FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Hua, SST Beck, JJ Sarreal, SBL Gee, W AF Hua, Sui Sheng T. Beck, John J. Sarreal, Siov Bouy L. Gee, Wai TI The major volatile compound 2-phenylethanol from the biocontrol yeast, Pichia anomala, inhibits growth and expression of aflatoxin biosynthetic genes of Aspergillus flavus SO MYCOTOXIN RESEARCH LA English DT Article DE Aspergillus flavus; Aflatoxin; Biocontrol; Yeast; Volatile compound; Epigenic control ID MYCOTOXINS; PARASITICUS; CLUSTER AB Aspergillus flavus is a ubiquitous saprophyte that is able to produce the most potent natural carcinogenic compound known as aflatoxin B-1 (AFB(1)). This toxin frequently contaminates crops including corn, cotton, peanuts, and tree nuts causing substantial economic loss worldwide. Consequently, more than 100 countries have strict regulations limiting AFB(1) in foodstuffs and feedstuffs. Plants and microbes are able to produce volatile compounds that act as a defense mechanism against other organisms. Pichia anomala strain WRL-076 is a biocontrol yeast currently being tested to reduce AF contamination of tree nuts in California. We used the SPME-GC/MS analysis and identified the major volatile compound produced by this strain to be 2-phenylethanol (2-PE). It inhibited spore germination and AF production of A. flavus. Inhibition of AF formation by 2-PE was correlated with significant down regulation of clusteringAF biosynthesis genes as evidenced by several to greater than 10,000-fold decrease in gene expression. In a time-course analysis we found that 2-PE also altered the expression patterns of chromatin modifying genes, MYST1, MYST2, MYST3, gcn5, hdaA and rpdA. The biocontrol capacity of P. anomala can be attributed to the production of 2-PE, which affects spore germination, growth, toxin production, and gene expression in A. flavus. C1 [Hua, Sui Sheng T.; Beck, John J.; Sarreal, Siov Bouy L.; Gee, Wai] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Hua, SST (reprint author), ARS, USDA, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM sylvia.hua@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Services [5325-42000-038-00, 5325-42000-037-00] FX This work is supported by in-house research program funds from U.S. Department of Agriculture, Agricultural Research Services (Project Numbers: 5325-42000-038-00 and 5325-42000-037-00). We thank Glenn Bartley and Thomas McKeon for comments. NR 23 TC 14 Z9 15 U1 3 U2 27 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0178-7888 EI 1867-1632 J9 MYCOTOXIN RES JI Mycotoxin Res. PD MAY PY 2014 VL 30 IS 2 BP 71 EP 78 DI 10.1007/s12550-014-0189-z PG 8 WC Mycology; Toxicology SC Mycology; Toxicology GA CS4WY UT WOS:000362078100002 PM 24504634 ER PT J AU Maragos, CM AF Maragos, C. M. TI Production of anti-idiotype antibodies for deoxynivalenol and their evaluation with three immunoassay platforms SO MYCOTOXIN RESEARCH LA English DT Article DE Deoxynivalenol; Antibody; Immunoassay; Biosensor; Fluorescence polarization ID FLUORESCENCE POLARIZATION IMMUNOASSAY; ANTI-IDIOTYPE ANTIBODIES; MYCOTOXIN DEOXYNIVALENOL; MONOCLONAL-ANTIBODY; FUMONISIN B-1; NIVALENOL; AFLATOXIN; VACCINES; UPDATE; TOXINS AB Immunoassays for deoxynivalenol (DON) that involve binding to DON-specific antibodies have been widely developed. In such assays, the responses of samples are generally compared with calibration curves generated by using DON in competition with labeled reagents such as enzymatic or fluorescent conjugates of the toxin. However, materials that mimic the toxin can also be used, provided that they compete effectively with the labeled reagents for the DON-specific antibodies. Examples include certain types of anti-idiotype antibodies, obtained by the immunization of animals with toxin-specific antibodies. In the present work, anti-idiotype antibodies were developed which mimicked DON in the ability to bind to a DON-specific monoclonal antibody (Mab). Fab fragments of the Mab (Ab1) were used to immunize rabbits. Sera were screened by competitive direct enzyme linked immunosorbent assay (CD-ELISA) for the presence of anti-idiotype antibodies (Ab2). In order to determine the most effective screening format and also the potential efficacy in various forms of biosensors, the sera were further evaluated in biolayer interferometry (BLI) and fluorescence polarization immunoassay (FPIA) formats. All three formats were used to demonstrate the presence of anti-idiotypes capable of binding to the paratope of the DON antibody (subtypes Ab2 beta or Ab2 gamma). Such materials have the potential to replace DON as calibrants in immunoassays for this toxin. C1 ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Maragos, CM (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM chris.maragos@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service FX This project was supported by research funds from the U.S. Department of Agriculture, Agricultural Research Service. We thank Candace Lindgren, Jenny Wiersma, Julie Havlicek, Deanna Morrison, Paula Dodge, Nahla Elmahdy, and Shannon Snodgrass of Harlan Bioproducts for Science, for the exceptional quality of the technical assistance provided throughout this project. NR 27 TC 6 Z9 6 U1 5 U2 23 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0178-7888 EI 1867-1632 J9 MYCOTOXIN RES JI Mycotoxin Res. PD MAY PY 2014 VL 30 IS 2 BP 103 EP 111 DI 10.1007/s12550-014-0190-6 PG 9 WC Mycology; Toxicology SC Mycology; Toxicology GA CS4WY UT WOS:000362078100005 PM 24526340 ER PT J AU Wang, B Zhang, GH Shi, YY Zhang, XC AF Wang, Bing Zhang, Guang-Hui Shi, Yang-Yang Zhang, X. C. TI Soil detachment by overland flow under different vegetation restoration models in the Loess Plateau of China SO CATENA LA English DT Article DE Soil erosion; Soil detachment capacity; Overland flow; Vegetation restoration; The Loess Plateau ID DIFFERENT LAND USES; CONCENTRATED FLOW; EROSION MODEL; PLANT-ROOTS; SHALLOW FLOW; HYDRAULIC-PROPERTIES; SEDIMENT TRANSPORT; SOUTHERN ARIZONA; DRAINAGE BASINS; GULLY EROSION AB Land use change has significant effects on soil properties and vegetation cover and thus probably affects soil detachment by overland flow. Few studies were conducted to evaluate the effect of restoration models on the soil detachment process in the Loess Plateau where a Grain for Green Project has been implemented in the past fourteen years. This study was performed to study the effects of vegetation restoration models on soil detachment by overland flow and soil resistance to rill erosion as reflected by rill erodibility and critical shear stress. The undisturbed soil samples were collected from five 37-year-restored lands of abandoned farmland, korshinsk peashrub land (Caragana korshinskill Kom.), black locust land (Robinia pseudoacacia Linn.), Chinese pine land (Pinus tabuliformis Carr.) and mixed forest land of amorpha and Chinese pine. The samples were subjected to flow scouring in a 4.0 m long by 0.35 m wide hydraulic flume under six different shear stresses ranging from 5.60 to 18.15 Pa. The results showed that the measured soil detachment capacities were affected significantly by the restoration models. The mean detachment capacity of cultivated farmland was 23.2 to 55.3 times greater than those of the restored or converted lands. Abandoned farmland showed maximum soil detachment capacity and was 1.02 to 2.29 times greater than the other four restored lands. Soil detachment capacity of the restored lands was significantly influenced by shear stress, cohesion, bulk density, total porosity and root mass density. Detachment capacities were negatively related to cohesion (p < 0.01) with linear function and root mass density (p < 0.05) with exponential function, but positively to total porosity (p < 0.01) with linear function. The rill erodibility would be negatively related to cohesion (p < 0.01) with power function. Besides, the low rill erodibility in the restored lands always had a low soil detachment capacity, while the critical shear stress in the restored lands varied non-monotonically with detachment capacity. The mixed forest land of amorpha and Chinese pine was considered as the best restoration model for its important role in reducing soil detachment capacity. (C) 2013 Elsevier B.V. All rights reserved. C1 [Wang, Bing; Zhang, Guang-Hui] Northwest A&F Univ, Inst Water & Soil Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. [Wang, Bing; Zhang, Guang-Hui] Beijing Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Shi, Yang-Yang] Shanxi Architectural Coll, Taiyuan 030006, Shanxi, Peoples R China. [Zhang, X. C.] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. RP Zhang, GH (reprint author), Northwest A&F Univ, Inst Water & Soil Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. EM bwang@ms.iswc.ac.cn; ghzhang@bnu.edu.cn FU Hundred Talents Project of the Chinese Academy of Sciences; National Natural Science Foundation of China [41271287, 41301295]; Open Fund from State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau [K318009902-1313] FX Financial assistance for this work was provided by the Hundred Talents Project of the Chinese Academy of Sciences, National Natural Science Foundation of China (No. 41271287 and No. 41301295), and the Open Fund from State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau (K318009902-1313). The authors thank the members of the Ansai Research Station of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources for the technical assistance. NR 71 TC 16 Z9 18 U1 3 U2 79 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD MAY PY 2014 VL 116 BP 51 EP 59 DI 10.1016/j.catena.2013.12.010 PG 9 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA AC2TF UT WOS:000332356200005 ER PT J AU Hunter, WJ Manter, DK AF Hunter, William J. Manter, Dan K. TI Antimicrobial Properties of an Oxidizer Produced by Burkholderia cenocepacia P525 SO CURRENT MICROBIOLOGY LA English DT Article ID CEPACIA AB A compound with both oxidizing properties and antibiotic properties was extracted and purified from broth cultures of Burkholderia cenocepacia strain P525. A four step purification procedure was used to increase its specific activity similar to 400-fold and to yield a HPLC-UV chromatogram containing a single major peak. Size exclusion chromatography suggests a molecular mass of similar to 1,150 and UV spectroscopy suggests the presence of a polyene structure consisting of as many as six conjugated double bonds. Biological studies indicate that the compound is bacteriostatic. Enterobacter soli and E. aerogenes cells incubated with the compound exhibit a longer lag phase of growth. The bacteriostatic activity is greater at pH 3 than at pH 5. Bacteria such as B. cenocepacia strain P525 may have value in the agricultural industry as biocontrol agents. C1 [Hunter, William J.; Manter, Dan K.] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. RP Hunter, WJ (reprint author), USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA. EM william.hunter@ars.usda.gov NR 13 TC 0 Z9 0 U1 0 U2 7 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0343-8651 EI 1432-0991 J9 CURR MICROBIOL JI Curr. Microbiol. PD MAY PY 2014 VL 68 IS 5 BP 610 EP 614 DI 10.1007/s00284-013-0515-2 PG 5 WC Microbiology SC Microbiology GA AC3XR UT WOS:000332455400007 PM 24384626 ER PT J AU Evenhuis, JP Wiens, GD Wheeler, P Welch, TJ LaPatra, SE Thorgaard, GH AF Evenhuis, Jason P. Wiens, Gregory D. Wheeler, Paul Welch, Timothy J. LaPatra, Scott E. Thorgaard, Gary H. TI Transfer of serum and cells from Yersinia ruckeri vaccinated doubled-haploid hot creek rainbow trout into outcross Fl progeny elucidates mechanisms of vaccine-induced protection SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Yersinia ruckeri; Rainbow trout; Adoptive transfer; Passive immunization; IgM ID ONCORHYNCHUS-MYKISS WALBAUM; GINBUNA CRUCIAN CARP; GENE-EXPRESSION; BIOTYPE 2; IMMUNITY; IMMUNIZATION; CHALLENGE; DISEASE; IDENTIFICATION; RESPONSES AB Yersinia ruckeri is a well-established bacterial pathogen for many salmonid species, against which a formalin-killed bacterin vaccine has been effective in reducing disease outbreaks. Previous studies have reported conflicting results about the protective value of the systemic humoral response to Y. ruckeri vaccination. Here we directly demonstrate that plasma contains the long-term protective component elicited by both immersion and intraperitoneal injection vaccination of rainbow trout. A total of 0.5 IlL of plasma from vaccinated fish provided almost complete protection against experimental challenge. Conversely, the cells obtained from peripheral blood conferred little or no protection in na ve recipients. The protective component of immune sera was IgM based on size exclusion chromatography and recognition by monoclonal antibody Warr 1-14. Immune plasma generated against a Y. ruckeri biotype 1 strain protected equally against challenges with Y. ruckeri biotype 1 and 2 strains. These results illustrate the importance of the humoral IgM response against Y. ruckeri and the use of doubled haploid rainbow trout (Oncorhynchus mykiss) and transfer of plasma/serum and cells into Fl outcross progeny as a model system for dissection of the mechanism(s) of vaccine-induced protection. Published by Elsevier Ltd. C1 [Evenhuis, Jason P.; Wiens, Gregory D.; Welch, Timothy J.] ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. [Wheeler, Paul; Thorgaard, Gary H.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Wheeler, Paul; Thorgaard, Gary H.] Washington State Univ, Ctr Reprod Biol, Pullman, WA 99164 USA. [LaPatra, Scott E.] Clear Springs Foods Inc, Div Res, Buhl, ID 83316 USA. RP Evenhuis, JP (reprint author), ARS, USDA, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM jason.evenhuis@ars.usda.gov FU Agricultural Research Service CRIS Project [1930-32000-005] FX The authors thank Ryan Lipscomb, Jen Harper and Travis Moreland for expert technical assistance and Mark L. Richardson for critical comments on the manuscript. This work was supported by Agricultural Research Service CRIS Project 1930-32000-005 "Host-Pathogen and Environmental Interactions in Cool and Cold Water Aquaculture". Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. NR 39 TC 3 Z9 3 U1 2 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD MAY PY 2014 VL 44 IS 1 BP 145 EP 151 DI 10.1016/j.dci.2013.12.004 PG 7 WC Immunology; Zoology SC Immunology; Zoology GA AC4OP UT WOS:000332500900018 PM 24342572 ER PT J AU Li, C Beck, BH Peatman, E AF Li, Chao Beck, Benjamin H. Peatman, Eric TI Nutritional impacts on gene expression in the surface mucosa of blue catfish (Ictalurus furcatus) SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Fish; Mucosal; Feed deprivation; Flavobacterium columnare; RNA-seq; Immune response ID AEROMONAS-HYDROPHILA INFECTION; SHORT-TERM STARVATION; SALMON SALMO-SALAR; RNA-SEQ DATA; CHANNEL CATFISH; EDWARDSIELLA-ICTALURI; FLAVOBACTERIUM-COLUMNARE; ATLANTIC SALMON; NITRIC-OXIDE; DIFFERENTIAL EXPRESSION AB Short-term feed deprivation is a common occurrence in both wild and farmed fish species, due to reproductive processes, seasonal variations in temperature, or in response to a disease outbreak. Fasting can have dramatic physiological and biological consequences for fish, including impacts on mucosal immunity which can, in turn, change host susceptibility to pathogens. Culture and selection of blue catfish (ktalurus furcatus) has gained importance as the production of a channel catfish x blue catfish (ktalurus punctatus x I. furcatus) hybrid has increased in the Southeast US. Following a recent examination of fasting-induced impacts on mucosal immunity in channel catfish, here we utilized Illumina-based RNA-seq expression profiling to compare changes in blue catfish gill and skin after a brief (7 day) period of fasting. Transcriptome sequencing and de novo assembly of over 194 million 100 base-pair transcript reads was followed by differential expression analysis. Fasting altered a total of 530 genes in the surface mucosa, including genes regulating the immune response, energy metabolism, mucus production, cellular cytoskeletal structure, cell proliferation, and antioxidant responses. In particular, fasting perturbed arginine synthesis and metabolism pathways in a manner likely altering macrophage activation states and immune readiness. Our findings highlight key mediators of the critical interaction between nutrition and immunity at points of pathogen adherence and entry. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Li, Chao; Peatman, Eric] Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. [Beck, Benjamin H.] ARS, USDA, Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Peatman, E (reprint author), Auburn Univ, Sch Fisheries Aquaculture & Aquat Sci, Auburn, AL 36849 USA. EM peatmer@auburn.edu NR 69 TC 11 Z9 11 U1 2 U2 34 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD MAY PY 2014 VL 44 IS 1 BP 226 EP 234 DI 10.1016/j.dci.2013.12.014 PG 9 WC Immunology; Zoology SC Immunology; Zoology GA AC4OP UT WOS:000332500900027 PM 24378224 ER PT J AU Taylor, JM Seilheimer, TS Fisher, WL AF Taylor, Jason M. Seilheimer, Titus S. Fisher, William L. TI Downstream fish assemblage response to river impoundment varies with degree of hydrologic alteration SO HYDROBIOLOGIA LA English DT Article DE Flow alteration; Flow regimes; Dams; Impoundments; Fish assemblages; Fluvial specialists ID COMMUNITY STRUCTURE; UNITED-STATES; GREAT-PLAINS; FLOW REGIME; ENVIRONMENTAL FLOWS; WATER MANAGEMENT; PRAIRIE STREAMS; CLIMATE-CHANGE; PATTERNS; BIODIVERSITY AB River impoundments can fundamentally restructure downstream fish assemblages by altering flow regimes. However, the degree of alteration and associated ecological change may depend on pre-existing hydrologic regimes. We used long-term datasets to compare downstream hydrological and fish assemblage responses to impoundment in two catchments classified as having intermittent and perennial-flashy natural hydrologic regimes. We observed significant shifts in fish assemblage structure at both sites after stream impoundment. The historically intermittent stream shifted to a stable perennial flow regime. Changes in fish assemblage structure covaried with changes in five different components of the flow regime; most species that increased in abundance require fluvial habitats and likely benefited from increased flows during historically low flow seasons. Shifts in fish assemblage structure were also observed in the perennial stream, despite minimal flow alteration after impoundment; however, most species shifts were associated with lentic environments, and were more likely related to proximity of reservoirs in the drainage system rather than changes in stream flow. Findings from this study confirm that downstream fish assemblage response to river impoundment can be associated with high levels of hydrologic alteration, but other factors including expansion of lentic species into lotic environments also influence shifts in assemblage structure. C1 [Taylor, Jason M.; Seilheimer, Titus S.; Fisher, William L.] Cornell Univ, Dept Nat Resources, New York Cooperat Fish & Wildlife Res Unit, Ithaca, NY 14853 USA. RP Taylor, JM (reprint author), ARS, USDA, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, 598 McElroy Dr, Oxford, MS 38655 USA. EM jason.taylor@ars.usda.gov FU U.S. Geological Survey-U.S. Fish and Wildlife Service, Science Support Partnership Program; Oklahoma and New York Cooperative Fish and Wildlife Research Units FX We thank Ellen Tejan of Enogex, LCC for providing us with complied fish records for the State of Oklahoma. Ryan A. McManamay, David Hoeinghaus, and two anonymous reviewers provided helpful comments that improved this paper. Support for this study was provided by the U.S. Geological Survey-U.S. Fish and Wildlife Service, Science Support Partnership Program and the Oklahoma and New York Cooperative Fish and Wildlife Research Units. The New York Cooperative Fish and Wildlife Research Unit is a cooperative effort of the U.S. Geological Survey, Cornell University, the New York State Department of Environmental Conservation, the U.S. Fish and Wildlife Service, and the Wildlife Management Institute. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 69 TC 4 Z9 4 U1 5 U2 52 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0018-8158 EI 1573-5117 J9 HYDROBIOLOGIA JI Hydrobiologia PD MAY PY 2014 VL 728 IS 1 BP 23 EP 39 DI 10.1007/s10750-013-1797-x PG 17 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA AC7XD UT WOS:000332745800002 ER PT J AU Li, X Pan, ZL Atungulu, GG Wood, D McHugh, T AF Li, Xuan Pan, Zhongli Atungulu, Griffiths G. Wood, Delilah McHugh, Tara TI Peeling mechanism of tomato under infrared heating: Peel loosening and cracking SO JOURNAL OF FOOD ENGINEERING LA English DT Article DE Infrared radiation; Tomato peeling; Puncture test; Scanning electron microscopy; Stress analysis; Modeling ID FRESH STRAWBERRY PACKAGES; AIR COOLING PROCESS; PROCESSING TOMATOES; QUALITY ATTRIBUTES; AVIAN EGGS; MODEL; STEAM; PEELABILITY; PEACHES; FRUIT AB Critical behaviors of peeling tomatoes using infrared radiation heating are thermally induced peel loosening and subsequent cracking. Fundamental understanding of the two critical behaviors, peel loosening and cracking, remains unclear. This study aimed at investigating the mechanisms of peel separation for tomatoes subjected to a newly developed infrared dry-peeling process. Microstructural changes in tomato epidermal tissues under infrared heating were compared with those of fresh, hot lye and steam treated samples. Theoretical stress analyses coupled with the experimentally measured failure stress of tomato skin were combined to interpret the occurrence of peel cracking within a framework of elastic thin shell theory. With the use of light microscopy and scanning electron microscopy, it was observed that peel loosening due to infrared heating appeared to result from reorganization of extracellular cuticles, thermal expansion of cell walls, and collapse of several cellular layers, differing from samples heated by hot lye and steam. Crack behaviors of tomato skin were attributed to the rapid rate of infrared surface heating which caused the pressure build-up under the skin and strength decrease of the skin. In order to achieve a sufficient skin separation for effective peeling using infrared, promoting rapid and uniform heating on the tomato surface is essential. The findings gained from this study provide new insights for developing the sustainable infrared dry-peeling technology. Published by Elsevier Ltd. C1 [Li, Xuan; Pan, Zhongli; Atungulu, Griffiths G.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Pan, Zhongli; McHugh, Tara] USDA ARS, Western Reg Res Ctr, Proc Foods Res Unit, Albany, CA 94710 USA. [Wood, Delilah] USDA ARS, Western Reg Res Ctr, Bioprod Chem & Engn Res Unit, Albany, CA 94710 USA. RP Pan, ZL (reprint author), USDA ARS, Western Reg Res Ctr, Proc Foods Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM zhongli.pan@ars.usda.gov RI Li, Xuan/J-5786-2015 NR 39 TC 8 Z9 10 U1 7 U2 38 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0260-8774 EI 1873-5770 J9 J FOOD ENG JI J. Food Eng. PD MAY PY 2014 VL 128 BP 79 EP 87 DI 10.1016/j.jfoodeng.2013.12.020 PG 9 WC Engineering, Chemical; Food Science & Technology SC Engineering; Food Science & Technology GA AB5UU UT WOS:000331855000011 ER PT J AU Olanya, OM Ukuku, DO Niemira, BA AF Olanya, O. Modesto Ukuku, Dike O. Niemira, Brendan A. TI Effects of temperatures and storage time on resting populations of Escherichia coli O157:H7 and Pseudomonas fluorescens in vitro SO FOOD CONTROL LA English DT Article DE Temperature; Storage time; Escherichia coli O157:H7; Pseudomonas fluorescens; Interactions ID LISTERIA-MONOCYTOGENES; SALMONELLA-ENTERICA; GROWTH; PATHOGENS; O157-H7; COMPETITION; RHIZOSPHERE; INHIBITION; BIOCONTROL; BACTERIAL AB Assessment of microbial interactions is crucial for documenting bacterial growth in pure and mixed cultures and their potential for biological applications. Pseudomonas fluorescens (non-plant pathogenic and non-pectinolytic) has been used as a biocontrol microbe for plant pathogens and food-borne bacteria. We determined the growth of Escherichia coli O157:H7 (Ec) and P. fluorescens(Pf) in monocultures and co-cultures in sterile distilled water (SDW), buffered peptone water (BPW) and trypticase soy broth (TSB). The effects of temperatures (5, 10, 15, 20, 25, 35, and 37 degrees C) and storage time (0, 2, 4, 6, 24, and 48 h) on bacteria populations were assessed. Bacteria counts in monocultures in SDW ranged from 2.14 to 3.03 and 2.54 to 3.31 Log CFU/ml for Ec and Pf; respectively. In BPW, mean bacteria counts (monocultures) ranged from 3.15 to 6.14 and 2.54 to 6.41 Log CFU/ml for Ec and Pf, respectively. Ec populations in co-culture varied with storage temperatures and time. After 48 h, Ec 43894 monocultures in TSB ranged from 2.17 to 8.75 and 2.31 to 8.85 Log CFU/ml at 20 and 35 degrees C; respectively. In co-cultures with Pf 2-79, Ec 43894 counts ranged from 1.71 to 5.83 (20 degrees C) and 1.90 to 9.03 Log CFU/ml (35 degrees C) in TSB. The reductions of Ec by Pf 2-79 varied among strains and generally ranged from 0.20 to 0.90, 0.63 to 1.18, and 0 to 0.56 Log CFU/ml in BPW (10 degrees C). Substrate availability, storage temperatures, and time significantly (P < 0.05) impacted Ec populations in co-culture. The liquid substrate experiments indicated suppressive conditions of Ec by Pf, however; the reduction of produce contamination by E. coli O157:H7 during transitory temperature abuse conditions such as the transportation of produce from fields needs further investigation. Published by Elsevier Ltd. C1 [Olanya, O. Modesto; Ukuku, Dike O.; Niemira, Brendan A.] ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Olanya, OM (reprint author), ARS, Food Safety & Intervent Technol Res Unit, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM modesto.olanya@ars.usda.gov NR 26 TC 4 Z9 5 U1 0 U2 29 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD MAY PY 2014 VL 39 BP 128 EP 134 DI 10.1016/j.foodcont.2013.11.006 PG 7 WC Food Science & Technology SC Food Science & Technology GA AA9MK UT WOS:000331417400020 ER PT J AU Salgado, SP Pearlstein, AJ Luo, YG Feng, H AF Salgado, Sindy Palma Pearlstein, Arne J. Luo, Yaguang Feng, Hao TI Quality of Iceberg (Lactuca sativa L.) and Romaine (L. sativa L. var. longifolial) lettuce treated by combinations of sanitizer, surfactant, and ultrasound SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Ultrasound; Sanitizer; Surfactant; Quality; Lettuce ID ESCHERICHIA-COLI O157H7; SODIUM DODECYL-SULFATE; SENSORY QUALITY; SHELF-LIFE; STORAGE QUALITY; FRESH PRODUCE; ACID; TEMPERATURE; REDUCTION; EFFICACY AB The effects of sonication, sanitizers and sodium dodecyl sulfate (SDS) on the quality of fresh-cut Iceberg and Romaine lettuce were examined. Lettuce samples were treated for 1 min with and without ultrasound with one of the following solutions: tap water, chlorine, Tsunami, and a combination of Tsunami with 1 g/L SOS. Washed samples were packed under modified atmosphere conditions and stored at 4 degrees C for up to 14 days. Changes in headspace gases, texture, color, tissue damage, visual quality, and natural flora were determined. The O-2 concentrations and CO2 accumulation in Romaine lettuce were not significantly different among the treatments. In Iceberg lettuce, a lower O-2 and high CO2 content in the headspace of samples treated with Tsunami and Tsunami + SDS were recorded. After 14-day storage, the tissue damage expressed by electrolyte leakage, total color difference, firmness, and total aerobic plate counts were not significantly different among treatments in two types of lettuce samples. Treatment of Iceberg lettuce with sonication in combination with Tsunami or Tsunami + SDS did not degrade quality compared to samples treated with chlorine alone, whereas for Romaine lettuce, chlorine-treated samples had a significantly higher overall quality score than that from the other treatments. (C) 2013 Elsevier Ltd. All rights reserved. C1 [Salgado, Sindy Palma; Feng, Hao] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Pearlstein, Arne J.] Univ Illinois, Dept Mech Sci & Engn, Urbana, IL 61801 USA. [Luo, Yaguang] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Feng, H (reprint author), 1304 Penn Ave 382F, Urbana, IL 61801 USA. EM haofeng@illinois.edu OI Pearlstein, Arne/0000-0002-7044-5537 FU United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Initiative [2010-01165] FX This project was supported by United States Department of Agriculture, National Institute of Food and Agriculture, Specialty Crop Initiative (Award No. 2010-01165). NR 29 TC 3 Z9 3 U1 2 U2 48 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD MAY PY 2014 VL 56 IS 2 BP 261 EP 268 DI 10.1016/j.lwt.2013.11.038 PG 8 WC Food Science & Technology SC Food Science & Technology GA AA3WO UT WOS:000331026100007 ER PT J AU White, BL Sanders, TH Davis, JP AF White, Brittany L. Sanders, Timothy H. Davis, Jack P. TI Potential ACE-inhibitory activity and nanoLC-MS/MS sequencing of peptides derived from aflatoxin contaminated peanut meal SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE ACE-inhibitory; Aflatoxin; Bioactive peptides; Peanut meal ID BIOACTIVE PEPTIDES; HYDROLYSIS; REMOVAL; FLOUR; MILK AB Our lab has developed a process for sequestering aflatoxin from contaminated peanut meal (PM) using commercial bentonite clays while protein is simultaneously extracted and hydrolyzed by a commercial protease. The objectives of this study were to sequence generated peptides and evaluate their potential ACE-inhibitory properties. Aflatoxin in the unprocessed PM was 610 mu g kg(-1) compared to 9.71 mu g kg(-1) on a dry weight basis in the 120 min hydrolysate. This hydrolysate displayed significant ACE-inhibitory activity with an IC50 of 295.1 mu g mL(-1). Ultrafiltration and size exclusion chromatography (SEC) improved the ACE-inhibitory properties, with the SEC fraction containing the smallest peptides having an IC50 = 44.4 mu g mL(-1). Additionally, 271 unique peptides were identified by nanoLC-MS/MS, of which 147 belonged to major seed storage proteins. This advanced characterization data will ultimately allow for more efficient production of hydrolysates with ACE-inhibitory activity or other bioactivities of interest from PM. Published by Elsevier Ltd. C1 [White, Brittany L.; Sanders, Timothy H.; Davis, Jack P.] N Carolina State Univ, USDA ARS, Market Qual & Handling Res Unit, Raleigh, NC 27695 USA. [Davis, Jack P.] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. RP White, BL (reprint author), 1114 N Gregg Ave, Fayetteville, AR 72703 USA. EM blwhite001@gmail.com NR 23 TC 3 Z9 3 U1 2 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD MAY PY 2014 VL 56 IS 2 BP 537 EP 542 DI 10.1016/j.lwt.2013.11.039 PG 6 WC Food Science & Technology SC Food Science & Technology GA AA3WO UT WOS:000331026100047 ER PT J AU da Silva, JF AF da Silva, Jessica Fonseca TI Species composition, diversity and structure of novel forests of Castilla elastica in Puerto Rico SO TROPICAL ECOLOGY LA English DT Article DE Basal area; Importance Value Index; native secondary forests; novel forests of Castilla elastica; total aboveground biomass; tree density; species richness; soil bulk density ID TROPICAL FORESTS; INVENTORY DATA; GLOBAL CHANGE; KARST REGION; BIODIVERSITY; RESTORATION; INVASIONS; RECOVERY; BIOMASS AB Attributes of novel forests (secondary forests containing introduced species) were compared with those of native secondary forests of similar age. The study area was the biological reserve called El Tallonal, in Puerto Rico. Species composition, tree density, basal area and soil bulk density were characterized; Importance value index (IVI), Shannon's diversity index (H) and total aboveground biomass (TAGB) were calculated for stands of novel forests for Castilla elastica, an introduced naturalized tree. The IVI of C. elastica was 37 percent; that for Guarea guidonia, a native species, was 14 percent. Compared to native secondary forests, C. elastica forests had similar species richness (27 species in an area of 0.3 ha), low diversity (H' = 0.63) and tree density (1039 trees ha(-1)), moderate basal area (42 m(2) ha(-1)) and TAGB (216 Mg ha(-1)), and low soil bulk density (0.5 to 0.7 g cm(-3)). The structural features of novel forests of C. elastica are in the normal range of variation for those of native secondary forests in the tropics. C1 [da Silva, Jessica Fonseca] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR 00926 USA. [da Silva, Jessica Fonseca] Univ Puerto Rico, Crest Ctr Appl Trop Ecol, San Juan, PR 00931 USA. RP da Silva, JF (reprint author), US Forest Serv, Int Inst Trop Forestry, USDA, 1201 Calle Ceiba,Jardin Bot Sur, Rio Piedras, PR 00926 USA. EM jf483@cam.ac.uk FU International Institute of Tropical Forestry, U.S. Department of Agriculture-Forest Service; Crest-Center for Applied Tropical Ecology and Conservation; University of Puerto Rico FX The funding and technical support provided by the International Institute of Tropical Forestry, U.S. Department of Agriculture-Forest Service, Crest-Center for Applied Tropical Ecology and Conservation and the University of Puerto Rico is greatly acknowledged. I am very grateful to Oscar Abelleira, Humberto Robles, Jaime Diaz and Marcos Caraballo who helped in the field work. I would like to thank Marcos Caraballo who helped in plant identification. I also would like to thank the anonymous reviewers for the valuable comments on the previous version of this manuscript. NR 44 TC 3 Z9 3 U1 1 U2 39 PU INT SOC TROPICAL ECOLOGY PI VARANASI PA DEPT BOTANY BENERAS HINDU UNIV, VARANASI 221 005, INDIA SN 0564-3295 J9 TROP ECOL JI Trop. Ecol. PD MAY PY 2014 VL 55 IS 2 BP 231 EP 244 PG 14 WC Ecology SC Environmental Sciences & Ecology GA AA9RH UT WOS:000331430100008 ER PT J AU Sapozhnikova, Y AF Sapozhnikova, Yelena TI Development of liquid chromatography-tandem mass spectrometry method for analysis of polyphenolic compounds in liquid samples of grape juice, green tea and coffee SO FOOD CHEMISTRY LA English DT Article DE Polyphenolic compounds; LC-MS/MS; Analytical method development; Grape juice; Coffee; Tea ID ANTIOXIDANT ACTIVITY; PHYTOESTROGEN INTAKE; QUANTIFICATION; COMPILATION; PERFORMANCE; FLAVANONES; FLAVONOIDS; BEVERAGES; MEMBRANES; GENISTEIN AB A simple and fast method for the analysis of a wide range of polyphenolic compounds in juice, tea, and coffee samples was developed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method was based on a simple sample preparation "dilute and shoot" approach, and LC-MS/MS quantification using genistein-d(4) as an internal standard. The performance of six different syringeless filter devices was tested for sample preparation. The method was evaluated for recoveries of polyphenols at three spiking levels in juice, tea, and coffee samples. The recoveries of the majority of polyphenols were satisfactory (70-120%), but some varied significantly (20-138%) depending on the matrix. NIST Standard Reference Materials (SRM) 3257 Catechin Calibration Solutions and 3255 Camellia sinensis (Green Tea) Extract with certified concentrations of catechin and epicatechin were used for method validation. The measurement accuracy in two SRMs was 71-113%. The method was successfully applied to the analysis of liquid samples of grape juice, green tea, and coffee. Published by Elsevier Ltd. C1 ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Sapozhnikova, Y (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM yelena.sapozhnikova@ars.usda.gov NR 28 TC 14 Z9 14 U1 5 U2 107 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD MAY 1 PY 2014 VL 150 BP 87 EP 93 DI 10.1016/j.foodchem.2013.10.131 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 295ZU UT WOS:000330155700013 PM 24360423 ER PT J AU Sapozhnikova, Y AF Sapozhnikova, Yelena TI Evaluation of Low-Pressure Gas Chromatography-Tandem Mass Spectrometry Method for the Analysis of > 140 Pesticides in Fish SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE pesticide residues; QuEChERS sample preparation; fast gas chromatography/tandem mass spectrometry; catfish analysis ID ORGANOCHLORINE PESTICIDES; VEGETABLES; RESIDUES; ADIPOSE; TISSUES; FRUITS AB A multiresidue method for the analysis of 143 pesticide residues in fish was developed and evaluated using fast, low-pressure gas chromatography/triple-quadrupole tandem mass spectrometry (LP-GC/MS-MS). The method was based on a QuEChERS (quick, easy, cheap, effective, rugged, safe) extraction with acetonitrile and dispersive solid-phase extraction (d-SPE) cleanup with zirconium-based sorbent. The developed method was evaluated at four spiking levels (1, 5, 50, and 100 ng/g) and further validated by analysis of NIST Standard Reference Materials (SRMs) 197413 and 1947 for selected pesticides with certified concentrations. Acceptable recoveries (70-120%) and standard deviations below 20% were achieved for the majority of pesticides from fortified samples. The measured values for both SRMs agreed with certified values (71-115% accuracy, 4-14% relative standard deviations) for all pesticides, except for p,p-DDD + o,p-DDT (45%) and heptachlor (133%) in SRM 1974b and except for mirex (58%) and trans-chlordane (136%) in SRM 1947. The developed method is fast, simple, and inexpensive with detection limits of 0.5-5 ng/g. Residues of dimethoate, hexachlorobenzene, BHC, lindane, nonachlor, chlorpyrifos, trifluralin, p,p-DDE, p,p-DDD, o,p-DDD, o,p-DDT, p,p-DDD, and chlordane were measured in catfish samples from the market. C1 ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Sapozhnikova, Y (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM yelena.sapozhnikova@ars.usda.gov NR 14 TC 19 Z9 20 U1 2 U2 52 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 30 PY 2014 VL 62 IS 17 BP 3684 EP 3689 DI 10.1021/jf404389e PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AG4DI UT WOS:000335369100005 PM 24387765 ER PT J AU Parry, M Gibson, GJ Parnell, S Gottwald, TR Irey, MS Gast, TC Gilligan, CA AF Parry, Matthew Gibson, Gavin J. Parnell, Stephen Gottwald, Tim R. Irey, Michael S. Gast, Timothy C. Gilligan, Christopher A. TI Bayesian inference for an emerging arboreal epidemic in the presence of control SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE spatiotemporal model; dispersal kernel; stochastic model ID SPATIOTEMPORAL STOCHASTIC-MODELS; INFECTIOUS-DISEASES; PLANT EPIDEMIOLOGY; POPULATIONS; CITRUS; TRANSMISSION; SPREAD; RATES AB The spread of Huanglongbing through citrus groves is used as a case study for modeling an emerging epidemic in the presence of a control. Specifically, the spread of the disease is modeled as a susceptible-exposed-infectious-detected-removed epidemic, where the exposure and infectious times are not observed, detection times are censored, removal times are known, and the disease is spreading through a heterogeneous host population with trees of different age and susceptibility. We show that it is possible to characterize the disease transmission process under these conditions. Two innovations in our work are (i) accounting for control measures via time dependence of the infectious process and (ii) including seasonal and host age effects in the model of the latent period. By estimating parameters in different subregions of a large commercially cultivated orchard, we establish a temporal pattern of invasion, host age dependence of the dispersal parameters, and a close to linear relationship between primary and secondary infectious rates. The model can be used to simulate Huanglongbing epidemics to assess economic costs and potential benefits of putative control scenarios. C1 [Parry, Matthew] Univ Otago, Dept Math & Stat, Dunedin 9054, New Zealand. [Parry, Matthew; Gilligan, Christopher A.] Univ Cambridge, Dept Plant Sci, Epidemiol & Modelling Grp, Cambridge CB2 3EA, England. [Gibson, Gavin J.] Heriot Watt Univ, Dept Actuarial Math & Stat, Edinburgh EH14 4AS, Midlothian, Scotland. [Gibson, Gavin J.] Heriot Watt Univ, Maxwell Inst Math Sci, Edinburgh EH14 4AS, Midlothian, Scotland. [Parnell, Stephen] Rothamsted Res, Dept Computat & Syst Biol, Harpenden AL5 2JQ, Herts, England. [Gottwald, Tim R.] ARS, USDA, Ft Pierce, FL 34945 USA. [Irey, Michael S.; Gast, Timothy C.] US Sugar Corp, Southern Gardens Citrus, Clewiston, FL 33440 USA. RP Gilligan, CA (reprint author), Univ Cambridge, Dept Plant Sci, Epidemiol & Modelling Grp, Cambridge CB2 3EA, England. EM cag1@cam.ac.uk RI parnell, stephen/I-7682-2015 OI parnell, stephen/0000-0002-2625-4557 FU Biotechnology and Biological Sciences Research Council; US Department of Agriculture, Animal and Plant Health Inspection Service, Plant Protection and Quarantine; Citrus Research and Development Foundation FX We thank the many survey inspectors and staff at Southern Gardens Citrus, a subsidiary of US Sugar Corporation, for dedication and contribution to the collection, validation, and summarization of the dataset used in this study. We thank the members of and visitors to the Epidemiology and Modelling Group, Department of Plant Sciences, University of Cambridge. M.P. especially acknowledges many helpful discussions with Joao Filipe, Franco Neri, Erik deSimone, Francisco Laranjeira, Thomas Mang, Richard Stutt, and Nik Cunniffe. C.A.G. gratefully acknowledges the support of a Biotechnology and Biological Sciences Research Council Professorial Fellowship. We also thank the US Department of Agriculture, Animal and Plant Health Inspection Service, Plant Protection and Quarantine, and the Citrus Research and Development Foundation for providing grant funds for the project. NR 22 TC 8 Z9 8 U1 5 U2 28 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 29 PY 2014 VL 111 IS 17 BP 6258 EP 6262 DI 10.1073/pnas.1310997111 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG1TL UT WOS:000335199000046 PM 24711393 ER PT J AU Li, JY Liu, JP Dong, DK Jia, XM McCouch, SR Kochian, LV AF Li, Jian-Yong Liu, Jiping Dong, Dekun Jia, Xiaomin McCouch, Susan R. Kochian, Leon V. TI Natural variation underlies alterations in Nramp aluminum transporter (NRAT1) expression and function that play a key role in rice aluminum tolerance SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE aluminum transport; cell wall aluminum ID FINGER TRANSCRIPTION FACTOR; INTERNAL DETOXIFICATION; ABC TRANSPORTER; CELL-WALLS; GENE; AL; IDENTIFICATION; MATE; INHIBITION; MECHANISMS AB Aluminum (Al) toxicity is a major constraint for crop production on acid soils which compose similar to 40% of arable land in the tropics and subtropics. Rice is the most Al-tolerant cereal crop and offers a good model for identifying Al tolerance genes and mechanisms. Here we investigated natural variation in the rice Nramp aluminum transporter (NRAT1) gene encoding a root plasma membrane Al uptake transporter previously hypothesized to underlie a unique Al tolerance mechanism. DNA sequence variation in the NRAT1 coding and regulatory regions was associated with changes in NRAT1 expression and NRAT1 Al transport properties. These sequence changes resulted in significant differences in Al tolerance that were found to be associated with changes in the Al content of root cell wall and cell sap in 24 representative rice lines from a rice association panel. Expression of the tolerant OsNRAT1 allele in yeast resulted in higher Al uptake than did the sensitive allele and conferred greater Al tolerance when expressed in transgenic Arabidopsis. These findings indicate that NRAT1 plays an important role in rice Al tolerance by reducing the level of toxic Al in the root cell wall and transporting Al into the root cell, where it is ultimately sequestered in the vacuole. Given its ability to enhance Al tolerance in rice and Arabidopsis, this work suggests that the NRAT1 gene or its orthologs may be useful tools for enhancing Al tolerance in a wide range of plant species. C1 [Li, Jian-Yong] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 13853 USA. [Liu, Jiping; Jia, Xiaomin; Kochian, Leon V.] Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Dong, Dekun] Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou, Zhejiang, Peoples R China. [McCouch, Susan R.] Cornell Univ, Dept Plant Breeding, Ithaca, NY 13853 USA. RP Kochian, LV (reprint author), Cornell Univ, ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM Leon.Kochian@ars.usda.gov OI Kochian, Leon/0000-0003-3416-089X FU US Department of Agriculture's Agricultural Food Research Institute [2009-02273] FX We thank Eric Craft, Michael Rutzke, and Shree Giri for conducting the ICP-MS experiments; Adam Famoso for providing rice Al tolerance data; Sandra Harrington for providing rice seeds used in the experiments; and Jon Shaff and Eric Craft for carrying out the rice seed increases in the greenhouse. The work was supported by US Department of Agriculture's Agricultural Food Research Institute Grant 2009-02273. NR 28 TC 21 Z9 22 U1 3 U2 52 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 29 PY 2014 VL 111 IS 17 BP 6503 EP 6508 DI 10.1073/pnas.1318975111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG1TL UT WOS:000335199000087 PM 24728832 ER PT J AU Lee, SG Krishnan, HB Jez, JM AF Lee, Soon Goo Krishnan, Hari B. Jez, Joseph M. TI Structural basis for regulation of rhizobial nodulation and symbiosis gene expression by the regulatory protein NoIR SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE transcription factor; protein structure ID CADC CD(II)/PB(II)/ZN(II)-RESPONSIVE REPRESSOR; SINORHIZOBIUM-FREDII HH103; BRADYRHIZOBIUM-JAPONICUM; SURFACE APPENDAGES; SECRETION SYSTEM; NODABC GENES; ZINC SENSOR; MELILOTI; NODD; NOLR AB The symbiosis between rhizobial microbes and host plants involves the coordinated expression of multiple genes, which leads to nodule formation and nitrogen fixation. As part of the transcriptional machinery for nodulation and symbiosis across a range of Rhizobium, NolR serves as a global regulatory protein. Here, we present the X-ray crystal structures of NolR in the unliganded form and complexed with two different 22-base pair (bp) double-stranded operator sequences (oligos AT and AA). Structural and biochemical analysis of NolR reveals protein-DNA interactions with an asymmetric operator site and defines a mechanism for conformational switching of a key residue (Gln56) to accommodate variation in target DNA sequences from diverse rhizobial genes for nodulation and symbiosis. This conformational switching alters the energetic contributions to DNA binding without changes in affinity for the target sequence. Two possible models for the role of NolR in the regulation of different nodulation and symbiosis genes are proposed. To our knowledge, these studies provide the first structural insight on the regulation of genes involved in the agriculturally and ecologically important symbiosis of microbes and plants that leads to nodule formation and nitrogen fixation. C1 [Lee, Soon Goo; Jez, Joseph M.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Krishnan, Hari B.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Jez, JM (reprint author), Washington Univ, Dept Biol, Campus Box 1137, St Louis, MO 63130 USA. EM jjez@wustl.edu NR 51 TC 4 Z9 4 U1 1 U2 26 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 29 PY 2014 VL 111 IS 17 BP 6509 EP 6514 DI 10.1073/pnas.1402243111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG1TL UT WOS:000335199000088 PM 24733893 ER PT J AU Asters, MC Williams, WP Perkins, AD Mylroie, JE Windham, GL Shan, XY AF Asters, Matthew C. Williams, W. Paul Perkins, Andy D. Mylroie, J. Erik Windham, Gary L. Shan, Xueyan TI Relating significance and relations of differentially expressed genes in response to Aspergillus flavus infection in maize SO SCIENTIFIC REPORTS LA English DT Article ID AFLATOXIN ACCUMULATION; RNA TRANSPORT; INNATE IMMUNITY; NUCLEAR-PORE; RESISTANCE; PCR; ARABIDOPSIS; REGULATORS; EFFECTORS; DYNAMICS AB Aspergillus flavus is a pathogenic fungus infecting maize and producing aflatoxins that are health hazards to humans and animals. Characterizing host defense mechanism and prioritizing candidate resistance genes are important to the development of resistant maize germplasm. We investigated methods amenable for the analysis of the significance and relations among maize candidate genes based on the empirical gene expression data obtained by RT-qPCR technique from maize inbred lines. We optimized a pipeline of analysis tools chosen from various programs to provide rigorous statistical analysis and state of the art data visualization. A network-based method was also explored to construct the empirical gene expression relational structures. Maize genes at the centers in the network were considered as important candidate genes for maize DNA marker studies. The methods in this research can be used to analyze large RT-qPCR datasets and establish complex empirical gene relational structures across multiple experimental conditions. C1 [Asters, Matthew C.; Shan, Xueyan] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Williams, W. Paul; Mylroie, J. Erik; Windham, Gary L.] USDA ARS, Corn Host Plant Resistance Res Unit, Mississippi State, MS USA. [Perkins, Andy D.] Mississippi State Univ, Dept Comp Sci & Engn, Mississippi State, MS 39762 USA. RP Shan, XY (reprint author), Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. EM xshan@bch.msstate.edu FU USDA Agricultural Research Service (ARS) - Specific Cooperative [58-6406-1-600]; USDA-ARS; Mississippi Agri & Forestry Exp Station (MAFES) at Mississippi State University; National Science Foundation [EPS-0903787, EPS-1006883]; Mississippi INBRE; National Center for Research Resources [5P20RR016476-11]; National Institute of General Medical Sciences from the National Institutes of Health [8 P20 GM103476-11] FX This work was supported through the USDA Agricultural Research Service (ARS) funded Specific Cooperative Agreement (No. 58-6406-1-600) between USDA-ARS and Mississippi Agri & Forestry Exp Station (MAFES) at Mississippi State University. ADP was supported by the National Science Foundation under grants EPS-0903787 and EPS-1006883, and by the Mississippi INBRE funded by grants from the National Center for Research Resources (5P20RR016476-11) and the National Institute of General Medical Sciences (8 P20 GM103476-11) from the National Institutes of Health. NR 43 TC 7 Z9 7 U1 0 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD APR 28 PY 2014 VL 4 AR 4815 DI 10.1038/srep04815 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AF8XE UT WOS:000334998100005 PM 24770700 ER PT J AU Tan, JZ Liu, SS Guo, YM Applegate, TJ Eicher, SD AF Tan, Jianzhuang Liu, Shasha Guo, Yuming Applegate, Todd J. Eicher, Susan D. TI Dietary l-arginine supplementation attenuates lipopolysaccharide-induced inflammatory response in broiler chickens SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE TLR4; Broilers; l-Arginine; Lipopolysaccharide ID INFECTIOUS BURSAL DISEASE; NITRIC-OXIDE SYNTHASE; DIFFERENT GENETIC BACKGROUNDS; TOLL-LIKE RECEPTORS; FATTY-ACID RATIO; IMMUNE-RESPONSES; PROINFLAMMATORY CYTOKINES; DIFFERENTIAL EXPRESSION; IMMUNOLOGICAL STRESS; KAPPA-B AB In the present study, two experiments were conducted to investigate the effect of dietary l-arginine (Arg) supplementation on the inflammatory response and innate immunity of broiler chickens. Expt 1 was designed as a 2x3 factorial arrangement (n 8 cages/treatment; n 6 birds/cage) with three dietary Arg concentrations (1 center dot 05, 1 center dot 42 and 1 center dot 90%) and two immune treatments (injection of lipopolysaccharide (LPS) or saline) given at an interval of 48h between 14 and 21d of age. In Expt 2, correlation between dietary Arg concentration (0 center dot 99, 1 center dot 39, 1 center dot 76, 2 center dot 13 or 2 center dot 53%) and percentage of circulating B cells (percentage of circulating lymphocytes) was determined. In Expt 1, LPS injection decreased body-weight gain and feed intake and increased feed conversion ratio of the challenged broilers (14-21d; P<0 center dot 05). LPS injection suppressed (P<0 center dot 05) the percentages of splenic CD11(+) and B cells (percentages of splenic lymphocytes) and phagocytic activity of splenic heterophils and macrophages; Arg supplementation linearly decreased the percentages of CD11(+), CD14(+) and B cells in the spleen (P<0 center dot 10). LPS injection increased (P<0 center dot 05) the expression of IL-1 beta and IL-6 mRNA in the spleen and caecal tonsils. Arginine supplementation decreased (P<0 center dot 05) the expression of IL-1 beta, Toll-like receptor 4 (TLR4) and PPAR-gamma mRNA in the spleen and IL-1 beta, IL-10, TLR4 and NF-kappa B mRNA in the caecal tonsils. In Expt 2, increasing dietary Arg concentrations linearly and quadratically reduced the percentage of circulating B cells (P<0 center dot 01). Collectively, Arg supplementation attenuated the overexpression of pro-inflammatory cytokines probably through the suppression of the TLR4 pathway and CD14(+) cell percentage. Furthermore, excessive Arg supplementation (1 center dot 76%) suppressed the percentages of circulating and splenic B cells. C1 [Tan, Jianzhuang; Liu, Shasha; Guo, Yuming] China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China. [Tan, Jianzhuang; Applegate, Todd J.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Eicher, Susan D.] ARS, Livestock Behav Res Unit, USDA, W Lafayette, IN USA. RP Guo, YM (reprint author), China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China. EM guoyum@cau.edu.cn; applegt@purdue.edu OI Applegate, Todd/0000-0002-2406-5058 FU China Agriculture Research System [CARS-42-G13]; China Scholarship Council FX The present study was supported by a grant from the China Agriculture Research System (CARS-42-G13) and China Scholarship Council. The funders (China Agricultural University and China Scholarship Council) had no role in the design or analysis of the study and in the writing of this article. NR 66 TC 14 Z9 16 U1 5 U2 60 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD APR 28 PY 2014 VL 111 IS 8 BP 1394 EP 1404 DI 10.1017/S0007114513003863 PG 11 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AD9BO UT WOS:000333559700006 PM 24330949 ER PT J AU Short, DPG O'Donnell, K Geiser, DM AF Short, Dylan P. G. O'Donnell, Kerry Geiser, David M. TI Clonality, recombination, and hybridization in the plumbing-inhabiting human pathogen Fusarium keratoplasticum inferred from multilocus sequence typing SO BMC EVOLUTIONARY BIOLOGY LA English DT Article DE Biofilm; Clonality; Fusarium; Hybridization; MLST; Mycotic pathogen; Population dynamics; Recombination ID SOLANI SPECIES COMPLEX; FUNGUS ASPERGILLUS-FUMIGATUS; MOLECULAR MARKERS REVEAL; CONTACT-LENS SOLUTION; CANDIDA-ALBICANS; PHYLOGENETIC DIVERSITY; CRYPTOCOCCUS-NEOFORMANS; GENE GENEALOGIES; HISTOPLASMA-CAPSULATUM; COCCIDIOIDES-IMMITIS AB Background: Recent work has shown that Fusarium species and genotypes most commonly associated with human infections, particularly of the cornea (mycotic keratitis), are the same as those most commonly isolated from plumbing systems. The species most dominant in plumbing biofilms is Fusarium keratoplasticum, a cosmopolitan fungus known almost exclusively from animal infections and biofilms. To better understand its diversity and population dynamics, we developed and utilized a nine-locus sequence-based typing system to make inferences about clonality, recombination, population structure, species boundaries and hybridization. Results: High levels of genetic diversity and evidence for recombination and clonality were detected among 75 clinical and 156 environmental isolates of Fusarium keratoplasticum. The multilocus sequence typing system (MLST) resolved 111 unique nine-locus sequence types (STs). The single locus bifactorial determinants of mating compatibility (mating types MAT1-1 and MAT1-2), were found in a ratio of 70:30. All but one of the 49 isolates of the most common ST (FSSC 2d-2) came from human infections, mostly of the cornea, and from biofilms associated with contact lenses and plumbing surfaces. Significant levels of phylogenetic incongruence were found among loci. Putative clonal relationships among genotypes were estimated, showing a mixture of large clonal complexes and unrelated singletons. Discordance between the nuclear ribosomal rRNA and other gene genealogies is consistent with introgression of ribosomal RNA alleles of phylogenetic species FSSC 9 into F. keratoplasticum. No significant population subdivision based on clinical versus non-clinical sources was found. Conclusions: Incongruent phylogenetic trees and the presence of both mating types within otherwise identical STs were observed, providing evidence for sexuality in F. keratoplasticum. Cryptic speciation suggested in a published three-locus MLST system was not supported with the addition of new loci, but evidence of introgression of ribosomal RNA genes from another strongly supported phylogenetic species (FSSC 9), also known from plumbing systems and human infections, was detected in two isolates. Overall, F. keratoplasticum is a diverse and geographically unstructured species with a mixed clonal and recombinant life history. C1 [Short, Dylan P. G.] Univ Calif Davis, Dept Plant Pathol, Salinas, CA 93905 USA. [O'Donnell, Kerry] USDA ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Geiser, David M.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. RP Short, DPG (reprint author), Univ Calif Davis, Dept Plant Pathol, 1636 E Alisal St, Salinas, CA 93905 USA. EM DGeiser@PSU.edu FU National Science Foundation [DEB 0089474]; National Institute of Food and Agriculture Project at the Pennsylvania State Agricultural Experiment Station [PEN 04527]; Bausch and Lomb, Inc.; NIFA [2010-65110-20488] FX We thank Jean H. Juba for excellent technical assistance. This work was supported in part by a grant from the National Science Foundation, DEB 0089474 to DMG and Gary J. Samuels, National Institute of Food and Agriculture Project PEN 04527 at the Pennsylvania State Agricultural Experiment Station, and by Bausch and Lomb, Inc. DPGS was supported by NIFA grant 2010-65110-20488, Education in Genomics-Based Microbial Forensics. The mention of firm names or trade products does not imply that they are endorsed or recommended by the US Department of Agriculture over other firms or similar products not mentioned. The USDA is an equal opportunity provider and employer. NR 81 TC 8 Z9 10 U1 7 U2 20 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2148 J9 BMC EVOL BIOL JI BMC Evol. Biol. PD APR 26 PY 2014 VL 14 AR 91 DI 10.1186/1471-2148-14-91 PG 14 WC Evolutionary Biology; Genetics & Heredity SC Evolutionary Biology; Genetics & Heredity GA AH3WP UT WOS:000336057200001 PM 24766947 ER PT J AU Weingartl, HM Miller, M Nfon, C Wilson, WC AF Weingartl, Hana M. Miller, Myrna Nfon, Charles Wilson, William C. TI Development of a Rift Valley fever virus viremia challenge model in sheep and goats SO VACCINE LA English DT Article DE Rift Valley fever; Viremia; Virus isolation; Real-time RT-PCR ID AFRICAN DWARF SHEEP; EXPERIMENTAL-INFECTION; DENDRITIC CELLS; DC-SIGN; ALPHAVIRUSES; EFFICACY; ABORTION; MOSQUITO; STRAINS; VACCINE AB Rift Valley fever virus (RVFV), a member of the family Bunyaviridae, causes severe to fatal disease in newborn ruminants, as well as abortions in pregnant animals; both preventable by vaccination. Availability of a challenge model is a pre-requisite for vaccine efficacy trials. Several modes of inoculation with RVFV ZH501 were tested on goats and sheep. Differences in development of infectious viremia were observed between animals inoculated with RVFV produced in mosquito C6/36 cells compared to Vero E6 cell-produced inoculum. Only C6/36-RVFV inoculation led to development of viremia in all inoculated sheep and goats. The C6/36 cell-produced RVFV appeared to be more infectious with earlier onset of viremia, especially in sheep, and may also more closely represent a field situation. Goats were somewhat more resistant to the disease development with lower and shorter infectious virus viremia, and with only some animals developing transient increase in rectal temperature in contrast to sheep. In conclusion, a challenge protocol suitable for goat and sheep vaccine efficacy studies was developed using subcutaneous inoculation of 10(7) PFU per animal with RVFV ZH501 produced in C6/36 cells. Crown Copyright (C) 2014 Published by Elsevier Ltd. C1 [Weingartl, Hana M.; Nfon, Charles] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB R3E 3M4, Canada. [Weingartl, Hana M.] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada. [Miller, Myrna] Univ Wyoming, Laramie, WY 82071 USA. [Wilson, William C.] USDA ARS, Arthropod Borne Anim Dis Res Unit, Manhattan, KS USA. RP Weingartl, HM (reprint author), Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, 1015 Arlington St, Winnipeg, MB R3E 3M4, Canada. EM hana.weingartl@inspection.gc.ca FU CRTI project [06-0138]; CFIA; USDA; ARS CRIS project [5430-32000-005-00D]; U.S. Department of Homeland Security Interagency [HSHQDC-07-X-00982] FX We would like to thank the NML, PHAC and NCFAD, CFIA animal care staff, especially M. Forbes, J. Bernstein, K. Tierney, and C. Nakamura for their help with the animal experiments. The authors would further like to thank S. Zhang, B. Dalman, B. Solylo, E. Weingartl and P. Marszal for the technical assistance. The project was funded in part by a CRTI project RD-06-0138, by CFIA, USDA, ARS CRIS project 5430-32000-005-00D and a U.S. Department of Homeland Security Interagency Agreement HSHQDC-07-X-00982. The contents of this publication are solely responsibility of the authors. NR 30 TC 8 Z9 8 U1 0 U2 3 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD APR 25 PY 2014 VL 32 IS 20 BP 2337 EP 2344 DI 10.1016/j.vaccine.2014.02.066 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA AG0JA UT WOS:000335100600012 PM 24631070 ER PT J AU Weingartl, HM Nfon, CK Zhang, SZ Marszal, P Wilson, WC Morrill, JC Bettinger, GE Peters, CJ AF Weingartl, Hana M. Nfon, Charles K. Zhang, Shunzhen Marszal, Peter Wilson, William C. Morrill, John C. Bettinger, George E. Peters, Clarence J. TI Efficacy of a recombinant Rift Valley fever virus MP-12 with NSm deletion as a vaccine candidate in sheep SO VACCINE LA English DT Article DE Rift Valley fever; Vaccine; NSm deleted MP-12; Sheep ID IMMUNE-RESPONSE; RHESUS MACAQUES; INFECTION; CHALLENGE; PROTEINS; LACKING; SAFETY; IMMUNIZATION; REPLICATION; PROTECTION AB Rift Valley fever virus (RVFV), a mosquito-borne virus in the Bunyaviridae family and Phlebovirus genus, causes RVF, a disease of ruminants and man, endemic in Sub-Saharan African countries. However, outbreaks in Yemen and Saudi Arabia demonstrate the ability for RVFV to spread into virgin territory and thus the need exists to develop safe and efficacious vaccines that can be used outside the endemic zones. Commercial RVFV vaccines are available but have limitations that prevent their use in disease-free countries. Consequently, there are ongoing efforts to develop and/or improve RVFV vaccines with global acceptability. In this study a previously developed MP-12-derived vaccine candidate with a large deletion of the NSm gene in the pre Gn region of the M segment (arMP-12-Delta NSm21/384) developed by T. Ikegami, that was already shown to be safe in pregnant sheep causing neither abortion nor fetal malformation was further evaluated. This vaccine was tested for protection of sheep from viremia and fever following challenge with virulent RVFV ZH501 strain. A single vaccination with arMP-12-Delta NSm21/384 fully protected sheep when challenged four weeks post vaccination, thereby demonstrating that this vaccine is efficacious in protecting these animals from RVFV infection. Crown Copyright (C) 2014 Published by Elsevier Ltd. All rights reserved. C1 [Weingartl, Hana M.; Nfon, Charles K.; Zhang, Shunzhen; Marszal, Peter] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB R3E 3M4, Canada. [Weingartl, Hana M.] Univ Manitoba, Dept Med Microbiol, Winnipeg, MB, Canada. [Wilson, William C.] USDA ARS, ABADRU, Manhattan, KS USA. [Morrill, John C.; Bettinger, George E.; Peters, Clarence J.] UTMB, Galveston, TX USA. RP Nfon, CK (reprint author), Natl Ctr Foreign Anim Dis, 1015 Arlington St, Winnipeg, MB R3E 3M4, Canada. EM charles.nfon@inspection.gc.ca FU CRTI [CRTI-06-0138RD]; US Department of Agriculture, Agricultural Research Service CRIS Project [58-5430-005-00D] FX We would like to thank the staff of the animal care unit for their help with the animal manipulations and monitoring. We also thank Greg Smith for technical assistance and Dr. C. Embury-Hyatt for pathology. This project was funded by CRTI grant, CRTI-06-0138RD. WCW participation was supported by the US Department of Agriculture, Agricultural Research Service CRIS Project # 58-5430-005-00D. NR 28 TC 5 Z9 6 U1 0 U2 5 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD APR 25 PY 2014 VL 32 IS 20 BP 2345 EP 2349 DI 10.1016/j.vaccine.2013.12.064 PG 5 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA AG0JA UT WOS:000335100600013 PM 24462482 ER PT J AU Qin, BL Panickar, KS Anderson, RA AF Qin, Bolin Panickar, Kiran S. Anderson, Richard A. TI Cinnamon polyphenols attenuate the hydrogen peroxide-induced down regulation of S100 beta secretion by regulating sirtuin 1 in C6 rat glioma cells SO LIFE SCIENCES LA English DT Article DE Polyphenols; S100 beta; SIRT1; Oxidative stress ID NF-KAPPA-B; CORTICAL ASTROCYTE CULTURES; IN-VITRO; BIOLOGICAL-ACTIVITY; ALZHEIMERS-DISEASE; INSULIN-RESISTANCE; GLUTAMATE UPTAKE; S100B SECRETION; EXTRACT; RESVERATROL AB Aims: It is well established that the brain is particularly susceptible to oxidative damage due to its high consumption of oxygen. The objective of this study was to investigate the protective effects of a water soluble polyphenol-rich extract of cinnamon and the possible mechanisms, under conditions of oxidative stress-induced by hydrogen peroxide, in rat C6 glioma cells. Main methods: After 24 h of H2O2 incubation, the secretion and intracellular expression of S100 beta were determined by immunoprecitation/immunoblotting and immunofluorescence imaging. Key findings: Cinnamon polyphenols (CP) counteracted the oxidative effects of H2O2 on S100 beta secretion and expression. CP also enhanced the impaired protein levels of sirtuins I, 2, and 3, which are deacetylases important in cell survival. H2O2 also induced the overexpression of the proinflammatory factors, TNF-alpha, phospho-NF-kB p65, as well as of Bcl-xl, Bax and Caspase-3, which are all the members of the Bc1-2 family. CP not only suppressed the expression of these proteins but also attenuated the phosphorylation induced by H2O2. CP also upregulated the decreased Bc1-2 protein levels in H2O2 treated C6 cells. The effects of CP on H2O2-induced downregulation of S100 beta secretion were blocked by SIRTI siRNA demonstrating that SIRT1 plays a regulatory role in CP-mediated prevention by H2O2. Significance: These data demonstrate that Cinnamon polyphenols may exert neuroprotective effects in glial cells by the regulation of BcI-2 family members and enhancing SIRT1 expression during oxidative stress. (c) 2014 Elsevier Inc. All rights reserved. C1 [Qin, Bolin] IN Ingredients Inc, Columbia, TN 38401 USA. [Qin, Bolin; Panickar, Kiran S.; Anderson, Richard A.] USDA ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD 20705 USA. [Panickar, Kiran S.] Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA. RP Qin, BL (reprint author), USDA ARS BHNRC DGIL, Bldg 307C,Rm 215,10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Bolin.Qin@ars.usda.gov FU USDA Cooperative Research and Development Agreement [58-3k95-7-1184]; Integrity Nutraceuticals International, Spring Hill, TN. FX This work was supported in part by a USDA Cooperative Research and Development Agreement (CRADA No. 58-3k95-7-1184) with Integrity Nutraceuticals International, Spring Hill, TN. BQ has a joint appointment with the USDA and IN Ingredients Inc. (formerly Integrity Nutraceuticals, Columbia, TN, USA). The authors would like to thank Dr. Tom Wang for reviewing the manuscript. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 41 TC 1 Z9 1 U1 1 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0024-3205 EI 1879-0631 J9 LIFE SCI JI Life Sci. PD APR 25 PY 2014 VL 102 IS 1 BP 72 EP 79 DI 10.1016/j.lfs.2014.02.038 PG 8 WC Medicine, Research & Experimental; Pharmacology & Pharmacy SC Research & Experimental Medicine; Pharmacology & Pharmacy GA AG0GR UT WOS:000335094400010 PM 24631135 ER PT J AU Feria-Arroyo, TP Castro-Arellano, I Gordillo-Perez, G Cavazos, AL Vargas-Sandoval, M Grover, A Torres, J Medina, RF de Leon, AAP Esteve-Gassent, MD AF Feria-Arroyo, Teresa P. Castro-Arellano, Ivan Gordillo-Perez, Guadalupe Cavazos, Ana L. Vargas-Sandoval, Margarita Grover, Abha Torres, Javier Medina, Raul F. de Leon, Adalberto A. Perez Esteve-Gassent, Maria D. TI Implications of climate change on the distribution of the tick vector Ixodes scapularis and risk for Lyme disease in the Texas-Mexico transboundary region SO PARASITES & VECTORS LA English DT Article DE Ixodes scapularis; Borrelia burgdorferi; Transboundary disease; Lyme disease risk map; Climate change ID BURGDORFERI-SENSU-LATO; EASTERN UNITED-STATES; WHITE-TAILED DEER; BORRELIA-BURGDORFERI; ACARI IXODIDAE; BORNE DISEASES; COMMUNITY COMPOSITION; SPATIAL-DISTRIBUTION; SPECIES DISTRIBUTION; NORTH-AMERICA AB Background: Disease risk maps are important tools that help ascertain the likelihood of exposure to specific infectious agents. Understanding how climate change may affect the suitability of habitats for ticks will improve the accuracy of risk maps of tick-borne pathogen transmission in humans and domestic animal populations. Lyme disease (LD) is the most prevalent arthropod borne disease in the US and Europe. The bacterium Borrelia burgdorferi causes LD and it is transmitted to humans and other mammalian hosts through the bite of infected Ixodes ticks. LD risk maps in the transboundary region between the U.S. and Mexico are lacking. Moreover, none of the published studies that evaluated the effect of climate change in the spatial and temporal distribution of I. scapularis have focused on this region. Methods: The area of study included Texas and a portion of northeast Mexico. This area is referred herein as the Texas-Mexico transboundary region. Tick samples were obtained from various vertebrate hosts in the region under study. Ticks identified as I. scapularis were processed to obtain DNA and to determine if they were infected with B. burgdorferi using PCR. A maximum entropy approach (MAXENT) was used to forecast the present and future (2050) distribution of B. burgdorferi-infected I. scapularis in the Texas-Mexico transboundary region by correlating geographic data with climatic variables. Results: Of the 1235 tick samples collected, 109 were identified as I. scapularis. Infection with B. burgdorferi was detected in 45% of the I. scapularis ticks collected. The model presented here indicates a wide distribution for I. scapularis, with higher probability of occurrence along the Gulf of Mexico coast. Results of the modeling approach applied predict that habitat suitable for the distribution of I. scapularis in the Texas-Mexico transboundary region will remain relatively stable until 2050. Conclusions: The Texas-Mexico transboundary region appears to be part of a continuum in the pathogenic landscape of LD. Forecasting based on climate trends provides a tool to adapt strategies in the near future to mitigate the impact of LD related to its distribution and risk for transmission to human populations in the Mexico-US transboundary region. C1 [Feria-Arroyo, Teresa P.; Cavazos, Ana L.] Univ Texas Pan Amer, Dept Biol, Edinburg, TX 78539 USA. [Castro-Arellano, Ivan] Texas State Univ, Dept Biol, Coll Sci & Engn, San Marcos, TX 78666 USA. [Gordillo-Perez, Guadalupe; Torres, Javier] IMSS, Unidad Invest Enfermedades Infecciosas, Ctr Med Nacl SXXI, Mexico City 06720, DF, Mexico. [Vargas-Sandoval, Margarita] Univ Michoacana, Fac Agrobiol, Uruapan 60090, Michoacan, Mexico. [Grover, Abha; Esteve-Gassent, Maria D.] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. [Medina, Raul F.] Texas A&M Univ, Coll Agr & Life Sci, Dept Entomol, College Stn, TX 77843 USA. [de Leon, Adalberto A. Perez] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. RP Esteve-Gassent, MD (reprint author), Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Pathobiol, College Stn, TX 77843 USA. EM Mesteve-Gassent@cvm.tamu.edu FU Department of Veterinary Pathobiology, Texas AM University [TAMU-CONACYT-052]; Texas State University; Fundacion IMSS, Mexico FX Authors want to thank the Department of Texas Parks and Wildlife for their help providing tick samples from a number of wildlife management areas. Also authors would like to thank Veterinary clinics across the state of Texas, and the Brazos Valley animal shelter (currently Aggieland Humane Society) and Drs. Thomas Craig and Karen Snowden for their help collecting ticks for this study. USDA is an equal opportunity provider and employer. Support for this study came from the Department of Veterinary Pathobiology, Texas A&M University, the AgriLife-TVMDL seed grant to the project entitled "Improving diagnostic methods for Lyme disease, and epidemiology of human and animal infections in TX" and the bi-national cooperative grant TAMU-CONACYT-052 'Typing virulent isolates of Lyme disease agents in central Mexico' to MDEG. CONACYT-SALUD 1999-30694-M and 2008-87686. ICA received a Research Enhancement Program Grant from Texas State University. JT is a recipient of an exclusivity scholarship from Fundacion IMSS, Mexico. NR 116 TC 15 Z9 18 U1 7 U2 72 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD APR 25 PY 2014 VL 7 AR 199 DI 10.1186/1756-3305-7-199 PG 16 WC Parasitology SC Parasitology GA AG3TY UT WOS:000335343900002 PM 24766735 ER PT J AU Solaiman, DKY Liu, YH Moreau, RA Zerkowski, JA AF Solaiman, Daniel K. Y. Liu, Yanhong Moreau, Robert A. Zerkowski, Jonathan A. TI Cloning, characterization, and heterologous expression of a novel glucosyltransferase gene from sophorolipid-producing Candida bombicola SO GENE LA English DT Article DE Candida bombicola; gtf-1; Glucosyltransferase; Sophorolipid; Sterol ID ACID SOPHOROSIDE PRODUCTION; GROWTH-MEDIUM; FATTY-ACIDS; BOGORIENSIS; GLUCOSE; BIOSYNTHESIS; PHYTOSTEROLS; GLYCOLIPIDS; PCR AB Candida bombicola is well-studied for the production of a biosurfactant, the sophorolipids. In this paper, the cloning of a glucosyltransferase gene using polymerase-chain-reaction (PCR) technique is described. Degenerative primer-pairs were first designed based on the highly conserved amino-acid sequences of several selected yeast glucosyltransferases. Using these primers, an amplified sequence (amplicon) of 700 base-pair from C bombicola was obtained and subsequently sequenced. Based on the sequence of this amplicon, additional target-specific PCR primers were designed for use in subsequent rounds of 3'- and 5'-extension using DNA walking technique to eventually obtain a C. bombicola genomic sequence containing an open-reading-frame putatively identified as a glucosyltransferase (gtf-1). The gene was subcloned in Saccharomyces cerevisiae for expression and functional characterization. Quantitative RT-PCR confirmed the expression of gtf-1 in the recombinant S. cerevisiae. In vitro assay with the sonicated cells of the recombinant yeast confirms the presence of glucosylation activity on sterol and hydroxy fatty add substrates. This study reports for the first time the cloning and characterization of a broad-specificity lipid glucosylation gene from C. bombicola, and the functional activity of its gene product. Published by Elsevier B.V. C1 [Solaiman, Daniel K. Y.; Zerkowski, Jonathan A.] ARS, Biobased & Other Anim Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Liu, Yanhong] ARS, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Moreau, Robert A.] ARS, Sustainable Biofuels & Coprod Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Solaiman, DKY (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM dan.solaiman@ars.usda.gov OI Moreau, Robert/0000-0002-8166-8322 NR 39 TC 2 Z9 2 U1 2 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD APR 25 PY 2014 VL 540 IS 1 BP 46 EP 53 DI 10.1016/j.gene.2014.02.029 PG 8 WC Genetics & Heredity SC Genetics & Heredity GA AE6XJ UT WOS:000334141000008 PM 24566005 ER PT J AU Borer, ET Seabloom, EW Gruner, DS Harpole, WS Hillebrand, H Lind, EM Adler, PB Alberti, J Anderson, TM Bakker, JD Biederman, L Blumenthal, D Brown, CS Brudvig, LA Buckley, YM Cadotte, M Chu, CJ Cleland, EE Crawley, MJ Daleo, P Damschen, EI Davies, KF DeCrappeo, NM Du, GZ Firn, J Hautier, Y Heckman, RW Hector, A HilleRisLambers, J Iribarne, O Klein, JA Knops, JMH La Pierre, KJ Leakey, ADB Li, W MacDougall, AS McCulley, RL Melbourne, BA Mitchell, CE Moore, JL Mortensen, B O'Halloran, LR Orrock, JL Pascual, J Prober, SM Pyke, DA Risch, AC Schuetz, M Smith, MD Stevens, CJ Sullivan, LL Williams, RJ Wragg, PD Wright, JP Yang, LH AF Borer, Elizabeth T. Seabloom, Eric W. Gruner, Daniel S. Harpole, W. Stanley Hillebrand, Helmut Lind, Eric M. Adler, Peter B. Alberti, Juan Anderson, T. Michael Bakker, Jonathan D. Biederman, Lori Blumenthal, Dana Brown, Cynthia S. Brudvig, Lars A. Buckley, Yvonne M. Cadotte, Marc Chu, Chengjin Cleland, Elsa E. Crawley, Michael J. Daleo, Pedro Damschen, Ellen I. Davies, Kendi F. DeCrappeo, Nicole M. Du, Guozhen Firn, Jennifer Hautier, Yann Heckman, Robert W. Hector, Andy HilleRisLambers, Janneke Iribarne, Oscar Klein, Julia A. Knops, Johannes M. H. La Pierre, Kimberly J. Leakey, Andrew D. B. Li, Wei MacDougall, Andrew S. McCulley, Rebecca L. Melbourne, Brett A. Mitchell, Charles E. Moore, Joslin L. Mortensen, Brent O'Halloran, Lydia R. Orrock, John L. Pascual, Jesus Prober, Suzanne M. Pyke, David A. Risch, Anita C. Schuetz, Martin Smith, Melinda D. Stevens, Carly J. Sullivan, Lauren L. Williams, Ryan J. Wragg, Peter D. Wright, Justin P. Yang, Louie H. TI Herbivores and nutrients control grassland plant diversity via light limitation SO NATURE LA English DT Article ID SPECIES-DIVERSITY; RESOURCE CONTROL; COMPETITION; GROWTH; ENVIRONMENTS; VEGETATION; CONSUMER; DEFENSE; DEPENDS; IMPACT AB Human alterations to nutrient cycles(1,2) and herbivore communities(3-7) are affecting global biodiversity dramatically(2). Ecological theory predicts these changes should be strongly counteractive: nutrient addition drives plant species loss through intensified competition for light, whereas herbivores prevent competitive exclusion by increasing ground-level light, particularly in productive systems(8,9). Here we use experimental data spanning a globally relevant range of conditions to test the hypothesis that herbaceous plant species losses caused by eutrophication may be offset by increased light availability due to herbivory. This experiment, replicated in 40 grasslands on 6 continents, demonstrates that nutrients and herbivores can serve as counteracting forces to control local plant diversity through light limitation, independent of site productivity, soil nitrogen, herbivore type and climate. Nutrient addition consistently reduced local diversity through light limitation, and herbivory rescued diversity at sites where it alleviated light limitation. Thus, species loss from anthropogenic eutrophication can be ameliorated in grasslands where herbivory increases ground-level light. C1 [Borer, Elizabeth T.; Seabloom, Eric W.; Lind, Eric M.; Hautier, Yann; Wragg, Peter D.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Gruner, Daniel S.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Harpole, W. Stanley; Biederman, Lori; Li, Wei; Mortensen, Brent; Sullivan, Lauren L.; Williams, Ryan J.] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Hillebrand, Helmut] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm, D-26382 Oldenburg, Germany. [Adler, Peter B.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Adler, Peter B.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Alberti, Juan; Daleo, Pedro; Iribarne, Oscar; Pascual, Jesus] Consejo Nacl Invest Cient & Tecn, IIMyC, RA-7600 Mar Del Plata, Argentina. [Anderson, T. Michael] Wake Forest Univ, Dept Biol, Winston Salem, NC 27109 USA. [Bakker, Jonathan D.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Blumenthal, Dana] ARS, USDA, Ft Collins, CO 80526 USA. [Brown, Cynthia S.; Klein, Julia A.; Smith, Melinda D.] Colorado State Univ, Dept Forest Rangeland & Watershed Stewardship, Ft Collins, CO 80523 USA. [Brudvig, Lars A.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Buckley, Yvonne M.] Univ Queensland, Sch Biol Sci, ARC Ctr Excellence Environm Decis, Brisbane, Qld 4072, Australia. [Buckley, Yvonne M.] Trinity Coll Dublin, Sch Nat Sci, Dublin 2, Ireland. [Cadotte, Marc] Univ Toronto, Dept Ecol & Evolutionary Biol, Scarborough, ON M1C 1A4, Canada. [Chu, Chengjin; Du, Guozhen] Lanzhou Univ, Sch Life Sci, Res Stn Alpine Meadow & Wetland Ecosyst, State Key Lab Grassland & Agroecosyst, Lanzhou 730000, Gansu, Peoples R China. [Cleland, Elsa E.] Univ Calif San Diego, Div Biol Sci, San Diego, CA 92093 USA. [Crawley, Michael J.] Univ London Imperial Coll Sci Technol & Med, Dept Biol, Ascot SL5 7PY, Berks, England. [Damschen, Ellen I.; Orrock, John L.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Davies, Kendi F.; Melbourne, Brett A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [DeCrappeo, Nicole M.; Pyke, David A.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. [Firn, Jennifer] Queensland Univ Technol, Brisbane, Qld 4001, Australia. [Heckman, Robert W.; Mitchell, Charles E.] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA. [Hector, Andy] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England. [HilleRisLambers, Janneke] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Knops, Johannes M. H.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [La Pierre, Kimberly J.] Univ Calif Berkeley, Berkeley Initiat Global Change Biol, Berkeley, CA 94704 USA. [Leakey, Andrew D. B.] Univ Illinois, Dept Plant Biol, Champaign, IL 61820 USA. [MacDougall, Andrew S.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [McCulley, Rebecca L.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Moore, Joslin L.] Univ Melbourne, Sch Bot, Australian Res Ctr Urban Ecol, Melbourne, Vic 3010, Australia. [Moore, Joslin L.] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia. [O'Halloran, Lydia R.] Oregon State Univ, Dept Zool, Corvallis, OR 97331 USA. [Prober, Suzanne M.] CSIRO Ecosyst Sci, Wembley, WA 6913, Australia. [Risch, Anita C.; Schuetz, Martin] Swiss Fed Inst Forest Snow & Landscape Res, CH-8903 Birmensdorf, Switzerland. [Stevens, Carly J.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Wright, Justin P.] Duke Univ, Dept Biol, Durham, NC 27708 USA. [Yang, Louie H.] Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA. RP Borer, ET (reprint author), Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. EM borer@umn.edu RI Risch, Anita/A-9836-2012; Leakey, Andrew/Q-9889-2016; Prober, Suzanne/G-6465-2010; Adler, Peter/D-3781-2009; Heckman, Robert/J-2640-2015; Hector, Andrew/H-4199-2011; MacDougall, Andrew/F-2037-2011; Gruner, Daniel/A-5166-2010; Mitchell, Charles/I-3709-2014; Buckley, Yvonne/B-1281-2008; Smith, Melinda/J-8987-2014; Harpole, William/C-2814-2013; Hillebrand, Helmut/I-1717-2014; Moore, Joslin/C-5270-2009; Hautier, Yann/D-5426-2015 OI Biederman, Lori/0000-0003-2171-7898; Lind, Eric/0000-0003-3051-7724; Risch, Anita/0000-0003-0531-8336; Leakey, Andrew/0000-0001-6251-024X; Firn, Jennifer/0000-0001-6026-8912; Seabloom, Eric/0000-0001-6780-9259; Daleo, Pedro/0000-0001-9759-1203; Borer, Elizabeth/0000-0003-2259-5853; La Pierre, Kimberly/0000-0001-7056-4547; Heckman, Robert/0000-0002-2281-3091; Hector, Andrew/0000-0002-1309-7716; Gruner, Daniel/0000-0002-3153-4297; Mitchell, Charles/0000-0002-1633-1993; Buckley, Yvonne/0000-0001-7599-3201; Harpole, William/0000-0002-3404-9174; Hillebrand, Helmut/0000-0001-7449-1613; Moore, Joslin/0000-0001-9809-5092; Hautier, Yann/0000-0003-4347-7741 FU NSF Research Coordination Network [NSF-DEB-1042132]; NSF Long Term Ecological Research program [NSF-DEB-1234162]; UMN Institute on the Environment [DG-0001-13] FX This work uses data from the Nutrient Network (http://nutnet.org) experiment, funded at the site scale by individual researchers. Coordination and data management are supported by funding to E. Borer and E. Seabloom from the NSF Research Coordination Network (NSF-DEB-1042132) and Long Term Ecological Research (NSF-DEB-1234162 to Cedar Creek LTER) programs and the UMN Institute on the Environment (DG-0001-13). The Minnesota Supercomputer Institute hosts project data. We are grateful to F. Isbell for suggestions that improved the manuscript. Any use of trade names is for descriptive purposes only and does not imply endorsement by the US Government. NR 31 TC 88 Z9 95 U1 55 U2 425 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD APR 24 PY 2014 VL 508 IS 7497 BP 517 EP + DI 10.1038/nature13144 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AF5GI UT WOS:000334741600035 PM 24670649 ER PT J AU Hautier, Y Seabloom, EW Borer, ET Adler, PB Harpole, WS Hillebrand, H Lind, EM MacDougall, AS Stevens, CJ Bakker, JD Buckley, YM Chu, CJ Collins, SL Daleo, P Damschen, EI Davies, KF Fay, PA Firn, J Gruner, DS Jin, VL Klein, JA Knops, JMH La Pierre, KJ Li, W McCulley, RL Melbourne, BA Moore, JL O'Halloran, LR Prober, SM Risch, AC Sankaran, M Schuetz, M Hector, A AF Hautier, Yann Seabloom, Eric W. Borer, Elizabeth T. Adler, Peter B. Harpole, W. Stanley Hillebrand, Helmut Lind, Eric M. MacDougall, Andrew S. Stevens, Carly J. Bakker, Jonathan D. Buckley, Yvonne M. Chu, Chengjin Collins, Scott L. Daleo, Pedro Damschen, Ellen I. Davies, Kendi F. Fay, Philip A. Firn, Jennifer Gruner, Daniel S. Jin, Virginia L. Klein, Julia A. Knops, Johannes M. H. La Pierre, Kimberly J. Li, Wei McCulley, Rebecca L. Melbourne, Brett A. Moore, Joslin L. O'Halloran, Lydia R. Prober, Suzanne M. Risch, Anita C. Sankaran, Mahesh Schuetz, Martin Hector, Andy TI Eutrophication weakens stabilizing effects of diversity in natural grasslands SO NATURE LA English DT Article ID ECOSYSTEM STABILITY; PLANT DIVERSITY; ECOLOGICAL COMMUNITIES; EUROPEAN GRASSLANDS; NITROGEN DEPOSITION; BIODIVERSITY LOSS; SPECIES RICHNESS; PRODUCTIVITY; LOSSES; POPULATION AB Studies of experimental grassland communities(1-7) have demonstrated that plant diversity can stabilize productivity through species asynchrony, in which decreases in the biomass of some species are compensated for by increases in others(1,2). However, it remains unknown whether these findings are relevant to natural ecosystems, especially those for which species diversity is threatened by anthropogenic global change(8-11). Here we analyse diversity-stability relationships from 41 grasslands on five continents and examine how these relationships are affected by chronic fertilization, one of the strongest drivers of species loss globally(8). Unmanipulated communities with more species had greater species asynchrony, resulting in more stable biomass production, generalizing a result from biodiversity experiments to real-world grasslands. However, fertilization weakened the positive effect of diversity on stability. Contrary to expectations, this was not due to species loss after eutrophication but rather to an increase in the temporal variation of productivity in combination with a decrease in species asynchrony in diverse communities. Our results demonstrate separate and synergistic effects of diversity and eutrophication on stability, emphasizing the need to understand how drivers of global change interactively affect the reliable provisioning of ecosystem services in real-world systems. C1 [Hautier, Yann; Seabloom, Eric W.; Borer, Elizabeth T.; Lind, Eric M.] Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. [Hautier, Yann] Univ Zurich, Inst Evolutionary Biol & Environm Studies, CH-8057 Zurich, Switzerland. [Adler, Peter B.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Adler, Peter B.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Harpole, W. Stanley] Iowa State Univ, Dept Ecol Evolut & Organismal Biol, Ames, IA 50011 USA. [Hillebrand, Helmut] Carl von Ossietzky Univ Oldenburg, Inst Chem & Biol Marine Environm, D-26111 Oldenburg, Germany. [MacDougall, Andrew S.] Univ Guelph, Dept Integrat Biol, Guelph, ON N1G 2W1, Canada. [Stevens, Carly J.] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Bakker, Jonathan D.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Buckley, Yvonne M.] Univ Queensland, Sch Biol Sci, Australian Res Council Ctr Excellence Environm De, Brisbane, Qld 4072, Australia. [Buckley, Yvonne M.] Trinity Coll Dublin, Dept Zool, Sch Nat Sci, Dublin 2, Ireland. [Chu, Chengjin] Lanzhou Univ, Sch Life Sci, Res Stn Alpine Meadow & Wetland Ecosyst, State Key Lab Grassland & Agroecosyst, Lanzhou 730000, Peoples R China. [Collins, Scott L.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Daleo, Pedro] UNMdP, CONICET, IIMyC, RA-7600 Mar Del Plata, Argentina. [Damschen, Ellen I.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Damschen, Ellen I.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Davies, Kendi F.; Melbourne, Brett A.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Fay, Philip A.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Firn, Jennifer] Queensland Univ Technol, Sch Biol Sci, Brisbane, Qld 4000, Australia. [Gruner, Daniel S.] Univ Maryland, Dept Entomol, College Pk, MD 20742 USA. [Jin, Virginia L.] USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE 68583 USA. [Klein, Julia A.] Colorado State Univ, Dept Forest Rangeland & Watershed Stewardship, Ft Collins, CO 80523 USA. [Knops, Johannes M. H.] Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA. [La Pierre, Kimberly J.] Univ Calif Berkeley, Berkeley Initiat Global Change Biol, Berkeley, CA 94720 USA. [Li, Wei] Southwest Forestry Univ, Yunnan Acad Biodivers, Kunming 650224, Peoples R China. [McCulley, Rebecca L.] Univ Kentucky, Dept Plant & Soil Sci, Lexington, KY 40546 USA. [Moore, Joslin L.] Univ Melbourne, Sch Bot, Australian Res Ctr Urban Ecol, Melbourne, Vic 3010, Australia. [Moore, Joslin L.] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia. [O'Halloran, Lydia R.] Oregon State Univ, Dept Zool, Corvallis, OR 97331 USA. [Prober, Suzanne M.] CSIRO Ecosyst Sci, Wembley, WA 6913, Australia. [Risch, Anita C.; Schuetz, Martin] Swiss Fed Inst Forest Snow & Landscape Res, CH-8903 Birmensdorf, Switzerland. [Sankaran, Mahesh] Univ Leeds, Sch Biol, Leeds LS2 9JT, W Yorkshire, England. [Sankaran, Mahesh] Natl Ctr Biol Sci, Bangalore 560065, Karnataka, India. [Hector, Andy] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England. RP Hautier, Y (reprint author), Univ Minnesota, Dept Ecol Evolut & Behav, St Paul, MN 55108 USA. EM hauti001@umn.edu RI MacDougall, Andrew/F-2037-2011; Gruner, Daniel/A-5166-2010; Buckley, Yvonne/B-1281-2008; Harpole, William/C-2814-2013; Hillebrand, Helmut/I-1717-2014; Moore, Joslin/C-5270-2009; Hautier, Yann/D-5426-2015; Adler, Peter/D-3781-2009; Hector, Andrew/H-4199-2011; Risch, Anita/A-9836-2012; Collins, Scott/P-7742-2014; Prober, Suzanne/G-6465-2010 OI Firn, Jennifer/0000-0001-6026-8912; Seabloom, Eric/0000-0001-6780-9259; Daleo, Pedro/0000-0001-9759-1203; Borer, Elizabeth/0000-0003-2259-5853; La Pierre, Kimberly/0000-0001-7056-4547; Lind, Eric/0000-0003-3051-7724; Fay, Philip/0000-0002-8291-6316; Gruner, Daniel/0000-0002-3153-4297; Buckley, Yvonne/0000-0001-7599-3201; Harpole, William/0000-0002-3404-9174; Hillebrand, Helmut/0000-0001-7449-1613; Moore, Joslin/0000-0001-9809-5092; Hautier, Yann/0000-0003-4347-7741; Hector, Andrew/0000-0002-1309-7716; Risch, Anita/0000-0003-0531-8336; Collins, Scott/0000-0002-0193-2892; FU European Union [298935]; National Science Foundation Research Coordination Network [NSF-DEB-1042132]; Long Term Ecological Research (LTER) programme [NSF-DEB-1234162]; Institute on the Environment at the University of Minnesota [DG-0001-13] FX The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 298935 to Y.H. (with A. H. and E. W. S.). This work was generated using data from the Nutrient Network (http://www.nutnet.org) experiment, funded at the site-scale by individual researchers. Coordination and data management have been supported by funding to E. T. B. and E. W. S. from the National Science Foundation Research Coordination Network (NSF-DEB-1042132), the Long Term Ecological Research (LTER) programme (NSF-DEB-1234162 to Cedar Creek as well as other LTER sites), and the Institute on the Environment at the University of Minnesota (DG-0001-13). We also thank the Minnesota Supercomputer Institute for hosting project data, and the Institute on the Environment for hosting Network meetings. We thank R. S. L. Veiga, F. Isbell, R. K. Didham, H. McGinness and M. O'Brien for suggestions that improved the manuscript. NR 42 TC 46 Z9 49 U1 39 U2 306 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0028-0836 EI 1476-4687 J9 NATURE JI Nature PD APR 24 PY 2014 VL 508 IS 7497 BP 521 EP + DI 10.1038/nature13014 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AF5GI UT WOS:000334741600036 PM 24531763 ER PT J AU Nicklas, TA O'Neil, CE Fulgoni, VL AF Nicklas, Theresa A. O'Neil, Carol E. Fulgoni, Victor L., III TI Snacking patterns, diet quality, and cardiovascular risk factors in adults SO BMC PUBLIC HEALTH LA English DT Article DE Adults; Snacking patterns; Overweight; Abdominal obesity; Cardiovascular risk factors; Diet quality ID BODY-MASS INDEX; NUTRITION EXAMINATION SURVEY; HEALTHY EATING INDEX-2005; 4 AGE-GROUPS; METABOLIC SYNDROME; FOOD-INTAKE; ABDOMINAL OBESITY; NATIONAL-HEALTH; WEIGHT STATUS; ENERGY-INTAKE AB Background: The relationship of snacking patterns on nutrient intake and cardiovascular risk factors (CVRF) in adults is unknown. The aim of this study was to examine the associations of snacking patterns with nutrient intake, diet quality, and a selection of CVRF in adults participating in the 2001-2008 National Health and Nutrition Examination Survey. Methods: 24-hour dietary recalls were used to determine intake and cluster analysis was used to identify the snacking patterns. Height and weight were obtained and the health indices that were evaluated included diastolic and systolic blood pressure, high density lipoprotein-cholesterol, low density lipoprotein cholesterol, triacylglycerides, blood glucose, and insulin. Results: The sample was participants (n = 18,988) 19+ years (50% males; 11% African-Americans; 72% white, 12% Hispanic-Americans, and 5% other). Cluster analyses generated 12 distinct snacking patterns, explaining 61% of the variance in snacking. Comparisons of snacking patterns were made to the no snack pattern. It was found that miscellaneous snacks constituted the most common snacking pattern (17%) followed by cakes/cookies/pastries (12%) and sweets (9%). Most snacking patterns were associated with higher energy intakes. Snacking patterns cakes/cookies/pastries, vegetables/legumes, crackers/salty snacks, other grains and whole fruit were associated with lower intakes of saturated fatty acids. Added sugars intakes were higher in the cakes/cookies/pastries, sweets, milk desserts, and soft drinks patterns. Five snack patterns (cakes/cookies/pastries, sweets, vegetable/legumes, milk desserts, soft drinks) were associated with lower sodium intakes. Several snack patterns were associated with higher intakes of potassium, calcium, fiber, vitamin A, and magnesium. Five snacking patterns (miscellaneous snacks; vegetables/legumes; crackers/salty snacks; other grains; and whole fruit) were associated with better diet quality scores. Alcohol was associated with a lower body mass index and milk desserts were associated with a lower waist circumference. No snack patterns were associated with other CVRF studied. Conclusions: Overall, several snacking patterns were associated with better diet quality than those consuming no snacks. Yet, the majority of the snacking patterns were not associated with CVRF. Education is needed to improve snacking patterns in terms of nutrients to limit in the diet along with more nutrient-dense foods to be included in snacks. C1 [Nicklas, Theresa A.] USDA ARS, Dept Pediat, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [O'Neil, Carol E.] Louisiana State Univ, Ctr Agr, Sch Nutr & Food Sci, Baton Rouge, LA 70803 USA. [Fulgoni, Victor L., III] Nutr Impact LLC, Battle Creek, MI 49014 USA. RP Nicklas, TA (reprint author), USDA ARS, Dept Pediat, Baylor Coll Med, Childrens Nutr Res Ctr, 1100 Bates Ave, Houston, TX 77030 USA. EM tnicklas@bcm.edu FU USDA Agricultural Research Service [58-6250-0-008]; USDA Hatch Project [LAB 93951]; Kellogg's Corporate Citizenship Fund FX This work is a publication of the United States Department of Agriculture (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, and Houston, Texas. The contents of this publication do not necessarily reflect the views or policies of the USDA, nor does mention of trade names, commercial products, or organizations imply endorsement from the U.S. government. This research project was supported by the USDA Agricultural Research Service through specific cooperative agreement 58-6250-0-008. Partial support was received from the USDA Hatch Project LAB 93951 and the Kellogg's Corporate Citizenship Fund. The funding agencies had no input into the study design or interpretation of the data. Special thanks to Lori Briones for help in preparing the manuscript and Bee Wong for obtaining research articles. NR 84 TC 10 Z9 11 U1 1 U2 24 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD APR 23 PY 2014 VL 14 AR 388 DI 10.1186/1471-2458-14-388 PG 14 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AH5OH UT WOS:000336179100002 PM 24754905 ER PT J AU Fuller, SA Farmer, BD Beck, BH AF Fuller, S. Adam Farmer, Bradley D. Beck, Benjamin H. TI White bass Morone chrysops is less susceptible than its hybrid to experimental infection with Flavobacterium columnare SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Columnaris disease; Hybrid striped bass; Dose response; Differential survival ID CATFISH ICTALURUS-PUNCTATUS; SUNSHINE BASS; STRIPED BASS; FLEXIBACTER-COLUMNARIS; STRESS-RESPONSE; SAXATILIS MALE; DISEASE; ADHESION; RESISTANCE; CHALLENGE AB Hybrid striped bass (HSB) and white bass (WB) were evaluated for their susceptibility to Flavobacterium columnare, the causative agent of columnaris disease, in 3 fundamental studies. In the first experiment, we determined whether columnaris disease could be developed by experimental challenge in HSB. This challenge consisted of 3 levels of F. columnare (10, 30, and 60 ml volumes) determined to be 2.25 x 10(7), 6.75 x 10(7), and 1.35 x 10(8) CFU ml(-1), respectively. Each treatment group exhibited significantly different survival rates: 0, 3.3, and 13.3% in the 60, 30, and 10 ml groups, respectively. In Expt 2, using the 30 ml dose, both HSB and WB had a 0% survival rate, with WB taking significantly longer to reach 100% mortality. In Expt 3, using the 10 ml dose, no HSB survived, whereas 33% of WB survived (p < 0.0001). Compared to controls, HSB treated with 10 ml showed extensive gill damage at 24 h, which could have contributed to the higher mortality observed in HSB; in contrast, WB gills showed noticeably less damage. From these series of experiments, it is clear that HSB are more sensitive to F. columnare, having lower survival and more extensive histological damage compared to WB following challenge. C1 [Fuller, S. Adam; Farmer, Bradley D.; Beck, Benjamin H.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Fuller, SA (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, POB 1050, Stuttgart, AR 72160 USA. EM adam.fuller@ars.usda.gov NR 30 TC 1 Z9 1 U1 1 U2 7 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD APR 23 PY 2014 VL 109 IS 1 BP 15 EP 22 DI 10.3354/dao02716 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA AG7EG UT WOS:000335580300003 PM 24781793 ER PT J AU Goettel, W Xia, E Upchurch, R Wang, ML Chen, PY An, YQC AF Goettel, Wolfgang Xia, Eric Upchurch, Robert Wang, Ming-Li Chen, Pengyin An, Yong-Qiang Charles TI Identification and characterization of transcript polymorphisms in soybean lines varying in oil composition and content SO BMC GENOMICS LA English DT Article ID SINGLE-NUCLEOTIDE POLYMORPHISMS; DNA-SEQUENCING DATA; RNA-SEQ; GLYCINE-MAX; ARABIDOPSIS-THALIANA; GENOME SEQUENCE; MOLECULAR EVOLUTION; PROTEASE INHIBITORS; FUNCTIONAL MARKERS; GENETIC DIVERSITY AB Background: Variation in seed oil composition and content among soybean varieties is largely attributed to differences in transcript sequences and/or transcript accumulation of oil production related genes in seeds. Discovery and analysis of sequence and expression variations in these genes will accelerate soybean oil quality improvement. Results: In an effort to identify these variations, we sequenced the transcriptomes of soybean seeds from nine lines varying in oil composition and/or total oil content. Our results showed that 69,338 distinct transcripts from 32,885 annotated genes were expressed in seeds. A total of 8,037 transcript expression polymorphisms and 50,485 transcript sequence polymorphisms (48,792 SNPs and 1,693 small Indels) were identified among the lines. Effects of the transcript polymorphisms on their encoded protein sequences and functions were predicted. The studies also provided independent evidence that the lack of FAD2-1A gene activity and a non-synonymous SNP in the coding sequence of FAB2C caused elevated oleic acid and stearic acid levels in soybean lines M23 and FAM94-41, respectively. Conclusions: As a proof-of-concept, we developed an integrated RNA-seq and bioinformatics approach to identify and functionally annotate transcript polymorphisms, and demonstrated its high effectiveness for discovery of genetic and transcript variations that result in altered oil quality traits. The collection of transcript polymorphisms coupled with their predicted functional effects will be a valuable asset for further discovery of genes, gene variants, and functional markers to improve soybean oil quality. C1 [Goettel, Wolfgang; An, Yong-Qiang Charles] USDA ARS, Midwest Area, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Upchurch, Robert] USDA ARS, Raleigh, NC 27695 USA. [Wang, Ming-Li] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Chen, Pengyin] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. RP An, YQC (reprint author), USDA ARS, Midwest Area, Plant Genet Res Unit, Donald Danforth Plant Sci Ctr, 975 N Warson Rd, St Louis, MO 63132 USA. EM Yong-Qiang.An@ars.usda.gov FU United Soybean Board; USDA-ARS FX The authors would like to thank Rick Meyer for his wonderful technical support in computational data processing and analysis, Rick Meyer and anonymous reviewers for valuable comments on the manuscript, Dr. Joe Burton for sharing his genetic materials. This research is supported by funds from the United Soybean Board and USDA-ARS to Yong-qiang (Charles) An. NR 80 TC 7 Z9 9 U1 3 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 23 PY 2014 VL 15 AR 299 DI 10.1186/1471-2164-15-299 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AG4RL UT WOS:000335407800001 PM 24755115 ER PT J AU Doyle, JM Katzner, TE Bloom, PH Ji, YZ Wijayawardena, BK DeWoody, JA AF Doyle, Jacqueline M. Katzner, Todd E. Bloom, Peter H. Ji, Yanzhu Wijayawardena, Bhagya K. DeWoody, J. Andrew TI The Genome Sequence of a Widespread Apex Predator, the Golden Eagle (Aquila chrysaetos) SO PLOS ONE LA English DT Article ID MICROSATELLITE MARKERS; TRANSPOSABLE ELEMENTS; SPIZAETUS-NIPALENSIS; MITOCHONDRIAL-DNA; COLOR-VISION; HAWK-EAGLE; EVOLUTION; SIZE; ACCIPITRIDAE; POPULATION AB Biologists routinely use molecular markers to identify conservation units, to quantify genetic connectivity, to estimate population sizes, and to identify targets of selection. Many imperiled eagle populations require such efforts and would benefit from enhanced genomic resources. We sequenced, assembled, and annotated the first eagle genome using DNA from a male golden eagle (Aquila chrysaetos) captured in western North America. We constructed genomic libraries that were sequenced using Illumina technology and assembled the high-quality data to a depth of similar to 40x coverage. The genome assembly includes 2,552 scaffolds >10 Kb and 415 scaffolds >1.2 Mb. We annotated 16,571 genes that are involved in myriad biological processes, including such disparate traits as beak formation and color vision. We also identified repetitive regions spanning 92 Mb (similar to 6% of the assembly), including LINES, SINES, LTR-RTs and DNA transposons. The mitochondrial genome encompasses 17,332 bp and is similar to 91% identical to the Mountain Hawk-Eagle (Nisaetus nipalensis). Finally, the data reveal that several anonymous microsatellites commonly used for population studies are embedded within protein-coding genes and thus may not have evolved in a neutral fashion. Because the genome sequence includes similar to 800,000 novel polymorphisms, markers can now be chosen based on their proximity to functional genes involved in migration, carnivory, and other biological processes. C1 [Doyle, Jacqueline M.; Ji, Yanzhu; Wijayawardena, Bhagya K.; DeWoody, J. Andrew] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Wijayawardena, Bhagya K.; DeWoody, J. Andrew] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA. [Katzner, Todd E.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Katzner, Todd E.] USDA, Forest Serv, No Res Stn, Parsons, WV USA. [Bloom, Peter H.] Western Fdn Vertebrate Zool, Camarillo, CA USA. RP Doyle, JM (reprint author), Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. EM jmdoyle@purdue.edu RI DeWoody, James/C-4909-2008; OI DeWoody, James/0000-0002-7315-5631; Katzner, Todd/0000-0003-4503-8435 FU U.S. Bureau of Land Management [L12AC20102, L11PX02237, L12AC2010]; California Department of Fish and Wildlife [P1182024]; Provost's Office at Purdue University (University Faculty Scholar program) FX This work was supported by the U.S. Bureau of Land Management (award numbers L12AC20102, L11PX02237, and L12AC2010), the California Department of Fish and Wildlife (Agreement #P1182024), and the Provost's Office at Purdue University (University Faculty Scholar program). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 89 TC 13 Z9 13 U1 1 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 23 PY 2014 VL 9 IS 4 AR e95599 DI 10.1371/journal.pone.0095599 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG3EI UT WOS:000335298200068 PM 24759626 ER PT J AU Sankaran, RP Grusak, MA AF Sankaran, Renuka P. Grusak, Michael A. TI Whole shoot mineral partitioning and accumulation in pea (Pisum sativum) SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE PEA; mineral nutrition; remobilization; continuous uptake; seeds ID ARABIDOPSIS-THALIANA; DEVELOPING SEEDS; IRON-DEFICIENCY; FERRITIN GENE; SPRING WHEAT; LIFE-CYCLE; ZINC; TRANSPORT; METAL; REMOBILIZATION AB Several grain legumes are staple food crops that are important sources of minerals for humans; unfortunately, our knowledge is incomplete with respect to the mechanisms of translocation of these minerals to the vegetative tissues and loading into seeds. Understanding the mechanism and partitioning of minerals in pea could help in developing cultivars with high mineral density. A mineral partitioning study was conducted in pea to assess whole-plant growth and mineral content and the potential source-sink remobilization of different minerals, especially during seed development. Shoot and root mineral content increased for all the minerals, although tissue-specific partitioning differed between the minerals. Net remobilization was observed for P, S, Cu, and Fe from both the vegetative tissues and pod wall, but the amounts remobilized were much below the total accumulation in the seeds. Within the mature pod, more minerals were partitioned to the seed fraction (>75%) at maturity than to the pod wall for all the minerals except Ca, where only 21% was partitioned to the seed fraction. Although there was evidence for net remobilization of some minerals from different tissues into seeds, continued uptake and translocation of minerals to source tissues during seed fill is as important, if not more important, than remobilization of previously stored minerals. C1 [Sankaran, Renuka P.] CUNY Herbert H Lehman Coll, Dept Biol Sci, Bronx, NY 10468 USA. [Sankaran, Renuka P.] CUNY Grad Sch & Univ Ctr, New York, NY 10036 USA. [Grusak, Michael A.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Sankaran, RP (reprint author), CUNY Herbert H Lehman Coll, Dept Biol Sci, 250 Bedford Pk Blvd West, Bronx, NY 10468 USA. EM renuka.sankaran@lehman.cuny.edu FU USDA-ARS [58-6250-0-008] FX This work was funded in part by funds from USDA-ARS under Agreement Number 58-6250-0-008 to Michael A. Grusak. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organization simply endorsement by the US Government. NR 38 TC 6 Z9 6 U1 1 U2 19 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD APR 23 PY 2014 VL 5 AR 149 DI 10.3389/fpls.2014.00149 PG 8 WC Plant Sciences SC Plant Sciences GA AF6RQ UT WOS:000334842700001 PM 24795736 ER PT J AU Wang, LN Zhu, W Fang, LC Sun, XM Su, LY Liang, ZC Wang, N Londo, JP Li, SH Xin, HP AF Wang, Lina Zhu, Wei Fang, Linchuan Sun, Xiaoming Su, Lingye Liang, Zhenchang Wang, Nian Londo, Jason P. Li, Shaohua Xin, Haiping TI Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera SO BMC PLANT BIOLOGY LA English DT Article DE WRKY transcription factor family; Grapevine; Biotic and abiotic stress; Cold stress ID TRANSCRIPTION FACTOR FAMILY; DNA-BINDING PROTEINS; QUANTITATIVE PCR DATA; DROUGHT TOLERANCE; ABSCISIC-ACID; RALSTONIA-SOLANACEARUM; TRANSGENIC TOBACCO; SIGNALING PATHWAYS; FUNGAL PATHOGENS; SALINITY STRESS AB Background: WRKY transcription factors are one of the largest families of transcriptional regulators in plants. WRKY genes are not only found to play significant roles in biotic and abiotic stress response, but also regulate growth and development. Grapevine (Vitis vinifera) production is largely limited by stressful climate conditions such as cold stress and the role of WRKY genes in the survival of grapevine under these conditions remains unknown. Results: We identified a total of 59 VvWRKYs from the V. vinifera genome, belonging to four subgroups according to conserved WRKY domains and zinc-finger structure. The majority of VvWRKYs were expressed in more than one tissue among the 7 tissues examined which included young leaves, mature leaves, tendril, stem apex, root, young fruits and ripe fruits. Publicly available microarray data suggested that a subset of VvWRKYs was activated in response to diverse stresses. Quantitative real-time PCR (qRT-PCR) results demonstrated that the expression levels of 36 VvWRKYs are changed following cold exposure. Comparative analysis was performed on data from publicly available microarray experiments, previous global transcriptome analysis studies, and qRT-PCR. We identified 15 VvWRKYs in at least two of these databases which may relate to cold stress. Among them, the transcription of three genes can be induced by exogenous ABA application, suggesting that they can be involved in an ABA-dependent signaling pathway in response to cold stress. Conclusions: We identified 59 VvWRKYs from the V. vinifera genome and 15 of them showed cold stress-induced expression patterns. These genes represented candidate genes for future functional analysis of VvWRKYs involved in the low temperature-related signal pathways in grape. C1 [Wang, Lina; Zhu, Wei; Fang, Linchuan; Sun, Xiaoming; Wang, Nian; Li, Shaohua; Xin, Haiping] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China. [Su, Lingye; Liang, Zhenchang; Wang, Nian; Li, Shaohua; Xin, Haiping] Chinese Acad Sci, Inst Bot, Lab Plant Resources, Beijing Key Lab Grape Sci & Enol, Beijing, Peoples R China. [Wang, Lina; Zhu, Wei; Fang, Linchuan; Sun, Xiaoming; Su, Lingye; Liang, Zhenchang] Univ Chinese Acad Sci, Beijing, Peoples R China. [Londo, Jason P.] USDA ARS, Grape Genet Res Unit, Geneva, NY USA. RP Li, SH (reprint author), Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China. EM shhli@wbgcas.cn; xinhaiping215@hotmail.com OI /0000-0001-9185-4199 FU National Natural Science Foundation of China (NSFC) [31130047, 31000902] FX The authors thank Professor Yuepeng Han (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, The Chinese Academy of Sciences) for critical review of this manuscript. This work was supported by the National Natural Science Foundation of China (NSFC Accession No: 31130047, 31000902). NR 72 TC 25 Z9 31 U1 6 U2 112 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD APR 22 PY 2014 VL 14 AR 103 DI 10.1186/1471-2229-14-103 PG 14 WC Plant Sciences SC Plant Sciences GA AG3UF UT WOS:000335344600001 PM 24755338 ER PT J AU Ladinig, A Wilkinson, J Ashley, C Detmer, SE Lunney, JK Plastow, G Harding, JCS AF Ladinig, Andrea Wilkinson, Jamie Ashley, Carolyn Detmer, Susan E. Lunney, Joan K. Plastow, Graham Harding, John C. S. TI Variation in Fetal Outcome, Viral Load and ORF5 Sequence Mutations in a Large Scale Study of Phenotypic Responses to Late Gestation Exposure to Type 2 Porcine Reproductive and Respiratory Syndrome Virus SO PLOS ONE LA English DT Article ID TRANSPLACENTAL INFECTION; EXPERIMENTAL INOCULATION; MICROSCOPIC LESIONS; IMPLANTATION SITES; FETUSES; PIGS; PATHOGENESIS; GILTS; SWINE; PRRSV AB In spite of extensive research, the mechanisms of reproductive disease associated with Porcine Reproductive and Respiratory Syndrome virus (PRRSv) are still poorly understood. The objectives of this large scale study were to evaluate associations between viral load and fetal preservation, determine the impact of type 2 PRRSv on fetal weights, and investigate changes in ORF5 PRRSv genome in dams and fetuses during a 21-day period following challenge. At gestation day 85 (+/- 1), 114 gilts were experimentally infected with type 2 PRRSv, while 19 gilts served as reference controls. At necropsy, fetuses were categorized according to their preservation status and tissue samples were collected. PRRSv RNA concentrations were measured in gilt serum collected on days 0, 2, 6, and 21 post-infection, as well as in gilt and fetal tissues collected at termination. Fetal mortality was 41 +/- 22.8% in PRRS infected litters. Dead fetuses appeared to cluster in some litters but appeared solitary or random in others. Nine percent of surviving piglets were meconium-stained. PRRSv RNA concentration in fetal thymus, fetal serum and endometrium differed significantly across preservation category and was greatest in tissues of meconium-stained fetuses. This, together with the virtual absence of meconium staining in noninfected litters indicates it is an early pathological condition of reproductive PRRS. Viral load in fetal thymus and in fetal serum was positively associated with viral load in endometrium, suggesting the virus exploits dynamic linkages between individual maternal-fetal compartments. Point mutations in ORF5 sequences from gilts and fetuses were randomly located in 20 positions in ORF5, but neither nucleotide nor amino acid substitutions were associated with fetal preservation. PRRSv infection decreased the weights of viable fetuses by approximately 17%. The considerable variation in gilt and fetal outcomes provides tremendous opportunity for more detailed investigations of potential mechanisms and single nucleotide polymorphisms associated with fetal death. C1 [Ladinig, Andrea; Ashley, Carolyn; Harding, John C. S.] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. [Wilkinson, Jamie; Plastow, Graham] Univ Alberta, Fac Agr Life & Environm Sci, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. [Detmer, Susan E.] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK S7N 0W0, Canada. [Lunney, Joan K.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. RP Ladinig, A (reprint author), Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. EM andrea.ladinig@usask.ca OI Ladinig, Andrea/0000-0001-5037-7269 FU Genome Canada; Genome Alberta; Genome Prairie [2209-F] FX Funding for the project was generously provided by grants from Genome Canada, Genome Alberta and Genome Prairie (grant number 2209-F). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 12 Z9 12 U1 1 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 22 PY 2014 VL 9 IS 4 AR e96104 DI 10.1371/journal.pone.0096104 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG2IS UT WOS:000335240300137 PM 24756023 ER PT J AU Smirnova, NP Webb, BT McGill, JL Schaut, RG Bielefeldt-Ohmann, H Campen, H Sacco, RE Hansen, TR AF Smirnova, Natalia P. Webb, Brett T. McGill, Jodi L. Schaut, Robert G. Bielefeldt-Ohmann, Helle Van Campen, Hana Sacco, Randy E. Hansen, Thomas R. TI Induction of interferon-gamma and downstream pathways during establishment of fetal persistent infection with bovine viral diarrhea virus SO VIRUS RESEARCH LA English DT Article DE Bovine viral diarrhea virus; Fetus; Immune response; Interferon-gamma; Persistent infection; Chemokines ID DELTA T-CELLS; TRANSMEMBRANE CXC-CHEMOKINE; INNATE IMMUNE-RESPONSE; IFN-GAMMA; MUCOSAL DISEASE; NK CELLS; B-CELLS; CATTLE; EXPRESSION; BVDV AB Development of transplacental infection depends on the ability of the virus to cross the placenta and replicate within the fetus while counteracting maternal and fetal immune responses. Unfortunately, little is known about this complex process. Non-cytopathic (ncp) strains of bovine viral diarrhea virus (BVDV), a pestivirus in the Flaviviridae family, cause persistent infection in early gestational fetuses (<150 days; persistently infected, PI), but are cleared by immunocompetent animals and late gestational fetuses (>150 days; transiently infected, TI). Evasion of innate immune response and development of immunotolerance to ncp BVDV have been suggested as possible mechanisms for the establishment of the persistent infection. Previously we have observed a robust temporal induction of interferon (IFN) type I (innate immune response) and upregulation of IFN stimulated genes (ISGs) in BVDV TI fetuses. Modest chronic upregulation of ISGs in PI fetuses and calves reflects a stimulated innate immune response during persistent BVDV infection. We hypothesized that establishing persistent fetal BVDV infection is also accompanied by the induction of IFN-gamma (IFN-gamma). The aims of the present study were to determine IFN-gamma concentration in blood and amniotic fluid from control, TI and PI fetuses during BVDV infection and analyze induction of the IFN-gamma downstream pathways in fetal lymphoid tissues. Two experiments with in vivo BVDV infections were completed. In Experiment 1, pregnant heifers were infected with ncp BVDV type 2 on day 75 or 175 of gestation or kept naive to generate PI, TI and control fetuses, respectively. Fetuses were collected by Cesarean section on day 190. In Experiment 2, fetuses were collected on days 82, 89, 97, 192 and 245 following infection of pregnant heifers on day 75 of gestation. The results were consistent with the hypothesis that ncp BVDV infection induces IFN-gamma secretion during acute infection in both TI and PI fetuses and that lymphoid tissues such as spleen, liver and thymus, serve both as possible sources of IFN-gamma and target organs for its effects. Notably, induction of IFN-gamma coincides with a decrease in BVDV RNA concentrations in PI fetal blood and tissues. This is the first report indicating the possible presence of an C1 [Smirnova, Natalia P.; Webb, Brett T.; Hansen, Thomas R.] Colorado State Univ, Anim Reprod & Biotechnol Lab, Dept Biomed Sci, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. [Webb, Brett T.; Van Campen, Hana] Colorado State Univ, Dept Microbiol Immunol & Pathol, Coll Vet Med & Biomed Sci, Ft Collins, CO 80523 USA. [McGill, Jodi L.; Schaut, Robert G.; Sacco, Randy E.] USDA SEA, Ruminant Dis & Immunol Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Bielefeldt-Ohmann, Helle] Univ Queensland, Australian Infect Dis Res Ctr, St Lucia, Qld 4067, Australia. [Bielefeldt-Ohmann, Helle] Univ Queensland, Sch Vet Sci, Brisbane, Qld 4343, Australia. RP Hansen, TR (reprint author), Colorado State Univ, Anim Reprod & Biotechnol Lab, Dept Biomed Sci, Coll Vet Med & Biomed Sci, 1683 Campus Delivery, Ft Collins, CO 80523 USA. EM Natalia.Smirnova@colostate.edu; Brett.Webb@ndsu.edu; Jodi.McGill@ars.usda.gov; Robert-Schaut@uiowa.edu; h.bielefeldtohmann1@uq.edu.au; Hana.Van_Campen@Colostate.edu; Randy.Sacco@ars.usda.gov; Thomas.Hansen@colostate.edu OI Schaut, Robert G./0000-0003-2713-9013 FU Genomics Shared Resource of Colorado's NIH/NCI Cancer Center [P30CA046934]; USDA-NIFA-AFRI [2008-35204-04652] FX Authors would like to express their gratitude to Dr. Alfredo Q Antoniazzi and Dr. Cristina M. Weiner for performing Cesarean sections; Dr. Robert W. Norrdin, Dr. Luiz E. Henkes, Dr. Hyungchul Han, Dr. Jason E. Bruemmer, Aim-Elizabeth Hartwig, Dr. Juliano C. da Silveira, Dr. Ryan L Ashley (Colorado State University), Dr. Sue E. Morarie and Jessica E. Mediger (South Dakota State University) for the help with fetal collections; Jenna R. Tarbett, Krisaundra Hale, and Jared J. Romero (Colorado State University) for the technical assistance with processing blood samples; and Dr. Gao Bifeng and Dr. Tzu Phang (Core Facility at University of Colorado, Denver, supported in part by the Genomics Shared Resource of Colorado's NIH/NCI Cancer Center Support Grant P30CA046934) for completing microarray. Authors thank Richard C. Brandes, Zella Brink, and personnel of the Animal Reproduction and Biotechnology Laboratory for the help in management of the animals. The study was funded by USDA-NIFA-AFRI grant 2008-35204-04652. NR 55 TC 3 Z9 3 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD APR 21 PY 2014 VL 183 BP 95 EP 106 DI 10.1016/j.virusres.2014.02.002 PG 12 WC Virology SC Virology GA AE6UI UT WOS:000334133000015 PM 24530541 ER PT J AU Khir, R Atungulu, GG Pan, ZL Thompson, JF Zheng, X AF Khir, Ragab Atungulu, Griffiths G. Pan, Zhongli Thompson, James F. Zheng, Xia TI MOISTURE-DEPENDENT COLOR CHARACTERISTICS OF WALNUTS SO INTERNATIONAL JOURNAL OF FOOD PROPERTIES LA English DT Article DE Walnut; Shell; Moisture; Surface color; Sorting; Drying ID ROUGH RICE; QUALITY; STORAGE AB Characterizing the shell color of walnuts based on their moisture content at harvest can provide valuable information for performing walnut sorting before drying. The objective of this study was to investigate the relationship between the color characteristics of the shell of walnuts and their moisture content. Measurements were carried out for three walnut varieties, Tulare, Howard, and Chandler. The walnuts were sorted into two categories, namely with and without hulls at harvest. The Commission International de l'Esclairage's L*, a*, and b* color indices were measured to quantify the shell color after hull removal, and total color difference, hue angle (h*), and chroma (C*) values were calculated. The results indicated that the moisture content of walnuts with hulls at harvest was much higher than that of walnuts without hulls. The L* and E values are highly correlated with moisture content and had different trends for walnuts with and without hulls. There was no clear relationship between other color characteristics and moisture content, although the a* values of walnuts with hulls were higher than those of walnuts without hulls. The results obtained revealed the potential of using the relationship between color indices of L* or E and walnut shell moisture content to sort walnuts before drying, which is essential to avoid over-drying, increase drying capacity, reduce energy use, and obtain high quality walnut products. C1 [Khir, Ragab; Atungulu, Griffiths G.; Pan, Zhongli; Thompson, James F.] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Khir, Ragab] Suez Canal Univ, Fac Agr, Dept Agr Engn, Ismailia, Egypt. [Pan, Zhongli] USDA ARS WRRC, Proc Foods Res Unit, Albany, CA USA. [Zheng, Xia] Shihezhi Univ, Coll Mech & Elect Engn, Shihezi, Xinjiang, Peoples R China. RP Pan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, 1 Shields Ave, Davis, CA 95616 USA. EM Zhongli.Pan@ars.usda.gov FU California Walnut Board FX The authors wish to thank the California Walnut Board for its partial financial support, and Cilker Orchards for their generosity in supplying walnut samples used throughout this research. The research was conducted at the Western Regional Research Center of USDA-ARS and Department of Biological and Agricultural Engineering, University of California, Davis, California, USA. NR 33 TC 4 Z9 4 U1 4 U2 34 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 325 CHESTNUT ST, SUITE 800, PHILADELPHIA, PA 19106 USA SN 1094-2912 EI 1532-2386 J9 INT J FOOD PROP JI Int. J. Food Prop. PD APR 21 PY 2014 VL 17 IS 4 BP 877 EP 890 DI 10.1080/10942912.2012.675610 PG 14 WC Food Science & Technology SC Food Science & Technology GA 254SP UT WOS:000327187600015 ER PT J AU Hu, E Bosworth, B Baxter, J Tiersch, TR AF Hu, E. Bosworth, Brian Baxter, Jeff Tiersch, Terrence R. TI On-site evaluation of commercial-scale hybrid catfish production using cryopreserved blue catfish sperm SO AQUACULTURE LA English DT Article DE Blue catfish; Commercial application; Sperm cryopreservation; Pooled vs. individual samples ID PANGASIUS-HYPOPHTHALMUS SAUVAGE; ICTALURUS-PUNCTATUS EGGS; CHANNEL CATFISH; CLARIAS-GARIEPINUS; FERTILIZATION EFFICIENCY; CRASSOSTREA-GIGAS; STRIPED CATFISH; PACIFIC OYSTERS; FREEZING RATES; SPERMATOZOA AB Cryopreservation is an effective tool for conservation of genetic resources and is becoming increasingly used worldwide with aquatic species. Broadening the application of this technology to a commercial scale through high-throughput approaches has become essential for use with aquatic species. This study addressed high-throughput sperm cryopreservation of blue catfish at an industrial level. Our objectives were to: 1) optimize the sperm volume used for thawed sperm; 2) evaluate commercial application of high-throughput cryopreserved sperm with standard hatchery techniques, and 3) initiate evaluation of the fertility relationship between individuals and pooled samples. The results showed that a doubling of the previously established volume did not produce significant improvement in fertilization. The working volume of thawed sperm(2 ml at a concentration of 1 x 10(9)/ml for batches of 100-150ml channel catfish eggs) was practical. There was no significant difference in fry production after artificial fertilization of 2 million eggs with cryopreserved or fresh sperm. Pooled sperm samples and the individual samples used to form the pools produced similar fertilization rates. Blue catfish sperm is valued as a genetic material for hybrid catfish production, and cryopreservation makes genetic material management possible. This study initiates industrialization of this technology for use with aquatic organisms, and because the technology can be generalized, expands the opportunities for application to other species. High-throughput cryopreservation of blue catfish sperm provides new capabilities and can maintain sperm quality sufficiently to support commercial hybrid production. (C) 2014 Elsevier B.V. All rights reserved. C1 [Hu, E.; Tiersch, Terrence R.] Louisiana State Univ, Ctr Agr, Louisiana Agr Expt Stn, Aquaculture Res Stn, Baton Rouge, LA 70803 USA. [Bosworth, Brian] USDA, Catfish Genet Res Unit, Stoneville, MS 38756 USA. [Baxter, Jeff] Baxter Land Co, Dermott, AR 71638 USA. RP Tiersch, TR (reprint author), 2410 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM ttiersch@agcenter.lsu.edu FU Louisiana Sea Grant College Program; USDA; USDA-SBIR; National Center for Research Resources of the National Institutes of Health FX We thank C. Staudermann, D. Kuenz, J. Atilano, J. Christensen, J. Tanca, N. Novelo, R. Alfonso and R. Uribe for technical assistance during the spawning season, data collection, and for suggestions. We thank C. Green and H. Yang for the advice during experiments, and N. Novelo and C. Fisher for the friendly review. This work was supported in part by funding from the Louisiana Sea Grant College Program, USDA special grants, USDA-SBIR, and the National Center for Research Resources of the National Institutes of Health. This manuscript has been approved for publication by the Director of the Louisiana Agricultural Experiment Station as number (2013-244-8174). NR 49 TC 5 Z9 5 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD APR 20 PY 2014 VL 426 BP 88 EP 95 DI 10.1016/j.aquaculture.2014.01.024 PG 8 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AE8WP UT WOS:000334284000013 ER PT J AU Li, H Zhang, AJ Chen, LZ Zhang, GA Wang, MQ AF Li, Hui Zhang, Aijun Chen, Li-Zhen Zhang, Guoan Wang, Man-Qun TI Construction and analysis of cDNA libraries from the antennae of Batocera horsfieldi and expression pattern of putative odorant binding proteins SO JOURNAL OF INSECT SCIENCE LA English DT Article DE Antennal cDNA library; Minus-C OBPs; RT-PCR; qPCR ID OLFACTORY RECEPTOR NEURONS; PHEROMONE-BINDING; ANOPHELES-GAMBIAE; DROSOPHILA-MELANOGASTER; CHEMOSENSORY PROTEINS; MOLECULAR-CLONING; GENE-EXPRESSION; MOTH ANTENNAE; IDENTIFICATION; MOSQUITO AB A high-quality cDNA library was constructed from female and male antenna of the longhorned beetle, Batocera horsfieldi (Hope) (Coleoptera: Cerambycidae), a serious pest of Populus (Salicales: Salicaceae). The titer was approximately 2.37 x 10(6) pfu/mL, and this complies with the test requirement. From the libraries, 692 clones were selected randomly, sequenced, and further analyzed, and the recombinational efficiency reached 93.85%. By alignment and cluster analysis, we identified four odorant binding proteins, two pheromone-binding proteins (have the characteristic six conserved cysteine residues), four Minus-C odorant binding proteins (lost two conserved cysteines), and three chemosensory proteins. In this study, we describe the identification and characterization of four new cDNAs that encode Minus-C odorant binding proteins (Minus-C OBPs) from B. horsfieldi antennal cDNA libraries. Our investigation focused on the expression pattern of the Minus-C OBP genes in various tissues in both sexes at different developmental stages, using reverse transcription PCR (RT-PCR) and real-time PCR (qPCR) strategies. Minus-C OBP1, 2, and 3 were expressed in all tested tissues, with the exception of the head (without antenna, labial palps, and maxillary palps). Minus-C OBP4 was expressed in the antenna, legs, and abdomen, but not in the labial palps, maxillary palps, or head. The qPCR results revealed Minus-C OBPs were expressed in the antenna throughout the adult life, and that the transcript levels of these genes depended on the sex, age, and mating status of adults. C1 [Li, Hui; Chen, Li-Zhen; Zhang, Guoan; Wang, Man-Qun] Huazhong Agr Univ, Hubei Insect Resources Utilizat & Sustainable Pes, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China. [Li, Hui] Henan Univ Technol, Zhengzhou 450001, Peoples R China. [Zhang, Aijun] USDA ARS, Invas Insect Biocontrol & Behav Lab, Inst Plant Sci, Beltsville, MD 20705 USA. RP Wang, MQ (reprint author), Huazhong Agr Univ, Hubei Insect Resources Utilizat & Sustainable Pes, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China. EM mqwang@mail.hzau.edu.cn FU National Natural Science Foundation of China [30972374]; Special Fund for Agro-Scientific Research in the Public Interest of China [201203036]; doctoral Foundation of Henan University of Technology [2014BS010] FX This work was funded by the National Natural Science Foundation of China (No. 30972374), Special Fund for Agro-Scientific Research in the Public Interest of China (201203036), and the doctoral Foundation of Henan University of Technology (2014BS010). NR 43 TC 5 Z9 5 U1 1 U2 18 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1536-2442 EI 2250-2645 J9 J INSECT SCI JI J Insect Sci. PD APR 19 PY 2014 VL 14 AR 57 PG 15 WC Entomology SC Entomology GA AF6MC UT WOS:000334828200006 PM 25373204 ER PT J AU Gilbert, MK Kim, HJ Tang, YH Naoumkina, M Fang, DD AF Gilbert, Matthew K. Kim, Hee Jin Tang, Yuhong Naoumkina, Marina Fang, David D. TI Comparative Transcriptome Analysis of Short Fiber Mutants Ligon-Lintless 1 And 2 Reveals Common Mechanisms Pertinent to Fiber Elongation in Cotton (Gossypium hirsutum L.) SO PLOS ONE LA English DT Article ID PECTIN METHYL ESTERASE; GENE-EXPRESSION; DOWN-REGULATION; PHANEROCHAETE-CHRYSOSPORIUM; GLYOXAL OXIDASE; CELL EXPANSION; LI-1 MUTANT; IN-VITRO; GROWTH; WALL AB Understanding the molecular processes affecting cotton (Gossypium hirsutum) fiber development is important for developing tools aimed at improving fiber quality. Short fiber cotton mutants Ligon-lintless 1 (Li-1) and Ligon-lintless 2 (Li-2) are naturally occurring, monogenic mutations residing on different chromosomes. Both mutations cause early cessation in fiber elongation. These two mutants serve as excellent model systems to elucidate molecular mechanisms relevant to fiber length development. Previous studies of these mutants using transcriptome analysis by our laboratory and others had been limited by the fact that very large numbers of genes showed altered expression patterns in the mutants, making a targeted analysis difficult or impossible. In this research, a comparative microarray analysis was conducted using these two short fiber mutants and their near isogenic wild type (WT) grown under both field and greenhouse environments in order to identify key genes or metabolic pathways common to fiber elongation. Analyses of three transcriptome profiles obtained from different growth conditions and mutant types showed that most differentially expressed genes (DEGs) were affected by growth conditions. Under field conditions, short fiber mutants commanded higher expression of genes related to energy production, manifested by the increasing of mitochondrial electron transport activity or responding to reactive oxygen species when compared to the WT. Eighty-eight DEGs were identified to have altered expression patterns common to both short fiber mutants regardless of growth conditions. Enrichment, pathway and expression analyses suggested that these 88 genes were likely involved in fiber elongation without being affected by growth conditions. C1 [Gilbert, Matthew K.; Kim, Hee Jin; Naoumkina, Marina; Fang, David D.] USDA ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. [Tang, Yuhong] Samuel Roberts Noble Fdn Inc, Genom Core Facil, Ardmore, OK USA. RP Fang, DD (reprint author), USDA ARS, Cotton Fiber Biosci Res Unit, So Reg Res Ctr, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459 FU USDA-ARS CRIS [6435-21000-017D] FX The research was funded by the USDA-ARS CRIS project 6435-21000-017D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 69 TC 8 Z9 8 U1 1 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 18 PY 2014 VL 9 IS 4 AR e95554 DI 10.1371/journal.pone.0095554 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG2DP UT WOS:000335226500123 PM 24748059 ER PT J AU Schilling, MP Wolf, PG Duffy, AM Rai, HS Rowe, CA Richardson, BA Mock, KE AF Schilling, Martin P. Wolf, Paul G. Duffy, Aaron M. Rai, Hardeep S. Rowe, Carol A. Richardson, Bryce A. Mock, Karen E. TI Genotyping-by-Sequencing for Populus Population Genomics: An Assessment of Genome Sampling Patterns and Filtering Approaches SO PLOS ONE LA English DT Article ID GENETIC-VARIATION; BALSAM POPLAR; SNP DISCOVERY; DIVERSITY; TREMULOIDES; CONSERVATION; POLYMORPHISM; SELECTION; PACKAGE; MARKERS AB Continuing advances in nucleotide sequencing technology are inspiring a suite of genomic approaches in studies of natural populations. Researchers are faced with data management and analytical scales that are increasing by orders of magnitude. With such dramatic advances comes a need to understand biases and error rates, which can be propagated and magnified in large-scale data acquisition and processing. Here we assess genomic sampling biases and the effects of various population-level data filtering strategies in a genotyping-by-sequencing (GBS) protocol. We focus on data from two species of Populus, because this genus has a relatively small genome and is emerging as a target for population genomic studies. We estimate the proportions and patterns of genomic sampling by examining the Populus trichocarpa genome (Nisqually-1), and demonstrate a pronounced bias towards coding regions when using the methylation-sensitive ApeKI restriction enzyme in this species. Using population-level data from a closely related species (P. tremuloides), we also investigate various approaches for filtering GBS data to retain high-depth, informative SNPs that can be used for population genetic analyses. We find a data filter that includes the designation of ambiguous alleles resulted in metrics of population structure and Hardy-Weinberg equilibrium that were most consistent with previous studies of the same populations based on other genetic markers. Analyses of the filtered data (27,910 SNPs) also resulted in patterns of heterozygosity and population structure similar to a previous study using microsatellites. Our application demonstrates that technically and analytically simple approaches can readily be developed for population genomics of natural populations. C1 [Schilling, Martin P.; Wolf, Paul G.; Duffy, Aaron M.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Rai, Hardeep S.; Rowe, Carol A.; Mock, Karen E.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Richardson, Bryce A.] USDA Forest Serv, Rocky Mt Res Stn, Provo, UT USA. [Schilling, Martin P.; Wolf, Paul G.; Mock, Karen E.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. RP Mock, KE (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM karen.mock@usu.edu RI Mock , Karen/C-1418-2011; Wolf, Paul/F-7664-2010 OI Wolf, Paul/0000-0002-4317-6976 FU USDA Forest Service Western Forest Transcriptome Survey and National Fire Plan [2012-NFP-GSD-1] FX This research was funded by the USDA Forest Service Western Forest Transcriptome Survey and National Fire Plan (2012-NFP-GSD-1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 6 Z9 6 U1 7 U2 69 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 18 PY 2014 VL 9 IS 4 AR e95292 DI 10.1371/journal.pone.0095292 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG2DP UT WOS:000335226500078 PM 24748384 ER PT J AU Xia, Y Lei, L Brabham, C Stork, J Strickland, J Ladak, A Gu, Y Wallace, I DeBolt, S AF Xia, Ye Lei, Lei Brabham, Chad Stork, Jozsef Strickland, James Ladak, Adam Gu, Ying Wallace, Ian DeBolt, Seth TI Acetobixan, an Inhibitor of Cellulose Synthesis Identified by Microbial Bioprospecting SO PLOS ONE LA English DT Article ID ARABIDOPSIS-THALIANA; SYNTHASE COMPLEXES; PLASMA-MEMBRANE; BACTERIAL IDENTIFICATION; FUNCTIONAL ASSOCIATION; HERBICIDE ISOXABEN; GENETIC-EVIDENCE; PLANT-GROWTH; BIOSYNTHESIS; MICROTUBULES AB In plants, cellulose biosynthesis is an essential process for anisotropic growth and therefore is an ideal target for inhibition. Based on the documented utility of small-molecule inhibitors to dissect complex cellular processes we identified a cellulose biosynthesis inhibitor (CBI), named acetobixan, by bio-prospecting among compounds secreted by endophytic microorganisms. Acetobixan was identified using a drug-gene interaction screen to sift through hundreds of endophytic microbial secretions for one that caused synergistic reduction in root expansion of the leaky AtcesA6(prc1-1) mutant. We then mined this microbial secretion for compounds that were differentially abundant compared with Bacilli that failed to mimic CBI action to isolate a lead pharmacophore. Analogs of this lead compound were screened for CBI activity, and the most potent analog was named acetobixan. In living Arabidopsis cells visualized by confocal microscopy, acetobixan treatment caused CESA particles localized at the plasma membrane (PM) to rapidly re-localize to cytoplasmic vesicles. Acetobixan inhibited C-14-Glc uptake into crystalline cellulose. Moreover, cortical microtubule dynamics were not disrupted by acetobixan, suggesting specific activity towards cellulose synthesis. Previous CBI resistant mutants such as ixr1-2, ixr2-1 or aegeus were not cross resistant to acetobixan indicating that acetobixan targets a different aspect of cellulose biosynthesis. C1 [Xia, Ye; Brabham, Chad; Stork, Jozsef; DeBolt, Seth] Univ Kentucky, Dept Hort, Lexington, KY 40546 USA. [Lei, Lei; Gu, Ying] Penn State Univ, Dept Biochem & Mol Biol, State Coll, PA USA. [Strickland, James] ARS, USDA, Forage Anim Prod Res Unit, Lexington, KY USA. [Ladak, Adam] Waters Waters Corp, Milford, MA USA. [Wallace, Ian] Univ Nevada, Dept Biochem & Mol Biol, Reno, NV 89557 USA. RP DeBolt, S (reprint author), Univ Kentucky, Dept Hort, Lexington, KY 40546 USA. EM sdebo2@uky.edu OI Lei, Lei/0000-0003-4803-4763 FU National Science Foundation [NSF-IOS-0922947]; Department of Energy [DOE-FOA10-0000368]; NSF-MCB [1121375] FX This study was supported by a grant from the National Science Foundation grants NSF-IOS-0922947 and Department of Energy DOE-FOA10-0000368 (SD) and NSF-MCB 1121375 (YG). The authors thank William Underwood critical reading of the manuscript and Chris Somerville for the financial support of IW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 6 Z9 6 U1 0 U2 20 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 18 PY 2014 VL 9 IS 4 AR e95245 DI 10.1371/journal.pone.0095245 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG2DP UT WOS:000335226500070 PM 24748166 ER PT J AU Crim, SM Iwamoto, M Huang, JY Griffin, PM Gilliss, D Cronquist, AB Cartter, M Tobin-D'Angelo, M Blythe, D Smith, K Lathrop, S Zansky, S Cieslak, PR Dunn, J Holt, KG Lance, S Tauxe, R Henao, OL AF Crim, Stacy M. Iwamoto, Martha Huang, Jennifer Y. Griffin, Patricia M. Gilliss, Debra Cronquist, Alicia B. Cartter, Matthew Tobin-D'Angelo, Melissa Blythe, David Smith, Kirk Lathrop, Sarah Zansky, Shelley Cieslak, Paul R. Dunn, John Holt, Kristin G. Lance, Susan Tauxe, Robert Henao, Olga L. TI Incidence and Trends of Infection with Pathogens Transmitted Commonly Through Food - Foodborne Diseases Active Surveillance Network, 10 US Sites, 2006-2013 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID UNITED-STATES C1 [Crim, Stacy M.; Iwamoto, Martha; Huang, Jennifer Y.; Griffin, Patricia M.; Tauxe, Robert; Henao, Olga L.] CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. [Gilliss, Debra] California Dept Publ Hlth, Sacramento, CA USA. [Cronquist, Alicia B.] Colorado Dept Publ Hlth & Environm, Arvada, CO USA. [Tobin-D'Angelo, Melissa] Georgia Dept Publ Hlth, Atlanta, GA USA. [Blythe, David] Maryland Dept Hlth & Mental Hyg, Baltimore, MD 21201 USA. [Smith, Kirk] Minnesota Dept Hlth, Minneapolis, MN 55414 USA. [Lathrop, Sarah] Univ New Mexico, Albuquerque, NM 87131 USA. [Zansky, Shelley] New York State Dept Hlth, Albany, NY 12237 USA. [Cieslak, Paul R.] Oregon Hlth Author, Salem, OR USA. [Dunn, John] Tennessee Dept Hlth, Memphis, TN USA. [Holt, Kristin G.] US Food Safety & Inspect Serv, USDA, Washington, DC 20250 USA. [Lance, Susan] US FDA, Ctr Food Safety & Appl Nutr, Rockville, MD 20857 USA. RP Henao, OL (reprint author), CDC, Div Foodborne Waterborne & Environm Dis, Natl Ctr Emerging & Zoonot Infect Dis, Atlanta, GA 30333 USA. EM ohenao@cdc.gov NR 10 TC 78 Z9 79 U1 3 U2 26 PU CENTER DISEASE CONTROL & PREVENTION PI ATLANTA PA MAILSTOP E-90, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD APR 18 PY 2014 VL 63 IS 15 BP 328 EP 332 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AF1VY UT WOS:000334503200003 PM 24739341 ER PT J AU Venu, RC Ma, JB Jia, YL Liu, GJ Jia, MH Nobuta, K Sreerekha, MV Moldenhauer, K McClung, AM Meyers, BC Wang, GL AF Venu, R. C. Ma, Jianbing Jia, Yulin Liu, Guangjie Jia, Melissa H. Nobuta, Kan Sreerekha, M. V. Moldenhauer, Karen McClung, Anna M. Meyers, Blake C. Wang, Guo-Liang TI Identification of Candidate Genes Associated with Positive and Negative Heterosis in Rice SO PLOS ONE LA English DT Article ID QUANTITATIVE TRAIT LOCUS; ORYZA-SATIVA L.; CONTROLLING HEADING DATE; TRANSCRIPTOME ANALYSIS; GRAIN WIDTH; NATURAL VARIATION; GREEN-REVOLUTION; FLOWERING TIME; DRAFT SEQUENCE; MAJOR QTL AB To identify the genes responsible for yield related traits, and heterosis, massively parallel signature sequencing (MPSS) libraries were constructed from leaves, roots and meristem tissues from the two parents, 'Nipponbare' and '93-11', and their F-1 hybrid. From the MPSS libraries, 1-3 million signatures were obtained. Using cluster analysis, commonly and specifically expressed genes in the parents and their F-1 hybrid were identified. To understand heterosis in the F-1 hybrid, the differentially expressed genes in the F-1 hybrid were mapped to yield related quantitative trait loci (QTL) regions using a linkage map constructed from 131 polymorphic simple sequence repeat markers with 266 recombinant inbred lines derived from a cross between Nipponbare and 93-11. QTLs were identified for yield related traits including days to heading, plant height, plant type, number of tillers, main panicle length, number of primary branches per main panicle, number of kernels per main panicle, total kernel weight per main panicle, 1000 grain weight and total grain yield per plant. Seventy one QTLs for these traits were mapped, of which 3 QTLs were novel. Many highly expressed chromatin-related genes in the F-1 hybrid encoding histone demethylases, histone deacetylases, argonaute-like proteins and polycomb proteins were located in these yield QTL regions. A total of 336 highly expressed transcription factor (TF) genes belonging to 50 TF families were identified in the yield QTL intervals. These findings provide the starting genomic materials to elucidate the molecular basis of yield related traits and heterosis in rice. C1 [Venu, R. C.; Ma, Jianbing; Jia, Yulin; Liu, Guangjie; Jia, Melissa H.; McClung, Anna M.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Venu, R. C.; Ma, Jianbing; Liu, Guangjie; Moldenhauer, Karen] Univ Arkansas, Div Agr, Rice Res & Extens Ctr, Stuttgart, AR USA. [Venu, R. C.; Sreerekha, M. V.; Wang, Guo-Liang] Ohio State Univ, Dept Plant Pathol, Columbus, OH 43210 USA. [Nobuta, Kan; Meyers, Blake C.] Univ Delaware, Delaware Biotechnol Inst, Newark, DC USA. RP Jia, YL (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM yulin.jia@ars.usda.gov RI Meyers, Blake/B-6535-2012 OI Meyers, Blake/0000-0003-3436-6097 FU Plant Genome Research Program of the National Science Foundation [0701745, 0321437] FX This project was supported in part by the Plant Genome Research Program of the National Science Foundation [Award numbers: 0701745 and 0321437]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 86 TC 3 Z9 4 U1 3 U2 41 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 17 PY 2014 VL 9 IS 4 AR e95178 DI 10.1371/journal.pone.0095178 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG3IK UT WOS:000335309100081 PM 24743656 ER PT J AU Chambers, AH Pillet, J Plotto, A Bai, JH Whitaker, VM Folta, KM AF Chambers, Alan H. Pillet, Jeremy Plotto, Anne Bai, Jinhe Whitaker, Vance M. Folta, Kevin M. TI Identification of a strawberry flavor gene candidate using an integrated genetic-genomic-analytical chemistry approach SO BMC GENOMICS LA English DT Article DE RNA-seq; gamma-decalactone; Fragaria; Flavor; Molecular marker; Strawberry ID FRAGARIA X ANANASSA; OCTOPLOID STRAWBERRY; AROMA COMPOUNDS; HARVEST DATE; FRUIT; GENERATION; WILD; BIOSYNTHESIS; BIOCATALYSIS; INHERITANCE AB Background: There is interest in improving the flavor of commercial strawberry (Fragaria x ananassa) varieties. Fruit flavor is shaped by combinations of sugars, acids and volatile compounds. Many efforts seek to use genomics-based strategies to identify genes controlling flavor, and then designing durable molecular markers to follow these genes in breeding populations. In this report, fruit from two cultivars, varying for presence-absence of volatile compounds, along with segregating progeny, were analyzed using GC/MS and RNAseq. Expression data were bulked in silico according to presence/absence of a given volatile compound, in this case gamma-decalactone, a compound conferring a peach flavor note to fruits. Results: Computationally sorting reads in segregating progeny based on gamma-decalactone presence eliminated transcripts not directly relevant to the volatile, revealing transcripts possibly imparting quantitative contributions. One candidate encodes an omega-6 fatty acid desaturase, an enzyme known to participate in lactone production in fungi, noted here as FaFAD1. This candidate was induced by ripening, was detected in certain harvests, and correlated with gamma-decalactone presence. The FaFAD1 gene is present in every genotype where gamma-decalactone has been detected, and it was invariably missing in non-producers. A functional, PCR-based molecular marker was developed that cosegregates with the phenotype in F-1 and BC1 populations, as well as in many other cultivars and wild Fragaria accessions. Conclusions: Genetic, genomic and analytical chemistry techniques were combined to identify FaFAD1, a gene likely controlling a key flavor volatile in strawberry. The same data may now be re-sorted based on presence/absence of any other volatile to identify other flavor-affecting candidates, leading to rapid generation of gene-specific markers. C1 [Chambers, Alan H.; Pillet, Jeremy; Folta, Kevin M.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Plotto, Anne; Bai, Jinhe] ARS, Hort Res Lab, USDA, Ft Pierce, FL USA. [Whitaker, Vance M.] Univ Florida, Gulf Coast Res & Educ Ctr, Gainesville, FL USA. [Folta, Kevin M.] Univ Florida, Plant Mol & Cellular Biol Program, Gainesville, FL USA. RP Folta, KM (reprint author), Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. EM kfolta@ufl.edu FU University of Florida Plant Molecular Breeding Initiative; Florida Strawberry Research and Education Foundation FX The authors thank Dr. Jeffrey Brecht (University of Florida) for the loan of a Chemstation software license, and Paul V. Stodghill (Robert W. Holley Center for Agriculture and Health, Agriculture Research Service, USDA, Ithaca, NY) for their assistance with the project. The authors also thank Shane Alan Evans and Christy Evans for their assistance in processing fruit samples. This work was performed with funding from the University of Florida Plant Molecular Breeding Initiative, with support from the Florida Strawberry Research and Education Foundation. NR 44 TC 12 Z9 13 U1 5 U2 73 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 17 PY 2014 VL 15 AR 217 DI 10.1186/1471-2164-15-217 PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AF8EM UT WOS:000334948300001 PM 24742080 ER PT J AU Busch, JD Stone, NE Nottingham, R Araya-Anchetta, A Lewis, J Hochhalter, C Giles, JR Gruendike, J Freeman, J Buckmeier, G Bodine, D Duhaime, R Miller, RJ Davey, RB Olafson, PU Scoles, GA Wagner, DM AF Busch, Joseph D. Stone, Nathan E. Nottingham, Roxanne Araya-Anchetta, Ana Lewis, Jillian Hochhalter, Christian Giles, John R. Gruendike, Jeffrey Freeman, Jeanne Buckmeier, Greta Bodine, Deanna Duhaime, Roberta Miller, Robert J. Davey, Ronald B. Olafson, Pia U. Scoles, Glen A. Wagner, David M. TI Widespread movement of invasive cattle fever ticks (Rhipicephalus microplus) in southern Texas leads to shared local infestations on cattle and deer SO PARASITES & VECTORS LA English DT Article DE Bovine babesiosis; Cattle fever ticks; Eradication; Population genetics; Rhipicephalus (Boophilus) microplus ID BOOPHILUS-MICROPLUS; UNITED-STATES; POPULATION-STRUCTURE; 1ST REPORT; PYRETHROID RESISTANCE; MICROSATELLITE LOCI; ACARI IXODIDAE; NEW-CALEDONIA; F-STATISTICS; MEXICO AB Background: Rhipicephalus (Boophilus) microplus is a highly- invasive tick that transmits the cattle parasites (Babesia bovis and B. bigemina) that cause cattle fever. R. microplus and Babesia are endemic in Mexico and ticks persist in the United States inside a narrow tick eradication quarantine area (TEQA) along the Rio Grande. This containment area is threatened by unregulated movements of illegal cattle and wildlife like white-tailed deer (WTD; Odocoileus virginianus). Methods: Using 11 microsatellite loci we genotyped 1,247 R. microplus from 63 Texas collections, including outbreak infestations from outside the TEQA. We used population genetic analyses to test hypotheses about ecological persistence, tick movement, and impacts of the eradication program in southern Texas. We tested acaricide resistance with larval packet tests (LPTs) on 47 collections. Results: LPTs revealed acaricide resistance in 15/47 collections (32%); 11 were outside the TEQA and three were resistant to multiple acaricides. Some collections highly resistant to permethrin were found on cattle and WTD. Analysis of genetic differentiation over time at seven properties revealed local gene pools with very low levels of differentiation (F-ST 0.00-0.05), indicating persistence over timespans of up to 29 months. However, in one neighborhood differentiation varied greatly over a 12-month period (F-ST 0.03-0.13), suggesting recurring immigration from distinct sources as another persistence mechanism. Ticks collected from cattle and WTD at the same location are not differentiated (F-ST = 0), implicating ticks from WTD as a source of ticks on cattle (and vice versa) and emphasizing the importance of WTD to tick control strategies. We identified four major genetic groups (K = 4) using Bayesian population assignment, suggesting multiple introductions to Texas. Conclusions: Two dispersal mechanisms give rise to new tick infestations: 1) frequent short-distance dispersal from the TEQA; and 2) rare long-distance, human-mediated dispersal from populations outside our study area, probably Mexico. The threat of cattle fever tick transport into Texas is increased by acaricide resistance and the ability of R. microplus to utilize WTD as an alternate host. Population genetic analyses may provide a powerful tool for tracking invasions in other parts of the world where these ticks are established. C1 [Busch, Joseph D.; Stone, Nathan E.; Nottingham, Roxanne; Araya-Anchetta, Ana; Lewis, Jillian; Hochhalter, Christian; Giles, John R.; Gruendike, Jeffrey; Wagner, David M.] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA. [Freeman, Jeanne; Buckmeier, Greta; Bodine, Deanna; Olafson, Pia U.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Duhaime, Roberta] USDA APHIS, Vet Serv, Austin, TX 78701 USA. [Miller, Robert J.; Davey, Ronald B.] USDA ARS, SPA, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. [Scoles, Glen A.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. RP Wagner, DM (reprint author), No Arizona Univ, Ctr Microbial Genet & Genom, 1298 S Knoles Dr,POB 4073, Flagstaff, AZ 86011 USA. EM Dave.Wagner@nau.edu FU USDA-NIFA [2010-65104-20386] FX We thank USDA-APHIS mounted patrol inspectors for collecting field samples used in this study. We are grateful to Mr. Ed Bowers, Director of Field Operations (CFTEP) for providing field records of infested properties and valuable insight into the eradication program. This work was supported by USDA-NIFA Grant 2010-65104-20386. The USDA is an equal opportunity provider and employer. Use of trade, product, or firm names does not imply endorsement by the US Government. NR 56 TC 13 Z9 13 U1 4 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD APR 17 PY 2014 VL 7 AR 188 DI 10.1186/1756-3305-7-188 PG 16 WC Parasitology SC Parasitology GA AF9ZF UT WOS:000335072500001 PM 24742041 ER PT J AU Giles, JR Peterson, AT Busch, JD Olafson, PU Scoles, GA Davey, RB Pound, JM Kammlah, DM Lohmeyer, KH Wagner, DM AF Giles, John R. Peterson, A. Townsend Busch, Joseph D. Olafson, Pia U. Scoles, Glen A. Davey, Ronald B. Pound, J. Mathews Kammlah, Diane M. Lohmeyer, Kimberly H. Wagner, David M. TI Invasive potential of cattle fever ticks in the southern United States SO PARASITES & VECTORS LA English DT Article ID BOOPHILUS-MICROPLUS ACARI; IXODIDAE POPULATION-DYNAMICS; DEER ODOCOILEUS-VIRGINIANUS; AMBLYOMMA-AMERICANUM ACARI; ARTHROPOD-BORNE PATHOGENS; SPECIES DISTRIBUTION; CLIMATE-CHANGE; DISTRIBUTION MODELS; GLOBAL CHANGE; ECOLOGICAL PREFERENCES AB Background: For > 100 years cattle production in the southern United States has been threatened by cattle fever. It is caused by an invasive parasite-vector complex that includes the protozoan hemoparasites Babesia bovis and B. bigemina, which are transmitted among domestic cattle via Rhipicephalus tick vectors of the subgenus Boophilus. In 1906 an eradication effort was started and by 1943 Boophilus ticks had been confined to a narrow tick eradication quarantine area (TEQA) along the Texas-Mexico border. However, a dramatic increase in tick infestations in areas outside the TEQA over the last decade suggests these tick vectors may be poised to re-invade the southern United States. We investigated historical and potential future distributions of climatic habitats of cattle fever ticks to assess the potential for a range expansion. Methods: We built robust spatial predictions of habitat suitability for the vector species Rhipicephalus (Boophilus) microplus and R. (B.) annulatus across the southern United States for three time periods: 1906, present day (2012), and 2050. We used analysis of molecular variance (AMOVA) to identify persistent tick occurrences and analysis of bias in the climate proximate to these occurrences to identify key environmental parameters associated with the ecology of both species. We then used ecological niche modeling algorithms GARP and Maxent to construct models that related known occurrences of ticks in the TEQA during 2001-2011 with geospatial data layers that summarized important climate parameters at all three time periods. Results: We identified persistent tick infestations and specific climate parameters that appear to be drivers of ecological niches of the two tick species. Spatial models projected onto climate data representative of climate in 1906 reproduced historical pre-eradication tick distributions. Present-day predictions, although constrained to areas near the TEQA, extrapolated well onto climate projections for 2050. Conclusions: Our models indicate the potential for range expansion of climate suitable for survival of R. microplus and R. annulatus in the southern United States by mid-century, which increases the risk of reintroduction of these ticks and cattle tick fever into major cattle producing areas. C1 [Giles, John R.; Busch, Joseph D.; Wagner, David M.] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA. [Peterson, A. Townsend] Univ Kansas, Biodivers Inst, Lawrence, KS 66045 USA. [Olafson, Pia U.; Pound, J. Mathews; Kammlah, Diane M.; Lohmeyer, Kimberly H.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Scoles, Glen A.] Washington State Univ, USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [Davey, Ronald B.] USDA ARS, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. RP Wagner, DM (reprint author), No Arizona Univ, Ctr Microbial Genet & Genom, POB 4073, Flagstaff, AZ 86011 USA. EM dave.wagner@nau.edu OI Peterson, A. Townsend/0000-0003-0243-2379 FU USDA-NIFA [2010-6510420386] FX We thank USDA-APHIS mounted patrol inspectors for collecting field samples used in this study. This work was supported by USDA-NIFA Grant 2010-6510420386. Use of trade, product, or firm names does not imply endorsement by the US Government. The USDA is an equal opportunity provider and employer. NR 88 TC 12 Z9 14 U1 4 U2 35 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD APR 17 PY 2014 VL 7 AR 189 DI 10.1186/1756-3305-7-189 PG 11 WC Parasitology SC Parasitology GA AF9ZF UT WOS:000335072500002 PM 24742062 ER PT J AU Corby-Harris, V Maes, P Anderson, KE AF Corby-Harris, Vanessa Maes, Patrick Anderson, Kirk E. TI The Bacterial Communities Associated with Honey Bee (Apis mellifera) Foragers SO PLOS ONE LA English DT Article ID RIBOSOMAL-RNA; GUT BACTERIA; POLLEN; WORKER; HEALTH; COLONY; MICROBIOLOGY; MICROFLORA; BREAD; PHYLLOSPHERE AB The honey bee is a key pollinator species in decline worldwide. As part of a commercial operation, bee colonies are exposed to a variety of agricultural ecosystems throughout the year and a multitude of environmental variables that may affect the microbial balance of individuals and the hive. While many recent studies support the idea of a core microbiota in guts of younger in-hive bees, it is unknown whether this core is present in forager bees or the pollen they carry back to the hive. Additionally, several studies hypothesize that the foregut (crop), a key interface between the pollination environment and hive food stores, contains a set of 13 lactic acid bacteria (LAB) that inoculate collected pollen and act in synergy to preserve pollen stores. Here, we used a combination of 454 based 16S rRNA gene sequencing of the microbial communities of forager guts, crops, and corbicular pollen and crop plate counts to show that (1) despite a very different diet, forager guts contain a core microbiota similar to that found in younger bees, (2) corbicular pollen contains a diverse community dominated by hive-specific, environmental or phyllosphere bacteria that are not prevalent in the gut or crop, and (3) the 13 LAB found in culture-based studies are not specific to the crop but are a small subset of midgut or hindgut specific bacteria identified in many recent 454 amplicon-based studies. The crop is dominated by Lactobacillus kunkeei, and Alpha 2.2 (Acetobacteraceae), highly osmotolerant and acid resistant bacteria found in stored pollen and honey. Crop taxa at low abundance include core hindgut bacteria in transit to their primary niche, and potential pathogens or food spoilage organisms seemingly vectored from the pollination environment. We conclude that the crop microbial environment is influenced by worker task, and may function in both decontamination and inoculation. C1 [Corby-Harris, Vanessa; Anderson, Kirk E.] USDA, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Maes, Patrick; Anderson, Kirk E.] Univ Arizona, Ctr Insect Sci, Tucson, AZ 85721 USA. RP Anderson, KE (reprint author), USDA, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. EM kirk.anderson@ars.usda.gov FU USA tax-payer supported operating budget of the ARS-USDA Carl Hayden Bee Research Center, Tucson AZ FX Funding was provided entirely by the USA tax-payer supported operating budget of the ARS-USDA Carl Hayden Bee Research Center, Tucson AZ. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. There were no current external funding sources for this study. NR 87 TC 30 Z9 30 U1 9 U2 78 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 16 PY 2014 VL 9 IS 4 AR e95056 DI 10.1371/journal.pone.0095056 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI4VW UT WOS:000336863900099 PM 24740297 ER PT J AU Matthews, BF Beard, H Brewer, E Kabir, S MacDonald, MH Youssef, RM AF Matthews, Benjamin F. Beard, Hunter Brewer, Eric Kabir, Sara MacDonald, Margaret H. Youssef, Reham M. TI Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5, confer partial resistance to soybean cyst nematode (Heterodera glycines) when overexpressed in transgenic soybean roots SO BMC PLANT BIOLOGY LA English DT Article DE Arabidopsis; Composite plants; Gene overexpression; Jasmonic acid; Resistance; Salicylic acid; Soybean; Soybean cyst nematode; Transgenic roots ID SYSTEMIC ACQUIRED-RESISTANCE; PROGRAMMED CELL-DEATH; COA-BINDING PROTEIN; LOCI UNDERLYING RESISTANCE; SALICYLIC-ACID INDUCTION; PLANT-DISEASE RESISTANCE; GATED ION-CHANNEL; DEFENSE RESPONSES; KNOT NEMATODE; SIGNALING PATHWAYS AB Background: Extensive studies using the model system Arabidopsis thaliana to elucidate plant defense signaling and pathway networks indicate that salicylic acid (SA) is the key hormone triggering the plant defense response against biotrophic and hemi-biotrophic pathogens, while jasmonic acid (JA) and derivatives are critical to the defense response against necrotrophic pathogens. Several reports demonstrate that SA limits nematode reproduction. Results: Here we translate knowledge gained from studies using Arabidopsis to soybean. The ability of thirty-one Arabidopsis genes encoding important components of SA and JA synthesis and signaling in conferring resistance to soybean cyst nematode (SCN: Heterodera glycines) are investigated. We demonstrate that overexpression of three of thirty-one Arabidoposis genes in transgenic soybean roots of composite plants decreased the number of cysts formed by SCN to less than 50% of those found on control roots, namely AtNPR1(33%), AtTGA2 (38%), and AtPR-5 (38%). Three additional Arabidopsis genes decreased the number of SCN cysts by 40% or more: AtACBP3 (53% of the control value), AtACD2 (55%), and AtCM-3 (57%). Other genes having less or no effect included AtEDS5 (77%), AtNDR1 (82%), AtEDS1 (107%), and AtPR-1 (80%), as compared to control. Overexpression of AtDND1 greatly increased susceptibility as indicated by a large increase in the number of SCN cysts (175% of control). Conclusions: Knowledge of the pathogen defense system gained from studies of the model system, Arabidopsis, can be directly translated to soybean through direct overexpression of Arabidopsis genes. When the genes, AtNPR1, AtGA2, and AtPR-5, encoding specific components involved in SA regulation, synthesis, and signaling, are overexpressed in soybean roots, resistance to SCN is enhanced. This demonstrates functional compatibility of some Arabidopsis genes with soybean and identifies genes that may be used to engineer resistance to nematodes. C1 [Matthews, Benjamin F.; Beard, Hunter; Brewer, Eric; Kabir, Sara; MacDonald, Margaret H.; Youssef, Reham M.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Youssef, Reham M.] Fayoum Univ, Al Fayyum, Egypt. RP Matthews, BF (reprint author), USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. EM ben.matthews@ars.usda.gov FU United Soybean Board [1254] FX The authors thank Dr. Leslie Wanner for critical review of the manuscript, Patrick Elias for DNA sequencing, Dr. Hua Lu for discussions, and Andrea Maldonado for cloning genes. Financial support from United Soybean Board no. 1254 is gratefully acknowledged. NR 158 TC 11 Z9 14 U1 3 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD APR 16 PY 2014 VL 14 AR 96 DI 10.1186/1471-2229-14-96 PG 19 WC Plant Sciences SC Plant Sciences GA AG3WV UT WOS:000335351500001 PM 24739302 ER PT J AU Williams, MR Buda, AR Elliott, HA Hamlett, J Boyer, EW Schmidt, JP AF Williams, Mark R. Buda, Anthony R. Elliott, Herschel A. Hamlett, James Boyer, Elizabeth W. Schmidt, John P. TI Groundwater flow path dynamics and nitrogen transport potential in the riparian zone of an agricultural headwater catchment SO JOURNAL OF HYDROLOGY LA English DT Article DE Nitrate; Riparian zone; Preferential flow; Groundwater seep; Nonpoint source pollution ID ATLANTIC COASTAL-PLAIN; NITRATE REMOVAL; HYDROLOGIC CONTROLS; ORGANIC-CARBON; STREAM; PATTERNS; FOREST; WATERSHEDS; LAND; DENITRIFICATION AB Shallow groundwater dynamics play a critical role in determining the chemistry and movement of nitrogen (N) in the riparian zone. In this study, we characterized N concentration variability and hydrologic transport pathways in shallow groundwater draining areas of a riparian area with and without emergent groundwater seeps. The study was conducted in FD36, an agricultural headwater catchment in the Ridge and Valley physiographic region of central Pennsylvania, USA. Three seep and adjacent non-seep areas were each instrumented with a field of 40 piezometers installed in a grid pattern (1.5-m spacing) at both 20- and 60-cm depths. Piezometers were monitored seasonally for approximately two years (October 2010-May 2012). Results showed that hydraulic head within seep areas was variable and some regions exhibited upward vertical hydraulic gradients of 0.18-0.27. Non-seep areas were characterized by uniform hydraulic head levels and were relatively hydrostatic. Nitrate-N (NO3-N) concentrations in seep areas were significantly greater than those in the non-seep areas at two of the three study sites. A two-component mixing model using chloride as a conservative tracer indicated that shallow groundwater in seep areas was primarily (53-75%) comprised of water from a shallow fractured aquifer, which had elevated NO3-N concentrations (5.7 mg L-1). Shallow groundwater in non-seep areas, however, was comprised (58-82%) of perched water on top of the fragipan that was likely recharged locally in the riparian zone and had low NO3-N concentrations (0.6 mg L-1). Higher NO3-N concentrations, variable hydraulic head, and groundwater emergence onto the land surface in seep areas provided evidence for preferential flow paths as an important conduit for water and N movement in these areas of the riparian zone. We conclude that the potential for N delivery to the stream in FD36 was much greater from seep areas compared to non-seep areas. Targeted management of seeps should be a priority in efforts to reduce NO3-N levels in riparian zones within headwater agricultural catchments. Published by Elsevier B.V. C1 [Williams, Mark R.; Elliott, Herschel A.; Hamlett, James] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. [Buda, Anthony R.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Boyer, Elizabeth W.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Schmidt, John P.] DuPont Pioneer, Champaign Res Ctr, Ivesdale, IL 61851 USA. RP Williams, MR (reprint author), USDA ARS, Soil Drainage Res Unit, 590 Woody Hayes Dr, Columbus, OH 43210 USA. EM mark.williams2@ars.usda.gov NR 49 TC 10 Z9 10 U1 2 U2 45 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD APR 16 PY 2014 VL 511 BP 870 EP 879 DI 10.1016/j.jhydrol.2014.02.033 PG 10 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AG2VJ UT WOS:000335274900075 ER PT J AU Engstrom, MT Palijarvi, M Fryganas, C Grabber, JH Mueller-Harvey, I Salminen, JP AF Engstrom, Marica T. Palijarvi, Maija Fryganas, Christos Grabber, John H. Mueller-Harvey, Irene Salminen, Juha-Pekka TI Rapid Qualitative and Quantitative Analyses of Proanthocyanidin Oligomers and Polymers by UPLC-MS/MS SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE proanthocyanidin; procyanidin; prodelphinidin; tannins; mass spectrometry; MRM ID PERFORMANCE LIQUID-CHROMATOGRAPHY; SAINFOIN ONOBRYCHIS-VICIIFOLIA; IONIZATION MASS-SPECTROMETRY; REVERSED-PHASE; CONDENSED TANNINS; PARASITIC NEMATODES; CATTLE NEMATODES; IN-VITRO; HPLC; PROCYANIDINS AB This paper presents the development of a rapid method with ultraperformance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) for the qualitative and quantitative analyses of plant proanthocyanidins directly from crude plant extracts. The method utilizes a range of cone voltages to achieve the depolymerization step in the ion source of both smaller oligomers and larger polymers. The formed depolymerization products are further fragmented in the collision cell to enable their selective detection. This UPLC-MS/MS method is able to separately quantitate the terminal and extension units of the most common proanthocyanidin subclasses, that is, procyanidins and prodelphinidins. The resulting data enable (1) quantitation of the total proanthocyanidin content, (2) quantitation of total procyanidins and prodelphinidins including the procyanidin/prodelphinidin ratio, (3) estimation of the mean degree of polymerization for the oligomers and polymers, and (4) estimation of how the different procyanidin and prodelphinidin types are distributed along the chromatographic hump typically produced by large proanthocyanidins. All of this is achieved within the 10 min period of analysis, which makes the presented method a significant addition to the chemistry tools currently available for the qualitative and quantitative analyses of complex proanthocyanidin mixtures from plant extracts. C1 [Engstrom, Marica T.; Palijarvi, Maija; Salminen, Juha-Pekka] Univ Turku, Dept Chem, Lab Organ Chem & Chem Biol, FI-20014 Turku, Finland. [Fryganas, Christos; Mueller-Harvey, Irene] Univ Reading, Chem & Biochem Lab, Food Prod & Qual Div, Sch Agr Policy & Dev, Reading RG6 6AT, Berks, England. [Grabber, John H.] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI 53706 USA. RP Engstrom, MT (reprint author), Univ Turku, Dept Chem, Lab Organ Chem & Chem Biol, FI-20014 Turku, Finland. EM marica.engstrom@utu.fi FU Turku University Foundation, Academy of Finland [258992]; University of Turku Graduate School (UTUGS); EU Marie Curie Initial Training Network [PITN-GA-2011-289377]; RS Specific Cooperative Agreement with University of Reading [58-3655-0-155F] FX This study was supported by Turku University Foundation, Academy of Finland (Grant 258992 to J.P.S.), the University of Turku Graduate School (UTUGS), an EU Marie Curie Initial Training Network (PITN-GA-2011-289377, "LegumePlus"), and an ARS Specific Cooperative Agreement with University of Reading (58-3655-0-155F). The Strategic Research Grant (Ecological Interactions) enabled the use of the UPLC-MS/MS instrument. NR 45 TC 18 Z9 18 U1 9 U2 63 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 16 PY 2014 VL 62 IS 15 BP 3390 EP 3399 DI 10.1021/jf500745y PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AF4CF UT WOS:000334658300010 PM 24665824 ER PT J AU Gates, MW AF Gates, Michael W. TI Nomenclatural Notes on the Eurytomids (Chalcidoidea: Eurytomidae) Described by Jean Brehes Housed in Museo Argentino de Ciencias Naturales "Bernardino Rivadavia" SO ZOOTAXA LA English DT Article DE lectotype; Argentina; Proseurytoma; Aximopsis; Eurytoma; Sycophila; Phylloxeroxenus; Tetramesa ID HYMENOPTERA AB Ten Eurytomidae (Hymenoptera) parasitic wasp species described by Jean Brethes and deposited in the Museo Argentino de Ciencias Naturales in Buenos Aires are treated and their nomenclature stabilized. The condition of the type material is described. Lectotypes are designated for Decatoma cecidosiphaga Brethes, Prodecatoma parodii Brethes, Eudecatoma opposita Brethes, and Eurytoma caridei Brethes. One new generic synonymy, Xanthosomodes Brethes with Tetramesa Walker, n. syn., and five new combinations are proposed: Tetramesa albiangulata (Brethes), n. comb.; Phylloxeroxenus caridei (Brethes), n. comb.; Aximopsis vulgata (Brethes), n. comb.; Proseurytoma parodii (Brethes), n. comb.; and Bruchophagus opposita (Brethes), n. comb. Sycophila paranensis Brethes is declared incertae sedis. C1 ARS, Systemat Entomol Lab, USDA, PSI,Natl Museum Nat Hist, Washington, DC 20013 USA. RP Gates, MW (reprint author), ARS, Systemat Entomol Lab, USDA, PSI,Natl Museum Nat Hist, Washington, DC 20013 USA. EM michael.gates@ars.usda.gov NR 21 TC 1 Z9 1 U1 0 U2 0 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD APR 16 PY 2014 VL 3790 IS 1 BP 185 EP 193 PG 9 WC Zoology SC Zoology GA AE9VO UT WOS:000334359500009 PM 24869892 ER PT J AU Meyer, RE Morrow, WEM Stikeleather, LF Baird, CL Rice, JM Byrne, H Halbert, BV Styles, DK AF Meyer, Robert E. Morrow, W. E. Morgan Stikeleather, Larry F. Baird, Craig L. Rice, J. Mark Byrne, Haleh Halbert, Burt V. Styles, Darrel K. TI Evaluation of carbon dioxide administration for on-site mass depopulation of swine in response to animal health emergencies SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Editorial Material ID WELFARE IMPLICATIONS; INHALATION ANESTHESIA; NEWBORN PIGLETS; MOUTH-DISEASE; PIGS; POULTRY; SLAUGHTER; EPIDEMIC; STRESS; FOOT C1 [Meyer, Robert E.] Mississippi State Univ, Coll Vet Med, Dept Clin Sci, Mississippi State, MS 39762 USA. [Morrow, W. E. Morgan] N Carolina State Univ, Dept Anim Sci, Coll Agr & Life Sci, Raleigh, NC 27607 USA. [Stikeleather, Larry F.; Baird, Craig L.; Rice, J. Mark; Halbert, Burt V.] N Carolina State Univ, Dept Biol & Agr Engn, Coll Agr & Life Sci, Raleigh, NC 27607 USA. [Byrne, Haleh] N Carolina State Univ, Ind & Extens Serv, Coll Agr & Life Sci, Raleigh, NC 27607 USA. [Styles, Darrel K.] USDA APHIS Vet Serv, Natl Ctr Anim Hlth Emergency Management, Riverdale, MD 20737 USA. RP Meyer, RE (reprint author), Mississippi State Univ, Coll Vet Med, Dept Clin Sci, Mississippi State, MS 39762 USA. EM meyer@cvm.msstate.edu NR 34 TC 1 Z9 1 U1 3 U2 5 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 EI 1943-569X J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD APR 15 PY 2014 VL 244 IS 8 BP 924 EP 933 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA CA6BW UT WOS:000348994400021 PM 24697768 ER PT J AU Rota, CT Millspaugh, JJ Rumble, MA Lehman, CP Kesler, DC AF Rota, Christopher T. Millspaugh, Joshua J. Rumble, Mark A. Lehman, Chad P. Kesler, Dylan C. TI The Role of Wildfire, Prescribed Fire, and Mountain Pine Beetle Infestations on the Population Dynamics of Black-Backed Woodpeckers in the Black Hills, South Dakota SO PLOS ONE LA English DT Article ID CAVITY-NESTING BIRDS; HABITAT ASSOCIATIONS; PICOIDES-ARCTICUS; BURNED FORESTS; BOREAL FOREST; COMMUNITIES; ABUNDANCE; USA; DISTURBANCES; SEVERITY AB Wildfire and mountain pine beetle infestations are naturally occurring disturbances in western North American forests. Black-backed woodpeckers (Picoides arcticus) are emblematic of the role these disturbances play in creating wildlife habitat, since they are strongly associated with recently-killed forests. However, management practices aimed at reducing the economic impact of natural disturbances can result in habitat loss for this species. Although black-backed woodpeckers occupy habitats created by wildfire, prescribed fire, and mountain pine beetle infestations, the relative value of these habitats remains unknown. We studied habitat-specific adult and juvenile survival probabilities and reproductive rates between April 2008 and August 2012 in the Black Hills, South Dakota. We estimated habitat-specific adult and juvenile survival probability with Bayesian multi-state models and habitat-specific reproductive success with Bayesian nest survival models. We calculated asymptotic population growth rates from estimated demographic rates with matrix projection models. Adult and juvenile survival and nest success were highest in habitat created by summer wildfire, intermediate in MPB infestations, and lowest in habitat created by fall prescribed fire. Mean posterior distributions of population growth rates indicated growing populations in habitat created by summer wildfire and declining populations in fall prescribed fire and mountain pine beetle infestations. Our finding that population growth rates were positive only in habitat created by summer wildfire underscores the need to maintain early post-wildfire habitat across the landscape. The lower growth rates in fall prescribed fire and MPB infestations may be attributed to differences in predator communities and food resources relative to summer wildfire. C1 [Rota, Christopher T.; Millspaugh, Joshua J.; Kesler, Dylan C.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. [Rumble, Mark A.] US Forest Serv, Forest & Grassland Res Lab, Rocky Mt Res Stn, Rapid City, SD USA. [Lehman, Chad P.] South Dakota Dept Game Fish & Pk, Custer, SD USA. RP Rota, CT (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO 65211 USA. EM rotact@missouri.edu OI Rota, Christopher/0000-0001-9272-4687 FU United States Forest Service Rocky Mountain Research Station [07-JV-11221609-211]; National Fire Plan [10-JV-11221632-178]; South Dakota Department of Game, Fish and Parks [08-CO-11221632-111]; South Dakota Department of Game, Fish, and Parks [T-39-R-1, 2439]; Missouri Chapter of the Wildlife Society; University of Missouri ORG; James D. Chambers Memorial Scholarship; Judy Southern Fellowship; TransWorld Airlines Scholarship FX Funding for this project was provided by the United States Forest Service Rocky Mountain Research Station agreement 07-JV-11221609-211 and the National Fire Plan agreement 10-JV-11221632-178. The South Dakota Department of Game, Fish and Parks provided funding through agreement number 08-CO-11221632-111 and the Wildlife Diversity Small Grants Program. This study was also made possible through State Wildlife Grant T-39-R-1, Study #2439, provided by the South Dakota Department of Game, Fish, and Parks. CTR was also supported by the Missouri Chapter of the Wildlife Society, the University of Missouri ORG, the James D. Chambers Memorial Scholarship, the Judy Southern Fellowship, and a TransWorld Airlines Scholarship administered by the University of Missouri. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 57 TC 9 Z9 11 U1 6 U2 38 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 15 PY 2014 VL 9 IS 4 AR e94700 DI 10.1371/journal.pone.0094700 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI5PW UT WOS:000336922600085 PM 24736502 ER PT J AU Perez-Suarez, M Castellano, MJ Kolka, R Asbjornsen, H Helmers, M AF Perez-Suarez, Marlin Castellano, Michael J. Kolka, Randall Asbjornsen, Heidi Helmers, Matthew TI Nitrogen and carbon dynamics in prairie vegetation strips across topographical gradients in mixed Central Iowa agroecosystems SO AGRICULTURE ECOSYSTEMS & ENVIRONMENT LA English DT Article DE Agriculture; C3:C4 ratio; Carbon; N cycling; Plant litter; Plant N uptake ID PLANT FUNCTIONAL COMPOSITION; GULF-OF-MEXICO; TALLGRASS PRAIRIE; FILTER STRIPS; SOIL CARBON; GRASSLAND SOILS; FIRE; PATTERNS; BUFFERS; IMMOBILIZATION AB Reductions of nitrogen (N) export from agricultural lands because of changes in specific N stocks and fluxes by incorporation of small amounts of prairie vegetation strips (PVS) are poorly understood. The primary objective of this study was to evaluate the effect of the presence and topographical position of PVS on soil and plant carbon (C) and N stocks relative to annual crop and native prairie vegetation. The study was implemented within three small adjacent watersheds, treated with one of the following cover types: (1) 100% row-crop agriculture (CROP); (2) 20% prairie vegetation (PVS) distributed along the contour across three topographical positions: upslope, sideslope and footslope position; and (3) 100% 17-year old reconstructed native prairie (RNP) as the control condition. Total soil organic C (SOC), total soil N (TN), inorganic N availability as indexed by ion exchange resins, N stocks in plant biomass and litter, and the ratio of C3:C4 plant species were measured during the 2010 growing season. Results showed that over five years of treatment, PVS footslope improved soil quality by increasing TN by almost 100% and SOC by 37%; while CROP footslope TN decreased by 31% and SOC decreased by 28%. Overall, N stocks in plant biomass and litter were higher in PVS compared with RNP, except in the footslope where the lower N plant stocks was associated with higher C3 abundance in RNP. Nitrogen availability was higher in CROP (25.4 +/- 1.4), followed by PVS (10.2 +/- 1.3), and RNP (2.2 +/- 1.4); with the highest values recorded in the upslope position for PVS and RNP, and the footslope for CROP. These findings are important for designing watersheds with PVS to reduce N accumulation in the footslope position and promote additional N retention in soil organic matter and plant biomass, thereby minimizing N losses to streams. (C) 2014 Elsevier B.V. All rights reserved. C1 [Perez-Suarez, Marlin] Iowa State Univ, Dept Nat Resource Ecol & Management, Ames, IA 50011 USA. [Perez-Suarez, Marlin] Univ Autonoma Estado Mexico, Inst Ciencias Agr & Rurales, Toluca 50000, Mexico. [Castellano, Michael J.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Kolka, Randall] USDA Forest Serv, No Res Stn, Grand Rapids, MN 06514 USA. [Asbjornsen, Heidi] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Helmers, Matthew] Iowa State Univ, Dept Agr & Biosyst Engn, Ames, IA 50011 USA. RP Asbjornsen, H (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. EM heidi.asbjornsen@unh.edu OI Castellano, Michael/0000-0003-1411-7931 FU USDA Grant [2010-85101-2046] FX The authors gratefully acknowledge the support of Pauline Drobney and the staff at the Neal Smith National Wildlife Refuge. Thanks to Christopher Witte for his assistance on field work. Funding for this project was in part provided by USDA Grant 2010-85101-20469. NR 56 TC 4 Z9 4 U1 4 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-8809 EI 1873-2305 J9 AGR ECOSYST ENVIRON JI Agric. Ecosyst. Environ. PD APR 15 PY 2014 VL 188 BP 1 EP 11 DI 10.1016/j.agee.2014.01.023 PG 11 WC Agriculture, Multidisciplinary; Ecology; Environmental Sciences SC Agriculture; Environmental Sciences & Ecology GA AI2PI UT WOS:000336700100001 ER PT J AU Choice, ZD Frazer, TK Jacoby, CA AF Choice, Zanethia D. Frazer, Thomas K. Jacoby, Charles A. TI Light requirements of seagrasses determined from historical records of light attenuation along the Gulf coast of peninsular Florida SO MARINE POLLUTION BULLETIN LA English DT Article DE Thalassia testudinum; Halodule wrightii; Syringodium filiforme; Halophila engelmannii; Water quality; Florida Gulf coast ID SUBMERGED AQUATIC VEGETATION; THALASSIA-TESTUDINUM; WATER-QUALITY; HALODULE-WRIGHTII; NUTRIENT ENRICHMENT; ECOSYSTEM SERVICES; DEPTH DISTRIBUTION; LAGOON SYSTEM; LAGUNA MADRE; GROWTH AB Seagrasses around the world are threatened by human activities that degrade water quality and reduce light availability. In this study, light requirements were determined for four common and abundant seagrasses along the Gulf coast of peninsular Florida using a threshold detecting algorithm. Light requirements ranged from 8% to 10% of surface irradiance for Halophila engelmannii to 25-27% of surface irradiance for Halodule wrightii. Requirements for all species differed from previous reports generated at other locations. Variations were attributed to morphological and physiological differences, as well as adaptation to light histories at specific locations. In addition, seagrasses were absent from stations with significantly higher concentrations of total nitrogen, total phosphorus, chlorophyll a and color. These results confirm the need to address links between increased anthropogenic nutrient loads, eutrophication, reduced light penetration, and loss of seagrasses and the services they provide. Published by Elsevier Ltd. C1 [Choice, Zanethia D.; Frazer, Thomas K.] Univ Florida, Sch Nat Resources & Environm, Gainesville, FL 32611 USA. [Frazer, Thomas K.] Univ Florida, Sch Forest Resources & Conservat, Fisheries & Aquat Sci Program, Gainesville, FL 32653 USA. [Jacoby, Charles A.] Univ Florida, Dept Soil & Water Sci, Gainesville, FL 32653 USA. RP Choice, ZD (reprint author), USDA Forest Serv, Ctr Bottom land & Hardwoods Res, Southern Res Stn, 1000 Front St, Oxford, MS 38655 USA. EM zdchoice@gmail.com; frazer@ufl.edu; cajacoby@ufl.edu FU Southwest Florida Water Management District; Suwannee River Water Management District; State Wildlife Grant from the Florida Fish and Wildlife Conservation Commission; School of Natural Resources and Environment at the University of Florida FX This work was supported in part by the Southwest Florida Water Management District, the Suwannee River Water Management District, a State Wildlife Grant from the Florida Fish and Wildlife Conservation Commission, and the School of Natural Resources and Environment at the University of Florida. These supporters played no role in designing or conducting the research or preparing and submitting the article. NR 63 TC 4 Z9 4 U1 6 U2 33 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0025-326X EI 1879-3363 J9 MAR POLLUT BULL JI Mar. Pollut. Bull. PD APR 15 PY 2014 VL 81 IS 1 BP 94 EP 102 DI 10.1016/j.marpolbul.2014.02.015 PG 9 WC Environmental Sciences; Marine & Freshwater Biology SC Environmental Sciences & Ecology; Marine & Freshwater Biology GA AG7VI UT WOS:000335626500024 PM 24613262 ER PT J AU Beh, MM Metz, MR Seybold, SJ Rizzo, DM AF Beh, Maia M. Metz, Margaret R. Seybold, Steven J. Rizzo, David M. TI The novel interaction between Phytophthora ramorum and wildfire elicits elevated ambrosia beetle landing rates on tanoak, Notholithocarpus densiflorus SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Anthaxia; Flatheaded borers; Gnathotrichus pilosus; Metallic woodboring beetles; Sudden oak death; Xyleborinus saxesenii ID SUDDEN OAK DEATH; COAST LIVE OAK; BARK BEETLES; SPECIES COMPOSITION; UNITED-STATES; CALIFORNIA; COLEOPTERA; DISEASE; PATHOGEN; ETHANOL AB The 2008 wildfires in the Big Sur region of California's central coast-the first to occur in forests impacted by Phytophthora ramorum, the non-native, invasive pathogen that causes sudden oak death-provided the rare opportunity to study the response of scolytid and other subcortical beetles to this novel disturbance interaction. We used sticky card traps attached to the main stem of tanoak, Notholithocarpus densiflorus, the tree species most susceptible to P. ramorum, to determine which subcortical beetle species may be using tanoak as a host and to compare insect landing rates on these trees in forest plots impacted by neither disturbance, either wildfire or P. ramorum disturbance alone, or both disturbances combined. Xyleborinus saxesenii and Gnathotrichus pilosus, two species of ambrosia beetles (Coleoptera: Scolytidae), composed the majority (48% and 40%, respectively) of subcortical beetles landing on tanoaks during both years of the study. Adults of two species of a small, branch-feeding flatheaded borer (Anthaxia sp.; Coleoptera: Buprestidae) were also captured in relative abundance landing on tanoaks in the combined disturbance plots during the second year of the study. All but two of the 2779 scolytid beetles collected in this study were trapped on tanoaks in forest plots disturbed by P. ramorum and/or fire, and 75% of these scolytids were trapped during the fall 2009 season. The majority of scolytids were trapped on tanoaks in plots containing both disturbances (81% in 2009 and 79% in 2010), and, of the two disturbances, more scolytids were trapped on tanoaks in burned plots than in P. ramorum-infested plots (92% more in 2009 and 476% more in 2010). Semiochemicals emanating from the tanoaks upon which the sticky cards were attached-either in the form of host volatile compounds or scolytid aggregation pheromones-presumably affected ambrosia beetle landing rates, and greater quantities of moribund and recently-killed trees in the plots disturbed both by P. ramorum and fire may have led to greater population densities of ambrosia beetles in these areas. Our findings of elevated ambrosia beetle landing rates in Big Sur forests with mixed disturbances suggest a heightened threat to tanoak in these areas, but additional research is needed to determine the actual frequency of ambrosia beetle gallery initiation in living tanoaks and whether colonization hastens or leads to tree mortality. (C) 2014 Elsevier B.V. All rights reserved. C1 [Beh, Maia M.; Metz, Margaret R.; Rizzo, David M.] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Seybold, Steven J.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95616 USA. RP Beh, MM (reprint author), Univ Calif Davis, Dept Plant Pathol, One Shields Ave, Davis, CA 95616 USA. EM mmbeh@ucdavis.edu; mrmetz@ucdavis.edu; sjseybold59@gmail.com; dmrizzo@ucdavis.edu FU USDA Forest Service Pacific Southwest Research Station, USDA Forest Service State and Private Forestry; National Science Foundation [EF-0622770]; Gordon and Betty Moore Foundation; Jastro Shields Graduate Student Research Fellowship; University of California, Davis FX The authors would like to thank Kerri Frangioso, Mark Readdie and Feynner Arias at the Landels-Hill Big Creek Reserve, the Monterey Peninsula Regional Park District, California State Parks, Los Padres National Forest, Don McQueen, and assorted members of the Rizzo lab for their assistance on this project. We also thank Yigen Chen, UC-Davis Department of Entomology, for his advice on the statistical treatment of the data, and Donald Bright, Colorado State University, Richard Westcott, Oregon Department of Agriculture, and Svatopluk Bily, National Museum, Prague, Czech Republic, for insect identifications. We are also grateful to Andrew Cline, California Department of Food and Agriculture, Sacramento, for assistance with retrieval of buprestid literature and providing access to the California State Collection of Arthropods. Funding was provided by USDA Forest Service Pacific Southwest Research Station, USDA Forest Service State and Private Forestry, the National Science Foundation (EF-0622770), and the Gordon and Betty Moore Foundation. Partial funding for this project came from a grant from the Jastro Shields Graduate Student Research Fellowship, University of California, Davis. NR 66 TC 0 Z9 0 U1 1 U2 21 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2014 VL 318 BP 21 EP 33 DI 10.1016/j.foreco.2014.01.007 PG 13 WC Forestry SC Forestry GA AE6EH UT WOS:000334082900003 ER PT J AU Kennedy, MC Johnson, MC AF Kennedy, Maureen C. Johnson, Morris C. TI Fuel treatment prescriptions alter spatial patterns of fire severity around the wildland-urban interface during the Wallow Fire, Arizona, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fuel treatment; Environmental management; Wallow Fire; Wild land-urban interface; Spatial analysis ID MIXED-CONIFER FOREST; UNITED-STATES; DRY FORESTS; MANAGEMENT; WILDFIRE; PINE; CALIFORNIA; PONDEROSA; WASHINGTON; PRINCIPLES AB Fuel reduction treatments are implemented in the forest surrounding the wildland-urban interface (WUI) to provide defensible space and safe opportunity for the protection of homes during a wildfire. The 2011 Wallow Fire in Arizona USA burned through recently implemented fuel treatments in the wildland surrounding residential communities in the WUI, and those fuel treatments have been credited with providing firefighter opportunities to protect residences during the Wallow Fire and thereby preventing the loss of homes that otherwise would have been burned. To characterize the spatial pattern of fire severity (represented by crown scorch and bole char) as the fire entered the treated areas from the wildland we fit non-linear models to the relationship between each severity metric and distance from the treatment edge in the direction of fire spread. The non-linear curve we chose provides an estimate of the distance into the treated area at which the severity metric is substantially reduced. Fire severity as measured by crown scorch and bole char was reduced a greater distance into the fuel treatment that allowed for clumps of trees and buffers for wildlife habitat than for the fuel treatment that resulted in evenly distributed trees with complete removal of ladder fuels. Crown scorch persisted further into the treated areas than did bole char, which implies that a high intensity surface fire was maintained in the treated areas. All of the fuel treatments we studied in the Wallow Fire demonstrated reduced fire severity before encountering residences in the WUI, demonstrating that there are multiple paths to fuel treatment design around the WUI. (C) 2014 Elsevier B.V. All rights reserved. C1 [Kennedy, Maureen C.] Univ Washington, Seattle, WA 98195 USA. [Johnson, Morris C.] US Forest Serv, USDA, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Kennedy, MC (reprint author), Univ Washington, Box 352100, Seattle, WA 98195 USA. EM mkenn@uw.edu; mcjohnson@fs.fed.us FU U.S. Forest Service Pacific Northwest Research Station and Western Wildlands Environmental Threat Assessment Center FX Miguel Cruz, Dave Peterson, E. David Ford and Donald McKenzie provided thoughtful comments that improved early drafts of this manuscript. Two anonymous reviewers helped in clarifying the scope and strengthening the inference of the study. Joe Restaino, Jon Dvorak, Bob Vihnanek, Brooke Cassell, and Conamara Burke conducted field work for this project. Jim Pitts, Robert Whitten, Rusty Bigelow organized site visits on the Wallow Fire and Rob Lever, Stephanie Coleman, Raymond Rugg, and Jim Schroeder provided information on the Wallow Fire. Stacey Weaver, Nancy Loving, and Patti O'Connor provided GIS data and support, and Rob Norheim supplied the maps in the manuscript. Brent Wachter provided fire weather data and fire photos. Richard Davalos, Pamela Bostwick, and Sue Sitko gave assisted with the data, and Maria Petrova provided database management. This project was funded by the U.S. Forest Service Pacific Northwest Research Station and Western Wildlands Environmental Threat Assessment Center. NR 48 TC 17 Z9 17 U1 4 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2014 VL 318 BP 122 EP 132 DI 10.1016/j.foreco.2014.01.014 PG 11 WC Forestry SC Forestry GA AE6EH UT WOS:000334082900013 ER PT J AU Perhans, K Haight, RG Gustafsson, L AF Perhans, Karin Haight, Robert G. Gustafsson, Lena TI The value of information in conservation planning: Selecting retention trees for lichen conservation SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Biodiversity; Cost-effectiveness; Epiphyte; Forestry; Information cost; Sweden ID RESERVE SELECTION; FOREST; DIVERSITY; RESPONSES; INVESTMENT; EPIPHYTES; SOUTHERN; BOREAL; RETURN; COSTS AB Conservation planning studies at small scales such as forest stands and below are uncommon. However, for retention forestry, developed during the last two decades and with current wide and increasing application in boreal and temperate regions, the need for cost-effective selection of individual trees is evident. In retention forestry certain trees are left at final harvest to promote flora and fauna. There is also a scarcity of studies on information costs and how these relate to the cost-effectiveness of conservation. We addressed both of these issues by studying whether decisions about the retention of aspen Populus tremula L. trees can be made more cost-effectively by including information about tree characteristics. We analyzed data from 12 recently harvested stands in middle Sweden containing 131 epiphytic lichen species (a biodiversity proxy) on 360 aspen trees. We related the presence of lichen species to bark and stem attributes and used those relationships to prioritize trees for retention. We estimated the value of using different sets of survey information (lichens, tree characteristics) to select retention trees to achieve various conservation goals. Depending on species or species groups of interest, and the type of tree information being collected, the value of collecting the information is up to 20% of the total value of all potential retention trees, which, given current labor costs, allows up to four hours for planning and selecting the right trees on an average-sized clearcut. The current practice of almost randomly selecting aspen trees to retain at final harvest can be improved by adding easily collected information on tree characteristics, such as black-colored bark, slow tree growth, inclining stems and speckled bark. This can lead to attainment of a given level of a conservation goal (like maximizing the number of lichen species of conservation concern) with fewer retention trees. Inventory of tree information often can be performed quickly, and if part of the gains from using such information to guide tree selection would be spent on additional conservation efforts, this would benefit biodiversity. Studies on more organism groups and tree species are needed to increase the applicability of results. (C) 2014 The Authors. Published by Elsevier B.V. C1 [Perhans, Karin; Gustafsson, Lena] Swedish Univ Agr Sci, Dept Ecol, SE-75007 Uppsala, Sweden. [Haight, Robert G.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP Gustafsson, L (reprint author), Swedish Univ Agr Sci, Dept Ecol, POB 7044, SE-75007 Uppsala, Sweden. EM karin.perhans@gmail.com; rhaight@fs.fed.us; lena.gustafsson@slu.se FU Swedish Research Council Formas [215-2009-569] FX We are grateful to Fredrik Jonsson who performed the lichen survey and registered aspen characteristics, to Johanna Lundstrom who assisted with the selection of survey stands, and to Malin Johansson who helped collecting the data on aspen characteristics. We thank Johanna Lundstrom and Stephanie Snyder for valuable comments on the manuscript. The project was funded by The Swedish Research Council Formas (Grant no. 215-2009-569 to L. Gustafsson). NR 42 TC 3 Z9 3 U1 1 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 15 PY 2014 VL 318 BP 175 EP 182 DI 10.1016/j.foreco.2014.01.020 PG 8 WC Forestry SC Forestry GA AE6EH UT WOS:000334082900019 ER PT J AU Selling, GW Utt, KD AF Selling, Gordon W. Utt, Kelly D. TI Improved Zein Films Using Polyethylenemaleic Anhydride SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE crosslinking; biopolymers and renewable polymers; films; proteins; mechanical properties ID CROSS-LINKING REAGENT; CHEMICAL-MODIFICATION; SUCCINIC ANHYDRIDE; PLANT-PROTEINS; ACETIC-ACID; CORN ZEIN; FIBERS; GLYOXAL; GLUTARALDEHYDE; TEMPERATURE AB The effect on the physical properties and solubility of corn protein (zein) films was studied after reaction of zein with polyethylenemaleic anhydride (PEMA). Reactions were carried out in dimethylformamide (DMF) solution where the concentration of PEMA was varied between 0 and 6%. After reaction at room temperature, cast films were prepared, and the physical and solubility properties were determined. Incorporating more than 2% PEMA provided films with increased tensile strength and elongation. Incorporating 6% PEMA provided films with higher tensile strength (32-42 MPa) and improved solvent resistance (100-21% solubility) relative to control. If the films were heated in an oven, the film's solubility decreased further. Solution rheology experiments demonstrated that the zein and PEMA formed a cross-linked gel with time in DMF. Dynamic mechanical analysis experiments have shown that the PEMA-modified zein films undergo a dramatic loss in modulus on reaching 116 degrees C, whereas the control experiences this loss at 98 degrees C. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40122. C1 [Selling, Gordon W.; Utt, Kelly D.] ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Selling, GW (reprint author), ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM gordon.selling@ars.usda.gov NR 44 TC 2 Z9 2 U1 3 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD APR 15 PY 2014 VL 131 IS 8 AR 40122 DI 10.1002/app.40122 PG 5 WC Polymer Science SC Polymer Science GA AE2MC UT WOS:000333806100024 ER PT J AU Wang, Y Stevens, VL Shah, R Peterson, JJ Dwyer, JT Gapstur, SM McCullough, ML AF Wang, Ying Stevens, Victoria L. Shah, Roma Peterson, Julia J. Dwyer, Johanna T. Gapstur, Susan M. McCullough, Marjorie L. TI Dietary Flavonoid and Proanthocyanidin Intakes and Prostate Cancer Risk in a Prospective Cohort of US Men SO AMERICAN JOURNAL OF EPIDEMIOLOGY LA English DT Article DE flavonoids; proanthocyanidins; prospective cohort study; prostate cancer ID II NUTRITION COHORT; JAPANESE MEN; ISOFLAVONE CONSUMPTION; PHYSICAL-ACTIVITY; TEA CONSUMPTION; UNITED-STATES; PREVENTION; SOY; QUESTIONNAIRE; COMPILATION AB Higher dietary intakes of flavonoids and proanthocyanidins have been associated with a lower risk of several cancers. Few prospective epidemiologic studies have examined individual flavonoids and proanthocyanidins in relation to prostate cancer. We examined these associations in a prospective US cohort of 43,268 men with a mean age of 70 years who completed detailed self-administered questionnaires in 19992000. During a mean follow-up of 7.8 years, 3,974 total prostate cancers, including 567 high-grade cases and 362 advanced cases, were ascertained. Cox proportional hazards regression models were used to calculate multivariable-adjusted relative risks and 95 confidence intervals. Residual energy-adjusted total flavonoids (for fifth quintile vs. first quintile, relative risk 1.11, 95 confidence interval: 1.01, 1.23; P for trend 0.02) and several subclasses were positively associated with overall prostate cancer risk, mostly limited to the top quintile and the first 2 years of follow-up. The associations for total flavonoids, flavan-3-ols, and proanthocyanidins with high-grade prostate cancer risk varied by follow-up time. During follow-up from 2002 to 2009, we observed suggestive inverse trends with higher total flavonoids (P for trend 0.05) and proanthocyanidins (P for trend 0.04) with high-grade prostate cancer, but not with advanced prostate cancer. Although evidence is limited, a possible role of total flavonoids and proanthocyanidins in prostate cancer tumor progression deserves further study. C1 [Wang, Ying; Stevens, Victoria L.; Shah, Roma; Gapstur, Susan M.; McCullough, Marjorie L.] Amer Canc Soc, Epidemiol Res Program, Atlanta, GA 30303 USA. [Peterson, Julia J.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer United States Dept Agr, Human Nutr Ctr Aging, Boston, MA 02111 USA. RP Wang, Y (reprint author), Amer Canc Soc, Epidemiol Res Program, 6D-222,250 Williams St NW, Atlanta, GA 30303 USA. EM ying.wang@cancer.org RI Wang, Ying/K-4705-2014; OI Wang, Ying/0000-0002-1241-6252; Dwyer, Johanna/0000-0002-0783-1769 FU US Department of Agriculture, Agricultural Research Service [58-1950-7-707]; American Cancer Society FX This work was supported in part with resources from the US Department of Agriculture, Agricultural Research Service (agreement 58-1950-7-707). The American Cancer Society funds the creation, maintenance, and updating of the Cancer Prevention Study-II (CPS-II) cohort. NR 51 TC 12 Z9 12 U1 0 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9262 EI 1476-6256 J9 AM J EPIDEMIOL JI Am. J. Epidemiol. PD APR 15 PY 2014 VL 179 IS 8 BP 974 EP 986 DI 10.1093/aje/kwu006 PG 13 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AE6CI UT WOS:000334075700006 PM 24567173 ER PT J AU Schilling, KE Gassman, PW Kling, CL Campbell, T Jha, MK Wolter, CF Arnold, JG AF Schilling, Keith E. Gassman, Philip W. Kling, Catherine L. Campbell, Todd Jha, Manoj K. Wolter, Calvin F. Arnold, Jeffrey G. TI The potential for agricultural land use change to reduce flood risk in a large watershed SO HYDROLOGICAL PROCESSES LA English DT Article DE flood; SWAT; land use change; flood risk; watershed modelling ID RACCOON RIVER; ASSESSMENT-TOOL; RUNOFF GENERATION; CATCHMENT; MANAGEMENT; IMPACT; SOIL; WALES; SWAT; IOWA AB Effects of agricultural land management practices on surface runoff are evident at local scales, but evidence for watershed-scale impacts is limited. In this study, we used the Soil and Water Assessment Tool model to assess changes in downstream flood risks under different land uses for the large, intensely agricultural, Raccoon River watershed in Iowa. We first developed a baseline model for flood risk based on current land use and typical weather patterns and then simulated the effects of varying levels of increased perennials on the landscape under the same weather patterns. Results suggest that land use changes in the Raccoon River could reduce the likelihood of flood events, decreasing both the number of flood events and the frequency of severe floods. The duration of flood events were not substantially affected by land use change in our assessment. The greatest flood risk reduction was associated with converting all cropland to perennial vegetation, but we found that converting half of the land to perennial vegetation or extended rotations (and leaving the remaining area in cropland) could also have major effects on reducing downstream flooding potential. We discuss the potential costs of adopting the land use change in the watershed to illustrate the scale of subsidies required to induce large-scale conversion to perennially based systems needed for flood risk reduction. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Schilling, Keith E.; Wolter, Calvin F.] Geol & Water Survey, Iowa Dept Nat Resources, Iowa City, IA 52242 USA. [Gassman, Philip W.; Kling, Catherine L.; Campbell, Todd] Iowa State Univ, Dept Econ, Ctr Agr & Rural Dev, Ames, IA USA. [Jha, Manoj K.] North Carolina A&T Univ, Civil Architectural & Environm Engn Dept, Greensboro, NC USA. [Arnold, Jeffrey G.] ARS, USDA, Grassland Soil & Water Res Lab, Temple, TX USA. RP Schilling, KE (reprint author), Geol & Water Survey, Iowa Dept Nat Resources, Iowa City, IA 52242 USA. EM keith.schilling@dnr.iowa.gov OI Kling, Catherine L/0000-0002-4785-7154 FU University of Iowa, Iowa Flood Center [Q 1000822048]; US Department of Agriculture, National Institute of Food and Agriculture [20116800530411] FX This research was partially funded by the University of Iowa, Iowa Flood Center, Award No. Q 1000822048, Impact of Land Use and Climate change on Flood Risk and Ecosystem Services for Major Iowa Watersheds and by the US Department of Agriculture, National Institute of Food and Agriculture, Award No. 20116800530411, Agro-ecosystem Approach to Sustainable Biofuels Production via the Pyrolysis-Biochar Platform (AFRI-CAP). NR 51 TC 9 Z9 11 U1 6 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD APR 15 PY 2014 VL 28 IS 8 BP 3314 EP 3325 DI 10.1002/hyp.9865 PG 12 WC Water Resources SC Water Resources GA AE0VA UT WOS:000333683100009 ER PT J AU Kim, D Oren, R Oishi, AC Hsieh, CI Phillips, N Novick, KA Stoy, PC AF Kim, Dohyoung Oren, Ram Oishi, A. Christopher Hsieh, Cheng-I Phillips, Nathan Novick, Kimberly A. Stoy, Paul C. TI Sensitivity of stand transpiration to wind velocity in a mixed broadleaved deciduous forest SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Aerodynamic resistance; Canopy resistance; Sap flux density; Stomatal resistance; Transpiration; Wind velocity ID BOUNDARY-LAYER CONDUCTANCE; SAP-FLOW MEASUREMENTS; SOIL-WATER AVAILABILITY; VAPOR-PRESSURE DEFICIT; LEAF-AREA INDEX; STOMATAL CONDUCTANCE; CANOPY CONDUCTANCE; PINE FOREST; CARBON ASSIMILATION; APPARENT RESPONSES AB Wind velocity (U) within and above forest canopies can alter the coupling between the vapor-saturated sub-stomatal airspace and the drier atmosphere aloft, thereby influencing transpiration rates. In practice, however, the actual increase in transpiration with increasing U depends on the aerodynamic resistance (R-A) to vapor transfer compared to canopy resistance to water vapor flux out of leaves (R-C, dominated by stomatal resistance, R-stom), and the rate at which R-A decreases with increasing U. We investigated the effect of U on transpiration at the canopy scale using filtered meteorological data and sap flux measurements gathered from six diverse species of a mature broadleaved deciduous forest. Only under high light conditions, stand transpiration (E-C) increased slightly (6.5%) with increasing U ranging from 0.7 to similar to 4.7 ms(-1). Under other conditions, sap flux density (J(s)) and E-C responded weakly or did not change with U. R-A, estimated from Monin-Obukhov similarity theory, decreased with increasing U, but this decline was offset by increasing R-C, estimated from a rearranged Penman-Monteith equation, due to a concurrent increase in vapor pressure deficit (D). The increase of R-C with D over the observed range of U was consistent with increased R-stom by similar to 40% based on hydraulic theory. Except for very rare half-hourly values, the proportion of R-A to total resistance (R-T) remained <15% over the observed range of conditions. These results suggest that in similar forests and conditions, the direct effect of U reducing R-A and thus increasing transpiration is negligible. However, the observed U-D relationship and its effect on R-stom must be considered when modeling canopy photosynthesis. (C) 2013 Elsevier B.V. All rights reserved. C1 [Kim, Dohyoung; Oren, Ram; Oishi, A. Christopher] Duke Univ, Nicholas Sch Environm, Durham, NC 27708 USA. [Oren, Ram] Swedish Univ Agr Sci SLU, Dept Forest Ecol & Management, SE-90183 Umea, Sweden. [Hsieh, Cheng-I] Natl Taiwan Univ, Dept Bioenvironm Syst Engn, Taipei 10617, Taiwan. [Phillips, Nathan] Boston Univ, Dept Geog & Environm, Boston, MA 02215 USA. [Novick, Kimberly A.] Indiana Univ, Sch Publ & Environm Affairs, Bloomington, IN 47405 USA. [Stoy, Paul C.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Oishi, A. Christopher] US Forest Serv, USDA, Southern Res Stn, Coweeta Hydrol Lab, Otto, NC 28763 USA. RP Kim, D (reprint author), Duke Univ, Nicholas Sch Environm, Box 90328, Durham, NC 27708 USA. EM dohyoung.kim@duke.edu FU Office of Science (BER) of the US Department of Energy through the Southeastern Regional Center (SERC) of the National Institute for Global Environmental Change (NIGEC); Office of Science (BER) of the US Department of Energy through Terrestrial Carbon Processes (TCP) program FX This research was supported by the Office of Science (BER) of the US Department of Energy through the Southeastern Regional Center (SERC) of the National Institute for Global Environmental Change (NIGEC), and through Terrestrial Carbon Processes (TCP) program. NR 73 TC 12 Z9 13 U1 8 U2 98 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD APR 15 PY 2014 VL 187 BP 62 EP 71 DI 10.1016/j.agrformet.2013.11.013 PG 10 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AB0NJ UT WOS:000331489100006 ER PT J AU Bosch, DD Marshall, LK Teskey, R AF Bosch, David D. Marshall, Laura. K. Teskey, Robert TI Forest transpiration from sap flux density measurements in a Southeastern Coastal Plain riparian buffer system SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Transpiration; Sap flow; Groundwater; Riparian forest ID WATER-USE; UNITED-STATES; THERMAL DISSIPATION; CLIMATE-CHANGE; FLOW; TREES; VELOCITY; EVAPOTRANSPIRATION; STREAMFLOW; IMPACTS AB Forested riparian buffers are prevalent throughout the Southeastern Coastal Plain Region of the United States (US). Vegetation in the riparian forest buffers in this region are primarily made up of slash pine (Pinus elliottii Engelm.), longleaf pine (Pinus palustris Mill.), and yellow poplar (Liriodendron tulipifera L.) trees. Because they make up a significant portion of the regional landscape, transpiration within these riparian buffers is believed to have an important impact on the hydrologic budget of regional watersheds. A riparian buffer along a first order stream in South-central Georgia US was selected for a sap flow study designed to provide measurements of tree transpiration. The forest provided a buffer zone that averaged 70 m in width from an upland field to the first order stream. Shallow water table conditions allowed direct interaction between the tree's root system and groundwater. Sap flux density, groundwater, and climatic data were collected to determine transpiration rates from different tree species and their relationship to potential ET rates and hydrologic and environmental conditions. Average sap flow rates ranged from 2 to 142 L day(-1). Sap flow was related to tree diameter, solar flux density, and daily vapor pressure deficit. An exponential relationship was developed that related measured average daily sap flow rate (L day(-1)) to tree diameter at breast height (DBH) (mm) for the range of measured trees (56 < DBH < 390 mm) with a coefficient of 2.04 and an exponent of 0.01. On an area basis, the average transpiration for the studied 720 m(2) study area was 1114 mm for the observation period from April to December. This represented 103% of the potential evapotranspiration for a reference grass (PET) for that same period. While landscape position, and subsequently access to groundwater, did not appear to strongly influence sap flow rates, reduced soil water in the vadose zone led to reductions in tree transpiration. The data indicate that transpiration within regional buffers uses a disproportionate amount of water on a per area basis compared to upland land covers, an important consideration when examining overall water consumption in regional watersheds. Published by Elsevier B.V. C1 [Bosch, David D.] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. [Marshall, Laura. K.] Ohio Environm Protect Agcy, Southwest Dist Off, Dayton, OH 45402 USA. [Teskey, Robert] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Bosch, DD (reprint author), USDA ARS, SE Watershed Res Lab, 2316 Rainwater Rd,POB 748, Tifton, GA 31793 USA. EM david.bosch@ars.usda.gov; laura.marshall@epa.ohio.gov; rteskey@uga.edu NR 43 TC 8 Z9 8 U1 5 U2 57 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD APR 15 PY 2014 VL 187 BP 72 EP 82 DI 10.1016/j.agrformet.2013.12.002 PG 11 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AB0NJ UT WOS:000331489100007 ER PT J AU Sansone-Land, A Takeoka, GR Shoemaker, CF AF Sansone-Land, Angelina Takeoka, Gary R. Shoemaker, Charles F. TI Volatile constituents of commercial imported and domestic black-ripe table olives (Olea europaea) SO FOOD CHEMISTRY LA English DT Article DE Olea europaea; Black-ripe table olives; Volatiles ID TOMATO PASTE VOLATILES; ODOR THRESHOLDS; DILUTION ANALYSIS; AROMA COMPONENTS; KEY ODORANTS; IDENTIFICATION; PRODUCTS; FLAVOR; CHROMATOGRAPHY; QUALITIES AB Volatile constituents of commercial black-ripe table olives (Olea europaea) from the United States, Spain, Egypt and Morocco were analysed by gas chromatography and gas chromatography-mass spectrometry (GC-MS). Dynamic headspace sampling was used to isolate a variety of aldehydes, alcohols, esters, ketones, phenols, terpenes, norisoprenoids, and pyridines. Odour unit values, calculated from concentration and odour threshold data, indicate that the following compounds are major contributors to black-ripe table olive aroma: B-damascenone, nonanal, (E)-dec-2-enal, 3-methylbutanal, ethyl benzoate, octanal, 2-methoxyphenol, 2-methylbutanal and 2-methoxy-4-methylphenol. Imported olives contained a variety of fermentation derived volatiles that were not detected in domestic olives. Constituents such as ethyl 2-methylbutanoate, ethyl 3-methylbutanoate, 3-methylbutyl acetate, oct-1-en-3-one, ethyl hexanoate, (Z)-hex-3-enyl acetate, hexyl acetate, ethyl cyclohexanecarboxylate, benzyl acetate and 4-ethylphenol contributed to the odour of imported olives but were not detected in domestic olives. (C) 2013 Published by Elsevier Ltd. C1 [Sansone-Land, Angelina; Takeoka, Gary R.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Sansone-Land, Angelina; Shoemaker, Charles F.] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. RP Takeoka, GR (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM asansoneland@gmail.com; gary.takeoka@ars.usda.gov; cfshoemaker@ucdavis.edu FU California Olive Committee (COC) FX This research was supported by the California Olive Committee (COC). We express gratitude to Dan Flynn (UC Davis Olive Center) for his assistance. NR 40 TC 9 Z9 10 U1 0 U2 59 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD APR 15 PY 2014 VL 149 BP 285 EP 295 DI 10.1016/j.foodchem.2013.10.090 PG 11 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA 287PC UT WOS:000329553700040 PM 24295708 ER PT J AU Munoz-Amatriain, M Cuesta-Marcos, A Endelman, JB Comadran, J Bonman, JM Bockelman, HE Chao, SM Russell, J Waugh, R Hayes, PM Muehlbauer, GJ AF Munoz-Amatriain, Maria Cuesta-Marcos, Alfonso Endelman, Jeffrey B. Comadran, Jordi Bonman, John M. Bockelman, Harold E. Chao, Shiaoman Russell, Joanne Waugh, Robbie Hayes, Patrick M. Muehlbauer, Gary J. TI The USDA Barley Core Collection: Genetic Diversity, Population Structure, and Potential for Genome-Wide Association Studies SO PLOS ONE LA English DT Article ID SMALL GRAINS COLLECTION; HORDEUM-VULGARE L.; QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; BREEDING GERMPLASM; CULTIVATED BARLEY; CROP IMPROVEMENT; GROWTH HABIT; BETA-GLUCAN; RESISTANCE AB New sources of genetic diversity must be incorporated into plant breeding programs if they are to continue increasing grain yield and quality, and tolerance to abiotic and biotic stresses. Germplasm collections provide a source of genetic and phenotypic diversity, but characterization of these resources is required to increase their utility for breeding programs. We used a barley SNP iSelect platform with 7,842 SNPs to genotype 2,417 barley accessions sampled from the USDA National Small Grains Collection of 33,176 accessions. Most of the accessions in this core collection are categorized as landraces or cultivars/breeding lines and were obtained from more than 100 countries. Both STRUCTURE and principal component analysis identified five major subpopulations within the core collection, mainly differentiated by geographical origin and spike row number (an inflorescence architecture trait). Different patterns of linkage disequilibrium (LD) were found across the barley genome and many regions of high LD contained traits involved in domestication and breeding selection. The genotype data were used to define 'mini-core' sets of accessions capturing the majority of the allelic diversity present in the core collection. These 'mini-core' sets can be used for evaluating traits that are difficult or expensive to score. Genome-wide association studies (GWAS) of 'hull cover', 'spike row number', and 'heading date' demonstrate the utility of the core collection for locating genetic factors determining important phenotypes. The GWAS results were referenced to a new barley consensus map containing 5,665 SNPs. Our results demonstrate that GWAS and high-density SNP genotyping are effective tools for plant breeders interested in accessing genetic diversity in large germplasm collections. C1 [Munoz-Amatriain, Maria; Muehlbauer, Gary J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Cuesta-Marcos, Alfonso; Hayes, Patrick M.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [Endelman, Jeffrey B.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Comadran, Jordi; Russell, Joanne; Waugh, Robbie] James Hutton Inst, Dundee, Scotland. [Bonman, John M.; Bockelman, Harold E.] ARS, USDA, Small Grains & Potato Germplasm Res Unit, Aberdeen, ID USA. [Chao, Shiaoman] ARS, USDA, Biosci Res Lab, Fargo, ND USA. [Muehlbauer, Gary J.] Univ Minnesota, Dept Plant Biol, St Paul, MN USA. RP Muehlbauer, GJ (reprint author), Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. EM muehl003@umn.edu FU Triticeae Coordinated Agricultural Project (TCAP) [2011-68002-30029]; United States Department of Agriculture - National Institute of Food and Agriculture (USDA-NIFA) FX This work was supported by the Triticeae Coordinated Agricultural Project (TCAP), grant no. 2011-68002-30029, funded by the United States Department of Agriculture - National Institute of Food and Agriculture (USDA-NIFA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 79 TC 39 Z9 43 U1 3 U2 74 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 14 PY 2014 VL 9 IS 4 AR e94688 DI 10.1371/journal.pone.0094688 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI6GP UT WOS:000336970400074 PM 24732668 ER PT J AU Langewisch, T Zhang, HX Vincent, R Joshi, T Xu, D Bilyeu, K AF Langewisch, Tiffany Zhang, Hongxin Vincent, Ryan Joshi, Trupti Xu, Dong Bilyeu, Kristin TI Major Soybean Maturity Gene Haplotypes Revealed by SNPViz Analysis of 72 Sequenced Soybean Genomes SO PLOS ONE LA English DT Article ID MAP-BASED CLONING; GROWTH HABIT; SENSITIVITY; SELECTION AB In this Genomics Era, vast amounts of next-generation sequencing data have become publicly available for multiple genomes across hundreds of species. Analyses of these large-scale datasets can become cumbersome, especially when comparing nucleotide polymorphisms across many samples within a dataset and among different datasets or organisms. To facilitate the exploration of allelic variation and diversity, we have developed and deployed an in-house computer software to categorize and visualize these haplotypes. The SNPViz software enables users to analyze region-specific haplotypes from single nucleotide polymorphism (SNP) datasets for different sequenced genomes. The examination of allelic variation and diversity of important soybean [Glycine max (L.) Merr.] flowering time and maturity genes may provide additional insight into flowering time regulation and enhance researchers' ability to target soybean breeding for particular environments. For this study, we utilized two available soybean genomic datasets for a total of 72 soybean genotypes encompassing cultivars, landraces, and the wild species Glycine soja. The major soybean maturity genes E1, E2, E3, and E4 along with the Dt1 gene for plant growth architecture were analyzed in an effort to determine the number of major haplotypes for each gene, to evaluate the consistency of the haplotypes with characterized variant alleles, and to identify evidence of artificial selection. The results indicated classification of a small number of predominant haplogroups for each gene and important insights into possible allelic diversity for each gene within the context of known causative mutations. The software has both a stand-alone and web-based version and can be used to analyze other genes, examine additional soybean datasets, and view similar genome sequence and SNP datasets from other species. C1 [Langewisch, Tiffany; Bilyeu, Kristin] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Zhang, Hongxin; Joshi, Trupti; Xu, Dong] Univ Missouri, Dept Comp Sci, Columbia, MO USA. [Vincent, Ryan] McKendree Univ, Div Comp, Lebanon, IL USA. [Joshi, Trupti; Xu, Dong] Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO USA. [Joshi, Trupti; Xu, Dong] Univ Missouri, Inst Informat, Columbia, MO USA. [Joshi, Trupti; Xu, Dong] Univ Missouri, Christopher S Bond Life Sci Ctr, Columbia, MO USA. RP Bilyeu, K (reprint author), Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. EM Kristin.Bilyeu@ars.usda.gov FU United Soybean Board; Missouri Soybean Merchandising Council FX Research was supported by a grant to KB by The United Soybean Board, and a grant to DX from the Missouri Soybean Merchandising Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 25 TC 9 Z9 10 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 11 PY 2014 VL 9 IS 4 AR e94150 DI 10.1371/journal.pone.0094150 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI3CI UT WOS:000336736200041 PM 24727730 ER PT J AU Herrmann, J Ravilious, GE McKinney, SE Westfall, CS Lee, SG Baraniecka, P Giovannetti, M Kopriva, S Krishnan, HB Jez, JM AF Herrmann, Jonathan Ravilious, Geoffrey E. McKinney, Samuel E. Westfall, Corey S. Lee, Soon Goo Baraniecka, Patrycja Giovannetti, Marco Kopriva, Stanislav Krishnan, Hari B. Jez, Joseph M. TI Structure and Mechanism of Soybean ATP Sulfurylase and the Committed Step in Plant Sulfur Assimilation SO JOURNAL OF BIOLOGICAL CHEMISTRY LA English DT Article DE Enzyme Mechanisms; Enzyme Structure; Plant; Plant Biochemistry; Protein Structure ID ADENOSINE 5'-PHOSPHOSULFATE KINASE; ARABIDOPSIS-THALIANA; CRYSTAL-STRUCTURE; SULFATE ASSIMILATION; ADENOSINE-5'-PHOSPHOSULFATE KINASE; NUCLEOTIDE-BINDING; ESCHERICHIA-COLI; 5'-ADENYLYLSULFATE REDUCTASE; SACCHAROMYCES-CEREVISIAE; PENICILLIUM-CHRYSOGENUM AB Background: ATP sulfurylase catalyzes the energetically unfavorable formation of adenosine 5-phosphosulfate in plant sulfur assimilation. Results: Structural and kinetic analyses identifies key active site residues. Conclusion: A reaction mechanism involving distortion of nucleotide conformation and stabilizing interactions is proposed. Significance: These results provide the first molecular insights on a plant ATP sulfurylase and the committed step of plant sulfur assimilation. Enzymes of the sulfur assimilation pathway are potential targets for improving nutrient content and environmental stress responses in plants. The committed step in this pathway is catalyzed by ATP sulfurylase, which synthesizes adenosine 5-phosphosulfate (APS) from sulfate and ATP. To better understand the molecular basis of this energetically unfavorable reaction, the x-ray crystal structure of ATP sulfurylase isoform 1 from soybean (Glycine max ATP sulfurylase) in complex with APS was determined. This structure revealed several highly conserved substrate-binding motifs in the active site and a distinct dimerization interface compared with other ATP sulfurylases but was similar to mammalian 3-phosphoadenosine 5-phosphosulfate synthetase. Steady-state kinetic analysis of 20 G. max ATP sulfurylase point mutants suggests a reaction mechanism in which nucleophilic attack by sulfate on the -phosphate of ATP involves transition state stabilization by Arg-248, Asn-249, His-255, and Arg-349. The structure and kinetic analysis suggest that ATP sulfurylase overcomes the energetic barrier of APS synthesis by distorting nucleotide structure and identifies critical residues for catalysis. Mutations that alter sulfate assimilation in Arabidopsis were mapped to the structure, which provides a molecular basis for understanding their effects on the sulfur assimilation pathway. C1 [Herrmann, Jonathan; Ravilious, Geoffrey E.; McKinney, Samuel E.; Westfall, Corey S.; Lee, Soon Goo; Jez, Joseph M.] Washington Univ, Dept Biol, St Louis, MO 63130 USA. [Baraniecka, Patrycja; Giovannetti, Marco; Kopriva, Stanislav] John Innes Ctr, Norwich NR4 7UH, Norfolk, England. [Giovannetti, Marco] Univ Turin, Dept Life Sci & Syst Biol, I-10125 Turin, Italy. [Krishnan, Hari B.] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. RP Jez, JM (reprint author), Washington Univ, Dept Biol, One Brookings Dr,Campus Box 1137, St Louis, MO 63130 USA. EM jjez@biology2.wustl.edu RI Kopriva, Stanislav/C-7401-2013; Giovannetti, Marco/J-8625-2015 OI Kopriva, Stanislav/0000-0002-7416-6551; Giovannetti, Marco/0000-0003-4637-9880 FU Department of Energy Office of Biological and Environmental Research [DE-AC02-06CH11357] FX Portions of this research were carried out at the Argonne National Laboratory Structural Biology Center of the Advanced Photon Source, a national user facility operated by the University of Chicago for the Department of Energy Office of Biological and Environmental Research and supported by Grant DE-AC02-06CH11357. Product names are necessary to report factually on available data; however, the United States Department of Agriculture neither guarantees nor warrants the standard of product, and the use of the name by the United States Department of Agriculture implies no approval of the product to the exclusion of others that may be suitable. NR 69 TC 12 Z9 12 U1 2 U2 15 PU AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA SN 0021-9258 EI 1083-351X J9 J BIOL CHEM JI J. Biol. Chem. PD APR 11 PY 2014 VL 289 IS 15 BP 10919 EP 10929 DI 10.1074/jbc.M113.540401 PG 11 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AE8CI UT WOS:000334226400059 PM 24584934 ER PT J AU Crosby, FL Wamsley, HL Pate, MG Lundgren, AM Noh, SM Munderloh, UG Barbet, AF AF Crosby, Francy L. Wamsley, Heather L. Pate, Melanie G. Lundgren, Anna M. Noh, Susan M. Munderloh, Ulrike G. Barbet, Anthony F. TI Knockout of an outer membrane protein operon of Anaplasma marginale by transposon mutagenesis SO BMC GENOMICS LA English DT Article ID TICK CELL-CULTURE; CD4+ T-LYMPHOCYTE; RICKETTSIA-RICKETTSII; GENETIC MANIPULATION; HOMOLOGOUS CHALLENGE; BORRELIA-BURGDORFERI; IDENTIFICATION; ANTIGENS; VACCINE; CATTLE AB Background: The large amounts of data generated by genomics, transcriptomics and proteomics have increased our understanding of the biology of Anaplasma marginale. However, these data have also led to new assumptions that require testing, ideally through classical genetic mutation. One example is the definition of genes associated with virulence. Here we describe the molecular characterization of a red fluorescent and spectinomycin and streptomycin resistant A. marginale mutant generated by Himar1 transposon mutagenesis. Results: High throughput genome sequencing to determine the Himar1-A. marginale genome junctions established that the transposon sequences were integrated within the coding region of the omp10 gene. This gene is arranged within an operon with AM1225 at the 5' end and with omp9, omp8, omp7 and omp6 arranged in tandem at the 3' end. RNA analysis to determine the effects of the transposon insertion on the expression of omp10 and downstream genes revealed that the Himar1 insertion not only reduced the expression of omp10 but also that of downstream genes. Transcript expression from omp9, and omp8 dropped by more than 90% in comparison with their counterparts in wild-type A. marginale. Immunoblot analysis showed a reduction in the production of Omp9 protein in these mutants compared to wild-type A. marginale. Conclusions: These results demonstrate that transposon mutagenesis in A. marginale is possible and that this technology can be used for the creation of insertional gene knockouts that can be evaluated in natural host-vector systems. C1 [Crosby, Francy L.; Lundgren, Anna M.; Barbet, Anthony F.] Univ Florida, Coll Vet Med, Dept Infect Dis & Pathol, 2015 SW 16th Ave, Gainesville, FL 32610 USA. [Wamsley, Heather L.; Pate, Melanie G.] Physiol Sci, Gainesville, FL 32610 USA. [Noh, Susan M.] USDA ARS, Anim Dis Res Unit, Pullman, WA 99164 USA. [Munderloh, Ulrike G.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. RP Crosby, FL (reprint author), Univ Florida, Coll Vet Med, Dept Infect Dis & Pathol, 2015 SW 16th Ave, Gainesville, FL 32610 USA. EM crosbyl@ufl.edu FU Wellcome Trust [GR075800M] FX This work received support from grant number GR075800M from the Wellcome Trust. NR 57 TC 5 Z9 5 U1 0 U2 1 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 11 PY 2014 VL 15 AR 278 DI 10.1186/1471-2164-15-278 PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA V42DV UT WOS:000209595400001 PM 24725301 ER PT J AU Villarino, GH Bombarely, A Giovannoni, JJ Scanlon, MJ Mattson, NS AF Villarino, Gonzalo H. Bombarely, Aureliano Giovannoni, James J. Scanlon, Michael J. Mattson, Neil S. TI Transcriptomic Analysis of Petunia hybrida in Response to Salt Stress Using High Throughput RNA Sequencing SO PLOS ONE LA English DT Article ID SEQ DATA; DIFFERENTIAL EXPRESSION; ROOT TRANSCRIPTOME; REFERENCE GENOME; ABIOTIC STRESS; TOMATO; IDENTIFICATION; GENES; TOLERANCE; PLANTS AB Salinity and drought stress are the primary cause of crop losses worldwide. In sodic saline soils sodium chloride (NaCl) disrupts normal plant growth and development. The complex interactions of plant systems with abiotic stress have made RNA sequencing a more holistic and appealing approach to study transcriptome level responses in a single cell and/or tissue. In this work, we determined the Petunia transcriptome response to NaCl stress by sequencing leaf samples and assembling 196 million Illumina reads with Trinity software. Using our reference transcriptome we identified more than 7,000 genes that were differentially expressed within 24 h of acute NaCl stress. The proposed transcriptome can also be used as an excellent tool for biological and bioinformatics in the absence of an available Petunia genome and it is available at the SOL Genomics Network (SGN) http://solgenomics.net. Genes related to regulation of reactive oxygen species, transport, and signal transductions as well as novel and undescribed transcripts were among those differentially expressed in response to salt stress. The candidate genes identified in this study can be applied as markers for breeding or to genetically engineer plants to enhance salt tolerance. Gene Ontology analyses indicated that most of the NaCl damage happened at 24 h inducing genotoxicity, affecting transport and organelles due to the high concentration of Na+ ions. Finally, we report a modification to the library preparation protocol whereby cDNA samples were bar-coded with non-HPLC purified primers, without affecting the quality and quantity of the RNA-seq data. The methodological improvement presented here could substantially reduce the cost of sample preparation for future high-throughput RNA sequencing experiments. C1 [Villarino, Gonzalo H.; Mattson, Neil S.] Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. [Bombarely, Aureliano; Scanlon, Michael J.] Cornell Univ, Dept Plant Biol, Ithaca, NY USA. [Bombarely, Aureliano; Giovannoni, James J.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Giovannoni, James J.] USDA ARS, Robert W Holley Res Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Villarino, GH (reprint author), Cornell Univ, Dept Hort, Ithaca, NY 14853 USA. EM ghv8@cornell.edu RI Bombarely, Aureliano/C-6580-2009 OI Bombarely, Aureliano/0000-0001-6257-8914 FU Post Schenkel Foundation FX This study was funded by an internal grant (Post Schenkel Foundation). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 46 TC 13 Z9 13 U1 5 U2 62 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 10 PY 2014 VL 9 IS 4 AR e94651 DI 10.1371/journal.pone.0094651 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AI5LS UT WOS:000336909100116 PM 24722556 ER PT J AU Kondo, MC Bream, KDW Barg, FK Branas, CC AF Kondo, Michelle C. Bream, Kent D. W. Barg, Frances K. Branas, Charles C. TI A random spatial sampling method in a rural developing nation SO BMC PUBLIC HEALTH LA English DT Article DE Geographic random sampling; Social surveys; Geographic information system (GIS); Global positioning system (GPS); Satellite imagery; Guatemala ID DESIGN; MORTALITY; PROGRAM AB Background: Nonrandom sampling of populations in developing nations has limitations and can inaccurately estimate health phenomena, especially among hard-to-reach populations such as rural residents. However, random sampling of rural populations in developing nations can be challenged by incomplete enumeration of the base population. Methods: We describe a stratified random sampling method using geographical information system (GIS) software and global positioning system (GPS) technology for application in a health survey in a rural region of Guatemala, as well as a qualitative study of the enumeration process. Results: This method offers an alternative sampling technique that could reduce opportunities for bias in household selection compared to cluster methods. However, its use is subject to issues surrounding survey preparation, technological limitations and in-the-field household selection. Application of this method in remote areas will raise challenges surrounding the boundary delineation process, use and translation of satellite imagery between GIS and GPS, and household selection at each survey point in varying field conditions. This method favors household selection in denser urban areas and in new residential developments. Conclusions: Random spatial sampling methodology can be used to survey a random sample of population in a remote region of a developing nation. Although this method should be further validated and compared with more established methods to determine its utility in social survey applications, it shows promise for use in developing nations with resource-challenged environments where detailed geographic and human census data are less available. C1 [Kondo, Michelle C.] USDA, Forest Serv, No Res Stn, Philadelphia, PA 19103 USA. [Kondo, Michelle C.; Branas, Charles C.] Univ Penn, Perelman Sch Med, Dept Biostat & Epidemiol, Philadelphia, PA 19104 USA. [Bream, Kent D. W.; Barg, Frances K.] Univ Penn, Perelman Sch Med, Dept Family Med & Community Hlth, Philadelphia, PA 19104 USA. RP Kondo, MC (reprint author), USDA, Forest Serv, No Res Stn, 100 North 20th St Suite 205, Philadelphia, PA 19103 USA. EM michelleckondo@fs.fed.us OI Kondo, Michelle/0000-0001-9152-1589 FU Office of the Provost, University of Pennsylvania; USDA-Forest Service Northern Research Station; Penn Genome Frontiers Institute; Pennsylvania Department of Health; University of Pennsylvania's interdisciplinary Guatemala Health Initiative FX We would like to thank Michael Broder, Leah Geoghegan, Ellen Kim, Natalie Ramirez, Chanelle Campbell, Ebony Easley, and Dominique Prue for their work carrying out the surveys. Summer Research Internships for students were supported by the Office of the Provost, University of Pennsylvania. We owe gratitude to staff at the Centro de Salud and Hospitalito Atitlan, and especially the Mayor and the municipality and the people of Santiago Atitlan for their generous support. This work was also supported in part by the USDA-Forest Service Northern Research Station, the Penn Genome Frontiers Institute and a grant with the Pennsylvania Department of Health. The Department of Health specifically disclaims responsibility for any analyses, interpretations, or conclusions. This work was conducted under the auspices of the University of Pennsylvania's interdisciplinary Guatemala Health Initiative. NR 22 TC 2 Z9 2 U1 1 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD APR 10 PY 2014 VL 14 AR 338 DI 10.1186/1471-2458-14-338 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AG6AK UT WOS:000335500500001 PM 24716473 ER PT J AU Kimball, BA Opiekun, M Yamazaki, K Beauchamp, GK AF Kimball, Bruce A. Opiekun, Maryanne Yamazaki, Kunio Beauchamp, Gary K. TI Immunization alters body odor SO PHYSIOLOGY & BEHAVIOR LA English DT Article DE Behavior; Innate immunity; Lipopolysaccharide; Vaccine; Volatiles ID MAJOR HISTOCOMPATIBILITY COMPLEX; FEMALE MICE; RABIES VACCINES; INDIVIDUAL RECOGNITION; INFLAMMATION; VACCINATION; COMMUNICATION; CONSPECIFICS; SIGNATURES; AVOIDANCE AB Infections have been shown to alter body odor. Because immune activation accompanies both infection and immunization, we tested the hypothesis that classical immunization might similarly result in the alteration of body odors detectable by trained biosensor mice. Using a Y-maze, we trained biosensor mice to distinguish between urine odors from rabies-vaccinated (RV) and unvaccinated control mice. RV-trained mice generalized this training to mice immunized with the equine West Nile virus (WNV) vaccine compared with urine of corresponding controls. These results suggest that there are similarities between body odors of mice immunized with these two vaccines. This conclusion was reinforced when mice could not be trained to directly discriminate between urine odors of RV- versus WNV-treated mice. Next, we trained biosensor mice to discriminate the urine odors of mice treated with lipopolysaccharide (LPS; a general elicitor of innate immunological responses) from the urine of control mice. These LPS-trained biosensors could distinguish between the odors of LPS-treated mouse urine and RV-treated mouse urine. Finally, biosensor mice trained to distinguish between the odors of RV-treated mouse urine and control mouse urine did not generalize this training to discriminate between the odors of LPS-treated mouse urine and control mouse urine. From these experiments, we conclude that: (1) immunization alters urine odor in similar ways for RV and WNV immunizations; and (2) immune activation with LPS also alters urine odor but in ways different from those of RV and WNV. Published by Elsevier Inc. C1 [Kimball, Bruce A.] Natl Wildlife Res Ctr, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, Philadelphia, PA 19104 USA. [Opiekun, Maryanne; Yamazaki, Kunio; Beauchamp, Gary K.] Monell Chem Senses Ctr, Philadelphia, PA 19104 USA. RP Kimball, BA (reprint author), Natl Wildlife Res Ctr, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, 3500 Market St, Philadelphia, PA 19104 USA. EM bruce.a.kimball@aphis.usda.gov; mopiekun@monell.org; beauchamp@monell.org FU USDA-APHIS-WS; Monell Chemical Senses Center (Monell); National Wildlife Research Center; USAMRMC-TATRC [W81XWH-12-2-0081] FX The contributions of Talia Martin with the behavioral assays are greatly appreciated. Funding for this research was made possible by a cooperative agreement between the USDA-APHIS-WS and the Monell Chemical Senses Center (Monell). MO, KY, and GKB thank Dr. Thomas Deliberto and the National Wildlife Research Center for their interest and financial support. USAMRMC-TATRC grant #W81XWH-12-2-0081 also made this work possible (GKB). Comments on previous versions of this manuscript from Dr. Jon Yewdell, Dr. Mike Tordoff, Dr. Hong Wang, and Dennis Kohler were particularly helpful. Mention of specific products does not constitute endorsement by either the USDA or Monell. NR 39 TC 5 Z9 5 U1 5 U2 12 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0031-9384 J9 PHYSIOL BEHAV JI Physiol. Behav. PD APR 10 PY 2014 VL 128 BP 80 EP 85 DI 10.1016/j.physbeh.2014.01.022 PG 6 WC Psychology, Biological; Behavioral Sciences SC Psychology; Behavioral Sciences GA AG0LE UT WOS:000335106200012 PM 24524972 ER PT J AU Yang, YX Wang, Y Zhou, K Hong, A AF Yang, Yuxin Wang, Yi Zhou, Kai Hong, An TI Constructing regulatory networks to identify biomarkers for insulin resistance SO GENE LA English DT Article DE Regulatory impact factor; Co-expression; Transcription factor; Microarray ID ESTROGEN-RECEPTOR-ALPHA; ENDOTHELIAL PROGENITOR CELLS; PANCREATIC BETA-CELLS; C-MYC EXPRESSION; ANDROGEN RECEPTOR; TRANSCRIPTIONAL REGULATION; CARDIOVASCULAR-DISEASE; DIABETES-MELLITUS; INDUCED APOPTOSIS; GENE-EXPRESSION AB Insulin resistance (IR) is a physiological condition in which cells fail to respond to the insulin hormone. Despite advances in the diagnosis and treatment of IR, novel molecular targets are still needed to improve the accuracy of diagnosis and the outcomes of therapy. Here, we present a systems approach to identify molecular biomarkers for IR We downloaded the gene expression profile of IR from the Gene Expression Omnibus (CEO), generated a regulatory network by mapping co-expressed genes to transcription factors (TFs) and calculated the regulatory impact factor of each transcription factor. Finally, we selected a list of potential molecular targets that could be used as therapeutic targets or diagnostic biomarkers, including ETS1, AR, ESR1 and Myc. Our studies identified multiple TFs that could play an important role in the pathogenesis of IR and provided a systems understanding of the potential relationships among these genes. Our study has the potential to aid in the understanding of IR and provides a basis for IR biomarker discovery. (c) 2014 The Authors. Published by Elsevier B.V. C1 [Yang, Yuxin; Hong, An] Jinan Univ, Coll Life Sci & Technol, Guangzhou 510632, Guangdong, Peoples R China. [Wang, Yi] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Zhou, Kai] Luzhou Med Coll, Affiliated Hosp, Luzhou 646000, Sichuan, Peoples R China. RP Hong, A (reprint author), Jinan Univ, Coll Life Sci & Technol, 601 Huangpu Blvd, Guangzhou 510632, Guangdong, Peoples R China. EM tha@jnu.edu.cn NR 56 TC 4 Z9 4 U1 1 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1119 EI 1879-0038 J9 GENE JI Gene PD APR 10 PY 2014 VL 539 IS 1 BP 68 EP 74 DI 10.1016/j.gene.2014.01.061 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA AD8DK UT WOS:000333496500010 PM 24512691 ER PT J AU Diaz, JA Moon, RJ Youngblood, JP AF Diaz, Jairo A. Moon, Robert J. Youngblood, Jeffrey P. TI Contrast Enhanced Microscopy Digital Image Correlation: A General Method to Contact-Free Coefficient of Thermal Expansion Measurement of Polymer Films SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE polymer films; phase contrast; polarized light; image analysis; coefficient of thermal expansion; digital image correlation ID ATOMIC-FORCE MICROSCOPY; SPECKLE PATTERN INTERFEROMETRY; THIN-FILMS; QUALITY ASSESSMENT; DEFORMATION; SURFACE; BLENDS; SCALE AB Thermal expansion represents a vital indicator of the processing history and dimensional stability of materials. Solvent-sensitive, thin, and compliant samples are particularly challenging to test. Here we describe how textures highlighted by contrast enhanced optical microscopy modes (i.e., polarized light (PL), phase contrast (PC)) and bright field (BF) can be used to determine the thermal expansion of polymer films in a contact-free way using digital image correlation (DIC). Three different films were explored: polyetherimide (PEI), polyimide (PI), and polyethylene naphthalate (PEN). Image textural features (e.g., intensity, size, speckle pattern characteristics) obtained by BF, PC, and PL were analyzed by two-dimensional Fourier transform and autocorrelations. The measured in-plane CTEs of PEI, PI, and PEN films, 51.8, 20.5, and 10.2 ppm/K, respectively, closely approached those previously reported using DIC with artificially applied speckle patterns. C1 [Diaz, Jairo A.; Youngblood, Jeffrey P.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. RP Youngblood, JP (reprint author), Purdue Univ, Sch Mat Engn, 701 West Stadium Ave, W Lafayette, IN 47907 USA. EM jpyoungb@purdue.edu FU Air Force Office of Scientific Research [FA9550-11-1-0162] FX The authors would like to thank the Air Force Office of Scientific Research grant #FA9550-11-1-0162 for supporting this project, and Prof. Carlos Martinez (Purdue University) for valuable discussions. NR 50 TC 2 Z9 2 U1 3 U2 37 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD APR 9 PY 2014 VL 6 IS 7 BP 4856 EP 4863 DI 10.1021/am405860y PG 8 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA AF2WI UT WOS:000334572800032 PM 24650286 ER PT J AU Chauhan, KR Schmidt, W AF Chauhan, Kamlesh R. Schmidt, Walter TI Biorational synthesis of iridomyrmecin diastereomers from catnip oil SO TETRAHEDRON LETTERS LA English DT Article DE Iridomyrmecin; Nepetalactone; Semiochemicals ID CHRYSOPA-OCULATA; PHEROMONE AB 4S,4aS,7S,7aR; 4R,4aS,7S,7aR; 4S,4aS,7S,7aS, and 4R,4aS,7S,7aS diastereomers of iridomyrmecin have been prepared in 5 steps from 4aS,7S,7aR and 4aS,7S,7aS-nepetalactones, major components of catnip oil. 4S,4aS,7S,7aR and 4R,4aS,7S,7aR-iridomyrmecin have been identified as defensive compounds from Iridomyrmex ants. Published by Elsevier Ltd. C1 [Chauhan, Kamlesh R.] USDA ARS, Invas Insect Biocontrol & Behav Lab, BARC West, Beltsville, MD 20705 USA. [Schmidt, Walter] BARC West, Environm Qual Lab, Beltsville, MD 20705 USA. RP Chauhan, KR (reprint author), USDA ARS, Invas Insect Biocontrol & Behav Lab, BARC West, B-007, Beltsville, MD 20705 USA. EM kamal.chauhan@ars.usda.gov FU Deployed War Fighter Protection Program FX We thank Ms. Ute Klingebiel, NMR Facility, USDA-ARS, Beltsville, Maryland, for her timely help in NMR spectroscopy and stereochemical assignments. We are grateful to funding provided by Deployed War Fighter Protection Program. NR 17 TC 0 Z9 0 U1 2 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0040-4039 J9 TETRAHEDRON LETT JI Tetrahedron Lett. PD APR 9 PY 2014 VL 55 IS 15 BP 2534 EP 2536 DI 10.1016/j.tetlet.2014.03.030 PG 3 WC Chemistry, Organic SC Chemistry GA AF8PC UT WOS:000334977100050 ER PT J AU Sapozhnikova, Y Byrdwell, WC Lobato, A Romig, B AF Sapozhnikova, Yelena Byrdwell, William Craig Lobato, Amada Romig, Bill TI Effects of UV-B Radiation Levels on Concentrations of Phytosterols, Ergothioneine, and Polyphenolic Compounds in Mushroom Powders Used As Dietary Supplements SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE mushroom powder; dietary supplement; phytosterols; polyphenolics; ergothioneine; UV-B radiation ID TANDEM MASS-SPECTROMETRY; EDIBLE MUSHROOMS; CULTIVATED MUSHROOMS; CHEMICAL-COMPOSITION; PHENOLIC-COMPOUNDS; STEROL COMPOSITION; UNITED-STATES; VITAMIN-D; HEALTH; IRRADIATION AB Compositional changes of powder dietary supplements made from mushrooms exposed to different levels of UV-B irradiation were evaluated for the bioactive naturally occurring mushroom antioxidant, ergothioneine; other natural polyphenolic compounds, e.g., flavonoids, lignans, etc.; and selected phytosterols. Four types of mushroom powder consisting of white, brown (Agaricus bisporus), oyster (Pleurotus ostreatus), and shiitake (Lentinula edodes) mushrooms from three different treatment groups (control, low and high UV-B exposures) were evaluated. Ergothioneine concentrations found in mushroom powders were 0.4-10.4 mg/g dry weight (dw) and were not appreciably affected by UV-B radiation. No individual polyphenols were detected above 0.1 mu g/g. Phytosterols ergosterol (2.4-6.2 mg/g dw) and campesterol (14-43 mu g/g dw) were measured in mushroom powder samples. Ergosterol concentrations decreased significantly with the increased level of UV-B treatment for all mushroom powder types, except for white. These results provide some new information on effects of UV-B radiation on these important natural bioactive compounds in mushrooms. C1 [Sapozhnikova, Yelena] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. [Byrdwell, William Craig] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. [Lobato, Amada] Monterey Mushrooms Inc, Watsonville, CA 95076 USA. [Romig, Bill] Midatlantic Perishable Commod Serv, Townsend, DE 19734 USA. RP Sapozhnikova, Y (reprint author), ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM yelena.sapozhnikova@ars.usda.gov OI Byrdwell, William/0000-0001-8241-428X FU Monterey Mushrooms, Inc. [293-1935-289] FX The authors acknowledge the Monterey Mushrooms, Inc., for providing funding for this research (Trust Fund #293-1935-289). NR 34 TC 5 Z9 6 U1 2 U2 33 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 9 PY 2014 VL 62 IS 14 BP 3034 EP 3042 DI 10.1021/jf403852k PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AF2WF UT WOS:000334572500002 ER PT J AU Rasooly, R Hernlem, B He, XH Friedman, M AF Rasooly, Reuven Hernlem, Bradley He, Xiaohua Friedman, Mendel TI Microwave Heating Inactivates Shiga Toxin (Stx2) in Reconstituted Fat-Free Milk and Adversely Affects the Nutritional Value of Cell Culture Medium SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE milk; Shiga toxin 2 (Stx2); microwave heating inactivation; cell culture; food safety ID ESCHERICHIA-COLI; FOOD; INHIBITION; QUALITY AB Microwave exposure is a convenient and widely used method for defrosting, heating, and cooking numerous foods. Microwave cooking is also reported to kill pathogenic microorganisms that often contaminate food. In this study, we tested whether microwaves would inactivate the toxicity of Shiga toxin 2 (Sta2) added to 5% reconstituted fat-free milk administered to monkey kidney Vero cells. Heating of milk spiked with Stx2 in a microwave oven using a 10% duty cycle (cycle period of 30 s) for a total of 165 kJ energy or thermal heating (pasteurization), widely used to kill pathogenic bacteria, did not destroy the biological effect of the toxin in the Vero cells. However, conventional heating of milk to 95 degrees C for 5 min or at an increased microwave energy of 198 kJ reduced the Stx2 activity. Gel electrophoresis showed that exposure of the protein toxin to high-energy microwaves resulted in the degradation of its original structure. In addition, two independent assays showed that exposure of the cell culture medium to microwave energy of 198 kJ completely destroyed the nutritional value of the culture medium used to grow the Vero cells, possibly by damaging susceptible essential nutrients present in the medium. These observations suggest that microwave heating has the potential to destroy the Shiga toxin in liquid food. C1 [Rasooly, Reuven; Hernlem, Bradley; He, Xiaohua] ARS, Foodborne Toxin Detect & Prevent Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Friedman, Mendel] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Rasooly, R (reprint author), ARS, Foodborne Toxin Detect & Prevent Unit, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM reuven.rasooly@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 NR 15 TC 3 Z9 3 U1 3 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD APR 9 PY 2014 VL 62 IS 14 BP 3301 EP 3305 DI 10.1021/jf500278a PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AF2WF UT WOS:000334572500035 ER PT J AU Choi, MY Kohler, R Meer, RKV Neupert, S Predel, R AF Choi, Man-Yeon Koehler, Rene Meer, Robert K. Vander Neupert, Susanne Predel, Reinhard TI Identification and Expression of Capa Gene in the Fire Ant, Solenopsis invicta SO PLOS ONE LA English DT Article ID MANDUCA-SEXTA ALLATOTROPIN; AMERICAN COCKROACH; DROSOPHILA-MELANOGASTER; PERISYMPATHETIC ORGANS; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; PROTEIN HORMONES; TOBACCO HAWKMOTH; NERVOUS-SYSTEM; CLEAVAGE SITES AB Recent genome analyses suggested the absence of a number of neuropeptide genes in ants. One of the apparently missing genes was the capa gene. Capa gene expression in insects is typically associated with the neuroendocrine system of abdominal ganglia; mature CAPA peptides are known to regulate diuresis and visceral muscle contraction. The apparent absence of the capa gene raised questions about possible compensation of these functions. In this study, we re-examined this controversial issue and searched for a potentially unrecognized capa gene in the fire ant, Solenopsis invicta. We employed a combination of data mining and a traditional PCR-based strategy using degenerate primers designed from conserved amino acid sequences of insect capa genes. Our findings demonstrate that ants possess and express a capa gene. As shown by MALDI-TOF mass spectrometry, processed products of the S. invicta capa gene include three CAPA periviscerokinins and low amounts of a pyrokinin which does not have the C-terminal WFGPRLa motif typical of CAPA pyrokinins in other insects. The capa gene was found with two alternative transcripts in the CNS. Within the ventral nerve cord, two capa neurons were immunostained in abdominal neuromeres 2-5, respectively, and projected into ventrally located abdominal perisympathetic organs (PSOs), which are the major hormone release sites of abdominal ganglia. The ventral location of these PSOs is a characteristic feature and was also found in another ant, Atta sexdens. C1 [Choi, Man-Yeon; Meer, Robert K. Vander] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Koehler, Rene; Neupert, Susanne; Predel, Reinhard] Univ Cologne, Bioctr, Inst Zool, D-50931 Cologne, Germany. RP Choi, MY (reprint author), USDA ARS, Hort Crops Res Unit, Corvallis, OR USA. EM mychoi@ars.usda.gov; rpredel@uni-koeln.de RI Predel, Reinhard/O-5243-2015; OI Choi, Man-Yeon/0000-0003-0769-380X FU Deutsche Forschungsgemeinschaft [Predel 595/6-1, Predel 595/6-2, 766/9-1]; Luxemburg Foundation FX The authors acknowledge the financial assistance of the Deutsche Forschungsgemeinschaft (Predel 595/6-1, 2; 766/9-1) and Luxemburg Foundation ( to RK). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 49 TC 5 Z9 5 U1 0 U2 4 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2014 VL 9 IS 4 DI 10.1371/journal.pone.0094274 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE9PC UT WOS:000334339000100 ER PT J AU Holden, AR Koch, JB Griswold, T Erwin, DM Hall, J AF Holden, Anna R. Koch, Jonathan B. Griswold, Terry Erwin, Diane M. Hall, Justin TI Leafcutter Bee Nests and Pupae from the Rancho La Brea Tar Pits of Southern California: Implications for Understanding the Paleoenvironment of the Late Pleistocene SO PLOS ONE LA English DT Article ID MIDDLE EOCENE; HYMENOPTERA-MEGACHILIDAE; APOIDEA; LEAF; COLEOPTERA; FOSSIL; EVOLUTION; BEETLES; CLIMATE; INSECT AB The Rancho La Brea Tar Pits is the world's richest and most important Late Pleistocene fossil locality and best renowned for numerous fossil mammals and birds excavated over the past century. Less researched are insects, even though these specimens frequently serve as the most valuable paleoenvironemental indicators due to their narrow climate restrictions and life cycles. Our goal was to examine fossil material that included insect-plant associations, and thus an even higher potential for significant paleoenviromental data. Micro-CT scans of two exceptionally preserved leafcutter bee nest cells from the Rancho La Brea Tar Pits in Los Angeles, California reveal intact pupae dated between, similar to 23,000-40,000 radiocarbon years BP. Here identified as best matched to Megachile (Litomegachile) gentilis Cresson (Hymenoptera: Megachilidae) based on environmental niche models as well as morphometrics, the nest cells (LACMRLP 388E) document rare preservation and life-stage. The result of complex plant-insect interactions, they offer new insights into the environment of the Late Pleistocene in southern California. The remarkable preservation of the nest cells suggests they were assembled and nested in the ground where they were excavated. The four different types of dicotyledonous leaves used to construct the cells were likely collected in close proximity to the nest and infer a wooded or riparian habitat with sufficient pollen sources for larval provisions. LACMRLP 388E is the first record of fossil Megachile Latreille cells with pupae. Consequently, it provides a pre-modern age location for a Nearctic group, whose phylogenetic relationships and biogeographic history remain poorly understood. Megachile gentilis appears to respond to climate change as it has expanded its distribution across elevation gradients over time as estimated by habitat suitability comparisons between low and high elevations; it currently inhabits mesic habitats which occurred at a lower elevation during the Last Glacial Maximum,21,000 years ago. Nevertheless, the broad ecological niche of M. gentilis appears to have remained stable. C1 [Holden, Anna R.] Nat Hist Museum Los Angeles Cty, Entomol Sect, Los Angeles, CA 90007 USA. [Koch, Jonathan B.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Koch, Jonathan B.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Griswold, Terry] Utah State Univ, USDA ARS, Pollinating Insect Res Unit, Logan, UT 84322 USA. [Erwin, Diane M.] Univ Calif Berkeley, Museum Paleontol, Berkeley, CA 94720 USA. [Hall, Justin] Nat Hist Museum Los Angeles Cty, Dinosaur Inst, Los Angeles, CA USA. RP Holden, AR (reprint author), Nat Hist Museum Los Angeles Cty, Entomol Sect, Los Angeles, CA 90007 USA. EM aholden@nhm.org FU Annie M. Alexander Endowment FX The Annie M. Alexander Endowment provided funding for Diane M. Erwin. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 0 Z9 0 U1 1 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2014 VL 9 IS 4 DI 10.1371/journal.pone.0094724 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE9PC UT WOS:000334339000139 ER PT J AU Lee, DH Cullum, JP Anderson, JL Daugherty, JL Beckett, LM Leskey, TC AF Lee, Doo-Hyung Cullum, John P. Anderson, Jennifer L. Daugherty, Jodi L. Beckett, Lisa M. Leskey, Tracy C. TI Characterization of Overwintering Sites of the Invasive Brown Marmorated Stink Bug in Natural Landscapes Using Human Surveyors and Detector Canines SO PLOS ONE LA English DT Article ID HALYS HEMIPTERA-PENTATOMIDAE; HETEROPTERA; ABILITY; STAL; PEST AB Halyomorpha halys is an invasive species from Asia causing major economic losses in agricultural production in the mid-Atlantic region of the United States. Unlike other crop pests, H. halys is also well-known for nuisance problems in urban, suburban, and rural areas, as massive numbers of adults often invade human-made structures to overwinter inside protected environments. Research efforts have focused on populations in human-made structures while overwintering ecology of H. halys in natural landscapes is virtually unknown. We explored forested landscapes in the mid-Atlantic region to locate and characterize natural overwintering structures used by H. halys. We also evaluated the use of detector canines to locate overwintering H. halys to enhance the accuracy and efficiency of surveys. From these studies, we indentified shared characteristics of overwintering sites used by H. halys in natural landscapes. Overwintering H. halys were recovered from dry crevices in dead, standing trees with thick bark, particularly oak (Quercus spp.)and locust (Robinia spp.); these characteristics were shared by 11.8% of all dead trees in surveyed landscapes. For trees with favorable characteristics, we sampled similar to 20% of the total above-ground tree area and recovered 5.9 adults per tree from the trees with H. halys present. Two detector canines were successfully trained to recognize and detect the odor of adult H. halys yielding >84% accuracy in laboratory and semi-field trials. Detector canines also found overwintering H. halys under field conditions. In particular, overwintering H. halys were recovered only from dead trees that yielded positive indications from the canines and shared key tree characteristics established by human surveyors. The identified characteristics of natural overwintering sites of H. halys will serve as baseline information to establish crop economic risk levels posed by overwintering populations, and accordingly develop sustainable management programs. C1 [Lee, Doo-Hyung; Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Cullum, John P.] Virginia Tech, Dept Entomol, Winchester, VA USA. [Anderson, Jennifer L.; Daugherty, Jodi L.; Beckett, Lisa M.] Anim & Plant Hlth Inspect Serv, USDA, Natl Detector Dog Training Ctr, Newnan, GA USA. RP Lee, DH (reprint author), Gachon Univ, Dept Life Sci, Songnam, Gyeonggi Do, South Korea. EM dl343@cornell.edu FU USDA-NIFA SCRI [2011-51181-30937] FX This work was supported in part by USDA-NIFA SCRI # 2011-51181-30937 award and a specific cooperative agreement with USDA-APHIS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 30 TC 12 Z9 12 U1 6 U2 49 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 9 PY 2014 VL 9 IS 4 AR e91575 DI 10.1371/journal.pone.0091575 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE9PC UT WOS:000334339000010 PM 24717734 ER PT J AU Canaan, A DeFuria, J Perelman, E Schultz, V Seay, M Tuck, D Flavell, RA Snyder, MP Obin, MS Weissman, SM AF Canaan, Allon DeFuria, Jason Perelman, Eddie Schultz, Vincent Seay, Montrell Tuck, David Flavell, Richard A. Snyder, Michael P. Obin, Martin S. Weissman, Sherman M. TI Extended lifespan and reduced adiposity in mice lacking the FAT10 gene SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE longevity; obesity; mammals ID ACTIVATED PROTEIN-KINASE; PROINFLAMMATORY CYTOKINES; CALORIE RESTRICTION; UBIQUITIN FAMILY; INSULIN-RECEPTOR; SKELETAL-MUSCLE; DENDRITIC CELLS; INFLAMMATION; DEGRADATION; LONGEVITY AB The HLA-F adjacent transcript 10 (FAT10) is a member of the ubiquitin-like gene family that alters protein function/stability through covalent ligation. Although FAT10 is induced by inflammatory mediators and implicated in immunity, the physiological functions of FAT10 are poorly defined. We report the discovery that FAT10 regulates lifespan through pleiotropic actions on metabolism and inflammation. Median and overall lifespan are increased 20% in FAT10ko mice, coincident with elevated metabolic rate, preferential use of fat as fuel, and dramatically reduced adiposity. This phenotype is associated with metabolic reprogramming of skeletal muscle (i.e., increased AMP kinase activity, beta-oxidation and -uncoupling, and decreased triglyceride content). Moreover, knockout mice have reduced circulating glucose and insulin levels and enhanced insulin sensitivity in metabolic tissues, consistent with elevated IL-10 in skeletal muscle and serum. These observations suggest novel roles of FAT10 in immune metabolic regulation that impact aging and chronic disease. C1 [Canaan, Allon; Seay, Montrell; Weissman, Sherman M.] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA. [Schultz, Vincent] Yale Univ, Sch Med, Dept Pediat, New Haven, CT 06520 USA. [Tuck, David] Yale Univ, Sch Med, Dept Pathol, New Haven, CT 06520 USA. [Flavell, Richard A.] Yale Univ, Sch Med, Dept Immunobiol, New Haven, CT 06520 USA. [DeFuria, Jason; Obin, Martin S.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Obes & Metab Lab, Boston, MA 02111 USA. [Perelman, Eddie] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel. [Snyder, Michael P.] Stanford Univ, Dept Genet, Stanford, CA 94305 USA. RP Canaan, A (reprint author), Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA. EM allon.canaan@yale.edu FU William Prusoff Foundation; National Institutes of Health [T32DK062032, R01DK074979]; US Department of Agriculture Agricultural Research Service Contract [58-1950-7-70] FX For reading the manuscript and providing very useful remarks, we would like to thank Prof. Diane Krause (Department of Laboratory Medicine, Cell Biology and Pathology), Dr. Stacey N. Brown (Department of Internal Medicine, Section of Endocrinology), and Dr. Victoria E. Clark (Department of Neurosurgery) from Yale School of Medicine. We thank the late Prof. William Prusoff (Department of Pharmacology, Yale School of Medicine) and the William Prusoff Foundation for supporting the studies in this manuscript. Additional funding came from National Institutes of Health Grants T32DK062032 (to J.D.) and R01DK074979 (to M.S.O.) and US Department of Agriculture Agricultural Research Service Contract 58-1950-7-70. NR 62 TC 12 Z9 14 U1 0 U2 10 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 8 PY 2014 VL 111 IS 14 BP 5313 EP 5318 DI 10.1073/pnas.1323426111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE4VU UT WOS:000333985200063 PM 24706839 ER PT J AU Guanter, L Zhang, YG Jung, M Joiner, J Voigt, M Berry, JA Frankenberg, C Huete, AR Zarco-Tejada, P Lee, JE Moran, MS Ponce-Campos, G Beer, C Camps-Valls, G Buchmann, N Gianelle, D Klumpp, K Cescatti, A Baker, JM Griffis, TJ AF Guanter, Luis Zhang, Yongguang Jung, Martin Joiner, Joanna Voigt, Maximilian Berry, Joseph A. Frankenberg, Christian Huete, Alfredo R. Zarco-Tejada, Pablo Lee, Jung-Eun Moran, M. Susan Ponce-Campos, Guillermo Beer, Christian Camps-Valls, Gustavo Buchmann, Nina Gianelle, Damiano Klumpp, Katja Cescatti, Alessandro Baker, John M. Griffis, Timothy J. TI Global and time-resolved monitoring of crop photosynthesis with chlorophyll fluorescence SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE crop productivity; carbon fluxes; Earth observation; carbon modeling; spaceborne spectroscopy ID SUN-INDUCED FLUORESCENCE; GROSS PRIMARY PRODUCTION; CARBON-DIOXIDE; CLIMATE; PRODUCTIVITY; SPACE; GOSAT; HEAT; AGRICULTURE; VARIABILITY AB Photosynthesis is the process by which plants harvest sunlight to produce sugars from carbon dioxide and water. It is the primary source of energy for all life on Earth; hence it is important to understand how this process responds to climate change and human impact. However, model-based estimates of gross primary production (GPP, output from photosynthesis) are highly uncertain, in particular over heavily managed agricultural areas. Recent advances in spectroscopy enable the space-based monitoring of sun-induced chlorophyll fluorescence (SIF) from terrestrial plants. Here we demonstrate that spaceborne SIF retrievals provide a direct measure of the GPP of cropland and grassland ecosystems. Such a strong link with crop photosynthesis is not evident for traditional remotely sensed vegetation indices, nor for more complex carbon cycle models. We use SIF observations to provide a global perspective on agricultural productivity. Our SIF-based crop GPP estimates are 50-75% higher than results from state-of-the-art carbon cycle models over, for example, the US Corn Belt and the Indo-Gangetic Plain, implying that current models severely underestimate the role of management. Our results indicate that SIF data can help us improve our global models for more accurate projections of agricultural productivity and climate impact on crop yields. Extension of our approach to other ecosystems, along with increased observational capabilities for SIF in the near future, holds the prospect of reducing uncertainties in the modeling of the current and future carbon cycle. C1 [Guanter, Luis; Zhang, Yongguang; Voigt, Maximilian] Free Univ Berlin, Inst Space Sci, D-12165 Berlin, Germany. [Jung, Martin] Max Planck Inst Biogeochem, Dept Biogeochem Syst, D-07745 Jena, Germany. [Joiner, Joanna] NASA, Lab Atmospher Chem & Dynam Code 614, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Berry, Joseph A.] Carnegie Inst Sci, Dept Global Ecol, Stanford, CA 94305 USA. [Frankenberg, Christian] CALTECH, Jet Prop Lab, Pasadena, CA 91109 USA. [Huete, Alfredo R.] Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, Sydney, NSW 2007, Australia. [Zarco-Tejada, Pablo] CSIC, Inst Agr Sostenible, Cordoba 14004, Spain. [Lee, Jung-Eun] Brown Univ, Providence, RI 02912 USA. [Moran, M. Susan; Ponce-Campos, Guillermo] ARS, USDA, Tucson, AZ 85719 USA. [Beer, Christian] Stockholm Univ, Dept Appl Environm Sci, S-10691 Stockholm, Sweden. [Beer, Christian] Stockholm Univ, Bolin Ctr Climate Res, S-10691 Stockholm, Sweden. [Camps-Valls, Gustavo] Univ Valencia, Image Proc Lab, Valencia 46980, Spain. [Buchmann, Nina] Eidgenossiche Tech Hsch Zurich, CH-8092 Zurich, Switzerland. [Gianelle, Damiano] Fdn Edmund Mach, Res & Innovat Ctr, Sustainable Agroecosyst & Bioresources Dept, I-38010 San Michele All Adige, Italy. [Klumpp, Katja] INRA, Grassland Ecosyst Res Unit, F-63122 Clermont Ferrand, France. [Cescatti, Alessandro] Commiss European Communities, Joint Res Ctr, Inst Environm & Sustainabil, I-20127 Ispra, Italy. [Baker, John M.] ARS, USDA, St Paul, MN 55108 USA. [Griffis, Timothy J.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. RP Guanter, L (reprint author), Free Univ Berlin, Inst Space Sci, D-12165 Berlin, Germany. EM luis.guanter@wew.fu-berlin.de RI Griffis, Timothy/A-5707-2011; Joiner, Joanna/D-6264-2012; Beer, Christian/D-2296-2013; Zarco-Tejada, Pablo J./A-6874-2012; Guanter, Luis/I-1588-2015; Buchmann, Nina/E-6095-2011; Gianelle, Damiano/G-9437-2011; Huete, Alfredo/C-1294-2008; Frankenberg, Christian/A-2944-2013 OI Zhang, Yongguang/0000-0001-8286-300X; Zarco-Tejada, Pablo J./0000-0003-1433-6165; Guanter, Luis/0000-0002-8389-5764; Gianelle, Damiano/0000-0001-7697-5793; Huete, Alfredo/0000-0003-2809-2376; Frankenberg, Christian/0000-0002-0546-5857 FU German Research Foundation; National Aeronautics and Space Administration (NASA) [NNH10DA001N]; NASA Soil Moisture Active Passive Science Definition Team [08-SMAPSDT08-0042]; W. M. Keck Foundation FX We thank T. Meyers (National Oceanic and Atmospheric Administration Air Resources Laboratory), D. Cook and R. Matamala (Argonne National Laboratory), A. Suyker ( University of Nebraska), C. Bernhofer (Technische Universitat Dresden), Z. Nagy (Szent Istvan University), M. Aubinet (Universite de Liege), W. Kutsch (Johann Heinrich von Thuenen Institut), and K. Schneider (University of Cologne) for kindly providing eddy covariance data. We acknowledge C. Monfreda (Arizona State University), P. H. Verburg (Vrije Universiteit University Amsterdam), and N. Ramankutty (McGill University) for the crop fraction and NPP data sets and/or advice on their use, Eumetsat for the GOME-2 data, the Trendy project for the process-based model runs, and the USDA NASS for their agricultural inventory data. We also thank the two anonymous reviewers and Dr. Asner for their valuable suggestions and comments. MODIS MOD17 GPP data were downloaded from the server of the Numerical Terradynamic Simulation Group at the University of Montana, MODIS MOD13 data were obtained from the MODIS Land Processes Distributed Active Archive Center archive, and MERIS-MTCI from the Infoterra Ltd server. This work used eddy covariance data acquired by AmeriFlux and GHG-Europe. The work by L. G., Y.Z., and M. V. has been funded by the Emmy Noether Programme (GlobFluo project) of the German Research Foundation. J.J. is supported by the National Aeronautics and Space Administration (NASA) Carbon Cycle Science program (NNH10DA001N) and G.P.-C. is supported by NASA Soil Moisture Active Passive Science Definition Team (08-SMAPSDT08-0042). We also thank the W. M. Keck Foundation for funding the New Methods to Measure Photosynthesis from Space workshop held at the Caltech Keck Institute for Space Studies. NR 43 TC 105 Z9 107 U1 19 U2 157 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD APR 8 PY 2014 VL 111 IS 14 BP E1327 EP E1333 DI 10.1073/pnas.1320008111 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE4VU UT WOS:000333985200005 PM 24706867 ER PT J AU Parvizi, P Abdul-Careem, MF Mallick, AI Haq, K Haghighi, HR Orouji, S Heidari, M Behboudi, S Sharif, S AF Parvizi, Payvand Abdul-Careem, Mohamed Faizal Mallick, Amirul Islam Haq, Kamran Haghighi, Hamid R. Orouji, Shahriar Heidari, Mohammad Behboudi, Shahriar Sharif, Shayan TI The effects of administration of ligands for Toll-like receptor 4 and 21 against Marek's disease in chickens SO VACCINE LA English DT Article DE TLR4; TLR21; Cytokines; Marek's disease; Immunity ID CPG OLIGODEOXYNUCLEOTIDES; IMMUNE-SYSTEM; NITRIC-OXIDE; INTERFERON-GAMMA; NEONATAL CHICKS; GENE-EXPRESSION; VIRUS-INFECTION; HOST RESPONSES; B-CELLS; MOTIFS AB Ligands for Toll-like receptors (TLRs) are known to stimulate immune responses, leading to protection against bacterial and viral pathogens. Here, we aimed to examine the effects of various TLR ligands on the development of Marek's disease in chickens. Specific-pathogen free chickens were treated with a series of TLR ligands that interact with TLR3, TLR9 and TLR21. In a pilot study, it was determined that TLR4 and TLR21 ligands are efficacious, in that they could reduce the incidence of Marek's disease tumors in infected birds. Hence, in a subsequent study, chickens were treated with lipopolysaccharide (LPS) as a TLR4 and CpG oligodeoxynucleotides (ODN) as TLR21 agonists before being challenged with the RB1B strain of Marek's disease virus (MDV) via the respiratory route. The results demonstrated that the administration of LPS or CpG ODN, but not PBS or non-CpG ODN, delayed disease onset and reduced MDV genome copy number in the spleens of infected chickens. Taken together, our data demonstrate that TLR4 and 21 agonists modulate anti-virus innate immunity including cytokine responses in MD-infected chicken and this response can only delay, but not inhibit, disease progression. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Parvizi, Payvand; Haghighi, Hamid R.; Orouji, Shahriar; Sharif, Shayan] Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. [Abdul-Careem, Mohamed Faizal] Univ Calgary, Fac Vet Med, Dept Ecosyst & Publ Hlth, Calgary, AB 72N 4N1, Canada. [Mallick, Amirul Islam] Univ Toronto, Sunnybrook Hlth Sci Ctr, Sunnybrook Res Inst, Dept Imaging Res, Toronto, ON M4N 3M5, Canada. [Haq, Kamran] Univ Toronto, Dept Med, Toronto, ON M4N 3M5, Canada. [Heidari, Mohammad] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Behboudi, Shahriar] Pirbright Inst, Compton Lab, Newbury RG20 7NN, Berks, England. RP Sharif, S (reprint author), Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada. EM shayan@uoguelph.ca RI Institute, Pirbright/K-4476-2014 FU Natural Sciences and Engineering Research Council of Canada (NSERC)-CRD Partnerships Program; Poultry Industry Council FX Payvand Parvizi is a recipient of an Alexander Graham Bell Canada Graduate Scholarship (CGS). This study was funded by Natural Sciences and Engineering Research Council of Canada (NSERC)-CRD Partnerships Program, and Poultry Industry Council. We would like to thank Dr. Douglas Hodgins for his help with the statistical analysis of the data. We would also like to acknowledge the staff of the animal isolation facility of University of Guelph. NR 49 TC 5 Z9 5 U1 2 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD APR 7 PY 2014 VL 32 IS 17 BP 1932 EP 1938 DI 10.1016/j.vaccine.2014.01.082 PG 7 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA AH3IK UT WOS:000336016700004 PM 24530927 ER PT J AU Weng, QH Fu, P Gao, F AF Weng, Qihao Fu, Peng Gao, Feng TI Generating daily land surface temperature at Landsat resolution by fusing Landsat and MODIS data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Data fusion; Thermal infrared data; Land surface temperature; Annual temperature cycle; Urban landscape heterogeneity; Thermal imagery sharpening ID URBAN HEAT-ISLAND; WEST-NILE-VIRUS; SATELLITE IMAGERY; TRIANGLE METHOD; ENERGY FLUXES; FOREST COVER; TIME-SERIES; REFLECTANCE; EMISSIVITY; RETRIEVAL AB Land surface temperature (LST) is a crucial parameter in investigating environmental, ecological processes and climate change at various scales, and is also valuable in the studies of evapotranspiration, soil moisture conditions, surface energy balance, and urban heat islands. These studies require thermal infrared (TIR) images at both high temporal and spatial resolution to retrieve LST. However, currently, no single satellite sensors can deliver TIR data at both high temporal and spatial resolution. Thus, various algorithms/models have been developed to enhance the spatial or the temporal resolution of TIR data, but rare of those can enhance both spatial and temporal details. This paper presents a new data fusion algorithm for producing Landsat-like LST data by blending daily MODIS and periodic Landsat TM datasets. The original Spatial and Temporal Adaptive Reflectance Fusion Model (STARFM) was improved and modified for predicting thermal radiance and LST data by considering annual temperature cycle (ATC) and urban thermal landscape heterogeneity. The technique of linear spectral mixture analysis was employed to relate the Landsat radiance with the MODIS one, so that the temporal changes in radiance can be incorporated in the fusion model. This paper details the theoretical basis and the implementation procedures of the proposed data fusion algorithm, Spatio-temporal Adaptive Data Fusion Algorithm for Temperature mapping (SADFAT). A case study was conducted that predicted LSTs of five dates in 2005 from July to October in Los Angeles County, California. The results indicate that the prediction accuracy for the whole study area ranged from 13 K to 2 K Like existing spatio-temporal data fusion models, the SADFAT method has a limitation in predicting LST changes that were not recorded in the MODIS and/or Landsat pixels due to the model assumption. (C) 2014 Elsevier Inc. All rights resereved. C1 [Weng, Qihao; Fu, Peng] Indiana State Univ, Ctr Urban & Environm Change, Dept Earth & Environm Syst, Terre Haute, IN 47809 USA. [Gao, Feng] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Weng, QH (reprint author), Indiana State Univ, Ctr Urban & Environm Change, Dept Earth & Environm Syst, Terre Haute, IN 47809 USA. EM qweng@indstate.edu OI Weng, Qihao/0000-0002-2498-0934 NR 58 TC 47 Z9 50 U1 18 U2 98 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD APR 5 PY 2014 VL 145 BP 55 EP 67 DI 10.1016/j.rse.2014.02.003 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AG0NW UT WOS:000335113200006 ER PT J AU Garabedian, JE McGaughey, RJ Reutebuch, SE Parresol, BR Kilgo, JC Moorman, CE Peterson, MN AF Garabedian, James E. McGaughey, Robert J. Reutebuch, Stephen E. Parresol, Bernard R. Kilgo, John C. Moorman, Christopher E. Peterson, M. Nils TI Quantitative analysis of woodpecker habitat using high-resolution airborne LiDAR estimates of forest structure and composition SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Habitat conservation; Forest structure; Landscape; Prediction confidence interval; Red-cockaded woodpecker; Remote sensing; Savannah River Site; Spatially explicit; Wildlife ID RED-COCKADED WOODPECKER; SMALL-FOOTPRINT; VEGETATION STRUCTURE; SPECIES RICHNESS; SQUIRREL HABITAT; WOODLAND BIRDS; BOREAL FORESTS; HEIGHT; PINE; CAPERCAILLIE AB Light detection and ranging (LiDAR) technology has the potential to radically alter the way researchers and managers collect data on wildlife-habitat relationships. To date, the technology has fostered several novel approaches to characterizing avian habitat, but has been limited by the lack of detailed LiDAR-habitat attributes relevant to species across a continuum of spatial grain sizes and habitat requirements. We demonstrate a novel three-step approach for using LiDAR data to evaluate habitat based on multiple habitat attributes and accounting for their influence at multiple grain sizes using federally endangered red-cockaded woodpecker (RCW; Picoides borealis) foraging habitat data from the Savannah River Site (SRS) in South Carolina, USA First, we used high density LiDAR data (10 returns/m(2)) to predict detailed forest attributes at 20-m resolution across the entire SRS using a complementary application of nonlinear seemingly unrelated regression and multiple linear regression models. Next, we expanded on previous applications of LiDAR by constructing 95% joint prediction confidence intervals to quantify prediction error at various spatial aggregations and habitat thresholds to determine a biologically and statistically meaningful grain size. Finally, we used aggregations of 20-m cells and associated confidence interval boundaries to demonstrate a new approach to produce maps of RCW foraging habitat conditions based on the guidelines described in the species' recovery plan. Predictive power (R-2) of regression models developed to populate raster layers ranged from 034 to 0.81, and prediction error decreased as aggregate size increased, but minimal reductions in prediction error were observed beyond 0.64-ha (4 x 4 20-m cells) aggregates. Mapping habitat quality while accounting for prediction error provided a robust method to determine the potential range of habitat conditions and specific attributes that were limiting in terms of the amount of suitable habitat. The sequential steps of our analytical approach provide a useful framework to extract detailed and reliable habitat attributes for a forest-dwelling habitat specialist, broadening the potential to apply LiDAR in conservation and management of wildlife populations. (C) 2014 Elsevier Inc. All rights reserved. C1 [Garabedian, James E.] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [McGaughey, Robert J.; Reutebuch, Stephen E.] Univ Washington, USDA Forest Serv, Pacific Northwest Stnn, Seattle, WA 98195 USA. [Parresol, Bernard R.] USDA Forest Serv, Southern Res Stn, Asheville, NC 28803 USA. [Kilgo, John C.] USDA Forest Serv, Southern Res Stn, New Ellenton, SC 29809 USA. [Moorman, Christopher E.; Peterson, M. Nils] N Carolina State Univ, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. RP Garabedian, JE (reprint author), N Carolina State Univ, Fisheries Wildlife & Conservat Biol Program, Raleigh, NC 27695 USA. EM jegarabe@ncsu.edu FU U.S. Department of Energy, Savannah River Operations Office through the USDA Forest Service Savannah River [DE-AI09-00SR22188, SRS 10-CS-11083601-003] FX We thank the USDA Forest Service-Savannah River staff for operational support and oversight for contract work involved with the acquisition of LiDAR data used in this study. We also thank J. I. Blake for commenting on previous versions of this manuscript Funding support was provided by U.S. Department of Energy, Savannah River Operations Office through the USDA Forest Service Savannah River (Interagency Agreement DE-AI09-00SR22188) under Cooperative Agreement #SRS 10-CS-11083601-003. We thank A. Poole with the USDA Forest Service-Savannah River for assistance in developing LiDAR raster layers. NR 77 TC 7 Z9 7 U1 6 U2 51 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD APR 5 PY 2014 VL 145 BP 68 EP 80 DI 10.1016/j.rse.2014.01.022 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AG0NW UT WOS:000335113200007 ER PT J AU Roy, DP Wulder, MA Loveland, TR Woodcock, CE Allen, RG Anderson, MC Helder, D Irons, JR Johnson, DM Kennedy, R Scambos, T Schaaf, CB Schott, JR Sheng, Y Vermote, EF Belward, AS Bindschadler, R Cohen, WB Gao, F Hipple, JD Hostert, P Huntington, J Justice, CO Kilic, A Kovalskyy, V Lee, ZP Lymbumer, L Masek, JG McCorkel, J Shuai, Y Trezza, R Vogelmann, J Wynne, RH Zhu, Z AF Roy, D. P. Wulder, M. A. Loveland, T. R. Woodcock, C. E. Allen, R. G. Anderson, M. C. Helder, D. Irons, J. R. Johnson, D. M. Kennedy, R. Scambos, Ta. Schaaf, C. B. Schott, J. R. Sheng, Y. Vermote, E. F. Belward, A. S. Bindschadler, R. Cohen, W. B. Gao, F. Hipple, J. D. Hostert, P. Huntington, J. Justice, C. O. Kilic, A. Kovalskyy, V. Lee, Z. P. Lymbumer, L. Masek, J. G. McCorkel, J. Shuai, Y. Trezza, R. Vogelmann, J. Wynne, R. H. Zhu, Z. TI Landsat-8: Science and product vision for terrestrial global change research SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat 8; OLI; TIRS; Landsat Science Team ID CONTERMINOUS UNITED-STATES; DATA CONTINUITY MISSION; TERM ACQUISITION PLAN; ABSOLUTE RADIOMETRIC CALIBRATION; PLUS ATMOSPHERIC CORRECTION; GREENLAND ICE-SHEET; FOREST COVER CHANGE; LEAF-AREA INDEX; TIME-SERIES; LAND-COVER AB Landsat 8, a NASA and USGS collaboration, acquires global moderate-resolution measurements of the Earth's terrestrial and polar regions in the visible, near-infrared, short wave, and thermal infrared. Landsat 8 extends the remarkable 40 year Landsat record and has enhanced capabilities including new spectral bands in the blue and cirrus cloud-detection portion of the spectrum, two thermal bands, improved sensor signal-to-noise performance and associated improvements in radiometric resolution, and an improved duty cycle that allows collection of a significantly greater number of images per day. This paper introduces the current (2012-2017) Landsat Science Team's efforts to establish an initial understanding of Landsat 8 capabilities and the steps ahead in support of priorities identified by the team. Preliminary evaluation of Landsat 8 capabilities and identification of new science and applications opportunities are described with respect to calibration and radiometric characterization; surface reflectance; surface albedo; surface temperature, evapotranspiration and drought; agriculture; land cover, condition, disturbance and change; fresh and coastal water; and snow and ice. Insights into the development of derived 'higher-lever Landsat products are provided in recognition of the growing need for consistently processed, moderate spatial resolution, large area, long-term terrestrial data records for resource management and for climate and global change studies. The paper concludes with future prospects, emphasizing the opportunities for land imaging constellations by combining Landsat data with data collected from other international sensing systems, and consideration of successor Landsat mission requirements. (c) 2014 The Authors. Published by Elsevier Inc. C1 [Roy, D. P.; Kovalskyy, V.] S Dakota State Univ, Geog Informat Sci Ctr Excellence, Brookings, SD 57007 USA. [Wulder, M. A.] Nat Resources Canada, Pacific Forestry Ctr, Canadian Forest Serv, Victoria, BC V8Z 1M5, Canada. [Loveland, T. R.; Vogelmann, J.] US Geol Survey, EROS, Sioux Falls, SD 57198 USA. [Woodcock, C. E.; Kennedy, R.; Zhu, Z.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Allen, R. G.; Trezza, R.] Univ Idaho, Res & Extens Ctr, Kimberly, ID 83341 USA. [Anderson, M. C.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Helder, D.] S Dakota State Univ, Coll Engn, Brookings, SD 57007 USA. [Irons, J. R.] NASA, Goddard Space Flight Ctr, Atmospheres Lab, Greenbelt, MD 20771 USA. [Johnson, D. M.] USDA, Natl Agr Stat Serv, Fairfax, VA 22030 USA. [Scambos, Ta.] Univ Colorado, Natl Snow & Ice Data Ctr, Boulder, CO 80303 USA. [Schaaf, C. B.; Lee, Z. P.] Univ Massachusetts, Sch Environm, Boston, MA 02125 USA. [Schott, J. R.] Rochester Inst Technol, Chester F Carlson Ctr Imaging Sci, Rochester, NY 14623 USA. [Sheng, Y.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90095 USA. [Vermote, E. F.] NASA, Goddard Space Flight Ctr, Terr Informat Syst Lab, Greenbelt, MD 20771 USA. [Belward, A. S.] European Commiss, Joint Res Ctr, Inst Environm & Sustainabil, I-20133 Milan, Italy. [Bindschadler, R.] NASA, Goddard Space Flight Ctr, Hydrospher & Biospher Sci Lab, Greenbelt, MD 20771 USA. [Cohen, W. B.] USDA Forest Serv, Corvallis, OR 97331 USA. [Gao, F.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Hipple, J. D.] USDA, Risk Management Agcy, Washington, DC 20250 USA. [Hostert, P.] Humboldt Univ, Dept Geog, D-10099 Berlin, Germany. [Huntington, J.] Desert Res Inst, Reno, NV 89501 USA. [Justice, C. O.] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. [Kilic, A.] Univ Nebraska, Sch Nat Resources, Dept Civil Engn, Lincoln, NE 68516 USA. [Lymbumer, L.] Geosci Australia, Canberra, ACT 2601, Australia. [Masek, J. G.; McCorkel, J.] NASA, Goddard Space Flight Ctr, Biospher Sci Lab, Greenbelt, MD 20771 USA. [Shuai, Y.] NASAs, Goddard Space Flight Ctr, ERT Inc, Biospher Sci Lab, Greenbelt, MD 20771 USA. [Wynne, R. H.] Virginia Tech, Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. RP Roy, DP (reprint author), S Dakota State Univ, Geog Informat Sci Ctr Excellence, Brookings, SD 57007 USA. RI Masek, Jeffrey/D-7673-2012; Anderson, Martha/C-1720-2015; McCorkel, Joel/D-4454-2012; Zhu, Zhe/K-3518-2015; Wulder, Michael/J-5597-2016; OI Anderson, Martha/0000-0003-0748-5525; McCorkel, Joel/0000-0003-2853-2036; Zhu, Zhe/0000-0001-8283-6407; Wulder, Michael/0000-0002-6942-1896; Vogelmann, James/0000-0002-0804-5823 FU USGS-NASA Landsat Science Team FX We are grateful to the NASA-USGS-industry Landsat Data Continuity Mission (LDCM) development team for their efforts to meet an aggressive launch schedule, and we thank the USGS Climate and Land Use Change Mission's Land Remote Sensing Program and the Earth Resources Observation and Science (EROS) Center for co-sponsoring and funding the USGS-NASA Landsat Science Team. NR 194 TC 243 Z9 253 U1 36 U2 223 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD APR 5 PY 2014 VL 145 BP 154 EP 172 DI 10.1016/j.rse.2014.02.001 PG 19 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AG0NW UT WOS:000335113200014 ER PT J AU Xu, JM Jiang, JC Hse, CY Shupe, TF AF Xu, Junming Jiang, Jianchun Hse, Chung-Yun Shupe, Todd F. TI Preparation of Polyurethane Foams Using Fractionated Products in Liquefied Wood SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE biomaterials; biopolymers and renewable polymers; polyurethanes ID LIGNOCELLULOSIC MATERIALS; LIQUEFACTION; BIOPOLYOLS; GLYCEROL; POLYMERS; POLYOLS; STRAW AB Liquefaction of sawdust was studied using glycerol and methanol as mix solvents. A new bio-polyol product consisting of high purity multi-hydroxy compounds was obtained by precipitation of the hydrophobic organics from the liquefied product in an aqueous solution. As identified by GC-MS, the dominate components in bio-polyol were glycerol, glycerol derivatives, and multiple types of sugar derivatives. By using the mass ratio of m (sawdust) : m (glycerol)=1 : 1, the total content of multi-hydroxy compounds reached 90.84%. The hydroxyl number of the bio-polyol was 1287 mgKOH/g with a rotational viscosity of 1270 cP. Preparation of polyurethane foams using bio-polyol and isocyanate was also studied. Water was used as an environmental friendly blowing agent. The factors that influence the cell structure of foams (i.e., catalyst, dosage of blowing agent, and mass ratio of bio-polyol to PEG-400 were studied. The compressive strength of the synthesized foam was 150 Kpa, which met the requirement of Chinese specification for rigid foams. The synthesized foams were characterized by FTIR, SEM, and TG. (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40096. C1 [Xu, Junming; Jiang, Jianchun] CAF, Inst Chem Ind Forestry Prod, Nanjing 210042, Jiangsu, Peoples R China. [Xu, Junming; Jiang, Jianchun] SFA, Key & Lab Forest Chem Engn, Natl Engn Lab Biomass Chem Utilizat, Lab Biomass Energy & Mat, Nanjing 210042, Jiangsu, Peoples R China. [Hse, Chung-Yun] USDA Forest Serv, So Forest Expt Stn, Pineville, LA 71360 USA. [Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. RP Xu, JM (reprint author), CAF, Inst Chem Ind Forestry Prod, Nanjing 210042, Jiangsu, Peoples R China. EM lang811023@163.com FU Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals [JSBGFC12001] FX The authors would like to thank the "Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals (JSBGFC12001) for financial support during this investigation. NR 19 TC 6 Z9 6 U1 4 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD APR 5 PY 2014 VL 131 IS 7 AR 40096 DI 10.1002/app.40096 PG 7 WC Polymer Science SC Polymer Science GA AA3PF UT WOS:000331004100063 ER PT J AU Jensen, PJ Fazio, G Altman, N Praul, C McNellis, TW AF Jensen, Philip J. Fazio, Gennaro Altman, Naomi Praul, Craig McNellis, Timothy W. TI Mapping in an apple (Malus x domestica) F-1 segregating population based on physical clustering of differentially expressed genes SO BMC GENOMICS LA English DT Article DE Powdery mildew disease; Woolly apple aphid; Gene expression marker; DNA microarray; Genetic marker development; Quantitative PCR ID RESISTANCE; GENOME; TRANSCRIPTOMICS; PATTERNS; TOOL AB Background: Apple tree breeding is slow and difficult due to long generation times, self-incompatibility, and complex genetics. The identification of molecular markers linked to traits of interest is a way to expedite the breeding process. In the present study, we aimed to identify genes whose steady-state transcript abundance was associated with inheritance of specific traits segregating in an apple (Malus x domestica) rootstock F-1 breeding population, including resistance to powdery mildew (Podosphaera leucotricha) disease and woolly apple aphid (Eriosoma lanigerum). Results: Transcription profiling was performed for 48 individual F-1 apple trees from a cross of two highly heterozygous parents, using RNA isolated from healthy, actively-growing shoot tips and a custom apple DNA oligonucleotide microarray representing 26,000 unique transcripts. Genome-wide expression profiles were not clear indicators of powdery mildew or woolly apple aphid resistance phenotype. However, standard differential gene expression analysis between phenotypic groups of trees revealed relatively small sets of genes with trait-associated expression levels. For example, thirty genes were identified that were differentially expressed between trees resistant and susceptible to powdery mildew. Interestingly, the genes encoding twenty-four of these transcripts were physically clustered on chromosome 12. Similarly, seven genes were identified that were differentially expressed between trees resistant and susceptible to woolly apple aphid, and the genes encoding five of these transcripts were also clustered, this time on chromosome 17. In each case, the gene clusters were in the vicinity of previously identified major quantitative trait loci for the corresponding trait. Similar results were obtained for a series of molecular traits. Several of the differentially expressed genes were used to develop DNA polymorphism markers linked to powdery mildew disease and woolly apple aphid resistance. Conclusions: Gene expression profiling and trait-associated transcript analysis using an apple F-1 population readily identified genes physically linked to powdery mildew disease resistance and woolly apple aphid resistance loci. This result was especially useful in apple, where extreme levels of heterozygosity make the development of reliable DNA markers quite difficult. The results suggest that this approach could prove effective in crops with complicated genetics, or for which few genomic information resources are available. C1 [Jensen, Philip J.; McNellis, Timothy W.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Fazio, Gennaro] ARS, USDA, Plant Genet Resources Unit, Geneva, NY 14456 USA. [Altman, Naomi] Penn State Univ, Dept Stat, University Pk, PA 16802 USA. [Praul, Craig] Penn State Univ, Huck Inst Life Sci, Genom Core Facil, University Pk, PA 16802 USA. RP McNellis, TW (reprint author), Penn State Univ, Dept Plant Pathol & Environm Microbiol, 211 Buckhout Lab, University Pk, PA 16802 USA. EM twm4@psu.edu FU National Science Foundation [DBI-0420394] FX This work was supported by National Science Foundation Plant Genome Research Program Grant DBI-0420394 to T.W.M., Robert M. Crassweller, Siela N. Maximova, and James W. Travis. NR 22 TC 6 Z9 6 U1 0 U2 29 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD APR 4 PY 2014 VL 15 AR 261 DI 10.1186/1471-2164-15-261 PG 11 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AF8HM UT WOS:000334956400001 PM 24708064 ER PT J AU Passler, T Riddell, KP Edmondson, MA Chamorro, MF Neill, JD Brodersen, BW Walz, HL Galik, PK Zhang, YJ Walz, PH AF Passler, Thomas Riddell, Kay P. Edmondson, Misty A. Chamorro, Manuel F. Neill, John D. Brodersen, Bruce W. Walz, Heather L. Galik, Patricia K. Zhang, Yijing Walz, Paul H. TI Experimental infection of pregnant goats with bovine viral diarrhea virus (BVDV) 1 or 2 SO VETERINARY RESEARCH LA English DT Article ID WHITE-TAILED DEER; ALPACAS LAMA-PACOS; PERSISTENT INFECTION; MONOCLONAL-ANTIBODIES; TRANSPLACENTAL INFECTION; PESTIVIRUS INFECTIONS; FETAL INFECTION; BORDER DISEASE; VICUGNA-PACOS; CATTLE AB Infections with bovine viral diarrhea virus (BVDV) of the genus pestivirus, family Flaviviridae, are not limited to cattle but occur in various artiodactyls. Persistently infected (PI) cattle are the main source of BVDV. Persistent infections also occur in heterologous hosts such as sheep and deer. BVDV infections of goats commonly result in reproductive disease, but viable PI goats are rare. Using 2 BVDV isolates, previously demonstrated to cause PI cattle and white-tailed deer, this study evaluated the outcome of experimental infection of pregnant goats. Pregnant goats (5 goats/group) were intranasally inoculated with BVDV 1b AU526 (group 1) or BVDV 2 PA131 (group 2) at approximately 25-35 days of gestation. The outcome of infection varied considerably between groups. In group 1, only 3 does became viremic, and 1 doe gave birth to a stillborn fetus and a viable PI kid, which appeared healthy and shed BVDV continuously. In group 2, all does became viremic, 4/5 does aborted, and 1 doe gave birth to a non-viable PI kid. Immunohistochemistry demonstrated BVDV antigen in tissues of evaluated fetuses, with similar distribution but reduced intensity as compared to cattle. The genetic sequence of inoculated viruses was compared to those from PI kids and their dam. Most nucleotide changes in group 1 were present during the dam's acute infection. In group 2, a similar number of mutations resulted from fetal infection as from maternal acute infection. Results demonstrated that BVDV may cause reproductive disease but may also be maintained in goats. C1 [Passler, Thomas; Riddell, Kay P.; Edmondson, Misty A.; Chamorro, Manuel F.; Walz, Heather L.; Galik, Patricia K.; Zhang, Yijing; Walz, Paul H.] Auburn Univ, Coll Vet Med, Dept Clin Sci, Auburn, AL 36849 USA. [Passler, Thomas; Riddell, Kay P.; Edmondson, Misty A.; Chamorro, Manuel F.; Walz, Heather L.; Galik, Patricia K.; Zhang, Yijing; Walz, Paul H.] Auburn Univ, Coll Vet Med, Dept Pathobiol, Auburn, AL 36849 USA. [Neill, John D.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Brodersen, Bruce W.] Univ Nebraska, Sch Vet Med & Biomed Sci, Lincoln, NE 68508 USA. RP Passler, T (reprint author), Auburn Univ, Coll Vet Med, Dept Clin Sci, Auburn, AL 36849 USA. EM passlth@auburn.edu FU Animal Health Research, Auburn University FX This study was funded by Animal Health Research, Auburn University. The authors acknowledge the technical staff of the BVDV laboratories at Auburn University, especially Ms. Terri Hathcock. NR 70 TC 1 Z9 2 U1 3 U2 17 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD APR 4 PY 2014 VL 45 AR 38 DI 10.1186/1297-9716-45-38 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA AF3ML UT WOS:000334616300002 PM 24708266 ER PT J AU Dunlap, CA Behle, RW Jackson, MA AF Dunlap, Christopher A. Behle, Robert W. Jackson, Mark A. TI Compatibility of OMRI-certified surfactants with three entomopathogenic fungi SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE entomopathogen; foam; conidia; blastospore; surfactant; formulation; organic ID ORGANOSILICONE SURFACTANT; PAECILOMYCES-FUMOSOROSEUS; NONIONIC SURFACTANT; SILWET L-77; TOXICITY; HEMIPTERA; SAPONINS; EFFICACY; TENSION; BLASTOSPORES AB The Organic Materials Review Institute (OMRI) is a non-profit organisation providing an independent review of products intended for use in organic production systems to certify compliance with US national organic standards. Since all adjuvants to be used in organic agriculture production are required to meet these standards, OMRI's certified list of products is a convenient starting point when developing organic pest control formulations. In the current study, six OMRI-certified surfactants are tested for their compatibility with three common entomopathogens: Beauveria bassiana, Metarhizium brunneum and Isaria fumosorosea. The fungi were evaluated in two common propagule forms, solid-state produced conidia and liquid-media produced blastospores. The results show that most of the surfactants are compatible with the fungi at a high surfactant concentration (2% w/v). In general, the conidia showed a higher susceptibility (greater reduction in spore germination) to the surfactants than the blastospores under these conditions. In addition, the surface tension and foaming properties of the surfactants were determined. C1 [Dunlap, Christopher A.; Behle, Robert W.; Jackson, Mark A.] ARS, USDA, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Dunlap, CA (reprint author), ARS, USDA, Crop Bioprotect Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM christopher.dunlap@ars.usda.gov NR 43 TC 0 Z9 0 U1 2 U2 14 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD APR 3 PY 2014 VL 24 IS 4 BP 436 EP 447 DI 10.1080/09583157.2013.870532 PG 12 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AE4EK UT WOS:000333934400006 ER PT J AU Walsh, GC Maestro, M Sosa, A Tipping, PW AF Cabrera Walsh, Guillermo Maestro, Mariano Sosa, Alejandro Tipping, Philip W. TI Specificity of Lepidelphax pistiae (Hemiptera: Delphacidae) to Pistia stratiotes (Araceae) SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE water lettuce; no-choice tests; aquatic weeds ID WEEVIL AB The delphacid Lepidelphax pistiae was found on water lettuce in Argentina. Nymphs and adults did not survive past seven days on 29 species of test plants, and no progeny were produced. In contrast, survival and reproduction were high on water lettuce. Results suggest that L. pistiae is monospecific to water lettuce. C1 [Cabrera Walsh, Guillermo; Maestro, Mariano; Sosa, Alejandro] Fdn Estudio Especies Invas FUEDEI, Buenos Aires, DF, Argentina. [Tipping, Philip W.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL USA. RP Walsh, GC (reprint author), Fdn Estudio Especies Invas FUEDEI, Buenos Aires, DF, Argentina. EM gcabrera@fuedei.org NR 7 TC 2 Z9 2 U1 2 U2 5 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PD APR 3 PY 2014 VL 24 IS 4 BP 485 EP 488 DI 10.1080/09583157.2013.867012 PG 4 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AE4EK UT WOS:000333934400010 ER PT J AU Lizotte, RE Locke, MA Testa, S AF Lizotte, Richard E., Jr. Locke, Martin A. Testa, Sam, III TI Influence of varying nutrient and pesticide mixtures on abatement efficiency using a vegetated free water surface constructed wetland mesocosm SO CHEMISTRY AND ECOLOGY LA English DT Article DE atrazine; permethrin; best management practices; phosphorus; mitigation; nitrogen; S-metolachlor ID NONPOINT-SOURCE POLLUTION; AGRICULTURAL RUNOFF; ARTIFICIAL WETLAND; SOUTH-AFRICA; MITIGATION; ATRAZINE; REMOVAL; PHOSPHORUS; SEDIMENT; METOLACHLOR AB The nutrient and pesticide abatement efficiency of varying mixtures was examined in a vegetated free water surface constructed wetland. Three different agricultural chemical pollutant mixture conditions were assessed: nutrients only (N and P); pesticides only (atrazine, S-metolachlor and permethrin); and a mixture of nutrients and pesticides. With nutrients only, 672h nutrient mitigation of 77-91% total phosphorous (TP) and 74-98% total nitrogen (TN) was associated with distance from the injection point and rainfall, whereas with nutrient and pesticide mixtures, 672h nutrient mitigation of 11-71% TP and 84-98% TN were associated with distance and time. With pesticides only, 672h pesticide mitigation of 50-99% was associated with distance and time, whereas with nutrients and pesticide mixtures, 672h pesticide mitigation of 48-99% was associated primarily with distance. Dissipation half-lives were 2-10 times greater for P and 1.5-5 times greater for N when pesticides were present. Pesticide dissipation half-lives showed no clear differences with or without nutrients. While vegetated free water surface constructed wetlands can be effective best management practice tools to trap and abate agricultural run-off during rainfall events, efficiencies can be affected by different types of complex pollutant mixtures and wetland design and implementation should accommodate varying efficiencies. C1 [Lizotte, Richard E., Jr.; Locke, Martin A.; Testa, Sam, III] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Lizotte, RE (reprint author), ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. EM richard.lizotte@ars.usda.gov NR 51 TC 1 Z9 1 U1 4 U2 39 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0275-7540 EI 1029-0370 J9 CHEM ECOL JI Chem. Ecol. PD APR 3 PY 2014 VL 30 IS 3 BP 280 EP 294 DI 10.1080/02757540.2013.861823 PG 15 WC Biochemistry & Molecular Biology; Ecology; Environmental Sciences SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology GA AE5YF UT WOS:000334064500009 ER PT J AU Williams, MS Ebel, ED AF Williams, Michael S. Ebel, Eric D. TI Estimating the correlation between concentrations of two species of bacteria with censored microbial testing data SO INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY LA English DT Article DE Campylobacter; Salmonella; Maximum likelihood estimation; Risk assessment ID RISK-ASSESSMENT; LOGNORMAL-DISTRIBUTION; POULTRY; DISTRIBUTIONS; SALMONELLA; BEEF AB Indicator organisms, such as generic Escherichia coli. (GEC) and coliforms, can be used to measure changes in microbial contamination during the production of food products. Large and consistent reductions in the concentration of these organisms demonstrates an effective and well-controlled production process. Nevertheless, it is unclear to what degree concentrations of indicator organisms are related to pathogenic organisms such as Campylobacter and Salmonella on a sample-by-sample basis. If a strong correlation exists between the concentrations of different organisms, then the monitoring of indicator organisms would be a cost-effective surrogate for the measurement of pathogenic organisms. Calculating the correlation between the concentrations of an indicator and pathogenic organism is complicated because microbial testing datasets typically contain a large proportion of censored observations (i.e., samples where the true concentration is not observable, with nondetects and samples that are only screen-test positive being examples). This study proposes a maximum likelihood estimator that can be used to estimate the correlation between the concentrations of indicator and pathogenic organisms. An example based on broiler chicken rinse samples demonstrates modest, but significant positive correlations between the concentration of the indicator organism GEC when compared to the concentration of both Campylobacter and Salmonella. A weak positive correlation was also observed between concentrations of Campylobacter and Salmonella, but it was not statistically significant. Published by Elsevier B.V. C1 [Williams, Michael S.; Ebel, Eric D.] US Food Safety & Inspect Serv, Off Publ Hlth Sci, USDA, Ft Collins, CO 80526 USA. RP Williams, MS (reprint author), 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM mike.williams@fsis.usda.gov NR 28 TC 6 Z9 6 U1 0 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1605 EI 1879-3460 J9 INT J FOOD MICROBIOL JI Int. J. Food Microbiol. PD APR 3 PY 2014 VL 175 BP 1 EP 5 DI 10.1016/j.ijfoodmicro.2014.01.007 PG 5 WC Food Science & Technology; Microbiology SC Food Science & Technology; Microbiology GA AE3DT UT WOS:000333857900001 PM 24491921 ER PT J AU Vasconcelos, MW Clemente, TE Grusak, MA AF Vasconcelos, Marta W. Clemente, Thomas E. Grusak, Michael A. TI Evaluation of constitutive iron reductase (AtFRO2) expression on mineral accumulation and distribution in soybean (Glycinemax.L) SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE FRO2; iron; mineral nutrition; soybean; transgenic ID FERRIC-CHELATE REDUCTASE; METAL-NICOTIANAMINE TRANSPORTER; LONG-DISTANCE TRANSPORT; PISUM-SATIVUM-L; ARABIDOPSIS-THALIANA; DEVELOPING SEEDS; MEDICAGO-TRUNCATULA; DEFICIENCY RESPONSE; FE DEFICIENCY; LIFE-CYCLE AB Iron is an important micronutrient in human and plant nutrition. Adequate iron nutrition during crop production is central for assuring appropriate iron concentrations in the harvestable organs, for human food or animal feed. The whole-plant movement of iron involves several processes, including the reduction of ferric to ferrous iron at several locations throughout the plant, prior to transmembrane trafficking of ferrous iron. In this study, soybean plants that constitutively expressed the AtFRO2 iron reductase gene were analyzed for leaf iron reductase activity, as well as the effect of this transgene's expression on root, leaf, pod wall, and seed mineral concentrations. High Fe supply, in combination with the constitutive expression of AtFRO2, resulted in significantly higher concentrations of different minerals in roots (K, P, Zn, Ca, Ni, Mg, and Mo), pod walls (Fe, K, P, Cu, and Ni), leaves (Fe, P, Cu, Ca, Ni, and Mg) and seeds (Fe, Zn, Cu, and Ni). Leaf and pod wall iron concentrations increased as much as 500% in transgenic plants, while seed iron concentrations only increased by 10%, suggesting that factors other than leaf and pod wall reductase activity were limiting the translocation of iron to seeds. Protoplasts isolated from transgenic leaves had three-fold higher reductase activity than controls. Expression levels of the iron storage protein, ferritin, were higher in the transgenic leaves than in wild-type, suggesting that the excess iron may be stored as ferritin in the leaves and therefore unavailable for phloem loading and delivery to the seeds. Also, citrate and malate levels in the roots and leaves of transgenic plants were significantly higher than in wild-type, suggesting that organic acid production could be related to the increased accumulation of minerals in roots, leaves, and pod walls, but not in the seeds. All together, these results suggest a more ubiquitous role for the iron reductase in whole-plant mineral accumulation and distribution. C1 [Vasconcelos, Marta W.] Univ Catolica Portuguesa, Ctr Reg Porto, Escola Super Biotecnol, Ctr Biotecnol & Quim Fina, P-4200072 Oporto, Portugal. [Vasconcelos, Marta W.; Grusak, Michael A.] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Clemente, Thomas E.] Univ Nebraska, Plant Sci Initiat, Ctr Biotechnol, Lincoln, NE USA. RP Vasconcelos, MW (reprint author), Univ Catolica Portuguesa, Ctr Reg Porto, Escola Super Biotecnol, Ctr Biotecnol & Quim Fina, Rua Dr Antonio Bernardino de Almeida, P-4200072 Oporto, Portugal. EM mvasconcelos@porto.ucp.pt FU USDA-ARS [58-6250-0-008]; FCT; FEDER [PTDC/AGR-GPL/118772/2010] FX This work was supported in part by funds from USDA-ARS under Agreement number 58-6250-0-008 to MAG. The contents of this publication do not necessarily reflect the views or policies of the US Department of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. This work was also financially supported by FCT and FEDER, through the project ref. PTDC/AGR-GPL/118772/2010. We are very thankful to Susana Carvalho for a critical review of the manuscript. NR 62 TC 5 Z9 5 U1 2 U2 30 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD APR 3 PY 2014 VL 5 AR 112 DI 10.3389/fpls.2014.00112 PG 12 WC Plant Sciences SC Plant Sciences GA AE0PN UT WOS:000333668400001 PM 24765096 ER PT J AU Brandon, DL Korn, AM Yang, LL AF Brandon, David L. Korn, Anna M. Yang, Lily L. TI Immunosorbent analysis of ricin contamination in milk using colorimetric, chemiluminescent and electrochemiluminescent detection SO FOOD AND AGRICULTURAL IMMUNOLOGY LA English DT Article DE ricin; Ricinus communis agglutinin; castor; monoclonal antibody ID IN-GROUND BEEF; BIOLOGICAL-ACTIVITY; COMMUNIS AGGLUTININ; ASSAY; ANTIBODIES; ELISA; TOXICITY; SEQUENCE; APTAMER; EGG AB Analytical methodology to detect active ricin in food matrices is important because of the potential use of foodborne ricin as a terrorist weapon. Monoclonal antibodies (mAbs) that bind ricin were used as both capture and detection ligands in sandwich enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescent (ECL) immunosorbent assays. ELISA employed two types of substrate for colorimetric or chemiluminescent detection. Although both fat content and protein content of samples influenced the recovery of ricin, the lower limit of detection (LOD) in ELISA and ECL systems permitted detection of 0.1 ng/mL for milk samples containing 0-4% fat. The assay systems detect pure ricin- or crude ricin-containing castor extract, but do not significantly respond to isolated ricin chains, heat-denatured ricin or the related agglutinin, Ricinus communis agglutinin 1 (RCA-1). Using the standard 96-well-plate formats, the assays detect less than 0.01% of an adult human lethal dose in a typical serving of milk. C1 [Brandon, David L.; Korn, Anna M.; Yang, Lily L.] ARS, Foodborne Contaminants Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Brandon, DL (reprint author), ARS, Foodborne Contaminants Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM david.brandon@ars.usda.gov NR 34 TC 6 Z9 6 U1 4 U2 36 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0954-0105 EI 1465-3443 J9 FOOD AGR IMMUNOL JI Food Agric. Immunol. PD APR 3 PY 2014 VL 25 IS 2 BP 160 EP 172 DI 10.1080/09540105.2012.753515 PG 13 WC Chemistry, Applied; Food Science & Technology; Immunology; Toxicology SC Chemistry; Food Science & Technology; Immunology; Toxicology GA AA2NP UT WOS:000330931600002 ER PT J AU Li, HX Shang, HQ Shu, DM Zhang, HM Ji, J Sun, BL Li, HM Xie, QM AF Li, Hongxin Shang, Huiqing Shu, Dingming Zhang, Huanmin Ji, Jun Sun, Baoli Li, Hongmei Xie, Qingmei TI gga-miR-375 Plays a Key Role in Tumorigenesis Post Subgroup J Avian Leukosis Virus Infection SO PLOS ONE LA English DT Article ID ORGAN SIZE CONTROL; HUMAN CYCLIN-E; CELL-DEATH; MYELOID LEUKOSIS; HPRS-103 STRAIN; HIPPO PATHWAY; HEPATOCELLULAR-CARCINOMA; TUMOR SUPPRESSION; BREAST-CANCER; LIVER-CANCER AB Avian leukosis is a neoplastic disease caused in part by subgroup J avian leukosis virus J (ALV-J). Micro ribonucleic acids (miRNAs) play pivotal oncogenic and tumour-suppressor roles in tumour development and progression. However, little is known about the potential role of miRNAs in avian leukosis tumours. We have found a novel tumour-suppressor miRNA, gga-miR-375, associated with avian leukosis tumorigenesis by miRNA microarray in a previous report. We have also previously studied the biological function of gga-miR-375; Overexpression of gga-miR-375 significantly inhibited DF-1 cell proliferation, and significantly reduced the expression of yes-associated protein 1 (YAP1) by repressing the activity of a luciferase reporter carrying the 3'-untranslated region of YAP1. This indicates that gga-miR-375 is frequently downregulated in avian leukosis by inhibiting cell proliferation through YAP1 oncogene targeting. Overexpression of gga-miR-375 markedly promoted serum starvation induced apoptosis, and there may be the reason why the tumour cycle is so long in the infected chickens. In vivo assays, gga-miR-375 was significantly downregulated in chicken livers 20 days after infection with ALV-J, and YAP1 was significantly upregulated 20 days after ALV-J infection (P<0.05). We also found that expression of cyclin E, an important regulator of cell cycle progression, was significantly upregulated (P<0.05). Drosophila inhibitor of apoptosis protein 1 (DIAP1), which is related to caspase-dependent apoptosis, was also significantly upregulated after infection. Our data suggests that gga-miR-375 may function as a tumour suppressor thereby regulating cancer cell proliferation and it plays a key role in avian leukosis tumorigenesis. C1 [Li, Hongxin; Shang, Huiqing; Ji, Jun; Sun, Baoli; Li, Hongmei; Xie, Qingmei] South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China. [Li, Hongxin; Sun, Baoli; Li, Hongmei; Xie, Qingmei] Minist Agr, Key Lab Chicken Genet Breeding & Reprod, Guangzhou, Guangdong, Peoples R China. [Shu, Dingming] State Key Lab Livestock & Poultry Breeding, Guangzhou, Guangdong, Peoples R China. [Zhang, Huanmin] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. RP Xie, QM (reprint author), South China Agr Univ, Coll Anim Sci, Guangzhou, Guangdong, Peoples R China. EM qmx@scau.edu.cn FU National Natural Science Foundation of China [31072152]; State Key Laboratory of Biocontrol [SKLBC2010K07] FX This work was supported by grants from the National Natural Science Foundation of China (31072152) and the Open Project of the State Key Laboratory of Biocontrol (SKLBC2010K07). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 76 TC 7 Z9 7 U1 2 U2 7 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 2 PY 2014 VL 9 IS 4 AR e90878 DI 10.1371/journal.pone.0090878 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE6LC UT WOS:000334103000010 PM 24694742 ER PT J AU Zhu, XB Richael, C Chamberlain, P Busse, JS Bussan, AJ Jiang, JM Bethke, PC AF Zhu, Xiaobiao Richael, Craig Chamberlain, Patrick Busse, James S. Bussan, Alvin J. Jiang, Jiming Bethke, Paul C. TI Vacuolar Invertase Gene Silencing in Potato (Solanum tuberosum L.) Improves Processing Quality by Decreasing the Frequency of Sugar-End Defects SO PLOS ONE LA English DT Article ID RUSSET-BURBANK POTATOES; FREE AMINO-ACIDS; ACRYLAMIDE FORMATION; MAILLARD REACTION; REDUCING SUGAR; BASAL PORTIONS; STARCH CONTENT; DNA VARIATION; FRENCH FRIES; WATER-STRESS AB Sugar-end defect is a tuber quality disorder and persistent problem for the French fry processing industry that causes unacceptable darkening of one end of French fries. This defect appears when environmental stress during tuber growth increases post-harvest vacuolar acid invertase activity at one end of the tuber. Reducing sugars produced by invertase form dark-colored Maillard reaction products during frying. Acrylamide is another Maillard reaction product formed from reducing sugars and acrylamide consumption has raised health concerns worldwide. Vacuolar invertase gene (VInv) expression was suppressed in cultivars Russet Burbank and Ranger Russet using RNA interference to determine if this approach could control sugar-end defect formation. Acid invertase activity and reducing sugar content decreased at both ends of tubers. Sugar-end defects and acrylamide in fried potato strips were strongly reduced in multiple transgenic potato lines. Thus vacuolar invertase silencing can minimize a long-standing French fry quality problem while providing consumers with attractive products that reduce health concerns related to dietary acrylamide. C1 [Zhu, Xiaobiao; Chamberlain, Patrick; Bussan, Alvin J.; Jiang, Jiming; Bethke, Paul C.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Richael, Craig] JR Simplot Co, Simplot Plant Sci, Boise, ID 83707 USA. [Busse, James S.; Bethke, Paul C.] USDA, Vegetable Crops Res Unit, Madison, WI USA. RP Richael, C (reprint author), JR Simplot Co, Simplot Plant Sci, Boise, ID 83707 USA. EM Craig.Richael@simplot.com; jjiang1@wisc.edu; paul.bethke@ars.usda.gov FU WI DATCP Specialty Crop Research Initiative Block Grant Program; USDA NIFA Specialty Crop Research Initiative; Hatch funds FX This research was supported by grants from the WI DATCP Specialty Crop Research Initiative Block Grant Program and the USDA NIFA Specialty Crop Research Initiative to A.J.B., J.J., and P.C.B., and Hatch funds to J.J. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 51 TC 7 Z9 7 U1 2 U2 40 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD APR 2 PY 2014 VL 9 IS 4 AR e93381 DI 10.1371/journal.pone.0093381 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AE6LC UT WOS:000334103000065 PM 24695527 ER PT J AU Huang, YZ Zhang, LZ Lai, XS Li, MX Sun, YJ Li, CJ Lan, XY Lei, CZ Zhang, CL Zhao, X Chen, H AF Huang, Yong-Zhen Zhang, Liang-Zhi Lai, Xin-Sheng Li, Ming-Xun Sun, Yu-Jia Li, Cong-Jun Lan, Xian-Yong Lei, Chu-Zhao Zhang, Chun-Lei Zhao, Xin Chen, Hong TI Transcription Factor ZBED6 Mediates IGF2 Gene Expression by Regulating Promoter Activity and DNA Methylation in Myoblasts SO SCIENTIFIC REPORTS LA English DT Article ID GROWTH-FACTOR-II; MUSCLE MASS; INSULIN; BOVINE; PIGS; QTL; IDENTIFICATION; SEQUENCES; SYSTEM; LOCUS AB Zinc finger, BED-type containing 6 (ZBED6) is an important transcription factor in placental mammals, affecting development, cell proliferation and growth. In this study, we found that the expression of the ZBED6 and IGF2 were upregulated during C2C12 differentiation. The IGF2 expression levels were negatively associated with the methylation status in beef cattle(P < 0.05). A luciferase assay for the IGF2 intron 3 and P3 promoter showed that the mutant-type 439 A-SNP-pGL3 in driving reporter gene transcription is significantly higher than that of the wild-type 439 G-SNP-pGL3 construct (P < 0.05). An over-expression assay revealed that ZBED6 regulate IGF2 expression and promote myoblast differentiation. Furthermore, knockdown of ZBED6 led to IGF2 expression change in vitro. Taken together, these results suggest that ZBED6 inhibits IGF2 activity and expression via a G to A transition disrupts the interaction. Thus, we propose that ZBED6 plays a critical role in myogenic differentiation. C1 [Huang, Yong-Zhen; Zhang, Liang-Zhi; Lai, Xin-Sheng; Li, Ming-Xun; Sun, Yu-Jia; Lan, Xian-Yong; Lei, Chu-Zhao; Zhao, Xin; Chen, Hong] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling, Shaanxi, Peoples R China. [Li, Cong-Jun] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD USA. [Zhang, Chun-Lei] Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou, Jiangsu, Peoples R China. RP Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling, Shaanxi, Peoples R China. EM chenhong1212@263.net FU National Natural Science Foundation of China [31272408, 30972080]; Agricultural Science and Technology Innovation Projects of Shaanxi Province [2012NKC01-13]; Program of National Beef Cattle and Yak Industrial Technology System [CARS-38]; National 863 Program of China [2013AA102505] FX This study was supported by the National Natural Science Foundation of China (Grant No. 31272408 and 30972080), Agricultural Science and Technology Innovation Projects of Shaanxi Province (Grant No. 2012NKC01-13), Program of National Beef Cattle and Yak Industrial Technology System (Grant No. CARS-38) and National 863 Program of China (Grant No. 2013AA102505). NR 36 TC 3 Z9 3 U1 1 U2 12 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2045-2322 J9 SCI REP-UK JI Sci Rep PD APR 2 PY 2014 VL 4 AR 4570 DI 10.1038/srep04570 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AD9JE UT WOS:000333580400006 PM 24691566 ER PT J AU Dawson-Hughes, B AF Dawson-Hughes, B. TI EXTRASKELETAL EFFECTS OF VITAMIN D SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases CY APR 02-05, 2014 CL Seville, SPAIN SP WCO, IOF, ESCEO C1 [Dawson-Hughes, B.] Tufts Univ, USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD APR PY 2014 VL 25 SU 2 MA PL5 BP S103 EP S104 PG 2 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CA1AT UT WOS:000348645700007 ER PT J AU Fielding, R AF Fielding, R. TI DEFINITION, PATHOPHYSIOLOGY, AND MANAGEMENT OF SARCOPENIA SO OSTEOPOROSIS INTERNATIONAL LA English DT Meeting Abstract CT World Congress on Osteoporosis, Osteoarthritis and Musculoskeletal Diseases CY APR 02-05, 2014 CL Seville, SPAIN SP WCO, IOF, ESCEO C1 [Fielding, R.] Tufts Univ, Jean Mayer USDA Human Nutr, Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Fielding, R.] Boston Claude D Pepper Older Amer Independence Ct, Boston, MA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD APR PY 2014 VL 25 SU 2 MA PL3 BP S103 EP S103 PG 1 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA CA1AT UT WOS:000348645700005 ER PT J AU Kibet, LC Saporito, LS Allen, AL May, EB Kleinman, PJA Hashem, FM Bryant, RB AF Kibet, Leonard C. Saporito, Louis S. Allen, Arthur L. May, Eric B. Kleinman, Peter J. A. Hashem, Fawzy M. Bryant, Ray B. TI A Protocol for Conducting Rainfall Simulation to Study Soil Runoff SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 86; Agriculture; Water Pollution; Water Quality; Technology; Industry; and Agriculture; Rainfall Simulator; Artificial Rainfall; Runoff; Packed Soil Boxes; Nonpoint Source; Urea ID COASTAL-PLAIN SOILS; POULTRY LITTER; PHOSPHORUS; EROSION; UREA AB Rainfall is a driving force for the transport of environmental contaminants from agricultural soils to surficial water bodies via surface runoff. The objective of this study was to characterize the effects of antecedent soil moisture content on the fate and transport of surface applied commercial urea, a common form of nitrogen (N) fertilizer, following a rainfall event that occurs within 24 hr after fertilizer application. Although urea is assumed to be readily hydrolyzed to ammonium and therefore not often available for transport, recent studies suggest that urea can be transported from agricultural soils to coastal waters where it is implicated in harmful algal blooms. A rainfall simulator was used to apply a consistent rate of uniform rainfall across packed soil boxes that had been prewetted to different soil moisture contents. By controlling rainfall and soil physical characteristics, the effects of antecedent soil moisture on urea loss were isolated. Wetter soils exhibited shorter time from rainfall initiation to runoff initiation, greater total volume of runoff, higher urea concentrations in runoff, and greater mass loadings of urea in runoff. These results also demonstrate the importance of controlling for antecedent soil moisture content in studies designed to isolate other variables, such as soil physical or chemical characteristics, slope, soil cover, management, or rainfall characteristics. Because rainfall simulators are designed to deliver raindrops of similar size and velocity as natural rainfall, studies conducted under a standardized protocol can yield valuable data that, in turn, can be used to develop models for predicting the fate and transport of pollutants in runoff. C1 [Kibet, Leonard C.; Allen, Arthur L.; Hashem, Fawzy M.] Univ Maryland Eastern Shore, Dept Agr Food & Resource Sci, Princess Anne, MD USA. [Saporito, Louis S.; Kleinman, Peter J. A.; Bryant, Ray B.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, Beltsville, MD 20705 USA. [May, Eric B.] Univ Maryland Eastern Shore, Dept Nat Sci, Princess Anne, MD USA. RP Bryant, RB (reprint author), ARS, Pasture Syst & Watershed Management Res Unit, USDA, Beltsville, MD 20705 USA. EM ray.bryant@ars.usda.gov FU Capacity Building Grant by the National Institute of Food and Agriculture FX This work was funded in part by a Capacity Building Grant awarded to the University of Maryland Eastern Shore (UMES) by the National Institute of Food and Agriculture. The authors would like to thank Don Mahan (UMES) for his help in setting up the rainfall simulator and in conducting rainfall simulations. Thanks are also extended to Janice Donohoe (UMES) for performing laboratory analyses and undergraduates students (UMES) for their help in conducting the rainfall simulation experiment and processing of samples. NR 18 TC 0 Z9 0 U1 5 U2 8 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD APR PY 2014 IS 86 AR e51664 DI 10.3791/51664 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA0LK UT WOS:000348609000072 ER PT J AU Barainca, P Rhodes, D Clemens, J Moshfegh, A AF Barainca, Pamela Rhodes, Donna Clemens, John Moshfegh, Alanna TI Vitamin D: intake of Americans from diet and supplements SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Barainca, Pamela; Rhodes, Donna; Clemens, John; Moshfegh, Alanna] USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 632.10 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646702283 ER PT J AU Blanche, IP Liu, C Smith, D von Lintig, J Wang, XD AF Blanche, I. P. Liu, Chun Smith, Donald von Lintig, Johannes Wang, Xiang-Dong TI Lycopene and its metabolite apo-10 '-lycopenoic acid suppressed high-fat diet-induced hepatic steatosis in beta-carotene-9 ',10 '-oxygenase knockout mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [von Lintig, Johannes] Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA. [Blanche, I. P.; Wang, Xiang-Dong] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Smith, Donald] Tufts Univ, Comparat Biol Unit, JM USDA HNRCA, Boston, MA 02111 USA. [Blanche, I. P.; Liu, Chun; Wang, Xiang-Dong] Tufts Univ, Nutr & Canc Biol Lab, JM USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 39.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701095 ER PT J AU Blanche, IP Liu, C Smith, D von Lintig, J Wang, XD AF Blanche, I. P. Liu, Chun Smith, Donald von Lintig, Johannes Wang, Xiang-Dong TI Lycopene supplementation inhibited high-fat diet-promoted hepatic tumorigenesis in both wild-type and beta-carotene-9 ',10 '-oxygenase knockout mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [von Lintig, Johannes] Case Western Reserve Univ, Sch Med, Dept Pharmacol, Cleveland, OH 44106 USA. [Blanche, I. P.; Wang, Xiang-Dong] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Smith, Donald] Tufts Univ, JM USDA HNRCA, Comparat Biol Unit, Boston, MA 02111 USA. [Blanche, I. P.; Liu, Chun; Wang, Xiang-Dong] Tufts Univ, JM USDA HNRCA, Nutr & Canc Biol Lab, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 123.1 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700052 ER PT J AU Bowman, S Friday, J Thoerig, R Clemens, J Moshfegh, A AF Bowman, Shanthy Friday, James Thoerig, Rachel Clemens, John Moshfegh, Alanna TI Americans consume less added sugars and solid fats and consume more whole grains and oils: changes from 2003-04 to 2009-10 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Bowman, Shanthy; Friday, James; Thoerig, Rachel; Clemens, John; Moshfegh, Alanna] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 369.2 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700486 ER PT J AU Cao, HP Zhang, L Tan, XF Long, HX Shockey, J AF Cao, Heping Zhang, Lin Tan, Xiaofeng Long, Hongxu Shockey, Jay TI Identification, classification and differential expression of oleosin genes in tung tree SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Zhang, Lin; Tan, Xiaofeng; Long, Hongxu] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China. [Cao, Heping; Shockey, Jay] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 605.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646702110 ER PT J AU Casperson, S Schoeller, D Johnson, LA Whigham, L AF Casperson, Shanon Schoeller, Dale Johnson, LuAnn Whigham, Leah TI Calculation of total meal delta 13C from individual food delta 13C SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Schoeller, Dale] Univ Wisconsin, Madison, WI USA. [Casperson, Shanon; Johnson, LuAnn; Whigham, Leah] ARS, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 813.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704440 ER PT J AU Claycombe, K Roemmich, J Johnson, W AF Claycombe, Kate Roemmich, James Johnson, W. TI Maternal low protein diet induces muscle mitochondrial respiration impairment, catch-up growth and PGC-1 alpha expression SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Claycombe, Kate; Roemmich, James; Johnson, W.] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 271.6 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700380 ER PT J AU Dashti, H Smith, C Tanaka, T Ordovas, J AF Dashti, Hassan Smith, Caren Tanaka, Toshiko Ordovas, Jose TI Interactions between circadian gene variants and fatty acid intake for metabolic syndrome risk: a meta-analysis from the Cohorts for Heart and Aging Research in Genomic Epidemiology Consortium SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Dashti, Hassan; Smith, Caren; Ordovas, Jose] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tanaka, Toshiko] NIA, Translat Gerontol Branch, Baltimore, MD 21224 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 373.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701028 ER PT J AU El-Abbadi, N Jacques, P Lin, HH Rogers, G Troy, L AF El-Abbadi, Naglaa Jacques, Paul Lin, Honghuang Rogers, Gail Troy, Lisa TI The 2010 Dietary Guidelines for Americans food groups associated with decreased inflammation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Lin, Honghuang] Boston Univ, Sch Med, Dept Med, Sect Computat Biomed, Boston, MA 02118 USA. [El-Abbadi, Naglaa; Jacques, Paul; Rogers, Gail] Tufts Univ, Jean Mayer USDA HNRCA, Boston, MA 02111 USA. [Troy, Lisa] Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Amherst, MA 01003 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 370.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701014 ER PT J AU Ghanem, EB Wu, DY Camilli, A Leong, J Meydani, S AF Ghanem, Elsa Bou Wu, Dayong Camilli, Andrew Leong, John Meydani, Simin TI Vitamin E boosts resistance to Streptococcus pneumoniae infection in aged mice by inhibiting hepoxilin A3-mediated neutrophil recruitment across the lung epithelium SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Camilli, Andrew] Howard Hughes Med Inst, Boston, MA 02115 USA. [Wu, Dayong; Meydani, Simin] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ghanem, Elsa Bou; Camilli, Andrew; Leong, John] Tufts Univ, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 392.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701114 ER PT J AU Goodman, M Thomson, J Tussing-Humphreys, L AF Goodman, Melissa Thomson, Jessica Tussing-Humphreys, Lisa TI Baseline characteristics of a pregnant, primarily African American cohort residing in the Mississippi Delta: Delta Healthy Sprouts SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Tussing-Humphreys, Lisa] Univ Illinois, Dept Med, Chicago, IL USA. [Goodman, Melissa; Thomson, Jessica] ARS, USDA, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 806.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704390 ER PT J AU Gurzell, E Wiesinger, J Harris, W Fenton, J AF Gurzell, Eric Wiesinger, Jason Harris, William Fenton, Jenifer TI Is the omega-3 index a valid marker of intestinal membrane phospholipid EPA plus DHA content? SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Fenton, Jenifer] Michigan State Univ, Coll Osteopath Med, E Lansing, MI 48824 USA. [Gurzell, Eric; Fenton, Jenifer] Michigan State Univ, E Lansing, MI 48824 USA. [Harris, William] Univ S Dakota, Sanford Sch Med, Sioux Falls, SD USA. [Wiesinger, Jason] ARS, USDA, E Lansing, MI USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 822.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704491 ER PT J AU Gusev, P Andrews, K Palachuvattil, J Dang, P Roseland, J Holden, J Savarala, S Pehrsson, P Dwyer, J Betz, J Saldanha, L Bailey, R Costello, R Gahche, J Hardy, C Emenaker, N Douglass, L AF Gusev, P. Andrews, K. Palachuvattil, J. Dang, P. Roseland, J. Holden, J. Savarala, S. Pehrsson, P. Dwyer, J. Betz, J. Saldanha, L. Bailey, R. Costello, R. Gahche, J. Hardy, C. Emenaker, N. Douglass, L. TI Over-the-counter prenatal multivitamin/mineral products: chemical analysis for the Dietary Supplement Ingredient Database SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Gahche, J.] CDC, NHANES, NCHS, Hyattsville, MD USA. [Douglass, L.] Consulting Statistician, Longmont, CO USA. [Hardy, C.] FDA, CFSAN, College Pk, MD USA. [Emenaker, N.] NCI, NIH, Bethesda, MD 20892 USA. [Dwyer, J.; Betz, J.; Saldanha, L.; Bailey, R.; Costello, R.] NIH, ODS, Bethesda, MD 20892 USA. [Gusev, P.; Andrews, K.; Palachuvattil, J.; Dang, P.; Roseland, J.; Holden, J.; Savarala, S.; Pehrsson, P.] ARS, BHNRC, NDL, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 809.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704408 ER PT J AU Hacker, A King, J Van Loan, M Gertz, E Adams, M Sawyer, A Fung, E AF Hacker, Andrea King, Janet Van Loan, Marta Gertz, Erik Adams, Mickye Sawyer, Aenor Fung, Ellen TI Calcium supplementation during pregnancy improves tibial bone density at one year post-partum in racially diverse women SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Adams, Mickye] Alameda Cty Med Ctr, Oakland, CA USA. [King, Janet; Fung, Ellen] Childrens Hosp & Res Ctr, Oakland, CA USA. [Hacker, Andrea] Missouri Southern State Univ, Joplin, MO USA. [Sawyer, Aenor] Univ Calif San Francisco, San Francisco, CA 94143 USA. [Van Loan, Marta; Gertz, Erik] USDA, Western Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 250.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700298 ER PT J AU Harrison, G McNutt, S Weinfield, N MacAllum, C Owens, T AF Harrison, Gail McNutt, Suzanne Weinfield, Nancy MacAllum, Crystal Owens, Tameka TI National WIC infants and toddlers feeding study SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Harrison, Gail] Univ Calif Los Angeles, Los Angeles, CA USA. [Owens, Tameka] USDA, Off Policy Support, Alexandria, VA USA. [McNutt, Suzanne; Weinfield, Nancy; MacAllum, Crystal] Westat Corp, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 805.15 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704379 ER PT J AU Hawkes, W Alkan, Z Duong, F Rangan, P AF Hawkes, Wayne Alkan, Zeynep Duong, Frank Rangan, Priya TI Selenoprotein W regulates epidermal growth factor receptor trafficking, expression, and activation SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Hawkes, Wayne; Alkan, Zeynep; Duong, Frank; Rangan, Priya] USDA WHNRC, Davis, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 374.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701038 ER PT J AU Herman, D Afulani, P Harrison, G Coleman-Jensen, A AF Herman, Dena Afulani, Patience Harrison, Gail Coleman-Jensen, Alisha TI Delaying and reducing medication use as a coping mechanism for food insecurity SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Herman, Dena; Afulani, Patience; Harrison, Gail] Univ Calif Los Angeles, Fielding Sch Publ Hlth, Los Angeles, CA USA. [Coleman-Jensen, Alisha] USDA, Econ Res Serv, Washington, DC USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 268.6 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700364 ER PT J AU Holt, R Yim, SJ Shearer, G Keen, C Djurica, D Newman, J Shindel, A Hackman, R AF Holt, Roberta Yim, Sun Jung Shearer, Gregory Keen, Carl Djurica, Dragana Newman, John Shindel, Alan Hackman, Robert TI Correlation of lipoprotein epoxide content to microvascular function after short-term walnut intake SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Shearer, Gregory] Penn State Univ, University Pk, PA 16802 USA. [Holt, Roberta; Yim, Sun Jung; Keen, Carl; Djurica, Dragana; Newman, John; Hackman, Robert] Univ Calif Davis, Davis, CA 95616 USA. [Shindel, Alan] Univ Calif Davis, Sacramento, CA 95817 USA. [Newman, John] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 831.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646705042 ER PT J AU Hoy, M Sebastian, R Enns, C Goldman, J Moshfegh, A AF Hoy, M. Sebastian, Rhonda Enns, Cecilia Goldman, Joseph Moshfegh, Alanna TI Contribution of sandwiches to USDA food patterns components, What We Eat In America, NHANES 2009-2010 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Hoy, M.; Sebastian, Rhonda; Enns, Cecilia; Goldman, Joseph; Moshfegh, Alanna] ARS, Food Surveys Res Grp, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 393.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701118 ER PT J AU Hoy, M Goldman, J Bowman, S Moshfegh, A AF Hoy, M. Goldman, Joseph Bowman, Shanthy Moshfegh, Alanna TI Foods contributing to fruit and vegetable intakes of US adults: What We Eat In America, NHANES 2009-2010 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Hoy, M.; Goldman, Joseph; Bowman, Shanthy; Moshfegh, Alanna] USDA, Food Surveys Res Grp, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 369.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700487 ER PT J AU Huang, HQ Yu, LG Wang, T AF Huang, Haiqiu Yu, Liangli Wang, Thomas TI Expression of the xenobiotic metabolizing enzyme cytochrome P450 1B1 alters anti-inflammatory activity of quercetin, kaempferol and taxifolin in macrophage and monocyte SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Huang, Haiqiu; Yu, Liangli] Univ Maryland, College Pk, MD 20742 USA. [Wang, Thomas] USDA ARS, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 830.25 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646705033 ER PT J AU Jang, S Molokin, A Urban, J Solano-Aguilar, G AF Jang, Saebyeol Molokin, Aleksey Urban, Jeseph, Jr. Solano-Aguilar, Gloria TI Effects of fat sources on high fat induced changes in inflammation and lipid metabolism in juvenile pigs SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Jang, Saebyeol; Molokin, Aleksey; Urban, Jeseph, Jr.; Solano-Aguilar, Gloria] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 822.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704492 ER PT J AU Kim, H Bartley, G Chon, JW Seo, KH Yokoyama, W AF Kim, Hyunsook Bartley, Glenn Chon, Jung-Whan Seo, Kun-Ho Yokoyama, Wallace TI Chardonnay grape seed flour alters expression of hepatic genes for lipid and glucose metabolism, inflammation, and leptin receptor SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Chon, Jung-Whan; Seo, Kun-Ho] Konkuk Univ, Publ Hlth 3Dept, Seoul, South Korea. [Kim, Hyunsook] Konkuk Univ, Coll Vet Med, Dept Physiol, Seoul, South Korea. [Kim, Hyunsook] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Bartley, Glenn; Yokoyama, Wallace] USDA, Western Reg Res Ctr, Albany, CA 94710 USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 829.13 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646705021 ER PT J AU Kim, J Pedersen, T Newman, J Watkins, B AF Kim, Jeffrey Pedersen, Theresa Newman, John Watkins, Bruce TI Dietary DHA supports glucose use by muscle in association with improved endocannabinoid system gene expression in C57/blk6 mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Kim, Jeffrey; Watkins, Bruce] Univ Connecticut, Ctr Aging, Farmington, CT USA. [Pedersen, Theresa; Newman, John] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 248.7 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700292 ER PT J AU Meydani, S Das, S Piper, C Lewis, M Dixit, V Gupta, A Villareal, D Klein, S Bhapkar, M Huang, M Fuss, P Roberts, S Holloszy, J Fontana, L AF Meydani, Simin Das, Sai Piper, Carl Lewis, Michael Dixit, Vishwadeep Gupta, Alok Villareal, Dennis Klein, Samuel Bhapkar, Manjushri Huang, Megan Fuss, Paul Roberts, Susan Holloszy, John Fontana, Luigi TI Effects of prolonged calorie restriction on inflammation and immune function: a randomized controlled trial in non-obese humans SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Piper, Carl; Bhapkar, Manjushri; Huang, Megan] Duke Clin Res Inst, Durham, NC USA. [Meydani, Simin; Das, Sai; Fuss, Paul; Roberts, Susan] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. [Dixit, Vishwadeep; Gupta, Alok] Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Lewis, Michael] Univ Vermont, Burlington, VT USA. [Villareal, Dennis; Klein, Samuel; Holloszy, John; Fontana, Luigi] Washington Univ, Sch Med, St Louis, MO USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 40.4 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701143 ER PT J AU Moshfegh, A Parker, E Martin, C Adler, M Hoy, M Rhodes, D Anand, J Montville, J Sebastian, R AF Moshfegh, Alanna Parker, Elizabeth Martin, Carrie Adler, Meghan Hoy, Mary Rhodes, Donna Anand, Jaswinder Montville, Janice Sebastian, Rhonda TI Capturing sodium reduction efforts in What We Eat in America, NHANES SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Moshfegh, Alanna; Parker, Elizabeth; Martin, Carrie; Adler, Meghan; Hoy, Mary; Rhodes, Donna; Anand, Jaswinder; Montville, Janice; Sebastian, Rhonda] USDA, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 384.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701082 ER PT J AU Olanrewaju, H Purswell, J Collier, S Branton, S AF Olanrewaju, Hammed Purswell, Joseph Collier, Stephanie Branton, Scott TI Effects of color temperature (Kelvin) of LED bulbs on blood physiological variables of broilers grown to heavy weights SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Olanrewaju, Hammed; Purswell, Joseph; Collier, Stephanie; Branton, Scott] ARS, USDA, Mississippi State, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 674.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646703087 ER PT J AU Panagos, P Matthan, N Sen, S AF Panagos, Patoula Matthan, Nirupa Sen, Sarbattama TI Effects of maternal obesity on breastmilk composition and infant growth SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Matthan, Nirupa; Sen, Sarbattama] Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA USA. [Sen, Sarbattama] Mother Infant Res Inst, Boston, MA USA. [Panagos, Patoula; Sen, Sarbattama] Tufts Med Ctr, Boston, MA USA. NR 0 TC 2 Z9 2 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 247.7 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700288 ER PT J AU Parker, E Goldman, J Moshfegh, A AF Parker, Elizabeth Goldman, Joseph Moshfegh, Alanna TI America's nutrition report card: comparing WWEIA, NHANES 2007-2010 usual nutrient intakes to dietary reference intakes SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Parker, Elizabeth; Goldman, Joseph; Moshfegh, Alanna] USDA, Food Surveys Res Grp, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 384.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701078 ER PT J AU Pfalzer, A Leung, K Garcia, P Liu, ZH Mason, J AF Pfalzer, Anna Leung, Keith Garcia, Paloma Liu, Zhenhua Mason, Joel TI Human obesity produces an inflammatory milieu in both the serum and colonic mucosa: implications for carcinogenesis SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Leung, Keith] Tufts Med Ctr, Boston, MA USA. [Pfalzer, Anna] Tufts Univ, Boston, MA 02111 USA. [Garcia, Paloma; Mason, Joel] Tufts Univ, HNRCA, Boston, MA 02111 USA. [Liu, Zhenhua] Univ Massachusetts, Amherst, MA 01003 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 136.6 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700103 ER PT J AU Piccolo, B Newman, J Gertz, E Woodhouse, L Souza, E Gustafson, M Sheets, C Fiehn, O Keim, N Adams, S Van Loan, M AF Piccolo, Brian Newman, John Gertz, Erik Woodhouse, Leslie Souza, Elaine Gustafson, Mary Sheets, Caitlin Fiehn, Oliver Keim, Nancy Adams, Sean Van Loan, Marta TI Clinical and metabolomic predictors of weight loss in overweight and obese adults in an energy-restricted controlled feeding trial SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Newman, John; Souza, Elaine; Sheets, Caitlin; Keim, Nancy; Adams, Sean; Van Loan, Marta] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Piccolo, Brian; Fiehn, Oliver] Univ Calif Davis, West Coast Metabol Ctr, Davis, CA 95616 USA. [Newman, John; Gertz, Erik; Woodhouse, Leslie; Gustafson, Mary; Keim, Nancy; Adams, Sean; Van Loan, Marta] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 248.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700291 ER PT J AU Plundrich, N White, B Davis, J Lila, MA AF Plundrich, Nathalie White, Brittney Davis, Jack Lila, Mary Ann TI Evaluation of protein-phytochemical interactions in a hypoallergenic edible peanut matrix SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Plundrich, Nathalie; Lila, Mary Ann] N Carolina State Univ, Raleigh, NC 27695 USA. [White, Brittney] ARS, Qual & Handling Res Unit 2Market, USDA, Raleigh, NC USA. [Davis, Jack] ARS, Market Qual & Handling Res Unit, USDA, Raleigh, NC USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 813.10 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704436 ER PT J AU Raatz, S Vickers, Z Strubeck, E Johnson, L AF Raatz, Susan Vickers, Zata Strubeck, Eric Johnson, LuAnn TI Development of a standard methodology for assessing the satiating effect of foods SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Raatz, Susan; Vickers, Zata] Univ Minnesota, St Paul, MN 55108 USA. [Raatz, Susan; Strubeck, Eric; Johnson, LuAnn] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 47.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701169 ER PT J AU Rafacho, BP Stice, C Liu, C Ausman, L Greenberg, A Wang, XD AF Rafacho, Bruna Paola Stice, Camilla Liu, Chun Ausman, Lynne Greenberg, Andrew Wang, Xiang-Dong TI Flavonoid luteolin supplementation inhibits diethylnitrosamine-initiated alcohol-promoted hepatic inflammation and precancerous lesions in mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Rafacho, Bruna Paola; Stice, Camilla; Liu, Chun; Ausman, Lynne; Greenberg, Andrew; Wang, Xiang-Dong] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 829.7 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646705029 ER PT J AU Rhodes, D Parker, E Goldman, J Gaine, PC Moshfegh, A AF Rhodes, Donna Parker, Elizabeth Goldman, Joseph Gaine, P. Coutney Moshfegh, Alanna TI Sodium to potassium ratio and food choices of US adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Gaine, P. Coutney] Int Life Sci Inst, Washington, DC USA. [Rhodes, Donna; Parker, Elizabeth; Goldman, Joseph; Moshfegh, Alanna] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 384.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701083 ER PT J AU Roe, A Zhang, SC Crivello, N Zhao, XQ Johnson, E Lichtenstein, A Smith, C Zeisel, S Rosenberg, I Scott, T AF Roe, Annie Zhang, Shucha Crivello, Natalia Zhao, Xueqing Johnson, Elizabeth Lichtenstein, Alice Smith, Caren Zeisel, Steven Rosenberg, Irwin Scott, Tammy TI Choline and choline-related compounds are associated with cognitive performance and cerebrovascular pathology in older adults SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Roe, Annie; Crivello, Natalia; Johnson, Elizabeth; Lichtenstein, Alice; Smith, Caren; Rosenberg, Irwin; Scott, Tammy] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Zhang, Shucha; Zhao, Xueqing; Zeisel, Steven] Univ North Carolina Chapel Hill, Nutr Res Inst, Kannapolis, NC USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 135.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700096 ER PT J AU Roemmich, J AF Roemmich, James TI Effect of height adjustable desks on standing time, discomfort, and productivity of office workers with sedentary occupations SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Roemmich, James] ARS, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 2 U2 8 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 37.8 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701011 ER PT J AU Rust, B Horn, W Cooper, D Welch, L Martin, R Pelkman, C Kim, EB Marco, M Keim, N AF Rust, Bret Horn, William Cooper, Danielle Welch, Lucas Martin, Roy Pelkman, Christine Kim, Eun Bae Marco, Maria Keim, Nancy TI Does production of methane in the gut affect glucose tolerance? SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Pelkman, Christine] Ingredion Inc, Bridgewater, NJ USA. [Rust, Bret; Cooper, Danielle; Martin, Roy; Kim, Eun Bae; Marco, Maria; Keim, Nancy] Univ Calif Davis, Davis, CA USA. [Rust, Bret; Horn, William; Welch, Lucas; Keim, Nancy] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 822.10 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704488 ER PT J AU Sabet, J Mason, J Bronson, R Crott, J AF Sabet, Julia Mason, Joel Bronson, Rodrick Crott, Jimmy TI Modulation of paternal B vitamin intake does not affect tumorigenesis but does alter growth trajectory in Apc(1638N) mouse offspring SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Sabet, Julia; Mason, Joel; Crott, Jimmy] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Bronson, Rodrick] Harvard Univ, Sch Med, Rodent Histopathol Core, Boston, MA USA. [Sabet, Julia; Mason, Joel; Crott, Jimmy] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 137.7 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700112 ER PT J AU Saldanha, L Dwyer, J Bailen, R Andrews, K Bailey, R Betz, J Burt, V Chang, F Costello, R Emenaker, N Gahche, J Harnly, J Hardy, C Pehrsson, P AF Saldanha, L. Dwyer, J. Bailen, R. Andrews, K. Bailey, R. Betz, J. Burt, V. Chang, F. Costello, R. Emenaker, N. Gahche, J. Harnly, J. Hardy, C. Pehrsson, P. TI When a dietary supplement product name says "energy", what's in the bottle? SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Andrews, K.; Harnly, J.; Pehrsson, P.] USDA ARS, Beltsville, MD USA. [Hardy, C.] US FDA, CFSAN, College Pk, MD USA. [Emenaker, N.] NCI, NIH, Rockville, MD USA. [Burt, V.; Gahche, J.] CDC, NHANES, Hyattsville, MD USA. [Chang, F.] Natl Lib Med, NIH, Bethesda, MD USA. [Saldanha, L.; Dwyer, J.; Bailen, R.; Bailey, R.; Betz, J.; Costello, R.] NIH, ODS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 634.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646702298 ER PT J AU Sawaengsri, H Wang, JP Reginaldo, C Smith, D Wu, DY Meydani, S Selhub, J Paul, L AF Sawaengsri, Hathairat Wang, Junpeng Reginaldo, Christina Smith, Donald Wu, Dayong Meydani, Simin Selhub, Jacob Paul, Ligi TI Reduced IL-10 production contributes to impaired NK cytotoxicity in old mice fed a high folic acid diet SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Sawaengsri, Hathairat; Reginaldo, Christina] Friedman Sch Nutr Sci & Policy, Boston, MA USA. [Sawaengsri, Hathairat; Wang, Junpeng; Reginaldo, Christina; Smith, Donald; Wu, Dayong; Meydani, Simin; Selhub, Jacob; Paul, Ligi] Tufts Univ, JM USDA HNRCA, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 638.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646702325 ER PT J AU Sebastian, R Goldman, J Enns, CW Martin, C Steinfeldt, L Dwyer, J Moshfegh, A Milner, J AF Sebastian, Rhonda Goldman, Joseph Enns, Cecilia Wilkinson Martin, Carrie Steinfeldt, Lois Dwyer, Johanna Moshfegh, Alanna Milner, John TI Food sources of flavonoids for adults in the United States: What We Eat in America, NHANES 2007-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Sebastian, Rhonda; Goldman, Joseph; Enns, Cecilia Wilkinson; Martin, Carrie; Steinfeldt, Lois; Moshfegh, Alanna; Milner, John] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. [Dwyer, Johanna] NIH, Off Dietary Supplements, DHHS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 369.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700489 ER PT J AU Sebastian, R Goldman, J Enns, CW Martin, C Steinfeldt, L Dwyer, J Moshfegh, A Milner, J AF Sebastian, Rhonda Goldman, Joseph Enns, Cecilia Wilkinson Martin, Carrie Steinfeldt, Lois Dwyer, Johanna Moshfegh, Alanna Milner, John TI Flavonoid intakes by adults in the United States: What We Eat in America, NHANES 2007-2008 SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Sebastian, Rhonda; Goldman, Joseph; Enns, Cecilia Wilkinson; Martin, Carrie; Steinfeldt, Lois; Moshfegh, Alanna; Milner, John] ARS, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD USA. [Dwyer, Johanna] NIH, Off Dietary Supplements, DHHS, Bethesda, MD 20892 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 245.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700272 ER PT J AU Shahab-Ferdows, S Engle-Stone, R Hampel, D Ndjebayi, A Nankap, M Brown, K Allen, L AF Shahab-Ferdows, Setareh Engle-Stone, Reina Hampel, Daniela Ndjebayi, Alex Nankap, Martin Brown, Kenneth Allen, Lindsay TI Risk factors for folate deficiency differ from those for vitamin B12 deficiency in Cameroonian women and children SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Shahab-Ferdows, Setareh; Hampel, Daniela; Allen, Lindsay] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA USA. [Ndjebayi, Alex; Nankap, Martin] Helen Keller Int, Yaounde, Cameroon. [Engle-Stone, Reina; Brown, Kenneth] Univ Calif Davis, Davis, CA 95616 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 119.7 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700034 ER PT J AU Solano-Aguilar, G Conchas, A Molokin, A Jang, S Lakshman, S Santin-Duran, M Sikaroodi, M Gillevet, P Urban, J AF Solano-Aguilar, Gloria Conchas, Andrew Molokin, Aleksey Jang, Saebyeol Lakshman, Sukla Santin-Duran, Monica Sikaroodi, Masoumeh Gillevet, Patrick Urban, Joseph, Jr. TI The source of dietary fat modulates the intestinal microbiota and liver metabolite pattern SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Sikaroodi, Masoumeh; Gillevet, Patrick] George Mason Univ, Fairfax, VA 22030 USA. [Solano-Aguilar, Gloria; Conchas, Andrew; Molokin, Aleksey; Jang, Saebyeol; Lakshman, Sukla; Santin-Duran, Monica; Urban, Joseph, Jr.] ARS, USDA, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 822.6 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646704494 ER PT J AU Stoody, E McGrane, M MacNeil, P Altman, J Fungwe, T Lyon, J Obbagy, J Wong, YP Spahn, J AF Stoody, Eve McGrane, Mary MacNeil, Patricia Altman, Jean Fungwe, Thomas Lyon, Joan Obbagy, Julie Wong, Yat Ping Spahn, Joanne TI Dietary patterns and risk of cardiovascular disease: a systematic review SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Stoody, Eve; McGrane, Mary; MacNeil, Patricia; Altman, Jean; Fungwe, Thomas; Lyon, Joan; Obbagy, Julie; Wong, Yat Ping; Spahn, Joanne] USDA, Ctr Nutr Policy & Promot, Alexandria, VA USA. NR 0 TC 1 Z9 1 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 628.14 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646702258 ER PT J AU Suryawan, A Nguyen, H Parada, R Davis, T AF Suryawan, Agus Nguyen, Hanh Parada, Rosemarie Davis, Teresa TI Regulation of the protein degradation pathways by amino acids and insulin in skeletal muscle of neonatal pigs SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Suryawan, Agus; Nguyen, Hanh; Parada, Rosemarie; Davis, Teresa] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 137.1 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700106 ER PT J AU Thomson, J Tussing-Humphreys, L AF Thomson, Jessica Tussing-Humphreys, Lisa TI A church-based, diet and supervised physical activity behavioral intervention for rural, southern African American adults improves diet and physical activity-related psychosocial constructs SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Tussing-Humphreys, Lisa] Univ Illinois, Dept Med, Chicago, IL USA. [Thomson, Jessica] ARS, USDA, Stoneville, MS USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 624.1 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646702238 ER PT J AU Unger, V Pope, C Flores, A DeFeo, C AF Unger, Vinzenz Pope, Christopher Flores, Adrian DeFeo, Christopher TI In the shadows of the membrane: A tale about how to beat the odds in cellular copper acquisition SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Unger, Vinzenz; Pope, Christopher; Flores, Adrian] Northwestern Univ, Dept Mol Biosci, Evanston, IL USA. [DeFeo, Christopher] USDA, Ctr Biol Evaluat & Res, Bethesda, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 360.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700476 ER PT J AU Wang, Y Gapstur, S Gaudet, M Peterson, J Dwyer, J McCullough, M AF Wang, Ying Gapstur, Susan Gaudet, Mia Peterson, Julia Dwyer, Johanna McCullough, Marjorie TI Dietary flavonoid intake and breast cancer risk by hormone receptor status among postmenopausal women in a prospective US cohort SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Wang, Ying; Gapstur, Susan; Gaudet, Mia; McCullough, Marjorie] Epidemiol Amer Canc Soc, Atlanta, GA USA. [Peterson, Julia] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Dwyer, Johanna] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Dwyer, Johanna] Tufts Univ, Jean Mayer USDA Human Nutr Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 267.7 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646700361 ER PT J AU Zeng, HW AF Zeng, Huawei TI Methylselenol, a selenium metabolite, inhibits colon cancer cell growth and cancer xenografts in C57BL/6 mice SO FASEB JOURNAL LA English DT Meeting Abstract CT Experimental Biology Meeting CY APR 26-30, 2014 CL San Diego, CA SP Cenveo, LI COR, Wiley, Mead Johnson Pediat Nutr Inst, IPRECIO, F1000 Res, Amer Assoc Anatomists, Amer Physiol Soc, Amer Soc Biochem & Mol Biol, Amer Soc Investigat Pathol, Amer Soc Nutr, Amer Soc Pharmacol & Expt Therapeut C1 [Zeng, Huawei] ARS, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 374.2 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0MP UT WOS:000346646701036 ER PT J AU Burley, JD Bytnerowicz, A Ray, JD Schilling, S Allen, EB AF Burley, Joel D. Bytnerowicz, Andrzej Ray, John D. Schilling, Susan Allen, Edith B. TI Surface ozone in Joshua Tree National Park SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Portable ozone monitor; Passive samplers; Spatial interpolation; HYSPLIT model; Ozone exposure indices ID WESTERN UNITED-STATES; TROPOSPHERIC OZONE; REGIONAL PATTERNS; FORESTS; CALIFORNIA; MOUNTAINS; REGIMES; PLANTS AB Surface ozone concentrations are high throughout Joshua Tree National Park (JOTR), as demonstrated by direct monitoring at multiple sites within JOTR. Multiple years of data from continuous ozone monitors and passive samplers indicate that the highest ozone concentrations occur in the northwest corner of JOTR, and decrease as one moves to the south and east. Analysis of long-term (1994-2010) hourly data from the Black Rock monitoring station indicates that average summertime ozone concentrations have remained essentially constant, with a concurrent decrease in the number, and magnitude, of high ozone events. Calculation of ozone exposure indices suggests that ozone concentrations may be high enough to adversely affect local flora, especially in the northwestern region of the park. Back-trajectory analysis using the HYSPLIT model suggests that most of the ozone measured in JOTR is transported into the park from a high source area (Los Angeles and surrounding suburbs) to the west. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Burley, Joel D.] St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. [Bytnerowicz, Andrzej; Schilling, Susan] US Forest Serv, USDA, Pacific Southwest Res Stn, Riverside, CA 92507 USA. [Ray, John D.] Natl Pk Serv, Air Resources Div, Denver, CO 80225 USA. [Allen, Edith B.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Allen, Edith B.] Univ Calif Riverside, Ctr Conservat Biol, Riverside, CA 92521 USA. RP Burley, JD (reprint author), St Marys Coll Calif, Dept Chem, Moraga, CA 94575 USA. EM jburley@stmarys-ca.edu NR 31 TC 3 Z9 3 U1 0 U2 8 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD APR PY 2014 VL 87 BP 95 EP 107 DI 10.1016/j.atmosenv.2013.12.043 PG 13 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AY2QR UT WOS:000347434900012 ER PT J AU Ahuja, J Pehrsson, P Haytowitz, D Showell, B Nickle, M Wasswa-Kintu, S Cogswell, M AF Ahuja, Jaspreet Pehrsson, Pamela Haytowitz, David Showell, Bethany Nickle, Melissa Wasswa-Kintu, Shirley Cogswell, Mary TI Comparison of corresponding commercially processed foods from store and restaurant for sodium, fat, sugar and potassium content in a nationwide sample SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Cogswell, Mary] Ctr Dis Control & Prevent, Atlanta, GA USA. [Ahuja, Jaspreet; Pehrsson, Pamela; Haytowitz, David; Showell, Bethany; Nickle, Melissa; Wasswa-Kintu, Shirley] ARS, USDA, Beltsville Human Nutr Res Ctr, Beltsville, MD USA. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1019.11 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000121 ER PT J AU Barrack, M Nichols, J Rauh, M Van Loan, M AF Barrack, Michelle Nichols, Jeanne Rauh, Mitchell Van Loan, Marta TI Evidence of energy deficiency, using doubly-labeled water, among adolescent endurance runners SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Barrack, Michelle] Calif State Univ Northridge, Northridge, CA 91330 USA. [Nichols, Jeanne; Rauh, Mitchell] San Diego State Univ, San Diego, CA 92182 USA. [Van Loan, Marta] USDA, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA LB316 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651004344 ER PT J AU Beavers, A Scott, P Lauter, AM Goggi, S Reddy, M AF Beavers, Alyssa Scott, Paul Lauter, Adrienne Moran Goggi, Susana Reddy, Manju TI Iron bioavailability of low and high phytic aid maize produced via recurrent selection SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Beavers, Alyssa; Reddy, Manju] Iowa State Univ, Ames, IA USA. [Goggi, Susana] Iowa State Univ, Seed Sci Ctr, Ames, IA USA. [Scott, Paul; Lauter, Adrienne Moran] Iowa State Univ Sci & Technol, USDA ARS, Ames, IA 50011 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1042.4 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000283 ER PT J AU Demmer, E Gertz, E Rogers, T Hillgonds, D Garrod, M Van Loan, M AF Demmer, Elieke Gertz, Erik Rogers, Tara Hillgonds, Darren Garrod, Marge Van Loan, Marta TI Effect of dairy foods vs. calcium and vitamin-D supplements on bone metabolism: use of 41Ca tracer SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Hillgonds, Darren] Lawrence Livermore Natl Lab, Livermore, CA USA. [Demmer, Elieke; Rogers, Tara] Univ Calif Davis, Grad Grp Nutr Biol, Davis, CA 95616 USA. [Gertz, Erik; Garrod, Marge; Van Loan, Marta] USDA ARS, Western Human Nutr Res Ctr, Davis, CA USA. NR 0 TC 1 Z9 1 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1018.12 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000111 ER PT J AU Ghattas, H Sahyoun, N Sassine, A Barbour, J Seyfert, K Hwalla, N Nord, M AF Ghattas, Hala Sahyoun, Nadine Sassine, AnnieBelle Barbour, Jessica Seyfert, Karin Hwalla, Nahla Nord, Mark TI Household food insecurity is associated with childhood stunting in vulnerable populations in Lebanon SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Ghattas, Hala; Sassine, AnnieBelle; Barbour, Jessica; Hwalla, Nahla] Amer Univ Beirut, Beirut, Lebanon. [Seyfert, Karin] Univ London, Sch Oriental & African Studies, London, England. [Nord, Mark] USDA, ERS, Washington Dc, DC USA. [Sahyoun, Nadine] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD USA. RI Ghattas, Hala/K-5661-2016 NR 0 TC 0 Z9 0 U1 0 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1014.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000089 ER PT J AU Ip, B Liu, C Smith, D Wang, XD AF Ip, Blanche Liu, Chun Smith, Donald Wang, Xiang-Dong TI High refined carbohydrates diet promotes comparable hepatic tumorigenesis in mice as high fat diet, potentially through inducing endoplasmic reticulum stress PKR-like kinase signaling SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Ip, Blanche; Wang, Xiang-Dong] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Smith, Donald] Tufts Univ, JM USDA HNRCA, Comparat Biol Unit, Boston, MA 02111 USA. [Ip, Blanche; Liu, Chun; Wang, Xiang-Dong] Tufts Univ, JM USDA HNRCA, Nutrit & Canc Biol Lab, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1029.1 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000196 ER PT J AU Jahns, L Johnson, L AF Jahns, Lisa Johnson, LuAnn TI Seasonality in women's dietary intake: preliminary results from the Life in all Seasons study SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Jahns, Lisa; Johnson, LuAnn] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. NR 0 TC 0 Z9 0 U1 2 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1022.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000149 ER PT J AU Kirn, D Laussen, J Hau, C Reid, K Fielding, R AF Kirn, Dylan Laussen, Jonathan Hau, Cynthia Reid, Kieran Fielding, Roger TI Association of vitamin D level, leg extensor power, and neuromuscular function in mobility-limited older adults SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Kirn, Dylan; Laussen, Jonathan; Hau, Cynthia; Reid, Kieran; Fielding, Roger] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab Jean Mayer, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 863.7 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651003010 ER PT J AU MacKay, D Eck, P Baer, D Jones, P AF MacKay, Dylan Eck, Peter Baer, David Jones, Peter TI The CETP polymorphism rs5882 is associated with triglyceride lowering in response to plant sterol consumption SO FASEB JOURNAL LA English DT Meeting Abstract C1 [MacKay, Dylan; Eck, Peter] Univ Manitoba, Winnipeg, MB, Canada. [Jones, Peter] Univ Manitoba, RCFFN, Winnipeg, MB, Canada. [Baer, David] USDA, Food Components & Hlth Lab, Beltsville, MD 20705 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1038.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000253 ER PT J AU Pahlavani, M Kalupahana, N LeMieux, M Aljawadi, A Scoggin, S Claycombe, K Moustaid-Moussa, N AF Pahlavani, Mandanna Kalupahana, Nishan LeMieux, Monique Aljawadi, Arwa Scoggin, Shane Claycombe, Kate Moustaid-Moussa, Naima TI Eicosapentaenoic acid regulates brown adipose tissue gene expression and metabolism in high fat fed mice SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Pahlavani, Mandanna; LeMieux, Monique; Aljawadi, Arwa; Scoggin, Shane; Moustaid-Moussa, Naima] Texas Tech Univ, Lubbock, TX 79409 USA. [Kalupahana, Nishan] Univ Peradeniya, Peradeniya, Sri Lanka. [Claycombe, Kate] USDA ARS, GFHNRC, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1037.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000248 ER PT J AU Picklo, M AF Picklo, Matthew TI Supplementation with vitamin E and vitamin C inversely alters mitochondrial copy number and mitochondrial protein in obese, exercising rats SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Picklo, Matthew] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1030.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000208 ER PT J AU Raatz, S Beals, K Johnson, L Picklo, M AF Raatz, Susan Beals, Katherine Johnson, LuAnn Picklo, Matthew TI Chronic intake of honey, sugar and high fructose corn syrup exert equivalent effects on glucose and insulin SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Raatz, Susan] Univ Minnesota, St Paul, MN 55108 USA. [Beals, Katherine] Univ Utah, Salt Lake City, UT USA. [Raatz, Susan; Johnson, LuAnn; Picklo, Matthew] ARS, Grand Forks Human Nutr Res Ctr, USDA, Grand Forks, ND USA. NR 0 TC 0 Z9 0 U1 5 U2 9 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1039.4 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000260 ER PT J AU Reed, S Qin, X Ran-Ressler, R Brenna, J Glahn, R Tako, E AF Reed, Spenser Qin, Xia Ran-Ressler, Rinat Brenna, J. Glahn, Raymond Tako, Elad TI Dietary zinc deficiency affects blood linoleic acid: dihomo-gamma-linolenic acid ratio: a sensitive physiological marker of zinc status in vivo (Gallus gallus) SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Qin, Xia] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Reed, Spenser; Ran-Ressler, Rinat; Brenna, J.] Cornell Univ, Div Nutr Sci, Ithaca, NY 14853 USA. [Glahn, Raymond; Tako, Elad] Cornell Univ, USDA ARS, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. NR 0 TC 0 Z9 0 U1 0 U2 2 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1043.2 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000292 ER PT J AU Shi, XM Wen, SX Chacko, S Chan, L Guan, XF AF Shi, Xuemei Wen, Shixiang Chacko, Shaji Chan, Lawrence Guan, Xinfu TI Glp2r is required for postprandial glycemic control SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Chan, Lawrence] Baylor Coll Med, Div Diabet Endocrinol & Metab, Houston, TX 77030 USA. [Shi, Xuemei; Wen, Shixiang; Chacko, Shaji; Guan, Xinfu] Baylor Coll Med, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1114.3 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651001362 ER PT J AU Tako, E Cherian, B Reed, S Budiman, J Glahn, R AF Tako, Elad Cherian, Binu Reed, Spenser Budiman, Jessica Glahn, Raymond TI Biofortified pearl millet (Pennisetum glaucum L. provides more bioavailable iron than standard pearl millet: studies in poultry (Gallus gallus) and an in vitro digestion/Caco-2 model SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Reed, Spenser; Budiman, Jessica] Cornell Univ, Ithaca, NY USA. [Cherian, Binu] Int Food Policy Res Inst, Washington, DC 20036 USA. [Tako, Elad; Glahn, Raymond] Cornell Univ, USDA ARS, Robert Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1042.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000282 ER PT J AU Turner, J Feng, R Donnelly, J Schumacher, M Zavros, Y AF Turner, Jacob Feng, Rui Donnelly, Jessica Schumacher, Michael Zavros, Yana TI Mesenchymal stem cells isolated from the bone marrow of mice with gastritis induce epithelial-mesenchymal transition of gastric organoids SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Turner, Jacob; Feng, Rui; Schumacher, Michael; Zavros, Yana] Univ Cincinnati, Cincinnati, OH USA. [Donnelly, Jessica] ARS, USDA, Childrens Nutr Res Ctr, Houston, TX USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1119.5 PG 1 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651001388 ER PT J AU Wang, H Troy, L Fox, C Meigs, J McKeown, N Jacques, P AF Wang, Huifen Troy, Lisa Fox, Caroline Meigs, James McKeown, Nicola Jacques, Paul TI Longitudinal association of dairy consumption with changes in blood pressure and incident hypertension SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Wang, Huifen; McKeown, Nicola; Jacques, Paul] Tufts Univ, USDA, Human Nutr Res Ctr Aging, JM, Boston, MA 02111 USA. [Meigs, James] Massachusetts Gen Hosp, Boston, MA 02114 USA. [Fox, Caroline] NHLBI Framingham Heart Study, Framingham, MA USA. [Troy, Lisa] Univ Massachusetts, Amherst, MA 01003 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1018.3 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000113 ER PT J AU Wang, HF Jacques, P AF Wang, Huifen Jacques, Paul TI Dairy consumption among American children and adults: National Health and Nutrition Examination Survey 1999-2008 SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Wang, Huifen; Jacques, Paul] Tufts Univ, JM USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1018.1 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000109 ER PT J AU Xiong, JJ Must, A Wang, XD Gu, ZY Xu, WY Zhang, FF AF Xiong, Jianjing Must, Aviva Wang, Xiangdong Gu, Zhengyi Xu, Wenyan Zhang, Fangfang TI Predictors of overweight or obesity in school age children in Shanghai, China SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Xiong, Jianjing; Zhang, Fangfang] Tufts Univ, Friedman Sch Nutr Sci & Policy, Dept Nutr Sci, Boston, MA 02111 USA. [Must, Aviva] Tufts Univ, Dept Publ Hlth & Community Med, Boston, MA 02111 USA. [Zhang, Fangfang] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Xiong, Jianjing; Wang, Xiangdong; Gu, Zhengyi; Xu, Wenyan] Zhabei Dist Ctr Dis Control & Prevent, Shanghai, Peoples R China. NR 0 TC 0 Z9 0 U1 1 U2 3 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1024.4 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000163 ER PT J AU Zerofsky, M Jacoby, B Stephensen, C AF Zerofsky, Melissa Jacoby, Bryon Stephensen, Charles TI A randomized controlled trial of vitamin D supplementation in pregnancy: effects on vitamin D status and clinical outcomes SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Stephensen, Charles] ARS, USDA, WHNRC, Davis, CA USA. [Zerofsky, Melissa] Univ Calif Davis, Davis, CA 95616 USA. [Jacoby, Bryon] Univ Calif Davis, Div Maternal Fetal Med, Sch Med, Sacramento, CA 95817 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1041.5 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000277 ER PT J AU Zhang, FF Saltzman, E Must, A Liu, S Kelly, M Parsons, S Roberts, S AF Zhang, Fang Fang Saltzman, Edward Must, Aviva Liu, Shanshan Kelly, Michael Parsons, Susan Roberts, Susan TI Comparison of childhood cancer survivors' dietary intake with US dietary guidelines SO FASEB JOURNAL LA English DT Meeting Abstract C1 [Parsons, Susan] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies 4, Boston, MA USA. [Saltzman, Edward; Roberts, Susan] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Zhang, Fang Fang; Must, Aviva; Liu, Shanshan; Kelly, Michael] Tufts Univ, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 1 U2 1 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 1 SU S MA 1024.2 PG 2 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AX0OG UT WOS:000346651000162 ER PT J AU Moore, CE Radcliffe, JD Liu, Y AF Moore, Carolyn E. Radcliffe, John D. Liu, Yan TI Vitamin D intakes of adults differ by income, gender and race/ethnicity in the USA, 2007 to 2010 SO PUBLIC HEALTH NUTRITION LA English DT Article DE Vitamin D; Income; Ethnicity; Gender ID 25-HYDROXYVITAMIN D CONCENTRATIONS; HEALTH DISPARITIES; UNITED-STATES; D DEFICIENCY; WHITE WOMEN; SUPPLEMENTS; AMERICAN; FOODS; FORTIFICATION; PREVALENCE AB Objective: To determine if dietary, supplemental and total vitamin D intakes in the USA are influenced by income, race/ethnicity or gender. Design: Cross-sectional. US vitamin D intakes were estimated by poverty income ratio (PIR), race/ethnicity and gender using 24 h dietary intake recalls and dietary supplement use questionnaires. Statistical analyses of weighted data were performed using SAS (version 9? 2) to estimate means and their standard errors. Race and ethnic intake differences controlling for PIR, gender and age were assessed by ANCOVA. Subjects: Adults aged >= 19 years. Setting: The 2007-2010 National Health and Nutrition Examination Survey, USA. Results: Total (dietary and supplement) vitamin D intake was greater in the high (10.0 (SE 0.30) mu g/d) nu. the medium (7.9 (SE 0.3) mu g/d) or the low (8.0 (SE 0.3) mu g/d) PIR categories. Total vitamin D intake of non-Hispanic Whites (10.6 (SE 0.4) mu g/d) was greater than that of Hispanics (8.1 (SE 0.3) mu g/d) and non-Hispanic Blacks (7.1 (SE 0.3) mu g/d). Supplemental vitamin D intake was greater by females (5.3 (SE 0.2) mu g/d) than by males (3.3 (SE 0.2) mu g/d). Participants with high income were more likely to be vitamin D supplement users (33.0%) than those with medium (22.5%) or low (17.6%) income. High-income non-Hispanic Whites had the lowest percentage (57 %) not meeting the Estimated Average Requirement for vitamin D. Fortified milk and milk products provided 43.7% of the dietary vitamin D intake. Conclusions: Public health efforts should expand the number of vitamin D-fortified foods and encourage the consumption of foods high in vitamin D and use of supplements. C1 [Moore, Carolyn E.; Radcliffe, John D.] Texas Womans Univ, Dept Nutr & Food Sci, Houston, TX 77030 USA. [Liu, Yan] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. RP Moore, CE (reprint author), Texas Womans Univ, Dept Nutr & Food Sci, 6700 Fannin, Houston, TX 77030 USA. EM cmoore8@twu.edu FU Texas Woman's University Office of Research & Sponsored Programs FX Sources of funding: This project was funded by a Chancellor's Research Fellowship from Texas Woman's University Office of Research & Sponsored Programs. The Texas Woman's University Office of Research & Sponsored Programs had no role in the design, analysis or writing of this article. NR 44 TC 7 Z9 8 U1 1 U2 4 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD APR PY 2014 VL 17 IS 4 BP 756 EP 763 DI 10.1017/S1368980013002929 PG 8 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA AW6TX UT WOS:000346402200006 PM 24176054 ER PT J AU Zoellner, J Thomson, JL Landry, AS Anderson-Lewis, C Connell, C Molaison, EF Yadrick, K AF Zoellner, Jamie Thomson, Jessica L. Landry, Alicia S. Anderson-Lewis, Charkarra Connell, Carol Molaison, Elaine Fontenot Yadrick, Kathleen TI Improvements in Blood Pressure Among Undiagnosed Hypertensive Participants in a Community-Based Lifestyle Intervention, Mississippi, 2010 SO PREVENTING CHRONIC DISEASE LA English DT Article ID AMERICAN-HEART-ASSOCIATION; UNCONTROLLED HYPERTENSION; PHYSICAL-ACTIVITY; CLINICAL-TRIAL; UNITED-STATES; REDUCTION; NUTRITION; DISEASE; ADULTS; MODEL AB Introduction Effective strategies are needed to reach and treat people who lack awareness of or have uncontrolled hypertension. We used data from a community-based participatory research initiative, Hub City Steps, to quantify the prevalence of undiagnosed hypertension and determine the relationship between hypertension status at baseline and postintervention improvements in blood pressure and health-related quality of life. Methods Hub City Steps was a 6-month preintervention postintervention lifestyle intervention targeting hypertension risk factors. Outcome measures were collected at baseline, 3 months, and 6 months. Generalized linear mixed models were used to test for effects by time and hypertension status. Results Of the enrolled sample (N = 269), most were overweight or obese (91%), African American (94%), and women (85%). When considering hypertension status, 42% had self-reported diagnosis of hypertension (self-reported subgroup; 84% with antihypertensive medication use); 36% had no self-reported medical history of hypertension, but when blood pressure was measured they had a clinical diagnosis of prehypertension or hypertension (undiagnosed subgroup); and 22% had no self-reported or clinical hypertension diagnosis (no hypertension subgroup). From baseline to 6 months, systolic blood pressure significantly improved for participants with self-reported hypertension [8.2 (SD, 18.2) mm Hg] and undiagnosed hypertension [12.3 (SD, 16 3) mm Ha with undiagnosed participants experiencing the greatest improvements (P < .001). Effects remained significant after controlling for covariates. Health-related quality of life significantly improved for all 3 hypertension subgroups, with no apparent subgroup differences. Conclusion This study reveals advantages of a culturally appropriate community-based participatory research initiative to reach those with undetected hypertension and effectively improve blood pressure status and health-related quality of life. C1 [Zoellner, Jamie] Virginia Tech, Dept Human Nutr Foods & Exercise, Blacksburg, VA 24061 USA. [Thomson, Jessica L.] ARS, USDA, Stoneville, MS USA. [Landry, Alicia S.; Connell, Carol; Molaison, Elaine Fontenot; Yadrick, Kathleen] Univ So Mississippi, Hattiesburg, MS 39406 USA. [Anderson-Lewis, Charkarra] Univ Florida, Gainesville, FL USA. RP Zoellner, J (reprint author), Virginia Tech, Dept Human Nutr Foods & Exercise, Integrated Life Sci Bldg 23,Room 1034,0913, Blacksburg, VA 24061 USA. EM zoellner@vt.edu NR 30 TC 2 Z9 2 U1 1 U2 4 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD APR PY 2014 VL 11 AR 130269 DI 10.5888/pcd11.130269 PG 10 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AR3XG UT WOS:000343521900011 ER PT J AU Rodriguez-Vivas, RI Perez-Cogollo, LC Rosado-Aguilar, JA Ojeda-Chi, MM Trinidad-Martinez, I Miller, RJ Li, AY de Leon, AP Guerrero, F Klafke, G AF Ivan Rodriguez-Vivas, Roger Carlos Perez-Cogollo, Luis Alberto Rosado-Aguilar, Jose Maribel Ojeda-Chi, Melina Trinidad-Martinez, Iris Miller, Robert John Li, Andrew Yongsheng de Leon, Adalberto Perez Guerrero, Felix Klafke, Guilherme TI Rhipicephalus (Boophilus) microplus resistant to acaricides and ivermectin in cattle farms of Mexico SO REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA LA English DT Review DE Rhipicephalus (B.) microplus; acaricide; resistance; macrocyclic lactone; tick control ID GATED CHLORIDE CHANNEL; POTENTIAL RISK-FACTORS; IN-VIVO EVALUATION; MACROCYCLIC LACTONES; FORMAMIDINE PESTICIDES; PYRETHROID RESISTANCE; OCTOPAMINE RECEPTORS; PERSISTENT EFFICACY; AMITRAZ RESISTANCE; MANAGEMENT FACTORS AB Ticks and the diseases they transmit cause great economic losses to livestock in tropical countries. Non-chemical control alternatives include the use of resistant cattle breeds, biological control and vaccines. However, the most widely used method is the application of different chemical classes of acaricides and macrocyclic lactones. Populations of the cattle tick, Rhipicephalus (Boophilus) microplus, resistant to organophosphates (OP), synthetic pyrethroids (SP), amitraz and fipronil have been reported in Mexico. Macrocyclic lactones are the most sold antiparasitic drug in the Mexican veterinary market. Ivermectin-resistant populations of R. (B.) microplus have been reported in Brazil, Uruguay and especially in Mexico (Veracruz and Yucatan). Although ivermectin resistance levels in R. (B.) microplus from Mexico were generally low in most cases, some field populations of R. (B.) microplus exhibited high levels of ivermectin resistance. The CHPAT population showed a resistance ratio of 10.23 and 79.6 at lethal concentration of 50% and 99%, respectively. Many field populations of R. (B.) microplus are resistant to multiple classes of antiparasitic drugs, including organophosphates (chlorpyrifos, coumaphos and diazinon), pyrethroids (flumethrin, deltamethrin and cypermethrin), amitraz and ivermectin. This paper reports the current status of the resistance of R. (B.) microplus to acaricides, especially ivermectin, in Mexican cattle. C1 [Ivan Rodriguez-Vivas, Roger; Carlos Perez-Cogollo, Luis; Alberto Rosado-Aguilar, Jose; Maribel Ojeda-Chi, Melina; Trinidad-Martinez, Iris] Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Merida 97100, Yucatan, Mexico. [Miller, Robert John] USDA, Cattle Fever Tick Res Lab, Edinburg, TX USA. [Li, Andrew Yongsheng; de Leon, Adalberto Perez; Guerrero, Felix] USDA ARS, Knipling Bushland US Livestock Insects Res La, Kerrville, TX 78029 USA. [Klafke, Guilherme] FEPAGRO Saude Anim, Parasitol Lab, Eldorado Do Sul, RS, Brazil. RP Rodriguez-Vivas, RI (reprint author), Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Km 15-5 Carretera Merida Xmatkuil, Merida 97100, Yucatan, Mexico. EM rvivas@uady.mx NR 85 TC 8 Z9 8 U1 3 U2 16 PU BRAZILIAN COLL VETERINARY PARASITOLOGY PI SAO PAULO PA C/O ASSISTANT EDITOR AV PROF LINEU PRESTES, 1374, CIDADE UNIVERSITARIA, CEP 05508-900, SAO PAULO, 00000, BRAZIL SN 1984-2961 J9 REV BRAS PARASITOL V JI Rev. Bras. Parasitol. Vet. PD APR-JUN PY 2014 VL 23 IS 2 BP 113 EP 122 DI 10.1590/S1984-29612014044 PG 10 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AQ7IT UT WOS:000342986900001 PM 25054487 ER PT J AU Grisi, L Leite, RC Martins, JRD de Barros, ATM Andreotti, R Cancado, PHD de Leon, AAP Pereira, JB Villela, HS AF Grisi, Laerte Leite, Romario Cerqueira de Souza Martins, Joao Ricardo Medeiros de Barros, Antonio Thadeu Andreotti, Renato Duarte Cancado, Paulo Henrique Perez de Leon, Adalberto Angel Pereira, Jairo Barros Villela, Humberto Silva TI Reassessment of the potential economic impact of cattle parasites in Brazil SO REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA LA English DT Article DE Cattle parasites; production loss; economic assessment ID RHIPICEPHALUS BOOPHILUS MICROPLUS; MILK-PRODUCTION; DAIRY-CATTLE; HAEMATOBIA-IRRITANS; DERMATOBIA-HOMINIS; BEEF-CATTLE; MATO-GROSSO; NELLORE; RESISTANCE; STATE AB The profitability of livestock activities can be diminished significantly by the effects of parasites. Economic losses caused by cattle parasites in Brazil were estimated on an annual basis, considering the total number of animals at risk and the potential detrimental effects of parasitism on cattle productivity. Estimates in U. S. dollars (USD) were based on reported yield losses among untreated animals and reflected some of the effects of parasitic diseases. Relevant parasites that affect cattle productivity in Brazil, and their economic impact in USD billions include: gastrointestinal nematodes - $ 7.11; cattle tick (Rhipicephalus (Boophilus) microplus) - $ 3.24; horn fly (Haematobia irritans) - $ 2.56; cattle grub (Dermatobia hominis) - $0.38; New World screwworm fly (Cochliomyia hominivorax) - $ 0.34; and stable fly (Stomoxys calcitrans) - $ 0.34. The combined annual economic loss due to internal and external parasites of cattle in Brazil considered here was estimated to be at least USD 13.96 billion. These findings are discussed in the context of methodologies and research that are required in order to improve the accuracy of these economic impact assessments. This information needs to be taken into consideration when developing sustainable policies for mitigating the impact of parasitism on the profitability of Brazilian cattle producers. C1 [Grisi, Laerte] UFRRJ, BR-23890000 Seropedica, RJ, Brazil. [Leite, Romario Cerqueira] Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil. [de Souza Martins, Joao Ricardo] FEPAGRO, Inst Pesquisas Vet Desidrio Finamor, Eldorado Do Sul, RS, Brazil. [Medeiros de Barros, Antonio Thadeu; Andreotti, Renato; Duarte Cancado, Paulo Henrique] Embrapa Gado de Corte, Campo Grande, MS, Brazil. [Perez de Leon, Adalberto Angel] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78029 USA. [Pereira, Jairo Barros; Villela, Humberto Silva] Champ Farmoquim Ltda, Anapolis, Go, Brazil. RP Grisi, L (reprint author), UFRRJ, Ins Vet, Dept Parasitol Anim, BR 465,Km 7, BR-23890000 Seropedica, RJ, Brazil. EM lgrisi@ufrrj.br NR 43 TC 53 Z9 54 U1 4 U2 35 PU BRAZILIAN COLL VETERINARY PARASITOLOGY PI SAO PAULO PA C/O ASSISTANT EDITOR AV PROF LINEU PRESTES, 1374, CIDADE UNIVERSITARIA, CEP 05508-900, SAO PAULO, 00000, BRAZIL SN 1984-2961 J9 REV BRAS PARASITOL V JI Rev. Bras. Parasitol. Vet. PD APR-JUN PY 2014 VL 23 IS 2 BP 150 EP 156 DI 10.1590/S1984-29612014042 PG 7 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AQ7IT UT WOS:000342986900006 PM 25054492 ER PT J AU Slater, MA Anderson, RC AF Slater, Mitchell A. Anderson, Roger C. TI Intensive Selective Deer Browsing Favors Success of Asimina triloba (Paw paw) a Native Tree Species SO NATURAL AREAS JOURNAL LA English DT Article DE Acer saccharum; Asimina triloba; deer herbivory; Odocoileus virginianus ID WHITE-TAILED DEER; CENTRAL ILLINOIS FORESTS; ALTERNATE STABLE STATES; EASTERN UNITED-STATES; SOUTHWESTERN ILLINOIS; UNDERSTORY TREE; HARDWOOD FOREST; ACER-SACCHARUM; REGENERATION; CANOPY AB Although white-tailed deer (Odocoileus virginianus Zimmermann) are generalist herbivores, they can have significant effects on species composition and abundance of forest trees, especially when deer densities are high and most plant species are heavily browsed but a few are selectively avoided as browse. We evaluated effects of selective deer browsing on tree species abundance in an old-growth mesic/wet-mesic forest in central Illinois by repeated sampling of permanent study plots in 2003 and 2008 and relating changes in stem density between the two samples to the intensity of deer browsing of individual species. The study site has high deer density (75 deer km(2)) during winter months, and initial observations indicated that paw paw (Asimina triloba (L.) Dunal) was strongly avoided as browse. Paw paw density increased in the seedling stratum between the two sample periods. However, nearly all other tree species declined in density; blue ash (Fraxinus quadrangulata Michx.) - a low-use browse species, had a small increase in seedling density. Tree species diversity decreased in the seedling stratum, but not in the sapling stratum, although sapling density declined for some species. The current trajectory of tree species growth and recruitment suggests that the development of a dense paw paw understory canopy will further decrease species diversity and reduce tree species recruitment. Based on the life history traits of paw paw, and high deer densities and selective browsing, this issue is of concern for current and future forest communities. C1 [Slater, Mitchell A.] Forest Serv, No Res Stn, USDA, Grand Rapids, MN 55744 USA. [Anderson, Roger C.] Illinois State Univ, Sch Biol Sci, Normal, IL 61790 USA. RP Slater, MA (reprint author), Forest Serv, No Res Stn, USDA, 1831 Highway 169, Grand Rapids, MN 55744 USA. EM maslater@fs.fed.us FU Sugar Grove Foundation; Beta Lambda Chapter of the Phi Sigma Biological Honor Society FX We thank the Sugar Grove Foundation for allowing us to establish our permanent research plots in Funks Grove and providing funding to support a graduate research assistant during the summer of 2003, and the Beta Lambda Chapter of the Phi Sigma Biological Honor Society, which provided partial funding for this project. We also thank Dr. Steven A. Juliano, Dr. Rachel M. Bowden, Dr. Brian J. Palik, and M. Rebecca Anderson, who provided us with helpful reviews of the manuscript. NR 63 TC 1 Z9 1 U1 6 U2 31 PU NATURAL AREAS ASSOC PI ROCKFORD PA 320 SOUTH THIRD ST, ROCKFORD, IL 61104 USA SN 0885-8608 EI 2162-4399 J9 NAT AREA J JI Nat. Areas J. PD APR PY 2014 VL 34 IS 2 BP 178 EP 187 DI 10.3375/043.034.0207 PG 10 WC Ecology; Forestry SC Environmental Sciences & Ecology; Forestry GA AQ5VD UT WOS:000342875800006 ER PT J AU Thompson, PC Rosenthal, BM Hare, MP AF Thompson, Peter C. Rosenthal, Benjamin M. Hare, Matthew P. TI MICROSATELLITE GENOTYPES REVEAL SOME LONG-DISTANCE GENE FLOW IN PERKINSUS MARINUS, A MAJOR PATHOGEN OF THE EASTERN OYSTER, CRASSOSTREA VIRGINICA (GMELIN) SO JOURNAL OF SHELLFISH RESEARCH LA English DT Article DE microsatellite; genetic structure; parasite; oyster; dispersal; gene flow; biogeography; evolution; Perkinsus marinus; Crassostrea virginica ID POPULATION-STRUCTURE; CHESAPEAKE BAY; PARASITE; RANGE; PROTOZOAN; INFECTIVITY; TEMPERATURE; VIABILITY; INFERENCE; ATLANTIC AB As the agent of Dermo disease, Perkinsus marinus causes significant mortality and reduced fecundity its eastern oyster host, Crassostrea virginica. Passive dispersal of P. marinus between hosts subjects parasite movements to control by water currents in estuarine systems, potentially limiting connectivity among parasite populations in different estuaries. Given recent evidence for sexual reproduction in P. marinus, estimates of gene flow among locations may provide insights into this parasite's epidemiology. In this study, 1,082 wild oysters were collected from 2002 to 2008 at 15 geographical locations encompassing 4,800 km of the Gulf of Mexico and Atlantic coasts of the United States. Of these, 742 oysters (68.9% prevalence) were determined to be infected by P. marinus. Among infected oysters, 374 were determined to be infected by a single parasite genotype based on amplification of seven microsatellite loci, and therefore were amenable to analysis. Allele frequencies differed significantly among most locales, but there was no indication of isolation by distance. Four distinct clusters of multilocus genotypes were identified by analyzing genetic distances among individuals and by using Bayesian assignment tests. One lineage occurred in many locations, whereas the others were limited to a particular region or disjunct locations. Two lineages were associated with recent range expansion. Altogether, local assemblages of P. marinus are characterized by mixtures of distinct sympatric populations that undergo only infrequent recombination. By mixing divergent strains, long-distance dispersal and/or anthropogenic introduction may play an important role in the evolution of P. marinus and spread of Dermo disease, whereas locally, high-frequency strains may represent focal epizootics. C1 [Thompson, Peter C.] Univ Maryland, Behav Ecol Evolut & Systemat Program, College Pk, MD 20742 USA. [Thompson, Peter C.; Rosenthal, Benjamin M.] ARS, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. [Hare, Matthew P.] Univ Maryland, Dept Biol, College Pk, MD 20742 USA. RP Thompson, PC (reprint author), Univ Maryland, Behav Ecol Evolut & Systemat Program, 2101 BioSci Res Bldg, College Pk, MD 20742 USA. EM pete.c.thompson@gmail.com OI Rosenthal, Benjamin/0000-0002-0224-3773 FU University of Maryland FX This work would not have been possible without the support of numerous state agencies that assisted in collecting samples. Wolf Pecher and Gerardo Vasta generously shared samples collected in the northeastern United States. This work was funded by a startup grant at the University of Maryland and salary support to P. Thompson from the U.S. Department of Agriculture Agricultural Research Service. NR 43 TC 4 Z9 4 U1 3 U2 9 PU NATL SHELLFISHERIES ASSOC PI GROTON PA C/O DR. SANDRA E. SHUMWAY, UNIV CONNECTICUT, 1080 SHENNECOSSETT RD, GROTON, CT 06340 USA SN 0730-8000 EI 1943-6319 J9 J SHELLFISH RES JI J. Shellfish Res. PD APR PY 2014 VL 33 IS 1 BP 195 EP 206 DI 10.2983/035.033.0119 PG 12 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AQ3KX UT WOS:000342693300019 ER PT J AU Morris, JB Wang, ML Tonnis, B AF Morris, J. B. Wang, M. L. Tonnis, B. TI Desmodium genetic resources for improving flavonoid concentrations, oil content and fatty acid compositions SO PLANT GENETIC RESOURCES-CHARACTERIZATION AND UTILIZATION LA English DT Article DE Flavonoids; oil; fatty acids; Desmodium ID CANCER CELLS; PERFORMANCE; APOPTOSIS; LEGUMES; PATHWAY; GROWTH AB Several Desmodium species are adapted to the environment of Griffin, Georgia, USA. The determination of flavonoid concentrations, oil content and fatty acid compositions of 25 Desmodium accessions representing five species (D. discolor Vogel, D. incanum (G. Mey.) DC., D. intortum (Mill.) Urb., D. sandwicense E. Mey. and D. tortuosum (Sw.) DC.) would add value to the members of the genus Desmodium for possible use as livestock health supplements. In this study, the seeds of these 25 accessions were evaluated for flavonoid concentrations, oil content and fatty acid compositions using high-performance liquid chromatography, nuclear magnetic resonance and gas chromatography, respectively. Several accessions exhibited significantly greater values for all the traits than the controls. The Desmodium accessions produced significantly greater concentrations of quercetin and kaempferol than the best control accession (D. incanum, PI 477072). However, all the Desmodium accessions produced significantly greater concentrations of isorhamnetin and luteolin than the control accessions. All the Desmodium accessions had greater linoleic (18:2) and behenic (22:0) acid content than a couple of D. incanum control accessions in 2010 and 2011. Significant correlations were observed between several traits. The concentration of quercetin was significantly correlated with that of kaempferol (r(2) = 0.69***); however, the concentration of quercetin exhibited a significantly negative correlation (r(2) = -0.41*) with that of isorhamnetin. Oil content was significantly correlated with palmitic acid (r(2) = 0.61**), stearic acid (r(2) = 0.81***), linolenic acid (r(2) = 0.58**) and lignoceric acid (r(2) = 0.80***) content. This information will assist breeders and other scientists in developing superior cultivars with optimum levels of flavonoid concentrations, oil content and fatty acid compositions for many of these Desmodium species. C1 [Morris, J. B.; Wang, M. L.; Tonnis, B.] USDA ARS, PGRCU, Griffin, GA 30223 USA. RP Morris, JB (reprint author), USDA ARS, PGRCU, 1109 Expt St, Griffin, GA 30223 USA. EM brad.morris@ars.usda.gov NR 25 TC 0 Z9 0 U1 1 U2 3 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1479-2621 EI 1479-263X J9 PLANT GENET RESOUR-C JI Plant Genet. Resour.-Charact. Util. PD APR PY 2014 VL 12 IS 1 BP 120 EP 128 DI 10.1017/S1479262113000397 PG 9 WC Plant Sciences; Genetics & Heredity SC Plant Sciences; Genetics & Heredity GA AQ3CJ UT WOS:000342666200013 ER PT J AU Weller, JI Cole, JB VanRaden, PM Wiggans, GR AF Weller, J. I. Cole, J. B. VanRaden, P. M. Wiggans, G. R. TI Application of the a posteriori granddaughter design to the Holstein genome SO ANIMAL LA English DT Article DE granddaughter design; genomic evaluation; haplotype; quantitative trait locus; Holstein cow ID QUANTITATIVE TRAIT LOCI; SATURATED GENETIC-MAP; LINKAGE DISEQUILIBRIUM; MILK-PRODUCTION; DAIRY-CATTLE; CONFIDENCE-INTERVALS; CONFORMATION TRAITS; MISSENSE MUTATION; QTL; LOCATION AB An a posteriori granddaughter design was applied to estimate quantitative trait loci genotypes of sires with many sons in the US Holstein population. The results of this analysis can be used to determine concordance between specific polymorphisms and segregating quantitative trait loci. Determination of the actual polymorphisms responsible for observed genetic variation should increase the accuracy of genomic evaluations and rates of genetic gain. A total of 52 grandsire families, each with >= 100 genotyped sons with genetic evaluations based on progeny tests, were analyzed for 33 traits (milk, fat and protein yields; fat and protein percentages; somatic cell score (SCS); productive life; daughter pregnancy rate; heifer and cow conception rates; service-sire and daughter calving ease; service-sire and daughter stillbirth rates; 18 conformation traits; and net merit). Of 617 haplotype segments spanning the entire bovine genome and each including similar to 5 x 10(6) bp, 5 cM and 50 genes, 608 autosomal segments were analyzed. A total of 19 335 unique haplotypes were found among the 52 grandsires. There were a total of 133 chromosomal segment-by-trait combinations, for which the nominal probability of significance for the haplotype effect was < 10(-8), which corresponds to genome-wide significance of < 10(-4). The number of chromosomal regions that met this criterion by trait ranged from one for rear legs (rear view) to seven for net merit. For each of the putative quantitative trait loci, at least one grandsire family had a within-family contrast with a t-value of > 3. Confidence intervals (CIs) were estimated by the nonparametric bootstrap for the largest effect for each of nine traits. The bootstrap distribution generated by 100 samples was bimodal only for net merit, which had the widest 90% CI (eight haplotype segments). This may be due to the fact that net merit is a composite trait. For all other chromosomes, the CI spanned less than a third of the chromosome. The narrowest CI (a single haplotype segment) was found for SCS. It is likely that analysis by more advanced methods could further reduce CIs at least by half. These results can be used as a first step to determine the actual polymorphisms responsible for observed quantitative variation in dairy cattle. C1 [Weller, J. I.] Agr Res Org, Volcani Ctr, Inst Anim Sci, IL-50250 Bet Dagan, Israel. [Cole, J. B.; VanRaden, P. M.; Wiggans, G. R.] ARS, USDA, Anim Improvement Programs Lab, Beltsville, MD 20705 USA. RP Weller, JI (reprint author), Agr Res Org, Volcani Ctr, Inst Anim Sci, IL-50250 Bet Dagan, Israel. EM weller@agri.huji.ac.il RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU Binational Agricultural Research and Development Fund [IS-4394-11R] FX This research was supported by a grant from the Binational Agricultural Research and Development Fund Research Project IS-4394-11R. The authors thank S. M. Hubbard, Animal Improvement Programs Laboratory, Agricultural Research Service, US Department of Agriculture (Beltsville, MD, USA), for technical manuscript review. NR 35 TC 0 Z9 0 U1 0 U2 2 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD APR PY 2014 VL 8 IS 4 BP 511 EP 519 DI 10.1017/S1751731114000111 PG 9 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AP6RT UT WOS:000342206000001 PM 24636822 ER PT J AU Atherly, T Ziemer, CJ AF Atherly, Todd Ziemer, Cherie J. TI Bacteroides isolated from four mammalian hosts lack host-specific 16S rRNA gene phylogeny and carbon and nitrogen utilization patterns SO MICROBIOLOGYOPEN LA English DT Article DE Bacteroides; cow; fecal bacteria; goat; gut microbiota; human; phenotype array; pig ID HUMAN INTESTINE; GUT MICROBIOTA; DIVERSITY; EVOLUTION; BACTERIA; THETAIOTAOMICRON; POLYSACCHARIDES; MUTUALISM; SYMBIONT; FRAGILIS AB One-hundred-and-three isolates of Bacteroides ovatus, B. thetaiotaomicron, and B. xylanisolvens were recovered from cow, goat, human, and pig fecal enrichments with cellulose or xylan/pectin. Isolates were compared using 16S rRNA gene sequencing, repetitive sequence-based polymerase chain reaction (repPCR), and phenotypic microarrays. Analysis of 16S rRNA gene sequences revealed high sequence identity in these Bacteroides; with distinct phylogenetic groupings by bacterial species but not host origin. Phenotypic microarray analysis demonstrated these Bacteroides shared the ability to utilize many of the same carbon substrates, without differences due to species or host origin, indicative of their broad carbohydrate fermentation abilities. Limited nitrogen substrates were utilized; in addition to ammonia, guanine, and xanthine, purine derivatives were utilized by most isolates followed by a few amino sugars. Only repPCR analysis demonstrated host-specific patterns, indicating that genomic changes due to coevolution with host did not occur by mutation in the 16S rRNA gene or by a gain or loss of carbohydrate utilization genes within these Bacteroides. This is the first report to indicate that host-associated genomic differences are outside of 16S rRNA gene and carbohydrate utilization genes and suggest conservation of specific bacterial species with the same functionality across mammalian hosts for this Bacteroidetes clade. C1 [Atherly, Todd; Ziemer, Cherie J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50010 USA. RP Ziemer, CJ (reprint author), USDA ARS MWA NLAE, 2110 Univ Blvd,2202 NSRIC, Ames, IA 50011 USA. EM cherie.ziemer@ars.usda.gov FU Defense Advanced Research Projects Agency; Intestinal Fortitude and Crystalline Cellulose Conversion to Glucose programs FX This research was supported by grants from Defense Advanced Research Projects Agency as part of the Intestinal Fortitude and Crystalline Cellulose Conversion to Glucose programs to C.J. Ziemer. NR 29 TC 2 Z9 2 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-8827 J9 MICROBIOLOGYOPEN JI MicrobiologyOpen PD APR PY 2014 VL 3 IS 2 BP 225 EP 238 DI 10.1002/mbo3.159 PG 14 WC Microbiology SC Microbiology GA AP8TQ UT WOS:000342352400006 PM 24532571 ER PT J AU Svendsen, ES Baine, G Northridge, ME Campbell, LK Metcalf, SS AF Svendsen, Erika S. Baine, Gillian Northridge, Mary E. Campbell, Lindsay K. Metcalf, Sara S. TI Recognizing Resilience SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Editorial Material ID NEW-YORK-CITY; ENVIRONMENTAL STEWARDSHIP; CRIME C1 [Svendsen, Erika S.; Baine, Gillian; Campbell, Lindsay K.] US Forest Serv, USDA, No Res Stn, New York City Urban Field Stn, New York, NY 10007 USA. [Northridge, Mary E.] NYU, Coll Dent, Dept Epidemiol & Hlth Promot, New York, NY USA. [Metcalf, Sara S.] SUNY Buffalo, Dept Geog, Buffalo, NY 14260 USA. RP Svendsen, ES (reprint author), US Forest Serv, USDA, No Res Stn, 290 Broadway,26th Floor, New York, NY 10007 USA. EM esvendsen@fs.fed.us NR 12 TC 6 Z9 6 U1 2 U2 7 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD APR PY 2014 VL 104 IS 4 BP 581 EP 583 DI 10.2105/AJPH.2013.301848 PG 3 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AP0XI UT WOS:000341789200023 PM 24524524 ER PT J AU Uejio, CK Yale, SH Malecki, K Borchardt, MA Anderson, HA Patz, JA AF Uejio, Christopher K. Yale, Steven H. Malecki, Kristen Borchardt, Mark A. Anderson, Henry A. Patz, Jonathan A. TI Drinking Water Systems, Hydrology, and Childhood Gastrointestinal Illness in Central and Northern Wisconsin SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID EMERGENCY-DEPARTMENT VISITS; HUMAN ENTERIC VIRUSES; UNITED-STATES; TIME-SERIES; DISEASE OUTBREAKS; DIARRHEAL ILLNESS; CLIMATE-CHANGE; HEALTH-CARE; SURVEILLANCE; SEASONALITY AB Objectives. This study investigated if the type of drinking water source (treated municipal, untreated municipal, and private well water) modifies the effect of hydrology on childhood (aged < 5 years) gastrointestinal illness. Methods. We conducted a time series study to assess the relationship between hydrologic and weather conditions with childhood gastrointestinal illness from 1991 to 2010. The Central and Northern Wisconsin study area includes households using all 3 types of drinking water systems. Separate time series models were created for each system and half-year period (winter/spring, summer/fall). Results. More precipitation (summer/fall) systematically increased childhood gastrointestinal illness in municipalities accessing untreated water. The relative risk of contracting gastrointestinal illness was 1.4 in weeks with 3 centimeters of precipitation and 2.4 in very wet weeks with 12 centimeters of precipitation. By contrast, gastrointestinal illness in private well and treated municipal areas was not influenced by hydrologic conditions, although warmer winter temperatures slightly increased incidence. Conclusions. Our study suggests that improved drinking water protection, treatment, and delivery infrastructure may improve public health by specifically identifying municipal water systems lacking water treatment that may transmit waterborne disease. C1 [Uejio, Christopher K.] Florida State Univ, Dept Geog, Tallahassee, FL 32306 USA. [Uejio, Christopher K.] Florida State Univ, Program Publ Hlth, Tallahassee, FL 32306 USA. [Yale, Steven H.] Marshfield Clin Res Fdn, Clin Res Ctr, Marshfield, WI USA. [Malecki, Kristen] Univ Wisconsin, Dept Populat Hlth Sci, Madison, WI 53706 USA. [Borchardt, Mark A.] USDA ARS, Marshfield, WI USA. [Anderson, Henry A.] Wisconsin Dept Hlth Serv, Madison, WI USA. [Patz, Jonathan A.] Univ Wisconsin, Nelson Inst Ctr Sustainabil & Global Environm, Madison, WI 53706 USA. RP Uejio, CK (reprint author), Florida State Univ, Dept Geog, Room 323,Bellamy Bldg,113 Collegiate Loop, Tallahassee, FL 32306 USA. EM cuejio@fsu.edu FU Centers for Disease Control and Prevention [1U01EH000428-01]; National Center for Atmospheric Research/Centers for Disease Control and Prevention Fellowship Program; National PERISHIP Dissertation Fellowship - National Science Foundation; University of Colorado Natural Hazards Center; Swiss Re; Public Entity Risk Institute FX This work was supported by the Centers for Disease Control and Prevention (grant 1U01EH000428-01), the National Center for Atmospheric Research/Centers for Disease Control and Prevention Fellowship Program, and the National PERISHIP Dissertation Fellowship funded by The National Science Foundation, University of Colorado Natural Hazards Center, Swiss Re, and the Public Entity Risk Institute. NR 64 TC 5 Z9 5 U1 2 U2 16 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD APR PY 2014 VL 104 IS 4 BP 639 EP 646 DI 10.2105/AJPH.2013.301659 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AP0XI UT WOS:000341789200033 PM 24524509 ER PT J AU Anekwe, TD Rahkovsky, I AF Anekwe, Tobenna D. Rahkovsky, Ilya TI The Association Between Food Prices and the Blood Glucose Level of US Adults With Type 2 Diabetes SO AMERICAN JOURNAL OF PUBLIC HEALTH LA English DT Article ID TAXES; DIET; CONSUMPTION; VEGETABLES; WEIGHT; FRUITS; HEALTH; NUTS AB Objectives. We estimated the association between the price of healthy and less-healthy food groups and blood sugar among US adults with type 2 diabetes. Methods. We linked 1999-2006 National Health and Nutrition Examination Survey health information to food prices contained in the Quarterly Food-at-Home Price Database. We regressed blood sugar levels on food prices from the previous calendar quarter, controlling for market region and a range of other covariates. We also examined whether the association between food prices and blood sugar varies among different income groups. Results. The prices of produce and low-fat dairy foods were associated with blood sugar levels of people with type 2 diabetes. Specifically, higher prices for produce and low-fat dairy foods were associated with higher levels of glycated hemoglobin and fasting plasma glucose 3 months later. Food prices had a greater association with blood sugar for low-income people than for higher-income people, and in the expected direction. Conclusions. Higher prices of healthy foods were associated with increased blood sugar among people with type 2 diabetes. The association was especially pronounced among low-income people with type 2 diabetes. C1 [Anekwe, Tobenna D.; Rahkovsky, Ilya] USDA, Econ Res Serv, Washington, DC 20250 USA. RP Anekwe, TD (reprint author), USDA, Econ Res Serv, 1400 Independence Ave SW, Washington, DC 20250 USA. EM tanekwe@ers.usda.gov NR 38 TC 4 Z9 4 U1 0 U2 6 PU AMER PUBLIC HEALTH ASSOC INC PI WASHINGTON PA 800 I STREET, NW, WASHINGTON, DC 20001-3710 USA SN 0090-0036 EI 1541-0048 J9 AM J PUBLIC HEALTH JI Am. J. Public Health PD APR PY 2014 VL 104 IS 4 BP 678 EP 685 DI 10.2105/AJPH.2013.301661 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AP0XI UT WOS:000341789200038 PM 24524504 ER PT J AU Kulesza, SB Maguire, RO Thomason, WE Hodges, SC Pote, DH AF Kulesza, Stephanie B. Maguire, Rory O. Thomason, Wade E. Hodges, Steven C. Pote, Dan H. TI Effects of Poultry Litter Injection on Ammonia Volatilization, Nitrogen Availability, and Nutrient Losses in Runoff SO SOIL SCIENCE LA English DT Article DE Injection; poultry litter; volatilization; N mineralization; runoff ID NO-TILL; SUBSURFACE APPLICATION; FORAGE SYSTEMS; SOILS; PASTURE; EUTROPHICATION; PHOSPHORUS; QUALITY; NITRATE; WATER AB Poultry litter is a common organic amendment in agricultural production, but nutrient losses can reduce its effectiveness as a fertilizer. Three experiments were conducted to evaluate ammonia nitrogen (NH3-N) volatilization, N availability, and runoff losses of nutrients by conducting a closed chamber volatilization study, a soil incubation, and a rainfall simulation. In all studies, poultry litter was applied at a rate of 6.7 Mg ha(-1) either on the surface or injected and compared with an unamended control. In the volatilization and soil incubation studies, Braddock Loam and Bojac Sandy Loam surface soils were compared. Of the ammonium N added, cumulative loss of NH3-N by volatilization was 3% from injected and 121% from surface applied poultry litter after 7 days in the Loam. In the Sandy Loam, cumulative loss of NH3-N was 9% from injected and 153% from surface applied poultry litter after 7 days. After a 40-day soil incubation, injection increased total inorganic N by 52% and 99% for the Loam and Sandy Loam soils, respectively, when compared with surface application. Injection reduced total Kjeldahl N by 59%, total Kjeldahl P by 53%, dissolved reactive P, dissolved nitrate N by 73%, and dissolved NH3-N in runoff by 99%, compared with surface application. Injection reduced NH3-N volatilization and nutrients in runoff to levels of the control. These studies show that injection increases plant available N while decreasing losses through volatilization and runoff. C1 [Kulesza, Stephanie B.; Maguire, Rory O.; Thomason, Wade E.; Hodges, Steven C.] Virginia Tech, Dept Crop & Soil Environm Sci, Blacksburg, VA 24061 USA. [Pote, Dan H.] ARS, USDA, Bumpers Small Farms Res Ctr, Booneville, AR USA. RP Kulesza, SB (reprint author), Virginia Tech, Dept Crop & Soil Environm Sci, 424 Smyth Hall,185 Ag Quad Lane, Blacksburg, VA 24061 USA. EM skulesz@vt.edu NR 41 TC 2 Z9 2 U1 3 U2 19 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD APR PY 2014 VL 179 IS 4 BP 190 EP 196 DI 10.1097/SS.0000000000000058 PG 7 WC Soil Science SC Agriculture GA AP4TT UT WOS:000342072400003 ER PT J AU Nyaku, ST Sripathi, VR Kantety, RV Cseke, SB Buyyarapu, R Mc Ewan, R Gu, YQ Lawrence, K Senwo, Z Sripathi, P George, P Sharma, GC AF Nyaku, Seloame T. Sripathi, Venkateswara R. Kantety, Ramesh V. Cseke, Sarah B. Buyyarapu, Ramesh Mc Ewan, Robert Gu, Yong Q. Lawrence, Kathy Senwo, Zachary Sripathi, Padmini George, Pheba Sharma, Govind C. TI Characterization of the reniform nematode genome by shotgun sequencing SO GENOME LA English DT Article DE reniform nematode; detoxification genes; nematode parasitism genes; 454 sequencing ID PLANT-PARASITIC NEMATODE; LOW-COMPLEXITY REGIONS; ROOT-KNOT NEMATODE; BETA-1,4-ENDOGLUCANASE GENES; CAENORHABDITIS-ELEGANS; HETERODERA-GLYCINES; CYST NEMATODES; ROTYLENCHULUS-RENIFORMIS; MELOIDOGYNE-INCOGNITA; HYDROGEN-PEROXIDE AB The reniform nematode (RN), a major agricultural pest particularly on cotton in the United States, is among the major plant-parasitic nematodes for which limited genomic information exists. In this study, over 380 Mb of sequence data were generated from pooled DNA of four adult female RNs and assembled into 67 317 contigs, including 25 904 (38.5%) predicted coding contigs and 41 413 (61.5%) noncoding contigs. Most of the characterized repeats were of low complexity (88.9%), and 0.9% of the contigs matched with 53.2% of GenBank ESTs. The most frequent Gene Ontology (GO) terms for molecular function and biological process were protein binding (32%) and embryonic development (20%). Further analysis showed that 741 (1.1%), 94 (0.1%), and 169 (0.25%) RN genomic contigs matched with 1328 (13.9%), 1480 (5.4%), and 1330 (7.4%) supercontigs of Meloidogyne incognita, Brugia malayi, and Pristionchus pacificus, respectively. Chromosome 5 of Caenorhabditis elegans had the highest number of hits to the RN contigs. Seven putative detoxification genes and three carbohydrate-active enzymes (CAZymes) involved in cell wall degradation were studied in more detail. Additionally, kinases, G protein-coupled receptors, and neuropeptides functioning in physiological, developmental, and regulatory processes were identified in the RN genome. C1 [Nyaku, Seloame T.; Sripathi, Venkateswara R.; Kantety, Ramesh V.; Cseke, Sarah B.; Senwo, Zachary; Sripathi, Padmini; George, Pheba; Sharma, Govind C.] Alabama A&M Univ, Dept Biol & Environm Sci, Normal, AL 35762 USA. [Buyyarapu, Ramesh; Mc Ewan, Robert] Dow AgroSci LLC, Trait Genet & Technol, Indianapolis, IN 46268 USA. [Gu, Yong Q.] USDA ARS, Genom & Gene Discovery Res Unit, Albany, CA 94710 USA. [Lawrence, Kathy] Auburn Univ, Dept Entomol & Plant Pathol, Auburn, AL 36849 USA. RP Nyaku, ST (reprint author), Univ Ghana, Dept Crop Sci, Coll Agr & Consumer Sci, POB LG 44, Legon, Ghana. EM stnyaku@ug.edu.gh OI Buyyarapu, Ramesh/0000-0002-5343-9960 FU USDA-CSREES [2004-38814-15160]; USDA [ALAX-011-706]; NSF/PGRP [DBI 0703470]; Dow AgroSciences grant [PO 16-45462487] FX This work was supported by grants to R. V. K. and (or) G. C. S.: USDA-CSREES grant No. 2004-38814-15160, USDA grant No. ALAX-011-706, NSF/PGRP award No. DBI 0703470, and Dow AgroSciences grant PO 16-45462487. This is Journal Article No. 657 of the Alabama Agricultural Experiment Station. This study is dedicated to the late Dr. Ramesh Kantety. NR 69 TC 1 Z9 1 U1 2 U2 11 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0831-2796 EI 1480-3321 J9 GENOME JI Genome PD APR PY 2014 VL 57 IS 4 BP 209 EP 221 DI 10.1139/gen-2014-0019 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AP1JS UT WOS:000341825900004 PM 25036535 ER PT J AU Walsh, GC Mattioli, F Weber, DC AF Cabrera Walsh, G. Mattioli, Federico Weber, Donald C. TI Differential response of male and female Diabrotica speciosa (Coleoptera: Chrysomelidae) to bitter cucurbit-based toxic baits in relation to the treated area size SO INTERNATIONAL JOURNAL OF PEST MANAGEMENT LA English DT Article DE behavioural dimorphism; cucurbitacin bait; kairomones; non-target effects; phagostimulants ID CORN-ROOTWORM COLEOPTERA; CUCUMBER BEETLE COLEOPTERA; WIDE MANAGEMENT; WESTERN; INSECTICIDE; CARBARYL; STIMULANT; EXTRACTS; SLAM(R); PROGRAM AB Cucurbitacin-rich extracts are phagostimulants for both male and female Luperini, but field trials reveal that males are far more attracted to them than females. The sex ratio and number of beetles killed by an application of cucurbitacin-based toxic baits was assessed at two different scales: small areas of 100m(2), and a larger area of 10,000m(2). The small area bait applications killed mostly males (95.2%), but the 1-ha treated plot showed a lower sexual bias (57% male). An earlier study indicated point sources (sticky traps) attracted only males. Results indicate that the distance attraction to bitter cucurbit extracts is almost exclusive to males, but the wide dispersal of a toxic bait based on them should promote encounter and control of both sexes within the treated area. No significant non-target effects were observed. C1 [Cabrera Walsh, G.; Mattioli, Federico] FuEDEI Invas Species Res Fdn, Buenos Aires, DF, Argentina. [Weber, Donald C.] ARS, USDA, Invas Insect Biocontrol & Behav Lab, Beltsville, MD USA. RP Walsh, GC (reprint author), FuEDEI Invas Species Res Fdn, Bolivar 1559,B1686EFA Hurlingham, Buenos Aires, DF, Argentina. EM gcabrera@fuedei.org; federico.mattioli@syngenta.com NR 39 TC 0 Z9 0 U1 3 U2 9 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0967-0874 EI 1366-5863 J9 INT J PEST MANAGE JI Int. J. Pest Manage. PD APR-JUN PY 2014 VL 60 IS 2 BP 128 EP 135 DI 10.1080/09670874.2014.940025 PG 8 WC Entomology SC Entomology GA AO6ZV UT WOS:000341502500005 ER PT J AU Fankem, SLM Boone, SA Gaither, M Gerba, CP AF Fankem, Sonia L. M. Boone, Stephanie A. Gaither, Marlene Gerba, Charles P. TI Outbreak of Norovirus Illness in a College Summer Camp: Impact of Cleaning on Occurrence of Norovirus on Fomites SO JOURNAL OF ENVIRONMENTAL HEALTH LA English DT Article ID NORWALK VIRUS; NURSING-HOMES; GASTROENTERITIS; CONTAMINATION AB During the summer of 2005 an outbreak of norovirus acute gastroenteritis occurred in a residential college summer camp and was reported to the local health department. The outbreak spread rapidly to several other groups concurrently sharing the same facilities. During the investigation, fomites were sampled at different times in dorm rooms and tested for norovirus. The number of norovirus-positive rooms increased after the first room cleaning, from 40% to 73%. After the initial cleaning, the staff was instructed on proper cleaning and disinfection procedures and provided with disposable disinfecting wipes to reduce cross contamination, and the number of norovirus-positive rooms decreased to 30%. These findings reinforce the need for appropriate cleaning and disinfection procedures during a norovirus outbreak. C1 [Fankem, Sonia L. M.; Gerba, Charles P.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. [Boone, Stephanie A.] USDA, Washington, DC USA. [Gaither, Marlene] Coconino Hlth Dept, Dept Hlth Sci, Flagstaff, AZ USA. RP Gerba, CP (reprint author), Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. EM gerba@ag.arizona.edu NR 22 TC 5 Z9 5 U1 0 U2 5 PU NATL ENVIRON HEALTH ASSOC PI DENVER PA 720 S COLORADO BLVD SUITE 970, SOUTH TOWER, DENVER, CO 80246 USA SN 0022-0892 J9 J ENVIRON HEALTH JI J. Environ. Health PD APR PY 2014 VL 76 IS 8 BP 20 EP 26 PG 7 WC Environmental Sciences; Public, Environmental & Occupational Health SC Environmental Sciences & Ecology; Public, Environmental & Occupational Health GA AO7NS UT WOS:000341540500004 PM 24749222 ER PT J AU Brandao, CC Asquieri, ER Attaran, S Damiani, C AF Brandao, Camila Cheker Asquieri, Eduardo Ramirez Attaran, Shireen Damiani, Clarissa TI Study of the aging of fermented of yacon (Smallanthus sonchifolius) and sensory profile and acceptance SO FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE alcoholic beverage; fermented fruit; fructooligosaccharide; antioxidant capacity ID ACETIC-ACID BACTERIA; VITIS-VINIFERA L.; RED WINES; ANTIOXIDANT ACTIVITY; PHENOLIC COMPOSITION; INSTRUMENTAL ANALYSIS; VOLATILE COMPOUNDS; STORAGE POSITION; BOTTLE STORAGE; FRUIT WINE AB Yacon is considered a functional food due to its the fructooligosaccharide (FOS) content, however its perishability and low production volume is a problem. The aim of this study was to analyze the changes in aging during one year of storage and conduct sensory analysis of fermented of yacon. For one year total acidity, volatile acidity, free and total sulfur dioxide, reducing sugars, sucrose, phenols and FOS and its antioxidant power were studied. At the end of aging a sensory profile and acceptance panel was performed. The total and volatile acidity increased significantly (p < 0.05). A decrease in fructooligosaccharide was also observed, indicating that yeasts are probably capable of hydrolyzing the latter. The total sulfur dioxide decreased significantly, demonstrating its ability to act well against oxidation products. This product showed good antioxidant capacity and sensory profiles of considerable acceptance. Therefore it can be affirmed that the alcoholic fermentation of yacon can be a good alternative for the industrial sector and farmers in the region could be encouraged to use large-scale production. C1 [Brandao, Camila Cheker; Asquieri, Eduardo Ramirez; Damiani, Clarissa] Univ Fed Goias, Fac Agron, Goiania, Go, Brazil. [Attaran, Shireen] USDA, Berkeley, CA USA. RP Brandao, CC (reprint author), Univ Fed Goias, Fac Agron, Goiania, Go, Brazil. EM camilacheker@yahoo.com.br NR 54 TC 0 Z9 0 U1 2 U2 18 PU SOC BRASILEIRA CIENCIA TECNOLOGIA ALIMENTOS PI CAMPINAS PA AV BRASIL 2880, CAXIA POSTAL 271 CEP 13001-970, CAMPINAS, SAO PAULO 00000, BRAZIL SN 0101-2061 EI 1678-457X J9 FOOD SCI TECH-BRAZIL JI Food Sci. Technol. PD APR-JUN PY 2014 VL 34 IS 2 BP 324 EP 331 DI 10.1590/S0101-20612014005000032 PG 8 WC Food Science & Technology SC Food Science & Technology GA AN4ZP UT WOS:000340598900016 ER PT J AU Nicholson, EM Greenlee, JJ Vrentas, CE AF Nicholson, Eric M. Greenlee, Justin J. Vrentas, Catherine E. TI Effects of a naturally occurring amino acid substitution in bovine PrP: A novel model for inherited prion disease in a natural host species SO PRION LA English DT Meeting Abstract C1 [Nicholson, Eric M.; Greenlee, Justin J.; Vrentas, Catherine E.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1933-6896 EI 1933-690X J9 PRION JI Prion PD APR-JUN PY 2014 VL 8 SU S MA P.175 BP 107 EP 107 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AN5FD UT WOS:000340614900230 ER PT J AU Koga, LJ Bowen, CR Godoy, CV de Oliveira, MCN Hartman, GL AF Koga, Lucimara Junko Bowen, Charles Roger Godoy, Claudia Vieira Neves de Oliveira, Maria Cristina Hartman, Glen Lee TI Mycelial compatibility and aggressiveness of Sclerotinia sclerotiorum isolates from Brazil and the United States SO PESQUISA AGROPECUARIA BRASILEIRA LA English DT Article DE Glycine max; Sclerotinia stem rot; disease resistance; screening of cultivars; white mold ID SOYBEAN CULTIVARS; STEM ROT; PARTIAL RESISTANCE; POPULATIONS; FIELD; WASHINGTON; SUNFLOWER; PATHOGEN; LETTUCE AB The objective of this work was to evaluate the genetic diversity among Sclerotinia sclerotiorum isolates from Brazil and the USA, assess their aggressiveness variability, and verify the existence of an isolate-cultivar interaction. Isolate variability was determined by mycelial compatibility grouping (MCG), and isolate aggressiveness by cut-stem inoculations of soybean cultivars. Two experiments for MCGs and two for aggressiveness were conducted with two sets of isolates. The first set included nine isolates from the same soybean field in Brazil and nine from the Midwest region of the USA. The second set included 16 isolates from several regions of Brazil and one from the USA. In the first set, 18 isolates formed 12 different MCGs. In the second set, 81% of the isolates from Brazil grouped into a single MCG. No common MCGs were observed among isolates from Brazil and the USA. The isolates showed aggressiveness differences in the first set, but not in the second. Although aggressiveness differed in the first set, soybean cultivars and isolates did not interact significantly. Cultivar rank remained the same, regardless of the genetic diversity, aggressiveness difference, and region or country of origin of the isolate. Results from screening of soybean cultivars, performed by the cut-stem method in the USA, can be used as reference for researchers in Brazil. C1 [Koga, Lucimara Junko; Hartman, Glen Lee] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Bowen, Charles Roger] ARS, USDA, Urbana, IL 61801 USA. [Godoy, Claudia Vieira; Neves de Oliveira, Maria Cristina] Embrapa Soja, BR-86001970 Londrina, PR, Brazil. RP Koga, LJ (reprint author), Univ Illinois, Dept Crop Sci, 1101 West Peabody Dr, Urbana, IL 61801 USA. EM ljk3001@yahoo.com.br; crbowen@illinois.edu; claudia.godoy@embrapa.br; mcristina.oliveira@embrapa.br; ghartman@illinois.edu FU Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) FX To Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), for fellowship granted; to Dr. Mauricio Meyer, for providing S. sclerotiorum isolates from Brazilian Cerrado region. NR 34 TC 1 Z9 1 U1 1 U2 18 PU EMPRESA BRASIL PESQ AGROPEC PI BRASILIA DF PA EMBRAPA INFORMACAO TECNOLOGICA, PESQUISA AGROPECUARIA BRASILEIRA - PAB, CAIXA POSTAL 040315, 70770-901 BRASILIA DF, BRAZIL SN 0100-204X EI 1678-3921 J9 PESQUI AGROPECU BRAS JI Pesqui. Agropecu. Bras. PD APR PY 2014 VL 49 IS 4 BP 265 EP 272 DI 10.1590/S0100-204X2014000400004 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA AN3MB UT WOS:000340491100004 ER PT J AU Chantre, GR Blanco, AM Forcella, F Van Acker, RC Sabbatini, MR Gonzalez-Andujar, JL AF Chantre, G. R. Blanco, A. M. Forcella, F. Van Acker, R. C. Sabbatini, M. R. Gonzalez-Andujar, J. L. TI A comparative study between non-linear regression and artificial neural network approaches for modelling wild oat (Avena fatua) field emergence SO JOURNAL OF AGRICULTURAL SCIENCE LA English DT Article ID SEEDLING EMERGENCE; WEED EMERGENCE; HYDROTHERMAL TIME; DORMANCY; GERMINATION; DYNAMICS; MANAGEMENT; TEMPERATURE; TILLAGE; SYSTEMS AB Non-linear regression (NLR) techniques are used widely to fit weed field emergence patterns to soil microclimatic indices using S-type functions. Artificial neural networks (ANNs) present interesting and alternative features for such modelling purposes. In the present work, a univariate hydrothermal-time based Weibull model and a bivariate (hydro-time and thermal-time) ANN were developed to study wild oat emergence under non-moisture restriction conditions using data from different locations worldwide. Results indicated a higher accuracy of the neural network in comparison with the NLR approach due to the improved descriptive capacity of thermal-time and the hydro-time as independent explanatory variables. The bivariate ANN model outperformed the conventional Weibull approach, in terms of RMSE of the test set, by 70.8%. These outcomes suggest the potential applicability of the proposed modelling approach in the design of weed management decision support systems. C1 [Chantre, G. R.; Sabbatini, M. R.] Univ Nacl Sur, CONICET, Dept Agron CERZOS, RA-8000 Bahia Blanca, Buenos Aires, Argentina. [Blanco, A. M.] Univ Nacl Sur, CONICET, RA-8000 Bahia Blanca, Buenos Aires, Argentina. [Forcella, F.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Van Acker, R. C.] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Gonzalez-Andujar, J. L.] CSIC, Inst Agr Sostenible, Cordoba 14080, Spain. RP Gonzalez-Andujar, JL (reprint author), CSIC, Inst Agr Sostenible, Aptdo 4084, Cordoba 14080, Spain. EM andujar@cica.es RI GONZALEZ-ANDUJAR, JOSE /G-5671-2013 OI GONZALEZ-ANDUJAR, JOSE /0000-0003-2356-4098 FU Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET PIP) [11220100100222]; Universidad Nacional del Sur [PGI 24/A157]; FEDER funds; Spanish Ministry of Economy and Competitiveness [AGL2009-7883] FX This research was partially supported by grants from Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET PIP No 11220100100222) and Universidad Nacional del Sur (PGI 24/A157) to G.R.C., A.M.B. and M.R.S. and by grants from FEDER funds and the Spanish Ministry of Economy and Competitiveness (AGL2009-7883) to J.L.G.-A. NR 43 TC 4 Z9 4 U1 0 U2 8 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0021-8596 EI 1469-5146 J9 J AGR SCI JI J. Agric. Sci. PD APR PY 2014 VL 152 IS 2 BP 254 EP 262 DI 10.1017/S0021859612001098 PG 9 WC Agriculture, Multidisciplinary SC Agriculture GA AM0DH UT WOS:000339513000007 ER PT J AU Dooley, JW Smith-Pardo, A AF Dooley, John W., III Smith-Pardo, Allan TI New distribution and host records of four whiteflies (Hemiptera: Aleyrodidae: Aleyrodinae) intercepted on Magnolia (Magnoliaceae) at US ports of entry SO PAN-PACIFIC ENTOMOLOGIST LA English DT Article DE Aleuroclava; Cockerelliella; Quarantine; Aleyrodinae ID COCKERELLIELLA SUNDARARAJ; INDIA AB New distribution and host records are provided for four whiteflies intercepted at U.S. ports of entry on propagative material of Magnolia from Asia. Aleuroclava latus (Takahashi, 1934), Aleuroclava nitidus (Singh, 1932), and Cockerelliella meghalayensis Sundararaj & David, 1992 were intercepted on the leaves of Magnolia spp. (Magnoliaceae) from Thailand. Cockerelliella indica Sundararaj & David, 1932 was intercepted on the leaves of Magnolia yunnanensis (Hu) Nooteboom from China. An identification key to the puparia of the whitefiies found on Magnolia species is included along with revised descriptions and illustrations of all the species based on the specimens intercepted at U.S. ports of entry. C1 [Dooley, John W., III; Smith-Pardo, Allan] Anim & Plant Hlth Inspect Serv, USDA, San Francisco, CA 94080 USA. RP Smith-Pardo, A (reprint author), Anim & Plant Hlth Inspect Serv, USDA, 389 Oyster Point Blvd,Suite 2A, San Francisco, CA 94080 USA. EM allan.h.smith-pardo@aphis.usda.gov NR 33 TC 0 Z9 0 U1 0 U2 0 PU PACIFIC COAST ENTOMOL SOC PI SAN FRANCISCO PA C/O CALIFORNIA ACADEMY SCIENCES, 875 HOWARD STREET, SAN FRANCISCO, CA 94103-3009 USA SN 0031-0603 EI 2162-0237 J9 PAN-PAC ENTOMOL JI Pan-Pacific Entomol. PD APR PY 2014 VL 90 IS 2 BP 82 EP 99 PG 18 WC Entomology SC Entomology GA AL9JT UT WOS:000339458100005 ER PT J AU Shwab, EK Zhu, XQ Majumdar, D Pena, HFJ Gennari, SM Dubey, JP Su, CL AF Shwab, E. Keats Zhu, Xing-Quan Majumdar, Debashree Pena, Hilda F. J. Gennari, Solange M. Dubey, Jitender P. Su, Chunlei TI Geographical patterns of Toxoplasma gondii genetic diversity revealed by multilocus PCR-RFLP genotyping SO PARASITOLOGY LA English DT Review DE Toxoplasma gondii; PCR-RFLP; genotyping; population structure; genetic diversity ID POPULATION-STRUCTURE; MOUSE-VIRULENCE; SEA OTTERS; ANTIBODIES; INFECTION; STRAINS; BRAZIL; IDENTIFICATION; SCHIZOPHRENIA; TRANSMISSION AB In recent years, an extensive collection of Toxoplasma gondii samples have been typed using a set of 10 PCR-RFLP genetic markers. Here we summarize the data reported until the end of 2012. A total of 1457 samples were typed into 189 genotypes. Overall, only a few genotypes dominate in the northern hemisphere, which is in stark contrast to the southern hemisphere where hundreds of genotypes coexist with none being notably dominant. PCR-RFLP genotype #1 (Type II clonal), #2 (Type III), #3 (Type II variant) and #10 (Type I) are identified globally. Genotypes #2 and #3 dominate in Africa, genotypes #9 (Chinese 1) and #10 are prevalent in Asia, genotypes #1, #2 and #3 are prevalent in Europe, genotypes #1, #2, #3, #4 and #5 dominate in North America (#4 and #5 are collectively known as Type 12). In Central and South America, there is no clear dominance of any genotype even though a few have relatively higher frequencies. Statistical analysis indicates significant differences among populations in Africa, Asia, Europe, North America, and Central and South America, with only Europe and North America exhibiting similar diversity. Collectively, the results revealed distinct population structures and geographical patterns of diversity in T. gondii. C1 [Shwab, E. Keats; Majumdar, Debashree; Su, Chunlei] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. [Zhu, Xing-Quan; Su, Chunlei] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China. [Pena, Hilda F. J.; Gennari, Solange M.] Univ Sao Paulo, Fac Vet Med, Sao Paulo, Brazil. [Dubey, Jitender P.] USDA, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Su, CL (reprint author), Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. EM csu1@utk.edu RI Su, Chunlei/M-1892-2013 OI Su, Chunlei/0000-0001-8392-7108 FU National Natural Science Foundation of China [31228022, 31230073]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil FX This work was supported in part by the National Natural Science Foundation of China (Grant No. 31228022 to CS, XQZ and 31230073 to XQZ), and scholarships from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil (HFJP, SMG). NR 42 TC 61 Z9 66 U1 2 U2 21 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0031-1820 EI 1469-8161 J9 PARASITOLOGY JI Parasitology PD APR PY 2014 VL 141 IS 4 BP 453 EP 461 DI 10.1017/S0031182013001844 PG 9 WC Parasitology SC Parasitology GA AM1OY UT WOS:000339618200001 PM 24477076 ER PT J AU O'Dea, CB AF O'Dea, Claire Berger TI THE RELATIONSHIP BETWEEN COASTAL PLAIN POND VEGETATION AND ENVIRONMENT AT LOCAL AND BROAD SPATIAL SCALES SO RHODORA LA English DT Article DE freshwater wetland; pond shore; coastal plain pond; environmental gradients; community composition ID ZONATION PATTERNS; PLANT ZONATION; NOVA-SCOTIA; COMPETITIVE ABILITY; GRADIENT ANALYSIS; VERNAL POOLS; WATER-LEVEL; NEW-ENGLAND; SALT-MARSH; WETLANDS AB Coastal plain ponds support many plant species that have been designated as threatened due to human alteration of the hydrology and nutrient availability. Understanding how these species respond to environmental gradients at multiple spatial scales can support conservation efforts. I examined the relationship between plant species composition and environmental variables that operate at two spatial scales: within (local-scale) and among (broad-scale) 18 coastal plain ponds on the island of Martha's Vineyard, Massachusetts. Patterns in species composition occurred at both local and broad scales. Elevation along the water-depth gradient was the only local factor strongly correlated with species composition. Significant broad-scale environmental factors included surficial geology, hydrology, and specific conductance of pond water. Overall patterns in vegetation species composition and abundance were more closely related to broad-scale environmental variables than to local environmental variables, suggesting that pondshore vegetation is largely determined by variables that are expressed at the broad scale. Conservation organizations concerned with protecting the coastal plain pond ecosystem should, therefore, protect a network of ponds that captures the full range of environmental variability, because ponds that vary in hydrologic regime, surficial geology, and water salinity exhibit significantly different patterns in species composition. C1 USDA, Forest Serv, Arlington, VA 22203 USA. RP O'Dea, CB (reprint author), USDA, Forest Serv, 3835 9th St N 304W, Arlington, VA 22203 USA. EM cbodea@fs.fed.us FU Society of Wetland Scientists; Sigma Xi; Woods Hole Sea Grant FX I sincerely thank Drs. Norm Christensen, Chris Neill, Dan Richter, and Dean Urban for comments on the manuscript. I would also like to thank Tom Chase and Matt Pelikan at The Nature Conservancy, Dick Johnson at Sheriff's Meadow Foundation, Julie Russell at Martha's Vineyard Land Bank, Bill Wilcox at the Martha's Vineyard Commission, Glenn Provo at Vineyard Land Surveying, and private landowners on Martha's Vineyard for providing me with guidance and logistical support. This research was further supported by funding from Society of Wetland Scientists, Sigma Xi, and Woods Hole Sea Grant. NR 65 TC 0 Z9 0 U1 6 U2 13 PU NEW ENGLAND BOTANICAL CLUB INC PI LAWRENCE PA P.O. BOX 368, LAWRENCE, KS 66044 USA SN 0035-4902 EI 1938-3401 J9 RHODORA JI Rhodora PD APR PY 2014 VL 116 IS 966 BP 187 EP 223 DI 10.3119/12-17 PG 37 WC Plant Sciences SC Plant Sciences GA AL1FS UT WOS:000338871800004 ER PT J AU Zhao, M Ramage, J Semmens, K Obleitner, F AF Zhao, Meng Ramage, Joan Semmens, Kathryn Obleitner, Friedrich TI Recent ice cap snowmelt in Russian High Arctic and anti-correlation with late summer sea ice extent SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE Novaya Zemlya; Severnaya Zemlya; glacier; snowmelt; passive microwave; active microwave; remote sensing ID SOUTHEAST-ALASKAN ICEFIELDS; MASS-BALANCE; SEVERNAYA-ZEMLYA; MELT DETECTION; CLIMATE-CHANGE; LEVEL RISE; GLACIERS; RECORD; GREENLAND; COVER AB Glacier surface melt dynamics throughout Novaya Zemlya (NovZ) and Severnaya Zemlya (SevZ) serve as a good indicator of ice mass ablation and regional climate change in the Russian High Arctic. Here we report trends of surface melt onset date (MOD) and total melt days (TMD) by combining multiple resolution-enhanced active and passive microwave satellite datasets and analyze the TMD correlations with local temperature and regional sea ice extent. The glacier surface snowpack on SevZ melted significantly earlier (-7.3 days/decade) from 1992 to 2012 and significantly longer (7.7 days/decade) from 1995 to 2011. NovZ experienced large interannual variability in MOD, but its annual mean TMD increased. The snowpack melt on NovZ is more sensitive to temperature fluctuations than SevZ in recent decades. After ruling out the regional temperature influence using partial correlation analysis, the TMD on both archipelagoes is statistically anti-correlated with regional late summer sea ice extent, linking land ice snowmelt dynamics to regional sea ice extent variations. C1 [Zhao, Meng; Ramage, Joan; Semmens, Kathryn] Lehigh Univ, Dept Earth & Environm Sci, Bethlehem, PA 18015 USA. [Zhao, Meng] Univ Calif Irvine, Dept Earth Syst Sci, Irvine, CA 92617 USA. [Semmens, Kathryn] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Obleitner, Friedrich] Univ Innsbruck, Inst Meteorol & Geophys, A-6020 Innsbruck, Austria. RP Zhao, M (reprint author), Lehigh Univ, Dept Earth & Environm Sci, 1 W Packer Ave, Bethlehem, PA 18015 USA. EM ramage@lehigh.edu RI Obleitner, Friedrich/H-2657-2012 OI Obleitner, Friedrich/0000-0001-8520-8684 FU Lehigh University; NASA by the European Commission [NNX11AR14G, ENV4-CT97-0490]; Austrian Science Fund [I 369-B17] FX We thank the Microwave Earth Remote Sensing Laboratory at Brigham Young University (MERSL-BYU) for use of resolution enhanced passive and active microwave data. Special thanks to Thomas Opel, Benjamin Felzer, Dork Sahagian, Matthew Pritchard and Christopher Shuman for helpful discussions, Diedrich Fritsche for assistance with field work and two anonymous reviewers for suggestions that improved the manuscript. This study was supported by Lehigh University, by NASA Grant #NNX11AR14G by the European Commission, contract ENV4-CT97-0490, and the Austrian Science Fund grant I 369-B17, respectively. USDA is an equal opportunity provider and employer. NR 69 TC 7 Z9 7 U1 3 U2 17 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD APR PY 2014 VL 9 IS 4 AR 045009 DI 10.1088/1748-9326/9/4/045009 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AK9XD UT WOS:000338781000023 ER PT J AU Thomson, SJ Huang, Y AF Thomson, Steven J. Huang, Yanbo TI Portable device to assess dynamic accuracy of global positioning system (GPS) receivers used in agricultural aircraft SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE GPS receiver accuracy; agricultural aircraft; aerial application; remote sensing; georeferencing; UAV ID AERIAL APPLICATION SYSTEM AB A device was designed to test the dynamic accuracy of Global Positioning System (GPS) receivers used in agricultural aircraft and other aerial vehicles. The system works by directing a sun-reflected light beam from the ground to the aircraft using mirrors. A photo detector points downward from the aircraft to detect the light beam, and photo detection circuitry triggers an event in the guidance system data file at the aircraft's location corresponding to the precisely georeferenced position on the ground. Construction details are presented on the mirror-based light reflection system and photo-electronic circuitry designed to trigger an event in the guidance system's log file. An example application evaluated the horizontal accuracy of a stand-alone GPS receiver by matching dynamic data with data from the aircraft's guidance system. Results indicated a 2.16 s lead in position registered by the stand-alone receiver over that registered by the aircraft's guidance system GPS receiver, which had been previously evaluated to be within 0.13 s of Real-Time Kinematic (RTK)-referenced time and position. C1 [Thomson, Steven J.; Huang, Yanbo] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Thomson, SJ (reprint author), USDA ARS, Crop Prod Res Unit, 141 Expt Stn Rd, Stoneville, MS 38776 USA. EM steve.thomson@ars.usda.gov; yanbo.huang@ars.usda.gov NR 10 TC 1 Z9 1 U1 1 U2 8 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD APR PY 2014 VL 7 IS 2 BP 68 EP 74 DI 10.3965/j.ijabe.20140702.008 PG 7 WC Agricultural Engineering SC Agriculture GA AK2RP UT WOS:000338267500008 ER PT J AU Pei, W Lan, YB Luo, XW Zhou, ZY Wang, ZG Wang, YH AF Pei, Wang Lan, Yubin Luo Xiwen Zhou Zhiyan Wang, Zhigang Wang, Yonghui TI Integrated sensor system for monitoring rice growth conditions based on unmanned ground vehicle system SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE unmanned ground vehicle system (UGVS); multi-sensors; rice growth condition; spectral vegetation indices; leaf area index (LAI) ID SPECTRAL VEGETATION INDEXES; NITROGEN STATUS; PRECISION AGRICULTURE; CHLOROPHYLL STATUS; CROP MANAGEMENT; FIELD-LEVEL; REFLECTANCE; WHEAT; CORN AB Ground-based platform systems provide a good tool for monitoring and managing crop conditions in precision agriculture applications and have been widely used for monitoring crop conditions. To develop an unmanned ground vehicle system (UGVS) based multi-sensors and test the feasibility of this system for monitoring rice conditions, an UGVS was developed to collect real-time rice condition information including NDVI values, reflectance measurements and crop canopy temperature in this study. Major components of the integrated system are GreenSeeker R100 system, hyper-spectroradiometer and infrared temperature sensor. The leaf area index (LAI) was measured by the CGMD302 Spectrometer. The Independent Samples T-Test method and the one way ANOVA method were used to determine the best spectral indices and analyze the relationship between the vegetation indices and rice LAI. It was found that the two best spectral indices for estimating LAI were NDVI (860 nm and 750 nm) with the correlation coefficient (R-2) at 0.745 and RVI (853 nm and 751 nm) with the R2 at 0.724. The results show the UGVS can support multi-source information acquisition and is useful for crop management and precision agriculture applications. C1 [Pei, Wang; Luo Xiwen; Zhou Zhiyan] South China Agr Univ, Key Lab Key Technol Agr Machine & Equipment, Minist Educ, Guangzhou 510642, Guangdong, Peoples R China. [Lan, Yubin; Wang, Zhigang] USDA ARS, AATRU, College Stn, TX 77845 USA. [Wang, Yonghui] Prairie View A&M Univ, Prairie View, TX 77446 USA. RP Luo, XW (reprint author), South China Agr Univ, Key Lab Key Technol Agr Machine & Equipment, Minist Educ, Guangzhou 510642, Guangdong, Peoples R China. EM wangpei830624@163.com; yubin.lan@ars.usda.gov; xwluo@scau.edu.cn; zyzhou@scau.edu.cn; 95858325@qq.com; yowang@pvamu.edu NR 37 TC 2 Z9 2 U1 1 U2 37 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD APR PY 2014 VL 7 IS 2 BP 75 EP 81 DI 10.3965/j.ijabe.20140702.009 PG 7 WC Agricultural Engineering SC Agriculture GA AK2RP UT WOS:000338267500009 ER PT J AU Nafus, AM Davies, KW AF Nafus, Aleta M. Davies, Kirk W. TI Medusahead Ecology and Management: California Annual Grasslands to the Intermountain West SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Review DE Control; exotic annual grass; invasive plant prevention; restoration; revegetation ID TAENIATHERUM-CAPUT-MEDUSAE; EXOTIC ANNUAL GRASS; NATIVE PERENNIAL GRASSES; INVASIVE ANNUAL GRASS; SAGEBRUSH STEPPE; GREAT-BASIN; BIOLOGICAL INVASIONS; PLANT-COMMUNITIES; BLUEBUNCH WHEATGRASS; ELYMUS-ELYMOIDES AB The spread of medusahead across the western United States has severe implications for a wide range of ecosystem services. Medusahead invasion reduces biodiversity, wildlife habitat and forage production, and often leads to increased fire frequency and restoration costs. Medusahead is problematic in the Intermountain West and California Annual Grasslands. The last review of medusahead ecology and management was completed 20 years ago. Since the last review, there have been scientific advances in medusahead management suggesting a significant need to develop an up-to-date synthesis. Medusahead continues to pose a serious threat to rangeland ecosystems. In this synthesis, we present new information regarding the ecology of medusahead, suggest a framework for managing medusahead based on invasion level, and identify research needs to further improve management of this invasive annual grass. Success of different management practices varies between the Intermountain West and California Annual Grasslands, signifying that the best management practices are those specifically tailored with consideration of climate, soil, plant community characteristics, and management objectives. Prevention and control treatments that are useful in the Intermountain West may not be practical or effective in the California Annual Grasslands and vice-versa. C1 [Nafus, Aleta M.] Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. [Davies, Kirk W.] ARS, USDA, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Nafus, AM (reprint author), Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. EM Aleta.Nafus@oregonstate.edu FU NRCS FX The authors would like to thank the NRCS for providing some of the financial support for this project. Drs. B. Smith, M. McClaran and G. Kyser provided many helpful comments to improve this manuscript. NR 114 TC 8 Z9 8 U1 4 U2 72 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2014 VL 7 IS 2 BP 210 EP 221 DI 10.1614/IPSM-D-13-00077.1 PG 12 WC Plant Sciences SC Plant Sciences GA AK5PT UT WOS:000338479000002 ER PT J AU Davies, KW Johnson, DD Nafus, AM AF Davies, Kirk W. Johnson, Dustin D. Nafus, Aleta M. TI Restoration of Exotic Annual Grass-Invaded Rangelands: Importance of Seed Mix Composition SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Bunchgrass; crested wheatgrass; diversity; dominant species; medusahead; seeding; species richness ID TAENIATHERUM-CAPUT-MEDUSAE; INVASIVE ALIEN PLANTS; GREAT-BASIN; SAGEBRUSH STEPPE; BROMUS-TECTORUM; SAGE-GROUSE; VEGETATION CHARACTERISTICS; CRESTED WHEATGRASS; BIOMASS PRODUCTION; DIVERSITY AB Restoration of exotic annual grass-invaded rangelands is needed to improve ecosystem function and services. Increasing plant species richness is generally believed to increase resistance to invasion and increase desired vegetation. However, the effects of species richness and individual plant life forms in seed mixes used to restore rangelands invaded by exotic annual grasses have not been investigated. We evaluated the effects of seeding different life forms and increasing species richness in seed mixes seeded after exotic annual grass control to restore desirable vegetation (perennial herbaceous vegetation) and limit exotic annual grasses at two sites in southeastern Oregon. We also investigated the effects of seeding two commonly used perennial grasses individually and together on plant community characteristics. Large perennial grasses, the dominant herbaceous plant life form, were the most important group to seed for increasing perennial herbaceous vegetation cover and density. We did not find evidence that greater seed mix species richness increased perennial herbaceous vegetation or decreased exotic annual grass dominance more than seeding only the dominant species. None of the seed mixes had a significant effect on exotic annual grass cover or density, but the lack of a measured effect may have been caused by low annual grass propagule pressure in the first couple of years after annual grass control and an unusually wet-cool spring in the third year post-seeding. Although our results suggest that seeding only the dominant plant life form will likely maximize plant community productivity and resistance to invasion in exotic annual grass-invaded northern Great Basin arid rangelands, seeding a species rich seed mix may have benefits to higher tropic levels and community stability. Clearly the dominant species are the most prudent to include in seed mixes to restore exotic annual grass-invaded plant communities, especially with finite resources and an increasingly large area in need of restoration. C1 [Davies, Kirk W.] ARS, USDA, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. [Johnson, Dustin D.; Nafus, Aleta M.] Oregon State Univ, Dept Anim & Rangeland Sci, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Davies, KW (reprint author), ARS, USDA, Eastern Oregon Agr Res Ctr, 67826-A Hwy 205, Burns, OR 97720 USA. EM kirk.davies@oregonstate.edu NR 58 TC 3 Z9 3 U1 4 U2 52 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2014 VL 7 IS 2 BP 247 EP 256 DI 10.1614/IPSM-D-12-00093.1 PG 10 WC Plant Sciences SC Plant Sciences GA AK5PT UT WOS:000338479000006 ER PT J AU Aracena, P Chung, W Jones, G AF Aracena, Pablo Chung, Woodam Jones, Greg TI Landscape-Level Simulation of Weed Treatments to Evaluate Treatment Plan Options SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Noxious weeds; weed control; weed spread modeling ID STRATEGIES AB Models have been developed to simulate the long-term effects of weed treatments across a landscape to determine effective management strategies, but those models might not be suitable for evaluating short-term action plans of weed treatments that are specific in time and place. In this study, we developed a simulation model to build and evaluate 5-yr weed treatment plan options in terms of their cost and effectiveness in minimizing total infestation areas over the short-term planning horizon. In an iterative, interactive process, 5-yr treatment plan options are developed based on user-defined weed treatment preferences, and evaluated in terms of total projected infestation areas at the end of the planning horizon. The simulation model was applied to a study area of 24,867 ha (61,447 ac) located in the Salmon River watershed in Idaho. Eight treatment plan options were developed using two treatment priority strategies and four increasing budget levels, and compared for their effectiveness. The application results showed that regardless of budget levels, site priority strategies were more cost-effective than the species priority strategies in reducing total infestation areas over time. This simulation model can provide weed managers with a useful tool to evaluate short-term treatment options, and thus support informed decision-making for effective weed management. Although the availability and quality of input data may be a practical limitation of using the simulation model, more data would become available and improved as more invasive species monitoring programs are implemented. C1 [Aracena, Pablo; Chung, Woodam] Univ Montana, Dept Forest Management, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Jones, Greg] ARS, Human Dimens Sci Program, Rocky Mt Res Stn, USDA, Missoula, MT 59807 USA. RP Chung, W (reprint author), Univ Montana, Dept Forest Management, Coll Forestry & Conservat, 32 Campus Dr, Missoula, MT 59812 USA. EM woodam.chung@umontana.edu FU U.S. Department of Agriculture under USDA/ERS/PREISM [58-7000-6-0082]; Utpal Vasavada of the USDA Economic Research Service; Pat Green of the Nez Perce National Forest; Carl Crabtree of the Salmon River Weed Management Area; Gil Gale of the Bitterroot National Forest FX Funding for this research was provided by the U.S. Department of Agriculture under USDA/ERS/PREISM Cooperative Agreement No. 58-7000-6-0082. The authors thank Utpal Vasavada of the USDA Economic Research Service, Pat Green of the Nez Perce National Forest, Carl Crabtree of the Salmon River Weed Management Area, and Gil Gale of the Bitterroot National Forest for their support, comments and data provided for this research. We also thank Dr. Tim Prather of University of Idaho and Peter Rice of University of Montana for their collaboration and data provision for the applications developed in this study. Finally, we greatly appreciate the associate editor and two anonymous reviewers for helpful comments and suggestions that improved the paper significantly. NR 7 TC 1 Z9 1 U1 1 U2 4 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2014 VL 7 IS 2 BP 278 EP 290 DI 10.1614/IPSM-D-13-00043.1 PG 13 WC Plant Sciences SC Plant Sciences GA AK5PT UT WOS:000338479000009 ER PT J AU Uselman, SM Snyder, KA Leger, EA Duke, SE AF Uselman, Shauna M. Snyder, Keirith A. Leger, Elizabeth A. Duke, Sara E. TI First-Year Establishment, Biomass and Seed Production of Early vs. Late Seral Natives in Two Medusahead (Taeniatherum caput-medusae) Invaded Soils SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Exotic annual grass; first-year establishment; functional traits; native plants; restoration; plant-soil relationships ID CHEATGRASS BROMUS-TECTORUM; GREAT-BASIN; GERMINATION RESPONSE; SAGEBRUSH STEPPE; RESOURCE AVAILABILITY; GRASSLAND RESTORATION; PLANT-COMMUNITIES; ELYMUS-ELYMOIDES; PERENNIAL GRASS; INVASION AB Re-seeding efforts to restore or rehabilitate Great Basin rangelands invaded by exotic annual grasses are expensive and have generally achieved limited success. There is a need to identify new strategies to improve restoration outcomes. We tested the performance of a native early seral seed mix (annual forbs, early seral grasses and shrubs) with that of a native late seral mix representative of species commonly used in restoration when growing with medusahead in soils of contrasting texture (sandy loam and clay loam) through the first growing season after seeding. Natives were also seeded without medusahead. We found that the grasses and forbs in the early seral mix established significantly better than those in the late seral mix, and the early seral mix significantly reduced aboveground biomass and seed production of medusahead by 16 and 17% respectively, likely because of competition with the early seral native forb, bristly fiddleneck. Medusahead performance was reduced in both soil types, suggesting utility of bristly fiddleneck in restoration is not limited to only one soil type. In contrast, the late seral mix did not suppress medusahead establishment, aboveground biomass or seed production. Although the native perennial grasses, particularly early seral species, were able to establish with medusahead, these grasses did not appear to have a suppressive effect on medusahead during the first growing season. Medusahead was able to establish and produce seeds on both soil types, demonstrating an ability to expand its current range in the Intermountain West, though aboveground biomass and seed production was higher in the clay loam. Our results suggest that certain species may play a key role in restoration, and that targeting early seral species in particular to find additional native species with the ability to suppress exotic annual grasses is an important next step in improving restoration outcomes in desert ecosystems. C1 [Uselman, Shauna M.; Snyder, Keirith A.] ARS, USDA, Great Basin Rangeland Res Unit, Reno, NV 89512 USA. [Leger, Elizabeth A.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. [Duke, Sara E.] ARS, USDA, Southern Plains Area Off, College Stn, TX 77845 USA. RP Uselman, SM (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89557 USA. EM s.uselman@sbcglobal.net FU Great Basin Native Plant Selection and Increase Project, U.S. Forest Service FX This research was partially supported by the Great Basin Native Plant Selection and Increase Project, U.S. Forest Service. We thank Nancy Shaw, who made the completion of this project possible. This research was conducted at the Nevada Agricultural Experiment Station, University of Nevada-Reno (UNR), and we thank Bo Kindred and his staff. Dr. T. Forbis contributed to the initial design of this study. We also thank Dr. E. Goergen for many helpful conversations. We appreciate the assistance of S. Lencioni and S. Li in data collection; C. Bacher, M. Newell, A. Smith, M. Stout, B. Wehan, and J. Whipple also helped collect data. S. Swim, B. Raitter, T. Jones, D. Harmon, and T. Bobo assisted with seed and/or soil collection. T. Jones, C. Clements, S. Li, B. Raitter, and D. Harmon helped in implementing the common garden. We thank A.J. Tiehm of the UNR Herbarium for verifying plant identification. We also thank the editor and anonymous reviewers for comments on an earlier version of this manuscript. NR 58 TC 4 Z9 4 U1 1 U2 24 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2014 VL 7 IS 2 BP 291 EP 302 DI 10.1614/IPSM-D-13-00068.1 PG 12 WC Plant Sciences SC Plant Sciences GA AK5PT UT WOS:000338479000010 ER PT J AU Gibson, DM Vaughan, RH Biazzo, J Milbrath, LR AF Gibson, Donna M. Vaughan, Richard H. Biazzo, Jeromy Milbrath, Lindsey R. TI Exploring the Feasibility of Sclerotium rolfsii VrNY as a Potential Bioherbicide for Control of Swallowworts (Vincetoxicum spp.) SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Biological control; dog-strangling vine; host specificity; invasive weeds; mycoherbicide ID BIOLOGICAL-CONTROL AGENT; OXALIC-ACID PRODUCTION; NORTH-AMERICA; CYNANCHUM-ROSSICUM; MYCELIAL BIOMASS; NEW-YORK; WORT; NIGRUM; WEEDS; MYCOHERBICIDE AB Pale swallowwort (PSW) and black swallowwort (BSW) are two viney milkweeds native to Europe that have increasingly become problematic and noxious weeds in eastern North America. An indigenous fungal isolate, Sclerotium rolfsii VrNY, was discovered causing significant mortality in a dense stand of PSW in a park in upstate New York. Although this fungus is a known pathogen with a broad host range, we evaluated the host potential of S. rolfsii VrNY on a limited range of related and nonrelated U.S. species as a critical first step to assess its suitability as a mycoherbicide for PSW and BSW. In addition, PSW and BSW produce the specific stereoisomer (-)-antofine, a compound with antimicrobial and phytotoxic activity that could inhibit the pathogen. Tests revealed this compound had no effect on S. rolfsii VrNY. This isolate caused significant mortality on all broadleaf plants tested (Asclepias syriaca, Asclepias curassavica, Apocynum cannabinum, Monarda fistulosa, Rudbeckia hirta, PSW, BSW) with the exception of Glycine max, and had no effect on the monocots Schizachyrium scoparium and Zea mays. Although these laboratory studies indicate that most broadleaf vegetation may be susceptible to the pathogen, S. rolfsii might have potential as a mycoherbicide in natural eco-niche environments where invasive PSW and BSW have already become the predominant vegetation. Further laboratory testing of S. rolfsii and limited field testing at the initial discovery site are needed in order to prevent premature rejection of this isolate as a potential management tool against these highly invasive weeds. C1 [Gibson, Donna M.; Vaughan, Richard H.; Biazzo, Jeromy; Milbrath, Lindsey R.] ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Gibson, DM (reprint author), ARS, USDA, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. EM Donna.Gibson@ars.usda.gov FU Monroe County Parks Department, NY FX We gratefully acknowledge the support of the Monroe County Parks Department, NY, which have allowed us access to the discovery site for pursuing this research. Mention of a trademark, proprietary product, or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products or vendors that may also be suitable. NR 46 TC 3 Z9 3 U1 4 U2 21 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2014 VL 7 IS 2 BP 320 EP 327 DI 10.1614/IPSM-D-13-00086.1 PG 8 WC Plant Sciences SC Plant Sciences GA AK5PT UT WOS:000338479000013 ER PT J AU Wheeler, GS Ding, JQ AF Wheeler, Gregory S. Ding, Jianqing TI Is Chinese Tallowtree, Triadica sebifera, an Appropriate Target for Biological Control in the United States? SO INVASIVE PLANT SCIENCE AND MANAGEMENT LA English DT Article DE Bikasha collaris (Baly); classical biological control of weeds; integrated control; invasive species ID TREE SAPIUM-SEBIFERUM; INVASIVE PLANT; CONTROL AGENTS; MELALEUCA-QUINQUENERVIA; POPULATION-DYNAMICS; GENETIC-VARIATION; SOUTH-AFRICA; L ROXB; EUPHORBIACEAE; HERBIVORY AB Biological control is one of the most common approaches used to manage invasive weeds of wetlands and other natural areas. Before candidate agents can be released, research is conducted to support biological, control, which can be protracted and expensive, leading to a scientific and potentially lengthy regulatory review. To increase biological control safety, efficacy, and transparency, we suggest that during the early phases of a weed project, the feasibility of the invasive plant as a target should be studied explicitly. Our purpose here is to summarize information of an important invasive weed that can serve to judge whether the project is appropriate. Chinese tallowtree, Triadica sebifera, is one of the worst invasive species invading coastal wetlands and other riparian areas of the southeastern United States. Current management practices have not controlled the spread of this weed into these sensitive habitats. Initial surveys in the plant's native Chinese range for potential biological control agents have recovered several herbivore species that could be developed. These potential agents include defoliators, root and foliage feeders, and gall formers, whose biology, apparent host specificity, and impacts on plant fitness suggest that biological control offers great promise against Chinese tallowtree. When conducted during the initial phase of a project, this type of feasibility study can address potential conflicts of interest and risks, ultimately producing projects that are more effective and safer for biological control. C1 [Wheeler, Gregory S.] ARS, USDA, Invas Plant Res Lab, Ft Lauderdale, FL 33314 USA. Chinese Acad Sci, Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden Inst, Wuhan 430074, Hubei Province, Peoples R China. RP Wheeler, GS (reprint author), ARS, USDA, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM greg.wheeler@ars.usda.gov FU China National Basic Study Program [2012CB114104]; Florida Fish and Wildlife Conservation Commission; Florida Department of Environmental Protection [13084]; USDA/ARS FX We thank H. J. Esser, Botanische Staatssammlug, Munchen, Germany and P. E. Berry, University of Michigan, who provided invaluable advice on the phylogeny of the Euphorbiaceae for which we are exceedingly grateful. This research was supported by the China National Basic Study Program (2012CB114104 to JD), Florida Fish and Wildlife Conservation Commission, and Florida Department of Environmental Protection (Contract #13084 to GW) and USDA/ARS. NR 96 TC 3 Z9 3 U1 4 U2 18 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 1939-7291 EI 1939-747X J9 INVAS PLANT SCI MANA JI Invasive Plant Sci. Manag. PD APR-JUN PY 2014 VL 7 IS 2 BP 345 EP 359 DI 10.1614/IPSM-D-13-00061.1 PG 15 WC Plant Sciences SC Plant Sciences GA AK5PT UT WOS:000338479000016 ER PT J AU Yeh, HY Hiett, KL Line, JE Seal, BS AF Yeh, Hung-Yueh Hiett, Kelli L. Line, John E. Seal, Bruce S. TI Characterization and antigenicity of recombinant Campylobacter jejuni flagellar capping protein FliD SO JOURNAL OF MEDICAL MICROBIOLOGY LA English DT Article ID AEROMONAS-HYDROPHILA AL09-73; HOOK-ASSOCIATED PROTEINS; MATERNAL ANTIBODIES; SALMONELLA-TYPHIMURIUM; CLOSTRIDIUM-DIFFICILE; UNITED-STATES; CHICKENS; GLYCOSYLATION; COLONIZATION; POULTRY AB Campylobacter jejuni, a flagellated, spiral-rod, Gram-negative bacterium, is the leading pathogen of human acute bacterial gastroenteritis worldwide, and chickens are regarded as a major reservoir of this micro-organism. Bacterial flagella, composed of more than 35 proteins, play important roles in colonization and adhesion to the mucosal surface of chicken caeca. In this study, the flagellar capping protein, FliD, encoded by the fliD gene, from the Campylobacter jenuni D1-39 isolate was expressed and characterized, and its antigenicity determined. The fliD gene comprised 1929 nt, potentially encoding a 642 aa peptide with a calculated molecular mass of 69.6 kDa. This gene was PCR amplified and overexpressed in Escherichia coli. The recombinant FliD protein was purified by cobalt-chelating affinity chromatography and confirmed by nucleotide sequencing of the expression plasmid, SDS-PAGE analysis, His tag detection and matrix-assisted laser desorption/ionization time of flight mass spectrometry. The immunoblot data showed that the purified recombinant FliD protein reacted strongly to sera from broiler chickens older than 4 weeks, indicating that anti-FliD antibody may be prevalent in the poultry population. These results provide a rationale for further evaluation of the FliD protein as a vaccine candidate for broiler chickens to improve food safety for poultry. C1 [Yeh, Hung-Yueh; Hiett, Kelli L.; Line, John E.; Seal, Bruce S.] ARS, USDA, Richard B Russell Agr Res Ctr, Poultry Microbiol Safety Res Unit, Athens, GA 30605 USA. RP Yeh, HY (reprint author), ARS, USDA, Richard B Russell Agr Res Ctr, Poultry Microbiol Safety Res Unit, 950 Coll Stn Rd, Athens, GA 30605 USA. EM hungyueh.yeh@ars.usda.gov FU USDA Agricultural Research Service CRIS [6612-32000-060-00]; US Poultry & Egg Association [679] FX We are grateful to Susan Q. Brooks, Johnna.K. Garrish and Latoya T. Wiggins of Poultry Microbiological Safety Research Unit, Agricultural Research Service, US Department of Agriculture, Athens, GA, USA, for the excellent technical support. We also thank Dr Brian E. Scheffler and his Bioinformatics Group at the USDA AIRS Genomics and Bioinformatics Research Unit in Stoneville, MS, USA, for sequencing and bioinformatics, and Dr James A. Mobley and his team at the Bioanalytical and Mass Spectrometry Shared Facility of the University of Alabama at Birmingham, AL, USA, for MALDI-TOF analysis. We are grateful to Dr Holly Sellers of Poultry Diagnostic & Research Center of the University of Georgia, GA, USA, for chicken sera. This study was supported by the USDA Agricultural Research Service CRIS project no. 6612-32000-060-00, and the US Poultry & Egg Association project no. 679. Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The US Department of Agriculture is an equal opportunity provider and employer. NR 66 TC 8 Z9 8 U1 1 U2 3 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-2615 EI 1473-5644 J9 J MED MICROBIOL JI J. Med. Microbiol. PD APR PY 2014 VL 63 BP 602 EP 609 DI 10.1099/jmm.0.060095-0 PN 4 PG 8 WC Microbiology SC Microbiology GA AK5PP UT WOS:000338478600016 PM 24445509 ER PT J AU Asiimwe, P Naranjo, SE Ellsworth, PC AF Asiimwe, Peter Naranjo, Steven E. Ellsworth, Peter C. TI Effects of Irrigation Levels on Interactions Among Lygus hesperus (Hemiptera: Miridae), Insecticides, and Predators in Cotton SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Lygus hesperus; irrigation; arthropod predator; selective insecticide; cotton ID BOTTOM-UP FORCES; ORIUS-INSIDIOSUS HEMIPTERA; TOP-DOWN; NATURAL ENEMIES; RELATIVE ROLES; BEMISIA-TABACI; WATER-STRESS; INSECTS; POPULATIONS; HERBIVORE AB Variation in plant quality and natural enemy abundance plays an important role in insect population dynamics. In manipulative field studies, we evaluated the impact of varying irrigation levels and insecticide type on densities of Lygus hesperus Knight and the arthropod predator community in cotton. Three watering levels were established via irrigations timed according to three levels of percent soil water depletion (SWD): 20, 40, or 60, where 40% SWD is considered standard grower practice, 60% represents a deficit condition likely to impose plant productivity losses, and 20% represents surplus conditions with likely consequences on excessive vegetative plant production. The two key L. hesperus insecticides used were the broad-spectrum insecticide acephate and the selective insecticide flonicamid, along with an untreated check. We hypothesized that densities of L. hesperus and its associated predators would be elevated at higher irrigation levels and that insecticides would differentially impact L. hesperus and predator dynamics depending on their selectivity. L. hesperus were more abundant at the higher irrigation level (20% SWD) but the predator densities were unaffected by irrigation levels. Both L. hesperus and its predators were affected by the selectivity of the insecticide with highest L. hesperus densities and lowest predator abundance where the broad spectrum insecticide (acephate) was used. There were no direct interactions between irrigation level and insecticides, indicating that insecticide effects on L. hesperus and its predators were not influenced by the irrigation levels used here. The implications of these findings on the overall ecology of insect-plant dynamics and yield in cotton are discussed. C1 [Asiimwe, Peter; Ellsworth, Peter C.] Univ Arizona, Maricopa Agr Ctr, Dept Entomol, Maricopa, AZ 85138 USA. [Naranjo, Steven E.] ARS, USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Asiimwe, P (reprint author), Univ Arizona, Maricopa Agr Ctr, Dept Entomol, 37860 West Smith Enke Rd, Maricopa, AZ 85138 USA. EM peter.asiimwe@monsanto.com OI Ellsworth, Peter/0000-0002-2485-0830 FU Maricopa Agricultural Center; U.S. Department of Agriculture-Agricultural Research Service; U.S. Department of Agriculture-Risk Avoidance and Mitigation Program; Cotton Incorporated; AZ Cotton Growers Association; Cotton Foundations FX We thank Y. Carriere, G. Wang, R. Kaggwa-Asiimwe, and two anonymous reviewers for helpful comments on earlier drafts of this manuscript. E. Martin provided guidance on establishing the irrigation regimes. M. Stefanek, V. Barkley, G. Castro, F. Bojorquez, B. Stuart, J. Trejo, A. Slade, L. Rodarte, and A. Sonoqui provided expert field and laboratory technical assistance. We also appreciate the funding support of the Maricopa Agricultural Center, U.S. Department of Agriculture-Agricultural Research Service, and grants from the U.S. Department of Agriculture-Risk Avoidance and Mitigation Program, Cotton Incorporated, AZ Cotton Growers Association, and the Cotton Foundations. NR 65 TC 3 Z9 3 U1 1 U2 18 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 263 EP 273 DI 10.1603/EN12328 PG 11 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500002 PM 24533912 ER PT J AU Landolt, PJ Ohler, B Lo, P Cha, D Davis, TS Suckling, DM Brunner, J AF Landolt, Peter J. Ohler, Bonnie Lo, Peter Cha, Dong Davis, Thomas S. Suckling, David M. Brunner, Jay TI N-Butyl Sulfide as an Attractant and Coattractant for Male and Female Codling Moth (Lepidoptera: Tortricidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Cydia pomonella; codling moth; attractant; kairomone; N-butyl sulfide ID PLUS ACETIC-ACID; CYDIA-POMONELLA; KAIROMONE; APPLE; FRUIT; BAITS; ORCHARDS; TESTS; TRAP AB Research to discover and develop attractants for the codling moth, Cydia pomonella L., has involved identification of the chemicals eliciting moth orientation to conspecific female moths, host fruits, fermented baits, and species of microbes. Pear ester, acetic acid, and N-butyl sulfide are among those chemicals reported to attract or enhance attractiveness to codling moth. We evaluated the trapping of codling moth with N-butyl sulfide alone and in combination with acetic acid and pear ester in apple orchards. Acetic acid was attractive in two tests and N-butyl sulfide was attractive in one of two tests. N-Butyl sulfide increased catches of codling moth when used with acetic acid to bait traps. N-Butyl sulfide also increased catches of codling moth when added to traps baited with the combination of acetic acid and pear ester. Male and female codling moth both responded to these chemicals and chemical combinations. These results provide a new three-component lure comprising N-butyl sulfide, acetic acid, and pear ester that is stronger for luring codling moth females than other attractants tested. C1 [Landolt, Peter J.; Cha, Dong; Davis, Thomas S.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Ohler, Bonnie; Brunner, Jay] Washington State Univ, Tree Fruit Res & Extens Ctr, Wenatchee, WA 98801 USA. [Lo, Peter] Plant & Food Res, Havelock North 4130, Hawkes Bay, New Zealand. [Suckling, David M.] Plant & Food Res, Lincoln 7608, New Zealand. RP Landolt, PJ (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov RI Suckling, David/F-7005-2010 OI Suckling, David/0000-0001-7216-9348 FU Washington Tree Fruit Research Commission; Plant and Food Research New Zealand under their Semiochemicals for integrated pest management Project FX We are grateful to Jewel Brumley, Daryl Green, and Heather Headrick in Washington, and Kate Colhoun, Bernie Attfield, Z. Duncan, and Tom Sullivan in New Zealand for technical assistance. We thank Elwood Crozier and Obert Cold Storage Co., for permitting access to their orchards. This work was supported in part by funding from the Washington Tree Fruit Research Commission, and Plant and Food Research New Zealand under their Semiochemicals for integrated pest management Project. NR 24 TC 4 Z9 5 U1 1 U2 11 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 291 EP 297 DI 10.1603/EN13178 PG 7 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500005 PM 24534117 ER PT J AU Arp, A Munyaneza, JE Crosslin, JM Trumble, J Bextine, B AF Arp, Alex Munyaneza, Joseph E. Crosslin, James M. Trumble, John Bextine, Blake TI A Global Comparison of Bactericera cockerelli (Hemiptera: Triozidae) Microbial Communities SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE potato psyllid; Bactericera cockerelli; microbiome; Wolbachia; Candidatus Carsonella ruddii ID CANDIDATUS LIBERIBACTER SOLANACEARUM; ZEBRA CHIP DISEASE; REAL-TIME PCR; UNITED-STATES; POTATO; DIFFERENTIATION; ASSOCIATION; MANAGEMENT; DIVERSITY; SEQUENCE AB The potato psyllid (Bactericera cockerelli Sulc) is an economically important insect pest of solanaceous crops such as potato, tomato, pepper, and tobacco. Historically, the potato psyllid's range included central United States, Mexico, and California; more recently, populations of this insect have been reported in Central America, the Pacific Northwest, and New Zealand. Like most phytophagous insects, potato psyllids require symbiotic bacteria to compensate for nutritional deficiencies in their diet. Potato psyllids harbor the primary symbiont, Candidatus Carsonella ruddii, and may also harbor many secondary symbionts such as Wolbachia sp., Sodalis sp., Pseudomonas sp., and others. These secondary symbionts can have an effect on reproduction, nutrition, immune response, and resistances to heat or pesticides. To identify regional differences in potato psyllid bacterial symbionts, 454 pyrosequencing was performed using generic 16S rRNA gene primers. Analysis was performed using the Qiime 1.6.0 software suite, ARB Silva, and R. Operational taxonomic units were then grouped at 97% identity. Representative sequences were classified to genus using the ARB SILVA database. Potato psyllids collected in California contained a less diverse microbial community than those collected in the central United States and Central America. The crop variety, collection year, and haplotype did not seem to affect the microbial community in potato psyllids. The primary difference between psyllids in different regions was the presence and overall bacterial community composition of Candidatus Carsonella ruddii and Wolbachia. C1 [Arp, Alex] Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. [Munyaneza, Joseph E.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Crosslin, James M.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. [Trumble, John] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Bextine, Blake] Univ Texas Tyler, Dept Biol, Tyler, TX 75799 USA. RP Arp, A (reprint author), Univ Florida, Citrus Res & Educ Ctr, Dept Entomol & Nematol, Lake Alfred, FL 33850 USA. EM aarp@ufl.edu FU U.S. Department of Agriculture-Risk Avoidance and Mitigation Program (USDA-RAMP) [2009-51101-05892]; U.S. Department of Agriculture-Specialty Crop Research Initiative (USDA-SCRI) [2009-51181-20178] FX We are grateful to Venkat Sengoda, Launa Hamlin, and Casey Butler for technical assistance. We also thank Anne Nissinen for providing the carrot psyllid samples. This research was partially funded by U.S. Department of Agriculture-Risk Avoidance and Mitigation Program (USDA-RAMP Project; 2009-51101-05892) and U.S. Department of Agriculture-Specialty Crop Research Initiative (USDA-SCRI Project; 2009-51181-20178). NR 36 TC 5 Z9 6 U1 1 U2 16 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 344 EP 352 DI 10.1603/EN13256 PG 9 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500011 PM 24517908 ER PT J AU Swezey, SL Nieto, DJ Pickett, CH Hagler, JR Bryer, JA Machtley, SA AF Swezey, Sean L. Nieto, Diego J. Pickett, Charles H. Hagler, James R. Bryer, Janet A. Machtley, Scott A. TI Spatial Density and Movement of the Lygus spp. Parasitoid Peristenus relictus (Hymenoptera: Braconidae) in Organic Strawberries With Alfalfa Trap Crops SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE California; strawberry; Lygus hesperus; alfalfa trap crop; ELISA ID HEMIPTERA-MIRIDAE; PLANT BUG; TIME ALLOCATION; CALIFORNIA; MARKING; SEMIOCHEMICALS; ESTABLISHMENT; AGGREGATION; POPULATIONS; DIGONEUTIS AB Alfalfa trap crops are currently used to manage Lygus spp. in organic strawberry fields on the California Central Coast. The retention of Lygus spp. in alfalfa creates aggregated distributions that provide improved opportunities for biological control by the introduced parasitoid Peristenus relictus (Ruthe). The abundance and distribution of P. relictus between two trap crops separated by 50 strawberry rows were analyzed in 2008 and 2010. Parasitism of Lygus spp. nymphs by P. relictus (measured by larval abundance and % parasitism) was greatest in alfalfa trap crops compared with strawberry rows. A significantly positive correlation between host nymphs and P. relictus larvae in and between trap crops was found. Movement of P. relictus adults from a marked alfalfa trap crop into adjacent strawberry rows or trap crops was also studied in 2008 and 2009 using a chicken egg-albumin enzyme-linked immunosorbent assay mark-capture technique. In 2008 and 2009, 85 and 49% of protein-marked wasps were captured from central trap crops, respectively, indicating that alfalfa trap crops act as a concentrated "host-density anchor" in organic strawberry fields. C1 [Swezey, Sean L.; Nieto, Diego J.; Bryer, Janet A.] Univ Calif Santa Cruz, Ctr Agroecol & Sustainable Food Syst, Santa Cruz, CA 95064 USA. [Pickett, Charles H.] Calif Dept Food & Agr, Biol Control Program, Sacramento, CA 95832 USA. [Hagler, James R.; Machtley, Scott A.] USDA, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Swezey, SL (reprint author), Univ Calif Santa Cruz, Ctr Agroecol & Sustainable Food Syst, 1156 High St, Santa Cruz, CA 95064 USA. EM findit@ucsc.edu FU United States Department of Agriculture (USDA) Cooperative State Research, Education, and Extension Service Pest Management Alternatives Program FX We thank Larry Eddings, Joe Valdez, and Ramon Serrano of Pacific Gold Farms, Moss Landing, CA, for their cooperation and management of organic strawberry production areas. Without their patience and cooperation, this project would not have been possible. We also thank Neal Murray, Clayton Winter, Andre Burnier, Caitlin Slay, Felisa Blackmer, Susan Fountain, and Marypat Stadtherr for excellent technical support. We thank Dale Spurgeon, Steven Naranjo, and Martha Brown for their reviews of this paper. This project was funded, in part, by the United States Department of Agriculture (USDA) Cooperative State Research, Education, and Extension Service Pest Management Alternatives Program. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. The USDA is an equal-opportunity provider and employer. NR 31 TC 7 Z9 7 U1 4 U2 28 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 363 EP 369 DI 10.1603/EN13128 PG 7 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500013 PM 24763093 ER PT J AU Tillman, PG Carpenter, JE AF Tillman, P. G. Carpenter, J. E. TI Milkweed (Gentianales: Apocynaceae): A Farmscape Resource for Increasing Parasitism of Stink Bugs (Hemiptera: Pentatomidae) and Providing Nectar to Insect Pollinators and Monarch Butterflies SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Trichopoda pennipes; Nezara viridula; Chinavia hilaris; nectar provision ID NEZARA-VIRIDULA; HETEROPTERA-PENTATOMIDAE; HABITAT MANAGEMENT; TACHINIDAE DIPTERA; BIOLOGICAL-CONTROL; FLOWERING PLANTS; COTTON; LEPIDOPTERA; LONGEVITY; FIELD AB In peanut-cotton farmscapes in Georgia, the stink bugs Nezara viridula (L.) and Chinavia hilaris (Say) (Hemiptera: Pentatomidae) and the leaffooted bug, Leptoglossus phyllopus (L.) (Hemiptera: Coreidae), disperse at crop-to-crop interfaces to feed on bolls in cotton. The main objective of this study was to determine whether insecticide-free tropical milkweed (Asclepias curassavica L.), a nectar-producing plant, can increase parasitism of these bugs by Trichopoda pennipes (F.) (Diptera: Tachinidae) and provide nectar to monarch butterflies and insect pollinators in these farmscapes. Peanut-cotton plots with and without flowering milkweed plants were established in 2009 and 2010. Adult T. pennipes, monarch butterflies, honey bees, and native insect pollinators readily fed on floral nectar of milkweed. Monarch larvae feeding on milkweed vegetation successfully developed into pupae. In 2009, N. viridula was the primary host of T. pennipes in cotton, and parasitism of this pest by the parasitoid was significantly higher in milkweed cotton (61.6%) than in control cotton (13.3%). In 2010, parasitism of N. viridula, C. hilaris, and L. phyllopus by T. pennipes was signi_cantly higher in milkweed cotton (24.0%) than in control cotton (1.1%). For both years of the study, these treatment differences were not owing to a response by the parasitoid to differences in host density, because density of hosts was not significantly different between treatments. In conclusion, incorporation of milkweed in peanut-cotton plots increased stink bug parasitism in cotton and provided nectar to insect pollinators and monarch butterflies. C1 [Tillman, P. G.; Carpenter, J. E.] ARS, Crop Protect & Management Res Lab, USDA, Tifton, GA 31793 USA. RP Tillman, PG (reprint author), ARS, Crop Protect & Management Res Lab, USDA, POB 748, Tifton, GA 31793 USA. EM glynn.tillman@ars.usda.gov FU National Center for Appropriate Technology FX We thank Kristie Graham and Brittany Giles for technical assistance in the field. Funding was provided, in part, by the National Center for Appropriate Technology. NR 47 TC 3 Z9 4 U1 6 U2 55 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 370 EP 376 DI 10.1603/EN13175 PG 7 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500014 PM 24763094 ER PT J AU Kleiber, JR Unelius, CR Lee, JC Suckling, DM Qian, MC Bruck, DJ AF Kleiber, Joseph R. Unelius, C. Rikard Lee, Jana C. Suckling, David Maxwell Qian, Michael C. Bruck, Denny J. TI Attractiveness of Fermentation and Related Products to Spotted Wing Drosophila (Diptera: Drosophilidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE apple cider vinegar; bait; monitoring; spotted wing drosophila; trapping ID SUZUKII; VINEGAR; IDENTIFICATION; MELANOGASTER; INFESTATION; RASPBERRIES; VOLATILES; CHERRIES; YEASTS AB Laboratory screening bioassays and field trapping experiments of spotted wing drosophila flies, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), were conducted to determine the attractiveness of 17 compounds as well as to compare attractant efficiency during peak fruit ripeness and postharvest captures late in the season. Compounds structurally related to each of the fermentation products acetic acid, ethanol, ethyl acetate, and 2-phenethyl alcohol were screened for attractiveness compared with a soap water control in greenhouse cage bioassays. The compounds determined to be attractive in the greenhouse bioassay (methanol, ethanol, propanol, formic acid, acetic acid, ethyl acetate, propyl acetate, phenethyl acetate, phenethyl propionate, and phenethyl butyrate) were individually tested in the field added to apple cider vinegar (ACV). The acids were also tested individually in neutralized ACV (NACV; pH approximate to 7). Combinations of the compounds were tested in NACV. The capture numbers in ACV traps were not significantly increased by the addition of any of the compounds tested, although significant deterrent effects of some of the compounds allowed differences between treatments to be observed. Compounds that are most prevalent in wine and vinegar (methanol, ethanol, acetic acid, and ethyl acetate) as well as phenethyl propionate and phenethyl butyrate were less deterrent than the other compounds tested in the field. Captures during peak fruit ripeness were compared with the postharvest period when fruit hosts were not available or were overripe. Although the total number of flies captured late in the season was lower, the trends in treatment performance were similar, indicating a consistent performance of these baits from peak fruit ripeness through postharvest. C1 [Kleiber, Joseph R.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Unelius, C. Rikard; Suckling, David Maxwell] New Zealand Inst Plant & Food Res Ltd, Christchurch 8140, New Zealand. [Unelius, C. Rikard] Linnaeus Univ, Dept Chem & Biomed Sci, SE-39182 Kalmar, Sweden. [Lee, Jana C.] ARS, Hort Crops Res Unit, USDA, Corvallis, OR 97331 USA. [Qian, Michael C.] Oregon State Univ, Corvallis, OR 97331 USA. [Bruck, Denny J.] DuPont Pioneer, Johnston, IA 50131 USA. RP Lee, JC (reprint author), ARS, Hort Crops Res Unit, USDA, 3420 Orchard Ave, Corvallis, OR 97331 USA. EM jana.lee@ars.usda.gov RI Unelius, Carl Rikard/B-3183-2008; Suckling, David/F-7005-2010 OI Unelius, Carl Rikard/0000-0001-7158-6393; Suckling, David/0000-0001-7216-9348 FU U.S. Department of Agriculture-Current Research Information System [5358-22000-037-00]; Specialty Crop Research Initiative [2010-51181-21167]; Better Border Biosecurity FX We thank Adam Cave, Kelly Donahue, and Amanda Lake for assistance in conducting greenhouse bioassays and Jimmy Klick and Scott Shane for assistance in field trapping. This study was supported by U.S. Department of Agriculture-Current Research Information System 5358-22000-037-00 and Specialty Crop Research Initiative 2010-51181-21167 and Better Border Biosecurity (www.b3nz.org). NR 31 TC 9 Z9 9 U1 4 U2 59 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 439 EP 447 DI 10.1603/EN13224 PG 9 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500022 PM 24763098 ER PT J AU Lightle, DM Quito-Avila, D Martin, RR Lee, JC AF Lightle, D. M. Quito-Avila, D. Martin, R. R. Lee, J. C. TI Seasonal Phenology of Amphorophora agathonica (Hemiptera: Aphididae) and Spread of Viruses in Red Raspberry in Washington SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE closteroviridae; reoviridae; RpLV; RLMV; raspberry aphid AB Amphorophora agathonica (Hottes) is the primary vector of aphid-transmitted viruses in red raspberry in the Pacific Northwest region of the United States. To better understand the biology of the aphid, we estimated the lower developmental threshold and studied the seasonal activity of A. agathonica in commercial fields in northern Washington state. In addition, we monitored the spread of raspberry viruses (raspberry latent virus and raspberry leaf mottle virus, RLMV) to determine how rapidly fields became infected and whether there was a relationship between aphid presence and infection. The lower developmental threshold of A. agathonica was estimated to be 2.7 degrees C. In the field, apterous and alate aphid populations began rapidly increasing at approximate to 800 growing degree-days and peaked at 1,050 growing degree-days. RLMV spread rapidly, with 30-60% of plants in four different commercial fields testing positive after three growing seasons. There was no discernible relationship between the presence or abundance of aphids based on 10 leaves sampled per plant location, and the odds of that plant becoming infected with RLMV. C1 [Lightle, D. M.] Oregon State Univ, Entomol Program, Corvallis, OR 97331 USA. [Lightle, D. M.; Martin, R. R.; Lee, J. C.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97331 USA. [Quito-Avila, D.] Escuela Super Politecn Litoral, ESPOL, CIBE, Dept Plant Pathol, Guayaquil, Ecuador. RP Lightle, DM (reprint author), Oregon State Univ, Entomol Program, 4017 Ag & Life Sci Bldg, Corvallis, OR 97331 USA. EM lightled@onid.orst.edu FU U.S. Department of Agriculture (USDA) Specialty Crops Research Initiative grant [2009-51181-06022]; Western Sustainable Agriculture Research and Education [GW12-022]; USDA Current Research Information System (CRIS) [5358-22000-032-00D] FX We thank G. Moulton for field collections; C. Fieland, D. Hicks, J. Mindolovich, and J. Wong for processing field samples; N. Mosier and K. Sarver for assistance with virus testing; and L. Coop, G. Fisher, and two anonymous reviewers for comments on an earlier draft of this article. Funding was provided by a U.S. Department of Agriculture (USDA) Specialty Crops Research Initiative grant 2009-51181-06022, Western Sustainable Agriculture Research and Education GW12-022, and USDA Current Research Information System (CRIS) 5358-22000-032-00D. NR 13 TC 0 Z9 0 U1 1 U2 9 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 467 EP 473 DI 10.1603/EN13213 PG 7 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500025 PM 24763099 ER PT J AU Fuentes-Contreras, E Basoalto, E Franck, P Lavandero, B Knight, AL Ramirez, CC AF Fuentes-Contreras, Eduardo Basoalto, Esteban Franck, Pierre Lavandero, Blas Knight, Alan L. Ramirez, Claudio C. TI Measuring Local Genetic Variability in Populations of Codling Moth (Lepidoptera: Tortricidae) Across an Unmanaged and Commercial Orchard Interface SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Cydia pomonella; genetic structure; microsatellite; Bayesian assignment; TESS ID POMONELLA L. LEPIDOPTERA; CYDIA-POMONELLA; CENTRAL CHILE; APPLE ORCHARDS; LANDSCAPE GENETICS; AZINPHOS-METHYL; PEAR ESTER; DISPERSAL; AGROECOSYSTEMS; RESISTANCE AB The genetic structure of adult codling moth, Cydia pomonella (L.), populations was characterized both inside a managed apple, Malus domestica Borkdhausen, orchard and in surrounding unmanaged hosts and nonhost trees in central Chile during 2006-2007. Adult males were collected using an array of sex pheromone-baited traps. Five microsatellite genetic markers were used to study the population genetic structure across both spatial (1-100 ha) and temporal (generations within a season) gradients. Analysis of molecular variance (AMOVA) found a significant, but weak, association in both the spatial and temporal genetic structures. Discriminant analysis also found signi_cant differentiation between the first and second generation for traps located either inside or outside the managed orchard. The Bayesian assignment test detected three genetic clusters during each of the two generations, which corresponded to different areas within the unmanaged and managed apple orchard interface. The lack of a strong spatial structure at a local scale was hypothesized to be because of active adult movement between the managed and unmanaged hosts and the asymmetry in the insecticide selection pressure inside and outside the managed habitats. These data highlight the importance of developing area-wide management programs that incorporate management tactics effective at the landscape level for successful codling moth control. C1 [Fuentes-Contreras, Eduardo] Univ Talca, Fac Ciencias Agr, Dept Prod Agr, Talca, Chile. [Basoalto, Esteban] Oregon State Univ, Southern Oregon Res & Extens Ctr, Central Point, OR 97502 USA. [Franck, Pierre] INRA, Plantes & Syst Culture Horticoles UR115, F-84914 Avignon 9, France. [Lavandero, Blas; Ramirez, Claudio C.] Univ Talca, Inst Biol Vegetal & Biotecnol, Lab Interacc Insectoplanta, Talca, Chile. [Knight, Alan L.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Fuentes-Contreras, E (reprint author), Univ Talca, Fac Ciencias Agr, Dept Prod Agr, Casilla 747, Talca, Chile. EM efuentes@utalca.cl RI Fuentes-Contreras, Eduardo/A-7595-2008; Ramirez, Claudio/A-7176-2008; Basoalto Venegas, Esteban/A-6384-2016; OI Fuentes-Contreras, Eduardo/0000-0003-3567-3830; Ramirez, Claudio/0000-0002-6110-3973; Basoalto Venegas, Esteban/0000-0003-2280-8978; Franck, Pierre/0000-0002-1904-8325 FU FONDECYT [1071029]; Universidad de Talca FX This work was funded by FONDECYT grant 1071029 to E.F.C.A doctoral fellowship to E.B. from Universidad de Talca is also acknowledged. Tom Unruh, U.S. Department of Agriculture-Agricultural Research Service, Wapato, WA, and John T. Margaritopoulos, Thessaly University, Greece, kindly provided a review of an early version of the manuscript. NR 49 TC 2 Z9 2 U1 1 U2 25 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD APR PY 2014 VL 43 IS 2 BP 520 EP 527 DI 10.1603/EN13131 PG 8 WC Entomology SC Entomology GA AJ5BC UT WOS:000337694500031 PM 24763103 ER PT J AU Maldonado, A Youssef, R McDonald, M Brewer, E Beard, H Matthews, B AF Maldonado, Andrea Youssef, Reham McDonald, Margaret Brewer, Eric Beard, Hunter Matthews, Benjamin TI Overexpression of four Arabidopsis thaliana NHL genes in soybean (Glycine max) roots and their effect on resistance to the soybean cyst nematode (Heterodera glycines) SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Defense response; NHL; NDR1; Resistance; Soybean cyst nematode; Transgenic root ID UBIQUITIN LIGASES; SALICYLIC-ACID; PLANT DEFENSE; PCR DATA; EXPRESSION; INFECTION; JASMONATE; PROTEIN; NDR1; SUSCEPTIBILITY AB Glycine max is one of the most important cash-crops in the US. Major losses in soybean production are reported annually due to the attack of the soybean cyst nematode (Heterodera glycines). Since chemical control is neither environmental nor economically viable, efforts have been focused on enhancing the plants defense system. Plants have developed mechanisms to identify and react to pathogen attack. Basal defenses are activated by resistance (R) proteins. The Arabidopsis NON-RACE-SPECIFIC DISEASE RESISTANCE 1, NDR1 protein shows some sequence similarity to the tobacco HAIRPIN INDUCED GENE 1 (HINT), is localized to the plasma membrane, and usually interacts with CC-NB-LRR R proteins to mediate defense signaling. Forty five NDR1/HIN1-LIKE (NHL) genes have been found in Arabidopsis. Four of them were overexpressed in soybean roots to determine if they could confer resistance to H. glycines. AtNHL1 and AtNHL8 significantly decreased the number of mature females to 50 and 46% of the number found in control roots. Relative expression analysis showed they induced the expression of marker genes for jasmonic acid (PR3 and OSM, encoding chitinase B and an osmotin, respectively) and ethylene (EBF, ERF1, and ERS2, encoding the EIN3-binding F box protein, the ethylene response factor 1, and the ethylene response sensor 2, respectively). NHL1 is most similar to NDR1, and NHL8 is an E3 ubiquitin ligase, Ariadne ring zinc finger protein. Our observations confirm the role of NHL1 and NHL8 in the plant defense mechanism against pathogens observed in Arabidopsis, by conferring resistance to SCN in soybean. Published by Elsevier Ltd. C1 [Maldonado, Andrea; Youssef, Reham; McDonald, Margaret; Brewer, Eric; Beard, Hunter; Matthews, Benjamin] ARS, USDA, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Youssef, Reham] Fayoum Univ, Fac Agr, Dept Plant Protect, Al Fayyum, Egypt. RP Matthews, B (reprint author), ARS, USDA, Soybean Genom & Improvement Lab, 10300 Baltimore Ave,Bldg 006, Beltsville, MD 20705 USA. EM ben.matthews@ars.usda.gov FU United Soybean Board FX The authors thank Patrick Elia for DNA sequencing. Financial support from United Soybean Board is gratefully acknowledged. Mention of trade name, proprietary product or vendor does not constitute a guarantee or warranty of the product by the U.S. Department of Agriculture or imply its approval to the exclusion of other products or vendors that also may be suitable. The authors have no conflict of interest. NR 41 TC 4 Z9 4 U1 2 U2 19 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2014 VL 86 BP 1 EP 10 DI 10.1016/j.pmpp.2014.02.001 PG 10 WC Plant Sciences SC Plant Sciences GA AJ8XQ UT WOS:000337991800002 ER PT J AU Cassone, BJ Chen, ZB Chiera, J Stewart, LR Redinbaugh, MG AF Cassone, Bryan J. Chen, Zhenbang Chiera, Joseph Stewart, Lucy R. Redinbaugh, Margaret G. TI Responses of highly resistant and susceptible maize to vascular puncture inoculation with Maize dwarf mosaic virus SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Maize; Potyvirus; Gene expression; Resistance; Microarrays ID GENE-EXPRESSION PROFILE; LINES CARRYING WSM1; TOBACCO ETCH VIRUS; ARABIDOPSIS-THALIANA; SCMV RESISTANCE; RICE PLANTS; MECHANICAL TRANSMISSION; TRANSCRIPTION FACTORS; DISTANCE MOVEMENT; VIRAL-INFECTION AB Mechanisms for genetic resistance to diseases caused by potyviruses, especially Maize dwarf mosaic virus (MDMV), in maize (Zea mays L.) remain unclear. Previous studies indicated that MDMV can replicate in and spread to groups of cells in maize leaves of the virus resistant inbred line Pa405, but systemic movement of the virus did not occur. In this study, the responses of Pa405 and the virus-susceptible inbred line Oh28 were compared in germinating maize embryos. Four days post inoculation (dpi), MDMV was detected immunocytochemically in 45% of resistant and 19% of susceptible shoots, respectively. Systemic movement of MDMV out of inoculated leaves occurred between 2 and 4 dpi in Oh28 seedlings. Microarray analysis was used to examine the effects of MDMV inoculation on gene expression in shoots of resistant and susceptible lines at 4 dpi. Approximately 15% of transcripts were differentially expressed between the resistant and susceptible lines. Of the transcripts with >10 fold greater expression in one line, more than 70% were up-regulated in the resistant line. Substantially fewer genes were differentially expressed in MDMV- and mock-inoculated shoots, with 21 and 30 genes being differentially regulated in the susceptible and resistant lines, respectively. For a subset of genes, differential expression was validated using quantitative real-time PCR. There was no clustering of differentially expressed transcripts in the previously identified QTLs important for MDMV resistance. These results provide a short list of virus responsive transcripts to begin to explain the resistance response in Pa405 versus the susceptible response in Oh28. Published by Elsevier Ltd. C1 [Cassone, Bryan J.; Chiera, Joseph; Stewart, Lucy R.; Redinbaugh, Margaret G.] Ohio Agr Res & Dev Ctr, USDA, ARS Corn Soybean & Wheat Qual Res, Wooster, OH 44691 USA. [Chen, Zhenbang; Stewart, Lucy R.; Redinbaugh, Margaret G.] Ohio State Univ, Dept Plant Pathol, OARDC, Wooster, OH 44691 USA. RP Redinbaugh, MG (reprint author), Ohio Agr Res & Dev Ctr, USDA, ARS Corn Soybean & Wheat Qual Res, Wooster, OH 44691 USA. EM peg.redinbaugh@ars.usda.gov RI Redinbaugh, Margaret/A-3611-2013 NR 69 TC 3 Z9 3 U1 1 U2 11 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2014 VL 86 BP 19 EP 27 DI 10.1016/j.pmpp.2014.01.002 PG 9 WC Plant Sciences SC Plant Sciences GA AJ8XQ UT WOS:000337991800004 ER PT J AU Baker, CJ Mock, NM Whitaker, BD Hammond, RW Nemchinov, L Roberts, DP Aver'yanov, AA AF Baker, C. Jacyn Mock, Norton M. Whitaker, Bruce D. Hammond, Rose W. Nemchinov, Lev Roberts, Daniel P. Aver'yanov, Andrey A. TI Characterization of apoplast phenolics: In vitro oxidation of acetosyringone results in a rapid and prolonged increase in the redox potential SO PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY LA English DT Article DE Apoplast; Phenolic; Bacteria; Redox potential; Hydrogen peroxide; Oxidative burst ID SUSPENSION CELLS; PEROXIDASE; STRESS; BURST AB Previous work demonstrated that when tobacco cell suspensions were inoculated with certain bacterial strains, the redox potential of the suspensions would increase (oxidative), as much as 100 mV, and in some cases last more than an hour. To discover possible contributors to this prolonged redox potential burst, we examined the oxidation of various plant phenolics that were similar to those found in the cell suspensions. Acetosyringone, one of the major extracellular phenolics in tobacco cell suspensions, was unique in causing a prolonged increase in redox potential of more than 100 mV when incubated with H2O2 and peroxidase. The increase in potential appears to be due to a relatively stable radical intermediate. The length of time that the redox potential increased was dependent on the ratio of H2O2 and acetosyringone. The most favorable was a 1:1 ratio. In vitro characterization showed that production of this intermediate is favored by relatively low pH (around 6) and cooler temperatures possibly due to slower kinetics. Phosphate buffer was substituted for MES buffer, which had been used initially but was found to interfere with the oxidation. The results of this study demonstrate that apoplastic phenolics, in addition to often playing a role as antioxidants that reduce H2O2, can also cause rapid transient periods of extreme redox potential creating a local environment hostile to pathogen ingress. Published by Elsevier Ltd. C1 [Baker, C. Jacyn; Mock, Norton M.; Hammond, Rose W.; Nemchinov, Lev] USDA, Mol Plant Pathol Lab, Beltsville, MD 20705 USA. [Whitaker, Bruce D.] USDA, Produce Qual & Safety Lab, Beltsville, MD 20705 USA. [Roberts, Daniel P.] USDA, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Aver'yanov, Andrey A.] Res Inst Phytopathol, B Vyazemy 143050, Moscow Region, Russia. RP Baker, CJ (reprint author), USDA, Mol Plant Pathol Lab, Bldg 004 Rm 119, Beltsville, MD 20705 USA. EM jacyn.baker@ars.usda.gov NR 23 TC 4 Z9 4 U1 0 U2 7 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0885-5765 J9 PHYSIOL MOL PLANT P JI Physiol. Mol. Plant Pathol. PD APR PY 2014 VL 86 BP 57 EP 63 DI 10.1016/j.pmpp.2014.03.004 PG 7 WC Plant Sciences SC Plant Sciences GA AJ8XQ UT WOS:000337991800009 ER PT J AU Thornber, PM Rubira, RJ Styles, DK AF Thornber, P. M. Rubira, R. J. Styles, D. K. TI Humane killing of animals for disease control purposes SO REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES LA English DT Article DE Animal welfare; Depopulation; Disease control; Euthanasia; Killing; Novel technique ID MASS EMERGENCY DEPOPULATION; POULTRY OPERATIONS; CARBON-DIOXIDE; ON-FARM; FOAM; EUTHANASIA; SLAUGHTER; SWINE AB Killing for disease control purposes is an emotional issue for everyone concerned. Large-scale euthanasia or depopulation of animals may be necessary for the emergency control or eradication of animal diseases, to remove animals from a compromised situation (e.g. following flood, storm, fire, drought or a feed contamination event), to effect welfare depopulation when there is an oversupply due to a dysfunctional or closed marketing channel, or to depopulate and dispose of animals with minimal handling to decrease the risk of a zoonotic disease infecting humans. The World Organisation for Animal Health (OIE) developed international standards to provide advice on humane killing for various species and situations. Some fundamental issues are defined, such as competency of animal handling and implementation of humane killing techniques. Some of these methods have been used for many years, but novel approaches for the mass killing of particular species are being explored. Novel vaccines and new diagnostic techniques that differentiate between vaccinated and infected animals will save many animals from being killed as part of biosecurity response measures. Unfortunately, the destruction of affected livestock will still be required to control diseases whilst vaccination programmes are activated or where effective vaccines are not available. This paper reviews the principles of humane destruction and depopulation and explores available techniques with their associated advantages and disadvantages. It also identifies some current issues that merit consideration, such as legislative conflicts (emergency disease legislation versus animal welfare legislation, occupational health and safety), media issues, opinions on the future approaches to killing for disease control, and animal welfare. C1 [Thornber, P. M.; Rubira, R. J.] Dept Agr, Canberra, ACT 2601, Australia. [Styles, D. K.] Anim & Plant Hlth Inspect Serv, USDA, Vet Serv, Riverdale, MD 20737 USA. RP Thornber, PM (reprint author), Dept Agr, GPO Box 858, Canberra, ACT 2601, Australia. EM peter.thornber@daff.gov.au NR 18 TC 1 Z9 1 U1 7 U2 18 PU OFFICE INT EPIZOOTIES PI PARIS PA 12 RUE DE PRONY, 75017 PARIS, FRANCE SN 0253-1933 J9 REV SCI TECH OIE JI Rev. Sci. Tech. Off. Int. Epizoot. PD APR PY 2014 VL 33 IS 1 BP 303 EP 310 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA AJ5YH UT WOS:000337765700031 PM 25000803 ER PT J AU Nemec, K AF Nemec, Kristine TI Eleven New Records of Spiders (Arachnida, Araneae) for Nebraska SO JOURNAL OF THE KANSAS ENTOMOLOGICAL SOCIETY LA English DT Editorial Material ID SPECIES RICHNESS; CONSUMPTION; PESTS; LIST; CROP C1 [Nemec, Kristine] Univ Nebraska, Nebraska Cooperat Fish & Wildlife Res Unit, Lincoln, NE 68583 USA. RP Nemec, K (reprint author), USDA, North Cent Agr Res Lab, 2923 Medary Ave, Brookings, SD 57006 USA. EM kristinenemec14@gmail.com NR 45 TC 0 Z9 0 U1 1 U2 1 PU KANSAS ENTOMOLOGICAL SOC PI LAWRENCE PA PO BOX 368, LAWRENCE, KS 66044 USA SN 0022-8567 EI 1937-2353 J9 J KANSAS ENTOMOL SOC JI J. Kans. Entomol. Soc. PD APR PY 2014 VL 87 IS 2 BP 249 EP 252 PG 4 WC Entomology SC Entomology GA AJ4PJ UT WOS:000337658000008 ER PT J AU Henry, IM Nagalakshmi, U Lieberman, MC Ngo, KJ Krasileva, KV Vasquez-Gross, H Akhunova, A Akhunov, E Dubcovsky, J Tai, TH Comai, L AF Henry, Isabelle M. Nagalakshmi, Ugrappa Lieberman, Meric C. Ngo, Kathie J. Krasileva, Ksenia V. Vasquez-Gross, Hans Akhunova, Alina Akhunov, Eduard Dubcovsky, Jorge Tai, Thomas H. Comai, Luca TI Efficient Genome-Wide Detection and Cataloging of EMS-Induced Mutations Using Exome Capture and Next-Generation Sequencing SO PLANT CELL LA English DT Article ID CHEMICALLY-INDUCED MUTATIONS; TARGET ENRICHMENT; DISCOVERY; RICE; DNA; POPULATION; MUTANT; GENE; IDENTIFICATION; ARABIDOPSIS AB Chemical mutagenesis efficiently generates phenotypic variation in otherwise homogeneous genetic backgrounds, enabling functional analysis of genes. Advances in mutation detection have brought the utility of induced mutant populations on par with those produced by insertional mutagenesis, but systematic cataloguing of mutations would further increase their utility. We examined the suitability of multiplexed global exome capture and sequencing coupled with custom-developed bioinformatics tools to identify mutations in well-characterized mutant populations of rice (Oryza sativa) and wheat (Triticum aestivum). In rice, we identified similar to 18,000 induced mutations from 72 independent M2 individuals. Functional evaluation indicated the recovery of potentially deleterious mutations for > 2600 genes. We further observed that specific sequence and cytosine methylation patterns surrounding the targeted guanine residues strongly affect their probability to be alkylated by ethyl methanesulfonate. Application of these methods to six independent M2 lines of tetraploid wheat demonstrated that our bioinformatics pipeline is applicable to polyploids. In conclusion, we provide a method for developing large-scale induced mutation resources with relatively small investments that is applicable to resource-poor organisms. Furthermore, our results demonstrate that large libraries of sequenced mutations can be readily generated, providing enhanced opportunities to study gene function and assess the effect of sequence and chromatin context on mutations. C1 [Henry, Isabelle M.; Nagalakshmi, Ugrappa; Lieberman, Meric C.; Ngo, Kathie J.; Comai, Luca] Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. [Henry, Isabelle M.; Nagalakshmi, Ugrappa; Lieberman, Meric C.; Ngo, Kathie J.; Comai, Luca] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA. [Krasileva, Ksenia V.; Vasquez-Gross, Hans; Dubcovsky, Jorge] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Akhunova, Alina; Akhunov, Eduard] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66502 USA. [Akhunova, Alina] Kansas State Univ, Integrated Genom Facil, Manhattan, KS 66502 USA. [Dubcovsky, Jorge] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA. [Tai, Thomas H.] ARS, Crops Pathol & Genet Res Unit, USDA, Davis, CA USA. RP Comai, L (reprint author), Univ Calif Davis, Dept Plant Biol, Davis, CA 95616 USA. EM lcomai@ucdavis.edu RI Dubcovsky, Jorge/A-4969-2008; OI Dubcovsky, Jorge/0000-0002-7571-4345; Henry, Isabelle/0000-0002-6796-1119 FU National Science Foundation Plant Genome Award, TPGRP [DBI-0822383]; USDA-Agricultural Research Service CRIS Project [5306-21000-017/021-00D]; International Atomic Energy Agency [58-5306-0-112F]; California Rice Research Board RB-3 Project; Howard Hughes Medical Institute; Gordon and Betty Moore Foundation; USDA AFRI Triticeae CAP project [2011-68002-30029]; USDA NIFA postdoctoral fellowship [2012-67012-19811] FX We thank Helen T. Tsai for technical support. We thank the UC Davis Genome Center DNA Technologies Core for sequencing support. This work was supported by the National Science Foundation Plant Genome Award DBI-0822383, TPGRP, "Efficient identification of induced mutations in crop species by ultra-high-throughput DNA sequencing" (to L.C. and J.D.), the USDA-Agricultural Research Service CRIS Project 5306-21000-017/021-00D (to T.H.T.), the International Atomic Energy Agency 58-5306-0-112F (to T.H.T.), and the California Rice Research Board RB-3 Project (to T.H.T.). The rice genome and annotation information was made available by the "Rice Genome Annotation Project" (Kawahara et al., 2013). J.D. acknowledges support from the Howard Hughes Medical Institute and the Gordon and Betty Moore Foundation. E.A. and J.D. acknowledge support from the USDA AFRI Triticeae CAP project (2011-68002-30029). K.V.K. was supported by USDA NIFA postdoctoral fellowship grant number 2012-67012-19811. NR 41 TC 40 Z9 41 U1 8 U2 64 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD APR PY 2014 VL 26 IS 4 BP 1382 EP 1397 DI 10.1105/tpc.113.121590 PG 16 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA AJ0NO UT WOS:000337351300004 PM 24728647 ER PT J AU Gao, Y Jia, LG Hu, B Alva, A Fan, MS AF Gao, Yuan Jia, Liguo Hu, Bo Alva, Ashok Fan, Mingshou TI Potato Stolon and Tuber Growth Influenced by Nitrogen Form SO PLANT PRODUCTION SCIENCE LA English DT Article DE Ammonium; Nitrate; Potato; Shoot; Stolon; Tuber ID NET PHOTOSYNTHESIS; NITRATE; TUBERIZATION; NUTRITION; AMMONIUM; YIELD; TEMPERATURE; PHOTOPERIOD; INITIATION; RESPONSES AB Potato tuber initiation and its growth are key processes determining tuber yield, which are closely related to stolon growth, and are influenced by many factors including N nutrition. We investigated the influences of different forms of nitrogen (N) on stolon and tuber growth in sand culture with a nitrification inhibitor during 2010 - 2011, and using two potato cultivars. Plants supplied with NO3-N (N as nitrate, NO3-) produced more and thicker stolons than those supplied with NH4-N (N as ammonium, NH4+) at tuber initiation stage. In the plants fed NO3-N, the stolon tips swelled or formed tubers earlier and produced more tubers than in those fed with NH4-N. However, no significant difference was observed among N forms in terms of tuber yield at harvest, this may have been because of the shoot growth rate at tuber initiation stage was lower in the plants fed NO3-N. During the tuber bulking stage, the difference in shoot DWs among N forms began to decrease, and the shoot DW of plants fed NO3-N was even heavier than those fed NH4-N in some cases. The influence of N form on potato plant growth may therefore vary with the potato growth stage. C1 [Gao, Yuan; Jia, Liguo; Hu, Bo; Fan, Mingshou] Inner Mongolia Agr Univ, Coll Agr, Hohhot 010019, Inner Mongolia, Peoples R China. [Alva, Ashok] USDA ARS, Prosser, WA 99350 USA. RP Fan, MS (reprint author), Inner Mongolia Agr Univ, Coll Agr, Hohhot 010019, Inner Mongolia, Peoples R China. EM fmswh@126.com FU National Natural Science Foundation of China [31160411]; Pre-973 Project of China [2012CB126307]; Inner Mongolia Natural Science Foundation [2013ZD04] FX We are grateful to Prof. Yiwei Jiang from Purdue University, Indiana, USA, for his suggestions on this manuscript. This work has been supported by The National Natural Science Foundation of China (31160411), the Pre-973 Project of China (2012CB126307) and. Inner Mongolia Natural Science Foundation (2013ZD04). NR 25 TC 0 Z9 1 U1 1 U2 25 PU CROP SCIENCE SOC JAPAN PI TOKYO PA UNIV TOKYO-FACULTY AGRICULTURE BUNKYO-KU, TOKYO, 113, JAPAN SN 1343-943X J9 PLANT PROD SCI JI Plant. Prod. Sci. PD APR PY 2014 VL 17 IS 2 BP 138 EP 143 PG 6 WC Agronomy SC Agriculture GA AJ6EN UT WOS:000337782600004 ER PT J AU Brennan, EB Leap, JE AF Brennan, Eric B. Leap, Jim E. TI A Comparison of Drill and Broadcast Methods for Establishing Cover Crops on Beds SO HORTSCIENCE LA English DT Article DE organic farming; planting method; rye; seedling establishment; vegetable production; vetch ID PERENNIAL GRASS ESTABLISHMENT; WEED SUPPRESSION; SOWING METHOD; SEEDING RATE; PLANTING METHOD; SPRING WHEAT; SPATIAL UNIFORMITY; VARIETY AFFECTS; NITRATE LOSSES; WINTER-WHEAT AB Cover crop stands that are sufficiently dense soon after planting are more likely to suppress weeds, scavenge nutrients, and reduce erosion. Small-scale organic vegetable farmers often broadcast cover crop seed to establish cover crops but lack information on the most effective implements to incorporate the seed into the soil. Experiments were conducted with winter- and spring-sown cover crops to compare drilling vs. broadcasting methods for establishing rye (Secale cereale L.) mixed with either purple (Vicia benghalensis L., winter) or common vetch (V. sativa L., spring) on bed tops at a seeding rate of 140 kg.ha(-1) in Salinas, CA. Broadcast seed was incorporated with a rototiller, cultivator, or tandem disc. Cover crop stand uniformity was assessed visually, and cover crop emergence over time and seeding depth were measured. Stands were more uniform after drilling or broadcast + rototiller incorporation compared with the other methods. Cover crops emerged sooner and in higher densities after drilling compared with broadcasting. The delayed emergence of broadcast seed was most apparent during the cooler winter experiment, particularly with purple vetch. Most drilled seed emerged from 2-cm depth compared with the broadcast seed that emerged from up to 11-cm depth with the greatest variability after disc or rototiller incorporation. The data indicate that the cultivator and rototiller are preferable implements to incorporate broadcast seed on beds, but that 50% to 100% higher seeding rates for broadcasting than drilling are needed. The practical implications for weed and soil management, and planting costs are discussed. C1 [Brennan, Eric B.; Leap, Jim E.] ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. RP Brennan, EB (reprint author), ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM eric.brennan@ars.usda.gov NR 56 TC 1 Z9 1 U1 4 U2 12 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD APR PY 2014 VL 49 IS 4 BP 441 EP 447 PG 7 WC Horticulture SC Agriculture GA AI8VM UT WOS:000337205100006 ER PT J AU Sharma, K Merritt, JL Palmateer, A Goss, E Smith, M Schubert, T Johnson, RS van Bruggen, AHC AF Sharma, Kalpana Merritt, Joyce L. Palmateer, Aaron Goss, Erica Smith, Matthew Schubert, Tim Johnson, Robert S. van Bruggen, Ariena H. C. TI Isolation, Characterization, and Management of Colletotrichum spp. Causing Anthracnose on Lucky Bamboo (Dracaena sanderiana) SO HORTSCIENCE LA English DT Article DE Colletotrichum dracaenophilum; Dracaena marginata; emerging pathogens; fungicide; hot water treatment; red-edged dracaena ID ACUTATUM; SEED; TEMPERATURE; CALIFORNIA; PATHOGENS; NURSERIES; THAILAND; FOREST; DNA AB International trade in ornamental plants has increased worldwide. Dracaena is the genus most frequently imported into the United States and many pests and pathogens currently not in the United States could be imported with Dracaena plant materials. In 2009, lucky bamboo (Dracaena sanderiana) with anthracnose symptoms was found in Florida. The associated fungus, putative Colletotrichum dracaenophilum, is considered a reportable pathogen that originated from Asia. Imported D. sanderiana plants with anthracnose symptoms were collected from a nursery in south Florida in 2011 and retail stores in north Florida in 2012 and 2013. Five isolates of Colletotrichum spp. were isolated from symptomatic D. sanderiana plants and Koch's postulates were fulfilled for the first time. Polymerase chain reaction was performed on the internal transcribed space (ITS) and 28S rDNA regions of three original isolates and re-isolates and these sequences were compared with sequences of Colletotrichum spp. in GenBank. Sequence analysis indicated that the Colletotrichum isolates obtained from D. sanderiana in Florida belonged to C. dracaenophilum or the C. gloeosporioides species complex. Latent infections on healthy-looking lucky bamboo developed anthracnose lesions within 2 to 3 months. The reaction of several Dracaena species and varieties to the two Colletotrichum species was tested. Colletotrichum dracaenophilum caused the most severe disease on lucky bamboo, whereas one isolate of the C. gloeosporioides species complex was least pathogenic to all of the Dracaena spp. and varieties. Hot water treatments were not effective at controlling latent infections, but application of Azoxystrobin at 0.075 g a.i./L significantly reduced anthracnose development on both latently infected and inoculated lucky bamboo plants. C1 [Sharma, Kalpana; Goss, Erica; Smith, Matthew; van Bruggen, Ariena H. C.] Univ Florida, IFAS, Dept Plant Pathol, Gainesville, FL 32611 USA. [Merritt, Joyce L.] Santa Fe Coll, Dept Nat Sci, Gainesville, FL 32606 USA. [Palmateer, Aaron] Univ Florida, IFAS, Dept Plant Pathol, Trop Res & Educ Ctr, Homestead, FL 33031 USA. [Goss, Erica; van Bruggen, Ariena H. C.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32611 USA. [Schubert, Tim] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. [Johnson, Robert S.] US Anim & Plant Hlth Inspect Serv, USDA, Riverdale, MD 20737 USA. RP van Bruggen, AHC (reprint author), Univ Florida, IFAS, Dept Plant Pathol, 1453 Fifield Hall, Gainesville, FL 32611 USA. EM ahcvanbruggen@ufl.edu RI Smith, Matthew/A-8115-2012; Goss, Erica/H-7020-2014 OI Smith, Matthew/0000-0002-0878-0932; FU U.S. Department of Agriculture, Animal and Plant Health Inspection Service "Quality assessment of the U.S. Greenhouse Certification Program" FX This work was funded through a grant from the U.S. Department of Agriculture, Animal and Plant Health Inspection Service "Quality assessment of the U.S. Greenhouse Certification Program." NR 31 TC 1 Z9 1 U1 0 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD APR PY 2014 VL 49 IS 4 BP 453 EP 459 PG 7 WC Horticulture SC Agriculture GA AI8VM UT WOS:000337205100008 ER PT J AU Rinderer, TE De Guzman, LI Frake, AM Tarver, MR Khongphinitbunjong, K AF Rinderer, Thomas E. De Guzman, Lilia I. Frake, Amanda M. Tarver, Matthew R. Khongphinitbunjong, Kitiphong TI An Evaluation of the Associations of Parameters Related to the Fall of Varroa destructor (Acari: Varroidae) From Commercial Honey Bee (Hymenoptera: Apidae) Colonies as Tools for Selective Breeding for Mite Resistance SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Varroa destructor; Russian honey bee; mite fall; grooming behavior; Varroa resistance ID APIS-MELLIFERA COLONIES; JACOBSONI; INTERMISSA; TOLERANCE; BEHAVIOR; REMOVAL; TUNISIA; GROWTH; STOCKS; USA AB Varroa destructor (Anderson and Trueman) trapped on bottom boards were assessed as indirect measurements of colony mite population differences and potential indicators of mite resistance in commercial colonies of Russian and Italian honey bees (Apis mellifera L.) by using 35 candidate measurements. Measurements included numbers of damaged and nondamaged younger mites, nymphs, damaged and nondamaged older mites, fresh mites, and all mites, each as a proportion of total mites in the colonies and as a proportion of all trapped mites or all trapped fresh mites. Several measurements differed strongly between the stocks, suggesting that the detailed characteristics of trapped mites may reflect the operation of resistance mechanisms in the Russian honey bees. Regression analyses were used to determine the relationships of these candidate measurements with the number of mites in the colonies. The largest positive regressions differed for the two stocks (Italian honey bees: trapped mites and trapped younger mites; Russian honey bees: trapped younger mites and trapped fresh mites). Also, the regressions for Italian honey bees were substantially stronger. The largest negative regressions with colony mites for both stocks were for the proportion of older mites out of all trapped mites. Although these regressions were statistically significant and consistent with those previously reported, they were weaker than those previously reported. The numbers of mites in the colonies were low, especially in the Russian honey bee colonies, which may have negatively influenced the precision of the regressions. C1 [Rinderer, Thomas E.; De Guzman, Lilia I.; Frake, Amanda M.; Tarver, Matthew R.] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. [Khongphinitbunjong, Kitiphong] Chiang Mai Univ, Dept Biol, Fac Sci, Bee Protect Ctr, Chiang Mai 50200, Thailand. RP Rinderer, TE (reprint author), USDA ARS, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM tom.rinderer@ars.usda.gov NR 28 TC 3 Z9 3 U1 2 U2 23 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 516 EP 522 DI 10.1603/EC13477 PG 7 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700004 PM 24772529 ER PT J AU Rinderer, TE Danka, RG Johnson, S Bourgeois, AL Frake, AM Villa, JD De Guzman, LI Harris, JW AF Rinderer, Thomas E. Danka, Robert G. Johnson, Stephanie Bourgeois, A. Lelania Frake, Amanda M. Villa, Jose D. De Guzman, Lilia I. Harris, Jeffrey W. TI Functionality of Varroa-Resistant Honey Bees (Hymenoptera: Apidae) When Used for Western US Honey Production and Almond Pollination SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Varroa destructor; Apis mellifera; genetic resistance; honey production; almond pollination ID MULTISTATE FIELD TRIALS; WINTER COLONY LOSSES; INFORMED PARTNERSHIP; NATIONAL-SURVEY; NOSEMA-CERANAE; DESTRUCTOR; ARS; RESPONSES; STOCKS AB Two types of honey bees, Apis mellifera L., bred for resistance to Varroa destructor Anderson & Trueman, were evaluated for performance when used for honey production in Montana, and for almond pollination the following winter. Colonies of Russian honey bees and outcrossed honey bees with Varroa-sensitive hygiene (VSH) were compared with control colonies of Italian honey bees. All colonies were managed without miticide treatments. In total, 185 and 175 colonies were established for trials in 2010-2011 and 2011-2012, respectively. Survival of colonies with original queens or with supersedure queens was similaramongstocks for both years. Colony sizes of the Varroa-resistant stocks were as large as or larger than the control colonies during periods critical to honey production and almond pollination. Honey production varied among stocks. In the first year, all stocks produced similar amounts of honey. In the second year, Russian honey bees colonies produced less honey than the control colonies. V. destructor infestations also varied among stocks. In the first year, control colonies had more infesting mites than either of the Varroa-resistant stocks, especially later in the year. In the second year, the control and outcrossed Varroa-sensitive hygiene colonies had high and damaging levels of infestation while the Russian honey bees colonies maintained lower levels of infestation. Infestations of Acarapis woodi (Rennie) were generally infrequent and low. All the stocks had similarly high Nosema ceranae infections in the spring and following winter of both years. Overall, the two Varroa-resistant stocks functioned adequately in this model beekeeping system. C1 [Rinderer, Thomas E.; Danka, Robert G.; Johnson, Stephanie; Bourgeois, A. Lelania; Frake, Amanda M.; Villa, Jose D.; De Guzman, Lilia I.; Harris, Jeffrey W.] USDA ARS, Honey Bee Breeding Genet & Physiol Lab, Baton Rouge, LA 70820 USA. RP Rinderer, TE (reprint author), USDA ARS, Honey Bee Breeding Genet & Physiol Lab, 1157 Ben Hur Rd, Baton Rouge, LA 70820 USA. EM tom.rinderer@ars.usda.gov FU USDA-ARS Areawide Program "Improving Honey Bee Health, Survivorship and Pollination Availability" FX We thank Honey Land Apiaries (Jim and Craig Rodenberg) for providing the colonies used in these experiments and for valuable beekeeping collaboration. Jack Brumley kindly fed the colonies while they were wintering in California. Technical assistance was provided by U. S. Department of Agriculture-Agricultural Research Service (USDA-ARS) personnel, Lorraine Beaman, Garrett Dodds, David Dodge, Sandra Hineman, Victor Rainey, J. Anthony Stelzer, Joshua Wales, Jeremy Wagnitz, Rachel Watts, and Daniel Winfrey. Judy Chen (USDA-ARS, Beltsville, MD) measured virus titers. Funding was provided by the USDA-ARS Areawide Program "Improving Honey Bee Health, Survivorship and Pollination Availability." NR 29 TC 2 Z9 2 U1 5 U2 46 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 523 EP 530 DI 10.1603/EC13419 PG 8 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700005 PM 24772530 ER PT J AU Woods, JL Dreves, AJ James, DG Lee, JC Walsh, DB Gent, DH AF Woods, J. L. Dreves, A. J. James, D. G. Lee, J. C. Walsh, D. B. Gent, D. H. TI Development of Biological Control of Tetranychus urticae (Acari: Tetranychidae) and Phorodon humuli (Hemiptera: Aphididae) in Oregon Hop Yards SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE conservation biological control; cross-correlation; integrated pest management ID 2-SPOTTED SPIDER-MITE; PHYTOSEIID MITES; NATURAL ENEMIES; PREDATORS; COCCINELLIDAE; ORCHARDS; RELEASE; DENSITY AB The temporal development of biological control of arthropod pests in perennial cropping systems is largely unreported. In this study, the development of biological control of twospotted spider mite, Tetranychus urticae Koch, and hop aphid, Phorodon humuli (Schrank), in a new planting of hop in Oregon is described over a period of 9 yr (2005 2013). Both the abundance and diversity of natural enemies increased over time. Known predators of hop aphid (Coccinellidae and Antho-coridae) were present in all years; however, stable biological control of hop aphid was not achieved in most years and aphicides were required to suppress populations at commercially acceptable levels in 5 of 9 yr. Populations of aphidophagous coccinellids developed synchronously with hop aphid populations, and temporal correlations indicated these are the primary predatory insect associated with hop aphid regulation. However, sampling methods did not assess levels of aphid parasitoids and hyperparasitoids and their contribution to biological control was unquantified. Spider mite biological control was associated primarily with predatory mites (Phytoseiidae) and Stethorus spp. (Coccinellidae). The magnitude of temporal correlations of abundance of these predators with spider mites was found to be greatest on the same sampling dates and at lags of 7-14 d. Stable biological control of spider mites occurred after four field seasons, suppressing spider mites to levels similar to those commonly achieved with chemical control. A survey of 11 commercial hop yards in Oregon documented pest and natural enemy densities under commercial management practices over a period of 4 yr (2008-2011). Natural enemy abundance in commercial hop yards was similar to that of a 2- to 3-yr-old hop yard with limited disturbance. Whereas total reliance on biological control for hop aphid is unlikely to be successful, there appears to be unrealized potential for biological control of spider mites in commercial production. Dynamic action thresholds that consider the value of natural enemies are needed for both pests. C1 [Woods, J. L.; Dreves, A. J.] Oregon State Univ, Dept Crop & Soil Sci, Corvallis, OR 97331 USA. [James, D. G.; Walsh, D. B.] Washington State Univ, Dept Entomol, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. [Lee, J. C.] USDA ARS, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Gent, D. H.] Oregon State Univ, USDA ARS, Forage & Cereal Res Unit, Corvallis, OR 97331 USA. [Gent, D. H.] Oregon State Univ, USDA ARS, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Gent, DH (reprint author), Oregon State Univ, USDA ARS, Forage & Cereal Res Unit, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU Hop Research Council; Oregon State University; Washington Hop Commission; Washington State Commission on Pesticide Registration; Washington State University; U.S. Department of Agriculture Agricultural Research Service Current Research Information System [5358-21000-046-00D]; U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service Western IPM Center [2007-34103-18579] FX We gratefully acknowledge technical support that made this work possible. We also thank James Barbour and Megan Twomey for their review of an earlier draft of the manuscript. Funding for these studies was provided from the Hop Research Council, Oregon State University, Washington Hop Commission, Washington State Commission on Pesticide Registration, Washington State University, U.S. Department of Agriculture Agricultural Research Service Current Research Information System Project 5358-21000-046-00D, and U.S. Department of Agriculture Cooperative State Research, Education, and Extension Service Western IPM Center Award No. 2007-34103-18579. NR 45 TC 3 Z9 3 U1 5 U2 30 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 570 EP 581 PG 12 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700011 PM 24772536 ER PT J AU Behle, RW Jackson, MA AF Behle, Robert W. Jackson, Mark A. TI Effect of Fermentation Media on the Production, Efficacy, and Storage Stability of Metarhizium brunneum Microsclerotia Formulated as a Prototype Granule SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Metarhizium brunneum; fermentation; microsclerotia; Alphitobius diaperinus; lesser mealworm ID ALPHITOBIUS-DIAPERINUS PANZER; ANISOPLIAE METSCH. SOROK.; BASSIANA BALS. VUILL.; LESSER MEALWORM; BEAUVERIA-BASSIANA; HYPOCREALES CLAVICIPITACEAE; ENTOMOPATHOGENIC FUNGI; POULTRY LITTER; SUGAR-BEET; COLEOPTERA AB New liquid fermentation techniques for the production of the bioinsecticidal fungus Metarhizium brunneum strain F-52 have resulted in the formation of microsclerotia (MS), a compact, melonized-hyphal structure capable of surviving desiccation and formulation as dry granules. When rehydrated, these MS granules germinate to produce conidia that can infect susceptible insects. Fermentation media containing cottonseed or soy flours as nitrogen sources and formulated at two carbon to nitrogen ratios(C:N), 30:1 or 50:1, were evaluated for production of microsclerotia. Dry MS granule samples were compared for storage stability based on conidia production, and insecticidal activity against larvae of the lesser mealworm, Alphitobius diaperinus (Panzer), using a potting soil bioassay. Cottonseed and soy flours were equivalent for production, MS granule viability, and insecticidal activity. Fermentation media containing higher nitrogen concentrations (30:1 C:N) resulted in greater biomass accumulation and greater production of conidiafromgranules regardless of the nitrogen source. MS granules made with M. brunneum cultures grown in media with 30:1 C:N produced 8.5 x 10(9) conidia per gram of granules after 8-d incubation, significantly higher than MS granules made using fungus produced using 50:1 C:N media (5.5 x 10(9) conidia per gram dry MS granules). The LC50 for larval mortality was 8.05 x 10(5) conidia per cup, equivalent to applications of 94 or 147 mu g granules per cup for granules made from high and low nitrogen media, respectively. Measurements of water activity were not significantly different among granule samples (0.28-0.29) even though granules made from high nitrogen media had higher moisture content (> 5.2%) compared with granules made from low nitrogen media (< 4.6%). Higher initial conidial production was reflected in longer storage stability at 25 degrees C, with half-lives estimated at 3.7 and 1.7 wk for 30:1 and 50:1 C:N ratios, respectively. These results support further evaluation of MS granule formulations for the control of soil-inhabiting insect pests. C1 [Behle, Robert W.; Jackson, Mark A.] USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, Peoria, IL 61604 USA. RP Behle, RW (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Crop Bioprotect Res Unit, 1815 North Univ Ave, Peoria, IL 61604 USA. EM robert.behle@ars.usda.gov NR 36 TC 3 Z9 4 U1 5 U2 22 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 582 EP 590 DI 10.1603/EC13426 PG 9 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700012 PM 24772537 ER PT J AU Mangan, RL Thomas, DB AF Mangan, Robert L. Thomas, Donald B. TI Comparison of Torula Yeast and Various Grape Juice Products as Attractants for Mexican Fruit Fly (Diptera: Tephritidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE bait; Valencia orange; Mexican fruit fly; Nuevo Leon; San Luis Potosi ID CERATITIS-CAPITATA; ATTRACTIVENESS; TRAPS; LURES; HOST AB Early research investigating attractants for the Mexican fruit fly, Anastrepha ludens Loew, during the 1930s indicated that fermentation products were effective attractants for Mexican fruit flies and other tropical Tephritidae, but that attraction to fruit components was only of academic interest. Tests reported here were carried out on populations of Mexican fruit flies from 2004 to 2011. Trapping experiments carried out at sites in the states Nuevo Leon and San Luis Potosi compared grape juice, reconstituted grape concentrate and powdered grape mixes, and torula yeast extract in orchards at each site. The Nuevo Leon orchard was mixed with alternate rows of pears and surrounded by alternate hosts. The San Luis Potosi site was surrounded by other orange orchards or nonhosts. Each test was run for at least 10 mo and included highest and lowest trapping periods. Results showed that grape juice captured the most total flies and had the fewest samples with zero flies. However, in the series of experiments, each product had the most captures in at least one experiment. Hydrolyzed torula was superior in one of the six experiments. In five of the tests, polyethylene glycol was tested as an additive to the grape products but never improved capture rate compared with the product without the additive. These results indicate that grape juice is superior to grape concentrate or powder and grape juice is at least equal to torula yeast hydrolysate for trapping pest populations of Mexican fruit flies in commercial citrus orchards. C1 [Mangan, Robert L.; Thomas, Donald B.] Kika de la Garza Subtrop Agr Res Ctr, Crop Qual & Fruit Insects Res Unit, Weslaco, TX 78501 USA. RP Mangan, RL (reprint author), USDA ARS, Subtrop Hort Res Stn, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM robert.mangan@ars.usda.gov NR 30 TC 2 Z9 2 U1 2 U2 9 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 591 EP 600 DI 10.1603/EC12376 PG 10 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700013 PM 24772538 ER PT J AU Landolt, P Cha, D Davis, TS AF Landolt, Peter Cha, Dong Davis, Thomas S. TI Attraction of the Orange Mint Moth and False Celery Leaftier Moth (Lepidoptera: Crambidae) to Floral Chemical Lures SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE orange mint moth; false celery leaftier; attractant; kairomone; trap ID CABBAGE-LOOPER MOTHS; FEEDING ATTRACTANTS; ALFALFA LOOPER; BINARY BLENDS; NOCTUIDAE; PHENYLACETALDEHYDE; FLOWERS; IDENTIFICATION; PYRALIDAE; ODORANTS AB Orange mint moths, Pyrausta orphisalis (Walker) (Crambidae), were initially trapped in a study of noctuid moth attraction to floral volatiles. A subsequent series of trapping experiments in commercial mint fields determined that phenylacetaldehyde and 4-oxoisophorone were attractive to P. orphisalis, whereas benzyl acetate, eugenol, cis-jasmone, limonene, linalool, methyl-2-methoxybenzoate, methyl salicylate, beta-myrcene, and 2-phenylethanol were not. When used in combination with phenylacetaldehyde, 4-oxoisophorone and methyl-2-methoxybenzoate increased catches of P. orphisalis in traps by similar to 50%, and beta-myrcene tripled the trap catch. A second crambid species, the false celery leaftier moth, Udea profundalis Packard, was also attracted to phenylacetaldehyde, but was not attracted to any other single-chemical lure. Cis-jasmone, limonene, and 4-oxoisophorone increased catches of U. profundalis by similar to 50% when presented in traps with phenylacetaldehyde, while linalool increased the catch 2.5-fold, and beta-myrcene tripled the trap catch. Both sexes of each species were similarly attracted to most of these lures. These findings provide chemical lures for trapping males and females of both P. orphisalis and U. profundalis. C1 [Landolt, Peter; Cha, Dong] USDA ARS, Wapato, WA 98951 USA. RP Landolt, P (reprint author), USDA ARS, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM peter.landolt@ars.usda.gov NR 30 TC 0 Z9 1 U1 0 U2 10 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 654 EP 660 DI 10.1603/EC13535 PG 7 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700021 PM 24772546 ER PT J AU Crook, DJ Hull-Sanders, HM Hibbard, EL Mastro, VC AF Crook, Damon J. Hull-Sanders, Helen M. Hibbard, Emily L. Mastro, Victor C. TI A Comparison of Electrophysiologically Determined Spectral Responses in Six Subspecies of Lymantria SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE electroretinogram; spectral sensitivity; gypsy moth; Lymantria ID COLOR-VISION; COMPOUND EYE; GYPSY MOTHS; LEPIDOPTERA; SENSITIVITY; DISPAR; ILLUMINATION; FLIGHT; COSTS AB The spectral sensitivity of the compound eye in three gypsy moth species from six different geographical regions (Lymantria dispar asiatica Vnukovskij [Asian gypsy moth], Lymantria dispar japonica Motschulsky [Japanese gypsy moth], and Lymantria dispar dispar L. [North American gypsy moth]) was tested electrophysiologically in the wavelength region 300D700 nm. For all moths examined, a maximum response occurred in the 480D520-nm range (blue-green region) with a shoulder peak occurring at 460 nm. A smaller, secondary peak was observed for both sexes at the 340D380-nm range, which is in the region considered behaviorally maximal in night-flying insects. No peaks in sensitivity were observed between 520 and 700 nm (red region) for any of the moths tested. Based on our retinal recording data, a short wavelength blocking filter with a transition wavelength near 500 nm should reduce gypsy moth attraction to artificial lighting sources. This would help reduce the number of Lymantria-infested ships traveling to and from foreign ports. C1 [Crook, Damon J.; Hull-Sanders, Helen M.; Hibbard, Emily L.; Mastro, Victor C.] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. RP Crook, DJ (reprint author), USDA APHIS PPQ CPHST, Otis Lab, Bldg 1398, Buzzards Bay, MA 02542 USA. EM damon.j.crook@aphis.usda.gov FU U.S. Department of Agriculture-The Animal and Plant Health Inspection Service-Plant Protection and Quarantine-The Center for Plant Health Science and Technology research program FX This research was funded by the U.S. Department of Agriculture-The Animal and Plant Health Inspection Service-Plant Protection and Quarantine-The Center for Plant Health Science and Technology research program. Wethank Joe Francese for reviewing an earlier draft of the manuscript. We also thank the two anonymous reviewers for comments on the manuscript. NR 35 TC 0 Z9 0 U1 3 U2 13 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 667 EP 674 DI 10.1603/EC13464 PG 8 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700023 PM 24772548 ER PT J AU Stilwell, AR Smith, SM Cognato, AI Martinez, M Flowers, RW AF Stilwell, Abby R. Smith, Sarah M. Cognato, Anthony I. Martinez, Malena Flowers, R. Wills TI Coptoborus ochromactonus, n.sp (Coleoptera: Curculionidae: Scolytinae), an Emerging Pest of Cultivated Balsa (Malvales: Malvaceae) in Ecuador SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE ambrosia beetle; scolytidae; plant disease; wilt; xyleborini ID AMBROSIA; FUNGI AB A new species of xyleborine ambrosia beetle has been found to attack balsa, Ochroma pyramidale (Cavanilles ex Lamarck) Urban, in Ecuador. Coptoborus ochromactonus Smith & Cognato is described and its biology is reported. Large-scale surveys were conducted between 2006 and 2009, and observational studies were carried out between 2010 and 2013 in Ecuadorian commercial plantations to determine life history and host preference characteristics. C. ochromactonus attacked balsa between 1.5 and 3 yr in age. Successful attacks were more prevalent in smaller diameter trees and unhealthy trees. In general, attacks and beetle-caused mortality were more prevalent during the dry summer months when trees were under more moisture and light stress. Fungal mycelia were consistently observed coating beetle galleries and are likely the true damaging agent to balsa trees. C1 [Stilwell, Abby R.] 3A Composites Res & Dev, Guayaquil, Ecuador. [Smith, Sarah M.; Cognato, Anthony I.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Martinez, Malena] Univ Tecn Estatal Quevedo, Quevedo, Los Rios, Ecuador. [Flowers, R. Wills] Univ Tecn Estatal Quevedo, Proyecto PROMETEO Secretaria Educ Super Ciencias, Quevedo, Los Rios, Ecuador. RP Stilwell, AR (reprint author), USDA ARS, Carl Hayden Bee Res Ctr, 2000 East Allen Dr, Tucson, AZ 85719 USA. EM abbyrstilwell@gmail.com FU Proyecto Prometeo of the Secretaria de Educacion Superior, Ciencia y Tecnologia, Quito; United States Department of Agriculture-Forest Service Early Detection Rapid Response (USDAD FS EDRR) program [11-DG-11420004-257] FX We thank Plantabal S.A. (3A Composites) of Ecuador for providing 2006-2009 survey data used in this article. Special thanks go to Jorge Venegas and Sergio Cedeno of Plantabal S. A. for providing information relevant to this article. We also thank Xavier Chiriboga of Agencia Ecuatoriana de Aseguramiento de la Calidad del Agro (AGROCALIDAD) for submitting specimens. Natalia Vandenberg provided access to specimens at the USNM. This research was financed in part by the Proyecto Prometeo of the Secretaria de Educacion Superior, Ciencia y Tecnologia, Quito, Ecuador and by the United States Department of Agriculture-Forest Service Early Detection Rapid Response (USDAD FS EDRR) program (11-DG-11420004-257). NR 32 TC 0 Z9 0 U1 1 U2 4 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 675 EP 683 DI 10.1603/EC13559 PG 9 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700024 PM 24772549 ER PT J AU Lapointe, SL Stelinski, LL Keathley, CP Mafra-Neto, A AF Lapointe, S. L. Stelinski, L. L. Keathley, C. P. Mafra-Neto, A. TI Intentional Coverage Gaps Reduce Cost of Mating Disruption for Phyllocnistis citrella (Lepidoptera: Gracillariidae) in Citrus SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE sex pheromone; (Z,Z,E)-7,11,13-hexadecatrienal; citrus leafminer; SPLAT; Lepidoptera ID SEX-PHEROMONE; CODLING MOTH; PEAR ESTER; LEAFMINER; DISPENSERS; ORCHARDS; IDENTIFICATION; TORTRICIDAE; CANKER AB The leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae), is a global pest of citrus and contributes to the incidence and severity of citrus bacterial canker. SPLAT CLM (ISCA Technologies, Riverside, CA) is an emulsified wax that provides sustained release of (Z,Z,E)-7,11,13-hexadecatrienal, the major component of P. citrella sex pheromone. Trials in commercial orchards demonstrated that SPLAT CLM applied to plots of varying width resulted in disruption of trap catch of male P. citrella within treated rows and across untreated rows adjacent to treated rows. SPLAT CLM applied to plots of constant width (10 rows) disrupted trap catch across an untreated gap as the square of the width of the gap. Similarly, the ability of the pheromone source in treated rows to disrupt trap catch across untreated gaps of constant size declined as the square of the width of adjacent treated areas. A coverage pattern of 4 rows skipped for every 10 treated rows resulted in a 4% reduction of trap shutdown, and reduced the product and application costs by 29%. Mining incidence by P. citrella in treated rows was reduced by 53% compared with untreated areas. Intentional coverage gaps can significantly reduce the cost of mating disruption. Commercial lures for P. citrella used in this study were highly potent with respect to attracting males. Each lure was approximate to 10(3) times as attractive as an individual P. citrella female. Disruption of trap catch using commercial lures may underestimate actual mating disruption achieved in the field. C1 [Lapointe, S. L.] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Stelinski, L. L.] Univ Florida, Dept Entomol & Nematol, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Keathley, C. P.] USDA ARS, Ft Pierce, FL 34945 USA. [Mafra-Neto, A.] ISCA Technol Inc, Riverside, CA 92507 USA. RP Lapointe, SL (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM stephen.lapointe@ars.usda.gov FU Specialty Crops Block Grant; USDA; Florida Department of Agriculture and Consumer Services FX Larry Markle, Julia Morris, Matt Chellemi, Paul Robbins and Rocco Alessandro (USDADARS, Ft. Pierce, FL), Wendy Meyer, Siddharth Tiwari, and Ian Jackson (University of Florida, Lake Alfred, FL) provided technical assistance in the field. Dave Robinson (International Fly Masters, Ft. Pierce, FL) provided prototype machinery and made the field applications. Significant funding was provided by the Specialty Crops Block Grant, USDA and the Florida Department of Agriculture and Consumer Services. We are particularly grateful to Tom Stopyra (The Packers of Indian River, Fort Pierce, FL) and David Kemeny (TRB Groves, Punta Gorda, FL) for arranging access to those properties. Mention of a trademark or proprietary product does not constitute a guarantee or warranty of the product by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 19 TC 4 Z9 4 U1 1 U2 10 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 718 EP 726 DI 10.1603/EC13388 PG 9 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700029 PM 24772554 ER PT J AU Osbrink, WLA Cornelius, ML Showler, AT Pound, JM AF Osbrink, W. L. A. Cornelius, M. L. Showler, A. T. Pound, J. M. TI Effects of a Fipronil Spot Treatment on Field Colonies of Coptotermes formosanus (Isoptera: Rhinotermitidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Formosan subterranean termite; colony effect; nonrepellent ID SUBTERRANEAN TERMITES ISOPTERA; RETICULITERMES-VIRGINICUS ISOPTERA; NONREPELLENT TERMITICIDES; SHIRAKI ISOPTERA; NEW-ORLEANS; INDEPENDENT MONITORS; FLAVIPES ISOPTERA; ALATE PRODUCTION; LOUISIANA; EASTERN AB This field study investigated the colony effect of a fipronil spot treatment applied to active infestations of Formosan subterranean termite, Coptotermes formosanus Shiraki. Spot treatments were applied to a single active independent monitor from each of four colonies in which multiple independent monitors were established. All treated monitors were abandoned, and the contents of the treated monitors were replaced with untreated wood at the approximate to 30-d posttreatment inspection. All colonies survived treatment and only one colony exhibited long- term effects, which included significant reductions in termite collections and increased worker size. The affected colony was treated within 1 m of its primary nest. Two colonies exhibited a correlation between monitor termite production and distance from treatment. Distance appears to be a factor limiting fipronil's colony effects. The Formosan subterranean termite may not be a good candidate for the exterior perimeter and localized interior treatment label option because of the large range and size of the colony. C1 [Osbrink, W. L. A.; Showler, A. T.; Pound, J. M.] USDA ARS SPA Knipling Bushland US Livestock Insec, Kerrville, TX 78028 USA. [Cornelius, M. L.] USDA ARS BARC, Beltsville, MD 20705 USA. RP Osbrink, WLA (reprint author), USDA ARS SPA Knipling Bushland US Livestock Insec, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM weste.osbrink@ars.usda.gov NR 60 TC 2 Z9 2 U1 0 U2 5 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 727 EP 740 PG 14 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700030 PM 24772555 ER PT J AU Petzold-Maxwell, JL Siegfried, BD Hellmich, RL Abel, CA Coates, BS Spencer, TA Gassmann, AJ AF Petzold-Maxwell, Jennifer L. Siegfried, Blair D. Hellmich, Richard L. Abel, Craig A. Coates, Brad S. Spencer, Terrence A. Gassmann, Aaron J. TI Effect of Maize Lines on Larval Fitness Costs of Cry1F Resistance in the European Corn Borer (Lepidoptera: Crambidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Bacillus thuringiensis; Cry1F; ecological negative cross-resistance; Ostrinia nubilalis; resistance management ID OSTRINIA-NUBILALIS LEPIDOPTERA; DIAMONDBACK MOTH LEPIDOPTERA; BACILLUS-THURINGIENSIS TOXIN; FIELD-DERIVED STRAIN; INSECT RESISTANCE; COLEOPTERA-CHRYSOMELIDAE; ROOTWORM COLEOPTERA; CROSS-RESISTANCE; TRANSGENIC MAIZE; PINK-BOLLWORM AB Crops producing insecticidal toxins from the bacterium Bacillus thuringiensis (Bt) are widely planted and enable management of key insect pests while reducing the use of conventional insecticides. However, the evolution of Bt resistance could diminish these benefits. Fitness costs of Bt resistance occur in the absence of Bt toxin when individuals with resistance alleles show a reduction in fitness relative to susceptible individuals, and they can delay the evolution of resistance. Ecological factors including host-plant variety can affect the magnitude of fitness costs, and consequently, the degree to which fitness costs delay resistance. In this study, we measured fitness costs of resistance to Bt toxin Cry1F in the European corn borer Ostrinia nubilalis Hubner (Lepidoptera: Crambidae) using Cry1F-resistant and Cry1F-susceptible strains sharing a similar genetic background. Fitness costs were tested on three lines of maize, Zea mays L., by measuring larval survival and development in two greenhouse experiments with plants in either the vegetative or reproductive stage. Both experiments showed that maize line significantly affected larval survival and developmental rate. However, larval survival, mass, and developmental rate did not differ between the Cry1F-resistant and susceptible strains, indicating a lack of fitness costs of resistance to Cry1F for the larval fitness components measured in this experiment. Future experiments should test for fitness costs of Cry1F resistance affecting survival to adulthood and adult life-history parameters. C1 [Petzold-Maxwell, Jennifer L.; Hellmich, Richard L.; Abel, Craig A.; Coates, Brad S.; Gassmann, Aaron J.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Siegfried, Blair D.; Spencer, Terrence A.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [Hellmich, Richard L.; Abel, Craig A.; Coates, Brad S.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. RP Petzold-Maxwell, JL (reprint author), Wartburg Coll, Dept Biol, 100 Wartburg Blvd, Waverly, IA 50677 USA. EM jennifer.maxwell@wartburg.edu FU U. S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA) [2010-33522-21673]; USDA, dAgricultural Research Service (CRIS) [3625-22000-017-00D] FX We thank Keith Bidne, Sean Bradley, Landon Momberg, and Hannah Owens for their assistance. This research was supported by Biotechnology Risk Assessment Grant Program competitive grant 2010-33522-21673 from the U. S. Department of Agriculture-National Institute of Food and Agriculture (USDA-NIFA). We gratefully acknowledge Dow Agro-Sciences for providing access to the resistant strain of O. nubilalis. Contributions were also made from the University of Nebraska, Department of Entomology, and the USDA, Agricultural Research Service (CRIS Project 3625-22000-017-00D). NR 52 TC 5 Z9 5 U1 0 U2 22 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 764 EP 772 DI 10.1603/EC13359 PG 9 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700034 PM 24772559 ER PT J AU Prabhaker, N Castle, S Perring, TM AF Prabhaker, Nilima Castle, Steven Perring, Thomas M. TI Baseline Susceptibility of Bemisia tabaci B Biotype (Hemiptera: Aleyrodidae) Populations From California and Arizona to Spirotetramat SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE whitely immature; Movento; monitoring; systemic insecticide; resistance ID INSECT GROWTH-REGULATORS; MANAGING RESISTANCE; HOMOPTERA; PYRIPROXYFEN; CICADELLIDAE; SPIROMESIFEN; MANAGEMENT; BUPROFEZIN; TOXICITY; IMMATURE AB Baseline toxicity levels to foliarly applied spirotetramat were established for 19 field populations of whiteflies, Bemisia tabaci (Gennadius) B biotype (= Bemisia argentifolii Bellows & Perring) (Hemiptera: Aleyrodidae) from Arizona and California in 2008 and 2009. The susceptibility data were determined against the second instar of B. tabaci field collections before the registration and widespread use of spirotetramat in California. Three strains of whitefly, resistant to either bifenthrin, imidacloprid, or pyriproxyfen, were also tested to determine the potential for cross-resistance to spirotetramat. No significant geographic variation in susceptibility to spirotetramat was observed among regions within Arizona. The LC50 values for the Arizona populations spanned a 14-fold range between populations during the 2 yr sampling tests including a low LC50 of 0.91 (mu g [AI] ml(-1)) and a high LC50 of 13.47 (mu g [AI] ml(-1)), while the LC 90 values showed a seven-fold range. The field populations from California exhibited limited variation in susceptibility to spirotetramat in general (1.02-7.02 mu g [AI] ml(-1)) with one exception (27.98 mu g [AI] ml(-1)). Variation in susceptibility among the resistant strains was about eight-fold at the LC 50 level with the PYR-strain, showing the highest susceptibility to spirotetramat at 3.79 (mu g [AI] ml(-1)). In addition, comparisons of relative susceptibilities among three older immature instars of two field populations showed no significant differences. These results establish a regional baseline that can serve as a reference for future monitoring and management of B. tabaci resistance to spirotetramat. C1 [Prabhaker, Nilima; Perring, Thomas M.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Castle, Steven] USDA ARS, ALARC, Maricopa, AZ 85239 USA. RP Prabhaker, N (reprint author), Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. EM nilima.prabhaker@ucr.edu NR 40 TC 2 Z9 5 U1 1 U2 19 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 773 EP 780 PG 8 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700035 PM 24772560 ER PT J AU Elliott, NC Brewer, MJ Giles, KL Backoulou, GF McCornack, BP Pendleton, BB Royer, TA AF Elliott, N. C. Brewer, M. J. Giles, K. L. Backoulou, G. F. McCornack, B. P. Pendleton, B. B. Royer, T. A. TI Sequential Sampling for Panicle Caterpillars (Lepidoptera: Noctuidae) in Sorghum SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE integrated pest management (IPM); sequential sampling; corn earworm; fall armyworm; Helicoverpa zea ID CORN-EARWORM; HELICOVERPA-ZEA; GRAIN SORGHUM; INSECT PESTS; MODELS AB Panicle caterpillars comprise an economically important insect pest complex of sorghum throughout the Great Plains of the United States, particularly in Kansas, Oklahoma, and Texas. The sorghum panicle caterpillar complex consists of larvae of two polyphagous lepidopteran species: the corn earworm, Helicoverpa zea (Boddie), and fall armyworm, Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae). Sampling for panicle caterpillars in sorghum fields is usually accomplished by the beat bucket sampling technique with a fixed sample size of 30 beat bucket samples of one sorghum panicle each per 16.2 ha of field. We used WaldOs sequential probability ratio test for a negative binomial distribution to develop a sequential sampling plan for panicle caterpillars. In total, 115 sorghum fields were sampled in Kansas, Oklahoma, and Texas from June to August 2010. Panicle caterpillars had an aggregated distribution of counts confirmed by PearsonOs chi-square statistic for lack of fit to the negative binomial distribution for each sampled field. A sequential sampling plan was developed using a high threshold (an economic threshold) of 0.5 caterpillars per sorghum panicle, a low threshold (a safe level) of 0.20 caterpillars per panicle, and fixed error rates (alpha = 0.10 and beta = 0.05). At caterpillar densities > 0.45 and < 0.12 per panicle, the average number of panicles inspected to make a decision was less than the current recommendation of 30. In a 2013 validation test of 25 fields, the expected number of samples taken from average sample number curve was in close agreement with the number of samples required using the sequential plan (r(2) = 0.93), and all fields were correctly classified when compared with a fixed sample size result. The plan improved upon current sampling recommendations for panicle caterpillars in sorghum because at known acceptable fixed error rates fewer samples were required when caterpillars are scarce or abundant, whereas more samples were required to make decisions with the same acceptable error rates when densities were near the economic thresholds. C1 [Elliott, N. C.] USDA ARS, Plant Sci Res Lab, Stillwater, OK 74075 USA. [Brewer, M. J.] Texas AgriLife Res & Extension Ctr Corpus Christi, Corpus Christi, TX 78406 USA. [Giles, K. L.; Backoulou, G. F.; Royer, T. A.] Oklahoma State Univ, Dept Entomol & Plant Pathol, Stillwater, OK 74078 USA. [McCornack, B. P.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Pendleton, B. B.] West Texas A&M Univ, Dept Agr Sci, Canyon, TX 79016 USA. RP Elliott, NC (reprint author), USDA ARS, Plant Sci Res Lab, 1301 N Western Rd, Stillwater, OK 74075 USA. EM norman.elliott@ars.usda.gov RI Royer, Tom/D-9737-2016 OI Royer, Tom/0000-0003-0912-7115 FU United Sorghum Checkoff Program [R0008-10] FX We thank Tim Johnson, Darwin Anderson, James Glover, Luke Pruter, Kent Hampton, Alysha Soper, Melody Larsen, and Jody Gilchrest for assistance with data collection. This research was funded by grant R0008-10 from the United Sorghum Checkoff Program. Contribution no. 14-095-J from the Kansas Agricultural Experiment Station. NR 25 TC 1 Z9 1 U1 2 U2 11 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 846 EP 853 DI 10.1603/EC13413 PG 8 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700044 PM 24772569 ER PT J AU Athanassiou, CG Kavallieratos, NG Arthur, FH Throne, JE AF Athanassiou, Christos G. Kavallieratos, Nickolas G. Arthur, Frank H. Throne, James E. TI Residual Efficacy of Chlorfenapyr for Control of Stored-Product Psocids (Psocoptera) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE chlorfenapyr; Liposcelis; surface treatment; Psocoptera ID LIPOSCELIS-ENTOMOPHILA; STRUCTURAL TREATMENTS; TRIBOLIUM-CASTANEUM; SPECIES PSOCOPTERA; GRAIN PROTECTANTS; POPULATION-GROWTH; STEEL BINS; CONCRETE; INSECTICIDES; WHEAT AB The residual effect of chlorfenapyr (Phantom) was evaluated for residual control of three stored-product psocid species: Liposcelis bostrychophila Badonnel, Liposcelis entomophila (Enderlein), and Liposcelis paeta Pearman (Psocoptera: Liposcelididae). Chlorfenapyr was applied to individual arenas with a concrete surface at rates of 0, 2.8, 13.8, 20.6, 27.5, 55, and 110 mg active ingredient (AI)/m(2). Adults were exposed on the treated arenas and mortality assessed after 1, 2, and 3 d. The procedures were repeated weekly on the same treated arenas for 3 wk to assess residual efficacy. At each week, mortality of all species was low after 1 d of exposure but notably increased after 2 or 3 d. L. entomophila was the most susceptible species, with 99 100% mortality at rates of 13.8 mg/m(2) or higher. Similarly, mortality of L. paeta after 3 dof exposure at the same concentration ranged from 92 to 100%. L. bostrychophila was the least susceptible species, with mortality of <60% during the third week after application at rates <= 27.5 mg/m(2). However, even for this species, mortality after 3 wk was 90% or higher at rates > 27.5 mg/m(2). Complete mortality of all species occurred after 3 d exposure at the highest rate tested of 110 mg/m(2). Thus, our results show that chlorfenapyr is effective against major psocid species at the application rates evaluated in this study. C1 [Athanassiou, Christos G.] Univ Thessaly, Dept Agr Crop Prod & Rural Dev, Lab Entomol & Agr Zool, Nea Ionia Magnissia 38446, Greece. [Athanassiou, Christos G.; Kavallieratos, Nickolas G.; Arthur, Frank H.; Throne, James E.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Kavallieratos, Nickolas G.] Benaki Phytopathol Inst, Dept Entomol & Agr Zool, Lab Agr Entomol, Kifisia 14561, Attica, Greece. [Throne, James E.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Athanassiou, CG (reprint author), Univ Thessaly, Dept Agr Crop Prod & Rural Dev, Lab Entomol & Agr Zool, Phytokou Str, Nea Ionia Magnissia 38446, Greece. EM athanassiou@agr.uth.gr FU United States Department of Agriculture - Agricultural Research Service [G-3-10895] FX We thank Ann Redmon and Kris Hartzer for technical assistance. The first author thanks United States Department of Agriculture - Agricultural Research Service for providing the financial support that made this work possible, through the G-3-10895 program. NR 24 TC 5 Z9 5 U1 0 U2 6 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 854 EP 859 DI 10.1603/EC13376 PG 6 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700045 PM 24772570 ER PT J AU Diaz-Montano, J Campbell, JF Phillips, TW Throne, JE AF Diaz-Montano, John Campbell, James F. Phillips, Thomas W. Throne, James E. TI Evaluation of Potential Attractants for Liposcelis bostrychophila (Psocoptera: Liposcelididae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE booklice; monitoring; attractant; grain; stored product ID STORED-PRODUCT PSOCIDS; BADONNEL PSOCOPTERA; CONTROLLED-ATMOSPHERE; POPULATION-GROWTH; ESSENTIAL OILS; COMPARATIVE REPELLENCY; SUBSTRATE SELECTION; SPECIES PSOCOPTERA; BOOK-PAPER; WHEAT AB The psocid, Liposcelis bostrychophila Badonnel (Psocoptera: Liposcelididae), can cause significant damage to stored commodities, and its pest status in the United States has been increasing over the last decade. Because L. bostrychophila is difficult to control with conventional methods, it is critical to explore alternative approaches such as the use of attractants that can be incorporated into integrated pest management programs for monitoring psocids. The orientation response of several L. bostrychophila life stages (first and second instars, third and fourth instars, 0- to 7-d-old adults, 21-to 28-d-old adults, and adults of mixed ages) to a range of potential attractants (including whole and cracked grains, grain-based oils, wheat germ, brewer's yeast, and commercially available kairomone lures) was studied using a two-choice pitfall test to identify candidates for further development as lures in traps. Among the potential attractants evaluated, the strongest response by all stages of L. bostrychophila was to brewer's yeast. Other materials for which there was consistently a strong response were psocid diet, wheat germ, and wheat germ oil. These results show the potential for developing monitoring tools for integrated pest management programs for L. bostrychophila and other psocid species. C1 [Diaz-Montano, John; Campbell, James F.; Throne, James E.] USDA ARS, Stored Prod Insect Res Unit, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Phillips, Thomas W.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. RP Diaz-Montano, J (reprint author), USDA ARS, Stored Prod Insect Res Unit, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM john.diaz-montano@ars.usda.gov NR 52 TC 9 Z9 9 U1 0 U2 10 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 867 EP 874 DI 10.1603/EC13427 PG 8 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700047 PM 24772572 ER PT J AU Woods, JL Gent, DH AF Woods, J. L. Gent, D. H. TI Suppression of Hop Looper (Lepidoptera: Noctuidae) by the Fungicide Pyraclostrobin SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE integrated pest management; Humulus lupulus; nontarget impact ID HYPENA-HUMULI; ACARI TETRANYCHIDAE; NATURAL ENEMIES; PHENOLOGY; USA AB The hop looper, Hypena humuli Harris, is a reemergent pest of hop that often requires treatment to mitigate crop damage. In 4 yr of field trials, plots treated with fungicides were observed to sustain less hop looper defoliation compared with nontreated plots. Further investigation revealed that abundance of hop looper and associated defoliation were reduced when the fungicide pyraclostrobin was applied in late July to early August. Two other fungicides possessing active ingredients in the same chemical family (quinone outside inhibitor) did not reduce abundance of hop looper or its defoliation. Pyraclostrobin is efficacious against powdery mildew diseases, and the application timing evaluated in these studies corresponds with a period of juvenile susceptibility of hop cones to the disease. Use of fungicides containing pyraclostrobin at this time may have the ancillary benefit of reducing hop looper damage, potentially obviating the need for broad- spectrum insecticides later in the season. Follow-up studies are warranted to determine whether pyraclostrobin may inhibit other lepidopteran species. C1 [Woods, J. L.; Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gent, D. H.] USDA ARS, Forage & Cereal Res Unit, Corvallis, OR 97331 USA. RP Gent, DH (reprint author), USDA ARS, Forage & Cereal Res Unit, Corvallis, OR 97331 USA. EM gentd@onid.orst.edu FU U. S. Department of Agriculture-Agricultural Research Service-Current Research Information System (USDA-ARS-CRIS) [5358-21000-046-00D] FX The authors gratefully acknowledge technical support from Nanci Adair, Kariza King, Suzy Kropf, Kazandra Lewis, Genevieve Martinusen, and Megan Twomey. We also thank Tessa Grasswitz and David James for their review of an earlier draft of the manuscript. Support for these studies was provided by U. S. Department of Agriculture-Agricultural Research Service-Current Research Information System (USDA-ARS-CRIS) Project 5358-21000-046-00D. NR 22 TC 0 Z9 0 U1 2 U2 10 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD APR PY 2014 VL 107 IS 2 BP 875 EP 879 DI 10.1603/EC13546 PG 5 WC Entomology SC Entomology GA AI8NF UT WOS:000337173700048 PM 24772573 ER PT J AU Hribljan, JA Kane, ES Pypker, TG Chimner, RA AF Hribljan, John A. Kane, Evan S. Pypker, Thomas G. Chimner, Rodney A. TI The effect of long-term water table manipulations on dissolved organic carbon dynamics in a poor fen peatland SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Review DE peatland; dissolved organic carbon; residence time; climate change; water table ID PHENOL OXIDASE ACTIVITY; BIOGENIC GAS-BUBBLES; CLIMATE-CHANGE; CANADIAN PEATLANDS; BOREAL PEATLANDS; DOC PRODUCTION; MATTER DECOMPOSITION; NORTHERN PEATLAND; DRAINED PEATLAND; WETLAND SOILS AB Dissolved organic carbon (DOC) production, consumption, and quality displayed differences after long-term (similar to 55years) hydrological alterations in a poor fen peatland in northern Michigan. The construction of an earthen levee resulted in areas of a raised and lowered water table (WT) relative to an unaltered intermediate WT site. The lowered WT site had greater peat aeration and larger seasonal vertical WT fluctuations that likely elevated peat decomposition and subsidence with subsequent increases in bulk density, vertical hydraulic gradient, decreased hydraulic conductivity (K-sat), and a greater pore water residence time relative to the unaltered site. The raised WT site displayed a decreased K-sat combined with seasonal upwelling events that contributed to a longer residence time in comparison to the unaltered site. These differences are potentially contributing to elevated DOC concentrations at the lowered and raised WT site relative to the unaltered site. Additionally, spectrophotometric indices and chemical constituent assays indicated that the lowered site DOC was more aromatic and contained elevated concentrations of phenolics compared to the intermediate site. The raised site DOC was less aromatic, less humified, and also had a greater phenolic content than the intermediate site. Furthermore, an incubation experiment showed that DOC in the raised site contained the greatest labile carbon source. Based on our results, long-term WT alterations will likely impose significant effects on DOC dynamics in these peatlands; however, WT position alone was not a good predictor of DOC concentrations, though impoundment appears to produce a more labile DOC whereas drainage increases DOC aromaticity. C1 [Hribljan, John A.; Kane, Evan S.; Pypker, Thomas G.; Chimner, Rodney A.] Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. [Hribljan, John A.; Kane, Evan S.] US Forest Serv, No Res Stn, Houghton, MI USA. [Pypker, Thomas G.] Thompson Rivers Univ, Dept Nat Resource Sci, Kamloops, BC, Canada. RP Hribljan, JA (reprint author), Michigan Technol Univ, Sch Forest Resources & Environm Sci, Houghton, MI 49931 USA. EM jahriblj@mtu.edu FU U.S. Department of Energy's Office of Science (BER) through the Midwestern Regional Center of the National Institute for Climatic Change Research at Michigan Technological University; Ecosystem Science Center at Michigan Technological University; United States Forest Service Northern Research Station; Seney National Wildlife Refuge FX The authors thank Arielle Garrett, Elizabeth Boisvert, Chris Johnson, and Drew Ballantyne for help with site construction. We thank Arielle Garrett and Jim Bess for field assistance and Jennifer Eikenberry, Arielle Garrett, and Justina Silva for laboratory assistance and Merritt Turetsky for intellectual input on the study design. This research was supported by the U.S. Department of Energy's Office of Science (BER) through the Midwestern Regional Center of the National Institute for Climatic Change Research at Michigan Technological University. Additional support was provided by the Ecosystem Science Center at Michigan Technological University and the United States Forest Service Northern Research Station. Molly A. Cavaleri provided valuable comments on an earlier version of this manuscript. The project would not have been possible without the generous support of the Seney National Wildlife Refuge, in particular, R. Gregory Corace, III and Dave Olson, providing technical assistance and access to the research site. NR 120 TC 5 Z9 5 U1 8 U2 60 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD APR PY 2014 VL 119 IS 4 BP 577 EP 595 DI 10.1002/2013JG002527 PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AI9UN UT WOS:000337285500007 ER PT J AU Renninger, HJ Carlo, N Clark, KL Schafer, KVR AF Renninger, Heidi J. Carlo, Nicholas Clark, Kenneth L. Schaefer, Karina V. R. TI Modeling respiration from snags and coarse woody debris before and after an invasive gypsy moth disturbance SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE wood decay; mortality; modeling; gypsy moth; defoliation; oaks ID EASTERN UNITED-STATES; NEW-JERSEY PINELANDS; CLIMATE-CHANGE; FOREST DISTURBANCE; POTENTIAL CHANGES; TROPICAL FORESTS; CARBON BALANCE; CENTRAL AMAZON; NORTH-AMERICA; GLOBAL CHANGE AB Although snags and coarse woody debris are a small component of ecosystem respiration, disturbances can significantly increase the mass and respiration from these carbon (C) pools. The objectives of this study were to (1) measure respiration rates of snags and coarse woody debris throughout the year in a forest previously defoliated by gypsy moths, (2) develop models for dead stem respiration rates, (3) model stand-level respiration rates of dead stems using forest inventory and analysis data sets and environmental variables predisturbance and postdisturbance, and (4) compare total dead stem respiration rates with total ecosystem respiration and net ecosystem exchange. Respiration rates were measured on selected Pinus and Quercus snags and coarse woody debris each month for 1 year in a northeastern U.S. temperate forest. Multiple linear regression using environmental and biometric variables including wood temperature, diameter, density, species, and decay class was used to model respiration rates of dead stems. The mass of snags and coarse woody debris increased more than fivefold after disturbance and respiration rates increased more than threefold. The contribution of dead stems to total ecosystem respiration more than tripled from 0.85% to almost 3% and respiration from dead stems alone was approximately equal to the net ecosystem exchange of the forest in 2011 (fourth year postdisturbance). This study highlights the importance of dead stem C pools and fluxes particularly during disturbance and recovery cycles. With climate change increasing the ranges of many forest pests and pathogens, these data become particularly important for accurately modeling future C cycling. C1 [Renninger, Heidi J.; Schaefer, Karina V. R.] Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA. [Carlo, Nicholas; Schaefer, Karina V. R.] Rutgers State Univ, Dept Earth & Environm Sci, Newark, NJ 07102 USA. [Clark, Kenneth L.] US Forest Serv, USDA, No Res Stn, New Lisbon, NJ USA. RP Renninger, HJ (reprint author), Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA. EM hrenninger@gmail.com RI Renninger, Heidi/E-5237-2015 OI Renninger, Heidi/0000-0002-2485-9835 FU USDA Forest Service [10-JV-11242306-136]; Office of Science (BER), U.S. Department of Energy [DE-SC0007041] FX This work was funded by USDA Forest Service joint venture agreement 10-JV-11242306-136 and Office of Science (BER), U.S. Department of Energy DE-SC0007041 to KVRS. NR 67 TC 7 Z9 8 U1 2 U2 24 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD APR PY 2014 VL 119 IS 4 BP 630 EP 644 DI 10.1002/2013JG002542 PG 15 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AI9UN UT WOS:000337285500010 ER PT J AU Mo, C Kim, G Lee, K Kim, MS Cho, BK Lim, J Kang, S AF Mo, Changyeun Kim, Giyoung Lee, Kangjin Kim, Moon S. Cho, Byoung-Kwan Lim, Jongguk Kang, Sukwon TI Non-Destructive Quality Evaluation of Pepper (Capsicum annuum L.) Seeds Using LED-Induced Hyperspectral Reflectance Imaging SO SENSORS LA English DT Article DE hyperspectral imaging; pepper seed; quality; PLS-DA; LED ID FLUORESCENCE; SPECTROSCOPY; CHLOROPHYLL; SYSTEM; FRUIT AB In this study, we developed a viability evaluation method for pepper (Capsicum annuum L.) seeds based on hyperspectral reflectance imaging. The reflectance spectra of pepper seeds in the 400-700 nm range are collected from hyperspectral reflectance images obtained using blue, green, and red LED illumination. A partial least squares-discriminant analysis (PLS-DA) model is developed to classify viable and non-viable seeds. Four spectral ranges generated with four types of LEDs (blue, green, red, and RGB), which were pretreated using various methods, are investigated to develop the classification models. The optimal PLS-DA model based on the standard normal variate for RGB LED illumination (400-700 nm) yields discrimination accuracies of 96.7% and 99.4% for viable seeds and nonviable seeds, respectively. The use of images based on the PLS-DA model with the first-order derivative of a 31.5-nm gap for red LED illumination (600-700 nm) yields 100% discrimination accuracy for both viable and nonviable seeds. The results indicate that a hyperspectral imaging technique based on LED light can be potentially applied to high-quality pepper seed sorting. C1 [Mo, Changyeun; Kim, Giyoung; Lee, Kangjin; Lim, Jongguk; Kang, Sukwon] Rural Dev Adm, Natl Acad Agr Sci, Suwon 441100, Gyeonggi Do, South Korea. [Kim, Moon S.] ARS, Environm Microbiol & Food Safety Lab, BARC East, USDA, Beltsville, MD 20705 USA. [Cho, Byoung-Kwan] Chungnam Natl Univ, Dept Biosyst Machinery Engn, Coll Agr & Life Sci, Taejon 305764, South Korea. RP Kim, G (reprint author), Rural Dev Adm, Natl Acad Agr Sci, 150 Suinro, Suwon 441100, Gyeonggi Do, South Korea. EM cymoh100@korea.kr; giyoung@korea.kr; gadinlee@korea.kr; Moon.Kim@ars.usda.gov; chobk@cnu.ac.kr; limjg@korea.kr; skang@korea.kr FU "Research Program for Agricultural Science & Technology Development", National Academy of Agricultural Science, Rural Development Administration, Republic of Korea [PJ009399] FX This study was carried out with the support of "Research Program for Agricultural Science & Technology Development (Project No. PJ009399)", National Academy of Agricultural Science, Rural Development Administration, Republic of Korea. NR 21 TC 5 Z9 5 U1 7 U2 29 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1424-8220 J9 SENSORS-BASEL JI Sensors PD APR PY 2014 VL 14 IS 4 BP 7489 EP 7504 DI 10.3390/s140407489 PG 16 WC Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation SC Chemistry; Electrochemistry; Instruments & Instrumentation GA AI3TB UT WOS:000336784600094 PM 24763251 ER PT J AU Peiffer, JA Romay, MC Gore, MA Flint-Garcia, SA Zhang, ZW Millard, MJ Gardner, CAC McMullen, MD Holland, JB Bradbury, PJ Buckler, ES AF Peiffer, Jason A. Romay, Maria C. Gore, Michael A. Flint-Garcia, Sherry A. Zhang, Zhiwu Millard, Mark J. Gardner, Candice A. C. McMullen, Michael D. Holland, James B. Bradbury, Peter J. Buckler, Edward S. TI The Genetic Architecture Of Maize Height SO GENETICS LA English DT Article DE GBLUP; height; maize; plant ID ASSOCIATION MAPPING POPULATION; GENOME-WIDE ASSOCIATION; PLANT HEIGHT; GREEN-REVOLUTION; LINKAGE DISEQUILIBRIUM; QUANTITATIVE GENETICS; SELECTION; PREDICTION; ENZYME; INFORMATION AB Height is one of the most heritable and easily measured traits in maize (Zea mays L.). Given a pedigree or estimates of the genomic identity-by-state among related plants, height is also accurately predictable. But, mapping alleles explaining natural variation in maize height remains a formidable challenge. To address this challenge, we measured the plant height, ear height, flowering time, and node counts of plants grown in >64,500 plots across 13 environments. These plots contained >7300 inbreds representing most publically available maize inbreds in the United States and families of the maize Nested Association Mapping (NAM) panel. Joint-linkage mapping of quantitative trait loci (QTL), fine mapping in near isogenic lines (NILs), genome-wide association studies (GWAS), and genomic best linear unbiased prediction (GBLUP) were performed. The heritability of maize height was estimated to be >90%. Mapping NAM family-nested QTL revealed the largest explained 2.1 +/- 0.9% of height variation. The effects of two tropical alleles at this QTL were independently validated by fine mapping in NIL families. Several significant associations found by GWAS colocalized with established height loci, including brassinosteroid-deficient dwarf1, dwarf plant1, and semi-dwarf2. GBLUP explained >80% of height variation in the panels and outperformed bootstrap aggregation of family-nested QTL models in evaluations of prediction accuracy. These results revealed maize height was under strong genetic control and had a highly polygenic genetic architecture. They also showed that multiple models of genetic architecture differing in polygenicity and effect sizes can plausibly explain a population's variation in maize height, but they may vary in predictive efficacy. C1 [Peiffer, Jason A.] N Carolina State Univ, Bioinformat Res Ctr, Dept Genet, Raleigh, NC 27695 USA. [Romay, Maria C.; Zhang, Zhiwu; Buckler, Edward S.] Cornell Univ, Inst Genom Div, Ithaca, NY 14853 USA. [Gore, Michael A.; Buckler, Edward S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Flint-Garcia, Sherry A.; McMullen, Michael D.] USDA ARS, Columbia, MO 65211 USA. [Flint-Garcia, Sherry A.; McMullen, Michael D.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Millard, Mark J.; Gardner, Candice A. C.] USDA ARS, Ames, IA 50011 USA. [Millard, Mark J.; Gardner, Candice A. C.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Holland, James B.] USDA ARS, Raleigh, NC 27695 USA. [Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Bradbury, Peter J.; Buckler, Edward S.] USDA ARS, Ithaca, NY 14853 USA. RP Peiffer, JA (reprint author), N Carolina State Univ, Bioinformat Res Ctr, Raleigh, NC 27695 USA. EM japeiffe@ncsu.edu RI Romay, Maria/B-8831-2015; OI Romay, Maria/0000-0001-9309-1586; Buckler, Edward/0000-0002-3100-371X; Zhang, Zhiwu/0000-0002-5784-9684; Holland, James/0000-0002-4341-9675 NR 71 TC 54 Z9 57 U1 13 U2 99 PU GENETICS SOC AM PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD APR PY 2014 VL 196 IS 4 BP 1337 EP + DI 10.1534/genetics.113.159152 PG 22 WC Genetics & Heredity SC Genetics & Heredity GA AE7LL UT WOS:000334179300035 PM 24514905 ER PT J AU Liu, SS Liu, YB AF Liu, Samuel S. Liu, Yong-Biao TI Reducing Injury of Lettuce from Phosphine Fumigation SO HORTTECHNOLOGY LA English DT Article DE CO2 absorbent; Lactuca sativa; postharvest quality; quarantine treatment ID CARBON-DIOXIDE; STORAGE LIFE; DISINFESTATION; RESISTANCE; TOXICITY; QUALITY AB Fumigation with cylindered pure phosphine free of ammonia has been used commercially at low temperatures in recent years to control pests on harvested fresh fruit and vegetables. However, long fumigation treatments cause injuries to lettuce (Lactuca sativa) and prevent its commercial use on lettuce. We evaluated whether absorbents for carbon dioxide (CO2) and ethylene can prevent injuries to lettuce in phosphine fumigation, hypothesizing that an accumulation of CO2 or ethylene be responsible for the injuries. Head and romaine lettuce were fumigated in chambers in the presence or absence of CO2 and ethylene absorbents for 3 days at 2 degrees C. The use of absorbents prevented lettuce injury associated with fumigation and resulted in higher lettuce quality. In the absence of the absorbents, both head and romaine lettuce sustained significant injuries in the form of brown stain, a typical symptom of CO2 injuries, and significantly reduced quality as compared with fumigation in the presence of absorbents. The injuries were likely caused by CO2 based on the facts that injuries were identical to CO2 injuries and the prevention of the injuries by the use of CO2 absorbent. The findings of this study have important implications for developing safe and effective phosphine fumigation protocols at low temperature for controlling insect pests on fresh commodities, especially when a long treatment time is required. C1 [Liu, Samuel S.; Liu, Yong-Biao] USDA ARS, Salinas, CA 93905 USA. RP Liu, YB (reprint author), USDA ARS, 1636 E Alisal St, Salinas, CA 93905 USA. EM yongbiao.liu@ars.usda.gov FU Technical Assistance for Specialty Crop program of U.S. Department of Agriculture (USDA), Foreign Agricultural Service FX The authors would like to thank T. Masuda and J. Wasson for technical assistance. We thank Tanimura & Antic Fresh Foods (Salinas, CA) for providing lettuce. This study was supported by a Technical Assistance for Specialty Crop program of U.S. Department of Agriculture (USDA), Foreign Agricultural Service. NR 32 TC 1 Z9 1 U1 1 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD APR PY 2014 VL 24 IS 2 BP 188 EP 195 PG 8 WC Horticulture SC Agriculture GA AI8WZ UT WOS:000337209000005 ER PT J AU Jong, L AF Jong, Lei TI Modulus enhancement of natural rubber through the dispersion size reduction of protein/fiber aggregates SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Natural rubber; Mechanical properties; Filled rubber; Polymer-filler interactions ID FILLER INTERACTIONS; COMPOSITES; STARCH; NANOCRYSTALS; BEHAVIOR AB Improved mechanical properties of natural rubber are required for various rubber applications. Aggregates of protein and fiber that constitute soy protein concentrate were shear-reduced and used to enhance the tensile modulus of the natural rubber. The aqueous dispersion of the shear-reduced aggregates has a higher viscosity and shear storage modulus. The reduced aggregates in the natural rubber increased the tensile modulus of the natural rubber significantly. Compared to the natural rubber, the tensile modulus of 30% filled rubber increased 10-fold at 200% elongation. The frequency dependent shear modulus, swelling experiments, and dynamic strain sweep experiments were used to analyze polymer-filler interactions. The result shows that the polymer-filler interaction is small and is not the dominant factor that enhances the tensile properties of the natural rubber. Instead, the filler-filler network is the cause for the mechanical enhancement of the natural rubber. Published by Elsevier B.V. C1 USDA ARS, Natl Ctr Agr Utilizat Res, Dept Agr, Peoria, IL 61604 USA. RP Jong, L (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Dept Agr, 1815 N Univ St, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 30 TC 3 Z9 3 U1 1 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2014 VL 55 BP 25 EP 32 DI 10.1016/j.indcrop.2014.01.057 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA AI6WL UT WOS:000337017600004 ER PT J AU Sanchez, PL Chen, MK Pessarakli, M Hill, HJ Gore, MA Jenks, MA AF Sanchez, Paul L. Chen, Mei-kuang Pessarakli, Mohammad Hill, Hank J. Gore, Michael A. Jenks, Matthew A. TI Effects of temperature and salinity on germination of non-pelleted and pelleted guayule (Parthenium argentatum A. Gray) seeds SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Salt stress; Temperature response; Viability; Natural rubber; Parthenium argentatum ID EARLY SEEDLING GROWTH; SALT STRESS; ESTABLISHMENT; RESPONSES; DORMANCY; QUALITY; L. AB Guayule (Parthenium argentatum A. Gray) is an important domestic source of natural rubber. Commercial field plots are currently established using greenhouse grown seedlings that are hand sown as plugs. However, this practice is expensive and laborious. Direct sowing of guayule seed in the field would reduce time and cost significantly, and yet the effects of seed pelleting, temperature, salinity level, and their interactions on guayule seed germination are not well established. To test germination requirements, non-pelleted (control) and pelleted seeds were planted in solutions having electrical conductivity (EC) of 0, 2, 4, 6, 8, and 10 mS/cm at 10, 20, 30, and 40 degrees C for 7 days. After 7 days, the non-germinated seeds were transferred to distilled water plates in a 20 degrees C environment. Seed pelleting, temperature, salinity, and their interactions significantly affected guayule germination. The optimal conditions for seed germination (i.e., highest germination rates) were found to be 20 degrees C and EC 0-2 mS/cm, regardless of pelleting. Both temperature and salinity delayed germination and decreased viability. Germination was inhibited at both 10 and 40 degrees C. Salinity effects on seeds decreased as germination temperature became optimal. Lowest germination percentages were observed at EC 6-10 mS/cm and at 30 and 40 degrees C. Germination percentages increased for treatments after seeds were transferred to optimal conditions. Importantly, pelleted guayule seeds exhibited higher germination than non-pelleted seeds in all treatments. Our results provide important new insights that can help guide the selection of optimal seasonal and soil conditions for field establishment with new direct seeding methods. Published by Elsevier B.V. C1 [Sanchez, Paul L.; Jenks, Matthew A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. [Chen, Mei-kuang] Univ Arizona, Dept Teaching Learning & Sociocultural Studies, Tucson, AZ 85721 USA. [Pessarakli, Mohammad] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Hill, Hank J.] Seed Dynam Inc, Salinas, CA 93901 USA. [Gore, Michael A.] Cornell Univ, Ithaca, NY 14853 USA. RP Jenks, MA (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM paul.sanchez@ars.usda.gov; kuang@email.arizona.edu; pessarak@email.arizona.edu; hankh@seeddynamics.com; mag87@cornell.edu; matt.jenks@ars.usda.gov OI Chen, Mei-kuang/0000-0002-0407-6788 FU USDA-ARS; USDA-NIFA/DOE Biomass Research and Development Initiative (BRDI) [2012-10006] FX We wish to thank Drs. Patricia Mazzarella, Lee Sechrest, and Elsa Rubia-Sanchez for editorial contributions, and Seth Weakly for technical assistance. This work was supported by USDA-ARS and the USDA-NIFA/DOE Biomass Research and Development Initiative (BRDI) Grant No. 2012-10006. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 45 TC 2 Z9 3 U1 2 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2014 VL 55 BP 90 EP 96 DI 10.1016/j.indcrop.2014.01.050 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA AI6WL UT WOS:000337017600013 ER PT J AU Hojilla-Evangelista, MP Selling, GW Berhow, MA Evangelista, RL AF Hojilla-Evangelista, Mila P. Selling, Gordon W. Berhow, Mark A. Evangelista, Roque L. TI Preparation, composition and functional properties of pennycress (Thlaspi arvense L.) seed protein isolates SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Pennycress; Pennycress protein isolates; Protein extraction; Protein functionality ID CHEMICAL-COMPOSITION; CANOLA PROTEINS; PRESS CAKE; EXTRACTION; ULTRAFILTRATION; OIL; SOLUBILITY; COOKING; PROFILE; MEALS AB This study evaluated two methods, saline extraction (SE) and conventional acid precipitation (AP), to recover proteins from pennycress (Thlaspi arvense L) seed meal. SE was done using 0.1 M NaCl at 50 degrees C while AP involved alkaline extraction (pH 10) first followed by protein precipitation at pH 4. Composition, amino acid profiles, and functional properties (solubility, foaming, emulsification, water-holding capacity, heat coagulability) of the resultant protein extracts were compared. SE and AP produced pennycress protein extracts that were sinigrin-free and containing at least 90% (db) crude protein, which classifies the extracts as protein isolates (PI). Extraction method had major influence on the amino acid profiles and functional properties of the protein isolates. Pennycress SEPI was markedly more soluble (68-91% solubility at pH 2 and >= 7) and had excellent emulsifying properties that were clearly superior to those of APPI. On the other hand, APPI had better foaming properties and was more stable to heating than SEPI. These results strongly demonstrate that high-purity pennycress seed protein isolates can be produced by either saline extraction or acid precipitation and have functional properties that are desirable for non-food uses. Published by Elsevier B.V. C1 [Hojilla-Evangelista, Mila P.; Selling, Gordon W.] USDA ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Berhow, Mark A.] USDA ARS, Funct Foods Res Unit, NCAUR, Peoria, IL 61604 USA. [Evangelista, Roque L.] USDA ARS, Biooils Res Unit, NCAUR, Peoria, IL 61604 USA. RP Hojilla-Evangelista, MP (reprint author), USDA ARS, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Mila.HojillaEvangelista@ars.usda.gov NR 40 TC 4 Z9 4 U1 1 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD APR PY 2014 VL 55 BP 173 EP 179 DI 10.1016/j.indcrop.2014.02.016 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA AI6WL UT WOS:000337017600024 ER PT J AU Fettig, CJ Gibson, KE Munson, AS Negron, JF AF Fettig, Christopher J. Gibson, Kenneth E. Munson, A. Steven Negron, Jose F. TI A Comment on "Management for Mountain Pine Beetle Outbreak Suppression: Does Relevant Science Support Current Policy?" SO FORESTS LA English DT Article DE Dendroctonus ponderosae; direct control; indirect control; Pinus contorta; Pinus ponderosa; sanitation; thinning ID FUEL-REDUCTION TREATMENTS; UNITED-STATES AB There are two general approaches for reducing the negative impacts of mountain pine beetle, Dendroctonus ponderosae Hopkins, on forests. Direct control involves short-term tactics designed to address current infestations by manipulating mountain pine beetle populations, and includes the use of fire, insecticides, semiochemicals, sanitation harvests, or a combination of these treatments. Indirect control is preventive, and designed to reduce the probability and severity of future infestations within treated areas by manipulating stand, forest and/or landscape conditions by reducing the number of susceptible host trees through thinning, prescribed burning, and/or alterations of age classes and species composition. We emphasize that. outbreak suppression. is not the intent or objective of management strategies implemented for mountain pine beetle in the western United States, and that the use of clear, descriptive language is important when assessing the merits of various treatment strategies. C1 [Fettig, Christopher J.] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Gibson, Kenneth E.] US Forest Serv, USDA, Missoula, MT 59807 USA. [Munson, A. Steven] US Forest Serv, USDA, Ogden, UT 84403 USA. [Negron, Jose F.] US Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO 80526 USA. RP Fettig, CJ (reprint author), US Forest Serv, Pacific Southwest Res Stn, USDA, 1731 Res Pk Dr, Davis, CA 95618 USA. EM cfettig@fs.fed.us; keg_a1@bresnan.net; smunson@fs.fed.us; jnegron@fs.fed.us NR 17 TC 2 Z9 2 U1 1 U2 12 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1999-4907 J9 FORESTS JI Forests PD APR PY 2014 VL 5 IS 4 BP 822 EP 826 DI 10.3390/f5040822 PG 5 WC Forestry SC Forestry GA AI5KL UT WOS:000336905000013 ER PT J AU Kiefer, MT Heilman, WE Zhong, SY Charney, JJ Bian, XD Skowronski, NS Hom, JL Clark, KL Patterson, M Gallagher, MR AF Kiefer, Michael T. Heilman, Warren E. Zhong, Shiyuan Charney, Joseph J. Bian, Xindi Skowronski, Nicholas S. Hom, John L. Clark, Kenneth L. Patterson, Matthew Gallagher, Michael R. TI Multiscale Simulation of a Prescribed Fire Event in the New Jersey Pine Barrens Using ARPS-CANOPY SO JOURNAL OF APPLIED METEOROLOGY AND CLIMATOLOGY LA English DT Article ID LARGE-EDDY SIMULATION; PREDICTION SYSTEM ARPS; BOUNDARY-LAYER; SOLAR-RADIATION; MODEL; FLOW; TRANSPORT; MOUNTAIN; PARAMETERIZATION; COORDINATE AB Smoke prediction products are one of the tools used by land management personnel for decision making regarding prescribed fires. This study documents the application to a prescribed fire of a smoke prediction system that employs ARPS-CANOPY, a modified version of the Advanced Regional Prediction System (ARPS) model containing a canopy submodel, as the meteorological driver. In this paper, the performance of ARPS-CANOPY in simulating meteorological fields in the vicinity of a low-intensity fire is assessed using flux-tower data collected prior to and during a low-intensity prescribed fire in the New Jersey Pine Barrens in March 2011. A three-dimensional high-resolution plant area density dataset is utilized to define the characteristics of the canopy, and the fire is represented in ARPS-CANOPY as a heat flux to the atmosphere. The standard ARPS model is compared with reanalysis and upper-air data to establish that the model can simulate the observed synoptic-mesoscale and planetary boundary layer features that are salient to this study. ARPS-CANOPY profiles of mean turbulent kinetic energy, wind speed/direction, and temperature exhibit patterns that appear in the flux-tower observations during both the preburn phase of the experiment and the period of time the flux tower experienced perturbed atmospheric conditions due to the impinging fire. Last, the character and source of turbulence in and around the fire line are examined. These results are encouraging for smoke prediction efforts since transport of smoke from low-intensity fires is highly sensitive to the near-surface meteorological conditions and, in particular, turbulent flows. C1 [Kiefer, Michael T.; Zhong, Shiyuan] Michigan State Univ, Dept Geog, E Lansing, MI 48824 USA. [Heilman, Warren E.; Charney, Joseph J.; Bian, Xindi] US Forest Serv, No Res Stn, USDA, E Lansing, MI USA. [Skowronski, Nicholas S.] US Forest Serv, No Res Stn, USDA, Morgantown, WV USA. [Hom, John L.; Patterson, Matthew] US Forest Serv, No Res Stn, USDA, Newtown Sq, PA USA. [Clark, Kenneth L.; Gallagher, Michael R.] US Forest Serv, No Res Stn, USDA, New Lisbon, NJ USA. RP Kiefer, MT (reprint author), Michigan State Univ, Geog Bldg Rm 203,673 Auditorium Rd, E Lansing, MI 48824 USA. EM mtkiefer@msu.edu RI Skowronski, Nicholas/N-4417-2013; OI Skowronski, Nicholas/0000-0002-5801-5614; Kiefer, Michael/0000-0002-4824-0148 FU U.S. Joint Fire Science Program [09-1-04-1]; USDA Forest Service [09-JV-11242306-089] FX Support for this research was provided by the U.S. Joint Fire Science Program (Project 09-1-04-1) and the USDA Forest Service (Research Joint Venture Agreement 09-JV-11242306-089). Special thanks are given to the N. J. Forest Fire Service for conducting the burn and Bob Kremens for use of the infrared imagery of the fireline. We also thank Alan Srock, Jovanka Nicolic, and three anonymous reviewers for providing helpful comments and suggestions to improve the manuscript. NR 39 TC 4 Z9 4 U1 0 U2 12 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1558-8424 EI 1558-8432 J9 J APPL METEOROL CLIM JI J. Appl. Meteorol. Climatol. PD APR PY 2014 VL 53 IS 4 BP 793 EP 812 DI 10.1175/JAMC-D-13-0131.1 PG 20 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA AH9GG UT WOS:000336449300001 ER PT J AU Neri, FM Cook, AR Gibson, GJ Gottwald, TR Gilligan, CA AF Neri, Franco M. Cook, Alex R. Gibson, Gavin J. Gottwald, Tim R. Gilligan, Christopher A. TI Bayesian Analysis for Inference of an Emerging Epidemic: Citrus Canker in Urban Landscapes SO PLOS COMPUTATIONAL BIOLOGY LA English DT Article ID DROUGHT-INDUCED AMPLIFICATION; MOUTH-DISEASE EPIDEMIC; INFECTIOUS-DISEASES; GREAT-BRITAIN; CLIMATE-CHANGE; STOCHASTIC-MODELS; VECTOR-BORNE; 2001 FOOT; SPREAD; INFLUENZA AB Outbreaks of infectious diseases require a rapid response from policy makers. The choice of an adequate level of response relies upon available knowledge of the spatial and temporal parameters governing pathogen spread, affecting, amongst others, the predicted severity of the epidemic. Yet, when a new pathogen is introduced into an alien environment, such information is often lacking or of no use, and epidemiological parameters must be estimated from the first observations of the epidemic. This poses a challenge to epidemiologists: how quickly can the parameters of an emerging disease be estimated? How soon can the future progress of the epidemic be reliably predicted? We investigate these issues using a unique, spatially and temporally resolved dataset for the invasion of a plant disease, Asiatic citrus canker in urban Miami. We use epidemiological models, Bayesian Markov-chain Monte Carlo, and advanced spatial statistical methods to analyse rates and extent of spread of the disease. A rich and complex epidemic behaviour is revealed. The spatial scale of spread is approximately constant over time and can be estimated rapidly with great precision (although the evidence for long-range transmission is inconclusive). In contrast, the rate of infection is characterised by strong monthly fluctuations that we associate with extreme weather events. Uninformed predictions from the early stages of the epidemic, assuming complete ignorance of the future environmental drivers, fail because of the unpredictable variability of the infection rate. Conversely, predictions improve dramatically if we assume prior knowledge of either the main environmental trend, or the main environmental events. A contrast emerges between the high detail attained by modelling in the spatiotemporal description of the epidemic and the bottleneck imposed on epidemic prediction by the limits of meteorological predictability. We argue that identifying such bottlenecks will be a fundamental step in future modelling of weather-driven epidemics. Author Summary We consider emerging epidemics, arising, e.g., when a new pathogen is introduced in a host population. In face of the new threat, crucial control measures have to be implemented quickly, yet prior knowledge of the parameters underlying pathogen spread and transmission is often missing. Predictive modelling can greatly help in informing decision making by estimating those parameters from early observations of the outbreak. The important questions are then: can a modeller characterise the disease soon enough, i.e., within a useful time frame, in order to enact the proper control measures? At what stage of the outbreak can the future epidemic progress be reliably predicted? We analyse an outbreak of citrus canker, a wind-spread bacterial disease of citrus, in urban Miami. The model succeeds in capturing the main epidemiological features of the disease, but we find contrasting answers. The spatial scale of disease spread can be identified quickly and accurately from early observations. However, the rate of spread is rapidly changing in time, driven mainly by rare thunderstorms with very short-time predictability, which frustrates epidemic prediction. C1 [Neri, Franco M.; Gilligan, Christopher A.] Univ Cambridge, Dept Plant Sci, Cambridge, England. [Cook, Alex R.] Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore 117548, Singapore. [Cook, Alex R.] Natl Univ Hlth Syst, Singapore, Singapore. [Cook, Alex R.] Natl Univ Singapore, Dept Stat & Appl Probabil, Singapore 117548, Singapore. [Cook, Alex R.] Duke NUS Grad Med Sch Singapore, Program Hlth Serv & Syst Res, Singapore, Singapore. [Cook, Alex R.] Tan Tock Seng Hosp, Communicable Dis Ctr, Singapore, Singapore. [Gibson, Gavin J.] Heriot Watt Univ, Dept Actuarial Math & Stat, Edinburgh, Midlothian, Scotland. [Gibson, Gavin J.] Heriot Watt Univ, Maxwell Inst Math Sci, Edinburgh, Midlothian, Scotland. [Gottwald, Tim R.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL USA. RP Neri, FM (reprint author), Univ Cambridge, Dept Plant Sci, Cambridge, England. EM fmn22@cam.ac.uk FU BBSRC; USDA-ARS; USDA-Aphis PPQ; Citrus Research and Development Foundation; USDA; Defra; National University of Singapore; NMRC FX FMN gratefully acknowledges financial support from BBSRC, USDA-ARS, USDA-Aphis PPQ, Citrus Research and Development Foundation. CAG gratefully acknowledges the support of a BBSRC Professorial Fellowship, with additional support from USDA and Defra. ARC was supported by BBSRC, USDA, the National University of Singapore, and NMRC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 70 TC 8 Z9 8 U1 4 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-734X EI 1553-7358 J9 PLOS COMPUT BIOL JI PLoS Comput. Biol. PD APR PY 2014 VL 10 IS 4 AR e1003587 DI 10.1371/journal.pcbi.1003587 PG 16 WC Biochemical Research Methods; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Mathematical & Computational Biology GA AI0AF UT WOS:000336507500005 PM 24762851 ER PT J AU Konstantinov, AS Linzmeier, AM Savini, VP AF Konstantinov, Alexander S. Linzmeier, Adelita M. Savini, Vilma P. TI STEVENALTICA, A NEW GENUS OF MOSS AND LEAF-LITTER INHABITING FLEA BEETLES FROM BOLIVIA (COLEOPTERA: CHRYSOMELIDAE: GALERUCINAE: ALTICINI). SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Article DE Bryobionts; key to genera; Neotropics AB A new genus, Stevenaltica, with two new species, S. normi and S. erronis, from Bolivia is described and illustrated. It is similar to Exoceras Jacoby. An identification key for all flea beetle genera known to occur in mosses in the Western Hemisphere is provided. C1 [Konstantinov, Alexander S.] USDA ARS, Smithsonian Inst, Natl Museum Nat Hist, Systemat Entomol Lab, Washington, DC 20013 USA. [Linzmeier, Adelita M.] Fundacao Univ Fed Grande Dourados, Fac Ciencias Biol & Ambientais, BR-79804980 Dourados, Mato Grosso Sul, Brazil. [Savini, Vilma P.] Cent Univ Venezuela, Fac Agron, Museo Inst Zool Agr, Maracay 2101 A, Estado Aragua, Venezuela. RP Konstantinov, AS (reprint author), USDA ARS, Smithsonian Inst, Natl Museum Nat Hist, Systemat Entomol Lab, POB 37012, Washington, DC 20013 USA. EM alex.konstantinov@ars.usda.gov; alinzmeier@yahoo.com.br; vsgioia@gmail.com RI caputo, carlo/N-3747-2014 NR 14 TC 0 Z9 0 U1 1 U2 4 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2014 VL 116 IS 2 BP 159 EP 171 DI 10.4289/0013-8797.116.2.159 PG 13 WC Entomology SC Entomology GA AI2BG UT WOS:000336661200003 ER PT J AU Malagon-Aldana, LA Serna, F Smith, AR AF Malagon-Aldana, Leonardo Andres Serna, Francisco Smith, Anddavid R. TI Siricidae (Hymenoptera) in Colombia, the first report of Urocerus gigas (Linnaeus) from northern South America SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Editorial Material C1 [Malagon-Aldana, Leonardo Andres; Serna, Francisco] Univ Nacl Colombia, UNAB, Fac Ciencias Agr, Museo Entomol, Bogota, DC, Colombia. [Smith, Anddavid R.] USDA ARS, Smithsonian Inst, Systemat Entomol Lab, Natl Museum Nat Hist, Washington, DC 20013 USA. RP Malagon-Aldana, LA (reprint author), Univ Nacl Colombia, UNAB, Fac Ciencias Agr, Museo Entomol, Cra 30 45-03, Bogota, DC, Colombia. EM lamalagona@unal.edu.co; fjsernac@unal.edu.co; sawfly2@aol.com NR 3 TC 1 Z9 1 U1 0 U2 0 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2014 VL 116 IS 2 BP 191 EP 192 DI 10.4289/0013-8797.116.2.191 PG 2 WC Entomology SC Entomology GA AI2BG UT WOS:000336661200006 ER PT J AU McKamey, SH Xue, QQ Viraktamath, CA Zhang, YL AF McKamey, Stuart H. Xue, Qingquan Viraktamath, Chandra A. Zhang, Yalin TI New Name for One and Fixation of Correct Spelling of Two Leafhopper Taxa (Hemiptera: Cicadellidae) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Editorial Material C1 [McKamey, Stuart H.] USDA ARS, Smithsonian Inst, Systemat Entomol Lab, Natl Museum Nat Hist, Washington, DC 20560 USA. [Xue, Qingquan; Zhang, Yalin] Northwest A&F Univ, Entomol Museum, Yangling 712100, Shaanxi, Peoples R China. [Viraktamath, Chandra A.] Univ Agr Sci, GKVK, Dept Entomol, Bangalore 560065, Karnataka, India. RP McKamey, SH (reprint author), USDA ARS, Smithsonian Inst, Systemat Entomol Lab, Natl Museum Nat Hist, MRC 168,10th & Constitut Ave, Washington, DC 20560 USA. EM smckamey@ars.usda.gov NR 5 TC 0 Z9 0 U1 0 U2 3 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2014 VL 116 IS 2 BP 193 EP 194 DI 10.4289/0013-8797.116.2.193 PG 2 WC Entomology SC Entomology GA AI2BG UT WOS:000336661200007 ER PT J AU Smith, DR Williams, G AF Smith, David R. Williams, Gaye TI Waldheimia carbonaria (Cresson) (Hymenoptera: Tenthredinidae), a sawfly on Missouri primrose, Oenothera missouriensis Sims (Onagraceae) SO PROCEEDINGS OF THE ENTOMOLOGICAL SOCIETY OF WASHINGTON LA English DT Editorial Material C1 [Smith, David R.] USDA ARS, Smithsonian Inst, Natl Museum Nat Hist, Dept Agr,Systemat Entomol Lab, Washington, DC 20013 USA. [Williams, Gaye] Maryland Dept Agr, Plant Protect & Weed Management Sect, Annapolis, MD 21401 USA. RP Smith, DR (reprint author), USDA ARS, Smithsonian Inst, Natl Museum Nat Hist, Dept Agr,Systemat Entomol Lab, POB 37012,MRC 168, Washington, DC 20013 USA. EM sawfly2@aol.com; gaye.williams@maryland.gov NR 4 TC 3 Z9 3 U1 0 U2 2 PU ENTOMOL SOC WASHINGTON PI WASHINGTON PA SMITHSONIAN INSTITUTION DEPT ENTOMOLOGY, WASHINGTON, DC 20560 USA SN 0013-8797 J9 P ENTOMOL SOC WASH JI Proc. Entomol. Soc. Wash. PD APR PY 2014 VL 116 IS 2 BP 197 EP 198 DI 10.4289/0013-8797.116.2.197 PG 2 WC Entomology SC Entomology GA AI2BG UT WOS:000336661200009 ER PT J AU Muths, E Bailey, LL Watry, MK AF Muths, Erin Bailey, Larissa L. Watry, Mary Kay TI Animal reintroductions: An innovative assessment of survival SO BIOLOGICAL CONSERVATION LA English DT Article DE Adaptive management; Anaxyrus boreas; Rocky Mountain National Park; Multistate robust design ID TOADS ANAXYRUS-BOREAS; BATRACHOCHYTRIUM-DENDROBATIDIS; BUFO-BOREAS; AMPHIBIAN POPULATIONS; CONSERVATION; DISEASE; CHYTRIDIOMYCOSIS; RECRUITMENT; DISPERSAL; DECLINE AB Quantitative evaluations of reintroductions are infrequent and assessments of milestones reached before a project is completed, or abandoned due to lack of funding, are rare. However, such assessments, which are promoted in adaptive management frameworks, are critical. Quantification can provide defensible estimates of biological success, such as the number of survivors from a released cohort, with associated cost per animal. It is unlikely that the global issues of endangered wildlife and population declines will abate, therefore, assurance colonies and reintroductions are likely to become more common. If such endeavors are to be successful biologically or achieve adequate funding, implementation must be more rigorous and accountable. We use a novel application of a multistate, robust design capture-recapture model to estimate survival of reintroduced tadpoles through metamorphosis (i.e., the number of individuals emerging from the pond) and thereby provide a quantitative measure of effort and success for an "in progress" reintroduction of toads. Our data also suggest that tadpoles released at later developmental stages have an increased probability of survival and that eggs laid in the wild hatched at higher rates than eggs laid by captive toads. We illustrate how an interim assessment can identify problems, highlight successes, and provide information for use in adjusting the effort or implementing a Decision-Theoretic adaptive management strategy. Published by Elsevier Ltd. C1 [Muths, Erin] US Forest Serv, Ft Collins Sci Ctr, Ft Collins, CO 80526 USA. [Bailey, Larissa L.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Watry, Mary Kay] Rocky Mt Natl Pk, Estes Pk, CO 80517 USA. RP Muths, E (reprint author), US Forest Serv, Ft Collins Sci Ctr, 2150 Ctr Ave Bldg C, Ft Collins, CO 80526 USA. EM erin_muths@usgs.gov RI Bailey, Larissa/A-2565-2009 NR 73 TC 10 Z9 10 U1 8 U2 71 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0006-3207 EI 1873-2917 J9 BIOL CONSERV JI Biol. Conserv. PD APR PY 2014 VL 172 BP 200 EP 208 DI 10.1016/j.biocon.2014.02.034 PG 9 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AH7TK UT WOS:000336337800024 ER PT J AU Hansen, AJ Piekielek, N Davis, C Haas, J Theobald, DM Gross, JE Monahan, WB Olliff, T Running, SW AF Hansen, Andrew J. Piekielek, Nathan Davis, Cory Haas, Jessica Theobald, David M. Gross, John E. Monahan, William B. Olliff, Tom Running, Steven W. TI Exposure of U. S. National Parks to land use and climate change 1900-2100 SO ECOLOGICAL APPLICATIONS LA English DT Article DE climate change; policy; U; S; National Parks; vulnerability assessment ID PROTECTED AREAS; UNITED-STATES; CONSERVATION; MANAGEMENT; BIODIVERSITY; GROWTH; FRAGMENTATION; TEMPERATURE; ECOSYSTEMS; DROUGHT AB Many protected areas may not be adequately safeguarding biodiversity from human activities on surrounding lands and global change. The magnitude of such change agents and the sensitivity of ecosystems to these agents vary among protected areas. Thus, there is a need to assess vulnerability across networks of protected areas to determine those most at risk and to lay the basis for developing effective adaptation strategies. We conducted an assessment of exposure of U.S. National Parks to climate and land use change and consequences for vegetation communities. We first defined park protected-area centered ecosystems (PACEs) based on ecological principles. We then drew on existing land use, invasive species, climate, and biome data sets and models to quantify exposure of PACEs from 1900 through 2100. Most PACEs experienced substantial change over the 20th century (>740% average increase in housing density since 1940, 13% of vascular plants are presently nonnative, temperature increase of 1 degrees C/100 yr since 1895 in 80% of PACEs), and projections suggest that many of these trends will continue at similar or increasingly greater rates (255% increase in housing density by 2100, temperature increase of 2.5 degrees-4.5 degrees C/100 yr, 30% of PACE areas may lose their current biomes by 2030). In the coming century, housing densities are projected to increase in PACEs at about 82% of the rate of since 1940. The rate of climate warming in the coming century is projected to be 2.5-5.8 times higher than that measured in the past century. Underlying these averages, exposure of individual park PACEs to change agents differ in important ways. For example, parks such as Great Smoky Mountains exhibit high land use and low climate exposure, others such as Great Sand Dunes exhibit low land use and high climate exposure, and a few such as Point Reyes exhibit high exposure on both axes. The cumulative and synergistic effects of such changes in land use, invasives, and climate are expected to dramatically impact ecosystem function and biodiversity in national parks. These results are foundational to developing effective adaptation strategies and suggest policies to better safeguard parks under broad-scale environmental change. C1 [Hansen, Andrew J.; Piekielek, Nathan] Montana State Univ, Dept Ecol, Bozeman, MT 59717 USA. [Davis, Cory; Running, Steven W.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. [Haas, Jessica] US Forest Serv, USDA, Mt Res Stn, Missoula, MT 59807 USA. [Theobald, David M.; Gross, John E.; Monahan, William B.] Natl Pk Serv, Inventory & Monitoring Div, Ft Collins, CO 80525 USA. [Olliff, Tom] USGS Northern Rocky Mt Sci Ctr, Bozeman, MT 59715 USA. RP Hansen, AJ (reprint author), Montana State Univ, Dept Ecol, Bozeman, MT 59717 USA. EM hansen@montana.edu OI Piekielek, Nathan/0000-0002-2740-0675 FU NASA [BIOCLIM10-0034]; NASA Land Cover Land Use Change Program FX Funding was provided by the NASA Applied Sciences Program (10-BIOCLIM10-0034) and the NASA Land Cover Land Use Change Program. Data were provided by Gerald Rehfeldt, USDA Forest Sciences Lab, Moscow, Idaho. Robert Bennetts drafted Fig. 1. Comments by Gary Machlis improved the manuscript. NR 65 TC 22 Z9 22 U1 15 U2 83 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD APR PY 2014 VL 24 IS 3 BP 484 EP 502 DI 10.1890/13-0905.1 PG 19 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AD4TA UT WOS:000333242300006 PM 24834735 ER PT J AU Scheer, C Del Grosso, SJ Parton, WJ Rowlings, DW Grace, PR AF Scheer, Clemens Del Grosso, Stephen J. Parton, William J. Rowlings, David W. Grace, Peter R. TI Modeling nitrous oxide emissions from irrigated agriculture: testing DayCent with high-frequency measurements SO ECOLOGICAL APPLICATIONS LA English DT Article DE cotton-wheat cropping; DayCent; fertilizer N application rate; irrigation intensity; N2O emissions; simulation ID SOIL ORGANIC-MATTER; N2O EMISSIONS; GENERALIZED-MODEL; ECOSYSTEM MODEL; FLUXES; FERTILIZER; CH4; DENITRIFICATION; VARIABILITY; SIMULATION AB A unique high temporal frequency data set from an irrigated cotton-wheat rotation was used to test the agroecosystem model DayCent to simulate daily N2O emissions from subtropical vertisols under different irrigation intensities. DayCent was able to simulate the effect of different irrigation intensities on N2O fluxes and yield, although it tended to overestimate seasonal fluxes during the cotton season. DayCent accurately predicted soil moisture dynamics and the timing and magnitude of high fluxes associated with fertilizer additions and irrigation events. At the daily scale we found a good correlation of predicted vs. measured N2O fluxes (r(2) = 0.52), confirming that DayCent can be used to test agricultural practices for mitigating N2O emission from irrigated cropping systems. A 25-year scenario analysis indicated that N2O losses from irrigated cotton-wheat rotations on black vertisols in Australia can be substantially reduced by an optimized fertilizer and irrigation management system (i.e., frequent irrigation, avoidance of excessive fertilizer application), while sustaining maximum yield potentials. C1 [Scheer, Clemens; Rowlings, David W.; Grace, Peter R.] Queensland Univ Technol, Inst Future Environm, Brisbane, Qld 4000, Australia. [Del Grosso, Stephen J.] ARS, USDA, Nat Resources Res Ctr, Ft Collins, CO 80526 USA. [Del Grosso, Stephen J.; Parton, William J.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP Scheer, C (reprint author), Queensland Univ Technol, Inst Future Environm, Brisbane, Qld 4000, Australia. EM clemens.scheer@qut.edu.au OI Scheer, Clemens/0000-0001-5396-2076 FU Department of Agriculture, Fisheries and Forestry (DAFF); Grains Research and Development Corporation (GRDC) FX We thank the Department of Employment, Economic Development and Innovation (DEEDI) for providing the study site and the farm staff during the field measuring campaign. This research was undertaken as part of the National Agricultural Nitrous Oxide Research Program (NANORP) funded by the Department of Agriculture, Fisheries and Forestry (DAFF) and the Grains Research and Development Corporation (GRDC). We also thank three anonymous reviewers for valuable comments on an earlier version of the manuscript. NR 51 TC 9 Z9 10 U1 2 U2 54 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1051-0761 EI 1939-5582 J9 ECOL APPL JI Ecol. Appl. PD APR PY 2014 VL 24 IS 3 BP 528 EP 538 DI 10.1890/13-0570.1 PG 11 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AD4TA UT WOS:000333242300009 PM 24834738 ER PT J AU Resasco, J Porter, SD Sanders, NJ Levey, DJ AF Resasco, Julian Porter, Sanford D. Sanders, Nathan J. Levey, Douglas J. TI Assessing the effects of sodium on fire ant foraging in the field and colony growth in the laboratory SO ECOLOGICAL ENTOMOLOGY LA English DT Article C1 [Resasco, Julian] Univ Florida, Dept Biol, Gainesville, FL USA. [Porter, Sanford D.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. [Sanders, Nathan J.] Univ Tennessee, Dept Ecol & Evolutionary Biol, Knoxville, TN USA. [Levey, Douglas J.] Natl Sci Fdn, Arlington, VA 22230 USA. RP Resasco, J (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, UCB 334, Boulder, CO 80309 USA. EM jresasco@colorado.edu RI Sanders, Nathan/A-6945-2009; publist, CMEC/C-3010-2012; publicationpage, cmec/B-4405-2017 OI Sanders, Nathan/0000-0001-6220-6731; FU National Science Foundation [DGE-0802270] FX We thank Darrell Hall (USDA-ARS, Gainesville, FL) for his assistance with rearing colonies and M. Kaspari for conversations that improved this study. J.R. was supported by a National Science Foundation Graduate Research Fellowship under grant no. DGE-0802270. NR 0 TC 0 Z9 0 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0307-6946 EI 1365-2311 J9 ECOL ENTOMOL JI Ecol. Entomol. PD APR PY 2014 VL 39 IS 2 PG 5 WC Entomology SC Entomology GA AC4CM UT WOS:000332468200004 ER PT J AU Olden, JD Konrad, CP Melis, TS Kennard, MJ Freeman, MC Mims, MC Bray, EN Gido, KB Hemphill, NP Lytle, DA McMullen, LE Pyron, M Robinson, CT Schmidt, JC Williams, JG AF Olden, Julian D. Konrad, Christopher P. Melis, Theodore S. Kennard, Mark J. Freeman, Mary C. Mims, Meryl C. Bray, Erin N. Gido, Keith B. Hemphill, Nina P. Lytle, David A. McMullen, Laura E. Pyron, Mark Robinson, Christopher T. Schmidt, John C. Williams, John G. TI Are large-scale flow experiments informing the science and management of freshwater ecosystems? SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Article ID MODIFYING DAM OPERATIONS; MANAGING RIVER FLOWS; ADAPTIVE MANAGEMENT; ENVIRONMENTAL FLOWS; CLIMATE-CHANGE; EXPERIMENTAL FLOODS; DECISION-MAKING; GRAND-CANYON; RESPONSES; SYSTEMS AB Greater scientific knowledge, changing societal values, and legislative mandates have emphasized the importance of implementing large-scale flow experiments (FEs) downstream of dams. We provide the first global assessment of FEs to evaluate their success in advancing science and informing management decisions. Systematic review of 113 FEs across 20 countries revealed that clear articulation of experimental objectives, while not universally practiced, was crucial for achieving management outcomes and changing dam-operating policies. Furthermore, changes to dam operations were three times less likely when FEs were conducted primarily for scientific purposes. Despite the recognized importance of riverine flow regimes, four-fifths of FEs involved only discrete flow events. Over three-quarters of FEs documented both abiotic and biotic outcomes, but only one-third examined multiple taxonomic responses, thus limiting how FE results can inform holistic dam management. Future FEs will present new opportunities to advance scientifically credible water policies. C1 [Olden, Julian D.; Mims, Meryl C.; Williams, John G.] Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. [Konrad, Christopher P.] US Geol Survey, Washington Water Sci Ctr, Tacoma, WA USA. [Melis, Theodore S.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86001 USA. [Kennard, Mark J.] Griffith Univ, Northern Australia Hub & Australian Rivers Inst, Natl Environm Res Program, Nathan, Qld 4111, Australia. [Freeman, Mary C.] US Geol Survey, Patuxent Wildlife Res Ctr, Athens, GA USA. [Bray, Erin N.] Univ Calif Santa Barbara, Donald Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA. [Gido, Keith B.] Kansas State Univ, Div Biol, Manhattan, KS 66506 USA. [Hemphill, Nina P.] US Forest Serv, Sequoia Natl Forest, Porterville, CA USA. [Lytle, David A.] Oregon State Univ, Dept Zool, Corvallis, OR 97331 USA. [McMullen, Laura E.] ICF Int, Portland, OR USA. [Pyron, Mark] Ball State Univ, Dept Biol, Muncie, IN 47306 USA. [Robinson, Christopher T.] Swiss Fed Inst Aquat Sci & Technol, Dubendorf, Switzerland. [Schmidt, John C.] Utah State Univ, Dept Watershed Sci, Logan, UT 84322 USA. RP Olden, JD (reprint author), Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA. EM olden@uw.edu RI Kennard, Mark/C-3425-2008; Bray, Erin/P-3712-2016; Pyron, Mark/D-4572-2011; OI Kennard, Mark/0000-0003-4383-4999; Bray, Erin/0000-0001-7259-3210; Pyron, Mark/0000-0003-0451-7827; Olden, Julian/0000-0003-2143-1187; Konrad, Christopher/0000-0002-7354-547X; Mims, Meryl/0000-0003-0570-988X FU National Center for Ecological Analysis and Synthesis (NCEAS) [12374] FX We thank D Carlisle for his comments on the manuscript. This contribution is based on the workshop "Evaluating Responses of Freshwater Ecosystems to Experimental Water Management", funded by the National Center for Ecological Analysis and Synthesis (NCEAS Project 12374). NR 53 TC 27 Z9 28 U1 5 U2 51 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD APR PY 2014 VL 12 IS 3 BP 176 EP 185 DI 10.1890/130076 PG 10 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AD8XJ UT WOS:000333548700008 ER PT J AU Rojas, MG Morales-Ramos, JA AF Rojas, M. Guadalupe Morales-Ramos, Juan A. TI Juvenile Coloration as a Predictor of Health in Nezara viridula (Heteroptera: Pentatomidae) Rearing SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE southern green stink bug; survival; fecundity; rearing; nymphal coloration ID HEMIPTERA-PENTATOMIDAE; COTTON BOLLS; PATHOGENS; VECTORS AB Field collections of early nymphs of Nezara viridula (L.) revealed that normal change in coloration pattern occurring between the fourth and fifth stadia do not occur in some individuals producing abnormally-colored fifth instars. To determine if abnormal coloration is an indicator of poor health, field-collected fifth instars were grouped in 4 coloration pattern groups: (1) Green, normal coloration pattern; (2) faded, displaying similar patterns as "green" but with paler colors; (3) intermediate black-green; and (4) black, retaining fourth-instar coloration pattern. The fitness of nymphs within coloration pattern groups was measured by recording survival to adulthood and assessment of adult weight, fecundity, and egg viability. Adults originating from each nymphal coloration pattern were grouped to determine if the variability in nymphal coloration was hereditary. Survival from fifth instar to adult was significantly lower in nymphs displaying abnormal coloration patterns showing 21 and 12.5% survival in the black and faded groups, respectively, as compared with 76.2% in the normal green coloration group. Adults originating from nymphs of black coloration did not produce progeny, whereas adults originating from the faded coloration produced significantly less eggs with lower viability than adults originating from nymphs with normal coloration. However, adults originating from normally-colored nymphs produced progeny of abnormal coloration in similar frequency as adults originating from black and intermediate colored nymphs. Abnormal nymphal coloration can be used as an indicator of health and can aid in the selection of healthy individuals for culture. C1 [Rojas, M. Guadalupe; Morales-Ramos, Juan A.] USDA ARS, Natl Biol Control Lab, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. RP Rojas, MG (reprint author), USDA ARS, Natl Biol Control Lab, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. EM guadalupe.rojas@ars.usda.gov NR 10 TC 3 Z9 3 U1 1 U2 5 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2014 VL 49 IS 2 BP 166 EP 175 PG 10 WC Entomology SC Entomology GA AI0QK UT WOS:000336553500007 ER PT J AU Cooper, WR Horton, DR AF Cooper, W. R. Horton, D. R. TI Gender- and Species-Specific Characteristics of Bacteriomes from Three Psyllid Species (Hemiptera: Psylloidea) SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE potato psyllid; pear psylla; mycetome; mycetocytes C1 [Cooper, W. R.; Horton, D. R.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. RP Cooper, WR (reprint author), USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. EM Rodney.Cooper@ars.usda.gov RI Cooper, William/D-3205-2017 NR 0 TC 2 Z9 2 U1 0 U2 3 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2014 VL 49 IS 2 BP 190 EP 194 PG 5 WC Entomology SC Entomology GA AI0QK UT WOS:000336553500009 ER PT J AU Meeker, JR Johnson, CW Steiner, CA Bruce, WW Gandhi, KJK AF Meeker, James R. Johnson, C. Wood Steiner, Chris A. Bruce, William W. Gandhi, Kama J. K. TI Timing of Adult Emergence and Flight Period of Rhyssa howdenorum (Hymenoptera: lchneumonidae), a Parasitoid of Sirex nigricornis (Hymenoptera: Siricidae), in Central Louisiana SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Rhyssa howdenorum; parasitoid; Sirex nigricornis; woodwasp; Pinus taeda; loblolly pine C1 [Meeker, James R.; Johnson, C. Wood; Steiner, Chris A.; Bruce, William W.; Gandhi, Kama J. K.] US Forest Serv, Alexandria Field Off, USDA, Pineville, LA 71360 USA. RP Meeker, JR (reprint author), US Forest Serv, Alexandria Field Off, USDA, 2500 Shreveport Highway, Pineville, LA 71360 USA. EM jrmeeker@fs.fed.us FU U.S.D.A. Forest Service, Forest Health Protection FX The authors thank Alex Mangini of the Alexandria Field Office, Forest Health Protection, U.S.D.A. Forest Service, for various forms of field assistance throughout this project, and critical review of an earlier version of this manuscript which greatly improved such. We also thank Bobby Joe Ray and Anthony Page of the Catahoula Ranger District, Kisatchie National Forest, for graciously providing us the necessary support and space to conduct these investigations at the Catahoula Work Center. Funding was provided by the U.S.D.A. Forest Service, Forest Health Protection, 2009 Request for Research and Technology Development Proposals to Improve the Management and Detection of Sirex noctilio. Voucher specimens are deposited in the Louisiana State Arthropod Museum, Baton Rouge, LA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2014 VL 49 IS 2 BP 206 EP 210 PG 5 WC Entomology SC Entomology GA AI0QK UT WOS:000336553500012 ER PT J AU Suh, CPC Westbrook, JK AF Suh, C. P. -C. Westbrook, J. K. TI Failure of Pheromone Traps in Detecting Incipient Populations of Boll Weevils (Coleoptera: Curculiondae): Investigation of Two Potential Contributing Factors SO JOURNAL OF ENTOMOLOGICAL SCIENCE LA English DT Article DE Boll weevil; Anthonomus grandis; pheromone trap; grandlure C1 [Suh, C. P. -C.; Westbrook, J. K.] ARS, USDA, Areawide Pest Management Res Unit, College Stn, TX 77845 USA. RP Suh, CPC (reprint author), ARS, USDA, Areawide Pest Management Res Unit, 2771 F&B Rd, College Stn, TX 77845 USA. EM charles.suh@ars.usda.gov NR 0 TC 0 Z9 0 U1 3 U2 4 PU GEORGIA ENTOMOLOGICAL SOC INC PI TIFTON PA PO BOX 748 DEPT ENTOMOLOGY COASTAL PLAIN EXPT STATION, TIFTON, GA 31793-0748 USA SN 0749-8004 J9 J ENTOMOL SCI JI J. Entomol. Sci. PD APR PY 2014 VL 49 IS 2 BP 211 EP 214 PG 4 WC Entomology SC Entomology GA AI0QK UT WOS:000336553500013 ER PT J AU Wren, DG Kuhnle, RA Langendoen, EJ Rigby, JR AF Wren, Daniel G. Kuhnle, Roger A. Langendoen, Eddy J. Rigby, J. R. TI Turbulent Flow and Sand Transport over a Cobble Bed in a Laboratory Flume SO JOURNAL OF HYDRAULIC ENGINEERING LA English DT Article DE Sediment; Turbulent flow; Gravel; Flumes; Sediment; Turbulence; Cobbles; Gravel ID DOUBLE-AVERAGING CONCEPT; OPEN-CHANNEL; SEDIMENT TRANSPORT; OVERLAND FLOWS; GRAVEL-BEDS; RESISTANCE; VELOCITY; SLOPES; MOTION; RIVER AB Improving the prediction of sand transport downstream of dams requires characterization of the interaction between turbulent flow and near-surface interstitial sands. The advanced age and impending decommissioning of many dams have brought increased attention to the fate of sediments stored in reservoirs. Sands can be reintroduced to coarse substrates that have available pore space resulting from periods of sediment-starved flow. The roughness and porosity of the coarse substrate are both affected by sand elevation relative to the coarse substrate; therefore, the turbulence characteristics and sediment transport over and through these beds are significantly altered after sediment is reintroduced. Past work by the writers on flow over sand-filled gravel beds revealed that the fine-sediment level controls the volume of material available for transport and the area of sediment exposed to the flow. The present work expands on the gravel-bed experiments by conducting similar measurements of turbulent flow and sand transport over a bed of cobbles with a median diameter of approximately 150mm. This change in scale expands the generality of the previous experiments and broadens the range of sand transport and turbulence measurements. It was found that the same relationship between bed shear stress and sand elevation was valid for both gravel and cobble systems. Reductions in bed shear stress, relative to the clear-water case, were observed as the sand elevation was increased, although the highest sand elevation did not yield the lowest shear stress. Quadrant analysis showed that, for stronger turbulent events, there was an increase of sweeps and a decrease in bursts as the sand level was raised. This effect was observed for a region with a height of approximately 1.4 times the thickness of the roughness layer. C1 [Wren, Daniel G.; Kuhnle, Roger A.; Langendoen, Eddy J.; Rigby, J. R.] ARS, USDA, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Wren, DG (reprint author), ARS, USDA, Natl Sedimentat Lab, 598 McElroy Dr, Oxford, MS 38655 USA. EM daniel.wren@ars.usda.gov; roger.kuhnle@ars.usda.gov; eddy.langendoen@ars.usda.gov; jr.rigby@ars.usda.gov OI Rigby, James/0000-0002-5611-6307; Langendoen, Eddy/0000-0002-2215-4989 NR 42 TC 3 Z9 3 U1 0 U2 23 PU ASCE-AMER SOC CIVIL ENGINEERS PI RESTON PA 1801 ALEXANDER BELL DR, RESTON, VA 20191-4400 USA SN 0733-9429 EI 1943-7900 J9 J HYDRAUL ENG JI J. Hydraul. Eng.-ASCE PD APR 1 PY 2014 VL 140 IS 4 AR 04014001 DI 10.1061/(ASCE)HY.1943-7900.0000838 PG 12 WC Engineering, Civil; Engineering, Mechanical; Water Resources SC Engineering; Water Resources GA AC7XJ UT WOS:000332747300009 ER PT J AU Morales, JK Saleem, SJ Martin, RK Saunders, BL Barnstein, BO Faber, TW Pullen, NA Kolawole, EM Brooks, KB Norton, SK Sturgill, J Graham, L Bear, HD Urban, JF Lantz, CS Conrad, DH Ryan, JJ AF Morales, Johanna K. Saleem, Sheinei J. Martin, Rebecca K. Saunders, Bryan L. Barnstein, Brian O. Faber, Travis W. Pullen, Nicholas A. Kolawole, Elizabeth Motunrayo Brooks, Keith B. Norton, Sarah K. Sturgill, Jamie Graham, Laura Bear, Harry D. Urban, Joseph F., Jr. Lantz, Chris S. Conrad, Daniel H. Ryan, John J. TI Myeloid-derived suppressor cells enhance IgE-mediated mast cell responses SO JOURNAL OF LEUKOCYTE BIOLOGY LA English DT Article DE Nippostrongylus; Trichinella; asthma; allergy; inflammation ID PARASITE NIPPOSTRONGYLUS-BRASILIENSIS; TUMOR-BEARING MICE; T-CELLS; TRICHINELLA-SPIRALIS; COMBINATION THERAPY; INFLAMMATION; GEMCITABINE; MECHANISMS; IMMUNITY; CANCER AB MDSC enhance mast cell inflammatory responses that can be altered by an existing chemotherapeutic gemcitabine. Mast cells and MDSCs are increased by parasitic infection and tumor growth. We previously demonstrated that enhanced MDSC development in ADAM10 transgenic mice yielded resistance to Nb infection and that coculturing MDSCs and mast cells enhanced cytokine production. In the current work, we show that MDSC-mast cell coculture selectively enhances IgE-mediated cytokine secretion among mast cells, without increasing MDSC cytokine production. This effect was independent of cell contact and elicited by Ly6C(+) and Ly6C/G+ MDSC subsets. These interactions were functionally important. MDSC depletion with the FDA-approved drug gemcitabine exacerbated Nb or Trichinella spiralis infection and reduced mast cell-dependent AHR and lung inflammation. Adoptive transfer of MDSC worsened AHR in WT but not mast cell-deficient Wsh/Wsh mice. These data support the hypothesis that MDSCs enhance mast cell inflammatory responses and demonstrate that this interaction can be altered by an existing chemotherapeutic. C1 [Morales, Johanna K.; Barnstein, Brian O.; Faber, Travis W.; Pullen, Nicholas A.; Kolawole, Elizabeth Motunrayo; Ryan, John J.] Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. [Saleem, Sheinei J.; Martin, Rebecca K.; Brooks, Keith B.; Norton, Sarah K.; Sturgill, Jamie; Conrad, Daniel H.] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23284 USA. [Saunders, Bryan L.; Graham, Laura; Bear, Harry D.] Virginia Commonwealth Univ, Div Surg Oncol, Dept Surg, Massey Canc Ctr, Richmond, VA 23284 USA. [Saunders, Bryan L.; Lantz, Chris S.] James Madison Univ, Dept Biol, Harrisonburg, VA 22807 USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. [Urban, Joseph F., Jr.] ARS, USDA, Beltsville Human Nutr Res Ctr, Diet Genom & Immunol Lab, Beltsville, MD USA. RP Ryan, JJ (reprint author), Virginia Commonwealth Univ, Dept Biol, Richmond, VA 23284 USA. EM jjryan@vcu.edu OI Urban, Joseph/0000-0002-1590-8869 FU U.S. National Institutes of Health [1R01AI59638, U19AI077435] FX Support for this work was provided by grants from the U.S. National Institutes of Health (1R01AI59638 to J.J.R. and U19AI077435 to J.J.R. and D.H.C.). NR 36 TC 7 Z9 7 U1 1 U2 4 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0741-5400 EI 1938-3673 J9 J LEUKOCYTE BIOL JI J. Leukoc. Biol. PD APR PY 2014 VL 95 IS 4 BP 643 EP 650 DI 10.1189/jlb.0913510 PG 8 WC Cell Biology; Hematology; Immunology SC Cell Biology; Hematology; Immunology GA AG3UW UT WOS:000335346300009 PM 24338630 ER PT J AU Chimezie, C Ewing, AC Quadri, SS Cole, RB Boue, SM Omari, CF Bratton, M Glotser, E Skripnikova, E Townley, I Stratford, RE AF Chimezie, Chukwuemezie Ewing, Adina C. Quadri, Syeda S. Cole, Richard B. Boue, Stephen M. Omari, Christopher F. Bratton, Melyssa Glotser, Elena Skripnikova, Elena Townley, Ian Stratford, Robert E., Jr. TI Glyceollin Transport, Metabolism, and Effects on P-Glycoprotein Function in Caco-2 Cells SO JOURNAL OF MEDICINAL FOOD LA English DT Article DE diet; flavonoids; genistein; glyceollin; induction of Pgp; inhibition of Pgp; isoflavone; phytoestrogen ID INTESTINAL EPITHELIAL-CELLS; INFLAMMATORY-BOWEL-DISEASE; MULTIDRUG-RESISTANCE-1 GENE; MULTIDRUG-RESISTANCE; DIETARY FLAVONOIDS; DRUG-INTERACTIONS; IN-VITRO; GENISTEIN; DISPOSITION; ABSORPTION AB Glyceollins are phytoalexins produced in soybeans from their isoflavone precursor daidzein. Their impressive anticancer and glucose normalization effects in rodents have generated interest in their therapeutic potential. The aim of the present studies was to begin to understand glyceollin intestinal transport and metabolism, and their potential effects on P-glycoprotein (Pgp) in Caco-2 cells. At 10 and 25 mu M, glyceollin permeability was 2.4 +/- 0.16 x 10(-4) cm/sec and 2.1 +/- 0.15 x 10(-4) cm/sec, respectively, in the absorptive direction. Basolateral to apical permeability at 25 mu M was 1.6 +/- 0.10 x 10(-4) cm/sec. Results suggest high absorption potential of glyceollin by a passive-diffusion-dominated mechanism. A sulfate conjugate at the phenolic hydroxyl position was observed following exposure to Caco-2 cells. In contrast to verapamil inhibition of the net secretory permeability of rhodamine 123 (R123) and its enhancement of calcein AM uptake into Caco-2 cells, neither glyceollin nor genistein inhibited Pgp (MDR1; ABCB1) up to 300 mu M. There was no significant change in MDR1 mRNA expression, Pgp protein expression, or R123 transport in cells exposed to glyceollin or genistein for 24 h up to 100 mu M. Collectively, these results suggest that glyceollin has the potential to be well absorbed, but that, similar to the isoflavone genistein, its absorption may be reduced substantially by intestinal metabolism; further, they indicate that glyceollin does not appear to alter Pgp function in Caco-2 cells. C1 [Chimezie, Chukwuemezie; Ewing, Adina C.; Omari, Christopher F.; Bratton, Melyssa; Glotser, Elena; Skripnikova, Elena; Townley, Ian; Stratford, Robert E., Jr.] Xavier Univ Louisiana, Coll Pharm, Dept Basic Pharmaceut Sci, New Orleans, LA 70125 USA. [Quadri, Syeda S.; Cole, Richard B.] Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. [Cole, Richard B.] Univ Paris 06, Lab Organ & Biol Struct Chem, Paris, France. [Boue, Stephen M.] USDA, So Reg Res Ctr, New Orleans, LA 70179 USA. RP Stratford, RE (reprint author), Xavier Univ Louisiana, Coll Pharm, Dept Basic Pharmaceut Sci, 1 Drexel Dr, New Orleans, LA 70125 USA. EM rstratfo@xula.edu FU Louisiana Cancer Research Consortium Seed Grant [OSP-7011-003]; National Institutes on Minority Health and Health Disparities (NIMHD), National Institutes of Health (NIH), and Department of Health and Human Services (DHHS) [8G12MD007595-04] FX This work was funded by a Louisiana Cancer Research Consortium Seed Grant (OSP-7011-003) and in part by Grant Number 8G12MD007595-04 from the National Institutes on Minority Health and Health Disparities (NIMHD), National Institutes of Health (NIH), and Department of Health and Human Services (DHHS). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIMHD or NIH. NR 47 TC 2 Z9 2 U1 2 U2 21 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1096-620X EI 1557-7600 J9 J MED FOOD JI J. Med. Food PD APR PY 2014 VL 17 IS 4 BP 462 EP 471 DI 10.1089/jmf.2013.0115 PG 10 WC Chemistry, Medicinal; Food Science & Technology; Nutrition & Dietetics SC Pharmacology & Pharmacy; Food Science & Technology; Nutrition & Dietetics GA AH6CB UT WOS:000336217200008 PM 24476214 ER PT J AU Pedersen, K Quance, CR Robbe-Austerman, S Piaggio, AJ Bevins, SN Goldstein, SM Gaston, WD DeLiberto, TJ AF Pedersen, Kerri Quance, Christine R. Robbe-Austerman, Suelee Piaggio, Antoinette J. Bevins, Sarah N. Goldstein, Samuel M. Gaston, Wesson D. DeLiberto, Thomas J. TI IDENTIFICATION OF BRUCELLA SUIS FROM FERAL SWINE IN SELECTED STATES IN THE USA SO JOURNAL OF WILDLIFE DISEASES LA English DT Article DE Brucella suis; brucellosis; culture; feral swine; genotype; isolate; Sus scrofa; VNTR ID DISEASE TRANSMISSION; UNITED-STATES; CATTLE; DIFFERENTIATION; DIAGNOSIS; SEROLOGY; CONTACT; BISON; ASSAY; PCR AB Serologic tests currently available for brucellosis diagnosis detect antibodies to Brucella but do not distinguish between species of Brucella. Although Brucella suis is known to circulate within various feral swine (Sus scrofa) populations, our objective was to determine the primary species of Brucella circulating in feral swine populations in areas of the US with high brucellosis prevalence. We cultured lymph nodes from 183 feral swine. We identified 22 isolates from 21 animals, and all isolates were genotyped as B. suis. Most isolates were B. suis biovar 1, with the exception of two genetically distinct isolates from one feral swine in Hawaii, which were identified as B. suis biovar 3. Serum from each feral swine was also tested by the fluorescence polarization assay when possible, but only 52% (95% CL=29.8-74.3) of culture-positive animals were antibody positive. Our results indicate that brucellosis infections in feral swine within the US are typically caused by B. suis. However, improved serologic tests are needed to more accurately determine exposure to Brucella spp. and to monitor disease trends in feral swine populations. C1 [Pedersen, Kerri; Piaggio, Antoinette J.; DeLiberto, Thomas J.] Wildlife Serv, Natl Wildlife Res Ctr, US Anim & Plant Hlth Inspect Serv, USDA, Ft Collins, CO 80521 USA. [Quance, Christine R.; Robbe-Austerman, Suelee] Vet Serv, Natl Vet Serv Labs, US Anim & Plant Hlth Inspect Serv, USDA, Ames, IA 50010 USA. [Bevins, Sarah N.] Colorado State Univ, Dept Biomed Sci, Ft Collins, CO 80523 USA. [Goldstein, Samuel M.] Wildlife Serv, US Anim & Plant Hlth Inspect Serv, USDA, Honolulu, HI 96819 USA. [Gaston, Wesson D.] Wildlife Serv, US Anim & Plant Hlth Inspect Serv, USDA, Montgomery, AL 36107 USA. RP Pedersen, K (reprint author), Wildlife Serv, Natl Wildlife Res Ctr, US Anim & Plant Hlth Inspect Serv, USDA, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Kerri.Pedersen@aphis.usda.gov NR 35 TC 2 Z9 2 U1 1 U2 8 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD APR PY 2014 VL 50 IS 2 BP 171 EP 179 DI 10.7589/2013-09-235 PG 9 WC Veterinary Sciences SC Veterinary Sciences GA AH6JT UT WOS:000336237900002 PM 24484477 ER PT J AU Welsh, T Burek-Huntington, K Savage, K Rosenthal, B Dubey, JP AF Welsh, Trista Burek-Huntington, Kathy Savage, Kate Rosenthal, Benjamin Dubey, J. P. TI Sarcocystis canis Associated Hepatitis in a Steller Sea Lion (Eumetopias jubatus) from Alaska SO JOURNAL OF WILDLIFE DISEASES LA English DT Article ID TOXOPLASMA-GONDII; NEOSPORA-CANINUM; INFECTIONS; PARASITES; NEURONA AB Sarcocystis canis infection was associated with hepatitis in a Steller sea lion (Eumetopias jubatus). Intrahepatocellular protozoal schizonts were among areas of necrosis and inflammation. The parasite was genetically identical to S. canis and is the first report in a Steller sea lion, indicating another intermediate host species for S. canis. C1 [Welsh, Trista] Univ Georgia, Coll Vet Med, Athens, GA 30605 USA. [Burek-Huntington, Kathy] Alaska Vet Pathol Serv, Eagle River, AK 99577 USA. [Savage, Kate] Natl Marine Fisheries Serv, Protected Resources Div, Juneau, AK 99802 USA. [Rosenthal, Benjamin; Dubey, J. P.] ARS, USDA, Beltsville Agr Res Ctr, Anim Parasit Dis Lab,BARC East, Beltsville, MD 20705 USA. RP Welsh, T (reprint author), Flyinvet, 10174 Airpark Loop, Givens Hotsprings, ID 83641 USA. EM trista.welsh@gmail.com OI Rosenthal, Benjamin/0000-0002-0224-3773 NR 10 TC 0 Z9 0 U1 2 U2 12 PU WILDLIFE DISEASE ASSOC, INC PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0090-3558 EI 1943-3700 J9 J WILDLIFE DIS JI J. Wildl. Dis. PD APR PY 2014 VL 50 IS 2 BP 405 EP 408 DI 10.7589/2013-03-079 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA AH6JT UT WOS:000336237900036 PM 24484486 ER PT J AU Amelon, SK Thompson, FR Millspaugh, JJ AF Amelon, Sybill K. Thompson, Frank R., III Millspaugh, Joshua J. TI Resource Utilization by Foraging Eastern Red Bats (Lasiurus borealis) in the Ozark Region of Missouri SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE eastern red bat; foraging; Lasiurus borealis; resource utilization distribution ID WHITE-NOSE SYNDROME; FEMALE HOARY BATS; HABITAT USE; INSECTIVOROUS BATS; NORTH-AMERICA; UTILIZATION DISTRIBUTIONS; ECHOLOCATING BATS; ROOST SELECTION; SOUTH-CAROLINA; PREY SELECTION AB Resource selection by animals influences ecological processes such as dispersal, reproduction, foraging, and migration. Little information exists regarding foraging resource selection by bats during the maternity season. We evaluated support for effects of landcover type, landform, and landscape pattern on resource selection by individual foraging female eastern red bats (Lasiurus borealis) during the maternity period and compared resource utilization for all individuals pooled (population level), individuals grouped by geographic location, and individuals grouped by stage of lactation (early, mid, and late). We used a resource utilization function (RUF) to relate landcover and landscape attributes to the utilization distributions of individual bats estimated by the fixed-kernel method. We radio-tracked 64 lactating red bats and estimated utilization distributions for 52 individuals. Mean home range size ranged from 1,041 to 1,588 ha from late to mid lactation. The global RUF model was significantly better than the null RUF model for 36 (70%) individuals and the magnitude and direction of coefficients varied among individuals. Resource utilization at the population level was, on average, positively related to ridges and upland drainage landforms, water landcover, and road density; and negatively related to urban and nonforest landcover and distance to edge. Resource use differed between geographic areas by canopy cover, water landcover, and road density. Canopy closure was positively related to RUF in both areas but was greater in the south area. Percent water negatively related to RUF in the north area and positively related in the south area. Road density had a positive relationship in the north and negative relationship in the south with RUF. We did not find a difference in mean RUF coefficients among lactation groups except for canopy cover. On average, canopy cover had a positive effect on use by bats during early and mid-lactation but a negative effect during late lactation. We suggest that management for red bats consider landscape components and provide a range of composition and structural diversity to enhance foraging use by red bats. In highly forested landscapes, gaps or openings may provide forest edges that are important for foraging and commuting. Published 2014. This article is a U. S. Government work and is in the public domain in the USA. C1 [Amelon, Sybill K.; Thompson, Frank R., III] US Forest Serv, No Res Stn, USDA, Columbia, MO 65211 USA. [Millspaugh, Joshua J.] Univ Missouri, Columbia, MO 65211 USA. RP Amelon, SK (reprint author), US Forest Serv, No Res Stn, USDA, 202 Nat Resource Bldg, Columbia, MO 65211 USA. EM samelon@fs.fed.us OI Amelon, Sybill/0000-0003-3940-8153 FU United States Department of Agriculture Forest Service Northern Research Station; Mark Twain National Forest FX We thank the United States Department of Agriculture Forest Service Northern Research Station and the Mark Twain National Forest for funding this project. We especially thank W. Dijak, K. Heun, L. Frasher, J. McMeachim, J. Amelon, E. Hamilton, K. Thompson, J. Eberly, and P. and L. Smeltzer for logistic and field support. NR 88 TC 2 Z9 2 U1 0 U2 54 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD APR PY 2014 VL 78 IS 3 BP 483 EP 493 DI 10.1002/jwmg.685 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AH3MI UT WOS:000336027400012 ER PT J AU Deluca, WV King, DI AF Deluca, William V. King, David I. TI Influence of Hiking Trails on Montane Birds SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE abundance; blackpoll warbler; Dail-Madsen model; detection probability; montane forests; recruitment; White Mountains ID NORTHEASTERN UNITED-STATES; NEW-HAMPSHIRE; BICKNELLS THRUSH; HUMAN-INTRUSION; HIERARCHICAL-MODELS; RECREATIONAL TRAILS; AVIAN POPULATION; HABITAT QUALITY; CLIMATE-CHANGE; SITE FIDELITY AB Montane forests contribute significantly to regional biodiversity. Long-term monitoring data, often located along hiking trails, suggests that several indicator species of this ecosystem have declined in recent decades. Declining montane bird populations have been attributed to anthropogenic stressors such as climate change and atmospheric deposition. Several studies from montane systems have also documented decreased estimates of abundance and reproductive success as well as altered singing rates near hiking trails. Therefore, recreational hiking may be contributing to montane bird population declines and potentially biased population estimates because of trail avoidance or altered detection probabilities near trails. We studied the effect of hiking trails on the abundance, seasonal movements, and detection probabilities of montane birds in the White Mountains, New Hampshire in 2006 and 2007. We used hierarchical, generalized N-mixture models that account for imperfect detection probabilities to examine the effects of recreational hiking on bird communities. We also examined the potential effects of hiking on the reproductive success of a boreal forest songbird, blackpoll warbler (Setophaga striata). We found little evidence to suggest hiking trails influence abundance, detection probabilities, or within and among seasonal movements of montane forest birds. We also found no evidence to suggest daily nest survival of blackpoll warbler nests vary with distance from trail. Our study suggests that recent increases in hiking traffic are unlikely to have caused declines in montane birds in this region. Furthermore, our results provide evidence that trail-based monitoring programs can provide accurate and efficient estimates of abundance for some montane forest bird species in the White Mountains of New Hampshire. (C) 2014 The Wildlife Society. C1 [Deluca, William V.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [King, David I.] Univ Massachusetts, US Forest Serv, No Res Stn, USDA, Amherst, MA 01003 USA. RP Deluca, WV (reprint author), Univ Massachusetts, Dept Environm Conservat, 204 Holdsworth Hall, Amherst, MA 01003 USA. EM wdeluca@eco.umass.edu FU White Mountain National Forest; American Bird Conservancy FX We thank the White Mountain National Forest and the American Bird Conservancy for financial support. We are grateful to J. Woodcock, C. Shepard, J. Glagowski, N. Paprocki, and N. Marcy for their meticulous data collection. C. Costello and the Bartlett Experimental Forest were gracious hosts. We also thank L. Prout for her logistical support. NR 42 TC 2 Z9 2 U1 5 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD APR PY 2014 VL 78 IS 3 BP 494 EP 502 DI 10.1002/jwmg.675 PG 9 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AH3MI UT WOS:000336027400013 ER PT J AU Kweon, HJ Choi, DG Lee, J Kang, IK AF Kweon, Hun-Joong Choi, Dong Geun Lee, Jinwook Kang, In-Kyu TI Fruit Quality Characteristics of 'Fuji' Apple Fruits in Response to Air Storage after CA Storage SO KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY LA Korean DT Article DE ethylene; flesh browning; flesh firmness; titratable acidity ID CONTROLLED-ATMOSPHERE; CO2 INJURY; DISORDERS; TEMPERATURE; WATERCORE; MATURITY; OXYGEN; DELAYS AB This study aimed to evaluate the fruit quality characteristics and incidence of flesh browning in response to air storage at 0 +/- 1 degrees C after controlled atmosphere storage (CA condition: O-2 2.5 +/- 0.5%, CO2 1.5 +/- 0.5%) at 0 +/- 1 degrees C in 'Fuji' apples (Malus domestica Borkh.). The storage system was performed as followed: air storage for one month, CA storage 4 months + air storage 3 months (CA 4M + Air 3M), CA storage 5 months + air storage 2 months (CA 5M + Air 2M) and CA storage 6 months + air storage 1months (CA 6M + Air 1M), while the control fruits were stored at CA storage for 8 months right after harvest. The incidence of flesh browning ranged from 17.1% to 30.2% during CA storage but not detected under the treatments of CA 4M + Air 3M and CA 5M + Air 2M. The respiration rate was not affected by storage treatments for 6M while the respiration rate was lower in the treatments of CA 4M + Air 3M and CA 5M + Air 2M than the other storage treatments after 7 months. Ethylene production and internal ethylene concentration were lowest in rapid CA storage and increased with a decreasing CA storage duration. Therefore, the results indicate that CA 5M + Air 2M storage treatment should be recommended to maintain the fruit quality and reduce the risk development of flesh browning rather than typical CA storage in 'Fuji' apples. C1 [Kweon, Hun-Joong] Natl Inst Hort & Herbal Sci, Apple Res Stn, Gunwi 716810, South Korea. [Choi, Dong Geun] Chonbuk Natl Univ, Dept Hort Sci, Jeonju 570752, South Korea. [Lee, Jinwook] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Kang, In-Kyu] Kyungpook Natl Univ, Dept Hort Sci, Taegu 702701, South Korea. RP Kang, IK (reprint author), Kyungpook Natl Univ, Dept Hort Sci, Taegu 702701, South Korea. EM kangik@knu.ac.kr NR 21 TC 0 Z9 0 U1 2 U2 11 PU KOREAN SOC HORTICULTURAL SCIENCE PI SUWON PA C/O NATL HORTICULTURAL RES INST, IMOK-DONG 475, JANGAN-GU, SUWON, 440-706, SOUTH KOREA SN 1226-8763 J9 KOREAN J HORTIC SCI JI Korean J. Hortic. Sci. Technol. PD APR PY 2014 VL 32 IS 2 BP 178 EP 183 DI 10.7235/hort.2014.13110 PG 6 WC Horticulture SC Agriculture GA AH8VF UT WOS:000336415600007 ER PT J AU Ali, A Yang, EM Bang, SW Chung, SM Staub, JE AF Ali, Asjad Yang, Eun Mi Bang, Sun Woong Chung, Sang-Min Staub, Jack E. TI Assessment of Chilling Injury and Molecular Marker Analysis in Cucumber Cultivars (Cucumis sativus L.) SO KOREAN JOURNAL OF HORTICULTURAL SCIENCE & TECHNOLOGY LA English DT Article DE abiotic stress; chloroplast genotypes; DNA marker; low temperature; sdCAPS ID LOW-TEMPERATURE; PHOTOSYSTEM-I; ASSISTED SELECTION; GENETIC-VARIATION; PLANTS; TOLERANCE; LIGHT; PHOTOINHIBITION; ACCLIMATION; GROWTH AB The responses to chilling temperature of 12 Korean cucumber varieties was compared to those of two U.S.A. (previously determined cold tolerant NC76 and 'Chipper'), and Chinese and Japanese germplasms. Seedlings of each entry were exposed to 4 degrees C (Experiment 1) and 1 degrees C (Experiments 2 and 3) at the first-true leaf stage for eight and nine hours, respectively, under 80% relative humidity (RH) and 149 mu moles.m(-2).s(-1) photosynthetic photon flux (PPF). The chilling response [damage rating (DR)] of each accession was based on visual ratings (1 to 5) after treatment, where 1 = no damage, 2 = slight, 3 = moderate, 4 = advanced, and 5 = severe damage. Predictably the cumulative average DR of chilling tolerant line NC76 and 'Chipper' after chilling was 1 and 1.1, respectively. Korean 'Nacdongchungjang' was most sensitive to chilling temperatures [DR = 2.3] when compared to the other entries examined. The sensitivity to chilling of 'Nacdongchungjang' was followed by Chinese 'Dongguan' [DR = 1.7]. In contrast, 'Saeronchungjang' (DR = 1) and 'Janghyungnachap' (DR = 1) were the most chilling tolerant of the Korean accessions examined and equivalent to the response of line NC76 and 'Chipper'. Nevertheless, chloroplast type genotyping of these accessions with known chilling-linked sdCAPS genomic markers revealed genotypic differences between chilling tolerant lines (NC76 and 'Chipper') and all Korean lines examined. C1 [Ali, Asjad; Yang, Eun Mi; Bang, Sun Woong; Chung, Sang-Min] Dongguk Univ Seoul, Dept Life Sci, Seoul 100715, South Korea. [Staub, Jack E.] ARS, USDA, Forage & Range Res Lab, Logan, UT 84322 USA. RP Chung, SM (reprint author), Dongguk Univ Seoul, Dept Life Sci, Seoul 100715, South Korea. EM smchung@dongguk.edu FU Dongguk University FX This work was supported by the Dongguk University Research Fund of 2012. NR 28 TC 0 Z9 0 U1 3 U2 9 PU KOREAN SOC HORTICULTURAL SCIENCE PI SUWON PA C/O NATL HORTICULTURAL RES INST, IMOK-DONG 475, JANGAN-GU, SUWON, 440-706, SOUTH KOREA SN 1226-8763 J9 KOREAN J HORTIC SCI JI Korean J. Hortic. Sci. Technol. PD APR PY 2014 VL 32 IS 2 BP 227 EP 234 DI 10.7235/hort.2014.13138 PG 8 WC Horticulture SC Agriculture GA AH8VF UT WOS:000336415600013 ER PT J AU Barron-Gafford, GA Cable, JM Bentley, LP Scott, RL Huxman, TE Jenerette, GD Ogle, K AF Barron-Gafford, Greg A. Cable, Jessica M. Bentley, Lisa Patrick Scott, Russell L. Huxman, Travis E. Jenerette, G. Darrel Ogle, Kiona TI Quantifying the timescales over which exogenous and endogenous conditions affect soil respiration SO NEW PHYTOLOGIST LA English DT Article DE antecedent conditions; Bayesian modeling; lag effects; mesquite; photosynthesis; Prosopis velutina; soil respiration; woody plant encroachment ID WOODY-PLANT ENCROACHMENT; EXPLORATORY DATA-ANALYSIS; ECOSYSTEM RESPIRATION; PRECIPITATION PULSES; WATER RELATIONS; CLIMATE-CHANGE; CARBON FLUXES; TEMPERATURE; PHOTOSYNTHESIS; EXCHANGE AB Understanding how exogenous and endogenous factors and above-ground-below-ground linkages modulate carbon dynamics is difficult because of the influences of antecedent conditions. For example, there are variable lags between above-ground assimilation and below-ground efflux, and the duration of antecedent periods are often arbitrarily assigned. Nonetheless, developing models linking above- and below-ground processes is crucial for estimating current and future carbon dynamics. We collected data on leaf-level photosynthesis (A(sat)) and soil respiration (R-soil) in different microhabitats (under shrubs vs under bunchgrasses) in the Sonoran Desert. We evaluated timescales over which endogenous and exogenous factors control R-soil by analyzing data in the context of a semimechanistic temperature-response model of R-soil that incorporated effects of antecedent exogenous (soil water) and endogenous (A(sat)) conditions. For both microhabitats, antecedent soil water and A(sat) significantly affected R-soil, but R-soil under shrubs was more sensitive to A(sat) than that under bunchgrasses. Photosynthetic rates 1 and 3d before the R-soil measurement were most important in determining current-day R-soil under bunchgrasses and shrubs, respectively, indicating a significant lag effect. Endogenous and exogenous controls are critical drivers of R-soil, but the relative importance and the timescale over which each factor affects R-soil depends on above-ground vegetation and ecosystem structure characteristics. C1 [Barron-Gafford, Greg A.] Univ Arizona, Sch Geog & Dev, Tucson, AZ 85721 USA. [Barron-Gafford, Greg A.] Univ Arizona, Tucson, AZ 85721 USA. [Cable, Jessica M.] Univ Alaska Fairbanks, Int Arctic Res Ctr, Fairbanks, AK 99775 USA. [Bentley, Lisa Patrick] Univ Oxford, Oxford Univ Ctr Environm, Environm Change Inst, Oxford OX1 3QY, England. [Scott, Russell L.] ARS, Southwest Watershed Res Ctr, USDA, Tucson, AZ 85719 USA. [Huxman, Travis E.] Univ Calif Irvine, Irvine, CA 92697 USA. [Huxman, Travis E.] Univ Calif Irvine, Ctr Environm Biol, Irvine, CA 92697 USA. [Jenerette, G. Darrel] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Ogle, Kiona] Arizona State Univ, Sch Life Sci, Tempe, AZ USA. RP Barron-Gafford, GA (reprint author), Univ Arizona, Sch Geog & Dev, Tucson, AZ 85721 USA. EM gregbg@email.arizona.edu FU Philecology Foundation of Fort Worth, Texas; Department of Energy National Institute for Climate Change Research; National Science Foundation [DEB-0414680]; University of Arizona Water, Environmental and Energy Solutions program through the Technology and Research Initiative Fund; Center for Environmental Biology at the University of California, Irvine FX This work was supported by the Philecology Foundation of Fort Worth, Texas, a Department of Energy National Institute for Climate Change Research awarded to K.O., a National Science Foundation award (DEB-0414680) to T.H. and R.S., the University of Arizona Water, Environmental and Energy Solutions program through the Technology and Research Initiative Fund, and the Center for Environmental Biology at the University of California, Irvine. NR 71 TC 7 Z9 7 U1 3 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD APR PY 2014 VL 202 IS 2 BP 442 EP 454 DI 10.1111/nph.12675 PG 13 WC Plant Sciences SC Plant Sciences GA AD2JH UT WOS:000333060500017 PM 24417567 ER PT J AU Shiels, AB Pitt, WC Sugihara, RT Witmer, GW AF Shiels, Aaron B. Pitt, William C. Sugihara, Robert T. Witmer, Gary W. TI Biology and Impacts of Pacific Island Invasive Species. 11. Rattus rattus, the Black Rat (Rodentia: Muridae) SO PACIFIC SCIENCE LA English DT Article ID POSTDISPERSAL SEED PREDATION; ANTARCTIC MACQUARIE ISLAND; HAWAIIAN MACADAMIA ORCHARD; MAMMALIAN NEST PREDATORS; INTRODUCED SMALL MAMMALS; OGASAWARA BONIN ISLANDS; MICE MUS MUSCULUS; WESTERN MAUNA-KEA; NEW-ZEALAND; SHIP RATS AB The black rat, roof rat, or ship rat (Rattus rattus L.) is among the most widespread invasive vertebrates on islands and continents, and it is nearly ubiquitous on Pacific islands from the equatorial tropics to approximately 55 degrees latitude north and south. It survives well in human-dominated environments, natural areas, and islands where humans are not present. Rattus rattus is typically the most common invasive rodent in insular forests. Few vertebrates are more problematic to island biota and human livelihoods than R. rattus; it is well known to damage crops and stored foods, kill native species, and serve as a vector for human diseases. Rattus rattus is an omnivore, yet fruit and seed generally dominate its diet, and prey items from the ground to the canopy are commonly at risk and exploited as a result of the prominent arboreal activity of R. rattus. Here we review the biology of this invasive species and its impacts on humans and the insular plants and animals in the Pacific. We also describe some of the past management practices used to control R. rattus populations on islands they have invaded. C1 [Shiels, Aaron B.; Pitt, William C.; Sugihara, Robert T.] APHIS, USDA, Natl Wildlife Res Ctr, Hawaii Field Stn, Hilo, HI 96721 USA. [Witmer, Gary W.] APHIS, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Shiels, AB (reprint author), APHIS, USDA, Natl Wildlife Res Ctr, Hawaii Field Stn, POB 10880, Hilo, HI 96721 USA. EM ashiels@hawaii.edu NR 237 TC 12 Z9 13 U1 9 U2 94 PU UNIV HAWAII PRESS PI HONOLULU PA 2840 KOLOWALU ST, HONOLULU, HI 96822 USA SN 0030-8870 EI 1534-6188 J9 PAC SCI JI Pac. Sci. PD APR PY 2014 VL 68 IS 2 BP 145 EP 184 DI 10.2984/68.2.1 PG 40 WC Marine & Freshwater Biology; Zoology SC Marine & Freshwater Biology; Zoology GA AH6JY UT WOS:000336238400001 ER PT J AU Schulten, JR Cole, TC Cordell, S Publico, KM Ostertag, R Enoka, JE Michaud, JD AF Schulten, Jodie R. Cole, T. Colleen Cordell, Susan Publico, Keiko M. Ostertag, Rebecca Enoka, Jaime E. Michaud, Jene D. TI Persistence of Native Trees in an Invaded Hawaiian Lowland Wet Forest: Experimental Evaluation of Light and Water Constraints SO PACIFIC SCIENCE LA English DT Article ID TROPICAL RAIN-FOREST; PROPAGULE PRESSURE; INVASIVE PLANTS; METROSIDEROS-POLYMORPHA; RELATIVE IMPORTANCE; ECOSYSTEM PROCESSES; BIOMASS ALLOCATION; USE EFFICIENCY; ALIEN PLANTS; COSTA-RICA AB Hawaiian lowland wet forests are heavily invaded and their restoration is most likely to be successful if native species selected for restoration have efficient resource-use traits. We evaluated growth, survival, and ecophysiological responses of four native and four invasive species in a greenhouse experiment that simulated reduced light and water conditions commonly found in invaded field conditions. Our results show that light is a more important limiting resource than water for all species. Specifically, values for photosynthesis, light compensation point, light saturation point, stomatal conductance, leaf mass per area, relative growth rate, and photosynthetic nitrogen use efficiency were all greater under high-light conditions than they were under low-light conditions. In contrast, water limitation negatively affected only stomatal conductance and delta C-13. Our results also show that responses to light were species-specific rather than related to whether species were native or nonnative. We also tested restoration potential of top-performing native species under field conditions in a Hawaiian lowland wet forest by comparing relative growth and mortality rates in both invaded (low-light) plots and in plots from which invasive species had been removed (high-light conditions). Of the native species, Myrsine lessertiana and Psychotria hawaiiensis had highest survival and growth rates in low-light plots after 4 yr, and Metrosideros polymorpha showed 100% mortality under the same conditions. Under low light, M. lessertiana and P. hawaiiensis survived and grew at rates similar to those of invasive species in both field and greenhouse and thus represent suitable candidates for restoration in invaded Hawaiian lowland wet forests. C1 [Schulten, Jodie R.; Publico, Keiko M.; Ostertag, Rebecca; Enoka, Jaime E.] Univ Hawaii, Dept Biol Trop Conservat Biol & Environm Sci, Hilo, HI 96720 USA. [Cole, T. Colleen] Three Mt Alliance, Volcano, HI 96718 USA. [Cordell, Susan] US Forest Serv, Inst Pacific Isl Forestry, USDA, Hilo, HI 96720 USA. [Michaud, Jene D.] Univ Hawaii, Dept Geol, Hilo, HI 96720 USA. RP Schulten, JR (reprint author), Univ Hawaii, Dept Biol Trop Conservat Biol & Environm Sci, Hilo, HI 96720 USA. EM schulten@hawaii.edu FU NSF EPSCoR [0237065, 0554657]; NSF TCUP [0223040]; NSF CAREER [0546868] FX This research was funded and facilitated through NSF EPSCoR Awards 0237065 and 0554657, NSF TCUP 0223040, and NSF CAREER 0546868. Manuscript accepted 16 August 2013. NR 70 TC 5 Z9 5 U1 4 U2 19 PU UNIV HAWAII PRESS PI HONOLULU PA 2840 KOLOWALU ST, HONOLULU, HI 96822 USA SN 0030-8870 EI 1534-6188 J9 PAC SCI JI Pac. Sci. PD APR PY 2014 VL 68 IS 2 BP 267 EP 285 DI 10.2984/68.2.7 PG 19 WC Marine & Freshwater Biology; Zoology SC Marine & Freshwater Biology; Zoology GA AH6JY UT WOS:000336238400007 ER PT J AU Milner, DS Till, R Cadby, I Lovering, AL Basford, SM Saxon, EB Liddell, S Williams, LE Sockett, RE AF Milner, David S. Till, Rob Cadby, Ian Lovering, Andrew L. Basford, Sarah M. Saxon, Emma B. Liddell, Susan Williams, Laura E. Sockett, R. Elizabeth TI Ras GTPase-Like Protein MglA, a Controller of Bacterial Social-Motility in Myxobacteria, Has Evolved to Control Bacterial Predation by Bdellovibrio SO PLOS GENETICS LA English DT Article ID MYXOCOCCUS-XANTHUS; GLIDING MOTILITY; OUTER-MEMBRANE; COGNATE GAP; IV PILI; BACTERIOVORUS; SYSTEM; PREY; COMPLEX; GROWTH AB Bdellovibrio bacteriovorus invade Gram-negative bacteria in a predatory process requiring Type IV pili (T4P) at a single invasive pole, and also glide on surfaces to locate prey. Ras-like G-protein MglA, working with MglB and RomR in the deltaproteobacterium Myxococcus xanthus, regulates adventurous gliding and T4P-mediated social motility at both M. xanthus cell poles. Our bioinformatic analyses suggested that the GTPase activating protein (GAP)-encoding gene mglB was lost in Bdellovibrio, but critical residues for MglA(Bd) GTP-binding are conserved. Deletion of mglA(Bd) abolished prey-invasion, but not gliding, and reduced T4P formation. MglA(Bd) interacted with a previously uncharacterised tetratricopeptide repeat (TPR) domain protein Bd2492, which we show localises at the single invasive pole and is required for predation. Bd2492 and RomR also interacted with cyclic-di-GMP-binding receptor CdgA, required for rapid prey-invasion. Bd2492, RomR(Bd) and CdgA localize to the invasive pole and may facilitate MglA-docking. Bd2492 was encoded from an operon encoding a TamAB-like secretion system. The TamA protein and RomR were found, by gene deletion tests, to be essential for viability in both predatory and non-predatory modes. Control proteins, which regulate bipolar T4P-mediated social motility in swarming groups of deltaproteobacteria, have adapted in evolution to regulate the anti-social process of unipolar prey-invasion in the "lone-hunter" Bdellovibrio. Thus GTP-binding proteins and cyclic-di-GMP inputs combine at a regulatory hub, turning on prey-invasion and allowing invasion and killing of bacterial pathogens and consequent predatory growth of Bdellovibrio. C1 [Milner, David S.; Till, Rob; Basford, Sarah M.; Saxon, Emma B.; Sockett, R. Elizabeth] Univ Nottingham, Sch Med, Sch Life Sci, Ctr Genet & Genom, Nottingham, England. [Cadby, Ian; Lovering, Andrew L.] Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England. [Liddell, Susan] Univ Nottingham, Sch Biosci, Loughborough, Notts, England. [Williams, Laura E.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Athens, GA USA. RP Milner, DS (reprint author), Univ Nottingham, Sch Med, Sch Life Sci, Ctr Genet & Genom, Nottingham, England. EM liz.sockett@nottingham.ac.uk FU BBSRC UK [BB/F016999/1]; NERC UK [NE/I528469/1]; BBSRC [BB/J015229/1]; USDA; Higher Education Funding Council of England FX DSM was funded by a quota PhD studentship BB/F016999/1 to RES from BBSRC UK, SMB was funded by a quota PhD studentship NE/I528469/1 to RES from NERC UK. EBS was an undergraduate student at University of Nottingham. IC was funded by BBSRC grant BB/J015229/1 to ALL and RES. LEW was funded by the USDA. RES, ALL and RT were funded by the Higher Education Funding Council of England which funds UK university staff salaries. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 50 TC 11 Z9 11 U1 3 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7390 EI 1553-7404 J9 PLOS GENET JI PLoS Genet. PD APR PY 2014 VL 10 IS 4 AR e1004253 DI 10.1371/journal.pgen.1004253 PG 13 WC Genetics & Heredity SC Genetics & Heredity GA AG6AB UT WOS:000335499600025 PM 24721965 ER PT J AU Povak, NA Hessburg, PF McDonnell, TC Reynolds, KM Sullivan, TJ Salter, RB Cosby, BJ AF Povak, Nicholas A. Hessburg, Paul F. McDonnell, Todd C. Reynolds, Keith M. Sullivan, Timothy J. Salter, R. Brion Cosby, Bernard J. TI Machine learning and linear regression models to predict catchment- level base cation weathering rates across the southern Appalachian Mountain region, USA SO WATER RESOURCES RESEARCH LA English DT Article DE base cation weathering; machine learning; sulfur; acid deposition; critical loads; regression ID SPECIES DISTRIBUTION MODELS; CONTERMINOUS UNITED-STATES; CRITICAL LOADS; CLIMATE-CHANGE; ACIDIC DEPOSITION; SURFACE WATERS; RANDOM FORESTS; ORGANIC-ACIDS; SOIL; CHEMISTRY AB Accurate estimates of soil mineral weathering are required for regional critical load (CL) modeling to identify ecosystems at risk of the deleterious effects from acidification. Within a correlative modeling framework, we used modeled catchment-level base cation weathering (BCw) as the response variable to identify key environmental correlates and predict a continuous map of BCw within the southern Appalachian Mountain region. More than 50 initial candidate predictor variables were submitted to a variety of conventional and machine learning regression models. Predictors included aspects of the underlying geology, soils, geomorphology, climate, topographic context, and acidic deposition rates. Low BCw rates were predicted in catchments with low precipitation, siliceous lithology, low soil clay, nitrogen and organic matter contents, and relatively high levels of canopy cover in mixed deciduous and coniferous forest types. Machine learning approaches, particularly random forest modeling, significantly improved model prediction of catchment-level BCw rates over traditional linear regression, with higher model accuracy and lower error rates. Our results confirmed findings from other studies, but also identified several influential climatic predictor variables, interactions, and nonlinearities among the predictors. Results reported here will be used to support regional sulfur critical loads modeling to identify areas impacted by industrially derived atmospheric S inputs. These methods are readily adapted to other regions where accurate CL estimates are required over broad spatial extents to inform policy and management decisions. C1 [Povak, Nicholas A.; Hessburg, Paul F.; Salter, R. Brion] US Forest Serv, Pacific NW Res Stn, Wenatchee Forestry Sci Lab, Wenatchee, WA 98801 USA. [McDonnell, Todd C.; Sullivan, Timothy J.] E&S Environm Chem Inc, Corvallis, OR USA. [Reynolds, Keith M.] US Forest Serv, Pacific NW Res Stn, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. [Cosby, Bernard J.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22903 USA. RP Povak, NA (reprint author), US Forest Serv, Pacific NW Res Stn, Wenatchee Forestry Sci Lab, Wenatchee, WA 98801 USA. EM npovak@fs.fed.us RI Cosby, Bernard/B-5653-2012; OI Reynolds, Keith/0000-0002-5286-4754 FU U.S. EPA FX The authors would like to thank the two anonymous reviewers who contributed valuable input into the revisions of this manuscript. We would also like to acknowledge the U.S. EPA for providing the funding for this project. NR 73 TC 5 Z9 5 U1 4 U2 21 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD APR PY 2014 VL 50 IS 4 BP 2798 EP 2814 DI 10.1002/2013WR014203 PG 17 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AH3LV UT WOS:000336026000003 ER PT J AU Luce, C Staab, B Kramer, M Wenger, S Isaak, D McConnell, C AF Luce, Charles Staab, Brian Kramer, Marc Wenger, Seth Isaak, Dan McConnell, Callie TI Sensitivity of summer stream temperatures to climate variability in the Pacific Northwest SO WATER RESOURCES RESEARCH LA English DT Article DE differential sensitivity; climate change; adaptation; stream temperature ID COLUMBIA RIVER-BASIN; WESTERN UNITED-STATES; WATER TEMPERATURE; AIR-TEMPERATURE; BRITISH-COLUMBIA; CHINOOK SALMON; SIERRA-NEVADA; NATIVE FISHES; WILDFIRE; HABITAT AB Estimating the thermal response of streams to a warming climate is important for prioritizing native fish conservation efforts. While there are plentiful estimates of air temperature responses to climate change, the sensitivity of streams, particularly small headwater streams, to warming temperatures is less well understood. A substantial body of literature correlates subannual scale temperature variations in air and stream temperatures driven by annual cycles in solar angle; however, these may be a low-precision proxy for climate change driven changes in the stream energy balance. We analyzed summer stream temperature records from forested streams in the Pacific Northwest for interannual correlations to air temperature and standardized annual streamflow departures. A significant pattern emerged where cold streams always had lower sensitivities to air temperature variation, while warm streams could be insensitive or sensitive depending on geological or vegetation context. A pattern where cold streams are less sensitive to direct temperature increases is important for conservation planning, although substantial questions may yet remain for secondary effects related to flow or vegetation changes induced by climate change. C1 [Luce, Charles; Isaak, Dan] US Forest Serv Res & Dev, Boise, ID 83702 USA. [Staab, Brian] US Forest Serv Pacific Northwest Reg, Portland, OR USA. [Kramer, Marc] Univ Florida, Dept Soil & Water Sci, Gainesville, FL USA. [Wenger, Seth] Univ Georgia, River Basin Ctr, Athens, GA 30602 USA. [McConnell, Callie] US Forest Serv, Washington Off, Washington, DC 20250 USA. RP Luce, C (reprint author), US Forest Serv Res & Dev, Boise, ID 83702 USA. EM cluce@fs.fed.us RI Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 NR 70 TC 17 Z9 17 U1 1 U2 28 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD APR PY 2014 VL 50 IS 4 BP 3428 EP 3443 DI 10.1002/2013WR014329 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AH3LV UT WOS:000336026000038 ER PT J AU Schutte, BJ Davis, AS AF Schutte, Brian J. Davis, Adam S. TI Do Common Waterhemp (Amaranthus rudis) Seedling Emergence Patterns Meet Criteria for Herbicide Resistance Simulation Modeling? SO WEED TECHNOLOGY LA English DT Article DE Emergence modeling; maternal effects; seed germination; seedbank persistence; variance partitioning ID GLYPHOSATE RESISTANCE; INHIBITING HERBICIDES; EMPIRICAL-EVIDENCE; WEED POPULATION; GIANT FOXTAIL; EVOLUTION; TUBERCULATUS; GERMINATION; PALMERI; MANAGEMENT AB A study was conducted to quantify the magnitude and sources of variation in common waterhemp temporal patterns of emergence over 1 yr. In 2008 and 2010, emergence patterns in the absence of soil disturbance were determined for replicated samples of maternal families (progeny from one individual) separately harvested during the previous year from four plants within each of four agricultural fields (16 maternal families yr(-1)) at a university research farm near Urbana, IL. Combining data across years, variance partitioning indicated that seed sample within maternal family explained 48% of total variation in the percentage of viable, buried seeds that produced seedlings. Differences within, rather than among, maternal families also accounted for large fractions (60 to 99%) of total variation in cumulative percentage emergence at specific points during the growing season. Within years, seed samples characterized by delayed or accelerated emergence patterns did not originate from specific maternal plants. These results indicate that common waterhemp seed populations are without strong maternal plant effects that limit emergence to narrow intervals within the overall emergence period. Thus, results of this study support the use of contemporary approaches for modeling herbicide resistance evolution in common waterhemp, which assume seedling cohorts contain offspring from all individuals occurring within the maternal population. C1 [Schutte, Brian J.; Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Schutte, BJ (reprint author), New Mexico State Univ, Dept Entomol Plant Pathol & Weed Sci, Las Cruces, NM 88003 USA. EM bschutte@nmsu.edu FU USDA Agricultural Research Service; U.S. Department of Agriculture FX This work was supported by the USDA Agricultural Research Service. Mention of trade names or commercial products in this article is solely for the purpose of providing scientific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 44 TC 3 Z9 3 U1 5 U2 17 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2014 VL 28 IS 2 BP 408 EP 417 DI 10.1614/WT-D-13-00139.1 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AH4QG UT WOS:000336112500015 ER PT J AU Young, FL Ogg, AG Alldredge, JR AF Young, Frank L. Ogg, Alex G., Jr. Alldredge, J. Richard TI Postharvest Tillage Reduces Downy Brome (Bromus tectorum L.) Infestations in Winter Wheat SO WEED TECHNOLOGY LA English DT Article DE No-till; postharvest tillage; seedbank ID PACIFIC-NORTHWEST; NO-TILL; CROPPING SYSTEMS; ANNUAL GRASSES; WEED SEED; SOIL; DYNAMICS; REGION; DEPTH AB In the Pacific Northwest, downy brome continues to infest winter wheat, especially in low-rainfall areas where the winter wheat summer fallow rotation is the dominant production system. In Washington, a study was conducted for 2 yr at two locations in the winter wheat summer fallow region to determine the influence of four postharvest tillage treatments on vertical seed movement, seedbank depletion, and plant densities of downy brome. The four tillage implements included a disk, sweep plow, harrow, and skew treader. The study also included a no-till treatment for comparison. The sweep plow and disk led to the most vertical movement of downy brome seed compared with the no-till treatment. Approximately 75% of the fall postharvest seed in the no-till treatment was located either on the soil surface or in the 0- to 3-cm depth at both locations. In contrast, 75 % of the seed in the disked treatment was located from 0 to 6 cm deep at both locations. The disk and sweep plow both decreased downy brome seed in the soil at the 0- to 3-cm depth compared with the harrow and no-till treatments. There was no difference in downy brome plant densities following postharvest tillage in the summer fallow due to any of the treatments. However, plant densities in the subsequent winter wheat crop were reduced by the disk and sweep plow compared with the no-till and skew-treader treatments. In general, seed densities as affected by the skew treader fell between the disk and the no-till treatments. The use of the sweep plow and the disk should be integrated into a weed management strategy for downy brome in the wheat fallow region of the Pacific Northwest. C1 [Alldredge, J. Richard] Washington State Univ, Dept Crop & Soil Sci, USDA ARS, Pullman, WA 99164 USA. USDA ARS, Ten Sleep, WY 82442 USA. Washington State Univ, Dept Stat, Pullman, WA 99164 USA. RP Alldredge, JR (reprint author), Washington State Univ, Dept Crop & Soil Sci, USDA ARS, POB 646420, Pullman, WA 99164 USA. EM youngfl@wsu.edu NR 24 TC 1 Z9 1 U1 2 U2 12 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2014 VL 28 IS 2 BP 418 EP 425 DI 10.1614/WT-D-13-00151.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AH4QG UT WOS:000336112500016 ER PT J AU Shaner, D Hager, A AF Shaner, Dale Hager, Aaron TI Detecting and Confirming Accelerated Atrazine Dissipation in Illinois SO WEED TECHNOLOGY LA English DT Article DE Enhanced dissipation; herbicide dissipation ID DEGRADATION; COLORADO; SOIL AB Enhanced dissipation of atrazine has been documented in many parts of the world where the herbicide has been extensively used. Atrazine is widely used in corn in Illinois, but enhanced dissipation in the field has not been documented. In this study, the dissipation of atrazine was measured in three fields. Two of the fields (URB-1 and URB-2) had not been treated with atrazine for 3 and 4 yr, respectively, whereas the third field (ORR-1) had received annual applications of atrazine for 9 yr. A laboratory assay conducted on soil collected from these fields prior to atrazine application indicated that soil from ORR-1 had enhanced atrazine dissipation (half life [DT50] 1.7 d). Soil from fields URB-1 and URB-2 dissipated atrazine much slower (DT50 was 13 and 11d, respectively). In the field, the rates of atrazine dissipation were similar to those measured in the laboratory. The DT(50)s were 2.4, 8.4, and 12.6 d for ORR-1, URB-2, and URB-1, respectively. Testing the soils collected from the fields at the last sampling time for atrazine dissipation showed that all of them had enhanced atrazine dissipation, suggesting that the soil microbial populations had adapted to the presence of atrazine in the field. C1 [Shaner, Dale] USDA ARS, Water Management Res, Ft Collins, CO 80526 USA. [Hager, Aaron] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Shaner, D (reprint author), USDA ARS, Water Management Res, 2150 Ctr Ave,Bldg D, Ft Collins, CO 80526 USA. EM dale.shaner@ars.usda.gov NR 5 TC 2 Z9 2 U1 2 U2 6 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2014 VL 28 IS 2 BP 432 EP 434 DI 10.1614/WT-D-13-00108.1 PG 3 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AH4QG UT WOS:000336112500018 ER PT J AU Harrington, TB AF Harrington, Timothy B. TI Synthetic Auxin Herbicides Control Germinating Scotch Broom (Cytisus scoparius) SO WEED TECHNOLOGY LA English DT Article DE Seedbank; seedling emergence; mortality; biomass ID THISTLE CIRSIUM-ARVENSE; BIOLOGICAL-CONTROL; WASHINGTON; SHRUBS; GROWTH AB Scotch broom is a large, nonnative shrub that has invaded forests and grasslands in 27 U.S. states. Without treatment, Scotch broom's persistent seedbank ensures a continuing source of regeneration after soil disturbance. In growth chamber studies, five rates of three synthetic auxin herbicides, aminocyclopyrachlor (AC), aminopyralid (AP), and dopyralid (CP), were compared for PRE control of Scotch broom. Cumulative 90-d emergence, mortality, and biomass of seedlings did not vary among herbicides, averaging 42 % of seeds sown, 75 % of emerged seedlings, and 9 mg seedling(-1) for treated containers, respectively, versus 46%, 17%, and 26 mg seedling(-1) for nontreated containers. Low rates of each herbicide (< 50% of the maximum labeled rate [MLR]) provided 60 to 80 % control, whereas 100 % MLR provided 69 to 89 % control. Although the herbicides differed in cost per unit seedling mortality (AP < CP < AC), each demonstrated strong potential as a viable treatment for PRE control of Scotch broom. C1 USDA ARS, Pacific NW Res Stn, Olympia, WA 98512 USA. RP Harrington, TB (reprint author), USDA ARS, Pacific NW Res Stn, 3625 93rd Ave SW, Olympia, WA 98512 USA. EM tharrington@fs.fed.us FU Dow AgroSciences LLC FX Financial support for this research was generously provided by Dow AgroSciences LLC. The author is grateful to Vanelle Peterson, Dow AgroSciences LLC, and Kent Pittard, DuPont Company, for providing treatment recommendations, manuscript reviews, and herbicide products. Thanks also to Rob Slesak, University of Minnesota, for reviewing an earlier version of the manuscript and to Bruce Alber, Wilbur-Ellis Company, for providing herbicide cost estimates. The use of trade or firm names in this publication is for reader information and does not imply endorsement by the U.S. Department of Agriculture of any product or service. NR 27 TC 1 Z9 1 U1 5 U2 8 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0890-037X EI 1550-2740 J9 WEED TECHNOL JI Weed Technol. PD APR-JUN PY 2014 VL 28 IS 2 BP 435 EP 442 DI 10.1614/WT-D-13-00170.1 PG 8 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AH4QG UT WOS:000336112500019 ER PT J AU Li, ZD Yao, CH Yu, YH Cai, ZY Wang, XD AF Li, Zhaodong Yao, Chunhua Yu, Yanhao Cai, Zhiyong Wang, Xudong TI Highly Efficient Capillary Photoelectrochemical Water Splitting Using Cellulose Nanofi ber- Templated TiO 2 Photoanodes SO ADVANCED MATERIALS LA English DT Article DE cellulose nanofibers; TiO2; capillary; photoelectrochemistry; water splitting ID ATOMIC LAYER DEPOSITION; NANOWIRE ARCHITECTURE; HYDROGEN GENERATION; COMPOSITE ELECTRODE; ENERGY-CONVERSION; SOLAR-ENERGY; REPLICATION; OXIDE C1 [Li, Zhaodong; Yao, Chunhua; Yu, Yanhao; Wang, Xudong] Univ Wisconsin, Dept Mat Sci & Engn, Madison, WI 53706 USA. [Yao, Chunhua; Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. RP Cai, ZY (reprint author), US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53726 USA. EM zcai@fs.fed.us; xudong@engr.wisc.edu RI Wang, Xudong/A-7067-2009 FU Wisconsin Alumni Research Foundation (WARF); U.S. Department of Energy, Office of Basic Energy Sciences [DE-SC0008711] FX The authors offer thanks to Wisconsin Alumni Research Foundation (WARF) and U.S. Department of Energy, Office of Basic Energy Sciences under Award DE-SC0008711, for financial support. NR 39 TC 32 Z9 34 U1 14 U2 155 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 0935-9648 EI 1521-4095 J9 ADV MATER JI Adv. Mater. PD APR PY 2014 VL 26 IS 14 BP 2262 EP 2267 DI 10.1002/adma.201303369 PG 6 WC Chemistry, Multidisciplinary; Chemistry, Physical; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter SC Chemistry; Science & Technology - Other Topics; Materials Science; Physics GA AF0IV UT WOS:000334398400009 PM 24615860 ER PT J AU Erickson, HE Helmer, EH Brandeis, TJ Lugo, AE AF Erickson, Heather E. Helmer, E. H. Brandeis, Thomas J. Lugo, Ariel E. TI Controls on fallen leaf chemistry and forest floor element masses in native and novel forests across a tropical island SO ECOSPHERE LA English DT Article DE C/N ratio; forest floor mass; landscape analyses; leaf litter chemistry; N/P ratio; novel forests; precipitation effects; stand age; stoichiometry; subtropical forests; tropical legumes; variance analysis ID SYMBIOTIC NITROGEN-FIXATION; N-P RATIOS; PUERTO-RICO; RAIN-FOREST; NUTRIENT LIMITATION; DRY FOREST; SPATIAL-PATTERNS; LAND-USE; PHOSPHORUS LIMITATION; LITTER DECOMPOSITION AB Litter chemistry varies across landscapes according to factors rarely examined simultaneously. We analyzed 11 elements in forest floor (fallen) leaves and additional litter components from 143 forest inventory plots systematically located across Puerto Rico, a tropical island recovering from large-scale forest clearing. We assessed whether three existing, independently-derived, landscape classifications (Holdridge life zone, remotely sensed forest type (leaf longevity combined with geology generalized to karst vs. non-karst), and plot-based measures of forest assemblage) would separate observed gradients. With principal component and regression analyses, we also tested whether climate-, landscape-(geology, elevation, aspect, percent slope, slope position, distance from coast), and stand-scale (tree species composition, basal area, density, stand age) variables explained variation in fallen leaf chemistry and stoichiometry. For fallen leaves, C, Ca, Mg, Na, and Mn concentrations differed by Holdridge life zone and C, P, Ca, Mn, Al, and Fe concentrations differed by forest type, where leaf longevity distinguished C and Ca concentrations and geology distinguished C, P, Ca, Mn, Al, and Fe concentrations. Fallen leaf C, P, Ca, and Mn concentrations also differed, and N concentrations only differed, by forest assemblage. Across several scales, fallen leaf N concentration was positively related to the basal area of putatively N2-fixing tree legumes, which were concentrated in lower topographic positions, providing for the first time a biological explanation for the high N concentrations of fallen leaves in these locations. Phosphorus concentrations in fallen leaves by forest assemblages also correlated with the basal area of N2-fixing legumes, and P and N concentrations decreased with mean age of assemblage. Fallen leaves from younger (, 50 yr, 86% of the plots) and often novel forests had higher P, Fe, and Al and lower C concentrations and lower C/P and N/P ratios than fallen leaves from older forests, the latter due to a decrease in P rather than changes in N. These findings suggest that both N and P availability may currently be greater on the island than pre-deforestation, and substantiate the unique roles that state factors play in contributing to the spatial heterogeneity of fallen leaf chemistry. C1 [Erickson, Heather E.] USDA Forest Serv, Pacific NW Res Stn, Portland, OR 97205 USA. [Helmer, E. H.; Lugo, Ariel E.] USDA Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR 00926 USA. [Brandeis, Thomas J.] USDA Forest Serv, Southern Res Stn, Knoxville, TN 37919 USA. RP Erickson, HE (reprint author), USDA Forest Serv, Pacific NW Res Stn, Portland, OR 97205 USA. EM ericksonheather@yahoo.com FU International Institute of Tropical Forestry (IITF); Southern Research Station of the USDA Forest Service FX The FIA Program in Puerto Rico is jointly funded and conducted by the International Institute of Tropical Forestry (IITF) and the Southern Research Station of the USDA Forest Service. We thank Mary J. Sanchez and the lab at IITF for analyzing litter samples and the IITF and FIA field crews, especially Humfredo Marcano, Luis Ortiz-Rivera and Ivan Vicens, for their collection. Ernesto Medina, Becky Ostertag, Steven Perakis, Philip Sollins, and several anonymous reviewers are thanked for their critiques. Pat Cunningham and the Statistics Unit at the PNW Research Station provided valuable advice. This work was done in cooperation with the University of Puerto Rico. NR 116 TC 2 Z9 2 U1 3 U2 19 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2014 VL 5 IS 4 AR 48 DI 10.1890/ES13-00263.1 PG 28 WC Ecology SC Environmental Sciences & Ecology GA AH2ML UT WOS:000335955500002 ER PT J AU Ulyshen, MD Wagner, TL Mulrooney, JE AF Ulyshen, Michael D. Wagner, Terence L. Mulrooney, Joseph E. TI Contrasting effects of insect exclusion on wood loss in a temperate forest SO ECOSPHERE LA English DT Article DE arthropods; biodiversity; coarse woody debris; dead wood; ecosystem services; invertebrates; saproxylic taxa ID FUNGAL SPORE GERMINATION; SUBTERRANEAN TERMITE; UNITED-STATES; DECOMPOSITION; DECAY; ISOPTERA; LITTER; DEBRIS; RHINOTERMITIDAE; RETICULITERMES AB Experimental efforts to determine how insects influence terrestrial wood decomposition are few, especially in temperate regions. To address this need, a five-year exclusion study was conducted in northern Mississippi, U. S. A., to quantify insect contributions to wood decay using one-meter loblolly pine (Pinus taeda L.) bolts. The study included three treatments: (1) "partially protected'' bolts that were placed on cypermethrin-treated soil to exclude subterranean termites (Isoptera: Rhinotermitidae: Reticulitermes spp.) while permitting colonization by beetles (Coleoptera) and other saproxylic taxa, (2) "fully protected'' bolts that were placed on cypermethrin-treated soil and enclosed within screen cages to protect against all insects and (3) "unprotected'' bolts that were not subjected to either exclusion treatment. The full insect community consumed approximately 15-20% of wood volume in unprotected bolts, about six times more than in partially protected bolts from which termites were excluded. There were no differences in specific gravity (based on initial wood volume) or mass loss among treatments, however. It is not clear whether these findings are due to an inhibition of microbial decomposers by insects (e. g., antimicrobial compounds secreted by termites or ants), a stimulatory effect of the exclusion treatments (e. g., cypermethrin stimulating fungal growth or cages favorably altering wood moisture), or some combination of both. When based on final water-displaced volume, specific gravity was significantly higher for unprotected bolts than for those fully protected, probably because termites selectively consume the least dense wood. By the end of the study, about 20% of the final dry weight of unprotected bolts consisted of termite-imported soil. Wood volume consumed and soil content decreased with distance from the ends of the bolts whereas water content exhibited the opposite pattern. We detected a significant negative relationship between water content and volume consumed by termites, possibly because water content decreases with increasing wood density and termites tend to avoid high density wood. While insects clearly consume large volumes of wood in southeastern U. S. forests, our results suggest they do not act to accelerate mass loss beyond what is achieved by microbial decomposers. More research is needed to confirm this, however-especially given the uncertainties inherent to exclusion studies. C1 [Ulyshen, Michael D.; Wagner, Terence L.; Mulrooney, Joseph E.] Forest Serv, USDA, Southern Res Stn, Starkville, MS 39759 USA. RP Ulyshen, MD (reprint author), Forest Serv, USDA, Southern Res Stn, 201 Lincoln Green, Starkville, MS 39759 USA. EM mulyshen@fs.fed.us NR 55 TC 6 Z9 6 U1 5 U2 42 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2014 VL 5 IS 4 AR 47 DI 10.1890/ES13-00365.1 PG 15 WC Ecology SC Environmental Sciences & Ecology GA AH2ML UT WOS:000335955500001 ER PT J AU Deal, RL Hennon, P O'Hanlon, R D'Amore, D AF Deal, Robert L. Hennon, Paul O'Hanlon, Richard D'Amore, David TI Lessons from native spruce forests in Alaska: managing Sitka spruce plantations worldwide to benefit biodiversity and ecosystem services SO FORESTRY LA English DT Review ID TEMPERATE RAIN-FOREST; ECTOMYCORRHIZAL FUNGAL COMMUNITIES; CONTINUOUS COVER FORESTRY; WESTERN HEMLOCK FORESTS; WOOD-INHABITING FUNGI; OLYMPIC NATIONAL-PARK; GREEN-TREE RETENTION; SOUTHEAST ALASKA; PICEA-SITCHENSIS; OLD-GROWTH AB There is increasing interest worldwide in managing forests to maintain or improve biodiversity, enhance ecosystem services and assure long-term sustainability of forest resources. An important goal of forest management is to increase stand diversity, provide wildlife habitat and improve forest species diversity. We synthesize results from natural spruce forests in southeast Alaska and suggest strategies for managing Sitka spruce plantations in other parts of the world to benefit biodiversity and enhance a variety of forest ecosystem services. We also discuss the roles of fungi in increasing both biological and structural diversity in Sitka spruce forests. New silvicultural systems that use partial cutting in older spruce forests could alleviate some of the problems associated with conventional even-aged management and increase both stand structural diversity and biodiversity. We found that mixed red alder-conifer stands in Alaska provided more heterogeneous structures than the pure conifer stands that typically develop after clear cutting. Well-planned silvicultural systems that include broad leaved species such as alder or birch could provide trees for timber production, improve wildlife habitat and a variety of other ecosystem services that are often compromised in young pure conifer forests. C1 [Deal, Robert L.] US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97205 USA. [Hennon, Paul; D'Amore, David] US Forest Serv, USDA, Pacific NW Res Stn, Juneau, AK 99801 USA. [O'Hanlon, Richard] TEAGASC, Plant Hlth Lab, Dept Agr Food & Marine, Celbridge, Kildare, Ireland. RP Deal, RL (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Portland, OR 97205 USA. EM rdeal@fs.fed.us FU Department of Agriculture, Food and the Marine Ireland through the PHYTOFOR project FX R.O.H. is funded by the Department of Agriculture, Food and the Marine Ireland through the PHYTOFOR project. NR 163 TC 5 Z9 6 U1 8 U2 51 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD APR PY 2014 VL 87 IS 2 SI SI BP 193 EP 208 DI 10.1093/forestry/cpt055 PG 16 WC Forestry SC Forestry GA AH1WO UT WOS:000335912700002 ER PT J AU Coulston, JW Reams, GA Wear, DN Brewer, CK AF Coulston, John W. Reams, Gregory A. Wear, David N. Brewer, C. Kenneth TI An analysis of forest land use, forest land cover and change at policy-relevant scales SO FORESTRY LA English DT Article ID ACCURACY ASSESSMENT; WORLDS FORESTS; UNITED-STATES; PLANTATIONS; AREA AB Quantifying the amount of forest and change in the amount of forest are key to ensure that appropriate management practices and policies are in place to maintain the array of ecosystem services provided by forests. There are a range of analytical techniques and data available to estimate these forest parameters, however, not all 'forest' is the same and various components of change have been presented. Forest as defined by use and forest as defined by cover are different, although it is common for scientists and policy makers to infer one from the other. We compare and contrast estimates of forest land cover, forest land use, extent and change at policy-relevant scales in the southeastern US. We found that estimates of forest land use extent and forest land cover extent were not significantly correlated. Estimates of net change based on forest land cover and forest land use were only moderately correlated and net change estimates were independent of gross forest cover loss estimates. C1 [Coulston, John W.] US Forest Serv, USDA, Knoxville, TN 37919 USA. [Reams, Gregory A.; Brewer, C. Kenneth] US Forest Serv, USDA, Arlington, VA 22209 USA. [Wear, David N.] N Carolina State Univ, US Forest Serv, USDA, Raleigh, NC 27695 USA. RP Coulston, JW (reprint author), US Forest Serv, USDA, 4700 Old Kingston Pike, Knoxville, TN 37919 USA. EM jcoulston@fs.fed.us NR 30 TC 10 Z9 11 U1 1 U2 19 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD APR PY 2014 VL 87 IS 2 SI SI BP 267 EP 276 DI 10.1093/forestry/cpt056 PG 10 WC Forestry SC Forestry GA AH1WO UT WOS:000335912700009 ER PT J AU Lee, S Pan, H Hse, CY Gunasekaran, AR Shupe, TF AF Lee, Sangyeob Pan, Hui Hse, Chung Y. Gunasekaran, Alfred R. Shupe, Todd F. TI Characteristics of regenerated nanocellulosic fibers from cellulose dissolution in aqueous solutions for wood fiber/polypropylene composites SO JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS LA English DT Article DE Aqueous solutions; regenerated cellulose; biocomposites; nanofibers ID NAOH/UREA SOLUTION; TEMPERATURE; BEHAVIOR AB The effects of aqueous solutions were evaluated on the properties of regenerated cellulosic nanofibers prepared from pure cellulose fibers in various formulations of aqueous solutions. Thermoplastic composites were prepared with reinforcement of the regenerated cellulosic nanofibers. The regenerated cellulosic fibers from cellulosic woody biomass were obtained from dissolved cellulose solutions by coagulating with sulfuric acid and water for phase separation. The properties of the regenerated cellulosic fibers were characterized using Fourier-transform infrared spectroscopy (FTIR), wide angle X-ray diffraction (WAXRD), thermogravimetric analysis (TGA), field emission scanning electron microscopy, and tensile testing. The TGA, WAXRD, and FTIR spectra indicated that the regenerated nanofibers possessed cellulosic crystal type II. The micrographs of regenerated cellulosic fibers showed a dense composite structure and lower crystallinity than controlled fibers. The tensile strength of regenerated cellulosic fiber-reinforced polymer composites reached 30MPa, which was 70% higher than the control fiber-reinforced composites. The composites prepared from regenerated fibers with sodium hydroxide (NaOH)/urea and NaOH/urea/thiourea aqueous solutions provided the best results. This work also provides a potential promising method to efficiently obtain nanocellulosic fibers as reinforcement materials in bio-based nanocomposites. C1 [Lee, Sangyeob] Mississippi Pacific Resins Inc, Starkville, MS 39760 USA. [Pan, Hui] Louisiana State Univ, Ctr Agr, Calhoun Res Stn, Calhoun, LA USA. [Hse, Chung Y.] US Forest Serv, Southern Res Stn, USDA, Pineville, LA USA. [Gunasekaran, Alfred R.] Louisiana Tech Univ, Inst Micromfg, Ruston, LA 71270 USA. [Shupe, Todd F.] Louisiana State Univ, AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. RP Lee, S (reprint author), Mississippi Pacific Resins Inc, POB 412, Starkville, MS 39760 USA. EM lsy6709.mpresin@gmail.com NR 23 TC 1 Z9 1 U1 2 U2 18 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0892-7057 EI 1530-7980 J9 J THERMOPLAST COMPOS JI J. Thermoplast. Compos. Mater. PD APR PY 2014 VL 27 IS 4 BP 558 EP 570 DI 10.1177/0892705713484739 PG 13 WC Materials Science, Composites SC Materials Science GA AC2TS UT WOS:000332357500009 ER PT J AU Hollinger, K Yang, CX Montz, RE Nonneman, D Ross, JW Selsby, JT AF Hollinger, Katrin Yang, Cai X. Montz, Robyn E. Nonneman, Dan Ross, Jason W. Selsby, Joshua T. TI Dystrophin insufficiency causes selective muscle histopathology and loss of dystrophin-glycoprotein complex assembly in pig skeletal muscle SO FASEB JOURNAL LA English DT Article DE BMD; animal model; Becker muscular dystrophy; Duchenne muscular dystrophy; DMD ID DUCHENNE MUSCULAR-DYSTROPHY; DEFICIENT MDX MICE; THERAPEUTIC STRATEGIES; MODELS; MUTATION; MOUSE; CONTRACTION; PREDNISONE; DATABASE; RINGO AB The purpose of this investigation was to determine the extent to which dystrophin insufficiency caused histomorphological changes in a novel pig model of Becker muscular dystrophy. In our procedures, we used a combination of biochemical approaches, including quantitative PCR and Western blots, along with a histological analysis using standard and immunohistological measures. We found that 8-wk-old male affected pigs had a 70% reduction in dystrophin protein abundance in the diaphragm, psoas major, and longissimus lumborum and a 5-fold increase in serum creatine kinase activity compared with healthy male littermates. Dystrophin insufficiency in the diaphragm and the longissimus resulted in muscle histopathology with disorganized fibrosis that often colocalized with fatty infiltration but not the psoas. Affected animals also had an 80-85% reduction in alpha-sarcoglycan localization in these muscles, indicating compromised assembly of the dystrophin glycoprotein complex. Controls used in this study were 4 healthy male littermates, as they are most closely related to the affected animals. We concluded that pigs with insufficient dystrophin protein expression have a phenotype consistent with human dystrophinopathy patients. Given that and their similarity in body size and physiology to humans, we further conclude that this pig line is an appropriate translational model for dystrophinopathies.-Hollinger, K., Yang, C. X., Montz, R. E., Nonneman, D., Ross, J. W., Selsby, J. T. Dystrophin insufficiency causes selective muscle histopathology and loss of dystrophin-glycoprotein complex assembly in pig skeletal muscle. C1 [Hollinger, Katrin; Yang, Cai X.; Montz, Robyn E.; Ross, Jason W.; Selsby, Joshua T.] Iowa State Univ, Dept Anim Sci, Ames, IA 50011 USA. [Nonneman, Dan] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE USA. RP Selsby, JT (reprint author), Iowa State Univ, Dept Anim Sci, 2356 Kildee Hall, Ames, IA 50011 USA. EM jselsby@iastate.edu OI Selsby, Joshua/0000-0003-3797-7539 FU U.S. National Institutes of Health [RR-030232, NS-079603] FX The authors are grateful for insights provided by Dr. Ted Huiatt. This project was partially supported by the U.S. National Institutes of Health, grants RR-030232 (to J.W.R.) and NS-079603 (to J.T.S.). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). NR 36 TC 11 Z9 11 U1 0 U2 6 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD APR PY 2014 VL 28 IS 4 BP 1600 EP 1609 DI 10.1096/fj.13-241141 PG 10 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AG3UC UT WOS:000335344300009 PM 24347611 ER PT J AU Sehgal, B Subramanyam, B Arthur, FH Gill, BS AF Sehgal, Blossom Subramanyam, Bhadriraju Arthur, Frank H. Gill, Bikram S. TI Variation in susceptibility of laboratory and field strains of three stored-grain insect species to beta-cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to concrete surfaces SO PEST MANAGEMENT SCIENCE LA English DT Article DE insecticides; empty-bin treatments; stored-grain insects; field strains; efficacy assessment ID RHYZOPERTHA-DOMINICA COLEOPTERA; ORYZAEPHILUS-SURINAMENSIS L; FLOUR BEETLES COLEOPTERA; TEMPERATURE-TOXICITY RELATIONSHIPS; TRIBOLIUM-CASTANEUM COLEOPTERA; GERMAN-COCKROACH DICTYOPTERA; HOUSE-FLIES DIPTERA; WETTABLE POWDER; RESIDUAL EFFICACY; SITOPHILUS-GRANARIUS AB BACKGROUNDThe efficacy of commercial formulations of -cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to clean, concrete surfaces similar to that of empty bins against field strains of stored-grain insects is unknown. We exposed adults of 16 strains of the red flour beetle, Tribolium castaneum (Herbst); eight strains of the sawtoothed grain beetle, Oryzaephilus surinamensis (L.); and two strains of the lesser grain borer, Rhyzopertha dominica (F.), collected mainly from farm-stored grain in Kansas, USA, to -cyfluthrin and chlorpyrifos-methyl plus deltamethrin applied to concrete surfaces and determined knockdown and mortality. RESULTSKnockdown and mortality differences among species and strains to the insecticides tested were significant. Mortality of all species was less than that of knockdown, suggesting recovery when placed on food after insecticide exposure. -Cyfluthrin was effective against R. dominica but ineffective against T. castaneum and O. surinamensis field strains. Chlorpyrifos-methyl plus deltamethrin was only partially effective against field strains of the three species. CONCLUSIONReduced susceptibility in field strains may be due to inherent formulation deficiency and low levels of tolerance or resistance to -cyfluthrin. No single insecticide provided adequate control of the three species tested. (c) 2013 Society of Chemical Industry C1 [Sehgal, Blossom; Subramanyam, Bhadriraju] Kansas State Univ, Dept Grain Sci & Ind, Manhattan, KS 66506 USA. [Arthur, Frank H.] ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. [Gill, Bikram S.] Kansas State Univ, Dept Plant Pathol, Manhattan, KS 66506 USA. RP Subramanyam, B (reprint author), Kansas State Univ, Dept Grain Sci & Ind, 201 Shellenberber Hall, Manhattan, KS 66506 USA. EM sbhadrir@k-state.edu FU Bayer CropScience (Research Triangle Park, NC, USA) FX Bayer CropScience (Research Triangle Park, NC, USA) provided the insecticides and financial support for this research. The authors are grateful to Kansas producers for permitting them to sample their stored grain to collect field strains, and to Dr James Campbell, USDA-ARS, Center for Grain and Animal Health Research, Manhattan, KS, USA, for providing five Tribolium castaneum and one Rhyzopertha dominica field strains. Dr Nickolas Kavallieratos reviewed an earlier version of the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA) or by Kansas State University. USDA is an equal opportunity provider and employer. This paper is contribution number 13-214-J of the Kansas State University Agricultural Experiment Station. NR 54 TC 8 Z9 10 U1 2 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD APR PY 2014 VL 70 IS 4 BP 576 EP 587 DI 10.1002/ps.3580 PG 12 WC Agronomy; Entomology SC Agriculture; Entomology GA AC3CT UT WOS:000332393300009 PM 23674499 ER PT J AU Khatibi, PA McMaster, NJ Musser, R Schmale, DG AF Khatibi, Piyum A. McMaster, Nicole J. Musser, Robert Schmale, David G., III TI Survey of Mycotoxins in Corn Distillers' Dried Grains with Solubles from Seventy-Eight Ethanol Plants in Twelve States in the U.S. in 2011 SO TOXINS LA English DT Article DE mycotoxins; maize; deoxynivalenol; zearalenone; 15-acetyldeoxynivalenol; nivalenol; ethanol; 3-acetyldeoxynivalenol; DDGS ID FUSARIUM-GRAMINEARUM; ACETYLATED DERIVATIVES; NATURAL OCCURRENCE; MASS-SPECTROMETRY; DEOXYNIVALENOL; ZEARALENONE; BARLEY; WHEAT; COPRODUCTS; NIVALENOL AB Fuel ethanol co-products known as distillers' dried grains with solubles (DDGS) are a significant source of energy, protein, and phosphorous in animal feed. Fuel ethanol production may concentrate mycotoxins present in corn into DDGS. One hundred and forty one corn DDGS lots collected in 2011 from 78 ethanol plants located in 12 states were screened for the mycotoxins deoxynivalenol (DON), 15-acetyldeoxynivalenol (15-ADON), 3-acetyldeoxynivalenol (3-ADON), nivalenol (NIV), and zearalenone (ZON). DON ranged from <0.50 to 14.62 mu g g(-1), 15-ADON ranged from <0.10 to 7.55 mu g g(-1), and ZON ranged from <0.10 to 2.12 mu g g(-1). None of the DDGS lots contained 3-ADON or NIV. Plants in OH had the highest levels of DON overall (mean of 9.51 mu g g(-1)), and plants in NY, MI, IN, NE, and WI had mean DON levels >1 and <4 mu g g(-1). Twenty six percent (36/141) of the DDGS lots contained 1.0 to 5.0 mu g g(-1) DON, 2% (3/141) contained >5.0 and <10.0 mu g g(-1) DON, and 3% (4/141) contained >10.0 mu g g(-1) DON. All DDGS lots contaminated with unacceptable levels of DON evaded detection prior to their commercial distribution and were likely sold as feed products. C1 [Khatibi, Piyum A.] ARS, Renewable Prod Technol Res Unit, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [McMaster, Nicole J.; Schmale, David G., III] Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. [Musser, Robert] Nutriquest, Mason City, IA 50401 USA. RP Schmale, DG (reprint author), Virginia Tech, Dept Plant Pathol Physiol & Weed Sci, Blacksburg, VA 24061 USA. EM Piyum.Khatibi@ars.usda.gov; niki@vt.edu; rob@nutriquest.biz; dschmale@vt.edu FU Virginia Small Grains Board [10278306]; U.S. Wheat and Barley Scab Initiative [07185403]; U.S. Department of Agriculture FX We thank Shantal Hover and Zach Upchurch for their assistance with preparing distillers' dried grains with solubles (DDGS) lots for mycotoxin analysis. We would also like to thank Beth Tacke and members of the Mostrom Lab for providing a positive control corn DDGS sample and sharing extraction methods for corn DDGS. This work was supported in part by grants to David Schmale from the Virginia Small Grains Board (10278306) and the U.S. Wheat and Barley Scab Initiative (07185403). This material is based upon work supported by the U.S. Department of Agriculture. This is a cooperative project with the U.S. Wheat and Barley Scab Initiative. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U. S. Department of Agriculture. NR 39 TC 6 Z9 6 U1 0 U2 8 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-6651 J9 TOXINS JI Toxins PD APR PY 2014 VL 6 IS 4 BP 1155 EP 1168 DI 10.3390/toxins6041155 PG 14 WC Toxicology SC Toxicology GA AG9SO UT WOS:000335760200001 PM 24674933 ER PT J AU Lees, RS Knols, B Bellini, R Benedict, MQ Bheecarry, A Bossin, HC Chadee, DD Charlwood, J Dabire, RK Djogbenou, L Egyir-Yawson, A Gato, R Gouagna, LC Hassan, MM Khan, SA Koekemoer, LL Lemperiere, G Manoukis, NC Mozuraitis, R Pitts, RJ Simard, F Gilles, JRL AF Lees, Rosemary Susan Knols, Bart Bellini, Romeo Benedict, Mark Q. Bheecarry, Ambicadutt Bossin, Herve Christophe Chadee, Dave D. Charlwood, Jacques Dabire, Roch K. Djogbenou, Luc Egyir-Yawson, Alexander Gato, Rene Gouagna, Louis Clement Hassan, Mo'awia Mukhtar Khan, Shakil Ahmed Koekemoer, Lizette L. Lemperiere, Guy Manoukis, Nicholas C. Mozuraitis, Raimondas Pitts, R. Jason Simard, Frederic Gilles, Jeremie R. L. TI Review: Improving our knowledge of male mosquito biology in relation to genetic control programmes SO ACTA TROPICA LA English DT Review ID AEDES-ALBOPICTUS DIPTERA; STERILE INSECT TECHNIQUE; MALE ANOPHELES-GAMBIAE; INTEGRATED VECTOR MANAGEMENT; CHIKUNGUNYA OUTBREAK AREA; POPULATION-SIZE SURVEY; ITALIAN URBAN AREAS; RICE-GROWING AREA; FRENCH-POLYNESIA; MALARIA CONTROL AB The enormous burden placed on populations worldwide by mosquito-borne diseases, most notably malaria and dengue, is currently being tackled by the use of insecticides sprayed in residences or applied to bednets, and in the case of dengue vectors through reduction of larval breeding sites or larviciding with insecticides thereof. However, these methods are under threat from, amongst other issues, the development of insecticide resistance and the practical difficulty of maintaining long-term community-wide efforts. The sterile insect technique (SIT), whose success hinges on having a good understanding of the biology and behaviour of the male mosquito, is an additional weapon in the limited arsenal against mosquito vectors. The successful production and release of sterile males, which is the mechanism of population suppression by SIT, relies on the release of mass-reared sterile males able to confer sterility in the target population by mating with wild females. A five year Joint FAO/IAEA Coordinated Research Project brought together researchers from around the world to investigate the pre-mating conditions of male mosquitoes (physiology and behaviour, resource acquisition and allocation, and dispersal), the mosquito mating systems and the contribution of molecular or chemical approaches to the understanding of male mosquito mating behaviour. A summary of the existing knowledge and the main novel findings of this group is reviewed here, and further presented in the reviews and research articles that form this Acta Tropica special issue. Copyright (C) International Atomic Energy Agency 2013. Published by Elsevier B.V. All rights reserved.. C1 [Lees, Rosemary Susan; Gilles, Jeremie R. L.] IAEA, Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, FAO IAEM Agr & Biotechnol Labs, A-1400 Vienna, Austria. [Knols, Bart] In2Care BV, NL-6702 AA Wageningen, Netherlands. [Bellini, Romeo] Ctr Agr Ambiente G Nicoli, I-40014 Crevalcore, Italy. [Benedict, Mark Q.] Polo Innovaz Genom Genet & Biol SCaRL, I-06132 Perugia, Italy. [Bheecarry, Ambicadutt] Minist Hlth & Qual Life, Vector Biol & Control Div, Curepipe, Mauritius. [Bossin, Herve Christophe] Inst Louis Malarde, Lab Rech Entomol Med, Papeete, Tahiti, Fr Polynesia. [Chadee, Dave D.] Univ W Indies, Dept Life Sci, St Augustine, Trinid & Tobago. [Charlwood, Jacques] DBL Inst Hlth Res & Dev, DK-2920 Charlottenlund, Denmark. [Dabire, Roch K.] Ctr Muraz Org Cooperat & Coordinat Lutte Grandes, Bobo Dioulasso, Burkina Faso. [Djogbenou, Luc] Univ Abomey Calavi, Inst Reg Sante Publ LRSP, Cotonou, Benin. [Egyir-Yawson, Alexander] Ghana Atom Energy Commiss, Biotechnol & Nucl Agr Res Inst, Accra, Ghana. [Gato, Rene] Inst Med Trop Pedro Kouri, Havana, Cuba. [Gouagna, Louis Clement; Lemperiere, Guy] Ctr Rech & Veille Malad Emergentes Ocean Indien C, MIVEGEC Malad Infect & Vecteurs Ecol Genet Evolut, UMR 5290 224, Inst Rech Dev, St Clotilde, Reunion. [Hassan, Mo'awia Mukhtar] Res Inst Trop Med, Dept Epidemiol, Khartoum, Sudan. [Khan, Shakil Ahmed] Bangladesh Atom Energy Commiss, Atom Energy Res Estab, Inst Food & Radiat Biol, Dhaka 1344, Bangladesh. [Koekemoer, Lizette L.] Natl Hlth Lab Serv, Natl Inst Communicate Dis, ZA-2131 Johannesburg, South Africa. [Koekemoer, Lizette L.] Univ Witwatersrand, Fac Hlth Sci, Wits Res Inst Malaria, Johannesburg, South Africa. [Manoukis, Nicholas C.] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI USA. [Mozuraitis, Raimondas] Royal Inst Technol, Dept Chem, S-10044 Stockholm, Sweden. [Pitts, R. Jason] Vanderbilt Univ, Dept Biol Sci, Nashville, TN 37232 USA. [Simard, Frederic] Inst Rech Dev, UMR 5290 224, MIVEGEC Malad Infect & Vecteurs Ecol Genet Evol &, F-34394 Montpellier, France. RP Lees, RS (reprint author), Polo Innovaz Genom Genet & Biol SCaRL, Edificio D, I-06132 Perugia, Italy. EM louis-clement.gouagna@ird.fr; bart@in2care.org; rbellini@caa.it; mqbenedict@yahoo.com; abheecarry.vbcd@mail.gov.mu; hbossin@ilm.pf; dave.chadee@sta.uwi.edu; jdcharlwood@gmail.com; dabire_roch@hotmail.com; ldjogbenou22002@yahoo.fr; egyiryawson@hotmail.com; rene@ipk.sld.cu; louis-clement.gouagna@ird.fr; moawia65@hotmail.com; ibd@aitlbd.net; lizettek@nicd.ac.za; guy.lemperiere@ird.fr; nicholas.manoukis@ars.usda.gov; raimis@kth.se; j.pitts@vanderbilt.edu; frederic.simard@ird.fr; j.gilles@iaea.org RI SIMARD, Frederic/J-9489-2016; Knols, Bart/C-5765-2017; OI SIMARD, Frederic/0000-0002-2871-5329; Manoukis, Nicholas/0000-0001-5062-7256; Lees, Rosemary/0000-0002-4232-9125; /0000-0002-1719-2294; BOSSIN, Herve/0000-0002-3655-0923 NR 165 TC 17 Z9 22 U1 2 U2 44 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD APR PY 2014 VL 132 SU S SI SI BP S2 EP S11 DI 10.1016/j.actatropica.2013.11.005 PG 10 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA AG0NK UT WOS:000335112000002 PM 24252487 ER PT J AU Manoukis, NC Butail, S Diallo, M Ribeiro, JMC Paley, DA AF Manoukis, Nicholas C. Butail, Sachit Diallo, Moussa Ribeiro, Jose M. C. Paley, Derek A. TI Stereoscopic video analysis of Anopheles gambiae behavior in the field: Challenges and opportunities SO ACTA TROPICA LA English DT Article DE Anopheles gambiae; Swarming; Mating behavior; Computer vision ID MOTION CORRESPONDENCE; POPULATION-CONTROL; SWARMING BEHAVIOR; MATING-BEHAVIOR; MOSQUITOS; DIPTERA; CULICIDAE; DOMINANT; FITNESS; SYSTEMS AB Advances in our ability to localize and track individual swarming mosquitoes in the field via stereoscopic image analysis have enabled us to test long-standing ideas about individual male behavior and directly observe coupling. These studies further our fundamental understanding of the reproductive biology of mosquitoes. In addition, our analyses using stereoscopic video of swarms of the African malaria vector Anopheles gambiae have produced results that should be relevant to any "release-based" method of control including Sterile Insect Technique (SIT) and genetically modified male mosquitoes (GMM). The relevance of the results is primarily due to the fact that any mosquito vectors released for control are almost certainly going to be males; further, for SIT, GMM or similar approaches to be successful, the released males will have to successfully locate swarms and then mate with wild females. Thus, understanding and potentially manipulating the mating process could play a key role in future control programs. Our experience points to special challenges created by stereoscopic video of swarms. These include the expected technical difficulties of capturing usable images of mosquitoes in the field, and creating an automated tracking system to enable generation of large numbers of three dimensional tracks over many seconds of footage. Once the data are collected, visualization and application of appropriate statistical and analytic methods also are required. We discuss our recent progress on these problems, give an example of a statistical approach to quantify individual male movement in a swarm with some novel results, and suggest further studies incorporating experimental manipulation and three dimensional localization and tracking of individual mosquitoes in wild swarms. Copyright (C) International Atomic Energy Agency 2013. Published by Elsevier B.V. All rights reserved. C1 [Manoukis, Nicholas C.] USDA ARS, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. [Butail, Sachit] NYU, Polytech Inst, Brooklyn, NY 11201 USA. [Diallo, Moussa] Univ Bamako, Unit Mol Ecol, Malaria Res & Training Ctr, Bamako, Mali. [Ribeiro, Jose M. C.] NIAID, Lab Malaria & Vector Res, Bethesda, MD 20892 USA. [Paley, Derek A.] Univ Maryland, Dept Aerosp Engn, College Pk, MD 20742 USA. [Paley, Derek A.] Univ Maryland, Syst Res Inst, College Pk, MD 20742 USA. RP Manoukis, NC (reprint author), USDA ARS PBARC, 64 Nowelo St, Hilo, HI 96720 USA. EM nicholas.manoukis@ars.usda.gov; sb4304@nyu.edu; moussad@icermali.org; jribeiro@niaid.nih.gov; dpaley@umd.edu RI Butail, Sachit/O-2928-2013; OI Butail, Sachit/0000-0001-9785-7374; Manoukis, Nicholas/0000-0001-5062-7256; Ribeiro, Jose/0000-0002-9107-0818 FU NIH; NIAID; IAEA CRP [G34002] FX We would like to thank Adama Dao and our other collaborators at the Malaria Research and Training Center in Bamako, Mali for help capturing these data; Richard Sakai, Robert Gwadz and Sekou Traore provided essential support. We also acknowledge input from Tovi Lehmann and Abdouleye Diabate. Special thanks to the people of Doneguebougou for allowing us to work in their village and homes. This research was supported in part by the Intramural Research Program of the NIH, NIAID and by IAEA CRP G34002. NR 35 TC 5 Z9 5 U1 0 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD APR PY 2014 VL 132 SU S SI SI BP S80 EP S85 DI 10.1016/j.actatropica.2013.06.021 PG 6 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA AG0NK UT WOS:000335112000011 PM 23850507 ER PT J AU Lamb, JFS Jung, HJG Riday, H AF Lamb, Joann F. S. Jung, Hans-Joachim G. Riday, Heathcliffe TI Growth environment, harvest management and germplasm impacts on potential ethanol and crude protein yield in alfalfa SO BIOMASS & BIOENERGY LA English DT Article DE Medicago sativa L.; Alfalfa; Leaf crude protein; Theoretical ethanol yield; Lignin ID CELL-WALL COMPOSITION; FORAGE QUALITY; MULTIFOLIOLATE ALFALFA; GENETIC-VARIATION; STEMS; LEAF; POPULATION; MATURITY; DIGESTIBILITY; DEGRADABILITY AB An alfalfa (Medicago sativa L) biomass energy production system would produce two products. Leaves would be separated from stems to produce a protein feed for livestock while stems would be processed to produce ethanol. Therefore, maximum yields of both leaves and stems are essential for profitability of this biomass production system. Our objective was to evaluate the impact of growth environment (locations, years and plant density) and harvest maturity stage (early bud (4 annual cuts) and late flower (3 annual cuts)) on leaf crude protein and potential ethanol yields for four alfalfa germplasms, two with high forage quality, and two non-lodging biomass types. Potential ethanol yield was greater at late flower compared to early bud, while leaf crude protein concentration was similar at the two harvest maturity stages at both locations. Leaf crude protein yield was greater at the Minnesota (MN) site compared to Wisconsin (WI) site. The two non-lodging biomass germplasms had greater potential ethanol yield compared to the high forage quality cultivars in WI, but no differences among the alfalfa germplasms were found for ethanol yield at MN. In WI, no differences were found among the germplasms for leaf crude protein yield, but the high quality cultivars had greater leaf crude protein yield than the non-lodging germpLasms in MN. While germplasm differences were found for leaf crude protein and potential ethanol yields, the environmental influences of harvest date and locations had the greatest impact on these two alfalfa biomass energy products. Published by Elsevier Ltd. C1 [Lamb, Joann F. S.] ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. [Lamb, Joann F. S.; Jung, Hans-Joachim G.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Riday, Heathcliffe] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Lamb, JFS (reprint author), ARS, USDA, Plant Sci Res Unit, 411 Borlaug Hall,1991 Upper Buford Circle, St Paul, MN 55108 USA. EM JoAnn.Lamb@ars.usda.gov NR 40 TC 5 Z9 5 U1 1 U2 15 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD APR PY 2014 VL 63 BP 114 EP 125 DI 10.1016/j.biombioe.2014.02.006 PG 12 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AG2VP UT WOS:000335275500014 ER PT J AU Wang, CX El-Shetehy, M Shine, MB Yu, KS Navarre, D Wendehenne, D Kachroo, A Kachroo, P AF Wang, Caixia El-Shetehy, Mohamed Shine, M. B. Yu, Keshun Navarre, Duroy Wendehenne, David Kachroo, Aardra Kachroo, Pradeep TI Free Radicals Mediate Systemic Acquired Resistance SO CELL REPORTS LA English DT Article ID NITRIC-OXIDE; PLANT IMMUNITY; ARABIDOPSIS-THALIANA; S-NITROSOGLUTATHIONE; CUTICLE FORMATION; SALICYLIC-ACID; AZELAIC-ACID; CELL-DEATH; RESPONSES; DEFENSE AB Systemic acquired resistance (SAR) is a form of resistance that protects plants against a broad spectrum of secondary infections. However, exploiting SAR for the protection of agriculturally important plants warrants a thorough investigation of the mutual interrelationships among the various signals that mediate SAR. Here, we show that nitric oxide (NO) and reactive oxygen species (ROS) serve as inducers of SAR in a concentration-dependent manner. Thus, genetic mutations that either inhibit NO/ROS production or increase NO accumulation (e.g., a mutation in S-nitrosoglutathione reductase [GSNOR]) abrogate SAR. Different ROS function additively to generate the fatty-acid-derived azelaic acid (AzA), which in turn induces production of the SAR inducer glycerol-3-phosphate (G3P). Notably, this NO/ROS -> AzA -> G3P-induced signaling functions in parallel with salicylic acid-derived signaling. We propose that the parallel operation of NO/ROS and SA pathways facilitates coordinated regulation in order to ensure optimal induction of SAR. C1 [Wang, Caixia] Qingdao Agr Univ, Coll Agron & Plant Protect, Qingdao 266109, Peoples R China. [Wang, Caixia; El-Shetehy, Mohamed; Shine, M. B.; Yu, Keshun; Kachroo, Aardra; Kachroo, Pradeep] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Navarre, Duroy] Washington State Univ, USDA ARS, Prosser, WA 99350 USA. [Wendehenne, David] Univ Bourgogne, ERL CNRS 6300, UMR Agroecol 1347, F-21065 Dijon, France. RP Kachroo, P (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM pk62@uky.edu OI Wendehenne, David/0000-0002-1088-102X FU National Science Foundation [MCB-0421914, IOS-051909]; United Soybean Board [1244]; Egyptian government FX This work was supported by grants from the National Science Foundation (MCB-0421914 and IOS-051909) and the United Soybean Board (1244). M.E.-S. was supported by a fellowship from the Egyptian government. We thank Joanne Holden for help with SA estimations, Guillaume Robin for help with images, Gah-Hyun Lim for help with NO estimations, Evan Stearns for help with expression analysis, Anne-Frances Miller for help with EPR analysis, and Ludmila Lapchyk and Amy Crume for technical support. We thank Nigel Crawford for noa1, Dan Klessig for bsmt1, Zhen-Ming Pei for nox1, Miguel Angel Torres and Jeff Dangl for rbohD and rbohF, and the Arabidopsis Biological Resource Center for nia1, nia2, and gsnor1 seeds. NR 34 TC 28 Z9 30 U1 3 U2 29 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 2211-1247 J9 CELL REP JI Cell Reports PD APR PY 2014 VL 7 IS 2 BP 348 EP 355 DI 10.1016/j.celrep.2014.03.032 PG 8 WC Cell Biology SC Cell Biology GA AG5EO UT WOS:000335442300006 PM 24726369 ER PT J AU Wang, S Matthan, NR Wu, DY Reed, DB Bapat, P Yin, XL Grammas, P Shen, CL Lichtenstein, AH AF Wang, Shu Matthan, Nirupa R. Wu, Dayong Reed, Debra B. Bapat, Priyanka Yin, Xiangling Grammas, Paula Shen, Chwan-Li Lichtenstein, Alice H. TI Lipid content in hepatic and gonadal adipose tissue parallel aortic cholesterol accumulation in Mice fed diets with different omega-6 PUFA to EPA plus DHA ratios SO CLINICAL NUTRITION LA English DT Article DE Atherosclerosis; Liver; Gonadal adipose tissue; Fatty acids; Lipid metabolism; Omega-3 fatty acids ID FATTY LIVER-DISEASE; CARDIOVASCULAR-DISEASE; INSULIN-RESISTANCE; METABOLIC SYNDROME; FISH-OIL; ACID; RISK; ATHEROSCLEROSIS; MACROPHAGES; OBESITY AB Background & aims: Diets with low omega (omega)-6 polyunsaturated fatty acids (PUFA) to eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) ratios have been shown to decrease aortic cholesterol accumulation and have been suggested to promote weight loss. The involvement of the liver and gonadal adipose tissue (GAT) in mediating these effects is not well understood. LDL receptor null mice were used to assess the effect of an atherogenic diet with different omega-6:EPA+DHA ratios on weight gain, hepatic and GAT lipid accumulation, and their relationship to atherosclerosis. Methods: Four groups of mice were fed a high saturated fat and cholesterol diet (HSF omega-6) alone, or with omega-6 PUFA to EPA+DHA ratios up to 1:1 for 32 weeks. Liver and GAT were collected for lipid and gene expression analysis. Results: The fatty acid profile of liver and GAT reflected the diets. All diets resulted in similar weight gains. Compared to HSF omega-6 diet, the 1:1 ratio diet resulted in lower hepatic total cholesterol (TC) content. Aortic TC was positively correlated with hepatic and GAT TC and triglyceride. These differences were accompanied by significantly lower expression of CD36, ATP-transporter cassette A1, scavenger receptor B class 1, 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), acetyl-CoA carboxylase alpha, acyl-CoA synthetase long-chain family member 5, and stearoyl-coenzyme A desaturase 1 (SCD1) in GAT, and HMGCR, SCD1 and cytochrome P450 7A1 in liver. Conclusions: Dietary omega-6:EPA+DHA ratios did not affect body weight, but lower omega-6:EPA+DHA ratio diets decreased liver lipid accumulation, which possibly contributed to the lower aortic cholesterol accumulation. (C) 2013 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. C1 [Wang, Shu; Reed, Debra B.; Bapat, Priyanka] Texas Tech Univ, Dept Nutr, Lubbock, TX 79409 USA. [Shen, Chwan-Li] Texas Tech Univ, Hlth Sci Ctr, Dept Pathol, Lubbock, TX 79430 USA. [Matthan, Nirupa R.; Wu, Dayong; Lichtenstein, Alice H.] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Yin, Xiangling; Grammas, Paula] Texas Tech Univ, Hlth Sci Ctr, Garrison Inst Aging, Lubbock, TX 79430 USA. RP Wang, S (reprint author), Texas Tech Univ, Dept Nutr, Box 41240, Lubbock, TX 79409 USA. EM shu.wang@ttu.edu FU Texas Tech; National Heart, Lung and Blood Institute [T32 HL69772-01A1] FX The study was supported by the Texas Tech Startup Funding to Shu Wang and the National Heart, Lung and Blood Institute grant T32 HL69772-01A1 (Shu Wang). NR 30 TC 4 Z9 4 U1 0 U2 14 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0261-5614 EI 1532-1983 J9 CLIN NUTR JI Clin. Nutr. PD APR PY 2014 VL 33 IS 2 BP 260 EP 266 DI 10.1016/j.clnu.2013.04.009 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8SI UT WOS:000334985500012 PM 23672804 ER PT J AU Stoy, ACF Heegaard, PMH Thymann, T Bjerre, M Skoygaard, K Boye, M Stoll, B Schmidt, M Jensen, BB Sangild, PT AF Stoy, Ann Cathrine F. Heegaard, Peter M. H. Thymann, Thomas Bjerre, Mette Skoygaard, Kerstin Boye, Mette Stoll, Barbara Schmidt, Mette Jensen, Bent B. Sangild, Per T. TI Bovine colostrum improves intestinal function following formula-induced gut inflammation in preterm pigs SO CLINICAL NUTRITION LA English DT Article DE Bovine colostrum; Necrotizing enterocolitis; Inflammation; Animal model ID NECROTIZING ENTEROCOLITIS; PARENTERAL-NUTRITION; ENTERAL NUTRITION; NEONATAL PIGLETS; DIET; COLONIZATION; DYSFUNCTION; RESPONSES; PROTEIN; MILK AB Background & aims: Only few hours of formula feeding may induce proinflammatory responses and predispose to necrotizing enterocolitis (NEC) in preterm pigs. We hypothesized that bovine colostrum, rich in bioactive factors, would improve intestinal function in preterm pigs following an initial exposure to formula feeding after some days of total parenteral nutrition (TPN). Methods: After receiving TPN for 2 days, preterm pigs were fed formula (FORM, n = 14), bovine colostrum (COLOS, n = 6), or formula (6 h) followed by bovine colostrum (FCOLOS, n = 14). Intestinal lesions, function, and structure, abundance and location of bacteria, and inflammation markers were investigated. Results: NEC severity and interleukins (1)-1 beta and -8 protein concentrations were lower, while villus height, galactose absorption, and brush-border enzyme activities were increased in the distal small intestine in COLOS and FCOLOS pigs, relative to FORM pigs. Intestinal gene expression of serum amyloid A, IL-1 beta, -6 and -8, and bacterial abundance, correlated positively with NEC severity of the distal small intestine. Conclusions: Bovine colostrum restores intestinal function after initial formula-induced inflammation in preterm pigs. Further studies are required to test if bovine colostrum may also benefit preterm infants during the challenging transition from total parenteral nutrition to,enteral nutrition, when human milk is unavailable. (C) 2013 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved. C1 [Stoy, Ann Cathrine F.; Heegaard, Peter M. H.; Skoygaard, Kerstin; Boye, Mette] Tech Univ Denmark, Natl Vet Inst, DK-1870 Frederiksberg C, Denmark. [Thymann, Thomas; Sangild, Per T.] Univ Copenhagen, Dept Nutr Exercise & Sports, DK-1958 Frederiksberg C, Denmark. [Bjerre, Mette] Aarhus Univ, Med Res Labs, Dept Clin Med, Fac Hlth Sci, Aarhus C, Denmark. [Stoll, Barbara] Baylor Coll Med, USDA, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Schmidt, Mette] Univ Copenhagen, Dept Large Anim Sci Vet Reprod & Obstet, DK-1958 Frederiksberg C, Denmark. [Jensen, Bent B.] Aarhus Univ, Dept Anim Sci, DK-8030 Tjele C, Denmark. RP Sangild, PT (reprint author), Univ Copenhagen, Dept Nutr Exercise & Sports, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark. EM acfst@vet.dtu.dk; pmhh@vet.dtu.dk; ttn@life.ku.dk; mette.bjerre@ki.au.dk; kesk@vet.dtu.dk; mboy@vet.dtu.dk; bstoll@bcm.tmc.edu; mhs@life.ku.dk; bentborg.jensen@agrsci.dk; psa@life.ku.dk RI Navntoft, Anne Mette/G-8527-2013; OI skovgaard, kerstin/0000-0001-5663-4879; Sangild, Per Torp/0000-0002-5462-7760 FU Danish Council for Strategic Research; Biofiber-Damino FX The study was funded by The Danish Council for Strategic Research and by Biofiber-Damino (donation of colostrum). NR 30 TC 19 Z9 19 U1 2 U2 27 PU CHURCHILL LIVINGSTONE PI EDINBURGH PA JOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLAND SN 0261-5614 EI 1532-1983 J9 CLIN NUTR JI Clin. Nutr. PD APR PY 2014 VL 33 IS 2 BP 322 EP 329 DI 10.1016/j.clnu.2013.05.013 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AF8SI UT WOS:000334985500022 PM 23791159 ER PT J AU Pechal, JL Benbow, ME Crippen, TL Tarone, AM Tomberlin, JK AF Pechal, Jennifer L. Benbow, M. Eric Crippen, Tawni L. Tarone, Aaron M. Tomberlin, Jeffery K. TI Delayed insect access alters carrion decomposition and necrophagous insect community assembly SO ECOSPHERE LA English DT Article DE carrion; community assembly; decomposition ecology; ephemeral resource; necrobiome; necrophagous insects ID ACCUMULATED DEGREE-DAYS; TERRESTRIAL ECOSYSTEMS; LITTER DECOMPOSITION; BLOW FLIES; BIODIVERSITY; DYNAMICS; SUCCESSION; DIVERSITY; REMAINS; COEXISTENCE AB Vertebrate carrion in terrestrial ecosystems is an unpredictable, ephemeral resource pulse that contributes to local biodiversity and nutrient transformation dynamics. Carrion ecology is infrequently studied compared to other decomposition systems, such as leaf litter detritus, despite its importance as a resource subsidy in most ecosystems. We hypothesized that delayed insect access to carrion ( insects excluded for five days) would demonstrate marked shifts in necrophagous insect community structure, turnover rates and assembly with overall effects on carrion decomposition. Despite similarities between taxon arrival patterns, once insects were allowed to colonize carrion previously excluded from insects there was an increased necrophagous insect taxon richness and increased community turnover rates. Additionally, during the first five days of decomposition, insect exclusion carcasses remained in bloat stage while those naturally colonized were well advanced in active decomposition. This resulted in substantial differences in decomposition and highlighted the importance of insect community assembly in the decomposition process. Carrion decomposition has been a neglected field of study compared to other organic matter processes (e.g., leaf detritus), and these data suggest the ecology of carrion-arthropod interactions can contribute to a broader understanding of decomposition processes and ecosystem function. C1 [Pechal, Jennifer L.; Tarone, Aaron M.; Tomberlin, Jeffery K.] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA. [Benbow, M. Eric] Univ Dayton, Dept Biol, Dayton, OH 45469 USA. [Crippen, Tawni L.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Pechal, JL (reprint author), Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. EM pechalje@msu.edu OI Tarone, Aaron/0000-0003-0965-7634 FU University of Dayton Research Council; Department of Biology FX The authors thank A. Lewis, T. Blair, and J. White for help during the study. The Blair family is gratefully acknowledged for allowing access to their property for this research. This work was done in partial fulfillment of the dissertation produced by J. L. Pechal. J. L. Pechal and J. K. Tomberlin would like to thank the Department of Entomology at Texas A&M University, Texas A&M University AgriLife Research and the Whole Systems Genome Initiative at Texas A&M University. M. E. Benbow was supported by the University of Dayton Research Council and the Department of Biology. NR 63 TC 7 Z9 7 U1 7 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD APR PY 2014 VL 5 IS 4 AR 45 DI 10.1890/ES14-00022.1 PG 21 WC Ecology SC Environmental Sciences & Ecology GA AF9BT UT WOS:000335010800008 ER PT J AU Parresol, BR Edwards, LA AF Parresol, Bernard R. Edwards, Lloyd A. TI An Entropy-Based Contagion Index and Its Sampling Properties for Landscape Analysis SO ENTROPY LA English DT Article DE concentration; delta method; expected value; forest types; geometric distribution; hypothesis testing; simulated landscapes ID DIVERSITY; PATTERNS AB Studies of spatial patterns of landscapes are useful to quantify human impact, predict wildlife effects, or describe various landscape features. A robust landscape index should quantify two components of landscape diversity: composition and configuration. One category of landscape index is the contagion index. Some landscape ecologists promote the use of relative contagion indices. It is demonstrated, using simulated landscapes, that relativized contagion indices are mathematically untenable. A new entropy contagion index (G) is developed. Distributional properties of (Gamma) over cap are derived. It is shown to be asymptotically unbiased, consistent, and asymptotically normally distributed. A variance formula for (Gamma) over cap is derived using the delta method. As an application, the pattern and changes in forest types across four soil-geologic landform strata were analyzed on the 80,000 ha Savannah River Site in South Carolina, USA. One-way analysis of variance was used for hypothesis testing of contagion among strata. The differences in contagion across the strata provide insight to managers to meet structural objectives. C1 [Parresol, Bernard R.; Edwards, Lloyd A.] US Forest Serv, USDA, Southern Res Stn, Asheville, NC 28806 USA. RP Parresol, BR (reprint author), US Forest Serv, USDA, Southern Res Stn, Asheville, NC 28806 USA. EM bparresol@fs.fed.us; laedwards@fs.fed.us FU USDA Forest Service Southern Research Station; Department of Energy-Savannah River Operations Office through the U.S. Forest Service Savannah River [DE-AI09-00SR22188] FX We would like to thank Harbin Li for use of his landscape simulation program, SHAPC. We would also like to thank Lisa Parresol for her graphic of Figure 1. Funding was provided by the USDA Forest Service Southern Research Station and the Department of Energy-Savannah River Operations Office through the U.S. Forest Service Savannah River under interagency Agreement DE-AI09-00SR22188. NR 33 TC 1 Z9 1 U1 2 U2 8 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 1099-4300 J9 ENTROPY-SWITZ JI Entropy PD APR PY 2014 VL 16 IS 4 BP 1842 EP 1859 DI 10.3390/e16041842 PG 18 WC Physics, Multidisciplinary SC Physics GA AF8YI UT WOS:000335001500001 ER PT J AU Masi, M Meyer, S Cimmino, A Andolfi, A Evidente, A AF Masi, Marco Meyer, Susan Cimmino, Alessi Andolfi, Anna Evidente, Antonio TI Pyrenophoric Acid, a Phytotoxic Sesquiterpenoid Penta-2,4-dienoic Acid Produced by a Potential Mycoherbicide, Pyrenophora semeniperda SO JOURNAL OF NATURAL PRODUCTS LA English DT Article ID ABSCISIC-ACID; BIOSYNTHESIS; PLANTS AB A new phytotoxic sesquiterpenoid penta-2,4-dienoic acid, named pyrenophoric acid, was isolated from solid wheat seed culture of Pyrenophora semeniperda, a fungal pathogen proposed as a mycoherbicide for biocontrol of cheatgrass (Bromus tectorum) and other annual bromes. These 1 bromes are serious weeds in winter cereals and also on temperate semiarid rangelands. Pyrenophoric acid was characterized as (2Z,4E)-5-[(7S,9S,10R,12R)-3,4-dihydroxy-2,2,6-trimethylcyclohexyl)]-3-methylpenta-2,4-dienoic acid by spectroscopic and chemical methods. The relative stereochemistry of pyrenophoric acid was assigned using H-1,H-1 couplings and NOESY experiments, while its absolute configuration was determined by applying the advanced Mosher's method. Pyrenophoric acid is structurally quite closely related to the plant growth regulator abscisic acid. When bioassayed in a cheatgrass coleoptile elongation test at 10(-3) M, pyrenophoric acid showed strong phytotoxicity, reducing coleoptile elongation by 51% relative to the control. In a mixture at 10(-4) M, its negative effect on coleoptile elongation was additive with that of cytochalasin B, another phytotoxic compound found in the wheat seed culture extract of this fungus, demonstrating that the extract toxicity observed in earlier studies was due to the combined action of multiple phytotoxic compounds. C1 [Masi, Marco; Cimmino, Alessi; Andolfi, Anna; Evidente, Antonio] Univ Naples Federico II, Dipartimento Sci Chim, I-80126 Naples, Italy. [Masi, Marco] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84601 USA. [Meyer, Susan] US Forest Serv, Rocky Mt Res Stn, Shrub Sci Lab, Provo, UT 84606 USA. RP Evidente, A (reprint author), Univ Naples Federico II, Dipartimento Sci Chim, Complesso Univ Monte St Angelo,Via Cintia 4, I-80126 Naples, Italy. EM evidente@unina.it RI Evidente, Antonio/N-9357-2013; Masi, Marco/J-7742-2016; Cimmino, Alessio/I-2121-2012 OI Evidente, Antonio/0000-0001-9110-1656; Masi, Marco/0000-0003-0609-8902; Cimmino, Alessio/0000-0002-1551-4237 FU Joint Fire Sciences Program of the U.S. Departments of Agriculture and Interior [JESP-11-S-2-6] FX Some of the NMR spectra were recorded in the laboratory of the Instituto di Chimica Biomolecolare del CNR, Pozzuoli, Italy, by Mrs. D. Melck, whose contribution is gratefully acknowledged. Logistical support at Brigham Young University was provided to M.M. by Dr. P. Savage of the Chemistry Department. We also gratefully acknowledge S. Clement of the USFS Shrub Sciences Laboratory, who produced the fungal cultures, F. Avolio of the University of Naples Federico II Department of Chemistry, for useful discussion, and S. Mata, who assisted with the spectroscopic analyses carried out at Brigham Young University. This research was funded in part by Grant JESP-11-S-2-6 to S.E.M. from the Joint Fire Sciences Program of the U.S. Departments of Agriculture and Interior. NR 28 TC 9 Z9 9 U1 1 U2 16 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0163-3864 EI 1520-6025 J9 J NAT PROD JI J. Nat. Prod. PD APR PY 2014 VL 77 IS 4 BP 925 EP 930 DI 10.1021/np4009915 PG 6 WC Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy GA AG0SZ UT WOS:000335127200026 PM 24641210 ER PT J AU Tucker, AM Campbell, JF Arthur, FH Zhu, KY AF Tucker, Angela M. Campbell, James F. Arthur, Frank H. Zhu, Kun Yan TI Mechanisms for horizontal transfer of methoprene from treated to untreated Tribolium castaneum (Herbst) SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Triloolium castaneum; Horizontal transfer; Methoprene; Insecticide; Aerosol ID PROLONGS DEVELOPMENTAL TIME; STORED-PRODUCT COLEOPTERANS; INDIANMEAL MOTH LEPIDOPTERA; INCREASES MORTALITY; FLOUR BEETLES; HYDROPRENE; POPULATIONS; FUMIGATION; EFFICACY; PATTERNS AB Experiments were performed to determine the relative impact of different mechanisms of methoprene horizontal transfer between Tribolium castaneum (Herbst) individuals. Larvae provided during later development with only dead methoprene-treated developmental stages (late-stage larvae, pupae, or adults) resulted in no survival to adulthood. This suggests that transfer through cuticle contact could be involved, but some cannibalization also occurred. The average level of cannibalization was not different between methoprene and control treatments, but the distribution of the amount of feeding did appear to be shifted to greater consumption by larvae exposed to methoprene-treated individuals compared to water-treated. The potential impact of cannibalism on horizontal transfer was further evaluated by scoring the level of feeding by untreated larvae on either water or methoprene-treated pupa and then comparing larval development and survival after different levels of cannibalization. In this experiment, the level of feeding on methoprene-treated individuals did not affect the percentage of normal surviving adults (insects which emerged as adults, exhibited no external morphological malformations, and were alive at the end of the observational period), suggesting that consumption of treated individuals was not contributing significantly to horizontal transfer. Short-term confinement of a methoprene or water treated adult with an untreated late-stage larva did not effect larval development and survival compared to the controls, indicating that brief contact between individuals was not sufficient for detectable negative effects. Transfer of methoprene from treated individuals to a flour substrate and then to an untreated individual through contact with and/or feeding on the flour was evaluated. Exposure of larvae to methoprene-contaminated flour during development resulted in a significantly lower number of normal surviving adults compared to controls. These results suggest that horizontal transfer of methoprene is probably due to prolonged contact with methoprene-treated individuals or methoprene-contaminated flour. Published by Elsevier Ltd. C1 [Tucker, Angela M.; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Campbell, James F.; Arthur, Frank H.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Campbell, JF (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov FU U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) [2010-51102-21660]; Kansas Agricultural Experiment Station, Kansas State University FX We thank Doug VanGundy of Central Sciences International for supplying methoprene; Casey Persson, Marshall Van Tuyl, Brian Sanbourn, Rich Hammel, Chelle Hartzer, and Brian Barnett for technical support and advice; and Dr. Leigh Murray, Zhining Oh, Carlie Shannon, and Dr. Karrie Buckman for statistical advice. We, also, thank Dr. Subramanyam Bhadrirarju, Dr. Susan Brown, and Dr. David Margolies for comments on earlier drafts. This research was partially funded by the U.S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program (grant number 2010-51102-21660). This paper is contribution number 14-066-J of the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, KS. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture or Kansas State University. USDA and Kansas State University are equal opportunity providers and employers. NR 25 TC 1 Z9 1 U1 3 U2 7 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD APR PY 2014 VL 57 BP 36 EP 42 DI 10.1016/j.jspr.2014.02.004 PG 7 WC Entomology SC Entomology GA AG2ZI UT WOS:000335285200006 ER PT J AU Tucker, AM Campbell, JF Arthur, FH Zhu, KY AF Tucker, Angela M. Campbell, James F. Arthur, Frank H. Zhu, Kun Yan TI Horizontal transfer of methoprene by Tribolium castaneum (Herbst) and Tribolium confusum Jacquelin du Val SO JOURNAL OF STORED PRODUCTS RESEARCH LA English DT Article DE Tribolium castaneum; Tribolium confusum; Horizontal transfer; Methoprene; Aerosol applications ID PLODIA-INTERPUNCTELLA HUBNER; INSECT GROWTH-REGULATORS; FLOUR BEETLES; COLEOPTERA; TENEBRIONIDAE; POPULATIONS; HYDROPRENE; EFFICACY; MILLS; SUSCEPTIBILITY AB In food facilities the majority of insect populations typically occur within hidden locations with limited direct exposure to insecticides, but there is potential for dispersing insects to transport insecticides into hidden areas and transfer insecticide to other individuals (i.e., horizontal transfer). In a series of laboratory experiments, the potential for transfer of the insect growth regulator methoprene between treated and untreated Tribolium castaneum (Herbst) and Tribolium confiisum (Jacquelin du Val) individuals was evaluated. In the first experiment, late-instar larvae were exposed to methoprene or water treated individuals in flour and their ability to develop successfully was determined. For T. castaneum, survival time for individuals exposed to methoprene treated individuals was shorter than those exposed to control individuals, but the difference was not significant for T. confusum. For both T castaneum and T. confusum, the number of individuals that were able to successfully survive exposure and complete development without externally visible deformities was significantly reduced for individuals exposed to methoprene treated pupae or larvae relative to controls, but not when exposed to methoprene treated adults. In the second experiment, early-stage larvae, late-stage larvae, and pupae were similar in susceptibility to horizontal transfer of methoprene, even though they were exposed as immatures for different lengths of time. In a third experiment, the potential for sublethal effects due to horizontal transfer of methoprene was evaluated, but no difference in reproduction and oviposition was found for normal appearing adults that developed when exposed to methoprene treated or and control individuals. This is the first report indicating that methoprene can be transferred between stored-product insects and further research is needed to determine the impact this potentially has on pest populations. Published by Elsevier Ltd. C1 [Tucker, Angela M.; Zhu, Kun Yan] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Campbell, James F.; Arthur, Frank H.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Campbell, JF (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM james.campbell@ars.usda.gov FU U.S. Department of Agriculture; National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program [2010-51102-21660] FX We thank Doug VanGundy of Central Life Sciences for supplying methoprene; Rich Hammel, Chelle Hartzer, Casey Persson, Brian Barnett, and Marshal VanTuyl for technical support and advice; and Leigh Murray, Zhining Oh, Carlie Shannon, and Karrie Buckman for statistical advice. Research was partially funded by the U. S. Department of Agriculture, National Institute of Food and Agriculture (NIFA) Methyl Bromide Transitions program (grant number 2010-51102-21660). This manuscript is contribution no. 14-023-j from the Kansas Agricultural Experiment Station, Kansas State University, Manhattan, Kansas. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture or Kansas State University. USDA is an equal opportunity provider and employer. NR 39 TC 5 Z9 5 U1 0 U2 6 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-474X EI 1879-1212 J9 J STORED PROD RES JI J. Stored Prod. Res. PD APR PY 2014 VL 57 BP 73 EP 79 DI 10.1016/j.jspr.2013.12.001 PG 7 WC Entomology SC Entomology GA AG2ZI UT WOS:000335285200011 ER PT J AU Liu, X Byrd, JA Farnell, M Ruiz-Feria, CA AF Liu, X. Byrd, J. A. Farnell, M. Ruiz-Feria, C. A. TI Arginine and vitamin E improve the immune response after a Salmonella challenge in broiler chicks SO POULTRY SCIENCE LA English DT Article DE immune response; Salmonella; arginine; vitamin E; mannanoligosaccharide ID ESCHERICHIA-COLI CHALLENGE; NITRIC-OXIDE; TYPHIMURIUM COLONIZATION; MANNAN-OLIGOSACCHARIDES; NUTRITIONAL MODULATION; PROTECTIVE IMMUNITY; PURIFIED LIGNIN; ORGAN INVASION; T-CELLS; ENTERITIDIS AB Two experiments were conducted to evaluate the effects of Arg, vitamin E (VE), and mannanoligosaccharide ( MOS) on the immune response and clearance of Salmonella in broiler chickens. In each experiment, 1- d- old chicks (n = 160) were randomly distributed into 4 groups: antibiotic-free diet (negative control, CTL-), antibiotic-supplemented diet (positive control, CTL+), antibiotic free-diet plus Arg and VE (AVE), or antibiotic-free diet plus Arg, VE, and MOS (AVM). Birds were orally challenged with 10(6) cfu of a novobiocyn and nalidixic acid-resistant Salmonella enterica serovar Typhimurium strain at d 7 (experiment 1) or at d 3 (experiment 2). Heterophil-(HOB) and monocyte-(MOB) oxidative burst and lymphocyte proliferation (LPR), antibody titers, and Salmonella content in the ceca were measured at several intervals postinfection (PI). In experiment 1, both AVM and AVE decreased HOB compared with the controls 5 and 9 d PI, but increased LPR 9 d PI. In the same experiment, birds fed the AVE diet had higher MOB than birds fed CTL+ or the AVM diet at 7 d PI, whereas 9 d PI birds fed the AVM diet had the highest MOB. In experiment 2, birds fed the AVE diet had higher MOB, HOB, and LPR than birds in the other treatments 7 and 14 d PI, except at 7 d PI, when MOB was not different among treatments. Birds fed the AVM diet had the highest IgA antibody titer, and a higher IgM antibody titer than the CTL+ birds. In experiment 1, Salmonella Typhimurium content in the ceca was lower in birds fed the AVM diet compared with birds fed the CTL-diet 3 d PI, but later on (10 and 17 d PI), and in experiment 2 (7, 14, and 21 d PI), Salmonella Typhimurium concentrations were not different among treatments. Thus, Arg and VE improved immune response after a Salmonella Typhimurium challenge in young chicks, and although they did not reduce Salmonella Typhimurium concentrations in the ceca, they may improve bacterial resistance against other pathogens in commercial growing conditions. C1 [Liu, X.; Ruiz-Feria, C. A.] Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. [Byrd, J. A.] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Farnell, M.] Mississippi State Univ, Dept Poultry Sci, Mississippi State, MS 39762 USA. RP Ruiz-Feria, CA (reprint author), Texas A&M Univ, Dept Poultry Sci, College Stn, TX 77843 USA. EM ciroruizferia@gmail.com NR 56 TC 5 Z9 6 U1 2 U2 26 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD APR PY 2014 VL 93 IS 4 BP 882 EP 890 DI 10.3382/ps.2013-03723 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AE9SL UT WOS:000334349500013 PM 24706965 ER PT J AU Olanrewaju, HA Purswell, JL Collier, SD Branton, SL AF Olanrewaju, H. A. Purswell, J. L. Collier, S. D. Branton, S. L. TI Effects of genetic strain and light intensity on blood physiological variables of broilers grown to heavy weights(1) SO POULTRY SCIENCE LA English DT Article DE genetic strain; light intensity; acid-base balance; broiler; well-being ID ACID-BASE-BALANCE; CHICKENS; ASCITES; OXYGEN; ABNORMALITIES; TEMPERATURE; PERFORMANCE; DISORDERS; POULTRY; STRESS AB The effects of genetic strain, light intensity, and their interaction were examined on blood physiological variables of broilers maintained in environmentally controlled rooms in each of 5 trials. The study consisted of a 2 x 5 factorial arranged in a randomized complete block design with 10 treatments of 2 strains (Ross x Ross 308 and 708) and exposure to 5 light intensities (25, 10, 5, 2.5, and 0.2 lx) with chicks equally and randomly distributed at 1 d of age. The 5 light intensities were randomly assigned from 22 to 56 d of age. Feed and water were provided ad libitum. Venous blood samples were collected on d 21 (base line), 28, 42, and 56 of age. Main effects indicate that Ross x Ross 308 significantly (P <= 0.05) increased BW, partial pressure of CO2, Ca2+, Na+, Cl-, and thyroxine, along with significantly (P <= 0.05) reduced pH level, partial pressure of O2, HCO3 (-), and triiodothyronine in comparison with Ross x Ross 708. No main effect of light intensity was observed on all examined variables. In addition, no significant main effects were observed for strain, light intensity, or their interaction on saturated O2, hematocrit, hemoglobin, K+, mean corpuscular hemoglobin concentration, glucose, osmolality, and anion gap. Physiologically, although Ross x Ross 308 had a significantly increased BW in comparison with Ross x Ross 708, Ross x Ross 708 showed a significant (P <= 0.05) increase in partial pressure of O-2 and triiodothyronine along with lower blood partial pressure of CO2 and thyroxine. The results of this study supplement current knowledge of physiological response to differing lighting levels and may be used to establish the normal blood values for commercial broilers grown to heavy weights. Plasma corticosterone was not affected by either treatments or strain, indicating that the treatments did not induce physiological stress in broilers. C1 [Olanrewaju, H. A.; Purswell, J. L.; Collier, S. D.; Branton, S. L.] USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. RP Olanrewaju, HA (reprint author), USDA ARS, Poultry Res Unit, POB 5367, Mississippi State, MS 39762 USA. EM Hammed.Olanrewaju@ars.usda.gov NR 37 TC 3 Z9 3 U1 4 U2 18 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD APR PY 2014 VL 93 IS 4 BP 970 EP 978 DI 10.3382/ps.2013-03613 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AE9SL UT WOS:000334349500023 PM 24706975 ER PT J AU Del Coco, VF Cordoba, MA Bilbao, G Castro, APD Basualdo, JA Fayer, R Santin, M AF Del Coco, Valeria F. Cordoba, Maria A. Bilbao, Gladys de Almeida Castro, Aldana Pinto Basualdo, Juan A. Fayer, Ronald Santin, Monica TI Cryptosporidium parvum GP60 subtypes in dairy cattle from Buenos Aires, Argentina SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE Argentina; Cattle; Cryptosporidium parvum; GP60; Molecular epidemiology; Subtypes ID MOLECULAR CHARACTERIZATION; GENETIC-CHARACTERIZATION; UNITED-STATES; CALVES; HUMANS; SPP.; POPULATION; PREVALENCE; INFECTION; GENOTYPE AB Cryptosporidium parvum from 73 dairy calves less than two months old from Buenos Aires province (Argentina) were molecularly characterized using sequence analysis of the GP60 gene. Seventy-five sequences were obtained, and seven different subtypes were identified, all belonging to the Ha subtype family. The most common subtypes were IIaA20G1R1 (27/75), IIaA22G1R1 (16/75), and IIaA18G1R1 (13/ 75). Subtypes IIaA21G1R1, IIaA23G1R1, IIaA16G1R1 and IIaA19G1R1 were found sporadically. Two samples contained mixed infections with IIaA21G1R1 and IIaA22G1R1. A significant association was found between subtypes and geographic location, whereas there was no relation between subtypes and presence of diarrhea. Three of the subtypes found in this study (IIaA16G1R1, IIaA18G1R1, and IIaA19G1R1) were previously identified in humans. These findings suggest that cattle could play an important role in the transmission of cryptosporidiosis to humans in Buenos Aires province. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Del Coco, Valeria F.; Cordoba, Maria A.; Basualdo, Juan A.] Univ Nacl La Plata, Fac Ciencias Med, Catedra Microbiol & Parasitol, RA-1900 La Plata, Buenos Aires, Argentina. [Del Coco, Valeria F.] Consejo Nacl Invest Cient & Tecn, RA-1033 Buenos Aires, DF, Argentina. [Cordoba, Maria A.] Comis Invest Cient Prov Buenos Aires, Buenos Aires, DF, Argentina. [Bilbao, Gladys; de Almeida Castro, Aldana Pinto] Univ Nacl Ctr Prov Buenos Aires, Fac Ciencias Vet, Buenos Aires, DF, Argentina. [Fayer, Ronald; Santin, Monica] ARS, Environm Microbial & Food Safety Lab, USDA, BARC East, Beltsville, MD 20705 USA. RP Del Coco, VF (reprint author), Univ Nacl La Plata, Fac Ciencias Med, Catedra Microbiol & Parasitol, 60 & 120, RA-1900 La Plata, Buenos Aires, Argentina. EM vdelcoco@aetos.med.unlp.edu.ar OI Basualdo, Juan/0000-0001-9126-3719 FU USDA; Universidad Nacional de La Plata FX The authors would like to thank Natalia Macarisin for technical assistance. This work was supported by USDA and Universidad Nacional de La Plata. NR 35 TC 5 Z9 5 U1 2 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD APR PY 2014 VL 96 IS 2 BP 311 EP 314 DI 10.1016/j.rvsc.2013.12.010 PG 4 WC Veterinary Sciences SC Veterinary Sciences GA AF8UD UT WOS:000334990200015 PM 24480390 ER PT J AU Stenfeldt, C Pacheco, JM Rodriguez, LL Arzt, J AF Stenfeldt, C. Pacheco, J. M. Rodriguez, L. L. Arzt, J. TI Infection dynamics of foot-and-mouth disease virus in pigs using two novel simulated-natural inoculation methods SO RESEARCH IN VETERINARY SCIENCE LA English DT Article DE FMDV; Virus; Pathogenesis; Animal models ID BETWEEN-PEN TRANSMISSION; VACCINATED PIGS; EARLY PATHOGENESIS; SWINE; ANTIBODY; CATTLE; IDENTIFICATION; AEROSOLS; CONTACT; SITES AB In order to characterize foot-and-mouth disease virus (FMDV) infection dynamics in pigs, two simulatednatural inoculation systems were developed and evaluated. Intra-oropharyngeal (lOP) and intra-nasopharyngeal (INP) inoculation both enabled precise control of dose and timing of inoculation while simulating field exposure conditions. There were substantial differences between outcomes of infections by the two routes. IOP inoculation resulted in consistent and synchronous infection, whereas INP inoculation at similar doses resulted in delayed, or completely absent infection. All pigs that developed clinical infection had detectable levels of FMDV RNA in their oropharynx directly following inoculation. Furthermore, FMDV antigens were localized to the oropharyngeal tonsils suggesting a role in early infection. The utility of LOP inoculation was further demonstrated in a vaccine-challenge experiment. Thus, the novel system of lOP inoculation described herein, offers a valid alternative to traditionally used systems for FMDV inoculation of pigs, applicable for experimental studies of FMDV pathogenesis and vaccinology. Published by Elsevier Ltd. C1 [Stenfeldt, C.; Pacheco, J. M.; Rodriguez, L. L.; Arzt, J.] Agr Res Serv, Plum Isl Anim Dis Ctr, Foreign Anim Dis Res Unit, USDA, Greenport, NY 11944 USA. [Stenfeldt, C.] Oak Ridge Inst Sci & Educ, PIADC Res Participat Program, Oak Ridge, TN 37831 USA. RP Arzt, J (reprint author), USDA ARS, Foreign Anim Dis Res Unit, Plum Isl Anim Dis Ctr, POB 848, Greenport, NY 11944 USA. EM Jonathan.Arzt@ars.usda.gov OI Stenfeldt, Carolina/0000-0002-2074-3886; Pacheco, Juan/0000-0001-5477-0201; Arzt, Jonathan/0000-0002-7517-7893 FU Science and Technology Directorate of the U.S. Department of Homeland Security [HSHQDC-11X-00189] FX This project was funded through an interagency agreement with the Science and Technology Directorate of the U.S. Department of Homeland Security under Award Number HSHQDC-11X-00189. CS is a recipient of a Plum Island Animal Disease Center Research Participation Program fellowship, administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement with the US Department of Energy. None of the funding sources had influence upon design or performance of experimental study, interpretation of results or writing of the manuscript. We would like to thank the Animal Research Branch at PIADC for assistance and support during animal studies. Meghan Tucker, Elizabeth Bishop, George Smoliga, and Ethan Hartwig are thanked for support with necropsies and processing of samples. NR 37 TC 8 Z9 8 U1 0 U2 4 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0034-5288 EI 1532-2661 J9 RES VET SCI JI Res. Vet. Sci. PD APR PY 2014 VL 96 IS 2 BP 396 EP 405 DI 10.1016/j.rvsc.2014.01.009 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA AF8UD UT WOS:000334990200027 PM 24548596 ER PT J AU Dogramaci, M Anderson, JV Chao, WS Foley, ME AF Dogramaci, Muenevver Anderson, James V. Chao, Wun S. Foley, Michael E. TI Foliar Application of Glyphosate Affects Molecular Mechanisms in Underground Adventitious Buds of Leafy Spurge (Euphorbia esula) and Alters Their Vegetative Growth Patterns SO WEED SCIENCE LA English DT Article DE Auxin transporters; endoplasmic reticulum; hormones; stunted and bushy phenotype; transcript abundance ID HORSEWEED CONYZA-CANADENSIS; CROWN BUDS; 5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; GIBBERELLIN METABOLISM; 3-PHOSPHATE SYNTHASE; HERBICIDE RESISTANCE; FLOWERING COMPETENCE; TRANSCRIPT PROFILES; SHIKIMATE PATHWAY; DORMANCY STATUS AB Long-term control of leafy spurge with glyphosate requires multiple applications because the plant reproduces vegetatively from abundant underground adventitious buds, referred to as crown and root buds. Determining the molecular mechanisms involved in controlling vegetative reproduction in leafy spurge following foliar glyphosate treatment could identify limiting factors or new targets for manipulation of plant growth and development in invasive perennial species. Thus, we treated leafy spurge plants with 0 or 2.24 kg ai ha(-1) glyphosate to determine its impact on selected molecular processes in crown buds derived from intact plants and plants decapitated at the soil surface 7 d after glyphosate treatment. New shoot growth from crown buds of foliar glyphosate-treated plants was significantly reduced compared with controls after growth-inducing decapitation, and had a stunted or bushy phenotype. Quantification of a selected set of transcripts involved in hormone biosynthesis and signaling pathways indicated that glyphosate had the most significant impact on abundance of ENT-COPALYL DIPHOSPHATE SYNTHETASE 1, which is involved in a committed step for gibberellin biosynthesis, and auxin transporters including PINs, PIN-LIKES, and ABC TRANSPORTERS. Foliar glyphosate treatment also reduced the abundance of transcripts involved in cell cycle processes, which would be consistent with altered growth patterns observed in this study. Overall, these results suggest that interplay among phytohormones such as auxin, ethylene, and gibberellins affect vegetative growth patterns from crown buds of leafy spurge in response to foliar glyphosate treatment. C1 [Dogramaci, Muenevver; Anderson, James V.; Chao, Wun S.; Foley, Michael E.] USDA ARS, Biosci Res Lab, Sunflower & Plant Biol Res Unit, Fargo, ND 58102 USA. RP Dogramaci, M (reprint author), USDA ARS, Biosci Res Lab, Sunflower & Plant Biol Res Unit, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM munevver.dogramaci@ars.usda.gov OI Anderson, James/0000-0002-1801-5767 NR 57 TC 3 Z9 3 U1 4 U2 24 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2014 VL 62 IS 2 BP 217 EP 229 DI 10.1614/WS-D-13-00156.1 PG 13 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AG4BL UT WOS:000335363600002 ER PT J AU Gealy, DR Anders, M Watkins, B Duke, S AF Gealy, David R. Anders, Merle Watkins, Bradley Duke, Sara TI Crop Performance and Weed Suppression by Weed-Suppressive Rice Cultivars in Furrow- and Flood-Irrigated Systems under Reduced Herbicide Inputs SO WEED SCIENCE LA English DT Article DE Allelopathy; bed-planted rice; acifluorfen; bentazon; clomazone; fenoxaprop; flood irrigation; furrow irrigation; glyphosate; halosulfuron; herbicides; propanil; quinclorac; weed control; weed suppression ID ECHINOCHLOA-CRUS-GALLI; WATER-SEEDED RICE; ISOTOPE DISCRIMINATION ANALYSIS; ORYZA-SATIVA; RELATIVE IMPORTANCE; SHOOT COMPETITION; ALLELOPATHIC RICE; INDICA RICE; ROOT COMPETITION; UPLAND RICE AB Weed control in rice is challenging, particularly in light of increased resistance to herbicides in weed populations and diminishing availability of irrigation water. Certain indica rice cultivars can produce high yields and suppress weeds in conventional flood-irrigated, drill-seeded systems in the southern United States under reduced herbicide inputs, but their response to reduced irrigation inputs in these systems in not known. Rice productivity and weed control by weed-suppressive cultivars and conventional nonsuppressive cultivars were evaluated in a nonflooded furrow-irrigated (FU) system and a conventionally flooded (FL) system under three levels of weed management (herbicide inputs) in a 3-yr field study. Rice yields across all weed management levels yielded similar to 76% less in the FU system than in the FL system. The allelopathic indica cultivar, 'PI 312777', and commercial hybrid rice 'CLXL729' generally produced the highest grain yields and greatest suppression of barnyardgrass in both irrigation systems. 'Bengal' and 'Wells' were the top-yielding conventional cultivars whereas 'Lemont' and 'CL171AR' yielded the least. Weed suppression by PI 312777 and CLXL729 under "medium" weed management was equivalent to that of Lemont and CL171AR at the "high" management level, suggesting that the weed-suppressive cultivars may be able to compensate for suboptimal herbicide inputs or incomplete weed control. C1 [Gealy, David R.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Anders, Merle; Watkins, Bradley] Univ Arkansas, Rice Res & Extens Ctr, Div Agr, Stuttgart, AR 72160 USA. [Duke, Sara] USDA ARS, Southern Plains Area, Area Statistician, College Stn, TX 77845 USA. RP Gealy, DR (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM David.Gealy@ars.usda.gov NR 62 TC 4 Z9 4 U1 5 U2 29 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2014 VL 62 IS 2 BP 303 EP 320 DI 10.1614/WS-D-13-00104.1 PG 18 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AG4BL UT WOS:000335363600010 ER PT J AU Kremer, RJ AF Kremer, Robert J. TI Environmental Implications of Herbicide Resistance: Soil Biology and Ecology SO WEED SCIENCE LA English DT Article DE deleterious rhizobacteria; microbial ecology; mycorrhizae; rhizodeposition; rhizosphere; soil microbial community; systemic herbicides; transgenic crops ID ARBUSCULAR-MYCORRHIZAL FUNGI; HORSEWEED CONYZA-CANADENSIS; CHENOPODIUM-ALBUM BIOTYPES; RAGWEED AMBROSIA-TRIFIDA; MICROBIAL COMMUNITIES; ROOT COLONIZATION; RHIZOSPHERE SOIL; WEED MANAGEMENT; LOLIUM-RIGIDUM; PLANT-GROWTH AB Soil microbial community structure and activity are linked to plant communities. Weeds may alter their soil environment, selecting for specific rhizosphere microbial communities. Rhizosphere modification occurs for many crop and horticultural plants. However, impacts of weeds in agroecosystems on soil biology and ecology have received less attention because effective weed management practices were developed to minimize their impacts on crop production. The recent development of herbicide resistance (HR) in several economically important weeds leading to widespread infestations in crop fields treated with a single herbicide has prompted a re-evaluation of the effects of weed growth on soil biology and ecology. The objective of this article is to review the potential impacts of herbicide-resistant weeds on soil biological and ecological properties based on reports for crops, weeds, and invasive plants. Persistent weed infestations likely establish extensive root systems and release various plant metabolites through root exudation. Many exudates are selective for specific soil microbial groups mediating biochemical and nutrient acquisition processes. Exudates may stimulate development of microbial groups beneficial to weed but detrimental to crop growth or beneficial to both. Changes in symbiotic and associative microbial interactions occur, especially for arbuscular mycorrhizal fungi (AMF) that are important in plant uptake of nutrients and water, and protecting from phytopathogens. Mechanisms used by weeds to disrupt symbioses in crops are not clearly described. Many herbicide-resistant weeds including Amaranthus and Chenopodium do not support AMF symbioses, potentially reducing AMF propagule density and establishment with crop plants. Herbicides applied to control HR weeds may compound effects of weeds on soil microorganisms. Systemic herbicides released through weed roots may select microbial groups that mediate detrimental processes such as nutrient immobilization or serve as opportunistic pathogens. Understanding complex interactions of weeds with soil microorganisms under extensive infestations is important in developing effective management of herbicide-resistant weeds. C1 [Kremer, Robert J.] USDA ARS, Washington, DC 20250 USA. [Kremer, Robert J.] Univ Missouri, Dept Soil Environm & Atmospher Sci, Columbia, MO 65211 USA. RP Kremer, RJ (reprint author), USDA ARS, Washington, DC 20250 USA. EM bob.kremer@ars.usda.gov FU USDA-ARS; USDAARS or the University of Missouri FX Financial and technical support provided by the USDA-ARS for completing much of the background research on which this review is based is acknowledged. Trade names are used for the benefit of the reader and do not imply endorsement of products by the USDAARS or the University of Missouri. NR 80 TC 5 Z9 6 U1 8 U2 100 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2014 VL 62 IS 2 BP 415 EP 426 DI 10.1614/WS-D-13-00114.1 PG 12 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AG4BL UT WOS:000335363600021 ER PT J AU Shaner, DL AF Shaner, Dale L. TI Lessons Learned From the History of Herbicide Resistance SO WEED SCIENCE LA English DT Article DE Herbicide-resistance; weed management ID COMMON GROUNDSEL; EVOLUTION; MANAGEMENT; ATRAZINE; FITNESS; WEEDS AB The selection of herbicide-resistant weed populations began with the introduction of synthetic herbicides in the late 1940s. For the first 20 years after introduction, there were limited reported cases of herbicide-resistant weeds. This changed in 1968 with the discovery of triazine-resistant common groundsel. Over the next 15 yr, the cases of herbicide-resistant weeds increased, primarily to triazine herbicides. Although triazine resistance was widespread, the resistant biotypes were highly unfit and were easily controlled with specific alternative herbicides. Weed scientists presumed that this would be the case for future herbicide-resistant cases and thus there was not much concern, although the companies affected by triazine resistance were somewhat active in trying to detect and manage resistance. It was not until the late 1980s with the discovery of resistance to Acetyl Co-A carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors that herbicide resistance attracted much more attention, particularly from industry. The rapid evolution of resistance to these classes of herbicides affected many companies, who responded by first establishing working groups to address resistance to specific classes of herbicides, and then by formation of the Herbicide Resistance Action Committee (HRAC). The goal of these groups, in cooperation with academia and governmental agencies, was to act as a forum for the exchange of information on herbicide-resistance selection and to develop guidelines for managing resistance. Despite these efforts, herbicide resistance continued to increase. The introduction of glyphosate-resistant crops in the 1995 provided a brief respite from herbicide resistance, and farmers rapidly adopted this relatively simple and reliable weed management system based on glyphosate. There were many warnings from academia and some companies that the glyphosate-resistant crop system was not sustainable, but this advice was not heeded. The selection of glyphosate resistant weeds dramatically changed weed management and renewed emphasis on herbicide resistance management. To date, the lesson learned from our experience with herbicide resistance is that no herbicide is invulnerable to selecting for resistant biotypes, and that over-reliance on a weed management system based solely on herbicides is not sustainable. Hopefully we have learned that a diverse weed management program that combines multiple methods is the only system that will work for the long term. C1 [Shaner, Dale L.] USDA ARS, Northern Plains Area, Ft Collins, CO 80526 USA. EM dlshaner37@gmail.com NR 22 TC 12 Z9 14 U1 2 U2 68 PU WEED SCI SOC AMER PI LAWRENCE PA 810 EAST 10TH ST, LAWRENCE, KS 66044-8897 USA SN 0043-1745 EI 1550-2759 J9 WEED SCI JI Weed Sci. PD APR-JUN PY 2014 VL 62 IS 2 BP 427 EP 431 DI 10.1614/WS-D-13-00109.1 PG 5 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AG4BL UT WOS:000335363600022 ER PT J AU Beatty, TKM Lin, BH Smith, TA AF Beatty, Timothy K. M. Lin, Biing-Hwan Smith, Travis A. TI Is Diet Quality Improving? Distributional Changes in the United States, 1989-2008 SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article ID HEALTHY EATING INDEX-2005; ONE 24-HOUR RECALL; STOCHASTIC-DOMINANCE; RISK; NUTRITION; FOOD; INFERENCE; POVERTY; CANCER; INEQUALITY AB This article measures changes in the distribution of dietary quality among adults in the United States over the period 1989-2008. Diet quality is a direct input to health, is often used as a proxy for well-being, and is an outcome variable for a wide variety of economic interventions. For the population as a whole, we find significant improvements across all levels of diet quality. Further, we find improvements for both low-income and higher-income individuals alike. Counterfactual distributions of dietary quality are constructed to investigate the extent to which observed improvements can be attributed to changes in the nutritional content of foods and to changes in population characteristics. We find that 63% of the improvement for all adults can be attributed to changes in food formulation and demographics. Changes in food formulation account for a substantially larger percentage of the dietary improvement within the lower-income population (19.6%) vs. the higher-income population (6.4%). C1 [Beatty, Timothy K. M.; Smith, Travis A.] Univ Minnesota, Dept Appl Econ, Minneapolis, MN 55455 USA. [Lin, Biing-Hwan] Econ Res Serv, USDA, Washington, DC USA. RP Beatty, TKM (reprint author), Univ Minnesota, Dept Appl Econ, Minneapolis, MN 55455 USA. FU USDA-ERS [58-4000-1-0025] FX Timothy K. M. Beatty is an associate professor in the Department of Applied Economics at the University of Minnesota. Biing-Hwan Lin is a senior economist in the Economic Research Service at the U.S. Department of Agriculture. Travis A. Smith is a PhD candidate in the Department of Applied Economics at the University of Minnesota. The authors thank two anonymous reviewers and the editor, Brian Roe, for comments and suggestions that greatly strengthened the paper. Research for this study was supported by USDA-ERS Cooperative Agreement No. 58-4000-1-0025. The views expressed here are those of the authors and cannot be attributed to the Economic Research Service or the U.S. Department of Agriculture. All remaining errors are our own. NR 54 TC 4 Z9 4 U1 2 U2 12 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD APR PY 2014 VL 96 IS 3 BP 769 EP 789 DI 10.1093/ajae/aat104 PG 21 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA AF2XF UT WOS:000334575100009 ER PT J AU D'Souza, A Jolliffe, D AF D'Souza, Anna Jolliffe, Dean TI Food Insecurity in Vulnerable Populations: Coping with Food Price Shocks in Afghanistan SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE Afghanistan; food price shock; food security; nutrition; poverty; wheat; unconditional quantile regression ID CHILD NUTRITIONAL-STATUS; DIETARY DIVERSITY; REGRESSION QUANTILES; CFA FRANC; CONSUMPTION; POVERTY; MALNUTRITION; DEVALUATION; VARIETY; INCOME AB Based on data from Afghanistan collected prior to and during the 2007-2008 food price crisis, this paper illustrates that caloric intake is an ineffectual indicator for monitoring the onset of food insecurity. Unconditional Quantile Regression estimates indicate that the most vulnerable of households, which cannot afford to make substantial cuts to calories, exhibit no decline in caloric intake in response to increasing wheat prices. In contrast, households with high-calorie diets experience large declines. The estimates also reveal declines in dietary diversity across the entire distribution of households. The most vulnerable households may be sacrificing diet quality to maintain calories, with the potential for serious and long-term health consequences. C1 [D'Souza, Anna] Econ Res Serv, USDA, Washington, DC 20024 USA. [Jolliffe, Dean] World Bank, Washington, DC USA. [Jolliffe, Dean] Inst Study Labor, Bonn, Germany. [Jolliffe, Dean] Univ Michigan, Ford Sch Publ Policy, Natl Poverty Ctr, Ann Arbor, MI 48109 USA. RP D'Souza, A (reprint author), Econ Res Serv, USDA, Washington, DC 20024 USA. EM anna.dsouza@gmail.com FU Australian Aid and the World Bank's Research Support Budget FX Anna D'Souza is a research economist at the Economic Research Service, U.S. Department of Agriculture. Dean Jolliffe is a senior economist at the World Bank, and also holds affiliations with the Institute for the Study of Labor in Bonn, Germany, and the National Poverty Center at the Ford School of Public Policy, University of Michigan. The authors are grateful to the Central Statistics Organization, Government of Afghanistan (GoA), for granting access to the data for poverty research. Findings from this analysis provide background information for the World Bank's poverty assessment for Afghanistan, and have informed the GoA Ministry of Economy. The authors wish to thank for comments the editor, Ed Taylor, and two anonymous reviewers, Maurice Landes, Ismail Rahimi, Hans G.P. Jansen, seminar participants at the World Bank-Kabul office, and conference participants at the AAEA 2011 annual meeting. Jolliffe gratefully acknowledges support from Australian Aid and the World Bank's Research Support Budget. The views expressed here are those of the authors and may not be attributed to the Economic Research Service, the U.S. Department of Agriculture, the World Bank, the Institute for the Study of Labor, or the National Poverty Center. Correspondence may be sent to: anna.dsouza@gmail.com. NR 73 TC 4 Z9 4 U1 4 U2 25 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD APR PY 2014 VL 96 IS 3 BP 790 EP 812 DI 10.1093/ajae/aat089 PG 23 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA AF2XF UT WOS:000334575100010 ER PT J AU Key, N McBride, WD AF Key, Nigel McBride, William D. TI Sub-therapeutic Antibiotics and the Efficiency of US Hog Farms SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE antibiotics; technical efficiency; productivity; hog production; antimicrobial resistance; stochastic production frontier ID PROPENSITY SCORE; REGRESSION ADJUSTMENT; DAMAGE-CONTROL; PRODUCTIVITY; GROWTH; ECONOMETRICS; AGRICULTURE; CAUSAL; TESTS; MODEL AB A substantial share of U.S. hog producers incorporate antimicrobial drugs into their livestock's feed or water at sub-therapeutic levels to promote feed efficiency and weight gain. Recently, in response to concerns that the overuse of antibiotics in livestock could promote the development of antimicrobial drug-resistant bacteria, the U.S. Food and Drug Administration adopted a strategy to phase out the use of antibiotics for production purposes. This study uses a stochastic frontier model and data from the 2009 USDA Agricultural Resource Management Survey of feeder-to-finish hog producers to estimate the potential effects on hog output and output variability resulting from a ban on antibiotics used for growth promotion. We use propensity score nearest neighbor matching to create a balanced sample of sub-therapeutic antibiotic (STA) users and nonusers. We estimate the frontier model for the pooled sample and separately for users and non-users-which allows for a flexible interaction between STA use and the production technology. Point estimates for the matched sample indicate that STA use has a small positive effect on productivity and production risk, increasing output by 1.0-1.3% and reducing the standard deviation of unexplained output by 1.4%. The results indicate that improvements in productivity resulted exclusively from technological improvement rather than from an increase in technical efficiency. C1 [Key, Nigel; McBride, William D.] Econ Res Serv, USDA, Washington, DC 20024 USA. RP Key, N (reprint author), Econ Res Serv, USDA, Washington, DC 20024 USA. EM nkey@ers.usda.gov NR 38 TC 7 Z9 8 U1 3 U2 36 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD APR PY 2014 VL 96 IS 3 BP 831 EP 850 DI 10.1093/ajae/aat091 PG 20 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA AF2XF UT WOS:000334575100012 ER PT J AU Fuglie, KO Toole, AA AF Fuglie, Keith O. Toole, Andrew A. TI The Evolving Institutional Structure of Public and Private Agricultural Research SO AMERICAN JOURNAL OF AGRICULTURAL ECONOMICS LA English DT Article DE Agricultural Biotechnology; Agricultural Input Industries; Complementarity; CRADA; Institutional Change; Pasteur's Quadrant; Patent Licensing; Public-private Technology Transfer; Research Consortia ID RESEARCH-AND-DEVELOPMENT; INNOVATION RESEARCH-PROGRAM; DEVELOPMENT CONSORTIA; UNITED-STATES; SBIR PROGRAM; INVESTMENT; INDUSTRY; GOVERNMENT; COLLABORATION; INCENTIVES AB Over the past several decades, the private sector has assumed a larger role in developing improved technology for food and agriculture. Private companies fund nearly all food processing research and development (R&D) and perform a growing share of production-oriented R&D for agriculture. In addition, institutional partnerships for public-private research collaboration are growing in the United States and other countries. This article outlines the major forces driving these changes and offers an interpretive framework to explore some of the implications for the volume and nature of research performed by the public and private sectors. One of the critical issues is whether public agricultural research complements and thereby stimulates additional private agricultural R&D investments. Another important issue concerns the role and contribution of alternative public-private partnership arrangements. To date, changes in the institutional structure of public and private agricultural research have outpaced systematic investigation, and new theoretical and empirical research is needed to help guide policy and address key societal challenges, such as climate change, clean energy, water scarcity, food safety, and health. C1 [Fuglie, Keith O.; Toole, Andrew A.] Econ Res Serv, USDA, Washington, DC 20024 USA. [Toole, Andrew A.] Ctr European Econ Res ZEW, Mannheim, Germany. RP Fuglie, KO (reprint author), Econ Res Serv, USDA, Washington, DC 20024 USA. EM kfuglie@ers.usda.gov NR 61 TC 8 Z9 9 U1 4 U2 41 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0002-9092 EI 1467-8276 J9 AM J AGR ECON JI Am. J. Agr. Econ. PD APR PY 2014 VL 96 IS 3 BP 862 EP 883 DI 10.1093/ajae/aat107 PG 22 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA AF2XF UT WOS:000334575100014 ER PT J AU Bergdale, TE Hughes, SR Bang, SS AF Bergdale, Terran E. Hughes, Stephen R. Bang, Sookie S. TI Thermostable Hemicellulases of a Bacterium, Geobacillus sp DC3, Isolated from the Former Homestake Gold Mine in Lead, South Dakota SO APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY LA English DT Article DE Geobacillus; Extremophile; Xylanase; Betaxylosidase; Arabinofuranosidase ID BACILLUS-THERMOGLUCOSIDASIUS; THERMOPHILIC BACILLI; CATALYTIC-PROPERTIES; MICROBIAL XYLANASES; DEGRADING ENZYMES; SP-NOV; PURIFICATION; STRAIN; STEAROTHERMOPHILUS; THERMOLEOVORANS AB A thermophilic strain, Geobacillus sp. DC3, capable of producing hemicellulolytic enzymes was isolated from the 1.5-km depth of the former Homestake gold mine in Lead, South Dakota. The DC3 strain expressed a high level of extracellular endoxylanase at 39.5 U/mg protein with additional hemicellulases including beta-xylosidase (0.209 U/mg) and arabinofuranosidase (0.230 U/mg), after the bacterium was grown in xylan for 24 h. Partially purified DC3 endoxylanase exhibited a molecular mass of approximately 43 kDa according to zymography with an optimal pH of 7 and optimal temperature of 70 A degrees C. The kinetic constants, K (m) and V (max), were 13.8 mg/mL and 77.5 mu mol xylose/min center dot mg xylan, respectively. The endoxylanase was highly stable and maintained 70 % of its original activity after 16 h incubation at 70 A degrees C. The thermostable properties and presence of three different hemicellulases of Geobacillus sp. DC3 strain support its potential application for industrial hydrolysis of renewable biomass such as lignocelluloses. C1 [Bergdale, Terran E.; Bang, Sookie S.] South Dakota Sch Mines & Technol, Dept Chem & Biol Engn, Rapid City, SD 57701 USA. [Hughes, Stephen R.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Bang, SS (reprint author), South Dakota Sch Mines & Technol, Dept Chem & Biol Engn, 501 East St Joseph St, Rapid City, SD 57701 USA. EM sookie.bang@sdsmt.edu FU South Dakota NASA EPSCoR Research Infrastructure Development (RID) Grant [NNX07AL04A]; South Dakota Board of Regents [SDBOR/BHSU 2011-19005]; Black Hills State University [BHSU/SDSMT BA1100002]; South Dakota Center of Bioprocessing Research and Development FX The authors wish to thank Dr. Cynthia Anderson and Dr. Dave Bergmann at Black Hills State University for providing valuable help in obtaining and interpreting sequencing data. Additional thanks go toward Dr. Todd Menkhaus for lending equipment for this project. Part of this work was supported by the South Dakota NASA EPSCoR Research Infrastructure Development (RID) Grant No. NNX07AL04A and South Dakota Board of Regents (SDBOR/BHSU 2011-19005) as a subaward from Black Hills State University (BHSU/SDSMT BA1100002). Additional support was received from the South Dakota 2010 Center of Bioprocessing Research and Development. NR 61 TC 1 Z9 1 U1 2 U2 15 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DRIVE SUITE 208, TOTOWA, NJ 07512 USA SN 0273-2289 EI 1559-0291 J9 APPL BIOCHEM BIOTECH JI Appl. Biochem. Biotechnol. PD APR PY 2014 VL 172 IS 7 BP 3488 EP 3501 DI 10.1007/s12010-014-0784-7 PG 14 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA AF0VQ UT WOS:000334432800015 PM 24549802 ER PT J AU Sands, R Forster, H Jones, C Schumacher, K AF Sands, Ronald D. Foerster, Hannah Jones, Carol A. Schumacher, Katja TI Bio-electricity and land use in the Future Agricultural Resources Model (FARM) SO CLIMATIC CHANGE LA English DT Article ID ENERGY AB Bio-electricity is an important technology for Energy Modeling Forum (EMF-27) mitigation scenarios, especially with the possibility of negative carbon dioxide emissions when combined with carbon dioxide capture and storage (CCS). With a strong economic foundation, and broad coverage of economic activity, computable general equilibrium models have proven useful for analysis of alternative climate change policies. However, embedding energy technologies in a general equilibrium model is a challenge, especially for a negative emissions technology with joint products of electricity and carbon dioxide storage. We provide a careful implementation of bio-electricity with CCS in a general equilibrium context, and apply it to selected EMF-27 mitigation scenarios through 2100. Representing bio-electricity and its land requirements requires consideration of competing land uses, including crops, pasture, and forests. Land requirements for bio-electricity start at 200 kilohectares per terawatt-hour declining to approximately 70 kilohectares per terwatt-hour by year 2100 in scenarios with high bioenergy potential. C1 [Sands, Ronald D.; Jones, Carol A.] USDA, Econ Res Serv, Washington, DC 20250 USA. [Foerster, Hannah; Schumacher, Katja] Oko Inst, D-10179 Berlin, Germany. RP Sands, R (reprint author), USDA, Econ Res Serv, 1400 Independence Ave,SW,Mail Stop 1800, Washington, DC 20250 USA. EM rsands@ers.usda.gov NR 14 TC 4 Z9 4 U1 1 U2 10 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD APR PY 2014 VL 123 IS 3-4 SI SI BP 719 EP 730 DI 10.1007/s10584-013-0943-9 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AF0OW UT WOS:000334414900027 ER PT J AU Cervantes, JHF Istanbulluoglu, E Vivoni, ER Collins, CDH Bras, RL AF Cervantes, Javier H. Flores Istanbulluoglu, Erkan Vivoni, Enrique R. Collins, Chandra D. Holifield Bras, Rafael L. TI A geomorphic perspective on terrain- modulated organization of vegetation productivity: analysis in two semiarid grassland ecosystems in Southwestern United States SO ECOHYDROLOGY LA English DT Article DE geomorphology; productivity; grassland; topography ID LANDSCAPE EVOLUTION; DRAINAGE DENSITY; MODEL; TOPOGRAPHY; MORPHOLOGY; ELEVATION; DYNAMICS; GRADIENT; IMAGERY; RUNOFF AB Spatial patterns of ecosystem productivity arise from the terrain-modulated wetting and drying of the landscape. Using a daily relative greenness (rG) index, we explore the relations between spatial variability of plant productivity and landscape morphology, and how these relations change over time. The rG index is defined as a measure of local vegetation greenness relative to the site's mean greenness, calculated from remotely sensed normalized difference vegetation index. We analyse two semiarid grasslands with pronounced topography, one located in southeastern Arizona, with a mean annual precipitation of 350 mm, and the other in central New Mexico, with a mean annual precipitation of 250 mm. Our results indicate that (1) rG is spatially more uniform after wet conditions (higher biomass) than after dry conditions (lower biomass); (2) differences in the relative frequency distribution of rG among different landscape morphologies (ridges, unchanneled valleys and channels) indicate higher productivity in channels, similar coefficient of variation in all process domains, and higher skewness in the ridges; (3) relatively high correlations between the binned average rG with respect to upstream area, curvature, and annual insolation in more than 80% of the terrain indicate a clear dependence between ecosystem productivity and topography; (4) rG is more sensitive to changes in topographic indices at the wetter Arizona study site. Such improved understanding of vegetation-topography dependence is critical for ecosystem management, testing ecohydrologic models, and offers ideas for the downscaling of coarse-scale satellite-derived vegetation indices. Copyright (c) 2012 John Wiley & Sons, Ltd. C1 [Cervantes, Javier H. Flores; Istanbulluoglu, Erkan] Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA. [Vivoni, Enrique R.] Arizona State Univ, Sch Earth & Space Explorat, Tempe, AZ 85287 USA. [Vivoni, Enrique R.] Arizona State Univ, Sch Sustainable Engn & Built Environm, Tempe, AZ 85287 USA. [Collins, Chandra D. Holifield] ARS, Southwest Watershed Res Ctr, USDA, Tucson, AZ 85719 USA. [Bras, Rafael L.] Georgia Inst Technol, Sch Civil & Environm Engn, Atlanta, GA 30332 USA. [Bras, Rafael L.] Georgia Inst Technol, Sch Earth & Planetary Sci, Atlanta, GA 30332 USA. RP Cervantes, JHF (reprint author), Univ Washington, Dept Civil & Environm Engn, Seattle, WA 98195 USA. EM homefc@uw.edu RI Vivoni, Enrique/E-1202-2012 OI Vivoni, Enrique/0000-0002-2659-9459 FU NSF [EAR-0963858, EAR 0642550] FX We acknowledge the support of NSF through the grants EAR-0963858 (Istanbulluoglu) and NSF EAR 0642550 (Bras). We are thankful for the Kendall data provided by the Southwest Watershed Research Centre (SWRC) and by the Sevilleta Long Term Ecological Research Programme. The authors would also like to thank two anonymous reviewers for their valuable comments and suggestions that eventually lead to an overall improvement of this manuscript. NR 49 TC 5 Z9 5 U1 3 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD APR PY 2014 VL 7 IS 2 BP 242 EP 257 DI 10.1002/eco.1333 PG 16 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA AF4HC UT WOS:000334671600006 ER PT J AU Niu, GY Paniconi, C Troch, PA Scott, RL Durcik, M Zeng, XB Huxman, T Goodrich, DC AF Niu, Guo-Yue Paniconi, Claudio Troch, Peter A. Scott, Russell L. Durcik, Matej Zeng, Xubin Huxman, Travis Goodrich, David C. TI An integrated modelling framework of catchment- scale ecohydrological processes: 1. Model description and tests over an energy- limited watershed SO ECOHYDROLOGY LA English DT Article DE coupling; physically-based hydrological model; land surface model; ecohydrological model; snowmelt runoff ID DIGITAL ELEVATION MODELS; GROUNDWATER-FLOW MODEL; LAND-SURFACE SCHEME; HYDROLOGIC MODEL; STOMATAL CONDUCTANCE; CONTINENTAL-SCALE; CLIMATE MODEL; PART I; PARAMETERIZATION; REPRESENTATION AB The interactions between atmospheric, hydrological, and ecological processes at various spatial and temporal scales are not fully represented in most ecohydrological models. This first of a two-part paper documents a fully integrated catchment-scale ecohydrological model consisting of a three-dimensional physically based hydrological model and a land surface model. This first part also presents a first application to test the model over an energy-limited catchment (8.4km(2)) of the Sleepers River watershed in Vermont. The physically based hydrological model (CATchment HYdrology, CATHY) describes three-dimensional subsurface flow in variably saturated porous media and surface routing on hillslopes and in stream channels, whereas the land surface model (LSM), an augmented version of Noah LSM with multiple parameterization schemes (NoahMP), accounts for energy, water, and carbon flux exchanges between various land surface elements and the atmosphere. CATHY and NoahMP are coupled through exchanges of water fluxes and states. In the energy-limited catchment of the Sleepers River watershed, where snowmelt runoff generation is the dominant hydrologic flux, the coupled CATHY/NoahMP model at both 90 and 30-m surface grid resolutions, with minimal calibration, performs well in simulating the observed snow accumulation, and melt and subsequent snowmelt discharge. The Nash-Sutcliffe model efficiency of daily discharge is above 0.82 for both resolutions. The simulation at 90-m resolution shows a marginal improvement over that at 30-m resolution because of more elaborate calibration of model parameters. The coupled CATHY/NoahMP also shows a capability of simulating surface-inundated area and distributed surface water height, although the accuracy of these simulations needs further evaluation. The CATHY/NoahMP model is thus also a potentially useful research tool for predicting flash flood and lake dynamics under climatic change. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Niu, Guo-Yue; Troch, Peter A.; Durcik, Matej; Zeng, Xubin; Huxman, Travis] Univ Arizona, Tucson, AZ 85721 USA. [Paniconi, Claudio] Univ Quebec, Inst Natl Rech Sci, Ctr Eau Terre & Environm, Quebec City, PQ, Canada. [Troch, Peter A.] Univ Arizona, Dept Hydrol & Water Resources, Tucson, AZ 85721 USA. [Scott, Russell L.; Goodrich, David C.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Durcik, Matej] Univ Arizona, SAHRA Sustainabil Semiarid Hydrol & Riparian Area, Tucson, AZ 85721 USA. [Zeng, Xubin] Univ Arizona, Dept Atmospher Sci, Tucson, AZ 85721 USA. [Huxman, Travis] Univ Arizona, Dept Ecol & Evolutionary Biol, Tucson, AZ 85721 USA. RP Niu, GY (reprint author), POB 8746, Tucson, AZ 85738 USA. EM niug@email.arizona.edu RI Durcik, Matej/F-2266-2010; Niu, Guo-Yue/B-8317-2011; OI Durcik, Matej/0000-0002-7064-961X; Zeng, Xubin/0000-0001-7352-2764; Paniconi, Claudio/0000-0003-2063-2841 FU Biosphere 2; DOE [DE-SC0006773]; NSF [EF 1065790] FX This work was mainly supported by Biosphere 2 and partly by DOE (DE-SC0006773) and NSF (EF 1065790). We thank Marc Stieglitz for providing us with the Sleepers River watershed dataset. NR 73 TC 23 Z9 23 U1 2 U2 46 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD APR PY 2014 VL 7 IS 2 BP 427 EP 439 DI 10.1002/eco.1362 PG 13 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA AF4HC UT WOS:000334671600021 ER PT J AU Williams, CJ Pierson, FB Al-Hamdan, OZ Kormos, PR Hardegree, SP Clark, PE AF Williams, C. Jason Pierson, Frederick B. Al-Hamdan, Osama Z. Kormos, Patrick R. Hardegree, Stuart P. Clark, Patrick E. TI Can wildfire serve as an ecohydrologic threshold- reversal mechanism on juniper- encroached shrublands SO ECOHYDROLOGY LA English DT Review DE ecohydrologic resilience; infiltration; runoff; sagebrush steppe; soil erosion feedback; thresholds; western juniper; woodland encroachment ID SOIL-WATER REPELLENCY; CROSS-SCALE INTERACTIONS; WESTERN UNITED-STATES; NEW-MEXICO; TRANSITION MODELS; SAGEBRUSH STEPPE; RANGELAND HEALTH; SOUTHERN ARIZONA; BROMUS-TECTORUM; PRESCRIBED-FIRE AB Woody plant encroachment on water-limited lands can induce a shift from biotic (plant)-controlled resource retention to abiotic (physical)-driven losses of critical soil resources. The biotic-to-abiotic shift occurs where encroachment propagates connectivity of runoff processes and amplified cross-scale erosion that, in-turn, promote ecohydrologic resilience of the post-encroachment community. We investigated these relationships for woodland-encroached sagebrush steppe in the Great Basin, USA, and evaluated wildfire as a mechanism to reverse the post-encroachment soil erosion feedback. We measured vegetation, soil properties, and runoff/erosion from experimental plots on burned and unburned areas of a late-succession woodland 1 and 2years post-fire. Our findings suggest that the biotic-to-abiotic shift and amplified cross-scale erosion occur where encroachment-induced bare ground exceeds 50-60% and bare gaps between plant bases frequently extend beyond 1m. The trigger for amplified cross-scale erosion is formation of concentrated flow within the degraded intercanopy between trees. Burning in this study decreased ecohydrologic resilience of the late-succession woodland through herbaceous recruitment 2years post-fire. Increased intercanopy herbaceous productivity decreased connectivity of bare ground, improved infiltration, and reduced erosion, but the study site remained vulnerable to runoff and erosion from high-intensity rainfall. We conclude that burning can reduce woodland ecohydrologic resilience and that woodland encroachment-induced structural and functional ecohydrologic attributes may persist during high-intensity storms for an undetermined period post-fire. We cannot conclude whether wildfire reverses the woodland-induced soil erosion feedback on sagebrush rangelands. However, our results suggest that wildfire may provide a restoration pathway for sagebrush steppe by reducing woodland ecohydrologic resilience over time. Published 2013. This article is a U.S. Government work and is in the public domain in the USA. C1 [Williams, C. Jason; Pierson, Frederick B.; Al-Hamdan, Osama Z.; Hardegree, Stuart P.; Clark, Patrick E.] ARS, USDA, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Al-Hamdan, Osama Z.] Univ Idaho, Dept Biol & Agr Engn, Moscow, ID 83844 USA. [Kormos, Patrick R.] Boise State Univ, Dept Geosci, Boise, ID 83725 USA. RP Williams, CJ (reprint author), ARS, USDA, Northwest Watershed Res Ctr, 800 Pk Blvd,Suite 105, Boise, ID 83712 USA. EM jason.williams@ars.usda.gov OI Kormos, Patrick/0000-0003-1874-9215; Clark, Patrick/0000-0003-4299-1853; Williams, Jason/0000-0002-6289-4789 FU US Joint Fire Science Program FX This is Contribution Number 76 of the Sagebrush Treatment Evaluation Project (SageSTEP), funded by the US Joint Fire Science Program. The authors wish to thank Jaime Calderon and Mathew Frisby for assistance with data collection. We thank Ben Rau (Soil Scientist, USDA Agricultural Research Service) and the Desert Research Institute, Reno, Nevada, for assistance with processing soil samples. We thank Bruce Mackey (Statistician, USDA Agricultural Research Service) for assistance with components of the statistical analyses. NR 149 TC 19 Z9 20 U1 3 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD APR PY 2014 VL 7 IS 2 BP 453 EP 477 DI 10.1002/eco.1364 PG 25 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA AF4HC UT WOS:000334671600023 ER PT J AU Niu, GY Troch, PA Paniconi, C Scott, RL Durcik, M Zeng, XB Huxman, T Goodrich, D Pelletier, J AF Niu, Guo-Yue Troch, Peter A. Paniconi, Claudio Scott, Russell L. Durcik, Matej Zeng, Xubin Huxman, Travis Goodrich, David Pelletier, Jon TI An integrated modelling framework of catchment- scale ecohydrological processes: 2. The role of water subsidy by overland flow on vegetation dynamics in a semi- arid catchment SO ECOHYDROLOGY LA English DT Article DE overland flow; re-infiltration; vegetation dynamics; semi-arid catchment ID GENERAL-CIRCULATION MODELS; NORTH-AMERICA; CLIMATE; VARIABILITY; SAHEL; DESERTIFICATION AB In water-limited regions, surface water and carbon fluxes are strongly controlled by soil water availability, which may be highly variable at very small spatial scales (e.g. metres) because of variations in terrain, soils, and vegetation conditions and to processes of water redistribution along hillslopes. This second of a two-part paper first evaluates the performance of a newly developed ecohydrological model over a small semi-arid experimental catchment (792ha) in southeastern Arizona. Secondly, it investigates the effects of soil properties on water subsidy resulting from lateral overland flow re-infiltration and on overall ecohydrological response. With optimized parameters, the model shows a higher ability to simulate surface energy and water fluxes than CO2 fluxes at all temporal scales. The model simulates observed CO2 fluxes fairly well at diurnal scales during the main growing seasons and the interannual variability of these fluxes in response to soil moisture variations from drought years to wet years. However, the model reproduces less well carbon assimilation in spring and positive CO2 flux pulses following early monsoon rain events, suggesting a need for further development of the model's representations of multiple plant species and soil carbon decomposition. The model simulates soil moisture at 5cm much better than at 15cm mainly because of heterogeneous soil properties. Through five numerical experiments with varying saturated hydraulic conductivity values, it is revealed that the discharge at the outlet of this semi-arid catchment is essentially attributed to lateral overland flow that is generated mainly by infiltration-excess runoff. Subsurface flow plays a minor role in this semi-arid catchment with a very deep groundwater table (>100m). The model produces wetter soils in lowland areas along stream rills and channels through re-infiltration of lateral overland flow. This water subsidy provides plants with favourable conditions to produce more leaves, CO2, and ET fluxes in lowland areas. Re-infiltration of overland flow over complex terrain may play a role in buffering climatic impacts in a warming climate with fewer but more intense rainfall events in the Southwestern United States. Copyright (c) 2013 John Wiley & Sons, Ltd. C1 [Niu, Guo-Yue; Troch, Peter A.; Durcik, Matej; Zeng, Xubin; Pelletier, Jon] Univ Arizona, Tucson, AZ 85738 USA. [Troch, Peter A.] Univ Arizona, Dept Hydrol & Water Resources, Tucson, AZ 85738 USA. [Paniconi, Claudio] Univ Quebec, Ctr Eau Terre Environm, Inst Natl Rech Sci INRS ETE, Quebec City, PQ, Canada. [Scott, Russell L.; Goodrich, David] ARS, Southwest Watershed Res Ctr, USDA, Tucson, AZ USA. [Durcik, Matej] Univ Arizona, SAHRA Sustainabil Semiarid Hydrol & Riparian Area, Tucson, AZ 85738 USA. [Zeng, Xubin] Univ Arizona, Dept Atmospher Sci, Tucson, AZ 85738 USA. [Huxman, Travis] Univ Calif Irvine, Sch Biol Sci, Irvine, CA 92717 USA. [Pelletier, Jon] Univ Arizona, Dept Geosci, Tucson, AZ 85738 USA. RP Niu, GY (reprint author), Univ Arizona, POB 8746, Tucson, AZ 85738 USA. EM niug@email.arizona.edu RI Durcik, Matej/F-2266-2010; Niu, Guo-Yue/B-8317-2011; OI Durcik, Matej/0000-0002-7064-961X; Zeng, Xubin/0000-0001-7352-2764; Paniconi, Claudio/0000-0003-2063-2841 FU Biosphere 2; DOE [DE-SC0006773]; NSF [EF 1065790]; EPA; US Department of Defense Legacy Program FX This work was mainly supported by Biosphere 2 and partly by DOE (DE-SC0006773) and NSF (EF 1065790). Hydrologic, watershed characterization, and flux data were provided by the USDA-ARS, Southwest Watershed Research Center (SWRC), Tucson, AZ. LIDAR data were provided by the SWRC and the University of Florida with funding assistance from the EPA and the US Department of Defense Legacy Program. We commend and gratefully acknowledge the dedication of the ARS Southwest Watershed Research Center for maintaining the Walnut Gulch Experimental Watershed and for their diligent long-term collection of high-quality hydrologic and watershed data. We also wish to thank the two anonymous reviewers for their very helpful comments on the paper. NR 42 TC 5 Z9 5 U1 0 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD APR PY 2014 VL 7 IS 2 BP 815 EP 827 DI 10.1002/eco.1405 PG 13 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA AF4HC UT WOS:000334671600050 ER PT J AU O'Neil, GW Knothe, G Williams, JR Burlow, NP Culler, AR Corliss, JM Carmichael, CA Reddy, CM AF O'Neil, Gregory W. Knothe, Gerhard Williams, John R. Burlow, Noah P. Culler, Aaron R. Corliss, Joshua M. Carmichael, Catherine A. Reddy, Christopher M. TI Synthesis and Analysis of an Alkenone-Free Biodiesel from Isochrysis sp. SO ENERGY & FUELS LA English DT Article ID FATTY-ACID PROFILE; FUEL PROPERTIES; METHYL-ESTERS; ISOTOPIC COMPOSITION; D/H FRACTIONATION; EMILIANIA-HUXLEYI; MICROALGAE; OIL; LUBRICITY; SALINITY AB Some marine microalgae, such as Isochrysis sp., produce high-melting (similar to 70 degrees C) lipids known as long-chain alkenones that detrimentally affect biodiesel fuel quality. A method has been developed for the production of an alkenone-free Isochrysis biodiesel. This material was prepared on sufficient scale to allow for extensive analysis according to ASTM standards. Results revealed that while cold flow improved by removal of these high-melting components and lubricity and glycerol content were below maximum levels prescribed in the standards, other properties dependent on fatty acid methyl ester (FAME) structure and composition such as oxidative stability failed to meet ASTM specifications. Both the cetane number and kinematic viscosity were lower than what would be calculated based on FAME content. This has been explained in part by 6-8% non-FAME contaminants in the tested samples. C1 [O'Neil, Gregory W.; Williams, John R.; Burlow, Noah P.; Culler, Aaron R.; Corliss, Joshua M.] Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA. [Knothe, Gerhard] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Carmichael, Catherine A.; Reddy, Christopher M.] Woods Hole Oceanog Inst, Dept Marine Chem & Geochem, Woods Hole, MA 02543 USA. RP O'Neil, GW (reprint author), Western Washington Univ, Dept Chem, Bellingham, WA 98225 USA. EM oneil@chem.wwu.edu OI Burlow, Noah/0000-0001-9659-2810 FU National Science Foundation [CHE-1151492] FX This work was supported by the National Science Foundation (CHE-1151492). We thank Kevin R. Steidley and Kim Ascherl (USDA/ARS/NCAUR) for excellent technical assistance, Dr. Karl Vermillion (USDA/ARS/NCAUR) for obtaining the NMR spectra, and Barrett Mangold (Southwest Research Institute, San Antonio, TX) for cetane testing. NR 53 TC 5 Z9 5 U1 1 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD APR PY 2014 VL 28 IS 4 BP 2677 EP 2683 DI 10.1021/ef500246h PG 7 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA AF5CD UT WOS:000334730700042 ER PT J AU Dawson-Hughes, B Harris, SS Ceglia, L Palermo, NJ AF Dawson-Hughes, Bess Harris, Susan S. Ceglia, Lisa Palermo, Nancy J. TI Effect of supplemental vitamin D and calcium on serum sclerostin levels SO EUROPEAN JOURNAL OF ENDOCRINOLOGY LA English DT Article ID BONE-MINERAL DENSITY; CIRCULATING SCLEROSTIN; PARATHYROID-HORMONE; WOMEN; OLDER; MEN; TURNOVER; FRACTURE; RISK; MASS AB Objective: Serum sclerostin levels have been reported to be inversely associated with serum 25OHD levels, but the effect of vitamin D and calcium supplementation on serum sclerostin levels is unknown. This study was carried out to determine whether vitamin D and calcium supplementation altered serum sclerostin levels in healthy older adults. Design: We measured serum sclerostin levels at baseline and after 2 years in 279 men and women who participated in a placebo-controlled vitamin D (700 IU/day) and calcium (500 mg/day) intervention trial carried out in men and women aged >= 65 years. Method: Serum sclerostin levels were measured using the MesoScale Discovery chemiluminescence assay. Results: In the men, sclerostin levels increased over 2 years by 4.11+/-1.81 ng/l (13.1%) in the vitamin D plus calcium-supplemented group and decreased by 3.16+/-1.78 ng/l (10.9%) in the placebo group (P=0.005 for difference in change). Adjustments for the season of measurement, baseline physical activity levels, baseline serum sclerostin levels, and total body bone mineral content did not substantially alter the changes. In the women, there was no significant group difference in change in serum sclerostin levels either before or after the above-mentioned adjustments. In both the sexes, vitamin D and calcium supplementation significantly increased serum ionized calcium levels and decreased parathyroid hormone levels. Conclusion: Men and women appear to have different serum sclerostin responses to vitamin D and calcium supplementation. The reason for this difference remains to be determined. C1 [Dawson-Hughes, Bess; Harris, Susan S.; Ceglia, Lisa; Palermo, Nancy J.] Tufts Univ, Jean Mayer United States Dept Agr, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Dawson-Hughes, Bess; Ceglia, Lisa] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA 02111 USA. RP Dawson-Hughes, B (reprint author), Tufts Univ, Jean Mayer United States Dept Agr, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM Bess.Dawson-Hughes@Tufts.edu FU U.S. National Institute on Aging [AG10353]; National Institutes of Health; Amgen, Inc.; U.S. Department of Agriculture, Agricultural Research Service [58-1950-0-014] FX This study was supported by grant number AG10353 from the U.S. National Institute on Aging and the National Institutes of Health, Amgen, Inc., and the U.S. Department of Agriculture, Agricultural Research Service, under agreement no. 58-1950-0-014. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the U.S. Department of Agriculture. NR 18 TC 17 Z9 18 U1 0 U2 2 PU BIOSCIENTIFICA LTD PI BRISTOL PA EURO HOUSE, 22 APEX COURT WOODLANDS, BRADLEY STOKE, BRISTOL BS32 4JT, ENGLAND SN 0804-4643 EI 1479-683X J9 EUR J ENDOCRINOL JI Eur. J. Endocrinol. PD APR PY 2014 VL 170 IS 4 BP 645 EP 650 DI 10.1530/EJE-13-0862 PG 6 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AE9TB UT WOS:000334351800026 PM 24488080 ER PT J AU Liu, M Rodrigue, N Kolmer, J AF Liu, M. Rodrigue, N. Kolmer, J. TI Population divergence in the wheat leaf rust fungus Puccinia triticina is correlated with wheat evolution SO HEREDITY LA English DT Article DE Uredinales; phylogenetics; fungi; coalescence; host adaptation; obligate parasite ID GENETIC DIFFERENTIATION; MOLECULAR POLYMORPHISM; PHYLOGENETIC NETWORKS; VIRULENCE PHENOTYPES; STATISTICAL-METHOD; DNA POLYMORPHISM; TETRAPLOID WHEAT; STEM RUST; ISRAEL; RESISTANCE AB Co-evolution of fungal pathogens with their host species during the domestication of modern crop varieties has likely affected the current genetic divergence of pathogen populations. The objective of this study was to determine if the evolutionary history of the obligate rust pathogen on wheat, Puccinia triticina, is correlated with adaptation to hosts with different ploidy levels. Sequence data from 15 loci with different levels of polymorphism were generated. Phylogenetic analyses (parsimony, Bayesian, maximum likelihood) showed the clear initial divergence of P. triticina isolates collected from Aegilops speltoides (the likely B genome donor of modern wheat) in Israel from the other isolates that were collected from tetraploid (AB genomes) durum wheat and hexaploid (ABD genomes) common wheat. Coalescence-based genealogy samplers also indicated that P. triticina on A. speltoides, diverged initially, followed by P. triticina isolates from durum wheat in Ethiopia and then by isolates from common wheat. Isolates of P. triticina found worldwide on cultivated durum wheat were the most recently coalesced and formed a clade nested within the isolates from common wheat. By a relative time scale, the divergence of P. triticinia as delimited by host specificity appears very recent. Significant reciprocal gene flow between isolates from common wheat and isolates from durum wheat that are found worldwide was detected, in addition to gene flow from isolates on common wheat to isolates on durum wheat in Ethiopia. C1 [Liu, M.; Kolmer, J.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Rodrigue, N.] Univ Calgary, Dept Math & Stat, Calgary, AB T2N 1N4, Canada. RP Kolmer, J (reprint author), USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. EM jim.kolmer@ars.usda.gov NR 51 TC 3 Z9 3 U1 2 U2 18 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0018-067X EI 1365-2540 J9 HEREDITY JI Heredity PD APR PY 2014 VL 112 IS 4 BP 443 EP 453 DI 10.1038/hdy.2013.123 PG 11 WC Ecology; Evolutionary Biology; Genetics & Heredity SC Environmental Sciences & Ecology; Evolutionary Biology; Genetics & Heredity GA AF3IJ UT WOS:000334605000010 PM 24301080 ER PT J AU Carlson, A Dong, DS Lino, M AF Carlson, Andrea Dong, Diansheng Lino, Mark TI Association between Total Diet Cost and Diet Quality Is Limited SO JOURNAL OF AGRICULTURAL AND RESOURCE ECONOMICS LA English DT Article DE cost of food; diet quality; HEI-2005; NHANES; random-effects model ID UNITED-STATES; CHILD HEALTH; US ADULTS; INCOME; ENERGY; DEMAND; WOMEN; HEALTHFULNESS; CONSUMPTION; VEGETABLES AB There is a common perception that it costs more to eat a healthy diet than a less healthy one. We derive a panel data model that accounts for unobserved specific individual effects to estimate the relationship between diet quality and total daily food expenditure. Since total daily diet cost and diet quality are both calculated from the foods chosen in our data, we account for the fact that there is an endogenous relationship between diet quality and cost. We find that while total daily food expenditure is statistically significant in relation to diet quality, the degree of association is very small. C1 [Carlson, Andrea; Dong, Diansheng] Econ Res Serv, USDA, Food Econ Div, Food Markets Branch, Washington, DC 20250 USA. [Lino, Mark] USDA, Ctr Nutr Policy & Promot, Washington, DC USA. RP Carlson, A (reprint author), Econ Res Serv, USDA, Food Econ Div, Food Markets Branch, Washington, DC 20250 USA. NR 67 TC 2 Z9 2 U1 2 U2 7 PU WESTERN AGRICULTURAL ECONOMICS ASSOC PI LOGAN PA C/O DEEVON BAILEY, UTAH STATE UNIV, ECONOMICS DEPT, 3535 OLD MAIN HILL, LOGAN, UT 84322-3530 USA SN 1068-5502 J9 J AGR RESOUR ECON JI J. Agric. Resour. Econ. PD APR PY 2014 VL 39 IS 1 BP 47 EP 68 PG 22 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA AF7NM UT WOS:000334901800004 ER PT J AU Winger, M Khouryieh, H Aramouni, F Herald, T AF Winger, M. Khouryieh, H. Aramouni, F. Herald, T. TI SORGHUM FLOUR CHARACTERIZATION AND EVALUATION IN GLUTEN-FREE FLOUR TORTILLA SO JOURNAL OF FOOD QUALITY LA English DT Article ID ABRASIVE DEHULLING DEVICE; PASTING PROPERTIES; AMYLOSE CONTENT; QUALITY; STARCH; WHEAT; GRAIN; BREAD; TEXTURE; NOODLES AB Four sorghum hybrids (Fontanelle-625 [F-625]), Fontanelle-1000 [F-1,000], ATx631xRTx2907 [NE#20] and 5,040C) were characterized and evaluated for kernel characteristics, proximate analysis, flour characterization (particle size distributions, starch damage, amylose content and starch pasting properties) and end product in gluten-free tortilla. A commercial sorghum flour (TVM) was used as a control. Significant differences were found (P<0.05) among hybrids for kernel and flour composition except total starch (P>0.05). NE#20 had the largest particle diameter for both flour and starch. F-1000 had significantly higher starch damage compared with the other hybrids. Flours with smaller particle size and higher starch damage contributed to softer and more extensible tortilla. Amylose content ranged from 20.2 (NE#20) to 27.3 (F-1000). Tortilla made with TVM flour had the highest extensibility, while the F-625 tortilla had the lowest. F-625 tortilla had the lightest color with L* value of 70.38, while the 5,040C tortilla had the darkest with L* value of 61.68. Descriptive sensory results showed significant differences for tortilla grain specks, angle of bend, rancidity, sweetness, springiness, hardness and grittiness. The results have shown that sorghum hybrids can differ in kernel and flour properties, which could help predict sorghum flour quality for the purpose of gluten-free products. Practical ApplicationsSorghum is a gluten-free grain tolerated by patients with celiac disease, that has potential in the gluten-free food market. Despite considerable scientific progress in understanding celiac disease, to date, a strict gluten-free diet for life is the only treatment for patients with celiac disease. With an increasing number of people being diagnosed with celiac disease and with the market for gluten-free products growing, there is a great opportunity to create new products using sorghum flour. There are many sorghum hybrids that have not been characterized for grain, flour or end-product quality. Therefore, understanding the quality attributes of sorghum varieties is critical in translating to end-product use. The results have shown that sorghum hybrids can differ in kernel and flour characteristics, which could help predict end-product quality and application in gluten-free products. C1 [Winger, M.] Caravan Ingredients, Lenexa, KS USA. [Khouryieh, H.] Western Kentucky Univ, Bowling Green, KY 42101 USA. [Aramouni, F.] Kansas State Univ, Food Sci Inst, Manhattan, KS 66506 USA. [Herald, T.] ARS, Ctr Grain & Anim Hlth Res, USDA, Manhattan, KS USA. RP Khouryieh, H (reprint author), Western Kentucky Univ, Bowling Green, KY 42101 USA. EM hanna.khouryieh@wku.edu NR 44 TC 5 Z9 5 U1 6 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0146-9428 EI 1745-4557 J9 J FOOD QUALITY JI J. Food Qual. PD APR PY 2014 VL 37 IS 2 BP 95 EP 106 DI 10.1111/jfq.12080 PG 12 WC Food Science & Technology SC Food Science & Technology GA AE9YB UT WOS:000334367100003 ER PT J AU Dominguez-Salas, P Moore, SE Baker, MS Bergen, AW Cox, SE Dyer, RA Fulford, AJ Guan, YT Laritsky, E Silver, MJ Swan, GE Zeisel, SH Innis, SM Waterland, RA Prentice, AM Hennig, BJ AF Dominguez-Salas, Paula Moore, Sophie E. Baker, Maria S. Bergen, Andrew W. Cox, Sharon E. Dyer, Roger A. Fulford, Anthony J. Guan, Yongtao Laritsky, Eleonora Silver, Matt J. Swan, Gary E. Zeisel, Steven H. Innis, Sheila M. Waterland, Robert A. Prentice, Andrew M. Hennig, Branwen J. TI Maternal nutrition at conception modulates DNA methylation of human metastable epialleles SO NATURE COMMUNICATIONS LA English DT Article ID INTRAUTERINE GROWTH RESTRICTION; SEASONALITY; HYPOTHESIS; ORIGINS; DISEASE; WOMEN; BIRTH; BIAS AB In experimental animals, maternal diet during the periconceptional period influences the establishment of DNA methylation at metastable epialleles in the offspring, with permanent phenotypic consequences. Pronounced naturally occurring seasonal differences in the diet of rural Gambian women allowed us to test this in humans. We show that significant seasonal variations in methyl-donor nutrient intake of mothers around the time of conception influence 13 relevant plasma biomarkers. The level of several of these maternal biomarkers predicts increased/decreased methylation at metastable epialleles in DNA extracted from lymphocytes and hair follicles in infants postnatally. Our results demonstrate that maternal nutritional status during early pregnancy causes persistent and systemic epigenetic changes at human metastable epialleles. C1 [Dominguez-Salas, Paula; Moore, Sophie E.; Cox, Sharon E.; Fulford, Anthony J.; Silver, Matt J.; Prentice, Andrew M.; Hennig, Branwen J.] Gambia & London Sch Hyg & Trop Med, LSHTM, EPH, MRC Int Nutr Grp,MRC Keneba, London WC1E 7HT, England. [Baker, Maria S.; Guan, Yongtao; Laritsky, Eleonora; Waterland, Robert A.] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [Bergen, Andrew W.] SRI Int, Ctr Hlth Sci, Menlo Pk, CA 94025 USA. [Dyer, Roger A.] Univ British Columbia, Dept Pediat, Vancouver, BC V5Z 4H4, Canada. [Guan, Yongtao; Waterland, Robert A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Swan, Gary E.] Stanford Univ, Sch Med, Stanford Prevent Res Ctr, Stanford, CA 94305 USA. [Zeisel, Steven H.] Univ North Carolina Chapel Hill, Inst Nutr Res, Dept Nutr, Gillings Sch Global Publ Hlth, Kannapolis, NC 28081 USA. [Zeisel, Steven H.] Univ North Carolina Chapel Hill, Sch Med, Kannapolis, NC 28081 USA. RP Waterland, RA (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM waterland@bcm.edu; branwen.hennig@lshtm.ac.uk RI Hennig, Branwen/M-6444-2014; OI Silver, Matt/0000-0002-3852-9677; Cox, Sharon/0000-0002-9908-2936; Bergen, Andrew/0000-0002-1237-7644 FU Wellcome Trust [WT086369MA]; UK Medical Research Council (MRC) [MC-A760-5QX00]; UK Department for the International Development (DFID) under the MRC/DFID; NIH/National Institute of Diabetes and Digestive and Kidney Diseases [1R01DK081557]; USDA [CRIS 6250-51000-055]; SRI's Center for Health Sciences; NIH [DA011170, DA020830] FX This study would not have been possible without the women and children of West Kiang, whom we thank for their participation. We thank our laboratory technicians, field assistants, nurses, data entry clerks and other staff at MRC Keneba, The Gambia, in particular Bakary Sonko. Thanks also to Lorna Cox and Chris Bates at MRC HNR, Cambridge. This study was supported by a Wellcome Trust grant WT086369MA (to BJH), and core funding MC-A760-5QX00 to the International Nutrition Group by the UK Medical Research Council (MRC) and the UK Department for the International Development (DFID) under the MRC/DFID Concordat agreement. R.A.W. was supported by grants from NIH/National Institute of Diabetes and Digestive and Kidney Diseases (1R01DK081557) and the USDA (CRIS 6250-51000-055). Twin pairs were recruited from the Twin Research Registry of the SRI International15 with support by funds from SRI's Center for Health Sciences and NIH grants DA011170 and DA020830. We thank the twins of the Registry for their continuing participation in and dedication to science. NR 20 TC 112 Z9 116 U1 4 U2 28 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2041-1723 J9 NAT COMMUN JI Nat. Commun. PD APR PY 2014 VL 5 AR 3746 DI 10.1038/ncomms4746 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AG2CT UT WOS:000335224100001 PM 24781383 ER PT J AU Kunde-Ramamoorthy, G Coarfa, C Laritsky, E Kessler, NJ Harris, RA Xu, MC Chen, R Shen, LL Milosavljevic, A Waterland, RA AF Kunde-Ramamoorthy, Govindarajan Coarfa, Cristian Laritsky, Eleonora Kessler, Noah J. Harris, R. Alan Xu, Mingchu Chen, Rui Shen, Lanlan Milosavljevic, Aleksandar Waterland, Robert A. TI Comparison and quantitative verification of mapping algorithms for whole-genome bisulfite sequencing SO NUCLEIC ACIDS RESEARCH LA English DT Article ID DNA METHYLATION; METHYLOME ANALYSIS; SOFTWARE; ALIGNMENT; READS; ALIGNER; PACKAGE; CELLS AB Coupling bisulfite conversion with next-generation sequencing (Bisulfite-seq) enables genome-wide measurement of DNA methylation, but poses unique challenges for mapping. However, despite a proliferation of Bisulfite-seq mapping tools, no systematic comparison of their genomic coverage and quantitative accuracy has been reported. We sequenced bisulfite-converted DNA from two tissues from each of two healthy human adults and systematically compared five widely used Bisulfite-seq mapping algorithms: Bismark, BSMAP, Pash, BatMeth and BS Seeker. We evaluated their computational speed and genomic coverage and verified their percentage methylation estimates. With the exception of BatMeth, all mappers covered >70% of CpG sites genome-wide and yielded highly concordant estimates of percentage methylation (r(2) >= 0.95). Fourfold variation in mapping time was found between BSMAP (fastest) and Pash (slowest). In each library, 8-12% of genomic regions covered by Bismark and Pash were not covered by BSMAP. An experiment using simulated reads confirmed that Pash has an exceptional ability to uniquely map reads in genomic regions of structural variation. Independent verification by bisulfite pyrosequencing generally confirmed the percentage methylation estimates by the mappers. Of these algorithms, Bismark provides an attractive combination of processing speed, genomic coverage and quantitative accuracy, whereas Pash offers considerably higher genomic coverage. C1 [Kunde-Ramamoorthy, Govindarajan; Laritsky, Eleonora; Shen, Lanlan; Waterland, Robert A.] USDA ARS, Dept Pediat, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Coarfa, Cristian] Baylor Coll Med, Dept Mol & Cell Biol, Houston, TX 77030 USA. [Kessler, Noah J.] Univ Houston, Dept Biol & Biochem, Houston, TX 77204 USA. [Harris, R. Alan; Xu, Mingchu; Chen, Rui; Milosavljevic, Aleksandar; Waterland, Robert A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. RP Waterland, RA (reprint author), USDA ARS, Dept Pediat, Baylor Coll Med, Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM waterland@bcm.edu OI Kessler, Noah/0000-0002-2740-6663 FU National Institutes of Health Roadmap Epigenomics Program [U01DA025956]; National Institutes of Health-NIDDK [1R01DK081557]; United States Department of Agriculture [CRIS 6250-51000-055] FX The National Institutes of Health Roadmap Epigenomics Program [U01DA025956 to A.M. and R.A.W.]; National Institutes of Health-NIDDK [1R01DK081557]; United States Department of Agriculture [CRIS 6250-51000-055 to R.A.W.]. Funding for open access charge: National Institutes of Health Roadmap Epigenomics Program [U01 DA025956]. NR 30 TC 16 Z9 16 U1 2 U2 18 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-1048 EI 1362-4962 J9 NUCLEIC ACIDS RES JI Nucleic Acids Res. PD APR PY 2014 VL 42 IS 6 AR e43 DI 10.1093/nar/gkt1325 PG 10 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AF5MW UT WOS:000334758600006 PM 24391148 ER PT J AU Carpenter, TE Coggins, VL McCarthy, C O'Brien, CS O'Brien, JM Schommer, TJ AF Carpenter, Tim E. Coggins, Victor L. McCarthy, Clinton O'Brien, Chans S. O'Brien, Joshua M. Schommer, Timothy J. TI A spatial risk assessment of bighorn sheep extirpation by grazing domestic sheep on public lands SO PREVENTIVE VETERINARY MEDICINE LA English DT Article DE Bighorn sheep; Epidemic simulation model; Viability analysis; Wildlife disease; Wildlife-livestock interface ID ROCKY-MOUNTAIN BIGHORN; PASTEURELLA-HAEMOLYTICA; OVIS-CANADENSIS; MANNHEIMIA-HAEMOLYTICA; PNEUMONIA EPIZOOTICS; HOME-RANGE; ARIES; POPULATIONS; SURVIVAL; SEROTYPE AB Bighorn sheep currently occupy just 30% of their historic distribution, and persist in populations less than 5% as abundant overall as their early 19th century counterparts. Present-day recovery of bighorn sheep populations is in large part limited by periodic outbreaks of respiratory disease, which can be transmitted to bighorn sheep via contact with domestic sheep grazing in their vicinity. In order to assess the viability of bighorn sheep populations on the Payette National Forest (PNF) under several alternative proposals for domestic sheep grazing, we developed a series of interlinked models. Using telemetry and habitat data, we characterized herd home ranges and foray movements of bighorn sheep from their home ranges. Combining foray model movement estimates with known domestic sheep grazing areas (allotments), a Risk of Contact Model estimated bighorn sheep contact rates with domestic sheep allotments. Finally, we used demographic and epidemiologic data to construct population and disease transmission models (Disease Model), which we used to estimate bighorn sheep persistence under each alternative grazing scenario. Depending on the probability of disease transmission following interspecies contact, extirpation probabilities for the seven bighorn sheep herds examined here ranged from 20% to 100%. The Disease Model allowed us to assess the probabilities that varied domestic sheep management scenarios would support persistent populations of free-ranging bighorn sheep. (c) 2014 Elsevier B.V. All rights reserved. C1 [O'Brien, Joshua M.] Univ Calif Davis, CADMS, Sch Vet Med, Davis, CA 95616 USA. [Carpenter, Tim E.] Massey Univ, EpiCtr, Inst Vet Anim & Biomed Sci, Palmerston North, New Zealand. [Coggins, Victor L.] Oregon Dept Fish & Wildlife, Corvallis, OR USA. [McCarthy, Clinton] US Forest Serv, Ogden, UT USA. [O'Brien, Chans S.] USDA, Forest Serv, Payette Natl Forest, McCall, ID USA. [Schommer, Timothy J.] USDA, Forest Serv, Wallowa Whitman Natl Forest, Golden, CO USA. RP Carpenter, TE (reprint author), Massey Univ, EpiCtr, Inst Vet Anim & Biomed Sci, Palmerston North, New Zealand. EM t.e.carpenter@massey.ac.nz FU United States Department of Agriculture: Forest Service FX This research was funded by the United States Department of Agriculture: Forest Service. We thank the working group convened by the PNF that included bighorn sheep biologists, wildlife disease experts, and representatives from five US National Forests, the Intermountain Regional Office of the US Forest Service, three State fish and wildlife agencies, and four Native American Tribes. NR 57 TC 5 Z9 5 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0167-5877 EI 1873-1716 J9 PREV VET MED JI Prev. Vet. Med. PD APR 1 PY 2014 VL 114 IS 1 SI SI BP 3 EP 10 DI 10.1016/j.prevetmed.2014.01.008 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA AF1PO UT WOS:000334486000002 PM 24507886 ER PT J AU Chang, S Xie, QM Dunn, JR Ernst, CW Song, JZ Zhang, HM AF Chang, Shuang Xie, Qingmei Dunn, John R. Ernst, Catherine W. Song, Jiuzhou Zhang, Huanmin TI Host genetic resistance to Marek's disease sustains protective efficacy of herpesvirus of turkey in both experimental and commercial lines of chickens SO VACCINE LA English DT Article DE Marek's disease; Host genetics; Vaccine efficacy ID B-HAPLOTYPE INFLUENCE; WHITE LEGHORN CHICKENS; RELATIVE EFFICACY; VIRUS; VACCINES; VACCINATION; EVOLUTION; INFECTION; VIRULENCE; RESPONSES AB Marek's disease (MD) remains a continual threat to the poultry industry worldwide as the MD virus continues evolving in virulence. MD has been controlled primarily by intensive use of vaccines since 1969. Based on the antigenic and pathogenic differences of the viruses that the vaccines were derived from, commercially available MD vaccines are classified into three categories, MDV-1,-2,and -3 vaccines. This study was designed to compare the protective efficacy of MDV-1 (CVI988/Rispens) and MDV-3 (HVT) vaccines against challenge of a very virulent plus strain of Marek's disease virus (vv+MDV) in experimental and commercial egg-layer lines of chickens under controlled experimental conditions. The two experimental lines (63 and 72) of chickens carry a uniform MHC B*2 haplotype and are known to differ in resistance to MD. One of the two commercial egg-layer lines (WL and BL) segregates for three MHC haplotypes (B*2, B*15, and B"21); the other is unclear. MD incidences of the unvaccinated groups of both experimental lines and both commercial lines were 100% or close to 100% induced by the vv+MDV, 648A. Survived day patterns of the unvaccinated groups significantly differed between the two experimental lines, but did not between the two commercial lines, which suggested the two experimental lines do differ in resistance to MD but not between the two commercial lines. At manufacturers' recommended vaccine dosage, two HVTs conveyed comparable protection for the MD resistant line 63 chickens as did both CVI988/Rispens used in this study. The two HVTs also conveyed comparable protection for both commercial lines of chickens as did one of two CVI988/Rispens (CVI988/Rispens-A). At a 2000 PFU uniform dose, HVT and CVI988/Rispens again conveyed comparable protection for the MD resistant experimental line of chickens. The findings suggest vaccine protective efficacy is modulated by factors including the types and the sources of vaccines and the genetic backgrounds of chickens. The findings also suggest HVT delivers equal protection in MD resistant lines of chickens as does the industry-recognized golden standard of MD vaccine, CVI988/Rispens. Published by Elsevier Ltd. C1 [Chang, Shuang; Xie, Qingmei; Dunn, John R.; Zhang, Huanmin] USDA ARS, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Chang, Shuang; Xie, Qingmei; Ernst, Catherine W.] Michigan State Univ, Dept Anim Sci, E Lansing, MI 48824 USA. [Xie, Qingmei] South China Agr Univ, Guangzhou 510642, Guangdong, Peoples R China. [Song, Jiuzhou] Univ Maryland, College Pk, MD 20742 USA. RP Zhang, HM (reprint author), USDA ARS, Avian Dis & Oncol Lab, 3606 E Mt Hope Rd, E Lansing, MI 48823 USA. EM shchang@msu.edu; qmx@scau.edu.cn; john.dunn@ars.usda.gov; ernstc@msu.edu; songj88@umd.edu; huanmin.zhang@ars.usda.gov FU USDA AFRI [2010-65205-20588]; USDA-Agriculture Research Service [3635-31320-009-04S]; Michigan State University [3635-31320-009-04S] FX The authors thank Bernice Li for her technical assistance in conducting the challenge trials. This work was supported in part by a USDA AFRI grant (2010-65205-20588) and a Specific Cooperative Agreement (3635-31320-009-04S) between USDA-Agriculture Research Service and Michigan State University. NR 44 TC 4 Z9 4 U1 0 U2 14 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0264-410X EI 1873-2518 J9 VACCINE JI Vaccine PD APR 1 PY 2014 VL 32 IS 16 BP 1820 EP 1827 DI 10.1016/j.vaccine.2014.01.092 PG 8 WC Immunology; Medicine, Research & Experimental SC Immunology; Research & Experimental Medicine GA AF1OP UT WOS:000334483500009 PM 24530405 ER PT J AU Dong, JN Ochsner, TE Zreda, M Cosh, MH Zou, CB AF Dong, Jingnuo Ochsner, Tyson E. Zreda, Marek Cosh, Michael H. Zou, Chris B. TI Calibration and Validation of the COSMOS Rover for Surface Soil Moisture Measurement SO VADOSE ZONE JOURNAL LA English DT Article AB The COsmic-ray Soil Moisture Observing System (COSMOS) rover may be useful for validating satellite-based estimates of near-surface soil moisture, but the accuracy with which the rover can measure 0- to 5-cm soil moisture has not been previously determined. Our objectives were to calibrate and validate a COSMOS rover for mapping 0- to 5-cm soil moisture at spatial scales suitable for evaluating satellite-based soil moisture estimates. Region-specific calibrations for the COSMOS rover were developed using field-average soil moisture measured using impedance probes and the oven-drying method on volumetric samples. The resulting calibrations were applied to map soil moisture on two dates in a 16- by 10-km region around the Marena, Oklahoma, In Situ Sensor Testbed (MOISST) in north central Oklahoma and one date in a 34- by 14-km region in the Little Washita River watershed in southwestern Oklahoma. The mapped soil moisture patterns were consistent with the regional spatial variability in surface soil texture and with soil wetting by an intervening rainfall. The rover measured field-average soil moisture with a root mean squared difference (RMSD) of 0.03 cm(3) cm(-3) relative to impedance probes. Likewise, the regional-average 0- to 5-cm soil moisture determined by the rover was within +/- 0.03 cm(3) cm(-3) of the best available independent estimates for each region. These results demonstrate that a COSMOS rover can be used effectively for 0- to 5-cm soil moisture mapping and for determining the average soil moisture at spatial scales suitable for satellite calibration and validation. C1 [Dong, Jingnuo; Ochsner, Tyson E.; Zou, Chris B.] Oklahoma State Univ, Stillwater, OK 74078 USA. [Zreda, Marek] Univ Arizona, Tucson, AZ 85721 USA. [Cosh, Michael H.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. RP Ochsner, TE (reprint author), Oklahoma State Univ, Stillwater, OK 74078 USA. EM tyson.ochsner@okstate.edu RI Cosh, MIchael/A-8858-2015; Zou, Chris/A-5039-2010 OI Cosh, MIchael/0000-0003-4776-1918; Zou, Chris/0000-0003-0080-2866 FU USDA-ARS; Oklahoma Agricultural Experiment Station; U.S. National Science Foundation EPSCoR program [131789]; U.S. National Science Foundation [ATM-0838491] FX The contributions of Jingnuo Dong and Michael Cosh were funded by the USDA-ARS. The contributions of Tyson Ochsner and Chris Zou were funded by the Oklahoma Agricultural Experiment Station and by the U.S. National Science Foundation EPSCoR program (Grant no. 131789). The contribution of Marek Zreda was funded through the COSMOS project by the U.S. National Science Foundation (Grant no. ATM-0838491). We thank the anonymous reviewers and the associate editor for their valuable assistance in improving this manuscript. NR 27 TC 17 Z9 17 U1 0 U2 21 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD APR PY 2014 VL 13 IS 4 DI 10.2136/vzj2013.08.0148 PG 8 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AF7WZ UT WOS:000334927400002 ER PT J AU Flerchinger, GN Seyfried, MS AF Flerchinger, G. N. Seyfried, M. S. TI Comparison of Methods for Estimating Evapotranspiration in a Small Rangeland Catchment SO VADOSE ZONE JOURNAL LA English DT Article ID BOREAL ASPEN FOREST; WATER-VAPOR FLUXES; ENERGY-BALANCE; CARBON-DIOXIDE; UNITED-STATES; HEAT FLUXES; SHAW MODEL; EXCHANGE; CANOPY; TEMPERATURE AB Evapotranspiration (ET) was quantified for two rangeland vegetation types, aspen and sagebrush-grassland, over an 8-yr study period by comparing the following approaches for estimating ET: eddy covariance systems (EC, available for only 6 yr); soil water storage loss measured by time domain reflectometry (TDR) and neutron probe; and model simulation. The research site, the Upper Sheep Creek catchment in the Reynolds Creek Experimental Watershed, is part of a study on the effects of prescribed fire and vegetation removal. Estimates of seasonal ET for the aspen using EC with the turbulent fluxes adjusted to force energy balance closure, a 180-cm TDR soil profile, two 225-cm neutron access tubes, and the Simultaneous Heat and Water (SHAW) model agreed well with each other. If the two neutron probes were averaged, the RMSD of all approaches over the 6 yr was within 8% of the average. For the sagebrush-grassland, a 120-cm TDR profile underestimated seasonal ET for all years except the year immediately following the prescribed fire, when rooting depth likely had not recovered. A 195-cm neutron probe access tube located within 30 m of the stream underestimated ET for every year except when there was no streamflow, suggesting that lateral flow may have biased the results for this tube. A comparison of the other methods (EC flux adjusted to force energy balance closure, soil water loss measured from a 225-cm neutron access tube, and SHAW model simulation) agreed within 3% during the 6 yr with EC measurements at that site. C1 [Flerchinger, G. N.; Seyfried, M. S.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. RP Flerchinger, GN (reprint author), USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. EM gerald.flerchinger@ars.usda.gov NR 46 TC 5 Z9 5 U1 1 U2 21 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD APR PY 2014 VL 13 IS 4 DI 10.2136/vzj2013.08.0152 PG 11 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AF7WZ UT WOS:000334927400003 ER PT J AU Deaton, MK Spear, A Faaberg, KS Pegan, SD AF Deaton, Michelle K. Spear, Allyn Faaberg, Kay S. Pegan, Scott D. TI The vOTU domain of highly-pathogenic porcine reproductive and respiratory syndrome virus displays a differential substrate preference SO VIROLOGY LA English DT Article DE vOTU; PLP2; PRRSV; nsp2; Ubiquitin; ISG15 ID CYSTEINE PROTEASE DOMAIN; INNATE IMMUNE-RESPONSE; KAPPA-B ACTIVATION; NONSTRUCTURAL PROTEIN-2; RIG-I; MOLECULAR VARIATION; PAPAIN-LIKE; UBIQUITIN; ARTERIVIRUS; STRAINS AB Arterivirus genus member Porcine reproductive and respiratory syndrome virus (PRRSV) causes an economically devastating disease, recently exacerbated by the emergence of highly pathogenic strains (HP-PRRSV). Within the nonstructural protein 2 of PRRSV is a deubiquitinating enzyme domain belonging to the viral ovarian tumor (vOTU) protease superfamily. vOTUs, which can greatly vary in their preference for their host ubiquitin (Ub) and Ub-like substrates such as interferon stimulated gene 15 (ISG15), have been implicated as a potential virulence factor. Since various strains of PRRSV have large variations in virulence, the specificity of vOTUs from two PRRSV strains of varying virulence were determined. While both vOTUs showed de-ubiquitinating activity and markedly low deISGylating activity, HP-PRRSV demonstrated a strong preference for lysine 63-linked poly-Ubiquitin, tied to innate immune response regulation. This represents the first report of biochemical activity unique to HP-PRRSV that has implications for a potential increase in immunosuppression and virulence. (C) 2014 Elsevier Inc. All rights reserved. C1 [Deaton, Michelle K.] Univ Denver, Dept Chem & Biochem, Denver, CO 80208 USA. [Spear, Allyn; Faaberg, Kay S.] USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50010 USA. [Pegan, Scott D.] Univ Georgia, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA. RP Pegan, SD (reprint author), Univ Georgia, Dept Pharmaceut & Biomed Sci, Athens, GA 30602 USA. EM spegan@uga.edu FU NIH [1R01AI109008-01, 1R03AI092249-01] FX This work was funding in part by NIH 1R01AI109008-01 (SDP) and 1R03AI092249-01 (SDP). NR 54 TC 3 Z9 3 U1 0 U2 8 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0042-6822 J9 VIROLOGY JI Virology PD APR PY 2014 VL 454 BP 247 EP 253 DI 10.1016/j.virol.2014.02.026 PG 7 WC Virology SC Virology GA AF4AY UT WOS:000334655000026 PM 24725951 ER PT J AU Dai, ZH Birdsey, RA Johnson, KD Dupuy, JM Hernandez-Stefanoni, JL Richardson, K AF Dai, Zhaohua Birdsey, Richard A. Johnson, Kristofer D. Manuel Dupuy, Juan Luis Hernandez-Stefanoni, Jose Richardson, Karen TI Modeling Carbon Stocks in a Secondary Tropical Dry Forest in the Yucatan Peninsula, Mexico SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Biomass; Forest-DNDC; Greenhouse gas; Disturbance; Tropical dry forest ID SEMI-DECIDUOUS FOREST; PROCESS-ORIENTED MODEL; METHANE UPTAKE; MAYA CIVILIZATION; TEMPERATE FOREST; NO EMISSIONS; CLIMATE; SOILS; DYNAMICS; BIOMASS AB The carbon balance of secondary dry tropical forests of Mexico's Yucatan Peninsula is sensitive to human and natural disturbances and climate change. The spatially explicit process model Forest-DeNitrificationDeComposition (DNDC) was used to estimate forest carbon dynamics in this region, including the effects of disturbance on carbon stocks. Model evaluation using observations from 276 sample plots in a tropical dry forest in the Yucatan Peninsula indicated that Forest-DNDC can be used to simulate carbon stocks for this forest with good model performance efficiency. The simulated spatial variability in carbon stocks was large, ranging from 5 to 115 Mg carbon (C) ha(-1), with a mean of 56.6 Mg C ha(-1). Carbon stocks in the forest were largely influenced by human disturbances between 1985 and 2010. Based on a comparison of the simulations with and without disturbances, carbon storage in the year 2012 with disturbance was 3.2 Mg C ha(-1), lower on average than without disturbance. The difference over the whole study area was 154.7 Gg C, or an 8.5 % decrease. There were substantial differences in carbon stocks simulated at individual sample plots, compared to spatially modeled outputs (200 m(2) plots vs. polygon simulation units) at some locations due to differences in vegetation class, stand age, and soil conditions at different resolutions. However, the difference in the regional mean of carbon stocks between plot-level simulation and spatial output was small. Soil CO2 and N2O fluxes varied spatially; both fluxes increased with increasing precipitation, and soil CO2 also increased with an increase in biomass. The modeled spatial variability in CH4 uptake by soils was small, and the flux was not correlated with precipitation. The net ecosystem exchange (NEE) and net primary production (NPP) were nonlinearly correlated with stand age. Similar to the carbon stock simulations, different resolutions resulted in some differences in NEE and NPP, but the spatial means were similar. C1 [Dai, Zhaohua; Birdsey, Richard A.; Johnson, Kristofer D.] US Forest Serv, USDA, Newtown Sq, PA 19073 USA. [Dai, Zhaohua; Richardson, Karen] Commiss Environm Cooperat North Amer, Montreal, PQ H2Y 1N9, Canada. [Manuel Dupuy, Juan; Luis Hernandez-Stefanoni, Jose] Ctr Invest Cient Yucatan AC, Unidad Recursos Nat, Merida 97200, Yucatan, Mexico. RP Dai, ZH (reprint author), US Forest Serv, USDA, 11 Campus Blvd,Suite 200, Newtown Sq, PA 19073 USA. EM zdai@fs.fed.us OI Hernandez-Stefanoni, Jose Luis/0000-0002-9559-7131 FU North American Commission for Environmental Cooperation; US Agency for International Development FX This project was supported by the North American Commission for Environmental Cooperation and the US Agency for International Development. This research was conducted with data from the Mexican network of Intensive Carbon Monitoring Sites (MEX-SMIC). The network is an affiliation of Academic Institutions, Government and NGO investigators with various funding sources. The network currently includes five forested sites located in strategic landscapes for MRV-REDD + activities. Ecosystem C has been estimated using similar protocols since 2012 at various spatial and temporal scales with a variety of methods (e.g., inventory plots, remote sensing, eddy covariance, and ecosystem carbon models). More details can be found at: http://www.mrv.mx/mexsmic. We thank Dr. Jeff Masek of NASA for providing the disturbance map. NR 63 TC 3 Z9 4 U1 6 U2 50 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD APR PY 2014 VL 225 IS 4 AR 1925 DI 10.1007/s11270-014-1925-x PG 15 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA AF2YL UT WOS:000334578300016 ER PT J AU Nair, SS Zhu, JY Deng, YL Ragauskas, AJ AF Nair, Sandeep S. Zhu, J. Y. Deng, Yulin Ragauskas, Arthur J. TI Hydrogels Prepared from Cross-Linked Nanofibrillated Cellulose SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Nanofibrillated cellulose; Hydrogels; Water absorption; Gel content; Thermal stability; Tensile property ID NANOCOMPOSITE HYDROGELS; POLY(ETHYLENE GLYCOL); MECHANICAL-PROPERTIES; NETWORK STRUCTURE; NANO; POLY(N-ISOPROPYLACRYLAMIDE); NANOWHISKERS; COMPOSITES; FIBRILS; DESIGN AB Nanocomposite hydrogels were developed by cross-linking nanofibrillated cellulose with poly(methyl vinyl ether-co-maleic acid) and polyethylene glycol. The cross-linked hydrogels showed enhanced water absorption and gel content with the addition of nanocellulose. In addition, the thermal stability, mechanical strength, and modulus increased with an increase in the amount of nanocellulose in hydrogels, and this can be attributed to efficient cross-linking between the nanocellulose and the matrix. The addition of softwood nanocellulose showed much higher strength and strain properties in the hydrogels than with the addition of hardwood nano cellulose. The enhanced physical properties confirm that in situ cross-linking of nanofibrillated cellulose with the matrix polymer forms hydrogels that are not just blends of starting materials but are distinctively unique and formed by cross-linking interactions between the filler and matrix. C1 [Nair, Sandeep S.; Ragauskas, Arthur J.] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA. [Deng, Yulin] Georgia Inst Technol, Sch Chem & Biomol Engn, Atlanta, GA 30332 USA. [Zhu, J. Y.] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA. RP Ragauskas, AJ (reprint author), Georgia Inst Technol, Sch Chem & Biochem, 500 10th St NW, Atlanta, GA 30332 USA. EM arthur.ragauskas@chemistry.gatech.edu OI Ragauskas, Arthur/0000-0002-3536-554X FU USDA Forest Service RD FX This work was partially supported by the USDA Forest Service R&D special funding on Cellulose Nano-Materials (2012). NR 31 TC 17 Z9 19 U1 7 U2 73 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD APR PY 2014 VL 2 IS 4 BP 772 EP 780 DI 10.1021/sc400445t PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA AE6IA UT WOS:000334092600029 ER PT J AU Mohammad, MA Sunehag, AL Haymond, MW AF Mohammad, Mahmoud A. Sunehag, Agneta L. Haymond, Morey W. TI De novo synthesis of milk triglycerides in humans SO AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM LA English DT Article DE fatty acid; glycerol; GC-MS; gene expression; lipogenesis; stable isotopes ID FATTY-ACID SYNTHESIS; MEDIUM-CHAIN TRIGLYCERIDES; HUMAN MAMMARY EPITHELIUM; ACETYL-COA CARBOXYLASE; POOL IN-VIVO; GENE-EXPRESSION; PREMATURE-INFANTS; LIPID-SYNTHESIS; LACTATING RATS; HUMAN-BREAST AB Mammary gland (MG) de novo lipogenesis contributes significantly to milk fat in animals but little is known in humans. Objective: To test the hypothesis that the incorporation of C-13 carbons from [U-C-13] glucose into fatty acids (FA) and glycerol in triglycerides (TG) will be greater: 1) in milk than plasma TG, 2) during a high-carbohydrate (H-CHO) diet than high-fat (H-FAT) diet, and 3) during feeding than fasting. Seven healthy, lactating women were studied on two isocaloric, isonitrogenous diets. On one occasion, subjects received diets containing H-FAT or H-CHO diet for 1 wk. Incorporation of C-13 from infused [U-C-13] glucose into FA and glycerol was measured using GC-MS and gene expression in RNA isolated from milk fat globule using microarrays. Incorporation of C-13(2) into milk FA increased with increased FA chain length from C2: 0 to C12: 0 but progressively declined in C14: 0 and C16: 0 and was not detected in FA > C16. During feeding, regardless of diets, enrichment of 13C2 in milk FA and C-13(3) in milk glycerol were similar to 3-and similar to 7-fold higher compared with plasma FA and glycerol, respectively. Following an overnight fast during H-CHO and H-FAT diets, 25 and 6%, respectively, of medium-chain FA (MCFA, C6-C12) in milk were derived from glucose but increased to 75 and 25% with feeding. Expression of genes involved in FA or glycerol synthesis was unchanged regardless of diet or fast/fed conditions. The human MG is capable of de novo lipogenesis of primarily MCFA and glycerol, which is influenced by the macronutrient composition of the maternal diet. C1 [Mohammad, Mahmoud A.; Sunehag, Agneta L.; Haymond, Morey W.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX USA. RP Haymond, MW (reprint author), Childrens Nutr Res Ctr, 1100 Bates St, Houston, TX 77030 USA. EM mhaymond@bcm.edu OI Mohammad, Mahmoud/0000-0002-6535-5529 FU NIH [RO1 DK-55478, HD-37857, MO1 RR-00188, USDA/ARS 6250-5100] FX This project was supported by NIH Grants RO1 DK-55478, HD-37857, MO1 RR-00188, USDA/ARS 6250-5100. This work is a publication of the USDA/ARS Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine (Houston, TX). The contents of this publication do not necessarily reflect the views of policies of the US Dept. of Agriculture, nor does mention of trade names, commercial products, or organizations imply endorsement from the US Government. NR 48 TC 1 Z9 1 U1 1 U2 13 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1849 EI 1522-1555 J9 AM J PHYSIOL-ENDOC M JI Am. J. Physiol.-Endocrinol. Metab. PD APR PY 2014 VL 306 IS 7 BP E838 EP E847 DI 10.1152/ajpendo.00605.2013 PG 10 WC Endocrinology & Metabolism; Physiology SC Endocrinology & Metabolism; Physiology GA AE9TX UT WOS:000334354300013 PM 24496312 ER PT J AU Thornton, MK Novy, RG Stark, JC AF Thornton, Michael K. Novy, Richard G. Stark, Jeffery C. TI Improving Phosphorus Use Efficiency in the Future SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article; Proceedings Paper CT 96th Annual Meeting of the Potato-Association-of-America on Potato Phosphorus Management and Utilization for Today and Tomorrow CY AUG 13, 2012 CL Denver, CO SP Potato Assoc Amer, Prod & Management Sect DE Uptake; Utilization; Root health; Genetic improvement ID RUSSET BURBANK POTATO; ROOT-GROWTH; NITROGEN; PLANTS; REQUIREMENTS; CULTIVARS; SYSTEM; SOILS; CROP AB World demand for phosphorus (P) fertilizer and increasing environmental regulations concerning water quality will continue to motivate the search for ways to improve P use efficiency in potato production. While much effort has gone into understanding P availability and uptake from soil, relatively little is known about the possibility of increasing uptake efficiency through genetic improvement or improved root health. Potato exhibits considerable genetic variation in uptake efficiency of nutrients like nitrogen, and it is likely that genetic variation also exists in P use efficiency. Increased efficiency may be due to differences in tuber yield per unit of available P (utilization efficiency), or in rate of P absorption from the soil (uptake efficiency). Likewise, genetic improvements in resistance to root-decaying pathogens, such as Verticillium dahliae, and improved control practices are likely to help maintain a healthy root mass to aid in maintaining P uptake during periods of higher P demand during the growing season. C1 [Thornton, Michael K.] Univ Idaho, Dept Plant Soil & Entomol Sci, Parma, ID 83660 USA. [Novy, Richard G.] ARS, USDA, Aberdeen, ID 83210 USA. [Stark, Jeffery C.] Univ Idaho, Dept Plant Soil & Entomol Sci, Idaho Falls, ID 83402 USA. RP Thornton, MK (reprint author), Univ Idaho, Dept Plant Soil & Entomol Sci, Parma, ID 83660 USA. EM miket@uidaho.edu NR 29 TC 5 Z9 6 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD APR PY 2014 VL 91 IS 2 BP 175 EP 179 DI 10.1007/s12230-014-9369-9 PG 5 WC Agronomy SC Agriculture GA AE8RV UT WOS:000334270200006 ER PT J AU Brown, CR Zhang, LH Mojtahedi, H AF Brown, Charles R. Zhang, Linhai Mojtahedi, Hassan TI Tracking the R (Mc1) Gene for Resistance to Race 1 of Columbia Root-Knot Nematode (Meloidogyne chitwoodi) in Three Mexican Wild Potato Species with Different Ploidies SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article; Proceedings Paper CT 96th Annual Meeting of the Potato-Association-of-America on Potato Phosphorus Management and Utilization for Today and Tomorrow CY AUG 13, 2012 CL Denver, CO SP Potato Assoc Amer, Prod & Management Sect DE Polyploidy; Nematode resistance; Marker assisted selection (MAS); Homeologous genes ID IN-SITU HYBRIDIZATION; SOLANUM-BULBOCASTANUM; INTROGRESSED RESISTANCE; CULTIVATED POTATO; BREEDING LINES; HAPLA; HOMOLOGS; NORTH; TESTS; TUBER AB Resistance to Columbia Root-knot nematode was discovered in three wild species in a polyploidy series, Solanum bulbocastanum (2n = 2x), .S. fendleri (2n = 4x), and S. hougasii, 2n = 6x, respectively. After several generations of introgression into the cultivated gene pool, a single dominant monogene conditioning resistance to root propagation of race 1 of CRKN was theorized to be acting in breeding lines derived from each species. Coincidently, sequence tagged sites (STS) and cleaved amplified polymorphic site (CAPS) markers that associated with resistance with S. bulbocastanum were found to be informative in other segregating breeding populations regardless of the source of the gene. The utility of the markers across ploidies suggests that the original gene and genome of S. bulbocastanum or a modern derivative, is present in S. fendleri and S. hougasii. In this polyploidy series, knowledge that the S. bulbocastanum genome is the original source of useful genes guides future germplasm surveyors to search first and most intensively in the primitive Mexican diploid species that may have served as the contributor of a genome to descendants harboring this genome at higher ploidies. C1 [Brown, Charles R.] ARS, USDA, Washington, DC 20003 USA. [Zhang, Linhai; Mojtahedi, Hassan] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Brown, CR (reprint author), ARS, USDA, Washington, DC 20003 USA. EM chuck.brown@ars.usda.gov OI Zhang, Linhai/0000-0003-1009-6870 NR 27 TC 5 Z9 5 U1 2 U2 5 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD APR PY 2014 VL 91 IS 2 BP 180 EP 185 DI 10.1007/s12230-013-9336-x PG 6 WC Agronomy SC Agriculture GA AE8RV UT WOS:000334270200007 ER PT J AU Dong, JX Xie, X Hu, DW Chen, SC He, YS Beier, RC Shen, YD Sun, YM Xu, ZL Wang, H Yang, JY AF Dong, Jie-Xian Xie, Xi Hu, Da-Wei Chen, Shu-Chi He, Yong-Sheng Beier, Ross C. Shen, Yu-Dong Sun, Yuan-Ming Xu, Zhen-Lin Wang, Hong Yang, Jin-Yi TI Codon modification for the DNA sequence of a single-chain Fv antibody against clenbuterol and expression in Pichia pastoris SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Single-chain variable fragment; Clenbuterol; Codon optimization; Expression Pichia pastoris ID HUMAN-PAPILLOMAVIRUS TYPE-16; RECOMBINANT FAB ANTIBODIES; METHYLOTROPHIC YEAST; SACCHAROMYCES-CEREVISIAE; PROTEIN EXPRESSION; GENE-EXPRESSION; OPTIMIZATION; LEVEL; PURIFICATION; SELECTION AB The expression efficiency was improved for the recombinant single-chain variable fragment (scFv) against clenbuterol (CBL) obtained from mouse and expressed in the methylotrophic yeast Pichia pastoris GS115, by redesigning and synthesizing the DNA sequence encoding for CBL-scFv based on the codon bias of P. pastoris. The codons enco4ding 124 amino acids were optimized, in which a total of 156 nucleotides were changed, and the G+C ratio was simultaneously decreased from 53 to 47.2 %. Under the optimized expression conditions, the yield of the recombinant CBL-scFv (41 kDa) antibodies was 0.223 g L-1 in shake culture. Compared to the non-optimized control, the expression level of the optimized recombinant CBL-scFv based on preferred codons in P. pastoris demonstrated a 2.35-fold higher yield. Furthermore, the recombinant CBL-scFv was purified by Ni-NTA column chromatography, and the purity was 95 %. The purified CBL-scFv showed good CBL recognition by a competitive indirect enzyme-linked immunoassay. The average concentration required for 50 % inhibition of binding and the limit of detection for the assay were 5.82 and 0.77 ng mL(-1), respectively. C1 [Dong, Jie-Xian; Xie, Xi; Chen, Shu-Chi; He, Yong-Sheng; Shen, Yu-Dong; Sun, Yuan-Ming; Xu, Zhen-Lin; Wang, Hong; Yang, Jin-Yi] South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Coll Food Sci, Guangzhou 510642, Guangdong, Peoples R China. [Hu, Da-Wei] Entry Exit Inspect & Quarantine Zengcheng, Zengcheng 511340, Guangdong, Peoples R China. [Chen, Shu-Chi] Shantou Environm Monitoring Ctr, Shantou 515041, Guangdong, Peoples R China. [He, Yong-Sheng] Shenzhen Acad Metrol & Qual Inspect, Shenzhen 518055, Guangdong, Peoples R China. [Beier, Ross C.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. RP Wang, H (reprint author), South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Coll Food Sci, Guangzhou 510642, Guangdong, Peoples R China. EM gzwhongd@163.com; yjy361@163.com FU National Natural Science Foundation of China [30871755, 31271866]; Science and Technology Plan Projects in Guangdong Province [2012A020100002, 2010A032000001-4]; Hopson Zhujiang Education Fund [H2011001] FX J.-X. Dong and X. Xie contributed equally to this work. This work was supported by the National Natural Science Foundation of China (Grant 30871755, 31271866), Science and Technology Plan Projects in Guangdong Province (2012A020100002, 2010A032000001-4), and the Doctoral Innovation Program of Hopson Zhujiang Education Fund (H2011001). NR 59 TC 0 Z9 0 U1 3 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD APR PY 2014 VL 98 IS 8 BP 3679 EP 3689 DI 10.1007/s00253-013-5324-9 PG 11 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AE7IO UT WOS:000334171100026 PM 24190495 ER PT J AU Nelson, LA Davies, KA Scheffer, SJ Taylor, GS Purcell, MF Giblin-Davis, RM Thornhill, AH Yeates, DK AF Nelson, Leigh A. Davies, Kerrie A. Scheffer, Sonja J. Taylor, Gary S. Purcell, Matthew F. Giblin-Davis, Robin M. Thornhill, Andrew H. Yeates, David K. TI An emerging example of tritrophic coevolution between flies (Diptera: Fergusoninidae) and nematodes (Nematoda: Neotylenchidae) on Myrtaceae host plants SO BIOLOGICAL JOURNAL OF THE LINNEAN SOCIETY LA English DT Review DE cospeciation; COI; morphology; tritrophic interaction; tritrophic specialization ID CONTORTYLENCHUS-GRANDICOLLI NEMATODA; MELALEUCA-QUINQUENERVIA; BUD GALLS; BIOLOGICAL-CONTROL; PHYLOGENETIC-RELATIONSHIPS; FERGUSOBIA-QUINQUENERVIAE; TYLENCHIDA NEOTYLENCHIDAE; MOLECULAR PHYLOGENY; EASTERN AUSTRALIA; LIFE-HISTORY AB A unique obligate mutualism occurs between species of Fergusonina Malloch flies (Diptera: Fergusoninidae) and nematodes of the genus Fergusobia Currie (Nematoda: Neotylenchidae). These mutualists together form different types of galls on Myrtaceae, mainly in Australia. The galling association is species-specific, and each mutualism in turn displays host specificity. This tritrophic system represents a compelling arena to test hypotheses about coevolution between the host plants, parasitic nematodes and the fergusoninid flies, and the evolution of these intimate mutualisms. We have a basic knowledge of the interactions between the host plant, fly and nematode in this system, but a more sophisticated understanding will require a much more intensive and coordinated research effort. Summaries of the known Fergusonina/Fergusobia species associations and gall type terminology are presented. This paper identifies the key advantages of the system and questions to be addressed, and proposes a number of predictions about the evolutionary dynamics of the system given our understanding of the biology of the mutualists. Future research will profitably focus on (1) gall cecidogenesis and phenology, (2) the interaction between the fly larva and the nematode in the gall, and between the adult female fly and the parasitic nematode, (3) the means by which the fly and nematode life cycles are coordinated, (4) a targeted search of groups in the plant family Myrtaceae that have not yet been identified as gall hosts, and (5) establishment and comparison of the phylogenetic relationships of the host plants, fly species and nematodes. Recently derived phylogenies and divergence time estimation studies of the Diptera and the Myrtaceae show that the fly family Fergusoninidae is less than half the age of the Myrtaceae, discounting the hypothesis of cospeciation and coradiation of the fly/nematode mutualism and the plants at the broadest levels. However, cospeciation may have occurred at shallower levels in the phylogeny, following the establishment of the fly/nematode mutualism on the Myrtaceae. (c) 2014 The Linnean Society of London, Biological Journal of the Linnean Society, 2014, 111, 699-718. C1 [Nelson, Leigh A.; Yeates, David K.] CSIRO Ecosyst Sci, Acton, ACT 2601, Australia. [Davies, Kerrie A.] Univ Adelaide, Australian Ctr Evolutionary Biol & Biodivers, Osmond, SA 5064, Australia. [Davies, Kerrie A.] Univ Adelaide, Sch Agr Food & Wine, Osmond, SA 5064, Australia. [Scheffer, Sonja J.] USDA ARS, Systemat Entomol Lab, Beltsville, MD 20705 USA. [Taylor, Gary S.] Univ Adelaide, Australian Ctr Evolutionary Biol & Biodivers, Adelaide, SA 5005, Australia. [Taylor, Gary S.] Univ Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia. [Purcell, Matthew F.] CSIRO Ecosyst Sci, USDA ARS Australian Biol Control Lab, Brisbane, Qld 4001, Australia. [Giblin-Davis, Robin M.] Univ Florida, Ft Lauderdale Res & Educ Ctr, Ft Lauderdale, FL 33314 USA. [Thornhill, Andrew H.] James Cook Univ, Australian Trop Herbarium, Cairns, Qld 4870, Australia. RP Davies, KA (reprint author), Univ Adelaide, Australian Ctr Evolutionary Biol & Biodivers, Waite Campus,PMB 1, Osmond, SA 5064, Australia. EM kerrie.davies@adelaide.edu.au RI Thornhill, Andrew/I-3363-2012; Purcell, Matthew/C-2098-2008; Yeates, David/A-9917-2008 OI Thornhill, Andrew/0000-0002-0325-5725; Yeates, David/0000-0001-7729-6143 FU USDA Special Grants in Tropical and Subtropical Agriculture [CRSR-99-34135-8478, CRSR-03-34135-14078]; Australian Biological Resources Studies grants FX Special thanks to Ted and Barb Center, Paul Pratt, Greg Wheeler, Scott Blackwood, Phil Tipping, Susan Wright, Weimin Ye and Dorota Porazinska among many others at the USDA IPRL (Invasive Plant Research Lab) and the University of Florida, IFAS FLREC in Fort Lauderdale, Florida, for their help and support during studies of this amazing group of flies and nematodes for deployment as a biological control agent against M. quinquenervia in the United States. Some of the research summarized in this article was supported by USDA Special Grants in Tropical and Subtropical Agriculture (CRSR-99-34135-8478 and CRSR-03-34135-14078) to R. G. D.; and by Australian Biological Resources Studies grants to K.A.D. NR 111 TC 7 Z9 7 U1 3 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0024-4066 EI 1095-8312 J9 BIOL J LINN SOC JI Biol. J. Linnean Soc. PD APR PY 2014 VL 111 IS 4 BP 699 EP 718 DI 10.1111/bij.12237 PG 20 WC Evolutionary Biology SC Evolutionary Biology GA AD9QH UT WOS:000333599100001 ER PT J AU Bevins, SN Pedersen, K Lutman, MW Gidlewski, T Deliberto, TJ AF Bevins, Sarah N. Pedersen, Kerri Lutman, Mark W. Gidlewski, Thomas Deliberto, Thomas J. TI Consequences Associated with the Recent Range Expansion of Nonnative Feral Swine SO BIOSCIENCE LA English DT Article DE invasive species; infectious diseases; emerging diseases; conservation; ecology ID BOAR SUS-SCROFA; EUROPEAN WILD BOAR; MALAYSIAN RAIN-FOREST; UNITED-STATES; PIGS; MANAGEMENT; CONSERVATION; POPULATION; PREDATION; IMPACTS AB Feral swine (Sus scrofa) have been repeatedly introduced to locations around the world. Aided by both an adaptable biology and deliberate introductions by people, the range of invasive feral swine in the United States has expanded from 17 to 38 states over the past 30 years. The swine's generalist diet combined with high population densities can complicate efforts to conserve threatened and endangered species, and losses from crop damage and livestock predation in the United States alone are estimated to be more than $1 billion. In addition, feral swine can be a reservoir for multiple pathogens, some of which are zoonotic. Management responses to mitigate these threats by reducing population numbers face resistance from groups that value feral swine for subsistence or sport hunting, which results in complicated policy actions that are extremely divisive and difficult to implement. C1 [Bevins, Sarah N.; Pedersen, Kerri; Lutman, Mark W.; Gidlewski, Thomas; Deliberto, Thomas J.] Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Bevins, SN (reprint author), Anim & Plant Hlth Inspect Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. EM sarah.n.bevins@aphis.usda.gov NR 60 TC 19 Z9 19 U1 3 U2 65 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0006-3568 EI 1525-3244 J9 BIOSCIENCE JI Bioscience PD APR PY 2014 VL 64 IS 4 BP 291 EP 299 DI 10.1093/biosci/biu015 PG 9 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AE7AA UT WOS:000334147900008 ER PT J AU Pukazhenthi, BS Johnson, A Guthrie, HD Songsasen, N Padilla, LR Wolfe, BA da Silva, MC Alvarenga, MA Wildt, DE AF Pukazhenthi, Budhan S. Johnson, Aime Guthrie, H. David Songsasen, Nucharin Padilla, Luis R. Wolfe, Barbara A. da Silva, Marco Coutinho Alvarenga, Marco A. Wildt, David E. TI Improved sperm cryosurvival in diluents containing amides versus glycerol in the Przewalski's. horse (Equus ferus przewalskii) SO CRYOBIOLOGY LA English DT Article DE Equid; Spermatozoa; Semen; Cryoprotectant; Freezing; Mitochondria ID PHASCOLARCTOS-CINEREUS SPERMATOZOA; STALLION SPERMATOZOA; FROZEN-SEMEN; MITOCHONDRIAL-FUNCTION; COLLECTION TECHNIQUES; MEMBRANE INTEGRITY; RABBIT SPERMATOZOA; FLOW-CYTOMETRY; CRYOPRESERVATION; CRYOPROTECTANTS AB Two studies were conducted to understand sperm cryosensitivity in an endangered equid, the Przewalski's horse (Equus ferns przewalski), while testing the cryoprotectant ability of formamides. The first assessed the toxicity of permeating cryoprotectants (glycerol, methylformamide IMF] and dimethylformamide [DMF]) to Przewalski's horse spermatozoa during liquid storage at 4 C. The second examined the comparative influence of three diluents (with or without formamides) on cryosurvival of sperm from the Przewalski's versus domestic horse. When Przewalski's horse spermatozoa were incubated at 4 C in INRA 96 with differing concentrations of glycerol, MF or DMF or a combination of these amides, cells tolerated all but the highest concentration (10% v/v) of MF alone or in combination with DMF, both of which decreased (P < 0.05) motility traits. There was no effect of cryoprotectants on sperm acrosomal integrity. In the cryosurvival study, average sperm motility and proportion of cells with intact acrosomes in fresh ejaculates were similar (P> 0.05) between the Przewalski's (67%, 84%, respectively) and domestic (66%, 76%) horse donors. Sperm from both species were diluted in lactose-EDTA-glycerol (EQ), Botu-Crio (BOTU; a proprietary product containing glycerol and MF) or SM (INRA 96 plus 2% [v/v] egg yolk and 2.5% [v/v] MF and DMF) and then frozen over liquid nitrogen vapor. After thawing, the highest values recovered for total and progressive sperm motility, acrosomal integrity and mitochondria] membrane potential were 42.4%, 21.8%, 88.7% and 25.4 CN (CN = mean JC-1 fluorescence intensity/cell on a channel number scale), respectively, in the Przewalski's and 49.3%, 24.6%, 88.9% and 25.8 CN, respectively, in the domestic horse. Although sperm progressive motility and acrosome integrity did not differ (P> 0.05) among treatments across species, mitochondrial membrane potential was higher (P< 0.05) in both species using EQ compared to BOTU or SM media. Additionally, Przewalski's stallion sperm expressed higher (P < 0.05) post-thaw total motility in BOTU and SM compared to EQ whereas there were no differences among freezing diluents in the domestic horse. In summary, Przewalski's stallion sperm benefit from exposure to either MF or DMF as an alternative cryoprotectant to glycerol. Overt sperm quality appears similar between the Przewalski's and domestic horse, although the total motility of cells from the former appears more sensitive to certain freezing diluents. Nonetheless, post-thaw motility and acrosomal integrity values for Przewalski's horse spermatozoa mimic findings in the domestic horse in the presence of INRA 96 supplemented with 2% (v/v) egg yolk and a combined 2.5% concentration of MF and DMF. Published by Elsevier Inc. C1 [Pukazhenthi, Budhan S.; Songsasen, Nucharin; Padilla, Luis R.; Wildt, David E.] Natl Zool Pk, Smithsonian Conservat Biol Inst, Front Royal, VA 22630 USA. [Johnson, Aime] Auburn Univ, Coll Vet Med, Dept Clin Sci, Auburn, AL 36849 USA. [Guthrie, H. David] ARS, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Wolfe, Barbara A.] The Wilds, Cumberland, OH 43732 USA. [da Silva, Marco Coutinho] Ohio State Univ, Coll Vet Med, Dept Vet Clin Sci, Columbus, OH 43210 USA. [Alvarenga, Marco A.] Sao Paulo State Univ, UNESP, Sch Vet Med, Dept Anim Reprod & Vet Radiol, Sao Paulo, Brazil. RP Pukazhenthi, BS (reprint author), Natl Zool Pk, Smithsonian Conservat Biol Inst, Front Royal, VA 22630 USA. EM pukazhenthib@si.edu; akj0001@auburn.edu; dave.guthrie@ars.usda.gov; songsasenn@si.edu; padilla@stlzoo.org; Barbara.wolfe@cvm.osu.edu; Marco.CoutinhoDaSilva@cvm.osu.edu; malvarenga@fmvz.unesp.br; wildtd@si.edu FU Sichel Endowment FX This work was supported by the Sichel Endowment. NR 63 TC 5 Z9 5 U1 2 U2 14 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0011-2240 EI 1090-2392 J9 CRYOBIOLOGY JI Cryobiology PD APR PY 2014 VL 68 IS 2 BP 205 EP 214 DI 10.1016/j.cryobio1.2014.01.013 PG 10 WC Biology; Physiology SC Life Sciences & Biomedicine - Other Topics; Physiology GA AE6ZG UT WOS:000334145900007 PM 24508651 ER PT J AU Rubio, L Serra, A Chen, CYO Macia, A Romero, MP Covas, MI Sola, R Motilva, MJ AF Rubio, Laura Serra, Aida Chen, C. -Y. Oliver Macia, Alba Romero, Maria-Paz Covas, Maria-Isabel Sola, Rosa Motilva, Maria-Jose TI Effect of the co-occurring components from olive oil and thyme extracts on the antioxidant status and its bioavailability in an acute ingestion in rats SO FOOD & FUNCTION LA English DT Article ID CARDIOVASCULAR RISK-FACTORS; TANDEM MASS-SPECTROMETRY; OXIDATIVE STRESS; MEDITERRANEAN DIET; PHENOLIC-COMPOUNDS; METABOLITES; CAPACITY; BENEFITS; DISEASE; PROFILE AB The aim of this work was to examine whether bioactives in thyme could enhance the antioxidant capacity of phenolics in virgin olive oil and their bioavailability in Wistar rats. After acute oral administration of extracts from olive cake (OE), thyme (TE) or their combination (OTE), blood samples were collected from 0 to 360 min. Plasma antioxidant status was analyzed by DPPH and FRAP in plasma and by SOD, CAT and GPx activities in erythrocytes. Plasma pharmacokinetics of the main metabolites of bioactives in olive oil and thyme were characterized. Plasma non-enzymatic antioxidant capacity was significantly modulated by OE, TE, and OTE in a time-, assay, and extract-dependent manner. OE, TE, and OTE all significantly decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity and catalase (CAT) activity was increased. Pharmacokinetic results showed that plasma concentration (C-max) of the main olive phenolic metabolites in rats fed with OTE were similar to those of OE. These results indicate that an enhanced bioavailability of olive phenolic compounds could occur in the presence of thyme, although any synergistic effect was observed in the antioxidant status when both phenolic extracts were administered. Antioxidant protection by phenolics from olive and thyme against oxidative stress occurs primarily through a direct antioxidant effect and may be related to the phenolic plasmatic metabolites. C1 [Rubio, Laura; Serra, Aida; Macia, Alba; Romero, Maria-Paz; Motilva, Maria-Jose] Univ Lleida, Escola Tecn Super Engn Agr, XaRTA UTPV, Dept Food Technol, Lleida 25198, Spain. [Serra, Aida] Nanyang Technol Univ, Sch Biol Sci, Singapore 637551, Singapore. [Chen, C. -Y. Oliver] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. [Covas, Maria-Isabel] Hosp del Mar, IMIM Inst Recerca, Cardiovasc Risk & Nutr Res Grp, Barcelona 08003, Spain. [Sola, Rosa] Univ Rovira & Virgili, Fac Med & Ciencies Salut, Unitat Recerca Lipids & Arteriosclerosis, St Joan Reus Univ Hosp,CIBERDEM,IISPV, Reus 43201, Spain. RP Motilva, MJ (reprint author), Univ Lleida, Escola Tecn Super Engn Agr, XaRTA UTPV, Dept Food Technol, Avda Alcalde Rovira Roure 191, Lleida 25198, Spain. EM motilva@tecal.udl.cat RI Motilva, Maria-Jose/B-4223-2011; Macia, Alba/L-6292-2014; Rubio, Laura/L-6324-2014; SOLA, Rosa/N-5919-2014; Serra, Aida/L-6850-2016; Romero, Maria-Paz/H-3431-2011; OI Motilva, Maria-Jose/0000-0003-3823-3953; Macia, Alba/0000-0001-6091-5606; Rubio, Laura/0000-0001-8973-2942; Serra, Aida/0000-0002-4017-1096; Romero, Maria-Paz/0000-0001-9892-4874; SOLA, Rosa/0000-0002-8359-235X FU Spanish Ministry of Education and Science [AGL2012-40144-C03-01, AGL2012-40144-C03-02, AGL2012-40144-C03-03]; Spanish Ministry of Health (FIS-FEDER) [PIO21307]; CIBERDEM; CIBEROBN [CB06/03]; University of Lleida through the L. Rubio grant; DIUE de la Generalitat de Catalunya [2009 SGR 718] FX This work was supported by the Spanish Ministry of Education and Science (AGL2012-40144-C03-01, AGL2012-40144-C03-02, and AGL2012-40144-C03-03); Spanish Ministry of Health (FIS-FEDER; PIO21307), CIBERDEM, and CIBEROBN (CB06/03), and by the University of Lleida through the L. Rubio grant and DIUE de la Generalitat de Catalunya (2009 SGR 718). CIBEROBN is an initiative from Instituto de Salud Carlos III, Madrid, Spain. The authors thank Carme Pinol (in charge) from the Animal Facility Service of the University of Lleida for their technical support. NR 40 TC 4 Z9 4 U1 1 U2 14 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 2042-6496 EI 2042-650X J9 FOOD FUNCT JI Food Funct. PD APR PY 2014 VL 5 IS 4 BP 740 EP 747 DI 10.1039/c3fo60446b PG 8 WC Biochemistry & Molecular Biology; Food Science & Technology SC Biochemistry & Molecular Biology; Food Science & Technology GA AF1MF UT WOS:000334477300014 PM 24554091 ER PT J AU Sommers, WT Loehman, RA Hardy, CC AF Sommers, William T. Loehman, Rachel A. Hardy, Colin C. TI Wild land fire emissions, carbon, and climate: Science overview and knowledge needs SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Wildland fire; Climate; Forests; Carbon cycle; Emissions ID WESTERN UNITED-STATES; ALASKAN BOREAL FORESTS; LANDSCAPE DYNAMICS; CHANGE MITIGATION; PRESCRIBED FIRE; PLANT GEOGRAPHY; FUTURE; US; 21ST-CENTURY; DISTURBANCE AB Wildland fires have influenced the global carbon cycle for similar to 420 million years of Earth history, interacting with climate to define vegetation characteristics and distributions, trigger abrupt ecosystem shifts, and move carbon among terrestrial and atmospheric pools. Carbon dioxide (CO2) is the dominant driver of ongoing climate change and the principal emissions component of wildland fires, while black carbon and other aerosols found in fire emissions contribute to uncertainties in climate projections. Fire emissions research to date has been focused on developing knowledge for air pollution regulatory needs and for assessing global climate impacts. Quantifying wildland fire emissions is difficult because their amount and chemical composition vary greatly among fires depending on the amount and type of combusted fuel, its structure, arrangement, chemistry, and condition, and meteorological conditions during the fire. Prediction of potential future wildland fire emissions requires integration of complex interactions of climate, fire, and vegetation; e.g., inference about the direct effects of climate changes on vegetation (fuel) distribution, amount, and condition; direct effects on fire occurrence, behavior, and effects; and feedbacks of altered fire regimes to vegetation and the climate system. Proposed climate change mitigation strategies include management of forests for increased carbon sequestration, and because wildland fires are a key component of the carbon cycle, fire ecology, behavior, and fire effects must be accounted for in these strategies. An understanding of the complex relationships and feedbacks among climate, fire regimes, and fire emissions is needed to account for the importance of fire in the carbon cycle and wildfire and carbon feedbacks to the global climate system. Fire ecology and fire emissions science is thus a necessary component for adaptively managing landscapes and for accurately assessing the long-term effectiveness of carbon sequestration projects. This overview for a special issue on wildland fire emissions, carbon, and climate summarizes eight companion papers that describe the current state of knowledge, critical knowledge gaps, and importance of fire emissions for global climate and terrestrial carbon cycling. The goal is to foster understanding of complex fire emission system dynamics and feedbacks. (C) 2013 Elsevier B.V. All rights reserved. C1 [Sommers, William T.] George Mason Univ, Fairfax, VA 22030 USA. [Loehman, Rachel A.; Hardy, Colin C.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula Fire Sci Lab, Missoula, MT 59808 USA. RP Sommers, WT (reprint author), George Mason Univ, 1200 Univ Blvd, Fairfax, VA 22030 USA. EM wsommers@gmu.edu; raloehman@fs.fed.us; chardy01@fs.fed.us NR 129 TC 19 Z9 19 U1 7 U2 75 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 1 EP 8 DI 10.1016/j.foreco.2013.12.014 PG 8 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800001 ER PT J AU Loehman, RA Reinhardt, E Riley, KL AF Loehman, Rachel A. Reinhardt, Elizabeth Riley, Karin L. TI Wildland fire emissions, carbon, and climate: Seeing the forest and the trees - A cross-scale assessment of wildfire and carbon dynamics in fire-prone, forested ecosystems SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Carbon; Fire; Emissions; Forest ecology; Management; Resilience ID YELLOWSTONE-NATIONAL-PARK; WESTERN UNITED-STATES; ALASKAN BOREAL FORESTS; PONDEROSA PINE FORESTS; NET PRIMARY PRODUCTION; VEGETATION DISTRIBUTION; GLOBAL DISTRIBUTION; LANDSCAPE DYNAMICS; SEVERITY WILDFIRE; NORTHERN ROCKIES AB Wildfires are an important component of the terrestrial carbon cycle and one of the main pathways for movement of carbon from the land surface to the atmosphere. Fires have received much attention in recent years as potential catalysts for shifting landscapes from carbon sinks to carbon sources. Unless structural or functional ecosystem shifts occur, net carbon balance in fire-adapted systems at steady state is zero when assessed over the entire post-fire successional sequence and at landscape scales. When evaluated at fine spatial scales and over short periods of time, however, wildfires may seem to release more carbon to the atmosphere than remains on site. Measurements of wildfire carbon emissions are thus highly biased by the spatial and temporal scales that bound them, and may over-or under-estimate carbon source-sink dynamics that provide critical feedbacks to the climate system. This synthesis paper provides a description of the ecological drivers of wildfires and carbon in forested ecosystems across the spatial and temporal scales at which system drivers (e.g., climate, weather), behaviors (e.g., wildfire occurrence, spread, intensity), and resulting patterns (e.g., vegetation composition and structure, carbon emissions) occur and interact. Improved understanding of these relationships is critical if we are to anticipate and respond to major changes in the global earth system expected in the coming decades and centuries. Published by Elsevier B.V. C1 [Loehman, Rachel A.] US Forest Serv, USDA, Fire Sci Lab, Rocky Mt Res Stn, Missoula, MT 59808 USA. [Reinhardt, Elizabeth] US Forest Serv, USDA, Washington, DC 20250 USA. [Riley, Karin L.] Univ Montana, Coll Forestry & Conservat, Missoula, MT 59812 USA. RP Loehman, RA (reprint author), US Forest Serv, USDA, Fire Sci Lab, Rocky Mt Res Stn, 5775 West US Hwy 10, Missoula, MT 59808 USA. EM raloehman@fs.fed.us; ereinhardt@fs.fed.us; kriley@fs.fed.us NR 155 TC 19 Z9 19 U1 4 U2 51 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 9 EP 19 DI 10.1016/j.foreco.2013.04.014 PG 11 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800002 ER PT J AU Hao, WM Larkin, NK AF Hao, Wei Min Larkin, Narasimhan K. TI Wildland fire emissions, carbon, and climate: Wildland fire detection and burned area in the United States SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Biomass burning; Burned area; MODIS; MTBS ID TRENDS AB Biomass burning is a major source of greenhouse gases, aerosols, black carbon, and atmospheric pollutants that affects regional and global climate and air quality. The spatial and temporal extent of fires and the size of burned areas are critical parameters in the estimation of fire emissions. Tremendous efforts have been made in the past 12 years to characterize the variability of fire locations and burned areas using high frequency satellite observations (e.g., MODIS, GOES) and improved ground-based reports. We describe and compare the major global and regional (e.g., western United States) burned area products and summarize their major findings. The various ground-based reporting systems and the data quality on fire characteristics and burned areas are examined, and we summarize the major knowledge gaps and recommend further improvements in our understanding of fire activity and burned areas. Published by Elsevier B.V. C1 [Hao, Wei Min] US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. [Larkin, Narasimhan K.] US Forest Serv, Pacific Northwest Res Stn, Pacific Wildland Fire Sci Lab, Missoula, MT USA. RP Hao, WM (reprint author), US Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. EM whao@fs.fed.us NR 19 TC 3 Z9 3 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 20 EP 25 DI 10.1016/j.foreco.2013.09.029 PG 6 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800003 ER PT J AU Weise, DR Wright, CS AF Weise, David R. Wright, Clinton S. TI Wild land fire emissions, carbon and climate: Characterizing wildland fuels SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Classification; Sampling; Remote sensing; Physical; Chemical; Scaling ID CHARACTERISTIC CLASSIFICATION-SYSTEM; FOLIAR MOISTURE-CONTENT; WESTERN NORTH-AMERICA; DANGER RATING SYSTEM; UNITED-STATES; CROWN FIRE; LOBLOLLY-PINE; SURFACE FIRE; MODIS DATA; LITTER DECOMPOSITION AB Smoke from biomass fires makes up a substantial portion of global greenhouse gas, aerosol, and black carbon (GHG/A/BC) emissions. Understanding how fuel characteristics and conditions affect fire occurrence and extent, combustion dynamics, and fuel consumption is critical for making accurate, reliable estimates of emissions production at local, regional, national, and global scales. The type, amount, characteristics, and condition of wildland fuels affect combustion and emissions during wildland and prescribed fires. Description of fuel elements has focused on those needed for fire spread and fire danger prediction. Knowledge of physical and chemical properties for a limited number of plant species exists. Fuel beds with potential for high impact on smoldering emissions are not described well. An assortment of systems, methods, analytical techniques, and technologies have been used and are being developed to describe, classify, and map wildland fuels for a variety of applications. Older systems do not contain the necessary information to describe realistically the wildland fuel complex. While new tools provide needed detail, cost effectiveness to produce a reliable national fuels inventory has not been demonstrated. Climate change-related effects on vegetation growth and fuel distribution may affect the amount of GHG/A/BC emissions from wildland fires. A fundamental understanding of the relationships between fuel characteristics, fuel conditions, fire occurrence, combustion dynamics, and GHG/A/BC emissions is needed to aid strategy development to mitigate the expected effects of climate change. Published by Elsevier B.V. C1 [Weise, David R.] US Forest Serv, USDA, PSW Res Stn, Riverside, CA 92507 USA. [Wright, Clinton S.] US Forest Serv, USDA, PNW Res Stn, Seattle, WA 98103 USA. RP Weise, DR (reprint author), US Forest Serv, USDA, PSW Res Stn, 4955 Canyon Crest Dr, Riverside, CA 92507 USA. EM dweise@fs.fed.us; cwright@fs.fed.us NR 279 TC 20 Z9 20 U1 1 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 26 EP 40 DI 10.1016/j.foreco.2013.02.037 PG 15 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800004 ER PT J AU Ottmar, RD AF Ottmar, Roger D. TI Wildland fire emissions, carbon, and climate: Modeling fuel consumption SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fuel consumption; Wild land fire emissions; Carbon emissions ID BLACK CARBON; MATHEMATICAL-MODEL; COMBUSTION LIMITS; PRESCRIBED FIRES; BURNOUT MODEL; FOREST-FIRES; PEAT FIRES; MOISTURE; BIOMASS; SOILS AB Fuel consumption specifies the amount of vegetative biomass consumed during wildland fire. It is a two-stage process of pyrolysis and combustion that occurs simultaneously and at different rates depending on the characteristics and condition of the fuel, weather, topography, and in the case of prescribed fire, ignition rate and pattern. Fuel consumption is the basic process that leads to heat absorbing emissions called greenhouse gas and other aerosol emissions that can impact atmospheric and ecosystem processes, carbon stocks, and land surface reflectance. It is a critical requirement for greenhouse gas emission inventories. There are several fuel consumption models widely used by scientists and land managers including the First Order Fire Effects Model, Consume, and CanFIRE. However, these models have not been thoroughly evaluated with an independent, quality assured, fuel consumption data set. Furthermore, anecdotal evidence indicates the models have limited ability to predict consumption of specific fuel bed categories such as tree crowns, deep organic layers, and rotten logs that can contribute significantly to greenhouse gases. If we are to move forward in our ability to assess the contribution of wildland fire to greenhouse gas to the atmosphere, our current fuel consumption models must be evaluated and modified to improve their predictive capabilities. Finally, information is lacking on how much black and brown carbon from wildland fire is generated during the combustion process and how much remains on site becoming sequestered in soils, partially offsetting greenhouse gas emissions. This synthesis focuses on the process and modeling of fuel consumption and knowledge gaps that will improve our ability to predict fuel consumption and the resulting greenhouse gas emissions. Published by Elsevier B.V. C1 US Forest Serv, Pacific Northwest Res Stn, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. RP Ottmar, RD (reprint author), US Forest Serv, Pacific Northwest Res Stn, Pacific Wildland Fire Sci Lab, 400 North 34th St,Suite 201, Seattle, WA 98103 USA. EM rottmar@fs.fed.us NR 113 TC 18 Z9 19 U1 2 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 41 EP 50 DI 10.1016/j.foreco.2013.06.010 PG 10 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800005 ER PT J AU Urbanski, S AF Urbanski, Shawn TI Wildland fire emissions, carbon, and climate: Emission factors SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Biomass burning; Greenhouse gases; Emission factors ID TRANSFORM INFRARED-SPECTROSCOPY; BIOMASS-BURNING EMISSIONS; PRESCRIBED FIRES; TRACE GASES; LABORATORY MEASUREMENTS; PARTICLE EMISSIONS; UNITED-STATES; FUEL CONSUMPTION; NORTH-CAROLINA; SMOKE PLUMES AB While the vast majority of carbon emitted by wildland fires is released as CO2, CO, and CH4, wildland fire smoke is nonetheless a rich and complex mixture of gases and aerosols. Primary emissions include significant amounts of CH4 and aerosol (organic aerosol and black carbon), which are short-lived climate forcers. In addition to CO2 and short-lived climate forcers, wildland fires release CO, non-methane organic compounds (NMOC), nitrogen oxides (NOx = NO + NO2), NH3, and SO2. These species play a role in radiative forcing through their photochemical processing, which impacts atmospheric levels of CO2, CH4, tropospheric O-3, and aerosol. This paper reviews the current state of knowledge regarding the chemical composition of emissions and emission factors for fires in United States vegetation types as pertinent to radiative forcing and climate. Emission factors are critical input for the models used to estimate wildland fire greenhouse gas and aerosol emission inventories. Published by Elsevier B.V. C1 US Forest Serv, Missoula Fire Sci Lab, Rocky Mt Res Stn, Missoula, MT 59808 USA. RP Urbanski, S (reprint author), US Forest Serv, Missoula Fire Sci Lab, Rocky Mt Res Stn, 5775 US Highway 10 W, Missoula, MT 59808 USA. EM surbanski@fs.fed.us NR 58 TC 22 Z9 23 U1 9 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 51 EP 60 DI 10.1016/j.foreco.2013.05.045 PG 10 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800006 ER PT J AU Larkin, NK Raffuse, SM Strand, TM AF Larkin, Narasimhan K. Raffuse, Sean M. Strand, Tara M. TI Wildland fire emissions, carbon, and climate: US emissions inventories SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Fire emissions; Emissions inventories; Greenhouse gases ID UNITED-STATES; BIOMASS; FOREST; IMPACT; CLASSIFICATION; CHEMISTRY; AEROSOLS; FUELS; MODEL AB Emissions from wildland fire are both highly variable and highly uncertain over a wide range of temporal and spatial scales. Wildland fire emissions change considerably due to fluctuations from year to year with overall fire season severity, from season to season as different regions pass in and out of wildfire and prescribed fire periods, and from day to day as weather patterns affect large wildfire growth events and prescribed fire windows. Emissions from wildland fire are highly uncertain in that every component used to calculate wildland fire emissions is uncertain - including how much fire occurs and at what time during the year, assessments of available fuel stocks, consumption efficiency, and emissions factors used to calculate the final emissions. As shown here, these component uncertainties result in large-scale differences between estimation methods of wildland fire emissions including greenhouse gas totals, particulate matter totals, and other emissions. Four recent emissions inventories for the contiguous United States are compared to determine inter-inventory differences and to examine how methodological choices result in different annual totals and patterns of temporal and spatial variability. Inter-model variability is detailed for several current models, and current knowledge gaps and future directions for progressing fire emissions inventories are discussed. Published by Elsevier B.V. C1 [Larkin, Narasimhan K.] US Forest Serv, AirFire Team, Pacific Wildland Fire Sci Lab, Seattle, WA 98103 USA. [Raffuse, Sean M.] Sonoma Technol Inc, Petaluma, CA 94954 USA. [Strand, Tara M.] Scion Res, Christchurch 8041, New Zealand. RP Larkin, NK (reprint author), US Forest Serv, AirFire Team, Pacific Wildland Fire Sci Lab, 400 N 34th St 201, Seattle, WA 98103 USA. EM larkin@fs.fed.us FU U.S. National Fire Plan; U.S. Joint Fire Science Program [08-1-06-10]; NASA [NNX09AV76G/NNH10AN19I] FX This work was supported in part through funding from the U.S. National Fire Plan, the U.S. Joint Fire Science Program (#08-1-06-10), and NASA (#NNX09AV76G/NNH10AN19I). The authors wish to particularly thank Christine Wiedinmyer, Guido van der Werf, Jennifer Jenkins, and Ventakesh Rao for use of their emissions inventories their suggestions to improve this manuscript. The authors also wish to thank Colin Hardy, Shawn Urbanski, Roger Ottmar, and the entire USDA-Forest Service Greenhouse Gas Black Carbon Report working group for their guidance. NR 43 TC 10 Z9 10 U1 3 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 61 EP 69 DI 10.1016/j.foreco.2013.09.012 PG 9 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800007 ER PT J AU Heilman, WE Liu, YQ Urbanski, S Kovalev, V Mickler, R AF Heilman, Warren E. Liu, Yongqiang Urbanski, Shawn Kovalev, Vladimir Mickler, Robert TI Wildland fire emissions, carbon, and climate: Plume transport, and chemistry processes SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Wildland fire; Fire emissions; Plume rise; Atmospheric transport; Atmospheric chemistry ID STRATOSPHERE-TROPOSPHERE EXCHANGE; CANADIAN FOREST-FIRES; UNITED-STATES; LOWERMOST STRATOSPHERE; NORTHERN-HEMISPHERE; SMOKE PLUME; AIR-QUALITY; POLLUTION TRANSPORT; LIDAR DATA; TROPOPAUSE AB This paper provides an overview and summary of the current state of knowledge regarding critical atmospheric processes that affect the distribution and concentrations of greenhouse gases and aerosols emitted from wildland fires or produced through subsequent chemical reactions in the atmosphere. These critical atmospheric processes include the dynamics of plume rise, chemical reactions involving smoke plume constituents, the long-range transport of smoke plumes, and the potential transport of gases and aerosols from wildland fires into the stratosphere. In the area of plume-rise dynamics, synthesis information is provided on (I) the relevance of plume height for assessing impacts of gases and aerosol from wildland fires on the climate system, (2) recent scientific advances in understanding the role of multiple updraft cores in plume behavior, and (3) some of the current modeling tools and remote sensing monitoring techniques available for predicting and measuring smoke plume heights. In the area of atmospheric chemistry associated with wildland fire emissions, synthesis information is provided on what is currently known about the atmospheric fate of wildland fire smoke-plume constituents and the relationship of their atmospheric chemistry to radiative forcing. Synthesis information related to long-range atmospheric transport of wildland fire emissions is presented and summarizes many of the recent published observational and modeling studies that provide clear evidence of intercontinental, continental, and regional transport of North American fire emissions, including black carbon, to locations far-removed from the fire-event locations. Recent studies are also highlighted that examined the significance of troposphere-stratosphere exchange processes, which can result in the transport of greenhouse gases and aerosols from North American wildland fires into the stratosphere where they can remain for very long periods of time and alter the radiative balance and typical chemical reactions that occur there. Finally, specific research gaps and needs related to plume dynamics, atmospheric transport and deposition processes, and the atmospheric chemistry of wildland fire emissions are identified and discussed. Published by Elsevier B.V. C1 [Heilman, Warren E.] USDA, Forest Serv, No Res Stn, E Lansing, MI 48823 USA. [Liu, Yongqiang] USDA, Forest Serv, Ctr Forest Disturbance Sci, Athens, GA 30602 USA. [Urbanski, Shawn; Kovalev, Vladimir] USDA, Forest Serv, Rocky Mt Res Stn, Missoula, MT 59808 USA. [Mickler, Robert] Alion Sci & Technol Corp, Durham, NC 27713 USA. RP Heilman, WE (reprint author), USDA, Forest Serv, No Res Stn, 1407 S Harrison Rd, E Lansing, MI 48823 USA. EM wheilman@fs.fed.us; yliu@fs.fed.us; surbanski@fs.fed.us; vkovalev@fs.fed.us; rmickler@alionscience.com FU USDA Forest Service's Northern Research Station; Southern Research Station; Rocky Mountain Research Station FX We would like to thank the USDA Forest Service's Northern Research Station, Southern Research Station, and Rocky Mountain Research Station for the support received for this synthesis study. The editorial comments and technical reviews provided by William Sommers, Susan Conard, Stanley Coloff, and the anonymous Forest Ecology and Management reviewers are appreciated. NR 110 TC 15 Z9 15 U1 4 U2 53 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 70 EP 79 DI 10.1016/j.foreco.2013.02.001 PG 10 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800008 ER PT J AU Liu, YQ Goodrick, S Heilman, W AF Liu, Yongqiang Goodrick, Scott Heilman, Warren TI Wildland fire emissions, carbon, and climate: Wildfire-climate interactions SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Wildfire; Emission; Radiative forcing; Feedback to climate; Future fire projection ID BIOMASS BURNING EMISSIONS; NINO-SOUTHERN-OSCILLATION; AEROSOL OPTICAL DEPTH; WESTERN UNITED-STATES; BLACK CARBON; FOREST-FIRES; INTERANNUAL VARIABILITY; CHANGING CLIMATE; EL-NINO; VEGETATION DISTRIBUTION AB Increasing wildfire activity in recent decades, partially related to extended droughts, along with concern over potential impacts of future climate change on fire activity has resulted in increased attention on fire-climate interactions. Findings from studies published in recent years have remarkably increased our understanding of fire-climate interactions and improved our capacity to delineate probable future climate change and impacts. Fires are projected to increase in many regions of the globe under a changing climate due to the greenhouse effect. Burned areas in the western US could increase by more than 50% by the middle of this century. Increased fire activity is not simply an outcome of the changing climate, but also a participant in the change. Smoke particles reduce overall solar radiation absorbed by the Earth's atmosphere during individual fire events and fire seasons, leading to regional climate effects including reduction in surface temperature, suppression of cloud and precipitation, and enhancement of climate anomalies such as droughts. Black carbon (BC) in smoke particles displays some different radiation and climate effects by warming the middle and lower atmosphere, leading to a more stable atmosphere. BC also plays a key role in the smoke-snow feedback mechanism. Fire emissions of CO2, on the other hand, are an important atmospheric CO2 source and contribute substantially to the global greenhouse effect. Future studies should generate a global picture of all aspects of radiative forcing by smoke particles. Better knowledge is needed in space and time variability of smoke particles, evolution of smoke optical properties, estimation of smoke plume height and vertical profiles and their impacts on locations of warming layers, stability structure, clouds and smoke transport, quantification of BC emission factors and optical properties from different forest fuels, and BC's individual and combined roles with organic carbon. Finally, understanding the short-and long-term greenhouse effect of fire CO2 emissions, increased capacity to project future fire trends (especially mega-fires), with consideration of climate-fuel-human interactions, and improved fire weather and climate prediction skills (including exploring the SST-fire relations) remain central knowledge needs. Published by Elsevier B.V. C1 [Liu, Yongqiang; Goodrick, Scott] USDA, Forest Serv, Southern Res Stn, Ctr Forest Disturbance Sci, Athens, GA 30602 USA. [Heilman, Warren] USDA, Forest Serv, No Res Stn, E Lansing, MI 48823 USA. RP Liu, YQ (reprint author), USDA, Forest Serv, Southern Res Stn, Ctr Forest Disturbance Sci, 320 Green St, Athens, GA 30602 USA. EM yliu@fs.fed.us; wheilman@fs.fed.us FU FS Southern Research Station; Northern Research Station; USDA/USDI [JFSP 11172]; USDA NIFA [2013-35100-20516] FX This study was part of a synthesis project on greenhouse gases and aerosols from wildland emissions, within the USDA Forest Service Research and Development. The authors would like to thank the FS Southern Research Station and the Northern Research Station for their support, the insightful and constructive comments and suggestions from two anonymous reviewers, and edits provided by Bill Sommers, Sue Conard, and Stanley Coloff of George Mason University and by Lane Eskew of U.S. Forest Service Rocky Mountain Research Station. The study was partially supported by the USDA/USDI Joint Fire Science Program under Agreement No. JFSP 11172, and the USDA NIFA Project 2013-35100-20516. NR 182 TC 27 Z9 27 U1 12 U2 94 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD APR 1 PY 2014 VL 317 SI SI BP 80 EP 96 DI 10.1016/j.foreco.2013.02.020 PG 17 WC Forestry SC Forestry GA AE6UZ UT WOS:000334134800009 ER PT J AU MacLean, RG Ducey, MJ Hoover, CM AF MacLean, Richard G. Ducey, Mark J. Hoover, Coeli M. TI A Comparison of Carbon Stock Estimates and Projections for the Northeastern United States SO FOREST SCIENCE LA English DT Article DE biomass; growth and yield; model validation; carbon markets ID FOREST VEGETATION SIMULATOR; BIOMASS EXPANSION FACTORS; MODEL VALIDATION; EQUATIONS; TREES; TESTS; FVS AB We conducted a comparison of carbon stock estimates produced by three different methods using regional data from the USDA Forest Service Forest Inventory and Analysis (FIA). Two methods incorporated by the Forest Vegetation Simulator (FIN were compared to each other and to the current HA component ratio method. We also examined the uncalibrated performance of FVS growth simulations for predicting net carbon accumulation in live trees. In general, the three carbon stock estimation approaches do not produce estimates that are either equivalent or are simply convertible. A strong spatial pattern of relationships between estimates was associated with regional variation in stand top height. Uncalibrated growth projections gave downwardly biased results that were also poorly correlated with observed carbon accumulation rates, yielding little improvement in root mean square error over the use of a simple regional average. These results reinforce the need for managers and scientists to be careful in choosing methods and reporting carbon stock estimates and to use appropriate model calibration methods in projecting future carbon accumulation. C1 [MacLean, Richard G.] Univ New Hampshire, Durham, NH 03824 USA. [Ducey, Mark J.] Univ New Hampshire, Dept Nat Resources, Durham, NH 03824 USA. [Hoover, Coeli M.] USDA, Forest Service, Washington, DC USA. RP MacLean, RG (reprint author), Univ New Hampshire, Durham, NH 03824 USA. EM rich.maclean@unh.edu; mjducey@unh.edu; choover@fs.fed.us RI Ducey, Mark/K-1101-2016 FU USDA Forest Service [RJVA 09-JV-11242302110]; University of New Hampshire [RJVA 09-JV-11242302110] FX This project was supported by Research Joint Venture Agreement RJVA 09-JV-11242302110 between the USDA Forest Service and the University of New Hampshire. We gratefully acknowledge the constructive comments of three reviewers and the associate editor. NR 39 TC 8 Z9 8 U1 1 U2 23 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 206 EP 213 DI 10.5849/forsci.12-072 PG 8 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400002 ER PT J AU Greenberg, CH Keyser, CE Rathbun, LC Rose, AK Fearer, TM McNab, WH AF Greenberg, Cathryn H. Keyser, Chad E. Rathbun, Leah C. Rose, Anita K. Fearer, Todd M. McNab, W. Henry TI Forecasting Long-Term Acorn Production with and without Oak Decline Using Forest Inventory Data SO FOREST SCIENCE LA English DT Article DE acorn; Forest Vegetation Simulator; hard mast; oak decline; upland hardwood forest ID EASTERN UNITED-STATES; PREDICTION MODELS; HARD-MAST; MOUNTAINS; PATTERNS AB Acorns are important as wildlife food and for oak regeneration, but production is highly variable, posing a challenge to forest managers targeting acorn production levels. Forest managers need tools to predict acorn production capability tailored to individual landscapes and forest management scenarios, adjusting for oak mortality and stand development over time. We implemented published predictive models of average annual acorn production by five oak species common to the eastern United States in the Forest Vegetation Simulator (FVS) and used forest inventory data to estimate long-term acorn production on the Bent Creek Experimental Forest watershed, with and without oak decline. Under a no-management scenario, simulations forecasted a 58% increase in average annual acorn production by 2062 without oak decline but a 17% decrease with oak decline. Forecasts were influenced by the initial abundance and basal area of different oak species on the landscape and stand dynamics over time. Simulations indicated that heavy oak mortality with regeneration failure could substantially affect acorn production over the long term by reducing the proportion of mature canopy oaks and relative abundance of oak species. FVS ACORN provides a powerful tool for long-term acorn production planning that can be tailored to individual landscapes and forest management scenarios to predict average annual number and mass of acorns. C1 [Greenberg, Cathryn H.] USDA, Forest Serv, Southern Res Stn, Bent Creek Expt Forest, Asheville, NC 28804 USA. [Keyser, Chad E.; Rathbun, Leah C.] USDA, Forest Serv, Forest Management Serv Ctr, Asheville, NC USA. [Rose, Anita K.; McNab, W. Henry] USDA, Forest Serv, Southern Res Stn, Asheville, NC USA. RP Greenberg, CH (reprint author), USDA, Forest Serv, Southern Res Stn, Bent Creek Expt Forest, Asheville, NC 28804 USA. EM kgreenberg@fs.fed.us; ckeyser@fs.fed.us; lrathbun@fs.fed.us; anitarose@fs.fed.us; tfearer@abcbirds.org; hmcnab@fs.fed.us NR 37 TC 1 Z9 1 U1 2 U2 12 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 222 EP 230 DI 10.5849/forsci.12-106 PG 9 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400004 ER PT J AU Noonan-Wright, EK Valliant, NM Reiner, AL AF Noonan-Wright, Erin K. Valliant, Nicole M. Reiner, Alicia L. TI The Effectiveness and Limitations of Fuel Modeling Using the Fire and Fuels Extension to the Forest Vegetation Simulator SO FOREST SCIENCE LA English DT Article DE prescribed fire; custom fuel model; California; fire behavior modeling ID CONIFEROUS FORESTS; UNITED-STATES; CROWN FIRE; BEHAVIOR; CALIFORNIA; SPREAD AB Fuel treatment effectiveness is often evaluated with fire behavior modeling systems that use fuel models to generate fire behavior outputs. How surface fuels are assigned, either using one of the 53 stylized fuel models or developing custom fuel models, can affect predicted fire behavior. We collected surface and canopy fuels data before and 1, 2, 5, and 8 years after prescribed fire treatments across 10 national forests in California. Two new methods of assigning fuel models within the Fire and Fuels Extension to the Forest Vegetation Simulator were evaluated. Field-based values for dead and downed fuel loading were used to create custom fuel models or to assign stylized fuel models. Fire was simulated with two wind scenarios (maximum 1-minute speed and maximum momentary gust speed) to assess the effect of the fuel model method on potential fire behavior. Surface flame lengths and fire type produced from custom fuel models followed the fluctuations and variability of fine fuel loading more closely than stylized fuel models. However, results of 7 out of 10 statistical tests comparing surface flame length between custom and stylized fuel models were not significant (P < 0.05), suggesting that both methods used to assign surface fuel loads will predict fairly similar trends in fire behavior. C1 [Noonan-Wright, Erin K.] USDA, Forest Serv, Wildand Fire Management Res Dev & Applicat, Missoula, MT USA. [Valliant, Nicole M.] USDA, Forest Serv, Serv Western Wildland Environm Threat Assessment, Missoula, MT USA. [Reiner, Alicia L.] USDA, Forest Serv, Adapt Management Serv Enterprise Team, Missoula, MT USA. RP Noonan-Wright, EK (reprint author), USDA, Forest Serv, Wildand Fire Management Res Dev & Applicat, Missoula, MT USA. EM enoonan02@fs.fed.us; nvaillant@fs.fed.us; alreiner@fs.fed.us FU Joint Fire Science Grant [09-1-01-1] FX We thank Dr. JoAnn Fites-Kaufman for initiating this monitoring project. We thank Scott Dailey, Carol Ewell, Kevin McCrummen, Todd Decker, and all the field crew members over the past 12 years. Finally, we thank Lane Eskew for his technical review of this manuscript. Funding for this project was provided by a Joint Fire Science Grant (09-1-01-1). NR 55 TC 3 Z9 3 U1 4 U2 9 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 231 EP 240 DI 10.5849/forsci.12-062 PG 10 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400005 ER PT J AU Hudak, AT Haren, AT Crookston, NL Liebermann, RJ Ohmann, JL AF Hudak, Andrew T. Haren, A. Tod Crookston, Nicholas L. Liebermann, Robert J. Ohmann, Janet L. TI Imputing Forest Structure Attributes from Stand Inventory and Remotely Sensed Data in Western Oregon, USA SO FOREST SCIENCE LA English DT Article DE gradient nearest neighbor (GNN); Landsat; LandTrendr; LiDAR; most similar neighbor (MSN); k nearest neighbor (k-NN); random forest (RF) ID NEAREST-NEIGHBOR IMPUTATION; SATELLITE IMAGERY; NATIONAL FOREST; LIDAR; MANAGEMENT; VEGETATION; DATABASES; ACCURACY; DENSITY; PLOTS AB Imputation is commonly used to assign reference stand observations to target stands based on covariate relationships to remotely sensed data to assign inventory attributes across the entire landscape. However, most remotely sensed data are collected at higher resolution than the stand inventory data often used by operational foresters. Our primary goal was to compare various aggregation strategies for modeling and mapping forest attributes summarized from stand inventory data, using predictor variables derived from either light detection and ranging (LiDAR) or Landsat and a US Geological Survey (USGS) digital terrain model (DIM). We found that LiDAR metrics produced more accurate models than models using Landsat/USGS-DTM predictors. Calculating stand-level means of all predictors or all responses proved most accurate for developing imputation models or validating imputed maps, respectively. Developing models or validating maps at the unaggregated scale of individual stand subplots proved to be very inaccurate, presumably due to poor geolocation accuracies. However, using a sample of pixels within stands proved only slightly less accurate than using all available pixels. Furthermore, bootstrap tests of similarity between imputations and observations showed no evidence of bias regardless of aggregation strategy. We conclude that pixels sampled from within stands provide sufficient information for modeling or validating stand attributes of interest to foresters. C1 [Hudak, Andrew T.; Crookston, Nicholas L.; Liebermann, Robert J.] USDA, Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. [Haren, A. Tod] Oregon Dept Forestry, Salem, OR USA. [Ohmann, Janet L.] Pacific NW Res Stn, USDA, Forest Serv, Corvallis, OR USA. RP Hudak, AT (reprint author), USDA, Forest Serv, Rocky Mt Res Stn, Moscow, ID USA. EM ahudak@fs.fed.us; tharen@odf.state.or.us; ncrookston@fs.fed.us; rjliebermann@fs.fed.us; janet.ohmann@orst.edu FU Oregon Department of Forestry [11-CO-11221633-136] FX We thank Dave Enck for assistance in interpreting the SLI protocol, Emmor Nile for obtaining 2007-2009 LiDAR data, Jim Muckenhoupt for providing 2010 LiDAR data, Justin Braaten and Robert Kennedy for providing LandTrendr data, and Jeff Hamann for productive conversations about this project. Funding for this research was provided by the Oregon Department of Forestry through Collective Agreement 11-CO-11221633-136. NR 61 TC 7 Z9 7 U1 0 U2 19 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 253 EP 269 DI 10.5849/forsci.12-101 PG 17 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400007 ER PT J AU Crotteau, JS Ritchie, MW Varner, JM AF Crotteau, Justin S. Ritchie, Martin W. Varner, J. Morgan TI A Mixed-Effects Heterogeneous Negative Binomial Model for Postfire Conifer Regeneration in Northeastern California, USA SO FOREST SCIENCE LA English DT Article DE burn severity; natural regeneration; ponderosa pine; white fir; zero-inflated negative binomial; Storrie Fire; stocking analysis ID INFLATED POISSON REGRESSION; FIRE SEVERITY; SIERRA-NEVADA; SOUTHERN CASCADES; TREE REGENERATION; PINUS-PONDEROSA; MIXTURE-MODELS; BURN SEVERITY; COUNT DATA; FOREST AB Many western USA fire regimes are typified by mixed-severity fire, which compounds the variability inherent to natural regeneration densities in associated forests. Tree regeneration data are often discrete and nonnegative; accordingly, we fit a series of Poisson and negative binomial variation models to conifer seedling counts across four distinct burn severities and three forest types 10 years after the 23,000-ha Storrie Fire, a large mixed-severity fire in northern California. Despite the accessibility and power of the zero-inflated negative binomial mixture model, a flexible heterogeneous negative binomial model offered a superior fit. Incorporation of a random stand effect further improved model performance. A parametric bootstrap analysis was conducted to examine seedling distributions and stand stocking. Mean simulated seedling densities had an expansive range (272-29,257 ha(-1)). Stocking analyses suggest a high probability of deficient conifer coverage in the majority of lower-elevation high-severity burn stands. In addition, models were fit to fir and pine seedling counts. Only a minority of postfire stands were likely to be stocked in the pine-only analysis. These models will help land managers prioritize limited resources for artificial reforestation in mixed-severity burned landscapes. Keywords: burn severity, natural regeneration, ponderosa pine, white fir, zero-inflated negative binomial, Storrie Fire, stocking analysis C1 [Crotteau, Justin S.] Humboldt State Univ, Redding, CA USA. [Crotteau, Justin S.] PSW Res Stn, USDA, Forest Serv, PSW Redding, Redding, CA USA. [Ritchie, Martin W.] PSW Res Stn, USDA, Forest Serv, Washington, DC USA. [Varner, J. Morgan] Mississippi State Univ, Mississippi State, MS USA. RP Crotteau, JS (reprint author), Humboldt State Univ, Redding, CA USA. EM justin.crotteau@gmail.com; mritchie@fs.fed.us; mvarner@cfr.msstate.edu FU Lassen National Forest, Almanor Ranger District FX Support for this article was provided by the Lassen National Forest, Almanor Ranger District. Data collection was performed by US Department of Agriculture PSW Redding's Vegetation Dynamics team. Jim Baldwin's statistical insight and guidance was integral to our model development and interpretation. NR 63 TC 6 Z9 6 U1 4 U2 20 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 275 EP 287 DI 10.5849/forsci.12-089 PG 13 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400009 ER PT J AU Song, NF Aguilar, FX Butler, BJ AF Song, Nianfu Aguilar, Francisco X. Butler, Brett J. TI Conservation Easements and Management by Family Forest Owners: A Propensiiy Score Matching Approach with Multi-Imputations of Survey Data SO FOREST SCIENCE LA English DT Article DE selection bias; conservation easement; propensity score matching; multiple imputation; missing data ID ECONOMETRIC EVALUATION ESTIMATOR; SELECTION BIAS; PRESERVATION PROGRAMS; TRAINING-PROGRAMS; CAUSAL; IMPLEMENTATION; BIODIVERSITY AB Increasingly, private landowners are participating in conservation easement programs, but their effects on land management remain to be addressed. Data from the USDA Forest Service National Woodland Owner Survey for the US Northern Region were used to investigate how conservation easement participation is associated with selected past and future forest management practices. Multiple data imputation was used to correct for missing data bias, and propensity score matching was applied to correct for selection bias. Results show that only the adoption of forest management plans, among 17 forest management practices, was significantly and positively correlated with easement participation. Conservation easements legally bind participants to maintain land forested, but there was no evidence of greater association between easement participation and active forest management practices, including timber harvesting. These findings suggest that adoption of conservation easements is a policy tool that can preserve forestland from changing to other uses but may not necessarily be conducive to wider implementation of land practices necessary for long-term protection of forests. C1 [Song, Nianfu] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Aguilar, Francisco X.] Univ Missouri, Columbia, MO 65211 USA. [Butler, Brett J.] USDA, Forest Serv, Washington, DC USA. RP Song, NF (reprint author), Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. EM songn@missouri.edu; aguilarf@missouri.edu; bbutler01@fs.fed.us FU USDA Forest Service [09-JV-11242311-008] FX This research was supported in part by the USDA Forest Service through agreement number 09-JV-11242311-008. NR 54 TC 5 Z9 5 U1 6 U2 27 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 298 EP 307 DI 10.5849/forsci.12-107 PG 10 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400011 ER PT J AU Reich, RM Lundquist, JE Acciavatti, RE AF Reich, Robin M. Lundquist, John E. Acciavatti, Robert E. TI Influence of Climatic Conditions and Elevation on the Spatial Distribution and Abundance of Trypodendron ambrosia Beetles (Coleoptera: Curculionidae: Scolytinae) in Alaska SO FOREST SCIENCE LA English DT Article DE aerial survey; negative binomial regression; pest survey; spatial pest models ID INSECTS AB The objective of this study was to model the influence of temperature and precipitation on the distribution and abundance of the ambrosia beetles in the genus Trypodendron. Although these beetles do not attack and kill healthy trees, their gallery holes and accompanying black and gray stain associated with symbiotic ambrosial fungi can cause significant economic losses to commercial logs and wood products. Beetles were collected along a 1,100-km latitudinal transect 4 times at 2-week intervals at 43 sites beginning early July using Lindgren-funnel traps baited with ethanol, alpha-pinene, and lineatin. Average annual temperature and precipitation were used to partition the state into 25 climatic zones. Large-scale patterns of beetle distribution were correlated with elevation and the temperature and precipitation zones. Results indicate that reasonably accurate predictions of beetle abundance can be generated using models based on trap data collected across several climate zones. Predictions derived from this latitudinal transect can be extrapolated to more remote areas using species environment relationships based on temperature and precipitation combinations. Partitioning large geographic areas using climatic zones offers a logical approach for predicting insect activity in remote areas, and if implemented on a long-term basis would be able to provide estimates of yearly and seasonal trends in the infestation. Such information would allow forest managers to evaluate the impact on forest ecosystem services and improve future assessments that predict the influence of a changing climate on insect pest migrations and their intensification during outbreaks. C1 [Reich, Robin M.] Colorado State Univ, Ft Collins, CO 80523 USA. [Lundquist, John E.] USDA, Forest Serv, Anchorage, AK USA. [Acciavatti, Robert E.] Carnegie Museum Nat History, Pittsburgh, PA 15213 USA. RP Reich, RM (reprint author), Colorado State Univ, Ft Collins, CO 80523 USA. EM Robin@cnr.colostate.edu; jlundquist@fs.fed.us; auriebob@mail.wvnet.edu FU Western Wildland Environmental Threat Assessment Center FX We wish to thank Rick Kelsey, Yu Wei, and Ashley Steele for reviewing and editing earlier versions of this manuscript. This study was funded in part by the Western Wildland Environmental Threat Assessment Center. NR 35 TC 4 Z9 4 U1 4 U2 27 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 308 EP 316 DI 10.5849/forsci.12-092 PG 9 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400012 ER PT J AU Gray, AN Whittier, TR Azuma, DL AF Gray, Andrew N. Whittier, Thomas R. Azuma, David L. TI Estimation of Aboveground Forest Carbon Flux in Oregon: Adding Components of Change to Stock-Difference Assessments SO FOREST SCIENCE LA English DT Article DE carbon accounting; land-use change; disturbance; management; forest inventory ID NET ECOSYSTEM PRODUCTION; UNITED-STATES; DETECTING TRENDS; MODELS; SEQUESTRATION; DISTURBANCE; PRODUCTS; HISTORY; BALANCE AB A substantial portion of the carbon (C) emitted by human activity is apparently being stored in forest ecosystems in the Northern Hemisphere, but the magnitude and cause are not precisely understood. Current official estimates of forest C flux are based on a combination of field measurements and other methods. The goal of this study was to improve on existing methods by directly tracking components of change in tree C across a large region using field measurements. We used repeated Forest Inventory and Analysis (FIA) measurements on permanent plots to quantify aboveground live tree C flux over an 11-year period due to land-use change, disturbance, and harvest, on 1,073 plots across 5.96 million ha of nonfederal forestland in Oregon. Land-use change resulted in a 110,000 ha (1.9%) net increase of forestland between 1986 and 1997. However, there was a net loss of 3.4 Tg of live tree C due to land-use change because the forestland lost was more productive than that gained. Live woody C decreased significantly in eastern Oregon (-14.4 Tg), with mortality and harvest exceeding growth, primarily due to severe defoliation by western spruce budworm. However, C stores increased significantly in western Oregon (19.2 Tg) due primarily to large accumulations from growth on nonfederal public lands. We demonstrate that C accounting that uses remeasured probabilistic field sample data can produce detailed estimates of C flux that identify causes and components of change and produce more consistent estimates than alternative approaches. C1 [Gray, Andrew N.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. [Whittier, Thomas R.] Oregon State Univ, Forest Ecosyst & Soc Dept, Corvallis, OR 97331 USA. [Azuma, David L.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR USA. RP Gray, AN (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, Corvallis Forestry Sci Lab, Corvallis, OR 97331 USA. EM agray01@fs.fed.us FU USDA Forest Service Pacific Northwest Research Station; Oregon State University College of Forestry FX Don Gedney and Neil McKay helped design the periodic inventories in Oregon and numerous dedicated crews collected the field and photo-interpreted data. This study was funded by the USDA Forest Service Pacific Northwest Research Station with support from Oregon State University College of Forestry. The careful comments of two anonymous reviewers improved the manuscript. NR 57 TC 4 Z9 4 U1 1 U2 17 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 317 EP 326 DI 10.5849/forsci.12-139 PG 10 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400013 ER PT J AU Gustafson, EJ AF Gustafson, Eric J. TI Applicability of Predictive Models of Drought-Induced Tree Mortalily between the Midwest and Northeast United States SO FOREST SCIENCE LA English DT Article DE drought stress; climate change; forest biomass; Northeastern Mixed Forest Province; Laurentian mixed forest province ID CLIMATE-CHANGE; FOREST; DISTURBANCE; LANDSCAPE; AMERICA; DECLINE; GROWTH AB Regression models developed in the upper Midwest (United States) to predict drought-induced tree mortality from measures of drought (Palmer Drought Severity Index) were tested in the northeastern United States and found inadequate. The most likely cause of this result is that long drought events were rare in the Northeast during the period when inventory data were available. Therefore, new predictive models of drought mortality for the Northeast were built using USDA Forest Service inventory data and national climate data from 1969 to 2007. The Standardized Precipitation Index was better correlated with tree mortality in the Northeast than the Palmer Drought Severity Index, and new models were estimated. The reliability of the northeast models varied considerably by drought-sensitivity class, with the model for drought-intolerant species being particularly suspect. I argue that the Midwest models may nevertheless have some value in the Northeast because my tests were unable to cover the range of drought conditions under which the models were developed, there is no obvious reason why the same species should respond differently in a very similar ecological province, and some northeast models are very weak. C1 US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI 54501 USA. RP Gustafson, EJ (reprint author), US Forest Serv, USDA, No Res Stn, Inst Appl Ecosyst Studies, Rhinelander, WI 54501 USA. EM egustafson@fs.fed.us FU USDA Forest Service Northern Research Station FX I thank John Brissette, Dave Cleland, and Brian Sturtevant for enlightening discussions of ecological response to drought, Scott Pugh for advice on manipulating the FIA database, Laura Kenefic, Joshua Puhlick, and Justin Waskiewicz for assistance with the Penobscot Experimental Forest data and Inge Seaboyer, Bruce Allen, and Ken Desmarais for assistance in my attempt to use the Fox Research Forest (New Hampshire) data, and Tina Baumann for GIS assistance. Elaine Qualtiere, John Brissette, Jane Foster, and two anonymous reviewers provided helpful reviews that greatly improved the manuscript. Funding was provided by the USDA Forest Service Northern Research Station. NR 31 TC 2 Z9 2 U1 1 U2 13 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 327 EP 334 DI 10.5849/forsci.12-148 PG 8 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400014 ER PT J AU Kern, CC Montgomery, RA Reich, PB Strong, TF AF Kern, Christel C. Montgomery, Rebecca A. Reich, Peter B. Strong, Terry F. TI Harvest-Created Canopy Gaps Increase Species and Functional Trait Diversity of the Forest Ground-Layer Communiiy SO FOREST SCIENCE LA English DT Article DE understory plants; species richness; gap size; forest scenario simulation; natural disturbance-based silviculture; additive partitioning of richness; resampling; estimation ID NORTHEASTERN NORTH-AMERICA; NATURAL DISTURBANCE REGIMES; HEMLOCK-HARDWOOD FORESTS; PLANT-COMMUNITIES; GROUP SELECTION; HERB LAYER; UNDERSTOREY VEGETATION; SILVICULTURAL SYSTEMS; ECOSYSTEM PROCESSES; DECIDUOUS FORESTS AB Biodiversity conservation within managed forests depends, in part, on management practices that restore or maintain plant community diversity and function. Because many plant communities are adapted to natural disturbances, gap-based management has potential to meet this need by using the historical range of variation in canopy disturbances to guide elements of harvest design. We tested this hypothesis with a well-replicated gap size experiment in a second-growth northern hardwood forest. We evaluated plant communities within and among experimental gaps of differing size, 13 years after an initial harvest. We used a resampling approach to estimate how conventional and gap-based management affect diversity partitioning and species and trait diversity of ground-layer plants. These diversity measures highlight relevant scales and function of ground-layer plants among harvest gap sizes and scenarios. Results from our field experiment showed that, at the gap-level, increasing gap size increased functional trait diversity of plants, while species diversity was higher in gaps than uncut references and maximized in medium-sized (20-30 m diameter) gaps. In harvest scenarios created by resampling our empirical data, we found that at the stand scale, increasing harvest intensity (larger gaps and greater proportion of forest in gaps) increased species richness and variability in initial bloom times, tolerance to shade, and number of life-forms in the plant community. Based on the measures of diversity and function used in our study, our results suggest that size of harvest-created gaps and proportion of forest in gaps can be manipulated to attain biodiversity goals but evaluating the regional species pool and seed sources (e.g., presence of invasives, rare species) will be important to maintain or restore conservation value. C1 [Kern, Christel C.; Strong, Terry F.] US Forest Serv, USDA, Grand Rapids, MN 55744 USA. [Montgomery, Rebecca A.; Reich, Peter B.] Univ Minnesota, Minneapolis, MN 55455 USA. RP Kern, CC (reprint author), US Forest Serv, USDA, Grand Rapids, MN 55744 USA. EM cckern@fs.fed.us; rebeccam@umn.edu; preich@umn.edu RI Montgomery, Rebecca/J-5774-2013 OI Montgomery, Rebecca/0000-0002-4131-1847 FU USDA Forest Service FX This study was supported by the USDA Forest Service. We thank two anonymous reviewers, Brian Palik, Anthony D'Amato, John Zasada, Adam Weise, Kris Gray, and Emma Schultz. NR 77 TC 10 Z9 11 U1 2 U2 55 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 335 EP 344 DI 10.5849/forsci.13-015 PG 10 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400015 ER PT J AU Peterson, RL Liang, JJ Barrett, TM AF Peterson, Randy L. Liang, Jingjing Barrett, Tara M. TI Modeling Population Dynamics and Woody Biomass in Alaska Coastal Forest SO FOREST SCIENCE LA English DT Article DE matrix model; forest management; population structure; spatial autocorrelation ID SPATIAL DEPENDENCE; GROWTH; MANAGEMENT; DIVERSITY; SPRUCE; VALUES; INDEX AB Alaska coastal forest, 6.2 million ha in size, has been managed in the past mainly through clearcutting. Declining harvest and dwindling commercial forest resources over the past 2 decades have led to increased interest in management of young-growth stands and utilization of woody biomass for bioenergy. However, existing models to support these new management systems are very limited in number and value. This study presents a density-dependent, size-specific, and species-specific matrix growth model for forest in coastal Alaska. This model enables short- and long-term predictions of stand basal area, volume, and biomass in a simple and accurate way and facilitates understanding of the ecological and economic effects of forest management alternatives. Components of forest growth were estimated from tree- and stand-level attributes from repeated measurements of 544 Forest Inventory and Analysis permanent sample plots located throughout coastal Alaska with a wide range of stand conditions. The model was tested on 293 postsample validation plots and found to have significantly higher accuracy than the Forest Vegetation Simulator, the only growth model available for the region. Analysis of residuals revealed no spatial autocorrelation, which indicates that this model is able to adequately account for the effects of physiographic factors and other differences between southeast and southcentral Alaska. C1 [Peterson, Randy L.; Liang, Jingjing] Univ Alaska Fairbanks, Fairbanks, AK 99775 USA. [Barrett, Tara M.] US Forest Serv, USDA, Pacific NW Res Stn, Washington, DC USA. RP Peterson, RL (reprint author), Univ Alaska Fairbanks, Fairbanks, AK 99775 USA. EM alpenbering@gmail.com NR 46 TC 2 Z9 3 U1 4 U2 15 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 391 EP 401 DI 10.5849/forsci.12-540 PG 11 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400021 ER PT J AU Bartos, DL Tshireletso, K Malechek, JC AF Bartos, Dale L. Tshireletso, Koketso Malechek, John C. TI Response of Aspen Suckers to Simulated Browsing SO FOREST SCIENCE LA English DT Article DE quaking aspen; browsing intensity; browsing season; herbivory; Populus tremuloides ID POPULUS-TREMULOIDES; QUAKING ASPEN; TREMBLING ASPEN; PLANT-GROWTH; HERBIVORY; REGENERATION; DEFOLIATION; TOLERANCE; BIOMASS; CATTLE AB Heavy and repeated ungulate browsing on reproductive suckers has limited quaking aspen (Populus tremuloides Michx.) regeneration on many Western landscapes. However, little is known about the specific effects of season and intensity of browsing. The objectives of this study were to determine the effects of season and intensity of clipping (simulated browsing) on density and growth characteristics of suckers. Three randomly selected stands were clear-felled in mid-July 2005 and fenced. Simulated browsing intensities of 0, 20, 40, and 60% removal of the current year's growth on aspen suckers were randomly applied in early, mid, and late summers of 2006 and 2007 on permanently demarcated quadrats. Sucker density and sucker height were monitored in each quadrat. Changes in annual sucker numbers for the ends of 2006 and 2007 were compared with declines found that reflected both winter and summer (2007 only) mortality. Mortality was not found for suckers clipped in early summer compared with mortalities of 48 and 46% for mid and late summer clipped suckers, respectively. However, even at the highest mortality, there were still ample numbers (similar to 25,000/ha) of suckers for stand regeneration. At the end of the study, sucker height was reduced by all summer treatment intensities. After adjustment for the controls, clipping (of current year's growth) at 20 and 40% in mid and late summer resulted in suckers that were 47 and 41 cm tall, respectively. However, clipping at 60% resulted in suckers that were only 20 cm tall. Application of information found in this study to new areas under similar circumstances suggests that browsing of terminal leaders at intensities <= 40% in midsummer for 1 or 2 years would not have a substantial impact on aspen regeneration. C1 [Bartos, Dale L.] US Forest Serv, USDA, Forestry Sci Lab, Logan, UT USA. [Tshireletso, Koketso] Botswana Coll Agr, Gaborone, Botswana. [Malechek, John C.] Utah State Univ, Wildand Resources Dept, Logan, UT 84322 USA. RP Bartos, DL (reprint author), US Forest Serv, USDA, Forestry Sci Lab, Logan, UT USA. EM dalelbartos@fs.fed.us; ktshirel@bca.bw; john.malechek@aggiemail.usu.edu FU Utah Agricultural Experiment Station through the Cedar Mountain Initiative FX The research was funded by the Utah Agricultural Experiment Station through the Cedar Mountain Initiative. We wish to thank particularly the Utah Agriculture Experiment Station staff located in Cedar City, UT who provided valuable support during the implementation stage of the research. We also thank Dave Turner of the USDA Forest Service, Rocky Mountain Research Station, for his statistical expertise. NR 52 TC 0 Z9 0 U1 2 U2 21 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD APR PY 2014 VL 60 IS 2 BP 402 EP 408 DI 10.5849/forsci.13-505 PG 7 WC Forestry SC Forestry GA AE6ZB UT WOS:000334145400022 ER PT J AU He, ZQ Chapital, DC Cheng, HN Dowd, MK AF He, Zhongqi Chapital, Dorselyn C. Cheng, Huai N. Dowd, Michael K. TI Comparison of adhesive properties of water- and phosphate buffer-washed cottonseed meals with cottonseed protein isolate on maple and poplar veneers SO INTERNATIONAL JOURNAL OF ADHESION AND ADHESIVES LA English DT Article DE Cottonseed meal; Protein isolate; Wood adhesive; Shear strength; Water resistance ID MODIFIED SOY PROTEIN; WOOD ADHESIVE; 11S GLOBULINS; COMPOSITES; STRENGTH; IMPROVE; FTIR; 7S AB Water- and phosphate buffer (35 mM Na2HPO4/NaH2PO4, pH 7.5)-washed cottonseed meals (abbreviated as WCM and BCM, respectively) could be low-cost and environmentally friendly protein-based adhesives as their preparation does not involve corrosive alkali and acid solutions that are needed for cottonseed protein isolate (CSPI). In this work, we comparatively tested the adhesive shear strength and water resistance properties of WCM, BCM and CSPI on two hard wood veneers, maple and poplar, at press temperatures of 80, 100, 110 and 130 degrees C and a press pressure of 2.8 MPa. The adhesive behaviors of the three adhesives on maple and poplar veneers were similar, although not always identical. Both the adhesive shear strength and water resistance properties of the three adhesives improved with the increase of press temperature from 80 degrees C to over 100 degrees C. However, raising the press temperature improved the water resistance properties of WCM and BCM more than that of CSPI. With a press temperature of 110 degrees C, the adhesive shear strength and water resistance properties of WCM on both maple and poplar veneers were comparable to those of CSPI. Thus, the low-cost preparation of WCM, in comparison to the CSPI preparation, is a strong candidate for substituting synthetic wood adhesives. FT-IR spectra showed higher contents of carbohydrate in WCM and BCM than in CSPI. Cross-linking reactions between carbohydrate and protein at higher press temperatures ( >= 100 degrees C) may have contributed to the improvement of the water resistance properties of WCM and BCM. Published by Elsevier Ltd. C1 [He, Zhongqi; Chapital, Dorselyn C.; Cheng, Huai N.; Dowd, Michael K.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP He, ZQ (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Zhongqi.He@ars.usda.gov OI He, Zhongqi/0000-0003-3507-5013 NR 28 TC 11 Z9 11 U1 0 U2 15 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0143-7496 EI 1879-0127 J9 INT J ADHES ADHES JI Int. J. Adhes. Adhes. PD APR PY 2014 VL 50 BP 102 EP 106 DI 10.1016/j.ijadhadh.2014.01.019 PG 5 WC Engineering, Chemical; Materials Science, Multidisciplinary SC Engineering; Materials Science GA AE6WW UT WOS:000334139700015 ER PT J AU Haven, J Maniscalco, S Bard, S Wathen, L AF Haven, Jackie Maniscalco, Shelley Bard, Sasha Wathen, Lauren TI Building the Next Generation through Leadership SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Editorial Material C1 [Haven, Jackie; Maniscalco, Shelley] USDA, Agr Ctr Nutr Policy & Promot, Nutr Mkt & Commun Div, Alexandria, VA 22302 USA. [Bard, Sasha] Panum Grp, Bethesda, MD USA. RP Haven, J (reprint author), USDA, Agr Ctr Nutr Policy & Promot, Nutr Mkt & Commun Div, Alexandria, VA 22302 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD APR PY 2014 VL 114 IS 4 BP 520 EP 521 DI 10.1016/j.jand.2014.02.008 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AE6DT UT WOS:000334081500002 PM 24656499 ER PT J AU Zoellner, J Connell, C Madson, MB Thomson, JL Landry, AS Molaison, EF Reed, VB Yadrick, K AF Zoellner, Jamie Connell, Carol Madson, Michael B. Thomson, Jessica L. Landry, Alicia S. Molaison, Elaine Fontenot Reed, Vickie Blakely Yadrick, Kathleen TI HUB City Steps: A 6-Month Lifestyle Intervention Improves Blood Pressure among a Primarily African-American Community SO JOURNAL OF THE ACADEMY OF NUTRITION AND DIETETICS LA English DT Article DE Hypertension; Community-based participatory research; Nutrition; Physical activity; Behavior modification ID RANDOMIZED CONTROLLED-TRIALS; DISEASE RISK-FACTORS; PARTICIPATORY RESEARCH; PHYSICAL-ACTIVITY; WALKING INTERVENTION; WEIGHT-REDUCTION; CLINICAL-TRIAL; UNITED-STATES; HYPERTENSION; HEALTH AB The effectiveness of community-based participatory research (CBPR) efforts to address the disproportionate burden of hypertension among African Americans remains largely untested. The objective of this 6-month, noncontrolled, pre-/post-experimental intervention was to examine the effectiveness of a CBPR intervention in achieving improvements in blood pressure, anthropometric measures, biological measures, and diet. Conducted in 2010, this multicomponent lifestyle intervention included motivational enhancement, social support provided by peer coaches, pedometer diary self-monitoring, and monthly nutrition and physical activity education sessions. Of 269 enrolled participants, 94% were African American and 85% were female. Statistical analysis included generalized linear mixed models using maximum likelihood estimation. From baseline to 6 months, blood pressure decreased significantly: mean (+/- standard deviation) systolic blood pressure decreased from 126.0 +/- 19.1 to 119.6 +/- 15.8 mm Hg, P=0.0002; mean diastolic blood pressure decreased from 83.2 +/- 12.3 to 78.6 +/- 11.1 mm Hg, P<0.0001). Sugar intake also decreased significantly as compared with baseline (by approximately 3 tsp; P<0.0001). Time differences were not apparent for any other measures. Results from this study suggest that CBPR efforts are a viable and effective strategy for implementing nonpharmacologic, multicomponent, lifestyle interventions that can help address the persistent racial and ethnic disparities in hypertension treatment and control. Outcome findings help fill gaps. in the literature for effectively translating lifestyle interventions to reach and engage African-American communities to reduce the burden of hypertension. C1 [Zoellner, Jamie] Virginia Polytech Inst & State Univ, Dept Human Nutr Foods & Exercise, Blacksburg, VA 24061 USA. [Connell, Carol; Landry, Alicia S.; Molaison, Elaine Fontenot; Yadrick, Kathleen] Univ So Mississippi, Dept Nutr & Food Syst, Hattiesburg, MS 39406 USA. [Madson, Michael B.] Univ So Mississippi, Dept Psychol, Hattiesburg, MS 39406 USA. [Reed, Vickie Blakely] Univ So Mississippi, Dept Publ Hlth, Hattiesburg, MS 39406 USA. [Thomson, Jessica L.] Louisiana State Univ, USDA ARS, Baton Rouge, LA 70803 USA. RP Zoellner, J (reprint author), Virginia Polytech Inst & State Univ, Dept Human Nutr Foods & Exercise, 1981 Kraft Dr, Blacksburg, VA 24061 USA. EM zoellner@vt.edu FU National Institute on Minority Health and Health Disparities [R24MD002787] FX This research was supported by the National Institute on Minority Health and Health Disparities Award no. R24MD002787. The views expressed are solely those of the authors and do not reflect the official policy or position of the US government. NR 53 TC 8 Z9 8 U1 3 U2 14 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 2212-2672 J9 J ACAD NUTR DIET JI J. Acad. Nutr. Diet. PD APR PY 2014 VL 114 IS 4 BP 603 EP 612 DI 10.1016/j.jand.2013.11.020 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AE6DT UT WOS:000334081500015 PM 24534602 ER PT J AU Garaulet, M Smith, CE Gomez-Abellan, P Ordovas-Montanes, M Lee, YC Parnell, LD Arnett, DK Ordovas, JM AF Garaulet, Marta Smith, Caren E. Gomez-Abellan, Purificacion Ordovas-Montanes, Maria Lee, Yu-Chi Parnell, Laurence D. Arnett, Donna K. Ordovas, Jose M. TI REV-ERB-ALPHA circadian gene variant associates with obesity in two independent populations: Mediterranean and North American SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Circadian; Clock genes; Obesity; REV-ERB-ALPHA-1; Single nucleotide Polymorphism ID ADIPOCYTE DIFFERENTIATION; METABOLIC SYNDROME; FATTY-ACIDS; CLOCK GENE; POLYMORPHISMS; BEHAVIOR; HEALTH; BETA; DIET AB ScopeDespite the solid connection between REV-ERB and obesity, the information about whether genetic variations at this locus may be associated with obesity traits is scarce. Therefore our objective was to study the association between REV-ERB-ALPHA1 rs2314339 and obesity in two independent populations. Methods and resultsParticipants were 2214 subjects from Spanish Mediterranean (n = 1404) and North American (n = 810) populations. Anthropometric, biochemical, dietary, and genotype analyses were performed. We found novel associations between the REV-ERB-ALPHA1 rs2314339 genotype and obesity in two independent populations: in Spanish Mediterranean and North American groups, the frequency of the minor-allele-carriers (AA+ AG) was significantly lower in the abdominally obese group than in those of the nonabdominally obese group (p < 0.05). Minor allele carriers had lower probability of abdominal obesity than noncarriers, and the effect was of similar magnitude for both populations (OR approximate to 1.50). There were consistent associations between REV-ERB-ALPHA1 genotype and obesity-related traits (p < 0.05). Energy intake was not significantly associated with REV-ERB-ALPHA1 rs2314339. However, physical activity significantly differed by genotype. A significant interaction between the REV-ERB-ALPHA1 variant and monounsaturated-fatty-acids (MUFA) intake for obesity was also detected in the Mediterranean population. ConclusionThis new discovery highlights the importance of REV-ERB-ALPHA1 in obesity and provides evidence for the connection between our biological clock and obesity-related traits. C1 [Garaulet, Marta; Gomez-Abellan, Purificacion] Univ Murcia, Fac Biol, Dept Physiol, E-30100 Murcia, Spain. [Garaulet, Marta; Smith, Caren E.; Ordovas-Montanes, Maria; Lee, Yu-Chi; Parnell, Laurence D.; Ordovas, Jose M.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Arnett, Donna K.] Univ Alabama Birmingham, Dept Epidemiol, Birmingham, AL USA. [Ordovas, Jose M.] CNIC, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. RP Garaulet, M (reprint author), Univ Murcia, Fac Biol, Dept Physiol, Campus Espinardo S-N, E-30100 Murcia, Spain. EM garaulet@um.es FU Tomas Pascual and Pilar Gomez-Cuetara Foundations; Spanish Government of Science and Innovation [BFU2011-24720]; Seneca Foundation from the Government of Murcia [15123/PI/10]; National Heart, Lung, and Blood Institute [HL-54776]; National Institute of Diabetes and Digestive and Kidney Diseases [DK075030]; US Department of Agriculture Research [53-K06-5-10, 58-1950-9-001] FX This study was supported by grants from Tomas Pascual and Pilar Gomez-Cuetara Foundations, Spanish Government of Science and Innovation (BFU2011-24720), Seneca Foundation from the Government of Murcia (15123/PI/10). National Heart, Lung, and Blood Institute grants HL-54776, National Institute of Diabetes and Digestive and Kidney Diseases, Grant Number DK075030 and by contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research. MG designed research and wrote the paper. CES wrote the paper. PGA analyzed data and laboratory. MOM wrote the paper. YCL analyzed data and laboratory; LDP selection of the SNPs; DKA population recruitment; JMO designed research and wrote the paper. MG had primary responsibility for final content. All authors read and approved the final manuscript. NR 36 TC 12 Z9 13 U1 0 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD APR PY 2014 VL 58 IS 4 BP 821 EP 829 DI 10.1002/mnfr.201300361 PG 9 WC Food Science & Technology SC Food Science & Technology GA AF2DA UT WOS:000334521600016 PM 24173768 ER PT J AU Mattison, CP Grimm, CC Wasserman, RL AF Mattison, Christopher P. Grimm, Casey C. Wasserman, Richard L. TI In vitro digestion of soluble cashew proteins and characterization of surviving IgE-reactive peptides SO MOLECULAR NUTRITION & FOOD RESEARCH LA English DT Article DE Allergen; Cashew; Digestion; Pepsin; Trypsin ID NUT ANACARDIUM-OCCIDENTALE; ARA H 6; ANA O 2; FOOD ALLERGENS; CONFORMATIONAL EPITOPE; CIRCULAR-DICHROISM; PEANUT ALLERGENS; UNITED-STATES; L.; ANAPHYLAXIS AB ScopeThe stability of food allergens to digestion varies. We characterized the stability of cashew allergens to digestion by pepsin and trypsin and identified IgE-binding epitopes that survive digestion. Methods and resultsThe ability of pepsin and trypsin to digest cashew allergens was assessed with an in vitro digestion model. Samples were evaluated by SDS-PAGE, MS, ELISA, and immunoblotting to compare IgE binding. Increasing amount of protease resulted in greater degradation of higher molecular weight cashew proteins. Among cashew proteins, the 2S albumin, Ana o 3, was most resistant to digestion by both pepsin and trypsin. MS identified digestion resistant Ana o 3 protein fragments that retained reported IgE-binding epitopes. Pretreatment of extracts or purified Ana o 3 with reducing agent increased the sensitivity of Ana o 3 to protease digestion. Circular dichroism revealed the structure of purified Ana o 3 was largely alphahelical and was disrupted following reduction. Ana o 3 reduction followed by protease digestion decreased binding of serum IgE from cashew allergic patients. Our results indicate that the Ana o 3 disulfide bond dependent structure protects the protein from proteolysis. ConclusionAna o 3 is the cashew allergen most likely to survive gastrointestinal digestion intact. C1 [Mattison, Christopher P.; Grimm, Casey C.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Wasserman, Richard L.] Univ Texas Dallas, Dept Pediat, SW Med Sch, Allergy Immunol Res Ctr North Texas, Dallas, TX 75230 USA. RP Mattison, CP (reprint author), ARS, USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM chris.mattison@ars.usda.gov FU U.S. Department of Agriculture-Agricultural Research Service; Allergy/Immunology Research Center of North Texas FX We would like to thank Yvette Bren-Mattison for helpful discussion and critical evaluation of the material presented and Wendy Desormeaux for technical support. This research was supported by funds from the U.S. Department of Agriculture-Agricultural Research Service and the Allergy/Immunology Research Center of North Texas. Mention of trade names, commercial products, or companies in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 36 TC 9 Z9 10 U1 3 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1613-4125 EI 1613-4133 J9 MOL NUTR FOOD RES JI Mol. Nutr. Food Res. PD APR PY 2014 VL 58 IS 4 BP 884 EP 893 DI 10.1002/mnfr.201300299 PG 10 WC Food Science & Technology SC Food Science & Technology GA AF2DA UT WOS:000334521600022 PM 24311529 ER PT J AU He, ZQ Senwo, ZN Zou, HX Tazisong, IA Martens, DA AF He, Z. Q. Senwo, Z. N. Zou, H. X. Tazisong, I. A. Martens, D. A. TI Amino Compounds in Poultry Litter, Litter-Amended Pasture Soils and Grass Shoots SO PEDOSPHERE LA English DT Article DE amino acids; amino sugars; broiler feed; soil nitrogen ID CHROMATOGRAPHY-PULSED AMPEROMETRY; ACID-COMPOSITION; ORGANIC-MATTER; DAIRY MANURE; LAND-USE; NITROGEN; AVAILABILITY; FERTILIZER; DYNAMICS AB Amino compounds (ACs), i.e., amino acids and amino sugars, are the major forms of organic nitrogen (N) in animal manure and soil. To increase our understanding on the effect of long-term poultry litter (PL) application on soil AC pools and turnover, in this study, we determined the contents of 21 ACs in 23 PL samples, 15 soil samples collected from 0-20, 20-40, and 40-60 cm layers of five pasture plots with 0, 5, 10, 15 and 20 years of PL applications, and 5 grass shoot samples grown on these pasture fields. The contents of 21 ACs were simultaneously determined by methanesulfonic acid hydrolysis/extraction and anion chromatography-pulsed amperometry. PL application increased soil total and individual AC contents with a distribution pattern similar to that of AC in PL. The highest AC-N concentrations were observed in the soils with 10- or 15-year PL applications, inconsistent with the order of annual application rates or cumulative applied PL amounts. Application of PL increased the AC contents in grass shoots whereby the highest increase of most ACs was with the shoots from the fields that received PL for 5 years. These observations suggested that both freshly applied and residual PL had contributions to the soil AC-N, and that PL application also accelerated AC-N transformation in soil. C1 [He, Z. Q.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. [Senwo, Z. N.; Tazisong, I. A.] Alabama A&M Univ, Dept Biol & Environm Sci, Normal, AL 35762 USA. [Zou, H. X.] Univ Maine, Dept Chem & Biol Engn, Orono, ME 04469 USA. [Martens, D. A.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. RP He, ZQ (reprint author), USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA. EM Zhongqi.He@ars.usda.gov OI He, Zhongqi/0000-0003-3507-5013 NR 27 TC 3 Z9 3 U1 0 U2 6 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1002-0160 EI 2210-5107 J9 PEDOSPHERE JI Pedosphere PD APR PY 2014 VL 24 IS 2 BP 178 EP 185 PG 8 WC Soil Science SC Agriculture GA AF2RK UT WOS:000334559500003 ER PT J AU Liu, ZF Yoshimura, K Kennedy, CD Wang, XH Pang, SG AF Liu ZhongFang Yoshimura, Kei Kennedy, Casey D. Wang XinHui Pang ShuoGuang TI Water vapor delta D dynamics over China derived from SCIAMACHY satellite measurements SO SCIENCE CHINA-EARTH SCIENCES LA English DT Article DE water vapor delta D; moisture sources; evapotranspiration; droplet evaporation; China ID STABLE-ISOTOPE DATA; TIBETAN PLATEAU; RIVER-BASIN; PRECIPITATION; EVAPORATION; RATIOS; MIDDLE; O-18 AB This study investigates water vapor isotopic patterns and controls over China using high-quality water vapor delta D data retrieved from the Scanning Imaging Absorption Spectrometer for Atmospheric Chartography (SCIAMACHY) observations. The results show that water vapor delta D values on both annual and seasonal time-scales broadly exhibit a continental effect, with values largely decreasing northwestward from coastal lowlands to high-elevation mountainous regions. However, region-specific analysis reveals spatially distinct patterns of water vapor delta D between seasons. In the monsoon domain (e.g., China south of 35A degrees N), depletion in D in the summer and fall seasons is closely tied to monsoon moisture sources (the Indian and Pacific oceans) and subsequent amount effect, but higher delta D values in winter and spring are a result of isotopically-enriched continental-sourced moisture proceeded by less rainout. In contrast, farther inland in China (non-monsoon domain), moisture is derived overwhelmingly from the dry continental air masses and local evaporation, and delta D values are largely controlled by the temperature effect, exhibiting a seasonality with isotopically enriched summer and depleted winter/spring. The observation that the spatial pattern of water vapor delta D is the opposite to that of precipitation delta D in the summer season also suggests that partial evaporation of falling raindrops is a key driver of water vapor isotope in the non-monsoon domain. This study highlights the importance of non-Rayleigh factors in governing water vapor isotope, and provides constraints on precipitation isotope interpretation and modern isotope hydrological processes over China. C1 [Liu ZhongFang; Wang XinHui; Pang ShuoGuang] Tianjin Normal Univ, Tianjin Key Lab Water Resource & Environm, Tianjin 300387, Peoples R China. [Liu ZhongFang; Yoshimura, Kei] Univ Tokyo, Atmosphere & Ocean Res Inst, Kashiwa, Chiba 2778568, Japan. [Kennedy, Casey D.] ARS, USDA, Pasture Syst & Watershed Management Res Unit, East Wareham, MA 02538 USA. RP Liu, ZF (reprint author), Tianjin Normal Univ, Tianjin Key Lab Water Resource & Environm, Tianjin 300387, Peoples R China. EM liuzf406@gmail.com RI Yoshimura, Kei/F-2041-2010 OI Yoshimura, Kei/0000-0002-5761-1561 FU JSPS; National Natural Science Foundation of China [41171022]; Tianjin Municipal Education Commission [20080520] FX This work was supported by the JSPS Fellowship, National Natural Science Foundation of China (Grant No. 41171022) and Tianjin Municipal Education Commission (Grant No. 20080520). We thank two anonymous reviewers for providing comments that have greatly improved the content and readability of the manuscript. NR 42 TC 4 Z9 6 U1 1 U2 16 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1674-7313 EI 1869-1897 J9 SCI CHINA EARTH SCI JI Sci. China-Earth Sci. PD APR PY 2014 VL 57 IS 4 BP 813 EP 823 DI 10.1007/s11430-013-4687-1 PG 11 WC Geosciences, Multidisciplinary SC Geology GA AE7KN UT WOS:000334176800022 ER PT J AU Wang, HZ Ibekwe, AM Ma, JC Wu, LS Lou, J Wu, ZG Liu, RY Xu, JM Yates, SR AF Wang, Haizhen Ibekwe, A. Mark Ma, Jincai Wu, Laosheng Lou, Jun Wu, Zhigang Liu, Renyi Xu, Jianming Yates, Scott R. TI A glimpse of Escherichia coli O157:H7 survival in soils from eastern China SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Escherichia coli O157:H7 (E. coli O157:H7); Survival; Chloroflexi; Amorphous Al2O3; pH ID HEMOLYTIC-UREMIC SYNDROME; COLI O157H7; CONTAMINATED MANURE; STREPTOCOCCUS-SUIS; IRRIGATION WATER; CLAY-MINERALS; UNITED-STATES; O157-H7; O157/H7; EPIDEMIOLOGY AB Escherichia coli O157:H7 (E. coli O157:H7) is an important food-borne pathogen, which continues to be a major public health concern worldwide. It is known that E. coli O157:H7 survive in soil environment might result in the contamination of fresh produce or water source. To investigate how the soils and their properties affect E. coli O157:H7 survival, we studied E. coli O157:H7 survival dynamics in 14 soils collected in eastern China from the warm-temperate zone to subtropical zone. Results showed that E. coli O157:H7 survival as a function of time can be well described by the Weibull model. The calculated t(d) values (survival time to reach the detection limit, 100 colony forming units per gram oven-dried weight of soil) for the test soils were between 1.4 and 25.8 days. A significantly longer survival time (t(d)) was observed in neutral or alkaline soils from north-eastern China (the warm-temperate zone) than that in acidic soils from south-eastern China (the subtropical zone). Distinct E. coli O157:H7 survival dynamics was related to soil properties. Stepwise multiple regression analysis revealed that the t(d) values were significantly enhanced by soil microbial biomass carbon and total nitrogen, but were significantly reduced by amorphous Al2O3 and relative abundance of Chloroflexi. It should pay more attention to E. coli O157:H7 long survival in soils and its potential environmental contamination risk. (c) 2014 Elsevier B.V. All rights reserved. C1 [Wang, Haizhen; Wu, Laosheng; Lou, Jun; Xu, Jianming] Zhejiang Univ, Coll Environm & Nat Resource Sci, Zhejiang Prov Key Lab Subtrop Soil & Plant Nutr, Hangzhou 310058, Zhejiang, Peoples R China. [Wang, Haizhen; Ibekwe, A. Mark; Ma, Jincai; Yates, Scott R.] ARS, USDA, US Salin Lab, Riverside, CA 92507 USA. [Ma, Jincai; Wu, Laosheng] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Wu, Zhigang; Liu, Renyi] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. RP Xu, JM (reprint author), Zhejiang Univ, Coll Environm & Nat Resource Sci, Hangzhou 310058, Zhejiang, Peoples R China. EM jmxu@zju.edu.cn; Scott.Yates@ars.usda.gov RI Ma, Jincai/D-1290-2013; Xu, Jianming/C-8752-2016; Lou, Jun/E-8203-2016 OI Ma, Jincai/0000-0002-0792-0251; Lou, Jun/0000-0001-7872-8200 FU Major Program of National Natural Science Foundation of China [41130532]; National Natural Science Foundation of China [40971255] FX This work was financially supported by the Major Program of National Natural Science Foundation of China (41130532) and the National Natural Science Foundation of China (40971255). NR 49 TC 6 Z9 7 U1 3 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 1 PY 2014 VL 476 BP 49 EP 56 DI 10.1016/j.scitotenv.2014.01.004 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA AE1ZG UT WOS:000333772500006 PM 24463024 ER PT J AU Hanberry, BB Dey, DC He, HS AF Hanberry, Brice B. Dey, Daniel C. He, Hong S. TI The history of widespread decrease in oak dominance exemplified in a grassland-forest landscape SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Fire suppression; Historical forest; Land use; Oak restoration; Savanna; Woodland ID REPEATED PRESCRIBED FIRES; EASTERN UNITED-STATES; ILLINOIS OZARK HILLS; NATURAL REGENERATION; SHELTERWOOD STANDS; HARDWOOD FORESTS; RED MAPLE; QUERCUS; VEGETATION; MISSOURI AB Regionally-distinctive open oak forest ecosystems have been replaced either by intensive agriculture and grazing fields or by denser forests throughout eastern North America and Europe. To quantify changes in tree communities and density in the Missouri Plains, a grassland forest landscape, we used historical surveys from 1815 to 1864 and current surveys from 2004 to 2008. To estimate density for historical communities, we used the Morisita plotless density estimator and applied corrections for surveyor bias. To estimate density for current forests, we used Random Forests, an ensemble regression tree method, to predict densities from known values at plots using terrain and soil predictors. Oak species decreased from 62% of historical composition to 30% of current composition and black and white oaks historically were dominant species across 93% of the landscape and currently were dominant species across 42% of the landscape. Current forest density was approximately two times greater than historical densities, demonstrating loss of savanna and woodlands and transition to dense forest structure. Average tree diameters were smaller than in the past, but mean basal area and stocking remained similar over time because of the increase in density in current forests. Nevertheless, there were spatial differences; basal area and stocking decreased along rivers and increased away from rivers. Oak species are being replaced by other species in the Missouri Plains, similar to replacement throughout the range of Quercus. Long-term commitment to combinations of prescribed burning and silvicultural prescriptions in more xeric sites may be necessary for oak recruitment. Restoration of open oak ecosystems is a time-sensitive issue because restoration will become increasingly costly as oaks are lost from the overstory and the surrounding matrix becomes dominated by non-oak species. (C) 2014 Elsevier B.V. All rights reserved. C1 [Hanberry, Brice B.; He, Hong S.] Univ Missouri, Columbia, MO 65211 USA. [Dey, Daniel C.] Univ Missouri, USDA Forest Serv, No Res Stn, Columbia, MO 65211 USA. RP Hanberry, BB (reprint author), Univ Missouri, 203 Nat Resources Bldg, Columbia, MO 65211 USA. EM hanberryb@missouri.edu OI He, Hong S./0000-0002-3983-2512 FU National Fire Plan, USDA Forest Service FX We thank anonymous reviewers for their time and suggestions. We received funding from the National Fire Plan, USDA Forest Service. NR 77 TC 4 Z9 4 U1 9 U2 42 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD APR 1 PY 2014 VL 476 BP 591 EP 600 DI 10.1016/j.scitotenv.2014.01.048 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA AE1ZG UT WOS:000333772500060 PM 24496032 ER PT J AU Kluber, LA Miller, JO Ducey, TF Hunt, PG Lang, M Ro, KS AF Kluber, Laurel A. Miller, Jarrod O. Ducey, Thomas F. Hunt, Patrick G. Lang, Megan Ro, Kyoung S. TI Multistate assessment of wetland restoration on CO2 and N2O emissions and soil bacterial communities SO APPLIED SOIL ECOLOGY LA English DT Article DE Wetland restoration; CO2; N2O; Soil bacteria; Land use ID COASTAL-PLAIN WETLANDS; CONTRASTING LAND-USES; NITROUS-OXIDE; WATER-QUALITY; MICROBIAL COMMUNITIES; ECOSYSTEM SERVICES; GUT MICROBIOTA; ORGANIC SOILS; DENITRIFICATION; CARBON AB Over the last 200 years, wetlands have been converted to other land uses leading to the loss of approximately 53% of wetlands in the continental United States. In the late 1980's, policies were instated to mitigate further wetland loss through wetland creation and restoration. Restored wetlands provide important ecosystem services, such as filtration of nutrients and wildlife habitat. However, these benefits could be offset by increased greenhouse gas production. We assessed the impact of wetland conversion to agriculture and restoration on CO2 and N2O emissions and microbial communities in three land use types: wetlands with native vegetation (natural); wetlands converted to agricultural management (converted); and restored wetlands (restored). Soil properties varied among land use types. Most notably, soils from restored and converted sites had the lowest C and N, and higher pH. Multivariate analysis of soil properties showed the pocosin wetlands in North Carolina separating from all other locations, regardless of land use. Soil bacterial communities showed a similar trend with communities from North Carolina soils separating from the others with no significant effect of land use or season. Furthermore, land use did not have a significant effect on CO2 or N2O emissions, although there was significant temporal variation in CO2 emissions. These findings indicate that while wetland conversion and restoration may alter some soil properties, these alterations do not appear to be great enough to override the underlying geographic and edaphic influences on soil bacterial communities. Furthermore, wetland restoration did not lead to increased N2O emission at the dates sampled. Published by Elsevier B.V. C1 [Kluber, Laurel A.; Miller, Jarrod O.; Ducey, Thomas F.; Hunt, Patrick G.; Ro, Kyoung S.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC USA. [Lang, Megan] USDA, FS Northern Res Stn, Beltsville, MD 20705 USA. RP Kluber, LA (reprint author), Kluber USDA ARS, 2611 West Lucas St, Florence, SC 29501 USA. EM laurel.kluber@ars.usda.gov OI Miller, Jarrod/0000-0002-5353-233X; Ducey, Thomas/0000-0001-8199-0584; Kluber, Laurel/0000-0002-9302-9413 FU Wetland Component of the USDA National Conservation Effects Assessment Project FX We thank Anthony Shriner, William Brigman, and Katie Lewis for their field and laboratory assistance, Dr. Zaid Abdo for statistical advice, and Drs. Ariel Szogi and Ken Stone for thoughtful discussions on the manuscript content. We also thank the property owners for their cooperation with this study and allowing access to field sites. Funding was provided by the Wetland Component of the USDA National Conservation Effects Assessment Project. Mention of trade or firm names does not constitute an endorsement by the U.S. Department of Agriculture. NR 52 TC 5 Z9 5 U1 11 U2 66 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD APR PY 2014 VL 76 BP 87 EP 94 DI 10.1016/j.apsoil.2013.12.014 PG 8 WC Soil Science SC Agriculture GA AE5DV UT WOS:000334008900011 ER PT J AU Blett, TF Lynch, JA Pardo, LH Huber, C Haeuber, R Pouyat, R AF Blett, Tamara F. Lynch, Jason A. Pardo, Linda H. Huber, Cindy Haeuber, Richard Pouyat, Richard TI FOCUS: A pilot study for national-scale critical loads development in the United States SO ENVIRONMENTAL SCIENCE & POLICY LA English DT Article DE Air quality; Acidification; Nitrogen saturation; Eutrophication; Ecological thresholds ID ATMOSPHERIC NITROGEN DEPOSITION; ACIDIFICATION; USA AB The development and use of critical loads of air pollutant deposition in the U.S. is gaining momentum, and recent research efforts in the U.S. have produced valuable data for calculating critical loads. Critical loads are used to quantify the levels of air pollutants that are expected to impact forest health, soil fertility, aquatic biota condition, and other ecosystem responses. In addition, model refinements for improving critical loads estimates, and maps for illustrating critical loads for acidification and nitrogen saturation and eutrophication resulting from excess nutrient nitrogen, have been developed at various scales. However, prior to the effort described here, no cohesive process existed to provide a national-scale critical loads database and maps as a unified product representing all U.S. ecosystems. The FOCUS (Focal Center Utility Study) Project was initiated to coordinate the development and implementation of a clear, consistent, repeatable process for calculating and mapping critical loads within the U.S. In the FOCUS Phase I Pilot Study, empirical and calculated critical loads data for the U.S. were synthesized from dozens of regional and national-scale monitoring networks, research projects and publically available databases following an approach similar to that used in Europe. The United Nations Economic Commission for Europe (UNECE), through its International Cooperative Programme on Modelling and Mapping of Critical Levels & Loads and Air Pollution Effects, Risks and Trends (ICP-M&M) collects, analyzes and maps critical loads data. Countries participating in the Convention on Long-range Transboundary Air Pollution (CLRTAP) use a Critical Loads "Focal Center" in each country to serve as the point of contact for submitting regional and national-scale critical loads data to the ICP-M&M. One of the purposes of this study was to develop a foundation for interacting with other Focal Centers by assembling critical loads data, creating a database, establishing modeling protocols, and developing infrastructure within the U.S to report and update critical loads on a national scale. Because the U.S. does not currently have an officially designated Focal Center, critical loads data were provided as an informal, unofficial submission to the Coordination Center for Effects (CCE) of the ICP-M&M in March 2011, in the interest of international cooperation and exchange of information on the effects of atmospheric deposition of pollutants on ecosystems. We envision that these data will enable U.S. scientists, land managers, and environmental policymakers to enter into a productive and meaningful dialogue within the US, and also with the international scientific community on methods for estimating, calculating, mapping, interpreting, and refining critical loads for the effects of acidification and excess nutrient nitrogen on terrestrial and aquatic ecosystems. This paper describes the process used to develop national-scale critical loads in the U.S., summarizes the FOCUS Phase I approach and database development effort, and presents some initial national-scale critical loads mapping products. Published by Elsevier Ltd. C1 [Blett, Tamara F.] Natl Pk Serv, Air Resources Div, Lakewood, CO 80225 USA. [Lynch, Jason A.; Haeuber, Richard] US EPA, Clean Air Markets Div, Washington, DC 20040 USA. [Pardo, Linda H.] Univ Vermont, Aiken Ctr, USDA, No Res Stn,Forest Serv, Burlington, VT 05405 USA. [Huber, Cindy] Illinois State Water Survey, Natl Atmospher Deposit Program, Champaign, IL 61820 USA. [Pouyat, Richard] ARS, USDA, R&D, Arlington, VA 22209 USA. RP Blett, TF (reprint author), Natl Pk Serv, Air Resources Div, POB 25287, Lakewood, CO 80225 USA. EM tamara_blett@nps.gov; lynch.jason@epa.gov; lpardo@fs.fed.us; chuber@illinois.edu; haeuber.richard@epa.gov; rpouyat@fs.fed.us NR 41 TC 8 Z9 9 U1 2 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1462-9011 EI 1873-6416 J9 ENVIRON SCI POLICY JI Environ. Sci. Policy PD APR PY 2014 VL 38 BP 225 EP 236 DI 10.1016/j.envsci.2013.12.005 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA AE1HL UT WOS:000333720300021 ER PT J AU Liu, JC Moon, RJ Rudie, A Youngblood, JP AF Liu, Jen-Chieh Moon, Robert J. Rudie, Alan Youngblood, Jeffrey P. TI Mechanical performance of cellulose nanofibril film-wood flake laminate SO HOLZFORSCHUNG LA English DT Article DE cellulose nanofibrils; laminate; phenol-for-maldehyde resin; relative humidity; wood flake ID BACTERIAL CELLULOSE; NANOCOMPOSITES; COMPOSITES; TOUGHNESS; FIBRILS AB Homogeneous and transparent CNF films, fabricated from the (2,2,6,6- tetramethylpiperidin-1-yl) oxyl (TEMPO)-modified CNF suspension, were laminated onto wood flakes (WF) based on phenol-formaldehyde (PF) resin and the reinforcement potential of the material has been investigated. The focus was on the influence of CNF film lamination, relative humidity (RH), heat treatment, and anisotropic properties of WF on the CNF-WF laminate tensile properties (elastic modulus, ultimate tensile strength, strain to failure). Results demonstrated that CNF-WF laminates had improved mechanical performance as compared to the neat WF. In the WF transverse direction, there were gains of nearly 200% in Young's modulus and 300% in ultimate tensile strength. However, in the WF axial direction, the reinforcement effect was minor after PF modification of the wood and the presence of the CNF layers. The effective elastic moduli of the CNF-WF laminates were calculated based on the laminated plate theory, and the calculation in both axial and transverse directions were in agreement with the experimental results. C1 [Liu, Jen-Chieh; Moon, Robert J.; Youngblood, Jeffrey P.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.; Rudie, Alan] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Moon, Robert J.] Purdue Univ, Birck Nanotechnol Ctr, W Lafayette, IN 47907 USA. [Liu, Jen-Chieh] Purdue Univ, W Lafayette, IN 47907 USA. RP Youngblood, JP (reprint author), Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. EM robertmoon@fs.fed.us; jpyoungb@purdue.edu FU US Forest Service-Forest Products Laboratory (FPL) [09-JV-11111137-151] FX The authors would like to thank the US Forest Service-Forest Products Laboratory (FPL) for funding this project (Grant number 09-JV-11111137-151). We are grateful for Rick Reiner and Zhiyong Cai from the FPL for providing the CNF suspensions and wood flakes, respectively. NR 21 TC 3 Z9 3 U1 5 U2 24 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD APR PY 2014 VL 68 IS 3 BP 283 EP 290 DI 10.1515/hf-2013-0071 PG 8 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA AE2JF UT WOS:000333798400004 ER PT J AU Wells, JE Kim, M Bono, JL Kuehn, LA Benson, AK AF Wells, J. E. Kim, M. Bono, J. L. Kuehn, L. A. Benson, A. K. TI MEAT SCIENCE AND MUSCLE BIOLOGY SYMPOSIUM: Escherichia coli O157:H7, diet, and fecal microbiome in beef cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE bovine feces; ecology; Escherichia coli O157:H7 ID DRIED DISTILLERS GRAINS; GASTROINTESTINAL-TRACT; MAXIMUM-LIKELIHOOD; BACTERIAL COMMUNITY; FINISHING DIETS; FEEDLOT CATTLE; PREVALENCE; O157-H7; FECES; DIVERSITY AB Shiga-toxigenic Escherichia coli, such as E. coli O157:H7, are foodborne zoonotic pathogens that can cause severe illness and death in humans. The gastrointestinal tract of ruminant animals has been identified as a primary habitat for E. coli O157:H7 and, in cattle, the hindgut tract appears to be a primary site for colonization. This pathogen has been found in cattle feces, on cattle hides, and in the production environment, and transmission to humans has occurred as a result of consumption of contaminated ground beef, water, and produce. Interventions to reduce the pathogen at beef harvest have significantly reduced the occurrence of the pathogen, but outbreaks and recalls due to the pathogen still occur for beef products. Interventions in the feedyard before harvest have had little success, but critical control points for implementing interventions are limited compared with the beef abattoir. The percentage of animals shedding E. coli O157:H7 in the feces can be highly variable from pen to pen, and the levels in the feces can vary from animal to animal. Animals colonized and shedding E. coli O157:H7 at high levels are a small fraction of animals in a pen but are important source for transferring the pathogen amongst the penmates. Recent research has indicated that diet may greatly influence the shedding of E. coli O157:H7. In addition, diet can influence the microbiota composition of the feces. However, little is known about the interaction between the indigenous microbiota and fecal shedding of E. coli O157:H7. Understanding the influence of indigenous microbiota on the colonization and shedding of E. coli O157:H7 will provide a potential avenue for intervention in the preharvest production environment not yet exploited. C1 [Wells, J. E.; Kim, M.; Bono, J. L.; Kuehn, L. A.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Benson, A. K.] Univ Nebraska, Dept Food Sci & Technol, Lincoln, NE 68583 USA. RP Wells, JE (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM Jim.Wells@ars.usda.gov NR 81 TC 1 Z9 2 U1 6 U2 39 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2014 VL 92 IS 4 BP 1345 EP 1355 DI 10.2527/jas.2013-7282 PG 11 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD9JT UT WOS:000333581900008 PM 24492542 ER PT J AU Callaway, TR Edrington, TS Nisbet, DJ AF Callaway, T. R. Edrington, T. S. Nisbet, D. J. TI MEAT SCIENCE AND MUSCLE BIOLOGY SYMPOSIUM: Ecological and dietary impactors of foodborne pathogens and methods to reduce fecal shedding in cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE foodborne diseases; interventions; pathogens; preharvest ID ESCHERICHIA-COLI O157-H7; COMMERCIAL FEEDLOT CATTLE; DRIED DISTILLERS GRAINS; PROTEINS-BASED VACCINE; III SECRETED PROTEINS; DIRECT-FED MICROBIALS; LACTIC-ACID BACTERIA; COMPETITIVE-EXCLUSION; SALMONELLA-ENTERICA; SODIUM-CHLORATE AB Pathogenic bacteria can live asymptomatically within and on cattle and can enter the food chain but also can be transmitted to humans by fecal or direct animal contact. Reducing pathogenic bacterial incidence and populations within live cattle represents an important step in improving food safety. A broad range of preslaughter intervention strategies are being developed, which can be loosely classified as 1) directly antipathogen strategies, 2) competitive enhancement strategies (that use the microbiome's competitive nature against pathogens), and 3) animal management strategies. Included within these broad categories are such diverse methods as vaccination against foodborne pathogens, probiotics and prebiotics, bacterial viruses (i.e., bacteriophages), sodium chlorate feeding, and dietary and management changes that specifically alter the microbiome. The simultaneous application of 1 or more preharvest strategies has the potential to reduce human foodborne illnesses by erecting multiple hurdles preventing entry into humans. However, economic factors that govern producer profitability must be kept in mind while improving food safety. C1 [Callaway, T. R.; Edrington, T. S.; Nisbet, D. J.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. RP Callaway, TR (reprint author), ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. EM todd.callaway@ars.usda.gov NR 152 TC 1 Z9 1 U1 2 U2 18 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2014 VL 92 IS 4 BP 1356 EP 1365 DI 10.2527/jas.2013-7308 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD9JT UT WOS:000333581900009 PM 24669007 ER PT J AU McDaneld, TG Kuehn, LA Thomas, MG Pollak, EJ Keele, JW AF McDaneld, T. G. Kuehn, L. A. Thomas, M. G. Pollak, E. J. Keele, J. W. TI Deletion on chromosome 5 associated with decreased reproductive efficiency in female cattle SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE bovine; copy number variation; reproductive efficiency ID AFFECTING TWINNING RATE; BOS-TAURUS; GENOME AB Reproductive efficiency is arguably the most economically important trait in commercial beef cattle production, as failure to achieve pregnancy reduces the number of calves marketed per cow exposed to breeding. Identification of variation in the genome with predictive merit for reproductive success would facilitate accurate prediction of daughter pregnancy rate in sires enabling effective selection of bulls producing daughters with improved fertility. Copy number variation (CNV) is one form of structural variation that results in abnormal copies of DNA in the genome. Our lab previously reported a region between 25 and 70 Mb on chromosome 5 associated with reproductive efficiency. To further evaluate this region for genomic variations such as CNV, we initially applied a genomewide association approach based on the >700,000 SNP marker assay and PennCNV analysis to 68 Bos indicus cross females from a large commercial ranch in central Florida. A genomic segment located on chromosome 5, spanning the region of approximately 58.37 to 58.44 Mb (Bovine UMD3.1 assembly) was identified as containing a deletion associated with decreased reproductive efficiency. To verify this deletion, a quantitative real-time PCR test was developed to evaluate additional females in the central Florida and U. S. Meat Animal Research Center (USMARC) populations. The frequency of the homozygous deletion was 7% (23/319) in the central Florida population (Bos indicus cross) for females with 2 consecutive failed breeding seasons and 0% in the USMARC population (predominantly Bos taurus) of low reproductive females. To date, we have not identified the deletion in Bos taurus cattle, suggesting that the deletion is specific to Bos indicus cattle. From these data, we have identified a deletion on chromosome 5 associated with reproductive efficiency in Bos indicus-influenced cattle. C1 [McDaneld, T. G.; Kuehn, L. A.; Pollak, E. J.; Keele, J. W.] ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Thomas, M. G.] Colorado State Univ, Dept Anim Sci, Ft Collins, CO 80523 USA. RP McDaneld, TG (reprint author), ARS, USDA, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM tara.mcdaneld@ars.usda.gov FU USDA-AFRI [2008-35205-18751] FX Financial support provided in part by USDA-AFRI (grant no. 2008-35205-18751) for collection of DNA samples. NR 20 TC 3 Z9 3 U1 3 U2 7 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2014 VL 92 IS 4 BP 1378 EP 1384 DI 10.2527/jas.2013-6821 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD9JT UT WOS:000333581900011 PM 24492568 ER PT J AU Blackburn, HD Plante, Y Rohrer, G Welch, EW Paiva, SR AF Blackburn, H. D. Plante, Y. Rohrer, G. Welch, E. W. Paiva, S. R. TI Impact of genetic drift on access and benefit sharing under the Nagoya Protocol: The case of the Meishan pig SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE genetic diversity; genetic drift; Meishan; Nagoya protocol; Sus scrofa ID EFFECTIVE POPULATION-SIZE; RESOURCES; PROGRAM; BREEDS AB Genetic drift (GD) randomly impacts small breeds and imported populations. Therefore, it can impact policies that affect conservation of animal genetic resources. This paper evaluates GD for a population of Meishan pigs imported into the United States and explores the ramifications of GD on access and benefit sharing of genetic resources under the Nagoya Protocol (NP) of the United Nations' Convention on Biological Diversity. The NP was motivated by concerns about fair and equitable benefit sharing of genetic resources across life forms. In this experiment, 35 microsatellite markers were used to quantify the level of GD that occurred between Meishan pigs (Meishan-China; n = 22) imported into the United States in the late 1980s and contemporary Meishan (Meishan-US; n = 42), which have been randomly bred since importation. The Meishan-US consisted of 2 subpopulations (Meishan-MARC and Meishan-ISU). Five other breeds were also included in the analysis to serve as reference populations: Fengjing and Minzhu, which were imported with Meishan-China, and Duroc, Berk-shire, and Yorkshire from the United States. Mean shift in allele frequency was 0.11 (SE = 0.019) due to GD for Meishan-US vs. Meishan-China with some loci having changed allele frequencies by greater than 0.20. Principle coordinate analysis confirmed divergence among the Meishan populations. Model-based clustering tended to place the U.S. and Chinese breeds into 2 distinct clusters, likely due to differences in allele frequencies between U.S. and Chinese breeds. Contemporary Meishan-US has become differentiated from the original imported animals due to GD. Attributing future performance of Meishan-US to Meishan-China, as set forth by NP, is problematic due to GD. As an imported breed becomes established there will be an increasing number of breeders who may have different selection goals and private treaty contracts will govern the exchange of stock between them. Therefore, considering biological phenomena and social dynamics simultaneously draws into question whether a rigorous access and benefit sharing protocol as envisioned in the NP will be operational. C1 [Blackburn, H. D.] ARS, Natl Anim Germplasm Program, Natl Ctr Genet Resources Preservat, USDA, Ft Collins, CO 80521 USA. [Plante, Y.] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK, Canada. [Rohrer, G.] ARS, US Meat Anim Res Ctr, USDA, Clay Ctr, NE 68935 USA. [Welch, E. W.] Univ Illinois, Chicago, IL 60607 USA. [Paiva, S. R.] EMBRAPA Secretaria Relacoes Int, Secretariat Int Affairs, Brasilia, DF, Brazil. RP Blackburn, HD (reprint author), ARS, Natl Anim Germplasm Program, Natl Ctr Genet Resources Preservat, USDA, Ft Collins, CO 80521 USA. EM Harvey.blackburn@ars.usda.gov RI Welch, Eric/D-5097-2015 OI Welch, Eric/0000-0003-1902-0370 NR 28 TC 3 Z9 3 U1 0 U2 16 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2014 VL 92 IS 4 BP 1405 EP 1411 DI 10.2527/jas.2013-7274 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD9JT UT WOS:000333581900014 PM 24496834 ER PT J AU Egert, AM Kim, DH Schrick, FN Harmon, DL Klotz, JL AF Egert, A. M. Kim, D. H. Schrick, F. N. Harmon, D. L. Klotz, J. L. TI Dietary exposure to ergot alkaloids decreases contractility of bovine mesenteric vasculature SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE bovine; ergot alkaloid; mesenteric artery and vein; midgut; tall fescue; vasoconstriction ID INFECTED TALL FESCUE; LATERAL SAPHENOUS-VEIN; PROTEIN-COUPLED RECEPTORS; BLOOD-FLOW; DOPAMINE-RECEPTORS; ANTERIOR-PITUITARY; EPICHLOE-TYPHINA; PROLACTIN; CATTLE; ERGOVALINE AB Ergot alkaloids are hypothesized to cause vasoconstriction in the midgut, and prior exposure may affect the vasoactivity of these compounds. The objectives of this study were to profile vasoactivity of ergot alkaloids in bovine mesenteric artery (MA) and vein (MV) and determine if previous exposure to endophyte-infected tall fescue seed affected vasoactivity of ergocryptine (ERP), ergotamine (ERT), ergocristine (ERS), ergocornine (ERO), ergonovine (ERN), lysergic acid (LSA), ergovaline-containing tall fescue seed extract (EXT), and 5-hydroxytryptamine (5HT; serotonin). Ruminally cannulated Angus steers (n = 12; BW = 547 +/- 31 kg) were paired by weight and randomly assigned to 6 blocks. Steers were ruminally dosed daily with 1 kg of either endophyte-infected (E+; 4.45 mg ergovaline/kg DM) or endophyte-free (E-; 0 mg ergovaline/kg DM) tall fescue seed for 21 d before slaughter. Branches of MA and MV supporting the cranial portion of the ileum were collected after slaughter on d 22, placed in a modified Krebs-Henseleit buffer on ice, cleaned, sectioned, and mounted in a multimyograph chamber. Contractile response was normalized to a maximum KCl response. Inner diameter (P = 0.04) and outer diameter (P = 0.02) of MA were smaller for E+ steers than E-steers. Maximum contractile responses to 120 mM KCl were not different between seed treatments in MA (P = 0.33; E-: 2.67 +/- 0.43 g; E+: 3.33 +/- 0.43 g) or MV (P = 0.26; E-: 2.01 +/- 0.18 g; E+: 1.81 +/- 0.18 g). Steers receiving E+ had a smaller (P < 0.01) MA contractile response than E-steers to ERP, ERT, ERS, ERO, ERN, EXT, and 5HT. Steers receiving E+ had a smaller (P < 0.05) MV contractile response than E-steers to ERP, ERT, ERS, ERN, EXT, and 5HT. Lysergic acid failed to induce a contractile response in MA and MV. The contractile response in MA and MV of E-steers produced by 5HT was very large. The EXT was the most potent (P < 0.05) agonist in MV and MA of E+ steers. These data showed that ergot alkaloids were vasoactive in the bovine midgut, and steers exposed to E+ had diminished contractility to some ergot alkaloids in small intestinal vasculature. The findings of this study suggest that dietary exposure to ergot alkaloids has the potential to alter nutrient absorption from the midgut by decreasing blood flow to and from the midgut due to vasoconstriction. C1 [Egert, A. M.; Kim, D. H.; Harmon, D. L.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Schrick, F. N.] Univ Tennessee, Knoxville, TN 37996 USA. [Klotz, J. L.] Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. RP Klotz, JL (reprint author), Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. EM james.klotz@ars.usda.gov NR 47 TC 7 Z9 7 U1 1 U2 8 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD APR PY 2014 VL 92 IS 4 BP 1768 EP 1779 DI 10.2527/jas.2013-7141 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA AD9JT UT WOS:000333581900052 PM 24492572 ER PT J AU Wang, YS Bradford, SA Simunek, J AF Wang, Yusong Bradford, Scott A. Simunek, Jiri TI Estimation and upscaling of dual-permeability model parameters for the transport of E. coli D21g in soils with preferential flow SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Microorganism; Transport; Preferential flow; Dual-permeability model ID HETEROGENEOUS POROUS-MEDIA; SOLUTE TRANSPORT; MACROPOROUS SOIL; VADOSE ZONE; WATER-FLOW; STOCHASTIC-ANALYSIS; SOLUTION CHEMISTRY; FECAL BACTERIA; POROSITY MODEL; IONIC-STRENGTH AB Dual-permeability models are increasingly used to quantify the transport of solutes and microorganisms in soils with preferential flow. An ability to accurately determine the model parameters and their variation with preferential pathway characteristics is crucial for predicting the transport of microorganisms in the field. The dual-permeability model with optimized parameters was able to accurately describe the transport of E. con D21g in columns with artificial macropores of different configurations and lengths at two ionic strength levels (1 and 20 mM NaCI). Correlations between the model parameters and the structural geometry of the preferential flow path were subsequently investigated. Decreasing the macropore length produced a decrease in the apparent saturated hydraulic conductivity of the macropore domain and an increase in the mass transfer between the macropore and matrix domains. The mass transfer coefficient was also found to be dependent on the configuration of the preferential flow pathway. A linear superposition approach was used to estimate field-scale preferential transport behavior for hypothetical fields with different amounts and configurations of macropores. Upscaling procedures were numerically investigated to predict this field-scale transport behavior from column-scale parameters. The upscaling method provided a satisfactory prediction of the field results under the tested scenarios. This information will be useful in assessing the risks of microbial transport due to preferential flow. (c) 2014 Elsevier B.V. All rights reserved. C1 [Wang, Yusong; Simunek, Jiri] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Bradford, Scott A.] ARS, USDA, Salin Lab, Riverside, CA USA. RP Wang, YS (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. EM Ywang032@ucr.edu FU USDA, ARS [NP 214]; Multistate Research Funded project [W-2188] FX This research was supported in part by the USDA, ARS, NP 214, and Multistate Research Funded project W-2188. The USDA is an equal opportunity provider and employer. NR 67 TC 9 Z9 9 U1 5 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 EI 1873-6009 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD APR PY 2014 VL 159 BP 57 EP 66 DI 10.1016/j.jconhyd.2014.01.009 PG 10 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA AE6FG UT WOS:000334085400006 PM 24589387 ER PT J AU Xu, J Shi, CL Song, MH Xu, XB Yang, PY Paoli, G Shi, XM AF Xu, Jie Shi, Chunlei Song, Minghui Xu, Xuebin Yang, Puyu Paoli, George Shi, Xianming TI Phenotypic and Genotypic Antimicrobial Resistance Traits of Foodborne Staphylococcus aureus Isolates from Shanghai SO JOURNAL OF FOOD SCIENCE LA English DT Article DE antibiotic resistance; food; resistance gene; Staphylococcus aureus; subtyping ID SPECTRUM BETA-LACTAMASES; FECAL ESCHERICHIA-COLI; ANTIBIOTIC-RESISTANCE; CHICKEN MEAT; GENES; BACTERIA; IDENTIFICATION; STRAINS; GYRA; FLUOROQUINOLONES AB Staphylococcus aureus is a recognized pathogen in humans, which causes nosocomial infections and food poisoning. The transmission of antibiotic resistant S. aureus (ARSA), especially methicillin-resistant S. aureus, between food products and humans has become a serious problem. Hence, it is necessary to monitor S. aureus through the food supply chain. In this study, the disk diffusion method and epsilometer test were performed to determine the prevalence of ARSA in 78 foodborne isolates using 18 antibiotics. The highest resistance frequency was found for penicillin G (74.4%), followed by erythromycin (59.0%) and clindamycin (44.9%), whereas no vancomycin-resistant isolates were found. The 78 isolates could be subtyped into 31 resistance profiles and 11 clusters based on their antimicrobial susceptibility. Furthermore, Polymerase chain reaction (PCR) screening for the presence of 13 genes conferring antibiotic resistance was conducted. The presence of resistance genes was relatively high: bla(TEM) (80.8%), ermB (41.0%), grlA (38.5%), ermC (35.9%), and aac6'/aph2 (35.9%). The incidence of antibiotic resistance was significantly correlated to food types (p = 0.018), with isolates from meat and raw milk more resistant to antibiotics than those from frozen food and vegetables. Practical Application The antibiotic resistance of S. aureus has become a serious concern in foodstuffs and varies in different foods. Raw milk and meat have more resistant isolates than those from frozen food and vegetables. Resistance profiles and Ward's minimum variance method can be used to subtype isolates in different clusters. C1 [Xu, Jie; Shi, Chunlei; Song, Minghui; Yang, Puyu; Shi, Xianming] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. [Xu, Jie; Shi, Chunlei; Song, Minghui; Yang, Puyu; Shi, Xianming] Shanghai Jiao Tong Univ, Dept Food Sci & Technol, Sch Agr & Biol, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China. [Xu, Xuebin] Shanghai Municipal Ctr Dis Control & Prevent, Microbiol Lab, Shanghai 200336, Peoples R China. [Paoli, George] ARS, USDA MOST Joint Res Ctr Food Safety, Mol Characterizat Foodborne Pathogens Res Unit, USDA,ERRG, Wyndmoor, PA 19038 USA. RP Shi, XM (reprint author), Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200240, Peoples R China. EM xmshi@sjtu.edu.cn FU Ministry of Science and Technology of China [2012AA101601, 2012BAK17B10, 2012BAD29B02]; Ministry of Education of China [20120073110052] FX This study was supported by Ministry of Science and Technology of China (2012AA101601, 2012BAK17B10 and 2012BAD29B02) and Ministry of Education of China (20120073110052). NR 59 TC 6 Z9 8 U1 1 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-1147 EI 1750-3841 J9 J FOOD SCI JI J. Food Sci. PD APR PY 2014 VL 79 IS 4 BP M635 EP M642 DI 10.1111/1750-3841.12405 PG 8 WC Food Science & Technology SC Food Science & Technology GA AE3OO UT WOS:000333886000028 PM 24689704 ER PT J AU Pinheiro, P Bereman, MS Burd, J Pals, M Armstrong, S Howe, KJ Thannhauser, TW MacCoss, MJ Gray, SM Cilia, M AF Pinheiro, Patricia Bereman, Michael S. Burd, John Pals, Melissa Armstrong, Scott Howe, Kevin J. Thannhauser, Theodore W. MacCoss, Michael J. Gray, Stewart M. Cilia, Michelle TI Evidence of the Biochemical Basis of Host Virulence in the Greenbug Aphid, Schizaphis graminum (Homoptera: Aphididae) SO JOURNAL OF PROTEOME RESEARCH LA English DT Article DE aphid; biotype; vector biology; insect-plant interactions; host virulence; Schizaphis graminum; greenbug; DIGE; LC-MS/MS ID CHROMATIN-REMODELING COMPLEX; DNA SEQUENCE DIVERGENCE; YELLOW DWARF VIRUS; PEA APHID; ACYRTHOSIPHON-PISUM; RESISTANCE GENES; LUTEOVIRUS TRANSMISSION; PROTEIN IDENTIFICATION; SEXUAL REPRODUCTION; BACTERIAL SYMBIONTS AB Biotypes of aphids and many other insect pests are defined based on the phenotypic response of host plants to the insect pest without considering their intrinsic characteristics and genotypes. Plant breeders have spent considerable effort developing aphid-resistant, small-grain varieties to limit insecticide control of the greenbug, Schizaphis graminum. However, new S. graminum biotypes frequently emerge that break resistance. Mechanisms of virulence on the aphid side of the plant insect interaction are not well understood. S. graminum biotype H is highly virulent on most small grain varieties. This characteristic makes biotype H ideal for comparative proteomics to investigate the basis of biotype virulence in aphids. In this study, we used comparative proteomics to identify protein expression differences associated with virulence. Aphid proteins involved in the tricarboxylic acid cycle, immune system, cell division, and antiapoptosis pathways were found to be up-regulated in biotype H relative to other biotypes. Proteins from the bacterial endosymbiont of aphids were also differentially expressed in biotype H. Guided by the proteome results, we tested whether biotype H had a fitness advantage compared with other S. graminum biotypes and found that biotype H had a higher reproductive fitness as compared with two other biotypes on a range of different wheat germplasms. Finally, we tested whether aphid genetics can be used to further dissect the genetic mechanisms of biotype virulence in aphids. The genetic data showed that sexual reproduction is a source of biotypic variation observed in S. graminum. C1 [Pinheiro, Patricia] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Pinheiro, Patricia] Embrapa Rice & Beans, BR-75375000 Santo Antonio De Goias, Go, Brazil. [Bereman, Michael S.; MacCoss, Michael J.] Univ Washington, Dept Genome Sci, Seattle, WA 98195 USA. [Burd, John; Pals, Melissa; Armstrong, Scott] USDA ARS, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Howe, Kevin J.; Thannhauser, Theodore W.; Gray, Stewart M.; Cilia, Michelle] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Gray, Stewart M.; Cilia, Michelle] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Cilia, Michelle] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. RP Cilia, M (reprint author), USDA ARS, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM mlc68@cornell.edu RI Pinheiro, Patricia/F-9364-2014 OI Pinheiro, Patricia/0000-0003-0461-1821 FU USDA NRI [1907-22000-018-13]; NSF-BREAD [1109989]; Embrapa Graduate Student Fellowship FX We thank Mathieu Rolland (Cornell) for assistance with data analysis, Dawn Smith (Cornell), Jason Ingram (USDA, ARS), and Michelle Sweeney (BTI) for assistance with the aphids, Tara Fish (USDA) for technical assistance, Tom Hammond (Cornell) for care of the plants, Stacy DeBlasio (USDA, ARS) for helpful discussions, Monica Franciscus (Boyce Thompson Institute) for editorial comments on the manuscript, and Claire Smith (Cornell) for assistance with the graphical abstract. The work was graciously funded by USDA NRI project 1907-22000-018-13, NSF-BREAD project 1109989, and an Embrapa Graduate Student Fellowship to Patricia Pinheiro. NR 90 TC 7 Z9 7 U1 2 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1535-3893 EI 1535-3907 J9 J PROTEOME RES JI J. Proteome Res. PD APR PY 2014 VL 13 IS 4 BP 2094 EP 2108 DI 10.1021/pr4012415 PG 15 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA AE5GS UT WOS:000334016400028 PM 24588548 ER PT J AU Hooley, CG Barrows, FT Paterson, J Sealey, WM AF Hooley, Christopher G. Barrows, Frederic T. Paterson, John Sealey, Wendy M. TI Examination of the Effects of Dietary Protein and Lipid Levels on Growth and Stress Tolerance of Juvenile Tilapia, Oreochromis niloticus SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID COMMON CARP; FRESH-WATER; CORTISOL; PLASMA; RESPONSES; AUREUS AB Tilapia is the second most consumed farmed fish after carp and the most widely grown farmed fish. However, significant price increases in ingredients in recent years threaten profitability of the industry because dietary costs are approximately 60% of production costs. Therefore, the objective of this study was to optimize dietary protein and lipid levels of juvenile tilapia cultured in high-intensity recirculating-water system and assess dietary effects on stress tolerance. To achieve this objective, a factorial treatment design with diets formulated to contain three levels of dietary protein (28, 32, and 36%) and three levels of dietary lipid (3, 6, and 9%) was employed. Juvenile tilapia (34.5 +/- 0.4 g initial weight) were randomly assigned to each of 24 tanks and fed one of the nine diets, three feedings per day to apparent satiation, 6 d/wk for 12 wk. Fish were weighed and counted every 3 wk and feed consumed recorded weekly. Tilapia weight gain, feed conversion, feed intake, and proximate composition were significantly affected by diet. Following the feeding trial, tilapia were subjected to a 24 h simulated live haul where fish were stocked into a static water system at 0.24 kg/L of water. Glucose, lactate, and cortisol measurements were taken at time 0, 24, and 72 h after fish were placed into hauling containers. There was significant effects (P < 0.05) of crude lipid and crude protein on blood chemistry parameters. These data indicate that practical diet formulations can be improved to enhance growth rates, production of tilapia, and stress response during live hauls. C1 [Hooley, Christopher G.; Sealey, Wendy M.] USFWS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Hooley, Christopher G.; Paterson, John] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59715 USA. [Barrows, Frederic T.] ARS, USDA, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. RP Sealey, WM (reprint author), USFWS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. FU Western Regional Aquaculture Center from the USDA National Institute of Food and Agriculture [2010-38500-21758] FX The authors wish to thank David Brock (Rangen Aquafeeds) and Chris Nelson (Silver Cup Feeds) for their guidance in practical tilapia formulations, Jason Frost and Andy Lybeck for their assistance with diet manufacturing, Matt Toner, Cal Fraser, Jason Ilgen, and Blake Hauptman for their assistance in fish culture and sampling, and Aaron Nistler, Eli Cureton, and T. Gibson Gaylord for their assistance with laboratory analyses. This project was supported by Western Regional Aquaculture Center grant no. 2010-38500-21758 from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USFWS. NR 28 TC 0 Z9 0 U1 1 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD APR PY 2014 VL 45 IS 2 BP 115 EP 126 DI 10.1111/jwas.12110 PG 12 WC Fisheries SC Fisheries GA AE3SU UT WOS:000333898300003 ER PT J AU Peterson, BC Bosworth, BG Li, MHH Beltran, R Santos, GA AF Peterson, Brian C. Bosworth, Brian G. Li, Menghe H. Beltran, Ruben, Jr. Santos, Goncalo A. TI Assessment of a Phytogenic Feed Additive (Digestarom P.E.P. MGE) on Growth Performance, Processing Yield, Fillet Composition, and Survival of Channel Catfish SO JOURNAL OF THE WORLD AQUACULTURE SOCIETY LA English DT Article ID ANTIBACTERIAL ACTIVITY AB We investigated the effects of a phytogenic feed additive (Digestarom (R) P.E.P. MGE) on growth performance, processing yield, fillet composition, and survival of pond-raised channel catfish. Fifteen 0.4 ha ponds were stocked with 14,820 catfish (126 g/fish) per ha. Fish in control ponds were fed a 32% crude protein commercial floating diet whereas fish in test ponds were fed the same diet supplemented with Digestarom P.E.P. MGE at 200 g/ton. In a second study, ten 0.04 ha ponds were stocked at a similar density with fish that averaged 68 g/fish. At the end of the 6-mo study, there was no significant difference in the amount of feed fed or the amount of weight gained between the control and Digestarom P.E.P. MGE fed fish. Food conversion ratio, net yield, and survival were also similar between the two groups. Carcass, fillet, and nugget yield were similar. Fillet proximate analysis revealed that fillet fat was lower (P < 0.01) whereas fillet protein tended to be a little higher (P < 0.10) in treated fish. In conclusion, there was a significant reduction in the amount of fillet fat in Digestarom P.E.P. MGE fed fish. Improved fillet composition (higher protein and lower lipid) is of commercial importance. C1 [Peterson, Brian C.; Bosworth, Brian G.] USDA ARS, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. [Li, Menghe H.] Mississippi State Univ, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. [Beltran, Ruben, Jr.] BIOMIN Holding GmbH, San Antonio, TX 78213 USA. [Santos, Goncalo A.] BIOMIN Holding GmbH, A-3130 Herzogenburg, Austria. RP Peterson, BC (reprint author), USDA ARS, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, POB 38, Stoneville, MS 38776 USA. NR 16 TC 4 Z9 4 U1 1 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0893-8849 EI 1749-7345 J9 J WORLD AQUACULT SOC JI J. World Aquacult. Soc. PD APR PY 2014 VL 45 IS 2 BP 206 EP 212 DI 10.1111/jwas.12103 PG 7 WC Fisheries SC Fisheries GA AE3SU UT WOS:000333898300012 ER PT J AU Cushman, RA McNeel, AK Freetly, HC AF Cushman, R. A. McNeel, A. K. Freetly, H. C. TI The impact of cow nutrient status during the second and third trimesters on age at puberty, antral follicle count, and fertility of daughters SO LIVESTOCK SCIENCE LA English DT Article DE Antral follicle count; Beef replacement heifers; Fetal programming; Puberty ID GONADOTROPIN GENE-EXPRESSION; BEEF-CATTLE; HEIFER CALVES; ESTROUS-CYCLE; GESTATION; FEMALES; GROWTH; POLYMORPHISM; PERFORMANCE; MORPHOLOGY AB Fluctuating feed resources to beef cows across the production cycle is a proven method for decreasing input costs; however, limiting nutrients during late gestation have been demonstrated to decrease ovarian follicle numbers in female offspring in some studies. We hypothesize that limiting nutrients to mature (>= 3 yr) crossbred beef cows during the second and third trimesters would result in daughters that would have decreased follicle numbers detectable by ultrasonography as yearlings. Over four breeding seasons, pregnant beef cows (n=397) were assigned to either Low (L), Moderate (M) or High (H) nutrient intake during the second or third trimester, resulting in four dietary treatment groups (L-H, L-L, M-H, and M-M). Heifers (n=416) born to these cows were weighed at weaning and moved to a dry lot where they were monitored for behavioral estrus with the aid of heat detection patches. Two weeks before their first breeding season, heifers were submitted for ultrasonographic examination of their ovaries to determine antral follicle numbers. Heifers were placed with bulls for 60 d and pregnancy status was determined 45 d after the bulls were removed. Growth and reproductive traits were analyzed using the MIXED Procedure of SAS with maternal diet and year as fixed effects. Maternal dietary intake did not affect heifer growth rates, age at puberty, or antral follicle counts (P >= 0.40). However, an increased proportion of the heifers born to dams fed a high nutrient diet during the third trimester (L-H or M-H) calved in the first 21 d of their first calving season (P=0.004). Antral follicle counts detectable by ultrasonography at yearling pre-breeding examination were greater in heifers that calved during the first 21 d of their first calving season (P=0.02); however, these heifers did not differ in age at puberty (P=0.60). From this study, we conclude that: (1) limiting nutrient intake during late gestation in mature (>= 3 yr) beef cows does not influence the ovarian reserve or reproductive performance of daughters; (2) increasing maternal nutrient intake during and 1/4 Red Poll) ranging from 3 to 10 yr in age and determined to be pregnant by ultrasonographic examination were used across 4 yr. Cows remained on the study as long as they remained in the production herd and were determined to be pregnant. Cows were rotated among nutritional treatments during the years that they were on the study so that they did not repeat the same nutritional treatment while on study. C1 [Cushman, R. A.; McNeel, A. K.; Freetly, H. C.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Cushman, RA (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, POB 166,State Spur 18D, Clay Ctr, NE 68933 USA. EM bob.cushman@ars.usda.gov FU ARS Research Projects [5438-31000-093-00D, 5438-31000-092-00D] FX The authors gratefully acknowledge G. Hays, C. Engle and USMARC Cattle Operations for care and handling of the cows and heifers; M. Wilford and K. Beavers for aid with data collection; L. Parnell for manuscript preparation; and D. Light, and L. Larsen for data management. The authors also thank Drs. Rick Funston and Allison Meyer for expert critical review of the manuscript. This research was funded by ARS Research Projects 5438-31000-093-00D (RAC and AKM) and 5438-31000-092-00D (HCF). NR 22 TC 8 Z9 9 U1 4 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD APR PY 2014 VL 162 BP 252 EP 258 DI 10.1016/j.livsci.2014.01.033 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA AE2CE UT WOS:000333780100030 ER PT J AU DeGloria, SD Beaudette, DE Irons, JR Libohova, Z O'Neill, PE Owens, PR Schoeneberger, PJ West, LT Wysocki, DA AF DeGloria, Stephen D. Beaudette, Dylan E. Irons, James R. Libohova, Zamir O'Neill, Peggy E. Owens, Philip R. Schoeneberger, Philip J. West, Larry T. Wysocki, Douglas A. TI Emergent Imaging and Geospatial Technologies for Soil Investigations SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Editorial Material ID PASSIVE SMAP MISSION C1 [DeGloria, Stephen D.] Cornell Univ, Ithaca, NY 14853 USA. [Beaudette, Dylan E.] USDA, NRCS, Washington, DC USA. [Irons, James R.; O'Neill, Peggy E.] NASA, Goddard Space Flight Ctr, Greenbelt, MD USA. [Libohova, Zamir; Schoeneberger, Philip J.; Wysocki, Douglas A.] USDA, NRCS Natl Soil Survey Ctr, Washington, DC USA. [Owens, Philip R.] Purdue Univ, W Lafayette, IN 47907 USA. [West, Larry T.] Formerly USDA, NRCS Natl Soil Survey Ctr, Washington, DC USA. RP DeGloria, SD (reprint author), Cornell Univ, Ithaca, NY 14853 USA. EM sdd4@cornell.edu; debeaudette@ucdavis.edu; james.r.irons@nasa.gov; zamir.libohova@lin.usda.gov; peggy.e.oneill@nasa.gov; prowens@purdue.edu; Philip.Schoeneberger@lin.usda.gov; larrywestar@gmail.com; doug.wysocki@lin.usda.gov RI DeGloria, Stephen/B-8513-2015 OI DeGloria, Stephen/0000-0002-3343-6607 NR 26 TC 0 Z9 0 U1 1 U2 16 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD APR PY 2014 VL 80 IS 4 BP 289 EP 294 PG 6 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA AE3HK UT WOS:000333867400002 ER PT J AU Libohova, Z Doolittle, J Sims, R Villars, T West, LT AF Libohova, Zamir Doolittle, James Sims, Reed Villars, Thomas West, Larry T. TI Mapping the Subaqueous Soils of Lake Champlain's Missisquoi Bay Using Ground-Penetrating Radar, Digital Soil Mapping and Field Measurements SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID GPR SURVEY; SEDIMENTS; ESTUARY; MAINE; RIVER; PONDS AB Eutrophication associated with high concentrations of phosphorus in Missisquoi Bay has raised concerns about its influence on submersed aquatic vegetation. Subaqueous soils could play an important role in nutrient cycling, algae blooms, the spread of invasive aquatic vegetation, and water quality. The objectives of this study were to (a) characterize some of the morphological, physical and chemical properties of subaqueous soils in Missisquoi Bay based on ground-penetrating radar and soil analysis; and (b) assess relationships among the subaqueous soil landscapes, radar facies, and submersed aquatic vegetation. Coarse Stratified Sediments and Lacustrine Silt radar fades associated with Delta/Nearshore and Lakebed/Bay Bottom subaqueous soil-landscape units covered 90 percent of the study area. The Lacustrine Silt radar facies occurred in relatively deep, low-energy, depositional areas and had the highest P concentrations. The majority of the submersed aquatic vegetation was associated with Delta/Nearshore and Fringing Peatland subaqueous soil landscapes under shallow water. C1 [Libohova, Zamir] USDA NRCS Natl Soil Survey Ctr, Lincoln, NE 68508 USA. [Doolittle, James] USDA NRCS Natl Soil Survey Ctr, Newtown Sq, PA 19073 USA. [Sims, Reed] USDA NRCS, Colchester, VT 05446 USA. [Villars, Thomas] USDA NRCS, White River Jct, VT 05446 USA. RP Libohova, Z (reprint author), USDA NRCS Natl Soil Survey Ctr, 100 Centennial Mall North,Fed Bldg,Room 152, Lincoln, NE 68508 USA. EM zamir.libohova@lin.usda.gov RI DeGloria, Stephen/B-8513-2015 OI DeGloria, Stephen/0000-0002-3343-6607 NR 54 TC 1 Z9 1 U1 2 U2 17 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD APR PY 2014 VL 80 IS 4 BP 323 EP 332 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA AE3HK UT WOS:000333867400007 ER PT J AU Nauman, TW Thompson, JA Rasmussen, C AF Nauman, Travis W. Thompson, James A. Rasmussen, Craig TI Semi-Automated Disaggregation of a Conventional Soil Map Using Knowledge Driven Data Mining and Random Forests in the Sonoran Desert, USA SO PHOTOGRAMMETRIC ENGINEERING AND REMOTE SENSING LA English DT Article ID CLASSIFICATION TREE ANALYSIS; DIGITAL ELEVATION MODELS; FUZZY-LOGIC; VEGETATION PATTERNS; REGIONAL-SCALE; LANDSCAPE; INFORMATION; SYSTEM; EXTRAPOLATION; ATTRIBUTES AB Conventional soil maps (CSM) have provided baseline soil information for land use planning for over 100 years. Although CSM have been widely used, they are not suitable to meet growing demands for high resolution soil information at field scales. We present a repeatable method to disaggregate CSM data into similar to 30-meter resolution rasterized soil class maps that include continuous representation of probabilistic map uncertainty. Methods include training set creation for original CSM component soil classes from soil-landscape descriptions within the original survey database. Training sets are used to build a random forest predictive model utilizing environmental covariate maps derived from ASTER satellite imagery and the USGS National Elevation Dataset. Results showed agreement at 70 percent of independent field validation sites and equivalent accuracy between original CSM map units and the disaggregated map. Uncertainty of predictions was mapped by relating prediction frequencies of the random forest model and success rates at validation sites. C1 [Nauman, Travis W.] Nat Resources Conservat Serv, USDA, Natl Soil Survey Ctr, Geospatial Res Unit, Morgantown, WV 26506 USA. [Nauman, Travis W.; Thompson, James A.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Rasmussen, Craig] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. RP Nauman, TW (reprint author), Nat Resources Conservat Serv, USDA, Natl Soil Survey Ctr, Geospatial Res Unit, 1090 Agr Sci Bldg,POB 6108, Morgantown, WV 26506 USA. EM tnauman@mix.wvu.edu RI DeGloria, Stephen/B-8513-2015 OI DeGloria, Stephen/0000-0002-3343-6607 FU USDA-NRCS [68-9457-8-466, 68-7482-12-535, 68-3A75-4-100, 68-7482-11-527, 68-7482-9-511]; Arizona Agricultural Experiment Station [ARZT-1367190-H21-155] FX We wish to acknowledge Sue Rutman and the rest of the ORPI staff for their insight and help, as well as Chris Bertrand and Corrie Hannah for dedicated assistance in the field. Portions of this research were supported by USDA-NRCS Cooperative Agreements No. 68-9457-8-466 and No. 68-7482-12-535 with Dr. Craig Rasmussen, as well as Arizona Agricultural Experiment Station ARZT-1367190-H21-155. Funding supporting Travis Nauman at the University of Arizona came through USDA-NRCS Cooperative Agreement No. 68-3A75-4-100. Additional funding was provided by USDA-NRCS Cooperative Agreements No. 68-7482-11-527 and No. 68-7482-9-511 with Dr. James Thompson. Scientific contribution No. 3193 from the West Virginia Agricultural and Forestry Experiment Station, Morgantown, WV. NR 89 TC 4 Z9 4 U1 2 U2 26 PU AMER SOC PHOTOGRAMMETRY PI BETHESDA PA 5410 GROSVENOR LANE SUITE 210, BETHESDA, MD 20814-2160 USA SN 0099-1112 J9 PHOTOGRAMM ENG REM S JI Photogramm. Eng. Remote Sens. PD APR PY 2014 VL 80 IS 4 BP 353 EP 366 PG 14 WC Geography, Physical; Geosciences, Multidisciplinary; Remote Sensing; Imaging Science & Photographic Technology SC Physical Geography; Geology; Remote Sensing; Imaging Science & Photographic Technology GA AE3HK UT WOS:000333867400010 ER PT J AU Zhang, Y Long, RJ Warzecha, CM Coverdale, JA Latham, EA Hume, ME Callaway, TR O'Neil, MR Beier, RC Anderson, RC Nisbet, DJ AF Zhang, Ying Long, Ruijun Warzecha, Christine M. Coverdale, Josie A. Latham, Elizabeth A. Hume, Michael E. Callaway, Todd R. O'Neil, Mathew R. Beier, Ross C. Anderson, Robin C. Nisbet, David J. TI Characterization of bovine ruminal and equine cecal microbial populations enriched for enhanced nitro-toxin metabolizing activity SO ANAEROBE LA English DT Article DE Hydrogen; Carbon dioxide; Formate; Nitropropionic acid metabolism; Detoxification ID AMERICAN ASTRAGALUS FABACEAE; 3-NITROPROPIONIC ACID; METHANE PRODUCTION; SP-NOV; BACTERIUM; RUMEN; NITROCOMPOUNDS; NITROPROPANOL; HYDROGEN; CATTLE AB The nitrotoxins 3-nitro-1-propionic acid (NPA) and 3-nitro-1-propanol (NPOH) are produced by a wide variety of leguminous plants, including over 150 different species and varieties of Astragalus potentially grazed by livestock. These toxins are known to be detoxified by at least one ruminal bacterium but detoxification by bacteria from other gut habitats is not known. In the present study, mixed populations of bovine ruminal and equine cecal microbes were enriched for NPA-metabolizing bacteria via consecutive 24-72 h culture in a basal minimal rumen fluid-based medium supplemented with 4.2 mM NPA and H-2 as the energy source. Rates of NPA metabolism by the respective populations increased from 58.4 +/- 4.8 and 8.6 +/- 11.6 nmol NPA/mL per h during initial culture to 88.9 +/- 30.6 and 50.2 +/- 30.9 nmol NPA/mL per h following enrichment. Results from 3-tube most probable number tests indicated that numbers of NPA-degrading microbes increased 2.1 and 1.8 log(10) units during enrichment from numbers measured pre-enrichment (3.9 x 10(3) and 4.3 x 10(1) cells/mL for ruminal and equine cecal populations, respectively). Hydrogen, formate, and to a lesser extent, in-lactic acid, served as electron donors to the enriched populations and CO2 or formate were needed to maintain high rates of NPA-metabolism. The NPA-enriched populations were able to metabolize nitrate which, being a preferred electron acceptor, was antagonistic to NPA metabolism. Supplemental NPA was inhibitory to methanogenesis. Fermentation balance estimates indicated that only 47.6% of carbon available in potential substrates was recovered in headspace CO2, volatile fatty acids or unmetabolized NPA after 72 h incubation of NPA-enriched populations that had metabolized 98% of 8.4 mM added NPA. Overall, these results reveal low level carriage of NPA-metabolizing, CO2 or formate-requiring bacterial populations in the equine cecum yet support the concept that Denitrobacterium detoxificans-like organisms may well be the functional agents of NPA and NPOH detoxification in the populations studied here. Published by Elsevier Ltd. C1 [Zhang, Ying; Long, Ruijun] Lanzhou Univ, Coll Pastoral Agr Sci & Technol, Tibetan Rangeland & Yak Res Inst, Lanzhou 730020, Peoples R China. [Zhang, Ying; Long, Ruijun] Lanzhou Univ, Int Ctr Tibetan Plateau Ecosyst Management, Lanzhou 730020, Peoples R China. [Zhang, Ying; Hume, Michael E.; Callaway, Todd R.; Beier, Ross C.; Anderson, Robin C.; Nisbet, David J.] ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, College Stn, TX 77845 USA. [Warzecha, Christine M.; Coverdale, Josie A.; Latham, Elizabeth A.] Texas A&M Univ, Dept Anim Sci, College Stn, TX 77843 USA. [O'Neil, Mathew R.] Iowa State Univ, Dept Anim Sci, Nutr Physiol Grp, Ames, IA 50011 USA. RP Anderson, RC (reprint author), ARS, USDA, Southern Plains Agr Res Ctr, Food & Feed Safety Res Unit, 2881 F&B Rd, College Stn, TX 77845 USA. EM robin.anderson@ars.usda.gov OI Beier, Ross/0000-0002-3445-1008 FU Lanzhou University; Specialized Research Fund for the Doctoral Program of Higher Education [20120211110030]; Chinese Scholarship Council FX This research was supported in part by the Scholarship Award for Excellent Doctoral Student Granted by Lanzhou University and by the Specialized Research Fund for the Doctoral Program of Higher Education (20120211110030). The financial support of overseas study to author from Chinese Scholarship Council is greatly appreciated. The expert technical assistance of Jackie Kotzur and Syrita Murray is greatly appreciated. NR 42 TC 3 Z9 3 U1 0 U2 16 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1075-9964 EI 1095-8274 J9 ANAEROBE JI Anaerobe PD APR PY 2014 VL 26 BP 7 EP 13 DI 10.1016/j.anaerobe.2013.12.001 PG 7 WC Microbiology SC Microbiology GA AD8IW UT WOS:000333510700002 PM 24374155 ER PT J AU McMurphy, CP Sexten, AJ Mourer, GL Sharman, ED Trojan, SJ Rincker, MJ Coblentz, WK Lalman, DL AF McMurphy, C. P. Sexten, A. J. Mourer, G. L. Sharman, E. D. Trojan, S. J. Rincker, M. J. Coblentz, W. K. Lalman, D. L. TI Effects of including saponins (Micro-Aid (R)) on intake, rumen fermentation and digestibility in steers fed low-quality prairie hay SO ANIMAL FEED SCIENCE AND TECHNOLOGY LA English DT Article DE Micro-Aid (R); Saponins; Beef cattle; Low-quality hay; Protein supplementation; Digestibility ID YUCCA-SCHIDIGERA EXTRACT; COTTONSEED MEAL SUPPLEMENTATION; NEUTRAL DETERGENT FIBER; LACTATING DAIRY-COWS; BEEF-CATTLE; IN-VITRO; RUMINAL FERMENTATION; AMMONIA CONCENTRATIONS; NITROGEN-METABOLISM; PROTEIN-DEGRADATION AB Sixteen ruminally cannulated crossbred steers (529 +/- 45 kg initial body weight, BW) were used to evaluate in situ dry matter (DM), neutral detergent fiber (aNDF), and N degradation characteristics of low-quality prairie hay, blood urea-N (BUN) and rumen fermentation parameters in steers provided a protein supplement with or without Micro-Aid (R) (MA; plant derived saponin). Steers were allowed ad libitum access to chopped prairie hay (49g crude protein (CP)/kg DM and 738g aNDF/kg DM) and randomly assigned to one of four treatments: (1) no supplement (C), (2) cottonseed meal and wheat middlings: 920g DM/d (PC; positive control), (3) MA added to PC to supply 1 g MA/d (MA1), and (4) MA added to PC to supply 2 g MA/d (MA2). Steers were individually supplemented 920 g DM once daily at 08:00 along with a vitamin and mineral mix to ensure requirements were met. Orthogonal contrasts were used to determine the effects of protein supplementation, addition of MA and level of MA inclusion. During in situ phase, forage samples were incubated for a 96 h period. Protein supplementation increased DM intake (DMI), particulate passage rate (Kp), and rumen digestibility of DM and NDF (P < 0.001), but there was no effect on rumen N degradability. The inclusion of MA did not impact DMI in either phase. Compared to PC, MA decreased K-p (27.8 and 22.7 g DM/kg/h, respectively; P = 0.02), resulting in an increase in rumen aNDF and DM digestibility (P < 0.001). However, there was no influence of MA on apparent total tract digestibility in the metabolism phase. Rumen protozoa concentrations were suppressed (P = 0.01) with MA inclusion while lactate concentrations and microbial crude protein (MCP) flow to the small intestine were increased (P = 0.05). There was no impact on BUN, rumen ammonia, pH, volatile fatty acid (VFA) concentrations or N balance for MA compared to PC diets. Supplementation improved N balance, MCP synthesis and increased total concentrations of VFA and independent acetate and propionate concentrations. In conclusion, including MA in protein supplements increased rumen DM and a NDF digestibility of forage, reduced protozoa concentrations and increased daily outflow of MCP. This is indicative of increased rumen fermentation rate and may ultimately impact animal performance via increased energy and amino acid supply to the small intestine. However, more research is needed to validate this potential impact on animal performance. (C) 2014 Elsevier B.V. All rights reserved. C1 [McMurphy, C. P.; Sexten, A. J.; Mourer, G. L.; Sharman, E. D.; Trojan, S. J.; Lalman, D. L.] Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA. [Rincker, M. J.] DPI Global, Porterville, CA 93257 USA. [Coblentz, W. K.] USDA ARS, US Dairy Forage Res Ctr, Marshfield, WI 54449 USA. RP Lalman, DL (reprint author), Oklahoma State Univ, Dept Anim Sci, Stillwater, OK 74078 USA. EM david.lalman@okstate.edu NR 61 TC 2 Z9 3 U1 2 U2 20 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-8401 EI 1873-2216 J9 ANIM FEED SCI TECH JI Anim. Feed Sci. Technol. PD APR PY 2014 VL 190 BP 47 EP 58 DI 10.1016/j.anifeedsci.2014.01.007 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA AE2DN UT WOS:000333783600005 ER PT J AU Zhang, L Yang, M Li, C Xu, Y Sun, J Lei, C Lan, X Zhang, C Chen, H AF Zhang, L. Yang, M. Li, C. Xu, Y. Sun, J. Lei, C. Lan, X. Zhang, C. Chen, H. TI Identification and genetic effect of a variable duplication in the promoter region of the cattle ADIPOQ gene SO ANIMAL GENETICS LA English DT Article DE Adiponectin; bovine; growth traits; mRNA expression; promoter activity; variation ID PLASMA ADIPONECTIN LEVELS; TANDEM REPEAT SEQUENCES; INSULIN SENSITIVITY; WAIST CIRCUMFERENCE; CARCASS TRAITS; GENOME-WIDE; POLYMORPHISMS; ASSOCIATION; GLUCOSE; WEIGHT AB In cattle, the ADIPOQ gene is located in the vicinity of the quantitative trait locus (QTL) affecting marbling, the ribeye muscle area and fat thickness on BTA1. In our study, a novel variable duplication (NW_003103812.1:g.9232067_9232133dup) in the bovine ADIPOQ promoter region was identified and genotyped in seven Chinese cattle breeds. Using a reporter assay, we demonstrated that g.9232067_9232133dup decreased the basal transcriptional activity of the ADIPOQ gene in the 3T3-L1 and C2C12 cells. Furthermore, g.9232067_9232133dup suppressed the mRNA expression of the gene in adipose and muscle tissues. An association analysis indicated that the incremental variable duplication was associated with body measurements. C1 [Zhang, L.; Yang, M.; Xu, Y.; Sun, J.; Lei, C.; Lan, X.; Chen, H.] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China. [Li, C.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Zhang, C.] Jiangsu Normal Univ, Inst Cellular & Mol Biol, Xuzhou 221116, Jiangsu, Peoples R China. RP Chen, H (reprint author), Northwest A&F Univ, Coll Anim Sci & Technol, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China. EM chenhong1212@263.net FU National 863 Program of China [2013AA102505]; National Natural Science Foundation of China [30972080]; Agricultural Science and Technology Innovation Projects of Shaanxi Province [2012NKC01-13]; Program of the National Beef Cattle and Yak Industrial Technology System [CARS-38] FX This study was supported by the National 863 Program of China (Grant No. 2013AA102505), the National Natural Science Foundation of China (grant no. 30972080), the Agricultural Science and Technology Innovation Projects of Shaanxi Province (no. 2012NKC01-13) and Program of the National Beef Cattle and Yak Industrial Technology System (CARS-38). NR 36 TC 5 Z9 7 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD APR PY 2014 VL 45 IS 2 BP 171 EP 179 DI 10.1111/age.12112 PG 9 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AB9UA UT WOS:000332140000002 PM 24341634 ER PT J AU Porto-Neto, LR Lee, SH Sonstegard, TS Van Tassell, CP Lee, HK Gibson, JP Gondro, C AF Porto-Neto, L. R. Lee, S. H. Sonstegard, T. S. Van Tassell, C. P. Lee, H. K. Gibson, J. P. Gondro, C. TI Genome-wide detection of signatures of selection in Korean Hanwoo cattle SO ANIMAL GENETICS LA English DT Article DE bos indicus; bos taurus; selection; SNP; speciation ID HORMONE-SENSITIVE LIPASE; ARTIFICIAL SELECTION; POPULATION-STRUCTURE; GENETIC DIVERSITY; DAIRY-CATTLE; RFX COMPLEX; BOS-TAURUS; EXPRESSION; IDENTIFICATION; HOMOZYGOSITY AB The Korean Hanwoo cattle have been intensively selected for production traits, especially high intramuscular fat content. It is believed that ancient crossings between different breeds contributed to forming the Hanwoo, but little is known about the genomic differences and similarities between other cattle breeds and the Hanwoo. In this work, cattle breeds were grouped by origin into four types and used for comparisons: the Europeans (represented by six breeds), zebu (Nelore), African taurine (N'Dama) and Hanwoo. All animals had genotypes for around 680000 SNPs after quality control of genotypes. Average heterozygosity was lower in Nelore and N'Dama (0.22 and 0.21 respectively) than in Europeans (0.26-0.31, with Shorthorn as outlier at 0.24) and Hanwoo (0.29). Pairwise F-ST analyses demonstrated that Hanwoo are more related to European cattle than to Nelore, with N'Dama in an intermediate position. This finding was corroborated by principal components and unsupervised hierarchical clustering. Using genome-wide smoothed F-ST, 55 genomic regions potentially under positive selection in Hanwoo were identified. Among these, 29 were regions also detected in previous studies. Twenty-four regions were exclusive to Hanwoo, and a number of other regions were shared with one or two of the other groups. These regions overlap a number of genes that are related to immune, reproduction and fatty acid metabolism pathways. Further analyses are needed to better characterize the ancestry of the Hanwoo cattle and to define the genes responsible to the identified selection peaks. C1 [Porto-Neto, L. R.; Gibson, J. P.; Gondro, C.] Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia. [Porto-Neto, L. R.] Univ Queensland, Sch Vet Sci, Gatton, Qld 4345, Australia. [Lee, S. H.] RDA, Natl Inst Anim Sci, Hanwoo Expt Stn, Pyeongchang 232952, South Korea. [Sonstegard, T. S.; Van Tassell, C. P.] ARS, USDA, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Lee, H. K.] Hankyong Natl Univ, Dept Biotechnol, Anseong 456749, South Korea. RP Gondro, C (reprint author), Univ New England, Sch Environm & Rural Sci, Armidale, NSW 2351, Australia. EM laercio.portoneto@csiro.au; cgondro2@une.edu.au RI Porto-Neto, Laercio/D-2594-2012; gondro, cedric/B-4362-2010; OI Porto-Neto, Laercio/0000-0002-3536-8265; gondro, cedric/0000-0003-0666-656X; Van Tassell, Curtis/0000-0002-8416-2087; Gibson, John/0000-0003-0371-2401 FU Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008196] FX This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ008196), Rural Development Administration, Republic of Korea. The authors wish to thank Dr. Richard Spelman and the Livestock Improvement Corporation (LIC), New Zealand, and the Bovine Hapmap Consortium for sharing some genotypes used in this study. The authors also would like to thank the thoughtful comments of the anonymous reviewers that improved the quality of the manuscript. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 63 TC 9 Z9 9 U1 1 U2 10 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD APR PY 2014 VL 45 IS 2 BP 180 EP 190 DI 10.1111/age.12119 PG 11 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AB9UA UT WOS:000332140000003 PM 24494817 ER PT J AU Casas, E Duan, Q Schneider, MJ Shackelford, SD Wheeler, TL Cundiff, LV Reecy, JM AF Casas, E. Duan, Q. Schneider, M. J. Shackelford, S. D. Wheeler, T. L. Cundiff, L. V. Reecy, J. M. TI Polymorphisms in calpastatin and mu-calpain genes are associated with beef iron content SO ANIMAL GENETICS LA English DT Article DE CAST; Cattle; genetic markers; tenderness ID PALATABILITY TRAITS; LONGISSIMUS MUSCLE; CATTLE AB The objective of this study was to assess the association of markers in the calpastatin and mu-calpain loci with iron in beef cattle muscle. The population consisted of 259 cross-bred steers from Beefmaster, Brangus, Bonsmara, Romosinuano, Hereford and Angus sires. Total iron and heme iron concentrations were measured. Markers in the calpastatin (referred to as CAST) and mu-calpain (referred to as CAPN4751) genes were used to assess their association with iron levels. The mean and standard error for iron and heme iron content in the population was 35.6 +/- 1.3g and 27.1 +/- 1.4g respectively. Significant associations (P<0.01) of markers were observed for both iron and heme iron content. For CAST, animals with the CC genotype had higher levels of iron and heme iron in longissimus dorsi muscle. For CAPN4751, individuals with the TT genotype had higher concentrations of iron and heme iron than did animals with the CC and CT genotypes. Genotypes known to be associated with tougher meat were associated with higher levels of iron concentration. C1 [Casas, E.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Duan, Q.; Schneider, M. J.; Reecy, J. M.] Iowa State Univ, Ames, IA 50011 USA. [Shackelford, S. D.; Wheeler, T. L.; Cundiff, L. V.] ARS, US Meat Anim Res Ctr, USDA, Clay Ctr, NE 68933 USA. RP Casas, E (reprint author), ARS, Natl Anim Dis Ctr, USDA, 1920 Dayton Ave, Ames, IA 50010 USA. EM eduardo.casas@ars.usda.gov NR 12 TC 3 Z9 4 U1 0 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD APR PY 2014 VL 45 IS 2 BP 283 EP 284 DI 10.1111/age.12108 PG 2 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AB9UA UT WOS:000332140000015 PM 24303986 ER PT J AU White, SN Mousel, MR Reynolds, JO Herrmann-Hoesing, LM Knowles, DP AF White, S. N. Mousel, M. R. Reynolds, J. O. Herrmann-Hoesing, L. M. Knowles, D. P. TI Deletion variant near ZNF389 is associated with control of ovine lentivirus in multiple sheep flocks SO ANIMAL GENETICS LA English DT Article DE host control; maedi-visna virus; marker-assisted selection; ovine lentivirus; ovine progressive pneumonia; susceptibility; ZNF165; ZNF192; ZNF389; ZSCAN16 ID SMALL RUMINANT LENTIVIRUSES; MAEDI-VISNA CONTROL; VIRUS; INFECTION; PRODUCTIVITY AB Ovine lentivirus (OvLV) is a macrophage-tropic lentivirus found in many countries that causes interstitial pneumonia, mastitis, arthritis and cachexia in sheep. There is no preventive vaccine and no cure, but breed differences suggest marker-assisted selective breeding might improve odds of infection and control of OvLV post-infection. Although variants in TMEM154 have consistent association with odds of infection, no variant in any gene has been associated with host control of OvLV post-infection in multiple animal sets. Proviral concentration is a live-animal diagnostic measure of OvLV control post-infection related to severity of OvLV-induced lesions. A recent genome-wide association study identified a region including four zinc finger genes associated with proviral concentration in one Rambouillet flock. To refine this region, we tested additional variants and identified a small insertion/deletion variant near ZNF389 that showed consistent association with proviral concentration in three animal sets (P<0.05). These animal sets contained Rambouillet, Polypay and crossbred sheep from multiple locations and management conditions. Strikingly, one flock had exceptionally high prevalence (>87%, including yearlings) and mean proviral concentration (>950copies/g), possibly due to needle sharing. The best estimate of proviral concentration by genotype, obtained from all 1310 OvLV-positive animals tested, showed insertion homozygotes had less than half the proviral concentration of other genotypes (P<0.0001). Future work will test additional breeds, management conditions and viral subtypes, and identify functional properties of the haplotype this deletion variant tracks. To our knowledge, this is the first genetic variant consistently associated with host control of OvLV post-infection in multiple sheep flocks. C1 [White, S. N.; Reynolds, J. O.; Knowles, D. P.] ARS, Anim Dis Res Unit, USDA, Pullman, WA 99164 USA. [White, S. N.; Herrmann-Hoesing, L. M.; Knowles, D. P.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Mousel, M. R.] US Sheep Expt Stn, Dubois, ID 83423 USA. RP White, SN (reprint author), ARS, Anim Dis Res Unit, USDA, POB 646630, Pullman, WA 99164 USA. EM swhite@vetmed.wsu.edu OI White, Stephen N./0000-0001-9255-6054; Mousel, Michelle/0000-0003-1367-7005 FU USDA-ARS [5348-32000-031-00D] FX Thanks to Caylee Birge, Codie Durfee, Nic Durfee, Ralph Horn, James Allison, Natalie Pierce, Mark Williams and the farm crew of USSES for technical assistance. The authors thank James Kijas and CSIRO for providing reference guided assemblies of 75 sheep genomes (as BAM files) on behalf of the International Sheep Genomics Consortium under the Toronto guidelines for data users. This work was supported by USDA-ARS Grant 5348-32000-031-00D. NR 24 TC 3 Z9 4 U1 0 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD APR PY 2014 VL 45 IS 2 BP 297 EP 300 DI 10.1111/age.12107 PG 4 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AB9UA UT WOS:000332140000019 PM 24303974 ER PT J AU Garcia-Chevesich, PA Alvarado, S Neary, DG Valdes, R Valdes, J Aguirre, JJ Mena, M Pizarro, R Jofre, P Vera, M Olivares, C AF Garcia-Chevesich, Pablo A. Alvarado, Sergio Neary, Daniel G. Valdes, Rodrigo Valdes, Juan Jose Aguirre, Juan Mena, Marcelo Pizarro, Roberto Jofre, Paola Vera, Mauricio Olivares, Claudio TI Respiratory disease and particulate air pollution in Santiago Chile: Contribution of erosion particles from fine sediments SO ENVIRONMENTAL POLLUTION LA English DT Editorial Material DE PM10; Air pollution; Santiago; Erosion; Sedimentation ID PM2.5; PM10 AB Air pollution in Santiago is a serious problem every winter, causing thousands of cases of breathing problems within the population. With more than 6 million people and almost two million vehicles, this large city receives rainfall only during winters. Depending on the frequency of storms, statistics show that every time it rains, air quality improves for a couple of days, followed by extreme levels of air pollution. Current regulations focus mostly on PM10 and PM2.5, due to its strong influence on respiratory diseases. Though more than 50% of the ambient PM10s in Santiago is represented by soil particles, most of the efforts have been focused on the remaining 50%, i.e. particulate material originating from fossil and wood fuel combustion, among others. This document emphasizes the need for the creation of erosion/sediment control regulations in Chile, to decrease respiratory diseases on Chilean polluted cities. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Garcia-Chevesich, Pablo A.; Jose Aguirre, Juan; Jofre, Paola] Inst Forestal, Santiago, Chile. [Garcia-Chevesich, Pablo A.; Valdes, Rodrigo; Valdes, Juan] Univ Arizona, Dept Hydrol & Water Resources, Tucson, AZ 85721 USA. [Alvarado, Sergio] Univ Chile, Fac Med, Div Epidemiol, Escuela Salud Publ, Santiago, Chile. [Mena, Marcelo] Univ Andres Bello, Santiago, Chile. [Valdes, Rodrigo; Pizarro, Roberto; Vera, Mauricio; Olivares, Claudio] Univ Talca, Fac Ingn Forestal, Talca, Chile. [Alvarado, Sergio] Univ Tarapaca, Fac Ciencias Salud, Arica, Chile. [Neary, Daniel G.] USDA Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ USA. RP Pizarro, R (reprint author), Inst Forestal, Santiago, Chile. EM pablogarciach@gmail.com; rpizarro@utalca.cl RI Mena-Carrasco, Marcelo/L-9730-2016 NR 18 TC 2 Z9 3 U1 7 U2 44 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0269-7491 EI 1873-6424 J9 ENVIRON POLLUT JI Environ. Pollut. PD APR PY 2014 VL 187 BP 202 EP 205 DI 10.1016/j.envpol.2013.12.028 PG 4 WC Environmental Sciences SC Environmental Sciences & Ecology GA AD8BN UT WOS:000333491600025 PM 24485904 ER PT J AU Wu, L Munoz-Carpena, R Gao, B Yang, W Pachepsky, YA AF Wu, Lei Munoz-Carpena, Rafael Gao, Bin Yang, Wen Pachepsky, Yakov A. TI Colloid Filtration in Surface Dense Vegetation: Experimental Results and Theoretical Predictions SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID CRYPTOSPORIDIUM-PARVUM OOCYSTS; SATURATED POROUS-MEDIA; NATURAL ORGANIC-MATTER; LAMINAR OVERLAND-FLOW; FILTER STRIPS; ATTACHMENT EFFICIENCY; CYLINDRICAL COLLECTOR; ESCHERICHIA-COLI; IONIC-STRENGTH; PARTICLE-SIZE AB Understanding colloid and colloid-facilitated contaminant transport in overland flow through dense vegetation is important to protect water quality in the environment, especially for water bodies receiving agricultural and urban runoff. In previous studies, a single-stem efficiency theory for rigid and clean stem systems was developed to predict colloid filtration by plant stems of vegetation in laminar overland flow. Hence, in order to improve the accuracy of the single-stem efficiency theory to real dense vegetation system, we incorporated the effect of natural organic matter (NOM) on the filtration of colloids by stems. Laboratory dense vegetation flow chamber experiments and model simulations were used to determine the kinetic deposition (filtration) rate of colloids under various conditions. The results show that, in addition to flow hydrodynamics and solution chemistry, steric repulsion afforded by NOM layer on the plants stem surface also plays a significant role in controlling colloid deposition on vegetation in overland flow. For the first time, a refined single-stem efficiency theory with considerations of the NOM effect is developed that describes the experimental data with good accuracy. This theory can be used to not only help construct and refine mathematical models of colloid transport in real vegetation systems in overland flow, but also inform the development of theories of colloid deposition on NOM-coated surfaces in natural, engineered, and biomedical systems. C1 [Wu, Lei; Munoz-Carpena, Rafael; Gao, Bin] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Yang, Wen] Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA. [Pachepsky, Yakov A.] USDA ARS, Environm Microbial Safety Lab, Beltsville, MD 20705 USA. RP Munoz-Carpena, R (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM carpena@ufl.edu; bg55@ufl.edu RI Wu, Lei/B-7129-2015; Gao, Bin/B-8027-2010; OI Gao, Bin/0000-0003-3769-0191; Pachepsky, Yakov/0000-0003-0232-6090; Munoz-Carpena, Rafael/0000-0003-2838-1514 FU NSF [CHE-1213333]; UF Research Professorship; NIFA-SAES Multistate Project [S-1042]; NSF-REU FX L.W. and B.G. acknowledge partial financial support of this work by the NSF through Grant CHE-1213333. R.M.C. acknowledges support from the UF Research Professorship, UF Water Institute Faculty Fellow, and NIFA-SAES Multistate Project S-1042. W.Y. acknowledges support from an NSF-REU grant for the summer internship at the University of Florida. NR 47 TC 7 Z9 8 U1 9 U2 35 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD APR 1 PY 2014 VL 48 IS 7 BP 3883 EP 3890 DI 10.1021/es404603g PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AE2AQ UT WOS:000333776100035 PM 24597773 ER PT J AU Valenzano, S Lippolis, V Pascale, M De Marco, A Maragos, CM Suman, M Visconti, A AF Valenzano, Stefania Lippolis, Vincenzo Pascale, Michelangelo De Marco, Agostino Maragos, Chris M. Suman, Michele Visconti, Angelo TI Determination of Deoxynivalenol in Wheat Bran and Whole-Wheat Flour by Fluorescence Polarization Immunoassay SO FOOD ANALYTICAL METHODS LA English DT Article DE Deoxynivalenol; Fluorescence polarization immunoassay; Wheat bran; Whole-wheat flour ID MYCOTOXINS; FRACTIONS; COOKING; CEREALS; GRAINS; ASSAY AB A rapid and accurate fluorescence polarization (FP) immunoassay has been optimized for the determination of deoxynivalenol (DON) in wheat bran and whole-wheat flour. A preliminary treatment with activated charcoal was used to eliminate the strong matrix effect due to highly colored interfering compounds present in raw wheat bran extracts. In particular, matrix effect was removed by adding activated charcoal to the wheat bran extract (3.5 mg/mL) and mixing for 3 min of incubation time prior to the FP immunoassay analysis. No preliminary treatment was necessary for whole-wheat flour. Average recoveries from samples spiked with DON at levels of 500, 1,000, and 1,500 mu g/kg were 95 % for wheat bran and 94 % for whole-wheat flour, with relative standard deviation generally lower than 13 %. Limits of quantification of the optimized FP immunoassay were 120 mu g/kg for both matrices. The overall time of analysis was lower than 15 min for wheat bran and 10 min for whole-wheat flour. Good correlations (r > 0.971) were observed between DON contents obtained by both FP immunoassay and high-performance liquid chromatography with immunoaffinity cleanup for 37 and 23 samples of naturally contaminated wheat bran and whole-wheat flour, respectively. These results show that the FP immunoassay is suitable for high-throughput screening as well as for quantitative determination of DON in wheat bran and whole-wheat flour. C1 [Valenzano, Stefania; Lippolis, Vincenzo; Pascale, Michelangelo; De Marco, Agostino; Visconti, Angelo] Natl Res Council Italy CNR, Inst Sci Food Prod ISPA, I-70126 Bari, Italy. [Maragos, Chris M.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. [Suman, Michele] Barilla Food Res Labs, I-43122 Parma, Italy. RP Lippolis, V (reprint author), Natl Res Council Italy CNR, Inst Sci Food Prod ISPA, Via G Amendola 122-O, I-70126 Bari, Italy. EM vincenzo.lippolis@ispa.cnr.it OI Pascale, Michelangelo/0000-0002-3898-1030 FU Italian Ministry of Education, University and Research, MIUR [427/Ric., 02_00186_3417512] FX This work has been supported by the Italian Ministry of Education, University and Research, MIUR (PON 2007-2013, D.D. 427/Ric., 19 July 2012), project 02_00186_3417512 "S.I.Mi.S.A.". The authors would like to thank Giuseppe Panzarini (ISPA-CNR) and Sandro Zanardi (Barilla Food Research Labs) for their technical help in the HPLC analysis and preparation of samples. NR 35 TC 5 Z9 5 U1 4 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1936-9751 EI 1936-976X J9 FOOD ANAL METHOD JI Food Anal. Meth. PD APR PY 2014 VL 7 IS 4 BP 806 EP 813 DI 10.1007/s12161-013-9684-7 PG 8 WC Food Science & Technology SC Food Science & Technology GA AD8QU UT WOS:000333531500009 ER PT J AU Norman, KN Scott, HM Harvey, RB Norby, B Hume, ME AF Norman, Keri N. Scott, Harvey M. Harvey, Roger B. Norby, Bo Hume, Michael E. TI Comparison of Antimicrobial Susceptibility Among Clostridium difficile Isolated from an Integrated Human and Swine Population in Texas SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID REDUCED SUSCEPTIBILITY; PCR RIBOTYPE-027; FOOD ANIMALS; METRONIDAZOLE; RESISTANCE; CLINDAMYCIN; VANCOMYCIN; EMERGENCE; DIARRHEA; STRAINS AB Clostridium difficile can be a major problem in hospitals because the bacterium primarily affects individuals with an altered intestinal flora; this largely occurs through prolonged antibiotic use. Proposed sources of increased community-acquired infections are food animals and retail meats. The objective of this study was to compare the antimicrobial resistance patterns of C. difficile isolated from a closed, integrated population of humans and swine to increase understanding of the bacterium in these populations. Swine fecal samples were collected from a vertically flowing swine population consisting of farrowing, nursery, breeding, and grower/finisher production groups. Human wastewater samples were collected from swine worker and nonworker occupational group cohorts. Antimicrobial susceptibility testing was performed on 523 C. difficile strains from the population using the commercially available agar diffusion Epsilometer test (Etest((R))) for 11 different antimicrobials. All of the swine and human strains were susceptible to amoxicillin/clavulanic acid, piperacillin/tazobactam, and vancomycin. In addition, all of the human strains were susceptible to chloramphenicol. The majority of the human and swine strains were resistant to cefoxitin and ciprofloxacin. Statistically significant differences in antimicrobial susceptibility were found among the swine production groups for ciprofloxacin, tetracycline, amoxicillin/clavulanic acid, and clindamycin. No significant differences in antimicrobial susceptibility were found across human occupational group cohorts. We found that 8.3% of the swine strains and 13.3% of the human strains exhibited resistance to metronidazole. The finding of differences in susceptibility patterns between human and swine strains of C. difficile provides evidence that transmission between host species in this integrated population is unlikely. C1 [Norman, Keri N.; Norby, Bo] Texas A&M Univ, Coll Vet Med & Biomed Sci, Dept Vet Integrat Biosci, College Stn, TX USA. [Scott, Harvey M.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med Pathobiol, Manhattan, KS 66506 USA. [Harvey, Roger B.; Hume, Michael E.] ARS, Food & Feed Safety Res Unit, USDA, College Stn, TX USA. RP Norman, KN (reprint author), Texas A&M Int Univ, Dept Biol & Chem, 5201 Univ Blvd, Laredo, TX 78041 USA. EM keri.norman@tamiu.edu FU National Pork Board Grants [06-156, 08-188]; United States Department of Agriculture Cooperative State Research, Education, and Extension Service National Research Initiative Competitive Grants Program Section 32.1 [2003-35212-13298] FX This work was supported by the National Pork Board Grants (06-156 and 08-188) and United States Department of Agriculture Cooperative State Research, Education, and Extension Service National Research Initiative Competitive Grants Program Section 32.1 (Epidemiologic Approaches to Food Safety) (2003-35212-13298). NR 38 TC 2 Z9 2 U1 1 U2 6 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD APR 1 PY 2014 VL 11 IS 4 BP 257 EP 264 DI 10.1089/fpd.2013.1648 PG 8 WC Food Science & Technology SC Food Science & Technology GA AD9RR UT WOS:000333602700001 PM 24320797 ER PT J AU Folster, JP Tolar, B Pecic, G Sheehan, D Rickert, R Hise, K Zhao, SH Fedorka-Cray, PJ McDermott, P Whichard, JM AF Folster, Jason P. Tolar, Beth Pecic, Gary Sheehan, Deborah Rickert, Regan Hise, Kelley Zhao, Shaohua Fedorka-Cray, Paula J. McDermott, Patrick Whichard, Jean M. TI Characterization of bla(CMY) Plasmids and Their Possible Role in Source Attribution of Salmonella enterica Serotype Typhimurium Infections SO FOODBORNE PATHOGENS AND DISEASE LA English DT Article ID FIELD GEL-ELECTROPHORESIS; ESCHERICHIA-COLI; UNITED-STATES; BETA-LACTAMASES; INCI1 PLASMIDS; INCA/C PLASMID; FOOD ANIMALS; SUSCEPTIBILITY; RESISTANCE; PATHOGENS AB Salmonella is an important cause of foodborne illness; however, identifying the source of these infections can be difficult. This is especially true for Salmonella serotype Typhimurium, which is found in diverse agricultural niches. Extended-spectrum cephalosporins (ESC) are one of the primary treatment choices for complicated Salmonella infections. In Salmonella, ESC resistance in the United States is mainly mediated by bla(CMY) genes carried on various plasmids. In this study, we examined whether the characterization of bla(CMY) plasmids, along with additional information, can help us identify potential sources of infection by Salmonella, and used serotype Typhimurium as a model. In the United States, monitoring of retail meat, food animals, and ill persons for antimicrobial-resistant Salmonella is conducted by the National Antimicrobial Resistance Monitoring System. In 2008, 70 isolates (70/581; 12.0%) (34 isolates from retail meat, 23 food animal, and 13 human) were resistant to ceftriaxone and amoxicillin/clavulanic acid. All were polymerase chain reaction (PCR)-positive for bla(CMY) and 59/70 (84.3%) of these genes were plasmid encoded. PCR-based replicon typing identified 42/59 (71.2%) IncI1-bla(CMY) plasmids and 17/59 (28.8%) IncA/C-bla(CMY) plasmids. Isolates from chickens or chicken products with bla(CMY) plasmids primarily had IncI1-bla(CMY) plasmids (37/40; 92.5%), while all isolates from cattle had IncA/C-bla(CMY) plasmids. Isolates from humans had either IncA/C- bla(CMY) (n=8/12; [66.7%]) or IncI1- bla(CMY) (n=4/12 [33.3%]) plasmids. All of the IncI1-bla(CMY) plasmids were ST12 or were closely related to ST12. Antimicrobial susceptibility patterns (AST) and pulsed-field gel electrophoresis (PFGE) patterns of the isolates were also compared and differences were identified between isolate sources. When the source of a Typhimurium outbreak or sporadic illness is unknown, characterizing the outbreak isolate's bla(CMY) plasmids, AST, and PFGE patterns may help identify it. C1 [Folster, Jason P.; Tolar, Beth; Pecic, Gary; Sheehan, Deborah; Rickert, Regan; Hise, Kelley; Whichard, Jean M.] Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30329 USA. [Pecic, Gary] IHRC, Atlanta, GA USA. [Zhao, Shaohua; McDermott, Patrick] US FDA, Div Anim & Food Microbiol, Res Off, Ctr Vet Med, Laurel, MD USA. [Fedorka-Cray, Paula J.] ARS, Bacterial Epidemiol & Antimicrobial Resistance Re, USDA, Athens, GA USA. RP Folster, JP (reprint author), Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, 1600 Clifton Rd NE,MS G29, Atlanta, GA 30329 USA. EM gux8@cdc.gov FU CDC; USDA; FDA Center for Veterinary Medicine FX We thank the NARMS participating public health laboratories for submitting the isolates, Anne Whitney for DNA sequencing, and Alessandra Carattoli for the plasmid incompatibility typing control strains. This work was partially supported by an interagency agreement between CDC, USDA, and the FDA Center for Veterinary Medicine. NR 28 TC 4 Z9 4 U1 1 U2 4 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1535-3141 EI 1556-7125 J9 FOODBORNE PATHOG DIS JI Foodborne Pathog. Dis. PD APR 1 PY 2014 VL 11 IS 4 BP 301 EP 306 DI 10.1089/fpd.2013.1670 PG 6 WC Food Science & Technology SC Food Science & Technology GA AD9RR UT WOS:000333602700007 PM 24484290 ER PT J AU Haim, D White, EM Alig, RJ AF Haim, David White, Eric M. Alig, Ralph J. TI Permanence of agricultural afforestation for carbon sequestration under stylized carbon markets in the.US SO FOREST POLICY AND ECONOMICS LA English DT Article DE Permanence; Agricultural afforestation; Carbon markets; Economics ID CLIMATE-CHANGE; MITIGATION; TIMBER AB This paper examines the permanence of agricultural land afforestation under stylized carbon markets at the regional level in the US. Attention is focused on Southern and Midwest regions which historically have experienced a relatively large amount of land-use change between the agriculture and forest sectors. The Forest and Agriculture Sector Optimization Model-Greenhouse Gases model is used to examine responses between sectors as part of the regional afforestation policy analysis. Main findings suggest that most of afforested area in the Midwest regions remains unharvested by mid-21th century but a significant percentage of afforested area in the Southern regions shifts back to agricultural uses by this time. We also simulated a policy where carbon sequestration credits paid for afforestation are reduced 40% relative to other mitigation actions. A permanence value reduction for afforestation further promotes the harvesting of afforested stands in the Southern regions. Also, it has an impact not only on grassland pasture but also on high productive cropland. Results of this analysis are robust to lower permanence value reduction rates for higher carbon prices and can serve as upper bound of impacts for lower carbon prices. (C) 2014 Elsevier B.V. All rights reserved. C1 [Haim, David; White, Eric M.] Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. [Alig, Ralph J.] US Forest Serv, USDA, Pacific NW Res Stn, Whitley, KY USA. RP Haim, D (reprint author), Oregon State Univ, Dept Forest Engn Resources & Management, Corvallis, OR 97331 USA. EM david.haim@oregonstate.edu FU US Department of Agriculture, Economic Research Service FX This research was supported by the US Department of Agriculture, Economic Research Service. We appreciate the extremely helpful comments of the editor and two anonymous referees. NR 23 TC 2 Z9 2 U1 3 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 EI 1872-7050 J9 FOREST POLICY ECON JI Forest Policy Econ. PD APR PY 2014 VL 41 BP 12 EP 21 DI 10.1016/j.forpol.2013.12.008 PG 10 WC Forestry SC Forestry GA AE3CM UT WOS:000333854600002 ER PT J AU Adame, P Brandeis, TJ Uriarte, M AF Adame, Patricia Brandeis, Thomas J. Uriarte, Maria TI Diameter growth performance of tree functional groups in Puerto Rican secondary tropical forests SO FOREST SYSTEMS LA English DT Article DE Caribbean forests; growth model; tropical forest succession; Puerto Rico ID AREA INCREMENT MODEL; RAIN-FORESTS; ENVIRONMENTAL GRADIENTS; INDIVIDUAL TREES; AVAILABILITY; MOUNTAINS; CLIMATE AB Aim of study: Understanding the factors that control tree growth in successional stands is particularly important for quantifying the carbon sequestration potential and timber yield of secondary tropical forests. Understanding the factors that control tree growth in successional stands is particularly important for quantifying the carbon sequestration potential and timber yield of secondary tropical forests. Yet, the high species diversity of mixed tropical forests, including many uncommon species, hinders the development of species-specific diameter growth models. Area of study: In these analyses, we grouped 82 species from secondary forests distributed across 93 permanent plots on the island of Puerto Rico. Material and Methods: Species were classified according to regeneration strategy and adult height into six functional groups. This classification allowed us to develop a robust diameter growth model using growth data collected from 1980-1990. We used mixed linear model regression to analyze tree diameter growth as a function of individual tree characteristics, stand structure, functional group and site factors. Main results: The proportion of variance in diameter growth explained by the model was 15.1%, ranging from 7.9 to 21.7%. Diameter at breast height, stem density and functional group were the most important predictors of tree growth in Puerto Rican secondary forest. Site factors such as soil and topography failed to predict diameter growth. C1 [Adame, Patricia; Uriarte, Maria] Columbia Univ, Dept Ecol Evolut & Environm Biol, New York, NY 10027 USA. [Adame, Patricia] Dept Selvicultura & Gest Forestal CIFOR INIA, Madrid 28040, Spain. [Brandeis, Thomas J.] US Forest Serv, USDA, Southern Res Stn, Knoxville, TN 37919 USA. RP Adame, P (reprint author), Columbia Univ, Dept Ecol Evolut & Environm Biol, 1200 Amsterdam Ave, New York, NY 10027 USA. EM adame.patricia@inia.es NR 57 TC 1 Z9 1 U1 3 U2 33 PU INST NACIONAL INVESTIGACION TECHNOLOGIA AGRARIA ALIMENTARIA PI MADRID PA CTRA CORUNA KM 7 5, MADRID, 28040, SPAIN SN 2171-5068 EI 2171-9845 J9 FOREST SYST JI For. Syst. PD APR PY 2014 VL 23 IS 1 BP 52 EP 63 DI 10.5424/fs/2014231-03644 PG 12 WC Forestry SC Forestry GA AE2CG UT WOS:000333780300006 ER PT J AU Francisco, TI Orloski, KA Roberts, NJ AF Francisco, Tolani I. Orloski, Kathleen A. Roberts, Nancy J. TI Investigation of a Mycobacterium bovis outbreak in cattle at a Colorado dairy in 2010 SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Article ID TUBERCULOSIS AB Objective-To describe an epidemiological investigation of a bovine tuberculosis outbreak on a Colorado dairy operation. Procedures-A cull dairy cow infected with Mycobacterium bovis (index cow) was detected at a Texas abattoir during routine slaughter surveillance and subsequent diagnostic testing. This initiated an epidemiological investigation that was performed in accordance with USDA regulations. Results-The index cow was traced back to a Colorado dairy (index herd). Of the 908 cattle in the index herd, 101 (11.1%; 86 adult cattle > 2 years old and 15 immature cattle <= 2 years old) were infected with M bovis. Fourteen M bovis-infected cattle <= 2 years old were identified on 5 additional premises that had purchased cattle from the index herd directly or indirectly. All 115 affected cattle were infected with the same genetic type (spoligotype) of M bovis. A substantial proportion of cattle that left the index herd during the 5 years previous to the identification of the index cow were untraceable because of a lack of unique animal identification and inadequate records. Conclusions and Clinical Relevance-Results indicated that neonatal calves can have an important role in the transmission of M bovis. Also, this report highlights the exigent need for unique individual identification of livestock, including neonatal animals, so that thorough epidemiological investigations of reportable (zoonotic or foreign animal) diseases can be conducted when necessary. C1 [Francisco, Tolani I.] USDA, APHIS, Vet Serv, Colorado Area Off, Lakewood, CO 80125 USA. [Orloski, Kathleen A.] USDA, APHIS, Vet Serv, Ft Collins, CO 80526 USA. [Roberts, Nancy J.] USDA, APHIS, Vet Serv, Oklahoma Area Off, Oklahoma City, OK 73114 USA. RP Francisco, TI (reprint author), USDA, APHIS, Vet Serv, Colorado Area Off, 755 Parfet, Lakewood, CO 80125 USA. EM Tolani.I.Francisco@aphis.usda.gov NR 20 TC 5 Z9 5 U1 0 U2 0 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 EI 1943-569X J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD APR 1 PY 2014 VL 244 IS 7 BP 805 EP 812 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA AD8YP UT WOS:000333551900014 PM 24649991 ER PT J AU Butz, DE Casperson, SL Whigham, LD AF Buetz, Daniel E. Casperson, Shanon L. Whigham, Leah D. TI The emerging role of carbon isotope ratio determination in health research and medical diagnostics SO JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY LA English DT Review ID C-REACTIVE PROTEIN; GOAL-DIRECTED THERAPY; SEVERE SEPSIS; SEPTIC SHOCK; EMERGENCY-DEPARTMENT; BACTERIAL-INFECTION; UNITED-STATES; PROCALCITONIN; CHILDREN; EPIDEMIOLOGY AB Variations in the isotopic signature of carbon in biological samples (e.g. breath, blood and tissues) can be used to monitor shifts in whole body metabolism. As a conservative recorder of our diet, changes in the isotopic signature of carbon in biological samples provide an objective means to distinguish dietary patterns and the relationship with diseases. In addition, metabolic discrimination of carbon within the body can be informative regarding changes in the body's metabolic fuel usage during situations where shifts in the macronutrient oxidation ratio are expected. Therefore, changes in the isotopic signature over time have proven to be a tremendously powerful and sensitive means of detecting and measuring changes in steady-state systems. As such, this review focuses on how a naturally occurring ratio of stable isotopes of carbon (C-13/C-12) can be used as a biomarker for nutritional and metabolic status, altered macronutrient metabolism, and health and disease. C1 [Buetz, Daniel E.] Univ Wisconsin, Dept Anim Sci, Madison, WI 53706 USA. [Casperson, Shanon L.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Whigham, Leah D.] Paso Norte Inst Hlth Living, El Paso, TX 79968 USA. RP Butz, DE (reprint author), Univ Wisconsin, Dept Anim Sci, 1675 Observ Dr, Madison, WI 53706 USA. EM debutz@wisc.edu; ldwhigham@utep.edu OI Whigham, Leah/0000-0002-5376-8967; Casperson, Shanon/0000-0002-6954-9523 FU Isomark, LLC, (Madison, WI) FX We thank the Respiratory Therapy Department at the University of Wisconsin Hospital and Clinics for their participation in this project. We would like to thank Glen Leverson for consulting with us for statistical analysis of the data. Isomark, LLC, (Madison, WI) provided partial funding support for this study. NR 30 TC 1 Z9 1 U1 3 U2 17 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 0267-9477 EI 1364-5544 J9 J ANAL ATOM SPECTROM JI J. Anal. At. Spectrom. PD APR PY 2014 VL 29 IS 4 BP 594 EP 605 DI 10.1039/c3ja50327e PG 12 WC Chemistry, Analytical; Spectroscopy SC Chemistry; Spectroscopy GA AE0MJ UT WOS:000333660100001 ER PT J AU Dimick, SM Sallee, B Asztalos, BF Pritchard, PH Frohlich, J Schaefer, EJ AF Dimick, Susan M. Sallee, Brigitte Asztalos, Bela F. Pritchard, P. Haydn Frohlich, Jiri Schaefer, Ernst J. TI A kindred with fish eye disease, corneal opacities, marked high-density lipoprotein deficiency, and statin therapy SO JOURNAL OF CLINICAL LIPIDOLOGY LA English DT Article DE LCAT; Fish eye disease; Familial LCAT deficiency; ApoA-I; Alpha-1 HDL; Renal function; Atherosclerosis; Statin; HDL Map; Cholesterol esterification ID CHOLESTEROL ACYLTRANSFERASE ACTIVITY; LCAT DEFICIENCY; TANGIER DISEASE; LECITHIN; PLASMA; GENE AB A kindred affected with fish eye disease (FED) from Oklahoma is reported. Two probands with corneal pacification had mean levels of high-density lipoprotein (HDL) cholesterol (C), apolipoprotein (apo) A-I, and apoA-I in very large alpha-1 HDL particles that were 9%, 17%, and 5% of normal, whereas their parents and 1 sibling had values that were 61%, 77%, and 72% of normal. The probands had no detectable lipoprotein-X, and had mean low-density lipoprotein cholesterol (LDL-C) and triglycelide levels that were elevated. Their mean lecithin cholesterol acyltransferase (LCAT) activities, cholesterol esterification rates, and free cholesterol levels were 8%, 42%, and 258% of normal, whereas their parents and 1 sibling had values that were 55%, 49%, and 114% of normal. The defect was due to 1 common variant in the LCAT gene in exon 1:c101t causing a proline34leucine substitution and a novel mutation c1177t causing a threonine37methionine substitution, with the former variant being found in the father and 1 sibling, and the latter mutation being found in the mother, and both mutations being present in the 2 probands. FED is distinguished from familial LCAT deficiency (FLD) by the lack of anemia, splenomegaly, and renal insufficiency as well as normal or increased LDL-C. Both FLD and FED cases have marked HDL deficiency and corneal opacification, and FED cases may have premature coronary heart disease in contrast to FLD cases. Therapy, using presently available agents, in FED should be to optimize LDL-C levels, and 1 proband responded well to statin therapy. The investigational use of human recombinant LCAT as an enzyme source is ongoing. (C) 2014 National Lipid Association. All rights reserved. C1 [Dimick, Susan M.] Cent Oklahoma Early Detect Ctr, Lipidol & Cardiometabol Clin, Edmond, OK 73034 USA. [Dimick, Susan M.; Sallee, Brigitte] Univ Oklahoma, Coll Med, Oklahoma City, OK 73104 USA. [Asztalos, Bela F.; Schaefer, Ernst J.] Boston Heart Diagnost, Framingham, MA USA. [Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Human Nutr Res Ctr Aging, Lipid Metab Lab, Boston, MA 02111 USA. [Asztalos, Bela F.; Schaefer, Ernst J.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Pritchard, P. Haydn; Frohlich, Jiri] Univ British Columbia, St Pauls Hosp, Dept Pathol & Lab Med, Atherosclerosis Specialty Lab, Vancouver, BC V5Z 1M9, Canada. RP Dimick, SM (reprint author), Cent Oklahoma Early Detect Ctr, Lipidol & Cardiometabol Clin, 1227 East 9th St, Edmond, OK 73034 USA. EM susandimickmd@hotmail.com FU National Institutes of Health, Bethesda, MD [HL-60935, HL 74753, PO50HL083813]; US Department of Agriculture, Washington DC [53-3K06-5-10]; Boston Heart Laboratory, Framingham, MA FX This research was supported by research grants to the Lipid Metabolism Laboratory at Tufts University from the National Institutes of Health, Bethesda, MD (HL-60935, HL 74753 and PO50HL083813) and the US Department of Agriculture, Washington DC (contract 53-3K06-5-10). Drs. Polisecki, Asztalos, Ishida and Schaefer receive salary support from Boston Heart Laboratory, Framingham, MA. NR 19 TC 3 Z9 3 U1 0 U2 0 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1933-2874 EI 1876-4789 J9 J CLIN LIPIDOL JI J. Clin. Lipidol. PD APR PY 2014 VL 8 IS 2 BP 223 EP 230 DI 10.1016/j.jacl.2013.11.005 PG 8 WC Pharmacology & Pharmacy SC Pharmacology & Pharmacy GA AD8IH UT WOS:000333509200011 PM 24636183 ER PT J AU Schmidt, JW Bosilevac, JM Kalchayanand, N Wang, R Wheeler, TL Koohmaraie, M AF Schmidt, John W. Bosilevac, Joseph M. Kalchayanand, Norasak Wang, Rong Wheeler, Tommy L. Koohmaraie, Mohammad TI Immersion in Antimicrobial Solutions Reduces Salmonella enterica and Shiga Toxin-Producing Escherichia coli on Beef Cheek Meat SO JOURNAL OF FOOD PROTECTION LA English DT Article ID MULTIDRUG-RESISTANT SALMONELLA; SODIUM DODECYL-SULFATE; LACTIC-ACID; GROUND-BEEF; SUSCEPTIBLE SALMONELLA; PEROXYACETIC ACID; CARCASS SURFACES; AEROBIC-BACTERIA; LEVULINIC ACID; ORGANIC-ACIDS AB The objective of this study was to determine the effect of immersing beef cheek meat in antimicrobial solutions on the reduction of O157:117 Shiga toxin-producing Escherichia coli (STEC), non-O157:H7 STEC, and Salmonella enterica. Beef cheek meat was inoculated with O157:117 STEC, non-O157:H7 STEC, and S. enterica on both the adipose and muscle surfaces. The inoculated cheek meat was then immersed in one of seven antimicrobial solutions for 1, 2.5, or 5 min: (i) 1% Aftec 3000 (AFTEC), (ii) 2.5% Beefxide (BX), (iii) 300 ppm of hypobromous acid (HOBR), (iv) 2.5% lactic acid (LA2.5), (v) 5% lactic acid (LAS), (vi) 0.5% levulinic acid and 0.05% sodium dodecyl sulfate (LEV-SDS), or (vii) 220 ppm of peroxyacetic acid (POA). Inoculated cheek meat was also immersed in 80 degrees C tap water (HW) for 10 s. In general, increasing immersion duration in antimicrobial solutions did not significantly (P >= 0.05) increase effectiveness. Immersion in HW for 10 s was the most effective intervention, reducing STEC and S. enterica by 2.2 to 2.3 log CFU/cm(2) on the adipose surface and by 1.7 to 1.8 log CFU/cm(2) on the muscle surface. Immersion for 1 min in AFTEC, BX, LA2.5, LA5, or POA was also effective as an intervention, reducing STEC and S. enterica by 0.8 to 2.0 log CFU/cm(2) on the adipose surface and by 0.6 to 1.4 log CFU/cm(2) on the muscle surface. Immersion for 1 min in HOBR or LEV-SDS was not an effective intervention because STEC and S. enterica reductions ranged from 0.1 to 0.4 log CFU/cm(2), which were not significantly different (P >= 0.05) from the reductions obtained when cheek meat was immersed in room temperature tap water. We conclude that immersion of cheek meat in HW for 10 s and immersion for 1 min in AFTEC, BX, LA2.5, LA5, or POA effectively reduced levels of STEC and S. enterica. C1 [Schmidt, John W.; Bosilevac, Joseph M.; Kalchayanand, Norasak; Wang, Rong; Wheeler, Tommy L.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Koohmaraie, Mohammad] IEH Labs & Consulting Grp, Lake Forest Pk, WA 98155 USA. [Koohmaraie, Mohammad] King Saud Univ, Coll Food & Agr, Riyadh 11451, Saudi Arabia. RP Schmidt, JW (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM john.w.schmidt@ars.usda.gov OI Schmidt, John/0000-0003-0494-2436 FU Agriculture and Food Research Initiative from the U.S. Department of Agriculture [2011-68003-30012]; National Institute of Food and Agriculture, Food Safety: Food Processing Technologies to Destroy Food-borne Pathogens Program [A4131]; Distinguished Scientist Fellowship Program, King Saud University, Saudi Arabia FX The authors thank American Foods Group, LLC, for providing the cheek meat used in the project. We thank Advanced Food Technologies, Birko Corp., Ecolab, Enviro Tech Chemical Services Inc., and Purac for providing antimicrobial solutions. We thank Sydney Brodrick, Julie Dyer, Bruce Jasch, Roxanna Kipp, Lia Morales, Frank Reno, Gregory Smith, and Collin Whitsett for their technical support. We thank Jody Gallagher for secretarial assistance. This project was supported in part by Agriculture and Food Research Initiative grant no. 2011-68003-30012 from the U.S. Department of Agriculture, National Institute of Food and Agriculture, Food Safety: Food Processing Technologies to Destroy Food-borne Pathogens Program (A4131), and the Distinguished Scientist Fellowship Program, King Saud University, Saudi Arabia. NR 34 TC 5 Z9 5 U1 2 U2 13 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD APR PY 2014 VL 77 IS 4 BP 538 EP 548 DI 10.4315/0362-028X.JFP-13-300 PG 11 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AE1YL UT WOS:000333770300002 PM 24680064 ER PT J AU Zhou, B Luo, YG Nou, XW Millner, P AF Zhou, Bin Luo, Yaguang Nou, Xiangwu Millner, Patricia TI Development of an Algorithm for Feed-Forward Chlorine Dosing of Lettuce Wash Operations and Correlation of Chlorine Profile with Escherichia coli O157:H7 Inactivation SO JOURNAL OF FOOD PROTECTION LA English DT Article ID CUT PRODUCE WASH; CROSS-CONTAMINATION; BREAKPOINT CHLORINATION; WATER; REDUCTION; EFFICACY; AMMONIA; DISINFECTION; QUALITY AB The dynamic interactions of chlorine and organic matter during a simulated fresh-cut produce wash process and the consequences for Escherichia coli O157:H7 inactivation were investigated. An algorithm for a chlorine feed-forward dosing scheme to maintain a stable chlorine level was further developed and validated. Organic loads with chemical oxygen demand of 300 to 800 mg/liter were modeled using iceberg lettuce. Sodium hypochlorite (NaOCl) was added to the simulated wash solution incrementally. The solution pH, free and total chlorine, and oxidation-reduction potential were monitored, and chlorination breakpoint and chloramine humps determined. The results indicated that the E. coli O157:H7 inactivation curve mirrored that of the free chlorine during the chlorine replenishment process: a slight reduction in E. coli O157:H7 was observed as the combined chlorine hump was approached, while the E. coli O157:H7 cell populations declined sharply after chlorination passed the chlorine hump and decreased to below the detection limit (<0.75 most probable number per ml) after the chlorination breakpoint was reached. While the amounts of NaOCl required for reaching the chloramine humps and chlorination breakpoints depended on the organic loads, there was a linear correlation between NaOCl input and free chlorine in the wash solution once NaOCl dosing passed the chlorination breakpoint, regardless of organic load. The data obtained were further exploited to develop a NaOCl dosing algorithm for maintaining a stable chlorine concentration in the presence of an increasing organic load. The validation tests results indicated that free chlorine could be maintained at target levels using such an algorithm, while the pH and oxidation-reduction potential were also stably maintained using this system. C1 [Zhou, Bin; Luo, Yaguang; Nou, Xiangwu; Millner, Patricia] ARS, USDA, Beltsville Agr Res Ctr, Environm Microbiol & Food Safety Lab, Beltsville, MD 20705 USA. [Zhou, Bin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. RP Luo, YG (reprint author), ARS, USDA, Beltsville Agr Res Ctr, Environm Microbiol & Food Safety Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Yaguang.Luo@ars.usda.gov FU USDA-NIFA (Specialty Crop Initiative) [2010-01165] FX The authors wish to thank Dr. Arne Pearlstein (University of Illinois) and Mr. Jim Brennan (Taylor Farms, Salinas, CA) for critical reading of the manuscript. This research is supported by USDA-NIFA (Specialty Crop Initiative Award no. 2010-01165). NR 29 TC 6 Z9 6 U1 1 U2 13 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD APR PY 2014 VL 77 IS 4 BP 558 EP 566 DI 10.4315/0362-028X.JFP-13-352 PG 9 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AE1YL UT WOS:000333770300004 PM 24680066 ER PT J AU Davis, CD Bailey, RL Dwyer, JT Coates, PM AF Davis, Cindy D. Bailey, Regan L. Dwyer, Johanna T. Coates, Paul M. TI John Austin Milner, PhD (1947-2013) SO JOURNAL OF NUTRITION LA English DT Biographical-Item C1 [Davis, Cindy D.; Bailey, Regan L.; Dwyer, Johanna T.; Coates, Paul M.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. [Dwyer, Johanna T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Sch Med, Boston, MA 02111 USA. [Dwyer, Johanna T.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Davis, CD (reprint author), NIH, Off Dietary Supplements, Bldg 10, Bethesda, MD 20892 USA. EM davisci@mail.nih.gov OI Dwyer, Johanna/0000-0002-0783-1769 NR 1 TC 1 Z9 1 U1 0 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2014 VL 144 IS 4 BP 411 EP 413 DI 10.3945/jn.114.191064 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AE0MU UT WOS:000333661200001 PM 24500932 ER PT J AU Hansen, KE Nabak, AC Johnson, RE Marvdashti, S Keuler, NS Shafer, MM Abrams, SA AF Hansen, Karen E. Nabak, Andrea C. Johnson, Rachael Erin Marvdashti, Sheeva Keuler, Nicholas S. Shafer, Martin M. Abrams, Steven A. TI Isotope Concentrations from 24-h Urine and 3-h Serum Samples Can Be Used to Measure Intestinal Magnesium Absorption in Postmenopausal Women SO JOURNAL OF NUTRITION LA English DT Article ID BONE-MINERAL DENSITY; SECRETION; CHILDREN; CALCIUM; HUMANS AB Studies suggest a link between magnesium status and osteoporosis. One barrier to more conclusive research on the potential relation is measuring intestinal magnesium absorption (MgA), which requires the use of stable isotopes and a >= 6-d stool or 3-d urine collection. We evaluated alternative methods of measuring MgA. We administered 2 stable magnesium isotopes to 15 postmenopausal women (cohort 1) aged 62 +/- 8 y with a dietary magnesium intake of 345 +/- 72 mg/d. Participants fasted from 1200 h to 0700 h and then consumed breakfast with similar to 23 mg of oral Mg-26 and similar to 11 mg of i.v.Mg-25. We measured magnesium isotope concentrations in 72-h urine, spot urine (36, 48, 60, and 72 h), and spot serum (1, 3, and 5 h) samples collected after isotope dosing. We calculated MgA using the dose-corrected fraction of isotope concentrations from the 72-h urine collection. We validated new methods in 10 postmenopausal women (cohort 2) aged 59 +/- 5 y with a dietary magnesium intake of 325 +/- 122 mg/d. In cohort 1, MgA based on the 72-h urine collection was 0.28 +/- 0.08. The 72-h MgA correlated most highly with 0-24 h urine MgA value alone (rho = 0.95, P < 0.001) or the mean of the 0-24 h urine and the 3-h (p = 0.93, P < 0.001) or 5-h (rho = 0.96, P < 0.001) serum MgA values. In cohort 2, Bland-Altman bias was lowest (-0.003, P = 0.82) using means of the 0-24 h urine and 3-h serum MgA values. We conclude that means of 0-24 h urine and 3-h serum MgA provide a reasonable estimate of 72-h MgA. However, if researchers seek to identify small changes in MgA, we recommend a 3-d urine or extended stool collection. This trial was registered at clinicaltrials.gov as NCT01593501. C1 [Hansen, Karen E.; Johnson, Rachael Erin; Marvdashti, Sheeva] Univ Wisconsin, Dept Med, Sch Med & Publ Hlth, Madison, WI 53706 USA. [Nabak, Andrea C.] Univ Wisconsin, Dietet Program, Madison, WI USA. [Keuler, Nicholas S.] Univ Wisconsin, Coll Agr & Life Sci, Madison, WI 53706 USA. [Shafer, Martin M.] Univ Wisconsin, Madison, WI USA. [Shafer, Martin M.] Univ Wisconsin, Wisconsin State Lab Hyg, Madison, WI 53706 USA. [Abrams, Steven A.] Baylor Coll Med, Dept Pediat, Childrens Nutr Res Ctr, USDA ARS, Houston, TX 77030 USA. RP Hansen, KE (reprint author), Univ Wisconsin, Dept Med, Sch Med & Publ Hlth, Madison, WI 53706 USA. EM keh@medicine.wisc.edu OI Abrams, Steven/0000-0003-4972-9233 FU NIH; National Institute on Aging; Office of Dietary Supplements [R01A6028739] FX Supported by NIH, National Institute on Aging, and Office of Dietary Supplements, R01A6028739 and a supplemental grant NR 18 TC 1 Z9 1 U1 0 U2 4 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2014 VL 144 IS 4 BP 533 EP 537 DI 10.3945/jn.113.186767 PG 5 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AE0MU UT WOS:000333661200018 PM 24500940 ER PT J AU Poulose, SM Miller, MG Shukitt-Hale, B AF Poulose, Shibu M. Miller, Marshall G. Shukitt-Hale, Barbara TI Role of Walnuts in Maintaining Brain Health with Age SO JOURNAL OF NUTRITION LA English DT Article; Proceedings Paper CT Symposium on What Comes First - The Food or the Nutrient as a Satellite Session to the American-Society-for-Nutrition Scientific Sessions and Annual Meeting at Experimental Biology CY APR 19, 2013 CL Boston, MA SP Amer Soc Nutr ID CARDIOVASCULAR RISK-FACTORS; CEREBRAL METABOLIC-RATE; OXIDATIVE STRESS; NEURODEGENERATIVE DISEASES; ENDOTHELIAL FUNCTION; ALZHEIMERS-DISEASE; MEDITERRANEAN DIET; COGNITIVE FUNCTION; ANTIOXIDANT CAPACITY; HIPPOCAMPAL CELLS AB Because of the combination of population growth and population aging, increases in the incidence of chronic neurodegenerative disorders have become a societal concern, both in terms of decreased quality of life and increased financial burden. Clinical Manifestation of many of these disorders takes years, with the initiation of mild cognitive symptoms leading to behavioral problems, dementia and loss of motor functions, the need for assisted living, and eventual death. Lifestyle factors greatly affect the progression of cognitive decline, with high-risk behaviors including unhealthy diet, lack of exercise, smoking, and exposure to environmental toxins leading to enhanced oxidative stress and inflammation. Although there exists an urgent need to develop effective treatments for age-related cognitive decline and neurodegenerative disease, prevention strategies have been underdeveloped. Primary prevention in many of these neurodegenerative diseases could be achieved earlier in life by consuming a healthy diet, rich in antioxidant and anti-inflammatory phytochemicals, which offers one of the most effective and least expensive ways to address the crisis. English walnuts (Juglans regia L.) are rich in numerous phytochemicals, including high amounts of polyunsaturated fatty acids, and offer potential benefits to brain health. Polyphenolic compounds found in walnuts not only reduce the oxidant and inflammatory load on brain cells but also improve interneuronal signaling, increase neurogenesis, and enhance sequestration of insoluble toxic protein aggregates. Evidence for the beneficial effects of consuming a walnut-rich diet is reviewed in this article. C1 [Poulose, Shibu M.; Miller, Marshall G.; Shukitt-Hale, Barbara] Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov NR 61 TC 14 Z9 15 U1 6 U2 33 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD APR PY 2014 VL 144 IS 4 BP 561S EP 566S DI 10.3945/jn.113.184838 PG 6 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AE0MU UT WOS:000333661200023 PM 24500933 ER PT J AU Wang, S Su, R Nie, SF Sun, M Zhang, J Wu, DY Moustaid-Moussa, N AF Wang, Shu Su, Rui Nie, Shufang Sun, Ming Zhang, Jia Wu, Dayong Moustaid-Moussa, Naima TI Application of nanotechnology in improving bioavailability and bioactivity of diet-derived phytochemicals SO JOURNAL OF NUTRITIONAL BIOCHEMISTRY LA English DT Review DE Nanotechnology; Phytochemicals; Nanoparticles; Bioactivities; Biocompatible and biodegradable ID SOLID LIPID NANOPARTICLES; DRUG-DELIVERY SYSTEMS; CURCUMIN-LOADED PLGA; STERICALLY STABILIZED LIPOSOMES; OIL-IN-WATER; GREEN TEA; BIODEGRADABLE NANOPARTICLES; EPIGALLOCATECHIN GALLATE; INTESTINAL-ABSORPTION; TISSUE DISTRIBUTION AB Nanotechnology is an innovative approach that has potential applications in nutraceutical research. Phytochemicals have promising potential for maintaining and promoting health, as well as preventing and potentially treating some diseases. However, the generally low solubility, stability, bioavailability and target specificity, together with the side effects seen when used at high levels, have limited their application. Indeed, nanoparticles can increase solubility and stability of phytochemicals, enhance their absorption, protect them from premature degradation in the body and prolong their circulation time. Moreover, these nanoparticles exhibit high differential uptake efficiency in the target cells (or tissue) over normal cells (or tissue) through preventing them from prematurely interacting with the biological environment, enhanced permeation and retention effect in disease tissues and improving their cellular uptake, resulting in decreased toxicity, In this review, we outline the commonly used biocompatible and biodegradable nanoparticles including liposomes, emulsions, solid lipid nanoparticles, nanostructured lipid carriers, micelles and poly(lactic-co-glycolic acid) nanoparticles. We then summarize studies that have used these nanoparticles as carriers for epigallocatechin gallate, quercetin, resveratrol and curcumin administration to enhance their aqueous solubility, stability, bioavailability, target specificity and bioactivities. (C) 2014 Elsevier Inc. All rights reserved. C1 [Wang, Shu; Su, Rui; Nie, Shufang; Sun, Ming; Zhang, Jia; Moustaid-Moussa, Naima] Texas Tech Univ, Nutr Sci Program, Lubbock, TX 79409 USA. [Wu, Dayong] Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr Immunol Lab, Boston, MA 02111 USA. RP Wang, S (reprint author), Texas Tech Univ, Nutr Sci Program, Box 41240, Lubbock, TX 79409 USA. EM shu.wang@ttu.edu FU National Center for Complementary & Alternative Medicine [R15AT007013]; Texas Tech University, College of Human Sciences, Lubbock, TX, USA FX The project described was supported by Grant No. R15AT007013 from the National Center for Complementary & Alternative Medicine. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Center for Complementary & Alternative Medicine or the National Institutes of Health. Additional support was provided by Texas Tech University, College of Human Sciences, Lubbock, TX, USA. NR 162 TC 52 Z9 55 U1 8 U2 110 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0955-2863 EI 1873-4847 J9 J NUTR BIOCHEM JI J. Nutr. Biochem. PD APR PY 2014 VL 25 IS 4 BP 363 EP 376 DI 10.1016/j.jnutbio.2013.10.002 PG 14 WC Biochemistry & Molecular Biology; Nutrition & Dietetics SC Biochemistry & Molecular Biology; Nutrition & Dietetics GA AD8JU UT WOS:000333513100001 PM 24406273 ER PT J AU Cosentino, BJ Schooley, RL Bestelmeyer, BT Kelly, JF Coffman, JM AF Cosentino, Bradley J. Schooley, Robert L. Bestelmeyer, Brandon T. Kelly, Jeffrey F. Coffman, John M. TI Constraints and time lags for recovery of a keystone species (Dipodomys spectabilis) after landscape restoration SO LANDSCAPE ECOLOGY LA English DT Article DE Colonization; Connectivity; Conservation; Desertification; Dispersal; Ecosystem engineer; Grassland; Kangaroo rat; Shrub encroachment ID DESERT RODENT COMMUNITY; TAILED KANGAROO RATS; NEW-MEXICO; METAPOPULATION DYNAMICS; CONNECTIVITY MEASURES; SPATIAL-ORGANIZATION; CHIHUAHUAN DESERT; HABITAT SELECTION; GRASSLAND; ECOLOGY AB Habitat restoration is typically focused on reestablishing suitable conditions at a local scale, but landscape constraints may be important for keystone species with limited dispersal. We tested for time lags and examined the relative importance of local and landscape constraints on the response of the banner-tailed kangaroo rat (Dipodomys spectabilis) to restoration of Chihuahuan Desert grasslands in New Mexico, USA. Dipodomys spectabilis is a keystone species that creates habitat heterogeneity and modifies the structure of plant and animal communities. We selected 21 sites and compared density of D. spectabilis between areas treated with herbicide to control shrubs (treated areas) and paired untreated areas. We evaluated whether density of D. spectabilis depended on treatment age, local habitat quality (vegetation and soil structure), and landscape factors (treatment area and spatial connectivity). Density was greater at treated areas than at untreated areas due to a direct effect of reduced shrub cover. However, the response of D. spectabilis to restoration was lagged by a decade or more. Structural equation modeling indicated the time lag reflected a dispersal constraint as opposed to a temporal change in habitat quality. This inference was corroborated by a positive relationship between density at treated areas and connectivity to source populations. Our results indicate that density of D. spectabilis depended strongly on the spatial configuration of treated areas, which supports a landscape mosaic approach to restoration. If keystone species commonly exhibit limited dispersal ability, landscape constraints may be broadly important for shaping ecosystem structure and function after habitat restoration. C1 [Cosentino, Bradley J.] Hobart & William Smith Coll, Dept Biol, Geneva, NY 14456 USA. [Schooley, Robert L.; Coffman, John M.] Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USA. [Bestelmeyer, Brandon T.; Coffman, John M.] New Mexico State Univ, USDA ARS Jornada Expt Range, Las Cruces, NM 88003 USA. [Kelly, Jeffrey F.] Univ Oklahoma, Oklahoma Biol Survey, Norman, OK 73019 USA. [Kelly, Jeffrey F.] Univ Oklahoma, Dept Biol, Norman, OK 73019 USA. RP Cosentino, BJ (reprint author), Hobart & William Smith Coll, Dept Biol, Geneva, NY 14456 USA. EM cosentino@hws.edu RI Kelly, Jeffrey/B-2029-2015 OI Kelly, Jeffrey/0000-0002-8255-7990 FU USDA-AFRI Managed Ecosystems program; BLM FX This research was supported by a grant from the USDA-AFRI Managed Ecosystems program and by the BLM. We thank K. Sierzega for assistance in the field. L. Burkett and R. Lister were instrumental in providing logistical support. NR 60 TC 2 Z9 2 U1 11 U2 67 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD APR PY 2014 VL 29 IS 4 BP 665 EP 675 DI 10.1007/s10980-014-0003-5 PG 11 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA AD8RR UT WOS:000333533800009 ER PT J AU Huebner, CD Steinman, J Hutchinson, TF Ristau, TE Royo, AA AF Huebner, Cynthia D. Steinman, Jim Hutchinson, Todd F. Ristau, Todd E. Royo, Alejandro A. TI The distribution of a non-native (Rosa multiflora) and native (Kalmia latifolia) shrub in mature closed-canopy forests across soil fertility gradients SO PLANT AND SOIL LA English DT Article DE Invasive nonnative plant; Expansive native plant; Kalmia latifolia; Rosa multiflora; Soil fertility gradient ID NITROGEN MINERALIZATION; SOUTHERN APPALACHIANS; ELEVATION GRADIENT; DECIDUOUS FORESTS; NUTRIENT STATUS; UNITED-STATES; WEST-VIRGINIA; SHEEP LAUREL; OAK FORESTS; VEGETATION AB A soil fertility gradient, ranging from infertile to highly fertile soils, may define whether or not a plant will establish and spread at a site. We evaluated whether or not such a fertility gradient exists for Rosa multiflora Thunb., a nonnative invasive shrub, and Kalmia latifolia L., a native problem shrub, in closed-canopy forests of the eastern U.S. We sampled soil and vegetation at the regional scale, along four randomly located 1-km transects in 70+ year-old undisturbed forests in each of three national forests in Ohio, Pennsylvania and West Virginia. We also sampled soil, vegetation and leaf tissue at the local scale, from ten individual shrubs of each species in each national forest. Regional analyses showed a significant fertility gradient with Ohio being the most fertile and West Virginia the least. Soil fertility was associated with pH (most acidic in West Virginia and least acidic in Ohio) and elevation (highest in West Virginia and lowest in Ohio). At the local level, R. multiflora was associated with soil Ca:Al ratios greater than 0.5, and K. latifolia was associated with Ca:Al ratios less than 0.3. Rosa multiflora foliage contained higher concentrations of Ca, Mg, and K than K. latifolia, while K. latifolia foliage contained higher concentrations of Mn and Zn. Our research documents the importance of soil fertility as a predictor of the establishment of invasive and expansive shrubs. This study further shows that R. multiflora can establish and spread across a broader range of soil conditions than K. latifolia. C1 [Huebner, Cynthia D.] US Forest Serv, No Res Stn, USDA, Morgantown, WV 26505 USA. [Steinman, Jim] US Forest Serv, USDA, Newtown Sq, PA 19073 USA. [Hutchinson, Todd F.] US Forest Serv, No Res Stn, USDA, Delaware, OH 43015 USA. [Ristau, Todd E.; Royo, Alejandro A.] US Forest Serv, No Res Stn, USDA, Irvine, PA 16329 USA. RP Huebner, CD (reprint author), US Forest Serv, No Res Stn, USDA, 180 Canfield St, Morgantown, WV 26505 USA. EM chuebner@fs.fed.us FU 2-year Forest Health Monitoring-Evaluation Monitoring Grant, Northeastern Area State and Private Forestry, USDA Forest Service FX This research was funded by a 2-year Forest Health Monitoring-Evaluation Monitoring Grant, Northeastern Area State and Private Forestry, USDA Forest Service. We thank M. B. Adams, E. Allen, C. Moser, and three anonymous reviewers for their feedback. We also thank M. Ort and C. Coon for aid in site selection of sites in Ohio as well as H. Smith, L. Strickler, A. Gilbert, J. Quinn, E. Overton, M. Akers, and P. Green for their assistance in field sampling and data entry. NR 85 TC 4 Z9 4 U1 14 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD APR PY 2014 VL 377 IS 1-2 BP 259 EP 276 DI 10.1007/s11104-013-2000-x PG 18 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AD9WF UT WOS:000333614600018 ER PT J AU Matonyei, TK Cheprot, RK Liu, J Pineros, MA Shaff, JE Gudu, S Were, B Magalhaes, JV Kochian, LV AF Matonyei, T. K. Cheprot, R. K. Liu, J. Pineros, M. A. Shaff, J. E. Gudu, S. Were, B. Magalhaes, J. V. Kochian, L. V. TI Physiological and molecular analysis of aluminum tolerance in selected Kenyan maize lines SO PLANT AND SOIL LA English DT Article DE Aluminum toxicity; Aluminum tolerance; Root apex aluminum concentration; Root citrate exudation ID TRITICUM-AESTIVUM L; TROPICAL MAIZE; MALATE TRANSPORTER; ACTIVATED CITRATE; SOIL ACIDITY; ROOT-TIPS; RESISTANCE; PLANTS; WHEAT; TOXICITY AB Aluminum (Al) toxicity is an important limitation to maize production in many tropical and sub-tropical acid soil areas. The aim of this study was to survey the variation in Al tolerance in a panel of maize lines adapted for Kenya and look for novel sources of Al tolerance. 112 Kenyan maize accessions were phenotyped for Al tolerance in solution culture. Several Al tolerance-related parameters including relative net root growth (RNRG), root apex Al accumulation, Al-activated root organic acid exudation, and expression of the maize Al tolerance gene, ZmMATE1, were used to classify Kenyan maize accessions. Based on RNRG, 42 %, 28 %, and 30 % of the lines were classified as highly tolerant, moderately tolerant and sensitive, respectively. Tolerant accessions accumulated less Al in their root apices compared to sensitive lines. The Kenyan maize line, CON 5, and the Brazilian standard for tolerance, Cateto, exhibited the greatest Al tolerance based on RNRG, but CON 5 had only about 50 % of ZmMATE1 gene expression relative to Cateto. CON 5 also had low root apex Al content and high citrate exudation, suggesting that it may employ a citrate transporter other than ZmMATE1. We identified a very Al tolerant Kenyan maize line whose Al tolerance may be based in part on a novel tolerance gene. The maize lines identified in this study are useful germplasm for the development of varieties suitable for agriculture on acid soils in Kenya. C1 [Matonyei, T. K.; Cheprot, R. K.] Univ Eldoret, Eldoret, Kenya. [Liu, J.; Pineros, M. A.; Shaff, J. E.; Kochian, L. V.] Cornell Univ, Robert Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. [Gudu, S.; Were, B.] Rongo Univ Coll, Rongo, Kenya. [Magalhaes, J. V.] Embrapa Maize & Sorghum, Sete Lagoas, MG, Brazil. RP Kochian, LV (reprint author), Cornell Univ, Robert Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. EM lvk1@cornell.edu OI Kochian, Leon/0000-0003-3416-089X; Pineros, Miguel/0000-0002-7166-1848 FU Generation Challenge Program (GCP); McKnight Foundation FX The authors wishes to acknowledge the Generation Challenge Program (GCP) and McKnight Foundation for funding, Moi University for facilities and technical support, the USDA-ARS Robert Holley Center for Agriculture and Health for the mentoring of the first author and the provision of laboratory and technical support. We also wish to thank Drs. Lyza Maron and Michael Rutzke for their technical expertise and to Eric Craft for having worked tirelessly to ensure all the resources were available for use in the testing laboratory. Special thanks go to KARI-Kitale for providing some of the seed for Kenyan accessions. NR 43 TC 2 Z9 4 U1 1 U2 29 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD APR PY 2014 VL 377 IS 1-2 BP 357 EP 367 DI 10.1007/s11104-013-1976-6 PG 11 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AD9WF UT WOS:000333614600024 ER PT J AU Azhaguvel, P Mornhinweg, D Vidya-Saraswathi, D Rudd, JC Chekhovskiy, K Saha, M Close, TJ Dahleen, LS Weng, YQ AF Azhaguvel, Perumal Mornhinweg, Dolores Vidya-Saraswathi, Dhanasekaran Rudd, Jackie C. Chekhovskiy, Konstantin Saha, Malay Close, Timothy J. Dahleen, Lynn S. Weng, Yiqun TI Molecular mapping of greenbug (Schizaphis graminum) resistance gene Rsg1 in barley SO PLANT BREEDING LA English DT Article DE molecular markers; Hordeum vulgare; aphid resistance ID RUSSIAN WHEAT APHID; BRACHYPODIUM-DISTACHYON; CONFERS RESISTANCE; BIOTYPIC DIVERSITY; AEGILOPS-TAUSCHII; PLANT-RESISTANCE; MOSAIC-VIRUS; LINKAGE MAP; GENOME; REGISTRATION AB The greenbug, Schizaphis graminum (Rondani) is an extremely damaging aphid pest of barley (Hordeum vulgare L.) particularly in the southern Great Plains of the USA. The simply inherited, dominant resistance gene Rsg1 is in all greenbug-resistant US barley cultivars. In this study, we conducted molecular mapping of Rsg1 using an F-2:3 population derived from a cross between the greenbug-resistant Post 90*4/R015 and susceptible CI2260 inbred lines. Segregation of host responses to greenbug biotype E infestation confirmed that a single dominant gene is responsible for greenbug resistance in Post 90*4/R015. Simple sequence repeat (SSR) markers evenly distributed along the seven barley chromosomes were employed for the construction of a framework genetic map. Linkage analysis placed the Rsg1 locus in the long arm of chromosome 3H (3HL) flanked by SSR markers Bmag0877 and GBM1420 that were 35cM apart. Polymorphic single-nucleotide polymorphism (SNP) markers in 3HL were identified from an Illumina GoldenGate SNP assay and used for targeted mapping to locate Rsg1 to an 8.4-cM interval. Comparative analysis identified syntenic genomic regions in Brachypodium distachyon chromosome 2, in which 37 putative genes were annotated including a NB-LRR-type resistance gene homologue that may be a potential candidate gene for the Rsg1 locus of barley. Results from this study offer a starting point for fine mapping and cloning of this aphid resistance gene in barley. C1 [Azhaguvel, Perumal; Vidya-Saraswathi, Dhanasekaran; Rudd, Jackie C.; Weng, Yiqun] Texas AgriLife Res, Amarillo, TX 79106 USA. [Azhaguvel, Perumal; Chekhovskiy, Konstantin; Saha, Malay] Samuel Roberts Noble Fdn Inc, Ardmore, OK 73401 USA. [Mornhinweg, Dolores] ARS, USDA, Wheat Peanut & Other Field Crops Res Unit, Stillwater, OK 74075 USA. [Close, Timothy J.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Dahleen, Lynn S.] ARS, USDA, Cereal Crops Res Unit, Red River Valley Agr Res Ctr, Fargo, ND 58102 USA. [Weng, Yiqun] Univ Wisconsin, Dept Hort, USDA, ARS, Madison, WI 53706 USA. RP Weng, YQ (reprint author), Texas AgriLife Res, 6500 Amarillo Blvd W, Amarillo, TX 79106 USA. EM do.mornhinweg@ars.usda.gov; yiqun.weng@ars.usda.gov FU USDA-NRI (CSREES) [2006-35301-16892]; Wheat MAS Project (CAP) [2006-55606-16629] FX This study was supported by the USDA-NRI grant (CSREES# 2006-35301-16892) to Y. Weng and partly supported by the Wheat MAS Project (CAP# 2006-55606-16629) to J. Rudd. The authors are grateful to Peihuan Yan and Jony Simmons for technical support. NR 64 TC 0 Z9 0 U1 1 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD APR PY 2014 VL 133 IS 2 BP 227 EP 233 DI 10.1111/pbr.12143 PG 7 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA AE1UR UT WOS:000333756500009 ER PT J AU Cuevas, HE Prom, LK Erpelding, JE Brotons, V AF Cuevas, Hugo E. Prom, Louis K. Erpelding, John E. Brotons, Veronica TI Assessments of genetic diversity and anthracnose disease response among Zimbabwe sorghum germplasm SO PLANT BREEDING LA English DT Article DE exotic germplasm; anthracnose; genetic diversity; simple sequence repeats; Sorghum bicolor ID L. MOENCH ACCESSIONS; SSR MARKERS; MICROSATELLITE MARKERS; BICOLOR; GRAIN; LANDRACES; ASSOCIATION; COLLECTION; RESISTANCE; SEVERITY AB The USDA-ARS National Plant Germplasm System maintains a Zimbabwe sorghum collection of 1235 accessions from different provinces. This germplasm has not been extensively employed in US breeding programmes due to the lack of phenotypic and genetic characterization. Therefore, 68 accessions from Zimbabwe were phenotyped, and evaluated for their anthracnose response for two consecutive years, and genetically characterized with 21 simple sequence repeat markers. Phenotypic analysis showed significant differences among accessions with plant height and panicle length being the most variable traits. Likewise, 25 accessions were anthracnose resistant, nine showed variable responses and 34 were susceptible. Genetic analysis identified 174 alleles with an average of 8.3 alleles and 11.8 genotypes per locus and a polymorphic information content of 0.60. These results reflect a moderate genetically diverse germplasm. Neighbour-joining clustering analysis revealed that the majority of anthracnose-resistant accessions showed high genetic relatedness; therefore, this germplasm might represent one to six new sources of resistances. Results presented herein show that the Zimbabwe collection contains valuable germplasm for breeding programmes and is an important source of anthracnose resistance. C1 [Cuevas, Hugo E.; Erpelding, John E.] ARS, Trop Agr Res Stn, USDA, Mayaguez, PR 00680 USA. [Prom, Louis K.] ARS, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. [Erpelding, John E.] ARS, USDA, Mid South Area, Stoneville, MS 38776 USA. [Brotons, Veronica] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR 00681 USA. RP Cuevas, HE (reprint author), ARS, Trop Agr Res Stn, USDA, 2200 Pedro Albizu Campos Ave, Mayaguez, PR 00680 USA. EM hugo.cuevas@ars.usda.gov NR 48 TC 4 Z9 4 U1 0 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD APR PY 2014 VL 133 IS 2 BP 234 EP 242 DI 10.1111/pbr.12133 PG 9 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA AE1UR UT WOS:000333756500010 ER PT J AU Jan, CC Seiler, GJ Hammond, JJ AF Jan, Chao-Chien Seiler, Gerald J. Hammond, Jim J. TI Effect of wild Helianthus cytoplasms on agronomic and oil characteristics of cultivated sunflower (Helianthus annuus L.) SO PLANT BREEDING LA English DT Article DE alloplasmic; euplasmic; isogenic lines; Helianthus species; interspecific hybridization; cytoplasmic substitution; cytoplasmic male sterility ID REGISTRATION; GERMPLASM; TRAITS AB Sunflower (Helianthus annuus L.) productions reliance on a single source of cytoplasmic male sterility, PET1, derived from H.petiolaris Nutt., makes the crop genetically vulnerable. Twenty diverse cytoplasmic substitution lines from annual and perennial wild species were compared with the inbred line HA89 over four environments. Pairwise comparisons of alloplasmic male fertile (MF) with euplasmic MF lines suggested that cytoplasm of perennial H.angustifolius increased lodging while perennial cytoplasms of H.mollis, H.grosseserratus, and H.divaricatus reduced head size and consequently reduced yield and will need to be used with caution. Lines having annual species, cytoplasms had no effects on agronomic traits. Comparisons of CMS HA 89 with 10 CMS lines suggested yield-reducing cytoplasmic effects of perennial H.maximiliani and annual H.annuus PI 413178 and PI 413024. Cytoplasmic effects on oil percentage and fatty acid composition were minimal and should not be of concern for sunflower breeders. In general, most cytoplasms of wild annual Helianthus species accommodate cultivated nuclear genes without drastic adverse interactions, and are potential sources of cytoplasmic diversity for sunflower breeding. C1 [Jan, Chao-Chien; Seiler, Gerald J.] ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. [Hammond, Jim J.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Jan, CC (reprint author), ARS, USDA, No Crop Sci Lab, Fargo, ND 58102 USA. EM chaochien.jan@ars.usda.gov NR 18 TC 0 Z9 0 U1 2 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0179-9541 EI 1439-0523 J9 PLANT BREEDING JI Plant Breed. PD APR PY 2014 VL 133 IS 2 BP 262 EP 267 DI 10.1111/pbr.12151 PG 6 WC Agronomy; Biotechnology & Applied Microbiology; Plant Sciences SC Agriculture; Biotechnology & Applied Microbiology; Plant Sciences GA AE1UR UT WOS:000333756500014 ER PT J AU Frett, TJ Reighard, GL Okie, WR Gasic, K AF Frett, Terrence J. Reighard, Gregory L. Okie, William R. Gasic, Ksenija TI Mapping quantitative trait loci associated with blush in peach [Prunus persica (L.) Batsch] SO TREE GENETICS & GENOMES LA English DT Article DE QTLs; Anthocyanin; Candidate gene ID MYB TRANSCRIPTION FACTORS; RED SKIN COLOR; PHENOLIC-COMPOUNDS; ANTIOXIDANT ACTIVITY; ANTHOCYANIN BIOSYNTHESIS; PLUM GENOTYPES; FRUIT-QUALITY; FREESTONE PEACHES; GROUND COLOR; GENE AB Blush is an important trait for marketing peaches. The red skin pigmentation develops through the flavonoid and anthocyanin pathways, and both genetic and environmental stimuli, and their interaction, control the regulation of these pathways. The molecular basis of blush development in peach is yet to be understood. An F-2 blush population (ZC(2)) derived from a cross between two peach cultivars with contrasting phenotypes for blush, "Zin Dai" (similar to 30 % red) and "Crimson Lady" (similar to 100 % red), was used for linkage map construction and quantitative trait loci (QTLs) mapping. The segregating population was phenotyped for blush for 4 years using a visual rating scale and quantified using a colorimeter (L*, a*, and b*) 1 year. The ZC(2) population was genotyped with the IPSC 9 K peach single-nucleotide polymorphism (SNP) array v1, and a high-density ZC(2) genetic linkage map was constructed. The map covers genetic a distance of similar to 452.51 cM with an average marker spacing of 2.38 cM/marker. Four QTLs were detected: one major QTL on LG3 (Blush.Pp.ZC-3.1) and three minor QTLs on LG 4 and 7 (Blush.Pp.ZC-4.1; Blush.Pp.ZC-4.2; Blush.Pp.ZC-7.1), indicating the presence of major and minor genes involved in blush development. Candidate genes involved in skin and flesh coloration of peach (PprMYB10), cherry (PavMYB10), and apple (MdMYB1/MdMYBA/MdMYB10) are located within the interval of the major QTL on LG3, suggesting the same genetic control for color development in the Rosaceae family. Marker-assisted selection (MAS) for blush is discussed. C1 [Frett, Terrence J.; Reighard, Gregory L.; Gasic, Ksenija] Clemson Univ, SAFES, Clemson, SC 29634 USA. [Okie, William R.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Gasic, K (reprint author), Clemson Univ, SAFES, Clemson, SC 29634 USA. EM kgasic@clemson.edu FU NIFA/USDA [SC-1700382]; USDA's National Institute of Food and Agriculture-Specialty Crop Research Initiative project [2009-51181-05808] FX This work was supported by NIFA/USDA under project number SC-1700382 and technical contribution No. 6099 of the Clemson University Experiment Station. This work was partially funded by USDA's National Institute of Food and Agriculture-Specialty Crop Research Initiative project, "RosBREED: Enabling marker-assisted breeding in Rosaceae" (2009-51181-05808). NR 78 TC 14 Z9 14 U1 3 U2 56 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1614-2942 EI 1614-2950 J9 TREE GENET GENOMES JI Tree Genet. Genomes PD APR PY 2014 VL 10 IS 2 BP 367 EP 381 DI 10.1007/s11295-013-0692-y PG 15 WC Forestry; Genetics & Heredity; Horticulture SC Forestry; Genetics & Heredity; Agriculture GA AD4ZO UT WOS:000333261100013 ER PT J AU Flax, VL Bentley, ME Combs, GF Chasela, CS Kayira, D Tegha, G Kamwendo, D Daza, EJ Fokar, A Kourtis, AP Jamieson, DJ van der Horst, CM Adair, LS AF Flax, Valerie L. Bentley, Margaret E. Combs, Gerald F., Jr. Chasela, Charles S. Kayira, Dumbani Tegha, Gerald Kamwendo, Debbie Daza, Eric J. Fokar, Ali Kourtis, Athena P. Jamieson, Denise J. van der Horst, Charles M. Adair, Linda S. TI Plasma and breast-milk selenium in HIV-infected Malawian mothers are positively associated with infant selenium status but are not associated with maternal supplementation: results of the Breastfeeding, Antiretrovirals, and Nutrition study SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID GLUTATHIONE-PEROXIDASE ACTIVITY; PREGNANT-WOMEN; GENITAL-TRACT; BLOOD; ZINC; TUBERCULOSIS; DEFICIENCY; LACTATION; MORTALITY; CHILDREN AB Background: Selenium is found in soils and is essential for human antioxidant defense and immune function. In Malawi, low soil selenium and dietary intakes coupled with low plasma selenium concentrations in HIV infection could have negative consequences for the health of HIV-infected mothers and their exclusively breastfed infants. Objective: We tested the effects of lipid-based nutrient supplements (LNS) that contained 1.3 times the Recommended Dietary Allowance of sodium selenite and antiretroviral drugs (ARV) on maternal plasma and breast-milk selenium concentrations. Design: HIV-infected Malawian mothers in the Breastfeeding, Antiretrovirals, and Nutrition study were randomly assigned at delivery to receive: LNS, ARV, LNS and ARV, or a control. In a subsample of 526 mothers and their uninfected infants, we measured plasma and breast-milk selenium concentrations at 2 or 6 (depending on the availability of infant samples) and 24 wk postpartum. Results: Overall, mean (+/- SD) maternal (range: 81.2 6 20.4 to 86.2 6 19.9 mu g/ L) and infant (55.6 +/- 16.3 to 61.0 +/- 15.4 mu g/L) plasma selenium concentrations increased, whereas breast-milk selenium concentrations declined (14.3 +/- 11.5 to 9.8 +/- 7.3 mu g/L) from 2 or 6 to 24 wk postpartum (all P < 0.001). Compared with the highest baseline selenium tertile, low and middle tertiles were positively associated with a change in maternal plasma or breast-milk selenium from 2 or 6 to 24 wk postpartum (both P < 0.001). With the use of linear regression, we showed that LNS that contained selenium and ARV were not associated with changes in maternal plasma and breast-milk selenium, but maternal selenium concentrations were positively associated with infant plasma selenium at 2 or 6 and 24 wk postpartum (P < 0.001) regardless of the study arm. Conclusions: Selenite supplementation of HIV-infected Malawian women was not associated with a change in their plasma or breast-milk selenium concentrations. Future research should examine effects of more readily incorporated forms of selenium (ie, selenomethionine) in HIV-infected breastfeeding women. This trial was registered at clinicaltrials. gov as NCT00164736. C1 [Flax, Valerie L.; Bentley, Margaret E.; Daza, Eric J.; Adair, Linda S.] Univ N Carolina, Carolina Populat Ctr, Sch Med, Chapel Hill, NC 27516 USA. [Flax, Valerie L.; Bentley, Margaret E.; Adair, Linda S.] Univ N Carolina, Dept Nutr, Sch Med, Chapel Hill, NC 27516 USA. [Daza, Eric J.] Univ N Carolina, Dept Biostat, Sch Med, Chapel Hill, NC 27516 USA. [Fokar, Ali; van der Horst, Charles M.] Univ N Carolina, Div Infect Dis, Sch Med, Chapel Hill, NC 27516 USA. [Combs, Gerald F., Jr.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND USA. [Chasela, Charles S.; Kayira, Dumbani; Tegha, Gerald; Kamwendo, Debbie] Univ North Carolina Project, Lilongwe, Malawi. [Chasela, Charles S.] Univ Witwatersrand, Sch Publ Hlth, Div Epidemiol & Biostat, Parktown, South Africa. [Kourtis, Athena P.; Jamieson, Denise J.] US CDC, Atlanta, GA USA. RP Flax, VL (reprint author), Univ N Carolina, Carolina Populat Ctr, Campus Box 8120, Chapel Hill, NC 27516 USA. EM flax@unc.edu OI Flax, Valerie/0000-0003-0200-3355 FU Bill & Melinda Gates Foundation [OPP53107]; National Institute of Allergy and Infectious Diseases; University of North Carolina Center for AIDS Research [P30-AI50410]; Carolina Population Center [R24 HD050924]; NIH Fogarty AIDS International Training and Research Program (DHHS/NIH/FIC) [2-D43 TW001039, R24TW007988]; Elizabeth Glaser Pediatric AIDS Foundation; United Nations Children's Fund; World Food Program; Malawi Ministry of Health and Population; Johnson Johnson; US Agency for International Development; Prevention Research Centers Special Interest Project of the CDC [SIP 13-01 U48-CCU409660-09, SIP 26-04 U48-DP000059-01, SIP 22-09 U48-DP001944-01] FX The Breastfeeding, Antiretrovirals, and Nutrition (BAN) study was supported by grants from the Prevention Research Centers Special Interest Project of the CDC (SIP 13-01 U48-CCU409660-09, SIP 26-04 U48-DP000059-01, and SIP 22-09 U48-DP001944-01), the Bill & Melinda Gates Foundation (OPP53107), the National Institute of Allergy and Infectious Diseases, the University of North Carolina Center for AIDS Research (P30-AI50410), the Carolina Population Center (R24 HD050924), and the NIH Fogarty AIDS International Training and Research Program (DHHS/NIH/FIC 2-D43 TW001039 and R24TW007988; the American Recovery and Reinvestment Act). The Call to Action Prevention of Mother-To-Child Transmission program, from which BAN mothers were recruited, was supported by the Elizabeth Glaser Pediatric AIDS Foundation, the United Nations Children's Fund, the World Food Program, the Malawi Ministry of Health and Population, Johnson & Johnson, and the US Agency for International Development. Antiretrovirals used in the BAN study were donated by Abbott Laboratories, GlaxoSmithKline, Boehringer Ingelheim, Roche Pharmaceuticals, and Bristol-Myers Squibb. NR 47 TC 1 Z9 1 U1 0 U2 12 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD APR PY 2014 VL 99 IS 4 BP 950 EP 956 DI 10.3945/ajcn.113.073833 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AD3VA UT WOS:000333173100023 PM 24500152 ER PT J AU Krugner, R AF Krugner, Rodrigo TI Suitability of non-fertilized eggs of Homalodisca vitripennis for the egg parasitoid Gonatocerus morrilli SO BIOCONTROL LA English DT Article DE Glassy-winged sharpshooter; Hemiptera; Cicadellidae; Hymenoptera; Mymaridae; Non-fertilized host eggs ID GLASSY-WINGED SHARPSHOOTER; HEMIPTERA CICADELLIDAE; SOUTHERN CALIFORNIA; COAGULATA HEMIPTERA; ASHMEADI HYMENOPTERA; PIERCES-DISEASE; GIRAULT HYMENOPTERA; MYMARIDAE; SPP.; PATTERNS AB Gonatocerus morrilli (Howard) (Hymenoptera: Mymaridae) is an egg parasitoid used in California, USA to control glassy-winged sharpshooter (GWSS), Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae). Virgin GWSS females deposit non-fertilized eggs and mated females can exhaust sperm reserves for egg fertilization. However, nothing is known about Gonatocerus spp. performance when using non-fertilized GWSS eggs as hosts. Host age preference for oviposition and suitability of non-fertilized GWSS eggs as hosts for G. morrilli reproduction were investigated to determine whether non-fertilized eggs on sentinel plants could be used to monitor egg parasitoid populations. Gonatocerus morrilli parasitized all ages of GWSS eggs (1-8 days old) regardless if the host egg was fertilized or not. In choice tests (fertilized versus non-fertilized eggs), parasitoids failed to emerge as adults from non-fertilized eggs more often than from fertilized eggs. The results indicate that non-fertilized eggs were accepted by G. morrilli as suitable hosts for oviposition, but were less suitable for immature development compared to fertilized eggs. C1 ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Krugner, R (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM rodrigo.krugner@ars.usda.gov NR 28 TC 2 Z9 2 U1 2 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1386-6141 EI 1573-8248 J9 BIOCONTROL JI Biocontrol PD APR PY 2014 VL 59 IS 2 BP 167 EP 174 DI 10.1007/s10526-014-9562-2 PG 8 WC Entomology SC Entomology GA AD4EK UT WOS:000333199600004 ER PT J AU Klooster, WS Herms, DA Knight, KS Herms, CP McCullough, DG Smith, A Gandhi, KJK Cardina, J AF Klooster, Wendy S. Herms, Daniel A. Knight, Kathleen S. Herms, Catherine P. McCullough, Deborah G. Smith, Annemarie Gandhi, Kamal J. K. Cardina, John TI Ash (Fraxinus spp.) mortality, regeneration, and seed bank dynamics in mixed hardwood forests following invasion by emerald ash borer (Agrilus planipennis) SO BIOLOGICAL INVASIONS LA English DT Article DE Ash regeneration; Demography; Invasive forest pest; Seedlings ID COLEOPTERA BUPRESTIDAE; SOUTHEASTERN MICHIGAN; DECIDUOUS FOREST; NORTH-AMERICAN; SUCCESSION; MINNESOTA; PATHOGENS; ECOSYSTEM; DIEBACK; STANDS AB Emerald ash borer (EAB; Agrilus planipennis) has killed millions of ash trees and threatens ash throughout North America, and long-term persistence of ash will depend on the potential for regeneration. We quantified ash demography, including mortality and regeneration, of Fraxinus americana (white ash), Fraxinus pennsylvanica (green ash), and Fraxinus nigra (black ash) in mixed hardwood forests near the epicenter of the EAB invasion in southeastern Michigan and throughout Ohio. Plots were established across a gradient of ash densities. Ash was the most important species in hydric sites, and ranked second among all species in mesic and xeric sites. In sites nearest the epicenter in Michigan, ash mortality exceeded 99 % by 2009, and few or no newly germinated ash seedlings were observed, leaving only an "orphaned cohort" of established ash seedlings and saplings. As ash mortality increased, the number of viable ash seeds in soil samples decreased sharply, and no viable seeds were collected in 2007 or 2008. In Ohio sites farther from the epicenter, densities of new ash seedlings were much higher in plots with healthy ash trees compared to plots where trees had died. EAB was still present in low densities in Michigan and Ohio stands in 2012 where average mortality of ash was nearly 100 %. The future of ash at these sites will depend on the outcome of the dynamic interaction between the orphaned cohort of previously established ash seedlings and saplings and low density EAB populations. C1 [Klooster, Wendy S.; Herms, Catherine P.; Cardina, John] Ohio State Univ, Dept Hort & Crop Sci, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Herms, Daniel A.; Smith, Annemarie; Gandhi, Kamal J. K.] Ohio State Univ, Dept Entomol, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. [Knight, Kathleen S.] US Forest Serv, Forestry Sci Lab, No Res Stn, USDA, Delaware, OH 43015 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. RP Klooster, WS (reprint author), Ohio State Univ, Dept Hort & Crop Sci, Ohio Agr Res & Dev Ctr, 1680 Madison Ave, Wooster, OH 44691 USA. EM klooster.2@osu.edu FU USDA Forest Service Northeastern Research Station's Research on Biological Invasions of Northeastern Forests program; USDA National Research Initiative Biology of Weedy and Invasive Species in Agroecosystems competitive grants program; National Institute of Food and Agriculture; Cooperative Agreements with the USDA Forest Service Northern Research Station, Delaware, OH; state and federal funds appropriated to the Ohio Agricultural Research and Development Center; Ohio State University; Cooperative Agreements with the USDA Forest Service Northern Research Station, Delaware, OH FX For their extensive assistance with field and laboratory work we thank Limbania Aliega, Lourdes Arrueta, Stephanie Blumer, Alejandra Claure, Christian Colindres, Kyle Costilow, Tara Dell, Mary Douglas, Eileen Duarte, Charles Flower, Stefanie Fluke, Tim Fox, Joan Jolliffas, Lawrence Long, Rina Mejia, Rachel Nieswander, Sarahi Nunez, Delmy Sanchez, Sebastian Saenz, Stephanie Smith, and Nathan Yaussy, as well as other members of the Herms, Knight and McCullough Labs. We are especially grateful to Diane Hartzler who organized and coordinated much of the field work in Michigan since the inception of the study. We also thank the two anonymous reviewers for their constructive suggestions that helped us improve this manuscript. This research was funded by grants from the USDA Forest Service Northeastern Research Station's Research on Biological Invasions of Northeastern Forests program; USDA National Research Initiative Biology of Weedy and Invasive Species in Agroecosystems competitive grants program; the National Institute of Food and Agriculture; Cooperative Agreements with the USDA Forest Service Northern Research Station, Delaware, OH; and state and federal funds appropriated to the Ohio Agricultural Research and Development Center and The Ohio State University. NR 56 TC 31 Z9 33 U1 31 U2 163 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD APR PY 2014 VL 16 IS 4 BP 859 EP 873 DI 10.1007/s10530-013-0543-7 PG 15 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AD1RR UT WOS:000333011700011 ER PT J AU Kleist, A Herrera-Reddy, AM Sforza, R Jasieniuk, M AF Kleist, Annabelle Herrera-Reddy, Angelica M. Sforza, Rene Jasieniuk, Marie TI Inferring the complex origins of horticultural invasives: French broom in California SO BIOLOGICAL INVASIONS LA English DT Article DE French broom; Genista monspessulana; Horticultural invasives; Introduction history; Microsatellite markers ID APPROXIMATE BAYESIAN COMPUTATION; POPULATION-STRUCTURE; MEDITERRANEAN BASIN; MICROSATELLITE DATA; BIOLOGICAL-CONTROL; GENETIC-VARIATION; EVOLUTION; SOFTWARE; HYBRIDIZATION; HISTORY AB Investigating the origins of invasive populations provides insight into the evolutionary and anthropogenic factors underlying invasions, and can inform management decisions. Invasive species introduced for horticultural purposes often have complex origins typified by multiple introductions of species, cultivars, and genotypes, and interspecific and intraspecific hybridizations in introduced ranges. Such complex introduction histories may result in complex genetic signatures in the invaded range, making inferences about origins difficult, particularly when all putative sources cannot be sampled. In this study, we inferred the origins of the invasive French broom complex in California using 12 nuclear microsatellite markers. We characterized the genetic diversity and population structure of invasive and horticultural brooms in their invaded range in California and of Genista monspessulana in its native Mediterranean range. Overall, no significant differences in allelic richness, observed heterozygosity, inbreeding, or genetic structure were observed between the invaded and native ranges, but differences existed among populations within ranges. Bayesian STRUCTURE analysis revealed three genetic clusters in the French broom complex. Nearly all native G. monspessulana assigned highly to a single cluster. Many invasives assigned to a second cluster that contained Genista canariensis, Genista stenopetala, and ornamental sweet broom, and the remaining invasives assigned to a third cluster that also contained some G. monspessulana individuals from Sardinia and Corsica. Admixture between the second and third clusters was detected. Approximate Bayesian Computation analysis of six alternative scenarios supported the hypothesis that some invasive French broom is derived from an unsampled population branching from ornamental sweet broom. A combination of factors, including multiple introductions, escapes from cultivation, and inter-taxon hybridization, likely contribute to the invasive success of French broom in California and may have important implications for management, in particular biological control. C1 [Kleist, Annabelle; Jasieniuk, Marie] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Herrera-Reddy, Angelica M.] ARS, Exot & Invas Weeds Res Unit, USDA, Albany, CA 94710 USA. [Sforza, Rene] USDA ARS, European Biol Control Lab, F-34988 St Gely Du Fesc, France. RP Kleist, A (reprint author), Univ Calif Davis, Dept Plant Sci, Mail Stop 4, Davis, CA 95616 USA. EM ackleist@ucdavis.edu FU United States Department of Agriculture; Henry A. Jastro Research Scholarships; California Weed Science Society; Department of Plant Sciences Graduate Student Research award FX Annabelle Kleist was supported by a Department of Plant Sciences Graduate Student Research award. Financial support for the research from the United States Department of Agriculture, Henry A. Jastro Research Scholarships, and the California Weed Science Society is gratefully acknowledged. Trammie Bui and Yuan Qin provided lab assistance. We thank Carla D'Antonio, UC Botanical Garden, Los Angeles County Arboretum and Botanic Garden, Desert Legume Program, Okologisch-Botanischer Garten der Universitat Bayreuth, Monrovia Nursery, RZ Nursery, Walter Andersen Nursery, and Merriments Gardens for plant specimens. We also thank Joseph DiTomaso for introducing us to the study system, and Libby Karn, Miki Okada, Dan Potter, and Kevin Rice for useful comments on the manuscript. NR 55 TC 2 Z9 2 U1 6 U2 56 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD APR PY 2014 VL 16 IS 4 BP 887 EP 901 DI 10.1007/s10530-013-0546-4 PG 15 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AD1RR UT WOS:000333011700013 ER PT J AU Rivas, FA Tabatabai, MA Olk, DC Thompson, ML AF Rivas, F. A. Tabatabai, M. A. Olk, D. C. Thompson, M. L. TI Kinetics of short-term carbon mineralization in roots of biofuel crops in soils SO BIOLOGY AND FERTILITY OF SOILS LA English DT Article DE Roots; Decomposition; Biofuel crops; CO2; Rate constant; Cropping systems ID DECOMPOSITION; RESIDUES; LIGNIN; NITROGEN; PLANTS; STABILIZATION; MECHANISMS AB Various crops have been used for energy production while striving to maintain C storage derived from roots in soils. To better understand and document the rates of root decomposition in biofuel cropping systems, we compared the evolution of CO2 from roots incubated with samples of two Iowa Mollisols (Clarion and Nicollet). Root samples were collected from experimental plots for four cropping systems: a multispecies reconstructed prairie, grown with and without N fertilization, and continuous corn, grown with and without a ryegrass cover crop. Major structural components of the root samples (lignin, cellulose, and hemicellulose) as well as chemical composition (total C and N) were assessed. The root materials were incubated for 30 days at room temperature (22 A degrees C) by using an incubation apparatus with continuous airflow. The decomposition rates and half-lives of rapidly and slowly decomposable C fractions were calculated by fitting a two-component first-order kinetic model to the data. Mineralizable C ranged from 7 to 13 % of the added C. When the data for the two soils were combined, the CO2-C evolved was positively correlated with both the C/N ratio (r = 0.88**, p < 0.01) and the lignin/N ratio (r = 0.89**, p < 0.01) of the roots. For the Clarion soil, first-order decomposition rate constants for the rapid fraction ranged from 0.07 to 0.69 day(-1), whereas for the Nicollet soil they ranged from 0.09 to 0.66 day(-1). For both soils, the half-lives of the rapidly decomposable fraction ranged from 1 to 10 days, and half-lives of 204 to 770 days were observed for the slowly decomposable fractions. Among the cropping systems studied, the rapidly decomposable fraction of roots derived from the unfertilized prairie treatment was the largest. Those root residues also had the highest hemicellulose index and higher concentrations of arabinose, galactose, glucose, and xylose sugars than did roots of the other crops. Other decomposition parameters, such as the decomposition rates and half-lives obtained from the two-component model, were not correlated with the root composition parameters studied. Our results suggest that lignin did not inhibit the early rate of root C mineralization in mixed perennial crops. That information could be useful in refining models of root C dynamics in daily time steps or at the scale of a single growing season. C1 [Rivas, F. A.; Tabatabai, M. A.; Thompson, M. L.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Olk, D. C.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Thompson, ML (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM mlthomps@iastate.edu FU Plant Sciences Institute at Iowa State University; Carbon Cycle Science Program of the National Institute for Food and Agriculture, US Department of Agriculture FX We gratefully acknowledge the technical support of T. Grimard and T. Chua in determination of acid-extractable monosaccharides and lignin, respectively. We thank the editor and two anonymous reviewers for their suggestions to improve the manuscript. The project was funded by the Plant Sciences Institute at Iowa State University and by the Carbon Cycle Science Program of the National Institute for Food and Agriculture, US Department of Agriculture. NR 35 TC 2 Z9 2 U1 1 U2 34 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0178-2762 EI 1432-0789 J9 BIOL FERT SOILS JI Biol. Fertil. Soils PD APR PY 2014 VL 50 IS 3 BP 527 EP 535 DI 10.1007/s00374-013-0870-y PG 9 WC Soil Science SC Agriculture GA AD1VW UT WOS:000333022800011 ER PT J AU Sloat, MR Reeves, GH AF Sloat, Matthew R. Reeves, Gordon H. TI Individual condition, standard metabolic rate, and rearing temperature influence steelhead and rainbow trout (Oncorhynchus mykiss) life histories SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID SALMON SALMO-SALAR; JUVENILE ATLANTIC SALMON; BROWN TROUT; STREAM TEMPERATURE; SEXUAL-MATURATION; PARTIAL MIGRATION; WILD POPULATION; REACTION NORMS; TELEOST FISH; GROWTH AB We reared juvenile Oncorhychus mykiss with low and high standard metabolic rates (SMR) under alternative thermal regimes to determine how these proximate factors influence life histories in a partially migratory salmonid fish. High SMR significantly decreased rates of freshwater maturation and increased rates of smoltification in females, but not males, after 1 year of rearing. Warmer water temperatures significantly decreased rates of freshwater maturation and increased rates of smoltification in both sexes. Variation in individual growth influenced the probability of adopting anadromy or freshwater residency as life histories, but produced paradoxical results. Individuals with the highest growth performance within their respective temperature treatments had a higher probability of freshwater maturation, but warmer temperatures decreased freshwater maturation despite significantly increasing somatic growth. Whole-body lipid content was significantly lower for fish reared in the warm temperature treatment, which may explain the decreased probability of freshwater maturation for individuals exposed to warmer temperatures. Our results indicate that changes in somatic growth induced by altered thermal regimes can influence the relationship between body size and the probability of maturation. Accordingly, somatic growth may not be a robust predictor of shifts in the prevalence of anadromy and residency in partially migratory salmonids when compared across thermal regimes. C1 [Sloat, Matthew R.] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Reeves, Gordon H.] US Forest Serv, USDA, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Sloat, MR (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, 104 Nash Hall, Corvallis, OR 97331 USA. EM matthew.sloat@oregonstate.edu FU USDA Forest Service; Pacific Northwest Research Station, Corvallis, Oregon; National Marine Fisheries Service; Northwest Fisheries Science Center, Washington; Oregon Chapter of the American Fisheries Society Carl Bond Memorial Scholarship FX We thank staff at the Oregon Department of Fisheries and Wildlife (ODFW) Clackamas River Hatchery for help obtaining fish for this experiment. Brian Nielsen and India Sloat helped with the construction of the experimental streams. Jerri Bartholomew, Don Stevens, and Jill Pridgeon provided logistical support at Oregon State University (OSU) Salmon Disease Lab. Amy Lindsley, Haley Ohms, Matt Stinson, Rosie Gonzalez, and Stephanie Saunders helped with data collection. Funding for this work was provided by the USDA Forest Service, Pacific Northwest Research Station, Corvallis, Oregon, National Marine Fisheries Service, Northwest Fisheries Science Center, Washington, and the Oregon Chapter of the American Fisheries Society Carl Bond Memorial Scholarship. Early versions of the manuscript benefited from comments by Jason Dunham, David Noakes, Michael Blouin, Chris Jordan, Haley Ohms, and John McMillan. Kathryn Ronnenberg provided valuable graphical and editorial assistance. The use of animals in this experiment was conducted under OSU Animal Care and Use Permits Nos. 3923 and 4019. NR 64 TC 15 Z9 15 U1 9 U2 73 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD APR PY 2014 VL 71 IS 4 BP 491 EP 501 DI 10.1139/cjfas-2013-0366 PG 11 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AD7AD UT WOS:000333412000001 ER PT J AU Thomas-Van Gundy, M Rentch, J Adams, MB Carson, W AF Thomas-Van Gundy, Melissa Rentch, James Adams, Mary Beth Carson, Walter TI Reversing legacy effects in the understory of an oak-dominated forest SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE disturbance ecology; succession; deer browse; prescribed fire; regeneration ID EASTERN-UNITED-STATES; REPEATED PRESCRIBED FIRES; NORTHERN RED OAK; MOUNTAINS NATIONAL-PARK; WHITE-TAILED DEER; DECIDUOUS FOREST; STAND STRUCTURE; CANOPY GAPS; SOUTHERN APPALACHIANS; HARDWOOD REGENERATION AB Current forests developed under conditions different from original forests, with higher deer densities, reduced fire frequency, denser canopies, and smaller canopy gaps. These alterations have led to understories dominated by species simultaneously browse tolerant, shade tolerant, and fire sensitive leading to difficulties in the regeneration of oak species (Quercus spp.) in some areas. We evaluated how three key processes - understory fire, canopy gaps, and browsing - influenced tree species in east central West Virginia. We were particularly interested in the response of oak species because they are the dominant overstory species and of maple species (Acer spp.), black birch (Betula lenta L.), and yellow-poplar (Liriodendron tulipifera L.) as these are likely to replace the current forest. Single-process effects were significant and significant interactions among processes were numerous. In general, fire caused significant reductions of seedlings and saplings of red maple (Acer rubrum L.) and striped maple (Acer pensylvanicum L.) and increased the seedlings of black birch and yellow-poplar. Canopy gaps increased the abundance of black birch and yellow-poplar seedlings and saplings. Gaps and fire together caused an increase in the relative abundance of yellow-poplar. Excluding browsers and creating canopy gaps together nearly doubled oak sapling importance values versus either treatment alone; however, oak importance values remained low. Given the significant interactions of browse control with the other two processes, browse control should be considered when planning oak regeneration treatments such as canopy gaps or prescribed fire. C1 [Thomas-Van Gundy, Melissa; Adams, Mary Beth] USDA Forest Serv, No Res Stn, Parsons, WV 26287 USA. [Rentch, James] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Carson, Walter] Univ Pittsburgh, Dept Biol Sci, Pittsburgh, PA 15260 USA. RP Thomas-Van Gundy, M (reprint author), USDA Forest Serv, No Res Stn, POB 404, Parsons, WV 26287 USA. EM mthomasvangundy@fs.fed.us FU University of Pittsburgh [4300-FS-NE-4353-13]; USDA Forest Service Northern Research Station; USDA National Research Initiative Competitive Grant [99-35101-7732] FX Funding for this study (4300-FS-NE-4353-13) has come from the University of Pittsburgh, USDA Forest Service Northern Research Station, and USDA National Research Initiative Competitive Grant 99-35101-7732 to Walter Carson. We are grateful to Rachel Collins for setting up this study and to all the assistants who collected data over the years of this study. We thank two anonymous reviewers whose comments helped clarify the results and improve the manuscript. NR 87 TC 1 Z9 1 U1 6 U2 59 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD APR PY 2014 VL 44 IS 4 BP 350 EP 364 DI 10.1139/cjfr-2013-0375 PG 15 WC Forestry SC Forestry GA AD7OI UT WOS:000333453100008 ER PT J AU Hadden, JA French, AD Woods, RJ AF Hadden, Jodi A. French, Alfred D. Woods, Robert J. TI Effect of microfibril twisting on theoretical powder diffraction patterns of cellulose I beta SO CELLULOSE LA English DT Article DE Cellulose; Microfibril twist; Molecular dynamics; X-ray diffraction ID MOLECULAR-DYNAMICS; SIMULATION; GROMACS; FIBER AB Previous studies of calculated diffraction patterns for cellulose crystallites suggest that distortions that arise once models have been subjected to molecular dynamics (MD) simulation are the result of both microfibril twisting and changes in unit cell dimensions induced by the empirical force field; to date, it has not been possible to separate the individual contributions of these effects. To provide a better understanding of how twisting manifests in diffraction data, the present study demonstrates a method for generating twisted and linear cellulose structures that can be compared without the bias of dimensional changes, allowing assessment of the impact of twisting alone. Analysis of unit cell dimensions, microfibril volume, hydrogen bond patterns, glycosidic torsion angles, and hydroxymethyl group orientations confirmed that the twisted and linear structures collected with this method were internally consistent, and theoretical powder diffraction patterns for the two were shown to be effectively indistinguishable. These results indicate that differences between calculated patterns for the crystal coordinates and twisted structures from MD simulation can result entirely from changes in unit cell dimensions, and not from microfibril twisting. Although powder diffraction patterns for models in the 81-chain size regime were shown to be unaffected by twisting, suggesting that a modest degree of twist is not inconsistent with available crystallographic data, it may be that other diffraction techniques are capable of detecting this structural difference. Until such time as definitive experimental evidence comes to light, the results of this study suggest that both twisted and linear microfibrils may represent an appropriate model for cellulose I beta. C1 [Hadden, Jodi A.; Woods, Robert J.] Univ Georgia, Complex Carbohydrate Ctr, Athens, GA 30602 USA. [French, Alfred D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Woods, Robert J.] Natl Univ Ireland, Sch Chem, Galway, Ireland. RP Woods, RJ (reprint author), Univ Georgia, Complex Carbohydrate Ctr, 315 Riverbend Rd, Athens, GA 30602 USA. EM rwoods@ccrc.uga.edu RI Woods, Robert/F-4962-2015 FU Cotton, Inc.; National Institutes for Health [GM094919]; Science Foundation of Ireland [08/IN.1/B2070] FX The authors are grateful to Cotton, Inc. for support of this work, as well as the National Institutes for Health (GM094919 (EUREKA)) and the Science Foundation of Ireland (08/IN.1/B2070). NR 25 TC 8 Z9 8 U1 1 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2014 VL 21 IS 2 SI SI BP 879 EP 884 DI 10.1007/s10570-013-0051-z PG 6 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AD4IW UT WOS:000333213800004 PM 24729665 ER PT J AU French, AD AF French, Alfred D. TI Idealized powder diffraction patterns for cellulose polymorphs SO CELLULOSE LA English DT Article DE Cellulose crystal structure; Miller indices; Powder diffraction patterns; Convention ID NEUTRON FIBER DIFFRACTION; SYNCHROTRON X-RAY; HYDROGEN-BONDING SYSTEM; CRYSTAL-STRUCTURE; PACKING ANALYSIS; POLYSACCHARIDES; MODEL AB Cellulose samples are routinely analyzed by X-ray diffraction to determine their crystal type (polymorph) and crystallinity. However, the connection is seldom made between those efforts and the crystal structures of cellulose that have been proposed with synchrotron X-radiation and neutron diffraction over the past decade or so. In part, this desirable connection is thwarted by the use of different conventions for description of the unit cells of the crystal structures. In the present work, powder diffraction patterns from cellulose I alpha, I beta, II, IIII, and IIIII were calculated based on the published atomic coordinates and unit cell dimensions contained in modified "crystal information files" (.cif) that are supplied in the Supplementary Information. The calculations used peak widths at half maximum height of both 0.1 and 1.5A degrees 2 theta, providing both highly resolved indications of the contributions of each contributing reflection to the observable diffraction peaks as well as intensity profiles that more closely resemble those from practical cellulose samples. Miller indices are shown for each contributing peak that conform to the convention with c as the fiber axis, a right-handed relationship among the axes and the length of a < b. Adoption of this convention, already used for crystal structure determinations, is also urged for routine studies of polymorph and crystallinity. The calculated patterns are shown with and without preferred orientation along the fiber axis. Diffraction intensities, output by the Mercury program from the Cambridge Crystallographic Data Centre, have several uses including comparisons with experimental data. Calculated intensities from different polymorphs can be added in varying proportions using a spreadsheet program to simulate patterns such as those from partially mercerized cellulose or various composites. C1 USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP French, AD (reprint author), USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM cellulose.editor@gmail.com NR 22 TC 203 Z9 203 U1 15 U2 112 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2014 VL 21 IS 2 SI SI BP 885 EP 896 DI 10.1007/s10570-013-0030-4 PG 12 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AD4IW UT WOS:000333213800005 ER PT J AU French, AD Concha, M Dowd, MK Stevens, ED AF French, Alfred D. Concha, Monica Dowd, Michael K. Stevens, Edwin D. TI Electron (charge) density studies of cellulose models SO CELLULOSE LA English DT Article DE Electron density topology; Non-covalent bonding; Carbohydrate; Model of cellulose; Hydrogen bonding; Atoms-in-Molecules ID NEUTRON FIBER DIFFRACTION; ATOMIC-FORCE MICROSCOPY; HYDROGEN-BOND STRENGTHS; SYNCHROTRON X-RAY; CRYSTAL-STRUCTURE; I-BETA; CELLOBIOSE; INSIGHTS AB Introductory material first describes electron density approaches and demonstrates visualization of electron lone pairs and bonding as concentrations of electron density. Then it focuses on the application of Bader's Quantum Theory of Atoms-in-Molecules (AIM) to cellulose models. The purpose of the work is to identify the various interactions that stabilize cellulose structure. AIM analysis aids study of non-covalent interactions, especially those for which geometric criteria are not well established. The models were in the form of pairs of cellotriose molecules, methylated at the O1 and O4 ends. Based on the unit cell of cellulose I beta, there were corner-corner, and center-center pairs that correspond to (200) sheets, and corner-center pairings that corresponded to (1-10) and (110) stacks. AIM analysis (or charge-density topology analysis) was applied before and after minimization in vacuum and in continuum solvation. Besides the conventional O-H center dot center dot center dot O hydrogen bonds, all of which were known from geometric criteria, C-H center dot center dot center dot O hydrogen bonds (some previously reported), and some O center dot center dot center dot O and H center dot center dot center dot H interactions were found. Non-covalent bonds in the (200) sheets were maintained in all calculations with the exception of a weak, bifurcated O6-H center dot center dot center dot O2'' bond that was not found in the corner-corner pair model and did not survive minimization. Nor did the O6 center dot center dot center dot O4 interactions on the reducing ends of the triosides. Pairs of molecules along the (110) plane had an equal number (12) of non-covalent bonds compared to the pairs along the (1-10) plane, but the AIM parameters indicated the bonds between the pairs in the (110) plane were weaker. Intra-molecular O-H center dot center dot center dot O hydrogen bonds survived in these minimized pairs, but the relative chain alignments usually did not. C1 [French, Alfred D.; Concha, Monica; Dowd, Michael K.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [French, Alfred D.; Stevens, Edwin D.] Univ New Orleans, Dept Chem, New Orleans, LA 70148 USA. RP French, AD (reprint author), USDA, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM al.french@ars.usda.gov NR 31 TC 20 Z9 20 U1 4 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD APR PY 2014 VL 21 IS 2 SI SI BP 1051 EP 1063 DI 10.1007/s10570-013-0042-0 PG 13 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AD4IW UT WOS:000333213800018 ER PT J AU Maron, JL Auge, H Pearson, DE Korell, L Hensen, I Suding, KN Stein, C AF Maron, John L. Auge, Harald Pearson, Dean E. Korell, Lotte Hensen, Isabell Suding, Katharine N. Stein, Claudia TI Staged invasions across disparate grasslands: effects of seed provenance, consumers and disturbance on productivity and species richness SO ECOLOGY LETTERS LA English DT Article DE small mammals; species richness; grasslands; exotic species; local filters; plant competition; Community assembly; plant productivity; invasion ID ENEMY RELEASE; BIOTIC RESISTANCE; PLANT INVASIONS; CONCEPTUAL-FRAMEWORK; HOT-SPOTS; DIVERSITY; METAANALYSIS; PREDATION; COMMUNITY; INVADERS AB Exotic plant invasions are thought to alter productivity and species richness, yet these patterns are typically correlative. Few studies have experimentally invaded sites and asked how addition of novel species influences ecosystem function and community structure and examined the role of competitors and/or consumers in mediating these patterns. We invaded disturbed and undisturbed subplots in and out of rodent exclosures with seeds of native or exotic species in grasslands in Montana, California and Germany. Seed addition enhanced aboveground biomass and species richness compared with no-seeds-added controls, with exotics having disproportionate effects on productivity compared with natives. Disturbance enhanced the effects of seed addition on productivity and species richness, whereas rodents reduced productivity, but only in Germany and California. Our results demonstrate that experimental introduction of novel species can alter ecosystem function and community structure, but that local filters such as competition and herbivory influence the magnitude of these impacts. C1 [Maron, John L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Auge, Harald; Korell, Lotte] UFZ, Dept Community Ecol, Helmholtz Ctr Environm Res, D-06120 Halle, Germany. [Auge, Harald; Hensen, Isabell] German Ctr Integrat Biodivers Res iDiv, D-4103 Leipzig, Germany. [Pearson, Dean E.] USDA Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Korell, Lotte; Hensen, Isabell] Univ Halle Wittenberg, Inst Biol, D-06108 Halle, Germany. [Suding, Katharine N.; Stein, Claudia] Univ Calif Berkeley, Berkeley, CA 94720 USA. RP Maron, JL (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. EM john.maron@mso.umt.edu RI Auge, Harald/D-4802-2015 OI Auge, Harald/0000-0001-7432-8453 FU USDA Cooperative State Research, Education and Extension Service [2005-35101-16040, 2006-01350]; NSF [DEB-0614406, DEB-1001807]; German Academic Exchange Service [DAAD 50750649]; Graduate School HIGRADE FX We thank the many people who helped with field/lab assistance in Montana, Germany and California, who are too numerous to mention individually here. We greatly appreciate the Montana Department of Fish Wildlife and Parks, the U.S. Fish and Wildlife Service, Pepperwood Preserve, the University of Montana and numerous private land owners in Montana and Germany for allowing us to work on their lands. This research was supported with grants from the USDA Cooperative State Research, Education and Extension Service (2005-35101-16040 to JLM/DEP, 2006-01350 to KS), NSF (DEB-0614406 to JLM/DEP, DEB-1001807 to KNS), the German Academic Exchange Service (DAAD 50750649) to HA, the Graduate School HIGRADE to LK and by generous travel support from the Global Invasions Network NSF (RCN DEB-0541673; PIs R. Hufbauer and M. Torchin). NR 46 TC 13 Z9 13 U1 4 U2 101 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-023X EI 1461-0248 J9 ECOL LETT JI Ecol. Lett. PD APR PY 2014 VL 17 IS 4 BP 499 EP 507 DI 10.1111/ele.12250 PG 9 WC Ecology SC Environmental Sciences & Ecology GA AC0SZ UT WOS:000332206400011 PM 24467348 ER PT J AU Pitt, WC Witmer, GW Jojola, SM Sin, H AF Pitt, William C. Witmer, Gary W. Jojola, Susan M. Sin, Hans TI Potential citric acid exposure and toxicity to Hawaiian hoary bats (Lasiurus cinereus semotus) associated with Eleutherodactylus frog control SO ECOTOXICOLOGY LA English DT Article DE Hoary bat; Lasiurus cinereus semotus; Coqui frog; Eleutherodactylus coqui; Control; Risk; Citric acid; Hawaii AB We examined potential exposure of Hawaiian hoary bats (Lasiurus cinereus semotus) to citric acid, a minimum risk pesticide registered for control of invasive Eleutherodactylus frog populations. Hoary bats are nocturnal insectivores that roost solitarily in foliage, federally listed as endangered, and are endemic to Hawaii. Oral ingestion during grooming of contaminated fur appears to be the principal route by which these bats might be exposed to citric acid. We made assessments of oral toxicity, citric acid consumption, retention of material on fur, and grooming using big brown bats (Eptesicus fuscus) as a surrogate species. We evaluated both ground application and aerial application of 16 % solutions of citric acid during frog control operations. Absorbent bat effigies exposed to ground and aerial operational spray applications retained means of 1.54 and 0.02 g, respectively, of dry citric acid, although retention by the effigies was much higher than bat carcasses drenched in citric acid solutions. A high dose delivered orally (2,811 mg/kg) was toxic to the big brown bats and emesis occurred in 1 bat dosed as low as the 759 mg/kg level. No effect was observed with the lower doses examined (a parts per thousand currency sign542 mg/kg). Bats sprayed with 5 ml of 16 % (w/w) citric acid solution showed no evidence of intoxication. In field situations, it is unlikely that bats would be sprayed directly or ingest much citric acid retained by fur. Based on our observations, we believe Hawaiian hoary bats to be at very low risk from harmful exposure to a toxic dose of citric acid during frog control operations. C1 [Pitt, William C.] US Anim & Plant Hlth Inspect Serv, Hawaii Field Stn, Natl Wildlife Res Ctr, Wildlife Serv,USDA, Hilo, HI 96721 USA. [Witmer, Gary W.] US Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, Wildlife Serv, USDA, Ft Collins, CO 80521 USA. [Jojola, Susan M.] Natl Wildlife Res Ctr, Ft Collins, CO USA. [Sin, Hans] Natl Wildlife Res Ctr, Hilo, HI USA. RP Pitt, WC (reprint author), US Anim & Plant Hlth Inspect Serv, Hawaii Field Stn, Natl Wildlife Res Ctr, Wildlife Serv,USDA, POB 10880, Hilo, HI 96721 USA. EM will.pitt@aphis.usda.gov FU State of Hawaii Department of Agriculture under APHIS [07-7415-0639] FX We thank Patrick Burke for assistance with captive bat experimentation and Roberta Swift and Michelle Higashi for field assistance during the effigy trials. We appreciate the assistance of Dr. Tom O'Shea (US Geologic Survey) and his staff in capturing big brown bats for the study and for suggestions for the care of bats in captivity. Dr. Mary Mendonca, Auburn University, provided advice on captive bat care and feeding and Dr. Richard Bowen, Colorado State University, loaned us some of the bat cages used. The State of Hawaii Department of Agriculture sponsored the laboratory portion of this under APHIS Cooperative Agreement #07-7415-0639 (RA), as QA 1403, approved by the National Wildlife Research Center Institutional Animal Care and Use Committee; field studies were conducted as QA 1331. We appreciate Joy Browning and two anonymous reviewers for providing editorial suggestions. We thank Michael W. Fall for assistance with literature review and manuscript preparation. NR 28 TC 1 Z9 1 U1 6 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0963-9292 EI 1573-3017 J9 ECOTOXICOLOGY JI Ecotoxicology PD APR PY 2014 VL 23 IS 3 BP 429 EP 436 DI 10.1007/s10646-014-1208-8 PG 8 WC Ecology; Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA AD3GZ UT WOS:000333127600011 PM 24526591 ER PT J AU Pojednic, RM Ceglia, L AF Pojednic, Rachele M. Ceglia, Lisa TI The Emerging Biomolecular Role of Vitamin D in Skeletal Muscle SO EXERCISE AND SPORT SCIENCES REVIEWS LA English DT Review DE skeletal muscle; 25-hydroxyvitamin D; aging; vitamin D receptor; vitamin D ID D-RECEPTOR GENE; RANDOMIZED CONTROLLED-TRIAL; KAPPA-B ACTIVITY; 1-ALPHA,25-DIHYDROXYVITAMIN D-3; 1,25-DIHYDROXYVITAMIN D-3; MYOGENIC DIFFERENTIATION; CALCIUM SUPPLEMENTATION; 25-HYDROXYVITAMIN D; GROWTH-FACTORS; CELLS AB In this review, we summarize current evidence for a direct effect of vitamin D on skeletal muscle. A number of studies identify the receptor for 1,25-dihydroxyvitamin-D-3 (vitamin D receptor (VDR)) and the enzyme CYP27B1 (1-alpha-hydroxylase) in muscle. We hypothesize that vitamin D acts on myocytes via the VDR, and we examine proposed effects on myocyte proliferation, differentiation, growth, and inflammation. C1 [Pojednic, Rachele M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA. [Ceglia, Lisa] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA USA. [Ceglia, Lisa] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Bone Metab Lab, Boston, MA 02111 USA. RP Pojednic, RM (reprint author), Tufts Univ, Jean Mayer USDA HNRCA, Nutr Exercise Physiol & Sarcopenia Lab, 711 Washington St, Boston, MA 02111 USA. EM rachele.pojednic@tufts.edu FU NHLBI [T32HL69772] FX The manuscript preparation was supported by funding from an NHLBI pre-doctoral training grant (T32HL69772) to R.M. Pojednic. NR 48 TC 16 Z9 16 U1 0 U2 13 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0091-6331 EI 1538-3008 J9 EXERC SPORT SCI REV JI Exerc. Sport Sci. Rev. PD APR PY 2014 VL 42 IS 2 BP 76 EP 81 DI 10.1249/JES.0000000000000013 PG 6 WC Physiology; Sport Sciences SC Physiology; Sport Sciences GA AD3TU UT WOS:000333169900006 PM 24508736 ER PT J AU Panaccione, DG Beaulieu, WT Cook, D AF Panaccione, Daniel G. Beaulieu, Wesley T. Cook, Daniel TI Bioactive alkaloids in vertically transmitted fungal endophytes SO FUNCTIONAL ECOLOGY LA English DT Article DE defensive mutualism; ergot alkaloids; indole-diterpenes; lolines; peramine; plant-herbivore interactions; swainsonine; symbiosis ID IPOMOEA-ASARIFOLIA CONVOLVULACEAE; ALPHA-MANNOSIDASE INHIBITOR; INFECTED PERENNIAL RYEGRASS; LYSOSOMAL STORAGE DISEASE; GENE-KNOCKOUT ENDOPHYTES; TALL FESCUE TOXICOSIS; ERGOT ALKALOIDS; NEOTYPHODIUM-LOLII; GRASS-ENDOPHYTE; LOLITREM-B AB 1. Plants form mutualistic symbioses with a variety of microorganisms including endophytic fungi that live inside the plant and cause no overt symptoms of infection. Some endophytic fungi form defensive mutualisms based on the production of bioactive metabolites that protect the plant from herbivores in exchange for a protected niche and nutrition from the host plant. Key elements of these symbioses are vertical transmission of the fungus through seed of the host plant, a narrow host range, and production of bioactive metabolites by the fungus. 2. Grasses frequently form symbioses with endophytic fungi belonging to the family Clavicipitaceae. These symbioses have been studied extensively because of their significant impacts on insect and mammalian herbivores. Many of the impacts are likely due to the production of four classes of bioactive alkaloids - ergot alkaloids, lolines, indole-diterpenes and peramine-that are distributed in different combinations among endophyte taxa. 3. Several legumes, including locoweeds, are associated with a toxic syndrome called locoism as a result of their accumulation of swainsonine. Species in two genera were recently found to contain previously undescribed endophytic fungi (Undifilum spp., family Pleosporaceae) that are the source of that toxin. The fungi are strictly vertically transmitted and have narrow host ranges. 4. Some plant species in the morning glory family (Convolvulaceae) also form symbioses with endophytic fungi of the Clavicipitaceae that produce ergot alkaloids and, perhaps in at least one case, lolines. Other species in this plant family form symbioses with undescribed fungi that produce swainsonine. The swainsonine-producing endophytes associated with the Convolvulaceae are distinct from the Undifilum spp. associated with locoweeds and the Clavicipitaceous fungi associated with Convolvulaceae. 5. In the establishment of vertically transmitted symbioses, fungi must have entered the symbiosis with traits that were immediately useful to the plant. Bioactive metabolites are likely candidates for such pre-adapted traits which were likely useful to the free-living fungi as well. With future research, vertically transmitted fungi from diverse clades with narrow host ranges and that produce bioactive compounds are likely to be found as important mutualists in additional plants. C1 [Panaccione, Daniel G.] W Virginia Univ, Div Plant & Soil Sci, Morgantown, WV 26506 USA. [Beaulieu, Wesley T.] Indiana Univ, Dept Biol, Bloomington, IN USA. [Cook, Daniel] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84321 USA. RP Panaccione, DG (reprint author), W Virginia Univ, Div Plant & Soil Sci, 1090 Agr Sci Bldg, Morgantown, WV 26506 USA. EM danpan@wvu.edu FU U.S. Department of Agriculture National Institute of Food and Agriculture [2012-67013-19384] FX Funding from the U.S. Department of Agriculture National Institute of Food and Agriculture (2012-67013-19384) to D.G.P. is gratefully acknowledged. We thank Keith Clay for helpful guidance on the content of this review, Christopher Schardl and an anonymous reviewer for constructive comments on a previous version of this article, and Sarah Robinson for assistance with the bibliography. This article is published with permission of the West Virginia Agriculture and Forestry Experiment Station as scientific article number 3155. NR 155 TC 24 Z9 25 U1 4 U2 87 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-8463 EI 1365-2435 J9 FUNCT ECOL JI Funct. Ecol. PD APR PY 2014 VL 28 IS 2 SI SI BP 299 EP 314 PG 16 WC Ecology SC Environmental Sciences & Ecology GA AC8IU UT WOS:000332777500003 ER PT J AU Sukumar, N Nicholson, TL Conover, MS Ganguly, T Deora, R AF Sukumar, Neelima Nicholson, Tracy L. Conover, Matt S. Ganguly, Tridib Deora, Rajendar TI Comparative Analyses of a Cystic Fibrosis Isolate of Bordetella bronchiseptica Reveal Differences in Important Pathogenic Phenotypes SO INFECTION AND IMMUNITY LA English DT Article ID BIOFILM FORMATION; RESPIRATORY-TRACT; TRACHEAL COLONIZATION; INFECTIOUS-DISEASES; SIGNAL-TRANSDUCTION; PERTUSSIS; IDENTIFICATION; DIVERSITY; REGULON; BVGAS AB Bordetella bronchiseptica is a Gram-negative bacterium that infects and causes disease in a wide variety of animals. B. bronchiseptica also infects humans, thereby demonstrating zoonotic transmission. An extensive characterization of human B. bronchiseptica isolates is needed to better understand the distinct genetic and phenotypic traits associated with these zoonotic transmission events. Using whole-genome transcriptome and CGH analysis, we report that a B. bronchiseptica cystic fibrosis isolate, T44625, contains a distinct genomic content of virulence-associated genes and differentially expresses these genes compared to the sequenced model laboratory strain RB50, a rabbit isolate. The differential gene expression pattern correlated with unique phenotypes exhibited by T44625, which included lower motility, increased aggregation, hyperbiofilm formation, and an increased in vitro capacity to adhere to respiratory epithelial cells. Using a mouse intranasal infection model, we found that although defective in establishing high bacterial burdens early during the infection process, T44625 persisted efficiently in the mouse nose. By documenting the unique genomic and phenotypic attributes of T44625, this report provides a blueprint for understanding the successful zoonotic potential of B. bronchiseptica and other zoonotic bacteria. C1 [Sukumar, Neelima; Conover, Matt S.; Ganguly, Tridib; Deora, Rajendar] Wake Forest Univ Hlth Sci, Dept Microbiol & Immunol, Winston Salem, NC 27157 USA. [Nicholson, Tracy L.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Deora, R (reprint author), Wake Forest Univ Hlth Sci, Dept Microbiol & Immunol, Winston Salem, NC 27157 USA. EM rdeora@wakehealth.edu FU NIH [1R01AI075081] FX Research in the laboratory of R.D. is supported by funds from the NIH (grant no. 1R01AI075081). NR 65 TC 2 Z9 2 U1 0 U2 4 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0019-9567 EI 1098-5522 J9 INFECT IMMUN JI Infect. Immun. PD APR PY 2014 VL 82 IS 4 BP 1627 EP 1637 DI 10.1128/IAI.01453-13 PG 11 WC Immunology; Infectious Diseases SC Immunology; Infectious Diseases GA AD4BX UT WOS:000333192300029 PM 24470470 ER PT J AU Brynildsrud, O Feil, EJ Bohlin, J Castillo-Ramirez, S Colquhoun, D McCarthy, U Matejusova, IM Rhodes, LD Wiens, GD Verner-Jeffreys, DW AF Brynildsrud, Ola Feil, Edward J. Bohlin, Jon Castillo-Ramirez, Santiago Colquhoun, Duncan McCarthy, Una Matejusova, Iveta M. Rhodes, Linda D. Wiens, Gregory D. Verner-Jeffreys, David W. TI Microevolution of Renibacterium salmoninarum: evidence for intercontinental dissemination associated with fish movements SO ISME JOURNAL LA English DT Article DE fish pathogens; microbial evolution; next-generation sequencing; Renibacterium salmoninarum; transmission history ID BACTERIAL KIDNEY-DISEASE; PHYLOGENETIC ANALYSIS; ANTIGENIC VARIATION; CAUSATIVE AGENT; PACIFIC SALMON; SEQUENCE; TROUT; EVOLUTION; PATHOGEN; TRANSMISSION AB Renibacterium salmoninarum is the causative agent of bacterial kidney disease, a major pathogen of salmonid fish species worldwide. Very low levels of intra-species genetic diversity have hampered efforts to understand the transmission dynamics and recent evolutionary history of this Gram-positive bacterium. We exploited recent advances in the next-generation sequencing technology to generate genome-wide single-nucleotide polymorphism (SNP) data from 68 diverse R. salmoninarum isolates representing broad geographical and temporal ranges and different host species. Phylogenetic analysis robustly delineated two lineages (lineage 1 and lineage 2); futhermore, dating analysis estimated that the time to the most recent ancestor of all the isolates is 1239 years ago (95% credible interval (CI) 444-2720 years ago). Our data reveal the intercontinental spread of lineage 1 over the last century, concurrent with anthropogenic movement of live fish, feed and ova for aquaculture purposes and stocking of recreational fisheries, whilst lineage 2 appears to have been endemic in wild Eastern Atlantic salmonid stocks before commercial activity. The high resolution of the SNP-based analyses allowed us to separate closely related isolates linked to neighboring fish farms, indicating that they formed part of single outbreaks. We were able to demonstrate that the main lineage 1 subgroup of R. salmoninarum isolated from Norway and the UK likely represent an introduction to these areas similar to 40 years ago. This study demonstrates the promise of this technology for analysis of micro and medium scale evolutionary relationships in veterinary and environmental microorganisms, as well as human pathogens. C1 [Brynildsrud, Ola; Bohlin, Jon] Norwegian Sch Vet Sci, Dept Food Safety & Infect Biol, EpiCtr, Oslo, Norway. [Feil, Edward J.; Castillo-Ramirez, Santiago] Univ Bath, Dept Biol & Biochem, Bath BA2 7AY, Avon, England. [Colquhoun, Duncan] Norwegian Vet Inst, Dept Lab Serv, Bacteriol Sect, Oslo, Norway. [McCarthy, Una; Matejusova, Iveta M.] Marine Scotland Sci, Aberdeen, Scotland. [Rhodes, Linda D.] NOAA, NW Fisheries Sci Ctr, Seattle, WA USA. [Wiens, Gregory D.] USDA, Natl Ctr Cool & Coldwater Aquaculture, Leetown, WV USA. [Verner-Jeffreys, David W.] The Nothe, Cefas Weymouth Lab, Weymouth, Dorset, England. RP Verner-Jeffreys, DW (reprint author), CEFAS, Cefas Weymouth Lab, Barrack Rd, Weymouth DT4 8UB, Dorset, England. EM david.verner-jeffreys@cefas.co.uk OI Wiens, Gregory/0000-0002-5478-7780; Brynildsrud, Ola/0000-0001-7566-4133; Bohlin, Jon/0000-0002-0992-1311; Rhodes, Linda/0000-0003-4995-9426 FU UK's Department for Environment Food and Rural Affairs [FB002, DP258] FX The UK's Department for Environment Food and Rural Affairs provided funding through Projects FB002 and an interagency grant DP258. Joel Caren, Inger Lise Engen and Georgina Rimmer provided valuable technical support. We thank Dr Bill Keleher for providing isolates from New Brunswick, Canada. Dr Chris Watkins from The Genome Analysis Centre (TGAC), Norwich, UK provided expert assistance with sequencing the isolates. We are grateful to the reviewers for their helpful comments. NR 63 TC 5 Z9 5 U1 3 U2 19 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 EI 1751-7370 J9 ISME J JI ISME J. PD APR PY 2014 VL 8 IS 4 BP 746 EP 756 DI 10.1038/ismej.2013.186 PG 11 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA AD4BA UT WOS:000333189700002 PM 24173459 ER PT J AU French, BW Coates, BS Sappington, TW AF French, B. W. Coates, B. S. Sappington, T. W. TI Inheritance of an extended diapause trait in the Northern corn rootworm, Diabrotica barberi (Coleoptera: Chrysomelidae) SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article ID PROLONGED DIAPAUSE; STOCHASTIC ENVIRONMENTS; LEPIDOPTERA-NOCTUIDAE; VIRGIFERA COLEOPTERA; VARIABLE ENVIRONMENT; SOIL INSECTICIDES; TRANSGENIC CORN; PRAIRIE GRASSES; BOMBYX-MORI; WESTERN C1 [French, B. W.] USDA ARS, North Cent Agr Res Lab, Brookings, SD USA. [Coates, B. S.; Sappington, T. W.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA USA. RP French, BW (reprint author), USDA ARS, NCARL, 2923 Medary Ave, Brookings, SD 57006 USA. EM wade.french@ars.usda.gov NR 87 TC 4 Z9 4 U1 4 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD APR PY 2014 VL 138 IS 3 BP 213 EP 221 PG 9 WC Entomology SC Entomology GA AC8RN UT WOS:000332801500007 ER PT J AU Baldwin, RL Zhang, A Fultz, SW Abubeker, S Harris, C Connor, EE Van Hekken, DL AF Baldwin, R. L. Zhang, A. Fultz, S. W. Abubeker, S. Harris, C. Connor, E. E. Van Hekken, D. L. TI Hot topic: Brown marmorated stink bug odor compounds do not transfer into milk by feeding bug-contaminated corn silage to lactating dairy cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE dairy cow; brown marmorated stink bug; milk; stink bug odor ID VOLATILE FLAVOR COMPOUNDS; SEX-PHEROMONE; IDENTIFICATION; AROMA; PENTATOMIDAE; COMPONENTS; CHEESE; LIFE; MASS AB Brown marmorated stink bug (BMSB; Halyomorpha halys) is an emerging invasive species of grave concern to agriculture as a polyphagous plant pest with potential negative effects on the dairy industry. The purpose of this study was to determine the risk of including BMSB-contaminated silage in lactating dairy cow rations. First, 6 dairies, either highly infested (n = 3; 30 to 100 bugs per stalk) or not infested (n = 3), were sampled to assess the prevalence of bug secretion compounds tridecane (major component) and E-2-decenal (stink odor component) in silage and milk. Second, using wild BMSB, a mini-silo dose-response experiment (adding 100, 50, 25, 10, and 1 freshly crushed bugs/0.5 kg of chopped corn) was conducted to assess the effect of ensiling on BMSB stink odor compounds. Finally, synthetic BMSB stink odor compounds (10 g of tridecane and 5 g of E-2-decenal) were ruminally infused twice daily over 3 d, and samples of milk, urine, and rumen fluid were collected to evaluate disposition. Bug stink odor compounds were sampled by solid-phase microextraction (SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Milk production and feed composition were unaffected when BMSB-contaminated silage was fed. Moreover, no E-2-decenal was detected in silage or milk (detection threshold = 0.00125 mu g/mL). The dose-response of tridecane in mini-silo samples exhibited a linear relationship (R-2 = 0.78) with the amount of BMSB added; however, E-2-decenal was completely decomposed and undetectable in spiked mini-silos after ensiling. Both synthetic secretion compounds infused into rumen were undetectable in all milk and urine samples. E-2-Decenal was not detectable in rumen fluid, whereas tridecane was detected only at 15 min postinfusion but not present thereafter. Feed intake was unaffected by infusion treatment and BMSB secretion compounds (E-2-decenal and tridecane) were not observed in milk. E-2-Decenal and tridecane from the metathoracic gland of BMSB are not able to contaminate milk either due to the ensiling process or because of metabolism within the rumen. Concern over BMSB stink odor compounds contaminating the fluid milk supply, even on highly infested farms, is not warranted. C1 [Baldwin, R. L.; Connor, E. E.] ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD 20705 USA. [Zhang, A.; Abubeker, S.; Harris, C.] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. [Fultz, S. W.] Univ Maryland UMD Extens, Ft Detrick, MD 21702 USA. [Van Hekken, D. L.] ARS, Dairy & Funct Foods Res Unit, USDA, Wyndmoor, PA 19038 USA. RP Baldwin, RL (reprint author), ARS, Bovine Funct Genom Lab, USDA, Beltsville, MD 20705 USA. EM ransom.baldwin@ars.usda.gov NR 25 TC 4 Z9 4 U1 3 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD APR PY 2014 VL 97 IS 4 BP 1877 EP 1884 DI 10.3168/jds.2013-7545 PG 8 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AD3KM UT WOS:000333138000002 PM 24565323 ER PT J AU Yao, C Weigel, KA Cole, JB AF Yao, C. Weigel, K. A. Cole, J. B. TI Short communication: Genetic evaluation of stillbirth in US Brown Swiss and Jersey cattle SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE genetic evaluation; stillbirth; Brown Swiss; Jersey ID GRANDSIRE THRESHOLD-MODEL; UNITED-STATES; CALVING EASE; BIRTH-WEIGHT; GESTATION LENGTH; PERINATAL-MORTALITY; CONCEPTION RATE; HOLSTEIN SIRES; DAIRY-CATTLE; DYSTOCIA AB Stillbirth (SB) often results in reduced milk yield, compromised reproductive performance, and decreased dam longevity. Corrective mating can be used as a short-term solution to the problem, but long-term improvement of the population requires the routine calculation of genetic evaluations. Breeding values for SB have been available for Holstein (HO) bulls since 2006, but not for Brown Swiss (BS) or Jersey (JE) bulls. In this study, a multi-breed sire-maternal grandsire threshold model was used to perform genetic evaluations for SB of BS, JE, and HO bulls using more than 14 million purebred and crossbred calving records. Phenotypically, the percentage of SB (%SB) across all lactations were 3.7% in JE, 5.1% in BS, and 6.3% in HO. Direct heritabilities for BS, JE, and HO were 0.008, 0.007, and 0.008, and maternal heritabilities were 0.002, 0.016, and 0.021, respectively. Compared with HO, crossbred calvings from BS and JE bulls bred to HO cows lowered %SB by 1.5 and 1.2%, respectively. In general, %SB increased considerably as calving difficulty increased in all 3 breeds; however, in JE, %SB was constant for dystocia scores of 3 (needed assistance), 4 (considerable force), and 5 (extreme difficulty). Compared with purebred HO calvings, purebred BS and JE calvings had lower phenotypic %SB by up to 5.5 and 7.8%, respectively, and BS x HO and JE x HO crossbred calvings decreased %SB by up to 3.8 and 4.1%, respectively. As expected, SB rates in primiparous cows were higher than those in multiparous cows. Female calves had greater %SB than male calves in all parities for JE and in second-and-later parities for BS. Favorable (decreasing) phenotypic and genetic trends from 1999 to 2009 were observed in all 3 breeds. Heterosis of SB for BS and JE was -0.026 and -0.149, respectively, on the underlying scale, which corresponds to effects on service-sire SB (SSB) and daughter SB (DSB) predicted transmitting ability (PTA) of -0.3 and -0.5% in BS, and -1.5 and -2.7% in JE. Overall, in the current population, BS bulls had the most desirable average SSB PTA of 4.8%, compared with 5.6% for JE and 5.5% for HO. Brown Swiss and JE bulls both had average DSB PTA of 6.5%, lower than that of 7.7% in HO. Average reliabilities of SSB and DSB in 3 breeds ranged from 45 to 50%. The use of a BS-JE-HO multibreed genetic evaluation for SB in the United States is feasible, and the addition of SSB and DSB to the lifetime net merit selection index will help improve the profitability of BS and JE cattle in the United States. C1 [Yao, C.; Weigel, K. A.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Cole, J. B.] ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. RP Cole, JB (reprint author), ARS, Anim Improvement Programs Lab, USDA, Beltsville, MD 20705 USA. EM john.cole@ars.usda.gov RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU National Association of Animal Breeders (Columbia, MO); Hatch grant from the Wisconsin Agricultural Experiment Station [WIS01595]; [1265-31000-096-00] FX The assistance of Jay Megonigal and Daniel Null (both of the Animal Improvement Programs Laboratory, Agricultural Research Service, US Department of Agriculture, Beltsville, MD) with data extraction and preparation is gratefully noted. The BLUPF90 family of computer programs was provided by Ignacy Misztal and Shogo Tsuruta of the University of Georgia (Athens). The cooperation of the American Jersey Cattle Association (Reynoldsville, OH), Brown Swiss Association (Beloit, WI), and Holstein Association USA (Brattleboro, VT) in supplying pedigree data for registered cows and the dairy records processing centers [AgriTech Analytics (Visalia, CA), AgSource Cooperative Services (Verona, WI), Dairy Records Management Systems (Raleigh, NC, and Ames, IA), and DHI Computing Services (Provo, UT)] in supplying pedigree data for grade cows and calving event data is acknowledged. The National Association of Animal Breeders (Columbia, MO) contributed to funding the national calving traits evaluations and this research project. Cole was supported by appropriated project 1265-31000-096-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information." Support for Yao was provided by Hatch grant #WIS01595 from the Wisconsin Agricultural Experiment Station and the National Association of Animal Breeders (Columbia, MO). The authors appreciate the input of two anonymous peer reviewers, whose advice greatly improved the manuscript. NR 30 TC 4 Z9 4 U1 0 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD APR PY 2014 VL 97 IS 4 BP 2474 EP 2480 DI 10.3168/jds.2013-7320 PG 7 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AD3KM UT WOS:000333138000063 PM 24508434 ER PT J AU Abdel-Haleem, H Carter, TE Rufty, TW Boerma, HR Li, ZL AF Abdel-Haleem, Hussein Carter, Thomas E., Jr. Rufty, Thomas W. Boerma, H. Roger Li, Zenglu TI Quantitative trait loci controlling aluminum tolerance in soybean: candidate gene and single nucleotide polymorphism marker discovery SO MOLECULAR BREEDING LA English DT Article DE Aluminum toxicity; Candidate gene; Citrate synthase; QTL; Root extension; Soybean; Tap root ID DNA-METHYLATION; MELASTOMA-MALABATHRICUM; PLANT INTRODUCTION; DROUGHT TOLERANCE; CITRATE SYNTHASE; ORGANIC-ACIDS; PI 416937; RESISTANCE; EXUDATION; IDENTIFICATION AB Aluminum (Al) toxicity is an important abiotic stress that affects soybean production in acidic soils throughout the world. Development of Al-tolerant cultivars is an efficient and environmentally friendly solution to the problem. A previous report identified quantitative trait loci (QTL) for Al tolerance inherited from PI 416937, using restriction fragment length polymorphism markers, in a population of Young x PI 416937. The population was genotyped with 162 simple sequence repeats to enhance the power of QTL detection and enable the selection of candidate genes for functional marker development. Two QTL that explained 54 % of the phenotypic variation in root extension under Al stress conditions (HIAL) were refined on chromosomes (chr) Gm08 and Gm16. Three QTL located on chr Gm08, Gm16 and Gm19 explained 59 % of the phenotypic variation in root extension as a percent of control (PC). Two major QTL, designated qAL_HIAL_08 and qAL_PC_08, controlling HIAL and PC, respectively, were mapped to the same genomic region on chr Gm08 and inherited their favorable allele from PI 416937. These QTL explained 45 and 41 % of phenotypic variation in HIAL and PC, respectively. Six homologues for citrate synthase (CS) genes were found in the soybean genome sequence at chr Gm02, Gm08, Gm14, Gm15, and Gm18. Sixteen single nucleotide polymorphisms (SNPs) were identified in the CS homologue on chr Gm08. A SimpleProbe assay of Glyma08g42400-SNP was developed for the major QTL on chr Gm08. The SNPs identified from this region could be used for marker-assisted selection of Al tolerance. C1 [Abdel-Haleem, Hussein; Boerma, H. Roger; Li, Zenglu] Univ Georgia, Ctr Appl Genet Technol, Inst Plant Breeding Genet & Genom, Athens, GA 30602 USA. [Carter, Thomas E., Jr.] ARS, USDA, Raleigh, NC 27695 USA. [Carter, Thomas E., Jr.; Rufty, Thomas W.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Abdel-Haleem, H (reprint author), Univ Georgia, Ctr Appl Genet Technol, Inst Plant Breeding Genet & Genom, 111 Riverbend Rd, Athens, GA 30602 USA. EM hussein@uga.edu FU Georgia Agricultural Experiment Stations; United Soybean Board FX This research was supported by funds allocated to the Georgia Agricultural Experiment Stations and grants from the United Soybean Board. NR 61 TC 1 Z9 2 U1 8 U2 45 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD APR PY 2014 VL 33 IS 4 BP 851 EP 862 DI 10.1007/s11032-013-9999-5 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AD3UD UT WOS:000333170800009 ER PT J AU Pham, AT Bilyeu, K Chen, PY Boerma, HR Li, ZL AF Pham, Anh-Tung Bilyeu, Kristin Chen, Pengyin Boerma, H. Roger Li, Zenglu TI Characterization of the fan1 locus in soybean line A5 and development of molecular assays for high-throughput genotyping of FAD3 genes SO MOLECULAR BREEDING LA English DT Article DE FAD3; Low linolenic; TaqMan assay genotyping; Marker-assisted selection ID 1-PERCENT LINOLENIC ACID; CAENORHABDITIS-ELEGANS; SEED TRAITS; REGISTRATION; OIL; STABILITY; PHENOTYPE; DELETION; MUTANTS; ALLELE AB Soybean is one of the most important oil crops worldwide, and reducing the linolenic acid content of soybean oil will provide increased stability of the oil to consumers and limit the amount of trans fat in processed foods. The linolenic content in soybean seed is controlled by three fatty acid desaturase (FAD) three enzymes, FAD3A, B, and C. The soybean lines with 1 % linolenic acid content which are widely used in breeding for reduced linolenic acid in the USA have mutations in each of the three FAD genes derived from lines A5 (deletion of FAD3A), A26, and A23 (missense mutations in FAD3B and C, respectively). Although soybean line A5 has been released for 30 years, the extent and definition of the deletion of the FAD3A gene has not been characterized, which has prevented researchers from designing robust molecular markers for effective marker-assisted selection (MAS). Using a PCR-based genomic strategy, we have identified a 6.4-kbp deletion of the FAD3A gene in A5 and developed a TaqMan detection assay by targeting the deletion junction in A5, which could be used to distinguish the homozygotes and heterozygotes of the gene. In addition, based on mutant single nucleotide polymorphisms in FAD3B and FAD3C identified in A26 and A23, respectively, we have also developed TaqMan assays for high-throughput MAS. The TaqMan assays have proven to be a very effective platform for detecting the mutant FAD3 alleles and thus will greatly facilitate high-throughput MAS for development of soybean lines with reduced linolenic acid content. C1 [Pham, Anh-Tung; Boerma, H. Roger; Li, Zenglu] Univ Georgia, Ctr Appl Genet Technol, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Bilyeu, Kristin] ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. [Chen, Pengyin] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Boerma, H. Roger] Georgia Seed Dev Commiss, Athens, GA 30605 USA. RP Li, ZL (reprint author), Univ Georgia, Ctr Appl Genet Technol, Dept Crop & Soil Sci, Athens, GA 30602 USA. EM anhpham@uga.edu; zli@uga.edu FU United Soybean Board FX The authors wish to acknowledge the excellent technical assistance provided by Christine Cole, and two undergraduate helpers Jonathan Serrano and Bryan Grommersch. Funding for this research was provided by the United Soybean Board. NR 41 TC 4 Z9 5 U1 0 U2 7 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD APR PY 2014 VL 33 IS 4 BP 895 EP 907 DI 10.1007/s11032-013-0003-1 PG 13 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AD3UD UT WOS:000333170800012 ER PT J AU Pogue, MG AF Pogue, M. G. TI A Review of the Copitarsia decolora (Guen,e) (Lepidoptera: Noctuidae) Species Complex with the Description of a New Species from Chile and Argentina SO NEOTROPICAL ENTOMOLOGY LA English DT Review DE Cryptic species; morphology; agriculture; pests; distribution ID CUCULLIINAE AB Copitarsia gibberosa n. sp. is described from Chile and Argentina. Morphological characters are discussed to differentiate it from Copitarsia decolora (Guen,e), Copitarsia incommoda (Walker), and Copitarsia corruda (Pogue & Simmons). Copitarsia corruda has its status revised based on CO1 and morphology. Copitarsia paraturbata Castillo & Angulo is a new synonym of C. incommoda based on morphology. Copitarsia uncilata Burgos & Leiva is a new synonym of C. decolora based on morphology. A review of recent literature revealed a misunderstanding of the complex of species related to C. decolora, and these papers are evaluated and species are identified. Host plant utilization is discussed between C. decolora and C. corruda. Adults and male and female genitalia are illustrated to differentiate between the species in the C. decolora species complex. Keys to male and females based on genitalic morphology are given. Distribution maps of collected specimens are provided. C1 ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst, Washington, DC 20013 USA. RP Pogue, MG (reprint author), ARS, Systemat Entomol Lab, PSI, USDA,Smithsonian Inst, POB 37012, Washington, DC 20013 USA. EM michael.pogue@ars.usda.gov NR 13 TC 0 Z9 1 U1 1 U2 10 PU ENTOMOLOGICAL SOC BRASIL PI LONDRINA, PA CAIXA POSTAL 481, 86001-970 LONDRINA,, PR, BRAZIL SN 1519-566X EI 1678-8052 J9 NEOTROP ENTOMOL JI Neotrop. Entomol. PD APR PY 2014 VL 43 IS 2 BP 143 EP 153 DI 10.1007/s13744-013-0190-9 PG 11 WC Entomology SC Entomology GA AD3GI UT WOS:000333125900007 PM 27193521 ER PT J AU Wurzburger, N Miniat, CF AF Wurzburger, Nina Miniat, Chelcy Ford TI Drought enhances symbiotic dinitrogen fixation and competitive ability of a temperate forest tree SO OECOLOGIA LA English DT Article DE Climate change; Biogeochemistry; Hydraulic conductance; Plant physiology; Stomatal conductance ID CLIMATE-CHANGE VULNERABILITY; LOCUST ROBINIA-PSEUDOACACIA; NITROGEN-FIXATION; XYLEM CAVITATION; HYDRAULIC CONDUCTIVITY; WATER TRANSPORT; MORTALITY; PERFORMANCE; SEEDLINGS; EMBOLISM AB General circulation models project more intense and frequent droughts over the next century, but many questions remain about how terrestrial ecosystems will respond. Of particular importance, is to understand how drought will alter the species composition of regenerating temperate forests wherein symbiotic dinitrogen (N-2)-fixing plants play a critical role. In experimental mesocosms we manipulated soil moisture to study the effect of drought on the physiology, growth and competitive interactions of four co-occurring North American tree species, one of which (Robinia pseudoacacia) is a symbiotic N-2-fixer. We hypothesized that drought would reduce growth by decreasing stomatal conductance, hydraulic conductance and increasing the water use efficiency of species with larger diameter xylem vessel elements (Quercus rubra, R. pseudoacacia) relative to those with smaller elements (Acer rubrum and Liriodendron tulipifera). We further hypothesized that N-2 fixation by R. pseudoacacia would decline with drought, reducing its competitive ability. Under drought, growth declined across all species; but, growth and physiological responses did not correspond to species' hydraulic architecture. Drought triggered an 80 % increase in nodule biomass and N accrual for R. pseudoacacia, improving its growth relative to other species. These results suggest that drought intensified soil N deficiency and that R. pseudoacacia's ability to fix N-2 facilitated competition with non-fixing species when both water and N were limiting. Under scenarios of moderate drought, N-2 fixation may alleviate the N constraints resulting from low soil moisture and improve competitive ability of N-2-fixing species, and as a result, supply more new N to the ecosystem. C1 [Wurzburger, Nina] Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. [Miniat, Chelcy Ford] US Forest Serv, Coweeta Hydrol Lab, USDA, Southern Res Stn, Otto, NC 28763 USA. RP Wurzburger, N (reprint author), Univ Georgia, Odum Sch Ecol, Athens, GA 30602 USA. EM ninawurz@uga.edu OI Miniat, Chelcy/0000-0002-3266-9783 FU United States Department of Agriculture Forest Service; Southern Research Station; [11-CA-11330140-095] FX This study was supported by the United States Department of Agriculture Forest Service, Southern Research Station, and by cooperative agreement number 11-CA-11330140-095 to N. Wurzburger at the University of Georgia. Any opinions, findings, conclusions, or recommendations expressed in the material are those of the authors and do not necessarily reflect the views of the USDA Forest Service or the University of Georgia. We thank Neal Muldoon, Shialoh Wilson, Sheena Zhang, Courtney Collins, Steven T. Brantley, and Jeff Minucci for their assistance with this research. We are grateful to Lindsay Boring, Ford Ballantyne and two anonymous reviewers for their constructive comments on the manuscript. NR 54 TC 11 Z9 11 U1 4 U2 51 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2014 VL 174 IS 4 BP 1117 EP 1126 DI 10.1007/s00442-013-2851-0 PG 10 WC Ecology SC Environmental Sciences & Ecology GA AD3UJ UT WOS:000333171400005 PM 24337710 ER PT J AU Peters, DPC Yao, J Browning, D Rango, A AF Peters, Debra P. C. Yao, Jin Browning, Dawn Rango, Albert TI Mechanisms of grass response in grasslands and shrublands during dry or wet periods SO OECOLOGIA LA English DT Article DE Bouteloua eriopoda; Demographic processes; Grasslands; Plant-soil water feedbacks; Sporobolus flexuosus ID RAIN-USE EFFICIENCY; CHIHUAHUAN DESERT; SEMIARID ECOSYSTEMS; CLIMATE VARIABILITY; SHORTGRASS STEPPE; PATTERNS; DESERTIFICATION; VEGETATION; AVAILABILITY; FACILITATION AB Multi-year climatic periods are expected to increase with global change, yet long-term data are often insufficient to document factors leading to ecological responses. We used a suite of long-term datasets (1993-2010) to examine the processes underlying different relationships between aboveground net primary production (ANPP) and precipitation in wet and dry rainfall periods in shrublands and grasslands in the Chihuahuan Desert. We hypothesized that trends in ANPP can be explained by different processes associated with their dominant grasses [Bouteloua eriopoda (grasslands); Sporobolus flexuosus (shrublands)] and with ecosystem properties that influence soil water dynamics with feedbacks to ANPP. We compared datasets on recruitment and growth for 7 years with no trend in precipitation followed by a 4-year drought and 5 consecutive wet years. We integrated these data in a simulation model to examine the importance of positive feedbacks. In grasslands, ANPP was linearly related to precipitation regardless of rainfall period, primarily as a result of stolon recruitment by B. eriopoda. A lag in responses suggests the importance of legacies associated with stolon density. In shrublands, ANPP was only related to rainfall in the wet period when it increased nonlinearly as the number of wet years increased. Seed availability increased in the first wet year, and seedling establishment occurred 2-4 years later. Increases in biomass, litter and simulated transpiration beginning in the third year corresponded with increases in ANPP. Understanding the processes underlying ecosystem dynamics in multi-year dry or wet periods is expected to improve predictions under directional increases or decreases in rainfall. C1 [Peters, Debra P. C.; Yao, Jin; Browning, Dawn; Rango, Albert] New Mexico State Univ, Jornada Basin Long Term Ecol Res Program, Las Cruces, NM 88003 USA. [Peters, Debra P. C.; Browning, Dawn; Rango, Albert] ARS, USDA, Jornada Expt Range, Las Cruces, NM 88003 USA. RP Peters, DPC (reprint author), New Mexico State Univ, Jornada Basin Long Term Ecol Res Program, Las Cruces, NM 88003 USA. EM debpeter@nmsu.edu FU National Science Foundation, Jornada Basin Long Term Ecological Research Program [DEB-1235828]; New Mexico State University [DEB-0917668, EF-1065699]; LTER; USDA-ARS FX We thank Laura Huenneke for designing the long-term studies on plant ANPP and phenology, and John Anderson and the Jornada Basin LTER technicians for collecting the data through time. Kris Havstad provided constructive comments on the manuscript. Funding was provided by the National Science Foundation to New Mexico State University as part of the Jornada Basin Long Term Ecological Research Program (DEB-1235828) and by related awards to New Mexico State University (DEB-0917668, EF-1065699). The Jornada Basin site consists of the Jornada Experimental Range administered by the U.S. Department of Agriculture-Agricultural Research Service, and the Chihuahuan Desert Rangeland Research Center administered by New Mexico State University. The NPP and Phenology studies are funded by the Jornada LTER Program, and the JORNEX Project is jointly funded by the LTER and USDA-ARS. The experiments comply with the current laws of the USA in which the experiments were performed. NR 51 TC 13 Z9 14 U1 3 U2 56 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2014 VL 174 IS 4 BP 1323 EP 1334 DI 10.1007/s00442-013-2837-y PG 12 WC Ecology SC Environmental Sciences & Ecology GA AD3UJ UT WOS:000333171400025 PM 24263235 ER PT J AU Meyer, SE Merrill, KT Allen, PS Beckstead, J Norte, AS AF Meyer, Susan E. Merrill, Katherine T. Allen, Phil S. Beckstead, Julie Norte, Anna S. TI Indirect effects of an invasive annual grass on seed fates of two native perennial grass species SO OECOLOGIA LA English DT Article DE Apparent competition; Bromus tectorum; Facilitation; Seed pathogen; Seedling recruitment ID PYRENOPHORA-SEMENIPERDA; APPARENT COMPETITION; FUNGAL PATHOGENS; PLANT LITTER; ESTABLISHMENT; CHEATGRASS; DYNAMICS; INFECTION; DOMINANCE; COMMUNITY AB Invasive plants exhibit both direct and indirect negative effects on recruitment of natives following invasion. We examined indirect effects of the invader Bromus tectorum (cheatgrass) on seed fates of two native grass species, Elymus elymoides and Pseudoroegneria spicata, by removing B. tectorum and by adding inoculum of the shared seed pathogen Pyrenophora semeniperda in factorial experiments at xeric and mesic field sites. We also included a supplemental watering treatment to increase emergence and also the potential for pathogen escape. We recorded emergence and survival of native seedlings and also determined the fate of unemerged seeds. At the xeric site, Pyrenophora-caused mortality was high (34 %), and effects of other pathogens and failed emergence of germinants were smaller. Cheatgrass removal negatively affected both emergence (35 vs. 25 %) and spring survival (69 vs. 42 %). Pyrenophora-caused seed mortality increased with inoculum augmentation for both species (22 vs. 47 % overall), but emergence was negatively impacted only for P. spicata (20 vs. 34 %). At the mesic site, Pyrenophora-caused mortality was low (6 %). Cheatgrass removal doubled emergence (26 vs. 14 %). Seed mortality increased significantly with inoculum augmentation for P. spicata (12 vs. 5 %) but not E. elymoides, while emergence was not significantly affected in either species. A large fraction of seeds produced germinants that failed to emerge (37 %), while another large fraction (35 %) was killed by other pathogens. We conclude that facilitation by cheatgrass at the xeric site but interference at the mesic site was probably mediated through litter effects that could be ameliorative or suppressive. Apparent competition between cheatgrass and native grasses could occur through Pyrenophora, especially in a xeric environment, but effects were weak or absent at emergence. This was probably because Pyrenophora attacks the same slow-germinating fraction that is subject to pre-emergence mortality from other causes, including attack by other pathogens such as Fusarium. C1 [Meyer, Susan E.] US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, USDA, Provo, UT 84606 USA. [Merrill, Katherine T.; Allen, Phil S.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. [Beckstead, Julie; Norte, Anna S.] Gonzaga Univ, Dept Biol, Spokane, WA 99258 USA. RP Meyer, SE (reprint author), US Forest Serv, Shrub Sci Lab, Rocky Mt Res Stn, USDA, 735 North,500 East, Provo, UT 84606 USA. EM smeyer@fs.fed.us FU USDA-CSRES National Research Initiative [2008-35320-18677]; Joint Fire Science Program [2007-1-3-10]; Bureau of Land Management Integrated Cheat-grass Die-off Grant FX This work was funded in part by USDA-CSRES National Research Initiative Grant no. 2008-35320-18677 and Joint Fire Science Program Grant no. 2007-1-3-10, both to S. E. M., P. S. A. and J. B. The Packer Creek follow-up study was funded through a Bureau of Land Management Integrated Cheat-grass Die-off Grant to S. E. M. and J. B. These experiments comply with the legal requirements of the country in which they were performed. The authors declare no conflict of interest with the funding agencies. Thanks to the BLM Spokane District for access to the Packer Creek site and the BLM Salt Lake District for access to the Whiterocks site. We thank Glenn Paulsen for providing water and fencing at Packer Creek, Suzette Clement for preparation of bulk inoculum, Stephanie Carlson, Suzette Clement, Heather Finch, Keith Merrill, Bettina Schultz, Abbey Shuster, Katherine Horlick, Kelsey McEvoy, and Kaitlin Van Volkom for assistance with field experiments, and Desiree Lara and JanaLynn Franke for molecular-genetic identifications. NR 43 TC 6 Z9 6 U1 3 U2 37 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0029-8549 EI 1432-1939 J9 OECOLOGIA JI Oecologia PD APR PY 2014 VL 174 IS 4 BP 1401 EP 1413 DI 10.1007/s00442-013-2868-4 PG 13 WC Ecology SC Environmental Sciences & Ecology GA AD3UJ UT WOS:000333171400032 PM 24399482 ER PT J AU Rolshausen, PE Baumgartner, K Travadon, R Fujiyoshi, P Pouzoulet, J Wilcox, WF AF Rolshausen, Philippe E. Baumgartner, Kendra Travadon, Renaud Fujiyoshi, Phillip Pouzoulet, Jerome Wilcox, Wayne F. TI Identification of Eutypa spp. Causing Eutypa Dieback of Grapevine in Eastern North America SO PLANT DISEASE LA English DT Article ID GENETIC-STRUCTURE; WOOD CANKERS; LATA; PATHOGEN; CALIFORNIA; ARMENIACAE; VINEYARDS; DIVERSITY; VITIS; ASCOSPORES AB Eutypa dieback of grapevine is caused by Eutypa lata in production areas with Mediterranean climates in California, Australasia, Europe, and South Africa. Eutypa dieback has also been described in the colder, eastern North American vineyards where cultivars adapted from native Vitis spp. (e.g., Vitis x labruscana 'Concord') are primarily grown. However, the causal agents associated with the diseases in this region have not been conclusively identified. Examination of 48 vineyards showing symptoms of dieback in the northeastern United States (Connecticut, Massachusetts, Michigan, New York, Ohio, and Rhode Island) and Ontario, Canada revealed that vineyards were mainly infected by Eutypa spp. other than E. lata. Multigene phylogenies (internal transcribed spacer ribosomal DNA, P-tubulin, and RNA polymerase II) of isolates recovered from these vineyards indicated that Eutypa dieback is caused primarily by an undescribed Eutypa sp. and E. laevata. Eutypa sp. was recovered from 56% of the vineyards examined, whereas E. laevata and E. lata were less far common (17 and 6%, respectively). Fruiting body morphology and spore dimensions supported phylogenetic separation of the three taxa. Pathogenicity tests conducted on Vitis vitufera 'Chardonnay' in the greenhouse and in the field verified that all three species were able to cause wood canker and to infect pruning wounds, respectively. C1 [Rolshausen, Philippe E.; Pouzoulet, Jerome] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Baumgartner, Kendra; Fujiyoshi, Phillip] USDA ARS, Davis, CA 95616 USA. [Travadon, Renaud] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Wilcox, Wayne F.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY 14456 USA. RP Rolshausen, PE (reprint author), Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. EM philrols@ucr.edu FU USDA, National Institute of Food and Agriculture [2008-51100-19334] FX Research was funded by grant number 2008-51100-19334 to K. Baumgartner and P. E. Rolshausen from the USDA, National Institute of Food and Agriculture. We thank B. Mackey (Statistician, USDA-ARS, Albany, CA) for advice on statistical analyses; A. Schilder (Michigan State University), M. Ellis (Ohio State University), and W. McFadden-Smith (Ontario Ministry of Agriculture) for their assistance; vineyard owners for access to field sites; and the Foundation Plant Services in Davis, CA, for the donation of grapevine cuttings. NR 48 TC 2 Z9 2 U1 1 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD APR PY 2014 VL 98 IS 4 BP 483 EP 491 DI 10.1094/PDIS-08-13-0883-RE PG 9 WC Plant Sciences SC Plant Sciences GA AD7IG UT WOS:000333436700006 ER PT J AU Wu, YZ Yan, SJ Lv, Z Chen, L Geng, JS He, JJ Yu, QZ Yin, JC Ren, GP Li, DS AF Wu, Yunzhou Yan, Shijun Lv, Zheng Chen, Lin Geng, Jingshu He, Jinjiao Yu, Qingzhong Yin, Jiechao Ren, Guiping Li, Deshan TI Recombinant Newcastle Disease virus Anhinga Strain (NDV/Anh-EGFP) for Hepatoma Therapy SO TECHNOLOGY IN CANCER RESEARCH & TREATMENT LA English DT Article DE Newcastle disease virus; Oncolysis; Hepatoma; Hepatocellular carcinoma ID MALIGNANT-MELANOMA; HEPATOCELLULAR-CARCINOMA; VIRAL ONCOLYSATE; CANCER-THERAPY; FOLLOW-UP; VACCINE; INFECTION; PROTEIN; DETERMINANT; MANAGEMENT AB Hepatocellular carcinoma remains one of the most common malignant tumors in the world. Newcastle disease virus (NDV) has been proved to be an efficient oncolytic agent. NDV tumor killing efficacy is not only dependening on the NDV strain but the type of tumor targeted. It is significant to discover more effective and safe oncolytic strains. We investigated the effectiveness of genetically engineered NDV Anhinga strain in hepatoma treatment. The modified virus containing an insertion of enhanced green fluorescent protein (EGFP), named NDV/ Anh-EGFP. The antitumor efficacy of the recombinant virus on hepatoma was examined both in vivo and in vitro. NDV Anhinga strain, which could be classified as a lytic strain, is an effective oncolytic agent on hepatoma. There was no significant difference in the TCID50 and growth capability between the recombinant NDV and the parental. NDV/Anh-EGFP can obviously inhibit hepatocarcinoma development in vitro and in vivo. We demonstrate Anhinga strain could become a potent candidate for clinical carcinoma therapy especially for hepatocarcinoma. C1 [Wu, Yunzhou; Yan, Shijun; Lv, Zheng; He, Jinjiao; Yin, Jiechao; Ren, Guiping; Li, Deshan] Northeast Agr Univ, Coll Life Sci, Harbin, Peoples R China. [Chen, Lin] Harbin Med Univ, Affiliated Tumor Hosp, Ctr Radiat Treatment, Harbin, Peoples R China. [Geng, Jingshu] Harbin Med Univ, Affiliated Tumor Hosp, Dept Pathol, Harbin, Peoples R China. [Yu, Qingzhong] ARS, Southeast Poultry Res Lab, USDA, Athens, GA 30605 USA. RP Li, DS (reprint author), Northeast Agr Univ, Coll Life Sci, Mucai St 59, Harbin, Peoples R China. EM deshanli@163.com NR 30 TC 4 Z9 4 U1 0 U2 5 PU ADENINE PRESS PI SCHENECTADY PA 2066 CENTRAL AVE, SCHENECTADY, NY 12304 USA SN 1533-0346 EI 1533-0338 J9 TECHNOL CANCER RES T JI Technol. Cancer Res. Treat. PD APR PY 2014 VL 13 IS 2 BP 169 EP 175 DI 10.7785/tcrt.2012.500356 PG 7 WC Oncology SC Oncology GA AD4NC UT WOS:000333226800009 PM 23819497 ER PT J AU Zambrano, JL Jones, MW Brenner, E Francis, DM Tomas, A Redinbaugh, MG AF Luis Zambrano, Jose Jones, Mark W. Brenner, Eric Francis, David M. Tomas, Adriana Redinbaugh, Margaret G. TI Genetic analysis of resistance to six virus diseases in a multiple virus-resistant maize inbred line SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID DWARF-MOSAIC-VIRUS; ZEA-MAYS L.; RIO-CUARTO DISEASE; RAYADO-FINO-VIRUS; STREAK-VIRUS; LEAFHOPPER TRANSMISSION; POTYVIRUS RESISTANCE; LETHAL NECROSIS; SCMV RESISTANCE; CARRYING WSM1 AB Novel and previously known resistance loci for six phylogenetically diverse viruses were tightly clustered on chromosomes 2, 3, 6 and 10 in the multiply virus-resistant maize inbred line, Oh1VI. Virus diseases in maize can cause severe yield reductions that threaten crop production and food supplies in some regions of the world. Genetic resistance to different viruses has been characterized in maize populations in diverse environments using different screening techniques, and resistance loci have been mapped to all maize chromosomes. The maize inbred line, Oh1VI, is resistant to at least ten viruses, including viruses in five different families. To determine the genes and inheritance mechanisms responsible for the multiple virus resistance in this line, F-1 hybrids, F-2 progeny and a recombinant inbred line (RIL) population derived from a cross of Oh1VI and the virus-susceptible inbred line Oh28 were evaluated. Progeny were screened for their responses to Maize dwarf mosaic virus, Sugarcane mosaic virus, Wheat streak mosaic virus, Maize chlorotic dwarf virus, Maize fine streak virus, and Maize mosaic virus. Depending on the virus, dominant, recessive, or additive gene effects were responsible for the resistance observed in F-1 plants. One to three gene models explained the observed segregation of resistance in the F-2 generation for all six viruses. Composite interval mapping in the RIL population identified 17 resistance QTLs associated with the six viruses. Of these, 15 were clustered in specific regions of chr. 2, 3, 6, and 10. It is unknown whether these QTL clusters contain single or multiple virus resistance genes, but the coupling phase linkage of genes conferring resistance to multiple virus diseases in this population could facilitate breeding efforts to develop multi-virus resistant crops. C1 [Luis Zambrano, Jose; Francis, David M.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Hort & Crop Sci, Wooster, OH 44691 USA. [Luis Zambrano, Jose] Inst Nacl Autonomo Invest Agr INIAP, Programa Nacl Maiz, Quito, Ecuador. [Jones, Mark W.; Brenner, Eric; Redinbaugh, Margaret G.] Ohio State Univ, Ohio Agr Res & Dev Ctr, ARS, USDA,Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. [Jones, Mark W.; Brenner, Eric; Redinbaugh, Margaret G.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Dept Plant Pathol, Wooster, OH 44691 USA. [Tomas, Adriana] DuPont Agr Biotechnol, Genet Discovery, Wilmington, DE 19880 USA. RP Redinbaugh, MG (reprint author), Ohio State Univ, Ohio Agr Res & Dev Ctr, ARS, USDA,Corn Soybean & Wheat Qual Res Unit, Wooster, OH 44691 USA. EM peg.redinbaugh@ars.usda.gov RI Redinbaugh, Margaret/A-3611-2013; Francis, David/B-7262-2012 FU Instituto Nacional Autonomo de Investigaciones Agropecuarias (INIAP), Ecuador; State and Federal funds; Ohio Agricultural Research and development Center, The Ohio State University FX We thank William Belote (Dupont, Stine-Haskell Research Center) for providing a P. maidis colony and to J. Todd (USDA-ARS) for maintaining the insect colonies. We also thank Geoff Parker (Ohio State University) for technical assistance with the SSR genotyping and Brayton Orchard (Ohio State University) for providing the Circos scripts for the QTL graph. JLZ thanks the Instituto Nacional Autonomo de Investigaciones Agropecuarias (INIAP), Ecuador for a fellowship to support his Ph.D. study. Salaries and research support were provided in part by State and Federal funds appropriated to the Ohio Agricultural Research and development Center, The Ohio State University. NR 70 TC 12 Z9 13 U1 3 U2 35 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2014 VL 127 IS 4 BP 867 EP 880 DI 10.1007/s00122-014-2263-5 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AD6FZ UT WOS:000333353400009 PM 24500307 ER PT J AU Zhou, XL Wang, MN Chen, XM Lu, Y Kang, ZS Jing, JX AF Zhou, X. L. Wang, M. N. Chen, X. M. Lu, Y. Kang, Z. S. Jing, J. X. TI Identification of Yr59 conferring high-temperature adult-plant resistance to stripe rust in wheat germplasm PI 178759 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; ANALOG POLYMORPHISM MARKERS; PUCCINIA-STRIIFORMIS; SEEDLING RESISTANCE; NORTH-AMERICA; UNITED-STATES; GENE; CULTIVARS; RACES; BARLEY AB This manuscript reports a new gene for non-race-specific resistance to stripe rust and molecular markers for incorporating it into wheat cultivars for control of the disease with durable resistance. Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive wheat diseases worldwide. The spring wheat germplasm 'PI 178759' originating from Iraq showed effective resistance to stripe rust in field evaluations over 8 years in Washington state, USA. To map the resistance gene(s), PI 178759 was crossed with 'Avocet Susceptible', and the parents and 176 F-2:3 lines were phenotyped in the fields under natural infection and in a greenhouse with selected races of P. striiformis f. sp. tritici. PI 178759 was identified to have high-temperature adult-plant (HTAP) resistance. Resistance gene analog polymorphism and simple sequence repeat techniques were used to identify molecular markers linked to the resistance gene and a chromosome region was mapped using a quantitative trait locus approach. One major gene was mapped to the long arm of chromosome 7B. Flanked by Xwgp5175 and Xbarc32 in a 2.1 cM region, the gene explained 31.8 and 54.7 % of the phenotypic variation in rAUDPC and IT, respectively. Based on genetic distances among markers and allelism tests, the HTAP resistance gene in PI 178759 is different from the previously reported Yr39, Yr52, YrZH84, and YrC591, also located on chromosome 7BL, and is therefore designated as Yr59. The gene and its flanking markers should be useful for developing wheat cultivars with durable resistance. C1 [Zhou, X. L.; Lu, Y.; Kang, Z. S.; Jing, J. X.] Northwest A&F Univ, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China. [Zhou, X. L.; Lu, Y.; Kang, Z. S.; Jing, J. X.] Northwest A&F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China. [Zhou, X. L.; Wang, M. N.; Chen, X. M.; Lu, Y.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Chen, X. M.] ARS, Wheat Genet Qual Physiol & Dis Res Unit, USDA, Pullman, WA 99164 USA. RP Chen, XM (reprint author), ARS, Wheat Genet Qual Physiol & Dis Res Unit, USDA, Pullman, WA 99164 USA. EM xianming@wsu.edu RI WANG, Meinan/P-5671-2014 OI WANG, Meinan/0000-0001-9595-3995 FU US Department of Agriculture, Agricultural Research Service [5348-22000-015-00D]; National Key Basic Research Program of China [2013CB127700]; Washington Wheat Commission [13C-3061-3923]; Vogel Foundation [13Z-3061-3824]; China Scholarship Council; Northwest A&F University Plant Pathology "111'' Project [B07049] FX This research was supported by the US Department of Agriculture, Agricultural Research Service (Project No. 5348-22000-015-00D), National Key Basic Research Program of China (2013CB127700), Washington Wheat Commission (Project No. 13C-3061-3923) and Vogel Foundation (Project No. 13Z-3061-3824). We thank Ms. Lu Hou for technical assistance and Dr. Robert McIntosh for critical review of the manuscript. A scholarship to Xinli Zhou from the China Scholarship Council was greatly appreciated. The research is also part of a Northwest A&F University Plant Pathology "111'' Project (B07049). NR 37 TC 21 Z9 26 U1 8 U2 50 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2014 VL 127 IS 4 BP 935 EP 945 DI 10.1007/s00122-014-2269-z PG 11 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AD6FZ UT WOS:000333353400014 PM 24487945 ER PT J AU Niu, Z Klindworth, DL Yu, G Friesen, TL Chao, S Jin, Y Cai, X Ohm, JB Rasmussen, JB Xu, SS AF Niu, Z. Klindworth, D. L. Yu, G. Friesen, T. L. Chao, S. Jin, Y. Cai, X. Ohm, J-B. Rasmussen, J. B. Xu, Steven S. TI Development and characterization of wheat lines carrying stem rust resistance gene Sr43 derived from Thinopyrum ponticum SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID F-SP TRITICI; TRANSLOCATIONS CONFERRING RESISTANCE; AGROPYRON-ELONGATUM; RACE TTKS; CYTOGENETIC CHARACTERIZATION; LR19 TRANSLOCATION; PHYSICAL MAP; BREAD WHEAT; DURUM-WHEAT; UG99 RACES AB Wheat lines carrying Ug99-effective stem rust resistance gene Sr43 on shortened alien chromosome segments were produced using chromosome engineering, and molecular markers linked to Sr43 were identified for marker-assisted selection. Stem rust resistance gene Sr43, transferred into common wheat (Triticum aestivum) from Thinopyrum ponticum, is an effective gene against stem rust Ug99 races. However, this gene has not been used in wheat breeding because it is located on a large Th. ponticum 7el(2) chromosome segment, which also harbors genes for undesirable traits. The objective of this study was to eliminate excessive Th. ponticum chromatin surrounding Sr43 to make it usable in wheat breeding. The two original translocation lines KS10-2 and KS24-1 carrying Sr43 were first analyzed using simple sequence repeat (SSR) markers and florescent genomic in situ hybridization. Six SSR markers located on wheat chromosome arm 7DL were identified to be associated with the Th. ponticum chromatin in KS10-2 and KS24-1. The results confirmed that KS24-1 is a 7DS center dot 7el(2)L Robertsonian translocation as previously reported. However, KS10-2, which was previously designated as a 7el(2)S center dot 7el(2)L-7DL translocation, was identified as a 7DS-7el(2)S center dot 7el(2)L translocation. To reduce the Th. ponticum chromatin carrying Sr43, a BC2F1 population (Chinese Spring//Chinese Spring ph1bph1b*2/KS10-2) containing ph1b-induced homoeologous recombinants was developed, tested with stem rust, and genotyped with the six SSR markers identified above. Two new wheat lines (RWG33 and RWG34) carrying Sr43 on shortened alien chromosome segments (about 17.5 and 13.7 % of the translocation chromosomes, respectively) were obtained, and two molecular markers linked to Sr43 in these lines were identified. The new wheat lines with Sr43 and the closely linked markers provide new resources for improving resistance to Ug99 and other races of stem rust in wheat. C1 [Niu, Z.; Klindworth, D. L.; Friesen, T. L.; Chao, S.; Ohm, J-B.; Xu, Steven S.] USDA ARS, No Crop Sci Lab, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Yu, G.; Rasmussen, J. B.] N Dakota State Univ, Dept Plant Pathol, Fargo, ND 58108 USA. [Jin, Y.] USDA ARS, Cereal Dis Lab, St Paul, MN 55108 USA. [Cai, X.] N Dakota State Univ, Dept Plant Sci, Fargo, ND 58108 USA. RP Xu, SS (reprint author), USDA ARS, No Crop Sci Lab, Cereal Crops Res Unit, 1605 Albrecht Blvd North, Fargo, ND 58102 USA. EM steven.xu@ars.usda.gov FU Bill & Melinda Gates Foundation; USDA-ARS CRIS Project [5442-22000-037-00D] FX We thank Dr. G. Francois Marais and Dr. Lili Qi for critically reviewing the manuscript. The authors also thank Mary Osenga, Rachel McArthur, Danielle Holmes, and Xiaohong Jiang for technical support. This research was supported in part by funds to S. S. X. provided through a grant from the Bill & Melinda Gates Foundation to Cornell University for the Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) Project and the USDA-ARS CRIS Project No. 5442-22000-037-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 56 TC 23 Z9 25 U1 3 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD APR PY 2014 VL 127 IS 4 BP 969 EP 980 DI 10.1007/s00122-014-2272-4 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AD6FZ UT WOS:000333353400017 PM 24504553 ER PT J AU Samaniuk, JR Shay, TW Root, TW Klingenberg, DJ Scott, CT AF Samaniuk, Joseph R. Shay, Timothy W. Root, Thatcher W. Klingenberg, Daniel J. Scott, C. Tim TI A novel rheometer design for yield stress fluids SO AICHE JOURNAL LA English DT Article DE lignocellulosic biomass; rheometer; rheology; auger; yield stress ID ENZYMATIC-HYDROLYSIS; NONCOLLOIDAL PARTICLES; RHEOLOGICAL PROPERTIES; FIBER SUSPENSIONS; BIOMASS SLURRIES; ETHANOL; FLOW AB An inexpensive, rapid method for measuring the rheological properties of yield stress fluids is described and tested. The method uses an auger that does not rotate during measurements, and avoids material and instrument-related difficulties, for example, wall slip and the presence of large particles, associated with yield stress fluids. The method can be used for many types of yield stress fluids, including concentrated lignocellulosic biomass. Sample preparation prior to measurement is minimal, reducing, or eliminating disruption of the sample. We show that measurements using this technique compare well with measurements obtained with a vane rheometer. A variation of the described method is proposed that would make it easier to measure time-dependent rheological properties. (c) 2014 American Institute of Chemical Engineers AIChE J, 60: 1523-1528, 2014 C1 [Samaniuk, Joseph R.; Shay, Timothy W.; Root, Thatcher W.; Klingenberg, Daniel J.] Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI 53706 USA. [Scott, C. Tim] US Forest Serv, USDA, Madison, WI 53726 USA. RP Klingenberg, DJ (reprint author), Univ Wisconsin, Dept Chem & Biol Engn, Madison, WI 53706 USA. EM klingen@engr.wisc.edu RI Samaniuk, Joseph/C-4773-2014 FU U.S. Department of Agriculture (NRI award) [2006-35504-17401]; U.S. Department of Agriculture (AFRI award) [2010-65504-20406] FX This project was supported by the U.S. Department of Agriculture (NRI award number 2006-35504-17401 and AFRI award number 2010-65504-20406). NR 32 TC 0 Z9 0 U1 3 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0001-1541 EI 1547-5905 J9 AICHE J JI AICHE J. PD APR PY 2014 VL 60 IS 4 BP 1523 EP 1528 DI 10.1002/aic.14329 PG 6 WC Engineering, Chemical SC Engineering GA AC5YH UT WOS:000332596400030 ER PT J AU Hori, C Gaskell, J Igarashi, K Kersten, P Mozuch, M Samejima, M Cullen, D AF Hori, Chiaki Gaskell, Jill Igarashi, Kiyohiko Kersten, Phil Mozuch, Michael Samejima, Masahiro Cullen, Dan TI Temporal Alterations in the Secretome of the Selective Ligninolytic Fungus Ceriporiopsis subvermispora during Growth on Aspen Wood Reveal This Organism's Strategy for Degrading Lignocellulose SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID GLYCOSIDE HYDROLASE FAMILY; ARYL-ALCOHOL OXIDASE; PHANEROCHAETE-CHRYSOSPORIUM; MASS-SPECTROMETRY; CELLULOSE DEPOLYMERIZATION; CELLOBIOSE DEHYDROGENASE; SUBSTRATE-SPECIFICITY; MANGANESE PEROXIDASE; CATALYTIC-PROPERTIES; ENZYMES AB The white-rot basidiomycetes efficiently degrade all wood cell wall polymers. Generally, these fungi simultaneously degrade cellulose and lignin, but certain organisms, such as Ceriporiopsis subvermispora, selectively remove lignin in advance of cellulose degradation. However, relatively little is known about the mechanism of selective ligninolysis. To address this issue, C. subvermispora was grown in liquid medium containing ball-milled aspen, and nano-liquid chromatography-tandem mass spectrometry was used to identify and estimate extracellular protein abundance over time. Several manganese peroxidases and an aryl alcohol oxidase, both associated with lignin degradation, were identified after 3 days of incubation. A glycoside hydrolase (GH) family 51 arabinofuranosidase was also identified after 3 days but then successively decreased in later samples. Several enzymes related to cellulose and xylan degradation, such as GH10 endoxylanase, GH5_5 endoglucanase, and GH7 cellobiohydrolase, were detected after 5 days. Peptides corresponding to potential cellulose-degrading enzymes GH12, GH45, lytic polysaccharide monooxygenase, and cellobiose dehydrogenase were most abundant after 7 days. This sequential production of enzymes provides a mechanism consistent with selective ligninolysis by C. subvermispora. C1 [Hori, Chiaki; Gaskell, Jill; Kersten, Phil; Mozuch, Michael; Cullen, Dan] USDA, Forest Prod Lab, Madison, WI 53705 USA. [Hori, Chiaki; Igarashi, Kiyohiko; Samejima, Masahiro] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomat Sci, Tokyo, Japan. RP Cullen, D (reprint author), USDA, Forest Prod Lab, Madison, WI 53705 USA. EM dcullen@wisc.edu RI Igarashi, Kiyohiko/E-6799-2016 OI Igarashi, Kiyohiko/0000-0001-5152-7177 FU Japan Society for the Promotion of Science (JSPS) [251143]; National Research Initiative of the USDA Cooperative State Research, Education and Extension Service [2007-35504-18257]; Agriculture and Food Research Research Initiative from the USDA National Institute of Food and Agriculture [2011-67009-20056] FX This research was supported by a research fellowship to C. H. (no. 251143) from the Japan Society for the Promotion of Science (JSPS), by the National Research Initiative of the USDA Cooperative State Research, Education and Extension Service (grant 2007-35504-18257) to D. C., and by the Agriculture and Food Research Research Initiative (grant 2011-67009-20056) from the USDA National Institute of Food and Agriculture to P. K. NR 48 TC 16 Z9 17 U1 1 U2 56 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2014 VL 80 IS 7 BP 2062 EP 2070 DI 10.1128/AEM.03652-13 PG 9 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AC9FV UT WOS:000332840700003 PM 24441164 ER PT J AU Welsh, A Chee-Sanford, JC Connor, LM Loffler, FE Sanford, RA AF Welsh, Allana Chee-Sanford, Joanne C. Connor, Lynn M. Loeffler, Frank E. Sanford, Robert A. TI Refined NrfA Phylogeny Improves PCR-Based nrfA Gene Detection SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID C NITRITE REDUCTASE; DISSIMILATORY NITRATE REDUCTION; MULTIPLE SEQUENCE ALIGNMENT; NOV SP-NOV; NITROGEN-CYCLE; WOLINELLA-SUCCINOGENES; CAMPYLOBACTER-JEJUNI; ESCHERICHIA-COLI; CYTOCHROME; EVOLUTION AB Dissimilatory nitrate reduction to ammonium (DNRA) and denitrification are contrasting microbial processes in the terrestrial nitrogen (N) cycle, in that the former promotes N retention and the latter leads to N loss (i.e., the formation of gaseous products). The nitrite reductase NrfA catalyzes nitrite reduction to ammonium, the enzyme associated with respiratory nitrite ammonification and the key step in DNRA. Although well studied biochemically, the diversity and phylogeny of this enzyme had not been rigorously analyzed. A phylogenetic analysis of 272 full-length NrfA protein sequences distinguished 18 NrfA clades with robust statistical support (>90% Bayesian posterior probabilities). Three clades possessed a CXXCH motif in the first heme-binding domain, whereas all other clades had a CXXCK motif in this location. The analysis further identified a KXRH or KXQH motif between the third and fourth heme-binding motifs as a conserved and diagnostic feature of all pentaheme NrfA proteins. PCR primers targeting a portion of the heme-binding motifs that flank this diagnostic region yielded the expected 250-bp-long amplicons with template DNA from eight pure cultures and 16 new nrfA-containing isolates. nrfA amplicons obtained with template DNA from two geomorphically distinct agricultural soils could be assigned to one of the 18 NrfA clades, providing support for this expanded classification. The extended NrfA phylogeny revealed novel diagnostic features of DNRA populations and will be useful to assess nitrate/nitrite fate in natural and engineered ecosystems. C1 [Welsh, Allana; Chee-Sanford, Joanne C.; Sanford, Robert A.] Univ Illinois, Urbana, IL 61801 USA. [Chee-Sanford, Joanne C.; Connor, Lynn M.] USDA ARS, Urbana, IL USA. [Loeffler, Frank E.] Univ Tennessee, Dept Microbiol, Knoxville, TN 37996 USA. [Loeffler, Frank E.] Univ Tennessee, Ctr Environm Biotechnol, Knoxville, TN 37932 USA. [Loeffler, Frank E.] Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN USA. [Loeffler, Frank E.] Univ Tennessee, Oak Ridge, TN USA. [Loeffler, Frank E.] JIBS, Oak Ridge Natl Lab UT ORNL, Oak Ridge, TN USA. [Loeffler, Frank E.] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA. RP Welsh, A (reprint author), Univ Illinois, Urbana, IL 61801 USA. EM welsh@illinois.edu FU U.S. Department of Energy, Office of Biological and Environmental Research, Genomic Science Program [DE-SC0006662]; USDA-ARS CRIS [3611-12220-008-00D] FX This research was supported by the U.S. Department of Energy, Office of Biological and Environmental Research, Genomic Science Program, award DE-SC0006662, and USDA-ARS CRIS Project 3611-12220-008-00D. NR 75 TC 17 Z9 17 U1 5 U2 58 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2014 VL 80 IS 7 BP 2110 EP 2119 DI 10.1128/AEM.03443-13 PG 10 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AC9FV UT WOS:000332840700008 PM 24463965 ER PT J AU Wang, DP Xu, SX Yang, D Young, GM Tian, P AF Wang, Dapeng Xu, Shuxia Yang, David Young, Glenn M. Tian, Peng TI New In Situ Capture Quantitative (Real-Time) Reverse Transcription-PCR Method as an Alternative Approach for Determining Inactivation of Tulane Virus SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID RECEPTOR-BINDING CAPTURE; PORCINE GASTRIC MUCIN; HUMAN NOROVIRUS; MAGNETIC BEADS; IMMUNO-PCR; ASSAY; PARTICLES; SURROGATE; SYSTEM AB Human noroviruses (HuNoVs) are the major cause of epidemic nonbacterial gastroenteritis. Although quantitative (real-time) reverse transcription-PCR (qRT-PCR) is widely used for detecting HuNoVs, it only detects the presence of viral RNA and does not indicate viral infectivity. Human blood group antigens (HBGAs) have been identified as receptors/co-receptors for both HuNoVs and Tulane virus (TV) and are crucial for viral infection. We propose that viral infectivity can be evaluated with a molecular assay based on receptor-captured viruses. In this study, we employed TV as an HuNoV surrogate to validate the HBGA-based capture qRT-PCR method against the 50% tissue culture infectious dose (TCID50) method. We employed type B HBGA on an immuno-well module to concentrate TV, followed by amplification of the captured viral genome by in situ qRT-PCR. We first demonstrated that this in situ capture qRT-PCR (ISC-qRT-PCR) method could effectively concentrate and detect TV. We then treated TV under either partial or full inactivation conditions and measured the remaining infectivity by ISC-qRT-PCR and a tissue culture-based amplification method (TCID50). We found that the ISC-qRT-PCR method could be used to evaluate virus inactivation deriving from damage to the capsid and study interactions between the capsid and viral receptor. Heat, chlorine, and ethanol treatment primarily affect the capsid structure, which in turns affects the ability of the capsid to bind to viral receptors. Inactivation of the virus by these methods could be reflected by the ISC-qRT-PCR method and confirmed by TCID50 assay. However, the loss of the infectivity caused by damage to the viral genome (such as that from UV irradiation) could not be effectively reflected by this method. Despite this limitation, the ISC-qRT-PCR provides an alternative approach to determine inactivation of Tulane virus. A particular advantage of the ISC-qRT-PCR method is that it is also a faster and easier method to effectively recover and detect the viruses, as there is no need to extract viral RNA or to transfer the captured virus from magnetic beads to PCR tubes for further amplification. Therefore, ISC-qRT-PCR can be easily adapted for use in automated systems for multiple samples. C1 [Wang, Dapeng] Shanghai Jiao Tong Univ, MOST USDA Joint Res Ctr Food Safety, Shanghai 200030, Peoples R China. [Wang, Dapeng] Shanghai Jiao Tong Univ, Sch Agr & Biol, Bor Luh Food Safety Ctr, Shanghai 200030, Peoples R China. [Wang, Dapeng] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Shanghai 200030, Peoples R China. [Wang, Dapeng; Xu, Shuxia; Yang, David; Tian, Peng] ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Xu, Shuxia] Henan Agr Univ, Coll Life Sci, Dept Microbiol, Zhengzhou, Peoples R China. [Young, Glenn M.] Univ Calif Davis, Food Sci & Technol Dept, Davis, CA 95616 USA. RP Tian, P (reprint author), ARS, Produce Safety & Microbiol Res Unit, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM peng.tian@ars.usda.gov FU Ministry of Science and Technology of China [2012AA101601]; National Natural Science Foundation of China [31000063]; USDA Agricultural Research Service CRIS [5325-42000-046-06R] FX This work was jointly supported by the Ministry of Science and Technology of China (grant no. 2012AA101601), the National Natural Science Foundation of China (grant no. 31000063), and USDA Agricultural Research Service CRIS project 5325-42000-046-06R. NR 21 TC 7 Z9 8 U1 1 U2 29 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2014 VL 80 IS 7 BP 2120 EP 2124 DI 10.1128/AEM.04036-13 PG 5 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AC9FV UT WOS:000332840700009 PM 24463967 ER PT J AU Ye, M Li, XH Kingsley, DH Jiang, X Chen, HQ AF Ye, Mu Li, Xinhui Kingsley, David H. Jiang, Xi Chen, Haiqiang TI Inactivation of Human Norovirus in Contaminated Oysters and Clams by High Hydrostatic Pressure SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID HEPATITIS-A VIRUS; REVERSE TRANSCRIPTION-PCR; PORCINE GASTRIC MUCIN; MURINE NOROVIRUS-1; FELINE CALICIVIRUS; VIBRIO-VULNIFICUS; CRASSOSTREA-GIGAS; SHELLFISH; PERSISTENCE; GASTROENTERITIS AB Human norovirus (NoV) is the most frequent causative agent of food-borne disease associated with shellfish consumption. In this study, the effect of high hydrostatic pressure (HHP) on inactivation of NoV was determined. Genogroup I.1 (GI.1) or genogroup II.4 (GII.4) NoV was inoculated into oyster homogenates and treated at 300 to 600 MPa at 25, 6, and 1 degrees C for 5 min. After HHP, samples were treated with RNase and viral particles were extracted with porcine gastric mucin (PGM)-conjugated magnetic beads (PGM-MBs). Viral RNA was then quantified by real-time reverse transcription (RT)-PCR. Since PGM contains histoblood group-like antigens, which can act as receptors for NoV, deficiency for binding to PGM is an indication of loss of infectivity of NoV. After binding to PGM-MBs, RT-PCR-detectable NoV RNA in oysters was reduced by 0.4 to >4 log(10) by HHP at 300 to 600 MPa. The GI.1 NoV was more resistant to HHP than the GII.4 NoV (P < 0.05). HHP at lower temperatures significantly enhanced the inactivation of NoV in oysters (P < 0.05). Pressure treatment was also conducted for clam homogenates. Treatment at 450 MPa at 1 degrees C achieved a >4 log(10) reduction of GI.1 NoV in both oyster and clam homogenates. It is therefore concluded that HHP could be applied as a potential intervention for inactivating NoV in raw shellfish. The method of pretreatment of samples with RNase, extraction of viral particles using PGM-MB binding, and quantification of viral RNA using RT-PCR can be explored as a practical means of distinguishing between infectious and noninfectious NoV. C1 [Ye, Mu; Li, Xinhui; Chen, Haiqiang] Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. [Kingsley, David H.] Delaware State Univ, Food Safety & Intervent Technol Res Unit, James WW Baker Ctr, USDA,ARS, Dover, DE USA. [Jiang, Xi] Univ Cincinnati, Coll Med, Cincinnati Childrens Hosp Med Ctr, Div Infect Dis, Cincinnati, OH USA. RP Chen, HQ (reprint author), Univ Delaware, Dept Anim & Food Sci, Newark, DE 19716 USA. EM haiqiang@udel.edu OI Ye, Mu/0000-0003-0178-5308 FU Agriculture and Food Research Initiative Competitive Grants Program of the USDA National Institute of Food and Agriculture, NIFA [2011-68003-30005] FX This project was supported by the Agriculture and Food Research Initiative Competitive Grants Program of the USDA National Institute of Food and Agriculture, NIFA Award no. 2011-68003-30005. NR 38 TC 14 Z9 14 U1 0 U2 34 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD APR PY 2014 VL 80 IS 7 BP 2248 EP 2253 DI 10.1128/AEM.04260-13 PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AC9FV UT WOS:000332840700023 PM 24487534 ER PT J AU Tillman, GE Simmons, M Garrish, JK Seal, BS AF Tillman, Glenn E. Simmons, Mustafa Garrish, Johnna K. Seal, Bruce S. TI Expression of a Clostridium perfringens genome-encoded putative N-acetylmuramoyl-L-alanine amidase as a potential antimicrobial to control the bacterium (vol 195, pg 675, 2013) SO ARCHIVES OF MICROBIOLOGY LA English DT Correction C1 [Tillman, Glenn E.; Simmons, Mustafa; Garrish, Johnna K.; Seal, Bruce S.] ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. RP Seal, BS (reprint author), ARS, Poultry Microbiol Safety Res Unit, Richard B Russell Agr Res Ctr, USDA, Athens, GA 30605 USA. EM Bruce.Seal@ars.usda.gov NR 1 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0302-8933 EI 1432-072X J9 ARCH MICROBIOL JI Arch. Microbiol. PD APR PY 2014 VL 196 IS 4 BP 311 EP 311 DI 10.1007/s00203-014-0955-5 PG 1 WC Microbiology SC Microbiology GA AD3BV UT WOS:000333113100010 ER PT J AU Yen, H Wang, XY Fontane, DG Harmel, RD Arabi, M AF Yen, Haw Wang, Xiuying Fontane, Darrell G. Harmel, R. Daren Arabi, Mazdak TI A framework for propagation of uncertainty contributed by parameterization, input data, model structure, and calibration/validation data in watershed modeling SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Model calibration; Error propagation; SWAT model; Bayesian Model Averaging; Uncertainty analysis ID RAINFALL-RUNOFF MODELS; GOODNESS-OF-FIT; SWAT MODEL; ASSESSMENT-TOOL; STREAMFLOW SIMULATION; CATCHMENT MODELS; QUALITY MODELS; PREDICTION; ALGORITHM; BASIN AB Failure to consider major sources of uncertainty may bias model predictions in simulating watershed behavior. A framework entitled the Integrated Parameter Estimation and Uncertainty Analysis Tool (IPEAT), was developed utilizing Bayesian inferences, an input error model and modified goodness-of-fit statistics to incorporate uncertainty in parameter, model structure, input data, and calibration/validation data in watershed modeling. Applications of the framework at the Eagle Creek Watershed in Indiana shows that watershed behavior was more realistically represented when the four uncertainty sources were considered jointly without having to embed watershed behavior constraints in auto-calibration. Accounting for the major sources of uncertainty associated with watershed modeling produces more realistic predictions, improves the quality of calibrated solutions, and consequently reduces predictive uncertainty. IPEAT is an innovative tool to investigate and explore the significance of uncertainty sources, which enhances watershed modeling by improved characterization and assessment of predictive uncertainty. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Yen, Haw; Fontane, Darrell G.; Arabi, Mazdak] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Yen, Haw; Wang, Xiuying] Texas A&M Agrilife Res, Blackland Res & Extens Ctr, Temple, TX 76502 USA. [Harmel, R. Daren] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. RP Yen, H (reprint author), Texas A&M Univ Syst, Texas A&M Agrilife Res, Blackland Res & Extens Ctr, 720 East Blackland Rd, Temple, TX 76502 USA. EM hyen@brc.tamus.edu RI Yen, Haw/K-6809-2014 NR 67 TC 38 Z9 38 U1 9 U2 42 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD APR PY 2014 VL 54 BP 211 EP 221 DI 10.1016/j.envsoft.2014.01.004 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA AC1PA UT WOS:000332267300016 ER PT J AU Kakar, KU Duan, YP Nawaz, Z Sun, GC Almoneafy, AA Hassan, MA Elshakh, A Li, B Xie, GL AF Kakar, Kaleem Ullah Duan, Y. -P. Nawaz, Zarqa Sun, Guochang Almoneafy, Abdlwareth A. Hassan, M. Auwal Elshakh, Ahmed Li, Bin Xie, Guan-Lin TI A novel rhizobacterium Bk7 for biological control of brown sheath rot of rice caused by Pseudomonas fuscovaginae and its mode of action SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Antimicrobial peptide coding genes; Biofilm formation; Brown sheath rot of rice; Multiplex PCR; RT-qPCR ID GROWTH-PROMOTING RHIZOBACTERIA; PLANT-GROWTH; BACILLUS-SUBTILIS; BIOFILM FORMATION; GENE-EXPRESSION; RHIZOCTONIA-SOLANI; BIOCONTROL AGENT; BACTERIA; AMYLOLIQUEFACIENS; SURFACTIN AB The study presents the first report on biocontrol of brown sheath rot disease of rice caused by Pseudomonas fuscovaginae using rhizo-bacterial isolate Bacillus amyloliquefaciens Bk7. Four potential bioactive antagonists were selected from 120 Bacillus isolates. Results obtained from in vitro laboratory assay showed that rhizosphere bacterial strain Bk7 and its metabolites significantly suppressed the growth of Pseudomonas fuscovaginae with 93 % efficacy. In glasshouse experiments, strain Bk7 exhibited biocontrol efficacy of 76.6 % by reducing the disease incidence to 16.9 %, compared to 72.8 % observed in control treatment. In addition, the isolate Bk7 showed the growth promotion efficacy of plant height (GPE, 46.4 %) and fresh weight (GPE, 84.3 %). Characterization of isolate Bk7 revealed its strong capability for biofilm formation, inorganic phosphate solubilization and production of high amounts of Indole-3 acetic acid, siderophores and ammonia in vitro. Results obtained from multiplex PCR assay confirmed the presence of five lipopeptide biosynthetic gene markers (srfAA, fenD, bmyB, bacA and ituC) in the genome of strain Bk7. Moreover, Real-time qPCR of these genes demonstrated that surfactin, iturin and bacylisin coding genes were highly expressed in response to P. fuscovaginae exposure in vitro. Rhizosphere bacterial strain Bk7 was identified as B. amyloliquefaciens strain Bk7 based on the analysis of 16S rDNA internal transcribed spacer sequences and a fatty acid methyl ester analysis. The results obtained from this study showed the potential usefulness of Bk7 as a biocontrol agent in disease control of rice brown sheath rot. C1 [Kakar, Kaleem Ullah; Almoneafy, Abdlwareth A.; Elshakh, Ahmed; Li, Bin; Xie, Guan-Lin] Zhejiang Univ, State Key Lab Rice Biol, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China. [Kakar, Kaleem Ullah; Almoneafy, Abdlwareth A.; Elshakh, Ahmed; Li, Bin; Xie, Guan-Lin] Zhejiang Univ, Key Lab Mol Biol Crop Pathogens & Insects, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China. [Duan, Y. -P.] USDA ARS USHRL, Ft Pierce, FL 34945 USA. [Nawaz, Zarqa] Zhejiang Univ, Key Lab Nucl Agr Sci, Minist Agr & Zhejiang Prov, Inst Nucl Agr Sci, Hangzhou 310029, Zhejiang, Peoples R China. [Sun, Guochang] Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China. [Hassan, M. Auwal] Zhejiang Univ, Coll Agr & Biotechnol, Inst Biotechnol, Lab Plant Nematol, Hangzhou 310058, Zhejiang, Peoples R China. RP Xie, GL (reprint author), Zhejiang Univ, State Key Lab Rice Biol, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China. EM glxie@zju.edu.cn FU Special Fund for Agro-scientific Research in the Public Interest [201303015]; Zhejiang Provincial Natural Science Foundation of China [LY12C14007, R13C140001]; National Natural Science Foundation of China [31371904]; Fundamental Research Funds for the Central Universities; Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control [2010DS700124-KF1101, -KF1203, -KF1309] FX This project was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303015), Zhejiang Provincial Natural Science Foundation of China (LY12C14007), National Natural Science Foundation of China (31371904), Zhejiang Provincial Natural Science Foundation of China (R13C140001), the Fundamental Research Funds for the Central Universities, and Key Subject Construction Program of Zhejiang for Modern Agricultural Biotechnology and Crop Disease Control (2010DS700124-KF1101, -KF1203, -KF1309). NR 77 TC 3 Z9 3 U1 2 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD APR PY 2014 VL 138 IS 4 BP 819 EP 834 DI 10.1007/s10658-013-0356-7 PG 16 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AC7WB UT WOS:000332742500013 ER PT J AU Berentsen, AR Miller, RS Misiewicz, R Malmberg, JL Dunbar, MR AF Berentsen, Are R. Miller, Ryan S. Misiewicz, Regina Malmberg, Jennifer L. Dunbar, Mike R. TI Characteristics of white-tailed deer visits to cattle farms: implications for disease transmission at the wildlife-livestock interface SO EUROPEAN JOURNAL OF WILDLIFE RESEARCH LA English DT Article DE Bovine tuberculosis; Cattle; Farm; Interface; Livestock; Michigan; Mycobacterium bovis; Odocoileus virginianus; White-tailed deer; Wildlife ID MYCOBACTERIUM-BOVIS INFECTION; MICHIGAN WILDLIFE; NEW-ZEALAND; TUBERCULOSIS; CONTACT; MANAGEMENT; BADGERS; EPIDEMIOLOGY; POPULATION; PREVALENCE AB Bovine tuberculosis (bTB) is endemic in free-ranging white-tailed deer (Odocoileus virginianus) in MI, USA. Currently, the rates of farm visitation by deer and co-use of forage resources by cattle and deer are poorly understood. To evaluate the extent deer and livestock may share forage resources, we investigated farm, yard, and cattle-use area visitation by white-tailed deer and compared visitation with common livestock management practices. We fitted 25 female white-tailed deer near the bTB-infected zone in Michigan's Lower Peninsula with global positioning system collars. Livestock management practices associated with farm visitation included presence of confined feeding pastures, number of cattle water sources, and the number of cattle pastures. Fewer farm visits occurred at night than during the day. A higher proportion of nighttime visits occurred between midnight and sunrise. Visitation to yards and cattle-use areas were similar: a higher proportion of visits occurred at night, and a higher proportion of nighttime visits occurred between midnight and sunrise. Multiple visits during the same day were common. Visitation increased through spring and peaked during the fawning season. Results suggest that mitigation and control efforts to guard against potential transmission of bTB should include the season and time of day during which deer visitation occurs. Furthermore, specific livestock management practices may contribute to farm visitation by deer. Deer visiting multiple farms may contribute to local area spread of bTB. Focusing risk mitigation efforts on individual deer that are most likely to visit farms may reduce potential bTB transmission. C1 [Berentsen, Are R.; Misiewicz, Regina; Dunbar, Mike R.] Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Miller, Ryan S.; Malmberg, Jennifer L.] Vet Serv, USDA, APHIS, Ctr Epidemiol & Anim Hlth, Ft Collins, CO 80526 USA. RP Berentsen, AR (reprint author), Wildlife Serv, USDA, APHIS, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM Are.R.Berentsen@aphis.usda.gov; ryan.s.miller@aphis.usda.gov OI Miller, Ryan/0000-0003-3892-0251 FU USDA/APHIS/Veterinary Services FX The authors wish to thank the following: USDA/Wildlife Services, Gaylord, Michigan for logistic support and office space and Minnesota Department of Natural Resources for trapping supplies. Thanks to C. Fitzpatrick, T. Fry, S. Johnson, D. Kilpatrick, R. Pleszewski, and T. Riley for trapping assistance. We are grateful to P. Riggs for data manipulation. Special thanks to M. Farnsworth for consistent input, critical comment, and intellectually stimulating conversation. Finally, a special note of thanks is extended to the private landowners who cooperated in this study. Funding was provided by USDA/APHIS/Veterinary Services. Capture and handling of deer was approved by the USDA/APHIS/WS/National Wildlife Research Center's Institutional Animal Care and Use Committee under protocol QA-1343. NR 57 TC 5 Z9 5 U1 4 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4642 EI 1439-0574 J9 EUR J WILDLIFE RES JI Eur. J. Wildl. Res. PD APR PY 2014 VL 60 IS 2 BP 161 EP 170 DI 10.1007/s10344-013-0760-5 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AC8CD UT WOS:000332759700002 ER PT J AU Palmer, MV Stafne, MR Waters, WR Thacker, TC Phillips, GE AF Palmer, M. V. Stafne, M. R. Waters, W. R. Thacker, T. C. Phillips, G. E. TI Testing a molasses-based bait for oral vaccination of white-tailed deer (Odocoileus virginianus) against Mycobacterium bovis SO EUROPEAN JOURNAL OF WILDLIFE RESEARCH LA English DT Article DE Bait; White-tailed deer; Mycobacterium bovis; Tuberculosis; Vaccination ID BACILLUS-CALMETTE-GUERIN; RABIES VACCINE; TUBERCULOSIS; PROTECTION; WILDLIFE; DANISH; BCG; MANAGEMENT; INFECTION; PATHOLOGY AB White-tailed deer (Odocoileus virginianus) in Michigan, USA, are wildlife reservoirs of bovine tuberculosis (bTB) with documented spread to cattle. In vaccine efficacy trials, Mycobacterium bovis bacillus Calmette-Guerin (BCG) administered orally reduces colonization and bTB-associated lesions in white-tailed deer after experimental challenge with virulent M. bovis. The objective of this study was to develop and evaluate the palatability of a molasses-based bait for oral delivery of BCG to white-tailed deer. Relevant practical properties of the bait such as physical stability under various environmental conditions were evaluated, as well as palatability. Captive deer consumed baits within 3 h of introduction during 48 of 50 trials. Digital game cameras revealed consumption of all placed baits by one deer over 62 % of the time. Addition of BCG vaccine did not negatively impact palatability. Physical stability analysis demonstrated that ice and water significantly reduced bait stability as measured with a compression assay. Storage of BCG-containing baits at 4 degrees C showed a slight decrease in colony-forming units (CFUs) by day 31. In contrast, storage at -20 or -80 degrees C over the same 31-day period showed no significant decrease in BCG viability. The results of this study suggest that molasses-based baits, as prepared here, represent a plausible means of oral delivery of BCG to white-tailed deer under most environmental conditions. C1 [Palmer, M. V.; Stafne, M. R.; Waters, W. R.; Thacker, T. C.] USDA, Natl Anim Dis Ctr, Agr Res Serv, Ames, IA 50010 USA. [Phillips, G. E.] Natl Wildlife Res Ctr, USDA, Ft Collins, CO USA. RP Palmer, MV (reprint author), USDA, Natl Anim Dis Ctr, Agr Res Serv, 1920 Dayton Ave, Ames, IA 50010 USA. EM mitchell.palmer@ars.usda.gov NR 26 TC 3 Z9 3 U1 1 U2 10 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4642 EI 1439-0574 J9 EUR J WILDLIFE RES JI Eur. J. Wildl. Res. PD APR PY 2014 VL 60 IS 2 BP 265 EP 270 DI 10.1007/s10344-013-0777-9 PG 6 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AC8CD UT WOS:000332759700013 ER PT J AU Irvine, RJ Moseley, MH Leckie, F Martinez-Padilla, J Donley, D Miller, A Pound, M Mougeot, F AF Irvine, R. J. Moseley, M. H. Leckie, F. Martinez-Padilla, J. Donley, D. Miller, A. Pound, M. Mougeot, F. TI Investigating the loss of recruitment potential in red grouse (Lagopus lagopus scoticus): the relative importance of hen mortality, food supply, tick infestation and louping-ill SO EUROPEAN JOURNAL OF WILDLIFE RESEARCH LA English DT Article DE Food supply; Louping-ill virus; Mortality; Recruitment; Red grouse; Ticks ID VIRUS FLAVIVIRUS GROUP; MOUNTAIN HARES; EXPERIMENTAL INFECTION; RAPTOR PREDATION; IXODES-RICINUS; RT-PCR; DISEASE; CHICKS; MAMMALS; PERSISTENCE AB Ticks and their pathogens cause significant disease and economic loss in many animal populations. Despite this, experiments that test the impact of ticks and tick-borne diseases on wild animal populations are rare. Here, we report on an experiment assessing the effect of ticks on red grouse productivity and chick growth in relation to other causes of poor recruitment at two sites in the Scottish uplands during 2005. Treated hens received two leg bands impregnated with the acaricide permethrin, while controls hens were untreated. Chicks were captured at c.2 weeks of age and fitted with a metal patagial tag, and chicks from treated hens also received a permethrin-impregnated strip. Mean tick burdens in treated chicks were close to zero compared with a mean of around 12 in the control group. Although treatment reduced tick infestations, it did not increase brood size. Growth rates in chicks from control and treated hens were similar during the first 10 days and comparable with chicks fed an ad-lib invertebrate-based diet. These results suggest that in this case, neither ticks (and the tick transmitted louping-ill virus) nor food shortages was the main cause of chick mortality. However, mortality in the adult hens was around 35 %, and predation accounted for 62 % of these losses before broods fledged. Our results indicate that on our study sites, predation may have a more important impact on grouse population dynamics than ticks and tick-borne disease. We suggest that it may be more cost effective to determine the causes of poor grouse population performance before implementing popular but expensive tick control measures such as the culling of alternative hosts and running acaracide treated sheep 'tick-mop' flocks. C1 [Irvine, R. J.] James Hutton Inst, Aberdeen AB15 8QH, Scotland. [Moseley, M. H.] Dukes Vet Practice, Aboyne AB34 5JH, Aberdeen, Scotland. [Moseley, M. H.] Univ Pretoria, Ctr Wildlife Management, ZA-0028 Pretoria, South Africa. [Leckie, F.] Nat Res Projects Ltd, Banchory AB314BY, Kincardine, Scotland. [Martinez-Padilla, J.] CSIC, Museo Nacl Ciencias Nat, Dept Evolutionary Ecol, E-28006 Madrid, Spain. [Donley, D.] Head Keepers House, Edzell DD9 7UP, Angus, Scotland. [Miller, A.; Pound, M.] USDA ARS, Knipling Bushland US Livestock Insects Res La, Kerrville, TX 78028 USA. [Mougeot, F.] UCLM, JCCM, Inst Invest Recursos Cineget IREC CSIC, Ciudad Real 13005, Spain. [Mougeot, F.] CSIC, Estac Expt Zonas Aridas EEZA, La Canada De San Urbano, Almeria, Spain. RP Irvine, RJ (reprint author), James Hutton Inst, Aberdeen AB15 8QH, Scotland. EM justin.irvine@hutton.ac.uk RI Martinez-Padilla, Jesus/C-6446-2008; Irvine, Robert/G-5074-2012; Evolutionary Ecology, Ecologia Evolutiva/M-3553-2014; Mougeot, Francois/B-1894-2009 OI Martinez-Padilla, Jesus/0000-0003-2956-5163; Irvine, Robert/0000-0002-0411-6217; Mougeot, Francois/0000-0002-5819-6275 FU Royal College of Veterinary Surgeons' Trust FX This project was co-funded by Marquis of Lansdowne, Henry Keswick, Charles Pearson and Charles Gladstone. MM received a grant from the Royal College of Veterinary Surgeons' Trust. LIV seroprevalence was provided by the Moredun Research Institute. Young chicks were located by Coll and Tiree. The project relied heavily on the preparatory work of DD. The field work was carried out whilst RJI, FL, JMP and FRM were affiliated to the Centre for Ecology & Hydrology, and MM was registered at the University of Pretoria, 0028, South Africa NR 40 TC 0 Z9 0 U1 1 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4642 EI 1439-0574 J9 EUR J WILDLIFE RES JI Eur. J. Wildl. Res. PD APR PY 2014 VL 60 IS 2 BP 313 EP 322 DI 10.1007/s10344-013-0788-6 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AC8CD UT WOS:000332759700019 ER PT J AU Sitther, V Zhang, D Harris, DL Yadav, AK Zee, FT Meinhardt, LW Dhekney, SA AF Sitther, V. Zhang, D. Harris, D. L. Yadav, A. K. Zee, F. T. Meinhardt, L. W. Dhekney, S. A. TI Genetic characterization of guava (Psidium guajava L.) germplasm in the United States using microsatellite markers SO GENETIC RESOURCES AND CROP EVOLUTION LA English DT Article DE Cultivar identification; Fingerprinting; Genetic variation; Homozygosity; Inbreeding; Molecular markers; Psidium guajava; Simple sequence repeat ID POPULATION-STRUCTURE; SOFTWARE AB Genetic diversity of 35 Psidium guajava L. accessions and three related species (P. guineense Sw., P. sartorianum (O. Berg) Nied. and P. friedrichsthalianum (O. Berg) Nied.) maintained at the U.S. Department of Agriculture (USDA), National Plants Germplasm System, Hilo, HI, was characterized using 20 simple sequence repeat (SSR) markers. Diversity analysis detected a total of 178 alleles ranging from 4 to 16 alleles per locus. The observed mean heterozygosity (0.2) and inbreeding coefficient (0.8) indicated a high level of inbreeding among the accessions tested. Multi-locus DNA fingerprints based on the 20 SSR loci unambiguously differentiated all accessions and revealed the absence of duplicated samples. Ordination and cluster analyses suggested that the genetic relationships between majorities of the accessions could be explained by geographic origin, mainly including tropical America, Southeast Asia and Hawaii. A Bayesian cluster analysis partitioned the accessions into two groups and the partition was largely compatible with the result of ordination analyses. Distance-based cluster analyses further indicated that accessions from same geographical region or breeding programs grouped together in spite of the inter-regional exchange of germplasm. Accessions from Southeast Asia were dominantly white fleshed, whereas accessions from tropical America and Hawaii exhibited diverse flesh colors. The results also indicated that accessions from the same region were likely derived from a small number of common ancestors or progenitors. All 20 SSRs were transferable to P. guineense, P. sartorianum and P. friedrichsthalianum, indicating a close relationship between the cultigens and wild relatives. Application of SSR markers in the USDA/Agricultural Research Service germplasm collection provides new insight into the diversity of the guava germplasm, which will be valuable in future breeding endeavors and the conservation of guava genetic resources. C1 [Sitther, V.] Morgan State Univ, Dept Biol, Baltimore, MD 21251 USA. [Zhang, D.; Meinhardt, L. W.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Harris, D. L.] St Martins Univ, Lacey, WA 98503 USA. [Yadav, A. K.] Ft Valley State Univ, Agr Res Stn, Ft Valley, GA 31030 USA. [Zee, F. T.] Trop Plant Genet Resources & Dis Res, Hilo, HI 96720 USA. [Dhekney, S. A.] Univ Wyoming, Sheridan Res & Extens Ctr, Sheridan, WY 82801 USA. RP Sitther, V (reprint author), Morgan State Univ, Dept Biol, 1700 E Cold Spring Lane, Baltimore, MD 21251 USA. EM viji.sitther@morgan.edu FU USDA-CSREES [2004-38814-15128] FX Financial support of the USDA-CSREES through an award Agreement 2004-38814-15128 to The Fort Valley State University is greatly appreciated. NR 27 TC 5 Z9 5 U1 2 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0925-9864 EI 1573-5109 J9 GENET RESOUR CROP EV JI Genet. Resour. Crop Evol. PD APR PY 2014 VL 61 IS 4 BP 829 EP 839 DI 10.1007/s10722-014-0078-5 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AC9MQ UT WOS:000332859100009 ER PT J AU Katzner, TE Jackson, DS Ivy, J Bragin, EA DeWoody, A AF Katzner, Todd E. Jackson, Daniel S. Ivy, Jamie Bragin, Evgeny A. DeWoody, Andrew TI Variation in offspring sex ratio of a long-lived sexually dimorphic raptor, the Eastern Imperial Eagle Aquila heliaca SO IBIS LA English DT Article DE Aquila heliaca; brood sex ratio; eagle; irregular variation; stochastic variation ID KESTREL FALCO-NAUMANNI; LESSER KESTREL; PARENTAL INVESTMENT; TAWNY OWLS; ALLOCATION; BIRDS; MANIPULATION; KAZAKSTAN; POPULATION; ADJUSTMENT AB Sex ratio theory attempts to explain observed variation in offspring sex ratio at both the population and the brood levels. In the context of low-fecundity organisms producing high-investment offspring, the drivers of adaptive variation in sex ratio are incompletely understood. For raptors that display reverse sexual dimorphism (RSD), preferential allocation of resources to the putatively cheaper sex (male) may be a response to environmental, social or demographic stressors. To assess the extent of skew in offspring sex ratios and to evaluate possible dietary, environmental and demographic correlates of such skew to long-lived RSD avian species, we evaluated the offspring sex ratio of 219 chicks from 119 broods in 30 territories of Eastern Imperial Eagles Aquila heliaca across 7years and four regions at a nature reserve in Kazakhstan. Only in one region in 1year of our study did the offspring sex ratio differ significantly from parity (10 males : 1 female in 11 territories). Whereas offspring sex ratios were independent of dietary diversity, precipitation, temperature and productivity, we found that year had a moderate effect on brood sex ratio within territories. Our results provide limited evidence of brood sex manipulation in these populations of Eastern Imperial Eagles, and no mechanistic insight into predictions associated with it. Stochastic variation is likely to explain much of the fluctuation we observed in sex ratios, but our observations are also consistent with the hypothesis that sex-ratio manipulation may occur irregularly, in concurrence with atypical environmental or demographic conditions that fluctuate at a time scale longer than that of our 7-year study. C1 [Katzner, Todd E.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Katzner, Todd E.] US Forest Serv, USDA, No Res Stn, Parsons, WV USA. [Jackson, Daniel S.] Oberlin Coll, Dept Biol, Oberlin, OH 44074 USA. [Ivy, Jamie; DeWoody, Andrew] Purdue Univ, Dept Forestry & Nat Resources, W Lafayette, IN 47907 USA. [Bragin, Evgeny A.] Naurzum Natl Nat Reserve, Dept Sci, Naurzumski Raijon, Karamendy, Kazakhstan. RP Katzner, TE (reprint author), W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. EM todd.katzner@mail.wvu.edu OI Katzner, Todd/0000-0003-4503-8435 FU Wildlife Conservation Society; National Birds of Prey Trust; US National Science Foundation [INT-0301905]; US Environmental Protection Agency; USGS Biological Resources Division; Arizona State University Department of Biology; Frank M. Chapman Memorial Fund; Hawk Mountain-Zeiss Raptor Research Award; Arizona State University Graduate Research Support Program; International Osprey Foundation; World Nature Association; National Geographic Society FX Steve Latta, Vincenzo Penteriani, Gary Bortolotti, Patrik Byholm, Beatriz Arroyo, Rauri Bowie and several anonymous reviewers provided insightful comments on the manuscripts. Adam Duerr assisted with analysis. This project was funded by the Wildlife Conservation Society, the National Birds of Prey Trust, the US National Science Foundation (INT-0301905), the US Environmental Protection Agency, the USGS Biological Resources Division, the Arizona State University Department of Biology, the Frank M. Chapman Memorial Fund, the Hawk Mountain-Zeiss Raptor Research Award, the Arizona State University Graduate Research Support Program, the International Osprey Foundation, the World Nature Association and the National Geographic Society. West Virginia University, the National Aviary, Purdue University, the Naurzum National Nature Reserve and the government of Kazakhstan provided critical logistical, permitting and institutional support. NR 55 TC 1 Z9 1 U1 2 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0019-1019 EI 1474-919X J9 IBIS JI Ibis PD APR PY 2014 VL 156 IS 2 BP 395 EP 403 DI 10.1111/ibi.12139 PG 9 WC Ornithology SC Zoology GA AC8OK UT WOS:000332792800013 ER PT J AU Fayer, R Elsasser, T Gould, R Solano, G Urban, J Santin, M AF Fayer, R. Elsasser, T. Gould, R. Solano, G. Urban, J., Jr. Santin, M. TI Blastocystis tropism in the pig intestine SO PARASITOLOGY RESEARCH LA English DT Article ID HOMINIS INFECTION; ZOO ANIMALS; CYSTEINE PROTEASES; GENETIC DIVERSITY; IN-VITRO; LIVESTOCK; PARASITE AB Blastocystis has been reported in pig feces but the sites of development in the gastrointestinal tract are unknown. The present study was undertaken to determine predilection sites of Blastocystis in 11 naturally infected pigs examined at 20 weeks of age. At necropsy, feces and contents of the duodenum, jejunum, ileum, and cecum were examined by immunofluorescence (IFA) microscopy and PCR and tissues from these sites as well as the proximal and distal colon were processed for histology from pigs 1 to 5. Feces were examined by IFA microscopy, and segments from the jejunum and ileum were processed for histology from pigs 6 to 11. Multiple sections were cut from each tissue segment, and each was stained with the following: hematoxylin and eosin, polyclonal rabbit antibody to Blastocystis, and ParaFlor B (TM) monoclonal antibody to Blastocystis. Blastocystis was detected in feces of all 11 pigs by IFA microscopy and determined by PCR and gene sequencing to be subtype 5 for pigs 1-5. Blastocystis was also detected in the lumen contents removed from the cecum of pigs 1-5 examined by IFA microscopy and in the cecum of pigs 4 and 5 by PCR. Blastocystis was also observed in tissue sections from the jejunum of 7 of the 11 pigs, in the proximal and distal colon of pigs 1-5, and in the cecum of 4 of these 5 pigs but was not detected in the duodenum or ileum of any pigs. In tissue sections, Blastocystis was found primarily in the lumen usually associated with digested food debris, sometimes in close proximity or appearing to adhere to the epithelium, but no stages were found to penetrate the epithelium or the lamina propria. C1 [Fayer, R.; Elsasser, T.; Santin, M.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Gould, R.] Antibodies Inc, Davis, CA 95617 USA. [Solano, G.; Urban, J., Jr.] ARS, USDA, Ctr Human Nutr, Beltsville, MD 20705 USA. RP Fayer, R (reprint author), ARS, USDA, Beltsville Agr Res Ctr, 10300 Baltimore Ave,Bldg 173, Beltsville, MD 20705 USA. EM ronald.fayer@ars.usda.gov NR 35 TC 7 Z9 7 U1 0 U2 6 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0932-0113 EI 1432-1955 J9 PARASITOL RES JI Parasitol. Res. PD APR PY 2014 VL 113 IS 4 BP 1465 EP 1472 DI 10.1007/s00436-014-3787-y PG 8 WC Parasitology SC Parasitology GA AD1YO UT WOS:000333029800024 PM 24535732 ER PT J AU Shao, M Kumar, S Thomson, JG AF Shao, Min Kumar, Shashi Thomson, James G. TI Precise excision of plastid DNA by the large serine recombinase Bxb1 SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE Nicotiana tabacum; chloroplast transformation; Bxb1 recombinase; site-specific recombination; transgene excision ID SITE-SPECIFIC RECOMBINASE; MYCOBACTERIOPHAGE BXB1; TOBACCO PLASTIDS; TRANSGENIC WHEAT; GENE-TRANSFER; HIGHER-PLANTS; MARKER; EXPRESSION; GENOME; TRANSFORMATION AB Marker genes are essential for the selection and identification of rarely occurring transformation events generated in biotechnology. This includes plastid transformation, which requires that multiple copies of the modified chloroplast genome be present to obtain genetically stable transplastomic plants. However, the marker gene becomes dispensable when homoplastomic plants are obtained. Here, we demonstrate the precise excision of attP- and attB-flanked DNA from the plastid genome mediated by the large serine recombinase Bxb1. We transformed the tobacco plastid genome with the pTCH-PB vector containing a stuffer fragment of DNA flanked by directly oriented nonhomologous attP and attB recombinase recognition sites. In the absence of the Bxb1 recombinase, the transformed plastid genomes were stable and heritable. Nuclear-transformed transgenic tobacco plants expressing a plastid-targeted Bxb1 recombinase were crossed with transplastomic pTCH-PB plants, and the T-1 hybrids exhibited efficient excision of the target sequence. The Bxb1-att system should prove to be a useful tool for site-specifically manipulating the plastid genome and generating marker-free transplastomic plants. C1 [Shao, Min] Univ Calif Davis, Dept Plant Sci, Davis, CA USA. [Kumar, Shashi] Int Ctr Genet Engn & Biotechnol, New Delhi, India. [Thomson, James G.] USDA ARS, Western Reg Res Ctr, Crop Improvement & Utilizat Res Unit, Albany, CA 94710 USA. RP Thomson, JG (reprint author), USDA ARS, Western Reg Res Ctr, Crop Improvement & Utilizat Res Unit, 800 Buchanan St, Albany, CA 94710 USA. EM james.thomson@ars.usda.gov FU USDA-ARS [5325-21000-018-00D] FX We express our thanks to Ron Chan and Bryan Hernandez for technical assistance. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Research was funded by USDA-ARS project 5325-21000-018-00D. USDA is an equal opportunity provider and employer. NR 37 TC 5 Z9 6 U1 0 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD APR PY 2014 VL 12 IS 3 BP 322 EP 329 DI 10.1111/pbi.12139 PG 8 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA AD1XR UT WOS:000333027500005 PM 24261912 ER PT J AU Beckstead, J Meyer, SE Reinhart, KO Bergen, KM Holden, SR Boekweg, HF AF Beckstead, Julie Meyer, Susan E. Reinhart, Kurt O. Bergen, Kellene M. Holden, Sandra R. Boekweg, Heather F. TI Factors affecting host range in a generalist seed pathogen of semi-arid shrublands SO PLANT ECOLOGY LA English DT Article DE Drechslera campanulata; Germination rate; Pathogen resistance; Pathogen tolerance; Phylogenetic distance; Plant-microbe interactions ID BROMUS-TECTORUM; PYRENOPHORA-SEMENIPERDA; GENETIC-VARIATION; BANK PATHOGEN; VIRULENCE; FUNGI; CONSEQUENCES; ENVIRONMENT; DYNAMICS; PLANTS AB Generalist pathogens can exhibit differential success on different hosts, resulting in complex host range patterns. Several factors operate to reduce realized host range relative to potential host range, particularly under field conditions. We explored factors influencing host range of the naturally occurring generalist ascomycete grass seed pathogen Pyrenophora semeniperda. We measured potential host range in laboratory experiments at high inoculum loads with 26 grass species, including the primary host Bromus tectorum, and developed models to predict susceptibility and tolerance based on host traits, including germination speed, seed hardness, seed size, and phylogenetic relations. We also examined pathogen and host density effects on infection and mortality. All species tested were at least somewhat susceptible to the pathogen at high inoculum loads, but both infection and mortality varied widely. Species more closely related to the original host (B. tectorum) were more susceptible to infection, whereas species with slower germination were less tolerant and therefore more likely to suffer mortality. Infection and mortality were sharply reduced as inoculum load was reduced. Intermediate loads had major negative impacts on dormant B. tectorum seeds but generally minimal effects on native species. In addition, field seed bank studies determined that P. semeniperda rarely exploits native grass species as hosts. This marked reduction in realized host range relative to potential host range indicates that laboratory host range studies are potentially a poor predictor of either the current or possible future realized host range for wildland plant pathogens. C1 [Beckstead, Julie; Bergen, Kellene M.; Holden, Sandra R.] Gonzaga Univ, Dept Biol, Spokane, WA 99258 USA. [Meyer, Susan E.] US Forest Serv, Shrub Sci Lab, USDA, Rocky Mt Res Stn, Provo, UT 84606 USA. [Reinhart, Kurt O.] ARS, Ft Keogh Livestock & Range Res Lab, USDA, Miles City, MT 59301 USA. [Boekweg, Heather F.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA. RP Beckstead, J (reprint author), Gonzaga Univ, Dept Biol, 502 E Boone Ave, Spokane, WA 99258 USA. EM beckstead@gonzaga.edu RI Reinhart, Kurt/A-6730-2010 FU Joint Fire Sciences Program [JFSP-2007-1-3-10]; CSREES NRI Biology of Weedy and Invasive Species Program [2008-35320-18677]; Howard Hughes Medical Institute through the Undergraduate Science Education Program; Gonzaga University FX We thank Hanford Reach National Monument WA, Washington Dept Natural Resources, Hugh Lefcort of Spokane WA, and the Bureau of Land Management (BLM) Salt Lake City UT District for access to field sites. We thank Glenn Paulson, BLM Spokane WA, Karen Prentice BLM Ely NV, and Kathleen Harcksen, Grand Canyon Parashant National Monument AZ for providing seed. Our thanks to Ingrid Velez, Katherine Horlick, June Jung, Laura Street, Trevor Davis, Abbey Shuster, Kaitlin Van Volkom, Katie Merrill, Keith Merrill, Thomas Stewart, Steven Harrison, Kedra Foote, and Phil Allen, whose technical help made this project possible. Thanks to Suzette Clement for inoculum production. Research was funded in part by the Joint Fire Sciences Program (JFSP-2007-1-3-10; J.B., S.E.M.), the CSREES NRI Biology of Weedy and Invasive Species Program (2008-35320-18677; J.B., S.E.M.). This research was supported in part by a grant to Gonzaga University from the Howard Hughes Medical Institute through the Undergraduate Science Education Program and from the Claire and Robert McDonald Work Award Program from Gonzaga University (J.B.). NR 28 TC 5 Z9 5 U1 1 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-0237 EI 1573-5052 J9 PLANT ECOL JI Plant Ecol. PD APR PY 2014 VL 215 IS 4 BP 427 EP 440 DI 10.1007/s11258-014-0313-3 PG 14 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA AD2GN UT WOS:000333052400006 ER PT J AU Simko, I Rauscher, G Sideman, RG McCreight, JD Hayes, RJ AF Simko, I. Rauscher, G. Sideman, R. G. McCreight, J. D. Hayes, R. J. TI Evaluation and QTL mapping of resistance to powdery mildew in lettuce SO PLANT PATHOLOGY LA English DT Article DE aggregate-ranking; area under the disease progress steps; field resistance; Golovinomyces cichoracearum; greenhouse resistance; Lactuca sativa ID LACTUCA-SATIVA L.; ERYSIPHE-CICHORACEARUM; NATURAL INFECTION; DISEASE RESISTANCE; NONHOST RESISTANCE; DOWNY MILDEW; SSR MARKERS; CULTIVARS; SUSCEPTIBILITY; ARCHITECTURE AB Lettuce (Lactuca sativa) is the major leafy vegetable that is susceptible to powdery mildew disease under greenhouse and field conditions. Quantitative trait loci (QTLs) for resistance to powdery mildew under greenhouse conditions were mapped in an interspecific population derived from a cross between susceptible L.sativa cultivar Salinas and the highly susceptible L.serriola accession UC96US23. Four significant QTLs were detected on linkage groups LG 1 (pm-1.1), LG 2 (pm-2.1 and pm-2.2) and LG 7 (pm-7.1), each explaining between 35 to 42% of the phenotypic variation. The four QTLs are not located in the documented hotspots of lettuce resistance genes. Alleles for the disease resistance at the four QTLs originated from both parents (two from each), demonstrating that even highly susceptible accessions may provide alleles for resistance to powdery mildew. These QTLs appeared to operate during limited periods of time. Results of the field trials with F-2:3 and F-3:4 families derived from a Soraya (moderately resistant)xSalinas cross demonstrated effective transfer of resistance to powdery mildew in this material. An integrated rating approach was used to estimate relative levels of resistance in 80 cultivars and accessions tested in a total of 23 field and greenhouse experiments. Generally, very low resistance was observed in crisphead-type lettuces, while the highest relative resistance was recorded in leaf and butterhead types. Comparison of two disease assessment methods (percentage rating and the area under the disease progress steps, AUDPS) for detection of QTLs shows that the two approaches complement each other. C1 [Simko, I.; Rauscher, G.; Sideman, R. G.; McCreight, J. D.; Hayes, R. J.] ARS, USDA, US Agr Res Stn, Salinas, CA 93905 USA. RP Simko, I (reprint author), ARS, USDA, US Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM ivan.simko@ars.usda.gov RI Simko, Ivan/J-6048-2012 OI Simko, Ivan/0000-0002-8769-8477 FU California Leafy Greens Research Program; Arizona Iceberg Lettuce Research Council FX The authors would like to thank R. Michelmore, M. Truco, O. Ochoa and L. McHale for providing marker information and seeds of the mapping population. This work was partly supported by the California Leafy Greens Research Program and the Arizona Iceberg Lettuce Research Council. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 35 TC 3 Z9 3 U1 1 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD APR PY 2014 VL 63 IS 2 BP 344 EP 353 DI 10.1111/ppa.12087 PG 10 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AC8BK UT WOS:000332757800011 ER PT J AU Arias, RS Sobolev, VS Orner, VA Dang, PM Lamb, MC AF Arias, R. S. Sobolev, V. S. Orner, V. A. Dang, P. M. Lamb, M. C. TI Potential involvement of Aspergillus flavus laccases in peanut invasion at low water potential SO PLANT PATHOLOGY LA English DT Article DE aflatoxin; Arachis hypogaea; drought; groundnut; phytoalexins; stilbenoids ID REAL-TIME PCR; DROUGHT-STRESSED PEANUTS; RIBOSOMAL-RNA SYNTHESIS; ARACHIS-HYPOGAEA SEEDS; AFLATOXIN CONTAMINATION; STILBENE PHYTOALEXINS; FUNGAL LACCASES; GENE-EXPRESSION; MATURITY; INTERRELATIONSHIP AB Aspergillus flavus accumulates carcinogenic aflatoxins in peanuts, mainly in immature kernels during drought. Aspergillus flavus invasion induces accumulation of phytoalexins, mostly stilbenoids in peanut, as a plant defence mechanism. Because fungal laccases are often related to pathogenicity and can degrade stilbenoids, this study reports for the first time the expression of A.flavus laccases in the presence of kernels, hulls and low water potential in relation to the accumulation of phytoalexins in peanut kernels. Packed-cell volume (PCV) of A.flavus biomass was significantly higher (P001) in the presence of mature kernels, dead kernels, and mature and immature peanut hulls than the control. The presence of kernels and hulls lowered the level of expression of three A.flavus laccases by 4-6-fold (P<001), whereas 3% sucrose up-regulated them by 35-304-fold, and low water potential (-11MPa) up-regulated them by 85-248-fold (P<001). Phytoalexins that accumulated in peanut kernels in the presence of A.flavus and were quantified by HPLC-DAD-MS were primarily the stilbenoids: 3-isopentadienyl-3,5,4-trihydroxystilbene (IPD), chiricanine-A, arachidin-2, arachidin-3 and arahypin-1. Apparent degradation of phytoalexins was observed when using a priori induction of phytoalexins in seeds in combination with a priori induction of laccases in A.flavus. The up-regulation of laccase expression observed at -11MPa and at high sucrose concentration could be contributing to peanut invasion in immature kernels under drought conditions. C1 [Arias, R. S.; Sobolev, V. S.; Orner, V. A.; Dang, P. M.; Lamb, M. C.] ARS, USDA, Natl Peanut Res Lab, Dawson, GA 39842 USA. RP Arias, RS (reprint author), ARS, USDA, Natl Peanut Res Lab, 1011 Forrester Dr SE, Dawson, GA 39842 USA. EM renee.arias@ars.usda.gov FU USDA-ARS [6604-42000-008-00D, 6604-21000-003-00D] FX This work was supported by USDA-ARS project numbers 6604-42000-008-00D and 6604-21000-003-00D. The authors thank Dr Bruce Horn for providing the strain Aspergillus flavus NRRL 3357. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 56 TC 1 Z9 1 U1 2 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-0862 EI 1365-3059 J9 PLANT PATHOL JI Plant Pathol. PD APR PY 2014 VL 63 IS 2 BP 354 EP 364 DI 10.1111/ppa.12088 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AC8BK UT WOS:000332757800012 ER PT J AU Song, LY Zhang, Y Li, SF Hu, J Yin, WB Chen, YH Hao, ST Wang, BL Wang, RRC Hu, ZM AF Song, Li-Ying Zhang, Yan Li, Shu-Fen Hu, Jun Yin, Wei-Bo Chen, Yu-Hong Hao, Shan-Ting Wang, Bai-Lin Wang, Richard R. -C. Hu, Zan-Min TI Identification of the substrate recognition region in the Delta(6)-fatty acid and Delta(8)-sphingolipid desaturase by fusion mutagenesis SO PLANTA LA English DT Article DE Delta(6)-Fatty acid desaturase; Delta(8)-Sphingolipid desaturase; Fusion protein; Substrate recognition; Enzyme activity ID CYTOCHROME B(5) DOMAIN; FATTY-ACIDS; GENE; EVOLUTION; EXPRESSION; PROTEINS; DELTA(6)-DESATURASE; FAMILY; SUBFUNCTIONALIZATION; REGIOSELECTIVITY AB Delta(8)-sphingolipid desaturase and Delta(6)-fatty acid desaturase share high protein sequence identity. Thus, it has been hypothesized that Delta(6)-fatty acid desaturase is derived from Delta(8)-sphingolipid desaturase; however, there is no direct proof. The substrate recognition regions of Delta(6)-fatty acid desaturase and Delta(8)-sphingolipid desaturase, which aid in understanding the evolution of these two enzymes, have not been reported. A blackcurrant Delta(6)-fatty acid desaturase and a Delta(8)-sphingolipid desaturase gene, RnD6C and RnD8A, respectively, share more than 80 % identity in their coding protein sequences. In this study, a set of fusion genes of RnD6C and RnD8A were constructed and expressed in yeast. The Delta(6)- and Delta(8)-desaturase activities of the fusion proteins were characterized. Our results indicated that (1) the exchange of the C-terminal 172 amino acid residues can lead to a significant decrease in both desaturase activities; (2) amino acid residues 114-174, 206-257, and 258-276 played important roles in Delta(6)-substrate recognition, and the last two regions were crucial for Delta(8)-substrate recognition; and (3) amino acid residues 114-276 of Delta(6)-fatty acid desaturase contained the substrate recognition site(s) responsible for discrimination between ceramide (a substrate of Delta(8)-sphingolipid desaturase) and acyl-PC (a substrate of Delta(6)-fatty acid desaturase). Substituting the amino acid residues 114-276 of RnD8A with those of RnD6C resulted in a gain of Delta(6)-desaturase activity in the fusion protein but a loss in Delta(8)-sphingolipid desaturase activity. In conclusion, several regions important for the substrate recognition of Delta 8-sphingolipid desaturase and Delta(6)-fatty acid desaturase were identified, which provide clues in understanding the relationship between the structure and function in desaturases. C1 [Song, Li-Ying; Zhang, Yan; Li, Shu-Fen; Hu, Jun; Yin, Wei-Bo; Chen, Yu-Hong; Hao, Shan-Ting; Hu, Zan-Min] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China. [Li, Shu-Fen] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China. [Wang, Bai-Lin] Heilongjiang Agr Acad, Hort Div, Harbin 150069, Peoples R China. [Wang, Richard R. -C.] Utah State Univ, FRRL, USDA ARS, Logan, UT 84322 USA. RP Hu, ZM (reprint author), Chinese Acad Sci, Inst Genet & Dev Biol, Datun Rd, Beijing 100101, Peoples R China. EM lysong@genetics.ac.cn; mai_xm@hotmail.com; sfli@genetics.ac.cn; jhu@genetics.ac.cn; wbyin@genetics.ac.cn; yhchen@genetics.ac.cn; sthao@genetics.ac.cn; wangbailin1@126.com; richard.wang@ars.usda.gov; zmhu@genetics.ac.cn FU Ministry of Agriculture of China for transgenic research [2011ZX08009-003-004, 2009ZX08009-098B]; Ministry of Science and Technology of China [2011CB200902] FX This research was supported by projects (nos. 2011ZX08009-003-004 and 2009ZX08009-098B) from the Ministry of Agriculture of China for transgenic research, and a project (2011CB200902) from the Ministry of Science and Technology of China. The RP-HPLC analysis was carried out by the Beijing Centre for Physical and Chemical Analysis. NR 39 TC 2 Z9 3 U1 1 U2 17 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD APR PY 2014 VL 239 IS 4 BP 753 EP 763 DI 10.1007/s00425-013-2006-x PG 11 WC Plant Sciences SC Plant Sciences GA AD3FZ UT WOS:000333125000002 PM 24366682 ER PT J AU McDaniel, MD Wagner, RJ Rollinson, CR Kimball, BA Kaye, MW Kaye, JP AF McDaniel, M. D. Wagner, R. J. Rollinson, C. R. Kimball, B. A. Kaye, M. W. Kaye, J. P. TI Microclimate and ecological threshold responses in a warming and wetting experiment following whole tree harvest SO THEORETICAL AND APPLIED CLIMATOLOGY LA English DT Article ID NORTHERN HARDWOOD FOREST/; FREEZE-THAW EVENTS; CLIMATE-CHANGE; SOIL-WATER; FUNCTIONAL-GROUPS; LEAF TEMPERATURE; MESIC GRASSLAND; ECOSYSTEM; CARBON; MANIPULATION AB Ecosystem climate manipulation experiments (ECMEs) are a key tool for predicting the effects of climate change on ecosystems. However, the strength of inferences drawn from these experiments depends on whether the manipulated conditions mimic future climate changes. While ECMEs have examined mean temperature and moisture conditions, ecosystem processes may respond more to microclimatic thresholds (e.g., freeze-thaw events). We reported the mean and microclimatic thresholds from a post-clearcut ECME in a temperate, mixed deciduous forest. Target treatments were ambient, warmed (+similar to 2 A degrees C), wetted (+similar to 20 % precipitation), and warmed + wetted. Wetted treatments increased mean monthly precipitation by 23 %, but did not change the amount of time the soil water potential was below the permanent wilting point. Relative to ambient, warmed treatments increased the mean temperatures of the surface and soil by 1.8 and 2.5 A degrees C, respectively. Warming decreased the number of soil freeze-thaw events and increased the number of growing degree days, frost-free days, and amount of time leaf surface temperatures were in the optimal photosynthetic range. Our results showed that, even when ECMEs mimic mean predicted climate conditions, their effect on microclimatic thresholds can be variable. We suggest that measuring these and other microclimatic thresholds will be essential for interpreting ECME results and assessing their value in predicting ecosystem responses to future climate change. C1 [McDaniel, M. D.] Univ New Hampshire, Dept Nat Resources & Environm, Durham, NH 03824 USA. [Wagner, R. J.; Rollinson, C. R.; Kaye, M. W.; Kaye, J. P.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Kimball, B. A.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP McDaniel, MD (reprint author), Univ New Hampshire, Dept Nat Resources & Environm, 114 James Hall, Durham, NH 03824 USA. EM marshall.mcdaniel@unh.edu OI Rollinson, Christine/0000-0003-0181-7293 FU Northeastern Regional Center of the Department of Energy National Institute for Climate Change Research FX We thank Brandon Frazier, Christopher Hone, and Erica Dreibelbis for their help in the field and laboratory. This research was supported by a grant from the Northeastern Regional Center of the Department of Energy National Institute for Climate Change Research. NR 54 TC 5 Z9 5 U1 3 U2 31 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0177-798X EI 1434-4483 J9 THEOR APPL CLIMATOL JI Theor. Appl. Climatol. PD APR PY 2014 VL 116 IS 1-2 BP 287 EP 299 DI 10.1007/s00704-013-0942-9 PG 13 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA AD3ET UT WOS:000333121800022 ER PT J AU Sharma, P Elder, T Groom, LH Spivey, JJ AF Sharma, Pratibha Elder, Thomas Groom, Leslie H. Spivey, James J. TI Effect of Structural Promoters on Fe-Based Fischer-Tropsch Synthesis of Biomass Derived Syngas SO TOPICS IN CATALYSIS LA English DT Article DE Biomass syngas; Fischer-Tropsch synthesis; Fe based catalysts; Structural promoters ID IRON-BASED CATALYSTS; CARBON-DIOXIDE; CO; HYDROGENATION; POTASSIUM; ADSORPTION; HYDROCARBONS; FE(100); PHASE; MN AB Biomass gasification and subsequent conversion of this syngas to liquid hydrocarbons using Fischer-Tropsch (F-T) synthesis is a promising source of hydrocarbon fuels. However, biomass-derived syngas is different from syngas obtained from other sources such as steam reforming of methane. Specifically the H-2/CO ratio is less than 1/1 and the CO2 concentrations are somewhat higher. Here, we report the use of Fe-based F-T catalysts for the conversion of syngas produced by the air-blown, atmospheric pressure gasification of southern pine wood chips. The syngas from the gasification step is compressed and cleaned in a series of sorbents to produce the following feed to the F-T step: 2.78 % CH4, 11 % CO2, 15.4 % H-2, 21.3 % CO, and balance N-2. The relatively high level of CO2 suggests the need to use catalysts that are active for CO2 hydrogenation as well is resistant to oxidation in presence of high levels of CO2. The work reported here focuses on the effect of these different structural promoters on iron-based F-T catalysts with the general formulas 100Fe/5Cu/4K/15Si, 100Fe/5Cu/4K/15Al and 100Fe/5Cu/4K/15Zn. Although the effect of Si, Al or Zn on iron-based F-T catalysts has been examined previously for CO+CO2 hydrogenation, we have found no direct comparison of these three structural promoters, nor any studies of these promoters for a syngas produced from biomass. Results show that catalysts promoted with Zn and Al have a higher extent of reduction and carburization in CO and higher amount of carbides and CO adsorption as compared to Fe/Cu/K/Si. This resulted in higher activity and selectivity to C-5+ hydrocarbons than the catalyst promoted with silica. C1 [Sharma, Pratibha; Spivey, James J.] Louisiana State Univ, Dept Chem Engn, Baton Rouge, LA 70803 USA. [Elder, Thomas; Groom, Leslie H.] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. RP Spivey, JJ (reprint author), Louisiana State Univ, Dept Chem Engn, S Stadium Dr, Baton Rouge, LA 70803 USA. EM jjspivey@lsu.edu FU U.S. Department of Agriculture [11-DG-11221636-187] FX This material is based upon work funded by the U.S. Department of Agriculture, under Award Number 11-DG-11221636-187. We thank Dr. Kerry Dooley at Louisiana State University for providing the adsorbents for gas clean up, Dr. Gary Jacobs at University of Kentucky for liquid sample analysis, Ms. Wanda LeBlanc at Louisiana State University for helping in getting the XRD data and Mr. Tom Beasley at Florida International University for the SEM experiments. NR 56 TC 6 Z9 6 U1 7 U2 81 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1022-5528 EI 1572-9028 J9 TOP CATAL JI Top. Catal. PD APR PY 2014 VL 57 IS 6-9 BP 526 EP 537 DI 10.1007/s11244-013-0209-9 PG 12 WC Chemistry, Applied; Chemistry, Physical SC Chemistry GA AC9BO UT WOS:000332829200014 ER PT J AU Tian, JC Wang, XP Long, LP Romeis, J Naranjo, SE Hellmich, RL Shelton, AM AF Tian, Jun-Ce Wang, Xiang-Ping Long, Li-Ping Romeis, Joerg Naranjo, Steven E. Hellmich, Richard L. Shelton, Anthony M. TI Eliminating host-mediated effects demonstrates Bt maize producing Cry1F has no adverse effects on the parasitoid Cotesia marginiventris SO TRANSGENIC RESEARCH LA English DT Article DE Cry1F; Biosafety; Spodoptera frugiperda; Non-target effects; Study design ID TRANSGENIC INSECTICIDAL CROPS; BACILLUS-THURINGIENSIS; FALL ARMYWORM; LEPIDOPTERA-NOCTUIDAE; NATURAL ENEMIES; NONTARGET ARTHROPODS; RESISTANCE; HYMENOPTERA; BRACONIDAE; PLANTS AB The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is an important pest of maize in the United States and many tropical areas in the western hemisphere. In 2001, Herculex I-A (R) (Cry1F) maize was commercially planted in the United States to control Lepidoptera, including S. frugiperda. In 2006, a population of S. frugiperda was discovered in Puerto Rico that had evolved resistance to Cry1F maize in the field, making it the first well-documented case of an insect with field resistance to a plant producing protein from Bacillus thuringiensis (Bt). Using this resistant population, we conducted tri-trophic studies with a natural enemy of S. frugiperda. By using resistant S. frugiperda, we were able to overcome possible prey-mediated effects and avoid concerns about potential differences in laboratory- or field-derived Bt resistance. We used the Cry1F-resistant S. frugiperda to evaluate effects of Cry1F on Cotesia marginiventris (Cresson) (Hymenoptera: Braconidae), a larval endoparasitoid of S. frugiperda, over five generations. Our results clearly demonstrate that Cry1F maize does not affect development, parasitism, survivorship, sex ratio, longevity or fecundity of C. marginiventris when they parasitize Cry1F maize-fed S. frugiperda. Furthermore, the level of Cry1F protein in the leaves was strongly diluted when transferred from Bt maize to S. frugiperda and was not detected in larvae, cocoons or adults of C. marginiventris. Our results refute previous reports of C. marginiventris being harmed by Bt proteins and suggest that such results were caused by prey-mediated effects due to using Bt-susceptible lepidopteran hosts. C1 [Tian, Jun-Ce; Wang, Xiang-Ping; Long, Li-Ping; Shelton, Anthony M.] Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. [Tian, Jun-Ce] Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou, Zhejiang, Peoples R China. [Wang, Xiang-Ping] Yangtze Univ, Coll Agr, Jinzhou, Hubei, Peoples R China. [Long, Li-Ping] Guangxi Acad Agr Sci, Rice Res Inst, Nanning, Guangxi, Peoples R China. [Romeis, Joerg] Agroscope Reckenholz Tanikon Res Stn ART, Zurich, Switzerland. [Naranjo, Steven E.] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ USA. [Hellmich, Richard L.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Dept Entomol, Ames, IA USA. RP Shelton, AM (reprint author), Cornell Univ, New York State Agr Expt Stn, Dept Entomol, Geneva, NY 14456 USA. EM ams5@cornell.edu FU USDA National Institute of Food and Agriculture [2010-33522-21772] FX This project was supported by the Biotechnology Risk Assessment Program Competitive Grant No. 2010-33522-21772 from the USDA National Institute of Food and Agriculture. We thank H. Collins and M. Cheung for technical assistance and Mike Strand for supplying the initial colony of C. marginiventris. NR 39 TC 6 Z9 6 U1 2 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0962-8819 EI 1573-9368 J9 TRANSGENIC RES JI Transgenic Res. PD APR PY 2014 VL 23 IS 2 BP 257 EP 264 DI 10.1007/s11248-013-9748-x PG 8 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology GA AD1YE UT WOS:000333028800005 PM 24026808 ER PT J AU Alvarado-Esquivel, C Romero-Salas, D Garcia-Vazquez, Z Crivelli-Diaz, M Barrientos-Morales, M Lopez-de-Buen, L Dubey, JP AF Alvarado-Esquivel, Cosme Romero-Salas, Dora Garcia-Vazquez, Zeferino Crivelli-Diaz, Margarita Barrientos-Morales, Manuel Lopez-de-Buen, Lorena Dubey, Jitender P. TI Seroprevalence and correlates of Toxoplasma gondii infection in domestic pigs in Veracruz State, Mexico SO TROPICAL ANIMAL HEALTH AND PRODUCTION LA English DT Article DE Pigs; Toxoplasma gondii; Seroprevalence; Epidemiology; Mexico ID SEROEPIDEMIOLOGY; DURANGO AB Toxoplasma gondii infection in pigs has epidemiological concern for its contributing role in human infections. We determined seroprevalence of T. gondii infection in 402 domestic pigs raised in backyards in Veracruz State, Mexico using the modified agglutination test (MAT; cut off 1:25); 182 (45.3 %) of the 402 pigs were seropositive with MAT titers of 1:25 in 28, 1:50 in 22, 1:100 in 18, 1:200 in 30, 1:400 in 35, 1:800 in 23, 1:1,600 in 11, and 1:3,200 or higher in 15. Seropositive pigs were found in 137 (53.3 %) of 257 homes in all 7 municipalities surveyed. Multivariate analysis showed that T. gondii seropositivity in pigs was associated with tropical-humid climate (OR = 4.32; 95 % CI 1.47-12.62; P = 0.007) of the raising municipalities, feeding with leftovers (OR = 2.83; 95 % CI 1.01-7.91; P = 0.04), storing pig food in the owner's home (OR = 2.39; 95 % CI 1.09-5.22; P = 0.02), and free ranging (OR = 3.48; 95 % CI 1.49-8.15; P = 0.003). Results indicate that backyard pigs in Veracruz have the highest seroprevalence of T. gondii infection obtained by MAT in pigs studied in Mexico so far. The correlates of T. gondii infection found in the present study may be useful for an optimal planning of preventive measures against T. gondii infection in pigs. Results also remark the risk of T. gondii infection in humans by ingestion of raw or undercook pork in Mexico. C1 [Alvarado-Esquivel, Cosme] Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Durango 34000, Mexico. [Romero-Salas, Dora; Crivelli-Diaz, Margarita; Barrientos-Morales, Manuel; Lopez-de-Buen, Lorena] Univ Veracruzana, Fac Med Vet & Zootecnia, Lab Parasitol, Veracruz 91710, Mexico. [Garcia-Vazquez, Zeferino] Ctr Nacl Invest Disciplinaria Parasitol Vet, INIFAP, Jiutepec 6225, Morelos, Mexico. [Dubey, Jitender P.] ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. RP Dubey, JP (reprint author), ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, USDA, Bldg 1001, Beltsville, MD 20705 USA. EM jitender.dubey@ars.usda.gov NR 14 TC 5 Z9 5 U1 1 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-4747 EI 1573-7438 J9 TROP ANIM HEALTH PRO JI Trop. Anim. Health Prod. PD APR PY 2014 VL 46 IS 4 BP 705 EP 709 DI 10.1007/s11250-014-0551-3 PG 5 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AD1HK UT WOS:000332984700018 PM 24510223 ER PT J AU Maynard, JJ Dahlgren, RA O'Geen, AT AF Maynard, Jonathan J. Dahlgren, Randy A. O'Geen, Anthony T. TI Autochthonous and Allochthonous Carbon Cycling in a Eutrophic Flow-Through Wetland SO WETLANDS LA English DT Article DE Carbon cycling; Aquatic metabolism; Autochthonous carbon; Allochthonous carbon; Constructed wetlands; Primary productivity; Eutrophication; Water quality ID RECEIVING AGRICULTURAL RUNOFF; NONPOINT-SOURCE POLLUTION; DISSOLVED ORGANIC-CARBON; SAN-JOAQUIN VALLEY; AQUATIC FOOD WEBS; CONSTRUCTED WETLANDS; ECOSYSTEM METABOLISM; TERRESTRIAL SUPPORT; C-13 ADDITION; OXYGEN-DEMAND AB Wetland environments are important sites for the cycling and retention of terrestrially derived organic matter and nutrients. Wetland treatment of agricultural runoff has been shown to improve water quality and promote carbon sequestration. However, the potential role of eutrophic wetlands as a source of algal loading contributing to downstream hypoxia has prompted interest in understanding algal productivity and export from these systems. This study, in the San Joaquin Valley, California, quantified a mass balance of carbon and nutrients within a seasonally-saturated constructed wetland receiving agricultural runoff, as well as quantifying autochthonous carbon production on four sampling dates during a year with minimal emergent vegetation. Results from this study show that the wetland was a net-sink for nutrients and particulate/dissolved organic carbon. Despite high concentrations of inflowing nutrients and high rates of primary productivity, high respiration rates limited net organic C production and export due to high heterotrophic activity. The addition of high C loads in inflowing water and moderate retention efficiencies, however, resulted in a positive C retention during most sampling dates. This study provides valuable insight into the connection between elevated carbon and nutrient inflows, their effects on autochthonous carbon production, and resulting carbon and nutrient outflows. C1 [Maynard, Jonathan J.] US EPA, Corvallis, OR 97333 USA. [Dahlgren, Randy A.; O'Geen, Anthony T.] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95618 USA. RP Maynard, JJ (reprint author), NMSU, USDA ARS Jornada Expt Range, MSC 3 JER,Box 30003, Las Cruces, NM 88003 USA. EM jmaynard@nmsu.edu FU California State Water Resources Control Board; Kearney Foundation of Soil Science FX Funding for this project was provided through an agreement with the California State Water Resources Control Board and the Kearney Foundation of Soil Science. The information in this document has been subjected to review by the US Environmental Protection Agency and approved for publication. The contents of this document do not necessarily reflect the views and policies of the California State Water Resources Control Board or the US Environmental Protection Agency, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. We would like to thank Mickey Saso for his cooperation and support. We would also like to thank James Chang and Jiayou Deng for help with field sampling and laboratory analysis. NR 56 TC 1 Z9 1 U1 5 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0277-5212 EI 1943-6246 J9 WETLANDS JI Wetlands PD APR PY 2014 VL 34 IS 2 BP 285 EP 296 DI 10.1007/s13157-013-0474-1 PG 12 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AC9AE UT WOS:000332825400009 ER PT J AU Fleming, GM Wunderle, JM Ewert, DN O'Brien, JJ AF Fleming, Genie M. Wunderle, Joseph M. Ewert, David N. O'Brien, Joseph J. TI Estimating plant biomass in early-successional subtropical vegetation using a visual obstruction technique SO APPLIED VEGETATION SCIENCE LA English DT Article DE Subtropical shrubland; Robel pole; Bahamian coppice; Structurally diverse vegetation; Above-ground plant biomass ID ABOVEGROUND BIOMASS; MODEL SELECTION; WOODY-PLANTS; TRANSFORMATION; ELEUTHERA; HABITATS; BAHAMAS; BIAS AB Aim Non-destructive methods for quantifying above-ground plant biomass are important tools in many ecological studies and management endeavours, but estimation methods can be labour intensive and particularly difficult in structurally diverse vegetation types. We aimed to develop a low-cost, but reasonably accurate, estimation technique within early-successional Bahamian broadleaf shrub vegetation. Location Eleuthera, The Bahamas. Methods Our biomass estimation technique was based on a visual obstruction method originally developed for use in grassland vegetation, but modified to suit our mixed vegetation structure. Visual obstruction measures were recorded for a total of 90 0.25-m(2) quadrats from which all standing vegetation was subsequently collected, dried and weighed. Regression models were then developed to predict vegetation dry weight (biomass) from visual obstruction. Results The field time and equipment costs required to obtain our visual obstruction measures were low, and the accuracy of our above-ground plant biomass predictions was on par with that reported for other estimation techniques. Should increased accuracy or additional structural information be desired, our basic technique can be enhanced by adding other easily obtained measures (e.g. woody stem circumference). Conclusions Our low-cost technique yields reasonably accurate estimates of above-ground plant biomass and should be transferable to any shrubland vegetation type where the maximum height of vegetation is typically below 2m. C1 [Fleming, Genie M.] Puerto Rican Conservat Fdn, San Juan, PR 00936 USA. [Fleming, Genie M.; Wunderle, Joseph M.] USDA, Forest Serv, Int Inst Trop Forestry, Sabana Field Res Stn, Luquillo, PR 00773 USA. [Ewert, David N.] Nature Conservancy, Lansing, MI 48906 USA. [O'Brien, Joseph J.] USDA, Forest Serv, Ctr Forest Disturbance Sci, Athens, GA 30602 USA. RP Fleming, GM (reprint author), Puerto Rican Conservat Fdn, POB 362495, San Juan, PR 00936 USA. EM geniefleming@cox.net; jmwunderle@gmail.com; dewert@tnc.org; jjobrien@fs.fed.us FU U.S. Department of Agriculture Forest Service; Bahamas National Trust; College of the Bahamas; University of Puerto Rico; Kirtland's Warbler Recovery Team FX We are particularly grateful for the support we have received for our on-going research from the management and staff of Cape Eleuthera Resort. Jennifer Howard, Ronald Lance and Zeko McKenzie contributed to field data collection and processing of plant material. Funding was provided by International Programs of the U.S. Department of Agriculture Forest Service to The Nature Conservancy and the Puerto Rican Conservation Foundation working in cooperation with the Bahamas National Trust, the College of the Bahamas, the University of Puerto Rico and the Kirtland's Warbler Recovery Team. Helpful comments on early versions of the manuscript were provided by Thomas J. Brandeis, Patricia K. Lebow and Roger D. Ottmar. NR 25 TC 1 Z9 1 U1 3 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1402-2001 EI 1654-109X J9 APPL VEG SCI JI Appl. Veg. Sci. PD APR PY 2014 VL 17 IS 2 BP 356 EP 366 DI 10.1111/avsc.12067 PG 11 WC Plant Sciences; Ecology; Forestry SC Plant Sciences; Environmental Sciences & Ecology; Forestry GA AC8HT UT WOS:000332774800016 ER PT J AU Anathakrishnan, R Sinha, DK Murugan, M Zhu, KY Chen, MS Zhu, YC Smith, CM AF Anathakrishnan, Radhika Sinha, Deepak K. Murugan, Marimuthu Zhu, Kun Yan Chen, Ming-Shun Zhu, Yu Cheng Smith, C. Michael TI Comparative gut transcriptome analysis reveals differences between virulent and avirulent Russian wheat aphids, Diuraphis noxia SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Biotype; Diuraphis noxia; Phloem feeder; Plant resistance; Buchnera; Transcriptome analysis ID EXPRESSED SEQUENCE TAGS; ACYRTHOSIPHON-PISUM; INSECTICIDE RESISTANCE; MYZUS-PERSICAE; SOUTH-AFRICA; LARVAL GUT; PEA APHIDS; BIOTYPE 2; POPULATIONS; HEMIPTERA AB The Russian wheat aphid, Diuraphis noxia, is a destructive pest of cereal crops that exhibits virulence to D. noxia resistance genes in wheat. Therefore, it is important to identify D. noxia virulence factors. The insect gut, the primary site of defense to ingested toxins, is also a likely site of differential gene expression in virulent insects. Comparative analyses of gut transcriptomes from virulent and avirulent D. noxia can improve an understanding of aphid gut physiology and may reveal factors critical to compatible D. noxia-wheat interactions. A total of 4,600 clones were sequenced from gut cDNA libraries prepared from avirulent (biotype 1) and virulent (biotype 2) D. noxia feeding on biotype 1-resistant wheat. A majority of the sequences (66 % in biotype 1, 64 % in biotype 2) matched those from the NR database. BLASTx analysis of sequences with the highest E-values revealed that 59 % of the biotype 1 sequences matched those of the pea aphid, Acyrthosiphon pisum. However, only 17 % of the biotype 2 sequences were similar to those of A. pisum. RT-qPCR expression analyses confirmed that the biotype 2 gut transcriptome differs significantly from that of biotype 1. A transcript coding the tRNA-Leu gene was significantly up-regulated in the biotype 2 transcriptome, strongly suggesting that leucine metabolism is a critical factor in biotype 2 survival. Many more transcripts encoding protease inhibitors occurred in the avirulent biotype 1 gut than in the gut of virulent biotype 2. However, more protease transcripts occurred in the biotype 2 gut than in the biotype 1 gut, suggesting that the avirulent biotype produces protease inhibitors in response to plant proteases. The virulent biotype 2 produces trypsin-like and chymotrypsin-like serine protease counter-defenses to overcome biotype 1-resistant plants. C1 [Sinha, Deepak K.; Zhu, Kun Yan; Chen, Ming-Shun; Smith, C. Michael] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. [Murugan, Marimuthu] Tamil Nadu Agr Univ, Dept Plant Mol Biol & Biotechnol, Ctr Plant Mol Biol, Coimbatore 641003, Tamil Nadu, India. [Chen, Ming-Shun] Kansas State Univ, USDA ARS, Manhattan, KS 66506 USA. [Zhu, Yu Cheng] USDA ARS JWDSRC, Stoneville, MS 38776 USA. RP Smith, CM (reprint author), Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. EM radhyka@gmail.com; dksinha@k-state.edu; muruganmarimuthu1969@gmail.com; kzhu@ksu.edu; mchen@ksu.edu; yc.zhu@ars.usda.gov; cmsmith@ksu.edu FU Kansas Wheat Commission; Kansas Crop Improvement Association; Kansas Agricultural Experiment Station; National Science Foundation [DBI 0421427] FX We thank Dr. Marcelo Ortigao for critical reviews of drafts of the manuscript. This research was supported by grants to CMS from the Kansas Wheat Commission, the Kansas Crop Improvement Association, and the Kansas Agricultural Experiment Station. This is contribution No. 13-356-J of the Kansas Agricultural Experiment Station. This research was performed in the Gene Expression Facility at Kansas State University, which was supported through the National Science Foundation Grant, DBI 0421427. NR 54 TC 6 Z9 6 U1 9 U2 54 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD APR PY 2014 VL 8 IS 2 BP 79 EP 88 DI 10.1007/s11829-014-9293-4 PG 10 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA AC6SR UT WOS:000332655600001 ER PT J AU Prasifka, JR Hulke, BS Seiler, GJ AF Prasifka, Jarrad R. Hulke, Brent S. Seiler, Gerald J. TI Pericarp strength of sunflower and its value for plant defense against the sunflower moth, Homoeosoma electellum SO ARTHROPOD-PLANT INTERACTIONS LA English DT Article DE Host plant resistance; Pyralidae; Helianthus annuus; Inbred; Hybrid ID LEPIDOPTERA; PYRALIDAE; RESISTANCE; PHYTOMELANIN; HELIANTHUS; ACHENES; LINES; GERMPLASM; MECHANISM; INSECTS AB Sunflower pericarps provide a barrier against seed feeding by larvae of the sunflower moth, Homoeosoma electellum. Pericarp hardening is thought to be accelerated by a phytomelanin layer beneath the hypodermis, but among germplasm with phytomelanin, broad variation in sunflower pericarp strength exists. To facilitate the use of pericarp strength in sunflower breeding, resistance to mechanical puncture was assessed for diverse sunflower germplasm, and feeding tests were used to evaluate whether differences in pericarp strength prevent H. electellum larvae from penetrating achenes. Test on field-grown sunflowers indicates that public restorer lines have lower pericarp strengths compared with maintainer lines and commercial hybrids at 14 days after the start of anthesis. Interspecific crosses or plant introduction (PI) accessions believed to be resistant to H. electellum, including PI 170415, comprised a group with exceptionally high pericarp strength relative to other germplasm. In subsequent tests on greenhouse-grown sunflowers, overall results were similar, but using field-grown plants provided greater statistical power. In choice tests with achenes that differed in pericarp strength, 7- to 9-day-old sunflower moth larvae fed more often on seed protected by a weaker pericarp, at a more than 5-to-1 ratio, while 10-day-old larvae fed indiscriminately. Pericarp strength data contradict previous published results for individual entries and heterotic groups, but support the generalization that improved physical resistance to the sunflower moth is possible. To use pericarp strength in PI 170415 or similar sources, the inheritance of high pericarp strength and potential trade-offs between pericarp strength and other agronomic traits need to be understood. C1 [Prasifka, Jarrad R.; Hulke, Brent S.; Seiler, Gerald J.] ARS, No Crop Sci Lab, USDA, Fargo, ND 58102 USA. RP Prasifka, JR (reprint author), ARS, No Crop Sci Lab, USDA, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM jarrad.prasifka@ars.usda.gov NR 27 TC 3 Z9 5 U1 2 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1872-8855 EI 1872-8847 J9 ARTHROPOD-PLANT INTE JI Arthropod-Plant Interact. PD APR PY 2014 VL 8 IS 2 BP 101 EP 107 DI 10.1007/s11829-014-9290-7 PG 7 WC Ecology; Entomology SC Environmental Sciences & Ecology; Entomology GA AC6SR UT WOS:000332655600003 ER PT J AU Witwer, KW Hirschi, KD AF Witwer, Kenneth W. Hirschi, Kendal D. TI Transfer and functional consequences of dietary microRNAs in vertebrates: Concepts in search of corroboration SO BIOESSAYS LA English DT Article DE cholesterol; diet; epigenetics; exosome; microRNA; nutrition; RNAi ID DOUBLE-STRANDED-RNA; TRANSMEMBRANE PROTEIN SID-1; CROSS-KINGDOM REGULATION; I-LIKE RECEPTORS; CAENORHABDITIS-ELEGANS; C-ELEGANS; SYSTEMIC RNAI; HUMAN-CELLS; RIG-I; EXPRESSING BACTERIA AB If validated, diet-derived foreign microRNA absorption and function in consuming vertebrates would drastically alter our understanding of nutrition and ecology. RNA interference (RNAi) mechanisms of Caenorhabditis elegans are enhanced by uptake of environmental RNA and amplification and systemic distribution of RNAi effectors. Therapeutic exploitation of RNAi in treating human disease is difficult because these accessory processes are absent or diminished in most animals. A recent report challenged multiple paradigms, suggesting that ingested microRNAs (miRNAs) are transferred to blood, accumulate in tissues, and exert canonical regulation of endogenous transcripts. Independent replication of these findings has been elusive, and multiple disconfirmatory findings have been published. In the face of mounting negative results, any additional positive reports must provide the proverbial extraordinary proof to support such claims. In this article, we review the evidence for and against a significant role for dietary miRNAs in influencing gene expression, and make recommendations for future studies. Also watch the C1 [Witwer, Kenneth W.] Johns Hopkins Univ, Dept Mol & Comparat Pathobiol, Baltimore, MD 21218 USA. [Hirschi, Kendal D.] ARS, Dept Pediat, USDA, Childrens Nutr Res Ctr,Baylor Coll Med, Houston, TX USA. RP Witwer, KW (reprint author), Johns Hopkins Univ, Dept Mol & Comparat Pathobiol, Baltimore, MD 21218 USA. EM kwitwer1@jhmi.edu RI Witwer, Kenneth/G-1626-2013 OI Witwer, Kenneth/0000-0003-1664-4233 FU NIH [R21 AI102659, P30 MHO75673, R01 NS076357]; USDA/ARS [6250-51000-051]; Dry Bean Health Research Program; United Soybean Board FX The authors thank Adam Gillum for figure preparation and Jian Yang for reviewing the manuscript. K.W.W. is supported in part by NIH grants R21 AI102659 and P30 MHO75673, as well as R01 NS076357 (to J.E. Clements). K.D.H. acknowledges support from USDA/ARS under the Cooperative Agreement 6250-51000-051 and the Dry Bean Health Research Program and the United Soybean Board. NR 125 TC 40 Z9 40 U1 1 U2 31 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0265-9247 EI 1521-1878 J9 BIOESSAYS JI Bioessays PD APR PY 2014 VL 36 IS 4 BP 394 EP 406 DI 10.1002/bies.201300150 PG 13 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA AC2XE UT WOS:000332378800009 PM 24436255 ER PT J AU Lightle, D Lee, J AF Lightle, Danielle Lee, Jana TI Raspberry viruses affect the behavior and performance of Amphorophora agathonica in single and mixed infections SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE Aphididae; behavioral manipulation; Reoviridae; Raspberry latent virus; Closteroviridae; Rubus idaeus; co-infection; Rosaceae; Raspberry leaf mottle virus; Hemiptera; large raspberry aphid ID YELLOW DWARF VIRUS; MYZUS-PERSICAE HOMOPTERA; PLANT-VIRUSES; APHID VECTORS; POPULATION INCREASE; POTATO PLANTS; MOSAIC-VIRUS; HOST PLANTS; SUGAR-BEET; TRANSMISSION AB Pathogens may alter their hosts, which consequently increases transmission efficiency by vectors. We examined the effects of Raspberry leaf mottle virus [RLMV; Closterovirus (Closteroviridae)] and Raspberry latent virus [RpLV; Reovirus (Reoviridae)], alone and in a co-infection in raspberry, Rubus idaeus L. (Rosaceae) cv. Meeker, on the behavior and performance of its vector, Amphorophora agathonica Hottes (Hemiptera: Aphididae). Longevity was increased in aphids feeding on all infected-plant treatments compared with healthy plants, but aphid fecundity only increased in the co-infection treatment. In a two-way choice study between infected and healthy plants, aphids showed no difference in preference between plants after 30min of exposure. After 24h, aphids significantly preferred to settle on plants infected with RLMV over healthy, but healthy plants over plants infected with RpLV. There were no differences in settling preferences between healthy and co-infected plants. An electrical penetration graph study showed no differences in aphid feeding behavior on plants infected with RLMV and RLMV+RpLV when compared with healthy controls. Our results are consistent with past findings that infected plant's impact vector performance and behavior, but also highlight the need to further investigate greater virus diversity and effects of mixed infections. C1 [Lightle, Danielle] Oregon State Univ, Entomol Program, Corvallis, OR 97331 USA. [Lee, Jana] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97331 USA. RP Lightle, D (reprint author), POB 697,821 E South St, Orland, CA 95963 USA. EM lightled@onid.orst.edu FU USDA Specialty Crops Research Initiative grant [2009-51181-06022, CRIS 5358-22000-032-00D]; Western Sustainable Agricultural Research and Education Program [GW12-022] FX We thank C. Fieland and J. Mindolovich for assistance with behavioral assays, and N. Mosier and K. Sarver for help grafting the test plants. This article was greatly improved through comments provided by E. Backus and an anonymous reviewer. Raspberry plants were kindly donated by North American Plants. Funding was provided by USDA Specialty Crops Research Initiative grant 2009-51181-06022, CRIS 5358-22000-032-00D, and Western Sustainable Agricultural Research and Education Program GW12-022. NR 38 TC 0 Z9 0 U1 2 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD APR PY 2014 VL 151 IS 1 BP 57 EP 64 DI 10.1111/eea.12170 PG 8 WC Entomology SC Entomology GA AC5UL UT WOS:000332586400007 ER PT J AU Zhou, HP Fang, H Sanogo, S Hughs, SE Jones, DC Zhang, JF AF Zhou, Huiping Fang, Hui Sanogo, Soum Hughs, Sidney E. Jones, Don C. Zhang, Jinfa TI Evaluation of Verticillium wilt resistance in commercial cultivars and advanced breeding lines of cotton SO EUPHYTICA LA English DT Article DE Cotton; Germplasm; Verticillium wilt; Resistance ID DAHLIAE; YIELD; INHERITANCE; GERMPLASM; TOLERANCE AB Verticillium wilt (VW), caused by Verticillium dahliae Kleb, is one of the most destructive diseases in cotton (Gossypium spp.). The most efficient and cost-effective method of controlling the disease is the use of resistant cotton cultivars. Most commercial cultivars and elite breeding lines are developed under non-VW conditions and their responses to the disease are currently unknown. This study was conducted to evaluate current commercial cotton cultivars and advanced breeding lines for VW resistance. In 2011-2013, a total of 84 cultivars from major US seed companies, 52 advanced breeding lines from the US public breeding programs, and 87 introgression lines from a cross between Acala 1517-99 x Pima PHY 76 from the New Mexico Cotton Breeding Program, were evaluated for VW resistance in the greenhouse. Cotton cultivars and breeding lines were evaluated in ten separate replicated tests by inoculation with a defoliating-type isolate of V. dahliae. While leaf severity rating and percentages of infected plants, infected leaves and defoliated leaves were found to be significantly and positively correlated with one another, leaf severity rating and percentage of infected leaves were best choices because of their relatively low coefficients of variation and higher resolutions to differentiate resistant genotypes from susceptible ones. The heritabilities for the VW resistance traits ranged from 0.58 to 0.80 with an average of 0.67, indicating that variation in VW resistance is predominantly due to genetic factors. Of the 223 commercial cultivars and advanced lines, six Upland cultivars (FM 9160B2F, FM 9170 B2F, NG 4010 B2RF, Nitro 44 B2RF, DP 1219 B2RF, and ST 4288 B2F), five advanced lines (Ark 0403-3, MD 10-5, MD 25ne, NC11AZ01, and PD 0504), two introgression lines from Upland x Pima (NM11Q1157 and 08N1618), and four Pima cultivars (COBALT, DP 357, PHY 800, and PHY 830) had higher levels of resistance to VW. The resistance shown by most of these cultivars in the greenhouse was consistent with their performance in previous field tests. Based on the initial VW resistance, 19 highly or moderately resistant genotypes were chosen for re-evaluation and 30 genotypes were also assessed more than once for VW resistance in different tests, most of which had concordant performance. These cultivars and advanced lines should be useful resources to improve VW resistance in cotton breeding. C1 [Zhou, Huiping; Fang, Hui; Zhang, Jinfa] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Sanogo, Soum] New Mexico State Univ, Dept Entomol Plant Pathol & Weed Sci, Las Cruces, NM 88003 USA. [Hughs, Sidney E.] USDA ARS, Southwestern Cotton Ginning Res Lab, Mesilla Pk, NM 88047 USA. [Jones, Don C.] Cotton Inc, Cary, NC USA. RP Zhang, JF (reprint author), New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. EM jinzhang@nmsu.edu RI Fang, Hui/R-9813-2016; Zhou, Huiping/R-9888-2016 OI Fang, Hui/0000-0002-2693-5379; Zhou, Huiping/0000-0002-2346-6909 FU USDA-ARS; Cotton Incorporated; New Mexico Agricultural Experiment Station FX The study was in part supported by USDA-ARS, Cotton Incorporated and New Mexico Agricultural Experiment Station. The authors thank the US public cotton geneticists and breeders, and seed companies for providing the seeds used in this study. NR 28 TC 9 Z9 11 U1 1 U2 20 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD APR PY 2014 VL 196 IS 3 BP 437 EP 448 DI 10.1007/s10681-013-1045-5 PG 12 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AC6AV UT WOS:000332603800011 ER PT J AU Hayes, CM Rooney, WL AF Hayes, Chad M. Rooney, William L. TI Agronomic performance and heterosis of specialty grain sorghum hybrids with a black pericarp SO EUPHYTICA LA English DT Article DE Grain sorghum; Tannins; Phenols; 3-Deoxyanthocyanin; Genotype x environment interaction ID 3-DEOXYANTHOCYANINS; PHYTOCHEMICALS; INHIBITION; GENOTYPES AB Specialty food markets are continually interested in food grains with unique attributes and qualities. The objective of this study was to evaluate the relative effects of genotype and environment on the agronomic performance of six black grain sorghum hybrids developed to produce high concentrations of total phenols, condensed tannins and 3-deoxyanthocyanin. These hybrids and a commercial grain sorghum check were evaluated in six Texas environments (three locations and two years). Significant effects due to genotype and environment were detected. Interaction between genotype and environment were also detected but they were smaller in magnitude than the main effects. Compared to the commercial check, the black sorghum hybrids had significantly higher concentrations of phenols, tannins and 3-deoxyanthocyanins across all environments but the grain yield of the best hybrid was only 78 % that of the commercial check. Heterosis for grain yield was detected but phenol, tannin and 3-deoxyanthocyanin concentration did not express high parent heterosis. The results indicate that higher yield is possible in black sorghum hybrids and selection should be effective in improving concentrations of phenols, tannins and 3-deoxyanthocyanin. C1 [Hayes, Chad M.] USDA ARS, Cropping Syst Res Lab, Lubbock, TX 79415 USA. [Rooney, William L.] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. RP Rooney, WL (reprint author), Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA. EM wlr@tamu.edu NR 19 TC 6 Z9 7 U1 4 U2 16 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD APR PY 2014 VL 196 IS 3 BP 459 EP 466 DI 10.1007/s10681-013-1047-3 PG 8 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AC6AV UT WOS:000332603800013 ER PT J AU Hruska, Z Yao, HB Kincaid, R Brown, R Cleveland, T Bhatnagar, D AF Hruska, Zuzana Yao, Haibo Kincaid, Russell Brown, Robert Cleveland, Thomas Bhatnagar, Deepak TI Fluorescence Excitation-Emission Features of Aflatoxin and Related Secondary Metabolites and Their Application for Rapid Detection of Mycotoxins SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Excitation-emission matrix; Fluorescence hyperspectral imaging; Aflatoxin; Secondary metabolites; Food and feed safety ID GREENISH-YELLOW-FLUORESCENCE; PERFORMANCE LIQUID-CHROMATOGRAPHY; ASPERGILLUS-FLAVUS; OCHRATOXIN-A; COTTON LINT; KOJIC ACID; CORN; CONTAMINATION; MATRIX; CLEANUP AB The persistent occurrence of aflatoxins in food and feed remains a problem for producers of commodities subject to colonization with toxigenic molds. Aflatoxins are secondary metabolites of fungi of the Aspergillus spp. associated with deleterious health effects. Because current screening methods for these toxins are lengthy, destructive, and costly, there is a continuous search for a more rapid, noninvasive, and cost-effective technology. The present study utilized a fluorescence excitation-emission matrix (EEM) of aflatoxin as well as two additional secondary metabolites (kojic acid and the bright greenish-yellow fluorescence (BGYF) compound) of Aspergillus flavus measured with a fluorescence spectrophotometer. The results were compared to image data acquired with a fluorescence hyperspectral sensor in order to evaluate the potential of image-based technology for detecting aflatoxin in grain. The excitation-emission matrix of aflatoxin B1 standard produced overlapping peaks in 340-400 nm of excitation range emitting in the blue range at around 450 nm. The spectral signature extracted from the hyperspectral image was also in the blue range, emitting blue fluorescence. Because the results from both systems were comparable, where all fluorescence peaks were in the blue range, the present study validates the feasibility of image-based technology for nondestructive detection of aflatoxin in corn. Additional peaks were revealed in the aflatoxin EEM in the 260-nm excitation range that were not present in the kojic acid and BGYF compound mixture. This new information allows for the separation of the aflatoxin signature from the potentially confounding overlap of other secondary metabolites occurring in the blue and blue-green spectral ranges. C1 [Hruska, Zuzana; Yao, Haibo; Kincaid, Russell] Mississippi State Univ, Geosyst Res Inst, MSU Sci & Technol Ctr, Stennis Space Ctr, Stennis Space Ctr, MS 39529 USA. [Brown, Robert; Cleveland, Thomas; Bhatnagar, Deepak] Agr Res Serv, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Hruska, Z (reprint author), Mississippi State Univ, Geosyst Res Inst, MSU Sci & Technol Ctr, Stennis Space Ctr, 1021 Balch Blvd, Stennis Space Ctr, MS 39529 USA. EM hruska@gri.msstate.edu FU USDA [58-6435-3-121]; MSU [58-6435-3-121] FX Funding for this study was provided by the cooperative agreement no. 58-6435-3-121 between USDA and MSU. The authors gratefully acknowledge the assistance provided by Professor Laodong Guo and his graduate student Zhengzhen Zhou from the University of Southern Mississippi for EEM data acquisition and instrumentation expertise, and Mr. Lee Hathcock (Mississippi State University) for radiometric calibration of the hyperspectral data. NR 32 TC 6 Z9 6 U1 3 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD APR PY 2014 VL 7 IS 4 BP 1195 EP 1201 DI 10.1007/s11947-014-1265-2 PG 7 WC Food Science & Technology SC Food Science & Technology GA AC5CN UT WOS:000332538600024 ER PT J AU Volpe, R AF Volpe, Richard TI Supercenters, Unionized Labor, and Performance in Food Retail SO INDUSTRIAL RELATIONS LA English DT Article ID WAL-MART; INFORMATION-TECHNOLOGY; SUPERMARKET INDUSTRY; MONOPOLY PROFITS; COMPETITION; ENTRY; PRICES; IMPACT; SALES; CHAIN AB This study examines the impact of unionized labor on supermarket performance, as measured by profit and sales, accounting for the competitive presence of supercenters. The results confirm prior research that shows that supercenters have negative effects on supermarket performance. Unionized supermarkets generally outperform nonunionized supermarkets. However this effect disappears when accounting for supercenters, largely because unionized stores are less likely to compete with supercenters. I find no evidence for a significant union effect on supermarket performance. The deleterious effects of supercenters are stronger for unionized stores. Unionized supermarkets utilize less full-time labor and more labor-saving technology than do nonunionized ones. C1 USDA ERS, Washington, DC 20250 USA. RP Volpe, R (reprint author), USDA ERS, Washington, DC 20250 USA. EM rvolpe@ers.usda.gov NR 69 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0019-8676 EI 1468-232X J9 IND RELAT JI Ind. Relat. PD APR PY 2014 VL 53 IS 2 BP 325 EP 355 DI 10.1111/irel.12058 PG 31 WC Industrial Relations & Labor SC Business & Economics GA AC7KM UT WOS:000332707200006 ER PT J AU Vu, HS Roth, MR Tamura, P Samarakoon, T Shiva, S Honey, S Lowe, K Schmelz, EA Williams, TD Welti, R AF Vu, Hieu Sy Roth, Mary R. Tamura, Pamela Samarakoon, Thilani Shiva, Sunitha Honey, Samuel Lowe, Kaleb Schmelz, Eric A. Williams, Todd D. Welti, Ruth TI Head-group acylation of monogalactosyldiacylglycerol is a common stress response, and the acyl-galactose acyl composition varies with the plant species and applied stress SO PHYSIOLOGIA PLANTARUM LA English DT Article ID ARABIDOPSIS-THALIANA; ACYLGALACTOSYL DIGLYCERIDE; ACID; GALACTOLIPIDS; EXTRACTION; LEAVES AB Formation of galactose-acylated monogalactosyldiacylglycerols has been shown to be induced by leaf homogenization, mechanical wounding, avirulent bacterial infection and thawing after snap-freezing. Here, lipidomic analysis using mass spectrometry showed that galactose-acylated monogalactosyldiacylglycerols, formed in wheat (Triticum aestivum) and tomato (Solanum lycopersicum) leaves upon wounding, have acyl-galactose profiles that differ from those of wounded Arabidopsis thaliana, indicating that different plant species accumulate different acyl-galactose components in response to the same stress. Additionally, the composition of the acyl-galactose component of Arabidopsis acMGDG (galactose-acylated monogalactosyldiacylglycerol) depends on the stress treatment. After sub-lethal freezing treatment, acMGDG contained mainly non-oxidized fatty acids esterified to galactose, whereas mostly oxidized fatty acids accumulated on galactose after wounding or bacterial infection. Compositional data are consistent with acMGDG being formed in vivo by transacylation with fatty acids from digalactosyldiacylglycerols. Oxophytodienoic acid, an oxidized fatty acid, was more concentrated on the galactosyl ring of acylated monogalactosyldiacylglycerols than in galactolipids in general. Also, oxidized fatty acid-containing acylated monogalactosyldiacylglycerols increased cumulatively when wounded Arabidopsis leaves were wounded again. These findings suggest that, in Arabidopsis, the pool of galactose-acylated monogalactosyldiacylglycerols may serve to sequester oxidized fatty acids during stress responses. C1 [Vu, Hieu Sy; Roth, Mary R.; Tamura, Pamela; Samarakoon, Thilani; Shiva, Sunitha; Honey, Samuel; Lowe, Kaleb; Welti, Ruth] Kansas State Univ, Div Biol, Kansas Lipid Res Ctr, Manhattan, KS 66506 USA. [Samarakoon, Thilani] Kansas State Univ, Dept Chem, Manhattan, KS 66506 USA. [Schmelz, Eric A.] USDA ARS, Chem Res Unit, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Williams, Todd D.] Univ Kansas, Mass Spectrometry Lab, Lawrence, KS 66045 USA. RP Welti, R (reprint author), Kansas State Univ, Div Biol, Kansas Lipid Res Ctr, Ackert Hall, Manhattan, KS 66506 USA. EM welti@ksu.edu OI Welti, Ruth/0000-0003-4373-2538; Schmelz, Eric/0000-0002-2837-734X FU National Science Foundation [MCB 0920663] FX The authors would like to thank Dr Ernst Heinz for his encouragement and helpful comments. We are grateful to Drs Xuemin Wang and Jyoti Shah for their participation in related studies. We thank Dr Mark Ungerer for use of a freezing chamber and Dr Ari Jumponen for use of a light cart. We appreciate Dr Sunish Sehgal allowing us to harvest wheat leaves from his plantings. This work was supported by the National Science Foundation (MCB 0920663 to R. W.). Instrument acquisition at the Kansas Lipidomics Research Center was supported by National Science Foundation (EPS 0236913, DBI 0521587, DBI 1228622), Kansas Technology Enterprise Corporation, K-IDeA Networks of Biomedical Research Excellence (INBRE) of National Institutes of Health (P20RR16475) and Kansas State University. Contribution no. 13-362-J from the Kansas Agricultural Experiment Station. NR 18 TC 7 Z9 7 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD APR PY 2014 VL 150 IS 4 BP 517 EP 528 DI 10.1111/ppl.12132 PG 12 WC Plant Sciences SC Plant Sciences GA AC5IE UT WOS:000332553600003 PM 24286212 ER PT J AU Bruckart, WL Eskandari, FM Berner, DK AF Bruckart, W. L., III Eskandari, F. M. Berner, D. K. TI Characterization and evaluation of Ramularia crupinae, a candidate for biological control, and of its host, two varieties of Crupina vulgaris in the United States SO BIOLOGICAL CONTROL LA English DT Article DE Asteraceae; Cardueae; Fungi; Host range; Invasive weed; Risk assessment ID JACEAE VAR. SOLSTITIALIS; SUPPLEMENTAL RISK EVALUATIONS; WESTERN NORTH-AMERICA; YELLOW STARTHISTLE; COMMON CRUPINA; PUCCINIA-CARDUORUM; RUSSIAN THISTLE; CONTROL AGENT; MUSK THISTLE; RANGE AB Crupina vulgaris (common crupina) is an invasive annual plant of rangelands and pastures in the United States (USA). There are two varieties of C. vulgaris in the USA that differ morphologically and biologically, particularly in requirements for bolting. A vernalization protocol enabled synchronization of bolting, thus facilitating concurrent comparative studies of both varieties. Leaf-removal experiments suggest that all leaves provide photosynthate for seed fill, but the cauline leaves, on a relative basis, produced 1.7-4 times more seed per g than rosette leaves, suggesting disease on the cauline leaves has greater potential to reduce seed production than disease on rosette leaves. Ramularia crupinae, a leaf-spotting fungus, was evaluated for biological control. A single inoculation caused a 47% reduction in root dry weight, and seed yield was reduced by 39% as the number of inoculations increased from 0 (controls) to 3. None of 35 non-target taxa, i.e., species, cultivars, and varieties, in the tribe Cardueae was damaged from inoculation by R. crupinae, including safflower, which developed a few, small necrotic spots on old leaves. Best Linear Unbiased Predictors (BLUPs), generated by a mixed model analysis of disease reaction and genetic relatedness data, indicated that only C. vulgaris varieties were susceptible to disease; i. e., BLUPs were significantly different from zero (p > vertical bar t vertical bar < 0.05). None of the other taxa had BLUP estimators that were significantly different from zero and were, therefore, determined to be not susceptible to disease by R. crupinae. Results suggest that R. crupinae is a potentially good candidate for biological control of this important weed pest and would likely not affect other species of importance in the USA. Published by Elsevier Inc. C1 [Bruckart, W. L., III; Eskandari, F. M.; Berner, D. K.] ARS, USDA, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. RP Bruckart, WL (reprint author), ARS, USDA, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM william.bruckart@ars.usda.gov NR 39 TC 2 Z9 2 U1 13 U2 23 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2014 VL 71 BP 40 EP 48 DI 10.1016/j.biocontrol.2014.01.003 PG 9 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AC3HJ UT WOS:000332407500006 ER PT J AU Tipping, PW Martin, MR Pokorny, EN Nimmo, KR Fitzgerald, DL Dray, FA Center, TD AF Tipping, Philip W. Martin, Melissa R. Pokorny, Eileen N. Nimmo, Kayla R. Fitzgerald, Danyelle L. Dray, F. Allen, Jr. Center, Ted D. TI Current levels of suppression of waterhyacinth in Florida USA by classical biological control agents SO BIOLOGICAL CONTROL LA English DT Article DE Waterhyacinth; Biological control; Herbicides; Integration; Eichhornia crassipes ID HERBICIDE APPLICATION; WATERHYACINTH; HERBIVORY; INSECTS; PLANTS AB Waterhyacinth, Eichhornia crassipes (Mart.) Solms (Pontederiaceae), has been a global target for classical biological control efforts for decades. In Florida, herbicidal application is the primary control method employed, usually without regard for the activities of the three biological control agents introduced intentionally during the 1970s, namely Neochetina eichhorniae Warner, Neochetina bruchi, Hustache (Coleoptera: Curculionidae), and Niphograpta albiguttalis Warren (Lepidoptera: Crambidae). A series of field experiments from 2008 to 2010 was conducted at four Florida sites using an insecticide-check approach to quantify the current levels of suppression provided by these agents. In the field N. albiguttalis was rarely found while more than 99% of all Neochetina sp. adults were N. eichhorniae. Although it was not possible to disentangle the relative impacts of Neochetina sp. adults from larvae on individual plant variables, the larvae played a major role in reducing plant biomass and the number of inflorescences. Plots exposed to unrestricted herbivory contained 58.2% less biomass and produced 97.3% fewer inflorescences at the end of the experiments. Despite these large reductions, herbivory decreased waterhyacinth coverage by only 16.8% and most of this was attributed to a low-nutrient site where coverage was reduced disproportionately. Overall, coverage trended upwards during the course of the experiments and was always close to 100% when the plots were harvested. Although coverage is a somewhat arbitrary metric, especially for floating plants subject to compression and dispersion, it influences the perception of biological control efficacy which, in turn, directly influences herbicide management decisions in Florida. Despite waterhyacinth populations that now produce less than half as much biomass and up to 98% fewer seeds than before the deployment of biological control agents, the overall approach used to achieve maintenance control of the plant in Florida will probably not change unless new biological control agents, such as Megamelus scutellaris Berg (Hemiptera: Delphacidae), can reduce coverage significantly. Published by Elsevier Inc. C1 [Tipping, Philip W.; Pokorny, Eileen N.; Dray, F. Allen, Jr.; Center, Ted D.] USDA ARS, Invas Plant Management Lab, Ft Lauderdale, FL 33314 USA. [Martin, Melissa R.] US Fish & Wildlife, ARM Loxahatchee Wildlife Refuge, Delray Beach, FL USA. [Nimmo, Kayla R.] Dry Tortugas Natl Pk, Key West, FL USA. [Fitzgerald, Danyelle L.] Nova Univ, Ft Lauderdale, FL 33314 USA. RP Tipping, PW (reprint author), USDA ARS, Invas Plant Res Lab, 3225 Coll Ave, Ft Lauderdale, FL 33314 USA. EM philip.tipping@ars.usda.gov NR 20 TC 1 Z9 2 U1 3 U2 20 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2014 VL 71 BP 65 EP 69 DI 10.1016/j.biocontrol.2014.01.008 PG 5 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AC3HJ UT WOS:000332407500009 ER PT J AU Kelly, JL Hagler, JR Kaplan, I AF Kelly, Jessica L. Hagler, James R. Kaplan, Ian TI Semiochemical lures reduce emigration and enhance pest control services in open-field predator augmentation SO BIOLOGICAL CONTROL LA English DT Article DE Augmentation; HIPVs; Aggregation pheromone; Protein marking; Gut content analysis; Podisus maculiventris ID PALLAS COLEOPTERA-COCCINELLIDAE; PODISUS-MACULIVENTRIS HEMIPTERA; BIOLOGICAL-CONTROL AGENTS; POTATO BEETLE COLEOPTERA; INDUCED PLANT VOLATILES; STINKBUGS HEMIPTERA; HARMONIA-AXYRIDIS; METHYL SALICYLATE; BENEFICIAL INSECTS; BEMISIA-TABACI AB Augmentation biocontrol is a commercially viable pest management tactic in enclosed glasshouse environments, but is far less effective in open-field agriculture where newly released enemies rapidly disperse from release sites. We tested the potential for behavior-modifying semiochemicals to increase the retention of mass released predatory stink bugs, Podisus maculiventris Say (Hemiptera: Pentatomidae), for enhanced consumption of hornworm caterpillars, Manduca sexta L. (Lepidoptera: Sphingidae). To do so, we used controlled-release dispensers to emit the herbivore-induced plant volatile, methyl salicylate (MeSA), or P. maculiventris aggregation pheromone from tomato field plots. Overall, we recaptured ca. 17% of released individuals after 36 h. This rate, however, was significantly affected by weather (12% vs. 22% recapture in rainy vs. dry weeks, respectively) and semiochemical deployment, but only under optimal weather conditions (19% vs. 26% recapture in control vs. pheromone plots, respectively, during dry weeks). Further, we detected behavioral responses of wild P. maculiventris to semiochemical treatment with 94% of all captured adults (=84 of 89 total) found in pheromone plots. Only 24 of 567 (4%) captured stink bugs tested positive for immunomarking, suggesting that hornworm predation occurred but at a low frequency. Importantly, we documented that sentinel caterpillar prey were depleted by predators at a higher rate in stink bug augmented plots on tomato plants occurring near (<3 m from) the MeSA and pheromone lures. These data empirically demonstrate that both semiochemicals are capable of increasing pest consumption via attraction of P. maculiventris. Future work should focus on mechanisms of lure attraction and the long-term consequences of predator development in fields with elevated semiochemical emissions. (C) 2014 Elsevier Inc. All rights reserved. C1 [Kelly, Jessica L.; Kaplan, Ian] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Hagler, James R.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Kaplan, I (reprint author), Purdue Univ, Dept Entomol, 901 W State St, W Lafayette, IN 47907 USA. EM James.Hagler@ars.usda.gov; ikaplan@purdue.edu FU USDA-NIFA [2011-67013-30126] FX We thank USDA-NIFA, Grant 2011-67013-30126 for funding this work. We also thank Red Gold Inc. for donating tomato starts and Felisa Blackmer and Scott Machtley for technical support. Finally, we thank Douglas Richmond, Gina Angelella, Carmen Blubaugh, Joseph Braasch, Michael Garvey, Elizabeth Rowen and Ulianova Vidal Gomez for reviewing the experimental design and manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. NR 50 TC 8 Z9 8 U1 5 U2 70 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD APR PY 2014 VL 71 BP 70 EP 77 DI 10.1016/j.biocontrol.2014.01.010 PG 8 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AC3HJ UT WOS:000332407500010 ER PT J AU Oishi, AC Palmroth, S Johnsen, KH Mccarthy, HR Oren, R AF Oishi, A. Christopher Palmroth, Sari Johnsen, Kurt H. Mccarthy, Heather R. Oren, Ram TI Sustained effects of atmospheric [CO2] and nitrogen availability on forest soil CO2 efflux SO GLOBAL CHANGE BIOLOGY LA English DT Article DE automated soil respiration measurements; FACE; nitrogen fertilization; Pinus taeda; primary productivity; soil water content ID FINE-ROOT PRODUCTION; SOUTHERN UNITED-STATES; LOBLOLLY-PINE STANDS; CANOPY LEAF-AREA; LONG-TERM GROWTH; ELEVATED CO2; TEMPERATE FOREST; N-FERTILIZATION; CARBON ALLOCATION; TEMPORAL RESOLUTION AB Soil CO2 efflux (F-soil) is the largest source of carbon from forests and reflects primary productivity as well as how carbon is allocated within forest ecosystems. Through early stages of stand development, both elevated [CO2] and availability of soil nitrogen (N; sum of mineralization, deposition, and fixation) have been shown to increase gross primary productivity, but the long-term effects of these factors on F-soil are less clear. Expanding on previous studies at the Duke Free-Air CO2 Enrichment (FACE) site, we quantified the effects of elevated [CO2] and N fertilization on F-soil using daily measurements from automated chambers over 10years. Consistent with previous results, compared to ambient unfertilized plots, annual F-soil increased under elevated [CO2] (ca. 17%) and decreased with N (ca. 21%). N fertilization under elevated [CO2] reduced F-soil to values similar to untreated plots. Over the study period, base respiration rates increased with leaf productivity, but declined after productivity saturated. Despite treatment-induced differences in aboveground biomass, soil temperature and water content were similar among treatments. Interannually, low soil water content decreased annual F-soil from potential values - estimated based on temperature alone assuming nonlimiting soil water content - by ca. 0.7% per 1.0% reduction in relative extractable water. This effect was only slightly ameliorated by elevated [CO2]. Variability in soil N availability among plots accounted for the spatial variability in F-soil, showing a decrease of ca. 114gCm(-2)yr(-1) per 1gm(-2) increase in soil N availability, with consistently higher F-soil in elevated [CO2] plots ca. 127gC per 100ppm [CO2] over the +200ppm enrichment. Altogether, reflecting increased belowground carbon partitioning in response to greater plant nutritional needs, the effects of elevated [CO2] and N fertilization on F-soil in this stand are sustained beyond the early stages of stand development and through stabilization of annual foliage production. C1 [Oishi, A. Christopher; Palmroth, Sari; Oren, Ram] Duke Univ, Nicholas Sch Environm, Div Environm Sci & Policy, Durham, NC 27708 USA. [Oishi, A. Christopher] US Forest Serv, Coweeta Hyrdol Lab, Southern Res Stn, USDA, Otto, NC 28763 USA. [Palmroth, Sari; Oren, Ram] Swedish Univ Agr Sci SLU, Dept Forest Ecol & Management, SE-90183 Umea, Sweden. [Johnsen, Kurt H.] US Forest Serv, Southern Res Stn, USDA, Res Triangle Pk, NC 27709 USA. [Mccarthy, Heather R.] Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA. [Oren, Ram] Duke Univ, Pratt Sch Engn, Dept Civil & Environm Engn, Durham, NC 27708 USA. RP Oishi, AC (reprint author), Duke Univ, Nicholas Sch Environm, Div Environm Sci & Policy, Durham, NC 27708 USA. EM acoishi@fs.fed.us FU Office of Science (BER) of US Department of Energy through Terrestrial Carbon Processes (TCP) program (FACE) [DE-FG02-95ER62083, DE-SC0006967]; National Institute of Global Environmental Change (NIGEC) through the Southeast Regional Center at the University of Alabama, Tuscaloosa (DOE) [DE-FC030-90ER61010]; SERC-NIGEC RCIAP Research Program FX This research was supported by the Office of Science (BER) of US Department of Energy through Terrestrial Carbon Processes (TCP) program (FACE, DE-FG02-95ER62083 and DE-SC0006967), by the National Institute of Global Environmental Change (NIGEC) through the Southeast Regional Center at the University of Alabama, Tuscaloosa (DOE cooperative agreement DE-FC030-90ER61010), and by the SERC-NIGEC RCIAP Research Program. We thank John Butnor, Analie Barnett, Song Qian, and Eric Ward for assistance with data analysis, Robert Nettles for field support, and Daniel McInnis for technical assistance with the ACES and field support. We also thank the anonymous reviewers for their constructive comments. NR 61 TC 9 Z9 10 U1 5 U2 84 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD APR PY 2014 VL 20 IS 4 BP 1146 EP 1160 DI 10.1111/gcb.12414 PG 15 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AB8WA UT WOS:000332069500012 PM 24115580 ER PT J AU Nansen, C Meikle, WG AF Nansen, Christian Meikle, William G. TI Journal impact factors and the influence of age and number of citations SO MOLECULAR PLANT PATHOLOGY LA English DT Article C1 [Nansen, Christian] Univ Western Australia, Sch Anim Biol, Crawley, WA 6009, Australia. [Nansen, Christian] Univ Western Australia, UWA Inst Agr, Crawley, WA 6009, Australia. [Meikle, William G.] USDA ARS, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. RP Nansen, C (reprint author), Univ Western Australia, Sch Anim Biol, 35 Stirling Highway, Crawley, WA 6009, Australia. EM christian.nansen@uwa.edu.au NR 9 TC 2 Z9 2 U1 2 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD APR PY 2014 VL 15 IS 3 BP 223 EP 225 DI 10.1111/mpp.12096 PG 3 WC Plant Sciences SC Plant Sciences GA AC0WX UT WOS:000332217300001 PM 24597724 ER PT J AU Salis, M Ager, AA Finney, MA Arca, B Spano, D AF Salis, Michele Ager, Alan A. Finney, Mark A. Arca, Bachisio Spano, Donatella TI Analyzing spatiotemporal changes in wildfire regime and exposure across a Mediterranean fire-prone area SO NATURAL HAZARDS LA English DT Article DE Fire exposure; Fire regime; Mediterranean area; MTT algorithm ID CLIMATE-CHANGE; UNITED-STATES; FOREST-FIRES; NE SPAIN; RISK; BASIN; LANDSCAPE; PORTUGAL; SUPPRESSION; BEHAVIOR AB We evaluated the spatiotemporal changes in wildfire regime and exposure in a fire-prone Mediterranean area (Sardinia, Italy) in relation to changes in ignition patterns, weather, suppression activities, and land uses. We also used wildfire simulations to identify fine-scale changes in wildfire exposure of important features on the island. Sardinia experienced a sharp reduction in fire number and area burned between the periods 1980-1994 and 1995-2009. Despite this decrease, losses and fatalities from wildfires continue. This suggests that localized areas and seasons of high wildfire risk persist on the island. Our analysis showed (1) a reduction in area burned (60,000-20,000 ha/year) and ignitions (3,700-2,600 fires/year), (2) an advance of 15 days for the fire season peak, (3) an increase in spring temperatures, and (4) an increase in fire exposure for WUI areas. Little change was noted for land use types and associated fuels. Most likely the reduction in fire activity may be due to a combination of social factors and suppression capabilities. On the other hand, simulation modeling suggested pockets of high wildfire exposure in specific places. The combined empirical analyses and simulation modeling provided a robust approach to understanding the spatiotemporal dynamics of wildfire risk on the island. C1 [Salis, Michele; Spano, Donatella] Univ Sassari, Dept Sci Nat & Environm Resources DIPNET, I-07100 Sassari, Italy. [Salis, Michele; Spano, Donatella] Euro Mediterranean Ctr Climate Changes CMCC, IAFENT Div, I-07100 Sassari, Italy. [Ager, Alan A.] US Forest Serv, USDA, Pacific NW Res Stn, Western Wildland Environm Threat Assessment Ctr, Prineville, OR 97754 USA. [Finney, Mark A.] Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. [Arca, Bachisio] Natl Res Council CNR, Inst Biometeorol IBIMET, I-07100 Sassari, Italy. RP Salis, M (reprint author), Univ Sassari, Dept Sci Nat & Environm Resources DIPNET, Via De Nicola 9, I-07100 Sassari, Italy. EM miksalis@uniss.it FU GEMINA Project-MIUR/MATTM [232/2011]; FUME [243888]; Project "Modeling approach to evaluate fire risk and mitigation planning actions'' FX The authors would like to thank the Forest Service of Sardinia for collaborating in this study. This work was partially funded by the GEMINA Project-MIUR/MATTM n. 232/2011, by the FUME Project-FP7/2007-2013, Grant Agreement 243888, and by the Project "Modeling approach to evaluate fire risk and mitigation planning actions'' (P.O.R. SARDEGNA F. S. E. 2007-2013, Asse IV Capitale umano, Linea di Attivita l. 3.1). NR 103 TC 27 Z9 27 U1 1 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0921-030X EI 1573-0840 J9 NAT HAZARDS JI Nat. Hazards PD APR PY 2014 VL 71 IS 3 BP 1389 EP 1418 DI 10.1007/s11069-013-0951-0 PG 30 WC Geosciences, Multidisciplinary; Meteorology & Atmospheric Sciences; Water Resources SC Geology; Meteorology & Atmospheric Sciences; Water Resources GA AB8FJ UT WOS:000332025200008 ER PT J AU Li, H Lee, WS Wang, K Ehsani, R Yang, CH AF Li, Han Lee, Won Suk Wang, Ku Ehsani, Reza Yang, Chenghai TI 'Extended spectral angle mapping (ESAM)' for citrus greening disease detection using airborne hyperspectral imaging SO PRECISION AGRICULTURE LA English DT Article DE Image classification; Precision agriculture; Red-edge position; Spectral angle mapping; Vertex component analysis ID REMOTE-SENSING DATA; RED EDGE POSITION; REFLECTANCE; CLASSIFICATION; ALGORITHM; IMAGERY; YIELD AB Hyperspectral (HS) imaging is becoming more important for agricultural applications. Due to its high spectral resolution, it exhibits excellent performance in disease identification of different crops. In this study, a novel method termed 'extended spectral angle mapping (ESAM)' was proposed to detect citrus greening disease (Huanglongbing or HLB), which is a very destructive disease of citrus. Firstly, the Savitzky-Golay smoothing filter was used to remove spectral noise within the data. A mask for tree canopy was built using support vector machine, to separate the tree canopies from the background. Pure endmembers of the masked dataset for healthy and HLB infected tree canopies were extracted using vertex component analysis. By utilizing the derived pure endmembers, spectral angle mapping was applied to differentiate between healthy and citrus greening disease infected areas in the image. Finally, most false positive detections were filtered out using red-edge position. An experiment was carried out using an HS image acquired by an airborne HS imaging system, and a multispectral image acquired by the WorldView-2 satellite, from the Citrus Research and Education Center, Lake Alfred, FL, USA. Ground reflectance measurement and coordinates for diseased trees were recorded. The experimental results were compared with another supervised method, Mahalanobis distance, and an unsupervised method, K-means, both of which showed a 63.6 % accuracy. The proposed ESAM performed better with a detection accuracy of 86 % than those two methods. These results demonstrated that the detection accuracy using HS image could be enhanced by focusing on the pure endmember extraction and the use of red-edge position, suggesting that there is a great potential of citrus greening disease detection using an HS image. C1 [Li, Han; Wang, Ku] China Agr Univ, Dept Informat & Elect Engn, Beijing 100083, Peoples R China. [Lee, Won Suk] Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. [Ehsani, Reza] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA. [Yang, Chenghai] USDA ARS, Southern Plains Agr Res Ctr, College Stn, TX USA. RP Lee, WS (reprint author), Univ Florida, Dept Agr & Biol Engn, Gainesville, FL 32611 USA. EM wslee@ufl.edu FU Citrus Research and Development Foundation, Inc.; China Scholarship Council FX This project was funded by the Citrus Research and Development Foundation, Inc. The authors would like to thank Ms. Ce Yang, Ms. Sherrie Buchanon, Ms. Lioubov Polonik, Mr. Anurag R. Katti, Mr. Alireza Pourreza, and Mr. Junsu Shin at the University of Florida for their assistance in this study. The authors also would like to thank the China Scholarship Council for financial support. NR 33 TC 21 Z9 21 U1 5 U2 58 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1385-2256 EI 1573-1618 J9 PRECIS AGRIC JI Precis. Agric. PD APR PY 2014 VL 15 IS 2 SI SI BP 162 EP 183 DI 10.1007/s11119-013-9325-6 PG 22 WC Agriculture, Multidisciplinary SC Agriculture GA AC3WX UT WOS:000332453200003 ER PT J AU Anderson, A Shwiff, S Gebhardt, K Ramirez, AJ Shwiff, S Kohler, D Lecuona, L AF Anderson, A. Shwiff, S. Gebhardt, K. Ramirez, A. J. Shwiff, S. Kohler, D. Lecuona, L. TI Economic Evaluation of Vampire Bat (Desmodus rotundus) Rabies Prevention in Mexico SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE benefit-cost analysis; vampire bat (Desmodus rotundus); economics; Mexico; cattle; rabies ID VACCINATION; OUTBREAK AB Vampire bat rabies causes significant impacts within its endemic range in Mexico. These impacts include livestock mortality, animal testing costs, post-exposure prophylaxis costs, and human mortality risk. Mitigation of the impacts can be achieved by vaccinating livestock and controlling vampire bat populations. A benefit-cost analysis was performed to examine the economic efficiency of these methods of mitigation, and Monte Carlo simulations were used to examine the impact that uncertainty has on the analysis. We found that livestock vaccination is efficient, with benefits being over six times higher than costs. However, bat control is inefficient because benefits are very unlikely to exceed costs. It is concluded that when these mitigation methods are judged by the metric of economic efficiency, livestock vaccination is desirable but bat control is not. C1 [Anderson, A.; Shwiff, S.; Gebhardt, K.; Kohler, D.] USDA, WS, Natl Wildlife Res Ctr, Ft Collins, CO USA. [Shwiff, S.] Texas A&M Univ Commerce, Commerce, TX USA. [Lecuona, L.] USDA APHIS IS NAR Mexico, Mexico City, DF, Mexico. RP Anderson, A (reprint author), Natl Wildlife Res Ctr, Res Inst, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM aaron.m.anderson@aphis.usda.gov NR 18 TC 5 Z9 5 U1 4 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD APR PY 2014 VL 61 IS 2 BP 140 EP 146 DI 10.1111/tbed.12007 PG 7 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA AB9YT UT WOS:000332152300006 PM 22984914 ER PT J AU Ketusing, N Reeves, A Portacci, K Yano, T Olea-Popelka, F Keefe, T Salman, M AF Ketusing, N. Reeves, A. Portacci, K. Yano, T. Olea-Popelka, F. Keefe, T. Salman, M. TI Evaluation of Strategies for the Eradication of Pseudorabies Virus (Aujeszky's Disease) in Commercial Swine Farms in Chiang-Mai and Lampoon Provinces, Thailand, Using a Simulation Disease Spread Model SO TRANSBOUNDARY AND EMERGING DISEASES LA English DT Article DE Pseudorabies; eradication strategies; simulation model AB Several strategies for eradicating Pseudorabies virus (Aujeszky's disease) in Chiang-Mai and Lampoon Provinces, Thailand, were compared using a computer simulation model, the North American Animal Disease Spread Model (NAADSM). The duration of the outbreak, the number of affected herds and the number of destroyed herds were compared during these simulated outbreaks. Depopulation, zoning for restricted movement and improved detection and vaccination strategies were assessed. The most effective strategies to eradicate Pseudorabies as per the findings from this study are applying depopulation strategies with MOVEMENT RESTRICTIONS in 3-, 8- and 16-km ZONES surrounding infected herds and enhancing the eradication with vaccination campaign on 16-km radius surrounding infected herds. C1 [Ketusing, N.] Natl Inst Anim Hlth, Dept Livestock Dev, Bangkok 10900, Thailand. [Reeves, A.; Olea-Popelka, F.; Salman, M.] Colorado State Univ, Coll Vet Med & Biomed Sci, Anim Populat Hlth Inst, Ft Collins, CO 80523 USA. [Portacci, K.] Vet Serv, USDA, Anim Plant Hlth Inspect Serv, Ctr Epidemiol & Anim Hlth, Ft Collins, CO USA. [Yano, T.] Chiang Mai Univ, Fac Vet Med, Chiang Mai 50000, Thailand. [Keefe, T.] Colorado State Univ, Ft Collins, CO 80523 USA. RP Ketusing, N (reprint author), Natl Inst Anim Hlth, Dept Livestock Dev, Bangkok 10900, Thailand. EM naree.ket@gmail.com OI Reeves, Aaron/0000-0001-6387-7481 NR 9 TC 4 Z9 5 U1 7 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1865-1674 EI 1865-1682 J9 TRANSBOUND EMERG DIS JI Transbound. Emerg. Dis. PD APR PY 2014 VL 61 IS 2 BP 169 EP 176 DI 10.1111/tbed.12017 PG 8 WC Infectious Diseases; Veterinary Sciences SC Infectious Diseases; Veterinary Sciences GA AB9YT UT WOS:000332152300010 PM 23033968 ER PT J AU Spencer, DF Enloe, SF Pitcairn, MJ Ditomaso, JM AF Spencer, D. F. Enloe, S. F. Pitcairn, M. J. Ditomaso, J. M. TI Impacts of mowing and bud destruction on Centaurea solstitialis growth, flowering, root dynamics and soil moisture SO WEED RESEARCH LA English DT Article DE yellow starthistle; mechanical control; biological control; biomass allocation; reproduction; root persistence ID YELLOW STARTHISTLE; BIOLOGICAL-CONTROL; CALIFORNIA; RANGELAND; COLEOPTERA; FECUNDITY; LOSSES AB Centaurea solstitialis (yellow starthistle) has invaded rangelands and natural areas in the western USA. There is evidence that mowing is a more effective method of reducing growth and seed production, compared with the well-established and distributed bioagents. To test this hypothesis we measured C.solstitialis's growth and reproduction following two treatments (late spring and early summer bud/capitula destruction and shoot mowing) and measured C.solstitialis's growth and reproduction. We measured changes in soil moisture content to test the hypothesis that these manipulations differentially affect water usage by the invasive plant. Mowing produced shorter plants that weighed less. Bud damaged plants were not statistically different from untreated controls. The number of developed capitula was reduced 67% by mowing but was unaffected by bud damage. Mowed plants redistributed resources from root to flower production. Both treatments reduced mean capitulum diameter. This resulted in reductions of 76% and 21% in estimated seed number for mowed and bud damaged plants respectively. Root abundance decreased and root life-span was reduced by both treatments. Soil moisture depletion was greatest from mid-May to mid-July (from 21% to 9%) and occurred after maximum root abundance. Mowing resulted in a delay in soil moisture depletion compared with the bud damaged plants or the untreated controls. Bud/capitula damage did not affect C.solstitialis's requirement for soil water. Overall, this study supported the hypothesis that mowing reduced C.solstitialis growth and reproduction more than bud damage alone. This information will aid managers in selecting a strategy for managing C.solstitialis in a particular habitat. C1 [Spencer, D. F.] ARS, USDA, Exot & Invas Weeds Res Unit, Davis, CA 95616 USA. [Enloe, S. F.] Auburn Univ, Dept Agron & Soils, Auburn, AL 36849 USA. [Pitcairn, M. J.] Calif Dept Food & Agr, Sacramento, CA 95814 USA. [Ditomaso, J. M.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Spencer, DF (reprint author), ARS, Dept Plant Sci, USDA, Exot & Invas Weeds Res Unit, Mail Stop 4,One Shields Ave, Davis, CA 95616 USA. EM david.spencer@ars.usda.gov NR 34 TC 1 Z9 1 U1 2 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0043-1737 EI 1365-3180 J9 WEED RES JI Weed Res. PD APR PY 2014 VL 54 IS 2 BP 140 EP 150 DI 10.1111/wre.12058 PG 11 WC Agronomy; Plant Sciences SC Agriculture; Plant Sciences GA AB8WU UT WOS:000332071500004 ER PT J AU Baranowski, T AF Baranowski, Tom TI Descriptions for Articles Introducing a New Game for Health SO GAMES FOR HEALTH JOURNAL LA English DT Editorial Material C1 Baylor Univ, USDA ARS, Childrens Nutr Res Ctr,Dept Pediat, Pediat Behav Nutr & Phys Act,Baylor Coll Med, Houston, TX 77030 USA. RP Baranowski, T (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr,Dept Pediat, Pediat Behav Nutr & Phys Act,Baylor Coll Med, Houston, TX 77030 USA. OI Baranowski, Tom/0000-0002-0653-2222 NR 0 TC 4 Z9 4 U1 0 U2 0 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD APR PY 2014 VL 3 IS 2 BP 55 EP 56 DI 10.1089/g4h.2014.0008 PG 2 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CU2VP UT WOS:000363382000001 PM 26196042 ER PT J AU Baranowski, T Maddison, R Maloney, A Medina, E Simons, M AF Baranowski, Tom Maddison, Ralph Maloney, Ann Medina, Ernie, Jr. Simons, Monique TI Building a Better Mousetrap (Exergame) to Increase Youth Physical Activity SO GAMES FOR HEALTH JOURNAL LA English DT Article ID SELF-DETERMINATION THEORY; VIDEO GAMES; BEHAVIOR-CHANGE; OVERWEIGHT CHILDREN; WEIGHT-LOSS; HEALTH; MOTIVATION; EDUCATION; COMPUTER; DIETARY AB Although exergames have been demonstrated to induce moderate levels of physical activity (PA) if played as designed, there is conflicting evidence on use of exergaming leading to increased habitual PA. Exergames have increased PA in some home and school studies, but not others. Exergames have been used in community centers to good effect, but this has not generally been validated with research. PA from exergames may be enhanced by innovative use of sensors, "fun''-enhancing procedures, tailored messaging, message framing, story or narrative, goal setting, feedback, and values-based messaging. Research is needed on PA-enhancing procedures used within exergames for youth to provide a firmer foundation for the design and use of exergames in the future. C1 [Baranowski, Tom] Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, Houston, TX 77030 USA. [Maddison, Ralph] Univ Auckland, Natl Inst Hlth Innovat, Auckland 1, New Zealand. [Maloney, Ann] Univ Massachusetts, Worcester, MA 01605 USA. [Medina, Ernie, Jr.] Loma Linda Univ, Sch Publ Hlth, Ctr Nutr Hlth Lifestyles & Dis Prevent, Loma Linda, CA 92350 USA. [Simons, Monique] Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Hlth Sci, Amsterdam, Netherlands. [Simons, Monique] Vrije Univ Amsterdam, Fac Earth & Life Sci, EMGO Inst Hlth & Care Res, Amsterdam, Netherlands. [Simons, Monique] Vrije Univ Amsterdam, Med Ctr, TNO VU VUmc, Body Work,Res Ctr Phys Act Work & Hlth, Amsterdam, Netherlands. [Simons, Monique] TNO, Expertise Ctr Life Style, Leiden, Netherlands. RP Baranowski, T (reprint author), Baylor Univ, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat,Baylor Coll Med, 1100 Bates St, Houston, TX 77030 USA. EM tbaranow@bcm.edu OI Baranowski, Tom/0000-0002-0653-2222; Maddison, Ralph/0000-0001-8564-5518 NR 69 TC 12 Z9 12 U1 0 U2 1 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 2161-783X EI 2161-7856 J9 GAMES HEALTH J JI Games Health J. PD APR PY 2014 VL 3 IS 2 BP 72 EP 78 DI 10.1089/g4h.2014.0018 PG 7 WC Health Policy & Services; Public, Environmental & Occupational Health; Rehabilitation SC Health Care Sciences & Services; Public, Environmental & Occupational Health; Rehabilitation GA CU2VP UT WOS:000363382000005 PM 26196047 ER PT J AU Berger, K Massolo, A Zuliani, A Freier, J Linthicum, KJ Cork, S AF Berger, K. Massolo, A. Zuliani, A. Freier, J. Linthicum, K. J. Cork, S. TI Spatio-temporal patterns of epizootic hemorrhagic disease (EHD) occurrence in the continental USA (1980-2010) SO INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES LA English DT Meeting Abstract C1 [Berger, K.; Massolo, A.; Zuliani, A.; Cork, S.] Univ Calgary, Fac Vet Med, Calgary, AB, Canada. [Freier, J.] USDA APHIS Vet Serv, Ft Collins, CO USA. [Linthicum, K. J.] USDA, Gainesville, FL USA. RI Massolo, Alessandro/I-3437-2012 NR 0 TC 0 Z9 0 U1 1 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1201-9712 EI 1878-3511 J9 INT J INFECT DIS JI Int. J. Infect. Dis. PD APR PY 2014 VL 21 SU 1 MA 47.036 BP 193 EP 193 DI 10.1016/j.ijid.2014.03.823 PG 1 WC Infectious Diseases SC Infectious Diseases GA V43TP UT WOS:000209704000408 ER PT J AU Gao, F Anderson, MC Kustas, WP Houborg, R AF Gao, Feng Anderson, Martha C. Kustas, William P. Houborg, Rasmus TI Retrieving Leaf Area Index From Landsat Using MODIS LAI Products and Field Measurements SO IEEE GEOSCIENCE AND REMOTE SENSING LETTERS LA English DT Article DE Biophysical parameters; disaggregation; downscaling; Landsat; leaf area index (LAI); Moderate Resolution Imaging Spectroradiometer (MODIS); Soil Moisture Experiment of 2002 (SMEX02) field campaign; spatial statistics ID MAPPING DAILY EVAPOTRANSPIRATION; VALIDATION; IMAGERY; SCALES; TERRA AB Land surface models that operate at multiple spatial resolutions require consistent leaf area index (LAI) inputs at each scale. In order to produce LAI from Landsat imagery that is consistent with the Moderate Resolution Imaging Spectroradiometer (MODIS) LAI product and with in situ measurements, an improved regression tree mapping approach has been developed, which uses homogeneous and high-quality LAI retrievals from MODIS and existing LAI measurements acquired on the ground as samples to train a rule-based model based on Landsat surface reflectance data in visible and infrared bands. The methodology employs a simple geostatistical approach for determining sample weight, considering the spatial distribution of the samples and data quality. Results from the Soil Moisture Experiment of 2002 field campaign show that incorporation of available ground-based LAI measurements into the training sample collection improved field-scale estimation of LAI, especially in areas of very high vegetation cover fraction, but did not degrade consistency with MODIS LAI products. C1 [Gao, Feng; Anderson, Martha C.; Kustas, William P.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Houborg, Rasmus] European Commis, JRC, Inst Environm & Sustainabil, I-21027 Ispra, Italy. RP Gao, F (reprint author), ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. EM feng.gao@ars.usda.gov; martha.anderson@ars.usda.gov; bill.kustas@ars.usda.gov RI Anderson, Martha/C-1720-2015; Kustas, William/C-2063-2015 OI Anderson, Martha/0000-0003-0748-5525; FU U.S. Geological Survey Landsat Science Team program; National Aeronautics and Space Administration Terrestrial Ecology program FX This work was supported in part by the U.S. Geological Survey Landsat Science Team program and the National Aeronautics and Space Administration Terrestrial Ecology program. NR 20 TC 6 Z9 8 U1 2 U2 39 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1545-598X EI 1558-0571 J9 IEEE GEOSCI REMOTE S JI IEEE Geosci. Remote Sens. Lett. PD APR PY 2014 VL 11 IS 4 BP 773 EP 777 DI 10.1109/LGRS.2013.2278782 PG 5 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA AC0KP UT WOS:000332183500012 ER PT J AU Kim, Y Jackson, T Bindlish, R Hong, S Jung, G Lee, K AF Kim, Yihyun Jackson, Thomas Bindlish, Rajat Hong, Sukyoung Jung, Gunho Lee, Kyuongdo TI Retrieval of Wheat Growth Parameters With Radar Vegetation Indices SO IEEE GEOSCIENCE AND REMOTE SENSING LETTERS LA English DT Article DE Backscattering coefficients; fresh weight; polarimetric scatterometer; radar vegetation index (RVI); vegetation water content (VWC) ID LEAF-AREA INDEX; BACKSCATTERING COEFFICIENT; MICROWAVE BACKSCATTERING; WATER-CONTENT; SAR DATA; RICE; IMAGERY; SCATTEROMETER; COVERS; CROPS AB The radar vegetation index (RVI) has low sensitivity to changes in environmental conditions and has the potential as a tool to monitor vegetation growth. In this letter, we expand on previous research by investigating the radar response over a wheat canopy. RVI was computed using observations made with a ground-based multifrequency polarimetric scatterometer system over an entire wheat growth cycle. We analyzed the temporal variations of backscattering coefficients for L-, C-, and X-bands; RVI; vegetation water content (VWC); and fresh weight. We found that the L-band RVI was highly correlated with both VWC (r = 0.98) and fresh weight (r = 0.98). Based upon these analyses, linear equations were developed for estimation of VWC (root-mean-square error (RMSE = 0.126 kg m(-2))) and fresh weight (RMSE = 0.12 kg m(-2)). In addition, the results of the wheat study were combined with previous investigations with other crops (e. g., rice and soybean). We found that a single linear relationship between L-band RVI and VWC can be used for all crop types (RMSE = 0.47kg m(-2)). These results clearly demonstrate the potential of RVI as a robust method for characterizing vegetation canopies. VWC is a key input requirement for retrieving soil moisture from microwave remote sensing observations. The results of this investigation will be useful for the Soil Moisture Active and Passive mission (2014), which is designed to measure global soil moisture. C1 [Kim, Yihyun; Hong, Sukyoung; Lee, Kyuongdo] Rural Dev Adm, Natl Acad Agr Sci, Suwon 441707, South Korea. [Jackson, Thomas; Bindlish, Rajat] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Jung, Gunho] Rural Dev Adm, Natl Inst Crop Sci, Suwon 441857, South Korea. RP Kim, Y (reprint author), Rural Dev Adm, Natl Acad Agr Sci, Suwon 441707, South Korea. FU Rural Development Administration, Korea [PJ009367012013] FX This work was supported by the Agenda Program of the Rural Development Administration, Korea, under Grant PJ009367012013. NR 27 TC 12 Z9 13 U1 3 U2 45 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1545-598X EI 1558-0571 J9 IEEE GEOSCI REMOTE S JI IEEE Geosci. Remote Sens. Lett. PD APR PY 2014 VL 11 IS 4 BP 808 EP 812 DI 10.1109/LGRS.2013.2279255 PG 5 WC Geochemistry & Geophysics; Engineering, Electrical & Electronic; Remote Sensing; Imaging Science & Photographic Technology SC Geochemistry & Geophysics; Engineering; Remote Sensing; Imaging Science & Photographic Technology GA AC0KP UT WOS:000332183500019 ER PT J AU Haznadar, M Maruvada, P Mette, E Milner, J Moore, SC Nicastro, HL Sampson, JN Su, LJ Verma, M Zanetti, KA AF Haznadar, Majda Maruvada, Padma Mette, Eliza Milner, John Moore, Steven C. Nicastro, Holly L. Sampson, Joshua N. Su, L. Joseph Verma, Mukesh Zanetti, Krista A. TI Navigating the road ahead: addressing challenges for use of metabolomics in epidemiology studies SO METABOLOMICS LA English DT Article ID CANCER RISK C1 [Haznadar, Majda] NCI, Ctr Canc Res, Bethesda, MD 20892 USA. [Maruvada, Padma] Natl Inst Diabet & Digest & Kidney Dis, Div Digest Dis & Nutr, Bethesda, MD 20817 USA. [Mette, Eliza; Su, L. Joseph; Verma, Mukesh; Zanetti, Krista A.] NCI, Div Canc Control & Populat Sci, Rockville, MD 20892 USA. [Milner, John] ARS, USDA, Beltsville, MD 20705 USA. [Moore, Steven C.; Sampson, Joshua N.] NCI, Div Canc Epidemiol & Genet, Rockville, MD 20892 USA. [Nicastro, Holly L.] NCI, Canc Prevent Div, Rockville, MD 20892 USA. RP Zanetti, KA (reprint author), NCI, Div Canc Control & Populat Sci, Rockville, MD 20892 USA. EM zanettik@mail.nih.gov RI Moore, Steven/D-8760-2016 OI Moore, Steven/0000-0002-8169-1661 FU Intramural NIH HHS [Z99 HL999999] NR 8 TC 3 Z9 3 U1 4 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1573-3882 EI 1573-3890 J9 METABOLOMICS JI Metabolomics PD APR PY 2014 VL 10 IS 2 BP 176 EP 178 DI 10.1007/s11306-014-0636-z PG 3 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AB7YJ UT WOS:000332007000001 PM 25914611 ER PT J AU Vilanova, L Wisniewski, M Norelli, J Vinas, I Torres, R Usall, J Phillips, J Droby, S Teixido, N AF Vilanova, L. Wisniewski, M. Norelli, J. Vinas, I. Torres, R. Usall, J. Phillips, J. Droby, S. Teixido, N. TI Transcriptomic Profiling of Apple in Response to Inoculation with a Pathogen (Penicillium expansum) and a Non-pathogen (Penicillium digitatum) SO PLANT MOLECULAR BIOLOGY REPORTER LA English DT Article DE Green mold; Bluemold; Maturity stage; Microarray analysis; Gene expression; Phenylpropanoid pathway ID PHENYLALANINE AMMONIA-LYASE; INDUCED CELL-DEATH; GENE-EXPRESSION; CITRUS-FRUIT; DISEASE RESISTANCE; HYDROGEN-PEROXIDE; BOTRYTIS-CINEREA; OXIDATIVE BURST; ARABIDOPSIS-THALIANA; MICROARRAY ANALYSIS AB Penicillium expansum, the causal agent of blue mould of pome fruits, is a major postharvest pathogen in all producing countries. To develop a better understanding of disease resistance mechanisms in apples, a comprehensive transcriptional analysis of apple gene expression in response to a compatible (P. expansum) and non-host (Penicillium digitatum) pathogen was conducted using an apple microarray of approximately 40,000 probes. The resulting data provide further evidence that apples inoculated with P. expansum exhibit significant upregulation of defense-related genes and genes involved in detoxification of reactive oxygen species. In contrast, apples inoculated with P. digitatum, a non-host pathogen, exhibited upregulation of genes involved in phenylpropanoid metabolism. To confirm the accuracy of the expression profiles obtained with the microarray, reverse transcriptase-quantitative polymerase chain reaction was conducted for four genes specifically in the phenylpropanoid pathway. Expression data was obtained for different time points and fruit maturity stages. The highest expression level of the phenylpropanoid genes was detected 48 h after inoculation with P. expansum in both immature and mature apples. These results support the hypothesis that apples respond in a complex and diverse manner to the compatible compared to the non-host pathogen. To the best of our knowledge, this is the first study in apple fruit that has conducted an analysis of global changes in gene expression in response to a compatible (P. expansum) and non-host (P. digitatum) pathogen. C1 [Vilanova, L.; Torres, R.; Usall, J.; Teixido, N.] IRTA, XaRTA Postharvest, Lleida 25198, Catalonia, Spain. [Wisniewski, M.; Norelli, J.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Vinas, I.] Lleida Univ, Dept Food Technol, Agrotecnio Ctr, XaRTA Postharvest, Lleida 25198, Catalonia, Spain. [Phillips, J.] USDA ARS, Eastern Reional Res Ctr, Wyndmoor, PA USA. [Droby, S.] Agr Res Org, Volcani Ctr, Dept Postharvest Sci, IL-50250 Bet Dagan, Israel. RP Teixido, N (reprint author), IRTA, XaRTA Postharvest, Rovira Roure 191, Lleida 25198, Catalonia, Spain. EM neus.teixido@irta.cat RI VILANOVA, LAURA/F-5531-2010; Teixido, Neus/D-2487-2017; OI VILANOVA, LAURA/0000-0001-7574-9604; Teixido, Neus/0000-0002-1676-3593; Vinas, Inmaculada/0000-0001-5182-2520 FU Spanish Government (Plan Nacional de I+D+I, Ministerio de Ciencia e Innovacion, Spain) [AGL2008-04828-C03/AGR, AGL2011-30519-C03/AGR]; "Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria" (INIA) FX The authors would like to thank Erik Burchard for his excellent technical assistance in all aspects of this study and Dr. Svetlana Dashevskaya for her help in RT-qPCR analysis. The authors are grateful to the Spanish Government for financial support by two national projects AGL2008-04828-C03/AGR and AGL2011-30519-C03/AGR (Plan Nacional de I+D+I, Ministerio de Ciencia e Innovacion, Spain), and the "Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria" (INIA) for L. Vilanova PhD grant. NR 66 TC 11 Z9 11 U1 3 U2 39 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0735-9640 EI 1572-9818 J9 PLANT MOL BIOL REP JI Plant Mol. Biol. Rep. PD APR PY 2014 VL 32 IS 2 BP 566 EP 583 DI 10.1007/s11105-013-0676-y PG 18 WC Biochemical Research Methods; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AB3RS UT WOS:000331708500023 ER PT J AU Lulai, EC Neubauer, JD AF Lulai, Edward C. Neubauer, Jonathan D. TI Wound-induced suberization genes are differentially expressed, spatially and temporally, during closing layer and wound periderm formation SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Closing layer; Potato tuber; Solanum tuberosum L; Suberization; Wound-heal; Wound periderm ID PHENYLALANINE AMMONIA-LYASE; POTATO SOLANUM-TUBEROSUM; ABSCISIC-ACID; ANIONIC PEROXIDASE; HEALING ABILITY; SUBERIN; ARABIDOPSIS; DEPOSITION; RESISTANCE; INDUCTION AB Potato tuber (Solanum tuberosum L) wounds incurred at harvest and upon seed cutting require rapid suberization as a major part of the healing process to prevent infection and desiccation. However, little is known about the induction and expression of genes that are essential for these processes and in particular to the two major stages of wound-induced suberization, i.e. closing layer formation and wound periderm formation. The objectives of this research were to address these needs by determining the effects of wounding on the induction and expression profiles of specific genes involved in wound-induced suberization in potato tuber (S. tuberosum L) during the initiation and completion of closing layer formation and wound periderm formation. Although both stages critically involve suberization, there are significant differences between the two processes. Closing layer development requires rapid suberization of existing parenchyma cells bordering the wound surface to provide the initial protective barrier for the wound. Wound periderm development occurs later, i.e. after completion of closing layer formation, and requires development of a wound phellogen layer which mediates the formation of highly organized files of suberized wound-phellem cells that provide a more durable protective barrier for the tuber. The processes delineating these two separate stages of wound-induced suberization are poorly understood. This research shows that, unlike some wound responding genes such as phenylalanine ammonia lyase (StPAL-1) and anionic peroxidase (StPrx), certain genes that are specifically involved in both of these processes do not remain uniformly up-regulated during the two stages of healing (i.e. StTHT encoding Hydroxycinnamoyl-CoA:tyramine N-(hydroxycinnamoyl)transferase, StFHT encoding a fatty omega-hydroxyacid/fatty alcohol hydroxycinnamoyl transferase, StKCS6 encoding a 3-ketoacyl-CoA synthase, StFAOH encoding a fatty acid omega-hydroxylase and StGPAT5 encoding a protein with acyl-CoA:glycerol-3-phosphate acyltransferase). Instead, they are up-regulated during closing layer formation; i.e. starting by ca. 1 d after wounding, but then slightly down-regulated or pause near completion of the closing layer (ca. 5-6 d) and then again up-regulated as wound periderm development is fully initiated (ca. 7 d) and down-regulated near completion (ca. 28 d after wounding). This differential in the expression profile, i.e. decrease between stages, was not anticipated and may be the first demonstration of measurable changes of any sort of biological flux as wound induced suberization transitions from closing layer to wound periderm development. Results were repeated using minitubers from two different crop years and demonstrate that these processes are separate, but coupled in some yet to be determined fashion. The biology of this differential expression is important because of the roles closing layer and wound periderm development play in protecting the tuber from disease and other challenges. (C) 2013 Published by Elsevier B.V. C1 [Lulai, Edward C.; Neubauer, Jonathan D.] USDA ARS, No Crop Sci Lab, Fargo, ND 58102 USA. RP Lulai, EC (reprint author), USDA ARS, No Crop Sci Lab, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM ed.lulai@ars.usda.gov NR 35 TC 8 Z9 9 U1 6 U2 31 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD APR PY 2014 VL 90 BP 24 EP 33 DI 10.1016/j.postharvbio.2013.11.010 PG 10 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AB5SY UT WOS:000331850200004 ER EF