecologically important systems in the temperate zone. There is a multi-biome gap in experimental data that is most severe in the tropics and subtropics, but also includes high latitudes. Physiological understanding of the environmental conditions and species found at high and low latitudes suggest they may respond differently to elevated [CO2] than well-studied temperate systems. Addressing this knowledge gap should be a high priority as it is vital to understanding 21st century food supply and ecosystem feedbacks on climate change. C1 [Leakey, Andrew D. B.; Bishop, Kristen A.; Ainsworth, Elizabeth A.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Leakey, Andrew D. B.; Bishop, Kristen A.; Ainsworth, Elizabeth A.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Leakey, Andrew D. B.; Ainsworth, Elizabeth A.] ARS, USDA, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Ainsworth, EA (reprint author), Univ Illinois, Dept Plant Biol, 1206 W Gregory Dr, Urbana, IL 61801 USA. EM lisa.ainsworth@ars.usda.gov RI Leakey, Andrew/Q-9889-2016 OI Leakey, Andrew/0000-0001-6251-024X NR 61 TC 19 Z9 21 U1 4 U2 87 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1369-5266 J9 CURR OPIN PLANT BIOL JI Curr. Opin. Plant Biol. PD JUN PY 2012 VL 15 IS 3 BP 228 EP 236 DI 10.1016/j.pbi.2012.01.009 PG 9 WC Plant Sciences SC Plant Sciences GA 964PX UT WOS:000305710300002 PM 22284851 ER PT J AU Zhu, XG Song, QF Ort, DR AF Zhu, Xin-Guang Song, Qingfeng Ort, Donald R. TI Elements of a dynamic systems model of canopy photosynthesis SO CURRENT OPINION IN PLANT BIOLOGY LA English DT Review ID ATMOSPHERIC CO2 CONCENTRATIONS; ELEVATED CO2; CARBON GAIN; PLANT-GROWTH; NITROGEN USE; SOLAR-RADIATION; CROP CANOPIES; LEAF MODELS; LIGHT; C-3 AB Improving photosynthesis throughout the full canopy rather than photosynthesis of only the top leaves of the canopy is central to improving crop yields. Many canopy photosynthesis models have been developed from physiological and ecological perspectives, however most do not consider heterogeneities of microclimatic factors inside a canopy, canopy dynamics and associated energetics, or competition among different plants, and most models lack a direct linkage to molecular processes. Here we described the rationale, elements, and approaches necessary to build a dynamic systems model of canopy photosynthesis. A systems model should integrate metabolic processes including photosynthesis, respiration, nitrogen metabolism, resource remobilization and photosynthate partitioning with canopy level light, CO2, water vapor distributions and heat exchange processes. In so doing a systems-based canopy photosynthesis model will enable studies of molecular ecology and dramatically improve our insight into engineering crops for improved canopy photosynthetic CO2 uptake, resource use efficiencies and yields. C1 [Zhu, Xin-Guang; Song, Qingfeng] Chinese Acad Sci, State Key Lab Hybrid Rice Res, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China. [Zhu, Xin-Guang; Song, Qingfeng] Chinese Acad Sci, CAS Key Lab Computat Biol, CAS MPG Chinese Acad Sci German Max Planck Soc, Partner Inst Computat Biol,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China. [Ort, Donald R.] Univ Illinois, Inst Genom Biol, Global Change & Photosynth Res Unit, USDA ARS,Dept Plant Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Inst Genom Biol, Global Change & Photosynth Res Unit, USDA ARS,Dept Crop Sci, Urbana, IL 61801 USA. RP Zhu, XG (reprint author), Chinese Acad Sci, State Key Lab Hybrid Rice Res, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci, Yueyang Rd 320, Shanghai 200031, Peoples R China. EM zhuxinguang@picb.ac.cn FU National Science Foundation of China [30970213]; Ministry of Science and Technology of China [2011DFA31070]; Bill & Melinda Gates Foundation [OPP1014417]; Young Talent Frontier Program of Shanghai Institutes for Biology Sciences/Chinese Academy of Sciences [09Y1C11501]; CAS; United States Department of Agriculture, Agricultural Research Service FX Funding for authors' research is from National Science Foundation of China (Grant No. 30970213), Ministry of Science and Technology of China (Grant No. 2011DFA31070), the Bill & Melinda Gates Foundation (Grant No. OPP1014417), and the Young Talent Frontier Program of Shanghai Institutes for Biology Sciences/Chinese Academy of Sciences (Grant No. 09Y1C11501), and a CAS visiting professorship grant to DRO and the United States Department of Agriculture, Agricultural Research Service. NR 80 TC 25 Z9 25 U1 4 U2 60 PU CURRENT BIOLOGY LTD PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 1369-5266 J9 CURR OPIN PLANT BIOL JI Curr. Opin. Plant Biol. PD JUN PY 2012 VL 15 IS 3 BP 237 EP 244 DI 10.1016/j.pbi.2012.01.010 PG 8 WC Plant Sciences SC Plant Sciences GA 964PX UT WOS:000305710300003 PM 22325454 ER PT J AU Green, BT Lee, ST Panter, KE Brown, DR AF Green, Benedict T. Lee, Stephen T. Panter, Kip E. Brown, David R. TI Piperidine alkaloids: Human and food animal teratogens SO FOOD AND CHEMICAL TOXICOLOGY LA English DT Review DE Anabaseine; Anabasine; Coniine; gamma-Coniceine; N-acetylhystrine; Nicotinic acetylcholine receptors ID NICOTINE REPLACEMENT THERAPY; HEMLOCK CONIUM-MACULATUM; MINOR TOBACCO ALKALOIDS; GAS-CHROMATOGRAPHY/MASS SPECTROMETRY; TANDEM MASS-SPECTROMETRY; WILD TREE TOBACCO; POISON-HEMLOCK; ACETYLCHOLINE-RECEPTORS; CONGENITAL ARTHROGRYPOSIS; BIRTH-DEFECTS AB Piperidine alkaloids are acutely toxic to adult livestock species and produce musculoskeletal deformities in neonatal animals. These teratogenic effects include multiple congenital contracture (MCC) deformities and cleft palate in cattle, pigs, sheep, and goats. Poisonous plants containing teratogenic piperidine alkaloids include poison hemlock (Conium maculatum), lupine (Lupinus spp.), and tobacco (Nicotiana tabacum) [including wild tree tobacco (Nicotiana glauca)]. There is abundant epidemiological evidence in humans that link maternal tobacco use with a high incidence of oral clefting in newborns; this association may be partly attributable to the presence of piperidine alkaloids in tobacco products. In this review, we summarize the evidence for piperidine alkaloids that act as teratogens in livestock, piperidine alkaloid structure-activity relationships and their potential implications for human health. Published by Elsevier Ltd. C1 [Green, Benedict T.; Lee, Stephen T.; Panter, Kip E.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Brown, David R.] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. RP Green, BT (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM Ben.Green@ars.usda.gov FU USDA/ARS FX The authors thank Terrie Wierenga for expert assistance with the figures and Isabelle McCollum and Amber Whittaker for assistance with the manuscript. This work was supported by USDA/ARS. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 103 TC 14 Z9 14 U1 3 U2 51 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0278-6915 J9 FOOD CHEM TOXICOL JI Food Chem. Toxicol. PD JUN PY 2012 VL 50 IS 6 BP 2049 EP 2055 DI 10.1016/j.fct.2012.03.049 PG 7 WC Food Science & Technology; Toxicology SC Food Science & Technology; Toxicology GA 964TN UT WOS:000305719700033 PM 22449544 ER PT J AU Van Hekken, DL AF Van Hekken, Diane L. TI Quality Aspects of RAW MILK CHEESES SO FOOD TECHNOLOGY LA English DT Article ID PASTEURIZED MILK; CHEDDAR CHEESE; TEMPERATURE; FLAVOR C1 ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Van Hekken, DL (reprint author), ARS, Dairy & Funct Foods Res Unit, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM diane.vanhekken@ars.usda.gov NR 34 TC 2 Z9 2 U1 0 U2 6 PU INST FOOD TECHNOLOGISTS PI CHICAGO PA 525 WEST VAN BUREN, STE 1000, CHICAGO, IL 60607-3814 USA SN 0015-6639 J9 FOOD TECHNOL-CHICAGO JI Food Technol. PD JUN PY 2012 VL 66 IS 6 BP 66 EP + PG 8 WC Food Science & Technology SC Food Science & Technology GA 960HN UT WOS:000305378700015 ER PT J AU Kwon, SJ Brown, AF Hu, J McGee, R Watt, C Kisha, T Timmerman-Vaughan, G Grusak, M McPhee, KE Coyne, CJ AF Kwon, Soon-Jae Brown, Allan F. Hu, Jinguo McGee, Rebecca Watt, Chasity Kisha, Ted Timmerman-Vaughan, Gail Grusak, Michael McPhee, Kevin E. Coyne, Clarice J. TI Genetic diversity, population structure and genome-wide marker-trait association analysis emphasizing seed nutrients of the USDA pea (Pisum sativum L.) core collection SO GENES & GENOMICS LA English DT Article DE Genetic diversity; Legume; Population structure; Association study; Core collection ID MULTILOCUS GENOTYPE DATA; LINKAGE DISEQUILIBRIUM; ROOT-ROT; GERMPLASM COLLECTIONS; ARABIDOPSIS-THALIANA; PARTIAL RESISTANCE; MOLECULAR MARKERS; CROP IMPROVEMENT; FLOWERING TIME; POLYMORPHISM AB Genetic diversity, population structure and genome-wide marker-trait association analysis was conducted for the USDA pea (Pisum sativum L.) core collection. The core collection contained 285 accessions with diverse phenotypes and geographic origins. The 137 DNA markers included 102 polymorphic fragments amplified by 15 microsatellite primer pairs, 36 RAPD loci and one SCAR (sequence characterized amplified region) marker. The 49 phenotypic traits fall into the categories of seed macro- and micro-nutrients, disease resistance, agronomic traits and seed characteristics. Genetic diversity, population structure and marker-trait association were analyzed with the software packages PowerMarker, STUCTURE and TASSEL, respectively. A great amount of variation was revealed by the DNA markers at the molecular level. Identified were three sub-populations that constituted 56.1%, 13.0% and 30.9%, respectively, of the USDA Pisum core collection. The first sub-population is comprised of all cultivated pea varieties and landraces; the second of wild P. sativum ssp. elatius and abyssinicum and the accessions from the Asian highland (Afghanistan, India, Pakistan, China and Nepal); while the third is an admixture containing alleles from the first and second sub-populations. This structure was achieved using a stringent cutoff point of 15% admixture (q-value 85%) of the collection. Significant marker-trait associations were identified among certain markers with eight mineral nutrient concentrations in seed and other important phenotypic traits. Fifteen pairs of associations were at the significant levels of P a parts per thousand currency sign 0.01 when tested using the three statistical models. These markers will be useful in marker-assisted selection to breed pea cultivars with desirable agronomic traits and end-user qualities. C1 [Kwon, Soon-Jae; Hu, Jinguo; Kisha, Ted; Coyne, Clarice J.] Washington State Univ, USDA ARS Western Reg Plant Intro Stn, Pullman, WA 99164 USA. [Brown, Allan F.] N Carolina State Univ, Plants Human Hlth Inst, Kannapolis, NC 28081 USA. [McGee, Rebecca] Washington State Univ, USDA ARS, Grain Legume Genet Physiol Res, Pullman, WA 99164 USA. [Watt, Chasity] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Timmerman-Vaughan, Gail] New Zealand Inst Plant & Food Res, Christchurch 8140, New Zealand. [Grusak, Michael] ARS, USDA, Children Nutr Res Ctr, Houston, TX 77030 USA. [McPhee, Kevin E.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Coyne, CJ (reprint author), Washington State Univ, USDA ARS Western Reg Plant Intro Stn, Mail Stop 646402, Pullman, WA 99164 USA. EM clarice.coyne@ars.usda.gov RI Timmerman-Vaughan, Gail/J-7574-2013; OI Timmerman-Vaughan, Gail/0000-0001-5538-6383 FU USDA-ARS; USDA-ARS Pacific West Summer Internship; USDA Foreign Agricultural Service; CRIS [5348-21000-017-00D, 5348-21000-024-00D] FX The authors wish to acknowledge their funding sources: USDA-ARS post-doctoral fellowships (S-JK and AFB); USDA-ARS Pacific West Summer Internship (CW); USDA Foreign Agricultural Service Scientific Cooperation Exchange Program (GTV, CJC) and funding from CRIS Projects #5348-21000-017-00D (JH, SJK, TK, CJC) and #5348-21000-024-00D (RJM). For technical assistance, the authors wish to thank Landon Charlo and Michael Cashman for their morphological data collection, as well as Leon Razai and Lisa Taylor for their laboratory assistance. NR 88 TC 17 Z9 18 U1 1 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1976-9571 EI 2092-9293 J9 GENES GENOM JI Genes Genom. PD JUN PY 2012 VL 34 IS 3 BP 305 EP 320 DI 10.1007/s13258-011-0213-z PG 16 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Genetics & Heredity GA 964LA UT WOS:000305695300009 ER PT J AU Basappa, J Graham, CE Turcan, S Vetter, DE AF Basappa, Johnvesly Graham, Christine E. Turcan, Sevin Vetter, Douglas E. TI The cochlea as an independent neuroendocrine organ: Expression and possible roles of a local hypothalamic-pituitary-adrenal axis-equivalent signaling system SO HEARING RESEARCH LA English DT Review ID CORTICOTROPIN-RELEASING-FACTOR; OUTER HAIR-CELLS; SURFACE AMPA RECEPTORS; AUDITORY-NERVE FIBERS; ANXIETY-LIKE BEHAVIOR; INDUCED HEARING-LOSS; INNER-EAR; ACOUSTIC INJURY; GLUCOCORTICOID-RECEPTORS; GUINEA-PIG AB A key property possessed by the mammalian cochlea is its ability to dynamically alter its own sensitivity. Because hair cells and ganglion cells are prone to damage following exposure to loud sound, extant mechanisms limiting cochlear damage include modulation involving both the mechanical (via outer hair cell motility) and neural signaling (via inner hair cell-ganglion cell synapses) steps of peripheral auditory processing. Feedback systems such as that embodied by the olivocochlear system can alter sensitivity, but respond only after stimulus encoding, allowing potentially damaging sounds to impact the inner ear before sensitivity is adjusted. Less well characterized are potential cellular signaling systems involved in protection against metabolic stress and resultant damage. Although pharmacological manipulation of the olivocochlear system may hold some promise for attenuating cochlear damage, targeting this system may still allow damage to occur that does not depend on a fully functional feedback loop for its mitigation. Thus, understanding endogenous cell signaling systems involved in cochlear protection may lead to new strategies and therapies for prevention of cochlear damage and consequent hearing loss. We have recently discovered a novel cochlear signaling system that is molecularly equivalent to the classic hypothalamic-pituitary-adrenal (HPA) axis. This cochlear HPA-equivalent system functions to balance auditory sensitivity and susceptibility to noise-induced hearing loss, and also protects against cellular metabolic insults resulting from exposures to ototoxic drugs. This system may represent a local cellular response system designed to mitigate damage arising from various types of insult. (C) 2012 Elsevier B.V. All rights reserved. C1 [Vetter, Douglas E.] Univ Mississippi, Med Ctr, Dept Neurobiol & Anat Sci, Jackson, MS 39216 USA. [Basappa, Johnvesly] Univ Michigan, Sch Med, Dept Neurol, Ann Arbor, MI USA. [Graham, Christine E.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Turcan, Sevin] Mem Sloan Kettering Canc Ctr, Human Oncol & Pathogenesis Program, New York, NY 10021 USA. RP Vetter, DE (reprint author), Univ Mississippi, Med Ctr, Dept Neurobiol & Anat Sci, 2500 N State St, Jackson, MS 39216 USA. EM dvetter@urnc.edu OI Turcan, Sevin/0000-0002-0885-5607 FU NIH [R01DC006258]; Richard and Susan Smith Family Foundation; Univ. Mississippi Medical Center FX We wish to acknowledge Dr. M.C. Liberman for his generous support in granting access to and use of his auditory physiology setup for all of the studies described in this review, Dr. Paul Sawchenko for providing CRFR1-GFP transgenic mice, Dr. Carlos Arias for skillful assistance with the CRFR1-GFP mice, and Dr. Kuo-Fen Lee and the late Dr. Wylie Vale for providing initial CRFR1 and CRFR2 null lines used to generate the mice used in these studies. This work was funded by NIH R01DC006258 (to DEV), The Richard and Susan Smith Family Foundation (to DEV), and research funding from Univ. Mississippi Medical Center (to DEV). The reader is advised that some figures and portions of text have appeared in previous publications of the authors (Basappa et al., 2010; Graham et al., 2010, 2011; Graham and Vetter, 2011), and are reproduced in this invited review with permission. NR 96 TC 3 Z9 3 U1 0 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-5955 J9 HEARING RES JI Hear. Res. PD JUN PY 2012 VL 288 IS 1-2 SI SI BP 3 EP 18 DI 10.1016/j.heares.2012.03.007 PG 16 WC Audiology & Speech-Language Pathology; Neurosciences; Otorhinolaryngology SC Audiology & Speech-Language Pathology; Neurosciences & Neurology; Otorhinolaryngology GA 966WW UT WOS:000305869100002 PM 22484018 ER PT J AU McKenzie, CL Bethke, JA Byrne, FJ Chamberlin, JR Dennehy, TJ Dickey, AM Gilrein, D Hall, PM Ludwig, S Oetting, RD Osborne, LS Schmale, L Shatters, RG AF McKenzie, Cindy L. Bethke, James A. Byrne, Frank J. Chamberlin, Joseph R. Dennehy, Timothy J. Dickey, Aaron M. Gilrein, Dan Hall, Paula M. Ludwig, Scott Oetting, Ronald D. Osborne, Lance S. Schmale, Lin Shatters, Robert G., Jr. TI Distribution of Bemisia tabaci (Hemiptera: Aleyrodidae) Biotypes in North America After the Q Invasion SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bemisia argentifolii; microsatellite marker; mitochondrial cytochrome oxidase I subunit; New World biotype ID EXOTIC Q-BIOTYPE; GENETIC-STRUCTURE; GENNADIUS HEMIPTERA; PYRIPROXYFEN RESISTANCE; MORPHOLOGICAL VARIATION; NEW-ZEALAND; 1ST REPORT; B-BIOTYPE; RAPD-PCR; POPULATIONS AB After the 2004 discovery of the Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Q biotype in the United States, there was a vital need to determine the geographical and host distribution as well as its interaction with the resident B biotype because of its innate ability to rapidly develop high-level insecticide resistance that persists in the absence of exposure. As part of a coordinated country-wide effort, an extensive survey of B. tabaci biotypes was conducted in North America, with the cooperation of growers, industry, local, state, and federal agencies, to monitor the introduction and distribution of the Q biotype. The biotype status of submitted B. tabaci samples was determined either by polymerase chain reaction amplification and sequencing of a mitochondrial cytochrome oxidase I small subunit gene fragment and characterization of two biotype discriminating nuclear microsatellite markers or esterase zymogram analysis. Two hundred and eighty collections were sampled from the United States, Bermuda, Canada, and Mexico during January 2005 through December 2011. Host plants were split between ornamental plant and culinary herb (67%) and vegetable and field crop (33%) commodities. The New World biotype was detected on field-grown tomatoes (Solanum lycopersicum L.) in Mexico (two) and in commercial greenhouses in Texas (three) and represented 100% of these five collections. To our knowledge, the latter identification represents the first report of the New World biotype in the United States since its rapid displacement in the late 1980s after the introduction of biotype B. Seventy-one percent of all collections contained at least one biotype B individual, and 53% of all collections contained only biotype B whiteflies. Biotype Q was detected in 23 states in the United States, Canada (British Columbia and Ontario territories), Bermuda, and Mexico. Forty-five percent of all collections were found to contain biotype Q in samples from ornamentals, herbs and a single collection from tomato transplants located in protected commercial horticultural greenhouses, but there were no Q detections in outdoor agriculture (vegetable or field crops). Ten of the 15 collections (67%) from Canada and a single collection from Bermuda contained biotype Q, representing the first reports of biotype Q for both countries. Three distinct mitochondrial haplotypes of B. tabaci biotype Q whiteflies were detected in North America. Our data are consistent with the inference of independent invasions from at least three different locations. Of the 4,641 individuals analyzed from 517 collections that include data from our previous work, only 16 individuals contained genetic or zymogram evidence of possible hybridization of the Q and B biotypes, and there was no evidence that rare hybrid B-Q marker co-occurrences persisted in any populations. C1 [McKenzie, Cindy L.; Dickey, Aaron M.; Shatters, Robert G., Jr.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Bethke, James A.] Univ Calif Cooperat Extens, San Diego, CA 92078 USA. [Byrne, Frank J.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Chamberlin, Joseph R.] Valent USA Corp, Snellville, GA 30078 USA. [Dennehy, Timothy J.] Bayer Crop Sci LP, Res Triangle Pk, NC 27709 USA. [Gilrein, Dan] Cornell Univ Cooperat Extens Suffolk Cty, Long Isl Hort Res & Extens Ctr, Riverhead, NY 11901 USA. [Hall, Paula M.; Osborne, Lance S.] Univ Florida, IFAS, Mid Florida Res & Extens Ctr, Apopka, FL 32703 USA. [Ludwig, Scott] Nichino Amer Inc, Arp, TX 75750 USA. [Oetting, Ronald D.] Univ Georgia, Coll Agr & Environm Sci, Griffin, GA 30223 USA. [Schmale, Lin] Soc Amer Florists, Alexandria, VA 22314 USA. RP McKenzie, CL (reprint author), USDA ARS, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM cindy.mckenzie@ars.usda.gov FU Floriculture and Nursery Research Initiative FX We especially thank all the anonymous growers who sent in whitefly samples to be analyzed. Without their support and dedication to the ornamental industry, determining the extent of the distribution of biotype Q in North America would not have been possible. We also thank APHIS; Glades Crop Care; Florida Department of Agriculture and Consumer Services Division of Plant Industry; Canada Plant Diagnostic Laboratories in British Columbia and Ontario, Ministry of Agriculture and Lands; and Bermuda Department of Environmental Protection and their staff for sending whitefly submissions for identification. We thank John Prokop, Laurie Keidong, Tina Grigsby, Michael Cartwright, Arron Cole, and Ankush Zutshi (USDA ARS, U.S. Horticultural Research Laboratory, Fort Pierce, FL) for dedicated technical laboratory assistance. This research was partially funded by the Floriculture and Nursery Research Initiative. NR 77 TC 29 Z9 31 U1 2 U2 29 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 753 EP 766 DI 10.1603/EC11337 PG 14 WC Entomology SC Entomology GA 961NY UT WOS:000305473500002 PM 22812110 ER PT J AU Srinivasan, R Hall, DG Cervantes, FA Alvarez, JM Whitworth, JL AF Srinivasan, Rajagopalbabu Hall, Darren G. Cervantes, Felix A. Alvarez, Juan M. Whitworth, Jonathan L. TI Strain Specificity and Simultaneous Transmission of Closely Related Strains of a Potyvirus by Myzus persicae SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE aphid; Potyvirus-transmission; specificity; interference ID POTATO-VIRUS-Y; TOBACCO ETCH VIRUS; APHID TRANSMISSION; COAT PROTEIN; CAPSID PROTEIN; UNITED-STATES; PVYN-O; INTERFERENCE; EFFICIENCY; VECTORS AB Potato virus Y (PVY), a Potyvirus, is transmitted by aphids in a nonpersistent manner. PVY severely affects potato production worldwide. Single and mixed infections of PVY strains, namely PVYO, PVYNTN, and PVYN:O are a common occurrence in potato systems. However, information available on the ability of aphids to simultaneously transmit multiple PVY strains, specificity associated with simultaneous transmission, and factors affecting specificity are limited. Aphid-mediated transmission experiments were conducted to test the ability of individual aphids to transmit multiple strains using a PVY indicator host. Preliminary results revealed that aphids can transmit at least two viral strains simultaneously. Subsequently, aphid-mediated transmission of three dual-strain combinations was tested using potato plants. Individual aphids transmitted two viral strains simultaneously for all three dual-strain combinations. In all aphid-mediated dual-strain infections involving PVYNTN, the rate of PVYNTN infection was greater than the infection rates of the second strain and dual-strain combinations, indicating specificity associated with transmission of PVY strains. Results of aphid-mediated transmission experiments were compared with results obtained through mechanical transmission. In general, PVY infection rates from aphid-mediated transmission were lower than the rates obtained through mechanical transmission. Unlike aphid-mediated transmission, component strains in dual-strain inoculations were not eliminated during mechanical transmission. These results suggest that there may also be interference associated with aphid-mediated transmission of closely related PVY strains. Perhaps, the observed specificity and/or interference may explain the increase in the incidence of PVYNTN and other necrotic strains in recent years. C1 [Hall, Darren G.] Univ Idaho, Aberdeen R&E Ctr, USDA ARS, Aberdeen, ID 83210 USA. RP Srinivasan, R (reprint author), Univ Georgia, Dept Entomol, 2360 Rainwater Rd, Tifton, GA 31793 USA. EM babusri@uga.edu NR 34 TC 6 Z9 6 U1 4 U2 28 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 783 EP 791 DI 10.1603/EC11310 PG 9 WC Entomology SC Entomology GA 961NY UT WOS:000305473500005 PM 22812113 ER PT J AU Liu, YB AF Liu, Yong-Biao TI Oxygenated Phosphine Fumigation for Control of Nasonovia ribisnigri (Homoptera: Aphididae) on Harvested Lettuce SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE phosphine fumigation; Nasonovia ribisnigri; postharvest pest control; lettuce; phytosanitary ID POSTHARVEST CONTROL; TOXICITY; INSECTS; LIMITS AB Low temperature regular phosphine fumigations under the normal oxygen level and oxygenated phosphine fumigations under superatmospheric oxygen levels were compared for efficacy against the aphid, Nasonovia ribisnigri (Mosley), and effects on postharvest quality of romaine and head lettuce. Low temperature regular phosphine fumigation was effective against the aphid. However, a 3 d treatment with high phosphine concentrations of >= 2,000 ppm was needed for complete control of the aphid. Oxygen greatly increased phosphine toxicity and significantly reduced both treatment time and phosphine concentration for control of N. ribisnigri. At 1,000 ppm phosphine, 72 h regular fumigations at 6 degrees C did not achieve 100% mortality of the aphid. The 1,000 ppm phosphine fumigation under 60% O-2 killed all aphids in 30 h. Both a 72 h regular fumigation with 2,200 ppm phosphine and a 48 h oxygenated fumigation with 1,000 ppm phosphine under 60% O-2 were tested on romaine and head lettuce at 3 degrees C. Both treatments achieved complete control of N. ribisnigri. However, the 72 h regular fumigation resulted in significantly higher percentages of lettuce with injuries and significantly lower lettuce internal quality scores than the 48 h oxygenated phosphine fumigation. Although the oxygenated phosphine fumigation also caused injuries to some treated lettuce, lettuce quality remained very good and the treatment is not expected to have a significant impact on marketability of the lettuce. This study demonstrated that oxygenated phosphine fumigation was more effective and less phytotoxic for controlling N. ribisnigri on harvested lettuce than regular phosphine fumigation and is promising for practical use. C1 USDA ARS, Salinas, CA 93905 USA. RP Liu, YB (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM Yongbiao.liu@ars.usda.gov FU U.S. Department of Agriculture, Foreign Agricultural Service FX I would like to thank T. Masuda and J. Wasson for technical assistance and Tanimura & Antle Co. (Salinas, CA) for providing lettuce used in the study. I would also like to thanks S. S. Walse and J. Tebbets for technical assistance in GC. This study was supported in part by funding from U.S. Department of Agriculture, Foreign Agricultural Service. NR 19 TC 8 Z9 10 U1 0 U2 4 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 810 EP 816 DI 10.1603/EC11328 PG 7 WC Entomology SC Entomology GA 961NY UT WOS:000305473500008 PM 22812116 ER PT J AU Calcaterra, LA Coulin, C Briano, JA Follett, PA AF Calcaterra, Luis A. Coulin, Carolina Briano, Juan A. Follett, Peter A. TI Acute Exposure to Low-Dose Radiation Disrupts Reproduction and Shortens Survival of Wasmannia auropunctata (Hymenoptera: Formicidae) Queens SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE little fire ant; quarantine pest; irradiation; phytosanitary treatment; invasive ant ID IRRADIATION AB Irradiation is a postharvest quarantine treatment option to control ants and other hitchhiker pests on fresh horticultural products exported from Hawaii. The radiotolerance of the invasive little fire ant, Wasmannia auropunctata (Roger) (Hymenoptera: Formicidae: Myrmicinae), was studied to determine a dose sufficient for its control. Queens from each of five laboratory microcolonies started from five geographic locations in Argentina were irradiated at 20, 50, 70, or 100 Gy or left untreated as controls and then followed for 13 wk to observe colony growth. In general, queen survivorship, and the number of eggs, larvae, and pupae observed in the microcolonies decreased with increasing irradiation dose. In the 50-, 70-, and 100-Gy treatments, the number of eggs observed was reduced by 68, 66, and 76%, respectively, compared with untreated control microcolonies. The number of larvae in the 50-, 70-, and, 100-Gy treatments was reduced by 99.6%, and only one pupa was observed in the 50-Gy treatment and none in the 70-and 100-Gy treatments during the 13-wk experiment. Queens in the 100-Gy treatment had significantly reduced longevity compared with queens in the other treatments. Radiation doses >= 70 Gy stopped reproduction in W. auropunctata queens and should be sufficient as a phytosanitary treatment. Information from additional invasive ants in Myrmicinae and other subfamilies is needed before recommending a generic irradiation treatment for ants. C1 [Calcaterra, Luis A.; Coulin, Carolina; Briano, Juan A.] USDA ARS, US S Amer Biol Control Lab, Buenos Aires, DF, Argentina. [Follett, Peter A.] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Follett, PA (reprint author), USDA ARS, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM peter.follett@ars.usda.gov NR 20 TC 4 Z9 4 U1 2 U2 12 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 817 EP 822 DI 10.1603/EC11374 PG 6 WC Entomology SC Entomology GA 961NY UT WOS:000305473500009 PM 22812117 ER PT J AU Graham, EE Poland, TM McCullough, DG Millar, JG AF Graham, Elizabeth E. Poland, Therese M. McCullough, Deborah G. Millar, Jocelyn G. TI A Comparison of Trap Type and Height for Capturing Cerambycid Beetles (Coleoptera) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Cerambycidae; wood-borer detection; trapping; monitoring; flight-intercept trap ID TETROPIUM-FUSCUM F; UNITED-STATES; ANOPLOPHORA-GLABRIPENNIS; AGGREGATION PHEROMONES; INSECT INVASIONS; CASTANEUM L.; RELEASE RATE; PANEL TRAPS; ECOLOGY; PLANT AB Wood-boring beetles in the family Cerambycidae (Coleoptera) play important roles in many forest ecosystems. However, increasing numbers of invasive cerambycid species are transported to new countries by global commerce and threaten forest health in the United States and worldwide. Our goal was to identify effective detection tools for a broad array of cerambycid species by testing some known cerambycid attractants and a pheromone in different trap designs placed across a range of habitats. We compared numbers and species richness of cerambycid beetles captured with cross-vane panel traps and 12-unit Lindgren multiple-funnel traps, placed either at ground level (1.5 m high) or canopy level (approximate to 3 10 m high), at eight sites classified as either residential, industrial, deciduous forest, or conifer forest. We captured 3,723 beetles representing 72 cerambycid species from 10 June to 15 July 2010. Species richness was highest for the subfamilies Cerambycinae and Lamiinae, which accounted for 33 and 46% of all species captured, respectively. Overall, the cross-vane panel traps captured approximate to 1.5 times more beetles than funnel traps. Twenty-one species were captured exclusively in traps at one height, either in the canopy or at ground level. More species were captured in hardwood sites (59 species) where a greater diversity of host material was available than in conifer (34 species), residential (41 species), or industrial (49) sites. Low numbers of beetles (n < 5) were recorded for 28 of the beetle species. The number of species captured per week ranged from 49 species on 21 June to 37 species on 12 July. Cross-vane panel traps installed across a vertical gradient should maximize the number of cerambycid species captured. C1 [Graham, Elizabeth E.; McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48823 USA. [Poland, Therese M.] US Forest Serv, USDA, E Lansing, MI 48823 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48823 USA. [Millar, Jocelyn G.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Graham, EE (reprint author), Michigan State Univ, Dept Entomol, 1407 S Harrison Rd,Rm 220, E Lansing, MI 48823 USA. EM graha139@msu.edu FU USDA Forest Service, Northeastern Area, Special Technology Development Program FX We thank Tina Ciaramitaro and Tom Baweja for their assistance in the field, Greg Kowalewski for access to the Kellogg Experimental Forest, Lawrence Hanks for his input on the study design, and Matthew Ginzel and Daniel Miller for their critical review of an earlier version of the manuscript. This study was supported by the USDA Forest Service, Northeastern Area, Special Technology Development Program. NR 40 TC 21 Z9 23 U1 2 U2 41 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 837 EP 846 DI 10.1603/EC12053 PG 10 WC Entomology SC Entomology GA 961NY UT WOS:000305473500011 PM 22812119 ER PT J AU Cooper, WR Spurgeon, DW AF Cooper, W. Rodney Spurgeon, Dale W. TI Temperature Dependent Development of Lygus hesperus (Hemiptera: Miridae) Nymphs SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE biophysical model; temperature-dependent development; western tarnished plant bug; degree-day model ID HEAT ACCUMULATION; COTTON; WEEDS AB Lygus hesperus Knight (Hemiptera: Miridae) is a key pest of fruit and vegetable crops, forages, and cotton (Gossypium spp.) in the western United States. Accurate models describing relationships between temperature and L. hesperus development are critical to the study of seasonal L. hesperus population dynamics. Development of L. hesperus nymphs was assessed at nine constant temperatures from 10 to 37.8 degrees C. The relationships between temperature and development for each L. hesperus instar, and for the entire nymphal stage, were best described by six-parameter biophysical models indicating both low-and high-temperature inhibition of development. Development rates asymptotically approached zero with decreasing temperature in the lower thermal range, and decreased with increasing temperatures above 32.2 degrees C. Nymphs did not survive from egg hatch to adulthood at either 10 or 37.8 degrees C, and nymph mortality was >90% at both 12.8 and 35.0 degrees C. The fifth instar exhibited the longest stadium, whereas the shortest stadia were associated with the second and third instars. Development rates of males and females did not differ, and the ratio of males to females was not different from 1:1 at any temperature. Our temperature-dependent development rate models for L. hesperus nymphs will facilitate control of insect physiological age in controlled laboratory experiments, and should be useful in planning and interpreting field studies on L. hesperus population dynamics. C1 [Cooper, W. Rodney; Spurgeon, Dale W.] USDA ARS, Shafter, CA 93263 USA. RP Cooper, WR (reprint author), USDA ARS, 17053 N Shafter Ave, Shafter, CA 93263 USA. EM rodney.cooper@ars.usda.gov RI Cooper, William/D-3205-2017 NR 16 TC 8 Z9 8 U1 0 U2 13 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 860 EP 865 DI 10.1603/EC11312 PG 6 WC Entomology SC Entomology GA 961NY UT WOS:000305473500014 PM 22812122 ER PT J AU Behrens, NS Zhu, JW Coats, JR AF Behrens, Nicholas S. Zhu, Junwei Coats, Joel R. TI Pan Trapping Soybean Aphids (Hemiptera: Aphididae) Using Attractants SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE soybean aphid; pan trap; sex pheromone; plant volatile; electroantennogram ID ALATE APHIDS; GLYCINES MATSUMURA; NORTH-AMERICA; MOSAIC-VIRUS; HOMOPTERA; MANAGEMENT; TRAPS; COLOR AB Since its introduction into the United States in the past 10 yr, soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), has been a damaging pest to soybean, Glycine max (L.) Merrill. During 2008 and 2009, fields in central and north central Iowa experienced pockets of high soybean aphid populations. Electroantennograms have shown that soybean aphid alatae are capable of detecting host plant volatiles and sex pheromones. Here, we evaluated baited pan traps as a potential soybean aphid attractant. Yellow pan traps were placed in soybean fields after planting along with lures that contained plant volatiles and sex pheromones in 2008 or sex pheromones only in 2009. Pan trap contents were collected weekly, and plant counts also were conducted. Aphids were identified, and soybean aphids were counted to determine whether one chemical lure was more attractive to spring migrants than other lures. In both years, soybean aphids collected in pan traps with lures were not significantly different from the other products tested. C1 [Behrens, Nicholas S.; Coats, Joel R.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Zhu, Junwei] Univ Nebraska, USDA ARS, AMRU, Lincoln, NE 68583 USA. RP Coats, JR (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM jcoats@iastate.edu OI Zhu, Junwei/0000-0001-9846-9656 FU Iowa Soybean Association FX We thank landowners Dean Coleman, Bill Howard, and Chris Carlson for field access and the undergraduate students that assisted in this project. We also thank the two reviewers for comments on an earlier version this manuscript. This is a journal article of the Iowa Agriculture Experiment Station, project 5075. This work was funded by the Iowa Soybean Association. NR 17 TC 0 Z9 0 U1 2 U2 16 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 890 EP 895 DI 10.1603/EC11102 PG 6 WC Entomology SC Entomology GA 961NY UT WOS:000305473500019 PM 22812127 ER PT J AU Brewer, MJ Anderson, DJ Armstrong, JS Villanueva, RT AF Brewer, Michael J. Anderson, Darwin J. Armstrong, J. Scott Villanueva, Raul T. TI Sampling Strategies for Square and Boll-Feeding Plant Bugs (Hemiptera: Miridae) Occurring on Cotton SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE cotton fleahopper; Pseudatomoscelis seriatus; verde plant bug; Creontiades signatus; pest sampling ID PSEUDATOMOSCELIS-SERIATUS; SHAKE-BUCKET; PENTATOMIDAE; FLEAHOPPER AB Sampling methods for square and boll-feeding plant bugs (Hemiptera: Miridae) occurring on cotton, Gossypium hirsutum L., were compared with the intent to assess if one approach was viable for two species occurring from early-season squaring to late bloom in 25 fields located along the coastal cotton growing region of south Texas. Cotton fleaphopper, Pseudatomoscelis seriatus (Reuter), damages squares early-season and dominated collections using five sampling methods (approximate to 99% of insects collected). A major species composition shift occurred beginning at peak bloom in coastal fields, when verde plant bug, Creontiades signatus Distant, represented 55-65% of collections. Significantly more cotton fleahoppers were captured by experienced samplers with the beat bucket and sweep net than with the other methods (30-100% more). There were more than twice as many verde plant bugs captured by experienced and inexperienced samplers with the beat bucket and sweep net than captured with the KISS and visual methods. Using a beat bucket or sweep net reduced sampling time compared with the visual method for the experienced samplers. For both species, comparing regressions of beat bucket-based counts to counts from the traditional visual method across nine cultivar and water regime combinations resulted in only one combination differing from the rest, suggesting broad applicability and ability to translate established visual-based economic thresholds to beat bucket-based thresholds. In a first look at sample size considerations, 40 plants (four 10-plant samples) per field site was no more variable than variation associated with larger sample sizes. Overall, the beat bucket is much more effective in sampling for cotton fleahopper and verde plant bug than the traditional visual method, it is more suited to cotton fleahopper sampling early-season when plants are small, it transitions well to sample for verde plant bug during bloom, and it performs well under a variety of soil moisture conditions and cultivar selections. C1 [Brewer, Michael J.; Anderson, Darwin J.] Texas AgriLife Res, Corpus Christi, TX 78406 USA. [Brewer, Michael J.; Anderson, Darwin J.] Texas AgriLife Res & Extens Ctr Corpus Christi, Dept Entomol, Corpus Christi, TX 78406 USA. [Armstrong, J. Scott] USDA ARS, Beneficial Insect Res Unit, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Villanueva, Raul T.] Texas AgriLife Extens, Weslaco, TX 78596 USA. [Villanueva, Raul T.] Texas AgriLife Res & Extens Ctr Weslaco, Dept Entomol, Weslaco, TX 78596 USA. RP Brewer, MJ (reprint author), Texas AgriLife Res, 10345 State Hwy 44, Corpus Christi, TX 78406 USA. EM mjbrewer@ag.tamu.edu FU Texas State Support Committee [11-845TX] FX We thank J. Martinez, E. Rodriquez, M. Bloemer, and C. Farias for assistance in insect field sampling. Thanks to L. Hutchins, J. Norman, S. Hopkins, M. Treacy, J. Trolinger, and S. Biles for identifying sucking-bug infested fields, and we thank field owners (D. Mayo, M. Mutchler, R. Neiman, C. Neiman, D. Nunley, S. Simmons, L. Simmons, B. Simpson, T. Ulhorn, and M. Yeary) for allowing field access. Two anonymous reviews were very helpful in improving this paper. This work was partially supported by a Texas State Support Committee award (11-845TX) to M.J.B. and J.S.A. NR 27 TC 4 Z9 4 U1 0 U2 12 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 896 EP 905 DI 10.1603/EC12109 PG 10 WC Entomology SC Entomology GA 961NY UT WOS:000305473500020 PM 22812128 ER PT J AU Allison, JD McKenney, JL Miller, DR Gimmel, ML AF Allison, Jeremy D. McKenney, Jessica L. Miller, Daniel R. Gimmel, Matthew L. TI Role of Ipsdienol, Ipsenol, and cis-Verbenol in Chemical Ecology of Ips avulsus, Ips calligraphus, and Ips grandicollis (Coleoptera: Curculionidae: Scolytinae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Ips; aggregation pheromone; reproductive isolation; Scolytidae ID BARK BEETLES COLEOPTERA; INTERSPECIFIC BEHAVIORAL CHEMICALS; HOST SELECTION; PINE ENGRAVERS; UNITED-STATES; AGGREGATION; PHEROMONES; COLONIZATION; ATTRACTION; EICHHOFF AB Stressed or damaged pine (Pinus sp.) trees in the southeastern United States are often colonized simultaneously by three southern Ips species (Coleoptera: Curculionidae: Scolytinae): small southern pine engraver, Ips avulsus (Eichhoff); sixspined ips, Ips calligraphus (Germar); and eastern fivespined ips, Ips grandicollis (Eichhoff). All three species mediate colonization of host material with volatile pheromones. All of the southern Ips produce cis-verbenol, and either ipsdienol or ipsenol, and electrophysiological studies have demonstrated that all three southern Ips are able to detect all three compounds. This study examined the role of ipsdienol, ipsenol, and cis-verbenol in the chemical ecology of the southern Ips in Georgia and Louisiana. The most attractive blends of pheromones, with the fewest number of components, were ipsdienol plus ipsenol for I. avulsus, cis-verbenol plus ipsdienol for I. calligraphus, and either cis-verbenol plus ipsenol or ipsdienol plus ipsenol for I. grandicollis. Cross-attraction of I. grandicollis to the pheromone blend most attractive to I. avulsus was observed. Although the presence of heterospecific pheromone reduced the catches of all three species (i.e., the tertiary blend captured fewer beetles than the most attractive binary blends) in both states (significantly in two cases), high numbers of all three species were still captured in traps baited with all three compounds. These results suggest that the pheromones cis-verbenol, ipsdienol, and ipsenol can be combined for monitoring all three species of the southern Ips simultaneously. C1 [Allison, Jeremy D.; McKenney, Jessica L.; Gimmel, Matthew L.] LSU AgCtr, Dept Entomol, Baton Rouge, LA 70803 USA. [Miller, Daniel R.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. RP Allison, JD (reprint author), Nat Resources Canada, Great Lakes Forestry Ctr, 1219 Queen St E, Sault Ste Marie, ON, Canada. EM jeremy.allison@nrcan.gc.ca RI Gimmel, Matthew/F-3389-2013 FU LSU AgCenter; USDA-Forest Service FX We thank C. M. Crowe for field assistance and Dearl Sanders (Bob R. Jones-Idlewild Research Station, LSU AgCenter) and the staff of the Oconee National Forest for assistance and access to field sites. We thank B. L. Strom and B. T. Sullivan for critical review of the original manuscript. Funding for this research was provided by the LSU AgCenter and USDA-Forest Service. NR 41 TC 8 Z9 8 U1 2 U2 18 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 923 EP 929 DI 10.1603/EC12078 PG 7 WC Entomology SC Entomology GA 961NY UT WOS:000305473500023 PM 22812131 ER PT J AU Gassmann, AJ Hannon, ER Sisterson, MS Stock, SP Carriere, Y Tabashnik, BE AF Gassmann, Aaron J. Hannon, Eugene R. Sisterson, Mark S. Stock, S. Patricia Carriere, Yves Tabashnik, Bruce E. TI Effects of Entomopathogenic Nematodes on Evolution of Pink Bollworm Resistance to Bacillus thuringiensis Toxin Cry1Ac SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE entomopathogenic nematode; genetically modified crop; meta-analysis; Pectinophora gossypiella; tritrophic interaction ID FITNESS COSTS; INSECT RESISTANCE; TRANSGENIC CROPS; BT COTTON; FIELD-RESISTANCE; MOTH EMERGENCE; HOST-PLANT; LEPIDOPTERA; GELECHIIDAE; METAANALYSIS AB The evolution of resistance by pests can reduce the efficacy of transgenic crops that produce insecticidal toxins from Bacillus thuringiensis (Bt). However, fitness costs may act to delay pest resistance to Bt toxins. Meta-analysis of results from four previous studies revealed that the entomopathogenic nematode Steinernema riobrave (Rhabditida: Steinernematidae) imposed a 20% fitness cost for larvae of pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), that were homozygous for resistance to Bt toxin Cry1Ac, but no significant fitness cost was detected for heterozygotes. We conducted greenhouse and laboratory selection experiments to determine whether S. riobrave would delay the evolution of pink bollworm resistance to Cry1Ac. We mimicked the high dose/refuge scenario in the greenhouse with Bt cotton (Gossypium hirsutum L.) plants and refuges of non-Bt cotton plants, and in the laboratory with diet containing Cry1Ac and refuges of untreated diet. In both experiments, half of the replicates were exposed to S. riobrave and half were not. In the greenhouse, S. riobrave did not delay resistance. In the laboratory, S. riobrave delayed resistance after two generations but not after four generations. Simulation modeling showed that an initial resistance allele frequency >0.015 and population bottlenecks can diminish or eliminate the resistance-delaying effects of fitness costs. We hypothesize that these factors may have reduced the resistance-delaying effects of S. riobrave in the selection experiments. The experimental and modeling results suggest that entomopathogenic nematodes could slow the evolution of pest resistance to Bt crops, but only under some conditions. C1 [Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Hannon, Eugene R.; Stock, S. Patricia; Carriere, Yves; Tabashnik, Bruce E.] Univ Arizona, Dept Entomol, Tucson, AZ 85705 USA. [Sisterson, Mark S.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Gassmann, AJ (reprint author), Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. EM aaronjg@iastate.edu FU U.S. Department of Agriculture through the National Research Initiative [2007-35302-18415] FX We thank Ana Romo, Alex Yelich, Christa Ellers-Kirk, Ginny Harpold, and Gopalan Unnithan for laboratory assistance. This research was supported by a grant from the U.S. Department of Agriculture through the National Research Initiative 2007-35302-18415. NR 65 TC 3 Z9 3 U1 0 U2 22 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 994 EP 1005 DI 10.1603/EC11376 PG 12 WC Entomology SC Entomology GA 961NY UT WOS:000305473500033 PM 22812141 ER PT J AU Puterka, GJ Hammon, RW Burd, JD Peairs, FB Randolph, TL Cooper, WR AF Puterka, G. J. Hammon, R. W. Burd, J. D. Peairs, F. B. Randolph, T. L. Cooper, W. R. TI Cyclical Parthenogenetic Reproduction in the Russian Wheat Aphid (Hemiptera: Aphididae) in the United States: Sexual Reproduction and Its Outcome on Biotypic Diversity SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Diuraphis noxia; plant resistance; wheat; genetic diversity; adaptation ID DIURAPHIS-NOXIA KURDJ.; GENETIC-VARIATION; BARLEY GERMPLASM; NORTH-AMERICA; HOMOPTERA; POPULATIONS; RESISTANCE; IDENTIFICATION; INHERITANCE; STARS-9577B AB In 1986, the Russian wheat aphid, Diuraphis noxia (Kurdjumov) (Hemiptera: Aphididae), became an invasive species of United States. Nearly 20 yr later, new biotypes appeared that were capable of overcoming most sources of resistance and became a renewed threat to wheat, Triticum aestivum L., production. Cyclical (CP) and obligate (OP) parthenogenesis enables aphids to both adapt to changing environments and exploit host resources. We documented these forms of reproduction for Russian wheat aphid in wheat and wild grasses in the Central Great Plains and Rocky Mountain regions during falls 2004-2009. Colonies from sample sites also were held under unheated greenhouse conditions and observed for the presence of sexual morphs and eggs through the winter. Russian wheat aphid populations were mainly OP and attempted to overwinter as adults, regardless of region sampled. A few populations contained oviparae but no males (gynocyclic) and were not specific to any particular region. Observation of the Russian wheat aphid colonies under greenhouse conditions failed to produce males or eggs. In spring 2007, CP was confirmed in a small population of Russian wheat aphid that eclosed from eggs (fundatricies) on wild grasses and wheat near Dove Creek, CO, in the Colorado Plateau region where other aphid species undergo CP. Lineages from ninety-three fundatricies were screened against 16 resistant and susceptible cereal entries to determine their biotypic classification. A high degree of biotypic diversity (41.4%) was detected in this population. Although CP was a rare in Russian wheat aphid populations, genetic recombination during the sexual cycle creates new biotypes and can have significant effects on population genetics. C1 [Puterka, G. J.; Burd, J. D.] USDA ARS, Plant Sci Res Lab, Stillwater, OK 74074 USA. [Hammon, R. W.] Colorado State Univ, Tri River Area Extens, Grand Junction, CO 81502 USA. [Peairs, F. B.; Randolph, T. L.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Cooper, W. R.] USDA ARS WICS, Shafter, CA 93263 USA. RP Puterka, GJ (reprint author), USDA ARS, Plant Sci Res Lab, 1301 N Western, Stillwater, OK 74074 USA. EM gary.puterka@ars.usda.gov RI Cooper, William/D-3205-2017 FU Colorado State University [6217-22000-015-07S] FX We thank Keith Mirkes (USDA-ARS, Stillwater, OK) for technical support in all phases of aphid colony maintenance and screening operations. We also acknowledge Tom Hooten (Colorado State University Extension Service, Cortez, CO) and Steve Parr (UCEPC) for collecting and mailing aphid samples during the course of this project. This project, in part, was conducted under a specific cooperative agreement between USDA, ARS, Stillwater, OK, and Colorado State University under project 6217-22000-015-07S. NR 38 TC 6 Z9 6 U1 4 U2 21 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-0493 J9 J ECON ENTOMOL JI J. Econ. Entomol. PD JUN PY 2012 VL 105 IS 3 BP 1057 EP 1068 DI 10.1603/EC11338 PG 12 WC Entomology SC Entomology GA 961NY UT WOS:000305473500040 PM 22812148 ER PT J AU Kim, SD De La Fuente, L Weller, DM Thomashow, LS AF Kim, Sang-Dal De La Fuente, Leonardo Weller, David M. Thomashow, Linda S. TI Colonizing Ability of Pseudomonas fluorescens 2112, Among Collections of 2,4-Diacetylphloroglucinol-Producing Pseudomonas fluorescens spp. in Pea Rhizosphere SO JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Pseudomonas fluorescens; 2,4-diacetylphloroglucinol (2,4-DAPG); colonization; plant growth-promoting rhizobacteria (PGPR) ID POPULATION-DYNAMICS; COLONIZATION; BACTERIA; STRAINS; WHEAT; GENOTYPES; ROOTS AB Pseudomonas fluorescens 2112, isolated in Korea as an indigenous antagonistic bacteria, can produce 2,4-diacetylphloroglucinol (2,4-DAPG) and the siderophore pyoveridin(2112) for the control of phytophthora blight of red-pepper. P fluorescens 2112 was classified into a new genotype C among the 17 genotypes of 2,4-DAPG producers, by phID restriction fragment length polymorphism (RFLP). The colonizing ability of P fluorescens 2112 in pea rhizosphere was equal to the well-known pea colonizers, P fluorescens Q8rl (genotype D) and MVP1-4 (genotype P), after 6 cycling cultivations for 18 weeks. Four tested 2,4-DAPG-producing Pseudomonas spp. could colonize with about a 96% dominance ratio against total bacteria in pea rhizosphere. The strain P fluorescens 2112 was as good a colonizer as other Pseudomonas spp. genotypes in pea plant growth-promoting rhizobacteria. C1 [Kim, Sang-Dal] Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea. [De La Fuente, Leonardo] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. [Weller, David M.; Thomashow, Linda S.] Washington State Univ, Root Dis & Biol Control Res Unit, USDA ARS, Dept Plant Pathol, Pullman, WA 99164 USA. RP Kim, SD (reprint author), Yeungnam Univ, Sch Biotechnol, Gyongsan 712749, South Korea. EM sdkim@ynu.ac.kr RI De La Fuente, Leonardo/A-2012-2013 FU Yeungnam University, South Korea; USDA-ARS, Washington State University, USA FX This research was supported by Yeungnam University, South Korea, and USDA-ARS, Washington State University, USA. NR 23 TC 1 Z9 2 U1 3 U2 11 PU KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY PI SEOUL PA KOREA SCI TECHNOL CENTER #507, 635-4 YEOGSAM-DONG, KANGNAM-GU, SEOUL 135-703, SOUTH KOREA SN 1017-7825 J9 J MICROBIOL BIOTECHN JI J. Microbiol. Biotechnol. PD JUN PY 2012 VL 22 IS 6 BP 763 EP 770 DI 10.4014/jmb.1112.12039 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 966NZ UT WOS:000305846000005 PM 22573152 ER PT J AU Choi, YE Butchko, RAE Shim, WB AF Choi, Yoon-E Butchko, Robert A. E. Shim, Won-Bo TI Proteomic Comparison of Gibberella moniliformis in Limited-Nitrogen (Fumonisin-Inducing) and Excess-Nitrogen (Fumonisin-Repressing) Conditions SO JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Fusarium verticillioides; nitrogen metabolite repression; fumonisin regulation; proteomics; gene expression ID FUSARIUM-VERTICILLIOIDES; B-1 BIOSYNTHESIS; GENE; EXPRESSION; PERSPECTIVE; MYCOTOXINS; FUJIKUROI; SUBUNIT; CLUSTER; CANCER AB The maize pathogen Gibberella moniliformis produces fumonisins, a group of mycotoxins associated with several disorders in animals and humans, including cancer. The current focus of our research is to understand the regulatory mechanisms involved in fumonisin biosynthesis. In this study, we employed a proteomics approach to identify novel genes involved in the fumonisin biosynthesis under nitrogen stress. The combination of genome sequence, mutant strains, EST database, microarrays, and proteomics offers an opportunity to advance our understanding of this process. We investigated the response of the G moniliformis proteome in limited nitrogen (NO, fumonisin-inducing) and excess nitrogen (N+, fumonisin-repressing) conditions by one- and two-dimensional electrophoresis. We selected 11 differentially expressed proteins, six from limited nitrogen conditions and five from excess nitrogen conditions, and determined the sequences by peptide mass fmgerprinting and MS/MS spectrophotometry. Subsequently, we identified the EST sequences corresponding to the proteins and studied their expression profiles in different culture conditions. Through the comparative analysis of gene and protein expression data, we identified three candidate genes for functional analysis and our results provided valuable clues regarding the regulatory mechanisms of fumonisin biosynthesis. C1 [Choi, Yoon-E] Chonbuk Natl Univ, LED Agribio Fusion Technol Res Ctr, Iksansi 570752, South Korea. [Butchko, Robert A. E.] USDA ARS, Bacterial Foodborne Pathogens & Mycotoxin Res Uni, Peoria, IL 61604 USA. [Shim, Won-Bo] Texas A&M Univ, Dept Plant Pathol & Microbiol, Program Biol Filamentous Fungi, College Stn, TX 77843 USA. RP Choi, YE (reprint author), Chonbuk Natl Univ, LED Agribio Fusion Technol Res Ctr, Iksansi 570752, South Korea. EM yechoi@jbnu.ac.kr FU TAMU/TAES; TAMU International FX We thank the scientists at In2Gen Inc. (Seoul, Korea) for providing excellent proteome analysis service. We also thank Drs. Clint Magill and Brian Shaw at Texas A&M University for critical reading of this manuscript. Financial support was provided in part by TAMU/TAES Faculty Start-up Fund and by the TAMU International Research Travel Assistance Grant. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 28 TC 1 Z9 1 U1 2 U2 5 PU KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY PI SEOUL PA KOREA SCI TECHNOL CENTER #507, 635-4 YEOGSAM-DONG, KANGNAM-GU, SEOUL 135-703, SOUTH KOREA SN 1017-7825 J9 J MICROBIOL BIOTECHN JI J. Microbiol. Biotechnol. PD JUN PY 2012 VL 22 IS 6 BP 780 EP 787 DI 10.4014/jmb.1111.11044 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 966NZ UT WOS:000305846000007 PM 22573154 ER PT J AU Turcotte, RM Elliott, TR Fajvan, MA Park, YL Snider, DA Tobin, PC AF Turcotte, Richard M. Elliott, Thomas R. Fajvan, Mary Ann Park, Yong-Lak Snider, Daniel A. Tobin, Patrick C. TI Effects of Ice Storm Damage on Hardwood Survival and Growth in Ohio SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE ice storm; crown damage; epicormic sprouts ID SOUTHWESTERN VIRGINIA; FOREST; TREES; EVENTS AB In 2003, an ice storm occurred across four Mid-Atlantic states. This study investigated the effects of the ice-storm damage on growth and mortality of five tree species (Acer rubrum, Acer saccharum, Quercus alba, Quercus prinus, and Quercus rubra) from three forest stands in the Wayne National Forest in Ohio. We remeasured the same trees five times between 2003 and 2009. Logistic regression analysis of mortality revealed significant (P < 0.05) effects of tree species, crown damage, and size class. We detected a significant effect of crown class on epicormic branching (P < 0.01), and saplings and poles as a group had significantly less dieback than sawtimber (P < 0.01). Dieback also varied according to crown class, with codominant and dominant trees having significantly (P < 0.05) more dieback than both intermediate and overtopped trees. We detected a significant (1) < 0.01) reduction in diameter growth when >33% of a tree's crown was damaged. The results of this study will help forest managers to make decisions about salvage, sanitation, and forest rehabilitation efforts following ice storm damage. C1 [Turcotte, Richard M.; Snider, Daniel A.] US Forest Serv, Morgantown Field Off, Morgantown, WV 26505 USA. [Elliott, Thomas R.] Anim Plant Hlth Inspect Serv, USDA, Morgantown, WV 26505 USA. [Fajvan, Mary Ann; Tobin, Patrick C.] US Forest Serv, No Res Stn, Morgantown, WV 26505 USA. [Park, Yong-Lak] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. RP Turcotte, RM (reprint author), US Forest Serv, Morgantown Field Off, 180 Canfield St, Morgantown, WV 26505 USA. EM rturcotte@fs.fed.us RI Park, Yong-Lak/F-4309-2012 NR 55 TC 6 Z9 7 U1 0 U2 7 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 J9 NORTH J APPL FOR JI North. J. Appl. For. PD JUN PY 2012 VL 29 IS 2 BP 53 EP 59 DI 10.5849/njaf.10-053 PG 7 WC Forestry SC Forestry GA 964WW UT WOS:000305728400001 ER PT J AU Anderson, NM Germain, RH Hall, MH AF Anderson, Nathaniel M. Germain, Rene H. Hall, Myrna H. TI An Assessment of Forest Cover and Impervious Surface Area on Family Forests in the New York City Watershed SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE parcelization; land cover; development; impervious surface; watershed management ID LAND-USE; NEW-JERSEY; PARCELIZATION; QUALITY; IMPACT; USA; INDICATORS; OWNERSHIP; NITROGEN; STREAMS AB Between 1984 and 2000, the parcelization of family forests in the New York City Watershed caused a decline in average parcel size from 19 to 16 ac. However, little is known about the timing and intensity of development on subdivided parcels, which has the potential to negatively affect water quality by increasing nonpoint source pollution associated with nutrient runoff and erosion intensified by increased impervious surface area. Using a combination of field measurements and analysis of digital orthoimagery, this study quantified forest cover and impervious surface area on new parcels resulting from subdivision and compared subdivided parcels to intact parcels. Measurements of buildings, driveways, and other features show that by 2005, parcels subdivided between 1984 and 2000 were developed to nearly the same intensity as intact parcels, with 68% of parcels classified as developed. Results indicate that residential development on subdivided parcels has added more than 640 ac of impervious surfaces on family forest since 1984, apparently without being accompanied by observable net reductions in forest cover at the landscape level. These trends have important implications for public policy intended to maintain a forested watershed and prevent the degradation of drinking water quality from nonpoint source pollution associated with development. C1 [Anderson, Nathaniel M.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59807 USA. [Germain, Rene H.] SUNY Coll Environm Sci & Forestry, Dept Forest & Nat Resources Management, Syracuse, NY 13210 USA. [Hall, Myrna H.] SUNY Coll Environm Sci & Forestry, Dept Environm Studies, Syracuse, NY 13210 USA. RP Anderson, NM (reprint author), US Forest Serv, Rocky Mt Res Stn, 200 E Broadway, Missoula, MT 59807 USA. EM nathanielmanderson@fs.fed.us FU McIntire-Stennis Cooperative Forestry Research Program; Edna Bailey Sussman Foundation; New York Department of Environmental Conservation, Division of Water FX Nathaniel M. Anderson (nathanielmanderson@fs.fed.us), US Forest Service, Rocky Mountain Research Station, US Forest Service, Rocky Mountain Research Station, 200 East Broadway, Missoula, MT 59807. Rene H. Germain, State University of New York, College of Environmental Science and Forestry, Department of Forest and Natural Resources Management, 1 Forestry Drive, Syracuse, NY 13210 Myrna H. Hall, State University of New York, College of Environmental Science and Forestry, Department of Environmental Studies, 1 Forestry Drive, Syracuse, NY 13210. The authors acknowledge funding from the McIntire-Stennis Cooperative Forestry Research Program, the Edna Bailey Sussman Foundation, and the New York Department of Environmental Conservation, Division of Water. NR 41 TC 0 Z9 0 U1 2 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 J9 NORTH J APPL FOR JI North. J. Appl. For. PD JUN PY 2012 VL 29 IS 2 BP 67 EP 73 DI 10.5849/njaf.11-009 PG 7 WC Forestry SC Forestry GA 964WW UT WOS:000305728400003 ER PT J AU Caron, JA Germain, RH Anderson, NM AF Caron, Jennifer A. Germain, Rene H. Anderson, Nathaniel M. TI Parcelization and Land Use: A Case Study in the New York City Watershed SO NORTHERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE watershed management; ownership fragmentation; family forests; forest management ID FAMILY FOREST OWNERS; MANAGEMENT AB Over 75% of the New York City Watershed is forested, and the majority of the land is owned by family forest owners. Ownership fragmentation and development may impact both the working forested landscape and water quality. We surveyed the owners of intact and subdivided family forest parcels across various parcel sizes to gauge their awareness of forest management practices and to assess the potential property-level impacts of their activities on water quality. To support the landowner survey, we used field data on forest stacking and timber quality gathered at each property. Results indicate that owners of large parcels (>50 ac) had significantly higher water quality awareness scores than did owners of small parcels and were more likely to have a written forest management plan. Full-time resident owners were more likely to engage in practices that may negatively impact water quality more directly, such as adding structures and a driveway, which increases impervious surface area and associated runoff, and using additives on their lawns, which can add nutrients and chemicals to surface water. C1 [Caron, Jennifer A.] Calif Publ Util Commiss, San Francisco, CA 94102 USA. [Germain, Rene H.] SUNY ESF FNRM, Syracuse, NY 13210 USA. [Anderson, Nathaniel M.] US Forest Serv, USDA, Missoula, MT 59807 USA. [Anderson, Nathaniel M.] Univ Montana, Missoula, MT 59812 USA. RP Caron, JA (reprint author), Calif Publ Util Commiss, San Francisco, CA 94102 USA. EM jennifercaron8@gmail.com; rhgermai@esf.edu; nathanielanderson@fs.fed.us FU McIntire-Stennis program FX Jennifer A. Caron (jennifercaron8@gmail.com), California Public Utilities Commission, San Francisco, CA 94102. Rene H. Germain (rhgermai@esf.edu), SUNY-ESF FNRM, 316 Bray Hall, 1 Forestry Drive, Syracuse, NY 13210. Nathaniel M. Anderson (nathanielanderson@fs.fed.us), USDA Forest Service, Missoula, MT 59807. University of Montana. This study was partially funded by the McIntire-Stennis program. NR 17 TC 6 Z9 7 U1 1 U2 8 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0742-6348 EI 1938-3762 J9 NORTH J APPL FOR JI North. J. Appl. For. PD JUN PY 2012 VL 29 IS 2 BP 74 EP 80 DI 10.5849/njaf.10-038 PG 7 WC Forestry SC Forestry GA 964WW UT WOS:000305728400004 ER PT J AU Woodley, NE AF Woodley, Norman E. TI Brianmyia stuckenbergi, a new genus and species of Prosopochrysini from South Africa (Diptera: Stratiomyidae) SO AFRICAN INVERTEBRATES LA English DT Article DE Stratiomyidae; Brianmyia; soldier flies; Afrotropical; South Africa; Drakensberg; new genus; new species AB A new genus and species of Stratiomyidae, Brianmyia stuckenbergi gen. et sp. n., are described from the Drakensberg, KwaZulu-Natal, South Africa. The new genus is placed in the tribe Prosopochrysini of the subfamily Stratiomyinae and is the first genus and species of this tribe known from southern Africa. C1 ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst NHB 168, Washington, DC 20013 USA. RP Woodley, NE (reprint author), ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst NHB 168, POB 37012, Washington, DC 20013 USA. EM norman.woodley@ars.usda.gov RI Woodley, Norman/M-6160-2014 NR 7 TC 1 Z9 1 U1 0 U2 0 PU COUNCIL NATAL MUSEUM PI PIETERMARITZBURG PA PRIVATE BAG 9070, PIETERMARITZBURG, KWAZULU-NATAL 00000, SOUTH AFRICA SN 1681-5556 J9 AFR INVERTEBR JI Afr. Invertebr. PD JUN PY 2012 VL 53 IS 1 BP 369 EP 374 PG 6 WC Entomology; Paleontology; Zoology SC Entomology; Paleontology; Zoology GA 961IE UT WOS:000305457100021 ER PT J AU Williamson, GB Wiemann, MC Geaghan, JP AF Williamson, G. Bruce Wiemann, Michael C. Geaghan, James P. TI RADIAL WOOD ALLOCATION IN SCHIZOLOBIUM PARAHYBA SO AMERICAN JOURNAL OF BOTANY LA English DT Article DE age-dependence; pioneers; radial variation; Schizolobium parahyba; size-dependence; specific gravity; succession; wood allocation; wood density ID RAIN-FOREST TREES; TROPICAL FOREST; NEOTROPICAL FORESTS; FUNCTIONAL TRAITS; MOIST-FOREST; TRADE-OFFS; GRAVITY; DENSITY; GROWTH; LIGHT AB Premise of the study: Pioneer species of tropical trees allocate wood specific gravity (SG) differently across the radius. Some species exhibit relatively uniform, low SG wood, whereas many others exhibit linear increases in SG across the radius. Here, we measured changes in SG across the radius of Schizolobium parahyba (Fabaceae-Caesalpinioideae), a wide-ranging, neotropical pioneer, used extensively in land reclamation and forest restoration in Brazil. Methods: Pith-to-bark radial wood cores were extracted with increment borers from 42 trees at five sites, in Central and South America. Cores were cut into 1-cm segments whose specific gravities were determined and analyzed via linear and nonlinear regression. Wood specific gravity, very low initially at 0.15-0.20, doubled or tripled across the tree radius to 0.45-0.65 for large adults. Key results: Unlike linear increases in other tropical pioneers, the increases in Schizolobium were nonlinear (convex up). At one site with even-aged trees, the magnitude of the radial increase was similar in all trees, despite a 4-fold difference in diameter among trees, implying that the radial increases in Schizolobium were regulated by tree age, not by tree size. Conclusions: This unique pattern of development should provide an extended period of growth when SG is low, facilitating hyper-extension of the bole, at some risk of structural failure. Later in growth, the SG rate of increase accelerates, reinforcing what was a precarious bole. Overall, these results suggest a third model for xylem allocation in tropical trees, a model that may be associated with monopodial stem development and limited life span. C1 [Williamson, G. Bruce] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA. [Wiemann, Michael C.] US Forest Serv, Ctr Wood Anat Res, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Williamson, G. Bruce] Louisiana State Univ, Dept Expt Stat, Baton Rouge, LA 70803 USA. EM btwill@lsu.edu FU Organization for Tropical Studies; Brazilian Fulbright Association; UNICAMP; CAPES; Jardim Botanico de Sao Paulo; Companhia Vale do Rio Doce; Biological Dynamics of Forest Fragments Project; US National Science Foundation [DEB-0639114, DEB-1147434] FX Field research in Costa Rica was supported by the Organization for Tropical Studies, and in Brazil by the Brazilian Fulbright Association, UNICAMP, CAPES, Jardim Botanico de Sao Paulo, Companhia Vale do Rio Doce, Biological Dynamics of Forest Fragments Project, and US National Science Foundation grants to GBW (DEB-0639114 and DEB-1147434). This is publication 593 in the Technical Series of the Biological Dynamics of Forest Fragments Project BDFFP-INPA-SI. NR 70 TC 2 Z9 2 U1 1 U2 10 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 0002-9122 J9 AM J BOT JI Am. J. Bot. PD JUN PY 2012 VL 99 IS 6 BP 1010 EP 1019 DI 10.3732/ajb.1100516 PG 10 WC Plant Sciences SC Plant Sciences GA 960ZB UT WOS:000305431300015 PM 22575368 ER PT J AU Lin, LG Pang, WJ Chen, KY Wang, F Gengler, J Sun, YX Tong, Q AF Lin, Ligen Pang, Weijun Chen, Keyun Wang, Fei Gengler, Jon Sun, Yuxiang Tong, Qiang TI Adipocyte expression of PU.1 transcription factor causes insulin resistance through upregulation of inflammatory cytokine gene expression and ROS production SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE reactive oxygen species; nicotinamide adenine dinucleotide phosphate oxidase; tumor necrosis factor-alpha; interleukin-1 beta; c-Jun NH2-terminal kinase; lipolysis ID NECROSIS-FACTOR-ALPHA; ACTIVATED SIGNALING PATHWAYS; INCREASED OXIDATIVE STRESS; BETA-CELL DYSFUNCTION; PROTEIN-KINASE-C; NADPH OXIDASE; TNF-ALPHA; 3T3-L1 ADIPOCYTES; HYDROGEN-PEROXIDE; ADIPOSE-TISSUE AB We have reported previously that ETS family transcription factor PU.1 is expressed in mature adipocytes of white adipose tissue. PU.1 expression is increased greatly in mouse models of genetic or diet-induced tissue. PU.1, we show that PU.1 expression is increased only in visceral but not subcutaneous adipose tissues of obese mice, and the adipocytes are responsible for this increase in PU.1 expression. To further address PU.1's physiological function in mature adipocytes, PU.1 was knocked down in 3T3-L1 cells using retroviral-mediated expression of PU.1-targeting shRNA. Consistent with previous findings that PU.1 regulates its target genes, such as NADPH oxidase subunits and proinflammatory cytokines in myeloid cells, the mRNA levels of proinflammatory cytokines (TNF alpha, IL-1 beta, and IL-6) and cytosolic components of NADPH oxidase (p47phox and p40phox) were down-regulated significantly in PU.1-silenced adipocytes. NADPH oxidase is a main source for reactive oxygen species (ROS) generation. Indeed, silencing PU.1 suppressed NADPH oxidase activity and attenuated ROS in basal or hydrogen peroxide-treated adipocytes.Silencing PU.1 in adipocytes suppressed JNK1 activation and IRS-1 phosphorylation at Ser(307). Consequently, PU.1 knockdown improved insulin signaling and increased glucose uptake in basal and insulin-stimulated conditions. Furthermore, knocking down PU.1 suppressed basal lipolysis but activated stimulated lipolysis.Collectively, these findings indicate that obesity induces PU.1 expression in adipocytes to upregulate the production of ROS and proinflammatory cytokines, both of which lead to JNK1 activation, insulin resistance, and dysregulation of lipolysis. Therefore, PU.1 might be a mediator for obesity-induced adipose inflammation and insulin resistance. C1 [Tong, Qiang] Baylor Coll Med, Childrens Nutr Res Ctr, Dept Med, Houston, TX 77030 USA. [Lin, Ligen; Pang, Weijun; Chen, Keyun; Wang, Fei; Gengler, Jon; Sun, Yuxiang; Tong, Qiang] ARS, USDA, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. [Gengler, Jon; Tong, Qiang] Baylor Coll Med, Dept Mol Physiol & Biophys, Houston, TX 77030 USA. [Pang, Weijun] NW Univ Agr & Forestry, Yangling, Shaanxi, Peoples R China. RP Tong, Q (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, Dept Med, 1100 Bates St, Houston, TX 77030 USA. EM qtong@bcm.edu FU US Department of Agriculture/Agricultural Research Service (USDA/ARS) [6250-51000-049, 6250-51000-055]; National Institute of Diabetes and Digestive and Kidney Diseases [DK-075978]; American Heart Association [12IRG9230004] FX This work was supported by a US Department of Agriculture/Agricultural Research Service (USDA/ARS) grant (6250-51000-049) and a National Institute of Diabetes and Digestive and Kidney Diseases grant (DK-075978) to Q. Tong. This study was also supported by USDA/ARS Grant 6250-51000-055 and American Heart Association Grant 12IRG9230004 to Y. Sun. NR 76 TC 10 Z9 13 U1 0 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUN PY 2012 VL 302 IS 12 BP E1550 EP E1559 DI 10.1152/ajpendo.00462.2011 PG 10 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 960XQ UT WOS:000305426900011 PM 22454293 ER PT J AU Judd, WS Melvin, NC Waselkov, K Kron, KA AF Judd, Walter S. Melvin, Norman C., III Waselkov, Katherine Kron, Kathleen A. TI Taxonomic revision of Eubotrys (Ericaceae, Gaultherieae) SO BRITTONIA LA English DT Article DE Eubotrys; Leucothoe; Gaultherieae; Ericaceae ID PHYLOGENETIC-RELATIONSHIPS; LYONIA ERICACEAE; SEQUENCE DATA; VACCINIOIDEAE; MATK; CLASSIFICATION; MONOGRAPH; NRITS; NDHF AB Eubotrys includes E. racemosa and E. recurva, and these species form a southern Appalachian - southeastern Coastal Plain species pair. Generic autapomorphies include deciduous leaves with unicellular hairs on the lamina both adaxially and abaxially, megagametophytes with antipodals dividing, and quickly deciduous inflorescence bracts that are photosynthetic and elongate but not equivalent to leaves. The Eubotrys clade is sister to Chamaedaphne, with which it shares the apomorphies of fall-formed inflorescences of the E. racemosa type, staminal filaments that are merely papillose, placentas with a bare spot adaxially, and campylotropous ovules. Eubotrys is thus generically distinct from LeucothoA << sensu stricto and Eubotryoides grayana. Species descriptions, nomenclatural information, specimen citations, and eco-geographical characterizations for these two species are presented, along with an identification key. C1 [Judd, Walter S.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Melvin, Norman C., III] USDA NRCS, Cent Natl Technol Support Ctr, FWFC, Ft Worth, TX 76115 USA. [Waselkov, Katherine] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Kron, Kathleen A.] Wake Forest Univ, Dept Biol, Winston Salem, NC 27109 USA. RP Judd, WS (reprint author), Univ Florida, Dept Biol, 220 Bartram Hall,POB 118525, Gainesville, FL 32611 USA. EM wjudd@botany.ufl.edu; Norman.Melvin@ftw.usda.gov; kewaselk@wustl.edu; kronka@wfu.edu FU Willard Sherman Turrell Herbarium Fund; Miami University Graduate School Achievement Fund; National Science Foundation [DEB-0717724, DEB-0717711]; Wake Forest University FX We thank the curators of A, AUA, DUKE, FLAS, FSU, GA, GH, LAF, LSU, MISS, MU, NCSC, NCU, NLU, NY, PH, TENN, TROY, UNA, and US for their loans of specimens, and Kent Perkins of the University of Florida Herbarium for assistance with specimen processing. Appreciation is extended to the staff of the Lloyd Library, Cincinnati, Ohio, for their help in procuring original works for most of the literature citations. We are grateful for the helpful comments of Daniel Atha and an anonymous reviewer. N.C.M. thanks the administrators of the Willard Sherman Turrell Herbarium Fund and the Miami University Graduate School Achievement Fund for their support, and this revision reflects, in part, work done as part of his doctoral dissertation. Finally, K.A.K. and W.S.J. acknowledge (and are grateful for) support from the National Science Foundation (grants: DEB-0717724, DEB-0717711) and Wake Forest University. NR 46 TC 2 Z9 2 U1 0 U2 8 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0007-196X J9 BRITTONIA JI Brittonia PD JUN PY 2012 VL 64 IS 2 BP 165 EP 178 DI 10.1007/s12228-011-9226-9 PG 14 WC Plant Sciences SC Plant Sciences GA 960RE UT WOS:000305405500011 ER PT J AU Shortle, WC Smith, KT Jellison, J Schilling, JS AF Shortle, Walter C. Smith, Kevin T. Jellison, Jody Schilling, Jonathan S. TI Potential of decaying wood to restore root-available base cations in depleted forest soils SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID NORTHEASTERN UNITED-STATES; RED SPRUCE; ACIDIC DEPOSITION; NEW-ZEALAND; BROWN-ROT; FIR WOOD; FUNGI; TRANSLOCATION; DECOMPOSITION; ALUMINUM AB The depletion of root-available Ca in northern forest soils exposed to decades of increased acid deposition adversely affects forest health and productivity. Laboratory studies indicated the potential of wood-decay fungi to restore lost Ca. This study presents changes in concentration of Ca, Mg, and K in sapwood of red spruce (Picea rubens Sarg.), red maple (Acer rubrum L.), eastern hemlock (Tsuga canadensis (L.) Carriere), and paper birch (Betula papyrifera Marshall) during the decay process at two experimental forests for 12 years and to compare concentrations of exchangeable Ca, Mg, and Al in decayed wood residues at 10 and 12 years with those in the forest floor. Significant loss of mass indicated by decreasing wood density occurred after 2-8 years in conifers and after only 2 years in hardwoods. A significant gain in wood K was observed at 2 years followed by a significant loss at 8 years. A negligible gain in Ca concentration occurred at 2 years and a substantial gain at 8 years. Observed changes in Mg concentration were variable. No significant difference in exchangeable Ca concentration was observed between decayed wood residue of spruce and maple and the forest floor. However, decayed wood residue had a much lower Al concentration and molar Al/Ca ratio, a condition characteristic of sites with high root-available Ca. C1 [Shortle, Walter C.; Smith, Kevin T.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Jellison, Jody] Virginia Tech, Virginia Agr Expt Stn, Blacksburg, VA 24061 USA. [Schilling, Jonathan S.] Univ Minnesota, Dept Bioprod & Biosyst Engn, Kaufert Lab 108, St Paul, MN 55108 USA. RP Shortle, WC (reprint author), US Forest Serv, USDA, No Res Stn, 271 Mast Rd, Durham, NH 03824 USA. EM wshortle@fs.fed.us FU Maine Agricultural and Forest Experiment Station FX We thank Andrea Ostrofsky, Joan Perkins, and Kenneth Dudzik for their invaluable technical assistance and Gregory Lawrence for providing soil data. We thank the Bartlett and Penobscot experimental forests of the Northern Research Station, USDA Forest Service, for access to the study locations. We acknowledge the support of the Maine Agricultural and Forest Experiment Station. NR 41 TC 8 Z9 8 U1 0 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2012 VL 42 IS 6 BP 1015 EP 1024 DI 10.1139/X2012-056 PG 10 WC Forestry SC Forestry GA 957IQ UT WOS:000305155500003 ER PT J AU Rebbeck, J Scherzer, A Gottschalk, K AF Rebbeck, Joanne Scherzer, Amy Gottschalk, Kurt TI Do chestnut, northern red, and white oak germinant seedlings respond similarly to light treatments? II. Gas exchange and chlorophyll responses SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID REPEATED PRESCRIBED FIRES; YELLOW-POPLAR SEEDLINGS; MAPLE ACER-SACCHARUM; SHADE-TOLERANCE; QUERCUS-RUBRA; SUCCESSIONAL STATUS; LEAF CHLOROPHYLL; ARTIFICIAL SHADE; TREE SEEDLINGS; CARBON-DIOXIDE AB Understanding differences in physiological and growth strategies in low-light environments among upland oak species may help managers address the challenges of oaks' poor regeneration. Gas exchange and chlorophyll content were measured for northern red oak (Quercus rubra L.), chestnut oak (Quercus prinus L.), and white oak (Quercus alba L.) germinants grown at 25%, 18%, and 6% of full sun in one of two native forest soil mixes for two summers. Northern red and chestnut oak photosynthesis at saturating light (A(max, mass)) increased by 23%-36% as light levels increased from 6% to 25% of full sun, while white oak A(max, mass) declined by 20% and plateaued at 18% of full sun. White oak light compensation point is representative of deep shade (7.2 mu mol.m(-2).s(-1)), while northern red and chestnut oak averaged 17.8 mu mol.m(-2).s(-1). Total chlorophyll content increased as light levels decreased for all species. Of the three species, the slow-growing white oak seedlings appeared to be more efficient in utilizing light than northern red or chestnut oak seedlings. This suggests no additional benefits to increasing light above that typically found in a light shelterwood cut; however, it is crucial to control faster-growing competing vegetation. C1 [Rebbeck, Joanne; Scherzer, Amy] US Forest Serv, No Res Stn, USDA, Delaware, OH 43015 USA. [Gottschalk, Kurt] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. RP Rebbeck, J (reprint author), US Forest Serv, No Res Stn, USDA, 359 Main Rd, Delaware, OH 43015 USA. EM jrebbeck@fs.fed.us FU US Forest Service, Northern Research Station FX The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the US Department of Agriculture or the Forest Service of any product or service to the exclusion of others that may be suitable. The authors thank David Feicht, David Hosack, Arthur Peterson, Brian Simpson, and Mary Ann Tate for their highly valued technical assistance. The manuscript was improved appreciably by the comments of Lisa Samuelson, Susana Sung, and three anonymous reviewers. We thank John Stanovick for a biometrics review. This research was supported by the US Forest Service, Northern Research Station. NR 51 TC 7 Z9 10 U1 2 U2 18 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2012 VL 42 IS 6 BP 1025 EP 1037 DI 10.1139/X2012-057 PG 13 WC Forestry SC Forestry GA 957IQ UT WOS:000305155500004 ER PT J AU Gronewold, C D'Amato, AW Palik, BJ AF Gronewold, Chris D'Amato, Anthony W. Palik, Brian J. TI Relationships between growth, quality, and stocking within managed old-growth northern hardwoods SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID UNEVEN-AGED SILVICULTURE; STAND-DENSITY INDEX; DIAMETER DISTRIBUTION; DOUGLAS-FIR; FORESTS; EFFICIENCY; 2ND-GROWTH; PONDEROSA; LEVEL; TREES AB An understanding of long-term growth dynamics is central to the development of sustainable uneven-aged silvicultural systems for northern hardwood forests in eastern North America. Of particular importance are quantitative assessments of the relationships between stocking control and long-term growth and quality development. This study examined these relationships in a long-term silviculture experiment established in northern hardwood stands in the Upper Peninsula of Michigan, USA. Stands were old growth at the onset of the experiment and were maintained at three residual stocking levels (11.5, 16.1, and 20.7 m(2).ha(-1)) over a 57-year period. Several aspects of long-term stocking control were evaluated, including the effects of residual stocking on tree quality development and the relationships between stand stocking and individual tree growth and stand-level production. Results suggest that residual stocking had little impact on quality development, likely due to the initial old-growth condition of the stands examined. In contrast, our results indicate that a range of stand densities will maintain acceptable rates of stand-level production in selection systems and that growth can be shifted between diameter classes depending on desired future stand conditions. C1 [Gronewold, Chris; D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Palik, Brian J.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Gronewold, C (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM gron0108@umn.edu FU Minnesota Agricultural Experiment Station; USDA Forest Service Northern Research Station FX The authors wish to acknowledge the outstanding efforts of past USDA Forest Service scientists and technicians who established and maintained the Cutting Cycles and Stocking Levels Experiment, particularly Terry Strong, Gayne G. (Gus) Erdmann, Thomas Crow, and Christel Kern. Caleb Anderson assisted with data collection during the 2008 measurement season. Comments from anonymous reviewers greatly improved this manuscript. Funding for this work was provided by the Minnesota Agricultural Experiment Station and USDA Forest Service Northern Research Station. NR 48 TC 1 Z9 1 U1 1 U2 22 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2012 VL 42 IS 6 BP 1115 EP 1125 DI 10.1139/X2012-070 PG 11 WC Forestry SC Forestry GA 957IQ UT WOS:000305155500011 ER PT J AU Witmer, GW Snow, NP Moulton, RS Swartz, JL AF Witmer, Gary W. Snow, Nathan P. Moulton, Rachael S. Swartz, Jenna L. TI An assessment of seedling damage by wild house mice (Mus musculus) and wild deer mice (Peromyscus spp.) SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID ALTERNATIVE FOODS; PINE SEED; PREDATION; ESTABLISHMENT; POPULATIONS; RODENTS AB Although it is known that voles will damage seedlings, we do not know the extent to which deer mice (Peromyscus spp.) and house mice (Mus musculus Linnaeus, 1758) damage seedlings. Knowing this information can assist resource managers in better targeting problem species and implementing appropriate management actions. We planted and monitored ponderosa pine (Pinus ponderosa Douglas ex Lawson & C. Lawson) and narrow-leaf cottonwood (Populus angustifolia E. James) seedlings in metal stock tanks occupied by deer mice or house mice to assess the potential for damage by these rodents. Both species damaged leaves and stems of cottonwood seedlings, but house mice did more damage. House mouse damage resulted in mortality of over half of the cottonwood seedlings, whereas deer mice caused a much lower level of seedling mortality. Only slight damage was done by either species to pine seedlings. Neither species damaged the roots of seedlings, despite the extensive burrowing by house mice. Although voles are often considered to be the primary rodent species causing seedling damage, we have shown that deer mice and especially house mice could also cause substantial damage to deciduous seedlings. However, our work suggests that rodent control to prevent damage to conifer seedlings might not be warranted in general unless there are extenuating circumstances and the species causing the damage are identified to assist with targeting control methods more precisely. C1 [Witmer, Gary W.; Snow, Nathan P.; Moulton, Rachael S.; Swartz, Jenna L.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Witmer, GW (reprint author), Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM gary.w.witmer@aphis.usda.gov FU U.S. Department of Agriculture Wildlife Services FX This study was funded by the U.S. Department of Agriculture Wildlife Services. The study was conducted under the NWRC IACUC approved study protocol QA-1754. We thank the various landowners for allowing us to trap mice on their properties for use in this study. NR 22 TC 2 Z9 2 U1 0 U2 7 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2012 VL 42 IS 6 BP 1168 EP 1172 DI 10.1139/X2012-065 PG 5 WC Forestry SC Forestry GA 957IQ UT WOS:000305155500015 ER PT J AU Otrosina, WJ Zarnoch, SJ AF Otrosina, William J. Zarnoch, Stanley J. TI Response of different white fir geographic provenances to Trichosporium symbioticum inoculation in California SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article ID SCOLYTUS-VENTRALIS COLEOPTERA; ABIES-GRANDIS; ENGRAVER; BEETLE; ATTACK AB We inoculated the fir engraver (Scolytus ventralis LeConte) associated fungus Trichosporium symbioticum Wright onto 56 white fir (Abies concolor (Gordon & Glend.) Lindl. ex Hildebr.) trees planted in a common garden study near Camino, California, that represented five geographic provenances of this species. The objective was to determine if there is a differential lesion length response of white fir provenances with respect to provenance. We found a significant (P < 0.019) difference between the provenances from Arizona and those of eastern Nevada origins 28 days after inoculation. There was a significant interaction between the two T. symbioticum isolates and season of inoculation. Fall inoculations tended to have smaller lesions than those in the spring but this varied by isolate in that the one from eastern Nevada tended to produce longer lesions in the spring (P = 0.0001) whereas the isolate from the Camino plantation did not differ between spring and fall (P = 1.000). There is evidence for genetic variability relative to white fir provenance lesion length in response to T. symbioticum inoculation, and in future studies, isolate variability should also be taken into account. C1 [Otrosina, William J.; Zarnoch, Stanley J.] US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. RP Otrosina, WJ (reprint author), US Forest Serv, USDA, So Res Stn, Athens, GA 30602 USA. EM wotrosina@fs.fed.us NR 16 TC 0 Z9 0 U1 1 U2 3 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0045-5067 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD JUN PY 2012 VL 42 IS 6 BP 1178 EP 1183 DI 10.1139/X2012-059 PG 6 WC Forestry SC Forestry GA 957IQ UT WOS:000305155500017 ER PT J AU Shirk, PD Shapiro, JP Reitz, SR Thomas, JMG Koenig, RL Hay-Roe, MM Buss, LJ AF Shirk, Paul D. Shapiro, Jeffrey P. Reitz, Stuart R. Thomas, Jean M. G. Koenig, Rosalie L. Hay-Roe, Mirian M. Buss, Lyle J. TI Predator-Prey Relationships on Apiaceae at an Organic Farm SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE banker plants; Orius; minute pirate bugs; Frankliniella; thrips ID ORIUS-INSIDIOSUS HETEROPTERA; FLOWER THRIPS THYSANOPTERA; INTEGRATED PEST-MANAGEMENT; BIOLOGICAL-CONTROL; ANTHOCORIDAE; HEMIPTERA; PEPPER; FLORIDA AB Orius insidiosus (Say) and O. pumilio (Champion) were confirmed to be sympatric in north central Florida as the major predators of the Florida flower thrips, Frankliniella bispinosa (Morgan), on flowers of Queen Anne's lace, Daucus carota L. and false Queen Anne's lace, Ammi majus L. F. bispinosa was the predominant thrips observed on both flowers but colonized D. carota to a greater extent and earlier in the season than A. majus. Despite differences in the abundance of F. bispinosa on the two plants, neither Orius species showed host plant affinities. Population profiles for the thrips and Orius spp. followed a density dependent response of prey to predator with a large initial prey population followed by a rapid decline as the predator populations increased. The temporal increases in Orius spp. populations during the flowering season suggest that they were based on reproductive activity. As observed in a previous study, O. insidiosus had a larger population than O. pumilio and also had a predominantly male population on the flowers. By examining carcasses of the prey, there appeared to be no sexual preference of the thrips as prey by the Orius spp. as the prey pattern followed the demographics of the thrips sex ratio. Few immatures of either thrips or Orius spp. were observed on D. carota or A. majus, which suggests that oviposition and nymphal development occurred elsewhere. Based on these findings, D. carota and A. majus could serve as a banker plant system for Orius spp. C1 [Shirk, Paul D.; Shapiro, Jeffrey P.; Thomas, Jean M. G.; Hay-Roe, Mirian M.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. [Reitz, Stuart R.] ARS, Ctr Med Agr & Vet Entomol, USDA, Tallahassee, FL 32308 USA. [Koenig, Rosalie L.] Rosies Organ Farm, Gainesville, FL 32607 USA. [Koenig, Rosalie L.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Buss, Lyle J.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. RP Shirk, PD (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. EM paul.shirk@ars.usda.gov NR 25 TC 7 Z9 7 U1 0 U2 24 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 487 EP 496 DI 10.1603/EN11232 PG 10 WC Entomology SC Entomology GA 961OP UT WOS:000305475600010 PM 22732606 ER PT J AU Yokoyama, VY Wang, XG Aldana, A Caceres, CE Yokoyama-Hatch, HA Rendon, PA Johnson, MW Daane, KM AF Yokoyama, Victoria Y. Wang, Xin-Geng Aldana, Alicia Caceres, Carlos E. Yokoyama-Hatch, Hana A. Rendon, Pedro A. Johnson, Marshall W. Daane, Kent M. TI Performance Of Psyttalia Humilis (Hymenoptera: Braconidae) Reared From Irradiated Host on Olive Fruit Fly ( Diptera: Tephritidae) In California SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE larval parasitoid; Bactrocera oleae (Rossi); irradiated host; parasitism; longevity ID BIOLOGICAL-CONTROL; DIACHASMIMORPHA-LONGICAUDATA; CONCOLOR HYMENOPTERA; CERATITIS-CAPITATA; PARASITOIDS; RADIATION; LARVAE; POPULATIONS; PUPAE; SIZE AB The parasitoid Psyttalia humilis (Silvestri) was reared on Mediterranean fruit fly, Ceratitis capitata (Wiedemann), third instars irradiated at 0-70 Gy at the USDA, APHIS, PPQ, Moscamed biological control laboratory in San Miguel Petapa, Guatemala, and shipped to the USDA, ARS, Parlier, CA. Irradiation dose did not affect the parasitoid's offspring sex ratio (53-62% females), percentage of unemerged adults (12-34%), number of progeny produced per female (1.4-1.8), and parasitism (19-24%). Host irradiation dose had no significant effect on the forewing length of female P. humilis and its parasitism on olive fruit fly, Bactrocera oleae (Rossi) and offspring sex ratio, but dissection of 1-wk-old female parasitoids reared from hosts irradiated with 70 Gy had a significantly lower number of mature eggs than females from nonirradiated hosts. Longevity of P. humilis adults decreased with increased temperature from 15 to 35 degrees C, regardless of food provisions, gender, and host irradiation dose. Females survived 37-49 d at 15 degrees C with water and food, and only 1-2 d at 35 degrees C without food, whereas males lived shorter than females at all temperatures and food combinations tested. Adult P. humilis reared from fertile C. capitata and aspirated for dispensing in cups lived significantly longer after shipment than those specimens chilled and dispensed by weight. At 21 and 32 degrees C, 50% of parasitoids departed release cages after 180 and 30 min, respectively, but none departed at 12 degrees C. Thirteen shipments of P. humilis (2,980-21,922 parasitoids per shipment) were received between September and December 2009, and seven shipments (7,502-22,560 parasitoids per shipment) were received between October and December 2010 from San Miguel Petapa, Guatemala. Daily number of olive fruit fly adult and percentage female trap captures ranged <1-19 and 8-58% in 2009, and <1-11 and 0-42% in 2010, respectively. The number of parasitoids released ranged 848-12,257 in 2009 and 3,675-11,154 in 2010. Percentage parasitism of olive fruit fly third instars at all locations ranged 0-9% in 2009 and 0-36% in 2010. C1 [Yokoyama, Victoria Y.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Wang, Xin-Geng; Daane, Kent M.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Aldana, Alicia; Caceres, Carlos E.; Rendon, Pedro A.] USDA APHIS PPQ CPHST, Guatemala City 10, Guatemala. [Yokoyama-Hatch, Hana A.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Johnson, Marshall W.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Yokoyama, VY (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM victoria.yokoyama@ars.usda.gov FU California Olive Committee, Fresno, CA; USDA CSREES Special Grants Program; Pest Management Alternatives; Moscamed Program, USA-Mexico-Guatemala FX We are grateful to Julie A. Gagnon, Sean K. Pelham, and Tim De Silva, USDA-ARS, San Joaquin Valley Agricultural Sciences Center, Parlier, CA, for assistance with this project, and to the numerous cooperators who have allowed us to use their olive groves. This research was funded in part by the California Olive Committee, Fresno, CA; the USDA CSREES Special Grants Program: Pest Management Alternatives; and the Moscamed Program, USA-Mexico-Guatemala. NR 40 TC 8 Z9 8 U1 1 U2 6 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 497 EP 507 DI 10.1603/EN11252 PG 11 WC Entomology SC Entomology GA 961OP UT WOS:000305475600011 PM 22732607 ER PT J AU Light, DM Beck, JJ AF Light, Douglas M. Beck, John J. TI Behavior of Codling Moth (Lepidoptera: Tortricidae) Neonate Larvae on Surfaces Treated With Microencapsulated Pear Ester SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE larvae; arrestment; kairomone; pear ester; microencapsulated ID CYDIA-POMONELLA; LASPEYRESIA-POMONELLA; ALPHA-FARNESENE; POME FRUIT; OLETHREUTIDAE; APPLE; ATTRACTION; KAIROMONE; GRANULOVIRUS; OVIPOSITION AB Codling moth, Cydia pomonella (L.), larvae cause severe internal feeding damage to apples, pears, and walnuts worldwide. Research has demonstrated that codling moth neonate first instar larvae are attracted to a pear-derived kairomone, ethyl (2E, 4Z)-2,4-decadienoate, the pear ester (PE). Reported here are the behavioral activities of neonate codling moth larvae to microencapsulated pear ester(MEC-PE) applied in aqueous solutions to both filter paper and apple leaf surfaces that were evaluated over a period of up to 20 d of aging. In dual-choice tests the MEC-PE treatment elicited attraction to and longer time spent on treated zones of filter papers relative to water-treated control zones for up to 14 d of aging. A higher concentration of MEC-PE caused no preferential response to the treated zone for the first 5 d of aging followed by significant responses through day 20 of aging, suggesting sensory adaptation as an initial concentration factor. Estimated emission levels of PE from treated filter papers were experimentally calculated for the observed behavioral thresholds evident over the aging period. When applied to apple leaves, MEC-PE changed neonate walking behavior by eliciting more frequent and longer time periods of arrestment and affected their ability to find the leaf base and stem or petiole. Effects of MEC-PE on extended walking time and arrestment by codling moth larvae would increase temporal and spatial exposure of neonates while on leaves; thereby potentially disrupting fruit or nut finding and enhancing mortality by increasing the exposure to insecticides, predation, and abiotic factors. C1 [Light, Douglas M.; Beck, John J.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Light, DM (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM doug.light@ars.usda.gov FU USDA ARS CRIS project [5325-42000-037-00] FX The authors thank James Baker for his technical assistance and Trece, Inc., for their cooperation and samples of microencapsulated pear ester. This research was conducted under USDA ARS CRIS project 5325-42000-037-00. This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or recommendation by the USDA for its use. NR 39 TC 5 Z9 5 U1 2 U2 23 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 603 EP 611 DI 10.1603/EN11273 PG 9 WC Entomology SC Entomology GA 961OP UT WOS:000305475600023 PM 22732619 ER PT J AU Woods, JL Dreves, AJ Fisher, GC James, DG Wright, LC Gent, DH AF Woods, J. L. Dreves, A. J. Fisher, G. C. James, D. G. Wright, L. C. Gent, D. H. TI Population Density and Phenology of Tetranychus urticae (Acari: Tetranychidae) in Hop is Linked to the Timing of Sulfur Applications SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE conservation biological control; Humulus lupulus; pest resurgence; Podosphaera macularis ID 2-SPOTTED SPIDER-MITE; NATURAL ENEMIES; NEOSEIULUS-FALLACIS; PHYTOPHAGOUS MITES; BIOLOGICAL-CONTROL; PHYTOSEIIDAE; ECOLOGY; FUNGICIDE; IMPACT; PREDATORS AB The twospotted spider mite, Tetranychus urticae Koch, is a worldwide pest of numerous agronomic and horticultural plants. Sulfur fungicides are known to induce outbreaks of this pest on several crops, although mechanisms associated with sulfur-induced mite outbreaks are largely unknown. Studies were conducted during 2007 2009 in Oregon and Washington hop yards to evaluate the effect of timing of sulfur applications on T. urticae and key predators. In both regions, applications of sulfur made relatively late in the growing season (mid-June to mid-July) were associated with the greatest exacerbation of spider mite outbreaks, particularly in the upper canopy of the crop. The severity of mite outbreaks was closely associated with sulfur applications made during a relatively narrow time period coincident with the early exponential phase of spider mite increase and rapid host growth. A nonlinear model relating mean cumulative mite days during the time of sulfur sprays to the percent increase in total cumulative mite days (standardized to a nontreated plot) explained 58% of the variability observed in increased spider mite severity related to sulfur spray timing. Spatial patterns of spider mites in the Oregon plots indicated similar dispersal of motile stages of spider mites among leaves treated with sulfur versus nontreated leaves; however, in two of three years, eggs were less aggregated on leaves of sulfur-treated plants, pointing to enhanced dispersal. Apart from one experiment in Washington, relatively few predatory mites were observed during the course of these studies, and sulfur-induced mite outbreaks generally occurred irrespective of predatory mite abundance. Collectively, these studies indicate sulfur induces mite outbreaks through direct or indirect effects on T. urticae, mostly independent of predatory mite abundance or toxicity to these predators. Avoidance of exacerbation of spider mite outbreaks by sulfur sprays was achieved by carefully timing applications to periods of low spider mite abundance and slower host development, which is generally early to mid-spring for hop. C1 [Gent, D. H.] ARS, USDA, Forage & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Woods, J. L.; Dreves, A. J.; Fisher, G. C.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [James, D. G.; Wright, L. C.] Washington State Univ, Irrigated Agr Res & Extens Ctr, Dept Entomol, Prosser, WA 99350 USA. RP Gent, DH (reprint author), ARS, USDA, Forage & Cereal Res Unit, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU Hop Research Council; Washington Hop Commission; Oregon State University; Washington State Commission on Pesticide Registration; Washington State University; USDA-ARS CRIS Project [5358-21000-050-00]; USDA-CSREES Western Integrated Pest Management (IPM) Center Award [2007-34103-18579] FX The authors gratefully acknowledge technical support from Nanci Adair, Chase Annen, Deborah Brooks, Oscar Garcia, Suzy Kropf, Kazandra Lewis, Leah Mancino, Katie Roseboro, Marina Mireles, and Victor Reyna. Appreciation is extended to Gerry Krantz and Frederic Beaulieu for assistance with predatory mite identification. We also thank Elizabeth Beers and Jana Lee for their review of an earlier draft of the manuscript. Funding for these studies was provided from the Hop Research Council, Washington Hop Commission, Oregon State University, Washington Hop Commission, Washington State Commission on Pesticide Registration, Washington State University, USDA-ARS CRIS Project 5358-21000-050-00, and USDA-CSREES Western Integrated Pest Management (IPM) Center Award 2007-34103-18579. NR 55 TC 5 Z9 6 U1 2 U2 25 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 621 EP 635 DI 10.1603/EN11279 PG 15 WC Entomology SC Entomology GA 961OP UT WOS:000305475600025 PM 22732621 ER PT J AU Ranger, CM Reding, ME Schultz, PB Oliver, JB AF Ranger, Christopher M. Reding, Michael E. Schultz, Peter B. Oliver, Jason B. TI Ambrosia Beetle (Coleoptera: Curculionidae) Responses to Volatile Emissions Associated With Ethanol-Injected Magnolia virginiana SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE ambrosia beetles; Xylosandrus germanus; ethanol; solid phase microextraction ID SOLID-PHASE MICROEXTRACTION; XYLOSANDRUS-GERMANUS; BAITED TRAPS; DOUGLAS-FIR; ORNAMENTAL NURSERIES; BARK BEETLES; SCOLYTINAE; STRESS; PLANTS; TREES AB Xylosandrus germanus (Blandford) and other species of ambrosia beetles are key pests of ornamental nursery trees. A variety of laboratory- and field-based experiments were conducted in pursuit of improved monitoring strategies and to develop a trap tree strategy for ambrosia beetles. Traps baited with bolts prepared from Magnolia virginiana L. injected with ethanol caught five times more X. germanus than ethanol-baited traps. Basal stem injections of ethanol into M. virginiana induced more ambrosia beetle attacks than irrigating or baiting with ethanol, and no attacks occurred on water-injected trees. A positive correlation was also detected between concentration of injected ethanol and cumulative attacks. Solid phase microextraction-gas chromatography-mass spectrometry characterized bark emissions from ethanol- and water-injected M. virginiana at 1, 2, 10, and 16 d after treatment. Ethanol emission from injected trees steadily declined from 1 to 16 d after treatment, but was not emitted from water-injected trees. A variety of monoterpenes were also emitted in trace amounts from the ethanol- and water-injected trees. Antennal responses of X. germanus via gas chromatography-electroantennographic detection to volatiles from ethanol- injected M. virginiana occurred for ethanol, but not the various monoterpenes. X. germanus and other ambrosia beetles were also equally attracted to traps baited with ethanol alone compared with a synthetic mixture of ethanol plus various monoterpenes formulated to mimic ethanol-injected M. virginiana. Injecting concentrated solutions of ethanol into trees may be useful for establishing odor-based trap trees, which could aid with monitoring programs and/or potentially deflect ambrosia beetles away from valuable nursery stock. C1 [Ranger, Christopher M.; Reding, Michael E.] ARS, USDA, Applicat Technol Res Unit, Hort Insects Res Lab, Wooster, OH 44691 USA. [Ranger, Christopher M.; Reding, Michael E.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Schultz, Peter B.] Virginia Tech, Hampton Rd Agr Res & Extens Ctr, Virginia Beach, VA 23455 USA. [Oliver, Jason B.] Tennessee State Univ, Coll Agr Human & Nat Sci, Otis L Floyd Nursery Res Ctr, Mcminnville, TN 37110 USA. RP Ranger, CM (reprint author), ARS, USDA, Applicat Technol Res Unit, Hort Insects Res Lab, 1680 Madison Ave, Wooster, OH 44691 USA. EM christopher.ranger@ars.usda.gov FU USDA-ARS Floriculture and Nursery Research Initiative; USDA-APHIS; ARS [3607-22000-012-00D] FX We thank James Moyseenko, Jennifer Barnett, Gerald Hammel, Leslie Morris, and Betsy Anderson for technical assistance. The lead author also thanks Brian Sullivan (USDA-FS) for training in ambrosia beetle antennal preparations for electrophysiological purposes. This research was supported by funding from the USDA-ARS Floriculture and Nursery Research Initiative, USDA-APHIS' Request for Technology Development to Improve Detection and Survey for Exotic Wood Borers, and base funds associated with ARS Research Project 3607-22000-012-00D (National Program 304-Crop Protection and Quarantine). NR 42 TC 16 Z9 17 U1 0 U2 20 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 636 EP 647 DI 10.1603/EN11299 PG 12 WC Entomology SC Entomology GA 961OP UT WOS:000305475600026 PM 22732622 ER PT J AU Ryall, KL Silk, PJ Mayo, P Crook, D Khrimian, A Cosse, AA Sweeney, J Scarr, T AF Ryall, Krista L. Silk, Peter J. Mayo, Peter Crook, Damon Khrimian, Ashot Cosse, Allard A. Sweeney, Jon Scarr, Taylor TI Attraction of Agrilus planipennis (Coleoptera: Buprestidae) to a Volatile Pheromone: Effects of Release Rate, Host Volatile, and Trap Placement SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE emerald ash borer; pheromone; dose-response; (3Z)-dodecen-12-olide; lactone ID EMERALD ASH BORER; TETROPIUM-CINNAMOPTERUM KIRBY; GREEN LEAF VOLATILES; AGGREGATION PHEROMONE; PLANT VOLATILES; MELOLONTHA-HIPPOCASTANI; PRISM TRAPS; LOW-DENSITY; FUSCUM F; FAIRMAIRE AB Attraction of emerald ash borer, Agrilus planipennis Fairmaire, to a volatile pheromone was demonstrated in three field experiments using baited green sticky traps. A dose-Dresponse curve was generated for male A. planipennis to increasing release rates of (3Z)-dodecen-12-olide ((3Z)-lactone) in combination with the green leaf volatile, (3Z)-hexenol. Only the lowest release rate (<2.50 mu g/ d) of (3Z)-lactone significantly increased captures of male A. planipennis, as compared with traps baited with (3Z)-hexenol alone. Effect of trap height, (3Z)-lactone, and (3Z)-hexenol and their interactions on the trap capture of A. planipennis was determined in a factorial experiment. Number of males per trap was significantly and positively affected by (3Z)-lactone, (3Z)-hexenol, and trap height whereas number of females per trap was affected by trap height only; none of the interactions were significant. As predicted, the greatest mean catch of males was in traps baited with (3Z)-lactone and (3Z)-hexenol placed high in the canopy. Electroantennogram tests on the bark volatile, 7-epi-sesquithujene, demonstrated the ability of male and female A. planipennis antennae to detect and respond to this compound, particularly the (+)-7-epi-sesquithujene isomer. Results from an olfactometer bioassay and field testing did not demonstrate attraction of either males or females to (+)-7-epi-sesquithujene. These data increase our understanding of the pheromone ecology of the invasive emerald ash borer, provide further confirmation of the behavioral activity of the female-produced lactone pheromone, and should increase the ability to detect A. planipennis infestations where they are present. C1 [Ryall, Krista L.] Nat Resources Canada, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2G3, Canada. [Silk, Peter J.; Mayo, Peter; Sweeney, Jon] Nat Resources Canada, Atlantic Forestry Ctr, Fredericton, NB E3B 5P7, Canada. [Crook, Damon] USDA, Anim & Plant Hlth Inspect Serv, PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Khrimian, Ashot] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Cosse, Allard A.] ARS, USDA, NCAUR, Peoria, IL 61604 USA. [Scarr, Taylor] Ontario Minist Nat Resources, Sault Ste Marie, ON P6A 6V5, Canada. RP Ryall, KL (reprint author), Nat Resources Canada, Great Lakes Forestry Ctr, 1219 Queen St E, Sault Ste Marie, ON P6A 2G3, Canada. EM kryall@nrcan.gc.ca FU Spray Efficacy Research Group International (SERG-I specifically, Ontario, Manitoba, and Saskatchewan Natural Resources Departments); Canadian Forest Service FX We thank the two anonymous reviewers for helpful comments that improved this manuscript. Wethank Hugh Evans, Isabelle Ochoa, Dave Nisbett, Kalle Wainio, Sarah Crispell, Luc Breton, and David Dutkiewicz for technical assistance in Canada. We thank the Spray Efficacy Research Group International (SERG-I specifically, Ontario, Manitoba, and Saskatchewan Natural Resources Departments) and the Canadian Forest Service for funding. The Canadian Food Inspection Agency also provided support for the research. In addition, USDA, APHIS PPQ CPHST provided traps and lures in support of these experiments. All experiments reported here comply with the laws of Canada. NR 53 TC 26 Z9 27 U1 2 U2 33 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 648 EP 656 DI 10.1603/EN11312 PG 9 WC Entomology SC Entomology GA 961OP UT WOS:000305475600027 PM 22732623 ER PT J AU Milbrath, LR Biazzo, J AF Milbrath, Lindsey R. Biazzo, Jeromy TI Development and Reproduction of the Foxglove Aphid (Hemiptera: Aphididae) on Invasive Swallow-Worts (Vincetoxicum spp.) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE foxglove aphid; Aulacorthum solani; swallow-wort; Vincetoxicum; host suitability ID AULACORTHUM-SOLANI HEMIPTERA; LIFE TABLE; TEMPERATURES; ROSSICUM; POPULATIONS; HOMOPTERA; PLANTS; VINE AB The foxglove aphid, Aulacorthum solani (Kaltenbach), recently was documented using the invasive species pale and black swallow-wort (Vincetoxicum rossicum (Kleopow) Barbar. and V. nigrum (L.) Moench, respectively) as host plants. Because these are new host plant records for this polyphagous species, we investigated foxglove aphid development and reproduction on pale and black swallow-wort relative to a known crop host, potato (Solanum tuberosum L.), at a 25:20 degrees C thermophase and a photoperiod of 16:8 (L:D) h. Almost no such data have been previously reported for a noneconomic host plant. Larval development and survival, as well as adult reproductive development and fecundity, were similar between the two swallow-wort species and potato. Adult aphids lived significantly longer on pale swallow-wort than the other two host plants, but this extended longevity encompassed the postreproductive stage. Foxglove aphid population parameters were therefore similar among the three plant species as well as most previous reports on crop plants. Pale and black swallow-wort appear to be suitable secondary hosts for foxglove aphid; other factors possibly limit aphid abundance on these two plant species in the field. C1 [Milbrath, Lindsey R.; Biazzo, Jeromy] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Milbrath, LR (reprint author), ARS, USDA, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM lindsey.milbrath@ars.usda.gov NR 24 TC 1 Z9 1 U1 0 U2 14 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 665 EP 668 DI 10.1603/EN11239 PG 4 WC Entomology SC Entomology GA 961OP UT WOS:000305475600029 PM 22732625 ER PT J AU Simmons, AM Legaspi, JC Legaspi, BC AF Simmons, Alvin M. Legaspi, Jesusa C. Legaspi, Benjamin C., Jr. TI Adult Survival of Delphastus catalinae (Coleoptera: Coccinellidae), a Predator of Whiteflies (Hemiptera: Aleyrodidae), on Diets of Whiteflies, Honeydew, and Honey SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Bemisia tabaci; Delphastus catalinae; biological control; predator; diet ID BEMISIA-ARGENTIFOLII HOMOPTERA; BIOLOGICAL-CONTROL; IMMATURE SURVIVAL; TABACI; HYMENOPTERA; OVIPOSITION; APHELINIDAE; AUSTRALIA; LONGEVITY; HALDEMAN AB Delphastus catalinae (Horn) is a coccinellid predator that is commercially sold for the management of whiteflies. A study was conducted to assay the effect of selected diets on the survival of adult D. catalinae. Treatments of water (as a control), 10% honey, honeydew, and whiteflies [Bemisia tabaci (Gennadius)] were provided to the beetles in laboratory assays. Newly emerged, unfed adult insects were used at the start of a survival experiment with trials lasting 50 d. Another survival experiment used mixed-aged adults from a greenhouse colony, and the trials lasted 21 d. Survival was poor on a diet of solely water; approximate to 1% survived beyond a week at 26 degrees C. Survival using the newly emerged insects was similar between those fed honeydew and honey diets, but those on the whitefly diet had the greatest survival (approximate to 60% on day 50). However, in the experiment with mixed-aged beetles, adults on honey, and whitefly diets performed the same over a 21-d experiment. Excluding those on the water diet, survival of beetles on the various diets ranged from approximate to 50-80% after 21 d. In an open choice assay across 7 h, D. catalinae adults were found on the whitefly diet in a much greater incidence than on the other diets, and the number of beetles found on the whitefly diet increased over time. The data supports that when D. catalinae are employed in greenhouses or fields for whitefly management, during low prey populations, honeydew from the whitefly can help sustain the population of this predator. Moreover, a supplemental food such as a honey solution can help sustain the population of D. catalinae when the prey is decreased to low numbers. These results may help in the development of strategies to enhance the utility of predators for the management of whiteflies. C1 [Simmons, Alvin M.; Legaspi, Jesusa C.; Legaspi, Benjamin C., Jr.] USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Simmons, AM (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM alvin.simmons@ars.usda.gov NR 42 TC 2 Z9 2 U1 1 U2 28 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 669 EP 675 DI 10.1603/EN11247 PG 7 WC Entomology SC Entomology GA 961OP UT WOS:000305475600030 PM 22732626 ER PT J AU Chen, HL Zhang, HY Zhu, KY Throne, JE AF Chen, Haoliang Zhang, Hongyu Zhu, Kun Yan Throne, James E. TI Induction of Reproductive Diapause in Habrobracon hebetor (Hymenoptera: Braconidae) When Reared at Different Photoperiods at Low Temperatures SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE biological control; ectoparasitoid; Habrobracon hebetor; photoperiod; reproductive diapause ID ALMOND MOTH LEPIDOPTERA; INSECT FAT-BODY; PARASITIC HYMENOPTERA; INDIANMEAL MOTH; LARVAL DIAPAUSE; ADULT DIAPAUSE; COLD-STORAGE; HETEROPTERA; PYRALIDAE; WASP AB Development of the parasitoid Habrobracon hebetor (Say) (Hymenoptera: Braconidae) at low temperatures was determined to identify rearing conditions that might result in adults that were in reproductive diapause. Diapausing adults would be expected to survive cold storage longer than nondiapausing adults for use in biological control programs. Only a few eggs were found in the ovaries when H. hebetor females were reared during the immature stages at 17.5 and 20 degrees C with a 16-h photoperiod, and the ovaries were poorly developed and contained no eggs when females were reared with a 10-h photoperiod in these low temperatures. Rearing H. hebetor at 17.5 and 20 degrees C did not result in diapause of immature stages, but did appear to result in possible adult reproductive diapause when the immature stages were reared with a 10-h photoperiod. Females reared during the immature stages at 17.5 degrees C with a10-h photoperiod lived longer and took longer to lay their first eggs and to lay 50% of their eggs than those females reared at 17.5 degrees C with a16-h photoperiod. Females reared during the immature stages at 20 degrees C with a10-h photoperiod took longer to lay their first eggs and to lay 50% of their eggs, and they had a lower respiration rate, than those females reared at 20 degrees C with a16-h photoperiod. Females that were reared in conditions that appeared to induce reproductive diapause resumed oviposition and their respiration rate increased soon after being transferred to a higher temperature (27.5 degrees C). Thus, females reared at a 10-h photoperiod at 17.5 and 20 degrees C appear to enter reproductive diapause. C1 [Chen, Haoliang; Zhang, Hongyu] Huazhong Agr Univ, State Key Lab Agr Microbiol, Inst Urban & Hort Pests, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China. [Chen, Haoliang; Zhang, Hongyu] Huazhong Agr Univ, Hubei Key Lab Insect Resource Applicat & Sustaina, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China. [Chen, Haoliang] Anhui Acad Agr Sci, Inst Plant Protect, Hefei 230031, Anhui, Peoples R China. [Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Throne, James E.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Zhang, HY (reprint author), Huazhong Agr Univ, State Key Lab Agr Microbiol, Inst Urban & Hort Pests, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China. EM hongyu.zhang@mail.hzau.edu.cn FU China National Science and Technology [2006BAI09B04-06, 2006BAD02A18-03]; Earmarked Fund for Modern Agro-industry Technology Research System; China Scholarship Council FX We thank Judy Johnson (USDA-ARS, Parlier, CA) for providing the Habrobracon hebetor used in the study and for reviewing an earlier version of the manuscript, and Joel Perez-Mendoza and Ann Redmon for their technical assistance. This research was supported by the China National Science and Technology Project of the 11th Five-Year Plan (2006BAI09B04-06 and 2006BAD02A18-03), the Earmarked Fund for Modern Agro-industry Technology Research System to H. Zhang, and a scholarship from the China Scholarship Council to H. Chen. Contribution no. 12-183-J from the Kansas Agricultural Experiment Station. NR 60 TC 4 Z9 6 U1 0 U2 17 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0046-225X J9 ENVIRON ENTOMOL JI Environ. Entomol. PD JUN PY 2012 VL 41 IS 3 BP 697 EP 705 DI 10.1603/EN11311 PG 9 WC Entomology SC Entomology GA 961OP UT WOS:000305475600033 PM 22732629 ER PT J AU White, WH Erwin, TL Viator, BJ AF White, W. H. Erwin, T. L. Viator, B. J. TI LEPTOTRACHELUS DORSALIS (COLEOPTERA: CARABIDAE): A CANDIDATE BIOLOGICAL CONTROL AGENT OF THE SUGARCANE BORER IN LOUISIANA SO FLORIDA ENTOMOLOGIST LA English DT Article DE Diatraea saccharalis; fire ants; habitat manipulation; augmentative release; predators ID LEPIDOPTERA; PYRALIDAE; SUSCEPTIBILITY; TEBUFENOZIDE; CRAMBIDAE; PREDATORS; FIELDS AB Following registration and the wide-spread use of insect growth regulators (e.g. tebufenozide and novaluron) for control of sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), in Louisiana, larvae of the ground beetle, Leptotrachelus dorsalis (F.) (Coleoptera: Carabidae, have become more abundant in sugarcane fields. In a survey of the 18 parishes growing sugarcane in Louisiana, L. dorsalis was found in 8 (44%) of those parishes. The highest number of beetles occurred in a field where 10% of the stalks sampled were harboring larvae. Laboratory studies revealed a developmental period of 37 d from egg deposition to adult emergence. Three larval instars were identified with the first 2 lasting 5 d, and the third instar requiring 17 d. In a voracity study, larvae were found to consume on average 798 first instar sugarcane borer larvae per ground beetle larva. Seventy-five percent of those sugarcane borer larvae were consumed by third instar beetle larvae. Field surveys suggest that adults migrate into sugarcane fields when above-ground internodes form on the sugarcane stalk (June) and increase in numbers thereafter. All 3 instars of the beetle can be found in sugarcane fields during the critical period of Jun to Sep when sugarcane is subject to economic injury by sugarcane borer. If L. dorsalis are abundant in fields during establishment of second generation sugarcane borer, our data suggests they are capable of holding the average season-long damage at or below 10% bored internodes. This level of damage is the recognized economic injury level for sugarcane borer in Louisiana. Our study indicates that L. dorsalis is a good candidate for continued research on augmentative releases as a strategy to increase beetle numbers in sugarcane fields early in the growing season and thus enhance their efficacy as predators of sugarcane borer larvae. C1 [White, W. H.] ARS, USDA, Sugarcane Res Unit, Houma, LA 70360 USA. [Erwin, T. L.] Smithsonian Inst, Natl Museum Nat Hist, Dept Entomol, Hyper Divers Grp, Washington, DC 20013 USA. [Viator, B. J.] Calvin Viator PhD & Associates LLC, Labadieville, LA 70372 USA. RP White, WH (reprint author), ARS, USDA, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. FU American Sugar Cane League of the U.S.A., Inc. FX The authors express gratitude to Randy Richard and Elta Duet for valuable assistance in the laboratory and field. Financial assistance from the American Sugar Cane League of the U.S.A., Inc. is gratefully acknowledged. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 21 TC 2 Z9 2 U1 0 U2 2 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 261 EP 267 PG 7 WC Entomology SC Entomology GA 960EL UT WOS:000305370700003 ER PT J AU Xue, RD Ali, A Barnard, DR AF Xue, Rui-De Ali, Arshad Barnard, Donald R. TI MATING STATUS AND BODY SIZE IN AEDES ALBOPICTUS (DIPTERA: CULICIDAE) AFFECT HOST FINDING AND DEET REPELLENCY SO FLORIDA ENTOMOLOGIST LA English DT Article DE mosquito; host seeking; landing; probing; repellent; Complete Protection Time ID CARBOHYDRATE AVAILABILITY; AEGYPTI; MOSQUITOS; AGE; INSEMINATION; ATTRACTANTS; SURVIVAL; BEHAVIOR; FEMALES; SEEKING AB Variations in the conditions accompanying mosquito development and mating can result in females of variable size that have not been inseminated. In this study, we compared the host finding activity of mated and unmated large and small Aedes albopictus (Skuse) and the repellency to these mosquitoes of 25% DEET. The percentage of females seeking the source of human odor in an olfactometer was significantly influenced by body size (P = 0.007) but not mating status (P = 0.07). Most respondents were large (71%) but 40% of all host seeking females were not inseminated. Landing rates by Ae. albopictus on human skin were influenced in screened cage tests by mating status (P < 0.0001) and body size (P = 0.004). Mated females exhibited the highest landing rates (17.3% [large] and 12.7% [small]) followed by unmated large females (7.3%) and unmated small females (6.4%). The Complete Protection Time (CPT) from mosquito landing provided by 25% DEET was significantly influenced by mating status (P = 0.002) and body size (P = 0.025). Unmated small females were repelled longer (7.5 h) than unmated large females (7.0 h) and longer than small and large mated females (6.3 h and 5.6 h, respectively). CPT using 25% DEET was inversely related to mosquito landing rate in all treatment groups with 75 min more protection on average from bites by unmated females compared with mated females. C1 [Xue, Rui-De] Anastasia Mosquito Control Dist, St Augustine, FL 32085 USA. [Ali, Arshad] Univ Florida, IFAS, Midflorida Res & Educ Ctr, Apopka, FL 32703 USA. [Barnard, Donald R.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Xue, RD (reprint author), Anastasia Mosquito Control Dist, 500 Old Beach Rd, St Augustine, FL 32085 USA. RI Ali, Arshad/J-6647-2015 FU [445-96]; [A057] FX V. Droit, and K. Posey provided technical assistance. This research was conducted according to protocol #445-96 approved by University of Florida, Institutional Review Board-01 (human subjects); volunteers gave informed consent prior to participation in the study. Animal care and use was in accordance with protocol # A057 approved by the University of Florida, Institutional Animal Care and Use Committee. NR 23 TC 0 Z9 0 U1 1 U2 9 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 268 EP 272 PG 5 WC Entomology SC Entomology GA 960EL UT WOS:000305370700004 ER PT J AU Francis, AW Kairo, MTK Roda, AL AF Francis, Antonio W. Kairo, Moses T. K. Roda, Amy L. TI DEVELOPMENTAL AND REPRODUCTIVE BIOLOGY OF PLANOCOCCUS MINOR (HEMIPTERA: PSEUDOCOCCIDAE) UNDER CONSTANT TEMPERATURES SO FLORIDA ENTOMOLOGIST LA English DT Article DE passionvine mealybug; development; survivorship; reproduction; life table parameters ID LIFE-HISTORY; MEALYBUG; HOMOPTERA AB Effect of temperature on the developmental and reproductive biology of the passionvine mealybug, Planococcus minor (Maskell) (Hemiptera: Pseudococcidae), was investigated on sprouted potatoes. P. minor was able to develop and complete its life cycle at 20, 25, and 29 degrees C. No eggs eclosed at 15 and 35 degrees C. The developmental time from egg to adult female was approximately 49 d at 20 degrees C, 31 d at 25 degrees C, and 27 d at 29 degrees C. Between 20 and 29 degrees C, 58-71% of eggs survived to adulthood. Female mealybugs made up 60-73% of the adult populations in the 3 temperature treatments. Preoviposition and oviposition times decreased as the temperature increased. Females reproduced sexually and produced the highest number of eggs (270 eggs/female) at 20 degrees C. Adult female longevity declined from 34 d at 20 degrees C to 19-22 d at the 2 higher temperatures. Adult males were short-lived and their longevity declined with increasing temperature. At 25 degrees C, the gross reproductive rate (GRR) and net reproductive rate (R-o) were estimated at 445.7 female/female and 325.4 female/female, respectively, the generation time (T-G) was 39.5 d, the intrinsic rate of increase (r(m)) was 0.147 (female/female/d), the finite rate of increase (lambda) was 1.158 (female/female/d), and the doubling time (DT) was 4.7 d. The ability of P. minor to develop, survive, and reproduce successfully from 20 to 29 degrees C suggests that the mealybug has the potential to develop and establish in climatic zones that fall within this temperature range. C1 [Francis, Antonio W.; Kairo, Moses T. K.] Florida A&M Univ, Coll Agr & Food Sci, Ctr Biol Control, Tallahassee, FL 32307 USA. [Roda, Amy L.] USDA APHIS PPQ Ctr Plant Hlth Sci & Technol, Subtrop Hort Res Stn, Miami, FL 33158 USA. RP Francis, AW (reprint author), Florida A&M Univ, Coll Agr & Food Sci, Ctr Biol Control, Tallahassee, FL 32307 USA. EM antoniowfrancis@gmail.com FU USDA APHIS PPQ; Florida AM University; Ministry of Agriculture, Land and Marine Resources, Trinidad and Tobago FX This study was funded under a cooperative agreement between USDA APHIS PPQ and Florida A&M University. We thank Elizabeth Johnson, Perry Polar, Anne DeGazon and other staff members at CABI Caribbean and Latin America, Curepe, Trinidad for assistance in providing research facilities and facilitating insectary setup and other essential services. We also acknowledge the support provided by the Ministry of Agriculture, Land and Marine Resources, Trinidad and Tobago. We thank Oscar Liburd, Lance Osborne, and Tim Momol, University of Florida and Raymond Hix, Florida A&M University for their comments and contributions to this study. We also extend our gratitude to Gilbert Queeley, Florida A&M University Cooperative Extension Services, Stuart Reitz, USDA ARS CMAVE, Tallahassee, Florida and Juang-Horng Chong, Clemson University for their help with the statistical analyses. NR 20 TC 0 Z9 3 U1 0 U2 16 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 297 EP 303 PG 7 WC Entomology SC Entomology GA 960EL UT WOS:000305370700009 ER PT J AU Landolt, PJ Adams, T Davis, TS Rogg, H AF Landolt, Peter J. Adams, Todd Davis, Thomas Seth Rogg, Helmuth TI SPOTTED WING DROSOPHILA, DROSOPHILA SUZUKII (DIPTERA: DROSOPHILIDAE), TRAPPED WITH COMBINATIONS OF WINES AND VINEGARS SO FLORIDA ENTOMOLOGIST LA English DT Article DE attractants; detection; lures; monitoring; spotted wing drosophila; traps ID UNITED-STATES; IDENTIFICATION; MELANOGASTER; LEPIDOPTERA; ATTRACTION; NOCTUIDAE; FRUITS; CHERRY AB Field trapping experiments evaluated wine and vinegar baits for spotted wing drosophila flies, Drosophila suzukii (Matsumura), and assessed variance in bait attractiveness with wine type, vinegar type, and bait age. A mixture of apple cider vinegar and a Merlot wine attracted more flies than a mixture of acetic acid and ethanol. The vinegar/wine mixture attracted numbers of flies that were similar to numbers of flies trapped with acetic acid with wine or ethanol with vinegar. These results indicate that chemicals in vinegar in addition to acetic acid, and chemicals in wine in addition to ethanol, are attractants for the spotted wing drosophila. Numbers of flies captured with wine/vinegar mixtures varied somewhat with wine type, with a Merlot wine yielding best captures among the wines tested. Numbers of flies captured with wine/vinegar mixes also varied somewhat with vinegar type, with a rice vinegar yielding best captures among vinegars tested. Numbers of flies captured varied little with bait age, from 0 to 7 days old. These results will assist efforts to improve baits used to trap spotted wing drosophila, and to provide guidance for the isolation and identification of chemical attractants from wines and vinegars. C1 [Landolt, Peter J.; Davis, Thomas Seth] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Adams, Todd; Rogg, Helmuth] Oregon Dept Agr, Salem, OR 97301 USA. RP Landolt, PJ (reprint author), ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov RI Marion-Poll, Frederic/D-8882-2011 OI Marion-Poll, Frederic/0000-0001-6824-0180 NR 34 TC 22 Z9 23 U1 1 U2 86 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 326 EP 332 PG 7 WC Entomology SC Entomology GA 960EL UT WOS:000305370700013 ER PT J AU Rohrig, EA Hall, DG Qureshi, JA Stansly, PA AF Rohrig, Eric A. Hall, David G. Qureshi, Jawwad A. Stansly, Philip A. TI FIELD RELEASE IN FLORIDA OF DIAPHORENCYRTUS ALIGARHENSIS (HYMENOPTERA: ENCYRTIDAE), AN ENDOPARASITOID OF DIAPHORINA CITRI (HOMOPTERA: PSYLLIDAE), FROM MAINLAND CHINA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID DISEASE C1 [Rohrig, Eric A.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Rohrig, Eric A.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Hall, David G.] USDA ARS, Hort Res Lab, Ft Pierce, FL 34945 USA. [Qureshi, Jawwad A.; Stansly, Philip A.] Univ Florida, IFAS, SWFREC, Immokalee, FL 34142 USA. RP Rohrig, EA (reprint author), Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. NR 10 TC 6 Z9 9 U1 3 U2 24 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 479 EP 481 PG 3 WC Entomology SC Entomology GA 960EL UT WOS:000305370700032 ER PT J AU Boughton, AJ Nelson, B Center, TD AF Boughton, Anthony J. Nelson, Brian Center, Ted D. TI EFFORTS TO ESTABLISH A BIOLOGICAL CONTROL AGENT AGAINST INCIPIENT INFESTATIONS OF OLD WORLD CLIMBING FERN IN SOUTHWEST FLORIDA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID CONSPURCATALIS LEPIDOPTERA CRAMBIDAE; LYGODIUM-MICROPHYLLUM; INVASIVE WEED; NEOMUSOTIMA-CONSPURCATALIS C1 [Boughton, Anthony J.; Center, Ted D.] USDA ARS, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Nelson, Brian] SW Florida Water Management Dist, Brooksville, FL 34604 USA. RP Boughton, AJ (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. NR 16 TC 1 Z9 1 U1 1 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 482 EP 484 PG 3 WC Entomology SC Entomology GA 960EL UT WOS:000305370700033 ER PT J AU Tyler-Julian, K Demirozer, O Figlar, R Skarlinsky, T Funderburk, J Knox, G AF Tyler-Julian, Kara Demirozer, Ozan Figlar, Richard Skarlinsky, Thomas Funderburk, Joe Knox, Gary TI NEW HOST RECORDS FOR CALIOTHRIPS STRIATUS (THYSANOPTERA: THRIPIDAE) ON MAGNOLIA SPP. WITH THE DESCRIPTION OF THE SECOND INSTAR SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material C1 [Tyler-Julian, Kara; Demirozer, Ozan; Funderburk, Joe; Knox, Gary] Univ Florida, Quincy, FL 32351 USA. [Figlar, Richard] Magnolian Grove Arboretum, Pickens, SC 29671 USA. [Skarlinsky, Thomas] USDA APHIS PPQ, Miami, FL 33266 USA. RP Tyler-Julian, K (reprint author), Univ Florida, 155 Res Rd, Quincy, FL 32351 USA. EM karatylerjulian@ufl.edu; odemirozer@ufl.edu; magnolia@magnoliaceae.org; Thomas.L.Skarlinsky@aphis.usda.gov; jef@ufl.edu; gwknox@ufl.edu NR 5 TC 0 Z9 1 U1 0 U2 3 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 485 EP 488 PG 4 WC Entomology SC Entomology GA 960EL UT WOS:000305370700034 ER PT J AU Valles, SM Strong, CA AF Valles, Steven M. Strong, Charles A. TI CONFIRMATION OF THE GENOME POSITION AND MASS OF THE VIRAL PROTEIN, VP3, OF SOLENOPSIS INVICTA VIRUS 1 (PICORNAVIRALES: DICISTROVIRIDAE) INFECTING THE RED IMPORTED FIRE ANT (HYMENOPTERA: FORMICIDAE) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material C1 [Valles, Steven M.; Strong, Charles A.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Valles, SM (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM Steven.Valles@ars.usda.gov NR 6 TC 0 Z9 0 U1 1 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 494 EP 496 PG 3 WC Entomology SC Entomology GA 960EL UT WOS:000305370700037 ER PT J AU Oi, DH Valles, SM Porter, SD AF Oi, David H. Valles, Steven M. Porter, Sanford D. TI THE FIRE ANT (HYMENOPTERA: FORMICIDAE) PATHOGEN, VAIRIMORPHA INVICTAE (MICROSPORIDIA: BURENELLIDAE), NOT DETECTED IN FLORIDA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID THELOHANIA-SOLENOPSAE MICROSPORIDIA; UNITED-STATES; PREVALENCE; ARGENTINA; POPULATIONS; INFECTIONS; TEXAS C1 [Oi, David H.; Valles, Steven M.; Porter, Sanford D.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Oi, DH (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM david.oi@ars.usda.gov NR 25 TC 3 Z9 3 U1 1 U2 8 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 506 EP 508 PG 3 WC Entomology SC Entomology GA 960EL UT WOS:000305370700041 ER PT J AU Oi, DH Valles, SM AF Oi, David H. Valles, Steven M. TI HOST SPECIFICITY TESTING OF THE SOLENOPSIS FIRE ANT (HYMENOPTERA: FORMICIDAE) PATHOGEN, KNEALLHAZIA (=THELOHANIA) SOLENOPSAE (MICROSPORIDIA: THELOHANIIDAE), IN FLORIDA SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID MULTIPLEX PCR; INVICTA; GROWTH C1 [Oi, David H.; Valles, Steven M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Oi, DH (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM david.oi@ars.usda.gov NR 21 TC 1 Z9 1 U1 3 U2 15 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 509 EP 512 PG 4 WC Entomology SC Entomology GA 960EL UT WOS:000305370700042 ER PT J AU Kendra, PE Montgomery, WS Sanchez, JS Deyrup, MA Niogret, J Epsky, ND AF Kendra, Paul E. Montgomery, Wayne S. Sanchez, Jorge S. Deyrup, Mark A. Niogret, Jerome Epsky, Nancy D. TI METHOD FOR COLLECTION OF LIVE REDBAY AMBROSIA BEETLES, XYLEBORUS GLABRATUS (COLEOPTERA: CURCULIONIDAE: SCOLYTINAE) SO FLORIDA ENTOMOLOGIST LA English DT Editorial Material ID ANASTREPHA-SUSPENSA DIPTERA; SOUTHEASTERN UNITED-STATES; ESSENTIAL OIL LURES; LAUREL WILT; ELECTROANTENNOGRAM RESPONSES; PHOEBE OIL; FRUIT-FLY; ATTRACTION; LAURACEAE; AVOCADO C1 [Kendra, Paul E.; Montgomery, Wayne S.; Sanchez, Jorge S.; Niogret, Jerome; Epsky, Nancy D.] USDA ARS, Subtrop Hort Res Stn, Miami, FL 33158 USA. [Deyrup, Mark A.] Archbold Biol Stn, Lake Placid, FL 33862 USA. RP Kendra, PE (reprint author), USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM paul.kendra@ars.usda.gov NR 16 TC 10 Z9 11 U1 1 U2 7 PU FLORIDA ENTOMOLOGICAL SOC PI LUTZ PA 16125 E LAKE BURRELL DR, LUTZ, FL 33548 USA SN 0015-4040 J9 FLA ENTOMOL JI Fla. Entomol. PD JUN PY 2012 VL 95 IS 2 BP 513 EP 516 PG 4 WC Entomology SC Entomology GA 960EL UT WOS:000305370700043 ER PT J AU Bunce, J AF Bunce, James TI Using New Gas Exchange Methods to Estimate Mesophyll Conductance and Non-stomatal Inhibition of Photosynthesis Caused by Water Deficits SO HORTSCIENCE LA English DT Article DE Glycine max; Phaseolus vulgaris; Brassica oleracea; Abutilon theophrasti; stomatal conductance; water stress ID CARBON-DIOXIDE; CO2 CONCENTRATION; HIGHER-PLANTS; RESPONSES; STRESS; LIMITATIONS; DIFFUSION; LEAVES AB Soil water deficits remain one of the most important factors reducing the yield of crop plants and may become even more limiting with changes in the global climate and competition for fresh water resources. Soil water deficits reduce plant growth partly by reducing photosynthesis. However, it remains unclear how important non-stomatal factors are in limiting photosynthesis under moderate water stress and whether rising atmospheric carbon dioxide may alter which processes limit photosynthesis under water stress. The conductance to CO2 from the substomatal air space to the site of carboxylation inside chloroplasts in C-3 plants is now termed mesophyll conductance. Because of the competition between CO2 and O-2 for RuBisco, the carbon dioxide concentration at the chloroplast can be estimated from the O-2 sensitivity of photosynthesis, providing a new method of estimating mesophyll conductance. It has also recently been realized that partial stomatal closure resulting from water stress can often be reversed by exposing leaves to low CO2. This provides a new means of assessing the non-stomatal component of the inhibition of photosynthesis by water stress. These methods were applied to four C-3 species and revealed that mesophyll conductance decreased substantially with water stress in two of the four species and that reopening of stomata did not eliminate the reduction in photosynthesis caused by moderate water stress at either the current ambient or elevated CO2 concentrations. C1 ARS, Crop Syst & Global Change Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Bunce, J (reprint author), ARS, Crop Syst & Global Change Lab, USDA, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM James.Bunce@ars.usda.gov FU Environmental Stress Physiology (SIRS) Working Group FX This paper was part of the colloquium, "Emerging Techniques to Evaluate and Mitigate Crop Environmental Stress in a Changing Climate" held 28 Sept. 2011 at the ASHS Conference, Waikoloa, HI, and sponsored by the Environmental Stress Physiology (SIRS) Working Group. NR 18 TC 2 Z9 2 U1 2 U2 17 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 687 EP 690 PG 4 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900002 ER PT J AU Glenn, DM AF Glenn, D. Michael TI Infrared and Chlorophyll Fluorescence Imaging Methods for Stress Evaluation SO HORTSCIENCE LA English DT Article DE thermography; differential thermal analysis; green fluorescent protein; reactive oxygen species; charged coupled device camera ID LEAVES; PLANTS; PHOTOSYNTHESIS; THERMOGRAPHY; HERBIVORY AB Infrared and chlorophyll fluorescence imaging methods are useful techniques to evaluate environmental effects on plant performance. With the advent of digital imaging and advances in sensor technology, infrared (IR) thermography has become more accurate and less expensive. Modern IR cameras can resolve 0.5 degrees C temperature differences and research-grade instruments can resolve 0.05 degrees C. This precision has allowed the physical processes of freezing and transpiration to be more accurately studied and modeled. Chlorophyll fluorescence imaging, although still an expensive technology, has also become sufficiently rugged to be useful in the field. The measurement of quantum efficiency, Fv/Fm, provides clear data on the effect of various environmental and biotic effects on the performance of photosynthesis in plants through the effect on photosystem II. Modern digital cameras with low signal-to-noise ratios can also image chlorophyll fluorescence using time lapse exposure. Peltier-cooled charge coupled device (CCD) cameras can measure the autoluminescence in stressed plants that is generated by reactive oxygen species. Advances in technology have reduced the cost and precision of imaging equipment to a point that they are more applicable tools to plant scientists. C1 ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenn@ars.usda.gov FU Environmental Stress Physiology (STRS) Working Group FX This paper was part of the colloquium, "Emerging Techniques to Evaluate and Mitigate Crop Environmental Stress in a Changing Climate" held 28 Sept. 2011 at the ASHS Conference, Waikoloa, HI, and sponsored by the Environmental Stress Physiology (STRS) Working Group. NR 20 TC 7 Z9 8 U1 1 U2 24 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 697 EP 698 PG 2 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900004 ER PT J AU Glenn, DM AF Glenn, D. Michael TI The Mechanisms of Plant Stress Mitigation by Kaolin-based Particle Films and Applications in Horticultural and Agricultural Crops SO HORTSCIENCE LA English DT Article DE heat stress; ultraviolet radiation; photosynthetically active radiation; water stress; whole canopy photosynthesis ID WATER-USE EFFICIENCY; FRUIT-QUALITY; EMPIRE APPLE; DIPTERA TEPHRITIDAE; SUNBURN; PHOTOSYNTHESIS; CANOPIES; ORCHARDS; REDUCE; CITRUS AB Kaolin-based particle films have use in reducing insect, heat, photosynthetically active radiation (PAR), and ultraviolet radiation stress in plants resulting from the reflective nature of the particles. Particle films with a residue density of 1 to 4 g.m(-2) have been evaluated in a range of crops and agricultural environments. The particle film is a general insect repellant resulting from the change in the plant's leaf/fruit texture but also because it changes the reflected light signature of the plant causing insect avoidance for many pests. The alteration of reflected light is the result of the ability of the particle film to reflect infrared (IR), PAR, and ultraviolet radiation. Reflection of IR can reduce canopy temperature as much as 5 inverted perpendicular C, which will reduce potential transpiration. The reduction of PAR by the film at the leaf level is compensated in varying degrees by diffusion of PAR into the interior of the canopy. Whole canopy photosynthesis can be increased by the combined particle film effects of reduced canopy temperature and increased diffusion of PAR into the interior of the canopy. In apple, reducing fruit surface temperature, PAR, and ultraviolet is an effective means of reducing sunburn damage. The use of a reflective particle film is effective in mitigating environmental stress and has significant economic benefits in agricultural crops. C1 ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Glenn, DM (reprint author), ARS, USDA, Appalachian Fruit Res Stn, 2217 Wiltshire Rd, Kearneysville, WV 25430 USA. EM michael.glenne@ars.usda.gov FU Environmental Stress Physiology (STRS) Working Group FX This paper was part of the colloquium, "Emerging Techniques to Evaluate and Mitigate Crop Environmental Stress in a Changing Climate" held 28 Sept. 2011 at the ASHS Conference, Waikoloa, HI, and sponsored by the Environmental Stress Physiology (STRS) Working Group. NR 29 TC 4 Z9 4 U1 2 U2 26 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 710 EP 711 PG 2 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900007 ER PT J AU Sriladda, C Kratsch, HA Larson, SR Kjelgren, RK AF Sriladda, Chalita Kratsch, Heidi A. Larson, Steven R. Kjelgren, Roger K. TI Morphological and Genetic Variation among Four High Desert Sphaeralcea Species SO HORTSCIENCE LA English DT Article DE Sphaeralcea coccinea; Sphaeralcea grossulariifolia; Sphaeralcea munroana; Sphaeralcea parvifolia; globemallow; AFLP ID MULTILOCUS GENOTYPE DATA; COLORADO PLATEAU; WATER; INFERENCE AB The herbaceous perennial species in the genus Sphaeralcea have desirable drought tolerance and aesthetics with potential for low-water use landscapes in the Intermountain West. However, taxonomy of these species is ambiguous, which leads to decreased consumer confidence in the native plant nursery industry. The goal of this study was to test and clarify morphological and genetic differentiation among four putative Sphaeralcea species. Morphological characteristics of the type specimens were used as species references in canonical variate analysis to generate a classification model. This model was then used to assign putative species names to herbarium voucher specimens and to field-collected voucher specimens to clarify genetic variation among species. Field specimens were also classified using Bayesian cluster analyses of amplified fragment length polymorphism (AFLP) genotypes. Sphaeralcea coccinea (Nutt.) Rydb. and S. grossulariifolia (Hook. & Ant.) Rydb. formed a composite group morphologically and genetically distinct from the S. munroana (Douglas) Spach and S. parvifolia A. Nelson composite group. Each composite group displayed genetic isolation by geographic distance. Also, morphological traits of S. munroana and S. parvifolia correlated to geographic distance. Taken together these results suggest that our samples represent two sympatric yet reproductively isolated groups. Distinguishing between these two Sphaeralcea composite groups can create greater consumer confidence in plant material developed for use in Intermountain West low-water landscaping. C1 [Sriladda, Chalita; Kjelgren, Roger K.] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. [Kratsch, Heidi A.] Univ Nevada, Univ Nevada Cooperat Extens, Reno, NV 89502 USA. [Larson, Steven R.] Utah State Univ, Forage & Range Res Lab, USDA, ARS, Logan, UT 84322 USA. RP Sriladda, C (reprint author), Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. EM sriladda@gmail.com NR 29 TC 0 Z9 0 U1 2 U2 13 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 715 EP 720 PG 6 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900009 ER PT J AU Panthee, DR Cao, CX Debenport, SJ Rodriguez, GR Labate, JA Robertson, LD Breksa, AP van der Knaap, E Gardener, BBM AF Panthee, Dilip R. Cao, Chunxue Debenport, Spencer J. Rodriguez, Gustavo R. Labate, Joanne A. Robertson, Larry D. Breksa, Andrew P., III van der Knaap, Esther Gardener, Brian B. McSpadden TI Magnitude of Genotype x Environment Interactions Affecting Tomato Fruit Quality SO HORTSCIENCE LA English DT Article DE heritability; lycopene; Solanum lycopersieum; total soluble solids; vitamin C ID FRESH-MARKET TOMATO; ORGANOLEPTIC QUALITY; LYCOPERSICON-ESCULENTUM; GENETIC BACKGROUNDS; LYCOPENE CONTENT; MAPPING QTLS; TRAITS; LINES; INTROGRESSION; POPULATIONS AB There is a growing interest by consumers to purchase fresh tomatoes with improved quality traits including lycopene, total soluble solids (TSS), vitamin C, and total titratable acid (TTA) content. As a result, there are considerable efforts by tomato breeders to improve tomato for these traits. However, suitable varieties developed for one location may not perform the same in different locations. This causes a problem for plant breeders because it is too labor-intensive to develop varieties for each specific location. The objective of this study was to determine the extent of genotype x environment (GxE) interaction that influences tomato fruit quality. To achieve this objective, we grew a set of 42 diverse tomato genotypes with different fruit shapes in replicated trials in three locations: North Carolina, New York, and Ohio. Fruits were harvested at the red ripe stage and analyzed for lycopene, TSS, vitamin C, and TTA. Analysis of variance (ANOVA) revealed that there were significant differences (P < 0.05) among tomato genotypes, locations, and their interaction. Further analysis of quality traits from individual locations revealed that there was as much as 211% change in performance of some genotypes in a certain location compared with the average performance of a genotype. Lycopene was found to be most influenced by the environment, whereas TTA was the least influenced. This was in agreement with heritability estimates observed in the study for these quality traits, because heritability estimate for lycopene was 16%, whereas that for TTA was 87%. The extent of GxE interaction found for the fruit quality traits in the tomato varieties included in this study may be useful in identifying optimal locations for future field trials by tomato breeders aiming to improve tomato fruit quality. C1 [Panthee, Dilip R.] N Carolina State Univ, Dept Hort Sci, Mt Hort Crops Res & Extens Ctr, Mills River, NC 28759 USA. [Cao, Chunxue; Debenport, Spencer J.; Gardener, Brian B. McSpadden] Ohio State Univ, Dept Plant Pathol, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Rodriguez, Gustavo R.; van der Knaap, Esther] Ohio State Univ, Dept Hort & Crop Sci, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Labate, Joanne A.; Robertson, Larry D.] ARS, USDA, Plant Genet Resources Unit, Geneva, NY 14456 USA. [Breksa, Andrew P., III] ARS, USDA, Processed Foods Res Unit, Albany, CA 94710 USA. RP Panthee, DR (reprint author), N Carolina State Univ, Dept Hort Sci, Mt Hort Crops Res & Extens Ctr, 455 Res Dr, Mills River, NC 28759 USA. EM Dilip_panthee@ncsu.edu RI van der Knaap, Esther/D-6307-2012 FU National Science Foundation Biological Infrastructure Program [DBI-0922661]; CRIS [1910-21000-019-00D, 5325-41430-011-00D] FX This study was supported in part by National Science Foundation Biological Infrastructure Program, Award Number DBI-0922661, and CRIS Project no. 1910-21000-019-00D and 5325-41430-011-00D. NR 32 TC 6 Z9 6 U1 0 U2 16 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 721 EP 726 PG 6 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900010 ER PT J AU Miller, B Bruck, DJ Walton, V AF Miller, Betsey Bruck, Denny J. Walton, Vaughn TI Relationship of Black Vine Weevil Egg Density and Damage to Two Cranberry Cultivars SO HORTSCIENCE LA English DT Article DE Otiorhynchus sulcants; Vacciniun macrocarpon; root damage; leaf area; Pacific Northwest; 'Stevens'; 'McFarlin' ID OTIORHYNCHUS-SULCATUS; ROOT; GROWTH; CURCULIONIDAE; COLEOPTERA; WASHINGTON; STRAWBERRY; NITROGEN; PLANTS AB Black vine weevil (BVW), Otiorhynchus sulcatus (Coleoptera: Curculionidae, Fabricius), is a serious pest of cranberry, Vaccinium macrocarpon Ait. Larvae feed undetected within the soil and cause damage to roots and underground vines. We correlated damage caused by feeding larvae from known BVW egg densities. Two cultivars of potted cranberry vines, 'Stevens' and 'McFarlin', were inoculated with 0, 5, 10, 20, 40, and 80 eggs per pot. Root damage and canopy health were assessed. 'Stevens' exceeded 'McFarlin' in dry shoot weight, total shoot length, total leaf area, and dry root weight before egg treatments. Damage to underground vines increased with increasing egg density and more damage was found in 'Stevens' than 'McFarlin' at the highest egg densities. In August, plant water use and total shoot length in 'McFarlin' were significantly greater in plants treated with 0-5 eggs per pot compared with plants treated with 40-80 eggs per pot. The effect on total shoot length was more pronounced in October. 'Stevens' showed no response to increasing BVW density for up to 24 weeks. Destructive measurements showed decreased root weight in 'McFarlin' but not 'Stevens'. Both cultivars showed a similar decrease in dry shoot weight, total shoot length, and percent green leaf area with increasing BVW egg density. Root damage increased as BVW egg density increased and this damage resulted in reduced plant water use for 'McFarlin'. Reduced shoot growth and leaf area was recorded for both cultivars, although these effects were more apparent in 'McFarlin' and at an earlier stage than in 'Stevens'. C1 [Miller, Betsey; Walton, Vaughn] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Bruck, Denny J.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97331 USA. RP Miller, B (reprint author), Oregon State Univ, Dept Hort, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. EM betsey.miller@hort.oregonstate.edu FU Agricultural Research Foundation; Oregon Cranberry Growers Association FX We thank the Agricultural Research Foundation and the Oregon Cranberry Growers Association for funding this study. Thanks to Jim Oliphant, Kim Patten, Inga Zasada, Dave Bryla, Dave Smith, Caroline Scagel, Paul Charron, and Amy Dreves for consultation and materials. Special thanks to Kevin Talbot and Knute Andersson for providing plant material and to Amanda Lake for rearing the insects used in this study. Special thanks to Mike and Lori Reitmajer, Drew Mahedy, Tyler Kilkenny, and Sam Tochen for assistance with data collection. Thanks to Daniel Dalton, Linda White, and anonymous reviewers for editing earlier versions of the manuscript. NR 44 TC 0 Z9 0 U1 0 U2 10 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 755 EP 761 PG 7 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900016 ER PT J AU Sakhanokho, HF Witcher, AL Pounders, CT Spiers, JM AF Sakhanokho, Hamidou F. Witcher, Anthony L. Pounders, Cecil T. Spiers, James M. TI 'Ramata': A New Dwarf Variegated Hedychium (Ornamental Ginger) Cultivar SO HORTSCIENCE LA English DT Editorial Material DE ginger lilies; Hedychium muluense; somaclonal variation; variegated foliage ID GENOME SIZE C1 [Sakhanokho, Hamidou F.; Witcher, Anthony L.; Pounders, Cecil T.; Spiers, James M.] ARS, USDA, Thad Cochran So Hort Lab, Poplarville, MS 39470 USA. RP Sakhanokho, HF (reprint author), ARS, USDA, Thad Cochran So Hort Lab, 810 Highway 26 W, Poplarville, MS 39470 USA. EM Hamidou.Sakhanokho@ars.usda.gov NR 12 TC 1 Z9 1 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUN PY 2012 VL 47 IS 6 BP 803 EP 805 PG 3 WC Horticulture SC Agriculture GA 960CZ UT WOS:000305366900025 ER PT J AU Eigenberg, RA Woodbury, BL AF Eigenberg, Roger A. Woodbury, Bryan L. TI Using Resistivity Arrays to Monitor Groundwater Impacts near Runoff Holding Ponds SO JOURNAL OF ENVIRONMENTAL AND ENGINEERING GEOPHYSICS LA English DT Article ID ANIMAL WASTE; SEAL FORMATION; SEEPAGE; MANURE; EVAPORATION; LAGOONS; LOSSES; SOILS AB Mineral and organic salts from beef manure contained in precipitation runoff from feedyard pen surfaces can alter the conductivity properties of soil and water receiving it. Typically, holding ponds are constructed to control runoff from concentrated animal feeding operations. The integrity of these holding ponds has come under increased scrutiny since leakage has the potential to affect soil and groundwater quality. Traditionally, ponds are monitored by installing monitoring wells at key locations to evaluate the impact of these ponds on the environment. These monitoring wells are expensive and subject to ambiguous interpretation. A subsurface resistivity array was installed at a beef cattle feedyard located at the U.S Meat Animal Research Center, Clay Center, Nebraska (Feedyard A) and at a cattle feeding cooperator site (Feedyard B). Array probes were permanently installed at Feedyard A (16 probes spaced 6.1-m apart at a depth of 30 cm) and at Feedyard B (32 probes spaced 3.05-m apart at a depth of 50 cm). Weekly readings from each site were evaluated to monitor the stability of the zone of hydration near the pond. The low hydraulic conductivity soils at Feedyard A provided a very quiescent environmental system to evaluate the resistivity array's inherent measurement stability. Seasonal changes could easily be accounted for by variation in seasonal soil temperatures. The Feedyard B site was typified by coarse textured parent material that had high hydraulic conductivity properties. This site experienced dynamic changes week to week and throughout the season. The resistivity array system was able to adequately measure these dynamics. Additional analysis using difference maps improved the illustration of the resulting dynamic. The results of this study indicate that the resistivity array system has the potential to improve monitoring of runoff holding ponds and warrants additional validation. C1 [Eigenberg, Roger A.; Woodbury, Bryan L.] USDA ARS, USMARC, Dept Environm Management Res Unit, Clay Ctr, NE 68933 USA. RP Eigenberg, RA (reprint author), USDA ARS, USMARC, Dept Environm Management Res Unit, Clay Ctr, NE 68933 USA. EM roger.eigenberg@ars.usda.gov; bryan.woodbury@ars.usda.gov FU University of Nebraska; AgraTek Corp. FX The research was conducted in cooperation with the Nebraska Department of Environmental Quality, The Nebraska Cattlemen Association and with the support of University of Nebraska and AgraTek Corp. NR 21 TC 2 Z9 2 U1 0 U2 7 PU ENVIRONMENTAL ENGINEERING GEOPHYSICAL SOC PI DENVER PA 1720 SOUTH BELLAIRE, STE 110, DENVER, CO 80222-433 USA SN 1083-1363 J9 J ENVIRON ENG GEOPH JI J. Environ. Eng. Geophys. PD JUN PY 2012 VL 17 IS 2 BP 103 EP 112 PG 10 WC Geochemistry & Geophysics; Engineering, Geological SC Geochemistry & Geophysics; Engineering GA 961EO UT WOS:000305447000004 ER PT J AU Cantrell, CL Dayan, FE Duke, SO AF Cantrell, Charles L. Dayan, Franck E. Duke, Stephen O. TI Natural Products As Sources for New Pesticides SO JOURNAL OF NATURAL PRODUCTS LA English DT Review ID P-HYDROXYPHENYLPYRUVATE DIOXYGENASE; CORN GLUTEN HYDROLYSATE; CHAIN FATTY-ACIDS; BOTRYTIS-CINEREA; MEAL; INSECTICIDES; HERBICIDE; GROWTH; MODE; GERMINATION AB Natural products as pesticides have been reviewed from several perspectives in the past, but no prior treatment has examined the impact of natural product and natural product-based pesticides on the U.S. market, as a function of new active ingredient registrations with the Environmental Protection Agency (EPA). Thus, EPA registration details of new active ingredients for all conventional pesticide registrations and biopesticide registrations were compiled from the years 1997-2010. Conventional pesticide registrations and biopesticide registrations were examined both collectively and independently for all 277 new active ingredients (NAI) and subsequently categorized and sorted into four types: biological (B), natural product (NP), synthetic (5), and synthetic natural derived (SND). When examining conventional pesticides alone, the S category accounted for the majority of NAI registrations, with 78.0%, followed by SND with 14.7%, NP with 6.4%, and B with 0.9%. Biopesticides alone were dominated by NPs with 54.8%, followed by B with 44.6%, SND with 0.6%, and 0% for S. When examining conventional pesticides and biopesticides combined, NPs accounted for the majority of NAI registrations, with 35.7%, followed by S with 30.7%, B with 27.4%, and SND with 6.1%. Despite the common perception that natural products may not be the best sources for NAI as pesticides, when both conventional and biopesticides are examined collectively, and considering that NP, SND, and B all have origins from natural product research, it can be argued that their combined impact with the EPA from 1997 to 2010 accounted for 69.3% of all NAI registrations. C1 [Cantrell, Charles L.; Dayan, Franck E.; Duke, Stephen O.] ARS, Nat Prod Utilizat Res Unit, USDA, University, MS 38677 USA. RP Cantrell, CL (reprint author), ARS, Nat Prod Utilizat Res Unit, USDA, POB 8048, University, MS 38677 USA. EM charles.cantrell@ars.usda.gov RI Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 FU Deployed War-Fighter Protection Research Program Grant; U.S. Department of Defense through the Armed Forces Pest Management Board FX We thank A. Reichley for technical assistance. This review was supported, in part, by the Deployed War-Fighter Protection Research Program Grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. NR 67 TC 113 Z9 119 U1 11 U2 145 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 J9 J NAT PROD JI J. Nat. Prod. PD JUN PY 2012 VL 75 IS 6 BP 1231 EP 1242 DI 10.1021/np300024u PG 12 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA 961XT UT WOS:000305503500035 PM 22616957 ER PT J AU Hwang, HS Winkler-Moser, JK Liu, SX AF Hwang, Hong-Sik Winkler-Moser, Jill K. Liu, Sean X. TI Structural Effect of Lignans and Sesamol on Polymerization of Soybean Oil at Frying Temperature SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Lignan; Soybean oil; Frying oil; Antioxidant; Synthetic antioxidant; Antipolymerization; Polymerized triacylglycerol; Radical scavenging; Natural antioxidant ID NUCLEAR-MAGNETIC-RESONANCE; FREE-RADICAL SCAVENGERS; FATTY-ACID-COMPOSITION; EDIBLE VEGETABLE-OILS; ANTIOXIDANT ACTIVITY; THERMAL-STABILITY; NMR; CELLS; PLANT AB The antipolymerization activity of the lignans including nordihydroguaiaretic acid, (+)-pinoresinol, (-)-secoisolariciresinol, enterodiol, and two sesame lignans (sesamol and sesamin), and four model compounds (4-ethylcatechol, 2-methoxy-4-propylphenol, 4-methyl-2,6-dimethoxyphenol, and piperonyl alcohol) were investigated for soybean oil at frying temperature (180 degrees C). The heated samples were analyzed by gel-permeation chromatography for polymerized triacylglycerols and by H-1 NMR to monitor the decrease in peak intensities for bisallylic protons and olefinic protons. The heating test results were compared with ability to scavenge the radical 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) to understand the relationship between radical-scavenging ability at room temperature and antipolymerization activity at 180 degrees C. It was found that the antipolymerization activity of a lignan at frying temperature cannot be predicted solely from the radical-scavenging ability at room temperature. The lignans tested in this research had antipolymerization activity, but it was not as strong as that of 200 ppm tert-butylhydroquinone (TBHQ) with equivalent of phenolic moiety (0.0012 meq/g). At higher concentrations, however, sesamol, nordihydroguaiaretic acid, and (+)-pinoresinol had better antipolymerization activity than the legal limit set for TBHQ (200 ppm, 0.0012 meq/g). Therefore, this research showed that by using an appropriate concentration, these natural antioxidants can replace synthetic antioxidants and provide better antioxidation activity. C1 [Hwang, Hong-Sik; Winkler-Moser, Jill K.; Liu, Sean X.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Hwang, HS (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Hongsik.hwang@ars.usda.gov NR 30 TC 16 Z9 16 U1 4 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2012 VL 89 IS 6 BP 1067 EP 1076 DI 10.1007/s11746-011-1994-6 PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 959DY UT WOS:000305293600013 ER PT J AU Bantchev, GB Biresaw, G Cermak, SC AF Bantchev, Grigor B. Biresaw, Girma Cermak, Steven C. TI Elastohydrodynamic Study of Blends of Bio-Based Esters with Polyalphaolefin in the Low Film Thickness Regime SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Elastohydrodynamics; Vegetable oil; Thin film; Polyalphaolefin; PAO; Blends; Soybean oil; Estolide; Bio-based lubricant; Fractionation; Boundary film formation; Elastohydrodynamic film thickness; Hamrock-Dowson model ID THIN LIQUID-FILMS; FORMING PROPERTIES; LUBRICANT FILMS; OIL; FRICTION; SURFACES; DRAINAGE AB The film thickness in elastohydrodynamic (EHD) conditions for soybean oil (SBO), oleic estolide ester (EST) and their binary blends with polyalphaolefins (PAO2 or PAO40) were studied at 30 and 100 degrees C. Changes with time, for up to 200 min, were monitored. SBO and its blends with the lower viscosity PAO2 showed initially good agreement with the Hamrock-Dowson (H-D) equation down to 1-3 nm film thickness. 60 min or more after the start of the measurements, boundary layers with thickness up to 4.7 nm were observed. The blend of SBO with the more viscous PAO40 showed initially a good agreement with H-D at 100 degrees C. Negative deviations in film thickness were observed 15 min after the start of the measurements. At extended periods of time, up to 200 min, they were less pronounced but still detectable. EST-PAO2 blend showed initially formation of boundary layers with thickness around 2 nm. The boundary layer at 30 degrees C did not change for 200 min, while at 100 degrees C showed a decrease in thickness and/or viscosity with time. The EST and the EST-PAO40 blends showed good agreement with the H-D equation and did not display a boundary or fractionation layer within 200 min. C1 [Bantchev, Grigor B.; Biresaw, Girma; Cermak, Steven C.] ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Bantchev, GB (reprint author), ARS, Biooils Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM Grigor.Bantchev@ars.usda.gov; Girma.Biresaw@ars.usda.gov; Steven.Cermak@ars.usda.gov OI Bantchev, Grigor/0000-0003-2790-5195 NR 25 TC 1 Z9 1 U1 0 U2 9 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD JUN PY 2012 VL 89 IS 6 BP 1091 EP 1099 DI 10.1007/s11746-011-1988-4 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 959DY UT WOS:000305293600015 ER PT J AU Kim, SP Kang, MY Nam, SH Friedman, M AF Kim, Sung Phil Kang, Mi Young Nam, Seok Hyun Friedman, Mendel TI Dietary rice bran component gamma-oryzanol inhibits tumor growth in tumor-bearing mice SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Angiogenic; Inflammatory; gamma-Oryzanol; Rice bran; Tumor growth inhibition ID FERMENTED BROWN RICE; ANTIOXIDANT ACTIVITY; NITRIC-OXIDE; CANCER CELLS; HULL SMOKE; EXTRACTS; TUMORIGENESIS; INFLAMMATION; APOPTOSIS; OIL AB Scope: We investigated the effects of rice bran and components on tumor growth in mice. Methods and results: Mice fed standard diets supplemented with rice bran, gamma-oryzanol, Ricetrienol (R), ferulic acid, or phytic acid for 2 weeks were inoculated with CT-26 colon cancer cells and fed the same diet for two additional weeks. Tumor mass was significantly lower in the gamma-oryzanol and less so in the phytic acid group. Tumor inhibition was associated with the following biomarkers: increases in cytolytic activity of splenic natural killer (NK) cells; partial restoration of nitric oxide production and phagocytosis in peritoneal macrophages increases in released the pro-inflammatory cytokines tumor necrosis factor-alpha, IL-1 beta, and IL-6 from macrophages; and reductions in the number of blood vessels inside the tumor. Pro-angiogenic biomarkers vascular endothelial growth factor (VEGF), cyclooxygenase-2 (COX-2), and 5-lipoxygenase-5 (5-LOX) were also significantly reduced in mRNA and protein expression by tumor genes. ELISA of tumor cells confirmed reduced expression of COX-2 and 5-LOX up to 30%. Reduced COX-2 and 5-LOX expression downregulated VEGF and inhibited neoangiogenesis inside the tumors. Conclusion: Induction of NK activity, activation of macrophages, and inhibition of angiogenesis seem to contribute to the inhibitory mechanism of tumor regression by gamma-oryzanol. C1 [Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Kim, Sung Phil] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea. [Kang, Mi Young] Kyungpook Natl Univ, Dept Food Sci & Nutr, Taegu, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. RP Nam, SH (reprint author), Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. EM SHNam@ajou.ac.kr; Mendel.Friedman@ars.usda.gov NR 47 TC 31 Z9 34 U1 4 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD JUN PY 2012 VL 56 IS 6 BP 935 EP 944 DI 10.1002/mnfr.201200057 PG 10 WC Food Science & Technology SC Food Science & Technology GA 959OU UT WOS:000305323100010 PM 22707268 ER PT J AU Davis, TS Landolt, PJ AF Davis, Thomas Seth Landolt, Peter J. TI Body size phenotypes are heritable and mediate fecundity but not fitness in the lepidopteran frugivore Cydia pomonella SO NATURWISSENSCHAFTEN LA English DT Article DE Codling moth; Cydia pomonella; Body size; Fecundity; Phenotype; Heritability ID MOTH UTETHEISA-ORNATRIX; FEMALE CODLING MOTH; DISRUPTIVE SELECTION; L. LEPIDOPTERA; NUPTIAL GIFT; TRADE-OFF; TORTRICIDAE; MAMMALS; COMPETITION; DROSOPHILA AB The inheritance and functional roles of quantitative traits are central concerns of evolutionary ecology. We report two sets of experiments that investigated the heritability and reproductive consequences of body size phenotypes in a globally distributed lepidopteran frugivore, Cydia pomonella (L.). In our first set of experiments, we tested the hypotheses that (1) body size is heritable and (2) parental body size mediates egg production and offspring survival. Midparent-offspring regression analyses revealed that body mass is highly heritable for females and moderately heritable for males. The contribution of fathers to estimates of additive genetic variance was slightly greater than for mothers. Egg production increased with mean parental size, but offspring survival rates were equivalent. Based on this result, we tested two additional hypotheses in a second set of experiments: (3) male size moderates female egg production and egg fertility and (4) egg production, egg fertility, and offspring survival rate are influenced by female mating opportunities. Females paired with large males produced more eggs and a higher proportion of fertile eggs than females paired with small males. Females with multiple mating opportunities produced more fertile eggs than females paired with a single male. However, egg production and offspring survival rates were unaffected by the number of mating opportunities. Our experiments demonstrate that body mass is heritable in C. pomonella and that size phenotypes may mediate fecundity but not fitness. We conclude that male size can influence egg production and fertility, but female mate choice also plays a role in determining egg fertility. C1 [Davis, Thomas Seth; Landolt, Peter J.] USDA ARS, Wapato, WA 98951 USA. RP Davis, TS (reprint author), USDA ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM tsdavis1@gmail.com NR 48 TC 4 Z9 4 U1 6 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0028-1042 EI 1432-1904 J9 NATURWISSENSCHAFTEN JI Naturwissenschaften PD JUN PY 2012 VL 99 IS 6 BP 483 EP 491 DI 10.1007/s00114-012-0923-3 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 961OV UT WOS:000305476200006 PM 22584427 ER PT J AU Curtiss, J Turley, RB Stewart, JM Zhang, JF AF Curtiss, J. Turley, R. B. Stewart, J. McD. Zhang, J. F. TI Identification of Differentially Expressed Genes in Semigametic Pima Cotton by Differential Display SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Cotton; Semigamy; DDRT-PCR; cDNA-SSCP ID HOT BORATE METHOD; SUCROSE SYNTHASE; FIBER DEVELOPMENT; PHOSPHATIDIC-ACID; PHOSPHOLIPASE-D; FERTILIZATION; PROTEIN; PLANTS; CDNA; RNA AB Haploid seed-producing semigamy in cotton is controlled by a single incompletely dominant gene (Se). However, its molecular mechanism is currently poorly understood. In this study, the mRNA differential display (DD) was employed to compare gene expression between non-semigametic Pima S-1 (Gossypium barbadense) and its naturally occurring semigametic mutant 57-4. Of the 70 DD cDNA fragments isolated from a total of 3,000 cDNA fragments, some exhibited semigamy-specific expression across anthers and/or ovules. Of the 61 cDNA fragments cloned and sequenced, nine showed high homology to genes encoding for alpha-tubulin, SPK1, Rab11, sucrose synthase, isocitrate lyase, phosphoethanolamine N-methyl-transferase, phospholipase D, MADS 7, and WRKY 2. From cDNA-single-strand conformational polymorphism using primers designed from DD sequences, qualitative expression differences in six genes between the semigametic and non-semigametic genotypes were validated. However, a primer pair designed from an alpha-tubulin gene sequence yielded PCR products with quantitative difference in band intensity, which is linked to the semigamy gene at a recombination frequency of 0.316 in a (57-4xPima S-1) x Pima S-1 BC1F1 population. Its expression in the semigametic 57-4 anthers was suppressed by 2-fold as compared to that in Pima S-1 using a quantitative reverse transcription PCR analysis. This study identified several novel genes that were missed in prior microarray analysis, and their potential roles with regards to reproductive biology are discussed. C1 [Curtiss, J.; Zhang, J. F.] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Turley, R. B.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Stewart, J. McD.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. RP Zhang, JF (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Box 30003, Las Cruces, NM 88003 USA. EM jinzhang@nmsu.edu FU Arkansas Agriculture Experiment Station; New Mexico Agricultural Experiment Station; USDA-ARS [6400-21000-031-00D] FX We thank Drs. Champa Sengupta-Gopalan and Suman Bagga, Department of Plant and Environmental Sciences, New Mexico State University, for allowing us to use their iQ5 real-time thermal cycler. This research was funded in part by the Arkansas Agriculture Experiment Station, the New Mexico Agricultural Experiment Station, and the USDA-ARS Project 6400-21000-031-00D. Mention of trade names of commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the United States Department of Agriculture. NR 63 TC 6 Z9 6 U1 1 U2 13 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD JUN PY 2012 VL 30 IS 3 BP 643 EP 653 DI 10.1007/s11105-011-0378-2 PG 11 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 961PI UT WOS:000305477900014 ER PT J AU Ahern, RG Griffin, RL AF Ahern, Robert G. Griffin, Robert L. TI Untitled SO RISK ANALYSIS LA English DT Letter ID PLANTS C1 [Ahern, Robert G.; Griffin, Robert L.] USDA, APHIS, CPHST, PERAL, Washington, DC USA. RP Ahern, RG (reprint author), 1730 Vars Dr,Ste 300, Raleigh, NC 27606 USA. EM robert.g.ahern@usda.gov NR 6 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0272-4332 J9 RISK ANAL JI Risk Anal. PD JUN PY 2012 VL 32 IS 6 BP 949 EP 950 DI 10.1111/j.1539-6924.2012.01816.x PG 2 WC Public, Environmental & Occupational Health; Mathematics, Interdisciplinary Applications; Social Sciences, Mathematical Methods SC Public, Environmental & Occupational Health; Mathematics; Mathematical Methods In Social Sciences GA 960NL UT WOS:000305395100003 PM 22708692 ER PT J AU Garland, MA Moore, G AF Garland, Mark A. Moore, Gerry TI xHesperotropsis, a new nothogenus for intergeneric crosses between Hesperocyparis and Callitropsis (Cupressaceae), and a review of the complicated nomenclatural history of the Leyland cypress SO TAXON LA English DT Article DE Callitropsis; Cupressaceae; Hesperocyparis; xHesperotropsis; Leyland cypress; nomenclature of nothotaxa ID CIRCUMSCRIPTION; EVOLUTION; GENUS AB The widely cultivated Leyland cypress, a hybrid between Nootka cypress and Monterey cypress that was originally named Cupressus xleylandii, exemplifies the instability of names of nothogenera when there are changes in generic placement of the parental taxa. Because of recent phylogenetic research, the parent species of Leyland cypress have each been put in four different genera, leading to 13 possible nothospecific names for this hybrid. Here we present the correct nomenclature for the Leyland cypress under these different taxonomic concepts. We also propose a new nothogenus, xHesperotropsis, for crosses between Hesperocyparis and Callitropsis, and publish three new combinations in xHesperotropsis, including xHesperotropsis leylandii for the Leyland cypress. C1 [Garland, Mark A.; Moore, Gerry] USDA, Natl Plant Data Team, Nat Resources Conservat Serv, Greensboro, NC 27401 USA. RP Garland, MA (reprint author), USDA, Natl Plant Data Team, Nat Resources Conservat Serv, 2901 E Lee St,Suite 2100, Greensboro, NC 27401 USA. EM mark.garland@gnb.usda.gov NR 22 TC 4 Z9 4 U1 0 U2 1 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD JUN PY 2012 VL 61 IS 3 BP 667 EP 670 PG 4 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 961BO UT WOS:000305439100015 ER PT J AU Epron, D Nouvellon, Y Ryan, MG AF Epron, Daniel Nouvellon, Yann Ryan, Michael G. TI Introduction to the invited issue on carbon allocation of trees and forests SO TREE PHYSIOLOGY LA English DT Editorial Material ID HIGH TEMPORAL RESOLUTION; OF-THE-ART; BEECH TREES; SESSILE OAK; CARBOHYDRATE RESERVES; SEASONAL-CHANGES; ISOTOPE SIGNALS; RUBBER TREES; FINE-ROOT; STAND C1 [Epron, Daniel] Univ Lorraine, UMR 1137, Fac Sci, F-54500 Vandoeuvre Les Nancy, France. [Epron, Daniel] INRA, Ctr Nancy, UMR 1137, F-54280 Seichamps, France. [Epron, Daniel; Nouvellon, Yann] CIRAD, UMR 111, F-34060 Montpellier, France. [Nouvellon, Yann] Univ Sao Paulo, ESALQ, Dept Ciencias Atmosfer, IAG, BR-05508900 Sao Paulo, Brazil. [Ryan, Michael G.] US Forest Serv, USDA, Rocky Mt Res Stn, Ft Collins, CO 80521 USA. [Ryan, Michael G.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. RP Epron, D (reprint author), Univ Lorraine, UMR 1137, Fac Sci, F-54500 Vandoeuvre Les Nancy, France. EM daniel.epron@univ-lorraine.fr RI Ryan, Michael/A-9805-2008; Epron, Daniel/B-5093-2011; Nouvellon, Yann/C-9295-2016 OI Ryan, Michael/0000-0002-2500-6738; Epron, Daniel/0000-0001-9451-3437; Nouvellon, Yann/0000-0003-1920-3847 NR 53 TC 16 Z9 16 U1 3 U2 54 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X J9 TREE PHYSIOL JI Tree Physiol. PD JUN PY 2012 VL 32 IS 6 SI SI BP 639 EP 643 DI 10.1093/treephys/tps055 PG 5 WC Forestry SC Forestry GA 962XY UT WOS:000305585000001 PM 22730478 ER PT J AU Domec, JC Ogee, J Noormets, A Jouangy, J Gavazzi, M Treasure, E Sun, G McNulty, SG King, JS AF Domec, Jean-Christophe Ogee, Jerome Noormets, Asko Jouangy, Julien Gavazzi, Michael Treasure, Emrys Sun, Ge McNulty, Steve G. King, John S. TI Interactive effects of nocturnal transpiration and climate change on the root hydraulic redistribution and carbon and water budgets of southern United States pine plantations SO TREE PHYSIOLOGY LA English DT Article DE carbon sequestration; Duke FACE; ecosystem respiration; hydraulic redistribution; loblolly pine (Pinus taeda L; ); MuSICA; soil water content; transpiration ID ELEVATED ATMOSPHERIC CO2; VAPOR-PRESSURE DEFICIT; CANOPY LEAF-AREA; LONG-TERM GROWTH; LOBLOLLY-PINE; SOIL-WATER; STOMATAL CONDUCTANCE; NIGHTTIME TRANSPIRATION; INTERSPECIFIC VARIATION; NUTRIENT AVAILABILITY AB Deep root water uptake and hydraulic redistribution (HR) have been shown to play a major role in forest ecosystems during drought, but little is known about the impact of climate change, fertilization and soil characteristics on HR and its consequences on water and carbon fluxes. Using data from three mid-rotation loblolly pine plantations, and simulations with the process-based model MuSICA, this study indicated that HR can mitigate the effects of soil drying and had important implications for carbon uptake potential and net ecosystem exchange (NEE), especially when N fertilization is considered. At the coastal site (C), characterized by deep organic soil, HR increased dry season tree transpiration (T) by up to 40%, and such an increase affected NEE through major changes in gross primary productivity (GPP). Deep-rooted trees did not necessarily translate into a large volume of HR unless soil texture allowed large water potential gradients to occur, as was the case at the sandy site (S). At the Piedmont site (P) characterized by a shallow clay-loam soil, HR was low but not negligible, representing up to 10% of T. In the absence of HR, it was predicted that at the C, S and P sites, annual GPP would have been diminished by 19, 7 and 9%, respectively. Under future climate conditions HR was predicted to be reduced by up to 25% at the C site, reducing the resilience of trees to precipitation deficits. The effect of HR on T and GPP was predicted to diminish under future conditions by 12 and 6% at the C and P sites, respectively. Under future conditions, T was predicted to stay the same at the P site, but to be marginally reduced at the C site and slightly increased at the S site. Future conditions and N fertilization would decrease T by 25% at the C site, by 15% at the P site and by 8% at the S site. At the C and S sites, GPP was estimated to increase by 18% and by > 70% under future conditions, respectively, with little effect of N fertilization. At the P site, future conditions would stimulate GPP by only 12%, but future conditions plus N fertilization would increase GPP by 24%. As a consequence, in all sites, water use efficiency was predicted to improve dramatically with future conditions. Modeling the effect of reduced annual precipitation indicated that limited water availability would decrease all carbon fluxes, including NEE and respiration. Our simulations highlight the interactive effects of nutrients and elevated CO2, and showed that the effect of N fertilization would be greater under future climate conditions. C1 [Domec, Jean-Christophe] Univ Bordeaux, Bordeaux Sci Agro, UMR TCEM ENITA INRA 1220, F-33175 Gradignan, France. [Domec, Jean-Christophe; Noormets, Asko; King, John S.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Ogee, Jerome; Jouangy, Julien] INRA, EPHYSE UR1263, F-33140 Villenave Dornon, France. [Gavazzi, Michael; Treasure, Emrys; Sun, Ge; McNulty, Steve G.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC 27606 USA. [King, John S.] Univ Antwerp, Dept Biol, B-2610 Antwerp, Belgium. RP Domec, JC (reprint author), Univ Bordeaux, Bordeaux Sci Agro, UMR TCEM ENITA INRA 1220, 1 Cours Gen Gaulle, F-33175 Gradignan, France. EM jdomec@ncsu.edu RI Ogee, Jerome/C-7185-2013; OI Noormets, Asko/0000-0003-2221-2111 FU USDA Forest Service Raleigh Eastern Forest Environmental Threat Assessment Center (EFETAC) [03-CA-11330147-073, 04-CA-11330147-238]; ANR project MIST [ANR-07-BLN-0131]; National Institute of Food and Agriculture (NIFA) [2011-67009-20089]; DOE National Institute of Climatic Change Research (NICCR) [08-SC-NICCR-1072]; DOE-BER [11-DE-SC-0006700, ER65189] FX This study was supported by the USDA Forest Service Raleigh Eastern Forest Environmental Threat Assessment Center (EFETAC) cooperative agreements 03-CA-11330147-073 and 04-CA-11330147-238. We thank the Weyerhaeuser Company for facilitating access to the coastal study site and offering logistical assistance. Salary for J.J. was supported through the ANR project MIST (ANR-07-BLN-0131) awarded to J.O. Sabbatical support for J.S.K. during the writing of this manuscript was provided by the University of Antwerp Class of Excellence program, the Belgian International Francqui Foundation, and the US Department of State WJ Fulbright Program. Additional support for research was provided by National Institute of Food and Agriculture (NIFA) Sustainable Bioenergy Program grant 2011-67009-20089, the DOE National Institute of Climatic Change Research (NICCR) grant 08-SC-NICCR-1072 and the DOE-BER Terrestrial Ecosystem Sciences program, proposal 11-DE-SC-0006700 - grant ER65189. NR 127 TC 32 Z9 34 U1 3 U2 82 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X EI 1758-4469 J9 TREE PHYSIOL JI Tree Physiol. PD JUN PY 2012 VL 32 IS 6 SI SI BP 707 EP 723 DI 10.1093/treephys/tps018 PG 17 WC Forestry SC Forestry GA 962XY UT WOS:000305585000007 PM 22467712 ER PT J AU Sala, A Woodruff, DR Meinzer, FC AF Sala, Anna Woodruff, David R. Meinzer, Frederick C. TI Carbon dynamics in trees: feast or famine? SO TREE PHYSIOLOGY LA English DT Review DE carbohydrates; carbon allocation; embolism; hydraulic safety; regulated storage; tree non-structural carbon storage ID LEAF HYDRAULIC CONDUCTANCE; DROUGHT-INDUCED MORTALITY; FREEZE-THAW CYCLES; WATER-STRESS; CARBOHYDRATE RESERVES; STOMATAL CONDUCTANCE; XYLEM EMBOLISM; TALL CONIFER; SCOTS PINE; FAGUS-SYLVATICA AB Research on the degree to which carbon (C) availability limits growth in trees, as well as recent trends in climate change and concurrent increases in drought-related tree mortality, have led to a renewed focus on the physiological mechanisms associated with tree growth responses to current and future climate. This has led to some dispute over the role of stored non-structural C compounds as indicators of a tree's current demands for photosynthate. Much of the uncertainty surrounding this issue could be resolved by developing a better understanding of the potential functions of non-structural C stored within trees. In addition to functioning as a buffer to reconcile temporal asynchrony between C demand and supply, the storage of non-structural C compounds may be under greater regulation than commonly recognized. We propose that in the face of environmental stochasticity, large, long-lived trees may require larger C investments in storage pools as safety margins than previously recognized, and that an important function of these pools may be to maintain hydraulic transport, particularly during episodes of severe stress. If so, survival and long-term growth in trees remain a function of C availability. Given that drought, freeze-thaw events and increasing tree height all impose additional constraints on vascular transport, the common trend of an increase in non-structural carbohydrate concentrations with tree size, drought or cold is consistent with our hypothesis. If the regulated maintenance of relatively large constitutive stored C pools in trees serves to maintain hydraulic integrity, then the minimum thresholds are expected to vary depending on the specific tissues, species, environment, growth form and habit. Much research is needed to elucidate the extent to which allocation of C to storage in trees is a passive vs. an active process, the specific functions of stored C pools, and the factors that drive active C allocation to storage. C1 [Sala, Anna] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Woodruff, David R.; Meinzer, Frederick C.] US Forest Serv, USDA, Forestry Sci Lab, Corvallis, OR 97331 USA. RP Sala, A (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. EM sala@mso.umt.edu RI Meinzer, Frederick/C-3496-2012 FU National Science Foundation (NSF) [DEB 05-15756] FX A.S. benefitted from funding from the National Science Foundation (NSF DEB 05-15756 to E. Crone and A. Sala) to develop the ideas in this paper. NR 92 TC 193 Z9 206 U1 13 U2 197 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0829-318X J9 TREE PHYSIOL JI Tree Physiol. PD JUN PY 2012 VL 32 IS 6 SI SI BP 764 EP 775 DI 10.1093/treephys/tpr143 PG 12 WC Forestry SC Forestry GA 962XY UT WOS:000305585000011 PM 22302370 ER PT J AU Corwin, DL AF Corwin, Dennis L. TI Field-scale monitoring of the long-term impact and sustainability of drainage water reuse on the west side of California's San Joaquin Valley SO JOURNAL OF ENVIRONMENTAL MONITORING LA English DT Article ID SOIL ELECTRICAL-CONDUCTIVITY; SALT-TOLERANT FORAGES; SPATIAL VARIABILITY; SALINE WATER; QUALITY; SYSTEMS; IRRIGATION; ADSORPTION; PREDICTION; MODEL AB Diminishing freshwater resources have brought attention to the reuse of degraded water as a water resource rather than a disposal problem. Drainage water from tile-drained, irrigated agricultural land is degraded water that is often in large supply, but the long-term impact and sustainability of its reuse on soil is unknown. Similarly, nothing is known of the ramifications of terminating drainage water reuse. The objective of this study is (i) to monitor the long-term impact on soil chemical properties and thereby the sustainability of drainage water reuse on a marginally productive, saline-sodic, 32.4 ha field located on the west side of California's productive San Joaquin Valley and (ii) to assess spatially what happens to soil when drainage water reuse is terminated. The monitoring and assessment were based on spatial chemical data for soil collected during 10 years of irrigation with drainage water followed by 2 years of no applied irrigation water (only rainfall). Geo-referenced measurements of apparent soil electrical conductivity (ECa) were used to direct the soil sampling design to characterize spatial variability of impacted soil properties. Chemical analyses of soil samples were used (i) to characterize the spatial variability of salinity, Na, B, and Mo, which were previously identified as critical to the yield and quality of Bermuda grass (Cynodon dactylon (1.) Pers.) grown for livestock consumption and (ii) to monitor their change during the 12 year study. Soil samples were taken at 0.3 m increments to a depth of 1.2 m at each of 40 sample sites on five occasions: August 1999, April 2002, November 2004, August 2009, and May 2011. Drainage water varying in salinity (1.8-16.3 dS m(-1)), SAR (5.2-52.4), Mo (80-400 mu g L-1), and B (0.4-15.1 mg L-1) was applied from July 2000 to June 2009. Results indicate that salts, Na, Mo, and B were leached from the root zone causing a significant improvement in soil quality from 1999 to 2009. Salinity and SAR returned to original levels or higher in less than two years after termination of irrigation. Boron and Mo showed significant increases. Long-term sustainability of drainage water reuse was supported by the results, but once application of irrigation water was terminated, the field quickly returned to its original saline-sodic condition. C1 USDA ARS, US Salin Lab, Riverside, CA 92507 USA. RP Corwin, DL (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Dennis.Corwin@ars.usda.gov FU University of California; University of California's Kearney Foundation FX The author acknowledges the initial collaboration of Dr Stephen Kaffka and Dr Jim Oster whose involvement was critical for the commencement of this project and without whom the project would not have been initiated. The author acknowledges the University of California Salinity-Drainage Program for the initial start-up funds used for field-site preparation and costs associated with initial sample collection and site management and the University of California's Kearney Foundation for funds to support the chemical analyses needed for the initial site characterization. Over the past 12 years a number of individuals have been involved with the field ECa surveys, field soil sampling, and laboratory analyses. The author thanks Kevin Yemoto and Clay Wilkinson for their technical support in the field and laboratory; Harry Forster and Nahid Visteh for their technical laboratory support; and Jack Jobes for his help in collecting a portion of the soil samples; as well as the following students for their assistance in soil sample preparation and routine physical and chemical soil analyses: Derrick Lai, Linda Lam, Jon Edwards, Jennifer Palarca, Samantha Chang, Janice Mandap, and Amanda Paul. The author is indebted to Ceil Howe, Jr and Ceil Howe III for the use of their land and for managing the dayto- day farm operations, which made the project possible. NR 35 TC 2 Z9 2 U1 2 U2 26 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 1464-0325 J9 J ENVIRON MONITOR JI J. Environ. Monit. PD JUN PY 2012 VL 14 IS 6 BP 1576 EP 1596 DI 10.1039/c2em10796a PG 21 WC Chemistry, Analytical; Environmental Sciences SC Chemistry; Environmental Sciences & Ecology GA 950PJ UT WOS:000304660900008 PM 22535499 ER PT J AU Lugo, AE Carlo, TA Wunderle, JM AF Lugo, A. E. Carlo, T. A. Wunderle, J. M., Jr. TI Natural mixing of species: novel plant-animal communities on Caribbean Islands SO ANIMAL CONSERVATION LA English DT Review DE novel communities; introduced species; Caribbean; Puerto Rico; US Virgin Islands; community assembly; naturalized species; species invasions; tropical forests; species eradication ID NORTHEASTERN PUERTO-RICO; POPULATION-DYNAMICS; SECONDARY FORESTS; HABITAT; LANDSCAPE; ECOLOGY; SINKS; LAND; CONSERVATION; PLANTATIONS AB Global anthropogenic activities are responsible for the modification of landscapes, creation of novel environments and movement of species across biogeographic regions. A consequence of this activity is the mixing of native and introduced species and the formation of novel biotic communities. We review the ecological consequences of the mixing of native and introduced species in the Caribbean Islands especially in Puerto Rico and the US Virgin Islands. Here we found documented examples indicating that novel communities of native and naturalized organisms are ubiquitous. The coexistence of species originating from different biogeographical regions raises research questions that demand attention for their ecological and conservation importance. For example: Is animal abundance in novel communities a measure of habitat quality? To what degree are populations in novel communities self-sustaining? What are the consequences of species eradication? How does an introduced animal's trophic position affect its effects on novel and native communities? We suggest that novel communities that emerge in the Caribbean after deforestation and land abandonment could be harbingers of how the biota might respond elsewhere to rapidly changing environmental conditions, including global and climate change. C1 [Lugo, A. E.; Wunderle, J. M., Jr.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR 00926 USA. [Carlo, T. A.] Penn State Univ, University Pk, PA 16802 USA. RP Lugo, AE (reprint author), US Forest Serv, Int Inst Trop Forestry, USDA, 1201 Calle Ceiba, Rio Piedras, PR 00926 USA. EM alugo@fs.fed.us NR 74 TC 16 Z9 17 U1 2 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1367-9430 J9 ANIM CONSERV JI Anim. Conserv. PD JUN PY 2012 VL 15 IS 3 BP 233 EP 241 DI 10.1111/j.1469-1795.2012.00523.x PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 956VW UT WOS:000305118700006 ER PT J AU Chapman, JW Dumbauld, BR Itani, G Markham, JC AF Chapman, John W. Dumbauld, Brett R. Itani, Gyo Markham, John C. TI An introduced Asian parasite threatens northeastern Pacific estuarine ecosystems SO BIOLOGICAL INVASIONS LA English DT Article DE Isopod; Parasite; Estuary; Extinction; Invasion; Orthione griffenis; Upogebia pugettensis ID SHRIMP UPOGEBIA-PUGETTENSIS; MUD SHRIMP; ORTHIONE-GRIFFENIS; RECRUITMENT LIMITATION; BOPYRIDAE CRUSTACEA; MARINE ORGANISMS; NORTH-AMERICA; TRANSPORT; DECAPODA; INVASION AB The introduced Asian parasitic bopyrid isopod, Orthione griffenis, was first discovered on the Pacific coast of North America in Washington in 1988 and next in California in 1992. The range of Orthione presently extends from British Columbia to Baja California, where it infests at least two species of the native estuary mud shrimp, Upogebia. Intense Orthione infestations are associated with the apparent demise of many local populations of Upogebia pugettensis yet nonindigenous origins of Orthione in North America and thus the ecological significance of its impacts have remained in doubt. Six criteria reveal that Orthione is introduced to North America: its conspecificity with disjunct Asian populations, its earliest (1950s) collections in Asia, its late discovery among symbiotic species associated with Upogebia, its historical absence, and its appearance in North America coincident with extensive new ballast water traffic from Asia. Orthione is the first recognized bopyrid isopod invasion globally. Coexistence of U. pugettensis, which are ecosystem engineers, with its newly acquired parasite cannot be assumed. Orthione threatens eastern Pacific estuary ecosystems where Upogebia were previously abundant. C1 [Chapman, John W.] Oregon State Univ, Hatfield Marine Sci Ctr, Newport, OR 97365 USA. [Dumbauld, Brett R.] ARS, USDA, Hatfield Marine Sci Ctr, Newport, OR 97365 USA. [Itani, Gyo] Kochi Univ, Fac Educ, Lab Marine Symbiot Biol, Kochi 7808520, Japan. [Markham, John C.] Arch Cape Marine Lab, Arch Cape, OR 97102 USA. RP Chapman, JW (reprint author), Oregon State Univ, Hatfield Marine Sci Ctr, Newport, OR 97365 USA. EM John.Chapman@OregonState.Edu OI Itani, Gyo/0000-0002-0437-2552 FU Washington State contingency funds for biological control of burrowing shrimp FX Washington State contingency funds for biological control of burrowing shrimp partially funded this research. James Carlton, Alan Shanks, and Kathy Hieb forwarded zooplankton samples and notes. Ardis Johnston (Harvard University Museum of Comparative Zoology), Bob Van Syoc (California Academy of Sciences), Eric Hochberg (Santa Barbara Museum of Natural History) and Leslie Harris and Regina Wetzer (Los Angeles County Museum of Natural History) assisted with their respective collections. We thank George Boehlert, Ryan Hechinger, Armand Kuris, Bill Peterson and Alan Shanks for insightful comments and advice. Mary Jo Adams, Ernesto Campos, Francis Choi, Andy Cohen, Anthony D'Andrea, Rusty Fairey, Tom Gaumer, Travis Goodman, Greg Jensen, Eugene Kozloff, Michael McGowan, Todd Newberry, John Pearse, Bruno Pernet, Martin Posey, Austin Pritchard, Jerry Rudy, Erik Thuesen, Cynthia Trowbridge, Kristen Wasson and Jason Williams provided pre- and post 1990 s data, specimens, or accounts of eastern Pacific Upogebia and Orthione populations. Susan Swarbrick, Jeff Crooks, Ralph Breitenstein, Eric Robbins, Carol Cole, Lee McCoy, and Cara Fritz helped with sampling, data and analyses. We thank Sylvia Yamada, Jennifer Ruesink and James Carlton for their critical reviews of the manuscript. NR 75 TC 10 Z9 14 U1 2 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 J9 BIOL INVASIONS JI Biol. Invasions PD JUN PY 2012 VL 14 IS 6 BP 1221 EP 1236 DI 10.1007/s10530-011-0151-3 PG 16 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 958ZB UT WOS:000305278400011 ER PT J AU Stephens, SL McIver, JD Boerner, REJ Fettig, CJ Fontaine, JB Hartsough, BR Kennedy, PL Schwilk, DW AF Stephens, Scott L. McIver, James D. Boerner, Ralph E. J. Fettig, Christopher J. Fontaine, Joseph B. Hartsough, Bruce R. Kennedy, Patricia L. Schwilk, Dylan W. TI The Effects of Forest Fuel-Reduction Treatments in the United States SO BIOSCIENCE LA English DT Article DE fire surrogates; wildfire; fire ecology; forest management; forest conservation ID FIRE SURROGATE TREATMENTS; PONDEROSA PINE FORESTS; MIXED-CONIFER FORESTS; SIERRA-NEVADA; PRESCRIBED-FIRE; TREE MORTALITY; VEGETATION STRUCTURE; NORTHERN CALIFORNIA; NATIONAL FIRE; SEVERITY AB The current conditions of many seasonally dry forests in the western and southern United States, especially those that once experienced low- to moderate-intensity fire regimes, leave them uncharacteristically susceptible to high-severity wildfire. Both prescribed fire and its mechanical surrogates are generally successful in meeting short-term fuel-reduction objectives such that treated stands are more resilient to high-intensity wildfire. Most available evidence suggests that these objectives are typically accomplished with few unintended consequences, since most ecosystem components (vegetation, soils, wildlife, bark beetles, carbon sequestration) exhibit very subtle effects or so measurable effects at all. Although mechanical treatments do not serve as complete surrogates for fire, their application can help mitigate costs and liability in some areas. Desired treatment effects on fire hazards are transient, which indicates that after fuel-reduction management start:;, managers need to be persistent with repeated treatment, especially in the faster-growing forests in the southern United States. C1 [Stephens, Scott L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [McIver, James D.] Oregon State Univ, Oregon Agr Res Ctr, Union, NJ USA. [Boerner, Ralph E. J.] Ohio State Univ, Dept Evolut Ecol & Organismal Biol, Columbus, OH 43210 USA. [Fettig, Christopher J.] US Forest Serv, USDA, Pacific SW Res Stn, Davis, CA USA. [Fontaine, Joseph B.] Murdoch Univ, Sch Environm Sci, Perth, WA, Australia. [Hartsough, Bruce R.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Kennedy, Patricia L.] Oregon State Univ, Dept Fisheries & Wildlife, Eastern Oregon Agr Res Ctr, Union, NJ USA. [Schwilk, Dylan W.] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. RP Stephens, SL (reprint author), Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM sstephens@berkeley.edu RI Kennedy, Patricia/I-4902-2015; Fontaine, Joseph/M-9186-2013 OI Kennedy, Patricia/0000-0002-2090-1821; Fontaine, Joseph/0000-0002-6515-7864 FU US Department of Agriculture-US Department of the Interior FX We are grateful to the numerous field crews, forest managers, forest operators, land owners, and scientists that contributed to this project. This work was partially funded by the US Department of Agriculture-US Department of the Interior Joint Fire Sciences Program. We appreciate the comments provided to us by three anonymous reviewers, which improved the manuscript. NR 68 TC 104 Z9 106 U1 7 U2 86 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD JUN PY 2012 VL 62 IS 6 BP 549 EP 560 DI 10.1525/bio.2012.62.6.6 PG 12 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 958TB UT WOS:000305262500006 ER PT J AU Bain, DJ Green, MB Campbell, JL Chamblee, JF Chaoka, S Fraterrigo, JM Kaushal, SS Martin, SL Jordan, TE Parolari, AJ Sobczak, WV Weller, DE Wollheim, WM Boose, ER Duncan, JM Gettel, GM Hall, BR Kumar, P Thompson, JR Vose, JM Elliott, EM Leigh, DS AF Bain, Daniel J. Green, Mark B. Campbell, John L. Chamblee, John F. Chaoka, Sayo Fraterrigo, Jennifer M. Kaushal, Sujay S. Martin, Sherry L. Jordan, Thomas E. Parolari, Anthony J. Sobczak, William V. Weller, Donald E. Wollheim, Wilfred M. Boose, Emery R. Duncan, Jonathan M. Gettel, Gretchen M. Hall, Brian R. Kumar, Praveen Thompson, Jonathan R. Vose, James M. Elliott, Emily M. Leigh, David S. TI Legacy Effects in Material Flux: Structural Catchment Changes Predate Long-Term Studies SO BIOSCIENCE LA English DT Article DE legacy effect; long-term studies; structural and signal legacy effects; sedimentation ID RHODE RIVER WATERSHEDS; NORTHERN HARDWOOD FOREST; LAND-USE CHANGE; AGRICULTURAL ABANDONMENT; AIR-TEMPERATURE; SOIL-NITROGEN; FRESH-WATER; CARBON; PRECIPITATION; DISCHARGES AB Legacy effects of past land use and disturbance are increasingly recognized, yet consistent definitions of and criteria for defining them do not exist. To address this gap in biological- and ecosystem-assessment frameworks, we propose a general metric for evaluating potential legacy effects, which are computed by normalizing altered system function persistence with duration of disturbance. We also propose two distinct legacy-effect categories: signal effects from lags in transport and structural effects from physical landscape changes. Using flux records for water, sediment, nitrogen, and carbon from long-term study sites in the eastern United States from 1500 to 2000, we identify gaps in our understanding of legacy effects and reveal that changes in basin sediment dynamics precede instrumented records. These sediment dynamics are not generally incorporated into interpretations of contemporary records, although their potential legacy effects are substantial. The identification of legacy effects may prove to be a fundamental component of landscape management and effective conservation and restoration practice. C1 [Bain, Daniel J.; Elliott, Emily M.] Univ Pittsburgh, Dept Geol & Planetary Sci, Pittsburgh, PA 15260 USA. [Green, Mark B.] Plymouth State Univ, Ctr Environm, Plymouth, NH USA. [Green, Mark B.; Campbell, John L.] US Forest Serv, No Res Stn, Durham, NH USA. [Chaoka, Sayo; Kumar, Praveen] Univ Illinois, Dept Civil & Environm Engn, Urbana, IL 61801 USA. [Fraterrigo, Jennifer M.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Kaushal, Sujay S.] Univ Maryland, Dept Geol, College Pk, MD 20742 USA. [Kaushal, Sujay S.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. [Martin, Sherry L.] Michigan State Univ, Dept Geol Sci, E Lansing, MI 48824 USA. [Jordan, Thomas E.; Weller, Donald E.] Smithsonian Environm Res Ctr, Edgewater, MD 21037 USA. [Parolari, Anthony J.] MIT, Dept Civil & Environm Engn, Cambridge, MA 02139 USA. [Sobczak, William V.] Coll Holy Cross, Dept Biol, Worcester, MA 01610 USA. [Wollheim, Wilfred M.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Wollheim, Wilfred M.] Univ New Hampshire, Environm & Complex Syst Res Ctr, Durham, NH 03824 USA. [Boose, Emery R.; Hall, Brian R.] Harvard Univ Harvard Forest, Petersham, MA USA. [Duncan, Jonathan M.] Univ N Carolina, Dept Geog, Chapel Hill, NC USA. [Gettel, Gretchen M.] United Nations Educ Sci & Cultural Org, Int Inst Infrastruct Hydraul & Environm Engn, Delft, Netherlands. [Thompson, Jonathan R.] Smithsonian Inst, Smithsonian Conservat Biol Inst, Front Royal, VA USA. [Vose, James M.] US Forest Serv, So Res Stn, Otto, NC USA. [Leigh, David S.] Univ Georgia, Dept Geog, Athens, GA 30602 USA. RP Bain, DJ (reprint author), Univ Pittsburgh, Dept Geol & Planetary Sci, Pittsburgh, PA 15260 USA. EM dbain@pitt.edu RI Kumar, Praveen/D-2036-2010; Chamblee, John/A-7168-2013; Elliott, Emily /E-8122-2011; Bain, Daniel/G-3055-2013; Parolari, Anthony/I-5137-2013; Gettel, Gretchen/M-8983-2013; Kaushal, Sujay/G-1062-2013; OI Kumar, Praveen/0000-0002-4787-0308; Green, Mark/0000-0002-7415-7209; Campbell, John/0000-0003-4956-1696; Chamblee, John/0000-0002-7776-0302; Elliott, Emily /0000-0002-9653-1513; Gettel, Gretchen/0000-0002-9288-1583; Kaushal, Sujay/0000-0003-0834-9189; Weller, Donald/0000-0002-7629-5437 FU Long Term Ecological Research Network Office (National Science Foundation [NSF]) [DEB-0236154]; NSF Division of Earth Sciences [0854957]; NSF's Long-Term Ecological Research Network [DEB-1027188, DEB-0823293, DEB-1114804, DEB-0620443, NSF-OCE-0423565, NSF-OCE-1058747]; NSF [DEB-79-11563, DEB-82072-12, CEE-8219615, BSR-8316948, BSR-8615902, BSR-8905219, BSR-891703, BSR-9085219, DEB-92-06811, DEB-93-17968]; National Oceanic and Atmospheric Administration [NA66RG0129]; US Environmental Protection Agency [804536] FX This manuscript grew out of a working group sponsored by the Long Term Ecological Research Network Office (National Science Foundation [NSF] Cooperative Agreement no. DEB-0236154), with supplementary funding from NSF Division of Earth Sciences grant no. 0854957 to the City University of New York. This material is based on work at Long Term Ecological Research sites supported by the NSF's Long-Term Ecological Research Network under the following grant numbers: DEB-1027188 to the Baltimore Ecosystem Study, DEB-0823293 to the Coweeta Hydrologic Laboratory, DEB-1114804 to the Hubbard Brook Experimental Forest, DEB-0620443 to the Harvard Forest site, and NSF-OCE-0423565 and NSF-OCE-1058747 to the Plum Island Ecosystem. The Smithsonian Environmental Research Center watershed discharge data were collected with support from the NSF (grant nos. DEB-79-11563, DEB-82072-12, CEE-8219615, BSR-8316948, BSR-8615902, BSR-8905219, BSR-891703, BSR-9085219, DEB-92-06811, and DEB-93-17968), from the National Oceanic and Atmospheric Administration Coastal Oceans Program (grant no. NA66RG0129), the US Environmental Protection Agency (grant no. 804536), and the Smithsonian Institution Environmental Sciences Program. The careful comments of three anonymous reviewers improved the article. NR 57 TC 26 Z9 28 U1 2 U2 59 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD JUN PY 2012 VL 62 IS 6 BP 575 EP 584 DI 10.1525/bio.2012.62.6.8 PG 10 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 958TB UT WOS:000305262500008 ER PT J AU Li, WL Li, XH Fan, X Tang, Y Yun, J AF Li, W. L. Li, X. H. Fan, X. Tang, Y. Yun, J. TI Response of antioxidant activity and sensory quality in fresh-cut pear as affected by high O-2 active packaging in comparison with low O-2 packaging SO FOOD SCIENCE AND TECHNOLOGY INTERNATIONAL LA English DT Article DE Fresh-cut pears; high O-2 active packaging; antioxidant capacity; sensory quality ID MODIFIED ATMOSPHERE; POSTHARVEST PHYSIOLOGY; HIGH-OXYGEN; VITAMIN-C; FRUITS; VEGETABLES; STORAGE; RESPIRATION; METABOLISM; EXTRACTS AB Effects of active modified atmosphere packaging (initial O-2/CO2: 5/5; 30/5; and 80/0) and passive packaging [initial O-2/CO2: 20.8/0 (air)] on the antioxidant capacity and sensory quality of fresh-cut 'Yaoshan' pear stored at 4 degrees C for 12 days were investigated. Samples stored in high O-2 (30% and 80%) packages had higher phenolics and anthocyanin contents compared with those in passive and low O-2 packages. After 12 days of storage, phenolics and anthocyanin contents of 80% O-2 samples were 2.5 and 12 times, respectively, higher than those in the passive package and 3 and 2 times higher than those in low O-2 package. High O-2 modified atmosphere packaging was effective in keeping free radical scavenging capacity as measured by the DPPH assay. The sensory evaluation indicated that surface color of cut fruits were stable for at least 12 days in the high O-2 modified atmosphere packaging. The results suggested that high O-2 modified atmosphere packaging could be used to inhibit browning and prolong the shelf life of fresh-cut 'Yaoshan' pears in spite of more than 50% loss in vitamin C content. C1 [Li, W. L.; Li, X. H.; Tang, Y.; Yun, J.] Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ, Tianjin 300457, Peoples R China. [Fan, X.] ARS, USDA, Eastern Reg Res Ctr, Sacramento, CA USA. RP Li, XH (reprint author), Tianjin Univ Sci & Technol, Key Lab Food Nutr & Safety, Minist Educ, Tianjin 300457, Peoples R China. EM liweili1207@yahoo.cn RI Yun, Juan/E-4164-2016 FU National Natural Science Fund [31000826]; National High-New Technology Research Development Plan-863 FX This study is supposed by "National Natural Science Fund 31000826" and "National High-New Technology Research Development Plan-863". NR 35 TC 1 Z9 1 U1 3 U2 23 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 1082-0132 J9 FOOD SCI TECHNOL INT JI Food Sci. Technol. Int. PD JUN PY 2012 VL 18 IS 3 BP 197 EP 205 DI 10.1177/1082013211415147 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 958AL UT WOS:000305208200001 PM 22397831 ER PT J AU McRoberts, RE Tomppo, EO Schadauer, K Stahl, G AF McRoberts, Ronald E. Tomppo, Erkki O. Schadauer, Klemens Stahl, Goran TI Harmonizing National Forest Inventories SO FOREST SCIENCE LA English DT Editorial Material C1 [McRoberts, Ronald E.] US Forest Serv, N Cent Forest Res Stn, Forest Inventory & Anal, St Paul, MN 55108 USA. [Stahl, Goran] Swedish Univ Agr Sci, S-75007 Uppsala, Sweden. RP McRoberts, RE (reprint author), US Forest Serv, N Cent Forest Res Stn, Forest Inventory & Anal, 1992 Folwell Ave, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us; erkki.tomppo@metla.fi; klemens.schadauer@bfw.gv.at; goran.stahl@slu.se NR 12 TC 6 Z9 6 U1 0 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 189 EP 190 DI 10.5849/forsci.12-042 PG 2 WC Forestry SC Forestry GA 959KE UT WOS:000305310500001 ER PT J AU Gabler, K Schadauer, K Tomppo, E Vidal, C Bonhomme, C McRoberts, RE Gschwantner, T AF Gabler, Karl Schadauer, Klemens Tomppo, Erkki Vidal, Claude Bonhomme, Camille McRoberts, Ronald E. Gschwantner, Thomas TI An Enquiry on Forest Areas Reported to the Global Forest Resources Assessment-Is Harmonization Needed? SO FOREST SCIENCE LA English DT Article DE Global Forest Resources Assessment 2005; National Forest Inventories; COST Action E43 AB For international reporting purposes, information on forest resources often has to be supplied according to international definitions. Nevertheless, the country reports of the Food and Agriculture Organization (FAO) Global Forest Resources Assessment 2005 indicate that countries either prefer to use their own forest definitions or use national classes of forest and other biomes as a basis for reclassification to resolve the discrepancy between national and international forest definitions. These preferences are due to the complexity encountered when dealing with the problem of transforming national estimates into estimates according to an international definition but are also due to the lack of a methodological foundation. In view of the multitude of existing national forest definitions and the increasing international reporting requirements, a thorough methodological consideration of the problem appears to be particularly necessary. Therefore, a mathematical approach for comparing national definitions to an international definition is developed. After formalization of definitions and illustration of the peculiarities in national forest definitions, the basics of set theory and simple logic are applied to derive six relevant cases that describe how national definitions of forest are distinct from that of FAO. As a result, we are able to assign each country to a specific case and thus provide a basis for further analyses. We also reflect on the reclassification problem and describe two examples that illustrate the influence of assessment methods in the aim to report according to a common definition. FOR. SEI. 58(3):201-213. C1 [Gabler, Karl; Schadauer, Klemens; Gschwantner, Thomas] Fed Res & Training Ctr Forests Nat Hazards & Land, A-1131 Vienna, Austria. [Tomppo, Erkki] Finnish Forest Res Inst, Vantaa, Finland. [Vidal, Claude; Bonhomme, Camille] Inventaire Forestier Natl, Nogent Sur Vernisson, France. [McRoberts, Ronald E.] US Forest Serv, St Paul, MN 55108 USA. EM klemens.schadauer@bfw.gv.at; erkki.tomppo@metla.fi; claude.vidal@ifn.fr; camille.bonhomme@ifn.fr; rmcroberts@fs.fed.us; thomas.gschwantner@bfw.gy.at FU COST secretariat FX We are grateful for the financial support of the COST secretariat and especially to Gunter Siegel, and his successor, Melae Langbein, the officers responsible for Cost Action E43. We also highly appreciate the helpful comments of the two anonymous reviewers for improvement of the article. NR 40 TC 5 Z9 5 U1 1 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 201 EP 213 DI 10.5849/forsci.10-060 PG 13 WC Forestry SC Forestry GA 959KE UT WOS:000305310500003 ER PT J AU Stahl, G Cienciala, E Chirici, G Lanz, A Vidal, C Winter, S McRoberts, RE Rondeux, J Schadauer, K Tomppo, E AF Stahl, Goran Cienciala, Emil Chirici, Gherardo Lanz, Adrian Vidal, Claude Winter, Susanne McRoberts, Ronald E. Rondeux, Jacques Schadauer, Klemens Tomppo, Erkki TI Bridging National and Reference Definitions for Harmonizing Forest Statistics SO FOREST SCIENCE LA English DT Article DE forest resource assessment; statistics; harmonization; national forest inventory; standardization ID INVENTORY; SCALE AB Harmonization is the process of making information and estimates comparable across administrative borders. The degree to which harmonization succeeds depends on many factors, including the conciseness of the definitions, the availability and quality of data, and the methods used to convert an estimate according to a local definition to an estimate according to the reference definition. Harmonization requires the availability and use of common reference definitions and methods for converting from estimates based on national definitions to estimates based on reference definitions. This article focuses on conversion methods, which are characterized as "bridges" because they can be seen as a means of crossing from islands of local definitions to the mainland of a reference definition. A structured approach is proposed for constructing bridges of three kinds: reductive, neutral, and expansive bridges. A hierarchical decision tree is presented to guide users and to summarize the propositions and case examples with different types of bridges to illustrate the concepts. Although the article addresses harmonization of forest information, the results are relevant for harmonizing a broad variety of area statistics. FOR. SCI. 58(3):214-223. C1 [Stahl, Goran] Swedish Univ Agr Sci, Dept Forest Resource Management, S-90183 Umea, Sweden. [McRoberts, Ronald E.] US Forest Serv, NRS, Washington, DC USA. [Rondeux, Jacques] Univ Liege, B-4000 Liege, Belgium. [Chirici, Gherardo] Univ Molise, Campobasso, Italy. [Winter, Susanne] Tech Univ Munich, Munich, Germany. RP Stahl, G (reprint author), Swedish Univ Agr Sci, Dept Forest Resource Management, S-90183 Umea, Sweden. EM goran.stahl@slu.se; emil.cienciala@ifer.cz; gherardo.chirici@unimol.it; adrian.lantz@wsl.ch; claude.vidal@ifn.fr; winter@wzw.tum.de; rmcroberts@fs.fed.us; jrondeux@ulg.ac.be; klemens.schadauer@bfw.gv.at; erkki.tomppo@metla.fi RI Chirici, Gherardo/E-6364-2010; Cienciala, Emil/F-1873-2011 OI Chirici, Gherardo/0000-0002-0669-5726; Cienciala, Emil/0000-0002-1254-4254 NR 38 TC 19 Z9 21 U1 0 U2 11 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 214 EP 223 DI 10.5849/forsci.10-067 PG 10 WC Forestry SC Forestry GA 959KE UT WOS:000305310500004 ER PT J AU Heikkinen, J Tomppo, E Freudenschuss, A Weiss, P Hylen, G Kusar, G McRoberts, R Kandler, G Cienciala, E Petersson, H Stahl, G AF Heikkinen, Juha Tomppo, Erkki Freudenschuss, Alexandra Weiss, Peter Hylen, Gro Kusar, Gal McRoberts, Ronald Kaendler, Gerald Cienciala, Emil Petersson, Hans Stahl, Goran TI Interpolating and Extrapolating Information from Periodic Forest Surveys for Annual Greenhouse Gas Reporting SO FOREST SCIENCE LA English DT Article DE climate change; Kyoto Protocol; tree biomass; national forest inventories AB National forest inventories (NFIs) are an important source of data for reporting greenhouse gas emissions and removals for the Land Use, Land-Use Change, and Forestry sector as required by the United Nations Framework Convention on Climate Change and its Kyoto Protocol. A major limitation is that NFI resources are generally not sufficient for producing reliable information on year-to-year variation. Interpolation, extrapolation, smoothing, and/or aggregation of data from several years are therefore needed to comply with the reporting requirements for a specific year. Various methods for accomplishing this task are illustrated and evaluated based on data and experiences from the NFIs of six countries, concentrating on the estimation of the stem volume of living trees as a surrogate for tree biomass. Six main conclusions were drawn: (1) NFI data from the target years only were not sufficient for reliable estimation of annual stock change; (2) changes between whole inventory cycles (typically 5 years) could be estimated with reasonable precision; (3) simple moving average estimators of stock are problematic in the estimation of changes; (4) interpenetrating panel designs with permanent sample plots are desirable from the point of view of inter/extrapolating and change estimation; (5) data on annual growth variation and harvests are important and can be used directly in the default method, which is based on differences between increment and drain; and (6) time gaps between NFI surveys may lead to significant errors in the estimation of stock changes. FOR. SCI. 58(3):236-247. C1 [Heikkinen, Juha; Tomppo, Erkki] Finnish Forest Res Inst, FI-01301 Vantaa, Finland. [Hylen, Gro] Norwegian Forest & Landscape Inst, As, Norway. [McRoberts, Ronald] US Forest Serv, No Res Stn, Washington, DC USA. [Kaendler, Gerald] Forest Res Inst Baden Wurttemberg, Baden Wurttemberg, Germany. [Petersson, Hans; Stahl, Goran] Swedish Univ Agr Sci, S-75007 Uppsala, Sweden. RP Heikkinen, J (reprint author), Finnish Forest Res Inst, POB 18, FI-01301 Vantaa, Finland. EM juha.heikkinen@metla.fi; erkki.tomppo@metla.fi; alexandra.freudenschuss@umweltbundesamt.at; peter.weiss@umweltbundesamt.at; gro.hylen@skogoglandskap.no; gal.kusar@guest.arnes.si; rmcroberts@fs.fed.us; gerald.kaendler@forst.bwl.de; emil.cienciala@ifer.cz; hans.petersson@slu.se; goran.stahl@slu.se RI Cienciala, Emil/F-1873-2011; Hylen, Gro/C-5754-2014 OI Cienciala, Emil/0000-0002-1254-4254; NR 22 TC 5 Z9 5 U1 1 U2 5 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 236 EP 247 DI 10.5849/forsci.10-086 PG 12 WC Forestry SC Forestry GA 959KE UT WOS:000305310500006 ER PT J AU Chirici, G McRoberts, RE Winter, S Bertini, R Brandli, UB Asensio, IA Bastrup-Birk, A Rondeux, J Barsoum, N Marchetti, M AF Chirici, Gherardo McRoberts, Ronald E. Winter, Susanne Bertini, Roberta Braendli, Urs-Beat Asensio, Iciar Alberdi Bastrup-Birk, Annemarie Rondeux, Jacques Barsoum, Nadia Marchetti, Marco TI National Forest Inventory Contributions to Forest Biodiversity Monitoring SO FOREST SCIENCE LA English DT Article DE COST Action E43; international references; harmonization; biodiversity indicator; naturalness; forest type; deadwood; forest age; regeneration ID COARSE WOODY DEBRIS; PACIFIC-NORTHWEST; STAND STRUCTURE; BEECH FORESTS; INDICATORS; CONSERVATION; MANAGEMENT; FRAMEWORK; USA; ASSOCIATIONS AB Forests are the most biodiverse terrestrial ecosystems. National forest inventories (NFIs) are the main source of information on the status and trends of forests, but they have traditionally been designed to assess land coverage and the production value of forests rather than forest biodiversity. The primary international processes dealing with biodiversity and sustainable forest management, the Convention on Biological Diversity (CBD), Forest Europe, Streamlining European Biodiversity Indicators 2010 of the European Environmental Agency, and the Montreal Process, all include indicators related to forest biodiversity. The scope of this article is to review and present possibilities offered by NFIs to harmonize estimation of indicators useful for international forest biodiversity monitoring and reporting. We summarize key findings from Working Group 3 of Action E43 ("Harmonisation of National Forest Inventories in Europe: Techniques for Common Reporting") of the European program Cooperation in Science and Technology (COST). We discuss definitions and techniques for harmonizing estimates of possible biodiversity indicators based on data from NFIs in Europe and the United States. We compare these possible indicators with indicators selected by international processes. The results demonstrate that NFIs can report comparable or harmonized estimates of indicators for multiple biodiversity features (forest categories, deadwood, forest age, forest structure, and forest naturalness), but for others (ground vegetation and regeneration) NFIs should invest more in harmonization efforts. On the basis of these key findings, we recommend that NFIs should represent a main component of a future global biodiversity monitoring network as urgently requested by the CBD. FOR. SCI. 58(3):257-268. C1 [Chirici, Gherardo; Marchetti, Marco] Univ Molise, I-86090 Pesche, Isernia, Italy. [Winter, Susanne] Tech Univ Dresden, Dresden, Germany. [McRoberts, Ronald E.] US Forest Serv, Washington, DC USA. [Bertini, Roberta] Univ Florence, I-50121 Florence, Italy. [Bastrup-Birk, Annemarie] Univ Copenhagen, DK-1168 Copenhagen, Denmark. [Rondeux, Jacques] Univ Liege, Gembloux Agrobiotech, B-4000 Liege, Belgium. RP Chirici, G (reprint author), Univ Molise, I-86090 Pesche, Isernia, Italy. EM gherardo.chirici@unimol.it RI Chirici, Gherardo/E-6364-2010; Marchetti, Marco/D-9277-2012; OI Chirici, Gherardo/0000-0002-0669-5726; Marchetti, Marco/0000-0002-5275-5769 NR 83 TC 20 Z9 20 U1 4 U2 40 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 257 EP 268 DI 10.5849/forsci.12-003 PG 12 WC Forestry SC Forestry GA 959KE UT WOS:000305310500008 ER PT J AU Rondeux, J Bertini, R Bastrup-Birk, A Corona, P Latte, N McRoberts, RE Stahl, G Winter, S Chirici, G AF Rondeux, Jacques Bertini, Roberta Bastrup-Birk, Annemarie Corona, Piermaria Latte, Nicolas McRoberts, Ronald E. Stahl, Goran Winter, Susanne Chirici, Gherardo TI Assessing Deadwood Using Harmonized National Forest Inventory Data SO FOREST SCIENCE LA English DT Article DE reference definitions; bridging functions; deadwood attributes; biodiversity indicator; carbon pool ID COARSE WOODY DEBRIS; BIODIVERSITY ASSESSMENTS; BEECH FORESTS; EUROPE; PERSPECTIVE; INDICATORS; MANAGEMENT; DIVERSITY; FUNGI; PLOTS AB Deadwood plays an important role in forest ecological processes and is fundamental for the maintenance of biological diversity. Further, it is a forest carbon pool whose assessment must be reported for international agreements dealing with protection and forest management sustainability. Despite wide agreement on deadwood monitoring by national forest inventories (NFIs), much work is still necessary to clarify definitions so that estimates can be directly compared or aggregated for international reporting. There is an urgent need for an international consensus on definitions and agreement on harmonization methods. The study addresses two main objectives: to analyze the feasibility of harmonization procedures for deadwood estimates and to evaluate the impact of the harmonization process based on different definitions on final deadwood estimates. Results are reported for an experimental harmonization test using NFI deadwood data from 9,208 sample plots measured in nine European countries and the United States. Harmonization methods were investigated for volume by spatial position (lying or standing), decay classes, and woody species accompanied by accuracy assessments. Estimates of mean plot volume based on harmonized definitions with minimum length/height of 1 m and minimum diameter thresholds of 10, 12, and 20 cm were on average 3, 8, and 30% smaller, respectively, than estimates based on national definitions. Volume differences were less when estimated for various deadwood categories. An accuracy assessment demonstrated that, on average, the harmonization procedures did not substantially alter deadwood observations (root mean square error 23.17%). FOR. SCI. 58(3):269-283. C1 [Rondeux, Jacques; Latte, Nicolas] Univ Liege, Gembloux Agrobio Tech, Unit Forest & Nat Management, B-5030 Namur, Belgium. [Bertini, Roberta] Univ Florence, I-50121 Florence, Italy. [Bastrup-Birk, Annemarie] Univ Copenhagen, DK-1168 Copenhagen, Denmark. [Corona, Piermaria] Univ Tuscia, Viterbo, Italy. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, Washington, DC USA. [Stahl, Goran] Swedish Univ Agr Sci, S-75007 Uppsala, Sweden. [Winter, Susanne] Tech Univ Munich, D-8000 Munich, Germany. [Chirici, Gherardo] Univ Molise, Campobasso, Italy. RP Rondeux, J (reprint author), Univ Liege, Gembloux Agrobio Tech, Unit Forest & Nat Management, Passage Deportes 2, B-5030 Namur, Belgium. EM jrondeux@ulg.ac.be; bertini.roberta@gmail.com; ab@life.ku.dk; piermaria.corona@unitus.it; nicolas.latte@ulg.ac.be; rmcroberts@fs.fed.us; goran.stahl@srh.slu.se; winter@wzw.tum.de; gherardo.chirici@unimol.it RI Chirici, Gherardo/E-6364-2010; Corona, Piermaria/G-1565-2011 OI Chirici, Gherardo/0000-0002-0669-5726; Corona, Piermaria/0000-0002-8105-0792 FU Italian Ministry of Research and University FX This article is based on the activities carried out in WG3 of COST Action E43 "Harmonisation of National Forest Inventories: Techniques for Common Reporting." We express our thanks to the chair of COST Action E43, Professor Erkki Tomppo, to the members of the steering committee (Klemens Schadauer, Emil Cienciala, Adrian Lanz, and Claude Vidal) and to those who were involved in the activities of WG3: Iciar Alberdi Asensio, Anna-Lena Axelsson, Anna Barbati, Nadia Barsoum, Jana Beranova, Urs-Beat Brandli, Vladimir Caboun, Catherine Cluzeau, Sebastiano Cullotta, Leandras Deltuva, Paulo Godinho-Ferreira, Nabila Hamza, Elmar Hauk, Albertas Kasperavicius, Marko Kovac, Marco Marchetti, Helena Make la, Stefan Neagu, Jan-Erik Nilsen, Dieter Pelz, Tarmo Tolm, Tiina Tonteri, and Theocharis Zagas. For contributing deadwood data for the common DB, we gratefully acknowledge the following: Belgium: Jacques Rondeux and Nicolas Latte, Unit of Forest and Nature Management, University of Liege (Gembloux Agro-Bio Tech); Switzerland: Urs-Beat Brandli and Ulrich Ulmer, Snow and Landscape Research (WSL), Swiss Federal Institute for Forest; Germany: Heino Polley, Johann Heinrich von Thunen-Institut, Eberswalde; Denmark: Annemarie Bastrup-Birk and Thomas Nord-Larsen, Forest and Landscape, University of Copenhagen; Spain: Roberto Vallejo, Ministerio de Medio Ambiente y Medio Rural y Marino, and Iciar Alberdi Asensio and Sonia Condes, E.T.S.I. Montes de Madrid; Finland: Helena Make la, Finnish Forest Research Institute, Met la; Italy: Marco Marchetti, University of Molise; Sweden: Jonas Fridman and Anna-Lena Axelsson, Swedish University of Agricultural Sciences; United States of America: Forest Inventory and Analysis program of the Northern Research Station, US Forest Service. We acknowledge Alessandro Mastronardi (www.gm-studio.net) for the graphical three-dimensional rendering in Figure 2. We thank the associate editor of Forest Science and two anonymous reviewers who help us in improving the quality of an earlier draft of this article. This study was partially supported by the Italian Ministry of Research and University within the PRIN 2007 project "Innovative methods for the identification, characterization and management of old-growth forests in the Mediterranean environment." NR 47 TC 10 Z9 10 U1 1 U2 19 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 269 EP 283 DI 10.5849/forsci.10-057 PG 15 WC Forestry SC Forestry GA 959KE UT WOS:000305310500009 ER PT J AU Winter, S Bock, A McRoberts, RE AF Winter, Susanne Boeck, Andreas McRoberts, Ronald E. TI Uncertainty of Large-Area Estimates of Indicators of Forest Structural Gamma Diversity: A Study Based on National Forest Inventory Data SO FOREST SCIENCE LA English DT Article DE national forest inventory; international reporting on biodiversity; tree diameter and height; spatial autocorrelation ID BIODIVERSITY INDICATORS; DIAMETER DISTRIBUTIONS; STAND STRUCTURE; BOREAL FORESTS; BEECH FORESTS; CONSERVATION; ABUNDANCE; RICHNESS; NORWAY; TRENDS AB Tree diameter and height are commonly measured forest structural variables, and indicators based on them are candidates for assessing forest diversity. We conducted our study on the uncertainty of estimates for mostly large geographic scales for four indicators of forest structural gamma diversity: mean tree diameter, mean tree height, and standard deviations of tree diameter and tree height. We had three objectives: to estimate the number of national forest inventory (NFI) plots whose data must be aggregated to obtain precise estimates of the indicators of gamma forest structural diversity; to assess differences in structural associations with respect to different forest categories; and to assess the effects of geographic distance on estimates of indicators of forest structural diversity. Uncertainty assessments were conducted using a bootstrap resampling approach and a geospatial approach to assess the effects of geographic distances. Our study relied on a database populated with NFI plot data (12,536 plots and 256,513 trees) from 13 European countries and three ecoprovinces of the United States. The results were threefold. First, data from approximately 150 NFI plots randomly selected from a large database are generally sufficient to estimate standard deviations (SDs) of diameter and height as indicators of structural forest diversity at large scales with acceptable precision. Second, the uncertainties of the estimates were generally greater for forest categories in Europe than in the United States with the exception of Douglas fir forests in the Pacific Northwest of the United States. Third, diameter and height gamma diversities expressed by the means and SDs of distributions of tree diameter and tree height were more similar for geographic areas separated by smaller distances. FOR. SCI. 58(3):284-293. C1 [Winter, Susanne] Tech Univ Dresden, Inst Gen Ecol & Environm Protect, D-01737 Tharandt, Germany. [Boeck, Andreas] Tech Univ Munich, D-8000 Munich, Germany. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, Washington, DC USA. RP Winter, S (reprint author), Tech Univ Dresden, Inst Gen Ecol & Environm Protect, Pienner Str 7, D-01737 Tharandt, Germany. EM susanne.winter@forst.tu-dresden.de; andreas.boeck@tum.de; rmcroberts@fs.fed.us NR 62 TC 3 Z9 3 U1 0 U2 10 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 284 EP 293 DI 10.5849/forsci.10-076 PG 10 WC Forestry SC Forestry GA 959KE UT WOS:000305310500010 ER PT J AU McRoberts, RE Winter, S Chirici, G LaPoint, E AF McRoberts, Ronald E. Winter, Susanne Chirici, Gherardo LaPoint, Elizabeth TI Assessing Forest Naturalness SO FOREST SCIENCE LA English DT Article DE harmonization; biodiversity indicators; hemeroby ID TREE-SIZE DIVERSITY; FAGUS-SYLVATICA; BIOLOGICAL DIVERSITY; STRUCTURAL DIVERSITY; STAND STRUCTURE; BEECH FORESTS; DEAD WOOD; DIAMETER DISTRIBUTION; CONSERVATION BIOLOGY; SPRUCE FORESTS AB The concept of naturalness has been proposed and used for describing the ecological value of forest ecosystems, evaluating management efforts to conserve biodiversity, and identifying natural, old-growth forests for purposes of establishing protection areas. Because the concept is not globally familiar, a literature review was conducted to document the ecological basis for the concept. In addition, the necessity for harmonized reporting motivated an investigation of variables that can be used to quantify and assess forest naturalness. National forest inventories (NFIs) are sources of the most comprehensive and extensive data available for assessing naturalness. However, the variety of NFI plot configurations, sampling designs, definitions, and measurement protocols greatly impedes the utility of NFI data for purposes of producing compatible estimates. As a component of a pan-European harmonization project, a three-phase investigation of harmonized approaches to assessing forest naturalness using NFI variables was conducted. Although the project was primarily European in scope, forest inventory data for an American ecoprovince were used because of their availability, comprehensiveness, and consistency. The primary result is that NFT features such as minimum dbh and plot size affect estimates of indicators of naturalness, and accommodation of their differences may be necessary if harmonized estimates of indicators of naturalness across countries are required. FOR. SCI. 58(3):294-309. C1 [McRoberts, Ronald E.; LaPoint, Elizabeth] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Winter, Susanne] Tech Univ Dresden, Dresden, Germany. [Chirici, Gherardo] Univ Molise, Campobasso, Italy. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us; Susanne.Winter@forst.tu-dresden.de; gherardo.chirici@unimol.it; elapoint@fs.fed.us RI Chirici, Gherardo/E-6364-2010 OI Chirici, Gherardo/0000-0002-0669-5726 NR 110 TC 14 Z9 17 U1 9 U2 63 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD JUN PY 2012 VL 58 IS 3 SI SI BP 294 EP 309 DI 10.5849/forsci.10-075 PG 16 WC Forestry SC Forestry GA 959KE UT WOS:000305310500011 ER PT J AU Diez, JM D'Antonio, CM Dukes, JS Grosholz, ED Olden, JD Sorte, CJB Blumenthal, DM Bradley, BA Early, R Ibanez, I Jones, SJ Lawler, JJ Miller, LP AF Diez, Jeffrey M. D'Antonio, Carla M. Dukes, Jeffrey S. Grosholz, Edwin D. Olden, Julian D. Sorte, Cascade J. B. Blumenthal, Dana M. Bradley, Bethany A. Early, Regan Ibanez, Ines Jones, Sierra J. Lawler, Joshua J. Miller, Luke P. TI Will extreme climatic events facilitate biological invasions? SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Review ID DROUGHT; DISTURBANCE; IMPACTS; PLANTS; TOLERANCE; RIVER; CONSEQUENCES; TEMPERATURE; POPULATIONS; COEXISTENCE AB Extreme climatic events (ECEs) - such as unusual heat waves, hurricanes, floods, and droughts - can dramatically affect ecological and evolutionary processes, and these events are projected to become more frequent and more intense with ongoing climate change. However, the implications of ECEs for biological invasions remain poorly understood. Using concepts and empirical evidence from invasion ecology, we identify mechanisms by which ECEs may influence the invasion process, from initial introduction through establishment and spread. We summarize how ECEs can enhance invasions by promoting the transport of propagules into new regions, by decreasing the resistance of native communities to establishment, and also sometimes by putting existing non-native species at a competitive disadvantage. Finally, we outline priority research areas and management approaches for anticipating future risks of unwanted invasions following ECEs. Given predicted increases in both ECE occurrence and rates of species introductions around the globe during the coming decades, there is an urgent need to understand how these two processes interact to affect ecosystem composition and functioning. C1 [Diez, Jeffrey M.] ETH, Inst Integrat Biol, Zurich, Switzerland. [D'Antonio, Carla M.] Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Dukes, Jeffrey S.] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Grosholz, Edwin D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Olden, Julian D.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Sorte, Cascade J. B.] Univ Massachusetts, Dept Environm Earth & Ocean Sci, Boston, MA 02125 USA. [Blumenthal, Dana M.] ARS, Rangeland Resources Res Unit, USDA, Ft Collins, CO USA. [Bradley, Bethany A.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Early, Regan] Univ Evora, Catedra Rui Nabeiro, Evora, Portugal. [Ibanez, Ines] Univ Michigan, Sch Nat Resources, Ann Arbor, MI 48109 USA. [Jones, Sierra J.] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA. [Lawler, Joshua J.] Univ Washington, Sch Forest Resources, Seattle, WA 98195 USA. [Miller, Luke P.] Northeastern Univ, Ctr Marine Sci, Nahant, MA 01908 USA. RP Diez, JM (reprint author), ETH, Inst Integrat Biol, Zurich, Switzerland. EM jeffrey.diez@env.ethz.ch RI Dukes, Jeffrey/C-9765-2009; Bradley, Bethany/B-1964-2008; Blumenthal, Dana/J-3106-2012; Miller, Luke/B-8992-2009; OI Dukes, Jeffrey/0000-0001-9482-7743; Bradley, Bethany/0000-0003-4912-4971; Miller, Luke/0000-0002-2009-6981; Olden, Julian/0000-0003-2143-1187; Early, Regan/0000-0003-4108-5904 FU National Center for Ecological Analysis and Synthesis; National Science Foundation [EF-0553768]; University of California; Santa Barbara; State of California FX This review was conducted as part of the Climate Change & Invasive Species Working Group supported by the National Center for Ecological Analysis and Synthesis, a Center funded by the National Science Foundation (grant #EF-0553768), the University of California, Santa Barbara, and the State of California. NR 56 TC 89 Z9 92 U1 16 U2 243 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD JUN PY 2012 VL 10 IS 5 BP 249 EP 257 DI 10.1890/110137 PG 9 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 957NV UT WOS:000305170600016 ER PT J AU Chacko, N Gold, S AF Chacko, Nadia Gold, Scott TI Deletion of the Ustilago maydis ortholog of the Aspergillus sporulation regulator medA affects mating and virulence through pheromone response SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Ustilago maydis; medA; Pheromone response; Virulence; DelsGate; Sporulation ID LOOP-HELIX PROTEIN; CORN SMUT FUNGUS; TRANSCRIPTION FACTOR; PATHOGENIC DEVELOPMENT; TELIOSPORE FORMATION; FILAMENTOUS GROWTH; FUSARIUM-OXYSPORUM; SEXUAL DEVELOPMENT; GENE; NIDULANS AB Mating of compatible haploid cells of Ustilago maydis is essential for infection and disease development in the host. For mating and subsequent filamentous growth and pathogenicity, the transcription factor, prf1 is necessary. Prf1 is in turn regulated by the cAMP and MAPK pathways and other regulators like rop1 and hap1. Here we describe the identification of another putative Prf1 regulator, med1, the ortholog of the Aspergillus nidulans medusa (medA) transcription factor and show that it is required for mating and full virulence in U. maydis. med1 deletion mutants show both pre- and post-mating defects and are unresponsive to external pheromone. The expression of prf1 is down-regulated in Delta med1 compared to the wild type, suggesting that med1 is upstream of prf1. Additionally, indicative of a role in secondary metabolism regulation, deletion of the med1 gene de-represses the production of glycolipids in U. maydis. Published by Elsevier Inc. C1 [Gold, Scott] ARS, USDA, Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. [Chacko, Nadia; Gold, Scott] Univ Georgia, Dept Plant Pathol, Athens, GA 30605 USA. RP Gold, S (reprint author), ARS, USDA, Russell Res Ctr, Toxicol & Mycotoxin Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM sgold@uga.edu RI Chacko, Nadia/O-7834-2015 OI Chacko, Nadia/0000-0003-1817-2671 FU United States Department of Agriculture National Research Initiative [20033531913361] FX We thank Dr. David Andrews for technical assistance with complementation experiments. This work was supported by the United States Department of Agriculture National Research Initiative Competitive Grant No. 20033531913361 to S.E.G. NR 38 TC 4 Z9 5 U1 1 U2 21 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUN PY 2012 VL 49 IS 6 BP 426 EP 432 DI 10.1016/j.fgb.2012.04.002 PG 7 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 957MR UT WOS:000305167600002 PM 22537792 ER PT J AU Du, XH Zhao, Q O'Donnell, K Rooney, AP Yang, ZL AF Du, Xi-Hui Zhao, Qi O'Donnell, Kerry Rooney, Alejandro P. Yang, Zhu L. TI Multigene molecular phylogenetics reveals true morels (Morchella) are especially species-rich in China SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Ascomycota; Biodiversity; Conservation biology; DNA sequence; Fungi; Range evolution ID LONG-DISTANCE DISPERSAL; HISTORICAL BIOGEOGRAPHY; EVOLUTIONARY HISTORY; NORTHERN-HEMISPHERE; TEMPERATE FORESTS; DNA; BASIDIOMYCOTA; PATTERNS; FUNGI; DIVERSIFICATION AB The phylogenetic diversity of true morels (Morchella) in China was estimated by initially analyzing nuclear ribosomal internal transcribed spacer (ITS) rDNA sequences from 361 specimens collected in 21 provinces during the 2003-2011 growing seasons, together with six collections obtained on loan from three Chinese herbaria. Based on the results of this preliminary screen, 40 Esculenta Clade (yellow morels) and 30 Elata Clade (black morels) were chosen to represent the full range of phylogenetic diversity sampled. To investigate their species limits, we generated DNA sequences from portions of three protein-coding genes (RPB1, RPB2 and EF-1 alpha) and domains D1 and D2 of the nuclear large subunit (LSU) rDNA for all 70 collections. To fully assess evolutionary relationships, previously published multilocus DNA sequence data representing all known Morchella species was included in this study. Phylogenetic analyses employing maximum parsimony and maximum likelihood frameworks resolved 30 species in China compared with 22 in Europe and 19 within North America. Eleven novel phylogenetically distinct species were discovered in China, including two species within the Elata Clade and nine within the Esculenta Clade. Of the 30 species in China, 20 appear to be endemic, nine were also represented in Europe, and four putatively fire-adapted species have disjunct distributions in China, Europe and western North America. Although the diversification time estimates place the Esculenta Clade in China as early as the late Cretaceous and the Elata Clade by the early Oligocene, 27 of the 30 species evolved between the middle Miocene 12 Mya and present. (C) 2012 Elsevier Inc. All rights reserved. C1 [Du, Xi-Hui; Zhao, Qi; Yang, Zhu L.] Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650201, Yunnan Province, Peoples R China. [O'Donnell, Kerry; Rooney, Alejandro P.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Du, Xi-Hui] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. RP Yang, ZL (reprint author), Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Lanhei Rd 132, Kunming 650201, Yunnan Province, Peoples R China. EM fungiamanita@gmail.com FU National Natural Science Foundation of China [U0836604]; Yunnan Provincial Government [U0836604]; National Basic Research Program of China [2009CB522300]; Chinese Academy of Sciences; Key Laboratory of Biodiversity and Biogeography (KIB) [KBB-200805] FX We are very grateful to Prof. Y.J. Yao and Dr. T. Z. Wei, Institute of Microbiology of the Chinese Academy of Sciences (HMAS, Beijing), Prof. B. Tolgor, Mycological Institute of Jilin Agricultural University (HMJAU), Prof. G. Kost and Dr. K.-H. Rexer, University of Marburg, Germany (MB), and Dr. G.S. Barseghyan, University of Haifa, Israel (HAI) for sending us specimens on loan. Thanks are also due Drs. B. Feng, Y.C. Li and Z.W. Ge, Kunming Institute of Botany (KIB) for kindly providing specimens and photos. We also thank Stacy Sink, NCAUR-ARS-USDA, for excellent technical assistance and John Plischke III for the photo of Morchella rufobrunnea used in Fig. 5. This study was supported by the Joint Funds from the National Natural Science Foundation of China and Yunnan Provincial Government (No. U0836604), the National Basic Research Program of China (No. 2009CB522300), the Hundred Talents Program of the Chinese Academy of Sciences, and the Key Laboratory of Biodiversity and Biogeography (KIB, No. KBB-200805). The mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 65 TC 23 Z9 32 U1 10 U2 26 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUN PY 2012 VL 49 IS 6 BP 455 EP 469 DI 10.1016/j.fgb.2012.03.006 PG 15 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 957MR UT WOS:000305167600005 PM 22503770 ER PT J AU Pendinen, G Spooner, DM Jiang, JM Gavrilenko, T AF Pendinen, Galina Spooner, David M. Jiang, Jiming Gavrilenko, Tatjana TI Genomic in situ hybridization reveals both auto- and allopolyploid origins of different North and Central American hexaploid potato (Solanum sect. Petota) species SO GENOME LA English DT Article DE GISH; polyploids; potato; Solanum section Petota ID CULTIVATED POTATO; PLANT POLYPLOIDS; WILD POTATOES; RESISTANCE; PHYLOGENY; DNA; HJERTINGII; EVOLUTION; HOUGASII; RFLPS AB Wild potato (Solanum L. sect. Petota Dumort.) species contain diploids (2n = 2x = 24) to hexaploids (2n = 6x = 72). J.G. Hawkes classified all hexaploid Mexican species in series Demissa Bukasov and, according to a classic five-genome hypothesis of M. Matsubayashi in 1991, all members of series Demissa are allopolyploids. We investigated the genome composition of members of Hawkes's series Demissa with genomic in situ hybridization (GISH), using labeled DNA of their putative progenitors having diploid AA, BB, or PP genome species or with DNA of tetraploid species having AABB or AAA(a)A(a) genomes. GISH analyses support S. hougasii Correll as an allopolyploid with one AA component genome and another BB component genome. Our results also indicate that the third genome of S. hougasii is more closely related to P or a P genome-related species. Solanum demissum Lindl., in contrast, has all three chromosome sets related to the basic A genome, similar to the GISH results of polyploid species of series Acaulia Juz. Our results support a more recent taxonomic division of the Mexican hexaploid species into two groups: the allopolyploid Iopetala group containing S. hougasii, and an autopolyploid Acaulia group containing S. demissum with South American species S. acaule Bitter and S. albicans (Ochoa) Ochoa. C1 [Pendinen, Galina; Gavrilenko, Tatjana] NI Vavilov Inst Plant Ind, Dept Biotechnol, St Petersburg 190000, Russia. [Spooner, David M.] Univ Wisconsin, USDA, ARS, Dept Hort, Madison, WI 53706 USA. [Jiang, Jiming] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Gavrilenko, Tatjana] St Petersburg State Univ, St Petersburg, Russia. RP Gavrilenko, T (reprint author), NI Vavilov Inst Plant Ind, Dept Biotechnol, Bolshaya Morskaya St 42-44, St Petersburg 190000, Russia. EM tatjana9972@yandex.ru FU International Science and Technology Center [3329]; NSF DEB [0316614]; USDA NRI [2008-35300-18669] FX This research was supported by International Science and Technology Center Grant 3329 to T.G., G.P., and D.M.S. and by NSF DEB 0316614 and USDA NRI 2008-35300-18669 to D.M.S. The authors thank I. Golubovskaya for helpful suggestions to the GISH data analysis and for reading the manuscript, and H. Ruess and V. Chernov for laboratory assistance. NR 38 TC 7 Z9 7 U1 0 U2 20 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0831-2796 J9 GENOME JI Genome PD JUN PY 2012 VL 55 IS 6 BP 407 EP 415 DI 10.1139/G2012-027 PG 9 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 959EF UT WOS:000305294800001 PM 22594521 ER PT J AU Kumar, A Lee, WS Ehsani, RJ Albrigo, LG Yang, CH Mangan, RL AF Kumar, Arun Lee, Won Suk Ehsani, Reza J. Albrigo, L. Gene Yang, Chenghai Mangan, Robert L. TI Citrus greening disease detection using aerial hyperspectral and multispectral imaging techniques SO JOURNAL OF APPLIED REMOTE SENSING LA English DT Article DE Huanglongbing; linear spectral unmixing; mixture tuned matched filtering; spectral angle mapping ID REFLECTANCE AB Airborne multispectral and hyperspectral imaging can be used to detect potentially diseased trees rapidly over a large area using unique spectral signatures. Ground inspection and management can be focused on these detected zones, rather than an entire grove, making it less labor-intensive and time-consuming. We propose a method to detect the areas of citrus groves infected with citrus greening disease [Huanglongbing (HLB)] using airborne hyperspectral and multispectral imaging. This would prevent further spread of infection with efficient management plans of infected areas. Two sets of hyperspectral images were acquired in 2007 and 2009, from different citrus groves in Florida. Multispectral images were acquired only in 2009. A comprehensive ground truthing based on ground measurements and visual check of the citrus trees was used for validating the results using 2007 images. In 2009, a more accurate polymerase chain reaction test for selected trees from ground truthing was carried out. With a handheld spectrometer, ground spectral measurements were obtained along with their degrees of infection. A hyperspectral imaging software (ENVI, ITT VIS) was used for the analysis. HLB infected areas were identified using image-derived spectral library, mixture tuned matched filtering (MTMF), spectral angle mapping (SAM), and linear spectral unmixing. The accuracy of the MTMF method was greater than the other methods. The accuracy of SAM using multispectral images (87%) was comparable to the results of the MTMF and also yielded higher accuracy when compared to SAM analysis on hyperspectral images. A possible inaccurate ground truthing for the grove in 2007 generated more false positives. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JRS.6.063542] C1 [Kumar, Arun; Lee, Won Suk] Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. [Ehsani, Reza J.] Univ Florida, Citrus Res & Educ Ctr, IFAS, Lake Alfred, FL 33850 USA. [Yang, Chenghai; Mangan, Robert L.] ARS, USDA, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Albrigo, L. Gene] Univ Florida, Citrus Res & Educ Ctr, Dept Hort, Lake Alfred, FL 33850 USA. [Yang, Chenghai] ARS, USDA, Kika de la Garza Agr Res Ctr, Weslaco, TX USA. RP Kumar, A (reprint author), Univ Florida, Agr & Biol Engn Dept, Gainesville, FL 32611 USA. EM wslee@ufl.edu FU Citrus Research and Development Foundation, Inc. FX The authors would like to thank Mr. Ashish Mishra, Ms. Sherrie Buchanon, Ms. Ujwala Jadhav, and Mr. Eduardo Chica at the CREC in the University of Florida, Mr. Anurag Katti in the Electrical and Computer Engineering in the University of Florida, Dr. Eran Raveh at the Agricultural Research Organization, Israel, and Mr. James Colee, UF/IFAS statistical consultant, for their assistance. We also thank Mr. Michael Ohlinger of Sterling Air Service at Weslaco, TX, and Mr. Fred Gomez of USDA-ARS at Weslaco, TX, for taking the multispectral and hyperspectral imagery in 2009 and Mr. Jim Forward of USDA-ARS at Weslaco, TX, for preprocessing the imagery. This project was funded by the Citrus Research and Development Foundation, Inc. NR 24 TC 14 Z9 14 U1 1 U2 27 PU SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS PI BELLINGHAM PA 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98225 USA SN 1931-3195 J9 J APPL REMOTE SENS JI J. Appl. Remote Sens. PD JUN 1 PY 2012 VL 6 AR 063542 DI 10.1117/1.JRS.6.063542 PG 22 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 960PN UT WOS:000305400500001 ER PT J AU Pomares, C Santin, M Miegeville, M Espern, A Albano, L Marty, P Morio, F AF Pomares, Christelle Santin, Monica Miegeville, Michel Espern, Anne Albano, Laetitia Marty, Pierre Morio, Florent TI A New and Highly Divergent Enterocytozoon bieneusi Genotype Isolated from a Renal Transplant Recipient SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID MICROSPORIDIOSIS; SEQUENCE; AIDS AB A 49-year-old renal transplant recipient was admitted to our hospital due to abundant liquid diarrhea and dehydration. Parasitological investigations, including genotyping, led to the diagnosis of intestinal microsporidiosis due to a new and highly divergent internal transcribed spacer (ITS) genotype of Enterocytozoon bieneusi. The potential route of transmission through horse stools is discussed. C1 [Pomares, Christelle; Marty, Pierre] Ctr Mediterraneen Med Mol, C3M, INSERM, U895, Nice, France. [Pomares, Christelle; Marty, Pierre] Ctr Hosp Univ Nice, Serv Parasitol Mycol, Nice, France. [Santin, Monica] ARS, Environm Microbial & Food Safety Lab, Anim & Nat Resources Inst, USDA, Beltsville, MD USA. [Miegeville, Michel; Morio, Florent] Nantes Atlantique Univ, Univ Nantes, Dept Parasitol & Mycol Med, Fac Pharm,IICIMed EA 1155, Nantes, France. [Miegeville, Michel; Espern, Anne; Morio, Florent] Ctr Hosp Univ Nantes, Lab Parasitol Mycol, Nantes, France. [Albano, Laetitia] Nephrol Unite Med Chirurg Transplantat Renale CHU, Nice, France. RP Pomares, C (reprint author), Ctr Mediterraneen Med Mol, C3M, INSERM, U895, Nice, France. EM pomares.c@chu-nice.fr NR 15 TC 6 Z9 7 U1 0 U2 2 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUN PY 2012 VL 50 IS 6 BP 2176 EP 2178 DI 10.1128/JCM.06791-11 PG 3 WC Microbiology SC Microbiology GA 956VZ UT WOS:000305119000073 PM 22442321 ER PT J AU Martinson, K Jung, H Hathaway, M Sheaffer, C AF Martinson, Krishona Jung, Hans Hathaway, Marcia Sheaffer, Craig TI The Effect of Soaking on Carbohydrate Removal and Dry Matter Loss in Orchardgrass and Alfalfa Hays SO JOURNAL OF EQUINE VETERINARY SCIENCE LA English DT Article DE Alfalfa; Dry Matter Loss; Hay Soaking; Nonstructural Carbohydrate Fractions; Orchardgrass ID EQUINE LAMINITIS; NUTRITIVE-VALUE; QUALITY; YIELD; LEGUME; OLIGOFRUCTOSE; RAINFALL; GLUCOSE; FORAGES; HORSES AB The objective of this research was to determine the impact of water temperature and soaking length on removal of nonstructural carbohydrate (NSC) fractions and dry matter (DM) from legume and cool-season grass hays. The experimental design was a randomized complete block with six replications. Hay types included bud and flowering alfalfa (Medicago sativa L) and vegetative and flowering orchardgrass (Dactylis glomerata L). Flakes were submerged for 15, 30, and 60 minutes in 25 L of cold (22 degrees C) water, warm (39 degrees C) water, and 12 hours in cold water, respectively. Water temperature at each soaking duration did not affect residual NSC fractions in alfalfa hays. Flowering and vegetative orchardgrass hays soaked for 30 and 60 minutes, respectively, in warm water had greater (P < .001) amounts of NSC fraction removal compared with soaking in cold water. Soaking bud and flowering alfalfa and vegetative orchardgrass hays for >= 15 minutes removed more NSC fractions compared with the control (P <= .005); however, soaking for 15-60 minutes resulted in similar NSC fraction removal. Conversely, in flowering orchardgrass hay, longer soaking times resulted in greater (P < .001) NSC fraction removal. Fructan removal in orchardgrass hay was greatest (P < .001) after soaking for 12 hours. DM losses were similar among hays after soaking for 15-60 minutes, but were greatest after soaking for 12 hours (P < .001). Soaking grass hay for 15-30 minutes is recommended to remove sufficient NSC fractions while minimizing DM losses. (C) 2012 Elsevier Inc. All rights reserved. C1 [Martinson, Krishona; Jung, Hans; Hathaway, Marcia] Univ Minnesota, Dept Anim Sci, St Paul, MN 55108 USA. [Jung, Hans] USDA ARS, St Paul, MN USA. [Jung, Hans; Sheaffer, Craig] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA. RP Martinson, K (reprint author), Univ Minnesota, Dept Anim Sci, 1364 Eckles Ave, St Paul, MN 55108 USA. EM krishona@umn.edu NR 37 TC 6 Z9 6 U1 2 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0737-0806 J9 J EQUINE VET SCI JI J. Equine Vet. Sci. PD JUN PY 2012 VL 32 IS 6 BP 332 EP 338 DI 10.1016/j.jevs.2011.11.009 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA 959LC UT WOS:000305312900005 ER PT J AU Bowker, BC Fahrenholz, TM Sarnoski, PJ Solomon, MB AF Bowker, Brian C. Fahrenholz, Timothy M. Sarnoski, Paul J. Solomon, Morse B. TI Alterations in the Sarcoplasmic Protein Fraction of Beef Muscle with Postmortem Aging and Hydrodynamic Pressure Processing SO JOURNAL OF FOOD SCIENCE LA English DT Article DE aging; beef; hydrodynamic pressure processing; sarcoplasmic proteins; tenderness ID STRIP LOINS; GEL-ELECTROPHORESIS; PIG MEAT; TENDERNESS; IDENTIFICATION; DEGRADATION; PORK; MYOFIBRILLAR; PROTEOLYSIS; PROTEASES AB Capillary electrophoresis (CE) and reversed-phase high performance liquid chromatography (RP-HPLC) analyses were utilized to detect differences in the sarcoplasmic protein fractions of beef strip loins subjected to aging and hydrodynamic pressure processing (HDP) treatments. At 48 h postmortem, strip loins (n = 12) were halved and subjected to control or HDP treatments. Following treatment, each half was divided into 3 portions which were aged for 0, 5, and 8 d. After each aging period, steaks were removed for Warner-Bratzler shear force (WBSF) analysis and for the extraction of sarcoplasmic proteins which were analyzed by CE and RP-HPLC. Aging by HDP interactions were not detected using either separation technique. With CE analysis, no HDP effects were observed; however, the relative peak area of 8 protein peaks ranging in size from 17 to > 200 kDa were influenced by postmortem aging. Separation of proteins by RP-HPLC demonstrated that HDP influenced the relative size of 2 protein peaks while postmortem aging effects were observed in 6 peaks. Alterations in the sarcoplasmic protein fractions detected by both CE and RP-HPLC were correlated to WBSF measurements. Overall, data demonstrate that HDP has minimal effects on sarcoplasmic proteins and that aging related changes in the water soluble protein fractions of muscle may be useful as indirect indicators of beef tenderness. C1 [Bowker, Brian C.] ARS, USDA, Athens, GA 30605 USA. [Fahrenholz, Timothy M.; Sarnoski, Paul J.; Solomon, Morse B.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Bowker, BC (reprint author), ARS, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM brian.bowker@ars.usda.gov NR 29 TC 3 Z9 4 U1 2 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD JUN PY 2012 VL 77 IS 6 BP C594 EP C602 DI 10.1111/j.1750-3841.2012.02735.x PG 9 WC Food Science & Technology SC Food Science & Technology GA 952SQ UT WOS:000304813100003 PM 22671519 ER PT J AU Smith, BM Bean, SR Herald, TJ Aramouni, FM AF Smith, B. M. Bean, S. R. Herald, T. J. Aramouni, F. M. TI Effect of HPMC on the Quality of Wheat-Free Bread Made from Carob Germ Flour-Starch Mixtures SO JOURNAL OF FOOD SCIENCE LA English DT Article DE carob; celiac; dough; gluten-free; wheat-free ID GLUTEN-FREE BREAD; ZEIN; FUNCTIONALITY; RHEOLOGY; PROTEINS; KAFIRIN; SORGHUM; DOUGH AB Carob germ proteins have been shown to have functional properties similar to wheat gluten enabling formulation and production of yeast leavened gluten-free baked goods from a true dough rather than a stiff batter. The purpose of this research was to optimize the production of wheat-free bread containing carob germ flour, corn starch, NaCl, sucrose, hydroxypropyl methylcellulose (HPMC), and H2O. A key criterion was to formulate viscoelastic dough similar to wheat dough. To that end, response surface methodology (RSM) was used to determine optimal levels of carob germ flour, H2O, and HPMC. Components varied as follows: 4.94%-15.05% for carob germ flour, 0.05%-3.75% HPMC, and 65.25%-83.75% H2O (percents are on a flour basis, where carob germ flour in combination with maize starch equals 100%). Sucrose, NaCl, and yeast were held constant at 2%. Bread parameters evaluated were specific volume and crumb hardness, where the largest specific volume and the lowest value for crumb hardness were considered most desirable. The optimum formula as determined by RSM consisted of 7% carob germ flour, 93% maize starch, 2% HPMC, and 80% H2O with predicted crumb hardness of similar to 200 g of force and a specific volume of similar to 3.5 cm(3)/g. When proof time was optimized, a specific volume of similar to 5.6 ml/g and crumb hardness value of similar to 156 g of force was observed. Carob germ flour may be used as an alternative to wheat flour in formulating viscoelastic dough and high quality gluten-free bread. C1 [Smith, B. M.; Bean, S. R.; Herald, T. J.] ARS, USDA, CGAHR, Manhattan, KS 66502 USA. [Aramouni, F. M.] Kansas State Univ, Inst Food Sci, Ksu Manhattan, KS 66506 USA. RP Bean, SR (reprint author), ARS, USDA, CGAHR, 1515 Coll Ave, Manhattan, KS 66502 USA. EM scott.bean@ars.usda.gov OI Bean, Scott/0000-0001-8678-8094 NR 17 TC 9 Z9 9 U1 1 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD JUN PY 2012 VL 77 IS 6 BP C684 EP C689 DI 10.1111/j.1750-3841.2012.02739.x PG 6 WC Food Science & Technology SC Food Science & Technology GA 952SQ UT WOS:000304813100017 PM 22671523 ER PT J AU Mason, L White, G Morishima, G Alvarado, E Andrew, L Clark, F Durglo, M Durglo, J Eneas, J Erickson, J Friedlander, M Hamel, K Hardy, C Harwood, T Haven, F Isaac, E James, L Kenning, R Leighton, A Pierre, P Raish, C Shaw, B Smallsalmon, S Stearns, V Teasley, H Weingart, M Wilder, S AF Mason, Larry White, Germaine Morishima, Gary Alvarado, Ernesto Andrew, Louise Clark, Fred Durglo, Mike, Sr. Durglo, Jim Eneas, John Erickson, Jim Friedlander, Margaret Hamel, Kathy Hardy, Colin Harwood, Tony Haven, Faline Isaac, Everett James, Laurel Kenning, Robert Leighton, Adrian Pierre, Pat Raish, Carol Shaw, Bodie Smallsalmon, Steven Stearns, Vernon Teasley, Howard Weingart, Matt Wilder, Spus TI Listening and Learning from Traditional Knowledge and Western Science: A Dialogue on Contemporary Challenges of Forest Health and Wildfire SO JOURNAL OF FORESTRY LA English DT Article DE fire history; traditional knowledge; indigenous knowledge; Native American forestry; forest fuels ID ECOLOGICAL KNOWLEDGE; INDIGENOUS KNOWLEDGE; MANAGEMENT AB Native Americans relied on fire to maintain a cultural landscape that sustained their lifeways for thousands of years. Within the past 100 years, however, policies of fire exclusion have disrupted ecological processes, elevating risk of wildfire, insects, and disease, affecting the health and availability of resources on which the tribes depend. On Indian Reservations, tribal forest plans include prescribed fire to restore and maintain the lands. Public land managers are now considering ways to restore the fire-based ecosystem, but tribal knowledge about the use and effects of fire has largely been left out of the discussion. For 2 days in June 2010, 7 tribal elders joined with 20 native and nonnative scientists, resource managers, and academics to explore ways to integrate Native American stewardship practices, traditional knowledge, and philosophies with western science to address contemporary forest health and wildfire challenges. The workshop, convened on the Flathead Indian Reservation of the Confederated Salish Kootenai Tribes located in western Montana, provided a forum for candid dialogue and knowledge sharing. This article, coauthored by all 27 participants, offers a summary background followed by candid highlights of dialogue along with recommendations for progress based on lessons learned. The central conclusion is that integration and application of traditional knowledge with western science for improved stewardship of natural resources will require enduring commitments to knowledge sharing that extend beyond the usual boundaries of professional training and cultural orientation such that learning can proceed, legacy myths might be corrected, and the forests and the people will benefit. C1 [Mason, Larry; Alvarado, Ernesto; Wilder, Spus] Univ Washington, Sch Forest Resources, Seattle, WA 98195 USA. [Clark, Fred; Haven, Faline] USDA Forest Serv, Off Tribal Affairs, Washington, DC 20024 USA. [White, Germaine; Durglo, Jim; Harwood, Tony] Kootenai Tribes Forestry Dept, Ronan, MT 59864 USA. [Hardy, Colin] USDA Forest Serv, Rocky Mt Res Stn, Fire Fuels & Smoke Program, Missoula Fire Lab, Missoula, MT 59808 USA. [Isaac, Everett; James, Laurel] Univ Washington, Sch Forest Resources, Seattle, WA 98125 USA. [Kenning, Robert; Leighton, Adrian; Weingart, Matt] Salish Kootenai Coll, Pablo, MT 59855 USA. [Raish, Carol] USDA Forest Serv Rocky Mt Res Stn, Albuquerque, NM 87102 USA. [Shaw, Bodie] Natl Interagency Fire Ctr, Boise, ID 83705 USA. [Shaw, Bodie] USDI Bur Indian Affairs, Boise, ID 83705 USA. [Stearns, Vernon] Spokane Tribal Fire Management, Wellpinit, WA 99040 USA. [Teasley, Howard] Nez Perce Forest Tribal Forestry, Lapwai, ID 83540 USA. RP Mason, L (reprint author), Univ Washington, Sch Forest Resources, Box 352100, Seattle, WA 98195 USA. EM larrym@uw.edu; germainew@cskt.org; MORIKOG@aol.com; alvarado@uw.edu; fclark@fs.fed.us; jimd@cskt.org; jim.erickson@couleedam.net; chardy01@fs.fed.us; tonyh@cskt.org; fhaven@fs.fed.us; eisaac@uw.edu; laurelj@uw.edu; robert_kenning@skc.edu; adrian_leighton@skc.edu; craish@fs.fed.us; bodie_shaw@nifc.gov; VearnS@Spokanetribe.com; howardt@nezperce.org; mathewweingart@student.skc.edu; spuswilder@u.washington.edu FU Joint Fire Science Program; University of Washington; Rocky Mountain Research Station FX Larry Mason (larrym@uw.edu) is resource scientist and outreach coordinator at the University of Washington, School of Forest Resources, Box 352100, Seattle, WA 98195. Germaine White (germainew@cskt.org) is Confederated Salish Kootenai Tribes and Information and education specialist for the Confederated Salish and Kootenai Tribes Natural Resources Department, 104 Main Street, Ronan, MT 59864. Gary Morishima (MORIKOG@aol.com) is a founding member of the Intertribal Timber Council and the Natural Resource Technical Specialist for the Quinault Indian Nation, 3010 77th Avenue SE Suite 104, Mercer Island, WA 98040. Ernesto Alvarado (alvarado@uw.edu) associate professor of Fire Ecology at the University of Washington, School of Forest Resources, Box 352100, Seattle, WA 98125. Louise Andrew is a Kootenai Tribal Elder and member of the Kootenai Culture Committee, Confederated Salish and Kootenai Tribes, PO Box 278, Pablo, MT 59855. Fred Clark (filark@fifed.us) is Citizen Potawatomi Nation and director of the Office of Tribal Affairs, USDA Forest Service, 1400 Independence Avenue SW Washington DC 20024. Mike Durglo Sr. is a Salish Pend d'Oreille Tribal Elder and member of the Salish-Pend d'Oreille Culture Committee, Confederated Salish and Kootenai Tribes, PO Box 550, St. Ignatius, MT 59865. Jim Durglo (jimd@cskt.org) is Confederated Salish and Kootenai Tribes and Forest Manager, Confederated Salish and Kootenai Tribes Forestry Department, 104 Main Street, Ronan, MT 59864. John Eneas is a Kootenai Tribal Elder and member of the Kootenai Culture Committee, Confederated Salish and Kootenai Tribes, PO Box 278, Pablo, MT 59855. Jim Erickson (jim.erickson@couleedam.net) is the fire specialist for the Intertribal Timber Council, 707 Spruce Street, Coulee Dam, WA 99116 Margaret Friedlander and Kathy Hamel are Kootenai Tribal Elders and members of the Kootenai Culture Committee, Confederated Salish and Kootenai Tribes, PO Box 278, Pablo, MT 59855. Colin Hardy (chardy01@fs.fed.us) is program manager fir the Fire, Fuels, and Smoke Program at the Missoula Fire Laboratory of the USDA Forest Service Rocky Mountain Research Station, 5775 US Highway 10 West, Missoula MT 59808. Tony Harwood (tonyh@cskt.org) is Confederated Salish and Kootenai Tribes and forestry technical specialist, Confederated Salish and Kootenai Tribes Forestry Department, 104 Main Street, Ronan, MT 59864. Faline Haven (fhaven@filed.us) is Metlakatla Indian Community and Management Analyst for the Office of Tribal Affairs, USDA Forest Service, 1400 Independence Avenue SW Washington, DC 20024. Everett Isaac (eisaac@uw.edu) and Laurel James (laurelj@uw.edu) are Yakima Indian Nation and graduate students at the University of Washington, School of Forest Resources, Box 352100, Seattle, WA 98125. Robert Kenning (robert_kenning@skc.edu) and Adrian Leighton (adrian_leighton@skc.edu) are forestry instructors at Salish Kootenai College, PO Box 70, Pablo, MT 59855. Pat Pierre is a Salish Pend d'Oreille Tribal Elder and member of the Salish-Pen d d'Oreille Culture Committee, Confederated Salish and Kootenai Tribes, PO Box 550, St. Ignatius, MT 59865. Carol Raish (craish@fs.fed.us) is research social scientist with the USDA Forest Service Rocky Mountain Research Station, 333 Broadway SE, Suite 115, Albuquerque, NM 87102. Bodie Shaw (bodie_shaw@nifi.gov) is Confederated Tribes of Warm Springs and is deputy director of the USDI Bureau of Indian Affairs and the National Interagency Fire Center, 3833 South Development Avenue, Boise, ID 83705.; Steven Smallsalmon is a Salish Pend d'Oreille Tribal Elder and member of the Salish-Pend d'Oreille Culture Committee, Confederated Salish and Kootenai Tribes, PO Box 550, St. Ignatius, MT 59865. Vernon Stearns Jr. (VearnS@Spokanetribe.com) is Spokane Tribe of Indians and fuels manager, Spokane Tribal Fire Management, PO Box 389, Wellpinit, WA 99040. Howard Teasley (howardt@nezperce.olg) is Nez Perce Tribe and allotment forester, Nez Perce Forest Tribal Forestry, PO Box 365, Lapwai, ID 83540. Matt Weingart (matheroweingart@student.skc.edu) is Confederated Salish and Kootenai Tribes and an undergraduate student at Salish Kootenai College, PO Box 70, Pablo, MT 59855. Spits Wilder (spuswilder@u.washington.edu) is Colville Confederated Tribes and is a graduate student at the University ofWashington, School of Forest Resources, Box 352100, Seattle, WA 98125. The authors are gratefiil for the hospitality and generosity of the people of the Confederated Salish Kootenai Tribes. Inspiration fir this workshop evolved from the Native People and Fire in the Northern Rockies Project (www.cskt.org/fire_histoty.suif), which received the 2005 National Fire Pfrm Award for Excellence in Community Assistance Funding support for this project was provided through the Joint Fire Science Program, the University of Washington, and the Rocky Mountain Research Station. NR 47 TC 16 Z9 16 U1 4 U2 61 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2012 VL 110 IS 4 BP 187 EP 193 DI 10.5849/jof.11-006 PG 7 WC Forestry SC Forestry GA 957LK UT WOS:000305164300002 ER PT J AU Kleinman, SJ DeGomez, TE Snider, GB Williams, KE AF Kleinman, Sabrina J. DeGomez, Thomas E. Snider, Gary B. Williams, Kelly E. TI Large-Scale Pinyon Ips (Ips confusus) Outbreak in Southwestern United States Tied with Elevation and Land Cover SO JOURNAL OF FORESTRY LA English DT Article DE ips confusus; pinyon-juniper woodlands; drought; forest entomology; forest resources; forest management; Southwest ID TREE MORTALITY; SEVERE DROUGHT AB During a severe drought, a large-scale outbreak of pinyon ips between 2002 and 2004 in the southwestern United States, resulted in over 3 million a( of damage to pinyon pine forests in the southwestern United States. Previous studies suggest that damage was most severe in stands that encroached in lower elevation ecosystems. To gain a regional perspective on the outbreak, we did a geographical analysis of ips damage in association with land cover and elevation. Our analysis indicates that the overall distribution of the ips damage mirrors the distribution of pinyon-juniper woodlands in the region, with more intense damage occurring at higher elevations in Utah and Arizona, where pinyon pines are more common and have likely become denser with time. Our results suggest during droughts even historical stands of pinyon pine are at risk of pinyon ips damage, not just stands at the ecological extreme. C1 [Kleinman, Sabrina J.; DeGomez, Thomas E.; Williams, Kelly E.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Snider, Gary B.] USDA Forest Serv, Williams, AZ USA. RP Kleinman, SJ (reprint author), Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. EM sabklein@arizona.edu; degomez@ag.arizona.edu; gsnider02@fs.fed.us; kwilli@email.arizona.edu FU USDA Forest Service [07-CS-11031600-174] FX Sabrina J. Kleinman (sabklein@arizona.edu) is research specialist, and Thomas E. DeGomez (degomez@ag.arizona.edu) is extension forest health specialist, University of Arizona School of Natural Resources and the Environment, Biological Sciences East, Tucson, AZ 85721. Gary B. Snider (gsnider02@fs.fed.us) is natural resource planner, USDA Forest Service, the Kaibab National Forest, Williams, Arizona. Kelly E. Williams (kwilli@email.arizona.edu) is research specialist, University of Arizona School of Natural Resources and the Environment, Biological Sciences East, Tucson, AZ 85721. This project was funded through USDA Forest Service agreement No. 07-CS-11031600-174 as part of the Forest Health Monitoring Program of Forest Health Protection. The authors are grateful for the guidance and input of John Anhold and Joel McMillin and the staff from the USDA Forest Service Forest Health Program for the Southwest Region during this project. NR 21 TC 8 Z9 8 U1 2 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2012 VL 110 IS 4 BP 194 EP 200 DI 10.5849/jof.11-060 PG 7 WC Forestry SC Forestry GA 957LK UT WOS:000305164300003 ER PT J AU Van Fleet, TE Kittredge, DB Butler, BJ Catanzaro, PF AF Van Fleet, Tyler E. Kittredge, David B. Butler, Brett J. Catanzaro, Paul F. TI Reimagining Family Forest Conservation: Estimating Landowner Awareness and Their Preparedness to Act with the Conservation Awareness Index SO JOURNAL OF FORESTRY LA English DT Article DE family forest owner; forest conservation; awareness; assessment ID OWNERS; MANAGEMENT AB The Conservation Awareness Index (CAI) is a new, necessary survey instrument designed to assess family forest conservation progress. This article describes the rationale, development, and pilot testing of the CAI, which estimates family forest owner awareness (including familiarity, knowledge, and experience) of forest conservation options and sources of information. Administered to 500 randomly selected Massachusetts family forest owners and 64 benchmark landowners, results indicated instrument validity and revealed low conservation awareness among random respondents, especially regarding estate planning and conservation easements. Distance from land, education level, ownership size, and location were related to conservation awareness. Applications of the CAI include understanding family forest owner preparedness to make informed decisions about their land, improving outreach interventions, and measuring the spatial and social dynamics of conservation awareness over time. C1 [Van Fleet, Tyler E.] Univ Massachusetts, USDA Forest Serv, Amherst Family Forest Res Ctr, Amherst, MA 01003 USA. [Kittredge, David B.; Catanzaro, Paul F.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Butler, Brett J.] USDA Forest Serv, No Res Stn, Amherst, MA 01003 USA. RP Van Fleet, TE (reprint author), Univ Massachusetts, USDA Forest Serv, Amherst Family Forest Res Ctr, 160 Holdsworth Way, Amherst, MA 01003 USA. EM Tyler.Vanfleet@gmail.com; dbk@eco.umass.edu; bbutler01@fs.fed.us; cat@umext.umass.edu FU National Institute of Food and Agriculture, USDA; Massachusetts Agricultural Experiment Station; Department of Environmental Conservation [MAS0011] FX Tyler E Van Fleet (Tyler.Vanfleet@gmail.com) is research assistant, USDA Forest Service/University of Massachusetts Amherst Family Forest Research Center, 160 Holdsworth Way, Amherst, MA 01003, and David B. Kittredge (dbk@eco.umass.edu) is professor and extension forester, Department of Environmental Conservation, University of Massachusetts-Amherst, 160 Holdsworth Way, Amherst, MA 01003. Brett J. Butler (bbutler01@fs.fed.us) is research forester, USDA Forest Service, Northern Research Station, 160 Holdsworth Way, Amherst, MA 01003. Paul F. Catanzaro (cat@umext.umass.edu) is extension assistant professor, Department of Environmental Conservation, University of Massachusetts-Amherst, 160 Holdsworth Way, Amherst, MA 01003. This material is based on work supported by the National Institute of Food and Agriculture, USDA, the Massachusetts Agricultural Experiment Station, and the Department of Environmental Conservation under Project No. MAS0011. This project has greatly benefited from information and guidance from Charles M Schweik, Family Forest Research Center members, 2010 Keystone Project cooperators, and the many landowners and forest conservation experts who participated in the study. NR 33 TC 5 Z9 5 U1 1 U2 18 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUN PY 2012 VL 110 IS 4 BP 207 EP 215 DI 10.5849/jof.11-021 PG 9 WC Forestry SC Forestry GA 957LK UT WOS:000305164300005 ER PT J AU Welker, TL Lim, C Yildirim-Aksoy, M Klesius, PH AF Welker, Thomas L. Lim, Chhorn Yildirim-Aksoy, Mediha Klesius, Phillip H. TI Use of Diet Crossover to Determine the Effects of beta-glucan Supplementation on Immunity and Growth of Nile Tilapia, Oreochromis niloticus SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID MACROPHAGE RESPIRATORY BURST; CATFISH ICTALURUS-PUNCTATUS; BASS DICENTRARCHUS-LABRAX; TROUT ONCORHYNCHUS-MYKISS; CHANNEL CATFISH; EDWARDSIELLA-ICTALURI; DEFENSE-MECHANISMS; GILTHEAD SEABREAM; INNATE IMMUNITY; IN-VITRO AB Juvenile Nile tilapia were fed either a basal (control) diet (n = 6 aquaria) or a diet supplemented with 1 g/kg beta-glucan (n = 24 aquaria) for 4 wk. At the end of this period, fish receiving beta-glucan were continued on the same diet (n = 12 aquaria) or switched to the control diet (n = 12 aquaria) for 2 wk. After 6 wk, tilapia continuously fed the beta-glucan supplemented diets had improved weight gain and feed efficiency than those fed the control diet uninterrupted or switched from the beta-glucan diet to the control after 4 wk. Feeding tilapia beta-glucan for 4 wk and then switching to the basal diet for 2 wk caused a significant increase in the respiratory burst of polymorphonuclear lymphocytes (17.77 X 103 units/1000 white blood cells [WBC]) compared to catfish fed the control diet (13.50 X 103 units/1000 WBC) or the beta-glucan diet continuously (13.57 X 103 units/1000 WBC), but other immune parameters were unaffected. Tilapia were then challenged with Streptococcus iniae. The two groups were divided again (n = 6 aquaria) postchallenge and continued on the same diet or switched to the other diet (beta-glucan or control) for another 3 wk. No differences in survival to S. iniae infection occurred between dietary groups. C1 [Welker, Thomas L.] ARS, USDA, Hagerman Fish Culture Expt Stn, Hagerman, ID USA. [Lim, Chhorn; Yildirim-Aksoy, Mediha; Klesius, Phillip H.] ARS, USDA, Aquat Anim Hlth Res Lab, Auburn, AL USA. RP Welker, TL (reprint author), ARS, USDA, Hagerman Fish Culture Expt Stn, Hagerman, ID USA. NR 41 TC 3 Z9 3 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD JUN PY 2012 VL 43 IS 3 BP 335 EP 348 DI 10.1111/j.1749-7345.2012.00569.x PG 14 WC Fisheries SC Fisheries GA 956CO UT WOS:000305067600003 ER PT J AU Gladue, DP Holinka, LG Largo, E Sainz, IF Carrillo, C O'Donnell, V Baker-Branstetter, R Lu, ZG Ambroggio, X Risatti, GR Nieva, JL Borca, MV AF Gladue, Douglas P. Holinka, Lauren G. Largo, Eneko Sainz, Ignacio Fernandez Carrillo, Consuelo O'Donnell, Vivian Baker-Branstetter, Ryan Lu, Zhigiang Ambroggio, Xavier Risatti, Guillermo R. Nieva, Jose L. Borca, Manuel V. TI Classical Swine Fever Virus p7 Protein Is a Viroporin Involved in Virulence in Swine SO JOURNAL OF VIROLOGY LA English DT Article ID HEPATITIS-C VIRUS; HOG-CHOLERA VIRUS; INFLUENZA-A VIRUS; ION-CHANNEL; STRUCTURE PREDICTION; HIGHLY VIRULENT; STRAIN BRESCIA; GLYCOPROTEIN; PESTIVIRUS; DIFFERENTIATION AB The nonstructural protein p7 of classical swine fever virus (CSFV) is a small hydrophobic polypeptide with an apparent molecular mass of 6 to 7 kDa. The protein contains two hydrophobic stretches of amino acids interrupted by a short charged segment that are predicted to form transmembrane helices and a cytosolic loop, respectively. Using reverse genetics, partial in-frame deletions of p7 were deleterious for virus growth, demonstrating that CSFV p7 function is critical for virus production in cell cultures. A panel of recombinant mutant CSFVs was created using alanine scanning mutagenesis of the p7 gene harboring sequential three- to six-amino-acid residue substitutions spanning the entire protein. These recombinant viruses allowed the identification of the regions within p7 that are critical for virus production in vitro. In vivo, some of these viruses were partially or completely attenuated in swine relative to the highly virulent parental CSFV Brescia strain, indicating a significant role of p7 in CSFV virulence. Structure-function analyses in model membranes emulating the endoplasmic reticulum lipid composition confirmed that CSFV p7 is a pore-forming protein, and that pore-forming activity resides in the C-terminal transmembrane helix. Therefore, p7 is a viroporin which is clearly involved in the process of CSFV virulence in swine. C1 [Gladue, Douglas P.; Holinka, Lauren G.; Sainz, Ignacio Fernandez; O'Donnell, Vivian; Baker-Branstetter, Ryan; Borca, Manuel V.] ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. [O'Donnell, Vivian; Risatti, Guillermo R.] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Lu, Zhigiang] DHS, Plum Isl Anim Dis Ctr, Greenport, NY USA. [Largo, Eneko; Nieva, Jose L.] Univ Basque Country, UPV EHU, Biophys Unit, CSIC, Bilbao, Spain. [Largo, Eneko; Nieva, Jose L.] Univ Basque Country, Dept Biochem & Mol Biol, UPV EHU, Bilbao, Spain. [Ambroggio, Xavier] NIAID, Bioinformat & Computat Biosci Branch, NIH, Bethesda, MD 20892 USA. [Carrillo, Consuelo] USDA, Plum Isl Anim Dis Ctr, APHIS, Greenport, NY USA. RP Borca, MV (reprint author), ARS, Plum Isl Anim Dis Ctr, USDA, Greenport, NY USA. EM manuel.borca@ars.usda.gov RI Fernandez Sainz, Ignacio/I-3046-2016; OI Gladue, Douglas/0000-0002-7894-0233; Borca, Manuel/0000-0002-0888-1178 FU Spanish MCINN; Basque Government; University of the Basque Country [BIO2011-29792, GIU 06/42] FX This research was partially supported by Spanish MCINN, Basque Government, and University of the Basque Country grants (BIO2011-29792 and GIU 06/42 to J.L.N.). NR 40 TC 23 Z9 24 U1 0 U2 12 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD JUN PY 2012 VL 86 IS 12 BP 6778 EP 6791 DI 10.1128/JVI.00560-12 PG 14 WC Virology SC Virology GA 953SS UT WOS:000304894100037 PM 22496228 ER PT J AU Kitikoon, P Vincent, AL Gauger, PC Schlink, SN Bayles, DO Gramer, MR Darnell, D Webby, RJ Lager, KM Swenson, SL Klimov, A AF Kitikoon, Pravina Vincent, Amy L. Gauger, Phillip C. Schlink, Sarah N. Bayles, Darrell O. Gramer, Marie R. Darnell, Daniel Webby, Richard J. Lager, Kelly M. Swenson, Sabrina L. Klimov, Alexander TI Pathogenicity and Transmission in Pigs of the Novel A(H3N2)v Influenza Virus Isolated from Humans and Characterization of Swine H3N2 Viruses Isolated in 2010-2011 SO JOURNAL OF VIROLOGY LA English DT Article ID PANDEMIC H1N1 2009; LINKED-IMMUNOSORBENT-ASSAY; A VIRUSES; UNITED-STATES; REASSORTMENT; ANTIBODY; IDENTIFICATION; HEMAGGLUTININ; ARGENTINA; EMERGENCE AB Swine influenza virus (SIV) H3N2 with triple reassorted internal genes (TRIG) has been enzootic in Unites States since 1998. Transmission of the 2009 pandemic H1N1 (pH1N1) virus to pigs in the United States was followed by reassortment with endemic Sly, resulting in reassorted viruses that include novel H3N2 genotypes (rH3N2p). Between July and December 2011, 12 cases of human infections with swine-lineage H3N2 viruses containing the pandemic matrix (pM) gene [A(H3N2)v] were detected. Whole-genome analysis of H3N2 viruses isolated from pigs from 2009 to 2011 sequenced in this study and other available H3N2 sequences showed six different rH3N2p genotypes present in the U.S. swine population since 2009. The presence of the pM gene was a common feature among all rH3N2p genotypes, but no specific genotype appeared to predominate in the swine population. We compared the pathogenic, transmission, genetic, and antigenic properties of a human A(H3N2)v isolate and two swine H3N2 isolates, H3N2-TRIG and rH3N2p. Our in vivo study detected no increased virulence in A(H3N2)v or rH3N2p viruses compared to endemic H3N2-TRIG virus. Antibodies to cluster IV H3N2-TRIG and rH3N2p viruses had reduced cross-reactivity to A(H3N2)v compared to other cluster IV H3N2-TRIG and rH3N2p viruses. Genetic analysis of the hemagglutinin gene indicated that although rH3N2p and A(H3N2)v are related to cluster IV of H3N2-TRIG, some recent rH3N2p isolates appeared to be forming a separate cluster along with the human isolates of A(H3N2)v. Continued monitoring of these H3N2 viruses is necessary to evaluate the evolution and potential loss of population immunity in swine and humans. C1 [Kitikoon, Pravina; Vincent, Amy L.; Gauger, Phillip C.; Schlink, Sarah N.; Lager, Kelly M.] ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Gauger, Phillip C.] Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Coll Vet Med, Ames, IA USA. [Bayles, Darrell O.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Gramer, Marie R.] Univ Minnesota, Coll Vet Med, Vet Diagnost Lab, St Paul, MN 55108 USA. [Darnell, Daniel; Webby, Richard J.] St Jude Childrens Hosp, Dept Infect Dis, Memphis, TN 38105 USA. [Swenson, Sabrina L.] USDA, Natl Vet Serv Labs, APHIS, Ames, IA 50010 USA. [Klimov, Alexander] Ctr Dis Control & Prevent, Influenza Div, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USA. RP Vincent, AL (reprint author), ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. EM amy.vincent@ars.usda.gov FU USDA-ARS; USDA-APHIS-VS; DHHS-CDC FX Funding was provided from USDA-ARS, USDA-APHIS-VS, and an interagency agreement with DHHS-CDC. NR 49 TC 37 Z9 38 U1 1 U2 3 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD JUN PY 2012 VL 86 IS 12 BP 6804 EP 6814 DI 10.1128/JVI.00197-12 PG 11 WC Virology SC Virology GA 953SS UT WOS:000304894100039 PM 22491461 ER PT J AU Palti, Y Genet, C Gao, GT Hu, YQ You, FM Boussaha, M Rexroad, CE Luo, MC AF Palti, Yniv Genet, Carine Gao, Guangtu Hu, Yuqin You, Frank M. Boussaha, Mekki Rexroad, Caird E., III Luo, Ming-Cheng TI A Second Generation Integrated Map of the Rainbow Trout (Oncorhynchus mykiss) Genome: Analysis of Conserved Synteny with Model Fish Genomes SO MARINE BIOTECHNOLOGY LA English DT Article DE Oncorhynchus mykiss; Physical map; Genetic map; Comparative genomics; Conserved synteny ID QUANTITATIVE TRAIT LOCI; BAC-END SEQUENCES; MULTILOCUS LINKAGE ANALYSIS; ATLANTIC SALMON; CHROMOSOME ARMS; PHYSICAL MAP; GENERATION; QTL; CONSTRUCTION; RESISTANCE AB DNA fingerprints and end sequences from bacterial artificial chromosomes (BACs) from two new libraries were generated to improve the first generation integrated physical and genetic map of the rainbow trout (Oncorhynchus mykiss) genome. The current version of the physical map is composed of 167,989 clones of which 158,670 are assembled into contigs and 9,319 are singletons. The number of contigs was reduced from 4,173 to 3,220. End sequencing of clones from the new libraries generated a total of 11,958 high quality sequence reads. The end sequences were used to develop 238 new micro-satellites of which 42 were added to the genetic map. Conserved synteny between the rainbow trout genome and model fish genomes was analyzed using 188,443 BAC end sequence (BES) reads. The fractions of BES reads with significant BLASTN hits against the zebrafish, medaka, and stickleback genomes were 8.8%, 9.7%, and 10.5%, respectively, while the fractions of significant BLASTX hits against the zebrafish, medaka, and stickleback protein databases were 6.2%, 5.8%, and 5.5%, respectively. The overall number of unique regions of conserved synteny identified through grouping of the rainbow trout BES into fingerprinting contigs was 2,259, 2,229, and 2,203 for stickleback, medaka, and zebrafish, respectively. These numbers are approximately three to five times greater than those we have previously identified using BAC paired ends. Clustering of the conserved synteny analysis results by linkage groups as derived from the integrated physical and genetic map revealed that despite the low sequence homology, large blocks of macrosynteny are conserved between chromosome arms of rainbow trout and the model fish species. C1 [Palti, Yniv; Gao, Guangtu; Rexroad, Caird E., III] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. [Genet, Carine; Boussaha, Mekki] INRA, UMR1313, F-78352 Jouy En Josas, France. [Hu, Yuqin; You, Frank M.; Luo, Ming-Cheng] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Palti, Y (reprint author), ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM yniv.palti@ars.usda.gov RI Gao, Guangtu/F-4541-2012; Luo, Ming-Cheng/C-5600-2011 FU National Research Initiative [2007-35616-17875]; USDA National Institute of Food and Agriculture; Agricultural Research Service [1930-31000-009] FX This project was supported by National Research Initiative Competitive Grant number 2007-35616-17875 from the USDA National Institute of Food and Agriculture and by internal base funds provided by the Agricultural Research Service project number 1930-31000-009. Brian Smith, Kristy Shewbridge, and Roseanna Long carried out the microsatellites genotyping described in this manuscript. We thank Gary Thorgaard and Paul Wheeler (Washington State University) for the Swanson doubled haploid samples that were used to construct the new BAC libraries and Eric Peatman (Auburn University) for reviewing an earlier draft of the manuscript. We also thank anonymous reviewer 2 for his insightful comments on the blocks of conserved macro-synteny that can be identified through the chromosomes of the integrated map. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 74 TC 26 Z9 27 U1 1 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1436-2228 J9 MAR BIOTECHNOL JI Mar. Biotechnol. PD JUN PY 2012 VL 14 IS 3 BP 343 EP 357 DI 10.1007/s10126-011-9418-z PG 15 WC Biotechnology & Applied Microbiology; Marine & Freshwater Biology SC Biotechnology & Applied Microbiology; Marine & Freshwater Biology GA 958FL UT WOS:000305221800010 PM 22101344 ER PT J AU Wang, Q Fleisher, DH Timlin, D Reddy, VR Chun, JA AF Wang, Q. Fleisher, D. H. Timlin, D. Reddy, V. R. Chun, J. A. TI Quantifying the measurement errors in a portable open gas-exchange system and their effects on the parameterization of Farquhar et al. model for C-3 leaves SO PHOTOSYNTHETICA LA English DT Article DE open gas-exchange system; measurement errors; parameterization; leaf model; photosynthesis; transpiration ID ATMOSPHERIC CO2 CONCENTRATION; AFFECT LEAF RESPIRATION; TEMPERATURE RESPONSE; CARBON-DIOXIDE; PHOTOSYNTHESIS; DIFFUSION; CHAMBERS AB A portable open gas-exchange system (Li-6400, Li-Cor, Inc., Lincoln, NE, USA) has been widely used for the measurement of net gas exchanges and calibration/parameterization of leaf models. Measurement errors due to diffusive leakage rates of water vapor (L-W) and CO2 (L-C) between inside and outside of the leaf chamber, and the inward dark transpiration rate (D-W) and dark respiration rate (D-C) released from the leaf under the gasket, can be significant. Rigorous model-based approaches were developed for estimating leakage coefficients of water vapor (K-W) and CO2 (K-C) and correcting for the combination of these errors. Models were based on mass balance equations and the Dusty Gas Model for a ternary gas mixture of water vapor, CO2, and dry air. Experiments were conducted using two Li-6400 systems with potato and soybean leaves. Results indicated that models were reliable for estimating K-W and K-C, and the values varied with instrument, chamber size, gasket condition, and leaf structure. A thermally killed leaf should be used for this determination. Measurement error effects on parameterization of the Farquhar et al. (1980) model as determined by P-N/C-i curves were substantial and each parameter had its own sensitivity to measurement errors. Results also indicated that all four error sources should be accounted for when correcting measurements. C1 [Fleisher, D. H.; Timlin, D.; Reddy, V. R.; Chun, J. A.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Wang, Q.] Univ Maryland, Wye Res & Educ Ctr, Queenstown, MD 21658 USA. RP Fleisher, DH (reprint author), USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. EM david.fleisher@ars.usda.gov NR 23 TC 4 Z9 4 U1 3 U2 35 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0300-3604 J9 PHOTOSYNTHETICA JI Photosynthetica PD JUN PY 2012 VL 50 IS 2 BP 223 EP 238 DI 10.1007/s11099-012-0012-z PG 16 WC Plant Sciences SC Plant Sciences GA 958FS UT WOS:000305222500008 ER PT J AU Hilker, T Hall, FG Tucker, CJ Coops, NC Black, TA Nichol, CJ Sellers, PJ Barr, A Hollinger, DY Munger, JW AF Hilker, Thomas Hall, Forrest G. Tucker, Compton J. Coops, Nicholas C. Black, T. Andrew Nichol, Caroline J. Sellers, Piers J. Barr, Alan Hollinger, David Y. Munger, J. W. TI Data assimilation of photosynthetic light-use efficiency using multi-angular satellite data: II Model implementation and validation SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Data assimilation; Photosynthesis; Eddy-flux; Multivariate function; Epsilon; Epsilon max; Global carbon cycle; Carbon modeling; Vegetation carbon cycle; Downregulation; CHRIS/Proba; PRI'; Multi-angular ID CARBON-DIOXIDE FLUXES; GROSS PRIMARY PRODUCTION; DOUGLAS-FIR FOREST; LEAF-AREA INDEX; DECIDUOUS FOREST; BOREAL; MODIS; CANOPY; CANADA; VARIABILITY AB Spatially explicit and temporally continuous estimates of photosynthesis will be of great importance for increasing our understanding of and ultimately closing the terrestrial carbon cycle. Current capabilities to model photosynthesis, however, are limited by accurate enough representations of the complexity of the underlying biochemical processes and the numerous environmental constraints imposed upon plant primary production. A potentially powerful alternative to model photosynthesis through these indirect observations is the use of multi-angular satellite data to infer light-use efficiency (epsilon) directly from spectral reflectance properties in connection with canopy shadow fractions. Hall et al. (this issue) introduced a new approach for predicting gross ecosystem production that would allow the use of such observations in a data assimilation mode to obtain spatially explicit variations in epsilon from infrequent polar-orbiting satellite observations, while meteorological data are used to account for the more dynamic responses of epsilon to variations in environmental conditions caused by changes in weather and illumination. In this second part of the study we implement and validate the approach of Hall et al. (this issue) across an ecologically diverse array of eight flux-tower sites in North America using data acquired from the Compact High Resolution Imaging Spectroradiometer (CHRIS) and eddy-flux observations. Our results show significantly enhanced estimates of epsilon and therefore cumulative gross ecosystem production (GEP) over the course of one year at all examined sites. We also demonstrate that epsilon is greatly heterogeneous even across small study areas. Data assimilation and direct inference of GEP from space using a new, proposed sensor could therefore be a significant step towards closing the terrestrial carbon cycle. (c) 2012 Elsevier Inc. All rights reserved. C1 [Hilker, Thomas; Hall, Forrest G.; Tucker, Compton J.; Sellers, Piers J.] NASA, Goddard Space Flight Ctr, Biospher Sci Branch, Greenbelt, MD 20771 USA. [Coops, Nicholas C.] Univ British Columbia, Fac Forest Resources Management, Vancouver, BC V6T 1Z4, Canada. [Black, T. Andrew] Univ British Columbia, Fac Land & Food Syst, Vancouver, BC V6T 1Z4, Canada. [Nichol, Caroline J.] Univ Edinburgh, Sch Geosci, Edinburgh EH9 3JN, Midlothian, Scotland. [Barr, Alan] Environm Canada, Saskatoon, SK, Canada. [Hollinger, David Y.] US Forest Serv, No Res Stn, Durham, NH USA. [Munger, J. W.] Harvard Univ, Cambridge, MA 02138 USA. RP Hilker, T (reprint author), NASA, Goddard Space Flight Ctr, Biospher Sci Branch, Code 618, Greenbelt, MD 20771 USA. EM thomas.hilker@nasa.gov RI Coops, Nicholas/J-1543-2012; Hollinger, David/G-7185-2012; Barr, Alan/H-9939-2014; Munger, J/H-4502-2013 OI Coops, Nicholas/0000-0002-0151-9037; Munger, J/0000-0002-1042-8452 FU NASA; Canadian Carbon Program; Natural Sciences and Engineering Research Council of Canada (NSERC); BIOCAP FX The ESA CHRIS/Proba images were provided by Dr. David G. Goodenough, Dr. Ray Merton, and Dr. Mathias Kneubuhler, all principal investigators of the Evaluation and Validation of CHRIS (EVC) Project. The Center for Remote Sensing and Department of Geography at Boston University are thanked for provision of the GOMS model. Partial funding for this study was provided by NASA's Terrestrial Ecology Program managed by Dr. Diane Wickland. This research is also partially funded by the Canadian Carbon Program, the Natural Sciences and Engineering Research Council of Canada (NSERC) and BIOCAP, and an NSERC-Accelerator grant to NCC. NR 48 TC 19 Z9 21 U1 3 U2 40 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN PY 2012 VL 121 BP 287 EP 300 DI 10.1016/j.rse.2012.02.008 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 955WN UT WOS:000305051700025 ER PT J AU Colliander, A Chan, S Kim, SB Das, N Yueh, S Cosh, M Bindlish, R Jackson, T Njoku, E AF Colliander, Andreas Chan, Steven Kim, Seung-bum Das, Narendra Yueh, Simon Cosh, Michael Bindlish, Rajat Jackson, Thomas Njoku, Eni TI Long term analysis of PALS soil moisture campaign measurements for global soil moisture algorithm development SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Soil moisture; SMAP (Soil Moisture Active and Passive); SGP99; SMEX02; CLASIC; SMAPVEX08; PALS ID MICROWAVE DIELECTRIC BEHAVIOR; L-BAND RADIOMETER; SURFACE-ROUGHNESS; OCEAN SURFACE; WET SOIL; RETRIEVAL; MODEL; SMOS; EMISSION; MISSION AB An important component of satellite-based soil moisture algorithm development and validation is the comparison of coincident remote sensing and in situ observations that are typically provided by intensive field campaigns. The planned NASA Soil Moisture Active Passive (SMAP) mission has unique requirements compared to previous soil moisture satellite programs because both active and passive microwave observations are needed. The primary source of these combined observations has been an aircraft-based SMAP simulator called PALS (Passive and Active L-band System). This paper presents an overview of the field experiment data collected using PALS that spans 10 years. Data from the various campaigns were merged to form a single data set. Analyses showed that the data set contains an extensive range of soil moisture values collected under a variety of conditions and that the quality of both the PALS and ground truth data meets the needs of SMAP algorithm development and validation. The study suggests that the data set should be expanded in order to achieve globally representative land cover diversity and that more observations under dense vegetation conditions and longer time series would be desirable. (c) 2012 Elsevier Inc. All rights reserved. C1 [Colliander, Andreas; Chan, Steven; Kim, Seung-bum; Das, Narendra; Yueh, Simon; Njoku, Eni] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. [Cosh, Michael; Bindlish, Rajat; Jackson, Thomas] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Colliander, A (reprint author), CALTECH, Jet Prop Lab, 4800 Oak Grove Dr, Pasadena, CA 91109 USA. EM andreas.colliander@jpl.nasa.gov RI Cosh, MIchael/A-8858-2015 OI Cosh, MIchael/0000-0003-4776-1918 NR 68 TC 18 Z9 18 U1 1 U2 21 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN PY 2012 VL 121 BP 309 EP 322 DI 10.1016/j.rse.2012.02.002 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 955WN UT WOS:000305051700027 ER PT J AU Gitelson, AA Peng, Y Masek, JG Rundquist, DC Verma, S Suyker, A Baker, JM Hatfield, JL Meyers, T AF Gitelson, Anatoly A. Peng, Yi Masek, Jeffery G. Rundquist, Donald C. Verma, Shashi Suyker, Andrew Baker, John M. Hatfield, Jerry L. Meyers, Tilden TI Remote estimation of crop gross primary production with Landsat data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Gross primary production; Landsat; Chlorophyll content; Vegetation index; Potential incident photosynthetically active radiation ID CANOPY CHLOROPHYLL CONTENT; LIGHT-USE-EFFICIENCY; LEAF-AREA INDEX; RADIATIVE-TRANSFER; ATMOSPHERIC CORRECTION; INTERANNUAL VARIATION; VEGETATION INDEXES; TERRESTRIAL GROSS; MAIZE; CARBON AB An accurate and synoptic quantification of gross primary production (GPP) in crops is essential for studies of carbon budgets at regional and global scales. In this study, we tested a model, relating crop GPP to a product of total canopy chlorophyll (Chl) content and potential incident photosynthetically active radiation (PAR(potential)). The approach is based on remotely sensed data; specifically, vegetation indices (VI) that are proxies for total Chl content and PAR(potential), which is incident PAR under a condition of minimal atmospheric aerosol loading. Using VI retrieved from surface reflectance Landsat data, we found that the model is capable of accurately estimating GPP in maize, with coefficient of variation (CV) below 23%, and in soybean with CV below 30%. The algorithms established and calibrated over three Mead, Nebraska AmeriFlux sites were able to estimate maize and soybean GPP at tower flux sites in Minnesota, Iowa and Illinois with acceptable accuracy. (c) 2012 Elsevier Inc. All rights reserved. C1 [Gitelson, Anatoly A.; Peng, Yi; Rundquist, Donald C.; Verma, Shashi; Suyker, Andrew] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68588 USA. [Masek, Jeffery G.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Baker, John M.] USDA, Soil & Water Management Res Unit, Minneapolis, MN USA. [Hatfield, Jerry L.] USDA, Natl Lab Agr & Environm, Ames, IA USA. [Meyers, Tilden] Natl Ocean & Atmospher Adm, Oak Ridge, TN USA. RP Gitelson, AA (reprint author), Univ Nebraska, Sch Nat Resources, Lincoln, NE 68588 USA. EM agitelson2@unl.edu RI Gitelson, Anatoly/G-3452-2012; Masek, Jeffrey/D-7673-2012; Meyers, Tilden/C-6633-2016 FU NASA NACP [NNX08AI75G]; U.S. Department of Energy [DE-FG-02-00ER45827]; Office of Science (BER) [DE-FG03-00ER62996]; Center for Advanced Land Management Information Technologies (CALMIT); University of Nebraska-Lincoln FX This research was supported by NASA NACP grant no. NNX08AI75G and partially by the U.S. Department of Energy: (a) EPSCoR program, grant no. DE-FG-02-00ER45827 and (b) Office of Science (BER), grant no. DE-FG03-00ER62996. We sincerely appreciate the support and the use of facilities and equipment provided by the Center for Advanced Land Management Information Technologies (CALMIT) and Carbon Sequestration Program, University of Nebraska-Lincoln. NR 63 TC 59 Z9 64 U1 6 U2 77 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN PY 2012 VL 121 BP 404 EP 414 DI 10.1016/j.rse.2012.02.017 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 955WN UT WOS:000305051700034 ER PT J AU Wegrzyn, JL Main, D Figueroa, B Choi, M Yu, J Neale, DB Jung, S Lee, T Stanton, M Zheng, P Ficklin, S Cho, I Peace, C Evans, K Volk, G Oraguzie, N Chen, C Olmstead, M Gmitter, G Abbott, AG AF Wegrzyn, J. L. Main, D. Figueroa, B. Choi, M. Yu, J. Neale, D. B. Jung, S. Lee, T. Stanton, M. Zheng, P. Ficklin, S. Cho, I. Peace, C. Evans, K. Volk, G. Oraguzie, N. Chen, C. Olmstead, M. Gmitter, G., Jr. Abbott, A. G. TI Uniform standards for genome databases in forest and fruit trees SO TREE GENETICS & GENOMES LA English DT Review DE Phenotype; Ontologies; Genome database; Genotype; Web services; Genome annotation ID TOOL AB TreeGenes and tree fruit Genome Database Resources serve the international forestry and fruit tree genomics research communities, respectively. These databases hold similar sequence data and provide resources for the submission and recovery of this information in order to enable comparative genomics research. Large-scale genotype and phenotype projects have recently spawned the development of independent tools and interfaces within these repositories to deliver information to both geneticists and breeders. The increase in next generation sequencing projects has increased the amount of data as well as the scale of analysis that can be performed. These two repositories are now working towards a similar goal of archiving the diverse, independent data sets generated from genotype/phenotype experiments. This is achieved through focused development on data input standards (templates), pipelines for the storage and automated curation, and consistent annotation efforts through the application of widely accepted ontologies to improve the extraction and exchange of the data for comparative analysis. Efforts towards standardization are not limited to genotype/phenotype experiments but are also being applied to other data types to improve gene prediction and annotation for de novo sequencing projects. The resources developed towards these goals represent the first large-scale coordinated effort in plant databases to add informatics value to diverse genotype/phenotype experiments. C1 [Wegrzyn, J. L.; Figueroa, B.; Choi, M.; Yu, J.; Neale, D. B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Main, D.; Jung, S.; Lee, T.; Zheng, P.; Ficklin, S.; Peace, C.; Evans, K.; Oraguzie, N.] Washington State Univ, Dept Hort & Landscape Architecture, Pullman, WA 99164 USA. [Cho, I.] Saginaw Valley State Univ, Dept Comp Sci, Saginaw, MI 48710 USA. [Stanton, M.] Clemson Univ, Genom Inst, Clemson, SC 29634 USA. [Volk, G.] ARS, Natl Ctr Genet Resources Preservat, USDA, Ft Collins, CO 80521 USA. [Olmstead, M.] Univ Florida, IFAS, Dept Hort Sci, Gainesville, FL 32611 USA. [Chen, C.; Gmitter, G., Jr.] Univ Florida, Ctr Agr Res & Educ, Lake Alfred, FL 33850 USA. [Abbott, A. G.] Clemson Univ, Dept Biochem & Genet, Clemson, SC 29634 USA. RP Wegrzyn, JL (reprint author), Univ Calif Davis, Dept Plant Sci, Mail Stop 4, Davis, CA 95616 USA. EM jlwegrzyn@ucdavis.edu; dorrie@wsu.edu OI Ficklin, Stephen/0000-0001-9138-6292; Wegrzyn, Jill/0000-0001-5923-0888 NR 15 TC 7 Z9 7 U1 0 U2 15 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD JUN PY 2012 VL 8 IS 3 BP 549 EP 557 DI 10.1007/s11295-012-0494-7 PG 9 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA 958MJ UT WOS:000305241900006 ER PT J AU Beati, L Patel, J Lucas-Williams, H Adakal, H Kanduma, EG Tembo-Mwase, E Krecek, R Mertins, JW Alfred, JT Kelly, S Kelly, P AF Beati, Lorenza Patel, Jaymin Lucas-Williams, Helene Adakal, Hassane Kanduma, Esther G. Tembo-Mwase, Enala Krecek, Rosina Mertins, James W. Alfred, Jeffery T. Kelly, Susyn Kelly, Patrick TI Phylogeography and Demographic History of Amblyomma variegatum (Fabricius) (Acari: Ixodidae), the Tropical Bont Tick SO VECTOR-BORNE AND ZOONOTIC DISEASES LA English DT Article DE Amblyomma variegatum; Population genetics ID EHRLICHIA-RUMINANTIUM; POPULATION-GENETICS; IXODES-SCAPULARIS; SYSTEMATIC RELATIONSHIPS; COWDRIA-RUMINANTIUM; RICKETTSIA-AFRICAE; CARIBBEAN ISLANDS; FRENCH-ANTILLES; HEARTWATER; CATTLE AB The genetic diversity of Amblyomma variegatum (Fabricius) from four Caribbean islands and five African countries was compared by analyzing the sequences of three gene fragments, two mitochondrial (12SrDNA and D-Loop-DL), and one nuclear (intergenic transcribed spacer 2 [ITS2]). Genetic variability of the ITS2 DNA fragment consisted of only uninformative single nucleotide mutations, and therefore this gene was excluded from further analyses. Mitochondrial gene divergences among African populations and between Caribbean and African populations were very low. Nevertheless, the data suggest that A. variegatum is divided into distinct East and West African groups, the western group including all Caribbean samples. Phylogenetic analyses of the 12SrDNA and DL gene sequences showed that the West African A. variegatum clustered in a well-supported monophyletic clade, distinct from eastern paraphyletic lineages. Sequences of A. variegatum from the Caribbean were embedded in the West African clade, which supports the known West African historical origin for these ticks. C1 [Beati, Lorenza; Patel, Jaymin] Georgia So Univ, Inst Arthropodol & Parasitol, Statesboro, GA 30460 USA. [Lucas-Williams, Helene; Krecek, Rosina; Kelly, Susyn; Kelly, Patrick] Ross Univ, Sch Vet Med, Basseterre, St Kitts & Nevi. [Adakal, Hassane] CIRDES, Bobo Dioulasso, Burkina Faso. [Kanduma, Esther G.] Univ Nairobi, Dept Biochem, Nairobi, Kenya. [Kanduma, Esther G.] ILRI, Biosci Eastern & Cent Africa Hub, Nairobi, Kenya. [Tembo-Mwase, Enala] Univ Zambia, Sch Vet Med, Lusaka, Zambia. [Krecek, Rosina] Univ Johannesburg, Dept Zool, Auckland Pk, South Africa. [Mertins, James W.; Alfred, Jeffery T.] US Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Natl Vet Serv Labs, Ames, IA USA. RP Beati, L (reprint author), Georgia So Univ, Inst Arthropodol & Parasitol, 75 Georgia Ave,Bldg 204,POB 8056, Statesboro, GA 30460 USA. EM lorenzabeati@georgiasouthern.edu FU Ross University School of Veterinary Medicine Intramural Research; NSF [1026146] FX This study was supported through a Ross University School of Veterinary Medicine Intramural Research Grant, and in part by NSF grant #1026146 to L. Beati. NR 48 TC 21 Z9 21 U1 1 U2 22 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1530-3667 J9 VECTOR-BORNE ZOONOT JI Vector-Borne Zoonotic Dis. PD JUN PY 2012 VL 12 IS 6 BP 514 EP 525 DI 10.1089/vbz.2011.0859 PG 12 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA 957DR UT WOS:000305139400011 PM 22448720 ER PT J AU Bierman, PM Rosen, CJ Venterea, RT Lamb, JA AF Bierman, Peter M. Rosen, Carl J. Venterea, Rodney T. Lamb, John A. TI Survey of nitrogen fertilizer use on corn in Minnesota SO AGRICULTURAL SYSTEMS LA English DT Article DE N fertilizer; N management; Fertilizer N recovery; N application rates; N application timing; Nitrification inhibitors AB A survey was conducted in the spring of 2010 to characterize the use of nitrogen (N) fertilizer on corn (Zea mays L.) by Minnesota farmers in the 2009 growing season. Detailed information on synthetic N fertilizer management practices was collected from interviews with 1496 farmers distributed across all of the corn growing regions in the state. The total amount of corn they grew represented 6.8% of the ha of corn harvested in Minnesota in 2009. This report summarizes data on: (1) N fertilizer rates, (2) major N sources (excluding manures), (3) application timing of the major N source, (4) use of nitrification inhibitors, additives, and specialty N fertilizer formulations, (5) fertilizer placement and incorporation practices, (6) use of starter fertilizer, split and sidedress applications, and other N sources such as ammonium phosphates, (7) N fertilization of irrigated corn, and (8) use of soil testing as a fertility management tool. Many of the survey results are reported as statewide averages, but where regional differences occurred the data are broken down and presented separately for different parts of the state. This survey provides the most comprehensive set of data on N fertilizer use on corn that has been collected in Minnesota. The information can be used to target research and education programs to improve N management for both production and environmental goals. The statewide average N fertilizer rate was 157 kg N ha(-1). Variable rate application was used to apply N by 23% of farmers. About 59% of surveyed farmers applied the majority of their N fertilizer in the spring before planting, 32.5% made their main N application in the fall, and 9% side-dressed the majority of their N after corn emergence. Most farmers used anhydrous ammonia (46%) or urea (45%) as their major source of N fertilizer, while 6.5% used a liquid N formulation as their primary N source. Soil testing was used as a fertility management tool on 84% of the surveyed fields in the last 5 years. Overall results indicate that N fertilizer use by Minnesota corn farmers is generally consistent with University of Minnesota Extension N management guidelines. Fertilizer N use could probably be improved by taking adequate N credit for previous soybean crops. In the South Central region of the state, fertilizer N recovery could potentially be improved by increased use of nitrification inhibitors with fall-applied anhydrous ammonia or by delaying anhydrous ammonia application until spring. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Bierman, Peter M.; Rosen, Carl J.; Venterea, Rodney T.; Lamb, John A.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Venterea, Rodney T.] USDA ARS, St Paul, MN 55108 USA. RP Rosen, CJ (reprint author), Univ Minnesota, Dept Soil Water & Climate, 439 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. EM crosen@umn.edu RI Venterea, Rodney/A-3930-2009; Rosen, Carl/L-2622-2013 OI Rosen, Carl/0000-0002-0098-2230 FU National Research Initiative from the USDA CSREES/NIFA [2009-55112-05216]; Minnesota Department of Agriculture (MDA) FX Funding for this Project was provided by: (1) National Research Initiative Competitive Grant No. 2009-55112-05216 from the USDA CSREES/NIFA Air Quality Program, and (2) The Minnesota Department of Agriculture (MDA) using money provided by the Clean Water Fund (from the Clean Water, Land and Legacy Amendment). Denton Breuning of MDA provided support processing and organizing survey data. The survey was conducted by the USDA National Agricultural Statistics Service (NASS), Minnesota Field Office. NR 12 TC 14 Z9 15 U1 3 U2 45 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-521X J9 AGR SYST JI Agric. Syst. PD JUN PY 2012 VL 109 BP 43 EP 52 DI 10.1016/j.agsy.2012.02.004 PG 10 WC Agriculture, Multidisciplinary SC Agriculture GA 951RR UT WOS:000304738200006 ER PT J AU Yilma, S Vales, MI Charlton, BA Hane, DC James, SR Shock, CC Mosley, AR Culp, D Feibert, E Leroux, L Karaagac, E Knowles, NR Pavek, MJ Stark, JC Novy, RG Whitworth, JL Pavek, JJ Corsini, DL Brandt, TL Olsen, N Brown, CR AF Yilma, S. Vales, M. I. Charlton, B. A. Hane, D. C. James, S. R. Shock, C. C. Mosley, A. R. Culp, D. Feibert, E. Leroux, L. Karaagac, E. Knowles, N. R. Pavek, M. J. Stark, J. C. Novy, R. G. Whitworth, J. L. Pavek, J. J. Corsini, D. L. Brandt, T. L. Olsen, N. Brown, C. R. TI Owyhee Russet: A Variety with High Yields of U.S. No. 1 Tubers, Excellent Processing Quality, and Moderate Resistance to Fusarium Dry Rot (Fusarium solani var. coeruleum) SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Breeding; Variety; Processing; Common scab resistance; Fusarium dry rot resistance ID POTATO GERMPLASM AB Owyhee Russet (AO96160-3) originated from a cross between A89384-10 and A89512-3 in 1996. Owyhee Russet was released in 2009 by Oregon State University, in cooperation with the USDA-ARS and the Agricultural Experiment Stations of Idaho and Washington and is a product of the Northwest Potato Variety (Tri-State) Development Program. Owyhee Russet has semi-erect medium sized vines with medium to late maturity. The tubers are long, with a tan skin, medium russeting, and attractive tuber appearance for fresh market. Owyhee Russet was evaluated in several locations across the Northwest for more than 15 years. Total yield of Owyhee Russet is similar to that of Russet Burbank and Ranger Russet but significantly higher than Russet Norkotah. U.S. No.1 tuber yield of Owyhee Russet is significantly higher than Russet Burbank and Russet Norkotah, resulting in substantially higher marketable yield. Owyhee Russet tubers have significantly higher specific gravity than Russet Burbank and Russet Norkotah. Fry color following tuber storage at 4A degrees C and 9A degrees C is significantly lighter for Owyhee Russet than the comparison varieties. Relative strengths include high yield with a very high proportion of U.S. No.1 tubers, good tuber appearance and excellent processing quality, resistance to cold sweetening, common scab and Fusarium dry rot. Weaknesses include susceptibility to foliar and tuber late blight and susceptibility to metribuzin herbicide injury. Allelic patterns of five SSR markers have shown that Owyhee Russet has a distinctive DNA genetic fingerprint from its russet type reference varieties which are Ranger Russet, Russet Burbank, and Russet Norkotah. C1 [Yilma, S.; Hane, D. C.; James, S. R.; Mosley, A. R.; Leroux, L.; Karaagac, E.] Oregon State Univ, Corvallis, OR 97331 USA. [Vales, M. I.] Int Crops Res Inst Semi Arid Trop, Patancheru 502324, Andhra Pradesh, India. [Charlton, B. A.; Culp, D.] Oregon State Univ, Klamath Basin R&E Ctr, Klamath Falls, OR 97603 USA. [Shock, C. C.; Feibert, E.] Oregon State Univ, Malheur Expt Stn, Ontario, OR 97914 USA. [Knowles, N. R.; Pavek, M. J.] Washington State Univ, Pullman, WA 99164 USA. [Stark, J. C.] Univ Idaho, Twin Falls R&E Ctr, Twin Falls, ID 83303 USA. [Novy, R. G.; Whitworth, J. L.] ARS, USDA, Aberdeen Res & Extens R&E Ctr, Aberdeen, ID 83210 USA. [Pavek, J. J.; Corsini, D. L.] ARS, USDA, Aberdeen, ID 83210 USA. [Brandt, T. L.; Olsen, N.] Univ Idaho, Kimberly R&E Ctr, Kimberly, ID 83341 USA. [Brown, C. R.] ARS, USDA, Prosser, WA 99350 USA. RP Yilma, S (reprint author), Oregon State Univ, Corvallis, OR 97331 USA. EM Solomon.Yilma@oregonstate.edu FU Oregon Agricultural Experiment Station; Oregon Potato Commission; Potato Variety Management Institute (PVMI); USDA Cooperative State Research, Education, and Extension Service; USDA Agricultural Research Service FX The authors thank Jenny Reed, Robert Smith, Lori Ewing, Margaret Bain, Nora Fuller, Zach Holden, and Brian Schneider for their contributions to the development and release of Owyhee Russet. We also thank our industry cooperators, our collaborators in the Western Regional Potato Variety Trials, and the Idaho, Washington, and Oregon potato commissions. The Oregon Agricultural Experiment Station, the Oregon Potato Commission, the Potato Variety Management Institute (PVMI), the USDA Cooperative State Research, Education, and Extension Service, and the USDA Agricultural Research Service provided partial financial support for this research. NR 11 TC 1 Z9 1 U1 2 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2012 VL 89 IS 3 BP 175 EP 183 DI 10.1007/s12230-012-9239-2 PG 9 WC Agronomy SC Agriculture GA 952ZT UT WOS:000304836100001 ER PT J AU Haynes, KG Yencho, GC Clough, ME Henninger, MR Sterrett, SB AF Haynes, K. G. Yencho, G. C. Clough, M. E. Henninger, M. R. Sterrett, S. B. TI Genetic Variation for Potato Tuber Micronutrient Content and Implications for Biofortification of Potatoes to Reduce Micronutrient Malnutrition SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Copper; Iron; Manganese; Zinc; Nutrition ID STAPLE FOOD CROPS; IRON-DEFICIENCY; ZINC; HERITABILITY; CULTIVARS; STABILITY; NUTRITION; SELENIUM; VITAMIN; DENSITY AB Micronutrients are crucial to healthy growth and development, yet a large proportion of the world's population suffers from micronutrient deficiencies. Biofortification of staple foods has tremendous potential to alleviate these deficiencies. Potato production in developing countries is increasing rapidly, and therefore, biofortification of potatoes for essential micronutrients may be feasible. The purpose of this study was to determine the amount of genetic variation for micronutrient content in potato germplasm. Eighteen potato clones, consisting of 'Atlantic' and 17 4x-2x hybrids between S. tuberosum and diploid hybrids of S. phureja-S. stenotomum, were grown in three locations (NC, VA, NJ) 2 years (2001, 2002). Samples of tuber tissue were analyzed for copper (Cu), iron (Fe), manganese (Mn) and zinc (Zn). There were significant differences among clones for Cu, Fe, Mn and Zn. Clone x environment interactions were significant for Cu and Zn. Broad-sense heritability and its 95 % confidence interval for Cu was 0.65 (0.50-0.89); Fe was 0.49 (0.27-0.84); Mn was 0.84 (0.82-0.96); and Zn was 0.82 (0.73-0.94). Genetic variation for these four micronutrients is large, suggesting that the micronutrient content of potatoes can be improved through breeding. C1 [Haynes, K. G.] ARS, USDA, Beltsville, MD 20705 USA. [Yencho, G. C.; Clough, M. E.] N Carolina State Univ, Raleigh, NC 27695 USA. [Henninger, M. R.] Rutgers State Univ, New Brunswick, NJ 08903 USA. [Sterrett, S. B.] Virginia Tech, Eastern Shore Agr Res & Extens Ctr, Painter, VA 23420 USA. RP Haynes, KG (reprint author), ARS, USDA, Beltsville, MD 20705 USA. EM kathleen.haynes@ars.usda.gov NR 41 TC 9 Z9 10 U1 1 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X J9 AM J POTATO RES JI Am. J. Potato Res. PD JUN PY 2012 VL 89 IS 3 BP 192 EP 198 DI 10.1007/s12230-012-9242-7 PG 7 WC Agronomy SC Agriculture GA 952ZT UT WOS:000304836100003 ER PT J AU McClendon, SD Mao, ZC Shin, HD Wagschal, K Chen, RR AF McClendon, Shara D. Mao, Zichao Shin, Hyun-Dong Wagschal, Kurt Chen, Rachel R. TI Designer Xylanosomes: Protein Nanostructures for Enhanced Xylan Hydrolysis SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE Xylan hydrolysis; Cellulosome; Xylanosome; Hemicellulases; Lignocelluloses bioprocessing ID CLOSTRIDIUM-THERMOCELLUM CELLULOSOME; QUANTITATIVE PROTEOMIC ANALYSIS; COHESIN-DOCKERIN INTERACTION; ESCHERICHIA-COLI; SACCHAROMYCES-CEREVISIAE; ETHANOL-PRODUCTION; CELL-SURFACE; MINICELLULOSOMES; CELLULOVORANS; DEGRADATION AB This work reports the successful design, construction, and application of multi-functional, self-assembling protein complex, termed xylanosomes. Using the architecture of cellulosomes as template, these structures were designed specifically for hemicellulose hydrolysis. Four different xylanosomes were developed, with up to three different hemicellulase activities combined into a single structure. Each xylanosome was composed of two native or chimeric hemicellulases and tested on wheat arabinoxylan or destarched corn bran for enzymatic hydrolysis. After 24-h incubation, soluble sugars released from arabinoxylan increased up to 30 % with xylanosomes containing a xylanase and bi-functional arabinofuranosidase/xylosidase over the corresponding free, unstructured enzymes. Additionally, xylanosomes with a xylanase and a ferulic acid esterase removed between 15 and 20 % more ferulic acid from wheat arabinoxylan than free enzymes. Furthermore, xylanosomes exhibited synergy with cellulases on destarched corn bran, suggesting a possible use of these nanostructures in cellulose hydrolysis. C1 [McClendon, Shara D.; Mao, Zichao; Shin, Hyun-Dong; Chen, Rachel R.] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA. [Wagschal, Kurt] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Chen, RR (reprint author), Georgia Inst Technol, Sch Chem & Biomol Engn, 311 Ferst Dr NW, Atlanta, GA 30332 USA. EM rchen@chbe.gatech.edu FU USDA-CSREES [2007-02134]; NSF [CBET-0653773]; NSF through University of California, Riverside; UNCFSP/NASA FX This work is supported by grants from USDA-CSREES (2007-02134) and NSF (CBET-0653773, and an NSF grant (through a subcontract from the University of California, Riverside) to RC. SDM acknowledges a graduate fellowship from UNCFSP/NASA. NR 38 TC 9 Z9 9 U1 2 U2 19 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD JUN PY 2012 VL 167 IS 3 BP 395 EP 411 DI 10.1007/s12010-012-9680-1 PG 17 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA 953WL UT WOS:000304904200001 PM 22555497 ER EF