FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Witmer, GW Snow, NP Moulton, RS AF Witmer, Gary W. Snow, Nathan P. Moulton, Rachael S. TI The effects of vitamin K1-rich plant foods on the efficacy of the anticoagulant rodenticides chlorophacinone and diphacinone, used against Montane Voles (Microtus montanus) SO INTERNATIONAL JOURNAL OF PEST MANAGEMENT LA English DT Article DE anticoagulants; California; Microtus; rodenticide; vitamin K; wildlife damage ID ANTI-COAGULANT BAITS; POPULATION-CYCLES; APPLE ORCHARDS; RATS; WASHINGTON; WORLD; MICE AB Voles can cause significant losses to agriculture and wood fibre production. California growers typically rely on baits containing chlorophacinone and diphacinone to reduce vole population densities, but the efficacy of those rodenticides has been decreasing. One hypothesis suggests that voles are consuming high levels of an antidote (vitamin K1) to the anticoagulants, contained within green leafy plants. We tested that hypothesis by first feeding Montane Voles (Microtus montanus) diets that were high in vitamin K1, and then providing the animals with either: (1) chlorophacinone-containing bait, (2) diphacinone-containing bait, or (3) a control diet. We found that the chlorophacinone-containing bait remained efficacious (100% mortality), whereas the diphacinone-containing bait had a much lower efficacy (60% mortality). When only the diphacinone-containing bait was presented, the efficacy was somewhat better (80%). We infer that a diet rich in vitamin K1 did not negate the effects of the chlorophacinone for voles, and so we recommend its continued use in California unless anticoagulant resistance is known to have developed in the vole population. We hypothesise that: (1) diphacinone has a relatively low efficacy against Montane Voles when compared to chlorophacinone, and (2) this lower efficacy could be further reduced by a vitamin K1-rich diet. C1 [Witmer, Gary W.; Snow, Nathan P.; Moulton, Rachael S.] Wildlife Serv, USDA APHIS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Witmer, GW (reprint author), Wildlife Serv, USDA APHIS, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM Gary.w.witmer@aphis.usda.gov FU California Department of Food and Agriculture FX The California Department of Food and Agriculture provided funding for this study. All methods used in this study, QA-1629, were approved under the National Wildlife Research Center's Institutional Animal Care and Use Committee, and hence, is in compliance with the Animal Welfare Act of the United States. Reference to trade names does not imply US government endorsement of commercial products or exclusion of similar products with equal or better effectiveness. NR 42 TC 1 Z9 1 U1 0 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0967-0874 EI 1366-5863 J9 INT J PEST MANAGE JI Int. J. Pest Manage. PD JUL 1 PY 2013 VL 59 IS 3 BP 205 EP 210 DI 10.1080/09670874.2013.816453 PG 6 WC Entomology SC Entomology GA 240CB UT WOS:000326071200006 ER PT J AU Pyke, DA Wirth, TA Beyers, JL AF Pyke, David A. Wirth, Troy A. Beyers, Jan L. TI Does Seeding After Wildfires in Rangelands Reduce Erosion or Invasive Species? SO RESTORATION ECOLOGY LA English DT Article DE aerial seeding; Bromus tectorum; drill seeding; erosion; pinyon-juniper; sagebrush ID CHEATGRASS BROMUS-TECTORUM; WESTERN UNITED-STATES; SAGEBRUSH-STEPPE; PLANT COMMUNITY; BURNED SOILS; WIND EROSION; GREAT-BASIN; FIRE; IMPACTS; REVEGETATION AB Mitigation of ecological damage caused by rangeland wildfires has historically been an issue restricted to the western United States. It has focused on conservation of ecosystem function through reducing soil erosion and spread of invasive plants. Effectiveness of mitigation treatments has been debated recently. We reviewed recent literature to conduct a meta-analysis of seeding after wildfires to determine if seedings may (1) protect ecosystems against soil erosion and (2) reduce invasion or abundance of undesirable nonnative plant species. Effectiveness of postfire seedings was examined in 8 erosion and 19 invasive species cases. Seeding has little effect on erosion during the first year after fire and is highly dependent upon initial establishment and coverage of species in successive years. Among all seeding cases, 28% reduced, 67% were neutral, and 5% increased invasive species abundance. Older seedings were more likely to show reductions in invasives than younger seedings. Seedings with high plant establishment were more likely to reduce invasives than those with low establishment. Studies are needed that examine (1) frequency of adequate establishment of postfire seedings and causal factors of success or failure, (2) long-term impacts of seeding along a range of initial establishment and concomitant plant coverage over time as it relates to erosion and abundance of invasive plant species, and (3) auxiliary treatments designed to increase likelihood of germination and establishment given the inevitable variability of environmental conditions. These studies would aid land managers in deciding when postfire treatments are required and their likely level of success. C1 [Pyke, David A.; Wirth, Troy A.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. [Beyers, Jan L.] US Forest Serv, Pacific Southwest Res Stn, Riverside, CA 92507 USA. RP Pyke, DA (reprint author), US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM david_a_pyke@usgs.gov FU Joint Fire Science Program [08-2-1-13] FX This work was funded by the Joint Fire Science Program (project ID 08-2-1-13). Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 58 TC 16 Z9 16 U1 6 U2 50 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1061-2971 EI 1526-100X J9 RESTOR ECOL JI Restor. Ecol. PD JUL PY 2013 VL 21 IS 4 BP 415 EP 421 DI 10.1111/rec.12021 PG 7 WC Ecology SC Environmental Sciences & Ecology GA 241IA UT WOS:000326158600002 ER PT J AU Windham, WR Yoon, SC Ladely, SR Haley, JA Heitschmidt, JW Lawrence, KC Park, B Narrang, N Cray, WC AF Windham, William R. Yoon, Seung-Chul Ladely, Scott R. Haley, Jennifer A. Heitschmidt, Jerry W. Lawrence, Kurt C. Park, Bosoon Narrang, Neelam Cray, William C. TI Detection by Hyperspectral Imaging of Shiga Toxin-Producing Escherichia coli Serogroups O26, O45, O103, O111, O121, and O145 on Rainbow Agar SO JOURNAL OF FOOD PROTECTION LA English DT Article ID HEMOLYTIC-UREMIC SYNDROME; INFECTIONS; OUTBREAK; DIFFERENTIATION; SPECTRA AB The U.S. Department of Agriculture, Food Safety Inspection Service has determined that six non-O157 Shiga toxin-producing Escherichia call (STEC) serogroups (O26, O45, O103, O111, O121, and O145) are adulterants in raw beef. Isolate and phenotypic discrimination of non-O157 STEC is problematic due to the lack of suitable agar media. The lack of distinct phenotypic color variation among non-O157serogroups cultured on chromogenic agar poses a challenge in selecting colonies for confirmation. In this study, visible and near-infrared hyperspectral imaging and chemometrics were used to detect and classify non-O157 STEC serogroups grown on Rainbow agar O157. The method was first developed by building spectral libraries for each serogroup obtained from ground-truth regions of interest representing the true identity of each pixel and thus each pure culture colony in the hyperspectral agar-plate image. The spectral library for the pure-culture non-O157 STEC consisted of 2,171 colonies, with spectra derived from 124,347 of pixels. The classification models for each serogroup were developed with a k nearest-neighbor classifier. The overall classification training accuracy at the colony level was 99%. The classifier was validated with ground heel enrichments artificially inoculated with 10, 50, and 100 CFU/ml STEC. The validation ground-truth regions of interest of the STEC target colonies consisted of 606 colonies, with 3,030 pixels of spectra. The overall classification accuracy was 98%. The average specificity of the method was 98% due to the low false-positive rate of 1.2%. The sensitivity ranged from 78 to 100% due to the false-negative rates of 22, 7, and 8% for O145, O45, and O26, respectively. This study showed the potential of visible and near-infrared hyperspectral imaging for detecting and classifying colonies of the six non-O157 STEC serogroups. The technique needs to be validated with bacterial cultures directly extracted from meat products and positive identification of colonies by using confirmatory tests such as latex agglutination tests or PCR. C1 [Windham, William R.; Yoon, Seung-Chul; Haley, Jennifer A.; Heitschmidt, Jerry W.; Lawrence, Kurt C.; Park, Bosoon] ARS, Qual & Safety Assessment Res Unit, Richard B Russell Res Ctr, USDA, Athens, GA 30605 USA. [Ladely, Scott R.; Narrang, Neelam; Cray, William C.] Food Safety Inspect Serv, Outbreak Sect, Eastern Lab, USDA, Athens, GA 30605 USA. RP Windham, WR (reprint author), ARS, Qual & Safety Assessment Res Unit, Richard B Russell Res Ctr, USDA, Athens, GA 30605 USA. EM bob.windham@ars.usda.gov NR 32 TC 15 Z9 15 U1 2 U2 21 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2013 VL 76 IS 7 BP 1129 EP 1136 DI 10.4315/0362-028X.JFP-12-497 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FX UT WOS:000326081500002 PM 23834786 ER PT J AU Breidt, F Kay, K Cook, J Osborne, J Ingham, B Arritt, F AF Breidt, F., Jr. Kay, K. Cook, J. Osborne, J. Ingham, B. Arritt, F. TI Determination of 5-Log Reduction Times for Escherichia coli O157:H7, Salmonella enterica, or Listeria monocytogenes in Acidified Foods with pH 3.5 or 3.8 SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ACID RESISTANCE; SURVIVAL AB A critical factor in ensuring the safety of acidified foods is the establishment of a thermal process that assures the destruction of acid-resistant vegetative pathogenic and spoilage bacteria. For acidified foods such as dressings and mayonnaises with pH values of 3.5 or higher, the high water phase acidity (acetic acid of 1.5 to 2.5% or higher) can contribute to lethality, but there is a lack of data showing how the use of common ingredients such as acetic acid and preservatives, alone or in combination, can result in a 5-log reduction for strains of Escherichia coli O157:H7, Salmonella enterica, and Listeria monocytogenes in the absence of a postpackaging pasteurization step. In this study, we determined the times needed at 10 degrees C to achieve a 5-log reduction of E. coli O157:H7, S. enteric:a, and F. monocytogenes in pickling brines with a variety of acetic and benzoic acid combinations at pH 3.5 and 3.8. Evaluation of 15 different acid-pH combinations confirmed that strains of E. coli O157:H7 were significantly more acid resistant than strains of S. enterica and L. monocytogenes. Among the acid conditions tested, holding times of 4 days or less could achieve a 5-log reduction for vegetative pathogens at pH 3.5 with 2.5% acetic acid or at pH 3.8 with 2.5% acetic acid containing 0.1% benzoic acid. These data indicate the efficacy of benzoic acid for reducing the time necessary to achieve a 5-log reduction in target pathogens and may be useful for supporting process filings and the determination of critical controls for the manufacture of acidified foods. C1 [Breidt, F., Jr.] N Carolina State Univ, USDA, ARS, South Atlantic Area Food Sci Res Unit, Raleigh, NC 27695 USA. [Kay, K.; Arritt, F.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27698 USA. [Cook, J.] Gedney Foods Co, Chaska, MN 55318 USA. [Osborne, J.] N Carolina State Univ, Dept Stat, Raleigh, NC 27695 USA. [Ingham, B.] Univ Wisconsin, Dept Food Sci, Madison, WI 53706 USA. RP Breidt, F (reprint author), N Carolina State Univ, USDA, ARS, South Atlantic Area Food Sci Res Unit, 322 Schaub Hall,Box 7624, Raleigh, NC 27695 USA. EM fred.breidt@ars.usda.gov FU Marcatus QED (Toronto, Ontario, Canada); Cargill, Inc. (Minnetonka, MN); Edco Food Products, Inc. (Hobart, WI); U.S. Department of Agriculture (USDA) National Integrated Food Safety Initiative [2011-51110-31019]; Pickle Packers International, Inc. (Washington, DC); USDA Cooperative State Research, Education, and Extension Service project [2006-01240] FX This study was supported in part by research grants from Marcatus QED (Toronto, Ontario, Canada), Cargill, Inc. (Minnetonka, MN), Edco Food Products, Inc. (Hobart, WI), U.S. Department of Agriculture (USDA) National Integrated Food Safety Initiative 2011-51110-31019, Pickle Packers International, Inc. (Washington, DC), and USDA Cooperative State Research, Education, and Extension Service project 2006-01240. We thank Ms. Sandra Parker for excellent editorial assistance. NR 18 TC 3 Z9 3 U1 6 U2 22 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2013 VL 76 IS 7 BP 1245 EP 1249 DI 10.4315/0362-028X.JFP-12-528 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FX UT WOS:000326081500016 PM 23834800 ER PT J AU Edrington, TS Loneragan, GH Hill, J Genovese, KJ He, H Callaway, TR Anderson, RC Brichta-Harhay, DM Nisbet, DJ AF Edrington, T. S. Loneragan, G. H. Hill, J. Genovese, K. J. He, H. Callaway, T. R. Anderson, R. C. Brichta-Harhay, D. M. Nisbet, D. J. TI Development of a Transdermal Salmonella Challenge Model in Calves SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; IN-GROUND BEEF; LYMPH-NODES; CATTLE; PREVALENCE; SLAUGHTER AB :Recent investigations have found that Salmonella can be routinely recovered from peripheral lymph nodes (PLNs) of cattle presented for harvest. When contained within the PLNs, this foodborne pathogen is protected from currently used postharvest, in-plant intervention strategies and, therefore. PLNs harboring Salmonella may be a potential contaminant of ground beef. The objective of this work was to develop a challenge model that effectively and repeatedly results in Salmonella-positive PLNs. A 10-lancet skin-allergy instrument was inoculated with Salmonella, and calves were inoculated intra- and/or transdermally by applying the device over various ventral regions of the skin. Salmonella was successfully and predictably recovered from region-specific PLNs up to 8 days postchallenge. Furthermore, serotypes inoculated within specific regions were only recovered from the PLNs draining those regions. This model provides a method to predictably infect PLNs with Salmonella. Further, this model makes it possible to determine the duration of infection and to evaluate candidate interventions that may shorten the duration of infection. C1 [Edrington, T. S.; Hill, J.; Genovese, K. J.; He, H.; Callaway, T. R.; Anderson, R. C.; Nisbet, D. J.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. [Loneragan, G. H.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. [Brichta-Harhay, D. M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Edrington, TS (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM tom.edrington@ars.usda.gov FU U.S. Department of Agriculture National Institutes of Food and Agriculture's National Integrated Food Safety Initiative [2011-51110-31081]; Beef Checkoff program FX This project was funded in part by the U.S. Department of Agriculture National Institutes of Food and Agriculture's National Integrated Food Safety Initiative award no 2011-51110-31081 and by the Beef Checkoff program. Dr. Loneragan has provided scientific advice to Pfizer Animal Health (the vaccine marketer) and Epitopix, LLC (the vaccine manufacturer) and has on occasion billed for this service. Dr. Loneragan has also received honoraria for service on advisory boards and presentations. No other financial conflicts of interest are reported. NR 10 TC 11 Z9 11 U1 0 U2 6 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2013 VL 76 IS 7 BP 1255 EP 1258 DI 10.4315/0362-028X.JFP-12-317 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FX UT WOS:000326081500018 PM 23834802 ER PT J AU Edrington, TS Loneragan, GH Hill, J Genovese, KJ Brichta-Harhay, DM Farrow, RL Krueger, NA Callaway, TR Anderson, RC Nisbet, DJ AF Edrington, T. S. Loneragan, G. H. Hill, J. Genovese, K. J. Brichta-Harhay, D. M. Farrow, R. L. Krueger, N. A. Callaway, T. R. Anderson, R. C. Nisbet, D. J. TI Development of Challenge Models To Evaluate the Efficacy of a Vaccine To Reduce Carriage of Salmonella in Peripheral Lymph Nodes of Cattle SO JOURNAL OF FOOD PROTECTION LA English DT Article ID IN-GROUND BEEF; DAIRY-CATTLE; PREVALENCE; ENTERICA; NEWPORT; COWS AB Because challenge models to infect peripheral lymph nodes (PLNs) with Salmonella have not been reported, we performed a series of experiments to develop and refine challenge models to evaluate an intervention applied at the animal level and to provide initial estimates of efficacy of an intervention (i.e., a vaccine) to aid in the design of future studies. In each of four experiments, steers (control or vaccinated) were inoculated with Salmonella strains Montevideo or Newport, and in experiment IV, Salmonella Senftenberg was also used. Calves were euthanized 14 to 42 days postinoculation, and PLNs were collected. In the first experiment, calves were challenged with similar to 10(10) Salmonella cells, and few treatment differences were observed 14 days postchallenge. However, by day 21, Salmonella Newport was recovered from fewer vaccinated calves than control calves (P < 0.05). In experiment II, calves were challenged with similar to 10(7) Salmonella cells and, after two necropsies (14 and 28 days postchallenge), only one lymph node was Salmonella positive; therefore, the study was terminated. In experiment III, calves were again challenged with similar to 10(10) Salmonella cells, and no significant effect of vaccine was observed in calves challenged with Montevideo or Newport strains. A transdermal route of challenge was explored in experiment IV, using a 10-lancet, allergy testing instrument. Sixteen steers were challenged with either Salmonella Newport or Salmonella Montevideo (Salmonella Newport right legs; Salmonella Montevideo left legs), and all steers were challenged on the lower abdomen with Salmonella Senftenberg. Transdermal inoculation resulted in predictably Salmonella-positive PLNs, and a modest vaccine effect was detected. Because it is well tolerated by the calves and results in predictable and regionally specific Salmonella recovery from PLNs, the transdermal route of challenge may he preferred by researchers wishing to evaluate the impact of interventions designed to reduce the carnage of Salmonella in PLNs. C1 [Edrington, T. S.; Hill, J.; Genovese, K. J.; Farrow, R. L.; Krueger, N. A.; Callaway, T. R.; Anderson, R. C.; Nisbet, D. J.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. [Loneragan, G. H.] Texas Tech Univ, Dept Anim & Food Sci, Lubbock, TX 79409 USA. [Brichta-Harhay, D. M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Edrington, TS (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM tom.edrington@ars.usda.gov FU U.S. Department of Agriculture National Institutes of Food and Agriculture's National Integrated Food Safety Initiative [2011-51110-31081]; Beef Checkoff program FX This project was funded by the U.S. Department of Agriculture National Institutes of Food and Agriculture's National Integrated Food Safety Initiative award no. 2011-51110-31081 and the Beef Checkoff program. Dr. Loneragan has provided scientific advice to Pfizer Animal Health (the vaccine marketer) and Epitopix, LLC (the vaccine manufacturer) and has on occasion billed for this service. Dr. Loneragan has also received honoraria for service on advisory boards and presentations. No other financial conflicts of interest are reported. NR 12 TC 11 Z9 11 U1 0 U2 9 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2013 VL 76 IS 7 BP 1259 EP 1263 DI 10.4315/0362-028X.JFP-12-319 PG 5 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FX UT WOS:000326081500019 PM 23834803 ER PT J AU Chen, CH Ravishankar, S Marchello, J Friedman, M AF Chen, Cynthia H. Ravishankar, Sadhana Marchello, John Friedman, Mendel TI Antimicrobial Activity of Plant Compounds against Salmonella Typhimurium DT104 in Ground Pork and the Influence of Heat and Storage on the Antimicrobial Activity SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157H7; ESSENTIAL OILS; CAMPYLOBACTER-JEJUNI; HETEROCYCLIC AMINES; SPOILAGE BACTERIA; SPICE EXTRACTS; BEEF PATTIES; ENTERICA; APPLE; CINNAMALDEHYDE AB Salmonella enterica is a predominant foodborne pathogen that causes diarrheal illness worldwide. A potential method of inhibiting pathogenic bacterial growth in meat is through the introduction of plant-derived antimicrobials. The objectives of this study were to investigate the influence of heat (70 degrees C for 5 min) and subsequent cold storage (4 degrees C. up to 7 days) on the effectiveness of oregano and cinnamon essential oils and powdered olive and apple extracts against Salmonella enterica serovar Typhimurium DT104 in ground pork and to evaluate the activity of the most effective antimicrobials (cinnamon oil and olive extract) at higher concentrations in heated ground pork. The surviving Salmonella populations in two groups (heated and unheated) of antimicrobial-treated pork were compared. Higher concentrations of the most effective compounds were then tested (cinnamon oil at 0.5 to 1.0% and olive extract at 3, 4, and 5%) against Salmonella Typhimurium in heated ground pork. Samples were stored at 4 degrees C and taken on days 0, 3, 5, and 7 or enumeration of survivors. The heating process did not affect the activity of antimicrobials. Significant 1.3- and 3-log reductions were observed with 1.0% cinnamon oil and 5% olive extract, respectively, on day 7. The minimum concentration required to achieve >1-log reduction in Salmonella population was 0.8% cinnamon oil or 4% olive extract. The results demonstrate the effectiveness of these antimicrobials against multidrug-resistant Salmonella Typhimurium in ground pork and their stability during heating and cold storage. The most active formulations have the potential to enhance the microbial safety of ground pork. C1 [Chen, Cynthia H.; Marchello, John] Univ Arizona, Dept Anim Sci, Tucson, AZ 85721 USA. [Ravishankar, Sadhana] Univ Arizona, Dept Vet Sci & Microbiol, Tucson, AZ 85721 USA. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Ravishankar, S (reprint author), Univ Arizona, Dept Anim Sci, Shantz 205,POB 210038, Tucson, AZ 85721 USA. EM sadhravi@email.arizona.edu OI Friedman, Mendel/0000-0003-2582-7517 NR 33 TC 6 Z9 6 U1 1 U2 12 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUL PY 2013 VL 76 IS 7 BP 1264 EP 1269 DI 10.4315/0362-028X.JFP-12-493 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FX UT WOS:000326081500020 PM 23834804 ER PT J AU D'Aiuto, PE Evens, TJ Albano, JP Patt, JM AF D'Aiuto, P. E. Evens, T. J. Albano, J. P. Patt, J. M. TI ALGAL TURF SCRUBBERS: PERIPHYTON PRODUCTION AND NUTRIENT RECOVERY ON A SOUTH FLORIDA CITRUS FARM SO PHYCOLOGIA LA English DT Meeting Abstract C1 [D'Aiuto, P. E.; Albano, J. P.; Patt, J. M.] USDA, Washington, DC USA. EM peter.daiuto@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 4 PU INT PHYCOLOGICAL SOC PI LAWRENCE PA NEW BUSINESS OFFICE, PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0031-8884 J9 PHYCOLOGIA JI Phycologia PD JUL PY 2013 VL 52 IS 4 SU S MA 56 BP 21 EP 21 PG 1 WC Plant Sciences; Marine & Freshwater Biology SC Plant Sciences; Marine & Freshwater Biology GA 238QV UT WOS:000325963600057 ER PT J AU Schleeweis, K Goward, SN Huang, CQ Masek, JG Moisen, G Kennedy, RE Thomas, NE AF Schleeweis, Karen Goward, Samuel N. Huang, Chengquan Masek, Jeffrey G. Moisen, Gretchen Kennedy, Robert E. Thomas, Nancy E. TI Regional dynamics of forest canopy change and underlying causal processes in the contiguous US SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE Forest disturbance; harvest; time-series; NAFD; Landsat ID EASTERN UNITED-STATES; TERRESTRIAL CARBON STORAGE; NORTH-AMERICAN FORESTS; NATURAL DISTURBANCES; LANDSCAPE PATTERNS; CLIMATE-CHANGE; CATASTROPHIC WIND; MAPPING WILDFIRE; FIRE MANAGEMENT; LANDSAT IMAGERY AB The history of forest change processes is written into forest age and distribution and affects earth systems at many scales. No one data set has been able to capture the full forest disturbance and land use record through time, so in this study, we combined multiple lines of evidence to examine trends, for six US regions, in forest area affected by harvest, fire, wind, insects, and forest conversion to urban/surburban use. We built an integrated geodatabase for the contiguous U.S. (CONUS) with data spanning the nation and decades, from remote sensing observations of forest canopy dynamics, geospatial data sets on disturbance and conversion, and statistical inventories, to evaluate relationships between canopy change observations and casual processes at multiple scales. Results show the variability of major change processes through regions across decades. Harvest affected more forest area than any other major change processes in the North East, North Central, Southeast, and South central regions. In the Pacific Coast and Intermountain West, more forest area was affected by harvest than forest fires. Canopy change rates at regional scales confounded the trends of individual forest change processes, showing the importance of landscape scale data. Local spikes in observed canopy change rates were attributed to wind and fire events, as well as volatile harvest regimes. This study improves the geographic model of forest change processes by updating regional trends for major disturbance and conversion processes and combining data on the dynamics of fire, wind, insects, harvest, and conversion into one integrated geodatabase for the CONUS. C1 [Schleeweis, Karen; Moisen, Gretchen] US Forest Serv, Rocky Mt Res Stn, USDA, Ogden, UT 84401 USA. [Schleeweis, Karen; Goward, Samuel N.; Huang, Chengquan; Thomas, Nancy E.] Univ Maryland, Dept Geog, College Pk, MD 20742 USA. [Masek, Jeffrey G.] NASA, Goddard Space Flight Ctr, Biospher Sci Branch, Greenbelt, MD 20771 USA. [Kennedy, Robert E.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Kennedy, Robert E.] Oregon State Univ, Dept Forest Sci, Corvallis, OR 97331 USA. [Thomas, Nancy E.] Stanford Univ, Dept Environm Earth Syst Sci, Stanford, CA 94305 USA. RP Schleeweis, K (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, Ogden, UT 84401 USA. EM kgschleeweis@fs.fed.us RI Masek, Jeffrey/D-7673-2012; OI Huang, Chengquan/0000-0003-0055-9798 FU NASA's Carbon Cycle Science Program [NNG05GE55G, NNX08AI26G, NNX11AJ78G] FX The study contributes to the North American Carbon Program (NACP), with grant support from NASA's Carbon Cycle Science Program (NNG05GE55G, NNX08AI26G, and NNX11AJ78G). The authors thank Brad Smith, the editor, and anonymous reviewers for their comments and feedback. NR 127 TC 2 Z9 2 U1 2 U2 21 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUL PY 2013 VL 118 IS 3 BP 1035 EP 1053 DI 10.1002/jgrg.20076 PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 233EV UT WOS:000325549900007 ER PT J AU Chen, GS Tian, HQ Huang, CQ Prior, SA Pan, SF AF Chen, Guangsheng Tian, Hanqin Huang, Chengquan Prior, Stephen A. Pan, Shufen TI Integrating a process-based ecosystem model with Landsat imagery to assess impacts of forest disturbance on terrestrial carbon dynamics: Case studies in Alabama and Mississippi SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE the southern United States; forest disturbance; Landsat imagery; carbon dynamics; Dynamic Land Ecosystem Model (DLEM) ID SOUTHERN UNITED-STATES; CHANGE TRACKER MODEL; ENVIRONMENTAL-CHANGES; TEMPERATE FORESTS; NORTH-AMERICA; STORAGE; CHINA; SEQUESTRATION; PINE; MANAGEMENT AB Forest ecosystems in the southern United States are dramatically altered by three major disturbances: timber harvesting, hurricane, and permanent land conversion. Understanding and quantifying effects of disturbance on forest carbon, nitrogen, and water cycles is critical for sustainable forest management in this region. In this study, we introduced a process-based ecosystem model for simulating forest disturbance impacts on ecosystem carbon, nitrogen, and water cycles. Based on forest mortality data classified from Landsat TM/ETM+images, this model was then applied to estimate changes in carbon storage using Mississippi and Alabama as a case study. Mean annual forest mortality rate for these states was 2.37%. Due to frequent disturbance, over 50% of the forest land in the study region was less than 30years old. Forest disturbance events caused a large carbon source (138.92 Tg C, 6.04 Tg C yr(-1); 1 Tg=10(12)g) for both states during 1984-2007, accounting for 2.89% (4.81% if disregard carbon storage changes in wood products) of the total forest carbon storage in this region. Large decreases and slow recovery of forest biomass were the main causes for carbon release. Forest disturbance could result in a carbon sink in few areas if wood product carbon was considered as a local carbon pool, indicating the importance of accounting for wood product carbon when assessing forest disturbance effects. The legacy effects of forest disturbance on ecosystem carbon storage could last over 50years. This study implies that understanding forest disturbance impacts on carbon dynamics is of critical importance for assessing regional carbon budgets. C1 [Chen, Guangsheng; Tian, Hanqin; Pan, Shufen] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Huang, Chengquan] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Prior, Stephen A.] USDA ARS Natl Soil Dynam Lab, Auburn, AL USA. RP Tian, HQ (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. EM tianhan@auburn.edu RI Tian, Hanqin/A-6484-2012; OI Tian, Hanqin/0000-0002-1806-4091; Huang, Chengquan/0000-0003-0055-9798 FU U.S. Department of Energy National Institute for Climate Change Research (NICCR) Program [DUKE-UN-07-SC-NICCR-1014]; NASA Interdisciplinary Science Program [NNX10AU06G]; NASA Terrestrial Ecology Program; Alabama Agricultural Experiment Station Research Program (AAES) FX This study has been supported by the U.S. Department of Energy National Institute for Climate Change Research (NICCR) Program (DUKE-UN-07-SC-NICCR-1014), NASA Interdisciplinary Science Program (NNX10AU06G), NASA Terrestrial Ecology Program, and Alabama Agricultural Experiment Station Research Program (AAES). We also thank three reviewers and Dennis Baldocchi for their constructive comments on the manuscript. NR 70 TC 8 Z9 8 U1 2 U2 18 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUL PY 2013 VL 118 IS 3 BP 1208 EP 1224 DI 10.1002/jgrg.20098 PG 17 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 233EV UT WOS:000325549900020 ER PT J AU Svanback, A Ulen, B Etana, A Bergstrom, L Kleinman, PJA Mattsson, L AF Svanback, Annika Ulen, Barbro Etana, Ararso Bergstrom, Lars Kleinman, Peter J. A. Mattsson, Lennart TI Influence of soil phosphorus and manure on phosphorus leaching in Swedish topsoils SO NUTRIENT CYCLING IN AGROECOSYSTEMS LA English DT Article DE Phosphorus leaching; Ammonium lactate-extractable soil P; Rainfall simulation; Long-term fertility experiments; Manure management ID PREFERENTIAL SOLUTE TRANSPORT; TERM FERTILITY EXPERIMENTS; CLAY SOIL; AGRICULTURAL SOILS; ORGANIC-MATTER; ANIMAL MANURE; SUBSURFACE; LOSSES; FORMS; FLOW AB In Sweden, subsurface transport of phosphorus (P) from agricultural soils represents the primary pathway of concern for surface water quality. However, there are mixed findings linking P in leachate with soil P and limited understanding of the interactive effects of applied P sources and soil test P on P leaching potential. Identifying soils that are susceptible to P leaching when manure is applied is critical to management strategies that reduce P loadings to water bodies. Intact soil columns (20 cm deep) from five long-term fertilization trials across Sweden were used in leaching experiments with simulated rainfall to explore the interactive effects of dairy cow (Bos taurus L.) manure application, soil test P and cropping system. Strong relationships were observed between ammonium lactate-extractable P in soil and dissolved reactive P (DRP) concentrations in leachate, although regression slopes varied across soils. For three soils, application of manure (equal to 21-30 kg P ha(-1)) to the soil columns significantly increased DRP leaching losses. The increase in DRP concentration was correlated to soil test P, but with wide variations between the three soils. For two soils leachate P concentrations after manure addition were independent of soil P status. Despite variable trends in P leaching across the different soils, P concentrations in leachate were always moderate from soils at fertilization rates equivalent to P removal with harvest. Results clearly stress the importance of long-term P balance to limit P leaching losses from Swedish agricultural soils. C1 [Svanback, Annika; Ulen, Barbro; Etana, Ararso; Bergstrom, Lars; Mattsson, Lennart] Swedish Univ Agr Sci SLU, Dept Soil & Environm, S-75007 Uppsala, Sweden. [Kleinman, Peter J. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Svanback, A (reprint author), Swedish Univ Agr Sci SLU, Dept Soil & Environm, POB 7014, S-75007 Uppsala, Sweden. EM Annika.Svanback@slu.se FU Swedish Farmers' Foundation for Agricultural Research FX This study was funded by the Swedish Farmers' Foundation for Agricultural Research. Ulf Olsson and Claudia von Bromssen (SLU) provided statistical advice. We are grateful to Ann Kristin Eriksson, Liselott Evasdotter, Pontus Johansson, Marcus Larsson, Stefan Ekberg, and Linnea Hedlof-Ekvall for their assistance in the field and laboratory. NR 59 TC 11 Z9 12 U1 3 U2 53 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-1314 EI 1573-0867 J9 NUTR CYCL AGROECOSYS JI Nutr. Cycl. Agroecosyst. PD JUL PY 2013 VL 96 IS 2-3 BP 133 EP 147 DI 10.1007/s10705-013-9582-9 PG 15 WC Soil Science SC Agriculture GA 237DN UT WOS:000325848600001 ER PT J AU Flannery, BG Spangler, RE Norcross, BL Lewis, CJ Wenburg, JK AF Flannery, Blair G. Spangler, Robert E. Norcross, Brenda L. Lewis, Cara J. Wenburg, John K. TI Microsatellite Analysis of Population Structure in Alaska Eulachon with Application to Mixed-Stock Analysis SO TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY LA English DT Article ID HIGH GENE FLOW; THALEICHTHYS-PACIFICUS; EFFECTIVE SIZE; LINKAGE DISEQUILIBRIUM; MARINE FISHES; ATLANTIC COD; SALMON; DIFFERENTIATION; IDENTIFICATION; CONSERVATION AB Estimation of genetic population structure, diversity, and effective population size (N-e ) is important for defining meaningful conservation units and assessing genetic health. Recent conservation concerns in Alaska have highlighted the need for research on Eulachon Thaleichthys pacificus, a species about which relatively little is known. Therefore, genetic variation was assayed at 14 microsatellite loci to investigate the genetic population structure of Alaska Eulachon. This analysis revealed a low degree of genetic divergence (G(ST) = 0.005) that is structured by broad-scale northern and southern geographic regions. Overall, there is a significant correlation between genetic and geographic distances, suggesting that gene flow is geographically restricted and follows an isolation-by-distance (IBD) model. However, closer analysis reveals an absence of IBD within regions and that gene flow is primarily restricted by the geographic distance between regions, a pattern that better approximates the hierarchical island model. Gene flow is likely restricted between regions by a biogeographical barrier (i.e., the Alexander Archipelago). Alaska Eulachon have high levels of genetic diversity and relatively large N-e estimates of 3,535 and 2,823 for the northern and southern regions, respectively, although a large variance in reproductive success is likely responsible for a low N-e /N ratio. The observed genetic divergence suggests that it would be advantageous to manage the two regions separately to maintain productivity and evolutionary potential for Eulachon. Mixed-stock analysis (MSA) of simulated and known-origin mixtures supports the feasibility of regional stock separation. The northern and southern regions had MSA accuracies that were near or greater than 90% when fish from a single region comprised the mixtures. Further population structure may be present within regions, but additional analyses of collections across sampling years are necessary to clarify the microevolutionary processes. Received June 7, 2012; accepted March 25, 2013 C1 [Flannery, Blair G.; Lewis, Cara J.; Wenburg, John K.] US Fish & Wildlife Serv, Conservat Genet Lab, Anchorage, AK 99503 USA. [Spangler, Robert E.] US Forest Serv, Anchorage, AK 99503 USA. [Norcross, Brenda L.] Univ Alaska Fairbanks, Sch Fisheries & Ocean Sci, Fairbanks, AK 99775 USA. RP Flannery, BG (reprint author), US Fish & Wildlife Serv, Conservat Genet Lab, 1011 East Tudor Rd,, Anchorage, AK 99503 USA. EM blair_flannery@fws.gov FU USFWS, Office of Subsistence Management, through the Fisheries Resource Monitoring Program [07-610] FX The U.S. Fish and Wildlife Service (USFWS) laboratory received partial funding support from the USFWS, Office of Subsistence Management, through the Fisheries Resource Monitoring Program, project 07-610. We thank the editors and two anonymous reviewers for comments that improved the manuscript. The findings and conclusions in this article are those of the authors and do not necessarily represent the views of the U.S. Fish and Wildlife Service, the University of Alaska-Fairbanks, or the U.S. Forest Service. NR 75 TC 1 Z9 1 U1 1 U2 14 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 0002-8487 EI 1548-8659 J9 T AM FISH SOC JI Trans. Am. Fish. Soc. PD JUL 1 PY 2013 VL 142 IS 4 BP 1036 EP 1048 DI 10.1080/00028487.2013.790841 PG 13 WC Fisheries SC Fisheries GA 220VB UT WOS:000324613900014 ER PT J AU Lokteff, RL Roper, BB Wheaton, JM AF Lokteff, Ryan L. Roper, Brett B. Wheaton, Joseph M. TI Do Beaver Dams Impede the Movement of Trout? SO TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY LA English DT Article ID BONNEVILLE CUTTHROAT TROUT; BROOK TROUT; SALVELINUS-FONTINALIS; UPSTREAM MOVEMENT; ATLANTIC SALMON; STREAM; HABITAT; RIVER; ECOSYSTEMS; LANDSCAPE AB Dams created by North American beavers Castor canadensis (hereafter, beavers) have numerous effects on stream habitat use by trout. Many of these changes to the stream are seen as positive, and many stream restoration projects seek either to reintroduce beavers or to mimic the habitat that they create. The extent to which beaver dams act as movement barriers to salmonids and whether successful dam passage differs among species are topics of frequent speculation and warrant further research. We investigated beaver dam passage by three trout species in two northern Utah streams. We captured 1,375 trout above and below 21 beaver dams and fitted them with PIT tags to establish whether fish passed the dams and to identify downstream and upstream passage; 187 individual trout were observed to make 481 passes of the 21 beaver dams. Native Bonneville Cutthroat Trout Oncorhynchus clarkii utah passed dams more frequently than nonnative Brown Trout Salmo trutta and nonnative Brook Trout Salvelinus fontinalis. We determined that spawn timing affected seasonal changes in dam passage for each species. Physical characteristics of dams, such as height and upstream location, affected the passage of each species. Movement behaviors of each trout species were also evaluated to help explain the observed patterns of dam passage. Our results suggest that beaver dams are not acting as movement barriers for Bonneville Cutthroat Trout or Brook Trout but may be impeding the movements of invasive Brown Trout. Received November 26, 2012; accepted April 15, 2013 C1 [Lokteff, Ryan L.; Roper, Brett B.] US Forest Serv, Fish & Aquat Ecol Unit, Logan, UT 84321 USA. [Lokteff, Ryan L.; Roper, Brett B.; Wheaton, Joseph M.] Utah State Univ, Dept Watershed Sci, Logan, UT 84322 USA. RP Lokteff, RL (reprint author), US Forest Serv, Fish & Aquat Ecol Unit, 860 North 1200 East, Logan, UT 84321 USA. EM rlokteff@gmail.com RI Wheaton, Joseph/F-1965-2010 OI Wheaton, Joseph/0000-0002-8361-8150 FU U.S. Forest Service; Intermountain Center for River Rehabilitation and Restoration FX We are indebted to Jared Randall, Jared Baker, Todd Wright, Sara Bangen, Alan Kasprak, Wes Gordon, and Ryan Leary for their extensive help with fish sampling and mobile antenna surveys. The clarity of the manuscript was improved by a helpful review from Florence Consolati. We thank Aggie Air, Nick Bouwes, Nadine Trahan, and Meagan Polino for providing aerial imagery with unmanned aerial vehicles. The U.S. Forest Service and the Intermountain Center for River Rehabilitation and Restoration provided funding for this research. NR 30 TC 6 Z9 6 U1 7 U2 74 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0002-8487 EI 1548-8659 J9 T AM FISH SOC JI Trans. Am. Fish. Soc. PD JUL 1 PY 2013 VL 142 IS 4 BP 1114 EP 1125 DI 10.1080/00028487.2013.797497 PG 12 WC Fisheries SC Fisheries GA 220VB UT WOS:000324613900021 ER PT J AU Pickett, STA Boone, CG McGrath, BP Cadenasso, ML Childers, DL Ogden, LA McHale, M Grove, JM AF Pickett, S. T. A. Boone, Christopher G. McGrath, Brian P. Cadenasso, M. L. Childers, Daniel L. Ogden, Laura A. McHale, Melissa Grove, J. Morgan TI Ecological science and transformation to the sustainable city SO CITIES LA English DT Article DE City; Ecology; Resilience; Sustainability; Urban ID URBAN; ECOSYSTEMS; RESILIENCE; BALTIMORE; FRAMEWORK; SYSTEMS; CITIES AB There is growing urgency to enhance the sustainability of existing and emerging cities. The science of ecology, especially as it interacts with disciplines in the social sciences and urban design, has contributions to make to the sustainable transformation of urban systems. Not all possible urban transformations may lead toward sustainability. Ecological science helps identify components of resilience that can favor transformations that are more sustainable. To summarize the dynamics and choices involved in sustainable,transformations, a "metacity" framework is presented, embracing ecological processes in cities as complementary to those involving society, power, and economy. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Pickett, S. T. A.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Boone, Christopher G.; Childers, Daniel L.] Arizona State Univ, Sch Sustainabil, Tempe, AZ 85287 USA. [McGrath, Brian P.] Parsons New Sch Design, New York, NY 10011 USA. [Cadenasso, M. L.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Ogden, Laura A.] Florida Int Univ, Sch Int & Publ Affairs, Dept Global & Sociocultural Studies, Miami, FL 33199 USA. [McHale, Melissa] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Grove, J. Morgan] US Forest Serv, Baltimore Field Stn, No Res Stn, Baltimore, MD 21228 USA. [Grove, J. Morgan] US Forest Serv, Baltimore Ecosyst Study, No Res Stn, Baltimore, MD 21228 USA. RP Pickett, STA (reprint author), Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. EM picketts@caryinstitute.org NR 82 TC 25 Z9 25 U1 5 U2 57 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-2751 J9 CITIES JI Cities PD JUL PY 2013 VL 32 SU 1 SI SI BP S10 EP S20 DI 10.1016/j.cities.2013.02.008 PG 11 WC Urban Studies SC Urban Studies GA 226RY UT WOS:000325054900003 ER PT J AU Averyt, K Meldrum, J Caldwell, P Sun, G McNulty, S Huber-Lee, A Madden, N AF Averyt, K. Meldrum, J. Caldwell, P. Sun, G. McNulty, S. Huber-Lee, A. Madden, N. TI Sectoral contributions to surface water stress in the coterminous United States SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE water resources; surface water; water stress ID CLIMATE-CHANGE; CHANGING CLIMATE; IMPACTS; AVAILABILITY; WITHDRAWALS; RESOURCES; DEMAND; US AB Here, we assess current stress in the freshwater system based on the best available data in order to understand possible risks and vulnerabilities to regional water resources and the sectors dependent on freshwater. We present watershed-scale measures of surface water supply stress for the coterminous United States (US) using the water supply stress index (WaSSI) model which considers regional trends in both water supply and demand. A snapshot of contemporary annual water demand is compared against different water supply regimes, including current average supplies, current extreme-year supplies, and projected future average surface water flows under a changing climate. In addition, we investigate the contributions of different water demand sectors to current water stress. On average, water supplies are stressed, meaning that demands for water outstrip natural supplies in over 9% of the 2103 watersheds examined. These watersheds rely on reservoir storage, conveyance systems, and groundwater to meet current water demands. Overall, agriculture is the major demand-side driver of water stress in the US, whereas municipal stress is isolated to southern California. Water stress introduced by cooling water demands for power plants is punctuated across the US, indicating that a single power plant has the potential to stress water supplies at the watershed scale. On the supply side, watersheds in the western US are particularly sensitive to low flow events and projected long-term shifts in flow driven by climate change. The WaSSI results imply that not only are water resources in the southwest in particular at risk, but that there are also potential vulnerabilities to specific sectors, even in the 'water-rich' southeast. C1 [Averyt, K.; Meldrum, J.] Univ Colorado, Cooperat Inst Res Environm Sci, Western Water Assessment, Boulder, CO 80309 USA. [Caldwell, P.; Sun, G.; McNulty, S.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC USA. [Huber-Lee, A.] Tufts Univ, Dept Civil & Environm Engn, Medford, MA 02155 USA. [Madden, N.] Union Concerned Scientists, Cambridge, MA USA. RP Averyt, K (reprint author), Univ Colorado, Cooperat Inst Res Environm Sci, Western Water Assessment, Boulder, CO 80309 USA. EM kristen.averyt@colorado.edu; james.meldrum@colorado.edu OI Meldrum, James/0000-0001-5250-3759 FU Union of Concerned Scientists; EW3 Scientific Advisory Committee; NOAA Western Water Assessment; Cooperative Institute for Research in Environmental Sciences at University of Colorado Boulder FX We greatly appreciate the support of the Union of Concerned Scientists, The Energy and Water in a Warming World initiative (EW3) contributors, and the EW3 Scientific Advisory Committee. Additional support was provided through the NOAA Western Water Assessment and the Cooperative Institute for Research in Environmental Sciences at University of Colorado Boulder. We are grateful to two anonymous reviewers whose comments and contributions greatly improved this letter. NR 37 TC 24 Z9 24 U1 2 U2 35 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD JUL-SEP PY 2013 VL 8 IS 3 AR 035046 DI 10.1088/1748-9326/8/3/035046 PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 229FL UT WOS:000325247100091 ER PT J AU Johnson, KD Harden, JW McGuire, AD Clark, M Yuan, FM Finley, AO AF Johnson, Kristofer D. Harden, Jennifer W. McGuire, A. David Clark, Mark Yuan, Fengming Finley, Andrew O. TI Permafrost and organic layer interactions over a climate gradient in a discontinuous permafrost zone SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE permafrost; discontinuous zone; organic soil; structural equation modeling; interior Alaska ID BLACK SPRUCE FOREST; INTERIOR ALASKA; BOREAL FORESTS; CARBON-CYCLE; FIRE; ACCUMULATION; MATTER; USA; DECOMPOSITION; VULNERABILITY AB Permafrost is tightly coupled to the organic soil layer, an interaction that mediates permafrost degradation in response to regional warming. We analyzed changes in permafrost occurrence and organic layer thickness (OLT) using more than 3000 soil pedons across a mean annual temperature (MAT) gradient. Cause and effect relationships between permafrost probability (PF), OLT, and other topographic factors were investigated using structural equation modeling in a multi-group analysis. Groups were defined by slope, soil texture type, and shallow (<28 cm) versus deep organic (>= 28 cm) layers. The probability of observing permafrost sharply increased by 0.32 for every 10-cm OLT increase in shallow OLT soils (OLTs) due to an insulation effect, but PF decreased in deep OLT soils (OLTd) by 0.06 for every 10-cm increase. Across the MAT gradient, PF in sandy soils varied little, but PF in loamy and silty soils decreased substantially from cooler to warmer temperatures. The change in OLT was more heterogeneous across soil texture types-in some there was no change while in others OLTs soils thinned and/or OLTd soils thickened at warmer locations. Furthermore, when soil organic carbon was estimated using a relationship with thickness, the average increase in carbon in OLTd soils was almost four times greater compared to the average decrease in carbon in OLTs soils across all soil types. If soils follow a trajectory of warming that mimics the spatial gradients found today, then heterogeneities of permafrost degradation and organic layer thinning and thickening should be considered in the regional carbon balance. C1 [Johnson, Kristofer D.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Harden, Jennifer W.] US Geol Survey, Menlo Pk, CA 94025 USA. [McGuire, A. David] Univ Alaska, US Geol Survey, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Clark, Mark] Nat Resources Conservat Serv, USDA, Palmer, AK 99645 USA. [Yuan, Fengming] Oak Ridge Natl Lab, Climate Change Sci Inst, Oak Ridge, TN 37831 USA. [Yuan, Fengming] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA. [Finley, Andrew O.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. [Finley, Andrew O.] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. RP Johnson, KD (reprint author), US Forest Serv, USDA, Newtown Sq, Newtown Sq, PA 19073 USA. EM kristoferdjohnson@fs.fed.us FU US Geological Survey; NSF [EF-1137309, EF-1253225]; NASA Carbon Monitoring System grants FX This assessment was sponsored by the US Geological Survey funded research on 'Assessing the Role of Deep Soil Organic Carbon in interior Alaska: Data, Models, and Spatial/Temporal Dynamics'. The soil pedon database provided by the USDA NRCS in Alaska was essential to study, as well as contributors to the Alaska Soil Carbon Database. AOF was supported by NSF EF-1137309 and EF-1253225, as well as NASA Carbon Monitoring System grants. NR 48 TC 16 Z9 16 U1 4 U2 42 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD JUL-SEP PY 2013 VL 8 IS 3 AR 035028 DI 10.1088/1748-9326/8/3/035028 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA 229FL UT WOS:000325247100073 ER PT J AU Dien, BS O'Bryan, PJ Hector, RE Iten, LB Mitchell, RB Qureshi, N Sarath, G Vogel, KP Cotta, MA AF Dien, Bruce S. O'Bryan, Patricia J. Hector, Ronald E. Iten, Loren B. Mitchell, Robert B. Qureshi, Nasib Sarath, Gautum Vogel, Kenneth P. Cotta, Michael A. TI Conversion of switchgrass to ethanol using dilute ammonium hydroxide pretreatment: influence of ecotype and harvest maturity SO ENVIRONMENTAL TECHNOLOGY LA English DT Article DE switchgrass; bioethanol; simultaneous saccharification and fermentation; pretreatment; bioenergy crop ID ENZYMATIC SACCHARIFICATION; BIOMASS YIELD; FERMENTATION; TECHNOLOGIES; ENZYMES; DIGESTIBILITY; RECALCITRANCE; INHIBITION; HYDROLYSIS; CELLULOSE AB Switchgrass (Panicum virgatum L.) is a perennial C4 grass that is being developed as a bioenergy crop because it has high production yields and suitable agronomic traits. Five switchgrass biomass samples from upland and lowland switchgrass ecotypes harvested at different stages or maturity were used in this study. Switchgrass samples contained 317.0-385.0gglucans/kg switchgrass dry basis (db) and 579.3-660.2g total structural carbohydrates/kg switchgrass, db. Carbohydrate contents were greater for the upland ecotype versus lowland ecotype and increased with harvest maturity. Pretreatment of switchgrass with dilute ammonium hydroxide (8%w/w ammonium loading) at 170 degrees C for 20min was determined to be effective for preparing switchgrass for enzymatic conversion to monosaccharides; glucose recoveries were 66.9-90.5% and xylose recoveries 60.1-84.2% of maximum and decreased with increased maturity at harvest. Subsequently, pretreated switchgrass samples were converted to ethanol by simultaneous saccharification and fermentation using engineered xylose-fermenting Saccharomyces cerevisiae strain YRH400. Ethanol yields were 176.2-202.0l/Mg of switchgrass (db) and followed a similar trend as observed for enzymatic sugar yields. C1 [Dien, Bruce S.; O'Bryan, Patricia J.; Hector, Ronald E.; Iten, Loren B.; Qureshi, Nasib; Cotta, Michael A.] ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Mitchell, Robert B.; Sarath, Gautum; Vogel, Kenneth P.] Univ Nebraska, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Dien, BS (reprint author), ARS, Bioenergy Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM bruce.dien@ars.usda.gov OI Dien, Bruce/0000-0003-3863-6664 FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011 68005-30411] FX This work was supported in part by Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [grant number 2011 68005-30411]. NR 46 TC 7 Z9 7 U1 1 U2 15 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0959-3330 J9 ENVIRON TECHNOL JI Environ. Technol. PD JUL 1 PY 2013 VL 34 IS 13-14 SI SI BP 1837 EP 1848 DI 10.1080/09593330.2013.833640 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA 231CF UT WOS:000325389900014 PM 24350437 ER PT J AU Ren, JS Yu, YC Gao, FY Zeng, LH Lu, XJ Wu, XT Yan, WG Ren, GJ AF Ren, Juansheng Yu, Yuchao Gao, Fangyuan Zeng, Lihua Lu, Xianjun Wu, Xianting Yan, Wengui Ren, Guangjun TI Application of resistance gene analog markers to analyses of genetic structure and diversity in rice SO GENOME LA English DT Article DE RGA marker; genetic structure; diversity; rice; SSR marker ID PLANT-DISEASE RESISTANCE; ORYZA-SATIVA L.; WHEAT STRIPE RUST; NBS-LRR CLASS; CULTIVATED RICE; LOCAL ADAPTATION; PATHOGEN INTERACTIONS; POLYMORPHISM MARKERS; ARABIDOPSIS-THALIANA; POPULATION-STRUCTURE AB Plant disease resistance gene analog (RGA) markers were designed according to the conserved sequence of known RGAs and used to map resistance genes. We used genome-wide RGA markers for genetic analyses of structure and diversity in a global rice germplasm collection. Of the 472 RGA markers, 138 were polymorphic and these were applied to 178 entries selected from the USDA rice core collection. Results from the RGA markers were similar between two methods, UPGMA and STRUCTURE. Additionally, the results from RGA markers in our study were agreeable with those previously reported from SSR markers, including cluster of ancestral classification, genetic diversity estimates, genetic relatedness, and cluster of geographic origins. These results suggest that RGA markers are applicable for analyses of genetic structure and diversity in rice. However, unlike SSR markers, the RGA markers failed to differentiate temperate japonica, tropical japonica, and aromatic subgroups. The restricted way for developing RGA markers from the cDNA sequence might limit the polymorphism of RGA markers in the genome, thus limiting the discriminatory power in comparison with SSR markers. Genetic differentiation obtained using RGA markers may be useful for defining genetic diversity of a suite of random R genes in plants, as many studies show a differentiation of resistance to a wide array of pathogens. They could also help to characterize the genetic structure and geographic distribution in crops, including rice, wheat, barley, and banana. C1 [Ren, Juansheng; Yu, Yuchao; Gao, Fangyuan; Lu, Xianjun; Wu, Xianting; Ren, Guangjun] Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China. [Yu, Yuchao; Zeng, Lihua] Sichuan Normal Univ, Chengdu 610066, Peoples R China. [Yan, Wengui] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Yan, WG (reprint author), ARS, USDA, Dale Bumpers Natl Rice Res Ctr, 2890 Hwy 130 East, Stuttgart, AR 72160 USA. EM Wengui.Yan@ars.usda.gov; guangjun61@sina.com FU National Science Foundation of China; Sichuan Youth Science & Technology Innovative Research Team Foundation; National High-Tech R&D Program of China FX We thank E. McWhirter, Y. Jia, and A. McClung (Dale Bumpers National Rice Research Center, United States Department of Agriculture-Agricultural Research Service, Stuttgart, AR, USA) for their critical review. This research project was supported by grants from the National Science Foundation of China, Sichuan Youth Science & Technology Innovative Research Team Foundation, and National High-Tech R&D Program of China. NR 99 TC 3 Z9 3 U1 1 U2 9 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD JUL PY 2013 VL 56 IS 7 BP 377 EP 387 DI 10.1139/gen-2012-0142 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 233BZ UT WOS:000325541200002 PM 24099390 ER PT J AU Liu, FJ Hunsaker, C Bales, RC AF Liu, Fengjing Hunsaker, Carolyn Bales, Roger C. TI Controls of streamflow generation in small catchments across the snow-rain transition in the Southern Sierra Nevada, California SO HYDROLOGICAL PROCESSES LA English DT Article DE streamflow generation; snow-rain transition; endmember mixing analysis; southern Sierra Nevada ID PONDEROSA PINE HILLSLOPE; WESTERN NORTH-AMERICA; UNITED-STATES; SUBSURFACE FLOW; CLIMATE-CHANGE; LATERAL FLOW; NEW-MEXICO; PATHWAYS; RUNOFF; FOREST AB Processes controlling streamflow generation were determined using geochemical tracers for water years 2004-2007 at eight headwater catchments at the Kings River Experimental Watersheds in southern Sierra Nevada. Four catchments are snow-dominated, and four receive a mix of rain and snow. Results of diagnostic tools of mixing models indicate that Ca2+, Mg2+, K+ and Cl- behaved conservatively in the streamflow at all catchments, reflecting mixing of three endmembers. Using endmember mixing analysis, the endmembers were determined to be snowmelt runoff (including rain on snow), subsurface flow and fall storm runoff. In seven of the eight catchments, streamflow was dominated by subsurface flow, with an average relative contribution (% of streamflow discharge) greater than 60%. Snowmelt runoff contributed less than 40%, and fall storm runoff less than 7% on average. Streamflow peaked 2-4weeks earlier at mixed rain-snow than snow-dominated catchments, but relative endmember contributions were not significantly different between the two groups of catchments. Both soil water in the unsaturated zone and regional groundwater were not significant contributors to streamflow. The contributions of snowmelt runoff and subsurface flow, when expressed as discharge, were linearly correlated with streamflow discharge (R-2 of 0.85-0.99). These results suggest that subsurface flow is generated from the soil-bedrock interface through preferential pathways and is not very sensitive to snow-rain proportions. Thus, a declining of the snow-rain ratio under a warming climate should not systematically affect the processes controlling the streamflow generation at these catchments. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Liu, Fengjing; Bales, Roger C.] Univ Calif, Sierra Nevada Res Inst, Merced, CA USA. [Liu, Fengjing] Lincoln Univ Missouri, Dept Agr & Environm Sci, Jefferson City, MO USA. [Liu, Fengjing] Lincoln Univ Missouri, Cooperat Res Programs, Jefferson City, MO USA. [Hunsaker, Carolyn] US Forest Serv, Pacific Southwest Res Stn, USDA, Fresno, CA USA. RP Liu, FJ (reprint author), Lincoln Univ, Dept Agr & Environm Sci, 904 Chestnut St, Jefferson City, MO 65101 USA. EM liuf@lincolnu.edu OI Hunsaker, Carolyn/0000-0002-5662-2402 FU Forest Service, USDA; California's State Water Resources Control Board; National Science Foundation (NSF), through the Southern Sierra Critical Zone Observatory [EAR-0725097]; National Science Foundation (NSF) [BES-0610112]; Evans-Allen project [225140]; Capacity Building Grant [2011-02451]; National Institute of Food and Agriculture (NIFA), USDA FX The authors appreciate the sample collection by T. Whitaker and other staff at Pacific Southwest Research Station, Forest Service, US Department of Agriculture (USDA), Fresno, California. This research was supported by the Forest Service, USDA; California's State Water Resources Control Board through Proposition 50 (the Water Security, Clean Drinking Water, Coastal, and Beach Protection Act of 2002); and the National Science Foundation (NSF), through the Southern Sierra Critical Zone Observatory (EAR-0725097) and grant BES-0610112 to the University of California, Merced. This research was also supported in part by an Evans-Allen project (225140) and a Capacity Building Grant (2011-02451), funded through the National Institute of Food and Agriculture (NIFA), USDA. NR 38 TC 11 Z9 11 U1 2 U2 40 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 J9 HYDROL PROCESS JI Hydrol. Process. PD JUL 1 PY 2013 VL 27 IS 14 BP 1959 EP 1972 DI 10.1002/hyp.9304 PG 14 WC Water Resources SC Water Resources GA 228VG UT WOS:000325219100002 ER PT J AU Zhang, T Wilson, GV AF Zhang, T. Wilson, G. V. TI Spatial distribution of pipe collapses in Goodwin Creek Watershed, Mississippi SO HYDROLOGICAL PROCESSES LA English DT Article DE pipe flow; collapse; erosion; topography; drainage area; slope ID EROSION PATTERNS; GULLY EROSION; CATCHMENTS; INITIATION; TILLAGE; RUNOFF AB The internal erosion of soil pipes can induce pipe collapses that affect soil erosion processes and landform evolution. The objective of this study was to determine the spatial distribution of pipe collapses in agricultural fields of Goodwin Creek watershed. Ground survey was carried out to detect pipe collapses, and the location, size and surface elevation was measured with differential GPS. A total of 143 of the 145 pipe collapses were found in cropland, and the density was approximately 0.58 collapses per hectare. The spatial distribution of pipe collapses was not uniform as pipe collapses were concentrated in the flat alluvial plains where the land use was dominated by cropland. One of the four parcels had 90% of the pipe collapses with a density of 7.7 collapses per hectare. The mean depth, area and volume of these pipe collapses were 0.12 m, 0.34 m(2) and 0.02 m(3), respectively, and all these properties exhibited a skewed distribution. The drainage area-slope gradient equation, which has been widely used for erosion phenomenon prediction, did not represent pipe collapses in this study as the coefficient of determination was <0.01. This is clear evidence that subsurface flow is not represented by surface topographic characteristics. The pipe collapses were found to intercept runoff, thereby reducing the slope length factor by 6% and the drainage area by 7%. Both of these factors can reduce the sheet and rill erosion; however, the increased subsurface flow could enhance ephemeral gully erosion. Published 2012. This article is a U.S. Government work and is in the public domain in the USA. C1 [Zhang, T.] Beijing Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Wilson, G. V.] ARS, Watershed Phys Proc Res Unit, Natl Sedimentat Lab, USDA, Oxford, MS 38655 USA. RP Wilson, GV (reprint author), ARS, Natl Sedimentat Lab, USDA, 598 McElroy Dr, Oxford, MS 38655 USA. EM glenn.wilson@ars.usda.gov FU US Department of Agriculture; Chinese Academy of Sciences, China Scholarship Council FX This work was supported by the US Department of Agriculture and the Chinese Academy of Sciences, China Scholarship Council funds. The authors express their appreciation to Mr Alan Hudspeth and Mr Allen Gregory for their outstanding technical support. NR 29 TC 6 Z9 6 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 J9 HYDROL PROCESS JI Hydrol. Process. PD JUL 1 PY 2013 VL 27 IS 14 BP 2032 EP 2040 DI 10.1002/hyp.9357 PG 9 WC Water Resources SC Water Resources GA 228VG UT WOS:000325219100007 ER PT J AU Ledoux, T Hilmers, A Watson, K Baranowski, T O'Connor, TM AF Ledoux, Tracey Hilmers, Angela Watson, Kathy Baranowski, Tom O'Connor, Teresia M. TI Development and Feasibility of an Objective Measure of Patient-Centered Communication Fidelity in a Pediatric Obesity Intervention SO JOURNAL OF NUTRITION EDUCATION AND BEHAVIOR LA English DT Article DE patient centered; health education; child obesity; primary care; health behavior ID PREVENTION; RECOMMENDATIONS; CONSEQUENCES; METAANALYSIS; OVERWEIGHT; CHILDHOOD; CHILDREN AB Objective: To develop a measure of person-centered communication (PCC) and demonstrate feasibility for use in primary care child obesity interventions. Methods: Helping Healthy Activity and Nutrition Directions was a primary care intervention for families of overweight or obese 5- to 8-year-old children. The PCC Coding System (PCCCS) was based on theory and a validated motivational interviewing instrument. The PCCCS provided global scores, and total, positive, and negative PCC utterance frequencies. Three trained coders tested reliability of the PCCCS on audio recordings of sessions with 30 families. Potential uses of the PCCCS were demonstrated. Results: The PCCCS demonstrated good inter-rater reliability for utterance frequencies but not for global scores. Conclusions and Implications: The PCCCS is a reliable and feasible measure of PCC utterances. More research is needed to improve inter-rater reliability of the PCC global scale. The PCCCS may be used in the future to test fidelity of PCC interventions. C1 [Ledoux, Tracey] Univ Houston, Dept Hlth & Human Performance, Houston, TX 77204 USA. [Hilmers, Angela; Watson, Kathy; Baranowski, Tom; O'Connor, Teresia M.] Baylor Coll Med, USDA, Agr Res Serv, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Ledoux, T (reprint author), Univ Houston, 3855 Holman St,104 Garrison Gym, Houston, TX 77204 USA. EM TALedoux@uh.edu OI Hilmers, Angela/0000-0001-7640-0308; Baranowski, Tom/0000-0002-0653-2222 FU United States Department of Agriculture (USDA); Gillson Longenbaugh Foundation (Baylor College of Medicine); USDA/Agricultural Research Service [58-6250-6001] FX This research was funded by the United States Department of Agriculture (USDA) and the Gillson Longenbaugh Foundation (Baylor College of Medicine). In addition, this work is from scientists at the USDA/Agricultural Research Service Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine and Texas Children's Hospital, Houston, Texas. This project has been funded in part by federal funds from the USDA/Agricultural Research Service under Cooperative Agreement 58-6250-6001. The contents of this publication do not necessarily reflect the views or policies of the USDA or mention of trade names, commercial products, or organizations imply endorsement by the United States Government. NR 24 TC 1 Z9 1 U1 0 U2 6 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1499-4046 J9 J NUTR EDUC BEHAV JI J. Nutr. Educ. Behav. PD JUL-AUG PY 2013 VL 45 IS 4 BP 349 EP 354 DI 10.1016/j.jneb.2012.10.006 PG 6 WC Education, Scientific Disciplines; Nutrition & Dietetics SC Education & Educational Research; Nutrition & Dietetics GA 222SE UT WOS:000324750600012 PM 23395302 ER PT J AU Trappe, JM Castellano, MA Hailling, RE Osmundson, TW Binder, M Fechner, N Malajczuk, N AF Trappe, James M. Castellano, Michael A. Hailling, Roy E. Osmundson, Todd W. Binder, Manfred Fechner, Nigel Malajczuk, Nicholas TI Australasian sequestrate fungi 18: Solioccasus polychromus gen. & sp nov., a richly colored, tropical to subtropical, hypogeous fungus SO MYCOLOGIA LA English DT Article DE Basidiomycota; Boletales; Boletineae; Bothia; DNA; ectomycorrhizae; EF1-alpha.; ITS; LSU; rhizomorphs; tef1 ID MULTIPLE SEQUENCE ALIGNMENT; DIVERSIFICATION; SYSTEMATICS; BOLETALES; MAFFT; DNA AB Solioccasus polychromus gen. & sp. nov., the most brightly colored hypogeous fungus known, is described from Papua New Guinea and tropical northern Australia south into subtropical forests along the Queensland coast and coastal mountains to near Brisbane. Phylogenetic analysis of molecular data places it as a sister genus to Bothia in the Boletineae, a clade of predominantly ectomycorrhizal boletes. Ectomycorrhizal trees, such as members of the Myrtaceae (Eucalyptus, Corymbia, Lophostemon, Melaleuca spp.) and Allocasuarina littoralis, were present usually in mixture or in some cases dominant, so we infer some or all of them to be among the ectomycorrhizal hosts of S. polychromus. C1 [Trappe, James M.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Castellano, Michael A.] US Forest Serv, USDA, No Res Stn, Corvallis, OR 97331 USA. [Hailling, Roy E.] New York Bot Garden, Bronx, NY 10458 USA. [Osmundson, Todd W.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Forest Pathol & Mycol Lab, Berkeley, CA 94720 USA. [Binder, Manfred] Inst Royal Netherlands Acad Arts & Sci KNAW, CBS Fungal Biodivers Ctr, NL-3584 CT Utrecht, Netherlands. [Fechner, Nigel] Queensland Herbarium, Brisbane, Qld 4066, Australia. [Malajczuk, Nicholas] Treetec Consulting Pty Ltd, Bunbury, WA 6231, Australia. RP Trappe, JM (reprint author), Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. EM trappej@onid.orst.edu RI Binder, Manfred/C-8571-2013 FU Australian Centre for International Agricultural Research [8736, 9116]; Australian Biological Resources Study; U.S. National Science Foundation [DEB 9007186]; National Science Foundation [DEB 1020421]; National Geographic Society Committee for Research and Exploration [8457-08] FX We thank the Australian Centre for International Agricultural Research for financially supporting projects 8736 and 9116 for N. Malajczuk and Australian Biological Resources Study for its grant to T. Lebel, J. Trappe and R Hailing (Revisibn of Australian truffle-like genera in the order Boletales, Basidiomycota). U.S. National Science Foundation supported travel by Trappe and Castellano through Grant DEB 9007186. The U.S. Forest Service, Pacific Northwest Research Station, contributed laboratory and office facilities in support of Trappe's participation in the research. Roy Hailing acknowledges support from National Science Foundation grant DEB 1020421 and National Geographic Society Committee for Research and Exploration grant 8457-08. The Queensland Parks and Wildlife Service provided accommodation and orientation on Fraser Island Sushma Mandava and Matthew Sewell engineered the extraction and amplification of DNA from the Fraser Island specimen in the laboratory of the Lewis B. and Domthy Cullman Program for Molecular Systematics at the New York Botanical Garden. We appreciate the excellent service by the staffs of herbaria in providing access to and accessioning of these collections: the Queensland Herbarium, Brisbane (BRI), Papua New Guinea Forest Research Institute Herbarium, Lae (LAE), National Herbarium of Victoria, South Yarra (MEL), New York Botanical Garden, Bronx (NY), Oregon State University Herbarium, Corvallis (OSC), Western Australian Herbarium, Perth (PERTH) and Papua New Guinea Division of Primary Industry Herbarium (PNG). Sam Malish of the Papua New Guinea Forest Research Institute and Brian Gunn of the Australian Commonwealth Institute for Scientific and Industrial Research Division of Forestry and Forest Products efficiently organized and guided the collecting trips in Papua New Guinea, as did Paul Reddell of CSIRO Forestry and Forest Products for tropical Australia. Roy Young, Katawe Baku, M. Bisam, Neale Bougher, Victoria Gordon, K. Querengasser and G. Tubeg, additional participants in the field work, merit our deeply felt thanks for putting up with tropical temperatures and humidity and often living and working in primitive conditions. NR 19 TC 10 Z9 10 U1 0 U2 12 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2013 VL 105 IS 4 BP 888 EP 895 DI 10.3852/12-046 PG 8 WC Mycology SC Mycology GA 197KG UT WOS:000322849500009 PM 23709482 ER PT J AU Hubka, V Kolarik, M Kubatova, A Peterson, SW AF Hubka, Vit Kolarik, Miroslav Kubatova, Alena Peterson, Stephen W. TI Taxonomic revision of Eurotium and transfer of species to Aspergillus SO MYCOLOGIA LA English DT Article DE food spoilage; low water activity; multilocus sequence typing; scanning electron microscopy; stored grain; xerophilic species ID DNA-SEQUENCES; COLOR MUTANTS; IDENTIFICATION; FUNGI; CHEVALIERI; TOXICITY; RECOGNITION; OTOMYCOSIS; FUMIGATUS; INFERENCE AB Aspergillus section Aspergillus contains economically important, xerophilic fungi that are widely distributed in nature and the human environment and are known for their ability to grow on substrates with low water activity. The taxa were revised based on sequence data from four loci, PCR fingerprinting, micro- and macromorphology, and physiology. The number of taxa was reduced to 17 species, all of which can be distinguished with sequence data from either the caM or RPB2 locus. The original description of A. proliferans was supplemented by a description of its teleomorph. This species seems to be relatively common and often has been confused with A. glaucus. In addition, green sporulating isolates of A. niveoglaucus isolated from food and several other substrates are indistinguishable in phenotype from A. glaucus. A dichotomous key based on ascospore size and ornamentation and the ability to grow at specific combinations of temperature and water activity is provided for identification of species. In response to recent changes in the botanical code, we transferred the Eurotium species to Aspergillus and selected one name for each species. C1 [Hubka, Vit] AS CR, Inst Microbiol, Lab Fungal Genet & Metab, Vvi, Prague 14220 4, Czech Republic. [Hubka, Vit; Kolarik, Miroslav; Kubatova, Alena] Charles Univ Prague, Fac Sci, Dept Bot, Prague 12801 2, Czech Republic. [Peterson, Stephen W.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Hubka, V (reprint author), AS CR, Inst Microbiol, Lab Fungal Genet & Metab, Vvi, Videnska 1083, Prague 14220 4, Czech Republic. EM hubka@biomed.cas.cz RI Kolarik, Miroslav/A-1680-2008; Hubka, Vit/L-4124-2014; Kubatova, Alena/D-7918-2016 OI Kolarik, Miroslav/0000-0003-4016-0335; Hubka, Vit/0000-0003-4583-6496; Kubatova, Alena/0000-0002-2837-1087 FU Grant Agency of the Czech Republic [P506/12/1064]; Ministry of Education, Youth and Sports of the Czech Republic; Charles University [267208/13]; [GAUK 607812] FX This work was supported by the Grant Agency of the Czech Republic P506/12/1064, institutional resources of Ministry of Education, Youth and Sports of the Czech Republic for the support of science and research and Charles University Research Project No. 267208/13. Molecular genetic analyses were supported by project GAUK 607812. Prof Jiri Vana and Prof Karol Marhold are gratefully acknowledged for consultation on nomenclatural changes. We thank Blanka Sramkova, Dr Pavlina Lyskova, Dr Magdalena Skorepova, Dr Alena Novakova., Dr Jiri Rehulka, Dr Monika Laichmanova and Dr Nada Mallatova for supplying isolates. We also thank Dr Miroslav Hylis for help with scanning electron microscopy, Dr Milada Chudickova for isolation of DNA and Dagmar Kozakova for lyophilization of the cultures. We are grateful to Dr Bruce W. Horn for careful editing. NR 90 TC 28 Z9 30 U1 2 U2 29 PU ALLEN PRESS INC PI LAWRENCE PA 810 E 10TH ST, LAWRENCE, KS 66044 USA SN 0027-5514 J9 MYCOLOGIA JI Mycologia PD JUL-AUG PY 2013 VL 105 IS 4 BP 912 EP 937 DI 10.3852/12-151 PG 26 WC Mycology SC Mycology GA 197KG UT WOS:000322849500011 PM 23396159 ER PT J AU Browne, GT AF Browne, Greg T. TI Outlook Specialty crops and methyl bromide alternatives: taking stock after 7 years SO CALIFORNIA AGRICULTURE LA English DT Editorial Material C1 Univ Calif Davis, Dept Plant Pathol, USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Browne, GT (reprint author), Univ Calif Davis, Dept Plant Pathol, USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 2 U2 3 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 123 EP 124 DI 10.3733/ca.v067n03p123 PG 2 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800003 ER PT J AU Browne, GT Lampinen, BD Holtz, BA Doll, DA Upadhyaya, SK Schmidt, LS Bhat, RG Udompetaikul, V Coates, RW Hanson, BD Klonsky, KM Gao, SD Wang, D Gillis, M Gerik, JS Johnson, RS AF Browne, Greg T. Lampinen, Bruce D. Holtz, Brent A. Doll, David A. Upadhyaya, Shrinivasa K. Schmidt, Leigh S. Bhat, Ravindra G. Udompetaikul, Vasu Coates, Robert W. Hanson, Bradley D. Klonsky, Karen M. Gao, Suduan Wang, Dong Gillis, Matt Gerik, James S. Johnson, R. Scott TI Managing the almond and stone fruit replant disease complex with less soil fumigant SO CALIFORNIA AGRICULTURE LA English DT Article ID MANAGEMENT AB As much as one-third of California's almond and stone fruit acreage is infested with potentially debilitating plant parasitic nematodes, and even more of the land is impacted by Prunus replant disease (PRD), a poorly understood soilborne disease complex that suppresses early growth and cumulative yield in replanted almond and peach orchards. Preplant soil fumigation has controlled these key replant problems, but the traditional fumigant of choice, methyl bromide, has been phased out, and other soil fumigants are increasingly regulated and expensive. We tested fumigant and nonfumigant alternatives to methyl bromide in multiple-year replant trials. Costs and benefits were evaluated for alternative fumigants applied by shanks in conventional strip and full-coverage treatments and applied by shanks or drip in novel spot treatments that targeted tree planting sites. Short-term sudan-grass rotation and prudent rootstock selection were examined as nonfumigant approaches to managing PRD. Trial results indicated that integrations of the treatments may acceptably control PRD with relatively little soil fumigant. C1 [Browne, Greg T.; Schmidt, Leigh S.] Univ Calif Davis, USDA ARS, Davis, CA 95616 USA. [Browne, Greg T.; Schmidt, Leigh S.; Bhat, Ravindra G.; Hanson, Bradley D.] Univ Calif Davis, Dept Plant Pathol, Davis, CA USA. [Lampinen, Bruce D.] Univ Calif Davis, Davis, CA USA. [Upadhyaya, Shrinivasa K.; Udompetaikul, Vasu; Coates, Robert W.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA USA. [Udompetaikul, Vasu] King Mongkuts Inst Technol, Dept Agr Engn, Ladkrabang, Thailand. [Klonsky, Karen M.] Univ Calif Davis, Dept Agr & Resource Econ, Davis, CA USA. [Gao, Suduan; Wang, Dong; Gerik, James S.] USDA ARS, Parlier, CA USA. [Johnson, R. Scott] Univ Calif Kearney Agr Res & Extens Ctr, Parlier, CA USA. RP Browne, GT (reprint author), Univ Calif Davis, USDA ARS, Davis, CA 95616 USA. FU Pacific Area-Wide Pest Management Program, USDA-ARS; Almond Board of California; UC Agricultural Experiment Station; TriCal Inc.; Paramount Farming, Agriland Farming, Bauer Farming; Harry Berberian and Sons; Donald Ewy, Mike Stanghellini, Burchell Nursery Inc.; Duarte Nursery Inc. FX The authors gratefully acknowledge support and assistance from the Pacific Area-Wide Pest Management Program, USDA-ARS, the Almond Board of California, the UC Agricultural Experiment Station, TriCal Inc., Paramount Farming, Agriland Farming, Bauer Farming, Harry Berberian and Sons, Donald Ewy, Mike Stanghellini, Burchell Nursery Inc., and Duarte Nursery Inc. NR 20 TC 4 Z9 4 U1 5 U2 41 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 128 EP 138 DI 10.3733/ca.v067n03p128 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800005 ER PT J AU Fennimore, SA Serohijos, R Samtani, JB Ajwa, HA Subbarao, KV Martin, FN Daugovish, O Legard, D Browne, GT Muramoto, J Shennan, C Klonsky, K AF Fennimore, Steven A. Serohijos, Raquel Samtani, Jayesh B. Ajwa, Husein A. Subbarao, Krishna V. Martin, Frank N. Daugovish, Oleg Legard, Dan Browne, Greg T. Muramoto, Joji Shennan, Carol Klonsky, Karen TI TIF film, substrates and nonfumigant soil disinfestation maintain fruit yields SO CALIFORNIA AGRICULTURE LA English DT Article ID METHYL-BROMIDE FUMIGATION; SEEDED SCOTS PINE; MICROBIAL COMMUNITIES; STEAM STERILIZATION; IMPERMEABLE FILM; WEED-CONTROL; STRAWBERRY; CHLOROPICRIN; FUMIGANTS; CULTURE AB A 5-year project to facilitate the adoption of strawberry production systems that do not use methyl bromide initially focused on fumigant alternatives and resulted in increased use of barrier films that reduce fumigant emissions. The focus shifted in year 3 to evaluating and demonstrating nonfumigant alternatives: soilless production, biofumigation, anaerobic soil disinfestation (ASD) and disinfestation with steam. In the 2010-2011 strawberry production season, fruit yields on substrates were comparable to fruit yields using conventional methods. Anaerobic soil disinfestation and steam disinfestation also resulted in fruit yields that were comparable to those produced using conventionally fumigated soils. Additional work is in progress to evaluate their efficacy in larger-scale production systems in different strawberry production districts in California. C1 [Fennimore, Steven A.; Serohijos, Raquel; Ajwa, Husein A.] Univ Calif Davis, Dept Plant Sci, Salinas, CA USA. [Samtani, Jayesh B.] Virginia Polytech Inst & State Univ, Virginia Beach, VA USA. [Subbarao, Krishna V.] Univ Calif Davis, Dept Plant Pathol, Salinas, CA USA. [Martin, Frank N.] USDA ARS, Salinas, CA USA. [Legard, Dan] Calif Strawberry Commiss, Watsonville, CA USA. [Browne, Greg T.] USDA ARS, Davis, CA USA. [Muramoto, Joji; Shennan, Carol] Univ Calif Santa Cruz, Dept Environm Studies, Santa Cruz, CA 95064 USA. [Klonsky, Karen] Univ Calif Davis, Davis, CA USA. RP Fennimore, SA (reprint author), Univ Calif Davis, Dept Plant Sci, Salinas, CA USA. RI Shennan, Carol/I-1694-2013; OI Shennan, Carol/0000-0001-6401-5007; Subbarao, Krishna/0000-0002-2075-1835 FU USDA-ARS Pacific Area-Wide Pest Management Program for Methyl Bromide Alternatives FX We are grateful for the financial support of the USDA-ARS Pacific Area-Wide Pest Management Program for Methyl Bromide Alternatives for support of this project. We thank TriCal, Mustard Product Technologies and Raven Industries for material and technical support. NR 41 TC 8 Z9 8 U1 2 U2 35 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 EI 2160-8091 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 139 EP 146 DI 10.3733/ca.v067n03p139 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800006 ER PT J AU Ajwa, H Stanghellini, MS Gao, S Sullivan, DA Khan, A Ntow, W Qin, RJ AF Ajwa, Husein Stanghellini, Michael S. Gao, Suduan Sullivan, David A. Khan, Afiqur Ntow, William Qin, Ruijun TI Fumigant emission reductions with TIF warrant regulatory changes SO CALIFORNIA AGRICULTURE LA English DT Article ID 1,3-DICHLOROPROPENE; CHLOROPICRIN; SOIL AB With methyl bromide's phase-out, most growers have turned to alternative fumigants, particularly 1,3-dichloropropene (1,3-D) and chloropicrin. These alternatives are tightly regulated because they are classified as toxic air contaminants and volatile organic compounds; the latter combine with other substances to produce ground-level ozone (smog). Two ambient air monitoring studies were conducted to evaluate the potential of totally impermeable film (TIF) to reduce emissions from shank applications of chloropicrin and 1,3-D. In 2009, a study demonstrated that TIF reduced chloropicrin and 1,3-D peak emissions by 45% and 38%, respectively, but TIF did not reduce total emissions when it was cut after 6 days. In 2011, increasing the tarp period from 5 to 10 days decreased chloropicrin and 1,3-D peak emissions by 88% and 78%, and their total emissions by 64% and 43%, respectively. Concurrent dynamic flux chamber results corroborated the ambient air monitoring data. These studies provide regulatory agencies with mitigation measures that should allow continued fumigant use at efficacious application rates. C1 [Ajwa, Husein; Khan, Afiqur; Qin, Ruijun] Univ Calif Davis, Davis, CA 95616 USA. [Stanghellini, Michael S.] TriCal Inc, Hollister, CA USA. [Gao, Suduan] USDA ARS, Washington, DC 20250 USA. [Sullivan, David A.] Sullivan Environm Consulting Inc, Alexandria, VA USA. RP Ajwa, H (reprint author), Univ Calif Davis, Davis, CA 95616 USA. NR 13 TC 3 Z9 3 U1 1 U2 10 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 147 EP 152 DI 10.3733/ca.v067n03p147 PG 6 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800007 ER PT J AU Weiland, JE Littke, WR Haase, DL AF Weiland, Jerry E. Littke, Will R. Haase, Diane L. TI Forest nurseries face critical choices with the loss of methyl bromide fumigation SO CALIFORNIA AGRICULTURE LA English DT Review ID PACIFIC-NORTHWEST; SOIL FUMIGATION; MANAGEMENT; PATHOGENS; CHLOROPICRIN; DEGRADATION; SEEDLINGS; BAREROOT AB Forest nurseries in the western United States have relied for decades on methyl bromide to control soilborne pests. Numerous studies have investigated alternative fumigants, alternative application methods and nonfumigant approaches for their ability to reduce soilborne pest populations and produce quality, disease-free seedlings. We review the recent studies and identify where research is needed to assist the industry's transition away from methyl bromide. For the immediate, foreseeable future, an integrated approach combining nonfumigant and fumigant methods will provide the best strategy. Nevertheless, the industry may need to transition completely to container production if fumigant regulations become more restrictive. C1 [Weiland, Jerry E.] USDA ARS, Hort Crops Res Unit, Washington, DC 20250 USA. [Haase, Diane L.] USDA, Forest Serv, Washington, DC USA. RP Weiland, JE (reprint author), USDA ARS, Hort Crops Res Unit, Washington, DC 20250 USA. NR 45 TC 6 Z9 6 U1 0 U2 5 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 153 EP 161 DI 10.3733/ca.v067n03p153 PG 9 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800008 ER PT J AU Suddick, EC Ngugi, MK Paustian, K Six, J AF Suddick, Emma C. Ngugi, Moffatt K. Paustian, Keith Six, Johan TI Monitoring soil carbon will prepare growers for a carbon trading system SO CALIFORNIA AGRICULTURE LA English DT Article ID ORGANIC-CARBON; CLIMATE-CHANGE; CALIFORNIA; EMISSIONS; STABILIZATION AB California growers could reap financial benefits from the low-carbon economy and cap-and-trade system envisioned by the state's AB 32 law, which seeks to lower greenhouse gas emissions statewide. Growers could gain carbon credits by reducing greenhouse gas emissions and sequestering carbon through reduced tillage and increased biomass residue incorporation. First, however, baseline stocks of soil carbon need to be assessed for various cropping systems and management practices. We designed and set up a pilot soil carbon and land-use monitoring network at several perennial cropping systems in Northern California. We compared soil carbon content in two vineyards and two orchards (walnut and almond), looking at conventional and conservation management practices, as well as in native grassland and oak woodland. We then calculated baseline estimates of the total carbon in almond, wine grape and walnut acreages statewide. The organic walnut orchard had the highest total soil carbon, and no-till vineyards had 27% more carbon in the surface soil than tilled vineyards. We estimated wine grape vineyards are storing significantly more soil carbon per acre than almond and walnut orchards. The data can be used to provide accurate information about soil carbon stocks in perennial cropping systems for a future carbon trading system. C1 [Suddick, Emma C.; Ngugi, Moffatt K.; Six, Johan] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Suddick, Emma C.] Woods Hole Res Ctr, Woods Hole, MA USA. [Ngugi, Moffatt K.] USAID, USDA FAS, Washington, DC USA. [Paustian, Keith] Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA. [Six, Johan] Swiss Fed Inst Technol, World Food Syst Ctr, Zurich, Switzerland. RP Suddick, EC (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RI Paustian, Keith/L-7593-2016 FU United States Department of Agriculture (USDA) Natural Resources Conservation Service (NRCS) FX Funding was provided by the United States Department of Agriculture (USDA) Natural Resources Conservation Service (NRCS). We wish to acknowledge Robert Rousseau for his excellent help both in the laboratory and in the field and Cherry Lai for all her help with microbalancing. NR 20 TC 0 Z9 1 U1 2 U2 38 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 162 EP 171 DI 10.3733/ca.v067n03p162 PG 10 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800009 ER PT J AU Hanson, BD Gao, SD Gerik, J Qin, RJ Cabrera, JA Jhala, AJ Abit, MJM Cox, D Correiar, B Wang, D Browne, GT AF Hanson, Bradley D. Gao, Suduan Gerik, James Qin, Ruijun Cabrera, J. Alfonso Jhala, Amit J. Abit, M. Joy M. Cox, David Correiar, Brian Wang, Dong Browne, Gregory T. TI Preplant 1,3-D treatments test well for perennial crop nurseries, but challenges remain SO CALIFORNIA AGRICULTURE LA English DT Article ID METHYL-BROMIDE; FIELD NURSERIES; ALTERNATIVES; FUMIGANTS; FILM AB Preplant fumigation with methyl bromide commonly is used in open-field perennial crop nurseries in California for control of plant-parasitic nematodes, pathogens and weeds. Because this fumigant is being phased out, alternatives are needed to ensure the productivity of the perennial crop nursery industry as well as the ornamental, orchard and vineyard production systems that depend on clean planting stock. As part of the USDA Area-Wide Pest Management Program for Integrated Methyl Bromide Alternatives, several perennial crop nursery projects were conducted in California from 2007 to 2011 to test and demonstrate registered alternative fumigants and application techniques that maximize performance and minimize environmental impacts. The project was designed to evaluate shank application and soil surface sealing methods intended to reduce aboveground emission and improve soil performance of 1,3-dichloropropene, a leading methyl bromide alternative for nurseries. In these garden rose and tree nursery experiments, 1,3-dichloropropene treatments performed well regardless of application techniques. In this article, we highlight recent research and discuss the significance and remaining challenges for adoption of methyl bromide alternatives in this unique nursery stock production system. C1 [Hanson, Bradley D.; Qin, Ruijun; Jhala, Amit J.; Abit, M. Joy M.] Univ Calif Davis, Davis, CA 95616 USA. [Gao, Suduan; Gerik, James; Wang, Dong; Browne, Gregory T.] USDA ARS, Washington, DC 20250 USA. [Cabrera, J. Alfonso] Univ Calif Riverside, Riverside, CA 92521 USA. RP Hanson, BD (reprint author), Univ Calif Davis, Davis, CA 95616 USA. OI Jhala, Amit/0000-0001-8599-4996 FU USDA-ARS Pacific Area-Wide Pest Management Program for Integrated Methyl Bromide Alternatives; Fruit Tree; Nut Tree; Grapevine Improvement Advisory Board FX This project was primarily supported by the USDA-ARS Pacific Area-Wide Pest Management Program for Integrated Methyl Bromide Alternatives. Funding from the Fruit Tree, Nut Tree, and Grapevine Improvement Advisory Board and in-kind support from Weeks Rose Nursery, Dave Wilson Nursery, TriCal Inc., and Dow AgroSciences for these projects is acknowledged. The technical support of Stella Zambrzuski, Nancy Goodell, Jim Gartung, Patty Mungur, Robert Shenk, Aileen Hendratna and other USDA-ARS Water Management Research Unit staff is also appreciated. NR 16 TC 5 Z9 5 U1 0 U2 7 PU UNIV CALIFORNIA, OAKLAND, DIVISION AGRICULTURE & NATURAL RESOURCES PI RICHMOND PA 1301 S 46 ST, RICHMOND, CA 94804 USA SN 0008-0845 J9 CALIF AGR JI Calif. Agric. PD JUL-SEP PY 2013 VL 67 IS 3 SI SI BP 181 EP 189 DI 10.3733/ca.E.v067n03p181 PG 9 WC Agriculture, Multidisciplinary SC Agriculture GA 216TQ UT WOS:000324307800012 ER PT J AU Sun, JH Lin, LZ Chen, P AF Sun, Jianghao Lin, Longze Chen, Pei TI Recent Applications for HPLC-MS Analysis of Anthocyanins in Food Materials SO CURRENT ANALYTICAL CHEMISTRY LA English DT Article DE Anthocyanins; separation; detection; HPLC; UHPLC; HRMS; HPLC-MS ID PERFORMANCE LIQUID-CHROMATOGRAPHY; TANDEM-MASS-SPECTROMETRY; PHOTODIODE-ARRAY DETECTION; FLESHED SWEET-POTATO; BLOOD ORANGE JUICE; RED WINE; HUMAN PLASMA; ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; L. BERRIES AB Anthocyanins are an important group of polyphenols that have health promoting properties. Analytical techniques for profiling anthocyanins have been widely reported in the last decade for in vitro and in vivo studies. A number of important technological advances in high-performance liquid chromatography (HPLC) and the introduction of the ultra high-performance liquid chromatography (UHPLC) have achieved significant gains in both the speed and the separation of anthocyanins in the past decade. Advancements in mass spectrometry, such as high-resolution and sequential collision mass spectrometry (HRMS and MSn) allow fast structural elucidation of food anthocyanins that play a very important role in food anthocyanin research. A significant increase in the number of studies has been reported for tandem MS, HRMS, and MSn based approaches for food analysis. This article provides an updated review of the application of liquid chromatography (HPLC and UHPLC) in combination with mass spectrometry (MS) for the study of food anthocyanins in recent years. C1 [Sun, Jianghao; Lin, Longze; Chen, Pei] ARS, USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD USA. RP Chen, P (reprint author), ARS, USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD USA. EM pei.chen@ars.usda.gov FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements of the National Institutes of Health FX This research is supported by the Agricultural Research Service of the U.S. Department of Agriculture and an Interagency Agreement with the Office of Dietary Supplements of the National Institutes of Health. NR 195 TC 5 Z9 5 U1 4 U2 36 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 1573-4110 J9 CURR ANAL CHEM JI Curr. Anal. Chem. PD JUL PY 2013 VL 9 IS 3 BP 397 EP 416 PG 20 WC Chemistry, Analytical SC Chemistry GA 219FW UT WOS:000324492600008 ER PT J AU Vellas, B Pahor, M Manini, T Rooks, D Guralnik, JM Morley, J Studenski, S Evans, W Asbrand, C Fariello, R Pereira, S Rolland, Y Van Kan, GA Cesari, M Chumlea, WC Fielding, R AF Vellas, B. Pahor, M. Manini, T. Rooks, D. Guralnik, J. M. Morley, J. Studenski, S. Evans, W. Asbrand, C. Fariello, R. Pereira, S. Rolland, Y. Van Kan, G. Abellan Cesari, M. Chumlea, Wm. C. Fielding, R. TI Designing pharmaceutical trials for sarcopenia in frail older adults: EU/US task force recommendations SO JOURNAL OF NUTRITION HEALTH & AGING LA English DT Article DE Sarcopenia; clinical trial; skeletal muscle; frailty; intervention ID RANDOMIZED CONTROLLED-TRIAL; LIFE-STYLE INTERVENTIONS; SKELETAL-MUSCLE; PHYSICAL PERFORMANCE; CLINICAL-TRIALS; PROTEIN SUPPLEMENTATION; MEANINGFUL CHANGE; HIP FRACTURE; WEIGHT-LOSS; BLOOD-FLOW AB An international task force of academic and industry leaders in sarcopenia research met on December 5, 2012 in Orlando, Florida to develop guidelines for designing and executing randomized clinical trials of sarcopenia treatments. The Task Force reviewed results from previous trials in related disease areas to extract lessons relevant to future sarcopenia trials, including practical issues regarding the design and conduct of trials in elderly populations, the definition of appropriate target populations, and the selection of screening tools, outcome measures, and biomarkers. They discussed regulatory issues, the challenges posed by trials of different types of interventions, and the need for standardization and harmonization. The Task Force concluded with recommendations for advancing the field toward better clinical trials. C1 [Vellas, B.; Rolland, Y.; Cesari, M.] Fac Med Toulouse, INSERM, UMR1027, F-31073 Toulouse, France. [Vellas, B.; Rolland, Y.; Cesari, M.] Univ Toulouse 3, F-31062 Toulouse, France. [Vellas, B.; Rolland, Y.; Van Kan, G. Abellan; Cesari, M.] Toulouse Univ Hosp, Gerontopole, Toulouse, France. [Pahor, M.; Manini, T.] Univ Florida, Gainesville, FL USA. [Rooks, D.] Novartis Inst Biomed Res, Cambridge, MA USA. [Guralnik, J. M.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. [Morley, J.] St Louis Univ, Sch Med, St Louis, MO USA. [Studenski, S.] Univ Pittsburgh, Pittsburgh, PA USA. [Evans, W.] GlaxoSmithKline, Res Triangle Pk, NC USA. [Asbrand, C.] Sanofi Aventis Deutschland GmbH, Frankfurt, Germany. [Fariello, R.] Neurotune AG, CH-8952 Schlieren, Switzerland. [Pereira, S.] Abbott Nutr R&D, Columbus, OH USA. [Van Kan, G. Abellan; Chumlea, Wm. C.] Wright State Univ, Boonshoft Sch Med, Lifespan Hlth Res Ctr, Dayton, OH 45435 USA. [Fielding, R.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Vellas, B (reprint author), Univ Toulouse 3, F-31062 Toulouse, France. RI Cesari, Matteo/A-4649-2008 OI Cesari, Matteo/0000-0002-0348-3664 FU NIA NIH HHS [P30 AG028740]; NICHD NIH HHS [R01 HD012252] NR 51 TC 12 Z9 12 U1 4 U2 13 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 1279-7707 J9 J NUTR HEALTH AGING JI J. Nutr. Health Aging PD JUL PY 2013 VL 17 IS 7 BP 612 EP 618 DI 10.1007/s12603-013-0362-7 PG 7 WC Geriatrics & Gerontology; Nutrition & Dietetics SC Geriatrics & Gerontology; Nutrition & Dietetics GA 226GG UT WOS:000325022200005 PM 23933872 ER PT J AU Spooner, D Rojas, P Bonierbale, M Mueller, LA Srivastav, M Senalik, D Simon, P AF Spooner, David Rojas, Percy Bonierbale, Meredith Mueller, Lukas A. Srivastav, Manish Senalik, Douglas Simon, Philipp TI Molecular Phylogeny of Daucus (Apiaceae) SO SYSTEMATIC BOTANY LA English DT Article DE Carrot; taxonomy; Umbelliferae ID SUBFAMILY APIOIDEAE; GENETIC-VARIABILITY; INTRON SEQUENCES; CAROTA COMPLEX; CARROT; EVOLUTIONARY; CAUCALIDEAE; REVEALS; DIVERSITY; MARKERS AB We studied the phylogeny of 22 accessions of Daunts and seven accessions in related genera, with DNA sequences from eight nuclear orthologs and one plastid (psbA/trnH) region. Maximum parsimony and maximum likelihood analyses of the concatenated data matrix of 7,212 aligned nucleotides provided excellent bootstrap support for many clades. Concordant with prior molecular results Pseudorlaya pumila was firmly imbedded within Daucus, as was Margottia gummifera, a new finding. All accessions of D. capillifolius, D. carota, and D. sahariensis formed a 2n = 18 clade with all other species within the Daucus clade with chromosome numbers of = 20, 22, and 44 (D. glochidiatus). Sister to the D. carota clade was a clade containing Margottia gummifera and Pseudorlaya pumila, sister to these species was D. crinitus, sister to all the above was D. muricatus, and sister to all of the above was a clade containing the remaining Daucus species. Bayesian analyses of individual regions analyzed separately and averaged over multiple trees with *BEAST software (a coalescent approach), however, provided a phylogeny at variance with the concatenated approach, most notably in firmly imbedding Turgenia latifolia within Daucus. C1 [Spooner, David; Rojas, Percy; Senalik, Douglas; Simon, Philipp] Univ Wisconsin, USDA, Agr Res Serv, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Bonierbale, Meredith] Int Potato Ctr, Lima 12, Peru. [Mueller, Lukas A.] Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Srivastav, Manish] Indian Agr Res Inst, Div Fruits & Hort Technol, New Delhi 110012, India. RP Spooner, D (reprint author), Univ Wisconsin, USDA, Agr Res Serv, Vegetable Crops Res Unit, 1575 Linden Dr, Madison, WI 53706 USA. EM david.spooner@ars.usda.gov FU USDA National Research Initiative [2008-35300-18669]; NSF DEB [0949121] FX We thank Cecile Ane, Brian Just, and Patricia Lu-Irving for advice on *BEAST analyses; Holly Ruess for technical assistance; and David Brenner and Kathleen Reitsma at the North Central Regional Plant Introduction Station in Ames, Iowa for providing germplasm. This work was supported by USDA National Research Initiative grant number 2008-35300-18669 to DS, MB, and LM; and by NSF DEB (grant number 0949121) to DS, MB, and LM. NR 46 TC 12 Z9 13 U1 2 U2 19 PU AMER SOC PLANT TAXONOMISTS PI LARAMIE PA UNIV WYOMING, DEPT BOTANY 3165, 1000 E UNIVERSITY AVE, LARAMIE, WY 82071 USA SN 0363-6445 J9 SYST BOT JI Syst. Bot. PD JUL-SEP PY 2013 VL 38 IS 3 BP 850 EP 857 DI 10.1600/036364413X670449 PG 8 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA 223YY UT WOS:000324849600025 ER PT J AU Armijo, CB Foulk, JA Whitelock, DP Hughs, SE Holt, GA Gillum, MN AF Armijo, C. B. Foulk, J. A. Whitelock, D. P. Hughs, S. E. Holt, G. A. Gillum, M. N. TI FIBER AND YARN PROPERTIES FROM HIGH-SPEED ROLLER GINNING OF UPLAND COTTON SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Cottonseed quality; Fiber quality; Ring spinning; Roller ginning; Upland cotton; Yarn quality AB Selective breeding has improved upland cotton fiber properties. New high-speed roller ginning technology has advanced to the point that processing capacities have approached that of saw ginning. Producers seeking a better price for their upland cotton are interested in fiber property and textile mill performance data comparing cultivars processed by both saw and roller ginning. Three diverse cultivars, one of them stripper-harvested, were processed by saw ginning, conventional roller ginning, and high-speed roller ginning, with different levels of lint cleaning appropriate to each treatment. Samples were submitted for HVI and AFIS fiber property analysis. Ring-spun yarn from each treatment was tested. Results showed that the roller gin (conventional and high speed) produced fiber that was longer more uniform, had less short fiber, and fewer neps than the saw gin stand. Turnout, color grade, and leaf were not different among gin stand type. With respect to yarn properties, the conventional/high-speed roller gin had fewer thick places, but was higher in vegetable and foreign dark matter (contaminants), seed coats, and neps. The composition of neps changed as fiber was processed into yarn. The conventional/high-speed roller gin had fewer raw stock and card mat neps than the saw gin stand, but more neps in finished yarn. Differences jamong cultivar were prevalent throughout the study. In addition to differences in length, strength, and immature fiber content, the cultivar that was stripper harvested had double the trash content at harvest. There were differences in most fiber properties, but not yarn properties, among lint cleaner type. Roller gin lint cleaning was less aggressive than saw-type lint cleaning and had longer fiber, better uniformity, and fewer neps. Saw-type lint cleaning had better color and leaf grades, and less lint trash. There was no appreciable difference between the two types of roller gin lint cleaning used, but one saw-type lint cleaner as opposed to two was less damaging to the fiber Although roller ginned upland cotton is a niche market, textile mills value the higher quality obtained from roller ginning. Newer upland cultivars may make roller ginning a viable option in parts of the Unites States where roller ginning has not been available previously. C1 [Armijo, C. B.] ARS, USDA, Southern Plains Area, Southwestern Cotton Ginning Res Lab SWCGRL, Mesilla Pk, NM 88047 USA. [Foulk, J. A.] Clemson Univ, Clemson, SC USA. [Whitelock, D. P.; Hughs, S. E.; Holt, G. A.] ARS, USDA, Southern Plains Area, Cotton Prod & Proc Unit, Lubbock, TX USA. RP Armijo, CB (reprint author), ARS, USDA, Southern Plains Area, Southwestern Cotton Ginning Res Lab, POB 578, Mesilla Pk, NM 88047 USA. EM cararmij@nmsu.edu NR 20 TC 1 Z9 1 U1 1 U2 3 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2013 VL 29 IS 4 BP 461 EP 471 PG 11 WC Agricultural Engineering SC Agriculture GA 214RW UT WOS:000324154500003 ER PT J AU Martin, DE AF Martin, D. E. TI FLOW VARIABILITY OF AN AERIAL VARIABLE-RATE NOZZLE AT CONSTANT PRESSURES SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Variable rate application; Nozzles; Aerial spraying; Agricultural aviation AB Variable-rate ground application systems have been in use for the past 15 years, but due to high application speeds, flow requirements, and aerodynamic considerations, variable-rate aerial nozzles have not been available until now. In 2006, Spray Target, Inc. (Rosemount, Minn.) released the VeriRateTM variable-rate aerial nozzle, the first commercially-available retrofit aerial nozzle of its kind. Conventional, fixed-orifice, hydraulic aerial nozzles are physically and practically limited to a doubling of flowrate. This new variable-orifice aerial nozzle promised a 10X rate change. Using a standard aircraft spray boom, this study quantified flowrate and flowrate variability from a set of 48 VeriRate nozzles at spray pressures ranging from 207-483 kPa (30-70 psi). Results indicated a 3.4 fold increase in flowrate change (2.35 to 8.10 L min(-1)) that could be achieved with this nozzle, but shy of the desired 10X rate change. Additionally, measured flowrate from the nozzles was dependent on the pressure-up sequence. Excess flow variability (CV>25%) between nozzles was found when spray pressure was gradually applied to the nozzles. Initially "spiking" the spray pressure to 483 kPa (70 psz) removed virtually all excess flow variability, except at 207 kPa (30 psi). Aerial applicators will be able to use the results from this study to make informed decisions when selecting variable-rate aerial nozzles. C1 [Martin, D. E.] ARS, USDA, College Stn, TX USA. RP Martin, DE (reprint author), 2771 F&B Rd, College Stn, TX 77845 USA. EM Daniel.Martin@ars.usda.gov NR 10 TC 0 Z9 0 U1 0 U2 3 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2013 VL 29 IS 4 BP 483 EP 488 PG 6 WC Agricultural Engineering SC Agriculture GA 214RW UT WOS:000324154500005 ER PT J AU O'Shaughnessy, SA Urrego, YF Evett, SR Colaizzi, PD Howell, TA AF O'Shaughnessy, S. A. Urrego, Y. F. Evett, S. R. Colaizzi, P. D. Howell, T. A. TI ASSESSING APPLICATION UNIFORMITY OF A VARIABLE RATE IRRIGATION SYSTEM IN A WINDY LOCATION SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Application uniformity; Low-quarter distribution; Catch cans; Coefficient of uniformity; Pulse on/off; Variable rate irrigation ID DROP SIZE DISTRIBUTIONS; SPRINKLER SYSTEM; WATER; LOSSES; DRIFT; EVAPORATION; COLLECTORS; SAMPLES AB Variable rate irrigation (VRI) systems are commercially available and can easily be retrofitted onto moving sprinkler systems. However, there are few reports on the application peiformance of such equipment. In this study, application uniformity of two center pivots equipped with a commercial VRI system were tested using fixed-plate sprinklers and a range of irrigation rates (100%, 80%, 70%, 50%, and 30%). Catch cans were arranged in transect, arcwise, and grid patterns to test the accuracy of application depth, and the uniformity of application in the direction of pivot travel and to investigate changes in uniformity along the pivot lateral between irrigation zones. The mean Heermann and Hein coefficient of uniformity (CUHH) and the mean lower-quarter distribution uniformity (DUlp) in the direction of pivot travel and along the pivot lateral were approximately 88% and 80%, respectively. Application uniformity was impacted at the border of adjacent irrigation zones along the pivot lateral when zones were applying different irrigation depths. While wind speed and direction did not appreciably decrease uniformity of application, it did impact absolute catch measurements. The mean evaporation and drift loss for all trials was 9.3%, and ranged from I% to 19%. Applying variable rate irrigation signcantly impacted uniformity of application for a distance 3 m wide between irrigation zones of different irrigation depths in spans 1-3 of the three-span, and a distance 6 to 9 m wide between irrigation zones in spans 5 and 6 of the six-span center pivot. Overall, the uniformity of application in the direction of pivot travel and within each irrigation zone was similar to values reported for other VRI sprinkler systems. The root mean square error between the prescribed and actual applied depth was <3.0 mm for catch collected in all spans along the pivot lateral and in the direction of pivot travel. This indicates that this commercial VRI is well suited for site-specific irrigation management. However; uniformity of application could be affected by the width of the irrigation management zones as well as sprinkler design and environmental factors. C1 [O'Shaughnessy, S. A.; Urrego, Y. F.; Evett, S. R.] CSIC, EEAD, Zaragoza 50059, Spain. [Colaizzi, P. D.; Howell, T. A.] ARS, USDA, Bushland, TX USA. RP O'Shaughnessy, SA (reprint author), PO Drawer 10, Bushland, TX 79012 USA. EM susan.oshaughnessy@ars.usda.gov FU USDA-ARS Ogallala Aquifer Program; USDA-Agricultural Research Service; Kansas State University; Texas AgriLife Research; Texas AgriLife Extension Service; Texas Tech University; West Texas AM University FX We gratefully acknowledge support from the USDA-ARS Ogallala Aquifer Program, a consortium between USDA-Agricultural Research Service, Kansas State University, Texas AgriLife Research, Texas AgriLife Extension Service, Texas Tech University, and West Texas A&M University. Work reported in this article was accomplished as part of a Cooperative Research and Development Agreement between USDA-ARS and Valmont Industries, Inc., Valley, Nebraska (Agreement No.: 58-3K95-0-1455-M). The dedicated efforts of Mr. Luke Britten, Agricultural Science Research Technician, and Mr. Brice B. Ruthardt, Biological Science Technician, are gratefully acknowledged. NR 43 TC 9 Z9 10 U1 2 U2 7 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 EI 1943-7838 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2013 VL 29 IS 4 BP 497 EP 510 PG 14 WC Agricultural Engineering SC Agriculture GA 214RW UT WOS:000324154500007 ER PT J AU Quintanar, AI Mahmood, R Lovanh, N Rawley, JM Becerra-Acosta, E Loughrin, JH AF Quintanar, A. I. Mahmood, R. Lovanh, N. Rawley, J. M. Becerra-Acosta, E. Loughrin, J. H. TI ESTIMATING GREENHOUSE GAS EMISSIONS FROM A WASTE LAGOON SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Greenhouse gas (GHG); CO2; CH4; N2O; Energy flux; Livestock waste lagoon ID NET-RADIATION FLUX; BOWEN-RATIO; MALODOROUS COMPOUNDS; SOIL-MOISTURE; HEAT-FLUX; EVAPORATION; EVAPOTRANSPIRATION; TEMPERATURE; SURFACE; LOSSES AB A cost-effective approach was used to investigate the relationship between emission of the greenhouse gases (GHG), namely, CO2, CH4, and N2O and energy fluxes from a swine waste lagoon. Energy fluxes were calculated using the Penman method. The energy fluxes showed a diurnal pattern as expected of such fluxes. We found that air temperature and latent energy, lagoon surface temperature and solar radiation, as well as air temperature and wind speed can be used to predict for CO2, CH4, and N2O emissions, respectively. Comparison of observed and predicted emissions provided r(2) values of 0.49, 0.61, and 0.69 for CH4, N2O, and CO2, respectively. This research shows that long-term studies of GHG emissions and meteorological conditions are necessary to better understand the factors controlling the emissions of GHG in order to devise best management practices (BMP) for their control. C1 [Mahmood, R.] Western Kentucky Univ, Dept Geog & Geol, Bowling Green, KY 42101 USA. [Lovanh, N.; Loughrin, J. H.] USDA ARS, Anim Waste Management Res Unit, Bowling Green, KY USA. [Rawley, J. M.; Becerra-Acosta, E.] Western Kentucky Univ, Bowling Green, KY 42101 USA. [Rawley, J. M.] Univ S Carolina, Columbia, SC 29208 USA. RP Mahmood, R (reprint author), Western Kentucky Univ, Dept Geog & Geol, 1906 Coll Hts Blvd, Bowling Green, KY 42101 USA. EM rezaul.mahmood@wku.edu FU USDA [58-6445-6-068]; NSF REU [1004655] FX The authors thank two reviewers and the editor for their valuable comments and suggestions which helped to improve this manuscript. This work is funded by the USDA grant #58-6445-6-068. It also benefitted from a NSF REU grant #1004655. The authors thank Joe St. Claire, and Marty Haley for construction and maintenance of field equipment. NR 48 TC 1 Z9 1 U1 2 U2 12 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2013 VL 29 IS 4 BP 511 EP 519 PG 9 WC Agricultural Engineering SC Agriculture GA 214RW UT WOS:000324154500008 ER PT J AU Lewis, MA Trabelsi, S Nelson, SO Tollner, EW Haidekker, MA AF Lewis, M. A. Trabelsi, S. Nelson, S. O. Tollner, E. W. Haidekker, M. A. TI AN AUTOMATED APPROACH TO PEANUT DRYING WITH REAL-TIME MICROWAVE MONITORING OF IN-SHELL KERNEL MOISTURE CONTENT SO APPLIED ENGINEERING IN AGRICULTURE LA English DT Article DE Dielectric properties; Feedback controller; In-shell kernel moisture content; Microwave sensing; Peanut drying; Sensor network; Unshelled peanuts ID BULK-DENSITY AB Peanut (arachis hypogaea L.) drying is an essential task preceding the grading process. Kernel moisture is one of five parameters used to establish the grade for a specific lot of peanuts, and it is imperative that peanuts be dried to a kernel moisture content <10.5% wet basis for grading and storage purposes. Today's peanut drying processes utilize decision support software based on modeling and require substantial human interaction to obtain kernel moisture content. These conditions increase the likelihood of peanuts being overdried or underdried. This research addresses the need for an automated controller with real-time, in-shell kernel moisture content determination capabilities. By using a microwave inoisture meter, developed within United States Department of Agriculture, Agricultural Research Service, the moisture content of the peanut kernel can be determined without having to shell the peanut pods. The kernel moisture content and atmospheric conditions serve as inputs to the controller, and thus, air temperature and drying time are controlled automatically. Such implementation reduces overdrying and underdrying, preserves quality of peanuts, and minimizes energy consumption through efficient control of the heater In this article, a quarter-scale drying system with automated control is discussed Kernel moisture content was determined in real time with a standard error of performance of 0.55% moisture when compared to the reference oven-drying method Such results show promise for large-scale implementation and testing. C1 [Lewis, M. A.; Trabelsi, S.; Nelson, S. O.] USDA ARS, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. [Tollner, E. W.; Haidekker, M. A.] Univ Georgia, Dept Biol & Agr Engn, Athens, GA 30602 USA. RP Lewis, MA (reprint author), USDA ARS, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. EM micah.lewis@ars.usda.gov NR 31 TC 3 Z9 3 U1 1 U2 8 PU AMER SOC AGRICULTURAL & BIOLOGICAL ENGINEERS PI ST JOSEPH PA 2950 NILES RD, ST JOSEPH, MI 49085-9659 USA SN 0883-8542 J9 APPL ENG AGRIC JI Appl. Eng. Agric. PD JUL PY 2013 VL 29 IS 4 BP 583 EP 593 PG 11 WC Agricultural Engineering SC Agriculture GA 214RW UT WOS:000324154500016 ER PT J AU Johnson, EJ AF Johnson, E. J. TI LUTEIN AND ZEAXANTHIN: FROM THE RETINA TO THE BRAIN SO EUROPEAN JOURNAL OF OPHTHALMOLOGY LA English DT Meeting Abstract C1 [Johnson, E. J.] Tufts Univ, Carotenoids & Hlth Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU WICHTIG EDITORE PI MILAN PA 72/74 VIA FRIULI, 20135 MILAN, ITALY SN 1120-6721 J9 EUR J OPHTHALMOL JI Eur. J. Ophthalmol. PD JUL-AUG PY 2013 VL 23 IS 4 BP 604 EP 605 PG 2 WC Ophthalmology SC Ophthalmology GA 214RE UT WOS:000324152400029 ER PT J AU Mares, JA Meyers, KJ Johnson, EJ AF Mares, J. A. Meyers, K. J. Johnson, E. J. TI ENVIRONMENTAL AND GENETIC DETERMINANTS OF MACULAR PIGMENTS AND RISK FOR AGE-RELATED MACULAR DEGENERATION SO EUROPEAN JOURNAL OF OPHTHALMOLOGY LA English DT Meeting Abstract C1 [Mares, J. A.; Meyers, K. J.] Univ Wisconsin, Sch Med & Publ Hlth, Dept Ophthalmol & Visual Sci, Madison, WI USA. [Johnson, E. J.] Tufts Univ, Jean Mayer USDA HNRCA, Medford, MA 02155 USA. NR 0 TC 0 Z9 0 U1 0 U2 4 PU WICHTIG EDITORE PI MILAN PA 72/74 VIA FRIULI, 20135 MILAN, ITALY SN 1120-6721 J9 EUR J OPHTHALMOL JI Eur. J. Ophthalmol. PD JUL-AUG PY 2013 VL 23 IS 4 BP 606 EP 606 PG 1 WC Ophthalmology SC Ophthalmology GA 214RE UT WOS:000324152400036 ER PT J AU Vishwanathan, R Kuchan, MJ Johnson, EJ AF Vishwanathan, Rohini Kuchan, Matthew J. Johnson, Elizabeth J. TI LUTEIN IS THE PREDOMINANT CAROTENOID IN INFANT BRAIN SO EUROPEAN JOURNAL OF OPHTHALMOLOGY LA English DT Meeting Abstract C1 [Vishwanathan, Rohini] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Johnson, Elizabeth J.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Carotenoids & Hlth Lab, Boston, MA 02111 USA. [Kuchan, Matthew J.] Abbott Nutr, Columbus, OH USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU WICHTIG EDITORE PI MILAN PA 72/74 VIA FRIULI, 20135 MILAN, ITALY SN 1120-6721 J9 EUR J OPHTHALMOL JI Eur. J. Ophthalmol. PD JUL-AUG PY 2013 VL 23 IS 4 BP 609 EP 609 PG 1 WC Ophthalmology SC Ophthalmology GA 214RE UT WOS:000324152400049 ER PT J AU Tarin, D Pepper, AE Goolsby, JA Moran, PJ Arquieta, AC Kirk, AE Manhart, JR AF Tarin, Daniel Pepper, Alan E. Goolsby, John A. Moran, Patrick J. Contreras Arquieta, Alberto Kirk, Alan E. Manhart, James R. TI Microsatellites Uncover Multiple Introductions of Clonal Giant Reed (Arundo donax) SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Biological control; clonal; invasive species; Mediterranean; Rio Grande Basin; RGB ID MULTILOCUS GENOTYPE DATA; BIOLOGICAL-CONTROL; POPULATION-STRUCTURE; HEMIPTERA DIASPIDIDAE; GENETIC DIVERSITY; NORTH-AMERICA; MUTATION-RATE; HOST-RANGE; LOCI; L. AB Giant reed (Arundo donax) is an invasive weed that is native to the Old World. Tens of thousands of hectares of riparian habitat in the Rio Grande Basin (RGB) in Texas and Mexico have been heavily affected by invasions of Arundo. Additionally, many other watersheds across the southwestern United States have also been affected. Giant reed is being targeted for biological control because it displaces native vegetation and consumes water that could potentially be used for agricultural and municipal purposes, especially in areas with limited access to water. Finding the best-adapted insects for biological control involves locating the origin(s) of this plant. To narrow down the proximal source(s) of invasion of giant reed in the KGB, 10 microsatellite markers were developed. An analysis of 203 Old World and 159 North American plants, with an emphasis on the RGB, indicated a reduction in the allelic diversity in the introduced range compared with the Old World. Clonal assignment, neighbor joining, principal coordinates analyses, and STRUCTURE analyses were consistent and implied multiple introductions in North America, with one (likely clonal) lineage responsible for the invasion of the RGB, northern Mexico, and other parts of the southwestern United States. Although no identical matches with the RGB lineage were found in the Old World, several close matches were found on the Mediterranean coast of Spain. C1 [Tarin, Daniel; Pepper, Alan E.; Manhart, James R.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Goolsby, John A.; Moran, Patrick J.] ARS, USDA, Kika de la Garza Subtrop Agr Res Ctr, Weslaco, TX 78596 USA. [Contreras Arquieta, Alberto] Pronatura Norestre, Monterrey, Mexico. [Kirk, Alan E.] USDA ARS, European Biol Control Lab, Montpellier, France. RP Tarin, D (reprint author), Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. EM dtarin@bio.tamu.edu FU Department of Homeland Security, Customs and Border Protection; Science and Technology Directorate FX This research was funded by the Department of Homeland Security, Customs and Border Protection, and Science and Technology Directorate. We also acknowledge the Lower Rio Grande Valley Development Council (Ken Jones), Brownsville Irrigation District (Joe Barrera), San Benito Irrigation District (Sonja Lambert), and Hidalgo County Water District #2 (Sonny Hinojosa) for their early support on this project. Additionally, we acknowledge Borlaug Center, Laboratory for Genome Technology, Gene Technologies Laboratory, Gerhild Kirk (France), A. Zeddam (Algeria), Dan Gerling (Israel), Alex Racelis and Maricela Martinez Jimenez (Mexico), Ann Vacek, Crystal Salinas, John Adamczyk (USDA-ARS, Weslaco), Paul Parker, Matt Ciomperlik and Jo-Ann Bentz Blanco (USDA-APHIS), Avinoam Danin (Israel), Martin Terry. NR 55 TC 10 Z9 11 U1 3 U2 24 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2013 VL 6 IS 3 BP 328 EP 338 DI 10.1614/IPSM-D-12-00085.1 PG 11 WC Plant Sciences SC Plant Sciences GA 217GG UT WOS:000324346900002 ER PT J AU Rector, BG Ashley, MC Gaskin, JF Longland, WS AF Rector, Brian G. Ashley, Michael C. Gaskin, John F. Longland, William S. TI Use of Wheat SSRs to Assess Genetic Diversity in Medusahead (Taeniatherum caput-medusae) SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Great Basin; invasive species; molecular biology; SNP; SSR ID MULTILOCUS GENOTYPE DATA; TRITICEAE POACEAE; INFERENCE; MARKERS; HYBRIDIZATION; PROGRAMS; L. AB Medusahead is a close relative of bread wheat that is native to Eurasia but has become a noxious, invasive weed in North America. Intergeneric use of primers for bread wheat simple-sequence repeat (SSR) markers was tested in medusahead in order to expand the pool of available genetic resources for study of this plant. Forty-two primer pairs were screened in medusahead, of which 29 produced visible bands in agarose gels. Amplicons from eight of these markers were sequenced and analyzed for the presence of SSRs and single-nucleotide polymorphisms (SNPs) among medusahead individuals from six populations in the western Great Basin. Of the eight sequenced amplicons, two contained SSRs, both of which were polymorphic and shared by the original bread wheat marker. Six of the eight markers combined to detect 33 SNP loci. BLAST comparisons of the eight amplicons revealed variable numbers of matching sequences from wheat and other grass species ranging from 0 to > 200 matches. Using data from the polymorphic loci, population genetic analysis of the six invasive medusahead populations indicated that they arose from two separate introductions with two additional subdusters possible within the two principal clusters. Extrapolating from these results, it is reasonable to expect that between 170 and 830 of the approximately 1,200 publicly available bread wheat SSRs would produce useful marker loci in medusahead. C1 [Rector, Brian G.; Ashley, Michael C.; Longland, William S.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. [Gaskin, John F.] USDA ARS, Pest Management Res Unit, Sidney, MT 59270 USA. RP Rector, BG (reprint author), USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM brian.rector@ars.usda.gov NR 29 TC 0 Z9 0 U1 1 U2 8 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2013 VL 6 IS 3 BP 352 EP 361 DI 10.1614/IPSM-D-12-00087.1 PG 10 WC Plant Sciences SC Plant Sciences GA 217GG UT WOS:000324346900004 ER PT J AU DiTommaso, A Milbrath, LR Bittner, T Wesley, FR AF DiTommaso, Antonio Milbrath, Lindsey R. Bittner, Todd Wesley, F. Robert TI Pale Swallowwort (Vincetoxicum rossicum) Response to Cutting and Herbicides SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Dog-strangling vine; chemical control; forest understory; integrated weed management; invasive plant; mechanical control; natural areas; old field; vine ID NEW-YORK-STATE; LIGHT ENVIRONMENTS; NORTH-AMERICA; V. NIGRUM; WORT; GROWTH; VINE; ASCLEPIADACEAE; SURVIVAL; BIOLOGY AB Effective control techniques for pale swallowwort (PSW), an invasive herbaceous vine of old fields and forest understories, are limited. We conducted a 3-yr cutting and herbicide study on an adjacent old-field and forest understory site near Ithaca, NY, for control of PSW. Plants in experimental plots were cut in early July and cut again. or sprayed in late August for two seasons with the isopropylamine salt of glyphosate, or one of two rates (low or high) of either tridopyr triethylamine salt (i.e., SL, SH) or tridopyr butoxyethyl ester (EL, EH). The herbicide treatments were effective in reducing PSW cover, plant (stem) density, and aboveground biomass in the old-field site, but in several cases, only after 2 yr of cutting plus herbicide application. Only the cutting plus SH treatment did not reduce PSW cover relative to the unmanaged control in the forest understory and no treatment reduced biomass. In general, the cutting plus EH treatment was most effective in reducing PSW stem densities in the forest site. The most effective herbicide treatments differed between sites. Cutting plus EH reduced PSW cover by 84% and stem density (> 5 cm) by 86% in the old-field site. Cutting plus SH effectively decreased long and short (<= 5 cm) stem densities by 86 and 96%, respectively. Cutting plants twice during each of two seasons increased PSW cover by 301% and density of stems > 5 cm by 73% at this site. In the forest site, cutting plus glyphosate, or cutting plus EH or cutting plus SL and EL resulted in the greatest reductions in PSW cover (80, 76, 66, and 56%, respectively). Cover in plots cut twice per year decreased by 19%. The EH or SL treatments decreased long-stem densities by 78 and 71%, respectively. The EH treatment decreased short-stem density by 37%. These findings suggest that integrated techniques may control PSW but that effective management strategies may be habitat constrained. C1 [DiTommaso, Antonio] Cornell Univ, Dept Crop & Soil Sci, Ithaca, NY 14853 USA. [Milbrath, Lindsey R.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Bittner, Todd; Wesley, F. Robert] Cornell Univ, Cornell Plantat, Ithaca, NY 14853 USA. RP DiTommaso, A (reprint author), Cornell Univ, Dept Crop & Soil Sci, Ithaca, NY 14853 USA. EM ad97@cornell.edu FU U.S. Department of Agriculture (USDA)-Agricultural Research Service project [1907-22620-003-01S] FX We thank Scott Morris (Cornell) for assistance with all field operations. We thank Sandy Bonanno and George Spak for carrying out the herbicide applications. Thanks also to Jeromy Biazzo (USDA-ARS) and several undergraduate students for their assistance with data collection. We thank Joe Buttafuoco for help setting up the plots. We are grateful to Elizabeth Buck for help with early versions of the manuscript. Financial support was provided by the U.S. Department of Agriculture (USDA)-Agricultural Research Service project 1907-22620-003-01S. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 29 TC 5 Z9 5 U1 1 U2 32 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2013 VL 6 IS 3 BP 381 EP 390 DI 10.1614/IPSM-D-12-00078.1 PG 10 WC Plant Sciences SC Plant Sciences GA 217GG UT WOS:000324346900007 ER PT J AU Blank, RR Morgan, T AF Blank, Robert R. Morgan, Tye TI Soil Engineering Facilitates Downy Brome (Bromus tectorum) Growth - A Case Study SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Cheatgrass; Invasive weeds; plant competition; plant-soil relationships; soil fertility ID ALTERS NITROGEN DYNAMICS; PLANT INVASION; ECOSYSTEM ENGINEERS; COMMUNITY STRUCTURE; NUTRIENT DYNAMICS; ARID GRASSLAND; EVOLUTION; ALIEN; INVASIVENESS; RESPONSES AB Soil engineering by downy brome may be a facet of its competitiveness. Using rhizotrons in the greenhouse, we compared the growth and plant-soil relationships of downy brome grown in two field soil types: soil invaded for 12 yr by downy brome and a similar soil not yet invaded. For each soil type, downy brome was grown for two growth cycles. At harvest, root mass and soils were sampled at depths of 10, 40, and 80 cm (4, 16, and 32 in); aboveground biomass was also sampled. After the first growth cycle, downy brome grown in invaded soil had 250% greater aboveground biomass and nearly double the root mass per soil volume at 10 cm relative to downy brome grown in noninvaded soil; root mass per volume was similar at depths of 40 and 80 cm. For the second growth cycle, aboveground biomass declined, but was twice greater for downy brome grown in invaded soil; however, root mass per volume was similar between soil types for each depth. Soil attributes that positively related to aboveground biomass included bicarbonate-extractable P, DTPA (diethylentriamene pentaacetate)-extractable Mn, and solution-phase SC42- (80-cm depth). We conclude that the data support our hypothesis that downy brome has engineered the soil to increase its growth potential, but proof will require a more robust experimental design. Plant competition is affected by myriad interactions; however, a plant that can increase the availability of soil nutrients for itself and its growth potential, relative to competing plants, would appear to be at an advantage. The mechanistic underpinnings involved are inconclusive, but may involve increased availability of soil N, P, and Mn. C1 [Blank, Robert R.; Morgan, Tye] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. RP Blank, RR (reprint author), USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM bob.blank@ars.usda.gov NR 58 TC 5 Z9 5 U1 3 U2 21 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2013 VL 6 IS 3 BP 391 EP 400 DI 10.1614/IPSM-D-12-00086.1 PG 10 WC Plant Sciences SC Plant Sciences GA 217GG UT WOS:000324346900008 ER PT J AU Lemke, D Schweitzer, CJ Tadesse, W Wang, Y Brown, JA AF Lemke, Dawn Schweitzer, Callie J. Tadesse, Wubishet Wang, Yong Brown, Jennifer A. TI Geospatial Assessment of Invasive Plants on Reclaimed Mines in Alabama SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Invasive plants; logistic regression; maximum entropy; remote sensing; spatial analysis ID UNITED-STATES; SPATIAL HETEROGENEITY; SURFACE MINES; GLOBAL CHANGE; DISTRIBUTIONS; LANDSCAPE; FOREST; USA; FEATURES; ECOLOGY AB Throughout the world, the invasion of nonnative plants is an increasing threat to native biodiversity and ecosystem sustainability. Invasion is especially prevalent in areas affected by land transformation and disturbance. Surface mines are a major land transformation, and thus may promote the establishment and persistence of invasive plant communities. Using the Shale Hills region of Alabama as a case study, we assessed the use of landscape characteristics in predicting the probability of occurrence of six invasive plant species: sericea lespedeza, Japanese honeysuckle, Chinese privet, autumn-olive, royal paulownia, and sawtooth oak. Models were generated for invasive species occurrence using logistic regression and maximum entropy methods. The predicted probabilities of species occurrence were applied to the mined landscape to assess the probable prevalence of each species across the landscape. Japanese honeysuckle had the highest probable prevalence on the landscape (48% of the area), with royal paulownia having the lowest (less than 1%). Overall, 67% of the landscape was predicted to have at least one invasive plant species, with 20% of the landscape predicted to have two or more species, and 3% of the landscape predicted to have three or more species. Japanese honeysuckle, sericea lespedeza, privet, and autumn-olive showed higher occurrence on the reclaimed sites than across the broader region. We found that geospatial modeling of these invasive plants at this scale offered potential for management, both for identifying habitat types at risk and areas that need management attention. However, the most immediate action for reducing the prevalence of invasive plants on reclaimed mines is to remove invasive plants from the reclamation planting list. Three (sericea lespedeza, autumn-olive, and sawtooth oak) out of the six most common invasive plants in this study were planted as part of reclamation activities. C1 [Lemke, Dawn; Brown, Jennifer A.] Univ Canterbury, Dept Math & Stat, Christchurch 8140, New Zealand. [Lemke, Dawn; Tadesse, Wubishet; Wang, Yong] Alabama A&M Univ, Dept Biol & Environm Sci, Normal, AL 35762 USA. [Schweitzer, Callie J.] US Forest Serv, Southern Res Stn, USDA, Normal, AL 35762 USA. RP Lemke, D (reprint author), Univ Canterbury, Dept Math & Stat, Private Bag 4800, Christchurch 8140, New Zealand. EM dawn.lemke@aamu.edu FU U.S. Office of Surface Mining [S09AC15438] FX We wish to thank the U.S. Office of Surface Mining for supporting this work (OSM cooperative agreement S09AC15438). Also we would like to thank Shelly Baltar (Alabama A&M University [AAMU]), Matthew Carr (USFS), Kathleen Roberts (AAMU), Ryan Sisk (USFS), Dana Virone (AAMU), and Matthew Zirbel (AAMU) for their assistance in the field and with data processing and manuscript review. AAMU paper number 652. NR 50 TC 6 Z9 6 U1 2 U2 36 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2013 VL 6 IS 3 BP 401 EP 410 DI 10.1614/IPSM-D-12-00045.1 PG 10 WC Plant Sciences SC Plant Sciences GA 217GG UT WOS:000324346900009 ER PT J AU McGlone, CM AF McGlone, Christopher M. TI No Long-Term Effects of Prescribed Fire on Lehmann Lovegrass (Eragrostis lehmanniana)-Invaded Desert Grassland SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Black grama; invasive species; New Mexico; perennial grasses; prescribed burn; shrub encroachment ID NORTHERN CHIHUAHUAN DESERT; ERAGROSTIS-LEHMANNIANA; PROSOPIS-GLANDULOSA; ARIZONA GRASSLAND; GRASSES; VEGETATION; RESPONSES; DISTURBANCE; INTENSITY; INVASIONS AB Desert grasslands of the southwestern United States have experienced an increase in the abundance and distribution of woody plant species over the past century. Shrub encroachment has caused a substantial loss of grasslands in the northern Chihuahuan Desert. The Chihuahuan Desert has also been invaded by Lehmann lovegrass, a fire-adapted species from southern Africa. In 1999, the U.S. Department of Agriculture Agricultural Research Service burned a remnant desert grassland to determine the effects of prescribed fire on shrub-perennial grass dynamics. The grassland also contained the nonnative perennial grass Lehmann lovegrass. I am reporting on a study that was initiated to determine whether prescribed burning would alter the vegetative community within and proximal to a Lehmann lovegrass-dominated patch. Cover of Lehmann lovegrass showed no significant response to the burn treatment. Of the dominant native species, only black grama and broom snakeweed had a significant year by treatment interaction. No species or growth form had a significant vegetation type by year by treatment interaction. After 6 yr, differences between burned and unburned transects were not significant for any species or growth form. C1 [McGlone, Christopher M.] New Mexico State Univ, Jornada Basin LTER, Las Cruces, NM 88003 USA. RP McGlone, CM (reprint author), Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. EM Chris.McGlone@nau.edu FU Jornada Long-Term Ecological Research Project (NSF) [DEB 94-111971, DEB 00-80412] FX This study was funded and supported by the Jornada Long-Term Ecological Research Project (NSF grants DEB 94-111971 and DEB 00-80412). The author thanks J. Anderson, T. Flores, J. Herrick, L. Huenneke, J. Lenz, L. Murray, B. Nolan, D. Peters, and D. Thatcher for their support throughout the course of this study. J. Anderson and D. Peters also gave useful feedback on earlier versions of this manuscript. Additionally, two anonymous reviewers gave excellent feedback that substantially improved the quality of this manuscript. I also thank the USDA-ARS for allowing me to conduct this experiment on the JER. NR 33 TC 0 Z9 0 U1 5 U2 21 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD JUL-SEP PY 2013 VL 6 IS 3 BP 449 EP 456 DI 10.1614/IPSM-D-12-00059.1 PG 8 WC Plant Sciences SC Plant Sciences GA 217GG UT WOS:000324346900014 ER PT J AU Stika, JA AF Stika, Jon A. TI Addressing some hurdles to implementing soil health management systems SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material C1 USDA, Nat Resources Conservat Serv, Dickinson, ND USA. RP Stika, JA (reprint author), USDA, Nat Resources Conservat Serv, Dickinson, ND USA. NR 0 TC 1 Z9 1 U1 3 U2 8 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 99A EP 99A DI 10.2489/jswc.68.4.99A PG 1 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900003 ER PT J AU Hu, LJ Flanagan, DC AF Hu, Liang-Jun Flanagan, Dennis C. TI Towards new-generation soil erosion modeling: Building a unified omnivorous model SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material ID WATER EROSION; LOSS EQUATION; CLIMATE-CHANGE; PREDICTION; WIND; SCALE; TECHNOLOGY; LISEM C1 [Hu, Liang-Jun] NE Normal Univ, Chinese Minist Educ, Key Lab Vegetat Ecol Sci, Changchun, Jilin, Peoples R China. [Flanagan, Dennis C.] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Hu, LJ (reprint author), NE Normal Univ, Chinese Minist Educ, Key Lab Vegetat Ecol Sci, Changchun, Jilin, Peoples R China. NR 44 TC 0 Z9 0 U1 2 U2 13 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 100A EP 103A DI 10.2489/jswc.68.4.100A PG 4 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900004 ER PT J AU Liu, BL Qu, JJ Wagner, LE AF Liu, Benli Qu, Jianjun Wagner, Larry E. TI Building Chinese wind data for Wind Erosion Prediction System using surrogate US data SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material C1 [Liu, Benli; Qu, Jianjun] Chinese Acad Sci, Dunhuang Gobi Desert Ecol & Environm Res Stn, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou, Gansu, Peoples R China. [Wagner, Larry E.] USDA ARS, Engn & Wind Eros Res Unit, Ctr Grain & Anim Hlth, Manhattan, KS USA. RP Liu, BL (reprint author), Chinese Acad Sci, Dunhuang Gobi Desert Ecol & Environm Res Stn, Cold & Arid Reg Environm & Engn Res Inst, Lanzhou, Gansu, Peoples R China. OI Liu, Benli/0000-0002-7664-5142 NR 16 TC 4 Z9 4 U1 0 U2 9 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 104A EP 107A DI 10.2489/jswc.68.4.104A PG 4 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900005 ER PT J AU Kome, CE Andrews, SS Franzluebbers, AJ AF Kome, C. E. Andrews, S. S. Franzluebbers, A. J. TI Soil organic carbon assessment using the Carbon Management Evaluation Tool for Voluntary Reporting and the Soil Conditioning Index SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE conservation tillage; crop rotation; modeling; soil organic matter; soil texture ID GREENHOUSE-GAS CONTRIBUTIONS; SIMULATING TRENDS; MICROBIAL BIOMASS; CROPPING INTENSITY; UNITED-STATES; GREAT-PLAINS; AGRICULTURAL MANAGEMENT; DIFFERENT TEXTURE; SOUTHEASTERN USA; CLAY CONTENT AB Simple, yet reliable models are needed to quantify soil organic carbon (SOC) changes for the wide diversity of agricultural management conditions in the United States. We compared the outputs of two relatively simple models currently available for farmers and government-financed farm support agencies: the Carbon (C) Management Evaluation Tool for Voluntary Reporting of Greenhouse Gases (COMET-VR) and the Soil Conditioning Index (SCI). Simulations were conducted for 18 locations throughout the United States for five soil textural regimes (loamy sand, sandy loam, silt loam, clay loam, and silty clay loam), three tillage management systems (conventional tillage [CT], minimum tillage [MT], and no tillage [NT]), and two crop rotations (wheat [Triticum aestivum L.]-potato [Solanum tuberosum L.] and wheat-four-year alfalfa [Medicago sativa L.] in western states and corn [Zea mays L.]-soybean [Glycine max L.] and corn-soybean-wheat in eastern states). Both models ranked SOC change as NT > MT > CT, whereby SOC change decreased with increasing soil disturbance with tillage However, models were divergent with regards to soil texture; SOC change was greater in coarse-textured than in fine-textured soils with COMET-VR, but SOC change was lower in coarse-textured than in fine-textured soils with SCI. For crop rotations, SOC change was greater or equal in simpler than in more complex rotation with COMET-VR, but smaller in simpler than in more complex rotations with SCI. Overall, SOC sequestration predicted by COMET-VR was positively related to SCI score, especially when accounting for differences in environmental conditions of a location. Our results suggest that both Models have value and limitations and that measures of SOC sequestration are predictable with these tools under a diversity of typical management conditions in the United States. C1 [Kome, C. E.] Nat Resources Conservat Serv, USDA, Greensboro, NC USA. [Andrews, S. S.] Nat Resources Conservat Serv, USDA, Lincoln, NE USA. [Franzluebbers, A. J.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. RP Kome, CE (reprint author), Nat Resources Conservat Serv, USDA, Greensboro, NC USA. NR 73 TC 1 Z9 1 U1 1 U2 18 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 296 EP 305 DI 10.2489/jswc.68.4.296 PG 10 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900009 ER PT J AU Myers, TL Dell, CJ Beegle, DB AF Myers, T. L. Dell, C. J. Beegle, D. B. TI Evaluation of ammonia emissions from manure incorporated with different soil aerator configurations SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE ammonia; incorporation; manure; soil aeration ID CORN YIELD RESPONSE; LOW-DISTURBANCE; NITROGEN-FERTILIZER; VOLATILIZATION; CONSERVATION; VARIABILITY; COMPACTION; TILLAGE; IMPACTS; SILAGE AB The incorporation of swine manure with tillage effectively reduces ammonia (NH3) emissions and conserves crop available nitrogen (N) but is not compatible with no-till and many other conservation tillage programs. Rolling-tine aerators potentially provide a means to enhance manure infiltration with limited disruption of the soil surface; however, the impact of the range of aerator configurations and manure placements has not been widely studied. We measured NH3 emissions, conducted presidedress nitrate (NO3) tests (PSNT), monitored corn (Zea mays L.) grain yield when swine manure was incorporated with combinations of two aerator toolbar offset angles (0 degrees and 10 degrees) and two manure placements (broadcast before aeration and banded behind aerator), and compared those measurements to unincorporated surface application and an unmanured control. Additional plots were established with five rates of mineral N fertilization to determine N response curve and estimate fertilizer N equivalent of the applied manure. Measurement of cumulative NH3 emissions using a dynamic chamber method showed that offsetting the aerator toolbar by 10 degrees reduced emissions by 65% to 75%, with both manure placements, compared to surface application. However, no consistent reductions in NH3 emissions were observed when the toolbar was not offset. While reduction in NH3 with the offset toolbar could conserve substantial amounts of plant available N, the soil disturbance caused by the offset tines would not be compatible with most conservation tillage programs. Presidedress NO3 test values increased when NH3 emissions were reduced. In 2009, both the angled and straight aerator treatments with manure banded behind the aerator resulted in significantly higher corn grain yield than all other treatments despite reductions in NH3 emissions only when the toolbar was angled. Correlation of yield from manure application treatments with the N response curve for multiple mineral N application rates showed a greater than expected response to manure application.Yield responses are an indication that aeration has benefits in addition to N conservation. We do not recommend the rolling-tine aerator if reducing NH3 emissions in conservation tillage is the primary goal, but future study into other yield-increasing benefits of aeration is needed. C1 [Myers, T. L.] Penn State Univ, Dept Crop & Soil Sci, University Pk, PA 16802 USA. [Dell, C. J.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA USA. [Beegle, D. B.] Penn State Univ, University Pk, PA 16802 USA. RP Myers, TL (reprint author), Nat Resources Conservat Serv, USDA, Chambersburg, PA USA. FU Pennsylvania Pork Producers Council FX The authors thank the Pennsylvania Pork Producers Council for providing the funding which supported this research. We are also thankful for the invaluable assistance provided by the staff of the Penn State College of Agricultural Sciences and the USDA Agricultural Research Service Pasture Systems and Watershed Management Unit (ARS PSWMRU), especially Justin Dillon, senior extension associate, Plant Sciences Department, Penn State University; Bart Moyer, chemist (retired), USDA ARS PSWMRU; Lou Saporito, soil scientist, USDA ARS PSWMRU; Sarah Fishel, biological science technician, USDA ARS PSWMRU; and Mary Kay Lupton, project assistant, Ecosystems Science and Management Department, Penn State University All are located in University Park, Pennsylvania. NR 33 TC 1 Z9 1 U1 4 U2 8 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 306 EP 314 DI 10.2489/jswc.68.4.306 PG 9 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900010 ER PT J AU Biswas, S Shapiro, CA Kranz, WL Mader, TL Shelton, DP Snow, DD Bartelt-Hunt, SL Tarkalson, DD van Donk, SJ Zhang, TC Ensley, S AF Biswas, S. Shapiro, C. A. Kranz, W. L. Mader, T. L. Shelton, D. P. Snow, D. D. Bartelt-Hunt, S. L. Tarkalson, D. D. van Donk, S. J. Zhang, T. C. Ensley, S. TI Current knowledge on the environmental fate, potential impact, and management of growth-promoting steroids used in the US beef cattle industry SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material ID ENDOCRINE-DISRUPTING CHEMICALS; ANIMAL FEEDING OPERATIONS; WASTE-WATER CONTAMINANTS; 17 BETA-ESTRADIOL; AGRICULTURAL SOILS; ESTROGENIC HORMONES; FATHEAD MINNOW; MELENGESTROL ACETATE; PIMEPHALES-PROMELAS; TRENBOLONE ACETATE C1 [Biswas, S.; Shapiro, C. A.; Kranz, W. L.] Univ Nebraska Lincoln, Dept Biol Syst Engn, Concord, NE USA. [Shapiro, C. A.] Univ Nebraska Lincoln, Dept Agron & Hort, Concord, NE USA. [Mader, T. L.] Univ Nebraska Lincoln, Dept Anim Sci, Concord, NE USA. [Snow, D. D.] Univ Nebraska, Water Sci Lab, Lincoln, NE USA. [Bartelt-Hunt, S. L.] Univ Nebraska, Dept Civil Engn, Omaha, NE 68182 USA. [Tarkalson, D. D.] ARS, USDA, Kimberly, ID USA. [van Donk, S. J.] Univ Nebraska Lincoln, Dept Biol Syst Engn, North Platte, NE USA. [Zhang, T. C.] Univ Nebraska, Dept Civil Engn, Lincoln, NE 68588 USA. [Ensley, S.] Iowa State Univ, Coll Vet Med, Ames, IA USA. RP Biswas, S (reprint author), Univ Nebraska Lincoln, Dept Biol Syst Engn, Concord, NE USA. NR 116 TC 8 Z9 8 U1 1 U2 40 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 325 EP 336 DI 10.2489/jswc.68.4.325 PG 12 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900012 ER PT J AU Gold, AJ Parker, D Waskom, RM Dobrowolski, J O'Neill, M Groffman, PM Addy, K Barber, M Batie, S Benham, B Bianchi, M Blewett, T Evensen, C Farrell-Poe, K Gardner, C Graham, W Harrison, J Harter, T Kushner, J Lowrance, R Lund, J Mahler, R McClaran, M McFarland, M Osmond, D Pritchett, J Prokopy, L Rock, C Shober, A Silitonga, M Swackhather, D Thurston, J Todey, D Turco, R Vellidis, G Morton, LW AF Gold, A. J. Parker, D. Waskom, R. M. Dobrowolski, J. O'Neill, M. Groffman, P. M. Addy, K. Barber, M. Batie, S. Benham, B. Bianchi, M. Blewett, T. Evensen, C. Farrell-Poe, K. Gardner, C. Graham, W. Harrison, J. Harter, T. Kushner, J. Lowrance, R. Lund, J. Mahler, R. McClaran, M. McFarland, M. Osmond, D. Pritchett, J. Prokopy, L. Rock, C. Shober, A. Silitonga, M. Swackhather, D. Thurston, J. Todey, D. Turco, R. Vellidis, G. Morton, L. Wright TI Advancing water resource management in agricultural, rural, and urbanizing watersheds: Why land-grant universities matter SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material ID UNITED-STATES; CLIMATE-CHANGE; GROUNDWATER-MANAGEMENT; QUALITY MANAGEMENT; NITROGEN; AQUIFER; USA; SUSTAINABILITY; CONSERVATION; DEPLETION C1 [Gold, A. J.] Univ Rhode Isl, Dept Nat Resources Sci, Kingston, RI 02881 USA. [Parker, D.] Univ Califf, Califf Inst Water Resources, Oakland, CA USA. [Waskom, R. M.] Colorado State Univ, Colorado Water Inst, Water Ctr, Ft Collins, CO 80523 USA. [Dobrowolski, J.] Natl Inst Food & Agr, USDA, Washington, DC USA. [O'Neill, M.] Univ Connecticut, Cooperat Extens Syst, Storrs, CT USA. [Groffman, P. M.] Cary Inst Ecosyst Studies, Millbrook, NY USA. [Addy, K.] Univ Rhode Isl, Coll Environm & Life Sci, Kingston, RI 02881 USA. [Barber, M.] Washington State Univ, Dept Civil & Environm Engn, Pullman, WA 99164 USA. [Batie, S.] Michigan State Univ, Dept Agr & Resource Econ, E Lansing, MI 48824 USA. [Benham, B.] Virginia Polytech Inst & State Univ, Dept Biol Syst Engn, Blacksburg, VA USA. [Bianchi, M.] Univ Califf Cooperat Extens, San Luis Obispo, CA 93407 USA. [Blewett, T.] Univ Wisconsin, Dept Engn Profess Dev, Madison, WI USA. [Evensen, C.] Univ Hawaii, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Farrell-Poe, K.] Univ Arizona, Biosyst Engn Dept, Tucson, AZ USA. [Gardner, C.] Florida A&M Univ, Tallahassee, FL 32307 USA. [Graham, W.] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL USA. [Graham, W.] Univ Florida, Water Inst, Gainesville, FL USA. [Harrison, J.] Washington State Univ, Pullman, WA 99164 USA. [Harter, T.] Univ Calif Davis, Davis, CA 95616 USA. [Kushner, J.] Univ Wisconsin, Madison, WI USA. [Lowrance, R.] ARS, USDA, Tifton, GA USA. [Lund, J.] Univ Calif Davis, Ctr Watershed Sci, Davis, CA 95616 USA. [Mahler, R.] Univ Idaho, Environm Sci Program, Moscow, ID 83843 USA. [McClaran, M.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [McFarland, M.] Texas A&M Univ, College Stn, TX USA. [Osmond, D.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Pritchett, J.] Colorado State Univ, Dept Agr & Resource Econ, Ft Collins, CO 80523 USA. [Prokopy, L.] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Rock, C.] Univ Arizona, Maricopa, AZ USA. [Shober, A.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19717 USA. [Silitonga, M.] Kentucky State Univ, Frankfort, KY USA. [Swackhather, D.] Univ Minnesota, St Paul, MN 55108 USA. [Thurston, J.] Natl Inst Food & Agr, USDA, Washington, DC USA. [Todey, D.] S Dakota State Univ, Brookings, SD 57007 USA. [Turco, R.] Purdue Univ, W Lafayette, IN 47907 USA. [Vellidis, G.] Univ Georgia, Dept Crop & Soil Sci, Tifton, GA USA. [Morton, L. Wright] Iowa State Univ, Dept Sociol, Ames, IA USA. RP Gold, AJ (reprint author), Univ Rhode Isl, Dept Nat Resources Sci, Kingston, RI 02881 USA. RI Turco, Ronald/B-8739-2008 OI Turco, Ronald/0000-0002-1794-1486 NR 85 TC 7 Z9 7 U1 1 U2 25 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JUL-AUG PY 2013 VL 68 IS 4 BP 337 EP 348 DI 10.2489/jswc.68.4.337 PG 12 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA 180NW UT WOS:000321602900013 ER PT J AU Headlee, WL Hall, RB Zalesny, RS AF Headlee, William L. Hall, Richard B. Zalesny, Ronald S., Jr. TI Establishment of Alleycropped Hybrid Aspen "Crandon" in Central Iowa, USA: Effects of Topographic Position and Fertilizer Rate on Aboveground Biomass Production and Allocation SO SUSTAINABILITY LA English DT Article DE agroforestry; biofuels; marginal land; Populus; triticale; woody biomass ID MIDWESTERN UNITED-STATES; ALLEY CROPPING SYSTEMS; INTERCROPPING SYSTEM; TREE COMPETITION; SOUTHERN ONTARIO; WOODY BIOMASS; ENERGY CROPS; GROWTH; CLONES; AGROFORESTRY AB Hybrid poplars have demonstrated high productivity as short rotation woody crops (SRWC) in the Midwest USA, and the hybrid aspen "Crandon" (Populus alba L. x P. grandidenta Michx.) has exhibited particularly promising yields on marginal lands. However, a key obstacle for wider deployment is the lack of economic returns early in the rotation. Alleycropping has the potential to address this issue, especially when paired with crops such as winter triticale which complete their growth cycle early in the summer and therefore are expected to exert minimal competition on establishing trees. In addition, well-placed fertilizer in low rates at planting has the potential to improve tree establishment and shorten the rotation, which is also economically desirable. To test the potential productivity of "Crandon" alleycropped with winter triticale, plots were established on five topographic positions with four different rates of fertilizer placed in the planting hole. Trees were then harvested from the plots after each of the first three growing seasons. Fertilization resulted in significant increases in branch, stem, and total aboveground biomass across all years, whereas the effects of topographic position varied by year. Allocation between branches and stems was found to be primarily a function of total aboveground biomass. C1 [Headlee, William L.; Hall, Richard B.] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Zalesny, Ronald S., Jr.] US Forest Serv, Inst Appl Ecosyst Studies, No Res Stn, Rhinelander, WI 54501 USA. RP Headlee, WL (reprint author), Iowa State Univ, Dept Nat Resource Ecol & Management, 339 Sci 2, Ames, IA 50011 USA. EM wheadlee@iastate.edu; rbhall@iastate.edu; rzalesny@fs.fed.us FU ISU College of Agriculture and Life Sciences; Leopold Center for Sustainable Agriculture; USDA Agriculture and Food Research Initiative; US Forest Service Institute for Applied Ecosystem Studies FX Support and funding for this project were provided by ISU College of Agriculture and Life Sciences, Leopold Center for Sustainable Agriculture, USDA Agriculture and Food Research Initiative, and US Forest Service Institute for Applied Ecosystem Studies. In addition, a portion of the long-term study trees were provided by ArborGen, Inc. (Ridgeville, SC, USA). The authors would like to thank Steve Jungst, Phil Dixon, and Ken Moore of Iowa State University for reviewing earlier drafts of the manuscript, and Theo Gunther for his expertise in triticale management. NR 35 TC 3 Z9 3 U1 0 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD JUL PY 2013 VL 5 IS 7 BP 2874 EP 2886 DI 10.3390/su5072874 PG 13 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA 213IO UT WOS:000324049900003 ER PT J AU Hafla, AN MacAdam, JW Soder, KJ AF Hafla, Aimee N. MacAdam, Jennifer W. Soder, Kathy J. TI Sustainability of US Organic Beef and Dairy Production Systems: Soil, Plant and Cattle Interactions SO SUSTAINABILITY LA English DT Review DE organic agriculture; non-organic agriculture; organic pasture rule; dairy production; beef production; grass; legume; soil ID CONJUGATED LINOLEIC-ACID; STOCKER GROWTH-RATE; POLYUNSATURATED FATTY-ACIDS; GREENHOUSE-GAS EMISSIONS; LIFE-CYCLE ASSESSMENT; LONG-TERM CHANGES; MILK-FAT; CARCASS CHARACTERISTICS; NUTRITIONAL QUALITY; FINISHING SYSTEM AB In 2010, the National Organic Program implemented a rule for the US stating that pasture must be a significant source of feed in organic ruminant systems. This article will focus on how the pasture rule has impacted the management, economics and nutritional value of products derived from organic ruminant systems and the interactions of grazing cattle with pasture forages and soils. The use of synthetic fertilizers is prohibited in organic systems; therefore, producers must rely on animal manures, compost and cover crops to increase and maintain soil nitrogen content. Rotational and strip grazing are two of the most common grazing management practices utilized in grazing ruminant production systems; however, these practices are not exclusive to organic livestock producers. For dairy cattle, grazing reduces foot and leg problems common in confinement systems, but lowers milk production and exposes cows to parasites that can be difficult to treat without pharmaceuticals. Organic beef cattle may still be finished in feedlots for no more than 120 days in the US, but without growth hormones and antibiotics, gains may be reduced and illnesses increased. Grazing reduces the use of environmentally and economically costly concentrate feeds and recycles nutrients back to the soil efficiently, but lowers the rate of beef liveweight gain. Increased use of pasture can be economically, environmentally and socially sustainable if forage use efficiency is high and US consumers continue to pay a premium for organic beef and dairy products. C1 [Hafla, Aimee N.; Soder, Kathy J.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [MacAdam, Jennifer W.] Utah State Univ, Dept Plants Soils & Climate, Logan, UT 84322 USA. RP MacAdam, JW (reprint author), Utah State Univ, Dept Plants Soils & Climate, 4820 Old Main Hill, Logan, UT 84322 USA. EM aimee.hafla@ars.usda.gov; jennifer.macadam@usu.edu; kathy.soder@ars.usda.gov RI MacAdam, Jennifer/B-3156-2009 OI MacAdam, Jennifer/0000-0003-2349-9863 NR 130 TC 8 Z9 8 U1 6 U2 114 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD JUL PY 2013 VL 5 IS 7 BP 3009 EP 3034 DI 10.3390/su5073009 PG 26 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA 213IO UT WOS:000324049900012 ER PT J AU Carr, PM Gramig, GG Liebig, MA AF Carr, Patrick M. Gramig, Greta G. Liebig, Mark A. TI Impacts of Organic Zero Tillage Systems on Crops, Weeds, and Soil Quality SO SUSTAINABILITY LA English DT Review DE organic farming; biological farming; ecological agriculture; conservation tillage; no-till; cover crops; soil quality; weeds ID NORTHERN GREAT-PLAINS; KILLING COVER CROPS; CROPPING SYSTEMS; NO-TILL; CONSERVATION TILLAGE; REDUCED-TILLAGE; AGRICULTURAL MANAGEMENT; MICROBIAL BIOMASS; ROLLER-CRIMPER; SEED PREDATION AB Organic farming has been identified as promoting soil quality even though tillage is used for weed suppression. Adopting zero tillage and other conservation tillage practices can enhance soil quality in cropping systems where synthetic agri-chemicals are relied on for crop nutrition and weed control. Attempts have been made to eliminate tillage completely when growing several field crops organically. Vegetative mulch produced by killed cover crops in organic zero tillage systems can suppress annual weeds, but large amounts are needed for adequate early season weed control. Established perennial weeds are not controlled by cover crop mulch. Integrated weed management strategies that include other cultural as well as biological and mechanical controls have potential and need to be incorporated into organic zero tillage research efforts. Market crop performance in organic zero tillage systems has been mixed because of weed, nutrient cycling, and other problems that still must be solved. Soil quality benefits have been demonstrated in comparisons between organic conservation tillage and inversion tillage systems, but studies that include zero tillage treatments are lacking. Research is needed which identifies agronomic strategies for optimum market crop performance, acceptable levels of weed suppression, and soil quality benefits following adoption of organic zero tillage. C1 [Carr, Patrick M.] N Dakota State Univ, Dickinson Res Extens Ctr, Dickinson, ND 58601 USA. [Gramig, Greta G.] N Dakota State Univ, Fargo, ND 58108 USA. [Liebig, Mark A.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58544 USA. RP Carr, PM (reprint author), N Dakota State Univ, Dickinson Res Extens Ctr, 1041 State Ave, Dickinson, ND 58601 USA. EM Patrick.Carr.1@ndsu.edu; Greta.Gramig@ndsu.edu; mark.liebig@ars.usda.gov NR 151 TC 13 Z9 14 U1 15 U2 126 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2071-1050 J9 SUSTAINABILITY-BASEL JI Sustainability PD JUL PY 2013 VL 5 IS 7 BP 3172 EP 3201 DI 10.3390/su5073172 PG 30 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Environmental Sciences; Environmental Studies SC Science & Technology - Other Topics; Environmental Sciences & Ecology GA 213IO UT WOS:000324049900021 ER PT J AU Stoleson, SH AF Stoleson, Scott H. TI CONDITION VARIES WITH HABITAT CHOICE IN POSTBREEDING FOREST BIRDS SO AUK LA English DT Article DE body condition; clearcut; early-successional forest; habitat choice; mature-forest birds; postbreeding period ID OVENBIRDS SEIURUS-AUROCAPILLA; EARLY-SUCCESSIONAL FORESTS; EASTERN UNITED-STATES; POSTFLEDGING PERIOD; BODY CONDITION; REGENERATING CLEARCUTS; HOME-RANGE; MOVEMENTS; SONGBIRDS; SURVIVAL AB Many birds that are experiencing population declines require extensive tracts of mature forest habitat for breeding. Recent work suggests that at least some may shift their habitat use to early-successional areas after nesting but before migration. I used constant-effort mist netting in regenerating clearcuts (4-8 years postcut) and dense mature-forest understories to assess (1) whether most bird species of mature forests show habitat shifts after breeding; and (2), on the basis of several measures of condition, whether birds using early-successional habitats garnered any benefits or penalties, compared with those that remained in forests. I captured 3,845 individual birds of 46 species at four pairs of sites in mature Allegheny hardwood forests in northwestern Pennsylvania during the postbreeding periods of 2005-2008. Most, but not all, forest birds were captured at higher rates in cuts than in forests, and that pattern persisted through the postbreeding season. Using an information-theoretic approach, I found strong support for a species habitat interactive effect on both molt progression and body condition as measured by residuals from species-specific regression of mass on wing chord. Some, but not all, forest birds appeared to be in better condition when captured in cuts than when in forests. I found no support for a habitat effect on presence of fat or ectoparasites. My results reveal that habitat choice in the postbreeding season is correlated with physiological condition for a subset of forest birds, which suggests that the maintenance of such early-successional habitats in mature forest may benefit these species. Received 15 November 2012, accepted 2 May 2103. C1 US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. RP Stoleson, SH (reprint author), US Forest Serv, USDA, No Res Stn, POB 267, Irvine, PA 16329 USA. EM sstoleson@fs.fed.us NR 70 TC 1 Z9 1 U1 2 U2 24 PU AMER ORNITHOLOGISTS UNION PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0004-8038 EI 1938-4254 J9 AUK JI AUK PD JUL PY 2013 VL 130 IS 3 BP 417 EP 428 DI 10.1525/auk.2013.12214 PG 12 WC Ornithology SC Zoology GA 200RB UT WOS:000323085500003 ER PT J AU Lin, H Islam, MS Morano, L Groves, R Bextine, B Civerolo, E Walker, MA AF Lin, H. Islam, M. S. Morano, L. Groves, R. Bextine, B. Civerolo, E. Walker, M. A. TI GENETIC VARIATION OF XYLELLA FASTIDIOSA ASSOCIATED WITH GRAPEVINES IN TWO MAJOR VITICULTURAL REGIONS IN THE UNITED STATES: CALIFORNIA AND TEXAS SO JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Pierce's disease (PD); Xylella fastidiosa grape strains; simple sequence repeat (SSR); genetic diversity; genetic structure ID OLEANDER LEAF SCORCH; PIERCES-DISEASE; POPULATION-STRUCTURE; SAO-PAULO; STRAINS; DIVERSITY; CITRUS; BRAZIL; BACTERIA; PCR AB Xylella fastidiosa (Xf) causes diseases in many agricultural, horticultural, and landscape crops, including Pierce's disease (PD) of grapevines. PD has been a serious problem in scattered table, wine and raisin grape growing regions throughout California, as well as in Texas for more than 100 years. In this study, we evaluated the genetic variation in diversity and structure of Xf associated with PD of grapevines in California and Texas using simple sequence repeat (SSR) markers. The analysis revealed a high level of genetic diversity across the populations of Xf isolated from the grapevines from different viticulture locations or counties in California and Texas. Overall genetic diversity of Xf was detected comparatively high in Texas (0.835) than that in California (0.662). Hierarchical analysis of molecular variance (AMOVA) showed a significant genetic structure of Xf population between California and Texas. UPGMA clustering analysis and the Bayesian clustering algorithm using STRUCTURE consistently placed California and Texas Xf populations into two major genetic groups with no clear population structure within each state. C1 [Lin, H.; Islam, M. S.; Civerolo, E.] USDA ARS, Crop Dis Pests & Genet Res Unit, Parlier, CA 93648 USA. [Morano, L.] Univ Houston Downtown, Dept Nat Sci, Houston, TX 77002 USA. [Groves, R.] Univ Wisconsin, Dept Entomol, Madison, WI 53706 USA. [Bextine, B.] Univ Texas Tyler, Dept Biol, Tyler, TX 75799 USA. [Islam, M. S.; Walker, M. A.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. RP Lin, H (reprint author), USDA ARS, Crop Dis Pests & Genet Res Unit, Parlier, CA 93648 USA. EM Hong.Lin@ars.usda.gov FU California Department of Food and Agriculture Pierce's Disease and Glassy-winged Sharpshooter Board; Texas Pierce's Disease Research and Education Program FX We gratefully acknowledge the financial support by the California Department of Food and Agriculture Pierce's Disease and Glassy-winged Sharpshooter Board, Texas Pierce's Disease Research and Education Program. We appreciate the technical support provided by Parminder Sahota, USDA-ARS, Parlier, CA and Dr. Mark Black, Texas Agrilife Extension Service, Uvalde, TX. We also acknowledge the work of Dennis Garcia from the University of Houston-Downtown for providing DNA and conducting PCR for the Texas samples. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 51 TC 1 Z9 1 U1 2 U2 19 PU EDIZIONI ETS PI PISA PA PIAZZA CARRARA 16-19, 56126 PISA, ITALY SN 1125-4653 J9 J PLANT PATHOL JI J. Plant Pathol. PD JUL PY 2013 VL 95 IS 2 BP 329 EP 337 PG 9 WC Plant Sciences SC Plant Sciences GA 193QF UT WOS:000322575200011 ER PT J AU Shen, W Halbert, SE Dickstein, E Manjunath, KL Shimwela, MM van Bruggen, AHC AF Shen, W. Halbert, S. E. Dickstein, E. Manjunath, K. L. Shimwela, M. M. van Bruggen, A. H. C. TI OCCURRENCE AND IN-GROVE DISTRIBUTION OF CITRUS HUANGLONGBING IN NORTH CENTRAL FLORIDA SO JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Ca. Liberibacter asiaticus; edge effect; geostatistics; HLB; monomolecular model ID CANDIDATUS LIBERIBACTER ASIATICUS; HEMIPTERA PSYLLIDAE; FIELD POPULATIONS; DIAPHORINA-CITRI; GREENING DISEASE; TRANSMISSION; PCR; MANAGEMENT AB Citrus huanglongbing (HLB) has affected the citrus industry severely since it was first observed in south Florida in 2005. No published information is available on occurrence and in-grove distribution of HLB and its psyllid vector Diaphorina citri in north central Florida. D. citri and citrus leaf samples were collected from one organic and two conventional groves in Citra, FL, and from individual trees on the University of Florida (UF) campus in Gainesville to be tested for Candidatus Liberibacter asiaticus (Las) using qPCR. Las-positive D. citri samples were detected in Gainesville in 2007, and Las-positive trees were first found on the UF campus in October 2011. Las positive D. citri samples were obtained from symptomless trees in the organic grove in 2006 and 2007, and Las was first detected in leaf samples from asymptomatic orange trees in spring 2012. Trees in the conventional groves were Las-negative. HLB symptoms were first found in autumn 2012 in the organic grove and to a lesser extent in one conventional grove. HLB incidence was high along the northeastern edge of the groves. Semivariograms for within-row and across-row HLB spread were constructed using Las Ct values and tree distances. Monomolecular models were fit to the semivariance data. The range (=zone of influence) of HLB-infected trees was greater within rows (20 m) than across rows (13 m), suggesting that D. citri transmits HLB more readily within than across rows. Infection of conventional trees was probably delayed due to surrounding windbreaks. There was no relation between nutrient contents and HLB development. C1 [Shen, W.; Dickstein, E.; Shimwela, M. M.; van Bruggen, A. H. C.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Shen, W.; Dickstein, E.; Shimwela, M. M.; van Bruggen, A. H. C.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32611 USA. [Shen, W.] Nanjing Normal Univ, Dept Environm Sci & Engn, Nanjing 210023, Jiangsu, Peoples R China. [Halbert, S. E.] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32614 USA. [Manjunath, K. L.] ARS, USDA, Riverside, CA 92507 USA. RP van Bruggen, AHC (reprint author), Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. EM ahcvanbruggen@ufl.edu FU Emerging Pathogens Institute; Smallwood Foundation FX The authors thank the Director of the Emerging Pathogens Institute, Dr. J. Glenn Morris Jr., M.D., M.P.H.&T.M., for providing funding for a postdoctoral salary for Dr. Weishou Shen, and the trustees of the Smallwood Foundation for providing additional funding for huanglongbing research at the Emerging Pathogens Institute. The authors also thank Dr. Ganyu Gu, Debra J. Jones, Jim Boyer, and Buck Nelson for their support during this study. NR 32 TC 10 Z9 10 U1 0 U2 24 PU EDIZIONI ETS PI PISA PA PIAZZA CARRARA 16-19, 56126 PISA, ITALY SN 1125-4653 J9 J PLANT PATHOL JI J. Plant Pathol. PD JUL PY 2013 VL 95 IS 2 BP 361 EP 371 PG 11 WC Plant Sciences SC Plant Sciences GA 193QF UT WOS:000322575200014 ER PT J AU Jomantiene, R Staniulis, J Davis, RE AF Jomantiene, R. Staniulis, J. Davis, R. E. TI PHYTOPLASMA INFECTION IN PRIZED LINDEN TILIA PLATYPHYLLOS var. LACINIATA: IS 'LACINIATA' A VALID BOTANICAL VARIETY? SO JOURNAL OF PLANT PATHOLOGY LA English DT Article DE yellows disease; PCR; 16S rDNA; taxon; plant taxonomy ID 16S RIBOSOMAL-RNA; GENE-SEQUENCES; ASTER YELLOWS; CLASSIFICATION; MOLLICUTES; LINEAGES AB Linden (Tilia platyphyllos var. laciniata) trees growing in Kaunas Botanical Garden and in the parks of Verkiu (Vilnius) and Belvederis estates in Lithuania exhibit laciniated, deformed leaves and yellowing of the veins. These trees had been planted in the parks, for the beauty of their "cut-out-elongated leaves", as a new variety of linden. However, use of the polymerase chain reaction (PCR), primed by phytoplasma universal primer pairs to amplify 16S rRNA gene sequences, followed by restriction fragment length polymorphism (RFLP) analysis, revealed that these linden trees were infected by phytoplasma strains belonging to subgroup 16SrI-B and to a new subgroup designated 16SrI-V, in the 'Candidatus Phytoplasma asteris' species of wall-less bacteria. The strains were further characterized by ribosomal protein gene sequence analysis. The experimental results have rekindled discussion of the possibility that a prized cultivar displaying unusual morphotype might be an invalid taxon. Is linden with laciniate-leaved branches a valid taxon (botanical variety), or simply a plant that has abnormal leaf morphotype caused by infection with a tiny wall-less bacterium? C1 [Jomantiene, R.; Staniulis, J.] Nat Res Ctr, LT-08412 Vilnius, Lithuania. [Davis, R. E.] ARS, Mol Plant Pathol Lab, USDA, Beltsville, MD 20705 USA. RP Jomantiene, R (reprint author), Nat Res Ctr, LT-08412 Vilnius, Lithuania. EM rasa.jomantiene@yahoo.com FU USDA-Agricultural Research Service, USA [1275-22000-246-04S]; Research Council of Lithuania [MIP-062/2011] FX This work was supported by (SCA No. 1275-22000-246-04S, MPPL) USDA-Agricultural Research Service, USA, and Research Council of Lithuania, grant no. MIP-062/2011. We thank Algirdas Ivanauskas for providing the image for Fig. 2. NR 22 TC 1 Z9 1 U1 0 U2 2 PU EDIZIONI ETS PI PISA PA PIAZZA CARRARA 16-19, 56126 PISA, ITALY SN 1125-4653 J9 J PLANT PATHOL JI J. Plant Pathol. PD JUL PY 2013 VL 95 IS 2 BP 395 EP 399 PG 5 WC Plant Sciences SC Plant Sciences GA 193QF UT WOS:000322575200018 ER PT J AU Scarpin, R Sigaut, L Pietrasanta, L McCormick, S Zheng, BL Muschietti, J AF Scarpin, Regina Sigaut, Lorena Pietrasanta, Lia McCormick, Sheila Zheng, Binglian Muschietti, Jorge TI Cajal Bodies Are Developmentally Regulated during Pollen Development and Pollen Tube Growth in Arabidopsis thaliana SO MOLECULAR PLANT LA English DT Letter ID MALE GAMETOPHYTE DEVELOPMENT; BODY; COILIN C1 [Scarpin, Regina; Muschietti, Jorge] Inst Ingn Genet & Biol Mol INGEBI CONICET, RA-1428 Buenos Aires, DF, Argentina. [Sigaut, Lorena; Pietrasanta, Lia] Univ Buenos Aires, Ctr Microscopias Avanzadas, RA-1428 Buenos Aires, DF, Argentina. [McCormick, Sheila; Zheng, Binglian] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [McCormick, Sheila; Zheng, Binglian] Univ Calif Berkeley, Dept Plant & Microbial Biol, Albany, CA 94710 USA. [Zheng, Binglian] Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China. [Zheng, Binglian] Fudan Univ, Sch Life Sci, Inst Plant Biol, Shanghai 200433, Peoples R China. [Muschietti, Jorge] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, RA-1428 Buenos Aires, DF, Argentina. RP Zheng, BL (reprint author), USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. EM zhengbl@fudan.edu.cn; prometeo@dna.uba.ar OI Muschietti, Jorge/0000-0002-5719-4833 NR 14 TC 3 Z9 3 U1 2 U2 8 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1674-2052 J9 MOL PLANT JI Mol. Plant. PD JUL PY 2013 VL 6 IS 4 BP 1355 EP 1357 DI 10.1093/mp/sst077 PG 3 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 191KB UT WOS:000322410700026 PM 23699706 ER PT J AU Li, S Zhou, LZ Feng, QN McCormick, S Zhang, Y AF Li, Sha Zhou, Liang-Zi Feng, Qiang-Nan McCormick, Sheila Zhang, Yan TI The C-Terminal Hypervariable Domain Targets Arabidopsis ROP9 to the Invaginated Pollen Tube Plasma Membrane SO MOLECULAR PLANT LA English DT Letter ID TIP GROWTH; GTPASES; PLANTS; CELL; DYNAMICS; REQUIRES; SIGNAL; MOTIF C1 [Li, Sha; Zhou, Liang-Zi; Feng, Qiang-Nan; Zhang, Yan] Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China. [McCormick, Sheila; Zhang, Yan] USDA, Ctr Plant Gene Express, Berkeley, CA 94710 USA. [McCormick, Sheila; Zhang, Yan] Univ Calif Berkeley, Berkeley, CA 94710 USA. RP Zhang, Y (reprint author), Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China. EM yzhang@sdau.edu.cn NR 13 TC 5 Z9 5 U1 0 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1674-2052 J9 MOL PLANT JI Mol. Plant. PD JUL PY 2013 VL 6 IS 4 BP 1362 EP 1364 DI 10.1093/mp/sst098 PG 3 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA 191KB UT WOS:000322410700028 PM 23770841 ER PT J AU Nayak, NR Putnam, AA Addepalli, B Lowenson, JD Chen, TS Jankowsky, E Perry, SE Dinkins, RD Limbach, PA Clarke, SG Downie, AB AF Nayak, Nihar R. Putnam, Andrea A. Addepalli, Balasubrahmanyam Lowenson, Jonathan D. Chen, Tingsu Jankowsky, Eckhard Perry, Sharyn E. Dinkins, Randy D. Limbach, Patrick A. Clarke, Steven G. Downie, A. Bruce TI An Arabidopsis ATP-Dependent, DEAD-Box RNA Helicase Loses Activity upon IsoAsp Formation but Is Restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE SO PLANT CELL LA English DT Article ID TRANS ISOMERIZATION; CIRCULAR-DICHROISM; FEMALE GAMETOPHYTE; SEED LONGEVITY; FAMILY; GERMINATION; GENES; THALIANA; IDENTIFICATION; EMBRYOGENESIS AB Orthodox seeds are capable of withstanding severe dehydration. However, in the dehydrated state, Asn and Asp residues in proteins can convert to succinimide residues that can further react to predominantly form isomerized isoAsp residues upon rehydration (imbibition). IsoAsp residues can impair protein function and can render seeds nonviable, but PROTEIN ISOASPARTYL METHYLTRANSFERASE (PIMT) can initiate isoAsp conversion to Asp residues. The proteins necessary for translation upon imbibition in orthodox seeds may be particularly important to maintain in an active state. One such protein is the large, multidomain protein, Arabidopsis thaliana PLANT RNA HELICASE75 (PRH75), a DEAD-box helicase known to be susceptible to isoAsp residue accumulation. However, the consequences of such isomerization on PRH75 catalysis and for the plant are unknown. Here, it is demonstrated that PRH75 is necessary for successful seed development. It acquires isoAsp rapidly during heat stress, which eliminates RNA unwinding (but not rewinding) competence. The repair by PIMT is able to restore PRH75's complex biochemical activity provided isoAsp formation has not led to subsequent, destabilizing conformational alterations. For PRH75, an important enzymatic activity associated with translation would be eliminated unless rapidly repaired by PIMT prior to additional, deleterious conformational changes that would compromise seed vitality and germination. C1 [Nayak, Nihar R.; Chen, Tingsu; Downie, A. Bruce] Univ Kentucky, Dept Hort, Lexington, KY 40546 USA. [Nayak, Nihar R.; Chen, Tingsu; Perry, Sharyn E.; Downie, A. Bruce] Univ Kentucky, Seed Biol Grp, Lexington, KY 40546 USA. [Putnam, Andrea A.; Jankowsky, Eckhard] Case Western Reserve Univ, Sch Med, Ctr RNA Mol Biol, Cleveland, OH 44106 USA. [Putnam, Andrea A.; Jankowsky, Eckhard] Case Western Reserve Univ, Sch Med, Dept Biochem, Cleveland, OH 44106 USA. [Addepalli, Balasubrahmanyam; Limbach, Patrick A.] Univ Cincinnati, Dept Chem, Cincinnati, OH 45221 USA. [Lowenson, Jonathan D.; Clarke, Steven G.] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA. [Perry, Sharyn E.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Dinkins, Randy D.] Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Agr Sci Ctr North A220C, Lexington, KY 40546 USA. RP Downie, AB (reprint author), Univ Kentucky, Dept Hort, Lexington, KY 40546 USA. EM adownie@uky.edu RI Perry, Sharyn/M-9177-2013; OI Perry, Sharyn/0000-0002-3011-8240; Putnam, Andrea/0000-0001-7985-142X; Jankowsky, Eckhard/0000-0001-7677-7412; Dinkins, Randy/0000-0002-2127-273X FU COBRE Protein Analytical Core (U.S. Public Health Services) [P20GM103486]; National Science Foundation [0449649]; National Institutes of Health [GM58843, GM026020] FX We thank Lynnette Dirk for PRH75 protein purification for CD and GeneMANIA investigations. David Rodgers and Trevor Creamer kindly provided access to, and instruction in the use of, the Jasco J-810 spectropolarimeter housed in the UK Center for Structural Biology and the COBRE Protein Analytical Core (U. S. Public Health Services Grant P20GM103486). Martin Chow kindly provided instruction on the use of the same instrument and assisted with its setup and data retrieval. All three provided expert advice on the interpretation of CD spectra and thermal denaturation curves. We also thank David Martin, Venkata Krishna Doppalapudi, and Kim R. Schafermeyer for excellent technical assistance during this project. This research was conducted under the auspices of National Science Foundation Grant 0449649 (A. B. D. and S. G. C.), National Institutes of Health Grant GM58843 (P. A. L.), and National Institutes of Health Grant GM026020 (S. G. C.). Two anonymous reviewers improved the clarity of the article. NR 56 TC 7 Z9 8 U1 0 U2 9 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD JUL PY 2013 VL 25 IS 7 BP 2573 EP 2586 DI 10.1105/tpc.113.113456 PG 14 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 208BO UT WOS:000323650900017 PM 23903319 ER PT J AU Ni, WM Xu, SL Chalkley, RJ Pham, TND Guan, SH Maltby, DA Burlingame, AL Wang, ZY Quail, PH AF Ni, Weimin Xu, Shou-Ling Chalkley, Robert J. Pham, Thao Nguyen D. Guan, Shenheng Maltby, Dave A. Burlingame, Alma L. Wang, Zhi-Yong Quail, Peter H. TI Multisite Light-Induced Phosphorylation of the Transcription Factor PIF3 Is Necessary for Both Its Rapid Degradation and Concomitant Negative Feedback Modulation of Photoreceptor phyB Levels in Arabidopsis SO PLANT CELL LA English DT Article ID PHYTOCHROME-INTERACTING FACTOR; SCFCDC4 UBIQUITIN LIGASE; LOOP-HELIX PROTEIN; RED-LIGHT; SIGNAL-TRANSDUCTION; PHOTOACTIVATED PHYTOCHROMES; SEEDLING PHOTOMORPHOGENESIS; CHLOROPLAST DEVELOPMENT; MASS-SPECTROMETRY; CELL ELONGATION AB Plants constantly monitor informational light signals using sensory photoreceptors, which include the phytochrome (phy) family (phyA to phyE), and adjust their growth and development accordingly. Following light-induced nuclear translocation, photoactivated phy molecules bind to and induce rapid phosphorylation and degradation of phy-interacting basic Helix Loop Helix (bHLH) transcription factors (PIFs), such as PIF3, thereby regulating the expression of target genes. However, the mechanisms underlying the signal-relay process are still not fully understood. Here, using mass spectrometry, we identify multiple, in vivo, light-induced Ser/Thr phosphorylation sites in PIF3. Using transgenic expression of site-directed mutants of PIF3, we provide evidence that a set of these phosphorylation events acts collectively to trigger rapid degradation of the PIF3 protein in response to initial exposure of dark-grown seedlings to light. In addition, we show that phyB-induced PIF3 phosphorylation is also required for the known negative feedback modulation of phyB levels in prolonged light, potentially through codegradation of phyB and PIF3. This mutually regulatory intermolecular transaction thus provides a mechanism with the dual capacity to promote early, graded, or threshold regulation of the primary, PIF3-controlled transcriptional network in response to initial light exposure, and later, to attenuate global sensitivity to the light signal through reductions in photoreceptor levels upon prolonged exposure. C1 [Ni, Weimin; Pham, Thao Nguyen D.; Quail, Peter H.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Ni, Weimin; Pham, Thao Nguyen D.; Quail, Peter H.] USDA ARS, Ctr Plant Gene Express, Albany, CA 94710 USA. [Xu, Shou-Ling; Chalkley, Robert J.; Guan, Shenheng; Maltby, Dave A.; Burlingame, Alma L.] Univ Calif San Francisco, Dept Pharmaceut Chem, San Francisco, CA 94143 USA. [Xu, Shou-Ling; Wang, Zhi-Yong] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA. RP Quail, PH (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM quail@berkeley.edu RI Wang, Zhi-Yong/E-3810-2012 OI Wang, Zhi-Yong/0000-0003-4602-3390 FU National Institutes of Health [2R01 GM-047475, 8P41GM103481]; Department of Energy [DEFG03-87ER13742, DEFG02-08ER15973]; USDA/Agriculture Research Service [5335-21000-027-00D] FX We thank Bassem Al-Sady for critical reading of the article; Scott C. Peck, Nevan Krogan, Gerard Cagney, Bassem Al-Sady, and Kathy Li for initial help with mass spectrometry analysis; Anne Pfeiffer for the protocol and primers used for the DPI-ELISA assay; and all lab members for technical support and critical discussion. This work was supported by National Institutes of Health Grant 2R01 GM-047475, Department of Energy Grant DEFG03-87ER13742, and USDA/Agriculture Research Service Current Research Information System Grant 5335-21000-027-00D to P. H. Q.; by Department of Energy Grant DEFG02-08ER15973 to Z.-Y. W.; and by National Institutes of Health Grant 8P41GM103481 to A.L.B. NR 73 TC 31 Z9 32 U1 2 U2 101 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 J9 PLANT CELL JI Plant Cell PD JUL PY 2013 VL 25 IS 7 BP 2679 EP 2698 DI 10.1105/tpc.113.112342 PG 20 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA 208BO UT WOS:000323650900024 PM 23903316 ER PT J AU Walther, B Karl, JP Booth, SL Boyaval, P AF Walther, Barbara Karl, J. Philip Booth, Sarah L. Boyaval, Patrick TI Menaquinones, Bacteria, and the Food Supply: The Relevance of Dairy and Fermented Food Products to Vitamin K Requirements SO ADVANCES IN NUTRITION LA English DT Review ID LACTIC-ACID BACTERIA; NUTRITION EPIC-HEIDELBERG; BACILLUS-SUBTILIS NATTO; CORONARY-HEART-DISEASE; HEPATOCELLULAR-CARCINOMA; VASCULAR CALCIFICATION; DIETARY PHYLLOQUINONE; QUANTITATIVE MEASUREMENT; ANTICOAGULATION THERAPY; LABELED PHYLLOQUINONE AB Vitamin K exists in the food supply as phylloquinone, a plant-based form and as menaquinones (MKs), a collection of isoprenologues mostly originating from bacterial synthesis. Although multiple bacterial species used as starter cultures for food fermentations synthesize MK, relatively little is known about the presence and distribution of MK in the food supply and the relative contribution of MK to total dietary vitamin K intake. Dairy products may be a predominant source of dietary MK in many regions of the world, and there is recent interest in enhancing the MK content of dairy products through identification and selection of MK-producing bacteria in dairy fermentations. This interest is increased by emerging evidence that current dietary recommendations based on the classic role of vitamin K as an enzyme cofactor for coagulation proteins may not be optimal for supporting vitamin K requirements in extrahepatic tissues and that MK may have unique bioactivity beyond that as an enzyme cofactor. Observational studies have reported favorable associations between MK intake and bone and cardiovascular health. Although randomized trials have provided some evidence to support the beneficial effects of MK on bone, the evidence to date is not definitive, and randomized trials have not yet examined MK intake in relation to cardiovascular outcomes. Food production practices provide a means to enhance dietary MK availability and intake. However, parallel research is needed to optimize these production practices, develop comprehensive food MK content databases, and test hypotheses of unique beneficial physiological roles of MK beyond that achieved by phylloquinone. C1 [Walther, Barbara] Res Stn Agroscope Liebefeld Posieux ALP, Bern, Switzerland. [Karl, J. Philip; Booth, Sarah L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Boyaval, Patrick] DuPont Nutr & Hlth, Za Buxieres, Dange St Romain, France. RP Walther, B (reprint author), Res Stn Agroscope Liebefeld Posieux ALP, Bern, Switzerland. EM barbara.walther@agroscope.admin.ch FU USDA, Agricultural Research Service [58-1950-7-707, 58-1235-1-193] FX Supported by the USDA, Agricultural Research Service under Cooperative Agreement No. 58-1950-7-707, and 58-1235-1-193. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. NR 106 TC 21 Z9 24 U1 4 U2 34 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 2161-8313 J9 ADV NUTR JI Adv. Nutr. PD JUL PY 2013 VL 4 IS 4 BP 463 EP 473 DI 10.3945/an.113.003855 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 201KR UT WOS:000323141000006 PM 23858094 ER PT J AU Williams, MI Dumroese, RK AF Williams, Mary I. Dumroese, R. Kasten TI Preparing for Climate Change: Forestry and Assisted Migration SO JOURNAL OF FORESTRY LA English DT Review DE assisted migration; climate change; forest management; seed transfer; seed zones ID VAR GLAUCA POPULATIONS; WESTERN UNITED-STATES; DOUGLAS-FIR; MANAGED RELOCATION; TREE POPULATIONS; SEED TRANSFER; CONSERVING BIODIVERSITY; ECOLOGICAL ADAPTATIONS; SUITABLE HABITAT; CANADIAN FORESTS AB Although plants have moved across the landscape in response to changing climate for millennia, projections of contemporary climate change suggest that forest tree species and populations will need to migrate faster than their natural ability. Therefore, climate change adaptation strategies, such as assisted migration, have gained attention since 2007. Effective implementation of assisted migration can only occur if target transfer guidelines are developed because our current seed transfer guidelines, established to guide the movement of plant materials, lack inherent spatial and temporal dynamics associated with climate change. This limitation restrains reforestation practitioners from making decisions about assisted migration. Lack of operating procedures, uncertainties about future climate conditions, risks associated with moving plants outside their current ranges, and existing policies have hampered formal actions in forest management and conservation. We review the current thinking on assisted migration of forest tree species and provide information that could facilitate implementation. C1 [Williams, Mary I.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Moscow, ID USA. [Williams, Mary I.; Dumroese, R. Kasten] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID USA. RP Williams, MI (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Moscow, ID USA. EM miwillia@mtu.edu; kdumroese@fs.fed.us FU National Center for Reforestation, Nurseries, and Genetics Resources FX This collaborative work between Michigan Technological University, School of Forest Resources and Environmental Science and the USDA Forest Service, Rocky Mountain Research Station, Grassland, Shrubland, and Desert Ecosystems Program is accomplished through Joint Venture Agreement. 11-JV-11221632-130, partially funded by the National Center for Reforestation, Nurseries, and Genetics Resources. We thank Dr. Martin Jurgensen for his contributions to this work. We also thank Randy Johnson, Bryce Richardson, Jerry Rehfeldt, and three anonymous individuals for reviewing this manuscript. NR 122 TC 37 Z9 39 U1 27 U2 167 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0022-1201 J9 J FOREST JI J. For. PD JUL PY 2013 VL 111 IS 4 BP 287 EP 297 DI 10.5849/jof.13-016 PG 11 WC Forestry SC Forestry GA 198OU UT WOS:000322933000006 ER PT J AU Tande, DL Ralph, JL Johnson, LK Scheett, AJ Hoverson, BS Anderson, CM AF Tande, Desiree L. Ralph, Jody L. Johnson, LuAnn K. Scheett, Angela J. Hoverson, Bonita S. Anderson, Cindy M. TI First Trimester Dietary Intake, Biochemical Measures, and Subsequent Gestational Hypertension Among Nulliparous Women SO JOURNAL OF MIDWIFERY & WOMENS HEALTH LA English DT Article DE preeclampsia; pregnancy; nutrition; questionnaires ID ADVERSE PERINATAL OUTCOMES; INCOME PREGNANT-WOMEN; NUTRIENT INTAKE; NUTRITIONAL-STATUS; CALCIUM SUPPLEMENTATION; VASCULAR FUNCTION; FOOD FREQUENCY; INCREASED RISK; PREECLAMPSIA; DISORDERS AB Introduction: The purpose of this study was to evaluate the relationships between first-trimester dietary factors and biochemical measures and subsequent risk of gestational hypertension. Methods: This pilot study used a prospective design utilizing a convenience sample of nulliparous women enrolled at their first prenatal visit. A total of 57 women completed the study. Participants were divided into 2 groups for data analysis: normotensive pregnancy and gestational hypertension. Results: Nearly one-quarter of study participants (22.8%) developed gestational hypertension, of whom84.6% had significant proteinuria meeting the criteria for preeclampsia. There were no significant differences inmicronutrient ormacronutrient dietary intakes between groups. Serumiron and zinc levels were lower for the gestational hypertension group compared with the normotensive pregnancy group (P =.01). Low serum zinc levels were related to a risk of developing gestational hypertension (adjusted odds ratio, 0.930; 95% confidence interval, 0.872-0.992). Discussion: Ensuring adequate intake of zinc andmonitoring serum zinc levels in nulliparous pregnant women may help to prevent or contribute to early detection of gestational hypertension. C1 [Tande, Desiree L.; Ralph, Jody L.; Anderson, Cindy M.] Univ N Dakota, Coll Nursing & Profess Disciplines, Grand Forks, ND 58202 USA. [Johnson, LuAnn K.; Scheett, Angela J.; Hoverson, Bonita S.] Univ N Dakota, USDA, ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. RP Anderson, CM (reprint author), Univ N Dakota, Coll Nursing & Profess Disciplines, 430 Oxford St,Stop 9025, Grand Forks, ND 58202 USA. EM cindy.anderson@und.edu FU Robert Wood Johnson Foundation [64202]; Sigma Theta Tau International Small Grant Award [3781]; University of North Dakota Senate Scholarly Activity Committee New Faculty Scholar Award FX This project was supported by the following grants: the Robert Wood Johnson Foundation Nurse Faculty Scholar Award (CMA, 64202), the Sigma Theta Tau International Small Grant Award (CMA, 3781) and the University of North Dakota Senate Scholarly Activity Committee New Faculty Scholar Award (CMA). NR 53 TC 6 Z9 6 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-9523 J9 J MIDWIFERY WOM HEAL JI J. Midwifery Women Health PD JUL PY 2013 VL 58 IS 4 BP 423 EP 430 DI 10.1111/jmwh.12007 PG 8 WC Nursing SC Nursing GA 202FZ UT WOS:000323200700010 PM 23895215 ER PT J AU Lima, DDCC Albuquerque, RF Rocha, BP Barros, MEG Gardner, DR Medeiros, RMT Riet-Correa, F Mendonca, FS AF Lima, Dayane D. C. C. Albuquerque, Raquel F. Rocha, Brena P. Barros, Maria Edna G. Gardner, Dale R. Medeiros, Rosane M. T. Riet-Correa, Franklin Mendonca, Fabio S. TI Lysosomal storage disease induced by the consumption of Ipomoea verbascoidea (Convolvulaceae) in goats at northeastern Brazil SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Poisonous plants; plant poisoning; lysosomal storage disease; swainsonine; goats; Ipomoea verbascoidea; Convolvulaceae ID SERICOPHYLLA; SWAINSONINE; RUMINANTS; RIEDELII; PARAIBA; HORSES AB The aim of this paper was to reproduce the poisoning of Ipomoea verbascoidea in goats and describe the epidemiological, clinical and pathological aspects of spontaneous poisoning by this plant in Pernambuco. For this, we studied the epidemiology of the disease in seven municipalities in the semiarid region of the State. Three spontaneously poisoned goats were examined and then euthanized and necropsied (Group I). To reproduce the disease, the dried leaves of I. verbascoidea containing 0.02% swainsonine were supplied at doses of 4g/kg (0.8mg swainsonine/kg) to two groups of three animals. The goats in Group II received daily doses of the plant during 40 days and were euthanized on the 41st day of the experiment. Goats from Group III received daily doses of the plant during 55 days and were euthanized on the 120th day of the experiment. Other three goats constituted the control group (Group IV). In experimental groups, the brain lesions were evaluated by histopathology; additionally the cerebellar lesions were evaluated by morphometry, by measuring the molecular layer thickness, the number of Purkinje cells and the area of the cell bodies of these cells. The main clinical signs and microscopic lesions in goats poisoned were similar to those reported by swainsonine containing plants. In goats of GII and GIII, the first nervous signs were observed between 22th and 29th days; clinically, the disease developed by these animals was similar to the spontaneous cases. The goats of GIII did not recover from the neurologic signs. These results show that the consumption of the plant by 26-28 days after observation of the first clinical signs is enough to cause irreversible damage. By morphometric analysis, the molecular layer of the cerebellum of the goats of Group I and III were thinner than those of goats in the control group, and Purkinje neurons were atrophic. It is suggested that these changes are responsible for the neurological picture observed in goats that stop eating the plant and have sequelae of poisoning. C1 [Lima, Dayane D. C. C.; Albuquerque, Raquel F.] Univ Fed Rural Pernambuco UFRPE, Dept Vet Med, Programa Posgrad Ciencia Vet, BR-52171900 Recife, PE, Brazil. [Rocha, Brena P.] Univ Fed Rural Pernambuco, Dept Vet Med, Curso Med Vet, Recife, PE, Brazil. [Barros, Maria Edna G.] Univ Fed Rural Pernambuco, DMFA, Programa Posgrad Biociencia Anim, Recife, PE, Brazil. [Gardner, Dale R.] USDA ARS, Poisonous Plants Res Lab, Logan, UT 84341 USA. [Medeiros, Rosane M. T.; Riet-Correa, Franklin] UFCG, CSTR, Hosp Vet, BR-58700000 Patos de Minas, PB, Brazil. [Mendonca, Fabio S.] Univ Fed Rural Pernambuco, DMFA, Lab Diagnost Anim, Recife, PE, Brazil. RP Mendonca, FS (reprint author), Univ Fed Rural Pernambuco, DMFA, Lab Diagnost Anim, Rua Dom Manuel Medeiros S-N, Recife, PE, Brazil. EM mendonca@dmfa.ufrpe.br NR 17 TC 1 Z9 1 U1 0 U2 4 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X J9 PESQUISA VET BRASIL JI Pesqui. Vet. Bras. PD JUL PY 2013 VL 33 IS 7 BP 867 EP 872 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 204BH UT WOS:000323339100007 ER PT J AU Jaeger, WK Plantinga, AJ Chang, H Dello, K Grant, G Hulse, D McDonnell, JJ Lancaster, S Moradkhani, H Morzillo, AT Mote, P Nolin, A Santelmann, M Wu, J AF Jaeger, W. K. Plantinga, A. J. Chang, H. Dello, K. Grant, G. Hulse, D. McDonnell, J. J. Lancaster, S. Moradkhani, H. Morzillo, A. T. Mote, P. Nolin, A. Santelmann, M. Wu, J. TI Toward a formal definition of water scarcity in natural-human systems SO WATER RESOURCES RESEARCH LA English DT Article DE marginal value; deficit; anthropocentric; water scarcity; institutions ID RIVER-BASIN MANAGEMENT; WESTERN UNITED-STATES; RIO-GRANDE BASIN; CLIMATE-CHANGE; POLICY RESPONSES; NORTH-AMERICA; VULNERABILITY; FUTURE; SUSTAINABILITY; BIODIVERSITY AB Water scarcity may appear to be a simple concept, but it can be difficult to apply to complex natural-human systems. While aggregate scarcity indices are straightforward to compute, they do not adequately represent the spatial and temporal variations in water scarcity that arise from complex systems interactions. The uncertain effects of future climate change on water scarcity add to the need for clarity on the concept of water scarcity. Starting with a simple but robust definitionthe marginal value of a unit of water wehighlight key aspects of water scarcity and illustrate its many biophysical and socioeconomic determinants. We make four central observations. First, water scarcity varies greatly across location, time, and a multitude of uses that are valued either directly or indirectly by society. Second, water scarcity is fundamentally a normative, anthropocentric concept and, thus, can and should be distinguished from the related, purely descriptive notion of water deficit. While such an anthropocentric perspective may seem limiting, it has the potential to encompass the vast range of interests that society has in water. Third, our ability to understand and anticipate changes in water scarcity requires distinguishing between the factors that affect the value or benefits of water from those affecting the costs of transforming water in space, time and form. Finally, this robust and rigorous definition of water scarcity will facilitate better communication and understanding for both policymakers and scientists. C1 [Jaeger, W. K.; Wu, J.] Oregon State Univ, Dept Agr & Resource Econ, Corvallis, OR 97331 USA. [Plantinga, A. J.] Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. [Chang, H.] Portland State Univ, Dept Geog, Portland, OR 97207 USA. [Dello, K.; Mote, P.] Oregon State Univ, Oregon Climate Change Res Inst, Corvallis, OR 97331 USA. [Grant, G.] USDA Forest Serv, Pacific NW Res Stn, Corvallis, OR USA. [Hulse, D.] Univ Oregon, Dept Landscape Architecture, Eugene, OR 97403 USA. [McDonnell, J. J.] Univ Saskatchewan, Global Inst Water Secur, Saskatoon, SK, Canada. [Lancaster, S.; Nolin, A.] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Moradkhani, H.] Portland State Univ, Dept Civil & Environm Engn, Portland, OR 97207 USA. [Morzillo, A. T.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Santelmann, M.] Oregon State Univ, Water Resources Grad Program, Corvallis, OR 97331 USA. RP Jaeger, WK (reprint author), Oregon State Univ, Dept Agr & Resource Econ, 213 Ballard Extens Hall, Corvallis, OR 97331 USA. EM wjaeger@oregonstate.edu RI Moradkhani, Hamid/B-1571-2012 FU National Science Foundation [EAR-1039192, EAR-1038899, EAR-1038925]; U.S. National Oceanic and Atmospheric Administration [NA227B] FX This material is based upon work supported by the National Science Foundation under Grant Numbers EAR-1039192, EAR-1038899, and EAR-1038925, as well as support from the U.S. National Oceanic and Atmospheric Administration, Grant NA227B. NR 83 TC 12 Z9 13 U1 3 U2 68 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 J9 WATER RESOUR RES JI Water Resour. Res. PD JUL PY 2013 VL 49 IS 7 BP 4506 EP 4517 DI 10.1002/wrcr.20249 PG 12 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA 205CP UT WOS:000323419100043 ER PT J AU Forcella, F AF Forcella, Frank TI Soybean Seedlings Tolerate Abrasion from Air-Propelled Grit SO WEED TECHNOLOGY LA English DT Article DE Herbicide-free; nonchemical; organic; physical weed control; stale seedbed ID WEED-CONTROL AB New tools for controlling weeds would be useful for soybean production in organic systems. Air-propelled abrasive grit is one such tool that performs well for in-row weed control in corn, but crop safety in soybean is unknown. Responses to abrasion by corn-cob grit of soybean seedlings were examined at VE, VC, VU, V1, V2 (emergence, cotyledon, unifoliate, first trifoliate, and second trifoliate, respectively) and combinations of these growth stages, in both greenhouse and field settings. Seedling leaf areas and dry weights in greenhouse experiments were reduced by treatments that included abrasion at VC, with the primary effect expressed through reductions in the size of the unifoliate leaf. In the field, soybean stand also was reduced by grit applications at VC, especially if followed by a second application at VU or Vi. However, soybean yield was not reduced by grit applied at any soybean stage of growth. End-of-season weed dry weights did not differ from hand-weeded checks, and weeds did not impact soybean yields. Thus, abrasive grit for in-row weed control can be applied at least twice at VE through V2 growth stages without lowering soybean yield, but applications at VC probably should be avoided. C1 USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Forcella, F (reprint author), USDA ARS, N Cent Soil Conservat Res Lab, 803 Iowa Ave, Morris, MN 56267 USA. EM Frank.Forcella@ars.usda.gov FU NC-SARE program [LNC10-322] FX Partial funding for this project was provided by the NC-SARE program (Project LNC10-322). NR 11 TC 2 Z9 2 U1 0 U2 3 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X J9 WEED TECHNOL JI Weed Technol. PD JUL-SEP PY 2013 VL 27 IS 3 BP 631 EP 635 DI 10.1614/WT-D-12-00192.1 PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 203MH UT WOS:000323296800031 ER PT J AU Wang, F Jakes, JE Geng, DL Wang, XD AF Wang, Fei Jakes, Joseph E. Geng, Dalong Wang, Xudong TI Spontaneous Phase Transformation and Exfoliation of Rectangular Single-Crystal Zinc Hydroxy Dodecylsulfate Nanomembranes SO ACS NANO LA English DT Article DE free-standing two-dimensional nanostructure; phase transformation; nanomembranes; exfoliation; nanoindentation; screw dislocations ID LAYERED DOUBLE HYDROXIDES; OSTWALDS RULE; GRAPHENE; NANOCRYSTALS; ZNO; NANOINDENTATION; NANOCOMPOSITES; TRANSPARENT; ADSORPTION; CONVERSION AB Free-standing two-dimensional (2D) nanostructures, exemplified by graphene and semiconductor nanomembranes, exhibit exotic electrical and mechanical properties and have great potential in electronic applications where devices need to be flexible or conformal to nonplanar surfaces. Based on our previous development of a substrate-free synthesis of large-area, free-standing zinc hydroxy dodecylsulfate (ZHDS) hexagonal nanomembranes, herein, we report a spontaneous phase transformation of ZHDS nanomembranes under extended reaction time. The hexagonal ZHDS sheets transformed into rectangular single crystal nanomembranes with sizes of hundreds of micrometers. They contain long-range-ordered zinc vacancies that can be fitted into an orthorhombic superlattice. A surplus of dodecylsulfate ions and a deficit of Zn2+ diffusion near the water surface are believed to be the factors that drive the phase transformation. The phase transformation starts with the formation of zinc vacancies at the topmost layer of the hexagonal hillock, and propagates along the spiral growth path of the initial hexagonal sheets, which bears a great resemblance to the classic "periodic slip process". Mechanical property characterization of ZHDS nanomembranes by nanoindentation shows they behave much like structural polymers mechanically due to the incorporation of surfactant molecules. We also developed a one-step exfoliation and dehydration method that converts ZHDS nanomembranes to ZnO nanosheets using n-butylamine. This work provides a further understanding of the growth and stability of ZnO-based nanomembranes, as well as advisory insight for the further development on solution-based synthesis of free-standing, single-crystalline 2D nanostructures. C1 [Wang, Fei; Geng, Dalong; Wang, Xudong] Univ Wisconsin, Dept Mat Sci & Engn, Madison, WI 53706 USA. [Wang, Fei] Univ Wisconsin, Dept Chem, Madison, WI 53706 USA. [Jakes, Joseph E.] USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Wang, XD (reprint author), Univ Wisconsin, Dept Mat Sci & Engn, 1509 Univ Ave, Madison, WI 53706 USA. EM xudong@engr.wisc.edu RI Wang, Xudong/A-7067-2009; Wang, Fei/E-7918-2013 FU UW-NSF Nanoscale Science and Engineering Center (NSEC) [DMR 0425880]; DARPA [N66001-11-1-4139]; NSF CAREER [CMMI-1148919] FX We thank Dr. Jian Shi for his help on TEM characterization. This research is supported by UW-NSF Nanoscale Science and Engineering Center (NSEC) (DMR 0425880), DARPA N66001-11-1-4139, and NSF CAREER CMMI-1148919. NR 50 TC 7 Z9 7 U1 1 U2 60 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1936-0851 J9 ACS NANO JI ACS Nano PD JUL PY 2013 VL 7 IS 7 BP 6007 EP 6016 DI 10.1021/nn4017108 PG 10 WC Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Chemistry; Science & Technology - Other Topics; Materials Science GA 191MO UT WOS:000322417400042 PM 23730895 ER PT J AU Puangpraphant, S Dia, VP de Mejia, EG Garcia, G Berhow, MA Wallig, MA AF Puangpraphant, Sirima Dia, Vermont P. de Mejia, Elvira Gonzalez Garcia, Guadalupe Berhow, Mark A. Wallig, Matthew A. TI Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-B p65ser(311) signaling via IB- and GSK-3 reduced phosphorylation SO BIOFACTORS LA English DT Article DE Yerba mate; mate saponins; colon cancer; inflammation; NF-B signaling ID ILEX-PARAGUARIENSIS EXTRACTS; ABERRANT CRYPT FOCI; CELL-PROLIFERATION; ARDISIA-COMPRESSA; CAMELLIA-SINENSIS; GENE-EXPRESSION; AQUEOUS EXTRACT; CANCER CELLS; GREEN TEA; CARCINOGENESIS AB Yerba mate tea (YMT) has a chemopreventive role in a variety of inflammatory diseases. The objective was to determine the capability of YMT and mate saponins to prevent azoxymethane (AOM)-induced colonic inflammation in rats. YMT (2% dry leaves, w/v, as a source of drinking fluid) (n = 15) and mate saponins (0.01% in the diet, at a concentration present in one cup of YMT) (n = 15) were given ad libitum to rats 2 weeks prior to AOM-injection until the end of the study; while control rats (n = 15) received a basal diet and drinking water. After 8-weeks of study, total colonic mucosa was scraped (n = 3 rats/group) and the remaining colons (n =12 rats/group) were cut into three equal sections and aberrant crypt foci (ACF) were analyzed. YMT reduced ACF formation from 113 (control group) to 89 (P < 0.05). YMT and mate saponins reduced the expression of proinflammatory molecules COX-2 and iNOS with concomitant reduction in p-p65 (P < 0.05). Immunohistochemical analysis of the formalin-fixed middle colons showed that YMT and mate saponins reduced the expression of p-p65(ser311) by 45.7% and 43.1%, respectively, in comparison to the control (P < 0.05). In addition, the expression of molecules upstream of NF-B such as p-IB- and p-GSK-3(Y216) was downregulated by YMT 24.7% and 24.4%, respectively (P < 0.05). Results suggest the mechanism involved in the chemopreventive effect of YMT and mate saponin consumption in AOM induced-colonic inflammation in rats is through inhibition of NF-B. (c) 2013 BioFactors, 39(4):430-440, 2013 C1 [Puangpraphant, Sirima] Chulalongkorn Univ, Dept Food Technol, Fac Sci, Bangkok 10330, Thailand. [Dia, Vermont P.; de Mejia, Elvira Gonzalez; Garcia, Guadalupe] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL USA. [Berhow, Mark A.] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Wallig, Matthew A.] Univ Illinois, Dept Pathobiol, Urbana, IL USA. RP de Mejia, EG (reprint author), 228 ERML,MC-051,1201 W Gregory Dr, Urbana, IL 61801 USA. EM edemejia@illinois.edu FU Royal Thai Government FX The Royal Thai Government for Scholarship to author SP. NR 45 TC 6 Z9 6 U1 1 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0951-6433 EI 1872-8081 J9 BIOFACTORS JI Biofactors PD JUL PY 2013 VL 39 IS 4 BP 430 EP 440 DI 10.1002/biof.1083 PG 11 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA 199JI UT WOS:000322990400011 PM 23554136 ER PT J AU Klavon, KH Lansing, SA Mulbry, W Moss, AR Felton, G AF Klavon, Katherine H. Lansing, Stephanie A. Mulbry, Walter Moss, Andrew R. Felton, Gary TI Economic analysis of small-scale agricultural digesters in the United States SO BIOMASS & BIOENERGY LA English DT Article DE Anaerobic digestion; Small-scale; Manure; Biogas; Economic analysis ID ANAEROBIC-DIGESTION; WASTE; MANURE; FEASIBILITY; METHANE; ODOR; FARM AB Anaerobic digestion (AD) is an economically viable manure treatment option for large dairies (>500 cows) in the U.S. However, roughly 90% of U.S. dairies have less than 200 cows, making this technology economically inaccessible to the vast majority of U.S. dairies. While there have been case studies of individual small dairies with anaerobic digesters, there are no comparative studies using cost data from these systems. The objectives of this study were to (1) determine the economic viability of small-scale U.S. digesters using cost data from nine existing 100 to 250-cow dairies and seven theoretical systems and (2) reevaluate the minimum size dairy farm needed for economically feasible AD in the U.S. Cash flow analysis results showed that total capital costs, capital costs per cow, and net costs per cow generally decreased with increasing herd size in existing systems. Among existing revenue streams, use of digested solids for bedding generated the highest revenue ($100 cow(-1) year(-1)), followed by biogas use for heating and/or electrical generation ($47 to $70 cow(-1) year(-1)) and CO2 credits ($7 cow(-1) year(-1)). No system had a positive cash flow under the assumed conditions (8% discount rate, 20-year term). However, six of the 16 systems had positive cash flows when 50% cost sharing was included in the analysis. Our results suggest that, with cost sharing, economically viable AD systems are possible on 250-cow dairies. Additional revenue streams, such as tipping fees for food waste, may reduce the minimum size to 100-cow dairies. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Klavon, Katherine H.; Lansing, Stephanie A.; Moss, Andrew R.; Felton, Gary] Univ Maryland, Dept Environm Sci & Technol, Renewable Energy Res Ctr, College Pk, MD 20742 USA. [Mulbry, Walter] USDA ARS, Environm Management & Byprod Lab, Beltsville, MD 20705 USA. RP Lansing, SA (reprint author), Univ Maryland, Dept Environm Sci & Technol, Renewable Energy Res Ctr, 1445 Ag Engn Bldg, College Pk, MD 20742 USA. EM slansing@umd.edu OI Lansing, Stephanie/0000-0001-9169-3823 FU Maryland Water Resources Research Center [06HQGR0090]; University of Maryland Agriculture Experiment Stations FX This work was supported by funding from the Maryland Water Resources Research Center (Grant# 06HQGR0090) and University of Maryland Agriculture Experiment Stations. We would like to thank Jon Leith, Mike Kemp, and Brad Green at the USDA Beltsville Agricultural Research Center for their support and assistance. We also wish to thank Gary Seibel and the ENST Project Development Center, as well as student assistants Faaiz Ajaz, Scott Allen, Grant Hughes-Baldwin, Ashley Belle, Anisha Gupta, Kayoko Iwata, Caiti Jackson, PJ Klavon, Sol Lisboa Kotlik, Akua Nkrumah, Ryan Novak, Owen Williams, and Freddy Witarsa. NR 73 TC 13 Z9 13 U1 2 U2 37 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD JUL PY 2013 VL 54 BP 36 EP 45 DI 10.1016/j.biombioe.2013.03.009 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 180LD UT WOS:000321595800004 ER PT J AU Vadas, PA Digman, MF AF Vadas, Peter A. Digman, Matthew F. TI Production costs of potential corn stover harvest and storage systems SO BIOMASS & BIOENERGY LA English DT Article DE Corn stover; Harvest; Storage; Production costs; Modeling ID SINGLE-PASS; CROP RESIDUES; CONVERSION; GRAIN; WET AB Corn stover has potential as a bioenergy feedstock in North America. We simulated production costs for stover harvest (three-pass and two-pass with baling or chopping, and single-pass with baling or chopping) and on-farm storage (outdoor and indoor bales, outdoor wrapped bales, and chopped stover in bags, bunks, or piles). For three- and two-pass harvest, chopping was 33-45% more expensive than baling. For baling and chopping, two-pass harvest was 25% cheaper than three-pass. Single-pass chopping harvests were on average 42% cheaper than three-pass or two-pass chopping. Single-pass baling was cheaper (4-31%) than multi-pass baling at low rates of stover collection, but more expensive (1-39%) at high rates of collection. For bales, outdoor storage of wrapped bales was cheapest. Outdoor, unwrapped bale storage, even with 12% dry matter loss, was cheaper than indoor storage. For chopped stover, storage in bags was always cheapest, followed by piles, and then bunkers. With harvest and storage together, there were four least cost systems: single-pass, ear-snap baling with wrapped bale storage; single-pass chopping with silage bag storage; and two-pass baling with wrapped-bale storage. A second group of harvest/storage systems was 25% more expensive, including single-pass, whole-plant baling with wrapped-bale storage; two-pass chopping with silage-bag storage; and three-pass baling with wrapped-bale storage. The three-pass chop harvest with silage bag storage was most expensive. Our analysis suggests all harvest and farm storage systems have tradeoffs and several systems can be economically and logistically viable. Published by Elsevier Ltd. C1 [Vadas, Peter A.; Digman, Matthew F.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Vadas, PA (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr West, Madison, WI 53706 USA. EM peter.vadas@ars.usda.gov NR 20 TC 6 Z9 6 U1 1 U2 39 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD JUL PY 2013 VL 54 BP 133 EP 139 DI 10.1016/j.biombioe.2013.03.028 PG 7 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 180LD UT WOS:000321595800015 ER PT J AU Davis, TS Crippen, TL Hofstetter, RW Tomberlin, JK AF Davis, Thomas Seth Crippen, Tawni L. Hofstetter, Richard W. Tomberlin, Jeffery K. TI Microbial Volatile Emissions as Insect Semiochemicals SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Attraction; Microbes; Yeast; Fungi; Bacteria; Insect behavior; Signaling; Orientation; Pheromones; Volatile organic compounds; Tritrophic interaction ID MEXICAN FRUIT-FLY; ANOPHELES-GAMBIAE DIPTERA; BEETLE IPS-TYPOGRAPHUS; HUMAN SKIN MICROBIOTA; ONION MAGGOT DIPTERA; BARK BEETLES; ENTEROBACTER-AGGLOMERANS; ORGANIC-COMPOUNDS; DENDROCTONUS-PONDEROSAE; BACTERIAL VOLATILES AB We provide a synthesis of the literature describing biochemical interactions between microorganisms and insects by way of microbial volatile organic compound (MVOC) production. We evaluated the functionality and ecological context of MVOC signals, and explored important metabolic pathways involved in MVOC production. The cosmopolitan distribution of microorganisms creates a context for frequent, and frequently overlooked, insect responses to microbial emissions. There are numerous instances of MVOCs being closely associated with insect feeding behaviors, but some MVOCs are also powerful repellants. Emissions from microorganisms in situ may signal aspects of habitat suitability or potential exposure to entomopathogens. In some ecosystems, bacterial or fungal volatiles can also incite insect aggregations, or MVOCs can resemble sexual pheromones that elicit mating and oviposition behaviors from responding insects. A single microorganism or MVOC can have different effects on insect behaviors, especially across species, ontogenies, and habitats. There appears to be a multipartite basis for insect responses to MVOCs, and complex tritrophic interactions can result from the production of MVOCs. Many biochemical pathways for behaviorally active volatile production by microbial species are conserved across large taxonomic groupings of microorganisms. In addition, there is substantial functional redundancy in MVOCs: fungal tissues commonly produce polyketides and short-chain alcohols, whereas bacterial tissues tend to be more commonly associated with amines and pyrazines. We hypothesize that insect olfactory responses to emissions from microorganisms inhabiting their sensory environment are much more common than currently recognized, and that these signals represent evolutionarily reliable infochemicals. Insect chemoreception of microbial volatiles may contribute to the formation of neutral, beneficial, or even harmful symbioses and provide considerable insight into the evolution of insect behavioral responses to volatile compounds. C1 [Davis, Thomas Seth] Univ Idaho, Moscow, ID 83844 USA. [Crippen, Tawni L.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Hofstetter, Richard W.] No Arizona Univ, Coll Engn Forestry & Nat Sci, Flagstaff, AZ 86011 USA. [Tomberlin, Jeffery K.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. RP Davis, TS (reprint author), Univ Idaho, Moscow, ID 83844 USA. EM tsdavis1@gmail.com RI Marion-Poll, Frederic/D-8882-2011 OI Marion-Poll, Frederic/0000-0001-6824-0180 FU REACCH Project; U.S.D.A. NIFA Award [2011-68002-30191]; National Institute of Justice, Office of Justice Programs, U.S. Department of Justice [2010-DN-BX-K243]; U.S.D.A. Forest Service Rocky Mountain Station on U.S.D.A. [08-JV-11221633-250] FX The authors are indebted to numerous sources. Financial support was provided to T. S. D. under the REACCH Project, with funds from U.S.D.A. NIFA Award #2011-68002-30191. Financial support for T. L. C and J.K.T were provided by the National Institute of Justice, Office of Justice Programs, U.S. Department of Justice through Grant 2010-DN-BX-K243. Points of view in this document are those of the authors and do not necessarily represent the official position or policies of the U.S. Department of Justice. Mention of trade names, companies, or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement of the products by the U.S. Department of Agriculture. Financial support for R.W.H was partially provided by U.S.D.A. Forest Service Rocky Mountain Station on U.S.D.A. Grant 08-JV-11221633-250. We are also grateful to Peter Witzgall for encouraging our contribution. Finally, we thank three anonymous referees for contributing their time and effort to review and improve this manuscript. NR 208 TC 65 Z9 65 U1 16 U2 157 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2013 VL 39 IS 7 SI SI BP 840 EP 859 DI 10.1007/s10886-013-0306-z PG 20 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 200NW UT WOS:000323076800005 PM 23793954 ER PT J AU Davis, TS Landolt, PJ AF Davis, Thomas Seth Landolt, Peter J. TI A Survey of Insect Assemblages Responding to Volatiles from a Ubiquitous Fungus in an Agricultural Landscape SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Applied ecology; Aureobasidium pullulans; Community ecology; Hoverflies; Insect trapping; Microbial volatiles ID SPOTTED WING DROSOPHILA; ACETIC-ACID; DIPTERA DROSOPHILIDAE; AIRBORNE FUNGI; FLORAL SCENT; ATTRACTION; YEAST; IDENTIFICATION; SUZUKII; COMBINATIONS AB We report here a first survey of insect orientation to fungal cultures and fungal volatiles from a community ecology perspective. We tested whether volatiles from a ubiquitous yeast-like fungus (Aureobasidium pullulans) are broadly attractive to insects in an agricultural landscape. We evaluated insect attraction to fungal cultures and synthetic compounds identified in fungal headspace (2-methyl-1-butanol, 3-methyl-1-butanol, 2-phenylethanol) in a spearmint (Mentha spicata L.) plantation. Three findings emerged: (1) 1,315 insects representing seven orders and 39 species oriented to traps, but 65 % of trapped insects were Dipterans, of which 80 % were hoverflies (Diptera: Syrphidae); (2) traps baited with A. pullulans caught 481 % more insects than unbaited control traps on average, and contained more diverse (Shannon's H index) and species rich assemblages than control traps, traps baited with Penicillium expansum, or uninoculated media; and (3) insects oriented in greatest abundance to a 1:1:1 blend of A. pullulans volatiles, but mean diversity scores were highest for traps baited with only 2-phenylethanol or 2-methyl-1-butanol. Our results show that individual components of fungal headspace are not equivalent in terms of the abundance and diversity of insects that orient to them. The low abundance of insects captured with P. expansum suggests that insect assemblages do not haphazardly orient to fungal volatiles. We conclude that volatiles from a common fungal species (A. pullulans) are attractive to a variety of insect taxa in an agricultural system, and that insect orientation to fungal volatiles may be a common ecological phenomenon. C1 [Davis, Thomas Seth; Landolt, Peter J.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Davis, TS (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83844 USA. EM tsdavis1@gmail.com NR 37 TC 17 Z9 17 U1 2 U2 64 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2013 VL 39 IS 7 SI SI BP 860 EP 868 DI 10.1007/s10886-013-0278-z PG 9 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 200NW UT WOS:000323076800006 PM 23564294 ER PT J AU McCormick, SP AF McCormick, Susan P. TI Microbial Detoxification of Mycotoxins SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Review DE Mycotoxin; Fumonisin; Aflatoxin; Trichothecene; Patulin; Ochratoxin; Microbial degradation; Biotransformation ID POLYKETIDE SYNTHASE GENE; HYDROLYZED FUMONISIN B-1; AFLATOXIN B-1; TRICHOTHECENE MYCOTOXINS; SACCHAROMYCES-CEREVISIAE; T-2 TOXIN; SP-NOV.; TRICHOSPORON-MYCOTOXINIVORANS; STENOTROPHOMONAS-MALTOPHILIA; FUSARIUM-SPOROTRICHIOIDES AB Mycotoxins are fungal natural products that are toxic to vertebrate animals including humans. Microbes have been identified that enzymatically convert aflatoxin, zearalenone, ochratoxin, patulin, fumonisin, deoxynivalenol, and T-2 toxin to less toxic products. Mycotoxin-degrading fungi and bacteria have been isolated from agricultural soil, infested plant material, and animal digestive tracts. Biotransformation reactions include acetylation, glucosylation, ring cleavage, hydrolysis, deamination, and decarboxylation. Microbial mycotoxin degrading enzymes can be used as feed additives or to decontaminate agricultural commodities. Some detoxification genes have been expressed in plants to limit the pre-harvest mycotoxin production and to protect crop plants from the phytotoxic effects of mycotoxins. Toxin-deficient mutants may be useful in assessing the role of mycotoxins in the ecology of the microorganisms. C1 USDA ARS NCAUR, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. RP McCormick, SP (reprint author), USDA ARS NCAUR, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. EM Susan.McCormick@ars.usda.gov NR 136 TC 29 Z9 32 U1 10 U2 161 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2013 VL 39 IS 7 SI SI BP 907 EP 918 DI 10.1007/s10886-013-0321-0 PG 12 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 200NW UT WOS:000323076800010 PM 23846184 ER PT J AU Knight, AL Witzgall, P AF Knight, Alan L. Witzgall, Peter TI Combining Mutualistic Yeast and Pathogenic Virus - A Novel Method for Codling Moth Control SO JOURNAL OF CHEMICAL ECOLOGY LA English DT Article DE Plant-microbe-insect-interaction; Herbivory; Mutualism; Chemical communication; Semiochemicals; Apple; Granulovirus ID CYDIA-POMONELLA L.; PEAR ESTER; APPLE-TREES; LEPIDOPTERA; LARVAE; GRANULOVIRUS; TORTRICIDAE; MANAGEMENT; RESISTANCE; OLETHREUTIDAE AB The combination of a pathogenic virus and mutualistic yeasts isolated from larvae of codling moth Cydia pomonella is proposed as a novel insect control technique. Apples were treated with codling moth granulovirus (CpGV) and either one of three yeasts, Metschnikowia pulcherrima, Cryptococcus tephrensis, or Aureobasidium pullulans. The combination of yeasts with CpGV significantly increased mortality of neonate codling moth larvae, compared with CpGV alone. The three yeasts were equally efficient in enhancing the activity of CpGV. The addition of brown cane sugar to yeast further increased larval mortality and the protection of fruit against larvae. In comparison, without yeast, the addition of sugar to CpGV did not produce a significant effect. A field trial confirmed that fruit injury and larval survival were significantly reduced when apple trees were sprayed with CpGV, M. pulcherrima, and sugar. We have shown earlier that mutualistic yeasts are an essential part of codling moth larval diet. The finding that yeast also enhances larval ingestion of an insect-pathogenic virus is an opportunity for the development of a novel plant protection technique. We expect the combination of yeasts and insect pathogens to essentially contribute to future insect management. C1 [Knight, Alan L.] ARS, Yakima Agr Res Lab, USDA, Wapato, WA 98951 USA. [Witzgall, Peter] Swedish Univ Agr Sci, Chem Ecol Grp, S-23053 Alnarp, Sweden. RP Knight, AL (reprint author), ARS, Yakima Agr Res Lab, USDA, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM Alan.Knight@ARS.USDA.GOV RI Witzgall, Peter/N-6429-2013 OI Witzgall, Peter/0000-0002-4697-3380 FU Linnaeus environment "Insect Chemical Ecology, Ethology, and Evolution" IC-E3 (Formas, SLU) FX We thank several co-workers at the Agricultural Research Service, U.S.D.A. in Wapato, WA. Duane Larson and Lee Ream provided technical assistance in the laboratory and field, and Dave Horton provided statistical advice. Cletus Kurtzman, U.S.D.A. in Peoria, IL provided the molecular identifications of the yeast species. P. W. is supported by the Linnaeus environment "Insect Chemical Ecology, Ethology, and Evolution" IC-E3 (Formas, SLU). NR 56 TC 8 Z9 9 U1 2 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0098-0331 J9 J CHEM ECOL JI J. Chem. Ecol. PD JUL PY 2013 VL 39 IS 7 SI SI BP 1019 EP 1026 DI 10.1007/s10886-013-0322-z PG 8 WC Biochemistry & Molecular Biology; Ecology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA 200NW UT WOS:000323076800021 PM 23881444 ER PT J AU Cottrell, TE Horton, DL AF Cottrell, Ted E. Horton, Dan L. TI Emergence of Root-feeding Weevils (Coleoptera: Curculionidae) in Central Georgia Peach Orchards SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Naupactus; Callirhopalus; Sitona; Sphenophorus; Prunus; root-feeding ID PSEUDOCNEORHINUS-BIFASCIATUS; CERVINUS; ROELOFS AB Injury to peach, Prunus persicae (L.) Batsch, roots is common by several plant pathogen species and by larvae of the peachtree borer, Synanthedon exitiosa Say (Lepidoptera: Sesiidae). External feeding injury to peach roots was observed that was not consistent with S. exitiosa injury but was suspected to be caused by the larvae of root-feeding weevil species (Coleoptera: Curculionidae). Thus, we used conical emergence traps for 3 yr to sample in unsprayed peach orchards (within the dripline of trees and at missing tree sites) to monitor for root-feeding weevils. Fuller rose beetle, Naupactus cervinus (Boheman), whitefringed beetles, Naupactus spp., and the twobanded Japanese weevil, Callirhopalus bifasciatus (Roelofs), (Coleoptera: Curculionidae) were captured at significantly higher numbers within the dripline of the tree than at missing tree sites. Adult N. cervinus emerged from soil year around; whereas, the other 2 species had seasonal emergence. In commercial peach orchards comprised of cultivars with early, mid, and late-season fruit ripening dates, N. cervinus was more abundant than other species. The later a cultivar ripens, the higher the number of insecticide applications it receives; however, we did not detect a difference in N. cervinus emergence between the cultivars. In another sprayed orchard, N. cervinus was again more common than other curculionid species captured. Damage ratings (0 - 5; none to heavy, respectively) on roots revealed a mean rating of 2.79 +/- 0.12. Year-long emergence of N. cervinus is likely why it persisted in sprayed orchards. Modification of existing pest management programs will be needed to manage N. cervinus attacking peach roots. C1 [Cottrell, Ted E.; Horton, Dan L.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Cottrell, TE (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM ted.cottrell@ars.usda.gov NR 25 TC 0 Z9 0 U1 1 U2 8 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2013 VL 48 IS 3 BP 184 EP 194 PG 11 WC Entomology SC Entomology GA 198QF UT WOS:000322936700002 ER PT J AU Spurgeon, DW Cooper, WR AF Spurgeon, D. W. Cooper, W. R. TI Sweepnet Captures of Lygus hesperus (Hemiptera: Miridae) Adult Genders and Age-Classes in Cotton SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE western tarnished plant bug; Lygus hesperus; sampling efficiency; reproductive development ID TARNISHED PLANT BUGS; REPRODUCTIVE STATES; KNIGHT HEMIPTERA; SAMPLING METHODS; HETEROPTERA; INJURY; ABSOLUTE; NET AB Management of the western tarnished plant bug, Lygus hesperus Knight, in cotton usually relies on population estimates obtained using the sweepnet. Recent studies indicated adult L. hesperus gender and physiological age influence feeding behavior, within-plant distribution, and injury to cotton. Whether these differences in behavior also influence capture by the sweepnet is not known. We evaluated the sweepnet for sampling L. hesperus adults of known gender and age class in Pima and Acala Upland cotton. Adults of 4 classes (females, prereproductive or reproductive; males, prereproductive or reproductive) marked with fingernail polish to prevent flight were released into assigned sample rows. Captures of marked adults declined seasonally in all experiments. In Pima cotton, the sweepnet was least effective for sampling prereproductive female L. hesperus, and most effective for collecting reproductive males. Captures of reproductive females and prereproductive males were intermediate. We suggest the influence of adult gender and age class on sweepnet captures in Pima cotton corresponds to a propensity for prereproductive adults to reside within plant terminals substantially shielded by foliage. Similar differences in Upland cotton were not observed, probably because the comparatively open structure of the Upland terminal minimized the effects of gender and age class on collection efficiency. Finally, dissection of native L. hesperus indicated. an age structure dominated by reproductive maturity. Therefore, future mark-release-recapture studies of factors influencing sweepnet sampling for L. hesperus adults in cotton may maximize relevance by focusing on reproductively mature adults. C1 [Spurgeon, D. W.; Cooper, W. R.] ARS, USDA, Western Integrated Cropping Syst Res Unit, Shafter, CA 93263 USA. RP Spurgeon, DW (reprint author), ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. EM Dale.Spurgeon@ars.usda.gov RI Cooper, William/D-3205-2017 FU California State Support Committee, Cotton Incorporated, Biotic Factors Influencing Sweep Net Efficiency for Western Tarnished Plant Bug Adults [11-856CA] FX The research reported herein was supported in part by the California State Support Committee, Cotton Incorporated Project No. 11-856CA, Biotic Factors Influencing Sweep Net Efficiency for Western Tarnished Plant Bug Adults. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 22 TC 0 Z9 0 U1 0 U2 2 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2013 VL 48 IS 3 BP 195 EP 205 PG 11 WC Entomology SC Entomology GA 198QF UT WOS:000322936700003 ER PT J AU Morales-Ramos, JA Rojas, MG Shapiro-Ilan, DI Tedders, WL AF Morales-Ramos, Juan A. Rojas, M. Guadalupe Shapiro-Ilan, David I. Tedders, W. Louis TI Use of Nutrient Self-Selection as a Diet Refining Tool in Tenebrio molitor (Coleoptera: Tenebrionidae) SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE yellow mealworm; nutrition; rearing; development; fecundity; production; food conversion ID IN-VIVO PRODUCTION; ENTOMOPATHOGENIC NEMATODES; PENTATOMIDAE; PREY; INVERTEBRATES; OPTIMIZATION; HETEROPTERA; VIRULENCE; EFFICACY; PREDATOR AB A new method to refine existing dietary supplements for improving production of the yellow mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae), was tested. Self-selected ratios of 6 dietary ingredients by T molitor larvae were used to produce a dietary supplement. This supplement was compared with existing supplement formulations mixed with wheat bran at 1:4 ratio and a control consisting of wheat bran alone for food utilization efficiency, larval growth, development time, immature survival, and fecundity. Ingredients of dietary supplements included dry potato as a source of carbohydrate; dry egg white and soy protein as a source of protein and; peanut, canola, and salmon oil as a source of lipid. A supplement consisting of dry potato alone significantly improved food utilization, growth, development time, survival, and fecundity compared with the wheat bran-only control group. The addition of protein to the supplement significantly shortened development time and improved food conversion efficiency and fecundity compared with the supplement with potato alone. The addition of lipid did not provide any significant improvements. The supplement derived from self-selected ratios of the basic ingredients provided a significant increase in fecundity compared with previously developed supplements and the control. Self-selected ratios of the basic ingredients by T molitor larvae had an effect on the adult stage that resulted in significantly higher progeny production. C1 [Morales-Ramos, Juan A.; Rojas, M. Guadalupe; Shapiro-Ilan, David I.; Tedders, W. Louis] ARS, USDA, NBCL, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. RP Morales-Ramos, JA (reprint author), ARS, USDA, NBCL, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. EM juan.moralesramos@ars.usda.gov OI Morales-Ramos, Juan/0000-0002-3506-3859 FU USDA-National Institute of Food and Agriculture (NIFA) [2007-33610-18416, 2007-03695] FX The authors thank the USDA-National Institute of Food and Agriculture (NIFA) for financing a portion of this research through the Small Business Innovation Research (SBIR) program (grant No. 2007-33610-18416/proposal No. 2007-03695). We also thank Sasha Kay for her assistance in rearing and colony maintenance, and Carlos Blanco, Thomas A. Coudron, and Matthew H. Greenstone for peer reviewing the manuscript. NR 28 TC 3 Z9 3 U1 2 U2 30 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2013 VL 48 IS 3 BP 206 EP 221 PG 16 WC Entomology SC Entomology GA 198QF UT WOS:000322936700004 ER PT J AU Lohmeyer, KH Pound, JM Klavons, JA Davey, RB AF Lohmeyer, Kimberly H. Pound, J. Mathews Klavons, Jerome A. Davey, R. B. TI Liquid Chromatographic Detection of Permethrin from Filter Paper Wipes of White-tailed Deer SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE southern cattle tick; lone star tick; blacklegged tick; cattle tick; permethrin; acaricide C1 [Lohmeyer, Kimberly H.; Pound, J. Mathews; Klavons, Jerome A.; Davey, R. B.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Davey, R. B.] ARS, USDA, Cattle Fever Tick Res Lab, Moore Air Base, Edinburg, TX 78541 USA. RP Lohmeyer, KH (reprint author), ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM kim.lohmeyer@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2013 VL 48 IS 3 BP 258 EP 260 PG 3 WC Entomology SC Entomology GA 198QF UT WOS:000322936700010 ER PT J AU Cooper, WR Spurgeon, DW AF Cooper, W. Rodney Spurgeon, Dale W. TI Response by Lygus hesperus (Hemiptera: Miridae) Adults to Salivary Preconditioning of Cotton Squares SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Western tarnished plant bug; saliva; Gossypium; feeding behavior C1 [Cooper, W. Rodney; Spurgeon, Dale W.] ARS, USDA, Shatter Cotton Res Stn, Shatter, CA 93263 USA. RP Cooper, WR (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM Rodney.Cooper@ars.usda.gov RI Cooper, William/D-3205-2017 NR 0 TC 1 Z9 1 U1 0 U2 5 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD JUL PY 2013 VL 48 IS 3 BP 261 EP 264 PG 4 WC Entomology SC Entomology GA 198QF UT WOS:000322936700011 ER PT J AU Zhao, Y Niu, YG Xie, ZH Shi, HM Chen, P Yu, LL AF Zhao, Yang Niu, Yuge Xie, Zhuohong Shi, Haiming Chen, Pei Yu, Liangli (Lucy) TI Differentiating leaf and whole-plant samples of di- and tetraploid Gynostemma pentaphyllum (Thunb.) Makino using flow-injection mass spectrometric fingerprinting method SO JOURNAL OF FUNCTIONAL FOODS LA English DT Article DE Gynostemma pentaphyllum; Flow-injection mass spectrometric; fingerprinting method; Chemometrics; Diploid; Tetraploid ID PRINCIPAL COMPONENT; MSN AB In the present study, the feasibility and advantages of a flow-injection mass spectrometric (FIMS) fingerprinting method combined with chemometrics for quick differentiation and quality assessment of di- and tetraploid leaf and whole-plant Gynostemma pentaphyllum (Thunb.) Makino (Jiaogulan in Chinese) were investigated for the first time. The rapid FIMS fingerprinting method was applied to generate spectrometric fingerprints of four different types of G. pentaphyllum samples. Chemometrics, including principal component analysis (PCA) and partial least square discriminative analysis (PLS-DA), were used to analyze the fingerprints. The results showed that each sample could be effectively differentiated and classified in 2 min. Furthermore, eleven characteristic ions that played the most important roles for differentiating the four groups of G. pentaphyllum samples were screened out by loadings plot from PLS-DA. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Zhao, Yang; Niu, Yuge; Xie, Zhuohong; Yu, Liangli (Lucy)] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Niu, Yuge; Shi, Haiming; Yu, Liangli (Lucy)] Shanghai Jiao Tong Univ, Sch Agr & Biol, Inst Food & Nutraceut Sci, Key Lab Urban Agr South, Shanghai 200240, Peoples R China. [Chen, Pei] ARS, Food Composit & Methods Dev Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Shi, HM (reprint author), Shanghai Jiao Tong Univ, Sch Agr & Biol, Inst Food & Nutraceut Sci, Key Lab Urban Agr South, Shanghai 200240, Peoples R China. EM hmshi@sjtu.edu.cn; pei.chen@ars.usda.gov RI ZHAO, YANG/O-7920-2014; Xie, Zhuohong/P-3599-2014; shi, haiming/D-2806-2015 OI Xie, Zhuohong/0000-0001-8009-3523; NR 21 TC 6 Z9 8 U1 2 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1756-4646 J9 J FUNCT FOODS JI J. Funct. Food. PD JUL PY 2013 VL 5 IS 3 BP 1288 EP 1297 DI 10.1016/j.jff.2013.04.013 PG 10 WC Food Science & Technology SC Food Science & Technology GA 195GZ UT WOS:000322691600030 ER PT J AU Semeao, AA Campbell, JF Whitworth, RJ Sloderbeck, PE AF Semeao, Altair A. Campbell, James F. Whitworth, R. Jeff Sloderbeck, Phillip E. TI Movement of Tribolium castaneum within a flour mill SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Red flour beetle; Flour mill; Mark-recapture; Dispersal; Heat treatment ID STORED-PRODUCT INSECTS; COLEOPTERA-TENEBRIONIDAE; SPATIOTEMPORAL DYNAMICS; PLODIA-INTERPUNCTELLA; SPATIAL-DISTRIBUTION; TRAP CATCHES; FEED MILL; FLIGHT; FOOD; POPULATIONS AB The colonization of food processing plants by stored-product pests and their distribution within a facility depend, in part, on their dispersal ability. In this case study, we relied on self-mark recapture to evaluate the ability of Tribolium castaneum, the red flour beetle, to move among floors within a flour mill and the effects of a heat treatment on insect activity. Marking stations with pheromone and fluorescent powder were placed on each of five floors in the mill, and two techniques were used to recover marked individuals (trapping and direct collection of individuals from the floor). Considering both recovery techniques, T. castaneum was able to move among floors, but the majority of individuals remained on the same floor where they were marked (86%). Most individuals captured on a different floor were captured on a floor below the one they were marked (70%) and adjacent to it (87%). There was a spike in the number of beetles captured during heat treatment, but not an increase in movement of marked beetles between floors. These results suggest that the rate of heating was sufficient to prevent beetles time to move to cooler floors to escape heat. T. castaneum movement among floors needs to be taken into account when identifying sources of infestation and targeting pest management. Published by Elsevier Ltd. C1 [Semeao, Altair A.; Whitworth, R. Jeff] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Campbell, James F.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Sloderbeck, Phillip E.] Kansas State Univ, Southwest Res & Extens Ctr, Garden City, KS 67846 USA. RP Campbell, JF (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov FU USDA RAMP grant [2007-51101-18407]; USDA Methyl Bromide Alternative grant [2011-51102-31125] FX We thank the personnel at the Hall Ross Flour Mill, Department of Grain Science and Industry, Kansas State University and Dr. Bh. Subramanyam for facilitating study and Dr. R. N. C. Guedes for providing comments on an earlier version of the manuscript. This study was supported in part by USDA RAMP (Agreement No. 2007-51101-18407) and USDA Methyl Bromide Alternative (Agreement No. 2011-51102-31125) grants. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or Kansas State University. USDA is an equal opportunity provider and employer. Contribution number 13-182-J from the Kansas Agricultural Experiment Station. NR 34 TC 7 Z9 7 U1 0 U2 22 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X J9 J STORED PROD RES JI J. Stored Prod. Res. PD JUL PY 2013 VL 54 BP 17 EP 22 DI 10.1016/j.jspr.2013.03.004 PG 6 WC Entomology SC Entomology GA 199UM UT WOS:000323021000004 ER PT J AU Uhrig, SR Nol, P McCollum, M Salman, M Rhyan, JC AF Uhrig, Samantha R. Nol, Pauline McCollum, Matt Salman, Mo Rhyan, Jack C. TI EVALUATION OF TRANSMISSION OF BRUCELLA ABORTUS STRAIN 19 IN BISON BY INTRAVAGINAL, INTRAUTERINE, AND INTRACONJUNCTIVAL INOCULATION SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Bison; Brucella abortus; brucellosis; strain 19; venereal transmission ID YELLOWSTONE-NATIONAL-PARK; PATHOGENESIS; SEROLOGY AB Bovine brucellosis, caused by the bacterium Brucella abortus, is endemic in bison (Bison bison) and elk (Germs elaphus nelsoni) populations in the area of Yellowstone. National Park, USA. Two strategies have been proposed to reduce the risk of transmission, of disease in bison remote vaccination with the vaccine RB51, and the use of immunocontraception of bison to decrease shedding of organisms from infected females. The frequent occurrence of venereal transmission in bison would complicate either of these strategies, requiring vaccination of males as well as females, and rendering immunocontraception less effective in reducing transmission of B. abortus. To address the question of venereal transmission, we inoculated each of 18 bison cows with 4.5 X 10(8) colony-forming units of B. abortus strain 19, as a surrogate of field strain, by three routes: intraconjunctival (IC), intravaginal (VI), and intracervical/intrauterine (AI). Bison semen was mixed with strain 19 inoculum for the latter route. Bison were monitored by serology and culture for 12 wk, at which time they were euthanized and specimens collected for culture. All IC-inoculated animals seroconverted on multiple tests and one was culture positive at 12 wk postexposure. Seven of eight VI bison developed suspect or positive serologic tests and four were positive at one or more time points. Weak transient serologic responses (suspect) were seen in four of five AI bison. Results showed that IC inoculation with strain 19 was a suitable surrogate for field strain to demonstrate exposure to the B. abortus. The seroconversion of four of eight VI bison indicated exposure of the immune system to the agent and the need for further studies on venereal transmission in bison. C1 [Uhrig, Samantha R.] Colorado State Univ, Dept Clin Sci, Ft Collins, CO 80523 USA. [Nol, Pauline; McCollum, Matt; Rhyan, Jack C.] USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Vet Serv, Ft Collins, CO 80521 USA. [Salman, Mo] Colorado State Univ, Anim Populat Hlth Inst, Ft Collins, CO 80523 USA. RP Rhyan, JC (reprint author), USDA, Natl Wildlife Res Ctr, Anim & Plant Hlth Inspect Serv, Vet Serv, Ft Collins, CO 80521 USA. EM jack.c.rhyan@aphis.usda.gov FU USDA; Colorado State University, Animal Population Health Institute FX Cooperative funding for this project was provided by the USDA and Colorado State University, Animal Population Health Institute. Sincere thanks to Karl Held and Roger Thompson for animal care and handling, the NVSL, and the Montana Veterinary Diagnostic Laboratory. This project was approved by the Colorado State University Institutional Animal Care and Use Committee. NR 21 TC 1 Z9 1 U1 3 U2 17 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2013 VL 49 IS 3 BP 522 EP 526 DI 10.7589/2012-03-071 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 183FA UT WOS:000321798500006 PM 23778600 ER PT J AU Dirsmith, K VanDalen, K Fry, T Charles, B VerCauteren, K Duncan, C AF Dirsmith, Katherine VanDalen, Kaci Fry, Tricia Charles, Brad VerCauteren, Kurt Duncan, Colleen TI Leptospirosis in Fox Squirrels (Sciurus niger) of Larimer County, Colorado, USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Colorado; Leptospira interrogans; leptospirosis; squirrel; wildlife ID UNITED-STATES AB Leptospirosis is a zoonotic disease caused by the bacterium Leptospira interrogans. The organism is typically maintained within a geographic region by colonizing renal tubules of carrier animals and shed into the environment in urine. We assessed whether L. interrogans was present in fox squirrels (Sciurus niger) in Larimer County, Colorado, USA, and whether it is associated with disease. Twenty-two squirrels were trapped from 29 November 2011 to 15 December 2011 for use in an unrelated study. The squirrels were individually housed for 33-65 days and euthanized; no clinical disease was observed. On gross examination, significant renal lesions were observed in 6 of 22 animals (27%). Histologically, affected animals had severe neutrophilic tubulitis with interstitial nephritis Immunohistochemistry was conducted on the kidneys of all animals and 10 of 22 (45%) were positive for L. interrogans, with varying severity of infection. The same 10 squirrels were serologically positive for antibodies specific to L. interrogans. These results suggest that L. interrogans is present in fox squirrels in Larimer County, Colorado, USA, and may be associated with varying degrees of renal disease. Further investigation into the role of wildlife in the ecology of leptospirosis within the region is warranted. C1 [Dirsmith, Katherine; Charles, Brad; Duncan, Colleen] Colorado State Univ, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. [VanDalen, Kaci; Fry, Tricia; VerCauteren, Kurt] Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Duncan, C (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, 1601 Campus Delivery, Ft Collins, CO 80523 USA. EM colleen.duncan@colostate.edu NR 10 TC 1 Z9 1 U1 1 U2 18 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2013 VL 49 IS 3 BP 641 EP 645 DI 10.7589/2012-10-265 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 183FA UT WOS:000321798500020 PM 23778614 ER PT J AU Pilon, JL Rhyan, JC Wolfe, LL Davis, TR McCollum, MP O'Rourke, KI Spraker, TR VerCauteren, KC Miller, MW Gidlewski, T Nichols, TA Miller, LA Nol, P AF Pilon, John L. Rhyan, Jack C. Wolfe, Lisa L. Davis, Tracy R. McCollum, Matt P. O'Rourke, Katherine I. Spraker, Terry R. VerCauteren, Kurt C. Miller, Michael W. Gidlewski, Thomas Nichols, Tracy A. Miller, Lowell A. Nol, Pauline TI Immunization with a Synthetic Peptide Vaccine Fails to Protect Mule Deer (Odocoileus hemionus) from Chronic Wasting Disease SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Cervid; chronic wasting disease; mule deer; prion; vaccine ID CERVUS-ELAPHUS NELSONI; WHITE-TAILED DEER; PRPCWD; PATTERNS AB Chronic wasting disease (CWD) adversely affects both wild and captive cervid populations. A vaccine to prevent CWD would be a highly desirable tool to aid in disease management. To this end, we tested in mule deer a combination of CWD vaccines consisting of cervid prion peptide sequences 168-VDQYNNQNTFVHDC-182 and 145-NDYEDRYYRENMYRYPNQ-164 that had previously been shown to delay onset of clinical disease and increase survival in a mouse-adapted scrapie model. Thirteen captive mule deer (Odocoileus hemionus) were divided into vaccine (n=7) and control groups (n=6), and given prime and boost vaccinations intramuscularly 5 wk apart. Eight weeks postprime (3 wk postboost), all animals were challenged via natural exposure to an environment contaminated with infective CWD prions. Deer were monitored intermittently for prion infection by rectal and tonsil biopsies beginning 275 days postchallenge. All vaccinates responded to both peptide conjugates present in the combination vaccine as measured by enzyme-linked immunosorbent assay. However, all deer eventually became infected regardless of vaccine status. C1 [Pilon, John L.; VerCauteren, Kurt C.; Gidlewski, Thomas; Nichols, Tracy A.; Miller, Lowell A.] APHIS, USDA, WS, NWRC, Ft Collins, CO 80521 USA. [Rhyan, Jack C.; McCollum, Matt P.; Nol, Pauline] APHIS, USDA, Vet Serv, NWRC, Ft Collins, CO 80521 USA. [Wolfe, Lisa L.; Davis, Tracy R.; Miller, Michael W.] Colorado Div Pk & Wildlife, Ft Collins, CO 80521 USA. [O'Rourke, Katherine I.] Washington State Univ, ARS, USDA, Pullman, WA 99164 USA. [Spraker, Terry R.] Colorado State Univ, Dept Pathol & Microbiol, Ft Collins, CO 80523 USA. [O'Rourke, Katherine I.] Washington State Univ, Coll Vet Med, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. RP Nol, P (reprint author), APHIS, USDA, Vet Serv, NWRC, Ft Collins, CO 80521 USA. EM pauline.nol@aphis.usda.gov FU USDA/APHIS Science Fellow program; Colorado Division of Parks and Wildlife FX We acknowledge the following individuals for their valuable time and assistance with this work: Tracy Sirochman, Michael Sirochman, Karl Held, Mike Lavelle, Justin Fischer, Seth Nienhauser, Richanne Lomkin, and Tara Rigg, and other volunteers and seasonal assistants. This work was supported by the USDA/APHIS Science Fellow program and the Colorado Division of Parks and Wildlife. NR 15 TC 5 Z9 5 U1 3 U2 18 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2013 VL 49 IS 3 BP 694 EP 698 DI 10.7589/2012-07-200 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 183FA UT WOS:000321798500030 PM 23778624 ER PT J AU Pedersen, KI Bevins, SN Baroch, JA Cumbee, JC Chandler, SC Woodruff, BS Bigelow, TT DeLiberto, TJ AF Pedersen, Kern I. Bevins, Sarah N. Baroch, John A. Cumbee, James C., Jr. Chandler, Shannon C. Woodruff, Billy S. Bigelow, Troy T. DeLiberto, Thomas J. TI Pseudorabies in Feral Swine in the United States, 2009-2012 SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Aujeszky's disease; feral swine; gB ELISA; pseudorabies; surveillance ID AUJESZKYS-DISEASE VIRUS; BLACK BEAR; WILD BOAR; TRANSMISSION; FLORIDA; POPULATIONS; PREVALENCE; ANTIBODIES AB Although pseudorabies virus can affect a wide range of mammalian and avian hosts, swine are the only natural hosts of the virus. The US commercial swine industry obtained pseudorabies-free status in 2004, which was important because of the economic value of domestic swine production; however, feral swine remain competent hosts and represent a constant threat for reintroducing the virus into the commercial industry. To better assess feral swine infection status, we collected 8,498 serum samples from feral swine across the United States between 1 October 2009 and 30 September 2012. Of these, 18% were antibody positive in 25 of 35 states where samples were collected, indicating that transmission risk is widespread. C1 [Pedersen, Kern I.; Bevins, Sarah N.; Baroch, John A.; DeLiberto, Thomas J.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Cumbee, James C., Jr.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Mississippi State, MS 39762 USA. [Chandler, Shannon C.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Sacramento, CA 95825 USA. [Woodruff, Billy S.] Anim & Plant Hlth Inspect Serv, USDA, Port Allen, LA 70767 USA. [Bigelow, Troy T.] Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Des Moines, IA 50309 USA. RP Pedersen, KI (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Kerri.Pedersen@aphis.usda.gov NR 18 TC 6 Z9 9 U1 1 U2 22 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2013 VL 49 IS 3 BP 709 EP 713 DI 10.7589/2012-12-314 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 183FA UT WOS:000321798500033 PM 23778627 ER PT J AU Frey, RK Clarke, PR McCollum, MP Nol, P Johnson, KR Thompson, BD Ramsey, JM Anderson, NJ Rhyan, JC AF Frey, Rebecca K. Clarke, P. Ryan McCollum, Matt P. Nol, Pauline Johnson, Kammy R. Thompson, Brent D. Ramsey, Jennifer M. Anderson, Neil J. Rhyan, Jack C. TI Evaluation of Bison (Bison bison) Semen from Yellowstone National Park, Montana, USA, Bulls for Brucella abortus Shedding SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Bison; brucellosis; culture; ejaculation; semen; serology ID INFECTION AB To determine if bison (Bison bison) bulls from Yellowstone National Park (YNP), Montana, USA, shed an infective dose of Brucella abortus in semen, 50 YNP bulls were captured on public lands in Montana during the winter and early spring (April-May) of 2010 and 2011. The bulls were immobilized, and blood and semen samples were collected for serology and Brucella culture. Thirty-five bulls (70%) were antibody-positive, and B. abortus was cultured from semen in three (9%) of the 35 antibody-positive or suspect bulls, though not at concentrations considered an infective dose. Eight bulls (six antibody-positive, two negative) had palpable lesions of the testes, epididymides, or seminal vesicles consistent with B. abortus infection. Breeding soundness exams and semen analysis suggested that antibody-positive bulls were more likely to have nonviable ejaculate (8/35; 23%) than bulls without detectable antibody (2/15; 13%). C1 [Frey, Rebecca K.; Clarke, P. Ryan] APHIS, USDA, Vet Serv VS Western Reg, Ft Collins, CO 80526 USA. [McCollum, Matt P.; Nol, Pauline; Rhyan, Jack C.] APHIS, USDA, VS, Ft Collins, CO 80521 USA. [Johnson, Kammy R.; Thompson, Brent D.] APHIS, USDA, VS, Helena, MT 59601 USA. MFWP, Bozeman, MT 59718 USA. RP Frey, RK (reprint author), APHIS, USDA, Vet Serv VS Western Reg, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM rebecca.k.frey@aphis.usda.gov NR 16 TC 0 Z9 0 U1 5 U2 11 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD JUL PY 2013 VL 49 IS 3 BP 714 EP 717 DI 10.7589/2012-04-118 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA 183FA UT WOS:000321798500034 PM 23778628 ER PT J AU Hansen, S Clay, SA Clay, DE Carlson, CG Reicks, G Jarachi, Y Horvath, D AF Hansen, Stephanie Clay, Sharon A. Clay, David E. Carlson, C. Gregg Reicks, Graig Jarachi, Youssef Horvath, David TI Landscape Features Impact on Soil Available Water, Corn Biomass, and Gene Expression during the Late Vegetative Stage SO PLANT GENOME LA English DT Article ID CIRCADIAN CLOCK; DROUGHT STRESS; PLANT; NITROGEN; MAIZE; RESPONSES; YIELD; PHOTOSYNTHESIS; MICROARRAYS; METABOLISM AB Crop yields at summit positions of rolling landscapes often are lower than backslope yields. The differences in plant response may be the result of many different factors. We examined corn (Zea mays L.) plant productivity, gene expression, soil water, and nutrient availability in two landscape positions located in historically high (backslope) and moderate (summit and shoulder) yielding zones to gain insight into plant response differences. Growth characteristics, gene expression, and soil parameters (water and N and P content) were determined at the V12 growth stage of corn. At tassel, plant biomass, N content, C-13 isotope discrimination (Delta), and soil water was measured. Soil water was 35% lower in the summit and shoulder compared with the lower backslope plots. Plants at the summit had 16% less leaf area, biomass, and N and P uptake at V12 and 30% less biomass at tassel compared with plants from the lower backslope. Transcriptome analysis at V12 indicated that summit and shoulder-grown plants had 496 downregulated and 341 upregulated genes compared with backslope-grown plants. Gene set and subnetwork enrichment analyses indicated alterations in growth and circadian response and lowered nutrient uptake, wound recovery, pest resistance, and photosynthetic capacity in summit and shoulder-grown plants. Reducing plant populations, to lessen demands on available soil water, and applying pesticides, to limit biotic stress, may ameliorate negative water stress responses. C1 [Hansen, Stephanie; Clay, Sharon A.; Clay, David E.; Carlson, C. Gregg; Reicks, Graig; Jarachi, Youssef] S Dakota State Univ, Dep Plant Sci, Brookings, SD 57007 USA. [Horvath, David] ARS, USDA, BRL, Fargo, ND 58102 USA. RP Horvath, D (reprint author), ARS, USDA, BRL, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM david.horvath@ars.usda.gov OI Horvath, David/0000-0002-8458-7691 FU South Dakota Agricultural Experiment Station; South Dakota Corn Utilization Council; South Dakota Soybean Research and Promotion Council; USDA-CSREES-seed technology grant [2008-34556-19350]; South Dakota initiative; USDA-CSREES-NRI [2009-35320-05040] FX Support for this project was provided by the South Dakota Agricultural Experiment Station, South Dakota Corn Utilization Council, South Dakota Soybean Research and Promotion Council, a USDA-CSREES-seed technology grant (2008-34556-19350), the South Dakota 2010 initiative, and a USDA-CSREES-NRI grant (2009-35320-05040). NR 53 TC 4 Z9 4 U1 2 U2 13 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2013 VL 6 IS 2 DI 10.3835/plantgenome2012.11.0029 PG 9 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA 197YC UT WOS:000322887700007 ER PT J AU Ziegler, G Terauchi, A Becker, A Armstrong, P Hudson, K Baxter, I AF Ziegler, Greg Terauchi, Aimee Becker, Anthony Armstrong, Paul Hudson, Karen Baxter, Ivan TI Ionomic Screening of Field-Grown Soybean Identifies Mutants with Altered Seed Elemental Composition SO PLANT GENOME LA English DT Article ID ARABIDOPSIS-THALIANA; PACKAGE AB Soybean [Glycine max (L.) Merr.] seeds contain high levels of mineral nutrients essential for human and animal nutrition. High throughput elemental profiling (ionomics) has identified mutants in model plant species grown in controlled environments. Here, we describe a method for identifying potential soybean ionomics mutants grown in a field setting and apply it to 975 N-nitroso-N-methylurea (NMU) mutagenized lines. After performing a spatial correction, we identified mutants using either visual scoring of standard score (z-score) plots or computational ranking of putative mutants followed by visual confirmation. Although there was a large degree of overlap between the methods, each method identified unique lines. The visual scoring approach identified 22 out of 427 (5%) potential mutants, 70% (16 out of 22) of which were confirmed when seeds from the same parent plant were regrown in the field. We also performed simulations to determine an optimal strategy for screening large populations. All data from the screen is available at the Ionomics Hub File Transfer Page (http://www.ionomicshub.org/home/PiiMS/dataexchange). C1 [Ziegler, Greg; Becker, Anthony] Donald Danforth Plant Sci Ctr, St Louis, MO USA. [Terauchi, Aimee; Baxter, Ivan] ARS, USDA, Plant Genet Res Unit, St Louis, MO USA. [Armstrong, Paul] ARS, USDA, GGAHR, Manhattan, KS USA. [Hudson, Karen] ARS, USDA, W Lafayette, IN USA. RP Baxter, I (reprint author), ARS, USDA, Plant Genet Res Unit, St Louis, MO USA. EM Ivan.Baxter@ars.usda.gov RI Baxter, Ivan/A-1052-2009 OI Baxter, Ivan/0000-0001-6680-1722 NR 15 TC 3 Z9 3 U1 1 U2 21 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 1940-3372 J9 PLANT GENOME-US JI Plant Genome PD JUL PY 2013 VL 6 IS 2 DI 10.3835/plantgenome2012.07.0012 PG 9 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA 197YC UT WOS:000322887700003 ER PT J AU Campbell, JL Rustad, LE Porter, JH Taylor, JR Dereszynski, EW Shanley, JB Gries, C Henshaw, DL Martin, ME Sheldon, WM Boose, ER AF Campbell, John L. Rustad, Lindsey E. Porter, John H. Taylor, Jeffrey R. Dereszynski, Ethan W. Shanley, James B. Gries, Corinna Henshaw, Donald L. Martin, Mary E. Sheldon, Wade M. Boose, Emery R. TI Quantity is Nothing without Quality: Automated QA/QC for Streaming Environmental Sensor Data SO BIOSCIENCE LA English DT Article DE computers in biology; informatics; instrumentation; environmental science ID ASSURANCE PROCEDURES; OKLAHOMA MESONET; NETWORKS; UNCERTAINTY; ECOLOGY; SYSTEM; TEMPERATURE; SCALE; FLUX AB Sensor networks are revolutionizing environmental monitoring by producing massive quantities of data that are being made publically available in near real time. These data streams pose a challenge for ecologists because traditional approaches to quality assurance and quality control are no longer practical when confronted with the size of these data sets and the demands of real-time processing. Automated methods for rapidly identifying and (ideally) correcting problematic data are essential. However, advances in sensor hardware have outpaced those in software, creating a need for tools to implement automated quality assurance and quality control procedures, produce graphical and statistical summaries for review, and track the provenance of the data. Use of automated tools would enhance data integrity and reliability and would reduce delays in releasing data products. Development of community-wide standards for quality assurance and quality control would instill confidence in sensor data and would improve interoperability across environmental sensor networks. C1 [Campbell, John L.; Rustad, Lindsey E.] US Forest Serv, No Res Stn, USDA, Durham, NH USA. [Porter, John H.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22903 USA. [Taylor, Jeffrey R.] Natl Ecol Observ Network, Boulder, CO USA. [Dereszynski, Ethan W.] Oregon State Univ, Dept Comp Sci, Corvallis, OR 97331 USA. [Shanley, James B.] US Geol Survey, New England Water Sci Ctr, Montpelier, VT USA. [Gries, Corinna] Univ Wisconsin Madison, Ctr Limnol, Madison, WI USA. [Henshaw, Donald L.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Martin, Mary E.] Univ New Hampshire, Complex Syst Res Ctr, Durham, NH 03824 USA. [Sheldon, Wade M.] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA. [Boose, Emery R.] Harvard Univ Harvard Forest, Petersham, MA USA. RP Campbell, JL (reprint author), US Forest Serv, No Res Stn, USDA, Durham, NH USA. EM jlcampbell@fs.fed.us OI Gries, Corinna/0000-0002-9091-6543; Campbell, John/0000-0003-4956-1696 FU Northeastern States Research Cooperative; National Science Foundation (NSF) [DEB-0832652]; NSF [DEB-0620443, DEB-1237733, DEB-0822700, DEB-0823380, DEB-1114804, OCE-0620959] FX The authors would like to thank David Hollinger and Nicholas Grant for providing helpful comments on an earlier draft of this manuscript. Funding was provided by the Northeastern States Research Cooperative and the National Science Foundation (NSF) through a cooperative agreement to the US Long Term Ecological Research Network Office (grant no. DEB-0832652) and NSF grants no. DEB-0620443, no. DEB-1237733, no. DEB-0822700, no. DEB-0823380, no. DEB-1114804, and no. OCE-0620959. NR 46 TC 13 Z9 13 U1 5 U2 35 PU AMER INST BIOLOGICAL SCI PI WASHINGTON PA 1444 EYE ST, NW, STE 200, WASHINGTON, DC 20005 USA SN 0006-3568 J9 BIOSCIENCE JI Bioscience PD JUL PY 2013 VL 63 IS 7 BP 574 EP 585 DI 10.1525/bio.2013.63.7.10 PG 12 WC Biology SC Life Sciences & Biomedicine - Other Topics GA 194EI UT WOS:000322613400010 ER PT J AU Beasley, RR Pijut, PM AF Beasley, Rochelle R. Pijut, Paula M. TI Regeneration of Plants from Fraxinus nigra Marsh. Hypocotyls SO HORTSCIENCE LA English DT Article DE adventitious shoots; black ash; rooting; shoot organogenesis; vitro ID IN-VITRO PROPAGATION; SHOOT ORGANOGENESIS; THIDIAZURON; MINNESOTA; GROWTH; COTYLEDONS; EXCELSIOR; CULTURES; DIEBACK; L. AB Fraxinus nigra Marsh. (black ash) is a native North American hardwood tree species that is ecologically important and has ethnobotanical significance to American Indian communities of the eastern United States. Black ash has immature embryos at seed set, combined with complex stratification requirements, making natural regeneration difficult. This, combined with the threat and devastation being caused by the emerald ash borer, makes an in vitro adventitious shoot regeneration and rooting protocol imperative for mass propagation, conservation, and genetic improvement of this species. Hypocotyls were cultured for 4 weeks on a modified Murashige and Skoog (MS) medium containing 13.3 mu M 6-benzylaminopurine (BA) and 4.5 mu M thidiazuron for adventitious shoot induction. Shoots continued to regenerate when explants were then cultured on MS medium supplemented with Gamborg B5 vitamins plus 0.2 g.L-1 glycine (B5G), 6.7 mu M BA, 1 mu M indole-3-butryic acid (IBA), and 0.29 mu M gibberellic acid (GA(3)) for 4 weeks, followed by transfer to MSB5G medium with 13.3 mu M BA, 1 mu M IBA, and 0.29 mu M GA(3) for shoot elongation. Once elongated, the microshoots were continuously micropropagated through nodal sectioning, and cultured on MSB5G medium supplemented with 13.3 mu M BA, 1 mu M IBA, 0.29 mu M GA(3), and 0.2 g.L-1 casein hydrolysate. Rooting of elongated microshoots was successful using woody plant medium supplemented with 4.9 mu M IBA and 5.7 mu M indole-3-acetic acid with a 10-day initial dark culture for root induction followed by culture under a 16-h photoperiod. Rooted plantlets were successfully acclimatized to the greenhouse with 100% survival. C1 [Beasley, Rochelle R.] Purdue Univ, Dept Forestry & Nat Resources, Hardwood Tree Improvement & Regenerat Ctr, W Lafayette, IN 47907 USA. [Pijut, Paula M.] US Forest Serv, USDA, No Res Stn, HTIRC, W Lafayette, IN 47907 USA. RP Pijut, PM (reprint author), US Forest Serv, USDA, No Res Stn, HTIRC, 715 West State St, W Lafayette, IN 47907 USA. EM ppijut@purdue.edu RI Beasley, Rochelle/M-1396-2015; Pijut, Paula/N-6789-2015 OI Beasley, Rochelle/0000-0001-7325-4085; NR 30 TC 4 Z9 4 U1 1 U2 13 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUL PY 2013 VL 48 IS 7 BP 887 EP 890 PG 4 WC Horticulture SC Agriculture GA 193LP UT WOS:000322561600012 ER PT J AU Pooler, MR AF Pooler, Margaret R. TI Loropetalum chinense 'Snow Panda' SO HORTSCIENCE LA English DT Editorial Material DE Chinese fringe flower; plant exploration; plant introduction C1 USDA ARS, US Natl Arboretum, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Pooler, MR (reprint author), USDA ARS, US Natl Arboretum, Floral & Nursery Plants Res Unit, 10300 Baltimore Ave,Bldg 010A, Beltsville, MD 20705 USA. EM Margaret.Pooler@ars.usda.gov NR 3 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUL PY 2013 VL 48 IS 7 BP 906 EP 906 PG 1 WC Horticulture SC Agriculture GA 193LP UT WOS:000322561600016 ER PT J AU Farnham, MW AF Farnham, Mark W. TI Doubled Haploid Inbred Lines USVL048 and USVL131 of Heading Broccoli SO HORTSCIENCE LA English DT Editorial Material DE Brassica oleracea-Italica group; doubled haploids; cole crops; anther culture ID HORTICULTURAL TRAITS C1 USDA ARS, US Vegetable Lab, Charleston, SC 29414 USA. RP Farnham, MW (reprint author), USDA ARS, US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Mark.Farnham@ars.usda.gov NR 6 TC 0 Z9 0 U1 1 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 J9 HORTSCIENCE JI Hortscience PD JUL PY 2013 VL 48 IS 7 BP 907 EP 908 PG 2 WC Horticulture SC Agriculture GA 193LP UT WOS:000322561600017 ER PT J AU Eggleston, G Viator, R Gateuil, A Fenger, JA White, P Jackson, W Waguespack, H Blackwelder, N AF Eggleston, G. Viator, R. Gateuil, A. Fenger, J-A. White, P. Jackson, W. Waguespack, H., Jr. Blackwelder, N. TI Effects of seasonal variations of sugarcane stalk and extraneous matter quantity and quality as they affect recoverable sugar, starch and fiber: Part I SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE extraneous matter; fiber; recoverable sugar; seasonal quality variations; starch; sugarcane ID TRASH AFFECTS PRODUCTION; GREEN CANE BILLETS; REGISTRATION; INTERMEDIATE AB There are presently worldwide trends to manufacture high quality raw sugars for supply to refineries, e.g., very high pol and very low color (VHPNLC) raw sugars, and to use sugarcane extraneous matter (trash or leaves and tops) as a source of biomass. Raw sugar quality is dependent on the quality of the incoming cane supply across the processing season which, in turn, is greatly affected by the quantities and types of non-stalk tissues present. Strategies to improve the quality of raw sugar will require attention to seasonal cane quality factors as well as process manipulations. This study was undertaken to ascertain seasonal cane supply variations in juice quality parameters that affect raw sugar manufacture across the Louisiana (LA) 3-month processing season (late Sept to Dec) in 2009 and 2010. Across both seasons, maximum daily air temperature decreased with little difference in the average values (ay. 23.3 degrees C), but there was consistently higher daily rainfall in 2009 (ay. 13.7 mm) than 2010 (ay. 1.8 mm). Juice was extracted from separated stalk (S), growing point region (GPR or top), green leaf (GL) and brown leaf (BL) tissues of three commercial sugarcane varieties: HoCP 96-540, L 99-226, and L 99-233, at least five times between Sept and Dec. For all three varieties across harvest dates, the average total trash value (GPR + GL + BL) was 20.6% on wet weight. The most popular LA variety HoCP 96-540, still had relatively high levels of total trash until late Nov. There was a significant (P<.05) harvest date by varietal interaction for theoretical recovery of sugar in stalk. The maturity characteristic of the variety played a critical role in seasonal variation of quality parameters, especially starch. Compared to early and mid-maturing varieties, L99-226 and HoCP 96-540, respectively, which mostly delivered starch to the factories in green leaves, the later maturing variety L99-233 delivered starch mostly in the stalk after mid Oct. Thus blowing off extraneous matter in the field or at the factory for L99-233 would help little to control starch levels after mid Oct The greatest (P<.05) variation in harvest date effect on starch concentration was in the stalk, and there was also a very strong varietal effect on starch. In contrast, the least varietal variation in juice quality parameters occurred in the GPR. Fiber did not vary considerably across the season for all varieties. Overall, for all parameters studied the type of tissue had the strongest effect, followed by variety, then date of harvest; the varietal effect was greatest in the stalk than other tissues. This suggests that breeding programs could include quality parameters as selection criteria. C1 [Eggleston, G.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Viator, R.; White, P.] USDA ARS, Sugarcane Unit, Houma, LA 70360 USA. [Gateuil, A.; Fenger, J-A.] Agro Paris Tech, Paris, France. [Jackson, W.; Waguespack, H., Jr.; Blackwelder, N.] Amer Sugar Cane League, Thibodaux, LA 70302 USA. RP Eggleston, G (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM gillian.eggleston@ars.usda.gov NR 29 TC 8 Z9 8 U1 0 U2 7 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD JUL PY 2013 VL 115 IS 1375 BP 477 EP 487 PG 11 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA 191NT UT WOS:000322420600036 ER PT J AU Forshage, M Nordlander, G Buffington, ML AF Forshage, Mattias Nordlander, Goran Buffington, Matthew L. TI EUCOILINAE OF NORTH AMERICA: A REVISED CATALOG OF GENERA AND DESCRIBED SPECIES SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Tetramerocera; Ganaspis; Bewelda; Aporeucoela; Hexacola; Pentaplastidia; Trybliographa; Figitidae; Cynipoidea ID HYMENOPTERA FIGITIDAE EUCOILINAE; AGROSTOCYNIPS-DIAZ HYMENOPTERA; CYNIPOIDEA; GENUS; FORSTER; BUFFINGTON; PHYLOGENY; CIRCUMSCRIPTION; EVOLUTION AB We present an updated catalog of North American Eucoilinae, bearing little resemblance to previous regional catalogs, which have been lagging behind in the recent systematic work in the group. The current catalog comprises 34 genera, arranged in six tribes. Of these genera, 9 are represented wholly by unidentified or undescribed species in the region, while the other 25 include 108 species recorded from the region. In comparison with previous catalogs, 24 genera and 41 species are added, and 34 species-level names are recombined, while 7 genera, five subgenera and 11 species are removed from the list of North American taxa. A modern, phylogenetically stable and type-based classification is implemented, as well as a tribal classification. In terms of nomenclatural acts, 25 new combinations are made (one is a reinstatement of an old combination); four new genus-levels synonymies are made (Tetramerocera Ashmead junior synonym of Ganaspis Forster, Bewelda Quinlan and Aporeucoela Kieffer junior synonyms of Hexacola Forster, Pentaplastidia Weld junior synonym of Trybliographa Forster); eight new species-level synonymies are made; two species names are removed from synonymy; one new replacement name given (Hexacola pennsylvanicus for Hexacola subaperta Kieffer 1907 nec (Kieffer 1901a)). C1 [Forshage, Mattias] Swedish Museum Nat Hist, Dept Entomol, SE-10405 Stockholm, Sweden. [Nordlander, Goran] Swedish Univ Agr Sci, Dept Ecol, SE-75007 Uppsala, Sweden. [Buffington, Matthew L.] ARS, Systemat Entomol Lab, USDA, Smithsonian Inst,Natl Museum Nat Hist, Washington, DC 20560 USA. RP Forshage, M (reprint author), Swedish Museum Nat Hist, Dept Entomol, Box 50007, SE-10405 Stockholm, Sweden. EM Mattias.Forshage@nrm.se; Goran.Nordlander@slu.se; matt.buffington@ars.usda.gov NR 130 TC 3 Z9 3 U1 0 U2 8 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2013 VL 115 IS 3 BP 225 EP 255 DI 10.4289/0013-8797.115.3.225 PG 31 WC Entomology SC Entomology GA 194CJ UT WOS:000322608300001 ER PT J AU Gates, MW Hanson, PE AF Gates, Michael W. Hanson, Paul E. TI NEW ISOSOMODES (HYMENOPTERA: EURYTOMIDAE) AND IMPLICATIONS FOR BIOLOGICAL CONTROL IN RICE (ORYZA SATIVAE; POACEAE) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Chalcidoidea; egg parasitoids; Tettigoniidae AB Two new species of Isosomodes Ashmead are described and illustrated: I. monteria from Colombia and I. dorado from Venezuela. Key characters for separation from other species are provided. Rearing data for I. monteria indicate that it is a parasitoid of Conocephalus sp. (Orthoptera: Tettigoniidae) eggs and its importance in biological control programs in rice are discussed. C1 [Gates, Michael W.] ARS, Systemat Entomol Lab, USDA, PSI,Natl Museum Nat Hist, Washington, DC 20013 USA. [Hanson, Paul E.] Univ Costa Rica, Escuela Biol, San Jose, Costa Rica. RP Gates, MW (reprint author), ARS, Systemat Entomol Lab, USDA, PSI,Natl Museum Nat Hist, MRC 168, Washington, DC 20013 USA. EM michael.gates@ars.usda.gov; phanson@biologia.ucr.ac.cr FU Global Environment Facility; Universidad de Costa Rica FX We would like to thank Enrique Saavedra de Castro (FEDEARROZ, Colombia) and the Gary Gibson at CNC for providing specimens. We also acknowledge Alma Solis (U.S. Department of Agriculture, Systematic Entomology Laboratory) for reviewing this manuscript. PEH thanks the Global Environment Facility and the Universidad de Costa Rica for financial support to visit the NMNH. USDA is an equal opportunity employer. NR 7 TC 0 Z9 0 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2013 VL 115 IS 3 BP 256 EP 264 DI 10.4289/0013-8797.115.3.256 PG 9 WC Entomology SC Entomology GA 194CJ UT WOS:000322608300002 ER PT J AU Lingafelter, SW Ivle, MA AF Lingafelter, Steven W. Ivle, Michael A. TI A NEW GENUS OF MIMETIC LONGHORNED BEETLE FROM ST. LUCIA, LESSER ANTILLES (COLEOPTERA: CERAMBYCIDAE: RHINOTRAGINI) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Endemic; wood-borer; mimicry; taxonomy; identification AB A species originally described as Fortuneleptura romei Touroult, 2011 (Lepturinae) is placed in a new genus, Iyanola Lingafelter & Ivie (Cerambycinae: Rhinotragini). Along with the new genus description, the species is redescribed and additional collection data is recorded. A key to the genera and species of Rhinotragini (Cerambycidae: Cerambycinae) of the West Indies is presented. C1 [Lingafelter, Steven W.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist, Washington, DC 20013 USA. [Ivle, Michael A.] Montana State Univ, Marsh Labs, Montana Entomol Collect MSU, Bozeman, MT 59717 USA. RP Lingafelter, SW (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist, MRC 168,POB 37012, Washington, DC 20013 USA. EM steve.lingafelter@ars.usda.gov NR 8 TC 1 Z9 1 U1 0 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD JUL PY 2013 VL 115 IS 3 BP 268 EP 273 DI 10.4289/0013-8797.115.3.268 PG 6 WC Entomology SC Entomology GA 194CJ UT WOS:000322608300004 ER PT J AU Reeves, JL Derner, JD Sanderson, MA Petersen, MK Vermeire, LT Hendrickson, JR Kronberg, SL AF Reeves, Justin L. Derner, Justin D. Sanderson, Matt A. Petersen, Mark K. Vermeire, Lance T. Hendrickson, John R. Kronberg, Scott L. TI Temperature and Precipitation Affect Steer Weight Gains Differentially by Stocking Rate in Northern Mixed-Grass Prairie SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE beef cattle production; climate variability; decision support tools; grazing management; model averaging; semiarid rangeland ID CLIMATE VARIABILITY; ECONOMIC RESPONSES; FORAGE PRODUCTION; GRAZING SYSTEMS; CATTLE; SHORTGRASS; VEGETATION; SIMULATION; STRATEGIES; QUALITY AB Cattle weight gain responses to seasonal weather variability are difficult to predict for rangelands because few long-term (>20 yr) studies have been conducted. However, an increased understanding of temperature and precipitation influences on cattle weight gains is needed to optimize stocking rates and reduce enterprise risk associated with climatic variability. Yearling steer weight gain data collected at the USDA-ARS High Plains Grasslands Research Station at light, moderate, and heavy stocking rates for 30 years (1982-2011) were used to examine the effects of spring (April-June) and summer (July-September) temperature and precipitation, as well as prior-growing-season (prior April-September) and fall/winter (October-March) precipitation, on beef production (kg . ha(-1)). At heavier stocking rates, steer production was more sensitive to seasonal weather variations. A novel finding was that temperature (relatively cool springs and warm summers) played a large predictive role on beef production. At heavier stocking rates, beef production was highest during years with cool, wet springs and warm, wet summers, corresponding to optimum growth conditions for this mixed C-3-C-4 plant community. The novelty and utility of these findings may increase the efficacy of stocking rate decision support tools. The parsimonious model structure presented here includes three-month seasonal clusters that are forecasted and freely available from the US National Oceanic and Atmospheric Administration up to a year in advance. These seasonal weather forecasts can provide ranchers with an increased predictive capacity to adjust stocking rates (in advance of the grazing season) according to predicted seasonal weather conditions, thereby reducing enterprise risk. C1 [Reeves, Justin L.; Derner, Justin D.] USDA ARS, Rangeland Resources Res Unit, Cheyenne, WY 82009 USA. [Sanderson, Matt A.; Hendrickson, John R.; Kronberg, Scott L.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Petersen, Mark K.; Vermeire, Lance T.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Reeves, JL (reprint author), USDA ARS, High Plains Grassland Res Stn, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. EM Justin.Reeves@ars.usda.gov NR 37 TC 7 Z9 7 U1 0 U2 10 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 EI 1551-5028 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD JUL PY 2013 VL 66 IS 4 BP 438 EP 444 DI 10.2111/REM-D-12-00157.1 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 191KW UT WOS:000322412900006 ER PT J AU Davies, KW Boyd, CS Nafus, AM AF Davies, Kirk W. Boyd, Chad S. Nafus, Aleta M. TI Restoring the Sagebrush Component in Crested Wheatgrass-Dominated Communities SO RANGELAND ECOLOGY & MANAGEMENT LA English DT Article DE Agropyron cristatum; Artemisia tridentata; establishment; restoration; seedings ID WYOMING BIG SAGEBRUSH; PLANT-COMMUNITIES; GREAT-BASIN; PERENNIAL GRASS; SOUTHERN IDAHO; NATIVE PLANTS; ESTABLISHMENT; PRAIRIE; STANDS; SASKATCHEWAN AB Monotypic stands of crested wheatgrass (Agropyron cristatum [L] Gaertm. and Agropyron desertorum [Fisch.] Schult.), an introduced grass, occupy vast expanses of the sagebrush steppe. Efforts to improve habitat for sagebrush-associated wildlife by establishing a diverse community of native vegetation in crested wheatgrass stands have largely failed. Instead of concentrating on. a diversity of species, we evaluated the potential to restore the foundation species, Wyoming big sagebrush (Artemisia tridentata spp. wyomingensis [Beetle & A. Young] S. L. Welsh), to these communities. We investigated the establishment of Wyoming big sagebrush into six crested wheatgrass stands (sites) by broadcast seeding and planting seedling sagebrush across varying levels of crested wheatgrass control with glyphosate. Planted sagebrush seedlings survived at high rates (similar to 70% planted sagebrush survival 3 yr postplanting), even without crested wheatgrass control. However, most attempts to establish sagebrush by broadcast seeding failed. Only at high levels of crested wheatgrass control did a few sagebrush plants establish from broadcasted seed. Sagebrush density and cover were greater with planting seedlings than broadcast seeding. Sagebrush cover, height, and canopy area were greater at higher levels of crested wheatgrass control. High levels of crested wheatgrass control also created an opportunity for exotic annuals to increase. Crested wheatgrass rapidly recovered after glyphosate control treatments, which suggests multiple treatments may be needed to effectively control crested wheatgrass. Our results suggest that planting sagebrush seedlings can structurally diversify monotypic crested wheatgrass stands to provide habitat for sagebrush-associated wildlife. Though this is not the full diversity of native functional groups representative of the sagebrush steppe, it is a substantial improvement over other efforts that have largely failed to alter these plant communities. We also hypothesize that planting sagebrush seedlings in patches or strips may provide a relatively inexpensive method to facilitate sagebrush recovery across vast landscapes where sagebrush has been lost. C1 [Davies, Kirk W.; Boyd, Chad S.] USDA ARS, Burns, OR 97720 USA. [Nafus, Aleta M.] Oregon State Univ, Burns, OR 97720 USA. RP Davies, KW (reprint author), 67826-A Hwy 205, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu NR 43 TC 17 Z9 17 U1 1 U2 34 PU SOC RANGE MANAGEMENT PI LAKEWOOD PA 445 UNION BLVD, STE 230, LAKEWOOD, CO 80228-1259 USA SN 1550-7424 J9 RANGELAND ECOL MANAG JI Rangel. Ecol. Manag. PD JUL PY 2013 VL 66 IS 4 BP 472 EP 478 DI 10.2111/REM-D-12-00145.1 PG 7 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA 191KW UT WOS:000322412900010 ER PT J AU Rudie, AW AF Rudie, Alan W. TI TAPPI Journal showcases the best of PEERS 2012 SO TAPPI JOURNAL LA English DT Editorial Material C1 USDA, Forest Prod Lab, Madison, WI 53705 USA. RP Rudie, AW (reprint author), USDA, Forest Prod Lab, Madison, WI 53705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD JUL PY 2013 VL 12 IS 7 BP 5 EP 6 PG 2 WC Materials Science, Paper & Wood SC Materials Science GA 191VW UT WOS:000322443400001 ER PT J AU Hart, PW Houtman, C Hirth, K AF Hart, Peter W. Houtman, Carl Hirth, Kolby TI Hydrogen peroxide and caustic soda: Dancing with a dragon while bleaching SO TAPPI JOURNAL LA English DT Article ID STABILIZATION AB When hydrogen peroxide is mixed with caustic soda, an auto-accelerating reaction can lead to generation of significant amounts of heat and oxygen. On the basis of experiments using typical pulp mill process concentration and temperatures, a relatively simple kinetic model has been developed. Evaluation of these model results reveals that hydrogen peroxide-caustic soda systems are extremely sensitive to hydrogen peroxide: caustic soda ratio, transition metal contamination, and temperature. Small changes in initial conditions can result in a closed system becoming explosive. Analysis of model results was used to develop guidelines for safer application of hydrogen peroxide in a mill setting. C1 [Hart, Peter W.] MWV Corp, New Technol, Atlanta, GA USA. [Houtman, Carl; Hirth, Kolby] USDA, Forest Prod Lab, Madison, WI 53705 USA. RP Hart, PW (reprint author), MWV Corp, New Technol, Atlanta, GA USA. EM peter.hart@mwv.com NR 21 TC 2 Z9 2 U1 0 U2 0 PU TECH ASSOC PULP PAPER IND INC PI NORCROSS PA 15 TECHNOLOGY PARK SOUTH, NORCROSS, GA 30092 USA SN 0734-1415 J9 TAPPI J JI TAPPI J. PD JUL PY 2013 VL 12 IS 7 BP 59 EP 65 PG 7 WC Materials Science, Paper & Wood SC Materials Science GA 191VW UT WOS:000322443400006 ER PT J AU Peterson, E Grant, J Roberts, D Karov, V AF Peterson, Everett Grant, Jason Roberts, Donna Karov, Vuko TI Evaluating the Trade Restrictiveness of Phytosanitary Measures on US Fresh Fruit and Vegetable Imports SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE fruit and vegetable trade; phytosanitary treatments; non-tariff measures; gravity equation; Poisson; Zero-Inflated Poisson; F13; Q17 ID FOOD SAFETY STANDARDS; INTERNATIONAL-TRADE; GRAVITY; REGULATIONS; BARRIERS; IMPACT; MODEL AB Empirically assessing sanitary and phytosanitary regulations has proven difficult because most data sources indicate whether a regulation exists but provide no information on the type or importance of the respective measure. In this article, we construct a novel database of U.S. phytosanitary measures and match these to 47 fresh fruit and vegetable product imports from 89 exporting countries over the period 1996-2008. A product-line gravity equation that accounts for zero trade flows is developed to investigate the trade impact of different pest-mitigation measures. While the results suggest that phytosanitary treatments generally reduce trade, the actual restrictiveness of these measures diminishes dramatically as exporters accumulate experience, and it vanishes when exporters reach a certain threshold. The results have important policy implications considering the number of empirical studies that find a negative impact of non-tariff measures on trade. C1 [Peterson, Everett; Grant, Jason] Virginia Tech, Dept Agr & Appl Econ, Blacksburg, VA 24061 USA. [Roberts, Donna] Econ Res Serv, USDA, Market & Trade Econ Div, Washington, DC USA. [Karov, Vuko] Univ Arkansas, Dept Agr Econ & Agribusiness, Fayetteville, AR 72701 USA. RP Peterson, E (reprint author), Virginia Tech, Dept Agr & Appl Econ, Blacksburg, VA 24061 USA. EM petrsone@vt.edu FU Department of Agricultural and Applied Economics at Virginia Tech; Economic Research Service [43-EAEL5-80055]; National Institute of Food and Agriculture's (NIFA) Agricultural and Food Research Initiative (AFRI) [2010-65400-20437] FX Peterson is a professor, and Grant is an assistant professor in the Department of Agricultural and Applied Economics at Virginia Tech. Roberts is Associate Director of the Market and Trade Economics Division at the Economic Research Service of the U.S. Department of Agriculture. Karov is an extension specialist in the Department of Agricultural Economics & Agribusiness at the University of Arkansas. The authors wish to thank two anonymous reviewers and the editor, David Hennessy, for helpful comments. We also received many helpful suggestions from John Beghin, David Orden, and seminar participants at the 2011 annual meeting of the American Agricultural and Applied Economics Association, the 2011 annual meeting of the International Agricultural Trade Research Consortium, and Staff Economists at the Animal and Plant Health Inspection Service (APHIS) and the U.S. International Trade Commission. Grant, Peterson, and Roberts wish to acknowledge financial support provided by the Economic Research Service under cooperative agreement 43-EAEL5-80055 and the National Institute of Food and Agriculture's (NIFA) Agricultural and Food Research Initiative (AFRI) Grant 2010-65400-20437. The views expressed here are those of the authors and may not be attributed to the U.S. Department of Agriculture or the Economic Research Service. Correspondence may be sent to: petrsone@vt.edu. NR 37 TC 4 Z9 4 U1 6 U2 37 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD JUL PY 2013 VL 95 IS 4 BP 842 EP 858 DI 10.1093/ajae/aat015 PG 17 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA 186DN UT WOS:000322022300003 ER PT J AU Mullen, CA Boateng, AA Goldberg, NM AF Mullen, Charles A. Boateng, Akwasi A. Goldberg, Neil M. TI Production of Deoxygenated Biomass Fast Pyrolysis Oils via Product Gas Recycling SO ENERGY & FUELS LA English DT Article ID CATALYTIC FAST PYROLYSIS; BIO-OIL; HYDROPYROLYSIS; GASOLINE; LIQUIDS; FUELS AB A bench scale fluidized bed system was modified to recycle and utilize the gaseous products of biomass fast pyrolysis as fluidization gas and to create a reactive gas atmosphere to replace, in part or in full, the added nitrogen gas. The effect of the presence of the reducing atmosphere on the pyrolysis oils produced was studied for three different biomass feedstocks, white oak, switchgrass, and pennycress presscake, and compared with those produced under an inert N-2 atmosphere as the control. The reductive atmosphere, consisting primarily of CO, CO2, H-2, and light hydrocarbons, had a significant deoxygenation effect on the pyrolysis oil produced from oak and switchgrass. The effect was significantly smaller for the pyrolysis of pennycress presscake, which inherently produces a lower oxygen product. When oak and switchgrass were used as feedstocks, pyrolysis oils with molar C/O ratios of 9.1 and 8.5 were achieved, respectively, which is increased from about 2.1 for the control experiments for each feedstock. The pyrolysis oils produced under the reducing atmosphere were richer in aromatic hydrocarbons and nonmethoxylated phenolics and had lesser concentrations of levoglucosan and acids than were the pyrolysis oils in the control experiments. These pyrolysis oils had lower total acid numbers (TAN) and higher energy content than those produced in the control experiments. These results compare favorably to zeolite catalyzed fast pyrolysis but without the added catalyst cost and concern for catalyst deactivation or need for regeneration. C1 [Mullen, Charles A.; Boateng, Akwasi A.; Goldberg, Neil M.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Mullen, CA (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov NR 19 TC 27 Z9 27 U1 2 U2 42 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD JUL PY 2013 VL 27 IS 7 BP 3867 EP 3874 DI 10.1021/ef400739u PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 187VS UT WOS:000322150200030 ER PT J AU Jackson, MA AF Jackson, Michael A. TI Ketonization of Model Pyrolysis Bio-oil Solutions in a Plug-Flow Reactor over a Mixed Oxide of Fe, Ce, and Al SO ENERGY & FUELS LA English DT Article ID CARBOXYLIC-ACIDS; ACETIC-ACID; CONDENSATION-REACTIONS; CATALYSTS; KETONES; CERIA; REDUCTION; ESTERS AB The stabilization and upgrading of pyrolysis oil requires the neutralization of the acidic components of the oil. The conversion of small organic acids, particularly acetic acid, to ketones is one approach to addressing the instability of the oils caused by low pH. In the ketonization reaction, acetic acid is converted to acetone, water, and CO2. Here, a 16 vol % acetic acid solution is converted to acetone in a flow reactor over the mixed oxide Fe0.2Ce0.2Al0.6Ox in the presence of several other components meant to represent pyrolysis oil. These components include hydroxyacetone, furfural, phenol, cresol, guaiacol, and eugenol. Hydroxyacetone also undergoes ketonization, forming acetone, 2-butanone, 3-pentanone, and 2- and 3-hexanone. A mechanism for the ketonization of hydroxyacetone through propanal is proposed and supported by incorporation of isotopically labeled water. Inhibition of the reaction occurs to a significant degree by the addition of furfural, guaiacol, and eugenol. C1 USDA ARS, Renewable Prod Technol Unit, NCAUR, Peoria, IL 61604 USA. RP Jackson, MA (reprint author), USDA ARS, Renewable Prod Technol Unit, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM michael.jackson@ars.usda.gov NR 32 TC 7 Z9 7 U1 4 U2 40 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 J9 ENERG FUEL JI Energy Fuels PD JUL PY 2013 VL 27 IS 7 BP 3936 EP 3943 DI 10.1021/ef400789z PG 8 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA 187VS UT WOS:000322150200038 ER PT J AU Nayduch, D Joyner, C AF Nayduch, Dana Joyner, Chester TI Expression of Lysozyme in the Life History of the House Fly (Musca domestica L.) SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE stage-specific expression; tissue-specific expression; Staphylococcus aureus ID DIGESTIVE ENZYMES; IMMUNE-RESPONSE; LARVAE; DIPTERA; BACTERIA; MIDGUT; TRACT; PEPTIDOGLYCAN; PURIFICATION; PROTEIN AB From egg to adult, all life history stages of house flies associate with septic environments teeming with bacteria. House fly lysozyme was first identified in the larval midgut, where it is used for digestion of microbe-rich meals because of its broad-spectrum activity against gram-positive and gram-negative bacteria as well as fungi. This study aimed to determine the temporal expression of lysozyme in the life history of house flies ( from egg through adults) on both the mRNA and protein level, and to determine the tissue-specific expression of lysozyme in adult flies induced by feeding Staphylococcus aureus. From 30-min postoviposition through adulthood, all life history stages of the house fly express lysozyme on the mRNA level. In adult flies, lysozyme is expressed both locally in the alimentary canal and systemically in the fat body. Interestingly, we found that during the normal life history of flies, lysozyme protein was only detected in larval stages and older adults, likely because of ingestion of immune-stimulating levels of bacteria, not experienced during egg, pupa, and teneral adult stages. Constitutive expression on the mRNA level implies that this effector is a primary defense molecule in all stages of the house fly life history, and that a mechanism for posttranscriptional control of mature lysozyme enzyme expression may be present. Lysozyme active enzyme primarily serves both a digestive and defensive function in larval and adult flies, and may be a key player in the ability of Musca domestica L. to thrive in microbe-rich environments. C1 [Nayduch, Dana; Joyner, Chester] Georgia So Univ, Dept Biol, Statesboro, GA 30460 USA. RP Nayduch, D (reprint author), USDA ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. EM dana.nayduch@ars.usda.gov FU R15 Academic Research Enhancement Award (AREA) from the National Institutes of Health [1R15AI084029-01] FX We thank C. Evett for assistance with RT-PCR and specimen collection. Staphylococcus aureus was kindly provided by Albert Cheung, Dartmouth University. This work was supported by R15 Academic Research Enhancement Award (AREA) 1R15AI084029-01 from the National Institutes of Health awarded to D.N. NR 29 TC 6 Z9 6 U1 1 U2 15 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-2585 J9 J MED ENTOMOL JI J. Med. Entomol. PD JUL PY 2013 VL 50 IS 4 BP 847 EP 852 DI 10.1603/ME12167 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 184NI UT WOS:000321897300022 PM 23926784 ER PT J AU Obenauer, PJ Abdel-Dayem, MS Stoops, CA Villinski, JT Tageldin, R Fahmy, NT Diclaro, JW Bolay, F AF Obenauer, P. J. Abdel-Dayem, M. S. Stoops, C. A. Villinski, J. T. Tageldin, R. Fahmy, N. T. Diclaro, J. W., II Bolay, F. TI Field Responses of Anopheles gambiae Complex (Diptera: Culicidae) in Liberia using Yeast-Generated Carbon Dioxide and Synthetic Lure-Baited Light Traps SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE mosquito; UV; light-emitting diode; Malaria; S-form ID MM-X TRAPS; WESTERN KENYA; IDENTIFICATION; ATTRACTION; ARABIENSIS; MOSQUITOES; ODOR AB Malaria infection is a serious public health problem throughout Liberia, but vector surveillance is limited or nonexistent in remote regions of the country. To better understand the spatial and temporal distribution of malaria vectors in Liberia and to support vector and malaria activities of the Liberian Ministry of Health, a study was conducted to determine the efficacy of light traps baited with a synthetic lure and CO 2 for capturing Anopheles gambiae sensu lato (Giles). Traps with a ultraviolet, light-emitting diode, and incandescent lights baited with a synthetic skin lure and CO 2 combinations were evaluated at four field sites in three counties of Liberia for five consecutive nights every 8 wk during 2011. In total, 4,788 mosquitoes representing 56 species from nine genera were collected throughout the 30-wk study; An. gambiae s. l. comprised 32% and of the 148 An. gambiae s. s. collected, 85% were of the S form. A greater percentage of An. gambiae s. l. were collected in ultraviolet traps baited with a synthetic lure and CO2 compared with any other trap configuration. The influence of trap configuration on conclusions from surveillance efforts, specifically with regards to An. gambiae is discussed. C1 [Obenauer, P. J.] USN, Entomol Ctr Excellence, Jacksonville, FL 32212 USA. [Abdel-Dayem, M. S.] King Saud Univ, Coll Food & Agr Sci, Riyadh 11451, Saudi Arabia. [Stoops, C. A.] USDA, Ctr Med & Vet Entomol, Gainesville, FL 32608 USA. [Villinski, J. T.; Tageldin, R.; Fahmy, N. T.; Diclaro, J. W., II] US Naval Med Res Unit 3, Cairo 09835, FPO AE, Egypt. [Bolay, F.] Liberian Inst Biomed Res, Monrovia, Liberia. RP Obenauer, PJ (reprint author), USN, Entomol Ctr Excellence, Box 43,Bldg 937, Jacksonville, FL 32212 USA. EM peter.obenauer@med.navy.mil OI Abdel-Dayem, Mahmoud/0000-0002-6276-1740 FU Department of Defense's Global Emerging Infections System (GEIS) work unit [C0436-11-N3, C0549-12-N3] FX We thank Maria Badra for her assistance with logistical support of all personnel and materials pertaining to this study. We thank Robyn Murrillo, Tony Hughes, Ryan Larson, Jesse Evans, Daniel Hanaczewski, Hanafi Hanafi, Maria Morales, Noha Watany, El-Shaimma Nour El-Din, and Emad Fawaz for assistance with collecting, processing, and identifying mosquito specimens. We are indebted to Jimmy Pitzer and Eric Hoffman who provided helpful suggestions and reviews of this manuscript. We are grateful to the LIBR support staff for assisting and executing this study. This work was funded by the Department of Defense's Global Emerging Infections System (GEIS) work unit numbers C0436-11-N3 and C0549-12-N3. The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the Department of the Navy, Department of Defense, nor the U.S. Government. NR 32 TC 10 Z9 10 U1 1 U2 11 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-2585 EI 1938-2928 J9 J MED ENTOMOL JI J. Med. Entomol. PD JUL PY 2013 VL 50 IS 4 BP 863 EP 870 DI 10.1603/ME12174 PG 8 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 184NI UT WOS:000321897300024 PM 23926786 ER PT J AU Tuckow, AP Temeyer, KB Olafson, PU De Leon, AAP AF Tuckow, Alexander P. Temeyer, Kevin B. Olafson, Pia U. De Leon, Adalberto A. Perez TI Discovery of MicroRNAs of the Stable Fly (Diptera: Muscidae) by High-Throughput Sequencing SO JOURNAL OF MEDICAL ENTOMOLOGY LA English DT Article DE miRNA; noncoding RNA; livestock pest; Stomoxys calcitrans ID STOMOXYS-CALCITRANS; CODING REGIONS; AEDES-AEGYPTI; EXPRESSION; DROSOPHILA; RESISTANCE; MIR-184; GENES; SITES; FLIES AB The stable fly, Stomoxys calcitrans (L.), is a serious ectoparasite affecting animal production and health of both animals and humans. Stable fly control relies largely on chemical insecticides; however, the development of insecticide resistance as well as environmental considerations requires continued discovery research to develop novel control technologies. MicroRNAs (miRNAs) are a class of short noncoding RNAs that have been shown to be important regulators of gene expression across a wide variety of organisms, and may provide an innovative approach with regard to development of safer more targeted control technologies. The current study reports discovery and initial comparative analysis of 88 presumptive miRNA sequences from the stable fly, obtained using high-throughput sequencing of small RNAs. The majority of stable fly miRNAs were 22-23 nt in length. Many miRNAs were arthropod specific, and several mature miRNA sequences showed greater sequence identity to miRNAs from other blood-feeding dipterans such as mosquitoes rather than to Drosophilids. This initial step in characterizing the stable fly microRNAome provides a basis for further analyses of life stage-specific and tissue-specific expression to elucidate their functional roles in stable fly biology. C1 [Tuckow, Alexander P.; Temeyer, Kevin B.; Olafson, Pia U.; De Leon, Adalberto A. Perez] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX USA. RP Temeyer, KB (reprint author), ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX USA. EM kevin.temeyer@ars.usda.gov FU USDA-ARS [6205-32000-033-00D]; USDA-ARS FX K. B. Temeyer, P. U. Olafson, and A. A. Perez de Leon are funded by USDA-ARS appropriated project 6205-32000-033-00D. A. P. Tuckow was supported by a USDA-ARS Postdoctoral Research Associate Program award to K. B. Temeyer. NR 40 TC 3 Z9 3 U1 0 U2 5 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0022-2585 J9 J MED ENTOMOL JI J. Med. Entomol. PD JUL PY 2013 VL 50 IS 4 BP 925 EP 930 DI 10.1603/ME12274 PG 6 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA 184NI UT WOS:000321897300032 PM 23926794 ER PT J AU Wilson, GV Fox, GA AF Wilson, G. V. Fox, G. A. TI Pore-Water Pressures Associated with Clogging of Soil Pipes: Numerical Analysis of Laboratory Experiments SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID PREFERENTIAL FLOW; SLOPE STABILITY; MACROPORES; PIPEFLOW; DYNAMICS; INITIATION; CATCHMENT; LANDSLIDE; EROSION AB Clogging of soil pipes due to excessive internal erosion has been hypothesized to cause extreme erosion events such as landslides, debris flows, and gullies, but confirmation of this phenomenon has been lacking. Laboratory and field measurements have failed to measure pore-water pressures within pipes and models of pipe flow have not addressed internal erosion or pipe clogging. The objective of this study was to model laboratory experiments of pipe flow in which clogging was observed in order to understand the clogging process. Richards' equation was used to model pipe flow, with the soil pipe represented as a highly conductive, low-retention porous medium. The modeling used two contrasting boundary conditions, constant flux (CF) and constant head (CH), to quantify pressure buildups due to pipe clogging and differences in simulated pressures between the two imposed boundary conditions. Unique to these simulations was inclusion of pipe enlargement with time due to internal erosion, representation of partially full flow conditions, and inclusion of pipe clogging. Both CF and CH boundary conditions confirmed the concept of pressure buildup as a result of pipe clogging. Pressure jumps of around 54 m for CF and 18 cm for CH occurred in <0.1 s, while soil water pressures 4 cm radially outward from the pipe had not responded. These findings demonstrate the need to measure pressures within soil pipes due to hydraulic nonequilibrium between the pipe and soil matrix. Pore water pressures within the pipe below the clog rapidly (<0.25 s) drained to unsaturated conditions, indicating the ability of soil pipes to drain hillslopes and rapidly recover when clogs are flushed from the soil pipe. These dynamic processes need to be incorporated into stability models to properly model hillslope processes. C1 [Wilson, G. V.] USDA ARS, Natl Sedimentat Lab, Watershed Phys Proc Res Unit, Oxford, MS 38655 USA. [Fox, G. A.] Oklahoma State Univ, Stillwater, OK 74078 USA. RP Wilson, GV (reprint author), USDA ARS, Natl Sedimentat Lab, Watershed Phys Proc Res Unit, Oxford, MS 38655 USA. EM glenn.wilson@ars.usda.gov NR 26 TC 4 Z9 4 U1 1 U2 17 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1168 EP 1181 DI 10.2136/sssaj2012.0416 PG 14 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200006 ER PT J AU Halvorson, JJ Gonzalez, JM Hagerman, AE AF Halvorson, Jonathan J. Gonzalez, Javier M. Hagerman, Ann E. TI Retention of Tannin-C is Associated with Decreased Soluble Nitrogen and Increased Cation Exchange Capacity in a Broad Range of Soils SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID POLYPHENOL-PROTEIN COMPLEXES; PLANT PHENOLIC-COMPOUNDS; ORGANIC-MATTER; CONDENSED TANNINS; ECOSYSTEM SERVICES; MANURE CHEMISTRY; FOOD-PRODUCTION; FOREST SOILS; LEAF-LITTER; CARBON AB Phenolic compounds, called tannins, can be retained by soil and affect soluble N but have been studied in only a few soil types. Surface samples (0-10 cm), collected from the United States and Canada, were treated with water (Control) or solutions containing procyanidin, catechin, beta-1,2,3,4,6-pentagalloyl-O-D-glucose (PGG), tannic acid, gallic acid, or methyl gallate. Soluble C and N in treatment supernatants and after incubation (16 h, 80 degrees C) were measured to determine retention of treatment C and effects on soluble N and cation exchange capacity (CEC). Retention varied significantly with treatment (T) and soil order (S) and was greatest for PGG > tannic acid > procyanidin > catechin > methyl gallate > gallic acid and in Alfisols, Aridisols and Mollisols compared Ultisols. However, differences among soil orders were observed only for strongly retained compounds (T x S, P < 0.001). Extraction of soluble N was decreased by gallic acid and tannins, especially PGG, but unaffected by methyl gallate or catechin. All treatments decreased soluble N from Aridisols while Entisols were less affected by tannins (T x S, P < 0.01). Soil CEC was significantly increased by tannins but unaffected by other compounds. However, CEC increased more in Aridisols than in Mollisols or Ultisols and treatment effects were small and unvarying in Ultisols (T x S, P < 0.001). Changes to both soluble N and CEC were linearly related with retention of treatment C. Tannins produced effects associated with improved soil quality on a broad range of soils and may have a role in land management. C1 [Halvorson, Jonathan J.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Gonzalez, Javier M.] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. [Hagerman, Ann E.] Miami Univ, Dept Chem & Biochem, Oxford, OH 45056 USA. RP Halvorson, JJ (reprint author), USDA ARS, No Great Plains Res Lab, POB 459, Mandan, ND 58554 USA. EM Jonathan.Halvorson@ars.usda.gov NR 72 TC 2 Z9 2 U1 2 U2 27 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1199 EP 1213 DI 10.2136/sssaj2012.0326 PG 15 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200009 ER PT J AU Sainju, UM Stevens, WB Evans, RG Iversen, WM AF Sainju, Upendra M. Stevens, William B. Evans, Robert G. Iversen, William M. TI Irrigation System and Tillage Effects on Soil Carbon and Nitrogen Fractions SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID SUGAR-BEET; MICROBIAL BIOMASS; ORGANIC-MATTER; COVER CROPS; SEQUENCE; SORGHUM; STORAGE; NITRATE; MINERALIZATION; FERTILIZATION AB Irrigation and tillage systems may affect surface residue and soil C and N fractions by influencing crop biomass yield, residue placement, and movement of water soluble C and N in the soil. We studied the effects of irrigation (mid-elevation spray application [MESA] and low energy precision application [LEPA]) and tillage (conventional [CT] and strip-tillage [ST]) systems on crop biomass (stems and leaves) yield, surface residue, and soil C and N fractions at the 0- to 20-cm depth from 2004 to 2007 in a Savage clay loam (fine, smectitic, frigid Vertic Argiustolls) in Sidney, MT. Soil C and N fractions were soil organic carbon (SOC) and total nitrogen (STN), particulate organic carbon and nitrogen (POC and PON), microbial biomass carbon and nitrogen (MBC and MBN), potential carbon and nitrogen mineralization (PCM and PNM), NH4-N, and NO3-N. While crop biomass across treatments increased from 2004 to 2007, surface residue was greater with ST than with CT from 2005 to 2007. The NH4-N and NO3-N contents at 5 to 10 and 10 to 20 cm in 2005 and STN at 0 to 5 cm in 2007 were greater with ST than with CT, but SOC at 5 to 10 and 10 to 20 cm, POC and MBN at 5 to 10 cm, and PNM at 0 to 5 cm in 2007 were greater with CT than with ST. The MBC at 0 to 5 cm and MBN at 10 to 20 cm were greater in LEPA than in MESA. The PCM at 10 to 20 cm was greater with CT than with ST in LEPA. While ST increased surface soil residue and N storage, residue incorporation to a greater depth in CT increased soil C storage, microbial activity, and N mineralization. Slow rate of water application near the soil surface increased microbial biomass in LEPA. C1 [Sainju, Upendra M.; Stevens, William B.; Evans, Robert G.; Iversen, William M.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Sainju, UM (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. EM Upendra.sainju@ars.usda.gov NR 32 TC 5 Z9 6 U1 3 U2 31 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1225 EP 1234 DI 10.2136/sssaj2012.0412 PG 10 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200011 ER PT J AU Wilson, MA Indorante, SJ Lee, BD Follmer, LR Williams, DR Fitch, BC McCauley, WM Bathgate, JD Kabrick, JM AF Wilson, M. A. Indorante, S. J. Lee, B. D. Follmer, L. R. Williams, D. R. Fitch, B. C. McCauley, W. M. Bathgate, J. D. Kabrick, J. M. TI Soil Geochemistry of Loess Landscapes, Shawnee Hills, Southern Illinois, United States SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID ORGANIC-MATTER; SPATIAL-DISTRIBUTION; INTENSE RAINFALL; MANGANESE OXIDES; SURFACE SOILS; HEAVY-METALS; TRACE-METALS; POWER-PLANT; MERCURY; WETLAND AB A study was initiated in soils of two southern Illinois loess-derived landscapes to investigate the distribution of selected trace and major elements. The objective of the research was to link the distribution of elements in different landscape positions to important soil and landscape processes. Seventeen pedons (representing different geomorphic hillslope positions) at the Morgan Pond (MP) and Bean Ridge (BR) sites were sampled by horizon. Data were evaluated by grouping selected horizons (A, Bt) by site (MP and BR) as well as by geomorphic hillslope position. Results show that relative concentrations of total As, Cr, Cu, V, Ni, Pb, Se, and Zn between these two sites were impacted by differences in inherent particle size distribution of the parent material. These elements were also greater in Bt versus A horizons within each site, suggesting that select elements were redistributed by clay illuviation processes. Barium, Ca, Cd, Hg, and Pb were all significantly elevated in A relative to Bt horizons at the BR site and were strongly correlated with total C, suggesting biocycling and trace element bioaccumulation were important in landscape positions of this site. The MP site lacked this same concentration of elements in surface horizons, demonstrating the effect of accelerated erosion due to more intensive cropping history. When grouping data by landscape position, elemental distribution and Bt/A clay ratios showed that landscape stability was greatest in summit positions relative to other positions (summit > shoulder > backslope positions), confirming that relative amount of soil erosion was also related to landscape position among these sites. C1 [Wilson, M. A.] USDA NRCS, Lincoln, NE 68508 USA. [Indorante, S. J.; Williams, D. R.; Fitch, B. C.; Bathgate, J. D.] USDA NRCS, MLRA Soil Survey Off, Carbondale, IL 62903 USA. [Lee, B. D.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Follmer, L. R.] Illinois State Geol Survey, Champaign, IL 61820 USA. [McCauley, W. M.] USDA NRCS, MLRA Soil Survey Off, Owensboro Serv Ctr, Owensboro, KY 42303 USA. [Kabrick, J. M.] Univ Missouri, US Forest Serv, USDA, No Res Stn, Columbia, MO 65211 USA. RP Wilson, MA (reprint author), USDA NRCS, 100 Centennial Mall N, Lincoln, NE 68508 USA. EM mike.wilson@lin.usda.gov NR 97 TC 2 Z9 2 U1 3 U2 32 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1269 EP 1283 DI 10.2136/sssaj2013.02.0051 PG 15 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200015 ER PT J AU Langner, AN Manu, A Nath, DA AF Langner, Amy N. Manu, Andrew Nath, Dan A. TI Dynamic Soil Properties across a Suburban Landscape SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID BULK-DENSITY; URBAN SOILS; CARBON AB Soil properties in urban settings are impacted by human activities. This study investigated human influence on dynamic soil properties including bulk density (BD), organic C (OC), pH, and inorganic C (IC) in the suburban environment of Ankeny, IA. Nine periods of urban development were delineated, and 10 homes from each period were randomly selected. Soils were sampled from front yards of these homes. All soils had a loamy texture. Mean BD values were significantly higher (1.70 g cm(-3)) in newer developments than older sites (1.23 g cm(-3)). Bulk density increased significantly with depth. This could be the effect of increased compaction associated with newer and heavier equipment in the case of newer developments and improved aggregation and structural development in soils in older areas. Soils from the 2003 to 2005 period had significantly lower levels of OC (0.49 g kg(-1)) than those from the pre(-1)939 period (4.49 g kg(-1)). Mean OC decreased with depth. The pH ranged from 5.4 to 8.5, with a mean of 7.7, and pH increased with time of development and with depth. Disturbance at newer sites could have exposed calcareous till. Long-term use of acid fertilizers could have decreased surface pH. The amount of IC ranged from 0.11 g kg(-1) in the older development to 8.41 g kg(-1) in the newest development. The IC increased with depth. These results could indicate that time has impacted the loss of IC through leaching and acid-forming processes. We conclude that current intensive site preparation techniques significantly affect dynamic soil properties; however, this impact is reduced as the time since the initial disturbance increases. C1 [Langner, Amy N.] USDA NRCS, Tech Soil Serv, Marcy, NY 13403 USA. [Manu, Andrew] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Nath, Dan A.] USDA NRCS, Soil Survey Div, Albert Lea, MN 56007 USA. RP Manu, A (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM akmanu@iastate.edu NR 39 TC 1 Z9 1 U1 5 U2 29 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1284 EP 1293 DI 10.2136/sssaj2012.0239 PG 10 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200016 ER PT J AU Shipitalo, MJ Owens, LB Bonta, JV Edwards, WM AF Shipitalo, Martin J. Owens, Lloyd B. Bonta, James V. Edwards, William M. TI Effect of No-Till and Extended Rotation on Nutrient Losses in Surface Runoff SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID INPUT CROPPING SYSTEMS; CORN-SOYBEAN ROTATION; HERBICIDE LOSSES; PHOSPHORUS; SOIL; OHIO; PERFORMANCE; WATERSHEDS; EROSION AB No-till crop production can reduce soil erosion compared to conventional tillage, but there is concern that lack of fertilizer incorporation will increase loss of dissolved nutrients in surface runoff. Therefore, we compared nutrient and sediment loss from two no-till and two chisel-till small (0.45-0.79 ha) watersheds managed in a 2-yr corn (Zea mays L.)/soybean [ Glycine max (L.) Merr]-rye (Secale cereale L.) cover crop rotation for 16 crop years. We also investigated the effect of replacing some of the mineral N with manure and red clover by monitoring runoff from three watersheds in a 3-yr corn/soybean/wheat-red clover (Triticum aestivum L.-Trifolium pratense L.) extended rotation. The reduced-input watersheds were disked in corn and soybean years. For all tillage treatments, NO3-N losses were greatest with corn and averaged from 3.7 (no-till) to 5.5 kg ha(-1) yr(-1) (chisel) for all crop years. Average dissolved P losses were 0.21 kg ha(-1) yr(-1) (chisel), 0.32 kg ha(-1) yr(-1) (no-till), and 0.84 kg ha(-1) yr(-1) (reduced-input). Total P losses were least from no-till (0.79 kg ha(-1) yr(-1)), intermediate for chisel (0.84 kg ha(-1) yr(-1)), and greatest for reduced-input watersheds (1.68 kg ha(-1) yr(-1)). Soil loss was least from no-till (807 kg ha(-1) yr(-1)), intermediate from chisel (1073 kg ha(-1) yr(-1)), and greatest from reduced-input watersheds (1177 kg ha(-1) yr(-1)). Thus, no-till had only slightly greater dissolved P losses compared to chisel, but had smaller soil, NO3-N, and total P losses. The greater dissolved and total P losses, as well as greater soil loss, compared to chisel and no-till watersheds, indicated that there was no surface water quality benefit to the reduced-input extended rotation. C1 [Shipitalo, Martin J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Owens, Lloyd B.; Edwards, William M.] USDA ARS, Washington, DC 20250 USA. [Bonta, James V.] USDA ARS, Oxford, MS 38655 USA. RP Shipitalo, MJ (reprint author), USDA ARS, Natl Lab Agr & Environm, 2110 Univ Blvd, Ames, IA 50011 USA. EM martin.shipitalo@ars.usda.gov OI Shipitalo, Martin/0000-0003-4775-7345 NR 32 TC 10 Z9 10 U1 2 U2 61 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1329 EP 1337 DI 10.2136/sssaj2013.01.0045 PG 9 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200020 ER PT J AU Dalzell, BJ Johnson, JMF Tallaksen, J Allan, DL Barbour, NW AF Dalzell, Brent J. Johnson, Jane M. F. Tallaksen, Joel Allan, Deborah L. Barbour, Nancy W. TI Simulated Impacts of Crop Residue Removal and Tillage on Soil Organic Matter Maintenance SO SOIL SCIENCE SOCIETY OF AMERICA JOURNAL LA English DT Article ID CARBON SEQUESTRATION; CONTINUOUS CORN; NITROGEN; MANAGEMENT; ROTATION; CQESTR; STOCKS; MODEL; DECOMPOSITION; SWITCHGRASS AB Cellulosic biofuel production may generate new markets and revenue for farmers. However, residue removal may cause environmental problems such as soil erosion and soil organic matter (SOM) loss. The objective of this study was to determine the amounts of residue necessary for SOM maintenance under different tillage and residue removal scenarios for corn-soybean [ Zea mays L.-Glycine max (L.) Merr.] and continuous corn rotations for a site in west-central Minnesota. We employed a process-based model (CQESTR) to evaluate management practices and quantify SOM changes over time. Results showed that conventional tillage resulted in SOM loss regardless of the amount of residue returned. Under no-till, residue amount was important in determining SOM accumulation or depletion. For the upper 30 cm of soil, average annual rates of 3.65 and 2.25 Mg crop residue ha(-1) yr(-1) were sufficient to maintain SOM for corn-soybean and continuous corn rotations, respectively. Soil OM in soil layers below 30 cm was predicted to decrease in all scenarios as a result of low root inputs. When considered over the upper 60 cm (maximum soil depth sampled), only continuous corn with no-till was sufficient to maintain SOM. Results from this work are important because they show that, for these management scenarios, no-till management is necessary for SOM maintenance and that determining whether SOM is accumulating or declining depends on the soil depth considered. At current yields observed in this study area, only continuous corn with no-till may generate enough residue to maintain or increase SOM. C1 [Dalzell, Brent J.; Allan, Deborah L.] Univ Minnesota Twin Cities, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Johnson, Jane M. F.; Barbour, Nancy W.] ARS, USDA, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Tallaksen, Joel] Univ Minnesota, West Cent Res & Outreach Ctr, Morris, MN 56267 USA. RP Dalzell, BJ (reprint author), Univ Minnesota Twin Cities, Dept Soil Water & Climate, Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. EM bdalzell@umn.edu OI Johnson, Jane/0000-0002-1687-4007 FU Xcel Energy through Renewable Development Fund; USDA-Agricultural Research Service; North Central Regional SunGrant Center at South Dakota State University through USDOE- Office of Biomass Programs [DE-FC36-05GO85041] FX We thank Alan Olness (ARS retired) and Jana Rinke for 1995 to 2004 plot records and field management notes. Funding for this project provide by customers of Xcel Energy through a grant from the Renewable Development Fund, with additional funding provided by the USDA-Agricultural Research Service, and from the North Central Regional SunGrant Center at South Dakota State University through a grant provided by the USDOE- Office of Biomass Programs under award number DE-FC36-05GO85041. NR 42 TC 7 Z9 7 U1 2 U2 39 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 0361-5995 J9 SOIL SCI SOC AM J JI Soil Sci. Soc. Am. J. PD JUL-AUG PY 2013 VL 77 IS 4 BP 1349 EP 1356 DI 10.2136/sssaj2012.0221 PG 8 WC Soil Science SC Agriculture GA 186YZ UT WOS:000322083200022 ER PT J AU Yee, WL Chapman, PS Sheets, HD AF Yee, Wee L. Chapman, Peter S. Sheets, H. David TI Comparative Body Size and Shape Analyses of F-1 Hybrid Rhagoletis pomonella and Rhagoletis zephyria (Diptera: Tephritidae) SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE apple maggot fly; hybridization; geometric morphometrics; wing shape; surstylus shape ID APPLE MAGGOT FLY; DISCRIMINANT-ANALYSIS; CENTRAL WASHINGTON; WING SHAPE; HYBRIDIZATION; MORPHOLOGY; MORPHOMETRICS; VARIABILITY; POPULATIONS; SPECIATION AB Hybridization between apple maggot, Rhagoletis pomonella (Walsh), and Rhagoletis zephyria Snow (Diptera: Tephritidae) occurs in Washington State, complicating fly identifications. Here, experimentally generated F 1 hybrids of R. pomonella and R. zephyria were classified using morphometric methods. Five of nine mean body size measurements of hybrids from crossing female R. pomonella x male R. zephyria were intermediate between those of nonhybrids. Aculeus lengths of hybrid females were intermediate or were more similar to those of R. pomonella than R. zephyria. Based on six or seven body size measures, 25.0% of female and 44.4% of male hybrids were correctly classified. Misclassified female and male hybrids resembled R. pomonella and one or the other parental species, respectively. Mean wing shapes of hybrid females based on landmark measurements were novel, whereas those of males were intermediate between those of nonhybrids. Based on wing shapes + natural log (ln) centroid size, 90.9% of female and 66.7% of male hybrids were correctly classified. Hybrid females were misclassified as R. pomonella and more hybrid males as R. zephyria. Based on surstylus shape, 27.7% of male hybrids were correctly classified, with more being misclassified as R. pomonella. Results show wing shape + ln centroid size is the most useful measure for identifying female and male hybrids and that R. pomonella traits for aculeus length and/or wing shape appear dominant to those of R. zephyria in hybrid females. C1 [Yee, Wee L.; Chapman, Peter S.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Sheets, H. David] Canisius Coll, Dept Phys, Buffalo, NY 14208 USA. RP Yee, WL (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov FU USDA Foreign Agricultural Service FX We thank Robert Goughnour (Washington State University, Vancouver) for collecting infested fruit and for generating the hybrid flies, Janine Jewett and Dana Jones (USDAD-ARS, Wapato, WA) for laboratory assistance, and Ed Lisowski (Washington State Department of Agriculture, Yakima, WA), and Eric LaGasa (Washington State Department of Agriculture, Olympia, WA) for reviewing earlier drafts of the manuscript. This work was partially supported by a grant from the USDA Foreign Agricultural Service to W.L.Y. NR 43 TC 1 Z9 1 U1 1 U2 9 PU ENTOMOLOGICAL SOC AMER PI LANHAM PA 10001 DEREKWOOD LANE, STE 100, LANHAM, MD 20706-4876 USA SN 0013-8746 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2013 VL 106 IS 4 BP 410 EP 423 DI 10.1603/AN13016 PG 14 WC Entomology SC Entomology GA 182VK UT WOS:000321772700003 ER PT J AU Backus, EA Rangasamy, M Stamm, M Mcauslane, HJ Cherry, R AF Backus, Elaine A. Rangasamy, Murugesan Stamm, Mitchell Mcauslane, Heather J. Cherry, Ron TI Waveform Library for Chinch Bugs (Hemiptera: Heteroptera: Blissidae): Characterization of Electrical Penetration Graph Waveforms at Multiple Input Impedances SO ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA LA English DT Article DE Insecta; Blissus; EPG; feeding; stylet penetration ID BLISSUS-OCCIDUUS HEMIPTERA; STYLET PENETRATION; XYLELLA-FASTIDIOSA; ST-AUGUSTINEGRASS; HESPERUS HEMIPTERA; PROBING BEHAVIOR; LYGAEIDAE; RESISTANCE; BUFFALOGRASS; CICADELLIDAE AB Electrical penetration graph (EPG) monitoring has been used extensively to elucidate mechanisms of resistance in plants to insect herbivores with piercing-sucking mouthparts. Characterization of waveforms produced by insects during stylet probing is essential to the application of this technology. In the studies described herein, a four-channel Backus and Bennett AC-DC monitor was used to characterize EPG waveforms produced by adults of two economically important chinch bug species: southern chinch bug, Blissus insularis Barber, feeding on St. Augustinegrass, and western chinch bug, Blissus occiduus Barber, feeding on buffalograss. This is only the third time a heteropteran species has been recorded by using EPG; it is also the first recording of adult heteropterans, and the first of Blissidae. Probing of chinch bugs was recorded with either AC or DC applied voltage, no applied voltage, or voltage switched between AC and DC mid-recording, at input impedances ranging from 10(6) to 10(10)Omega, plus 10(13)Omega, to develop a waveform library. Waveforms exhibited by western and southern chinch bugs were similar, and both showed long periods of putative pathway and ingestion phases (typical of salivary sheath feeders) interspersed with shorter phases, termed transitional J wave and interruption. The J wave is suspected to be an X wave, that is, in EPG parlance, a stereotypical transition waveform that marks contact with a preferred ingestion tissue. The flexibility of using multiple input impedances with the AC-DC monitor was valuable for determining the electrical origin (resistance vs. electromotive force components) of the chinch bug waveforms. It was concluded that an input impedance of 10(7) Omega, with either DC or AC applied voltage, is optimal to detect all resistance-and electromotive force-component waveforms produced during chinch bug probing. Knowledge of electrical origins suggested hypothesized biological meanings of the waveforms, before time-intensive future correlation experiments by using histology, microscopy, and other techniques. C1 [Backus, Elaine A.] ARS, Crop Dis Pests & Genet Res Unit, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Rangasamy, Murugesan; Mcauslane, Heather J.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Stamm, Mitchell] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Cherry, Ron] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. RP Backus, EA (reprint author), ARS, Crop Dis Pests & Genet Res Unit, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM elaine.backus@ars.usda.gov FU Wedgworth fellowship; Florida Agricultural Experiment Station FX We thank Holly Shugart and Jose Gutierrez (U.S. Department of Agriculture-Agriculture Research Service) for their help during the EPG Workshop at California State University, Fresno, in 2005, and for numerous subsequent attempts to collect excretory droplets and perform plant histological correlations for southern chinch bug waveforms. We thank Russell Nagata (UF/IFAS, Everglades Research and Education Center) for supplying St. Augustinegrass. This research was supported by a Wedgworth fellowship (to M.R.) and the Florida Agricultural Experiment Station. NR 41 TC 6 Z9 6 U1 1 U2 13 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0013-8746 EI 1938-2901 J9 ANN ENTOMOL SOC AM JI Ann. Entomol. Soc. Am. PD JUL PY 2013 VL 106 IS 4 BP 524 EP 539 DI 10.1603/AN13015 PG 16 WC Entomology SC Entomology GA 182VK UT WOS:000321772700017 ER PT J AU Lee, FS Pham, X Gu, G AF Lee, Frederic S. Xuan Pham Gu, Gyun TI The UK Research Assessment Exercise and the narrowing of UK economics SO CAMBRIDGE JOURNAL OF ECONOMICS LA English DT Article DE UK economics; Research Assessment Exercise; A14; B5; A11 ID JOURNALS; RANKING; UNIVERSITIES AB The aim of this article is to delineate an empirically grounded, structure-causal going concern recursive model of UK economics that, in the context of the Research Assessment Exercise (RAE) and local department decision making, explains the progressive elimination of heterodox economics, the progressive homogenisation of mainstream economics from 1992 to the present and the continued rise to dominance of a select group of departments, and indicates whether these events will continue under the Research Excellence Framework selectivity exercise in 2014. C1 [Lee, Frederic S.] Univ Missouri, Kansas City, MO 65110 USA. [Xuan Pham] USDA, Washington, DC USA. RP Lee, FS (reprint author), Univ Missouri, Dept Econ, 5100 Rockhill Rd, Kansas City, MO 65110 USA. EM leefs@umkc.edu NR 40 TC 19 Z9 19 U1 1 U2 16 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0309-166X EI 1464-3545 J9 CAMB J ECON JI Cambr. J. Econ. PD JUL PY 2013 VL 37 IS 4 BP 693 EP 717 DI 10.1093/cje/bet031 PG 25 WC Economics SC Business & Economics GA 182II UT WOS:000321734500001 ER PT J AU Bridges, GA Day, ML Geary, TW Cruppe, LH AF Bridges, G. A. Day, M. L. Geary, T. W. Cruppe, L. H. TI TRIENNIAL REPRODUCTION SYMPOSIUM: Deficiencies in the uterine environment and failure to support embryonic development SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; embryonic loss; estradiol; progesterone; uterus ID LACTATING DAIRY-COWS; PREOVULATORY ESTRADIOL CONCENTRATIONS; MILK PROGESTERONE CONCENTRATION; GONADOTROPIN-RELEASING-HORMONE; TIMED ARTIFICIAL-INSEMINATION; INDUCED TRANSCRIPTOME CHANGES; MESSENGER-RNA EXPRESSION; EARLY LUTEAL-PHASE; SUCKLED BEEF-COWS; ESTROUS-CYCLE AB Pregnancy failure in livestock can result from failure to fertilize the oocyte or embryonic loss during gestation. The focus of this review is on cattle and factors affecting and mechanisms related to uterine insufficiency for pregnancy. A variety of factors contribute to embryonic loss and it may be exacerbated in certain animals, such as high-producing lactating dairy cows, and in some cattle in which estrous synchronization and timed AI was performed, due to reduced concentrations of reproductive steroids. Recent research in beef cattle induced to ovulate immature follicles and in lactating dairy cows indicates that deficient uterine function is a major factor responsible for infertility in these animals. Failure to provide adequate concentrations of estradiol before ovulation results in prolonged effects on expression and localization of uterine genes and proteins that participate in regulating uterine functions during early gestation. Furthermore, progesterone concentrations during early gestation affect embryonic growth, interferontau production, and uterine function. Therefore, an inadequate uterine environment induced by insufficient steroid concentrations before and after ovulation could cause early embryonic death either by failing to provide an adequate uterine environment for recognition of embryo signaling, adhesion, and implantation or by failing to support appropriate embryonic growth, which could lead to decreased conceptus size and failed maternal recognition of pregnancy. C1 [Bridges, G. A.] Univ Minnesota, North Cent Res & Outreach Ctr, Grand Rapids, MI 55744 USA. [Day, M. L.; Cruppe, L. H.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. [Geary, T. W.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Bridges, GA (reprint author), Univ Minnesota, North Cent Res & Outreach Ctr, Grand Rapids, MI 55744 USA. EM gbridges@umn.edu RI Day, Michael/K-4311-2013 FU James Lauderdale Appreciation Club; ASAS Foundation, Elanco Animal Health (Greenfield, IN); Pfizer Animal Health (New York, NY); American Society of Animal Science; Journal of Animal Science FX Based on a presentation at the Triennial Reproduction Symposium titled "Impediments to Fertility in Domestic Animals" preceding the Joint Annual Meeting, July 15-19, 2012, Phoenix, Arizona. The symposium was sponsored, in part, by the The James Lauderdale Appreciation Club, The ASAS Foundation, Elanco Animal Health (Greenfield, IN), and Pfizer Animal Health (New York, NY), with publication sponsored by the American Society of Animal Science and the Journal of Animal Science. NR 108 TC 27 Z9 28 U1 0 U2 32 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3002 EP 3013 DI 10.2527/jas.2013-5882 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200003 PM 23798511 ER PT J AU Geary, TW Smith, MF MacNeil, MD Day, ML Bridges, GA Perry, GA Abreu, FM Atkins, JA Pohler, KG Jinks, EM Madsen, CA AF Geary, T. W. Smith, M. F. MacNeil, M. D. Day, M. L. Bridges, G. A. Perry, G. A. Abreu, F. M. Atkins, J. A. Pohler, K. G. Jinks, E. M. Madsen, C. A. TI TRIENNIAL REPRODUCTION SYMPOSIUM: Influence of follicular characteristics at ovulation on early embryonic survival SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; embryonic survival; ovary; pregnancy ID GONADOTROPIN-RELEASING-HORMONE; IN-VITRO MATURATION; LACTATING DAIRY-COWS; BOVINE ESTROUS-CYCLE; SUCKLED BEEF-COWS; BOS-INDICUS COWS; LUTEINIZING-HORMONE; DEVELOPMENTAL COMPETENCE; TRANSCRIPTIONAL ACTIVITY; PREGNANCY ESTABLISHMENT AB Reproductive failure in livestock can result from failure to fertilize the oocyte or embryonic loss during gestation. Although fertilization failure occurs, embryonic mortality represents a greater contribution to reproductive failure. Reproductive success varies among species and production goals but is measured as a binomial trait (i.e., pregnancy), derived by the success or failure of multiple biological steps. This review focuses primarily on follicular characteristics affecting oocyte quality, fertilization, and embryonic health that lead to pregnancy establishment in beef cattle. When estrous cycles are manipulated with assisted reproductive technologies and ovulation is induced, duration of proestrus (i.e., interval from induced luteolysis to induced ovulation), ovulatory follicle growth rate, and ovulatory follicle size are factors that affect the maturation of the follicle and oocyte at induced ovulation. The most critical maturational component of the ovulatory follicle is the production of sufficient estradiol to prepare follicular cells for luteinization and progesterone synthesis and prepare the uterus for pregnancy. The exact roles of estradiol in oocyte maturation remain unclear, but cows that have lesser serum concentrations of estradiol have decreased fertilization rates and decreased embryo survival on d 7 after induced ovulation. When length of proestrus is held constant, perhaps the most practical follicular measure of fertility is ovulatory follicle size because it is an easily measured attribute of the follicle that is highly associated with its ability to produce estradiol. C1 [Geary, T. W.; MacNeil, M. D.; Madsen, C. A.] USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Smith, M. F.; Atkins, J. A.; Pohler, K. G.; Jinks, E. M.] Univ Missouri, Div Anim Sci, Columbia, MO 65211 USA. [Day, M. L.; Abreu, F. M.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. [Bridges, G. A.] Univ Minnesota, North Cent Res & Outreach Ctr, Grand Rapids, MI 55744 USA. [Perry, G. A.] S Dakota State Univ, Dept Anim Sci, Brookings, SD 57007 USA. RP Geary, TW (reprint author), USDA ARS, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. EM tom.geary@ars.usda.gov RI Day, Michael/K-4311-2013 FU James Lauderdale Appreciation Club; ASAS Foundation, Elanco Animal Health (Greenfield, IN); Pfizer Animal Health (New York, NY); American Society of Animal Science; Journal of Animal Science FX Based on a presentation at the Triennial Reproduction Symposium titled "Impediments to Fertility in Domestic Animals" preceding the Joint Annual Meeting, July 15-19, 2012, Phoenix, Arizona. The symposium was sponsored, in part, by the The James Lauderdale Appreciation Club, The ASAS Foundation, Elanco Animal Health (Greenfield, IN), and Pfizer Animal Health (New York, NY), with publication sponsored by the American Society of Animal Science and the Journal of Animal Science. NR 86 TC 19 Z9 19 U1 1 U2 14 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3014 EP 3021 DI 10.2527/jas.2012-5887 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200004 PM 23230106 ER PT J AU Vallet, JL McNeel, AK Johnson, G Bazer, FW AF Vallet, J. L. McNeel, A. K. Johnson, G. Bazer, F. W. TI TRIENNIAL REPRODUCTION SYMPOSIUM: Limitations in uterine and conceptus physiology that lead to fetal losses SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE erythropoiesis; hyaluronan; placenta ID OVULATION RATE; LITTER SIZE; INTRAUTERINE MIGRATION; HEPARAN-SULFATE; ENDOMETRIAL DEVELOPMENT; TRANSUTERINE MIGRATION; PLACENTAL EFFICIENCY; GLUCOSE TRANSPORTERS; PREGNANCY SUCCESS; PORCINE EMBRYO AB Conceptus losses in livestock occur throughout gestation. The uterus and the embryo-placenta-fetus play interconnected roles in these losses, the details of which depend on the period of gestation and the species. Studies in sheep and pigs have indicated that the uterine glands are essential for full fertility, based on experiments where gland development was reduced through the use of exogenous hormones. In sheep and cattle, normally the uterus is well able to support more than a single fetus although these species differ in the consequences of multiple births. When 2 conceptuses are present, the placentas of cattle often anastomose, putting 1 fetus at risk if the other is lost. One likely reason this does not occur in sheep is because sheep embryos undergo intrauterine migration, similar to pigs. In pigs, the relatively equidistant separation of conceptuses is likely to be essential for optimizing conceptus survival as is the simultaneous and uniform elongation of blastocysts that occurs during the time of maternal recognition of pregnancy. Other studies in pigs have indicated that the size of the uterus influences litter size and therefore fetal losses. In response to crowded intrauterine conditions in the pig, increased conceptus losses begin to occur between d 30 and 40 of pregnancy, and further losses occur sporadically during later gestation. There is evidence that improved fetal erythropoiesis can reduce these losses. Other studies indicated that profound changes in placental development occurred under crowded intrauterine conditions that may contribute to losses during late gestation. Reductions in placental stroma formation may compromise the ability of the pig placenta to adapt to reduced uterine space. Consistent with this, both hyaluronan and hyaluronidase activity are decreased in the placentas of small compared with large fetuses. These results indicate that improvements in placental stroma formation could improve placental ability to compensate for reduced intrauterine space, resulting in increased placental function and reduced fetal losses during late gestation. C1 [Vallet, J. L.; McNeel, A. K.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Johnson, G.] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA. [Bazer, F. W.] USDA ARS, Dept Anim Sci, Clay Ctr, NE 68933 USA. RP Vallet, JL (reprint author), USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Jeff.Vallet@ars.usda.gov FU James Lauderdale Appreciation Club; ASAS Foundation, Elanco Animal Health (Greenfield, IN); Pfizer Animal Health (New York, NY); American Society of Animal Science; Journal of Animal Science FX Based on a presentation at the Triennial Reproduction Symposium titled "Impediments to Fertility in Domestic Animals" preceding the Joint Annual Meeting, July 15-19, 2012, Phoenix, Arizona. The symposium was sponsored, in part, by the The James Lauderdale Appreciation Club, The ASAS Foundation, Elanco Animal Health (Greenfield, IN), and Pfizer Animal Health (New York, NY), with publication sponsored by the American Society of Animal Science and the Journal of Animal Science. NR 100 TC 10 Z9 11 U1 2 U2 19 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3030 EP 3040 DI 10.2527/jas.2012-6138 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200006 PM 23798512 ER PT J AU Oliver, WT Wells, JE AF Oliver, W. T. Wells, J. E. TI Lysozyme as an alternative to antibiotics improves growth performance and small intestinal morphology in nursery pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE antimicrobial; growth; intestine; lysozyme; swine ID ESCHERICHIA-COLI K88; 10-DAY-OLD PIGS; MILK REPLACER; WEANED PIGS; TRANSPORT; SUPPLEMENTATION; LACTOFERRIN; MICROFLORA; PROMOTERS; SECRETION AB Lysozyme is a 1,4-beta-N-acetylmuramidase that has antimicrobial properties. The objective of this experiment was to determine if lysozyme in nursery diets improved growth performance and gastrointestinal health of pigs weaned from the sow at 24 d of age. Two replicates of 96 pigs (192 total; 96 males, 96 females) were weaned from the sow at 24 d of age, blocked by BW and gender, and then assigned to 1 of 24 pens (4 pigs/pen). Each block was randomly assigned 1 of 3 dietary treatments for 28 d:control (two 14-d phases), control + antibiotics (carbadox/copper sulfate), or control + lysozyme (100 mg/kg diet). Pigs were weighed and blood sampled on d 0, 14, and 28 of treatment. Blood was analyzed for plasma urea nitrogen (PUN) and IgA. At 28 d, pigs were killed, and samples of jejunum and ileum were collected and fixed for intestinal morphology measurements. An additional jejunum sample was taken from the 12 pigs with the median BW per treatment to determine transepithelial electrical resistance (TER). Pigs consuming antibiotics or lysozyme grew at a faster rate than control pigs (0.433 +/- 0.009 and 0.421 +/- 0.008 vs. 0.398 +/- 0.008 kg/d, respectively; P < 0.03), which resulted in heavier ending BW (20.00 +/- 0.31, 19.8 +/- 0.29, and 18.83 +/- 0.32 kg, respectively; P < 0.03). Feed intake was not different (P > 0.48), but G:F was improved in pigs consuming antibiotics or lysozyme (0.756 +/- 0.014, 0.750 +/- 0.021, and 0.695 +/- 0.019 kg/kg; P < 0.05). Immunoglobulin A (P < 0.03) and PUN (P < 0.01) increased during the experiment, regardless of dietary treatment (P > 0.48). Dietary treatment did not affect TER (P > 0.37), but gilts had lower TER compared with barrows (P < 0.04). No differences in villi height or crypt depth were observed in the ileum (P > 0.53). However, jejunum villi height was increased and crypt depth was decreased in pigs consuming antibiotics or lysozyme (P < 0.001), resulting in an increased villi height:crypt depth of 72% (P < 0.001). Thus, we concluded that lysozyme is a suitable alternative to carbadox/copper sulfate diets fed to pigs weaned from the sow at 24 d of age. C1 [Oliver, W. T.; Wells, J. E.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Oliver, WT (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM william.oliver@ars.usda.gov NR 40 TC 12 Z9 15 U1 3 U2 38 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3129 EP 3136 DI 10.2527/jas.2012-5782 PG 8 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200016 PM 23572262 ER PT J AU Lents, CA Rempel, LA Klindt, J Wise, T Nonneman, D Freking, BA AF Lents, C. A. Rempel, L. A. Klindt, J. Wise, T. Nonneman, D. Freking, B. A. TI The relationship of plasma urea nitrogen with growth traits and age at first estrus in gilts SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE estrus; gilt; growth; puberty; urea nitrogen ID BODY-COMPOSITION; REPRODUCTIVE-PERFORMANCE; BACKFAT THICKNESS; SELECTION; PUBERTY; WEIGHT; LEPTIN AB Gilts that reach puberty at an earlier age with more backfat have greater lifetime productivity. Increased growth rates generally promote earlier age at first estrus; however, an association of age at first estrus with discrete measures of body fatness remains controversial. We tested the hypothesis that metabolic state as determined by concentrations of plasma urea nitrogen (PUN), which reflect lean tissue growth, were correlated with age at first estrus. Blood samples were collected from gilts (n = 337) at 102, 123, and 145 d of age during development. Concentrations of albumin, creatinine, glucose, and PUN were determined. Body weight and backfat thickness were determined at each time point. From 130 to 240 d of age, gilts were monitored for first pubertal estrus. Concentrations of creatinine increased whereas concentrations of glucose decreased with increasing age (P < 0.0001). Concentrations of albumin and PUN remained relatively stable throughout development. Average daily BW gain (r = 0.22) and change in backfat thickness (r = 0.29) had a positive phenotypic correlation (P < 0.0001) with PUN at 145 d of age. Concentrations of PUN at 102 and 123 d of age were not phenotypically correlated with pubertal age, but there was a moderately negative phenotypic correlation (r = -0.22; P < 0.0001) of PUN at 145 d of age with age at first estrus along with a negative genetic correlation (r = -0.42). The relationship of PUN with age at first estrus shifted from liner to quadratic with advancing age. These data demonstrate that near the age at which gilts are selected for entry into the breeding unit, those with greater PUN have increased BW and backfat thickness and display pubertal estrus earlier but that PUN does not account for additional variation in age at first estrus beyond growth rate or backfat. It is concluded that PUN can be used to select gilts with increased efficiency of nutrient use without negatively impacting pubertal development. C1 [Lents, C. A.; Rempel, L. A.; Klindt, J.; Wise, T.; Nonneman, D.; Freking, B. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Lents, CA (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM clay.lents@ars.usda.gov NR 23 TC 1 Z9 2 U1 1 U2 4 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3137 EP 3142 DI 10.2527/jas.2012-5778 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200017 PM 23572260 ER PT J AU Kemp, CM Oliver, WT Wheeler, TL Chishti, AH Koohmaraie, M AF Kemp, C. M. Oliver, W. T. Wheeler, T. L. Chishti, A. H. Koohmaraie, M. TI The effects of Capn1 gene inactivation on skeletal muscle growth, development, and atrophy, and the compensatory role of other proteolytic systems SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE body composition; muscle fiber type; proteases ID TYROSINE-PHOSPHATASE 1B; MU-CALPAIN; MESSENGER-RNA; FIBER TYPES; CROSS-TALK; CASPASE; PROTEASE; BOVINE; DIFFERENTIATION; DEGRADATION AB Myofibrillar protein turnover is a key component of muscle growth and degeneration, requiring proteolytic enzymes to degrade the skeletal muscle proteins. The objective of this study was to investigate the role of the calpain proteolytic system in muscle growth development using mu-calpain knockout (KO) mice in comparison with control wild-type (WT) mice, and evaluate the subsequent effects of silencing this gene on other proteolytic systems. No differences in muscle development between genotypes were observed during the early stages of growth due to the up regulation of other proteolytic systems. The KO mice showed significantly greater m-calpain protein abundance (P < 0.01) and activity (P < 0.001), and greater caspase 3/7 activity (P < 0.05). At 30 wk of age, KO mice showed increased protein: DNA (P < 0.05) and RNA: DNA ratios (P < 0.01), greater protein content (P < 0.01) at the expense of lipid deposition (P < 0.05), and an increase in size and number of fast-twitch glycolytic muscle fibers (P < 0.05), suggesting that KO mice exhibit an increased capacity to accumulate and maintain protein in their skeletal muscle. Also, expression of proteins associated with muscle regeneration (neural cell adhesion molecule and myoD) were both reduced in the mature KO mice (P < 0.05 and P < 0.01, respectively), indicating less muscle regeneration and, therefore, less muscle damage. These findings indicate the concerted action of proteolytic systems to ensure muscle protein homeostasis in vivo. Furthermore, these data contribute to the existing evidence of the importance of the calpain system's involvement in muscle growth, development, and atrophy. Collectively, these data suggest that there are opportunities to target the calpain system to promote the growth and/or restoration of skeletal muscle mass. C1 [Kemp, C. M.; Oliver, W. T.; Wheeler, T. L.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Chishti, A. H.] Tufts Univ, Sch Med, Dept Mol Physiol & Pharmacol, Boston, MA 02111 USA. [Koohmaraie, M.] IEH Labs & Consulting Grp, Lake Forest Pk, WA 98155 USA. [Koohmaraie, M.] King Saud Univ, Coll Food & Agr, Riyadh, Saudi Arabia. RP Oliver, WT (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM william.oliver@ars.usda.gov FU NHLBI NIH HHS [R01 HL089517, R01 HL095050] NR 43 TC 0 Z9 0 U1 0 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3155 EP 3167 DI 10.2527/jas.2012-5737 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200019 PM 23798514 ER PT J AU Kerr, BJ Dozier, WA Shurson, GC AF Kerr, B. J. Dozier, W. A., III Shurson, G. C. TI Effects of reduced-oil corn distillers dried grains with solubles composition on digestible and metabolizable energy value and prediction in growing pigs SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE corn-distillers grains with solubles; energy; energy prediction; ether extract; growing-finishing pigs ID GROWER-FINISHER PIGS; NUTRIENT DIGESTIBILITY; PARTICLE-SIZE; DIETARY FIBER; LIQUID-CHROMATOGRAPHY; CHEMICAL-ANALYSIS; FEEDING DIETS; WHEAT-FLOUR; ADULT SOWS; AMINO-ACID AB Two experiments were conducted to determine the DE and ME content of corn distillers dried grains with solubles (corn-DDGS) containing variable ether extract (EE) concentrations and to develop DE and ME prediction equations based on chemical composition. Ether extract content of corn-DDGS ranged from 4.88 to 10.88% (DM basis) among 4 corn-DDGS samples in Exp. 1 and from 8.56 to 13.23% (DM basis) among 11 corn-DDGS samples in Exp. 2. The difference in concentration of total dietary fiber (TDF) and NDF among the 4 corn-DDGS sources was 2.25 and 3.40 percentage units, respectively, in Exp. 1 but was greater among the 11 corn-DDGS sources evaluated in Exp. 2, where they differed by 6.46 and 15.18 percentage units, respectively. The range in CP and ash were from 28.97 to 31.19% and 5.37 to 6.14%, respectively, in Exp. 1 and from 27.69 to 32.93% and 4.32 to 5.31%, respectively, in Exp. 2. Gross energy content among corn-DDGS samples varied from 4,780 to 5,113 kcal/kg DM in Exp. 1 and from 4,897 to 5,167 kcal/kg DM in Exp. 2. In Exp. 1, the range in DE content was from 3,500 to 3,870 kcal/kg DM and ME content varied from 3,266 to 3,696 kcal/kg DM. There were no differences in ME:DE content among the 4 corn-DDGS sources in Exp. 1, but ME:GE content differed (P = 0.04) among sources (66.82 to 74.56%). In Exp. 2, the range in DE content among the 11 corn-DDGS sources was from 3,474 to 3,807 kcal/kg DM and ME content varied from 3,277 to 3,603 kcal/kg DM. However, there were no differences in DE:GE, ME:DE, or ME:GE among sources in Exp. 2. In Exp. 1, no ingredient physical or chemical measurement [bulk density (BD), particle size, GE, CP, starch, TDF, NDF, ADF, hemicellulose, EE, or ash)] was statistically significant at P <= 0.15 to predict DE or ME content in corn-DDGS. In Exp. 2, the best fit DE equation was DE (kcal/kg DM) = 1,601 - (54.48 x % TDF) + (0.69 x % GE) + (731.5 x BD) [R-2 = 0.91, SE = 41.25]. The best fit ME equation was ME (kcal/kg DM) = 4,558 + (52.26 x % EE) - (50.08 x % TDF) [R-2 = 0.85, SE = 48.74]. Apparent total tract digestibility of several nutritional components such as ADF, EE, and N were quite variable among corn-DDGS sources in both experiments. These results indicate that although EE may be a good predictor of GE content in corn-DDGS, it is not a primary factor for predicting DE or ME content. Measures of dietary fiber, such as ADF or TDF, are more important than EE in determining the DE or ME content of corn-DDGS for growing pigs. C1 [Kerr, B. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Dozier, W. A., III] Auburn Univ, Auburn, AL 38649 USA. [Shurson, G. C.] Univ Minnesota, St Paul, MN 55108 USA. RP Kerr, BJ (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. EM brian.kerr@ars.usda.gov FU Minnesota Corn Research and Promotion Council (Shakopee, MN); Agricultural Utilization Research Institute (Crookston, MN); POET Nutrition LLC (Sioux Falls, SD) FX This research was financially supported by the Minnesota Corn Research and Promotion Council (Shakopee, MN), Agricultural Utilization Research Institute (Crookston, MN), and POET Nutrition LLC (Sioux Falls, SD). Mention of a trade name, proprietary product, or specific equipment does not constitute a guarantee or warranty by the USDA, Auburn University or the University of Minnesota and does not imply approval to the exclusion of other products that may be suitable. The authors gratefully acknowledge the assistance of D. Hedges (University of Minnesota, St. Paul) and J. Cook (USDA-ARS, Ames) with sample collection and laboratory analysis. The USDA is an equal opportunity provider and employer. NR 43 TC 22 Z9 22 U1 0 U2 12 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3231 EP 3243 DI 10.2527/jas.2013-6252 PG 13 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200026 PM 23798517 ER PT J AU Hales, KE Freetly, HC Shackelford, SD King, DA AF Hales, K. E. Freetly, H. C. Shackelford, S. D. King, D. A. TI Effects of roughage concentration in dry-rolled corn-based diets containing wet distillers grains with solubles on performance and carcass characteristics of finishing beef steers SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE cattle; distillers grains; roughage level ID IN-VITRO FERMENTATION; STEAM-FLAKED CORN; FEEDLOT PERFORMANCE; QUALITY; HEIFERS; LEVEL AB Distillers grains and distillers solubles are by-products of grain fermentation used to produce ethanol and contain greater concentrations of NDF and ADF, compared with other grains and concentrates they replace in feedlot diets. Typical finishing diets in the United States contain 8.3% and 9.0% roughage. Therefore, it is plausible that the dietary concentration of roughage can be altered when distillers grains are included in feedlot diets. The effects of roughage concentration in dry-rolled, corn-based diets containing wet distillers grains with solubles (WDGS) were evaluated in steers (n = 128; initial BW = 339 kg), using Calan gates. Each diet was based on dry-rolled corn and contained 25% WDGS with coarsely ground alfalfa hay (AH), replacing corn at 2% (AH-2), 6% (AH-6), 10% (AH-10), and 14% (AH-14) of DM. Feed offered was recorded daily, orts were measured weekly, and BW was measured on d 0, 1, 35, 70, 105, 140, 174, and 175. After commercial harvest and chilling, carcasses were evaluated on-line with a beef carcass grading camera to assess marbling and yield grade traits. The data were analyzed using the Mixed Procedure of SAS, in which contrast statements were used to separate linear and quadratic effects of AH inclusion. Decreasing concentrations of AH in the finishing diet resulted in a tendency for a quadratic response (P = 0.07) in final BW, where BW increased from 2 to 6% AH inclusion but then decreased from 6 to 14% inclusion. Similarly, ADG from d 0 to end responded quadratically (P < 0.01), in which ADG increased from 2 to 6% yet subsequently decreased from 6 to 14% AH inclusion. Dry matter intake from d 0 to end increased linearly (P = 0.02) as AH inclusion increased in the diet, whereas G:F increased from 2 to 6% AH inclusion and then decreased linearly (P < 0.01) from 6 to 14% AH inclusion. Concentration of AH in the finishing diet did not affect HCW, marbling score, or the proportion of cattle grading USDA choice (P >= 0.18). However, dressing percent and LM area did respond in a quadratic manner (P < 0.02), in which they decreased from 2 to 10% AH inclusion and increased from 10 to 14% AH in the diet. Yield grade and adjusted 12th rib fat responded quadratically (P < 0.01), in which both increased from 2 to 6% AH inclusion and decreased from 6 to 14% inclusion. Analysis of responses of G:F and ADG on AH predict the apex at 3% and 7% for G:F and ADG, respectively, when fed in diets containing 25% WDGS. C1 [Hales, K. E.; Freetly, H. C.; Shackelford, S. D.; King, D. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Hales, KE (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Kristin.Hales@ars.usda.gov NR 19 TC 2 Z9 2 U1 0 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 J9 J ANIM SCI JI J. Anim. Sci. PD JUL PY 2013 VL 91 IS 7 BP 3315 EP 3321 DI 10.2527/jas2012-5942 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA 170MO UT WOS:000320856200035 PM 23798521 ER PT J AU De Lucca, AJ Carter-Wientjes, CH Boue, SM Lovisa, MP Bhatnagar, D AF De Lucca, Anthony J. Carter-Wientjes, Carol H. Boue, Stephen M. Lovisa, Mary P. Bhatnagar, Deepak TI Inhibition of Bacterial and Filamentous Fungal Growth in High Moisture, Nonsterile Corn with Intermittent Pumping of Trans-2-Hexenal Vapor SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antibacterial; antifungal; corn; fumigation; trans-2-hexenal ID ASPERGILLUS-FLAVUS GROWTH; ESSENTIAL OILS; FUSARIUM-VERTICILLIOIDES; ANTIMICROBIAL ACTIVITY; AFLATOXIN PRODUCTION; ACREMONIUM-ZEAE; MAIZE; CONTAMINATION; PATHOGENS; CINNAMON AB Trans-2-hexenal (T2H), a plant-produced aldehyde, was intermittently pumped over a 7 d period into a small, bench top model of stored corn (nonsterile, moisture content about 23%). Naturally occurring bacteria and fungi, including added Aspergillus flavus, grew rapidly on corn not treated with T2H vapor. However, intermittently pumped T2H (30 min per 2 h or 30 min per 12 h) significantly reduced bacterial and fungal viable populations, with nearly 100% fungal viability loss observed after either (1) one day of pumping at the 30 min per 2 h rate or (2) pumping cycles of 30 min per 12 h period over the initial 48 to 72 h of incubation. Data suggest that short-term intermittent fumigation of stored corn with T2H could prevent growth of bacteria and mycotoxigenic fungi such as A. flavus. C1 [De Lucca, Anthony J.; Carter-Wientjes, Carol H.; Boue, Stephen M.; Lovisa, Mary P.; Bhatnagar, Deepak] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP De Lucca, AJ (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM anthony.delucca@ars.usda.gov NR 34 TC 2 Z9 2 U1 1 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD JUL PY 2013 VL 78 IS 7 BP M1029 EP M1035 DI 10.1111/1750-3841.12151 PG 7 WC Food Science & Technology SC Food Science & Technology GA 185HN UT WOS:000321958400012 PM 23865451 ER PT J AU Mukhopadhyay, S Ukuku, D Fan, X Juneja, VK AF Mukhopadhyay, S. Ukuku, D. Fan, X. Juneja, V. K. TI Efficacy of Integrated Treatment of UV light and Low-Dose Gamma Irradiation on Inactivation of Escherichia coli O157:H7 and Salmonella enterica on Grape Tomatoes SO JOURNAL OF FOOD SCIENCE LA English DT Article DE E. coli O157:H7; gamma irradiation; integrated treatment; Salmonella; tomato; UV light ID LISTERIA-MONOCYTOGENES; FRESH PRODUCE; CHLORINATED WATER; DICED TOMATOES; SHELF-LIFE; VEGETABLES; LETTUCE; FRUITS; RADIATION; PATHOGENS AB The study evaluated the efficacy of integrated ultraviolet-C light (UVC) and low-dose gamma irradiation treatments to inactivate mixed strains of Escherichia coli O157:H7 and Salmonella enterica on inoculated whole grape tomatoes. A mixed bacterial cocktail composed of a 3 strain mixture of E. coli O157:H7 (C9490, E02128, and F00475) and a 3 serotype mixture of S. enterica (S. Montevideo G4639, S. Newport H1275, and S. Stanley H0558) was used based on their association with produce-related outbreaks. Spot inoculation (50 to 100 mL) on tomato surfaces was performed to achieve a population of appropriately 10(7-8) CFU/tomato. Inoculated tomatoes were subjected to UVC (253.7 nm) dose of 0.6 kJ/m(2) followed by 4 different low doses of gamma irradiations (0.1 kGy, 0.25 kGy, 0.5 kGy, 0.75 kGy). The fate of background microflora (mesophilic aerobic) including mold and yeast counts were also determined during storage at 5 degrees C over 21 d. Integrated treatment significantly (P < 0.05) reduced the population of target pathogens. Results indicate about 3.4 +/- 0.3 and 3.0 +/- 0.1 log CFU reduction of E. coli O157:H7 and S. enterica, respectively, per tomato with UVC (0.6 kJ/m(2)) and 0.25 kGy irradiation. More than a 4 log and higher reduction (>5 log) per fruit was accomplished by combined UVC treatment with 0.5 kGy and 0.75 kGy irradiation, respectively, for all tested pathogens. Furthermore, the combined treatment significantly (P < 0.05) reduced the native microflora compared to the control during storage. The data suggest efficacious treatment strategy for produce indicating 5 or higher log reduction which is consistent with the recommendations of the Natl. Advisory Committee on Microbiological Criteria for Foods. C1 [Mukhopadhyay, S.; Fan, X.; Juneja, V. K.] ARS, Residue Chem & Predicd Microbiol Res Unit, USDA, Res Ctr, Wyndmoor, PA 19038 USA. [Ukuku, D.] ARS, Food Safety & Intervent Technol Res Unit, USDA, Res Ctr, Wyndmoor, PA 19038 USA. RP Mukhopadhyay, S (reprint author), ARS, Residue Chem & Predicd Microbiol Res Unit, USDA, Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Sudarsan.mukhopadhyay@ars.usda.gov NR 60 TC 1 Z9 1 U1 2 U2 39 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 J9 J FOOD SCI JI J. Food Sci. PD JUL PY 2013 VL 78 IS 7 BP M1049 EP M1056 DI 10.1111/1750-3841.12154 PG 8 WC Food Science & Technology SC Food Science & Technology GA 185HN UT WOS:000321958400015 PM 23701667 ER PT J AU Sebastian, RS Enns, CW Steinfeldt, LC Goldman, JD Moshfegh, AJ AF Sebastian, Rhonda S. Enns, Cecilia Wilkinson Steinfeldt, Lois C. Goldman, Joseph D. Moshfegh, Afanna J. TI Monitoring Sodium Intake of the US Population: Impact and Implications of a Change in What We Eat in America, National Health and Nutrition Examination Survey Dietary Data Processing SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Sodium intake; Data comparability; What We Eat in America/National Health and Nutrition Examination Survey (WWEIA/NHANES) AB Accurate monitoring of US sodium intake requires familiarity with national dietary data collection and processing procedures. This article describes a data processing step that impacts sodium intake estimates, reasons for discontinuing the step, and implications of its discontinuation. This step, termed salt adjustment, was performed in US Department of Agriculture (USDA) dietary intake surveys from 1985 through 2008. In What We Eat in America (WWEIA), the dietary intake interview component of the National Health and Nutrition Examination Survey (NHANES), the salt content of specific foods was reduced on the basis of a question about household use of salt in cooking. For individuals whose households used salt in cooking occasionally or less often, some or all of the salt attributable to home preparation was removed from foods that typically have salt added during preparation and were obtained from the store. The growing availability of preprepared foods in stores challenges the validity of using store purchase as a proxy indicator of home food preparation, and increased restaurant/fast-food consumption implies fewer reported foods are eligible for the procedure. In addition, USDA's Automated Multiple-Pass Method for the 24-hour dietary recall provides accurate sodium intake estimates without applying the salt-adjustment step. The final WWEIA, NHANES data release to contain salt-adjusted sodium data was 2007-2008. When assessing the effectiveness of sodium-reduction efforts over time, the nutrition community (eg, researchers, analysts, providers) must be aware of this change in WWEIA, NHANES beginning in 2009-2010 and account for it using appropriate baseline estimates. C1 [Sebastian, Rhonda S.; Enns, Cecilia Wilkinson; Steinfeldt, Lois C.; Goldman, Joseph D.; Moshfegh, Afanna J.] ARS, USDA, Food Surveys Res Grp, Beltsville, MD 20705 USA. RP Sebastian, RS (reprint author), ARS, USDA, Food Surveys Res Grp, BARC West, 10300 Baltimore Ave,Bldg 005,Room 102, Beltsville, MD 20705 USA. EM Rhonda.Sebastian@ars.usda.gov NR 30 TC 9 Z9 10 U1 0 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD JUL PY 2013 VL 113 IS 7 BP 942 EP 949 DI 10.1016/j.jand.2013.02.009 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 183FI UT WOS:000321799300010 PM 23567248 ER PT J AU Swale, DR Tong, F Temeyer, KB Li, A Lam, PCH Totrov, MM Carlier, PR de Leon, AAP Bloomquist, JR AF Swale, Daniel R. Tong, Fan Temeyer, Kevin B. Li, Andrew Lam, Polo C-H. Totrov, Maxim M. Carlier, Paul R. de Leon, Adalberto A. Perez Bloomquist, Jeffrey R. TI Inhibitor profile of bis(n)-tacrines and N-methylcarbamates on acetylcholinesterase from Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi SO PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Leishmaniasis; Sandfly; Cattle fever; Southern cattle tick; Insecticides; Selectivity ID PERIPHERAL SITE; CATTLE TICK; SANDFLY POPULATIONS; RESISTANCE; BINDING; ORGANOPHOSPHORUS; LEISHMANIASIS; ACARICIDES; TOXICITY; IXODIDAE AB The cattle tick, Rhipicephalus (Boophilus) microplus (Bm), and the sand fly, Phlebotomus papatasi (Pp), are disease vectors to cattle and humans, respectively. The purpose of this study was to characterize the inhibitor profile of acetylcholinesterases from Bm (BmAChE1) and Pp (PpAChE) compared to human and bovine AChE, in order to identify divergent pharmacology that might lead to selective inhibitors. Results indicate that BmAChE has low sensitivity (IC50 = 200 mu M) toward tacrine, a monovalent catalytic site inhibitor with sub micromolar blocking potency in all previous species tested. Similarly, a series of bis(n)-tacrine dimer series, bivalent inhibitors and peripheral site AChE inhibitors possess poor potency toward BmAChE. Molecular homology models suggest the rBmAChE enzyme possesses a W384F orthologous substitution near the catalytic site, where the larger tryptophan side chain obstructs the access of larger ligands to the active site, but functional analysis of this mutation suggests it only partially explains the low sensitivity to tacrine. In addition, BmAChE1 and PpAChE have low nanomolar sensitivity to some experimental carbamate anticholinesterases originally designed for control of the malaria mosquito, Anopheles gambiae. One experimental compound, 2-((2-ethylbutyl)thio)phenyl methylcarbamate, possesses >300-fold selectivity for BmAChE1 and PpAChE over human AChE, and a mouse oral LD50 of >1500 mg/kg, thus providing an excellent new lead for vector control. (C) 2013 Elsevier Inc. All rights reserved. C1 [Swale, Daniel R.; Tong, Fan; Bloomquist, Jeffrey R.] Univ Florida, Dept Entomol & Nematol, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Temeyer, Kevin B.; Li, Andrew; de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Lam, Polo C-H.; Totrov, Maxim M.] Molsoft LLC, La Jolla, CA 92037 USA. [Carlier, Paul R.] Virginia Tech, Dept Chem, Blacksburg, VA 24061 USA. RP Bloomquist, JR (reprint author), Univ Florida, Dept Entomol & Nematol, Emerging Pathogens Inst, Gainesville, FL 32611 USA. EM jbquist@epi.ufl.edu FU National Institutes of Health [AI082581]; Foundation for the National Institutes of Health Grand Challenges in Global Health Initiative [GCGH-1497]; United States Department of Agriculture [108-0128-098]; Deployed War-fighter Protection Research Program; Virginia Tech (College of Science); Virginia Tech (Department of Chemistry); Virginia Tech (Fralin Life Science Institute) FX We thank the National Institutes of Health (AI082581), the Foundation for the National Institutes of Health (GCGH-1497) through the Grand Challenges in Global Health Initiative, the United States Department of Agriculture (Specific Cooperative Agreement 108-0128-098) and Deployed War-fighter Protection Research Program, as well as Virginia Tech (the College of Science, the Department of Chemistry, and Fralin Life Science Institute) for financial support of this work. We would also like to thank Kristie Schlechte for excellent technical assistance with the tick recombinant enzymes, and Dr. James Mutunga for his part of the mouse oral toxicity studies. The USDA is an equal opportunity provider and employer. NR 37 TC 7 Z9 7 U1 1 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0048-3575 J9 PESTIC BIOCHEM PHYS JI Pest. Biochem. Physiol. PD JUL PY 2013 VL 106 IS 3 SI SI BP 85 EP 92 DI 10.1016/j.pestbp.2013.03.005 PG 8 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 183HM UT WOS:000321805100004 ER PT J AU Temeyer, KB Olafson, PU Brake, DK Tuckow, AP Li, AY de Leon, AAP AF Temeyer, Kevin B. Olafson, Pia U. Brake, Danett K. Tuckow, Alexander P. Li, Andrew Y. de Leon, Adalberto A. Perez TI Acetylcholinesterase of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression, and biochemical properties of recombinant proteins SO PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE Acetylcholinesterase; Organophosphate resistance; Cattle fever; Piroplasmosis; Southern cattle tick; Babesiosis; Anaplasmosis; Sand fly; Leishmaniasis ID ORGANOPHOSPHATE-RESISTANT; INSECT ACETYLCHOLINESTERASE; SUSCEPTIBLE STRAINS; CIMEX-LECTULARIUS; SAND FLY; BED BUG; IXODIDAE; ACARI; CDNA; COUMAPHOS AB The southern cattle tick Rhipicephalus (Boophilus) micro plus (Bm) is a vector of bovine babesiosis and anaplasmosis. Tick resistance to organophosphate (OP) acaricide involves acetylcholinesterase (AChE) insensitivity to OP and metabolic detoxification. Sequencing and in vitro expression of Bin genes encoding AChE allowed biochemical characterization of three BmAChEs expressed in tick synganglion. rBmAChE1, rBmAChE2 and rBmAChE3 exhibited substrate preference for acetylthiocholine, high substrate inhibition and sensitivity to AChE-specific inhibitors. OP-insensitivity mutations were demonstrated in rBmAChE1 and rBmAChE3. Gene silencing suggested functional complementation of BmAChEs in vivo. BmAChE genes were amplified in copy number and multiple transcript polymorphisms were expressed in individual tick synganglia for each of the BmAChEs, suggesting allelic diversity within individuals. Studies also identified a gene encoding AChE of the sand fly, Phlebotomus papatasi, a vector of leishmaniasis in humans and animals. Expression of recombinant P. papatasi AChE (rPpAChE) enabled biochemical characterization and identification of effective inhibitors that selectively target rPpAChE. C1 [Temeyer, Kevin B.; Olafson, Pia U.; Brake, Danett K.; Tuckow, Alexander P.; Li, Andrew Y.; de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Temeyer, KB (reprint author), USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM kevin.temeyer@ars.usda.gov; pia.olafson@ars.usda.gov; danett.bishop@med.navy.mil; alex.tuckow@ars.usda.gov; andrew.li@ars.usda.gov; beto.perezdeleon@ars.usda.gov FU USDA appropriated funds (CRIS) [6205-32000-033000D]; USDA-ARS CRADA [60-0208-9-601, 6201-32000-033017R]; Deployed Warfighter Protection Research Program of the U.S. Department of Defense through the Armed Forces Pest Management Board [6205-32000-033-20]; USDA, ARS, Postdoctoral Research Associate Program FX The authors thank Kristie Schlechte, Darci Burchers, and DeEsta Hyatt for excellent technical assistance. This work was funded by USDA appropriated funds (CRIS # 6205-32000-033000D) and USDA-ARS CRADA number 60-0208-9-601 (Project number 6201-32000-033017R), and fund (6205-32000-033-20) from Deployed Warfighter Protection Research Program of the U.S. Department of Defense through the Armed Forces Pest Management Board: A.P. Tuckow is supported by a USDA, ARS, Postdoctoral Research Associate Program award to K.B. Temeyer. NR 50 TC 6 Z9 6 U1 0 U2 17 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0048-3575 J9 PESTIC BIOCHEM PHYS JI Pest. Biochem. Physiol. PD JUL PY 2013 VL 106 IS 3 SI SI BP 118 EP 123 DI 10.1016/j.pestbp.2013.01.005 PG 6 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 183HM UT WOS:000321805100008 ER PT J AU Dickens, JC Bohbot, JD AF Dickens, Joseph C. Bohbot, Jonathan D. TI Mini review: Mode of action of mosquito repellents SO PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY LA English DT Review DE Insect repellent; Feeding deterrent; Olfaction; Gustation; Modulation ID INSECT ODORANT RECEPTOR; AEDES-AEGYPTI; OLFACTORY RECEPTORS; ANOPHELES-GAMBIAE; MAXILLARY PALP; CARBON-DIOXIDE; ION CHANNELS; DEET; CHEMORECEPTORS; OVIPOSITION AB The mode of action of mosquito repellents remains a controversial topic. However, electrophysiological studies and molecular approaches have provided a better understanding of how repellents exert their effects. Here, we briefly discuss various theories of repellent action and present the current status of knowledge of the effects of repellents on olfactory and gustatory processes. These findings provide a framework for further development of existing repellents and the discovery of new compounds with novel modes of action. Published by Elsevier Inc. C1 [Dickens, Joseph C.; Bohbot, Jonathan D.] ARS, USDA, Henry A Wallace Beltsville Agr Res Ctr, Plant Sci Inst,Invas Insect Biocontrol & Behav La, Beltsville, MD 20705 USA. RP Dickens, JC (reprint author), ARS, USDA, BARC, PSI,IIBBL, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM jdickdickens@comcast.net FU Deployed War Fighter Protection (DWFP) Research Program FX The authors are grateful for reviews of the manuscript by Dr. Daniel Strickman, USDA, ARS, Beltsville, MD and Dr. Jackson Sparks, University of South Carolina. This work was supported in part by a grant to JCD from the Deployed War Fighter Protection (DWFP) Research Program. NR 40 TC 24 Z9 24 U1 5 U2 53 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0048-3575 J9 PESTIC BIOCHEM PHYS JI Pest. Biochem. Physiol. PD JUL PY 2013 VL 106 IS 3 SI SI BP 149 EP 155 DI 10.1016/j.pestbp.2013.02.006 PG 7 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA 183HM UT WOS:000321805100011 ER PT J AU Qin, H Gu, Q Kuppu, S Sun, L Zhu, XL Mishra, N Hu, RB Shen, GX Zhang, JL Zhang, YZ Zhu, LF Zhang, XL Burow, M Payton, P Zhang, H AF Qin, Hua Gu, Qiang Kuppu, Sundaram Sun, Li Zhu, Xunlu Mishra, Neelam Hu, Rongbin Shen, Guoxin Zhang, Junling Zhang, Yizheng Zhu, Longfu Zhang, Xianlong Burow, Mark Payton, Paxton Zhang, Hong TI Expression of the Arabidopsis vacuolar H+-pyrophosphatase gene AVP1 in peanut to improve drought and salt tolerance SO PLANT BIOTECHNOLOGY REPORTS LA English DT Article DE Drought tolerance; Peanut transformation; Salt resistance; Transgenic plants; Yield improvement ID GENETICALLY-ENGINEERED PLANTS; ARACHIS-HYPOGAEA L.; SCIENTISTS ANALYSIS; FIELD CONDITIONS; WATER; YIELD; PPASE; HEAT; OVEREXPRESSION; EFFICIENCY AB The Arabidopsis gene AVP1 encodes an H+-pyrophosphatase that functions as a proton pump at the vacuolar membranes, generating a proton gradient across vacuolar membranes, which serves as the driving force for many secondary transporters on vacuolar membranes such as Na+/H+-antiporters. Overexpression of AVP1 could improve drought tolerance and salt tolerance in transgenic plants, suggesting a possible way in improving drought and salt tolerance in crops. The AVP1 was therefore introduced into peanut by Agrobacterium-mediated transformation. Analysis of AVP1-expressing peanut indicated that AVP1-overexpression in peanut could improve both drought and salt tolerance in greenhouse and growth chamber conditions, as AVP1-overexpressing peanuts produced more biomass and maintained higher photosynthetic rates under both drought and salt conditions. In the field, AVP1-overexpressing peanuts also outperformed wild-type plants by having higher photosynthetic rates and producing higher yields under low irrigation conditions. C1 [Qin, Hua; Zhang, Yizheng] Sichuan Univ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China. [Gu, Qiang; Kuppu, Sundaram; Sun, Li; Zhu, Xunlu; Mishra, Neelam; Hu, Rongbin; Zhang, Hong] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. [Shen, Guoxin] Zhejiang Acad Agr Sci, Hangzhou, Zhejiang, Peoples R China. [Zhang, Junling; Burow, Mark] Texas A&M Univ, Texas AgriLife Res Ctr, Lubbock, TX 79403 USA. [Zhu, Longfu; Zhang, Xianlong] Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Hubei, Peoples R China. [Payton, Paxton] ARS, USDA, Cropping Syst Res Lab, Lubbock, TX 79415 USA. RP Zhang, H (reprint author), Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. EM hong.zhang@ttu.edu RI HU, RONGBIN/B-2225-2017; OI HU, RONGBIN/0000-0001-5921-6891 FU National Peanut Board; Texas Peanut Producers Board; China Scholarship Council FX This research was supported by Grants from National Peanut Board and Texas Peanut Producers Board. Hua Qin thanks the China Scholarship Council for a 1-year fellowship to study abroad. NR 34 TC 14 Z9 14 U1 1 U2 47 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1863-5466 J9 PLANT BIOTECHNOL REP JI Plant Biotechnol. Rep. PD JUL PY 2013 VL 7 IS 3 BP 345 EP 355 DI 10.1007/s11816-012-0269-5 PG 11 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA 184NF UT WOS:000321896900015 ER PT J AU Plaza, N Zelinka, SL Stone, DS Jakes, JE AF Plaza, Nayomi Zelinka, Samuel L. Stone, Don S. Jakes, Joseph E. TI Plant-based torsional actuator with memory SO SMART MATERIALS AND STRUCTURES LA English DT Article ID MOISTURE-CONTENT; WOOD; SHAPE; COMPONENTS; MODULUS; MUSCLES; TENSILE; SYSTEMS; LIGNIN; POINT AB A bundle of a few loblolly pine (Pinus taeda) cells are moisture-activated torsional actuators that twist multiple revolutions per cm length in direct proportion to moisture content. The bundles generate 10 N m kg(-1) specific torque during both twisting and untwisting, which is higher than an electric motor. Additionally, the bundles exhibit a moisture-activated, shape memory twist effect. Over 70% of the twist in a wetted bundle can be locked-in by drying under constraint and then released by rewetting the bundle. Our results indicate that hemicelluloses dominate the shape fixity mechanism and lignin is primarily responsible for remembering the bundle's original form. The bundles demonstrate proof of a high specific torque actuator with large angles of rotation and shape memory twist capabilities that can be used in microactuators, sensors, and energy harvesters. C1 [Plaza, Nayomi; Zelinka, Samuel L.; Jakes, Joseph E.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. [Plaza, Nayomi; Stone, Don S.] Univ Wisconsin, Mat Sci Program, Madison, WI 53706 USA. [Stone, Don S.] Univ Wisconsin, Dept Mat Sci & Engn, Madison, WI 53706 USA. RP Plaza, N (reprint author), US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. EM jjakes@fs.fed.us RI Stone, Donald/A-7496-2016; OI Plaza Rodriguez, Nayomi/0000-0002-5198-4877 FU PECASE FX NP acknowledges the SURE-REU program at UW-Madison for providing the initial opportunity to conduct this research during summer 2011 and the GERS program at UW-Madison and 2012 NSF GFRP for graduate support to continue the research. SLZ and JEJ acknowledge PECASE funding. The authors also thank Karen Nelson in the Office of Communications of FPL for the illustration in figure 1(C), Tom Kuster in the Analytical Chemistry and Microscopy Laboratory at FPL for the SEM images in figures 1(A) and (B), and Professors Rod Lakes and Izabela Szlufarska at UW-Madison for manuscript review and helpful discussions. NR 37 TC 7 Z9 7 U1 3 U2 24 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 0964-1726 J9 SMART MATER STRUCT JI Smart Mater. Struct. PD JUL PY 2013 VL 22 IS 7 AR 072001 DI 10.1088/0964-1726/22/7/072001 PG 7 WC Instruments & Instrumentation; Materials Science, Multidisciplinary SC Instruments & Instrumentation; Materials Science GA 172VU UT WOS:000321034000001 ER PT J AU Rosa, LH Queiroz, SCN Moraes, RM Wang, XN Techen, N Pan, ZQ Cantrell, CL Wedge, DE AF Rosa, Luiz H. Queiroz, Sonia C. N. Moraes, Rita M. Wang, Xiaoning Techen, Natascha Pan, Zhiqiang Cantrell, Charles L. Wedge, David E. TI Coniochaeta ligniaria: antifungal activity of the cryptic endophytic fungus associated with autotrophic tissue cultures of the medicinal plant Smallanthus sonchifolius (Asteraceae) SO SYMBIOSIS LA English DT Article DE Antifungal; Fatty acids; Medicinal plant; Plant tissue culture; Symbiosis ID LECYTHOPHORA-HOFFMANNII; FATTY-ACIDS; BIOLOGICAL-CONTROL; LEAF EXTRACTS; DERIVATIVES; DIVERSITY; SYMBIOSIS; AGENTS; WOOD AB Few studies have addressed the presence and bioactivity of endophytic fungi living in plantlets growing under in vitro conditions. After unfruitful attempts to grow axenic shoot cultures of the medicinal plant Smallanthus sonchifolius (yacon) were made, healthy shoots grew on half strength Murashigue and Skoog media supplemented with 2.2 mu M benzylaminopurine without sucrose. We isolated a fungus UM109 from these autotrophic tissue cultures and it was identified as Coniochaeta ligniaria using molecular, physiological and morphological methods. Dichloromethane extracts from C. ligniaria and its host S. sonchifolius exhibited antifungal activity against phytopathogenic fungi Colletotrichum acutatum, C. fragariae and C. gloeosporioides. Both extracts of C. ligniaria and S. sonchifolius were subjected to antifungal bioassay-directed fractionation using NMR spectroscopy and GC-FID analysis. Twelve antifungal fatty acids were identified and 8 out of the total were produced by the fungus and the plant including caproic, caprylic, myristic, palmitic, heptadecanoic, stearic, oleic and linoleic acids. Additionally, caproic, caprylic and palmitic acids were produced at high concentrations by the endophytic fungus and its host. The detection of these antifungal fatty acids produced by both C. ligniaria and S. sonchifolius suggests that these bioactive compounds may be partially responsible for the high resistance of S. sonchifolius to phytopathogenic fungal attacks. This finding also indicates the existence of an interesting chemical symbiosis between an endophytic fungus and its host. Furthermore, the isolation of C. ligniaria from tissue culture of S. sonchifolius demonstrates that plantlets growing in vitro as autotrophic cultures can shelter specific endophytic fungal communities. The use of autotrophic tissue cultures may become an important tool for studies on the taxonomy, ecology, evolution and biotechnological application of endophytes. C1 [Rosa, Luiz H.] Univ Fed Minas Gerais, Lab Sistemat & Biomol Fungos, Dept Microbiol, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil. [Queiroz, Sonia C. N.] EMBRAPA Meio Ambiente, BR-13820000 Jaguariuna, SP, Brazil. [Moraes, Rita M.; Techen, Natascha] Univ Mississippi, Natl Ctr Nat Prod Res, Pharmaceut Sci Res Inst, Sch Pharm, University, MS 38677 USA. [Moraes, Rita M.] Univ Mississippi, Ctr Water & Wetland Resources, Biol Field Stn, Abbeville, MS 38601 USA. [Wang, Xiaoning] Univ Mississippi, Dept Med Chem, University, MS 38677 USA. [Pan, Zhiqiang; Cantrell, Charles L.; Wedge, David E.] USDA ARS, NPURU, University, MS 38677 USA. RP Rosa, LH (reprint author), Univ Fed Minas Gerais, Lab Sistemat & Biomol Fungos, Dept Microbiol, Inst Ciencias Biol, POB 486, BR-31270901 Belo Horizonte, MG, Brazil. EM lhrosa@icb.ufmg.br; dwedge@olemiss.edu RI Queiroz, Sonia/G-9714-2012 FU Conselho Nacional of the Desenvolvimento Cientifico and Tecnologico [200774/2011-5]; Fundacao of Amparo the Pesquisa of Minas Gerais (FAPEMIG); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior [CAPES/PGCI 036/13]; USDA, Agricultural Research Service [58-6408-2-0009] FX This work received support of the Conselho Nacional of the Desenvolvimento Cientifico and Tecnologico (processo 200774/2011-5), Fundacao of Amparo the Pesquisa of Minas Gerais (FAPEMIG), and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES/PGCI 036/13). The authors thank Ms. J.L. Robertson, Mrs. R. Pace, Mrs. Amber Reichley, Mr. Solomon Green III and Mrs. Marilyn Ruscoe for technical support. We also thank Dr. N. Tabanca and Dr. A. L. Cerdeira for valuable comments on the manuscript. The National Center for Natural Products Research, University of Mississippi is partially supported through a cooperative scientific agreement 58-6408-2-0009 from the USDA, Agricultural Research Service. NR 44 TC 8 Z9 8 U1 2 U2 35 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0334-5114 EI 1878-7665 J9 SYMBIOSIS JI Symbiosis PD JUL PY 2013 VL 60 IS 3 BP 133 EP 142 DI 10.1007/s13199-013-0249-8 PG 10 WC Microbiology SC Microbiology GA 186BW UT WOS:000322017600004 ER PT J AU Maragos, CM Kurtzman, C Busman, M Price, N McCormick, S AF Maragos, Chris M. Kurtzman, Cletus Busman, Mark Price, Neil McCormick, Susan TI Development and Evaluation of Monoclonal Antibodies for the Glucoside of T-2 Toxin (T2-Glc) SO TOXINS LA English DT Article DE T-2 toxin; masked mycotoxins; metabolites; antibody; immunoassay ID LINKED-IMMUNOSORBENT-ASSAY; PERFORMANCE LIQUID-CHROMATOGRAPHY; FUSARIUM MASKED MYCOTOXINS; TANDEM MASS-SPECTROMETRY; CROSS-REACTIVITY; HT-2 TOXIN; A TRICHOTHECENES; DEOXYNIVALENOL; WHEAT; IMMUNOASSAY AB The interactions between fungi and plants can yield metabolites that are toxic in animal systems. Certain fungi are known to produce sesquiterpenoid trichothecenes, such as T-2 toxin, that are biotransformed by several mechanisms including glucosylation. The glucosylated forms have been found in grain and are of interest as potential reservoirs of T-2 toxin that are not detected by many analytical methods. Hence the glucosides of trichothecenes are often termed "masked" mycotoxins. The glucoside of T-2 toxin (T2-Glc) was linked to keyhole limpet hemocyanin and used to produce antibodies in mice. Ten monoclonal antibody (Mab)-producing hybridoma cell lines were developed. The Mabs were used in immunoassays to detect T2-Glc and T-2 toxin, with midpoints of inhibition curves (IC(50)s) in the low ng/mL range. Most of the Mabs demonstrated good cross-reactivity to T-2 toxin, with lower recognition of HT-2 toxin. One of the clones (2-13) was further characterized with in-depth cross-reactivity and solvent tolerance studies. Results suggest Mab 2-13 will be useful for the simultaneous detection of T-2 toxin and T2-Glc. C1 [Maragos, Chris M.; Kurtzman, Cletus; Busman, Mark; McCormick, Susan] USDA ARS NCAUR, Bacterial Foodborne Pathogens & Mycol Res Unit, Peoria, IL 61604 USA. [Price, Neil] USDA ARS NCAUR, Renewable Product Technol Res Unit, Peoria, IL 61604 USA. RP Maragos, CM (reprint author), USDA ARS NCAUR, Bacterial Foodborne Pathogens & Mycol Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM chris.maragos@ars.usda.gov; cletus.kurtzman@ars.usda.gov; mark.busman@ars.usda.gov; neil.price@ars.usda.gov; susan.mccormick@ars.usda.gov NR 49 TC 7 Z9 7 U1 3 U2 29 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD JUL PY 2013 VL 5 IS 7 BP 1299 EP 1313 DI 10.3390/toxins5071299 PG 15 WC Toxicology SC Toxicology GA 188HW UT WOS:000322184700006 PM 23877196 ER PT J AU Roth, WJ Kissinger, CB McCain, RR Cooper, BR Marchant-Forde, JN Vreeman, RC Hannou, S Knipp, GT AF Roth, Wyatt J. Kissinger, Candice B. McCain, Robyn R. Cooper, Bruce R. Marchant-Forde, Jeremy N. Vreeman, Rachel C. Hannou, Sophia Knipp, Gregory T. TI Assessment of Juvenile Pigs to Serve as Human Pediatric Surrogates for Preclinical Formulation Pharmacokinetic Testing SO AAPS JOURNAL LA English DT Article DE ADME; pediatric; pharmacokinetics; porcine; rifampin ID RIFAMPICIN SERUM-LEVELS; DRUG DEVELOPMENT; IN-VITRO; CHILDREN; MEDICINES; TUBERCULOSIS; RIFAMYCIN; CYTOCHROME-P450; BIOAVAILABILITY; METABOLISM AB Pediatric drug development is hampered by biological, clinical, and formulation challenges associated with age-based populations. A primary cause for this lack of development is the inability to accurately predict ontogenic changes that affect pharmacokinetics (PK) in children using traditional preclinical animal models. In response to this issue, our laboratory has conducted a proof-of-concept study to investigate the potential utility of juvenile pigs to serve as surrogates for children during preclinical PK testing of selected rifampin dosage forms. Pigs were surgically modified with jugular vein catheters that were externalized in the dorsal scapular region and connected to an automated blood sampling system (PigTurn-Culex-L). Commercially available rifampin capsules were administered to both 20 and 40 kg pigs to determine relevant PK parameters. Orally disintegrating tablet formulations of rifampin were also developed and administered to 20 kg pigs. Plasma samples were prepared from whole blood by centrifugation and analyzed for rifampin content by liquid chromatography-tandem mass spectrometry. Porcine PK parameters were determined from the resultant plasma-concentration time profiles and contrasted with published rifampin PK data in human adults and children. Results indicated significant similarities in dose-normalized absorption and elimination parameters between pigs and humans. Moreover, ontogenic changes observed in porcine PK parameters were consistent with ontogenic changes reported for human PK. These results demonstrate the potential utility of the juvenile porcine model for predicting human pediatric PK for rifampin. Furthermore, utilization of juvenile pigs during formulation testing may provide an alternative approach to expedite reformulation efforts during pediatric drug development. C1 [Roth, Wyatt J.; Kissinger, Candice B.; Hannou, Sophia; Knipp, Gregory T.] Purdue Univ, Coll Pharm, Dept Ind & Phys Pharm, W Lafayette, IN 47907 USA. [McCain, Robyn R.; Cooper, Bruce R.; Knipp, Gregory T.] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA. [McCain, Robyn R.; Marchant-Forde, Jeremy N.; Knipp, Gregory T.] Purdue Univ, Purdue Translat Pharmacol CTSI Core Facil, W Lafayette, IN 47907 USA. [Marchant-Forde, Jeremy N.] ARS, Livestock Behav Res Unit, USDA, W Lafayette, IN USA. [Vreeman, Rachel C.] Indiana Univ, Sch Med, Dept Pediat, Indianapolis, IN 46202 USA. RP Knipp, GT (reprint author), Purdue Univ, Coll Pharm, Dept Ind & Phys Pharm, 575 Stadium Mall Dr, W Lafayette, IN 47907 USA. EM gknipp@purdue.edu OI Marchant-Forde, Jeremy/0000-0002-5287-2914 FU Indiana Clinical and Translational Sciences Institute (NIH) [RR025761]; Dane O. Kildsig Center for Pharmaceutical Processing and Research; Lilly Endowment Graduate Research Fellowship; Purdue Translational Pharmacology (PTP) Core Facility of the Clinical Translational Sciences Award (Core Pilot Funding NIH) [RR025761] FX The authors would like to acknowledge the financial support received from the Indiana Clinical and Translational Sciences Institute (NIH Grant # RR025761, Dr. Knipp and Dr. Vreeman), the Dane O. Kildsig Center for Pharmaceutical Processing and Research, and the 2011-2012 Lilly Endowment Graduate Research Fellowship for Mr. Wyatt Roth. The authors would like to acknowledge that the porcine studies were conducted in the Purdue Translational Pharmacology (PTP) Core Facility of the Clinical Translational Sciences Award (Core Pilot Funding NIH Grant # RR025761). In addition, we would also like to thank Bioanalytical Systems, Inc. and Dr. Jeremy Marchant-Forde for providing access to the PigTurn-Culex-L (R) units. We would also like to acknowledge the assistance of Dr. Lee Matthews from the Purdue Office of the Vice President for Research for serving as the staff veterinarian for these studies. Finally, we thank Drs. Peter Kissinger, Carmen Popescu, and Rodolfo Pinal for their helpful suggestions and assistance on the project. NR 71 TC 10 Z9 10 U1 2 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1550-7416 J9 AAPS J JI AAPS J. PD JUL PY 2013 VL 15 IS 3 BP 763 EP 774 DI 10.1208/s12248-013-9482-6 PG 12 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA 172XU UT WOS:000321041000014 PM 23595360 ER PT J AU Mathews, KH Jones, KG McConnell, MJ Johnson, RJ AF Mathews, Kenneth H., Jr. Jones, Keithly G. McConnell, Michael J. Johnson, Rachel J. TI Trade-adjusted measures of productivity increases in US hog production (vol 114, pg 32, 2013) SO AGRICULTURAL SYSTEMS LA English DT Correction C1 [Mathews, Kenneth H., Jr.; Jones, Keithly G.; Johnson, Rachel J.] Econ Res Serv, USDA, Markets & Trade Econ Div, Washington, DC 20024 USA. [McConnell, Michael J.] US Int Trade Commiss, Washington, DC USA. RP Jones, KG (reprint author), Econ Res Serv, USDA, Patriots Plaza 3,355 E St SW, Washington, DC 20024 USA. EM kjones@ers.usda.gov NR 1 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-521X J9 AGR SYST JI Agric. Syst. PD JUL PY 2013 VL 119 BP 43 EP 43 DI 10.1016/j.agsy.2013.03.001 PG 1 WC Agriculture, Multidisciplinary SC Agriculture GA 179RA UT WOS:000321537800005 ER PT J AU Shea, MK Booth, SL Miller, ME Burke, GL Chen, HY Cushman, M Tracy, RP Kritchevsky, SB AF Shea, M. Kyla Booth, Sarah L. Miller, Michael E. Burke, Gregory L. Chen, Haiying Cushman, Mary Tracy, Russell P. Kritchevsky, Stephen B. TI Association between circulating vitamin K1 and coronary calcium progression in community-dwelling adults: the Multi-Ethnic Study of Atherosclerosis SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID MATRIX GLA PROTEIN; K BIOCHEMICAL MEASURES; ALL-CAUSE MORTALITY; ARTERY CALCIUM; PHYLLOQUINONE VITAMIN-K-1; COMPUTED-TOMOGRAPHY; DIETARY-INTAKE; RISK-FACTORS; CALCIFICATION; DISEASE AB Background: Animal studies have shown that vitamin K treatment reduced vascular calcification, but human data are limited. Objective: We determined the association between vitamin K status and coronary artery calcium (CAC) progression in the Multi-Ethnic Study of Atherosclerosis by using a case-cohort design. Design: Serum phylloquinone (vitamin K1) was measured in 296 participants with extreme CAC progression and 561 randomly selected participants without extreme CAC progression; all subjects had baseline and follow-up CAC measures (mean follow-up: 2.5 y). A serum vitamin K1 concentration was considered low at <1.0 nmol/L (the distribution median). Outcomes were replicated by using post hoc per-protocol analyses of a vitamin K1 supplementation trial. Results: The OR (95% CI) for extreme CAC progression for subjects with low serum vitamin K1 compared with subjects without extreme CAC progression was 1.34 (0.94, 1.90; NS) when adjusted for demographics and confounders. A significant interaction between low vitamin K1 and antihypertension medication use was detected (P = 0.016). Hypertension medication users with low serum vitamin K1 were more likely to have extreme CAC progression than were medication users without extreme CAC progression [OR (95% CI): 2.37 (1.38, 4.09)]. In replication, baseline antihypertensive medication users in the supplementation group had less CAC progression than did those in the control group [adjusted mean SEM of the 3-y CAC change was +5 +/- 20 Agatston units (AU) in the vitamin K1 group (n = 40) and +44 +/- 13 AU in the placebo group (n = 49); P < 0.01] Conclusions: Although the point estimate of our primary analysis suggests low serum vitamin K1 is associated with greater CAC progression, the difference was NS. Low serum vitamin K1 was significantly associated with CAC progression in antihypertension medication users, which, to our knowledge, is a novel finding conditionally replicated by using an independent sample. Intervention trials are needed to determine whether improving serum vitamin K1 reduces CAC progression, especially in hypertensive individuals. C1 [Shea, M. Kyla; Kritchevsky, Stephen B.] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC 27157 USA. [Miller, Michael E.; Burke, Gregory L.; Chen, Haiying] Wake Forest Sch Med, Div Publ Hlth Sci, Winston Salem, NC 27157 USA. [Booth, Sarah L.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Cushman, Mary] Univ Vermont, Coll Med, Dept Med, Burlington, VT 05405 USA. [Cushman, Mary; Tracy, Russell P.] Univ Vermont, Coll Med, Dept Pathol & Biochem, Burlington, VT USA. RP Shea, MK (reprint author), Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC 27157 USA. EM kshea@wakehealth.edu OI Kritchevsky, Stephen/0000-0003-3336-6781 FU National Heart, Lung, and Blood Institute [N01-HC-95159, N01-HC-95169, RR024156, R01HL69272]; National Institute of Aging [R01AG14759]; Wake Forest University Claude D Pepper Older Americans Independence Center [P30A021332]; American Heart Association [09CRP2070013]; USDA, Agricultural Research Service [58-1950-7-707] FX Supported by the National Heart, Lung, and Blood Institute (contracts N01-HC-95159 through N01-HC-95169, RR024156, and R01HL69272), the National Institute of Aging (R01AG14759), the Wake Forest University Claude D Pepper Older Americans Independence Center (P30A021332), the American Heart Association (09CRP2070013), and the USDA, Agricultural Research Service (cooperative agreement 58-1950-7-707). NR 50 TC 13 Z9 14 U1 0 U2 9 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JUL PY 2013 VL 98 IS 1 BP 197 EP 208 DI 10.3945/ajcn.112.056101 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 171EZ UT WOS:000320909200024 PM 23719555 ER PT J AU Arthur, TM Ahmed, R Chase-Topping, M Kalchayanand, N Schmidt, JW Bono, JL AF Arthur, Terrance M. Ahmed, Rafiq Chase-Topping, Margo Kalchayanand, Norasak Schmidt, John W. Bono, James L. TI Characterization of Escherichia coli O157:H7 Strains Isolated from Supershedding Cattle SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID FIELD GEL-ELECTROPHORESIS; BOVINE FECAL SAMPLES; VIRULENCE FACTORS; FEEDLOT CATTLE; MULTIPLEX PCR; GENETIC DIVERSITY; O157-H7 STRAINS; BEEF-CATTLE; COLONIZATION; IDENTIFICATION AB Previous reports have indicated that a small proportion of cattle shedding high levels of Escherichia coli O157:H7 is the main source for transmission of this organism between animals. Cattle achieving a fecal shedding status of 10(4) CFU of E. coli O157:H7/gram or greater are now referred to as supershedders. The aim of this study was to investigate the contribution of E. coli O157:H7 strain type to supershedding and to determine if supershedding was restricted to a specific set of E. coli O157:H7 strains. Fecal swabs (n = 5,086) were collected from cattle at feedlots or during harvest. Supershedders constituted 2.0% of the bovine population tested. Supershedder isolates were characterized by pulsed-field gel electrophoresis (PFGE), phage typing, lineage-specific polymorphism assay (LSPA), Stx-associated bacteriophage insertion (SBI) site determination, and variant analysis of Shiga toxin, tir, and antiterminator Q genes. Isolates representing 52 unique PFGE patterns, 19 phage types, and 12 SBI clusters were obtained from supershedding cattle, indicating that there is no clustering to E. coli O157:H7 genotypes responsible for supershedding. While being isolated directly from cattle, this strain set tended to have higher frequencies of traits associated with human clinical isolates than previously collected bovine isolates with respect to lineage and tir allele, but not for SBI cluster and Q type. We conclude that no exclusive genotype was identified that was common to all supershedder isolates. C1 [Arthur, Terrance M.; Kalchayanand, Norasak; Schmidt, John W.; Bono, James L.] USDA ARS, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Ahmed, Rafiq] Canadian Sci Ctr Human & Anim Hlth, Publ Hlth Agcy Canada, Winnipeg, MB, Canada. [Chase-Topping, Margo] Univ Edinburgh, Ctr Immun Infect & Evolut, Ashworth Labs, Edinburgh, Midlothian, Scotland. RP Arthur, TM (reprint author), USDA ARS, Roman L Hruska US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM Terrance.Arthur@ars.usda.gov OI Schmidt, John/0000-0003-0494-2436 FU Beef Checkoff FX This project was funded in part by The Beef Checkoff. NR 55 TC 15 Z9 15 U1 2 U2 27 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2013 VL 79 IS 14 BP 4294 EP 4303 DI 10.1128/AEM.00846-13 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 170LS UT WOS:000320852900012 PM 23645203 ER PT J AU Gerling, D Blackburn, MB AF Gerling, Dan Blackburn, Michael B. TI Immature development of Eretmocerus mundus (Hymenoptera: Aphelinidae) SO ARTHROPOD STRUCTURE & DEVELOPMENT LA English DT Article DE Whiteflies; Histology; Parasitoids; Digestive system ID INSECTS; ANATOMY; LARVAE AB The development from egg to pupation is followed for the wasp Eretmocerus mundus, parasitizing the whitefly Bemisia tabaci. We elucidate and describe structural details, histological developments and changes that the different parasitoid and host tissues have undergone during parasitism. These include the presence and apparent function of very large salivary glands, which probably produce substances that help to regulate the host's decomposition and parasitoid nutrition. Moreover, the gut of all instars is devoid of both peritrophic membrane and microvilli and, in the early instars, it has squamous rather than columnar epithelial cells. Differing from many other parasitoids, the E. mundus larva usually does not come into contact with the host tissues and does not devour the entire host during its development. The possible reasons for the developmental mechanisms, as well as the functions of the host capsule that envelopes the parasitoid, are discussed. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Gerling, Dan] Tel Aviv Univ, Dept Zool, IL-69978 Tel Aviv, Israel. [Blackburn, Michael B.] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. RP Gerling, D (reprint author), Tel Aviv Univ, Dept Zool, IL-69978 Tel Aviv, Israel. EM dange@tauex.tau.ac.il NR 31 TC 2 Z9 2 U1 2 U2 20 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1467-8039 EI 1873-5495 J9 ARTHROPOD STRUCT DEV JI Arthropod Struct. Dev. PD JUL PY 2013 VL 42 IS 4 BP 309 EP 314 DI 10.1016/j.asd.2013.03.003 PG 6 WC Entomology SC Entomology GA 174TF UT WOS:000321177600005 PM 23523739 ER PT J AU Center, TD Rayamajhi, M Dray, FA Madeira, PM Witkus, G Rohrig, E Mattison, E Lake, E Smith, M Zhang, JL Purcell, M Konstantinov, A Schmitz, D AF Center, Ted D. Rayamajhi, Min Dray, F. Allen Madeira, Paul M. Witkus, Gloria Rohrig, Eric Mattison, Elizabeth Lake, Ellen Smith, Melissa Zhang, Jialing Purcell, Matthew Konstantinov, Alex Schmitz, Don TI Host range validation, molecular identification and release and establishment of a Chinese biotype of the Asian leaf beetle Lilioceris cheni (Coleoptera: Chrysomelidae: Criocerinae) for control of Dioscorea bulbifera L. in the southern United States SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE weed biological control; invasive species; habitat restoration; insect-plant interactions; air potato; Dioscoreaceae ID BIOLOGICAL-CONTROL AGENT; COLORADO POTATO BEETLE; AIR-POTATO; INSECT HERBIVORE; DIAPAUSE; FLORIDA; MITOCHONDRIAL; EVOLUTIONARY; HYMENOPTERA; POPULATIONS AB Dioscorea bulbifera, an Asian vine, is invasive in the southeastern USA. It rarely flowers but propagates from potato-like bulbils formed in leaf axils, which persist into the subsequent growing season. Lilioceris cheni Gressitt and Kimoto, a foliage-feeding beetle (Coleoptera: Chrysomelidae: Criocerinae) from Nepal, had been tested, proven to be a specialist and approved for release as a biological control agent. Regulatory delays, however, resulted in the demise of quarantine-held colonies, and acquisition of new Nepalese stock proved untenable. Searches then undertaken in southern China resulted in the collection of over 300 similar beetles. Two Chinese Lilioceris species were identified: one confirmed to be L. cheni and the other identified as Lilioceris egena (Weise). Mitochondrial analysis revealed an exact DNA match between some Chinese and one of the two Nepalese c oxidase subunit I haplotypes and all Chinese L. cheni haplotypes clustered as a single species but the comingling of the two species aroused concerns over possible hybridisation. These concerns were allayed by nuclear D2 analysis showing the absence of dual parental sequences. Nonetheless, diligence was exercised to ensure that the Chinese strains were safe to release. Abridged host testing using critical test species verified specificity. Caged releases during autumn 2011 documented the ability of adult beetles to overwinter in south Florida despite a prolonged lack of foliage. Open releases the following year produced vigorous populations that caused extensive defoliation. Preliminary observations indicate that L. cheni now contributes to the control of D. bulbifera and the bulbil-feeding L. egena should complement these effects if its host range proves appropriate. C1 [Center, Ted D.; Rayamajhi, Min; Dray, F. Allen; Madeira, Paul M.; Witkus, Gloria; Mattison, Elizabeth; Lake, Ellen; Smith, Melissa] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL USA. [Rohrig, Eric] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL USA. [Zhang, Jialing] Chinese Acad Sci, Wuhan Bot Gardens, Lab Aquat Bot & Watershed Ecol, Wuhan, Hubei, Peoples R China. [Purcell, Matthew] USDA ARS, Australian Biol Control Lab, Brisbane, Qld, Australia. [Purcell, Matthew] CSIRO, Div Entomol, Brisbane, Qld, Australia. [Konstantinov, Alex] ARS, USDA, Systemat Entomol Lab, Washington, DC USA. [Schmitz, Don] Florida Fish & Wildlife Conservat Commiss, Tallahassee, FL USA. RP Rayamajhi, M (reprint author), ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL USA. EM Min.Rayamajhi@ars.usda.gov NR 57 TC 4 Z9 4 U1 1 U2 28 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD JUL 1 PY 2013 VL 23 IS 7 BP 735 EP 755 DI 10.1080/09583157.2013.790931 PG 21 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 184JR UT WOS:000321886400001 ER PT J AU Steininger, MS Wright, SA Ding, J Wheeler, GS AF Steininger, M. S. Wright, S. A. Ding, J. Wheeler, G. S. TI Biology and host range of Heterapoderopsis bicallosicollis: a potential biological control agent for Chinese tallow Triadica sebifera SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE Attelabidae; Euphorbiaceae; host range; specificity ID ATTELABIDAE; COLEOPTERA; AMERICA; NORTH AB Chinese tallow, Triadica sebifera, is an invasive weed that infests natural and agricultural areas of the south-eastern USA. A candidate insect for biological control of Chinese tallow has been studied under quarantine conditions. The biology and host range of a primitive leaf-feeding beetle, Heterapoderopsis bicallosicollis, were examined between July 2008 and February 2010. H. bicallosicollis adults fed on 16 of 29 non-target species and survived >10 days on all 29 species, more than the number of days that adults survived when deprived of food. Additionally, adult feeding, oviposition and partial larval development occurred on a Florida endangered species, Heterosavia bahamensis. These data indicated that H. bicallosicollis adult feeding and oviposition may not be limited to the target weed and included several valued North American natives. Therefore, as its release could pose unacceptable risks, testing of this species was discontinued and the quarantine colony destroyed. C1 [Steininger, M. S.; Wright, S. A.] ARS, USDA, Invas Plant Res Lab, Gainesville, FL USA. [Ding, J.] Chinese Acad Sci, Invas Biol & Biocontrol Lab, Wuhan Bot Garden, Wuhan, Peoples R China. [Wheeler, G. S.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL USA. RP Wheeler, GS (reprint author), ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL USA. EM greg.wheeler@ars.usda.gov FU Florida Fish and Wildlife Commission; Florida Department of Environmental Protection [SL849]; USDA/ARS FX We thank J. Gibson-Weston, K. Burchill and J. Lollis (USDA/ARS) for laboratory assistance. Weevils were identified by M. Thomas, FDACS DPI, A. Legalov, curator of Attelabidae, Siberian Zoological Museum, Russia and C. O'Brien, Greenvalley, Arizona. Caloptilia n. sp. (Gracillariidae) insect specimens were identified by D. Davis, Smithsonian Institute, Washington, DC. Adult beetle dissections were performed with the help of M. Thomas (FDACS DPI, Gainesville, FL, USA) and A. Legalov (Siberian Zoological Museum, Novosibirsk, Russia). Non-target plant species were graciously provided by D. Weaver, FDACS, DPI botanist, S. Hight, USDA/ARS/CMAVE, Tallahassee, FL, S. Woodmansee, Pro Native Consulting, Miami, FL, and G. Montgomery, Curator of Botany, Arizona Sonoran Desert Museum, Tucson, Arizona. Triadica sebifera seeds collected in AL, FL, GA, LA, MS, SC and TX were generously provided by E. Siemann, Rice University, Houston, Texas. Cuttings of T. sebifera were also provided by L. Battaglia, Southern Illinois University at Carbondale, Illinois. Voucher specimens of weevils are deposited at the Museum of Entomology, Division of Plant Industry, Gainesville, Florida. This research was funded by Florida Fish and Wildlife Commission and Florida Department of Environmental Protection (SL849 to GSW) and USDA/ARS. NR 18 TC 2 Z9 2 U1 1 U2 13 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD JUL 1 PY 2013 VL 23 IS 7 BP 816 EP 828 DI 10.1080/09583157.2013.797564 PG 13 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 184JR UT WOS:000321886400007 ER PT J AU Robertson, GP Bruulsema, TW Gehl, RJ Kanter, D Mauzerall, DL Rotz, CA Williams, CO AF Robertson, G. Philip Bruulsema, Tom W. Gehl, Ron J. Kanter, David Mauzerall, Denise L. Rotz, C. Alan Williams, Candiss O. TI Nitrogen-climate interactions in US agriculture SO BIOGEOCHEMISTRY LA English DT Article DE Nitrate; Nitrous oxide; Ozone; Greenhouse gases; Mitigation; Methane; Fertilizer ID GREENHOUSE-GAS MITIGATION; OXIDE EMISSIONS; SOIL CARBON; AMMONIA VOLATILIZATION; CROP PRODUCTION; NITRIC-OXIDE; LONG-TERM; FERTILIZER MANAGEMENT; AGRONOMIC ASSESSMENT; EXPERIMENTAL DROUGHT AB Agriculture in the United States (US) cycles large quantities of nitrogen (N) to produce food, fuel, and fiber and is a major source of excess reactive nitrogen (Nr) in the environment. Nitrogen lost from cropping systems and animal operations moves to waterways, groundwater, and the atmosphere. Changes in climate and climate variability may further affect the ability of agricultural systems to conserve N. The N that escapes affects climate directly through the emissions of nitrous oxide (N2O), and indirectly through the loss of nitrate (NO3 (-)), nitrogen oxides (NO (x) ) and ammonia to downstream and downwind ecosystems that then emit some of the N received as N2O and NO (x) . Emissions of NO (x) lead to the formation of tropospheric ozone, a greenhouse gas that can also harm crops directly. There are many opportunities to mitigate the impact of agricultural N on climate and the impact of climate on agricultural N. Some are available today; many need further research; and all await effective incentives to become adopted. Research needs can be grouped into four major categories: (1) an improved understanding of agricultural N cycle responses to changing climate; (2) a systems-level understanding of important crop and animal systems sufficient to identify key interactions and feedbacks; (3) the further development and testing of quantitative models capable of predicting N-climate interactions with confidence across a wide variety of crop-soil-climate combinations; and (4) socioecological research to better understand the incentives necessary to achieve meaningful deployment of realistic solutions. C1 [Robertson, G. Philip] Michigan State Univ, WK Kellogg Biol Stn, Dept Plant Soil & Microbial Sci, Hickory Corners, MI 49060 USA. [Robertson, G. Philip] Michigan State Univ, Great Lakes Bioenergy Res Ctr, Hickory Corners, MI USA. [Bruulsema, Tom W.] Int Plant Nutr Inst, Guelph, ON, Canada. [Gehl, Ron J.] NC State Univ, Dept Soil Sci, Raleigh, NC USA. [Kanter, David; Mauzerall, Denise L.] Princeton Univ, Woodrow Wilson Sch Publ & Int Affairs, Princeton, NJ 08544 USA. [Mauzerall, Denise L.] Princeton Univ, Dept Civil & Environm Engn, Princeton, NJ 08544 USA. [Rotz, C. Alan] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA USA. [Williams, Candiss O.] USDA, Charles E Kellogg Natl Soil Survey Lab, NRCS, Lincoln, NE USA. RP Robertson, GP (reprint author), Michigan State Univ, WK Kellogg Biol Stn, Dept Plant Soil & Microbial Sci, Hickory Corners, MI 49060 USA. EM robert30@msu.edu RI Mauzerall, Denise/I-5977-2013; OI Mauzerall, Denise/0000-0003-3479-1798; Robertson, G/0000-0001-9771-9895 FU NSF Research Coordination Network [DEB-0443439, DEB-1049744]; David and Lucille Packard Foundation; US Department of Energy [BER DE-FC02-07ER64494]; NSF Long-term Ecological Research Program FX This work resulted from a workshop supported by NSF Research Coordination Network awards DEB-0443439 and DEB-1049744 and by the David and Lucille Packard Foundation. We also acknowledge support for individual authors from the US Department of Energy (BER DE-FC02-07ER64494) and NSF Long-term Ecological Research Program (GPR). NR 183 TC 29 Z9 29 U1 4 U2 124 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 J9 BIOGEOCHEMISTRY JI Biogeochemistry PD JUL PY 2013 VL 114 IS 1-3 BP 41 EP 70 DI 10.1007/s10533-012-9802-4 PG 30 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 168PO UT WOS:000320718200004 ER PT J AU Porter, EM Bowman, WD Clark, CM Compton, JE Pardo, LH Soong, JL AF Porter, Ellen M. Bowman, William D. Clark, Christopher M. Compton, Jana E. Pardo, Linda H. Soong, Jenny L. TI Interactive effects of anthropogenic nitrogen enrichment and climate change on terrestrial and aquatic biodiversity SO BIOGEOCHEMISTRY LA English DT Article DE Climate change; Reactive nitrogen; Biodiversity ID GLOBAL ENVIRONMENTAL-CHANGES; ATMOSPHERIC CARBON-DIOXIDE; CHRONIC N FERTILIZATION; WESTERN UNITED-STATES; LOUISIANA SALT-MARSH; ELEVATED CO2; PLANT DIVERSITY; SPECIES RICHNESS; COMMUNITY STRUCTURE; SPARTINA-ALTERNIFLORA AB Biodiversity has been described as the diversity of life on earth within species, among species, and among ecosystems. The rate of biodiversity loss due to human activity in the last 50 years has been more rapid than at any other time in human history, and many of the drivers of biodiversity loss are increasing, including habitat loss, overexploitation, invasive species, climate change, and pollution, including pollution from reactive nitrogen (Nr). Of these stressors, climate change and Nr from anthropogenic activities are causing some of the most rapid changes. Climate change is causing warming trends that result in poleward and elevational range shifts of flora and fauna, and changes in phenology, particularly the earlier onset of spring events and migration, and lengthening of the growing season. Nitrogen (N) enrichment can enhance plant growth, but has been shown to favor, fast-growing, sometimes invasive, species over native species adapted to low N conditions. Although there have been only a few controlled studies on climate change and N interactions, inferences can be drawn from various field observations. For example, in arid ecosystems of southern California, elevated N deposition and changing precipitation patterns have promoted the conversion of native shrub communities to communities dominated by annual non-native grasses. Both empirical studies and modeling indicate that N and climate change can interact to drive losses in biodiversity greater than those caused by either stressor alone. Reducing inputs of anthropogenic Nr may be an effective mitigation strategy for protecting biodiversity in the face of climate change. C1 [Porter, Ellen M.] Natl Pk Serv, Air Resources Div, Denver, CO USA. [Bowman, William D.] Univ Colorado, Boulder, CO 80309 USA. [Clark, Christopher M.] US EPA, Washington, DC 20460 USA. [Compton, Jana E.] US EPA, Corvallis, OR USA. [Pardo, Linda H.] US Forest Serv, USDA, Burlington, VT USA. [Soong, Jenny L.] Colorado State Univ, Ft Collins, CO 80523 USA. RP Porter, EM (reprint author), Natl Pk Serv, Air Resources Div, Denver, CO USA. EM ellen_porter@nps.gov FU NSF Research Coordination Network [DEB-0443439, DEB-1049744]; David and Lucille Packard Foundation FX Many thanks to Eric Davidson and Emma Suddick for their assistance and help with planning and preparing this manuscript. Thanks to Nancy Dise, Edith Allen, and Rich Pouyat for providing thoughtful and helpful reviews of an older draft. This work resulted from a workshop supported by NSF Research Coordination Network awards DEB-0443439 and DEB-1049744 and by the David and Lucille Packard Foundation. NR 209 TC 20 Z9 20 U1 8 U2 185 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD JUL PY 2013 VL 114 IS 1-3 BP 93 EP 120 DI 10.1007/s10533-012-9803-3 PG 28 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 168PO UT WOS:000320718200006 ER PT J AU Fielding, DJ Trainor, E Zhang, MC AF Fielding, Dennis J. Trainor, Ellen Zhang, Mingchu TI Diet influences rates of carbon and nitrogen mineralization from decomposing grasshopper frass and cadavers SO BIOLOGY AND FERTILITY OF SOILS LA English DT Article DE Plant-herbivore interaction; Nitrogen mineralization; Nutrient cycling; Soil incubation; Herbivory; Insect defoliation; Alaska ID LITTER DECOMPOSITION; INSECT HERBIVORE; NUTRIENT FLOW; ISLE-ROYALE; OAK SAVANNA; PLANT; SOIL; ECOSYSTEM; DYNAMICS; ORTHOPTERA AB Insect herbivory can produce a pulse of mineral nitrogen (N) in soil from the decomposition of frass and cadavers. In this study, we examined how diet quality affects rates of N and carbon (C) mineralization from grasshopper frass and cadavers. Frass was collected from grasshoppers fed with natural or meridic diets which varied in N content. Frass was also collected from naturally foraging grasshoppers. Nitrogen concentration of frass was directly proportional to diet N, but N content of cadavers was not affected by diet. Incubations of soil plus frass were performed at constant soil moisture and temperature (15A degrees C) for 28 days, after which levels of mineral N (KCl extract) were determined. About 44 % of C and N from the cadavers were mineralized after the 28-day incubation. Carbon mineralization of frass was not affected by diet or frass N but varied considerably among different food treatments: from 15 to 46 % of the C in frass was released as carbon dioxide. Generally, frass with C/N ratio greater than 20 resulted in net immobilization of N. Results suggest that much of the N in grasshopper frass and cadavers is labile and rapidly available for plants, depending on the quality of food consumed by the grasshoppers. C1 [Fielding, Dennis J.; Trainor, Ellen] ARS, Subarctic Agr Res Unit, USDA, Fairbanks, AK 99709 USA. [Trainor, Ellen; Zhang, Mingchu] Univ Alaska Fairbanks, Sch Nat Resources & Agr Sci, Fairbanks, AK 99775 USA. RP Fielding, DJ (reprint author), ARS, Subarctic Agr Res Unit, USDA, 4169 Geist Rd, Fairbanks, AK 99709 USA. EM djfielding@alaska.edu NR 38 TC 0 Z9 0 U1 7 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0178-2762 J9 BIOL FERT SOILS JI Biol. Fertil. Soils PD JUL PY 2013 VL 49 IS 5 BP 537 EP 544 DI 10.1007/s00374-012-0702-5 PG 8 WC Soil Science SC Agriculture GA 178RR UT WOS:000321465600005 ER PT J AU Delwiche, SR Souza, EJ Kim, MS AF Delwiche, Stephen R. Souza, Edward J. Kim, Moon S. TI Limitations of single kernel near-infrared hyperspectral. imaging of soft wheat for milling quality SO BIOSYSTEMS ENGINEERING LA English DT Article ID PRINCIPAL COMPONENT ANALYSIS; DIFFUSE-REFLECTANCE SPECTRA; SOLVENT RETENTION CAPACITY; MULTIVARIATE DATA-ANALYSIS; TRITICUM-AESTIVUM; WHOLE GRAINS; HARDNESS; FLOUR; CLASSIFICATION; SPECTROSCOPY AB Soft wheat milling quality assessment typically begins in the later stages of wheat breeding programmes and continues after cultivar release in commercial milling and processing operations. Near-infrared (NW) hyperspectral (HS) image analysis, a technique that is gaining interest in food and pharmaceutical inspection research, was explored as an alternative procedure for milling quality evaluation. Three quality properties were studied, flour yield (the weight fraction of flour to whole grain), softness equivalent (a gauge of how easily flour is released from the kernel during break), and sucrose solvent retention capacity (SRC, related to arabinoxylans, which influence water absorption during dough mixing) because of their high degree of heritability and influence on soft wheat quality. NIR HS reflectance images (1000-1700 nm) of non-touching kernels were collected on more than 120 pure cultivars or advanced lines of soft red and white wheat. Five morphological properties (area, elliptical eccentricity and major and minor axis lengths, and ellipsoidal volume) and three spectral properties (principal component scores 1-3) were exhaustively examined in multiple linear regression models for each quality property and in nonparametric classification into low, medium, and high groups using texture properties (contrast, correlation, energy, and homogeneity) calculated from grey-level co-occurrence matrices of principal component scores images. Results indicated that softness equivalent exhibited the highest correlation with HS properties, while sucrose SRC had the lowest correlation. The combination of morphological and spectral properties produced better models than either property group alone. However, because of the inherent chemical and physical complexities of wheat, HS imaging will not be sufficient so as to replace actual pilot milling procedures. Published by Elsevier Ltd on behalf of IAgrE. C1 [Delwiche, Stephen R.] USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, BARC East, Beltsville, MD 20705 USA. [Souza, Edward J.] Ohio State Univ, Ohio Agr Res & Dev Ctr, USDA ARS, Soft Wheat Qual Lab, Wooster, OH 44691 USA. [Kim, Moon S.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Delwiche, SR (reprint author), USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, BARC East, Bldg 303, Beltsville, MD 20705 USA. EM stephen.delwiche@ars.usda.gov; moon.kim@ars.usda.gov NR 40 TC 7 Z9 8 U1 2 U2 34 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 EI 1537-5129 J9 BIOSYST ENG JI Biosyst. Eng. PD JUL PY 2013 VL 115 IS 3 BP 260 EP 273 DI 10.1016/j.biosystemseng.2013.03.015 PG 14 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA 173LI UT WOS:000321080600005 ER PT J AU Trabelsi, S Paz, AM Nelson, SO AF Trabelsi, Samir Paz, Ana M. Nelson, Stuart O. TI Microwave dielectric method for the rapid, non-destructive determination of bulk density and moisture content of peanut hull pellets SO BIOSYSTEMS ENGINEERING LA English DT Article ID GRANULAR-MATERIALS; PARTICULATE MATERIALS; WATER; FREQUENCIES; PERMITTIVITY; TEMPERATURE; SOLIDS; WHEAT; GRAIN AB A rapid dielectric-based method was used to non-destructively determine the moisture content and bulk density of peanut-hull pellets from free-space measurement of their dielectric properties at microwave frequencies. For moisture content determination, a permittivity-based function was used that permits moisture prediction independent of bulk density from measurement of the dielectric properties at microwave frequencies. For bulk density determination, an analytical expression was obtained from a representation of the dielectric properties in the complex plane. Results of moisture and density prediction from measurement of the dielectric properties are shown at 5, 10, and 15 GHz at 22 degrees C. Published by Elsevier Ltd on behalf of IAgrE. C1 [Trabelsi, Samir; Nelson, Stuart O.] ARS, USDA, Richard B Russell Agr Res Ctr, Qual & Safety Assessment Res Unit, Athens, GA 30605 USA. [Paz, Ana M.] Malardalen Univ, Dept Energy Technol, Vasteras, Sweden. RP Trabelsi, S (reprint author), ARS, USDA, Richard B Russell Agr Res Ctr, Qual & Safety Assessment Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM samir.trabelsi@ars.usda.gov OI Paz, Ana/0000-0001-7276-8447 NR 28 TC 12 Z9 12 U1 3 U2 28 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1537-5110 EI 1537-5129 J9 BIOSYST ENG JI Biosyst. Eng. PD JUL PY 2013 VL 115 IS 3 BP 332 EP 338 DI 10.1016/j.biosystemseng.2013.04.003 PG 7 WC Agricultural Engineering; Agriculture, Multidisciplinary SC Agriculture GA 173LI UT WOS:000321080600011 ER PT J AU Perry, RW Thill, RE AF Perry, Roger W. Thill, Ronald E. TI Comparison of snag densities among regeneration treatments in mixed pine-hardwood forests SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article ID CAVITY-NESTING BIRDS; BIG BROWN BATS; ROOST SELECTION; WESTERN OREGON; OLD-GROWTH; TREES; HABITAT; INTENSITY; LANDSCAPE; LONGEVITY AB Standing dead trees (snags) are an important component of forest ecosystems, providing foraging, nesting, and roosting substrate for a variety of vertebrates. We examined the effects of four forest regeneration treatments on residual snag density and compared those with densities found in unharvested, naturally regenerated forests (controls) during the second, fourth, and sixth year after timber harvest in mixed pine-hardwood forests of Arkansas and Oklahoma. Regeneration treatments were clearcut with snag creation, shelterwood, single-tree selection, and group selection. Density of large snags (>= 25.0 cm DBH) differed only during the sixth year after harvest, with shelterwoods having a lower density of large snags (1.0 snags/ha) than the control or group selection stands (4.0 and 4.2 snags/ha, respectively). Density of small snags (10.0-24.9 cm DBH) mirrored residual basal area, with controls and group-selection stands having the greatest snag densities. Creation of snags in clearcuts by injection with herbicides caused initial snag density in these areas to be greater than other treatments, but density in clearcuts declined sharply by 6 years after harvest. In the absence of snag creation, treatments such as shelterwoods that remove most trees may have snag densities below that required to address some management objectives without additional snag creation. C1 [Perry, Roger W.] US Forest Serv, USDA, Southern Res Stn, Hot Springs, AR 71902 USA. [Thill, Ronald E.] US Forest Serv, USDA, Southern Res Stn, Nacogdoches, TX USA. RP Perry, RW (reprint author), US Forest Serv, USDA, Southern Res Stn, POB 1270, Hot Springs, AR 71902 USA. EM rperry03@fs.fed.us NR 50 TC 0 Z9 1 U1 3 U2 24 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JUL PY 2013 VL 43 IS 7 BP 619 EP 626 DI 10.1139/cjfr-2013-0005 PG 8 WC Forestry SC Forestry GA 181WF UT WOS:000321699900003 ER PT J AU Schwarz, RS Evans, JD AF Schwarz, Ryan S. Evans, Jay D. TI Single and mixed-species trypanosome and microsporidia infections elicit distinct, ephemeral cellular and humoral immune responses in honey bees SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Apis mellifera; Endosymbiont; Host-parasite interaction; Innate immunity; Invertebrate; Protozoa ID NOSEMA-CERANAE MICROSPORIDIA; APIS-MELLIFERA; ANTIMICROBIAL PEPTIDES; GENE-EXPRESSION; DROSOPHILA-MELANOGASTER; MOLECULAR DIVERSITY; BOMBUS-TERRESTRIS; CRITHIDIA-BOMBI; COLONY COLLAPSE; HOST-DEFENSE AB Frequently encountered parasite species impart strong selective pressures on host immune system evolution and are more apt to concurrently infect the same host, yet molecular impacts in light of this are often overlooked. We have contrasted immune responses in honey bees to two common eukaryotic endoparasites by establishing single and mixed-species infections using the long-associated parasite Crithidia mellificae and the emergent parasite Nosema ceranae. Quantitative polymerase chain reaction was used to screen host immune gene expression at 9 time points post inoculation. Systemic responses in abdomens during early stages of parasite establishment revealed conserved receptor (Down syndrome cell adhesion molecule, Dscam and nimrod C1, nimC1), signaling (MyD88 and Imd) and antimicrobial peptide (AMP) effector (Defensin 2) responses. Late, established infections were distinct with a refined 2 AMP response to C mellificae that contrasted starkly with a 5 AMP response to N. ceranae. Mixed species infections induced a moderate 3 AMPs. Transcription in gut tissues highlighted important local roles for Dscam toward both parasites and Imd signaling toward N. ceranae. At both systemic and local levels Dscam, MyD88 and Imd transcription was consistently correlated based on clustering analysis. Significant gene suppression occurred in two cases from midgut to ileum tissue: Dscam was lowered during mixed infections compared to N. ceranae infections and both C mellificae and mixed infections had reduced nimC1 transcription compared to uninfected controls. We show that honey bees rapidly mount complex immune responses to both Nosema and Crithidia that are dynamic over time and that mixed-species infections significantly alter local and systemic immune gene transcription. Published by Elsevier Ltd. C1 [Schwarz, Ryan S.; Evans, Jay D.] USDA ARS, Bee Res Lab, Beltsville, MD 20705 USA. RP Schwarz, RS (reprint author), USDA ARS, Bee Res Lab, BARC East Bldg 306,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM schwarz.ryan@gmail.com; evans@ars.usda.gov RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 NR 65 TC 28 Z9 30 U1 3 U2 56 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD JUL-AUG PY 2013 VL 40 IS 3-4 BP 300 EP 310 DI 10.1016/j.dci.2013.03.010 PG 11 WC Immunology; Zoology SC Immunology; Zoology GA 173PJ UT WOS:000321091100009 PM 23529010 ER PT J AU Tanner, CC Howard-Williams, C Tomer, MD Lowrance, R AF Tanner, Chris C. Howard-Williams, Clive Tomer, Mark D. Lowrance, Richard TI Bringing together science and policy to protect and enhance wetland ecosystem services in agricultural landscapes SO ECOLOGICAL ENGINEERING LA English DT Editorial Material C1 [Tanner, Chris C.] Natl Inst Water & Atmospher Res, Hamilton, New Zealand. [Howard-Williams, Clive] Natl Inst Water & Atmospher Res, Christchurch, New Zealand. [Tomer, Mark D.] USDA ARS, SE Watershed Res Lab, Ames, IA USA. [Lowrance, Richard] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. RP Tanner, CC (reprint author), Natl Inst Water & Atmospher Res, Hamilton, New Zealand. EM c.tanner@niwa.co.nz; clive.howard-williams@niwa.co.nz; Mark.Tomer@ARS.USDA.GOV; Richard.Lowrance@ARS.USDA.GOV NR 19 TC 7 Z9 7 U1 5 U2 55 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 J9 ECOL ENG JI Ecol. Eng. PD JUL PY 2013 VL 56 SI SI BP 1 EP 4 DI 10.1016/j.ecoleng.2013.03.008 PG 4 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA 171DH UT WOS:000320904800001 ER PT J AU Tomer, MD Crumpton, WG Bingner, RL Kostel, JA James, DE AF Tomer, M. D. Crumpton, W. G. Bingner, R. L. Kostel, J. A. James, D. E. TI Estimating nitrate load reductions from placing constructed wetlands in a HUC-12 watershed using LiDAR data SO ECOLOGICAL ENGINEERING LA English DT Article DE Light detection and ranging (LiDAR); Nutrient-removal wetlands; Nitrate; Precision conservation; AnnAGNPS ID MISSISSIPPI RIVER-BASIN; TILE DRAINAGE; AGRICULTURAL WATERSHEDS; CONSERVATION PRACTICES; NITROGEN; QUALITY; EXPORT; ILLINOIS; USA; PHOSPHORUS AB Mitigating Gulf of Mexico hypoxia will require practices to reduce nitrate losses from tile drainage throughout the upper Mississippi River basin. Wetlands are a key practice to accomplish this via denitrification, but locations of feasible wetland sites will need to be determined on a watershed specific basis. This study's objective was to demonstrate that LiDAR topographic data can be used to identify feasible wetland locations in a 6500 ha watershed. in northern Illinois, and then estimate the impact on watershed nitrate loads from wetlands hypothetically constructed at those locations. The evaluation resulted in the identification of eleven sites where wetlands could intercept tile drainage from 30% of the watershed. The USDA AnnAGNPS model was used to estimate nitrate loads delivered to candidate wetlands during a 30-year period of simulated climate. The model results were consistent with discharge and nitrate loading regimes in the region. Nitrate reduction by the wetlands was estimated using a published regression model. The wetlands could reduce average watershed nitrate loads by 11-13%, a significant reduction from treating just 30% of the watershed with a single practice. Results showed a wide variation in N-removal performance among wetlands, due to varying contributing-to-wetland area ratios, and varying land uses that affected discharge and N loads among locations. A large wetland installed at the watershed outlet could achieve an estimated N-load reduction of 35% under relaxed siting criteria, but would require the added expense of roadway reconstruction. These conclusions are relevant for planning watershed conservation efforts and establishment of nutrient trading schemes. Published by Elsevier B.V. C1 [Tomer, M. D.; James, D. E.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Crumpton, W. G.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA USA. [Bingner, R. L.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. [Kostel, J. A.] Wetlands Initiat, Chicago, IL USA. RP Tomer, MD (reprint author), USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. EM mark.tomer@gmail.com FU US Environmental Protection Agency FX This research was funded in part by the US Environmental Protection Agency under a 2009 Targeted Watershed Grant. Thanks to Jared Bean for assisting with GIS processing of the LiDAR data. NR 42 TC 18 Z9 19 U1 1 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-8574 EI 1872-6992 J9 ECOL ENG JI Ecol. Eng. PD JUL PY 2013 VL 56 SI SI BP 69 EP 78 DI 10.1016/j.ecoleng.2012.04.040 PG 10 WC Ecology; Engineering, Environmental; Environmental Sciences SC Environmental Sciences & Ecology; Engineering GA 171DH UT WOS:000320904800007 ER PT J AU Clark, CM Morefield, PE Gilliam, FS Pardo, LH AF Clark, Christopher M. Morefield, Philip E. Gilliam, Frank S. Pardo, Linda H. TI Estimated losses of plant biodiversity in the United States from historical N deposition (1985-2010) SO ECOLOGY LA English DT Article DE biodiversity; critical loads; ecoregion; herbaceous; nitrogen deposition ID NITROGEN CRITICAL LOADS; SPECIES RICHNESS; GRASSLANDS; DIVERSITY; RESTORATION; COMMUNITIES; CESSATION; EUROPE; LEVEL AB Although nitrogen (N) deposition is a significant threat to herbaceous plant biodiversity worldwide, it is not a new stressor for many developed regions. Only recently has it become possible to estimate historical impacts nationally for the United States. We used 26 years (1985-2010) of deposition data, with ecosystem-specific functional responses from local field experiments and a national critical loads (CL) database, to generate scenario-based estimates of herbaceous species loss. Here we show that, in scenarios using the low end of the CL range, N deposition exceeded critical loads over 0.38, 6.5, 13.1, 88.6, and 222.1 million ha for the Mediterranean California, North American Desert, Northwestern Forested Mountains, Great Plains, and Eastern Forest ecoregions, respectively, with corresponding species losses ranging from <1% to 30%. When we ran scenarios assuming ecosystems were less sensitive (using a common CL of 10 kgha(-1)yr(-1), and the high end of the CL range) minimal losses were estimated. The large range in projected impacts among scenarios implies uncertainty as to whether current critical loads provide protection to terrestrial plant biodiversity nationally and urge greater research in refining critical loads for U.S. ecosystems. C1 [Clark, Christopher M.; Morefield, Philip E.] US EPA, Off Res & Dev, Natl Ctr Environm Assessment, Washington, DC 20460 USA. [Gilliam, Frank S.] Marshall Univ, Dept Biol Sci, Huntington, WV 25755 USA. [Pardo, Linda H.] US Forest Serv, USDA, No Res Stn, Burlington, VT 05405 USA. RP Clark, CM (reprint author), US EPA, Off Res & Dev, Natl Ctr Environm Assessment, 1200 Penn Ave,NW,Mail Code 8601-P, Washington, DC 20460 USA. EM clark.christopher@epa.gov NR 30 TC 22 Z9 22 U1 5 U2 97 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 J9 ECOLOGY JI Ecology PD JUL PY 2013 VL 94 IS 7 BP 1441 EP 1448 DI 10.1890/12-2016.1 PG 8 WC Ecology SC Environmental Sciences & Ecology GA 180SY UT WOS:000321618400001 PM 23951703 ER PT J AU Flitcroft, R Burnett, K Christiansen, K AF Flitcroft, Rebecca Burnett, Kelly Christiansen, Kelly TI A Simple Model that Identifies Potential Effects of Sea-Level Rise on Estuarine and Estuary-Ecotone Habitat Locations for Salmonids in Oregon, USA SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Salmonids; Digital elevation models; LiDAR; Sea-level rise; Estuary; Habitat ID CLIMATE-CHANGE; RIVER ESTUARY; PACIFIC-NORTHWEST; ONCORHYNCHUS-TSHAWYTSCHA; SALT-MARSH; RESILIENCE; SURVIVAL; TEMPERATURE; MANAGEMENT; RESIDENCY AB Diadromous aquatic species that cross a diverse range of habitats (including marine, estuarine, and freshwater) face different effects of climate change in each environment. One such group of species is the anadromous Pacific salmon (Oncorhynchus spp.). Studies of the potential effects of climate change on salmonids have focused on both marine and freshwater environments. Access to a variety of estuarine habitat has been shown to enhance juvenile life-history diversity, thereby contributing to the resilience of many salmonid species. Our study is focused on the effect of sea-level rise on the availability, complexity, and distribution of estuarine, and low-freshwater habitat for Chinook salmon (Oncorhynchus tshawytscha), steelhead (anadromous O. mykiss), and coho salmon (O. kisutch) along the Oregon Coast under future climate change scenarios. Using LiDAR, we modeled the geomorphologies of five Oregon estuaries and estimated a contour associated with the current mean high tide. Contour intervals at 1- and 2-m increments above the current mean high tide were generated, and changes in the estuary morphology were assessed. Because our analysis relied on digital data, we compared three types of digital data in one estuary to assess the utility of different data sets in predicting the changes in estuary shape. For each salmonid species, changes in the amount and complexity of estuarine edge habitats varied by estuary. The simple modeling approach we applied can also be used to identify areas that may be most amenable to pre-emptive restoration actions to mitigate or enhance salmonid habitat under future climatic conditions. C1 [Flitcroft, Rebecca; Burnett, Kelly; Christiansen, Kelly] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. RP Flitcroft, R (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM becky.flitcroft@oregonstate.edu FU USDA Forest Service, PNW Research Station FX The analysis presented in this study was funded by the USDA Forest Service, PNW Research Station. We would like to thank the Oregon Department of Fish and Wildlife for their work documenting salmonid habitat throughout Oregon. We would also like to thank the LiDAR Consortium that organized and flew the Oregon Coastal Province. We thank the Siuslaw National Forest for making the low-tide LiDAR of the Salmon River Estuary available to us for this work. Additionally, we thank Deborah Reusser of the USGS for her thoughtful comments regarding the effects of SLR on estuarine habitats. The comments from four anonymous reviewers significantly improved the manuscript. NR 59 TC 1 Z9 1 U1 1 U2 40 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X J9 ENVIRON MANAGE JI Environ. Manage. PD JUL PY 2013 VL 52 IS 1 BP 196 EP 208 DI 10.1007/s00267-013-0074-0 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA 173EY UT WOS:000321062100015 PM 23689791 ER PT J AU Nowak, DJ Greenfield, EJ Hoehn, RE Lapoint, E AF Nowak, David J. Greenfield, Eric J. Hoehn, Robert E. Lapoint, Elizabeth TI Carbon storage and sequestration by trees in urban and community areas of the United States SO ENVIRONMENTAL POLLUTION LA English DT Article DE Ecosystem services; Global climate change; Urban forestry; Tree cover; Forest inventory ID FOREST RESOURCE; EMISSIONS; IMPACTS; STOCKS; SCALE; COVER; CITY AB Carbon storage and sequestration by urban trees in the United States was quantified to assess the magnitude and role of urban forests in relation to climate change. Urban tree field data from 28 cities and 6 states were used to determine the average carbon density per unit of tree cover. These data were applied to statewide urban tree cover measurements to determine total urban forest carbon storage and annual sequestration by state and nationally. Urban whole tree carbon storage densities average 7.69 kg C m(-2) of tree cover and sequestration densities average 0.28 kg C m(-2) of tree cover per year. Total tree carbon storage in U.S. urban areas (c. 2005) is estimated at 643 million tonnes ($50.5 billion value; 95% CI = 597 million and 690 million tonnes) and annual sequestration is estimated at 25.6 million tonnes ($2.0 billion value; 95% CI = 23.7 million to 27.4 million tonnes). Published by Elsevier Ltd. C1 [Nowak, David J.; Greenfield, Eric J.; Hoehn, Robert E.] US Forest Serv, USDA, No Res Stn, Syracuse, NY 13210 USA. [Lapoint, Elizabeth] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. RP Nowak, DJ (reprint author), US Forest Serv, USDA, No Res Stn, Syracuse, NY 13210 USA. EM dnowak@fs.fed.us; ejgreenfield@fs.fed.us; rhoehn@fs.fed.us; elapoint@fs.fed.us FU State and Private Forestry's Urban and Community Forestry Program; National Science Foundation (NSF) through the Baltimore Ecosystem Study-Long Term Ecological Research (BES-LTER) [DEB-0423476, BCS-0948952]; Syracuse Urban Long-term Research Area Exploratory Award (ULTRA-Ex) FX Funding for this project was provided, in part, by the USDA Forest Service's RPA Assessment Staff and State and Private Forestry's Urban and Community Forestry Program and the National Science Foundation (NSF grants DEB-0423476 and BCS-0948952) through the Baltimore Ecosystem Study-Long Term Ecological Research (BES-LTER) and the Syracuse Urban Long-term Research Area Exploratory Award (ULTRA-Ex). The authors thank the numerous people and organizations involved with field data collection. The authors also thank John Stanovick for his statistical assistance and review, and Linda Heath, Mark Flugge, Nicholas Devonshire and Jennifer Jenkins for their comments on a draft manuscript. NR 52 TC 66 Z9 72 U1 12 U2 175 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUL PY 2013 VL 178 BP 229 EP 236 DI 10.1016/j.envpol.2013.03.019 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 165MJ UT WOS:000320487700031 PM 23583943 ER PT J AU Nowak, DJ Hirabayashi, S Bodine, A Hoehn, R AF Nowak, David J. Hirabayashi, Satoshi Bodine, Allison Hoehn, Robert TI Modeled PM2.5 removal by trees in ten US cities and associated health effects SO ENVIRONMENTAL POLLUTION LA English DT Article DE Urban forests; Air pollution removal; Particulate matter; Mortality; Human health ID PARTICULATE AIR-POLLUTION; POSTNEONATAL INFANT-MORTALITY; LONG-TERM EXPOSURE; HOSPITAL ADMISSIONS; FINE PARTICLES; RESPIRATORY SYMPTOMS; MYOCARDIAL-INFARCTION; EMERGENCY ADMISSIONS; AMERICAN CHILDREN; DRY DEPOSITION AB Urban particulate air pollution is a serious health issue. Trees within cities can remove fine particles from the atmosphere and consequently improve air quality and human health. Tree effects on PM2.5 concentrations and human health are modeled for 10 U.S. cities. The total amount of PM2.5 removed annually by trees varied from 4.7 tonnes in Syracuse to 64.5 tonnes in Atlanta, with annual values varying from $1.1 million in Syracuse to $60.1 million in New York City. Most of these values were from the effects of reducing human mortality. Mortality reductions were typically around 1 person yr(-1) per city, but were as high as 7.6 people yr(-1) in New York City. Average annual percent air quality improvement ranged between 0.05% in San Francisco and 0.24% in Atlanta. Understanding the impact of urban trees on air quality can lead to improved urban forest management strategies to sustain human health in cities. Published by Elsevier Ltd. C1 [Nowak, David J.; Hoehn, Robert] SUNY ESF, US Forest Serv, USDA, Moon Lib 5, Syracuse, NY 13210 USA. [Hirabayashi, Satoshi; Bodine, Allison] SUNY ESF, Davey Inst, Moon Lib 5, Syracuse, NY 13210 USA. RP Nowak, DJ (reprint author), SUNY ESF, US Forest Serv, USDA, Moon Lib 5, Syracuse, NY 13210 USA. EM dnowak@fs.fed.us FU National Science Foundation (NSF) through the Baltimore Ecosystem Study-Long Term Ecological Research (BES-LTER) [DEB-0423476, BCS-0948952]; Syracuse Urban Long-term Research Area Exploratory Award (ULTRA-Ex) FX Funding for this project was provided, in part, by the National Science Foundation (NSF grants DEB-0423476 and BCS-0948952) through the Baltimore Ecosystem Study-Long Term Ecological Research (BES-LTER) and the Syracuse Urban Long-term Research Area Exploratory Award (ULTRA-Ex). The authors thank Jin Huang, Ryan Stapler and Alex Foreste for their comments on a draft manuscript. NR 66 TC 60 Z9 87 U1 9 U2 136 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 J9 ENVIRON POLLUT JI Environ. Pollut. PD JUL PY 2013 VL 178 BP 395 EP 402 DI 10.1016/j.envpol.2013.03.050 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 165MJ UT WOS:000320487700052 PM 23624337 ER PT J AU Crutcher, FK Parich, A Schuhmacher, R Mukherjee, PK Zeilinger, S Kenerley, CM AF Crutcher, Frankie K. Parich, Alexandra Schuhmacher, Rainer Mukherjee, Prasun K. Zeilinger, Susanne Kenerley, Charles M. TI A putative terpene cyclase, vir4, is responsible for the biosynthesis of volatile terpene compounds in the biocontrol fungus Trichoderma virens SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Trichoderma; Natural products; Terpenes; Vir4; GC/MS ID SECONDARY METABOLITES; ASPERGILLUS-FUMIGATUS; ANTIBIOTIC PRODUCTION; FILAMENTOUS FUNGI; ORGANIC-COMPOUNDS; GENE; EXPRESSION; SESQUITERPENES; PROTEIN; MORPHOGENESIS AB A putative terpene cyclase vir4, which is a member of a secondary metabolite cluster, has been deleted in Trichoderma virens to determine its function. The deletion mutants were compared for volatile production with the wild-type as well as two other Trichoderma spp. This gene cluster was originally predicted to function in the synthesis of viridin and viridiol. However, the experimental evidence demonstrates that this gene cluster is involved in the synthesis of volatile terpene compounds. The entire vir4-containing gene cluster is absent in two other species of Trichoderma, T. atroviride and T. reesei. Neither of these two species synthesizes volatile terpenes associated with this cluster in T. virens. We have thus identified a novel class of volatile fungal sesquiterpenes as well as the gene cluster involved in their biosynthesis. (C) 2013 Elsevier Inc. All rights reserved. C1 [Kenerley, Charles M.] Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. [Crutcher, Frankie K.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Parich, Alexandra; Schuhmacher, Rainer] Univ Nat Resources & Appl Life Sci, Ctr Analyt Chem, Dept Agrobiotechnol IFATulln, A-3430 Tulln, Austria. [Mukherjee, Prasun K.] Bhabha Atom Res Ctr, Mumbai 400085, Maharashtra, India. [Zeilinger, Susanne] Vienna Univ Technol, Inst Chem Engn, Res Area Biotechnol & Microbiol, A-1060 Vienna, Austria. RP Kenerley, CM (reprint author), Texas A&M Univ, Dept Plant Pathol & Microbiol, College Stn, TX 77843 USA. EM c-kenerley@tamu.edu OI Zeilinger, susanne/0000-0003-3112-0948 FU National Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [2008-35319-04470]; Texas Department of Agriculture; US-Israel Binational Agricultural Research and Development Fund [TB-8031-08]; USDA National Needs Fellowship FX We thank Drs. Robert Stipanovic and Lorraine Puckhaber (USDA-ARS-Southern Plains Agricultural Research Center) for secondary metabolite analysis by HPLC. Bernard Kluger and Christoph Konlechner are also greatly acknowledged for their technical assistance in volatile determination. This work was supported by the National Research Initiative Competitive Grants Program (Grant no. 2008-35319-04470) from the USDA National Institute of Food and Agriculture, and the Texas Department of Agriculture and the US-Israel Binational Agricultural Research and Development Fund (Grant no. TB-8031-08). Three years of fellowship support was also provided by a USDA National Needs Fellowship. NR 46 TC 13 Z9 13 U1 3 U2 43 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUL PY 2013 VL 56 BP 67 EP 77 DI 10.1016/j.fgb.2013.05.003 PG 11 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 179PU UT WOS:000321534600008 PM 23707931 ER PT J AU Kasson, MT O'Donnell, K Rooney, AP Sink, S Ploetz, RC Ploetz, JN Konkol, JL Carrillo, D Freeman, S Mendel, Z Smith, JA Black, AW Hulcr, J Bateman, C Stefkova, K Campbell, PR Geering, ADW Dann, EK Eskalen, A Mohotti, K Short, DPG Aoki, T Fenstermacher, KA Davis, DD Geiser, DM AF Kasson, Matthew T. O'Donnell, Kerry Rooney, Alejandro P. Sink, Stacy Ploetz, Randy C. Ploetz, Jill N. Konkol, Joshua L. Carrillo, Daniel Freeman, Stanley Mendel, Zvi Smith, Jason A. Black, Adam W. Hulcr, Jiri Bateman, Craig Stefkova, Kristyna Campbell, Paul R. Geering, Andrew D. W. Dann, Elizabeth K. Eskalen, Akif Mohotti, Keerthi Short, Dylan P. G. Aoki, Takayuki Fenstermacher, Kristi A. Davis, Donald D. Geiser, David M. TI An inordinate fondness for Fusarium: Phylogenetic diversity of fusaria cultivated by ambrosia beetles in the genus Euwallacea on avocado and other plant hosts SO FUNGAL GENETICS AND BIOLOGY LA English DT Article DE Ambrosia fungi; Divergence dating; Evolution; Mutualism; Mycangia; Symbiosis ID SOLANI SPECIES COMPLEX; BARK BEETLES; FUNGAL ENDOPHYTES; UNITED-STATES; SP-NOV; CURCULIONIDAE; SCOLYTINAE; EVOLUTION; RECOMBINATION; AGRICULTURE AB Ambrosia beetle fungiculture represents one of the most ecologically and evolutionarily successful symbioses, as evidenced by the 11 independent origins and 3500 species of ambrosia beetles. Here we document the evolution of a clade within Fusarium associated with ambrosia beetles in the genus Euwallacea (Coleoptera: Scolytinae). Ambrosia Fusarium Clade (AFC) symbionts are unusual in that some are plant pathogens that cause significant damage in naive natural and cultivated ecosystems, and currently threaten avocado production in the United States, Israel and Australia. Most AFC fusaria produce unusual clavate macroconidia that serve as a putative food source for their insect mutualists. AFC symbionts were abundant in the heads of four Euwallacea spp., which suggests that they are transported within and from the natal gallery in mandibular mycangia. In a four-locus phylogenetic analysis, the AFC was resolved in a strongly supported monophyletic group within the previously described Cade 3 of the Fusarium solani species complex (FSSC). Divergence-time estimates place the origin of the AFC in the early Miocene similar to 21.2 Mya, which coincides with the hypothesized adaptive radiation of the Xyleborini. Two strongly supported clades within the AFC (Clades A and B) were identified that include nine species lineages associated with ambrosia beetles, eight with Euwallacea spp. and one reportedly with Xyleborus ferrugineus, and two lineages with no known beetle association. More derived lineages within the AFC showed fixation of the clavate (club-shaped) macroconidial trait, while basal lineages showed a mix of clavate and more typical fusiform macroconidia. AFC lineages consisted mostly of genetically identical individuals associated with specific insect hosts in defined geographic locations, with at least three interspecific hybridization events inferred based on discordant placement in individual gene genealogies and detection of recombinant loci. Overall, these data are consistent with a strong evolutionary trend toward obligate symbiosis coupled with secondary contact and interspecific hybridization. (C) 2013 Elsevier Inc. All rights reserved. C1 [Kasson, Matthew T.; Fenstermacher, Kristi A.; Davis, Donald D.; Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [O'Donnell, Kerry; Sink, Stacy] USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Rooney, Alejandro P.] USDA ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Ploetz, Randy C.; Ploetz, Jill N.; Konkol, Joshua L.; Carrillo, Daniel] Univ Florida, IFAS, Ctr Trop Res & Educ, Homestead, FL 33031 USA. [Freeman, Stanley; Mendel, Zvi] Agr Res Org, Volcani Ctr, Inst Plant Protect, IL-50250 Bet Dagan, Israel. [Smith, Jason A.; Black, Adam W.; Hulcr, Jiri; Bateman, Craig] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Stefkova, Kristyna] Univ South Bohemia, Ceske Budejovice 37005, Czech Republic. [Campbell, Paul R.; Geering, Andrew D. W.; Dann, Elizabeth K.] Univ Queensland, Queensland Dept Agr Fisheries & Forestry, Dutton Pk, Qld 4102, Australia. [Campbell, Paul R.; Geering, Andrew D. W.; Dann, Elizabeth K.] Univ Queensland, Queensland Alliance Agr & Food Innovat, Dutton Pk, Qld 4102, Australia. [Eskalen, Akif] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. [Mohotti, Keerthi] Tea Res Inst Sri Lanka, Entomol & Nematol Div, Talawakelle 22100, Sri Lanka. [Short, Dylan P. G.] Univ Calif Davis, Dept Plant Pathol, Salinas, CA 93905 USA. [Aoki, Takayuki] Natl Inst Agrobiol Sci, Genet Resources Ctr, Tsukuba, Ibaraki 3058602, Japan. RP Kasson, MT (reprint author), Virginia Tech Univ, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM mkasson@vt.edu RI Geering, Andrew/A-7912-2011; Geiser, David/J-9950-2013; Dann, Elizabeth/A-7291-2011 OI Dann, Elizabeth/0000-0002-0239-8679 FU USDA Forest Service Forest Health Technology Enterprise Team (FHTET), Morgantown, WV; Czech Ministry of Education, Youth and Sports [LH 12098 KONTAKT II CR]; Norwegian Research Council; AFRI from the USDA NIFA [2010-65110-20488] FX The authors thank the Hofshi Foundation for organizing the Invasive Ambrosia Beetle Conference in August 2012, which greatly facilitated this collaboration. M.T.K. and D.D.D. thank the USDA Forest Service Forest Health Technology Enterprise Team (FHTET), Morgantown, WV, for funding, and Suzanne Slack and Jean Juba for their assistance in the lab. KS. was funded from the Czech Ministry of Education, Youth and Sports Project no. LH 12098 KONTAKT II CR - USA. C.B. and J.H. were funded from a Cooperative Agreement with the USDA Forest Service, Forest Health Section, and by the Norwegian Research Council. D.P.G.S. was supported by AFRI grant no. 2010-65110-20488 from the USDA NIFA. We are pleased to thank Nathane Orwig for running sequences in the NCAUR DNA Core Facility. A.G., P.C. and E.D. thank Horticulture Australia Limited and Avocados Australia Limited for funding. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. NR 49 TC 32 Z9 33 U1 7 U2 232 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1087-1845 J9 FUNGAL GENET BIOL JI Fungal Genet. Biol. PD JUL PY 2013 VL 56 BP 147 EP 157 DI 10.1016/j.fgb.2013.04.004 PG 11 WC Genetics & Heredity; Mycology SC Genetics & Heredity; Mycology GA 179PU UT WOS:000321534600016 PM 23608321 ER PT J AU Fisher, JO Birch, LL Zhang, J Grusak, MA Hughes, SO AF Fisher, J. O. Birch, L. L. Zhang, J. Grusak, M. A. Hughes, S. O. TI External influences on children's self- served portions at meals SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE portion size; spoon size; children; appetite; feeding styles; family style meals ID ENERGY-INTAKE; CONSUMPTION VOLUME; FEEDING PRACTICES; AFRICAN-AMERICAN; SIZE; WEIGHT; STYLES; BOWLS; AGE AB OBJECTIVE: Large portions promote intake among children, but little is known about the external influences of the eating environment on children's self-selected portion sizes. This research experimentally tested effects of the amount of entree available and serving spoon size on children's self-served entree portions and intakes at dinner meals. A secondary objective was to identify child and family predictors of self-served entree portion sizes. DESIGN: A 2 x 2 within-subjects design was used, in which the amount of a pasta entree available for self-serving (275 vs 550 g) and the serving spoon size (teaspoon vs tablespoon) were systematically varied. The serving bowl size and portion sizes of all other foods offered were held constant across conditions. Conditions were spaced 1 week apart and randomly assigned. Weighed self-served entree portions and food intakes as well as demographics, maternal feeding styles and child/maternal anthropometrics were measured. SUBJECTS: Participants were 60 ethnically diverse children aged 4-6 years and their mothers. RESULTS: Mixed models revealed that children served themselves 40% more entree when the amount available was doubled (P < 0.0001) and 13% more when the serving spoon size was tripled (P < 0.05). Serving spoon size and the amount of entree available indirectly influenced children's intake, with larger self-served portion sizes related to greater entree intakes (P < 0.0001). Greater self-served portions and energy intakes at the meal were seen among those children whose mothers reported indulgent or authoritarian feeding styles (P < 0.001). CONCLUSION: Children's self-served portion sizes at meals are influenced by size-related facets of the eating environment and reflect maternal feeding styles. C1 [Fisher, J. O.] Temple Univ, Dept Publ Hlth, Ctr Obes Res & Educ, Philadelphia, PA 19140 USA. [Birch, L. L.] Penn State Univ, Ctr Childhood Obes Res, Dept Human Dev & Family Studies, Philadelphia, PA USA. [Zhang, J.] AdvanceMed Corp, Grove City, OH USA. [Grusak, M. A.; Hughes, S. O.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Fisher, JO (reprint author), Temple Univ, Dept Publ Hlth, Ctr Obes Res & Educ, 3223 N Broad, Philadelphia, PA 19140 USA. EM jofisher@temple.edu FU USDA NRI [2006-55215-16694]; USDA CRIS FX We would like to express gratitude to the research staff who collected these data and to the families who participated in the research. This work was supported by USDA NRI 2006-55215-16694 and USDA CRIS funds to the USDA/ARS Children's Nutrition Research Center, Houston, TX, USA. NR 32 TC 18 Z9 18 U1 3 U2 20 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 EI 1476-5497 J9 INT J OBESITY JI Int. J. Obes. PD JUL PY 2013 VL 37 IS 7 BP 954 EP 960 DI 10.1038/ijo.2012.216 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA 180BJ UT WOS:000321567500011 PM 23295501 ER PT J AU Bublin, M Kostadinova, M Radauer, C Hafner, C Szepfalusi, Z Varga, EM Maleki, SJ Hoffmann-Sommergruber, K Breiteneder, H AF Bublin, Merima Kostadinova, Maria Radauer, Christian Hafner, Christine Szepfalusi, Zsolt Varga, Eva-Maria Maleki, Soheila J. Hoffmann-Sommergruber, Karin Breiteneder, Heimo TI IgE cross-reactivity between the major peanut allergen Ara h 2 and the nonhomologous allergens Ara h 1 and Ara h 3 SO JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY LA English DT Article DE Allergens; food allergy; IgE cross-reactivity; peanut allergy ID IMMUNOGLOBULIN-E-BINDING; MICROARRAY IMMUNOASSAY; MUTATIONAL ANALYSIS; CRYSTAL-STRUCTURE; ARACHIS-HYPOGAEA; EPITOPES; PROTEIN; SEVERITY; CHILDREN; IDENTIFICATION AB Background: Ara h 1, a vicilin; Ara h 2, a 2S albumin; and Ara h 3, a legumin, are major peanut allergens. Ara h 2 is an important predictor of clinical reactivity to peanut, but cosensitization to all 3 allergens is correlated with the severity of patients' symptoms. Objective: We investigated whether cosensitization to these 3 allergens is caused by IgE cross-reactivity, despite the fact that they do not display obvious structural or sequence similarities. Methods: IgE cross-inhibitions were performed with purified Ara h 1, Ara h 2, and Ara h 3 and IgG-depleted sera from 10 patients with peanut allergy. After an in silico search for similar peptides, IgE ELISA inhibition assays with synthetic peptides were performed. Results: Ara h 2 inhibited IgE binding to Ara h 1 (average, 86% +/- 13%) and Ara h 3 (average, 96% +/- 6%). IgE binding to Ara h 2 was inhibited by Ara h 1 by 78% +/- 15% and by Ara h 3 by 80% +/- 6%. A subsequent sequence comparison showed that these nonhomologous allergens contained several similar surface-exposed peptides. IgE binding to Ara h 2-derived peptides was completely inhibited by Ara h 1 and Ara h 3. A mixture of these peptides reduced IgE binding to Ara h 1 and Ara h 3 by 20% to 60% and to Ara h 2 by 49% to 89%. Conclusion: Occurrence of similar sequences in the 3 major peanut allergens accounts for the high extent of cross-reactivity among them. C1 [Bublin, Merima; Kostadinova, Maria; Radauer, Christian; Hoffmann-Sommergruber, Karin; Breiteneder, Heimo] Med Univ Vienna, Dept Pathophysiol & Allergy Res, A-1090 Vienna, Austria. [Hafner, Christine] Karl Landsteiner Inst Dermatol Res, St Polten, Austria. [Szepfalusi, Zsolt] Med Univ Vienna, Dept Paediat & Adolescent Med, A-1090 Vienna, Austria. [Varga, Eva-Maria] Med Univ Graz, Dept Paediat, Resp & Allerg Dis Div, Graz, Austria. [Maleki, Soheila J.] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. RP Bublin, M (reprint author), Med Univ Vienna, Dept Pathophysiol & Allergy Res, Waehringer Guertel 18-20, A-1090 Vienna, Austria. EM merima.bublin@meduniwien.ac.at OI Radauer, Christian/0000-0001-9920-4449 FU BRIDGE Grant from the Austrian Research Promotion Agency (FFG) [820127]; Roche; Bristol-Myers Squibb; Phadia Austria; Thermo Fisher Diagnostics AB; SNF FX Supported by BRIDGE Grant 820127 from the Austrian Research Promotion Agency (FFG; to H.B.).; C. Hafner has received lecture fees from Roche, Bristol-Myers Squibb, and Phadia Austria. K. Hoffmann-Sommergruber has received consulting fees from Thermo Fisher Diagnostics AB, has received fees for participation in a reviewer board from SNF, has received royalties from CRD Press for coediting Managing Allergens in Food (2007), and has received travel support for EAACI Meetings in relation to the Interest Group for Food Allergy. The rest of the authors declare that they have no relevant conflicts of interest. NR 44 TC 27 Z9 28 U1 0 U2 24 PU MOSBY-ELSEVIER PI NEW YORK PA 360 PARK AVENUE SOUTH, NEW YORK, NY 10010-1710 USA SN 0091-6749 J9 J ALLERGY CLIN IMMUN JI J. Allergy Clin. Immunol. PD JUL PY 2013 VL 132 IS 1 BP 118 EP U212 DI 10.1016/j.jaci.2013.01.022 PG 19 WC Allergy; Immunology SC Allergy; Immunology GA 173BF UT WOS:000321052300016 PM 23465659 ER PT J AU Uchimiya, M Orlov, A Ramakrishnan, G Sistani, K AF Uchimiya, Minori Orlov, Alexander Ramakrishnan, Girish Sistani, Karamat TI In situ and ex situ spectroscopic monitoring of biochar's surface functional groups SO JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS LA English DT Article DE Pyrolysis; Biomass; Thermochemical conversion; Volatile matter; Soil amendment ID CHARRED PLANT FRAGMENTS; VOLCANIC ASH SOILS; CARBONIZED CHARCOALS; NMR-SPECTROSCOPY; PYROLYSIS; MANURE; C-13; RECALCITRANCE; ACCUMULATION; RETENTION AB A number of studies concluded that peak pyrolysis temperature determines essential biochar properties: surface functional group, fixed carbon content, O/C, H/C, and BET surface area. Chemical (most importantly oxygen-containing surface functionalities), rather than physical (surface area) property of biochar dictates its ability to stabilize heavy metals in soil. Labile carbon fraction of biochar is rich in heavy metal coordinating functionalities, and recalcitrant carbon fraction is necessary for the long-term stability in soil. To produce desirable biochar properties With minimal time and cost, the slow pyrolysis platform must be optimized not only for the peak temperature, but additional parameters including the residence time and heating rate. In this study, time courses of biomass slow pyrolysis were investigated in the real time using a time-resolved (0.5 s) in situ Diffuse Reflectance Infrared Fourier Transform (DRIFTs) to continuously monitor the changes in surface functionality. The greatest change in surface functionality was observed at 200-500 degrees C from both in situ DRIFTs (during heating up at 10 degrees C min(-1) to reach the peak temperature) as well as ex situ (after a set residence time at the peak temperature) proximate, ultimate, attenuated total reflectance (ATR)-FTIR, and H-1 NMR analyses of slow/fast pyrolysis and activated biochars. The FTIR spectral features at respective temperatures during in situ pyrolysis of lignocellulosic feedstock matched the peak temperature of post-production analysis. The FTIR peaks attributable to the oxygen-containing functional groups dramatically diminished after 20 min residence time. Quantitative relationships were observed between parameters attributable to the aromatic characteristics: (1) baseline FTIR absorbance and (2) atomic H/C ratio with the fixed carbon content of biochar. Published by Elsevier B.V. C1 [Uchimiya, Minori] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Uchimiya, Minori; Orlov, Alexander; Ramakrishnan, Girish] SUNY Stony Brook, Dept Mat Sci & Engn, Stony Brook, NY 11794 USA. [Sistani, Karamat] ARS, USDA, Anim Waste Management Res Unit, Bowling Green, KY 42104 USA. RP Uchimiya, M (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 29 TC 31 Z9 31 U1 9 U2 154 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2370 J9 J ANAL APPL PYROL JI J. Anal. Appl. Pyrolysis PD JUL PY 2013 VL 102 BP 53 EP 59 DI 10.1016/j.jaap.2013.03.014 PG 7 WC Chemistry, Analytical; Spectroscopy SC Chemistry; Spectroscopy GA 174LZ UT WOS:000321158300007 ER PT J AU Yang, YW Adolph, AL Puyau, MR Vohra, FA Butte, NF Zakeri, IF AF Yang, Yunwen Adolph, Anne L. Puyau, Maurice R. Vohra, Firoz A. Butte, Nancy F. Zakeri, Issa F. TI Modeling energy expenditure in children and adolescents using quantile regression SO JOURNAL OF APPLIED PHYSIOLOGY LA English DT Article DE quantile regression; energy expenditure; obesity; childhood; heart rate; physical activity; accelerometry ID PHYSICAL-ACTIVITY; ESTIMATORS; AGE AB Advanced mathematical models have the potential to capture the complex metabolic and physiological processes that result in energy expenditure (EE). Study objective is to apply quantile regression (QR) to predict EE and determine quantile-dependent variation in covariate effects in nonobese and obese children. First, QR models will be developed to predict minute-by-minute awake EE at different quantile levels based on heart rate (HR) and physical activity (PA) accelerometry counts, and child characteristics of age, sex, weight, and height. Second, the QR models will be used to evaluate the covariate effects of weight, PA, and HR across the conditional EE distribution. QR and ordinary least squares (OLS) regressions are estimated in 109 children, aged 5-18 yr. QR modeling of EE outperformed OLS regression for both nonobese and obese populations. Average prediction errors for QR compared with OLS were not only smaller at the median tau = 0.5 (18.6 vs. 21.4%), but also substantially smaller at the tails of the distribution (10.2 vs. 39.2% at tau = 0.1 and 8.7 vs. 19.8% at tau = 0.9). Covariate effects of weight, PA, and HR on EE for the nonobese and obese children differed across quantiles (P < 0.05). The associations (linear and quadratic) between PA and HR with EE were stronger for the obese than nonobese population (P < 0.05). In conclusion, QR provided more accurate predictions of EE compared with conventional OLS regression, especially at the tails of the distribution, and revealed substantially different covariate effects of weight, PA, and HR on EE in nonobese and obese children. C1 [Yang, Yunwen; Zakeri, Issa F.] Drexel Univ, Dept Epidemiol & Biostat, Philadelphia, PA 19104 USA. [Adolph, Anne L.; Puyau, Maurice R.; Vohra, Firoz A.; Butte, Nancy F.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX USA. RP Butte, NF (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM nbutte@bcm.edu FU United States Department of Agriculture/Agricultural Research Service [58-6250-0-008]; National Institute of Diabetes and Digestive and Kidney Diseases Grant [R01 DK074387] FX This project has been funded with federal funds from the United States Department of Agriculture/Agricultural Research Service under Cooperative Agreement no. 58-6250-0-008, and National Institute of Diabetes and Digestive and Kidney Diseases Grant R01 DK074387. NR 22 TC 1 Z9 1 U1 1 U2 12 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 8750-7587 J9 J APPL PHYSIOL JI J. Appl. Physiol. PD JUL PY 2013 VL 115 IS 2 BP 251 EP 259 DI 10.1152/japplphysiol.00295.2013 PG 9 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA 183MH UT WOS:000321819000014 PM 23640591 ER PT J AU Benson, LV Ramsey, DK Stahle, DW Petersen, KL AF Benson, L. V. Ramsey, D. K. Stahle, D. W. Petersen, K. L. TI Some thoughts on the factors that controlled prehistoric maize production in the American Southwest with application to southwestern Colorado SO JOURNAL OF ARCHAEOLOGICAL SCIENCE LA English DT Article DE Maize agriculture; Southwestern Colorado ID MICROBIAL ACTIVITY; ORGANIC-CARBON; US SOUTHWEST; TILLAGE; MINERALIZATION; TEMPERATURE; CLIMATE; MONSOON; REGION AB In this paper, we present a model of prehistoric southwestern Colorado maize productivity. The model is based on a tree-ring reconstruction of water-year precipitation for Mesa Verde for the period A.D. 480 to 2011. Correlation of historic Mesa Verde precipitation with historic precipitation at 11 other weather stations enabled the construction of an elevation-dependent precipitation function. Prehistoric water-year precipitation values for Mesa Verde together with the elevation-dependent precipitation function allowed construction of the elevation of southwest Colorado precipitation contours for each year since A.D. 480, including the 30-cm contour, which represents the minimum amount of precipitation necessary for the production of maize and the 50-cm contour, which represents the optimum amount of precipitation necessary for the production of maize. In this paper, calculations of prehistoric maize productivity and field life for any specific elevation are also demonstrated. These calculations were performed using organic nitrogen measurements made on seven southwestern Colorado soil groups together with values of reconstructed water-year precipitation and estimations of the organic nitrogen mineralization rate. Published by Elsevier Ltd. C1 [Benson, L. V.] Univ Colorado, Museum Nat Hist, Boulder, CO 80302 USA. [Ramsey, D. K.] Nat Resources Conservat Serv, USDA, Hesperus, CO USA. [Stahle, D. W.] Univ Arkansas, Dept Geosci, Fayetteville, AR 72701 USA. [Petersen, K. L.] Univ Utah, Dept Geog, Salt Lake City, UT 84112 USA. RP Benson, LV (reprint author), Univ Colorado, Museum Nat Hist, 602 Pine St, Boulder, CO 80302 USA. EM lbenson@usgs.gov FU National Science Foundation [DEB-0816400]; NOAA [NA08OAR4310727] FX Much of this research was supported by the National Science Foundation under grant DEB-0816400 (Benson) and NOAA grant number NA08OAR4310727 (Stahle). This work supports the Village Ecodynamics Project (VEP) whose objectives include agent-based modeling of the interactions between prehistoric Native Americans who occupied the Four Corners region of the American Southwest, the landscape they occupied, and the climate that impacted the region during the late-thirteenth century. The authors thank Matthew Salzer for access to tree-ring-width data from Sap Francisco Peaks Bristlecone Pines and to Connie Woodhouse and Jeffrey Lukas for access to updated tree-ring-width data from the Almagre Mountain Bristlecone Pines. The Almagre Mountain C chronology was compiled by Craig Brunstein, using his new collection through 1994, plus D. Graybill's collection through 1983, and V. LaMarche's original chronology through 1968. Daniel Griffin was instrumental in measuring the Douglas fir ring widths and Dorian J. Burnette contributed to the statistical analyses. Stefani Crabtree created Supplementary Fig. 1. Jim Ashby, formerly with the Western Regional Climate Center, went beyond the call of duty to create the historical FFD data sets for the eight weather stations. Timothy Kohler reviewed earlier versions of this manuscript and made a number of excellent suggestions regarding its improvement. Eleanor Griffin was responsible for many of the soil elevation calculations. Thanks also to three anonymous reviewers and William Lipe for their suggestions on how the manuscript could be improved. NR 47 TC 4 Z9 4 U1 2 U2 17 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0305-4403 EI 1095-9238 J9 J ARCHAEOL SCI JI J. Archaeol. Sci. PD JUL PY 2013 VL 40 IS 7 BP 2869 EP 2880 DI 10.1016/j.jas.2013.03.013 PG 12 WC Anthropology; Archaeology; Geosciences, Multidisciplinary SC Anthropology; Archaeology; Geology GA 164NS UT WOS:000320417400001 ER PT J AU Homan, HJ LeJeune, JT Pearl, DL Seamans, TW Slowik, AA Morasch, MR Linz, GM AF Homan, H. J. LeJeune, J. T. Pearl, D. L. Seamans, T. W. Slowik, A. A. Morasch, Mark R. Linz, G. M. TI Use of dairies by postreproductive flocks of European starlings SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE behavior; dairy; radio telemetry; starling ID ESCHERICHIA-COLI; ASSOCIATION; SALMONELLA; FEEDLOTS; CATTLE; O157; OHIO AB Knowledge of the behavior and movement patterns of European starlings (Sturnus vulgaris L.) is important to wildlife managers that seek to resolve conflicts at livestock facilities. We captured and radio tagged 10 starlings at each of 5 dairies in northeastern Ohio. From September 19 to October 31, 2007, we obtained sufficient data from 40 birds to study their behavior and movements. The birds visited the dairies where they were initially captured (home sites) on 85% of the days, spending 58% of each day at the dairies. Onsite arrival and departure times were 2.5 h after sunrise and 3.1 h before sunset. Daily visits by radio-tagged cohorts from the other dairies were greatest for the 2 most proximate dairies (1.3 km apart), with number of visits between this pairing >7x that of the 9 other pairings combined (4.1-6.5 km apart). Two birds used their home sites intermittently as roosts, arriving 3.8 h before sunset and departing 0.2 h after sunrise. In addition to using home-site roosts, these birds also used a distant roost (22 km) that was used by 36 of the 40 birds. The efficacy of starling management programs, especially lethal management, depends on degree of site fidelity, use of other facilities, and roosting behavior. For example, starlings that use dairies as roosting sites may require a different management strategy than required at dairies used as daytime sites because of differences in arrival and departure behavior. Our research will help resource managers evaluate current management strategies already in place and change them, if needed, to fit the behavior profile of starlings using dairies and other types of livestock facilities. C1 [Homan, H. J.; Slowik, A. A.; Linz, G. M.] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. [LeJeune, J. T.; Morasch, Mark R.] Ohio State Univ, Food Anim Hlth Res Program, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Pearl, D. L.] Univ Guelph, Dept Populat Med, Ontario Vet Coll, Guelph, ON N1G 2W1, Canada. [Seamans, T. W.] Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. RP Homan, HJ (reprint author), Anim Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. EM jeffrey.h.honaan@aphis.usda.gov FU USDA Cooperative State Research, Education and Extension Service, Epidemiological Approaches to Food Safety Program of the National Research Initiative, Washington, DC [2006-35212-17039] FX We thank the following for their support and assistance with our project: R. Beason, J. Bourassa, N. Bower, C. Peery, C. Kauffman, J. Kauffman, R. Kauffman, and L. Penry of the National Wildlife Research Center; N. Bower and M. van den Tillart of Lotek Wireless Inc.; C. Peery, University of Idaho, Boise; R. Kauffman, Ohio Agricultural Research and Development Center, Wooster; and C. and J. Kauffman, volunteers, Shreve, OH. The research was supported, in part, by the USDA Cooperative State Research, Education and Extension Service, Epidemiological Approaches to Food Safety Program of the National Research Initiative (Grant number 2006-35212-17039), Washington, DC. Animal care and use procedures were followed in accordance with USDA Wildlife Services' National Wildlife Research Center IACUC guidelines (NWRC Study Protocol QA-1464, Study Director: H. J. Homan). Use of commercial products does not constitute an endorsement. NR 26 TC 5 Z9 5 U1 1 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 J9 J DAIRY SCI JI J. Dairy Sci. PD JUL PY 2013 VL 96 IS 7 BP 4487 EP 4493 DI 10.3168/jds.2013-6712 PG 7 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA 168YZ UT WOS:000320745200038 PM 23684017 ER PT J AU Yan, ZJ Liu, PP Li, YH Ma, L Alva, A Dou, ZX Chen, Q Zhang, FS AF Yan, Zhengjuan Liu, Pengpeng Li, Yuhong Ma, Lin Alva, Ashok Dou, Zhengxia Chen, Qing Zhang, Fusuo TI Phosphorus in China's Intensive Vegetable Production Systems: Overfertilization, Soil Enrichment, and Environmental Implications SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID CATTLE FEEDLOT MANURE; ORGANIC PHOSPHORUS; AMENDED SOILS; PLANT; NUTRIENT; RISK; AVAILABILITY; REQUIREMENT; MANAGEMENT; EFFICIENCY AB China's vegetable production has experienced a rapid growth in recent years. Total production amounted to 522.7 million Mg (1 Mg = 10(6) g) in 2009, which was more than nine times that in 1980 and represented >50% of the world production. Meanwhile, excessive use of animal manures and chemical fertilizers in vegetable fields has brought various production and environmental challenges, including excessive accumulation of nutrients in soils and accelerated water pollution problems. In this study, we have evaluated the current status of phosphorus (P) in China's intensive vegetable production systems based on data summarized from nearly 100 publications plus results from our recent experiments. Gross overfertilization occurred in greenhouse (571 kg P ha(-1)) and open-field (117 kg P ha(-1)) vegetable systems compared with P removal in harvested crops (44 and 25 kg P ha(-1)) per season. Excess P input led to soil enrichment of labile P, measured as Olsen-P, averaging 179 (greenhouses) and 100 mg P kg(-1) (open fields) in the 0- to 20-cm soil depth, and in some cases led to P leaching, as evidenced by increases in Olsen-P and CaCl2 - P at the 40- to 60-cm soil depth. The vast majority of vegetable soils had Olsen-P exceeding the critical level (46.0-58.0 mg P kg(-1)) for optimum vegetable yield. Innovative policies and strategies are urgently needed to implement science-based nutrient management practices to attain sustainable vegetable production while protecting natural and environmental resources. C1 [Yan, Zhengjuan; Liu, Pengpeng; Li, Yuhong; Ma, Lin; Chen, Qing; Zhang, Fusuo] China Agr Univ, Coll Resources & Environm Sci, Beijing 100193, Peoples R China. [Yan, Zhengjuan; Liu, Pengpeng; Li, Yuhong; Ma, Lin; Chen, Qing; Zhang, Fusuo] China Agr Univ, Ctr Resources Environm & Food Secur, Beijing 100193, Peoples R China. [Ma, Lin] Wageningen Univ, Dep Soil Qual, NL-6700 AA Wageningen, Netherlands. [Alva, Ashok] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Dou, Zhengxia] Univ Penn, Sch Vet Med, Ctr Anim Hlth & Prod, Kennett Sq, PA 19348 USA. RP Chen, Q (reprint author), China Agr Univ, Coll Resources & Environm Sci, 2 Yuanmingyuan Xilu, Beijing 100193, Peoples R China. EM qchen@cau.edu.cn RI Ma, Lin/F-3770-2012; OI Ma, Lin/0000-0002-2895-9067; ma, lin/0000-0003-1761-0158 FU Special Fund for Agriculture Profession of the Ministry of Agriculture [201103003]; planning subject of 'the 12th Five-Year-Plan' in the National Science and Technology for the Rural Development in China [2012BAD15B01-4]; program of Innovative Research Team of Beijing Fruit Vegetable Industry FX The authors thank Drs. Tom Sims and Oene Oenema for their kind and constructive comments and suggestions on this manuscript. The authors also appreciate the following sponsors of this study: Special Fund for Agriculture Profession (201103003) of the Ministry of Agriculture, the planning subject of 'the 12th Five-Year-Plan' in the National Science and Technology for the Rural Development in China (2012BAD15B01-4), and the program of Innovative Research Team of Beijing Fruit Vegetable Industry. NR 83 TC 21 Z9 28 U1 11 U2 85 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2013 VL 42 IS 4 BP 982 EP 989 DI 10.2134/jeq2012.0463 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 183NH UT WOS:000321822300005 PM 24216350 ER PT J AU Rogers, CW Brye, KR Norman, RJ Gbur, EE Mattice, JD Parkin, TB Roberts, TL AF Rogers, Christopher W. Brye, Kristofor R. Norman, Richard J. Gbur, Edward E. Mattice, John D. Parkin, Timothy B. Roberts, Trenton L. TI Methane Emissions from Drill-Seeded, Delayed-Flood Rice Production on a Silt-Loam Soil in Arkansas SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID NITROUS-OXIDE EMISSIONS; WATER MANAGEMENT; CALIFORNIA RICE; LOUISIANA RICE; PADDY SOIL; GAS FLUXES; FIELDS; TRANSPORT; PLANTS; CHAMBER AB Rice (Oryza sativa L.) production is unique among staple food crops because the majority of the growing season typically occurs under flooded-soil conditions. Flooding the soil leads to anaerobic conditions, which are a precursor to methane (CH4) production. However, no known research has investigated CH4 emissions from the drill-seeded, delayed-flood rice production system common in Arkansas, the leading rice-producing state in the United States. Therefore, research was conducted in 2011 to determine the effects of vegetation (rice and bare soil), chamber location (in-and between-rice rows), and nitrogen (N) fertilization (optimal and no N) on CH4 emissions from a silt-loam soil. Methane fluxes measured weekly from flooding until flood release were affected by vegetation, chamber location, and sample date (P < 0.05). In-row CH4 fluxes were < 0.7 mg CH4-C m(-2) h(-1) until 20 d after flooding (DAF) and < 1.0 mg CH4-C m(-2) h(-1) from between-row and bare soil until 41 DAF and were unaffected by fertilization over time. The largest weekly measured CH4 flux (31.9 mg CH4-C m(-2) h(-1)) was observed from in-row rice at 41 DAF. Post-flood-release CH4 fluxes were affected by vegetation, fertilization, chamber placement, and sample date (P < 0.05) and accounted for approximately 3 to 7% of the season-long CH4 emissions. Methane emissions averaged 195 kg CH4-C ha(-1) per growing season and were unaffected by fertilization. Direct measurement of CH4 emissions from drillseeded, delayed-flood rice grown on a silt-loam soil will improve the accuracy of assessments of the carbon footprint and long-term sustainability of rice. C1 [Rogers, Christopher W.; Brye, Kristofor R.; Norman, Richard J.; Mattice, John D.; Roberts, Trenton L.] Univ Arkansas, Dep Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Gbur, Edward E.] Univ Arkansas, Agr Stat Lab, Fayetteville, AR 72701 USA. [Parkin, Timothy B.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. RP Rogers, CW (reprint author), Univ Arkansas, Dep Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. EM cwrogers@uark.edu FU Arkansas Rice Research and Promotion Board FX The authors thank Travis Gasnier, Donna Frizzell, and Anthony Fulford for providing valuable field and laboratory assistance. This work was supported by The Arkansas Rice Research and Promotion Board. NR 62 TC 6 Z9 7 U1 2 U2 23 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2013 VL 42 IS 4 BP 1059 EP 1069 DI 10.2134/jeq2012.0502 PG 11 WC Environmental Sciences SC Environmental Sciences & Ecology GA 183NH UT WOS:000321822300012 PM 24216357 ER PT J AU Bolster, CH Vadas, PA AF Bolster, Carl H. Vadas, Peter A. TI Sensitivity and Uncertainty Analysis for the Annual Phosphorus Loss Estimator Model SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID WATER-QUALITY MODELS; GOODNESS-OF-FIT; RISK-ASSESSMENT; RUNOFF; QUANTIFICATION; VALIDATION; INDEXES; MANURE AB Models are often used to predict phosphorus (P) loss from agricultural fields. Although it is commonly recognized that model predictions are inherently uncertain, few studies have addressed prediction uncertainties using P loss models. In this study we assessed the effect of model input error on predictions of annual P loss by the Annual P Loss Estimator (APLE) model. Our objectives were (i) to conduct a sensitivity analyses for all APLE input variables to determine which variables the model is most sensitive to, (ii) to determine whether the relatively easy-to-implement first-order approximation (FOA) method provides accurate estimates of model prediction uncertainties by comparing results with the more accurate Monte Carlo simulation (MCS) method, and (iii) to evaluate the performance of the APLE model against measured P loss data when uncertainties in model predictions and measured data are included. Our results showed that for low to moderate uncertainties in APLE input variables, the FOA method yields reasonable estimates of model prediction uncertainties, although for cases where manure solid content is between 14 and 17%, the FOA method may not be as accurate as the MCS method due to a discontinuity in the manure P loss component of APLE at a manure solid content of 15%. The estimated uncertainties in APLE predictions based on assumed errors in the input variables ranged from +/- 2 to 64% of the predicted value. Results from this study highlight the importance of including reasonable estimates of model uncertainty when using models to predict P loss. C1 [Bolster, Carl H.] USDA ARS, Bowling Green, KY 42104 USA. [Vadas, Peter A.] USDA ARS, Madison, WI 53706 USA. RP Bolster, CH (reprint author), USDA ARS, 230 Bennett Ln, Bowling Green, KY 42104 USA. EM carl.bolster@ars.usda.gov FU USDA-ARS National Program:Manure and Byproduct Utilization [214] FX The authors thank R.D. Harmel for providing a spreadsheet to check calculations of the degree of overlap correction factor and the reviewers of the manuscript for helpful suggestions. This research was part of USDA-ARS National Program 214: Manure and Byproduct Utilization. The authors declare no competing financial interest. Mention of a trade name, proprietary product, or vendor is for information only and does not guarantee or warrant the product by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 45 TC 8 Z9 8 U1 1 U2 20 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 EI 1537-2537 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2013 VL 42 IS 4 BP 1109 EP 1118 DI 10.2134/jeq2012.0418 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA 183NH UT WOS:000321822300017 PM 24216362 ER PT J AU Bartelt-Hunt, SL DeVivo, S Johnson, L Snow, DD Kranz, WL Mader, TL Shapiro, CA van Donk, SJ Shelton, DP Tarkalson, DD Zhang, TC AF Bartelt-Hunt, Shannon L. DeVivo, Shannon Johnson, Leslie Snow, Daniel D. Kranz, William L. Mader, Terry L. Shapiro, Charles A. van Donk, Simon J. Shelton, David P. Tarkalson, David D. Zhang, Tian C. TI Effect of Composting on the Fate of Steroids in Beef Cattle Manure SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article ID ANIMAL FEEDING OPERATIONS; POULTRY LITTER; LAGOON SAMPLES; ESTROGENS; RUNOFF; 17-BETA-ESTRADIOL; WATER; PROGESTERONE; HORMONES; WASTE AB In this study, the fate of steroid hormones in beef cattle manure composting is evaluated. The fate of 16 steroids and metabolites was evaluated in composted manure from beef cattle administered growth promotants and from beef cattle with no steroid hormone implants. The fate of estrogens (primary detected as estrone), androgens, progesterone, and the fusarium metabolite and implant a-zearalanol was monitored in manure compost piles. First-order decay rates were calculated for steroid half-lives in compost and ranged from 8 d for androsterone to 69 d for 4-androstenedione. Other steroid concentration data could not be fit to first-order decay models, which may indicate that microbial processes may result in steroid production or synthesis in composting systems. We demonstrate that composting is an effective strategy to remove steroid hormones from manure. Total steroid hormone removal in composted beef cattle manure ranged from 79 to 87%. C1 [Bartelt-Hunt, Shannon L.; DeVivo, Shannon; Zhang, Tian C.] Univ Nebraska Lincoln, Dep Civil Engn, Peter Kiewit Inst, Omaha, NE 68182 USA. [Snow, Daniel D.] Univ Nebraska, Sch Nat Resources, Lincoln, NE 68583 USA. [Johnson, Leslie; Kranz, William L.; Shapiro, Charles A.; Shelton, David P.] Univ Nebraska Lincoln, Northeast Res & Extens Ctr, Concord, NE 68728 USA. [Mader, Terry L.] Mader Consulting LLC, Gretna, NE 68028 USA. [van Donk, Simon J.] Univ Nebraska Lincoln, West Cent Res & Extens Ctr, North Platte, NE 69101 USA. [Tarkalson, David D.] USDA ARS, Northwest Irrigat & Soils Res Lab, Kimberly, ID USA. RP Bartelt-Hunt, SL (reprint author), Univ Nebraska Lincoln, Dep Civil Engn, Peter Kiewit Inst, Omaha, NE 68182 USA. EM sbartelt2@unl.edu FU USEPA Science [R833423] FX The study was funded in part by a USEPA Science to Achieve Results (STAR) award no. R833423. NR 29 TC 5 Z9 5 U1 2 U2 27 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2013 VL 42 IS 4 BP 1159 EP 1166 DI 10.2134/jeq2013.01.0024 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 183NH UT WOS:000321822300022 PM 24216367 ER PT J AU Del Grosso, SJ White, JW Wilson, G Vandenberg, B Karlen, DL Follett, RF Johnson, JMF Franzluebbers, AJ Archer, DW Gollany, HT Liebig, MA Ascough, J Reyes-Fox, M Pellack, L Starr, J Barbour, N Polumsky, RW Gutwein, M James, D AF Del Grosso, S. J. White, J. W. Wilson, G. Vandenberg, B. Karlen, D. L. Follett, R. F. Johnson, J. M. F. Franzluebbers, A. J. Archer, D. W. Gollany, H. T. Liebig, M. A. Ascough, J. Reyes-Fox, M. Pellack, L. Starr, J. Barbour, N. Polumsky, R. W. Gutwein, M. James, D. TI Introducing the GRACEnet/REAP Data Contribution, Discovery, and Retrieval System SO JOURNAL OF ENVIRONMENTAL QUALITY LA English DT Article AB Difficulties in accessing high-quality data on trace gas fluxes and performance of bioenergy/bioproduct feedstocks limit the ability of researchers and others to address environmental impacts of agriculture and the potential to produce feedstocks. To address those needs, the GRACEnet (Greenhouse gas Reduction through Agricultural Carbon Enhancement network) and REAP (Renewable Energy Assessment Project) research programs were initiated by the USDA Agricultural Research Service (ARS). A major product of these programs is the creation of a database with greenhouse gas fluxes, soil carbon stocks, biomass yield, nutrient, and energy characteristics, and input data for modeling cropped and grazed systems. The data include site descriptors (e.g., weather, soil class, spatial attributes), experimental design (e.g., factors manipulated, measurements performed, plot layouts), management information (e.g., planting and harvesting schedules, fertilizer types and amounts, biomass harvested, grazing intensity), and measurements (e.g., soil C and N stocks, plant biomass amount and chemical composition). To promote standardization of data and ensure that experiments were fully described, sampling protocols and a spreadsheet-based data-entry template were developed. Data were first uploaded to a temporary database for checking and then were uploaded to the central database. A Web-accessible application allows for registered users to query and download data including measurement protocols. Separate portals have been provided for each project (GRACEnet and REAP) at nrrc.ars.usda.gov/slgracenet/#/Home and nrrc.ars.usda.gov/slreap/#/Home. The database architecture and data entry template have proven flexible and robust for describing a wide range of field experiments and thus appear suitable for other natural resource research projects. C1 [Del Grosso, S. J.; Follett, R. F.; James, D.] USDA ARS, Soil Plant Nutrient Res, Ft Collins, CO 80526 USA. [White, J. W.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85239 USA. [Wilson, G.] Iowa State Univ, Inst Design Res & Outreach, Ames, IA USA. [Vandenberg, B.; Gutwein, M.] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. [Karlen, D. L.; Pellack, L.; James, D.] USDA ARS, Soil Water & Air Resources Res Unit, Ames, IA 50011 USA. [Johnson, J. M. F.; Starr, J.; Barbour, N.] USDA ARS, Soil Management Res, Morris, MN 56267 USA. [Franzluebbers, A. J.] USDA ARS, Raleigh, NC 27695 USA. [Archer, D. W.; Liebig, M. A.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Polumsky, R. W.] USDA ARS, Columbia Plateau Conservat Res Ctr, Adams, OR 97810 USA. RP Del Grosso, SJ (reprint author), USDA ARS, Soil Plant Nutrient Res, 2150 Ctr Ave,Bldg D,Ste 100, Ft Collins, CO 80526 USA. EM steve.delgrosso@ars.usda.gov OI Archer, David/0000-0002-4816-7040; Johnson, Jane/0000-0002-1687-4007 FU USDA-ARS, USDA- ARS GRACEnet project; USDA-ARS, USDA- ARS REAP project; North Central Regional SunGrant Center at South Dakota State University; USDOE Office of Biomass Programs [DE-FC36-05GO85041] FX The authors thank the GRACEnet and REAP data contributors for their individual research efforts, USDA-ARS National Program Staff, and members of the data management and curation teams. Funding for this project is provided by the USDA-ARS, as part of the USDA- ARS GRACEnet and USDA- ARS REAP projects. Additional funding was provided from the North Central Regional SunGrant Center at South Dakota State University through a grant provided by the USDOE Office of Biomass Programs under award number DE-FC36-05GO85041. NR 18 TC 14 Z9 14 U1 0 U2 24 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0047-2425 J9 J ENVIRON QUAL JI J. Environ. Qual. PD JUL-AUG PY 2013 VL 42 IS 4 BP 1274 EP 1280 DI 10.2134/jeq2013.03.0097 PG 7 WC Environmental Sciences SC Environmental Sciences & Ecology GA 183NH UT WOS:000321822300034 PM 24216379 ER PT J AU Park, J Stanley, D Kim, Y AF Park, Jiyeong Stanley, David Kim, Yonggyun TI Rac1 mediates cytokine-stimulated hemocyte spreading via prostaglandin biosynthesis in the beet armyworm, Spodoptera exigua SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Rac1; Plasmatocyte-spreading peptide; 5-Hydroxytryptamine; Eicosanoid; Octopamine; Spodoptera exigua ID DROSOPHILA-MELANOGASTER; CELL-ADHESION; RHO-GTPASES; PROTEIN; EICOSANOIDS; INTEGRIN; INSECTS; VIRULENCE; BEHAVIOR; IMMUNITY AB Cell spreading is an integral component of insect hemocytic immune reactions to infections and invasions. Cell spreading is accomplished by cytoskeleton rearrangement, which is activated by three major immune mediators, biogenic monoamines, plasmatocyte-spreading peptide (PSP), and eicosanoids, particularly prostaglandin E-2 (PGE(2)). However, little is known about how these immune mediators activate hemocyte spreading at the intra-cellular level. A small G protein, Rac1, acts in cytoskeleton arrangements in mammalian cells. Based on this information, we identified a Rac1 transcript (SeRac1) in hemocytes prepared from Spodoptera exigua. SeRac1 was expressed in most developmental stages and in the two main immunity-conferring tissues, hemocytes and fat body, in larvae. In response to bacterial challenge, its expression was up-regulated by >37-fold at 2 h post-injection and returned to a basal level about 2 h later. Silencing SeRac1 expression inhibited hemocyte spreading in response to three immune mediators, octopamine, 5-hydroxytryptamine, and PSP. Addition of PGE(2) to SeRac1-silenced larvae rescued the influence of these three mediators on hemocyte spreading. These compounds also increased phospholipase A(2) activity via SeRac1, which leads to prostaglandin biosynthesis. We infer that SeRac1 transduces OA, 5-HT, and PSP signaling via activating biosynthesis of prostaglandins and possibly other eicosanoids. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Park, Jiyeong; Kim, Yonggyun] Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. [Stanley, David] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65203 USA. RP Kim, Y (reprint author), Andong Natl Univ, Dept Bioresource Sci, Andong 760749, South Korea. EM hosanna@andong.ac.kr FU National Research Foundation, Korea [2010-0021050] FX This study was supported by a Basic Research Program grant (2010-0021050) of National Research Foundation, Korea to YK. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. All programs and services of the U.S. Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status, or handicap. NR 41 TC 4 Z9 4 U1 0 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD JUL PY 2013 VL 59 IS 7 BP 682 EP 689 DI 10.1016/j.jinsphys.2013.04.012 PG 8 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA 174SR UT WOS:000321176200005 PM 23660478 ER PT J AU Sun, LH Li, JG Zhao, H Shi, J Huang, JQ Wang, KN Xia, XJ Li, L Lei, XG AF Sun, Lv-Hui Li, Jun-Gang Zhao, Hua Shi, Jing Huang, Jia-Qiang Wang, Kang-Ning Xia, Xin-Jie Li, Li Lei, Xin Gen TI Porcine Serum Can Be Biofortified with Selenium to Inhibit Proliferation of Three Types of Human Cancer Cells SO JOURNAL OF NUTRITION LA English DT Article ID SELENOPROTEIN GENE-EXPRESSION; ENRICHED MILK-PROTEINS; PROSTATE-CANCER; METHYLSELENINIC ACID; COLON-CANCER; YOUNG-PIGS; APOPTOSIS; GROWTH; DEFICIENCY; PREVENTION AB Our objectives were to determine if porcine serum could be enriched with selenium (Se) by feeding pigs with high concentrations of dietary Se and if the Se-biofortified serum inhibited proliferation of 3 types of human cancer cells. In Expt. 1, growing pigs (8 wk old, n = 3) were fed 0.02 or 3.0 mg Se/kg (as sodium selenite) for 16 wk and produced serum with 0.5 and 5.4 mu mol/L Se, respectively. In Expt. 2, growing pigs (5 wk old, n = 6) were fed 0.3 or 1.0 mg Se/kg (as Se-enriched yeast) for 6 wk and produced serum with 2.6 and 6.2 mu mol/L Se, respectively. After the Se-biofortified porcine sera were added at 16% in RPMI 1640 to treat NCI-H446, DU145, and HTC116 cells for 144 h, they decreased (P < 0.05) the viability of the 3 types of human cancer cells by promoting apoptosis, compared with their controls. This effect was replicated only by adding the appropriate amount of methylseleninic acid to the control serum and was mediated by a downregulation of 8 cell cycle arrest genes and an upregulation of 7 apoptotic genes. Along with 6 previously reported selenoprotein genes, selenoprotein T (Selt), selenoprotein M (Selm), selenoprotein H (Selh), selenoprotein K (Selk), and selenoprotein N (Sepn1) were revealed to be strongly associated with the cell death-related signaling induced by the Se-enriched porcine serum. In conclusion, porcine serum could be biofortified with Se to effectively inhibit the proliferation of 3 types of human cancer cells and the action synchronized with a matrix of coordinated functional expression of multiple selenoprotein genes. C1 [Sun, Lv-Hui; Li, Jun-Gang; Zhao, Hua; Shi, Jing; Huang, Jia-Qiang; Wang, Kang-Ning; Xia, Xin-Jie; Lei, Xin Gen] Sichuan Agr Univ, Int Res Ctr Future Agr Human Hlth, Chengdu, Peoples R China. [Sun, Lv-Hui; Lei, Xin Gen] Cornell Univ, Dept Anim Sci, Ithaca, NY 14853 USA. [Li, Li] ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY USA. RP Lei, XG (reprint author), Sichuan Agr Univ, Int Res Ctr Future Agr Human Hlth, Chengdu, Peoples R China. EM XL20@cornell.edu FU Chinese Natural Science Foundation [30628019, 30871844, 30700585]; Chang Jiang Scholars Program from the Ministry of Education of People's Republic of China; NIH [DK 53018] FX Supported in part by the Chinese Natural Science Foundation projects 30628019, 30871844, and 30700585, the Chang Jiang Scholars Program from the Ministry of Education of People's Republic of China, and NIH DK 53018. NR 53 TC 11 Z9 11 U1 3 U2 21 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 J9 J NUTR JI J. Nutr. PD JUL PY 2013 VL 143 IS 7 BP 1115 EP 1122 DI 10.3945/jn.113.177410 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 170ZT UT WOS:000320894800014 PM 23677865 ER PT J AU Zheljazkov, VA Vick, B Ebelhar, W Buehring, N Astatkie, T AF Zheljazkov, Valtcho A. Vick, Brady Ebelhar, Wayne Buehring, Normie Astatkie, Tess TI Effect of N on Yield and Chemical Profile of Winter Canola in Mississippi SO JOURNAL OF OLEO SCIENCE LA English DT Article DE canola; fatty acids; nitrogen; Southeastern United States ID DIVERSE ENVIRONMENTS; PLANTING DATE; NITROGEN RATE; OIL CONTENT; SUNFLOWER; FERTILIZATION; EFFICIENCY; MUSTARD; HYBRID AB There is increased interest in winter canola as an oilseeds crop for production of oil or biodiesel in the southeastern United States, but research has been limited. The objective of this study was to evaluate the effect of N (0, 60, 120, 180 kg N ha(-1)) on productivity, oil content and oil composition of winter canola grown for two cropping seasons at three locations in Mississippi (Stoneville, and two locations at Verona: Verona upland silt loam, Verona-SL and Verona upland clay, Verona-C). Overall, increasing N application rates resulted in corresponding stepwise increase in seed yields in the two locations at Verona, whereas yields in the 60 and 120 kg N ha(-1) at Stoneville were not different from each other. Seed yields reached 3,383 and 3,166 kg ha(-4) in the 180 kg N treatment at Verona and at Stoneville, respectively. Oil yields were also increased with increasing N rates, however, oil yields at 60 and 120 kg N ha(-4) at Verona-C were not different from each other. Oil yields in the 180 kg N ha(-1) treatment reached 1,363 and 1,151 kg ha(-1) at Verona-SL and Stoneville, respectively. At Verona-SL location, higher N rates resulted in increased stearic acid compared to the lower N rates. However, the reverse effect was observed on the concentration of linolenic acid, which was lower at higher N rates. Also at that location, N application reduced the concentration of linoleic acid. At the Verona-C location, N application at 180 kg N ha(-1) reduced concentration of linolenic acid relative to the other fertility treatments. Overall, the increase in N application rates resulted in greater yield (kg FA ha(-1)) of palmitic, palmitoleic, stearic, oleic, linoleic, linolenic, arachidic, eicosanoic, behenic, lignoceric and nervonic acids in all three locations, with N at 0 kg ha(-1) providing the lowest yields and N at 180 kg ha(-1) providing the highest yields. Winter canola production in the hot humid environment of southeastern United States can be successful and could provide seed and oil yields comparable to yields from major winter canola production areas. C1 [Zheljazkov, Valtcho A.] Univ Wyoming, Sheridan Res & Extens Ctr, Sheridan, WY 82801 USA. [Vick, Brady] ARS, Sunflower Res Unit, USDA, Fargo, ND 58102 USA. [Ebelhar, Wayne] Mississippi State Univ, Delta Res & Extens Ctr, Stoneville, MS 38776 USA. [Buehring, Normie] Mississippi State Univ, North Mississippi Res & Extens Ctr, Verona, MS 38879 USA. [Astatkie, Tess] Dalhousie Univ, Fac Agr, Truro, NS B2N 5E3, Canada. RP Zheljazkov, VA (reprint author), Univ Wyoming, Sheridan Res & Extens Ctr, 663 Wyarno Rd, Sheridan, WY 82801 USA. EM vjeliazk@uwyo.edu RI Astatkie, Tess/J-7231-2012 OI Astatkie, Tess/0000-0002-9779-8789 FU Department of Energy for the project Feedstock for Sustainable Energy Systems in Mississippi FX Authors acknowledge the financial support by the Department of Energy for the project Feedstock for Sustainable Energy Systems in Mississippi. Authors thank Mr. Thomas Horgan, Mr. Leonard Cook and Mr. Davis Clark for their help in the field and laboratory. NR 23 TC 1 Z9 1 U1 3 U2 25 PU JAPAN OIL CHEMISTS SOC PI TOKYO PA YUSHI KOGYO KAIKAN BLDG, 13-11, NIHONBASHI 3-CHOME, CHUO-KU, TOKYO, 103-0027, JAPAN SN 1345-8957 EI 1347-3352 J9 J OLEO SCI JI J. Oleo Sci. PD JUL PY 2013 VL 62 IS 7 BP 453 EP 458 DI 10.5650/jos.62.453 PG 6 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 175LP UT WOS:000321232500002 PM 23823910 ER PT J AU Brown, P Gipson, C AF Brown, Patricia Gipson, Chester TI A word from OLAW and USDA SO LAB ANIMAL LA English DT Editorial Material C1 [Brown, Patricia] NIH, OLAW, OER, OD,HHS, Bethesda, MD 20892 USA. [Gipson, Chester] USDA, APHIS, AC, Tucson, AZ USA. RP Brown, P (reprint author), NIH, OLAW, OER, OD,HHS, Bethesda, MD 20892 USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 0093-7355 J9 LAB ANIMAL JI Lab Anim. PD JUL PY 2013 VL 42 IS 7 BP 244 EP 244 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA 174MJ UT WOS:000321159400016 PM 23783311 ER PT J AU Shepherd, BS Rees, CB Sepulveda-Villet, OJ Palmquist, DE Binkowski, FP AF Shepherd, Brian S. Rees, Christopher B. Sepulveda-Villet, Osvaldo J. Palmquist, Debra E. Binkowski, Fred P. TI Identification of Gender in Yellow Perch by External Morphology: Validation in Four Geographic Strains and Effects of Estradiol SO NORTH AMERICAN JOURNAL OF AQUACULTURE LA English DT Article ID SEXUAL-DIFFERENTIATION; 3-SPINED STICKLEBACK; PIMEPHALES-PROMELAS; FISH REPRODUCTION; CHINOOK SALMON; FLAVESCENS; GROWTH; CYCLE; SIZE; PCR AB External morphological criteria that enable the rapid determination of gender have been developed for Yellow Perch Perca flavescens. Criteria are based upon (1) shape of the urogenital papilla (UGP), (2) size of the UGP relative to the anal (AN) opening, and (3) coloration of the UGP. In females, the UGP appeared (1) rounded at the anterior margin, (2) pointed at the posterior margin and had a V or U shape (crescent shaped in mature animals), (3) generally lacked reddish coloration, and (4) narrower relative to the AN. In males, the UGP (1) appeared circular or oval around the entire margin, (2) typically displayed reddish coloration, and (3) was generally wider relative to the AN. To verify accuracy of these criteria, gender was verified internally in perch of various sizes, sex, and maturity from four domesticated geographic strains (n = 1,389). For all perch tested, accuracy was 97.3% for both sexes, 98.8% for females, and 95.9% for males. To experimentally verify accuracy of these criteria, juvenile Yellow Perch (n = 913) were treated with dietary 17-estradiol (E2; 15mg/kg diet) or a control diet. Accuracy was 97.7% for control females and 95.1% for control males, which diminished to 63.9% for the E2-treated females and 57.6% for E2-treated males. We developed a gender identification algorithm that will enable sorting of sexes for many uses including (1) collection of broodstock by new aquaculture producers, (2) management of broodstocks for existing producers, (3) improved selection criteria for genetic selection programs, (4) studies on gender-specific differences in Yellow Perch physiology, and (5) the unharmed release of fish in field and aquaculture settings. Uncoupling of external UGP morphology from actual gender in E2-treated perch can enable producers and biologists to detect exposure to estrogenic compounds in areas where endocrine disruption is suspected. Received October 11, 2012; accepted March 5, 2013 C1 [Shepherd, Brian S.; Rees, Christopher B.; Sepulveda-Villet, Osvaldo J.] Univ Wisconsin, USDA, ARS, Sch Freshwater Sci, Milwaukee, WI 53204 USA. [Palmquist, Debra E.] USDA ARS, Midwest Area Stat Unit, Peoria, IL 61604 USA. [Binkowski, Fred P.] Univ Wisconsin, Sch Freshwater Sci, Milwaukee, WI 53204 USA. RP Shepherd, BS (reprint author), Univ Wisconsin, USDA, ARS, Sch Freshwater Sci, 600 East Greenfield Ave, Milwaukee, WI 53204 USA. EM brian.shepherd@ars.usda.gov FU U.S. Department of Agriculture (USDA), Agricultural Research Service CRIS project [3655-31320-002-00D] FX The authors gratefully acknowledge the assistance of Christopher Groff, Jeff Neuse, Jill Paddock, Michele Shepherd, and Steven Yeo (School of Freshwater Sciences, University of Wisconsin-Milwaukee), and critical comments by anonymous reviewers. This study was funded by U.S. Department of Agriculture (USDA), Agricultural Research Service CRIS project 3655-31320-002-00D. The views contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Government. Mention of trade names, proprietary products, or specific equipment does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products that may be suitable. This manuscript is submitted for publication with the understanding that the U.S. Government is authorized to reproduce and distribute reprints for governmental purposes. NR 39 TC 0 Z9 0 U1 1 U2 12 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1522-2055 J9 N AM J AQUACULT JI N. Am. J. Aqualcult. PD JUL 1 PY 2013 VL 75 IS 3 BP 361 EP 372 DI 10.1080/15222055.2013.783520 PG 12 WC Fisheries SC Fisheries GA 180PV UT WOS:000321608000005 ER PT J AU Moreno, JP Johnston, CA El-Mubasher, AA Papaioannou, MA Tyler, C Gee, M Foreyt, JP AF Moreno, Jennette Palcic Johnston, Craig A. El-Mubasher, Abeer A. Papaioannou, Maria A. Tyler, Chermaine Gee, Molly Foreyt, John P. TI Peanut consumption in adolescents is associated with improved weight status SO NUTRITION RESEARCH LA English DT Article DE Peanuts; Adolescents; Overweight; Diet quality; Mexican American ID CARDIOVASCULAR-DISEASE; MAGNESIUM-DEFICIENCY; NUT CONSUMPTION; FOOD-INTAKE; LOW-FAT; OBESITY; RISK; CHILDREN; ADULTS; DIETS AB Studies have shown an association between nut consumption and health benefits in adults such as lower lipid levels, lowerbody mass indices, and reduced risk of coronary artery disease. Few studies have demonstrated these health benefits in children. To determine the association between peanut consumption and weight, intake of nutrients of concern, high-density lipoprotein, low-density lipoprotein, and cholesterol in Mexican American children, baseline data from 262 sixth-grade students (48% female) in a school-based weight management program were analyzed to compare differences between peanut and non-peanut eaters. It was hypothesized that Mexican American children who consume peanuts will be less overweight and have a better nutrient and lipid profile when compared to those who do not eat peanuts. Participants completed a food frequency questionnaire as a baseline dietary assessment before beginning the program. Children were identified as either a peanut consumer (n = 100) or non-peanut consumer (n = 162). Body mass index measurements were taken on all participants. A smaller sample of participants submitted blood for lipid analysis. Analyses revealed that children in the peanut consumer group were less likely to be overweight or obese than children in the non-peanut consumer group (x(2)= 13.9, P =.001), had significantly higher intakes of several vitamins and micronutrients (i.e., magnesium, vitamin E), and had lower low-density lipoprotein and total cholesterol levels. These results illustrate that consumption of peanuts and/or peanut butter is associated with lower weight status, improved diet, and lipid levels among Mexican American children. Future research is needed to clarify the role of peanut consumption in children's overall health. (C) 2013 Elsevier Inc. All rights reserved. C1 [Moreno, Jennette Palcic; Johnston, Craig A.; El-Mubasher, Abeer A.; Papaioannou, Maria A.; Tyler, Chermaine; Foreyt, John P.] USDA ARS Childrens Nutr Res Ctr, Baylor Coll Med, Dept Pediat Nutr, Houston, TX 77030 USA. [Johnston, Craig A.; Gee, Molly; Foreyt, John P.] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. RP Moreno, JP (reprint author), Baylor Coll Med, Dept Pediat Nutr, Houston, TX 77030 USA. EM palcic@bcm.edu FU United States Department of Agriculture [ARS 2533759358] FX We are extremely grateful to the staff of YES Preparatory Academy for their dedication to improving the lives of children. Research conducted for this study was supported by a grant from the United States Department of Agriculture (ARS 2533759358). There are no conflicts of interest to disclose. NR 32 TC 4 Z9 5 U1 4 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUL PY 2013 VL 33 IS 7 BP 552 EP 556 DI 10.1016/j.nutres.2013.05.005 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 184IC UT WOS:000321882300005 PM 23827129 ER PT J AU Young, LR Kurzer, MS Thomas, W Redmon, JB Raatz, SK AF Young, Lindsay R. Kurzer, Mindy S. Thomas, William Redmon, J. Bruce Raatz, Susan K. TI Low-fat diet with omega-3 fatty acids increases plasma insulin-like growth factor concentration in healthy postmenopausal women SO NUTRITION RESEARCH LA English DT Article DE Diet; Dietary fat; Omega-3 fatty acids; Insulin-like growth factor I (IGF-I); IGF binding protein-3 (IGFBP-3); Insulin; Glucose ID FACTOR BINDING-PROTEIN-3 CONCENTRATIONS; DIANA RANDOMIZED-TRIAL; IGF-BINDING PROTEIN-3; HIGH-FIBER DIET; FACTOR-I; SERUM CONCENTRATIONS; HORMONE REPLACEMENT; HIGH-CARBOHYDRATE; CANCER-RISK; SOY PROTEIN AB The insulin-like growth factor pathway plays a central role in the normal and abnormal growth of tissues; however, nutritional determinants of insulin-like growth factor I (IGF-I) and its binding proteins in healthy individuals are not well defined. Three test diets-high-fat diet (40% energy as fat), low-fat diet (LF; 20% energy as fat), and a diet with low fat and high omega-3 fatty acid (LFn3; 23% energy as fat)-were tested in a randomized crossover designed controlled feeding trial in healthy postmenopausal women. Plasma IGF-I, IGF binding protein-3 (IGFBP-3), insulin, glucose, and ratio of IGF-I/IGFBP-3 concentrations were measured in response to diets. Insulin sensitivity was calculated using the homeostatic model assessment of insulin resistance We hypothesized that IGF-I, insulin, and glucose concentrations would decrease and IGFBP-3 concentration would increase in response to the low-fat diets. Eight weeks of the LFn3 diet increased circulating IGF-I (P <.001) and IGFBP-3 (P =.01) and the LF diet increased IGFBP-3 (P =.04), resulting in trends toward an increased IGF-I/IGFBP-3 ratio with the LFn3 diet and a decreased IGF-I/IGFBP-3 ratio with the LF diet (P =.13 for both comparisons). No statistically significant differences were detected between treatments at baseline or 8 weeks for IGF-1, IGFBP-3, or the ratio of IGF-1/IGFBP-3. Insulin, glucose, and the homeostatic model assessment of insulin resistance were not altered by the interventions. Low-fat diet with high n-3 fatty acids may increase circulating IGF-I concentrations without adversely affecting insulin sensitivity in healthy individuals. Published by Elsevier Inc. C1 [Young, Lindsay R.; Kurzer, Mindy S.; Raatz, Susan K.] Univ Minnesota, Dept Food Sci & Nutr, Minneapolis, MN 55455 USA. [Thomas, William] Dept Biostat, Minneapolis, MN USA. [Redmon, J. Bruce] Univ Minnesota, Dept Med, Minneapolis, MN 55455 USA. [Raatz, Susan K.] USDA, Grand Forks Human Nutr Ctr, Grand Forks, ND 58203 USA. RP Raatz, SK (reprint author), USDA, Grand Forks Human Nutr Ctr, Grand Forks, ND 58203 USA. EM Susan.Raatz@ars.usda.gov OI Redmon, J. Bruce/0000-0002-1883-9467 FU Department of Defense [W81XWH-04-1-0448, W81XWH-06-1-0778]; National Center for Research Resources, National Institutes of Health [Mo1-RR00400] FX We would like to thank the participants for their serious commitment to this study and the General Clinical Research Center staff for their assistance with clinical work and preparation of the study diets. We thank Douglas Yee for his comments and review of the manuscript. Funding for this work was provided by grants from the Department of Defense (W81XWH-04-1-0448 and W81XWH-06-1-0778) and the National Center for Research Resources, National Institutes of Health (Mo1-RR00400). Salmon of the Americas, Inc, donated salmon fillets for the controlled diets. The authors declare no conflict of interest. NR 43 TC 6 Z9 6 U1 1 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0271-5317 J9 NUTR RES JI Nutr. Res. PD JUL PY 2013 VL 33 IS 7 BP 565 EP 571 DI 10.1016/j.nutres.2013.04.011 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 184IC UT WOS:000321882300007 PM 23827131 ER PT J AU Postnikova, OA Shao, J Nemchinov, LG AF Postnikova, Olga A. Shao, Jonathan Nemchinov, Lev G. TI Analysis of the Alfalfa Root Transcriptome in Response to Salinity Stress SO PLANT AND CELL PHYSIOLOGY LA English DT Article DE Medicago sativa; Next-generation sequencing; Root transcriptome; Salt stress ID SALT TOLERANCE; MEDICAGO-TRUNCATULA; EXPRESSION ANALYSIS; SATIVA L.; ARABIDOPSIS; GENE; PLANTS; GENOMICS; GROWTH; OVEREXPRESSION AB Salinity is one of the major abiotic factors affecting alfalfa productivity. Identifying genes that control this complex trait will provide critical insights for alfalfa breeding programs. To date, no studies have been published on a deep sequencing-based profiling of the alfalfa transcriptome in response to salinity stress. Observations gathered through research on reference genomes may not always be applicable to alfalfa. In this work, Illumina RNA-sequencing was performed in two alfalfa genotypes contrasting in salt tolerance, in order to estimate a broad spectrum of genes affected by salt stress. A total of 367,619,586 short reads were generated from cDNA libraries originated from roots of both lines. More than 60,000 tentative consensus sequences (TCs) were obtained and, among them, 74.5% had a significant similarity to proteins in the NCBI database. Mining of simple sequence repeats (SSRs) from all TCs revealed 6,496 SSRs belonging to 3,183 annotated unigenes. Bioinformatics analysis showed that the expression of 1,165 genes, including 86 transcription factors (TFs), was significantly altered under salt stress. About 40% of differentially expressed genes were assigned to known gene ontology (GO) categories using Arabidopsis GO. A random check of differentially expressed genes by quantitative real-time PCR confirmed the bioinformatic analysis of the RNA-seq data. A number of salt-responsive genes in both tested genotypes were identified and assigned to functional classes, and gene candidates with roles in the adaptation to salinity were proposed. Alfalfa-specific data on salt-responsive genes obtained in this work will be useful in understanding the molecular mechanisms of salinity tolerance in alfalfa. C1 [Postnikova, Olga A.; Shao, Jonathan; Nemchinov, Lev G.] ARS, USDA, Inst Plant Sci, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. RP Nemchinov, LG (reprint author), ARS, USDA, Inst Plant Sci, Mol Plant Pathol Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Lev.Nemchinov@ars.usda.gov FU United States Department of Agriculture, Agricultural Research Service FX This work was supported by the United States Department of Agriculture, Agricultural Research Service. NR 65 TC 39 Z9 40 U1 9 U2 92 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-0781 EI 1471-9053 J9 PLANT CELL PHYSIOL JI Plant Cell Physiol. PD JUL PY 2013 VL 54 IS 7 BP 1041 EP 1055 DI 10.1093/pcp/pct056 PG 15 WC Plant Sciences; Cell Biology SC Plant Sciences; Cell Biology GA 178QA UT WOS:000321461300002 PM 23592587 ER PT J AU Chao, WS Serpe, M Suttle, JC Jia, Y AF Chao, Wun S. Serpe, Marcelo Suttle, Jeffrey C. Jia, Ying TI Increase in ACC oxidase levels and activities during paradormancy release of leafy spurge (Euphorbia esula) buds SO PLANTA LA English DT Article DE ACC oxidase; Dormancy; Ethylene; Euphorbia; Leafy spurge; Paradormancy release; Underground adventitious buds ID 1-AMINOCYCLOPROPANE-1-CARBOXYLIC ACID OXIDASE; ETHYLENE PRODUCTION; DIFFERENTIAL EXPRESSION; GROWTH-INHIBITION; DORMANCY STATUS; ABSCISIC-ACID; WEED BIOLOGY; APPLE FRUIT; CROWN BUDS; RESPONSES AB The plant hormone ethylene is known to affect various developmental processes including dormancy and growth. Yet, little information is available about the role of ethylene during paradormancy release in underground adventitious buds of leafy spurge. In this study, we examined changes in ethylene evolution and the ethylene biosynthetic enzyme ACC oxidase following paradormancy release (growth induction). Our results did not show an obvious increase in ethylene during bud growth. However, when buds were incubated with 1 mM ACC, ethylene levels were higher in growing than non-growing buds, suggesting that the levels of ACC oxidase increased in growing buds. Real-time qPCR indicated that the transcript of a Euphorbia esula ACC oxidase (Ee-ACO) increased up to threefold following growth induction. In addition, a 2.5- to 4-fold increase in ACO activity was observed 4 days after decapitation, and the Ee-ACO accounted for 40 % of the total ACO activity. Immunoblot analyses identified a 36-kD Ee-ACO protein that increased in expression during bud growth. This protein was highly expressed in leaves, moderately expressed in crown buds, stems and meristems, and weakly expressed in roots and flowers. Immunolocalization of Ee-ACO on growing bud sections revealed strong labeling of the nucleus and cytoplasm in cells at the shoot apical meristem and leaf primordia. An exception to this pattern occurred in cells undergoing mitosis, where labeling of Ee-ACO was negligible. Taken together, our results indicated an increase in the levels of Ee-ACO during paradormancy release of leafy spurge that was not correlated with an increase in ethylene synthesis. C1 [Chao, Wun S.; Suttle, Jeffrey C.] ARS, USDA, Fargo, ND 58102 USA. [Serpe, Marcelo] Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA. [Jia, Ying] Texas A&M Univ Kingsville, Natl Nat Toxins Res Ctr, Kingsville, TX 78363 USA. RP Chao, WS (reprint author), ARS, USDA, Fargo, ND 58102 USA. EM wun.chao@ars.usda.gov NR 47 TC 0 Z9 0 U1 3 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 J9 PLANTA JI Planta PD JUL PY 2013 VL 238 IS 1 BP 205 EP 215 DI 10.1007/s00425-013-1887-z PG 11 WC Plant Sciences SC Plant Sciences GA 171WX UT WOS:000320961900014 PM 23625016 ER PT J AU Yildiz, M Willis, DK Cavagnaro, PF Iorizzo, M Abak, K Simon, PW AF Yildiz, Mehtap Willis, David K. Cavagnaro, Pablo F. Iorizzo, Massimo Abak, Kazim Simon, Philipp W. TI Expression and mapping of anthocyanin biosynthesis genes in carrot SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID DAUCUS-CAROTA; PETUNIA-HYBRIDA; SWEET-POTATO; TARGET GENES; MARKERS; PURPLE; SYNTHASE; PATHWAY; ROOTS; COLOR AB Anthocyanin gene expression has been extensively studied in leaves, fruits and flowers of numerous plants. Little, however, is known about anthocyanin accumulation in roots of carrots or other species. We quantified expression of six anthocyanin biosynthetic genes [phenylalanine ammonia-lyase (PAL3), chalcone synthase (CHS1), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR1), leucoanthocyanidin dioxygenase (LDOX2), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT)] in three carrot inbreds with contrasting root color: solid purple (phloem and xylem); purple outer phloem/orange xylem; and orange phloem and xylem. Transcripts for five of these genes (CHS1, DFR1, F3H, LDOX2, PAL3) accumulated at high levels in solid purple carrots, less in purple-orange carrot, and low or no transcript in orange carrots. Gene expression coincided with anthocyanin accumulation. In contrast, UFGT expression was comparable in purple and orange carrots and relatively unchanged during root development. In addition, five anthocyanin biosynthesis genes [FLS1 (flavonol synthase), F3H, LDOX2, PAL3, and UFGT] and three anthocyanin transcription factors (DcEFR1, DcMYB3 and DcMYB5) were mapped in a population segregating for the P (1) locus that conditions purple root color. P (1) mapped to chromosome 3 and of the eight anthocyanin biosynthesis genes, only F3H and FLS1 were linked to P (1). The gene expression and mapping data suggest a coordinated regulatory control of anthocyanin expression in carrot root and establish a framework for studying the anthocyanin pathway in carrots, and they also suggest that none of the genes evaluated is a candidate for P (1). C1 [Yildiz, Mehtap; Cavagnaro, Pablo F.; Iorizzo, Massimo; Simon, Philipp W.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Yildiz, Mehtap] Yuzuncu Yil Univ, Fac Agr, Dept Agr Biotechnol, TR-65080 Van, Turkey. [Willis, David K.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Willis, David K.; Simon, Philipp W.] Univ Wisconsin, USDA, ARS, Vegetable Crops Unit, Madison, WI 53706 USA. [Cavagnaro, Pablo F.] Inst Nacl Tecnol Agr, Estn Expt Agr La Consulta, RA-5567 Mendoza, Argentina. [Cavagnaro, Pablo F.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Abak, Kazim] Lefke European Univ, TRNC, Fac Agr Sci & Technol, Dept Hort Prod & Mkt, TR-10 Mersin, Turkey. RP Simon, PW (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM psimon@wisc.edu FU Initiative for Future Agriculture Food Systems from the USDA Cooperative Research, Education, and Extension Service, USDA, ARS [2000-4258]; University of Wisconsin-Madison; California Fresh Carrot Advisory Board FX This article includes parts of the doctoral thesis of the first author. The authors gratefully acknowledge support from the Initiative for Future Agriculture Food Systems Grant number 2000-4258 from the USDA Cooperative Research, Education, and Extension Service, the USDA, ARS; the University of Wisconsin-Madison; the California Fresh Carrot Advisory Board; and vegetable seed companies for their support of this research. The authors are also grateful for the proficient technical assistance of Drs. Megan Bowman and Douglas Senalik. NR 56 TC 11 Z9 13 U1 2 U2 86 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JUL PY 2013 VL 126 IS 7 BP 1689 EP 1702 DI 10.1007/s00122-013-2084-y PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA 170PT UT WOS:000320866200001 PM 23525633 ER PT J AU Liu, RC Finkelman, BS Chen, JB Booth, SL Bershaw, L Brensinger, C Kimmel, SE AF Liu, Ron C. Finkelman, Brian S. Chen, Jinbo Booth, Sarah L. Bershaw, Luanne Brensinger, Colleen Kimmel, Stephen E. TI Dietary vitamin K intake and anticoagulation control during the initiation phase of warfarin therapy: A prospective cohort study SO THROMBOSIS AND HAEMOSTASIS LA English DT Letter ID VARIABILITY; MANAGEMENT C1 [Liu, Ron C.; Finkelman, Brian S.; Chen, Jinbo; Bershaw, Luanne; Brensinger, Colleen; Kimmel, Stephen E.] Univ Penn, Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [Liu, Ron C.; Finkelman, Brian S.; Chen, Jinbo; Bershaw, Luanne; Brensinger, Colleen; Kimmel, Stephen E.] Univ Penn, Perelman Sch Med, Ctr Clin Epidemiol & Biostat, Philadelphia, PA 19104 USA. [Finkelman, Brian S.; Bershaw, Luanne; Kimmel, Stephen E.] Univ Penn, Perelman Sch Med, Ctr Therapeut Effectiveness Res, Philadelphia, PA 19104 USA. [Booth, Sarah L.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Philadelphia, PA USA. [Kimmel, Stephen E.] Univ Penn, Perelman Sch Med, Dept Med, Philadelphia, PA 19104 USA. [Liu, Ron C.] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. RP Kimmel, SE (reprint author), Univ Penn, Perelman Sch Med, Ctr Clin Epidemiol & Biostat, 923 Blockley Hall,423 Guardian Dr, Philadelphia, PA 19104 USA. EM stevek@mail.med.upenn.edu OI Finkelman, Brian/0000-0002-4348-8208 NR 13 TC 2 Z9 2 U1 0 U2 3 PU SCHATTAUER GMBH-VERLAG MEDIZIN NATURWISSENSCHAFTEN PI STUTTGART PA HOLDERLINSTRASSE 3, D-70174 STUTTGART, GERMANY SN 0340-6245 J9 THROMB HAEMOSTASIS JI Thromb. Haemost. PD JUL PY 2013 VL 110 IS 1 BP 195 EP 196 DI 10.1160/TH13-02-0111 PG 2 WC Hematology; Peripheral Vascular Disease SC Hematology; Cardiovascular System & Cardiology GA 178VP UT WOS:000321475800027 ER PT J AU Karl, JW Gillan, JK Herrick, JE AF Karl, Jason W. Gillan, Jeffrey K. Herrick, Jeffrey E. TI Geographic searching for ecological studies: a new frontier SO TRENDS IN ECOLOGY & EVOLUTION LA English DT Letter C1 [Karl, Jason W.; Herrick, Jeffrey E.] New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. [Gillan, Jeffrey K.] New Mexico State Univ, Las Cruces, NM 88003 USA. RP Karl, JW (reprint author), New Mexico State Univ, USDA ARS, Las Cruces, NM 88003 USA. EM jkarl@nmsu.edu OI Karl, Jason/0000-0002-3326-3806 NR 10 TC 4 Z9 4 U1 0 U2 20 PU ELSEVIER SCIENCE LONDON PI LONDON PA 84 THEOBALDS RD, LONDON WC1X 8RR, ENGLAND SN 0169-5347 J9 TRENDS ECOL EVOL JI Trends Ecol. Evol. PD JUL PY 2013 VL 28 IS 7 BP 383 EP 384 DI 10.1016/j.tree.2013.05.001 PG 2 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 185VE UT WOS:000321997600003 PM 23706558 ER PT J AU Dubey, JP Choudhary, S Kwok, OCH Ferreira, LR Oliveira, S Verma, SK Marks, DR Pedersen, K Mickley, RM Randall, AR Arsnoe, D Su, C AF Dubey, J. P. Choudhary, S. Kwok, O. C. H. Ferreira, L. R. Oliveira, S. Verma, S. K. Marks, D. R. Pedersen, K. Mickley, R. M. Randall, A. R. Arsnoe, D. Su, C. TI Isolation and genetic characterization of Toxoplasma gondii from mute swan (Cygnus olor) from the USA SO VETERINARY PARASITOLOGY LA English DT Article DE Toxoplasma gondii; Mute swan; Cygnus olor; Wildlife; Seroprevalence; Isolation; Genetic types; USA ID HIGH PREVALENCE; CONGENITAL TOXOPLASMOSIS; HUMAN CONSUMPTION; UNITED-STATES; GENOTYPES; IDENTIFICATION; LINEAGES; OUTBREAK; ANIMALS; DISEASE AB Little is known of the genetic diversity of Toxoplasma gondii circulating in wildlife. In the present study, antibodies to T. gondii were determined in serum samples from 632 mute swans (Cygnus olor) collected from different areas of the USA. Sera were tested by T. gondii modified agglutination test; 54 (8.5%) of 632 samples were seropositive with titers of 25 in 28 sera, 50 in 22 sera, 100 in three samples, and 200 or higher in one swan. Hearts from 14 seropositive swans were bioassayed in mice and viable T. gondii (designated TgSwanUs1-3) were isolated from the hearts of three. These three T. gondii isolates were further propagated in cell culture, and DNA isolated from culture-derived tachyzoites was characterized using 11 PCR-RFLP markers (SAG1, 5'- and 3'-SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico). Results of genotyping revealed that two strains (TgSwanUs1, TgSwanUs2) were Type III (ToxoDB PCR-RFLP genotype #2), and TgSwanUs3 was a new genotype designated here as ToxoDB PCR-RFLP genotype #216. Pathogenicity of oocysts derived from these three strains was determined in Swiss Webster (SW) outbred mice. All mice infected with oocysts and tachyzoites of the atypical isolate (TgSwanUs3) died of acute toxoplasmosis, irrespective of the dose. Oocysts of the remaining two isolates were. less pathogenic but differed from each other; 10 oocysts of the TgSwanUs1 killed all inoculated mice whereas 1 million oocysts of the TgSwanUs2 were needed to kill all infected SW mice. Isolation of T. gondii from mute swan indicates that the local waters were contaminated by T. gondii oocysts, and that mouse T. gondii virulent strains are circulating in wildlife. Mute swan is a new host record for T. gondii. Published by Elsevier B.V. C1 [Dubey, J. P.; Choudhary, S.; Kwok, O. C. H.; Ferreira, L. R.; Oliveira, S.; Verma, S. K.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Marks, D. R.; Arsnoe, D.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Okemos, MI 48864 USA. [Pedersen, K.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Ft Collins, CO 80521 USA. [Mickley, R. M.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Sutton, MA 01590 USA. [Randall, A. R.] Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Pittstown, NJ 08867 USA. [Su, C.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov RI Su, Chunlei/M-1892-2013; Oliveira , Solange/B-7544-2014 OI Su, Chunlei/0000-0001-8392-7108; FU United States Fish and Wildlife Service as part of their Avian Health and Disease Program [APHIS-WS: 12-7226-4861-IA] FX Funding for a portion of the field sample collection effort was provided by the United States Fish and Wildlife Service, through an interagency agreement (APHIS-WS: 12-7226-4861-IA) as part of their Avian Health and Disease Program. NR 32 TC 8 Z9 8 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 J9 VET PARASITOL JI Vet. Parasitol. PD JUL 1 PY 2013 VL 195 IS 1-2 BP 42 EP 46 DI 10.1016/j.vetpar.2012.12.051 PG 5 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA 168VR UT WOS:000320736500005 PM 23394800 ER PT J AU Nandula, VK Ray, JD Ribeiro, DN Pan, Z Reddy, KN AF Nandula, Vijay K. Ray, Jeffery D. Ribeiro, Daniela N. Pan, Z. Reddy, Krishna N. TI Glyphosate Resistance in Tall Waterhemp (Amaranthus tuberculatus) from Mississippi is due to both Altered Target-Site and Nontarget-Site Mechanisms SO WEED SCIENCE LA English DT Article DE Absorption; autoradiography; EPSPS; gene amplification; mutation; phosphorimaging; shikimate; translocation ID RYEGRASS LOLIUM-MULTIFLORUM; MULTIPLE HERBICIDE RESISTANCE; HORSEWEED CONYZA-CANADENSIS; ELEUSINE-INDICA; GENE AMPLIFICATION; RIGIDUM POPULATION; FIELD CONDITIONS; TRANSLOCATION; MUTATION; CALIFORNIA AB A tall waterhemp population from Missisippi was suspected to be resistant to glyphosate. Glyphosate dose response experiments resulted in GR(50) (dose required to reduce plant growth by 50%) values of 1.28 and 0.28 kg ae ha(-1) glyphosate for the glyphosate-resistant (GR) and -susceptible (GS) populations, respectively, indicating a five-fold resistance. The absorption pattern of C-14-glyphosate between the GR and GS populations was similar up to 24 h after treatment (HAT). Thereafter, the susceptible population absorbed more glyphosate (55 and 49% of applied) compared to the resistant population (41 and 40% of applied) by 48 and 72 HAT, respectively. Treatment of a single leaf in individual plants with glyphosate at 0.84 kg ha(-1), in the form of 10 1-mu l droplets, provided greater control (85 vs. 29%) and shoot fresh weight reduction (73 vs. 34% of nontreated control) of the GS plants compared to the GR plants, possibly indicating a reduced movement of glyphosate in the GR plants. The amount of C-14-glyphosate that translocated out of the treated leaves of GR plants (20% of absorbed at 24 HAT and 23% of absorbed at 48 HAT) was significantly lower than the GS plants (31% of absorbed at 24 HAT and 32% of absorbed at 48 HAT). A potential difference in shikimate accumulation between GR and GS populations at different concentrations of glyphosate was also studied in vitro. The IC50 (glyphosate concentration required to cause shikimate accumulation at 50% of peak levels measured) values for the GR and GS populations were 480 and 140 mu M of glyphosate, respectively, resulting in more shikimate accumulation in the GS than the GR population. Sequence analysis of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), the target site of glyphosate, from GR and GS plants identified a consistent single nucleotide polymorphism (T/C, thymine/cytosine) between GR/GS plants, resulting in a proline to serine amino acid substitution at position 106 in the GR population. The GR and GS plants contained equal genomic copy number of EPSPS, which was positively correlated with EPSPS gene expression. Thus, glyphosate resistance in the tall waterhemp population from Mississippi is due to both altered target site and nontarget site mechanisms. This is the first report of an altered EPSPS-based resistance in a dicot weed species that has evolved resistance to glyphosate. C1 [Nandula, Vijay K.; Reddy, Krishna N.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. [Ray, Jeffery D.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38766 USA. [Ribeiro, Daniela N.] Mississippi State Univ, Dept Crop & Soil Sci, Mississippi State, MS 39762 USA. [Pan, Z.] USDA ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. RP Nandula, VK (reprint author), USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. EM vijay.nandula@ars.usda.gov FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS) [6402-21000-050-00D, 6402-21220-010-00D] FX The technical assistance of Nikhil Chatakondi, Angelie Davis, and Earl Gordon is very much appreciated. This work was supported by the United States Department of Agriculture-Agricultural Research Service (USDA-ARS) project numbers 6402-21000-050-00D and 6402-21220-010-00D NR 48 TC 22 Z9 22 U1 0 U2 44 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD JUL-SEP PY 2013 VL 61 IS 3 BP 374 EP 383 DI 10.1614/WS-D-12-00155.1 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 176QX UT WOS:000321320300005 ER PT J AU Davis, AS Clay, S Cardina, J Dille, A Forcella, F Lindquist, J Sprague, C AF Davis, Adam S. Clay, Sharon Cardina, John Dille, Anita Forcella, Frank Lindquist, John Sprague, Christy TI Seed Burial Physical Environment Explains Departures from Regional Hydrothermal Model of Giant Ragweed (Ambrosia trifida) Seedling Emergence in US Midwest SO WEED SCIENCE LA English DT Article DE Abiotic influences on seed dormancy; hydrothermal time; nonlinear mixed effects models; regional environmental variation; seedling recruitment phenology ID CORN ZEA-MAYS; WEED EMERGENCE; CHENOPODIUM-ALBUM; GERMINATION; DORMANCY; TEMPERATURE; GROWTH; SIMULATION; MANAGEMENT; TILLAGE AB Robust predictions of weed seedling emergence from the soil seedbank are needed to aid weed management. A common seed accession (Illinois) of giant ragweed was buried in replicate experimental gardens over 18 site years in Illinois, Michigan, Kansas, Nebraska, Ohio, and South Dakota to examine the importance of site and climate variability by year on seedling emergence. In a nonlinear mixed-effects modeling approach, we used a flexible sigmoidal function (Weibull) to model giant ragweed cumulative seedling emergence in relation to hydrothermal time accumulated in each site-year. An iterative search method across a range of base temperature (T-b) and base and ceiling soil matric potentials (psi(b) and psi(c)) for accumulation of hydrothermal time identified optima (T-b = 4.4 C, psi(b) = 2,500 kPa, psi(c) = 0 kPa) that resulted in a parsimonious regional model. Deviations between the fits for individual site-years and the fixed effects regional model were characterized by a negative relationship between random effects for the shape parameter lrc (natural log of the rate constant, indicating the speed at which emergence progressed) and thermal time (base 10 C) during the seed burial period October through March (r = -0.51, P = 0.03). One possible implication of this result is that cold winter temperatures are required to break dormancy in giant ragweed seeds. By taking advantage of advances in statistical computing approaches, development of robust regional models now is possible for explaining arable weed seedling emergence progress across wide regions. C1 [Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Clay, Sharon] S Dakota State Univ, Brookings, SD 57007 USA. [Cardina, John] Ohio State Univ, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Dille, Anita] Kansas State Univ, Manhattan, KS 66506 USA. [Forcella, Frank] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Lindquist, John] Univ Nebraska, Lincoln, NE 68583 USA. [Sprague, Christy] Michigan State Univ, E Lansing, MI 48824 USA. RP Davis, AS (reprint author), USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. EM adam.davis@ars.usda.gov FU NCRA(North Central Regional Association of agricultural experiment station directors) [NC-1026]; Kansas State University; Michigan State University; University of Nebraska; Ohio State University; South Dakota State University; USDA-ARS FX This work was supported by NCRA(North Central Regional Association of agricultural experiment station directors)project NC-1026, Kansas State University, Michigan State University, University of Nebraska, The Ohio State University, South Dakota State University, and USDA-ARS. We express our sincere thanks to the many research assistants and technicians who helped make this study possible. NR 34 TC 10 Z9 10 U1 2 U2 23 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD JUL-SEP PY 2013 VL 61 IS 3 BP 415 EP 421 DI 10.1614/WS-D-12-00139.1 PG 7 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 176QX UT WOS:000321320300011 ER PT J AU Horvath, DP Santana, MA Anderson, JV AF Horvath, David P. Santana, Maria A. Anderson, James V. TI Microarray Analysis of the Semicompatible, Pathogenic Response and Recovery of Leafy Spurge (Euphorbia esula) Inoculated with the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis SO WEED SCIENCE LA English DT Article DE Network analysis; pathogenesis; transcriptomics ID GTP-BINDING PROTEIN; GENE-EXPRESSION; SALICYLIC-ACID; RESISTANCE RESPONSE; ARABIDOPSIS MUTANT; DEFENSE RESPONSES; TOBACCO PLANTS; INFECTION; DISEASE; TOMATO AB Infection by Xanthomonas axonopodis pv. manihotis (Xam) of the perennial rangeland weed leafy spurge was tested to see whether Xam might serve a potential biological control agent for this invasive weed. Although leafy spurge was susceptible to Xam infection, it recovered within 21 d after inoculation (DAI). Microarray resources available for leafy spurge allowed us to follow the physiological and signaling pathways that were altered as leafy spurge was infected and then recovered from Xam infection. The first physiological effect of Xam infection was a down-regulation of photosynthetic processes within 1 DAI. By 7 DAI, numerous processes associated with well-documented pathogenesis responses of plants were observed. Although some pathogenesis responses were still detectable at 21 DAI, other processes associated with meristem development were noted. Ontological analysis of potential signaling systems indicated jasmonic acid plays a significant role in the recovery processes. C1 [Horvath, David P.; Anderson, James V.] USDA ARS, Sunflower & Plant Biol Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. [Santana, Maria A.] Univ Simon Bolivar, Div Ciencias Biol, Dept Biol Celular, Caracas, Venezuela. [Santana, Maria A.] Ctr Biotecnol, Inst Estudios Avanzados, Caracas, Venezuela. RP Horvath, DP (reprint author), USDA ARS, Sunflower & Plant Biol Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. EM David.horvath@ars.usda.gov NR 51 TC 0 Z9 0 U1 1 U2 6 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD JUL-SEP PY 2013 VL 61 IS 3 BP 428 EP 436 DI 10.1614/WS-D-12-00177.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 176QX UT WOS:000321320300013 ER PT J AU Williams, MM Boydston, RA AF Williams, Martin M., II Boydston, Rick A. TI Crop Seeding Level: Implications for Weed Management in Sweet Corn SO WEED SCIENCE LA English DT Article DE Competition; germinability; plant population; planting arrangement; seed bank; seed production; seeding density ID MILLET PANICUM-MILIACEUM; ZEA-MAYS; SUPPRESSIVE ABILITY; POPULATION DENSITY; PLANT-POPULATION; MAIZE; YIELD; WILD; COMPETITION; INTERFERENCE AB Sweet corn is seeded under a wide range of population densities; however, the extent to which variable population density influences weed suppression is unknown. Therefore, field studies were undertaken to quantify the influence of sweet corn seeding level on growth, seed production, and post-harvest seed germination of wild-proso millet, one of the most problematic weeds in the crop. As crop seeding level increased, path analysis results indicated the crop canopy became taller and thicker, resulting in less wild-proso millet biomass, seed production, and germinability. However, at the level of individual fields, reductions in wild-proso millet growth and seed production were modest, at best, between a crop population currently used by growers and a higher crop population known to optimize yield of certain hybrids. These results indicate near-future increases in sweet corn seeding levels may play a minor role in improving weed management in individual sweet corn fields. Nonetheless, a reduction in crop populations, via weather- or management-driven phenomenon, increases risk of greater wild-proso millet seed production. C1 [Williams, Martin M., II] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Boydston, Rick A.] USDA ARS, Prosser, WA 99350 USA. RP Williams, MM (reprint author), Univ Illinois, USDA ARS, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM mmwillms@illinois.edu NR 30 TC 0 Z9 0 U1 1 U2 19 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD JUL-SEP PY 2013 VL 61 IS 3 BP 437 EP 442 DI 10.1614/WS-D-12-00205.1 PG 6 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 176QX UT WOS:000321320300014 ER PT J AU Dalley, CD Viator, RP Richard, EP AF Dalley, Caleb D. Viator, Ryan P. Richard, Edward P., Jr. TI Integrated Management of Bermudagrass (Cynodon dactylon) in Sugarcane SO WEED SCIENCE LA English DT Article DE Banded herbicide application; postharvest crop residue; tillage ID JOHNSONGRASS SORGHUM-HALEPENSE; REDUCED-TILLAGE PROGRAMS; SACCHARUM SPP. HYBRIDS; WEED-CONTROL; RESIDUE MANAGEMENT; SOIL PROPERTIES; SUMMER FALLOW; INTERFERENCE; STRAW; CORN AB Bermudagrass is a difficult perennial weed to manage in Louisiana sugarcane. Research was conducted to compare interrow tillage practice, postharvest residue management, and herbicide placement on bermudagrass proliferation and sugarcane yield. Tillage frequencies included conventional (four tillage operations per season), reduced (two tillage operations), and no-till. Residue management practices included removal by burning, sweeping from row top into the wheel furrow, and not removed. Spring herbicide placement treatments included broadcast, banded, or no herbicide application. With conventional tillage, broadcast and banded herbicide applications resulted in similar bermudagrass cover in the First and second ratoon crops, but bermudagrass cover was greater when using banded applications (22%) compared with broadcast application (15%) in the third-ratoon crop. Bermudagrass cover was greatest with no-till. When herbicides were banded, bermudagrass cover was greater in reduced tillage than conventional tillage in all three ratoon crops. Postharvest residue management did not affect bermudagrass ground cover. In plant cane, sugarcane yields were lowest when herbicide was not applied. In ratoon crops, sugarcane and sugar yield were reduced when herbicide was not applied regardless of tillage practice. Cane and sugar yield were generally equal when comparing reduced and conventional tillage. Total sugarcane yield (4 crop yr) for the no-till program was reduced 11, 15, and 25%, respectively, when herbicides were broadcast, banded, and when herbicide was not applied, compared with conventional tillage. Failure to remove residue reduced sugarcane yield by 5, 7, and 10% in first, second, and third ratoons, respectively, compared with burning. Eliminating unnecessary tillage practices can increase profitability of sugarcane through reduced costs, but it will be imperative that herbicide programs be included to provide adequate bermudagrass control and that postharvest residue is removed to promote maximum sugarpostane yield. C1 [Dalley, Caleb D.; Viator, Ryan P.; Richard, Edward P., Jr.] USDA ARS, Sugarcane Res Unit, Houma, LA 70360 USA. RP Dalley, CD (reprint author), USDA ARS, Sugarcane Res Unit, 5883 USDA Rd, Houma, LA 70360 USA. EM caleb.dalley@ars.usda.gov NR 41 TC 1 Z9 1 U1 1 U2 11 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 J9 WEED SCI JI Weed Sci. PD JUL-SEP PY 2013 VL 61 IS 3 BP 482 EP 490 DI 10.1614/WS-D-12-00124.1 PG 9 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA 176QX UT WOS:000321320300019 ER PT J AU Lutes, DC Hardy, CC AF Lutes, Duncan C. Hardy, Colin C. TI Lodgepole Pine Bole Wood Density 1 and 11 Years after Felling in Central Montana SO WESTERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE biomass; decomposition; decay class; decay rate; down woody material ID NUTRIENT DYNAMICS; NATIONAL-PARK; DEBRIS; FORESTS; OREGON; DECAY; DECOMPOSITION; ECOSYSTEMS; MASS; LATIFOLIA AB Estimates of large dead and down woody material biomass are used for evaluating ecological processes and making ecological assessments, such as for nutrient cycling, wildlife habitat, fire effects, and climate change science. Many methods are used to assess the abundance (volume) of woody material, which ultimately require an estimate of wood density to convert volume to biomass. To assess wood density and decomposition rate, this study examined in situ wood density of lodgepole pine logs at the Tenderfoot Creek Experimental Forest, central Montana, 1 and 11 years after felling. Wood density decreased from 0.39 g cm(-3) to 0.35 g cm(-3) over 10 years and the single exponential decay rate was 0.0085 yr(-1). A common 5-category decay classification system was evaluated for estimating wood density by decay class; however, the relationship was only partially significant. C1 [Lutes, Duncan C.; Hardy, Colin C.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT USA. RP Lutes, DC (reprint author), US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT USA. EM dlutes@fs.fed.us; chardy01@fs.fed.us NR 35 TC 1 Z9 1 U1 2 U2 6 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0885-6095 J9 WEST J APPL FOR JI West. J. Appl. For. PD JUL PY 2013 VL 28 IS 3 BP 116 EP 120 DI 10.5849/wjaf.12-033 PG 5 WC Forestry SC Forestry GA 182EN UT WOS:000321724600004 ER PT J AU Brodie, LC DeBell, DS AF Brodie, Leslie Chandler DeBell, Dean S. TI Residual Densities Affect Growth of Overstory Trees and Planted Douglas-Fir, Western Hemlock, and Western Redcedar: Results from the First Decade SO WESTERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE overstory retention; regeneration; Pseudotsuga menziesii; Tsuga heterophylla; Thuja plicata ID OREGON COAST RANGE; SILVICULTURAL SYSTEMS; BRITISH-COLUMBIA; STANDS; REGENERATION; RETENTION; FORESTS; SEEDLINGS; SURVIVAL AB In recent years, interest has increased in silvicultural systems and harvest cuts that retain partial overstories, but there are few data available on the growth of the understory trees in such stands. We studied the response of overstory trees and underplanted seedlings, Douglas-fir (Pseudotsuga menziesii), western hemlock (Tsuga heterophylla), and western redcedar (Thuja plicata), to a range of residual overstory densities. Forty to 70-year-old Douglas-fir stands in western Washington were harvested, leaving retention levels of 0, 8, 16, 24, 32, and 40% of full stocking. The 9-year response of the understory seedlings was species-dependent with Douglas-fir the largest in diameter (mean diameter 6.4 cm and mean height 3.8 m), western hemlock the tallest (mean diameter 5.5 cm and mean height 5.4 m), and redcedar the smallest (mean diameter 1.5 cm and mean height 1.5 m), in part because it was heavily browsed. Douglas-fir and western redcedar showed the greatest growth in the lowest retention levels (0 and 8%), and western hemlock responded best at the middle retention levels (8 and 16%). C1 [Brodie, Leslie Chandler; DeBell, Dean S.] US Forest Serv, Pacific NW Res Stn, USDA, Olympia, WA 98502 USA. RP Brodie, LC (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, Olympia, WA 98502 USA. EM lbrodie@fs.fed.us; djdebell@comcast.net NR 35 TC 2 Z9 2 U1 1 U2 14 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0885-6095 J9 WEST J APPL FOR JI West. J. Appl. For. PD JUL PY 2013 VL 28 IS 3 BP 121 EP 127 DI 10.5849/wjaf.12-019 PG 7 WC Forestry SC Forestry GA 182EN UT WOS:000321724600005 ER PT J AU Thies, WG Westlind, DJ Loewen, M AF Thies, Walter G. Westlind, Douglas J. Loewen, Mark TI Impact of Spring or Fall Repeated Prescribed Fire on Growth of Ponderosa Pine in Eastern Oregon, USA SO WESTERN JOURNAL OF APPLIED FORESTRY LA English DT Article DE bark thickness; Blue Mountains; Pinus ponderosa ID FORESTS; SEASON; MORTALITY; RESPONSES; SEVERITY; ARIZONA; BURN AB Prescribed burning is used to reduce fuel loads and to return fire to its historic disturbance role in western forests. Managers need to know the effects of prescribed fire on tree growth. Growth of residual ponderosa pine (Pinus ponderosa Dougl. ex Laws.) was measured in an existing long-term study of the effects of season-of-prescribed burn in combination with a single or a repeated burn. Each of six previously thinned mixed-age stands in the Blue Mountains near Burns, Oregon, was subdivided into three experimental units, and one of three treatments was randomly assigned to each: fall 1997 burn, spring 1998 burn, and no burn (control). Treatments were operational-sized prescribed burns. In 2002/2003 each burned unit was split, and one-half was burned again, maintaining the original season of burn. Sample trees were evaluated for growth 5 and 10 seasons after the initial prescribed fires. We conclude that after accounting for the crown scorch proportion neither a single spring or fall prescribed fire treatment nor a repeated fire treatment 5 years later affected the growth of thinned ponderosa pine 10 years after the initial 1997/1998 fires. A linear regression is included to estimate bark thickness based on dbh. C1 [Thies, Walter G.] USDA, Forest Serv, Forestry Sci Lab, Corvallis, OR USA. [Westlind, Douglas J.; Loewen, Mark] USDA, Forest Serv, Corvallis, OR USA. RP Thies, WG (reprint author), USDA, Forest Serv, Forestry Sci Lab, Corvallis, OR USA. EM wgthies@fs.fed.us; dwestlind@fs.fed.us; mloewen@fs.fed.us FU Forest Health Protection of the US Department of Agriculture Forest Service through the Special Technology Development Program; Joint Fires Sciences Program [01B-3-3-16] FX We thank the Malheur National Forest and the Emigrant Creek Ranger District for their support throughout the planning and execution of this study. Without their constant and unselfish support, this project would not have been possible. Fieldwork was conducted with funds provided by Forest Health Protection of the US Department of Agriculture Forest Service through the Special Technology Development Program and with funds from the Joint Fires Sciences Program proposal 01B-3-3-16. We thank Roland (Buddy) Crisafi, Gene Mackey, Ken Higle, Kim Valentine, and Dave Hill for supervising the conduct of the prescribed burns and Kevin Ryan fir discussions in the flea thanks also to crew leader Megan Pankey and the many members of our field crews who worked long and hard to establish the study and collect the necessary data. We thank Rick Kelsey, Paul Anderson, and Michelle Buonopane for comments, discussions, and reviews during the development of this article and several anonymous reviewers for well-reasoned and useful comments. NR 24 TC 2 Z9 2 U1 4 U2 18 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0885-6095 J9 WEST J APPL FOR JI West. J. Appl. For. PD JUL PY 2013 VL 28 IS 3 BP 128 EP 132 DI 10.5849/wjaf.11-044 PG 5 WC Forestry SC Forestry GA 182EN UT WOS:000321724600006 ER PT J AU Revay, EE Kline, DL Xue, RD Qualls, WA Bernier, UR Kravchenko, VD Ghattas, N Pstygo, I Muller, GC AF Revay, Edita E. Kline, Daniel L. Xue, Rui-De Qualls, Whitney A. Bernier, Ulrich R. Kravchenko, Vasiliy D. Ghattas, Nina Pstygo, Irina Mueller, Gunter C. TI Reduction of mosquito biting-pressure: Spatial repellents or mosquito traps? A field comparison of seven commercially available products in Israel SO ACTA TROPICA LA English DT Article DE Mosquito; Biting pressure; Spatial repellent; Mosquito trap; EPA 25b exempt; Terpenes; D-cis/trans allethrin ID ESSENTIAL OILS; INSECT REPELLENTS; VECTOR CONTROL; HOST-SEEKING; EFFICACY; AEDES; DEET; CITRONELLA; ANOPHELES; GERANIOL AB The present study assessed the personal protection efficiency of seven commercially available mosquito control devices (MCD) under field conditions in Israel. Trials were performed in a high biting-pressure area inhabited by large populations of mosquito and biting midge species, using human volunteers as bait in landing catch experiments. Results show that under minimal air-movement, three spatial repellent based products (ThermaCELL (R) Patio Lantern, OFFI (R) PowerPad lamp, and Terminix (R) ALLCLEAR Tabletop Mosquito Repeller) significantly reduced the biting-pressure (t-test - P < 0.01) when positioned at short distances from a volunteer (3, 7.5, and 10 ft.), with the ThermaCELL unit being most effective (96.1, 89.9, and 76.66% reduction, respectively). No significant differences were seen between the three aforementioned devices at distances of 3 and 7.5 ft., while at a distance of 10 ft., only the ThermaCELL patio lantern repelled significantly more mosquitoes then the Terminix ALLCLEAR Tabletop Mosquito Repeller (t-test, P < 0.05). In contrast, mosquito traps using attracting cues to bait mosquitoes (Dynatrap (R), Vortex (R) Electronic Insect Trap, Blue Rhino SV3100) either significantly increased or had no effect on the biting-pressure at short distances compared with the unprotected control. Trials conducted over large areas showed that only the Blue Rhino trap was able to significantly reduce the biting-pressure (40.1% reduction), but this was only when operating four units at the corners of an intermediate sized area. (c) 2013 Elsevier B.V. All rights reserved. C1 [Revay, Edita E.] Technion Israel Inst Technol, Bruce Rappaport Fac Med, Dept Anat & Cell Biol, IL-34995 Haifa, Israel. [Kline, Daniel L.; Bernier, Ulrich R.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Xue, Rui-De; Qualls, Whitney A.] Anastasia Mosquito Control Dist, St Augustine, FL 32080 USA. [Kravchenko, Vasiliy D.] Tel Aviv Univ, George S Wise Fac Life Sci, Dept Zool, IL-69978 Tel Aviv, Israel. [Ghattas, Nina] Natl Hlth Maintenance Org, IL-17000 Nazareth, Israel. [Pstygo, Irina] Ulyanovsk State Univ, Fac Foreign Languages & Profess Commun, Ulyanovsk 432017, Russia. [Mueller, Gunter C.] Hebrew Univ Jerusalem, Kuvin Ctr Study Infect & Trop Dis, Dept Microbiol & Mol Genet, IMRIC,Fac Med, IL-91120 Jerusalem, Israel. RP Muller, GC (reprint author), Hebrew Univ Jerusalem, Kuvin Ctr Study Infect & Trop Dis, IL-91120 Jerusalem, Israel. EM guntercmuller@hotmail.com NR 29 TC 15 Z9 15 U1 3 U2 34 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X J9 ACTA TROP JI Acta Trop. PD JUL PY 2013 VL 127 IS 1 BP 63 EP 68 DI 10.1016/j.actatropica.2013.03.011 PG 6 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA 152QH UT WOS:000319545700011 PM 23545129 ER PT J AU Gitz, DC Baker, JT Stout, JE Brauer, DK Lascano, RJ Velten, JP AF Gitz, Dennis C., III Baker, Jeffrey T. Stout, John E. Brauer, David K. Lascano, Robert J. Velten, Jeff P. TI Suitability of Eastern Gamagrass for In Situ Precipitation Catchment Forage Production in Playas SO AGRONOMY JOURNAL LA English DT Article ID ROOT PENETRATION; NITROGEN RATES; TRIPSACUM; SEED; GERMINATION; ESTABLISHMENT; DORMANCY; ALFALFA; TEXAS; ACID AB Freshwater playas, shallow ephemeral lakes whose basins fill aft er periods of prolonged or intense rainfall, occupy 3 to 5% of the land in the Llano Estacado and are considered marginal farmlands. The goals of this work were to determine if a native species, eastern gamagrass, could be grown on playa soils and to preliminarily evaluate the suitability of gamagrass [Tripsacum dactyloides (L.) L.] as a component of an in situ precipitation catchment forage production system. Flooding tolerance of gamagrass seeds was evaluated, and plants were grown on playa soil in both the greenhouse and field for several seasons. Gamagrass seed was resistant to extended flooding, especially at low ambient temperatures typically encountered in late fall or winter. Gamagrass roots penetrated nearly 2 m into waterlogged playa soil in greenhouse experiments. Stands were successfully established in playas, and plants survived for 6 yr when crowns were not destroyed by close mowing or vehicles. Eastern gamagrass is a viable candidate species that might be used as part of a novel playa-based forage production system. C1 [Gitz, Dennis C., III; Stout, John E.; Lascano, Robert J.; Velten, Jeff P.] USDA ARS, Lubbock, TX 79415 USA. [Baker, Jeffrey T.] USDA ARS, Cropping Syst Res Lab, Big Spring, TX 79720 USA. [Brauer, David K.] USDA ARS, Bushland, TX 79012 USA. RP Gitz, DC (reprint author), USDA ARS, 3810 4th St, Lubbock, TX 79415 USA. EM dennis.gitz@ars.usda.gov FU USDA-ARS, Kansas State University; Texas AgriLife Research; Texas AgriLife Extension Service; Texas Tech University; West Texas AM University FX We thank Dr. L. Liu-Gitz for helpful discussions and suggestions through the course of this experiment, Cody Byars for his technical assistance, Dr. Teddy Zobeck for his assistance locating and gaining access to suitable playas, and the landholders whose continued cooperation is essential for progress in this area. Comments by three anonymous reviewers were without exception constructive and considerably improved the quality of this report. This research was supported in part by the Ogallala Aquifer Program, a consortium between the USDA-ARS, Kansas State University, Texas AgriLife Research, Texas AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 39 TC 0 Z9 0 U1 1 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 907 EP 914 DI 10.2134/agronj2012.0358 PG 8 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100001 ER PT J AU Rao, SC Northup, BK AF Rao, Srinivas C. Northup, Brian K. TI Biomass Production and Quality of Indian-Origin Forage Guar in Southern Great Plains SO AGRONOMY JOURNAL LA English DT Article ID WARM-SEASON LEGUMES; CAPABILITIES; YIELD; CROP AB Guar [Cyamopsis tetragonoloba (L.) Taub.] is a summer annual pulse grown in semiarid regions of the world as a multi-purpose vegetable, grain, cover, or fodder crop. Existing guar cultivars used in the U. S. southern Great Plains (SGP) were developed for grain production, and there are currently no forage cultivars available for the region. This study compared biomass production and nutritive values of plant products of three cultivars of Indian origin, forage-type guar with a commonly used grain cultivar (Kinman) to determine whether the Indian-origin cultivars would be better production options for the SGP. Aboveground biomass was sampled at early and late vegetative stages, and physiological maturity. Samples collected at maturity were separated into grain, pods, and vegetative biomass. Nitrogen and in vitro digestible dry matter (IVDDM) concentrations were determined on all biomass components. Data were analyzed using mixed models with guar cultivars and growth stage as main effects, and years as a longitudinal (repeated) element. The Indian-origin cultivars produced significantly (P < 0.05) more forage (13-22%) and less grain (26-36%) than Kinman. Growing season and time of season main effects on biomass production, N, and IVDDM concentrations of forage, pods, and grain were significant. Drier growing seasons produced 50 and 34% less forage and grain, respectively, that contained 26 and 16% more N, with 14 and 6% greater IVDDM. Results indicated additional genetic selection is required to identify productive and reliable forage-type guar cultivars that are better suited to the variable growing conditions of the SGP. C1 [Rao, Srinivas C.; Northup, Brian K.] USDA ARS, Grazinglands Res Lab, El Reno, OK 73036 USA. RP Rao, SC (reprint author), USDA ARS, Grazinglands Res Lab, 7207 W Cheyenne St, El Reno, OK 73036 USA. EM srinivas.rao@ars.usda.gov NR 26 TC 1 Z9 1 U1 0 U2 2 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 945 EP 950 DI 10.2134/agronj2012.0378 PG 6 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100006 ER PT J AU Collins, HP Streubel, J Alva, A Porter, L Chaves, B AF Collins, Harold P. Streubel, Jason Alva, Ashok Porter, Lyndon Chaves, Bernardo TI Phosphorus Uptake by Potato from Biochar Amended with Anaerobic Digested Dairy Manure Effluent SO AGRONOMY JOURNAL LA English DT Article ID CATION-EXCHANGE CAPACITY; INDUCED CHEMICAL-CHANGES; SOLANUM-TUBEROSUM L.; WASTE-WATER; SOIL; PYROLYSIS; CHARCOAL; NITROGEN; YIELD; PLANT AB Sorption of plant nutrients from dairy storage lagoons by biochar and use as a supplemental fertilizer is a beneficial strategy to reduce nutrient contamination around dairies and supply nutrients to potato (Solanum tuberosum L.) and other crops. This research evaluated potato growth responses and P partitioning from several rates of P applied as either mono-ammonium phosphate (MAP) or as P recovered from anaerobic digested dairy manure using biochar. Single-stemmed plants of the potato cultivars Ranger Russet and Umatilla Russet were grown in 7-L pots containing Quincy sand soil (mixed, mesic Xeric Torripsamment). Biochar amended with dairy effluent applied at 5.7 and 11.4 Mg ha(-1) with fertilizer application rates equivalent to 55 and 110 kg P ha(-1) maintained adequate P levels in potato petioles of both cultivars through 85 d after planting. Total plant P uptake was greater for treatments receiving MAP. Total potato biomass and tuber yields of Umatilla Russet were 30 and 27% lower when amended with basic or enriched biochar compared to MAP treatments, respectively. Tuber biomass declined 10 and 20% for Ranger Russet and Umatilla Russet for the 11.4 Mg ha(-1) amended biochar, respectively compared to MAP fertilized treatments. Similar declines were found for the aboveground biomass and roots. The 5.7 Mg ha(-1) of enriched biochar supplied 70 to 80% of the P requirement for potato growth. These findings are an important step in providing evidence of the benefits of using recovered P as a fertilizer supplement to reduce the reliance on rock phosphates. C1 [Collins, Harold P.; Alva, Ashok; Porter, Lyndon; Chaves, Bernardo] USDA ARS, Prosser, WA 99350 USA. [Streubel, Jason] Convoy Hope, Childrens Feeding Initiat, Springfield, MO 65802 USA. RP Collins, HP (reprint author), USDA ARS, 24106 N, Bunn Rd, Prosser, WA 99350 USA. EM hal.collins@ars.usda.gov NR 69 TC 0 Z9 0 U1 7 U2 54 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 989 EP 998 DI 10.2134/agronj2012.0363 PG 10 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100011 ER PT J AU Ottman, MJ Hunt, LA White, JW AF Ottman, Michael J. Hunt, L. Anthony White, Jeffrey W. TI Photoperiod and Vernalization Effect on Anthesis Date in Winter-Sown Spring Wheat Regions SO AGRONOMY JOURNAL LA English DT Article ID MODELS; SIMULATION AB Accurate prediction of phenology is required to guide crop management decisions and to predict crop growth and yield. However, the relative importance of photoperiod and vernalization in predicting anthesis dates for spring bread and durum wheat (Triticum aestivum L. and T. durum Desf.) sown in the winter has not been reported. The purpose of this research is to determine the improvement in predicting anthesis dates of spring wheat sown in the winter when photoperiod and vernalization are considered. Observed dates of anthesis were obtained from University of Arizona wheat variety trials conducted at Maricopa, Wellton, and Yuma, AZ. The Cropping Systems Model CROPSIM-CERES as released in DSSAT 4.5 was used to simulate days to anthesis based on temperature, daylength, and vernalization. For 12 bread and durum wheat cultivars, the model predicted days to anthesis with a root mean square error (RMSE) of 7.6 d if all cultivar differences were ignored, 6.4 d considering only differences in thermal time (TT), 6.1 d with differences in TT and daylength response, 6.4 d with TT and vernalization, and 6.2 d with TT, daylength, and vernalization. Consideration of cultivar differences in TT and photoperiod response improved the prediction of days to anthesis for winter-sown spring wheat, but there was no benefit from considering effects of vernalization in CROPSIM-CERES. C1 [Ottman, Michael J.] Univ Arizona, CALS, Tucson, AZ 85721 USA. [Hunt, L. Anthony] Univ Guelph, Guelph, ON N1G 2W1, Canada. [White, Jeffrey W.] USDA ARS, ALARC, Maricopa, AZ 85138 USA. RP Ottman, MJ (reprint author), Univ Arizona, CALS, 1140 E South Campus Dr,Forbes 303,POB 210036, Tucson, AZ 85721 USA. EM mottman@ag.arizona.edu FU Agricultural Research Service, U.S. Department of Agriculture FX Western Plant Breeders conducted the trials at Wellton and Yuma, and we are grateful to Kim Shantz and his crew for their cooperation. The technical assistance of Mary Comeau and Mike Sheedy at Maricopa is greatly appreciated. This research was supported in part by the Agricultural Research Service, U.S. Department of Agriculture. The USDA and the University of Arizona are equal opportunity providers and employers. NR 19 TC 2 Z9 2 U1 0 U2 28 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 1017 EP 1025 DI 10.2134/agronj2012.0425 PG 9 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100014 ER PT J AU Baumhardt, RL Schwartz, R Howell, T Evett, SR Colaizzi, P AF Baumhardt, R. Louis Schwartz, Robert Howell, Terry Evett, Steven R. Colaizzi, Paul TI Residue Management Effects on Water Use and Yield of Deficit Irrigated Cotton SO AGRONOMY JOURNAL LA English DT Article ID TEXAS HIGH-PLAINS; GREAT-PLAINS; DRYLAND COTTON; WINTER-WHEAT; LINT YIELD; SOIL; TILLAGE; CROP; EVAPOTRANSPIRATION; INFILTRATION AB The declining saturated thickness of the Ogallala Aquifer beneath the southern High Plains decreases irrigation well capacity and necessitates conservation of precipitation for crop use. A 3-yr dryland crop rotation of wheat (Triticum aestivum L.) followed by cotton (Gossypium hirsutum L.) with intervening 10-mo fallow periods was adapted for use with deficit irrigation of 2.5 and 5.0 mm d(-1) capacities on a Pantex silty clay loam (fine, mixed, superactive, thermic Torrertic Paleustoll) managed with disk (DT), stubble-mulch (SM), or no (NT)-tillage at Bushland, TX (35.183 degrees N, 102.1 degrees W). Study objectives were to quantify tillage effects on soil water storage during wheat fallow and any continued effects on water use and yield by deficit irrigated cotton. Compared with DT, wheat residue cover increased mean fallow precipitation storage by similar to 15 mm for SM and 50 mm with NT based on significant differences in 3 of 4 yr. The significant differences in cotton water use between irrigation capacities totaled from 70% to similar to 100% of the estimated evapotranspiration, ET. Measured growing season water use decreased with decreasing residue cover in the order NT > SM > DT. Cotton lint yield did not vary between irrigation capacities, but increased with NT by similar to 50% or 450 kg ha(-1) over DT. Yields for DT cotton irrigated at 5 mm d(-1) were typically less than for NT and SM cotton irrigated at 2.5 mm d(-1). Lint yield for NT management was significantly greater than DT, which we attributed to reduced evaporation and greater transpiration with NT residue. C1 [Baumhardt, R. Louis; Schwartz, Robert; Howell, Terry; Evett, Steven R.; Colaizzi, Paul] USDA ARS, Bushland, TX 79012 USA. RP Baumhardt, RL (reprint author), USDA ARS, PO Drawer 10, Bushland, TX 79012 USA. EM r.louis.baumhardt@ars.usda.gov FU USDA Agricultural Research Service; Kansas State University; Texas A&M AgriLife Research; Texas A&M AgriLife Extension Service; Texas Tech University; West Texas AM University FX This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 36 TC 1 Z9 1 U1 1 U2 25 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 1026 EP 1034 DI 10.2134/agronj2012.0361 PG 9 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100015 ER PT J AU Baumhardt, RL Schwartz, R Howell, T Evett, SR Colaizzi, P AF Baumhardt, R. Louis Schwartz, Robert Howell, Terry Evett, Steven R. Colaizzi, Paul TI Residue Management Effects on Water Use and Yield of Deficit Irrigated Corn SO AGRONOMY JOURNAL LA English DT Article ID PLANT-DENSITY; WINTER-WHEAT; CROP RESIDUE; GREAT-PLAINS; STRAW-MULCH; SOIL; TILLAGE; EVAPORATION; DRYLAND; EVAPOTRANSPIRATION AB Residue management to increase fallow precipitation storage and reduce growing season evaporation limited potential yield reduction by off setting a portion of the decreasing irrigation well capacity of the declining Ogallala Aquifer in the southern High Plains. A 3-yr dryland rotation of wheat (Triticum aestivum L.) followed by corn (Zea mays L.) with intervening 10-mo fallow periods was adapted for use with deficit irrigation of 2.5 and 5.0 mm d(-1) capacities on a Pantex silty clay loam (fine, mixed, superactive, thermic Torrertic Paleustoll) at Bushland, TX (35 degrees 11' N, 102 degrees 6' W). Study objectives were to quantify disk (DT), stubble-mulch (SM), or no (NT)-tillage effects on precipitation storage during wheat fallow and subsequent use for grain yield by deficit irrigated corn. The 4-yr mean soil water after fallow increased from 14 to 50 mm for SM and NT compared with DT. Measured growing season water use ranged from similar to 54 to 64% of the estimated corn water use (ETc) at the 2.5 mm d(-1) irrigation capacity and from 76 to 85% at 5.0 mm d(-1) irrigation with corresponding grain yields ranging from crop failure to similar to 7.4 Mg ha(-1). We conclude that residue retaining conservation tillage increased soil water storage during fallow. We attributed greater corn yield with conservation tillage to likely increased transpiration made possible by reducing early growing season evaporation. Increased soil water and greater transpiration using conservation tillage practices did not off set corn ET reduction imposed by either deficit irrigation capacity and resulted in unprofitable crop yield reductions. C1 [Baumhardt, R. Louis; Schwartz, Robert; Howell, Terry; Evett, Steven R.; Colaizzi, Paul] ARS, USDA, Bushland, TX 79012 USA. RP Baumhardt, RL (reprint author), ARS, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM r.louis.baumhardt@ars.usda.gov FU USDA Agricultural Research Service; Kansas State University; Texas A&M AgriLife Research; Texas A&M AgriLife Extension Service; Texas Tech University; West Texas AM University FX This research was supported in part by the Ogallala Aquifer Program, a consortium between USDA Agricultural Research Service, Kansas State University, Texas A&M AgriLife Research, Texas A&M AgriLife Extension Service, Texas Tech University, and West Texas A&M University. NR 43 TC 5 Z9 5 U1 0 U2 22 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 EI 1435-0645 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 1035 EP 1044 DI 10.2134/agronj2012.0362 PG 10 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100016 ER PT J AU Mourtzinis, S Arriaga, FJ Balkcom, KS Ortiz, BV AF Mourtzinis, Spyridon Arriaga, Francisco J. Balkcom, Kipling S. Ortiz, Brenda V. TI Corn Grain and Stover Yield Prediction at R1 Growth Stage SO AGRONOMY JOURNAL LA English DT Article ID IN-SEASON PREDICTION; NITROGEN UPTAKE; PLANT HEIGHT; VARIABILITY AB Estimating corn (Zea mays L.) grain and stover yield during the growing season is an appealing idea. An accurate yield estimation could benefit farmers, as well as corn-related industries. The objective of this study was to develop regression equations to estimate corn grain and stover yield using easily accessible information (N fertilization rate and cumulative precipitation), and simple plant morphological measurements such as height, stem diameter at various heights, height of the first ear, and plants per hectare. Measurements made at silking (R1) were used since the maximum explainable variability would not exceed 58% at early stages of plant development (V2-V10; between 2-leaf and 10-leaf stages). The experiment was conducted from 2009 until 2011 in two locations, in north and central Alabama, under no-tillage and non-irrigated conditions. Treatments were assigned to a 3 x 4 x 2 complete factorial design arranged in a split-split-plot with three replications. Factors were; winter rye (Secale cereale L.) cover crop (main plot), N fertilization rates (subplot), and stover residue harvest (sub-subplot). All measurements from this study, across years and locations, were used in the final regression equations to create robust prediction models. Equations, with and without intercept, were developed and compared according to several statistical criteria. The final grain yield equation at R1 growth stage included an intercept with a R-2 of 0.7705. The final stover equation also included an intercept (R-2 = 0.8473). This study suggests that N rate, total precipitation amount from planting until silking, and simple plant morphological measurements can be used to predict corn yield. C1 [Mourtzinis, Spyridon] Auburn Univ, Auburn, AL 36832 USA. [Arriaga, Francisco J.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. [Balkcom, Kipling S.] ARS, USDA, Auburn, AL 36832 USA. [Ortiz, Brenda V.] Auburn Univ, Auburn, AL 36849 USA. RP Arriaga, FJ (reprint author), Univ Wisconsin, Dept Soil Sci, 1525 Observ Dr, Madison, WI 53706 USA. EM farriaga@wisc.edu NR 19 TC 8 Z9 8 U1 2 U2 19 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 1045 EP 1050 DI 10.2134/agronj2012.0393 PG 6 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100017 ER PT J AU Yost, MA Russelle, MP Coulter, JA AF Yost, Matt A. Russelle, Michael P. Coulter, Jeffrey A. TI Nitrogen Requirements of First-Year Corn following Alfalfa Were Not Altered by Fall-Applied Manure SO AGRONOMY JOURNAL LA English DT Article ID SOIL NITRATE; NO-TILLAGE; FERTILIZATION; AVAILABILITY; MANAGEMENT; TESTS; YIELD; RECOMMENDATIONS; CORNSTALKS; DAIRY AB Although farmers in the midwestern United States often apply livestock manure when terminating alfalfa (Medicago sativa L.), there are no published reports on direct effects of fall manure application on alfalfa N credits to first-year corn (Zea mays L.). Therefore, eight on-farm experiments were conducted in Minnesota to test whether manure applied during alfalfa termination in fall affects the rate of fertilizer N needed for the subsequent corn crop. Manure application rates were determined by cooperating growers and N fertilizer rates were 0, 45, 90 or 179 kg N ha(-1) at planting and 45 kg N ha(-1) as a sidedress. At five locations, neither manure nor fertilizer N increased grain or silage yield. At three remaining locations, N fertilizer was needed to economically optimize grain (98 kg N ha(-1) at two locations) or silage yield (113 kg N ha(-1) at three locations), but manure did not alter the response to fertilizer N. The presidedress soil nitrate test (PSNT) predicted the need for N fertilizer to optimize grain yield 63% of the time and the basal stalk nitrate test (BSNT) identified the need for N fertilizer 75% of the time. When results of this study were combined with data from the literature, the PSNT was accurate only 55% of the time and the BSNT could not separate responsive and nonresponsive sites. These results confirm that first-year corn following alfalfa oft en does not require supplemental N for maximum grain yield, and that more accurate methods are needed to predict fertilizer N response. C1 [Yost, Matt A.; Coulter, Jeffrey A.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Russelle, Michael P.] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. RP Yost, MA (reprint author), Univ Minnesota, Dept Agron & Plant Genet, 1991 Upper Buford Circle, St Paul, MN 55108 USA. EM yostx051@umn.edu FU North Central Region-Sustainable Agriculture Research and Education Program; Minnesota Agricultural Water Resources Center; Hueg-Harrison Fellowship FX This research was supported by the North Central Region-Sustainable Agriculture Research and Education Program, the Minnesota Agricultural Water Resources Center, and the Hueg-Harrison Fellowship. The authors appreciate the help from numerous extension educators, state agency personnel, and private consultants to identify potential cooperators, and the technical help from Ryan Maher, Eric Ristau, Diogo Silva, Jean De Santos, Robert Renier, Daniel Silveira, Tamas Varga, Isaias de Mambro, and eight growers. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 44 TC 3 Z9 3 U1 3 U2 12 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 1061 EP 1069 DI 10.2134/agronj2012.0496 PG 9 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100019 ER PT J AU Riedell, WE Osborne, SL Pikul, JL AF Riedell, Walter E. Osborne, Shannon Lynn Pikul, Joseph L., Jr. TI Soil Attributes, Soybean Mineral Nutrition, and Yield in Diverse Crop Rotations under No-Till Conditions SO AGRONOMY JOURNAL LA English DT Article ID ELECTRICAL-CONDUCTIVITY; NUTRIENT-UPTAKE; BULK-DENSITY; ROOT-GROWTH; NITROGEN; CORN; MANAGEMENT; ALFALFA; NITRATE; AGRICULTURE AB Development of sustainable crop rotations depends on understanding complex relationships among soils, crops, and yield. Our objectives were to measure how soil chemical and physical attributes as well as soybean [Glycine max (L.) Merr.] stover dry weight and mineral concentrations, seed yield, and seed composition (protein, oil, and minerals) responded to a soybean-maize (Zea mays L.) 2-yr rotation (S-C), soybean-spring wheat (Triticum aestivum L.)maize 3-yr rotation (S-W-C), soybean-oat/pea (Hordeum vulgare L./Pisum sativum L.) hay-maize 3-yr rotation (S-H-C), and soybean-oat/pea hay underseeded with alfalfa (Medicago sativa L.)-alfalfa-alfalfa-maize 5-yr rotation (S-H/A-A-A-C). Rotation treatments under no-till soil management were established in 1997 and soybean measurements were made during the 2009 and 2010 growing seasons. Soils under the 5-yr rotation had 129 kg ha(-1) preseason residual NO3-N concentration, while the other rotations averaged about 65 kg ha(-1). The 5-yr rotation also had lower bulk density (1.35 g cm(-3)) than S-H-C (1.46 g cm(-3)), with S-W-C (1.38 g cm(-3)) and S-C (1.38 g cm(-3)) intermediate. Soybean seed yield was about 10% greater in the 5-yr rotation than in the other treatments. Kernel protein was 3% greater and kernel Zn was 11% greater under the 5-yr rotation than under the S-C rotation, while the others were intermediate. Changes in soil physical and chemical properties under extended rotations that include perennial forages may play an important role in increasing soybean seed yield and enhancing seed protein but may, because of high preseason soil NO3-N levels, also be susceptible to potential NO3-N leaching beyond the root zone. C1 [Riedell, Walter E.; Osborne, Shannon Lynn; Pikul, Joseph L., Jr.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Riedell, WE (reprint author), ARS, USDA, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM walter.riedell@ars.usda.gov NR 47 TC 2 Z9 2 U1 3 U2 32 PU AMER SOC AGRONOMY PI MADISON PA 677 S SEGOE RD, MADISON, WI 53711 USA SN 0002-1962 J9 AGRON J JI Agron. J. PD JUL-AUG PY 2013 VL 105 IS 4 BP 1231 EP 1236 DI 10.2134/agronj2013.0037 PG 6 WC Agronomy SC Agriculture GA 170MH UT WOS:000320855100037 ER PT J AU Brown, GG Callaham, MA Niva, CC Feijoo, A Sautter, KD James, SW Fragoso, C Pasini, A Schmelz, RM AF Brown, George G. Callaham, Mac. A., Jr. Niva, Cintia C. Feijoo, Alexander Sautter, Klaus D. James, Samuel W. Fragoso, Carlos Pasini, Amarildo Schmelz, Ruediger M. TI Terrestrial oligochaete research in Latin America: The importance of the Latin American Meetings on Oligochaete Ecology and Taxonomy SO APPLIED SOIL ECOLOGY LA English DT Article DE Enchytraeids; Earthworms; Biodiversity; Capacity building; Taxonomic impediment ID PONTOSCOLEX-CORETHRURUS GLOSSOSCOLECIDAE; ATLANTICA ENCHYTRAEIDS PARANA; ORGANIC-MATTER DYNAMICS; LAND-USE SYSTEMS; EARTHWORM POPULATIONS; SOIL MACROFAUNA; AGRICULTURAL INTENSIFICATION; ADAPTIVE STRATEGIES; EISENIA-FOETIDA; TROPICAL SOIL AB Over 1100 species of oligochaetes are known from Latin America (LA), of which most are earthworms. Enchytraeid research is not so well developed, mainly due to their small size, the difficulty of identification, and the general ignorance of LA scientists of this family. Some countries, such as Brazil, Colombia and Mexico have active taxonomists, and hence tend to have better knowledge of oligochaete diversity and ecology. However, the few professionals active in the area and a major taxonomic impediment have greatly hindered research on oligochaetes in LA. Four Latin American Meetings on Oligochaete Ecology and Taxonomy (ELAETAO) have been held since 2003. These meetings brought together researchers, students and technicians from 15 countries and resulted in several landmark publications (3 special journal issues and one book) that synthesize research performed in the Americas. Topics addressed include the diversity and distribution of native and exotic earthworm species in all LA countries, the use of oligochaetes as bioindicators, oligochaete genetics and phylogeny, various aspects of vermiculture, earthworm and enchytraeid sampling methods, and ecology and biology of invasive species, among others. Furthermore, since the first ELAETAO, regular training in terrestrial oligochaete ecology and taxonomy (10 earthworm and 2 enchytraeid short courses), as well as in ecotoxicology and molecular genetics has been offered to 150 scientists and students in these topics in order to build capacity for research activity. The ELAETAOs have greatly stimulated earthworm and enchytraeid research in LA, but several challenges must still be overcome to adequately assess the true nature of oligochaete biodiversity and their ecology in the region. (C) 2013 Elsevier B.V. All rights reserved. C1 [Brown, George G.; Niva, Cintia C.] Embrapa Florestas, BR-83411000 Colombo, PR, Brazil. [Callaham, Mac. A., Jr.] USDA, Ctr Forest Disturbance Sci, Forest Serv, Athens, GA 30602 USA. [Feijoo, Alexander] Univ Tecnol Pereira, Fac Ciencias Ambientales, Pereira 097, Colombia. [Sautter, Klaus D.] Univ Posit, BR-81280330 Curitiba, Parana, Brazil. [James, Samuel W.] Univ Iowa, Dept Biol, Iowa City, IA 52242 USA. [Fragoso, Carlos] Inst Ecol AC, Xalapa 91070, Veracruz, Mexico. [Pasini, Amarildo] Univ Estadual Londrina, Dept Agron, BR-86051990 Londrina, PR, Brazil. [Schmelz, Ruediger M.] Univ A Coruna, Fac Sci, Dept Anim Biol Plant Biol & Ecol, E-15008 La Coruna, Spain. RP Brown, GG (reprint author), Embrapa Florestas, Estr Ribeira Km 111,CP 319, BR-83411000 Colombo, PR, Brazil. EM george.brown@embrapa.br; mcallaham@fs.fed.us; cintiacn@gmail.com; afeijoo@utp.edu.co; ksautter@up.edu.br; samuel-james@uiowa.edu; carlos.fragoso@inecol.edu.mx; pasini@uel.br; rmschmelz@gmail.com RI Niva, Cintia/C-1143-2014; Brown, George/D-4616-2012; OI Niva, Cintia/0000-0001-9306-4553; Brown, George/0000-0001-9550-6909; Fragoso, Carlos/0000-0001-7076-6712 FU Embrapa Florestas; CAPES; CNPq; Fundacao Araucaria; Fundacao Agrisus; CONACYT-Mexico; USA National Science Foundation; Universidad de A Coruna; Fulbright Foundation; USDA; Universidad Tecnologica de Pereira FX The 4th ELAETAO received financial support from Embrapa Florestas, CAPES, CNPq, Fundacao Araucaria and Fundacao Agrisus. Logistical support was provided by Embrapa Florestas, UFPR, UEL and Universidade Positivo. The authors also thank P. Lavelle, C. Csuzdi and J. Rombke for their helpful comments and the support of CNPq (fellowships to G.G. Brown & C.C. Niva), CONACYT-Mexico (C. Fragoso), the USA National Science Foundation (S.W. James), Universidad de A Coruna (R.M. Schmelz), Fulbright Foundation and USDA (M.A. Callaham Jr.) and Universidad Tecnologica de Pereira (A. Feijoo). NR 191 TC 2 Z9 2 U1 3 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD JUL PY 2013 VL 69 SI SI BP 2 EP 12 DI 10.1016/j.apsoil.2012.12.006 PG 11 WC Soil Science SC Agriculture GA 165OT UT WOS:000320493900002 ER PT J AU Breitenbach, JE Popham, HJR AF Breitenbach, Jonathan E. Popham, Holly J. R. TI Baculovirus replication induces the expression of heat shock proteins in vivo and in vitro SO ARCHIVES OF VIROLOGY LA English DT Article ID INSECT CELLS; SACCHAROMYCES-CEREVISIAE; GENE-EXPRESSION; HOST-RANGE; EVOLUTION; HSP70; LINES; CHAPERONES; PESTICIDES; INHIBITORS AB A recent handful of studies have linked baculovirus infection with the induction of heat shock proteins, a highly conserved family of cytoprotective proteins. Here, we demonstrate baculovirus-stimulated upregulation of hsp70 transcription in the natural host, Helicoverpa zea. Larvae lethally infected with Helicoverpa zea single nucleopolyhedrovirus (HzSNPV) accumulated hsp70 transcripts throughout the 72-hour course of infection in the midgut, hemocytes, and fat body. While a maximal 17- or 15-fold induction of hsp70 was noted in the midgut and hemocytes, respectively, by 72 hours postinfection, the level of hsp70 transcription in the fat body of larvae was greater than two orders of magnitude higher than in mock-infected larvae. These results were largely mirrored in cultures of infected cells, and a potentiation effect was observed in cells that were both heat shocked and infected. In contrast, Spodoptera frugiperda multiple nucleopolyhedrovirus and ultraviolet-inactivated HzSNPV did not stimulate hsp70 transcription in these non-permissive larvae and in cell culture, respectively. Taken together, this report documents baculovirus-mediated upregulation of hsp70 in the host and demonstrates the requirement for productive infection for hsp70 induction in vitro and in vivo. C1 [Breitenbach, Jonathan E.; Popham, Holly J. R.] Agr Res Serv, Biol Control Insects Res Lab, USDA, Columbia, MO 65203 USA. RP Popham, HJR (reprint author), Agr Res Serv, Biol Control Insects Res Lab, USDA, 1503 S Providence Rd, Columbia, MO 65203 USA. EM pophamh@missouri.edu NR 46 TC 5 Z9 5 U1 1 U2 22 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 J9 ARCH VIROL JI Arch. Virol. PD JUL PY 2013 VL 158 IS 7 BP 1517 EP 1522 DI 10.1007/s00705-013-1640-8 PG 6 WC Virology SC Virology GA 173GN UT WOS:000321066600008 PM 23443933 ER PT J AU Wiegner, TN Hughes, F Shizuma, LM Bishaw, DK Manuel, ME AF Wiegner, Tracy N. Hughes, Flint Shizuma, Lisa M. Bishaw, David K. Manuel, Mark E. TI Impacts of an Invasive N-2-Fixing Tree on Hawaiian Stream Water Quality SO BIOTROPICA LA English DT Article DE Falcataria moluccana; Hawaii; nitrogen; nutrient limitation; riparian zone; streams ID DISSOLVED ORGANIC-CARBON; ALDER ALNUS-RUBRA; RED ALDER; FALCATARIA-MOLUCCANA; EUCALYPTUS-SALIGNA; NUTRIENT LIMITATION; ALBIZIA-FACALTARIA; NITROGEN EXPORT; MOUNTAIN STREAM; FOREST SOILS AB N-2-fixing trees can affect stream water quality. This has been documented in temperate streams, but not in tropical ones, even though N-2-fixing trees are prevalent in the tropics. We investigated the effects of the introduced, invasive tree, Falcataria moluccana (albizia) on water quality of Hawaiian streams. Nutrient concentrations were measured in reaches above and below F.moluccana-dominated riparian zones of four streams over 14mo, and benthic algal nutrient limitation was examined in one stream. NO3-+NO2- concentrations were up to 600 percent higher in reaches below F.moluccana stands than in ones above them. In contrast, dissolved organic nitrogen concentrations were 24 percent lower in most reaches below F.moluccana stands, and NH4+ and particulate nitrogen concentrations were similar above and below the stands. Dissolved organic carbon concentrations were up to 30 percent lower below F.moluccana stands, but particulate carbon concentrations were similar between reaches. Total dissolved phosphorus concentrations were similar above and below F.moluccana stands, whereas H4SiO4 concentrations were higher below the stands. In the stream where benthic chlorophyll a was measured, concentrations were three times higher below the F.moluccana stand than above it. Benthic algae were co-limited by nitrogen and phosphorus above the F.moluccana stand, and limited by phosphorus below it. These results suggest that F.moluccana's presence relieved nitrogen-limitation and caused the benthic algae to become solely phosphorus-limited. Overall, our results demonstrate that F.moluccana can strongly affect the chemistry and primary producers of these tropical streams. C1 [Wiegner, Tracy N.; Manuel, Mark E.] Univ Hawaii, Dept Marine Sci, Hilo, HI 96720 USA. [Hughes, Flint] Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, Hilo, HI 96720 USA. [Shizuma, Lisa M.] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. [Bishaw, David K.] Univ Hawaii, Coll Agr, Hilo, HI 96720 USA. RP Wiegner, TN (reprint author), Univ Hawaii, Dept Marine Sci, 200 West Kawili St, Hilo, HI 96720 USA. EM wiegner@hawaii.edu FU National Science Foundation (NSF) [DBI04-53630]; National Ocean and Atmospheric Administration (NOAA, Minority Serving Institution Environmental Educational Partnership grant); Keaholoa program at UHH (NSF Award) [0237065] FX We thank Mike Gomes of Surety Kohala Corporation for access to streams in the Kohala watershed, Randi Schneider of the University of Hawaii at Hilo's (UHH) Analytical Laboratory for sample analysis, Jim Baldwin for statistical analyses, Jene Michaud, Julie Gaertner, and Lisa Canale for geospatial analyses, Rich MacKenzie for nutrient difusing plates, and Sharon Ziegler-Chong, Drew Kapp, and Kamaka Gunderson for finacial support and guidance of undegraduate research assistants through the UHH Pacific Islands Program for Exploring Sciences (PIPES), which was supported by the National Science Foundation (NSF, Award #: DBI04-53630) and the National Ocean and Atmospheric Administration (NOAA, Minority Serving Institution Environmental Educational Partnership grant), and also through the Keaholoa program at UHH (NSF Award #: 0237065). NR 73 TC 6 Z9 6 U1 3 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0006-3606 J9 BIOTROPICA JI Biotropica PD JUL PY 2013 VL 45 IS 4 BP 409 EP 418 DI 10.1111/btp.12024 PG 10 WC Ecology SC Environmental Sciences & Ecology GA 175DQ UT WOS:000321210900001 ER PT J AU Grez, AA Rand, TA Zaviezo, T Castillo-Serey, F AF Grez, Audrey A. Rand, Tatyana A. Zaviezo, Tania Castillo-Serey, Francisca TI Land use intensification differentially benefits alien over native predators in agricultural landscape mosaics SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE Biodiversity; Coccinellidae; habitat specialization; ladybeetles; specialists and generalists ID HARMONIA-AXYRIDIS; COCCINELLIDS COLEOPTERA; UNITED-STATES; ABUNDANCE; LADYBIRDS; INVASION; DECLINE; FRAGMENTATION; CONSEQUENCES; BIODIVERSITY AB Aim Both anthropogenic habitat disturbance and the breadth of habitat use by alien species have been found to facilitate invasion into novel environments, and these factors have been hypothesized to be important within coccinellid communities specifically. In this study, we address two questions: (1) Do alien species benefit more than native species from human-disturbed habitats? (2) Are alien species more generalized in their habitat use than natives within the invaded range or can their abundance patterns be explained by specialization on the most common habitats? Location Chile. Methods We quantified the use of different habitat types by native and alien coccinellid beetles by sampling individuals in nine habitat types that spanned a gradient in disturbance intensity, and represented the dominant natural, semi-natural and agricultural habitats in the landscape. Results Our results provide strong support for the hypotheses that more-disturbed habitats are differentially invaded. Both the proportion of alien individuals and the proportion of alien species increased significantly with increasing disturbance intensity. In contrast, we found no evidence that alien species were more generalized in their habitat use than native species; in fact, the trend was in the opposite direction. The abundance of specialized alien coccinellid species was not correlated with the area of the habitat types in the landscape. Main conclusion The results suggest that successfully established alien coccinellid species may be disturbance specialists' that thrive within human-modified habitats. Therefore, less-disturbed agroecosystems are desirable to promote the regional conservation of native species within increasingly human-dominated landscapes. C1 [Grez, Audrey A.; Castillo-Serey, Francisca] Univ Chile, Fac Ciencias Vet & Pecuarias, Santiago, Chile. [Rand, Tatyana A.] ARS, USDA, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. [Zaviezo, Tania] Pontificia Univ Catolica Chile, Fac Agron & Ing Forestal, Santiago, Chile. RP Grez, AA (reprint author), Univ Chile, Fac Ciencias Vet & Pecuarias, Casilla 2 Correo 15, Santiago, Chile. EM agrez@uchile.cl RI Grez, Audrey/G-8677-2013; Zaviezo, Tania/F-3099-2014 OI Grez, Audrey/0000-0002-6907-1283; FU FONDECYT [1100159] FX We thank FONDECYT 1100159 for supporting this study. We are grateful to Elizabeth Gazzano and students who helped with field work, Raphael Didham from CSIRO, Erin Espeland from USDA and three anonymous reviewers who made helpful comments to an early version of this article. NR 49 TC 8 Z9 10 U1 2 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 J9 DIVERS DISTRIB JI Divers. Distrib. PD JUL PY 2013 VL 19 IS 7 BP 749 EP 759 DI 10.1111/ddi.12027 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 163HU UT WOS:000320327500009 ER PT J AU Prasantha, BDR Amunogoda, PNRJ AF Prasantha, B. D. Rohitha Amunogoda, P. N. R. J. TI Moisture Adsorption Characteristics of Solar-Dehydrated Mango and Jackfruit SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Solar dehydrated; Jackfruit; Mango; Monolayer moisture; GAB; Isosteric heat ID WATER SORPTION ISOTHERMS; ARTOCARPUS-HETEROPHYLLUS; ISOSTERIC HEAT; GAB MODEL; TEMPERATURE; DESORPTION; PINEAPPLE; BEHAVIOR; RAISINS; RICE AB The moisture adsorption isotherms of solar dehydrated mango and jackfruit were determined at temperatures ranging from 30 A degrees C to 50 A degrees C. The equilibrium moisture content (EMC) of mango and jackfruit increased sharply as the temperature increased at water activity (a (w)) above 0.6 and 0.8, respectively. However, there were no clear isothermal intersection points observed at higher a (w) and temperatures. The EMC of solar dehydrated jackfruit showed the isothermal characteristics between types II and III. In contrast, dehydrated mango followed the characteristic type III adsorption isotherms due to high total soluble solids content of 67.8 A degrees Brix and total sugars of 14.21 g/100 g fresh mango. Estimated parameters and fitting ability of three isotherm models were also evaluated. The Guggenheim-Anderson-Boer (GAB) model gave the best fit to the experimental EMC data. The GAB monolayer moisture contents (m (o)) of mango and jackfruit ranged from 11.1-10.0 % and 4.7-3.4 %, respectively. Specific surface area of active binding sites (S) was calculated based on the m (o) values. The S value of dehydrated mango was 2.5 to 2.8 times larger than jackfruit. The maximum net isosteric heat (q (s)) of sorption of solar dehydrated mango and jackfruit were determined as 19.5 and 33 kJ mol(-1), respectively, and q (s) decreased significantly at high moisture. C1 [Prasantha, B. D. Rohitha] Univ Peradeniya, Fac Agr, Dept Food Sci & Technol, Peradeniya, Sri Lanka. [Amunogoda, P. N. R. J.] Ind Technol Inst, Colombo 07, Sri Lanka. RP Prasantha, BDR (reprint author), USDA ARS, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM rop_bd@yahoo.com NR 41 TC 3 Z9 3 U1 5 U2 14 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD JUL PY 2013 VL 6 IS 7 BP 1720 EP 1728 DI 10.1007/s11947-012-0832-7 PG 9 WC Food Science & Technology SC Food Science & Technology GA 168HF UT WOS:000320695700010 ER PT J AU Godinho, VM Furbino, LE Santiago, IF Pellizzari, FM Yokoya, NS Pupo, D Alves, TMA Junior, PAS Romanha, AJ Zani, CL Cantrell, CL Rosa, CA Rosa, LH AF Godinho, Valeria M. Furbino, Laura E. Santiago, Iara F. Pellizzari, Franciane M. Yokoya, Nair S. Pupo, Dicla Alves, Tania M. A. Junior, Policarpo A. S. Romanha, Alvaro J. Zani, Carlos L. Cantrell, Charles L. Rosa, Carlos A. Rosa, Luiz H. TI Diversity and bioprospecting of fungal communities associated with endemic and cold-adapted macroalgae in Antarctica SO ISME JOURNAL LA English DT Article DE Antarctica; marine fungi; seaweeds; diversity; extremophiles ID CYTOTOXIC METABOLITES; MARINE ALGA; PENICILLIUM; STRAINS; IDENTIFICATION; PENOSTATINS; TEMPERATURE; ISLANDS; REGION; DESV. AB We surveyed the distribution and diversity of fungi associated with eight macroalgae from Antarctica and their capability to produce bioactive compounds. The collections yielded 148 fungal isolates, which were identified using molecular methods as belonging to 21 genera and 50 taxa. The most frequent taxa were Geomyces species (sp.), Penicillium sp. and Metschnikowia australis. Seven fungal isolates associated with the endemic Antarctic macroalgae Monostroma hariotii (Chlorophyte) displayed high internal transcribed spacer sequences similarities with the psychrophilic pathogenic fungus Geomyces destructans. Thirty-three fungal singletons (66%) were identified, representing rare components of the fungal communities. The fungal communities displayed high diversity, richness and dominance indices; however, rarefaction curves indicated that not all of the fungal diversity present was recovered. Penicillium sp. UFMGCB 6034 and Penicillium sp. UFMGCB 6120, recovered from the endemic species Palmaria decipiens (Rhodophyte) and M. hariotii, respectively, yielded extracts with high and selective antifungal and/or trypanocidal activities, in which a preliminary spectral analysis using proton nuclear magnetic resonance spectroscopy indicated the presence of highly functionalised aromatic compounds. These results suggest that the endemic and cold-adapted macroalgae of Antarctica shelter a rich, diversity and complex fungal communities consisting of a few dominant indigenous or mesophilic cold-adapted species, and a large number of rare and/or endemic taxa, which may provide an interesting model of algal-fungal interactions under extreme conditions as well as a potential source of bioactive compounds. C1 [Godinho, Valeria M.; Furbino, Laura E.; Santiago, Iara F.; Rosa, Carlos A.; Rosa, Luiz H.] Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil. [Pellizzari, Franciane M.] Univ Estadual Parana, Lab Ficol & Qual Agua Mar, Paranagua, Brazil. [Yokoya, Nair S.; Pupo, Dicla] Nucleo Pesquisa Ficol, Secao Ficol, Sao Paulo, Brazil. [Alves, Tania M. A.; Zani, Carlos L.] Fundacao Oswaldo Cruz, Ctr Pesquisas Rene Rachou, Lab Quim Prod Nat, Belo Horizonte, MG, Brazil. [Junior, Policarpo A. S.; Romanha, Alvaro J.] Fiocruz MS, Ctr Pesquisas Rene Rachou, Lab Parasitol Celular & Mol, BR-30190 Belo Horizonte, MG, Brazil. [Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res Unit, Oxford, MS USA. RP Rosa, LH (reprint author), Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, Lab Sistemat & Biomol Fungos, POB 486, BR-31270901 Belo Horizonte, MG, Brazil. EM lhrosa@icb.ufmg.br RI Alves, Tania Maria/B-4814-2009; Yokoya, Nair/F-1571-2015; Zani, Carlos/A-9658-2008 OI Zani, Carlos/0000-0003-1859-177X FU Brazilian Antarctic Program (PROANTAR/MCT/CNPq) [23/2009]; Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG); Health-PDTIS-FIOCRUZ; PRPq-UFMG; CAPES/PGCI [036/13]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); Marine of Brazil; Clube Alpino Paulista (CAP) FX This study had financial and logistic support from the Brazilian Antarctic Program (PROANTAR/MCT/CNPq-No. 23/2009), Marine of Brazil and Francisco Petrone from the Clube Alpino Paulista (CAP). We acknowledge the financial support from Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), Health-PDTIS-FIOCRUZ, PRPq-UFMG, CAPES/PGCI 036/13 and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). This study represents the Pos-doctoral degree of Dr VM Godinho under the supervision of Dr LH Rosa. Also, the results of this study are part of the Master Science degree of LE Furbino within the Programa of Pos-Graduacao in Biotecnologia of UFOP. We thank Isis V Galliza and Fernanda LM Francisco for performing the biological assays. We thank Solomon Green III for technical assistance. We also thank the anonymous reviewers for helpful comments to improve the quality of the manuscript. NR 62 TC 21 Z9 22 U1 1 U2 48 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 J9 ISME J JI ISME J. PD JUL PY 2013 VL 7 IS 7 BP 1434 EP 1451 DI 10.1038/ismej.2013.77 PG 18 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA 170LM UT WOS:000320852100018 PM 23702515 ER PT J AU Mellon, JE Dowd, MK Beltz, SB AF Mellon, J. E. Dowd, M. K. Beltz, S. B. TI Effects of temperature and medium composition on inhibitory activities of gossypol-related compounds against aflatoxigenic fungi SO JOURNAL OF APPLIED MICROBIOLOGY LA English DT Article DE aflatoxin biosynthesis inhibition; Aspergillus flavus; Aspergillus parasiticus; natural products; terpenoids ID ASPERGILLUS-FLAVUS; CANCER CELLS; PROTEINS; APOGOSSYPOLONE; CYTOTOXICITY; GROWTH AB Aims To investigate the effects of temperature and medium composition on growth/aflatoxin inhibitory activities of terpenoids gossypol, gossypolone and apogossypolone against Aspergillus flavus and A.parasiticus. Methods and Results The compounds were tested at a concentration of 100gml-1 in a Czapek Dox (Czapek) agar medium at 25, 31 and 37 degrees C. Increased incubation temperature marginally increased growth inhibition caused by these compounds, but reduced the aflatoxin inhibition effected by gossypol. Gossypolone and apogossypolone retained good aflatoxin inhibitory activity against A.flavus and A.parasiticus at higher incubation temperatures. However, increased temperature also significantly reduced aflatoxin production in control cultures. The effects of the terpenoids on fungal growth and aflatoxin production against the same fungi were also determined in Czapek, Czapek with a protein/amino acid addendum and yeast extract sucrose (YES) media. Growth of these fungi in the protein-supplemented Czapek medium or in the YES medium greatly reduced the growth inhibition effects of the terpenoids. Apogossypolone displayed strong anti-aflatoxigenic activity in the Czapek medium, but this activity was significantly reduced in the protein-amended Czapek and YES media. Gossypol, which displayed little to no aflatoxin inhibitory activity in the Czapek medium, did yield significant anti-aflatoxigenic activity in the YES medium. Conclusions Incubation temperature and media composition are important parameters involved in the regulation of aflatoxin production in A.flavus and A.parasiticus. These parameters also affect the potency of growth and aflatoxin inhibitory activities of these gossypol-related compounds against aflatoxigenic fungi. Significance and Impact of the Study Studies utilizing gossypol-related compounds as inhibitory agents of biological activities should be interpreted with caution due to compound interaction with multiple components of the test system, especially serum proteins. C1 [Mellon, J. E.; Dowd, M. K.; Beltz, S. B.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Mellon, JE (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Jay.Mellon@ars.usda.gov NR 30 TC 3 Z9 3 U1 0 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1364-5072 J9 J APPL MICROBIOL JI J. Appl. Microbiol. PD JUL PY 2013 VL 115 IS 1 BP 179 EP 186 DI 10.1111/jam.12219 PG 8 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 162PX UT WOS:000320278600020 PM 23594138 ER PT J AU Bradner, L Robbe-Austerman, S Beitz, DC Stabel, JR AF Bradner, L. Robbe-Austerman, S. Beitz, D. C. Stabel, J. R. TI Chemical Decontamination with N-Acetyl-L-Cysteine-Sodium Hydroxide Improves Recovery of Viable Mycobacterium avium subsp paratuberculosis Organisms from Cultured Milk SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID TIME QUANTITATIVE PCR; CLINICAL SPECIMENS; DAIRY-CATTLE; SYSTEM; SPUTUM; PROTOCOLS; SAMPLES; SHEEP; BOVIS; NAOH AB Mycobacterium avium subsp. paratuberculosis is shed into the milk and feces of cows with advanced Johne's disease, allowing the transmission of M. avium subsp. paratuberculosis between animals. The objective of this study was to formulate an optimized protocol for the isolation of M. avium subsp. paratuberculosis in milk. The parameters investigated included chemical decontamination with N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH), alone and in combination with antibiotics (vancomycin, amphotericin B, and nalidixic acid), and the efficacy of solid (Herrold's egg yolk medium [HEY]) and liquid (Bactec 12B and para-JEM) culture media. For each experiment, raw milk samples from a known noninfected cow were inoculated with 10(2) to 10(8) CFU/ml of live M. avium subsp. paratuberculosis organisms. The results indicate that an increased length of exposure to NALC-NaOH from 5 to 30 min and an increased concentration of NaOH from 0.5 to 2.0% did not affect the viability of M. avium subsp. paratuberculosis. Additional treatment of milk samples with the antibiotics following NALC-NaOH treatment decreased the recovery of viable M. avium subsp. paratuberculosis cells more than treatment with NALC-NaOH alone. The Bactec 12B medium was the superior medium of the three evaluated for the isolation of M. avium subsp. paratuberculosis from milk, as it achieved the lowest threshold of detection. The optimal conditions for NALC-NaOH decontamination were determined to be exposure to 1.50% NaOH for 15 min followed by culture in Bactec 12B medium. This study demonstrates that chemical decontamination with NALC-NaOH resulted in a greater recovery of viable M. avium subsp. paratuberculosis cells from milk than from samples treated with hexadecylpyridinium chloride (HPC). Therefore, it is important to optimize milk decontamination protocols to ensure that low concentrations of M. avium subsp. paratuberculosis can be detected. C1 [Bradner, L.; Beitz, D. C.] Iowa State Univ, Roy J Carver Dept Biochem Biophys & Mol Biol, Ames, IA USA. [Robbe-Austerman, S.] USDA, APHIS, Vet Serv, Ames, IA USA. [Beitz, D. C.; Stabel, J. R.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Stabel, J. R.] USDA ARS, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Stabel, JR (reprint author), Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. EM judy.stabel@ars.usda.gov NR 30 TC 2 Z9 2 U1 0 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUL PY 2013 VL 51 IS 7 BP 2139 EP 2146 DI 10.1128/JCM.00508-13 PG 8 WC Microbiology SC Microbiology GA 166YB UT WOS:000320595800019 PM 23637290 ER PT J AU Leski, TA Bangura, U Jimmy, DH Ansumana, R Lizewski, SE Li, RW Stenger, DA Taitt, CR Vora, GJ AF Leski, Tomasz A. Bangura, Umaru Jimmy, David H. Ansumana, Rashid Lizewski, Stephen E. Li, Robert W. Stenger, David A. Taitt, Chris R. Vora, Gary J. TI Identification of bla(OXA-51-like), bla(OXA-58), bla(DIM-1), and bla(VIM) Carbapenemase Genes in Hospital Enterobacteriaceae Isolates from Sierra Leone SO JOURNAL OF CLINICAL MICROBIOLOGY LA English DT Article ID METALLO-BETA-LACTAMASE; ACINETOBACTER-BAUMANNII; MULTIPLEX PCR; RESISTANCE; SEQUENCES; EMERGENCE; OUTBREAK; ISABA1; RPOB AB We describe the results of a molecular epidemiological survey of 15 carbapenemase-encoding genes from a recent collection of clinical isolates from Mercy Hospital in Bo, Sierra Leone. The most salient findings revealed that (i) 60% of the isolates harbored multiple carbapenemase genes; (ii) the bla(DIM-1) gene, which has previously only been reported in The Netherlands, is also circulating in this environment; and (iii) bla(OXA-51-like) and bla(OXA-58) genes, which were thought to reside exclusively in Acinetobacter species, can also be found in members of the Enterobacteriaceae. C1 [Leski, Tomasz A.; Lizewski, Stephen E.; Stenger, David A.; Taitt, Chris R.; Vora, Gary J.] Naval Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA. [Bangura, Umaru; Jimmy, David H.; Ansumana, Rashid] Mercy Hosp, Res Lab, Kulanda Town, Bo, Sierra Leone. [Jimmy, David H.] Njala Univ, Dept Chem, Njala, Sierra Leone. [Ansumana, Rashid] Njala Univ, Inst Environm Management & Qual Control, Njala, Sierra Leone. [Ansumana, Rashid] Univ Liverpool, Liverpool Sch Trop Med, Liverpool L3 5QA, Merseyside, England. [Li, Robert W.] USDA, Anim & Nat Resources Inst, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. RP Vora, GJ (reprint author), Naval Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA. EM gary.vora@nrl.navy.mil RI Leski, Tomasz/K-6916-2013 OI Leski, Tomasz/0000-0001-7688-9887 FU Joint Science and Technology Office, Defense Threat Reduction Agency; Office of Naval Research via U.S. Naval Research Laboratory FX This work was supported in part by the Joint Science and Technology Office, Defense Threat Reduction Agency (D. A. S.), and by the Office of Naval Research via U.S. Naval Research Laboratory core funds (G.J.V.). NR 25 TC 11 Z9 11 U1 0 U2 5 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0095-1137 J9 J CLIN MICROBIOL JI J. Clin. Microbiol. PD JUL PY 2013 VL 51 IS 7 BP 2435 EP 2438 DI 10.1128/JCM.00832-13 PG 4 WC Microbiology SC Microbiology GA 166YB UT WOS:000320595800069 PM 23658259 ER PT J AU Meyer, SE Ghimire, S Decker, S Merrill, KR Coleman, CE AF Meyer, Susan E. Ghimire, Sudeep Decker, Samuel Merrill, Keith R. Coleman, Craig E. TI The Ghost of Outcrossing Past in Downy Brome, an Inbreeding Annual Grass SO JOURNAL OF HEREDITY LA English DT Article DE Bromus tectorum; cheatgrass; cleistogamy; selfing breeding system; single nucleotide polymorphic marker ID AVENA-BARBATA; TECTORUM POACEAE; WILD BARLEY; POPULATION DIFFERENTIATION; FESTUCA-MICROSTACHYS; HORDEUM-SPONTANEUM; GENETIC-VARIATION; MATING SYSTEM; EVOLUTION; ISRAEL AB We investigated the frequency of outcrossing in downy brome (Bromus tectorum L.), a cleistogamous weedy annual grass, in both common garden and wild populations, using microsatellite and single nucleotide polymorphic (SNP) markers. In the common garden study, 25 lines with strongly contrasting genotypes were planted in close proximity. We fingerprinted 10 seed progeny from 8 individuals of each line and detected 15 first-generation heterozygotes for a t-value (corrected for cryptic crosses) of 0.0082. Different genotypes were significantly overrepresented as maternal versus paternal parents of heterozygotes, suggesting gender-function-dependent genetic control of outcrossing rates. In 4 wild populations (> 300 individuals each), expected heterozygosity ranged from 0.149 to 0.336, whereas t-values ranged from 0.0027 to 0.0133, indicating high levels of both genetic diversity and inbreeding. Up to a third of the individuals in each population belonged to groups with identical or nearly identical SNP genotypes, whereas many of the remaining individuals were members of loose clusters of apparently related plants that probably represent descendants from past outcrossing events. Strict inbreeding in some lineages within a population with occasional outcrossing in others may be related to positive selection on adaptive syndromes associated with specific inbreeding lineages, or possibly to among-lineage differences in genetic regulation of outcrossing. C1 [Meyer, Susan E.] US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, Provo, UT 84606 USA. [Ghimire, Sudeep; Decker, Samuel; Coleman, Craig E.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Merrill, Keith R.] N Carolina State Univ, Ctr Plant Breeding & Appl Plant Genom, Raleigh, NC 27695 USA. RP Meyer, SE (reprint author), US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, 735 North 500 East, Provo, UT 84606 USA. EM semeyer@xmission.com FU Joint Fire Sciences Program [2007-1-3-10]; US Department of Agriculture, Cooperative State Research, Education, and Extension Service National Research Initiative; Biology of Weedy and Invasive Species Program [2008-35320-18677]; Charles Redd Center for Western Studies FX Joint Fire Sciences Program (2007-1-3-10 to S.E.M.) and the US Department of Agriculture, Cooperative State Research, Education, and Extension Service National Research Initiative, Biology of Weedy and Invasive Species Program (2008-35320-18677 to S.E.M. and C.E.C.), and Annaley Naegle Redd award from the Charles Redd Center for Western Studies (C.E.C.). SNP marker development from the University of Nevada Reno. NR 42 TC 4 Z9 4 U1 3 U2 24 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 J9 J HERED JI J. Hered. PD JUL-AUG PY 2013 VL 104 IS 4 BP 476 EP 490 DI 10.1093/jhered/est019 PG 15 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA 164ZI UT WOS:000320451400004 PM 23564960 ER PT J AU Cronin, MA MacNeil, MD Vu, N Leesburg, V Blackburn, HD Derr, JN AF Cronin, Matthew A. MacNeil, Michael D. Vu, Ninh Leesburg, Vicki Blackburn, Harvey D. Derr, James N. TI Genetic Variation and Differentiation of Bison (Bison bison) Subspecies and Cattle (Bos taurus) Breeds and Subspecies SO JOURNAL OF HEREDITY LA English DT Article DE cattle breeds; genetic variation; microsatellite DNA; plains bison; subspecies; wood bison ID NORTH-AMERICAN BISON; MULTILOCUS GENOTYPE DATA; DOMESTIC CATTLE; PLAINS BISON; POPULATION-STRUCTURE; WOOD BISON; MITOCHONDRIAL-DNA; ZEBU CATTLE; CONSERVATION; MICROSATELLITES AB The genetic relationship of American plains bison (Bison bison bison) and wood bison (Bison bison athabascae) was quantified and compared with that among breeds and subspecies of cattle. Plains bison from 9 herds (N = 136), wood bison from 3 herds (N = 65), taurine cattle (Bos taurus taurus) from 14 breeds (N = 244), and indicine cattle (Bos taurus indicus) from 2 breeds (N = 53) were genotyped for 29 polymorphic microsatellite loci. Bayesian cluster analyses indicate 3 groups, 2 of which are plains bison and 1 of which is wood bison with some admixture, and genetic distances do not show plains bison and wood bison as distinct groups. Differentiation of wood bison and plains bison is also significantly less than that of cattle breeds and subspecies. These and other genetic data and historical interbreeding of bison do not support recognition of extant plains bison and wood bison as phylogenetically distinct subspecies. C1 [Cronin, Matthew A.; Vu, Ninh] Univ Alaska Fairbanks, Sch Nat Resources & Agr Sci, Matanuska Expt Farm, Palmer, AK 99645 USA. [MacNeil, Michael D.] Delta G, Miles City, MT 59301 USA. [Leesburg, Vicki] ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. [Blackburn, Harvey D.] ARS, USDA, Natl Anim Germplasm Program, Ft Collins, CO 80523 USA. [Derr, James N.] Texas A&M Univ, Dept Vet Pathobiol, College Stn, TX 77845 USA. RP Cronin, MA (reprint author), Univ Alaska Fairbanks, Sch Nat Resources & Agr Sci, Matanuska Expt Farm, 1509 South Georgeson Dr, Palmer, AK 99645 USA. EM macronin@alaska.edu FU State of Alaska Department of Fish and Game; U.S. Department of Agriculture, Agricultural Research Service Fort Keogh Livestock and Range Research Laboratory FX State of Alaska Department of Fish and Game and the U.S. Department of Agriculture, Agricultural Research Service Fort Keogh Livestock and Range Research Laboratory. NR 55 TC 6 Z9 7 U1 1 U2 33 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-1503 J9 J HERED JI J. Hered. PD JUL-AUG PY 2013 VL 104 IS 4 BP 500 EP 509 DI 10.1093/jhered/est030 PG 10 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA 164ZI UT WOS:000320451400006 PM 23667052 ER PT J AU Edwards, HD Anderson, RC Taylor, TM Miller, RK Hardin, MD Nisbet, DJ Krueger, NA Smith, SB AF Edwards, H. D. Anderson, R. C. Taylor, T. M. Miller, R. K. Hardin, M. D. Nisbet, D. J. Krueger, N. A. Smith, S. B. TI Interactions Between Oil Substrates and Glucose on Pure Cultures of Ruminal Lipase-Producing Bacteria SO LIPIDS LA English DT Article DE Lipid chemistry; Lipases; Lipid metabolism; Fatty acid metabolism ID CONJUGATED LINOLEIC-ACID; PERFORMANCE LIQUID-CHROMATOGRAPHY; CORONARY-HEART-DISEASE; LACTATING DAIRY-COWS; FATTY-ACIDS; ANAEROVIBRIO-LIPOLYTICA; IN-VITRO; ANAEROBIC-BACTERIA; DIETARY-FAT; RUMEN AB The hydrolysis of free fatty acids from lipids is a prerequisite for biohydrogenation, a process that effectively saturates free fatty acids. Anaerovibrio lipolyticus 5s and Butyrivibrio fibrisolvens have long been thought to be the major contributors to ruminal lipolysis; however, Propionibacterium avidum and acnes recently have been identified as contributing lipase activity in the rumen. In order to further characterize the lipase activity of these bacterial populations, each was grown with three different lipid substrates, olive oil, corn oil, and flaxseed oil (3 %). Because different finishing rations contain varying levels of glycogen (a source of free glucose) this study also documented the effects of glucose on lipolysis. P. avidum and A. lipolyticus 5s demonstrated the most rapid rates (P < 0.05) of lipolysis for cultures grown with olive oil and flaxseed oil, respectively. A. lipolyticus, B. fibrisolvens, and P. avidum more effectively hydrolyzed flaxseed oil than olive oil or corn oil, especially in the presence of 0.02 % glucose. Conversely, P. acnes hydrolyzed corn oil more readily than olive oil or flaxseed oil and glucose had no effect on lipolytic rate. Thus, these bacterial species demonstrated different specificities for oil substrates and different sensitivities to glucose. C1 [Edwards, H. D.; Anderson, R. C.; Nisbet, D. J.; Krueger, N. A.] ARS, USDA, College Stn, TX USA. [Taylor, T. M.; Miller, R. K.; Hardin, M. D.; Smith, S. B.] 2471 Texas A&M Univ, Dept Anim Sci, Kleberg Ctr 338, College Stn, TX 77843 USA. [Hardin, M. D.] Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. RP Smith, SB (reprint author), 2471 Texas A&M Univ, Dept Anim Sci, Kleberg Ctr 338, College Stn, TX 77843 USA. EM sbsmith@tamu.edu RI Smith, Stephen/H-7046-2014; Taylor, Thomas/P-2076-2015 OI Smith, Stephen/0000-0002-2779-8281; Taylor, Thomas/0000-0003-4191-5285 FU USDA, Cooperative State Research, Education and Extension Service (CSREES) [2009-51110-05852] FX This research was supported in part by USDA, Cooperative State Research, Education and Extension Service (CSREES) Grant Number 2009-51110-05852. The expert technical assistance of Jackie Kotzur is greatly appreciated. NR 44 TC 1 Z9 1 U1 0 U2 22 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0024-4201 J9 LIPIDS JI Lipids PD JUL PY 2013 VL 48 IS 7 BP 749 EP 755 DI 10.1007/s11745-013-3793-3 PG 7 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA 170OD UT WOS:000320861600010 PM 23609414 ER PT J AU Marvasi, M Cox, CE Xu, YM Noel, JT Giovannoni, JJ Teplitski, M AF Marvasi, Massimiliano Cox, Clayton E. Xu, Yimin Noel, Jason T. Giovannoni, James J. Teplitski, Max TI Differential Regulation of Salmonella Typhimurium Genes Involved in O-Antigen Capsule Production and Their Role in Persistence Within Tomato Fruit SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID VIVO EXPRESSION TECHNOLOGY; ENTERICA SEROVAR TYPHIMURIUM; ALMOND ORCHARD; PLANTS; COLONIZATION; COLI; METABOLITE; MUTATIONS; INFECTION; OUTBREAK AB Enteric pathogens, including non-typhoidal Salmonella spp. and enterovirulent Escherichia coli, are capable of persisting and multiplying within plants. Yet, little is still known about the mechanisms of these interactions. This study identified the Salmonella yihT gene (involved in synthesis of the O-antigen capsule) as contributing to persistence in immature tomato fruit. Deletion of yihT reduced competitive fitness of S. enterica sv. Typhimurium in green (but not ripe, regardless of color) tomato fruit by approximately 3 logs. The yihT recombinase-based in vivo expression technology (RIVET) reporter was strongly activated in unripe tomato fruit, and fitness of the mutant inversely correlated with the level of the yihT gene expression. Expression of yihT in mature tomato fruit was low, and yihT did not affect competitive fitness within mature fruit. To better understand the molecular basis of the phenotype, behaviors of the yihT RIVET reporter and the yihT mutant were tested in tomato fruit defective in ethylene signaling. These experiments suggest a role for functional ethylene-mediated signaling in the persistence of Salmonella spp. within tomato fruit. Furthermore, jasmonic acid and its precursors strongly reduced expression of yihT. C1 [Marvasi, Massimiliano; Cox, Clayton E.; Noel, Jason T.; Teplitski, Max] Univ Florida, Soil & Water Sci Dept, Genet Inst, IFAS, Gainesville, FL 32611 USA. [Xu, Yimin; Giovannoni, James J.] Cornell Univ, USDA ARS, Ithaca, NY 14853 USA. [Xu, Yimin; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Teplitski, M (reprint author), Univ Florida, Soil & Water Sci Dept, Genet Inst, IFAS, Rm330E,2033 Mowry Rd, Gainesville, FL 32611 USA. EM maxtep@ufl.edu FU United States Department of Agriculture (USDA)-NRI AFRI [2011-67017-30127]; Center for Produce Safety, University of California-Davis [201300842-01]; California Department of Food and Agriculture's Specialty Crop Block Grant Program [SCB12064]; USDA NTFA postdoctoral fellowship [CRIS 0229689] FX This research was supported by the United States Department of Agriculture (USDA)-NRI AFRI grant 2011-67017-30127 (to M. Teplitski and J. J. Giovannoni). Construction of the phoN mutant was funded as a part of the project supported by the Center for Produce Safety, University of California-Davis (agreement number 201300842-01) and the California Department of Food and Agriculture's Specialty Crop Block Grant Program (SCB12064). C. E. Cox gratefully acknowledges USDA NTFA postdoctoral fellowship CRIS 0229689. We thank M. Moraes for assistance with designing primers to construct CEC1000. NR 33 TC 8 Z9 8 U1 0 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD JUL PY 2013 VL 26 IS 7 BP 793 EP 800 DI 10.1094/MPMI-09-12-0208-R PG 8 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA 164LO UT WOS:000320411800008 PM 23489058 ER PT J AU Gambetta, GA McElrone, AJ Matthews, MA AF Gambetta, Gregory A. McElrone, Andrew J. Matthews, Mark A. TI Genomic DNA-based absolute quantification of gene expression in Vitis SO PHYSIOLOGIA PLANTARUM LA English DT Article ID REAL-TIME PCR; RT-PCR; SYSTEMATIC VALIDATION; PIERCES-DISEASE; TRANSCRIPTION; VINIFERA; AMPLIFICATION; REFERENCES; RELEVANCE; NOISE AB Many studies in which gene expression is quantified by polymerase chain reaction represent the expression of a gene of interest (GOI) relative to that of a reference gene (RG). Relative expression is founded on the assumptions that RG expression is stable across samples, treatments, organs, etc., and that reaction efficiencies of the GOI and RG are equal; assumptions which are often faulty. The true variability in RG expression and actual reaction efficiencies are seldom determined experimentally. Here we present a rapid and robust method for absolute quantification of expression in Vitis where varying concentrations of genomic DNA were used to construct GOI standard curves. This methodology was utilized to absolutely quantify and determine the variability of the previously validated RG ubiquitin (VvUbi) across three test studies in three different tissues (roots, leaves and berries). In addition, in each study a GOI was absolutely quantified. Data sets resulting from relative and absolute methods of quantification were compared and the differences were striking. VvUbi expression was significantly different in magnitude between test studies and variable among individual samples. Absolute quantification consistently reduced the coefficients of variation of the GOIs by more than half, often resulting in differences in statistical significance and in some cases even changing the fundamental nature of the result. Utilizing genomic DNA-based absolute quantification is fast and efficient. Through eliminating error introduced by assuming RG stability and equal reaction efficiencies between the RG and GOI this methodology produces less variation, increased accuracy and greater statistical power. C1 [Gambetta, Gregory A.; Matthews, Mark A.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [McElrone, Andrew J.] Univ Calif Davis, ARS, USDA, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Gambetta, GA (reprint author), Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. EM gagambetta@ucdavis.edu RI Matthews, Mark/A-5714-2010 FU American Vineyard Foundation; U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service [2005-34442-15841, 2010-65114-20368] FX The authors of this work would like to thank Sarah Greenleaf, Ryan Leininger and Beringer Vineyards for facilitating the field studies. Critical input to this project was provided by Simone Castellarin and Alan B. Rose. All greenhouse-grown grapevines were kindly donated by Cal Western Nurseries, Visalia, CA, USA. This work was funded in part by American Vineyard Foundation grants to A. J. M. and U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service (grants no. 2005-34442-15841 and 2010-65114-20368) to M. A. M. NR 32 TC 2 Z9 2 U1 1 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD JUL PY 2013 VL 148 IS 3 SI SI BP 334 EP 343 DI 10.1111/ppl.12014 PG 10 WC Plant Sciences SC Plant Sciences GA 164EY UT WOS:000320393000003 PM 23198692 ER PT J AU Yang, TB Peng, H Whitaker, BD Jurick, WM AF Yang, Tianbao Peng, Hui Whitaker, Bruce D. Jurick, Wayne M. TI Differential expression of calcium/calmodulin-regulated SlSRs in response to abiotic and biotic stresses in tomato fruit SO PHYSIOLOGIA PLANTARUM LA English DT Article ID CALMODULIN-BINDING PROTEINS; GENE-EXPRESSION; CALCIUM-CHLORIDE; SALICYLIC-ACID; SIGNAL-TRANSDUCTION; ETHYLENE PRODUCTION; BOTRYTIS-CINEREA; SHELF-LIFE; ARABIDOPSIS; PLANTS AB Calcium has been shown to enhance stress tolerance, maintain firmness and reduce decay in fruits. Previously we reported that seven tomato SlSRs encode calcium/calmodulin-regulated proteins, and that their expressions are developmentally regulated during fruit development and ripening, and are also responsive to ethylene. To study their expressions in response to stresses encountered during postharvest handling, tomato fruit at the mature-green stage was subjected to chilling and wounding injuries, infected with Botrytis cinerea and treated with salicylic acid or methyl jasmonate. Gene expression studies revealed that the seven SlSRs differentially respond to different stress signals. SlSR2 was the only gene upregulated by all the treatments. SlSR4 acted as a late pathogen-induced gene; it was upregulated by salicylic acid and methyl jasmonate, but downregulated by cold treatment. SlSR3L was cold- and wound-responsive and was also induced by salicylic acid. SlSR1 and SlSR1L were repressed by cold, wounding and pathogen infection, but were upregulated by salicylic acid and methyl jasmonate. Overall, results of these expression studies indicate that individual SlSRs have distinct roles in responses to the specific stress signals, and SlSRs may act as a coordinator(s) connecting calcium-mediated signaling with other stress signal transduction pathways during fruit ripening and storage. C1 [Yang, Tianbao; Peng, Hui; Whitaker, Bruce D.; Jurick, Wayne M.] ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Yang, TB (reprint author), ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM tianbao.yang@ars.usda.gov FU USDA-ARS [1245-43000-012-00D] FX We thank Ernest Paroczay and Luhong Huang for their excellent technical support. Use of a company or product name by the U. S. Department of Agriculture does not imply approval or recommendation of the product to the exclusion of others that may also be suitable. This research was funded by USDA-ARS project no. 1245-43000-012-00D. NR 59 TC 14 Z9 18 U1 1 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD JUL PY 2013 VL 148 IS 3 SI SI BP 445 EP 455 DI 10.1111/ppl.12027 PG 11 WC Plant Sciences SC Plant Sciences GA 164EY UT WOS:000320393000011 PM 23368882 ER PT J AU Bonde, MR Nester, SE Berner, DK AF Bonde, M. R. Nester, S. E. Berner, D. K. TI Effects of Frequency of "Extreme" Temperature Highs on Development of Soybean Rust SO PHYTOPATHOLOGY LA English DT Article ID PHAKOPSORA-PACHYRHIZI; UNITED-STATES; INFECTION; SUSCEPTIBILITY; PLANT; AGE AB Previously, we hypothesized that summer "extreme" diurnal temperature highs in the southeastern United States were responsible for the yearly absence or delay of soybean rust development until fall. Utilizing temperature-controlled growth chambers, a diurnal temperature pattern of 33 degrees C high and 20 degrees C low reduced urediniospore production by 81%. However, that study did not consider the influence of frequency of extreme temperatures on soybean rust. We now report that a temperature high of 35 degrees C for 1 h on three consecutive days, initiated 15 days after inoculation, when lesions had formed, reduced urediniospore production by 50% and required 9 to 12 days for sporulation to resume once the extreme temperature highs ceased. Furthermore, three consecutive days in which the temperature high was 37 degrees C, beginning immediately after inoculation and subsequent dew period, reduced lesion numbers by 60%. The combined effects of reduced numbers of lesions and urediniospores per lesion caused by extreme temperature highs can account for observed absence or delay of soybean rust development in the southeastern United States until fall. A comparison of frequency of extreme temperature highs with numbers of counties reporting presence of soybean rust from 2005 to 2012 verified that extreme temperature highs may be largely responsible for absence or delay of soybean rust development. This is the first report showing the effect of frequency of extreme temperature highs on development of soybean rust. Because the south-to-north progression of soybean rust is required for the disease to occur in the major soybean-production regions of the United States, temperatures in the southeastern United States have a major effect on the entire U.S. soybean industry. C1 [Bonde, M. R.; Nester, S. E.; Berner, D. K.] USDA ARS, Foreign Dis Weed Sci Res Unit, Frederick, MD 21702 USA. RP Bonde, MR (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Frederick, MD 21702 USA. EM Morris.bonde@ars.usda.gov NR 28 TC 3 Z9 3 U1 0 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2013 VL 103 IS 7 BP 708 EP 716 DI 10.1094/PHYTO-09-12-0250-R PG 9 WC Plant Sciences SC Plant Sciences GA 168ZI UT WOS:000320746100006 PM 23425238 ER PT J AU Asalf, B Gadoury, DM Tronsmo, AM Seem, RC Cadle-Davidson, L Brewer, MT Stensvand, A AF Asalf, Belachew Gadoury, David M. Tronsmo, Anne Marte Seem, Robert C. Cadle-Davidson, Lance Brewer, Marin Talbot Stensvand, Arne TI Temperature Regulates the Initiation of Chasmothecia in Powdery Mildew of Strawberry SO PHYTOPATHOLOGY LA English DT Article DE Fragaria x ananassa; Sphaerotheca macularis ID SPHAEROTHECA-MACULARIS; UNCINULA-NECATOR; ERYSIPHE-NECATOR; MATING-TYPE; CLEISTOTHECIA; HETEROTHALLISM; SURVIVAL; GROWTH; LEAVES; FUNGUS AB The formation of chasmothecia by the strawberry powdery mildew pathogen (Podosphaera aphanis) is widespread but often sporadic throughout the range of strawberry cultivation. In some production regions, notably in warmer climates, chasmothecia are reportedly rare. We confirmed that the pathogen is heterothallic, and that initiation of chasmothecia is not only dependent upon the presence of isolates of both mating types but also largely suppressed at temperatures >13 degrees C. Compared with incubation at a constant temperature of 25 degrees C, progressively more chasmothecia were initiated when temperatures were decreased to 13 degrees C for progressively longer times. At lower temperatures, production of chasmothecia was associated with a decline in but not total cessation of conidial formation, and pairings of compatible isolates sporulated abundantly at 25 degrees C. We developed mating-type markers specific to P. aphanis and used these to confirm the presence of both mating types in populations that had not yet initiated chasmothecia. The geographic discontinuity of chasmothecia production and the sporadic and seemingly unpredictable appearance of chasmothecia in P. aphanis are possibly due to the combined influence of heterothallism and suppression of chasmothecia formation by high temperatures. C1 [Asalf, Belachew; Tronsmo, Anne Marte] Norwegian Univ Life Sci, Dept Plant & Environm Sci, N-1432 As, Norway. [Asalf, Belachew; Stensvand, Arne] Norwegian Inst Agr & Environm Res Bioforsk, Plant Hlth & Plant Protect Div, N-1432 As, Norway. [Gadoury, David M.; Seem, Robert C.] Cornell Univ, Dept Plant Pathol, New York State Agr Expt Stn, Geneva, NY 14456 USA. [Cadle-Davidson, Lance] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. [Brewer, Marin Talbot] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. RP Asalf, B (reprint author), Norwegian Univ Life Sci, Dept Plant & Environm Sci, N-1432 As, Norway. EM belachew.asalf.tadesse@umb.no FU Research Council of Norway; Norwegian University of Life Sciences; Bioforsk; United States Department of Agriculture Crops at Risk competitive grants program; North American Strawberry Growers Association FX This work was supported by the Research Council of Norway, the Norwegian University of Life Sciences, and Bioforsk. Additional support for the work was provided through the United States Department of Agriculture Crops at Risk competitive grants program, and the competitive grants program of the North American Strawberry Growers Association. We thank A. Dobson (Bioforsk) and M. J. Welser (Cornell University) for their excellent technical assistance. NR 31 TC 5 Z9 5 U1 3 U2 23 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2013 VL 103 IS 7 BP 717 EP 724 DI 10.1094/PHYTO-09-12-0252-R PG 8 WC Plant Sciences SC Plant Sciences GA 168ZI UT WOS:000320746100007 PM 23384856 ER PT J AU Attanayake, RN Carter, PA Jiang, DH del Rio-Mendoza, L Chen, WD AF Attanayake, Renuka N. Carter, Patrick A. Jiang, Daohong del Rio-Mendoza, Luis Chen, Weidong TI Sclerotinia sclerotiorum Populations Infecting Canola from China and the United States Are Genetically and Phenotypically Distinct SO PHYTOPATHOLOGY LA English DT Article ID FUNGAL PLANT PATHOGEN; MYCELIAL COMPATIBILITY GROUPS; MICROSATELLITE MARKERS; ALLELE FREQUENCIES; FIELD POPULATIONS; RESISTANCE; DIVERSITY; TRIFOLIORUM; FUNGICIDES; GENE AB Genetic and phenotypic diversity and population differentiation of Sclerotinia sclerotiorum isolates infecting canola from China and the United States were investigated. Genetic diversity was assessed with eight microsatellite markers and mycelial compatibility groups (MCGs). Phenotypic diversity was assessed with sensitivity to three fungicides, production of oxalate and sclerotia, growth rate, and virulence on two canola cultivars. No shared MCGs or multilocus haplotypes were detected between the two populations, and populations differed significantly (P < 0.001). Recombination was detected in both populations but was greater in the Chinese population. A polymerase chain reaction detection assay showed that 60% of the isolates were inversion-plus at the mating type locus. The two populations differed significantly (P < 0.05) for all of the phenotypic traits except for sensitivity to fungicide fluazinam and virulence. Isolates in the Chinese population were unique in several aspects. Despite the phenotypic differentiation, heritabilities of the phenotypic traits were similar for both populations. Significant correlations were found among five phenotypic traits. Cross resistance to benomyl and iprodione was detected. Virulence was not significantly correlated with any other phenotypic trait and had the least heritability. However, both populations were equally virulent on either a susceptible or a moderately resistant canola cultivars. C1 [Attanayake, Renuka N.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Carter, Patrick A.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Jiang, Daohong] Huazhong Agr Univ, Dept Plant Protect, Wuhan, Hubei, Peoples R China. [del Rio-Mendoza, Luis] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Chen, Weidong] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. RP Chen, WD (reprint author), Washington State Univ, USDA ARS, Pullman, WA 99164 USA. EM w-chen@wsu.edu FU United States Department of Agriculture Agricultural Research Service (USDA-ARS) National Sclerotinia Initiative FX The project was funded, in part, by the United States Department of Agriculture Agricultural Research Service (USDA-ARS) National Sclerotinia Initiative. We thank F. Dugan of USDA-ARS and D. Johnson of Washington States University for critically reviewing the manuscript, N. Dasgupta of Washington State University for consultation on statistical analysis, D. See of USDA-ARS for providing the genotyping facility, and K. V Subbarao of the University of California-Davis for providing prepublication information on invasion of MAT loci. NR 57 TC 18 Z9 19 U1 1 U2 40 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUL PY 2013 VL 103 IS 7 BP 750 EP 761 DI 10.1094/PHYTO-07-12-0159-R PG 12 WC Plant Sciences SC Plant Sciences GA 168ZI UT WOS:000320746100011 PM 23464902 ER PT J AU Telugu, BP Adachi, K Schlitt, JM Ezashi, T Schust, DJ Roberts, RM Schulz, LC AF Telugu, B. P. Adachi, K. Schlitt, J. M. Ezashi, T. Schust, D. J. Roberts, R. M. Schulz, L. C. TI Comparison of extravillous trophoblast cells derived from human embryonic stem cells and from first trimester human placentas SO PLACENTA LA English DT Article DE Extravillous trophoblast; Human embryonic stem cell; BMP4; Trophoblast; Pre-eclampsia ID CYTOTROPHOBLAST DIFFERENTIATION; MONOCLONAL-ANTIBODIES; TRANSCRIPTION FACTORS; GENE-EXPRESSION; SUBUNIT GENE; NITRIC-OXIDE; HLA-G; PREECLAMPSIA; INVASION; PREGNANCY AB Introduction: Preeclampsia and other placental pathologies are characterized by a lack of spiral artery remodeling associated with insufficient invasion by extravillous trophoblast cells (EVT). Because trophoblast invasion occurs in early pregnancy when access to human placental tissue is limited, there is a need for model systems for the study of trophoblast differentiation and invasion. Human embryonic stem cells (hESC) treated with BMP4- differentiate to trophoblast, and express HLA-G, a marker of EVT. The goals of the present study were to further characterize the HLA-G(+) cells derived from BMP4-treated hESC, and determine their suitability as a model. Methods: HESC were treated with BMP4 under 4% or 20% oxygen and tested in Matrigel invasion chambers. Both BMP4-treated hESC and primary human placental cells were separated into HLA-G(+) and HLA-G(-)/TACSTD2(+) populations with immunomagnetic beads and expression profiles analyzed by microarray. Results: There was a 10-fold increase in invasion when hESC were BMP4-treated. There was also an independent, stimulatory effect of oxygen on this process. Invasive cells expressed trophoblast marker KRT7, and the majority were also HLA-G(+). Gene expression profiles revealed that HLA-G(+), BMP4-treated hESC were similar to, but distinct from, HLA-G(+) cells isolated from first trimester placentas. Whereas HLA-G(+) and HLA-G(-) cells from first trimester placentas had highly divergent gene expression profiles, HLA-G(+) and HLA-G(-) cells from BMP4-treated hESC had somewhat similar profiles, and both expressed genes characteristic of early trophoblast development. Conclusions: We conclude that hESC treated with BMP4 provide a model for studying transition to the EVT lineage. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Telugu, B. P.] Univ Maryland, College Pk, MD 20742 USA. [Telugu, B. P.] USDA, Anim Biosci & Biotechnol Lab, ANRI, ARS, Beltsville, MD 20705 USA. [Adachi, K.; Schlitt, J. M.; Schust, D. J.; Schulz, L. C.] Univ Missouri, Dept Obstet Gynecol & Womens Hlth, Columbia, MO 65212 USA. [Ezashi, T.; Roberts, R. M.] Univ Missouri, Bond Life Sci Ctr, Columbia, MO 65211 USA. RP Schulz, LC (reprint author), NW509 Hlth Sci Ctr,1 Hosp Dr, Columbia, MO 65212 USA. EM schulzL@missouri.edu OI Schulz, Laura/0000-0001-8722-6616 FU Preeclampsia Foundation; Institute for Clinical and Translational Science; NIH [R01HD067759] FX This work is partially supported by funds from the Preeclampsia Foundation "Vision Grant" Award, and Institute for Clinical and Translational Science "Pilot Grant" to BT, and NIH R01HD067759 to RMR, TE, LCS, & DJS. We thank Kim Livingston and Sunilima Sinha for technical assistance and Takeshi Nagamatsu and Mitsuyoshi Amita for helpful discussions. NR 57 TC 11 Z9 11 U1 1 U2 10 PU W B SAUNDERS CO LTD PI LONDON PA 32 JAMESTOWN RD, LONDON NW1 7BY, ENGLAND SN 0143-4004 J9 PLACENTA JI Placenta PD JUL PY 2013 VL 34 IS 7 BP 536 EP 543 DI 10.1016/j.placenta.2013.03.016 PG 8 WC Developmental Biology; Obstetrics & Gynecology; Reproductive Biology SC Developmental Biology; Obstetrics & Gynecology; Reproductive Biology GA 170CL UT WOS:000320827100004 PM 23631809 ER PT J AU Ploetz, RC Hulcr, J Wingfield, MJ de Beer, ZW AF Ploetz, Randy C. Hulcr, Jiri Wingfield, Michael J. de Beer, Z. Wilhelm TI Destructive Tree Diseases Associated with Ambrosia and Bark Beetles: Black Swan Events in Tree Pathology? SO PLANT DISEASE LA English DT Article ID DUTCH ELM DISEASE; COLEOPTERA-CURCULIONIDAE SCOLYTINAE; LAUREL WILT DISEASE; PLATYPUS-QUERCIVORUS COLEOPTERA; SOUTHEASTERN UNITED-STATES; MOUNTAIN PINE-BEETLE; ESSENTIAL OIL LURES; WALNUT TWIG BEETLE; RAFFAELEA-LAURICOLA; XYLEBORUS-GLABRATUS C1 [Ploetz, Randy C.] Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. [Hulcr, Jiri] Univ Florida, Sch Conservat & Forest Resources, Gainesville, FL 32611 USA. [Hulcr, Jiri] Univ Florida, USDA, US Forest Serv, Gainesville, FL 32611 USA. [Wingfield, Michael J.; de Beer, Z. Wilhelm] Univ Pretoria, Dept Microbiol & Plant Pathol, Forest & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa. [de Beer, Z. Wilhelm] Univ Pretoria, Dept Microbiol, Forest & Agr Biotechnol Inst, ZA-0002 Pretoria, South Africa. RP Ploetz, RC (reprint author), Univ Florida, Ctr Trop Res & Educ, Homestead, FL 33031 USA. EM kelly12@ufl.edu RI Wingfield, Michael/A-9473-2008; de Beer, Z. Wilhelm/B-6353-2008 OI de Beer, Z. Wilhelm/0000-0001-9758-8987 FU USDA [2008-31135-19505, 2009-51181-05915] FX We thank Whitney Cranshaw, Diana Six, Jason Smith, Ned Tisserat, Mark Windham, two anonymous reviewers for comments, and Joan Webber for valuable input and published materials on Dutch elm disease. IPM Images (http://www.ipmimages.org/) are acknowledged for Figures 4A (5458858), 5A (5328093), 5B (5328043), 5D (5323055), 5E (5463338), 5F (5482204), 5H (5322045), 5J (5329078), and 8A (5406069); PaDIL (http://www.padil.gov.au/) Figures 5C (45657, Sarah McCaffrey, Museum Victoria, Australia), 5G (42573 Sarah McCaffrey, Museum Victoria, Australia), 51 (33462, Justin Bartlett), 5K (7914, Ken Walker, Museum Victoria, Australia), and 5L (8239, Ken Walker, Museum Victoria, Australia); Ned Tisserat (University of Colorado) for Figures 8B and C; the USDA Forest Service (http://www.fs.fed.us/r8/foresthealth/laurelwilt/dist_map.shtml) for Figure 10; and Stanley Freeman (Volcani Center, Israel) for the inset photo in Figure 11. IPM Images is a joint project of the Center for Invasive Species and Ecosystem Health, Colorado State University, USDA National Institute of Food and Agriculture, Southern Plant Diagnostic Network. The University of Georgia - Warnell School of Forestry and Natural Resources and College of Agricultural and Environmental Sciences). The PaDIL site contains images that are licensed to the public under the Creative Commons Attribution 3.0 Australian License. Some of RCP's research on laurel wilt was supported by USDA grants 2008-31135-19505 and 2009-51181-05915. We dedicate this review to the memory of Julian Gilbert Leach, a pioneer in our understanding of arthropod-vectors of plant pathogens and the author of the influential text, Insect Transmission of Plant Diseases (54,101). NR 177 TC 41 Z9 42 U1 5 U2 73 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 856 EP 872 DI 10.1094/PDIS-01-13-0056-FE PG 17 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800001 ER PT J AU Newcomb, M Acevedo, M Bockelman, HE Brown-Guedira, G Goates, BJ Jackson, EW Jin, Y Njau, P Rouse, MN Singh, D Wanyera, R Bonman, JM AF Newcomb, M. Acevedo, M. Bockelman, H. E. Brown-Guedira, G. Goates, B. J. Jackson, E. W. Jin, Y. Njau, P. Rouse, M. N. Singh, D. Wanyera, R. Bonman, J. M. TI Field Resistance to the Ug99 Race Group of the Stem Rust Pathogen in Spring Wheat Landraces SO PLANT DISEASE LA English DT Article ID F-SP TRITICI; GENETIC DIVERSITY; GEOGRAPHIC-DISTRIBUTION; MOLECULAR MARKERS; AESTIVUM L.; LEAF RUST; US WHEAT; GERMPLASM; SR24; CULTIVARS AB Wheat landraces provide a source of genetic variability for breeding. The emergence and spread of highly virulent races of the stem rust pathogen (Ug99 race group of Puccinia graminis f. sp. tritici) threaten wheat production globally. Spring wheat landraces were screened for resistance in eight field seasons at the Kenya Agricultural Research Institute, Njoro, where the Ug99 race group has become endemic. Accessions showing resistance in one season were retested and screened with molecular markers associated with resistance genes Sr2, ST24, Sr36, and Lr34/Yr18; two height-reducing genes; and a photoperiod insensitivity allele. Of 2,509 accessions tested, 278 were categorized as resistant based on results from at least two seasons. Of these resistant accessions, 32 were positive for one or more markers for Sr2, Sr36, Rht-B1b, or Rht-D1b, indicating that they do not fit the definition of "landrace" because these genes were likely introduced via modern breeding practices. Thus, 246 resistant "landrace" accessions were identified. Of countries with more than five tested accessions, Afghanistan, Iran, Portugal, Ethiopia, Uzbekistan, Greece, Tajikistan, Bosnia and Herzegovina, and Serbia had at least 10% of tested accessions that were resistant to the Ug99 race group. Future research will characterize the resistance to determine its novelty and incorporate novel genes into improved lines. C1 [Newcomb, M.; Bockelman, H. E.; Goates, B. J.; Jackson, E. W.; Bonman, J. M.] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. [Acevedo, M.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Brown-Guedira, G.] ARS, USDA, Eastern Reg Genotyping Lab, Raleigh, NC 27695 USA. [Jin, Y.] Univ Minnesota, USDA, ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Njau, P.; Wanyera, R.] Kenya Agr Res Inst, Njoro, Kenya. [Rouse, M. N.] ARS, USDA, Cereal Dis Lab, Aberdeen, ID 83210 USA. [Singh, D.] Univ Sydney, Plant Breeding Inst, Sydney, NSW 2006, Australia. RP Newcomb, M (reprint author), ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID 83210 USA. EM Maria.Newcomb@ars.usda.gov RI Rouse, Matthew/G-5474-2011 NR 59 TC 12 Z9 12 U1 1 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 882 EP 890 DI 10.1094/PDIS-02-12-0200-RE PG 9 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800003 ER PT J AU Seifers, DL Tatineni, S French, R AF Seifers, Dallas L. Tatineni, Satyanarayana French, Roy TI Variants of Triticum mosaic virus Isolated From Wheat in Colorado Show Divergent Biological Behavior SO PLANT DISEASE LA English DT Article ID HIGH-PLAINS VIRUS; WINTER-WHEAT; CURL MITE; IDENTIFICATION; VECTOR AB Triticum mosaic virus (TriMV) is a recently discovered virus infecting wheat. We compared Colorado isolates C10-492 and C11-775 with the 06-123 isolate. Comparisons were made using enzyme-linked immunosorbent assay (ELISA), infectivity assay, host range, thy weight (DW), inoculation of 'Mace' wheat with temperature-sensitive resistance to Wheat streak mosaic virus, and the deduced amino acid sequence of the coat proteins (CP) and P1 proteins. Both C10-492 and C11-775 infected 'Gallatin' barley and, when compared with 06-123, had significantly reduced ELISA values and virus titers in wheat. Both Colorado isolates caused symptomless infections in Mace, whereas 06-123 caused mosaic symptoms. The amino acid sequences of the CP differed by two and one amino acids for C10-492 and C11-775, respectively, compared with 06-123. The sequence of C10-492 differed from C11-775 by one amino acid. The P1 amino acid sequence of C10-492 and C11-775 differed from 06-123 by three and one amino acids, respectively. The C10-492 and C11-775 isolates reduced DW significantly in 'Karl 92' but significantly less than 06-123. In '2317' wheat, the Colorado isolates did not consistently cause significant reduction in DW, while 06-123 did. The data collectively indicate that C10-492 and C11-775 are isolates of TriMV showing biological behavior diverse from that of 06-123. C1 [Seifers, Dallas L.] Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. [Tatineni, Satyanarayana; French, Roy] Univ Nebraska, USDA, ARS, Lincoln, NE 68583 USA. [Tatineni, Satyanarayana; French, Roy] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Seifers, DL (reprint author), Kansas State Univ, Agr Res Ctr Hays, Hays, KS 67601 USA. EM dseifers@ksu.edu FU Agriculture and Food Research Initiative Competitive Grants Program from the National Institute of Food and Agriculture [2010-85605-20546] FX Funding for this work was provided by the Agriculture and Food Research Initiative Competitive Grants Program Grant Number 2010-85605-20546 from the National Institute of Food and Agriculture. We thank G. Hein and S. Wegulo for their critical reviews of this manuscript, J. Ackerman for his valuable assistance during all phases of these studies, and C. Seaman for data analyses. NR 22 TC 2 Z9 2 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 903 EP 911 DI 10.1094/PDIS-10-12-0925-RE PG 9 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800006 ER PT J AU Baumgartner, K Fujiyoshi, PT Travadon, R Castlebury, LA Wilcox, WF Rolshausen, PE AF Baumgartner, Kendra Fujiyoshi, Phillip T. Travadon, Renaud Castlebury, Lisa A. Wilcox, Wayne F. Rolshausen, Philippe E. TI Characterization of Species of Diaporthe from Wood Cankers of Grape in Eastern North American Vineyards SO PLANT DISEASE LA English DT Article ID PHOMOPSIS-VITICOLA; LEAF-SPOT; UNITED-STATES; SOUTH-AFRICA; EUTYPA-LATA; PEACH-TREES; DIEBACK; DISEASE; CANE; IDENTIFICATION AB In eastern North America, Phomopsis cane and leaf spot, caused by Phomopsis viticola, is a foliar disease of grape but, in the Mediterranean climate of western North America, P viticola is primarily associated with wood cankers, along with other Diaporthe spp. To determine the identity of wood-infecting Diaporthe spp. in eastern North America, 65 isolates were cultured from 190 wood-canker samples from 23 vineyards with a history of Phomopsis cane and leaf spot. Identification of 29 representative isolates was based initially on morphology, followed by phylogenetic analyses of DNA sequences of the ribosomal DNA internal transcribed spacer region, elongation factor subunit 1-alpha, and actin in comparison with those of type specimens. Three species were identified: P. viticola, P. fukushii, and Diaporthe eres. Inoculations onto woody stems of potted Vitis labruscana 'Concord' and V vinifera 'Chardonnay' showed that D. eres and P. fukushii were pathogenic (mean lesion lengths of 7.4 and 7.1 mm, respectively, compared with 3.5 mm for noninoculated controls) but significantly less so than wood-canker and leaf-spot isolates of P. viticola (13.5 mm). All three species infected pruning wounds of Concord and Chardonnay in the field. Our finding of pathogenic, wood-infecting Diaporthe spp. in all 23 vineyards suggests a frequent co-occurrence of the foliar symptoms of Phomopsis cane and leaf spot and wood cankers, although the latter are not always due to P. viticola. C1 [Baumgartner, Kendra; Fujiyoshi, Phillip T.] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. [Travadon, Renaud] Univ Calif Davis, Dept Plant Pathol, Davis, CA USA. [Castlebury, Lisa A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. [Wilcox, Wayne F.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14853 USA. [Rolshausen, Philippe E.] Univ Calif Riverside, Dept Plant Pathol & Microbiol, Riverside, CA 92521 USA. RP Baumgartner, K (reprint author), ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA 95616 USA. EM kbaumgartner@ucdavis.edu FU USDA, National Institute of Food and Agriculture [2008-51100-19334] FX Research was funded by grant number 2008-51100-19334 to K. Baumgartner and P. E. Rolshausen from the USDA, National Institute of Food and Agriculture. USDA is an equal opportunity provider and employer. NR 48 TC 13 Z9 13 U1 0 U2 23 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 912 EP 920 DI 10.1094/PDIS-04-12-0357-RE PG 9 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800007 ER PT J AU Okubara, PA Harrison, LA Gatch, EW Vandemark, G Schroeder, KL du Toit, LJ AF Okubara, P. A. Harrison, L. A. Gatch, E. W. Vandemark, G. Schroeder, K. L. du Toit, L. J. TI Development and Evaluation of a TaqMan Real-Time PCR Assay for Fusarium oxysporum f. sp spinaciae SO PLANT DISEASE LA English DT Article ID GENETIC DIVERSITY; SP CICERIS; VEGETATIVE COMPATIBILITY; CLADOSPORIUM-VARIABILE; STEMPHYLIUM-BOTRYOSUM; ARBITRARY PRIMERS; RAPID DETECTION; WILT PATHOGEN; SOIL; DNA AB Fusarium oxysporum f. sp. spinaciae, causal agent of spinach Fusarium wilt, is an important soilborne pathogen in many areas of the world where spinach is grown. The pathogen is persistent in acid soils of maritime western Oregon and Washington, the only region of the United States suitable for commercial spinach seed production. A TaqMan real-time polymerase chain reaction (PCR) assay was developed for rapid identification and quantification of the pathogen, based on sequencing the intergenic spacer (IGS) region of rDNA of isolates of the pathogen. A guanine single-nucleotide polymorphism (G SNP) was detected in the IGS sequences of 36 geographically diverse isolates of E oxysporum f. sp. spinaciae but not in the sequences of 64 isolates representing other formae speciales and 33 isolates representing other fungal species or genera. The SNP was used to develop a probe for a real-time PCR assay. The real-time PCR assay detected E oxysporum f. sp. spinaciae at 3-14,056 CFU/g of soil in 82 soil samples collected over 3 years from naturally infested spinach seed production sites in western Washington, although a reliable detection limit of the assay was determined to be 11 CFU/g of soil. A significant (P < 0.05), positive correlation between enumeration of F. oxysporum on Komada's agar and quantification of the pathogen using the TaqMan assay was observed in a comparison of 82 soil samples. Correlations between pathogen DNA levels, Fusarium wilt severity ratings, and spinach biomass were significantly positive for one set of naturally infested soils but not between pathogen DNA levels, wilt incidence ratings, and spinach biomass for other soil samples, suggesting that soilborne pathogen population is not the sole determinant of spinach Fusarium wilt incidence or severity. The presence of the G SNP detected in one isolate of each of E oxysporum ff. spp. lageneriae, lilii, melongenae, and raphani and reaction of the real-time PCR assay with 16 of 22 nonpathogenic isolates of E oxysporum associated with spinach plants or soil in which spinach had been grown potentially limits the application of this assay. Nonetheless, because all isolates of E oxysporum f. sp. spinaciae tested positive with the real-time PCR assay, the assay may provide a valuable means of screening for resistance to Fusarium wilt by quantifying development of the pathogen in spinach plants inoculated with the pathogen. C1 [Harrison, L. A.; Gatch, E. W.; du Toit, L. J.] Washington State Univ Mt Vernon NWREC, Mt Vernon, WA 98273 USA. [Okubara, P. A.] ARS, USDA, Root Dis & Biol Control Res Unit, Pullman, WA 99164 USA. [Vandemark, G.] ARS, USDA, Grain Legume Genet & Physiol Res Unit, Pullman, WA 99164 USA. [Schroeder, K. L.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. RP du Toit, LJ (reprint author), Washington State Univ Mt Vernon NWREC, Mt Vernon, WA 98273 USA. EM dutoit@wsu.edu FU United States Department of Agriculture (USDA) Agricultural Research Service [5248-22000-012-00D]; USDA Sustainable Agriculture Research and Education Graduate Student Fellow Grant; USDA Western Region Integrated Pest Management Grant; CRIS [WPN05595]; Washington State University CAHNRS, Agricultural Research Center; Department of Plant Pathology (PPNS) [0563] FX This research was supported by the United States Department of Agriculture (USDA) Agricultural Research Service Project Number 5248-22000-012-00D (P. A. Okubara and K. L. Schroeder), a USDA Sustainable Agriculture Research and Education Graduate Student Fellow Grant, a USDA Western Region Integrated Pest Management Grant, CRIS Project WPN05595, and Washington State University CAHNRS, Agricultural Research Center, and Department of Plant Pathology (PPNS number 0563). We thank T. Gordon, M. Kawabe, and J. Leslie for valuable discussions; J. Correll, B. Pryor, L. Hanson, M. Kawabe, and other cooperators for fungal isolates or DNA extracts of fungal isolates; L. Canis, X. Chen, T. Friesen, N. Grunwald, D. Johnson, M. Mazzola, T. Murray, R. Smiley, and D. Weller for providing some non-Fusarium DNA; M. Derie, K. Coats, and G. Chastagner for providing TaqMan data on isolates from Fiely et al. (21); N. Walter, L. Hulbert, and K. Reed for assistance with sequencing and DNA extractions; and K. Reed, B. Holmes, and L. Brissey for assistance with pathogenicity testing. NR 62 TC 6 Z9 6 U1 1 U2 32 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 927 EP 937 DI 10.1094/PDIS-03-12-0317-RE PG 11 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800009 ER PT J AU Keith, LM Matsumoto, TK Zee, FTP AF Keith, L. M. Matsumoto, T. K. Zee, F. T. P. TI First Report of Calonectria Leaf Spot on Ohelo in Hawaii. SO PLANT DISEASE LA English DT News Item C1 [Keith, L. M.; Matsumoto, T. K.; Zee, F. T. P.] ARS, Trop Plant Genet Resource & Dis Res Unit, Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. RP Keith, LM (reprint author), ARS, Trop Plant Genet Resource & Dis Res Unit, Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 990 EP 991 DI 10.1094/PDIS-09-12-0887-PDN PG 2 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800023 ER PT J AU Keith, LM Matsumoto, TK McQuate, GT AF Keith, L. M. Matsumoto, T. K. McQuate, G. T. TI First Report of Dolabra nepheliae Associated with Corky Bark Disease of Langsat in Hawaii. SO PLANT DISEASE LA English DT News Item C1 [Keith, L. M.; Matsumoto, T. K.] ARS, Trop Plant Genet Resource & Dis Res Unit, Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. [McQuate, G. T.] ARS, Trop Crop & Commod Protect Res Unit, Pacific Basin Agr Res Ctr, USDA, Hilo, HI USA. RP Keith, LM (reprint author), ARS, Trop Plant Genet Resource & Dis Res Unit, Pacific Basin Agr Res Ctr, USDA, Hilo, HI 96720 USA. NR 1 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 990 EP 990 DI 10.1094/PDIS-09-12-0886-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800022 ER PT J AU Jurick, WM Vico, I Gaskins, VL Janisiewicz, WJ Peter, KA AF Jurick, W. M., II Vico, I. Gaskins, V. L. Janisiewicz, W. J. Peter, K. A. TI First Report of Neofusicoccum ribis Causing Postharvest Decay of Apple Fruit from Cold Storage in Pennsylvania SO PLANT DISEASE LA English DT News Item C1 [Jurick, W. M., II; Gaskins, V. L.] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. [Vico, I.] Univ Belgrade, Dept Phytomed, Belgrade 11001, Serbia. [Janisiewicz, W. J.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Peter, K. A.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, Fruit Res & Educ Ctr, Biglerville, PA USA. RP Jurick, WM (reprint author), USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. NR 1 TC 0 Z9 0 U1 1 U2 6 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 999 EP 999 DI 10.1094/PDIS-01-13-0054-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800045 ER PT J AU Jurick, WM Vico, I Gaskins, VL Janisiewicz, WJ Peter, KA AF Jurick, W. M., II Vico, I. Gaskins, V. L. Janisiewicz, W. J. Peter, K. A. TI First Report of Botryosphaeria dothidea Causing White Rot on Apple Fruit in Maryland SO PLANT DISEASE LA English DT News Item C1 [Jurick, W. M., II; Gaskins, V. L.] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. [Vico, I.] Univ Belgrade, Dept Phytomed, Belgrade 11001, Serbia. [Janisiewicz, W. J.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Peter, K. A.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, Fruit Res & Educ Ctr, Biglerville, PA USA. RP Jurick, WM (reprint author), USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. NR 2 TC 1 Z9 1 U1 0 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 999 EP 999 DI 10.1094/PDIS-01-13-0053-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800044 ER PT J AU Rebbeck, J Malone, MA Short, DPG Kasson, MT O'Neal, ES Davis, DD AF Rebbeck, J. Malone, M. A. Short, D. P. G. Kasson, M. T. O'Neal, E. S. Davis, D. D. TI First Report of Verticillium Wilt Caused by Verticillium nonalfalfae on Tree-of-Heaven (Ailanthus altissima) in Ohio SO PLANT DISEASE LA English DT News Item C1 [Rebbeck, J.] US Forest Serv, USDA, Delaware, OH 43015 USA. [Malone, M. A.] F&W Forestry Serv, Chillicothe, OH USA. [Short, D. P. G.] Univ Calif Davis, Dept Plant Pathol, Salinas, CA USA. [Kasson, M. T.] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA USA. [O'Neal, E. S.; Davis, D. D.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Rebbeck, J (reprint author), US Forest Serv, USDA, Delaware, OH 43015 USA. NR 1 TC 4 Z9 5 U1 0 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 999 EP 1000 DI 10.1094/PDIS-01-13-0062-PDN PG 2 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800046 ER PT J AU Quito-Avila, DF Ibarra, MA Alvarez, RA Espinoza, L Ratti, MF Peralta, EL Martin, RR AF Quito-Avila, D. F. Ibarra, M. A. Alvarez, R. A. Espinoza, L. Ratti, M. F. Peralta, E. L. Martin, R. R. TI First Report of Raspberry bushy dwarf virus in Andean Blackberry (Rubus glaucus) in Central Ecuador SO PLANT DISEASE LA English DT News Item C1 [Quito-Avila, D. F.; Ibarra, M. A.; Alvarez, R. A.; Espinoza, L.; Ratti, M. F.; Peralta, E. L.] Escuela Super Politecn Litoral ESPOL, CIBE, Guayaquil, Ecuador. [Martin, R. R.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97331 USA. RP Quito-Avila, DF (reprint author), Escuela Super Politecn Litoral ESPOL, CIBE, Campus Gustavo Galindo Km 30-5 Via Perimetral,Apa, Guayaquil, Ecuador. NR 1 TC 0 Z9 0 U1 2 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 J9 PLANT DIS JI PLANT DIS. PD JUL PY 2013 VL 97 IS 7 BP 1003 EP 1003 DI 10.1094/PDIS-12-12-1198-PDN PG 1 WC Plant Sciences SC Plant Sciences GA 168AT UT WOS:000320678800055 ER PT J AU Lockhart, BR Gardiner, ES Leininger, TD Hamel, PB Connor, KF Devall, MS Schiff, NM Wilson, AD AF Lockhart, Brian Roy Gardiner, Emile S. Leininger, Theodor D. Hamel, Paul B. Connor, Kristina F. Devall, Margaret S. Schiff, Nathan M. Wilson, A. Dan TI Lindera melissifolia responses to flood durations and light regimes suggest strategies for recovery and conservation SO PLANT ECOLOGY LA English DT Article DE Active restoration; Gender; Lauraceae; Pondberry; Shade; Understory ID MISSISSIPPI; SOIL; AVAILABILITY; PONDBERRY; SEEDLINGS; GRADIENT; FORESTS; GROWTH; SHADE; BLUME AB Passive management to preserve endangered plant species involves measures to avoid anthropogenic disturbance of natural populations, but this approach may not sustain plants that require disturbance-maintained habitats. Active management is often necessary to maintain existing habitats or provide new habitats for endangered species recovery. Our objective was to examine the effects of two disturbances in floodplain forests, soil flooding and light availability, on survival, stem length, stem diameter and ramet production of endangered Lindera melissifolia (Walt.) Blume. We used a water impoundment facility to control the timing and duration of flooding (0, 45 or 90 days) and shade houses to vary light availability (70, 63 or 5 % ambient light). Hydroperiod had little direct effect on steckling survival, stem length growth and stem diameter growth, supporting indications that soil flooding may be important for reduction of interspecific competition in L. melissifolia habitat. Greater ramet production by stecklings receiving no soil flooding likely resulted from longer periods of favorable soil conditions during each growing season. Positive stem length growth and stem diameter growth under all light levels demonstrates the plasticity of this species to acclimate to a range of light environments, though, greatest survival and stem length growth occurred when L. melissifolia received 37 % light, and stem diameter growth was greatest beneath 70 % light. Further, female clones produced more ramets as light availability increased. These results indicate that passive management absent natural disturbance could jeopardize sustainability of extant L. melissifolia populations, and this species would respond favorably to active management practices that create canopy openings to increase understory light availability. C1 [Lockhart, Brian Roy; Gardiner, Emile S.; Leininger, Theodor D.; Hamel, Paul B.; Devall, Margaret S.; Schiff, Nathan M.; Wilson, A. Dan] US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, Stoneville, MS 38776 USA. [Connor, Kristina F.] US Forest Serv, USDA, Southern Res Stn, Auburn, AL 36849 USA. RP Lockhart, BR (reprint author), US Forest Serv, USDA, Southern Res Stn, Ctr Bottomland Hardwoods Res, POB 227, Stoneville, MS 38776 USA. EM blockhart@fs.fed.us RI Wilson, Alphus/Q-2137-2015 OI Wilson, Alphus/0000-0003-2352-5232 FU U.S. Army Corp of Engineers, Vicksburg District [SRS 01-IA-11330127-527]; U.S. Department of the Interior, Fish and Wildlife Service [SA0142-Amendment 3]; U.S. Department of Agriculture, Forest Service, Southern Research Station FX Support for this research was provided by the U.S. Army Corp of Engineers, Vicksburg District, Agreement Number SRS 01-IA-11330127-527; U.S. Department of the Interior, Fish and Wildlife Service under permit number Endangered-Threatened Species Subpermit SA0142-Amendment 3; and the U.S. Department of Agriculture, Forest Service, Southern Research Station. NR 28 TC 4 Z9 4 U1 0 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 J9 PLANT ECOL JI Plant Ecol. PD JUL PY 2013 VL 214 IS 7 BP 893 EP 905 DI 10.1007/s11258-013-0211-0 PG 13 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 174DU UT WOS:000321134600001 ER PT J AU Dai, J Bai, GH Zhang, DD Hong, DL AF Dai, Jian Bai, Guihua Zhang, Dadong Hong, Delin TI Validation of quantitative trait loci for aluminum tolerance in Chinese wheat landrace FSW SO EUPHYTICA LA English DT Article DE Chinese landrace; Aluminum tolerance; Simple sequence repeats; QTL mapping ID TRITICUM-AESTIVUM L.; CULTIVAR ATLAS-66; ACID SOILS; ALMT1 GENE; RESISTANCE; INHERITANCE; LOCATION; MARKERS; LINES; AL AB Aluminum (Al) toxicity is one of the major constraints for wheat production in acidic soils worldwide and use of Al-tolerant cultivars is one of the most effective approaches to reduce Al damage in the acidic soils. A Chinese landrace, FSW, shows a high level of tolerance to Al toxicity and a mapping population of recombinant inbred lines (RILs) was developed from a cross between FSW and Al-sensitive US spring wheat cultivar Wheaton to validate the quantitative trait loci (QTL) previously identified in FSW. The mapping population was evaluated for net root growth (NRG) during Al stress in a nutrient solution culture and hematoxylin staining score (HSS) of root tips after Al stress. After 132 simple sequence repeat (SSR) markers from three chromosomes that were previously reported to have the QTLs were analyzed in the population, two QTLs for Al tolerance from FSW were confirmed. The major QTL on chromosome 4DL co-segregated with the Al-activated malate transporter gene (ALMT1), however, sequence analysis of the promoter region (Ups4) of ALMT1 gene indicated that FSW contained a marker allele that is different from the one that was reported to condition Al tolerance in the Brazilian source. Another QTL on chromosome 3BL showed a minor effect on Al tolerance in the population. The two QTLs accounted for about 74.9 % of the phenotypic variation for HSS and 72.1 % for NRG and demonstrated an epistatic effect for both HSS and NRG. SSR markers closely linked to the QTLs have potential to be used for marker-assisted selection (MAS) to improve Al tolerance in wheat breeding programs. C1 [Dai, Jian] Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China. [Dai, Jian; Bai, Guihua; Zhang, Dadong] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Dai, Jian; Hong, Delin] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China. RP Bai, GH (reprint author), Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. EM gbai@ksu.edu FU National Research Initiative Competitive Grants CAP project from the USDA National Institute of Food and Agriculture [2011-68002-30029]; State Administration of Foreign Experts Affairs, China [CG2008320006] FX This project was partially supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and the scholarship to the first author from State Administration of Foreign Experts Affairs, China (no. CG2008320006). The authors would like to thank Dr. Paul St. Amand, USDA Central Small Grain Genotyping Center, and Dr. Chengsong Zhu, Department of Agronomy, Kansas State University, Manhattan KS, for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. This is contribution no. 12-413-J from the Kansas Agricultural Experiment Station, Manhattan, KS. NR 37 TC 3 Z9 3 U1 2 U2 33 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2013 VL 192 IS 2 BP 171 EP 179 DI 10.1007/s10681-012-0807-9 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 166SW UT WOS:000320578900002 ER PT J AU Yu, N Nachman, RJ Smagghe, G AF Yu, Na Nachman, Ronald J. Smagghe, Guy TI Characterization of sulfakinin and sulfakinin receptor and their roles in food intake in the red flour beetle Tribolium castaneum SO GENERAL AND COMPARATIVE ENDOCRINOLOGY LA English DT Article DE Sulfakinin; Sulfakinin receptor; Tribolium castaneum; Food intake; RNA interference ID BLOWFLY CALLIPHORA-VOMITORIA; SULFATED INSECT NEUROPEPTIDE; CRICKET GRYLLUS-BIMACULATUS; PEPTIDE ALPHA-AMIDATION; DROSOPHILA-MELANOGASTER; AMERICAN COCKROACH; SIGNAL PEPTIDES; SCHISTOCERCA-GREGARIA; NERVOUS-SYSTEM; FIELD CRICKET AB Sulfakinins (SK) are multifunctional neuropeptides widely found in insects that are structurally and functionally homologous to the mammalian gastrin/cholecystokinin (CCK) neuropeptides. CCK is involved in various biological processes such as the feeding regulation where it induces satiety. In this project we characterized SK and SK receptor (SKR) of an important pest and model beetle insect, the red flour beetle Tribolium castaneum, with the aim to better understand the SK signaling pathway and its function in food intake. The sk gene encoded a SK precursor with 113 amino acids and the skr gene a seven-transmembrane SKR with 554 amino acids. Both genes were expressed in the larval, pupal and adult stages with different expression levels in tested tissues. By RNA interference, sk dsRNA and skr dsRNA reduced the expression of the corresponding target gene by 80-90% and 30-50%, respectively, and stimulated food intake in the larvae. In parallel, we injected insects with a SK analog reducing food intake. In conclusion, the data are discussed in relation to the SK signaling pathway and its physiological-endocrinological role in regulating food intake and potential usage in the control of important pest insects. (C) 2013 Elsevier Inc. All rights reserved. C1 [Yu, Na; Smagghe, Guy] Univ Ghent, Dept Crop Protect, Fac Biosci Engn, B-9000 Ghent, Belgium. [Nachman, Ronald J.] Southern Plains Agr Res Ctr, Areawide Pest Management Res, USDA, College Stn, TX 77845 USA. RP Smagghe, G (reprint author), Univ Ghent, Dept Crop Protect, Fac Biosci Engn, B-9000 Ghent, Belgium. EM guy.smagghe@ugent.be FU China Scholar Council (CSC); Ghent University; U.S. Department of Agriculture FX The authors wish to acknowledge the financial support from the China Scholar Council (CSC) and the Special Research Fund of Ghent University, and the U.S. Department of Agriculture. NR 65 TC 20 Z9 20 U1 0 U2 22 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0016-6480 J9 GEN COMP ENDOCR JI Gen. Comp. Endocrinol. PD JUL 1 PY 2013 VL 188 BP 196 EP 203 DI 10.1016/j.ygcen.2013.03.006 PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA 168ZH UT WOS:000320746000027 PM 23524001 ER PT J AU Quiniou, SMA Boudinot, P Bengten, E AF Quiniou, Sylvie M. A. Boudinot, Pierre Bengten, Eva TI Comprehensive survey and genomic characterization of Toll-like receptors (TLRs) in channel catfish, Ictalurus punctatus: identification of novel fish TLRs SO IMMUNOGENETICS LA English DT Article DE Toll-like receptor; TLR25; TLR26; LRR; Channel catfish; Innate immunity ID LEUCINE-RICH REPEATS; GRASS CARP REOVIRUS; TROUT ONCORHYNCHUS-MYKISS; LARGE YELLOW CROAKER; DOUBLE-STRANDED-RNA; INDIAN MAJOR CARP; EXPRESSION ANALYSIS; MOLECULAR-CLONING; RAINBOW-TROUT; PARALICHTHYS-OLIVACEUS AB A comprehensive survey of channel catfish Toll-like receptors (TLRs) was undertaken following a genomic PCR approach based on degenerate primers. Twenty different TLRs were identified in channel catfish. Channel catfish TLR sequences were characterized by phylogenetic analysis based on their conserved Toll/interleukin-1 receptor domain and by in-depth analysis of leucine-rich repeat (LRR) motifs of the ligand binding extracellular domain (ECD). The catfish have representatives of all the TLR types defined in vertebrates with the exception of TLR6, TLR10, TLR11, TLR12, TLR13, TLR15, TLR23, and TLR24. Additionally, two new types were discovered: TLR25 and TLR26. TLR25 is also present in cyprinids, cichlids, plecoglossids, and adrianichthyids, suggesting its presence early in fish evolution. To date, TLR26 was found only in channel catfish. Like TLR18-23, TLR25 and TLR26 were not found in any other vertebrate classes and appear to be fish specific. Data mining using the catfish TLR sequences revealed that in addition to ictalurids and cyprinids, TLR4 is also present in salmonids. TLR19 and TLR20 were both found in ictalurids, cyprinids, and salmonids, demonstrating a wider range than previously known. The LRR structure within ECDs appeared generally well conserved. TLR7 demonstrated a very high identity to human TLR7 strongly suggesting that ligand specificity maybe conserved. Finally, expression profiling confirmed that most TLRs are widely expressed in a diversity of tissues and revealed marked differences of expression level. C1 [Quiniou, Sylvie M. A.] ARS, Catfish Genet Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. [Boudinot, Pierre] INRA, Domaine Vilvert, Mol Virol & Immunol, F-78350 Jouy En Josas, France. [Bengten, Eva] Univ Mississippi, Med Ctr, Dept Microbiol, Jackson, MS 39216 USA. RP Quiniou, SMA (reprint author), ARS, Catfish Genet Res Unit, USDA, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM sylvie.quiniou@ars.usda.gov RI boudinot, pierre/L-4246-2016 NR 119 TC 35 Z9 37 U1 5 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0093-7711 EI 1432-1211 J9 IMMUNOGENETICS JI Immunogenetics PD JUL PY 2013 VL 65 IS 7 BP 511 EP 530 DI 10.1007/s00251-013-0694-9 PG 20 WC Genetics & Heredity; Immunology SC Genetics & Heredity; Immunology GA 164FC UT WOS:000320393400004 PM 23558557 ER PT J AU Benjamin, L Atwill, ER Jay-Russell, M Cooley, M Carychao, D Gorski, L Mandrell, RE AF Benjamin, Lisa Atwill, Edward R. Jay-Russell, Michele Cooley, Michael Carychao, Diana Gorski, Lisa Mandrell, Robert E. TI Occurrence of generic Escherichia coli, E. coli O157 and Salmonella spp. in water and sediment from leafy green produce farms and streams on the Central California coast SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Water; Produce; Generic E. coli; E. coli O157; Salmonella spp.; Risk factors ID IRRIGATION WATER; DRINKING-WATER; SURFACE WATERS; FRESH PRODUCE; PATHOGENIC BACTERIA; INDICATOR BACTERIA; CHLORINE TREATMENT; WASTE-WATER; FOOD CROPS; SURVIVAL AB Irrigation with water of poor microbiological quality can elevate levels of bacteria on produce. This study aimed to identify climate and management variables associated with generic Escherichia coli in irrigation water on leafy green produce farms and to measure the prevalence of E. colt 0157 and Salmonella spp. in irrigation and non-irrigation water sources on these farms. Water and sediment samples collected from various points along irrigation systems, as well as from streams and ponds on farms on the Central California coast between May 27th, 2008 and October 26th, 2010 were cultured for generic E. colt (MPN/100 mL or cfu 100 g) (n = 436), E. coli 0157 (n = 437), and (n = 163) Salmonella. Variables were based on grower's management practices, landscape features in proximity to samples (e.g., distance to roads and ranches/livestock), and climate data accessed from an online database. Negative binomial regression models were constructed to test associations between generic E. coli (MPN/100 mL) in water from farms and variables. Arithmetic mean concentration of E. coli for water, not including those from Moore swabs, and sediment samples, was 7.1 x 10(2) MPN/100 mL and 1.0 x 10(4) cfu/100 g, respectively. Matched by collection day, E. coli concentration in sediment (cfu/100 g) was typically 10- to 1000-fold higher than the overlying water (MPN/100 mL) for these irrigation systems. Generic E. coli concentration (MPN/100 mL) increased by 60.1% for each 1 m/s increase in wind speed and decreased by 3% for each 10 m increase in the distance between the sample location and rangeland. Moore swabs detected a higher proportion of E. coli 0157 (13.8%) positive water samples compared to grab samples (1.8%); 1.7% of sediment samples had detectable levels of this pathogen. Interestingly, season was not significantly associated with E. coli 0157 presence in water or sediments from produce farms or water sources with public access. Salmonella was detected in 6% (6/96) water and 43% (3/67) sediment samples. Generic E. coli concentration was not significantly associated with the presence of either E. coli 0157 or Salmonella in water or sediment samples, suggesting that, for this 2.5-year period and geographical location, generic E. coli would likely be an unreliable indicator bacteria for predicting the presence of these food- and waterborne pathogens in a key produce production environment. (C) 2013 Elsevier B.V. All rights reserved. C1 [Benjamin, Lisa; Atwill, Edward R.; Jay-Russell, Michele] Univ Calif Davis, Western Ctr Food Safety, Davis, CA 95618 USA. [Cooley, Michael; Carychao, Diana; Gorski, Lisa; Mandrell, Robert E.] USDA ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. RP Atwill, ER (reprint author), Univ Calif Davis, Western Ctr Food Safety, 1477 Drew Ave,Ste 101, Davis, CA 95618 USA. EM ratwill@wifss.ucdavis.edu FU U.S. Food and Drug Administration [U01-003-572]; National Research Initiative Competitive Grant from U.S. Department of Agriculture National Institute of Food and Agriculture [2006-55212-16927, 2007-35212-18239]; U.S. Department of Agriculture, Agricultural Research Service, CRIS [5325-42000-044-00D]; Center for Food Safety and Applied Nutrition FX This research was funded by the U.S. Food and Drug Administration Project No. U01-003-572; by National Research Initiative Competitive Grant nos. 2006-55212-16927 and 2007-35212-18239 from the U.S. Department of Agriculture National Institute of Food and Agriculture; and the U.S. Department of Agriculture, Agricultural Research Service, CRIS project number 5325-42000-044-00D. Also, this project was supported in part by an appointment to the Research Participation at the Center for Food Safety and Applied Nutrition administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Food and Drug Administration. NR 85 TC 35 Z9 35 U1 6 U2 79 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JUL 1 PY 2013 VL 165 IS 1 BP 65 EP 76 DI 10.1016/j.ijfoodmicro.2013.04.003 PG 12 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA 167MA UT WOS:000320634900010 PM 23697918 ER PT J AU Ha, W Gowda, PH Howell, TA AF Ha, Wonsook Gowda, Prasanna H. Howell, Terry A. TI A review of downscaling methods for remote sensing-based irrigation management: part I SO IRRIGATION SCIENCE LA English DT Review ID SUPPORT VECTOR MACHINES; SCALE-RECURSIVE ESTIMATION; SPECTRAL MIXTURE ANALYSIS; LAND-SURFACE TEMPERATURE; ENERGY BALANCE ALGORITHM; THEMATIC MAPPER IMAGERY; NEURAL-NETWORKS; SOIL-MOISTURE; CLIMATE-CHANGE; MAPPING EVAPOTRANSPIRATION AB High-resolution daily evapotranspiration (ET) maps would greatly improve irrigation management. Numerous ET mapping algorithms have been developed to make use of thermal remote sensing data acquired by satellite sensors. However, adoption of remote sensing-based ET maps for irrigation management has not been feasible due to inadequate spatial and temporal resolution of ET maps. Data from a coarse spatial resolution image in agricultural fields often cause inaccurate ET estimation because of a high level of spatial heterogeneity in land use. Image downscaling methods have been utilized to overcome spatial and temporal scaling issues in numerous remote sensing applications. In the field of hydrology, the image downscaling method has been used to improve spatial resolution of remote sensing-based ET maps for irrigation scheduling purposes and thus improves estimation of crop water requirements. This paper (part I) reviews downscaling methods to improve spatial resolution of land surface characteristics such as land surface temperature or ET. Each downscaling method was assessed and compared with respect to their capability of downscaling spatial resolutions of images. The companion paper (part II) presents review of image fusion methods that are also designed to increase spatial resolutions of images by integrating multi-spectral and panchromatic images. C1 [Ha, Wonsook; Gowda, Prasanna H.; Howell, Terry A.] ARS, Conservat & Prod Res Lab, USDA, Bushland, TX 79012 USA. RP Ha, W (reprint author), ARS, Conservat & Prod Res Lab, USDA, PO Drawer 10, Bushland, TX 79012 USA. EM Wonsook.Ha@gmail.com FU USDA-ARS; NASA Terrestrial Hydrology Program [08-THP07-0053] FX Funding for this study was provided by USDA-ARS and NASA Terrestrial Hydrology Program (Proposal No. 08-THP07-0053). Authors are grateful to reviewers who provided valuable comments. NR 110 TC 13 Z9 15 U1 8 U2 80 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 EI 1432-1319 J9 IRRIGATION SCI JI Irrig. Sci. PD JUL PY 2013 VL 31 IS 4 BP 831 EP 850 DI 10.1007/s00271-012-0331-7 PG 20 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 164CR UT WOS:000320386900025 ER PT J AU Ha, W Gowda, PH Howell, TA AF Ha, Wonsook Gowda, Prasanna H. Howell, Terry A. TI A review of potential image fusion methods for remote sensing-based irrigation management: part II SO IRRIGATION SCIENCE LA English DT Review ID SPECTRAL RESOLUTION IMAGES; LANDSAT THEMATIC MAPPER; SPOT PANCHROMATIC DATA; BAYESIAN DATA FUSION; WAVELET TRANSFORM; CURVELET TRANSFORM; MULTIRESOLUTION ANALYSIS; ARSIS CONCEPT; CONTOURLET TRANSFORM; MULTISPECTRAL IMAGES AB Satellite-based sensors provide data at either greater spectral and coarser spatial resolutions or lower spectral and finer spatial resolutions due to complementary spectral and spatial characteristics of optical sensor systems. In order to overcome this limitation, image fusion has been suggested to obtain higher spatial and spectral resolution images at the same time. Image fusion has been a valuable technique in digital image analysis and comparison because of the availability of multi-spatial and multispectral images from satellite and airborne sensors. It has been applied to merge coarser spatial resolution of multispectral images with a finer spatial resolution panchromatic image to enhance visual apprehension and to provide images that are more informative. Part I companion paper presented and discussed the image downscaling methods. In this paper (part II), the main objective is to review existing image fusion methods for their capability to downscale coarser spatial resolution images for irrigation management applications. A literature review indicated that image fusion methods have not been actively used in obtaining high-resolution land surface temperature (LST) and evapotranspiration (ET) images for irrigation management. However, there is a great potential for applying image fusion methods to retrieve finer LST and ET images from coarser thermal images by fusing them with finer non-thermal color or panchromatic images for irrigation scheduling and management purposes. C1 [Ha, Wonsook; Gowda, Prasanna H.; Howell, Terry A.] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. RP Ha, W (reprint author), USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. EM Wonsook.Ha@gmail.com FU USDA-ARS; NASA Terrestrial Hydrology Program [08-THP07-0053] FX Funding for this study was provided by USDA-ARS and NASA Terrestrial Hydrology Program (Proposal No. 08-THP07-0053). Authors are grateful to reviewers who provided valuable comments. NR 81 TC 7 Z9 8 U1 7 U2 45 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 J9 IRRIGATION SCI JI Irrig. Sci. PD JUL PY 2013 VL 31 IS 4 BP 851 EP 869 DI 10.1007/s00271-012-0340-6 PG 19 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 164CR UT WOS:000320386900026 ER PT J AU Evans, RG LaRue, J Stone, KC King, BA AF Evans, Robert G. LaRue, Jake Stone, Kenneth C. King, Bradley A. TI Adoption of site-specific variable rate sprinkler irrigation systems SO IRRIGATION SCIENCE LA English DT Review ID WIRELESS SENSOR NETWORK; CROP CANOPY TEMPERATURE; DEFICIT IRRIGATION; PRECISION AGRICULTURE; WATER PRODUCTIVITY; MANAGEMENT; AUTOMATION AB More than 20 years of private and public research on site-specific variable rate sprinkler irrigation (SS-VRI) technology on self-propelled center pivot and linear move irrigation systems has resulted in limited commercial adoption of the technology. Competing patents, liability, and proprietary software have affected industry's willingness to move into a new technology area. Documented and proven water conservation strategies using site-specific irrigation are quite limited. Marginal costs associated with site-specific technologies are high. Although sales of SS-VRI are increasing, they are primarily being used for eliminating irrigation and chemigation on non-cropped areas of a field or for land application of liquid agricultural and municipal wastes. Various aspects of SS-VRI technologies for general crop production are beginning to slowly gain widespread acceptance; however, their uses are largely focused on addressing symptoms of poor design and sub-optimal water and nutrient management. Although currently underutilized, SS-VRI technology has the potential to positively impact crop water productivity, water and energy conservation, and the environment. There are also few economic incentives to motivate growers to move to higher levels of SS-VRI management. Greater adoption rates will likely require higher costs for water and energy, severely restricted water diversions on a broad scale, and enforcement of compliance with environmental and other regulations. Sustainable use of SS-VRI will require strong research support, which is currently limited. In the short term, adoption of SS-VRI technologies will be enhanced by addressing equipment deficiencies and research developing basic criteria and systems for defining management zones and locations of various sensor systems for both arid and humid regions. Training adequate personnel to help write site-specific variable rate irrigation prescriptions in humid and arid areas to assist growers with the decision-making process is also a high priority. There is also a large need to educate government boards and bankers on the potential benefits of these systems. The long-term challenges will be to demonstrate that SS-VRI will improve water management or increase net returns. There is a critical need to develop fully integrated management systems with supporting elements that accurately and inexpensively define dynamic management zones, sense within-field variability in real time, and then adaptively control site-specific variable rate water applications, which will be challenging as significant knowledge gaps exist. C1 [Evans, Robert G.] ARS, USDA, Sidney, MT 59270 USA. [LaRue, Jake] Valmont Ind Inc, Applicat & Project Support, Valley, NE 68064 USA. [Stone, Kenneth C.] ARS, USDA, Florence, SC 29501 USA. [King, Bradley A.] ARS, USDA, Kimberly, ID 83341 USA. RP Evans, RG (reprint author), ARS, USDA, 1500 N Cent Ave, Sidney, MT 59270 USA. EM Robert.Evans@ars.usda.gov; jlarue@valmont.com; Ken.Stone@ars.usda.gov; Brad.King@ars.usda.gov NR 48 TC 15 Z9 16 U1 5 U2 74 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0342-7188 J9 IRRIGATION SCI JI Irrig. Sci. PD JUL PY 2013 VL 31 IS 4 BP 871 EP 887 DI 10.1007/s00271-012-0365-x PG 17 WC Agronomy; Water Resources SC Agriculture; Water Resources GA 164CR UT WOS:000320386900027 ER PT J AU Valles, SM Porter, SD Choi, MY Oi, DH AF Valles, Steven M. Porter, Sanford D. Choi, Man-Yeon Oi, David H. TI Successful transmission of Solenopsis invicta virus 3 to Solenopsis invicta fire ant colonies in oil, sugar, and cricket bait formulations SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Biopesticide; Microbial control; Fire ant virus; Picornavirales; Single-stranded RNA virus; Formicidae ID C-VIRUS; RNA; POPULATIONS; SACBROOD; PCR; BEE AB Tests were conducted to evaluate whether Solenopsis invicta virus 3 (SINV-3) could be delivered in various bait formulations to fire ant colonies and measure the corresponding colony health changes associated with virus infection in Solenopsis invicta. Three bait formulations (10% sugar solution, cricket paste, and soybean oil adsorbed to defatted corn grit) effectively transmitted SINV-3 infections to S. invicta colonies. Correspondingly, viral infection was shown to be detrimental to colony health and productivity. By day 32, all ant colonies exposed to a single 24 h pulse treatment of SINV-3 became infected with the virus regardless of the bait formulation. However, the SINV-3 sugar and cricket bait-treated colonies became infected more rapidly than the oil-treated colonies. Sugar and cricket-treated colonies exhibited significant declines in their brood ratings compared with the untreated control and oil bait-treated colonies. Measures of colony health and productivity evaluated at the end of the study (day 47) showed a number of differences among the bait treatments and the control group. Statistically significant and similar patterns were exhibited among treatments for the quantity of live workers (lower), live brood (lower), total colony weight (lower), worker mortality (higher), proportion larvae (lower), and queen weight (lower). Significant changes were also observed in the number of eggs laid by queens (lower) and the corresponding ovary rating in SINV-3-treated colonies. The study provides the first successful demonstration of SINV-3 as a potential biopesticide against fire ants. Published by Elsevier Inc. C1 [Valles, Steven M.; Porter, Sanford D.; Choi, Man-Yeon; Oi, David H.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. RP Valles, SM (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov OI Choi, Man-Yeon/0000-0003-0769-380X NR 29 TC 13 Z9 13 U1 1 U2 25 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUL PY 2013 VL 113 IS 3 BP 198 EP 204 DI 10.1016/j.jip.2013.04.003 PG 7 WC Zoology SC Zoology GA 167LQ UT WOS:000320633900002 PM 23602901 ER PT J AU Valles, SM Oi, DH Plowes, RM Sanchez-Arroyo, H Varone, L Conant, P Webb, G AF Valles, Steven M. Oi, David H. Plowes, Robert M. Sanchez-Arroyo, Hussein Varone, Laura Conant, Pat Webb, Garry TI Geographic distribution suggests that Solenopsis invicta is the host of predilection for Solenopsis invicta virus 1 SO JOURNAL OF INVERTEBRATE PATHOLOGY LA English DT Article DE Solenopsis invicta virus; Fire ant; Sympatric; Allopatric; Pathogen spillover; Pathogen pollution ID IMPORTED FIRE ANT; HYMENOPTERA-FORMICIDAE; DISCOVERY; SEQUENCE AB Solenopsis invicta virus 1 (SINV-1) was found regularly and prevalently in S. invicta. In sampled locations where S. invicta and S. geminata are sympatric (specifically, Gainesville, FL and Travis, TX), SINV-1 was detected in S. geminata. Conversely, in areas in which S. geminata and S. invicta are allopatric, SINV-1 was not detected in S. geminata; these locations included north Australia (n = 12), southern Mexico (n = 107), Hawaii (n = 48), Taiwan (n = 12), and the Johnston Atoll (n = 6). A similar relationship was observed for S. richteri. In areas in which S. invicta and S. richteri were sympatric, SINV-1 was detected in the S. richteri population, but in areas in which S. invicta and S. richteri were allopatric, SINV-1 was not detected. These occurrences suggest that S. invicta is the host of predilection, or preferred host for SINV-1, and that the congenerics, S. geminata and S. richteri serve as either accidental, reservoir, or transfer hosts. The minus genome strand of SINV-1 was detected in S. geminata and S. richteri indicating that these species may serve as functional hosts capable of supporting SINV-1 replication. SINV-1 was not detected in S. xyloni regardless of its proximity to S. invicta. These results suggest that SINV-1 may be an example of pathogen spillover or pollution. Published by Elsevier Inc. C1 [Valles, Steven M.; Oi, David H.] USDA ARS, Imported Fire Ant & Household Insects Res Unit, Gainesville, FL 32608 USA. [Plowes, Robert M.] Univ Texas Austin, Sect Integrat Biol, Brackenridge Field Lab, Austin, TX 78712 USA. [Sanchez-Arroyo, Hussein] Inst Fitosanidad, Mexico City 56230, DF, Mexico. [Varone, Laura] Fdn Estudio Especies Invasivas, Buenos Aires, DF, Argentina. [Conant, Pat] Hawaii Dept Agr, Hilo, HI USA. [Webb, Garry] Sumitomo Chem Australia, Epping, NSW 2121, Australia. RP Valles, SM (reprint author), USDA ARS, Imported Fire Ant & Household Insects Res Unit, 1600 SW 23rd Dr, Gainesville, FL 32608 USA. EM steven.valles@ars.usda.gov NR 27 TC 1 Z9 1 U1 3 U2 17 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0022-2011 EI 1096-0805 J9 J INVERTEBR PATHOL JI J. Invertebr. Pathol. PD JUL PY 2013 VL 113 IS 3 BP 232 EP 236 DI 10.1016/j.jip.2013.04.006 PG 5 WC Zoology SC Zoology GA 167LQ UT WOS:000320633900008 PM 23623900 ER PT J AU Feng, ZX Gomez, J Bowman, AS Ye, JQ Long, LP Nelson, SW Yang, JL Martin, B Jia, K Nolting, JM Cunningham, F Cardona, C Zhang, JQ Yoon, KJ Slemons, RD Wan, XF AF Feng, Zhixin Gomez, Janet Bowman, Andrew S. Ye, Jianqiang Long, Li-Ping Nelson, Sarah W. Yang, Jialiang Martin, Brigitte Jia, Kun Nolting, Jacqueline M. Cunningham, Fred Cardona, Carol Zhang, Jianqiang Yoon, Kyoung-Jin Slemons, Richard D. Wan, Xiu-Feng TI Antigenic Characterization of H3N2 Influenza A Viruses from Ohio Agricultural Fairs SO JOURNAL OF VIROLOGY LA English DT Article ID SWINE INFLUENZA; UNITED-STATES; ANTIBODY-RESPONSES; GENETIC EVOLUTION; HIGH-THROUGHPUT; AMERICAN PIGS; INFECTION; HEMAGGLUTININ; HUMANS; TRANSMISSION AB The demonstrated link between the emergence of H3N2 variant (H3N2v) influenza A viruses (IAVs) and swine exposure at agricultural fairs has raised concerns about the human health risk posed by IAV-infected swine. Understanding the antigenic profiles of IAVs circulating in pigs at agricultural fairs is critical to developing effective prevention and control strategies. Here, 68 H3N2 IAV isolates recovered from pigs at Ohio fairs (2009 to 2011) were antigenically characterized. These isolates were compared with other H3 IAVs recovered from commercial swine, wild birds, and canines, along with human seasonal and variant H3N2 IAVs. Antigenic cartography demonstrated that H3N2 IAV isolates from Ohio fairs could be divided into two antigenic groups: (i) the 2009 fair isolates and (ii) the 2010 and 2011 fair isolates. These same two antigenic clusters have also been observed in commercial swine populations in recent years. Human H3N2v isolates from 2010 and 2011 are antigenically clustered with swine-origin IAVs from the same time period. The isolates recovered from pigs at fairs did not cross-react with ferret antisera produced against the human seasonal H3N2 IAVs circulating during the past decade, raising the question of the degree of immunity that the human population has to swine-origin H3N2 IAVs. Our results demonstrate that H3N2 IAVs infecting pigs at fairs and H3N2v isolates were antigenically similar to the IAVs circulating in commercial swine, demonstrating that exhibition swine can function as a bridge between commercial swine and the human population. C1 [Feng, Zhixin; Gomez, Janet; Ye, Jianqiang; Long, Li-Ping; Yang, Jialiang; Martin, Brigitte; Jia, Kun; Wan, Xiu-Feng] Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. [Feng, Zhixin] Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China. [Bowman, Andrew S.; Nelson, Sarah W.; Nolting, Jacqueline M.; Slemons, Richard D.] Ohio State Univ, Coll Vet Med, Dept Vet Prevent Med, Columbus, OH 43210 USA. [Cunningham, Fred] Natl Wildlife Res Ctr, Mississippi Field Stn, USDA APHIS WS, Mississippi State, MS USA. [Cardona, Carol] Univ Minnesota, Coll Vet Med, St Paul, MN 55108 USA. [Zhang, Jianqiang; Yoon, Kyoung-Jin] Iowa State Univ, Coll Vet Med, Dept Vet Diagnost & Prod Anim Med, Ames, IA USA. RP Wan, XF (reprint author), Mississippi State Univ, Coll Vet Med, Dept Basic Sci, Mississippi State, MS 39762 USA. EM wan@cvm.msstate.edu RI Bowman, Andrew/B-4321-2012; Nolting, Jacqueline/C-4464-2015; OI Bowman, Andrew/0000-0002-0738-8453; Yang, Jialiang/0000-0003-4689-8672 FU NIH [5T35RR007071, RC1AI086830]; Minnesota Center of Excellence for Influenza Research and Surveillance (MCEIRS); Centers of Excellence for Influenza Research and Surveillance (CEIRS); National Institute of Allergy and Infectious Diseases; National Institutes of Health; Department of Health and Human Services [HHSN266200700007C] FX This study was supported by NIH grants 5T35RR007071 and RC1AI086830. Additionally, this work has been funded in part by the Minnesota Center of Excellence for Influenza Research and Surveillance (MCEIRS) with federal funds from the Centers of Excellence for Influenza Research and Surveillance (CEIRS), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under contract no. HHSN266200700007C. NR 51 TC 11 Z9 11 U1 0 U2 7 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X J9 J VIROL JI J. Virol. PD JUL PY 2013 VL 87 IS 13 BP 7655 EP 7667 DI 10.1128/JVI.00804-13 PG 13 WC Virology SC Virology GA 160JT UT WOS:000320116500039 PM 23637412 ER PT J AU Aubry, KB Raley, CM Buskirk, SW Zielinski, WJ Schwartz, MK Golightly, RT Purcell, KL Weir, RD Yaeger, JS AF Aubry, Keith B. Raley, Catherine M. Buskirk, Steven W. Zielinski, William J. Schwartz, Michael K. Golightly, Richard T. Purcell, Kathryn L. Weir, Richard D. Yaeger, J. Scott TI Meta-analyses of habitat selection by fishers at resting sites in the pacific coastal region SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE fisher; habitat selection; meta-analysis; Pacific coastal region; Pekania pennanti; resting site ID MARTES-PENNANTI; CALIFORNIA AB The fisher (Pekania pennanti) is a species of conservation concern throughout the Pacific coastal region in North America. A number of radiotelemetry studies of habitat selection by fishers at resting sites have been conducted in this region, but the applicability of observed patterns beyond the boundaries of each study area is unknown. Broadly applicable information on habitat selection by fishers in this region would be useful for conservation planning and for informing forest management decisions in areas where intensive field studies have not been conducted. To provide such information, we conducted formal meta-analyses of habitat selection by fishers at resting sites in 8 study areas located from central British Columbia to the southern Sierra Nevada in California, including all areas that currently contain established fisher populations. Each study included in the meta-analyses measured environmental attributes at sites used by fishers for resting (i.e., the immediate vicinity of resting structures; typically 0.5ha) and at random or systematically located sites representing resource availability in each study area. We selected 9 environmental attributes that we expected to be associated with fisher resting sites: slope, heat load index, percent cover of vegetation 2m above the ground, volume of moderately decayed logs 26cm in mean diameter, basal area of live conifers 51-100cm in diameter at breast height (dbh), basal area of live hardwoods 51-100cm in dbh, basal area of moderately decayed snags 51-100cm in dbh, mean dbh of live conifers 10cm in dbh, and mean dbh of live hardwoods 10cm in dbh. Despite substantial variation in environmental conditions among study locations, our analyses revealed statistically significant summary effect sizes for each of the 9 environmental attributes we analyzed. Fishers selected sites for resting that had steeper slopes, cooler microclimates, denser overhead cover, a greater volume of logs, and a greater prevalence of large trees and snags than were generally available. Thus, in areas within the Pacific coastal region where fishers have not been studied and data on selection of resting sites are lacking, our findings provide empirical support for management or conservation actions for fishers that promote the retention or development of these environmental attributes. (c) 2013 The Wildlife Society. C1 [Aubry, Keith B.; Raley, Catherine M.] US Forest Serv, Pacific NW Res Stn, USDA, Olympia, WA 98512 USA. [Buskirk, Steven W.] Univ Wyoming, Dept Zool & Physiol, Laramie, WY 82071 USA. [Zielinski, William J.] US Forest Serv, Pacific Southwest Res Stn, USDA, Arcata, CA 95521 USA. [Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59801 USA. [Golightly, Richard T.] Humboldt State Univ, Dept Wildlife, Arcata, CA 95521 USA. [Purcell, Kathryn L.] US Forest Serv, Pacif Southwest Res Stn, USDA, Fresno, CA 93710 USA. [Weir, Richard D.] Artemis Wildlife Consultants, Armstrong, BC V0E 1B4, Canada. [Yaeger, J. Scott] US Fish & Wildlife Serv, USDI, Yreka, CA 96097 USA. RP Aubry, KB (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM kaubry@fs.fed.us RI Schwartz, Michael/C-3184-2014; Purcell, Kathryn/S-2592-2016; Buskirk, Steven/K-2871-2016 OI Schwartz, Michael/0000-0003-3521-3367; Buskirk, Steven/0000-0003-0611-6648 FU Forest Service; Bureau of Land Management; Fish and Wildlife Service FX Financial support for this study was provided by the Forest Service, Bureau of Land Management, and Fish and Wildlife Service. R. Schlexer assisted in collating and organizing data for the Pilot Creek and Tule River studies. We are especially grateful to A. Steel of the Pacific Northwest Research Station for assistance with statistical analyses. NR 36 TC 15 Z9 15 U1 4 U2 43 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD JUL PY 2013 VL 77 IS 5 BP 965 EP 974 DI 10.1002/jwmg.563 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA 171NV UT WOS:000320937000011 ER PT J AU Fraser, JC Bazuin, JT Band, LE Grove, JM AF Fraser, James Curtis Bazuin, Joshua Theodore Band, Lawrence E. Grove, J. Morgan TI Covenants, cohesion, and community: The effects of neighborhood governance on lawn fertilization SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Lawn fertilization; Nitrogen application rate; Neighborhood governance; Homeowners associations; Social cohesion; Non-point source pollution ID OPEN SPACE; IMPUTATION; NITROGEN; VALUES AB Lawn fertilization is increasingly recognized as an important contributor to non-point source pollution in watersheds, but relatively little is known about how and why homeowners fertilize. Lawns are a social expression of citizenship and belonging in many American cities, for a well-maintained yard reflects a homeowner's work ethic as well as the pride in his home. There are also neighborhood influences, as homeowners conform to the dominant neighborhood standard of lawn esthetics. Homeowners associations (HOAs) are one way in which neighborhood lawn standards are maintained, as they use written rules and unwritten expectations backed by legal means of enforcement to ensure compliance with neighborhood guidelines. This paper examines household nitrogen fertilizer application rates in Baltimore, Maryland. We find that households which place a high importance on lawn care and occupy more valuable homes fertilize at higher rates compared with neighbors who place lower importance on lawn care, and live in less expensive homes. We also examine the effects of different neighborhood governance regimes, specifically homeowners associations and neighborhood associations. Households who belong to an HOA apply more fertilizer than those who do not, but households belonging to a neighborhood association do not fertilize more than their counterparts who are not so affiliated. HOA membership also mediates the effect of lawn care importance and home value and moderates the effect of social cohesion on fertilization application rates. HOAs shape household lawn behaviors: by obliging people to maintain a high esthetic standard, they encourage higher usage of chemicals to attain those standards. (C) 2013 Elsevier B.V. All rights reserved. C1 [Fraser, James Curtis] Vanderbilt Univ, Peabody Coll Educ & Human Dev, Dept Human & Org Dev, Nashville, TN 37203 USA. [Bazuin, Joshua Theodore] Vanderbilt Univ, Inst Energy & Environm, Nashville, TN 37203 USA. [Band, Lawrence E.] Univ N Carolina, Dept Geog, Chapel Hill, NC USA. [Band, Lawrence E.] Univ N Carolina, Inst Environm, Chapel Hill, NC 27515 USA. [Grove, J. Morgan] US Forest Serv, USDA, Baltimore Field Stn, Catonsville, MD 21228 USA. [Grove, J. Morgan] US Forest Serv, USDA, No Res Stn, Baltimore Ecosyst Study, Catonsville, MD 21228 USA. RP Fraser, JC (reprint author), Vanderbilt Univ, Peabody Coll Educ & Human Dev, Dept Human & Org Dev, GPC Box 90,230 Appleton Pl, Nashville, TN 37203 USA. EM james.c.fraser@vanderbilt.edu; joshua.tbazuin@vanderbilt.edu; lband@email.unc.edu; mgrove@fs.fed.us FU National Science Foundation [0729387] FX This research was supported by the Human and Social Dynamics Program of the National Science Foundation, grant number 0729387. NR 48 TC 16 Z9 16 U1 12 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD JUL PY 2013 VL 115 BP 30 EP 38 DI 10.1016/j.landurbplan.2013.02.013 PG 9 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA 172RW UT WOS:000321023600004 ER PT J AU Rua, MA Umbanhowar, J Hu, SJ Burkey, KO Mitchell, CE AF Rua, Megan A. Umbanhowar, James Hu, Shuijin Burkey, Kent O. Mitchell, Charles E. TI Elevated CO2 spurs reciprocal positive effects between a plant virus and an arbuscular mycorrhizal fungus SO NEW PHYTOLOGIST LA English DT Article DE arbuscular mycorrhizas; Avena fatua; Barley yellow dwarf virus; Bromus hordeaceus; carbon dioxide; microbes; phosphorus; plant-soil interactions ID BARLEY YELLOW DWARF; ATMOSPHERIC CO2; INFECTIOUS-DISEASE; CARBON-DIOXIDE; ANNUAL GRASSES; ACQUISITION; RESPONSES; PHOSPHORUS; NITROGEN; GROWTH AB Plants form ubiquitous associations with diverse microbes. These interactions range from parasitism to mutualism, depending partly on resource supplies that are being altered by global change. While many studies have considered the separate effects of pathogens and mutualists on their hosts, few studies have investigated interactions among microbial mutualists and pathogens in the context of global change. Using two wild grass species as model hosts, we grew individual plants under ambient or elevated CO2, and ambient or increased soil phosphorus (P) supply. Additionally, individuals were grown with or without arbuscular mycorrhizal inoculum, and after 2wk, plants were inoculated or mock-inoculated with a phloem-restricted virus. Under elevated CO2, mycorrhizal association increased the titer of virus infections, and virus infection reciprocally increased the colonization of roots by mycorrhizal fungi. Additionally, virus infection decreased plant allocation to root biomass, increased leaf P, and modulated effects of CO2 and P addition on mycorrhizal root colonization. These results indicate that plant mutualists and pathogens can alter each other's success, and predict that these interactions will respond to increased resource availability and elevated CO2. Together, our findings highlight the importance of interactions among multiple microorganisms for plant performance under global change. C1 [Rua, Megan A.; Umbanhowar, James; Mitchell, Charles E.] Univ N Carolina, Curriculum Environm & Ecol, Chapel Hill, NC 27599 USA. [Rua, Megan A.] Univ Mississippi, Dept Biol, Oxford, MS 38677 USA. [Umbanhowar, James; Mitchell, Charles E.] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA. [Hu, Shuijin] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Burkey, Kent O.] N Carolina State Univ, USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Burkey, Kent O.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Rua, MA (reprint author), Univ N Carolina, Curriculum Environm & Ecol, Chapel Hill, NC 27599 USA. EM marua@olemiss.edu RI Mitchell, Charles/I-3709-2014; OI Mitchell, Charles/0000-0002-1633-1993; Rua, Megan A./0000-0002-2883-2795 FU NSF-NIH Ecology of Infectious Disease program through NSF [EF-05-25641, DEB-10-15909]; NSF GRFP; National Science Foundation [DBI-12-02676] FX We are thankful to J. Barton at the CSTR facility for technical help. We would like to thank J. Bever and K. Vogelsang for mycorrhizal inoculum. We are grateful to the Borer-Seabloom lab for collecting and providing all plant seed, and to Jack Weiss for statistical advice. We would also like to thank the Mitchell lab for assistance. This research was partially supported by the joint NSF-NIH Ecology of Infectious Disease program through NSF Grants EF-05-25641 and DEB-10-15909 to C. E. M., an NSF GRFP to M. A. R., and the National Science Foundation Postdoctoral Research Fellowship in Biology under Grant no. DBI-12-02676 to M.A.R. NR 55 TC 13 Z9 14 U1 10 U2 98 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JUL PY 2013 VL 199 IS 2 BP 541 EP 549 DI 10.1111/nph.12273 PG 9 WC Plant Sciences SC Plant Sciences GA 167GC UT WOS:000320618400022 PM 23594373 ER PT J AU Hagerman, AD Ward, MP Anderson, DP Looney, JC McCarl, BA AF Hagerman, Amy D. Ward, Michael P. Anderson, David P. Looney, J. Chris McCarl, Bruce A. TI Rapid effective trace-back capability value: A case study of foot-and-mouth in the Texas High Plains SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Traceability; Market chain; Foot-and mouth disease; Veterinary epidemiology; Economics; Spread model ID DISEASE; SPREAD AB In this study our aim was to value the benefits of rapid effective trace-back capability-based on a livestock identification system - in the event of a foot and mouth disease (FMD) outbreak. We simulated an FMD outbreak in the Texas High Plains, an area of high livestock concentration, beginning in a large feedlot. Disease spread was simulated under different time dependent animal tracing scenarios. In the specific scenario modeled (incursion of FMD within a large feedlot, detection within 14 days and 90% effective tracing), simulation suggested that control costs of the outbreak significantly increase if tracing does not occur until day 10 as compared to the baseline of tracing on day 2. In addition, control costs are significantly increased if effectiveness were to drop to 30% as compared to the baseline of 90%. Results suggest potential benefits from rapid effective tracing in terms of reducing government control costs; however, a variety of other scenarios need to be explored before determining in which situations rapid effective trace-back capability is beneficial. (C) 2012 Elsevier B.V. All rights reserved. C1 [Hagerman, Amy D.] USDA, Econ Res Serv, Washington, DC USA. [Ward, Michael P.] Univ Sydney, Fac Vet Sci, Camden, NSW, Australia. [Anderson, David P.; Looney, J. Chris; McCarl, Bruce A.] Texas A&M Univ, Dept Agr Econ, College Stn, TX 77843 USA. RP Ward, MP (reprint author), Univ Sydney, Fac Vet Sci, Camden, NSW, Australia. EM michael.ward@sydney.edu.au RI Ward, Michael/C-5758-2009 FU USDA National Needs Graduate Fellowship [2007-38420-17752] FX This research was partially funded by USDA National Needs Graduate Fellowship Competitive Grant no. 2007-38420-17752. NR 15 TC 2 Z9 2 U1 0 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 J9 PREV VET MED JI Prev. Vet. Med. PD JUL 1 PY 2013 VL 110 IS 3-4 BP 323 EP 328 DI 10.1016/j.prevetmed.2012.12.011 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 164RL UT WOS:000320427100005 PM 23317567 ER PT J AU Beam, A Garber, L Sakugawa, J Kopral, C AF Beam, A. Garber, L. Sakugawa, J. Kopral, C. TI Salmonella awareness and related management practices in US urban backyard chicken flocks SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Poultry; Chickens; Urban; Biosecurity; National Animal Health Monitoring System; Survey; Salmonella; Zoonotic; Miami; Los Angeles; Denver ID AVIAN INFLUENZA; POULTRY FLOCKS; UNITED-STATES; OUTBREAKS AB Raising chickens in urban settings is a growing phenomenon in the United States. The United States Department of Agriculture's (USDA) National Animal Health Monitoring System (NAHMS) conducted a cross-sectional study to better understand health and management of privately owned chicken flocks, and Salmonella awareness among chicken owners, in three urban settings Denver, Colorado; Los Angeles, California; and Miami, Florida. Feed stores in each city were visited by data collectors during summer 2010, and customers who owned chickens were asked to complete a questionnaire. A convenience sample of 449 feed store customers was selected, and 382 (85.1%) customers participated in the study. For analysis, a stratified random sample was assumed, with the strata being individual feed stores. Median flock sizes were 5, 11 and 19 chickens in Denver, Los Angeles and Miami, respectively. In all three cities, over three-fourths of flocks contained table egg chicken breeds on the day the questionnaire was completed. In Denver, 20.4% of flocks had another species of bird present in addition to chickens, compared with 65.6% of flocks in Los Angeles and 53.6% of flocks in Miami. At the time of data collection in 2010, less than 50% of respondents in Miami and Los Angeles (40.0 and 30.2%, respectively) were aware of a connection between poultry contact, such as contact with chicks or ducks, and Salmonella infection in people, compared to 63.5% of respondents in Denver. Urban chicken flock owners who completed the questionnaire in English were more likely to be aware of the connection between poultry contact and Salmonella, compared with respondents who completed the questionnaire in Spanish (OR=3.5). The likelihood of Salmonella awareness was also higher for respondents who had heard of USDA's Biosecurity for Birds educational campaign and for respondents who sold or gave away eggs from their flocks (OR=2.5 and 2.8, respectively). Study findings demonstrate the importance of reaching the Spanish speaking population when creating educational outreach programs to reduce Salmonella infections in people who have live poultry contact. Published by Elsevier B.V. C1 [Beam, A.] Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Ctr Epidemiol, Ft Collins, CO 80526 USA. Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Ctr Anim Hlth, Ft Collins, CO 80526 USA. RP Beam, A (reprint author), Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Ctr Epidemiol, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM Andrea.L.Beam@aphis.usda.gov NR 17 TC 12 Z9 12 U1 0 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 J9 PREV VET MED JI Prev. Vet. Med. PD JUL 1 PY 2013 VL 110 IS 3-4 BP 481 EP 488 DI 10.1016/j.prevetmed.2012.12.004 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA 164RL UT WOS:000320427100021 PM 23290129 ER PT J AU Patyk, KA Helm, J Martin, MK Forde-Folle, KN Olea-Popelka, FJ Hokanson, JE Fingerlin, T Reeves, A AF Patyk, Kelly A. Helm, Julie Martin, Michael K. Forde-Folle, Kimberly N. Olea-Popelka, Francisco J. Hokanson, John E. Fingerlin, Tasha Reeves, Aaron TI An epidemiologic simulation model of the spread and control of highly pathogenic avian influenza (H5N1) among commercial and backyard poultry flocks in South Carolina, United States SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Avian influenza; H5N1; Animal disease model; Stochastic simulation; South Carolina ID MOUTH-DISEASE; RISK-FACTORS; CONFIDENCE-INTERVALS; H7N7 VIRUS; NETHERLANDS; OUTBREAK; STRATEGIES; FARMS; TRANSMISSION; INFECTION AB Epidemiologic simulation modeling of highly pathogenic avian influenza (HPAI) outbreaks provides a useful conceptual framework with which to estimate the consequences of HPAI outbreaks and to evaluate disease control strategies. The purposes of this study were to establish detailed and informed input parameters for an epidemiologic simulation model of the H5N1 strain of HPAI among commercial and backyard poultry in the state of South Carolina in the United States using a highly realistic representation of this poultry population; to estimate the consequences of an outbreak of HPAI in this population with a model constructed from these parameters; and to briefly evaluate the sensitivity of model outcomes to several parameters. Parameters describing disease state durations; disease transmission via direct contact, indirect contact, and local-area spread; and disease detection, surveillance, and control were established through consultation with subject matter experts, a review of the current literature, and the use of several computational tools. The stochastic model constructed from these parameters produced simulated outbreaks ranging from 2 to 111 days in duration (median 25 days), during which 1 to 514 flocks were infected (median 28 flocks). Model results were particularly sensitive to the rate of indirect contact that occurs among flocks. The baseline model established in this study can be used in the future to evaluate various control strategies, as a tool for emergency preparedness and response planning, and to assess the costs associated with disease control and the economic consequences of a disease outbreak. Published by Elsevier B.V. C1 [Patyk, Kelly A.] Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Ctr Epidemiol, Ft Collins, CO 80526 USA. [Patyk, Kelly A.] Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Ctr Anim Hlth, Ft Collins, CO 80526 USA. [Helm, Julie; Martin, Michael K.] Clemson Univ, Livestock Poultry Hlth Div, Columbia, SC 29229 USA. [Forde-Folle, Kimberly N.] Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Natl Ctr forAnimal Hlth Emergency Management, Ft Collins, CO 80526 USA. [Olea-Popelka, Francisco J.; Reeves, Aaron] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Clin Sci, Ft Collins, CO 80523 USA. [Hokanson, John E.; Fingerlin, Tasha] Colorado Sch Publ Hlth, Dept Epidemiol, Aurora, CO 80045 USA. RP Patyk, KA (reprint author), Vet Serv, Anim & Plant Hlth Inspect Serv, USDA, Ctr Epidemiol, 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM Kelly.A.Patyk@aphis.usda.gov OI Reeves, Aaron/0000-0001-6387-7481 NR 56 TC 4 Z9 4 U1 3 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD JUL 1 PY 2013 VL 110 IS 3-4 BP 510 EP 524 DI 10.1016/j.prevetmed.2013.01.003 PG 15 WC Veterinary Sciences SC Veterinary Sciences GA 164RL UT WOS:000320427100024 PM 23398856 ER PT J AU Moulton, MP Cropper, WP Avery, ML AF Moulton, Michael P. Cropper, Wendell P., Jr. Avery, Michael L. TI Is propagule size the critical factor in predicting introduction outcomes in passeriform birds? SO BIOLOGICAL INVASIONS LA English DT Article DE Introduced species; Passeriforms birds; Propagule pressure ID NEW-ZEALAND; ESTABLISHMENT SUCCESS; EXOTIC BIRDS; MORPHOLOGICAL ASSORTMENT; HISTORICAL RECORDS; HAWAIIAN AVIFAUNA; OCEANIC ISLANDS; GLOBAL PATTERNS; COMPETITION; PRESSURE AB Influential analyses of the propagule pressure hypothesis have been based on multiple bird species introduced to one region (e.g. New Zealand). These analyses implicitly assume that species-level and site-level characteristics are less important than the number of individuals released. In this study we compared records of passerine introductions with propagule size information across multiple regions (New Zealand, Australia, and North America). We excluded species introduced to just one of the three regions or with significant uncertainty in the historical record, as well as species that succeeded or failed in all regions. Because it is often impossible to attribute success to any single event or combination of events, our analysis compared randomly selected propagule sizes of unsuccessful introductions with those of successful introductions. Using Monte Carlo repeated sampling we found no statistical support for the propagule pressure hypothesis, even when using assumptions biased toward showing an effect. C1 [Moulton, Michael P.] Univ Florida, Dept Wildlife Ecol & Conservat, Gainesville, FL 32611 USA. [Cropper, Wendell P., Jr.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Avery, Michael L.] Natl Wildlife Res Ctr, USDA Wildlife Serv, Gainesville, FL 32641 USA. RP Moulton, MP (reprint author), Univ Florida, Dept Wildlife Ecol & Conservat, POB 110430, Gainesville, FL 32611 USA. EM moultonm@ufl.edu; wcropper@ufl.edu; michael.l.avery@aphis.usda.gov RI Cropper, Jr., Wendell/E-5952-2010; OI Cropper, Jr., Wendell/0000-0001-7851-7382; Moulton, Michael/0000-0003-4041-9343 NR 57 TC 7 Z9 7 U1 1 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD JUL PY 2013 VL 15 IS 7 BP 1449 EP 1458 DI 10.1007/s10530-012-0383-x PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 165GH UT WOS:000320470900005 ER PT J AU Keever, CC Nieman, C Ramsay, L Ritland, CE Bauer, LS Lyons, DB Cory, JS AF Keever, Carson C. Nieman, Christal Ramsay, Larissa Ritland, Carol E. Bauer, Leah S. Lyons, D. Barry Cory, Jenny S. TI Microsatellite population genetics of the emerald ash borer (Agrilus planipennis Fairmaire): comparisons between Asian and North American populations SO BIOLOGICAL INVASIONS LA English DT Article DE Microsatellite; Invasive species; Genetic diversity; Genetic bottleneck ID COLEOPTERA BUPRESTIDAE; NATURAL ENEMIES; BIOLOGY; DIFFERENTIATION; DIVERSITY; EVOLUTION; VARIANCE; SOFTWARE; CHINA; PHYLOGEOGRAPHY AB The emerald ash borer (EAB) (Agrilus planipennis Fairmaire) (Coleoptera; Buprestidae), is an invasive wood-boring beetle native to northeast Asia. This species was first detected in Michigan USA in 2002, and is a significant threat to native and ornamental ash tree species (Fraxinus spp.) throughout North America. We characterized seven polymorphic microsatellite markers for EAB and used these to investigate EAB population structure in the early invasive populations within North America and in comparison with Asia. We found 2-9 alleles per microsatellite locus, no evidence of linkage disequilibrium, and no association with known coding sequences, suggesting that these markers are suitable for population genetic analysis. Microsatellite population genetic structure was examined in 48 EAB populations sampled between 2003 and 2008 from five regions, three in the introduced range, Michigan (US) and Ontario and Quebec (Canada) and two Asian regions, China and South Korea, where EAB is native. We found significant genetic variation geographically but not temporally in EAB populations. Bayesian clustering analyses of individual microsatellite genotypes showed strong clustering among multiple North American populations and populations in both China and South Korea. Finally, allelic richness and expected heterozygosity were higher in the native range of EAB, but there was no difference in observed heterozygosity, suggesting a significant loss of alleles upon introduction but no significant change in the distribution of alleles within and among individuals. C1 [Keever, Carson C.; Cory, Jenny S.] Simon Fraser Univ, Dept Biol Sci, Burnaby, BC V5A 1S6, Canada. [Nieman, Christal; Ramsay, Larissa; Cory, Jenny S.] Algoma Univ, Dept Biol, Sault Ste Marie, ON P6A 2G4, Canada. [Nieman, Christal; Ritland, Carol E.] Univ British Columbia, Dept Forest Sci, Genet Data Ctr, Vancouver, BC V6T 1Z4, Canada. [Ramsay, Larissa; Lyons, D. Barry] Nat Resources Canada, Canadian Forest Serv, Sault Ste Marie, ON P6A 2E5, Canada. [Bauer, Leah S.] USDA, Forest Serv, No Res Stn, E Lansing, MI 48823 USA. RP Keever, CC (reprint author), Simon Fraser Univ, Dept Biol Sci, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada. EM carson.c.keever@gmail.com RI Cory, Jenny/M-9991-2013 OI Cory, Jenny/0000-0002-4637-6439 FU Canadian Food Inspection Agency; SFU FX Microsatellite development and analysis was funded by the Canadian Food Inspection Agency. Additional support was provided by SFU President's Research start-up grant. We gratefully acknowledge the assistance of G. C. Jones (CFS) in sampling EAB populations in Ontario and Michigan; H. P. Liu (Michigan State University), R. T. Gao and T. H. Zhao (Chinese Academy of Forestry), and D. L. Miller and T. Petrice (USDA FS NRS) for assisting with sampling, rearing, and maintaining EAB samples from China; D. Williams (USDA APHIS Otis) for providing EAB samples from S. Korea. We would also like to thank Michelle Franklin for her comments on the manuscript. NR 68 TC 5 Z9 5 U1 3 U2 70 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 J9 BIOL INVASIONS JI Biol. Invasions PD JUL PY 2013 VL 15 IS 7 BP 1537 EP 1559 DI 10.1007/s10530-012-0389-4 PG 23 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 165GH UT WOS:000320470900011 ER PT J AU Ly, D Hamblin, M Rabbi, I Melaku, G Bakare, M Gauch, HG Okechukwu, R Dixon, AGO Kulakow, P Jannink, JL AF Ly, Delphine Hamblin, Martha Rabbi, Ismail Melaku, Gedil Bakare, Moshood Gauch, Hugh G., Jr. Okechukwu, Richardson Dixon, Alfred G. O. Kulakow, Peter Jannink, Jean-Luc TI Relatedness and Genotype x Environment Interaction Affect Prediction Accuracies in Genomic Selection: A Study in Cassava SO CROP SCIENCE LA English DT Article ID REALIZED RELATIONSHIP MATRIX; MOLECULAR MARKERS; STATISTICAL-ANALYSIS; BREEDING VALUES; COMPLEX TRAITS; MAIZE; YIELD; POPULATIONS; RESISTANCE; REGRESSION AB Before implementation of genomic selection, evaluation of the potential accuracy of prediction can be obtained by cross-validation. In this procedure, a population with both phenotypes and genotypes is split into training and validation sets. The prediction model is fitted using the training set, and its accuracy is calculated on the validation set. The degree of genetic relatedness between the training and validation sets may influence the expected accuracy as may the genotype x environment (GxE) interaction in those sets. We developed a method to assess these effects and tested it in cassava (Manihot esculenta Crantz). We used historical phenotypic data available from the International Institute of Tropical Agriculture Genetic Gain trial and performed genotyping by sequencing for these clones. We tested cross-validation sampling schemes preventing the training and validation sets from sharing (i) genetically close clones or (ii) similar evaluation locations. For 19 traits, plot-basis heritabilities ranged from 0.04 to 0.66. The correlation between predicted and observed phenotypes ranged from 0.15 to 0.47. Across traits, predicting for less related clones decreased accuracy from 0 to 0.07, a small but consistent effect. For 17 traits, predicting for different locations decreased accuracy between 0.01 and 0.18. Genomic selection has potential to accelerate gains in cassava and the existing training population should give a reasonable estimate of future prediction accuracies. C1 [Ly, Delphine] Montpellier Supagro, F-34060 Montpellier, France. [Hamblin, Martha; Jannink, Jean-Luc] Cornell Univ, Dep Plant Breeding & Genet, Ithaca, NY 14853 USA. [Gauch, Hugh G., Jr.] Cornell Univ, Dep Crop & Soil Sci, Ithaca, NY 14853 USA. [Rabbi, Ismail; Melaku, Gedil; Bakare, Moshood; Okechukwu, Richardson; Kulakow, Peter] Int Inst Trop Agr, Ibadan, Nigeria. [Dixon, Alfred G. O.] Sierre Leone Agr Res Inst, Freetown, Sierra Leone. [Jannink, Jean-Luc] USDA ARS, RW Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Hamblin, M (reprint author), Cornell Univ, Dep Plant Breeding & Genet, Ithaca, NY 14853 USA. EM mth3@cornell.edu FU Bill and Melinda Gates Foundation FX DL and JLJ designed the study and interpreted the results. DL performed the statistical analyzes. AGOD, PK, IYR, RO, and MB selected and assembled the genetic gain collection and contributed to phenotypic evaluation. GM oversaw cassava tissue collection and DNA preparation. MTH was responsible for the GBS data and bioinformatic analyses. HGG performed the AMMI analysis. DL wrote the paper with assistance from MTH and JLJ. This work was supported by the project "Genomic Selection: The next frontier for rapid gains in maize and wheat improvement", through funds from The Bill and Melinda Gates Foundation. We thank Deniz Akdemir and Jeff Endelman for help with statistical analyses, Lisa Blanchard for preparation of GBS libraries, and Jeff Glaubitz, Rob Elshire, and Qi Sun for help with the GBS bioinformatics pipeline. NR 50 TC 27 Z9 27 U1 1 U2 43 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1312 EP 1325 DI 10.2135/cropsci2012.11.0653 PG 14 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300010 ER PT J AU Recker, JR Burton, JW Cardinal, A Miranda, L AF Recker, Jill R. Burton, Joseph W. Cardinal, Andrea Miranda, Lilian TI Analysis of Quantitative Traits in Two Long-Term Randomly Mated Soybean Populations: I. Genetic Variances SO CROP SCIENCE LA English DT Article ID RECURRENT SELECTION; REGISTRATION; PROGENY; CROSS; YIELD AB The genetic effects of long-term random mating and natural selection aided by genetic male sterility were evaluated in two soybean [Glycine max (L.) Merr.] populations: RSII and RSIII. Population means, variances, and heritabilities were estimated to determine the effects of 26 generations of random mating. The 10% highest yielding lines from each population were selected. Data was collected on flowering date, maturity date, plant height, lodging score, seed yield, seed weight, protein concentration (PC), and oil concentration (OC). RSII had a mean seed yield, PC, and OC of 2163 kg ha(-1), 416 g kg(-1), and 193 g kg(-1), respectively. Entry-mean heritability for seed yield, PC, and OC was estimated at 36, 68, and 57%, respectively. Theoretical response to selection was 183 kg ha(-1) and, after selection, the realized gain was 161 kg ha(-1). RSIII had a mean seed yield, PC, and OC of 2300 kg ha(-1), 416 g kg(-1), and 190 g kg(-1), respectively. The entry-mean heritability estimates for seed yield, PC, and OC were 57, 87, and 91%, respectively. Theoretical response to selection was 300 kg ha(-1) and, after selection, realized gain was an increase of 139 kg ha(-1). Despite not reaching their theoretical yield potential, the best lines from both populations compared well to check cultivars. The significant genetic variances and heritabilities observed demonstrate that the breakage of linkage blocks by long-term random mating and natural selection were effective in producing competitive lines. C1 [Recker, Jill R.; Cardinal, Andrea] N Carolina State Univ, Dep Crop Sci, Raleigh, NC 27695 USA. [Burton, Joseph W.; Miranda, Lilian] N Carolina State Univ, USDA ARS, Raleigh, NC 27607 USA. RP Miranda, L (reprint author), N Carolina State Univ, USDA ARS, Box 7631, Raleigh, NC 27607 USA. EM lilian.miranda@ars.usda.gov FU United Soybean Board FX We thank Dr. David Dickey, Statistics Department, North Carolina State University, for his help and guidance in analyzing the data for these experiments. Also we thank Mr. Earl Huie and all the technicians and employees of the Soybean and Nitrogen Fixation Research Unit for their help in collecting data and managing trials. This research was supported in part by the United Soybean Board. NR 20 TC 2 Z9 2 U1 0 U2 5 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1375 EP 1383 DI 10.2135/cropsci2012.10.0573 PG 9 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300016 ER PT J AU Chen, CY Nuti, RC Rowland, DL Faircloth, WH Lamb, MC Harvey, E AF Chen, Charles Y. Nuti, Russell C. Rowland, Diane L. Faircloth, Wilson H. Lamb, Marshall C. Harvey, Ernest TI Heritability and Genetic Relationships for Drought-Related Traits in Peanut SO CROP SCIENCE LA English DT Article ID CARBON-ISOTOPE DISCRIMINATION; WATER-USE EFFICIENCY; GROUNDNUT ARACHIS-HYPOGAEA; LEAF-AREA; RUNNER PEANUT; GRAIN-YIELD; TRANSPIRATION EFFICIENCY; CHLOROPHYLL-METER; HARVEST INDEX; CROP YIELD AB Water-use efficiency is often genetically correlated with specific leaf area (SLA) and leaf C isotopic composition (delta C-13) in peanut (Arachis hypogaea L.). The objectives of this study were to examine the effects of environment, genotype, and genotype x environment interaction on SLA, delta C-13, harvest index (HI), and yield and to estimate heritability and the genetic relationships. Fifteen genotypes were tested at two locations with different drought intensities for 2 yr. The additive main effects and multiplicative interaction analysis based on yield data divided eight environments (locations x treatments x years) into four clusters, which reflected a gradient in drought stress, indicating yield trait is a good indication of drought stress. The genotype Exp8-12 was identified as a best drought tolerant germplasm across environments. Heritability estimates for HI (0.68 to 0.89) and delta C-13 (0.75 to 0.89) were higher than those for SLA (0.31 to 0.88) and yield (0.12 to 0.65). A positive but small genetic correlation coefficient (r(g)) (0.26) was found between HI and yield, and low to moderate r(g) (0.14 to 0.55) were observed between HI and SLA. A relative high negative genetic correlation r(g) (-0.81 to -0.57) was found between delta C-13 and yield. In theory, the high heritability and no GxE interaction for delta C-13 make it possible a surrogate that can be used to assess drought tolerance in peanut breeding. C1 [Chen, Charles Y.; Harvey, Ernest] Auburn Univ, Dep Agron & Soil Sci, Auburn, AL 36849 USA. [Nuti, Russell C.] Dow AgroSci LLC, Shellman, GA 39886 USA. [Rowland, Diane L.] Univ Florida, Dep Agron, Gainesville, FL 32611 USA. [Faircloth, Wilson H.] Syngenta, Leesburg, GA 31763 USA. [Lamb, Marshall C.] USDA ARS, Natl Peanut Res Lab, Dawson, GA 39842 USA. RP Chen, CY (reprint author), Auburn Univ, Dep Agron & Soil Sci, Auburn, AL 36849 USA. EM cyc0002@auburn.edu NR 50 TC 3 Z9 3 U1 1 U2 24 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1392 EP 1402 DI 10.2135/cropsci2012.07.0426 PG 11 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300018 ER PT J AU Casler, MD AF Casler, Michael D. TI Partial Decomposition of the Genetic Correlation between Forage Yield and Fiber Using Semihybrids SO CROP SCIENCE LA English DT Article ID DRY-MATTER DIGESTIBILITY; NEUTRAL DETERGENT FIBER; CELL-WALL CONSTITUENTS; SMOOTH BROMEGRASS; DIVERGENT SELECTION; REED CANARYGRASS; AGRICULTURAL FITNESS; RECURRENT SELECTION; NUTRITIONAL-VALUE; ANIMAL NUTRITION AB Voluntary intake potential of a forage crop is generally considered to be the most important feed characteristic regulating animal performance. Efforts to develop forage crops with increased intake potential have been associated with reduced plant fitness, prompting a need to modify breeding objectives and methods. The objective of this study was to determine if semihyrids could be used to restore all or a portion of the forage yield lost to drift during selection for low neutral detergent fiber (NDF) in smooth bromegrass (Bromus inermis Leyss.). A total of 48 semihybrids were created by pair crossing within four base (original) populations, four high-NDF populations, and four low-NDF populations. Genetic variation declined progressively with increased NDF concentration. Midparent heterosis was positive and significant for four of six semihybrids at the low-NDF level. Two of these semihybrids also had positive and significant heterosis for NDF, reflecting the typical positive genetic correlation between yield and NDF. However, two of these semihybrids had no heterotic effect for NDF, resulting in partial decay of the genetic correlation due to a combination of linkage and drift acting on alleles that independently control these two traits. Because about one-third of this genetic correlation is malleable by manipulation of linkage and drift, there is potential to produce low-NDF smooth bromegrass populations without sacrificing forage yield potential. C1 USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Casler, MD (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM michael.casler@ars.usda.gov NR 34 TC 1 Z9 1 U1 1 U2 7 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1403 EP 1411 DI 10.2135/cropsci2013.01.0056 PG 9 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300019 ER PT J AU Khoury, CK Greene, S Wiersema, J Maxted, N Jarvis, A Struik, PC AF Khoury, Colin K. Greene, Stephanie Wiersema, John Maxted, Nigel Jarvis, Andy Struik, Paul C. TI An Inventory of Crop Wild Relatives of the United States SO CROP SCIENCE LA English DT Article ID SUNFLOWER GERMPLASM LINES; IN-SITU CONSERVATION; INTERSPECIFIC HYBRIDIZATION; TRITICUM-TAUSCHII; GENETIC-RESOURCES; HORDEUM-BULBOSUM; CLIMATE-CHANGE; INTROGRESSION LINES; WHEAT GERMPLASM; IMPROVE YIELD AB The use of crop wild relatives (CWRs) in breeding is likely to continue to intensify as utilization techniques improve and crop adaptation to climate change becomes more pressing. Significant gaps remain in the conservation of these genetic resources. As a first step toward a national strategy for the conservation of CWRs, we present an inventory of taxa occurring in the United States, with suggested prioritization of species based on potential value in crop improvement. We listed 4600 taxa from 985 genera and 194 plant families, including CWRs of potential value via breeding as well as wild species of direct use for food, forage, medicine, herb, ornamental, and/or environmental restoration purposes. United States CWRs are related to a broad range of important food, forage and feed, medicinal, ornamental, and industrial crops. Some potentially valuable species are threatened in the wild, including relatives of sunflower (Helianthus annuus L.), walnut (Juglans regia L.), pepo squash (Cucurbita pepo L.), wild rice (Zizania L.), raspberry (Rubus idaeus L.), and plum (Prunus salicina Lindl.), and few accessions of such taxa are currently conserved ex situ. We prioritize 821 taxa from 69 genera primarily related to major food crops, particularly the approximately 285 native taxa from 30 genera that are most closely related to such crops. Both the urgent collection for ex situ conservation and the management of such taxa in protected areas are warranted, necessitating partnerships between concerned organizations, aligned with regional and global initiatives to conserve and provide access to CWR diversity. C1 [Khoury, Colin K.; Jarvis, Andy] Int Ctr Trop Agr CIAT, Cali, Colombia. [Khoury, Colin K.; Struik, Paul C.] Wageningen Univ, Ctr Crop Syst Anal, NL-6708 PB Wageningen, Netherlands. [Greene, Stephanie] ARS, USDA, Prosser, WA 99350 USA. [Wiersema, John] ARS, USDA, Natl Germplasm Res Lab, Beltsville, MD 20705 USA. [Maxted, Nigel] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England. [Jarvis, Andy] CGIAR Res Program Climate Change Agr & Food Secur, Cali, Colombia. RP Khoury, CK (reprint author), Int Ctr Trop Agr CIAT, Km 17,Apartado Aereo 6713, Cali, Colombia. EM c.khoury@cgiar.org OI Wiersema, John/0000-0001-9383-2807; Khoury, Colin K./0000-0001-7893-5744 FU Global Crop Diversity Trust, Rome, Italy FX Research by the corresponding author was funded by the Global Crop Diversity Trust, Rome, Italy. We thank the NPGS curators, members of the NPGS Crop Germplasm Committees, and ARS crop experts for their inputs on the Inventory and Gary Kinard, Edward Garvey, Karen Williams, and Larry Stritch for helpful comments on the manuscript. NR 136 TC 17 Z9 17 U1 3 U2 41 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1496 EP 1508 DI 10.2135/cropsci2012.10.0585 PG 13 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300030 ER PT J AU Liu, ZL Bowden, RL Bai, GH AF Liu, Zhengli Bowden, Robert L. Bai, Guihua TI Molecular Markers for Leaf Rust Resistance Gene Lr42 in Wheat SO CROP SCIENCE LA English DT Article ID F-SP TRITICI; HEXAPLOID WHEAT; GERMPLASM; TAUSCHII; SYSTEM; MAP AB Wheat leaf rust, caused by Puccinia triticina Eriks., is an important wheat (Triticum aestivum L.) foliar disease worldwide. Growing cultivars incorporating genetic resistance is one of the most effective approaches for disease control. Leaf rust resistance gene Lr42 was identified from a wheat relative, Aegilops tauschii Coss. (goatgrass), and has been transferred into hard winter wheat. A previous study identified two markers closely linked to the gene on the short arm of chromosome 1D (1DS) using a near-isogenic population, but flanking markers for Lr42 were not found. In this study, a new mapping population was developed from a cross between 'KS93U50' (a Lr42 carrier) and a susceptible parent, 'Morocco'. An F-2 population was analyzed with all simple sequence repeat (SSR) markers available from chromosome 1D and F-2 plants and F-3 families were evaluated for seedling resistance to isolate PNMRJ, a rust isolate avirulent to Lr42. The F-2 and F-3 rust data showed that Lr42 was recessive. Seven markers formed the linkage group on chromosome 1DS. The Lr42 region was flanked by two SSR markers, Xwmc432 and Xgdm33, at 18 cM apart. The results confirmed that Lr42 is positioned on the distal end of chromosome 1DS. The flanking markers for Lr42 should be useful for map-based cloning and marker-assisted pyramiding of Lr42 with other leaf rust resistance genes. C1 [Liu, Zhengli] Hebei Acad Agr & Forestry Sci, Inst Millet Crops, Shijiazhuang 050031, Hebei, Peoples R China. [Liu, Zhengli; Bai, Guihua] Kansas State Univ, Dep Agron, Manhattan, KS 66506 USA. [Bowden, Robert L.; Bai, Guihua] ARS, USDA, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Bai, GH (reprint author), Kansas State Univ, Dep Agron, Manhattan, KS 66506 USA. EM guihua.bai@ars.usda.gov FU National Research Initiative from the USDA National Institute of Food and Agriculture [2011-68002-30029]; USDA-ARS [5430-21000-006-00D] FX This project was partially supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and USDA-ARS project 5430-21000-006-00D. The authors would like to thank Dr. Paul St. Amand, USDA Central Small Grain Genotyping Center, and Dr. Chengsong Zhu, Department of Agronomy, Kansas State University, Manhattan, KS, for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. This is contribution No. 12-414-J from the Kansas Agricultural Experiment Station, Manhattan, KS. NR 29 TC 1 Z9 1 U1 0 U2 20 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1566 EP 1570 DI 10.2135/cropsci2012.09.0532 PG 5 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300037 ER PT J AU White, JW Conley, MM AF White, Jeffrey W. Conley, Matthew M. TI A Flexible, Low-Cost Cart for Proximal Sensing SO CROP SCIENCE LA English DT Article ID PHENOMICS; WHEAT AB Increasing interest in deploying multiple types of sensing instruments for agricultural plot-level observations has created a need for simple, high-clearance vehicles that can be easily maneuvered through crops while minimizing damage due to wheel traffic. We describe a simple cart built from a 2-m-wide by 1.2-m-long steel frame that was welded onto two bicycle frames at a height providing 1 m of vertical clearance. Instruments such as radiometers and infrared thermometers are attached to the frame via arms that are secured with U-bolts. A large, horizontal surface allows mounting data loggers, batteries, or computers. The cart is easily maneuvered by one person on level ground or by two persons on terrain with furrows, berms, or other obstacles. Design sketches and lists of materials are provided in an electronic supplement. The basic design is readily modifiable for different interrow spacings and sensor positions. C1 [White, Jeffrey W.; Conley, Matthew M.] ARS, USDA, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP White, JW (reprint author), ARS, USDA, US Arid Land Agr Res Ctr, 21881 North Cardon Ln, Maricopa, AZ 85138 USA. EM Jeffrey.white@ars.usda.gov NR 10 TC 15 Z9 15 U1 1 U2 22 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1646 EP 1649 DI 10.2135/cropsci2013.01.0054 PG 4 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300046 ER PT J AU Pathak, BP Jain, M Tillman, BL Grusak, MA Gallo, M AF Pathak, Bhuvan P. Jain, Mukesh Tillman, Barry L. Grusak, Michael A. Gallo, Maria TI Effect of Gypsum Application on Mineral Composition in Peanut Pod Walls and Seeds SO CROP SCIENCE LA English DT Article ID ARACHIS-HYPOGAEA L.; REPRODUCTIVE GROWTH; DROUGHT STRESS; PLANT-GROWTH; CALCIUM; CULTIVARS; GROUNDNUT; REGISTRATION; IRRIGATION; RESPONSES AB Alleviation of soil-Ca deficiency through gypsum amendment increases the yield potential and ensures high seed quality in peanut (Arachis hypogaea L.). The effects of gypsum treatment, plant life cycle, and the fruit development stages on the accrual of several essential minerals (Ca, S, Mg, P, K, Cu, Ni, Mn, Zn, and Fe) and Na were studied in the pod walls and seeds of two runner cultivars, C-99R and Georgia Green. Calcium concentration was nearly twofold higher in pod walls than in seeds, irrespective of gypsum treatment and cultivar differences. Significant increases in Ca and S, a decrease in P concentration in pod walls and seeds, and a marginal decline in seed-Mg concentration were discernible following gypsum treatment. Sodium concentration decreased in the pod walls and Zn concentration increased in the seeds due to gypsum treatment. Progression in the plant life cycle stage was positively correlated with Ca accrual in both pod walls and seeds. The small-seeded cultivar Georgia Green showed tissue-specific increases in the acquisition of Ca, Mg, and Zn in pod walls and seeds, and a decrease in P concentration in the seeds compared with the large-seeded cv. C-99R. Compared with the respective seed maturation stages, the pod walls had lower S, Mg, P, K, and Zn, and higher Ca, Cu, Ni, Mn, Fe, and Na concentrations. While Ca, S, Mg, P, K, Zn, Fe, and Na concentrations declined, Mn concentration increased in both pod walls and seeds during fruit development. C1 [Pathak, Bhuvan P.; Jain, Mukesh; Gallo, Maria] Univ Florida, Agron Dep, Gainesville, FL 32611 USA. [Tillman, Barry L.] Univ Florida, Agron Dep, North Florida Res & Educ Ctr, Marianna, FL 32446 USA. [Grusak, Michael A.] Baylor Coll Med, Dep Pediat, USDA, ARS,Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Gallo, Maria] Univ Florida, Plant Mol & Cellular Biol Program, Genet Inst, Gainesville, FL 32610 USA. RP Gallo, M (reprint author), Univ Hawaii Manoa, Mol Biosci & Bioengn Dep, Honolulu, HI 96822 USA. EM gallom@ctahr.hawaii.edu FU Florida Peanut Producers Association; USDA-ARS [58-6250-0-008] FX Authors wish to thank J. McKinney and C. J. Boggs at the Plant Science Research and Education Unit, Citra, FL for their help with the maintenance of field plots. This research was partially supported by the Florida Peanut Producers Association, and in part by the USDA-ARS under Agreement number 58-6250-0-008 to MAG. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. NR 34 TC 1 Z9 2 U1 1 U2 15 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1658 EP 1667 DI 10.2135/cropsci2012.12.0698 PG 10 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300048 ER PT J AU Schmidt, JJ Pedersen, JF Bernards, ML Lindquist, JL AF Schmidt, Jared J. Pedersen, Jeffrey F. Bernards, Mark L. Lindquist, John L. TI Rate of Shattercane x Sorghum Hybridization In Situ SO CROP SCIENCE LA English DT Article ID MEDIATED GENE FLOW; BICOLOR L. MOENCH; CROSS-POLLINATION; POLLEN; MAIZE; FRAMEWORK; DISTANCE; HYBRIDS AB Cultivated sorghum [Sorghum bicolor (L.) Moench subsp. bicolor] can interbreed with a feral weedy relative shattercane [S. bicolor nothosubsp. drummondii (Steud.) de Wet ex Davidse]. Traits introduced from cultivated sorghum could contribute to the invasiveness of a shattercane population. An experiment was conducted to determine the potential for pollen-mediated gene flow from grain sorghum to shattercane. Shattercane with juicy midrib (dd) was planted in soybean [Glycine max (L.) Merr.] fields during 2 yr in concentric arcs at varying distances from a 0.39 ha sorghum pollen source with dry midrib (DD). The arcs were placed so that prevailing winds would carry sorghum pollen to the shattercane. Seven hundred twenty seeds from each of over 300 shattercane panicles in anthesis during sorghum pollen shed each year were collected. Progeny were evaluated by phenotype to determine rate of hybridization. Hybridization averaged 3.6% within the source in 2008 and 16.0% in 2009 and declined as distance increased. Hybridization as high as 2.6% for an individual panicle was measured at the farthest distance evaluated (200 m). Wind direction and speed were also measured and their product affected hybridization rate for all pollination periods. Results indicate that genes from cultivated sorghum will likely be introduced into shattercane populations at distances of at least 200 m and that rate of hybridization is dependent on weather factors such as wind. Source size is also important in determining hybridization rate but was not studied here. C1 [Schmidt, Jared J.; Lindquist, John L.] Univ Nebraska, Dep Agron & Hort, Lincoln, NE 68583 USA. [Bernards, Mark L.] Western Illinois Univ, Sch Agr, Macomb, IL 61455 USA. [Pedersen, Jeffrey F.] Univ Nebraska, USDA, ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Lindquist, JL (reprint author), Univ Nebraska, Dep Agron & Hort, 279 Plant Sci Hall, Lincoln, NE 68583 USA. EM jlindquist1@unl.edu NR 30 TC 3 Z9 3 U1 1 U2 8 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1677 EP 1685 DI 10.2135/cropsci2012.09.0536 PG 9 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300050 ER PT J AU Skinner, RH AF Skinner, R. Howard TI Partitioning Soil Respiration during Pasture Regrowth SO CROP SCIENCE LA English DT Article ID RHIZOSPHERE RESPIRATION; LOLIUM-PERENNE; ORGANIC-MATTER; GRASSLAND SOIL; CARBON BALANCE; ELEVATED CO2; DEFOLIATION; C-13; COMPONENTS; NITROGEN AB Understanding the regulation of net ecosystem exchange and soil C sequestration within pasture systems requires knowledge of its component fluxes, including photosynthetic uptake and respiratory loss. However, little information is available on the partitioning of soil respiration (R-S) between its heterotrophic respiration (R-H) and rhizosphere respiration (R-R) components for perennial cool-season pasture species. The purpose of this study was to use stable C isotopes to examine the effects of regrowth after defoliation on the components of soil respiration for grassland plots planted as an orchardgrass (Dactylis glomerata L.)-white clover (Trifolium repens L.) mixture but dominated by orchardgrass (90 to 100% of harvested biomass). Respiration components were quantified during the first 3 wk following defoliation at the beginning of July in 2010 and 2011. In 2010, R-S, R-H, and R-R were all lowest 1 d after cutting (DAC). Both R-S and R-H significantly increased by 8 DAC but no further increase occurred over the next 2 wk. In contrast, R-R continued to increase throughout the regrowth period. In 2011, R-S did not differ among harvests and averaged across dates was less than in 2010. During the 2011 regrowth period R-H increased slightly whereas R-R did not change significantly. The rhizosphere contribution (R-C) to R-S averaged 0.49, increasing from 0.35 to 0.56 during regrowth in 2010 but decreasing from 0.62 to 0.43 in 2011. Differences among years in respiration responses during regrowth were possibly due to a combination of factors including resource limitations, soil moisture stress, and differences in soil temperature. C1 USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Skinner, RH (reprint author), USDA ARS, Pasture Syst & Watershed Management Res Unit, Bldg 3702,Curtin Rd, University Pk, PA 16802 USA. EM howard.skinner@ars.usda.gov NR 35 TC 0 Z9 0 U1 1 U2 33 PU CROP SCIENCE SOC AMER PI MADISON PA 677 S SEGOE ROAD, MADISON, WI 53711 USA SN 0011-183X J9 CROP SCI JI Crop Sci. PD JUL-AUG PY 2013 VL 53 IS 4 BP 1791 EP 1798 DI 10.2135/cropsci2012.10.0572 PG 8 WC Agronomy SC Agriculture GA 157SB UT WOS:000319918300062 ER PT J AU Carabali-Banguero, DJ Wyckhuys, KAG Montoya-Lerma, J Kondo, T Lundgren, JG AF Carabali-Banguero, Diana J. Wyckhuys, Kris A. G. Montoya-Lerma, James Kondo, Takumasa Lundgren, Jonathan G. TI Do additional sugar sources affect the degree of attendance of Dysmicoccus brevipes by the fire ant Solenopsis geminata? SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE trophobiosis; mutualism; carbohydrates; honeydew; Hymenoptera; Formicidae; Hemiptera; Pseudococcidae ID BIOLOGICAL-CONTROL; HONEYDEW SUGARS; HOST-PLANT; HYMENOPTERA; FORMICIDAE; PSEUDOCOCCIDAE; HEMIPTERA; MUTUALISMS; APHIDS; IMPACT AB Mutualistic interactions between ants and Hemiptera are mediated to a large extent by the amount and quality of sugar-rich honeydew produced. Throughout the neotropics, the predaceous fire ant Solenopsis geminata (Fabricius) (Hymenoptera: Formicidae) is found in association with colonies of the pineapple mealybug, Dysmicoccus brevipes (Cockerell) (Hemiptera: Pseudococcidae), which they actively tend and protect from attack by natural enemies. In this study, we evaluate the effects of access to a sucrose solution on the mutualistic association between S.geminata and D.brevipes. Ten colonies of either species were established, with D.brevipes maintained on pumpkin, Cucurbita maxima Duchesne (Cucurbitaceae), in screen cages. Five of the S.geminata colonies were permitted access to vials with 20% sucrose solution and a pumpkin with 20 adult mealybugs. The remaining ant colonies were allowed access to mealybug-infested pumpkins. Ant colonies with access to the sucrose solution attended mealybugs significantly less than those without additional sugar sources. Mealybug survival rates were similar under both treatments. Total body sugars and fructose were nearly twice as high in ants with access to honeydew and sucrose vs. those with access to honeydew and water. Fructose accumulated on the pumpkins over time in both treatments, suggesting that honeydew was not fully exploited by the ants. In conclusion, D.brevipes enjoy lower degrees of ant attendance when S.geminata have alternative sources of carbohydrates. We further discuss the significance of these findings for the conservation of predaceous ants and mealybug biological control. C1 [Carabali-Banguero, Diana J.; Montoya-Lerma, James] Univ Valle, Dept Biol, Cali 25360, Colombia. [Wyckhuys, Kris A. G.] CIAT, Cali 6713, Colombia. [Kondo, Takumasa] Corp Colombiana Invest Agr CORPOICA, Palmira, Colombia. [Lundgren, Jonathan G.] ARS, USDA, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Wyckhuys, KAG (reprint author), CIAT, Apartado Aereo, Cali 6713, Colombia. EM k.wyckhuys@cgiar.org OI CARABALI BANGUERO, DIANA JOHANA/0000-0001-5383-4357; Montoya-Lerma, James/0000-0003-2122-1323 NR 53 TC 1 Z9 2 U1 2 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JUL PY 2013 VL 148 IS 1 BP 65 EP 73 DI 10.1111/eea.12076 PG 9 WC Entomology SC Entomology GA 168RU UT WOS:000320724200007 ER PT J AU Fang, XF Thornton, C Scheffler, BE Willett, KL AF Fang, Xiefan Thornton, Cammi Scheffler, Brian E. Willett, Kristine L. TI Benzo[a]pyrene decreases global and gene specific DNA methylation during zebrafish development SO ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY LA English DT Article DE Benzo[a]pyrene; DNA methylation; Zebrafish; Vasa ID GLYCINE N-METHYLTRANSFERASE; EPIGENETIC TRANSGENERATIONAL INHERITANCE; POLYCYCLIC AROMATIC-HYDROCARBONS; TELOMERASE REVERSE-TRANSCRIPTASE; BRONCHIAL EPITHELIAL-CELLS; C-MYC GENE; DIOL EPOXIDE; ENVIRONMENTAL CHEMICALS; HEPATOCELLULAR-CARCINOMA; FUNDULUS-HETEROCLITUS AB DNA methylation is important for gene regulation and is vulnerable to early-life exposure to environmental contaminants. We found that direct waterborne benzo[a]pyrene (BaP) exposure at 24 mu g/L from 2.5 to 96 hpf to zebrafish embryos significantly decreased global cytosine methylation by 44.8% and promoter methylation in uasa by 17%. Consequently, vasa expression was significantly increased by 33%. In contrast, BaP exposure at environmentally relevant concentrations did not change CpG island methylation or gene expression in cancer genes such as ras-association domain family member 1 (rassf1), telomerase reverse transcriptase (tert), c-jun, and c-myca. Similarly, BaP did not change gene expression of DNA methyltransferase 1 (dnmt1) and glycine N-methyltransferase (gnmt). While total DNMT activity was not affected, GNMT enzyme activity was moderately increased. In summary, BaP is an epigenetic modifier for global and gene specific DNA methylation status in zebrafish larvae. (C) 2013 Elsevier B.V. All rights reserved. C1 [Fang, Xiefan; Thornton, Cammi; Willett, Kristine L.] Univ Mississippi, Dept Pharmacol, Sch Pharm, University, MS 38677 USA. [Fang, Xiefan; Thornton, Cammi; Willett, Kristine L.] Univ Mississippi, Environm Toxicol Res Program, Sch Pharm, University, MS 38677 USA. [Scheffler, Brian E.] USDA ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. RP Willett, KL (reprint author), Univ Mississippi, Dept Pharmacol, Box 1848,303 Faser Hall, University, MS 38677 USA. EM kwillett@olemiss.edu RI Fang, Xiefan/G-1186-2015; OI Fang, Xiefan/0000-0002-7804-7832; Scheffler, Brian/0000-0003-1968-8952 FU National Institute of Environmental Sciences [R01ES012710, R03ES018962, R21ES019940]; South Central Technology Transfer Award from Society of Toxicology; Graduate Student Council Research Grant from the University of Mississippi [6402-21310-003-00]; Agricultural Research Service FX This project was supported in parts by Grant numbers R01ES012710, R03ES018962 and R21ES019940 from the National Institute of Environmental Sciences. It was also partly supported by South Central Technology Transfer Award from Society of Toxicology, and Graduate Student Council Research Grant from the University of Mississippi, 6402-21310-003-00 and project of the Agricultural Research Service. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Environmental Health Science, the National Institutes of Health, or the Agricultural Research Service. NR 82 TC 35 Z9 37 U1 2 U2 54 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1382-6689 J9 ENVIRON TOXICOL PHAR JI Environ. Toxicol. Pharmacol. PD JUL PY 2013 VL 36 IS 1 BP 40 EP 50 DI 10.1016/j.etap.2013.02.014 PG 11 WC Environmental Sciences; Pharmacology & Pharmacy; Toxicology SC Environmental Sciences & Ecology; Pharmacology & Pharmacy; Toxicology GA 159UV UT WOS:000320073700006 PM 23542452 ER PT J AU Kim, SI Tai, TH AF Kim, Sang-Ic Tai, Thomas H. TI High resolution genotyping by restriction enzyme-phased sequencing of advanced backcross lines of rice exhibiting differential cold stress recovery SO EUPHYTICA LA English DT Article DE Rice; Oryza sativa; Reduced representation sequencing; Seedling; Cold tolerance; Recovery ID TOLERANCE; POPULATION; LOCUS; MAP AB Advanced backcross rice lines MIb 4853-9 and 6885-2 harbor major seedling cold tolerance QTL qCTS4 and qCTS12 from the temperate japonica M202 in the genetic background of the indica IR50. Previous studies have shown that these lines exhibit the same tolerance, based on visual ratings, under constant and intermittent exposure to cold stress. However, some differences between the MIb lines with regard to physiological stress indicators are observed under constant exposure and a clear difference is detected using a cold stress recovery assay. Genotyping with over 120 SSR markers did not detect any polymorphisms. Here we report using a reduced representation sequencing method called Restriction Enzyme Site Comparative Analysis to identify M202 introgressions unique to each MIb line as candidate loci for the cold stress recovery trait. C1 [Kim, Sang-Ic; Tai, Thomas H.] Univ Calif Davis, Dept Plant Sci, USDA ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. RP Tai, TH (reprint author), Univ Calif Davis, Dept Plant Sci, USDA ARS, Crops Pathol & Genet Res Unit, Mail Stop 1, Davis, CA 95616 USA. EM Thomas.Tai@ars.usda.gov FU USDA-ARS CRIS Project [5306-21000-017-00D]; California Rice Research Board [RB-3] FX This work was supported by USDA-ARS CRIS Project 5306-21000-017-00D (T. H. T.) and California Rice Research Board Project RB-3 (T. H. T.). The authors gratefully acknowledge the technical support of P. Colowit. Thanks to Dr. V. C. Andaya and Dr. S. N. Ahn for comments on improving this manuscript. NR 15 TC 0 Z9 0 U1 1 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 J9 EUPHYTICA JI Euphytica PD JUL PY 2013 VL 192 IS 1 BP 107 EP 115 DI 10.1007/s10681-013-0894-2 PG 9 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 162QY UT WOS:000320281500011 ER PT J AU Worrall, JJ Rehfeldt, GE Hamann, A Hogg, EH Marchetti, SB Michaelian, M Gray, LK AF Worrall, James J. Rehfeldt, Gerald E. Hamann, Andreas Hogg, Edward H. Marchetti, Suzanne B. Michaelian, Michael Gray, Laura K. TI Recent declines of Populus tremuloides in North America linked to climate SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Decline; Dieback; Die-off; Drought; Climate envelope; Climatic niche ID FOREST TENT CATERPILLAR; CANADIAN ASPEN FORESTS; MOUNTAIN NATIONAL-PARK; UPPER MONTANE FORESTS; STAND-REPLACING FIRE; WESTERN CANADA; BOREAL FOREST; SOUTHWESTERN COLORADO; TREMBLING ASPEN; DROUGHT AB Populus tremuloides (trembling aspen) recently experienced extensive crown thinning, branch dieback, and mortality across North America. To investigate the role of climate, we developed a range-wide bioclimate model that characterizes climatic factors controlling distribution of aspen. We also examined indices of moisture stress, insect defoliation and other factors as potential causes of the decline. Historic climate records show that most decline regions experienced exceptionally severe drought preceding the recent episodes. The bioclimate model, driven primarily by maximum summer temperatures and April-September precipitation, shows that decline tended to occur in marginally suitable habitat, and that climatic suitability decreased markedly in the period leading up to decline in almost all decline regions. Other factors, notably multi-year defoliation by tent caterpillars (Malacosoma spp.) and stem damage by fungi and insects, also play a substantial role in decline episodes, and may amplify or prolong the impacts of moisture stress on aspen over large areas. Many severely affected stands have poor regeneration potential, raising concerns that increasing aridity could ultimately lead to widespread loss of aspen forest cover. The analysis indicates that exceptional droughts were a major cause of the decline episodes, especially in the drier regions, and that aspen is sensitive to drought in much of its range. Coupling the bioclimate model with climate projections suggests that we should expect substantial loss of suitable habitat within the current distribution, especially in the USA and Mexico. Published by Elsevier B.V. C1 [Worrall, James J.; Marchetti, Suzanne B.] US Forest Serv, Gunnison, CO 81230 USA. [Rehfeldt, Gerald E.] US Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Hamann, Andreas; Gray, Laura K.] Univ Alberta, Dept Renewable Resources, Edmonton, AB T6G 2H1, Canada. [Hogg, Edward H.; Michaelian, Michael] No Forestry Ctr, Canadian Forest Serv, Edmonton, AB T6H 3S5, Canada. RP Worrall, JJ (reprint author), US Forest Serv, 216 N Colorado St, Gunnison, CO 81230 USA. EM jworrall@fs.fed.us OI Gray, Laura/0000-0003-0477-2705; Hamann, Andreas/0000-0003-2046-4550 NR 104 TC 69 Z9 69 U1 5 U2 95 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD JUL 1 PY 2013 VL 299 SI SI BP 35 EP 51 DI 10.1016/j.foreco.2012.12.033 PG 17 WC Forestry SC Forestry GA 167LT UT WOS:000320634200005 ER PT J AU Mello, CR Viola, MR Beskow, S Norton, LD AF Mello, C. R. Viola, M. R. Beskow, S. Norton, L. D. TI Multivariate models for annual rainfall erosivity in Brazil SO GEODERMA LA English DT Article DE Soil erosion; Rainfall erosivity; GIS; Regression models; Regression-kriging; Brazilian regions ID RETURN PERIOD; MONTHLY PRECIPITATION; SOIL-EROSION; INTERPOLATION METHODS; SPATIAL VARIABILITY; MATO-GROSSO; EI30 INDEX; REGRESSION; PATTERNS; STATE AB Water erosion has been considered as the most important worldwide environmental problem, being especially caused by intense rainfall events. The potential of rain to generate soil erosion is known as rainfall erosivity and its estimation is fundamental for the understanding of climatic vulnerability of a given region. This work aims to develop models for estimating mean annual rainfall erosivity for Brazilian regions based on multiple linear regression, using latitude, longitude and altitude as predictors for the models. Equations for rainfall erosivity estimations as function of the Modified Fournier Index (MFI) were acquired from 54 Brazilian pluviographic stations (termed as "Main Stations" in this work) to generate the database for this study. These equations were applied to estimate mean annual rainfall erosivity for 773 different rain gauges taking into account historical series with at least 15 consecutive years of daily precipitation and considering the similarity of the Precipitation Concentration Index (PCI). These rain gauges contain only pluviometric records, thus allowing the rainfall erosivity calculation in function of MFI in accordance with the order of PCI. The goodness-of-fit of each model was evaluated taking into account the adjusted coefficient of determination and the significance level of each variable. Moreover, the mean absolute error, bias of estimation, and the residual probability distribution were evaluated for other 155 rain gauges which were used exclusively for validation. All the adjusted multivariate models presented acceptable values for the statistical coefficients, being possible to estimate the mean annual rainfall erosivity for any location in Brazil using only its geographical coordinates and altitude. An annual rainfall erosivity map was created for Brazil based on the multivariate models and ordinary kriging map for residuals derived from the models (regression-kriging technique). It can be concluded that this map resulted in a spatial distribution of Elm better than the former map and, in addition, it can be considered an important update related to the rainfall erosivity study in Brazil since more representative data sets were used. (C) 2013 Elsevier B.V. All rights reserved. C1 [Mello, C. R.; Viola, M. R.] Univ Fed Lavras, Dept Engn, BR-37200000 Laval, MG, Brazil. [Beskow, S.] Univ Fed Pelotas, Ctr Technol Dev Water Resources Engn, BR-96010970 Pelotas, RS, Brazil. [Norton, L. D.] Purdue Univ, USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Mello, CR (reprint author), Univ Fed Lavras, Dept Engn, CP 3037, BR-37200000 Laval, MG, Brazil. EM crmello@deg.ufla.br RI de Mello, Carlos/G-5821-2012; Viola, Marcelo/F-7019-2012 OI de Mello, Carlos/0000-0002-6033-5342; NR 77 TC 17 Z9 20 U1 0 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD JUL PY 2013 VL 202 BP 88 EP 102 DI 10.1016/j.geoderma.2013.03.009 PG 15 WC Soil Science SC Agriculture GA 164PZ UT WOS:000320423300011 ER PT J AU Jun, TH Mian, MAR Michel, AP AF Jun, T-H Mian, M. A. Rouf Michel, A. P. TI Genetic mapping of three quantitative trait loci for soybean aphid resistance in PI 567324 SO HEREDITY LA English DT Article DE soybean; aphid resistance; quantitative trait loci; antixenosis; antibiosis ID QTLS; INHERITANCE; POPULATION; DISCOVERY; BIOTYPES; FIELD; MAP AB Host-plant resistance is an effective method for controlling soybean aphid (Aphis glycines Matsumura), the most damaging insect pest of soybean (Glycine max (L.) Merr.) in North America. Recently, resistant soybean lines have been discovered and at least four aphid resistance genes (Rag1, Rag2, Rag3 and rag4) have been mapped on different soybean chromosomes. However, the evolution of new soybean aphid biotypes capable of defeating host-plant resistance conferred by most single genes demonstrates the need for finding germplasm with multigenic resistance to the aphid. This study was conducted to map quantitative trait loci (QTL) for aphid resistance in PI 567324. We identified two major QTL (QTL_13_1 and QTL_13_2) for aphid resistance on soybean chromosome 13 using 184 recombinant inbred lines from a 'Wyandot' x PI 567324 cross. QTL_13_1 was located close to the previously reported Rag2 gene locus, and QTL_13_2 was close to the rag4 locus. A minor QTL (QTL_6_1) was also detected on chromosome 6, where no gene for soybean aphid resistance has been reported so far. These results indicate that PI 567324 possesses oligogenic resistance to the soybean aphid. The molecular markers closely linked to the QTL reported here will be useful for development of cultivars with oligogenic resistance that are expected to provide broader and more durable resistance against soybean aphids compared with cultivars with monogenic resistance. C1 [Jun, T-H; Michel, A. P.] Ohio State Univ OARDC, Dept Entomol, Wooster, OH USA. [Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, USDA ARS, Wooster, OH 44691 USA. RP Mian, MAR (reprint author), Ohio State Univ, Dept Hort & Crop Sci, USDA ARS, 1680 Madison Ave, Wooster, OH 44691 USA. EM mian.3@osu.edu FU UDSA-ARS; OSU/OARDC; North Central Soybean Research Program FX We thank Jane Todd, Keith Freewalt, Ronald Fioritto and Tim Mendiola for their technical help in this study. We also thank the employees at the MCIC for their help in SNP genotyping. We appreciate Professor Anne Dorrance for providing the SSR primers with the 'OSU' prefix. This study was supported by UDSA-ARS, The OSU/OARDC and a grant from North Central Soybean Research Program. NR 31 TC 9 Z9 12 U1 1 U2 37 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X J9 HEREDITY JI Heredity PD JUL PY 2013 VL 111 IS 1 BP 16 EP 22 DI 10.1038/hdy.2013.10 PG 7 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA 163HK UT WOS:000320326500003 PM 23486080 ER PT J AU Smith, AD Cheung, LM Beshah, E Shea-Donohue, T Urban, JF AF Smith, Allen D. Cheung, Lumei Beshah, Ethiopia Shea-Donohue, Terez Urban, Joseph F., Jr. TI Selenium Status Alters the Immune Response and Expulsion of Adult Heligmosomoides bakeri Worms in Mice SO INFECTION AND IMMUNITY LA English DT Article ID SYSTEMIC INFLAMMATORY RESPONSE; LUNG ADENOCARCINOMA CELLS; GLUTATHIONE-PEROXIDASE; NEMATODE PARASITES; VITAMIN-E; DEFICIENCY INCREASES; HELMINTH INFECTION; COXSACKIEVIRUS B3; GENE-EXPRESSION; SELENOPROTEINS AB Heligmosomoides bakeri is a nematode with parasitic development exclusively in the small intestine of infected mice that induces a potent STAT6-dependent Th2 immune response. We previously demonstrated that host protective expulsion of adult H. bakeri worms from a challenge infection was delayed in selenium (Se)-deficient mice. In order to explore mechanisms associated with the delayed expulsion, 3-week-old female BALB/c mice were placed on a torula yeast-based diet with or without 0.2 ppm Se, and after 5 weeks, they were inoculated with H. bakeri infective third-stage larvae (L3s). Two weeks after inoculation, the mice were treated with an anthelmintic and then rested, reinoculated with L3s, and evaluated at various times after reinoculation. Analysis of gene expression in parasite-induced cysts and surrounding tissue isolated from the intestine of infected mice showed that the local-tissue Th2 response was decreased in Se-deficient mice compared to that in Se-adequate mice. In addition, adult worms recovered from Se-deficient mice had higher ATP levels than worms from Se-adequate mice, indicating greater metabolic activity in the face of a suboptimal Se-dependent local immune response. Notably, the process of worm expulsion was restored within 2 to 4 days after feeding a Se-adequate diet to Se-deficient mice. Expulsion was associated with an increased local expression of Th2-associated genes in the small intestine, intestinal glutathione peroxidase activity, secreted Relm-beta protein, anti-H. bakeri IgG1 production, and reduced worm fecundity and ATP-dependent metabolic activity. C1 [Smith, Allen D.; Cheung, Lumei; Beshah, Ethiopia; Urban, Joseph F., Jr.] USDA ARS, Beltsville Agr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Shea-Donohue, Terez] Univ Maryland, Sch Med, Dept Med, Mucosal Biol Ctr, Baltimore, MD 21201 USA. RP Smith, AD (reprint author), USDA ARS, Beltsville Agr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. EM allen.smith@ars.usda.gov OI Urban, Joseph/0000-0002-1590-8869 FU United States Department of Agriculture, Agricultural Research Service [1235-51000-055] FX Funding for this research was provided by the United States Department of Agriculture, Agricultural Research Service, project number 1235-51000-055. NR 54 TC 4 Z9 5 U1 0 U2 12 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 J9 INFECT IMMUN JI Infect. Immun. PD JUL PY 2013 VL 81 IS 7 BP 2546 EP 2553 DI 10.1128/IAI.01047-12 PG 8 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA 163AI UT WOS:000320307300027 PM 23649095 ER PT J AU Leij, FJ Bradford, SA AF Leij, Feike J. Bradford, Scott A. TI Colloid transport in dual-permeability media SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Analytical solution; Colloid retention; Optimization; Solute transport ID SATURATED POROUS-MEDIA; SOLUTE TRANSPORT; FECAL BACTERIA; WATER-FLOW; SOILS; MOVEMENT; MODELS; PRINCIPLES; MACROPORES; ATTACHMENT AB It has been widely reported that colloids can travel faster and over longer distances in natural structured porous media than in uniform structureless media used in laboratory studies. The presence of preferential pathways for colloids in the subsurface environment is of concern because of the increased risks for disease caused by microorganisms and colloid-associated contaminants. This study presents a model for colloid transport in dual-permeability media that includes reversible and irreversible retention of colloids and first-order exchange between the aqueous phases of the two regions. The model may also be used to describe transport of other reactive solutes in dual-permeability media. Analytical solutions for colloid concentrations in aqueous and solid phases were obtained using Laplace transformation and matrix decomposition. The solutions proved convenient to assess the effect of model parameters on the colloid distribution. The analytical model was used to describe effluent concentrations for a bromide tracer and 3.2- or 1-mu m-colloids that were observed after transport through a composite 10-cm long porous medium made up of a cylindrical lens or core of sand and a surrounding matrix with sand of a different grain size. The tracer data were described very well and realistic estimates were obtained for the pore-water velocity in the two flow domains. An accurate description was also achieved for most colloid breakthrough curves. Dispersivity and retention parameters were typically greater for the larger 3.2-mu m-colloids while both reversible and irreversible retention rates tended to be higher for the finer sands than the coarser sand. The relatively small sample size and the complex flow pattern in the composite medium made it difficult to reach definitive conclusions regarding transport parameters for colloid transport. (C) 2013 Elsevier B.V. All rights reserved. C1 [Leij, Feike J.] Calif State Univ Long Beach, Dept Civil Engn & Construct Engn Management, Long Beach, CA 90840 USA. [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. RP Leij, FJ (reprint author), Calif State Univ Long Beach, Dept Civil Engn & Construct Engn Management, 1250 Bellflower Blvd MS5101,VEC 206, Long Beach, CA 90840 USA. EM Feike.Leij@csulb.edu; Scott.Bradford@ars.usda.gov NR 42 TC 5 Z9 5 U1 4 U2 54 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD JUL PY 2013 VL 150 BP 65 EP 76 DI 10.1016/j.jconhyd.2013.03.010 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA 160AP UT WOS:000320088700007 PM 23676298 ER PT J AU Tammen, SA Friso, S Choi, SW AF Tammen, Stephanie A. Friso, Simonetta Choi, Sang-Woon TI Epigenetics: The link between nature and nurture SO MOLECULAR ASPECTS OF MEDICINE LA English DT Review DE Epigenetics; DNA methylation; Histone modification; Chromatin remodeling; microRNA; Nutrition; Environment ID CHROMATIN REMODELING ACTIVITIES; TUMOR-SUPPRESSIVE MICRORNAS; X-CHROMOSOME INACTIVATION; DNA METHYLATION; HISTONE H3; GENE-EXPRESSION; LYSINE-4 METHYLATION; ENZYMATIC-PROPERTIES; COLORECTAL-CANCER; TEA POLYPHENOL AB While the eukaryotic genome is the same throughout all somatic cells in an organism, there are specific structures and functions that discern one type of cell from another. These differences are due to the cell's unique gene expression patterns that are determined during cellular differentiation. Interestingly, these cell-specific gene expression patterns can be affected by an organism's environment throughout its lifetime leading to phenotypical changes that have the potential of altering risk of some diseases. Both cell-specific gene expression signatures and environment mediated changes in expression patterns can be explained by a complex network of modifications to the DNA, histone proteins and degree of DNA packaging called epigenetic marks. Several areas of research have formed to study these epigenetic modifications, including DNA methylation, histone modifications, chromatin remodeling and microRNA (miRNA). The original definition of epigenetics incorporates inheritable but reversible phenomena that affect gene expression without altering base pairs. Even though not all of the above listed epigenetic traits have demonstrated heritability, they can all alter gene transcription without modification to the underlying genetic sequence. Because these epigenetic patterns can also be affected by an organism's environment, they serve as an important bridge between life experiences and phenotypes. Epigenetic patterns may change throughout one's lifespan, by an early life experience, environmental exposure or nutritional status. Epigenetic signatures influenced by the environment may determine our appearance, behavior, stress response, disease susceptibility, and even longevity. The interaction between types of epigenetic modifications in response to environmental factors and how environmental cues affect epigenetic patterns will further elucidate how gene transcription can be affectively altered. (C) 2012 Elsevier Ltd. All rights reserved. C1 [Tammen, Stephanie A.; Choi, Sang-Woon] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tammen, Stephanie A.; Choi, Sang-Woon] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Friso, Simonetta] Univ Verona, Sch Med, I-37100 Verona, Italy. RP Choi, SW (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM stephanie.tammen@tufts.edu; simonetta.friso@univr.it; sang.choi@tufts.edu FU US Department of Agriculture [51000-074-01S]; National Institute of Health [R01 AG025834] FX This material is based upon work supported by the US Department of Agriculture, under Agreement No. 51000-074-01S. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the US Dept of Agriculture. This project has been supported in part by the National Institute of Health Grants R01 AG025834 (S.W.C.). Authors do not have any conflict of interest. NR 108 TC 60 Z9 65 U1 14 U2 141 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD JUL-AUG PY 2013 VL 34 IS 4 SI SI BP 753 EP 764 DI 10.1016/j.mam.2012.07.018 PG 12 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA 165KI UT WOS:000320482400001 PM 22906839 ER PT J AU Udali, S Guarini, P Moruzzi, S Choi, SW Friso, S AF Udali, Silvia Guarini, Patrizia Moruzzi, Sara Choi, Sang-Woon Friso, Simonetta TI Cardiovascular epigenetics: From DNA methylation to microRNAs SO MOLECULAR ASPECTS OF MEDICINE LA English DT Review DE Epigenetics; DNA methylation; Histone modifications; MicroRNA; Nutritional epigenetics; Gene-nutrient interactions; Cardiovascular disease; Coronary artery disease; Stroke; Hypertension; Heart failure ID ACUTE MYOCARDIAL-INFARCTION; CORONARY-ARTERY-DISEASE; NITRIC-OXIDE SYNTHASE; TRANSIENT FOCAL ISCHEMIA; SMOOTH-MUSCLE-CELLS; HUMAN HEART-FAILURE; CIRCULATING MICRORNAS; PERIPHERAL-BLOOD; GENE PROMOTER; EMERGING ROLE AB Epigenetic phenomena are defined as heritable mechanisms that establish and maintain mitotically stable patterns of gene expression without modifying the base sequence of DNA. The major epigenetic features of mammalian cells include DNA methylation, post-translational histone modifications and RNA-based mechanisms including those controlled by small non-coding RNAs (miRNAs). The impact of epigenetic mechanisms in cardiovascular pathophysiology is now emerging as a major player in the interface between genotype to phenotype variability. This topic of research has strict implications on disease development and progression, and opens up possible novel preventive strategies in cardiovascular disease. An important aspect of epigenetic mechanisms is that they are potentially reversible and may be influenced by nutritional-environmental factors and through gene-environment interactions, all of which have an important role in complex, multifactorial diseases such as those affecting the cardiovascular system. Gene expression regulation through the interplay of DNA methylation and histone modifications is well-established, although the knowledge about the function of epigenetic signatures in cardiovascular disease is still largely unexplored. The study of epigenetic markers is, therefore, a very promising frontier of science which may aid in a deeper understanding of molecular mechanisms underlying the modulation of gene expression in the biomolecule pathways linked to cardiovascular diseases. This review focuses on up-to-date knowledge pertaining to the role of epigenetics, from DNA methylation to miRNAs, in major cardiovascular diseases such as ischemic heart disease, hypertension, heart failure and stroke. (C) 2012 Published by Elsevier Ltd. C1 [Udali, Silvia; Guarini, Patrizia; Moruzzi, Sara; Friso, Simonetta] Univ Verona, Sch Med, Dept Med, I-37134 Verona, Italy. [Choi, Sang-Woon] Tufts Univ, Vitamins & Carcinogenesis Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Friso, S (reprint author), Univ Verona, Sch Med, Dept Med, Policlin GB Rossi, Ple LA Scuro 10, I-37134 Verona, Italy. EM simonetta.friso@univr.it FU Cariverona Foundation, Verona, Italy FX This project has been partly supported by the Cariverona Foundation, Verona, Italy (to S.F.). Authors do not declare any conflict of interest related to the present work. NR 118 TC 48 Z9 51 U1 4 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD JUL-AUG PY 2013 VL 34 IS 4 SI SI BP 883 EP 901 DI 10.1016/j.mam.2012.08.001 PG 19 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA 165KI UT WOS:000320482400010 PM 22981780 ER PT J AU Young, MK McKelvey, KS Pilgrim, KL Schwartz, MK AF Young, Michael K. McKelvey, Kevin S. Pilgrim, Kristine L. Schwartz, Michael K. TI DNA barcoding at riverscape scales: assessing biodiversity among fishes of the genus Cottus (Teleostei) in northern Rocky Mountain streams SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE Cottidae; cryptic species; sculpins; species discovery ID FRESH-WATER FISHES; MITOCHONDRIAL-DNA; PHYLOGENETIC INFERENCE; MOLECULAR SYSTEMATICS; SPECIES DELIMITATION; APPALACHIAN STREAM; MOTTLED SCULPIN; TAXONOMY; CONSERVATION; PHYLOGEOGRAPHY AB There is growing interest in broad-scale biodiversity assessments that can serve as benchmarks for identifying ecological change. Genetic tools have been used for such assessments for decades, but spatial sampling considerations have largely been ignored. Here, we demonstrate how intensive sampling efforts across a large geographical scale can influence identification of taxonomic units. We used sequences of mtDNA cytochrome c oxidase subunit 1 and cytochrome b, analysed with maximum parsimony networks, maximum-likelihood trees and genetic distance thresholds, as indicators of biodiversity and species identity among the taxonomically challenging fishes of the genus Cottus in the northern Rocky Mountains, USA. Analyses of concatenated sequences from fish collected in all major watersheds of this area revealed eight groups with species-level differences that were also geographically circumscribed. Only two of these groups, however, were assigned to recognized species, and these two assignments resulted in intraspecific genetic variation (>2.0%) regarded as atypical for individual species. An incomplete inventory of individuals from throughout the geographical ranges of many species represented in public databases, as well as sample misidentification and a poorly developed taxonomy, may have hampered species assignment and discovery. We suspect that genetic assessments based on spatially robust sampling designs will reveal previously unrecognized biodiversity in many other taxa. C1 [Young, Michael K.; McKelvey, Kevin S.; Pilgrim, Kristine L.; Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Young, MK (reprint author), US Forest Serv, Rocky Mt Res Stn, 800 East Beckwith Ave, Missoula, MT 59801 USA. EM mkyoung@fs.fed.us RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 NR 90 TC 13 Z9 13 U1 1 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD JUL PY 2013 VL 13 IS 4 BP 583 EP 595 DI 10.1111/1755-0998.12091 PG 13 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA 164GE UT WOS:000320396300004 PM 23496857 ER PT J AU Miles, DM Brooks, JP McLaughlin, MR Rowe, DE AF Miles, D. M. Brooks, J. P. McLaughlin, M. R. Rowe, D. E. TI Broiler litter ammonia emissions near sidewalls, feeders, and waterers SO POULTRY SCIENCE LA English DT Article DE ammonia; broiler; litter; rice hull; waterer ID SPATIAL VARIABILITY; MOISTURE-CONTENT; MASS-BALANCE; VOLATILIZATION; CHICKENS; HOUSES; CAKE AB Ammonia (NH3) volatilized from broiler litter diminishes indoor air quality, which can potentially decrease bird productivity. Emissions of NH3 exhausted from broiler houses pose environmental concerns for ecosystem biodiversity, aquatic nutrient enrichment, and particulate formation in the atmosphere. Research was conducted sampling litter (rice hull base) in 3 tunnel-ventilated commercial broiler houses during wk 3 (mid-growout) of 6 flocks. The purpose was to assess NH3 generated near the sidewalls, waterers, and feeders. Litter samples (100 g) were placed in chambers receiving constant air flow. Boric acid (H3BO3) titration each 24 h for 4 d was used to determine NH3 volatilized from the samples. Litter located near waterers emitted the most cumulative NH3 (approximately 12.3 mg of N.kg of litter(-1).h(-1)) with less NH3 associated with feeders and sidewalls (2.9 to 7.6 mg of N.kg of litter(-1).h(-1)). Moisture content of litter samples was greatest at waterers (45%) followed by sidewalls (26%) and feeders (20%). In addition, litter pH at the sidewalls and feeders could be predicted by linear equations associated with the number of flocks on the litter. At the waterers, litter pH was differentiated based on the half of house where higher litter pH existed in the nonbrood half (8.55 vs. 8.13). The results indicate that controlling NH3 near watering lines to a level consistent with feeding lines and near the house wall could reduce NH3 generated by 38 to 77%. These findings support efforts for NH3 control at mid-growout, especially considering zone litter treatments near waterers and appropriate attention to waterer management. C1 [Miles, D. M.; Brooks, J. P.; McLaughlin, M. R.] USDA ARS, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. [Rowe, D. E.] Mississippi State Univ, Dept Expt Stat, Mississippi State, MS 39762 USA. RP Miles, DM (reprint author), USDA ARS, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. EM dana.miles@ars.usda.gov FU USDA, Agricultural Research Service FX Funding for the research was provided by the USDA, Agricultural Research Service. NR 28 TC 7 Z9 7 U1 1 U2 35 PU POULTRY SCIENCE ASSOC INC PI SAVOY PA 1111 N DUNLAP AVE, SAVOY, IL 61874-9604 USA SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD JUL PY 2013 VL 92 IS 7 BP 1693 EP 1698 DI 10.3382/ps.2012-02809 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA 164WV UT WOS:000320443400001 PM 23776254 ER PT J AU Rice, PJ Horgan, BP AF Rice, Pamela J. Horgan, Brian P. TI Evaluation of nitrogen and phosphorus transport with runoff from fairway turf managed with hollow tine core cultivation and verticutting SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Nitrogen; Phosphorus; Turf; Runoff; Water quality ID CREEPING BENTGRASS; SOIL-EROSION; THATCH ACCUMULATION; CULTURAL-PRACTICES; BERMUDAGRASS TURF; SURFACE RUNOFF; PUTTING GREEN; TURFGRASS; NUTRIENT; QUALITY AB Enrichment of surface waters with excess nutrients is associated with increased algal blooms, euthrophication and hypoxic zones, as reported in the northern Gulf of Mexico. A source of nutrients to surface waters results from fertilizer runoff. Management strategies used to maintain turf on golf courses and recreational fields often include aerification and application of fertilizer. Although research exists on benefits of core cultivation and verticutting (VC) to reduce thatch and the transport of applied chemicals with runoff, there are no studies reporting the effect of coupling these management practices with the goal of further reduction of off-site transport of fertilizer with runoff. We hypothesized that the addition of VC to hollow tine core cultivation (HTCC) would enhance infiltration of precipitation, reduce runoff and nutrient transport with runoff and therefore influence concentrations of nutrients in surface waters receiving runoff from turf managed as a golf course fairway. Greater runoff and mass of soluble phosphorus and ammonium nitrogen transported with runoff were measured from plots managed with HTCC + VC than HTCC; however, the reverse was noted for nitrate nitrogen. Only a portion of the observed trends proved to be statistically significant. Our research showed no reduction or enhancement of risk associated with surface water concentrations of phosphorus or nitrogen, resulting from runoff from creeping bentgrass turf that was managed with HTCC+VC compared to HTCC. Data obtained in this research will be useful to grounds superintendents when selecting best management practices and to scientists seeking data relating runoff to land management for watershed-scale modeling. Published by Elsevier B.V. C1 [Rice, Pamela J.] ARS, USDA, St Paul, MN 55108 USA. [Horgan, Brian P.] Univ Minnesota, Dept Hort, St Paul, MN 55108 USA. RP Rice, PJ (reprint author), ARS, USDA, 1991 Upper Borlaug Hall,Room 439, St Paul, MN 55108 USA. EM pamela.rice@ars.usda.gov; bphorgan@umn.edu FU U.S. Golf Association, Green Section Research FX The present research was funded in part by the U.S. Golf Association, Green Section Research. We thank C. Borgen, M. Dolan, S. Greseth, A. Hollman, C. Krueger, J. Lanners, M. McNearney, J. Rittenhouse, C. Rosen, A. Seeley, and Nelson Irrigation. NR 73 TC 2 Z9 2 U1 4 U2 69 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD JUL 1 PY 2013 VL 456 BP 61 EP 68 DI 10.1016/j.scitotenv.2013.02.051 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA 163NH UT WOS:000320343700008 PM 23584034 ER PT J AU Pogue, MG AF Pogue, Michael G. TI Revised status of Chloridea Duncan and (Westwood), 1841, for the Heliothis virescens species group (Lepidoptera: Noctuidae: Heliothinae) based on morphology and three genes SO SYSTEMATIC ENTOMOLOGY LA English DT Article AB The Heliothinae comprise some of the world's most injurious agricultural pests. This study reanalyses a subsample of the Heliothis group to determine the monophyly of Chloridea (Heliothis virescens and H.subflexa). Two nuclear gene regions, elongation factor-1 (EF-1; 1240bp) and dopa decarboylase (DDC; 687bp), and the barcoding region of mitochondrial cytochrome oxidase I (COI; 708bp) were used in this analysis for a total of 2635bp and a morphological dataset of 20 characters and 62 character states. Sixteen species representing five genera plus two outgroup species were used in the analysis. Analyses used were Maximum Parsimony (MP), Maximum Likelihood (ML) and Bayesian Inference (BI). The revised status for the monophyletic genus Chloridea Duncan and (Westwood) was supported by a very strong bootstrap support (BP=98-100). Larval host-plant usage is discussed within the Heliothis clade. Polyphagy is most likely the ancestral condition with a host shift to monophagy and oligophagy. Based on known larval hosts, Heliocheilus is oligophagous on Poaceae. Traits of host plant use in Helicoverpa and Chloridea where both polyphagy and oligophagy occur in closely related species are discussed. C1 [Pogue, Michael G.] ARS, USDA, Systemat Entomol Lab, PSI,US, Washington, DC 20013 USA. RP Pogue, MG (reprint author), ARS, USDA, Systemat Entomol Lab, PSI,US,Smithsonian Inst,NMNH, POB 37012,MRC 168, Washington, DC 20013 USA. EM michael.pogue@ars.usda.gov NR 0 TC 6 Z9 6 U1 2 U2 16 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6970 J9 SYST ENTOMOL JI Syst. Entomol. PD JUL PY 2013 VL 38 IS 3 BP 523 EP 542 DI 10.1111/syen.12010 PG 20 WC Evolutionary Biology; Entomology SC Evolutionary Biology; Entomology GA 166MX UT WOS:000320560100006 ER PT J AU Rightmyer, MG Griswold, T Brady, SG AF Rightmyer, Molly G. Griswold, Terry Brady, Sean G. TI Phylogeny and systematics of the bee genus Osmia (Hymenoptera: Megachilidae) with emphasis on North American Melanosmia: subgenera, synonymies and nesting biology revisited SO SYSTEMATIC ENTOMOLOGY LA English DT Article ID MULTIPLE SEQUENCE ALIGNMENT; CONVERGENT EVOLUTION; MITOCHONDRIAL; BIOGEOGRAPHY; POLLINATORS; INFERENCE; ACCURACY; APOIDEA AB The predominantly Holarctic bee genus Osmia Panzer is species-rich and behaviourally diverse. A robust phylogeny of this genus is important for understanding the evolution of the immense variety of morphological and behavioural traits exhibited by this group. We infer a phylogeny of Osmia using DNA sequence data obtained from three nuclear genes (elongation factor 1-, LW-rhodopsin and CAD) and the mitochondrial gene COI. Our taxon sampling places special attention on North American members of the subgenus Melanosmia Schmiedeknecht; we discuss the novel placement of a number of species traditionally assigned to O. (Melanosmia) and examine the relative support for alternative classifications of this species-rich subgenus. We use this new phylogeny to guide a reassessment of morphological and behavioural characters within Osmia. Our results provide support for the recognition of Osmia (Hapsidosmia), subgen.n., a monotypic subgenus containing Osmia iridis Cockerell & Titus. We synonymize Osmia (Mystacosmia) Snelling under O. (Melanosmia), syn.n. We synonymize Osmia (Acanthosmioides) Ashmead under O. (Melanosmia), syn.n., propose odontogaster species group' as a replacement for the subgeneric name Acanthosmioides, and refine the morphological characters that serve to diagnose the species group. We additionally propose nigrifrons species group' for a clade within O. (Melanosmia) containing most species formerly placed in Osmia (Centrosmia) Robertson. We demonstrate more cohesive patterns of nest substrate use in the nigrifrons and odontogaster species groups than was previously believed to occur, reconsider character polarity of aspects of the female mandible, and show that a large number of morphological characters have evolved convergently within the genus. In order to facilitate discussion of relevant taxa, we propose the following 15 new synonymies: O. bakeri Sandhouse under O. melanopleura Cockerell; O. crenulaticornis Michener under O. pinorum Cockerell; O. claremontensis Michener under O. sedula Sandhouse; O. cockerelli Sandhouse under O. dakotensis Michener; O. francisconis White under O. enixa Sandhouse; O. hurdi White under O. austromaritima Michener; O. sladeni Sandhouse under O. nifoata Cockerell; O. titusi Cockerell under O. phenax Cockerell; O. subtrevoris Cockerell, O. physariae Cockerell, and O. erecta Michener under O. giliarum Cockerell; and O. universitatis Cockerell, O. integrella Cockerell, O. amala Cockerell, and O. metitia Cockerell under O. nigrifrons Cresson, syn.n. We remove O. wyomingensis Michener from synonymy with O. nifoata Cockerell, stat.n., and O. pinorum Cockerell from synonymy with O. physariae Cockerell, stat.n. This published work has been registered in ZooBank, http://zoobank.org/urn:lsid:zoobank.org:pub:A3E7D63B-5C4C-4ACF-BF33-48E5C5DD1B0D. C1 [Rightmyer, Molly G.] San Diego Nat Hist Museum, Biodivers Res Ctr Calif, San Diego, CA 92112 USA. [Rightmyer, Molly G.; Griswold, Terry] Utah State Univ, USDA ARS, Bee Biol & Systemat Lab, Logan, UT 84322 USA. [Rightmyer, Molly G.; Brady, Sean G.] Natl Museum Nat Hist, Dept Entomol, Washington, DC 20560 USA. RP Rightmyer, MG (reprint author), San Diego Nat Hist Museum, Biodivers Res Ctr Calif, POB 121390, San Diego, CA 92112 USA. EM molly_rightmyer@yahoo.com FU National Science Foundation [EF-0431330, DEB-0743542] FX We thank J. Ascher, S. Droege, S. Dumesh, E. Elle, L. Packer, T. Romankova, J. Rozen, Jr. and C. Sheffield for making specimens available for this study. We are additionally grateful to L. Packer and members of his lab for providing DNA barcoding data for many North American species sampled from TG's lab, which facilitated some of the new synonymies published herein. The Laboratories of Analytical Biology (National Museum of Natural History) provided molecular laboratory and computational infrastructure and Hong Zhao provided expert assistance in generating molecular data. We thank V. H. Gonzalez for sharing information on Anthidium palliventre, directing us to pertinent literature, and offering many useful comments on an early version of the manuscript. We are grateful to C. D. Michener, Christophe Praz, Lars Vilhelmsen and one anonymous reviewer for their insightful reviews of this manuscript. SGB acknowledges the National Science Foundation for research support under grants EF-0431330 and DEB-0743542. NR 44 TC 8 Z9 8 U1 2 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6970 J9 SYST ENTOMOL JI Syst. Entomol. PD JUL PY 2013 VL 38 IS 3 BP 561 EP 576 DI 10.1111/syen.12013 PG 16 WC Evolutionary Biology; Entomology SC Evolutionary Biology; Entomology GA 166MX UT WOS:000320560100008 ER PT J AU D'Amico, V Slavicek, J Podgwaite, JD Webb, R Fuester, R Peiffer, RA AF D'Amico, Vincent Slavicek, James Podgwaite, John D. Webb, Ralph Fuester, Roger Peiffer, Randall A. TI Deletion of v-chiA from a Baculovirus Reduces Horizontal Transmission in the Field SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID NUCLEAR POLYHEDROSIS-VIRUS; BOMBYX-MORI NUCLEOPOLYHEDROVIRUS; GYPSY-MOTH LEPIDOPTERA; AERIALLY-APPLIED GYPCHEK; LYMANTRIA-DISPAR; MOLECULAR-BIOLOGY; INSECT VIRUS; IN-VITRO; CHITINASE; GENE AB Nucleopolyhedroviruses (NPVs) can initiate devastating disease outbreaks in populations of defoliating Lepidoptera, a fact that has been exploited for the purposes of biological control of some pest insects. A key part of the horizontal transmission process of NPVs is the degradation of the larval integument by virus-coded proteins called chitinases, such as V-CHIA produced by the v-chiA genes. We used recombinant and naturally occurring strains of the Lymantria dispar NPV (LdMNPV) to test horizontal transmission in the field, release of virus from dead larvae under laboratory conditions, and cell lysis and virus release in cell culture. In the field, strains of LdMNPV lacking functional v-chiA genes showed reduced horizontal transmission compared to wild-type or repaired strains. These findings were mirrored by a marked reduction in released virus in laboratory tests and cell culture when the same strains were used to infect larvae or cells. Thus, this study tests the pivotal role of liquefaction and the v-chiA gene in field transmission for the first time and uses complementary laboratory data to provide a likely explanation for our findings. C1 [D'Amico, Vincent] USDA Forest Serv NRS 04, Dept Entomol & Wildlife Ecol, Newark, DE USA. [Slavicek, James] USDA Forest Serv NRS 04, Delaware, OH USA. [Podgwaite, John D.] USDA Forest Serv NRS 04, Hamden, CT USA. [Webb, Ralph] USDA ARS, Beltsville, MD USA. [Fuester, Roger] USDA ARS, Newark, DE USA. [Peiffer, Randall A.] Delaware State Univ, Dept Agr & Nat Resources, Dover, DE USA. RP D'Amico, V (reprint author), USDA Forest Serv NRS 04, Dept Entomol & Wildlife Ecol, Newark, DE USA. EM vincedamico@gmail.com FU U.S. Forest Service FX This work was funded by the U.S. Forest Service. NR 65 TC 5 Z9 5 U1 1 U2 8 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JUL PY 2013 VL 79 IS 13 BP 4056 EP 4064 DI 10.1128/AEM.00152-13 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA 158QB UT WOS:000319986200019 PM 23624474 ER PT J AU Kachergis, E Rocca, ME Fernandez-Gimenez, ME AF Kachergis, Emily Rocca, Monique E. Fernandez-Gimenez, Maria E. TI Comparison of species and trait-based approaches for describing sagebrush steppe response to range management SO APPLIED VEGETATION SCIENCE LA English DT Article DE Chemical shrub treatment; Community dynamics; Ecological site; Functional groups; Grazing; Monitoring; Northwestern Colorado; State-and-transition models ID ECOSYSTEM FUNCTION; TRANSITION MODELS; ALTERNATE STATES; PLANT TRAITS; VEGETATION; PRECIPITATION; COMMUNITIES; DISTURBANCE; FRAMEWORK; FIRE AB Question Is valuable information lost when plant trait group composition is used, rather than species composition, to describe plant community response to range management? Location Elkhead Watershed, Colorado, US. Methods Current model-building efforts use species composition to define changes in ecosystem state, but plant traits may offer a faster and more broadly applicable alternative. We (1) compare states defined by species composition to those defined by trait-based groups of differing complexity and (2) determine how management and environmental site characteristics relate to species- and trait group-defined states. We sampled 72 plots with different grazing and chemical shrub treatment histories on two soil types. We measured plant species composition in each plot and categorized species into trait groups using three classification schemes, which represented increasing numbers of traits and levels of classification complexity. The classifications employed easily measured traits that affect plant response to range management: life form, life history, resprouting ability, height, vegetative reproduction and N-fixation. Using hierarchical cluster analysis, we identified states with similar species or trait group composition. We explored relationships between each set of potential states and management history and environmental factors using logistic regression. Results Trait-based group composition and species composition identified many of the same potential states and responses to grazing and chemical shrub treatment. Relationships between species and trait group composition and management and environmental characteristics differed on the two soil types. Species composition was sensitive to more different management practices, on average, than trait group composition. Trait group composition revealed some relationships to management and environmental drivers that were not detected using species composition. Conclusion This study confirms that species composition is a more sensitive indicator of sagebrush steppe response to range management, and some information is lost with a trait-based approach. However, traits also add to depth of understanding by revealing additional community patterns related to different drivers. Using the most complex trait grouping scheme that is feasible in a particular study, and also looking for patterns based on simpler trait groups, will provide the most complete understanding of sagebrush steppe response to range management. C1 [Kachergis, Emily] ARS, Rangeland Resources Res Unit, USDA, Cheyenne, WY 82009 USA. [Rocca, Monique E.] Colorado State Univ, Dept Ecosyst Sci & Sustainabil, Ft Collins, CO 80523 USA. [Fernandez-Gimenez, Maria E.] Colorado State Univ, Dept Forest & Rangeland Stewardship, Ft Collins, CO 80523 USA. RP Kachergis, E (reprint author), ARS, Rangeland Resources Res Unit, USDA, 8408 Hildreth Rd, Cheyenne, WY 82009 USA. EM emily.kachergis@gmail.com; Monique.Rocca@colostate.edu; Maria.Fernandez-Gimenez@colostate.edu RI Rocca, Monique/N-4528-2013 OI Rocca, Monique/0000-0002-5298-1643 FU Colorado Agricultural Experiment Station [COL00698]; Natural Resource Conservation Service Colorado Conservation Innovation Grant [AG-8BO5-A-6-33]; US Department of Agriculture National Research Initiative Managed Ecosystems Program [COL0-2008-00725] FX We thank the coordinating editor and reviewers for valuable comments that improved the manuscript. Research was funded by the Colorado Agricultural Experiment Station (Project Number COL00698), a Natural Resource Conservation Service Colorado Conservation Innovation Grant (AG-8BO5-A-6-33), and a grant from the US Department of Agriculture National Research Initiative Managed Ecosystems Program (COL0-2008-00725). NR 44 TC 2 Z9 2 U1 3 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 J9 APPL VEG SCI JI Appl. Veg. Sci. PD JUL PY 2013 VL 16 IS 3 BP 355 EP 364 DI 10.1111/avsc.12004 PG 10 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 157GV UT WOS:000319885000002 ER PT J AU Miller, RS Sweeney, SJ AF Miller, R. S. Sweeney, S. J. TI Mycobacterium bovis (bovine tuberculosis) infection in North American wildlife: current status and opportunities for mitigation of risks of further infection in wildlife populations SO EPIDEMIOLOGY AND INFECTION LA English DT Review DE Adaptive disease management; Bovidae; bovine tuberculosis; Cervidae; Mycobacterium bovis; risk management; Suidae; wildlife ID WHITE-TAILED DEER; OPOSSUMS DIDELPHIS-VIRGINIANA; BUFFALO SYNCERUS CAFFER; KRUGER-NATIONAL-PARK; BOAR SUS-SCROFA; EXPERIMENTAL INOCULATION; ODOCOILEUS-VIRGINIANUS; MEDITERRANEAN ECOSYSTEM; DISEASE TRANSMISSION; ADAPTIVE MANAGEMENT AB Mycobacterium bovis (M. bovis), the causative agent of bovine tuberculosis, has been identified in nine geographically distinct wildlife populations in North America and Hawaii and is endemic in at least three populations, including members of the Bovidae, Cervidae, and Suidae families. The emergence of M. bovis in North American wildlife poses a serious and growing risk for livestock and human health and for the recreational hunting industry. Experience in many countries, including the USA and Canada, has shown that while M. bovis can be controlled when restricted to livestock species, it is almost impossible to eradicate once it has spread into ecosystems with free-ranging maintenance hosts. Therefore, preventing transmission of M. bovis to wildlife may be the most effective way to mitigate economic and health costs of this bacterial pathogen. Here we review the status of M. bovis infection in wildlife of North America and identify risks for its establishment in uninfected North American wildlife populations where eradication or control would be difficult and costly. We identified four common risk factors associated with establishment of M. bovis in uninfected wildlife populations in North America, (1) commingling of infected cattle with susceptible wildlife, (2) supplemental feeding of wildlife, (3) inadequate surveillance of at-risk wildlife, and (4) unrecognized emergence of alternate wildlife species as successful maintenance hosts. We then propose the use of integrated and adaptive disease management to mitigate these risk factors to prevent establishment of M. bovis in susceptible North American wildlife species. C1 [Miller, R. S.; Sweeney, S. J.] USDA, APHIS, Vet Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO USA. RP Miller, RS (reprint author), 2150 Ctr Ave,Bldg B, Ft Collins, CO 80526 USA. EM Ryan.S.Miller@aphis.usda.gov OI Miller, Ryan/0000-0003-3892-0251 NR 112 TC 23 Z9 23 U1 3 U2 30 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUL PY 2013 VL 141 IS 7 SI SI BP 1357 EP 1370 DI 10.1017/S0950268813000976 PG 14 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA 159GX UT WOS:000320035000003 PM 23657134 ER PT J AU Walter, WD Fischer, JW Anderson, CW Marks, DR Deliberto, T Robbe-Austerman, S Vercauteren, KC AF Walter, W. D. Fischer, J. W. Anderson, C. W. Marks, D. R. Deliberto, T. Robbe-Austerman, S. Vercauteren, K. C. TI Surveillance and movements of Virginia opossum (Didelphis virginiana) in the bovine tuberculosis region of Michigan SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Bovine tuberculosis (bTB); home range; indirect disease transmission; Mycobacterium bovis; resource selection analysis; surveillance ID WHITE-TAILED DEER; BADGER MELES-MELES; MYCOBACTERIUM-BOVIS; BRUSHTAIL POSSUMS; HOME-RANGE; ODOCOILEUS-VIRGINIANUS; POPULATION-DENSITY; RESOURCE SELECTION; TRUE PREVALENCE; TRANSMISSION AB Wildlife reservoir hosts of bovine tuberculosis (bTB) include Eurasian badgers (Meles meles) and brushtail possum (Trichosurus vulpecula) in the UK and New Zealand, respectively. Similar species warrant further investigation in the northern lower peninsula of Michigan, USA due to the continued presence of bTB on cattle farms. Most research in Michigan, USA has focused on interactions between white-tailed deer (Odocoileus virginianus) and cattle (Bos taurus) for the transmission of the infectious agent of bTB, Mycobacterium bovis, due to high deer densities and feeding practices. However, limited data are available on medium-sized mammals such as Virginia opossum (Didelphis virginiana; hereafter referred to as opossum) and their movements and home range in Michigan near cattle farms. We conducted surveillance of medium-sized mammals on previously depopulated cattle farms for presence of M. bovis infections and equipped opossum with Global Positioning System (GPS) technology to assess potential differences in home range between farms inside and outside the bTB core area that has had cattle test positive for M. bovis. On farms inside the bTB core area, prevalence in opossum was comparable [6%, 95% confidence interval (CI) 2.0-11.0] to prevalence in raccoon (Procyon lotor; 4%, 95% CI 1.0-9.0, P=0.439) whereas only a single opossum tested positive for M. bovis on farms outside the bTB core area. The prevalence in opossum occupying farms that had cattle test positive for M. bovis was higher (6.4%) than for opossum occupying farms that never had cattle test positive for M. bovis (0.9%, P=0.01). Mean size of home range for 50% and 95% estimates were similar by sex (P=0.791) both inside or outside the bTB core area (P=0.218). Although surveillance efforts and home range were not assessed on the same farms, opossum use of farms near structures was apparent as was selection for farms over surrounding forested habitats. The use of farms, stored feed, and structures by opossum, their ability to serve as vectors of M. bovis, and their propensity to ingest contaminated sources of M. bovis requires additional research in Michigan, USA. C1 [Walter, W. D.; Fischer, J. W.; Anderson, C. W.; Deliberto, T.; Vercauteren, K. C.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Marks, D. R.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Dis Program, Okemos, MI USA. [Robbe-Austerman, S.] Vet Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Vet Serv Labs, Ames, IA USA. RP Walter, WD (reprint author), Penn State Univ, US Geol Survey, Penn Cooperat Fish & Wildlife Res Unit, 403 Forest Resources Bldg, University Pk, PA 16802 USA. EM wdwalter@psu.edu FU United States Department of Agriculture, Animal Plant and Health Inspection Service, Wildlife Services and Veterinary Services, National Wildlife Research Center FX Funding was provided by the United States Department of Agriculture, Animal Plant and Health Inspection Service, Wildlife Services and Veterinary Services, National Wildlife Research Center. We thank Tony Aderman, Dane Williams and Rex Schenk for planning and assistance in surveillance and trapping efforts. We also thank the Michigan Department of Natural Resources for use of their Wildlife Disease Laboratory for conducting necropsies. NR 45 TC 2 Z9 2 U1 2 U2 22 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JUL PY 2013 VL 141 IS 7 SI SI BP 1498 EP 1508 DI 10.1017/S0950268813000629 PG 11 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA 159GX UT WOS:000320035000018 PM 23531427 ER PT J AU Funnell-Harris, DL Sattler, SE Pedersen, JF AF Funnell-Harris, Deanna L. Sattler, Scott E. Pedersen, Jeffrey F. TI Characterization of fluorescent Pseudomonas spp. associated with roots and soil of two sorghum genotypes SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE 2,4-diacetylphloroglucinol; Fusarium thapsinum; Plant genotype; Soil type; Rhizobacteria; Sorghum bicolor (L.) Moench ID 2,4-DIACETYLPHLOROGLUCINOL-PRODUCING PSEUDOMONAS; DIVERSITY; POPULATIONS; WHEAT; PHLD; RHIZOSPHERE; BACTERIA; MONOCULTURE; SEEDLINGS; STRAINS AB Sorghum is used as bioenergy feedstock, animal feed, and food. Economical methods for disease prevention and control are valuable for producers. Fluorescent Pseudomonas spp. were isolated from sorghum roots and surrounding soil with the goal of finding isolates that significantly inhibited sorghum fungal pathogens. Fluorescent pseudomonads were collected from seedlings of sorghum cultivars RTx433 and Redlan and wheat cultivar Lewjain, grown in two soils. Lewjain is known to support growth of producers of the antibiotic, 2,4-diacetylphloroglucinol (2,4-DAPG). Isolates from all three plants were assessed for hydrogen cyanide (HCN) and extracellular protease production, and for a 2,4-DAPG gene, phlD. Both soil type and plant type affected HCN- and protease-production, but phlD was not affected. Subsets of phlD (+) isolates were chosen to determine phlD genotypes and to conduct in vitro inhibition assays against sorghum pathogens. Most isolates from sorghum and wheat were genotype D, previously associated with superior root colonization. phlD (+) sorghum isolates were co-cultured with five sorghum pathogens. One isolate from each sorghum line exhibited inhibition to all five pathogens but more Redlan isolates were inhibitory to the virulent pathogen, Fusarium thapsinum, than RTx433 isolates. Nearly all inhibitory isolates from either sorghum cultivar were from one soil type. This is consistent with what had been previously observed in field studies: that soil type played a significant role in determining characteristics of fluorescent Pseudomonas spp. isolated from roots or soil, but sorghum genotype also had a considerable effect. C1 [Funnell-Harris, Deanna L.] Univ Nebraska, USDA ARS, Dept Plant Pathol, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. [Funnell-Harris, Deanna L.; Sattler, Scott E.; Pedersen, Jeffrey F.] Univ Nebraska, Lincoln, NE 68583 USA. [Sattler, Scott E.; Pedersen, Jeffrey F.] Univ Nebraska, USDA ARS, Dept Agron & Hort, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Funnell-Harris, DL (reprint author), Univ Nebraska, USDA ARS, Dept Plant Pathol, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. EM Deanna.Funnell-Harris@ars.usda.gov; Scott.Sattler@ars.usda.gov; jpedersen3@neb.rr.com NR 29 TC 4 Z9 4 U1 1 U2 14 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD JUL PY 2013 VL 136 IS 3 BP 469 EP 481 DI 10.1007/s10658-013-0179-6 PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA 155QR UT WOS:000319763600004 ER PT J AU Wald, MJ Considine, JM Turner, KT AF Wald, M. J. Considine, J. M. Turner, K. T. TI Determining the Elastic Modulus of Compliant Thin Films Supported on Substrates from Flat Punch Indentation Measurements SO EXPERIMENTAL MECHANICS LA English DT Article DE Indentation; Thin films; Finite elements; Substrate effects; Soft materials ID AXISYMMETRICAL INDENTATION; MECHANICAL-PROPERTIES; COATING/SUBSTRATE COMPOSITES; NANOINDENTATION; CONTACT AB Instrumented indentation is a technique that can be used to measure the elastic properties of soft thin films supported on stiffer substrates, including polymer films, cellulosic sheets, and thin layers of biological materials. When measuring thin film properties using indentation, the effect of the substrate must be considered. Most existing models for determining the properties of thin films from indentation measurements were developed for metal and dielectric films bonded to semiconductor substrates and have been applied to systems with film-substrate modulus ratios between 0.1 and 10. In the present work, flat punch indentation of a thin film either bonded to or in contact with a substrate is examined using finite element modeling. A broad range of film-substrate modulus ratios from 0.0001 to 1 are investigated. As the substrate is effectively rigid compared to the film when the film-substrate modulus ratio is less than 0.0001, the results are also useful for understanding systems with lower film-substrate modulus ratios. The effects of the contact radius, film thickness, elastic properties, and friction between the film and the substrate on the measured stiffness were quantified using finite element modeling in order to understand how the elastic properties of the film can be extracted from indentation measurements. A semi-analytical model was developed to describe the finite element modeling results and facilitate the use of the results to analyze experimental measurements. The model was validated through analysis of indentation measurements of thin polyethylene sheets that were supported on substrates of various stiffness. C1 [Wald, M. J.; Turner, K. T.] Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USA. [Considine, J. M.] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. RP Turner, KT (reprint author), Univ Penn, Dept Mech Engn & Appl Mech, Philadelphia, PA 19104 USA. EM kturner@seas.upenn.edu FU United States Postal Service; US Forest Service, Forest Products Laboratory FX We acknowledge financial support from the United States Postal Service and the US Forest Service, Forest Products Laboratory. NR 20 TC 2 Z9 2 U1 3 U2 26 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0014-4851 J9 EXP MECH JI Exp. Mech. PD JUL PY 2013 VL 53 IS 6 BP 931 EP 941 DI 10.1007/s11340-012-9705-2 PG 11 WC Materials Science, Multidisciplinary; Mechanics; Materials Science, Characterization & Testing SC Materials Science; Mechanics GA 163JU UT WOS:000320332900004 ER PT J AU Anderson-Teixeira, KJ Miller, AD Mohan, JE Hudiburg, TW Duval, BD DeLucia, EH AF Anderson-Teixeira, Kristina J. Miller, Adam D. Mohan, Jacqueline E. Hudiburg, Tara W. Duval, Benjamin D. DeLucia, Evan H. TI Altered dynamics of forest recovery under a changing climate SO GLOBAL CHANGE BIOLOGY LA English DT Review DE biogeochemistry; climate feedback; FACE; irrigation; regime shift; succession; throughfall manipulation; warming ID ALTERNATIVE STABLE STATES; ATMOSPHERIC CARBON-DIOXIDE; SCRUB-OAK ECOSYSTEM; DIVERSITY-DISTURBANCE RELATIONSHIPS; NET PRIMARY PRODUCTIVITY; PONDEROSA PINE FORESTS; FINE-ROOT RESPONSES; CO2 ENRICHMENT FACE; BOREAL BLACK SPRUCE; OLD-GROWTH FORESTS AB Forest regeneration following disturbance is a key ecological process, influencing forest structure and function, species assemblages, and ecosystem-climate interactions. Climate change may alter forest recovery dynamics or even prevent recovery, triggering feedbacks to the climate system, altering regional biodiversity, and affecting the ecosystem services provided by forests. Multiple lines of evidence - including global-scale patterns in forest recovery dynamics; forest responses to experimental manipulation of CO2, temperature, and precipitation; forest responses to the climate change that has already occurred; ecological theory; and ecosystem and earth system models - all indicate that the dynamics of forest recovery are sensitive to climate. However, synthetic understanding of how atmospheric CO2 and climate shape trajectories of forest recovery is lacking. Here, we review these separate lines of evidence, which together demonstrate that the dynamics of forest recovery are being impacted by increasing atmospheric CO2 and changing climate. Rates of forest recovery generally increase with CO2, temperature, and water availability. Drought reduces growth and live biomass in forests of all ages, having a particularly strong effect on seedling recruitment and survival. Responses of individual trees and whole-forest ecosystems to CO2 and climate manipulations often vary by age, implying that forests of different ages will respond differently to climate change. Furthermore, species within a community typically exhibit differential responses to CO2 and climate, and altered community dynamics can have important consequences for ecosystem function. Age- and species-dependent responses provide a mechanism by which climate change may push some forests past critical thresholds such that they fail to recover to their previous state following disturbance. Altered dynamics of forest recovery will result in positive and negative feedbacks to climate change. Future research on this topic and corresponding improvements to earth system models will be a key to understanding the future of forests and their feedbacks to the climate system. C1 [Anderson-Teixeira, Kristina J.] Natl Zool Pk, Smithsonian Conservat Biol Inst, Conservat Ecol Ctr, Front Royal, VA 22630 USA. [Anderson-Teixeira, Kristina J.] Smithsonian Trop Res Inst, Smithsonian Inst Global Earth Observ, Ctr Trop Forest Sci, Panama City, Panama. [Miller, Adam D.; DeLucia, Evan H.] Univ Illinois, Inst Genom Biol, Chicago, IL 60680 USA. [Mohan, Jacqueline E.] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Hudiburg, Tara W.; DeLucia, Evan H.] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. [Hudiburg, Tara W.; DeLucia, Evan H.] Univ Illinois, Urbana, IL 61801 USA. [Duval, Benjamin D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Anderson-Teixeira, KJ (reprint author), Natl Zool Pk, Smithsonian Conservat Biol Inst, Natl Zool Pk,1500 Remount Rd, Front Royal, VA 22630 USA. EM TeixeiraK@si.edu FU DOE [DE-SC0008085] FX Thanks to Jonathan Thompson and Sean McMahon for helpful discussion and to three anonymous reviewers for helpful comments. This research was supported by DOE grant #DE-SC0008085 to KJAT, EHD, and BDD. NR 224 TC 46 Z9 46 U1 21 U2 321 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2013 VL 19 IS 7 BP 2001 EP 2021 DI 10.1111/gcb.12194 PG 21 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 158IT UT WOS:000319963500004 PM 23529980 ER PT J AU Prasad, AM Gardiner, JD Iverson, LR Matthews, SN Peters, M AF Prasad, Anantha M. Gardiner, Judith D. Iverson, Louis R. Matthews, Stephen N. Peters, Matthew TI Exploring tree species colonization potentials using a spatially explicit simulation model: implications for four oaks under climate change SO GLOBAL CHANGE BIOLOGY LA English DT Article DE cell-based simulation model; convolution; eastern United States; fast fourier transforms; long-distance dispersal; oak forest types; range-limit dynamics; tree species migration ID PLANT PHENOTYPIC PLASTICITY; LONG-DISTANCE DISPERSAL; EASTERN UNITED-STATES; SEED DISPERSAL; POSTGLACIAL DISPERSAL; VEGETATION RESPONSE; MIGRATION CAPACITY; LATE QUATERNARY; HABITAT MODELS; RANGE MARGINS AB Climate change impacts tree species differentially by exerting unique pressures and altering their suitable habitats. We previously predicted these changes in suitable habitat for current and future climates using a species habitat model (DISTRIB) in the eastern United States. Based on the accuracy of the model, the species assemblages should eventually reflect the new quasi-equilibrium suitable habitats (similar to 2100) after accounting for the lag in colonization. However, it is an open question if and when these newly suitable habitats will be colonized under current fragmented landscapes and realistic migration rates. To evaluate this, we used a spatially explicit cell-based model (SHIFT) that estimates colonization potentials under current fragmented habitats and several estimates of historical migration rates at a 1km resolution. Computation time, which was previously the biggest constraint, was overcome by a novel application of convolution and Fast Fourier Transforms. SHIFT outputs, when intersected with future suitable habitats predicted by DISTRIB, allow assessment of colonization potential under future climates. In this article, we show how our approach can be used to screen multiple tree species for their colonization potentials under climate change. In particular, we use the DISTRIB and SHIFT models in combination to assess if the future dominant forest types in the north will really be dominated by oaks, as modelled via DISTRIB. Even under optimistic scenarios, we conclude that only a small fraction of the suitable habitats of oaks predicted by DISTRIB is likely to be occupied within 100years, and this will be concentrated in the first 10-20km from the current boundary. We also show how DISTRIB and SHIFT can be used to evaluate the potential for assisted migration of vulnerable tree species, and discuss the dynamics of colonization at range limits. C1 [Prasad, Anantha M.; Iverson, Louis R.; Matthews, Stephen N.; Peters, Matthew] US Forest Serv, No Res Stn, USDA, Delaware, OH 43015 USA. [Gardiner, Judith D.] Ohio Supercomp Ctr, Columbus, OH 43212 USA. [Matthews, Stephen N.] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. RP Prasad, AM (reprint author), US Forest Serv, No Res Stn, USDA, 359 Main Rd, Delaware, OH 43015 USA. EM aprasad@fs.fed.us RI Matthews, Stephen/D-1050-2012; Iverson, Louis/C-7554-2009 OI Iverson, Louis/0000-0001-9501-471X FU Northern Research Station Global Change Program, USDA Forest Service FX The authors thank the Northern Research Station Global Change Program, USDA Forest Service, for funding this project and Mark Schwartz, Vijay Gadepally and Harrison Smith for reviewing the manuscript. The authors declare no conflict of interest to the best of their knowledge. NR 74 TC 13 Z9 13 U1 3 U2 70 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JUL PY 2013 VL 19 IS 7 BP 2196 EP 2208 DI 10.1111/gcb.12204 PG 13 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 158IT UT WOS:000319963500018 PM 23526802 ER PT J AU Miresmailli, S Zeri, M Zangerl, AR Bernacchi, CJ Berenbaum, MR DeLucia, EH AF Miresmailli, Saber Zeri, Marcelo Zangerl, Arthur R. Bernacchi, Carl J. Berenbaum, May R. DeLucia, Evan H. TI Impacts of herbaceous bioenergy crops on atmospheric volatile organic composition and potential consequences for global climate change SO GLOBAL CHANGE BIOLOGY BIOENERGY LA English DT Article DE atmospheric chemistry; bioenergy; BVOC; global change ID BIOGENIC VOC EMISSIONS; ISOPRENE EMISSION; PROFILE RELATIONSHIPS; COMPOUND EMISSIONS; MASS-SPECTROMETRY; PLANT VOLATILES; BIOFUEL CROPS; FLUXES; CARBON; METHANOL AB The introduction of new crops to agroecosystems can change the chemical composition of the atmosphere by altering the amount and type of plant-derived biogenic volatile organic compounds (BVOCs). BVOCs are produced by plants to aid in defense, pollination, and communication. Once released into the atmosphere, they have the ability to influence its chemical and physical properties. In this study, we compared BVOC emissions from three potential bioenergy crops and estimated their theoretical impacts on bioenergy agroecosystems. The crops chosen were miscanthus (Miscanthusxgiganteus), switchgrass (Panicum virgatum), and an assemblage of prairie species (mix of 28 species). The concentration of BVOCs was different within and above plant canopies. All crops produced higher levels of emissions at the upper canopy level. Miscanthus produced lower amounts of volatiles compared with other grasses. The chemical composition of volatiles differed significantly among plant communities. BVOCs from miscanthus were depleted in terpenoids relative to the other vegetation types. The carbon flux via BVOC emissions, calculated using the flux-gradient method, was significantly higher in the prairie assemblage compared with miscanthus and switchgrass. The BVOC carbon flux was approximately three orders of magnitude lower than the net fluxes of carbon measured over the same fields using eddy covariance systems. Extrapolation of our findings to the landscape scale leads us to suggest that the widespread adoption of bioenergy crops could potentially alter the composition of BVOCs in the atmosphere, thereby influencing its warming potential, the formation of atmospheric particulates, and interactions between plants and arthropods. Our data and projections indicate that, among at least these three potential options for bioenergy production, miscanthus is likely to have lower impacts on atmospheric chemistry and biotic interactions mediated by these volatiles when miscanthus is planted on the landscape scale. C1 [Miresmailli, Saber; Zeri, Marcelo; Berenbaum, May R.; DeLucia, Evan H.] Univ Illinois, Energy BioSci Inst, Urbana, IL USA. [Zeri, Marcelo] Inst Nacl Pesquisas Espaciais, Ctr Ciencia Sistema Terrestre, Sao Paulo, IL, Brazil. [Zangerl, Arthur R.; Berenbaum, May R.] Univ Illinois, Dept Entomol, Urbana, IL USA. [Miresmailli, Saber] Sumatics LLC, New York, NY USA. [Bernacchi, Carl J.; DeLucia, Evan H.] Univ Illinois, Dept Plant Biol, Urbana, IL USA. [Bernacchi, Carl J.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. RP DeLucia, EH (reprint author), Univ Illinois, Energy BioSci Inst, Urbana, IL USA. EM deLucia@illinois.edu FU Energy Biosciences Institute FX The Energy Biosciences Institute funded this research. The authors thank Alan Yanahan, Allen Lawrance, Robert Orpet, Jacob Becraft, and Olivia Niziolek for their assistance with data collection. NR 74 TC 3 Z9 3 U1 4 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1757-1693 EI 1757-1707 J9 GCB BIOENERGY JI GCB Bioenergy PD JUL PY 2013 VL 5 IS 4 BP 375 EP 383 DI 10.1111/j.1757-1707.2012.01189.x PG 9 WC Agronomy; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA 158CX UT WOS:000319947300004 ER PT J AU Chen, SE Florax, RJ Snyder, SD AF Chen, Susan Elizabeth Florax, Raymond J. Snyder, Samantha D. TI Obesity and fast food in urban markets: a new approach using geo-referenced micro data SO HEALTH ECONOMICS LA English DT Article DE obesity; fast food; spatial econometrics ID ATHEROSCLEROSIS RISK; SOCIAL INTERACTIONS; ECONOMIC-ANALYSIS; RESTAURANTS; LOCATION; AVAILABILITY; COMMUNITIES; RESIDENTS; DYNAMICS; BEHAVIOR AB This paper presents a new method of assessing the relationship between features of the built environment and obesity, particularly in urban areas. Our empirical application combines georeferenced data on the location of fast-food restaurants with data about personal health, behavioral, and neighborhood characteristics. We define a local food environment' for every individual utilizing buffers around a person's home address. Individual food landscapes are potentially endogenous because of spatial sorting of the population and food outlets, and the body mass index (BMI) values for individuals living close to each other are likely to be spatially correlated because of observed and unobserved individual and neighborhood effects. The potential biases associated with endogeneity and spatial correlation are handled using spatial econometric estimation techniques. Our application provides quantitative estimates of the effect of proximity to fast-food restaurants on obesity in an urban food market. We also present estimates of a policy simulation that focuses on reducing the density of fast-food restaurants in urban areas. In the simulations, we account for spatial heterogeneity in both the policy instruments and individual neighborhoods and find a small effect for the hypothesized relationships between individual BMI values and the density of fast-food restaurants. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Chen, Susan Elizabeth] Univ Alabama, Tuscaloosa, AL 35487 USA. [Florax, Raymond J.] Purdue Univ, W Lafayette, IN 47907 USA. [Snyder, Samantha D.] USDA, ERS, Washington, DC 20250 USA. RP Chen, SE (reprint author), Univ Alabama, 269 Alston Hall Box 870224, Tuscaloosa, AL 35487 USA. EM sechen@cba.ua.edu OI Chen, Susan/0000-0003-4409-7417 NR 35 TC 4 Z9 4 U1 5 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1057-9230 J9 HEALTH ECON JI Health Econ. PD JUL PY 2013 VL 22 IS 7 BP 835 EP 856 DI 10.1002/hec.2863 PG 22 WC Economics; Health Care Sciences & Services; Health Policy & Services SC Business & Economics; Health Care Sciences & Services GA 160JL UT WOS:000320115700006 PM 22911977 ER PT J AU Sanford, MR Olson, JK Lewis, WJ Tomberlin, JK AF Sanford, M. R. Olson, J. K. Lewis, W. J. Tomberlin, J. K. TI The Effect of Sucrose Concentration on Olfactory-Based Associative Learning in Culex quinquefasciatus Say (Diptera: Culicidae) SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Reward strength; classical conditioning; binary logistic regression; mosquito sugar-feeding ID MEMORIZED HOME-RANGE; PROBOSCIS EXTENSION; MALARIA VECTORS; APIS-MELLIFERA; MOSQUITOS; POLLINATION; ATTRACTION; BLOSSOMS; PLANTS; ODORS AB A classical conditioning approach was used to examine the ability of Culex quinquefasciatus Say (Diptera: Culicidae) to associate odor with a sugar-meal of varying quality. The objectives of this study were to investigate the impact of different sucrose concentrations (5 %, 10 %, or 50 %) on positive response to conditioning and to examine sucrose concentration preference following exposure to a 10 % solution. Mosquitoes conditioned in conjunction with all three sucrose concentrations showed evidence of learning; including the concentration of the conditioning stimulus, and the sex of the mosquito. Using colored solutions to determine feeding patterns of experienced mosquitoes indicated male mosquitoes showed no preference but females showed a preference for 10 % over 5 % sucrose but not between 10 % and 50 % sucrose solutions. C1 [Sanford, M. R.; Olson, J. K.; Tomberlin, J. K.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Lewis, W. J.] USDA ARS, Crop Management Res Unit, Tifton, GA 31793 USA. RP Sanford, MR (reprint author), Univ Calif Davis, Sch Vet Med, Dept Pathol Microbiol & Immunol, 1 Shields Ave, Davis, CA 95616 USA. EM uranotaenia@gmail.com RI Sanford, Michelle/C-9575-2011 OI Sanford, Michelle/0000-0001-6212-4023 FU Texas Mosquito Control Association; Texas AM University FX We wish to thank Sandy Allan and Erin Vrzal of the Center for Medical, Agricultural, and Veterinary Entomology, USDA-ARS, Gainesville, FL, USA for providing us with mosquitoes, Thoris Greene and Glen Rains of the Department of Biological and Agricultural Engineering, University of Georgia, Tifton Campus, GA, USA for their technical assistance, Roberto Gorena of the Mosquito Control Division of the Harris County Public Health and Environmental Services, Houston, TX, USA for statistical advice and useful comments on an earlier draft of this manuscript. We also thank Mark Johnsen for his useful discussions and comments, which greatly improved this manuscript. The James Gus Foyle Memorial Scholarship from the Texas Mosquito Control Association and the Regents Fellowship from Texas A&M University provided funding support. This study was conducted in partial fulfillment of a doctoral degree to M. R. S. NR 43 TC 4 Z9 4 U1 1 U2 20 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD JUL PY 2013 VL 26 IS 4 BP 494 EP 513 DI 10.1007/s10905-012-9368-y PG 20 WC Entomology SC Entomology GA 159KY UT WOS:000320045500003 ER PT J AU Royo, AA Ristau, TE AF Royo, Alejandro A. Ristau, Todd E. TI Stochastic and deterministic processes regulate spatio-temporal variation in seed bank diversity SO JOURNAL OF VEGETATION SCIENCE LA English DT Article DE - and -diversity; assembly; disturbance; seed longevity; temperate forests ID TEMPERATE DECIDUOUS FOREST; LIFE-HISTORY TRAITS; SPECIES COMPOSITION; HERBACEOUS LAYER; SECONDARY SUCCESSION; ALLEGHENY HARDWOODS; NATURAL COMMUNITIES; NESTEDNESS ANALYSIS; BETA DIVERSITY; OLD-GROWTH AB Question Seed banks often serve as reservoirs of taxonomic and genetic diversity that buffer plant populations and influence post-disturbance vegetation trajectories; yet evaluating their importance requires understanding how their composition varies within and across spatial and temporal scales (- and -diversity). Shifts in seed bank diversity are strongly governed by the deterministic role of differential seed longevities. Nevertheless, spatio-temporal variability in propagule depletion from and recruitment into seed banks may alter both - and -diversity. Here, we ask to what degree deterministic seed exhaustion, stochasticity in recruitment and mortality, or both, shape - and -diversity? Location Temperate hardwood forest stands of varying ages in northern Pennsylvania, USA. Methods We surveyed the seed bank and herbaceous vegetation communities at 39 sites and examined whether the species richness, abundance and composition of either community differs among and within sites of increasing age ranging from 43 to 106yr old. We explored how -diversity (species richness) and abundance (percentage cover, seed density) varied across the chronosequence age using regression analyses. We analysed differences in -diversity (community composition) using permutational multivariate analyses (i.e. PERMANOVA, PERMDISP). Finally, we tested whether community composition of the herbaceous layer and seed bank communities exhibited nestedness, where nestedness refers to the degree to which less species-rich sites are a non-random subset of more species-rich sites. Results We found seed bank -diversity and abundance consistently declined across a gradient of increasingly older sites. Moreover, nestedness analyses indicated species composition at older sites represented a subset of the species found at younger sites characterized by species with persistent seeds. Nevertheless, seed bank communities demonstrated divergent compositional trajectories, whereby older sites were increasingly dissimilar, not only from younger sites, but also from each other. Conclusions Our results suggest that even in assemblages strongly structured by deterministic forces, such as seed banks, minor stochastic differences in colonization and extinction events may increase -diversity over time. Therefore, we argue deterministic and stochastic processes are complementary factors governing post-disturbance turnover in species assemblages, and suggest that seed banks contribute to species persistence and overall forest community diversity across space and time. C1 [Royo, Alejandro A.; Ristau, Todd E.] US Forest Serv, USDA, No Res Stn, Irvine, PA 16329 USA. RP Royo, AA (reprint author), US Forest Serv, USDA, No Res Stn, POB 267, Irvine, PA 16329 USA. EM aroyo@fs.fed.us; tristau@fs.fed.us FU Pennsylvania Department of Conservation and Natural Resources, Bureau of Forestry; U.S. Department of Agriculture, Forest Service Northern Research Station FX This work was supported by the Pennsylvania Department of Conservation and Natural Resources, Bureau of Forestry and the U.S. Department of Agriculture, Forest Service Northern Research Station. We thank Martin Dovciak, Jan Plue, Matthew D. Trager, Mark Vellend and three anonymous reviewers for comments on this manuscript, and J. Stanovick for statistical consultation and review. NR 83 TC 8 Z9 8 U1 9 U2 91 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1100-9233 J9 J VEG SCI JI J. Veg. Sci. PD JUL PY 2013 VL 24 IS 4 BP 724 EP 734 DI 10.1111/jvs.12011 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA 160JB UT WOS:000320114600014 ER PT J AU Caesar-TonThat, TC Espeland, E Caesar, AJ Sainju, UM Lartey, RT Gaskin, JF AF Caesar-TonThat, The Can Espeland, Erin Caesar, Anthony J. Sainju, Upendra M. Lartey, Robert T. Gaskin, John F. TI Effects of Agaricus lilaceps Fairy Rings on Soil Aggregation and Microbial Community Structure in Relation to Growth Stimulation of Western Wheatgrass (Pascopyrum smithii) in Eastern Montana Rangeland SO MICROBIAL ECOLOGY LA English DT Article ID CELL-SURFACE PROPERTIES; FATTY-ACID ANALYSIS; FLUORESCENT PSEUDOMONAD; BINDING-PROPERTIES; CONTINUOUS COTTON; ENZYME-ACTIVITIES; C SEQUESTRATION; ORGANIC-MATTER; SPRING WHEAT; MODEL SYSTEM AB Stimulation of plant productivity caused by Agaricus fairy rings has been reported, but little is known about the effects of these fungi on soil aggregation and the microbial community structure, particularly the communities that can bind soil particles. We studied three concentric zones of Agaricus lilaceps fairy rings in Eastern Montana that stimulate western wheatgrass (Pascopyrum smithii): outside the ring (OUT), inside the ring (IN), and stimulated zone adjacent to the fungal fruiting bodies (SZ) to determine (1) soil aggregate proportion and stability, (2) the microbial community composition and the N-acetyl-beta-d-glucosaminidase activity associated with bulk soil at 0-15 cm depth, (3) the predominant culturable bacterial communities that can bind to soil adhering to wheatgrass roots, and (4) the stimulation of wheatgrass production. In bulk soil, macroaggregates (4.75-2.00 and 2.00-0.25 mm) and aggregate stability increased in SZ compared to IN and OUT. The high ratio of fungal to bacteria (fatty acid methyl ester) and N-acetyl-beta-d-glucosaminidase activity in SZ compared to IN and OUT suggest high fungal biomass. A soil sedimentation assay performed on the predominant isolates from root-adhering soil indicated more soil-binding bacteria in SZ than IN and OUT; Pseudomonas fluorescens and Stenotrophomonas maltophilia isolates predominated in SZ, whereas Bacillus spp. isolates predominated in IN and OUT. This study suggests that growth stimulation of wheatgrass in A. lilaceps fairy rings may be attributed to the activity of the fungus by enhancing soil aggregation of bulk soil at 0-15 cm depth and influencing the amount and functionality of specific predominant microbial communities in the wheatgrass root-adhering soil. C1 [Caesar-TonThat, The Can; Espeland, Erin; Caesar, Anthony J.; Sainju, Upendra M.; Lartey, Robert T.; Gaskin, John F.] ARS, USDA, Sidney, MT 59270 USA. RP Caesar-TonThat, TC (reprint author), ARS, USDA, 1500 North Cent Ave, Sidney, MT 59270 USA. EM thecan.caesar@ars.usda.gov OI Espeland, Erin/0000-0001-8541-3610 FU Bureau of Land Management (Montana, South and North Dakota) FX The authors express their sincere appreciation to L. L. Solberg, M. O'Mara, and K. Mann for technical assistance. We also acknowledge Dr. J. Jabro (USDA-ARS, Sidney, MT) and Dr. Lora B. Perkins (South Dakota State University) for their constructive comments on the manuscript. This study was supported by the Bureau of Land Management (Montana, South and North Dakota). NR 66 TC 5 Z9 5 U1 2 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0095-3628 J9 MICROB ECOL JI Microb. Ecol. PD JUL PY 2013 VL 66 IS 1 BP 120 EP 131 DI 10.1007/s00248-013-0194-3 PG 12 WC Ecology; Marine & Freshwater Biology; Microbiology SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Microbiology GA 159LX UT WOS:000320048000013 PM 23455430 ER PT J AU Gasulla, F Jain, R Barreno, E Guera, A Balbuena, TS Thelen, JJ Oliver, MJ AF Gasulla, Franscico Jain, Renuka Barreno, Eva Guera, Alfredo Balbuena, Tiago S. Thelen, Jay J. Oliver, Melvin J. TI The response of Asterochloris erici (Ahmadjian) Skaloud et Peksa to desiccation: a proteomic approach SO PLANT CELL AND ENVIRONMENT LA English DT Article DE Asterochloris; desiccation tolerance; drying rate; Hsp90; lichen; protome; -tubulin ID PLANT CRATEROSTIGMA-PLANTAGINEUM; TORTULA-RURALIS GAMETOPHYTES; GRASS SPOROBOLUS-STAPFIANUS; CHLOROPHYLL-A FLUORESCENCE; HEAT-SHOCK PROTEINS; RESURRECTION PLANT; WATER RELATIONS; SELAGINELLA-LEPIDOPHYLLA; XEROPHYTA-HUMILIS; MASS-SPECTROMETRY AB The study of desiccation tolerance of lichens, and of their chlorobionts in particular, has frequently focused on the antioxidant system that protects the cell against photo-oxidative stress during dehydration/rehydration cycles. In this study, we used proteomic and transcript analyses to assess the changes associated with desiccation in the isolated phycobiont Asterochloris erici. Algae were dried either slowly (5-6h) or rapidly (<60min), and rehydrated after 24h in the desiccated state. To identify proteins that accumulated during the drying or rehydration processes, we employed two-dimensional (2D) difference gel electrophoresis (DIGE) coupled with individual protein identification using trypsin digestion and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Proteomic analyses revealed that desiccation caused an increase in relative abundance of only 11-13 proteins, regardless of drying rate, involved in glycolysis, cellular protection, cytoskeleton, cell cycle, and targeting and degradation. Transcripts of five Hsp90 and two -tubulin genes accumulated primarily at the end of the dehydration process. In addition, transmission electron microscopy (TEM) images indicate that ultrastructural cell injuries, perhaps resulting from physical or mechanical stress rather than metabolic damage, were more intense after rapid dehydration. This occurred with no major change in the proteome. These results suggest that desiccation tolerance of A.erici is achieved by constitutive mechanisms. C1 [Gasulla, Franscico; Barreno, Eva] Univ Valencia, Dpt Bot, ICBiBE, E-46100 Burjassot, Spain. [Jain, Renuka; Balbuena, Tiago S.; Thelen, Jay J.] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA. [Guera, Alfredo] Univ Alcala de Henares, Dpto Biol Vegetal, Alcala De Henares 28871, Spain. [Oliver, Melvin J.] ARS, USDA, PGRU, Columbia, MO 65211 USA. RP Oliver, MJ (reprint author), ARS, USDA, PGRU, Columbia, MO 65211 USA. EM mel.oliver@ars.usda.gov RI Gasulla, Francisco/K-3742-2014; Barreno Rodriguez, Eva/M-2525-2014; Guera, Alfredo/H-7576-2015 OI Gasulla, Francisco/0000-0002-4957-4078; Barreno Rodriguez, Eva/0000-0002-2622-4550; Guera, Alfredo/0000-0003-0028-5491 FU Spanish Ministry of Science and Innovation [CGL2009-13429-C02-01/02]; Generalitat Valenciana [PROMETEO 174/2008, CGL2012-40058-C02-01/02]; USDA-APRS (USA) [3622-21000-034] FX This study was supported in part by the Spanish Ministry of Science and Innovation (CGL2009-13429-C02-01/02, FEDER) and the Generalitat Valenciana (PROMETEO 174/2008 and CGL2012-40058-C02-01/02) and the USDA-APRS (CRIS Project 3622-21000-034, USA). We would like to thank Drs F. Gracia-Breijo and J. Reig-Armi ana (Jardi Botanic, University of Valencia) for their help in TEM analysis of structural changes. We are grateful to the Central Support Service in Experimental Research (SCSIE), University of Valencia and UPV (Spain) for providing TEM, and to Jim Elder (USDA-ARS-PGRU, MO, USA) for his technical assistance in the genomic work. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the United States Department of Agriculture, and does not imply its approval to the exclusion of other products that may also be suitable. NR 92 TC 13 Z9 13 U1 3 U2 37 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD JUL PY 2013 VL 36 IS 7 BP 1363 EP 1378 DI 10.1111/pce.12065 PG 16 WC Plant Sciences SC Plant Sciences GA 157DD UT WOS:000319875100011 PM 23305100 ER PT J AU Bendix, C Mendoza, JM Stanley, DN Meeley, R Harmon, FG AF Bendix, Claire Mendoza, Juan M. Stanley, Desiree N. Meeley, Robert Harmon, Frank G. TI The circadian clock-associated gene gigantea1 affects maize developmental transitions SO PLANT CELL AND ENVIRONMENT LA English DT Article DE circadian clock; conz1; development; flowering time; gigantea; growth; maize; phase change; photoperiod; zcn8 ID PSEUDO-RESPONSE-REGULATOR; QUANTITATIVE TRAIT LOCUS; ARABIDOPSIS-THALIANA; FLOWERING-TIME; DISTINCT ROLES; LONG-DISTANCE; PHOTOPERIOD SENSITIVITY; TARGET GENES; BLUE-LIGHT; T PROTEIN AB The circadian clock is an internal timing mechanism that allows plants to make developmental decisions in accordance with environmental conditions. In model plants, circadian clock-associated gigantea (gi) genes are directly involved in control of growth and developmental transitions. The maize gigantea1 (gi1) gene is the more highly expressed of the two gi homeologs, and its function is uncharacterized. To understand the role of gi1 in the regulatory networks of the maize circadian clock system, gi1 mutants were evaluated for changes in flowering time, phase change and growth control. When grown in long-day (LD) photoperiods, gi1 mutants flowered earlier than non-mutant plants, but this difference was not apparent in short-day (SD) photoperiods. Therefore, gi1 participates in a pathway that suppresses flowering in LD photoperiods, but not in SD. Part of the underlying cause of early flowering was up-regulated expression of the FT-like floral activator gene zea mays centroradialis8 (zcn8) and the CONSTANS-like flowering regulatory gene constans of zea mays1 (conz1). gi1 mutants also underwent vegetative phase change earlier and grew taller than non-mutant plants. These findings indicate gi1 has a repressive function in multiple regulatory pathways that govern maize growth and development. C1 [Bendix, Claire; Stanley, Desiree N.; Harmon, Frank G.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Mendoza, Juan M.; Harmon, Frank G.] ARS, Plant Gene Express Ctr, USDA, Albany, CA 94710 USA. [Meeley, Robert] Pioneer Hibred, Crop Genet Res, Johnston, IA 50130 USA. RP Harmon, FG (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. EM fharmon@berkeley.edu OI Bendix, Claire/0000-0002-4723-8652 FU United States Department of Agriculture [335-21000-026-00D] FX We thank Drs. Sarah Hake and George Chuck at the Plant Gene Expression Center (Albany, CA) for their generosity with advice and materials. Jeffrey Omotoy, Carolyn Duong, Dean Natwick and Sam Hayward provided invaluable technical assistance. This work is supported by the United States Department of Agriculture [335-21000-026-00D to F.G.H.]. NR 86 TC 9 Z9 11 U1 1 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD JUL PY 2013 VL 36 IS 7 BP 1379 EP 1390 DI 10.1111/pce.12067 PG 12 WC Plant Sciences SC Plant Sciences GA 157DD UT WOS:000319875100012 PM 23336247 ER PT J AU Celik, A Altikat, S Way, TR AF Celik, Ahmet Altikat, Sefa Way, Thomas R. TI Strip tillage width effects on sunflower seed emergence and yield SO SOIL & TILLAGE RESEARCH LA English DT Article DE Conservation tillage; No-tillage; Strip tillage; Strip width; Sunflower ID SOIL PHYSICAL-PROPERTIES; CONSERVATION TILLAGE; ROOT-GROWTH; CLAY SOILS; WATER; WHEAT; CORN; NITROGEN; SYSTEMS; BARLEY AB Strip tillage is a conservation practice in which narrow strips, generally totaling less than 50% of the field area, are tilled. We hypothesized that strip tillage would be beneficial for long-term soil quality improvement, erosion control, and environmental protection because it also protects crop residues so they can cover and continuously protect the soil surface. A two-year field experiment with three replicates was conducted to quantify effects of three strip widths on selected soil physical properties, seed emergence and yield of sunflower (Helianthus annuus). A powered row crop rotary hoe which is a group of narrow rotary tillers spaced evenly along the width of the toolbar and powered by the tractor power take-off was used to till soil in strips. The rotary hoe was equipped with C-type blades and was used to till strip widths of 37.5, 30 and 22.5 cm by changing the blade position and number of flanges on each row of the rotary hoe. A constant rotor rotational speed (370 rpm), forward tractor speed (5.4 km h(-1)) and tillage depth (10 cm) were used to create the three strip widths that corresponded to tilled zones encompassing 50, 40, and 30% of the field area, respectively. A pneumatic seeder with 75 cm row intervals was used for planting. The results show that as strip width increased, soil temperature increased but soil moisture content decreased due to evaporation loss from the tilled surface of the strips. Sunflower seed emergence ranged from 67 to 93%, with the lowest levels occurring with 22.5 cm strips. Plant length and stalk diameter also increased as strip width increased. Seed yields for the two years also increased with strip width, averaging 4.4, 4.1, and 3.9 Mg ha(-1) for the 37.5, 30 and 22.5 cm strip widths, respectively. Based on these results, although seed yield was least for the 22.5 cm strip width, tractor fuel efficiency was greatest for that width and the soil tended to retain more moisture for that width, compared to the 30 and 37.5 cm widths, so the 22.5 cm strip width is recommended to the eastern Turkey. (C) 2013 Elsevier B.V. All rights reserved. C1 [Celik, Ahmet] Ataturk Univ, Fac Agr, Dept Agr Machinery, TR-25240 Erzurum, Turkey. [Altikat, Sefa] Igdir Univ, Fac Agr, Dept Biosyst Engn, TR-76000 Igdir, Turkey. [Way, Thomas R.] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL 36832 USA. RP Celik, A (reprint author), Ataturk Univ, Fac Agr, Dept Agr Machinery, TR-25240 Erzurum, Turkey. EM ahcelik@atauni.edu.tr FU Scientific Research Administration Unit of Ataturk University, Erzurum, Turkey FX This research was supported by the Scientific Research Administration Unit of Ataturk University, Erzurum, Turkey. NR 42 TC 4 Z9 5 U1 3 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 J9 SOIL TILL RES JI Soil Tillage Res. PD JUL PY 2013 VL 131 BP 20 EP 27 DI 10.1016/j.still.2013.03.004 PG 8 WC Soil Science SC Agriculture GA 164NO UT WOS:000320417000003 ER PT J AU Aziz, I Mahmood, T Islam, KR AF Aziz, Irfan Mahmood, Tariq Islam, K. Rafiq TI Effect of long term no-till and conventional tillage practices on soil quality SO SOIL & TILLAGE RESEARCH LA English DT Article DE Microbial biomass; Basal respiration; Total carbon and nitrogen; Aggregate stability; Particulate organic matter; Inductive additive approach; Tillage ID MICROBIAL BIOMASS CARBON; ORGANIC-MATTER DYNAMICS; MANAGEMENT; NITROGEN; INDICATORS; SEQUESTRATION; FRACTIONS; IMPACTS; SYSTEMS; POOLS AB Management systems influence soil quality over time. A randomized block design in 2 (tillage system) x 3 (crop rotation) factorial arrangement was laid-out to evaluate the impact of tillage and crop rotation (2002-2007) on soil quality. Conventional tillage and No-till were factored into continuous corn, corn-soybean, and corn-soybean-wheat-Cowpea systems. Ten soil cores were collected at 0-7.5, 7.5-15, 15-22.5 and 22.5-30 cm depths and analyzed for biological, chemical and physical parameters. The inductive additive approach was used to calculate biological, chemical, physical and composite soil quality indices. A significant impact of no tillage on different physical chemical and biological parameters was observed. The estimated soil quality index was significantly higher in soil under No-till than conventional tillage. Soil biological quality is a sensitive and consistent indicator of soil quality in response to management practices. (C) 2013 Elsevier B.V. All rights reserved. C1 [Aziz, Irfan] PMAS Arid Agr Univ, Dept Agron, Rawalpindi 46300, Pakistan. [Mahmood, Tariq] PMAS Arid Agr Univ, Dept Environm Sci, Rawalpindi 46300, Pakistan. [Islam, K. Rafiq] Ohio State Univ South Ctr, Piketon, OH USA. [Islam, K. Rafiq] USDA ARS, Columbus, OH USA. RP Aziz, I (reprint author), PMAS Arid Agr Univ, Dept Agron, Rawalpindi 46300, Pakistan. EM irfaz15@yahoo.com NR 53 TC 31 Z9 34 U1 9 U2 121 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-1987 EI 1879-3444 J9 SOIL TILL RES JI Soil Tillage Res. PD JUL PY 2013 VL 131 BP 28 EP 35 DI 10.1016/j.still.2013.03.002 PG 8 WC Soil Science SC Agriculture GA 164NO UT WOS:000320417000004 ER PT J AU White, EM Latta, G Alig, RJ Skog, KE Adams, DM AF White, Eric M. Latta, Greg Alig, Ralph J. Skog, Kenneth E. Adams, Darius M. TI Biomass production from the US forest and agriculture sectors in support of a renewable electricity standard SO ENERGY POLICY LA English DT Article DE Renewable electricity standards; Forest and agriculture; Land exchange ID UNITED-STATES; BIOENERGY PRODUCTION; LOGGING RESIDUES; ECONOMIC-IMPACTS; ENERGY; MARKETS; CROPS; SUSTAINABILITY; SWITCHGRASS; GRASS AB Production of renewable energy from biomass has been promoted as means to improve greenhouse gas balance in energy production, improve energy security, and provide jobs and income. However, uncertainties remain as to how the agriculture and forest sectors might jointly respond to increased demand for bioelectricity feedstocks and the potential environmental consequences of increased biomass production. We use an economic model to examine how the agriculture and forest sectors might combine to respond to increased demands for bioelectricity under simulated future nationallevel renewable electricity standards. Both sectors are projected to contribute biomass, although energy crops, like switchgrass, produced on agriculture land are projected to be the primary feedstocks. At the highest targets for bioelectricity production, we project increased conversion of forest to agriculture land in support of agriculture biomass production. Although land conversion takes place in response to renewable electricity mandates, we project only minor increases in forest and agriculture emissions. Similarly, crop prices were projected to generally be stable in the face of increased bioelectricity demand and displacement of traditional agriculture crops. (C) 2013 Elsevier Ltd. All rights reserved. C1 [White, Eric M.; Latta, Greg; Adams, Darius M.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Alig, Ralph J.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Skog, Kenneth E.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP White, EM (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, 280 Peavy Hall, Corvallis, OR 97331 USA. EM eric.white@oregonstate.edu NR 43 TC 14 Z9 14 U1 2 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0301-4215 J9 ENERG POLICY JI Energy Policy PD JUL PY 2013 VL 58 BP 64 EP 74 DI 10.1016/j.enpol.2013.02.029 PG 11 WC Energy & Fuels; Environmental Sciences; Environmental Studies SC Energy & Fuels; Environmental Sciences & Ecology GA 147OV UT WOS:000319177800008 ER PT J AU Valentine, HT Amateis, RL Gove, JH Makela, A AF Valentine, Harry T. Amateis, Ralph L. Gove, Jeffrey H. Makela, Annikki TI Crown-rise and crown-length dynamics: application to loblolly pine SO FORESTRY LA English DT Article ID GROWTH; TREES; SPRUCE; STAND; MODEL AB The original crown-rise model estimates the average height of a crown-base in an even-aged mono-species stand of trees. We have elaborated this model to reduce bias and prediction error, and to also provide crown-base estimates for individual trees. Results for the latter agree with a theory of branch death based on resource availability and allocation. We use the improved model to estimate the growth and stem-profile development of a mean-tree in an even-aged stand of loblolly pine. Predictions show good agreement with data from a loblolly pine spacing trial. C1 [Valentine, Harry T.; Gove, Jeffrey H.] US Forest Serv, Durham, NH 03824 USA. [Amateis, Ralph L.] VPI & SU, Coll Nat Resources & Environm, Blacksburg, VA 24061 USA. [Makela, Annikki] Univ Helsinki, Dept Forest Sci, FIN-00014 Helsinki, Finland. RP Valentine, HT (reprint author), US Forest Serv, 271 Mast Rd, Durham, NH 03824 USA. EM hvalentine@fs.fed.us OI Makela, Annikki/0000-0001-9633-7350 NR 18 TC 4 Z9 6 U1 1 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X J9 FORESTRY JI Forestry PD JUL PY 2013 VL 86 IS 3 BP 371 EP 375 DI 10.1093/forestry/cpt007 PG 5 WC Forestry SC Forestry GA 142RK UT WOS:000318814600008 ER PT J AU Westbrook, JW Resende, MFR Munoz, P Walker, AR Wegrzyn, JL Nelson, CD Neale, DB Kirst, M Huber, DA Gezan, SA Peter, GF Davis, JM AF Westbrook, Jared W. Resende, Marcio F. R., Jr. Munoz, Patricio Walker, Alejandro R. Wegrzyn, Jill L. Nelson, C. Dana Neale, David B. Kirst, Matias Huber, Dudley A. Gezan, Salvador A. Peter, Gary F. Davis, John M. TI Association genetics of oleoresin flow in loblolly pine: discovering genes and predicting phenotype for improved resistance to bark beetles and bioenergy potential SO NEW PHYTOLOGIST LA English DT Article DE association genetics; genomic selection; genotypexenvironment interactions; herbivory defense; Pinus; terpenoid ID WESTERN UNITED-STATES; RESIN DUCT FORMATION; SPRUCE PICEA-ABIES; GENOMIC SELECTION; TAEDA L.; TERPENOID BIOSYNTHESIS; DIPHOSPHATE SYNTHASES; ARABIDOPSIS-THALIANA; CONIFER DEFENSE; CLIMATE-CHANGE AB Rapidly enhancing oleoresin production in conifer stems through genomic selection and genetic engineering may increase resistance to bark beetles and terpenoid yield for liquid biofuels. We integrated association genetic and genomic prediction analyses of oleoresin flow (g24h-1) using 4854 single nucleotide polymorphisms (SNPs) in expressed genes within a pedigreed population of loblolly pine (Pinus taeda) that was clonally replicated at three sites in the southeastern United States. Additive genetic variation in oleoresin flow (h2 approximate to 0.12-0.30) was strongly correlated between years in which precipitation varied (ra approximate to 0.95), while the genetic correlation between sites declined from 0.8 to 0.37 with increasing differences in soil and climate among sites. A total of 231 SNPs were significantly associated with oleoresin flow, of which 81% were specific to individual sites. SNPs in sequences similar to ethylene signaling proteins, ABC transporters, and diterpenoid hydroxylases were associated with oleoresin flow across sites. Despite this complex genetic architecture, we developed a genomic prediction model to accelerate breeding for enhanced oleoresin flow that is robust to environmental variation. Results imply that breeding could increase oleoresin flow 1.5- to 2.4-fold in one generation. C1 [Westbrook, Jared W.; Resende, Marcio F. R., Jr.; Munoz, Patricio; Walker, Alejandro R.; Kirst, Matias; Peter, Gary F.; Davis, John M.] Univ Florida, Genet Inst, Forest Genom Lab, Gainesville, FL 32611 USA. [Walker, Alejandro R.; Kirst, Matias; Huber, Dudley A.; Gezan, Salvador A.; Peter, Gary F.; Davis, John M.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Wegrzyn, Jill L.; Neale, David B.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Nelson, C. Dana] US Forest Serv, Southern Inst Forest Genet, USDA, Southern Res Stn, Saucier, MS 39574 USA. RP Davis, JM (reprint author), Univ Florida, Genet Inst, Forest Genom Lab, 1376 Mowry Rd,Rm 320, Gainesville, FL 32611 USA. EM jmdavis@ufl.edu OI Wegrzyn, Jill/0000-0001-5923-0888 FU The Forest Biology Research Cooperative; National Science Foundation Plant Genome Research Program [0501763]; United States Department of Agriculture CSREES Food and Agricultural Sciences National Needs Graduate Fellowship FX The Forest Biology Research Cooperative managed the CCLONES population and funded oleoresin collection. SNP genotyping was funded by the National Science Foundation Plant Genome Research Program award no. 0501763. Special thanks are due to L.H. Lott and J.H. Roberds (Southern Institute of Forest Genetics) for oleoresin taps; members of the University of Florida Forest Genomics Laboratory, especially J. Zhang, for assistance with oleoresin collection; M. Chavez Monte-Alegre for weighing the oleoresin tubes; and C. Dervinis and K. Smith for assistance with the BLAST analyses. J.W.W. was supported by a United States Department of Agriculture CSREES Food and Agricultural Sciences National Needs Graduate Fellowship. NR 78 TC 14 Z9 16 U1 2 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X J9 NEW PHYTOL JI New Phytol. PD JUL PY 2013 VL 199 IS 1 BP 89 EP 100 DI 10.1111/nph.12240 PG 12 WC Plant Sciences SC Plant Sciences GA 152GR UT WOS:000319520200012 PM 23534834 ER PT J AU McDonald, MC Oliver, RP Friesen, TL Brunner, PC McDonald, BA AF McDonald, Megan C. Oliver, Richard P. Friesen, Timothy L. Brunner, Patrick C. McDonald, Bruce A. TI Global diversity and distribution of three necrotrophic effectors in Phaeosphaeria nodorum and related species SO NEW PHYTOLOGIST LA English DT Article DE co-evolution; fungal effectors; NB-LRR; necrotrophic effectors; Phaeosphaeria nodorum; plant pathogen; population genetics ID POPULATION GENETIC-ANALYSIS; STAGONOSPORA-NODORUM; COMPUTER-PROGRAM; FUNGAL; WHEAT; VIRULENCE; EVOLUTION; PROTEINS; PATHOGENICITY; SPECIFICITY AB Population genetic and phylogenetic studies have shown that Phaeosphaeria nodorum is a member of a species complex that probably shares its center of origin with wheat (Triticum aestivum and Triticum durum). We examined the evolutionary histories of three known necrotrophic effectors (NEs) produced by P. nodorum and compared them with neutral loci. We screened over 1000 individuals for the presence/absence of each effector and assigned each individual to a multi-effector genotype. Diversity at each NE locus was assessed by sequencing c. 200 individuals for each locus. We found significant differences in effector frequency among populations. We propose that these differences reflect the presence/absence of the corresponding susceptibility gene in wheat cultivars. The population harboring the highest sequence diversity was different for each effector locus and never coincided with populations harboring the highest diversity at neutral loci. Coalescent and phylogenetic analyses showed a discontinuous presence of all three NEs among nine closely related Phaeosphaeria species. Only two of the nine species were found to harbor NEs. We present evidence that the three described NEs of P.nodorum were transmitted to its sister species, Phaeosphaeria avenaria tritici 1, via interspecific hybridization. C1 [McDonald, Megan C.; Brunner, Patrick C.; McDonald, Bruce A.] ETH, Inst Integrat Biol, Plant Pathol Grp, CH-8092 Zurich, Switzerland. [Oliver, Richard P.] Curtin Univ Technol, Dept Environm & Agr, Australian Ctr Necrotroph Fungal Pathogens, Perth, WA 6845, Australia. [Friesen, Timothy L.] ARS, USDA, No Crop Sci Lab, Cereal Crops Res Unit, Fargo, ND 58105 USA. RP McDonald, MC (reprint author), ETH, Inst Integrat Biol, Plant Pathol Grp, CH-8092 Zurich, Switzerland. EM megan.mcdonald@anu.edu.au RI McDonald, Bruce/A-2748-2008; McDonald, Megan/O-7662-2014 OI McDonald, Bruce/0000-0002-5332-2172; McDonald, Megan/0000-0002-9129-7393 FU ETH Zurich; Australian Grains Research and Development Corporation; USDA-NIFA AFRI Microbial Biology Program-Competitive grant [2009-04265] FX The authors acknowledge the Genetic Diversity Center at ETH Zurich for providing sequencing facilities and the ETH Zurich for funding this research. We also thank the Australian Grains Research and Development Corporation and the USDA-NIFA AFRI Microbial Biology Program-Competitive grant no. 2009-04265 for additional funding. NR 50 TC 20 Z9 20 U1 2 U2 41 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JUL PY 2013 VL 199 IS 1 BP 241 EP 251 DI 10.1111/nph.12257 PG 11 WC Plant Sciences SC Plant Sciences GA 152GR UT WOS:000319520200024 PM 23550706 ER PT J AU Feng, M Sexton, JO Huang, CQ Masek, JG Vermote, EF Gao, F Narasimhan, R Channan, S Wolfe, RE Townshend, JR AF Feng, Min Sexton, Joseph O. Huang, Chengquan Masek, Jeffrey G. Vermote, Eric F. Gao, Feng Narasimhan, Raghuram Channan, Saurabh Wolfe, Robert E. Townshend, John R. TI Global surface reflectance products from Landsat: Assessment using coincident MODIS observations SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat; Surface reflectance; Global; MODIS; Quality assessment ID LEAF-AREA-INDEX; ATMOSPHERIC CORRECTION; BOREAL FOREST; ABSORBED PAR; EOS-MODIS; ETM+ DATA; COVER; CLASSIFICATION; PERFORMANCE; METHODOLOGY AB Global, long-term monitoring of changes in Earth's land surface requires quantitative comparisons of satellite images acquired under widely varying atmospheric conditions. Although physically based estimates of surface reflectance (SR) ultimately provide the most accurate representation of Earth's surface properties, there has never been a globally consistent SR dataset at the spatial resolution (<1 ha) or temporal extent (similar to 40 years) of the Landsat mission. To increase the consistency and robustness of Landsat-based land cover monitoring, we atmospherically corrected the Global Land Survey (GLS) Landsat dataset using the Landsat Ecosystem Disturbance Adaptive Processing System (LEDAPS) implementation of the Second Simulation of the Satellite Signal in the Solar Spectrum ( 6S) radiative transfer model. The GLS provides synoptic, orthorectified, cloud-free Landsat coverage of Earth's land area in four nominal epochs (1975, 1990, 2000, and 2005). This paper presents the resulting GLS surface reflectance dataset and a global assessment of the 2000- and 2005-epoch data against coincident Moderate Resolution Imaging Spectroradiometer (MODIS) daily SR and Normalized Bidirectional Distribution Function-Adjusted Reflectance (NBAR) measurements. Agreement with respect to MODIS SR and NBAR data is very high, with overall discrepancies (Root-Mean-Squared Deviation (RMSD)) between 1.3 and 2.8 percent reflectance for Landsat-7 Enhanced Thematic Mapper Plus (ELM +) and between 2.2 and 3.5 percent reflectance for Landsat-5 Thematic Mapper (TM). The resulting Landsat surface reflectance dataset and the associated quality metrics for each image are hosted on the Global Land Cover Facility web site for free download (http://www.landcover.org/data/gls_SR). This new repository will provide consistent, calibrated, multi-decadal image data for robust land cover change detection and monitoring across the Earth sciences. (C) 2013 Elsevier Inc. All rights reserved. C1 [Feng, Min; Sexton, Joseph O.; Huang, Chengquan; Narasimhan, Raghuram; Channan, Saurabh; Townshend, John R.] Univ Maryland, Dept Geog, Global Land Cover Facil, College Pk, MD 20742 USA. [Masek, Jeffrey G.] NASA, Biospher Sci Branch, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Vermote, Eric F.; Wolfe, Robert E.] NASA, Terr Phys Lab, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Gao, Feng] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. RP Feng, M (reprint author), Univ Maryland, Dept Geog, Global Land Cover Facil, College Pk, MD 20742 USA. EM feng.tank@gmail.com RI Masek, Jeffrey/D-7673-2012; Wolfe, Robert/E-1485-2012; OI Wolfe, Robert/0000-0002-0915-1855; Huang, Chengquan/0000-0003-0055-9798 FU NASA [NNH06ZDA001N-MEASURES, NNH07ZDA001N-LCLUC, NNH06ZDA001N-EOS] FX Funding support for this study was provided by the following NASA programs: Making Earth System Data Records for Use in Research Environments (NNH06ZDA001N-MEASURES), Land Cover and Land Use Change (NNH07ZDA001N-LCLUC), and Earth System Science Research Using Data and Products from Terra, Aqua, and Acrimsat Satellites (NNH06ZDA001N-EOS). The MODIS daily products used in this study were obtained from Greg Ederer and Robert Wolfe (NASA Goddard Space Flight Center). The GLS datasets were sent to GLCF by Rachel Headley (USGS). We also acknowledge the three anonymous reviewers for their helpful comments on earlier versions of the manuscript. NR 50 TC 34 Z9 35 U1 3 U2 49 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUL PY 2013 VL 134 BP 276 EP 293 DI 10.1016/j.rse.2013.02.031 PG 18 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 148GS UT WOS:000319233200021 ER PT J AU Schmidt, JA Washburn, BE Devault, TL Seamans, TW AF Schmidt, Jason A. Washburn, Brian E. Devault, Travis L. Seamans, Thomas W. TI Do Native Warm-season Grasslands Near Airports Increase Bird Strike Hazards? SO AMERICAN MIDLAND NATURALIST LA English DT Article ID CONSERVATION RESERVE PROGRAM; VEGETATION-HEIGHT MANAGEMENT; WILDLIFE RESPONSES; SITE SELECTION; HABITAT-AREA; COMMUNITIES; DENSITY; PRAIRIE; FIELDS; VOLES AB Bird aircraft collisions (bird strikes) are a recognized safety hazard and land uses that attract birds hazardous to aircraft should be avoided on and near airports. Many airfields contain large areas of anthropogenic grassland habitats, often dominated by cool season grasses. Land managed as native warm season grasses (NWSG) potentially could increase bird strike hazards on and near airports by attracting hazardous birds and harboring small mammals that are prey for hazardous raptors. We investigated bird and small mammal communities at three NWSG areas and three adjacent on airfield grassland areas in western Ohio, U. S. A. to determine whether NWSG increased bird strike hazards. Species specific differences in bird abundance and density were evident between the two landcover types, presumably the result of differences in plant community characteristics. Seven species of birds were found exclusively in NWSG or airfield grasslands. Birds of species categorized as 'moderate' to 'extremely high' in regard to hazard (severity) level to aircraft accounted for only 6% and 2% of all birds observed in airfield grasslands and NWSG areas, respectively. Small mammal capture success was approximately three times higher in NWSG areas, although raptor abundance did not differ between the two landcover types. Our findings suggest that NWSG might be considered a viable land use adjacent to airfields; however, similar research at additional locations, including larger NWSG areas, should be conducted. C1 [Schmidt, Jason A.; Washburn, Brian E.; Devault, Travis L.; Seamans, Thomas W.] USDA Wildlife Serv, Natl Wildlife Res Ctr, Sandusky, OH 44870 USA. RP Washburn, BE (reprint author), USDA Wildlife Serv, Natl Wildlife Res Ctr, 6100 Columbus Ave, Sandusky, OH 44870 USA. EM brian.e.washburn@aphis.usda.gov FU Federal Aviation Administration (FAA); U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center; U.S. Air Force; National Park Service; city of Toledo; city of Dayton; Toledo Area Metroparks; Aullwood Audubon Center and Farm FX We thank the Federal Aviation Administration (FAA) and the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center for funding and support. Opinions expressed in this study do not necessarily reflect current FAA policy decisions governing the control of wildlife on or near airports. In particular, we thank J. Pelc, A. Montoney, J. Cepek, and D. Helon for help with various aspects of this study. In addition, the U.S. Air Force (R. Alcantara and W. Kassinos), the National Park Service (J. Helton), the cities of Toledo (D. Spaugy and D. Podiak) and Dayton (G. Turner and W. Krygowski), Toledo Area Metroparks (K. Menard), and Aullwood Audubon Center and Farm (C. Krueger) provided study site access and/or support. We thank L. Tyson and D. Steyer for their field support and two reviewers for their efforts to improve this manuscript. The National Wildlife Research Center Institutional Animal Care and Use Committee approved all procedures involving birds and small mammals (QA-1710). NR 54 TC 2 Z9 3 U1 9 U2 24 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 EI 1938-4238 J9 AM MIDL NAT JI Am. Midl. Nat. PD JUL PY 2013 VL 170 IS 1 BP 144 EP 157 DI 10.1674/0003-0031-170.1.144 PG 14 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AK1WH UT WOS:000338208500011 ER PT J AU Witherup, C Ragone, D Wiesner-Hanks, T Irish, B Scheffler, B Simpson, S Zee, F Zuberi, MI Zerega, NJC AF Witherup, Colby Ragone, Diane Wiesner-Hanks, Tyr Irish, Brian Scheffler, Brian Simpson, Sheron Zee, Francis Zuberi, M. Iqbal Zerega, Nyree J. C. TI DEVELOPMENT OF MICROSATELLITE LOCI IN ARTOCARPUS ALTILIS (MORACEAE) AND CROSS-AMPLIFICATION IN CONGENERIC SPECIES SO APPLICATIONS IN PLANT SCIENCES LA English DT Article DE Artocarpus altilis; Artocarpus camansi; Artocarpus heterophyllus; Artocarpus mariannensis; breadfruit; jackfruit; Moraceae ID BREADFRUIT ARTOCARPUS; GENETIC DIVERSITY AB Premise of the study: Microsatellite loci were isolated and characterized from enriched genomic libraries of Artocarpus altilis (breadfruit) and tested in four Artocarpus species and one hybrid. The microsatellite markers provide new tools for further studies in Artocarpus. Methods and Results: A total of 25 microsatellite loci were evaluated across four Artocarpus species and one hybrid. Twenty-one microsatellite loci were evaluated on A. altilis (241), A. camansi (34), A. mariannensis (15), and A. altilis x mariannensis (64) samples. Nine of those loci plus four additional loci were evaluated on A. heterophyllus (jackfruit, 426) samples. All loci are polymorphic for at least one species. The average number of alleles ranges from two to nine within taxa. Conclusions: These microsatellite primers will facilitate further studies on the genetic structure and evolutionary and domestication history of Artocarpus species. They will aid in cultivar identification and establishing germplasm conservation strategies for breadfruit and jackfruit. C1 [Witherup, Colby; Zerega, Nyree J. C.] Chicago Bot Garden, Dept Plant Sci, Glencoe, IL 60022 USA. [Witherup, Colby; Wiesner-Hanks, Tyr; Zerega, Nyree J. C.] Northwestern Univ, Evanston, IL 60208 USA. [Ragone, Diane] Natl Trop Bot Garden, Breadfruit Inst, Kalaheo, HI 96741 USA. [Irish, Brian] USDA, Trop Agr Res Stn, Mayaguez, PR 00680 USA. [Scheffler, Brian; Simpson, Sheron] USDA, ARS, Genom & Bioinformat Res Unit, Stoneville, MS 38776 USA. [Zee, Francis] USDA, Trop Plant Genet Resources & Dis Res, PBARC, Hilo, HI 96720 USA. [Zuberi, M. Iqbal] Rajshahi Univ, Rajshahi 6205, Bangladesh. RP Zerega, NJC (reprint author), Chicago Bot Garden, Dept Plant Sci, Glencoe, IL 60022 USA. EM nzerega@chicagobotanic.org FU National Science Foundation [NSF DEB REVSYS 0919119]; Plant Biology and Conservation Award from Northwestern University; American Society of Plant Taxonomists Graduate Student Research Grant; U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) National Programs FX The authors thank S. Hussein and I. Cole for assistance with specimen collection, J. Fant and D. Reitz for assistance in the laboratory, and J. Fant and three anonymous reviewers for thoughtful comments on the manuscript. This study was funded in part by the National Science Foundation (NSF DEB REVSYS 0919119), a Plant Biology and Conservation Award from Northwestern University, an American Society of Plant Taxonomists Graduate Student Research Grant, and U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) National Programs. NR 12 TC 5 Z9 5 U1 0 U2 6 PU BOTANICAL SOC AMER INC PI ST LOUIS PA PO BOX 299, ST LOUIS, MO 63166-0299 USA SN 2168-0450 J9 APPL PLANT SCI JI Appl. Plant Sci. PD JUL PY 2013 VL 1 IS 7 AR 1200423 DI 10.3732/apps.1200423 PG 6 WC Plant Sciences SC Plant Sciences GA AQ1QT UT WOS:000342556800006 ER PT J AU Cho, S Fossler, CP Diez-Gonzalez, F Wells, SJ Hedberg, CW Kaneene, JB Ruegg, PL Warnick, LD Bender, JB AF Cho, Seongbeom Fossler, Charles P. Diez-Gonzalez, Francisco Wells, Scott J. Hedberg, Craig W. Kaneene, John B. Ruegg, Pamela L. Warnick, Lorin D. Bender, Jeffrey B. TI Herd-level risk factors associated with fecal shedding of Shiga toxin-encoding bacteria on dairy farms in Minnesota, USA SO CANADIAN VETERINARY JOURNAL-REVUE VETERINAIRE CANADIENNE LA English DT Article AB This study aimed to identify herd-level risk factors associated with fecal shedding of Shiga toxin-encoding bacteria (STB) on dairy cattle farms in Minnesota, USA. After adjustment for farm size, risk factors included: use of total mixed ration (TMR) for lactating dairy cows [odds ratio (OR) = 3.0; 95% confidence interval (CI): 1.8 to 5.1], no use of monensin for weaned calves (OR = 4.8, 95% CI: 2.5, 9.3), and no use of decoquinate for preweaned calves (OR = 2.2, 95% CI: 1.4, 3.6). Fecal shedding of STB was more common in small herds (< 100 cows, OR = 3.6, 95% CI: 2.1, 6.2) than in large herds (>= 100 cows). Herd management factors related to cattle feeding practices were associated with fecal shedding of STB. C1 [Cho, Seongbeom] Seoul Natl Univ, Coll Vet Med, Dept Vet Publ Hlth, Seoul 151742, South Korea. [Cho, Seongbeom] Seoul Natl Univ, Coll Vet Med, Res Inst Vet Sci, Seoul 151742, South Korea. [Fossler, Charles P.] USDA APHIS VS, Ctr Epidemiol, Ft Collins, CO 80526 USA. [Fossler, Charles P.] USDA APHIS VS, Ctr Anim Hlth, Ft Collins, CO 80526 USA. [Diez-Gonzalez, Francisco] Univ Minnesota, Dept Food Sci & Nutr, Minneapolis, MN 55108 USA. [Wells, Scott J.; Bender, Jeffrey B.] Univ Minnesota, Dept Vet Populat Med, Minneapolis, MN 55108 USA. [Hedberg, Craig W.] Univ Minnesota, Dept Environm Hlth Sci, Minneapolis, MN 55108 USA. [Kaneene, John B.] Michigan State Univ, Populat Med Center, E Lansing, MI 48824 USA. [Ruegg, Pamela L.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Warnick, Lorin D.] Cornell Univ, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA. RP Cho, S (reprint author), Seoul Natl Univ, Coll Vet Med, Dept Vet Publ Hlth, Seoul 151742, South Korea. EM chose@snu.ac.kr FU Academic Health Center Faculty Research and Department Program (FRD), University of Minnesota [00-07]; USDA/CSREES National Research Initiative [99-35212-8563] FX This study was supported by a grant from the Academic Health Center Faculty Research and Department Program (FRD #00-07), University of Minnesota, and USDA/CSREES National Research Initiative (Epidemiological Approaches of Food Safety, award No. 99-35212-8563). NR 17 TC 1 Z9 1 U1 0 U2 0 PU CANADIAN VET MED ASSOC PI OTTAWA PA 339 BOOTH ST, OTTAWA, ONTARIO K1R 7K1, CANADA SN 0008-5286 EI 1928-9022 J9 CAN VET J JI Can. Vet. J.-Rev. Vet. Can. PD JUL PY 2013 VL 54 IS 7 BP 693 EP 697 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA V36PU UT WOS:000209224300009 PM 24155466 ER PT J AU Aramouni, F Herald, T Abu Ghoush, M AF Aramouni, F. Herald, T. Abu Ghoush, M. TI Development of a non-commercial sugar-free barbecue sauce SO EMIRATES JOURNAL OF FOOD AND AGRICULTURE LA English DT Article DE Sugar free; Barbecue Sauce; Quality Evaluation ID OBESITY; COST; XANTHAN; GUM AB There has always been a challenge in manufacturing a sugar free sauce. A basic barbecue sauce formulation was used to make 5 sugar-free preparations combining selected levels of xanthan gum, modified waxy maize starch, sucralose, and acesulfame-K. Physical, chemical, microbial and sensory properties were used to evaluate the product quality. Total Aerobic Plate Count was below detectable limits before and after the incubation period for all 6 products. Total Soluble Solids, water activity and pH -before and after incubation- of the control were significantly different from all 5 sugar-free treatments. Descriptive sensory analysis of prepared products showed that SU treatment (2.0% Starch+ 0.3% Sucralose) exhibited the best in all the sensory properties that were determined compared to all other treatments. One sugar free preparation was significantly more viscous than the control. This study will help producers in formulating a new a sugar free healthy barbecue sauce with properties closely comparable to a sugar containing control formula. C1 [Aramouni, F.] Kansas State Univ, Inst Food Sci, Dept Anim Sci & Ind, Manhattan, KS 66506 USA. [Herald, T.] USDA ARS, Ctr Grain, Manhattan, KS 66502 USA. [Herald, T.] USDA ARS, Anim Hlth Res Ctr, Manhattan, KS 66502 USA. [Abu Ghoush, M.] King Faisal Univ, Dept Food & Nutr Sci, Al Hufuf, Saudi Arabia. RP Abu Ghoush, M (reprint author), King Faisal Univ, Dept Food & Nutr Sci, Al Hufuf, Saudi Arabia. EM mabughoush@kfu.edu.sa NR 24 TC 0 Z9 0 U1 1 U2 7 PU UNITED ARAB EMIRATES UNIV PI AL AIN PA P. O. BOX 17551, AL AIN, U ARAB EMIRATES SN 2079-052X EI 2079-0538 J9 EMIR J FOOD AGR JI Emir. J. Food Agric. PD JUL PY 2013 VL 25 IS 7 BP 509 EP 515 DI 10.9755/ejfa.v25i7.14466 PG 7 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA AS7PK UT WOS:000344447400003 ER PT J AU Henry, TJ Johnson, CA AF Henry, Thomas J. Johnson, Christine A. TI RANDALL TOBIAS SCHUH: A CELEBRATION OF HIS CAREER AND ACCOMPLISHMENTS ON THE OCCASION OF HIS 70TH BIRTHDAY SO ENTOMOLOGICA AMERICANA LA English DT Article C1 [Henry, Thomas J.] ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, Washington, DC 20013 USA. [Johnson, Christine A.] Amer Museum Nat Hist, Div Invertebrate Zool, New York, NY 10024 USA. RP Henry, TJ (reprint author), ARS, Systemat Entomol Lab, USDA, Natl Museum Nat Hist,Smithsonian Inst, MRC-168, Washington, DC 20013 USA. EM thomas.henry@ars.usda.gov; cjohnson@amnh.org FU American Museum of Natural History FX We are grateful to the many authors who contributed to this Festschrift volume, especially those who wrote personal tributes, including Toby's wife Brenda Massie, his daughter Ella Massie-Schuh, Gerry Cassis (University of New South Wales, Sydney), Katrina Menard (Sam Noble Museum, University of Oklahoma, Norman), and Ruth Salas (The American Museum of American History, New York). We also thank The New York Entomological Society for supporting this special volume and The American Museum of Natural History for funding; without this support this publication would not have been possible. NR 96 TC 0 Z9 0 U1 0 U2 0 PU NEW YORK ENTOMOLOGICAL SOC INC PI NEW YORK PA C/O AMER MUSEUM NAT HIST 79TH & CENTRAL PARK WEST, NEW YORK, NY 10024 USA SN 1947-5136 EI 1947-5144 J9 ENTOMOL AM-NY JI Entomol. Am. PD JUL PY 2013 VL 119 IS 1-2 BP 1 EP 6 DI 10.1664/13-SN-005.1 PG 6 WC Entomology SC Entomology GA V35YA UT WOS:000209179800001 ER PT J AU Hesler, LS AF Hesler, Louis S. TI RISK TO NATIVE UROLEUCON APHIDS (HEMIPTERA: APHIDIDAE) FROM NON-NATIVE LADY BEETLES (COLEOPTERA: COCCINELLIDAE) SO ENTOMOLOGICA AMERICANA LA English DT Article DE non-target effects; native aphids; Coccinella; Hippodamia convergens; Solidago; Cyclachaena xanthifolia AB Aphids in the genus Uroleucon Mordvilko (Hemiptera: Aphididae) are native herbivores that feed on goldenrod (Solidago spp.) and other Asteraceae in North America. The aphids are potential prey for a wide variety of natural enemies, including native and non-native species of lady beetles (Coleoptera: Coccinellidae). Non-native lady beetles were introduced to aid control of pest aphids in cropland, but some (e.g. Coccinella septempunctata L. and Harmonia axyridis [Pallas]) are known to prey upon Uroleucon aphids in natural and semi-natural settings, raising concerns about their nontarget effects on native aphids. The objective of this study was to estimate non-target risk to Uroleucon aphids from non-native lady beetles. Risk was estimated by evaluating consumption of Uroleucon aphids by lady beetles in no-choice laboratory tests, and by determining incidence of non-native lady beetles within naturally occurring patches of goldenrod and giant sumpweed (Cyclachaena xanthifolia (Nutt.) Fresen.) used by U. nigrotuberculatum (Olive) and U. ambrosiae (Thomas), respectively, in eastern South Dakota during 2009 and 2011. Although lady beetles substantially lowered populations of Uroleucon aphids in no-choice laboratory tests, they were infrequently associated with natural occurrences of the aphids, with incidence comprised of only four native Hippodamia convergens Guerin-Meneville and one non-native C. septempunctata on 185 aphid-infested sample units in relatively small patches of goldenrod and giant sumpweed. Despite consumption of aphids in laboratory tests, the particularly low incidence of lady beetles in field surveys indicates an empirically small risk of their predation on U. nigrotuberculatum and U. ambrosiae under field conditions. C1 USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Hesler, LS (reprint author), USDA ARS, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM louis.hesler@ars.usda.gov FU National Science Foundation [DRL-0741738]; USDA-ARS CRIS Project [5447-21220-005-00D] FX Eric Beckendorf, Phil Rozeboom, Sara Haberling, Sara Ackman and Ginger McNickle assisted with laboratory tests and field surveys. Colin Favret graciously identified aphids. Eric Beckendorf, Phil Rozeboom, Marissa Koerlin, Lauren Hesler, Paul J. Johnson, John Losey and Sharon Papiernik graciously reviewed drafts of this paper. This work was supported in part by a grant from the National Science Foundation, award number DRL-0741738, and by base funding under USDA-ARS CRIS Project No. 5447-21220-005-00D. NR 37 TC 0 Z9 0 U1 1 U2 6 PU NEW YORK ENTOMOLOGICAL SOC INC PI NEW YORK PA C/O AMER MUSEUM NAT HIST 79TH & CENTRAL PARK WEST, NEW YORK, NY 10024 USA SN 1947-5136 EI 1947-5144 J9 ENTOMOL AM-NY JI Entomol. Am. PD JUL PY 2013 VL 119 IS 1-2 BP 14 EP 22 DI 10.1664/13-RA-004R.1 PG 9 WC Entomology SC Entomology GA V35YA UT WOS:000209179800003 ER PT J AU Shappell, NW AF Shappell, Nancy W. TI Egg wash wastewater: Estrogenic risk or environmental asset? SO INTEGRATED ENVIRONMENTAL ASSESSMENT AND MANAGEMENT LA English DT Article AB Commercial production of eggs and egg products requires the washing of eggs to remove urinary-fecal material and broken egg residue. In the case of one Ohio farming facility, 1.6 million birds produce 1.4 million eggs per day, using approximately 50mL of wash water/egg or approximately 70000L per day. The aqueous waste stream was evaluated for estrogenicity to determine if potential for endocrine disruption would result from agricultural application of such wastewater. Samples collected the Fall (October) of 2010 included: water from 2 egg washers operating in series, inlet pipe to the treatment lagoon, a lagoon composite, and products used within the facility in the cleaning of equipment and treatment of the waste. In February 2011, the treatment lagoon was fitted with an extensive aeration system and subsequent sample sets were collected on 3 consecutive days in May and November. Samples were extracted by solid phase extraction and assayed for estrogenic activity using the in vitro E-Screen assay. Raw untreated wastewater from the egg washers contained 17-estradiol equivalents (E(2)Eqs) ranging from 9 to 18ng/L, pipe grab samples entering into the treatment lagoon ranged from <0.14 to 4.4ng/L (variability related to time of emptying of egg wash tanks), whereas treatment lagoon water contained 0.3 to 4.0ng/L E(2)Eq. Addition of an aeration system to the treatment lagoon eliminated surface frothing, reduced noxious odor emission, and E(2)Eqs were lower than the pre-aeration concentrations (4ng/L [n=1, no statistical comparison possible] vs 0.3 to 1.4ng/L in 2011). Because of matrix effects, estrogens were not quantifiable by LC-MS2 in even egg washwater extracts, at concentrations in which internal deuterated estrogen standards were quantifiable. Estrone and E-2 parent ions were detected in egg washwater samples only, and confirmatory ion fragments were detected in only one of these samples. Estrogenicity of the wastewater from the treatment lagoon was already at the proposed aquatic no effect concentration for 17-E2 and would be expected to decrease further as wastewater passes through 2 consecutive storage ponds before application on field crops for irrigation. The original project plan was to follow the wastewater as it was applied by aerial irrigation and concomitant surface runoff, but based on the consistent and extremely low concentration of estrogenic activity of the wastewater from the treatment lagoon, it was concluded that activity would be below limits of quantitation by E-Screen in water used for irrigation from the storage ponds. Use of egg wash wastewateror gray waterto irrigate crops removes the cost and burden of wastewater treatment by the local wastewater plant, poses little to no potential threat of estrogenic endocrine disruption, and supports the conservation of water resources through the use of wastewater irrigation. Integr Environ Assess Manag 2013;9:517-523. (c) 2013 SETAC RP Shappell, NW (reprint author), ARS, USDA, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND USA. EM nancy.shappell@ars.usda.gov NR 21 TC 0 Z9 0 U1 1 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1551-3777 EI 1551-3793 J9 INTEGR ENVIRON ASSES JI Integr. Environ. Assess. Manag. PD JUL PY 2013 VL 9 IS 3 BP 517 EP 523 DI 10.1002/ieam.1415 PG 7 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA 180RM UT WOS:000321612800028 PM 23441095 ER PT J AU Mercure, E Halasz, J Hegedus, A Ledbetter, C AF Mercure, Eric Halasz, Julia Hegedues, Attila Ledbetter, Craig TI Yield, pollination aspects and kernel qualities of almond (Prunus dulcis) selections trialed in the southern San Joaquin Valley SO JOURNAL OF THE AMERICAN POMOLOGICAL SOCIETY LA English DT Article DE alternate bearing; bloom phenology; double kernel; S-allele profile; variety trial AB A field trial was established in the southern San Joaquin Valley to determine yield potential for nine almond selections grown under commercial conditions. Kernel yields were first quantified in 2008 at the end of the third growing season and continued through the 2010 harvest. Harvested tonnage varied significantly, depending on the particular accession, specific field location and harvest year. The almond accessions were genotyped for S-locus alleles, characterized phenologically during bloom, and analyzed for kernel dimensions, pellicle color profiles, nut defects and harvest quality parameters. Bloom duration and overlap of the nine experimental accessions generally coincided with that of 'Nonpareil' during the three examined bloom periods. Analysis of S-alleles revealed semi- or cross-incompatible pairs with some commercial cultivars, and confirmed the self-compatibility status of four of the trialed selections. Harvest year effects were significant on all examined kernel shape and appearance characters as well as all nut defects and harvest quality parameters except for the level of blank and shriveled kernels. Cumulative kernel yield differences and specific character faults have led to the removal of five accessions from the trial after three harvest seasons. C1 [Mercure, Eric] Paramount Farming Co, Bakersfield, CA 93308 USA. [Halasz, Julia; Hegedues, Attila] Corvinus Univ Budapest, Dept Genet & Plant Breeding, H-1518 Budapest, Hungary. [Ledbetter, Craig] ARS, USDA, Crop Dis Pests & Genet Res Unit, Carlsbad, CA USA. RP Mercure, E (reprint author), Paramount Farming Co, 33141 E Lerdo Highway, Bakersfield, CA 93308 USA. FU ARS project, 'Improvement of Prunus and Vitis scions for fruit quality and pest resistance' [5302-21220-005-00D] FX This work was funded by the ARS project, 'Improvement of Prunus and Vitis scions for fruit quality and pest resistance' (5302-21220-005-00D). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 26 TC 0 Z9 0 U1 2 U2 4 PU AMER POMOLOGICAL SOC PI UNIVERSITY PK PA 102 TYSON BUILDING, UNIVERSITY PK, PA 16802 USA SN 1527-3741 J9 J AM POMOL SOC JI J. Amer. Pomolog. Soc. PD JUL PY 2013 VL 67 IS 3 BP 126 EP 136 PG 11 WC Agronomy; Horticulture SC Agriculture GA V36TE UT WOS:000209232700001 ER PT J AU Postman, J Kim, D Bassil, N AF Postman, Joseph Kim, Daeil Bassil, Nahla TI OH x F Paternity Perplexes Pear Producers SO JOURNAL OF THE AMERICAN POMOLOGICAL SOCIETY LA English DT Article AB Early in the 20th century, a collection of fire blight resistant pears from around the world was assembled in southern Oregon in an effort to develop improved rootstocks. 'Old Home' and 'Farmingdale' are two cultivars from Illinois that exhibited strong fire blight resistance and useful horticultural traits. Both became important as interstem stocks and as parents in the development of new rootstocks. In the 1950s, an Oregon nurseryman collected seed from an 'Old Home' tree in British Columbia purportedly pollinated by 'Farmingdale' and hundreds of numbered selections of this cross of 'Old Home' x 'Farmingdale' (OHxF) were evaluated. Several OHxF selections arc now valued as rootstocks worldwide, and 45 unique OH x F selections are maintained at the USDA-ARS National Clonal Germplasm Repository (NCGR), in Corvallis, Oregon. Simple Sequence Repeat (SSR) or microsatellite-based profiles were generated for 'Old Home', 'Farmingdale', 8 OHxF selections, and several reference pear cultivars at NCGR using a standard fingerprinting set developed by the European Cooperative Programme for Plant Genetic Resources. 'Farmingdale' is thought to be a seedling of 'Beurre d'Anjou'. Our study showed that 'Farmingdale' shared at least one SSR allele with 'Anjou' at each locus tested, confirming this parental relationship. Our studies showed that all OHxF selections shared an allele with 'Old Home' at each locus, with one allele carrying a suspected pair of base deletions. However, based on our SSR results, it is impossible for 'Farmingdale' to be the pollen parent for any oldie OHxF selections examined. Evaluation of the world pear collection at NCGR with this fingerprinting set established the cultivar 'Bartlett' as the actual pollen parent of these rootstock clones. Fruit and leaf morphology is also consistent with 'Bartlett' and not 'Farmingdale' as a parent of OHxF rootstock selections. The highly fire blight resistant 'Farmingdale' is apparently very under-represented in the pedigrees of current pear rootstocks, and deserves renewed consideration. C1 [Postman, Joseph; Bassil, Nahla] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97331 USA. [Kim, Daeil] Chungbuk Natl Univ, Dept Hort Sci, Cheongju 361763, Chungbuk, South Korea. RP Postman, J (reprint author), USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97331 USA. NR 27 TC 1 Z9 1 U1 1 U2 3 PU AMER POMOLOGICAL SOC PI UNIVERSITY PK PA 102 TYSON BUILDING, UNIVERSITY PK, PA 16802 USA SN 1527-3741 J9 J AM POMOL SOC JI J. Amer. Pomolog. Soc. PD JUL PY 2013 VL 67 IS 3 BP 157 EP 167 PG 11 WC Agronomy; Horticulture SC Agriculture GA V36TE UT WOS:000209232700004 ER PT J AU Cerdeira, AL Silva, FML Donega, MA Cantrell, CL Shea, K Duke, SO Velini, ED Corniani, N AF Cerdeira, A. L. Silva, F. M. L. Donega, M. A. Cantrell, C. L. Shea, K. Duke, S. O. Velini, E. D. Corniani, N. TI Roots of the Invasive Species Carduus nutans L. and C. acanthoides L. Produce the Phytotoxin Aplotaxene, a Possible Allelochemical SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Cerdeira, A. L.] Embrapa Environm, Div Res, Brazilian Dept Agr, BR-13820000 Jaguariuna, SP, Brazil. [Silva, F. M. L.; Velini, E. D.; Corniani, N.] Sao Paulo State Univ UNESP, BR-18610307 Botucatu, SP, Brazil. [Donega, M. A.] Univ Sao Paulo, BR-13418900 Piracicaba, SP, Brazil. [Cantrell, C. L.; Duke, S. O.] USDA ARS, Natl Prod Utilizat Res Unit, Oxford, MS 38677 USA. [Shea, K.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. RI Shea, Katriona/B-7954-2008 OI Shea, Katriona/0000-0002-7607-8248 NR 0 TC 0 Z9 0 U1 0 U2 3 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PB2 BP 826 EP 826 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100079 ER PT J AU Long, SA Dowd, PF Wicklow, DT Gloer, JB AF Long, S. A. Dowd, P. F. Wicklow, D. T. Gloer, J. B. TI A New Diketopiperazine from Trichothecium Crotocinigenum SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Long, S. A.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Dowd, P. F.; Wicklow, D. T.] ARS, Mycotoxin Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL3 BP 847 EP 848 PG 2 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100167 ER PT J AU Hwang, IH Gloer, JB Wicklow, DT AF Hwang, I. H. Gloer, J. B. Wicklow, D. T. TI Caryophyllene Sesquiterpenoids from a Fungicolous Isolate of Pestalotia sp. SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Hwang, I. H.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL11 BP 849 EP 849 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100175 ER PT J AU Phatak, NL Wicklow, DT Gloer, JB AF Phatak, N. L. Wicklow, D. T. Gloer, J. B. TI Herbarumin Analogues from an Endophytic Isolate of Phoma sp Isolated from a Seed of Bromus kalmia SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Phatak, N. L.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Mycotoxin Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL19 BP 851 EP 851 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100183 ER PT J AU Jayanetti, DR Wicklow, DT Gloer, JB AF Jayanetti, D. R. Wicklow, D. T. Gloer, J. B. TI New Hynapene Analogs from a Fungicolous Isolate of Penicillium chrysogenum SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Jayanetti, D. R.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL26 BP 852 EP 852 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100190 ER PT J AU Kaur, A Swenson, DC Wicklow, DT Gloer, JB AF Kaur, A. Swenson, D. C. Wicklow, D. T. Gloer, J. B. TI New Fiscalin, Tryptoquivaline, and Fumiquinazoline Analogues from an Endophytic Isolate of Neosartorya aureola SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Kaur, A.; Swenson, D. C.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL25 BP 852 EP 852 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100189 ER PT J AU Zuck, KM Rosario, M Widmer, TL Newman, DJ AF Zuck, K. M. Rosario, M. Widmer, T. L. Newman, D. J. TI New Analogs Produced by the Co-Culture of the Endophyte Xylaria sp and Phytophthora SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Zuck, K. M.] SAIC Frederick Inc, Frederick Natl Lab Canc Res, Nat Prod Support Grp, Frederick, MD 21702 USA. [Rosario, M.; Newman, D. J.] NCI, Frederick Natl Lab Canc Res, DTP, Nat Prod Branch, Frederick, MD 21702 USA. [Widmer, T. L.] ARS, USDA, FDWSRU, Ft Detrick, MD 21702 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL24 BP 852 EP 852 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100188 ER PT J AU Cannistra, JC Wicklow, DT Dowd, PF Gloer, JB AF Cannistra, J. C. Wicklow, D. T. Dowd, P. F. Gloer, J. B. TI New Peptides from a Fungicolous Isolate of Fusarium merismoides SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Cannistra, J. C.; Gloer, J. B.] Univ Iowa, Dept Chem, Iowa City, IA 52242 USA. [Wicklow, D. T.] ARS, Bacterial Foodbome Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Dowd, P. F.] ARS, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL28 BP 853 EP 853 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100192 ER PT J AU Rivera-Chavez, J Gonzalez-Andrade, M Gonzalez, MD Glenn, AE Mata, R AF Rivera-Chavez, J. Gonzalez-Andrade, M. del Carmen Gonzalez, M. Glenn, A. E. Mata, R. TI Thielavins A, J and K: alpha-Glucosidase Inhibitors from MEXU 27095, an Endophytic Fungus from Hintonia latiflora SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Rivera-Chavez, J.; Gonzalez-Andrade, M.; Mata, R.] Univ Nacl Autonoma Mexico, Fac Quim, Mexico City 04510, DF, Mexico. [del Carmen Gonzalez, M.] Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico. [Glenn, A. E.] ARS, Toxicol & Mycotoxin Res Unit, USDA, Russell Res Ctr, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PL35 BP 854 EP 854 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100199 ER PT J AU Oh, J Bowling, JJ Zou, Y Chittiboyina, AG Doerksen, RJ Ferreira, D Leininger, TD Hamann, MT AF Oh, J. Bowling, J. J. Zou, Y. Chittiboyina, A. G. Doerksen, R. J. Ferreira, D. Leininger, T. D. Hamann, M. T. TI Configurational Assignments of Conformationally Restricted Bis-Monoterpene Hydroquinones: Utility in Exploration of Endangered Plants SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Oh, J.; Bowling, J. J.; Zou, Y.; Ferreira, D.; Hamann, M. T.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA. [Oh, J.; Bowling, J. J.; Zou, Y.; Doerksen, R. J.; Ferreira, D.; Hamann, M. T.] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, University, MS 38677 USA. [Chittiboyina, A. G.] Univ Mississippi, Natl Ctr Nat Prod Res, Thad Cochran Res Ctr, University, MS 38677 USA. [Doerksen, R. J.] Univ Mississippi, Dept Med Chem, Sch Pharm, University, MS 38677 USA. [Leininger, T. D.] US Forest Serv, Ctr Bottomland Hardwoods Res, USDA, Stoneville, MS 38776 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PN52 BP 868 EP 868 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100256 ER PT J AU Sims, JW Christensen, S Huffaker, A Teal, P Schmelz, E AF Sims, J. W. Christensen, S. Huffaker, A. Teal, P. Schmelz, E. TI Isolation and Identification of Novel Terpenoid Phytoalexins in Maize SO PLANTA MEDICA LA English DT Meeting Abstract CT Annual Meeting of the American-Society-of-Pharmacognosy on Natural Products at a Crossroad - Current and Future Directions CY JUL 14-17, 2013 CL St Louis, MO SP Amer Soc Pharmacognosy C1 [Sims, J. W.; Christensen, S.; Huffaker, A.; Teal, P.; Schmelz, E.] ARS, Chem Unit, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL 32608 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD JUL PY 2013 VL 79 IS 10 MA PN59 BP 870 EP 870 PG 1 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AK8RG UT WOS:000338695100263 ER PT J AU Bailey, SW AF Bailey, Scott W. TI TWO FERN SPECIES NEW TO NEW HAMPSHIRE, WITH COMMENTS ON THE GENERATION OF CALCAREOUS-LIKE HABITAT BY BASE-POOR ROCKS SO RHODORA LA English DT Article C1 US Forest Serv, No Res Stn, North Woodstock, NH 03262 USA. RP Bailey, SW (reprint author), US Forest Serv, No Res Stn, 234 Mirror Lake Rd, North Woodstock, NH 03262 USA. EM swbailey@fs.fed.us OI Bailey, Scott/0000-0002-9160-156X NR 6 TC 2 Z9 2 U1 0 U2 2 PU NEW ENGLAND BOTANICAL CLUB INC PI LAWRENCE PA P.O. BOX 368, LAWRENCE, KS 66044 USA SN 0035-4902 EI 1938-3401 J9 RHODORA JI Rhodora PD JUL PY 2013 VL 115 IS 963 BP 286 EP 289 DI 10.3119/13-10 PG 4 WC Plant Sciences SC Plant Sciences GA AI7TM UT WOS:000337101700004 ER PT J AU Richardson, KA West, SD Gitzen, RA AF Richardson, Kristen A. West, Stephen D. Gitzen, Robert A. TI CHEATGRASS (BROMUS TECTORUM) DOMINATES CHEEK POUCH CONTENTS OF THE GREAT BASIN POCKET MOUSE (PEROGNATHUS PARVUS) SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID SMALL MAMMALS; SHRUB-STEPPE; EASTERN WASHINGTON; BODY CONDITION; COMMUNITIES; SHRUBSTEPPE; HABITAT; DESERT; ABUNDANCE; WILDFIRE AB Most of the native shrubsteppe habitat in the northern Columbia Basin of eastern Washington has been invaded by cheatgrass (Bromus tectorum) or converted to agricultural lands. Therefore, ecological patterns and dynamics on native shrubsteppe and reestablished grasslands are of high conservation interest. We analyzed the cheek pouch contents of Great Basin pocket mice (Perognathus parvus) from 48 study sites in this region to quantify seed collection by this species and to determine the influence of habitat type on cheek pouch seed contents. In all 3 habitat types-newly established Conservation Reserve Program (CRP) lands, older CRP lands, and shrubsteppe-B. tectorum constituted the majority of seeds collected from the cheek pouches. Mean generic richness of collected seeds was higher in shrubsteppe than in new CRP habitats. On average, females collected a greater number of seed genera than males did, and pocket mice collected more seed genera as the autumn season progressed. Exotic B. tectorum has become the most frequently collected autumn food resource for pocket mice in the northern Columbia Basin. C1 [Richardson, Kristen A.; West, Stephen D.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Gitzen, Robert A.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. RP Richardson, KA (reprint author), US Forest Serv, Okanogan Wenatchee Natl Forest, 215 Melody Lane, Wenatchee, WA 98801 USA. EM kar4@uw.edu FU WDFW through USFWS grants FX This study was funded in part by M. Vander Haegen and the WDFW through USFWS grants for the small mammal trapping; M. Vander Haegen and M. Schroeder designed the underlying comparison of habitat types and landscape contexts, and provided vegetation data. Sampling of mice and extraction of contents from cheek pouches was coordinated by M. Kroeger, with assistance from E. Murphy, J. Stebbins, L. Malone, and S. Anderson. We thank L. Conquest and J. Skalski for guidance with statistical analyses; D. Giblin for access to collections at the University of Washington Herbarium; S. Doty for use of equipment; K. Stuart for editing; K. Williams-Guillen and A. Johnston for providing valuable comments throughout the study; and L. Stacey for insect identification. NR 40 TC 1 Z9 1 U1 1 U2 4 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD JUL PY 2013 VL 73 IS 2 BP 158 EP 167 DI 10.3398/064.073.0205 PG 10 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM6GB UT WOS:000339960300004 ER PT J AU McKelvey, KS Ramirez, JE Pilgrim, KL Cushman, SA Schwartz, MK AF McKelvey, Kevin S. Ramirez, Jennifer E. Pilgrim, Kristine L. Cushman, Samuel A. Schwartz, Michael K. TI GENETIC SAMPLING OF PALMER'S CHIPMUNKS IN THE SPRING MOUNTAINS, NEVADA SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID EFFECTIVE POPULATION-SIZE; HYBRIDIZATION; SEQUENCES AB Palmer's chipmunk (Neotamias palmeri) is a medium-sized chipmunk whose range is limited to the higher-elevation areas of the Spring Mountain Range, Nevada. A second chipmunk species, the Panamint chipmunk (Neotamias panamintinus), is more broadly distributed and lives in lower-elevation, primarily pinyon-juniper (Pinus monophylla-Juniperus osteosperma) habitat types. Panamint chipmunks are not closely related to Palmer's, but field identification of the 2 species is unreliable. Palmer's chipmunk is a species of concern in the state of Nevada and is listed by the International Union for Conservation of Nature (IUCN) as endangered. As such, conservation of Palmer's chipmunks is a priority in the Spring Mountains National Recreation Area. We sampled putative Palmer's chipmunks from 13 sites distributed across the Spring Mountains during 2010-2011. We removed Panamint chipmunks by using DNA-based identifications and then analyzed the genetic population structure of Palmer's chipmunks by using a panel of 9 microsatellites. Of the 228 samples that were genotyped, 186 were Palmer's; there was no evidence of hybridization between species. Four sites had exclusively Panamint chipmunks, 5 had exclusively Palmer's chipmunks, and 3 had a mixture of the 2 species. In this study, Palmer's chipmunks were exclusively captured at sites above 2400 m elevation, and Panamint chipmunks were exclusively captured at sites below 2200 m. Panamint chipmunks were trapped in areas typed as pinyon-juniper, but they were also trapped at sites typed as ponderosa pine (Pinus ponderosa) and mixed conifer. Both species were trapped at 3 sites; at all 3 sites, the lower-elevation traps contained Panamint chipmunks and the higher ones Palmer's chipmunks. Population structure within Palmer's chipmunks was minimal: heterozygosity was relatively high, and the populations displayed no signs of recent bottlenecks. Indications are that the distribution of Palmer's chipmunk is limited to higher-elevation areas in the Spring Mountains, but within this area, Palmer's chipmunk occurs as a single, large, well-connected, and stable population. C1 [McKelvey, Kevin S.; Pilgrim, Kristine L.; Schwartz, Michael K.] USDA, Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Ramirez, Jennifer E.] USDA, Forest Serv, Spring Mt Natl Recreat Area, Las Vegas, NV 89130 USA. [Cushman, Samuel A.] USDA, Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP McKelvey, KS (reprint author), USDA, Forest Serv, Rocky Mt Res Stn, 800 East Beckwith, Missoula, MT 59801 USA. EM kmckelvey@fs.fed.us RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU Southern Nevada Public Lands Management Act FX This study was funded by the Southern Nevada Public Lands Management Act. We wish to thank the Great Basin Institute for providing field crews and Cory Engkjer for laboratory work on the DNA analyses. Additionally, we thank Daniel Bingham and Christopher Lowrey for their comments on earlier drafts. Lastly, we wish to thank 2 anonymous reviewers. NR 27 TC 0 Z9 0 U1 1 U2 4 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD JUL PY 2013 VL 73 IS 2 BP 198 EP 210 DI 10.3398/064.073.0209 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM6GB UT WOS:000339960300008 ER PT J AU Wang, R Kumar, M Marks, D AF Wang, Rui Kumar, Mukesh Marks, Danny TI Anomalous trend in soil evaporation in a semi-arid, snow-dominated watershed SO ADVANCES IN WATER RESOURCES LA English DT Article DE PIHM; ISNOBAL; Coupling; Snow covered area (SCA); Modeling ID RIVER-BASIN; CLIMATE; CATCHMENT; STREAMFLOW; BALANCE; FLOW; AVAILABILITY; GENERATION; SIMULATION; PATTERNS AB Soil evaporation in arid and semi-arid regions is generally moisture-limited. Evaporation in these regions is expected to increase monotonically with increase in precipitation. In contrast, model simulations in a snow-dominated, semi-arid Reynolds Mountain East (RME) watershed point to the existence of an anomalous trend in soil evaporation. Results indicate that soil evaporation in snow-dominated watersheds first increases and then subsequently decreases with increasing precipitation. The anomalous variation is because of two competing evaporation controls: (a) higher soil moisture in wetter years which leads to larger evaporation, and (b) prolonged snow cover period in wetter years which shields the soil from the atmosphere, thus reducing soil evaporation. To further evaluate how the competition is mediated by meteorological and hydrogeological characteristics of the watershed, changes in the trend due to different watershed hydraulic conductivity, vegetation cover, and snowfall area fraction are systematically studied. Results show considerable persistence in the anomalous trend over a wide range of controls. The controlling factors, however, have significant influence both on the magnitude of the WY evaporation and the location of the inflection point in the trend curve. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Wang, Rui; Kumar, Mukesh] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Marks, Danny] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. RP Kumar, M (reprint author), Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. EM mukesh.kumar@duke.edu FU Duke University; USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West [5362-13610-008-00D] FX This study was supported by the Duke University start-up grant for new faculties, and the data and analysis presented in this paper were funded in part by USDA-ARS CRIS Snow and Hydrologic Processes in the Intermountain West (5362-13610-008-00D). Duke University and USDA are equal opportunity providers and employers. We thank Jeff Dozier and two other anonymous reviewers for constructive comments that greatly improved this paper. NR 31 TC 11 Z9 11 U1 0 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0309-1708 EI 1872-9657 J9 ADV WATER RESOUR JI Adv. Water Resour. PD JUL PY 2013 VL 57 BP 32 EP 40 DI 10.1016/j.advwatres.2013.03.004 PG 9 WC Water Resources SC Water Resources GA 149PG UT WOS:000319332500004 ER PT J AU Hay-Roe, MM Meagher, RL Nagoshi, RN AF Hay-Roe, Mirian M. Meagher, Robert L. Nagoshi, Rodney N. TI Effect of fall armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae) strain and diet on oviposition and development of the parasitoid Euplectrus platyhypenae (Hymenoptera: Eulophidae) SO BIOLOGICAL CONTROL LA English DT Article DE Allelochemical; Biological control; Ectoparasitoid; Fall armyworm; Spodoptera frugiperda; Euplectrus platyhypenae ID INDUCED PLANT VOLATILES; HOST STRAINS; CYANOGENIC GLUCOSIDES; HELICONIINI INSECTA; I GENE; IDENTIFICATION; BIOSYNTHESIS; LOTAUSTRALIN; PLATHYPENAE; COMSTOCKII AB Oviposition tendency and subsequent development of the parasitoid wasp Euplectrus platyhypenae Howard was compared using the corn and rice host strains of Spodoptera frugiperda (J. E. Smith) fed corn (Zea mays, 'Truckers Favorite') or stargrass (Cynodon nlemfuensis Vanderyst var. nlemfuensis, 'Florona'). In no-choice and two-choice experiments using newly molted fourth instar hosts, female wasps preferred to oviposit on corn strain larvae fed corn plants. Rice strain larvae were parasitized when fed corn plants, but only after the host had reached an optimal size. This preference might be due to differences in size between host strains at a given stage. No oviposition preference for corn or rice strain larvae fed grass was observed. When female wasps were given a choice between corn and rice strain larvae, they always produced more female offspring on the corn strain than the rice strain larvae, irrespective of the host plant the larvae fed upon. Among rice strain hosts, those fed corn hosted more female offspring than those fed grass. The data also show that host quality had a direct effect on parasitoid size, since larger parasitoid offspring resulted from the largest host fed corn plants, and smaller offspring resulted from the smallest hosts, rice strain larvae fed stargrass. Furthermore, E. platyhypenae larval mortality occurred in hosts of both strains fed stargrass, which may indicate that stargrass allelochemicals are not appropriate for this parasitoid. Implications of these results for biological control methods are discussed. C1 [Hay-Roe, Mirian M.; Meagher, Robert L.; Nagoshi, Rodney N.] ARS, Behav & Biocontrol Unit, USDA, CMAVE, Gainesville, FL 32608 USA. RP Hay-Roe, MM (reprint author), ARS, Behav & Biocontrol Unit, USDA, CMAVE, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM mmhr@ufl.edu RI sebastianovitsch, stepan/G-8507-2013 NR 38 TC 1 Z9 1 U1 0 U2 30 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2013 VL 66 IS 1 BP 21 EP 26 DI 10.1016/j.biocontrol.2013.03.003 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 145CX UT WOS:000318992700004 ER PT J AU Shapiro-Ilan, DI Brown, I AF Shapiro-Ilan, David I. Brown, Ian TI Earthworms as phoretic hosts for Steinernema carpocapsae and Beauveria bassiana: Implications for enhanced biological control SO BIOLOGICAL CONTROL LA English DT Article DE Beauveria; Earthworm; Entomopathogenic; Pecan weevil; Nematode; Phoresy; Steinernema ID CURCULIO-CARYAE COLEOPTERA; ENTOMOPATHOGENIC NEMATODES; METARHIZIUM-ANISOPLIAE; HYLOBIUS-ABIETIS; SWEET-PEPPER; DISPERSAL; SOIL; SYSTEMS; FUNGI; RHABDITIDA AB Prior research indicated that earthworms may serve as phoretic hosts to entomopathogenic nematodes. Therefore, we hypothesized that biocontrol efficacy of entomopathogenic nematodes could be enhanced in the presence of earthworms based on increased nematode dispersal through the soil. We also hypothesized that earthworms may serve as phoretic hosts to other entomopathogens, such as entomopathogenic fungi. We tested the first hypothesis by comparing the ability of two strains of Steinernema carpocapsae to suppress the pecan weevil, Curculio caryae, in the presence or absence of the earthworm Lumbricus terrestris. Under greenhouse conditions, S. carpocapsae was applied to the soil surface in pots containing C. caryae larvae. We tested the second hypothesis by determining the effects of L. terrestris on dispersal of the entomopathogenic fungus Beauveria bassiana in soil columns. Our results indicated that earthworm presence improved the ability of S. carpocapsae (Sal strain) to control C. caryae below the soil surface. Additionally, S. carpocapsae (All strain) only caused significant C. caryae suppression relative to the controls in the presence of earthworms. B. bassiana dispersal was enhanced by earthworm presence. The fungus was carried through soil by the earthworm; conidia recovered from the earthworm casts remained viable and was pathogenic to Galleria mellonella. Earthworms and other phoretic hosts may assist in dispersal of entomopathogenic nematode and fungal populations in various ecosystems and thereby assist in regulation of insect pests. Additionally, it may be conceivable to combine the application of earthworms with entomopathogens to achieve enhanced levels of inundative or inoculative biocontrol. C1 [Shapiro-Ilan, David I.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Brown, Ian] Georgia Southwestern Univ, Dept Biol, Americus, GA USA. RP Shapiro-Ilan, DI (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM David.Shapiro@ars.usda.gov NR 42 TC 3 Z9 3 U1 5 U2 48 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD JUL PY 2013 VL 66 IS 1 BP 41 EP 48 DI 10.1016/j.biocontrol.2013.03.005 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA 145CX UT WOS:000318992700007 ER PT J AU Arismendi, I Safeeq, M Johnson, SL Dunham, JB Haggerty, R AF Arismendi, Ivan Safeeq, Mohammad Johnson, Sherri L. Dunham, Jason B. Haggerty, Roy TI Increasing synchrony of high temperature and low flow in western North American streams: double trouble for coldwater biota? SO HYDROBIOLOGIA LA English DT Article DE Climate change; Freshwater ecosystems; Hydrology; Temperature; Hydroclimatology ID UNITED-STATES; OREGON; VARIABILITY; POPULATIONS; MORPHOLOGY; HYDROLOGY; DYNAMICS; DROUGHT; REGIMES; TRENDS AB Flow and temperature are strongly linked environmental factors driving ecosystem processes in streams. Stream temperature maxima (T (max_w)) and stream flow minima (Q (min)) can create periods of stress for aquatic organisms. In mountainous areas, such as western North America, recent shifts toward an earlier spring peak flow and decreases in low flow during summer/fall have been reported. We hypothesized that an earlier peak flow could be shifting the timing of low flow and leading to a decrease in the interval between T (max_w) and Q (min). We also examined if years with extreme low Q (min) were associated with years of extreme high T (max_w). We tested these hypotheses using long-term data from 22 minimally human-influenced streams for the period 1950-2010. We found trends toward a shorter time lag between T (max_w) and Q (min) over time and a strong negative association between their magnitudes. Our findings show that aquatic biota may be increasingly experiencing narrower time windows to recover or adapt between these extreme events of low flow and high temperature. This study highlights the importance of evaluating multiple environmental drivers to better gage the effects of the recent climate variability in freshwaters. C1 [Arismendi, Ivan; Safeeq, Mohammad; Haggerty, Roy] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Johnson, Sherri L.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. RP Arismendi, I (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. EM ivan.arismendi@oregonstate.edu RI Haggerty, Roy/A-5863-2009; Arismendi, Ivan/B-3144-2010 OI Arismendi, Ivan/0000-0002-8774-9350 FU US Geological Survey; US Forest Service Pacific Northwest Research Station; Oregon State University FX Brooke Penaluna, Tim D. Mayer, two anonymous referees, and the associated editor provided comments on the manuscript. Financial support was provided by US Geological Survey, the US Forest Service Pacific Northwest Research Station and Oregon State University. Use of firm or trade names is for reader information only and does not imply endorsement of any product or service by the U.S. Government. NR 42 TC 21 Z9 21 U1 4 U2 62 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0018-8158 J9 HYDROBIOLOGIA JI Hydrobiologia PD JUL PY 2013 VL 712 IS 1 BP 61 EP 70 DI 10.1007/s10750-012-1327-2 PG 10 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA 142IM UT WOS:000318790800005 ER PT J AU Luo, YC Wang, TTY Teng, Z Chen, P Sun, JH Wang, Q AF Luo, Yangchao Wang, Thomas T. Y. Teng, Zi Chen, Pei Sun, Jianghao Wang, Qin TI Encapsulation of indole-3-carbinol and 3,3 '-diindolylmethane in zein/carboxymethyl chitosan nanoparticles with controlled release property and improved stability SO FOOD CHEMISTRY LA English DT Article DE Indole-3-carbinol; Diindolylmethane; Zein; Carboxymethyl chitosan; Encapsulation; Stability ID ACID CONDENSATION PRODUCTS; DELIVERY APPLICATIONS; CANCER CELLS; TWEEN 20; ZEIN; MICE; DIINDOLYLMETHANE; DERIVATIVES; PROTEINS; TISSUE AB Indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM) are two bioactive compounds from Cruciferous vegetables. The stability of these compounds is a major challenge for their pharmaceutical applications. In this study, zein and zein/carboxymethyl chitosan (CMCS) nanoparticles were prepared to encapsulate I3C and DIM by a combined liquid-liquid phase separation and ionic gelation method. After zein nanoparticles were coated with CMCS, the zeta potential was decreased from around -10 to -20 mV, and encapsulation efficiency was greatly improved. Both nanoparticle formulations provided controlled release of I3C and DIM in PBS medium. Zein and zein/CMCS nanoparticles demonstrated similar protection for both I3C and DIM against ultraviolet (UV) light, attributed mainly to the contribution of the zein protein. Compared with zein nanoparticles, zein/CMCS nanoparticles exhibited better protection of I3C against degradation and better inhibition against its oligomerization to DIM under thermal condition (37 degrees C). Based on our results, the encapsulation of hydrophobic bioactives in zein/CMCS nanoparticles is a promising approach to improve their stability against harsh conditions and provide controlled release for food/pharmaceutical applications. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Luo, Yangchao; Teng, Zi; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Wang, Thomas T. Y.] USDA, Human Nutr Res Ctr, ARS, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Chen, Pei; Sun, Jianghao] USDA, Human Nutr Res Ctr, ARS, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. RP Wang, Q (reprint author), Univ Maryland, Dept Nutr & Food Sci, 0112 Skinner Bldg,Coll Pk, College Pk, MD 20742 USA. EM wangqin@umd.edu RI Luo, Yangchao/I-2689-2012; Teng, Zi/G-5747-2013 OI Luo, Yangchao/0000-0002-0001-1194; Teng, Zi/0000-0002-6029-7024 FU Maryland Nanocenter at the University of Maryland FX We appreciate the support from Maryland Nanocenter at the University of Maryland. Authors are also thankful to Ms Xiangan Wang for her help with HPLC sample extraction. NR 40 TC 48 Z9 49 U1 12 U2 158 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUL 1 PY 2013 VL 139 IS 1-4 BP 224 EP 230 DI 10.1016/j.foodchem.2013.01.113 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 134HL UT WOS:000318200900031 PM 23561099 ER PT J AU Chu, YF Wise, ML Gulvady, AA Chang, T Kendra, DF van Klinken, BJW Shi, YH O'Shea, M AF Chu, Yi-Fang Wise, Mitchell L. Gulvady, Apeksha A. Chang, Tony Kendra, David F. van Klinken, B. Jan-Willem Shi, Yuhui O'Shea, Marianne TI In vitro antioxidant capacity and anti-inflammatory activity of seven common oats SO FOOD CHEMISTRY LA English DT Article DE Oats; Avenanthramides; ORAC; NF-kappa B; Antioxidant; Anti-inflammatory ID NF-KAPPA-B; AVENA-SATIVA L.; OXIDATIVE STRESS; HEALTH-BENEFITS; IMMUNE-SYSTEM; AVENANTHRAMIDES; INFLAMMATION; ACTIVATION; CANCER; ASSAY AB Oats are gaining increasing scientific and public interest for their purported antioxidant-associated health benefits. Most reported studies focused on specific oat extracts or particular oat components, such as beta-glucans, tocols (vitamin E), or avenanthramides. Studies on whole oats with respect to antioxidant and anti-inflammatory activities are still lacking. Here the antioxidant and anti-inflammatory activities from whole oat groats of seven common varieties were evaluated. All oat varieties had very similar oxygen radical absorption capacity compared with other whole grains. In an anti-inflammatory assay, oat variety CDC Dancer inhibited tumor necrosis factor-alpha induced nuclear factor-kappa B activation by 27.5% at 2 mg/ml, whereas variety Deiter showed 13.7% inhibition at a comparable dose. Avenanthramide levels did not correlate with the observed antioxidant and anti-inflammatory activities. Further investigations are needed to pinpoint the specific antioxidant and anti-inflammatory compounds, and potential synergistic and/or matrix effects that may help explain the mechanisms of oat's anti-inflammatory actions. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Chu, Yi-Fang; Gulvady, Apeksha A.; van Klinken, B. Jan-Willem; Shi, Yuhui; O'Shea, Marianne] PepsiCo R&D Nutr, Barrington, IL 60010 USA. [Wise, Mitchell L.] ARS Cereal Crops Res, USDA, Madison, WI 53726 USA. [Chang, Tony] Int Chem Testing, Milford, MA 01757 USA. [Kendra, David F.] PepsiCo R&D Agron, Barrington, IL 60010 USA. RP Chu, YF (reprint author), PepsiCo R&D Nutr, 617 W Main St, Barrington, IL 60010 USA. EM yifang.chu@pepsico.com NR 39 TC 26 Z9 28 U1 0 U2 76 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUL 1 PY 2013 VL 139 IS 1-4 BP 426 EP 431 DI 10.1016/j.foodchem.2013.01.104 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 134HL UT WOS:000318200900059 PM 23561127 ER PT J AU Shao, DY Bartley, GE Yokoyama, W Pan, ZL Zhang, HJ Zhang, A AF Shao, Dongyan Bartley, Glenn E. Yokoyama, Wallace Pan, Zhongli Zhang, Huijuan Zhang, Ang TI Plasma and hepatic cholesterol-lowering effects of tomato pomace, tomato seed oil and defatted tomato seed in hamsters fed with high-fat diets SO FOOD CHEMISTRY LA English DT Article DE Tomato pomace byproducts; Bile acid; Cholesterol; Hamster; CYP7A1; CYP51 ID GENE-EXPRESSION; LDL CHOLESTEROL; METABOLISM; LIVER; RATS; SOY AB The cholesterol-lowering effects of tomato pomace (TP), tomato seed oil (TS0) and defatted tomato seed (DTS) were determined in male Golden Syrian hamsters. Hamsters fed high-fat diets containing 10% TSO or 18% DTS were compared to a diet containing 10% corn oil and 10% microcrystalline cellulose (control 1), 42% TP were compared to 25% microcrystalline cellulose (control 2). TP, TSO and DTS reduced hepatic total cholesterol (TC) content. DTS also lowered plasma TC and low-density lipoprotein cholesterol (LDLC) concentrations. Fecal excretion of lipid, bile acid and cholesterol increased in the DTS group compared to control 1. DTS-fed hamsters had higher levels of hepatic CYP7A1, CYP51, ABCB11, and ABCG5 gene expression than control, suggesting both hepatic bile acid and cholesterol synthesis increased due to increased fecal excretion of bile acid and cholesterol. The results suggest that protein, dietary fibre or phenolic compounds in DTS may be responsible for plasma cholesterol decrease. Published by Elsevier Ltd. C1 [Shao, Dongyan] Northwest Agr & Forestry Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Shao, Dongyan; Pan, Zhongli; Zhang, Ang] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Bartley, Glenn E.; Yokoyama, Wallace; Pan, Zhongli; Zhang, Huijuan] ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Zhang, Huijuan] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China. [Zhang, Ang] Northwest Agr & Forestry Univ, Coll Enol, Yangling 712100, Shaanxi, Peoples R China. RP Yokoyama, W (reprint author), ARS, Proc Foods Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM Wally.Yokoyama@ARS.USDA.GOV; zlpan@ucdavis.edu NR 30 TC 10 Z9 12 U1 0 U2 67 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUL 1 PY 2013 VL 139 IS 1-4 BP 589 EP 596 DI 10.1016/j.foodchem.2013.01.043 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 134HL UT WOS:000318200900081 PM 23561149 ER PT J AU Lee, J Skinkis, PA AF Lee, Jungmin Skinkis, Patricia A. TI Oregon 'Pinot noir' grape anthocyanin enhancement by early leaf removal SO FOOD CHEMISTRY LA English DT Article DE Leaf pull; Defoliation; Pigment; Colour; Quality; Canopy management ID VITIS-VINIFERA L.; CABERNET FRANC; WINE COMPOSITION; CARBOHYDRATE RESERVES; NATURAL COLORANTS; CANOPY MANAGEMENT; EARLY DEFOLIATION; FRUIT COMPOSITION; ENZYME IMPACTS; BERRY GROWTH AB Complete cluster zone leaf removal of 'Pinot noir' was initiated at three separate pre-veraison growth stages (bloom, grain-pea size, and bunch closure) and maintained leaf free until harvest, for four growing seasons (2008-2011). Fruit anthocyanin composition was examined at harvest for the last two vintages (2010 and 2011) and compared to a control-no cluster zone leaf removal. Experiments were conducted at two commercially operating Oregon vineyards (site A = 420 rootstock/'Pinot noir' 115 scion and site B = 3309C rootstock/'Pinot noir' 777 scion). All clusters contained the five anthocyanins typically found in 'Pinot noir'. Leaf removal at bloom and maintained until harvest produced maximum anthocyanin accumulation in 'Pinot noir' grapes (site A = 85.24 mg/100 g and site B = 125.06 mg/100 g), compared to no leaf removal (control: site A = 57.91 mg/100 g and site B = 97.56 mg/100 g). Even leaf removal at bunch closure (last leaf removal initiation period) increased grape anthocyanin (site A 73.22 mg/bog and site B = 118.93 mg/100 g) compared to control, but total anthocyanins were lower than grapes from bloom leaf removal (first time period). Results differed slightly by vineyard site and rootstock/scion combination. Published by Elsevier Ltd. C1 [Lee, Jungmin] ARS, USDA, Hort Crops Res Unit Worksite, Parma, ID 83660 USA. [Skinkis, Patricia A.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. RP Lee, J (reprint author), ARS, USDA, PWA, HCRU Worksite, 29603 U 1 Ln, Parma, ID 83660 USA. EM jungmin.lee@ars.usda.gov; skinki-sp@hort.oregonstate.edu RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 NR 49 TC 17 Z9 17 U1 4 U2 60 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JUL 1 PY 2013 VL 139 IS 1-4 BP 893 EP 901 DI 10.1016/j.foodchem.2013.02.022 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 134HL UT WOS:000318200900119 PM 23561187 ER PT J AU Marklund, M McKeown, NM Blumberg, JB Chen, CYO AF Marklund, Matti McKeown, Nicola M. Blumberg, Jeffrey B. Chen, C. -Y. Oliver TI Hepatic biotransformation of alkylresorcinols is mediated via cytochrome P450 and beta-oxidation: A proof of concept study SO FOOD CHEMISTRY LA English DT Article DE Alkyresorcinols; Cytochrome P450; Liver; beta-Oxidation; Whole grains ID CANCER CELL-GROWTH; WHOLE-GRAIN WHEAT; CHROMATOGRAPHY-MASS SPECTROMETRY; HUMAN PLASMA; SWEDISH ADULTS; IN-VITRO; RYE; BRAN; METABOLITES; BIOMARKERS AB Alkylresorcinols (AR) are phenolic lipids present in the bran of some cereals. AR may serve as a biomarker for whole grain wheat and rye intake. While AR pharmacokinetics and two major metabolites have been reported, the metabolic pathways contributing to their relatively rapid elimination from the circulation remain to be speculative. In this study, we investigated if omega- and beta-oxidation mediate catabolism of the AR homologue C19:0 to form 3,5-dihydroxybenzoic acid and 3-(3,5-dihydroxyphenyl)-1-propanoic acid (DHPPA), using 3 in vitro platforms, human cytochrome P450 4F2 (CYP4F2), human liver S9, and HepG2 cells. One hydroxylated C19:0 metabolite was formed by CYP4F2 and one hydroxylated and one carboxylated C19:0 were tentatively identified after incubation of AR with 59. The formation of DHPPA was quantifiable when HepG2 cells were treated with C19:0 for 48 h. Our results are consistent with a metabolic pathway by which AR are degraded to phenolic acids via CYP4F2-mediated omega-oxidation and subsequent beta-oxidation. 2013 Elsevier Ltd. All rights reserved. C1 [Marklund, Matti; McKeown, Nicola M.; Blumberg, Jeffrey B.; Chen, C. -Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Chen, CYO (reprint author), Tufts Univ, Antioxidants Res Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM oliver.chen@tufts.edu OI Marklund, Matti/0000-0002-3320-796X FU General Mills Inc.; U.S. Department of Agriculture (USDA)/Agricultural Research Service [58-1950-7-707] FX This work was supported by the General Mills Inc. and the U.S. Department of Agriculture (USDA)/Agricultural Research Service (Cooperative Agreement No. 58-1950-7-707). The contents of this publication do not necessarily reflect the views or policies of the USDA nor does mention of trade names, commercial products or organizations imply endorsement by the U.S. government. All authors have no conflicts of interest. NR 25 TC 8 Z9 8 U1 0 U2 40 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 J9 FOOD CHEM JI Food Chem. PD JUL 1 PY 2013 VL 139 IS 1-4 BP 925 EP 930 DI 10.1016/j.foodchem.2013.01.122 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 134HL UT WOS:000318200900123 PM 23561191 ER PT J AU Kim, S Evans, K Biswas, A AF Kim, S. Evans, K. Biswas, A. TI Production of BSA-poly(ethyl cyanoacrylate) nanoparticles as a coating material that improves wetting property SO COLLOIDS AND SURFACES B-BIOINTERFACES LA English DT Article DE Nanoparticles; Cyanoacrylate; Wetting; Hydrophilic coating; Adsorption; Protein; Star copolymer ID QUARTZ-CRYSTAL MICROBALANCE; POLYMERIZATION AB Alkyl cyanoacrylates have long been used for the synthesis of colloidal nanoparticles. In the involved polymerization reaction, hydroxyl ions derived from dissociation of water have been used as an initiator. In the current research, an animal protein, bovine serum albumin (BSA) molecules were utilized as initiator for the polymerization. Following this reaction scheme, hydrophobic poly(ethyl cyanoacrylate)s were covalently bound to BSA, which is hydrophilic. Therefore, the resultant copolymer was amphiphilic in nature, and formed nanoparticles in the reaction medium. The suspension containing these nanoparticles showed an excellent coating capability on the surface of hydrophobic materials. A simple spray coating changed the wetting property of the material instantly and dramatically. Published by Elsevier B.V. C1 [Kim, S.; Evans, K.; Biswas, A.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Kim, S (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM sanghoon.kim@ars.usda.gov NR 21 TC 3 Z9 3 U1 3 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0927-7765 J9 COLLOID SURFACE B JI Colloid Surf. B-Biointerfaces PD JUL 1 PY 2013 VL 107 BP 68 EP 75 DI 10.1016/j.colsurfb.2013.01.064 PG 8 WC Biophysics; Chemistry, Physical; Materials Science, Biomaterials SC Biophysics; Chemistry; Materials Science GA 133MG UT WOS:000318141900009 PM 23466544 ER PT J AU Wiederoder, MS Liu, N Lefcourt, AM Kim, MS Lo, YM AF Wiederoder, Michael S. Liu, Nancy (Tong) Lefcourt, Alan M. Kim, Moon S. Lo, Y. Martin TI Use of a portable hyperspectral imaging system for monitoring the efficacy of sanitation procedures in produce processing plants SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Hyperspectral imaging; Food safety; Sanitation inspection; Portable; Produce ID PRIMARY-CELL WALL; FECAL CONTAMINATION; PHENOLIC COMPONENTS; UNITED-STATES; BIOFILMS; IDENTIFICATION; SURFACES; APPLES; FOODS AB Cleaning and sanitation of production surfaces and equipment plays a critical role in lowering the risk of food borne illness associated with consumption of fresh-cut produce. Visual observation and sampling methods including ATP tests and cell culturing are commonly used to monitor the effectiveness of the cleaning procedures. This study tested the ability of a hand-held VIS hyperspectral imaging system to augment current monitoring methods. Multiple visits were made to two commercial fresh-cut processing facilities. Fluorescence-based detection and automated cycling among three wavelengths, 475, 520, and 675 nm, proved best for detecting a range of anomalies. Numerous deficiencies in existing cleaning protocols were identified. Plant personnel were able to devise changes in the protocols that eliminated most of the detected problems without increasing the net expenditure for cleaning efforts. In addition, imaging identified sites where ATP test results were higher when compared to results for adjacent areas. Published by Elsevier Ltd. C1 [Wiederoder, Michael S.; Liu, Nancy (Tong); Lefcourt, Alan M.; Kim, Moon S.] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Wiederoder, Michael S.; Liu, Nancy (Tong); Lo, Y. Martin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20740 USA. RP Lefcourt, AM (reprint author), ARS, USDA, Environm Microbial & Food Safety Lab, Bldg 303, Beltsville, MD 20705 USA. EM alan.lefcourt@ars.usda.gov OI Wiederoder, Michael/0000-0003-2294-7142 FU USDA; Department of Nutrition and Food Science, University of Maryland, College Park FX This work was funded by the USDA. Partial support for Michael S. Wiederoder and Nancy Liu was provided by the Department of Nutrition and Food Science, University of Maryland, College Park. The USDA is an equal opportunity employer. NR 27 TC 7 Z9 7 U1 3 U2 32 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 J9 J FOOD ENG JI J. Food Eng. PD JUL PY 2013 VL 117 IS 2 BP 217 EP 226 DI 10.1016/j.jfoodeng.2013.02.019 PG 10 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 133KI UT WOS:000318136900006 ER PT J AU Lloyd, W Pallickara, S David, O Lyon, J Arabi, M Rojas, K AF Lloyd, W. Pallickara, S. David, O. Lyon, J. Arabi, M. Rojas, K. TI Performance implications of multi-tier application deployments on Infrastructure-as-a-Service clouds: Towards performance modeling SO FUTURE GENERATION COMPUTER SYSTEMS-THE INTERNATIONAL JOURNAL OF GRID COMPUTING AND ESCIENCE LA English DT Article DE Service isolation; Infrastructure-as-a-Service; Provisioning variation; Virtualization ID ISSUES AB Hosting a multi-tier application using an Infrastructure-as-a-Service (IaaS) cloud requires deploying components of the application stack across virtual machines (VMs) to provide the application's infrastructure while considering factors such as scalability, fault tolerance, performance and deployment costs (# of VMs). This paper presents results from an empirical study which investigates implications for application performance and resource requirements (CPU, disk and network) resulting from how multi-tier applications are deployed to IaaS clouds. We investigate the implications of: (1) component placement across VMs, (2) VM memory size, (3) VM hypervisor type (KVM vs. Xen), and (4) VM placement across physical hosts (provisioning variation). All possible deployment configurations for two multi-tier application variants are tested. One application variant was computationally bound by the application middleware, the other bound by geospatial queries. The best performing deployments required as few as 2 VMs, half the number required for VM-level service isolation, demonstrating potential cost savings when components can be consolidated. Resource utilization (CPU time, disk I/O, and network I/O) varied with component deployment location, VM memory allocation, and the hypervisor used (Xen or KVM) demonstrating how application deployment decisions impact required resources. Isolating application components using separate VMs produced performance overhead of similar to 1%-2%. Provisioning variation of VMs across physical hosts produced overhead up to 3%. Relationships between resource utilization and performance were assessed using multiple linear regression to develop a model to predict application deployment performance. Our model explained over 84% of the variance and predicted application performance with mean absolute error of only similar to 0.3 s with CPU time, disk sector reads, and disk sector writes serving as the most powerful predictors of application performance. (c) 2012 Elsevier B.V. All rights reserved. C1 [Lloyd, W.; Pallickara, S.; David, O.] Colorado State Univ, Dept Comp Sci, Ft Collins, CO 80523 USA. [Lloyd, W.; David, O.; Lyon, J.; Arabi, M.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Rojas, K.] USDA, NRCS, Ft Collins, CO 80526 USA. RP Lloyd, W (reprint author), ARS, USDA, ASRU, 2150 Ctr Ave,Bldg D,Suite 200, Ft Collins, CO 80526 USA. EM wlloyd@acm.org NR 36 TC 7 Z9 8 U1 0 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-739X J9 FUTURE GENER COMP SY JI Futur. Gener. Comp. Syst. PD JUL PY 2013 VL 29 IS 5 BP 1254 EP 1264 DI 10.1016/j.future.2012.12.007 PG 11 WC Computer Science, Theory & Methods SC Computer Science GA 130CE UT WOS:000317889500013 ER PT J AU Lefcourt, AM Wiederoder, MS Liu, N Kim, MS Lo, M AF Lefcourt, Alan M. Wiederoder, Michael S. Liu, Nancy (Tong) Kim, Moon S. Lo, Martin TI Development of a portable hyperspectral imaging system for monitoring the efficacy of sanitation procedures in food processing facilities SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Hyperspectral imaging; Fluorescence imaging; Food safety; Sanitation inspection; Portable ID CONTAMINATION; ATP AB Cleaning and sanitation in food processing facilities is a critical step in reducing the risk of transfer of pathogenic organisms to food consumed by the public. Current methods to check the effectiveness of sanitation procedures rely on visual observation and sub-sampling tests such as ATP bioluminescence assays and culturing methods. To augment existing verification methods, a hand-held visible hyperspectral imaging device was developed. The device is capable of acquiring reflectance images using ambient lighting, and fluorescence responses to supplemental violet (405 nm) excitation. To enhance the ability of detecting relatively low intensity fluorescence responses in the presence of ambient lighting, the device includes the ability to identify wavebands where the intensity of ambient lighting is relatively low. Valleys in ambient illumination intensity when using fluorescent lighting were found at around 475, 520, 570, and 675 nm. A principal goal is to acquire data to support development of a commercially-viable, hand-held, imaging system. (c) 2013 Elsevier Ltd. All rights reserved. C1 [Lefcourt, Alan M.; Wiederoder, Michael S.; Liu, Nancy (Tong); Kim, Moon S.] USDA, Agr Res Serv, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Wiederoder, Michael S.; Liu, Nancy (Tong); Lo, Martin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20740 USA. RP Lefcourt, AM (reprint author), USDA, Agr Res Serv, Environm Microbial & Food Safety Lab, Bldg 303, Beltsville, MD 20705 USA. EM alan.lefcourt@ars.usda.gov OI Wiederoder, Michael/0000-0003-2294-7142 FU USDA; Department of Nutrition and Food Science, University of Maryland, College Park FX This work was funded by the USDA. Partial support for Michael S. Wiederodear and Nancy Liu was provided by the Department of Nutrition and Food Science, University of Maryland, College Park. The USDA is an equal opportunity employer. NR 15 TC 4 Z9 4 U1 0 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 J9 J FOOD ENG JI J. Food Eng. PD JUL PY 2013 VL 117 IS 1 BP 59 EP 66 DI 10.1016/j.jfoodeng.2013.01.043 PG 8 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA 130CO UT WOS:000317890500008 ER PT J AU Wang, SY Gao, HY AF Wang, Shiow Y. Gao, Haiyan TI Effect of chitosan-based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria x aranassa Duch.) SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Strawberries; Fragaria x ananassa; Chitosan; Antioxidants; Antioxidant enzymes; Free radicals; beta-1,3-Glucanase ID ASCORBIC-ACID; FRESH STRAWBERRIES; SENSITIVE METHOD; VITAMIN-C; CAPACITY; ASSAY; GLUTATHIONE; PEROXIDASE; METABOLISM; ANTHOCYANINS AB The severity of decay in strawberries stored at either 5 degrees C or 10 degrees C was significantly reduced and the shelf-life was extended by immersing fruits in chitosan solutions of 0.5, 1.0 and 1.5 g/100 mL for 5 min at 20 degrees C as compared to the control. Strawberries treated with chitosan also maintained better fruit quality with higher levels of phenolics, anthocyanins, flavonoids (ellagic acid, ellagic acid glucoside, p-coumaroyl glucose, quercetin 3-glucoside, quercetin 3-glucuronide, kaempferol 3-glucoside, kaempferol 3-glucuronide, cyanidin 3-glucoside, pelargonidin 3-glucoside, cyanidin 3-glucoside-succinate, and pelargonidin 3-glucoside-succinate), antioxidant enzyme activity [catalase (CAT), glutathione-peroxidase (GSH-POD), guaiacol peroxidase (G-POD), dehydroascorbate reductase (DHAR) and monodehydroascorbate reductase (MDAR)], and oxygen radical absorbance capacity for peroxyl radicals (ROO center dot), hydroxyl radical radicals ((OH)-O-center dot; HOSC) and 2,2-Di (4-tert-octylphenyl)-1-picrylhydrazyl (DPPH) than the untreated fruits. Chitosan treatments retarded the decrease of ascorbic acid (ASA) and reduced glutathione (GSH) content and beta-1,3-glucanase activities compared to control. The high contents of antioxidants, antioxidant activity, ASA and GSH and high activity of beta-1,3-glucanase in the treated strawberries reinforced the microbial defense mechanism of the fruit and accentuated the resistance against fungal invasion. Therefore, the application of chitosan coating could be favorable in extending shelf-life, maintaining quality and controlling decay of strawberries. Published by Elsevier Ltd. C1 [Wang, Shiow Y.] ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Gao, Haiyan] ARS, Food Qual Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Gao, Haiyan] Zhejiang Acad Agr Sci, Inst Food Sci, Hangzhou 310021, Zhejiang, Peoples R China. RP Wang, SY (reprint author), ARS, Genet Improvement Fruits & Vegetables Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM shiow.wang@ars.usda.gov NR 50 TC 41 Z9 44 U1 12 U2 147 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JUL PY 2013 VL 52 IS 2 SI SI BP 71 EP 79 DI 10.1016/j.lwt.2012.05.003 PG 9 WC Food Science & Technology SC Food Science & Technology GA 129WO UT WOS:000317874900003 ER PT J AU Lu, RF Ariana, DP AF Lu, Renfu Ariana, Diwan P. TI Detection of fruit fly infestation in pickling cucumbers using a hyperspectral reflectance/transmittance imaging system SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Postharvest quality; Pickling cucumber; Fruit fly; Pest infestation; Hyperspectral imaging; Reflectance; Transmittance; Discriminant analysis AB Fruit fly infestation can be a serious problem in pickling cucumber production. In the United States and many other countries, there is zero tolerance for fruit flies in pickled cucumber products. Currently, processors rely on manual inspection to :detect and remove fruit fly-infested cucumbers, which is labor intensive and also prone to error due to human fatigue and the difficulty of visually detecting infestation that is hidden inside the fruit. In this research, a laboratory hyperspectral imaging system operated in an integrated mode of reflectance and transmittance was used to detect fruit fly-infested pickling cucumbers. Hyperpectral reflectance (450-740 nm) and transmittance (740-1000 nm) images were acquired simultaneously for 329 normal (infestation-free) and fruit fly-infested pickling cucumbers of three size classes with the mean diameters of 16.8, 22.1, and 27.6 mm, respectively. Mean spectra were extracted from the hyperspectral image of each cucumber, and they were then corrected for the fruit size effect using a diameter correction equation. Partial least squares discriminant analyses for the reflectance, transmittance and their combined data were performed for differentiating normal and infested pickling cucumbers. With reflectance mode, the overall classification accuracies for the three size classes and the mixed class were between 82% and 88%, whereas transmittance achieved better classification results with the overall accuracies of 88-93%. Integration of reflectance and transmittance did not result in noticeable improvements, compared to transmittance mode. The hyperspectral imaging system performed better than manual inspection, which had an overall accuracy of 75% and whose performance decreased significantly for smaller size cucumbers. This research demonstrated that hyperspectral imaging is potentially useful for detecting fruit fly-infested pickling cucumbers. Published by Elsevier B.V. C1 [Lu, Renfu] Michigan State Univ, USDA, ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. [Ariana, Diwan P.] Michigan State Univ, Dept Biosyst & Agr Engn, E Lansing, MI 48824 USA. RP Lu, RF (reprint author), Michigan State Univ, USDA, ARS, Sugarbeet & Bean Res Unit, 224 Farrall Hall, E Lansing, MI 48824 USA. EM renfu.lu@ars.usda.gov; diwanariana@gmail.com NR 14 TC 15 Z9 18 U1 3 U2 46 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD JUL PY 2013 VL 81 BP 44 EP 50 DI 10.1016/j.postharvbio.2013.02.003 PG 7 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA 129XY UT WOS:000317878500007 ER PT J AU Liu, ZJ Liu, XE Fei, BH Jiang, ZH Cai, ZY Yu, Y AF Liu, Zhijia Liu, Xing'e Fei, Benhua Jiang, Zehui Cai, Zhiyong Yu, Yan TI The properties of pellets from mixing bamboo and rice straw SO RENEWABLE ENERGY LA English DT Article DE Biomass; Bioenergy; Bamboo; Rice straw; Pellet ID PHYSICAL-PROPERTIES; MOISTURE-CONTENT; WOOD; BIOMASS; COMBUSTION; PRODUCTS; FUEL AB Rice straw pellets are the main type of biomass solid fuel and have great potential as a bioenergy resource of the future in China. But it also showed important problems because of its high content of ashes and its low gross calorific value, reducing the possibility to be used in domestic heating. It was certified that mixing different types of biomass materials was helpful to improve the properties of pellets. To improve properties of rice straw pellets and investigate the effect of mixing bamboo and rice straw on the pellet properties, some properties of pellets, manufactured using different mixing ratio of bamboo and rice straw particles, were determined in this research. It can be concluded from this research that physical properties of all pellets meet the requirements of Pellet Fuels Institute Standard Specification for Residential/Commercial Densified except for bulk density of pellets, manufactured using mixing ratio (<= 3:2) of bamboo and rice straw. The inorganic ash and gross calorific value of rice straw pellets cannot meet the requirement of Pellet Fuels Institute Standard Specification for Residential/Commercial Densified (8.0%) and the minimum requirement for making commercial pellets of DIN 51731 (>17,500 J/g). Both properties are improved through mixing bamboo particles and rice straw particles. It is significant that inorganic ash content and gross calorific value of pellets, manufactured using mixing ratio (>= 3:2) of bamboo and rice straw, were lower than 8.0% and higher than 17,500 J/g, respectively. This also shows that mixing different biomass materials is an effective way to optimize properties of biomass solid fuel. All pellets after improvement are proposed as biomass solid fuel and have the potential to be developed as commercial pellets on an industrial scale in China. Crown Copyright (C) 2012 Published by Elsevier Ltd. All rights reserved. C1 [Liu, Zhijia; Liu, Xing'e; Fei, Benhua; Jiang, Zehui; Yu, Yan] Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. [Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. RP Liu, XE (reprint author), Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. EM zhijia_liu@163.com; liuxe@icbr.ac.cn; Feibenhua@icbr.ac.cn RI Liu, Xiaobing/C-6216-2013 OI Liu, Xiaobing/0000-0002-5347-5566 FU Basic Scientific Research Funds of International Centre for Bamboo and Rattan [1632012002]; 12th Five Years Plan-Study on manufacturing technology of functional bamboo (rattan)-based materials [2012BAD54G01] FX This research was financially supported by the 'Basic Scientific Research Funds of International Centre for Bamboo and Rattan' (Grant No. 1632012002) and '12th Five Years Plan-Study on manufacturing technology of functional bamboo (rattan)-based materials' (Grant No. 2012BAD54G01). NR 31 TC 16 Z9 16 U1 5 U2 78 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD JUL PY 2013 VL 55 BP 1 EP 5 DI 10.1016/j.renene.2012.12.014 PG 5 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA 111QN UT WOS:000316535900001 ER PT J AU Beckman, J Xiarchos, IM AF Beckman, Jayson Xiarchos, Irene M. TI Why are Californian farmers adopting more (and larger) renewable energy operations? SO RENEWABLE ENERGY LA English DT Article DE Renewable energy; Double hurdle; Agriculture; Technology adoption ID PROBIT COEFFICIENTS; CONSERVATION; SYSTEMS; POLICIES; MODELS; STATES; LOGIT AB The paper examines the renewable energy adoption and system size determinants for Californian farmers. We utilize a double-hurdle methodology where system size is determined through a hetero-skedastic ordered binary variable model for four size categories. Our results for system size show that determinants of technology adoption differ from size determinants. For example, environmental practices, Internet connection, and electricity price influence adoption, while, total value of production and acre value impact the size of the installed system. Surprisingly, the price of electricity bears no impact on the size category chosen. Renewable energy adoption and system size have been increasing over time, thus our insights about the interplay of the determinants for adoption and size choice, will assist policy formation. Published by Elsevier Ltd. C1 [Beckman, Jayson; Xiarchos, Irene M.] USDA, Econ Res Serv, Washington, DC 20036 USA. [Xiarchos, Irene M.] USDA, Off Energy Policy & New Uses, Off Chief Economist, Washington, DC 20250 USA. RP Xiarchos, IM (reprint author), USDA, Off Energy Policy & New Uses, Off Chief Economist, 1400 Independence Ave,SW,South Bldg, Washington, DC 20250 USA. EM imxiarchos@gmail.com NR 33 TC 7 Z9 7 U1 0 U2 30 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD JUL PY 2013 VL 55 BP 322 EP 330 DI 10.1016/j.renene.2012.10.057 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA 111QN UT WOS:000316535900036 ER PT J AU Huang, LH AF Huang, Lihan TI Optimization of a new mathematical model for bacterial growth SO FOOD CONTROL LA English DT Article DE Primary models; Numerical analysis; Modeling ID KINETICS; BEEF; FOOD AB The objective of this work was to optimize a mathematical equation for use as a primary kinetic model that employed a new approach to describe the three-phase growth of bacteria under constant temperature conditions. This research adopted an optimization algorithm in combination with the Runge-Kutta method to solve the differential form of the new growth model in search of an optimized lag phase transition coefficient (LPTC), which is used to define the adaption and duration of lag phases of bacteria prior to exponential growth. Growth curves of Listeria monocytogenes, Escherichia coli O157:H7, and Clostridium perfringens, selected from previously published data, were analyzed to obtain an optimized LPTC for each growth curve and a global LPTC for all growth curves. With the new optimized LPTC, the new growth model could be used to accurately describe the bacterial growth curves with three distinctive phases (lag, exponential, and stationary). The new optimized LPTC significantly improved the performance and applicability of the new model. The results of statistical analysis (ANOVA) suggested that the new growth model performed equally well with the Baranyi model. It can be used as an alternative primary model for bacterial growth if the bacterial adaption is more significant in controlling the lag phase development. Published by Elsevier Ltd. C1 ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Huang, LH (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM lihan.huang@ars.usda.gov NR 7 TC 16 Z9 19 U1 2 U2 63 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JUL PY 2013 VL 32 IS 1 BP 283 EP 288 DI 10.1016/j.foodcont.2012.11.019 PG 6 WC Food Science & Technology SC Food Science & Technology GA 106SC UT WOS:000316164000043 ER PT J AU Britch, SC Binepal, YS Ruder, MG Kariithi, HM Linthicum, KJ Anyamba, A Small, JL Tucker, CJ Ateya, LO Oriko, AA Gacheru, S Wilson, WC AF Britch, Seth C. Binepal, Yatinder S. Ruder, Mark G. Kariithi, Henry M. Linthicum, Kenneth J. Anyamba, Assaf Small, Jennifer L. Tucker, Compton J. Ateya, Leonard O. Oriko, Abuu A. Gacheru, Stephen Wilson, William C. TI Rift Valley Fever Risk Map Model and Seroprevalence in Selected Wild Ungulates and Camels from Kenya SO PLOS ONE LA English DT Article ID SAUDI-ARABIA; VERTICAL TRANSMISSION; VEGETATION INDEX; AFRICAN BUFFALO; VIRUS; OUTBREAK; ANTIBODY; HUMANS; PREVALENCE; PREVENTION AB Since the first isolation of Rift Valley fever virus (RVFV) in the 1930s, there have been multiple epizootics and epidemics in animals and humans in sub-Saharan Africa. Prospective climate-based models have recently been developed that flag areas at risk of RVFV transmission in endemic regions based on key environmental indicators that precede Rift Valley fever (RVF) epizootics and epidemics. Although the timing and locations of human case data from the 2006-2007 RVF outbreak in Kenya have been compared to risk zones flagged by the model, seroprevalence of RVF antibodies in wildlife has not yet been analyzed in light of temporal and spatial predictions of RVF activity. Primarily wild ungulate serum samples from periods before, during, and after the 2006-2007 RVF epizootic were analyzed for the presence of RVFV IgM and/or IgG antibody. Results show an increase in RVF seropositivity from samples collected in 2007 (31.8%), compared to antibody prevalence observed from 2000-2006 (3.3%). After the epizootic, average RVF seropositivity diminished to 5% in samples collected from 2008-2009. Overlaying maps of modeled RVF risk assessments with sampling locations indicated positive RVF serology in several species of wild ungulate in or near areas flagged as being at risk for RVF. Our results establish the need to continue and expand sero-surveillance of wildlife species Kenya and elsewhere in the Horn of Africa to further calibrate and improve the RVF risk model, and better understand the dynamics of RVFV transmission. C1 [Britch, Seth C.; Linthicum, Kenneth J.] ARS, Ctr Med Agr & Vet Entomol, USDA, Gainesville, FL USA. [Binepal, Yatinder S.; Kariithi, Henry M.; Ateya, Leonard O.; Oriko, Abuu A.] Kenya Agr Res Inst, Ctr Biotechnol, Nairobi, Kenya. [Ruder, Mark G.; Wilson, William C.] ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. [Anyamba, Assaf; Small, Jennifer L.; Tucker, Compton J.] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Gacheru, Stephen] Vet Serv, Kabete, Kenya. RP Wilson, WC (reprint author), ARS, Arthropod Borne Anim Dis Res Unit, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. EM william.wilson@ars.usda.gov FU International Atomic Energy Agency (IAEA); US Department of Agriculture, Agricultural Research Service Project [58-5430-005-00D]; Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-07-00982]; Department of Defense-Armed Forces Health Surveillance Center; United States Department of Agriculture -Agricultural Research Service, Division of GEIS Operations FX We are indebted to the International Atomic Energy Agency (IAEA) for the initial funding of this study. This project was also support in part by the US Department of Agriculture, Agricultural Research Service Project #58-5430-005-00D through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-07-00982. The Department of Defense-Armed Forces Health Surveillance Center, Division of GEIS Operations and the United States Department of Agriculture -Agricultural Research Service supported the RVF Monitoring and Risk Mapping project. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 52 TC 20 Z9 24 U1 2 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 28 PY 2013 VL 8 IS 6 AR e66626 DI 10.1371/journal.pone.0066626 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 174IH UT WOS:000321148400018 PM 23840512 ER PT J AU Cross, BM Hack, A Reinhardt, TA Rao, R AF Cross, Brandie M. Hack, Anniesha Reinhardt, Timothy A. Rao, Rajini TI SPCA2 Regulates Orai1 Trafficking and Store Independent Ca2+ Entry in a Model of Lactation SO PLOS ONE LA English DT Article ID MAMMARY-GLAND; CALCIUM-TRANSPORT; PLASMA-MEMBRANE; EXPRESSION; CELLS; LOCALIZATION; CA2+-ATPASES; TISSUE; TUMORS; MILK AB An unconventional interaction between SPCA2, an isoform of the Golgi secretory pathway Ca2+-ATPase, and the Ca2+ influx channel Orai1, has previously been shown to contribute to elevated Ca2+ influx in breast cancer derived cells. In order to investigate the physiological role of this interaction, we examined expression and localization of SPCA2 and Orai1 in mouse lactating mammary glands. We observed co-induction and co-immunoprecipitation of both proteins, and isoform-specific differences in the localization of SPCA1 and SPCA2. Three-dimensional cultures of normal mouse mammary epithelial cells were established using lactogenic hormones and basement membrane. The mammospheres displayed elevated Ca2+ influx by store independent mechanisms, consistent with upregulation of both SPCA2 and Orai1. Knockdown of either SPCA2 or Orai1 severely depleted Ca2+ influx and interfered with mammosphere differentiation. We show that SPCA2 is required for plasma membrane trafficking of Orai1 in mouse mammary epithelial cells and that this function can be replaced, at least in part, by a membrane-anchored C-terminal domain of SPCA2. These findings clearly show that SPCA2 and Orai1 function together to regulate Store-independent Ca2+ entry (SICE), which mediates the massive basolateral Ca2+ influx into mammary epithelia to support the large calcium transport requirements for milk secretion. C1 [Cross, Brandie M.; Hack, Anniesha; Rao, Rajini] Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21205 USA. [Reinhardt, Timothy A.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. RP Rao, R (reprint author), Johns Hopkins Univ, Sch Med, Dept Physiol, Baltimore, MD 21205 USA. EM rrao@jhmi.edu RI Reinhardt, Timothy/A-7536-2009 OI Reinhardt, Timothy/0000-0001-5552-2509 FU National Institutes of Health [GM62142]; United States Department of Agriculture Agricultural Research Service intramural funding CRIS [3625-32000-102-00D] FX This work was supported by a grant from the National Institutes of Health GM62142 (to RR) and United States Department of Agriculture Agricultural Research Service intramural funding CRIS 3625-32000-102-00D to (TR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 22 TC 15 Z9 15 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 28 PY 2013 VL 8 IS 6 AR e67348 DI 10.1371/journal.pone.0067348 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 174IH UT WOS:000321148400060 PM 23840669 ER PT J AU Lacombe, A Li, RW Klimis-Zacas, D Kristo, AS Tadepalli, S Krauss, E Young, R Wu, VCH AF Lacombe, Alison Li, Robert W. Klimis-Zacas, Dorothy Kristo, Aleksandra S. Tadepalli, Shravani Krauss, Emily Young, Ryan Wu, Vivian C. H. TI Lowbush Wild Blueberries Have the Potential to Modify Gut Microbiota and Xenobiotic Metabolism in the Rat Colon SO PLOS ONE LA English DT Article ID DIET VACCINIUM-ANGUSTIFOLIUM; DEXTRAN SULFATE SODIUM; POLYPHENOLS; BIOTECHNOLOGY; ANTHOCYANINS; IMPROVEMENTS; MICROFLORA; COMPONENTS; PROBIOTICS; SEVERITY AB The gastrointestinal tract is populated by an array of microbial species that play an important role in metabolic and immune functions. The composition of microorganisms is influenced by the components of the host's diet and can impact health. In the present study, dietary enrichment of lowbush wild blueberries (LWB) was examined to determine their effect on colon microbial composition and their potential in promoting gut health. The microbial composition and functional potential of the colon microbiota from Sprague Dawley rats fed control diets (AIN93) and LWB-enriched diets (AIN93+8% LWB powder substituting for dextrose) for 6 weeks were assessed using Illumina shotgun sequencing and bioinformatics tools. Our analysis revealed an alteration in the relative abundance of 3 phyla and 22 genera as representing approximately 14 and 8% of all phyla and genera identified, respectively. The LWB-enriched diet resulted in a significant reduction in the relative abundance of the genera Lactobacillus and Enterococcus. In addition, hierarchal analysis revealed a significant increase in the relative abundance of the phylum Actinobacteria, the order Actinomycetales, and several novel genera under the family Bifidobacteriaceae and Coriobacteriaceae, in the LWB group. Functional annotation of the shotgun sequences suggested that approximately 9% of the 4709 Kyoto Encyclopaedia of Gene and Genome (KEGG) hits identified were impacted by the LWB-diet. Open Reading Frames (ORFs) assigned to KEGG category xenobiotics biodegradation and metabolism were significantly greater in the LWB-enriched diet compared to the control and included the pathway for benzoate degradation [PATH:ko00362] and glycosaminoglycan degradation [PATH:ko00531]. Moreover, the number of ORFs assigned to the bacterial invasion of epithelial cells [PATH:ko05100] pathway was approximately 8 fold lower in the LWB group compared to controls. This study demonstrated that LWBs have the potential to promote gut health and can aid in the development of optimal diets. C1 [Lacombe, Alison; Klimis-Zacas, Dorothy; Kristo, Aleksandra S.; Tadepalli, Shravani; Krauss, Emily; Wu, Vivian C. H.] Univ Maine, Dept Food Sci & Human Nutr, Orono, ME USA. [Li, Robert W.] ARS, USDA, BARC, Bovine Funct Genom Lab, Beltsville, MD USA. [Young, Ryan] Univ Maine, Dept Mol & Biomed Sci, Orono, ME USA. RP Wu, VCH (reprint author), Univ Maine, Dept Food Sci & Human Nutr, Orono, ME USA. EM vivian.wu@umit.maine.edu FU USDA ARS, and USDA National Needs Graduate Fellowship [2007-38420-17764]; UMaine Center for Excellence in Teaching and Assessment Active Student Learning Micro-Grants; Maine Agricultural and Forest Experiment Station at the University of Maine; Hatch from the USDA National Institute of Food and Agriculture [ME08562-10] FX This research was supported by USDA ARS, and USDA National Needs Graduate Fellowship Competitive (award #:2007-38420-17764), UMaine Center for Excellence in Teaching and Assessment Active Student Learning Micro-Grants, and the Maine Agricultural and Forest Experiment Station at the University of Maine. This work is based upon research supported in part by Hatch (Grant number ME08562-10) from the USDA National Institute of Food and Agriculture. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 64 TC 10 Z9 11 U1 2 U2 40 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 28 PY 2013 VL 8 IS 6 AR e67497 DI 10.1371/journal.pone.0067497 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 174IH UT WOS:000321148400092 PM 23840722 ER PT J AU Pridgeon, JW Klesius, PH Song, L Zhang, DH Kojima, K Mobley, JA AF Pridgeon, Julia W. Klesius, Phillip H. Song, Lin Zhang, Dunhua Kojima, Kyoko Mobley, James A. TI Identification, virulence, and mass spectrometry of toxic ECP fractions of West Alabama isolates of Aeromonas hydrophila obtained from a 2010 disease outbreak SO VETERINARY MICROBIOLOGY LA English DT Article DE Aeromonas hydrophila; Identification; Virulence; Extracellular product; Mass spectrometry; Virulence factor ID EXTRACELLULAR PRODUCTS; CHANNEL CATFISH; INFECTION; GENES; MOUSE AB In West Alabama, disease outbreaks in 2009 caused by Aeromonas hydrophila have led to an estimated loss of more than $3 million. In 2010, disease outbreak occurred again in West Alabama, causing losses of hundreds of thousands of pounds of market size channel catfish. During the 2010 disease outbreak in West Alabama, four isolates of A. hydrophila were cultured from the kidney tissues of diseased channel catfish. Both analytical profile index (API) 20 E biochemical tests and 16S-23S rRNA sequencing results confirmed the four isolates as A. hydrophila. Virulence studies revealed that the four isolates were highly virulent to channel catfish by intraperitoneal injection, with LD50 value of similar to 1.3 x 10(5) CFU/fish. Extracellular proteins (ECPs) of A. hydrophila are well known to be toxic to fish. Therefore, ECPs of the four 2010 West Alabama isolates of A. hydrophila were characterized in this study. The ECPs of the four 2010 isolates were found to be toxic to channel catfish fingerlings, with LD50 value of 16 mu g/fish. Thirty ECP fractions were obtained from the ECPs of the 2010 isolates of A. hydrophila by cation-exchange chromatography, of which nine fractions were found to be toxic to catfish gill cells and channel catfish fingerlings. Mass spectrometry identified 228 proteins from the nine toxic fractions, of which 23 were shared by toxic fractions, including well known virulence factors such as hemolysin, aerolysin, elastase (metalloprotease), nuclease, and 5'-nucleotidase. Hemolytic activity, protease activity, and nuclease activity of the four isolates were found to be significantly (P < 0.05) higher than that of a reference A. hydrophila strain AL98-C1B. Our results might shed light on the possible virulence factors of the highly virulent West Alabama isolates of A. hydrophila. Published by Elsevier B.V. C1 [Pridgeon, Julia W.; Klesius, Phillip H.; Song, Lin; Zhang, Dunhua] ARS, Aquat Anim Hlth Res Unit, USDA, Auburn, AL 36830 USA. [Kojima, Kyoko; Mobley, James A.] Univ Alabama Birmingham, UAB Bioanalyt & Mass Spectrometry Shared Facil, Dept Surg, Birmingham, AL 35294 USA. RP Pridgeon, JW (reprint author), Aquat Anim Hlth Res Unit, 990 Wire Rd, Auburn, AL 36832 USA. EM Julia.Pridgeon@ars.usda.gov FU USDA/ARS CRIS [6420-32000-024-00D] FX We thank Drs. Mediha Aksoy (USDA-ARS) and Victor Panangala (USDA collaborator) for critical reviews of the manuscript. We thank Bill Hemstreet and Julie Bebak for obtaining the ML-10-51 K strain. We thank Beth Peterman, Alberta Pink, and Julio Garcia (USDA-ARS) for their technical support. We thank Dr. Brian Scheffler and Fanny Liu (USDA-ARS) for their excellent sequencing service. We also thank the management team of the Aquatic Animal Health Research Unit for daily care and management of the fish. This study was supported by the USDA/ARS CRIS project #6420-32000-024-00D. The use of trade, firm, or corporate names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. NR 27 TC 8 Z9 10 U1 0 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 J9 VET MICROBIOL JI Vet. Microbiol. PD JUN 28 PY 2013 VL 164 IS 3-4 BP 336 EP 343 DI 10.1016/j.vetmic.2013.02.020 PG 8 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA 157KG UT WOS:000319894400018 PM 23523171 ER PT J AU Zheng, JS Arnett, DK Parnell, LD Lee, YC Ma, YY Smith, CE Richardson, K Li, D Borecki, IB Tucker, KL Ordovas, JM Lai, CQ AF Zheng, Ju-Sheng Arnett, Donna K. Parnell, Laurence D. Lee, Yu-Chi Ma, Yiyi Smith, Caren E. Richardson, Kris Li, Duo Borecki, Ingrid B. Tucker, Katherine L. Ordovas, Jose M. Lai, Chao-Qiang TI Polyunsaturated Fatty Acids Modulate the Association between PIK3CA-KCNMB3 Genetic Variants and Insulin Resistance SO PLOS ONE LA English DT Article ID FOOD FREQUENCY QUESTIONNAIRE; PANCREATIC BETA-CELLS; BK CHANNELS; GLUCOSE-HOMEOSTASIS; FISH CONSUMPTION; METABOLIC-REGULATION; PUERTO-RICAN; DIETARY-FAT; PPAR-GAMMA; SENSITIVITY AB Background: Neighboring genes PIK3CA and KCNMB3 are both important for insulin signaling and beta-cell function, but their associations with glucose-related traits are unclear. Objective: The objective was to examine associations of PIK3CA-KCNMB3 variants with glucose-related traits and potential interaction with dietary fat. Design: We first investigated genetic associations and their modulation by dietary fat in the Genetics of Lipid Lowering Drugs and Diet Network (GOLDN) study (n = 820). Nine single-nucleotide polymorphisms (SNPs) were selected for analysis, covering more than 80% of the SNPs in the region. We then sought to replicate the findings in the Boston Puerto Rican Health Study (BPRHS) (n = 844). Results: For KCNMB3 missense mutation rs7645550, meta-analysis indicated that homeostasis model assessment of insulin resistance (HOMA-IR) was significantly lower in minor allele T homozygotes compared with major allele C carriers (pooled P-value = 0.004); for another SNP rs1183319, which is in moderate LD with rs7645550, minor allele G carriers had higher HOMA-IR compared with non-carriers in both populations (pooled P-value = 0.028). In GOLDN, rs7645550 T allele homozygotes had lower HOMA-IR only when dietary n-3: n-6 PUFA ratio was low (<= 0.11, P = 0.001), but not when it was high (. 0.11, P-interaction = 0.033). Similar interaction was observed between rs1183319 and n-3: n-6 PUFA ratio on HOMAIR (P-interaction = 0.001) in GOLDN. Variance contribution analyses in GOLDN confirmed the genetic association and gene-diet interaction. In BPRHS, dietary n-3: n-6 PUFA ratio significantly modulated the association between rs1183319 and HbA1c (P-interaction = 0.034). Conclusion: PIK3CA-KCNMB3 variants are associated with insulin resistance in populations of different ancestries, and are modified by dietary PUFA. C1 [Zheng, Ju-Sheng; Li, Duo] Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310003, Zhejiang, Peoples R China. [Zheng, Ju-Sheng; Parnell, Laurence D.; Lee, Yu-Chi; Ma, Yiyi; Smith, Caren E.; Richardson, Kris; Ordovas, Jose M.; Lai, Chao-Qiang] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Tucker, Katherine L.] Northeastern Univ, Dept Hlth Sci, Boston, MA 02115 USA. RP Li, D (reprint author), Zhejiang Univ, Dept Food Sci & Nutr, Hangzhou 310003, Zhejiang, Peoples R China. EM duoli@zju.edu.cn; chaoqiang.lai@ars.usda.gov OI Zheng, Ju-Sheng/0000-0001-6560-4890; Tucker, Katherine/0000-0001-7640-662X FU China Scholarship Council; National Basic Research Program of China (973 Program) [2011CB504002]; National Heart, Lung, and Blood Institute [HL54776, HL078885]; US Department of Agriculture Research Service [53-K06-5-10, 58-1950-9-001] FX This study was funded by the China Scholarship Council; by the National Basic Research Program of China (973 Program: 2011CB504002); by National Heart, Lung, and Blood Institute grant number HL54776 and HL078885; by the contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 0 Z9 0 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 27 PY 2013 VL 8 IS 6 AR e67394 DI 10.1371/journal.pone.0067394 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 174IX UT WOS:000321150000072 PM 23826284 ER PT J AU Kiefer, MT Zhong, S Heilman, WE Charney, JJ Bian, X AF Kiefer, M. T. Zhong, S. Heilman, W. E. Charney, J. J. Bian, X. TI Evaluation of an ARPS-based canopy flow modeling system for use in future operational smoke prediction efforts SO JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES LA English DT Article DE vegetation canopy; atmospheric model; model evaluation; CHATS; smoke prediction ID LARGE-EDDY SIMULATION; PLANT CANOPIES; TURBULENT-FLOW; FOREST CANOPY; AIR-FLOW; 3-DIMENSIONAL CANOPY; 2ND-ORDER CLOSURE; CHATS EXPERIMENT; SOLAR-RADIATION; LAYER AB Efforts to develop a canopy flow modeling system based on the Advanced Regional Prediction System (ARPS) model are discussed. The standard version of ARPS is modified to account for the effect of drag forces on mean and turbulent flow through a vegetation canopy, via production and sink terms in the momentum and subgrid-scale turbulent kinetic energy (TKE) equations. Additionally, a downward decaying net radiation profile inside the canopy is used to account for the attenuation of net radiation by vegetation elements. As a critical step in the model development process, simulations performed with the new canopy model, termed ARPS-CANOPY, are examined and compared to observations from the Canopy Horizontal Array Turbulence Study (CHATS) experiment. Comparisons of mean and turbulent flow properties in a statistically homogeneous atmosphere are presented for two cases, one when the trees are dormant without leaves and another when the trees are full of mature leaves. The model is shown to reproduce the shape of the vertical profiles of mean wind, temperature, and TKE observed during the CHATS experiment, with errors generally smaller in the afternoon and in the case with stronger mean flow. Sensitivity experiments with relatively coarse (90 m) horizontal grid spacing retain the overall mean profile shapes and diurnal trends seen in the finer-resolution simulations. The work described herein is part of a larger effort to develop predictive tools for close-range (on the order of 1km from the source) smoke dispersion from low-intensity fires within forested areas. C1 [Kiefer, M. T.; Zhong, S.] Michigan State Univ, Dept Geog, E Lansing, MI 48823 USA. [Heilman, W. E.; Charney, J. J.; Bian, X.] US Forest Serv, No Res Stn, USDA, E Lansing, MI USA. RP Kiefer, MT (reprint author), Michigan State Univ, Dept Geog, 1407 S Harrison Rd,Rm 220, E Lansing, MI 48823 USA. EM mtkiefer@msu.edu OI Kiefer, Michael/0000-0002-4824-0148 FU U.S. Joint Fire Science Program [09-1-04-1]; USDA Forest Service [09-JV-11242306-089] FX We wish to thank Jehan Rihani and three anonymous reviewers for their helpful comments and suggestions regarding the manuscript. Support for this research was provided by the U.S. Joint Fire Science Program (project 09-1-04-1) and the USDA Forest Service (Research Joint Venture agreement 09-JV-11242306-089). NR 45 TC 5 Z9 5 U1 1 U2 9 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-897X J9 J GEOPHYS RES-ATMOS JI J. Geophys. Res.-Atmos. PD JUN 27 PY 2013 VL 118 IS 12 BP 6175 EP 6188 DI 10.1002/jgrd.50491 PG 14 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA 187OY UT WOS:000322129600019 ER PT J AU Zhou, HF Lou, HM Yang, DJ Zhu, JY Qiu, XQ AF Zhou, Haifeng Lou, Hongming Yang, Dongjie Zhu, J. Y. Qiu, Xueqing TI Lignosulfonate To Enhance Enzymatic Saccharification of Lignocelluloses: Role of Molecular Weight and Substrate Lignin SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article ID TITER ETHANOL-PRODUCTION; GLOBULAR-PROTEINS; LODGEPOLE PINE; DILUTE-ACID; HYDROLYSIS; INHIBITION; CELLULOSE; SPORL; ASPEN; PRETREATMENTS AB This study conducted an investigation of the effect of lignosulfonate (LS) on enzymatic saccharification of lignocelluloses. Two commercial LSs and one laboratory sulfonated kraft lignin were applied to Whatman paper, dilute acid and SPORL (sulfite pretreatment to overcome recalcitrance of lignocelluloses) pretreated aspen, and kraft alkaline and SPORL pretreated lodgepole pine. All three lignin samples inhibited cellulose saccharification of Whatman paper, but enhanced the saccharification of the four lignocellulosic substrates. The level of enhancement was related to the molecular weight and degree of sulfonation. of the lignin as well as the substrate lignin structure. When different molecular Weight (MW) fractions of one commercial LS (SXP), generated from sulfite pulping of hardwood, were applied to the Whatman paper, the large MW fraction (SXP1) with the lowest degree of sulfonation inhibited cellulose saccharification while the intermediate (SXP2) and smallest (SXP3) MW fractions enhanced saccharification. All MW fractions enhanced saccharification of the four lignocellulosic substrates with maximal enhancement by the smallest MW fraction, SXP3. The enhancement was most significant for the kraft lodgepole pine substrate and least significant for the SPORL pretreated lodgepole pine using all three LS and SXP fractions. The results suggest that LS acts as a surfactant to enhance pure cellullose saccharification. When LS is applied to lignocelluloses, it acts as a surfactant to block bound lignin from binding cellulase nonproductively leading to enhanced saccharification. C1 [Zhou, Haifeng; Lou, Hongming; Yang, Dongjie; Qiu, Xueqing] S China Univ Technol, Sch Chem & Chem Engn, Guangzhou 510006, Guangdong, Peoples R China. [Lou, Hongming; Yang, Dongjie; Qiu, Xueqing] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510006, Guangdong, Peoples R China. [Zhou, Haifeng; Lou, Hongming; Zhu, J. Y.] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Zhu, JY (reprint author), US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM jzhu@fs.fed.us; cexqqiu@scut.edu.cn OI Lou, Hongming/0000-0003-3941-7287 FU USDA Small Business Innovative Research (SBIR) Phase II project [2010-33610-21589]; Chinese Scholarship Council (CSC); International Science and Technology Cooperation Program of China (ISTCP) [2013DFA41670] FX This work was supported by a USDA Small Business Innovative Research (SBIR) Phase II project (Contract No. 2010-33610-21589) to Biopulping International, the Chinese Scholarship Council (CSC), and the International Science and Technology Cooperation Program of China (ISTCP): 2013DFA41670. The funding from these programs made the visiting appointments of H.Z. and H.L. at the USDA Forest Products Laboratory (FPL) possible. We would like to acknowledge Jerry Gargulak of LignoTech for providing us the D748 lignosulfonate, and Fred Matt and Kolby Hirth (both FPL) for conducting carbohydrate and sulfur content measurements, respectively. NR 34 TC 48 Z9 48 U1 3 U2 69 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD JUN 26 PY 2013 VL 52 IS 25 BP 8464 EP 8470 DI 10.1021/ie401085k PG 7 WC Engineering, Chemical SC Engineering GA 175NB UT WOS:000321236300013 ER PT J AU Zhang, JL Zhao, YX Dubay, MR Severtson, SJ Gwin, LE Houtman, CJ AF Zhang, Jilin Zhao, Yuxi Dubay, Matthew R. Severtson, Steven J. Gwin, Larry E. Houtman, Carl J. TI Surface Enrichment by Conventional and Polymerizable Sulfated Nonylphenol Ethoxylate Emulsifiers in Water-Based Pressure-Sensitive Adhesive SO INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH LA English DT Article ID ATOMIC-FORCE MICROSCOPY; LATEX FILMS; REACTIVE SURFACTANTS; HETEROPHASE POLYMERIZATION; MORPHOLOGY; EXUDATION; SURFMERS AB Comparisons of properties are made for pressure-sensitive adhesives (PSAs) generated via emulsion polymerization using both conventional and reactive emulsifiers The emulsifiers are ammonium salts of sulfated nonylphenol ethoxylates with similar chemical structures and hydrophilic lipophilic balances. The polymerizable surfactant possesses a reactive double bond at its phenyl ring allowing for participation in free radical polymerization reactions. Incorporation of the polymerizable surfactant into the adhesive polymer was confirmed via reverse-phase liquid chromatography. Surfactant distributions in PSA films were characterized using confocal Raman microscopy and contact angle measurements. Increasing conventional surfactant concentrations in emulsion polymerization, reactions resulted in proportional increases in concentrations at the surfaces of latex-cast films. No surface enrichment of adhesive films was observed when reactive emulsifiers were employed. Variations in measured depth profiles and contact angles are consistent with observed performance properties, for example, tack, peel strength, and shear times. Increasing concentrations of conventional surfactants decreased adhesive performance, while adhesives generated with polymerizable surfactants showed little or no such decreases in film performance properties. It is concluded that the polymerizable surfactant is bound in the adhesive in such a way that its migration to film surfaces during drying and formation is inhibited, preventing degradation of adhesive performance. C1 [Zhang, Jilin; Zhao, Yuxi; Dubay, Matthew R.; Severtson, Steven J.] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA. [Gwin, Larry E.] Franklin Int, Columbus, OH 43207 USA. [Houtman, Carl J.] USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Severtson, SJ (reprint author), Univ Minnesota, Dept Bioprod & Biosyst Engn, 2004 Folwell Ave, St Paul, MN 55108 USA. EM sever018@umn.edu FU United States Postal Service, Stamp Acquisition and Distribution; Chinese State Key Laboratory of Chemical Engineering at Zhejiang University [SKL-ChE-12D05] FX This project was funded in part by a grant from the United States Postal Service, Stamp Acquisition and Distribution and by the Chinese State Key Laboratory of Chemical Engineering at Zhejiang University (SKL-ChE-12D05). The authors would like to thank Prof. Jonathan Schilling and Justin Kaffenberger for their help in collecting HPLC data. NR 24 TC 6 Z9 6 U1 0 U2 35 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0888-5885 J9 IND ENG CHEM RES JI Ind. Eng. Chem. Res. PD JUN 26 PY 2013 VL 52 IS 25 BP 8616 EP 8621 DI 10.1021/ie401355b PG 6 WC Engineering, Chemical SC Engineering GA 175NB UT WOS:000321236300028 ER PT J AU Carey, AN Fisher, DR Rimando, AM Gomes, SM Bielinski, DF Shukitt-Hale, B AF Carey, Amanda N. Fisher, Derek R. Rimando, Agnes M. Gomes, Stacey M. Bielinski, Donna F. Shukitt-Hale, Barbara TI Stilbenes and Anthocyanins Reduce Stress Signaling in BV-2 Mouse Microglia SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE stilbene; pterostilbene; resveratrol; anthocyanin; blueberry; inflammation; microglia; TNF-alpha ID NF-KAPPA-B; TUMOR-NECROSIS-FACTOR; COLON-CANCER CELLS; LIPID-PEROXIDATION; IN-VITRO; DOWN-REGULATION; NITRIC-OXIDE; PTEROSTILBENE; RESVERATROL; ACTIVATION AB Blueberries contain an array of phytochemicals that may decrease both inflammatory and oxidative stress. This study determined if pterostilbene, resveratrol, and two anthocyanins commonly found in blueberries, delphinidin-3-O-glucoside and malvidin-3-O-glucoside, would be efficacious in protecting microglia from inflammatory induced stress signaling. Microglia that were pretreated with blueberry extract (0.25, 0.5, 1, 2 mg/mL) or its components (1, 10, 20, 30 mu M pterostilbene, resveratrol, delphinidin-3-O-glucoside, or malvidin-3-O-glucoside) prior to exposure to lipopolysaccharide (100 ng/mL) demonstrated concentration dependent reductions in nitric oxide and tumor necrosis factor alpha release and decreased expression of inducible nitric oxide synthase and cyclooxygenase-2. However, much higher concentrations of the individual components than those found in blueberries were needed to demonstrate the effects. For example, 1 mg/mL blueberry extract significantly reduced LPS-induced nitric oxide release; this concentration of blueberry extract contains 2 6 mu M malvidin-3-O-glucoside, but when malvidin-3-O-glucoside was tested individually, 20 mu M was necessary to observe a significant reduction in nitric oxide release. Therefore the protective effects of blueberries may not be due to any one component, but rather a synergism of the activity of the compounds tested and/or other blueberry compounds not tested here. These results lend further support that blueberry and its active components are able to combat some of the inflammatory mediators of aging at the cellular level. C1 [Carey, Amanda N.; Fisher, Derek R.; Gomes, Stacey M.; Bielinski, Donna F.; Shukitt-Hale, Barbara] Tufts Univ, Human Nutr Res Ctr Aging, ARS, USDA, Boston, MA USA. [Carey, Amanda N.] Simmons Coll, Dept Psychol, Boston, MA 02115 USA. [Rimando, Agnes M.] ARS, Natl Ctr Nat Prod Utilizat Res, USDA, University, MS 38677 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, Human Nutr Res Ctr Aging, ARS, USDA, Boston, MA USA. EM barbara.shukitthale@ars.usda.gov FU USDA FX Supported by USDA Intramural funds. NR 38 TC 11 Z9 11 U1 2 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 26 PY 2013 VL 61 IS 25 BP 5979 EP 5986 DI 10.1021/jf400342g PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 175NH UT WOS:000321236900008 PM 23731018 ER PT J AU Khajuria, C Wang, HY Liu, XM Wheeler, S Reese, JC El Bouhssini, M Whitworth, RJ Chen, MS AF Khajuria, Chitvan Wang, Haiyan Liu, Xuming Wheeler, Shanda Reese, John C. El Bouhssini, Mustapha Whitworth, R. Jeff Chen, Ming-Shun TI Mobilization of lipids and fortification of cell wall and cuticle are important in host defense against Hessian fly SO BMC GENOMICS LA English DT Article ID PLANT DEFENSE; MAYETIOLA-DESTRUCTOR; INSECT HERBIVORES; INCOMPATIBLE INTERACTIONS; CECIDOMYIIDAE LARVAE; MICROARRAY ANALYSIS; SIGNALING PATHWAYS; OXIDATIVE BURST; GENE-EXPRESSION; INNATE IMMUNITY AB Background: Wheat - Hessian fly interaction follows a typical gene-for-gene model. Hessian fly larvae die in wheat plants carrying an effective resistance gene, or thrive in susceptible plants that carry no effective resistance gene. Results: Gene sets affected by Hessian fly attack in resistant plants were found to be very different from those in susceptible plants. Differential expression of gene sets was associated with differential accumulation of intermediates in defense pathways. Our results indicated that resources were rapidly mobilized in resistant plants for defense, including extensive membrane remodeling and release of lipids, sugar catabolism, and amino acid transport and degradation. These resources were likely rapidly converted into defense molecules such as oxylipins; toxic proteins including cysteine proteases, inhibitors of digestive enzymes, and lectins; phenolics; and cell wall components. However, toxicity alone does not cause immediate lethality to Hessian fly larvae. Toxic defenses might slow down Hessian fly development and therefore give plants more time for other types of defense to become effective. Conclusion: Our gene expression and metabolic profiling results suggested that remodeling and fortification of cell wall and cuticle by increased deposition of phenolics and enhanced cross-linking were likely to be crucial for insect mortality by depriving Hessian fly larvae of nutrients from host cells. The identification of a large number of genes that were differentially expressed at different time points during compatible and incompatible interactions also provided a foundation for further research on the molecular pathways that lead to wheat resistance and susceptibility to Hessian fly infestation. C1 [Khajuria, Chitvan; Liu, Xuming; Wheeler, Shanda; Reese, John C.; Whitworth, R. Jeff; Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Wang, Haiyan] Kansas State Univ, Dept Stat, Manhattan, KS 66506 USA. [El Bouhssini, Mustapha] Int Ctr Agr Res Dry Area, Aleppo, Syria. [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Chen, MS (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM mchen@ksu.edu FU NIFA grant from the U.S. Department of Agriculture [USDA NIFA 2010-03741] FX The authors want to thank Joshua Beyer for his assistance in Hessian fly rescue assay. Hessian fly voucher specimens (No. 150) are located in the KSU Museum of Entomological and Prairie Arthropod Research, Kansas State University, Manhattan, Kansas. This work was supported by a NIFA grant from the U.S. Department of Agriculture (USDA NIFA 2010-03741). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 63 TC 10 Z9 10 U1 2 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 26 PY 2013 VL 14 AR 423 DI 10.1186/1471-2164-14-423 PG 16 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 177QP UT WOS:000321390600001 PM 23800119 ER PT J AU O'Rourke, JA Iniguez, LP Bucciarelli, B Roessler, J Schmutz, J McClean, PE Jackson, SA Hernandez, G Graham, MA Stupar, RM Vance, CP AF O'Rourke, Jamie A. Iniguez, Luis P. Bucciarelli, Bruna Roessler, Jeffrey Schmutz, Jeremy McClean, Phillip E. Jackson, Scott A. Hernandez, Georgina Graham, Michelle A. Stupar, Robert M. Vance, Carroll P. TI A re-sequencing based assessment of genomic heterogeneity and fast neutron-induced deletions in a common be an cultivar SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Phaseolusvulgaris; commonbean; naturalvariation; structuralvariation; fast neutron mutation; DNA-Seq ID STRUCTURAL VARIATION; ARABIDOPSIS-THALIANA; GENES; PLANT; PROTEINS; MAIZE; MODEL; LINES AB A small fast neutron (FN) mutant population has been established from Phaseolus vulgaris cv. Red Hawk. Weleve ragedthe available P. vulgaris genomes equenceand hight hrough put next generation DNA sequencing to examine the genomic structure of five P. vulgaris cv. Red Hawk FN mutants with striking visual phenotypes. Analysis of these genomes identified three classes of structural variation( SV); between cultivar variation, natural variation within the FN mutant population, and FN induced mutagenesis. Our analyses focused on the latter two classes. We identified 23 large deletions(> 40bp) common to multiple individuals, illustrating residual heterogeneity and regions of SV within the common be ancv. Red Hawk. An additional 18 large deletions were identified in individual mutant plants. These deletions, ranging in size from 40 bpto 43,000 bp, are potentially the result of FN mutagenesis. Six of the 18 deletions lienear or withing enecding regions, identifying potential candidate genes causing the mutant phenotype. C1 [O'Rourke, Jamie A.; Bucciarelli, Bruna; Vance, Carroll P.] USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. [O'Rourke, Jamie A.; Bucciarelli, Bruna; Roessler, Jeffrey; Stupar, Robert M.; Vance, Carroll P.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Iniguez, Luis P.; Hernandez, Georgina] Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62191, Morelos, Mexico. [Schmutz, Jeremy] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [McClean, Phillip E.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58105 USA. [Jackson, Scott A.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Graham, Michelle A.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. RP O'Rourke, JA (reprint author), Univ Minnesota, Plant Sci Res Unit, USDA ARS, 495 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. EM orour119@umn.edu OI Stupar, Robert/0000-0002-8836-2924 NR 31 TC 4 Z9 4 U1 1 U2 13 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 25 PY 2013 VL 4 AR 210 DI 10.3389/fpls.2013.00210 PG 11 WC Plant Sciences SC Plant Sciences GA 296EV UT WOS:000330168800001 PM 23805147 ER PT J AU Silva-Sanchez, C Chen, SX Zhu, N Li, QB Chourey, PS AF Silva-Sanchez, Cecilia Chen, Sixue Zhu, Ning Li, Qin-Bao Chourey, Prem S. TI Proteomic comparison of basal endosperm in maize miniature1 mutant and its wild-type Mn1 SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE proteomics; developing endosperm; iTRAQ; geneontology; maize ID CELL-WALL INVERTASE; PROTEIN DISULFIDE-ISOMERASE; SEED DEVELOPMENT; RESPONSIVE PROTEINS; SUCROSE SYNTHASE; EXPRESSION; PATHWAYS; KERNELS; BIOSYNTHESIS; REVEALS AB Developing endosperm in maize seed is a major site for biosynthesis and storage of starch and proteins, andofimmenseeconomicimportanceforitsroleinfood, feed and biofuel production. The basal part of endosperm performs a major role in solute, water and nutrition acquisition from mother plant to sustain these functions. The miniature 1(mn1) mutation is a loss-of- function mutation of the Mn1-encoded cell wall invertase that is entirely expressed in the basal endosperman dis essential for many of the metabolic and signaling functions associated with metabolically released hexose sugars in developing endosperm. Here were port a comparative proteomic study between Mn1 and mn1 basal endosperm to better understand basis of pleiotropic effects on many diverse traits in the mutant. Specifically, we used iTRAQ based quantitative proteomics combined with Gene ontology( GO) and bioinformatics to understand functional basis of the proteomic information. A total of 2518 proteins were identified from soluble and cell wall associated protein( CWAP) fractions; of these 131 proteins were observed to be differentially expressed in the two genotypes. The main functional groups of proteins that were significantly different were those involved in the carbohydrate metabolic and catabolic process, and cell homeostasis. The study constitutes the first proteomic analysis of basal endosperm cell layers in relation to endosperm growth and development in maize. C1 [Silva-Sanchez, Cecilia; Chen, Sixue] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32610 USA. [Chen, Sixue; Zhu, Ning] Univ Florida, UF Genet Inst, Dept Biol, Gainesville, FL 32610 USA. [Li, Qin-Bao; Chourey, Prem S.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Chourey, Prem S.] Univ Florida, Dept Agron & Plant Pathol, Gainesville, FL 32610 USA. RP Chen, SX (reprint author), Univ Florida, UF Genet Inst, Interdisciplinary Ctr Biotechnol Res, 2033 Mowry Rd,CGRC Rm 438, Gainesville, FL 32610 USA. EM schen@ufl.edu; prem.chourey@ars.usda.gov NR 44 TC 7 Z9 7 U1 0 U2 19 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 25 PY 2013 VL 4 AR 211 DI 10.3389/fpls.2013.00211 PG 16 WC Plant Sciences SC Plant Sciences GA 296ER UT WOS:000330168400001 PM 23805148 ER PT J AU Pierson, JC Allendorf, FW Drapeau, P Schwartz, MK AF Pierson, Jennifer C. Allendorf, Fred W. Drapeau, Pierre Schwartz, Michael K. TI Breed Locally, Disperse Globally: Fine-Scale Genetic Structure Despite Landscape-Scale Panmixia in a Fire-Specialist SO PLOS ONE LA English DT Article ID SEX-BIASED DISPERSAL; SPATIAL AUTOCORRELATION ANALYSIS; BLACK-BACKED WOODPECKERS; POPULATION-DYNAMICS; METAPOPULATIONS; PHILOPATRY; FORESTS; BIRDS; EXTINCTION; FLOW AB An exciting advance in the understanding of metapopulation dynamics has been the investigation of how populations respond to ephemeral patches that go 'extinct' during the lifetime of an individual. Previous research has shown that this scenario leads to genetic homogenization across large spatial scales. However, little is known about fine-scale genetic structuring or how this changes over time in ephemeral patches. We predicted that species that specialize on ephemeral habitats will delay dispersal to exploit natal habitat patches while resources are plentiful and thus display fine-scale structure. To investigate this idea, we evaluated the effect of frequent colonization of ephemeral habitats on the fine-scale genetic structure of a fire specialist, the black-backed woodpecker (Picoides arcticus) and found a pattern of fine-scale genetic structure. We then tested for differences in spatial structure between sexes and detected a pattern consistent with male-biased dispersal. We also detected a temporal increase in relatedness among individuals within newly burned forest patches. Our results indicate that specialist species that outlive their ephemeral patches can accrue significant fine-scale spatial structure that does not necessarily affect spatial structure at larger scales. This highlights the importance of both spatial and temporal scale considerations in both sampling and data interpretation of molecular genetic results. C1 [Pierson, Jennifer C.] Univ Montana, Wildlife Biol Program, Missoula, MT 59812 USA. [Allendorf, Fred W.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Drapeau, Pierre] Univ Quebec, Dept Sci Biol, Ctr Etud Foret, Montreal, PQ H3C 3P8, Canada. [Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT USA. RP Pierson, JC (reprint author), CSIRO Black Mt Site, Canberra, ACT, Australia. EM jencpierson@gmail.com RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU Montana Fish, Wildlife and Parks; Bureau of Land Management; Glacier National Park; Glacier Fund; U.S.D.A. Forest Service; Y2Y Science Grants; Wilburforce Foundation; McIntire-Stennis Cooperative Research Program; National Center for Landscape Fire Analysis; Northwest Scientific Association; Five Valleys Audubon; American Association of University Women; P.E.O. Scholar Award; National Science Foundation; University of Montana; Fonds quebecois de recherche sur la nature et les technologies (FQRNT); Grand Jardins Provincial Park; Natural Science and Engineering Research Council of Canada (NSERC) FX Funding for this project was provided by Montana Fish, Wildlife and Parks, Bureau of Land Management, Glacier National Park, The Glacier Fund, U.S.D.A. Forest Service, Y2Y Science Grants and Wilburforce Foundation, McIntire-Stennis Cooperative Research Program, National Center for Landscape Fire Analysis, Northwest Scientific Association and Five Valleys Audubon. Support for J. Pierson was provided by the American Association of University Women, P.E.O. Scholar Award, National Science Foundation and The University of Montana. Financial support for the Grands Jardins study was provided by the Fonds quebecois de recherche sur la nature et les technologies (FQRNT) - Programme Action concertee - Fonds Forestier, Grand Jardins Provincial Park, and a Natural Science and Engineering Research Council of Canada (NSERC) Discovery Grant (P. Drapeau). The authors received the Alberta Samples from Shawna Pelech. The funders had no role in study design, data collection and analysis, decision to publish, or in preparation of the manuscript. NR 45 TC 9 Z9 9 U1 3 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 25 PY 2013 VL 8 IS 6 AR e67248 DI 10.1371/journal.pone.0067248 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 175IB UT WOS:000321223000092 PM 23825646 ER PT J AU Kallon, S Li, XR Ji, J Chen, CY Xi, QY Chang, S Xue, CY Ma, JY Xie, QM Zhang, YL AF Kallon, Sanpha Li, Xiaorong Ji, Jun Chen, Cuiying Xi, Qianyun Chang, Shuang Xue, Chunyi Ma, Jingyun Xie, Qingmei Zhang, Youngliang TI Astragalus polysaccharide enhances immunity and inhibits H9N2 avian influenza virus in vitro and in vivo SO JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY LA English DT Article DE Astragalus polysaccharide; H9N2 avian influenza virus; Immune effect ID T-LYMPHOCYTE; CHICKEN; VACCINE; RESPONSES AB This study investigated the humoral immunization of Astragalus polysaccharide (APS) against H9N2 avian influenza virus (H9N2 AIV) infection in chickens. The effects of APS treatment on H9N2 infection was evaluated by an MTT [3(4, 5-dimethylthiazol-2-yl)-2, 3-diphenyl tetrazolium bromide] assay and analysis of MHC and cytokine mRNA expression. The effect on lymphocyte and serum antibody titers in vivo was also investigated. IL-4, IL-6, IL-10, LITAF, IL-12 and antibody titers to H9N2 AIV were enhanced in the first week after APS treatment. The results indicated that APS treatment reduces H9N2 AIV replication and promotes early humoral immune responses in young chickens. C1 [Kallon, Sanpha; Li, Xiaorong; Ji, Jun; Chen, Cuiying; Xi, Qianyun; Ma, Jingyun; Xie, Qingmei; Zhang, Youngliang] South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. [Chang, Shuang] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Xue, Chunyi] Sun Yat Sen Univ, Coll Life Sci, State Key Lab Biocontrol, Guangzhou 510006, Guangdong, Peoples R China. RP Zhang, YL (reprint author), South China Agr Univ, Coll Anim Sci, Guangzhou 510642, Guangdong, Peoples R China. EM zhangyl@scau.edu.cn FU South China Agricultural University; Guangzhou work team project [2011A020102009] FX This work was supported by funds provided by South China Agricultural University and Guangzhou work team project (No 2011A020102009). NR 28 TC 10 Z9 14 U1 1 U2 22 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1674-9782 J9 J ANIM SCI BIOTECHNO JI J. Anim. Sci. Biotechnol. PD JUN 21 PY 2013 VL 4 AR 22 DI 10.1186/2049-1891-4-22 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD3XM UT WOS:000333179800001 PM 23786718 ER PT J AU Ingersoll, TE Sewall, BJ Amelon, SK AF Ingersoll, Thomas E. Sewall, Brent J. Amelon, Sybill K. TI Improved Analysis of Long-Term Monitoring Data Demonstrates Marked Regional Declines of Bat Populations in the Eastern United States SO PLOS ONE LA English DT Article ID WHITE-NOSE SYNDROME; GENERALIZED ADDITIVE-MODELS; BIG-EARED BAT; HIBERNATING BATS; MYOTIS-SODALIS; ECONOMIC IMPORTANCE; LINEAR-MODELS; FLYING FOXES; BROWN BATS; DISEASE AB Bats are diverse and ecologically important, but are also subject to a suite of severe threats. Evidence for localized bat mortality from these threats is well-documented in some cases, but long-term changes in regional populations of bats remain poorly understood. Bat hibernation surveys provide an opportunity to improve understanding, but analysis is complicated by bats' cryptic nature, non-conformity of count data to assumptions of traditional statistical methods, and observation heterogeneities such as variation in survey timing. We used generalized additive mixed models (GAMMs) to account for these complicating factors and to evaluate long-term, regional population trajectories of bats. We focused on four hibernating bat species - little brown myotis (Myotis lucifugus), tri-colored bat (Perimyotis subflavus), Indiana myotis (M. sodalis), and northern myotis (M. septentrionalis) - in a four-state region of the eastern United States during 1999-2011. Our results, from counts of nearly 1.2 million bats, suggest that cumulative declines in regional relative abundance by 2011 from peak levels were 71% (with 95% confidence interval of +/- 11%) in M. lucifugus, 34% (+/- 38%) in P. subflavus, 30% (+/- 26%) in M. sodalis, and 31% (+/- 18%) in M. septentrionalis. The M. lucifugus population fluctuated until 2004 before persistently declining, and the populations of the other three species declined persistently throughout the study period. Population trajectories suggest declines likely resulted from the combined effect of multiple threats, and indicate a need for enhanced conservation efforts. They provide strong support for a change in the IUCN Red List conservation status in M. lucifugus from Least Concern to Endangered within the study area, and are suggestive of a need to change the conservation status of the other species. Our modeling approach provided estimates of uncertainty, accommodated non-linearities, and controlled for observation heterogeneities, and thus has wide applicability for evaluating population trajectories in other wildlife species. C1 [Ingersoll, Thomas E.] Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. [Sewall, Brent J.] Temple Univ, Dept Biol, Philadelphia, PA 19122 USA. [Amelon, Sybill K.] Univ Missouri, No Res Stn, US Forest Serv, Columbia, MO USA. RP Ingersoll, TE (reprint author), Univ Tennessee, Natl Inst Math & Biol Synth, Knoxville, TN 37996 USA. EM tomingersoll@yahoo.com; bjsewall@temple.edu OI Amelon, Sybill/0000-0003-3940-8153 FU National Science Foundation; U.S. Department of Homeland Security; U.S. Department of Agriculture through NSF Award [EF-0832858]; University of Tennessee, Knoxville FX This work was conducted while TEI was a Postdoctoral Fellow at the National Institute for Mathematical and Biological Synthesis (http://www.nimbios.org), an Institute sponsored by the National Science Foundation (http://www.nsf.gov), the U.S. Department of Homeland Security (http://www.dhs.gov), and the U.S. Department of Agriculture (http://www.usda.gov) through NSF Award #EF-0832858, with additional support from The University of Tennessee, Knoxville (http://www.utk.edu). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 99 TC 11 Z9 12 U1 11 U2 129 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 21 PY 2013 VL 8 IS 6 AR e65907 DI 10.1371/journal.pone.0065907 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 170JP UT WOS:000320846500020 PM 23805192 ER PT J AU Peiffer, JA Flint-Garcia, SA De Leon, N McMullen, MD Kaeppler, SM Buckler, ES AF Peiffer, Jason A. Flint-Garcia, Sherry A. De Leon, Natalia McMullen, Michael D. Kaeppler, Shawn M. Buckler, Edward S. TI The Genetic Architecture of Maize Stalk Strength SO PLOS ONE LA English DT Article ID ASSOCIATION MAPPING POPULATION; RIND PENETROMETER RESISTANCE; GENOME-WIDE ASSOCIATION; ZEA-MAYS L.; QUANTITATIVE RESISTANCE; CRUSHING STRENGTH; COMPLEX TRAITS; LEAF-BLIGHT; SELECTION; MODEL AB Stalk strength is an important trait in maize (Zea mays L.). Strong stalks reduce lodging and maximize harvestable yield. Studies show rind penetrometer resistance (RPR), or the force required to pierce a stalk rind with a spike, is a valid approximation of strength. We measured RPR across 4,692 recombinant inbreds (RILs) comprising the maize nested association mapping (NAM) panel derived from crosses of diverse inbreds to the inbred, B73. An intermated B73xMo17 family (IBM) of 196 RILs and a panel of 2,453 diverse inbreds from the North Central Regional Plant Introduction Station (NCRPIS) were also evaluated. We measured RPR in three environments. Family-nested QTL were identified by joint-linkage mapping in the NAM panel. We also performed a genome-wide association study (GWAS) and genomic best linear unbiased prediction (GBLUP) in each panel. Broad sense heritability computed on a line means basis was low for RPR. Only 8 of 26 families had a heritability above 0.20. The NCRPIS diversity panel had a heritability of 0.54. Across NAM and IBM families, 18 family-nested QTL and 141 significant GWAS associations were identified for RPR. Numerous weak associations were also found in the NCRPIS diversity panel. However, few were linked to loci involved in phenylpropanoid and cellulose synthesis or vegetative phase transition. Using an identity-by-state (IBS) relationship matrix estimated from 1.6 million single nucleotide polymorphisms (SNPs) and RPR measures from 20% of the NAM panel, genomic prediction by GBLUP explained 64 +/- 2% of variation in the remaining RILs. In the NCRPIS diversity panel, an IBS matrix estimated from 681,257 SNPs and RPR measures from 20% of the panel explained 33 +/- 3% of variation in the remaining inbreds. These results indicate the high genetic complexity of stalk strength and the potential for genomic prediction to hasten its improvement. C1 [Peiffer, Jason A.; Buckler, Edward S.] Cornell Univ, Dept Genet & Plant Breeding, Ithaca, NY USA. [Flint-Garcia, Sherry A.; McMullen, Michael D.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO USA. [Flint-Garcia, Sherry A.; McMullen, Michael D.] Univ Missouri, Div Plant Sci, Columbia, MO USA. [De Leon, Natalia; Kaeppler, Shawn M.] Univ Wisconsin, Dept Agron, Madison, WI USA. [Buckler, Edward S.] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Peiffer, JA (reprint author), N Carolina State Univ, Bioinformat Res Ctr, Dept Genet, Raleigh, NC 27695 USA. EM japeiffe@ncsu.edu OI Buckler, Edward/0000-0002-3100-371X; Kaeppler, Shawn/0000-0002-5964-1668 FU National Science Foundation [0820619]; United States Department of Agriculture - Agricultural Research Service FX This work was supported in part by National Science Foundation award 0820619 and the United States Department of Agriculture - Agricultural Research Service. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 26 Z9 27 U1 4 U2 55 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 20 PY 2013 VL 8 IS 6 AR e67066 DI 10.1371/journal.pone.0067066 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 190LY UT WOS:000322342800117 PM 23840585 ER PT J AU Roh, MS AF Roh, Mark S. TI Growth and flowering response of 'Monte Grace' lily grown in media amended with bioplastic pellets and in bioplastic pots containing feather fibers SO SCIENTIA HORTICULTURAE LA English DT Article DE Flower bud blast; Foliar analysis; Growing medium analysis; Hybrid lily; Optimum nutrient concentration; Slow release fertilizer ID CONTROLLED-RELEASE FERTILIZERS; LONGIFLORUM NELLIE WHITE; BIOBASED PLASTIC RESINS; NEUTRAL-PH SUBSTRATE; NITROGEN-FERTILIZER; OUTDOOR ENVIRONMENT; NUTRIENT RELEASE; ORGANIC SOURCES; TEMPERATURE; BEGONIA AB This study was conducted to evaluate the growth and flowering of Lilium longiflorum Thunb. x L. x elegans Thunb. (LA) hybrid 'Monte Grace' when grown in media amended with three bioplastic resin pellets (Pel) containing chicken feather fibers (Pel 29, Pel 32, and Pel 45) and in injection molded bioplastic (BP) pots processed with three pellets. Bulbs were planted in a growing medium amended (GMA) with 4 or 8g of each pellet or planted in the BP pots placed in the center of plastic pots. After potting, 2g of slow release fertilizer (SRF) were applied to the surface of the growing medium. The changes in color of BP 29 and BP 32 pots suggested that these two BP pots may biodegrade faster than BP 45 pots. The growth and development of 'Monte Grace' in growing medium amended with Pel 32 and also in BP 32 pots were significantly reduced, resulting in flower bud blast. The negative effects of Pet 32 in the growing medium and in BP 32 pots were not reduced by addition of SRF. The growing medium pH was lowered significantly by the addition of SRF regardless of the BP pot types. However, growth and development was not affected by low pH. Concentrations of macro- and micro-elements fell into suggested and optimum ranges as reported for Lilium species and hybrids; however, the calcium concentration was lower than the optimum concentration, although, no nutrient deficiency symptoms were observed. Although extra nitrogen becomes available from Pel 32 or BP 32 pots, it is not recommended for lily production. The use of Pel 45 or BP 45 pots with SRF proved to be the most effective bioplastic material when plant growth, growing medium, and foliar analysis were evaluated. Published by Elsevier B.V. C1 ARS, USDA, USNA, Floral & Nursery Plants Res Unit, Beltsville, MD 20705 USA. RP Roh, MS (reprint author), ARS, USDA, USNA, Floral & Nursery Plants Res Unit, B-010A,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM marksroh@gmail.com NR 22 TC 1 Z9 2 U1 1 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4238 J9 SCI HORTIC-AMSTERDAM JI Sci. Hortic. PD JUN 20 PY 2013 VL 157 BP 52 EP 59 DI 10.1016/j.scienta.2013.04.012 PG 8 WC Horticulture SC Agriculture GA 168AY UT WOS:000320679300008 ER PT J AU Saathoff, AJ Donze, T Palmer, NA Bradshaw, J Heng-Moss, T Twigg, P Tobias, CM Lagrimini, M Sarath, G AF Saathoff, Aaron J. Donze, Teresa Palmer, Nathan A. Bradshaw, Jeff Heng-Moss, Tiffany Twigg, Paul Tobias, Christian M. Lagrimini, Mark Sarath, Gautam TI Towards uncovering the roles of switchgrass peroxidases in plant processes SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE switchgrass; peroxidases; lignin; Hemiptera; biotic stress; ROS ID PANICUM-VIRGATUM L.; EXPRESSED SEQUENCE TAGS; MISCANTHUS X GIGANTEUS; CLASS-III PEROXIDASES; LIGNIN BIOSYNTHESIS; ARABIDOPSIS-THALIANA; DEFENSE RESPONSES; GENE-EXPRESSION; BIOMASS YIELD; APOPLASTIC PEROXIDASE AB Herbaceous perennial plants selected as potential biofuel feedstocks had been understudied at the genomic and functional genomic levels. Recent investments, primarily by the U.S. Department of Energy, have led to the development of a number of molecular resources for bioenergy grasses, such as the partially annotated genome for switchgrass (Panicum virgatum L.), and some related diploid species. In its current version, the switchgrass genome contains 65,878 gene models arising from the A and B genomes of this tetraploid grass. The availability of these gene sequences provides a framework to exploit transcriptomic data obtained from next-generation sequencing platforms to address questions of biological importance. One such question pertains to discovery of genes and proteins important for biotic and abiotic stress responses, and how these components might affect biomass quality and stress response in plants engineered for a specific end purpose. It can be expected that production of switchgrass on marginal lands will expose plants to diverse stresses, including herbivory by insects. Class III plant peroxidases have been implicated in many developmental responses such as lignification and in the adaptive responses of plants to insect feeding. Here, we have analyzed the class III peroxidases encoded by the switchgrass genome, and have mined available transcriptomic datasets to develop a first understanding of the expression profiles of the class III peroxidases in different plant tissues. Lastly, we have identified switchgrass peroxidases that appear to be orthologs of enzymes shown to play key roles in lignification and plant defense responses to hemipterans. C1 [Saathoff, Aaron J.; Palmer, Nathan A.; Sarath, Gautam] Univ Nebraska, ARS, Grain Forage & Bioenergy Res Unit, USDA, Lincoln, NE 68583 USA. [Saathoff, Aaron J.; Palmer, Nathan A.; Lagrimini, Mark; Sarath, Gautam] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Donze, Teresa; Bradshaw, Jeff; Heng-Moss, Tiffany] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Twigg, Paul] Univ Nebraska Kearney, Dept Biol, Kearney, NE USA. [Tobias, Christian M.] ARS, Genom & Gene Discovery Res Unit, USDA, Albany, CA USA. RP Saathoff, AJ (reprint author), Univ Nebraska, ARS, Grain Forage & Bioenergy Res Unit, USDA, 137 Keim Hall, Lincoln, NE 68583 USA. EM aaron.saathoff@ars.usda.gov RI Tobias, Christian/B-6602-2009 OI Tobias, Christian/0000-0002-7881-750X FU USDA-NIFA [2011-67009-30096]; Office of Science (BER); U.S. Department of Energy [DE-AI02-09ER64829]; USDA-ARS CRIS [5440-21000-030-00D] FX This work was supported in part by USDA-NIFA Grant Number 2011-67009-30096, The Office of Science (BER), U.S. Department of Energy Grant Number DE-AI02-09ER64829, and by the USDA-ARS CRIS project 5440-21000-030-00D. The U.S. Department of Agriculture, Agricultural Research Service, is an equal opportunity/affirmative action employer and all agency services are available without discrimination. Mention of commercial products and organizations in this manuscript is solely to provide specific information. It does not constitute endorsement by USDA-ARS over other products and organizations not mentioned. All sequences used in this work are publically available through the NCBI and are shown in Table A1 in Appendix. The PviDraft sequence data used in this study were produced by the U.S. Department of Energy Joint Genome Institute. NR 140 TC 12 Z9 13 U1 6 U2 32 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 19 PY 2013 VL 4 AR 202 DI 10.3389/fpls.2013.00202 PG 12 WC Plant Sciences SC Plant Sciences GA 296ED UT WOS:000330167000001 PM 23802005 ER PT J AU Nwugo, CC Duan, YP Lin, H AF Nwugo, Chika C. Duan, Yongping Lin, Hong TI Study on Citrus Response to Huanglongbing Highlights a Down-Regulation of Defense-Related Proteins in Lemon Plants Upon 'Ca. Liberibacter asiaticus' Infection SO PLOS ONE LA English DT Article ID SWEET ORANGE; GENE-EXPRESSION; BIOTIC STRESS; SINENSIS; DISEASE; ARABIDOPSIS; RESISTANCE; DEFICIENCY; DIFFERENTIATION; IDENTIFICATION AB Citrus huanglongbing (HLB) is a highly destructive disease of citrus presumably caused by 'Candidatus Liberibacter asiaticus' (Las), a gram-negative, insect-transmitted, phloem-limited alpha-proteobacterium. Although almost all citrus plants are susceptible to HLB, reports have shown reduced susceptibility to Las infection in lemon (Citrus limon) plants. The aim of this study is to identify intra-species specific molecular mechanisms associated with Las-induced responses in lemon plants. To achieve this, comparative 2-DE and mass spectrometry, in addition to Inductively Coupled Plasma Spectroscopy (ICPS) analyses, were applied to investigate differences in protein accumulation and the concentrations of cationic elements in leaves of healthy and Las-infected lemon plants. Results showed a differential accumulation of 27 proteins, including an increase in accumulation of starch synthase but decrease in the production of photosynthesis-related proteins in Las-infected lemon plants compared to healthy plants. Furthermore, there was a 6% increase (P > 0.05) in K concentration in leaves of lemon plants upon Las infection, which support results from previous studies and might represent a common response pattern of citrus plants to Las infection. Interestingly, contrary to reports from prior studies, this study showed a general reduction in the production of defense-related pathogen-response proteins but a 128% increase in Zn concentration in lemon plants in response to Las infection. Taken together, this study sheds light on general and intra-species specific responses associated with the response of citrus plants to Las. C1 [Nwugo, Chika C.; Lin, Hong] ARS, San Joaquin Valley Agr Sci Ctr, Dept Agr, Parlier, CA USA. [Duan, Yongping] ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. RP Lin, H (reprint author), ARS, San Joaquin Valley Agr Sci Ctr, Dept Agr, Parlier, CA USA. EM hong.lin@ars.usda.gov FU US Department of Agriculture, Agricultural Research Service FX Funding for this project was provided by the US Department of Agriculture, Agricultural Research Service. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 56 TC 14 Z9 16 U1 2 U2 33 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 19 PY 2013 VL 8 IS 6 AR e67442 DI 10.1371/journal.pone.0067442 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 190ST UT WOS:000322361200135 PM 23922636 ER PT J AU Kaufman, RC Wilson, JD Bean, SR Presley, DR Blanco-Canqui, H Mikha, M AF Kaufman, R. C. Wilson, J. D. Bean, S. R. Presley, D. R. Blanco-Canqui, H. Mikha, M. TI Effect of Nitrogen Fertilization and Cover Cropping Systems on Sorghum Grain Characteristics SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE sorghum; nitrogen fertilization; cover crop; protein; hardness ID WINTER-WHEAT; QUALITY; CROPS; YIELD; ROTATION AB Cover crop treatments and nitrogen (N) fertilization rates were investigated for their impact on sorghum grain quality attributes. Sorghum was planted in field plots treated with differing cover cropping systems and fertilization rates. The size (weight and diameter) and hardness of the kernels were influenced by both the cover crop and N rates. The protein content increased as the N rate increased and also with the addition of cover crops to the system. The protein digestibility values and starch granule size distributions were not affected by N rate or the cover cropping treatments. Soil properties were tested to determine relationships with grain quality attributes. The utilization of cover crops appears to increase the protein content without causing a deleterious effect on protein digestibility. The end-product quality is not hampered by the use of beneficial cropping systems necessary for sustainable agriculture. C1 [Kaufman, R. C.; Wilson, J. D.; Bean, S. R.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Presley, D. R.] Kansas State Univ, Ctr Sci, Dept Agron, Manhattan, KS 66506 USA. [Blanco-Canqui, H.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE 68583 USA. [Mikha, M.] USDA ARS, Cent Great Plains Res Stn, Akron, CO 80720 USA. RP Wilson, JD (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM jeff.d.wilson@ars.usda.gov OI Bean, Scott/0000-0001-8678-8094 NR 20 TC 4 Z9 4 U1 3 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 19 PY 2013 VL 61 IS 24 BP 5715 EP 5719 DI 10.1021/jf401179n PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 171BA UT WOS:000320898600013 PM 23705643 ER PT J AU Huang, HQ Xie, ZH Boue, SM Bhatnagar, D Yokoyama, W Yu, LL Wang, TTY AF Huang, Haiqiu Xie, Zhuohong Boue, Stephen M. Bhatnagar, Deepak Yokoyama, Wallace Yu, Liangli (Lucy) Wang, Thomas T. Y. TI Cholesterol-Lowering Activity of Soy-Derived Glyceollins in the Golden Syrian Hamster Model SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE cholesterol; glyceollins; hamster ID ASPERGILLUS-SOJAE; DIETARY-CHOLESTEROL; CARDIOVASCULAR RISK; PPAR-ALPHA; RECEPTOR; SOYBEANS; PROTEIN; ATHEROSCLEROSIS; PHYTOALEXINS; METABOLISM AB Hypercholesterolemia is one of the major factors contributing to the risk of cardiovascular disease (CVD), which is the leading cause of death in developed countries. Consumption of soy foods has been recognized to lower the risk of CVD, and phytochemicals in soy are believed to contribute to the health benefits. Glyceollin is one of the candidate phytochemicals synthesized in stressed soy that may account for many unique biological activities. In this study, the in vivo cholesterol-lowering effect of glyceollins was investigated. Male golden Syrian hamsters were fed diets including (1) 36 kcal% fat diet, (2) 36 kcal% fat diet containing 250 mg/kg diet glyceollins, or (3) chow for 28 days. Hepatic cholesterol esters and free cholesterol, hepatic total lipid content, plasma lipoproteins, fecal bile acid, fecal total cholesterol, and cholesterol metabolism related gene expressions were measured. Glyceollin supplementation led to significant reduction of plasma VLDL, hepatic cholesterol esters, and total lipid content. Consistent with changes in circulating cholesterol, glyceollin supplementation also altered expression of the genes related to cholesterol metabolism in the liver. In contrast, no change in plasma LDL and HDL, fecal bile acid, or cholesterol content was observed. The cholesterol-lowering effect of glyceollins appeared not to go through the increase of bile excretion. These results supported glyceollins' role as novel soy-derived cholesterol-lowering phytochemicals that may contribute to soy's health effects. C1 [Huang, Haiqiu; Xie, Zhuohong; Yu, Liangli (Lucy)] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Boue, Stephen M.] ARS, USDA, New Orleans, LA 70179 USA. [Bhatnagar, Deepak] ARS, USDA, New Orleans, LA 70124 USA. [Yokoyama, Wallace] ARS, USDA, Albany, CA 94710 USA. [Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, USDA, Beltsville, MD 20705 USA. RP Yu, LL (reprint author), Univ Maryland, Dept Nutr & Food Sci, 0112 Skinner Bldg, College Pk, MD 20742 USA. EM lyu5@umd.edu; Tom.Wang@ars.usda.gov RI Xie, Zhuohong/P-3599-2014 OI Xie, Zhuohong/0000-0001-8009-3523 FU USDA [1235-51530-053-00D] FX This research was supported by USDA appropriate fund Project 1235-51530-053-00D (T.T.Y.W.). NR 58 TC 7 Z9 7 U1 0 U2 15 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 19 PY 2013 VL 61 IS 24 BP 5772 EP 5782 DI 10.1021/jf400557p PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 171BA UT WOS:000320898600020 PM 23697397 ER PT J AU Wang, WJ He, HS Spetich, MA Shifley, SR Thompson, FR Fraser, JS AF Wang, Wen J. He, Hong S. Spetich, Martin A. Shifley, Stephen R. Thompson, Frank R., III Fraser, Jacob S. TI Modeling the Effects of Harvest Alternatives on Mitigating Oak Decline in a Central Hardwood Forest Landscape SO PLOS ONE LA English DT Article ID SPECIES COMPOSITION; NORTHERN ARKANSAS; OZARK HIGHLANDS; MORTALITY; MISSOURI; GROWTH; INDEX AB Oak decline is a process induced by complex interactions of predisposing factors, inciting factors, and contributing factors operating at tree, stand, and landscape scales. It has greatly altered species composition and stand structure in affected areas. Thinning, clearcutting, and group selection are widely adopted harvest alternatives for reducing forest vulnerability to oak decline by removing susceptible species and declining trees. However, the long-term, landscape-scale effects of these different harvest alternatives are not well studied because of the limited availability of experimental data. In this study, we applied a forest landscape model in combination with field studies to evaluate the effects of the three harvest alternatives on mitigating oak decline in a Central Hardwood Forest landscape. Results showed that the potential oak decline in high risk sites decreased strongly in the next five decades irrespective of harvest alternatives. This is because oak decline is a natural process and forest succession (e.g., high tree mortality resulting from intense competition) would eventually lead to the decrease in oak decline in this area. However, forest harvesting did play a role in mitigating oak decline and the effectiveness varied among the three harvest alternatives. The group selection and clearcutting alternatives were most effective in mitigating oak decline in the short and medium terms, respectively. The long-term effects of the three harvest alternatives on mitigating oak decline became less discernible as the role of succession increased. The thinning alternative had the highest biomass retention over time, followed by the group selection and clearcutting alternatives. The group selection alternative that balanced treatment effects and retaining biomass was the most viable alternative for managing oak decline. Insights from this study may be useful in developing effective and informed forest harvesting plans for managing oak decline. C1 [Wang, Wen J.; He, Hong S.; Fraser, Jacob S.] Univ Missouri, Sch Nat Resources, Columbia, MO 65211 USA. [Spetich, Martin A.] US Forest Serv, USDA, Arkansas Forestry Sci Lab, Southern Res Stn, Hot Spring, AR USA. [Shifley, Stephen R.; Thompson, Frank R., III] US Forest Serv, USDA, No Res Stn, Columbia, MO USA. RP He, HS (reprint author), Univ Missouri, Sch Nat Resources, Columbia, MO 65211 USA. EM heh@missouri.edu OI He, Hong S./0000-0002-3983-2512 FU U.S Forest Service South Central Research Station; University of Missouri GIS Mission Enhancement Program FX This research was funded by the U.S Forest Service South Central Research Station, and the University of Missouri GIS Mission Enhancement Program. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 40 TC 6 Z9 6 U1 1 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 18 PY 2013 VL 8 IS 6 AR e66713 DI 10.1371/journal.pone.0066713 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 166SA UT WOS:000320576400153 PM 23825560 ER PT J AU Anderson, TK Sukhdeo, MVK AF Anderson, Tavis K. Sukhdeo, Michael V. K. TI Qualitative community stability determines parasite establishment and richness in estuarine marshes SO PEERJ LA English DT Article DE Food web; Complex life cycle; Parasite establishment; Qualitative stability; Parasite ecology; Parasite diversity; Biodiversity; Ecological network AB The establishment of parasites with complex life cycles is generally thought to be regulated by free-living species richness and the stability of local ecological interactions. In this study, we test the prediction that stable host communities are prerequisite for the establishment of complex multi-host parasite life cycles. The colonization of naive killifish, Fundulus heteroclitus, by parasites was investigated in 4 salt marsh sites that differed in time since major ecological restoration, and which provided a gradient in free-living species richness. The richness of the parasite community, and the rate at which parasite species accumulated in the killifish, were similar between the low diversity unrestored site and the two high diversity (10- and 20-year) restored marsh sites. The parasite community in the newly restored marsh (0 year) included only directly-transmitted parasite species. To explain the paradox of a low diversity, highly invaded salt marsh (unrestored) having the same parasite community as highly diverse restored marsh sites (10 and 20 yrs) we assessed qualitative community stability. We find a significant correlation between system stability and parasite species richness. These data suggest a role for local stability in parasite community assembly, and support the idea that stable trophic relationships are required for the persistence of complex parasite life cycles. C1 [Anderson, Tavis K.] Rutgers State Univ, Grad Program Ecol & Evolut, New Brunswick, NJ 08903 USA. [Anderson, Tavis K.] USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA USA. [Sukhdeo, Michael V. K.] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08903 USA. RP Anderson, TK (reprint author), Rutgers State Univ, Grad Program Ecol & Evolut, New Brunswick, NJ 08903 USA. EM tavis.anderson@ars.usda.gov OI Anderson, Tavis/0000-0002-3138-5535 FU Willis A. Reid Jr. student research grant (The American Society of Parasitologists); Rutgers University Foundation Parasite Research Fund FX This research was funded in part by a Willis A. Reid Jr. student research grant (The American Society of Parasitologists) to TKA and by the Rutgers University Foundation Parasite Research Fund to TKA and MVKS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 1 Z9 1 U1 2 U2 8 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD JUN 18 PY 2013 VL 1 AR UNSP e92 DI 10.7717/peerj.92 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA V36BA UT WOS:000209187300002 PM 23802092 ER PT J AU Gilbert, MK Turley, RB Kim, HJ Li, P Thyssen, G Tang, YH Delhom, CD Naoumkina, M Fang, DD AF Gilbert, Matthew K. Turley, Rickie B. Kim, Hee Jin Li, Ping Thyssen, Gregory Tang, Yuhong Delhom, Christopher D. Naoumkina, Marina Fang, David D. TI Transcript profiling by microarray and marker analysis of the short cotton (Gossypium hirsutum L.) fiber mutant Ligon lintless-1 (Li-1) SO BMC GENOMICS LA English DT Article DE Cotton; Ligon Lintless-1; Microarray; Cotton fiber elongation; Microsatellite markers ID CELLULOSE SYNTHASE COMPLEXES; SIMPLE SEQUENCE REPEAT; PLANT-CELL ELONGATION; GENE-EXPRESSION; UPLAND COTTON; ARABIDOPSIS-THALIANA; FUNCTIONAL GENOMICS; SEED-GERMINATION; WALL SYNTHESIS; GROWTH AB Background: Cotton fiber length is very important to the quality of textiles. Understanding the genetics and physiology of cotton fiber elongation can provide valuable tools to the cotton industry by targeting genes or other molecules responsible for fiber elongation. Ligon Lintless-1 (Li-1) is a monogenic mutant in Upland cotton (Gossypium hirsutum) which exhibits an early cessation of fiber elongation resulting in very short fibers (< 6 mm) at maturity. This presents an excellent model system for studying the underlying molecular and cellular processes involved with cotton fiber elongation. Previous reports have characterized Li-1 at early cell wall elongation and during later secondary cell wall synthesis, however there has been very limited analysis of the transition period between these developmental time points. Results: Physical and morphological measurements of the Li-1 mutant fibers were conducted, including measurement of the cellulose content during development. Affymetrix microarrays were used to analyze transcript profiles at the critical developmental time points of 3 days post anthesis (DPA), the late elongation stage of 12 DPA and the early secondary cell wall synthesis stage of 16 DPA. The results indicated severe disruption to key hormonal and other pathways related to fiber development, especially pertaining to the transition stage from elongation to secondary cell wall synthesis. Gene Ontology enrichment analysis identified several key pathways at the transition stage that exhibited altered regulation. Genes involved in ethylene biosynthesis and primary cell wall rearrangement were affected, and a primary cell wall-related cellulose synthase was transcriptionally repressed. Linkage mapping using a population of 2,553 F-2 individuals identified SSR markers associated with the Li-1 genetic locus on chromosome 22. Linkage mapping in combination with utilizing the diploid G. raimondii genome sequences permitted additional analysis of the region containing the Li-1 gene. Conclusions: The early termination of fiber elongation in the Li-1 mutant is likely controlled by an early upstream regulatory factor resulting in the altered regulation of hundreds of downstream genes. Several elongation-related genes that exhibited altered expression profiles in the Li-1 mutant were identified. Molecular markers closely associated with the Li-1 locus were developed. Results presented here will lay the foundation for further investigation of the genetic and molecular mechanisms of fiber elongation. C1 [Gilbert, Matthew K.; Kim, Hee Jin; Li, Ping; Thyssen, Gregory; Naoumkina, Marina; Fang, David D.] USDA ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. [Turley, Rickie B.] USDA ARS, Crop Genet Res Unit, Mid South Area, Stoneville, MS 38772 USA. [Tang, Yuhong] Samuel Roberts Noble Fdn Inc, Genom Core Facil, Ardmore, OK 73401 USA. [Delhom, Christopher D.] USDA ARS, Cotton Struct & Qual Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Fang, DD (reprint author), USDA ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459 FU United States Department of Agriculture-Agricultural Research Service CRIS project [6435-21000-016-00D] FX This research was funded by United States Department of Agriculture-Agricultural Research Service CRIS project 6435-21000-016-00D. We greatly thank Mr. Chris Florane and Mrs. Tracy Condon for their assistance with field work, sample collection, and fiber structure analysis. We thank Mrs. Holly King for her expertise in fiber sectioning and imaging. Our great appreciation goes to Mrs. Sheron Simpson and Dr. Brian Scheffler at Genomics and Bioinformatics Research Unit at Stoneville, MS for their excellent support in SSR marker analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture that is an equal opportunity provider and employer. NR 83 TC 16 Z9 17 U1 2 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 17 PY 2013 VL 14 AR 403 DI 10.1186/1471-2164-14-403 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 177PT UT WOS:000321388200001 PM 23767687 ER PT J AU Lopes, AP Sousa, S Dubey, JP Ribeiro, AJ Silvestre, R Cotovio, M Schallig, HDFH Cardoso, L Cordeiro-da-Silva, A AF Lopes, Ana Patricia Sousa, Susana Dubey, J. P. Ribeiro, Ana J. Silvestre, Ricardo Cotovio, Mario Schallig, Henk D. F. H. Cardoso, Luis Cordeiro-da-Silva, Anabela TI Prevalence of antibodies to Leishmania infantum and Toxoplasma gondii in horses from the north of Portugal SO PARASITES & VECTORS LA English DT Article DE Antibodies; Horses; Leishmania infantum; Portugal; Toxoplasma gondii ID INFECTION; SEROPREVALENCE; SPAIN; DOGS; HEALTHY AB Background: Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. Findings: Antibodies to L. infantum were detected by the direct agglutination test (DAT); seven (4.0%) horses were seropositive with DAT titres of 200 (n = 5), 800 (n = 1) and >= 1600 (n = 1). Antibodies to T. gondii were assayed by the modified agglutination test (MAT); 23 (13.3%) horses were seropositive with MAT titres of 20 (n = 13), 40 (n = 5), 80 (n = 3) and >= 160 (n = 2). No statistical differences were found among equine categories of gender (female, male and gelding), age (1.5-6, 7-12 and 13-30 years), type of housing (indoors and mixed/outdoors), ability (recreation, farming and sports) and clinical status (apparently healthy and sick) for both agents. Conclusions: Horses are exposed to and may be infected with L. infantum and T. gondii in the north of Portugal. C1 [Lopes, Ana Patricia; Cotovio, Mario; Cardoso, Luis] Univ Tras Os Montes & Alto Douro UTAD, Sch Agr & Vet Sci, Dept Vet Sci, Vila Real, Portugal. [Lopes, Ana Patricia; Cotovio, Mario] UTAD, Anim & Vet Res Ctr, Vila Real, Portugal. [Sousa, Susana; Silvestre, Ricardo; Cardoso, Luis; Cordeiro-da-Silva, Anabela] Univ Porto, IBMC, Parasite Dis Grp, P-4100 Oporto, Portugal. [Dubey, J. P.] Agr Res Serv, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. [Ribeiro, Ana J.] UTAD, Vet Hosp, Vila Real, Portugal. [Schallig, Henk D. F. H.] Royal Trop Inst, KIT, Dept Parasitol, NL-1105 AZ Amsterdam, Netherlands. [Cordeiro-da-Silva, Anabela] UP, Fac Farm, Dept Ciencias Biol, Oporto, Portugal. RP Cardoso, L (reprint author), Univ Tras Os Montes & Alto Douro UTAD, Sch Agr & Vet Sci, Dept Vet Sci, Vila Real, Portugal. EM lcardoso@utad.pt RI Cardoso, Luis/J-8310-2013; Cordeiro-da-Silva, Anabela/J-7807-2013; Silvestre, Ricardo/C-7243-2011; sousa, susana/D-8567-2013; Lopes, Ana Patricia/K-4671-2013; OI Cardoso, Luis/0000-0002-6145-7560; Cordeiro-da-Silva, Anabela/0000-0003-2392-6087; Silvestre, Ricardo/0000-0002-9270-2717; sousa, susana/0000-0003-3891-3924; Ribeiro, Ana Jacinta/0000-0002-6929-8138; Lopes, Ana Patricia/0000-0002-8182-5674 FU FEDER funds through the Operational Competitiveness Programme - COMPETE; National funds through FCT - Fundacao para a Ciencia e a Tecnologia [FCOMP-01-0124-FEDER-014658, PTDC/CVT/110732/2009]; Programa Ciencia by Programa Operacional Potencial Humano - Quadro de Referencia Estrategica Nacional - Tipologia 4.2 Promocao do Emprego Cientifico; Fundo Social Europeu; national funding from the Ministry of Science, Technology and Higher Education FX The authors would like to thank Dr. Jose M. Maia for collecting and characterizing samples from horses. This work was funded by FEDER funds through the Operational Competitiveness Programme - COMPETE and by National funds through FCT - Fundacao para a Ciencia e a Tecnologia under the project FCOMP-01-0124-FEDER-014658 (PTDC/CVT/110732/2009). RS was supported by Programa Ciencia, financed by Programa Operacional Potencial Humano - Quadro de Referencia Estrategica Nacional - Tipologia 4.2 Promocao do Emprego Cientifico, cofunded by Fundo Social Europeu and national funding from the Ministry of Science, Technology and Higher Education. NR 21 TC 9 Z9 9 U1 0 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD JUN 17 PY 2013 VL 6 AR 178 DI 10.1186/1756-3305-6-178 PG 4 WC Parasitology SC Parasitology GA 167YY UT WOS:000320672700002 PM 23773870 ER PT J AU Burner, DM Pote, DH Mackown, CT AF Burner, D. M. Pote, D. H. Mackown, C. T. TI Short-Term Soil Responses for an Emulated Loblolly Pine Silvopasture SO COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS LA English DT Article DE Alley crop; Pinus taeda L; plantation design; silvopasture; soil nutrients ID CARBON-DIOXIDE EMISSIONS; CROPPING SYSTEM; ORGANIC-MATTER; POULTRY LITTER; SODA-LIME; NITROGEN; PHOSPHORUS; WATER; MINERALIZATION; FERTILIZATION AB Pine (Pinus spp. L.) stands are often overstocked early in the tree rotation, prior to initial thinning. While pre- and/or post-thinning fertilizer applications are best-management practices to optimize growth of southern pines, there can be poor nitrogen (N) utilization and adverse environmental impacts associated with fertilization. Our objective was to determine short-term (3-year) soil responses of an emulated loblolly pine (P. taeda L.) silvopasture, which received a single application of commercial N-phosphorus (P)-potassium (K) fertilizer (CF) or pelletized poultry litter (PPL) applied at about midrotation (12 years postplanting). Compared to the control, CF decreased soil pH at depths of 0-10 and 10-30 cm, and PPL increased Mehlich 3 available P at 0-10 cm. Fertilizer responses were found for soil extractable ammonium (NH4 (+))-N, and nitrate (NO3 (-))-N concentrations, mineral N ha(-1), pH, and available P but not for diel carbon dioxide-carbon (CO2-C) flux, total C, and total N. Total soil C, total soil N, pH, and available P decreased with depth, whereas mineral N ha(-1) and 1 M potassium chloride (KCl)-extractable aluminum (Al) increased with depth. These results further our understanding of the nutrient dynamics during alley cropping of an upland soil and demonstrate the challenge in detecting short-term responses with fertilization. C1 [Burner, D. M.; Pote, D. H.] ARS, USDA, DBSFRC, Booneville, AR 72927 USA. [Mackown, C. T.] ARS, USDA, GRL, El Reno, OK USA. RP Burner, DM (reprint author), ARS, USDA, DBSFRC, 6883 South State Highway 23, Booneville, AR 72927 USA. EM David.Burner@ars.usda.gov NR 42 TC 0 Z9 0 U1 1 U2 6 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 0010-3624 J9 COMMUN SOIL SCI PLAN JI Commun. Soil Sci. Plant Anal. PD JUN 17 PY 2013 VL 44 IS 11 BP 1708 EP 1721 DI 10.1080/00103624.2013.769561 PG 14 WC Agronomy; Plant Sciences; Chemistry, Analytical; Soil Science SC Agriculture; Plant Sciences; Chemistry GA 162XU UT WOS:000320300100005 ER PT J AU Singh, SK Badgujar, G Reddy, VR Fleisher, DH Bunce, JA AF Singh, Shardendu K. Badgujar, Girish Reddy, Vangimalla R. Fleisher, David H. Bunce, James A. TI Carbon dioxide diffusion across stomata and mesophyll and photo-biochemical processes as affected by growth CO2 and phosphorus nutrition in cotton SO JOURNAL OF PLANT PHYSIOLOGY LA English DT Article DE Acclimation; Carboxylation; Down regulation; Fluorescence; Quantum yield ID PHOTOSYNTHETIC QUANTUM YIELD; ELEVATED CO2; ATMOSPHERIC CO2; GOSSYPIUM-HIRSUTUM; SPINACH LEAVES; GAS-EXCHANGE; CONDUCTANCE; ACCLIMATION; NITROGEN; PLANTS AB Nutrients such as phosphorus may exert a major control over plant response to rising atmospheric carbon dioxide concentration (CO2), which is projected to double by the end of the 21st century. Elevated CO2 overcome the diffusional limitations to photosynthesis posed by stomata and mesophyll and alter the photo-biochemical limitations resulting from phosphorus deficiency. To evaluate these ideas, cotton (Gossypium hirsutum) was grown in controlled environment growth chambers with three levels of phosphate (Pi) supply (0.2, 0.05 and 0.01 mM) and two levels of CO2 concentration (ambient 400 and elevated 800 mu mol mol(-1)) under optimum temperature and irrigation. Phosphate deficiency drastically inhibited photosynthetic characteristics and decreased cotton growth for both CO2 treatments. Under Pi stress, an apparent limitation to the photosynthetic potential was evident by CO2 diffusion through stomata and mesophyll, impairment of photosystem functioning and inhibition of biochemical process including the carboxylation efficiency of ribulose-1,5-bisphosphate carboxylase/oxyganase and the rate of ribulose-1,5-bisphosphate regeneration. The diffusional limitation posed by mesophyll was up to 58% greater than the limitation due to stomatal conductance (g(s)) under Pi stress. As expected, elevated CO2 reduced these diffusional limitations to photosynthesis across Pi levels; however, it failed to reduce the photo-biochemical limitations to photosynthesis in phosphorus deficient plants. Acclimation/down regulation of photosynthetic capacity was evident under elevated CO2 across Pi treatments. Despite a decrease in phosphorus, nitrogen and chlorophyll concentrations in leaf tissue and reduced stomatal conductance at elevated CO2, the rate of photosynthesis per unit leaf area when measured at the growth CO2 concentration tended to be higher for all except the lowest Pi treatment. Nevertheless, plant biomass increased at elevated CO2 across Pi nutrition with taller plants, increased leaf number and larger leaf area. (c) 2013 Elsevier GmbH. All rights reserved. C1 [Singh, Shardendu K.] Univ Maryland, Wye Res & Educ Ctr, College Pk, MD 20742 USA. [Singh, Shardendu K.; Badgujar, Girish; Reddy, Vangimalla R.; Fleisher, David H.; Bunce, James A.] ARS, Crop Syst & Global Change Lab, USDA, Beltsville, MD 20705 USA. [Badgujar, Girish] Asian Inst Technol, Pathum Thani, Thailand. RP Singh, SK (reprint author), ARS, Crop Syst & Global Change Lab, USDA, BARC West, Bldg 001,Rm 342,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM shardendu.singh@ars.usda.gov NR 51 TC 14 Z9 14 U1 4 U2 74 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0176-1617 J9 J PLANT PHYSIOL JI J. Plant Physiol. PD JUN 15 PY 2013 VL 170 IS 9 BP 801 EP 813 DI 10.1016/j.jplph.2013.01.001 PG 13 WC Plant Sciences SC Plant Sciences GA 168BS UT WOS:000320681300002 PM 23384758 ER PT J AU Miki, Y Pogni, R Acebes, S Lucas, F Fernandez-Fueyo, E Baratto, MC Fernandez, MI de los Rios, V Ruiz-Duenas, FJ Sinicropi, A Basosi, R Hammel, KE Guallar, V Martinez, AT AF Miki, Yuta Pogni, Rebecca Acebes, Sandra Lucas, Fatima Fernandez-Fueyo, Elena Baratto, Maria Camilla Fernandez, Maria I. de los Rios, Vivian Ruiz-Duenas, Francisco J. Sinicropi, Adalgisa Basosi, Riccardo Hammel, Kenneth E. Guallar, Victor Martinez, Angel T. TI Formation of a tyrosine adduct involved in lignin degradation by Trametopsis cervina lignin peroxidase: a novel peroxidase activation mechanism SO BIOCHEMICAL JOURNAL LA English DT Article DE EPR; lignin model compound; lignin peroxidase (LiP); molecular docking; MS; quantum mechanics/molecular mechanics (QM/MM); tyrosine adduct ID SITE-DIRECTED MUTAGENESIS; RANGE ELECTRON-TRANSFER; VERATRYL ALCOHOL; VERSATILE PEROXIDASE; TRAMETES-CERVINA; OXIDATION; INSIGHTS; ENZYMES AB LiP (lignin peroxidase) from Trametopsis cervina has an exposed catalytic tyrosine residue (Tyr(181)) instead of the tryptophan conserved in other lignin-degrading peroxidases. Pristine LiP showed a lag period in VA (veratryl alcohol) oxidation. However, VA-LiP (LiP after treatment with H2O2 and VA) lacked this lag, and H2O2-LiP (H2O2-treated LiP) was inactive. MS analyses revealed that VA-LiP includes one VA molecule covalently bound to the side chain of Tyr(181), whereas H2O2-LiP contains a hydroxylated Tyr(181). No adduct is formed in the Y171N variant. Molecular docking showed that VA binding is favoured by sandwich pi stacking with Tyr(181) and Phe(89). EPR spectroscopy after peroxide activation of the pre-treated LiPs showed protein radicals other than the tyrosine radical found in pristine LiP, which were assigned to a tyrosine VA adduct radical in VA-LiP and a dihydroxyphenyalanine radical in H2O2-LiP. Both radicals are able to oxidize large low-redox-potential substrates, but H2O2-LiP is unable to oxidize high-redox-potential substrates. Transient-state kinetics showed that the tyrosine VA adduct strongly promotes (>100-fold) substrate oxidation by compound LE, the rate-limiting step in catalysis. The novel activation mechanism is involved in ligninolysis, as demonstrated using lignin model substrates. The present paper is the first report on autocatalytic modification, resulting in functional alteration, among class II peroxidases. C1 [Miki, Yuta; Fernandez-Fueyo, Elena; Fernandez, Maria I.; de los Rios, Vivian; Ruiz-Duenas, Francisco J.; Martinez, Angel T.] CSIC, Ctr Invest Biol, E-28040 Madrid, Spain. [Pogni, Rebecca; Baratto, Maria Camilla; Sinicropi, Adalgisa; Basosi, Riccardo] Univ Siena, Dept Biotechnol Chem & Pharm, I-53100 Siena, Italy. [Acebes, Sandra; Lucas, Fatima; Guallar, Victor] Barcelona Supercomp Ctr, Joint BSC IRB Res Program Computat Biol, E-08034 Barcelona, Spain. [Hammel, Kenneth E.] USDA Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. [Guallar, Victor] Passeig Lluis Co 23, ICREA, E-08010 Barcelona, Spain. RP Martinez, AT (reprint author), CSIC, Ctr Invest Biol, Ramiro de Maeztu 9, E-28040 Madrid, Spain. EM ATMartinez@cib.csic.es RI Hammel, Kenneth/G-1890-2011; Sinicropi, Adalgisa/D-6096-2011; Ruiz-Duenas, Francisco/L-9837-2015; Guallar, Victor/B-1579-2013; Basosi, Riccardo/E-7497-2011; OI Lucas, Maria Fatima/0000-0001-8672-9940; Hammel, Kenneth/0000-0002-2935-5847; Sinicropi, Adalgisa/0000-0001-5605-6482; Ruiz-Duenas, Francisco/0000-0002-9837-5665; Guallar, Victor/0000-0002-4580-1114; Basosi, Riccardo/0000-0003-1026-7668; acebes, sandra/0000-0001-8854-654X; Martinez, Angel T/0000-0002-1584-2863 FU ProteoRed platform of the Spanish Institute of Health Carlos III (ISCIII); Italian MIUR; European project BIORENEW [NMP2-CT-2006-026456]; European project PELE [ERC-2009-Adg 25027]; European project PEROXICATS [KBBE-2010-4-265397]; U.S. Department of Energy [DE-AI02-07ER64480]; Spanish Ministry of Economy and Competitiveness; [BIO2008-01533]; [CTQ2010-18123]; [BIO2011-26694] FX This work was supported by the Spanish projects BIO2008-01533 (to A.T.M.), CTQ2010-18123 (to V.G.) and BIO2011-26694 (to F.J.R.-D.) and the ProteoRed platform of the Spanish Institute of Health Carlos III (ISCIII); the Italian MIUR (PRIN 2009) project (to R.P. and R.B.); the European projects BIORENEW [grant number NMP2-CT-2006-026456 (to A.T.M.)], PELE [grant number ERC-2009-Adg 25027 (to V.G)] and PEROXICATS [grant number KBBE-2010-4-265397 (to A.T.M.)]; and the U.S. Department of Energy [grant number DE-AI02-07ER64480 (to K.E.H.). Y.M. and F.J.R.-D. thank the financial support of a European project contract, and a Ramon y Cajal contract of the Spanish Ministry of Economy and Competitiveness respectively. NR 32 TC 9 Z9 9 U1 0 U2 39 PU PORTLAND PRESS LTD PI LONDON PA THIRD FLOOR, EAGLE HOUSE, 16 PROCTER STREET, LONDON WC1V 6 NX, ENGLAND SN 0264-6021 J9 BIOCHEM J JI Biochem. J. PD JUN 15 PY 2013 VL 452 BP 575 EP 584 DI 10.1042/BJ20130251 PN 3 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA 161SR UT WOS:000320214100019 PM 23548202 ER PT J AU Vazquez-Cintron, E Pellett, S Tepp, WH Stanker, LH Band, PA Johnson, EA Ichtchenko, K AF Vazquez-Cintron, E. Pellett, S. Tepp, W. H. Stanker, L. H. Band, P. A. Johnson, E. A. Ichtchenko, K. TI Neuronal targeting, internalization and biological activity of a recombinant atoxic derivative of botulinum neurotoxin A SO TOXICON LA English DT Meeting Abstract CT 7th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Toxins (TOXINS) CY OCT 02-05, 2011 CL Santa Fe, NM C1 [Vazquez-Cintron, E.; Band, P. A.; Ichtchenko, K.] NYU, Sch Med, Dept Pharmacol, New York, NY USA. [Pellett, S.; Tepp, W. H.; Johnson, E. A.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Stanker, L. H.] ARS, USDA, Albany, CA USA. [Band, P. A.] New York Univ Hosp Joint Dis, Dept Orthopaed Surg, New York, NY USA. EM ichtck01@med.nyu.edu OI Ichtchenko, Konstantin/0000-0002-2198-1325 NR 0 TC 1 Z9 1 U1 0 U2 0 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD JUN 15 PY 2013 VL 68 BP 77 EP 77 DI 10.1016/j.toxicon.2012.07.060 PG 1 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA 159VN UT WOS:000320075500048 ER PT J AU Cheng, LW Stanker, LH Henderson, TD Scotcher, MC AF Cheng, L. W. Stanker, L. H. Henderson, T. D., II Scotcher, M. C. TI Antibody protection of mice from botulinum neurotoxin serotype B intoxication SO TOXICON LA English DT Meeting Abstract CT 7th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Toxins (TOXINS) CY OCT 02-05, 2011 CL Santa Fe, NM C1 [Cheng, L. W.; Stanker, L. H.; Henderson, T. D., II] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. [Scotcher, M. C.] Genencor, Palo Alto, CA USA. EM luisa.cheng@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD JUN 15 PY 2013 VL 68 BP 83 EP 84 DI 10.1016/j.toxicon.2012.07.074 PG 2 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA 159VN UT WOS:000320075500062 ER PT J AU Rasooly, R Do, PM AF Rasooly, R. Do, P. M. TI Simultaneous detection and differentiation of botulinum toxin serotypes A and B by internally-quenched fluorogenic peptides SO TOXICON LA English DT Meeting Abstract CT 7th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Toxins (TOXINS) CY OCT 02-05, 2011 CL Santa Fe, NM C1 [Rasooly, R.; Do, P. M.] USDA, ARS, Western Reg Res Ctr, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD JUN 15 PY 2013 VL 68 BP 94 EP 95 DI 10.1016/j.toxicon.2012.07.101 PG 2 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA 159VN UT WOS:000320075500089 ER PT J AU Sun, Z Lou, J Cheng, L Smith, TJ Dong, J Wen, W Conrad, F Garcia, C Fan, Y Henderson, T Brown, J Skerry, J Smith, LA Harrison, T D'Andrea, A Tomic, M Marks, JD AF Sun, Z. Lou, J. Cheng, L. Smith, T. J. Dong, J. Wen, W. Conrad, F. Garcia, C. Fan, Y. Henderson, T. Brown, J. Skerry, J. Smith, L. A. Harrison, T. D'Andrea, A. Tomic, M. Marks, J. D. TI Comparison of neutralizing abilities of human monoclonal antibodies binding different epitopes on botulinum neurotoxin A SO TOXICON LA English DT Meeting Abstract CT 7th International Conference on Basic and Therapeutic Aspects of Botulinum and Tetanus Toxins (TOXINS) CY OCT 02-05, 2011 CL Santa Fe, NM C1 [Sun, Z.; Lou, J.; Dong, J.; Wen, W.; Conrad, F.; Garcia, C.; Fan, Y.; Marks, J. D.] Univ Calif San Francisco, Dept Anesthesia, San Francisco, CA 94143 USA. [Cheng, L.; Henderson, T.] ARS, Western Reg Res Ctr, USDA, Albany, CA USA. [Brown, J.; Skerry, J.] USA, Med Inst Infect Dis, Integrated Toxinol Div, Ft Detrick, MD USA. [Smith, T. J.; Smith, L. A.] USA, Med Res & Mat Command, Ft Detrick, MD USA. [Harrison, T.; D'Andrea, A.] SRI Int Biosci, Menlo Pk, CA USA. [Tomic, M.] XOMA LLC, Berkeley, CA USA. EM sunz@anesthesia.ucsf.edu NR 0 TC 0 Z9 0 U1 0 U2 5 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD JUN 15 PY 2013 VL 68 BP 98 EP 99 DI 10.1016/j.toxicon.2012.07.110 PG 2 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA 159VN UT WOS:000320075500098 ER PT J AU Cho, J Bosch, D Vellidis, G Lowrance, R Strickland, T AF Cho, Jaepil Bosch, David Vellidis, George Lowrance, Richard Strickland, Timothy TI Multi-site evaluation of hydrology component of SWAT in the coastal plain of southwest Georgia SO HYDROLOGICAL PROCESSES LA English DT Article DE multi-site evaluation; ungauged watershed; regionalization; parameter extrapolation; spatial distribution; modelling ID SIMULATION-MODELS; WATERSHEDS; RAINFALL AB Simulation of watershed scale hydrologic and water quality processes is important for watershed assessments. Proper characterization of the accuracy of these simulations, particularly in cases with limited observed data, is critical. The Soil & Water Assessment Tool (SWAT) is frequently used for watershed scale simulation. The accuracy of the model was assessed by extrapolating calibration results from a well studied Coastal Plain watershed in Southwest Georgia, USA, to watersheds within the same geographic region without further calibration. SWAT was calibrated and validated on a 16.7-km2 subwatershed within the Little River Experimental Watershed by varying six model parameters. The optimized parameter set was then applied to a watershed of similar land use and soils, a smaller watershed with different land use and soils and three larger watersheds within the same drainage system without further calibration. Simulation results with percent bias (PB) +/- 15%PB<+/- 25% and Nash-Sutcliffe efficiency (NSE) 0.50150 mu g l(-1)). Results demonstrate that the new approach will be suitable for quantifying phycocyanin concentration in cyanobacteria dominated turbid productive waters. (c) 2013 Elsevier Inc. All rights reserved. C1 [Mishra, Sachidananda] Mississippi State Univ, Geosyst Res Inst, Mississippi State, MS 39762 USA. [Mishra, Sachidananda] Mississippi State Univ, Dept Geosci, Mississippi State, MS 39762 USA. [Mishra, Deepak R.] Univ Georgia, Dept Geog, Athens, GA 30602 USA. [Lee, Zhongping] Univ Massachusetts, Dept Environm Earth & Ocean Sci, Boston, MA 02125 USA. [Tucker, Craig S.] USDA ARS, Catfish Genet Res Unit, Stoneville, MS 38776 USA. RP Mishra, S (reprint author), Dept Geosci, 108 Hilbun Hall,POB 5448, Mississippi State, MS 39762 USA. EM sm1287@msstate.edu RI Mishra, Sachi/R-3412-2016 NR 46 TC 25 Z9 27 U1 3 U2 70 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD JUN 15 PY 2013 VL 133 BP 141 EP 151 DI 10.1016/j.rse.2013.02.004 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA 130TD UT WOS:000317942100012 ER PT J AU Kanobe, MN Rodermel, SR Bailey, T Scott, MP AF Kanobe, Milly N. Rodermel, Steven R. Bailey, Theodore Scott, M. Paul TI Changes in endogenous gene transcript and protein levels in maize plants expressing the soybean ferritin transgene SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE soybean ferritin; transgenic maize; endogenous genes; gene expression; transcription; iron ID TOBACCO OVEREXPRESSING FERRITIN; IRON ACCUMULATION; ASPERGILLUS PHYTASE; WHEAT-GRAIN; RICE; NICOTIANAMINE; INHIBITION; MUTANT; IDENTIFICATION; ACQUISITION AB Transgenic agricultural crops with increased nutritive value present prospects for contributing to public health. However, their acceptance is poor in many countries due to the perception that genetic modification may cause unintended effects on expression of native genes in the host plant. Here, we tested effects of soybean ferritin transgene (SoyFer1, M64337) on transcript and protein levels of endogenous genes in maize. Results showed that the transgene was successfully introduced and expressed in the maize seed endosperm. mRNA abundance of seven tested iron homeostasis genes and seed storage protein genes differed significantly between seed samples positive and negative for the transgene. The PCR negative samples had higher zein and total protein content compared to the positive samples. However, PCR positive samples had significantly higher concentrations of calcium, magnesium, and iron. We have shown that the soybean ferritin transgene affected the expression of native iron homeostasis genes in the maize plant. These results underscore the importance of taking a holistic approach to the evaluation of transgenic events in target plants, comparing the transgenic plant to the untransformed controls. C1 [Kanobe, Milly N.; Scott, M. Paul] Iowa State Univ, Dept Agron, Ames, IA USA. [Rodermel, Steven R.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA USA. [Bailey, Theodore] Iowa State Univ, Dept Stat, Ames, IA USA. [Scott, M. Paul] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Scott, MP (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, 1407 Agron Hall, Ames, IA 50011 USA. EM paul.scott@ars.usda.gov FU Rockefeller Foundation [2004 FS 060]; USDA-NIFA grant; CSREES grant FX Financial support for this research came from the Rockefeller Foundation, Grant 2004 FS 060 and USDA-NIFA and CSREES grants, to which the authors are very grateful. The authors are also thankful to Dr. Manju Reddy, Iowa State University for providing the soybean ferritin protein antibody for initial screening. NR 46 TC 7 Z9 7 U1 4 U2 9 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 14 PY 2013 VL 4 AR 196 DI 10.3389/fpls.2013.00196 PG 14 WC Plant Sciences SC Plant Sciences GA 296DY UT WOS:000330166500001 PM 23785377 ER PT J AU Matsumoto, C Matthan, NR Wilk, JB Lichtenstein, AH Gaziano, JM Djousse, L AF Matsumoto, Chisa Matthan, Nirupa R. Wilk, Jemma B. Lichtenstein, Alice H. Gaziano, J. Michael Djousse, Luc TI Erythrocyte stearidonic acid and other n-3 fatty acids and CHD in the Physicians' Health Study SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Stearidonic acid; n-3 Fatty acids; alpha-Linolenic acid; CHD ID ALPHA-LINOLENIC ACID; MYOCARDIAL-INFARCTION; CARDIOVASCULAR-DISEASE; CORONARY-HEART; RISK-FACTORS; FINAL REPORT; PREVENTION; CONVERSION; MORTALITY; FAILURE AB Intake of marine-based n-3 fatty acids (EPA, docosapentaenoic acid and DHA) is recommended to prevent CHD. Stearidonic acid (SDA), a plant-based n-3 fatty acid, is a precursor of EPA and may be more readily converted to EPA than alpha-linolenic acid (ALA). While transgenic soyabeans might supply SDA at low cost, it is unclear whether SDA is associated with CHD risk. Furthermore, associations of other n-3 fatty acids with CHD risk remain inconsistent. The present ancillary study examined the association of erythrocyte SDA as well as other n-3 fatty acids with the risk of CHD. In a prospective nested case-control study of the Physicians' Health Study, we randomly selected 1000 pairs of incident CHD with matching controls. Erythrocyte fatty acids were measured using GC. We used conditional logistic regression to estimate relative risks. Mean age was 68.7 (SD 8.7) years. In a multivariable model controlling for matching factors and established CHD risk factors, OR for CHD for each standard deviation increase of log-SDA was 1.03 (95% CI 0.90, 1.18). Corresponding values for log-ALA and log-marine n-3 fatty acids were 1.04 (95% CI 0.94, 1.16) and 0.97 (95% CI 0.88, 1.07), respectively. In conclusion, the present data did not show an association among erythrocyte SDA, ALA or marine n-3 fatty acids and the risk of CHD in male physicians. C1 [Matsumoto, Chisa; Wilk, Jemma B.; Gaziano, J. Michael; Djousse, Luc] Brigham & Womens Hosp, Div Aging, Boston, MA 02120 USA. [Matsumoto, Chisa; Wilk, Jemma B.; Gaziano, J. Michael; Djousse, Luc] Harvard Univ, Sch Med, Boston, MA USA. [Matthan, Nirupa R.; Lichtenstein, Alice H.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Gaziano, J. Michael; Djousse, Luc] Boston Vet Affairs Healthcare Syst, Boston, MA USA. [Gaziano, J. Michael] Brigham & Womens Hosp, Div Prevent Med, Boston, MA 02115 USA. RP Matsumoto, C (reprint author), Brigham & Womens Hosp, Div Aging, Boston, MA 02120 USA. EM cmatsumoto2@partners.org FU National Heart, Lung, and Blood Institute Bethesda, MD [R21 HL088081, HL-26490, HL-34595]; National Cancer Institute [CA-34944, CA-40360, CA-097193] FX We are indebted to the participants in the PHS for their outstanding commitment and cooperation and also thank the entire PHS staff. The present ancillary study was supported by grant R21 HL088081 (L. D.) from the National Heart, Lung, and Blood Institute Bethesda, MD. The PHS is supported by grants CA-34944, CA-40360 and CA-097193 from the National Cancer Institute and grants HL-26490 and HL-34595 from the National Heart, Lung, and Blood Institute, Bethesda, MD. The author contributions are as follows: L. D. contributed to the design and the conception of the study. The measurements of SFA were taken by A. H. L. and N. R. M. The statistical analyses were conducted by C. M., J. B. W. and L. D. C. M. and L. D. The first draft of the manuscript was written by All the authors contributed to the review of the manuscript for scientific content. L. D. obtained the funding for the present study and also supervised the entire study. The sponsors of the study had no role in the design and conduct of the study; collection, management, analysis and interpretation of the data; and preparation, review or approval of the manuscript. The authors declare no conflict of interest. NR 31 TC 0 Z9 0 U1 1 U2 16 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 J9 BRIT J NUTR JI Br. J. Nutr. PD JUN 14 PY 2013 VL 109 IS 11 BP 2044 EP 2049 DI 10.1017/S0007114512004060 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA 146WT UT WOS:000319126700015 PM 23098619 ER PT J AU Crooijmans, RPMA Fife, MS Fitzgerald, TW Strickland, S Cheng, HH Kaiser, P Redon, R Groenen, MAM AF Crooijmans, Richard P. M. A. Fife, Mark S. Fitzgerald, Tomas W. Strickland, Shurnevia Cheng, Hans H. Kaiser, Pete Redon, Richard Groenen, Martien A. M. TI Large scale variation in DNA copy number in chicken breeds SO BMC GENOMICS LA English DT Article DE Copy number variation; Chicken; aCGH; Line-specific CNVs; Inter-specific CNVs; Genes ID HUMAN GENOME; STRUCTURAL VARIATION; MAREKS-DISEASE; EVOLUTION; MAP; GENES; RESISTANCE; FEATHER; LOCI; PCR AB Background: Detecting genetic variation is a critical step in elucidating the molecular mechanisms underlying phenotypic diversity. Until recently, such detection has mostly focused on single nucleotide polymorphisms (SNPs) because of the ease in screening complete genomes. Another type of variant, copy number variation (CNV), is emerging as a significant contributor to phenotypic variation in many species. Here we describe a genome-wide CNV study using array comparative genomic hybridization (aCGH) in a wide variety of chicken breeds. Results: We identified 3,154 CNVs, grouped into 1,556 CNV regions (CNVRs). Thirty percent of the CNVs were detected in at least 2 individuals. The average size of the CNVs detected was 46.3 kb with the largest CNV, located on GGAZ, being 4.3 Mb. Approximately 75% of the CNVs are copy number losses relatively to the Red Jungle Fowl reference genome. The genome coverage of CNVRs in this study is 60 Mb, which represents almost 5.4% of the chicken genome. In particular large gene families such as the keratin gene family and the MHC show extensive CNV. Conclusions: A relative large group of the CNVs are line-specific, several of which were previously shown to be related to the causative mutation for a number of phenotypic variants. The chance that inter-specific CNVs fall into CNVRs detected in chicken is related to the evolutionary distance between the species. Our results provide a valuable resource for the study of genetic and phenotypic variation in this phenotypically diverse species. C1 [Crooijmans, Richard P. M. A.; Groenen, Martien A. M.] Wageningen Univ, Anim Breeding & Genom Ctr, NL-6700 AH Wageningen, Netherlands. [Fife, Mark S.] Pirbright Inst, Genet & Genom Grp, Compton Lab, Compton RG20 7NN, Berks, England. [Fitzgerald, Tomas W.; Redon, Richard] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England. [Redon, Richard] Univ Nantes, Inst Thorax, UMR Inserm CNRS 6291 1087, F-44007 Nantes 1, France. [Strickland, Shurnevia] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19717 USA. [Cheng, Hans H.] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Kaiser, Pete] Univ Edinburgh, Roslin Inst, Easter Bush EH25 9RG, Midlothian, Scotland. [Kaiser, Pete] Univ Edinburgh, R D SVS, Easter Bush EH25 9RG, Midlothian, Scotland. RP Crooijmans, RPMA (reprint author), Wageningen Univ, Anim Breeding & Genom Ctr, POB 338, NL-6700 AH Wageningen, Netherlands. EM richard.crooijmans@wur.nl RI Redon, Richard/F-3303-2014; Institute, Pirbright/K-4476-2014; Groenen, Martien/D-8408-2012; Crooijmans, Richard/E-2492-2012; OI Redon, Richard/0000-0001-7751-2280; Groenen, Martien/0000-0003-0484-4545; Fitzgerald, Tomas/0000-0002-2370-8496 FU European Union [FOOD-CT-2004-506416]; BBSRC Institute Strategic Programme Grant at the Pirbright Institute; BBSRC Institute Strategic Programme Grant at Roslin Institute; BBSRC Institute Strategic Programme Grant at Hendrix Genetics, The Netherlands; Cobb-Vantress Inc, USA; University of Delaware, College of Agriculture and Natural Resources Seed [ANSC462123] FX This work was financially supported by European Union grant FOOD-CT-2004-506416 (EADGENE), BBSRC Institute Strategic Programme Grants at the Pirbright Institute and the Roslin Institute, Hendrix Genetics, The Netherlands, Cobb-Vantress Inc, USA and The University of Delaware, College of Agriculture and Natural Resources Seed grant ANSC462123. We would like to thank Carl J. Schmidt and Mary E. Delany for their contribution of DNA from some of the lines and Bert Dibbits for the help in performing CNV validation. NR 36 TC 15 Z9 16 U1 1 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 13 PY 2013 VL 14 AR 398 DI 10.1186/1471-2164-14-398 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 212FU UT WOS:000323968900001 PM 23763846 ER PT J AU Utsunomiya, YT do Carmo, AS Carvalheiro, R Neves, HHR Matos, MC Zavarez, LB O'Brien, AMP Solkner, J McEwan, JC Cole, JB Van Tassell, CP Schenkel, FS da Silva, MVGB Neto, LRP Sonstegard, TS Garcia, JF AF Utsunomiya, Yuri T. do Carmo, Adriana S. Carvalheiro, Roberto Neves, Haroldo H. R. Matos, Marcia C. Zavarez, Ludmilla B. O'Brien, Ana M. Perez Soelkner, Johann McEwan, John C. Cole, John B. Van Tassell, Curtis P. Schenkel, Flavio S. da Silva, Marcos V. G. B. Porto Neto, Laercio R. Sonstegard, Tad S. Garcia, Jose F. TI Genome-wide association study for birth weight in Nellore cattle points to previously described orthologous genes affecting human and bovine height SO BMC GENETICS LA English DT Article DE GWAS; Birth weight; Bos primigenius indicus; Nellore cattle; Stature ID QUANTITATIVE TRAIT LOCI; BEEF-CATTLE; BIOLOGICAL PATHWAYS; GESTATION LENGTH; GROWTH TRAITS; CALVING EASE; PLAG1; REGRESSION; POPULATION; IDENTIFICATION AB Background: Birth weight (BW) is an economically important trait in beef cattle, and is associated with growthand stature-related traits and calving difficulty. One region of the cattle genome, located on Bos primigenius taurus chromosome 14 (BTA14), has been previously shown to be associated with stature by multiple independent studies, and contains orthologous genes affecting human height. A genome-wide association study (GWAS) for BW in Brazilian Nellore cattle (Bos primigenius indicus) was performed using estimated breeding values (EBVs) of 654 progeny-tested bulls genotyped for over 777,000 single nucleotide polymorphisms (SNPs). Results: The most significant SNP (rs133012258, P-GC = 1.34 x 10(-9)), located at BTA14: 25376827, explained 4.62% of the variance in BW EBVs. The surrounding 1 Mb region presented high identity with human, pig and mouse autosomes 8, 4 and 4, respectively, and contains the orthologous height genes PLAG1, CHCHD7, MOS, RPS20, LYN, RDHE2 (SDR16C5) and PENK. The region also overlapped 28 quantitative trait loci (QTLs) previously reported in literature by linkage mapping studies in cattle, including QTLs for birth weight, mature height, carcass weight, stature, pre-weaning average daily gain, calving ease, and gestation length. Conclusions: This study presents the first GWAS applying a high-density SNP panel to identify putative chromosome regions affecting birth weight in Nellore cattle. These results suggest that the QTLs on BTA14 associated with body size in taurine cattle (Bos primigenius taurus) also affect birth weight and size in zebu cattle (Bos primigenius indicus). C1 [Utsunomiya, Yuri T.; do Carmo, Adriana S.; Neves, Haroldo H. R.; Matos, Marcia C.; Zavarez, Ludmilla B.] UNESP, Univ Estadual Paulista, Fac Ciencias Agr Vet, BR-14884900 Jaboticabal, SP, Brazil. [Carvalheiro, Roberto] GenSys Consultores Assoc, BR-90680000 Porto Alegre, RS, Brazil. [O'Brien, Ana M. Perez; Soelkner, Johann] BOKU Univ Nat Resources & Life Sci, Dept Sustainable Agr Syst, Div Livestock Sci, Vienna, Austria. [McEwan, John C.] Ctr Reprod & Genom, AgRes, Invermay, Mosgiel, New Zealand. [Cole, John B.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. [Van Tassell, Curtis P.; Sonstegard, Tad S.] USDA, Agr Res Serv, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Schenkel, Flavio S.] Univ Guelph, Ctr Genet Improvement Livestock, Guelph, ON N1G 2W1, Canada. [da Silva, Marcos V. G. B.] Embrapa Dairy Cattle, Bioinformat & Anim Genom Lab, Juiz De Fora, MG, Brazil. [Porto Neto, Laercio R.] Univ Queensland, Sch Vet Sci, Gatton, Qld 4343, Australia. [Porto Neto, Laercio R.] Univ New England, Sch Rural & Environm Sci, Armidale, NSW 2351, Australia. [Garcia, Jose F.] UNESP, Univ Estadual Paulista, Fac Med Vet Aracatuba, BR-16050680 Aracatuba, SP, Brazil. RP Garcia, JF (reprint author), UNESP, Univ Estadual Paulista, Fac Med Vet Aracatuba, BR-16050680 Aracatuba, SP, Brazil. EM jfgarcia@fmva.unesp.br RI Porto-Neto, Laercio/D-2594-2012; de Rezende Neves, Haroldo Henrique/F-9521-2012; Utsunomiya, Yuri Tani/K-5214-2013; Cole, John/J-8571-2014; McEwan, John/A-1143-2008; OI Porto-Neto, Laercio/0000-0002-3536-8265; Utsunomiya, Yuri Tani/0000-0002-6526-8337; Cole, John/0000-0003-1242-4401; McEwan, John/0000-0003-4801-6207; Carvalheiro, Roberto/0000-0002-4506-0555; Van Tassell, Curtis/0000-0002-8416-2087 FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [560922/2010-8, 483590/2010-0]; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/16643-2, 2010/52030-2]; Next-Generation BioGreen 21 Program [PJ008196]; Rural Development Administration, Republic of Korea FX We thank Guilherme Penteado Coelho Filho and Daniel Biluca for technical assistance in sample acquisition. This research was supported by: Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) - process 560922/2010-8 and 483590/2010-0; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) - process 2011/16643-2 and 2010/52030-2; Next-Generation BioGreen 21 Program (No. PJ008196), Rural Development Administration, Republic of Korea. NR 49 TC 33 Z9 35 U1 1 U2 25 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUN 13 PY 2013 VL 14 AR 52 DI 10.1186/1471-2156-14-52 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA 163YP UT WOS:000320374900001 PM 23758625 ER PT J AU McKimm-Breschkin, JL Barrett, S Pudjiatmoko Azhar, M Wong, FYK Selleck, P Mohr, PG McGrane, J Kim, M AF McKimm-Breschkin, Jennifer L. Barrett, Susan Pudjiatmoko Azhar, Muhammad Wong, Frank Y. K. Selleck, Paul Mohr, Peter G. McGrane, James Kim, Mia TI I222 Neuraminidase Mutations Further Reduce Oseltamivir Susceptibility of Indonesian Clade 2.1 Highly Pathogenic Avian Influenza A(H5N1) Viruses SO PLOS ONE LA English DT Article ID A H5N1 VIRUSES; SIALIC-ACID; H1N1 VIRUS; 2.2-A RESOLUTION; A(H1N1) VIRUS; DRUG DESIGN; B VIRUSES; IN-VITRO; RESISTANT; INHIBITORS AB We have tested the susceptibility to neuraminidase inhibitors of 155 clade 2.1 H5N1 viruses from Indonesia, isolated between 2006-2008 as well as 12 clade 1 isolates from Thailand and Cambodia from 2004-2007 using a fluorometric MUNANA-based enzyme inhibition assay. The Thailand and Cambodian clade 1 isolates tested here were all susceptible to oseltamivir and zanamivir, and sequence comparison indicated that reduced oseltamivir susceptibility we observed previously with clade 1 Cambodian isolates correlated with an S246G neuraminidase mutation. Eight Indonesian viruses (5%), all bearing I222 neuraminidase mutations, were identified as mild to extreme outliers for oseltamivir based on statistical analysis by box plots. IC(50)s were from 50 to 500-fold higher than the reference clade 1 virus from Viet Nam, ranging from 43-75 nM for I222T/V mutants and from 268-349 nM for I222M mutants. All eight viruses were from different geographic locales; all I222M variants were from central Sumatra. None of the H5N1 isolates tested demonstrated reduced susceptibility to zanamivir (IC(50)s all <5 nM). All I222 mutants showed loss of slow binding specifically for oseltamivir in an IC50 kinetics assay. We identified four other Indonesian isolates with higher IC(50)s which also demonstrated loss of slow binding, including one virus with an I117V mutation. There was a minimal effect on the binding of zanamivir and peramivir for all isolates tested. As H5N1 remains a potential pandemic threat, the incidence of mutations conferring reduced oseltamivir susceptibility is concerning and emphasizes the need for greater surveillance of drug susceptibility. C1 [McKimm-Breschkin, Jennifer L.; Barrett, Susan] Commonwealth Sci & Ind Res Org, Parkville, Vic, Australia. [McKimm-Breschkin, Jennifer L.; Barrett, Susan; Wong, Frank Y. K.; Selleck, Paul; Mohr, Peter G.] Commonwealth Sci & Ind Res Org, Australian Anim Hlth Lab, Geelong, Vic, Australia. [Pudjiatmoko; Azhar, Muhammad] Minist Agr, Directorate Anim Hlth, Directorate Gen Livestock, Jakarta, Indonesia. [Pudjiatmoko; Azhar, Muhammad] Minist Agr, Anim Hlth Serv, Jakarta, Indonesia. [McGrane, James; Kim, Mia] UN, Food & Agr Org, Emergency Ctr Transboundary Anim Dis, Jakarta, Indonesia. [Kim, Mia] World Org Anim Hlth & Food, Paris, France. [Kim, Mia] United Nations Global Network Expertise Anim Infl, Agr Org, OFFLU, Paris, France. [Kim, Mia] World Org Anim Hlth & Food, Rome, Italy. [Kim, Mia] United Nations Global Network Expertise Anim Infl, Agr Org, OFFLU, Rome, Italy. [Kim, Mia] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. RP McKimm-Breschkin, JL (reprint author), Commonwealth Sci & Ind Res Org, Parkville, Vic, Australia. EM mck245@csiro.au RI Wong, Frank/H-1950-2013; McKimm-Breschkin, Jennifer/D-1880-2013; OI Barrett, Susan/0000-0003-1132-5268 FU Australian Department of Agriculture, Forestry and Fisheries FX Parts of this work were supported by the Australian Department of Agriculture, Forestry and Fisheries. No additional external funding was received for this study. The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript. NR 57 TC 11 Z9 11 U1 0 U2 10 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 11 PY 2013 VL 8 IS 6 AR e66105 DI 10.1371/journal.pone.0066105 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 169CU UT WOS:000320755400110 PM 23776615 ER PT J AU Bradford, SA Torkzaban, S Shapiro, A AF Bradford, Scott A. Torkzaban, Saeed Shapiro, Alexander TI A Theoretical Analysis of Colloid Attachment and Straining in Chemically Heterogeneous Porous Media SO LANGMUIR LA English DT Article ID IONIC-STRENGTH; PHYSICOCHEMICAL FILTRATION; FACILITATED TRANSPORT; TANGENTIAL FORCES; SOLID-SURFACE; PLANE WALL; PARTICLES; DEPOSITION; DETACHMENT; ADHESION AB A balance of applied hydrodynamic (T-H) and resisting adhesive (TA) torques was conducted over a chemically heterogeneous porous medium that contained random roughness of height h(r) to determine the fraction of the solid surface area that contributes to colloid immobilization (S-f*) under unfavorable attachment conditions. This model considers resistance due to deformation and the horizontal component of the adhesive force (F-AT), spatial variations in the pore scale velocity distribution, and the influence of h(r) on lever arms for T-H and T-A. Values of S-f* were calculated for a wide range of physicochemical properties to gain insight into mechanisms and factors influencing colloid immobilization. Colloid attachment processes were demonstrated to depend on solution ionic strength (IS), the colloid radius (r(c)), the Young's modulus (K), the amount of chemical heterogeneity (P+), and the Darcy velocity (q). Colloid immobilization was also demonstrated to occur on a rough surface in the absence of attachment In this case, S-f* depended on IS, r(c), the roughness fraction (f(0)), h(r), and q. Roughness tended to enhance T-A and diminish T-H. Consequently, the effect of IS on S-f* was enhanced by hr relative to attachment In contrast, the effects of r(c) and q on S-f* were diminished by h(r) in comparison to attachment. Colloid immobilization adjacent to macroscopic roughness locations shares many similarities to grain-grain contact points and may be viewed as a type of straining process. In general, attachment was more important for higher IS and variance in the secondary minimum, and for smaller r(c), q, and K, but diffusion decreased these values. Conversely, straining was dominant for the opposite conditions. Discrepancies in the literature on mechanisms of colloid retention are likely due to a lack of consideration of all of these factors. C1 [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. [Torkzaban, Saeed] CSIRO Land & Water, Glen Osmond, SA 5064, Australia. [Shapiro, Alexander] Tech Univ Denmark, Dept Chem & Biochem Engn, Ctr Energy Resources Engn, DK-2800 Lyngby, Denmark. RP Bradford, SA (reprint author), ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. EM Scott.Bradford@ars.usda.gov RI Torkzaban, Saeed/G-7377-2013 OI Torkzaban, Saeed/0000-0002-5146-9461 FU USDA, ARS [NP 214] FX This research was supported by the USDA, ARS, NP 214. The USDA is an equal opportunity provider and employer. NR 61 TC 29 Z9 29 U1 3 U2 50 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0743-7463 J9 LANGMUIR JI Langmuir PD JUN 11 PY 2013 VL 29 IS 23 BP 6944 EP 6952 DI 10.1021/la4011357 PG 9 WC Chemistry, Multidisciplinary; Chemistry, Physical; Materials Science, Multidisciplinary SC Chemistry; Materials Science GA 165LM UT WOS:000320485400021 PM 23687981 ER PT J AU Fry, TL VanDalen, KK Shriner, SA Moore, SM Hanlon, CA VerCauteren, KC AF Fry, Tricia L. VanDalen, Kaci K. Shriner, Susan A. Moore, Susan M. Hanlon, Cathleen A. VerCauteren, Kurt C. TI Humoral immune response to oral rabies vaccination in raccoon kits: Problems and implications SO VACCINE LA English DT Article DE Raccoon kits; Oral rabies vaccination; Procyon lotor; Rabies; Raboral V-RG; Maternal antibodies ID RECOMBINANT VIRUS-VACCINE; PROCYON-LOTOR; UNITED-STATES; FOX CUBS; PROTECTION; ANTIBODIES; SURVEILLANCE; EFFICACY; BAITS AB Little is known about the immunogenicity of RABORAL V-RG (R) (V-RG), an oral rabies vaccine, in raccoon kits (Procyon lotor). The objectives of this study were to characterize the immunogenicity of V-RG in young kits and investigate the potential impact of maternal antibodies on response to vaccination of nursing raccoon kits. Raccoon kits (n = 30) were vaccinated at either 3 weeks of age, 7 weeks of age, or assigned as contact controls. Nineteen kits (73%) that were whelped by unvaccinated mothers responded to V-RG exposure (orally or indirect contact) by production of detectable rabies virus neutralizing antibodies (RVNA) while 7 (27%) kits did not respond to V-RG exposure. Four kits were whelped by a mother with high levels of RVNA and all four kits acquired maternal rabies antibodies. At approximately 9 months of age, all kits were inoculated with a killed rabies vaccine, IMRAB3 (R). The kits which initially responded to V-RG oral vaccination or contact with vaccinated littermates demonstrated a rapid anamnestic response. In contrast, the V-RG non-responders and those with acquired maternal antibodies exhibited a primary immune response to IMRAB3 (R), where RVNA levels were substantially lower on days 5 and 7 than the levels in the animals with an anamnestic response. These findings suggest that the nave contact kits and the nonresponsive kits most likely remained susceptible to rabies virus infection whereas the ones demonstrating response to V-RG would not have been susceptible to a rabies virus infection. Published by Elsevier Ltd. C1 [Fry, Tricia L.; VanDalen, Kaci K.; Shriner, Susan A.; VerCauteren, Kurt C.] Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Moore, Susan M.; Hanlon, Cathleen A.] Kansas State Univ, Rabies Lab, Manhattan, KS 66502 USA. RP VanDalen, KK (reprint author), Wildlife Serv, USDA, Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, 4101 Laporte Ave, Ft Collins, CO 80521 USA. EM tricia.l.fry@aphis.usda.gov; kaci.vandalen@aphis.usda.gov; susan.a.shriner@aphis.usda.gov; smoore@vet.k-state.edu; chanlon@vet.k-state.edu; kurt.c.vercauteren@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182 NR 31 TC 4 Z9 4 U1 0 U2 9 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X J9 VACCINE JI Vaccine PD JUN 10 PY 2013 VL 31 IS 26 BP 2811 EP 2815 DI 10.1016/j.vaccine.2013.04.016 PG 5 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA 168ZD UT WOS:000320745600005 PM 23602534 ER PT J AU Zhang, Y Liu, SK Lu, JG Jiang, YL Gao, XY Ninwichian, P Li, C Waldbieser, G Liu, ZJ AF Zhang, Yu Liu, Shikai Lu, Jianguo Jiang, Yanliang Gao, Xiaoyu Ninwichian, Parichart Li, Chao Waldbieser, Geoff Liu, Zhanjiang TI Comparative genomic analysis of catfish linkage group 8 reveals two homologous chromosomes in zebrafish and other teleosts with extensive inter-chromosomal rearrangements SO BMC GENOMICS LA English DT Article DE Comparative mapping; Synteny; Genome; Chromosome; Linkage map; Physical map; Catfish; Fish ID BAC-END SEQUENCES; BASS DICENTRARCHUS-LABRAX; TROUT ONCORHYNCHUS-MYKISS; RADIATION HYBRID MAP; SALMON SALMO-SALAR; NILE TILAPIA GENOME; MODEL FISH GENOMES; SPARUS-AURATA L; CHANNEL CATFISH; ICTALURUS-PUNCTATUS AB Background: Comparative genomics is a powerful tool to transfer genomic information from model species to related non-model species. Channel catfish (Ictalurus punctatus) is the primary aquaculture species in the United States. Its existing genome resources such as genomic sequences generated from next generation sequencing, BAC end sequences (BES), physical maps, linkage maps, and integrated linkage and physical maps using BES-associated markers provide a platform for comparative genomic analysis between catfish and other model teleost fish species. This study aimed to gain understanding of genome organizations and similarities among catfish and several sequenced teleost genomes using linkage group 8 (LG8) as a pilot study. Results: With existing genome resources, 287 unique genes were identified in LG8. Comparative genome analysis indicated that most of these 287 genes on catfish LG8 are located on two homologous chromosomes of zebrafish, medaka, stickleback, and three chromosomes of green-spotted pufferfish. Large numbers of conserved syntenies were identified. Detailed analysis of the conserved syntenies in relation to chromosome level similarities revealed extensive inter-chromosomal and intra-chromosomal rearrangements during evolution. Of the 287 genes, 35 genes were found to be duplicated in the catfish genome, with the vast majority of the duplications being interchromosomal. Conclusions: Comparative genome analysis is a powerful tool even in the absence of a well-assembled whole genome sequence. In spite of sequence stacking due to low resolution of the linkage and physical maps, conserved syntenies can be identified although the exact gene order and orientation are unknown at present. Through chromosome-level comparative analysis, homologous chromosomes among teleosts can be identified. Syntenic analysis should facilitate annotation of the catfish genome, which in turn, should facilitate functional inference of genes based on their orthology. C1 [Zhang, Yu; Liu, Shikai; Lu, Jianguo; Jiang, Yanliang; Gao, Xiaoyu; Ninwichian, Parichart; Li, Chao; Liu, Zhanjiang] Auburn Univ, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA. [Zhang, Yu; Liu, Shikai; Lu, Jianguo; Jiang, Yanliang; Gao, Xiaoyu; Ninwichian, Parichart; Li, Chao; Liu, Zhanjiang] Auburn Univ, Fish Mol Genet & Biotechnol Lab, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA. [Waldbieser, Geoff] ARS, Catfish Genet Res Unit, USDA, Stoneville, MS 38776 USA. RP Liu, ZJ (reprint author), Auburn Univ, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA. EM liuzhan@auburn.edu RI Liu, Shikai /D-4664-2013 OI Liu, Shikai /0000-0001-5777-489X FU Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture (NIFA) [2009-35205-05101, 2010-65205-20356, 2012-67015 -19410]; China Scholarship Council (CSC) FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2009-35205-05101, 2010-65205-20356 and 2012-67015 -19410 from the USDA National Institute of Food and Agriculture (NIFA). Thanks are given to Alabama Supercomputer Center for providing the computer capacity for the bioinformatic analysis. Yu Zhang, Shikai Liu and Chao Li are supported by a scholarship from the China Scholarship Council (CSC) for studying abroad. NR 83 TC 10 Z9 10 U1 0 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD JUN 10 PY 2013 VL 14 AR 387 DI 10.1186/1471-2164-14-387 PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 169WH UT WOS:000320810900001 PM 23758806 ER PT J AU Rich, JO Leathers, TD Anderson, AM Bischoff, KM Manitchotpisit, P AF Rich, Joseph O. Leathers, Timothy D. Anderson, Amber M. Bischoff, Kenneth M. Manitchotpisit, Pennapa TI Laccases from Aureobasidium pullulans SO ENZYME AND MICROBIAL TECHNOLOGY LA English DT Article DE Aureobasidium pullulans; Glycosylation; Laccase; Thermostability; Trametes versicolor; Pycnoporus cinnabarinus ID AROMATIC-COMPOUNDS; FUNGAL LACCASES; GREEN CHEMISTRY; COLOR VARIANTS; PURIFICATION; OXIDATION; XYLANASE; ENZYMES; GROWTH; LIGNIN AB Laccases are polyphenol oxidases (EC 1.10.3.2) that have numerous industrial and bioremediation applications. Laccases are well known as lignin-degrading enzymes, but these enzymes can play numerous other roles in fungi. In this study, 41 strains of the fungus Aureobasidium pullulans were examined for laccase production. Enzymes from A. pullulans were distinct from those from lignin-degrading fungi and associated with pigment production. Laccases from strains in phylogenetic clade 5, which produced a dark vinaceous pigment, exhibited a temperature optimum of 50-60 degrees C and were stable for an hour at 50 degrees C, unlike enzymes from the lignin-degrading fungi Trametes versicolor and Pycnoporus cinnabarinus. Laccase purified from A. pullulans strain NRRL 50381, a representative of clade 5, was glycosylated but had a molecular weight of 60-70 kDa after Endo H treatment. Laccase purified from strain NRRL Y-2568, which produced a dark olivaceous pigment, was also glycosylated, but had a molecular weight of greater than 100 kDa after Endo H treatment. Published by Elsevier Inc. C1 [Rich, Joseph O.; Leathers, Timothy D.; Anderson, Amber M.; Bischoff, Kenneth M.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Manitchotpisit, Pennapa] Rangsit Univ, Fac Sci, Dept Med Sci, Biochem Unit, Pathum Thani 12000, Thailand. RP Rich, JO (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM joseph.rich@ars.usda.gov; tim.leathers@ars.usda.gov NR 38 TC 7 Z9 7 U1 0 U2 23 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0141-0229 J9 ENZYME MICROB TECH JI Enzyme Microb. Technol. PD JUN 10 PY 2013 VL 53 IS 1 BP 33 EP 37 DI 10.1016/j.enzmictec.2013.03.015 PG 5 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA 163QN UT WOS:000320352100006 PM 23683702 ER PT J AU Ammar, ED Alessandro, R Shatters, RG Hall, DG AF Ammar, El-Desouky Alessandro, Rocco Shatters, Robert G., Jr. Hall, David G. TI Behavioral, Ultrastructural and Chemical Studies on the Honeydew and Waxy Secretions by Nymphs and Adults of the Asian Citrus Psyllid Diaphorina citri (Hemiptera: Psyllidae) SO PLOS ONE LA English DT Article ID HOST-PLANTS; GREENING DISEASE; FINE-STRUCTURE; SCALE INSECT; SURFACE WAX; PHLOEM-SAP; CICADELLIDAE; HOMOPTERA; STERNORRHYNCHA; PSEUDOCOCCIDAE AB The Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae) is the primary vector of the bacterium causing citrus huanglongbing (citrus greening), the most serious disease of citrus worldwide. Psyllids and other hemipterans produce large amounts of honeydew, which has been used previously as an indicator of phloem sap composition and insect feeding or metabolism. Behavioral, ultrastructural and chemical studies on ACP, its honeydew and waxy secretions showed important differences between nymphs, males and females, and suggested some mechanisms by which the psyllids, especially nymphs and adult females, can minimize their contamination with honeydew excretions. The anal opening in ACP, near the posterior end of the abdomen, is on the ventral side in nymphs and on the dorsal side in adult males and females. Video recordings showed that adult males produce clear sticky droplets of honeydew gently deposited behind their body on the leaf surface, whereas adult females produce whitish honeydew pellets powerfully propelled away from the female body, probably to get their excretions away from eggs and newly hatched nymphs. ACP nymphs produce long ribbons or tubes of honeydew that frequently stay attached to the exuviae after molting, or drop when feeding on the lower side of citrus leaves. Furthermore, honeydew excretions of both nymphs and adult females are covered with a thin layer of whitish waxy material ultrastructurally composed of a convoluted network of long fine filaments or ribbons. This material is extruded from intricate arrays of wax pores in the circumanal ring (around the anus) that is found in nymphs and females but not in males of ACP or other psyllid species. Infrared microscopy and mass spectroscopy revealed that, in addition to various sugars, honeydew excretions of ACP nymphs and females are covered with a thin layer of wax similar in profile to ester waxes. C1 [Ammar, El-Desouky; Alessandro, Rocco; Shatters, Robert G., Jr.; Hall, David G.] ARS, USDA, Hort Res Lab, Ft Pierce, FL USA. RP Ammar, ED (reprint author), ARS, USDA, Hort Res Lab, Ft Pierce, FL USA. EM Eldesouky.ammar@ars.usda.gov OI Alessandro, Rocco/0000-0001-7983-0557 FU Florida Citrus Research Foundation FX Funding was provided by the Florida Citrus Research Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 5 Z9 6 U1 4 U2 43 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2013 VL 8 IS 6 AR e64938 DI 10.1371/journal.pone.0064938 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 164VY UT WOS:000320440500011 PM 23762268 ER PT J AU Blackburn, MB Martin, PAW Kuhar, D Farrar, RR Gundersen-Rindal, DE AF Blackburn, Michael B. Martin, Phyllis A. W. Kuhar, Daniel Farrar, Robert R., Jr. Gundersen-Rindal, Dawn E. TI Phylogenetic Distribution of Phenotypic Traits in Bacillus thuringiensis Determined by Multilocus Sequence Analysis SO PLOS ONE LA English DT Article ID CEREUS GROUP; POPULATION-STRUCTURE; BACTERIA; STRAINS; EVOLUTION; SURVIVAL AB Diverse isolates from a world-wide collection of Bacillus thuringiensis were classified based on phenotypic profiles resulting from six biochemical tests; production of amylase (T), lecithinase (L), urease (U), acid from sucrose (S) and salicin (A), and the hydrolysis of esculin (E). Eighty two isolates representing the 15 most common phenotypic profiles were subjected to phylogenetic analysis by multilocus sequence typing; these were found to be distributed among 19 sequence types, 8 of which were novel. Approximately 70% of the isolates belonged to sequence types corresponding to the classical B. thuringiensis varieties kurstaki (20 isolates), finitimus (15 isolates), morrisoni (11 isolates) and israelensis (11 isolates). Generally, there was little apparent correlation between phenotypic traits and phylogenetic position, and phenotypic variation was often substantial within a sequence type. Isolates of the sequence type corresponding to kurstaki displayed the greatest apparent phenotypic variation with 6 of the 15 phenotypic profiles represented. Despite the phenotypic variation often observed within a given sequence type, certain phenotypes appeared highly correlated with particular sequence types. Isolates with the phenotypic profiles TLUAE and LSAE were found to be exclusively associated with sequence types associated with varieties kurstaki and finitimus, respectively, and 7 of 8 TS isolates were found to be associated with the morrisoni sequence type. Our results suggest that the B. thuringiensis varieties israelensis and kurstaki represent the most abundant varieties of Bt in soil. C1 [Blackburn, Michael B.; Martin, Phyllis A. W.; Kuhar, Daniel; Farrar, Robert R., Jr.; Gundersen-Rindal, Dawn E.] USDA, Invas Insect Biocontrol & Behav Lab, Agr Res Serv, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Blackburn, MB (reprint author), USDA, Invas Insect Biocontrol & Behav Lab, Agr Res Serv, Henry A Wallace Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. EM mike.blackburn@ars.usda.gov FU Agricultural Research Service [1245-22000-273-00] FX Research funded by the Agricultural Research Service, Project Number: 1245-22000-273-00. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 20 TC 2 Z9 2 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2013 VL 8 IS 6 AR e66061 DI 10.1371/journal.pone.0066061 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 164VY UT WOS:000320440500061 PM 23762464 ER PT J AU Gaines, TA Wright, AA Molin, WT Lorentz, L Riggins, CW Tranel, PJ Beffa, R Westra, P Powles, SB AF Gaines, Todd A. Wright, Alice A. Molin, William T. Lorentz, Lothar Riggins, Chance W. Tranel, Patrick J. Beffa, Roland Westra, Philip Powles, Stephen B. TI Identification of Genetic Elements Associated with EPSPS Gene Amplification SO PLOS ONE LA English DT Article ID 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; INSECTICIDE RESISTANCE; TRANSPOSABLE ELEMENTS; GLYPHOSATE RESISTANCE; ARABIDOPSIS-THALIANA; LTR-RETROTRANSPOSONS; EVOLUTION; PLANTS; EXPRESSION; HERBICIDE AB Weed populations can have high genetic plasticity and rapid responses to environmental selection pressures. For example, 100-fold amplification of the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene evolved in the weed species Amaranthus palmeri to confer resistance to glyphosate, the world's most important herbicide. However, the gene amplification mechanism is unknown. We sequenced the EPSPS gene and genomic regions flanking EPSPS loci in A. palmeri, and searched for mobile genetic elements or repetitive sequences. The EPSPS gene was 10,229 bp, containing 8 exons and 7 introns. The gene amplification likely proceeded through a DNA-mediated mechanism, as introns exist in the amplified gene copies and the entire amplified sequence is at least 30 kb in length. Our data support the presence of two EPSPS loci in susceptible (S) A. palmeri, and that only one of these was amplified in glyphosate-resistant (R) A. palmeri. The EPSPS gene amplification event likely occurred recently, as no sequence polymorphisms were found within introns of amplified EPSPS copies from R individuals. Sequences with homology to miniature inverted-repeat transposable elements (MITEs) were identified next to EPSPS gene copies only in R individuals. Additionally, a putative Activator (Ac) transposase and a repetitive sequence region were associated with amplified EPSPS genes. The mechanism controlling this DNA-mediated amplification remains unknown. Further investigation is necessary to determine if the gene amplification may have proceeded via DNA transposon-mediated replication, and/or unequal recombination between different genomic regions resulting in replication of the EPSPS gene. C1 [Gaines, Todd A.; Powles, Stephen B.] Univ Western Australia, Sch Plant Biol, Australian Herbicide Resistance Initiat, Crawley, WA, Australia. [Gaines, Todd A.; Lorentz, Lothar; Beffa, Roland] Bayer CropSci, Weed Resistance Res, Frankfurt, Germany. [Wright, Alice A.; Molin, William T.] USDA, Crop Prod Syst Res Unit, Agr Res Serv, Stoneville, MS USA. [Riggins, Chance W.; Tranel, Patrick J.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Westra, Philip] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. RP Gaines, TA (reprint author), Univ Western Australia, Sch Plant Biol, Australian Herbicide Resistance Initiat, Crawley, WA, Australia. EM todd.gaines@uwa.edu.au RI Gaines, Todd/A-8998-2008; OI Gaines, Todd/0000-0003-1485-7665; Tranel, Patrick/0000-0003-0666-4564 FU United States Department of Agriculture-Agricultural Research Service (USDA-ARS) [6402-21000-050-00D] FX This work was supported in part by United States Department of Agriculture-Agricultural Research Service (USDA-ARS) project number 6402-21000-050-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. No additional external funding was received for this study. NR 46 TC 21 Z9 22 U1 0 U2 66 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 10 PY 2013 VL 8 IS 6 AR e65819 DI 10.1371/journal.pone.0065819 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 164VY UT WOS:000320440500051 PM 23762434 ER PT J AU Lindqvist-Kreuze, H Cho, K Portal, L Rodriguez, F Simon, R Mueller, LA Spooner, DM Bonierbale, M AF Lindqvist-Kreuze, Hannele Cho, Kwangsoo Portal, Leticia Rodriguez, Flor Simon, Reinhard Mueller, Lukas A. Spooner, David M. Bonierbale, Merideth TI Linking the potato genome to the conserved ortholog set (COS) markers SO BMC GENETICS LA English DT Article DE Conserved ortholog set (COS); Genetic mapping; Potato genome; Solanum ID LATE BLIGHT RESISTANCE; QUANTITATIVE TRAIT LOCI; PHYTOPHTHORA-INFESTANS; SOLANUM-TUBEROSUM; FIELD-RESISTANCE; QTL ANALYSIS; LINKAGE MAP; GENE; TOMATO; SOLANACEAE AB Background: Conserved ortholog set (COS) markers are an important functional genomics resource that has greatly improved orthology detection in Asterid species. A comprehensive list of these markers is available at Sol Genomics Network (http://solgenomics.net/) and many of these have been placed on the genetic maps of a number of solanaceous species. Results: We amplified over 300 COS markers from eight potato accessions involving two diploid landraces of Solanum tuberosum Andigenum group (formerly classified as S. goniocalyx, S. phureja), and a dihaploid clone derived from a modern tetraploid cultivar of S. tuberosum and the wild species S. berthaultii, S. chomatophilum, and S. paucissectum. By BLASTn (Basic Local Alignment Search Tool of the NCBI, National Center for Biotechnology Information) algorithm we mapped the DNA sequences of these markers into the potato genome sequence. Additionally, we mapped a subset of these markers genetically in potato and present a comparison between the physical and genetic locations of these markers in potato and in comparison with the genetic location in tomato. We found that most of the COS markers are single-copy in the reference genome of potato and that the genetic location in tomato and physical location in potato sequence are mostly in agreement. However, we did find some COS markers that are present in multiple copies and those that map in unexpected locations. Sequence comparisons between species show that some of these markers may be paralogs. Conclusions: The sequence-based physical map becomes helpful in identification of markers for traits of interest thereby reducing the number of markers to be tested for applications like marker assisted selection, diversity, and phylogenetic studies. C1 [Lindqvist-Kreuze, Hannele; Cho, Kwangsoo; Portal, Leticia; Rodriguez, Flor; Simon, Reinhard; Bonierbale, Merideth] Int Potato Ctr, Lima, Peru. [Rodriguez, Flor; Spooner, David M.] Univ Wisconsin, USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. [Mueller, Lukas A.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Lindqvist-Kreuze, H (reprint author), Int Potato Ctr, POB 5969, Lima, Peru. EM h.lindqvist-kreuze@cgiar.org OI Simon, Reinhard/0000-0002-4608-9077 FU USDA National Research Initiative grant [2008-35300-18669] FX This work was supported by the USDA National Research Initiative grant number 2008-35300-18669) to DS, MB, and LM. NR 52 TC 3 Z9 3 U1 0 U2 25 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUN 8 PY 2013 VL 14 AR 51 DI 10.1186/1471-2156-14-51 PG 12 WC Genetics & Heredity SC Genetics & Heredity GA 169ZY UT WOS:000320820500001 PM 23758607 ER PT J AU Cochran, SD Cole, JB Null, DJ Hansen, PJ AF Cochran, Sarah D. Cole, John B. Null, Daniel J. Hansen, Peter J. TI Discovery of single nucleotide polymorphisms in candidate genes associated with fertility and production traits in Holstein cattle SO BMC GENETICS LA English DT Article DE Daughter pregnancy rate; Fertility; Dairy cattle; SNP; Candidate gene ID GROWTH-FACTOR-I; EPITHELIAL-MESENCHYMAL TRANSITION; GENOME-WIDE ASSOCIATION; HORMONE-RECEPTOR GENE; HUMAN CUMULUS CELLS; DAIRY-COWS; UNITED-STATES; BOVINE OOCYTES; DEVELOPMENTAL COMPETENCE; PREIMPLANTATION EMBRYOS AB Background: Identification of single nucleotide polymorphisms (SNPs) for specific genes involved in reproduction might improve reliability of genomic estimates for these low-heritability traits. Semen from 550 Holstein bulls of high (>= 1.7; n = 288) or low (<= -2; n = 262) daughter pregnancy rate (DPR) was genotyped for 434 candidate SNPs using the Sequenom MassARRAY (R) system. Three types of SNPs were evaluated: SNPs previously reported to be associated with reproductive traits or physically close to genetic markers for reproduction, SNPs in genes that are well known to be involved in reproductive processes, and SNPs in genes that are differentially expressed between physiological conditions in a variety of tissues associated in reproductive function. Eleven reproduction and production traits were analyzed. Results: A total of 40 SNPs were associated (P < 0.05) with DPR. Among these were genes involved in the endocrine system, cell signaling, immune function and inhibition of apoptosis. A total of 10 genes were regulated by estradiol. In addition, 22 SNPs were associated with heifer conception rate, 33 with cow conception rate, 36 with productive life, 34 with net merit, 23 with milk yield, 19 with fat yield, 13 with fat percent, 19 with protein yield, 22 with protein percent, and 13 with somatic cell score. The allele substitution effect for SNPs associated with heifer conception rate, cow conception rate, productive life and net merit were in the same direction as for DPR. Allele substitution effects for several SNPs associated with production traits were in the opposite direction as DPR. Nonetheless, there were 29 SNPs associated with DPR that were not negatively associated with production traits. Conclusion: SNPs in a total of 40 genes associated with DPR were identified as well as SNPs for other traits. It might be feasible to include these SNPs into genomic tests of reproduction and other traits. The genes associated with DPR are likely to be important for understanding the physiology of reproduction. Given the large number of SNPs associated with DPR that were not negatively associated with production traits, it should be possible to select for DPR without compromising production. C1 [Cochran, Sarah D.; Hansen, Peter J.] Univ Florida, Dept Anim Sci, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA. [Cochran, Sarah D.; Hansen, Peter J.] Univ Florida, Genet Inst, Gainesville, FL 32611 USA. [Cole, John B.; Null, Daniel J.] USDA ARS, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. RP Hansen, PJ (reprint author), Univ Florida, Dept Anim Sci, DH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USA. EM Hansen@animal.ufl.edu RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Florida Dairy Checkoff Program; Agriculture and Food Research Initiative from the USDA National Institute of Food and Agriculture [2013-68004, 2013-20365] FX Research was supported by the Florida Dairy Checkoff Program and by Agriculture and Food Research Initiative Competitive Grant no. 2013-68004 -20365 from the USDA National Institute of Food and Agriculture. The authors thank the USDA CDDR and USDA National Animal Germplasm Program for donating semen used in the current study, Taurus Service Inc. for reducing the price of semen, and Maureen Long (University of Florida) for assistance with the IPA analysis. NR 144 TC 16 Z9 17 U1 2 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUN 7 PY 2013 VL 14 AR 49 DI 10.1186/1471-2156-14-49 PG 23 WC Genetics & Heredity SC Genetics & Heredity GA 167FX UT WOS:000320617800001 PM 23759029 ER PT J AU Trona, F Anfora, G Balkenius, A Bengtsson, M Tasin, M Knight, A Janz, N Witzgall, P Ignell, R AF Trona, Federica Anfora, Gianfranco Balkenius, Anna Bengtsson, Marie Tasin, Marco Knight, Alan Janz, Niklas Witzgall, Peter Ignell, Rickard TI Neural coding merges sex and habitat chemosensory signals in an insect herbivore SO PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES LA English DT Article DE chemical communication; reproductive isolation; magic trait; intracellular recordings; functional imaging; Cydia pomonella ID MOTH CYDIA-POMONELLA; FEMALE CODLING MOTH; EUROPEAN CORN-BORER; ANTENNAL LOBE; PLANT ODOR; LASPEYRESIA-POMONELLA; OLFACTORY RECEPTOR; APPLE VOLATILES; HOST RACES; PHEROMONE AB Understanding the processing of odour mixtures is a focus in olfaction research. Through a neuroethological approach, we demonstrate that different odour types, sex and habitat cues are coded together in an insect herbivore. Stronger flight attraction of codling moth males, Cydia pomonella, to blends of female sex pheromone and plant odour, compared with single compounds, was corroborated by functional imaging of the olfactory centres in the insect brain, the antennal lobes (ALs). The macroglomerular complex (MGC) in the AL, which is dedicated to pheromone perception, showed an enhanced response to blends of pheromone and plant signals, whereas the response in glomeruli surrounding the MGC was suppressed. Intracellular recordings from AL projection neurons that transmit odour information to higher brain centres, confirmed this synergistic interaction in the MGC. These findings underscore that, in nature, sex pheromone and plant odours are perceived as an ensemble. That mating and habitat cues are coded as blends in the MGC of the AL highlights the dual role of plant signals in habitat selection and in premating sexual communication. It suggests that the MGC is a common target for sexual and natural selection in moths, facilitating ecological speciation. C1 [Trona, Federica; Balkenius, Anna; Bengtsson, Marie; Witzgall, Peter; Ignell, Rickard] SLU, Div Chem Ecol, Dept Plant Protect Biol, S-23053 Alnarp, Sweden. [Trona, Federica; Anfora, Gianfranco; Tasin, Marco] Fdn Edmund Mach, IASMA Res & Innovat Ctr, I-38010 Trento, Italy. [Knight, Alan] USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Janz, Niklas] Stockholm Univ, Dept Zool, S-10691 Stockholm, Sweden. RP Trona, F (reprint author), Max Planck Inst Chem Ecol, Dept Evolutionary Neuroethol, D-07745 Jena, Germany. EM federica.trona@slu.se RI Janz, Niklas/B-1756-2010; Witzgall, Peter/N-6429-2013; OI Janz, Niklas/0000-0002-6379-7905; Witzgall, Peter/0000-0002-4697-3380; ANFORA, Gianfranco/0000-0003-2545-1409 FU Formas; SLU; IASMA FX We thank Valerio Mazzoni for advice on statistical analysis, and Duane Larson, USDA, ARS, Wapato, WA for assistance with the flight tunnel bioassays. This research is part of the Linnaeus programme Insect Chemical Ecology, Ethology and Evolution (IC-E3) and financially supported by Formas, SLU and IASMA. NR 52 TC 16 Z9 16 U1 5 U2 66 PU ROYAL SOC PI LONDON PA 6-9 CARLTON HOUSE TERRACE, LONDON SW1Y 5AG, ENGLAND SN 0962-8452 J9 P ROY SOC B-BIOL SCI JI Proc. R. Soc. B-Biol. Sci. PD JUN 7 PY 2013 VL 280 IS 1760 AR 20130267 DI 10.1098/rspb.2013.0267 PG 8 WC Biology; Ecology; Evolutionary Biology SC Life Sciences & Biomedicine - Other Topics; Environmental Sciences & Ecology; Evolutionary Biology GA 131VH UT WOS:000318024600008 PM 23595270 ER PT J AU Utro, F Haiminen, N Livingstone, D Cornejo, OE Royaert, S Schnell, RJ Motamayor, JC Nkuhn, D Parida, L AF Utro, Filippo Haiminen, Niina Livingstone, Donald, III Cornejo, Omar E. Royaert, Stefan Schnell, Raymond J. Motamayor, Juan Carlos Nkuhn, David Parida, Laxmi TI iXora: exact haplotype inferencing and trait association SO BMC GENETICS LA English DT Article DE Haplotype; Phasing; Phenotype Association; Trait Association; QTL; Randomization ID WHOLE-GENOME ASSOCIATION; GENOTYPE DATA; PHASE AB Background: We address the task of extracting accurate haplotypes from genotype data of individuals of large F-1 populations for mapping studies. While methods for inferring parental haplotype assignments on large F-1 populations exist in theory, these approaches do not work in practice at high levels of accuracy. Results: We have designed iXora (Identifying crossovers and recombining alleles), a robust method for extracting reliable haplotypes of a mapping population, as well as parental haplotypes, that runs in linear time. Each allele in the progeny is assigned not just to a parent, but more precisely to a haplotype inherited from the parent. iXora shows an improvement of at least 15% in accuracy over similar systems in literature. Furthermore, iXora provides an easy-to-use, comprehensive environment for association studies and hypothesis checking in populations of related individuals. Conclusions: iXora provides detailed resolution in parental inheritance, along with the capability of handling very large populations, which allows for accurate haplotype extraction and trait association. iXora is available for non-commercial use from http://researcher.ibm.com/project/3430. C1 [Utro, Filippo; Haiminen, Niina; Parida, Laxmi] IBM TJ Watson Res, Computat Biol Ctr, Yorktown Hts, NY USA. [Livingstone, Donald, III; Royaert, Stefan; Schnell, Raymond J.; Nkuhn, David] USDA ARS SHRS, Miami, FL USA. [Cornejo, Omar E.] Stanford Univ, Stanford, CA 94305 USA. [Livingstone, Donald, III; Schnell, Raymond J.; Motamayor, Juan Carlos] MARS Inc, Miami, FL USA. RP Parida, L (reprint author), IBM TJ Watson Res, Computat Biol Ctr, Yorktown Hts, NY USA. EM parida@us.ibm.com FU MARS; IBM Research; MARS, Incorporated FX FU is partially funded by MARS, under the joint cacao project with IBM Research. OEC is partially funded by MARS, Incorporated. The authors would like to thank Dr. Seth Findley and Joseph Conrad Stack, and the anonymous reviewers for providing valuable comments on the manuscript. NR 17 TC 2 Z9 2 U1 0 U2 4 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD JUN 6 PY 2013 VL 14 AR 48 DI 10.1186/1471-2156-14-48 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA 183BM UT WOS:000321788600001 PM 23742238 ER PT J AU Cooley, MB Jay-Russell, M Atwill, ER Carychao, D Nguyen, K Quinones, B Patel, R Walker, S Swimley, M Pierre-Jerome, E Gordus, AG Mandrell, RE AF Cooley, Michael B. Jay-Russell, Michele Atwill, Edward R. Carychao, Diana Nguyen, Kimberly Quinones, Beatriz Patel, Ronak Walker, Samarpita Swimley, Michelle Pierre-Jerome, Edith Gordus, Andrew G. Mandrell, Robert E. TI Development of a Robust Method for Isolation of Shiga Toxin-Positive Escherichia coli (STEC) from Fecal, Plant, Soil and Water Samples from a Leafy Greens Production Region in California SO PLOS ONE LA English DT Article ID REAL-TIME PCR; HEMOLYTIC-UREMIC SYNDROME; POLYMERASE-CHAIN-REACTION; IMMUNOMAGNETIC-SEPARATION; MULTIPLEX PCR; CONTAMINATED MANURE; IRRIGATION WATER; VARIABLE-NUMBER; 5'-NUCLEASE PCR; UNITED-STATES AB During a 2.5-year survey of 33 farms and ranches in a major leafy greens production region in California, 13,650 produce, soil, livestock, wildlife, and water samples were tested for Shiga toxin (stx)-producing Escherichia coli (STEC). Overall, 357 and 1,912 samples were positive for E. coli O157:H7 (2.6%) or non-O157 STEC (14.0%), respectively. Isolates differentiated by O-typing ELISA and multilocus variable number tandem repeat analysis (MLVA) resulted in 697 O157: H7 and 3,256 non-O157 STEC isolates saved for further analysis. Cattle (7.1%), feral swine (4.7%), sediment (4.4%), and water (3.3%) samples were positive for E. coli O157: H7; 7/32 birds, 2/145 coyotes, 3/88 samples from elk also were positive. Non-O157 STEC were at approximately 5-fold higher incidence compared to O157 STEC: cattle (37.9%), feral swine (21.4%), birds (2.4%), small mammals (3.5%), deer or elk (8.3%), water (14.0%), sediment (12.3%), produce (0.3%) and soil adjacent to produce (0.6%). stx1, stx2 and stx1/stx2 genes were detected in 63%, 74% and 35% of STEC isolates, respectively. Subtilase, intimin and hemolysin genes were present in 28%, 25% and 79% of non-O157 STEC, respectively; 23% were of the "Top 60" O-types. The initial method was modified twice during the study revealing evidence of culture bias based on differences in virulence and O-antigen profiles. MLVA typing revealed a diverse collection of O157 and non-O157 STEC strains isolated from multiple locations and sources and O157 STEC strains matching outbreak strains. These results emphasize the importance of multiple approaches for isolation of non-O157 STEC, that livestock and wildlife are common sources of potentially virulent STEC, and evidence of STEC persistence and movement in a leafy greens production environment. C1 [Cooley, Michael B.; Carychao, Diana; Nguyen, Kimberly; Quinones, Beatriz; Walker, Samarpita; Swimley, Michelle; Mandrell, Robert E.] USDA ARS, Produce Safety & Microbiol Res Unit, Albany, CA USA. [Jay-Russell, Michele; Atwill, Edward R.] Univ Calif Davis, Sch Vet Med, Davis, CA 95616 USA. [Jay-Russell, Michele; Atwill, Edward R.] Univ Calif Davis, Western Inst Food Safety & Secur, Davis, CA 95616 USA. [Patel, Ronak] USDA ARS, Natl Clonal Germplasm Repository Citrus & Dates, Riverside, CA USA. [Pierre-Jerome, Edith] Univ Washington, Dept Biol, Seattle, WA 98195 USA. [Gordus, Andrew G.] Calif Dept Fish & Game, Fresno, CA USA. RP Cooley, MB (reprint author), USDA ARS, Produce Safety & Microbiol Res Unit, Albany, CA USA. EM michael.cooley@ars.usda.gov FU National Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2006-55212-16927, 2007-35212-18239]; USDA Agricultural Research Service CRIS [5325-42000-044, 5325-42000-045] FX This project was supported by National Research Initiative Competitive Grant nos. 2006-55212-16927 and 2007-35212-18239 from the USDA National Institute of Food and Agriculture and USDA Agricultural Research Service CRIS projects 5325-42000-044 and -045. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 79 TC 25 Z9 25 U1 5 U2 43 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 6 PY 2013 VL 8 IS 6 AR e65716 DI 10.1371/journal.pone.0065716 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 173SK UT WOS:000321099000096 PM 23762414 ER PT J AU Cornman, RS Lopez, D Evans, JD AF Cornman, Robert Scott Lopez, Dawn Evans, Jay D. TI Transcriptional Response of Honey Bee Larvae Infected with the Bacterial Pathogen Paenibacillus larvae SO PLOS ONE LA English DT Article ID AMERICAN FOULBROOD; APIS-MELLIFERA; DROSOPHILA-MELANOGASTER; EXTRACELLULAR-MATRIX; ANOPHELES-GAMBIAE; IMMUNE-RESPONSE; ZP DOMAIN; PROTEINS; DISEASE; GENES AB American foulbrood disease of honey bees is caused by the bacterium Paenibacillus larvae. Infection occurs per os in larvae and systemic infection requires a breaching of the host peritrophic matrix and midgut epithelium. Genetic variation exists for both bacterial virulence and host resistance, and a general immunity is achieved by larvae as they age, the basis of which has not been identified. To quickly identify a pool of candidate genes responsive to P. larvae infection, we sequenced transcripts from larvae inoculated with P. larvae at 12 hours post-emergence and incubated for 72 hours, and compared expression levels to a control cohort. We identified 75 genes with significantly higher expression and six genes with significantly lower expression. In addition to several antimicrobial peptides, two genes encoding peritrophic-matrix domains were also up-regulated. Extracellular matrix proteins, proteases/protease inhibitors, and members of the Osiris gene family were prevalent among differentially regulated genes. However, analysis of Drosophila homologs of differentially expressed genes revealed spatial and temporal patterns consistent with developmental asynchrony as a likely confounder of our results. We therefore used qPCR to measure the consistency of gene expression changes for a subset of differentially expressed genes. A replicate experiment sampled at both 48 and 72 hours post infection allowed further discrimination of genes likely to be involved in host response. The consistently responsive genes in our test set included a hymenopteran-specific protein tyrosine kinase, a hymenopteran specific serine endopeptidase, a cytochrome P450 (CYP9Q1), and a homolog of trynity, a zona pellucida domain protein. Of the known honey bee antimicrobial peptides, apidaecin was responsive at both time-points studied whereas hymenoptaecin was more consistent in its level of change between biological replicates and had the greatest increase in expression by RNA-seq analysis. C1 [Cornman, Robert Scott; Lopez, Dawn; Evans, Jay D.] USDA ARS, Bee Res Lab, Beltsville, MD USA. RP Cornman, RS (reprint author), USDA ARS, Bee Res Lab, Beltsville, MD USA. EM scott.cornman@gmail.com RI Evans, Jay/C-8408-2012 OI Evans, Jay/0000-0002-0036-4651 FU Coordinate Agricultural Project on Pollinator Health (USDA National Institute of Food and Agriculture) FX This project was supported by the Coordinate Agricultural Project on Pollinator Health (USDA National Institute of Food and Agriculture). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 47 TC 8 Z9 8 U1 4 U2 52 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 6 PY 2013 VL 8 IS 6 AR e65424 DI 10.1371/journal.pone.0065424 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 173SK UT WOS:000321099000066 PM 23762370 ER PT J AU Adamski, D Makinson, JR Brown, BT Wright, SA Pratt, PD Brown, JW AF Adamski, D. Makinson, J. R. Brown, B. T. Wright, S. A. Pratt, P. D. Brown, J. W. TI DESCRIPTION AND EVALUATION OF METHARMOSTIS MULTILINEATA (COSMOPTERIGIDAE) AND IDIOPHANTIS SOREUTA (GELECHIIDAE) (LEPIDOPTERA: GELECHIOIDEA) FOR BIOCONTROL OF DOWNY ROSE MYRTLE, RHODOMYRTUS TOMENTOSA (MYRTACEAE) SO JOURNAL OF THE LEPIDOPTERISTS SOCIETY LA English DT Article DE classical weed biological control; Gelechioidea; Hong Kong; immatures; life-history; Myrtaceae; Southeast Asia; Taxonomy; Thailand ID SUPERFAMILY AB Two species of Gelechioidea (Lepidoptera), Metharmostis multilineata Adamski, n. sp. (Cosmopterigidae), and Idiophantis soreuta Meyrick, 1906 (Gelechiidae), were collected in southeastern Asia for evaluation as potential biocontrol agents against downy rose myrtle, Rhodomyrtus tomentosa (Aiton) Hassk. (Myrtaceae), which has become an invasive weed in Florida, USA. Metharmostis Meyrick is reviewed and transferred from Yponomeutidae to Cosmopterigidae (Antequerinae). All life stages of M. multilineata are described and illustrated, with notes on its biology. In addition, protocols for rearing and host testing of M. multilineata are described in detail. Idiophantis appears to be associated with Myrtaceae, and the adult stage of I. soreuta is redescribed. Neither species was suitable for release in Florida. C1 [Adamski, D.] ARS, Systemat Entomol Lab, USDA, Beltsville, MD 20705 USA. [Makinson, J. R.; Brown, B. T.] CSIRO Ecosyst Sci, Australian Biol Control Lab, ARS, USDA, Brisbane, Qld 4001, Australia. [Wright, S. A.] ARS, USDA, Invas Plant Res Lab, Gainesville, FL 32608 USA. [Pratt, P. D.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. [Brown, J. W.] ARS, Systemat Entomol Lab, USDA, Beltsville, MD 20705 USA. RP Adamski, D (reprint author), ARS, Systemat Entomol Lab, USDA, Beltsville, MD 20705 USA. EM david.adamski@ars.usda.gov; jeff.makinson@csiro.au; bradley.brown@csiro.au; susan.wright@ars.usda.gov; paul.pratt@ars.usda.gov; john.brown@ars.usda.gov RI Makinson, Jeffrey/M-7031-2014 OI Makinson, Jeffrey/0000-0001-9870-5866 FU Florida Fish and Wildlife Conservation Committee, Tallahassee, FL, USA [099, 100]; USDA; ARS [58-6629-1-003, 58-6629-0-004, 58-6629-0-101]; South Florida Water Management District, West Palm Beach, FL [4600001959] FX Funding for this research and publication was provided by the Florida Fish and Wildlife Conservation Committee, Tallahassee, FL, USA, under the direction of D.C. Schmitz, Task Order # 099 and # 100, USDA, ARS Agreement # 58-6629-1-003 and # 58-6629-0-004, and by the South Florida Water Management District, West Palm Beach, FL, under the direction of L. Rodgers, Contract No. 4600001959, USDA, ARS Agreement No. 58-6629-0-101. NR 34 TC 5 Z9 6 U1 1 U2 3 PU LEPIDOPTERISTS SOC PI LOS ANGELES PA 900 EXPOSITION BLVD, LOS ANGELES, CA 90007-4057 USA SN 0024-0966 J9 J LEPID SOC JI J. Lepid. Soc. PD JUN 5 PY 2013 VL 67 IS 2 BP 111 EP 127 PG 17 WC Entomology SC Entomology GA 302KE UT WOS:000330602600002 ER PT J AU Krishnan, HB Chen, MH AF Krishnan, Hari B. Chen, Ming-Hsuan TI Identification of an Abundant 56 kDa Protein Implicated in Food Allergy as Granule-Bound Starch Synthase SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE allergen; anaphylaxis; granule-bound starch synthase; rice ID RICE ORYZA-SATIVA; MOLECULAR CHARACTERIZATION; POTENTIAL ALLERGENS; HYPOALLERGENIC RICE; BETA-CONGLYCININ; WAXY LOCUS; ASTHMA; GENE; EXPRESSION; GRAINS AB Rice, the staple food of south and east Asian counties, is considered to be hypoallergenic. However, several clinical studies have documented rice-induced allergy in sensitive patients. Rice proteins with molecular weights of 14-16, 26, 33, and 56 kDa have been identified as allergens. Recently, it was documented that the 56 kDa rice allergen was responsible for rice-induced anaphylaxis. The 14-16 kDa allergens have been identified as alpha-amylase inhibitors; the 26 kDa protein has been identified as alpha-globulin; and the 33 kDa protein has been identified as glyoxalase I. However, the identity of the 56 kDa rice allergen has not yet been determined. In this study, we demonstrate that serum from patients allergic to maize shows IgE binding to a 56 kDa protein that was present in both maize and rice but not in the oil seeds soybean and peanut. The 56 kDa IgE-binding protein was abundant in the rice endosperm. We have purified this protein from rice endosperm and demonstrated its reactivity to IgE antibodies from the serum of maize-allergic patients. The purified protein was subjected to matrix-assisted laser desorption ionization-time of flight-tandem mass spectrometry analysis, resulting in identification of this rice allergen as granule-bound starch synthase, a product of the Waxy gene. Immunoblot analysis using protein extracts from a waxy mutant of rice revealed the absence of the 56kDa IgE-binding protein. Our results demonstrate that the 56 kDa rice allergen is granule-bound starch synthase and raise the possibility of using waxy mutants of rice as a potential source of the hypoallergenic diet for patients sensitized to the 56 kDa rice allergen. C1 [Krishnan, Hari B.] Univ Missouri, Plant Genet Res Unit, ARS, USDA, Columbia, MO 65211 USA. [Chen, Ming-Hsuan] ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR 72160 USA. RP Krishnan, HB (reprint author), Univ Missouri, Plant Genet Res Unit, ARS, USDA, Columbia, MO 65211 USA. EM krishnan@ars.usda.gov NR 48 TC 4 Z9 7 U1 0 U2 13 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD JUN 5 PY 2013 VL 61 IS 22 BP 5404 EP 5409 DI 10.1021/jf4014372 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA 160XJ UT WOS:000320153500028 PM 23675783 ER PT J AU Bagramyan, K Kaplan, BE Cheng, LW Strotmeier, J Rummel, A Kalkum, M AF Bagramyan, Karine Kaplan, Bruce E. Cheng, Luisa W. Strotmeier, Jasmin Rummel, Andreas Kalkum, Markus TI Substrates and Controls for the Quantitative Detection of Active Botulinum Neurotoxin in Protease-Containing Samples SO ANALYTICAL CHEMISTRY LA English DT Article ID TOXIN TYPE-A; CLOSTRIDIUM-BOTULINUM; SEROTYPE-A; MASS-SPECTROMETRY; MECHANISM; TETANUS; ACID; PHARMACOKINETICS; PROTEOLYSIS; RECOGNITION AB Botulinum neurotoldns (BoNTs) are used in a wide variety of medical applications, but there is limited pharmacokinetic data on active BoNT. Monitoring BoNT activity in the circulation is challenging because BoNTs are highly toxic and are rapidly taken up by neurons and removed from the bloodstream. Previously we reported, a sensitive BoNT "Assay with a Large Immunosorbent Surface Area" that uses conversion of fluorogenic peptide substrates to measure the intrinsic endopeptidase activity of bead-captured BoNT However, in complex biological samples, protease contaminants can also cleave the substrates, reducing sensitivity and specificity of the assay. Here, we present a novel, set of, fluorogenic peptides that serve as BoNT-specific substrates and protease-sensitive controls. BoNT-cleavable substrates contain a C-terminal Nle, while BoNT-noncleavable controls contain its isomer epsilon-Ahx. The substrates are cleaved by BoNT subtypes Al-A3 an A5. Substrates and control peptides can be cleaved by non-BoNT proteases (e.g., trypsin, proteinase K, and thermolysin) while obeying Michaelis-Menten kinetics. Using this novel substrate/control set, we studied BoNT/A1 activity in two mouse models of botulism. We detected BoNT/A serum activities ranging from similar to 3600 to 10 amol/L., in blood of mice that had been intravenously injected 1 h prior with BoNT/A1 complex (100 to 4 pg/mouse). We also detected the endopeptidase activity of orally administered BoNT/A1 complex (1 mu g) in blood 5 h after administration; activity was greatest 7 h after administration. Redistribution and elevation rates for active toxin were measured and are comparable to those reported for inactive toxin. C1 [Bagramyan, Karine; Kaplan, Bruce E.; Kalkum, Markus] City Hope Natl Med Ctr, Beckman Res Inst, Dept Immunol, Duarte, CA 91010 USA. [Cheng, Luisa W.] USDA ARS, Foodborne Contaminants Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Strotmeier, Jasmin; Rummel, Andreas] Hannover Med Sch, Inst Toxikol, D-30623 Hannover, Germany. RP Kalkum, M (reprint author), City Hope Natl Med Ctr, Beckman Res Inst, Dept Immunol, Duarte, CA 91010 USA. EM mkalkum@coh.org RI Rummel, Andreas/I-7449-2013 FU National Institutes of Health [AI096169-01, AI065359-05]; United States Department of Agriculture, Agriculture Research Service, CRIS [5325-42000-048-00D]; Robert Koch Institut [1362/I-979] FX The authors thank Drs. James Marks and Jianlong Lou from the University of California, San Francisco, for providing the monoclonal anti-BoNT LC-A antibody 5A20.4. The study was funded by the National Institutes of Health through Grants AI096169-01 (M.K. and B.E.K.) and AI065359-05 (M.K., B.E.K., and L.W.C.) the United States Department of Agriculture, Agriculture Research Service, CRIS Project 5325-42000-048-00D (L.W.C.), and the Robert Koch Institut, 1362/I-979 (A.R. and J.S.). NR 56 TC 8 Z9 8 U1 1 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0003-2700 EI 1520-6882 J9 ANAL CHEM JI Anal. Chem. PD JUN 4 PY 2013 VL 85 IS 11 BP 5569 EP 5576 DI 10.1021/ac4008418 PG 8 WC Chemistry, Analytical SC Chemistry GA 160DJ UT WOS:000320096900042 PM 23656526 ER PT J AU Yuan, LX Zhu, YY Lin, ZQ Banuelos, G Li, W Yin, XB AF Yuan, Linxi Zhu, Yuanyuan Lin, Zhi-Qing Banuelos, Gary Li, Wei Yin, Xuebin TI A Novel Selenocystine-Accumulating Plant in Selenium-Mine Drainage Area in Enshi, China SO PLOS ONE LA English DT Article ID HYPERACCUMULATOR STANLEYA-PINNATA; PRAIRIE DOG HERBIVORY; INDIAN MUSTARD; MOLECULAR-MECHANISMS; ENDEMIC DISEASES; BRASSICA-JUNCEA; PROTECTS PLANTS; HUBEI PROVINCE; PHYTOREMEDIATION; ASTRAGALUS AB Plant samples of Cardamine hupingshanesis (Brassicaceae), Ligulariafischeri (Ledeb.) turcz (Steraceae) and their underlying top sediments were collected from selenium (Se) mine drainage areas in Enshi, China. Concentrations of total Se were measured using Hydride Generation-Atomic Fluorescence Spectrometry (HG-AFS) and Se speciation were determined using liquid chromatography/UV irradiation-hydride generation-atomic fluorescence spectrometry (LC-UV-HG-AFS). The results showed that C. hupingshanesis could accumulate Se to 239 +/- 201 mg/kg DW in roots, 316 +/- 184 mg/kg DW in stems, and 380 +/- 323 mg/kg DW in leaves, which identifies it as Se secondary accumulator. Particularly, it could accumulate Se up to 1965 +/- 271 mg/kg DW in leaves, 1787 +/- 167 mg/kg DW in stem and 4414 +/- 3446 mg/kg DW in roots, living near Se mine tailing. Moreover, over 70% of the total Se accumulated in C. hupingshanesis were in the form of selenocystine (SeCys(2)), increasing with increased total Se concentration in plant, in contrast to selenomethionine (SeMet) in non-accumulators (eg. Arabidopsis) and secondary accumulators (eg. Brassica juncea), and selenomethylcysteine (SeMeCys) in hyperaccumulators (eg. Stanleya pinnata). There is no convincing explanation on SeCys(2) accumulation in C. hupingshanesis based on current Se metabolism theory in higher plants, and further study will be needed. C1 [Yuan, Linxi; Zhu, Yuanyuan; Li, Wei; Yin, Xuebin] Jiangsu Bioengn Res Ctr Selenium, Suzhou, Jiangsu, Peoples R China. [Yuan, Linxi; Yin, Xuebin] Univ Sci & Technol China, Sch Earth & Space Sci, Hefei, Anhui, Peoples R China. [Lin, Zhi-Qing] So Illinois Univ, Environm Sci Program, Edwardsville, IL 62026 USA. [Lin, Zhi-Qing] So Illinois Univ, Dept Biol Sci, Edwardsville, IL 62026 USA. [Banuelos, Gary] USDA ARS, Parlier, CA USA. [Yuan, Linxi; Yin, Xuebin] Univ Sci & Technol China, Suzhou Inst Adv Study, Adv Lab Selenium & Human Hlth, Suzhou, Jiangsu, Peoples R China. RP Yuan, LX (reprint author), Jiangsu Bioengn Res Ctr Selenium, Suzhou, Jiangsu, Peoples R China. EM yuanli@mail.ustc.edu.cn; xbyin@ustc.edu.cn FU Natural Science Youth Foundation of Jiangsu Province for Youth, China [BK2012202]; Open Fund of State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences [Y052010020]; Open Fund of SOA Key Laboratory for Polar Science, Polar Research Institute of China [KP201106]; National Science Foundation of China [NSFC 40601088] FX This work was supported by Natural Science Youth Foundation of Jiangsu Province for Youth, China (BK2012202) (http://www.jsnsf.gov.cn/Index.aspx), Open Fund of State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences (Y052010020) (http://www.ssa.ac.cn/), Open Fund of SOA Key Laboratory for Polar Science, Polar Research Institute of China (KP201106) (http://www.pric.gov.cn/bmm.asp?bigid = 19), National Science Foundation of China (NSFC 40601088) (http://www.nsfc.gov.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 57 TC 9 Z9 10 U1 7 U2 60 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 4 PY 2013 VL 8 IS 6 AR e65615 DI 10.1371/journal.pone.0065615 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 158IE UT WOS:000319961900072 PM 23750270 ER PT J AU Lu, H Zhang, C Albrecht, U Shimizu, R Wang, GF Bowman, KD AF Lu, Hua Zhang, Chong Albrecht, Ute Shimizu, Rena Wang, Guanfeng Bowman, Kim D. TI Overexpression of a citrus NDR1 ortholog increases disease resistance in Arabidopsis SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE Pseudomonas syringae; salicylic acid; citrus canker; Huanglongbing; greening disease; Candidatus Liberibacter asiaticus; genetic engineering ID SYSTEMIC ACQUIRED-RESISTANCE; CANDIDATUS LIBERIBACTER-ASIATICUS; ACIBENZOLAR-S-METHYL; SALICYLIC-ACID; DEFENSE RESPONSES; PLANT DEFENSE; PSEUDOMONAS-SYRINGAE; HYPERSENSITIVE RESPONSE; TRANSCRIPTION FACTOR; SIGNALING PATHWAYS AB Emerging devastating diseases, such as Huanglongbing (HLB) and citrus canker, have caused tremendous losses to the citrus industry worldwide. Genetic engineering is a powerful approach that could allow us to increase citrus resistance against these diseases. The key to the success of this approach relies on a thorough understanding of defense mechanisms of citrus. Studies of Arabidopsis and other plants have provided a framework for us to better understand defense mechanisms of citrus. Salicylic acid (SA) is a key signaling molecule involved in basal defense and resistance (R) gene-mediated defense against broad-spectrum pathogens. The Arabidopsis gene NDR1 (NON-RACE-SPECIFIC DISEASE RESISTANCE 1) is a positive regulator of SA accumulation and is specifically required for signaling mediated by a subset of R genes upon recognition of their cognate pathogen effectors. Our bioinformatic analysis identified an ortholog of NDR1 from citrus, CsNDR1. Overexpression of CsNDR1 complemented susceptibility conferred by the Arabidopsis ndr1-1 mutant to Pseudomonas syringae strains and also led to enhanced resistance to an oomycete pathogen Hyaloperonospora arabidopsidis. Such heightened resistance is associated with increased SA production and expression of the defense marker gene PATHOGENESIS RELATED 1 (PR1). In addition, we found that expression of PR1 and accumulation of SA were induced to modest levels in citrus infected with Candidatus Liberibacter asiaticus, the bacterial pathogen associated with HLB disease. Thus, our data suggest that CsNDR1 is a functional ortholog of Arabidopsis NDR1. Since Ca. L. asiaticus infection only activates modest levels of defense responses in citrus, we propose that genetically increasing SA/NDR1-mediated pathways could potentially lead to enhanced resistance against HLB, citrus canker, and other destructive diseases challenging global citrus production. C1 [Lu, Hua; Zhang, Chong; Shimizu, Rena; Wang, Guanfeng] Univ Maryland Baltimore Cty, Dept Biol Sci, Baltimore, MD 21250 USA. [Albrecht, Ute; Bowman, Kim D.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL USA. RP Lu, H (reprint author), Univ Maryland Baltimore Cty, Dept Biol Sci, 1000 Hilltop Circle, Baltimore, MD 21250 USA. EM hualu@umbc.edu RI Luan, Gan/B-3211-2015; Zhang, Chong/J-6604-2014 FU Citrus Research and Development Foundation; China Scholarship Council FX We thank members in the Lu laboratory for assistance with this work. We thank Dr. Kevin Omland for assistance in phylogenetic analysis, Dr. William La Course for sharing his HPLC instrument, and Mr. Tim Ford for taking pictures for this publication. This work was supported by a grant from Citrus Research and Development Foundation to Hua Lu and a scholarship from China Scholarship Council to Chong Zhang. NR 74 TC 10 Z9 11 U1 2 U2 16 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD JUN 3 PY 2013 VL 4 AR 157 DI 10.3389/fpls.2013.00157 PG 10 WC Plant Sciences SC Plant Sciences GA 293QD UT WOS:000329987500001 PM 23761797 ER PT J AU Hogan, CS Mole, BM Grant, SR Willis, DK Charkowski, AO AF Hogan, Clifford S. Mole, Beth M. Grant, Sarah R. Willis, David K. Charkowski, Amy O. TI The Type III Secreted Effector DspE Is Required Early in Solanum tuberosum Leaf Infection by Pectobacterium carotovorum to Cause Cell Death, and Requires Wx((3-6))D/E Motifs SO PLOS ONE LA English DT Article ID ALTERNATIVE SIGMA-FACTOR; STEWARTII SUBSP STEWARTII; ROT ERWINIA BACTERIA; PSEUDOMONAS-SYRINGAE; PECTATE LYASE; NUCLEOTIDE-SEQUENCE; UNITED-STATES; GENES; HRP; EXPRESSION AB Pectobacterium species are enterobacterial plant-pathogens that cause soft rot disease in diverse plant species. Unlike hemi-biotrophic plant pathogenic bacteria, the type III secretion system (T3SS) of Pectobacterium carotovorum subsp. carotovorum (P. carotovorum) appears to secrete only one effector protein, DspE. Previously, we found that the T3SS regulator HrpL and the effector DspE are required for P. carotovorum pathogenesis on leaves. Here, we identified genes up-regulated by HrpL, visualized expression of dspE in leaves, and established that DspE causes host cell death. DspE required its full length and WxxxE-like motifs, which are characteristic of the AvrE-family effectors, for host cell death. We also examined expression in plant leaves and showed that hrpL is required for the expression of dspE and hrpN, and that the loss of a functional T3SS had unexpected effects on expression of other genes during leaf infection. These data support a model where P. carotovorum uses the T3SS early in leaf infection to initiate pathogenesis through elicitation of DspE-mediated host cell death. C1 [Hogan, Clifford S.; Willis, David K.; Charkowski, Amy O.] Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. [Mole, Beth M.; Grant, Sarah R.] Univ N Carolina, Dept Biol & Curriculum Mol Biol & Genet, Chapel Hill, NC USA. [Willis, David K.] USDA ARS, Vegetable Crops Res Unit, Madison, WI USA. RP Charkowski, AO (reprint author), Univ Wisconsin, Dept Plant Pathol, Madison, WI 53706 USA. EM acharkowski@wisc.edu FU National Science Foundation Environmental Genomics Program [0412599]; USDA-Hatch project the Wisconsin Potato and Vegetable Growers Association [WISO1637] FX This work was funded by National Science Foundation Environmental Genomics Program through award number 0412599, USDA-Hatch project award WISO1637 the Wisconsin Potato and Vegetable Growers Association. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 45 TC 7 Z9 7 U1 1 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD JUN 3 PY 2013 VL 8 IS 6 AR e65534 DI 10.1371/journal.pone.0065534 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 157CD UT WOS:000319872300086 PM 23755246 ER PT J AU Juneja, VK Altuntas, EG Ayhan, K Hwang, CA Sheen, S Friedman, M AF Juneja, Vijay K. Altuntas, Evrim Gunes Ayhan, Kamuran Hwang, Cheng-An Sheen, Shiowshuh Friedman, Mendel TI Predictive model for the reduction of heat resistance of Listeria monocytogenes in ground beef by the combined effect of sodium chloride and apple polyphenols SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Listeria monocytogenes; Ground beef; Sodium chloride; Apple polyphenols; Heat resistance; Microbial food safety ID SHIGA TOXIN 2; THERMAL INACTIVATION; ESCHERICHIA-COLI; STAPHYLOCOCCUS-AUREUS; SALMONELLA-ENTERICA; DESTRUCTION; TEMPERATURE; MEAT; PATHOGENS; EXTRACTS AB We investigated the combined effect of three internal temperatures (57.5, 60, and 62.5 degrees C) and different concentrations (0 to 3.0 wt/wt.%) of sodium chloride (NaCl) and apple polyphenols (APP), individually and in combination, on the heat-resistance of a five-strain cocktail of Listeria monocytogenes in ground beef. A complete factorial design (3 x 4 x 4) was used to assess the effects and interactions of heating temperature, NaCl, and APP. All 48 combinations were tested twice, to yield 96 survival curves. Mathematical models were then used to quantitate the combined effect of these parameters on heat resistance of the pathogen. The theoretical analysis shows that compared with heat alone, the addition of NaCl enhanced and that of APP reduced the heat resistance of L. monocytogenes measured as D-values. By contrast, the protective effect of NaCl against thermal inactivation of the pathogen was reduced when both additives were present in combination, as evidenced by reduction of up to similar to 68% in D-values at 57.5 degrees C; 65% at 60 degrees C; and 25% at 62.5 degrees C. The observed high antimicrobial activity of the combination of APP and low salt levels (e.g., 2.5% APP and 0.5% salt) suggests that commercial and home processors of meat could reduce the salt concentration by adding APP to the ground meat The influence of the combined effect allows a reduction of the temperature of heat treatments as well as the salt content of the meat Meat processors can use the predictive model to design processing times and temperatures that can protect against adverse effects of contaminated meat products. Additional benefits include reduced energy use in cooking, and the addition of antioxidative apple polyphenols may provide beneficial health affects to consumers. Published by Elsevier B.V. C1 [Juneja, Vijay K.; Hwang, Cheng-An; Sheen, Shiowshuh] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Altuntas, Evrim Gunes; Ayhan, Kamuran] Ankara Univ, Fac Engn, Dept Food Engn, TR-06100 Ankara, Turkey. [Friedman, Mendel] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Juneja, VK (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Vijay.Juneja@ars.usda.gov RI GUNES ALTUNTAS, EVRIM/O-1666-2014; OI GUNES ALTUNTAS, EVRIM/0000-0003-4897-9388; Friedman, Mendel/0000-0003-2582-7517 NR 45 TC 12 Z9 13 U1 5 U2 49 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD JUN 3 PY 2013 VL 164 IS 1 BP 54 EP 59 DI 10.1016/j.ijfoodmicro.2013.03.008 PG 6 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA 152PF UT WOS:000319542900009 PM 23587714 ER PT J AU Fernandez-Trujillo, JP Lester, GE Dos-Santos, N Martinez, JA Esteva, J Jifon, JL Varo, P AF Pablo Fernandez-Trujillo, Juan Lester, Gene E. Dos-Santos, Noelia Antonio Martinez, Juan Esteva, Juan Jifon, John L. Varo, Placido TI Pre- and Postharvest Muskmelon Fruit Cracking: Causes and Potential Remedies SO HORTTECHNOLOGY LA English DT Review DE Cucumis melo; environmental factors; fruit splitting; fruit quality; near-isogenic lines; netting; physiological disorder ID NEAR-ISOGENIC LINES; MELO VAR. MOMORDICA; CUCUMIS-MELO; SALMONELLA-POONA; CANTALOUPE RIND; QUALITY; WATER; RESISTANCE; PHYSIOLOGY; DISORDERS AB Fruit cracking is an important disorder that can cause severe loss of marketable yield and revenue in the muskmelon (Cucumis ludo) fruit industry. The physiological and environmental factors causing cracking are poorly understood. Although generally considered a physiological disorder caused by fluctuating environmental conditions, current evidence indicates that this disorder also has a genetic as well as a genotype x environment component. Certain cultivars are more susceptible than others, but wide fluctuations in irrigation, temperature, and nutrition during late fruit maturation stages appear to predispose fruit to cracking. This article summarizes the current state of our understanding of the causes of fruit splitting in muskmelons. C1 [Pablo Fernandez-Trujillo, Juan; Dos-Santos, Noelia] Tech Univ Cartagena UPCT, Dept Agr & Food Engn, ETSIA, Cartagena 30203, Murcia, Spain. [Pablo Fernandez-Trujillo, Juan; Dos-Santos, Noelia; Antonio Martinez, Juan; Esteva, Juan] Inst Plant Biotechnol, Cartagena 30203, Murcia, Spain. [Lester, Gene E.] ARS, USDA, Food Qual Lab, BARC West, Beltsville, MD 20705 USA. [Antonio Martinez, Juan; Esteva, Juan] Tech Univ Cartagena UPCT, Dept Plant Prod, ETSIA, Cartagena 30203, Murcia, Spain. [Jifon, John L.] Texas A&M Univ Syst, Texas A&M AgriLife Res & Extens Ctr, Weslaco, TX 78596 USA. [Varo, Placido] Consejeria Agr & Agua Reg Murcia, CIFEA, Torre Pacheco 30700, Murcia, Spain. RP Fernandez-Trujillo, JP (reprint author), Tech Univ Cartagena UPCT, Dept Agr & Food Engn, ETSIA, Paseo Alfonso 13,48, Cartagena 30203, Murcia, Spain. EM juanp.fdez@upct.es RI Fernandez Trujillo, Juan Pablo/I-5859-2013; Martinez, Juan Antonio/E-5492-2017 OI Fernandez Trujillo, Juan Pablo/0000-0002-5296-0308; Martinez, Juan Antonio/0000-0001-5712-1071 FU Fundacion Seneca de la Region de Murcia [11784/PI/09, 05676/PI/07]; Ministry of Innovation and Science; UE FEDER funds [AGL2010-20858, AGL2003-09175-C02-02]; USDA-ARS National Program [306]; USDA-ARS [1245-43440-004-00D] FX This work was supported by Fundacion Seneca de la Region de Murcia, (projects 11784/PI/09 and 05676/PI/07), Ministry of Innovation and Science and UE FEDER funds (AGL2010-20858, AGL2003-09175-C02-02). This study supports USDA-ARS National Program 306 and was partially funded by USDA-ARS Project 1245-43440-004-00D. Thanks to IRTA-CRAG for providing the seeds of the NILs and to A.J. Monforte (IBMCP "Eduardo Primo Yufera"-UPV-CSIC, Valencia, Spain) for valuable comments. We acknowledge the assistance of the team of CIFEA-Torre Pacheco and Estacion Experimental Agroalimentaria "Toms Ferro" (La Palma, Cartagena) for crop management, and to Javier M. Obando-Ulloa, Claudia Miranda, Ana B. Perez and M. Mandi Jowkar for technical assistance. NR 70 TC 2 Z9 2 U1 1 U2 11 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD JUN PY 2013 VL 23 IS 3 BP 266 EP 275 PG 10 WC Horticulture SC Agriculture GA AD7XM UT WOS:000333480800001 ER PT J AU Hahn, DC Summers, SG Genovese, KJ He, HQ Kogut, MH AF Hahn, D. Caldwell Summers, Scott G. Genovese, Kenneth J. He, Haiqi Kogut, Michael H. TI Enhanced Innate Immune Responses in a Brood Parasitic Cowbird Species: Degranulation and Oxidative Burst SO AVIAN DISEASES LA English DT Article DE innate immunity; oxidative burst; degranulation; eco-immunology; cowbird; brood parasite; West Nile virus ID WEST-NILE-VIRUS; COMMERCIAL BROILER-CHICKENS; IN-VITRO; EXPERIMENTAL-INFECTION; LIFE-HISTORY; BIRDS; HETEROPHILS; TRANSMISSION; ENTERITIDIS; POPULATION AB We examined the relative effectiveness of two innate immune responses in two species of New World blackbirds (Passeriformes, Icteridae) that differ in resistance to West Nile virus (WNV). We measured degranulation and oxidative burst, two fundamental components of phagocytosis, and we predicted that the functional effectiveness of these innate immune responses would correspond to the species' relative resistance to WNV. The brown-headed cowbird (Molothrus ater), an obligate brood parasite, had previously shown greater resistance to infection with WNV, lower viremia and faster recovery when infected, and lower subsequent antibody titers than the red-winged blackbird (Agelaius phoeniceus), a close relative that is not a brood parasite. We found that cowbird leukocytes were significantly more functionally efficient than those of the blackbird leukocytes and 50% more effective at killing the challenge bacteria. These results suggest that further examination of innate immunity in the cowbird may provide insight into adaptations that underlie its greater resistance to WNV. These results support an eco-immunological interpretation that species like the cowbird, which inhabit ecological niches with heightened exposure to parasites, experience evolutionary selection for more effective immune responses. C1 [Hahn, D. Caldwell] US Geol Survey, Patuxent Wildlife Res Ctr, Laurel, MD 20708 USA. [Summers, Scott G.] Nature Conservancy, Killeen, TX 76544 USA. [Genovese, Kenneth J.; He, Haiqi; Kogut, Michael H.] ARS, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. RP Hahn, DC (reprint author), US Geol Survey, Patuxent Wildlife Res Ctr, 12100 Beech Forest Rd, Laurel, MD 20708 USA. EM chahn@usgs.gov FU United States Geological Survey-Patuxent Wildlife Research Center; USDA-ARS-Southern Plains Agricultural Research Center FX This work was supported by United States Geological Survey-Patuxent Wildlife Research Center and by USDA-ARS-Southern Plains Agricultural Research Center. Use of trade, product, or firm names does not imply endorsement by the U.S. Government. We thank The Nature Conservancy of Texas and Fort Hood for assistance. We appreciate the comments of B. E. Kus, K. D. Lafferty, and two anonymous reviewers, which improved the manuscript. NR 44 TC 0 Z9 0 U1 1 U2 7 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 BP 285 EP 289 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AD4CA UT WOS:000333192600019 PM 24689187 ER PT J AU Register, KB AF Register, Karen B. TI Development of a PCR for Identification of Bordetella hinzii SO AVIAN DISEASES LA English DT Article DE Bordetella hinzii; ompA; PCR ID RESTRICTION ENZYME ANALYSIS; SP-NOV; AVIUM; DIFFERENTIATION; PREVALENCE; BACTERIA; POULTRY; GENE AB Bordetella hinzii infects primarily poultry and immunocompromised humans. It is closely related to the etiologic agent of turkey coryza, Bordetella avium. Distinguishing between B. avium and B. hinzii is difficult, and there is no method for identification of B. hinzii suitable for use by diagnostic laboratories. This report details the development of a B. hinzii-specific PCR targeting the ompA gene. Assay sensitivity is 100% based on analysis of 48 B. hinzii isolates from diverse geographic locations representing all known ribotypes. Evaluation of 71 isolates of B. avium and 20 other bacterial isolates from poultry, comprising gram-negative and gram-positive commensals and pathogens of nine genera, demonstrated an assay specificity of 100%. The ompA PCR is a rapid, reliable, and accurate method for identification of B. hinzii and provides a valuable new tool for veterinary diagnostic laboratories investigating poultry respiratory disease outbreaks. C1 ARS, Ruminant Dis & Immunol Res Unit, USDA, Natl Anim Dis Ctr, Ames, IA 50010 USA. RP Register, KB (reprint author), ARS, Ruminant Dis & Immunol Res Unit, USDA, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM karen.register@ars.usda.gov NR 16 TC 1 Z9 1 U1 1 U2 7 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 BP 307 EP 310 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA AD4CA UT WOS:000333192600024 PM 24689192 ER PT J AU Pedersen, JC Hines, NL Killian, ML Predgen, AS Schmitt, BJ AF Pedersen, Janice C. Hines, Nichole L. Killian, Mary Lea Predgen, Ann S. Schmitt, Beverly J. TI Isolation of a Virulent Newcastle Disease Virus from Confiscated LaSota Vaccine SO AVIAN DISEASES LA English DT Article DE Newcastle disease; real-time RT-PCR; vaccine; virulent Newcastle disease ID CANCER; PATHOGENESIS; CALIFORNIA; CHICKENS; OUTBREAK; THERAPY; BIRDS AB Vials of Newcastle disease vaccine labeled as LaSota were confiscated by the Arizona Division of Customs and Border Protection officials. Two different avian type 1 paramyxoviruses were isolated from all three vials of vaccine submitted to the National Veterinary Services Laboratories. The LaSota strain of avian paramyxovirus type 1 virus was isolated from all three vials and analyzed by nucleotide sequence analysis. A virulent Newcastle disease virus was also present in all three vials, but in low concentration. The virulence of the Newcastle disease virus was characterized by the intracerebral chicken pathogenicity index chicken inoculation assay but could not be determined by nucleotide sequence analysis from the virus isolated from embryonating chicken eggs. The intracerebral chicken pathogenicity index value for the isolated Newcastle disease virus was 1.55. Strains of Newcastle disease virus with intracerebral pathogenicity indexes significantly above 1.0 have been found to selectively kill many types of cancer cells while not affecting normal nonneoplastic cells and are considered to be a viable option for cancer treatment in humans by alternative medical researchers; however, the treatment is not approved for use in the United States by the Food and Drug Administration. Customs and Border Protection officials have been notified of an increased risk of Newcastle disease virus entering the United States for use as a nonapproved cancer treatment. Illegal importation of Newcastle disease vaccine for vaccination of backyard poultry is also a threat. This case report emphasizes the importance of conducting chicken inoculation for complete virus pathotyping and demonstrates the need for stringent security procedures at U.S. borders to detect known livestock pathogens that may be smuggled in for use in animal agriculture and reasons unrelated to animal agriculture. C1 [Pedersen, Janice C.; Hines, Nichole L.; Killian, Mary Lea; Schmitt, Beverly J.] US Anim & Plant Hlth Inspect Serv, Diagnost Virol Lab, Natl Vet Serv Labs, Vet Serv,USDA, Ames, IA 50010 USA. [Predgen, Ann S.] US Anim & Plant Hlth Inspect Serv, Pathobiol Lab, Natl Vet Serv Labs, Vet Serv,USDA, Ames, IA 50010 USA. RP Pedersen, JC (reprint author), US Anim & Plant Hlth Inspect Serv, Diagnost Virol Lab, Natl Vet Serv Labs, Vet Serv,USDA, Ames, IA 50010 USA. EM Janice.c.pedersen@aphis.usda.gov NR 18 TC 0 Z9 0 U1 1 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 BP 316 EP 320 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AD4CA UT WOS:000333192600026 PM 24689194 ER PT J AU Luo, J Chang, S Zhang, HM Li, BC Song, JZ AF Luo, Juan Chang, Shuang Zhang, Huanmin Li, Bichun Song, Jiuzhou TI DNA Methylation Down-Regulates EGFR Expression in Chickens SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE EGFR; promoter; methylation; Marek's disease virus; chicken ID EPIDERMAL-GROWTH-FACTOR; EPSTEIN-BARR-VIRUS; FACTOR RECEPTOR; BINDING; CELLS; PROLIFERATION; THERAPY; CANCER; LINES; GENE AB The epidermal growth factor receptor (EGFR), a growth-factor-receptor tyrosine kinase, is up-regulated in numerous tumors, which provides a good target for cancer therapy. Although it has been documented that oncoviruses are responsible for the activation of EGFR in tumors, the impact of Marek's disease virus (MDV) infection on EGFR has not yet been studied. We performed quantitative reverse transcriptase (RT)-PCR to check EGFR expression and found that it was significantly down-regulated after MDV infection. To explore the mechanism of EGFR repression, we examined the level of methylation of the EGFR promoter. The methylation level was significantly increased at 21 days postinfection, indicating a potential role of promoter methylation in EGFR repression. C1 [Luo, Juan; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Chang, Shuang; Zhang, Huanmin] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Chang, Shuang] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Li, Bichun] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Peoples R China. RP Song, JZ (reprint author), Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. EM songj88@umd.edu FU USDA-NRI/NIFA [2008-35204-04660, 2010-65205-20588] FX We thank Dr. Tom Porter for providing the pRL-SV40 Vector, Dr. Xiaoping Zhu for providing the HeLa cell line, and Dr. Mary E. Delany for providing the DF1 cell line. The work was supported by USDA-NRI/NIFA 2008-35204-04660 and USDA-NRI/NIFA 2010-65205-20588. NR 29 TC 1 Z9 1 U1 2 U2 6 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 366 EP 371 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700008 PM 23901748 ER PT J AU Volkening, JD Spatz, SJ AF Volkening, Jeremy D. Spatz, Stephen J. TI Identification and Characterization of the Genomic Termini and Cleavage/Packaging Signals of Gallid Herpesvirus Type 2 SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease; gallid herpesvirus 2; cleavage; packaging; termini ID MAREKS-DISEASE VIRUS; MULTIPLE SEQUENCE ALIGNMENT; INVERTED REPEAT REGIONS; A-SEQUENCE; DNA-SEQUENCE; CLEAVAGE; ACCURACY; STRAIN; PATHOTYPES; MUTATIONS AB Herpesvirus replication within host cells results in concatemeric genomic DNA, which is cleaved into unit-length genomes and packaged into the capsid by a complex of proteins. The sites of cleavage have been identified for many herpesviruses, and conserved signaling sequences involved in cleavage and packaging have been characterized. The cleavage/packaging motifs pac-1, pac-2, and DR1 and two distinct groups of telomeric repeat sequences (static TRS and variable TRS) have been identified. By sequencing the termini of the gallid herpesvirus type 2 (GaHV-2) strain CU-2, two different cleavage sites (classical and aberrant) have been identified. Unlike classical cleavage of human herpesvirus type 1, which occurs within the DR1 site, classical cleavage of the GaHV-2 concatemers occurs 8.5 bp upstream of the DR1 site and results in an S-terminus containing telomeric repeats. Aberrant cleavage occurs the same distance from the DR1 site and generates a telomeric S-terminus but an L-terminus lacking an a sequence. These results are consistent with previous findings in other herpesviruses and should prove useful in the future study and manipulation of the GaHV-2 genome. C1 [Volkening, Jeremy D.] BASE2BIO, Madison, WI 53714 USA. [Spatz, Stephen J.] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Spatz, SJ (reprint author), ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. EM stephen.spatz@ars.usda.gov OI Volkening, Jeremy/0000-0002-8892-7155 FU U.S. Department of Agriculture, CRIS [6612-32000-052, 6612-32000-065] FX This research was funded by the U.S. Department of Agriculture, CRIS numbers 6612-32000-052 and 6612-32000-065. NR 39 TC 3 Z9 3 U1 0 U2 1 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 401 EP 408 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700013 PM 23901753 ER PT J AU Lupiani, B Lee, LF Kreager, KS Witter, RL Reddy, SM AF Lupiani, Blanca Lee, Lucy F. Kreager, K. S. Witter, Richard L. Reddy, Sanjay M. TI Insertion of Reticuloendotheliosis Virus Long Terminal Repeat into the Genome of CVI988 Strain of Marek's Disease Virus Results in Enhanced Growth and Protection SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease virus; CVI988; vaccine; pathogenesis; protection; LTR ID VIRULENT PLUS STRAIN; CONFERS PROTECTION; FIELD STRAIN; IN-VIVO; MUTANT; CHALLENGE; REPLICATION; HERPESVIRUS; VACCINE; GENE AB Marek's disease (MD) is a lymphoproliferative disease of chickens caused by serotype 1 MD virus (MDV). Vaccination of commercial poultry has drastically reduced losses from MD, and the poultry industry cannot be sustained without the use of vaccines. Retrovirus insertion into herpesvirus genomes is an efficient process that alters the biological properties of herpesviruses. RM1, a virus derived from the virulent JM strain of MDV, by insertion of the reticuloendotheliosis (REV) long terminal repeat (LTR), was attenuated for oncogenicity but retains properties of the parental virus, such as lymphoid organ atrophy. Here we show that insertion of the REV LTR into the genome of vaccine strain CVI988 resulted in a virus (CVRM) that replicated to higher levels than parental CVI988 in cell culture and that remained apathogenic for chickens. In addition, CVRM showed protection indices similar or superior to those afforded by CVI988 virus in laboratory and field protection trials, indicating that it could be developed as a safe and efficacious vaccine to protect against very virulent plus MDV. C1 [Lupiani, Blanca; Reddy, Sanjay M.] Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Lee, Lucy F.; Witter, Richard L.] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. [Kreager, K. S.] Hy Line Int, Dallas Ctr, IA 50063 USA. RP Reddy, SM (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. EM sreddy@cvm.tamu.edu FU U.S. Department of Agriculture Formula Animal Health [TEX09530]; NIFA/AFRI/USDA [2010-65119-20547] FX We thank Mr. Barry Coulson for his excellent technical assistance. This work was initiated at the Avian Disease and Oncology Laboratory, ARS, USDA, East Lansing, MI. This project was partially supported by U.S. Department of Agriculture Formula Animal Health grant number TEX09530, awarded to B. L., and NIFA/AFRI/USDA grant number 2010-65119-20547, awarded to S.M.R. and B.L. NR 13 TC 4 Z9 7 U1 0 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 427 EP 431 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700016 PM 23901756 ER PT J AU Sun, AJ Lee, LF Khan, OA Heidari, M Zhang, HM Lupiani, B Reddy, SM AF Sun, Aijun Lee, Lucy F. Khan, Owais A. Heidari, Mohammad Zhang, Huanmin Lupiani, Blanca Reddy, Sanjay M. TI Deletion of Marek's Disease Virus Large Subunit of Ribonucleotide Reductase Impairs Virus Growth In Vitro and In Vivo SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease virus; bacterial artificial chromosome; ribonucleotide reductase; UL39; Md5 strain; pathogenesis ID BACTERIAL ARTIFICIAL CHROMOSOME; MUTANT; HERPESVIRUS; GENE; SEQUENCE; CULTURE; GENOME; STRAIN AB Marek's disease virus (MDV), a highly cell-associated lymphotropic alphaherpesvirus, is the causative agent of a neoplastic disease in domestic chickens called Marek's disease (MD). In the unique long (UL) region of the MDV genome, open reading frames UL39 and UL40 encode the large and small subunits of the ribonucleotide reductase (RR) enzyme, named RR1 and RR2, respectively. MDV RR is distinguishable from that present in chicken and duck cells by monoclonal antibody T81. Using recombinant DNA technology we have generated a mutant MDV (Md5 Delta RR1) in which RR1 was deleted. PCR amplification of the RR gene in Md5 Delta RR1-infected duck embryo fibroblasts (DEF) confirmed the deletion of the 2.4 kb RR1 gene with a resultant amplicon of a 640-bp fragment. Restriction enzyme digests with SalI confirmed a UL39 deletion and the absence of gross rearrangement. The biologic characteristics of Md5 Delta RR1 virus were studied in vitro and in vivo. The Md5 Delta RR1 replicated in DEF, but significantly slower than parental Md5-BAC, suggesting that RR is important but not essential for replication in fibroblasts. In vivo studies, however, showed that the RR1 deletion virus was impaired for its ability to replicate in chickens. Inoculation of specific-pathogen-free (SPF) chickens with Md5 Delta RR1 showed the mutant virus is nonpathogenic and does not induce MD in birds. A revertant virus, Md5 Delta RR1/R, was generated with the restored phenotype of the parental Md5-BAC in vivo, indicating that RR is essential for replication of the virus in chickens. Protection studies in SPF chickens indicated that the Md5 Delta RR1 virus is not a candidate vaccine against MD. C1 [Sun, Aijun; Khan, Owais A.; Lupiani, Blanca; Reddy, Sanjay M.] Texas A&M Univ, Dept Vet Pathobiol, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Lee, Lucy F.; Heidari, Mohammad; Zhang, Huanmin] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. RP Reddy, SM (reprint author), Texas A&M Univ, Dept Vet Pathobiol, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. EM sreddy@cvm.tamu.edu FU United States Department of Agriculture Formula Animal Health [TEX09528]; NIFA/AFRI/USDA [2010-65119-20547] FX We would like to thank Dr. Hans Cheng for providing the BAC clone of Md5 and Dr. Klaus Osterrieder for providing reagents to perform two-step red-mediated recombination. We thank Mr. Barry Coulson for his excellent technical assistance. This project was partially supported by the United States Department of Agriculture Formula Animal Health TEX09528 awarded to S. M. R. and NIFA/AFRI/USDA grant number 2010-65119-20547 awarded to S.M.R. and B.L. NR 31 TC 3 Z9 3 U1 0 U2 2 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 464 EP 468 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700022 PM 23901762 ER PT J AU Reddy, SM Sun, AJ Khan, OA Lee, LF Lupiani, B AF Reddy, Sanjay M. Sun, Aijun Khan, Owais A. Lee, Lucy F. Lupiani, Blanca TI Cloning of a Very Virulent Plus, 686 Strain of Marek's Disease Virus as a Bacterial Artificial Chromosome SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease virus; bacterial artificial chromosome; very virulent plus; vv; 686 strain; pathogenesis ID CULTURE; MUTANT; GENOME; GENE AB Bacterial artificial chromosome (BAC) vectors were first developed to facilitate propagation and manipulation of large DNA fragments. This technology was later used to clone full-length genomes of large DNA viruses to study viral gene function. Marek's disease virus (MDV) is a highly oncogenic herpesvirus that causes rapid induction of T-cell lymphomas in chickens. Based on the virus's ability to cause disease in vaccinated chickens, MDV strains are classified into pathotypes, with the most virulent strains belonging to the very virulent plus (vv+) pathotype. Here we report the construction of BAC clones of 686 (686-BAC), a vv+ strain of MDV. Transfection of DNA isolated from two independent clones into duck embryo fibroblasts resulted in recovery of infectious virus. Pathogenesis studies showed that the BAC-derived 686 viruses were more virulent than Md5, a vv strain of MDV. With the use of a two-step red-mediated mutagenesis process, both copies of viral interleukin 8 (vIL-8) were deleted from the MDV genome, showing that 686-BACs were amenable to mutagenesis techniques. The generation of BAC clones from a vv+ strain of MDV is a significant step toward understanding molecular basis of MDV pathogenesis. C1 [Reddy, Sanjay M.; Sun, Aijun; Khan, Owais A.; Lupiani, Blanca] Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. [Lee, Lucy F.] ARS, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. RP Lupiani, B (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. EM blupiani@cvm.tamu.edu FU NIFA/AFRI/USDA [2010-65119-20547] FX We would like to thank Dr. Klaus Osterrieder for providing BAC reagents necessary for cloning and mutagenesis. We are grateful to Dr. Richard Witter for providing DNA from 686 strain of MDV and Dr. Hans Cheng for BAC clone used to generate Md5-BAC virus. This work is supported by NIFA/AFRI/USDA grant 2010-65119-20547 awarded to SMR and BL. NR 19 TC 0 Z9 1 U1 0 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 469 EP 473 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700023 PM 23901763 ER PT J AU Hunt, HD Dunn, JR AF Hunt, Henry D. Dunn, John R. TI The Influence of Host Genetics on Marek's Disease Virus Evolution SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease; virus; virulence; evolution; major histocompatibility complex ID GALLID-HERPESVIRUS TYPE-2; T-LYMPHOCYTE RESPONSE; STRAIN CVI 988; CHICKEN LINES; GLYCOPROTEIN L; FIELD TRIALS; B-HAPLOTYPE; IN-VITRO; HISTOCOMPATIBILITY; RESISTANCE AB Since the first report of a polyneuritis in chickens by Joseph Marek in 1907, the clinical nature of the disease has changed. Over the last five decades, the pathogenicity of the Marek's disease virus (MDV) has continued to evolve from the relatively mild strains observed in the 1960s to the more severe strains labeled very virulent plus currently observed in today's outbreaks. To understand the influence of host genetics, specifically the major histocompatibility complex (MHC), on virus evolution, a bacterial artificial chromosome-derived MDV (Md5B40BAC) was passed in vivo through resistant (MHC-B21) and susceptible (MHC-B13) Line 0 chickens. Criteria for selecting virus isolates for in vivo passage were based on virus replication in white blood cells 21 days after challenge and evaluation of MD pathology at necropsy. In the MHC-B13-susceptible line the Md5B40BAC virulence consistently increased from 18% Marek's disease (MD) after in vivo passage 1 (B13-IVP1 Md5B40BAC) to 94% MD after B13-IVP5 Md5B40BAC challenge. In the MHC-B21-resistant line MD virulence fluctuated from 28% at B21-IVP1 Md5B40BAC to a high of 65% in B21-IVP2 Md5B40BAC back to a low of 23% in B21-IVP5 Md5B40BAC-challenged chicks. Although the B21-IVP5 Md5B40BAC isolates were relatively mild in the MHC-B21 chicken line (56% MDV), they were highly virulent in the MHC-B13 line (100% MDV). From this series of experiments it would appear that MDV evolution toward greater virulence occurs in both susceptible and resistant MHC haplotypes, but the resulting increase in pathogenicity is constrained by the resistant MHC haplotype. C1 [Hunt, Henry D.; Dunn, John R.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Hunt, HD (reprint author), ARS, USDA, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM Henry.Hunt@ars.usda.gov NR 52 TC 8 Z9 8 U1 1 U2 5 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 474 EP 482 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700024 PM 23901764 ER PT J AU Dunn, JR Gimeno, IM AF Dunn, John R. Gimeno, Isabel M. TI Current Status of Marek's Disease in the United States and Worldwide Based on a Questionnaire Survey SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease; MDV; poultry; epidemiology; incidence; questionnaire ID TURKEY HERPESVIRUS VACCINE; STRAIN CVI 988; VIRUS; CHICKENS; PROTECTION; NETHERLANDS; VIRULENCE; TRIALS AB A questionnaire was widely distributed in 2011 to estimate the global prevalence of Marek's disease (MD) and gain a better understanding of current control strategies and future concerns. A total of 112 questionnaires were returned representing 116 countries from sources including national branch secretaries of the World Veterinary Poultry Association, vaccine, breeder, and production companies, as well as MD researchers from various backgrounds. Each country listed on a questionnaire was recorded as an individual entry, and on average there were 2.0 entries per country (median 1; range 1-13). All flock types were listed as having increased MD incidence during the last 10 yr in close to 50% of countries by at least one respondent, with the majority of these countries located within French-speaking Africa, Eastern Europe, East Asia, and South America. Only 18 countries (16%) indicated increasing MD incidence was likely due to higher virulent strains, while the presence of other immunosuppressive diseases was a more common explanation. Increased use of CVI988/Rispens was cited as the most likely reason for decreasing MD incidence in 49 countries (42%). In the United States, MD incidence has continued to decrease during the last 10 yr, reaching a record low in 2007 (0.0008%) as measured by leukosis condemnation rates in broilers at slaughter. However, a recent increase of leukosis condemnations in North Carolina and Pennsylvania needs to be closely monitored. C1 [Dunn, John R.] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Gimeno, Isabel M.] N Carolina State Univ, Dept Populat Hlth & Pathobiol, Coll Vet Med, Raleigh, NC 27606 USA. RP Dunn, JR (reprint author), ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. EM john.dunn@ars.usda.gov NR 29 TC 9 Z9 9 U1 0 U2 4 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 483 EP 490 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700025 PM 23901765 ER PT J AU Lee, LF Kreager, K Heidari, M Zhang, HM Lupiani, B Reddy, SM Fadly, A AF Lee, Lucy F. Kreager, Kenton Heidari, Mohammad Zhang, Huanmin Lupiani, Blanca Reddy, Sanjay M. Fadly, Aly TI Properties of a meq-Deleted rMd5 Marek's Disease Vaccine: Protection Against Virulent MDV Challenge and Induction of Lymphoid Organ Atrophy Are Simultaneously Attenuated by Serial Passage In Vitro SO AVIAN DISEASES LA English DT Article; Proceedings Paper CT 9th International Symposium on Marek's Disease and Avian Herpesviruse CY JUN 24-28, 2012 CL Berlin, GERMANY DE Marek's disease virus; Meq null virus; rMd5 Delta Meq; vaccine; chicken ID STRAIN CVI 988; INSERTIONAL MUTAGENESIS; MATERNAL ANTIBODIES; VIRUS; HERPESVIRUS; REPLICATION; EFFICACY; MUTANT; GENE; TRANSFORMATION AB We have previously shown that deletion of the meq gene from the genome of Cosmid-cloned rMd5 strain of Marek's disease virus (MDV-1) resulted in loss of transformation and oncogenic capacity of the virus. The rMd5 Delta Meq (Meq null) virus has been shown to be an excellent vaccine in maternal antibody positive (MAb+) chickens challenged with a very virulent plus (vv+) strain of MDV, 648A. The only drawback was that it retained its ability to induce bursa and thymus atrophy (BTA) like that of the parental rMd5 in maternal antibody negative (MAb-) chickens. We recently reported that the attenuated Meq null virus did not induce BTA at the 40th cell culture passage onward. Its protective ability against challenge with vv+ MDV, strain 686 was similar to the original virus at the 19th passage in MAb- chickens. In this study, we compared the same series of attenuated meq null viruses in commercial chickens. In commercial chickens with MAb, the attenuated viruses quickly lost protection with increasing cell culture attenuation. These data suggest that although attenuation of these meq null viruses eliminated BTA, it had no influence on their protective efficacy in MAb- chickens. However, in commercial chickens (MAb+), the best protection was provided by the original 19th passage; the attenuated 40th passage was as good as one of the currently commercial CVI988/Rispens vaccine, and it did not induce BTA. Therefore, protection against virulent MDV challenge and induction of lymphoid organ atrophy are simultaneously attenuated by serial passage in vitro. C1 [Lee, Lucy F.; Heidari, Mohammad; Zhang, Huanmin; Fadly, Aly] ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Kreager, Kenton] Hy Line Int, Dallas Ctr, Dallas Ctr, IA 50063 USA. [Lupiani, Blanca; Reddy, Sanjay M.] Texas A&M Univ, Dept Vet Pathobiol, Coll Vet Med & Biomed Sci, College Stn, TX 77843 USA. RP Lee, LF (reprint author), ARS, USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. EM leelu@msu.edu NR 38 TC 4 Z9 5 U1 1 U2 3 PU AMER ASSOC AVIAN PATHOLOGISTS PI ATHENS PA 953 COLLEGE STATION RD, ATHENS, GA 30602-4875 USA SN 0005-2086 EI 1938-4351 J9 AVIAN DIS JI Avian Dis. PD JUN PY 2013 VL 57 IS 2 SU 1 BP 491 EP 497 PG 7 WC Veterinary Sciences SC Veterinary Sciences GA AD4DA UT WOS:000333195700026 PM 23901766 ER PT J AU Grant, N Saito, L Weltz, M Walker, M Daly, C Stewart, K Morris, C AF Grant, Nicholas Saito, Laurel Weltz, Mark Walker, Mark Daly, Christopher Stewart, Kelley Morris, Christo TI Instrumenting Wildlife Water Developments to Collect Hydrometeorological Data in Remote Western US Catchments SO JOURNAL OF ATMOSPHERIC AND OCEANIC TECHNOLOGY LA English DT Article ID UNITED-STATES; PRECIPITATION GAUGE; RAIN GAUGES; TEMPERATURE; ACCURACY; QUALITY; ARIZONA; TRENDS AB In the arid western United States, wildlife water developments, or "guzzlers,'' are important water sources for wildlife, and consist of impermeable roof structures designed to intercept precipitation and small tanks for storing water. Guzzlers are typically installed in remote mid-to high-elevation basins, where precipitation data are often scarce. In this study, small-game guzzlers were examined for feasibility as potential sites for improving estimates of climatic parameters in remote Nevada catchments. Instruments measuring liquid precipitation and water level were installed at two guzzler field sites. Although one field site was vandalized during the study, field results indicated that water levels in the tank measured by Hobo pressure transducers corresponded well with precipitation events measured by the Texas Electronics tipping-bucket rain gauge, and that measured data were similar to Parameter-Elevation Regressions on Independent Slopes Model (PRISM) estimates. Minimum temperatures from the guzzler sites were similar to PRISM; however, maximum temperatures were a few degrees higher, possibly because temperature sensors were unshielded. With over 1600 guzzlers in Nevada and thousands more throughout the western United States, this study initiates exploration of the feasibility of augmenting individual guzzler sites to enhance climatic monitoring at a relatively low cost to improve the quality and density of climate observations, benefitting hydrologists, climatologists, and wildlife managers. C1 [Grant, Nicholas; Saito, Laurel] Univ Nevada, Grad Program Hydrol Sci, Reno, NV 89557 USA. [Saito, Laurel; Walker, Mark; Stewart, Kelley] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Weltz, Mark; Morris, Christo] ARS, USDA, Reno, NV USA. [Daly, Christopher] Oregon State Univ, Sch Chem Biol & Environm Engn, PRISM Climate Grp, Northwest Alliance Computat Sci & Engn, Corvallis, OR 97331 USA. RP Saito, L (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Mail Stop 186, Reno, NV 89557 USA. EM lsaito@cabnr.unr.edu RI Saito, Laurel/E-3096-2010 OI Saito, Laurel/0000-0003-3617-3133 FU Nevada Agricultural Experiment Station; Bureau of Land Management; United States Department of Agriculture/Agriculture Research Service; Nevada Water Resources Association; University of Nevada/Reno Academy for the Environment FX Assistance with experimental trials, site instrumentation, and downloading was provided by Kate Grant, RobEngel (UNR), and Mike Potts (UNR). Assistance in the site selection process was provided by John Gebhardt (NDOW) and Clint Garret (NDOW). Materials for runoff collectors and assistance with collector design were provided by Wally Miller (UNR). Contributions to project planning were made by Jeff Underwood (UNR) and Kelly Redmond (WRCC). GIS assistance was provided by Jasmine Vittori (UNR). The authors thank Louis Provencher and Craig Stevenson and three anonymous reviewers for providing helpful comments on the manuscript. USDA is an equal opportunity provider and employer. Research was funded in part by the Nevada Agricultural Experiment Station, the Bureau of Land Management, the United States Department of Agriculture/Agriculture Research Service, the Nevada Water Resources Association, and the University of Nevada/Reno Academy for the Environment. Mention of a proprietary product does not constitute a guarantee or warranty of the product by USDA or the authors, and it does not imply its approval to the exclusion of the other products that also may be suitable. NR 25 TC 3 Z9 3 U1 2 U2 3 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 0739-0572 EI 1520-0426 J9 J ATMOS OCEAN TECH JI J. Atmos. Ocean. Technol. PD JUN PY 2013 VL 30 IS 6 BP 1161 EP 1170 DI 10.1175/JTECH-D-12-00065.1 PG 10 WC Engineering, Ocean; Meteorology & Atmospheric Sciences SC Engineering; Meteorology & Atmospheric Sciences GA 301EF UT WOS:000330514900010 ER PT J AU Blank, RR Morgan, T Clements, CD Mackey, BE AF Blank, Robert R. Morgan, Tye Clements, Charlie D. Mackey, Bruce E. TI Bromus tectorum L. Invasion: Changes in Soil Properties and Rates of Bioturbation SO SOIL SCIENCE LA English DT Article DE Bioturbation; cheatgrass; plant invasion ID NITROGEN AVAILABILITY; CALCAREOUS SOILS; PLANT INVASIONS; NATIVE GRASSES; CHEATGRASS; FIRE; COMMUNITIES; SEEDLINGS; DYNAMICS; DESERT AB Bromus tectorum (cheatgrass) has replaced native Artemisia (sagebrush)/bunchgrass communities throughout the Intermountain West. Using the framework of Jenny (1941), we hypothesized that invasion by B. tectorum could alter pedogenic trajectories. Hypothesis testing involved monitoring the invasion of a Krascheninnikovia lanata (winterfat) community by B. tectorum. In 1999, 13 points were established, 50 m apart, extending from the invasion front to noninvaded areas. For more than 11 years, soils were sampled randomly near each point, 0 to 20 cm, and analyzed for various soil attributes. Using autoregression, trends with time between invaded and noninvaded soils differed significantly (P <= 0.05) in solution-phase ortho-P and Ca+2, phosphatase activities, and net 30-day N mineralization potentials. Notable trends with time, regardless of invasion status, included a decline in solution-phase Mg+2 and K+ and an increase in soil pH and the molar proportion of NH4+ in the mineral N fraction. Relative to noninvaded soils, B. tectorum soils had greater mineral N and greater molar proportion of NH4+ in that fraction; greater amidase and phosphatase activities; greater solution-phase ortho-P and molar proportion of NO2- in the NO2--N + NO3--N pool; greater micronutrient availability of Fe and Cu; and higher soil pH. The decline in solution-phase Ca+2 and ortho-P and the increase in soil pH are likely caused by an invasion-fostered increase in bioturbation from rodents, which mixes carbonate-bearing soil from lower horizons to the soil surface. Changes in surface soil properties induced by both B. tectorum invasion and elevated bioturbation have potential long-term ramifications to soil pedogenesis and plant successional trajectories. C1 [Blank, Robert R.; Morgan, Tye; Clements, Charlie D.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV 89512 USA. [Mackey, Bruce E.] USDA ARS, Pacific West Area, Albany, CA USA. RP Blank, RR (reprint author), USDA ARS, Great Basin Rangelands Res Unit, 920 Valley Rd, Reno, NV 89512 USA. EM bob.blank@ars.usda.gov NR 57 TC 3 Z9 3 U1 5 U2 21 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD JUN PY 2013 VL 178 IS 6 BP 281 EP 290 DI 10.1097/SS.0b013e3182a4afd0 PG 10 WC Soil Science SC Agriculture GA 298JM UT WOS:000330320000002 ER PT J AU Kao, C Hsu, J Bandi, V Jahoor, F AF Kao, Christina Hsu, Jean Bandi, Venkata Jahoor, Farook TI Alterations in glutamine metabolism and its conversion to citrulline in sepsis SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE severe sepsis; stable isotopes; enterocyte ID SPLANCHNIC BED UTILIZATION; CRITICALLY-ILL PATIENTS; DE-NOVO SYNTHESIS; AMINO-ACID; WHOLE-BODY; HAND VEIN; HUMANS; ARGININE; KINETICS; LEUCINE AB In enterocytes, glutamine serves as the major source of energy; another metabolic fate of glutamine is conversion to citrulline. Because sepsis can affect gut function and integrity, alterations in glutamine metabolism may exist and lead to decreased citrulline production. This study aimed to investigate how sepsis affects glutamine metabolism, including its conversion to citrulline, by measuring glutamine and citrulline flux, fractional splanchnic extraction of glutamine and leucine, and the contribution of glutamine nitrogen to citrulline in septic patients and healthy controls. Eight patients with severe sepsis and 10 healthy controls were given primed, constant intravenous infusion of [H-2(2)] citrulline and sequential administration of intravenous and enteral [alpha-N-15] glutamine and [C-13] leucine in the postabsorptive state. The results showed that, compared with healthy controls, septic patients had a significantly lower whole body citrulline flux and plasma concentration, higher endogenous leucine flux, and higher glutamine clearance. Fractional splanchnic extraction of leucine was higher in septic patients than in controls, but fractional extraction of glutamine was not different. The majority of the N-15 label transferred from glutamine to citrulline was found at the alpha-position. These results demonstrate that lower glutamine plasma concentrations in sepsis were a result of increased glutamine clearance. Despite adequate splanchnic uptake of glutamine, there is decreased production of citrulline, suggesting a defect in the metabolic conversion of glutamine to citrulline, decreased uptake of glutamine by the enterocyte but increased uptake by the liver, and/or shunting of glutamine to other metabolic pathways. C1 [Kao, Christina; Bandi, Venkata] Baylor Coll Med, Dept Med, Sect Pulm Crit Care & Sleep Med, Houston, TX 77030 USA. [Kao, Christina; Hsu, Jean; Jahoor, Farook] Baylor Coll Med, ARS, USDA, Dept Pediat,Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Kao, C (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, 1100 Bates St,7th Floor, Houston, TX 77030 USA. EM Christina.Kao@bcm.edu FU National Institute of Diabetes and Digestive and Kidney Disease [1K23DK08115]; National Institutes of Health [M01-RR-00188]; US Department of Agriculture, Agricultural Research Service [58-6250-6001] FX This work was supported in part by the National Institute of Diabetes and Digestive and Kidney Disease (1K23DK08115). Work at the General Clinical Research Center was supported by the National Institutes of Health (M01-RR-00188). This research was also supported with federal funds from the US Department of Agriculture, Agricultural Research Service under Cooperative Agreement number 58-6250-6001. NR 42 TC 19 Z9 21 U1 0 U2 3 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD JUN PY 2013 VL 304 IS 12 BP E1359 EP E1364 DI 10.1152/ajpendo.00628.2012 PG 6 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA 284VT UT WOS:000329350000012 PM 23612995 ER PT J AU Tussing-Humphreys, L Thomson, JL Mayo, T Edmond, E AF Tussing-Humphreys, Lisa Thomson, Jessica L. Mayo, Tanyatta Edmond, Emanuel TI A Church-Based Diet and Physical Activity Intervention for Rural, Lower Mississippi Delta African American Adults: Delta Body and Soul Effectiveness Study, 2010-2011 SO PREVENTING CHRONIC DISEASE LA English DT Article ID HEALTH-PROMOTION; FOOD-INTAKE; TRIAL; COMMUNITY; PROGRAM; ACCESS; FRUIT AB Introduction Obesity, diabetes, and hypertension have reached epidemic levels in the largely rural Lower Mississippi Delta (LMD) region. We assessed the effectiveness of a 6-month, church-based diet and physical activity intervention, conducted during 2010 through 2011, for improving diet quality (measured by the Healthy Eating Index-2005) and increasing physical activity of African American adults in the LMD region. Methods We used a quasi-experimental design in which 8 self-selected eligible churches were assigned to intervention or control. Assessments included dietary, physical activity, anthropometric, and clinical measures. Statistical tests for group comparisons included chi(2), Fisher's exact, and McNemar's tests for categorical variables, and mixed-model regression analysis for continuous variables and modeling intervention effects. Results Retention rates were 85% (176 of 208) for control and 84% (163 of 195) for intervention churches. Diet quality components, including total fruit, total vegetables, and total quality improved significantly in both control (mean [standard deviation], 0.3 [1.8], 0.2 [1.1], and 3.4 [9.6], respectively) and intervention (o.6 [1.7], 0.3 [1.2], and 3.2 [9.7], respectively) groups, while significant increases in aerobic (22%) and strength/flexibility (24%) physical activity indicators were apparent in the intervention group only. Regression analysis indicated that intervention participation level and vehicle ownership were significant positive predictors of change for several diet quality components. Conclusion This church-based diet and physical activity intervention may be effective in improving diet quality and increasing physical activity of LMD African American adults. Components key to the success of such programs are participant engagement in educational sessions and vehicle access. C1 [Tussing-Humphreys, Lisa] Univ Illinois, Chicago, IL 60618 USA. [Tussing-Humphreys, Lisa; Thomson, Jessica L.; Mayo, Tanyatta] ARS, USDA, Baton Rouge, LA USA. [Edmond, Emanuel] Delta Hlth Alliance, Stoneville, MS USA. RP Tussing-Humphreys, L (reprint author), Univ Illinois, 1747 W Roosevelt Rd, Chicago, IL 60618 USA. EM ltussing@uic.edu FU US Department of Agriculture, Agricultural Research Service Project [64015100000100D]; US Department of Health and Human Services, Health Resources and Services Administration [6 U1FRH07411] FX We thank the original study investigators who developed the intervention components and selected the survey measures. We would also like to thank the entire Delta Body and Soul research team. We are particularly thankful to the church leaders, committee members, and participants of the Delta Body and Soul study for their participation and tremendous support. This research was supported by the US Department of Agriculture, Agricultural Research Service Project 64015100000100D and US Department of Health and Human Services, Health Resources and Services Administration grant no. 6 U1FRH07411. NR 26 TC 2 Z9 2 U1 2 U2 5 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD JUN PY 2013 VL 10 AR UNSP 120286 DI 10.5888/pcd10.120286 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA 285KD UT WOS:000329391900002 ER PT J AU Gerber, PJ Hristov, AN Henderson, B Makkar, H Oh, J Lee, C Meinen, R Montes, F Ott, T Firkins, J Rotz, A Dell, C Adesogan, AT Yang, WZ Tricarico, JM Kebreab, E Waghorn, G Dijkstra, J Oosting, S AF Gerber, P. J. Hristov, A. N. Henderson, B. Makkar, H. Oh, J. Lee, C. Meinen, R. Montes, F. Ott, T. Firkins, J. Rotz, A. Dell, C. Adesogan, A. T. Yang, W. Z. Tricarico, J. M. Kebreab, E. Waghorn, G. Dijkstra, J. Oosting, S. TI Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: a review SO ANIMAL LA English DT Article; Proceedings Paper CT 5th Greenhouse Gases and Animal Agriculture (GGAA) Conference CY JUN 23-26, 2013 CL Dublin, IRELAND SP Univ Coll Dublin, Teagasc DE greenhouse gases; climate change; animal production; animal feeding; manure management ID GREENHOUSE-GAS EMISSIONS; LACTATING DAIRY-COWS; DIETARY NITRATE SUPPLEMENTATION; PHASE COMPOST BIOFILTERS; CEREAL GRAIN DIET; NITRIFICATION INHIBITORS; REDUCE METHANE; PIG SLURRY; ANIMAL PERFORMANCE; MANAGEMENT OPTIONS AB Although livestock production accounts for a sizeable share of global greenhouse gas emissions, numerous technical options have been identified to mitigate these emissions. In this review, a subset of these options, which have proven to be effective, are discussed. These include measures to reduce CH4 emissions from enteric fermentation by ruminants, the largest single emission source from the global livestock sector, and for reducing CH4 and N2O emissions from manure. A unique feature of this review is the high level of attention given to interactions between mitigation options and productivity. Among the feed supplement options for lowering enteric emissions, dietary lipids, nitrates and ionophores are identified as the most effective. Forage quality, feed processing and precision feeding have the best prospects among the various available feed and feed management measures. With regard to manure, dietary measures that reduce the amount of N excreted (e.g. better matching of dietary protein to animal needs), shift N excretion from urine to faeces (e.g. tannin inclusion at low levels) and reduce the amount of fermentable organic matter excreted are recommended. Among the many end-of-pipe' measures available for manure management, approaches that capture and/or process CH4 emissions during storage (e.g. anaerobic digestion, biofiltration, composting), as well as subsurface injection of manure, are among the most encouraging options flagged in this section of the review. The importance of a multiple gas perspective is critical when assessing mitigation potentials, because most of the options reviewed show strong interactions among sources of greenhouse gas (GHG) emissions. The paper reviews current knowledge on potential pollution swapping, whereby the reduction of one GHG or emission source leads to unintended increases in another. C1 [Gerber, P. J.; Henderson, B.; Makkar, H.] Food & Agr Org United Nations, Agr & Consumer protect Dept, I-00153 Rome, Italy. [Hristov, A. N.; Oh, J.; Lee, C.; Meinen, R.; Ott, T.] Penn State Univ, Dept Anim Sci, University Pk, PA 16802 USA. [Montes, F.] Penn State Univ, Dept Plant Sci, University Pk, PA 16802 USA. [Firkins, J.] Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA. [Rotz, A.; Dell, C.] ARS, Dept Anim Sci, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Adesogan, A. T.] Univ Florida, Gainesville, FL 32608 USA. [Yang, W. Z.] Agr & Agri Food Canada, Lethbridge, AB T1J 4B1, Canada. [Tricarico, J. M.] Innovat Ctr US Dairy, Rosemont, IL 60018 USA. [Kebreab, E.] Univ Calif Davis, Dept Anim Sci, Davis, CA 95616 USA. [Waghorn, G.] DairyNZ, Hamilton 3240, New Zealand. [Dijkstra, J.; Oosting, S.] Wageningen Univ, Dept Anim Sci, NL-6700 AH Wageningen, Netherlands. RP Gerber, PJ (reprint author), Food & Agr Org United Nations, Agr & Consumer protect Dept, Vialle Terme Caracalla, I-00153 Rome, Italy. EM pierre.gerber@fao.org RI Kebreab, Ermias/E-9113-2012; Dijkstra, Jan/G-7680-2011 OI Kebreab, Ermias/0000-0002-0833-1352; Dijkstra, Jan/0000-0003-3728-6885 FU MICCA (Mitigation of Climate Change in Agriculture) programme FX The experts that participated in the workshop to peer-review the report by Hristov et al. (2013) and provide insights into interactions between mitigation measures are thanked for their input. They are (in alphabetic order): M. Doreau, R. Eckhardt, D. Hongmin, T. McAllister, H. Montgomery, M. Powell, S. Sommer and M. Tibbo. The authors remain responsible for the content of this article and accountable for any potential factual error. The research underpinning this review was co-financed by the MICCA (Mitigation of Climate Change in Agriculture) programme (http://www.fao.org/climatechange/micca). NR 106 TC 33 Z9 33 U1 14 U2 145 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD JUN PY 2013 VL 7 SU 2 BP 220 EP 234 DI 10.1017/S1751731113000876 PG 15 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA 272ZO UT WOS:000328502300004 PM 23739465 ER PT J AU Del Prado, A Crosson, P Olesen, JE Rotz, CA AF Del Prado, A. Crosson, P. Olesen, J. E. Rotz, C. A. TI Whole-farm models to quantify greenhouse gas emissions and their potential use for linking climate change mitigation and adaptation in temperate grassland ruminant-based farming systems SO ANIMAL LA English DT Article; Proceedings Paper CT 5th Greenhouse Gases and Animal Agriculture (GGAA) Conference CY JUN 23-26, 2013 CL Dublin, IRELAND SP Univ Coll Dublin, Teagasc DE adaptation; farm-models; greenhouse gases; mitigation; ruminants ID LIFE-CYCLE ASSESSMENT; DAIRY PRODUCTION SYSTEMS; NITROUS-OXIDE EMISSIONS; SOIL ORGANIC-MATTER; BEEF-PRODUCTION; ENVIRONMENTAL IMPACTS; METHANE EMISSIONS; CARBON SEQUESTRATION; LIVESTOCK PRODUCTION; GRAZING SYSTEMS AB The farm level is the most appropriate scale for evaluating options for mitigating greenhouse gas (GHG) emissions, because the farm represents the unit at which management decisions in livestock production are made. To date, a number of whole farm modelling approaches have been developed to quantify GHG emissions and explore climate change mitigation strategies for livestock systems. This paper analyses the limitations and strengths of the different existing approaches for modelling GHG mitigation by considering basic model structures, approaches for simulating GHG emissions from various farm components and the sensitivity of GHG outputs and mitigation measures to different approaches. Potential challenges for linking existing models with the simulation of impacts and adaptation measures under climate change are explored along with a brief discussion of the effects on other ecosystem services. C1 [Del Prado, A.] Basque Ctr Climate Change, Bilbao 48008, Spain. [Crosson, P.] TEAGASC, Livestock Syst Res Dept, Anim & Grassland Res & Innovat Ctr, Dunsany, Meath, Ireland. [Olesen, J. E.] Aarhus Univ, Dept Agroecol, DK-8830 Tjele, Denmark. [Rotz, C. A.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Del Prado, A (reprint author), Basque Ctr Climate Change, Alameda Urquijo,4,4-1A, Bilbao 48008, Spain. EM agustin.delprado@bc3research.org RI del Prado, Agustin/B-4675-2010; Azkarate, Ainhoa/K-9087-2013 OI del Prado, Agustin/0000-0003-3895-4478; Azkarate, Ainhoa/0000-0003-0471-3094 FU Spanish National R+D+i Plan [CGL2009-10176]; Basque Department of Education, Universities and Research [PC2010-33A]; EU [266018] FX A. Del Prado would like to thank the Spanish National R+D+i Plan (grant no. CGL2009-10176) and Basque Department of Education, Universities and Research (grant no. PC2010-33A) for funding part of this study. Jorgen E. Olesen acknowledges the EU-FP7 funded AnimalChange project (grant no. 266018) for contributing to the study. NR 97 TC 19 Z9 19 U1 1 U2 43 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD JUN PY 2013 VL 7 SU 2 BP 373 EP 385 DI 10.1017/S1751731113000748 PG 13 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA 272ZO UT WOS:000328502300017 PM 23739478 ER PT J AU Ewbank, AC Ruder, MG McRuer, DL Armien, AG AF Ewbank, Ana Carolina Ruder, Mark G. McRuer, David L. Armien, Anibal G. TI What Is Your Diagnosis? SO JOURNAL OF AVIAN MEDICINE AND SURGERY LA English DT Editorial Material ID RICKETS C1 [Ewbank, Ana Carolina] Univ Minnesota, Coll Vet Med, Raptor Ctr, St Paul, MN 55108 USA. [Ruder, Mark G.] ARS, USDA, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. [McRuer, David L.] Wildlife Ctr Virginia, Waynesboro, VA 22980 USA. [Armien, Anibal G.] Univ Minnesota, Coll Vet Med, Dept Vet Populat Med, Vet Diagnost Lab, St Paul, MN 55108 USA. RP Ewbank, AC (reprint author), Univ Minnesota, Coll Vet Med, Raptor Ctr, 1920 Fitch Ave, St Paul, MN 55108 USA. NR 20 TC 0 Z9 0 U1 0 U2 2 PU ASSOC AVIAN VETERINARIANS PI BOCA RATON PA PO BOX 811720, BOCA RATON, FL 33481 USA SN 1082-6742 EI 1938-2871 J9 J AVIAN MED SURG JI J. Avian Med. Surg. PD JUN PY 2013 VL 27 IS 2 BP 156 EP 161 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA 249GK UT WOS:000326765500010 PM 23971225 ER PT J AU Ganji, S Wubben, MJ Jenkins, JN AF Ganji, Satish Wubben, Martin John Jenkins, Johnie Norton TI Two Simple Methods for the Collection of Individual Life Stages of Reniform Nematode, Rotylenchulus reniformis SO JOURNAL OF NEMATOLOGY LA English DT Article DE host-parasitic relationship; life stages; reniform nematode; Rotylenchulus reniformis; technique ID MELOIDOGYNE-INCOGNITA; HETERODERA-GLYCINES; PARASITISM GENES; COTTON; INTERFERENCE; MANAGEMENT; EXPRESSION; INFECTION AB The sedentary semi-endoparasitic nematode Rotylenchulus reniformis, the reniform nematode, is a serious pest of cotton and soybean in the United States. In recent years, interest in the molecular biology of the interaction between R. reniformis and its plant hosts has increased; however, the unusual life cycle of R. reniformis presents a unique set of challenges to researchers who wish to study the developmental expression of a particular nematode gene or evaluate life stage-specific effects of a specific treatment such as RNA-interference or a potential nematicide. In this report, we describe a simple method to collect R. reniformis juvenile and vermiform adult life stages under in vitro conditions and a second method to collect viable parasitic sedentary females from host plant roots. Rotylenchulus reniformis eggs were hatched over a Baermann funnel and the resultant second-stage juveniles incubated in petri plates containing sterile water at 30 degrees C. Nematode development was monitored through the appearance of fourth-stage juveniles and specific time-points at which each developmental stage predominated were determined. Viable parasitic sedentary females were collected from infected roots using a second method that combined blending, sieving, and sucrose flotation. Rotylenchulus reniformis life stages collected with these methods can be used for nucleic acid or protein extraction or other experimental purposes that rely on life stage-specific data. C1 [Ganji, Satish; Wubben, Martin John; Jenkins, Johnie Norton] Mississippi State Univ, Dept Biochem & Mol Biol, Mississippi State, MS 39762 USA. [Wubben, Martin John; Jenkins, Johnie Norton] USDA ARS, Crop Sci Res Lab, Genet & Precis Agr Res Unit, Mississippi State, MS 39762 USA. RP Ganji, S (reprint author), Mississippi State Univ, Dept Biochem & Mol Biol, Mississippi State, MS 39762 USA. EM Martin.Wubben@ars.usda.gov NR 22 TC 3 Z9 3 U1 3 U2 12 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2013 VL 45 IS 2 BP 87 EP 91 PG 5 WC Zoology SC Zoology GA 250ZZ UT WOS:000326897300001 PM 23833322 ER PT J AU Jagdale, GB Holladay, T Brannen, PM Cline, WO Agudelo, P Nyczepir, AP Noe, JP AF Jagdale, Ganpati B. Holladay, Ted Brannen, P. M. Cline, W. O. Agudelo, P. Nyczepir, A. P. Noe, J. P. TI Incidence and Pathogenicity of Plant-Parasitic Nematodes Associated with Blueberry (Vaccinium spp.) Replant Disease in Georgia and North Carolina SO JOURNAL OF NEMATOLOGY LA English DT Article DE blueberry; host-parasitic relationship; Mesocriconema ornatum; replant disease; ring nematode; Vaccinium spp. ID CRICONEMELLA-XENOPLAX; RING NEMATODE; PEACH; ARKANSAS AB Blueberry replant disease (BRD) is an emerging threat to continued blueberry (Vaccinium spp.) production in Georgia and North Carolina. Since high populations of ring nematode Mesocriconema ornatum were found to be associated with commercially grown blueberries in Georgia, we hypothesized that M. ornatum may be responsible for predisposing blueberry to BRD. We therefore tested the pathogenicity of M. ornatum on 10-wk-old Rabbiteye blueberries (Vaccinium virgatum) by inoculating with initial populations (Pi) of 0 (water control), 10, 100, 1,000. and 10,000 mixed stages of M. ornatum/pot under both greenhouse (25 +/- 2 degrees C) and field microplot conditions. Nematode soil population densities and reproduction rates were assessed 75, 150, 225, and 255, and 75, 150, 225, and 375 d after inoculation (DAI) in both the greenhouse and field experiments, respectively. Plant growth parameters were recorded in the greenhouse and field microplot experiments at 255 and 375 DAI, respectively. The highest M. ornatum population density occurred with the highest Pi level, at 75 and 150 DAI under both greenhouse (P < 0.01) and field (P < 0.01) conditions. However, M. ornatum rate of reproduction increased significantly in pots receiving the lowest Pi level of 10 nematodes/plant compared with the pots receiving Pi levels of 100, 1,000, and 10,000 nematodes 75 DAI. Plant-parasitic nematode populations were determined in commercial blueberry replant sites in Georgia and North Carolina during the 2010 growing season. Mesocriconema ornatum and Dolichodorus spp. were the predominant plant-parasitic nematodes in Georgia and North Carolina, respectively, with M. ornatum occurring in nearly half the blueberry fields sampled in Georgia. Other nematode genera detected in both states included Tylenchorhynchus spp., Hoplolaimus spp., Hemicycliophora spp., and Xiphinema spp. Paratrichodorus spp. was also found only in Georgia. In Georgia, our results indicate that blueberry is a host for M. ornatum and its relationship to BRD warrants further investigation. C1 [Jagdale, Ganpati B.; Holladay, Ted; Brannen, P. M.; Noe, J. P.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Cline, W. O.] N Carolina State Univ, Dept Plant Pathol, Hort Crops Res Stn, Castle Hayne, NC 28429 USA. [Agudelo, P.] Clemson Univ, Sch Agr Forest & Environm Sci, Clemson, SC 29634 USA. [Nyczepir, A. P.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Jagdale, GB (reprint author), Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. EM gbjagdal@uga.edu FU Georgia Blueberry Commodity Commission FX The authors thank Heagan Farms, Manor, GA, for supplying blueberry seedlings; Judy Cross, Charles Laster, and Merry A. Bacon for technical assistance; the Cooperative Extension Agents for their assistance in the survey; and the Georgia Blueberry Commodity Commission for funding. NR 20 TC 0 Z9 0 U1 2 U2 10 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2013 VL 45 IS 2 BP 92 EP 98 PG 7 WC Zoology SC Zoology GA 250ZZ UT WOS:000326897300002 PM 23833323 ER PT J AU Ma, JB Kirkpatrick, TL Rothrock, CS Brye, K AF Ma, Jianbing Kirkpatrick, Terrence L. Rothrock, Craig S. Brye, Kristofor TI Effects of Soil Compaction and Meloidogyne incognita on Cotton Root Architecture and Plant Growth SO JOURNAL OF NEMATOLOGY LA English DT Article DE ecology; host-parasite relationship; root-knot nematode; root topology; soil hardpan ID POTATO-CYST NEMATODES; KNOT NEMATODE; WATER RELATIONS; THIELAVIOPSIS-BASICOLA; PENETRATION RESISTANCE; PARASITIC NEMATODES; ALFALFA SEEDLINGS; GLOBODERA-PALLIDA; SYSTEM MORPHOLOGY; HORDEUM-VULGARE AB The effects of a soil hardpan and Meloidogyne incognita on cotton root architecture and plant growth were evaluated in microplots in 2010 and 2011. Soil was infested with M. incognita at four different levels with or without a hardpan. The presence of a hardpan resulted in increased plant height, number of main stem nodes, and root fresh weight for cotton seedlings both years. Meloidogyne incognita decreased height and number of nodes for seedlings in 2010. Nematode infestation increased seedling root length and enhanced root magnitude, altitude, and exterior path length in 2010. This was also the case for root length and magnitude in 2011 at lower infestation levels suggesting compensatory growth. A hardpan had no consistent effect on these root parameters but increased root volume in both years. A hardpan hastened crop maturity and increased the number of fruiting branches that were produced, while M. incognita infection delayed crop development and reduced plant height and number of bolls. Both M. incognita infection and a hardpan reduced taproot length and root dry weight below the hardpan in both years. Root topological indices under all the treatments ranged from 1.71 to 1.83 both years indicating that root branching followed a herringbone pattern. The techniques for characterizing root architecture that were used in this study provide a greater understanding of changes that result from disease and soil abiotic parameters affecting root function and crop productivity. C1 [Ma, Jianbing] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Kirkpatrick, Terrence L.] Univ Arkansas, Southwest Res & Extens Ctr, Hope, AR 71801 USA. [Rothrock, Craig S.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. [Brye, Kristofor] Univ Arkansas, Dept Soil Crop & Environm Sci, Fayetteville, AR 72701 USA. RP Ma, JB (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, 2890 Highway130 East, Stuttgart, AR 72160 USA. EM tkirkpatrick@uaex.edu FU Arkansas Cotton State Support Committee; Cotton, Inc. [08-326AR] FX This research was supported, in part, by the Arkansas Cotton State Support Committee and Cotton, Inc. (08-326AR). The authors thank Margie Miller, Ronnie Bateman, and Stephanie Crow for technical assistance. NR 58 TC 2 Z9 2 U1 1 U2 9 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD JUN PY 2013 VL 45 IS 2 BP 112 EP 121 PG 10 WC Zoology SC Zoology GA 250ZZ UT WOS:000326897300005 PM 23833326 ER PT J AU Kosler, JS AF Kosler, Joseph S. TI Handbook of Statistical Data Editing and Imputation SO JOURNAL OF OFFICIAL STATISTICS LA English DT Book Review C1 [Kosler, Joseph S.] Natl Agr Stat Serv, USDA, Div Res & Dev, Fairfax, VA 22030 USA. RP Kosler, JS (reprint author), Natl Agr Stat Serv, USDA, Div Res & Dev, 3251 Old Lee Highway, Fairfax, VA 22030 USA. EM Joseph.Kosler@nass.usda.gov RI de Waal, Ton/H-8669-2016 OI de Waal, Ton/0000-0003-1515-3790 NR 7 TC 0 Z9 0 U1 1 U2 1 PU VERSITA PI WARSAW PA SOLIPSKA 14A-1, 02-482 WARSAW, POLAND SN 0282-423X J9 J OFF STAT JI J. Off. Stat. PD JUN PY 2013 VL 29 IS 2 BP 324 EP 327 PG 4 WC Social Sciences, Mathematical Methods; Statistics & Probability SC Mathematical Methods In Social Sciences; Mathematics GA 254AO UT WOS:000327132500009 ER PT J AU Costello, SL Jacobi, WR Negron, JF AF Costello, Sheryl L. Jacobi, William R. Negron, Jose F. TI EMERGENCE OF BUPRESTIDAE, CERAMBYCIDAE, AND SCOLYTINAE (COLEOPTERA) FROM MOUNTAIN PINE BEETLE-KILLED AND FIRE-KILLED PONDEROSA PINES IN THE BLACK HILLS, SOUTH DAKOTA, USA SO COLEOPTERISTS BULLETIN LA English DT Article DE wood borers; bark beetles; metallic wood-boring beetles; longhorn beetles; Dendroctonus ponderosae ID SURROGATE TREATMENTS; 3-TOED WOODPECKERS; PRESCRIBED FIRE; TREE MORTALITY; FOREST; ATTRACTANTS; CALIFORNIA; ABUNDANCE; RESPONSES; INSECTS AB Wood borers (Coleoptera: Cerambycidae and Buprestidae) and bark beetles (Coleoptera: Curculionidae) infest ponderosa pines, Pinus ponderosa P. Lawson and C. Lawson, killed by mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, and fire. No data is available comparing wood borer and bark beetle densities or species guilds associated with MPB-killed or fire-killed trees from the Black Hills of South Dakota, USA. We compared species assemblages of wood borers (Cerambycidae and Buprestidae) and bark beetles (Dendroctonus spp. and Ips spp. (Coleoptera: Curculionidae)) emerging from bolts collected from trees that had been killed by MPB or fire one or two years prior to harvesting. Significantly more wood borers emerged from bolts of fire-killed trees than from bolts of MPB-killed trees. Wood borer density in fire-killed trees was 13.8 per m(2), compared to 4.4 per m(2) in MPB-killed trees. Six wood borer species, Acanthocinus obliquus (LeConte), Buprestis consularis Gory, Chrysobothris sp., Melanophila acuminata (De Geer), Monochamus clamator LeConte, and Phaenops gentilis (LeConte), emerged from fire-killed tree bolts. Four species of wood borers, A. obliquus, Rhagium inquisitor (L.), Tetropium cinnamopterum Kirby, and Xylotrechus longitarsis Casey, emerged from MPB-killed tree bolts. Acanthocinus obliquus was the only species that emerged from both MPB-killed and fire-killed tree bolts. The bark beetles D. ponderosae, Dendroctonus valens LeConte, Ips knausi Swaine, and Ips pini (Say) emerged from one-year-old, MPB-killed trees, while I. pini was the only species that emerged from a fire-killed tree. C1 [Costello, Sheryl L.] USDA Forest Serv, Forest Hlth Management, Denver, CO 80401 USA. [Jacobi, William R.] Colorado State Univ, Dept Bioagricultural Sci & Pest Management, Ft Collins, CO 80523 USA. [Negron, Jose F.] USDA Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80526 USA. RP Costello, SL (reprint author), USDA Forest Serv, Forest Hlth Management, 740 Simms St, Denver, CO 80401 USA. FU USDA Forest Service National Fire Plan Project, Rocky Mountain Research Station, Fort Collins, Colorado [01.RMS.C4] FX We would like to thank John Popp for felling our study trees. We also thank Scott Baggett and Benjamin Bird, Rocky Mountain Research Station, for statistical support and two anonymous reviewers whose comments greatly enhanced this manuscript. Colorado State University provided logistical and scientific support and shade house space. This research was supported by funding from the USDA Forest Service National Fire Plan Project 01.RMS.C4, Rocky Mountain Research Station, Fort Collins, Colorado. NR 30 TC 5 Z9 6 U1 2 U2 19 PU COLEOPTERISTS SOC PI ATHENS PA UNIV GEORGIA, 413 BIOLOGICAL SCIENCES BUILDING, ATHENS, GA 30602-2603 USA SN 0010-065X EI 1938-4394 J9 COLEOPTS BULL JI Coleopt. Bull. PD JUN PY 2013 VL 67 IS 2 BP 149 EP 154 DI 10.1649/0010-065X-67.2.149 PG 6 WC Entomology SC Entomology GA 235HH UT WOS:000325707300014 ER PT J AU Marks, HM Tohamy, SM Tsui, F AF Marks, Harry M. Tohamy, Soumaya M. Tsui, Flora TI Modeling Uncertainty of Estimated Illnesses Attributed to Non-O157:H7 Shiga Toxin-Producing Escherichia coli and Its Impact on Illness Cost SO JOURNAL OF FOOD PROTECTION LA English DT Article ID UNITED-STATES; FOODBORNE ILLNESS; PATHOGENS; BOOTSTRAP; LOSSES AB Because of numerous reported foodborne illness cases due to non-O157:H7 Shiga toxin-producing Escherichia coli (STEC) bacteria in the United States and elsewhere, interest in requiring better control of these pathogens in the food supply has increased. Successfully putting forth regulations depends upon cost-benefit analyses. Policy decisions often depend upon an evaluation of the uncertainty of the estimates used in such an analysis. This article presents an approach for estimating the uncertainties of estimated expected cost per illness and total annual costs of non-O157 STEC-related illnesses due to uncertainties associated with (i) recent FoodNet data and (ii) methodology proposed by Scallan et al. in 2011. The FoodNet data categorize illnesses regarding hospitalization and death. We obtained the illness-category costs from the foodborne illness cost calculator of the U. S. Department of Agriculture, Economic Research Service. Our approach for estimating attendant uncertainties differs from that of Scallan et al. because we used a classical bootstrap procedure for estimating uncertainty of an estimated parameter value (e. g., mean value), reflecting the design of the FoodNet database, whereas the other approach results in an uncertainty distribution that includes an extraneous contribution due to the underlying variability of the distribution of illnesses among different sites. For data covering 2005 through 2010, we estimate that the average cost per illness was about $450, with a 98% credible interval of $230 to $1,000. This estimate and range are based on estimations of about one death and 100 hospitalizations per 34,000 illnesses. Our estimate of the total annual cost is about $51 million, with a 98% credible interval of $19 million to $122 million. The uncertainty distribution for total annual cost is approximated well by a lognormal distribution, with mean and standard deviations for the log-transformed costs of 10.765 and 0.390, respectively. C1 [Marks, Harry M.; Tohamy, Soumaya M.; Tsui, Flora] US Food Safety & Inspect Serv, USDA, Off Policy & Program Dev, Policy Anal Div, Washington, DC 20250 USA. RP Marks, HM (reprint author), US Food Safety & Inspect Serv, USDA, Off Policy & Program Dev, Policy Anal Div, Washington, DC 20250 USA. EM harry.marks@fsis.usda.gov NR 13 TC 5 Z9 5 U1 0 U2 5 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2013 VL 76 IS 6 BP 945 EP 952 DI 10.4315/0362-028X.JFP-12-409 PG 8 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FR UT WOS:000326080900005 PM 23726188 ER PT J AU Oscar, TP Tasmin, R Parveen, S AF Oscar, T. P. Tasmin, R. Parveen, S. TI Chlorine Inactivation of Nonresistant and Antibiotic-Resistant Strains of Salmonella Typhimurium Isolated from Chicken Carcasses SO JOURNAL OF FOOD PROTECTION LA English DT Article ID PREVALENCE; POULTRY AB A study was conducted to test the hypothesis that strains of Salmonella Typhimurium that are resistant to antibiotics are more resistant to chlorine in chilled water than strains of Salmonella Typhimurium that are not resistant to antibiotics. To test this hypothesis, strains (n = 16) of Salmonella Typhimurium with four antibiotic resistance profiles were tested for their inactivation kinetics in chlorinated (30 ppm, pH 6) water at 4 degrees C. The four antibiotic resistance profiles were (i) none; (ii) tetracycline-sulfisoxazole (T-Su); (iii) tetracycline-ampicillin-amoxicillin-cefoxitin-ceftiofur-sulfisoxazole (T-A-Am-C-Ce-Su); and (iv) tetracycline-ampicillin-amoxicillin-cefoxitin-ceftiofur-sulfisoxazole-kanamycin (T-A-Am-C-Ce-Su-K). Inactivation of Salmonella Typhimurium in chlorinated water displayed nonlinear kinetics with a concave downward curve that fit well (R-2 = 0.964) to the power law model, with a shape parameter of 1.37. The time for a single log reduction (D-value) of Salmonella Typhimurium from an initial concentration of 5.36 log/ml did not differ (P > 0.05) among the four antibiotic resistance groups and ranged from 3.8 to 4.3 min for n = 4 strains per group. Thus, the hypothesis was rejected, and it was concluded that expression of an antibiotic resistance phenotype does not confer cross-protection in Salmonella Typhimurium to chlorine inactivation in chilled water. C1 [Oscar, T. P.] ARS, USDA, Residue Chem & Predict Microbiol Res Unit, Ctr Food Sci & Technol, Princess Anne, MD 21853 USA. [Tasmin, R.; Parveen, S.] Univ Maryland Eastern Shore, Food Sci & Technol Program, Dept Agr Food & Resource Sci, Princess Anne, MD 21853 USA. RP Oscar, TP (reprint author), ARS, USDA, Residue Chem & Predict Microbiol Res Unit, Ctr Food Sci & Technol, Room 2111, Princess Anne, MD 21853 USA. EM thomas.oscar@ars.usda.gov FU USDA, Evans-Allen program; USDA, ARS FX The authors thank Tagelsir Mohammad of the University of Maryland Eastern Shore and the USDA, Food Safety and Inspection Service and Jaci Ludwig (retired) of the USDA, Agricultural Research Service (ARS) for helpful suggestions and technical assistance, and the USDA, Evans-Allen program and USDA, ARS for funding this project. NR 15 TC 3 Z9 3 U1 0 U2 7 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2013 VL 76 IS 6 BP 1031 EP 1034 DI 10.4315/0362-028X.JFP-12-499 PG 4 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FR UT WOS:000326080900017 PM 23726200 ER PT J AU Oscar, TP AF Oscar, T. P. TI Validation of a Predictive Model for Survival and Growth of Salmonella Typhimurium DT104 on Chicken Skin for Extrapolation to a Previous History of Frozen Storage SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; IN-GROUND BEEF; POTASSIUM LACTATE; SODIUM DIACETATE; TEMPERATURE; MEAT; INACTIVATION; PRODUCTS; MIXTURE AB The U. S. Department of Agriculture's tertiary Pathogen Modeling Program (PMP) model for survival and growth of Salmonella enterica ser. Typhimurium definitive type 104 (DT104) on chicken skin stored for 0 to 8 h at 5 to 50 degrees C was evaluated for its ability to predict survival and growth of the same organism on chicken skin after frozen storage for 6 days at -220 degrees C. Experimental design and methods used to collect data for model development (dependent data) were the same as those used to collect data for survival and growth after frozen storage (independent data for extrapolation). This was done to provide a valid comparison of observed and predicted values. The model was classified as providing acceptable predictions of the test data when the proportion of residuals in an acceptable prediction zone (pAPZ) from -1 log (fail-safe) to 0.5 log (fail-dangerous) was >= 0.7. The pAPZ for dependent data, independent data for interpolation, and independent data for extrapolation to a new independent variable of previous frozen storage were all acceptable (pAPZ >= 0.7), with the exception of the pAPZ for dependent data at 50 degrees C, where an unacceptable pAPZ of 0.625 was obtained. Although a majority of observed log counts were less than predicted log counts, indicating that frozen storage of chicken skin for 6 days at -20 degrees C had injured some Salmonella Typhimurium DT104, the injury was not large enough to cause the tertiary PMP model to provide unacceptable predictions. Thus, it was concluded that the tertiary PMP model provided valid predictions of survival and growth of Salmonella Typhimurium DT104 on chicken skin that had a previous history of frozen storage for 6 days at -20 degrees C. Additional research is needed to determine how broadly the model can be applied to other conditions of previous frozen storage. C1 Univ Maryland Eastern Shore, Chem Residue & Predict Microbiol Res Unit, Ctr Food Sci & Technol, USDA,ARS, Princess Anne, MD 21853 USA. RP Oscar, TP (reprint author), Univ Maryland Eastern Shore, Chem Residue & Predict Microbiol Res Unit, Ctr Food Sci & Technol, USDA,ARS, Room 2111, Princess Anne, MD 21853 USA. EM thomas.oscar@ars.usda.gov NR 26 TC 2 Z9 2 U1 0 U2 3 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2013 VL 76 IS 6 BP 1035 EP 1040 DI 10.4315/0362-028X.JFP-12-362 PG 6 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FR UT WOS:000326080900018 PM 23726201 ER PT J AU Harris, LJ Berry, ED Blessington, T Erickson, M Jay-Russell, M Jiang, XP Killinger, K Michel, FC Millner, P Schneider, K Sharma, M Suslow, TV Wang, LX Worobo, RW AF Harris, Linda J. Berry, Elaine D. Blessington, Tyann Erickson, Marilyn Jay-Russell, Michele Jiang, Xiuping Killinger, Karen Michel, Fredrick C., Jr. Millner, Pat Schneider, Keith Sharma, Manan Suslow, Trevor V. Wang, Luxin Worobo, Randy W. TI A Framework for Developing Research Protocols for Evaluation of Microbial Hazards and Controls during Production That Pertain to the Application of Untreated Soil Amendments of Animal Origin on Land Used To Grow Produce That May Be Consumed Raw SO JOURNAL OF FOOD PROTECTION LA English DT Article ID ESCHERICHIA-COLI O157-H7; MANURE-AMENDED SOIL; ENTERICA SEROVAR TYPHIMURIUM; SALMONELLA-ENTERICA; AGRICULTURAL SOIL; IRRIGATION WATER; BOVINE MANURE; DAIRY MANURE; COMMUNITY STRUCTURE; INDICATOR BACTERIA AB Application of manure or soil amendments of animal origin (untreated soil amendments; UTSAs) to agricultural land has been a long-standing practice to maintain or improve soil quality through addition of organic matter, nitrogen, and phosphorus. Much smaller quantities of these types of UTSAs are applied to land used for food crops than to land used for animal grain and forage. UTSAs can harbor zoonotic enteric pathogens that may survive for extended periods after application. Additional studies are needed to enhance our understanding of preharvest microbial food safety hazards and control measures pertaining to the application of UTSAs especially for land used to grow produce that may be consumed raw. This document is intended to provide an approach to study design and a framework for defining the scope and type of data required. This document also provides a tool for evaluating the strength of existing data and thus can aid the produce industry and regulatory authorities in identifying additional research needs. Ultimately, this framework provides a means by which researchers can increase consistency among and between studies and facilitates direct comparison of hazards and efficacy of controls applied to different regions, conditions, and practices. C1 [Harris, Linda J.; Blessington, Tyann; Jay-Russell, Michele] Univ Calif Davis, Western Ctr Food Safety, Davis, CA 95616 USA. [Suslow, Trevor V.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Berry, Elaine D.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Erickson, Marilyn] Univ Georgia, Ctr Food Safety, Griffin, GA 30223 USA. [Jiang, Xiuping] Clemson Univ, Dept Food Nutr & Packaging Sci, Clemson, SC 29634 USA. [Killinger, Karen] Washington State Univ, Sch Food Sci, Pullman, WA 99164 USA. [Michel, Fredrick C., Jr.] Ohio State Univ, Dept Food Agr & Biol Engn, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Millner, Pat; Sharma, Manan] ARS, USDA, Beltsville Agr Res Ctr, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Schneider, Keith] Univ Florida, Dept Food Sci & Human Nutr, Gainesville, FL 32611 USA. [Wang, Luxin] Auburn Univ, Dept Anim Sci, Auburn, AL 36849 USA. [Worobo, Randy W.] Cornell Univ, Dept Food Sci, Geneva, NY 14456 USA. RP Harris, LJ (reprint author), Univ Calif Davis, Western Ctr Food Safety, 1 Shields Ave, Davis, CA 95616 USA. EM ljharris@ucdavis.edu RI Worobo, Randy/D-8779-2014; Harris, Linda/B-5030-2011 OI Harris, Linda/0000-0002-1911-752X FU FDA [U01-003-572] FX The development of this document was supported by the FDA (project U01-003-572). We are thankful for the review, comments, and suggestions on the draft document from the following individuals: Francisco Diez-Gonzalez (University of Minnesota, St. Paul), Bob Elliott (Sunkist Growers, Fontana, CA), Hank Giclas (Western Growers Association, Irvine, CA), Dave Gombas (United Fresh, Washington, DC), Maka Hajmeer (California Department of Public Health, Sacramento), Mark Ibekwe (U. S. Department of Agriculture, Agricultural Research Service [USDA-ARS], Riverside, CA), Karl Matthews (Rutgers University, New Brunswick, NJ), Yakov Pachepsky (USDA-ARS, Beltsville, MD), Courtney Parker (True Leaf Farms, Salinas, CA), Max Teplitski (University of Florida, Gainesville), Robert Whitaker (Produce Marketing Association, Newark, DE), and Mike Willett (Northwest Horticultural Council, Yakima, WA). We are also grateful for the editorial assistance of Sylvia Yada. NR 87 TC 11 Z9 11 U1 2 U2 30 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD JUN PY 2013 VL 76 IS 6 BP 1062 EP 1084 DI 10.4315/0362-028X.JFP-13-007 PG 23 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA 240FR UT WOS:000326080900023 PM 23726206 ER PT J AU Donald, PA Allen, PB Tyler, DD Sistani, KR Tewolde, H Walker, ER AF Donald, P. A. Allen, P. B. Tyler, D. D. Sistani, K. R. Tewolde, H. Walker, E. R. TI EFFECT OF BROILER LITTER APPLICATION TO SOYBEAN CROP INFESTED WITH SOYBEAN CYST NEMATODE SO NEMATROPICA LA English DT Article DE broiler litter; management; soybean cyst nematode ID LIQUID SWINE MANURE; N-VIRO SOIL; MELOIDOGYNE-INCOGNITA; HETERODERA-GLYCINES; CHICKEN LITTER; POULTRY LITTER; COVER CROPS; ROOT-KNOT; SUPPRESSION; AMENDMENTS AB Manipulation of the plant root zone to reduce the impact of plant parasitic nematodes has been a goal of researchers. Addition of animal manure has a long history of improved soil quality and reduced soil-borne diseases. The objective of this research was to measure the agronomic benefits of broiler litter application to a soybean (Glycine max) crop under no-till and disk regimes and to assess the impact of broiler litter application on soybean cyst nematode (Heterodera glycines) reproduction. Field treatments included two levels of broiler litter (6.7 Mg ha(-1) and 13.4 Mg ha(-1)). Plots were established in a field where according to University of Tennessee soil tests no P or K were needed. Application of broiler litter at both rates significantly increased plant height in 2008 (P = 0.0001) and also in 2009 (P = 0.002). The benefits of broiler litter for soybean growth as measured by plant height was observed regardless of the litter rate. Significant grain yield differences were observed in 2008 (P = 0.0025). Spectral measurements among treatments were highly significant in 2008 (P = 0.0001). Spectral measurements were highly correlated with plant height, grain yield, and H. glycines egg population density at harvest in 2008. Increased levels of P, Mg and Zn were present in the soil where broiler litter was applied. We did not find a significant effect of broiler litter application on H. glycines reproduction in field trials. C1 [Donald, P. A.] USDA ARS, Crop Genet Res Unit, Jackson, TN USA. [Allen, P. B.] Univ Tennessee, BESS, Knoxville, TN USA. [Tyler, D. D.] Univ Tennessee, BESS, Jackson, TN USA. [Sistani, K. R.] USDA ARS, Anim Waste Management Unit, Bowling Green, KY USA. [Tewolde, H.] USDA ARS, Genet & Precis Agr Res Unit, Mississippi State, MS USA. [Walker, E. R.] Univ Tennessee, Dept Plant Sci, Martin, TN 38238 USA. RP Donald, PA (reprint author), USDA ARS, Crop Genet Res Unit, Jackson, TN USA. EM Pat.Donald@ars.usda.gov NR 40 TC 0 Z9 0 U1 0 U2 15 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 EI 2220-5608 J9 NEMATROPICA JI Nematropica PD JUN PY 2013 VL 43 IS 1 BP 24 EP 34 PG 11 WC Zoology SC Zoology GA 235JE UT WOS:000325713500004 ER PT J AU Kokalis-Burelle, N Hilf, ME Rosskopf, EN AF Kokalis-Burelle, Nancy Hilf, Mark E. Rosskopf, Erin N. TI ASSESSMENT OF PYCNOSTACHYS URTICIFOLIA SUSCEPTIBILITY TO THREE SPECIES OF MELOIDOGYNE SO NEMATROPICA LA English DT Article DE Florida; Meloidogyne spp.; Pycnostachys urticifolia; root-knot nematodes AB An exotic ornamental shrub, Pyncostachys urticifolia, growing in a residential planting in Orange County, Florida, was found to have root galling typical of root-knot nematodes (Meloidogyne spp.). Several gravid female nematodes were isolated from roots and identified as M. arenaria based on their esterase phenotype. Single egg mass cultures were produced and reinoculated onto P. urticofilia grown from seed in sterilized builder's sand in the greenhouse. The identification of subsequent egg masses was confirmed as Meloidogyne arenaria based on the esterase phenotype. Greenhouse experiments were then performed to assess susceptibility of P. urticifolia to two additional root-knot nematode species commonly found in Florida, M. incognita and M. javanica. Nematode galling and reproduction were evaluated for all three Meloidogyne spp. in replicated trials with Solanum lycopersicum (tomato cv. Rutger's) included as a susceptible control. Root galling was similar for P. urticifolia and S. lycopersicum for all three species of Meloidogyne tested, and the number of eggs produced per gram of root tissue was similar for S. lycopersicum and P. urticifolia for two of the three species tested, demonstrating that P. urticifolia is highly susceptible to M. arenaria, M. incognita, and M. javanica. C1 [Kokalis-Burelle, Nancy; Hilf, Mark E.; Rosskopf, Erin N.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Kokalis-Burelle, N (reprint author), ARS, USDA, US Hort Res Lab, 2001 S Rock Rd, Ft Pierce, FL 34945 USA. EM nancy.burelle@ars.usda.gov NR 2 TC 0 Z9 0 U1 0 U2 0 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 EI 2220-5608 J9 NEMATROPICA JI Nematropica PD JUN PY 2013 VL 43 IS 1 BP 40 EP 43 PG 4 WC Zoology SC Zoology GA 235JE UT WOS:000325713500006 ER PT J AU Cabos, RYM Wang, KH Sipes, BS Heller, WP Matsumoto, TK AF Cabos, R. Y. M. Wang, K-H. Sipes, B. S. Heller, W. P. Matsumoto, T. K. TI DETECTION OF PLANT-PARASITIC NEMATODE DNA IN THE GUT OF PREDATORY AND OMNIVOROUS NEMATODES SO NEMATROPICA LA English DT Article DE Aporcelaimellus; burrowing nematode; molecular gut content analysis; Mesodorylaimus; Mononchoides; Mononchus; Neoactinolaimus; plant-parasitic nematodes; predator-prey; predatory nematodes; reniform nematode; root-knot; nematode ID SOIL; PRIMERS AB A protocol for molecular gut analysis of nematodes was developed to determine if predatory and omnivorous nematodes from five different guilds prey on Rotylenchulus reniformis, Meloidogyne incognita, and Radopholus similis. Mononchoides, Mononchus, Neoactinolaimus, Mesodorylaimus, and Aporcelaimellus were collected from Hawaii agroecosystems. Gut contents were released by slicing nematodes and pipetted into a PCR tube. Appropriate speciesspecific primers for the PCR reaction were determined by the predominant plant-parasitic nematodes associated with the source of the predatory and omnivorous nematodes. Predator and omnivore samples containing ingested R. reniformis, M. incognita and R. similis amplified a band of the desired size of 224, 342, and 269 bp, respectively. All predator and omnivore genera tested were positive for plant-parasitic nematode DNA in at least 22% of the specimens assayed. The highest percentage of positive detections (55%) was in Neoactinolaimus. This study confirmed that species-specific PCR primers could detect targeted plant-parasitic nematode prey in the gut of excised nematodes. This protocol could be adopted to further our understanding of the role of nematodes in soil food web interactions. C1 [Cabos, R. Y. M.; Heller, W. P.; Matsumoto, T. K.] ARS, USDA, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Wang, K-H.; Sipes, B. S.] Univ Hawaii Manoa, Dept Plant & Environm Protect Sci, Coll Trop Agr & Human Resources, Honolulu, HI 96822 USA. RP Cabos, RYM (reprint author), ARS, USDA, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM roxana.cabos@ars.usda.gov NR 15 TC 3 Z9 3 U1 3 U2 13 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 EI 2220-5608 J9 NEMATROPICA JI Nematropica PD JUN PY 2013 VL 43 IS 1 BP 44 EP 48 PG 5 WC Zoology SC Zoology GA 235JE UT WOS:000325713500007 ER PT J AU Nyczepir, AP AF Nyczepir, A. P. TI FIELD PERFORMANCE OF PECAN ROOTSTOCKS FOR RESISTANCE TO MELOIDOGYNE PARTITYLA IN THE SOUTHEASTERN UNITED STATES SO NEMATROPICA LA English DT Article DE Carya illinoinensis; host parasitic relationship; management; Meloidogyne partityla; pecan; resistance; root; knot nematode; rootstock; susceptibility. ID PHENOTYPES AB Two open pollinated commercial pecan seedling rootstocks were evaluated for resistance to Meloidogyne partityla in field microplots. Rootstocks tested included seedlings derived from open pollinated seed of ` Curtis' and ` Elliott' parent trees. ` Elliott', a susceptible pecan seedling rootstock for M. partityla, also served as the identifiable control. Both commercial pecan rootstocks supported nematode reproduction as indicated by number of egg masses per plant, number of eggs per plant, and number of eggs per gram dry root and were not significantly different between rootstocks 30 months after inoculation. Differences in tree growth, as measured by trunk diameter and dry root weight, were not found between ` Elliott' and ` Curtis' rootstocks, but growth suppression of both stocks was influenced in the presence of M. partityla. These results provide useful insights into M. partityla being a potentially economically important pathogen to the pecan industry in the southeastern United States and that the search for an IPM strategy for control of M. partityla on pecan is warranted. C1 USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Nyczepir, AP (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM andy.nyczepir@ars.usda.gov NR 25 TC 0 Z9 0 U1 1 U2 2 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 EI 2220-5608 J9 NEMATROPICA JI Nematropica PD JUN PY 2013 VL 43 IS 1 BP 63 EP 67 PG 5 WC Zoology SC Zoology GA 235JE UT WOS:000325713500010 ER PT J AU Looft, T Levine, UY Stanton, TB AF Looft, T. Levine, U. Y. Stanton, T. B. TI Cloacibacillus porcorum sp nov., a mucin-degrading bacterium from the swine intestinal tract and emended description of the genus Cloacibacillus SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID HUMAN ORAL-CAVITY; RIBOSOMAL-RNA; SEQUENCE DATA; HUMAN-ORIGIN; SYNERGISTES; BUTYRATE; GROWTH; ACID; ARB AB A novel anaerobic, mesophilic, amino-acid-fermenting bacterium, designated strain CL-84(T), was isolated from the swine intestinal tract on mucin-based media. Cells were curved rods (0.8-1.2x3.5-5.0 mu m), stained Gram-negative and were non-motile with no evidence of spores. Strain CL-84T produced acetate, propionate, formate and butyrate as the end products of metabolism when grown on serine. Optimum growth occurred at 39 degrees C and pH 6.5. The major cellular fatty acids were iso-C-15:0, iso-C-15:0 3-OH, iso-C-17:0 and C-16:0, distinguishing strain CL-84(T) from closely related species. The DNA G+C content of strain CL-84(T) was 55.1 mol%. 16S rRNA gene sequence analysis showed that strain CL-84(T) shared 90-95% similarity with characterized genera within the phylum Synergistetes, family Synergistaceae. Phylogenetic analysis showed that strain CL-84(T) was related to, but distinct from, Cloacibacillus evryensis. Based on these findings, we propose that strain CL-84(T) represents a novel species of the genus Cloacibacillus. We further propose the name Cloacibacillus porcorum sp. nov. be designated for this species. The type strain is CL-84(T) (=DSM 25858(T)=CCUG 62631(T)). An emended description of the genus Cloacibacillus is provided. C1 [Looft, T.; Levine, U. Y.; Stanton, T. B.] Agr Res Serv, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Stanton, TB (reprint author), Agr Res Serv, Food Safety & Enter Pathogens Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM thad.stanton@ars.usda.gov FU Agricultural Research Service FX The authors thank Sam Humphrey, Deb Lebo, Darrell Bayles and David Alt for scientific advice and technical support. We thank Judi Stasko for the electron microscopy work, Floyd Dewhirst for taxonomic advice, Eric Martens for advice with our glycan purifications, Jonathan Eisen for access to the C. evryensis genome, and Heather Allen, Milt Allison and Nancy Cornick for helpful discussions and comments on the manuscript. Animals were raised in accordance with National Animal Disease Center Animal Care and Use Committee guidelines. This work was supported by the Agricultural Research Service. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 26 TC 9 Z9 10 U1 7 U2 17 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD JUN PY 2013 VL 63 BP 1960 EP 1966 DI 10.1099/ijs.0.044719-0 PN 6 PG 7 WC Microbiology SC Microbiology GA 190NY UT WOS:000322348700005 PM 23041639 ER PT J AU Zeri, M Hussain, MZ Anderson-Teixeira, KJ DeLucia, E Bernacchi, CJ AF Zeri, Marcelo Hussain, Mir Zaman Anderson-Teixeira, Kristina J. DeLucia, Evan Bernacchi, Carl J. TI Water use efficiency of perennial and annual bioenergy crops in central Illinois SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE Water Use Efficiency; biofuels; Carbon balance; Agriculture ID MISCANTHUS X GIGANTEUS; LAND-USE CHANGE; BIOFUEL CROPS; ECOSYSTEM RESPIRATION; CARBON SEQUESTRATION; TEMPERATE CLIMATE; HYDROLOGIC-CYCLE; UNITED-STATES; SOIL CARBON; EXCHANGE AB Sustainable bioenergy production depends upon the efficiency with which crops use available water to produce biomass and store carbon belowground. Therefore, water use efficiency (WUE; productivity vs. annual evapotranspiration, ET) is a key metric of bioenergy crop performance. We evaluate WUE of three potential perennial grass bioenergy crops, Miscanthusxgiganteus (miscanthus), Panicum virgatum (switchgrass), and an assemblage of prairie species (28 species), and Zea mays-Glycine max rotation, during the establishment phase in Illinois. Ecosystem WUE (EWUE; net ecosystem productivity vs. ET) was highest in miscanthus, reaching a maximum value of 12.80.3kgha(-1)mm(-1) in the third year, followed by switchgrass (7.50.3kgha(-1)mm(-1)) and prairie (3.90.3kgha(-1)mm(-1)); the row crop was the lowest. Besides EWUE, harvest-WUE (HWUE, harvested biomass vs. ET) and net biome productivity-WUE (BWUE, calculated as net ecosystem production - harvest vs. ET) were also estimated for all crops and years. After three years of establishment, HWUE and BWUE were highest in miscanthus (9.02 and 3.8 +/- 2.9kgha(-1)mm(-1), respectively) providing a net benefit to the carbon balance, while the row crops had a negative carbon balance and a negative BWUE. BWUE for maize/soybean indicate that this ecosystem would deplete the soil carbon stocks while using the water resources. Switchgrass had the second highest BWUE, while prairie was almost neutral indicating that long-term carbon sequestration for this agro-ecosystem would be sensitive to harvest timing with an early harvest removing more biomass, and thus carbon, from the field. C1 [Zeri, Marcelo; Hussain, Mir Zaman; Anderson-Teixeira, Kristina J.; DeLucia, Evan; Bernacchi, Carl J.] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. [Zeri, Marcelo] Inst Nacl Pesquisas Espaciais, Ctr Ciencia Sistema Terr, BR-12630000 Cachoeira Paulista, SP, Brazil. [Anderson-Teixeira, Kristina J.] Smithsonian Inst Global Earth Observ, Ctr Trop Forest Sci, Front Royal, VA USA. [Anderson-Teixeira, Kristina J.] Smithsonian Conservat Biol Inst, Front Royal, VA USA. [DeLucia, Evan; Bernacchi, Carl J.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [DeLucia, Evan] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL USA. RP Bernacchi, CJ (reprint author), Univ Illinois, Dept Plant Biol, 1201 West Gregory Dr,Room 193, Urbana, IL 61801 USA. EM bernacch@illinois.edu FU Energy Biosciences Institute; FAPESP [2011/04101-0] FX The authors are grateful to the helpful comments and suggestions of three anonymous reviewers. Climate data provided by the Illinois State Water Survey (ISWS) located in Champaign, Illinois, and on the web at www.isws.illinois.edu. This work was funded by the Energy Biosciences Institute. M. Zeri would like to thank FAPESP (process 2011/04101-0) for the support during the preparation of this manuscript. NR 54 TC 17 Z9 18 U1 1 U2 33 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD JUN PY 2013 VL 118 IS 2 BP 581 EP 589 DI 10.1002/jgrg.20052 PG 9 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA 224TU UT WOS:000324913100016 ER PT J AU Anderson, TK Sukhdeo, MVK AF Anderson, Tavis K. Sukhdeo, Michael V. K. TI THE RELATIONSHIP BETWEEN COMMUNITY SPECIES RICHNESS AND THE RICHNESS OF THE PARASITE COMMUNITY IN FUNDULUS HETEROCLITUS SO JOURNAL OF PARASITOLOGY LA English DT Article ID SNAIL INTERMEDIATE HOSTS; FOOD WEBS; LARVAL TREMATODES; SPATIAL HETEROGENEITY; DISEASE RISK; DIVERSITY; BIODIVERSITY; EQUIVALENCE; TESTS; NETWORKS AB This study examined the relationship between the metazoan parasite community in a focal species, Fundulus heteroclitus, and the species richness of the free-living community. Four distinct salt marsh areas were selected in order to reflect a gradient in free-living species richness and time post-restoration (unrestored, 0, 10, and 20 yr post-restoration). A total of 960 fish was studied, with 30 collected per site in each of 8 consecutive seasons between 2005 and 2007. Species richness and abundance of birds, fish, and benthic macroinvertebrates varied significantly between sites. However, increasing richness in the free-living community was neither positively nor negatively correlated with richness in the parasite infracommunity in F. heteroclitus. These results demonstrate that the relationship between community species richness and parasite richness is not linear. The data suggest that the diversity of parasites may be more correlated with measures of community composition, such as the structure and cohesiveness of the food web, rather than with prevailing measures of biodiversity. C1 Rutgers State Univ, Grad Program Ecol & Evolut, New Brunswick, NJ 08901 USA. Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. RP Anderson, TK (reprint author), USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, 1920 Dayton Ave, Ames, IA 50010 USA. EM tavis.anderson@ars.usda.gov OI Anderson, Tavis/0000-0002-3138-5535 FU Willis A. Reid Jr. Student Research Grant (American Society of Parasitologists); Rutgers Foundation Parasite Research Fund FX This research was funded in part by a Willis A. Reid Jr. Student Research Grant (American Society of Parasitologists) to TKA, and by the Rutgers Foundation Parasite Research Fund to TKA and MVKS. NR 65 TC 4 Z9 4 U1 3 U2 23 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2013 VL 99 IS 3 BP 391 EP 396 DI 10.1645/GE-2940.1 PG 6 WC Parasitology SC Parasitology GA 192XR UT WOS:000322521800001 PM 23198795 ER PT J AU Dubey, JP van Wilpe, E Blignaut, DJC Schares, G Williams, JH AF Dubey, J. P. van Wilpe, E. Blignaut, D. J. C. Schares, G. Williams, J. H. TI DEVELOPMENT OF EARLY TISSUE CYSTS AND ASSOCIATED PATHOLOGY OF BESNOITIA BESNOITI IN A NATURALLY INFECTED BULL (BOS TAURUS) FROM SOUTH AFRICA SO JOURNAL OF PARASITOLOGY LA English DT Article ID IN-VITRO ISOLATION; BOVINE BESNOITIOSIS; TOXOPLASMA-GONDII; CATTLE; APICOMPLEXA; PROTOZOA; GERMANY; ULTRASTRUCTURE; MYOFIBROBLAST; REDESCRIPTION AB Besnoitia besnoiti is an apicomplexan that causes serious economic loss in cattle in many countries and the disease is now spreading in Europe. At least 2 phases of bovine besnoitiosis are recognized clinically. An acute febrile phase characterized by anasarca and necrosis of skin is associated with multiplication of tachyzoites in vascular endothelium; this phase is short-lived and rarely diagnosed. Chronic besnoitiosis characterized by dermal lesions is associated with the presence of macroscopic tissue cysts and is easily diagnosed. Here we report the development of early B. besnoiti tissue cysts in a naturally infected Hugenoot bull from South Africa. Tissue cysts were 10-70 mu m in diameter, contained 1-12 bradyzoites, and were most numerous in the dermis, testicles, and pampiniform venous plexus. Amylopectin granules in bradyzoites stained red with periodic acid Schiff (PAS) reaction. Bradyzoites varied in size and in the intensity of PAS reaction (some were PAS-negative), some were plump, and others were slender. With immunohistochemical staining with Besnoitia oryctofelisi and bradyzoite-specific antibodies (BAG-1 made against Toxoplasma gondii bradyzoites), the staining was confined to parasites, and all intracystic organisms were BAG-1 positive. With Gomori's silver stain only bradyzoites were stained very faintly whereas the rest of the tissue cyst was unstained. Ultrastructurally many tissue cysts contained dead bradyzoites and some appeared empty. Unlike bradyzoites from mature cysts, bradyzoites in the present case contained few or no micronemes. These findings are of diagnostic significance. Ultrastructually host cyst cells had features of myofibroblasts, and immunohistochemistry using antibodies against MAC387, lysozyme, vimentin, Von Willebrand factor, and smooth muscle actin confirmed this. Polymerase chain reaction on DNA extracted from lymph node of the bull confirmed B. besnoiti diagnosis. Associated clinical findings, lesions, and histopathology are briefly presented. The bull died of nephrotic syndrome; anasarca in acute besnoitiosis due to protein-losing glomerulopathy is a finding not previously reported in cattle. C1 [Dubey, J. P.; van Wilpe, E.; Blignaut, D. J. C.; Schares, G.; Williams, J. H.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001,BARC East, Beltsville, MD 20705 USA. EM Jitender.dubey@ars.usda.gov OI Schares, Gereon/0000-0002-3217-289X; Williams, June Heather/0000-0002-8542-6451 FU Department of Paraclinical Sciences FX Dr. Sarah Clift, Rephima Phaswane, and Naomi Timmerman are thanked for the non-Besnoitia-specific IMH tissue section staining. Dr. Marco Romito of the Onderstepoort Veterinary Institute is thanked for performing the PCR for the gammaherpes viruses. The Department of Paraclinical Sciences is gratefully acknowledged for financial support. We thank Dr. Shiv Kumar Verma for graphic help. NR 39 TC 16 Z9 16 U1 0 U2 13 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2013 VL 99 IS 3 BP 459 EP 466 DI 10.1645/12-128.1 PG 8 WC Parasitology SC Parasitology GA 192XR UT WOS:000322521800014 PM 23327488 ER PT J AU Alvarado-Esquivel, C Silva-Aguilar, D Villena, I Dubey, JP AF Alvarado-Esquivel, C. Silva-Aguilar, D. Villena, I. Dubey, J. P. TI Seroprevalence of Toxoplasma gondii Infection in Dairy Goats in Michoacan State, Mexico SO JOURNAL OF PARASITOLOGY LA English DT Article ID PREVALENCE; ANTIBODIES; SHEEP; MILK; PIGS AB Seroprevalence of Toxoplasma gondii infection in goats in Michoacan, Mexico, is largely unknown. Antibodies to T. gondii were determined in 341 dairy goats in Michoacan, Mexico, using the modified agglutination test. Goats were raised in 9 farms in 6 municipalities. Overall, antibodies to Toxoplasma were found in 52 (15.2%) of 341 goats, with titers of 1:25 in 16, 1:50 in 9, 1:100 in 4, 1:200 in 4, 1:400 in 4, 1:800 in 9, 1:1,600 in 3, and 1:3,200 or higher in 3. Seropositive goats were found in all 9 farms sampled, and seroprevalence varied significantly among farms (1.9-90%). Seroprevalence of T. gondii varied with age, municipality, altitude, and climate but not with breed. Increased seroprevalence was found in goats aged 13-24 and 49-86 mo old (25% and 22.9%, respectively). Goats raised in farms in a municipality with semi-warm humid climate at 1,700 m of altitude had the highest seroprevalence (62.1%). This is the first report of T. gondii infection in goats in Michoacan State, Mexico, and of an association of seropositivity to T. gondii and semi-warm humid climate. Results indicate that infected goats are likely an important source of infection with T. gondii in humans in Michoacan State. C1 [Alvarado-Esquivel, C.] Juarez Univ Durango State, Fac Med & Nutr, Durango 34000, Mexico. [Silva-Aguilar, D.] Lab Anim Pathol Morelia, Morelia 58240, Michoacan, Mexico. [Villena, I.] Hosp Maison Blanche, Biol Resources Ctr Toxoplasma, Natl Reference Ctr Toxoplasmosis, Lab Parasitol Mycol, F-51092 Reims, France. [Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 16 TC 4 Z9 4 U1 0 U2 8 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 J9 J PARASITOL JI J. Parasitol. PD JUN PY 2013 VL 99 IS 3 BP 540 EP 542 DI 10.1645/12-103.1 PG 3 WC Parasitology SC Parasitology GA 192XR UT WOS:000322521800029 PM 23116086 ER PT J AU Jarret, RL Levy, IJ Potter, TL Cermak, SC AF Jarret, Robert L. Levy, Irvin J. Potter, Thomas L. Cermak, Steven C. TI Seed oil and fatty acid composition in Capsicum spp. SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Food analysis; Food composition; Capsicum spp.; Pepper; Edible fats and oils; Genebank; Germplasm; Genetic diversity; Evaluation; TD-NMR; Biodiversity and nutrition; Cultivar differences; Wild taxa; Cultivated varieties ID ANNUUM L.; PEPPER SEEDS AB The oil content and fatty acid composition of seed of 233 genebank accessions (total) of nine Capsicum species, and a single accession of Tubocapsicum anomalum, were determined. The physicochemical characteristics of oil extracted from seed of Capsicum annuum and Capsicum baccatum were also examined. Significant differences among mean values for seed oil content were detected among the cultivated Capsicum species. Oil content in seed of C. annuum var. annuum was significantly greater than that in seed of other cultivated species. Capsicum pubescens had the lowest average seed oil content. Among the non-cultivated taxa examined, seed of Capsicum galapagoense had the lowest oil content and T. anomalum the highest. Averages across the 5 cultivated taxa for the 4 principal fatty acids were 12.9%, 3.4%, 6.7% and 76.0% for C16:0 (palmitic), C18:0 (stearic), C18:1 (oleic) and 08:2 (linoleic), respectively. Linoleic acid was the principal fatty acid in all samples, with a high value of 81% in Capsicum chinense. Capsicum frutescens had the lowest percentage of total unsaturated fatty acids and T. anomalum the highest. In general, the oil content and fatty acid composition of seed of the wild taxa were similar to those of the cultivated species. (C) 2013 Elsevier Inc. All rights reserved. C1 [Jarret, Robert L.] ARS, USDA, PGRU, Griffin, GA 30224 USA. [Levy, Irvin J.] Gordon Coll, Dept Chem, Wenham, MA 01984 USA. [Potter, Thomas L.] ARS, USDA, SE Watershed Res Lab, Tifton, GA 31793 USA. [Cermak, Steven C.] ARS, USDA, NCAUR, Biooils Res Unit, Peoria, IL 61604 USA. RP Jarret, RL (reprint author), ARS, USDA, PGRU, 1109 Expt St, Griffin, GA 30224 USA. EM bob.jarret@ars.usda.gov; irv.levy@gordon.edu; tom.potter@ars.usda.gov; steven.cermak@ars.usda.gov NR 30 TC 6 Z9 7 U1 5 U2 41 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD JUN PY 2013 VL 30 IS 2 BP 102 EP 108 DI 10.1016/j.jfca.2013.02.005 PG 7 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 221NW UT WOS:000324666600007 ER PT J AU Byrdwell, WC Horst, RL Phillips, KM Holden, JM Patterson, KY Harnly, JM Exler, J AF Byrdwell, William C. Horst, Ronald L. Phillips, Katherine M. Holden, Joanne M. Patterson, Kristine Y. Harnly, James M. Exler, Jacob TI Vitamin D levels in fish and shellfish determined by liquid chromatography with ultraviolet detection and mass spectrometry SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Seafood; Vitamin D; Cholecalciferol; Ergocalciferol; APCI-MS; HPLC; Mass spectrometry; LC-MS; Food analysis; Food composition ID NUTRIENT ANALYSIS PROGRAM; TISSUE DISTRIBUTION; NATIONAL FOOD; HEALTH AB Vitamin D-3 (cholecalciferol) levels were determined in finfish and shellfish using UV detection at 265 nm (combined with auxiliary full scan UV detection) and selected ion monitoring (SIM) mass spectrometry (MS), using vitamin D-2 (ergocalciferol) as an internal standard. Analysis of standard reference material (SRM) NIST 1849 (Infant/Adult Nutritional Formula) was included to validate the method. Three-point calibration curves were employed, allowing values to be determined over a range of species, from those having little or no detectable vitamin D-3 (e.g., pollock, shrimp) to those with high levels (e.g., salmon with up to 33.23 mu g/100 g). The limit of detection (LOD) and limit of quantitation (LOQ) calculated from the uncertainty and intercept of the calibration curves were 1.22 mu g/100 g and 5.30 mu g/100 g, respectively, based on all analyses (n = 27 sequences). Use of response factors (RF) allowed quantitation at lower levels of vitamin D-3, with an LOQ of <0.20 mu g/100 g. The values obtained using the validated methodology agreed well with literature and tabulated database results for most species. However, much lower average vitamin D-3 concentrations were found for oysters (0.05 mu g/100 g, raw) and clams (0.18 mu g/100 g, cooked) compared to other reports for these products. Published by Elsevier Inc. C1 [Byrdwell, William C.; Harnly, James M.] ARS, USDA, BHNRC, FCMDL, Beltsville, MD 20705 USA. [Horst, Ronald L.] Heartland Assays Inc, Ames, IA USA. [Phillips, Katherine M.] Virginia Tech, Food Anal Lab, Dept Biochem, Blacksburg, VA USA. [Holden, Joanne M.; Patterson, Kristine Y.; Exler, Jacob] ARS, USDA, Beltsville Human Nutr Res Ctr, Nutrient Data Lab, Beltsville, MD 20705 USA. RP Byrdwell, WC (reprint author), ARS, USDA, BHNRC, FCMDL, 10300 Baltimore Ave,Bldg 161, Beltsville, MD 20705 USA. EM C.Byrdwell@ARS.USDA.gov OI Byrdwell, William/0000-0001-8241-428X; Phillips, Katherine/0000-0002-4586-8538 NR 29 TC 4 Z9 4 U1 3 U2 38 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD JUN PY 2013 VL 30 IS 2 BP 109 EP 119 DI 10.1016/j.jfca.2013.01.005 PG 11 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA 221NW UT WOS:000324666600008 ER PT J AU Garbarino, VR Campbell, JW O'Brien, J Proctor, HC Dik, B AF Garbarino, Valentina R. Campbell, Joshua W. O'Brien, Joseph Proctor, Heather C. Dik, Bilal TI Phthiraptera and Acari Collected from 13 Species of Waterfowl from Alabama and Georgia SO SOUTHEASTERN NATURALIST LA English DT Article ID LICE INSECTA; TAXONOMIC RICHNESS; FEATHER MITES; BIRDS; MALLOPHAGA; BEHAVIOR; HOST; ECTOPARASITES; MENOPONIDAE; PARASITES AB Waterfowl, including ducks, geese, rails and others, are host to a great diversity of ectosymbiotic arthropods. In this study, we collected ectosymbionts from waterfowl and analyzed taxon richness and total abundance to determine whether there were differences in the mite and louse assemblages of waterfowl of different species, genera, sexes, and feeding behaviors. Data were collected from 53 individual birds from 13 waterfowl species and 5 waterfowl genera taken from Georgia and Alabama. A total of 11 louse species and 7 feather and nasal mite species were collected from the waterfowl samples. Fulica americana (American Coot) harbored the highest louse abundance and taxon richness but had the lowest abundance of mites. Most significant results were driven by the Fulica assemblage. Significant sex differences were detected only between male and female anseriform birds, in which female hosts demonstrated higher mean numbers of ischnoceran lice than male hosts. No significant differences in mite abundance between waterfowl sex or genera were observed, but more mite taxa were found on diving waterfowl than on dabbling species. These data will help to provide a foundation for future research on the ecology of waterfowl ectosymbionts in the southeastern United States. C1 [Garbarino, Valentina R.; Campbell, Joshua W.] High Point Univ, High Point, NC 27262 USA. [O'Brien, Joseph] USDA Forest Serv, Forestry Sci Lab, Athens, GA 30602 USA. [Proctor, Heather C.] Univ Alberta, Edmonton, AB T6G 2E9, Canada. [Dik, Bilal] Selcuk Univ, Vet Fak, Parazitol Anabilim Dali, TR-42250 Selcuklu, Konya, Turkey. RP Garbarino, VR (reprint author), High Point Univ, 833 Montlieu Ave, High Point, NC 27262 USA. EM valentina.garbarino@hotmail.com RI Proctor, Heather/A-2677-2014 NR 50 TC 1 Z9 1 U1 1 U2 11 PU HUMBOLDT FIELD RESEARCH INST PI STEUBEN PA PO BOX 9, STEUBEN, ME 04680-0009 USA SN 1528-7092 J9 SOUTHEAST NAT JI Southeast. Nat. PD JUN PY 2013 VL 12 IS 2 BP 413 EP 426 DI 10.1656/058.012.0214 PG 14 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA 217RR UT WOS:000324378200013 ER PT J AU Olsen, SC AF Olsen, S. C. TI Biosafety considerations for in vivo work with risk group 3 pathogens in large animals and wildlife in North America SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Article DE biosafety; pathogenicity; biocontainment facilities; zoonotic pathogens ID ABORTUS STRAIN RB51; WHITE-TAILED DEER; LABORATORY-ASSOCIATED INFECTIONS; MYCOBACTERIUM-BOVIS; IMMUNE-RESPONSES; ODOCOILEUS-VIRGINIANUS; ERADICATION PROGRAMS; CATTLE; BRUCELLOSIS; TUBERCULOSIS AB Regulations in the United States require animal biosafety level 3 (ABSL-3) or biosafety level 3 agriculture (BSL-3-Ag) containment for many endemic zoonotic pathogens and etiologic agents of foreign animal diseases. In an effort to protect public health, billions of dollars were invested in regulatory programs over many years to reduce the prevalence of zoonotic pathogens such as Brucella and Mycobacterium bovis in domestic livestock. In addition to research needs in domestic livestock hosts, the establishment of brucellosis and tuberculosis in wildlife in the United States has created a need for research studies addressing these zoonotic diseases. As guidelines in the Biosafety in Microbiological and Biomedical Laboratories (BMBL, 2009) for BSL-3 and BSL-3-Ag facilities are primarily directed toward laboratory or vivarium facilities, additional issues should be considered in designing large animal containment facilities for domestic livestock and/or wildlife. Flight distance, herd orientation, social needs, aggressiveness, and predictability are all factors we considered on a species by species basis for designing our containment facilities and for work practices with large ruminants. Although safety risk cannot be completely eliminated when working with large animals, studies in natural hosts are critical for advancing vaccine and diagnostic development, and providing basic knowledge of disease pathogenesis in natural hosts. Data gathered in these types of studies are vital for state and national regulatory personnel in their efforts to design strategies to control or eradicate diseases such as brucellosis and tuberculosis in their natural hosts, whether it is domestic livestock or wildlife. It is likely that failure to address the prevalence of disease in wildlife reservoirs will lead to re-emergence in domestic livestock. The overall benefit of these studies is to protect public health, provide economic benefits to producers, and protect the economic investment made in regulatory programs. C1 USDA ARS, Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA. RP Olsen, SC (reprint author), USDA ARS, Natl Anim Dis Ctr, Infect Bacterial Dis Livestock Res Unit, POB 70, Ames, IA 50010 USA. EM steven.olsen@ars.usda.gov NR 32 TC 0 Z9 0 U1 1 U2 13 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD JUN PY 2013 VL 14 IS 1 BP 2 EP 10 DI 10.1017/S1466252312000217 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA 214IW UT WOS:000324127100002 PM 23286223 ER PT J AU Seal, BS Lillehoj, HS Donovan, DM Gay, CG AF Seal, Bruce S. Lillehoj, Hyun S. Donovan, David M. Gay, Cyril G. TI Alternatives to antibiotics: a symposium on the challenges and solutions for animal production SO ANIMAL HEALTH RESEARCH REVIEWS LA English DT Article DE antimicrobials; immune modulation; gut microbiome; antibiotic growth promoter; pre/probiotics; antibiotic resistance; feed additives; One Health; lytic enzymes; bacteriophage; antimicrobial peptides; phytochemicals ID ANTIMICROBIAL GROWTH PROMOTERS; RESPIRATORY SYNDROME VIRUS; BILE-SALT HYDROLASE; BROILER-CHICKENS; IN-VITRO; BACTERIAL COMMUNITY; GUT MICROBIOTA; CLOSTRIDIUM-PERFRINGENS; BACTERIOPHAGE THERAPY; ANTI-INFECTIVES AB Antibiotics are one of the most important medical discoveries of the 20th century and will remain an essential tool for treating animal and human diseases in the 21st century. However, antibiotic resistance among bacterial pathogens and concerns over their extensive use in food animals has garnered global interest in limiting antibiotic use in animal agriculture. Yet, limiting the availability of medical interventions to prevent and control animal diseases on the farm will directly impact global food security and safety as well as animal and human health. Insufficient attention has been given to the scientific breakthroughs and novel technologies that provide alternatives to antibiotics. The objectives of the symposium 'Alternatives to Antibiotics' were to highlight promising research results and novel technologies that could potentially lead to alternatives to conventional antibiotics, and assess challenges associated with their commercialization, and provide actionable strategies to support development of alternative antimicrobials. The symposium focused on the latest scientific breakthroughs and technologies that could provide new options and alternative strategies for preventing and treating diseases of animals. Some of these new technologies have direct applications as medical interventions for human health, but the focus of the symposium was animal production, animal health and food safety during food-animal production. Five subject areas were explored in detail through scientific presentations and expert panel discussions, including: (1) alternatives to antibiotics, lessons from nature; (2) immune modulation approaches to enhance disease resistance and to treat animal diseases; (3) gut microbiome and immune development, health and diseases; (4) alternatives to antibiotics for animal production; and (5) regulatory pathways to enable the licensure of alternatives to antibiotics. C1 [Seal, Bruce S.] ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. [Lillehoj, Hyun S.; Donovan, David M.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Gay, Cyril G.] ARS, Off Natl Programs, George Washington Carver Ctr, USDA, Beltsville, MD 20705 USA. RP Seal, BS (reprint author), ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, 950 Coll Stn Rd, Athens, GA 30605 USA. EM bruce.seal@ars.usda.gov FU ATA Symposium Organizing Committee FX The authors would like to thank the members of the Alternatives to Antibiotics Symposium Scientific Committee: Sergio Calsamiglia Blancafort, University of Barcelona; Frank Blecha, Kansas State University; Elisabeth Erlacher-Vindel, OIE; Brett Finlay, University of British Columbia; Henk P. Haagsman, Utrecht University; Robert Hancock, University of British Columbia; Filip Van Immerseel, Ghent University; David Mackay, European Medicines Agency and John Wallace, University of Aberdeen. The authors also acknowledge the support provided by the ATA Symposium Organizing Committee and express our sincere thanks to Bernard Vallat and staff at the OIE for hosting the symposium. The helpful guidance of Abigail Charlet with Dodet Biosciences for conference management is greatly appreciated. Note: Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 105 TC 20 Z9 22 U1 7 U2 103 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1466-2523 J9 ANIM HEALTH RES REV JI Anim. Health Res. Rev. PD JUN PY 2013 VL 14 IS 1 BP 78 EP 87 DI 10.1017/S1466252313000030 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA 214IW UT WOS:000324127100007 PM 23702321 ER PT J AU Schetelig, MF Handler, AM AF Schetelig, Marc F. Handler, Alfred M. TI Germline transformation of the spotted wing drosophilid, Drosophila suzukii, with a piggyBac transposon vector SO GENETICA LA English DT Article DE Insect pest management; Sterile insect technique; PiggyBac; Transformation; Female-specific lethality ID CAPITATA DIPTERA TEPHRITIDAE; STERILE INSECT TECHNIQUE; ANASTREPHA-SUSPENSA; CERATITIS-CAPITATA; FRUIT-FLY; EMBRYONIC LETHALITY; SPERM MARKING; SEXING SYSTEM; RECOMBINATION; PROMOTER AB Drosophila suzukii is a pest of small fruits in many parts of the world, whose management is limited to cultural practices and the use of insecticides. Here we describe a method to genetically manipulate this species in the first step to create female lethality strains useful for the sterile insect technique method of population suppression. This was achieved by the germ-line transformation of D. suzukii with a piggyBac transposon vector having a female-specific lethality effector construct. This can be used in a tetracycline-suppressible conditional gene expression system, when crossed to a suitable tet-transactivator strain. Transformation occurred efficiently, at a frequency of 16 % per fertile G0 embryo injected with vector and helper transposase plasmids. The vector was marked for transformant selection with the polyubiquitin-regulated EGFP fluorescent protein, and contains the attP landing site and heterospecific lox recombination sites for post-integration modification of the transgene vector. The 3xP3-AmCyan fluorescent protein marker was inserted within the lox sites to follow a possible recombinase-mediated cassette exchange, that would allow subsequent improvement of the transgenic strain by immobilization of the vector and introduction of new marker cassettes. C1 [Schetelig, Marc F.; Handler, Alfred M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Schetelig, Marc F.] Univ Giessen, D-35392 Giessen, Germany. RP Schetelig, MF (reprint author), Univ Giessen, Heinrich Buff Ring 26-32, D-35392 Giessen, Germany. EM marc.schetelig@agrar.uni-giessen.de; ahandler@ufl.edu FU USDA-National Institute of Food and Agriculture [2011-39211-30769] FX We extend grateful appreciation to Shelley Olson for excellent technical assistance, and to the Biotechnology Risk Assessment Program, USDA-National Institute of Food and Agriculture, for support of this project (Grant No. 2011-39211-30769). NR 25 TC 9 Z9 9 U1 4 U2 57 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 J9 GENETICA JI Genetica PD JUN PY 2013 VL 141 IS 4-6 BP 189 EP 193 DI 10.1007/s10709-013-9717-6 PG 5 WC Genetics & Heredity SC Genetics & Heredity GA 211JG UT WOS:000323902200006 PM 23564446 ER PT J AU Macko-Podgorni, A Nowicka, A Grzebelus, E Simon, PW Grzebelus, D AF Macko-Podgorni, Alicja Nowicka, Anna Grzebelus, Ewa Simon, Philipp W. Grzebelus, Dariusz TI DcSto: carrot Stowaway-like elements are abundant, diverse, and polymorphic SO GENETICA LA English DT Article DE Transposable elements; MITE; Stowaway; Carrot; Polymorphism ID REPEAT TRANSPOSABLE ELEMENTS; DAUCUS-CAROTA L.; MARINER-LIKE TRANSPOSASES; GENOME-WIDE ANALYSIS; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; DNA FRAGMENTS; RICE; MITES; SEQUENCES AB We investigated nine families of Stowaway-like miniature inverted-repeat transposable elements (MITEs) in the carrot genome, named DcSto1 to DcSto9. All of them were AT-rich and shared a highly conserved 6 bp-long TIR typical for Stowaways. The copy number of DcSto1 elements was estimated as ca. 5,000 per diploid genome. We observed preference for clustered insertions of DcSto and other MITEs. Distribution of DcSto1 hybridization signals revealed presence of DcSto1 clusters within euchromatic regions along all chromosomes. An arrangement of eight regions encompassing DcSto insertion sites, studied in detail, was highly variable among plants representing different populations of Daucus carota. All of these insertions were polymorphic which most likely suggests a very recent mobilization of those elements. Insertions of DcSto near carrot genes and presence of putative promoters, regulatory motifs, and polyA signals within their sequences might suggest a possible involvement of DcSto in the regulation of gene expression. C1 [Macko-Podgorni, Alicja; Nowicka, Anna; Grzebelus, Ewa; Grzebelus, Dariusz] Agr Univ Krakow, Dept Genet Plant Breeding & Seed Sci, PL-31425 Krakow, Poland. [Simon, Philipp W.] Univ Wisconsin, USDA ARS Vegetable Crops Res Unit, Dept Hort, Madison, WI 53706 USA. RP Grzebelus, D (reprint author), Agr Univ Krakow, Dept Genet Plant Breeding & Seed Sci, Al 29 Listopada 54, PL-31425 Krakow, Poland. EM dgrzebel@ogr.ar.krakow.pl RI Grzebelus, Dariusz/I-8832-2014 OI Grzebelus, Dariusz/0000-0001-6999-913X FU Polish Ministry of Science and Higher Education [DS3500/KGHN] FX This research was financially supported by the Polish Ministry of Science and Higher Education, project DS3500/KGHN granted to the University of Agriculture in Krakow. NR 53 TC 3 Z9 3 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0016-6707 J9 GENETICA JI Genetica PD JUN PY 2013 VL 141 IS 4-6 BP 255 EP 267 DI 10.1007/s10709-013-9725-6 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA 211JG UT WOS:000323902200013 PM 23775534 ER PT J AU Liu, XM Khajuria, C Li, JR Trick, HN Huang, L Gill, BS Reeck, GR Antony, G White, FF Chen, MS AF Liu, Xuming Khajuria, Chitvan Li, Jiarui Trick, Harold N. Huang, Li Gill, Bikram S. Reeck, Gerald R. Antony, Ginny White, Frank F. Chen, Ming-Shun TI Wheat Mds-1 encodes a heat-shock protein and governs susceptibility towards the Hessian fly gall midge SO NATURE COMMUNICATIONS LA English DT Article ID DISEASE RESISTANCE; PLANT DEFENSE; MOSAIC-VIRUS; BACTERIAL-BLIGHT; TRANSGENIC WHEAT; GENE-EXPRESSION; CYTOSOLIC HSP90; POWDERY MILDEW; COMMON WHEAT; ARABIDOPSIS AB Gall midges induce formation of host nutritive cells and alter plant metabolism to utilize host resources. Here we show that the gene Mayetiola destructor susceptibility-1 on wheat chromosome 3AS encodes a small heat-shock protein and is a major susceptibility gene for infestation of wheat by the gall midge M. destructor, commonly known as the Hessian fly. Transcription of Mayetiola destructor susceptibility-1 and its homoeologs increases upon insect infestation. Ectopic expression of Mayetiola destructor susceptibility-1 or induction by heat shock suppresses resistance of wheat mediated by the resistance gene H13 to Hessian fly. Silencing of Mayetiola destructor susceptibility-1 by RNA interference confers immunity to all Hessian fly biotypes on normally susceptible wheat genotypes. Mayetiola destructor susceptibility-1-silenced plants also show reduced lesion formation due to infection by the powdery mildew fungus Blumeria graminis f. sp. tritici. Modification of susceptibility genes may provide broad and durable sources of resistance to Hessian fly, B. graminis f. sp. tritici, and other pests. C1 [Liu, Xuming; Khajuria, Chitvan; Chen, Ming-Shun] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Li, Jiarui; Trick, Harold N.; Gill, Bikram S.; Antony, Ginny; White, Frank F.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. [Huang, Li] Montana State Univ, Dept Plant Sci & Plant Pathol, Bozeman, MT 59717 USA. [Reeck, Gerald R.] Kansas State Univ, Dept Biochem, Manhattan, KS 66506 USA. [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. RP Chen, MS (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM mchen@ksu.edu OI Trick, Harold/0000-0001-5255-5575 FU USDA [2010-03741] FX We thank Hyeonju Lee, Dehlia McAfee and Juliane Essig for technical assistance in plant transformation, and Drs Robert Bowden, James Campbell and Yoonseong Park for suggestions during manuscript preparation. We also thank Dr Robert Bowden for providing the virulent leaf rust race PRTUS54 and assistance in inoculation and scoring; Dr Sunish K. Sehgal for assistance on searching the best Mds-1 hits from 3BS and 3DS databases. Seeds of Chinese Spring wheat genetic stocks used for Mds-1 gene localization were kindly provided by the Wheat Genetic and Genome Researches Center (WGGRC) at Manhattan, Kansas, USA. The study was partially supported by the USDA grant 2010-03741. Mention of commercial or proprietary product does not constitute endorsement by the USDA. NR 60 TC 14 Z9 14 U1 1 U2 36 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD JUN PY 2013 VL 4 AR 2070 DI 10.1038/ncomms3070 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA 208HS UT WOS:000323669200002 PM 23792912 ER PT J AU Jun, TH Michel, AP Wenger, JA Kang, ST Mian, MAR AF Jun, Tae-Hwan Michel, Andrew P. Wenger, Jacob A. Kang, Sung-Taeg Mian, M. A. Rouf TI Population genetic structure and genetic diversity of soybean aphid collections from the USA, South Korea, and Japan SO GENOME LA English DT Article DE soybean aphid; genetic diversity; differentiation; simple sequence repeats ID RESISTANCE GENES; SSR MARKERS; SHORT READS; MICROSATELLITES; DISCOVERY; GLYCINES; DISTANCE; PROGRAM AB Following its recent invasion of North America, the soybean aphid (Aphis glycines Matsumura) has become the number one insect pest of soybean (Glycine max L. Merr.) in the north central states of the USA. A few studies have been conducted on the population genetic structure and genetic diversity of the soybean aphid and the source of its invasion in North America. Molecular markers, such as simple sequence repeats (SSRs) are very useful in the evaluation of population structure and genetic diversity. We used 18 SSR markers to assess the genetic diversity of soybean aphid collections from the USA, South Korea, and Japan. The aphids were collected from two sites in the USA (Indiana and South Dakota), two sites in South Korea (Yeonggwang district and Cheonan city), and one site in Japan (Utsunomiya). The SSR markers were highly effective in differentiating among aphid collections from different countries. The level of differentiation within each population and among populations from the same country was limited, even in the case of the USA where the two collection sites were more than 1200 km apart. C1 [Jun, Tae-Hwan; Michel, Andrew P.; Wenger, Jacob A.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Entomol, Wooster, OH 44691 USA. [Kang, Sung-Taeg] Dankook Univ, Dept Crop Sci & Biotechnol, Cheonan 330714, South Korea. [Mian, M. A. Rouf] USDA ARS, Wooster, OH 44691 USA. [Mian, M. A. Rouf] Ohio State Univ, Dept Hort & Crop Sci, Wooster, OH 44691 USA. RP Mian, MAR (reprint author), USDA ARS, 1680 Madison Ave, Wooster, OH 44691 USA. EM mian.3@osu.edu FU USDA-ARS; Department of Entomology, The Ohio Agricultural Research and Development Center; Ohio Soybean Council FX We are grateful to F. L. Meng (China), T. Murai (Japan), and J. Jung (South Korea) for providing the soybean aphid samples from native habitats. Funding was provided by USDA-ARS and Department of Entomology, The Ohio Agricultural Research and Development Center, and The Ohio Soybean Council. NR 37 TC 3 Z9 3 U1 2 U2 15 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 1200 MONTREAL ROAD, BUILDING M-55, OTTAWA, ON K1A 0R6, CANADA SN 0831-2796 J9 GENOME JI Genome PD JUN PY 2013 VL 56 IS 6 BP 345 EP 350 DI 10.1139/gen-2013-0027 PG 6 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 203ZH UT WOS:000323333200005 PM 23957674 ER PT J AU Cornax, I Diel, DG Rue, CA Estevez, C Yu, Q Miller, PJ Afonso, CL AF Cornax, Ingrid Diel, Diego G. Rue, Cary A. Estevez, Carlos Yu, Qingzhong Miller, Patti J. Afonso, Claudio L. TI Newcastle disease virus fusion and haemagglutinin-neuraminidase proteins contribute to its macrophage host range SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID PARAMYXOVIRUS FUSION; CLINICOPATHOLOGICAL CHARACTERIZATION; CLEAVAGE SITE; SENDAI VIRUS; VIRULENCE; ACTIVATION; INFECTION; CHICKENS; RECEPTOR; STRAINS AB The fusion (F) and haemagglutinin-neuraminidase (HN) proteins of Newcastle disease virus (NDV) are multifunctional proteins that play critical roles during infection. Here, we assessed the ability of NDV to replicate in macrophages and investigated the contribution of the F and HN proteins to NDV infection/replication in these cells. Results of our study revealed that, while presenting similar replication kinetics in a fibroblast cell line (DF1) or in primary non-adherent splenocytes, the NDV strain CA02 replicates better in macrophages (HD11 and primary adherent splenocytes) than the NDV strain Anhinga/93. Notably, exchange of the HN or both F and HN genes of NDV Anhinga/93 by the corresponding genes from NDV CA02 markedly improved the ability of the chimeric viruses to replicate in macrophages. These results indicate that the F and HN proteins are determinants of NDV macrophage host range. This represents the first description of productive NDV infection in macrophages. C1 [Cornax, Ingrid; Diel, Diego G.; Rue, Cary A.; Estevez, Carlos; Yu, Qingzhong; Miller, Patti J.; Afonso, Claudio L.] USDA ARS, Southeast Poultry Res Lab, Athens, GA 30605 USA. RP Afonso, CL (reprint author), USDA ARS, Southeast Poultry Res Lab, Athens, GA 30605 USA. EM Claudio.Afonso@ars.usda.gov FU USDA [6612-32000-064-00D] FX We thank Dawn Williams-Copplin, Tim Olivier and Xiuqin Xia for technical assistance. This work was supported by USDA funding, CRIS no. 6612-32000-064-00D. NR 22 TC 6 Z9 7 U1 0 U2 3 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD JUN PY 2013 VL 94 BP 1189 EP 1194 DI 10.1099/vir.0.048579-0 PN 6 PG 6 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 170GJ UT WOS:000320838100005 PM 23426356 ER PT J AU Kitikoon, P Nelson, MI Killian, ML Anderson, TK Koster, L Culhane, MR Vincent, AL AF Kitikoon, Pravina Nelson, Martha I. Killian, Mary Lea Anderson, Tavis K. Koster, Leo Culhane, Marie R. Vincent, Amy L. TI Genotype patterns of contemporary reassorted H3N2 virus in US swine SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID INFLUENZA-A VIRUSES; UNITED-STATES; GENETIC EVOLUTION; H1N1 2009; HUMANS; TRANSMISSION; PHYLOGENIES; PIGS AB To understand the evolution of swine-origin H3N2v influenza viruses that have infected 320 humans in the USA since August 2011, we performed a phylogenetic analysis at a whole genome scale of North American swine influenza viruses (n = 200). All viral isolates evolved from the prototypical North American H3 cluster 4 (c4), with evidence for further diversification into subclusters. At least ten distinct reassorted H3N2/pandemic H1 N1 (rH3N2p) genotypes were identified in swine. Genotype 1 (G1) was most frequently detected in swine and all human H3N2v viruses clustered within a single G1 clade. These data suggest that the genetic requirements for transmission to humans may be restricted to a specific subset of swine viruses. Mutations at putative antigenic sites as well as reduced serological cross-reactivity among the H3 subclusters suggest antigenic drift of these contemporary viruses. C1 [Kitikoon, Pravina; Anderson, Tavis K.; Vincent, Amy L.] USDA ARS, Natl Anim Dis Ctr, Virus & Prion Dis Res Unit, Ames, IA 50010 USA. [Nelson, Martha I.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Killian, Mary Lea; Koster, Leo] USDA APHIS, Natl Vet Serv Labs, Ames, IA 50010 USA. [Culhane, Marie R.] Univ Minnesota, Coll Vet Med, Vet Diagnost Lab, St Paul, MN 55108 USA. RP Vincent, AL (reprint author), USDA ARS, Natl Anim Dis Ctr, Virus & Prion Dis Res Unit, POB 70, Ames, IA 50010 USA. EM amy.vincent@ars.usda.gov FU USDA-ARS; USDA-APHIS-VS FX We are grateful to the pork producers and swine veterinarians for participating in the USDA-National Animal Health Laboratory Network (NAHLN) Influenza Virus Surveillance System for swine. We acknowledge Dr Sabrina Swenson (NVSL-USDA-APHIS) and Dr John Korslund (CEAH-USDA-APHIS) for kindly sharing swine influenza genetic and surveillance data from the NAHLN Influenza Virus Surveillance System for swine. Thank you to Alicia Janas-Martindale (NVSL-USDA-APHIS) for assistance in virus isolation whole-genome sequencing. Thank you to Dr Marcus Kehrli (NADC-USDA-ARS) for critical reading of the manuscript. Funding was provided by USDA-ARS and USDA-APHIS-VS. Mention of trade names Or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 24 TC 29 Z9 29 U1 1 U2 14 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD JUN PY 2013 VL 94 BP 1236 EP 1241 DI 10.1099/vir.0.51839-0 PN 6 PG 6 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 170GJ UT WOS:000320838100012 PM 23695819 ER PT J AU Lin, JY Ali, AK Chen, P Ghabrial, S Finer, J Dorrance, A Redinbaugh, P Qu, F AF Lin, Junyan Ali, Ahmed Khamis Chen, Ping Ghabrial, Said Finer, John Dorrance, Anne Redinbaugh, Peg Qu, Feng TI A stem-loop structure in the 5 ' untranslated region of bean pod mottle virus RNA2 is specifically required for RNA2 accumulation SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID REPLICATION; EXPRESSION AB Bean pod mottle virus (BPMV) is a bipartite, positive-sense (+) RNA plant virus of the family Secoviridae. Its RNA1 encodes all proteins needed for genome replication and is capable of autonomous replication. By contrast, BPMV RNA2 must utilize RNA-I-encoded proteins for replication. Here, we sought to identify RNA elements in RNA2 required for its replication. The exchange of 5 ' untranslated regions (UTRs) between genome segments revealed an RNA2-specific element in its 5 ' UTR. Further mapping localized a 66 nucleotide region that was predicted to fold into an RNA stem loop structure, designated SLC. Additional functional analysis indicated the importance of the middle portion of the stem and an adjacent two-base mismatch. This is the first report of a cis-acting RNA element in RNA2 of a bipartite secovirus. C1 [Lin, Junyan; Ali, Ahmed Khamis; Chen, Ping; Dorrance, Anne; Redinbaugh, Peg; Qu, Feng] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Finer, John] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Ali, Ahmed Khamis] Univ Mustansiriyah, Coll Sci, Dept Biol, Baghdad, Iraq. [Chen, Ping] Hainan Univ, Key Lab Protect & Utilizat Trop Crop Germplasm Re, Haikou, Hainan, Peoples R China. [Ghabrial, Said] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Redinbaugh, Peg] ARS, USDA, Corn & Soybean Res Unit, Wooster, OH 44691 USA. RP Qu, F (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, 1680 Madison Ave, Wooster, OH 44691 USA. EM qu.28@osu.edu RI Redinbaugh, Margaret/A-3611-2013; Finer, John/N-4713-2014; OI Finer, John/0000-0001-8004-5468; Ali, Ahmed Khamis/0000-0003-3624-8561 FU North Central Soybean Research Program; Ohio Soybean Council FX We greatly appreciate the generosity of Drs Steve Whitham and John Hill at Iowa State University in providing us with the RNA2-GFP construct. We thank members of the Qu and Finer laboratories for stimulating discussions and technical assistances, Dr Lucy Stewart and other members of the USDA, ARS Corn and Soybean Research Unit for sharing equipment. This study was supported in part by an OARDC Graduate Seed award to J. L. Soybean virus-related research in the Qu lab has been supported in part by grants from the North Central Soybean Research Program and Ohio Soybean Council. We gratefully acknowledge Dr Andy White for critical reading of the manuscript and numerous suggestions. NR 16 TC 3 Z9 3 U1 0 U2 6 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD JUN PY 2013 VL 94 BP 1415 EP 1420 DI 10.1099/vir.0.051755-0 PN 6 PG 6 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 170GJ UT WOS:000320838100032 PM 23388202 ER PT J AU Quito-Avila, DF Brannen, PM Cline, WO Harmon, PF Martin, RR AF Quito-Avila, Diego F. Brannen, Philip M. Cline, William O. Harmon, Philip F. Martin, Robert R. TI Genetic characterization of Blueberry necrotic ring blotch virus, a novel RNA virus with unique genetic features SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID DOUBLE-STRANDED-RNA; MOSAIC-VIRUS; MAXIMUM-LIKELIHOOD; HIGHBUSH BLUEBERRY; SEQUENCE ALIGNMENT; VIRAL REPLICATION; COAT PROTEIN; 3' END; PLANT; MOVEMENT AB A new disorder was observed on southern highbush blueberries in several south-eastern states in the USA. Symptoms included irregularly shaped circular spots or blotches with green centres on the upper and lower surfaces of leaves. Double-stranded RNA was extracted from symptomatic leaves suggesting the presence of virus(es) possibly involved in the disease. Sequencing revealed the presence of a novel RNA virus with similar to 14 kb genome divided into four RNA segments. Sequence analyses showed that the virus, for which we propose the name Blueberry necrotic ring blotch virus (BNRBV), possesses protein domains conserved across RNA viruses in the alphavirus-like supergroup. Phylogenetic inferences using different genes placed BNRBV in a clade that includes the Bromoviridae, the genus Cilevirus (CiLV) and the recently characterized Hibiscus green spot virus (HGSV). Despite the strong genetic relationships found among BNRBV, Cilevirus and HGSV, the genome of BNRBV contains three features that distinguish it significantly from its closest-relatives: (i) the presence of two helicase domains with different evolutionary pathways, (ii) the existence of three conserved nucleotide stretches located at the 3 ' non-coding regions of each RNA segment and (iii) the conservation of terminal nucleotide motifs across each segment. Furthermore, CiLV and HGSV possess poly(A)-tailed bipartite and tripartite genomes, respectively, whereas BNRBV has a quadra-partite genome lacking a poly(A) tail. Based on these genetic features a new genus is proposed for the classification of BNRBV. C1 [Quito-Avila, Diego F.] Escuela Super Politecn Litoral ESPOL, CIBE, EC-090150 Guayaquil, Guayas, Ecuador. [Brannen, Philip M.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Cline, William O.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27607 USA. [Harmon, Philip F.] Univ Florida, Dept Plant Pathol, Gainesville, FL 32605 USA. [Martin, Robert R.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97331 USA. RP Quito-Avila, DF (reprint author), Escuela Super Politecn Litoral ESPOL, CIBE, EC-090150 Guayaquil, Guayas, Ecuador. EM quitod@onid.orst.edu FU Oregon and Washington Blueberry Commissions; British Columbia Blueberry Industry Development Council; Southern Regional Small Fruit Consortium; Georgia Agricultural Commodity Commission for Blueberries; NIFA-Specialty Crops Research Initiative; National Clean Plant Network; USDA-ARS FX The authors acknowledge funding from the Oregon and Washington Blueberry Commissions, the British Columbia Blueberry Industry Development Council, the Southern Regional Small Fruit Consortium, the Georgia Agricultural Commodity Commission for Blueberries, the NIFA-Specialty Crops Research Initiative, the National Clean Plant Network and USDA-ARS. The authors would also like to thank Dr Nicklaus Grunwald and Javier Tabima for providing insight into phylogenetic inferences. NR 52 TC 7 Z9 7 U1 1 U2 10 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 J9 J GEN VIROL JI J. Gen. Virol. PD JUN PY 2013 VL 94 BP 1426 EP 1434 DI 10.1099/vir.0.050393-0 PN 6 PG 9 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA 170GJ UT WOS:000320838100034 PM 23486668 ER PT J AU Ren, Y Wise, ML AF Ren, Y. Wise, M. L. TI Avenanthramide Biosynthesis in Oat Cultivars Treated with Systemic Acquired Resistance Elicitors SO CEREAL RESEARCH COMMUNICATIONS LA English DT Article DE avenanthramides; elicitor; oat; BTH; systemic acquired resistance ID F-SP AVENAE; CROWN RUST; HYDROXYCINNAMOYL-COA; ANTIOXIDANT ACTIVITY; SATIVA; PHYTOALEXINS; POLYPHENOLS; ASSOCIATION; AVENALUMIN; VICTORIN AB The synthetic systemic acquired resistance elicitor benzothiadiazole (BTH) has been shown to elicit avenanthramide biosynthesis in the oat cultivar 'Belle'. This report investigates the response of multiple oat cultivars to BTH as well as 2,6-dichloroisonicotinic acid (INA) at different growth stages. Oat seedlings ('Gem', 'Kame' and 'Ogle') were treated with 2,6-dichloroisonicotinic acid (INA) or benzothiadiazole (BTH) at the three leaf (Z13) stage and subsequently assessed for accumulation of avenanthramides and analyzed for the activities of hydroxycinnamoyl-CoA: hydroxyanthranilate N-hydroycinnamoyl transferase (HHT). In addition, four cultivars ('Belle', 'Gem', 'Kame', and 'Ogle') were treated at the Z43 stage with BTH. The results indicated that after 48 h avenanthramides accumulated to significantly higher levels in the oat leaves in response to both INA and BTH treatment and this response tended to be fairly long lasting (336 h or more). Both elicitors also raised the activities of HHT enzyme in seedling leaves after 48 h. Genotypic differences in the kinetics and quantitative response were also observed in both the seedlings and the mature plants. The magnitude of avenanthramide production in the leaves was somewhat higher in the more mature plants. Filling grain also showed higher levels of avenanthramides compared to the untreated controls. C1 [Ren, Y.; Wise, M. L.] ARS, Cereal Crops Res Unit, USDA, Madison, WI 53726 USA. [Ren, Y.] Shanxi Agr Univ, Coll Agron, Taigu 030801, Peoples R China. RP Wise, ML (reprint author), ARS, Cereal Crops Res Unit, USDA, 502 Walnut St, Madison, WI 53726 USA. EM mlwise@wisc.edu NR 21 TC 1 Z9 1 U1 1 U2 14 PU AKADEMIAI KIADO RT PI BUDAPEST PA PRIELLE K U 19, PO BOX 245,, H-1117 BUDAPEST, HUNGARY SN 0133-3720 J9 CEREAL RES COMMUN JI Cereal Res. Commun. PD JUN PY 2013 VL 41 IS 2 BP 255 EP 265 DI 10.1556/CRC.2012.0035 PG 11 WC Agronomy SC Agriculture GA 172ZF UT WOS:000321045900007 ER PT J AU Pimentel, LA Maia, LA Carvalho, FKD Campos, EM Pfister, JA Cook, D Medeiros, RMT Riet-Correa, F AF Pimentel, Luciano A. Maia, Lisanka A. Carvalho, Fabricio K. de L. Campos, Edipo M. Pfister, James A. Cook, Daniel Medeiros, Rosane M. T. Riet-Correa, Franklin TI Conditioned food aversion for the control of poisoning by Ipomoea carnea subsp fistulosa. SO PESQUISA VETERINARIA BRASILEIRA LA Portuguese DT Article DE Poisonous plants; Ipomoea carnea subsp fistulosa; Convolvulaceae; goats; ruminants; plant poisoning control; aversion technique; lithium chloride ID LYSOSOMAL-STORAGE-DISEASE; GRANDE-DO-SUL; SIDA-CARPINIFOLIA; GOATS; CATTLE; CONVOLVULACEAE; SERICOPHYLLA; SWAINSONINE; RIEDELII AB Conditioned food aversion is a technique that can be used to train livestock to avoid ingestion of poisonous plants. This study tested the efficacy and durability of conditioned food aversion to eliminate goat's consumption of Ipomoea carnea subsp. fistulosa. We used 14 young Moxoto goats, which were initially adapted to the consumption of the plant by offering dried I. carnea subsp. fistulosa with their concentrate diet for 30 days, and then subsequently providing green plant for another 10 days. To confirm the spontaneous consumption of the plant, the goats were allow to graze in a paddock of 510m(2) where I. carnea subsp. fistulosa had been planted in an area of 30m(2) (10 plants/m(2)). On day 42, 12 goats were offered fresh green plant individually in a pen for a few minutes, and after the consumption of any amount of the plant they were treated orally with a solution of LiCl at a dose 175mg per kg of body weight. This procedure was repeated for two more consecutive days. Thereafter, the goats were divided into two groups: Group 1 with four averted and two non-averted goats; and Group 2 with eight averted goats. To verify the efficacy and duration of aversion, both groups were introduced into the paddock with I. carnea subsp. fistulosa three days a week for two hours daily. In Group 1, with two non-averted and four averted goats, all animals started to ingest the plant after 1-6 weeks of grazing. They continually increased their consumption of the plant, but never consumed the plant exclusively. None of the goats of Group 2 goats started eating the plant during the 12 months of observation. After this period the area of the paddock planted with I. carnea subsp. fistulosa was expanded to 80 m(2) and grazing time was increased to four hours per day for five days a week. At this stage all the goats in Group 1 ingested the plant in large quantities. The goats from Group 2 gradually started to eat the plant and aversion was extinguished in all animals after two months. Swainsonine concentration of I. carnea subsp. fistulosa was 0.052 +/- 0.05% (mean +/- SD). It was concluded that conditioned food aversion was effective in reducing goat consumption of I. carnea subsp. fistulosa, but the duration of aversion depends on the time of grazing and amount of plant available. However, the aversion was quickly extinguished by social facilitation when averted animals grazed with non-averted animals. C1 [Pimentel, Luciano A.] Univ Cuiaba UNIC, Hosp Vet, Setor Anat Patol, BR-78065428 Cuiaba, MT, Brazil. [Maia, Lisanka A.; Carvalho, Fabricio K. de L.; Campos, Edipo M.; Medeiros, Rosane M. T.; Riet-Correa, Franklin] Univ Fed Campina Grande, Hosp Vet, Ctr Saude & Tecnol Rural, BR-58708110 Patos de Minas, PB, Brazil. [Pfister, James A.; Cook, Daniel] Utah State Univ, USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Pimentel, LA (reprint author), Univ Cuiaba UNIC, Hosp Vet, Setor Anat Patol, Rua Italia S-N, BR-78065428 Cuiaba, MT, Brazil. EM lucianoanp@yahoo.com.br NR 14 TC 2 Z9 3 U1 1 U2 3 PU REVISTA PESQUISA VETERINARIA BRASILEIRA PI RIO JANEIRO PA EMBRAPA-SAUDE ANIMAL, KM47 SEROPEDICA, 23851-970 RIO JANEIRO, BRAZIL SN 0100-736X J9 PESQUISA VET BRASIL JI Pesqui. Vet. Bras. PD JUN PY 2013 VL 33 IS 6 BP 719 EP 723 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA 191HQ UT WOS:000322404400005 ER PT J AU Abad, JA Li, R Fuentes, S Kreuze, JF Loschinkohl, C Bandla, P AF Abad, J. A. Li, R. Fuentes, S. Kreuze, J. F. Loschinkohl, C. Bandla, P. TI Interception and identification by deep sequencing of a "caulimo-like" virus in a potato germplasm accession imported from South America SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Abad, J. A.] USDA APHIS PPQ PGQP, Beltsville, MD USA. [Li, R.] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD USA. [Fuentes, S.; Kreuze, J. F.] Int Potato Ctr, Lima, Peru. [Loschinkohl, C.; Bandla, P.] USDA APHIS PPQ FO, Plant Germplasm Quarantine Program, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 1 EP 1 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500002 ER PT J AU Abawi, GS Porch, TC Kelly, JD AF Abawi, G. S. Porch, T. C. Kelly, J. D. TI Field evaluation of promising breeding lines and varieties of common bean for tolerance to soilborne pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Abawi, G. S.] Cornell Univ, Geneva, NY USA. [Porch, T. C.] USDA ARS TARS, Mayaguez, PR USA. [Kelly, J. D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 1 EP 1 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500003 ER PT J AU Acimovic, SG VanWoerkom, AH Garavaglia, T Vandervoort, C Wise, JC Sundin, GW AF Acimovic, S. G. VanWoerkom, A. H. Garavaglia, T. Vandervoort, C. Wise, J. C. Sundin, G. W. TI Control of apple scab (Venturia inaequalis) using trunk injection of biopesticides and fungicides in apple trees SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Acimovic, S. G.; VanWoerkom, A. H.; Vandervoort, C.; Wise, J. C.; Sundin, G. W.] Michigan State Univ, E Lansing, MI 48824 USA. [Garavaglia, T.] USDA, E Lansing, MI USA. NR 0 TC 0 Z9 0 U1 2 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 2 EP 2 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500009 ER PT J AU Adams, G Winton, L AF Adams, G. Winton, L. TI Building an index of pathogens of alder SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Adams, G.] Univ Nebraska, Lincoln, NE USA. [Winton, L.] USDA Forest Serv, Anchorage, AK USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 2 EP 3 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500010 ER PT J AU Ajayi, OO Paulitz, TC Campbell, KG Murphy, KM Ullrich, SE AF Ajayi, O. O. Paulitz, T. C. Campbell, K. G. Murphy, K. M. Ullrich, S. E. TI Identification of resistance to Rhizoctonia root rot in mutant and wild barley (Hordeum vulgare subsp spontaneum) SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Ajayi, O. O.; Murphy, K. M.; Ullrich, S. E.] Washington State Univ, Pullman, WA 99164 USA. [Paulitz, T. C.; Campbell, K. G.] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 3 EP 3 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500015 ER PT J AU Al Rwahnih, M Dave, A Anderson, MM Rowhani, A Uyemoto, JK Sudarshana, MR AF Al Rwahnih, M. Dave, A. Anderson, M. M. Rowhani, A. Uyemoto, J. K. Sudarshana, M. R. TI Association of a DNA virus with grapevines affected by red blotch disease in northern California SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Al Rwahnih, M.; Anderson, M. M.; Rowhani, A.] Univ Calif Davis, Davis, CA 95616 USA. [Dave, A.; Uyemoto, J. K.; Sudarshana, M. R.] USDA ARS, Davis, CA USA. NR 0 TC 1 Z9 1 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 4 EP 4 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500017 ER PT J AU Almader, A Callicott, K Probst, C Bandyopadhyay, R Cotty, P AF Almader, A. Callicott, K. Probst, C. Bandyopadhyay, R. Cotty, P. TI Mechanisms of atoxigenicity: Characterizing genetic properties of atoxigenic A. flavus isolates SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Almader, A.; Callicott, K.; Cotty, P.] USDA ARS, Tucson, AZ USA. [Probst, C.] Washington State Univ, Richland, WA USA. [Bandyopadhyay, R.] Int Inst Trop Agr, Ibadan, Nigeria. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 5 EP 5 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500024 ER PT J AU Almeyda, CV Abad, JA Pesic-VanEsbroeck, Z AF Almeyda, C. V. Abad, J. A. Pesic-VanEsbroeck, Z. TI Detection and identification of sweetpotato viruses in North Carolina SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Almeyda, C. V.; Pesic-VanEsbroeck, Z.] N Carolina State Univ, Raleigh, NC 27695 USA. [Abad, J. A.] USDA, APHIS PPQ FO PGQP, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 2 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 5 EP 5 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500025 ER PT J AU Anco, D Poole, G Gottwald, T AF Anco, D. Poole, G. Gottwald, T. TI The effect of postharvest treatments on survival of Xanthomonas citri pv. citri on infected grapefruit leaves SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Anco, D.] N Carolina State Univ, USDA ARS, Ft Pierce, FL USA. [Poole, G.; Gottwald, T.] USDA ARS, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 6 EP 6 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500032 ER PT J AU Bacon, CW Hinton, DM AF Bacon, C. W. Hinton, D. M. TI Additional hosts for Balansia epichloe in tall fescue pastures SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Bacon, C. W.] ARS, USDA, Athens, GA USA. [Hinton, D. M.] ARS, USDA, Russell Res Ctr, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 11 EP 11 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500056 ER PT J AU Baldwin, TT Glenn, AE AF Baldwin, T. T. Glenn, A. E. TI Nitric oxide detoxification by Fusarium verticillioides involves flavohemoglobins and the denitrification pathway SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Baldwin, T. T.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Glenn, A. E.] ARS, USDA, Richard B Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 11 EP 11 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500057 ER PT J AU Baucom, D Cook, D Creamer, R AF Baucom, D. Cook, D. Creamer, R. TI New swainsonine producing Undifilum species from the locoweed Astragalus pubentissimus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Baucom, D.; Creamer, R.] New Mexico State Univ, Las Cruces, NM 88003 USA. [Cook, D.] ARS, USDA, Logan, UT USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 12 EP 12 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500066 ER PT J AU Baumgartner, K Travadon, R Galarneau, E McElrone, A AF Baumgartner, K. Travadon, R. Galarneau, E. McElrone, A. TI Defining the stages of infection of grapevine stems with the trunk disease Botryosphaeria dieback SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Baumgartner, K.; Galarneau, E.; McElrone, A.] ARS, USDA, Davis, CA USA. [Travadon, R.] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 13 EP 13 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500069 ER PT J AU Beirn, LA Tredway, L Boehm, M Orshinsky, A Putman, A Carbone, I Clarke, B Crouch, JA AF Beirn, L. A. Tredway, L. Boehm, M. Orshinsky, A. Putman, A. Carbone, I. Clarke, B. Crouch, J. A. TI A new name for an age-old fungus: Unraveling the mystery of dollar spot disease of turfgrass SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Beirn, L. A.; Clarke, B.] Rutgers State Univ, New Brunswick, NJ 08903 USA. [Tredway, L.] Syngenta Crop Protect, Greensboro, NC USA. [Boehm, M.] Ohio State Univ, Columbus, OH 43210 USA. [Orshinsky, A.] Agr & Agri Food Canada, Saskatoon, SK, Canada. [Putman, A.; Carbone, I.] N Carolina State Univ, Raleigh, NC 27695 USA. [Crouch, J. A.] ARS, USDA, Systemat Mycol & Microbiol Lab, Beltsville, MD USA. NR 0 TC 3 Z9 3 U1 1 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 14 EP 14 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500075 ER PT J AU Bekoscke, KA Asalf, B Stensvand, A Tronsmo, AM Seem, R Peres, N Cadle-Davidson, L Brewer, MT Gadoury, D AF Bekoscke, K. A. Asalf, B. Stensvand, A. Tronsmo, A. M. Seem, R. Peres, N. Cadle-Davidson, L. Brewer, M. T. Gadoury, D. TI Geographic and climatic discontinuity in production of cleistothecia in Podosphaera aphanis SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Bekoscke, K. A.; Seem, R.; Gadoury, D.] Cornell Univ, Geneva, NY USA. [Asalf, B.; Stensvand, A.; Tronsmo, A. M.] Bioforsk, As, Norway. [Peres, N.] Univ Florida, Wimauma, FL USA. [Cadle-Davidson, L.] USDA ARS, GGRU, Geneva, NY USA. [Brewer, M. T.] Univ Georgia, Athens, GA 30602 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 14 EP 14 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500077 ER PT J AU Berner, DK Smallwood, EL Cavin, CA Lagopodi, AL Kashefi, JN Kolomiets, T Pankratova, L Mukhina, Z Cripps, M Bourdot, G AF Berner, D. K. Smallwood, E. L. Cavin, C. A. Lagopodi, A. L. Kashefi, J. N. Kolomiets, T. Pankratova, L. Mukhina, Z. Cripps, M. Bourdot, G. TI Successful biological control of Canada thistle (Cirsium arvense) with the rust fungus Puccinia punctiformis SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Berner, D. K.] USDA ARS, Foreign Dis Weed Sci Res Unit, Frederick, MD USA. [Smallwood, E. L.; Cavin, C. A.] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD USA. [Lagopodi, A. L.] Aristotle Univ Thessaloniki, Sch Agr, GR-54006 Thessaloniki, Greece. [Kashefi, J. N.] European Biol Control Lab, Thessaloniki, Greece. [Kolomiets, T.; Pankratova, L.] All Russia Phytopathol Res Inst, Moscow, Russia. [Mukhina, Z.] All Russia Rice Res Inst, Krasnodar, Russia. [Cripps, M.; Bourdot, G.] AgResearch, Lincoln Res Ctr, Christchurch, New Zealand. RI Bourdot, Graeme/J-7582-2013 NR 0 TC 0 Z9 0 U1 0 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 15 EP 15 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500079 ER PT J AU Blacutt, A Gold, S Mitchell, TR AF Blacutt, A. Gold, S. Mitchell, T. R. TI Genomic and biochemical characterization of cyclic lipopeptides of Bacillus mojavensis RRC101, an antagonist of Fusarium verticillioides SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Blacutt, A.] Univ Georgia, Athens, GA 30602 USA. [Gold, S.; Mitchell, T. R.] USDA ARS, Athens, GA 30613 USA. NR 0 TC 0 Z9 0 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 16 EP 16 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500087 ER PT J AU Block, C Shepherd, L AF Block, C. Shepherd, L. TI Relationship between resistance to Stewart's wilt and Goss's wilt in dent corn inbreds SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Block, C.] USDA ARS, Ames, IA USA. [Shepherd, L.] Iowa State Univ, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 17 EP 17 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500090 ER PT J AU Bock, CH Graham, JH Gottwald, TR AF Bock, C. H. Graham, J. H. Gottwald, T. R. TI A comparison of culture and bioassay for detecting citrus canker SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Bock, C. H.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Graham, J. H.] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL USA. [Gottwald, T. R.] USDA ARS, USHRL, Ft Pierce, FL USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 17 EP 17 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500092 ER PT J AU Bock, CH Hotchkiss, MW Cottrell, TE Wood, BW AF Bock, C. H. Hotchkiss, M. W. Cottrell, T. E. Wood, B. W. TI Fungicide spray coverage from ground-based sprayers in mature pecan trees SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Bock, C. H.; Hotchkiss, M. W.; Cottrell, T. E.; Wood, B. W.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 17 EP 17 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500093 ER PT J AU Bock, CH Brenneman, TB Hotchkiss, MW Wood, BW AF Bock, C. H. Brenneman, T. B. Hotchkiss, M. W. Wood, B. W. TI Trunk applications of phosphite for the control of pecan scab SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Bock, C. H.; Hotchkiss, M. W.; Wood, B. W.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Brenneman, T. B.] Univ Georgia, Coastal Plain Expt Stn, Dept Plant Pathol, Tifton, GA 31793 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 17 EP 17 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500091 ER PT J AU Bonde, MR Murphy, CA Bauchan, GR Luster, DG Palmer, CL Nester, SE Berner, DK AF Bonde, M. R. Murphy, C. A. Bauchan, G. R. Luster, D. G. Palmer, C. L. Nester, S. E. Berner, D. K. TI Systemic infection in chrysanthemum plants by Puccinia horiana, causal agent of chrysanthemum white rust SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Bonde, M. R.; Luster, D. G.; Nester, S. E.; Berner, D. K.] USDA ARS, Frederick, MD USA. [Murphy, C. A.; Bauchan, G. R.] USDA ARS, Beltsville, MD USA. [Palmer, C. L.] Rutgers State Univ, Princeton, NJ USA. NR 0 TC 1 Z9 1 U1 1 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 18 EP 18 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500098 ER PT J AU Bowman, HM Liu, Z Levy, L Nakhla, M AF Bowman, H. M. Liu, Z. Levy, L. Nakhla, M. TI CANARY and LiNK technologies for rapid detection of plant pathogens SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Bowman, H. M.; Liu, Z.; Nakhla, M.] USDA APHIS PPQ S&T CPHST, Beltsville, MD USA. [Levy, L.] USDA APHIS PPQ CPHST, Riverdale, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 20 EP 20 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500105 ER PT J AU Bull, CT Trent, MA Hayes, RJ AF Bull, C. T. Trent, M. A. Hayes, R. J. TI Internal colonization of lettuce leaves by Xanthomonas campestris pv. vitians is influenced by lettuce cultivar SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Bull, C. T.; Trent, M. A.; Hayes, R. J.] ARS, USDA, Salinas, CA USA. NR 0 TC 2 Z9 2 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 21 EP 21 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500114 ER PT J AU Cadle-Davidson, L Nowogrodzki, A Schaub, M Reisch, B Luby, J Hemstad, P Seem, R Gadoury, D AF Cadle-Davidson, L. Nowogrodzki, A. Schaub, M. Reisch, B. Luby, J. Hemstad, P. Seem, R. Gadoury, D. TI Quantitative phenotyping of powdery mildew resistance in grapevine reveals differences in host resistance biology SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Cadle-Davidson, L.] ARS, USDA, GGRU, Geneva, NY USA. [Nowogrodzki, A.; Schaub, M.; Seem, R.; Gadoury, D.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY USA. [Reisch, B.] Cornell Univ, Geneva, NY USA. [Luby, J.; Hemstad, P.] Univ Minnesota, St Paul, MN 55108 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 22 EP 22 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500118 ER PT J AU Caiazzo, R Xiao, CL AF Caiazzo, R. Xiao, C. L. TI Stability and fitness of pyrimethanil-resistant phenotypes of Penicillium expansum from apple SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Caiazzo, R.] Washington State Univ, Wenatchee, WA USA. [Xiao, C. L.] ARS, USDA, Parlier, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 22 EP 23 PG 2 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500119 ER PT J AU Castroagudin, VL Cartwright, RD Jia, M Jackson, AK Fjellstrom, RG Correa-Victoria, FJ Correll, JC AF Castroagudin, V. L. Cartwright, R. D. Jia, M. Jackson, A. K. Fjellstrom, R. G. Correa-Victoria, F. J. Correll, J. C. TI QTL mapping to identify new sources of resistance to rice sheath blight in recombinant inbred lines from the cross of two elite Indica cultivars SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Castroagudin, V. L.] Univ Arkansas, Fayetteville, AR 72701 USA. [Cartwright, R. D.] Univ Arkansas, Cooperat Extens Ctr, Little Rock, AR 72204 USA. [Jia, M.] USDA ARS, Dale Bumper Natl Rice Res Ctr, Stuttgart, AR USA. [Jackson, A. K.; Fjellstrom, R. G.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR USA. [Correa-Victoria, F. J.] Rice Tec Inc, Alvin, TX USA. [Correll, J. C.] Univ Arkansas, Dept Plant Pathol, Fayetteville, AR 72701 USA. NR 0 TC 0 Z9 0 U1 1 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 24 EP 24 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500127 ER PT J AU Cheng, M Dong, J Lee, IM Bottner-Parker, KD Zhao, Y Davis, RE Laski, PJ Mcbeath, JH AF Cheng, M. Dong, J. Lee, I. M. Bottner-Parker, K. D. Zhao, Y. Davis, R. E. Laski, P. J. Mcbeath, J. H. TI Diverse phytoplasma strains, including 16SrXII-E and two new subgroups, associated with diseased potatoes (Solanum tuberosum) in China SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Cheng, M.] Univ Alaska Fairbanks, Agr & Forestry Expt Stati, Plant Pathol & Biotechnol Lab, Fairbanks, AK USA. [Dong, J.] Yunnan Acad Agr Sci, Yunnan Key Lab Agr Biotechnol, Biotechnol & Genet Germplasm Inst, Kunming, Peoples R China. [Lee, I. M.; Bottner-Parker, K. D.; Zhao, Y.; Davis, R. E.] USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Laski, P. J.] Univ Alaska Fairbanks, Plant Pathol & Biotechnol Lab, Agr & Forestry Expt Stn, Fairbanks, AK USA. [Mcbeath, J. H.] Univ Alaska Fairbanks, Fairbanks, AK USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 26 EP 26 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500138 ER PT J AU Cifuentes, J Cappello, S Guzman, G Lodge, J AF Cifuentes, J. Cappello, S. Guzman, G. Lodge, J. TI Cantharocybe brunneovelutina Lodge, Ovrebo et Aime in Mexico SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Cifuentes, J.] Univ Nacl Autonoma Mexico, Fac Ciencias, Coyoacan, DF, Mexico. [Cappello, S.] Univ Juarez Autonoma Tabasco, Div Acad Ciencias Biol, Villahermosa, Tabasco, Mexico. [Guzman, G.] Inst Nacl Ecol Xalapa, Xalapa, Veracruz, Mexico. [Lodge, J.] USDA Forest Serv, Ctr Forest Mycol Res, No Res Stn, Luquillo, PR USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 28 EP 28 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500150 ER PT J AU Clifford, J Kidarsa, T Buchanan, A Chang, JH Loper, J AF Clifford, J. Kidarsa, T. Buchanan, A. Chang, J. H. Loper, J. TI Using next-generation sequencing to determine the influence of metabolic intermediates on the Pseudomonas protegens transcriptome SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Clifford, J.; Kidarsa, T.; Loper, J.] ARS, USDA, HCRL, Corvallis, OR USA. [Buchanan, A.; Chang, J. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 28 EP 28 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500152 ER PT J AU Comstock, JC Sood, S Raid, RN AF Comstock, J. C. Sood, S. Raid, R. N. TI History of brown rust of sugarcane in Florida SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Comstock, J. C.; Sood, S.] ARS, USDA, Sugarcane Field Stn, Canal Point, FL USA. [Raid, R. N.] Univ Florida, Everglades Res & Educ Ctr, IFAS, Belle Glade, FL 33430 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 29 EP 29 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500155 ER PT J AU Copes, WE AF Copes, W. E. TI Spread potential of binucleate Rhizoctonia from propagation floors to trays containing stem cuttings SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Copes, W. E.] ARS, USDA, Poplarville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 30 EP 30 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500160 ER PT J AU Costanzo, S Yang, P Zeller, KA Nakhla, MK AF Costanzo, S. Yang, P. Zeller, K. A. Nakhla, M. K. TI Novel PCR-RFLP assay for genetic diversity studies of Elsinoe australis isolates causing scab on citrus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Costanzo, S.; Yang, P.; Zeller, K. A.; Nakhla, M. K.] USDA APHIS PPQ CPHST, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 30 EP 30 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500164 ER PT J AU Costanzo, S Zeller, KA Nakhla, MK AF Costanzo, S. Zeller, K. A. Nakhla, M. K. TI A conventional PCR and qPCR assays to detect Harpophora maydis-The causal agent of late wilt of corn SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Costanzo, S.; Zeller, K. A.; Nakhla, M. K.] APHIS, USDA, PPQ, CPHST, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 30 EP 30 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500163 ER PT J AU Crutcher, FK Liu, J Bell, AA Stipanovic, RD Kenerley, CM AF Crutcher, F. K. Liu, J. Bell, A. A. Stipanovic, R. D. Kenerley, C. M. TI Analysis of transporter responsible for the secretion of fusaric acid from the plant pathogen Fusarium oxysporum f. sp vasinfectum SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Crutcher, F. K.; Liu, J.; Bell, A. A.; Stipanovic, R. D.] ARS, USDA, College Stn, TX USA. [Kenerley, C. M.] Texas A&M Univ, College Stn, TX USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 31 EP 31 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500169 ER PT J AU Dale, AL Everhart, SE Feau, N Bilodeau, GJ Grunwald, NJ Hamelin, RC AF Dale, A. L. Everhart, S. E. Feau, N. Bilodeau, G. J. Grunwald, N. J. Hamelin, R. C. TI Genome-wide patterns of diversity in four lineages of the sudden oak death pathogen, Phytophthora ramorum SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Dale, A. L.; Feau, N.; Hamelin, R. C.] Univ British Columbia, Vancouver, BC V5Z 1M9, Canada. [Everhart, S. E.] Oregon State Univ, Corvallis, OR 97331 USA. [Bilodeau, G. J.] Canadian Food Inspect Agcy, Ottawa, ON, Canada. [Grunwald, N. J.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 NR 0 TC 0 Z9 0 U1 0 U2 16 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 32 EP 32 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500173 ER PT J AU Daley, JD Hassell, RL Wechter, WP AF Daley, J. D. Hassell, R. L. Wechter, W. P. TI Mapping Alternaria cucumerina resistance in Cucumis melo SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Daley, J. D.; Hassell, R. L.] Clemson Univ, Charleston, SC USA. [Wechter, W. P.] ARS, US Vegetable Lab, USDA, Charleston, SC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 32 EP 32 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500174 ER PT J AU Daniels, J Schneider, WL Fletcher, J Ochoa-Corona, FM AF Daniels, J. Schneider, W. L. Fletcher, J. Ochoa-Corona, F. M. TI Next generation sequencing and its application as a biosecurity tool SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Daniels, J.; Fletcher, J.; Ochoa-Corona, F. M.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W. L.] USDA ARS, FDWSRU, Ft Detrick, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 33 EP 33 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500180 ER PT J AU Daniels, J Stobbe, A Espindola, A Schneider, W Sallee, J Blagden, T Corona, FO Garzon, C Fletcher, J AF Daniels, J. Stobbe, A. Espindola, A. Schneider, W. Sallee, J. Blagden, T. Corona, F. Ochoa Garzon, C. Fletcher, J. TI CSI in a tomato disease plot: Engaging 4-H youth and educators in STEM through investigative plant pathology SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT Joint Meeting of the American-Phytopathological-Society (APS) and the Mycological-Society-of-America (MSA) CY AUG 10-14, 2013 CL Austin, TX SP Amer Phytopathol Soc (APS), Mycol Soc Amer (MSA) C1 [Daniels, J.; Stobbe, A.; Espindola, A.; Sallee, J.; Blagden, T.; Corona, F. Ochoa; Garzon, C.; Fletcher, J.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Schneider, W.] USDA ARS, Ft Detrick, MD USA. RI Espindola, Andres/A-5056-2016 OI Espindola, Andres/0000-0002-9658-0673 NR 0 TC 0 Z9 0 U1 0 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 33 EP 33 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500179 ER PT J AU Danies, G Martin, F Myers, K Cooke, DE Smart, CD Seaman, A Fry, WE AF Danies, G. Martin, F. Myers, K. Cooke, D. E. Smart, C. D. Seaman, A. Fry, W. E. TI Investigation of a population of Pythophthora infestans in and near central New York in 2011 SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Danies, G.; Myers, K.; Smart, C. D.; Fry, W. E.] Cornell Univ, Ithaca, NY USA. [Martin, F.] USDA ARS, Salinas, CA USA. [Cooke, D. E.] James Hutton Inst, Dundee, Scotland. [Seaman, A.] Cornell Univ, Geneva, NY USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 33 EP 33 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500181 ER PT J AU Lara, AD Mosier, N Martin, RR AF Lara, A. Diaz Mosier, N. Martin, R. R. TI Evidence for exogenous and endogenous forms of Rubus yellow net virus in Rubus SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Lara, A. Diaz] Oregon State Univ, Corvallis, OR 97331 USA. [Mosier, N.] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA. [Martin, R. R.] USDA ARS, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 2 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 35 EP 35 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500192 ER PT J AU Dugan, FM Lupien, SL Vahling-Armstrong, CM Chastagner, GA Schroeder, BK AF Dugan, F. M. Lupien, S. L. Vahling-Armstrong, C. M. Chastagner, G. A. Schroeder, B. K. TI Host range of Penicillium spp. (blue mold) rotting bulb crops SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Dugan, F. M.; Lupien, S. L.] ARS, USDA, WRPIS, Pullman, WA USA. [Vahling-Armstrong, C. M.; Schroeder, B. K.] Washington State Univ, Pullman, WA 99164 USA. [Chastagner, G. A.] Washington State Univ, Puyallup, WA USA. NR 0 TC 1 Z9 1 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 37 EP 37 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500201 ER PT J AU Dung, JKS Walenta, DL Alderman, SC Hamm, PB AF Dung, J. K. S. Walenta, D. L. Alderman, S. C. Hamm, P. B. TI Spatial patterns of ergot in fields of Kentucky bluegrass and perennial ryegrass grown for seed in the Columbia Basin of Oregon and Washington SO PHYTOPATHOLOGY LA English DT Meeting Abstract CT APS-MSA Joint Meeting CY AUG 10-14, 2013 CL Austin, TX SP APS, MSA C1 [Dung, J. K. S.; Hamm, P. B.] Oregon State Univ, Hermiston, OR USA. [Walenta, D. L.] Oregon State Univ, La Grande, OR USA. [Alderman, S. C.] ARS, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X J9 PHYTOPATHOLOGY JI Phytopathology PD JUN PY 2013 VL 103 IS 6 SU 2 BP 37 EP 37 PG 1 WC Plant Sciences SC Plant Sciences GA 196TM UT WOS:000322799500203 ER EF