FN Thomson Reuters Web of Science™ VR 1.0 PT J AU McPherson, EG Kendall, A AF McPherson, E. Gregory Kendall, Alissa TI A life cycle carbon dioxide inventory of the Million Trees Los Angeles program SO INTERNATIONAL JOURNAL OF LIFE CYCLE ASSESSMENT LA English DT Article DE Carbon footprint; Carbon sequestration; Climate change; Life cycle inventory; Tree planting; Urban forestry; Urban trees ID URBAN; DECOMPOSITION; LITTER; PATTERNS; BIOMASS; SYSTEM; CITIES AB This study seeks to answer the question, "Will the Million Trees LA (Million Trees Los Angeles, MTLA) program be a carbon dioxide (CO2) sink or source?" Because there has never been a full accounting of CO2 emissions, it is unclear if urban tree planting initiatives (TPIs) are likely to be effective means for reaching local reduction targets. Using surveys, interviews, field sampling, and computer simulation of tree growth and survival over a 40-year time period, we developed the first process-based life cycle inventory of CO2 for a large TPI. CO2 emissions and reductions from storage and avoided emissions from energy savings were simulated for 91,786 trees planted from 2006 to 2010, of which only 30,813 (33.6 %) were estimated to survive. The MTLA program was estimated to release 17,048 and 66,360 t of fossil and biogenic CO2 over the 40-year period, respectively. The total amount emitted (83,408 t) was slightly more than the -77,942 t CO2 that trees were projected to store in their biomass. The MTLA program will be a CO2 sink if projected 40-year-avoided fossil fuel CO2 emissions from energy savings (-101,679 t) and biopower (-1,939 t) are realized. The largest sources of CO2 emissions were mulch decomposition (65.1 %), wood combustion (14.5 %), and irrigation water (9.7 %). Although trees planted by the MTLA program are likely to be a net CO2 sink, there is ample opportunity to reduce emissions. Examples of these opportunities include selecting drought-tolerant trees and utilizing wood residue to generate electricity rather than producing mulch. C1 [McPherson, E. Gregory] US Forest Serv, Urban Ecosyst & Social Dynam Program, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Kendall, Alissa] Univ Calif Davis, Dept Civil & Environm Engn, Davis, CA 95616 USA. RP McPherson, EG (reprint author), US Forest Serv, Urban Ecosyst & Social Dynam Program, Pacific Southwest Res Stn, USDA, 1731 Res Pk Dr, Davis, CA 95618 USA. EM gmcpherson@fs.fed.us NR 49 TC 7 Z9 9 U1 2 U2 39 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0948-3349 EI 1614-7502 J9 INT J LIFE CYCLE ASS JI Int. J. Life Cycle Assess. PD SEP PY 2014 VL 19 IS 9 BP 1653 EP 1665 DI 10.1007/s11367-014-0772-8 PG 13 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AN5HN UT WOS:000340621500008 ER PT J AU van den Berg, AK Perkins, TD Isselhardt, ML Godshall, MA Lloyd, SW AF van den Berg, Abby K. Perkins, Timothy D. Isselhardt, Mark L. Godshall, Mary An Lloyd, Steven W. TI Effects of membrane separation on maple syrup composition and flavor SO INTERNATIONAL SUGAR JOURNAL LA English DT Article DE maple syrup; maple sap; membrane separation; sugar maple; evaporator ID CHEMICAL-COMPOSITION AB Concentration of maple sap by membrane separation prior to thermal evaporation significantly increases the profitability of maple syrup production by reducing the amount of fuel and time required to concentrate the material to syrup density. While several studies have suggested the practice does not significantly impact the quality of the maple syrup produced, thus far there have been no direct comparisons of syrup produced from raw, non-concentrated maple sap with syrup made from the same pool of sap concentrated by membrane separation. The objective of this study was thus to determine if the chemical composition and flavor of syrup produced from raw sap differed significantly from syrup produced simultaneously from the same sap concentrated to 8 degrees Brix by membrane separation. Maple syrup produced from raw maple sap was slightly, but significantly lighter in color than syrup produced simultaneously from the same sap concentrated to 8 degrees Brix (p < 0.0001). There were few other differences in the composition or properties of the two types of syrup, and no differences were detected in the relative quantities of volatile flavor compounds or in the perceived flavor of the two. Though the darker-colored syrup produced with concentrated sap would result in a slight loss of economic value, this would be more than compensated for by the reduced processing costs associated with the use of membrane separation. These results indicate that producing syrup with sap concentrated by membrane separation does not significantly impact syrup quality, and thus that this is an acceptable practice producers can use to increase the profitability of maple syrup production. C1 [van den Berg, Abby K.; Perkins, Timothy D.; Isselhardt, Mark L.] Univ Vermont, Proctor Maple Res Ctr, Underhill Ctr, VT 05490 USA. [Godshall, Mary An] Sugar Proc Res Inst Inc, New Orleans, LA 70124 USA. [Lloyd, Steven W.] ARS, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP van den Berg, AK (reprint author), Univ Vermont, Proctor Maple Res Ctr, POB 233, Underhill Ctr, VT 05490 USA. EM Abby.vandenBerg@uvm.edu RI van den Berg, Abby/N-1829-2013 FU United States Department of Agriculture National Institute of Food and Agriculture (USDA NIFA) grant [2010-34157-21008] FX This work was supported by United States Department of Agriculture National Institute of Food and Agriculture (USDA NIFA) grant 2010-34157-21008. The authors would like to thank Eric Sorkin, Marianne McKee, Brian Perkins, Ben Dana, Ted Ortiz Y Pino, Teague Henkle, and Brian Stowe for their assistance with this research. NR 17 TC 0 Z9 0 U1 5 U2 11 PU INT SUGAR JOURNAL LTD PI KENT PA 80 CALVERLEY, TUNBRIDGE WELLS, KENT TN1 2UN, WALES SN 0020-8841 J9 INT SUGAR J JI Int. Sugar J. PD SEP PY 2014 VL 116 IS 1389 BP 656 EP 665 PG 10 WC Agronomy; Food Science & Technology SC Agriculture; Food Science & Technology GA AO4DO UT WOS:000341286600036 ER PT J AU Musgrove, MT Stephens, CB Bourassa, DV Cox, NA Mauldin, JM Berrang, ME Buhr, RJ AF Musgrove, M. T. Stephens, C. B. Bourassa, D. V. Cox, N. A. Mauldin, J. M. Berrang, M. E. Buhr, R. J. TI Enterobacteriaceae and Salmonella recovered from nonsanitized and sanitized broiler hatching eggs SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE hatchery; microbiology; eggs; sanitation; sanitizer ID REDUCING AIRBORNE PATHOGENS; SPACE-CHARGE SYSTEM; SHELL CRUSH METHODS; BACTERICIDAL TREATMENT; ELIMINATE SALMONELLA; CHICKEN OPERATIONS; TYPHIMURIUM; HATCHERIES; CHEMICALS; POULTRY AB Sanitizing hatching eggs may reduce the chances that a broiler flock will become colonized with Salmonella and reduce the numbers of other microorganisms, such as Enterobacteriaceae, that can depress hatchability. An experiment was conducted to determine if a quaternary-biguanide sanitizer applied as foam or spray would reduce Enterobacteriaceae or Salmonella naturally occurring on broiler hatching eggs. The sanitizer was applied to buggies of 5,040 eggs the day before set (one buggy/treatment at each of 2 settings). Treated eggs were compared with untreated controls. Foam application lowered Enterobacteriaceae prevalence at set (0 vs. 18%) and transfer (5 vs. 28%); spraying was effective only when eggs were set (2.5 vs. 11%). At transfer spray, treated and control eggs were 19% Enterobacteriaceae-positive. Five Salmonella-positives were recorded during the study. No indication that the sanitizer was effective in reducing Salmonella prevalence when applied as foam was observed (3/120 vs. 1/120). No Salmonella were recovered from spray-treated eggs. No statistically significant difference for Salmonella prevalence was noted, but with such a low rate of recovery it is difficult to draw a firm conclusion. However, the sanitizer applied as foam was effective at decreasing the prevalence of Enterobacteriaceae (a family of bacteria that includes Salmonella and Escherichia coli), and is present more often and in higher numbers than Salmonella. C1 [Musgrove, M. T.; Bourassa, D. V.; Cox, N. A.; Berrang, M. E.; Buhr, R. J.] USDA ARS, Richard B Russell Agr Res Ctr, Athens, GA 30605 USA. [Stephens, C. B.; Mauldin, J. M.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. RP Berrang, ME (reprint author), USDA ARS, Richard B Russell Agr Res Ctr, 950 Coll Stn Rd, Athens, GA 30605 USA. EM mark.berrang@ars.usda.gov NR 39 TC 1 Z9 1 U1 1 U2 10 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD SEP PY 2014 VL 23 IS 3 BP 516 EP 522 DI 10.3382/japr.2014-00975 PG 7 WC Agriculture, Dairy & Animal Science SC Agriculture GA AO3NN UT WOS:000341238100017 ER PT J AU Liu, SS Liu, YB AF Liu, Samuel S. Liu, Yong-Biao TI Reducing CO2 accumulation and its phytotoxicity to lettuce with absorbent in hermetic storage as a simulation of long-term fumigation SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Lactuca sativa; Postharvest storage; Quality; CO2 scrubbing; Ethylene absorbent; Phosphine fumigation ID PHOSPHINE FUMIGATION; BROWN STAIN; TEMPERATURE AB Long-term fumigation with pure phosphine at low-temperature injures lettuce and the injury is likely caused by a potential accumulation of CO2. In this study, iceberg and romaine lettuce were stored hermetically in fumigation chambers with and without absorbents for CO2 and ethylene for 3 days at 2 degrees C as a simulation of long-term fumigation to determine the effects of the absorbents on accumulations of CO2 and ethylene and postharvest quality of lettuce. In the absence of absorbents, CO2 level increased from 0.08% at the start to 3.36% at the end of the 3-day hermetic storage. No accumulation of ethylene was detected. Hermetic storage resulted in significant CO2 injuries to both iceberg and romaine lettuce and quality degradation as compared with the controls at 14 days after treatment. In the storage with absorbents, the CO2 level remained low throughout the storage and ethylene was undetectable, and the CO2 injury level was the same or lower than the control. Lettuce quality scores were either the same or better than lettuce in the control. Our findings suggest that the accumulation of CO2 alone caused injuries associated with long-term phosphine fumigation and CO2 absorbent has the potential to prevent such injuries. Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. C1 [Liu, Samuel S.; Liu, Yong-Biao] USDA ARS, Salinas, CA 93905 USA. RP Liu, YB (reprint author), USDA ARS, 1636 Alisal St, Salinas, CA 93905 USA. EM yongbiao.liu@ars.usda.gov FU U.S. Department of Agriculture, Foreign Agricultural Service FX The authors would like to thank Tanimura & Antle Co. (Salinas, California) for providing lettuce. We thank J. Wasson for technical assistance and Dr. James D. McCreight for helpful suggestions and comments. This study was supported by a Technical Assistance for Specialty Crop program of U.S. Department of Agriculture, Foreign Agricultural Service. NR 22 TC 1 Z9 1 U1 1 U2 7 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD SEP PY 2014 VL 17 IS 3 BP 435 EP 439 DI 10.1016/j.aspen.2014.04.003 PG 5 WC Entomology SC Entomology GA AN6VY UT WOS:000340737700035 ER PT J AU Shelly, T Nishimoto, J Kurashima, R AF Shelly, Todd Nishimoto, Jon Kurashima, Rick TI Distance-dependent capture probability of male Mediterranean fruit flies in trimedlure-baited traps in Hawaii SO JOURNAL OF ASIA-PACIFIC ENTOMOLOGY LA English DT Article DE Tephritidae; Detection; Trapping; Mediterranean fruit fly; Hawaii ID GINGER ROOT OIL; SPECIES DIPTERA TEPHRITIDAE; CERATITIS-CAPITATA; FLY DIPTERA; RELEASED MALES; DISPERSAL; COLEOPTERA; BUPRESTIDAE; CALIFORNIA; PATTERNS AB Many countries operate regional trapping programs for the detection of exotic tephritid fruit flies, which because of their polyphagous habits pose a serious threat to fruit and vegetable crops. Detection of the Mediterranean fruit fly (medfly), Ceratitis capitate (Wiedemann), relies primarily on trimedlure (TML), a synthetic male-specific lure, yet few studies have measured the relationship between distance from TML-baited traps and the probability of male capture, and consequently the detection sensitivity of medfly trapping programs is largely unknown. The present study measured distance-dependent capture probabilities for male C capitata in TML-baited traps using mark-release-recapture procedures. Releases were performed at distances of 25, 50, 100, and 200 m at 4 sites in Hawaii, and the resulting capture rates were used to estimate the minimum detectable population size (detection probability > 99.9%) for a trapping density of 5 TML traps per 2.59 km(2) (= 1 mi(2), the density used in California, USA). Capture rates were similar for 3 of the sites (6.5%, 3.8%, 1.1%, and 0.1% for the 4 distances, respectively) and yielded an estimated minimum detectable population of approximate to 2300 males, a value similar to that obtained in a comparable study conducted in California. For unknown reasons, capture rates were significantly lower at the remaining site (1.8%, 0.6%, 0.1%, 0.04%) and the estimated minimum detectable population was correspondingly much larger (approximate to 10,000 males). Implications of these results for medfly detection programs are discussed. Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society. C1 [Shelly, Todd; Nishimoto, Jon; Kurashima, Rick] USDA APHIS, Waimanalo, HI 96795 USA. RP Shelly, T (reprint author), USDA APHIS, 41-650 Ahiki St, Waimanalo, HI 96795 USA. EM todd.e.shelly@aphis.usda.gov NR 38 TC 2 Z9 2 U1 1 U2 15 PU KOREAN SOC APPLIED ENTOMOLOGY PI SUWON PA NATL INST AGRICULTURAL SCIENCE & TECHNOLOGY, DIVISION ENTOMOLOGY, RDA, 249 SEODUN-DONG, SUWON, 441-707, SOUTH KOREA SN 1226-8615 EI 1876-7990 J9 J ASIA-PAC ENTOMOL JI J. Asia-Pac. Entomol. PD SEP PY 2014 VL 17 IS 3 BP 525 EP 530 DI 10.1016/j.aspen.2014.05.001 PG 6 WC Entomology SC Entomology GA AN6VY UT WOS:000340737700049 ER PT J AU Yu, DH Waterland, RA Zhang, PM Schady, D Chen, MH Guan, YT Gadkari, M Shen, LL AF Yu, Da-Hai Waterland, Robert A. Zhang, Pumin Schady, Deborah Chen, Miao-Hsudh Guan, Yongtao Gadkari, Manasi Shen, Lanlan TI Targeted p16(Ink4a) epimutation causes tumorigenesis and reduces survival in mice SO JOURNAL OF CLINICAL INVESTIGATION LA English DT Article ID ABERRANT DNA METHYLATION; CPG-ISLAND; HEPATOCELLULAR-CARCINOMA; CHRONIC HEPATITIS; EARLY EVENT; CANCER; ELEMENTS; GENE; HYPERMETHYLATION; INACTIVATION AB Cancer has long been viewed as a genetic disease; however, epigenetic silencing as the result of aberrant promoter DNA methylation is frequently associated with cancer development, suggesting an epigenetic component to the disease. Nonetheless, it has remained unclear whether an epimutation (an aberrant change in epigenetic regulation) can induce tumorigenesis. Here, we exploited a functionally validated cis-acting regulatory element and devised a strategy to induce developmentally regulated genomic targeting of DNA methylation. We used this system to target DNA methylation within the p16(Ink4a) promoter in mice in vivo. Engineered p16(Ink4a) promoter hypermethylation led to transcriptional suppression in somatic tissues during aging and increased the incidence of spontaneous cancers in these mice. Further, mice carrying a germline p16(Ink4a) mutation in one allele and a somatic epimutation in the other had accelerated tumor onset and substantially shortened tumor-free survival. Taken together, these results provide direct functional evidence that p16(Ink4a) epimutation drives tumor formation and malignant progression and validate a targeted methylation approach to epigenetic engineering. C1 [Yu, Da-Hai; Waterland, Robert A.; Chen, Miao-Hsudh; Guan, Yongtao; Gadkari, Manasi; Shen, Lanlan] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Waterland, Robert A.; Guan, Yongtao] Baylor Coll Med, Dept Mol & Human Genet, Texas Childrens Hosp, Houston, TX 77030 USA. [Zhang, Pumin] Baylor Coll Med, Dept Mol Physiol & Biophys, Texas Childrens Hosp, Houston, TX 77030 USA. [Schady, Deborah] Baylor Coll Med, Dept Pathol, Texas Childrens Hosp, Houston, TX 77030 USA. RP Shen, LL (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, 1100 Bates St,Ste 8020, Houston, TX 77030 USA. EM Lanlan.Shen@bcm.edu FU NIH/NCI [P30CA125123]; Morphology core at the Texas Medical Center Digestive Disease Center (NIH/NIDDK) [P30DK56338]; Sidney Kimmel Foundation [CRIS-6250-51000-055, CRIS-6250-51000-054]; March of Dimes [1-FY-08-392]; NIDDK [1R01DK081557] FX We thank Wei Zhu, Wei Wei, and Jingming Shu for technical assistance and Adam Gillum for assistance with the figures. We acknowledge the shared resources by Baylor College of Medicine ESC and GEM cores (NIH/NCI grant P30CA125123) and by Morphology core at the Texas Medical Center Digestive Disease Center (NIH/NIDDK grant P30DK56338). This work was supported by grants from the Sidney Kimmel Foundation (to L. Shen), USDA (CRIS-6250-51000-055 to L. Shen and R.A. Water land; CRIS-6250-51000-054 to M.H. Chen), March of Dimes (1-FY-08-392 to R.A. Water land), and NIDDK (1R01DK081557 to R.A. Water land). NR 32 TC 23 Z9 23 U1 0 U2 14 PU AMER SOC CLINICAL INVESTIGATION INC PI ANN ARBOR PA 35 RESEARCH DR, STE 300, ANN ARBOR, MI 48103 USA SN 0021-9738 EI 1558-8238 J9 J CLIN INVEST JI J. Clin. Invest. PD SEP PY 2014 VL 124 IS 9 BP 3708 EP 3712 DI 10.1172/JCI76507 PG 5 WC Medicine, Research & Experimental SC Research & Experimental Medicine GA AO2PQ UT WOS:000341168100011 PM 25061879 ER PT J AU Dikmen, S Khan, FA Huson, HJ Sonstegard, TS Moss, JI Dahl, GE Hansen, PJ AF Dikmen, S. Khan, F. A. Huson, H. J. Sonstegard, T. S. Moss, J. I. Dahl, G. E. Hansen, P. J. TI The SLICK hair locus derived from Senepol cattle confers thermotolerance to intensively managed lactating Holstein cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE heat stress; SLICK locus; body temperature; milk yield ID MILK-PRODUCTION; HEAT-STRESS; DAIRY-COWS; TEMPERATURES; ENVIRONMENT AB The SLICK haplotype (http://omia.angis.org.au/OMIA001372/9913/) in cattle confers animals with a short and sleek hair coat. Originally identified in Senepol cattle, the gene has been introduced into Holsteins. The objectives of the current study were to determine (1) whether lactating Holsteins with the slick hair phenotype have superior ability for thermoregulation compared with wild-type cows or relatives not inheriting the SLICK haplotype, and (2) whether seasonal depression in milk yield would be reduced in SLICK cows. In experiment 1, diurnal variation in vaginal temperature in the summer was monitored for cows housed in a freestall barn with fans and sprinklers. Vaginal temperatures were lower in slick-haired cows than in relatives and wild-type cows. In experiment 2, acute responses to heat stress were monitored after cows were moved to a dry lot in which the only heat abatement was shade cloth. The increases in rectal temperature and respiration rate caused by heat stress during the day were lower for slick cows than for relatives or wildtype cows. Moreover, sweating rate was higher for slick cows than for cows of the other 2 types. In experiment 3, effects of season of calving (summer vs. winter) on milk yield and composition were determined. Compared with milk yield of cows calving in winter, milk yield during the first 90 d in milk was lower for cows calving in the slimmer. However, this reduction was less pronounced for slick cows than for wild-type cows. In conclusion, Holsteins with slick hair have superior thermoregulatory ability compared with non-slick animals and experience a less drastic depression in milk yield during the summer. C1 [Dikmen, S.] Uludag Univ, Dept Anim Sci, Fac Vet Med, TR-16059 Bursa, Turkey. [Khan, F. A.; Moss, J. I.; Dahl, G. E.; Hansen, P. J.] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. [Huson, H. J.; Sonstegard, T. S.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Hansen, PJ (reprint author), Univ Florida, Dept Anim Sci, Gainesville, FL 32611 USA. EM Hansen@animal.ufl.edu OI Hansen, Peter/0000-0003-3061-9333 FU USDA National Institute of Food and Agriculture (Washington, DC) [2010-85122-20623, 2013-68004-20365]; Southeast Milk Inc. Milk Check-off Program FX This research was supported by Agriculture and Food Research Initiative Competitive Grants no. 2010-85122-20623 and 2013-68004-20365 from the USDA National Institute of Food and Agriculture (Washington, DC) and a grant from Southeast Milk Inc. Milk Check-off Program. The authors acknowledge the efforts of Timothy A. Olson in establishing the SLICK gene in Holsteins, data collection by Anna C. Denicol, Sofia Ortega, and Paula Tribulo (all from Department of Animal Sciences, University of Florida, Gainesville), and the assistance of Eric Diepersloot and staff of the University of Florida Dairy Unit. NR 21 TC 10 Z9 10 U1 0 U2 13 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD SEP PY 2014 VL 97 IS 9 BP 5508 EP 5520 DI 10.3168/jds.2014-8087 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AN4TC UT WOS:000340580400023 PM 24996281 ER PT J AU Nonnecke, BJ McGill, JL Ridpath, JF Sacco, RE Lippolis, JD Reinhardt, TA AF Nonnecke, B. J. McGill, J. L. Ridpath, J. F. Sacco, R. E. Lippolis, J. D. Reinhardt, T. A. TI Acute phase response elicited by experimental bovine diarrhea virus (BVDV) infection is associated with decreased vitamin D and E status of vitamin-replete preruminant calves SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE bovine viral diarrhea virus (BVDV); preruminant calf; neonatal vitamin D status; vitamin E status and infection; acute phase response ID RESPIRATORY SYNCYTIAL VIRUS; RRR-ALPHA-TOCOPHEROL; FAT-SOLUBLE VITAMINS; SERUM-AMYLOID-A; VIRAL DIARRHEA; MILK REPLACER; GAMMA-TOCOPHEROL; IMMUNE-RESPONSES; DAIRY-COWS; T-CELLS AB Studies in young animals have shown an association between vitamin deficiencies and increased risk of infectious disease; however, there is a paucity of information regarding the effect of acute infection on the vitamin status of the vitamin-replete neonate. To characterize the effects of acute infection on vitamin D and E status of the neonate, 6 vitamin-replete preruminant Holstein bull calves were experimentally infected with bovine viral diarrhea virus (BVDV; strain BVDV2-1373). Six mock-inoculated calves served as controls. Sustained pyrexia, leukopenia, and asynchronous increases in serum haptoglobin and serum amyloid A characterized the response of calves to infection with BVDV. Infection was also associated with increased serum IFN-gamma, IL-2, and IL-6 concentrations. During the last 8 d of the 14-d postinoculation period, serum 25-hydroxyvitamin D and alpha-tocopherol concentrations in infected calves decreased by 51 and 82%, respectively. The observed inverse association between vitamin D and E status and serum amyloid A in infected calves suggests that the infection-induced acute phase response contributed to the reduced vitamin status of these animals. Additional studies are necessary to determine if the negative effect of infection on status are unique to this specific infection model or is representative of preruminant calf's response to acute infection. Studies are also needed to characterize mechanisms underlying infection-related changes in vitamin D and E status and to determine whether additional vitamin D or E supplementation during an acute infection diminishes disease severity and duration in the young animal. C1 [Nonnecke, B. J.; McGill, J. L.; Ridpath, J. F.; Sacco, R. E.; Lippolis, J. D.; Reinhardt, T. A.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. RP Nonnecke, BJ (reprint author), ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. EM nonnecke@iowatelecom.net RI Reinhardt, Timothy/A-7536-2009 OI Reinhardt, Timothy/0000-0001-5552-2509 NR 64 TC 1 Z9 1 U1 2 U2 12 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD SEP PY 2014 VL 97 IS 9 BP 5566 EP 5579 DI 10.3168/jds.2014-8293 PG 14 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AN4TC UT WOS:000340580400028 PM 25022687 ER PT J AU Arndt, C Armentano, LE Hall, MB AF Arndt, C. Armentano, L. E. Hall, M. B. TI Corn bran versus corn grain at 2 levels of forage: Intake, apparent digestibility, and production responses by lactating dairy cows SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE corn bran; corn grain; fiber source ID REDUCED-STARCH DIET; NONFORAGE FIBER; MILK-YIELD; PERFORMANCE; BLOOD; CARBOHYDRATE; FERMENTATION; REPLACEMENT; CHLORIDES; SOYHULLS AB The objective of this study was to determine the effect of substituting corn bran (CB) for dried ground corn grain (CG) in the nonforage portion of high-forage (HF) and low-forage (LF) diets. Twelve multiparous and 12 primiparous Holsteins were assigned to 4 diets using six 4 x 4 Latin squares with 3-wk periods. Forage was 64 or 38% of the total mixed ration (% of dry matter). On a dry matter basis, the HFCG diet had 20% CG, the LFCG diet had 39% CG, the HFCB diet had 19% CB, and the LFCB diet had 38% CB. Digestible organic matter intake (OMI) and milk energy yield were lower for CB compared with CG within forage level. Digestible OMI was greater (1.9 kg/d) for the LFCG compared with the HFCG treatment. When CB replaced forage (LFCB vs. HFCB), digestible OMI was not different but milk energy yield was greater with the LFCB diet. The LFCG diet supported the greatest milk, milk protein, and milk energy yield. Decreased concentration of milk protein and increased concentration of milk urea nitrogen when feeding CB compared with CG suggests that lack of fermentable energy in the CB diets may have limited rumen microbial protein synthesis. Total substitution of CG with CB in the nonforage portion did not support maximum milk production, even when forage was reduced at the same time (HFCG vs. LFCB). Predicted neutral detergent fiber (NDF) digestibility at 1 times maintenance, based on chemical analysis of the individual feeds, was 22 percentage units greater for CB than for the forage mix (68.9 vs. 46.9%). In vitro NDF digestibility (30 h) was 19.4 percentage units greater for CB than for the forage mix (68.9 vs. 49.5%). However, in vivo NDF digestibility of the diet when CB replaced forage (HFCB vs. LFCB) was similar (44.1 vs. 44.5%). Similarly, predicted total digestible nutrients at the production level of intake, based on chemical analysis, were greater for the CB treatments and lower for the CO treatments than those observed in vivo. C1 [Arndt, C.; Armentano, L. E.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Hall, M. B.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Armentano, LE (reprint author), Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. EM learment@wisc.edu FU Wisconsin Corn Promotion Board (Palmyra) FX The authors thank the Wisconsin Corn Promotion Board (Palmyra) for their financial support and Renew Energy (Jefferson, WI) for donation of the corn bran. Appreciation is expressed to Jim Meronek and his staff at the US Dairy Forage Research Center (Prairie du Sac, WI) for animal care, marker bolusing and dosing, feeding, milking, and sample collection; Peter Crump of University of Wisconsin-Madison College of Agricultural and Life Sciences for assisting with statistical analyses; and Barry Bradford at Kansas State University (Manhattan) and Paul Kononoff at the University of Nebraska (Lincoln) for the corn gluten feed samples. NR 30 TC 2 Z9 2 U1 1 U2 11 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD SEP PY 2014 VL 97 IS 9 BP 5676 EP 5687 DI 10.3168/jds.2013-7815 PG 12 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AN4TC UT WOS:000340580400039 PM 24997669 ER PT J AU Pinto, SM Pearson, DE Maron, JL AF Pinto, Sarah M. Pearson, Dean E. Maron, John L. TI Seed dispersal is more limiting to native grassland diversity than competition or seed predation SO JOURNAL OF ECOLOGY LA English DT Article DE determinants of plant community diversity and structure; dispersal limitation; dominance; relative abundance; seedling establishment; species richness ID DOMINANT SPECIES IDENTITY; PLANT-POPULATIONS; SPATIAL-PATTERNS; PRODUCTIVITY GRADIENT; PERENNIAL GRASSLAND; COMMUNITY ECOLOGY; CHIHUAHUAN DESERT; BIOTIC RESISTANCE; RECRUITMENT; RICHNESS AB 1. Competition has historically been viewed as the predominant process affecting plant community structure. In particular, it is often assumed that the dominant resident species is the superior competitor and therefore has large impacts on plant community diversity. This assumption, however, is seldom tested. As well, there are a variety of other processes such as dispersal limitation and seed predation that can influence community structure, although the relative importance of these processes in relation to competition from resident species is still unclear. 2. We examined how interspecific competition, dispersal limitation and post-dispersal seed predation by mice individually and interactively influenced the richness and diversity of local plant assemblages in grasslands of western Montana. 3. We added seeds of 20 mostly locally uncommon native species to subplots in and out of larger rodent exclosure plots. Using a 'species-blind' approach, we manipulated competition from resident plants in these subplots by removing the same amount of cover of one dominant species or several common species. 4. The species richness and diversity of local assemblages was higher in subplots with seed addition than without. Across all levels of the other treatments, average richness for subplots without competitor removal was lower than for competitor removal treatments. However, the removal of one locally dominant species had similar effects to removal of several common species. In contrast, preventing seed predation by mice did not have significant effects on richness. There were no significant interactions between the treatments. 5. Synthesis. Our results reveal a hierarchy of filters that determine local community structure. Many regionally rare species were dispersal limited and established after the seed addition, regardless of release from competition or the presence of seed predators. Subsequently, we found competitive equivalence between dominant and common species. C1 [Pinto, Sarah M.; Pearson, Dean E.; Maron, John L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Pearson, Dean E.] US Forest Serv, USDA, Rocky Mt Res Stn, Forestry Sci Lab, Missoula, MT 59801 USA. RP Pinto, SM (reprint author), Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. EM sarahmariannepinto@gmail.com FU USDA Cooperative State Research; Education and Extension Service [2005-35101-16040]; NSF [DEB-0614406]; U.S. Bureau of Land Management FX We thank Ray Callaway, Winsor Lowe, Lisa Eby and Yvette Ortega for their extensive guidance and feedback. We thank Teal Potter, Loren Wombold, Peter Lesica and Rebecca Fletcher for their field assistance. We thank the Montana Department of Fish Wildlife and Parks, the U. S. Fish and Wildlife Service, the University of Montana, and the E Bar L Ranch and Gloria and Doug Roarke for allowing us to work on their lands. JLM and DEP received funding for this work through grants from the USDA Cooperative State Research, Education and Extension Service (2005-35101-16040), NSF (DEB-0614406) and the U.S. Bureau of Land Management. NR 65 TC 5 Z9 5 U1 5 U2 81 PU WILEY PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-0477 EI 1365-2745 J9 J ECOL JI J. Ecol. PD SEP PY 2014 VL 102 IS 5 BP 1258 EP 1265 DI 10.1111/1365-2745.12282 PG 8 WC Plant Sciences; Ecology SC Plant Sciences; Environmental Sciences & Ecology GA AN6CM UT WOS:000340681300016 ER PT J AU Freeland, RS Allred, BJ Sorochan, JC AF Freeland, Robert S. Allred, Barry J. Sorochan, John C. TI Profiling USGA Putting Greens Using GPR-An As-built Surveying Method SO JOURNAL OF ENVIRONMENTAL AND ENGINEERING GEOPHYSICS LA English DT Article ID GROUND-PENETRATING RADAR AB Putting greens that are constructed to United State Golf Association (USGA) standards must adhere to strict installation specifications to maintain proper subsurface drainage. Because the infrastructure is buried, it can be difficult to ascertain if these structures were properly installed. Insufficient drainage affects agronomics, compromising the playability of the putting surface. Repairs are expensive and laborious. This study introduces a nonintrusive survey protocol that combines ground-penetrating radar (GPR) and real-time kinematic (RTK) positioning. A case study was used to examine a putting green designed using the USGA specifications. The protocol created "as-built" subsurface maps. The protocol non-intrusively identified the tile slope and spacing. It also determined the depth and thickness of the root-zone mixture and gravel. The green adhered to the tile spacing specification, but failed to meet specifications as to tile slope and root-zone mixture depth. These findings suggest that this protocol provides a relatively inexpensive method to determine adherence to USGA greens standards. Generated maps highlighted conformity (or lack thereof) to the design specifications. C1 [Freeland, Robert S.] Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA. [Allred, Barry J.] ARS, USDA, Soil Drainage Res Unit, Columbus, OH 43210 USA. [Sorochan, John C.] Univ Tennessee, Dept Plant Sci, Knoxville, TN 37996 USA. RP Freeland, RS (reprint author), Univ Tennessee, Dept Biosyst Engn & Soil Sci, 2506 EJ Chapman Dr, Knoxville, TN 37996 USA. EM rfreelan@utk.edu; allred.13@osu.edu; sorochan@utk.edu NR 12 TC 2 Z9 2 U1 2 U2 4 PU ENVIRONMENTAL ENGINEERING GEOPHYSICAL SOC PI DENVER PA 1720 SOUTH BELLAIRE, STE 110, DENVER, CO 80222-433 USA SN 1083-1363 J9 J ENVIRON ENG GEOPH JI J. Environ. Eng. Geophys. PD SEP PY 2014 VL 19 IS 3 BP 167 EP 174 DI 10.2113/JEEG19.3.167 PG 8 WC Geochemistry & Geophysics; Engineering, Geological SC Geochemistry & Geophysics; Engineering GA AO2QF UT WOS:000341169600004 ER PT J AU Haq, I Vreysen, MJB Caceres, C Shelly, TE Hendrichs, J AF Haq, Ihsan Vreysen, Marc J. B. Caceres, Carlos Shelly, Todd E. Hendrichs, Jorge TI Methyl eugenol aromatherapy enhances the mating competitiveness of male Bactrocera carambolae Drew & Hancock (Diptera: Tephritidae) SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Carambola fruit fly; Mating success; Methyl eugenol treatment; Sterile insect technique ID ORIENTAL FRUIT-FLY; SEX-PHEROMONE COMPONENTS; STERILE INSECT TECHNIQUE; MALE-ANNIHILATION METHOD; DORSALIS DIPTERA; DACUS-DORSALIS; RELEASE; FIELD; ERADICATION; CONSUMPTION AB Males of Bactrocera carambolae Drew & Hancock (Diptera: Tephritidae) are strongly attracted to methyl eugenol (ME) (1,2-dimethoxy-4-(2-propenyl)benzene), a natural compound occurring in variety of plant species. ME-feeding is known to enhance male B. carambolae mating competitiveness 3 days after feeding. Enhanced male mating competitiveness due to ME-feeding can increase the effectiveness of sterile insect technique (SIT) manifolds. However, the common methods for emergence and holding fruit flies prior to field releases do not allow the inclusion of any ME feeding treatment after fly emergence. Therefore this study was planned to assess the effects of ME-aromatherapy in comparison with ME feeding on male B. carambolae mating competitiveness as aromatherapy is pragmatic for fruit flies emergence and holding facilities. Effects of ME application by feeding or by aromatherapy for enhanced mating competitiveness were evaluated 3d after treatments in field cages. ME feeding and ME aromatherapy enhanced male mating competitiveness as compared to untreated males. Males treated with ME either by feeding or by aromatherapy showed similar mating success but mating success was significantly higher than that of untreated males. The results are discussed in the context of application of ME by aromatherapy as a pragmatic approach in a mass-rearing facility and its implications for effectiveness of SIT. (C) 2014 The Authors. Published by Elsevier Ltd. C1 [Haq, Ihsan; Vreysen, Marc J. B.; Caceres, Carlos] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Seibersdorf, Austria. [Haq, Ihsan] Natl Agr Res Ctr, Islamabad, Pakistan. [Shelly, Todd E.] USDA APHIS, Waimanalo, HI 96795 USA. [Hendrichs, Jorge] Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Sect, Vienna, Austria. RP Haq, I (reprint author), Joint FAO IAEA Div Nucl Tech Food & Agr, Insect Pest Control Lab, Seibersdorf, Austria. EM imihsan@yahoo.com OI , Ihsan/0000-0001-5879-7698 NR 59 TC 4 Z9 4 U1 6 U2 28 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD SEP PY 2014 VL 68 BP 1 EP 6 DI 10.1016/j.jinsphys.2014.06.014 PG 6 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA AO0JJ UT WOS:000340994600001 PM 24995839 ER PT J AU Lin, LG Nuotio-Antar, AM Ma, XJ Liu, F Fiorotto, ML Sun, YX AF Lin, Ligen Nuotio-Antar, Alli M. Ma, Xiaojun Liu, Feng Fiorotto, Marta L. Sun, Yuxiang TI Ghrelin Receptor Regulates Appetite and Satiety during Aging in Mice by Regulating Meal Frequency and Portion Size but Not Total Food Intake SO JOURNAL OF NUTRITION LA English DT Article ID HORMONE SECRETAGOGUE RECEPTOR; DIET-INDUCED OBESITY; NPY/AGRP NEURONS; BODY-WEIGHT; GROWTH; RESTRICTION; METABOLISM; ADIPOSITY; AGE; CHOLECYSTOKININ AB Aging is often associated with overweight and obesity. There exists a long-standing debate about whether meal pattern also contributes to the development of obesity. The orexigenic hormone ghrelin regulates appetite and satiety by activating its receptor, growth hormone secretagogue receptor (GHS-R). In mice, circulating ghrelin concentrations and brain GHS-R expression were shown to increase with aging. To assess whether GHS-R regulates feeding pattern during aging, we studied meal patterns for the following cohorts of male mice fed a normal unpurified diet 1) 3-4 mo, young wild-type (WT) mice; 2) 3-4 mo, young Ghsr-null (Ghsr(-/-)) mice; 3) 12-14 mo, middle-aged WT (WT-M) mice; 4) 12-14 mo, middle-aged Ghsr(-/-) (Ghsr(-/-)-M) mice; 5) 24-26 mo, old WT (WT-O) mice; and 6) 24-26 mo, old Ghsr(-/-) (Ghsr(-/-)-O) mice. Although the total daily food intake of Ghsr(-/-) mice was similar to that of WT controls, Ghsr(-/-)-M and Ghsr(-/-)-O mice had 9% (P = 0.07) and 16% (P < 0.05) less body weight compared with WT-M and WT-O mice, respectively, primarily due to reduced fat mass (P < 0.05, WT-M vs. Ghsr(-/-)-M and WT-O vs. Ghsr(-/-)-O). Intriguingly, Ghsr(-/-)-M mice ate larger meals (on average, Ghsr(-/-)-M mice ate 0.117 g/meal and WT-M mice ate 0.080 g/meal; P < 0.01) and took a longer time to eat (Ghsr(-/-)-M, 196.0 s and WT-M, 128.9s; P < 0.01), but ate less frequently (Ghsr(-/-)-M, 31.0 times/d and WT-M, 42.3 times/d; P < 0.05) than WT-M controls. In addition, we found that expression of hypothalamic orexigenic peptides, neuropeptide Y (NPY) and agouti-related peptide (AgRP), was relatively lower in aged WT mice (P = 0.09 for NPY and P = 0.06 for AgRP), but anorexic peptide pro-opiomelanocortin (POMC) expression remained unchanged between the WT age groups. Interestingly, old Ghsr(-/-) mice had greater hypothalamic NPY expression (102% higher; P < 0.05) and AgRP expression (P = 0.07) but significantly lower POMC expression (P < 0.05) when compared with age-matched WT-O controls. Thus, our results indicate that GHS-R plays an important role in the regulation of meal pattern and that GHS-R ablation may modulate feeding behavior through the regulation of hypothalamic neuropeptides. Our results collectively suggest that ghrelin receptor antagonism may have a beneficial effect on metabolism during aging. C1 [Lin, Ligen; Nuotio-Antar, Alli M.; Ma, Xiaojun; Fiorotto, Marta L.; Sun, Yuxiang] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. [Lin, Ligen] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macao, Peoples R China. [Ma, Xiaojun] Zhengzhou Univ, Affiliated Hosp 1, Dept Internal Med, Div Endocrinol, Zhengzhou 450052, Peoples R China. [Liu, Feng] Univ Texas Hlth Sci Ctr San Antonio, Dept Pharmacol, San Antonio, TX 78229 USA. [Liu, Feng] Cent S Univ, Xiangya Hosp 2, Metab Syndrome Res Ctr, Changsha, Hunan, Peoples R China. RP Sun, YX (reprint author), Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. EM yuxiangs@bcm.edu FU USDA/Current Research Information System [ARS 6250-51000-055]; AHA [12IRG9230004, 14GRNT18990019, 10POST3850002]; H/Diabetes and Endocrinology Research Center of Baylor College of Medicine [P30DK079638]; Macao Science and Technology Development Fund [042/2013/A2]; University of Macau [MRG015/LLG/2014/ICMS] FX This study was supported by USDA/Current Research Information System grant ARS 6250-51000-055 (to Y.S.), AHA innovative grant 12IRG9230004 (to Y.S.) and grant-in-aid 14GRNT18990019 (to Y.S), NIH/Diabetes and Endocrinology Research Center of Baylor College of Medicine grant P30DK079638, AHA postdoctoral fellowship award 10POST3850002 (to A.M.N.-A.), Macao Science and Technology Development Fund grant 042/2013/A2 (to L.L.), and the University of Macau grant MRG015/LLG/2014/ICMS (to L.L.). NR 37 TC 6 Z9 6 U1 0 U2 13 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD SEP PY 2014 VL 144 IS 9 BP 1349 EP 1355 DI 10.3945/jn.114.191171 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AN6VK UT WOS:000340736300001 PM 24991043 ER PT J AU Nachman, RJ Kaczmarek, K Jiang, H Wei, ZJ Zabrocki, J Park, Y AF Nachman, R. J. Kaczmarek, K. Jiang, H. Wei, Zhaojun Zabrocki, J. Park, Y. TI A SELECTIVE CAP2b NEUROPEPTIDE ANTAGONIST FOR AN EXPRESSED RECEPTOR FROM THE RED FLOUR BEETLE, TRIBOLIUM CASTANEUM SO JOURNAL OF PEPTIDE SCIENCE LA English DT Meeting Abstract DE pyrokinin; diuresis; pseudopeptide; pest management; neuroendocrinology C1 [Nachman, R. J.; Kaczmarek, K.; Zabrocki, J.] USDA, Insect Control & Cotton Dis Res Unit, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. [Kaczmarek, K.; Zabrocki, J.] Tech Univ Lodz, Inst Organ Chem, PL-90924 Lodz, Poland. [Jiang, H.; Wei, Zhaojun; Park, Y.] Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1075-2617 EI 1099-1387 J9 J PEPT SCI JI J. Pept. Sci. PD SEP PY 2014 VL 20 SU 1 MA P082 BP S162 EP S163 PG 2 WC Biochemistry & Molecular Biology; Chemistry, Analytical SC Biochemistry & Molecular Biology; Chemistry GA AN8TD UT WOS:000340876400227 ER PT J AU Ganey, JL Ward, JP Jenness, JS Block, WM Hedwall, S Jonnes, RS Apprill, DL Rawlinson, TA Kyle, SC Spangle, SL AF Ganey, Joseph L. Ward, James P., Jr. Jenness, Jeffrey S. Block, William M. Hedwall, Shaula Jonnes, Ryan S. Apprill, Darrell L. Rawlinson, Todd A. Kyle, Sean C. Spangle, Steven L. TI USE OF PROTECTED ACTIVITY CENTERS BY MEXICAN SPOTTED OWLS IN THE SACRAMENTO MOUNTAINS, NEW MEXICO SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Mexican Spotted Owl; Strix occidentalis lucida; conservation; nest sites; Protected Activity Center; recolonization; roost ID AREAS AB A Recovery Plan developed for the threatened Mexican Spotted Owl (Strix occidentalis lucida) recommended designating Protected Activity Centers (PACs) with a minimum size of 243 ha to conserve core use areas of territorial owls. The plan assumed that areas of this size would protect "... the nest site, several roost sites, and the most proximal and highly-used foraging areas." The PAC concept remains an important component of the recovery strategy nineteen years later, although use of designated PACs by territorial owls has never been evaluated. We assessed use of PACs for nesting and roosting by Mexican Spotted Owls in the Sacramento Mountains, New Mexico, using location data obtained during a study of owl demography from 2004-2011. High proportions of both nest and roost locations were located within the PAC boundary for most, but not all, PACs. Many locations outside of PAC boundaries were adjacent to those boundaries, but some occurred >1 km from PAC boundaries. Proportions of roost locations within the PAC also were high for most, but not all, individual owls of both sexes, and in all years of the study. Proportions of locations within PACs remained relatively high for periods of up to 24 yr following PAC establishment, suggesting that owls continued to use these areas over relatively long periods. A number of vacant PACs were recolonized by owls during the study, and these owls also used PAC areas at high levels in most, but not all, cases. It would be desirable to assess PAC use over longer time periods, in other geographic areas, and to incorporate foraging use in such evaluations. In the meantime, however, our C1 [Ganey, Joseph L.; Block, William M.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. [Ward, James P., Jr.] US Fish & Wildlife Serv, Natl Wildlife Refuge Syst, Inventory & Monitoring Branch, Ft Collins, CO 80525 USA. [Jenness, Jeffrey S.] Jenness Enterprises, Flagstaff, AZ 86004 USA. [Hedwall, Shaula] US Fish & Wildlife Serv, Arizona Ecol Serv Off, Flagstaff, AZ 86001 USA. [Jonnes, Ryan S.; Apprill, Darrell L.] US Forest Serv, Lincoln Natl Forest, Cloudcroft, NM 88317 USA. [Rawlinson, Todd A.] US Forest Serv, Lincoln Natl Forest, Ruidoso, NM 88345 USA. [Kyle, Sean C.] Texas Parks & Wildlife Dept, Lubbock, TX 79415 USA. [Spangle, Steven L.] US Fish & Wildlife Serv, Arizona Ecol Serv Off, Phoenix, AZ 85021 USA. RP Ganey, JL (reprint author), US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. EM jganey@fs.fed.us OI Jenness, Jeff/0000-0002-9448-1444 FU U.S. Forest Service, Southwestern Region (USFS, SW Region); Lincoln National Forest; Rocky Mountain Research Station, USFS FX We thank the many dedicated field personnel who located owls and nests within the Sacramento Mountains, including T. Bartnick, A. Behney, T. Borneman, C. Brayton, N. Brown, J. Cannon, M. Chappell, C. Cobb, M. Collado, J. Cooper, M. Crabb, R. Crandall, C. Domschke, C. Edge, T. Felkey, L. Gedacht, C. Glenney, J. Gorey, J. Goyette, J. Groce, S. Halsey, D. Harrington, T. Heard, M. Hillman, T. Holland, J. Hyre, M. Ihnken, S. Isham, J. Jerrett, R. Johnson, J. Justus, M. Kern, D. Kite, R. Landry, R. Lavier, A. Mahoney, R. McLain, P. Mercer, S. Miller, F. Monreal, C. Mosby, L. Navarrete, M. Neely, C. Okraska, H. Oswald, A. Parrish, M. Peterson, R. Peterson, E. Pollom, D. Skalos, C. Schmidt, N. Smith, M. Riley, B. Rubeck, A. Salonikios, R. Seeley, G. Sorrentino, C. Starkweather, J. Taylor, R. Trujillo, N. Unsworth, A. VandeVoort, N. von Hedeman, K. Wagner, A. Walters, K. Weber, and J. Whiteman. We also thank personnel on the Sacramento Ranger District, Lincoln National Forest (especially M. Mauter, J. Montoya, R. Guaderrama, D. Salas, and J. Williams), for operational support during the Sacramento Mountains demography study, as well as the numerous landowners who graciously allowed us to access call points located on private land. Major funding was provided by the U.S. Forest Service, Southwestern Region (USFS, SW Region), with additional funding from the Lincoln National Forest and Rocky Mountain Research Station, USFS. We thank D. DeLorenzo (USFS, SW Region) for his support and assistance with securing funding throughout the study. L.S. Baggett provided advice on statistical methods, and J. Keane and two anonymous reviewers provided helpful comments on an earlier draft of this paper. NR 18 TC 1 Z9 1 U1 1 U2 8 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD SEP PY 2014 VL 48 IS 3 BP 210 EP 218 DI 10.3356/JRR-13-18.1 PG 9 WC Ornithology SC Zoology GA AO1SZ UT WOS:000341096200002 ER PT J AU Humphrey, JS Avery, ML AF Humphrey, John S. Avery, Michael L. TI IMPROVED SATELLITE TRANSMITTER HARNESS ATTACHMENT TECHNIQUE SO JOURNAL OF RAPTOR RESEARCH LA English DT Article DE Black Vulture; Coragyps atratus; attachment method; backpack harness; satellite transmitter ID ALBATROSSES; TRACKING C1 [Humphrey, John S.; Avery, Michael L.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Florida Field Stn, Gainesville, FL 32641 USA. RP Humphrey, JS (reprint author), Wildlife Serv, USDA, Natl Wildlife Res Ctr, Florida Field Stn, 2820 East Univ Ave, Gainesville, FL 32641 USA. EM michael.l.avery@aphis.usda.gov FU U.S.D.A. Wildlife Services [N62467-06-RP-00202]; Southern Division, Naval Facilities Engineering Command [N62467-06-RP-00202] FX For animal care services, we thank K.L. Keacher and W.E. Bruce. We appreciate the field assistance provided by T.S. Daughtery and J. Wallace. Funding for field research was through contract N62467-06-RP-00202 between the U.S.D.A. Wildlife Services and the Southern Division, Naval Facilities Engineering Command. NR 12 TC 1 Z9 1 U1 0 U2 8 PU RAPTOR RESEARCH FOUNDATION INC PI HASTINGS PA 14377 117TH STREET SOUTH, HASTINGS, MN 55033 USA SN 0892-1016 EI 2162-4569 J9 J RAPTOR RES JI J. Raptor Res. PD SEP PY 2014 VL 48 IS 3 BP 289 EP 291 DI 10.3356/JRR-13-26.1 PG 3 WC Ornithology SC Zoology GA AO1SZ UT WOS:000341096200011 ER PT J AU Fragoso, CAV Hsu, FC Brinkley, T Church, T Liu, CK Manini, T Newman, AB Stafford, RS McDermott, MM Gill, TM AF Fragoso, Carlos A. Vaz Hsu, Fang-Chi Brinkley, Tina Church, Timothy Liu, Christine K. Manini, Todd Newman, Anne B. Stafford, Randall S. McDermott, Mary M. Gill, Thomas M. CA LIFE Study Grp TI Combined Reduced Forced Expiratory Volume in 1 Second (FEV1) and Peripheral Artery Disease in Sedentary Elders With Functional Limitations SO JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION LA English DT Article DE FEV1; peripheral artery disease; mobility; sedentary ID ANKLE-BRACHIAL INDEX; OBSTRUCTIVE PULMONARY-DISEASE; LEG SYMPTOMS; UNITED-STATES; OLDER-ADULTS; RISK-FACTORS; SPIROMETRY; LUNG; PREDICTOR; DECLINE AB Objectives: Because they are potentially modifiable and may coexist, we evaluated the combined occurrence of a reduced forced expiratory volume in 1 second (FEV1) and peripheral artery disease (PAD), including its association with exertional symptoms, physical inactivity, and impaired mobility, in sedentary elders with functional limitations. Design: Cross sectional. Setting: Lifestyle Interventions and Independence in Elder (LIFE) Study. Participants: A total of 1307 sedentary community-dwelling persons, mean age 78.9, with functional limitations (Short Physical Performance Battery [SPPB] < 10). Measurements: A reduced FEV1 was defined by a z-score less than -1.64 (< lower limit of normal), whereas PAD was defined by an ankle-brachial index less than 1.00. Exertional dyspnea was defined as moderate to severe (modified Borg index) immediately after a 400-meter walk test (400MWT). Exertional leg symptoms were established by the San Diego Claudication Questionnaire. Physical inactivity was evaluated by percent of accelerometry wear-time with activity less than 100 counts per minute (top quartile established high sedentary time). Mobility was evaluated by the 400MWT (gait speed < 0.8 m/s defined as slow) and SPPB (<= 7 defined moderate-to-severe mobility impairment). Results: A combined reduced FEV1 and PAD was established in 6.0% (78/1307) of participants. However, among those who had a reduced FEV1, 34.2% (78/228) also had PAD, whereas 20.8% (78/375) of those who had PAD also had a reduced FEV1. The 2 combined conditions were associated with exertional dyspnea (adjusted odds ratio [adjOR] 2.59 [1.20-5.60]) and slow gait speed (adjOR 3.15 [1.72-5.75]) but not with exertional leg symptoms, high sedentary time, and moderate-to-severe mobility impairment. Conclusions: In sedentary community-dwelling elders with functional limitations, a reduced FEV1 and PAD frequently coexisted and, in combination, were strongly associated with exertional dyspnea and slow gait speed (a frailty indicator that increases the risk of deleterious outcomes). Published by Elsevier Inc. on behalf of AMDA - The Society for Post-Acute and Long-Term Care Medicine. C1 [Fragoso, Carlos A. Vaz] VA Connecticut, Clin Epidemiol Res Ctr, West Haven, CT USA. [Fragoso, Carlos A. Vaz; Gill, Thomas M.] Yale Univ, Sch Med, Dept Med, New Haven, CT 06510 USA. [Hsu, Fang-Chi] Wake Forest Sch Med, Dept Biostat Sci, Winston Salem, NC USA. [Brinkley, Tina] Wake Forest Sch Med, Sticht Ctr Aging, Winston Salem, NC USA. [Church, Timothy] Louisiana State Univ, Pennington Biomed Res Ctr, Baton Rouge, LA 70808 USA. [Church, Timothy] Klein Buendel Inc, Golden, CO USA. [Liu, Christine K.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Liu, Christine K.] Boston Univ, Sch Med, Dept Med, Sect Geriatr, Boston, MA 02118 USA. [Manini, Todd] Univ Florida, Dept Aging & Geriatr Res, Gainesville, FL USA. [Newman, Anne B.] Univ Pittsburgh, Dept Epidemiol & Med, Pittsburgh, PA USA. [Stafford, Randall S.] Stanford Sch Med, Stanford Prevent Res Ctr, Program Prevent Outcomes & Practices, Stanford, CA USA. [McDermott, Mary M.] Northwestern Univ, Feinberg Sch Med, Chicago, IL 60611 USA. RP Fragoso, CAV (reprint author), VA Connecticut Healthcare Syst, West Haven, CT 06516 USA. EM carlos.fragoso@yale.edu RI Buford, Thomas /B-1111-2011; Newman, Anne B./C-6408-2013 OI Buford, Thomas /0000-0002-9541-4358; Marsiske, Michael/0000-0001-5973-2116; Newman, Anne B./0000-0002-0106-1150 FU National Institutes of Health (NIH)/National Institute on Aging Cooperative Agreement [UO1 AG22376]; Intramural Research Program, National Institute on Aging, National Institutes of Health; Yale Claude D. Pepper Older Americans Independence Center [P30AG21342]; National Institute on Aging [K07AG043587] FX This study was funded by a National Institutes of Health (NIH)/National Institute on Aging Cooperative Agreement #UO1 AG22376 and a supplement from the National Heart, Lung, and Blood Institute 3U01AG022376-05A2S, and sponsored in part by the Intramural Research Program, National Institute on Aging, National Institutes of Health. Clinicaltrials.gov identifier NCT01072500.; Dr Vaz Fragoso and Dr Gill are supported by the Yale Claude D. Pepper Older Americans Independence Center (P30AG21342). Dr Vaz Fragoso is the recipient of a VA Merit Award and Dr Gill is the recipient of an Academic Leadership Award (K07AG043587) from the National Institute on Aging. NR 29 TC 3 Z9 3 U1 2 U2 5 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1525-8610 EI 1538-9375 J9 J AM MED DIR ASSOC JI J. Am. Med. Dir. Assoc. PD SEP PY 2014 VL 15 IS 9 BP 665 EP 670 DI 10.1016/j.jamda.2014.05.008 PG 6 WC Geriatrics & Gerontology SC Geriatrics & Gerontology GA AO2PM UT WOS:000341167700011 ER PT J AU He, ZQ Uchimiya, M Cao, HP AF He, Zhongqi Uchimiya, Minori Cao, Heping TI Intrinsic Fluorescence Excitation-Emission Matrix Spectral Features of Cottonseed Protein Fractions and the Effects of Denaturants SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Cottonseed; Denaturation; Excitation-emission matrix; Fluorescence; Protein isolate ID SODIUM DODECYL-SULFATE; ADHESIVE PROPERTIES; GUANIDINE-HYDROCHLORIDE; UREA AB To better understand the functional and physicochemical properties of cottonseed protein, we investigated the intrinsic fluorescence excitation-emission matrix (EEM) spectral features of cottonseed protein isolate (CSPI) and sequentially extracted water (CSPw) and alkali (CSPa) protein fractions, and the effects of denaturants urea, guanidine hydrochloride, and sodium dodecyl sulfate. The EEM showed two contour peaks at the excitation wavelengths of 226 nm (Peak 1) and 277 nm (Peak 2). Addition of denaturants gradually shifted the emission maxima of both peaks from 335 nm to around 353 nm for CSPI and CSPa. The emission maximum (353 nm) of CSPw was unchanged by denaturation. These observations indicated that the tryptophan residues (fluorescence source) in the native CSPI and CSPa were protected within the micro hydrophobic environment, and gradually become water accessible with progressing denaturation. On the other hand, the tryptophan residues in native CSPw were already in contact with water. However, the fluorescence intensity of Peak 1 of all three protein samples decreased with increasing denaturant concentrations, suggesting similarity in some conformational changes in the three samples. Further exploration of the fluorescence mechanism of Peak 1 is needed to understand such similar conformational changes. C1 [He, Zhongqi; Uchimiya, Minori; Cao, Heping] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP He, ZQ (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM zhongqi.he@ars.usda.gov OI He, Zhongqi/0000-0003-3507-5013 NR 22 TC 5 Z9 5 U1 0 U2 4 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD SEP PY 2014 VL 91 IS 9 BP 1489 EP 1497 DI 10.1007/s11746-014-2495-1 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AO1PG UT WOS:000341084900002 ER PT J AU McKeon, TA Auld, D Brandon, DL Leviatov, S He, XH AF McKeon, Thomas A. Auld, Dick Brandon, David L. Leviatov, Shai He, Xiaohua TI Toxin Content of Commercial Castor Cultivars SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Oilseed; Energy crop; Ricin; Ricinus communis; Ricin detection; Castor oil; Ricinoleic acid ID RICIN AB The castor plant Ricinus communis L. is the source of castor oil which has numerous uses and is a key chemical feedstock for an array of products from polymers to cosmetics. Although castor was once widely grown throughout the world, the presence of the toxic protein ricin has deterred the re-introduction of this useful crop. In order to develop a low-ricin or no-ricin castor crop, a robust, accurate method for screening castor seed for ricin content is essential. In this study, we validated a sandwich enzyme-linked immunosorbent assay for the detection of ricin and the homologous Ricinus communis agglutinin (RCA) in castor beans and screened a collection of castor lines including commercial varieties using this assay. Although the average content derived by extracting several seeds provided a fair representation of the ricin content for a given selection, there was enough seed-to-seed variation to justify determining the ricin/RCA content of individual seeds. Ricin levels observed ranged from 1.16 to 6.25 % by weight. C1 [McKeon, Thomas A.; Brandon, David L.; He, Xiaohua] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Auld, Dick] Texas Tech Univ, Dept Soil & Plant Sci, Lubbock, TX 79409 USA. [Leviatov, Shai] EvoFuel Ltd, IL-76121 Rehovot, Israel. RP He, XH (reprint author), 800 Buchanan St, Albany, CA 94710 USA. EM xiaohua.he@ars.usda.gov NR 16 TC 5 Z9 5 U1 4 U2 16 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD SEP PY 2014 VL 91 IS 9 BP 1515 EP 1519 DI 10.1007/s11746-014-2505-3 PG 5 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AO1PG UT WOS:000341084900005 ER PT J AU Hojilla-Evangelista, MP AF Hojilla-Evangelista, Mila P. TI Improved Solubility and Emulsification of Wet-Milled Corn Germ Protein Recovered by Ultrafiltration-Diafiltration SO JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY LA English DT Article DE Corn; Corn germ; Protein extraction; Protein functionality; Ultrafiltration-diafiltration ID FUNCTIONAL-PROPERTIES; ISOELECTRIC PRECIPITATION; WATER-RETENTION; SOY PROTEINS; FLOUR; EXTRACTION AB This study evaluated Ultrafiltration-Diafiltration (UFDF) as a means to improve the extractability of wet-milled corn germ protein and determined its effects on the functional properties of the recovered protein product. Wet germ and dried germ proteins were extracted by using 0.1 M NaCl at 50 A degrees C. Major steps in the method were stirring, centrifugation, UFDF and freeze-drying. For dried germ, the UFDF method showed marginal increase in protein extraction efficiency, while that of wet germ remained similar to what we observed for the baseline method (saline extraction with dialysis instead of UFDF). UFDF protein extracts from both germ samples were significantly more soluble than extracts recovered by the baseline method and showed atypical solubility profiles; i.e., the amount of soluble protein was essentially unchanged under acidic, neutral, and alkaline pH. UFDF-dried germ protein was markedly more soluble than UFDF-wet germ protein (80 vs 50 % soluble protein, respectively) between pH 2 and pH 10. Both of these UFDF-germ proteins also had emulsifying capacities and water-holding capacities that were superior to those of proteins extracted by the baseline method, but this favorable effect by UFDF was not observed for emulsion stability, foaming properties, and heat stability. C1 USDA ARS, Plant Polymer Res Unit, NCAUR, Peoria, IL 61604 USA. RP Hojilla-Evangelista, MP (reprint author), USDA ARS, Plant Polymer Res Unit, NCAUR, 1815 N Univ St, Peoria, IL 61604 USA. EM Mila.HojillaEvangelista@ars.usda.gov NR 28 TC 1 Z9 1 U1 1 U2 29 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0003-021X EI 1558-9331 J9 J AM OIL CHEM SOC JI J. Am. Oil Chem. Soc. PD SEP PY 2014 VL 91 IS 9 BP 1623 EP 1631 DI 10.1007/s11746-014-2503-5 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AO1PG UT WOS:000341084900016 ER PT J AU Nelson, MI Wentworth, DE Culhane, MR Vincent, AL Viboud, C LaPointe, MP Lin, XD Holmes, EC Detmer, SE AF Nelson, Martha I. Wentworth, David E. Culhane, Marie R. Vincent, Amy L. Viboud, Cecile LaPointe, Matthew P. Lin, Xudong Holmes, Edward C. Detmer, Susan E. TI Introductions and Evolution of Human-Origin Seasonal Influenza A Viruses in Multinational Swine Populations SO JOURNAL OF VIROLOGY LA English DT Article ID PANDEMIC H1N1 2009; UNITED-STATES; HUMAN INFECTIONS; AMERICAN PIGS; TRANSMISSION; REASSORTMENT; OUTBREAK; EPIDEMIOLOGY; ARGENTINA; EMERGENCE AB The capacity of influenza A viruses to cross species barriers presents a continual threat to human and animal health. Knowledge of the human-swine interface is particularly important for understanding how viruses with pandemic potential evolve in swine hosts. We sequenced the genomes of 141 influenza viruses collected from North American swine during 2002 to 2011 and identified a swine virus that possessed all eight genome segments of human seasonal A/H3N2 virus origin. A molecular clock analysis indicates that this virus-A/sw/Saskatchewan/02903/2009(H3N2)-has likely circulated undetected in swine for at least 7 years. For historical context, we performed a comprehensive phylogenetic analysis of an additional 1,404 whole-genome sequences from swine influenza A viruses collected globally during 1931 to 2013. Human-to-swine transmission occurred frequently over this time period, with 20 discrete introductions of human seasonal influenza A viruses showing sustained onward transmission in swine for at least 1 year since 1965. Notably, human-origin hemagglutinin (H1 and H3) and neuraminidase (particularly N2) segments were detected in swine at a much higher rate than the six internal gene segments, suggesting an association between the acquisition of swine-origin internal genes via reassortment and the adaptation of human influenza viruses to new swine hosts. Further understanding of the fitness constraints on the adaptation of human viruses to swine, and vice versa, at a genomic level is central to understanding the complex multihost ecology of influenza and the disease threats that swine and humans pose to each other. IMPORTANCE The swine origin of the 2009 A/H1N1 pandemic virus underscored the importance of understanding how influenza A virus evolves in these animals hosts. While the importance of reassortment in generating genetically diverse influenza viruses in swine is well documented, the role of human-to-swine transmission has not been as intensively studied. Through a large-scale sequencing effort, we identified a novel influenza virus of wholly human origin that has been circulating undetected in swine for at least 7 years. In addition, we demonstrate that human-to-swine transmission has occurred frequently on a global scale over the past decades but that there is little persistence of human virus internal gene segments in swine. C1 [Nelson, Martha I.; Viboud, Cecile] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Wentworth, David E.; LaPointe, Matthew P.; Lin, Xudong] J Craig Venter Inst, Rockville, MD USA. [Culhane, Marie R.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. [Vincent, Amy L.] USDA ARS, Natl Anim Dis Ctr, Virus & Prion Res Unit, Ames, IA 50010 USA. [Holmes, Edward C.] Univ Sydney, Charles Perkins Ctr, Sch Biol Sci, Marie Bashir Inst Infect Dis & Biosecur, Sydney, NSW 2006, Australia. [Holmes, Edward C.] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. [Detmer, Susan E.] Univ Saskatchewan, Western Coll Vet Med, Saskatoon, SK S7N 0W0, Canada. RP Nelson, MI (reprint author), NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. EM nelsonma@mail.nih.gov OI Wentworth, David/0000-0002-5190-980X; Holmes, Edward/0000-0001-9596-3552 FU Saskatchewan Ministry of Agriculture [C70077]; Multinational Influenza Seasonal Mortality Study (MISMS); Fogarty International Center; National Institutes of Health; Office of Global Affairs at the Department of Health and Human Services; National Institute of Allergy and Infectious Diseases; National Institutes of Health, Department of Health and Human Services [HHSN272200900007C]; NHMRC Australia Fellowship FX Partial support for this work was provided by a grant from the Saskatchewan Ministry of Agriculture, contract number C70077. This work was supported in part by the Multinational Influenza Seasonal Mortality Study (MISMS), an on-going international collaborative effort to understand influenza epidemiology and evolution, led by the Fogarty International Center, National Institutes of Health, with funding from the Office of Global Affairs at the Department of Health and Human Services [MIN, CV]. This project has also been funded in part with federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under contract number HHSN272200900007C. E.C.H. is supported by an NHMRC Australia Fellowship. NR 45 TC 20 Z9 20 U1 1 U2 22 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD SEP PY 2014 VL 88 IS 17 BP 10110 EP 10119 DI 10.1128/JVI.01080-14 PG 10 WC Virology SC Virology GA AO3LW UT WOS:000341232300051 PM 24965467 ER PT J AU Morrison, J Josset, L Tchitchek, N Chang, J Belser, JA Swayne, DE Pantin-Jackwood, MJ Tumpey, TM Katze, MG AF Morrison, Juliet Josset, Laurence Tchitchek, Nicolas Chang, Jean Belser, Jessica A. Swayne, David E. Pantin-Jackwood, Mary J. Tumpey, Terrence M. Katze, Michael G. TI H7N9 and Other Pathogenic Avian Influenza Viruses Elicit a Three-Pronged Transcriptomic Signature That Is Reminiscent of 1918 Influenza Virus and Is Associated with Lethal Outcome in Mice SO JOURNAL OF VIROLOGY LA English DT Article ID BRONCHIAL EPITHELIAL-CELLS; A VIRUS; MOUSE MODEL; INCREASED VIRULENCE; RECEPTOR-BINDING; HUMAN INFECTIONS; IN-VITRO; HUMANS; FERRETS; LUNG AB Modulating the host response is a promising approach to treating influenza, caused by a virus whose pathogenesis is determined in part by the reaction it elicits within the host. Though the pathogenicity of emerging H7N9 influenza virus in several animal models has been reported, these studies have not included a detailed characterization of the host response following infection. Therefore, we characterized the transcriptomic response of BALB/c mice infected with H7N9 (A/Anhui/01/2013) virus and compared it to the responses induced by H5N1 (A/Vietnam/1203/2004), H7N7 (A/Netherlands/219/2003), and pandemic 2009 H1N1 (A/Mexico/4482/2009) influenza viruses. We found that responses to the H7 subtype viruses were intermediate to those elicited by H5N1 and pdm09H1N1 early in infection but that they evolved to resemble the H5N1 response as infection progressed. H5N1, H7N7, and H7N9 viruses were pathogenic in mice, and this pathogenicity correlated with increased transcription of cytokine response genes and decreased transcription of lipid metabolism and coagulation signaling genes. This three-pronged transcriptomic signature was observed in mice infected with pathogenic H1N1 strains such as the 1918 virus, indicating that it may be predictive of pathogenicity across multiple influenza virus strains. Finally, we used host transcriptomic profiling to computationally predict drugs that reverse the host response to H7N9 infection, and we identified six FDA-approved drugs that could potentially be repurposed to treat H7N9 and other pathogenic influenza viruses. IMPORTANCE Emerging avian influenza viruses are of global concern because the human population is immunologically naive to them. Current influenza drugs target viral molecules, but the high mutation rate of influenza viruses eventually leads to the development of antiviral resistance. As the host evolves far more slowly than the virus, and influenza pathogenesis is determined in part by the host response, targeting the host response is a promising approach to treating influenza. Here we characterize the host transcriptomic response to emerging H7N9 influenza virus and compare it with the responses to H7N7, H5N1, and pdm09H1N1. All three avian viruses were pathogenic in mice and elicited a transcriptomic signature that also occurs in response to the legendary 1918 influenza virus. Our work identifies host responses that could be targeted to treat severe H7N9 influenza and identifies six FDA-approved drugs that could potentially be repurposed as H7N9 influenza therapeutics. C1 [Morrison, Juliet; Josset, Laurence; Tchitchek, Nicolas; Chang, Jean; Katze, Michael G.] Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98195 USA. [Belser, Jessica A.; Tumpey, Terrence M.] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Influenza Div, Atlanta, GA USA. [Swayne, David E.; Pantin-Jackwood, Mary J.] USDA ARS, Southeast Poultry Res Lab, Athens, GA 30613 USA. RP Katze, MG (reprint author), Univ Washington, Sch Med, Dept Microbiol, Seattle, WA 98195 USA. EM honey@u.washington.edu RI Josset, Laurence/A-7960-2015; OI Josset, Laurence/0000-0002-7158-1186; Tchitchek, Nicolas/0000-0003-3307-0446 FU NIH; NIAID Network of Centers of Excellence in Influenza Research and Surveillance (CEIRS) [HHSN266200700008C] FX This project was funded with federal funds from the NIH, NIAID Network of Centers of Excellence in Influenza Research and Surveillance (CEIRS), under contract HHSN266200700008C. NR 72 TC 12 Z9 13 U1 0 U2 16 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD SEP PY 2014 VL 88 IS 18 BP 10556 EP 10568 DI 10.1128/JVI.00570-14 PG 13 WC Virology SC Virology GA AO3MQ UT WOS:000341234900026 PM 24991006 ER PT J AU Roman, LA Battles, JJ McBride, JR AF Roman, Lara A. Battles, John J. McBride, Joe R. TI Determinants of establishment survival for residential trees in Sacramento County, CA SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Conditional inference trees; Socio-ecological system; Survivorship; Tree mortality; Urban ecosystem ID INTERVAL-CENSORED-DATA; URBAN FOREST; ECOSYSTEM SERVICES; COST-EFFECTIVENESS; STREET TREES; AIR-QUALITY; GROWTH; CITY; MORTALITY; IMPACTS AB Urban forests can provide ecosystem services that motivate tree planting campaigns, and tree survival is a key element of program success and projected benefits. We studied survival in a shade tree give-away program in Sacramento, CA, monitoring a cohort of young trees for five years on single-family residential properties. We used conditional inference trees to identify the most important risk factors at different life history stages, and survival analysis to evaluate post-planting survivorship. Our analysis included socioeconomic, biophysical, and maintenance characteristics. In addition to field observations of tree planting status, survival, and maintenance, we also collected property ownership information through the Multiple Listing Service and neighborhood socioeconomic characteristics from the U.S. Census. We found that 84.9% of trees were planted, with 70.9% survivorship at five years post-planting. Overall, 58.9% of delivered trees survived to five years, which the local program calls survivability. Planting rates were higher in neighborhoods with higher educational attainment, and on owner-occupied properties with stable residential ownership. Five-year survival was also higher for properties with stable homeownership, as well as for tree species with low water use demand. When we incorporated maintenance characteristics from the first year of field observations, factors related to tree care were important to survival. Many residents did not adhere to recommended maintenance practices. Our results illustrate the critical role of tree care and consistent homeownership to young tree mortality on residential properties, and suggest that survival assumptions in urban forest ecosystem services models may be overly optimistic. Published by Elsevier B.V. C1 [Roman, Lara A.; Battles, John J.; McBride, Joe R.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Roman, Lara A.] USDA Forest Serv, No Res Stn, Philadelphia Field Stn, Philadelphia, PA 19103 USA. RP Roman, LA (reprint author), USDA Forest Serv, No Res Stn, Philadelphia Field Stn, 100 N 20th St,Suite 205, Philadelphia, PA 19103 USA. EM lroman@fs.fed.us; jbattles@berkeley.edu; jrm2@berkeley.edu RI Battles, John/G-8233-2012 OI Battles, John/0000-0001-7124-7893 FU SMUD; Garden Club of America's Urban Forestry Fellowship; University of California, Berkeley Schwabacher Fellowship FX This research was funded by SMUD, the Garden Club of America's Urban Forestry Fellowship and the University of California, Berkeley Schwabacher Fellowship to L.A. Roman. We thank STF (C. Cadwallader, J. Caditz, C. Blain, R. Tretheway, M. King, L. Leineke, S. Saetern) and SMUD (M. Sarkovich) staff for their time and dedication to this multi-year study. We are grateful to D. Covill and W. Miller for helping us access homeownership data in the MLS. Five summers of field work were made possible thanks to many interns (M. Chun, J. Aames, S. Robinson, C. Rankin, S. Ashton, F. Champenois, T. Haass Walpert) and volunteers (A. Hafer, J. Plummer). We thank L. Mozingo, D. Nowak, M. Russell, J. Stanovick, and three anonymous reviewers for helpful comments on the manuscript. NR 62 TC 8 Z9 8 U1 6 U2 39 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD SEP PY 2014 VL 129 BP 22 EP 31 DI 10.1016/j.landurbplan.2014.05.004 PG 10 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA AN6JE UT WOS:000340700000003 ER PT J AU Nassauer, JI Cooper, DA Marshall, LL Currie, WS Hutchins, M Brown, DG AF Nassauer, Joan Iverson Cooper, D. Ayehlet Marshall, Lauren L. Currie, William S. Hutchins, Meghan Brown, Daniel G. TI Parcel size related to household behaviors affecting carbon storage in exurban residential landscapes SO LANDSCAPE AND URBAN PLANNING LA English DT Article DE Urban ecology; Urban forestry; Sustainability; Climate change; Metropolitan; Urban planning ID UNITED-STATES; LAND-USE; SOCIOECOLOGICAL SYSTEMS; NATURAL SYSTEMS; COUPLED HUMAN; URBAN; PATTERNS; USA; PREFERENCE; VEGETATION AB We investigated whether exurban parcel size might be related to homeowners' behaviors that could affect carbon balance in residential landscapes. Based on data drawn from in-depth interviews and field surveys in 26 southeast Michigan exurban households, we conclude that an approximately 1-acre (0.45 ha) size may be a critical threshold for certain behaviors, such as leaving a portion of an exurban parcel in forest or old field or retaining leaf litter on the property. Tree planting is a relevant behavior that may increase when parcels are larger than 0.5 acres (0.20 ha). Yard style of adjacent properties also appears to be related to homeowners' landscape management behaviors: conventional turf-dominated yards prevail adjacent to parcels smaller than one-acre. We used our analysis of these data to develop an exurban homeowner typology to be employed in agent based models in which homeowners' behaviors that may affect carbon balance are linked to parcel size. (C) 2014 Elsevier B.V. All rights reserved. C1 [Nassauer, Joan Iverson; Cooper, D. Ayehlet; Currie, William S.; Hutchins, Meghan; Brown, Daniel G.] Univ Michigan, Sch Nat Resources & Environm, Ann Arbor, MI 48109 USA. [Marshall, Lauren L.] USDA Forest Serv, Kansas City, KS USA. RP Nassauer, JI (reprint author), Univ Michigan, Sch Nat Resources & Environm, 1572 Dana,430 East Univ, Ann Arbor, MI 48109 USA. EM nassauer@umich.edu; ayehlet@umich.edu; lemarshall@fs.fed.us; wgcur@umich.edu; meghan.hutchins@gmail.com; danbrown@umich.edu RI Brown, Dan/L-8089-2013 OI Brown, Dan/0000-0001-6023-5950 NR 66 TC 7 Z9 7 U1 4 U2 30 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-2046 EI 1872-6062 J9 LANDSCAPE URBAN PLAN JI Landsc. Urban Plan. PD SEP PY 2014 VL 129 BP 55 EP 64 DI 10.1016/j.landurbplan.2014.05.007 PG 10 WC Ecology; Environmental Studies; Geography; Geography, Physical; Urban Studies SC Environmental Sciences & Ecology; Geography; Physical Geography; Urban Studies GA AN6JE UT WOS:000340700000006 ER PT J AU Pachepsky, YA Blaustein, RA Whelan, G Shelton, DR AF Pachepsky, Y. A. Blaustein, R. A. Whelan, G. Shelton, D. R. TI Comparing temperature effects on Escherichia coli, Salmonella, and Enterococcus survival in surface waters SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE Escherichia coli (all potentially pathogenic types); indicators; processes; Salmonella; thermal; water quality ID INDICATOR BACTERIA; FRESH-WATER; DEPENDENCE; MODEL; INACTIVATION; TYPHIMURIUM; SEAWATER; PLANT; RATES; RIVER AB The objective of this study was to compare dependency of survival rates on temperature for indicator organisms Escherichia coli and Enterococcus and the pathogen Salmonella in surface waters. A database of 86 survival datasets from peer-reviewed papers on inactivation of E. coli, Salmonella and Enterococcus in marine waters and of E. coli and Salmonella in lake waters was assembled. The Q(10) model was used to express temperature effect on survival rates obtained from linear sections of semi-logarithmic survival graphs. Available data were insufficient to establish differences in survival rates and temperature dependencies for marine waters where values of Q(10) = 3 and a survival rate of 0.7 day(-1) could be applied. The Q(10) values in lake waters were substantially lower in marine waters, and Salmonella inactivation in lake water was, on average, twice as fast as E. coli; data on E. coli substantially outnumber data on Enterococcus and Salmonella. The relative increase in inactivation with increase in temperature is higher in marine waters than lake water, and differences in inactivation between Salmonella and E. coli at a given temperature were significant in lake water but not in marine waters. C1 [Pachepsky, Y. A.; Blaustein, R. A.; Shelton, D. R.] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville Agr Res Ctr, Beltsville, MD USA. [Whelan, G.] US EPA, Natl Exposure Res Lab, Ecosyst Res Div, Athens, GA USA. RP Pachepsky, YA (reprint author), 10300 Baltimore Ave Bldg 173, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090 FU United States Environmental Protection Agency through its Office of Research and Development [DW-12-92348101] FX The United States Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here under contract DW-12-92348101 to the USDA-ARS. It has been subjected to agency review and approved for publication. NR 31 TC 9 Z9 9 U1 5 U2 57 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD SEP PY 2014 VL 59 IS 3 BP 278 EP 283 DI 10.1111/lam.12272 PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AN6DO UT WOS:000340684400004 PM 24739086 ER PT J AU Lu, SW Faris, JD Sherwood, R Friesen, TL Edwards, MC AF Lu, Shunwen Faris, Justin D. Sherwood, Robert Friesen, Timothy L. Edwards, Michael C. TI A dimeric PR-1-type pathogenesis-related protein interacts with ToxA and potentially mediates ToxA-induced necrosis in sensitive wheat SO MOLECULAR PLANT PATHOLOGY LA English DT Article DE host-selective toxin; inducible defence-related protein; protein dimerization; protein-protein interaction ID PYRENOPHORA-TRITICI-REPENTIS; PTR TOXA; NECROTROPHIC PATHOGENS; STAGONOSPORA-NODORUM; TRANSGENIC TOBACCO; SELECTIVE TOXIN; GENE FAMILY; BASIC PR-1; TAN SPOT; PLANT AB A dimeric PR-1-type pathogenesis-related protein (PR-1-5), recently identified in wheat, was found to interact with Stagonospora nodorum ToxA in both yeast two-hybrid and co-immunoprecipitation assays. Site-specific mutational analyses revealed that the RGD motif of ToxA is not targeted by PR-1-5, whereas two surface-exposed asparagine residues are essential for the interaction: the N102 residue of the turning loop between beta 2 and beta 3 in ToxA and the N141 residue of the turning loop between beta C and beta D in PR-1-5. Recombinant PR-1-5 and ToxA mutant proteins carrying alanine substitutions at the interacting sites were expressed in Pichia pastoris, together with the wildtype proteins. Native polyacrylamide gel electrophoresis (PAGE) confirmed that the PR-1-5-N141A mutant retains the ability to form dimers. Plant assays indicated that the ToxA-N102A mutant fails to induce necrosis, whereas the PR-1-5-N141A mutant is impaired in the ` necrosis-promoting' activity shown by the wild-type PR-1-5 when co-infiltrated with ToxA in sensitive wheat. Reverse transcriptase-polymerase chain reaction and Western blot analyses revealed that the native PR-1-5 protein is differentially expressed between ToxA-sensitive and ToxA-insensitive wheat lines in response to ToxA treatment. These results suggest that PR-1-5 is a potential target of ToxA and the site-specific interaction between PR-1-5 and ToxA may mediate ToxA-induced necrosis in sensitive wheat. C1 [Lu, Shunwen; Faris, Justin D.; Friesen, Timothy L.; Edwards, Michael C.] USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. [Sherwood, Robert] Cornell Univ, Biotechnol Resource Ctr, Ithaca, NY 14853 USA. RP Lu, SW (reprint author), USDA ARS, Cereal Crops Res Unit, Fargo, ND 58102 USA. EM shunwen.lu@ars.usda.gov FU USDA-ARS CRIS [5442-21000-037-00D] FX We thank Kelsey Dunnell and Tyler Lewandowski for technical assistance. This study was supported by USDA-ARS CRIS project 5442-21000-037-00D. NR 71 TC 8 Z9 8 U1 0 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD SEP PY 2014 VL 15 IS 7 BP 650 EP 663 DI 10.1111/mpp.12122 PG 14 WC Plant Sciences SC Plant Sciences GA AN6BC UT WOS:000340677400002 PM 24433289 ER PT J AU Ali, SS Melnick, RL Crozier, J Phillips-Mora, W Strem, MD Shao, J Zhang, DP Sicher, R Meinhardt, L Bailey, BA AF Ali, Shahin S. Melnick, Rachel L. Crozier, Jayne Phillips-Mora, Wilberth Strem, Mary D. Shao, Jonathan Zhang, Dapeng Sicher, Richard Meinhardt, Lyndel Bailey, Bryan A. TI Successful pod infections by Moniliophthora roreri result in differential Theobroma cacao gene expression depending on the clone's level of tolerance SO MOLECULAR PLANT PATHOLOGY LA English DT Article DE differential transcriptome; frosty pod rot; hemibiotroph; plant defence; RNA-Seq; tolerance ID WRKY TRANSCRIPTION FACTORS; PLANT-DISEASE RESISTANCE; WITCHES-BROOM DISEASE; ENHANCES RESISTANCE; ABSCISIC-ACID; PATHOGEN; ETHYLENE; PROTEIN; TOMATO; HEAT-SHOCK-PROTEIN-90 AB An understanding of the tolerance mechanisms of Theobroma cacao used against Moniliophthora roreri, the causal agent of frosty pod rot, is important for the generation of stable diseasetolerant clones. A comparative view was obtained of transcript populations of infected pods from two susceptible and two tolerant clones using RNA sequence (RNA-Seq) analysis. A total of 3009 transcripts showed differential expression among clones. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis of differentially expressed genes indicated shifts in 152 different metabolic pathways between the tolerant and susceptible clones. Real-time quantitative reverse transcription polymerase chain reaction (real-time qRT-PCR) analyses of 36 genes verified the differential expression. Regression analysis validated a uniform progression in gene expression in association with infection levels and fungal loads in the susceptible clones. Expression patterns observed in the susceptible clones diverged in tolerant clones, with many genes showing higher expression at a low level of infection and fungal load. Principal coordinate analyses of real-time qRT-PCR data separated the gene expression patterns between susceptible and tolerant clones for pods showing malformation. Although some genes were constitutively differentially expressed between clones, most results suggested that defence responses were induced at low fungal load in the tolerant clones. Several elicitor-responsive genes were highly expressed in tolerant clones, suggesting rapid recognition of the pathogen and induction of defence genes. Expression patterns suggested that the jasmonic acid-ethylene-and/or salicylic acid-mediated defence pathways were activated in the tolerant clones, being enhanced by reduced brassinosteroid (BR) biosynthesis and catabolic inactivation of both BR and abscisic acids. Finally, several genes associated with hypersensitive response-like cell death were also induced in tolerant clones. C1 [Ali, Shahin S.; Melnick, Rachel L.; Strem, Mary D.; Zhang, Dapeng; Meinhardt, Lyndel; Bailey, Bryan A.] USDA ARS, Sustainable Perennial Crops Lab, Inst Plant Sci, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Crozier, Jayne] CATIE, CABI Caribbean & Latin Amer CATIE Off, Turrialba 7170, Costa Rica. [Phillips-Mora, Wilberth] CATIE, Dept Agr & Agroforesteria, Turrialba 7170, Costa Rica. [Shao, Jonathan] USDA ARS, Mol Plant Pathol Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Sicher, Richard] USDA ARS, Crop Syst & Global Change Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. RP Bailey, BA (reprint author), USDA ARS, Sustainable Perennial Crops Lab, Inst Plant Sci, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. EM bryan.bailey@ars.usda.gov FU United States Department of Agriculture Agricultural Research Service (USDA ARS) FX This work was funded by the United States Department of Agriculture Agricultural Research Service (USDA ARS). References to a company and/or product by the USDA are only for the purposes of information and do not imply approval or recommendation of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 57 TC 4 Z9 4 U1 0 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD SEP PY 2014 VL 15 IS 7 BP 698 EP 710 DI 10.1111/mpp.12126 PG 13 WC Plant Sciences SC Plant Sciences GA AN6BC UT WOS:000340677400006 PM 24528440 ER PT J AU Bailey, BA Melnick, RL Strem, MD Crozier, J Shao, J Sicher, R Phillips-Mora, W Ali, SS Zhang, DP Meinhardt, L AF Bailey, Bryan A. Melnick, Rachel L. Strem, Mary D. Crozier, Jayne Shao, Jonathan Sicher, Richard Phillips-Mora, Wilberth Ali, Shahin S. Zhang, Dapeng Meinhardt, Lyndel TI Differential gene expression by Moniliophthora roreri while overcoming cacao tolerance in the field SO MOLECULAR PLANT PATHOLOGY LA English DT Article DE disease tolerance; frosty pod rot; hemibiotroph; metabolite; Moniliophthora roreri; RNA-Seq; Theobroma cacao ID WITCHES-BROOM DISEASE; THEOBROMA-CACAO; SACCHAROMYCES-CEREVISIAE; FILAMENTOUS FUNGI; CAUSAL AGENT; FROSTY POD; PERNICIOSA; PROTEINS; HYDROPHOBINS; RESISTANCE AB Frosty pod rot (FPR) of Theobroma cacao (cacao) is caused by the hemibiotrophic fungus Moniliophthora roreri. Cacao clones tolerant to FPR are being planted throughout Central America. To determine whether M. roreri shows a differential molecular response during successful infections of tolerant clones, we collected field-infected pods at all stages of symptomatology for two highly susceptible clones (Pound-7 and CATIE-1000) and three tolerant clones (UF-273, CATIE-R7 and CATIE-R4). Metabolite analysis was carried out on clones Pound-7, CATIE-1000, CATIE-R7 and CATIE-R4. As FPR progressed, the concentrations of sugars in pods dropped, whereas the levels of trehalose and mannitol increased. Associations between symptoms and fungal loads and some organic and amino acid concentrations varied depending on the clone. RNA-Seq analysis identified 873 M. roreri genes that were differentially expressed between clones, with the primary difference being whether the clone was susceptible or tolerant. Genes encoding transcription factors, heat shock proteins, transporters, enzymes modifying membranes or cell walls and metabolic enzymes, such as malate synthase and alternative oxidase, were differentially expressed. The differential expression between clones of 43 M. roreri genes was validated by real-time quantitative reverse transcription polymerase chain reaction. The expression profiles of some genes were similar in susceptible and tolerant clones (other than CATIE-R4) and varied with the biotrophic/necrotropic shift. Moniliophthora roreri genes associated with stress metabolism and responses to heat shock and anoxia were induced early in tolerant clones, their expression profiles resembling that of the necrotrophic phase. Moniliophthora roreri stress response genes, induced during the infection of tolerant clones, may benefit the fungus in overcoming cacao defense mechanisms. C1 [Bailey, Bryan A.; Melnick, Rachel L.; Strem, Mary D.; Shao, Jonathan; Ali, Shahin S.; Zhang, Dapeng; Meinhardt, Lyndel] USDA ARS, Sustainable Perennial Crops Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Crozier, Jayne] CATIE, CABI Caribbean & Latin Amer CATIE Off, Turrialba 7170, Costa Rica. [Sicher, Richard] USDA ARS, Crop Syst & Global Change Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. [Phillips-Mora, Wilberth] CATIE, Dept Agr & Agroforesteria, Turrialba 7170, Costa Rica. RP Bailey, BA (reprint author), USDA ARS, Sustainable Perennial Crops Lab, Beltsville Agr Res Ctr West, Beltsville, MD 20705 USA. EM bryan.bailey@ars.usda.gov FU US Department of Agriculture Agricultural Research Service (USDA-ARS) FX This work was funded by the US Department of Agriculture Agricultural Research Service (USDA-ARS). References to a company and/or product by the USDA are only for the purposes of information and do not imply approval or recommendation of the product to the exclusion of others that may also be suitable. USDA is an equal opportunity provider and employer. NR 57 TC 7 Z9 7 U1 4 U2 33 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1464-6722 EI 1364-3703 J9 MOL PLANT PATHOL JI Mol. Plant Pathol. PD SEP PY 2014 VL 15 IS 7 BP 711 EP 729 DI 10.1111/mpp.12134 PG 19 WC Plant Sciences SC Plant Sciences GA AN6BC UT WOS:000340677400007 PM 24612180 ER PT J AU Johnson, EJ AF Johnson, Elizabeth J. TI Role of lutein and zeaxanthin in visual and cognitive function throughout the lifespan SO NUTRITION REVIEWS LA English DT Review DE carotenoid; cognition; lifespan; lutein; vision; zeaxanthin ID PIGMENT OPTICAL-DENSITY; HUMAN MACULAR PIGMENT; OLDER WOMEN; EYE DISEASE; VITAMIN-A; NUTRITIONAL MANIPULATION; VISION IMPAIRMENT; ALZHEIMER-DISEASE; PRIMATE RETINAS; DIETARY LUTEIN AB The relationship between lutein and zeaxanthin and visual and cognitive health throughout the lifespan is compelling. There is a variety of evidence to support a role for lutein and zeaxanthin in vision. Lutein's role in cognition has only recently been considered. Lutein and its isomer, zeaxanthin, are taken up selectively into eye tissue. Lutein is the predominant carotenoid in human brain tissue. Lutein and zeaxanthin in neural tissue may have biological effects that include antioxidation, anti-inflammation, and structural actions. In addition, lutein and zeaxanthin may be protective against eye disease because they absorb damaging blue light that enters the eye. In pediatric brains, the relative contribution of lutein to the total carotenoids is twice that found in adults, accounting for more than half the concentration of total carotenoids. The greater proportion of lutein in the pediatric brain suggests a need for lutein during neural development as well. In adults, higher lutein status is related to better cognitive performance, and lutein supplementation improves cognition. The evidence to date warrants further investigation into the role of lutein and zeaxanthin in visual and cognitive health throughout the lifespan. (C) 2014 International Life Sciences Institute C1 [Johnson, Elizabeth J.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Antioxidants Res Lab, Boston, MA 02111 USA. [Johnson, Elizabeth J.] Tufts Univ, Gerald J & Dorothy Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Johnson, EJ (reprint author), Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM elizabeth.johnson@tufts.edu FU US Department of Agriculture [58-1950-7-707] FX This manuscript was supported by a US Department of Agriculture grant (no. 58-1950-7-707) and an unrestricted gift from OmniActive Health Technologies, Inc. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author and do not necessarily reflect the view of the US Department of Agriculture or OmniActive Health Technologies, Inc. NR 85 TC 30 Z9 30 U1 5 U2 52 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0029-6643 EI 1753-4887 J9 NUTR REV JI Nutr. Rev. PD SEP PY 2014 VL 72 IS 9 BP 605 EP 612 DI 10.1111/nure.12133 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AO3ML UT WOS:000341234300005 PM 25109868 ER PT J AU Smith, SM Castaneda-Sceppa, C O'Brien, KO Abrams, SA Gillman, P Brooks, NE Cloutier, GJ Heer, M Zwart, SR Wastney, ME AF Smith, S. M. Castaneda-Sceppa, C. O'Brien, K. O. Abrams, S. A. Gillman, P. Brooks, N. E. Cloutier, G. J. Heer, M. Zwart, S. R. Wastney, M. E. TI Calcium kinetics during bed rest with artificial gravity and exercise countermeasures SO OSTEOPOROSIS INTERNATIONAL LA English DT Article DE Biochemical markers of bone turnover; Exercise; Microgravity; Nutrition; Space flight ID INDUCED BONE LOSS; AMINO-ACID SUPPLEMENTATION; RESISTANCE EXERCISE; SPACE-FLIGHT; ENERGY DEFICIT; METABOLISM; SPACEFLIGHT; WEIGHTLESSNESS; RESORPTION; RECOVERY AB We assessed the potential for countermeasures to lessen the loss of bone calcium during bed rest. Subjects ingested less calcium during bed rest, and with artificial gravity, they also absorbed less calcium. With exercise, they excreted less calcium. To retain bone during bed rest, calcium intake needs to be maintained. This study aims to assess the potential for artificial gravity (AG) and exercise (EX) to mitigate loss of bone calcium during space flight. We performed two studies: (1) a 21-day bed rest (BR) study with subjects receiving 1 h/day AG (n = 8) or no AG (n = 7) and (2) a 28-day BR study with 1 h/day resistance EX (n = 10) or no EX (n = 3). In both studies, stable isotopes of Ca were administered orally and intravenously, at baseline and after 10 days of BR, and blood, urine, and feces were sampled for up to 14 days post dosing. Tracers were measured using thermal ionization mass spectrometry. Data were analyzed by compartmental modeling. Less Ca was absorbed during BR, resulting in lower Ca balance in BR+AG (-6.04 +/- 3.38 mmol/day, P = 0.023). However, Ca balance did not change with BR+EX, even though absorbed Ca decreased and urinary Ca excretion increased, because endogenous excretion decreased, and there was a trend for increased bone deposition (P = 0.06). Urinary N-telopeptide excretion increased in controls during BR, but not in the EX group. Markers of bone formation were not different between treatment groups for either study. Ca intake decreased during BR (by 5.4 mmol/day in the AG study and 2.8 mmol/day in the EX study), resulting in lower absorbed Ca. During BR (or space flight), Ca intake needs to be maintained or even increased with countermeasures such as exercise, to enable maintenance of bone Ca. C1 [Smith, S. M.] NASA, Lyndon B Johnson Space Ctr, Houston, TX 77058 USA. [Castaneda-Sceppa, C.; Cloutier, G. J.] Northeastern Univ, Boston, MA 02115 USA. [O'Brien, K. O.] Cornell Univ, Ithaca, NY USA. [Abrams, S. A.] ARS, Baylor Coll Med, USDA, Childrens Nutr Res Ctr, Houston, TX USA. [Gillman, P.] EASI, Houston, TX USA. [Brooks, N. E.] Univ Stirling, Stirling FK9 4LA, Scotland. [Heer, M.] Univ Bonn, Bonn, Germany. [Zwart, S. R.] Univ Space Res Assoc, Houston, TX USA. [Wastney, M. E.] Metab Modeling, W Lafayette, IN USA. RP Smith, SM (reprint author), NASA, Lyndon B Johnson Space Ctr, Attn Mail Code SK3,2101 NASA Pkwy, Houston, TX 77058 USA. EM scott.m.smith@nasa.gov OI Abrams, Steven/0000-0003-4972-9233 FU NASA Flight Analogs Project of NASA's Human Research Program; National Center for Advancing Translational Sciences, National Institutes of Health [1UL1RR029876-01]; National Space Biomedical Research Institute (NSBRI) [NCC 9-58]; U.S. Department of Agriculture's Agricultural Research Service [58-1950-9-001]; National Institutes of Health GCRC [M01-RR-000054] FX We thank the subjects for their time and willingness to participate in these studies. We thank the staff of the UTMB Institute for Translational Sciences Clinical Research Center for their assistance with the AG study and the Tufts University GCRC for their support in conducting the exercise study. We thank the staff of the U.S. National Aeronautics and Space Administration (NASA) Johnson Space Center Nutritional Biochemistry Laboratory for their assistance in processing and analyzing the samples and in all aspects of carrying out this project. We thank Jane Krauhs for editorial assistance. The AG study was funded in part by the NASA Flight Analogs Project of NASA's Human Research Program and in part by grant 1UL1RR029876-01 from the National Center for Advancing Translational Sciences, National Institutes of Health. The exercise study was supported by the National Space Biomedical Research Institute (NSBRI) through NCC 9-58, by agreement 58-1950-9-001 from the U.S. Department of Agriculture's Agricultural Research Service, and by a grant M01-RR-000054 from the National Institutes of Health GCRC. NR 26 TC 2 Z9 2 U1 2 U2 7 PU SPRINGER LONDON LTD PI LONDON PA 236 GRAYS INN RD, 6TH FLOOR, LONDON WC1X 8HL, ENGLAND SN 0937-941X EI 1433-2965 J9 OSTEOPOROSIS INT JI Osteoporosis Int. PD SEP PY 2014 VL 25 IS 9 BP 2237 EP 2244 DI 10.1007/s00198-014-2754-x PG 8 WC Endocrinology & Metabolism SC Endocrinology & Metabolism GA AN6AK UT WOS:000340675200010 PM 24861908 ER PT J AU Colegate, SM Gardner, DR Betz, JM Panter, KE AF Colegate, Steven M. Gardner, Dale R. Betz, Joseph M. Panter, Kip E. TI Semi-automated Separation of the Epimeric Dehydropyrrolizidine Alkaloids Lycopsamine and Intermedine: Preparation of their N-oxides and NMR Comparison with Diastereoisomeric Rinderine and Echinatine SO PHYTOCHEMICAL ANALYSIS LA English DT Article DE Boronated soda glass chromatography; HPLC-ESI/MS; NMR; optical rotation; echinatine; dehydropyrrolizidine alkaloids; intermedine; lycopsamine; rinderine; comfrey; Symphytum officinale ID PYRROLIZIDINE ALKALOIDS; COUNTERCURRENT CHROMATOGRAPHY; SENECIO-RIDDELLII; AMSINCKIA; MS; EXTRACTION; TOXICITY; INDICINE; ANALOGS AB Introduction - The diversity of structure and, particularly, stereochemical variation of the dehydropyrrolizidine alkaloids can present challenges for analysis and the isolation of pure compounds for the preparation of analytical standards and for toxicology studies. Objective - To investigate methods for the separation of gram-scale quantities of the epimeric dehydropyrrolizidine alkaloids lycopsamine and intermedine and to compare their NMR spectroscopic data with those of their heliotridine-based analogues echinatine and rinderine. Methods - Lycopsamine and intermedine were extracted, predominantly as their N-oxides and along with their acetylated derivatives, from commercial samples of comfrey (Symphytum officinale) root. Alkaloid enrichment involved liquid-liquid partitioning of the crude methanol extract between dilute aqueous acid and n-butanol, reduction of N-oxides and subsequent continuous liquid-liquid extraction of free base alkaloids into CHCl3. The alkaloid-rich fraction was further subjected to semi-automated flash chromatography using boronated soda glass beads or boronated quartz sand. Results - Boronated soda glass beads (or quartz sand) chromatography adapted to a Biotage Isolera Flash Chromatography System enabled large-scale separation (at least up to 1-2 g quantities) of lycopsamine and intermedine. The structures were confirmed using one- and two-dimensional H-1- and C-13-NMR spectroscopy. Examination of the NMR data for lycopsamine, intermedine and their heliotridine-based analogues echinatine and rinderine allowed for some amendments of literature data and provided useful comparisons for determining relative configurations in monoester dehydropyrrolizidine alkaloids. A similar NMR comparison of lycopsamine and intermedine with their N-oxides showed the effects of N-oxidation on some key chemical shifts. A levorotatory shift in specific rotation from +3.29 degrees to -1.5 degrees was observed for lycopsamine when dissolved in ethanol or methanol respectively. Conclusion - A semi-automated flash chromatographic process using boronated soda glass beads was standardised and confirmed as a useful, larger scale preparative approach for separating the epimers lycopsamine and intermedine. The useful NMR correlations to stereochemical arrangements within this specific class of dehydropyrrolizidine alkaloid cannot be confidently extrapolated to other similar dehydropyrrolizidine alkaloids. Published 2014. This article is a U. S. Government work and is in the public domain in the USA. C1 [Colegate, Steven M.; Gardner, Dale R.; Panter, Kip E.] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Betz, Joseph M.] NIH, Off Dietary Supplements, Bethesda, MD 20892 USA. RP Colegate, SM (reprint author), ARS, Poisonous Plant Res Lab, USDA, 1150 E 1400 N, Logan, UT 84341 USA. EM steven.colegate@ars.usda.gov NR 31 TC 7 Z9 7 U1 1 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0958-0344 EI 1099-1565 J9 PHYTOCHEM ANALYSIS JI Phytochem. Anal. PD SEP-OCT PY 2014 VL 25 IS 5 BP 429 EP 438 DI 10.1002/pca.2511 PG 10 WC Biochemical Research Methods; Plant Sciences; Chemistry, Analytical SC Biochemistry & Molecular Biology; Plant Sciences; Chemistry GA AO2KD UT WOS:000341149500006 PM 24816769 ER PT J AU Beck, JJ Mahoney, NE Cook, D Gee, WS Baig, N Higbee, BS AF Beck, John J. Mahoney, Noreen E. Cook, Daniel Gee, Wai S. Baig, Nausheena Higbee, Bradley S. TI Comparison of the volatile emission profiles of ground almond and pistachio mummies: Part 1-Addressing a gap in knowledge of current attractants for navel orangeworm SO PHYTOCHEMISTRY LETTERS LA English DT Article DE Almond; Host plant volatiles; Mummies; Navel orangeworm; Pistachio; Semiochemicals ID PYRALIDAE EGG TRAPS; LEPIDOPTERA-PYRALIDAE; AMYELOIS-TRANSITELLA; CALIFORNIA; MOTHS; BAIT; ATTRACTIVENESS; ORCHARDS; DAMAGE; ACID AB Over the years various tissues of almond and pistachio have been evaluated for their ability to attract the navel orangeworm moth, a major insect pest in California tree nut orchards. Almond meal, which typically consists of ground almond kernels, is a monitoring tool for navel orangeworm populations in almond and pistachio orchards. Recently, ground pistachio and almond mummies have been re-visited in field trapping studies for their potential to attract navel orangeworm moths. Surprisingly, the volatile profiles of these two systems have not been reported. The objective of this study was to survey and then compare and contrast the volatile profiles of both ground almond and pistachio mummies over the course of a week. Principal component analysis (PCA) of the headspace volatiles showed a distinct difference between ground almond and pistachio mummies. The volatile emission profile of the almond mummies remained consistent over the one-week period, albeit in low content and composition; whereas the profile of the pistachio mummies changed over time as shown by PCA. Seven compounds were identified as being common volatiles to both matrices. These data may help explain results from recent navel orangeworm field trapping studies using almond and pistachio mummies, as well as with the formulation of future synthetic blends. Published by Elsevier Ireland Ltd on behalf of Phytochemical Society of Europe. C1 [Beck, John J.; Mahoney, Noreen E.; Gee, Wai S.; Baig, Nausheena] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Cook, Daniel] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Higbee, Bradley S.] Paramount Farming Co, Bakersfield, CA 93308 USA. RP Beck, JJ (reprint author), ARS, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM john.beck@ars.usda.gov NR 35 TC 2 Z9 2 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3900 EI 1876-7486 J9 PHYTOCHEM LETT JI Phytochem. Lett. PD SEP PY 2014 VL 9 BP 102 EP 106 DI 10.1016/j.phytol.2014.04.010 PG 5 WC Plant Sciences; Chemistry, Medicinal SC Plant Sciences; Pharmacology & Pharmacy GA AN8VR UT WOS:000340884100020 ER PT J AU Chang, HX Miller, LA Hartman, GL AF Chang, Hao-Xun Miller, Lou Ann Hartman, Glen L. TI Melanin-Independent Accumulation of Turgor Pressure in Appressoria of Phakopsora pachyrhizi SO PHYTOPATHOLOGY LA English DT Article ID SOYBEAN RUST; RICE BLAST; MAGNAPORTHE-GRISEA; HOST PENETRATION; BIOSYNTHESIS; INFECTION; FUNGICIDE; LEAVES; FUNGUS; FORCE AB Appressoria of some plant-pathogenic fungi accumulate turgor pressure that produces a mechanical force enabling the direct penetration of hyphae through the epidermis. Melanin functions as an impermeable barrier to osmolytes, which allows appressoria to accumulate high turgor pressure. Deficiency of melanin in appressoria reduces turgor pressure and compromises the infection process. In Phakopsora pachyrhizi, the soybean rust pathogen, the appressoria are hyaline. Our objective was to ensure the absence of a melanin layer specifically between the appressorial cell wall and plasma membrane, as well as to determine the turgor pressure of P. pachyrhizi appressoria. We demonstrated that two melanin biosynthesis inhibitors neither reduced turgor pressure nor compromised the infection process. Transmission electron microscopy also showed the absence of a melanin layer between the appressorial cell wall and plasma membrane. In addition, the turgor pressure of P. pachyrhizi appressoria was 5 to 6 MPa, based on extracellular osmolytes used to simulate different osmotic pressures. This is the first report showing that turgor pressure accumulation of P. pachyrhizi appressoria was independent of melanin. C1 [Chang, Hao-Xun; Hartman, Glen L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Miller, Lou Ann] Univ Illinois, Frederick Seitz Mat Res Lab, Urbana, IL 61801 USA. [Hartman, Glen L.] USDA ARS, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Chang, Hao-Xun/0000-0002-4667-7741 NR 37 TC 3 Z9 4 U1 2 U2 30 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD SEP PY 2014 VL 104 IS 9 BP 977 EP 984 DI 10.1094/PHYTO-12-13-0335-R PG 8 WC Plant Sciences SC Plant Sciences GA AN6VR UT WOS:000340737000009 PM 24779353 ER PT J AU Stark, LR Greenwood, JL Brinda, JC Oliver, MJ AF Stark, L. R. Greenwood, J. L. Brinda, J. C. Oliver, M. J. TI Physiological history may mask the inherent inducible desiccation tolerance strategy of the desert moss Crossidium crassinerve SO PLANT BIOLOGY LA English DT Article DE Acclimation; chlorosis; chlorophyll fluorescence; deacclimation; dehardening; slow-dry; constitutive desiccation tolerance ID TORTULA-RURALIS GAMETOPHYTES; PROTEIN-SYNTHESIS; ATRICHUM-ANDROGYNUM; ABSCISIC-ACID; PHYSCOMITRELLA-PATENS; CHLOROPHYLL FLUORESCENCE; PLANT DESICCATION; POLYTRICHUM-FORMOSUM; BRYUM-ARGENTEUM; ION LEAKAGE AB Shoots of bryophytes collected in the desiccated state from the field are likely to be hardened to desiccation tolerance (DT) to varying degrees. To account for this, most studies on DT include a relatively short deacclimation period. However, no study has experimentally determined the appropriate deacclimation time for any bryophyte species. Our purposes are to (i) determine if 'field effects' are biologically relevant to DT studies and how long a deacclimation period is required to remove them; and (ii) utilise field versus cultured shoot responses within the context of a deacclimation period to elucidate the ecological strategy of DT. Our hypothesis (based on an extensive literature on DT) is that a deacclimation period from 24 to 72 h should be sufficient to eliminate historical stress effects on the physiology of the shoots and allow an accurate determination of the inherent ecological DT strategy (constitutive or inducible). We determined, however, using chlorophyll fluorescence and visual estimates of shoot damage, that field-collected shoots of the desert moss Crossidium crassinerve required an experimental deacclimation period of >7 days before field effects were removed, and revealed an ecological DT strategy of inducible DT. If the deacclimation period was <6 days, the shoot response conformed to an ecological strategy of constitutive protection. Thus the presence of field effects can obscure the ecological strategy of desiccation tolerance exhibited by the species, and this translates into a need to re-evaluate previous mechanistic and ecological studies of desiccation tolerance in plants. C1 [Stark, L. R.; Greenwood, J. L.] Univ Nevada, Sch Life Sci, Las Vegas, NV 89154 USA. [Brinda, J. C.] Missouri Bot Garden, St Louis, MO USA. [Oliver, M. J.] Univ Missouri, USDA, ARS, MWA,PGRU, Columbia, MO USA. RP Stark, LR (reprint author), Univ Nevada, Sch Life Sci, 4505 Maryland Pkwy, Las Vegas, NV 89154 USA. EM LRS@UNLV.nevada.edu OI Brinda, John/0000-0001-9083-1235 NR 113 TC 7 Z9 7 U1 1 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1435-8603 EI 1438-8677 J9 PLANT BIOLOGY JI Plant Biol. PD SEP PY 2014 VL 16 IS 5 BP 935 EP 946 DI 10.1111/plb.12140 PG 12 WC Plant Sciences SC Plant Sciences GA AN6DD UT WOS:000340683100010 PM 24397604 ER PT J AU Krugner, R Sisterson, MS Chen, JC Stenger, DC Johnson, MW AF Krugner, Rodrigo Sisterson, Mark S. Chen, Jianchi Stenger, Drake C. Johnson, Marshall W. TI Evaluation of Olive as a Host of Xylella fastidiosa and Associated Sharpshooter Vectors SO PLANT DISEASE LA English DT Article ID ALMOND LEAF SCORCH; HOMALODISCA-COAGULATA HEMIPTERA; GLASSY-WINGED SHARPSHOOTER; CITRUS VARIEGATED CHLOROSIS; SAN-JOAQUIN VALLEY; PIERCES-DISEASE; SOUTHERN CALIFORNIA; AXENIC CULTURE; PHONY DISEASE; CAUSAL AGENT AB Olive (Olea europaea) trees exhibiting leaf scorch or branch dieback symptoms in California were surveyed for the xylem-limited, fastidious bacterium Xylella fastidiosa. Only approximately 17% of diseased trees tested positive for X. fastidiosa by polymerase chain reaction, and disease symptoms could not be attributed to X. fastidiosa infection of olive in greenhouse pathogenicity assays. Six strains of X. fastidiosa were isolated from olive in Southern California. Molecular assays identified strains recovered from olive as belonging to X. fastidiosa subsp. multiplex. Pathogenicity testing of olive strains on grapevine and almond confirmed that X. fastidiosa strains isolated from olive yield disease phenotypes on almond and grapevine typical of those expected for subsp. multiplex. Mechanical inoculation of X. fastidiosa olive strains to olive resulted in infection at low efficiency but infections remained asymptomatic and tended to be self-limiting. Vector transmission assays demonstrated that glassy-winged sharpshooter (Homalodisca vitripennis) could transmit strains of both subspp. multiplex and fastidiosa to olive at low efficiency. Insect trapping data indicated that two vectors of X. fastidiosa, glassy-winged sharpshooter and green sharpshooter (Draeculacephala minerva), were active in olive orchards. Collectively, the data indicate that X. fastidiosa did not cause olive leaf scorch or branch dieback but olive may contribute to the epidemiology of X. fastidiosa-elicited diseases in California. Olive may serve as an alternative, albeit suboptimal, host of X. fastidiosa. Olive also may be a refuge where sharpshooter vectors evade intensive areawide insecticide treatment of citrus, the primary control method used in California to limit glassy-winged sharpshooter populations and, indirectly, epidemics of Pierce's disease of grapevine. C1 [Krugner, Rodrigo; Sisterson, Mark S.; Chen, Jianchi; Stenger, Drake C.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Johnson, Marshall W.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. RP Krugner, R (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. EM rodrigo.krugner@ars.usda.gov FU California Olive Committee; United States Department of Agriculture-Agricultural Research Service [5302-22000-010-00D] FX This research was supported by a California Olive Committee grant awarded to R. Krugner and by the United States Department of Agriculture-Agricultural Research Service appropriated project 5302-22000-010-00D. We thank T. de la Torre, M. Venegaz, and A. J. Salyers for assisting in plant and insect maintenance; G. Phillips for maintaining bacterial cultures and assisting isolation; P. Dwyer for collection of field trapping data; and Duarte nursery for donating grapevines. NR 53 TC 15 Z9 18 U1 4 U2 57 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1186 EP 1193 DI 10.1094/PDIS-01-14-0014-RE PG 8 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300002 ER PT J AU Palumbo, JD Mahoney, NE Light, DM Siegel, J Puckett, RD Michailides, TJ AF Palumbo, Jeffrey D. Mahoney, Noreen E. Light, Douglas M. Siegel, Joel Puckett, Ryan D. Michailides, Themis J. TI Spread of Aspergillus flavus by Navel Orangeworm (Amyelois transitella) on Almond SO PLANT DISEASE LA English DT Article ID LEPIDOPTERA; AFLATOXINS; CALIFORNIA; PYRALIDAE; CORN AB Navel orangeworm (NOW) damage to almond is correlated with increased incidence of aflatoxin contamination caused by Aspergillus flavus. However, no reports demonstrate a causative relationship between NOW feeding and A. flavus infection. To demonstrate the potential of NOW to act as a vector of A. flavus on almond, NOW eggs were dusted with A. flavus and incubated in microchambers adjacent to but not touching agar plates or almond kernels. Following egg hatch, A. flavus colonies developed on agar along trails left by NOW larvae. Almond kernels damaged with A. flavus-carrying NOW showed higher incidence of A. flavus colonization and aflatoxin contamination than control treatments. Interestingly, levels of aflatoxin in NOW-damaged, A. flavus-infected almond were significantly higher than control treatments, even in the absence of visible fungal growth. Commercial almond orchards had a relatively low level of contamination with Aspergillus section Flavi in spring and early summer and a high level during summer, corresponding with the higher level of NOW infestation of the crop. Our study demonstrates that NOW is capable of vectoring A. flavus to almond, and that monitoring and sorting of almond kernels for insect damage is warranted to limit aflatoxin contamination potential both before and after harvesting. C1 [Palumbo, Jeffrey D.; Mahoney, Noreen E.; Light, Douglas M.] USDA ARS, Plant Mycotoxin Res Unit, Western Reg Res Ctr, Albany, CA 94710 USA. [Siegel, Joel] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Puckett, Ryan D.; Michailides, Themis J.] Univ Calif Davis, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. RP Michailides, TJ (reprint author), Univ Calif Davis, Kearney Agr Res & Extens Ctr, Parlier, CA 93648 USA. EM tjmichailides@ucanr.edu FU Almond Board of California; USDA Agricultural Research Service CRIS project [5325-42000-036-00D]; USDA-ARS Area-wide Project for Navel Orangeworm Control in Almonds, Pistachios, and Walnuts FX This work was supported by the Almond Board of California, the USDA Agricultural Research Service CRIS project number 5325-42000-036-00D, and the USDA-ARS Area-wide Project for Navel Orangeworm Control in Almonds, Pistachios, and Walnuts. We thank B. Campbell, M. Jacobs, T. Birmingham, R. Curtis, and A. Craven for helpful discussions; and D. Brandl for providing NOW materials. NR 23 TC 8 Z9 8 U1 0 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1194 EP 1199 DI 10.1094/PDIS-09-13-1015-RE PG 6 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300003 ER PT J AU Ma, JB Jia, MH Jia, Y AF Ma, Jianbing Jia, M. H. Jia, Y. TI Characterization of Rice Blast Resistance Gene Pi61(t) in Rice Germplasm SO PLANT DISEASE LA English DT Article ID ORYZA-SATIVA L.; PI-TA; MAGNAPORTHE-ORYZAE; SSR MARKERS; DNA MARKERS; LOCUS; IDENTIFICATION; SEQUENCE; GENOME; INDICA AB Identification of resistance (R) genes to races of Magnaporthe oryzae in rice (Oryza sativa) germplasm is essential for the development of rice cultivars with long-lasting blast resistance. In the present study, one major quantitative trait locus, qPi93-3, was fine mapped using a recombinant inbred line (RIL), F-8 RIL171, derived from the cross between 'Nipponbare' and '93-11'. RIL171 contained a heterozygous qPi93-3 allele which was found to be resistant against nine U.S. common races-ID1, IA1, IB49, IE1, IA45, IB1, IC17, IB45, and IH1-of M. oryzae. An F-2 mapping population consisting of 2,381 individuals derived from RIL171was evaluated with a field isolate (race) ARB82 (IA1) of M. oryzae under greenhouse conditions. Disease reaction of a resistant/susceptible ratio of 3:1 was identified with F-2:F-3 families. In total, 12 simple sequence repeat markers spanning qPi93-3 were used for fine mapping. Consequently, qPi93-3 was delimited to 4.2 Mb between RM3246 and RM7102. Three insertion-deletion (InDel) markers located between RM3246 and RM7102, that had previously used to map Pi61(t), showed that qPi93-3 was Pi61(t). The existence of Pi61(t) in 136 rice germplasm lines from the United States Department of Agriculture rice core collection was evaluated using Pi61(t)-specific InDel markers. Pi61(t) was identified as a source of resistance in 5 of the 136 lines. The characterized germplasm will be useful for rice breeders to use for improving blast resistance. C1 [Ma, Jianbing] Univ Arkansas, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. [Jia, M. H.; Jia, Y.] USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Jia, Y (reprint author), USDA ARS, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. EM yulin.jia@ars.usda.gov FU U.S. National Science Foundation Plant Genome Research Program [0701745] FX The project was supported, in part, by the U.S. National Science Foundation Plant Genome Research Program Number 0701745. We thank M. Lin, T. Bianco, Y. Liu, and A. Jackson for excellent technical assistance and useful discussions. NR 31 TC 2 Z9 3 U1 0 U2 19 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1200 EP 1204 DI 10.1094/PDIS-09-13-1014-RE PG 5 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300004 ER PT J AU Ersahin, YS Weiland, JE Zasada, IA Reed, RL Stevens, JF AF Ersahin, Yurdagul Simsek Weiland, Jerry E. Zasada, Inga A. Reed, Ralph L. Stevens, J. Fred TI Identifying Rates of Meadowfoam (Limnanthes alba) Seed Meal Needed for Suppression of Meloidogyne hapla and Pythium irregulare in Soil SO PLANT DISEASE LA English DT Article ID DEGRADATION-PRODUCTS; MUSTARD; ISOTHIOCYANATES; BIOHERBICIDES; BRASSICAS; PATHOGENS; NITROGEN; PLANTS; WEEDS AB Meadowfoam (Limnanthes alba) is a commercial oilseed annual crop grown in Oregon. After extracting oil from seed, the remaining seed meal is rich in the secondary plant metabolite glucolimnanthin, which can be converted into pesticidal compounds such as 3-methoxybenzyl isothiocyanate (ITC) and 3-methoxyphenylacetonitrile (nitrite) in the presence of the enzyme myrosinase. In previous studies, we demonstrated that ITC and nitrite, produced by mixing freshly ground meadowfoam seed with meadowfoam seed meal, are toxic to the plant-parasitic nematode Meloidogyne hapla and the plant pathogen Pythium irregulare. In this study, we evaluated factors that might influence the implementation of meadowfoam seed meal into agricultural production systems for soilborne pathogen and nematode control. Rate-finding experiments demonstrated that a minimum 1.0% seed/seed meal formulation (wt/wt) was necessary to achieve nematode and pathogen suppression; seed meal alone was insufficient for complete control of M. hapla and stimulated the growth of P. irregulare. When this 1.0% seed/seed meal formulation was used, a greater soil amendment rate was required to cause 100% mortality of P. irregulare (1.0% wt/wt) than for M. hapla (0.5% wt/wt). In phytotoxicity experiments, soil amended with the 1.0% seed/seed meal formulation was consistently phytotoxic to wheat, cucumber, and tomato. However, phytotoxic effects were mitigated by a delayed planting into the amended soil. A final assay to monitor concentrations of ITC and nitrite in conjunction with assessing M. hapla and P irregulare mortality was conducted over a 6-day period in soils amended at 0.5 and 1.0% (wt/wt) with the 1.0% seed/seed meal formulation. The response was rapid, with 100% mortality of both organisms within 2 h after exposure to amended soil. Concentrations of nitrite remained relatively constant over the 6-day period (approximately 0.017 and 0.032 mg/ml at 0.5 and 1.0% amendment rates, respectively), whereas ITC production increased rapidly and peaked 12 to 24 h after amendment (0.083 and 0.171 mg/ml at 0.5 and 1.0% amendment rates, respectively) before returning to near undetectable levels. C1 [Ersahin, Yurdagul Simsek] Cankiri Karatekin Univ, TR-18200 Cankiri, Turkey. [Weiland, Jerry E.; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Reed, Ralph L.; Stevens, J. Fred] Oregon State Univ, Dept Pharmaceut Sci, Corvallis, OR 97331 USA. RP Weiland, JE (reprint author), USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. EM Jerry.Weiland@ars.usda.gov OI Stevens, Jan Frederik/0000-0002-6348-9347 FU Republic of Turkey Council of Higher Education; United States Department of Agriculture [CRIS 303-5358-12220-003-00D] FX Funding for this project was provided by the Republic of Turkey Council of Higher Education and United States Department of Agriculture CRIS 303-5358-12220-003-00D. We thank Natural Plant Products, Inc., Salem, OR for meadowfoam seed and seed meal; and A. Davis, B. Beck, D. Kroese, S. Intanon, and A. Peetz for technical assistance. NR 33 TC 0 Z9 0 U1 0 U2 7 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1253 EP 1260 DI 10.1094/PDIS-09-13-0967-RE PG 8 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300012 ER PT J AU Twizeyimana, M Ojiambo, PS Bandyopadhyay, R Hartman, GL AF Twizeyimana, M. Ojiambo, P. S. Bandyopadhyay, R. Hartman, G. L. TI Use of Quantitative Traits to Assess Aggressiveness of Phakopsora pachyrhizi Isolates from Nigeria and the United States SO PLANT DISEASE LA English DT Article ID SOYBEAN RUST; PATHOGENIC VARIATION; PLANT-PATHOGENS; LEAF RUST; RESISTANCE; VIRULENCE; YIELD; COLONIZATION; EPIDEMIOLOGY; ADAPTATION AB Soybean rust, caused by Phakopsora pachyrhizi, is one of the most important foliar diseases of soybean worldwide. The soybean P. pachyrhizi interaction is often complex due to genetic variability in host and pathogen genotypes. In a compatible reaction, soybean genotypes produce tan-colored lesions, whereas in an incompatible reaction soybean genotypes produce an immune response (complete resistance) or reddish-brown lesions (incomplete resistance). In this study, in total, 116 and 72 isolates of P pachyrhizi from Nigeria and the United States, respectively, were compared based on six quantitative traits to assess their aggressiveness on two soybean genotypes. All isolates produced reddish-brown lesions on plant introduction (PI) 462312 and tan lesions on TGx 1485-1D. The number of days after inoculation to first appearance of lesions, uredinia, and sporulation, along with the number of lesions and sporulating uredinia per square centimeter of leaf tissue, and the number of uredinia per lesion, were significantly (P < 0.001) different between the two soybean genotypes for all isolates from each country. The number of days to first appearance of lesions, uredinia, and sporulation were greater on PI 462312 than on TGx 1485-1D for all the test isolates. Similarly, the number of lesions and sporulating uredinia per square centimeter, and the number of uredinia per lesion were lower on PI 462312 than on TGx 1485-1D. For both soybean genotypes, the number of sporulating uredinia per square centimeter significantly (P = 0.0001) increased with an increase in the number of lesions per square centimeter. Although the slope of the regression of sporulating uredinia on number of lesions was greater (P < 0.0001) when TGx 1485-1D was inoculated with Nigerian isolates compared with U.S. isolates, slopes of the regression lines did not differ significantly (P > 0.0675) when PI 46312 was inoculated with Nigerian or U.S. isolates. This is the first study that used a large number of isolates from two continents to assess aggressiveness of P pachyrhizi using multiple traits in soybean genotypes with contrasting types of disease reaction. C1 [Twizeyimana, M.; Hartman, G. L.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Ojiambo, P. S.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Bandyopadhyay, R.] Int Inst Trop Agr, Ibadan, Nigeria. [Hartman, G. L.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. RP Hartman, GL (reprint author), Univ Illinois, USDA ARS, Urbana, IL 61801 USA. EM ghartman@illinois.edu OI Twizeyimana, Mathias/0000-0003-2173-6693 FU United Soybean Board FX We thank the United Soybean Board for partial financial support. NR 34 TC 0 Z9 0 U1 0 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1261 EP 1266 DI 10.1094/PDIS-12-13-1247-RE PG 6 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300013 ER PT J AU Blomquist, CL Scheck, HJ Woods, PW Bischoff, JF AF Blomquist, C. L. Scheck, H. J. Woods, P. W. Bischoff, J. F. TI First Detection of Puccinia ballotiflora on Salvia greggii. SO PLANT DISEASE LA English DT News Item C1 [Blomquist, C. L.; Woods, P. W.] CDFA, Sacramento, CA 95832 USA. [Scheck, H. J.] Agr Weights & Measures, Santa Barbara, CA 93110 USA. [Bischoff, J. F.] USDA APHIS PPQ NIS, Beltsville, MD 20705 USA. RP Blomquist, CL (reprint author), CDFA, Sacramento, CA 95832 USA. NR 4 TC 0 Z9 0 U1 0 U2 1 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1270 EP 1270 DI 10.1094/PDIS-11-13-1142-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300022 ER PT J AU Caesar, AJ Lartey, RT Caesar-TonThat, T Gaskin, J AF Caesar, A. J. Lartey, R. T. Caesar-TonThat, T. Gaskin, J. TI First Report of Rhizoctonia spp. Causing a Root Rot of the Invasive Rangeland Weed Lepidium draba in North America SO PLANT DISEASE LA English DT News Item C1 [Caesar, A. J.; Lartey, R. T.; Caesar-TonThat, T.; Gaskin, J.] USDA ARS, Northern Plains Agr Res Lab, Sidney, MT 59270 USA. RP Caesar, AJ (reprint author), USDA ARS, Northern Plains Agr Res Lab, 1500 North Cent Ave, Sidney, MT 59270 USA. NR 4 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1278 EP 1279 DI 10.1094/PDIS-03-14-0300-PDN PG 2 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300045 ER PT J AU Klopfenstein, NB Hanna, JW Cannon, PG Medel-Ortiz, R Alvarado-Rosales, D Lorea-Hernandez, F Elias-Roman, RD Kim, MS AF Klopfenstein, N. B. Hanna, J. W. Cannon, P. G. Medel-Ortiz, R. Alvarado-Rosales, D. Lorea-Hernandez, F. Elias-Roman, R. D. Kim, M. -S. TI First Report of the Armillaria Root-Disease Pathogen, Armillaria gallica, Associated with Several Woody Hosts in Three States of Mexico SO PLANT DISEASE LA English DT News Item C1 [Klopfenstein, N. B.; Hanna, J. W.] US Forest Serv, USDA, RMRS, Moscow, ID 83843 USA. [Cannon, P. G.] US Forest Serv, USDA, FHP, Vallejo, CA 94593 USA. [Medel-Ortiz, R.] Univ Veracruzana, Inst Invest Forestales, Xalapa 91070, Veracruz, Mexico. [Alvarado-Rosales, D.] Colegio Postgrad, Programa Fitopatol, Montecillo 56230, Edo De Mexico, Mexico. [Lorea-Hernandez, F.] INECOL, Xalapa 91000, Veracruz, Mexico. [Elias-Roman, R. D.] Fdn Salvador Sanchez Colin CICTAMEX SC, Coatepec Harinas 51700, Estado De Mexic, Mexico. [Kim, M. -S.] Kookmin Univ, Dept Forestry Environm & Syst, Seoul 136702, South Korea. RP Klopfenstein, NB (reprint author), US Forest Serv, USDA, RMRS, Moscow, ID 83843 USA. NR 4 TC 0 Z9 0 U1 2 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1280 EP 1280 DI 10.1094/PDIS-04-14-0349-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300049 ER PT J AU Li, XJ Liu, F Li, YY Zhang, SY Li, MR Li, RH Li, F AF Li, X. J. Liu, F. Li, Y. Y. Zhang, S. Y. Li, M. R. Li, R. H. Li, F. TI First Report of Tomato yellow leaf curl China virus with Betasatellite Infecting Panax notoginseng SO PLANT DISEASE LA English DT News Item C1 [Li, X. J.; Liu, F.; Li, Y. Y.; Zhang, S. Y.; Li, M. R.; Li, F.] Yunnan Agr Univ, Key Lab Agr Biodivers Pest Management, China Educ Minist, Kunming 650201, Peoples R China. [Li, R. H.] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. RP Li, XJ (reprint author), Yunnan Agr Univ, Key Lab Agr Biodivers Pest Management, China Educ Minist, Kunming 650201, Peoples R China. NR 3 TC 0 Z9 3 U1 2 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD SEP PY 2014 VL 98 IS 9 BP 1284 EP 1284 DI 10.1094/PDIS-03-14-0255-PDN PG 1 WC Plant Sciences SC Plant Sciences GA AN8OU UT WOS:000340864300059 ER PT J AU Xu, WP Peng, H Yang, TB Whitaker, B Huang, LH Sun, JH Chen, P AF Xu, Wenping Peng, Hui Yang, Tianbao Whitaker, Bruce Huang, Luhong Sun, Jianghao Chen, Pei TI Effect of calcium on strawberry fruit flavonoid pathway gene expression and anthocyanin accumulation SO PLANT PHYSIOLOGY AND BIOCHEMISTRY LA English DT Article DE Anthocyanins; Calcium; Fruit color; Phenolics; Woodland strawberry ID DIPLOID STRAWBERRY; BIOSYNTHESIS; PLANTS; APPLE AB Two diploid woodland strawberry (Fragaria vesca) inbred lines, Ruegen F7-4 (red fruit-bearing) and YW5AF7 (yellow fruit-bearing) were used to study the regulation of anthocyanin biosynthesis in fruit. Ruegen F7-4 fruit had similar total phenolics and anthocyanin contents to commercial octoploid (F. x ananassa) cultivar Seascape, while YW5AF7 exhibited relatively low total phenolics content and no anthocyanin accumulation. Foliar spray of CaCl2 boosted fruit total phenolics content, especially anthocyanins, by more than 20% in both Seascape and RF7-4. Expression levels of almost all the flavonoid pathway genes were comparable in Ruegen F7-4 and YW5AF7 green-stage fruit. However, at the turning and ripe stages, key anthocyanin structural genes, including flavanone 3-hydroxylase (F3H1), dihydroflavonol 4-reductase (DFR2), anthocyanidin synthase (ANS1), and UDP-glucosyltransferase (UGT1), were highly expressed in Ruegen F7-4 compared with YW5AF7 fruit. Calcium treatment further stimulated the expression of those genes in Ruegen F7-4 fruit. Anthocyanins isolated from petioles of YW5AF7 and Ruegen F-7 had the same HPLC DAD profile, which differed from that of Ruegen F-7 fruit anthocyanins. All the anthocyanin structural genes except FvUGT1 were detected in petioles of YW5AF7 and Ruegen F-7. Taken together, these results indicate that the "yellow" gene in YW5AF7 is a fruit specific regulatory gene(s) for anthocyanin biosynthesis. Calcium can enhance accumulation of anthocyanins and total phenolics in fruit possibly via upregulation of anthocyanin structural genes. Our results also suggest that the anthocyanin biosynthesis machinery in petioles is different from that in fruit. Published by Elsevier Masson SAS. C1 [Xu, Wenping] Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China. [Xu, Wenping; Peng, Hui; Yang, Tianbao; Whitaker, Bruce; Huang, Luhong] USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. [Peng, Hui] Guangxi Normal Univ, Coll Life Sci, Guangxi 541004, Peoples R China. [Huang, Luhong] Hunan Acad Agr Sci, Hunan Agr Prod Proc Inst, Changsha 410125, Hunan, Peoples R China. [Sun, Jianghao; Chen, Pei] USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA. RP Yang, TB (reprint author), USDA ARS, Beltsville Agr Res Ctr, Food Qual Lab, Beltsville, MD 20705 USA. EM tianbao.yang@ars.usda.gov FU USDA-ARS project [1245-43000-012-00D] FX The authors would like to thank Ernie Paroczay for excellent technical support, Janet Slovin for providing the seeds of F. vescia inbred lines, and Gene Lester for helping with total phenolics and anthocyanin determinations. Use of a company or product name by the U.S. Department of Agriculture does not imply approval or recommendation of the product to the exclusion of others that may also be suitable. This research was funded by USDA-ARS project No. 1245-43000-012-00D. NR 30 TC 12 Z9 12 U1 8 U2 72 PU ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER PI PARIS PA 23 RUE LINOIS, 75724 PARIS, FRANCE SN 0981-9428 J9 PLANT PHYSIOL BIOCH JI Plant Physiol. Biochem. PD SEP PY 2014 VL 82 BP 289 EP 298 DI 10.1016/j.plaphy.2014.06.015 PG 10 WC Plant Sciences SC Plant Sciences GA AN6IS UT WOS:000340698800033 PM 25036468 ER PT J AU Sun, JD Feng, ZZ Leakey, ADB Zhu, XG Bernacchi, CJ Ort, DR AF Sun, Jindong Feng, Zhaozhong Leakey, Andrew D. B. Zhu, Xinguang Bernacchi, Carl J. Ort, Donald R. TI Inconsistency of mesophyll conductance estimate causes the inconsistency for the estimates of maximum rate of Rubisco carboxylation among the linear, rectangular and non-rectangular hyperbola biochemical models of leaf photosynthesis-A case study of CO2 enrichment and leaf aging effects in soybean SO PLANT SCIENCE LA English DT Article DE A/C-i curves; Mesophyll conductance; Maximum rates of carboxylation; Soybean; CO2; Leaf age ID GAS-EXCHANGE; ELECTRON-TRANSPORT; ELEVATED CO2; CHLOROPHYLL FLUORESCENCE; ARABIDOPSIS-THALIANA; TEMPERATURE RESPONSE; HIGHER-PLANTS; IN-VIVO; LEAVES; PARAMETERS AB The responses of CO2 assimilation to [CO2] (A/C-i) were investigated at two developmental stages (R5 and R6) and in several soybean cultivars grown under two levels of CO2, the ambient level of 370 mu bar versus the elevated level of 550 mu bar. The A/C-i, data were analyzed and compared by either the combined iterations or the separated iterations of the Rubisco-limited photosynthesis (A(c)) and/or the RuBP-limitedphotosynthesis (A(j)) using various curve-fitting methods: the linear 2-segment model; the non-rectangular hyperbola model; the rectangular hyperbola model; the constant rate of electron transport (J) method and the variable J method. Inconsistency was found among the various methods for the estimation of the maximum rate of carboxylation (V-cmax), the mitochondrial respiration rate in the light (Rd) and mesophyll conductance (g(m)). The analysis showed that the inconsistency was due to inconsistent estimates of g(m) values that decreased with an instantaneous increase in [CO2], and varied with the transition C-i cut-off between Rubisco-limited photosynthesis and RuBP-regeneration-limited photosynthesis, and due to over-parameters for non-linear curve-fitting with g(m), included. We proposed an alternate solution to A/C-i curve-fitting for estimates of V-cmax, Rd, J(max) and g(m) with the various A/C-i, curve-fitting methods. The study indicated that down-regulation of photosynthetic capacity by elevated [CO2] and leaf aging was due to partially the decrease in the maximum rates of carboxylation and partially the decrease in g(m). Mesophyll conductance lowered photosynthetic capacity by 18% on average for the case of soybean plants. (C) 2014 Elsevier Ireland Ltd. All rights reserved. C1 [Sun, Jindong; Feng, Zhaozhong; Leakey, Andrew D. B.; Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Leakey, Andrew D. B.; Zhu, Xinguang; Bernacchi, Carl J.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.; Ort, Donald R.] USDA ARS, Photosynth Res Unit, Urbana, IL 61801 USA. RP Sun, JD (reprint author), DuPont Pioneer, Johnston, IA 50131 USA. EM jindong.sun@pioneer.com RI Leakey, Andrew/Q-9889-2016 OI Leakey, Andrew/0000-0001-6251-024X NR 38 TC 4 Z9 5 U1 3 U2 49 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD SEP PY 2014 VL 226 SI SI BP 49 EP 60 DI 10.1016/j.plantsci.2014.06.015 PG 12 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AO0EX UT WOS:000340983000007 PM 25113450 ER PT J AU Bunce, JA AF Bunce, James A. TI Limitations to soybean photosynthesis at elevated carbon dioxide in free-air enrichment and open top chamber systems SO PLANT SCIENCE LA English DT Article DE Soybean; Photosynthesis; Free-air CO2 enrichment; Open top chamber; CO2; Acclimation ID CO2 ENRICHMENT; ATMOSPHERIC CO2; LONG-TERM; STOMATAL CONDUCTANCE; LEAF PHOTOSYNTHESIS; RISING CO2; TEMPERATURE; ACCLIMATION; PRODUCTIVITY; STIMULATION AB It has been suggested that the stimulation of soybean photosynthesis by elevated CO2 was less in free-air carbon dioxide enrichment (FACE) systems than in open top chambers (OTC), which might explain smaller yield increases at elevated CO2 in FACE systems. However, this has not been tested using the same cultivars grown in the same location. I tested whether soybean photosynthesis at high light and elevated CO2 (ambient + 180 mu mol mol(-1)) was limited by electron transport (J) in FACE systems but by ribulose-bisphosphate carboxylation capacity (V-Cmax) in OTC. FACE systems with daytime and continuous CO2 enrichment were also compared. The results indicated that in both cultivars examined, midday photosynthesis at high light was always limited by V-Cmax, both in the FACE and in the OTC systems. Daytime only CO2 enrichment did not affect photosynthetic parameters or limitations, but did result in significantly smaller yields in both cultivars than continuous elevation. Photosynthesis measured at low photosynthetic photon flux density (PPFD) was not higher at elevated than at ambient CO2, because of an acclimation to elevated CO2 which was only evident at low measurement PPFDs. Published by Elsevier Ireland Ltd. C1 USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. RP Bunce, JA (reprint author), USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. EM james.Bunce@ars.usda.gov NR 27 TC 6 Z9 6 U1 3 U2 45 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD SEP PY 2014 VL 226 SI SI BP 131 EP 135 DI 10.1016/j.plantsci.2014.01.002 PG 5 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AO0EX UT WOS:000340983000015 PM 25113458 ER PT J AU Rosenthal, DM Ruiz-Vera, UM Siebers, MH Gray, SB Bernacchi, CJ Ort, DR AF Rosenthal, David M. Ruiz-Vera, Ursula M. Siebers, Matthew H. Gray, Sharon B. Bernacchi, Carl J. Ort, Donald R. TI Biochemical acclimation, stomatal limitation and precipitation patterns underlie decreases in photosynthetic stimulation of soybean (Glycine max) at elevated [CO2] and temperatures under fully open air field conditions SO PLANT SCIENCE LA English DT Article DE Carboxylation; RuBP regeneration; Stomatal limitation; Photosynthetic nitrogen use efficiency; V-c,V-max; J(max) ID MARIANA MILL. BSP; LONG-TERM CO2; CARBON-DIOXIDE; IN-VIVO; GROWTH TEMPERATURE; ATMOSPHERIC CO2; C-3 PHOTOSYNTHESIS; ENRICHMENT FACE; INFRARED HEATER; RISING CO2 AB The net effect of elevated [CO2] and temperature on photosynthetic acclimation and plant productivity is poorly resolved. We assessed the effects of canopy warming and fully open air [CO2] enrichment on (1) the acclimation of two biochemical parameters that frequently limit photosynthesis (A), the maximum carboxylation capacity of Rubisco (V-c,V-max) and the maximum potential linear electron flux through photo-system II (J(max)), (2) the associated responses of leaf structural and chemical properties related to A, as well as (3) the stomatal limitation (l) imposed on A, for soybean over two growing seasons in a conventionally managed agricultural field in Illinois, USA. Acclimation to elevated [CO2] was consistent over two growing seasons with respect to V-c,V-max and J(max). However, elevated temperature significantly decreased J(max) contributing to lower photosynthetic stimulation by elevated CO2. Large seasonal differences in precipitation altered soil moisture availability modulating the complex effects of elevated temperature and CO2 on biochemical and structural properties related to A. Elevated temperature also reduced the benefit of elevated [CO2] by eliminating decreases in stomatal limitation at elevated [CO2]. These results highlight the critical importance of considering multiple environmental factors (i.e. temperature, moisture, [CO2]) when trying to predict plant productivity in the context of climate change. (C) 2014 Elsevier Ireland Ltd. All rights reserved. C1 [Rosenthal, David M.] Ohio Univ, Dept Environm & Plant Biol, Athens, OH 45701 USA. [Ruiz-Vera, Ursula M.; Siebers, Matthew H.; Gray, Sharon B.; Bernacchi, Carl J.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Bernacchi, Carl J.; Ort, Donald R.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Bernacchi, Carl J.; Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. RP Ort, DR (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM rosentha@ohio.edu; d-ort@illinois.edu OI Rosenthal, David M/0000-0002-4822-5861 FU United States Department of Agriculture (USDA) Agriculture Research Service (ARS); Office of Science (BER), US Department of Energy, through the Midwestern Center of the National Institute for Climate Change Research (NICCR) FX We gratefully acknowledge the dedication of David Drag to ensuring the successful operation of T-FACE. We also acknowledge both Kannan Puthuval and David Drag for managing the overall SoyFACE site. We thank John Spiekerman, Stephanie Klein, Natalia Ordonez, and Becky Slattery for helping with gas exchange measurements. Funding for this research was provided by the United States Department of Agriculture (USDA) Agriculture Research Service (ARS) and by the Office of Science (BER), US Department of Energy, through the Midwestern Center of the National Institute for Climate Change Research (NICCR). NR 65 TC 6 Z9 7 U1 6 U2 59 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD SEP PY 2014 VL 226 SI SI BP 136 EP 146 DI 10.1016/j.plantsci.2014.06.013 PG 11 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AO0EX UT WOS:000340983000016 PM 25113459 ER PT J AU Sun, JD Feng, ZZ Ort, DR AF Sun, Jindong Feng, Zhaozhong Ort, Donald R. TI Impacts of rising tropospheric ozone on photosynthesis and metabolite levels on field grown soybean SO PLANT SCIENCE LA English DT Article DE Ozone; Soybean; Crop yield; Photosynthesis; Metabolites ID RUBISCO-LIMITED PHOTOSYNTHESIS; BETULA-PENDULA ROTH; MESOPHYLL CONDUCTANCE; ELEVATED CO2; CHLOROPHYLL FLUORESCENCE; ANTIOXIDATIVE DEFENSE; TEMPERATURE RESPONSE; AGRICULTURAL CROPS; CARBON METABOLISM; FAGUS-SYLVATICA AB The response of leaf photosynthesis and metabolite profiles to ozone (O-3) exposure ranging from 37 to 116 ppb was investigated in two soybean cultivars Dwight and IA3010 in the field under fully open-air conditions. Leaf photosynthesis, total non-structural carbohydrates (TNC) and total free amino acids (TAA) decreased linearly with increasing O-3 levels in both cultivars with average decrease of 7% for an increase in O-3 levels by 10 ppb. Ozone interacted with developmental stages and leaf ages, and caused higher damage at later reproductive stages and in older leaves. Ozone affected yield mainly via reduction of maximum rate of Rubisco carboxylation (V-cmax) and maximum rates of electron transport (J(max)) as well as a shorter growing season due to earlier onset of canopy senescence. For all parameters investigated the critical O-3 levels (similar to 50 ppb) for detectable damage fell within O-3 levels that occur routinely in soybean fields across the US and elsewhere in the world. Strong correlations were observed in O-3-induced changes among yield, photosynthesis, TNC, TAA and many metabolites. The broad range of metabolites that showed O-3 dose dependent effect is consistent with multiple interaction loci and thus multiple targets for improving the tolerance of soybean to O-3. (C) 2014 Elsevier Ireland Ltd. All rights reserved. C1 [Sun, Jindong; Feng, Zhaozhong; Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. RP Sun, JD (reprint author), DuPont Pioneer, Johnston, IA 50131 USA. EM jindong.sun@gmail.com NR 59 TC 9 Z9 11 U1 1 U2 41 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0168-9452 J9 PLANT SCI JI Plant Sci. PD SEP PY 2014 VL 226 SI SI BP 147 EP 161 DI 10.1016/j.plantsci.2014.06.012 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AO0EX UT WOS:000340983000017 PM 25113460 ER PT J AU Huyler, A Chappelka, AH Prior, SA Somers, GL AF Huyler, Ann Chappelka, Arthur H. Prior, Stephen A. Somers, Greg L. TI Influence of aboveground tree biomass, home age, and yard maintenance on soil carbon levels in residential yards SO URBAN ECOSYSTEMS LA English DT Article DE Soil carbon; Residential yards; Aboveground tree biomass; Home age; Yard maintenance ID ATMOSPHERIC CO2 ENRICHMENT; BELOW-GROUND BIOMASS; SCOTS PINE FOREST; ORGANIC-CARBON; UNITED-STATES; TERRESTRIAL ECOSYSTEMS; EUCALYPTUS-GLOBULUS; NITROGEN DYNAMICS; DECIDUOUS FOREST; ROOT DYNAMICS AB With the rapid urbanization of natural lands, researchers have begun to examine the capacity of urban soils to store carbon (C), with recent attention to residential yards. We performed a case study to examine four potential influences on soil C levels in residential yards. In 67 yards containing trees, we examined the relationship of soil C (kg m(-2)) to tree aboveground biomass, home age (3-87 years), yard maintenance (fertilization, irrigation, mulching or bagging lawn clippings), and soil texture (% clay, % sand, % silt), at three depths (0-15 cm, 15-30 cm, and 30-50 cm). Six tree aboveground biomass data sets were developed: 1) biomass, 2) biomass*(1/distance from tree), 3) biomass a parts per thousand currency signaEuro parts per thousand 15 m from sample site, 4) biomass a parts per thousand currency signaEuro parts per thousand 10 m, 5) biomass a parts per thousand currency signaEuro parts per thousand 5 m, and 6) biomass a parts per thousand currency signaEuro parts per thousand 4 m. Biomass a parts per thousand currency signaEuro parts per thousand 5 m and biomass a parts per thousand currency signaEuro parts per thousand 4 m had the greatest explanatory power for soil C at 30-50 cm depth (P = 0.001, R-2 = 0.28; P = 0.05 R-2 = 0.39, respectively). The relationship between soil C and home age was positive at 0-15 cm (P = 0.0003, R-2 = 0.19), but constant at the two lower depths. Yard maintenance had no significant influence on soil C levels across home age. At 0-15 cm, soil C increased with % silt (P = 0.006, R-2 = 0.12). Overall, trees in turfgrass yards may have a stabilizing effect on soil C levels below 15 cm but minimal influence above 15 cm. C1 [Huyler, Ann; Chappelka, Arthur H.; Somers, Greg L.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Prior, Stephen A.] ARS, USDA, Natl Soil Dynam Lab, Auburn, AL 36832 USA. RP Huyler, A (reprint author), Auburn Univ, Sch Forestry & Wildlife Sci, 602 Duncan Dr, Auburn, AL 36849 USA. EM agh0001@tigermail.auburn.edu; chappah@auburn.edu; steve.prior@ars.usda.gov; somergl@auburn.edu FU Mcintire-Stennis funds FX We are very thankful for the technical and/or field assistance from William Avery, Jonathon Bartlett, Kyle Bolton, Nick Gilliland, Robin Governo, Dr. B. Graeme Lockaby, Nick Martin, Matthew McCollough and Efrem Robbins. For carbon and nitrogen analysis and technical assistance, we are greatly appreciative of Barry G. Dorman and Dr. Juan B. Rodriguez with the National Soil Dynamics Laboratory, Auburn, AL. In addition, we are very grateful for the thorough, accurate editorial contributions from Dr. Francisco Escobedo and the 2 anonymous reviewers. Partial financial support provided by Mcintire-Stennis funds. NR 75 TC 3 Z9 3 U1 1 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD SEP PY 2014 VL 17 IS 3 BP 787 EP 805 DI 10.1007/s11252-014-0350-7 PG 19 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA AO1RG UT WOS:000341090700011 ER PT J AU Falxa-Raymond, N Palmer, MI McPhearson, T Griffin, KL AF Falxa-Raymond, Nancy Palmer, Matthew I. McPhearson, Timon Griffin, Kevin L. TI Foliar nitrogen characteristics of four tree species planted in New York City forest restoration sites SO URBAN ECOSYSTEMS LA English DT Article DE Amelanchier canadensis; Forest restoration; Nitrate reductase; Nyssa sylvatica; Prunus serotina; Quercus rubra; Stable isotopes ID NITRATE REDUCTASE-ACTIVITY; SOUTHEASTERN NEW-YORK; CONTRASTING ANTHROPOGENIC IMPACTS; URBAN-RURAL GRADIENT; LAND-USE GRADIENT; MYCORRHIZAL FUNGI; NATURAL-ABUNDANCE; N-15 ABUNDANCE; SOIL-NITROGEN; DECIDUOUS FOREST AB Urban forests provide important environmental benefits, leading many municipal governments to initiate citywide tree plantings. However, nutrient cycling in urban ecosystems is difficult to predict, and nitrogen (N) use in urban trees may be quite different from use in rural forests. To gain insight into these biogeochemical and physiological processes, we compared foliar N characteristics of several common northeastern deciduous tree species across four newly planted New York City afforestation sites as well as at the Black Rock Forest (BRF), a rural oak-dominated forest in the Hudson Highlands, New York. Foliage sampled at BRF was consistently depleted in N-15 compared to urban foliage, and Amelanchier canadensis, Nyssa sylvatica, Prunus serotina, and Quercus rubra showed significant variation in foliar nitrogen isotope signatures (delta N-15) among the four urban sites. A. canadensis and P. serotina showed significantly greater ability to assimilate nitrate at BRF compared to urban sites, as measured through nitrate reductase activity (NRA). There were no significant differences in NRA among tree species growing at the four urban sites. Only P. serotina and N. sylvatica showed significant variation in foliar N concentrations (%N) both among urban sites and compared to BRF. The isotopic and %N data suggest greater N availability but less available nitrate at the newly planted urban sites compared to BRF, possibly due to different anthropogenic inputs or higher rates of nitrification and nitrate leaching at the recently planted urban sites compared to likely lower rates of N cycling in the intact rural forest. In addition, the tree species varied in their response to N availability at the urban sites, with potential implications for growth and survival. Understanding N cycling in urban systems and the associated physiological changes in vegetation is critical to a comprehensive evaluation of urban forest restoration, and may have implications for carbon sequestration and water quality issues associated with nitrate export, two important areas of management concern. C1 [Falxa-Raymond, Nancy] US Forest Serv, USDA, No Res Stn, NYC Urban Field Stn, Bayside, NY 11359 USA. [Falxa-Raymond, Nancy; Palmer, Matthew I.; Griffin, Kevin L.] Columbia Univ, Dept Ecol Evolut & Environm Biol, New York, NY 10027 USA. [McPhearson, Timon] New Sch, Tishman Environm & Design Ctr, New York, NY 10003 USA. [Griffin, Kevin L.] Columbia Univ, Dept Earth & Environm Sci, Lamont Doherty Earth Observ, Palisades, NY 10964 USA. RP Falxa-Raymond, N (reprint author), US Forest Serv, USDA, No Res Stn, NYC Urban Field Stn, Ft Totten Cluster 2,Box 12, Bayside, NY 11359 USA. EM njfalxaraymond@fs.fed.us RI Griffin, Kevin/B-2629-2013; OI Griffin, Kevin/0000-0003-4124-3757; McPhearson, Timon/0000-0002-9499-0791 FU Black Rock Forest Consortium small grants program; Garden Club of America Zone VI Fellowship; U.S. National Science Foundation [DEB-0949387] FX This research was funded by The Black Rock Forest Consortium small grants program; The Garden Club of America Zone VI Fellowship; and the U.S. National Science Foundation (DEB-0949387). We thank Bill Schuster, Matthew Turnbull, Novem Auyeung, and Rich Hallett for their advice and support, as well as John Stanovick for his assistance with statistical models. We would also like to thank three anonymous reviewers for their constructive comments. NR 72 TC 1 Z9 1 U1 4 U2 50 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1083-8155 EI 1573-1642 J9 URBAN ECOSYST JI Urban Ecosyst. PD SEP PY 2014 VL 17 IS 3 BP 807 EP 824 DI 10.1007/s11252-014-0346-3 PG 18 WC Biodiversity Conservation; Ecology; Environmental Sciences; Urban Studies SC Biodiversity & Conservation; Environmental Sciences & Ecology; Urban Studies GA AO1RG UT WOS:000341090700012 ER PT J AU Swirski, AL Pearl, DL Williams, ML Homan, HJ Linz, GM Cernicchiaro, N LeJeune, JT AF Swirski, A. L. Pearl, D. L. Williams, M. L. Homan, H. J. Linz, G. M. Cernicchiaro, N. LeJeune, J. T. TI Spatial Epidemiology of Escherichia coli O157:H7 in Dairy Cattle in Relation to Night Roosts Of Sturnus vulgaris (European Starling) in Ohio, USA (2007-2009) SO ZOONOSES AND PUBLIC HEALTH LA English DT Article ID HEMOLYTIC-UREMIC-SYNDROME; O157-H7 INFECTIONS; FOODBORNE ILLNESS; OUTBREAK; PREVALENCE; WASHINGTON; PATHOGENS; FEEDLOT; DISEASE; CANADA AB The goal of our study was to use spatial scan statics to determine whether the night roosts of European starlings (Sturnus vulgaris) act as point sources for the dissemination of Escherichia coli O157:H7 among dairy farms. From 2007 to 2009, we collected bovine faecal samples (n = 9000) and starling gastrointestinal contents (n = 430) from 150 dairy farms in northeastern Ohio, USA. Isolates of E. coli O157:H7 recovered from these samples were subtyped using multilocus variable-number tandem repeat analysis (MLVA). Generated MLVA types were used to construct a dendrogram based on a categorical multistate coefficient and unweighted pair-group method with arithmetic mean (UPGMA). Using a focused spatial scan statistic, we identified statistically significant spatial clusters among dairy farms surrounding starling night roosts, with an increased prevalence of E. coli O157:H7-positive bovine faecal pats, increased diversity of distinguishable MLVA types and a greater number of isolates with MLVA types from bovine-starling clades versus bovine-only clades. Thus, our findings are compatible with the hypothesis that starlings have a role in the dissemination of E. coli O157:H7 among dairy farms, and further research into starling management is warranted. C1 [Swirski, A. L.; Pearl, D. L.] Univ Guelph, Ontario Vet Coll, Dept Populat Med, Guelph, ON N1G 2W1, Canada. [Williams, M. L.; LeJeune, J. T.] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food & Anim Hlth Res Program, Wooster, OH 44691 USA. [Homan, H. J.; Linz, G. M.] Wildlife Serv, USDA, Natl Wildlife Res Ctr, Bismarck, ND USA. [Cernicchiaro, N.] Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA. RP Swirski, AL (reprint author), Univ Guelph, Ontario Vet Coll, Dept Populat Med, Guelph, ON N1G 2W1, Canada. EM aswirski@uoguelph.ca; dpearl@uoguelph.ca FU Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture, Epidemiological Approaches to Food Safety Grant [2006-01227]; Canada Foundation for Innovation; Ontario Research Fund FX This research was supported by the Agriculture and Food Research Initiative of the USDA National Institute of Food and Agriculture, Epidemiological Approaches to Food Safety Grant #2006-01227 and the infrastructure grant to D. L. Pearl from the Canada Foundation for Innovation and the Ontario Research Fund. NR 31 TC 6 Z9 6 U1 2 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1863-1959 EI 1863-2378 J9 ZOONOSES PUBLIC HLTH JI Zoonoses Public Health PD SEP PY 2014 VL 61 IS 6 BP 427 EP 435 DI 10.1111/zph.12092 PG 9 WC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences SC Public, Environmental & Occupational Health; Infectious Diseases; Veterinary Sciences GA AN5VO UT WOS:000340659900007 PM 24279810 ER PT J AU Skoracka, A Rector, B Kuczynski, L Szydlo, W Hein, G French, R AF Skoracka, A. Rector, B. Kuczynski, L. Szydlo, W. Hein, G. French, R. TI Global spread of wheat curl mite by its most polyphagous and pestiferous lineages SO ANNALS OF APPLIED BIOLOGY LA English DT Article DE Aceria tosichella; invasiveness; cryptic species; Eriophyidae; host range; molecular taxonomy ID ACERIA-TOSICHELLA KEIFER; TRITICUM-MOSAIC-VIRUS; MAXIMUM-LIKELIHOOD; ERIOPHYOID MITES; NUCLEOTIDE-SEQUENCES; ACARI ERIOPHYOIDEA; DNA; SPECIALIZATION; IDENTIFICATION; MITOCHONDRIAL AB The wheat curl mite (WCM), Aceria tosichella, is an important pest of wheat and other cereal crops that transmits wheat streak mosaic virus and several other plant viruses. Wheat curl mite has long been considered a single polyphagous species, but recent studies in Poland revealed a complex of genetically distinct lineages with divergent host-acceptance traits, ranging from highly polyphagous to host-specific. This diversity of WCM genotypes and host-acceptance phenotypes in Europe, the presumed native range of WCM, raises questions about the lineage identities of invasive WCM populations on other continents and their relationships to European lineages. The goals of this study were to examine the global presence of WCM and determine the relatedness of lineages established in different continents, on the basis of phylogenetic analyses of mitochondrial and nuclear DNA sequence data. Host-range bioassays of a highly polyphagous WCM lineage were performed to supplement existing data on this lineage's ability to colonise graminaceous and non-graminaceous hosts. Invasive WCM populations in North and South America and Australia assorted with the only three known polyphagous and pestiferous WCM lineages ('MT-1', 'MT-7' and 'MT-8') from a total of eight currently described lineages. These results show that the most polyphagous lineages were more successful colonisers and reflect a need for extensive surveys for WCM on both crops and wild grass species in invaded continents. The most invasive lineage ('MT-1') was shown to successfully colonise all 10 plant species tested in three families and has spread to North and South America and Australia from its presumed origins in Eurasia. C1 [Skoracka, A.; Szydlo, W.] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, PL-61614 Poznan, Poland. [Rector, B.] USDA ARS, Great Basin Rangelands Res Unit, Reno, NV USA. [Kuczynski, L.] Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Avian Biol & Ecol, PL-61614 Poznan, Poland. [Hein, G.] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA. [French, R.] Univ Nebraska, USDA ARS, Lincoln, NE 68583 USA. [French, R.] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA. RP Skoracka, A (reprint author), Adam Mickiewicz Univ, Fac Biol, Inst Environm Biol, Dept Anim Taxon & Ecol, Umultowska 89, PL-61614 Poznan, Poland. EM anna.skoracka@amu.edu.pl RI Kuczynski, Lechoslaw/E-5525-2011; Skoracka, Anna/E-6002-2011; Szydlo, Wiktoria/L-4804-2016 OI Kuczynski, Lechoslaw/0000-0003-3498-5445; Skoracka, Anna/0000-0002-9485-532X; Szydlo, Wiktoria/0000-0001-5990-5611 FU National Science Centre in Poland [2011/03/B/NZ8/00129, 2011/01/N/NZ8/04540]; USDA NC-IPM (CSREES) [2004-34103-14443]; USDA-ARS [58-5440-7-334] FX We thank Zofia Banaszak (director of plant breeding centre DANKO) for permission to collect wheat, Miroslawa Dabert (Molecular Biology Techniques Laboratory, Faculty of Biology, Adam Mickiewicz University [AMU]) for designing the primer 28Srev430, Urszula Sobczynska (AMU, Poland) for help with laboratory work, Evsel Denizhan for providing three samples, the editor of Annals of Applied Biology and two anonymous reviewers for helpful comments that greatly improved the manuscript. The study was funded by the National Science Centre in Poland (Grants Nos. 2011/03/B/NZ8/00129 and 2011/01/N/NZ8/04540). The US portion of this project was partially funded by USDA NC-IPM (CSREES) Grant No. 2004-34103-14443 and by USDA-ARS Specific Cooperative Agreement no. 58-5440-7-334. A. S. and L. K. conceived and designed the study. A. S. performed the experiments. A. S., W. S., G. H. and R. F. carried out the molecular genetic studies. L. K., A. S. and W. S. analysed the data. B. R. and A. S. wrote the manuscript. L. K., W. S., G. H. and R. F. partially wrote methods and results. All authors critically revised the manuscript and read and approved the final manuscript. NR 54 TC 8 Z9 8 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0003-4746 EI 1744-7348 J9 ANN APPL BIOL JI Ann. Appl. Biol. PD SEP PY 2014 VL 165 IS 2 BP 222 EP 235 DI 10.1111/aab.12130 PG 14 WC Agriculture, Multidisciplinary SC Agriculture GA AN3ZQ UT WOS:000340527800004 ER PT J AU Zhu, JY Ze, SZ Stanley, DW Yang, B AF Zhu, Jia-Ying Ze, Sang-Zi Stanley, David W. Yang, Bin TI PARASITIZATION BY SCLERODERMA GUANI INFLUENCES EXPRESSION OF SUPEROXIDE DISMUTASE GENES IN TENEBRIO MOLITOR SO ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY LA English DT Article DE superoxide dismutase; parasitoid; immunity; mRNA transcription ID PUPARUM HYMENOPTERA PTEROMALIDAE; DROSOPHILA-MELANOGASTER; MOLECULAR CHARACTERIZATION; BEMISIA-TABACI; IMMUNITY; CLONING; INSECTS; VENOM; RESISTANCE; HEMOCYTES AB Superoxide dismutase (SOD) is an antioxidant enzyme involved in detoxifying reactive oxygen species. In this study, we identified genes encoding the extracellular and intracellular copper-zinc SODs (ecCuZnSOD and icCuZnSOD) and a manganese SOD (MnSOD) in the yellow mealworm beetle, Tenebrio molitor. The cDNAs for ecCuZnSOD, icCuZnSOD, and MnSOD, respectively, encode 24.55, 15.81, and 23.14 kDa polypeptides, which possess structural features typical of other insect SODs. They showed 20-94% identity to other known SOD sequences from Bombyx mori, Musca domestica, Nasonia vitripennis, Pediculus humanus corporis, and Tribolium castaneum. Expression of these genes was analyzed in selected tissues and developmental stages, and following exposure to Escherichia coli and parasitization by Scleroderma guani. We recorded expression of all three SODs in cuticle, fat body, and hemocytes and in the major developmental stages. Relatively higher expressions were detected in late-instar larvae and pupae, compared to other developmental stages. Transcriptional levels were upregulated following bacterial infection. Analysis of pupae parasitized by S. guani revealed that expression of T. molitor SOD genes was significantly induced following parasitization. We infer that these genes act in immune response and in host-parasitoid interactions. (C) 2014 Wiley Periodicals, Inc. C1 [Zhu, Jia-Ying; Yang, Bin] Southwest Forestry Univ, Key Lab Forest Disaster Warning & Control Yunnan, Kunming 650224, Peoples R China. [Ze, Sang-Zi] Yunnan Forestry Technol Coll, Kunming, Peoples R China. [Stanley, David W.] USDA ARS, Biol Control Insects Res Lab, Columbia, MO 65205 USA. RP Zhu, JY (reprint author), Southwest Forestry Univ, Key Lab Forest Disaster Warning & Control Yunnan, Kunming 650224, Peoples R China. EM jyzhu001@gmail.com FU National Natural Science Foundation of China [31260449]; Fund of Reserve Talents for Young and Middle-Aged Leaders of Yunnan Province [2013HB077] FX Grant sponsor: National Natural Science Foundation of China; Grant number: 31260449; Grant sponsor: Fund of Reserve Talents for Young and Middle-Aged Leaders of Yunnan Province; Grant number: 2013HB077. Correspondence to: Jia-Ying Zhu, Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Southwest Forestry University, Kunming 650224, China. E-mail: jyzhu001@gmail.com NR 44 TC 3 Z9 5 U1 5 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0739-4462 EI 1520-6327 J9 ARCH INSECT BIOCHEM JI Arch. Insect Biochem. Physiol. PD SEP PY 2014 VL 87 IS 1 BP 40 EP 52 DI 10.1002/arch.21179 PG 13 WC Biochemistry & Molecular Biology; Entomology; Physiology SC Biochemistry & Molecular Biology; Entomology; Physiology GA AN5CW UT WOS:000340608000004 PM 25042129 ER PT J AU Vivanco, J Jakes, JE Slane, J Ploeg, HL AF Vivanco, Juan Jakes, Joseph E. Slane, Josh Ploeg, Heidi-Lynn TI Accounting for structural compliance in nanoindentation measurements of bioceramic bone scaffolds SO CERAMICS INTERNATIONAL LA English DT Article DE Bioceramic; Bone scaffold; Nanoindentation; Musculoskeletal injuries ID ELASTIC-MODULUS; TRABECULAR BONE; INDENTATION; TISSUE; HARDNESS; BIOMATERIALS; SIZE AB Structural properties have been shown to be critical in the osteoconductive capacity and strength of bioactive ceramic bone scaffolds. Given the cellular foam-like structure of bone scaffolds, nanoindentation has been used as a technique to assess the mechanical properties of individual components of the scaffolds. Nevertheless, nanoindents placed on scaffolds may violate the rigid support assumption of the standard Oliver-Pharr method currently used in evaluating the Meyer hardness, H, and elastic modulus, E-s, of such structures. Thus, the objective of this research was to use the structural compliance method to assess whether or not specimen-scale flexing may occur during nanoindentation of bioceramic bone scaffolds and to remove the associated artifact on the H and E-s if it did occur. Scaffolds were fabricated using tricalcium phosphate and sintered at 950 degrees C and 1150 degrees C, and nanoindents were placed in three different (center, edge, and corner) scaffold locations. Using only the standard Oliver-Pharr analysis it was found that H and E-s were significantly affected by both sintering temperature and nanoindents location (p < 0.05). However, specimen-scale flexing occurred during nanoindentation in the 1150 degrees C corner location. After removing the effects of the flexing from the measurement using the structural compliance method, it was concluded that H and E-s were affected only by the sintering temperature (p < 0.05) irrespective of the nanoindent locations. These results show that specimen-scale flexing may occur during nanoindentation of components in porous bioceramic scaffolds or in similar structure biomaterials, and that the structural compliance method must be utilized to accurately assess H and E-s of these components. (C) 2014 Elsevier Ltd and Techna Group S.r.l. All rights reserved. C1 [Vivanco, Juan; Slane, Josh; Ploeg, Heidi-Lynn] Univ Wisconsin, Dept Mech Engn, Madison, WI 53706 USA. [Jakes, Joseph E.] USDA, Forest Serv, Forest Prod Lab, Forest Biopolymer Sci & Engn, Madison, WI 53726 USA. [Vivanco, Juan] Univ Adolfo Ibanez, Fac Ingn & Ciencias, Vina Del Mar, Chile. [Slane, Josh] Univ Wisconsin, Mat Sci Program, Madison, WI 53706 USA. RP Vivanco, J (reprint author), Univ Wisconsin, Dept Mech Engn, 1513 Univ Ave,Room 2258, Madison, WI 53706 USA. EM vivanco@wisc.edu FU USDA Forest Service PECASE FX The authors wish to thank Humberto Melgarejo for his contribution in sample preparation, Philips Plastic Corporation for providing the scaffolds and Hysitron Inc. for technical support during nano mechanical testing. JEJ acknowledges USDA Forest Service PECASE funding. NR 31 TC 2 Z9 2 U1 1 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0272-8842 EI 1873-3956 J9 CERAM INT JI Ceram. Int. PD SEP PY 2014 VL 40 IS 8 BP 12485 EP 12492 DI 10.1016/j.ceramint.2014.04.103 PN A PG 8 WC Materials Science, Ceramics SC Materials Science GA AN1FR UT WOS:000340328600122 ER PT J AU Williams, JL Iamartino, D Sonstegard, T Reecy, J Van Tassell, C Zimin, A AF Williams, J. L. Iamartino, D. Sonstegard, T. Reecy, J. Van Tassell, C. Zimin, A. CA Int Buffalo Consortium TI The Buffalo Genome: Structure and Function SO CHROMOSOME RESEARCH LA English DT Meeting Abstract CT 21st International Colloquium on Animal Cytogenetics and Gene Mapping (ICACGM) CY JUN 07-10, 2014 CL Naples, ITALY C1 [Williams, J. L.; Iamartino, D.] Parco Tecnol Padano, I-26900 Lodi, Italy. [Sonstegard, T.; Van Tassell, C.] ARS, USDA, Beltsville, MD 20705 USA. [Reecy, J.] Iowa State Univ, Ames, IA USA. [Zimin, A.] Univ Maryland, Inst Phys Sci & Technol, College Pk, MD 20742 USA. EM John.Williams@Tecnoparco.org NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0967-3849 EI 1573-6849 J9 CHROMOSOME RES JI Chromosome Res. PD SEP PY 2014 VL 22 IS 3 MA L9 BP 406 EP 406 PG 1 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA AN4CH UT WOS:000340534700038 ER PT J AU Kapetanakos, YA Lovette, IJ Katzner, TE AF Kapetanakos, Y. A. Lovette, I. J. Katzner, T. E. TI Development of microsatellite markers and a restriction endonuclease digest assay for non-invasive sampling of endangered White-rumped, Slender-billed and Red-headed vultures SO CONSERVATION GENETICS RESOURCES LA English DT Article DE Gyps bengalensis; Gyps tenuirostris; Sarcogyps calvus; Microsatellite; Cambodia ID DECLINES; ASIA AB Southeast Asian vultures have been greatly reduced in range and population numbers, but it is challenging to use traditional tagging and monitoring techniques to track changes in their populations. Genotypes derived from non-invasively collected feather samples provide an alternative and effective means to 'capture' individual vultures for mark-recapture analyses. We describe a restriction endonuclease digest assay that distinguishes the visually similar feathers of three species of critically endangered Asian vultures (Gyps bengalensis, G. tenuirostris, and Sarcogyps calvus). In addition, we describe a panel of eight polymorphic microsatellite loci. In combination, the restriction endonuclease assay and microsatellite marker set developed here are powerful molecular tools for investigating the genetic and demographic status of these Asian vultures species. C1 [Kapetanakos, Y. A.; Lovette, I. J.] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY USA. [Kapetanakos, Y. A.; Lovette, I. J.] Cornell Lab Ornithol, Fuller Evolutionary Biol Program, Ithaca, NY 14850 USA. [Katzner, T. E.] W Virginia Univ, Div Forestry & Nat Resources, Morgantown, WV 26506 USA. [Katzner, T. E.] US Forest Serv, USDA, No Res Stn, Parsons, WV 26287 USA. RP Kapetanakos, YA (reprint author), Cornell Lab Ornithol, Fuller Evolutionary Biol Program, 159 Sapsucker Woods Rd, Ithaca, NY 14850 USA. EM yak4@cornell.edu; ijl2@cornell.edu; Todd.Katzner@mail.wvu.edu RI Lovette, Irby/P-3477-2014; OI Katzner, Todd/0000-0003-4503-8435 FU Association of Zoos and Aquariums; Disney Wildlife Conservation Fund; Riverbanks Zoo; American Museum of Natural History; American Ornithological Union; Eppley Foundation; Cornell Lab of Ornitholo FX We thank the Wildlife Conservation Society (WCS) for access to vulture sites, to WCS, the Cheyenne Mountain Zoo, Birmingham Zoo, and Department of Veterinary Pathology at Kasetsart University for blood and feather samples. This research was supported in part by the Association of Zoos and Aquariums and the Disney Wildlife Conservation Fund, Riverbanks Zoo, American Museum of Natural History, American Ornithological Union, Eppley Foundation, and Cornell Lab of Ornitholo. NR 18 TC 0 Z9 0 U1 1 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1877-7252 EI 1877-7260 J9 CONSERV GENET RESOUR JI Conserv. Genet. Resour. PD SEP PY 2014 VL 6 IS 3 BP 539 EP 542 DI 10.1007/s12686-014-0186-8 PG 4 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA AN2HJ UT WOS:000340404900011 ER PT J AU Jachowski, DS Dobony, CA Coleman, LS Ford, WM Britzke, ER Rodrigue, JL AF Jachowski, David S. Dobony, Chris A. Coleman, Laci S. Ford, William M. Britzke, Eric R. Rodrigue, Jane L. TI Disease and community structure: white-nose syndrome alters spatial and temporal niche partitioning in sympatric bat species SO DIVERSITY AND DISTRIBUTIONS LA English DT Article DE Community structure; disease; Myotis; niche partitioning; spatial niche partitioning; temporal niche partitioning; white-nose syndrome ID ENDANGERED INDIANA BAT; INTERSPECIFIC COMPETITION; INSECTIVOROUS BATS; HABITAT USE; MYOTIS-LUCIFUGUS; SOCIAL CALLS; PIPISTRELLUS-PIPISTRELLUS; LASIURUS-CINEREUS; FIELD EXPERIMENTS; EPTESICUS-FUSCUS AB Aim Emerging infectious diseases present a major perturbation with apparent direct effects such as reduced population density, extirpation and/or extinction. Comparatively less is known about the potential indirect effects of disease that likely alter community structure and larger ecosystem function. Since 2006, white-nose syndrome (WNS) has resulted in the loss of over 6 million hibernating bats in eastern North America. Considerable evidence exists concerning niche partitioning in sympatric bat species in this region, and the unprecedented, rapid decline in multiple species following WNS may provide an opportunity to observe a dramatic restructuring of the bat community. Location We conducted our study at Fort Drum Army Installation in Jefferson and Lewis counties, New York, USA, where WNS first impacted extant bat species in winter 2007-2008. Methods Acoustical monitoring during 2003-2011 allowed us to test the hypothesis that spatial and temporal niche partitioning by bats was relaxed post-WNS. Results We detected nine bat species pre-and post-WNS. Activity for most bat species declined post-WNS. Dramatic post-WNS declines in activity of little brown bat (Myotis lucifugus, MYLU), formerly the most abundant bat species in the region, were associated with complex, often species-specific responses by other species that generally favoured increased spatial and temporal overlap with MYLU. Main conclusions In addition to the obvious direct effects of disease on bat populations and activity levels, our results provide evidence that disease can have cascading indirect effects on community structure. Recent occurrence of WNS in North America, combined with multiple existing stressors, is resulting in dramatic shifts in temporal and spatial niche partitioning within bat communities. These changes might influence long-term population viability of some bat species as well as broader scale ecosystem structure and function. C1 [Jachowski, David S.; Coleman, Laci S.] Virginia Polytech Inst & State Univ, Dept Fisheries & Wildlife Conservat, Blacksburg, VA 24061 USA. [Dobony, Chris A.] IMNE DRM PWE, Ft Drum Mil Installat, Nat Resources Branch, Ft Drum, NY 13602 USA. [Ford, William M.] Virginia Polytech Inst & State Univ, US Geol Survey, Virginia Cooperat Fish & Wildlife Res Unit, Blacksburg, VA 24061 USA. [Britzke, Eric R.] US Army Res & Dev Ctr, Environm Lab, Vicksburg, MS 39180 USA. [Rodrigue, Jane L.] US Forest Serv, No Res Stn, Princeton, WV 24740 USA. RP Jachowski, DS (reprint author), Virginia Polytech Inst & State Univ, Dept Fisheries & Wildlife Conservat, Blacksburg, VA 24061 USA. EM djachowski@gmail.com FU Department of the Army, Fort Drum; U.S. Army Engineer and Research Development Center; U.S. Forest Service, Northern Research Station; U.S. Geological Survey, Cooperative Fish and Wildlife Research Program FX We thank R. Rainbolt for assistance throughout this project. This work was funded by financial and in-kind contributions of the Department of the Army, Fort Drum, the U.S. Army Engineer and Research Development Center, the U.S. Forest Service, Northern Research Station, and the U.S. Geological Survey, Cooperative Fish and Wildlife Research Program. NR 96 TC 6 Z9 6 U1 11 U2 124 PU WILEY PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1366-9516 EI 1472-4642 J9 DIVERS DISTRIB JI Divers. Distrib. PD SEP PY 2014 VL 20 IS 9 BP 1002 EP 1015 DI 10.1111/ddi.12192 PG 14 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AN2OX UT WOS:000340426300002 ER PT J AU Grove, JM Locke, DH O'Neil-Dunne, JPM AF Grove, J. Morgan Locke, Dexter H. O'Neil-Dunne, Jarlath P. M. TI An Ecology of Prestige in New York City: Examining the Relationships Among Population Density, Socio-economic Status, Group Identity, and Residential Canopy Cover SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Urban ecology; Urban forestry; Private land; Parcel; Geodemographics; Urban tree canopy ID SPATIAL-DISTRIBUTION; URBAN ECOSYSTEMS; LAWN-CARE; VEGETATION; LANDSCAPE; BALTIMORE; GARDENS; LAND; HOMOGENIZATION; NEIGHBORHOODS AB Several social theories have been proposed to explain the uneven distribution of vegetation in urban residential areas: population density, social stratification, luxury effect, and ecology of prestige. We evaluate these theories using a combination of demographic and socio-economic predictors of vegetative cover on all residential lands in New York City. We use diverse data sources including the City's property database, time-series demographic and socio-economic data from the US Census, and land cover data from the University of Vermont's Spatial Analysis Lab (SAL). These data are analyzed using a multi-model inferential, spatial econometrics approach. We also examine the distribution of vegetation within distinct market categories using Claritas' Potential Rating Index for Zipcode Markets (PRIZM (TM)) database. These categories can be disaggregated, corresponding to the four social theories. We compare the econometric and categorical results for validation. Models associated with ecology of prestige theory are more effective for predicting the distribution of vegetation. This suggests that private, residential patterns of vegetation, reflecting the consumption of environmentally relevant goods and services, are associated with different lifestyles and lifestages. Further, our spatial and temporal analyses suggest that there are significant spatial and temporal dependencies that have theoretical and methodological implications for understanding urban ecological systems. These findings may have policy implications. Decision makers may need to consider how to most effectively reach different social groups in terms of messages and messengers in order to advance land management practices and achieve urban sustainability. C1 [Grove, J. Morgan] USDA Forest Serv, Baltimore Field Stn, UMBC, Northern Res Stn, Baltimore, MD 21228 USA. [Locke, Dexter H.] Clark Univ, Grad Sch Geog, Worcester, MA 01610 USA. [O'Neil-Dunne, Jarlath P. M.] Univ Vermont, Spatial Anal Lab, Burlington, VT 05405 USA. RP Grove, JM (reprint author), USDA Forest Serv, Baltimore Field Stn, UMBC, Northern Res Stn, 5522 Res Pk Dr, Baltimore, MD 21228 USA. EM mgrove@fs.fed.us; dexter.locke@gmail.com FU Carpenter-Sperry Award; National Science Foundation [DEB-0423476] FX Most of this work was conducted at the Yale School of Forestry and Environmental Studies, with support from the Carpenter-Sperry Award. This research was also supported by the National Science Foundation grant for the Baltimore Ecosystem Study Long Term Ecological Research site (DEB-0423476). The manuscript was made better with helpful comments provided by Sophie Plitt (NYC Department of Parks & Recreation), Colin Polsky (Clark University), Nancy Falxa-Raymond, Lynne Westphal, (USDA Forest Service, Northern Research Station), Darrel Jenerette (University of California Riverside) and Shawn Landry (University of South Florida). NR 69 TC 16 Z9 17 U1 5 U2 63 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD SEP PY 2014 VL 54 IS 3 BP 402 EP 419 DI 10.1007/s00267-014-0310-2 PG 18 WC Environmental Sciences SC Environmental Sciences & Ecology GA AN4DP UT WOS:000340538100003 PM 25034751 ER PT J AU Toman, E Shindler, B McCaffrey, S Bennett, J AF Toman, Eric Shindler, Bruce McCaffrey, Sarah Bennett, James TI Public Acceptance of Wildland Fire and Fuel Management: Panel Responses in Seven Locations SO ENVIRONMENTAL MANAGEMENT LA English DT Article DE Fuels reduction; Public acceptance; Wildland fire management ID URBAN INTERFACE; PRESCRIBED FIRE; SOCIAL ACCEPTABILITY; REDUCTION STRATEGIES; UNITED-STATES; ATTITUDES; POLICIES; SUPPORT; COMMUNICATION; PERSPECTIVES AB Wildland fire affects both public and private resources throughout the United States. A century of fire suppression has contributed to changing ecological conditions and accumulated fuel loads. Managers have used a variety of approaches to address these conditions and reduce the likelihood of wildland fires that may result in adverse ecological impacts and threaten communities. Public acceptance is a critical component of developing and implementing successful management programs. This study examines the factors that influence citizen support for agency fuel reduction treatments over time-particularly prescribed fire and mechanical vegetation removal. This paper presents findings from a longitudinal study examining resident beliefs and attitudes regarding fire management and fuels treatments in seven states: Arizona, Colorado, Oregon, Utah, Michigan, Minnesota, and Wisconsin. The study was implemented in two phases over a 6-year period using mail surveys to residents of communities adjacent to federal lands in each location. Questions replicated measures from the original project as well as some new items to allow a more in-depth analysis of key concepts. The study design enables comparisons over time as well as between locations. We also assess the factors that influence acceptance of both prescribed fire and mechanical vegetation removal. Findings demonstrate a relative stability of attitudes toward fuels management approaches over time and suggest that this acceptance is strongly influenced by confidence in resource managers and beliefs that the treatments would result in positive outcomes. C1 [Toman, Eric] Ohio State Univ, Sch Environm & Nat Resources, Columbus, OH 43210 USA. [Shindler, Bruce] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [McCaffrey, Sarah] USDA Forest Serv, No Res Stn, Evanston, IL USA. [Bennett, James] Ohio Off Budget & Management, Columbus, OH USA. RP Toman, E (reprint author), Ohio State Univ, Sch Environm & Nat Resources, 210 Kottman Hall,2021 Coffey Rd, Columbus, OH 43210 USA. EM toman.10@osu.edu FU Joint Fire Science Program; USDA Forest Service Northern Research Station FX The authors wish to thank the Joint Fire Science Program and USDA Forest Service Northern Research Station for supporting this research. The authors also thank the reviewers for their thoughtful review and meaningful suggestions. NR 37 TC 4 Z9 4 U1 1 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0364-152X EI 1432-1009 J9 ENVIRON MANAGE JI Environ. Manage. PD SEP PY 2014 VL 54 IS 3 BP 557 EP 570 DI 10.1007/s00267-014-0327-6 PG 14 WC Environmental Sciences SC Environmental Sciences & Ecology GA AN4DP UT WOS:000340538100013 PM 25034754 ER PT J AU Felix, RK Orzell, SL Tillman, EA Engeman, RM Avery, ML AF Felix, Rodney K., Jr. Orzell, Steve L. Tillman, Eric A. Engeman, Richard M. Avery, Michael L. TI Fine-scale, spatial and temporal assessment methods for feral swine disturbances to sensitive plant communities in south-central Florida SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Disturbance; Sus scrofa; Florida endemic plants; Seepage slopes; Flatwoods; Wet prairies ID SUS-SCROFA; DAMAGE; MANAGEMENT; VEGETATION; BIODIVERSITY; OSCILLATION; LANDSCAPE; DYNAMICS; HABITAT; FOREST AB Feral swine are estimated to annually cost hundreds of millions of dollars in economic loss to property and agriculture in the USA, while their ecological consequences remain largely unmeasured. Using submeter-accurate Global Positioning System technology over a multiyear project, we are quantifying in a novel way the spatial and temporal attributes of swine rooting damage within 587 ha of ecologically sensitive wetland plant communities at Avon Park Air Force Range in south-central Florida. We delineated damage polygons from 0.0023 to 4,335 m(2) and were able to document recurrent damage through time at most sites during each assessment. For each polygon, we also estimated the age of damage and assigned to it a severity index, qualities of the rooting in which we detected changes in proportions over time. Spatially explicit damage assessments at fine scales conducted over several years can assist land managers in determining effects of rooting on rare plant populations, and will allow investigators to hypothesize what factors are driving patterns of this disturbance across ecologically sensitive plant communities. C1 [Felix, Rodney K., Jr.; Tillman, Eric A.; Avery, Michael L.] USDA, Natl Wildlife Res Ctr, Florida Field Stn, Gainesville, FL 32641 USA. [Orzell, Steve L.] Avon Pk Air Force Range, Avon Pk, FL 33825 USA. [Engeman, Richard M.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. RP Felix, RK (reprint author), Eglin Air Force Base Nat Resources Wildlife, 107 SR 85 North, Niceville, FL 32578 USA. EM rodney.felix.1@us.af.mil FU APAFR; National Wildlife Research Center [08-7483-0707]; Avon Park Air Force Range [08-7483-0707]; USAF FX We appreciate the support of Paul Ebersbach, Chief, Environmental Flight, APAFR, and the USAF for funding this project; and Gary Killian, coordinator of this project for the USDA/National Wildlife Research Center. For field assistance, we are grateful to J. S. Humphrey, W. E. Bruce, K. L. Keacher, and E. Fetzer, and for comments on an earlier version of this manuscript we thank M. Slocum. This research was supported through interagency agreement 08-7483-0707(IA) between the National Wildlife Research Center and Avon Park Air Force Range. NR 34 TC 4 Z9 4 U1 4 U2 24 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD SEP PY 2014 VL 21 IS 17 BP 10399 EP 10406 DI 10.1007/s11356-014-2956-y PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA AN5HC UT WOS:000340620400036 PM 24793069 ER PT J AU Le, TTY Wang, P Vijver, MG Kinraide, TB Hendriks, AJ Peijnenburg, WJGM AF Le, T. T. Yen Wang, Peng Vijver, Martina G. Kinraide, Thomas B. Hendriks, A. Jan Peijnenburg, Willie J. G. M. TI DELINEATING ION-ION INTERACTIONS BY ELECTROSTATIC MODELING FOR PREDICTING RHIZOTOXICITY OF METAL MIXTURES TO LETTUCE LACTUCA SATIVA SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Metal mixture; Model; Plant; Toxicity; Membrane surface; Interactions ID PLASMA-MEMBRANE SURFACE; BIOTIC LIGAND MODEL; COPPER TOXICITY; DAPHNIA-MAGNA; ZINC; CADMIUM; PH; ALLEVIATION; CALCIUM; BIOAVAILABILITY AB Effects of ion-ion interactions on metal toxicity to lettuce Lactuca sativa were studied based on the electrical potential at the plasma membrane surface (psi(0)). Surface interactions at the proximate outside of the membrane influenced ion activities at the plasma membrane surface ({Mn+}(0)). At a given free Cu2+ activity in the bulk medium ({Cu2+}(b)), additions of Na+, K+, Ca2+, and Mg2+ resulted in substantial decreases in {Cu2+}(0). Additions of Zn2+ led to declines in {Cu2+}(0), but Cu2+ and Ag+ at the exposure levels tested had negligible effects on the plasma membrane surface activity of each other. Metal toxicity was expressed by the {Mn+}(0)-based strength coefficient, indicating a decrease of toxicity in the order: Ag+> Cu2+> Zn-2+. Adsorbed Na+, K+, Ca2+, and Mg2+ had significant and dose-dependent effects on Cu2+ toxicity in terms of osmolarity. Internal interactions between Cu2+ and Zn2+ and between Cu2+ and Ag+ were modeled by expanding the strength coefficients in concentration addition and response multiplication models. These extended models consistently indicated that Zn2+ significantly alleviated Cu2+ toxicity. According to the extended concentration addition model, Ag+ significantly enhanced Cu2+ toxicity whereas Cu2+ reduced Ag+ toxicity. By contrast, the response multiplication model predicted insignificant effects of adsorbed Cu2+ and Ag+ on the toxicity of each other. These interactions were interpreted using psi(0), demonstrating its influence on metal toxicity. (c) 2014 SETAC C1 [Le, T. T. Yen; Hendriks, A. Jan] Radboud Univ Nijmegen, Dept Environm Sci, Inst Water & Wetland Res, NL-6525 ED Nijmegen, Netherlands. [Le, T. T. Yen; Peijnenburg, Willie J. G. M.] Natl Inst Publ Hlth & Environm, Lab Ecol Risk Assessment, NL-3720 BA Bilthoven, Netherlands. [Wang, Peng] Univ Queensland, Sch Agr & Food Sci, St Lucia, Qld, Australia. [Vijver, Martina G.; Peijnenburg, Willie J. G. M.] Leiden Univ, Dept Conservat Biol, Inst Environm Sci CML, Leiden, Netherlands. [Kinraide, Thomas B.] ARS, USDA, Beaver, WV USA. RP Le, TTY (reprint author), Radboud Univ Nijmegen, Dept Environm Sci, Inst Water & Wetland Res, NL-6525 ED Nijmegen, Netherlands. EM yenle@science.ru.nl RI Wang, Peng/E-2008-2012; Hendriks, Aalbert Jan/C-6767-2013; OI Wang, Peng/0000-0001-8622-8767; peijnenburg, willie/0000-0003-2958-9149 NR 45 TC 2 Z9 2 U1 0 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD SEP PY 2014 VL 33 IS 9 BP 1988 EP 1995 DI 10.1002/etc.2643 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA AN4DI UT WOS:000340537400011 PM 24863101 ER PT J AU Stern, JA White, SN Lehmkuhl, LB Reina-Doreste, Y Ferguson, JL Nascone-Yoder, NM Meurs, KM AF Stern, Joshua A. White, Stephen N. Lehmkuhl, Linda B. Reina-Doreste, Yamir Ferguson, Jordan L. Nascone-Yoder, Nanette M. Meurs, Kathryn M. TI A single codon insertion in PICALM is associated with development of familial subvalvular aortic stenosis in Newfoundland dogs SO HUMAN GENETICS LA English DT Article ID SUBAORTIC STENOSIS; INHERITANCE AB Familial subvalvular aortic stenosis (SAS) is one of the most common congenital heart defects in dogs and is an inherited defect of Newfoundlands, golden retrievers and human children. Although SAS is known to be inherited, specific genes involved in Newfoundlands with SAS have not been defined. We hypothesized that SAS in Newfoundlands is inherited in an autosomal dominant pattern and caused by a single genetic variant. We studied 93 prospectively recruited Newfoundland dogs, and 180 control dogs of 30 breeds. By providing cardiac screening evaluations for Newfoundlands we conducted a pedigree evaluation, genome-wide association study and RNA sequence analysis to identify a proposed pattern of inheritance and genetic loci associated with the development of SAS. We identified a three-nucleotide exonic insertion in phosphatidylinositol-binding clathrin assembly protein (PICALM) that is associated with the development of SAS in Newfoundlands. Pedigree evaluation best supported an autosomal dominant pattern of inheritance and provided evidence that equivocally affected individuals may pass on SAS in their progeny. Immunohistochemistry demonstrated the presence of PICALM in the canine myocardium and area of the subvalvular ridge. Additionally, small molecule inhibition of clathrin-mediated endocytosis resulted in developmental abnormalities within the outflow tract (OFT) of Xenopus laevis embryos. The ability to test for presence of this PICALM insertion may impact dog-breeding decisions and facilitate reduction of SAS disease prevalence in Newfoundland dogs. Understanding the role of PICALM in OFT development may aid in future molecular and genetic investigations into other congenital heart defects of various species. C1 [Stern, Joshua A.; Reina-Doreste, Yamir; Meurs, Kathryn M.] N Carolina State Univ, Dept Clin Sci, Coll Vet Med, Raleigh, NC 27607 USA. [Stern, Joshua A.; Meurs, Kathryn M.] Washington State Univ, Dept Veterinary Clin Sci, Coll Vet Med, Pullman, WA 99164 USA. [Stern, Joshua A.] Univ Calif Davis, Sch Vet Med, Dept Med & Epidemiol, Davis, CA 95616 USA. [White, Stephen N.] ARS, Anim Dis Res Unit, USDA, Pullman, WA USA. [White, Stephen N.] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Lehmkuhl, Linda B.] MedVet Med Ctr Pets, Worthington, OH 43085 USA. [Lehmkuhl, Linda B.] MedVet Canc Ctr Pets, Worthington, OH 43085 USA. [Ferguson, Jordan L.; Nascone-Yoder, Nanette M.] N Carolina State Univ, Dept Mol Biomed Sci, Coll Vet Med, Raleigh, NC 27607 USA. RP Stern, JA (reprint author), Univ Calif Davis, Sch Vet Med, Dept Med & Epidemiol, 2108 Tupper Hall,One Shields Ave, Davis, CA 95616 USA. EM jstern@ucdavis.edu OI White, Stephen N./0000-0001-9255-6054 FU Morris Animal Foundation-Pfizer Animal Health fellowship; University of California Davis, School of Veterinary Medicine, Center for Companion Animal Health; Morris Animal Foundation; Newfoundland Club of America FX Dr. Joshua Stern's research training program was supported by a Morris Animal Foundation-Pfizer Animal Health fellowship training grant. Publication costs were supported by the University of California Davis, School of Veterinary Medicine, Center for Companion Animal Health. The authors would like to acknowledge Morris Animal Foundation for their generous grant support of this project and the Newfoundland Club of America for their financial support and aid in recruiting study dogs. NR 27 TC 2 Z9 2 U1 0 U2 18 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0340-6717 EI 1432-1203 J9 HUM GENET JI Hum. Genet. PD SEP PY 2014 VL 133 IS 9 BP 1139 EP 1148 DI 10.1007/s00439-014-1454-0 PG 10 WC Genetics & Heredity SC Genetics & Heredity GA AN3WT UT WOS:000340520100007 PM 24898977 ER PT J AU Flores-Silva, PC Berrios, JD Pan, J Osorio-Diaz, P Bello-Perez, LA AF Flores-Silva, Pamela C. Berrios, Jose De J. Pan, James Osorio-Diaz, Perla Bello-Perez, Luis A. TI Gluten-free spaghetti made with chickpea, unripe plantain and maize flours: functional and chemical properties and starch digestibility SO INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Cooking quality; gluten-free; pasta; plantain; starch digestibility ID RESISTANT STARCH; BANANA STARCH; GLYCEMIC INDEX; SMALL-INTESTINE; CELIAC-DISEASE; BLOOD-GLUCOSE; PASTA; QUALITY; LEGUMES; FIBER AB The development of gluten-free spaghetti with a low amount of glycaemic carbohydrate was investigated. The goal of this study was to determine the chemical composition, cooking quality and starch digestibility of gluten-free spaghetti elaborated with mixtures of chickpea, unripe plantain and maize flours. The gluten-free spaghetti presented a higher protein, fat and ash content than the control semolina spaghetti. The solid loss among all the gluten-free spaghetti was in the range of 10.04-10.91% and not significantly different from each other. These values were almost at the limit of acceptability to be considered as good cooking quality. Total starch in the gluten-free spaghetti was lower than the control spaghetti. The lower available starch (AS) and higher resistant starch contents in the gluten-free spaghetti were associated with their lower rate of hydrolysis and predicted glycaemic index. There is a potential for developing gluten-free spaghetti with reduced amount of glycaemic carbohydrates from unconventional food ingredients such as chickpea, unripe plantain and maize flours. C1 [Flores-Silva, Pamela C.; Osorio-Diaz, Perla; Bello-Perez, Luis A.] Inst Politecn Nacl, CEPROBI, Yautepec 62731, Morelos, Mexico. [Berrios, Jose De J.; Pan, James] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Bello-Perez, LA (reprint author), Inst Politecn Nacl, CEPROBI, Apartado Postal 24, Yautepec 62731, Morelos, Mexico. EM labellop@ipn.mx FU SIP-IPN; EDI-IPN CONACYT-Mexico; COFAA-IPN; CONACYT-Mexico FX We appreciate the economic support from SIP-IPN, EDI-IPN CONACYT-Mexico and COFAA-IPN. One of the authors (PCFS) also acknowledges the scholarship from CONACYT-Mexico. Support by Sam Huang from California Wheat Commission, to use his facilities is also thanked. NR 47 TC 18 Z9 18 U1 6 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0950-5423 EI 1365-2621 J9 INT J FOOD SCI TECH JI Int. J. Food Sci. Technol. PD SEP PY 2014 VL 49 IS 9 BP 1985 EP 1991 DI 10.1111/ijfs.12529 PG 7 WC Food Science & Technology SC Food Science & Technology GA AN4BV UT WOS:000340533500003 ER PT J AU Bennett, VJ Betts, MG Smith, WP AF Bennett, V. J. Betts, M. G. Smith, W. P. TI Influence of thermal conditions on habitat use by a rare spring-emerging butterfly Euphydryas editha taylori SO JOURNAL OF APPLIED ENTOMOLOGY LA English DT Article DE adult flight period; habitat quality indicator; in situ habitat use; larval distribution; Taylor's checkerspot butterfly; thermal thresholds ID CHECKERSPOT BUTTERFLIES; METAPOPULATION DYNAMICS; POPULATION-DYNAMICS; HERBIVOROUS INSECTS; BASKING POSTURE; PARARGE-AEGERIA; CLIMATE-CHANGE; CONSERVATION; LEPIDOPTERA; NYMPHALIDAE AB We may expect butterflies as ectotherms to have particularly active life-history stages that occur in the warmest and lightest times of the year; however, there are temperate species that are active when climatic conditions seem unfavourable and photoperiod short, such as the Taylor's checkerspot (Euphydryas editha taylori). For such species, studies suggest that even subtle changes to microclimate can potentially impact populations. Thus, understanding how in situ variations in microclimate influence the Taylor's checkerspot butterfly could provide much needed insights into more effective management. We conducted a series of surveys that explored (i) adult habitat use, (ii) final instar larval distribution and (iii) adult movement up to and across site boundaries at two sites in Oregon, USA, in 2010 and 2011. We found that in situ habitat use by the Taylor's checkerspot butterfly was strongly influenced by microclimate. Both adult activities and final instar larvae distribution were clustered within the warmest areas of the sites. Moreover, adults did not use up to 59% and larva up to 90% of their sites, despite vegetation structure and composition being uniform. More specifically, butterfly habitat use increased with increasing ground temperatures, and we found that areas with the highest ground temperatures were more exposed to direct sunlight. Similarly, we found that butterflies tended to only move through sunlit site boundaries. We conclude that the Taylor's checkerspot is sensitive to changes in its thermal environment at fine spatial scales. Our results highlight the importance of microclimate as an indicator of habitat quality, and establishing the thermal criteria in which species of concern exists may provide valuable insights into the implications of climate change. C1 [Bennett, V. J.] Texas Christian Univ, Sch Geol Energy & Environm, Ft Worth, TX 76129 USA. [Bennett, V. J.; Betts, M. G.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Smith, W. P.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Olympia, WA USA. RP Bennett, VJ (reprint author), Texas Christian Univ, Sch Geol Energy & Environm, Ft Worth, TX 76129 USA. EM v.bennett@tcu.edu FU Oregon State University's General Research Fund FX Thanks to Oregon State University's General Research Fund for funding this project. Special thanks to Al Kitzman, superintendent of Benton County Natural Areas and Parks in Oregon and Karen Holtrop and Susan Piper of the Olympic National Forest, Forest Service in Washington for providing the opportunity, supporting and encouraging our research on the Taylor's to be carried out at their sites. Many thanks to Shalynn Pack, Maria Rocco, Diana Raper, Katelynn Karch, Aaron McAlexander, James Lafferty, Susan Shirley and Yuon Han for providing much needed assistance in data collection. Additional thanks to Diana Raper, Karen Holtrop and Ann Potter of the Washington Department of Fish and Wildlife for providing feedback on manuscript drafts. NR 37 TC 2 Z9 2 U1 10 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2048 EI 1439-0418 J9 J APPL ENTOMOL JI J. Appl. Entomol. PD SEP PY 2014 VL 138 IS 8 BP 623 EP 634 DI 10.1111/jen.12137 PG 12 WC Entomology SC Entomology GA AN4PE UT WOS:000340569300007 ER PT J AU Motamed, MJ Florax, RJGM Masters, WA AF Motamed, Mesbah J. Florax, Raymond J. G. M. Masters, William A. TI Agriculture, transportation and the timing of urbanization: Global analysis at the grid cell level SO JOURNAL OF ECONOMIC GROWTH LA English DT Article DE Economic growth; Economic geography; Urbanization; Agriculture; Transportation ID ECONOMIC-GROWTH; SPATIALLY EXPLICIT; HYDE 3.1; POPULATION; LAND; REVOLUTION; GEOGRAPHY; PRODUCTIVITY; CLIMATE; MACROECONOMICS AB This paper addresses the timing of a location's historical transition from rural to urban activity. We test whether urbanization occurs sooner in places with higher agricultural potential and comparatively lower transport costs, using worldwide data that divide the earth's surface at half-degree intervals into 62,290 cells. From an independent estimate of each cell's rural and urban population history over the last 2,000 years, we identify the date at which each cell achieves various thresholds of urbanization. Controlling for unobserved heterogeneity across countries through fixed effects and using a variety of spatial econometric techniques, we find a robust association between earlier urbanization and agro-climatic suitability for cultivation, having seasonal frosts, better access to the ocean or navigable rivers, and lower elevation. These geographic correlations become smaller in magnitude as urbanization proceeds, and there is some variation in the effects across continents. Aggregating cells into countries, we show that an earlier urbanization date is associated with higher per capita income today. C1 [Motamed, Mesbah J.] USDA, Econ Res Serv, Washington, DC USA. [Florax, Raymond J. G. M.] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. [Florax, Raymond J. G. M.] Vrije Univ Amsterdam, Dept Spatial Econ, Amsterdam, Netherlands. [Florax, Raymond J. G. M.] Tinbergen Inst, Amsterdam, Netherlands. [Masters, William A.] Tufts Univ, Friedman Sch Nutr, Boston, MA 02111 USA. [Masters, William A.] Tufts Univ, Dept Econ, Boston, MA 02111 USA. RP Masters, WA (reprint author), Tufts Univ, Friedman Sch Nutr, Boston, MA 02111 USA. EM mmotamed@ers.usda.gov; william.masters@tufts.edu RI Masters, William/A-4796-2017 OI Masters, William/0000-0002-4546-7291 NR 73 TC 5 Z9 5 U1 6 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1381-4338 EI 1573-7020 J9 J ECON GROWTH JI J. Econ. Growth PD SEP PY 2014 VL 19 IS 3 BP 339 EP 368 DI 10.1007/s10887-014-9104-x PG 30 WC Economics SC Business & Economics GA AN3YG UT WOS:000340524200003 ER PT J AU Masler, EP AF Masler, E. P. TI Effects of catechin polyphenols and preparations from the plant-parasitic nematode Heterodera glycines on protease activity and behaviour in three nematode species SO JOURNAL OF HELMINTHOLOGY LA English DT Article ID SOYBEAN CYST-NEMATODE; IN-VITRO; TEA POLYPHENOLS; ROSTOCHIENSIS; COMPONENTS; INHIBITOR; ESTER; EGGS AB Protease activities in preparations from the plant-parasitic nematodes Heterodera glycines and Meloidogyne incognita and the free-living nematode Panagrellus redivivus were inhibited by exposure to a series of eight catechin polyphenol analogues, (+)-catechin, (-)-epicatechin (EC), (-)-gallocatechin (GC), (-)-epigallocatechin (EGC), (-)-catechin gallate (CG), (-)-gallocatechin gallate (GCG), (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG) (1 mM each), and by a preparation from H. glycines cysts. General protease activity detected with the FRET-peptide substrate QXL520-KSAYMRF-K (5-FAM) a and proteasome chymotrypsin-like (CTL) activity detected with succinyl-LLVY-AMC were each inhibited significantly more (P < 0.05) by the gallated form of the polyphenol than by the corresponding non-gallated form. Species differences in response to inhibition across all analogues were revealed with the CTL substrate, but CG was a consistently potent inhibitor across all three species and with each substrate. A heat-stable component (CE) from H. glycines cysts inhibited M. incognita CTL activity by 92.07 +/- 0.68%, significantly less (P < 0.05) in H. glycines (52.86 +/- 2.77%), and by only 17.24 +/- 0.55% (P < 0.05) in P. redivivus preparations. CTL activity was, however, inhibited more than 60% in all preparations by the proteasome-specific inhibitor MG-132. Hatching of M. incognita infective juveniles exposed to 1 mM CG, ECG, GCG or EGCG was reduced by 83.88 +/- 4.26%, 69.98 +/- 9.14%, 94.93 +/- 1.71% and 87.93 +/- 2.89%, respectively, while hatching of H. glycines was reduced less than 25% by each analogue. CE had no effect on nematode hatch, but did cause a 60% reduction in mobility of H. glycines infective juveniles exposed overnight to CE in vitro, which was more (P < 0.05) than the reduction of M. incognita infective juvenile mobility (20%). C1 ARS, Nematol Lab US, USDA, Beltsville, MD 20705 USA. RP Masler, EP (reprint author), ARS, Nematol Lab US, USDA, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM edward.masler@ars.usda.gov NR 23 TC 2 Z9 2 U1 0 U2 12 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0022-149X EI 1475-2697 J9 J HELMINTHOL JI J. Helminthol. PD SEP PY 2014 VL 88 IS 3 BP 349 EP 356 DI 10.1017/S0022149X13000254 PG 8 WC Parasitology; Zoology SC Parasitology; Zoology GA AN4MS UT WOS:000340562700012 PM 23635519 ER PT J AU Kuenen, LPS Gilbert, C Siegel, J AF Kuenen, Lodewyk P. S. Gilbert, Cole Siegel, Joel TI Flying Slower: Floor Pattern Object Size Affects Orthokinetic Responses During Moth Flight to Sex Pheromone SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Flight speed; visual patterns; moths; sex pheromone; vision ID ORIENTAL FRUIT MOTH; LYMANTRIA-DISPAR L; UPWIND FLIGHT; GRAPHOLITHA-MOLESTA; GYPSY-MOTH; OPTOMOTOR ANEMOTAXIS; DIFFERENT HEIGHTS; SOURCE LOCATION; WIND-TUNNEL; LEPIDOPTERA AB Previous studies with Oriental Fruit Moth (OFM, Grapholita molesta) and Heliothis virescens males flying upwind along a pheromone plume showed that they increased their upwind flight speed as they flew higher above striped floor patterns and, for OFM, to a similar degree over dotted floor patterns. This response pattern has been demonstrated in another moth species, Epiphyas postvittana and in a beetle, Prostephanus truncatus. In all cases the role played by the change in angular size of the wind tunnel's ventral floor pattern was not assessed. In the present study we specifically addressed this question with a systematic examination of moths' flight control over different sizes of transverse stripes and dot patterns ranging down by halves from 5 to 0.625 cm and a blank white floor as a control, and showed that OFM males fly faster upwind and along their flight paths over floor patterns of decreasing size. Increased speeds over striped patterns were evident as stripe width decreased below 2.5 cm, whereas moths did not increase their flight speed over dot patterns until dot size had decreased to less than 1.25 cm. Another flight component that the moths can actively control, their course angles, was unchanged above both patterns, except for moths flying over 5 cm stripes. Turning frequency and interturn distances were mostly unchanged or offset each other, negating any effects on upwind progress. As in an earlier study examining flight speeds at three heights above floor patterns of three densities, the moths' changes in speed appear to be exclusively affected by changes in their orthokinetic response to the size of the floor pattern objects. C1 [Gilbert, Cole] Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA. [Kuenen, Lodewyk P. S.; Siegel, Joel] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Kuenen, LPS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM bas.kuenen@ars.usda.gov FU California Pistachio Research Board FX We thank Sarah Hofer for assistance in rearing insects, making the floor patterns and digitizing the moth tracks. This work was funded in part by funds from the California Pistachio Research Board. NR 43 TC 2 Z9 2 U1 3 U2 16 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD SEP PY 2014 VL 27 IS 5 BP 581 EP 592 DI 10.1007/s10905-014-9450-8 PG 12 WC Entomology SC Entomology GA AN3OT UT WOS:000340498500003 ER PT J AU Lee, DH Nielsen, AL Leskey, TC AF Lee, Doo-Hyung Nielsen, Anne L. Leskey, Tracy C. TI Dispersal Capacity and Behavior of Nymphal Stages of Halyomorpha halys (Hemiptera: Pentatomidae) Evaluated Under Laboratory and Field Conditions SO JOURNAL OF INSECT BEHAVIOR LA English DT Article DE Brown marmorated stink bug; invasive species; movement; mark-release-recapture ID BUGS HETEROPTERA-PENTATOMIDAE; PLUM CURCULIO COLEOPTERA; BAITED TRAP TREES; INVASIVE PEST; IMPACT; ODOR; MANAGEMENT; PHEROMONE; SOYBEANS; MOBILITY AB The invasive brown marmorated stink bug, Halyomorpha halys (StAyenl) (Hemiptera: Pentatomidae), is a highly polyphagous and mobile pest causing crop damage aggregated at the perimeters of crop fields. Understanding the dispersal biology of H. halys is critical for the development of reliable monitoring and management strategies. In this study, dispersal ecology of H. halys nymphs was studied under laboratory and field conditions. In the laboratory, horizontal and vertical walking capacity was quantified for mobile nymphal stages (i.e., 2nd through 5th instars) and compared with adults. There was a significant difference in the horizontal distance moved by H. halys among the life stages tested. Third instars exhibited significantly greater walking distances compared with adults; horizontal walking distances by other nymphal stages were not significantly different from adults. A similar pattern was observed from vertical climbing tests of H. halys. Third and 4th instars climbed significantly greater distances compared with 2nd instars and adults, while distances climbed by 5th instars were intermediate. In the field, the walking distance of 3rd and 5th instar nymphs on mowed grass was quantified based on direct observation of individuals for 30 min. Under these conditions, 5th instars moved nearly two-fold greater distances compared with 3rd instars, but surface temperature affected both nymphal stages similarly. Shorter bouts of movement were common at surface temperatures below 25 A degrees C, whereas individuals showed longer walking distances above 25 A degrees C. In mark-release-recapture studies, 4th and 5th instars were released and recaptured in traps baited with attractive pheromonal-based stimuli to estimate dispersal rates under field conditions. When insects were released 5 m from traps, both instars were first recaptured within 2 h after release, with the recapture rates of 54 and 69 % for 4th and 5th instars over 24 h, respectively. When insects were released 20 m from traps, 4th and 5th instars were first recaptured in less than 5 h, with the recapture rates of 27 and 51 %, respectively. The results of this study indicate that H. halys nymphs have strong dispersal capacity with which populations can easily move among host plants and other attractive stimuli at farmscape levels. C1 [Lee, Doo-Hyung] Gachon Univ, Dept Life Sci, Songnam, Gyeonggi Do, South Korea. [Nielsen, Anne L.] Rutgers Agr Res & Extens Ctr, Bridgeton, NJ 08302 USA. [Leskey, Tracy C.] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. RP Lee, DH (reprint author), Gachon Univ, Dept Life Sci, 1342 Seongnam Daero, Songnam, Gyeonggi Do, South Korea. EM dl343@gachon.ac.kr FU USDA-NIFA OREI [2012-51300-20097] FX We thank Cameron Scorza, John Cullum, and Samuel Nathan Brandt for excellent technical support. We thank Clarissa Mathews and Haroun Hallack for allowing us to conduct mark-release-recapture studies at Redbud Farm in Inwood, WV. This work was supported in part by USDA-NIFA OREI # 2012-51300-20097 award. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 39 TC 12 Z9 13 U1 9 U2 49 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0892-7553 EI 1572-8889 J9 J INSECT BEHAV JI J. Insect Behav. PD SEP PY 2014 VL 27 IS 5 BP 639 EP 651 DI 10.1007/s10905-014-9456-2 PG 13 WC Entomology SC Entomology GA AN3OT UT WOS:000340498500007 ER PT J AU Jong, L Peterson, SC Jackson, MA AF Jong, Lei Peterson, Steven C. Jackson, Michael A. TI Utilization of Porous Carbons Derived from Coconut Shell and Wood in Natural Rubber SO JOURNAL OF POLYMERS AND THE ENVIRONMENT LA English DT Article DE Porous carbons; Natural rubber; Mechanical properties; Polymer-filler interactions ID FILLER INTERACTIONS; X-RAY; TEMPERATURE; ADSORPTION; BEHAVIOR AB The porous carbons derived from cellulose are renewable and environmentally friendly. Coconut shell and wood derived porous carbons were characterized with elemental analysis, ash content, X-ray diffraction, infrared absorbance, particle size, surface area, and pore volume. The results were compared with carbon black. Uniaxial deformation of natural rubber (NR) composites indicate the composites reinforced with the porous carbon from coconut shell have higher tensile moduli at the same elongation ratio than the composites reinforced with wood carbon. 40 % coconut shell composite showed a fivefold increase in tensile modulus compared to NR. Polymer-filler interactions were studied with frequency dependent shear modulus, swelling experiments and dynamic strain sweep experiments. Both linear and non-linear viscoelastic properties indicate the polymer-filler interactions are similar between coconut shell carbon and wood carbon reinforced composites. The swelling experiments, however, showed that the polymer-filler interaction is greater in the composites reinforced with coconut shell instead of wood carbon. C1 [Jong, Lei; Peterson, Steven C.] USDA, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Jackson, Michael A.] USDA, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Jong, L (reprint author), USDA, Plant Polymer Res Unit, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 33 TC 5 Z9 5 U1 3 U2 27 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1566-2543 EI 1572-8900 J9 J POLYM ENVIRON JI J. Polym. Environ. PD SEP PY 2014 VL 22 IS 3 BP 289 EP 297 DI 10.1007/s10924-013-0637-4 PG 9 WC Engineering, Environmental; Polymer Science SC Engineering; Polymer Science GA AN2FQ UT WOS:000340399900002 ER PT J AU Cai, ZJ Wang, BR Xu, MG Zhang, HM Zhang, L Gao, SD AF Cai, Zejiang Wang, Boren Xu, Minggang Zhang, Huimin Zhang, Lu Gao, Suduan TI Nitrification and acidification from urea application in red soil (Ferralic Cambisol) after different long-term fertilization treatments SO JOURNAL OF SOILS AND SEDIMENTS LA English DT Article DE Manure; Maximal nitrification rate (K-max); Nitrification; Red soil; Soil acidification; Urea ID NITROGEN-FERTILIZER; INITIAL PH; CHINA; WHEAT; FIELD AB Long-term manure applications can prevent or reverse soil acidification by chemical nitrogen (N) fertilizer. However, the resistance to re-acidification from further chemical fertilization is unknown. The aim of this study was to examine the effect of urea application on nitrification and acidification processes in an acid red soil (Ferralic Cambisol) after long-term different field fertilization treatments. Soils were collected from six treatments of a 19-year field trial: (1) non-fertilization control, (2) chemical phosphorus and potassium (PK), (3) chemical N only (N), (4) chemical N, P, and K (NPK), (5) pig manure only (M), and (6) NPK plus M (NPKM; 70 % N from M). In a 35-day laboratory incubation experiment, the soils were incubated and examined for changes in pH, NH4 (+), and NO3 (-), and their correlations from urea application at 80 mg N kg(-1)(-80) compared to 0 rate (-0). From urea addition, manure-treated soils exhibited the highest acidification and nitrification rates due to high soil pH (5.75-6.38) and the lowest in the chemical N treated soils due to low soil pH (3.83-3.90) with no N-treated soils (pH 4.98-5.12) fell between. By day 35, soil pH decreased to 5.21 and 5.81 (0.54 and 0.57 unit decrease) in the NPKM-80 and M-80 treatments, respectively, and to 4.69 and 4.53 (0.43 and 0.45 unit decrease) in the control-80 and PK-80 treatments, respectively, with no changes in the N-80 and NPK-80 treatments. The soil pH decrease was highly correlated with nitrification potential, and the estimated net proton released. The maximum nitrification rates (K (max)) of NPKM and M soils (14.7 and 21.6 mg N kg(-1) day(-1), respectively) were significantly higher than other treatments (2.86-3.48 mg N kg(-1) day(-1)). The priming effect on mineralization of organic N was high in manure treated soils. Field data have shown clearly that manure amendment can prevent or reverse the acidification of the red soil. When a chemical fertilizer such as urea is applied to the soil again, however, soil acidification will occur at possibly high rates. Thus, the strategy in soil N management is continuous incorporation of manure to prevent acidification to maintain soil productivity. Further studies under field conditions are needed to provide more accurate assessments on acidification rate from chemical N fertilizer applications. C1 [Cai, Zejiang; Wang, Boren; Xu, Minggang; Zhang, Huimin; Zhang, Lu] Chinese Acad Agr Sci, Minist Agr Key Lab Crop Nutr & Fertilizat, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. [Cai, Zejiang; Wang, Boren; Xu, Minggang; Zhang, Huimin; Zhang, Lu] Qiyang Agroecosyst Natl Field Expt Stn, Qiyang 426182, Hunan, Peoples R China. [Gao, Suduan] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Xu, MG (reprint author), Chinese Acad Agr Sci, Minist Agr Key Lab Crop Nutr & Fertilizat, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. EM mgxu@caas.ac.cn FU National Natural Science Foundation of China [41301309]; National Basic Research Program [2014CB441001]; National Nonprofit Institute Research Grant of CAAS [IARRP-2014-10] FX This study was funded by the National Natural Science Foundation of China (41301309), the National Basic Research Program (2014CB441001) and National Nonprofit Institute Research Grant of CAAS (IARRP-2014-10). Mention of trademark, propriety product, or vendor in this article does not constitute a guarantee or warranty of the product by the CAAS and the USDA-ARS nor does it imply approval to the exclusion of other products or vendors that may be suitable. NR 40 TC 10 Z9 11 U1 9 U2 58 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1439-0108 EI 1614-7480 J9 J SOIL SEDIMENT JI J. Soils Sediments PD SEP PY 2014 VL 14 IS 9 BP 1526 EP 1536 DI 10.1007/s11368-014-0906-4 PG 11 WC Environmental Sciences; Soil Science SC Environmental Sciences & Ecology; Agriculture GA AN4AP UT WOS:000340530300002 ER PT J AU Owen, JP Vander Vliet, A Scoles, GA AF Owen, J. P. Vander Vliet, A. Scoles, G. A. TI Comparative off-host survival of larval Rocky Mountain Wood Ticks (Dermacentor andersoni) collected from ecologically distinct field populations SO MEDICAL AND VETERINARY ENTOMOLOGY LA English DT Article DE Anaplasma; abiotic effects; geographical variation; ixodid tick; stress ID ADULT LIFE STAGE; IXODIDAE; ACARI; DYNAMICS; COLORADO; ECOLOGY; CLIMATE AB Dermacentor andersoni (Ixodida: Ixodidae) Stiles, also known as the Rocky Mountain Wood Tick (RMWT), is found throughout the western United States and transmits pathogens of importance to human and animal health. The distributions and activity patterns of RMWTs are shaped by regional climatic variation. However, it is unknown if responses to climatic variation differ across the tick's geographical range. The objective of this narrow study was to test the hypothesis that the responses of RMWTs to abiotic conditions [e.g. temperature and RH (relative humidity)] vary among populations. We collected RMWTs from ecologically distinct field sites in the states of Montana and Oregon (USA). In the laboratory, we tracked weekly survival of tick larvae under four combinations of RH (75% and 98%) and temperature (26 and 32 degrees C) that reflected the range of conditions observed in the source habitats during spring-summer. For both populations, larval survival time decreased at the higher ambient temperature (50% mortality 1-2weeks earlier). Differences in RH did not affect the survival time of larvae from Oregon. By contrast, the survival time of larvae from Montana decreased at the lower RH (50% mortality 1 week earlier). These data suggest that the tolerance limits for water stress may differ among populations of D. andersoni C1 [Owen, J. P.; Vander Vliet, A.] Washington State Univ, Dept Entomol, Pullman, WA 99164 USA. [Scoles, G. A.] Washington State Univ, USDA ARS, Anim Dis Unit, Pullman, WA 99164 USA. RP Owen, JP (reprint author), Washington State Univ, 166 FSHN,100 Dairy Rd, Pullman, WA 99164 USA. EM jowen@wsu.edu NR 15 TC 1 Z9 1 U1 3 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0269-283X EI 1365-2915 J9 MED VET ENTOMOL JI Med. Vet. Entomol. PD SEP PY 2014 VL 28 IS 3 BP 341 EP 344 DI 10.1111/mve.12049 PG 4 WC Entomology; Veterinary Sciences SC Entomology; Veterinary Sciences GA AN2MT UT WOS:000340420500014 PM 24665893 ER PT J AU Wen, ZF Yang, YZ Zhang, JJ Wang, XP Singer, S Liu, ZC Yang, YJ Yan, GH Liu, ZR AF Wen, Zhifeng Yang, Yazhou Zhang, Jinjin Wang, Xiping Singer, Stacy Liu, Zhongchi Yang, Yingjun Yan, Guohua Liu, Zongrang TI Highly interactive nature of flower-specific enhancers and promoters, and its potential impact on tissue-specific expression and engineering of multiple genes or agronomic traits SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE enhancer/promoter-promoter interaction; tissue-specific expression; ablation; transgenic plants ID TRANSGENE INTEGRATION PATTERNS; MADS-BOX GENE; PLANT TRANSFORMATION; ARABIDOPSIS-THALIANA; RICE PLANTS; 2ND INTRON; VIRUS; ORGANIZATION; TOBACCO; INHERITANCE AB Molecular stacking enables multiple traits to be effectively engineered in crops using a single vector. However, the co-existence of distinct plant promoters in the same transgenic unit might, like their mammalian counterparts, interfere with one another. In this study, we devised a novel approach to investigate enhancer-promoter and promoter-promoter interactions in transgenic plants and demonstrated that three of four flower-specific enhancer/promoters were capable of distantly activating a pollen-and stigma-specific Pps promoter (fused to the cytotoxic DT-A gene) in other tissues, as revealed by novel tissue ablation phenotypes in transgenic plants. The NtAGI1 enhancer exclusively activated stamen-and carpel-specific DT-A expression, thus resulting in tissue ablation in an orientation-independent manner; this activation was completely abolished by the insertion of an enhancer-blocking insulator (EXOB) between the NtAGI1 enhancer and Pps promoter. Similarly, AGL8 and AP1Lb1, but not AP1La, promoters also activated distinct tissue-specific DT-A expression and ablation, with the former causing global growth retardation and the latter ablating apical inflorescences. While the tissue specificity of the enhancer/promoters generally defined their activation specificities, the strength of their activity in particular tissues or developmental stages appeared to determine whether activation actually occurred. Our findings provide the first evidence that plant-derived enhancer/promoters can distantly interact/interfere with one another, which could pose potential problems for the tissue-specific engineering of multiple traits using a single-vector stacking approach. Therefore, our work highlights the importance of adopting enhancer-blocking insulators in transformation vectors to minimize promoter-promoter interactions. The practical and fundamental significance of these findings will be discussed. C1 [Wen, Zhifeng; Yang, Yazhou; Zhang, Jinjin; Wang, Xiping; Yang, Yingjun; Yan, Guohua; Liu, Zongrang] USDA ARS, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA. [Wen, Zhifeng; Yang, Yazhou; Wang, Xiping] Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling, Shaanxi, Peoples R China. [Zhang, Jinjin] Shaanxi Normal Univ, Coll Life Sci, Xian, Shaanxi, Peoples R China. [Singer, Stacy] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. [Liu, Zhongchi] Univ Maryland, Dept Cell Biol & Mol Genet, College Pk, MD 20742 USA. [Yang, Yingjun] Henan Univ Sci & Technol, Coll Forestry, Luoyang, Henan, Peoples R China. [Yan, Guohua] Beijing Acad Agr & Forestry Sci, Inst Forestry & Pomol, Beijing, Peoples R China. RP Liu, ZR (reprint author), USDA ARS, Appalachian Fruit Res Stn, 45 Wiltshire Rd, Kearneysville, WV 25430 USA. EM zongrang.liu@ars.usda.gov FU USDA NIFA Biotechnology Risk Assessment Research Grant [2009-01067] FX We thank Mr. Dennis Bennett for his excellent technical assistance and Dr. Qiudeng Que for critical reading of the manuscript. This study was funded by the USDA NIFA Biotechnology Risk Assessment Research Grant (2009-01067) to Z L. NR 51 TC 0 Z9 0 U1 1 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD SEP PY 2014 VL 12 IS 7 BP 951 EP 962 DI 10.1111/pbi.12203 PG 12 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA AN3ZS UT WOS:000340528000014 PM 24893677 ER PT J AU Park, M Han, J Ahn, Y Kim, J Lee, H Jang, Y Gaxiola, R Hirschi, KD Park, S AF Park, Mehea Han, Jeungsul Ahn, Yeulkyun Kim, Jeongho Lee, Hyeeun Jang, Yoonah Gaxiola, Roberto Hirschi, Kendal D. Park, Sunghun TI Ectopic expression of Arabidopsis H+-pyrophosphatase AVP1 enhances drought resistance in bottle gourd (Lagenaria siceraria Standl.) SO PLANT CELL TISSUE AND ORGAN CULTURE LA English DT Article DE Bottle gourd; Drought stress; H+-pyrophosphatase; Rootstock; Watermelon ID SALT TOLERANCE; IMPROVES; GENE; COTTON; PPASE; GROWTH AB Bottle gourd (Lagenaria sicerar ia Standl.) has been used as a source of rootstock for grafting watermelon to improve its fruit quality. We report here the development of a bottle gourd with resistance to drought by ectopic expression of the Arabidopsis AVP1 gene that encodes a vacuolar H+-pyrophosphatase. The drought resistance of AVP1-expressing and wild-type plants was assessed by growing plants under drought conditions. After 12 days of water deprivation, both AVP1-expressing and wild-type plants demonstrated reduced growth. After 10 days of re-watering, wild-type plants showed minimal growth while the AVP1-expressing plants resumed rapid growth. Further, AVP1-expressing plants displayed longer primary roots and more robust root systems than wild-type plants. C1 [Park, Mehea] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Postharvest Res Team, Suwon 440706, South Korea. [Han, Jeungsul] Kyungpook Natl Univ, Dept Ecol Environm Conservat, Sangju 742711, South Korea. [Ahn, Yeulkyun; Kim, Jeongho; Lee, Hyeeun; Jang, Yoonah] Rural Dev Adm, Natl Inst Hort & Herbal Sci, Vegetable Div, Suwon 440706, South Korea. [Gaxiola, Roberto] Arizona State Univ, Sch Life Sci SOLS, Tempe, AZ 85287 USA. [Hirschi, Kendal D.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Park, Sunghun] Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. RP Park, S (reprint author), Kansas State Univ, Dept Hort Forestry & Recreat Resources, Manhattan, KS 66506 USA. EM shpark@ksu.edu FU NIHHS RDA-KSU; RDA [PJJ00863802013] FX This research was supported by the NIHHS RDA-KSU Cooperative Research Project and a grant from the RDA (PJJ00863802013). NR 27 TC 1 Z9 1 U1 2 U2 28 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6857 EI 1573-5044 J9 PLANT CELL TISS ORG JI Plant Cell Tissue Organ Cult. PD SEP PY 2014 VL 118 IS 3 BP 383 EP 389 DI 10.1007/s11240-014-0490-8 PG 7 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA AN4NS UT WOS:000340565400002 ER PT J AU Moehninsi Miura, K Yamada, K Shigemori, H AF Moehninsi Miura, Kenji Yamada, Kosumi Shigemori, Hideyuki TI Raphanusanin-mediated resistance to pathogens is light dependent in radish and Arabidopsis thaliana SO PLANTA LA English DT Article DE Light-induced growth inhibitor; Light-mediated plant defense; Raphanusanin; Raphanusanin-mediated resistance ID PHOTOTROPISM-REGULATING SUBSTANCES; CUCUMBER MOSAIC-VIRUS; SALICYLIC-ACID; DETACHED-LEAF; PSEUDOMONAS-SYRINGAE; SIGNALING PATHWAYS; BOTRYTIS-CINEREA; INNATE IMMUNITY; MICROBIAL PATHOGENS; DEFENSE RESPONSES AB Raphanusanin (Ra) is a light-induced inhibitor of hypocotyl growth that responds to unilateral blue light illumination in radish seedlings. We have previously shown that Ra regulates genes that are involved in common defense mechanisms. Many genes that are induced by Ra are also positively regulated by early blue light. To extend the understanding of the role of Ra in pathogen defense, we evaluated the effects of Ra on radish and Arabidopsis thaliana (A. thaliana) infected with the necrotrophic pathogen Botrytis cinerea (B. cinerea) and biotrophic pathogen Pseudomonas syringae (P. syringae). Radish and A. thaliana were found to be resistant to both pathogens when treated with Ra, depending on the concentration used. Interestingly, Ra-mediated resistance to P. syringae is dependent on light because Ra-treated seedlings exhibited enhanced susceptibility to P. syringae infection when grown in the dark. In addition to regulating the biotic defense response, Ra inhibited seed germination and root elongation and enhanced the growth of root hairs in the presence of light in radish and A. thaliana. Our data suggest that Ra regulates the expression of a set of genes involved in defense signaling pathways and plays a role in pathogen defense and plant development. Our results show that light may be generally required not only for the accumulation of Ra but also for its activation during the pathogen defense response. C1 [Moehninsi; Miura, Kenji; Yamada, Kosumi; Shigemori, Hideyuki] Univ Tsukuba, Grad Sch Life & Environm Sci, Ibaraki 3058572, Japan. RP Moehninsi (reprint author), USDA ARS, Vegetable & Forage Crops Prod Res, Prosser, WA 99350 USA. EM moe.hninsi@ars.usda.gov; hshige@agbi.tsukuba.ac.jp RI Miura, Kenji/C-2803-2011 OI Miura, Kenji/0000-0003-1262-2176 FU Japanese Ministry of Education, Science, and Culture and Sports (Monbukagakusho) FX This work was supported by grants from Japanese Ministry of Education, Science, and Culture and Sports (Monbukagakusho). NR 66 TC 0 Z9 0 U1 1 U2 15 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0032-0935 EI 1432-2048 J9 PLANTA JI Planta PD SEP PY 2014 VL 240 IS 3 BP 513 EP 524 DI 10.1007/s00425-014-2103-5 PG 12 WC Plant Sciences SC Plant Sciences GA AN3PZ UT WOS:000340501800007 PM 24923677 ER PT J AU Arismendi, I Penaluna, BE Dunham, JB de Leaniz, CG Soto, D Fleming, IA Gomez-Uchida, D Gajardo, G Vargas, PV Leon-Munoz, J AF Arismendi, Ivan Penaluna, Brooke E. Dunham, Jason B. de Leaniz, Carlos Garcia Soto, Doris Fleming, Ian A. Gomez-Uchida, Daniel Gajardo, Gonzalo Vargas, Pamela V. Leon-Munoz, Jorge TI Differential invasion success of salmonids in southern Chile: patterns and hypotheses SO REVIEWS IN FISH BIOLOGY AND FISHERIES LA English DT Article DE Salmonids; Biological invasions; Propagule pressure; Environmental resistance; Biotic resistance; Non-native species; Chile ID FRESH-WATER FISHES; ONCORHYNCHUS-MYKISS WALBAUM; JUVENILE ATLANTIC SALMON; ZEALAND CHINOOK SALMON; EXOTIC PLANT INVASIONS; WESTERN NORTH-AMERICA; BROWN TROUT; RAINBOW-TROUT; LIFE-HISTORY; BIOLOGICAL INVASION AB Biological invasions create complex ecological and societal issues worldwide. Most of the knowledge about invasions comes only from successful invaders, but less is known about which processes determine the differential success of invasions. In this review, we develop a framework to identify the main dimensions driving the success and failure of invaders, including human influences, characteristics of the invader, and biotic interactions. We apply this framework by contrasting hypotheses and available evidence to explain variability in invasion success for 12 salmonids introduced to Chile. The success of Oncorhynchus mykiss and Salmo trutta seems to be influenced by a context-specific combination of their phenotypic plasticity, low ecosystem resistance, and propagule pressure. These well-established invaders may limit the success of subsequently introduced salmonids, with the possible exception of O. tshawytscha, which has a short freshwater residency and limited spatial overlap with trout. Although propagule pressure is high for O. kisutch and S. salar due to their intensive use in aquaculture, their lack of success in Chile may be explained by environmental resistance, including earlier spawning times than in their native ranges, and interactions with previously established and resident Rainbow Trout. Other salmonids have also failed to establish, and they exhibit a suite of ecological traits, environmental resistance, and limited propagule pressure that are variably associated with their lack of success. Collectively, understanding how the various drivers of invasion success interact may explain the differential success of invaders and provide key guidance for managing both positive and negative outcomes associated with their presence. C1 [Arismendi, Ivan] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Penaluna, Brooke E.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. [de Leaniz, Carlos Garcia] Swansea Univ, Dept Biosci, Swansea SA2 8PP, W Glam, Wales. [Soto, Doris] Food & Agr Org United Nations FAO, Aquaculture Branch, Dept Fisheries & Aquaculture, Rome, Italy. [Fleming, Ian A.] Mem Univ Newfoundland, Fish Evolutionary Ecol Res Grp, St John, NF A1C 5S7, Canada. [Fleming, Ian A.] Mem Univ Newfoundland, Dept Ocean Sci, St John, NF A1C 5S7, Canada. [Gomez-Uchida, Daniel; Vargas, Pamela V.] Univ Concepcion, Dept Zool, Interdisciplinary Ctr Aquaculture Res FONDAP INCA, Concepcion, Chile. [Gajardo, Gonzalo] Univ Los Lagos, Lab Genet Acuicultura & Biodiversidad, Osorno, Chile. [Leon-Munoz, Jorge] Minist Medio Ambiente, Secretaria Reg Minist Medio Ambiente Reg Rios, Valdivia, Chile. [Leon-Munoz, Jorge] Univ Austral Chile, Fac Ciencias, Inst Ciencias Marinas & Limnol, Valdivia, Chile. RP Arismendi, I (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. EM Ivan.Arismendi@oregonstate.edu RI Leon-Munoz, Jorge/K-8132-2014; Arismendi, Ivan/B-3144-2010; Fleming, Ian/I-7217-2012 OI Leon-Munoz, Jorge/0000-0002-5625-6721; Arismendi, Ivan/0000-0002-8774-9350; FU Fondo Nacional de Desarrollo Regional (FNDR Region de los Lagos); Fondo de Investigacion Pesquera of Chile FIP [2000-2024]; Comision Nacional de Ciencia y Tecnologia of Chile FONDECYT [1020183]; Millennium Nucleus Forest Ecosystem Services (FORECOS) Ministerio de Planificacion of Chile [P04-065-F]; Department for Environment Food and Rural Affairs (DEFRA, UK) Darwin Initiative [162-15-020, EIDPOC 041]; Fondo de Financiamiento de Centros de Excelencia en Investigacion FONDAP [15110027] FX Cristian Correa, four anonymous reviewers and the Associated Editor William Ardren provided comments that improved the manuscript. Funded by Fondo Nacional de Desarrollo Regional (FNDR Region de los Lagos); Fondo de Investigacion Pesquera of Chile FIP 2000-2024; Comision Nacional de Ciencia y Tecnologia of Chile FONDECYT Grant 1020183; Millennium Nucleus Forest Ecosystem Services (FORECOS) P04-065-F Ministerio de Planificacion of Chile; and, Department for Environment Food and Rural Affairs (DEFRA, UK) Darwin Initiative Grants # 162-15-020 and EIDPOC 041. Fondo de Financiamiento de Centros de Excelencia en Investigacion FONDAP 15110027. Carlos Jara, Antonio Lara, Stefan Woelfl, Guillermo Giannico, and Jose Luis Iriarte provided comments on an early version of the manuscript. Tiffany Garcia and Kate Boersma provided comments on the conceptual framework figure and Kathryn Ronnenberg helped with its design. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US Government. NR 199 TC 13 Z9 13 U1 2 U2 44 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3166 EI 1573-5184 J9 REV FISH BIOL FISHER JI Rev. Fish. Biol. Fish. PD SEP PY 2014 VL 24 IS 3 SI SI BP 919 EP 941 DI 10.1007/s11160-014-9351-0 PG 23 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AN2ZS UT WOS:000340455200013 ER PT J AU Jane, SF Nislow, KH Whiteley, AR AF Jane, Stephen F. Nislow, Keith H. Whiteley, Andrew R. TI The use (and misuse) of archaeological salmon data to infer historical abundance in North America with a focus on New England SO REVIEWS IN FISH BIOLOGY AND FISHERIES LA English DT Article DE Archaeological; Calcined; Middens; Bone; Conservation decision making; Historical abundance; Pacific salmon; Atlantic salmon ID ATLANTIC SALMON; NATIVE RANGE; SALAR; MIGRATION; PACIFIC; TROUT; TEMPERATURE; TRUTTA; DAMS; BONE AB Information about historical animal or plant abundance often either explicitly or implicitly informs current conservation practice. If it can be shown that an organism was not historically abundant in a region, its conservation importance may be downgraded. In contrast to abundant archaeological support for historic importance of salmon in the Pacific Northwest, historic abundance of Atlantic salmon in New England has been called into question based on the rarity of salmon bones in archaeological sites. These data have been used to argue that the importance of salmon to the region has been exaggerated and that expensive restoration efforts in some rivers should be reconsidered. Here, we argue that lack of archaeological bone fragment abundance does not make a convincing case against historical Atlantic salmon abundance in New England for three primary reasons. First, salmon bones were rare or absent at sites that still have large salmon runs. Second, the lack of salmonid bones in general at archaeological sites suggests poor preservation and/or recovery of bone for these species relative to bones of other fishes. Third, given the presence of large numbers of non-salmonid anadromous fish in the site areas where people fished and deposited fish bones, power to detect salmon bones in studies to date may have been generally low. We present reliable historical accounts that help build a convincing case that salmon were historically abundant in New England rivers. We suggest that rarity of salmon bones in the existing archaeological data should not have unwarranted influence on present-day conservation decision-making in New England. C1 [Jane, Stephen F.; Whiteley, Andrew R.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Nislow, Keith H.] Univ Massachusetts, No Res Stn, US Forest Serv, Amherst, MA 01003 USA. RP Jane, SF (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. EM coachman7777@yahoo.com NR 56 TC 2 Z9 2 U1 3 U2 15 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3166 EI 1573-5184 J9 REV FISH BIOL FISHER JI Rev. Fish. Biol. Fish. PD SEP PY 2014 VL 24 IS 3 SI SI BP 943 EP 954 DI 10.1007/s11160-013-9337-3 PG 12 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AN2ZS UT WOS:000340455200014 ER PT J AU Liu, ZJ Fei, BH Jiang, ZH Cai, ZY Liu, XE AF Liu, Zhijia Fei, Benhua Jiang, Zehui Cai, Zhiyong Liu, Xing'e TI Important properties of bamboo pellets to be used as commercial solid fuel in China SO WOOD SCIENCE AND TECHNOLOGY LA English DT Article ID COMBUSTION CHARACTERISTICS; BIOMASS FUELS; BEHAVIOR; ENERGY; WOOD AB Bamboo is a type of biomass material and has great potential as a bio-energy resource of the future in China. Some properties of bamboo pellets, length, diameter, moisture content (MC), particle density, bulk density, durability, fine content, ash, gross calorific value, combustion rate and heat release rate, were determined and the effects of MC and particle size (PS) on these properties were investigated in this research. The results showed that bamboo pellets will be the proposed new biomass solid fuel and have the potential to be developed as commercial pellets. All properties of bamboo pellets met the requirement of Pellet Fuels Institute Standard Specification for Residential/Commercial Densified Fuel. The gross calorific value of bamboo pellets also met the minimum requirement for making commercial pellets according to DIN 51731 (> 17,500 J/g). The effects of MC on length, diameter, MC, particle density and bulk density were significant at p = 0.05. There were no significant differences between PS and all properties of bamboo pellets. Due to higher MC and larger PS, bamboo pellets exhibited higher combustion rates and heat release rates. This research represents an initial stage in the study of bamboo pellets, and its objectives provide guidelines for further research. C1 [Liu, Zhijia; Fei, Benhua; Jiang, Zehui; Liu, Xing'e] Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. [Cai, Zhiyong] US Forest Serv, Forest Prod Lab, USDA, Madison, WI 53705 USA. RP Liu, ZJ (reprint author), Int Ctr Bamboo & Rattan, Beijing 100102, Peoples R China. EM liuzj@icbr.ac.cn FU 12th Five Years Plan-Study on manufacturing technology of functional bamboo (rattan)-based materials [2012BAD54G01]; Basic Scientific Research Funds of International Centre for Bamboo and Rattan [1632012002] FX This research was financially supported by '12th Five Years Plan-Study on manufacturing technology of functional bamboo (rattan)-based materials' (Grant No. 2012BAD54G01) and 'Basic Scientific Research Funds of International Centre for Bamboo and Rattan' (Grant No. 1632012002). NR 22 TC 2 Z9 2 U1 4 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0043-7719 EI 1432-5225 J9 WOOD SCI TECHNOL JI Wood Sci. Technol. PD SEP PY 2014 VL 48 IS 5 BP 903 EP 917 DI 10.1007/s00226-014-0648-x PG 15 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA AN4FA UT WOS:000340541800003 ER PT J AU Karl, JP Cheatham, RA Das, SK Hyatt, RR Gilhooly, CH Pittas, AG Lieberman, HR Lerner, D Roberts, SB Saltzman, E AF Karl, J. Philip Cheatham, Rachel A. Das, Sai Krupa Hyatt, Raymond R. Gilhooly, Cheryl H. Pittas, Anastassios G. Lieberman, Harris R. Lerner, Debra Roberts, Susan B. Saltzman, Edward TI Effect of glycemic load on eating behavior self-efficacy during weight loss SO APPETITE LA English DT Article DE Energy restriction; Glycemic index; Weight maintenance; Weight regain; Weight self-efficacy ID DOUBLY LABELED WATER; MIDDLE-AGED WOMEN; LOSS MAINTENANCE; OBESE WOMEN; ENERGY-EXPENDITURE; DIETARY ADHERENCE; OVERWEIGHT MEN; PREDICTORS; HEALTH; REDUCTION AB High eating behavior self-efficacy may contribute to successful weight loss. Diet interventions that maximize eating behavior self-efficacy may therefore improve weight loss outcomes. However, data on the effect of diet composition on eating behavior self-efficacy are sparse. To determine the effects of dietary glycemic load (GL) on eating behavior self-efficacy during weight loss, body weight and eating behavior self-efficacy were measured every six months in overweight adults participating in a 12-mo randomized trial testing energy-restricted diets differing in GL. All food was provided during the first six months and self-selected thereafter. Total mean weight loss did not differ between groups, and GL-level had no significant effect on eating behavior self-efficacy. In the combined cohort, individuals losing the most weight reported improvements in eating behavior self-efficacy, whereas those achieving less weight loss reported decrements in eating behavior self-efficacy. Decrements in eating behavior self-efficacy were associated with subsequent weight regain when diets were self-selected. While GL does not appear to influence eating behavior self-efficacy, lesser amounts of weight loss on provided-food energy restricted diets may deter successful maintenance of weight loss by attenuating improvements in eating behavior self-efficacy. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Karl, J. Philip; Cheatham, Rachel A.; Das, Sai Krupa; Hyatt, Raymond R.; Gilhooly, Cheryl H.; Roberts, Susan B.; Saltzman, Edward] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Pittas, Anastassios G.] Tufts Med Ctr, Div Endocrinol Diabet & Metab, Boston, MA 02111 USA. [Lieberman, Harris R.] US Army Res Inst Environm Med, Mil Nutr Div, Natick, MA 01760 USA. [Lerner, Debra] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Boston, MA 02111 USA. RP Saltzman, E (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM edward.saltzman@tufts.edu RI Biguzzi, Felipe/E-4724-2015; OI Karl, J. Philip/0000-0002-5871-2241 FU U.S. Department of Agriculture, Agricultural Research Service [58-1950-7-707]; National Institutes of Health [U01-AG20480]; U.S. Department of Agriculture from the National Institutes of Diabetes and Digestive and Kidney Diseases [58-1950-4-401, K23 DK61506]; Boston Nutrition Obesity Research Center (BONRC) [H150001]; Science, Mathematics, and Research Transformation Defense Education Program; NIH [DK62032-11] FX The study is based upon work supported by the U.S. Department of Agriculture, Agricultural Research Service, under agreement No. 58-1950-7-707. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the view of the U.S. Department of Agriculture, the U.S. Army, or the U.S. Department of Defense. The Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy trial-phase I study was supported by National Institutes of Health grant U01-AG20480, the U.S. Department of Agriculture under agreement No. 58-1950-4-401, K23 DK61506 from the National Institutes of Diabetes and Digestive and Kidney Diseases, and Boston Nutrition Obesity Research Center (BONRC) H150001. JPK was supported by the Science, Mathematics, and Research Transformation Defense Education Program. RAC was supported by a NIH T32 grant (#DK62032-11). We wish to thank the study volunteers and the staff of the Metabolic Research Unit for their dedication to this project. U.S. National Institutes of Health clinicaltrials.gov identifier: NCT00099099. Conflict of interest: No authors report a conflict of interest. NR 47 TC 1 Z9 1 U1 1 U2 13 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0195-6663 EI 1095-8304 J9 APPETITE JI Appetite PD SEP 1 PY 2014 VL 80 BP 204 EP 211 DI 10.1016/j.appet.2014.05.017 PG 8 WC Behavioral Sciences; Nutrition & Dietetics SC Behavioral Sciences; Nutrition & Dietetics GA AN0XS UT WOS:000340307900028 PM 24859114 ER PT J AU Bell, MD Sickman, JO Bytnerowicz, A Padgett, PE Allen, EB AF Bell, Michael D. Sickman, James O. Bytnerowicz, Andrzej Padgett, Pamela E. Allen, Edith B. TI Variation in isotopologues of atmospheric nitric acid in passively collected samples along an air pollution gradient in southern California SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Nylasorb nylon filter; Atmospheric deposition; Passive sampler; Nitric acid; delta N-15; delta O-18; Lightning ID ISOTOPIC COMPOSITION; NITROGEN DEPOSITION; DRY DEPOSITION; REACTIVE NITROGEN; NITRATE; OXYGEN; FRACTIONATION; TROPOSPHERE; AMMONIA; OXIDES AB The sources and oxidation pathways of atmospheric nitric acid (HNO3) can be evaluated using the isotopic signatures of oxygen (O) and nitrogen (N). This study evaluated the ability of Nylasorb nylon filters to passively collect unbiased isotopologues of atmospheric HNO3 under controlled and field conditions. Filters contained in passive samplers were exposed in continuous stirred tank reactors (CSTRs) at high (16 mu g/m(3)) and moderate (8 mu g/m(3)) HNO3 concentrations during 1-4 week deployment times. Filters were concurrently exposed at high and low N deposition sites along a gradient in the Sonoran Desert. Filters deployed in CSTRs at moderate HNO3 concentrations for 1-2 weeks had greater variation of delta O-18 relative to the 3-4 week deployments, while high concentration samples were consistent across weeks. All deployment means were within 2 parts per thousand of the source solution. The delta N-18 of all weekly samples were within 0.5 parts per thousand of the source solution. Thus, when deployed for longer than 3 weeks, Nylasorb filters collected an isotopically unbiased sample of atmospheric HNO3. The initial HNO3 samples at the high deposition field sites had higher delta N-18 and delta O-18 values than the low deposition sites, suggesting either two independent sources of HNO3 were mixing or that heavier isotopologues of HNO3 were preferentially lost from the gas phase through physical deposition or equilibrium chemical reactions. Subsequent HNO3 samples were subject to summer monsoon conditions leading to variation of isotopic signatures of N and 0 following 2-source mixing dynamics. Both sites mixed with a source that dominated during the two discrete precipitation events. The high number of lightning strikes near the samplers during the monsoon events suggested that lightning-created HNO3 was one of the dominant mixing sources with an approximate isotopic signature of 21.6 parts per thousand and 0.6 parts per thousand for delta O-18 and delta N-18 respectively. Two-source mixing models suggest that lightning-created HNO3 made up between 40 and 42% of atmospheric HNO3 at the high deposition sites and 59-63% at the low deposition during the 4 week exposure. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Bell, Michael D.; Allen, Edith B.] Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA. [Sickman, James O.] Univ Calif Irvine, Dept Environm Sci, Irvine, CA 92697 USA. [Bytnerowicz, Andrzej; Padgett, Pamela E.] US Forest Serv, USDA, Pacific Southwest Res Stn, Riverside, CA 92507 USA. RP Bell, MD (reprint author), Univ Calif Irvine, Ctr Environm Biol, 243 Steinhaus Hall, Irvine, CA 92697 USA. EM bellmd@uci.edu FU National Science Foundation [DEB-1110832]; San Bernardino Community Foundation Desert Research Fund FX This research was made possible by grants from the National Science Foundation (DEB-1110832) and the San Bernardino Community Foundation Desert Research Fund. The authors would like to thank Josh Hoines and Katie Kain from Joshua Tree National Park and Katie O'Conner from the Friends of the Desert Mountains for assistance with site access and Delores Lucero, Diane Alexander, Elizabeth Hessom, Ben Wissinger, and Taahira Major for field and lab assistance. We would also like to thank the two anonymous reviewers for their comments on the initial submission. NR 45 TC 1 Z9 1 U1 3 U2 19 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD SEP PY 2014 VL 94 BP 287 EP 296 DI 10.1016/j.atmosenv.2014.05.031 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AN1AY UT WOS:000340316300032 ER PT J AU Suckling, DM Stringer, LD Baird, DB Butler, RC Sullivan, TES Lance, DR Simmons, GS AF Suckling, D. M. Stringer, L. D. Baird, D. B. Butler, R. C. Sullivan, T. E. S. Lance, D. R. Simmons, G. S. TI Light brown apple moth (Epiphyas postvittana) (Lepidoptera: Tortricidae) colonization of California SO BIOLOGICAL INVASIONS LA English DT Article DE Invasion; Incursion; Pheromone trap; Population spread; Delimitation; Leafroller ID SEX-PHEROMONE BLEND; NEW-ZEALAND; PLANOTORTRIX-OCTO; GYPSY-MOTH; TRAPS; WALKER; IDENTIFICATION; MANAGEMENT; NOCTUIDAE; ECOLOGY AB In response to the confirmed detection of the light brown apple moth, Epiphyas postvittana, in California, approximately 53,000 pheromone-baited Jackson traps were deployed and more than 246,000 males were caught (February 2007-February 2010). Approximately 46,000 manually entered catch records were corrected for errors and converted into catch per trap per day. As empty trap data (zeros) were not recorded, we added zeros between first and last catch for each trap based on the stated servicing period (similar to 30,000), before analyzing for trends. Residual data error rate was estimated as 1.5 %. San Francisco and Santa Cruz counties had relatively high trap catches immediately upon trap set, and remained the leading population centers, while most other counties showed a more general trend of a slow build-up in catch over time (12 counties). An exponential increase in trap catch was observed in four counties with sufficient data. The pattern of spread indicated natural, as well as anthropogenic-assisted spread rates, with populations appearing well ahead of the invasion front. This jump dispersal is probably due to movement of host plants, unsurprising since eggs of this polyphagous moth are readily laid on foliage. There was evidence of seasonality in spread, probably linked to the phenology of the insect. There was a positive relationship between catch and known host tree preference, suggesting that trap placement in preferred hosts could add sensitivity to future surveys. Recommendations include the improved provision of data acquisition by telecommunications, standardization of data input, more archiving, and frequent analysis of trap catches. The rapid rate of population growth demonstrated in two counties and spread across many others supports the hypothesis of the recent arrival of E. postvittana in California. C1 [Suckling, D. M.; Stringer, L. D.; Butler, R. C.; Sullivan, T. E. S.] New Zealand Inst Plant & Food Res Ltd, Christchurch 8140, New Zealand. [Lance, D. R.] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Simmons, G. S.] USDA APHIS PPQ CPHST, Moss Landing, CA 95039 USA. RP Suckling, DM (reprint author), New Zealand Inst Plant & Food Res Ltd, PB 4704, Christchurch 8140, New Zealand. EM max.suckling@plantandfood.co.nz RI Butler, Ruth/D-5825-2011; Suckling, David/F-7005-2010; OI Butler, Ruth/0000-0001-8646-1147; Suckling, David/0000-0001-7216-9348; Stringer, Lloyd/0000-0002-6345-0768 FU US Department of Agriculture; New Zealand Ministry for Science and Innovation (Better Border Biosecurity); Organisation for Economic Cooperation and Development (Cooperative Research Programme: Biological Resource Management for Sustainable Agricultural Systems); European Biological Control Laboratory (USDA-Agricultural Research Service), Montpellier, France FX This work was funded by the US Department of Agriculture and the New Zealand Ministry for Science and Innovation (Better Border Biosecurity, www.b3nz.org) and completed during a fellowship to DMS supported by the Organisation for Economic Cooperation and Development (Cooperative Research Programme: Biological Resource Management for Sustainable Agricultural Systems) at the European Biological Control Laboratory (USDA-Agricultural Research Service), Montpellier, France. We thank Professor Jerry Powell for providing light trap data on light brown apple moth and the California Department of Food and Agriculture for their cooperation. We thank Kathleen Harding, Laura Irons and Tim Horton for data mining and providing information on trapping operations. NR 42 TC 9 Z9 9 U1 4 U2 19 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD SEP PY 2014 VL 16 IS 9 BP 1851 EP 1863 DI 10.1007/s10530-013-0631-8 PG 13 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM6JH UT WOS:000339970100006 ER PT J AU Spellman, KV Mulder, CPH Hollingsworth, TN AF Spellman, Katie V. Mulder, Christa P. H. Hollingsworth, Teresa N. TI Susceptibility of burned black spruce (Picea mariana) forests to non-native plant invasions in interior Alaska SO BIOLOGICAL INVASIONS LA English DT Article DE Boreal forest; Bromus inermis ssp inermis; Climate change; Disturbance; Fire; Hieracium aurantiacum; Melilotus albus; Wildfire ID BOREAL FOREST; NATIONAL-PARK; HIGH-LATITUDES; CLIMATE-CHANGE; FIRE SEVERITY; ECOSYSTEMS; SUCCESSION; COMMUNITIES; BIODIVERSITY; INVASIBILITY AB As climate rapidly warms at high-latitudes, the boreal forest faces the simultaneous threats of increasing invasive plant abundances and increasing area burned by wildfire. Highly flammable and widespread black spruce (Picea mariana) forest represents a boreal habitat that may be increasingly susceptible to non-native plant invasion. This study assess the role of burn severity, site moisture and time elapsed since burning in determining the invisibility of black spruce forests. We conducted field surveys for presence of non-native plants at 99 burned black spruce forest sites burned in 2004 in three regions of interior Alaska that spanned a gradient of burn severities and site moisture levels, and a chronosequence of sites in a single region that had burned in 1987, 1994, and 1999. We also conducted a greenhouse experiment where we grew invasive plants in vegetation and soil cores taken from a subset of these sites. In both our field survey and the greenhouse experiment, regional differences in soils and vegetation between burn complexes outweighed local burn severity or site moisture in determining the invasibility of burned black spruce sites. In the greenhouse experiments using cores from the 2004 burns, we found that the invasive focal species grew better in cores with soil and vegetation properties characteristic of low severity burns. Invasive plant growth in the greenhouse was greater in cores from the chronosequence burns with higher soil water holding capacity or lower native vascular biomass. We concluded that there are differences in susceptibility to non-native plant invasions between different regions of boreal Alaska based on native species regeneration. Re-establishment of native ground cover vegetation, including rapidly colonizing bryophytes, appear to offer burned areas a level of resistance to invasive plant establishment. C1 [Spellman, Katie V.; Mulder, Christa P. H.] Univ Alaska Fairbanks, Inst Arctic Biol, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. [Hollingsworth, Teresa N.] US Forest Serv, Pacific NW Res Stn, Boreal Ecol Cooperat Res Unit, Fairbanks, AK 99775 USA. RP Spellman, KV (reprint author), Univ Alaska Fairbanks, Inst Arctic Biol, Dept Biol & Wildlife, Fairbanks, AK 99775 USA. EM katie.spellman@alaska.edu FU Center for Global Change and Arctic System Research; Center for Invasive Plant Management; Arctic Audubon Society FX We thank M. L. Carlson, B. T. Spellman, and four anonymous reviewers for their comments, and J. F. Johnstone and F. S. Chapin III for helping with conceptual development of the project. We thank the family and friends who volunteered to help in the field and greenhouse components of this project. H. McIntyre, J. Martin, and the staff of the University of Alaska Fairbanks Institute of Arctic Biology Greenhouse provided support for the greenhouse study. The map for Fig. 1 was created by Jaime Hollingsworth. Logistical support for our project was provided by the University of Alaska Fairbanks Institute of Arctic Biology and Bonanza Creek Long Term Ecological Research Program. Funding for this project was provided by grants from the Center for Global Change and Arctic System Research, the Center for Invasive Plant Management, and the Arctic Audubon Society. NR 59 TC 3 Z9 3 U1 5 U2 51 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD SEP PY 2014 VL 16 IS 9 BP 1879 EP 1895 DI 10.1007/s10530-013-0633-6 PG 17 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM6JH UT WOS:000339970100008 ER PT J AU Shiels, AB Ennis, MK Shiels, L AF Shiels, Aaron B. Ennis, Martin K. Shiels, Laura TI Trait-based plant mortality and preference for native versus non-native seedlings by invasive slug and snail herbivores in Hawaii SO BIOLOGICAL INVASIONS LA English DT Article DE Achatina fulica; Enemy release hypothesis; Forest community structure; Hawaiian Islands; Veronicella cubensis; Woody seedling herbivory ID ENEMY RELEASE HYPOTHESIS; GRASSLAND; VEGETATION; WOODLAND; IMPACTS; MOLLUSK; FORESTS; THREAT AB Non-native herbivores may alter plant communities through their preferential consumption of seedlings of different species. We assessed seedling herbivory by two invasive gastropod species in Hawaii, the giant African snail (Achatina fulica) and the Cuban brown slug (Veronicella cubensis). We hypothesized that six native species would suffer greater gastropod herbivory than four non-native species, and that species with short stature, thin leaves, and lacking physical defenses would suffer the greatest mortality from gastropods. Herbivory was measured during 13-day preference trials using enclosures that each contained four different woody species (two native, two non-native) and were assigned to one of three treatments: giant African snail, Cuban brown slug, or control (no gastropod). Discriminant function analysis was used to predict gastropod-induced seedling mortality from a suite of seedling characteristics. Native species did not always experience greater herbivory than non-natives species, and seedling mortality was 0-100 %. Native Pipturus albidus and Clermontia parviflora suffered 100 % mortality from V. cubensis herbivory, and P. albidus, Psychotria hawaiiensis, and Myrsine lessertiana suffered a parts per thousand yen80 % mortality from A. fulica. Two non-natives (Fraxinus uhdei, Clidemia hirta), and two natives (Metrosideros polymorpha, Diospyros sandwicensis), suffered little damage and no mortality. Non-native Ardisia elliptica suffered 10-30 % gastropod mortality, and non-native Psidium cattleianum mortality was 0-50 %. Leaf thickness best predicted species mortality caused by slugs and snails; some thicker-leaved species suffered most. Invasive snails and slugs threaten some native and non-native seedlings by directly consuming them. Current and future plant community structure in Hawaii may in part reflect the feeding preferences of invasive gastropods. C1 [Shiels, Aaron B.] USDA, APHIS, Natl Wildlife Res Ctr, Hawaii Field Stn, Hilo, HI 96721 USA. [Ennis, Martin K.] Univ Hawaii, Coll Agr Forestry & Nat Resource Management, Hilo, HI 96720 USA. [Shiels, Laura] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. RP Shiels, AB (reprint author), USDA, APHIS, Natl Wildlife Res Ctr, Hawaii Field Stn, POB 10880, Hilo, HI 96721 USA. EM ashiels@hawaii.edu NR 39 TC 2 Z9 2 U1 10 U2 73 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD SEP PY 2014 VL 16 IS 9 BP 1929 EP 1940 DI 10.1007/s10530-013-0636-3 PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM6JH UT WOS:000339970100011 ER PT J AU West, NM Matlaga, DP Davis, AS AF West, Natalie M. Matlaga, David P. Davis, Adam S. TI Quantifying targets to manage invasion risk: light gradients dominate the early regeneration niche of naturalized and pre-commercial Miscanthus populations SO BIOLOGICAL INVASIONS LA English DT Article DE Miscanthus; Biofuels; Regeneration niche; Cultivation; Response surface ID EXOTIC PLANT INVASION; X-GIGANTEUS; UNITED-STATES; SINENSIS; RECRUITMENT; SEEDLINGS; LITTER; PENETRATION; COMMUNITIES; GERMINATION AB Prospective bioenergy crops have caused concern about their invasive potential because they often share characteristics with known invasive species. Studies that examine the factors that limit regeneration of these crop species will be crucial for identifying vulnerable habitats and devising management strategies to reduce the likelihood of escape from cultivation. Using a response surface design, we investigated the influence of light availability, soil moisture, and litter cover on recruitment and establishment of a potential biofuel cultivar of Miscanthus as well as an invasive congener. Responses were similar for the two plant types. Light availability had a strong influence on seedling success at both stages, though light limitation prevented establishment only at the lowest light level. Although variation in recruitment rates was low within plant types, establishment varied extensively in response to different light conditions. Low variation in Miscanthus seedling recruitment that led to establishment of a seedling bank under a range of light intensities may facilitate a "sit and wait" situation that raises the likelihood of successful escapes. Therefore, management efforts that restrict seed movement and increase light competition for seedlings will be important for lowering invasion risk. As deliberate introductions of bioenergy crops increase, ecological studies that quantify conditions required for successful escapes will be key to helping agronomists and managers mitigate the risk of unintended invasions. C1 [West, Natalie M.; Matlaga, David P.; Davis, Adam S.] USDA ARS, Global Change & Photosynth Res Unit, Urbana, IL 61801 USA. [Matlaga, David P.] Susquehanna Univ, Dept Biol, Selinsgrove, PA 17870 USA. RP West, NM (reprint author), USDA ARS, Global Change & Photosynth Res Unit, 1102 S Goodwin Ave, Urbana, IL 61801 USA. EM nmwest@illinois.edu FU USDA NIFA AFRI [2012-67013-19427] FX We would like to thank C. Czekala, V. Fillicetti, and T. Rewal for help with data collection, and J. Moody and the University of Illinois Plant Care Facility for help with logistics and experimental maintenance. We would also like to thank Mendel Biotechnology for sharing pre-commercial materials used in the experiment, and particularly D. Allen for facilitating efforts and answering questions. This work was funded by USDA NIFA AFRI Project #2012-67013-19427. NR 49 TC 6 Z9 6 U1 1 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1387-3547 EI 1573-1464 J9 BIOL INVASIONS JI Biol. Invasions PD SEP PY 2014 VL 16 IS 9 BP 1991 EP 2001 DI 10.1007/s10530-014-0643-z PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM6JH UT WOS:000339970100016 ER PT J AU Cleveland, BM AF Cleveland, Beth M. TI In vitro and in vivo effects of phytoestrogens on protein turnover in rainbow trout (Oncorhynchus mykiss) white muscle SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY LA English DT Article DE Proteolysis; Atrogin-1; Genistein; Fish; fbxo32 ID STURGEON ACIPENSER-BAERI; GROWTH-FACTOR-I; ESTROGEN RESPONSE ELEMENTS; SIBERIAN STURGEON; SOY PROTEIN; FISH-MEAL; ISOFLAVONES GENISTEIN; SARCOMERIC PROTEINS; DIETARY ISOFLAVONES; UBIQUITIN LIGASE AB Soybeans and other legumes investigated as fishmeal replacements in aquafeeds contain phytoestrogens capable of binding to and activating estrogen receptors. Estradiol has catabolic effects in salmonid white muscle, partially through increases in protein turnover. The current study determines whether phytoestrogens promote similar effects. In rainbow trout (Oncorhynchus mykiss) primary myocyte cultures, the phytoestrogens genistein, daidzein, glycitein, and R- and S-equol reduced rates of protein synthesis and genistein, the phytoestrogen of greatest abundance in soy, also increased rates of protein degradation. Increased expression of the ubiquitin ligase fbxo32 and autophagy-related genes was observed with high concentrations of genistein (100 mu M), and R- and S-equol (100 mu M) also up-regulated autophagy-related genes. In contrast, low genistein concentrations in vitro (0.01-0.10 mu M) and in vivo (5 mu g/g body mass) decreased fbxo32 expression, suggesting a potential metabolic benefit for low levels of genistein exposure. Phytoestrogens reduced cell proliferation, indicating that effects of phytoestrogens extend from metabolic to mitogenic processes. Co-incubation of genistein with the estrogen receptor (ER) antagonist, ICI 182,780, ameliorated effects of genistein on protein degradation, but not protein synthesis or cell proliferation, indicating that effects of genistein are mediated through ER-dependent and ER-independent mechanisms. Collectively, these data warrant additional studies to determine the extent to which dietary phytoestrogens, especially genistein, affect physiological processes that impact growth and nutrient retention. Published by Elsevier Inc. C1 [Cleveland, Beth M.] ARS, Natl Ctr Cool & Cold Water Aquaculture, USDA, Kearneysville, WV 25430 USA. RP Cleveland, BM (reprint author), NCCCWA, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM beth.cleveland@ars.usda.gov FU Agricultural Research Service Project [1930-31000-010-000D] FX The author is infinitely grateful to Lisa Radler for her valuable and essential technical and intellectual contributions. The technical and animal caretaking contributions from Josh Kretzer, Jenea McGowan, and Kyle Jenkins are acknowledged. Mention of trade names is solely for the purpose of providing accurate information and should not imply product endorsement by the United States Department of Agriculture. USDA is an equal opportunity provider and employer. Funding for this study came from the Agricultural Research Service Project1930-31000-010-000D. NR 55 TC 3 Z9 3 U1 3 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1532-0456 EI 1878-1659 J9 COMP BIOCHEM PHYS C JI Comp. Biochem. Physiol. C-Toxicol. Pharmacol. PD SEP PY 2014 VL 165 BP 9 EP 16 DI 10.1016/j.cbpc.2014.05.003 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology SC Biochemistry & Molecular Biology; Endocrinology & Metabolism; Toxicology; Zoology GA AM9SZ UT WOS:000340222600002 PM 24874080 ER PT J AU Accinelli, C Abbas, HK Vicari, A Shier, WT AF Accinelli, Cesare Abbas, Hamed K. Vicari, Alberto Shier, W. Thomas TI Aflatoxin contamination of corn under different agro-environmental conditions and biocontrol applications SO CROP PROTECTION LA English DT Article DE Aflatoxin; Aspergillus flavus; Bioplastic; Biopesticides; Zea mays; Bt corn ID ASPERGILLUS-FLAVUS; BIOLOGICAL-CONTROL; FIELD APPLICATION; UNITED-STATES; MYCOTOXIN; CROPS; BIOSYNTHESIS; POPULATIONS; COTTONSEED; REDUCTION AB Biological control of the fungus Aspergillus flavus has been shown to be effective in reducing aflatoxin contamination in corn. This study compared field application of a bioplastic-based formulation for delivering atoxigenic A. flavus isolates in Northern Italy and the Mississippi Delta. Due to an extremely hot and dry summer at the Italy site in 2012, aflatoxin contamination was approximately seven times higher than in 2011. In 2011, and 2012, application of bioplastic granules inoculated with the atoxigenic isolate A. flavus NRRL 30797 at 15 and 30 kg ha(-1) resulted in a reduction of aflatoxin contamination by 67.2 +/- 4.1% and 94.8 +/- 5.3%, respectively. The higher application rate was also effective when soil abundance of A. flavus was artificially increased by applying contaminated corn residues. At the Mississippi site, summer 2012 was also hot and dry, with high levels of aflatoxin contamination. In fields planted with non-Bt or Bt hybrids, application of biocontrol granules inoculated with A. flavus NRRL 30797 or NRRL 21882 at 30 kg ha(-1) reduced aflatoxin contamination to up to 89.6%. Field experiments on two continents showed that bioplastic-based A. flavus formulations markedly reduced aflatoxin contamination under different agro-environmental conditions and infestation intensities. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Accinelli, Cesare; Vicari, Alberto] Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. [Abbas, Hamed K.] USDA ARS, Biol Control Pests Res Unit, Stoneville, MS 38776 USA. [Shier, W. Thomas] Univ Minnesota, Coll Pharm, Dept Med Chem, Minneapolis, MN 55455 USA. RP Accinelli, C (reprint author), Univ Bologna, Dept Agr Sci, I-40127 Bologna, Italy. EM cesare.accinelli@unibo.it OI Accinelli, Cesare/0000-0001-8200-0108 FU Syngenta Crop Protection [58-6402-0-500] FX We would like to thank Alemah Butler, Jeremy Kotowicz, Roderick Patterson, and Terry Johnson for their technical assistance. This research was partially funded by Syngenta Crop Protection under Specific Cooperative Agreement no. 58-6402-0-500. NR 29 TC 5 Z9 5 U1 0 U2 27 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD SEP PY 2014 VL 63 BP 9 EP 14 DI 10.1016/j.cropro.2014.04.021 PG 6 WC Agronomy SC Agriculture GA AN1AE UT WOS:000340314300002 ER PT J AU Leesch, JG Smilanick, JL Muhareb, JS Tebbets, JS Hurley, JM Jones, TM AF Leesch, J. G. Smilanick, J. L. Muhareb, J. S. Tebbets, J. S. Hurley, J. M. Jones, T. M. TI Effects of box liner perforation area on methyl bromide diffusion into table grape packages during fumigation SO CROP PROTECTION LA English DT Article DE Grapes; Methyl bromide; Liners; Perforation; Diffusion; Residues ID SULFUR-DIOXIDE; RESIDUES; FRUIT; PERMEABILITY; SORPTION; FILMS AB Plastic liners are used inside boxes of table grapes to retard moisture loss from the grapes and to contain sulfur dioxide gas released inside the packages to control postharvest decay. However, to control organisms of quarantine concern, regulators specify exported packages must be fumigated with methyl bromide (MB), and to enable adequate diffusion of the fumigant into the packages they specify the liners must be perforated. The percentage of the area of the liner that is perforated, formerly stipulated to be not less than 0.3%, was recently increased to not less than 0.9%. Two MB fumigation schedules specified for control of the Chilean mite, Brevipalpus chilensis, were applied to grape packages with a high-density polyethylene liners with perforated areas of 0.9% or with a SO2-releasing liners with perforated areas of 0.3, 0.6, or 0.9%. Package and chamber concentrations were measured repeatedly for up to three hours during MB fumigation at 4.4 or 6.0 degrees C with a dosage 64 mg L-1 or at 26.7 degrees C with a dosage 56 mg L-1. Diffusion was similar and rapid into the packages among all perforated areas. MB concentrations inside the packages were not less than 95% of those of the chamber atmosphere within 15 min. After fumigation with an MB dosage 64 mg L-1 at 4.4 degrees C and subsequent storage at 2.0 degrees C, mean MB residue content in grapes from most packages 48 h after MB fumigation was below the limit of quantitation of 0.002 mg kg(-1). After fumigation with an MB dosage 56 mg L-1 at 26.7 degrees C and subsequent storage at 2.0 degrees C, mean MB residue content in grapes from most packages 24 h after MB fumigation was below the limit of quantitation. Published by Elsevier Ltd. C1 [Leesch, J. G.; Smilanick, J. L.; Tebbets, J. S.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Muhareb, J. S.; Hurley, J. M.; Jones, T. M.] DFA Calif, Fresno, CA 93721 USA. RP Smilanick, JL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM joe.smilanick@ars.usda.gov OI Jones, Thomas/0000-0001-5670-6529 FU Quimas, Ltd Santiago, Chile FX We thank Thomas Hanke of Quimas, Ltd Santiago, Chile for donation of materials, technical information about liners, and financial support of a portion of the research conducted at DFA of California. We thank Jane Tebbets for critical review of the manuscript. NR 19 TC 1 Z9 1 U1 1 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD SEP PY 2014 VL 63 BP 36 EP 40 DI 10.1016/j.cropro.2014.05.002 PG 5 WC Agronomy SC Agriculture GA AN1AE UT WOS:000340314300005 ER PT J AU Tupper, SK Werner, SJ Carlson, JC Pettit, SE Wise, JC Lindell, CA Linz, GM AF Tupper, Shelagh K. Werner, Scott J. Carlson, James C. Pettit, Susan E. Wise, John C. Lindell, Catherine A. Linz, George M. TI European starling feeding activity on repellent treated crops and pellets SO CROP PROTECTION LA English DT Article DE Anthraquinone; Sturnus vulgaris; SucraShield; Sucrose octanoate ester ID LABORATORY EFFICACY; STURNUS-VULGARIS; BIRDS; ANTHRAQUINONE; POPULATION; DAMAGE; ANTHRANILATE; BLACKBIRD; SUCROSE; RICE AB The varied diet of European starlings (Sturnus vulgaris L) can present challenges when working with starlings in experimental testing and holding situations and should be taken into account when testing repellents applied to food. Our purpose was to evaluate an anthraquinone-based repellent (Arkion Life Sciences, New Castle, DE, USA; active ingredient 50% 9,10-anthraquinone; hereafter anthraquinone) and SucraShield (TM), (Natural Forces, Davidson, NC, USA; active ingredient 40% sucrose octanoate esters) repellent for non-lethal protection of specialty crops (i.e., fruit, sweet corn) and grains. Our objectives were to evaluate (1) laboratory efficacy of anthraquinone applied topically to blueberries and sweet corn, (2) laboratory efficacy of anthraquinone applied to two pellet matrices, and (3) laboratory efficacy of SucraShield (TM) as a chemical repellent for European starlings. We found that anthraquinone was not an effective repellent for blueberries or sweet corn, although consumption of each matrix varied potentially due to sucrose content. Anthraquinone was an effective repellent on CU Bird Carrier pellets with 6275 ppm needed to achieve 80% repellency, whereas up to 35,000 ppm anthraquinone was not effective when the anthraquinone was not topically applied. SucraShield was not an effective repellent for starlings and in fact increased consumption of CU Bird Carrier as concentration increased. Published by Elsevier Ltd. C1 [Tupper, Shelagh K.; Werner, Scott J.; Carlson, James C.; Pettit, Susan E.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Wise, John C.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Lindell, Catherine A.] Michigan State Univ, Dept Zool, E Lansing, MI 48824 USA. [Linz, George M.] US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, Bismarck, ND 58501 USA. RP Tupper, SK (reprint author), US Anim & Plant Hlth Inspect Serv, USDA, Wildlife Serv, Natl Wildlife Res Ctr, 4101 LaPorte Ave, Ft Collins, CO 80521 USA. EM shelagh.k.tupper@aphis.usda.gov FU IR.4 Project; Michigan State University; USDA Specialty Crop Research Initiative FX We thank A. Mangan, and three anonymous reviewers for their thoughtful comments on previous versions of our manuscript. We also thank the analytical chemistry unit at the National Wildlife Research Center (Fort Collins, CO, U.S.A.) and Arkion Life Sciences (New Castle, DE, USA) for performing anthraquinone residue analyses. Arkion Life Sciences provided the anthraquinone-based repellent, and Natural Forces LLC (Davidson, NC, USA) provided the SucraShield (TM) product for our feeding experiments. Corporate collaborations do not imply endorsement by the United States Department of Agriculture. This project was supported in part by the IR.4 Project, Michigan State University, and the USDA Specialty Crop Research Initiative. NR 37 TC 5 Z9 5 U1 1 U2 13 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0261-2194 EI 1873-6904 J9 CROP PROT JI Crop Prot. PD SEP PY 2014 VL 63 BP 76 EP 82 DI 10.1016/j.cropro.2014.05.001 PG 7 WC Agronomy SC Agriculture GA AN1AE UT WOS:000340314300010 ER PT J AU Hewavitharana, SS Ruddell, D Mazzola, M AF Hewavitharana, Shashika Shivanthi Ruddell, David Mazzola, Mark TI Carbon source-dependent antifungal and nematicidal volatiles derived during anaerobic soil disinfestation SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Anaerobic soil disinfestation; Apple replant disease; Antifungal volatiles; Nematicidal volatiles ID APPLE REPLANT DISEASE; NAPUS SEED MEAL; ORGANIC-COMPOUNDS; RHIZOCTONIA-SOLANI; METABOLITES; AMENDMENTS; PATHOGENS; BACTERIA; EFFICACY; BSD AB Anaerobic soil disinfestation (ASD) has been shown to be effective in the control of a wide range of soil-borne plant pathogens but has not been examined as a means for disease control in perennial fruit crops such as apple. Since ASD has demonstrated a broad spectrum of biological activity, it may be well suited as an alternative to current fumigation-based control of apple replant disease (ARD) which is caused by a diverse pathogen complex. The efficacy of ASD for control of ARD pathogens was evaluated in growth chamber experiments using soils from two orchard sites having a history of the disease. Suppression of Pratylenchus penetrans apple root densities was dependent upon carbon source utilized during the ASD process. Volatiles emitted during the anaerobic phase from soils treated with ethanol, grass residues, or Brassica juncea seed meal as the carbon input effectively retarded growth of Rhizoctonia solani AG-5, Pythium ultimum and Fusarium oxysporum. Each carbon amendment generated a unique volatile profile produced in the treated orchard soil during ASD. Allyl isothiocyanate (AITC) and dimethyl trisulphide (DMTS) were emitted from B. juncea SM treated soils whereas the latter and 2-ethyl-1-hexanol were detected in soils treated with grass residues. When assayed individually using pure standards, Decanal, DMTS, and AITC retarded in vitro growth of all three fungal/oomycete pathogens. Nonanal was inhibitory toward only P. ultimum and R. solani AG-5, whereas 2-ethyl-1-hexanol only suppressed growth of P. ultimum. AITC and DMTS caused significantly higher mortality of P. penetrans compared to other tested volatiles. These findings demonstrate that carbon source-dependent volatile chemistries contribute significantly but not exclusively to suppression of certain ARD pathogens during the ASD process. C1 [Hewavitharana, Shashika Shivanthi] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Ruddell, David; Mazzola, Mark] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. RP Mazzola, M (reprint author), USDA ARS, Tree Fruit Res Lab, 1104 N Western Ave, Wenatchee, WA 98801 USA. EM mark.mazzola@ars.usda.gov FU USDA NIFA Methyl Bromide Alternatives Grants Program FX This work was funded in part through a grant to M. Mazzola from the USDA NIFA Methyl Bromide Alternatives Grants Program. NR 49 TC 10 Z9 12 U1 7 U2 48 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD SEP PY 2014 VL 140 IS 1 BP 39 EP 52 DI 10.1007/s10658-014-0442-5 PG 14 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AM8DF UT WOS:000340099900003 ER PT J AU Kim, YK Curry, EA Xiao, CL AF Kim, Y. K. Curry, E. A. Xiao, C. L. TI Infection of apple fruit by Sphaeropsis pyriputrescens in the orchard in relation to Sphaeropsis rot in storage SO EUROPEAN JOURNAL OF PLANT PATHOLOGY LA English DT Article DE Apple; Latent infection; Postharvest disease; Sphaeropsis pyriputrescens; Sphaeropsis rot ID BOTRYTIS-CINEREA; PEAR FRUIT; WASHINGTON-STATE; 1ST REPORT; DECAY; FLOWERS; DISEASE AB Sphaeropsis rot, caused by Sphaeropsis pyriputrescens, is an important postharvest disease of apple in the United States. The objectives of this study were to determine the timing of apple fruit infection in the orchard in relation to development of Sphaeropsis rot in storage and to identify infection courts and mode of penetration by S. pyriputrescens on apple fruit. Fruit of apple cvs Red Delicious, Golden Delicious, and Fuji were inoculated in the orchard from 3 weeks after petal fall to 2 weeks before harvest at 5 to 6-week intervals in three consecutive seasons. All fruit were harvested and stored at 0 A(0)C to monitor decay development. Light and scanning electron microscopy were used to examine the infection courts and mode of penetration of the fungus on/in the host tissues. At harvest, the fungus was re-isolated from the stem (pedicel), sepal, anther, or filament of the inoculated fruit, but decay did not develop on fruit. Sphaeropsis rot developed on inoculated fruit during cold storage beginning 1-3 months after harvest. Stem-end rot was prevalent on cv. Golden Delicious, whereas calyx-end rot was prevalent on cv. Fuji. Both stem- and calyx-end rots were common on cv. Red Delicious. Infection also occurred at lenticels on fruit skin, particularly on cv. Golden Delicious, but at low incidence. Relationships between the incidence of Sphaeropsis rot in stored apple fruit and the timing of inoculation in the orchard varied with cultivar and year. On cv. Red Delicious apples, the incidence of Sphaeropsis rot generally increased as the timing of infection approached harvest. Histological studies indicated that infection took place through natural openings of plant organs such as stomata on stems and sepals and lenticels on fruit skin. Fungal penetration also was observed at micro-cracks on the stem and sepal and at trichome sockets where mechanical damage occurred in sepals. Direct penetration was observed on the stem and sepal of fruit, but most invasions were restricted between the cuticle and the epidermis. Our results indicate that wounding is not required for infection of apple fruit by S. pyriputrescens, though it may facilitate infections. C1 [Kim, Y. K.] Washington State Univ, Dept Plant Pathol, Tree Fruit Res & Extens Ctr, Wenatchee, WA 98801 USA. [Curry, E. A.] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Xiao, C. L.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Xiao, CL (reprint author), USDA ARS, San Joaquin Valley Agr Sci Ctr, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM Chang-Lin.Xiao@ars.usda.gov FU Washington Tree Fruit Research Commission FX This research was supported by the Washington Tree Fruit Research Commission. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendations or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 20 TC 2 Z9 2 U1 2 U2 23 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0929-1873 EI 1573-8469 J9 EUR J PLANT PATHOL JI Eur. J. Plant Pathol. PD SEP PY 2014 VL 140 IS 1 BP 133 EP 143 DI 10.1007/s10658-014-0449-y PG 11 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AM8DF UT WOS:000340099900010 ER PT J AU Mendoza, FA Cichy, K Lu, RF Kelly, JD AF Mendoza, Fernando A. Cichy, Karen Lu, Renfu Kelly, James D. TI Evaluation of Canning Quality Traits in Black Beans (Phaseolus vulgaris L.) by Visible/Near-Infrared Spectroscopy SO FOOD AND BIOPROCESS TECHNOLOGY LA English DT Article DE Black beans; Canning quality; Color; Vis/NIR spectroscopy; Visual assessment ID SOLUBLE SOLIDS CONTENT; APPLE FRUIT FIRMNESS; IMAGE-ANALYSIS; FOOD QUALITY; DRY AB Black bean (Phaseolus vulgaris L.) processing presents unique challenges because of discoloration, breakage, development of undesirable textures, and off-flavors during canning and storage. These quality issues strongly affect processing standards and consumer acceptance for beans. In this research, visible and near-infrared (Vis/NIR) reflectance data for the spectral region of 400-2,500 nm were acquired from intact dry beans for predicting five canning quality traits, i.e., hydration coefficient (HC), visual appearance (APP) and color (COL), washed drained coefficient (WDC), and texture (TXT), using partial least squares regression (PLSR). A total of 471 bean samples harvested and canned in 2010, 2011, and 2012 were used for analysis. PLSR models based on the Vis/NIR data showed low predictive performance, as measured by correlation coefficient for prediction (R (pred)) for APP (R (pred) = 0.275-0.566) and TXT (R (pred) = 0.270-0.681), but better results for predicting HC (R (pred) = 0.517-0.810), WDC (R (pred) = 0.420-0.796), and COL (R (pred) < 0.533-0.758). In comparison, color measurements from a colorimeter on drained canned beans showed consistently good predictions for COL (R (pred) = 0.796-0.907). In spite of the low or relatively poor agreement among the sensory panelists as determined by multirater Kappa analysis (K (free) of 0.20 for APP and 0.18 for COL), a linear discriminant model using the Vis/NIR data was able to classify the canned bean samples into two sensory quality categories of "acceptable" and "unacceptable", based on panelists' ratings for APP and COL traits of canning beans, with classification accuracies of 72.6 % or higher. While Vis/NIR technique has the potential for assessing bean canning quality from intact dry beans, improvements in sensing and instrumentation are needed in order to meet the application requirements. C1 [Mendoza, Fernando A.; Cichy, Karen; Lu, Renfu] Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. [Kelly, James D.] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. RP Mendoza, FA (reprint author), Michigan State Univ, USDA ARS, 524S Shaw Lane,Room 105A-224, E Lansing, MI 48824 USA. EM fmendoza@msu.edu FU USDA Agricultural Research Service's in-house project [3635-21430-009-00D] FX This research was carried out as a part of the USDA Agricultural Research Service's in-house project 3635-21430-009-00D, Improved Quality in Dry Bean Using Genetic and Molecular Approaches. The authors thank Mr. Scott Shaw for his technical support in testing bean samples. NR 29 TC 7 Z9 7 U1 5 U2 25 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1935-5130 EI 1935-5149 J9 FOOD BIOPROCESS TECH JI Food Bioprocess Technol. PD SEP PY 2014 VL 7 IS 9 BP 2666 EP 2678 DI 10.1007/s11947-014-1285-y PG 13 WC Food Science & Technology SC Food Science & Technology GA AM7MC UT WOS:000340050400021 ER PT J AU Song, NF Aguilar, FX Butler, BJ AF Song, Nianfu Aguilar, Francisco X. Butler, Brett J. TI Cost-share program participation and family forest owners' past and intended future management practices SO FOREST POLICY AND ECONOMICS LA English DT Article DE Cost-share programs; Family forest owners; Propensity score matching; National Woodland Owner Survey ID ECONOMETRIC EVALUATION ESTIMATOR; PROPENSITY-SCORE; UNITED-STATES; TRAINING-PROGRAMS; PAYMENT PROGRAMS; PRIVATE FORESTS; SELECTION BIAS; CAUSAL; SOUTH; INCENTIVES AB Cost-share programs are commonly-used policy tools designed to influence management on privately-owned lands. Widely popular on agricultural lands, these programs and their association with landowner behavior have not been as thoroughly studied on forested lands. Based on a dataset of over 3500 observations and using propensity score matching to reduce possible selection bias, this study found that family forest owners in the U.S. Northern region enrolled in cost-share programs were more actively engaged in both silvicultural and conservation management activities than non-participants. These findings point to the capacity of cost-share public programs to promote better forest management. This study found that cost-share participation varied across size of forest holdings, owners' demographic characteristics, ownership objectives and forest location. Owners of smaller sized forestlands had a lower participation rate and might be a prime target group of future cost-share programs to widen forest and wildlife habitat management. (C) 2014 Elsevier B.V. All rights reserved. C1 [Song, Nianfu; Aguilar, Francisco X.] Univ Missouri, Dept Forestry, Columbia, MO 65211 USA. [Butler, Brett J.] US Forest Serv, USDA, Amherst, MA 01003 USA. RP Song, NF (reprint author), Univ Missouri, Dept Forestry, 203 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. FU Northern Research Station under U.S. Forest Service [09-PA-11242305-143] FX This research was partly funded by the Northern Research Station under U.S. Forest Service Agreement 09-PA-11242305-143. NR 69 TC 6 Z9 6 U1 3 U2 14 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1389-9341 EI 1872-7050 J9 FOREST POLICY ECON JI Forest Policy Econ. PD SEP PY 2014 VL 46 BP 39 EP 46 DI 10.1016/j.forpol.2014.06.003 PG 8 WC Forestry SC Forestry GA AN0XL UT WOS:000340307200005 ER PT J AU Wickham, J Wade, TG Riitters, KH AF Wickham, J. Wade, T. G. Riitters, K. H. TI An isoline separating relatively warm from relatively cool wintertime forest surface temperatures for the southeastern United States SO GLOBAL AND PLANETARY CHANGE LA English DT Article DE Afforestation; Albedo; Climate mitigation; MODIS; NLCD; Reforestation ID LAND-USE; SCALE DEFORESTATION; BOREAL FOREST; CLIMATE; SNOWMELT; CANOPY; IMPACT; EVAPOTRANSPIRATION; ALGORITHM; RADIATION AB Forest-oriented climate mitigation policies promote forestation as a means to increase uptake of atmospheric carbon to counteract global warming. Some have pointed out that a carbon-centric forest policy may be overstated because it discounts biophysical aspects of the influence of forests on climate. In extra-tropical regions, many climate models have shown that forests tend to be warmer than grasslands and croplands because forest albedos tend to be lower than non-forest albedos. A lower forest albedo results in higher absorption of solar radiation and increased sensible warming that is not offset by the cooling effects of carbon uptake in extra-tropical regions. However, comparison of forest warming potential in the context of climate models is based on a coarse classification system of tropical, temperate, and boreal. There is considerable variation in climate within the broad latitudinal zonation of tropical, temperate, and boreal, and the relationship between biophysical (albedo) and biogeochemical (carbon uptake) mechanisms may not be constant within these broad zones. We compared wintertime forest and non-forest surface temperatures for the southeastern United States and found that forest surface temperatures shifted from being warmer than non-forest surface temperatures north of approximately 36 degrees N to cooler south of 36 degrees N. Our results suggest that the biophysical aspects of forests' influence on climate reinforce the biogeochemical aspects of forests' influence on climate south of 36 degrees N. South of 36 degrees N, both biophysical and biogeochemical properties of forests appear to support forestation as a climate mitigation policy. We also provide some quantitative evidence that evergreen forests tend to have cooler wintertime surface temperatures than deciduous forests that may be attributable to greater evapotranspiration rates. Published by Elsevier B.V. C1 [Wickham, J.; Wade, T. G.] US EPA, Off Res & Dev, Div Environm Sci, Res Triangle Pk, NC 27711 USA. [Riitters, K. H.] US Forest Serv, USDA, Southern Res Stn, Res Triangle Pk, NC 27709 USA. RP Wickham, J (reprint author), US EPA, NERL, MD E243-05, Res Triangle Pk, NC 27701 USA. EM wickham.james@epa.gov FU United States Environmental Protection Agency (US EPA), through its Office of Research and Development FX The United States Environmental Protection Agency (US EPA), through its Office of Research and Development, funded and managed the research described here. It has been subjected to Agency review and approved for publication. Approval does not signify that the contents reflect the views of US EPA. We thank Megan Mehaffey (US EPA) and X anonymous reviewers for their helpful comments on earlier drafts of the paper. We also thank Y. Shuai and C. Schaaf for providing the Landsat-based albedo data and for their insights on various aspects of albedo. The mention of trade names should not be interpreted as an endorsement or recommendation of use. NR 38 TC 4 Z9 4 U1 1 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0921-8181 EI 1872-6364 J9 GLOBAL PLANET CHANGE JI Glob. Planet. Change PD SEP PY 2014 VL 120 BP 46 EP 53 DI 10.1016/j.gloplacha.2014.05.012 PG 8 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA AM9SS UT WOS:000340221900005 ER PT J AU Selmants, PC Litton, CM Giardina, CP Asner, GP AF Selmants, Paul C. Litton, Creighton M. Giardina, Christian P. Asner, Gregory P. TI Ecosystem carbon storage does not vary with mean annual temperature in Hawaiian tropical montane wet forests SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon balance; carbon stocks; climate change; elevation gradient; Hawaii; tropical wet forest ID SOIL ORGANIC-MATTER; CLIMATE-CHANGE; RAIN-FORESTS; ELEVATIONAL TRANSECT; DECOMPOSITION RATES; ABOVEGROUND BIOMASS; PERUVIAN ANDES; GLOBAL CHANGE; PRODUCTIVITY; RESPONSES AB Theory and experiment agree that climate warming will increase carbon fluxes between terrestrial ecosystems and the atmosphere. The effect of this increased exchange on terrestrial carbon storage is less predictable, with important implications for potential feedbacks to the climate system. We quantified how increased mean annual temperature (MAT) affects ecosystem carbon storage in above-and belowground live biomass and detritus across a well-constrained 5.2 degrees C MAT gradient in tropical montane wet forests on the Island of Hawaii. This gradient does not systematically vary in biotic or abiotic factors other than MAT (i.e. dominant vegetation, substrate type and age, soil water balance, and disturbance history), allowing us to isolate the impact of MAT on ecosystem carbon storage. Live biomass carbon did not vary predictably as a function of MAT, while detrital carbon declined by similar to 14 Mg of carbon ha(-1) for each 1 degrees C rise in temperature - a trend driven entirely by coarse woody debris and litter. The largest detrital pool, soil organic carbon, was the most stable with MAT and averaged 48% of total ecosystem carbon across the MAT gradient. Total ecosystem carbon did not vary significantly with MAT, and the distribution of ecosystem carbon between live biomass and detritus remained relatively constant across the MAT gradient at similar to 44% and similar to 56%, respectively. These findings suggest that in the absence of alterations to precipitation or disturbance regimes, the size and distribution of carbon pools in tropical montane wet forests will be less sensitive to rising MAT than predicted by ecosystem models. This article also provides needed detail on how individual carbon pools and ecosystem-level carbon storage will respond to future warming. C1 [Selmants, Paul C.; Litton, Creighton M.] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, Hilo, HI 96720 USA. [Asner, Gregory P.] Carnegie Inst Sci, Dept Global Ecol, Stanford, CA 94305 USA. RP Selmants, PC (reprint author), Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. EM selmants@hawaii.edu RI Giardina, Christian/C-3120-2011; Asner, Gregory/G-9268-2013; OI Giardina, Christian/0000-0002-3431-5073; Asner, Gregory/0000-0001-7893-6421; Selmants, Paul/0000-0001-6211-3957 FU National Science Foundation [DEB-0816486]; USDA Forest Service, Institute of Pacific Islands Forestry, Pacific Southwest Research Station [09-JV-11272177-029, 12-JV-11272139-047]; College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa via the USDA National Institute of Food and Agriculture, Hatch and McIntire-Stennis Programs [HAW00132-H, HAW01127-H, HAW00188-M, HAW01123-M] FX We thank the USDA Forest Service and State of Hawaii Department of Land and Natural Resources Division of Forestry and Wildlife for access to the Hawaii Experimental Tropical Forest, and the US Fish and Wildlife Service for access to the Hakalau Forest National Wildlife Refuge. J. Albano, M. Long, J. Johansen, S. Laursen, D. Iwashita, C. Perry, B. Hwang, and J. Schulten assisted with data collection. This study was funded by the National Science Foundation (Ecosystem Science Program; DEB-0816486); the USDA Forest Service, Institute of Pacific Islands Forestry, Pacific Southwest Research Station (Research Joint Ventures 09-JV-11272177-029 and 12-JV-11272139-047); and the College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa via the USDA National Institute of Food and Agriculture, Hatch and McIntire-Stennis Programs (HAW00132-H, HAW01127-H, HAW00188-M, and HAW01123-M). The Carnegie Airborne Observatory is made possible by the Avatar Alliance Foundation, Grantham Foundation for the Protection of the Environment, Gordon and Betty Moore Foundation, the John D. and Catherine T. MacArthur Foundation, W. M. Keck Foundation, the Margaret A. Cargill Foundation, Mary Anne Nyburg Baker and G. Leonard Baker Jr., and William R. Hearst III. The authors declare no conflict of interest. NR 61 TC 8 Z9 8 U1 9 U2 77 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD SEP PY 2014 VL 20 IS 9 BP 2927 EP 2937 DI 10.1111/gcb.12636 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AN0PU UT WOS:000340287300020 PM 24838341 ER PT J AU Kang, BG McMahan, C AF Kang, Byung-Guk McMahan, Colleen TI Medium and calcium nitrate influence on shoot regeneration from leaf explants of guayule SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Parthenium argentatum Gray; cis-1, 4-Polyisoprene; Calcium nitrate tetrahydrate; Basal media; Regeneration ID IN-VITRO-PROPAGATION; PARTHENIUM-ARGENTATUM; GROWTH; RUBBER; PLANTS; INVITRO; TRANSFORMATION; TEMPERATURE; WALNUT; SHRUB AB Basal media including Murashige and Skoog (MS), Woody Plant Medium (WPM), and Driver and Kuniyuki (DKW) media were compared for use in guayule regeneration and propagation. Explants grown on MS basal medium showed large size calli with multiple shoots, but the calli produced on DKW medium were of higher quality, and longer true shoots were produced. Adventitious root formation was best in WPM basal medium. A comparison of macro and micro nutrient levels in MS, DKW, and WPM media revealed significant differences in the calcium levels. Under controlled conditions, guayule plants in vitro incorporated calcium into plant tissues in proportion to the amount available. Moreover, the addition of calcium nitrate tetrahydrate at moderate levels into MS medium significantly improved the emergence of single, elongated true shoots, suggesting a requirement for calcium in guayule tissue culture. We have further explored selection agent (antibiotic) concentration for the identification of transgenic plants using a spectinomycin resistance gene (aadA). Based on the results, we have improved tissue culture protocols for guayule including spectinomycin as the selection agent. Published by Elsevier B.V. C1 [Kang, Byung-Guk; McMahan, Colleen] USDA ARS, Western Reg Res Lab, Albany, CA 94710 USA. RP McMahan, C (reprint author), USDA ARS, Western Reg Res Lab, Albany, CA 94710 USA. EM colleen.mcmahan@ars.usda.gov NR 34 TC 1 Z9 2 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2014 VL 60 BP 92 EP 98 DI 10.1016/j.indcrop.2014.06.005 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA AN1BT UT WOS:000340318400014 ER PT J AU Biswas, A Kim, S Selling, GW Cheng, HN AF Biswas, Atanu Kim, S. Selling, Gordon W. Cheng, H. N. TI Conversion of agricultural residues to carboxymethylcellulose and carboxymethylcellulose acetate SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Agricultural by-products; Carboxymethylcellulose; CMC acetate; Wheat straw; Barley straw; Rice hull ID WHEAT-STRAW; BY-PRODUCTS; ENZYMATIC SACCHARIFICATION; CELLULOSE-ACETATE; FERMENTATION; ETHANOL; COTTON; PRETREATMENT; IODINE; ACID AB In view of continuing interest in the utilization of agricultural by-products, we have converted cellulose, wheat straw, barley straw, and rice hull into carboxymethylcellulose (CMC), using both conventional heat and microwave-assisted synthesis. The application of microwave technology has enabled an improved synthesis of CMC to be conducted, with similar degrees of substitution as conventional heat (3 h) but reduced reactions times (30 min). The CMC obtained can be acetylated to form carboxymethylcellulose acetate (CMC acetate). The FT-IR and solid state C-13 NMR spectra of these materials have been characterized. With variations in the degrees of substitution of carboxymethyl and acetyl groups, a family of CMC acetate products can be made. The polymer may perhaps find applications as filtration membranes, functional fillers in polymers, and additives in commercial formulations. Published by Elsevier B.V. C1 [Biswas, Atanu; Kim, S.; Selling, Gordon W.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Cheng, H. N.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Biswas, A (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM atanu.biswas@ars.usda.gov; sanghoon.kim@ars.usda.gov; gordon.selling@ars.usda.gov; hn.cheng@ars.usda.gov NR 36 TC 6 Z9 6 U1 0 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2014 VL 60 BP 259 EP 265 DI 10.1016/j.indcrop.2014.06.004 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA AN1BT UT WOS:000340318400035 ER PT J AU Chen, MH Dien, BS Vincent, ML Below, FE Singh, V AF Chen, Ming-Hsu Dien, Bruce S. Vincent, Michael L. Below, Frederick E. Singh, Vijay TI Effect of harvest maturity on carbohydrates for ethanol production from sugar enhanced temperate x tropical maize hybrid SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Temperate x tropical maize (TTM); Tropical maize; Biofuel; Fermentation; Ethanol ID LIGNEOUS PLANTS; CORN STOVER; SORGHUM AB A northern adapted sugar maize (Zea mays L.) hybrid was bred by crossing temperate x tropical maize for bioethanol production. Temperate x tropical maize (TTM) has a prolonged vegetative growth and accumulates more sugar in the stalk compared to its respective tropical and temperature parents (White et al., 2012). In this study, the sugar concentration in the stalk was further increased by preventing pollination by shoot bagging (covering) ears. It was observed that starch content was eliminated and sugar content increased to 30.1% (w/w) in the stalks. The whole plant biomass (grain, sugars and stover) was evaluated for ethanol production. Ethanol produced from sugars and starch was comparable from milk (R3) to dent (R5) reproductive growth stages between pollinated and non-pollinated TTM, indicating that soluble sugar contents increased correspondingly with decreased starch contents. Temperate x tropical maize samples from both pollinated and non-pollinated treatments had high extractives. Glucan and xylan percentages were increased in non-pollinated extractive free samples. Ethanol produced from cellulosic material was similar for both treatments. The total ethanol yield (from starch, sugar and stover) was comparable from milk (R3) to dent (R5) stages and ranged from 0.20 to 0.22 g/g biomass. The pollinated TTM produced higher biomass in the field and resulted in 624.7 gal of ethanol per acre of land. Future research in TTM could be focused on increasing biomass yield of non-pollinated TTM. (C) 2014 Elsevier B.V. All rights reserved. C1 [Chen, Ming-Hsu; Singh, Vijay] Univ Illinois, Agr & Biol Engn, Urbana, IL 61801 USA. [Dien, Bruce S.] USDA ARS, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. [Vincent, Michael L.; Below, Frederick E.] Univ Illinois, Urbana, IL 61801 USA. RP Singh, V (reprint author), Univ Illinois, Agr & Biol Engn, 1304W,Penn Ave, Urbana, IL 61801 USA. EM vsingh@illinois.edu FU multi-state Agricultural Experiment Station project [NC-1200] FX We would like to thank Ms. Wei Liu from Agricultural and Biological Engineering department, University of Illinois at Urbana-Champaign for her help on HPLC and NREL standard procedures; Ms. Juliann R. Seebauer, Crop Sciences department, University of Illinois at Urbana-Champaign for her help in editing this manuscript. This research was made possible with partial funding from the multi-state Agricultural Experiment Station project NC-1200. NR 20 TC 2 Z9 2 U1 2 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2014 VL 60 BP 266 EP 272 DI 10.1016/j.indcrop.2014.06.015 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA AN1BT UT WOS:000340318400036 ER PT J AU Liu, JG Bronson, KF Thorp, KR Mon, J Badaruddin, M McCloskey, WB Ray, DT Chu, QQ Wang, GY AF Liu, Jiangang Bronson, Kevin F. Thorp, Kelly R. Mon, Jarai Badaruddin, Mohammad McCloskey, William B. Ray, Dennis T. Chu, Qingquan Wang, Guangyao TI Lesquerella seed and oil yield response to split-applied N fertilizer SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Lesquerella; Nitrogen; Seed yield; Oil yield ID NITROGEN-USE EFFICIENCY; FENDLERI; GROWTH; REGISTRATION; TRAITS; DATE AB Agronomic management information is critical for successful commercial production of new crops such as lesquerella [Physaria fendleri (A. Gray) O'Kane & Al-Shehbaz]. Response of lesquerella to six nitrogen (N) fertilizer rates under well-watered and water-stressed treatments were studied in irrigated desert conditions in the southwestern United States to establish guidelines for lesquerella N management. Seed and oil yield, seed and straw N concentrations, N harvest index, and total N uptake were recorded at harvest and destructive biomass samples were also collected during growing season for plant N status. Although soil NO3-N was low due to a cover crop to remove excessive N in the soil, pre-plant N fertilizer did not affect lesquerella growth at the early growth stage. For the treatments with high N fertilizer rates (224 and 336 kg ha(-1)), lesquerella plant N concentrations were relatively stable before the first bloom, decreased drastically until mid-bloom (one month after the first bloom), and then continued to decrease at a lower rate until harvest. As N fertilizer rates increased, seed and straw N concentration increased linearly under both well-watered and water-stressed conditions. Lesquerella seed and oil yield had a quadratic relationship with N fertilizer rate. Because seed oil concentration decreased as N fertilizer increased, N fertilizer rate for the optimum oil yield was lower than the N fertilizer rate for the optimum seed yield. According to seed and oil yield as well as plant N status under different N fertilizer rates, we provided a guideline for N fertilizer management for lesquerella. (C) 2014 Elsevier B.V. All rights reserved. C1 [Liu, Jiangang; Chu, Qingquan] China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China. [Bronson, Kevin F.; Thorp, Kelly R.; Mon, Jarai] USDA ARS, US AridLand Agr Res Ctr, Maricopa, AZ 85138 USA. [Badaruddin, Mohammad] Univ Arizona, Maricopa Agr Ctr, Maricopa, AZ 85138 USA. [McCloskey, William B.; Ray, Dennis T.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Liu, Jiangang; Wang, Guangyao] Univ Calif, Desert Res & Extens Ctr, Div Agr & Nat Resources, Holtville, CA 92250 USA. RP Wang, GY (reprint author), Univ Calif, Desert Res & Extens Ctr, Div Agr & Nat Resources, 1004 E Holton Rd, Holtville, CA 92250 USA. EM samwang@ucanr.edu RI Thorp, Kelly/C-2013-2009 OI Thorp, Kelly/0000-0001-9168-875X FU Sun Grant Initiative [T0013G-I] FX We wish to thank the Sun Grant Initiative Grant No. T0013G-I for financial support of this project and Suzette Maneely for technical assistance on the project. NR 26 TC 1 Z9 1 U1 2 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2014 VL 60 BP 273 EP 279 DI 10.1016/j.indcrop.2014.06.024 PG 7 WC Agricultural Engineering; Agronomy SC Agriculture GA AN1BT UT WOS:000340318400037 ER PT J AU Alves, AAC Manthey, L Isbell, T Ellis, D Jenderek, MM AF Cunha Alves, Alfredo Augusto Manthey, Linda Isbell, Terry Ellis, David Jenderek, Maria M. TI Diversity in oil content and fatty acid profile in seeds of wild cassava germplasm SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Genetic resources; Germplasm diversity; Manihot; NMR; Gas chromatography ID MANIHOT-ESCULENTA; BIODIESEL PRODUCTION; BIOLOGY; ORIGIN AB Cassava (Manihot esculenta) is the only commercial species of the Manihot genus, cultivated for its starchy tuber roots. However, cassava seeds are known to be rich in oils and fats, there are scant reports on the content and properties of oil from cassava seeds and its wild relatives. Wild Manihot species usually produce a higher number of seeds with a large diversity in shape and weight. Seeds of 106 accessions belonging to 12 species of Manihot from the collection of Embrapa Cassava and Fruits were evaluated for oil content by NMR and fatty acids composition by gas chromatography. The oil content ranged from 17.2% (M. caerulescens) to 30.7% (M. flabellifolia) and the species clustered into eight different groups based on the oil content. Five fatty acids were found in all species with the average content of the fatty acids being: linoleic (C18:2) 61.5%; oleic (C18:1) 20.0%; palmitic (C16:0) 12.3%; stearic (C18:0) 4.5%; and linolenic (C18:3) 1.7%. The content of fatty acids varied significantly between species as well as between accessions within a species. The highest content of linoleic acid was in seeds of M. peruviana, M. pseudoglaziovii, M. cecropiaefolia, M. flabellifolia, M. glaziovii and M. carthaginensis (average of 65%); and the highest level of oleic acid was in M. caerulescens, M. esculenta, M. anomala, M. dichotoma and M. tomentosa (average of 23%). The collection of Embrapa's Manihot germplasm is a valuable source for cassava breeding programs, containing a large variability in seed size, oil content and fatty acid composition. The oil from seeds of wild Manihot species may be equally valuable for industrial uses as oil from seeds of other Euphorbiaceae species. Published by Elsevier B.V. C1 [Cunha Alves, Alfredo Augusto] EMBRAPA, Secretaria Relagoes Int, BR-70770900 Brasilia, DF, Brazil. [Manthey, Linda; Isbell, Terry] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Ellis, David] Int Potato Ctr, Lima 12, Peru. [Cunha Alves, Alfredo Augusto; Jenderek, Maria M.] USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80522 USA. RP Jenderek, MM (reprint author), USDA ARS, Natl Ctr Genet Resources Preservat, Ft Collins, CO 80522 USA. EM maria.jenderek@ars.usda.gov NR 31 TC 5 Z9 5 U1 0 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2014 VL 60 BP 310 EP 315 DI 10.1016/j.indcrop.2014.06.025 PG 6 WC Agricultural Engineering; Agronomy SC Agriculture GA AN1BT UT WOS:000340318400042 ER PT J AU Moser, BR Zheljazkov, VD Bakota, EL Evangelista, RL Gawde, A Cantrell, CL Winkler-Moser, JK Hristov, AN Astatkie, T Jeliazkova, E AF Moser, Bryan R. Zheljazkov, Valtcho D. Bakota, Erica L. Evangelista, Roque L. Gawde, Archana Cantrell, Charles L. Winkler-Moser, Jill K. Hristov, Alexander N. Astatkie, Tess Jeliazkova, Ekaterina TI Method for obtaining three products with different properties from fennel (Foeniculum vulgare) seed SO INDUSTRIAL CROPS AND PRODUCTS LA English DT Article DE Distillation; Essential oil; Foeniculum vulgare; Lipid; Steam distillation ID ESSENTIAL OIL YIELD; JUNIPERUS-SCOPULORUM TREES; CARBON-DIOXIDE EXTRACTION; DISTILLATION TIME; ANTIOXIDANT ACTIVITY; PETROSELINIC ACID; AROMATIC PLANTS; HYDRODISTILLATION; CAPACITY; HERBS AB The objectives of this study were to determine the effects of distillation time (DT; 15-1080 min) on yield, composition, and antioxidant capacity of fennel (Foeniculum vulgare) seed essential oil (EO) as well as on the yield, composition, and properties of lipids extracted from steam-distilled fennel seeds (15-600 min). EO yield increased with increasing DT to a maximum of 1.375% at 1080 min. The principal constituent was estragole, comprising 82-91% of the overall content. Other species included limonene, fenchone, and anethole. Antioxidant capacity of the EO was essentially unaffected by DT, with capacities ranging from 11.2 to 20.6 mu mol Trolox/g. The yield of lipids extracted from steam distilled fennel seeds was unaffected by DT and ranged from 21.7 to 22.8 mass%. The fatty acid composition was also unaffected by DT, and the major constituents were petroselenic (67.0-71.3%) and oleic (12.0-16.4%) acids. The concentrations of tocopherols, tocotrienols, and phytosterols were unaffected by DT whereas unsaponifiables and EO content in lipids decreased with increasing DT. Acid value, kinematic viscosity, peroxide value, and pour point increased with increasing DT, whereas density decreased. Induction period, heteroatom content, and Gardner color were unaffected by DT. As DT increased, in vitro degradability of defatted, steam-distilled fennel seeds decreased. In summary, longer DT negatively impacted feed quality of steam-distilled, defatted seed meal and lipid quality but did not significantly affect EO composition and antioxidant capacity. Published by Elsevier B.V. C1 [Moser, Bryan R.; Bakota, Erica L.; Evangelista, Roque L.; Winkler-Moser, Jill K.] USDA ARS, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. [Zheljazkov, Valtcho D.; Jeliazkova, Ekaterina] Univ Wyoming, Sheridan Res & Extens Ctr, Sheridan, WY 82801 USA. [Gawde, Archana; Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res, University, MS 38677 USA. [Hristov, Alexander N.] Penn State Univ, Dept Anim Sci, University Pk, PA 16802 USA. [Astatkie, Tess] Dalhousie Univ, Fac Agr, Truro, NS B2N 5E3, Canada. RP Moser, BR (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Bryan.Moser@ars.usda.gov RI Astatkie, Tess/J-7231-2012; OI Astatkie, Tess/0000-0002-9779-8789; Gawde, Archana/0000-0002-6495-3850 NR 38 TC 1 Z9 1 U1 2 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-6690 EI 1872-633X J9 IND CROP PROD JI Ind. Crop. Prod. PD SEP PY 2014 VL 60 BP 335 EP 342 DI 10.1016/j.indcrop.2014.06.017 PG 8 WC Agricultural Engineering; Agronomy SC Agriculture GA AN1BT UT WOS:000340318400045 ER PT J AU Song, X Clark, KS Helliker, BR AF Song, Xin Clark, Kenneth S. Helliker, Brent R. TI Interpreting species-specific variation in tree-ring oxygen isotope ratios among three temperate forest trees SO PLANT CELL AND ENVIRONMENT LA English DT Article DE among-species variation; p(ex); stable oxygen isotope; tree-ring cellulose ID NET ECOSYSTEM EXCHANGE; LEAF WATER; STABLE-ISOTOPES; INTERSPECIFIC VARIATION; SUMMER PRECIPITATION; INVASIVE INSECTS; RICINUS-COMMUNIS; QUERCUS-PETRAEA; VAPOR-PRESSURE; SOIL-WATER AB Although considerable variation has been documented in tree-ring cellulose oxygen isotope ratios (O-18(cell)) among co-occurring species, the underlying causes are unknown. Here, we used a combination of field measurements and modelling to investigate the mechanisms behind variations in late-wood O-18(cell) (O-18(lc)) among three co-occurring species (chestnut oak, black oak and pitch pine) in a temperate forest. For two growing seasons, we quantified among-species variation in O-18(lc), as well as several variables that could potentially cause the O-18(lc) variation. Data analysis based on the O-18(cell) model rules out leaf water enrichment (O-18(lw)) and tree-ring formation period (t), but highlights source water O-18 (O-18(sw)) as an important driver for the measured difference in O-18(lc) between black and chestnut oak. However, the enriched O-18(lc) in pitch pine relative to the oaks could not be sufficiently explained by consideration of the above three variables only, but rather, we show that differences in the proportion of oxygen exchange during cellulose synthesis (p(ex)) is most likely a key mechanism. Our demonstration of the relevance of some species-specific features (or lack thereof) to O-18(cell) has important implications for isotope based ecophysiological/paleoclimate studies. C1 [Song, Xin; Helliker, Brent R.] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA. [Clark, Kenneth S.] US Forest Serv, Silas Little Expt Forest, USDA, New Lisbon, NJ 08064 USA. RP Song, X (reprint author), Univ Penn, Dept Biol, Philadelphia, PA 19104 USA. EM xinsong@sas.upenn.edu FU National Science Foundation [IOS-0950998] FX We would like to thank Erin Wiley and Dustin Bronson for help in the field, David Vann for isotope analysis and Nicholas Distefano for water extraction. We thank Margaret Barbour, Graham Farquhar, Ansgar Kahmen and two anonymous reviewers for their constructive comments on an early version of the manuscript. This work was supported by the National Science Foundation under award number IOS-0950998 (to B. R. H. and X.S.). NR 71 TC 14 Z9 14 U1 5 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0140-7791 EI 1365-3040 J9 PLANT CELL ENVIRON JI Plant Cell Environ. PD SEP PY 2014 VL 37 IS 9 BP 2169 EP 2182 DI 10.1111/pce.12317 PG 14 WC Plant Sciences SC Plant Sciences GA AN0RT UT WOS:000340292400018 PM 24588709 ER PT J AU Rodgers, J Fortier, C Kang, SY Montalvo, J Von Hoven, T AF Rodgers, James Fortier, Chanel Kang, Sho Yeung Montalvo, Joseph Von Hoven, Terri TI Cotton Fiber Moisture Measurements: A Comparative Evaluation SO AATCC JOURNAL OF RESEARCH LA English DT Article DE Cotton; Fiber Moisture; Karl Fischer Titration; Moisture; NIR; Oven Drying AB A commonly-used standard method for measuring cotton fiber moisture is the oven-drying method (moisture content equal weight loss). However, several commercial instruments are available for measuring fiber moisture content. A comparative evaluation program was implemented to determine the capabilities of laboratory moisture instrumentation/methods and to develop a standard format to quickly compare different units (logistical and technical attributes). Fifteen instruments were evaluated and compared to an oven-drying (reference) method. The best measurement agreements to the reference method were obtained for most small oven analyzers and near-infrared (NIR) analyzers, with few outliers and low residuals. A comprehensive comparative matrix was developed to assist the user decision-making process for selecting the best overall instrument to meet their fiber moisture measurement needs. C1 [Rodgers, James; Fortier, Chanel; Kang, Sho Yeung; Montalvo, Joseph; Von Hoven, Terri] USDA ARS, SRRC, New Orleans, LA 70124 USA. RP Rodgers, J (reprint author), USDA ARS, SRRC, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM james.rodgers@ars.usda.gov FU Cotton Incorporated (Cary, NC) [58-6435-1-568] FX The authors wish to acknowledge the outstanding work by Jeannine Moraitis and Sarah Lillis. This research was partially funded by Cotton Incorporated (Cary, NC) under project number 58-6435-1-568. NR 22 TC 0 Z9 0 U1 1 U2 1 PU AMER ASSOC TEXTILE CHEMISTS COLORISTS PI RES TRIANGLE PK PA PO BOX 12215, RES TRIANGLE PK, NC 27709 USA SN 2330-5517 J9 AATCC J RES JI AATCC J. Res. PD SEP-OCT PY 2014 VL 1 IS 5 BP 24 EP 33 PG 10 WC Materials Science, Textiles SC Materials Science GA CU6AY UT WOS:000363615100004 ER PT J AU Chiu, CJ Chang, ML Zhang, FF Li, T Gensler, G Schleicher, M Taylor, A AF Chiu, C. J. Chang, M. L. Zhang, F. F. Li, T. Gensler, G. Schleicher, M. Taylor, A. TI Major American dietary patterns are related to age-related macular degeneration SO ACTA OPHTHALMOLOGICA LA English DT Meeting Abstract C1 [Chiu, C. J.; Chang, M. L.; Schleicher, M.; Taylor, A.] Tufts Univ, USDA, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Chiu, C. J.] Tufts Univ, Sch Med, Dept Ophthalmol, Boston, MA 02111 USA. [Zhang, F. F.] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Li, T.] Harvard Univ, Brigham & Womens Hosp, Sch Med, Channing Div Network Med, Boston, MA 02115 USA. [Gensler, G.] EMMES Corp, AREDS Coordinating Ctr, Rockville, MD USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-375X EI 1755-3768 J9 ACTA OPHTHALMOL JI Acta Ophthalmol. PD SEP PY 2014 VL 92 SU 253 SI SI MA F061 DI 10.1111/j.1755-3768.2014.F061.x PG 3 WC Ophthalmology SC Ophthalmology GA V43SZ UT WOS:000209702400694 ER PT J AU Macho, AP Schwessinger, B Ntoukakis, V Brutus, A Segonzac, C Roy, S Kadota, Y Oh, MH Sklenar, J Derbyshire, P Lozano-Duran, R Malinovsky, FG Monaghan, J Menke, FL Huber, SC He, SY Zipfel, C AF Macho, A. P. Schwessinger, B. Ntoukakis, V. Brutus, A. Segonzac, C. Roy, S. Kadota, Y. Oh, M. -H. Sklenar, J. Derbyshire, P. Lozano-Duran, R. Malinovsky, F. Gro Monaghan, J. Menke, F. L. Huber, S. C. He, S. Yang Zipfel, C. TI A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation SO FEBS JOURNAL LA English DT Meeting Abstract CT FEBS EMBO 2014 Conference CY AUG 30-SEP 04, 2014 CL Paris, FRANCE SP FEBS, EMBO C1 [Macho, A. P.; Schwessinger, B.; Ntoukakis, V.; Segonzac, C.; Roy, S.; Kadota, Y.; Lozano-Duran, R.; Malinovsky, F. Gro; Monaghan, J.; Menke, F. L.; Zipfel, C.] Sainsbury Lab, Norwich, Norfolk, England. [Brutus, A.; He, S. Yang] Michigan State Univ, DOE Plant Res Lab, E Lansing, MI 48824 USA. [He, S. Yang] Michigan State Univ, Howard Hughes Med Inst, E Lansing, MI 48824 USA. [Oh, M. -H.; Huber, S. C.] Univ Illinois, USDA ARS, Urbana, IL 61801 USA. [Oh, M. -H.; Huber, S. C.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Oh, M. -H.] Chungnam Natl Univ, Dept Biol Sci, Taejon 305764, South Korea. RI Kadota, Yasuhiro/A-9406-2011 OI Kadota, Yasuhiro/0000-0002-4782-1418 NR 0 TC 0 Z9 0 U1 1 U2 9 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1742-464X EI 1742-4658 J9 FEBS J JI FEBS J. PD SEP PY 2014 VL 281 SU 1 SI SI MA WED-324 BP 726 EP 726 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CP1WE UT WOS:000359666804297 ER PT J AU Slavokhotova, AA Naumann, TA Price, N Rogozhin, EA Andreev, YA Vassilevski, AA Odintsova, TI AF Slavokhotova, A. A. Naumann, T. A. Price, N. Rogozhin, E. A. Andreev, Y. A. Vassilevski, A. A. Odintsova, T. I. TI Novel mode of action in plant defense peptides SO FEBS JOURNAL LA English DT Meeting Abstract CT FEBS EMBO 2014 Conference CY AUG 30-SEP 04, 2014 CL Paris, FRANCE SP FEBS, EMBO DE hevein-like antimicrobial peptide; fungalysin; Zn-metalloproteinase; chitinase; Triticum kiharae; Fusarium verticillioides C1 [Slavokhotova, A. A.] Vavilov Inst Gen Genet, Plant Genet, Moscow, Russia. [Naumann, T. A.] USDA ARS NCAUR, Foodborne Pathogens & Mycol Res Unit, Peoria, IL USA. [Price, N.] USDA ARS NCAUR, Renewable Prod Technol Res Unit, Peoria, IL USA. [Rogozhin, E. A.; Andreev, Y. A.; Vassilevski, A. A.] Russian Acad Sci, Shemyakin & Ovchinnikov Inst Bioorgan Chem, Moscow, Russia. [Odintsova, T. I.] Vavilov Inst Gen Genet, Moscow, Russia. RI Andreev, Yaroslav/C-6627-2012 NR 0 TC 0 Z9 0 U1 0 U2 1 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1742-464X EI 1742-4658 J9 FEBS J JI FEBS J. PD SEP PY 2014 VL 281 SU 1 SI SI MA WED-401 BP 753 EP 753 PG 1 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CP1WE UT WOS:000359666804371 ER PT J AU Bramwell, L AF Bramwell, Lincoln TI Ahead of the Flaming Front A Life on Fire SO PACIFIC NORTHWEST QUARTERLY LA English DT Book Review C1 [Bramwell, Lincoln] US Forest Serv, Washington, DC 20250 USA. RP Bramwell, L (reprint author), US Forest Serv, Washington, DC 20250 USA. NR 1 TC 0 Z9 0 U1 0 U2 0 PU UNIV WASHINGTON PI SEATTLE PA 4045 BROOKLYN AVENUE NE, SEATTLE, WA 98105 USA SN 0030-8803 J9 PAC NORTHWEST QUART JI Pac. Northwest Q. PD FAL PY 2014 VL 105 IS 4 BP 201 EP 202 PG 2 WC History SC History GA CY1EP UT WOS:000366149800015 ER PT J AU Wiggins, DA Schnell, GD Augustine, DJ AF Wiggins, David A. Schnell, Gary D. Augustine, David J. TI DISTRIBUTION AND NESTING SUCCESS OF FERRUGINOUS HAWKS AND SWAINSON'S HAWKS ON AN AGRICULTURAL LANDSCAPE IN THE GREAT PLAINS SO SOUTHWESTERN NATURALIST LA English DT Article AB We studied land-cover associations at nest sites and reproductive success of two Buteo species of conservation concern on the southern Great Plains, USA. The study area was in Cimarron County, Oklahoma, where land use is dominated by row-crop agriculture, livestock grazing, and grasslands enrolled in the Conservation Reserve Program (CRP). Ferruginous hawks (B. regalis) were uncommon and nested primarily in and around the Rita Blanca National Grassland. Swainson's hawks (B. swainsoni) were common and nested throughout the study area. Territories of ferruginous hawks contained more sandsage (Artemisia filifolia) habitat and less cropland and CRP lands than random sites, whereas territories of Swainson's hawks mirrored proportions of available landcover. Our results suggest that proportion of nearby sandsage habitat is an important factor in determining Swainson's hawk reproductive success. In addition, ferruginous hawk nest sites were located in areas that contained significantly more sandsage habitat than randomly selected sites in the study area. Nest-site availability may have constrained the distribution of buteos in our study area and is probably a major factor limiting nesting density of ferruginous hawks. Ferruginous hawks typically nest on man-made platforms (e. g., nest platforms and windmills) that in the study area were most common in and around the Rita Blanca National Grassland. Our results suggest that conversion of native grasslands to cropland may have negative consequences for ferruginous and Swainson's hawks. This relationship has been previously demonstrated in several studies of ferruginous hawks, but not for Swainson's hawks. In particular, loss of sandsage habitat on the southern Great Plains may have contributed to range declines in ferruginous hawks and decreased breeding success for Swainson's hawks. C1 [Wiggins, David A.; Schnell, Gary D.] Univ Oklahoma, Sam Noble Oklahoma Museum Nat Hist, Norman, OK 73072 USA. [Schnell, Gary D.] Univ Oklahoma, Dept Biol, Norman, OK 73072 USA. [Augustine, David J.] ARS, USDA, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. RP Schnell, GD (reprint author), 919 Yorkshire Dr, Breinigsville, PA 18031 USA. EM gschnell@ou.edu NR 46 TC 2 Z9 2 U1 3 U2 5 PU SOUTHWESTERN ASSOC NATURALISTS PI SAN MARCOS PA SOUTHWEST TEXAS STATE UNIV, DEPT BIOLOGY, 601 UNIVERSITY DR, SAN MARCOS, TX 78666 USA SN 0038-4909 EI 1943-6262 J9 SOUTHWEST NAT JI Southw. Natural. PD SEP PY 2014 VL 59 IS 3 BP 356 EP 363 PG 8 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA V44FJ UT WOS:000209734600007 ER PT J AU Unlu, I Farajollahi, A Rochlin, I Crepeau, TN Strickman, D Gaugler, R AF Unlu, Isik Farajollahi, Ary. Rochlin, Ilia Crepeau, Taryn N. Strickman, Daniel Gaugler, Randy TI Differences in male-female ratios of Aedes albopictus (Diptera: Culicidae) following ultra-low volume adulticide applications SO ACTA TROPICA LA English DT Article DE Asian tiger mosquito; Male mosquito; BG-Sentinel trap; Population dynamics; New Jersey ID STERILE INSECT TECHNIQUE; BG-SENTINEL; MOSQUITOS; TRAP; POPULATIONS; WOLBACHIA; SURVEILLANCE; MANAGEMENT; SURVIVAL; EFFICACY AB Suppression of Aedes albopictus populations is a-substantial challenge for mosquito control programs globally because juveniles of this species are found in numerous kinds of domestic artificial containers that are difficult to detect, access, and eliminate. We conducted a multi-year assessment of the effect of different interventions to control Ac. albopictus near the northernmost geographic boundary of the species in temperate North America and deployed an array of BG-Sentinel traps for adult surveillance. Here we present the results of a comparative examination of adult sex ratios in urban and suburban areas, shifts in sex ratios after control interventions, and a discussion of the critical drivers of population dynamics of Ae. albopictus in our area. We collected significantly more male mosquitoes in urban as compared to suburban areas in June through September, but not in May (p < 0.001). The higher number of male mosquitoes in urban areas could be attributed to a higher number of larval habitats within a closer proximity of the surveillance traps and the lower flight dispersal of males. Following application of adulticides in urban areas, Ac. albopictus male populations were reduced by 88% on average, which was higher than the 69% reduction in female populations. The higher reduction of male mosquitoes could be attributed to the smaller body mass of the males and their higher susceptibility to adulticides. The results of this study are directly relevant to the development of suitable control strategies that depend on manipulation of males, such as the sterile insect technique. The results could also be used to refine mosquito abatement by providing more accurate methods to determine the need and timing of vector control. Published by Elsevier B.V. C1 [Unlu, Isik; Farajollahi, Ary.; Rochlin, Ilia; Gaugler, Randy] Rutgers State Univ, Ctr Vector Biol, New Brunswick, NJ 08901 USA. [Unlu, Isik; Farajollahi, Ary.] Mercer Cty Mosquito Control, West Trenton, NJ 08628 USA. [Rochlin, Ilia] Suffolk Cty Vector Control, Yaphank, NY 11980 USA. [Crepeau, Taryn N.] Monmouth Cty Mosquito Exterminat Commiss, Tinton Falls, NJ 07724 USA. [Strickman, Daniel] USDA ARS, Off Natl Programs, Beltsville, MD 20782 USA. RP Unlu, I (reprint author), Mercer Cty Mosquito Control, 300 Scotch Rd, West Trenton, NJ 08628 USA. EM iunlu@mercercounty.org FU USDA Agricultural Research Service [USDA-ARS-58-6615-8-105]; Rutgers University FX We thank the Asian Tiger Mosquito Teams and county employees in Mercer and Monmouth Counties who assisted with surveillance and adulticiding. I would like to thank my mentor Dr. Dina Fonseca. for valuable discussions and input on earlier drafts. Thanks to the three anonymous reviewers selected by the editor. This work was funded in part by Cooperative Agreement USDA-ARS-58-6615-8-105 between the USDA Agricultural Research Service and Rutgers University entitled "Area-wide Management of the Asian Tiger Mosquito". NR 37 TC 7 Z9 7 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0001-706X EI 1873-6254 J9 ACTA TROP JI Acta Trop. PD SEP PY 2014 VL 137 BP 201 EP 205 DI 10.1016/j.actatropica.2014.05.009 PG 5 WC Parasitology; Tropical Medicine SC Parasitology; Tropical Medicine GA AM7AW UT WOS:000340018100026 PM 24907462 ER PT J AU Grayson, KL Mitchell, NJ Nelson, NJ AF Grayson, Kristine L. Mitchell, Nicola J. Nelson, Nicola J. TI A Threat to New Zealand's Tuatara Heats Up SO AMERICAN SCIENTIST LA English DT Article ID DEPENDENT SEX DETERMINATION; POPULATION; SPHENODON; RATIO C1 [Grayson, Kristine L.] Virginia Commonwealth Univ, USDA, Richmond, VA 23284 USA. [Mitchell, Nicola J.] Univ Western Australia, Sch Anim Biol, Nedlands, WA 6009, Australia. [Nelson, Nicola J.] Victoria Univ Wellington, Sch Biol Sci, Wellington, New Zealand. RP Grayson, KL (reprint author), Virginia Commonwealth Univ, USDA, Richmond, VA 23284 USA. EM kgrayson@richmond.edu RI Mitchell, Nicola/K-5277-2012 OI Mitchell, Nicola/0000-0003-0744-984X NR 15 TC 0 Z9 0 U1 1 U2 27 PU SIGMA XI-SCI RES SOC PI RES TRIANGLE PK PA PO BOX 13975, RES TRIANGLE PK, NC 27709 USA SN 0003-0996 EI 1545-2786 J9 AM SCI JI Am. Scientist PD SEP-OCT PY 2014 VL 102 IS 5 BP 350 EP 357 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM7DV UT WOS:000340025800015 ER PT J AU Thompson, ID Okabe, K Parrotta, JA Brockerhoff, E Jactel, H Forrester, DI Taki, H AF Thompson, Ian D. Okabe, Kimiko Parrotta, John A. Brockerhoff, Eckehard Jactel, Herve Forrester, David I. Taki, Hisatomo TI Biodiversity and ecosystem services: lessons from nature to improve management of planted forests for REDD-plus SO BIODIVERSITY AND CONSERVATION LA English DT Review DE Biodiversity; REDD-plus; Ecosystem services; Planted forest; Forest management; Plantation ID MIXED-SPECIES PLANTATIONS; HUMAN-MODIFIED LANDSCAPES; NITROGEN-FIXING TREES; TROPICAL RAIN-FOREST; SEED DISPERSAL; CLIMATE-CHANGE; FUNCTIONAL DIVERSITY; ASSOCIATIONAL RESISTANCE; BIRD PREDATION; ENVIRONMENTAL HETEROGENEITY AB Planted forests are increasingly contributing wood products and other ecosystem services at a global scale. These forests will be even more important as carbon markets develop and REDD-plus forest programs (forests used specifically to reduce atmospheric emissions of CO2 through deforestation and forest degradation) become common. Restoring degraded and deforested areas with long-rotation planted forests can be accomplished in a manner that enhances carbon storage and other key ecosystem services. Knowledge from natural systems and understanding the functioning novel of ecosystems can be instructive for planning and restoring future forests. Here we summarize information pertaining to the mechanisms by which biodiversity functions to provide ecosystem services including: production, pest control, pollination, resilience, nutrient cycling, seed dispersal, and water quality and quantity and suggest options to improve planted forest management, especially for REDD-plus. C1 [Thompson, Ian D.] Canadian Forest Serv, Sault Ste Marie, ON P6A 2E5, Canada. [Okabe, Kimiko; Taki, Hisatomo] Forest & Forest Prod Res Inst, Tsukuba, Ibaraki 3058687, Japan. [Parrotta, John A.] US Forest Serv, Res & Dev, Washington, DC 20024 USA. [Brockerhoff, Eckehard] Scion New Zealand Forest Res Inst, Christchurch, New Zealand. [Jactel, Herve] INRA, BIOGECO, F-33610 Cestas, France. [Jactel, Herve] Univ Bordeaux, BIOGECO, UMR1202, F-33400 Talence, France. [Forrester, David I.] Univ Freiburg, Fac Environm & Nat Resources, D-79108 Freiburg, Germany. RP Thompson, ID (reprint author), Canadian Forest Serv, 1219 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM ian.thompson@nrcan.gc.ca RI Brockerhoff, Eckehard/C-1528-2009; Forrester, David/L-5351-2013 OI Brockerhoff, Eckehard/0000-0002-5962-3208; FU International Union of Forest Research Organisations (IUFRO) Task Force 24 on 'Biodiversity and Ecosystem Services' and the IUFRO 'Global Forest Expert Panel'; Japan Environment Research and Technology Development Fund [S9] FX Support for this paper was provided in part by the International Union of Forest Research Organisations (IUFRO) Task Force 24 on 'Biodiversity and Ecosystem Services' and the IUFRO 'Global Forest Expert Panel'. The participation of Kimiko Okabe and Hisatomo Taki was by the The Japan Environment Research and Technology Development Fund S9. NR 214 TC 13 Z9 15 U1 10 U2 158 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0960-3115 EI 1572-9710 J9 BIODIVERS CONSERV JI Biodivers. Conserv. PD SEP PY 2014 VL 23 IS 10 BP 2613 EP 2635 DI 10.1007/s10531-014-0736-0 PG 23 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM5YO UT WOS:000339937300013 ER PT J AU Klasson, KT Uchimiya, M Lima, IM AF Klasson, K. Thomas Uchimiya, Minori Lima, Isabel M. TI Uncovering surface area and micropores in almond shell biochars by rainwater wash SO CHEMOSPHERE LA English DT Article DE Biochar; Nitrogen isotherm; Surface area; Washing; Ash; Micropore analysis ID DUBININ-RADUSHKEVICH; ADSORPTION; CARBON; MANURE; SOIL; CONTAMINANTS; ADSORBENTS; SORPTION; MERCURY; VAPORS AB Biochars have been considered for adsorption of contaminants in soil and water, as well as conditioning and improving soil quality. Pore surface area is an important property of biochar. Biochars were created from shells of two almond varieties with different ash content. The pyrolysis was performed at 650 and 800 degrees C for 40-240 min. Significant surface areas developed at the higher temperature and at pyrolysis times of 120 min and longer. Washing the materials in synthetic rainwater removed ash and exposed additional surface area, particularly in small-diameter pores. When results from low-ash almond shell biochars were compared with high-ash almond shell biochars, it was found that the pore distribution was more uniform for the high-ash starting material and almost independent of pyrolysis time or washing. The result from the washing study is important as it suggested that adsorptive properties may change once biochars are exposed to rainwater. Published by Elsevier Ltd. C1 [Klasson, K. Thomas; Uchimiya, Minori; Lima, Isabel M.] Agr Res Serv, USDA, New Orleans, LA 70124 USA. RP Klasson, KT (reprint author), Agr Res Serv, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM thomas.klasson@ars.usda.gov OI Klasson, K. Thomas/0000-0003-3358-3081 NR 33 TC 11 Z9 11 U1 8 U2 43 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0045-6535 EI 1879-1298 J9 CHEMOSPHERE JI Chemosphere PD SEP PY 2014 VL 111 BP 129 EP 134 DI 10.1016/j.chemosphere.2014.03.065 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA AM1JG UT WOS:000339601600018 ER PT J AU Ahmadi, M Arabi, M Ascough, JC Fontane, DG Engel, BA AF Ahmadi, Mehdi Arabi, Mazdak Ascough, James C., II Fontane, Darrell G. Engel, Bernard A. TI Toward improved calibration of watershed models: Multisite multiobjective measures of information SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Parameter estimation; Hydrologic modeling; Optimization; SWAT model; Water quality ID RAINFALL-RUNOFF MODELS; FORECASTING UNCERTAINTY ASSESSMENT; GLUE METHODOLOGY; AUTOMATIC CALIBRATION; PARAMETER-ESTIMATION; SENSITIVITY-ANALYSIS; HYDROLOGIC-MODELS; CATCHMENT MODELS; IMPACT; OPTIMIZATION AB This paper presents a computational framework for incorporation of disparate information from observed hydrologic responses at multiple locations into the calibration of watershed models. The framework consists of three components: 1) an a-priori characterization of system behavior; 2) a formal and statistically valid formulation of objective function(s) of model errors; and 3) an optimization engine to determine the Pareto-optimal front for the selected objectives. The proposed framework was applied for calibration of the Soil and Water Assessment Tool (SWAT) in the Eagle Creek Watershed, Indiana, USA using three single objective optimization methods [Shuffled Complex Evolution (SCE), Dynamically Dimensioned Search (DDS), and DiffeRential Evolution Adaptive Metropolis (DREAM)I, and one multi-objective optimization method. Solutions were classified into behavioral and non-behavioral using percent bias and Nash-Sutcliffe model efficiency coefficient. The results showed that aggregation of streamflow and NOx (NO3-N + NO2-N) information measured at multiple locations within the watershed into a single measure of weighted errors resulted in faster convergence to a solution with a lower overall objective function value than using multiple measures of information. However, the DREAM method solution was the only one among the three single objective optimization methods considered in this study that satisfied the conditions defined for characterizing system behavior. In particular, aggregation of streamflow and NOx responses undermined finding "very good" behavioral solutions for NOx, primarily because of the significantly larger number of observations for streamflow. Aggregation of only NOx responses into a single measure expedited finding better solutions although aggregation of data from nested sites appeared to be inappropriate because of correlated errors. This study demonstrates the importance of hydrologic and water quality data availability at multiple locations, and also highlights the use of multiobjective approaches for proper calibration of watershed models that are used for pollutant source identification and watershed management. Published by Elsevier Ltd. C1 [Ahmadi, Mehdi] Texas A&M Univ, Spatial Sci Lab, College Stn, TX 77843 USA. [Arabi, Mazdak; Fontane, Darrell G.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Ascough, James C., II] USDA ARS, ASRU, Ft Collins, CO 80526 USA. [Engel, Bernard A.] Purdue Univ, Dep Agr & Biol Engn, W Lafayette, IN 47907 USA. RP Ascough, JC (reprint author), USDA ARS, ASRU, 2150 Ctr Ave,Bldg D,Suite 200, Ft Collins, CO 80526 USA. EM ahmadi@tamu.edu; mazdak.arabi@colostate.edu; jim.ascough@ars.usda.gov; darrell.fontane@colostate.edu; engelb@purdue.edu FU U.S. Department of Agriculture; National Institute of Food and Agriculture [2007-51130-03876, 2009-51130-06038] FX This study was funded by U.S. Department of Agriculture and National Institute of Food and Agriculture grants 2007-51130-03876 and 2009-51130-06038. NR 77 TC 11 Z9 12 U1 3 U2 26 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD SEP PY 2014 VL 59 BP 135 EP 145 DI 10.1016/j.envsoft.2014.05.012 PG 11 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA AM2RZ UT WOS:000339699800011 ER PT J AU Ouyang, Y Zhang, JE Cui, LH AF Ouyang, Ying Zhang, Jia En Cui, Lihua TI Estimating impacts of land use on groundwater quality using trilinear analysis SO ENVIRONMENTAL MONITORING AND ASSESSMENT LA English DT Article DE Land use; Groundwater quality; Trilinear analysis ID NITRATE AB Groundwater is connected to the landscape above and is thus affected by the overlaying land uses. This study evaluated the impacts of land uses upon groundwater quality using trilinear analysis. Trilinear analysis is a display of experimental data in a triangular graph. Groundwater quality data collected from agricultural, septic tank, forest, and wastewater land uses for a 6-year period were used for the analysis. Results showed that among the three nitrogen species (i.e., nitrate and nitrite (NOx), dissolved organic nitrogen (DON), and total organic nitrogen (TON)), NOx had a high percentage and was a dominant species in the groundwater beneath the septic tank lands, whereas TON was a major species in groundwater beneath the forest lands. Among the three phosphorus species, namely the particulate phosphorus (PP), dissolved ortho phosphorus (PO (4) (3 -) ) and dissolved organic phosphorus (DOP), there was a high percentage of PP in the groundwater beneath the septic tank, forest, and agricultural lands. In general, Ca was a dominant cation in the groundwater beneath the septic tank lands, whereas Na was a dominant cation in the groundwater beneath the forest lands. For the three major anions (i.e., F-, Cl-, and SO (4) (2 -) ), F- accounted for < 1 % of the total anions in the groundwater beneath the forest, wastewater, and agricultural lands. Impacts of land uses on groundwater Cd and Cr distributions were not profound. This study suggests that trilinear analysis is a useful technique to characterize the relationship between land use and groundwater quality. C1 [Ouyang, Ying] US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, Mississippi State, MS 39762 USA. [Zhang, Jia En] South China Agr Univ, Dept Ecol, Guangzhou 510642, Guangdong, Peoples R China. [Cui, Lihua] South China Agr Univ, Coll Nat Resource & Environm, Guangzhou 510642, Guangdong, Peoples R China. RP Ouyang, Y (reprint author), US Forest Serv, USDA, Ctr Bottomland Hardwoods Res, 100 Stone Blvd,Thompson Hall,Room 309, Mississippi State, MS 39762 USA. EM youyang@fs.fed.us NR 23 TC 3 Z9 4 U1 0 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-6369 EI 1573-2959 J9 ENVIRON MONIT ASSESS JI Environ. Monit. Assess. PD SEP PY 2014 VL 186 IS 9 BP 5353 EP 5362 DI 10.1007/s10661-014-3784-8 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA AM4MB UT WOS:000339827500004 PM 24802588 ER PT J AU Tillman, PG AF Tillman, P. Glynn TI Physical barriers for suppression of movement of adult stink bugs into cotton SO JOURNAL OF PEST SCIENCE LA English DT Article DE Barrier wall; Sorghum sudangrass; Buckwheat; Nectar provision ID NEZARA-VIRIDULA HEMIPTERA; PENTATOMIDAE; HETEROPTERA; FARMSCAPES; DISPERSAL; TRENCH; PESTS; LEPIDOPTERA; PARASITOIDS; COLEOPTERA AB Nezara viridula (L.), Euschistus servus (Say), and Chinavia hilaris (Say) (Heteroptera: Pentatomidae) are economic pests of cotton in the southeastern USA. Because adult stink bugs exhibit edge-mediated dispersal at crop-to-crop interfaces as they colonize cotton, strategic placement of physical barriers at these interfaces could manage these pests. The objective of this study was to determine the effectiveness of a physical barrier, either synthetic or plant-based, at the peanut-to-cotton interface for suppressing stink bugs that would move to cotton. In 2012 and 2013, sorghum sudangrass (2.4 and 2.1 m high, respectively) was significantly taller than cotton (1.4 and 1.3 m high, respectively) which was taller than peanut (0.4 and 0.5 m high, respectively). Buckwheat (0.6 m high), planted only in 2012, was significantly taller than peanut, but shorter than cotton. For both years of the study, sorghum sudangrass and a 1.8-m-high polypropylene barrier wall effectively deterred dispersal of stink bugs into cotton. Because each of these barriers was taller than cotton, their success in protecting cotton likely was due to disruption of the flight of stink bugs from low-growing peanut into cotton. The shortest barrier wall (0.6-m-high) did not suppress stink bug dispersal into cotton probably because it was approximately the same height as peanut. In 2012, flowering buckwheat increased the efficacy of Trichopoda pennipes (F.) attacking N. viridula in cotton although it did not deter dispersal of stink bugs. In conclusion, a barrier at least as tall as cotton can effectively retard the entry of stink bug adults into cotton. C1 ARS, Crop Protect & Management Res Lab, USDA, Tifton, GA 31793 USA. RP Tillman, PG (reprint author), ARS, Crop Protect & Management Res Lab, USDA, POB 748, Tifton, GA 31793 USA. EM Glynn.Tillman@ars.usda.gov NR 38 TC 5 Z9 5 U1 3 U2 14 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 1612-4758 EI 1612-4766 J9 J PEST SCI JI J. Pest Sci. PD SEP PY 2014 VL 87 IS 3 BP 419 EP 427 DI 10.1007/s10340-014-0564-8 PG 9 WC Entomology SC Entomology GA AM5JB UT WOS:000339892400004 ER PT J AU Burnham, KK Sinnett, DR Johnson, JA Burnham, JL Baroch, JA Konkel, BW AF Burnham, K. K. Sinnett, D. R. Johnson, J. A. Burnham, J. L. Baroch, J. A. Konkel, B. W. TI New species records and changes in abundance of waterfowl in northwest Greenland SO POLAR BIOLOGY LA English DT Article DE Waterfowl; Ross's goose; Snow goose; Range expansion; Abundance ID GEESE BRANTA-CANADENSIS; GREATER SNOW GEESE; WEST GREENLAND; CANADA GEESE; CLIMATE-CHANGE; DISTRIBUTIONS; DYNAMICS; BIRDS; THULE AB Breeding populations of Nearctic and Palearctic waterfowl have undergone significant changes in abundance and distribution over the past 50 years. The Avanersuaq District in northwest Greenland is home to an assemblage of waterfowl from both geographic areas; however, minimal historic or current information is available on species abundance. In 2008 and 2009, we conducted field surveys in Greenland from 76.00A degrees to 77.35A degrees N for breeding and non-breeding waterfowl and have collected anecdotal field notes of avian observations over a 20-year period (1993-2012). During these periods, we documented the first observation of a Ross's goose (Chen rossii) and the first confirmed breeding by lesser snow geese (Chen caerulescens caerulescens) in Greenland. Northern pintails (Anas acuta) were observed for the first time in northwest Greenland, and a previously unknown breeding location for brent geese (Branta bernicla hrota) was also identified. Local populations of greater snow (C. c.) and Canada geese (B. canadensis) have increased in size. The Booth Sound and Drown Bay wetland areas and many islands throughout the Avanersuaq District were identified as critical habitat for both breeding and non-breeding waterfowl. Further increases in waterfowl abundance, including more frequent rare and new visitors, are likely in the study area as breeding populations further south continue to increase and an ameliorating climate allows for a longer breeding season. These results will prove useful as a baseline for comparisons with future surveys. C1 [Burnham, K. K.; Konkel, B. W.] High Arctic Inst, Orion, IL 61273 USA. [Sinnett, D. R.] Anim & Plant Hlth Inspect Serv, Natl Wildlife Dis Program, USDA, Wildlife Serv, Palmer, AK 99645 USA. [Johnson, J. A.] Univ N Texas, Inst Appl Sci, Dept Biol Sci, Denton, TX 76203 USA. [Burnham, J. L.] Augustana Coll, Dept Geog, Rock Isl, IL 61201 USA. [Baroch, J. A.] Anim & Plant Hlth Inspect Serv, Natl Wildlife Res Ctr, USDA, Wildlife Serv, Ft Collins, CO 80521 USA. RP Burnham, KK (reprint author), High Arctic Inst, 603 10th Ave, Orion, IL 61273 USA. EM kburnham@higharctic.org FU Offield Family Foundation; Wolf Creek Charitable Trust; United States Department of Agriculture; University of North Texas; Augustana College FX Thanks to Calen Offield, Jack Cafferty, Jack Stephens, and Jeremy Hughes who helped to collect data and conduct field surveys. Further thanks to Kim Diget, Kaj Kampp, and Knud Falk who contributed their unpublished field notes and observations. We thank the Greenland Home Rule Government for permits to work in Greenland, and the United States Air Force and Danish Liason Office for access to TAB. Further thanks to Polar Field Service, the 109th Air National Guard, the National Science Foundation, and the Bureau of Land Management for their assistance. Financial support for this project was provided by Calen Offield and the Offield Family Foundation, Wolf Creek Charitable Trust, United States Department of Agriculture, University of North Texas, Augustana College, Ruth O. Mutch, Yvon Chouinard, Patricia Burnham, Jack Stephens, Kim Pelle, and the many others who have donated to the High Arctic Institute. NR 63 TC 0 Z9 0 U1 1 U2 19 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0722-4060 EI 1432-2056 J9 POLAR BIOL JI Polar Biol. PD SEP PY 2014 VL 37 IS 9 BP 1289 EP 1300 DI 10.1007/s00300-014-1520-z PG 12 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AM5JK UT WOS:000339893400006 ER PT J AU Erickson, HE Perakis, SS AF Erickson, Heather E. Perakis, Steven S. TI Soil fluxes of methane, nitrous oxide, and nitric oxide from aggrading forests in coastal Oregon SO SOIL BIOLOGY & BIOCHEMISTRY LA English DT Article DE Coniferous forest; Methane uptake; Nitric oxide; Nitrous oxide; Red alder; Greenhouse gases ID GREENHOUSE-GAS FLUXES; TEMPERATE FOREST; DOUGLAS-FIR; RED ALDER; ATMOSPHERE EXCHANGE; PINUS-RADIATA; ADJACENT CONIFER; CLIMATE-CHANGE; CH4 OXIDATION; SPRUCE FOREST AB Soil exchanges of greenhouse and other gases are poorly known for Pacific Northwest forests where gradients in nutrient availability and soil moisture may contribute to large variations in fluxes. Here we report fluxes of methane (CH4), nitrous oxide (N2O), and nitric oxide (NO) over multiple seasons from three naturally N-rich, aggrading forests of coastal Oregon, USA. Mean methane uptake rates (3.2 mg CH4 m(-2) d(-1)) were high compared with forests globally, negatively related to water-filled pore space (WFPS), but unrelated to N availability or temperature. Emissions of NO (6.0 mu g NO-N m(-2) h(-1)) exceeded N2O (1.4 mu g N2O-N m(-2) h(-1)), except when WFPS surpassed 55%. Spatial variation in NO fluxes correlated positively with soil nitrate concentrations (which generally exceeded ammonium concentrations, indicating the overall high N status for the sites) and negatively with soil pH, and at one site increased with basal area of N-2-fixing red alder. Combined NO and N2O emissions were greatest from the site with highest annual net N mineralization and lowest needle litterfall C/N. Our findings of high CH4 uptake and NO/N2O ratios generally >1 most likely reflect the high porosity of the andic soils underlying the widespread regenerating forests in this seasonally wet region. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Erickson, Heather E.] USDA, Forest Serv, Pacific Northwest Res Stn, Portland Forestry Sci Lab, Portland, OR 97205 USA. [Perakis, Steven S.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. RP Erickson, HE (reprint author), USDA, Forest Serv, Pacific Northwest Res Stn, Portland Forestry Sci Lab, 620 SW Main,Suite 400, Portland, OR 97205 USA. EM ericicsonheather@yahoo.com; sperakis@usgs.gov FU USGS Forest and Rangeland Ecosystem Science Center; USFS PNW Research Station FX We thank Bridget Deemer and John A. Harrison for assistance and use of the GC at Washington State University in Vancouver, Chris Catricala at the USGS in Corvallis for field support, logistics, soil analyses and always knowing where everything was, and students and technicians from the Perakis lab for invaluable assistance in the field and lab. Sharon Hall, Michael Keller and Eric Davidson are also thanked for sharing ideas on configurations of the NO analyzer. Jennifer Davis and Joseph von Fischer gave feedback on an early version. Funding for this work was provided by the USGS Forest and Rangeland Ecosystem Science Center and the USFS PNW Research Station. NR 71 TC 4 Z9 4 U1 6 U2 49 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0038-0717 J9 SOIL BIOL BIOCHEM JI Soil Biol. Biochem. PD SEP PY 2014 VL 76 BP 268 EP 277 DI 10.1016/j.soilbio.2014.05.024 PG 10 WC Soil Science SC Agriculture GA AM2QM UT WOS:000339695900033 ER PT J AU Hopper, KR Diers, BW AF Hopper, Keith R. Diers, Brian W. TI Parasitism of soybean aphid by Aphelinus species on soybean susceptible versus resistant to the aphid SO BIOLOGICAL CONTROL LA English DT Article DE Host plant resistance; Biological control; Soybean; Aphis glycines; Aphelinus glycinis; Aphelinus certus ID COMMUNIS HYMENOPTERA-BRACONIDAE; HEMIPTERA APHIDIDAE; COLEMANI HYMENOPTERA; BIOLOGICAL-CONTROL; NORTH-AMERICA; MANAGEMENT; RAG1; REGISTRATION; PERFORMANCE; MATURATION AB The soybean aphid, Aphis glycines, is native to Asia, but during the last decade it has invaded North America, where it has spread to most soybean growing regions and become the most important insect pest of soybean. Current control of soybean aphid relies primarily on insecticides, but alternatives to insecticidal control are being explored, especially host plant resistance and biological control, which may interact positively or negatively. Research on host plant resistance to the soybean aphid has revealed six genes that affect resistance. We measured the impact of the two most studied resistance loci, Rag1 and Rag2, on two parasitoid species: Aphelinus glycinis, a recently described species from Asia, which is being introduced into the USA to control the soybean aphid, and Aphelinus certus, also from Asia but accidentally introduced into the USA. Resistance did not affect oviposition by either parasitoid species. However, resistance did reduce successful parasitism by A. glycinis, with each resistance allele causing a two-fold reduction in number of mummified aphids. The resistance alleles did not affect adult emergence, sex ratio, or the size of A. glycinis. For A. certus, the Rag1 resistance allele had no effect on parasitism, while the Rag2 resistance allele reduced parasitism four-fold. On the other hand, the Rag1 resistance allele increased the frequency of males among progeny and decreased female size of A. certus. Despite the reduction in parasitism, these parasitoids are nonetheless able to parasitize the soybean aphid on resistant soybean, which means that they should still contribute to the management of soybean aphid on resistant varieties. Published by Elsevier Inc. C1 [Hopper, Keith R.] USDA ARS, BIIR, Newark, DE 19713 USA. [Diers, Brian W.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. RP Hopper, KR (reprint author), USDA ARS, BIIR, 501 S Chapel St, Newark, DE 19713 USA. EM Keith.Hopper@ars.usda.gov FU North Central Soybean Research Program FX We thank Kathryn Lanier, Joshua Rhoades, Robert Kondos for assistance with insect rearing and conducting the experiments. This research was supported in part by a Grant from the North Central Soybean Research Program. NR 35 TC 2 Z9 3 U1 4 U2 29 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD SEP PY 2014 VL 76 BP 101 EP 106 DI 10.1016/j.biocontrol.2014.05.006 PG 6 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AM0MD UT WOS:000339538800014 ER PT J AU Berner, D Lagopodi, AL Kashefi, J Mukhina, Z Kolomiets, T Pankratova, L Kassanelly, D Cavin, C Smallwood, E AF Berner, Dana Lagopodi, Anastasia L. Kashefi, Javid Mukhina, Zhanna Kolomiets, Tamara Pankratova, Lyubov Kassanelly, Domenique Cavin, Craig Smallwood, Emily TI Field assessment, in Greece and Russia, of the facultative saprophytic fungus, Colletotrichum salsolae, for biological control of Russian thistle (Salsola tragus) SO BIOLOGICAL CONTROL LA English DT Article DE Biological control; Colletotrichum gloeosporioides; Colletotrichum salsolae; Russian thistle; Salsola tragus; Tumbleweed ID WEED-CONTROL; HOST-RANGE; GLOEOSPORIOIDES; AESCHYNOMENE; ANTHRACNOSE; PATHOGENS; IBERICA; SPREAD; WHEAT AB Russian thistle (Salsola tragus, tumbleweed, RT) is a problematic invasive weed in the United States (U.S.) and is a target of biological control efforts. The facultative saprophytic fungus Colletotrichum salsolae (CS) kills RT plants in greenhouse tests and is specific to Salsola spp., which are not native in the U.S. However, the effectiveness of CS in controlling RT has not been previously demonstrated. The objectives of this study were to determine in field tests: (1) disease progress of CS in time; (2) the relationship of disease progress to rainfall and temperature; (3) the effect of CS on RT plant density. Field tests were established in Serres and Kozani, Greece and Taman and Tuzla, Russia with isolates of the pathogen collected in the respective countries. Solid inoculum was prepared by asceptically inoculating sterile mixtures of grain and grain hulls with axenic cultures of CS. Spore suspensions used in Russia were prepared by blending pure sporulating cultures of CS with distilled water and diluting the suspension to 10(6) conidia per ml. Six field plots, each subdivided into 36 subplots, of an RT infested field in Serres, Greece were inoculated on October 23, 2006 by placing about 300 g of solid inoculum in the center of each plot. Four field plots, similarly subdivided, in each of two fields at Kozani, Greece were inoculated in the same way on October 1, 2010. RT density was counted and recorded in each sub-plot prior to inoculation and in September in each of 2 years following inoculation. Disease incidence and/or severity in each sub-plot were recorded at about 7-days intervals after inoculation. Rainfall and temperature data, from inoculation until 40-55 days after inoculation, were collected and recorded at Serres and at the Kozani airport meteorological station. Disease progressed rapidly at both sites and was correlated with cumulative rainfall. By 2 years after inoculation, RT had been eliminated from the Serres site and one field in Kozani. In the other Kozani field, RT density declined to 0-25% from original densities of about 80% in large areas of the field. RT plants in Taman and Tuzla, Russia were inoculated either with 250 g of grain inoculum or with a suspension of 10(6) conidia sprayed onto each plant until runoff. The proportion of diseased tissue reached 1.0 by 55 days after inoculation in both sites. Non-inoculated plants that were near inoculated plants became diseased quickly and reached the same disease severity as inoculated plants. Disease severity was correlated with cumulative rainfall but not temperature. This pathogen and inoculation procedure offers a low-cost solution to RT infestations. Since CS is specific to Salsola spp., this effective biological control is also environmentally friendly. Published by Elsevier Inc. C1 [Berner, Dana; Cavin, Craig; Smallwood, Emily] USDA ARS, Foreign Dis Weed Sci Res Unit, Ft Detrick, MD 21702 USA. [Lagopodi, Anastasia L.] Aristotle Univ Thessaloniki, Sch Agr, GR-54124 Thessaloniki, Greece. [Kashefi, Javid] USDA ARS, European Biol Control Lab, GR-54623 Thessaloniki, Greece. [Mukhina, Zhanna] All Russia Res Inst Biol Means Plant Protect, Krasnodar 350039, Russia. [Kolomiets, Tamara; Pankratova, Lyubov] All Russia Res Phytopathol Inst, VNIIF, B Vazemy 143050, Moscow Region, Russia. [Kassanelly, Domenique] Kuban State Univ, Dept Bot, Krasnodar 350751, Russia. RP Berner, D (reprint author), USDA ARS, Foreign Dis Weed Sci Res Unit, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM dana.berner@ars.usda.gov FU International Science and Technology Center-USDA/ARS Office of International Research Programs [3289]; U.S. Department of State; USDA, ARS, European Biological Control Laboratory, Montpellier, France FX Research in Russia was supported by International Science and Technology Center grant #3289 funded by the USDA/ARS Office of International Research Programs and the U.S. Department of State. Research in Greece was supported by USDA, ARS, European Biological Control Laboratory, Montpellier, France. NR 33 TC 0 Z9 0 U1 5 U2 23 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 1049-9644 EI 1090-2112 J9 BIOL CONTROL JI Biol. Control PD SEP PY 2014 VL 76 BP 114 EP 123 DI 10.1016/j.biocontrol.2014.06.003 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AM0MD UT WOS:000339538800016 ER PT J AU Fang, L Guanlin, T Li, XJ Hairu, C Ruhui, L Li, F AF Fang, Liu Guanlin, Tan Li, Xiaojing Hairu, Chen Ruhui, Li Li, Fan TI Simultaneous detection of four causal agents of tobacco bushy top disease by a multiplex one-step RT-PCR SO JOURNAL OF VIROLOGICAL METHODS LA English DT Article DE Tobacco bushy top disease; TBTV; TVDV; Sat-TBTV; TVDVaRNA; Multiplex; RT-PCR ID VIRUS; IDENTIFICATION; POTATO; PLANTS; RNA AB Tobacco bushy top disease is a complex disease caused by mixed infection of Tobacco bushy top virus (TBTV), Tobacco vein distorting virus (TVDV), satellite RNA of TBTV (Sat-TBTV) and Tobacco vein distorting virus associate RNA (TVDVaRNA). A one-tube multiplex reverse transcription-PCR (RT-PCR) assay was developed for simultaneous detection of the four causal agents of the disease. Four pairs of specific primers based on the conserved regions of each of the four disease agents were used in the one-tube RT-PCR. The RT-PCR products consisted of fragments of 1049 base pairs (bp) for TBTV, 792 bp for TVDVaRNA, 598 bp for Sat-TBTV and 357 bp for TVDV, and their origins were confirmed by sequencing. Primer concentrations and cycling condition were optimized for the multiplex RT-PCR. The detection limit of the assay was up to 10(-4) dilution. The assay was evaluated using tobacco plants infected naturally with one to four target viruses, transmission vector of aphids and field samples collected from Yunnan, Hunan, and Guizhou province, China. The results show that the multiplex RT-PCR is reliable and sensitive as a simple, rapid and cost-effective method to detect these pathogens in tobacco and aphid. This assay will be useful for virus surveys when large numbers of samples are tested. (C) 2014 Elsevier B.V. All rights reserved. C1 [Ruhui, Li] USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. [Fang, Liu; Li, Xiaojing; Hairu, Chen; Li, Fan] Yunnan Agr Univ, Key Lab Agr Biodivers Pest Management, China Educ Minist, Kunming 650201, Peoples R China. [Fang, Liu] Yunnan Agr Univ, Coll Agr & Biotechnol, Kunming 650201, Peoples R China. [Guanlin, Tan] Yunnan Agr Univ, Modern Educ Technol Ctr, Kunming 650201, Peoples R China. RP Ruhui, L (reprint author), USDA ARS, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. EM Ruhui.Li@ars.usda.gov; fanli@ynau.edu.cn FU National Natural Science Foundation of China [30660101]; Yunnan Provincial Science and Technology Innovation Team Plan of China [2011HC005] FX This work was financially supported by the National Natural Science Foundation of China (30660101) and Yunnan Provincial Science and Technology Innovation Team Plan of China (2011HC005). NR 19 TC 0 Z9 0 U1 2 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD SEP 1 PY 2014 VL 205 BP 99 EP 103 DI 10.1016/j.jviromet.2014.05.003 PG 5 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA AL5AF UT WOS:000339145200016 ER PT J AU Wilson, WC Romito, M Jasperson, DC Weingartl, H Binepal, YS Maluleke, MR Wallace, DB Van Vuren, PJ Paweska, JT AF Wilson, William C. Romito, Marco Jasperson, Dane C. Weingartl, Hana Binepal, Yatinder S. Maluleke, Moabi R. Wallace, David B. Van Vuren, Petrus Jansen Paweska, Janusz T. TI Development of a Rift Valley fever real-time RT-PCR assay that can detect all three genome segments (vol 193, pg 426, 2014) SO JOURNAL OF VIROLOGICAL METHODS LA English DT Correction C1 [Wilson, William C.; Jasperson, Dane C.] USDA, Agr Res Serv, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. [Romito, Marco; Maluleke, Moabi R.] ARC Onderstepoort Vet Inst, ZA-0110 Onderstepoort, South Africa. [Weingartl, Hana] Canadian Food Inspect Agcy, Natl Ctr Foreign Anim Dis, Winnipeg, MB, Canada. [Binepal, Yatinder S.] Kenya Agr Res Inst, Ctr Biotechnol, Nairobi, Kenya. [Wallace, David B.] Univ Pretoria, Fac Vet, Dept Vet Trop Dis, ZA-0110 Onderstepoort, South Africa. [Van Vuren, Petrus Jansen; Paweska, Janusz T.] Natl Inst Communicable Dis, Ctr Emerging & Zoonot Dis, Div Natl Hlth Lab Serv, Special Viral Pathogens Lab, ZA-2131 Johannesburg, South Africa. RP Wilson, WC (reprint author), USDA, Agr Res Serv, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. EM William.wilson@ars.usda.gov NR 1 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0166-0934 EI 1879-0984 J9 J VIROL METHODS JI J. Virol. Methods PD SEP 1 PY 2014 VL 205 BP 124 EP 124 DI 10.1016/j.jviromet.2014.05.005 PG 1 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology GA AL5AF UT WOS:000339145200020 PM 27353823 ER PT J AU Welch, KD Lee, ST Panter, KE Gardner, DR Knoppel, EL Green, BT Hammond, CK Hammond, ZJ Pfister, JA AF Welch, K. D. Lee, S. T. Panter, K. E. Gardner, D. R. Knoppel, E. L. Green, B. T. Hammond, C. K. Hammond, Z. J. Pfister, J. A. TI Studies on the teratogenicity of anabasine in a rat model SO TOXICON LA English DT Article DE Rat; Teratogen; Anabasine; Nicotinic acetylcholine receptor; Plant toxins ID NICOTINIC ACETYLCHOLINE-RECEPTOR; PREGNANT GOAT MODEL; FETAL MOVEMENT; CLEFT-PALATE; STEREOSELECTIVE POTENCIES; DEVELOPMENTAL TOXICITY; RELATIVE TOXICITIES; PIPERIDINE; QUINOLIZIDINE; ENANTIOMERS AB A number of plant toxins have been shown to be teratogenic to livestock. The teratogenic action of some of these alkaloids is mediated by nicotinic acetylcholine receptors (nAChR). However, for many of these alkaloids it is difficult to obtain sufficient quantities of individual alkaloids to perform teratology studies in livestock species. Therefore the objective of this study was to determine if a rat model can be utilized to characterize the teratogenic nature of individual plant toxins that are nAChR agonists. In this study, we evaluated the teratogenicity of anabasine by feeding pregnant rats anabasine-containing rodent chow from gestational day (GD) 6-21. On GD21, the dams were euthanized and the gravid uteri were removed. The gravid uteri and individual pups were weighed. The pups were evaluated for bone malformations including cleft palate and scoliosis. Overall, the results of this study suggest that the rat is not a good model to study the teratogenicity of plant toxins that are nAChR agonists. It is possible that in the rat model, anabasine administered orally via the chow may not result in sufficient reduction in fetal movement to cause the significant malformations observed in livestock species. Published by Elsevier Ltd. C1 [Welch, K. D.; Lee, S. T.; Panter, K. E.; Gardner, D. R.; Knoppel, E. L.; Green, B. T.; Hammond, C. K.; Hammond, Z. J.; Pfister, J. A.] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Welch, KD (reprint author), USDA ARS, Poisonous Plant Res Lab, 1150 E 1400 N, Logan, UT 84341 USA. EM Kevin.Welch@ars.usda.gov NR 27 TC 0 Z9 0 U1 2 U2 10 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD SEP PY 2014 VL 87 BP 32 EP 37 DI 10.1016/j.toxicon.2014.05.018 PG 6 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA AL5AM UT WOS:000339145900005 PM 24905648 ER PT J AU Suttles, SA Tyner, WE Shively, G Sands, RD Sohngen, B AF Suttles, Shellye A. Tyner, Wallace E. Shively, Gerald Sands, Ronald D. Sohngen, Brent TI Economic effects of bioenergy policy in the United States and Europe: A general equilibrium approach focusing on forest biomass SO RENEWABLE ENERGY LA English DT Article DE bioenergy; biofuel; climate change; computable general equilibrium; forestry; greenhouse gas (GHG) AB Renewable energy is an option for many countries simultaneously seeking to reduce dependence on imported petroleum and to reduce greenhouse gas (GHG) emissions that contribute to climate change. Forestry can play a role in environmental policies, such as renewable portfolio standards for bioelectricity, renewable fuel standards for biofuels, and forest carbon sequestration. This paper models interactions and interdependencies between bioelectricity and biofuel production, particularly from forest biomass. A global computable general equilibrium (CGE) model is used to measure the economic effects of bioenergy production from forest products, forest residues, and dedicated energy crops. The land use and emissions impacts on the global economy of revenue-neutral GHG mitigation policies are evaluated. Results show that mandated bioenergy production can substantially reduce carbon dioxide (CO2) emissions, especially through fossil fuel substitution in the electricity sector. Although emissions reductions from bioenergy production in the transportation fuel sector are less dramatic than those in the electricity sector, biofuels also have lower emissions rates than petroleum-based transportation fuels. Published by Elsevier Ltd. C1 [Suttles, Shellye A.; Sands, Ronald D.] USDA, Econ Res Serv, Washington, DC 20250 USA. [Tyner, Wallace E.; Shively, Gerald] Purdue Univ, Dept Agr Econ, W Lafayette, IN 47907 USA. [Shively, Gerald] Norwegian Univ Life Sci, Sch Business & Econ, As, Norway. [Sohngen, Brent] Ohio State Univ, Dept Agr Environm & Dev Econ, Columbus, OH 43210 USA. RP Suttles, SA (reprint author), USDA, Econ Res Serv, 1400 Independence Ave SW,Mail Stop 1800, Washington, DC 20250 USA. EM sasuttles@ers.usda.gov; wtyner@purdue.edu; shivelyg@purdue.edu; rsands@ers.usda.gov; sohngen.1@osu.edu OI Tyner, Wallace/0000-0003-1866-7025 FU United States Department of Agriculture; National Needs Fellowship in the Economics of Alternative Energy FX United States Department of Agriculture and National Needs Fellowship in the Economics of Alternative Energy. NR 42 TC 7 Z9 7 U1 2 U2 38 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-1481 J9 RENEW ENERG JI Renew. Energy PD SEP PY 2014 VL 69 BP 428 EP 436 DI 10.1016/j.renene.2014.03.067 PG 9 WC GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Energy & Fuels SC Science & Technology - Other Topics; Energy & Fuels GA AL0FI UT WOS:000338802400047 ER PT J AU Kong, QL Wu, AZ Qi, WY Qi, RD Carter, JM Rasooly, R He, XH AF Kong, Qiulian Wu, Aizhong Qi, Wenyuan Qi, Rongdi Carter, John Mark Rasooly, Reuven He, Xiaohua TI Effects of electron-beam irradiation on blueberries inoculated with Escherichia coli and their nutritional quality and shelf life SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Antioxidant activity; Blueberry; E. coli K12; Electron beam irradiation; L-Ascorbic acid; Total monomeric anthocyanins ID VACCINIUM-CORYMBOSUM L.; GAMMA-IRRADIATION; MICROBIOLOGICAL QUALITY; MICROBIAL SAFETY; FRESH PRODUCE; VITAMIN-C; STORAGE; SPINACH; FRUITS; FOOD AB Fresh blueberries have become a popular new functional food because of their remarkably high levels of antioxidant phytonutrients and health benefits. However, the potential prevalence of human pathogens on blueberries has become an increased concern because they are consumed fresh. Procedures effective in decontamination and extending shelf life without affecting fruit quality are needed. Electron-beam irradiation was applied to fresh blueberries at the doses ranging from 0.5 to 3.0 kGy and its effectiveness for inactivating Escherichia coli (E. coli) K-12 and extending shelf life were investigated. The decimal reduction dose, D-10 values, of E. coli in cultural medium and blueberries were 0.43 +/- 0.01 kGy and 0.37 +/- 0.015 kGy, respectively. Irradiation reduced bacteria inoculated on blueberries from 7.7 x 10(8) CFU/g to 6 CFU/g at 3.13 kGy and decreased the decaying of blueberries stored at 4 degrees C up to 72% and at room temperature up to 70% at this dose. No significant effect on the total monomeric anthocyanins, antioxidant activity, and ascorbic acid content of blueberries was observed from irradiation at doses <= 3 kGy. However, significant decreases in the antioxidant activity and L-ascorbic acid content were found in both control and irradiated blueberries after storage at 4 degrees C for 7 and 15 d. Information obtained in this study indicates that low dose electron-beam irradiation is effective in reducing E. coli and extending shelf life while maintaining the antioxidant properties of blueberries. Published by Elsevier B.V. C1 [Kong, Qiulian; Wu, Aizhong; Qi, Wenyuan; Qi, Rongdi] Shanghai Acad Agr Sci, Shanghai Shuneng Irradiat Technol Co Ltd, Shanghai 201400, Peoples R China. [Carter, John Mark; Rasooly, Reuven; He, Xiaohua] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP He, XH (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM sunny0123@vip.163.com; xiaohua.he@ars.usda.gov RI Carter, John Mark/K-2485-2015 OI Carter, John Mark/0000-0001-8251-4168 FU Science and Technology Bureau of Shanghai [12390700700, 11dz0502800]; USDA-ARS National Program [NP108]; CRIS project [5325-42000-048-00D] FX This research was supported by the projects of the Science and Technology Bureau of Shanghai project numbers 12390700700, 11dz0502800 and USDA-ARS National Program NP108, CRIS project 5325-42000-048-00D. The U.S. Department of Agriculture is an equal opportunity provider and employer. NR 42 TC 1 Z9 3 U1 4 U2 37 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD SEP PY 2014 VL 95 BP 28 EP 35 DI 10.1016/j.postharvbio.2014.04.004 PG 8 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AK7LS UT WOS:000338610400005 ER PT J AU Yan, RX Mattheis, J Gurtler, J Sites, J Fan, XT AF Yan, Ruixiang Mattheis, James Gurtler, Joshua Sites, Joseph Fan, Xuetong TI UV-C inactivation of Escherichia coli and dose uniformity on apricot fruit in a commercial setting SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Ultraviolet C; E. coli; Dosimetry; Apricot; Commercial application AB A UV-C treatment system (two treatment chambers connected by an inclined belt to rotate apricots between chambers) was tested in a commercial setting. Escherichia coli ATCC 25922, used as a surrogate for E. coli 0157:H7 to determine the system's antimicrobial efficacy, was inoculated onto fruit surfaces at a population of 6.8 log CFU/fruit. UV-C dosage was evaluated by attaching film dosimeters to six fixed locations on each apricot. Results suggested that reduction of inoculated E. coli ATCC 25922 populations on the apricot fruit by UV-C treatment was small (only 0.5-0.7 logs). There were large variations in UV-C doses among varying apricot surface locations. Approximately 1/3 of apricots had individual surfaces receiving less than 0.2 kJ m(-2) UV-C exposure, even though fruit received, on average, more than 1 kJ m(-2). Low reductions of E. coli may be attributed, in part, to non-uniform UV-C exposure. This study demonstrates the need to use a fruit rotation device more capable of delivering uniform UV-C dosage to the surface of apricots for inactivating bacteria in a commercial setting. Published by Elsevier B.V. C1 [Yan, Ruixiang; Gurtler, Joshua; Sites, Joseph; Fan, Xuetong] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Mattheis, James] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Yan, Ruixiang] Natl Engn & Technol Res Ctr Preservat Agr Prod, Tianjin Key Lab Postharvest Physiol & Storage Agr, Tianjin 300384, Peoples R China. RP Fan, XT (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM xuetong.fan@ars.usda.gov FU Center for Produce Safety [2012-212] FX The study was partially funded by a grant from the Center for Produce Safety (grant no. 2012-212). The authors thank Janie Countryman, Kim Sokorai, Robert Graham, and Rebecca Rivera for technical assistance, Dr. Robert Mandrell for providing E. coli. O157:H7, Drs. Gerald Sapers and Dave Geveke for reviewing the manuscript, and Mike Robinson and Kraig Massey for assistance with the UV-C system. NR 6 TC 1 Z9 1 U1 2 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD SEP PY 2014 VL 95 BP 46 EP 49 DI 10.1016/j.postharvbio.2014.04.005 PG 4 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AK7LS UT WOS:000338610400008 ER PT J AU Roon, DA Wipfli, MS Wurtz, TL AF Roon, David A. Wipfli, Mark S. Wurtz, Tricia L. TI Effects of invasive European bird cherry (Prunus padus) on leaf litter processing by aquatic invertebrate shredder communities in urban Alaskan streams SO HYDROBIOLOGIA LA English DT Article DE Invasive species; Riparian forests; Leaf litter processing; Aquatic invertebrate shredders; Prunus padus; Alaska ID RIPARIAN ZONES; HEADWATER STREAMS; MACROINVERTEBRATE COLONIZATION; SOUTHEASTERN ALASKA; NONNATIVE TAMARIX; JAPANESE KNOTWEED; ALNUS-TENUIFOLIA; BREAKDOWN RATES; NATIVE POPULUS; UNITED-STATES AB European bird cherry (Prunus padus) (EBC) is an invasive ornamental tree that is spreading rapidly in riparian forests of urban Alaska. To determine how the spread of EBC affects leaf litter processing by aquatic invertebrate shredders, we conducted complementary leaf pack experiments in two streams located in Anchorage, Alaska. The first experiment contrasted invasive EBC with three native tree species-thin-leaf alder (Alnus tenuifolia), paper birch (Betula neoalaskana), and black cottonwood (Populus trichocarpa)-in one reach of Chester Creek; finding that EBC leaf litter broke down significantly faster than birch and cottonwood, but at a similar rate to alder. The second experiment contrasted EBC with alder in four reaches of Campbell and Chester creeks; finding that while EBC leaf litter broke down significantly faster than alder in Chester Creek, EBC broke down at a similar rate to alder in Campbell Creek. Although EBC sometimes supported fewer shredders by both count and mass, shredder communities did not differ significantly between EBC and native plants. Collectively, these data suggest that invasive EBC is not currently exhibiting strong negative impacts on leaf litter processing in these streams, but could if it continues to spread and further displaces native species over time. C1 [Roon, David A.] Univ Alaska Fairbanks, Dept Biol & Wildlife, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Wipfli, Mark S.] Univ Alaska Fairbanks, Inst Arctic Biol, US Geol Survey, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. [Wurtz, Tricia L.] US Forest Serv, USDA, Forest Hlth Program, Fairbanks, AK 99709 USA. RP Roon, DA (reprint author), Univ Alaska Fairbanks, Dept Biol & Wildlife, Alaska Cooperat Fish & Wildlife Res Unit, Fairbanks, AK 99775 USA. EM dave.roon@gmail.com FU US Fish and Wildlife Service; USDA Forest Service; Alaska EPSCoR; Institute of Arctic Biology; University of Alaska Fairbanks FX Thanks to the US Fish and Wildlife Service, USDA Forest Service, Alaska EPSCoR, Institute of Arctic Biology and the University of Alaska Fairbanks for funding. We thank Dustin Merrigan, John Hill, Steve Ewest, Uinniq Ahgeak, and Thomas Robertson for assistance with field and laboratory work. Thanks to Dan Rinella and Dan Bogan at UAA for advice on the experimental design and providing summer lab space, to Arny Blanchard at University of Alaska Fairbanks for his assistance with the statistical analysis and Jim Kruse from the USDA Forest Service for providing an internal peer review of the manuscript. NR 61 TC 4 Z9 4 U1 0 U2 42 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0018-8158 EI 1573-5117 J9 HYDROBIOLOGIA JI Hydrobiologia PD SEP PY 2014 VL 736 IS 1 BP 17 EP 30 DI 10.1007/s10750-014-1881-x PG 14 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA AJ9KC UT WOS:000338027700002 ER PT J AU Haag, WR Williams, JD AF Haag, Wendell R. Williams, James D. TI Biodiversity on the brink: an assessment of conservation strategies for North American freshwater mussels SO HYDROBIOLOGIA LA English DT Article DE Restoration; Fragmentation; Extinction; Propagation; Unionoida ID TENNESSEE RIVER DRAINAGE; BIVALVIA UNIONIDAE; UNITED-STATES; LAMPSILIS-SILIQUOIDEA; DREISSENA-POLYMORPHA; CUMBERLAND RIVER; MICROHABITAT USE; SPECIES TRAITS; LIFE-HISTORY; POPULATIONS AB The North America freshwater mussel fauna has suffered an inordinately high recent extinction rate, and the small size and isolation of many remaining populations portends a continued diminishment of this fauna. Causes of extinction and imperilment are varied but revolve around massive habitat loss, deterioration, and fragmentation. The National Strategy for the Conservation of Native Mussels, published in 1997, has guided efforts to address this crisis. Considerable progress has been made toward several of the Strategies' goals, particularly increasing our knowledge of mussel biology, promoting mussel conservation, and development of techniques for captive mussel propagation. However, mussel conservation should focus more directly on reducing fragmentation through bold and aggressive habitat restoration. In addition to dam removal, improvement in dam tailwater flows, and restoration of channelized streams, identification of factors that eliminated mussels from many otherwise intact streams is critical. Translocation and captive propagation will be key elements in reestablishing mussel assemblages in restored habitats, but these techniques should be used with caution and primarily to increase the occurrence of a species throughout its historical range. Conserving mussel diversity in an ever-changing world is dependent on promoting the natural, long-term sustainability and evolutionary potential of mussel populations. C1 [Haag, Wendell R.] US Forest Serv, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. [Williams, James D.] Univ Florida, Florida Museum Nat Hist, Gainesville, FL 32611 USA. RP Haag, WR (reprint author), US Forest Serv, Ctr Bottomland Hardwoods Res, Oxford, MS 38655 USA. EM whaag@fs.fed.us NR 83 TC 24 Z9 24 U1 6 U2 91 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0018-8158 EI 1573-5117 J9 HYDROBIOLOGIA JI Hydrobiologia PD SEP PY 2014 VL 735 IS 1 BP 45 EP 60 DI 10.1007/s10750-013-1524-7 PG 16 WC Marine & Freshwater Biology SC Marine & Freshwater Biology GA AI6IV UT WOS:000336977600003 ER PT J AU Zhao, F Guo, YQ Huang, YB Reddy, KN Lee, MA Fletcher, RS Thomson, SJ AF Zhao, Feng Guo, Yiqing Huang, Yanbo Reddy, Krishna N. Lee, Matthew A. Fletcher, Reginald S. Thomson, Steven J. TI Early detection of crop injury from herbicide glyphosate by leaf biochemical parameter inversion SO INTERNATIONAL JOURNAL OF APPLIED EARTH OBSERVATION AND GEOINFORMATION LA English DT Article DE Crop injury; Glyphosate; Foliar biochemistry; Sensitivity analysis; Model inversion; Hyperspectral ID OPTICAL-PROPERTIES MODEL; PROSPECT; DRIFT; REFLECTANCE; INDICATORS AB Early detection of crop injury from herbicide glyphosate is of significant importance in crop management. In this paper, we attempt to detect glyphosate-induced crop injury by PROSPECT (leaf optical PROperty SPECTra model) inversion through leaf hyperspectral reflectance measurements for non-Glyphosate-Resistant (non-GR) soybean and non-GR cotton leaves. The PROSPECT model was inverted to retrieve chlorophyll content (Ca+b), equivalent water thickness (C-w), and leaf mass per area (C-m) from leaf hyperspectral reflectance spectra. The leaf stress conditions were then evaluated by examining the temporal variations of these biochemical constituents after glyphosate treatment. The approach was validated with greenhouse-measured datasets. Results indicated that the leaf injury caused by glyphosate treatments could be detected shortly after the spraying for both soybean and cotton by PROSPECT inversion, with Ca+b of the leaves treated with high dose solution decreasing more rapidly compared with leaves left untreated, whereas the Cw and Cm showed no obvious difference between treated and untreated leaves. For both non-GR soybean and non-GR cotton, the retrieved Ca+b values of the glyphosate treated plants from leaf hyperspectral data could be distinguished from that of the untreated plants within 48 h after the treatment, which could be employed as a useful indicator for glyphosate injury detection. These findings demonstrate the feasibility of applying the PROSPECT inversion technique for the early detection of leaf injury from glyphosate and its potential for agricultural plant status monitoring. (C) 2014 Elsevier B.V. All rights reserved. C1 [Zhao, Feng; Guo, Yiqing] Beihang Univ, Sch Instrumentat Sci & Optoelect Engn, Beijing 100191, Peoples R China. [Huang, Yanbo; Reddy, Krishna N.; Lee, Matthew A.; Fletcher, Reginald S.; Thomson, Steven J.] USDA ARS, Crop Prod Syst Res Unit, Stoneville, MS 38776 USA. RP Zhao, F (reprint author), Beihang Univ, Sch Instrumentat Sci & Optoelect Engn, Beijing 100191, Peoples R China. EM zhaofeng@buaa.edu.cn FU Chinese Natural Science Foundation [41371325, 40901156]; Civil Aerospace Technology Pre-research Project of China [D040201-03]; State Key Laboratory of Remote Sensing Science [2009kfjj022] FX This work was supported by Chinese Natural Science Foundation under Project 41371325 and 40901156, the Civil Aerospace Technology Pre-research Project of China (Grant No. D040201-03), and the State Key Laboratory of Remote Sensing Science under Project 2009kfjj022. We thank Efren Ford and Paige Goodlett for technical assistance. NR 28 TC 9 Z9 9 U1 5 U2 29 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0303-2434 J9 INT J APPL EARTH OBS JI Int. J. Appl. Earth Obs. Geoinf. PD SEP PY 2014 VL 31 BP 78 EP 85 DI 10.1016/j.jag.2014.03.010 PG 8 WC Remote Sensing SC Remote Sensing GA AI2PQ UT WOS:000336700900008 ER PT J AU Lee, MS Hwang, YS Lee, J Choung, MG AF Lee, Min-Seuk Hwang, Young-Sun Lee, Jinwook Choung, Myoung-Gun TI The characterization of caffeine and nine individual catechins in the leaves of green tea (Camellia sinensis L.) by near-infrared reflectance spectroscopy SO FOOD CHEMISTRY LA English DT Article DE Green tea; Near-infrared reflectance spectroscopy; Caffeine; Catechins; High performance liquid chromatography ID PERFORMANCE LIQUID-CHROMATOGRAPHY; FT-NIR SPECTROSCOPY; NONDESTRUCTIVE DETERMINATION; CALIBRATION PROCEDURES; QUANTITATIVE-ANALYSIS; TOTAL POLYPHENOLS; CANCER; HPLC; FLAVONOIDS; ALKALOIDS AB Near-infrared reflectance spectroscopy (NIRS) was used to determine the contents of caffeine and nine individual catechins in tea leaves. A total of 665 samples were scanned by NIRS, and also by high performance liquid chromatography coupled to a diode array detector to determine the contents of caffeine and nine individual catechins. The calibration models for caffeine, EGC, C, EGCG, EC, ECG, and total catechins had high r(2) (more than 0.90) and RSP (the ratio of standard deviation of reference data to SEP(C) in the external validation set) values (more than 4.1), indicating a good correlation between reference values and NIRS predicted values. In contrast, the calibration models of GC and EGCG-3Me had low r(2) and RSP values (below 0.8 and 2.0). Therefore, these results suggest that NIRS could be applied for the rapid determination of the contents of caffeine, EGC, C, EGCG, EC, ECG, and total catechins in tea leaves for breeding programs that develop high-quality tea plants. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Lee, Min-Seuk] Jangwon Co LTD, Sulloc Cha R&D Ctr, Seogwipo 699920, Jeju, South Korea. [Hwang, Young-Sun; Choung, Myoung-Gun] Kangwon Natl Univ, Dept Herbal Med Resource, Dogye Up 245907, Samcheok, South Korea. [Lee, Jinwook] USDA ARS, Tree Fruit Res Lab, Wenatchee, WA 98801 USA. [Choung, Myoung-Gun] Univ Texas Arlington, Dept Biol, Arlington, TX 76019 USA. RP Choung, MG (reprint author), Kangwon Natl Univ, Dept Herbal Med Resource, Dogye Campus,Hwangjori 3, Dogye Up 245907, Samcheok, South Korea. EM cmg7004@kangwon.ac.kr NR 43 TC 13 Z9 16 U1 10 U2 120 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD SEP 1 PY 2014 VL 158 BP 351 EP 357 DI 10.1016/j.foodchem.2014.02.127 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AH0JQ UT WOS:000335805600049 PM 24731354 ER PT J AU Assoi, S Konan, K Walker, LT Holser, R Agbo, GN Dodo, H Wicker, L AF Assoi, Sylvie Konan, Koffi Walker, Lloyd T. Holser, Ron Agbo, Georges N. Dodo, Hortense Wicker, Louise TI Functionality and yield of pectin extracted from Palmyra palm (Borassus aethiopum Mart) fruit SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Palmyra palm; Pectin; Galacturonic acid; Gelling; Viscosity ID RHEOLOGICAL PROPERTIES; EXPERIMENTAL-DESIGN; METHOXYL PECTIN; SUGAR-BEET; PEELS; ESTERIFICATION; SUBSTANCES; PRODUCTS; BEHAVIOR; GEL AB Large numbers of fruits from Palmyra palm (Borassus aethiopum Mart), a common, native tree of tropical regions, are underutilized and left to spoil. This work was conducted to assess pectin content of Palmyra palm fruit, and to develop a reliable protocol for pectin extraction. Yield and galacturonic acid content of Palmyra palm fruit soluble solids were investigated at various pH (natural pH of 5.2-5.5, 2.5, 7), time (30-120 min) and temperature (70 degrees C, 80 degrees C, 90 degrees C). Yield and galacturonic acid content of extracted soluble solids were strongly dependent (p <0.0001) on temperature, time and pH of extraction and ranged from 47 to 149 g kg(-1) and 682 to 880 g kg(-1) dry weight, respectively. High galacturonic acid (808-852 g kg(-1)) were obtained at 70 degrees C and 90 degrees C under natural pH; at 70 degrees C pH 7 and pH 2.5 at all temperatures studied. Analysis of pectin extracted at natural pH (30 mm, 90 degrees C) revealed highly esterified pectin with good gelling and emulsifying properties. Production of galacturonic acid of more than 650 g kg(-1) dry weight indicates that Palmyra palm fruit could serve as an excellent raw material for industrial pectin production. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Assoi, Sylvie; Agbo, Georges N.] Univ Cocody, Dept Biochimie & Sci Aliments, UFR Biosci, Abidjan 22, Cote Ivoire. [Wicker, Louise] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. [Wicker, Louise] Korea Univ, Coll Educ, Dept Home Econ Educ, Seoul 136701, South Korea. [Konan, Koffi; Walker, Lloyd T.; Dodo, Hortense] Alabama A&M Univ, Dept Food & Anim Sci, Normal, AL 35762 USA. [Holser, Ron] USDA ARS, SAA, Qual & Safety Assessment Unit, Athens, GA 30605 USA. RP Wicker, L (reprint author), Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. EM lwicker@uga.edu NR 53 TC 2 Z9 2 U1 4 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD SEP PY 2014 VL 58 IS 1 BP 214 EP 221 DI 10.1016/j.lwt.2014.02.019 PG 8 WC Food Science & Technology SC Food Science & Technology GA AG7SP UT WOS:000335619100028 ER PT J AU O'Connor, TM Chen, TA Rodriguez, BD Hughes, SO AF O'Connor, Teresia M. Chen, Tzu-An Rodriguez, Betty del Rio Hughes, Sheryl O. TI Psychometric validity of the parent's outcome expectations for children's television viewing (POETV) scale SO BMC PUBLIC HEALTH LA English DT Article ID BODY-MASS INDEX; SEXUAL-BEHAVIOR; SEDENTARY BEHAVIOR; PHYSICAL-ACTIVITY; RANDOMIZED-TRIAL; YOUNG-CHILDREN; COMPUTER USE; SCREEN TIME; MEDIA USE; OBESITY AB Background: TV and other screen use are common among elementary school aged children with both potential benefits and harms. It is not clear why some parents restrict their children's screen use and others do not. Parent's outcome expectations for allowing their child to watch TV and other screen media, i.e. the perceived 'costs' and 'benefits,' may be influential. Our objective was to develop a measure of Parent's Outcome Expectations for Children's TV Viewing (POETV) and test the psychometrics of the resulting instrument among parents with children 6-12 years old. Methods: An ethnically diverse sample (n = 311) of parents from Harris County, Texas completed measures for POETV, demographics, and parent and child TV viewing and other screen media use via an internet survey. The sample was randomly split and an exploratory factor analysis (EFA) was conducted among the first half of the sample separately for Positive and Negative POETV. A confirmatory factor analysis (CFA) assessed the fit of the resulting factors with the data in the second half of the sample. Internal reliabilities and Spearman partial correlations (controlling for confounders) of children's TV and other screen use with the resulting POETV factors were calculated for the full sample. Results: EFA identified two factors for Positive POETV (Parent Centered; Child Centered) and two factors for Negative POETV (TV & Content Exposure; Prevent Other Activities). Follow up CFA confirmed moderate to good psychometric properties for both factor structures with the addition of four correlated errors in the Positive POETV model. Internal reliabilities were appropriate (Cronbach's alpha >0.7). Parent Centered Positive POETV and Child Centered Positive POETV were correlated with children's TV viewing on weekdays (0.14, p < 0.05) and weekends (0.17, p < 0.01) respectively. Both also correlated with other screen media use on weekends (0.20 and 0.21, p < 0.001). Prevent Other Activities Negative POETV was negatively correlated with children's TV viewing on weekdays (-0.16, p < 0.01), weekends (-0.14, p < 0.05) and other screen media on weekends (-0.14, p < 0.05). Conclusions: The Positive and Negative POETV scales offer a new tool to better define predictors of screen media parenting practices and child screen media use behaviors. C1 [O'Connor, Teresia M.; Chen, Tzu-An; Hughes, Sheryl O.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. [O'Connor, Teresia M.; Rodriguez, Betty del Rio] Baylor Coll Med, Acad Gen Pediat, Dept Pediat, Houston, TX 77030 USA. RP O'Connor, TM (reprint author), USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX 77030 USA. EM teresiao@bcm.edu FU Texas Children's Hospital Pilot Grant FX This study was funded by a Texas Children's Hospital Pilot Grant NR 47 TC 2 Z9 2 U1 2 U2 16 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2458 J9 BMC PUBLIC HEALTH JI BMC Public Health PD AUG 31 PY 2014 VL 14 AR 894 DI 10.1186/471-2458-14-894 PG 8 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AO9BR UT WOS:000341651000001 PM 25175279 ER PT J AU Chintala, R Schumacher, TE Kumar, S Malo, DD Rice, JA Bleakley, B Chilom, G Clay, DE Julson, JL Papiernik, SK Gu, ZR AF Chintala, Rajesh Schumacher, Thomas E. Kumar, Sandeep Malo, Douglas D. Rice, James A. Bleakley, Bruce Chilom, Gabriela Clay, David E. Julson, James L. Papiernik, Sharon K. Gu, Zheng Rong TI Molecular characterization of biochars and their influence on microbiological properties of soil SO JOURNAL OF HAZARDOUS MATERIALS LA English DT Article DE FDA; Dehydrogenase; Protease; Aryl sulfatase; NMR; SEM ID BLACK CARBON; MICROBIAL ACTIVITIES; ACTIVATED CARBON; ORGANIC-MATTER; PINE FORESTS; CHARCOAL; SORPTION; MINERALIZATION; AMENDMENTS; COMMUNITY AB The tentative connection between the biochar surface chemical properties and their influence on microbially mediated mineralization of C, N, and S with the help of enzymes is not well established. This study was designed to investigate the effect of different biomass conversion processes (microwave pyrolysis, carbon optimized gasification, and fast pyrolysis using electricity) on the composition and surface chemistry of biochar materials produced from corn stover (Zea mays L.), switchgrass (Panicum virgatum L), and Ponderosa pine wood residue (Pinus ponderosa Lawson and C. Lawson) and determine the effect of biochars on mineralization of C, N, and S and associated soil enzymatic activities including esterase (fluorescein diacetate hydrolase, FDA), dehydrogenase (DHA), beta-glucosidase (GLU), protease (PROT), and aryl sulfatase (ARSUL) in two different soils collected from footslope (Brookings) and crest (Maddock) positions of a landscape. Chemical properties of biochar materials produced from different batches of gasification process were fairly consistent. Biochar materials were found to be highly hydrophobic (low H/C values) with high aromaticity, irrespective of biomass feedstock and pyrolytic process. The short term incubation study showed that biochar had negative effects on microbial activity (FDA and DHA) and some enzymes including beta-glucosidase and protease. Published by Elsevier B.V. C1 [Chintala, Rajesh; Schumacher, Thomas E.; Kumar, Sandeep; Malo, Douglas D.; Clay, David E.] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Rice, James A.; Chilom, Gabriela] S Dakota State Univ, Dept Chem & Biochem, Brookings, SD 57007 USA. [Bleakley, Bruce] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Julson, James L.; Gu, Zheng Rong] S Dakota State Univ, Dept Agr & Biosyst Engn, Brookings, SD 57007 USA. [Papiernik, Sharon K.] USDA ARS, Brookings, SD USA. RP Chintala, R (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM rajesh.chintala@sdstate.edu FU Agriculture and Food Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-67009-30076] FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67009-30076 from the USDA National Institute of Food and Agriculture. NR 86 TC 21 Z9 23 U1 10 U2 70 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3894 EI 1873-3336 J9 J HAZARD MATER JI J. Hazard. Mater. PD AUG 30 PY 2014 VL 279 BP 244 EP 256 DI 10.1016/j.jhazmat.2014.06.074 PG 13 WC Engineering, Environmental; Engineering, Civil; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AR2CB UT WOS:000343390100031 PM 25064262 ER PT J AU Thekke-Veetil, T Ho, T Keller, KE Martin, RR Tzanetakis, IE AF Thekke-Veetil, Thanuja Ho, Thien Keller, Karen E. Martin, Robert R. Tzanetakis, Ioannis E. TI A new ophiovirus is associated with blueberry mosaic disease SO VIRUS RESEARCH LA English DT Article DE Blueberry mosaic; Ophioviruses; Negative-strand RNA virus ID CITRUS-PSOROSIS-VIRUS; BIG-VEIN OPHIOVIRUS; STRUCTURE PREDICTION; COMPLEMENTARY STRAND; NUCLEOTIDE-SEQUENCE; NEGATIVE POLARITY; MOVEMENT PROTEIN; CONSERVED MOTIFS; RNA; POLYMERASE AB Blueberry mosaic disease (BMD) was first described more than 60 years ago and is caused by a yet unidentified graft transmissible agent. A combination of traditional methods and next generation sequencing disclosed the presence of a new ophiovirus in symptomatic plants. The virus was detected in all BMD samples collected from several production areas of North America and was thus named blueberry mosaic associated virus. Phylogenetic analysis, supported by high bootstrap values, places the virus within the family Ophioviridae. The genome organization resembles that of citrus psorosis virus, the type member of the genus Ophiovirus. The implications of this discovery in BMD control and blueberry virus certification schemes are also discussed. (C) 2014 Elsevier B.V. All rights reserved. C1 [Thekke-Veetil, Thanuja; Ho, Thien; Tzanetakis, Ioannis E.] Univ Arkansas Syst, Dept Plant Pathol, Div Agr, Fayetteville, AR 72701 USA. [Keller, Karen E.; Martin, Robert R.] USDA ARS, Corvallis, OR 97330 USA. RP Tzanetakis, IE (reprint author), Univ Arkansas Syst, Dept Plant Pathol, Div Agr, Fayetteville, AR 72701 USA. EM itzaneta@uark.edu RI Ho, Thien/A-7209-2012 OI Ho, Thien/0000-0003-0914-306X FU Southern Region Small Fruit Consortium [2011-13, 2012-07]; USDA-NCPN [12-8100-1572-CA] FX The authors acknowledge the funding provided by the Southern Region Small Fruit Consortium (Projects #2011-13 and 2012-07) and USDA-NCPN (12-8100-1572-CA) for this study. We thank Dr. M. Elena Garcia (University or Arkansas) and Annemiek Schilder (Michigan State University) for providing material used in the study. NR 45 TC 8 Z9 8 U1 1 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD AUG 30 PY 2014 VL 189 BP 92 EP 96 DI 10.1016/j.virusres.2014.05.019 PG 5 WC Virology SC Virology GA AO7UI UT WOS:000341557700015 PM 24874194 ER PT J AU Parinussa, RM Yilmaz, MT Anderson, MC Hain, CR de Jeu, RAM AF Parinussa, R. M. Yilmaz, M. T. Anderson, M. C. Hain, C. R. de Jeu, R. A. M. TI An intercomparison of remotely sensed soil moisture products at various spatial scales over the Iberian Peninsula SO HYDROLOGICAL PROCESSES LA English DT Article DE soil moisture; remote sensing; intercomparison; downscaling ID LAND DATA ASSIMILATION; AMSR-E; VALIDATION; ASCAT; SCATTEROMETER; RETRIEVALS; MODEL; RESOLUTION; TREND AB Soil moisture (SM) can be retrieved from active microwave (AM), passive microwave (PM) and thermal infrared (TIR) observations, each having unique spatial and temporal coverages. A limitation of TIR-based retrievals is a dependence on cloud-free conditions, whereas microwave retrievals are almost all weather proof. A downside of SM retrievals from PM is the coarse spatial resolution. Although SM retrievals at coarse spatial resolution proved to be valuable for global-scale and continental-scale studies, their value for regional-scale studies remains limited. To increase the use of SM retrievals from PM observations, an existing method to enhance their spatial resolution was applied. We present an intercomparison study over the Iberian Peninsula for three SM products on two different spatial sampling grids. The remotely sensed SM products were also compared with in situ observations from the Remedhus network. Variations between ground data and satellite-based SM are observed; all three remotely sensed SM products show good agreement to the ground observations. The comparison shows that these ground observations and satellite data are consistent, based on the correlation coefficient (R) and root mean square error (RMSE). The remotely sensed products were intercompared after sampling at 25 x 25 km(2) and after applying the smoothing filter-based intensity modulation (SFIM) downscaling technique at 10 x 10 km(2) grids. After the application of the SFIM technique, the SM retrievals from PM observations show better agreement with the other remotely sensed SM products for approximately 40% of the study area. For another 40% of the study area, we found a similar agreement between these product combinations, whereas in extreme environments, both arid and densely vegetated regions, the agreement decreases after the application of the SFIM technique. Agreement between retrievals of absolute SM content from PM and TIR observations is generally high (R = 0.77 for semi-arid areas). This study enhances our understanding of the remotely sensed SM products for improvements of SM retrieval and merging strategies. Copyright (C) 2013 John Wiley & Sons, Ltd. C1 [Parinussa, R. M.; de Jeu, R. A. M.] Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Earth Sci, Earth & Climate Cluster, Amsterdam, Netherlands. [Yilmaz, M. T.; Anderson, M. C.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD USA. [Hain, C. R.] Univ Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20742 USA. RP Parinussa, RM (reprint author), Vrije Univ Amsterdam, Fac Earth & Life Sci, Dept Earth Sci, Earth & Climate Cluster, Amsterdam, Netherlands. EM R.M.Parinussa@vu.nl RI Anderson, Martha/C-1720-2015; Hain, Christopher/G-3512-2012 OI Anderson, Martha/0000-0003-0748-5525; Hain, Christopher/0000-0002-0093-6816 FU European Space Agency Climate Change Initiative [4000104814/11/I-NB]; European Commission, EMBRACE project [282672] FX The development of the long-term SM dataset has been undertaken as part of the European Space Agency Climate Change Initiative (http://www.esa-soilmoisturecci.org/, contract no. 4000104814/11/I-NB). Additionally, this work was funded by the European Commission's 7th Framework Programme, under grant agreement number 282672, EMBRACE project. Finally, the authors would like to thank the International Soil Moisture Network hosted by the Vienna University of Technology and particularly Jose Martinez Fernandez from the Universidad de Salamanca for providing the field data. NR 53 TC 11 Z9 12 U1 2 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD AUG 30 PY 2014 VL 28 IS 18 BP 4865 EP 4876 DI 10.1002/hyp.9975 PG 12 WC Water Resources SC Water Resources GA AN8YV UT WOS:000340893000004 ER PT J AU Almeria, S Serrano-Perez, B Darwich, L Araujo, RN Lopez-Gatius, F Dubey, JP Gasbarre, LC AF Almeria, S. Serrano-Perez, B. Darwich, L. Araujo, R. N. Lopez-Gatius, F. Dubey, J. P. Gasbarre, L. C. TI Maternal and fetal immune response patterns in heifers experimentally infected with Neospora caninum in the second trimester of pregnancy - A descriptive study SO VETERINARY PARASITOLOGY LA English DT Article DE Neospora caninum; Bovine; Sequential analysis; Maternal and fetal immune response ID CYTOKINE GENE-EXPRESSION; BLOOD MONONUCLEAR-CELLS; BOVINE NEOSPOROSIS; VERTICAL TRANSMISSION; DAIRY-CATTLE; GESTATION; DEATH; GAMMA; PATHOGENESIS; PROTECTION AB Fetal and maternal immune responses 3, 6 and 9 weeks post infection (wpi) were investigated in cows experimentally infected with Neospora caninum on day 110 of gestation. Descriptive analysis showed that the fetuses had lower percentages of spleen T cell subpopulations (CD3+, CD4+ and CD8+) at 6 wpi compared to 3 wpi and/org wpi, with the lowest percentages observed in a dead fetus found upon euthanasia at that time. Increased expression of most cytokines over levels recorded at 3 and 9 wpi were found in fetuses that were alive at 6 wpi. Up-regulated Th1, Th2 and Treg expression was also observed at 6 wpi in the spleen and in the lymph nodes draining the placenta of the cows. At the placental level, while most cytokines were down-regulated from 6 wpi, up-regulation of IL-4 expression was observed at 6 wpi in the caruncle. Our results suggest that the immune response at 6 wpi was crucial for fetal survival in this model of bovine neosporosis. (C) 2014 Elsevier B.V. All rights reserved. C1 [Almeria, S.; Darwich, L.] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat & Anat Anim, E-08193 Barcelona, Spain. [Serrano-Perez, B.; Lopez-Gatius, F.] Univ Lleida, Agrotecnio Ctr, Lleida, Spain. [Araujo, R. N.] Univ Fed Minas Gerais, ICB, Belo Horizonte, MG, Brazil. [Dubey, J. P.] Agr Res Serv, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Gasbarre, L. C.] Agr Res Serv, Bovine Funct Genom Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Almeria, S (reprint author), Univ Autonoma Barcelona, Fac Vet, Dept Sanitat & Anat Anim, E-08193 Barcelona, Spain. EM Sonia.Almeria@uab.cat RI Serrano-Perez, Beatriz/A-5557-2012; Almeria, Sonia/N-4663-2014; Darwich, Laila/F-5330-2016 OI Serrano-Perez, Beatriz/0000-0002-8772-6335; Almeria, Sonia/0000-0002-0558-5488; Darwich, Laila/0000-0001-9027-9452 FU Beltsville Agriculture Research Service (USA); Spanish MINECO grant [AGL2012-39830-C02-01/02] FX The authors would like to thank the staff of the Beltsville Agriculture Research Center for their valuable help with this experiment and to Ana Burton for reviewing the manuscript's English. This study was partially supported by Beltsville Agriculture Research Service (USA) and by the Spanish MINECO grant (AGL2012-39830-C02-01/02). NR 26 TC 5 Z9 5 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD AUG 29 PY 2014 VL 204 IS 3-4 BP 146 EP 152 DI 10.1016/j.vetpar.2014.05.006 PG 7 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AO6OZ UT WOS:000341472700009 PM 24880649 ER PT J AU Labbe, MA King, DI AF Labbe, Michelle A. King, David I. TI The Effect of Local and Landscape-Level Characteristics on the Abundance of Forest Birds in Early-Successional Habitats during the Post-Fledging Season in Western Massachusetts SO PLOS ONE LA English DT Article ID OVENBIRDS SEIURUS-AUROCAPILLA; JUVENILE SWAINSONS THRUSHES; NEOTROPICAL MIGRANT BIRDS; FLEDGLING OVENBIRDS; BREEDING BIRDS; REGENERATING CLEARCUTS; REPRODUCTIVE SUCCESS; COMMUNITY STRUCTURE; NORTHERN VIRGINIA; MANAGED LANDSCAPE AB Many species of mature forest-nesting birds ("forest birds'') undergo a pronounced shift in habitat use during the post-fledging period and move from their forest nesting sites into areas of early-successional vegetation. Mortality is high during this period, thus understanding the resource requirements of post-fledging birds has implications for conservation. Efforts to identify predictors of abundance of forest birds in patches of early-successional habitats have so far been equivocal, yet these previous studies have primarily focused on contiguously forested landscapes and the potential for landscape-scale influences in more fragmented and modified landscapes is largely unknown. Landscape composition can have a strong influence on the abundance and productivity of forest birds during the nesting period, and could therefore affect the number of forest birds in the landscape available to colonize early-successional habitats during the post-fledging period. Therefore, the inclusion of landscape characteristics should increase the explanatory power of models of forest bird abundance in early-successional habitat patches during the post-fledging period. We examined forest bird abundance and body condition in relation to landscape and habitat characteristics of 15 early-successional sites during the post-fledging season in Massachusetts. The abundance of forest birds was influenced by within-patch habitat characteristics, however the explanatory power of these models was significantly increased by the inclusion of landscape fragmentation and the abundance of forest birds in adjacent forest during the nesting period for some species and age groups. Our findings show that including factors beyond the patch scale can explain additional variation in the abundance of forest birds in early-successional habitats during the post-fledging period. We conclude that landscape composition should be considered when siting early-successional habitat to maximize its benefit to forest birds during the post-fledging period, and should also be included in future investigations of post-fledging habitat use by forest birds. C1 [Labbe, Michelle A.; King, David I.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [King, David I.] Univ Massachusetts, US Forest Serv, No Res Stn, Amherst, MA 01003 USA. RP Labbe, MA (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. EM labbemichelle@gmail.com FU Massachusetts Division of Fisheries and Wildlife FX Funding for the project was provided by Massachusetts Division of Fisheries and Wildlife. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 76 TC 0 Z9 0 U1 5 U2 27 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 29 PY 2014 VL 9 IS 8 AR e106398 DI 10.1371/journal.pone.0106398 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO2EE UT WOS:000341127500156 PM 25170610 ER PT J AU Lam, HN Chakravarthy, S Wei, HL BuiNguyen, H Stodghill, PV Collmer, A Swingle, BM Cartinhour, SW AF Lam, Hanh N. Chakravarthy, Suma Wei, Hai-Lei HoangChuong BuiNguyen Stodghill, Paul V. Collmer, Alan Swingle, Bryan M. Cartinhour, Samuel W. TI Global Analysis of the HrpL Regulon in the Plant Pathogen Pseudomonas syringae pv. tomato DC3000 Reveals New Regulon Members with Diverse Functions SO PLOS ONE LA English DT Article ID SECRETION SYSTEM EFFECTORS; ALTERNATE SIGMA-FACTOR; III PROTEIN SECRETION; ESCHERICHIA-COLI; GENOMEWIDE IDENTIFICATION; CULTIVAR SPECIFICITY; AVIRULENCE GENE; PROMOTER; SEQUENCE; TRANSLOCATION AB The type III secretion system (T3SS) is required for virulence in the gram-negative plant pathogen Pseudomonas syringae pv. tomato DC3000. The alternative sigma factor HrpL directly regulates expression of T3SS genes via a promoter sequence, often designated as the "hrp promoter." Although the HrpL regulon has been extensively investigated in DC3000, it is not known whether additional regulon members remain to be found. To systematically search for HrpL-regulated genes, we used chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-Seq) and bulk mRNA sequencing (RNA-Seq) to identify HrpL-binding sites and likely hrp promoters. The analysis recovered 73 sites of interest, including 20 sites that represent new hrp promoters. The new promoters lie upstream of a diverse set of genes encoding potential regulators, enzymes and hypothetical proteins. PSPTO_5633 is the only new HrpL regulon member that is potentially an effector and is now designated HopBM1. Deletions in several other new regulon members, including PSPTO_5633, PSPTO_0371, PSPTO_2130, PSPTO_2691, PSPTO_2696, PSPTO_3331, and PSPTO_5240, in either DC3000 or Delta hopQ1-1 backgrounds, do not affect the hypersensitive response or in planta growth of the resulting strains. Many new HrpL regulon members appear to be unrelated to the T3SS, and orthologs for some of these can be identified in numerous nonpathogenic bacteria. With the identification of 20 new hrp promoters, the list of HrpL regulon members is approaching saturation and most likely includes all DC3000 effectors. C1 [Lam, Hanh N.; Chakravarthy, Suma; Wei, Hai-Lei; HoangChuong BuiNguyen; Stodghill, Paul V.; Collmer, Alan; Swingle, Bryan M.; Cartinhour, Samuel W.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14850 USA. [Stodghill, Paul V.; Swingle, Bryan M.; Cartinhour, Samuel W.] USDA ARS, Ithaca, NY 14853 USA. RP Cartinhour, SW (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14850 USA. EM sam.cartinhour@ars.usda.gov FU USDA-ARS (U.S. Department of Agriculture-Agricultural Research Service) CRIS; NSF [IOS-1025642]; Vietnam Education Foundation (VEF) FX This work was supported by USDA-ARS (U.S. Department of Agriculture-Agricultural Research Service) CRIS, by NSF funding IOS-1025642, and by a graduate research fellowship awarded to Cornell student H. Lam by the Vietnam Education Foundation (VEF). Three authors (P. Stodghill, B. Swingle and S. Cartinhour) are employees of the USDA-ARS. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 80 TC 6 Z9 6 U1 2 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 29 PY 2014 VL 9 IS 8 AR e106115 DI 10.1371/journal.pone.0106115 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO2EE UT WOS:000341127500107 PM 25170934 ER PT J AU Strong, AL Ohlstein, JF Jiang, Q Zhang, Q Zheng, SL Boue, SM Elliott, S Gimble, JM Burow, ME Wang, GD Bunnell, BA AF Strong, Amy L. Ohlstein, Jason F. Jiang, Quan Zhang, Qiang Zheng, Shilong Boue, Stephen M. Elliott, Steven Gimble, Jeffrey M. Burow, Matthew E. Wang, Guangdi Bunnell, Bruce A. TI Novel daidzein analogs enhance osteogenic activity of bone marrow-derived mesenchymal stem cells and adipose-derived stromal/stem cells through estrogen receptor dependent and independent mechanisms SO STEM CELL RESEARCH & THERAPY LA English DT Article ID BREAST-CANCER; POSTMENOPAUSAL WOMEN; OSTEOPOROTIC FRACTURE; REPLACEMENT THERAPY; GENISTEIN AGLYCONE; HIGH-FAT; TISSUE; DIFFERENTIATION; MICE; RISK AB Introduction: Osteoporosis is a disease characterized by low bone mineral density (BMD) and increased risk of fractures. Studies have demonstrated the use of phytoestrogens, or plant-derived estrogens, such as genistein and daidzein, to effectively increase osteogenic activity of bone marrow-derived mesenchymal stem cells (BMSCs). Herein, the effects of daidzein analogs on the osteogenic differentiation efficiency of human BMSC and adipose-derived stromal/stem cells (ASC) were explored. Methods: BMSCs and ASCs underwent osteogenic differentiation in the presence of vehicle, 17 beta-estradiol (E2), phytoestrogens, or daidzein analogs. Cells were stained for alkaline phosphatase (ALP) enzymatic activity, calcium deposition by alizarin red s, and phosphate mineralization by silver nitrate. Gene expression analysis was conducted on cells treated with daidzein analogs. Results: Cells treated with E2, daidzein, or genistein increased calcium deposition by 1.6-, 1.5-, and 1.4-fold, respectively, relative to vehicle-treated BMSCs and 1.6-, 1.7-, and 1.4-fold relative to vehicle-treated ASCs, respectively. BMSCs treated with daidzein analog 2c, 2g, and 2l demonstrated a 1.6-, 1.6-, and 1.9-fold increase in calcium deposition relative to vehicle-treated BMSCs, respectively, while ASCs treated with daidzein analog 2c, 2g, or 2l demonstrated a 1.7-, 2.0-, and 2.2-fold increase in calcium deposition relative to vehicle-treated ASCs, respectively. Additional analysis with BMSCs and ASCs was conducted in the more efficient compounds: 2g and 2l. ALP activity and phosphate mineralization was increased in 2g- and 2l-treated cells. The analysis of lineage specific gene expression demonstrated increased expression of key osteogenic genes (RUNX2, c-FOS, SPARC, DLX5, SPP1, COL1A1, IGF1, SOST, and DMP1) and earlier induction of these lineage specific genes, following treatment with 2g or 2l, relative to vehicle-treated cells. Estrogen receptor (ER) inhibitor studies demonstrated that ER antagonist fulvestrant inhibited the osteogenic differentiation of 2g in BMSCs and ASCs, while fulvestrant only attenuated the effects of 2l, suggesting that 2l acts by both ER dependent and independent pathways. Conclusions: These studies provide support for exploring the therapeutic efficacy of daidzein derivatives for the treatment of osteoporosis. Furthermore, the patterns of gene induction differed following treatment with each daidzein analog, suggesting that these daidzein analogs activate distinct ER and non-ER pathways to induce differentiation in BMSCs and ASCs. C1 [Strong, Amy L.; Ohlstein, Jason F.; Gimble, Jeffrey M.; Bunnell, Bruce A.] Tulane Univ, Sch Med, Ctr Stem Cell Res & Regenerat Med, New Orleans, LA 70112 USA. [Jiang, Quan; Zhang, Qiang; Zheng, Shilong; Wang, Guangdi] Xavier Univ Louisiana, Dept Chem, New Orleans, LA 70125 USA. [Jiang, Quan; Zhang, Qiang; Zheng, Shilong; Wang, Guangdi] Xavier Univ Louisiana, RCMI Canc Res Program, New Orleans, LA 70125 USA. [Boue, Stephen M.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Elliott, Steven; Burow, Matthew E.] Tulane Univ, Sch Med, Dept Med, New Orleans, LA 70112 USA. RP Bunnell, BA (reprint author), Tulane Univ, Sch Med, Ctr Stem Cell Res & Regenerat Med, 1430 Tulane Ave,SL-99, New Orleans, LA 70112 USA. EM bbunell@tulane.edu FU National Center for Research Resources RCMI program [1G12RR026260-01]; US Department of Agriculture [58-6435-7-019]; Louisiana Cancer Research Consortium; National Institute of General Medical Sciences of the National Institutes of Health [1U54GM104940]; Tulane University pilot grant FX This study was supported by the National Center for Research Resources RCMI program through Grant 1G12RR026260-01, US Department of Agriculture Grant 58-6435-7-019, the Louisiana Cancer Research Consortium, National Institute of General Medical Sciences of the National Institutes of Health 1U54GM104940 (Louisiana Clinical and Translational Science Center), and funds from Tulane University pilot grant. NR 58 TC 6 Z9 6 U1 1 U2 11 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1757-6512 J9 STEM CELL RES THER JI Stem Cell Res. Ther. PD AUG 28 PY 2014 VL 5 AR 105 DI 10.1186/scrt493 PG 17 WC Cell Biology; Medicine, Research & Experimental SC Cell Biology; Research & Experimental Medicine GA AS9NC UT WOS:000344568300001 PM 25168698 ER PT J AU Zhang, L Jia, BG Tan, XF Thammina, CS Long, HX Liu, M Wen, SN Song, XL Cao, HP AF Zhang, Lin Jia, Baoguang Tan, Xiaofeng Thammina, Chandra S. Long, Hongxu Liu, Min Wen, Shanna Song, Xianliang Cao, Heping TI Fatty Acid Profile and Unigene-Derived Simple Sequence Repeat Markers in Tung Tree (Vernicia fordii) SO PLOS ONE LA English DT Article ID GENETIC-LINKAGE MAP; SSR-MARKERS; RNA-SEQ; MICROSATELLITES; OIL; EXPRESSION; EVOLUTION; GENOME; DIVERSITY; CUCURBITA AB Tung tree (Vernicia fordii) provides the sole source of tung oil widely used in industry. Lack of fatty acid composition and molecular markers hinders biochemical, genetic and breeding research. The objectives of this study were to determine fatty acid profiles and develop unigene-derived simple sequence repeat (SSR) markers in tung tree. Fatty acid profiles of 41 accessions showed that the ratio of alpha-eleostearic acid was increasing continuously with a parallel trend to the amount of tung oil accumulation while the ratios of other fatty acids were decreasing in different stages of the seeds and that alpha-eleostearic acid (18: 3) consisted of 77% of the total fatty acids in tung oil. Transcriptome sequencing identified 81,805 unigenes from tung cDNA library constructed using seed mRNA and discovered 6,366 SSRs in 5,404 unigenes. The di- and tri-nucleotide microsatellites accounted for 92% of the SSRs with AG/CT and AAG/CTT being the most abundant SSR motifs. Fifteen polymorphic genic-SSR markers were developed from 98 unigene loci tested in 41 cultivated tung accessions by agarose gel and capillary electrophoresis. Genbank database search identified 10 of them putatively coding for functional proteins. Quantitative PCR demonstrated that all 15 polymorphic SSR-associated unigenes were expressed in tung seeds and some of them were highly correlated with oil composition in the seeds. Dendrogram revealed that most of the 41 accessions were clustered according to the geographic region. These new polymorphic genic-SSR markers will facilitate future studies on genetic diversity, molecular fingerprinting, comparative genomics and genetic mapping in tung tree. The lipid profiles in the seeds of 41 tung accessions will be valuable for biochemical and breeding studies. C1 [Zhang, Lin; Jia, Baoguang; Tan, Xiaofeng; Long, Hongxu; Liu, Min; Wen, Shanna] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China. [Zhang, Lin; Thammina, Chandra S.] Univ Connecticut, Dept Plant Sci & Landscape Architecture, Storrs, CT USA. [Song, Xianliang; Cao, Heping] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. RP Tan, XF (reprint author), Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China. EM tanxiaofengcn@126.com; Heping.Cao@ars.usda.gov FU "948 Project'' of China State Forestry Administration [2012-4-42]; National Forestry Public Welfare Industry Research Project of China [201204403]; Key Project of Chinese Ministry of Education [212126]; USDA-ARS Quality and Utilization of Agricultural Products Research Program 306 through CRIS [6435-41000-102-00D, 6435-41000-102-10N] FX This work was supported by the "948 Project'' of China State Forestry Administration(2012-4-42), National Forestry Public Welfare Industry Research Project of China(201204403), the Key Project of Chinese Ministry of Education (212126), and USDA-ARS Quality and Utilization of Agricultural Products Research Program 306 through CRIS 6435-41000-102-00D and 6435-41000-102-10N. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 5 Z9 6 U1 2 U2 23 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 28 PY 2014 VL 9 IS 8 AR e105298 DI 10.1371/journal.pone.0105298 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO4JP UT WOS:000341303700021 PM 25167054 ER PT J AU Lippolis, JD Brunelle, BW Reinhardt, TA Sacco, RE Nonnecke, BJ Dogan, B Simpson, K Schukken, YH AF Lippolis, John D. Brunelle, Brian W. Reinhardt, Timothy A. Sacco, Randy E. Nonnecke, Brian J. Dogan, Belgin Simpson, Kenneth Schukken, Ynte H. TI Proteomic analysis reveals protein expression differences in Escherichia coli strains associated with persistent versus transient mastitis SO JOURNAL OF PROTEOMICS LA English DT Article DE Mastitis; Bacterial motility; Swimming; Swarming; Bacterial proteome ID LARGE GENE LISTS; SWARMING MOTILITY; STATISTICAL-MODEL; CLINICAL MASTITIS; MAMMARY-GLAND; BACTERIAL; MILK; CHEMOTAXIS; SYSTEM AB Escherichia coli is a leading cause of bacterial mastitis in dairy cattle. Typically this infection is transient in nature, causing an infection that lasts 2-3 days. However, in a minority of cases, E. coli has been shown to cause a persistent infection. The mechanisms that allow for a persistent E. coli infection are not fully understood. The goal of this work was to determine protein expression differences between E. coli strains isolated from dairy cattle with transient and persistent mastitis infections. Three persistent and three transient mastitis-derived strains of E. coli were compared using iTRAQ in a shotgun proteomics experiment. Expression data for 1127 proteins were determined. Of these, 28 proteins were associated with expression changes correlated with a difference in disease phenotype. Of particular interest were proteins that have been shown to be essential for bacterial swimming and swarming. Bacterial swimming and swarming assays showed that the strains from the persistent mastitis cases were significantly better in these motility assays than the strains from the transient cases. This work identifies important protein expression differences between E. coli strains that cause a persistent versus a transient infection as well as demonstrates a corresponding difference in the associated bacterial motility phenotypes. Biological significance The significance of this study is that proteins associated with bacterial swimming and swarming are more highly expressed in the E. coli strains that cause persistent mastitis infections. These findings point to swimming and swarming as important mechanisms involved in how a pathogen establishes a persistent infection in the mammary gland. The role of swimming and swarming in clinical mastitis clearly requires further experimentation. Published by Elsevier B.V. C1 [Lippolis, John D.] USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, Ames, IA 50010 USA. USDA ARS, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, Ames, IA 50010 USA. Cornell Univ, Coll Vet Med, Ithaca, NY 14853 USA. RP Lippolis, JD (reprint author), USDA ARS, Natl Anim Dis Ctr, Ruminant Dis & Immunol Res Unit, POB 70, Ames, IA 50010 USA. EM John.Lippolis@ars.usda.gov RI Reinhardt, Timothy/A-7536-2009; OI Reinhardt, Timothy/0000-0001-5552-2509; Schukken, Ynte/0000-0002-8250-4194 NR 27 TC 9 Z9 9 U1 1 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1874-3919 EI 1876-7737 J9 J PROTEOMICS JI J. Proteomics PD AUG 28 PY 2014 VL 108 BP 373 EP 381 DI 10.1016/j.jprot.2014.06.008 PG 9 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA AN1AP UT WOS:000340315400030 PM 24954099 ER PT J AU Fu, Y Liu, LS Zhang, JW AF Fu, Yu Liu, Linshu Zhang, Jinwen TI Manipulating Dispersion and Distribution of Graphene in PLA through Novel Interface Engineering for Improved Conductive Properties SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE graphene; functionalization; dispersion; polylactic acid; nanocomposites; conductivity ID MULTIWALLED CARBON NANOTUBES; POLYMER BLENDS; ELECTRICAL-CONDUCTIVITY; GRAPHITE OXIDE; SELECTIVE LOCALIZATION; PHASE MORPHOLOGY; NANOCOMPOSITES; PERCOLATION; BLACK; COMPOSITES AB This study aimed to enhance the conductive properties of PLA nanocomposite by controlling the dispersion and distribution of graphene within the minor phase of the polymer blend. Functionalized graphene (f-GO) was achieved by reacting graphene oxide (GO) with various silanes under the aid of an ionic liquid. Ethylene/n-butyl acrylate/glycidyl methacrylate terpolymer elastomer (EBA-GMA) was introduced as the minor phase to tailor the interface of matrix/graphene through reactive compatibilization. GO particles were predominantly dispersed in PLA in a self-agglomerating pattern, while f-GO was preferentially located in the introduced rubber phase or at the PLA/EBA-GMA interfaces through the formation of the three-dimensional percolated structures, especially for these functionalized graphene with reactive groups. The selective localization of the f-GO also played a crucial role in stabilizing and improving the phase morphology of the PLA blend through reducing the interfacial tension between two phases. The establishment of the percolated network structures in the ternary system was responsible for the improved AC conductivity and better dielectric properties of the resulting nanocomposites. C1 [Fu, Yu; Zhang, Jinwen] Washington State Univ, Composite Mat & Engn Ctr, Sch Mech & Mat Engn, Pullman, WA 99164 USA. [Liu, Linshu] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Zhang, JW (reprint author), Washington State Univ, Composite Mat & Engn Ctr, Sch Mech & Mat Engn, Pullman, WA 99164 USA. EM jwzhang@wsu.edu NR 42 TC 13 Z9 13 U1 12 U2 143 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD AUG 27 PY 2014 VL 6 IS 16 BP 14069 EP 14075 DI 10.1021/am503283f PG 7 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA AO2CB UT WOS:000341122000091 PM 25014782 ER PT J AU Yu, L Huang, HQ Yu, LL Wang, TTY AF Yu, Lu Huang, Haiqiu Yu, Liangli (Lucy) Wang, Thomas T. Y. TI Utility of Hesperidinase for Food Function Research: Enzymatic Digestion of Botanical Extracts Alters Cellular Antioxidant Capacities and Anti-inflammatory Properties SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE hesperidinase; enzymatic digestion; antioxidant; anti-inflammation; xenobiotic metabolism ID PHENOLIC-COMPOUNDS; OXIDATIVE STRESS; FLAVONOIDS; METABOLISM; CANCER; INFLAMMATION; EXPRESSION; CHEMISTRY; CELLS; BIOAVAILABILITY AB Food-derived phytochemicals, many known for their health beneficial effects, often exist in conjugated forms containing sugar moieties such as glucose or rhamnose in foods. The uptake of these compounds requires colonic bacterial cleavage of sugar moieties. However, most studies involved in screening extracts for biological activities do not take this process into account. This study seeks to determine the utility of commercially available hesperidinase to mimic colonic digestion and to test the effects of this treatment on the biological properties of extracts. Using hesperidinase resulted in efficient hydrolysis of Engelhardia roxburghiana Wall. extract containing rhamnose conjugates. Enzymatic digestion enhanced the extract's cellular antioxidant ability by 2-fold in HepG2/C3A and the anti-inflammatory effect on lipopolysaccharide-induced interleukin (IL)-1 beta and IL-6 expression in mouse macrophage J774A.1 and human monocyte THP-1 cells. Enzymatic digestion also efficiently processed extracts with mixed rhamnose and glucose conjugates and altered their biological activities. Results of the present study supported the importance of considering enzymatic digestion during the biological activity studies of botanicals. C1 [Yu, Lu; Huang, Haiqiu; Yu, Liangli (Lucy)] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Wang, Thomas T. Y.] ARS, Diet Genom & Immunol Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Yu, LL (reprint author), Univ Maryland, Dept Nutr & Food Sci, 0112 Skinner Bldg, College Pk, MD 20742 USA. EM lyu5@umd.edu; Tom.Wang@ars.usda.gov RI Huang, Haiqiu/J-8856-2012 FU USDA [1235-51530-053-00D] FX This research was supported by USDA appropriate fund Project 1235-51530-053-00D (T.T.Y.W.). NR 35 TC 6 Z9 6 U1 1 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 27 PY 2014 VL 62 IS 34 BP 8640 EP 8647 DI 10.1021/jf501963a PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AO0IK UT WOS:000340992100013 PM 25093531 ER PT J AU Ostertag, R Inman-Narahari, F Cordell, S Giardina, CP Sack, L AF Ostertag, Rebecca Inman-Narahari, Faith Cordell, Susan Giardina, Christian P. Sack, Lawren TI Forest Structure in Low-Diversity Tropical Forests: A Study of Hawaiian Wet and Dry Forests SO PLOS ONE LA English DT Article ID ABOVEGROUND LIVE BIOMASS; RAIN-FOREST; SPECIES RICHNESS; LOWLAND FORESTS; CLOUD FOREST; TREE FERNS; PLANT COMMUNITY; BASAL AREA; FERAL PIGS; DYNAMICS AB The potential influence of diversity on ecosystem structure and function remains a topic of significant debate, especially for tropical forests where diversity can range widely. We used Center for Tropical Forest Science (CTFS) methodology to establish forest dynamics plots in montane wet forest and lowland dry forest on Hawai'i Island. We compared the species diversity, tree density, basal area, biomass, and size class distributions between the two forest types. We then examined these variables across tropical forests within the CTFS network. Consistent with other island forests, the Hawai'i forests were characterized by low species richness and very high relative dominance. The two Hawai'i forests were floristically distinct, yet similar in species richness (15 vs. 21 species) and stem density (3078 vs. 3486/ha). While these forests were selected for their low invasive species cover relative to surrounding forests, both forests averaged 5->50% invasive species cover; ongoing removal will be necessary to reduce or prevent competitive impacts, especially from woody species. The montane wet forest had much larger trees, resulting in eightfold higher basal area and above-ground biomass. Across the CTFS network, the Hawaiian montane wet forest was similar to other tropical forests with respect to diameter distributions, density, and aboveground biomass, while the Hawai` i lowland dry forest was similar in density to tropical forests with much higher diversity. These findings suggest that forest structural variables can be similar across tropical forests independently of species richness. The inclusion of low-diversity Pacific Island forests in the CTFS network provides an similar to 80-fold range in species richness (15-1182 species), six-fold variation in mean annual rainfall (835-5272 mm yr(-1)) and 1.8-fold variation in mean annual temperature (16.0-28.4 degrees C). Thus, the Hawaiian forest plots expand the global forest plot network to enable testing of ecological theory for links among species diversity, environmental variation and ecosystem function. C1 [Ostertag, Rebecca] Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. [Inman-Narahari, Faith] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Cordell, Susan; Giardina, Christian P.] US Forest Serv, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, USDA, Hilo, HI USA. [Sack, Lawren] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA USA. RP Ostertag, R (reprint author), Univ Hawaii, Dept Biol, Hilo, HI 96720 USA. EM ostertag@hawaii.edu RI Giardina, Christian/C-3120-2011; Sack, Lawren/A-5492-2008 OI Giardina, Christian/0000-0002-3431-5073; Sack, Lawren/0000-0002-7009-7202 FU National Science Foundation's EPSCoR [0554657, 0903833]; Smithsonian Tropical Research Institute Center for Tropical Forest Science; University of California, Los Angeles FX The major funding for this research came from National Science Foundation's EPSCoR Grants No. 0554657 and No. 0903833 to the University of Hawai 'i. Major in-kind support was provided by the Pacific Southwest Research Station of the United States Forest Service. Logistical or financial support that was supplemental was provided for by the Smithsonian Tropical Research Institute Center for Tropical Forest Science, and the University of California, Los Angeles. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 102 TC 7 Z9 7 U1 2 U2 40 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 27 PY 2014 VL 9 IS 8 AR e103268 DI 10.1371/journal.pone.0103268 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN8UR UT WOS:000340880900006 PM 25162731 ER PT J AU Mao, YQ Van Auken, K Li, DH Arighi, CN McQuilton, P Hayman, GT Tweedie, S Schaeffer, ML Laulederkind, SJF Wang, SJ Gobeill, J Ruch, P Luu, AT Kim, JJ Chiang, JH Chen, YD Yang, CJ Liu, HF Zhu, DQ Li, YP Yu, H Emadzadeh, E Gonzalez, G Chen, JM Dai, HJ Lu, ZY AF Mao, Yuqing Van Auken, Kimberly Li, Donghui Arighi, Cecilia N. McQuilton, Peter Hayman, G. Thomas Tweedie, Susan Schaeffer, Mary L. Laulederkind, Stanley J. F. Wang, Shur-Jen Gobeill, Julien Ruch, Patrick Anh Tuan Luu Kim, Jung-jae Chiang, Jung-Hsien Chen, Yu-De Yang, Chia-Jung Liu, Hongfang Zhu, Dongqing Li, Yanpeng Yu, Hong Emadzadeh, Ehsan Gonzalez, Graciela Chen, Jian-Ming Dai, Hong-Jie Lu, Zhiyong TI Overview of the gene ontology task at BioCreative IV SO DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION LA English DT Article ID ANNOTATION; EXTRACTION; TOOL AB Gene Ontology (GO) annotation is a common task among model organism databases (MODs) for capturing gene function data from journal articles. It is a time-consuming and labor-intensive task, and is thus often considered as one of the bottlenecks in literature curation. There is a growing need for semiautomated or fully automated GO curation techniques that will help database curators to rapidly and accurately identify gene function information in full-length articles. Despite multiple attempts in the past, few studies have proven to be useful with regard to assisting real-world GO curation. The shortage of sentence-level training data and opportunities for interaction between text-mining developers and GO curators has limited the advances in algorithm development and corresponding use in practical circumstances. To this end, we organized a text-mining challenge task for literature-based GO annotation in BioCreative IV. More specifically, we developed two subtasks: (i) to automatically locate text passages that contain GO-relevant information (a text retrieval task) and (ii) to automatically identify relevant GO terms for the genes in a given article (a concept-recognition task). With the support from five MODs, we provided teams with >4000 unique text passages that served as the basis for each GO annotation in our task data. Such evidence text information has long been recognized as critical for text-mining algorithm development but was never made available because of the high cost of curation. In total, seven teams participated in the challenge task. From the team results, we conclude that the state of the art in automatically mining GO terms from literature has improved over the past decade while much progress is still needed for computer-assisted GO curation. Future work should focus on addressing remaining technical challenges for improved performance of automatic GO concept recognition and incorporating practical benefits of text-mining tools into real-world GO annotation. C1 [Mao, Yuqing; Lu, Zhiyong] Natl Lib Med, Natl Ctr Biotechnol Informat NCBI, Bethesda, MD 20817 USA. [Van Auken, Kimberly] CALTECH, Div Biol, WormBase, Pasadena, CA 91125 USA. [Li, Donghui] Carnegie Inst Sci, Dept Plant Biol, TAIR, Stanford, CA 94305 USA. [Arighi, Cecilia N.] Univ Delaware, Ctr Bioinformat & Computat Biol, Newark, DE 19711 USA. [McQuilton, Peter; Tweedie, Susan] Univ Cambridge, Dept Genet, FlyBase, Cambridge CB2 3EH, England. [Hayman, G. Thomas; Laulederkind, Stanley J. F.; Wang, Shur-Jen] Med Coll Wisconsin, Rat Genome Database Human & Mol Genet Ctr, Milwaukee, WI 53226 USA. [Schaeffer, Mary L.] ARS, USDA, Plant Genet Res Unit, Columbia, MO 65211 USA. [Schaeffer, Mary L.] Univ Missouri, Div Plant Sci, Dept Agron, Columbia, MO 65211 USA. [Gobeill, Julien; Ruch, Patrick] HEG, HES SO, CH-1227 Carouge, Switzerland. [Gobeill, Julien; Ruch, Patrick] Swiss Inst Bioinformat, SIBtex, CH-1211 Geneva 4, Switzerland. [Anh Tuan Luu; Kim, Jung-jae] Nanyang Technol Univ, Sch Comp Engn, Singapore 639798, Singapore. [Chiang, Jung-Hsien; Chen, Yu-De; Yang, Chia-Jung] Natl Cheng Kung Univ, Dept Comp Sci & Informat Engn, Tainan 701, Taiwan. [Yang, Chia-Jung] Mackay Mem Hosp, Dept Radiol, Taitung Branch, Taitung, Taiwan. [Liu, Hongfang; Zhu, Dongqing] Mayo Clin, Dept Hlth Sci Res, Rochester, MN 55905 USA. [Zhu, Dongqing] Univ Delaware, Dept Comp Sci, Newark, DE 19716 USA. [Li, Yanpeng; Yu, Hong] Univ Massachusetts, Dept Quantitat Hlth Sci, Sch Med, Worcester, MA 01655 USA. [Emadzadeh, Ehsan; Gonzalez, Graciela] Arizona State Univ, Dept Biomed Informat, Scottsdale, AZ 85259 USA. [Chen, Jian-Ming] Acad Sinica, Inst Informat Sci, Taipei 115, Taiwan. [Dai, Hong-Jie] Taipei Med Univ, Grad Inst BioMed Informat, Coll Med Sci & Technol, Taipei 110, Taiwan. RP Lu, ZY (reprint author), Natl Lib Med, Natl Ctr Biotechnol Informat NCBI, 8600 Rockville Pike, Bethesda, MD 20817 USA. EM Zhiyong.Lu@nih.gov RI Kim, Jung-jae/A-3738-2011; OI McQuilton, Peter/0000-0003-2687-1982; Tweedie, Susan/0000-0003-1818-8243; Gonzalez Hernandez, Graciela/0000-0002-6416-9556; Arighi, Cecilia/0000-0002-0803-4817 FU NIH Intramural Research Program, National Library of Medicine; National Human Genome Research Institute [U41-HG002223]; Gene Ontology Consortium by National Human Genome Research Institute (NHGRI) [U41-HG002273]; NHGRI/NIH [U41-HG000739]; UK Medical Research Council [G1000968]; NIH [R01LM009959A1]; SNF [153437]; European Union [257528]; [NSF/DBI-0850319]; [NSF/ABI-0845523] FX This research is supported by NIH Intramural Research Program, National Library of Medicine (Y.M. and Z.L.). The BioCreative IV Workshop is funded by NSF/DBI-0850319. WormBase is funded by National Human Genome Research Institute [U41-HG002223] and the Gene Ontology Consortium by National Human Genome Research Institute (NHGRI) [U41-HG002273]. FlyBase is funded by an NHGRI/NIH grant [U41-HG000739] and the UK Medical Research Council [G1000968]. Team 238 is funded by NSF/ABI-0845523 (H. L. and D.Z.), NIH R01LM009959A1 (H. L. and D.Z.). The SIBtex (Swiss Institute of Bioinformatics) team has been partially supported by the SNF (neXtpresso #153437) and the European Union (Khresmoi #257528). NR 67 TC 12 Z9 12 U1 0 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1758-0463 J9 DATABASE-OXFORD JI Database PD AUG 25 PY 2014 AR bau086 DI 10.1093/database/bau086 PG 14 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA AO4KY UT WOS:000341308000001 ER PT J AU Lauter, ANM Peiffer, GA Yin, TF Whitham, SA Cook, D Shoemaker, RC Graham, MA AF Lauter, Adrienne N. Moran Peiffer, Gregory A. Yin, Tengfei Whitham, Steven A. Cook, Dianne Shoemaker, Randy C. Graham, Michelle A. TI Identification of candidate genes involved in early iron deficiency chlorosis signaling in soybean (Glycine max) roots and leaves SO BMC GENOMICS LA English DT Article DE Iron deficiency chlorosis; Soybean; Glycine max; RNA-Seq ID DIFFERENTIAL EXPRESSION ANALYSIS; BINDING TRANSCRIPTION FACTORS; DOMAIN-CONTAINING PROTEIN; COLD-INDUCIBLE GENE; ARABIDOPSIS-THALIANA; ABSCISIC-ACID; NITRIC-OXIDE; OSMOTIC-STRESS; MEMBRANE TRANSPORTER; MICROARRAY ANALYSIS AB Background: Iron is an essential micronutrient for all living things, required in plants for photosynthesis, respiration and metabolism. A lack of bioavailable iron in soil leads to iron deficiency chlorosis (IDC), causing a reduction in photosynthesis and interveinal yellowing of leaves. Soybeans (Glycine max (L.) Merr.) grown in high pH soils often suffer from IDC, resulting in substantial yield losses. Iron efficient soybean cultivars maintain photosynthesis and have higher yields under IDC-promoting conditions than inefficient cultivars. Results: To capture signaling between roots and leaves and identify genes acting early in the iron efficient cultivar Clark, we conducted a RNA-Seq study at one and six hours after replacing iron sufficient hydroponic media (100 mu M iron(III) nitrate nonahydrate) with iron deficient media (50 mu M iron(III) nitrate nonahydrate). At one hour of iron stress, few genes were differentially expressed in leaves but many were already changing expression in roots. By six hours, more genes were differentially expressed in the leaves, and a massive shift was observed in the direction of gene expression in both roots and leaves. Further, there was little overlap in differentially expressed genes identified in each tissue and time point. Conclusions: Genes involved in hormone signaling, regulation of DNA replication and iron uptake utilization are key aspects of the early iron-efficiency response. We observed dynamic gene expression differences between roots and leaves, suggesting the involvement of many transcription factors in eliciting rapid changes in gene expression. In roots, genes involved iron uptake and development of Casparian strips were induced one hour after iron stress. In leaves, genes involved in DNA replication and sugar signaling responded to iron deficiency. The differentially expressed genes (DEGs) and signaling components identified here represent new targets for soybean improvement. C1 [Lauter, Adrienne N. Moran; Peiffer, Gregory A.; Shoemaker, Randy C.; Graham, Michelle A.] USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Yin, Tengfei; Cook, Dianne] Iowa State Univ, Dept Stat, Ames, IA 50011 USA. [Whitham, Steven A.] Iowa State Univ, Dept Plant Pathol & Microbiol, Ames, IA 50011 USA. [Shoemaker, Randy C.; Graham, Michelle A.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Graham, MA (reprint author), USDA ARS, Corn Insects & Crop Genet Res Unit, 1565 Agron Hall, Ames, IA 50011 USA. EM michelle.graham@ars.usda.gov FU North Central Soybean Research Program; Iowa Soybean Association; USDA-ARS CRIS [3625-21220-005-00D] FX We thank Dr. Jamie O'Rourke and Lori Lincoln for review of the manuscript. This work is supported by the North Central Soybean Research Program, the Iowa Soybean Association and USDA-ARS CRIS Project 3625-21220-005-00D. NR 143 TC 11 Z9 11 U1 4 U2 39 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 22 PY 2014 VL 15 AR 702 DI 10.1186/1471-2164-15-702 PG 25 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AO7JM UT WOS:000341529300001 ER PT J AU Dempsey, SJ Gese, EM Kluever, BM AF Dempsey, Steven J. Gese, Eric M. Kluever, Bryan M. TI Finding a Fox: An Evaluation of Survey Methods to Estimate Abundance of a Small Desert Carnivore SO PLOS ONE LA English DT Article ID JOAQUIN KIT FOXES; GREAT-BASIN DESERT; VULPES-MACROTIS-MUTICA; SCAT DETECTION DOGS; HOME-RANGE SIZE; SWIFT FOXES; OCCUPANCY RATES; HABITAT USE; POPULATIONS; COYOTES AB The status of many carnivore species is a growing concern for wildlife agencies, conservation organizations, and the general public. Historically, kit foxes (Vulpes macrotis) were classified as abundant and distributed in the desert and semi-arid regions of southwestern North America, but is now considered rare throughout its range. Survey methods have been evaluated for kit foxes, but often in populations where abundance is high and there is little consensus on which technique is best to monitor abundance. We conducted a 2-year study to evaluate four survey methods (scat deposition surveys, scent station surveys, spotlight survey, and trapping) for detecting kit foxes and measuring fox abundance. We determined the probability of detection for each method, and examined the correlation between the relative abundance as estimated by each survey method and the known minimum kit fox abundance as determined by radio-collared animals. All surveys were conducted on 15 5-km transects during the 3 biological seasons of the kit fox. Scat deposition surveys had both the highest detection probabilities (p = 0.88) and were most closely related to minimum known fox abundance (r(2) = 0.50, P = 0.001). The next best method for kit fox detection was the scent station survey (p = 0.73), which had the second highest correlation to fox abundance (r(2) = 0.46, P<0.001). For detecting kit foxes in a low density population we suggest using scat deposition transects during the breeding season. Scat deposition surveys have low costs, resilience to weather, low labor requirements, and pose no risk to the study animals. The breeding season was ideal for monitoring kit fox population size, as detections consisted of the resident population and had the highest detection probabilities. Using appropriate monitoring techniques will be critical for future conservation actions for this rare desert carnivore. C1 [Dempsey, Steven J.; Kluever, Bryan M.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Gese, Eric M.] Utah State Univ, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv,Wildlife Res Ctr,Dept Wildland Reso, Logan, UT 84322 USA. RP Gese, EM (reprint author), Utah State Univ, USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv,Wildlife Res Ctr,Dept Wildland Reso, Logan, UT 84322 USA. EM eric.gese@usu.edu RI Gese, Eric/B-4578-2011 FU Department of Defense, United States Army Dugway Proving Ground, Environmental Programs, Dugway, Utah; United States Department of Agriculture, Wildlife Services, National Wildlife Research Center, Utah State University, Logan, Utah; Quinney College of Natural Resources, Utah State University, Logan, Utah; Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources, Salt Lake City, Utah FX Funding and logistical support were provided by the Department of Defense, United States Army Dugway Proving Ground, Environmental Programs, Dugway, Utah, and the United States Department of Agriculture, Wildlife Services, National Wildlife Research Center, Utah State University, Logan, Utah. Additional funding was provided by the Quinney College of Natural Resources, Utah State University, Logan, Utah, and the Endangered Species Mitigation Fund of the Utah Department of Natural Resources, Division of Wildlife Resources, Salt Lake City, Utah. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 52 TC 6 Z9 6 U1 10 U2 57 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 22 PY 2014 VL 9 IS 8 AR e105873 DI 10.1371/journal.pone.0105873 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO3LL UT WOS:000341230600092 PM 25148102 ER PT J AU Somleva, MN Xu, CA Ryan, KP Thilmony, R Peoples, O Snell, KD Thomson, J AF Somleva, Maria N. Xu, Chang Ai Ryan, Kieran P. Thilmony, Roger Peoples, Oliver Snell, Kristi D. Thomson, James TI Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase SO BMC BIOTECHNOLOGY LA English DT Article DE Autoexcision; Bxb1 site-specific recombinase; Developmentally programmed transgene excision; Pollen-mediated gene flow; Panicum virgatum L.; Switchgrass ID GENE; EXPRESSION; PROMOTER; EXCISION; PLANTS; RICE; BIOMASS; ENERGY; CROP; TRANSMISSION AB Background: Switchgrass (Panicum virgatum L.) has a great potential as a platform for the production of biobased plastics, chemicals and energy mainly because of its high biomass yield on marginal land and low agricultural inputs. During the last decade, there has been increased interest in the genetic improvement of this crop through transgenic approaches. Since switchgrass, like most perennial grasses, is exclusively cross pollinating and poorly domesticated, preventing the dispersal of transgenic pollen into the environment is a critical requisite for the commercial deployment of this important biomass crop. In this study, the feasibility of controlling pollen-mediated gene flow in transgenic switchgrass using the large serine site-specific recombinase Bxb1 has been investigated. Results: A novel approach utilizing co-transformation of two separate vectors was used to test the functionality of the Bxb1/att recombination system in switchgrass. In addition, two promoters with high pollen-specific activity were identified and thoroughly characterized prior to their introduction into a test vector explicitly designed for both autoexcision and quantitative analyses of recombination events. Our strategy for developmentally programmed precise excision of the recombinase and marker genes in switchgrass pollen resulted in the generation of transgene-excised progeny. The autoexcision efficiencies were in the range of 22-42% depending on the transformation event and assay used. Conclusion: The results presented here mark an important milestone towards the establishment of a reliable biocontainment system for switchgrass which will facilitate the development of this crop as a biorefinery feedstock through advanced biotechnological approaches. C1 [Somleva, Maria N.; Xu, Chang Ai; Ryan, Kieran P.; Peoples, Oliver; Snell, Kristi D.] Metabolix Inc, Cambridge, MA 02139 USA. [Thilmony, Roger; Thomson, James] USDA ARS, CIU, Albany, CA 94710 USA. RP Somleva, MN (reprint author), Metabolix Inc, 21 Erie St, Cambridge, MA 02139 USA. EM Thomson@ARS.USDA.GOV FU Department of Energy [DE-EE0004943]; United States Government; USDA Agricultural Research Service CRIS [5325-21000-018, 5325-21000-020]; USDA - National Institute of Food and Agriculture [2010-33522-21773] FX The authors thank Dawit Abreham and Milly Nabalende for their technical assistance and R. Skadsen, USDA-ARS, for providing the Lem1 promoter. This material is based upon work supported by the Department of Energy under Award Number DE-EE0004943. This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. This research was also supported by USDA Agricultural Research Service CRIS projects 5325-21000-018 and 5325-21000-020 and by the Biotechnology Risk Assessment Program competitive grant number 2010-33522-21773 from the USDA - National Institute of Food and Agriculture. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 41 TC 1 Z9 1 U1 3 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1472-6750 J9 BMC BIOTECHNOL JI BMC Biotechnol. PD AUG 22 PY 2014 VL 14 AR 79 DI 10.1186/1472-6750-14-79 PG 13 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AN9EJ UT WOS:000340908500001 PM 25148894 ER PT J AU Ulappa, AC Kelsey, RG Frye, GG Rachlow, JL Shipley, LA Bond, L Pu, XZ Forbey, JS AF Ulappa, Amy C. Kelsey, Rick G. Frye, Graham G. Rachlow, Janet L. Shipley, Lisa A. Bond, Laura Pu, Xinzhu Forbey, Jennifer Sorensen TI Plant protein and secondary metabolites influence diet selection in a mammalian specialist herbivore SO JOURNAL OF MAMMALOGY LA English DT Article DE foraging; monoterpenes; nutrition; pygmy rabbit; sagebrush ID RABBITS BRACHYLAGUS-IDAHOENSIS; INDUCED RESISTANCE; HABITAT SELECTION; FEEDING PATTERNS; UNIFORM GARDEN; RUFFED GROUSE; QUALITY; MOOSE; CHEMISTRY; SAGEBRUSH AB For herbivores, nutrient intake is limited by the relatively low nutritional quality of plants and high concentrations of potentially toxic defensive compounds (plant secondary metabolites [PSMs]) produced by many plants. In response to phytochemical challenges, some herbivores selectively forage on plants with higher nutrient and lower PSM concentrations relative to other plants. Pygmy rabbits (Brachylagus idahoensis) are dietary specialists that feed on sagebrush (Artemisia spp.) and forage on specific plants more than others within a foraging patch. We predicted that the plants with evidence of heavy foraging (browsed plants) would be of higher dietary quality than plants that were not browsed (unbrowsed). We used model selection to determine which phytochemical variables best explained the difference between browsed and unbrowsed plants. Higher crude protein increased the odds that plants would be browsed by pygmy rabbits and the opposite was the case for certain PSMs. Additionally, because pygmy rabbits can occupy foraging patches (burrows) for consecutive years, their browsing may influence the nutritional and PSM constituents of plants at the burrows. In a post hoc analysis, we did not find a significant relationship between phytochemical concentrations, browse status, and burrow occupancy length. We concluded that pygmy rabbits use nutritional and chemical cues while making foraging decisions. C1 [Ulappa, Amy C.; Shipley, Lisa A.] Washington State Univ, Sch Environm, Pullman, WA 99164 USA. [Kelsey, Rick G.] US Forest Serv, USDA, Pacific Res Stn, Corvallis, OR 97331 USA. [Frye, Graham G.] Univ Alaska Fairbanks, Inst Arctic Biol, Fairbanks, AK 99775 USA. [Rachlow, Janet L.] Univ Idaho, Dept Fish & Wildlife Sci, Moscow, ID 83844 USA. [Bond, Laura; Pu, Xinzhu; Forbey, Jennifer Sorensen] Boise State Univ, Dept Biol Sci, Boise, ID 83725 USA. RP Ulappa, AC (reprint author), Washington State Univ, Sch Environm, 115 Johnson Hall, Pullman, WA 99164 USA. EM amy.ulappa@email.wsu.edu FU Bureau of Land Management [LO9AC16253]; National Science Foundation [DEB-1146194, IOS-1258217]; National Science Foundation Idaho Experimental Program to Stimulate Competitive Research (EPSCoR) [EPS-0814387]; Sigma-Xi Aid-in-Research Grant; Michael Butler Ecological Award; Idaho Department of Fish and Game; Boise State University FX This research was funded by the Bureau of Land Management (LO9AC16253 to JSF), the National Science Foundation (DEB-1146194 and IOS-1258217 to JSF), the National Science Foundation Idaho Experimental Program to Stimulate Competitive Research (EPSCoR) (EPS-0814387), Sigma-Xi Aid-in-Research Grant 2011, Michael Butler Ecological Award 2009, the Idaho Department of Fish and Game, and Boise State University. We appreciate the additional assistance provided by the United States Forest Service and the United States Forest Service Pacific Northwest Research Station. Special thanks to P. Campos, K. Gelken, K. DeYoung, and E. Pankau for help in the laboratory and field and to N. Wiggins and L. Felicetti for preliminary review of the manuscript. Reviewers from the Journal of Mammalogy also greatly improved this paper. NR 50 TC 9 Z9 9 U1 3 U2 45 PU ALLIANCE COMMUNICATIONS GROUP DIVISION ALLEN PRESS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-2372 EI 1545-1542 J9 J MAMMAL JI J. Mammal. PD AUG 22 PY 2014 VL 95 IS 4 BP 834 EP 842 DI 10.1644/14-MAMM-A-025 PG 9 WC Zoology SC Zoology GA AO1IG UT WOS:000341065200014 PM 26366011 ER PT J AU Warg, JV Clement, T Cornwell, ER Cruz, A Getchell, RG Giray, C Goodwin, AE Groocock, GH Faisal, M Kim, R Merry, GE Phelps, NBD Reising, MM Standish, I Zhang, Y Toohey-Kurth, K AF Warg, Janet V. Clement, Travis Cornwell, Emily R. Cruz, Angela Getchell, Rodman G. Giray, Cem Goodwin, Andrew E. Groocock, Geoffrey H. Faisal, Mohamed Kim, Robert Merry, Gwenn E. Phelps, Nicholas B. D. Reising, Monica M. Standish, Isaac Zhang, Yan Toohey-Kurth, Kathy TI Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. I. Initial comparison of four protocols SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE VHSV; Surveillance; Real-time RT-PCR; Analytical sensitivity; Analytical specificity; Validation ID ESOX-MASQUINONGY MITCHILL; POLYMERASE CHAIN-REACTION; ST-LAWRENCE-RIVER; LAKE-ONTARIO; GREAT-LAKES; GENOTYPE IVB; VHSV; ASSAY; MUSKELLUNGE; USA AB Eight laboratories worked collectively to evaluate 4 real-time RT-PCR (rRT-PCR) protocols targeting viral hemorrhagic septicemia virus (VHSV) being considered for deployment to a USA laboratory testing network. The protocols utilized previously published primers and probe sets developed for detection and surveillance of VHSV. All participating laboratories received and followed a standard operating protocol for extraction and for each of the rRT-PCR assays. Performance measures specifically evaluated included limit of detection (defined as the smallest amount of analyte in which 95% of the samples are classified as positive), analytical specificity, assay efficiency across genotype representatives, within- and between-plate variation within a laboratory, and variation between laboratories using the same platform, between platforms, and between software versions. This evaluation clearly demonstrated that the TaqMan (R)-based assay developed by Jonstrup et al. (2013; J Fish Dis 36: 9-23) produced the most consistent analytical performance characteristics for detecting all genotypes of VHSV across the 8 participating laboratories. C1 [Warg, Janet V.; Cruz, Angela] USDA, Diagnost Virol Lab, Natl Vet Serv Labs, VS,APHIS, Ames, IA 50010 USA. [Clement, Travis] S Dakota State Univ, Vet & Biomed Sci Dept, Anim Dis Res & Diagnost Lab, Brookings, SD 57007 USA. [Cornwell, Emily R.; Getchell, Rodman G.; Groocock, Geoffrey H.] Cornell Univ, Dept Microbiol & Immunol, Aquat Anim Hlth Program, Ithaca, NY 14853 USA. [Giray, Cem] Kennebec River Biosci, Richmond, ME 04357 USA. [Goodwin, Andrew E.; Merry, Gwenn E.] Univ Arkansas Pine Bluff, Aquaculture Fisheries Ctr, Pine Bluff, AR 71601 USA. [Faisal, Mohamed; Kim, Robert; Standish, Isaac] Michigan State Univ, Coll Vet Med, Aquat Anim Hlth Lab, E Lansing, MI 48824 USA. [Phelps, Nicholas B. D.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. [Reising, Monica M.] USDA, Ctr Vet Biol, VS, APHIS, Ames, IA 50010 USA. [Zhang, Yan] Ohio Dept Agr, Anim Dis Diagnost Lab, Reynoldsburg, OH 43068 USA. [Toohey-Kurth, Kathy] Univ Wisconsin, Wisconsin Vet Diagnost Lab, Madison, WI 53706 USA. RP Warg, JV (reprint author), USDA, Diagnost Virol Lab, Natl Vet Serv Labs, VS,APHIS, Ames, IA 50010 USA. EM janet.v.warg@aphis.usda.gov OI Getchell, Rodman/0000-0003-4063-4668 FU USDA FX We thank Kyle Garver of Fisheries & Oceans Canada, Pacific Biological Station, and Soren Jonstrup of the Section for Fish Diseases, National Veterinary Institute (Denmark), for sharing their testing protocols prior to publication; Elizabeth Brown and Julie Nelson of the Animal Disease Research and Diagnostic Laboratory, South Dakota State University; Gavin Glenney of the Lamar Fish Health Center, US Fish and Wildlife Service; Mary Lea Killian of the Diagnostic Virology Laboratory, National Veterinary Services Laboratories; and Gael Kurath of the USGS Western Fisheries Research Center for helpful discussions. This work was funded by the USDA. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 33 TC 6 Z9 6 U1 0 U2 6 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD AUG 21 PY 2014 VL 111 IS 1 BP 1 EP 13 DI 10.3354/dao02753 PG 13 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA AO7YN UT WOS:000341569100001 PM 25144112 ER PT J AU Warg, JV Clement, T Cornwell, ER Cruz, A Getchell, RG Giray, C Goodwin, AE Groocock, GH Faisal, M Kim, R Merry, GE Phelps, NBD Reising, MM Standish, I Zhang, Y Toohey-Kurth, K AF Warg, Janet V. Clement, Travis Cornwell, Emily R. Cruz, Angela Getchell, Rodman G. Giray, Cem Goodwin, Andrew E. Groocock, Geoffrey H. Faisal, Mohamed Kim, Robert Merry, Gwenn E. Phelps, Nicholas B. D. Reising, Monica M. Standish, Isaac Zhang, Yan Toohey-Kurth, Kathy TI Detection and surveillance of viral hemorrhagic septicemia virus using real-time RT-PCR. II. Diagnostic evaluation of two protocols SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE VHSV; Surveillance; Real-time RT-PCR; Diagnostic sensitivity; Diagnostic specificity ID NORTH-AMERICAN STRAIN; VHSV; PACIFIC; HOST; IVB AB Two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays under consideration for deployment to multiple testing laboratories across the USA were evaluated for diagnostic sensitivity and specificity on tissue homogenates obtained from natural and experimental viral hemorrhagic septicemia (VHS)-infected fish. Estimates for diagnostic specificity using virus isolation as the reference method were similar between laboratories regardless of the assay. Diagnostic sensitivity estimates of 0.96 (95% CI: 0.95, 0.97) for Jonstrup et al. (2013)'s assay (J Fish Dis 36: 9-23) exceeded the diagnostic sensitivity of 0.85 (95% CI: 0.83, 0.87) for Phelps et al. (2012)'s assay (J Aquat Anim Health 24: 238-243). The Jonstrup rRT-PCR assay is robust as demonstrated by high sensitivity and specificity estimates across laboratories and can be used as a valuable tool for targeted surveillance and for testing of suspect VHSV samples. C1 [Warg, Janet V.; Cruz, Angela] USDA, Diagnost Virol Lab, Natl Vet Serv Labs, VS,APHIS, Ames, IA 50010 USA. [Clement, Travis] S Dakota State Univ, Anim Dis Res & Diagnost Lab, Brookings, SD 57006 USA. [Cornwell, Emily R.; Getchell, Rodman G.; Groocock, Geoffrey H.] Cornell Univ, Dept Microbiol & Immunol, Aquat Anim Hlth Program, Ithaca, NY 14853 USA. [Giray, Cem] Kennebec River Biosci, Richmond, ME 04357 USA. [Goodwin, Andrew E.; Merry, Gwenn E.] Univ Arkansas Pine Bluff, Aquaculture Fisheries Ctr, Pine Bluff, AR 71601 USA. [Faisal, Mohamed; Kim, Robert; Standish, Isaac] Michigan State Univ, Coll Vet Med, Aquat Anim Hlth Lab, E Lansing, MI 48824 USA. [Phelps, Nicholas B. D.] Univ Minnesota, Vet Diagnost Lab, St Paul, MN 55108 USA. [Reising, Monica M.] USDA, Ctr Vet Biol, VS, APHIS, Ames, IA 50010 USA. [Zhang, Yan] Ohio Dept Agr, Anim Dis Diagnost Lab, Reynoldsburg, OH 43068 USA. [Toohey-Kurth, Kathy] Univ Wisconsin, Wisconsin Vet Diagnost Lab, Madison, WI 53706 USA. RP Warg, JV (reprint author), USDA, Diagnost Virol Lab, Natl Vet Serv Labs, VS,APHIS, Ames, IA 50010 USA. EM janet.v.warg@aphis.usda.gov OI Getchell, Rodman/0000-0003-4063-4668 FU USDA FX We thank Soren Jonstrup, formerly of the Section for Fish Diseases, National Veterinary Institute (Denmark), for sharing his testing protocols prior to publication; Paul Hershberger of the Marrowstone Marine Field Station, United States Geological Survey, for providing VHSV IVa positive tissues; and Mary Lea Killian of the Diagnostic Virology Laboratory, National Veterinary Services Laboratories, and Gael Kurath of the USGS Western Fisheries Research Center, for helpful discussions. This work was funded by USDA. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the US Government. NR 17 TC 5 Z9 5 U1 1 U2 7 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD AUG 21 PY 2014 VL 111 IS 1 BP 15 EP 22 DI 10.3354/dao02758 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA AO7YN UT WOS:000341569100002 PM 25144113 ER PT J AU Nayduch, D Lee, MB Saski, CA AF Nayduch, Dana Lee, Matthew B. Saski, Christopher A. TI Gene discovery and differential expression analysis of humoral immune response elements in female Culicoides sonorensis (Diptera: Ceratopogonidae) SO PARASITES & VECTORS LA English DT Article DE Innate immunity; Biting midge; Toll; Imd; JAK/STAT; RNAseq; Attacin; Cecropin; Defensin ID MOSQUITO ANOPHELES-GAMBIAE; ANTIMICROBIAL PEPTIDES; VECTOR COMPETENCE; NEGATIVE REGULATION; SIGNALING PATHWAY; INNATE IMMUNITY; AEDES-AEGYPTI; HOST-DEFENSE; DROSOPHILA; TOLL AB Background: Female Culicoides sonorensis midges (Diptera: Ceratopogonidae) are vectors of pathogens that impact livestock and wildlife in the United States. Little is known about their biology on a molecular-genetic level, including components of their immune system. Because the insect immune response is involved with important processes such as gut microbial homeostasis and vector competence, our aims were to identify components of the midge innate immune system and examine their expression profiles in response to diet across time. Methods: In our previous work, we de novo sequenced and analyzed the transcriptional landscape of female midges under several feeding states including teneral (unfed) and early and late time points after blood and sucrose. Here, those transcriptomes were further analyzed to identify insect innate immune orthologs, particularly humoral immune response elements. Additionally, we examined immune gene expression profiles in response to diet over time, on both a transcriptome-wide, whole-midge level and more specifically via qRTPCR analysis of antimicrobial peptide (AMP) expression in the alimentary canal. Results: We identified functional units comprising the immune deficiency (Imd), Toll and JAK/STAT pathways, including humoral factors, transmembrane receptors, signaling components, transcription factors/regulators and effectors such as AMPs. Feeding altered the expression of receptors, regulators, AMPs, prophenoloxidase and thioester-containing proteins, where blood had a greater effect than sucrose on the expression profiles of most innate immune components. qRTPCR of AMP genes showed that all five were significantly upregulated in the alimentary canal after blood feeding, possibly in response to proliferating populations of gut bacteria. Conclusions: Identification and functional insight of humoral/innate immune components in female C. sonorensis updates our knowledge of the molecular biology of this important vector. Because diet alone influenced the expression of immune pathway components, including their effectors, subsequent study of the role of innate immunity in biological processes such as gut homeostasis and life history are being pursued. Furthermore, since the humoral response is a key contributor in gut immunity, manipulating immune gene expression will help in uncovering genetic components of vector competence, including midgut barriers to infection. The results of such studies will serve as a platform for designing novel transmission-blocking strategies. C1 [Nayduch, Dana; Lee, Matthew B.] USDA ARS, Ctr Grain & Anim Hlth Res, Arthropod Borne Anim Dis Res Unit, Manhattan, KS 66502 USA. [Saski, Christopher A.] Clemson Univ, Genom & Computat Biol Lab, Inst Translat Genom, Clemson, SC 29634 USA. RP Nayduch, D (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, Arthropod Borne Anim Dis Res Unit, 1515 Coll Ave, Manhattan, KS 66502 USA. EM dana.nayduch@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service; National Program 104: Veterinary, Medical and Urban Entomology [5430-32000-003]; Clemson University [58-5430-2-313] FX DN and MBL would like to thank J. Glenn, J. Kempert and B. Yarnell of ABADRU for midge rearing and manipulation assistance. This work was funded by the U.S. Department of Agriculture, Agricultural Research Service, National Program 104: Veterinary, Medical and Urban Entomology, Project Number 5430-32000-003, and a Specific Cooperative Agreement with Clemson University, Agreement Number 58-5430-2-313. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the USDA. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the USDA. NR 63 TC 5 Z9 5 U1 3 U2 34 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD AUG 21 PY 2014 VL 7 AR 388 DI 10.1186/1756-3305-7-388 PG 17 WC Parasitology SC Parasitology GA AO3BN UT WOS:000341203200001 PM 25145345 ER PT J AU Lin, ZY Xu, SQ Que, YX Wang, JH Comstock, JC Wei, JJ McCord, PH Chen, BS Chen, RK Zhang, MQ AF Lin, Zhenyue Xu, Shiqiang Que, Youxiong Wang, Jihua Comstock, Jack C. Wei, Jinjin McCord, Per H. Chen, Baoshan Chen, Rukai Zhang, Muqing TI Species-Specific Detection and Identification of Fusarium Species Complex, the Causal Agent of Sugarcane Pokkah Boeng in China SO PLOS ONE LA English DT Article ID REAL-TIME PCR; VERTICILLIOIDES; QUANTIFICATION; PATHOGENICITY; PROLIFERATUM; DIVERSITY; FUJIKUROI; SEQUENCES; DIAGNOSIS; STRAINS AB Background: Pokkah boeng disease caused by the Fusarium species complex results in significant yield losses in sugarcane. Thus, the rapid and accurate detection and identification of the pathogen is urgently required to manage and prevent the spreading of sugarcane pokkah boeng. Methods: A total of 101 isolates were recovered from the pokkah boeng samples collected from five major sugarcane production areas in China throughout 2012 and 2013. The causal pathogen was identified by morphological observation, pathogenicity test, and phylogenetic analysis based on the fungus-conserved rDNA-ITS. Species-specific TaqMan real-time PCR and conventional PCR methods were developed for rapid and accurate detection of the causal agent of sugarcane pokkah boeng. The specificity and sensitivity of PCR assay were also evaluated on a total of 84 isolates of Fusarium from China and several isolates from other fungal pathogens of Sporisorium scitamineum and Phoma sp. and sugarcane endophyte of Acremonium sp. Result: Two Fusarium species (F. verticillioides and F. proliferatum) that caused sugarcane pokahh boeng were identified by morphological observation, pathogenicity test, and phylogenetic analysis. Species-specific TaqMan PCR and conventional PCR were designed and optimized to target their rDNA-ITS regions. The sensitivity of the TaqMan PCR was approximately 10 pg of fungal DNA input, which was 1,000-fold over conventional PCR, and successfully detected pokkah boeng in the field-grown sugarcane. Conclusions/Significance: This study was the first to identify two species, F. verticillioides and F. proliferatum, that were causal pathogens of sugarcane pokkah boeng in China. It also described the development of a species-specific PCR assay to detect and confirm these pathogens in sugarcane plants from mainland China. This method will be very useful for a broad range of research endeavors as well as the regulatory response and management of sugarcane pokkah boeng. C1 [Lin, Zhenyue; Xu, Shiqiang; Wang, Jihua; Chen, Baoshan; Zhang, Muqing] Guangxi Univ, Nanning 530004, Guangxi, Peoples R China. [Lin, Zhenyue; Que, Youxiong; Wei, Jinjin; Chen, Rukai; Zhang, Muqing] Fujian Agr & Forestry Univ, Fuzhou, Peoples R China. [Comstock, Jack C.; McCord, Per H.] ARS, USDA, Sugarcane Field Stn, Canal Point, FL USA. RP Zhang, MQ (reprint author), Guangxi Univ, Nanning 530004, Guangxi, Peoples R China. EM mqzhang@ufl.edu FU Guangxi special funding for distinguished experts and Funds from State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources; Guangxi Natural Science Foundation [2014GXNSFFA118002] FX Financial support was provided by Guangxi special funding for distinguished experts and Funds from State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources. This project is also supported by Guangxi Natural Science Foundation (2014GXNSFFA118002). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 6 Z9 8 U1 0 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 20 PY 2014 VL 9 IS 8 AR e104195 DI 10.1371/journal.pone.0104195 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AQ3JB UT WOS:000342687200015 PM 25141192 ER PT J AU Pandey, MK Upadhyaya, HD Rathore, A Vadez, V Sheshshayee, MS Sriswathi, M Govil, M Kumar, A Gowda, MVC Sharma, S Hamidou, F Kumar, VA Khera, P Bhat, RS Khan, AW Singh, S Li, HJ Monyo, E Nadaf, HL Mukri, G Jackson, SA Guo, BZ Liang, XQ Varshney, RK AF Pandey, Manish K. Upadhyaya, Hari D. Rathore, Abhishek Vadez, Vincent Sheshshayee, M. S. Sriswathi, Manda Govil, Mansee Kumar, Ashish Gowda, M. V. C. Sharma, Shivali Hamidou, Falalou Kumar, V. Anil Khera, Pawan Bhat, Ramesh S. Khan, Aamir W. Singh, Sube Li, Hongjie Monyo, Emmanuel Nadaf, H. L. Mukri, Ganapati Jackson, Scott A. Guo, Baozhu Liang, Xuanqiang Varshney, Rajeev K. TI Genomewide Association Studies for 50 Agronomic Traits in Peanut Using the 'Reference Set' Comprising 300 Genotypes from 48 Countries of the Semi-Arid Tropics of the World SO PLOS ONE LA English DT Article ID ARACHIS-HYPOGAEA L.; SIMPLE SEQUENCE REPEAT; MINI-CORE COLLECTION; LINKAGE DISEQUILIBRIUM; POPULATION-STRUCTURE; MICROSATELLITE MARKERS; CULTIVATED PEANUT; RUST RESISTANCE; WINTER-WHEAT; SSR MARKERS AB Peanut is an important and nutritious agricultural commodity and a livelihood of many small-holder farmers in the semi-arid tropics (SAT) of world which are facing serious production threats. Integration of genomics tools with on-going genetic improvement approaches is expected to facilitate accelerated development of improved cultivars. Therefore, high-resolution genotyping and multiple season phenotyping data for 50 important agronomic, disease and quality traits were generated on the 'reference set' of peanut. This study reports comprehensive analyses of allelic diversity, population structure, linkage disequilibrium (LD) decay and marker-trait association (MTA) in peanut. Distinctness of all the genotypes can be established by using either an unique allele detected by a single SSR or a combination of unique alleles by two or more than two SSR markers. As expected, DArT features (2.0 alleles/locus, 0.125 PIC) showed lower allele frequency and polymorphic information content (PIC) than SSRs (22.21 alleles /locus, 0.715 PIC). Both marker types clearly differentiated the genotypes of diploids from tetraploids. Multi-allelic SSRs identified three sub-groups (K = 3) while the LD simulation trend line based on squared-allele frequency correlations (r(2)) predicted LD decay of 15-20 cM in peanut genome. Detailed analysis identified a total of 524 highly significant MTAs (pvalue.2.1610-6) with wide phenotypic variance (PV) range (5.81-90.09%) for 36 traits. These MTAs after validation may be deployed in improving biotic resistance, oil/seed/nutritional quality, drought tolerance related traits, and yield/yield components. C1 [Pandey, Manish K.; Upadhyaya, Hari D.; Rathore, Abhishek; Vadez, Vincent; Sriswathi, Manda; Govil, Mansee; Kumar, Ashish; Sharma, Shivali; Kumar, V. Anil; Khera, Pawan; Khan, Aamir W.; Singh, Sube; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Telangana, India. [Sheshshayee, M. S.] Univ Agr Sci, Dept Crop Physiol, Bangalore, Karnataka, India. [Kumar, Ashish] Jawahar Lal Nehru Krishi Vishwa Vidyalaya JNKVV, Plant Pathol, Jabalpur, Madhya Pradesh, India. [Gowda, M. V. C.; Nadaf, H. L.; Mukri, Ganapati] Univ Agr Sci, Dept Genet & Plant Breeding, Dharwad, Karnataka, India. [Hamidou, Falalou] Int Crops Res Inst Semi Arid Trop, Sahelian Ctr, Niamey, Niger. [Bhat, Ramesh S.] Univ Agr Sci, Dept Biotechnol, Dharwad, Karnataka, India. [Li, Hongjie] ShanDong Shofine Seed Technol Co Ltd, Jining, Shandong, Peoples R China. [Monyo, Emmanuel] Int Crops Res Inst Semi Arid Trop, Nairobi, Kenya. [Jackson, Scott A.] Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA. [Guo, Baozhu] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Liang, Xuanqiang] GAAS, Crop Res Inst, Guangzhou, Guangdong, Peoples R China. RP Varshney, RK (reprint author), Int Crops Res Inst Semi Arid Trop, Hyderabad, Telangana, India. EM r.k.varshney@cgiar.org RI Updhayaya, Hari/C-4858-2014; Bhat, Ramesh/C-8080-2014; Varshney, Rajeev/C-5295-2014; Vadez, Vincent/C-5310-2014 OI Updhayaya, Hari/0000-0002-5166-6844; Varshney, Rajeev/0000-0002-4562-9131; Vadez, Vincent/0000-0003-2014-0281 FU Bill & Melinda Gates Foundation (BMGF) through CGIAR Generation Challenge Programme; Peanut Foundation, USA; ShanDong Shofine Seed Technology Co Ltd, Jining, Shandong, China FX This study was undertaken as a part of Tropical Legumes I (TLI-Objective 1: Groundnut) funded by Bill & Melinda Gates Foundation (BMGF) through CGIAR Generation Challenge Programme (http://www.generationcp.org/) and The Peanut Foundation, USA (http://www.peanutfoundation.org/). This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. ShanDong Shofine Seed Technology Co Ltd, Jining, Shandong, China provided support in the form of a salary for author HL who participated in data analysis using analytical tools, but did not have any additional role in the study design and data collection, decision to publish, or preparation of the manuscript. The specific roles of the authors are articulated in the "author contributions" section. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 48 TC 12 Z9 12 U1 2 U2 16 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 20 PY 2014 VL 9 IS 8 AR e105228 DI 10.1371/journal.pone.0105228 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AQ3JB UT WOS:000342687200064 PM 25140620 ER PT J AU Hauptman, BS Barrows, FT Block, SS Gaylord, TG Paterson, JA Rawles, SD Sealey, WM AF Hauptman, Blake S. Barrows, Frederic T. Block, Stephanie S. Gaylord, T. Gibson Paterson, John A. Rawles, Steven D. Sealey, Wendy M. TI Evaluation of grain distillers dried yeast as a fish meal substitute in practical-type diets of juvenile rainbow trout, Oncorhynchus mykiss SO AQUACULTURE LA English DT Article DE Alternative protein; Rainbow trout; Grain distillers dried yeast; Fish nutrition ID APPARENT DIGESTIBILITY; ETHANOL YEAST; SUNSHINE BASS; AMINO-ACIDS; PROTEIN; FEEDS; INGREDIENTS; REPLACEMENT; PERFORMANCE; AQUAFEEDS AB Grain distillers dried yeast (GDDY) is a single-cell protein obtained as a co-product during the production of fuel ethanol that may have potential as a protein replacement for rainbow trout. The goal of this study was to examine the suitability of GDDY as a replacement for fish meal on a digestible protein basis in rainbow trout diets. An in-vivo digestibility study was performed to determine the nutrient availability of GDDY. Subsequently, a control diet containing 42% digestible protein and 20% lipid was formulated to replace fish meal protein with GDDY protein at eight different levels (0, 25, 375, 50, 62.5, 75, 875, and 100%). Diets were fed to juvenile rainbow trout stocked into four replicate tanks per dietary treatment (30 fish/tank) and fed twice daily for nine weeks. High GDDY inclusion rates significantly altered rainbow trout growth and feed conversion but not feed intake. There were no significant differences in production performance in fish fed the 25% GDDY and 37.5% GDDY diets when compared to fish fed the control diet, but further dietary fish meal replacement generally decreased fish performance. Further research is warranted to determine why fish performance decreased with higher inclusion levels of GDDY in spite of similar feed intake among levels. Published by Elsevier B.V. C1 [Hauptman, Blake S.; Gaylord, T. Gibson; Sealey, Wendy M.] US Fish & Wildlife Serv, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Hauptman, Blake S.; Paterson, John A.] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59715 USA. [Barrows, Frederic T.] ARS, USDA, Trout Grains Project, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Block, Stephanie S.] ADM Res, Decatur, IL 62521 USA. [Rawles, Steven D.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. RP Sealey, WM (reprint author), US Fish & Wildlife Serv, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. EM Wendy_Sealey@fws.gov NR 35 TC 7 Z9 7 U1 2 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD AUG 20 PY 2014 VL 432 SI SI BP 7 EP 14 DI 10.1016/j.aquaculture.2014.03.026 PG 8 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AP7HA UT WOS:000342247200002 ER PT J AU Read, ES Barrows, FT Gaylord, TG Paterson, J Petersen, MK Sealey, WM AF Read, Elizabeth S. Barrows, Frederic T. Gaylord, T. Gibson Paterson, John Petersen, M. K. Sealey, Wendy M. TI Investigation of the effects of dietary protein source on copper and zinc bioavailability in fishmeal and plant-based diets for rainbow trout SO AQUACULTURE LA English DT Article DE Copper; Zinc; Plant-based diets; Rainbow trout ID ONCORHYNCHUS-MYKISS; MINERAL REQUIREMENTS; GROWTH EFFICIENCY; SALMO-GAIRDNERI; SUPPLEMENTATION; METABOLISM; CALCIUM; LEVEL; CARP AB Limited research has examined the effects that dietary protein sources have on copper (Cu) and zinc (Zn) absorption, interactions and utilization in rainbow trout. Therefore, the objective of the first trial was to determine what effect Cu source (complex vs. inorganic) and Cu concentration (0, 5, 10, 15,20 ppm) had on rate and efficiency of gain and Cu tissue concentration in rainbow trout fed both plant-protein and animal-protein based diets. The second trial then examined if interactions occur due to increasing dietary content of Zn (0, 30, 300, 1500 ppm) in rainbow trout fed a plant-based diet. Results from the first trial demonstrated differences in growth and tissue mineral concentrations due to Cu concentration but not source. Trout fed plant-based diets had higher weight gain, improved feed conversion ratio and higher hepatic Cu concentration when compared to fishmeal-fed trout. Results from the second trial show that increasing dietary Zn supplementation increased whole body Zn and Cu at 12 weeks. No significant antagonism between Cu and Zn was observed in rainbow trout fed plant-based diets. Zn deficiency signs were observed at 12 weeks in trout fed a plant-based diet without Cu and Zn supplementation which included mortality, cataracts and caudal fin erosion. Results of these studies indicate that rainbow trout fed plant-based diets require both Cu and Zn supplementation at concentrations higher than those previously reported to maximize growth. (C) Published by Elsevier B.V. C1 [Read, Elizabeth S.; Gaylord, T. Gibson; Sealey, Wendy M.] USFWS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Read, Elizabeth S.; Paterson, John] Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59715 USA. [Barrows, Frederic T.] ARS, USDA, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. [Petersen, M. K.] ARS, USDA, Ft Keogh Livestock & Range Res Lab, Miles City, MT 59301 USA. RP Sealey, WM (reprint author), USFWS, Bozeman Fish Technol Ctr, Bozeman, MT 59715 USA. EM Wendy_Sealey@fws.gov NR 34 TC 5 Z9 5 U1 1 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD AUG 20 PY 2014 VL 432 SI SI BP 97 EP 105 DI 10.1016/j.aquaculture.2014.04.029 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AP7HA UT WOS:000342247200013 ER PT J AU Vallejo, RL Palti, Y Liu, SX Marancik, DP Wiens, GD AF Vallejo, Roger L. Palti, Yniv Liu, Sixin Marancik, David P. Wiens, Gregory D. TI Validation of linked QTL for bacterial cold water disease resistance and spleen size on rainbow trout chromosome Omy19 SO AQUACULTURE LA English DT Article DE Flavobacterium psychrophilum; Bacterial cold water disease; Rainbow trout; Disease resistance; Spleen ID FLAVOBACTERIUM-PSYCHROPHILUM; ONCORHYNCHUS-MYKISS; GENETIC-LINKAGE; MAP; PEDIGREES; SURVIVAL; GENOME AB Bacterial cold water disease (BCWD) is caused by infection with Flavobacterium psychrophilum, and outbreaks cause significant economic losses in salmonid aquaculture. Previously, we identified a major QTL for BOND resistance on rainbow trout chromosome Omy19 as well as a QTL for spleen size (SPLW = spleen weight and SPLI = spleen index) which in naive fish is a surrogate indicator of BCWD resistance. The objective of this study was to validate the BOND resistance and spleen size QTL in a subsequent generation and investigate whether the correlation between BCWD resistance and spleen size loci on Omy19 was due to close linkage or pleiotropy. We designed four full-sib matings whose offspring were evaluated for BCWD resistance, body weight, SPLW and SPLI. A total of 800 offspring were genotyped with seven microsatellite markers from Omy19. Our analyses confirmed the presence of QTL for BOND and spleen size on Omy19. Our findings also suggest that BOND resistance and spleen size are not influenced by a single pleiotropic QTL on Omy19 as the QTL were separately inherited in the families studied. These data increase our understanding of the genetic basis of rainbow trout BOND resistance. Published by Elsevier B.V. C1 [Vallejo, Roger L.; Palti, Yniv; Liu, Sixin; Marancik, David P.; Wiens, Gregory D.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Vallejo, RL (reprint author), USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, 11861 Leetown Rd, Kearneysville, WV 25430 USA. EM roger.vallejo@ars.usda.gov FU Agricultural Research Service CRIS [1930-32000-005]; CRIS [1930-32000-009] FX The authors thank Joel Caren, Travis Moreland, Kristy Shewbridge, Lakyn Sanders and Roseanna Long for providing technical assistance. We also thank Dr. Guangtu Gao for streamlining the genotyping analysis pipeline. We are grateful to Dr. Per Madsen for help in estimating genetic correlation between BCWD resistance and spleen size phenotypes performing Bayesian linear modeling with software DMU. This work was supported by the Agricultural Research Service CRIS Project 1930-32000-005 "Host-Pathogen and Environmental Interactions in Cool and Cold Water Aquaculture" and CRIS Project 1930-32000-009 "Utilization of Genomics for Improving Production Traits in Cool and Cold Water Aquaculture". Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S Department of Agriculture. USDA is an equal opportunity provider and employer. NR 24 TC 7 Z9 8 U1 1 U2 18 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD AUG 20 PY 2014 VL 432 SI SI BP 139 EP 143 DI 10.1016/j.aquaculture.2014.05.003 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AP7HA UT WOS:000342247200019 ER PT J AU Ali, SS Gunupuru, LR Kumar, GBS Khan, M Scofield, S Nicholson, P Doohan, FM AF Ali, Shahin S. Gunupuru, Lokanadha R. Kumar, G. B. Sunil Khan, Mojibur Scofield, Steve Nicholson, Paul Doohan, Fiona M. TI Plant disease resistance is augmented in uzu barley lines modified in the brassinosteroid receptor BRI1 SO BMC PLANT BIOLOGY LA English DT Article DE Disease resistance; Brassinosteroid; Uzu; BRI1; Fusarium ID HEAD BLIGHT DISEASE; GENE-EXPRESSION; FUSARIUM-GRAMINEARUM; KINASE BAK1; WHEAT; ARABIDOPSIS; RICE; BIOSYNTHESIS; ACTIVATION; GROWTH AB Background: Brassinosteroid hormones regulate many aspects of plant growth and development. The membrane receptor BRI1 is a central player in the brassinosteroid signaling cascade. Semi-dwarf 'uzu' barley carries a mutation in a conserved domain of the kinase tail of BRI1 and this mutant allele is recognised for its positive contribution to both yield and lodging resistance. Results: Here we show that uzu barley exhibits enhanced resistance to a range of pathogens. It was due to a combination of preformed, inducible and constitutive defence responses, as determined by a combination of transcriptomic and biochemical studies. Gene expression studies were used to determine that the uzu derivatives are attenuated in downstream brassinosteroid signaling. The reduction of BRI1 RNA levels via virus-induced gene silencing compromised uzu disease resistance. Conclusions: The pathogen resistance of uzu derivatives may be due to pleiotropic effects of BRI1 or the cascade effects of their repressed BR signaling. C1 [Ali, Shahin S.; Gunupuru, Lokanadha R.; Kumar, G. B. Sunil; Khan, Mojibur; Doohan, Fiona M.] Univ Coll Dublin, Mol Plant Microbe Interact Lab, Sch Biol & Environm Sci, Dublin 4, Ireland. [Scofield, Steve] Purdue Univ, USDA ARS, Dept Agron, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. [Scofield, Steve] Purdue Univ, W Lafayette, IN 47907 USA. [Nicholson, Paul] John Innes Inst, Dept Crop Genet, Norwich NR4 7UH, Norfolk, England. [Ali, Shahin S.] USDA ARS, SPCL, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. RP Ali, SS (reprint author), Univ Coll Dublin, Mol Plant Microbe Interact Lab, Sch Biol & Environm Sci, Dublin 4, Ireland. EM shahinsharif.ali@gmail.com FU Science Foundation Ireland research fund [IN10/IN.1/B3028]; Department of Agriculture Research Stimulus Grant [RSF 07 513] FX This work was supported by the Science Foundation Ireland research fund (IN10/IN.1/B3028) and Department of Agriculture Research Stimulus Grant RSF 07 513. We thank K. Sato, Barley Germplasm Centre, Okayama University, Japan for providing seeds of Akashinriki and its uzu derivative. NR 52 TC 6 Z9 6 U1 1 U2 35 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 20 PY 2014 VL 14 AR 227 DI 10.1186/s12870-014-0227-1 PG 15 WC Plant Sciences SC Plant Sciences GA AO4NX UT WOS:000341318100001 PM 25135116 ER PT J AU Alvarado-Esquivel, C Romero-Salas, D Cruz-Romero, A Garcia-Vazquez, Z Peniche-Cardena, A Ibarra-Priego, N Ahuja-Aguirre, C Perez-de-Leon, AA Dubey, JP AF Alvarado-Esquivel, Cosme Romero-Salas, Dora Cruz-Romero, Anabel Garcia-Vazquez, Zeferino Peniche-Cardena, Alvaro Ibarra-Priego, Nelly Ahuja-Aguirre, Concepcion Perez-de-Leon, Adalberto A. Dubey, Jitender P. TI High prevalence of Toxoplasma gondii antibodies in dogs in Veracruz, Mexico SO BMC VETERINARY RESEARCH LA English DT Article DE Toxoplasma gondii; Dogs; Modified agglutination test; Antibodies; Prevalence; Mexico ID NEOSPORA-CANINUM; STRAY DOGS; DURANGO; OOCYSTS AB Background: Little is known concerning the prevalence of Toxoplasma gondii infection in dogs in Mexico. Here, we investigated antibodies to T. gondii and associated risk factors in 101 dogs from an animal shelter in Veracruz State, Mexico. Canine sera were assayed for T. gondii IgG antibodies by using the modified agglutination test (MAT, cut off 1:25). Results: Sixty eight (67.3%) of 101 dogs were seropositive with titers of 1:25 in 16, 1:50 in 8, 1:100 in 9, 1:200 in 10, 1:400 in 10, 1:800 in 10, 1:1600 in 3, and 1:3200 or higher in 2. None of the dogs' characteristics studied including age, sex, breed, and history of deworming, rabies vaccination and contact with cats was associated with seroprevalence of T. gondii infection. Conclusion: Using the dogs as sentinel animals, the results indicate high contamination with T. gondii of the environment in Veracruz, Mexico. Results have public health implications, and further studies in Veracruz should be conducted to establish the sources of environmental contamination with T. gondii and to determine optimal preventive measures against T. gondii infection in humans. C1 [Alvarado-Esquivel, Cosme] Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Durango 34000, Mexico. [Romero-Salas, Dora; Cruz-Romero, Anabel; Peniche-Cardena, Alvaro; Ibarra-Priego, Nelly; Ahuja-Aguirre, Concepcion] Univ Veracruzana, Fac Med Vet & Zootecnia, Lab Parasitol, Xapala 91710, Veracruz, Mexico. [Garcia-Vazquez, Zeferino] INIFAP, Ctr Nacl Invest Disciplinaria Parasitol Vet, Jiutepec 6225, Morelos, Mexico. [Perez-de-Leon, Adalberto A.] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Dubey, Jitender P.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Alvarado-Esquivel, Cosme] Fac Med & Nutr, Lab Invest Biomed, Durango 34000, Mexico. RP Alvarado-Esquivel, C (reprint author), Juarez Univ Durango State, Fac Med & Nutr, Biomed Res Lab, Ave Univ S-N, Durango 34000, Mexico. EM alvaradocosme@yahoo.com FU Juarez University of Durango State, Mexico FX USDA is an equal opportunity provider and employer. This research project was financed in part by Juarez University of Durango State, Mexico. NR 13 TC 6 Z9 6 U1 0 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD AUG 20 PY 2014 VL 10 AR 191 DI 10.1186/s12917-014-0191-x PG 4 WC Veterinary Sciences SC Veterinary Sciences GA AN9FD UT WOS:000340910600001 PM 25134696 ER PT J AU Gomez-Zorita, S Fernandez-Quintela, A Lasa, A Aguirre, L Rimando, AM Portillo, MP AF Gomez-Zorita, Saioa Fernandez-Quintela, Alfredo Lasa, Arrate Aguirre, Leixuri Rimando, Agnes M. Portillo, Maria P. TI Pterostilbene, a Dimethyl Ether Derivative of Resveratrol, Reduces Fat Accumulation in Rats Fed an Obesogenic Diet SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE adipose tissue; bioavailability; fatty acid oxidation; lipogenesis; pterostilbene ID TRANS-RESVERATROL; 3T3-L1 ADIPOCYTES; SKELETAL-MUSCLE; ADIPOSE-TISSUE; METABOLISM; ACID; HAMSTERS; CELLS; LIVER; SIRT1 AB The current study aimed to demonstrate the effects of pterostilbene in rats fed an obesogenic diet. For this purpose, pterostilbene was administered at doses of 15 mg/kg body weight/day (PT15 group) or 30 mg/kg body weight/day (PT30 group) for 6 weeks. Pterostilbene reduced adipose tissue mass -15.1% (PT15) and -22.9% (PT30). In this tissue, it decreased malic enzyme (-39.4 and 49.5% for PT15 and PT30 groups, respectively) and fatty acid synthase (-45 and 53.4% for PT15 and PT30) activities. Acetyl-CoA carboxylase activity was reduced and AMPK activity was increased only in the PT30 group. In the liver, pterostilbene (PT30) reduced malic enzyme (-29.5%) and glucose-6-P dehydrogenase (-43.2%) activities and increased carnitine palmitoyltransferase-1a (37.5%) and acyl-coenzyme A oxidase (42.5%) activities. This increased oxidative capacity was not associated with increased mitochondriogenesis. Among biochemical serum parameters, only insulin was modified by pterostilbene (-31.6%) in the PT15 group. The amounts of pterostilbene in serum and tissues from rats in the PT30 group were in not all cases 2-fold greater than those found in the PT15 group. In conclusion, pterostilbene shows antiobesity properties due, at least in part, to reduced lipogenesis in adipose tissue and increased fatty acid oxidation in liver. C1 [Gomez-Zorita, Saioa; Fernandez-Quintela, Alfredo; Lasa, Arrate; Aguirre, Leixuri; Portillo, Maria P.] Univ Basque Country UPV EHU, Dept Nutr & Food Sci, Nutr & Obes Grp, Vitoria 01006, Spain. [Gomez-Zorita, Saioa; Fernandez-Quintela, Alfredo; Lasa, Arrate; Aguirre, Leixuri; Portillo, Maria P.] Lucio Lascaray Res Ctr, Vitoria 01006, Spain. [Gomez-Zorita, Saioa; Fernandez-Quintela, Alfredo; Lasa, Arrate; Aguirre, Leixuri; Portillo, Maria P.] Inst Hlth Carlos III ISCIII, CIBERobn Physiopathol Obes & Nutr, Madrid, Spain. [Rimando, Agnes M.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS USA. RP Portillo, MP (reprint author), Fac Pharm, Dept Nutr & Food Sci, Paseo Univ 7, Vitoria 01006, Spain. EM mariapuy.portillo@ehu.es RI Lasa, Arrate/L-7549-2014; OI Lasa, Arrate/0000-0003-2399-4979; LASA ELGUEZUA, ARRATE/0000-0002-8309-8212; PORTILLO BAQUEDANO, MARIA PUY/0000-0003-2290-592X; Fernandez-Quintela, Alfredo/0000-0003-2709-4118 FU Ministerio de Ciencia e Innovacion [AGL2008-01005-ALI]; Ministerio de Economia y Competitividad [AGL2011-27406-ALI]; Instituto de Salud Carlos III (CIBERObn); government of the Basque Country [IT-512-13]; University of the Basque Country (UPV/EHU) [ELDUNANOTEK UFI11/32]; University of the Basque Country (UPV/EHU) FX This study was supported by grants from the Ministerio de Ciencia e Innovacion (AGL2008-01005-ALI) and Ministerio de Economia y Competitividad (AGL2011-27406-ALI), Instituto de Salud Carlos III (CIBERObn), the government of the Basque Country (IT-512-13), and the University of the Basque Country (UPV/EHU) (ELDUNANOTEK UFI11/32). S.G.-Z. is the recipient of a doctoral fellowship from the University of the Basque Country (UPV/EHU). NR 39 TC 9 Z9 9 U1 4 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 20 PY 2014 VL 62 IS 33 BP 8371 EP 8378 DI 10.1021/jf501318b PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AN6WI UT WOS:000340738700018 PM 25083823 ER PT J AU Das, N Berhow, MA Angelino, D Jeffery, EH AF Das, Nilanjan Berhow, Mark A. Angelino, Donato Jeffery, Elizabeth H. TI Camelina sativa Defatted Seed Meal Contains Both Alkyl Sulfinyl Glucosinolates and Quercetin That Synergize Bioactivity SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE camelina; glucosinolate; quercetin; NQO1; synergy; sulforaphane ID QUINONE REDUCTASE; CHROMATOGRAPHY; PURIFICATION; CYP1A1; CHEMOPREVENTION; SULFORAPHANE; FLAVONOIDS; CELLS; ASSAY AB Camelina sativa L. Crantz is under development as a novel oilseed crop, yet bioefficacy of camelina phytochemicals is unknown. Defatted camelina seed meal contains two major aliphatic glucosinolates (GSLs), glucoarabin (9-(methylsulfinyl)nonylglucosinolate; GSL 9) and glucocamelinin (10-(methylsulfinyl)decylglucosinolate; GSL 10), with traces of a third, 11(methylsulfinyl)undecylglucosinolate and several flavonoids, mostly quercetin glycosides. In Hepa1c1c7 cells, hydrolyzed GSLs (hGSLs) 9 and 10 upregulated the phase II detoxification enzyme quinone reductase (NQO1), with no effect on cytochrome P450 (CYP) 1A1 activity. Isobologram graphs revealed synergy of NQO1 induction for a combination of hGSL 9 and quercetin. These findings suggest that defatted camelina seed meal should be evaluated for anticancer activity, similar to broccoli and other Brassicaceae family members. Interestingly, synergy of NQO1 induction was also seen for physiologically relevant doses of sulforaphane (SF) and quercetin, two key bioactives present in broccoli. This suggests that SF within broccoli may be more potent than purified SF. C1 [Das, Nilanjan; Angelino, Donato; Jeffery, Elizabeth H.] Univ Illinois, Dept Food Sci & Human Nutr, Urbana, IL 61801 USA. [Berhow, Mark A.] ARS, USDA, Peoria, IL 61604 USA. RP Jeffery, EH (reprint author), Univ Illinois, Dept Food Sci & Human Nutr, 905 S Goodwin Ave, Urbana, IL 61801 USA. EM ejeffery@illinois.edu RI Angelino, Donato/G-7894-2015 OI Angelino, Donato/0000-0002-5436-7428 FU United States Department of Agriculture [USDA/NIFA 2010-65200-20398] FX This work was funded by a grant to E.H.J. from the United States Department of Agriculture (USDA/NIFA 2010-65200-20398). NR 26 TC 2 Z9 2 U1 2 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 20 PY 2014 VL 62 IS 33 BP 8385 EP 8391 DI 10.1021/jf501742h PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AN6WI UT WOS:000340738700020 PM 25050614 ER PT J AU Wang, YS Bradford, SA Simunek, J AF Wang, Yusong Bradford, Scott A. Simunek, Jiri TI Release of E-coli D21g with Transients in Water Content SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID SATURATED POROUS-MEDIA; COLLOID-FACILITATED TRANSPORT; CHEMICAL CONDITIONS; IONIC-STRENGTH; CRYPTOSPORIDIUM OOCYSTS; HYDRAULIC CONDUCTIVITY; UNSATURATED SAND; FLOW CONDITIONS; MOBILIZATION; RETENTION AB Transients in water content are well-known to mobilize microorganisms that are retained in the vadose zone. However, there is no consensus on the relative importance of drainage and imbibition events on microorganism release. To overcome this limitation, we have systematically studied the release of Escherichia coli D21g during cycles of drainage and imbibition under various solution chemistry and initial conditions. Results from these column studies revealed the influence of imbibition and drainage on D21g release. In particular, imbibition efficiently released cells from the air water interface (AWI) that were initially retained under steady-state unsaturated conditions by expansion of water films and destruction of the AWL Conversely, significant release and transport of cells during drainage only occurred below a critical water saturation (water film thickness). In this case, a fraction of the cells that were initially retained on the solid water interface (SWI) partitioned into the mobile aqueous phase and the SWI as the receding water film thickness decreased during drainage. The efficiency of cell release from the SWI during drainage was much less than for the AWI during imbibition. Cycles of drainage and imbibition removed cells from the SWI and the AWI, respectively. However, the peak concentration and amount of cells that were released increased with the number of retained cells and the amount of drainage and imbibition, and decreased with the number of drainage and imbibition cycles. Release of cells during drainage and imbibition was found to be more pronounced in the presence of a weak secondary minimum when the ionic strength (IS) was 5 mM NaCl. Increases in the solution IS decreased the influence of water transients on release, especially during drainage. Complete recovery of the retained cells could be achieved using both IS reduction and cycles of drainage and imbibition, even when the cells were retained under favorable attachment conditions. In general, cell release was more pronounced with transients in water content than transients in IS when the IS >= 5 mM. C1 [Wang, Yusong; Simunek, Jiri] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Bradford, Scott A.] ARS, US Salin Lab, USDA, Riverside, CA 92521 USA. RP Wang, YS (reprint author), Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. EM ywang032@ucr.edu FU 214 Manure and Byproduct Utilization Project of the USDA-ARS FX This research was supported by the 214 Manure and Byproduct Utilization Project of the USDA-ARS. Mention of trade names and company names in this manuscript does not imply any endorsement or preferential treatment by the USDA. We also acknowledge the help of Teresa Clapp in conducting these transport and release experiments. NR 73 TC 2 Z9 2 U1 5 U2 21 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 19 PY 2014 VL 48 IS 16 BP 9349 EP 9357 DI 10.1021/es501956k PG 9 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AN6JW UT WOS:000340701800050 PM 25040920 ER PT J AU DeLucia, EH Gomez-Casanovas, N Greenberg, JA Hudiburg, TW Kantola, IB Long, SP Miller, AD Ort, DR Parton, WJ AF DeLucia, Evan H. Gomez-Casanovas, Nuria Greenberg, Jonathan A. Hudiburg, Tara W. Kantola, Ilsa B. Long, Stephen P. Miller, Adam D. Ort, Donald R. Parton, William J. TI The Theoretical Limit to Plant Productivity SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID NET PRIMARY PRODUCTION; HUMAN APPROPRIATION; CONVERSION EFFICIENCY; ENERGY-CONVERSION; CROP PRODUCTION; SOLAR-ENERGY; WINTER-WHEAT; WATER-USE; EVAPOTRANSPIRATION; PHOTOSYNTHESIS AB Human population and economic growth are accelerating the demand for plant biomass to provide food, fuel, and fiber. The annual increment of biomass to meet these needs is quantified as net primary production (NPP). Here we show that an underlying assumption in some current models may lead to underestimates of the potential production from managed landscapes, particularly of bioenergy crops that have low nitrogen requirements. Using a simple light-use efficiency model and the theoretical maximum efficiency with which plant canopies convert solar radiation to biomass, we provide an upper-envelope NPP unconstrained by resource limitations. This theoretical maximum NPP approached 200 tC ha(-1) yr(-1) at point locations, roughly 2 orders of magnitude higher than most current managed or natural ecosystems. Recalculating the upper envelope estimate of NPP limited by available water reduced it by half or more in 91% of the land area globally. While the high conversion efficiencies observed in some extant plants indicate great potential to increase crop yields without changes to the basic mechanism of photosynthesis, particularly for crops with low nitrogen requirements, realizing such high yields will require improvements in water use efficiency. C1 [DeLucia, Evan H.; Long, Stephen P.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [DeLucia, Evan H.; Gomez-Casanovas, Nuria; Hudiburg, Tara W.; Kantola, Ilsa B.; Long, Stephen P.; Miller, Adam D.] Univ Illinois, Energy Biosci Inst, Urbana, IL 61801 USA. [DeLucia, Evan H.] Univ Illinois, Inst Sustainabil Energy & Environm, Urbana, IL 61801 USA. [Greenberg, Jonathan A.] Univ Illinois, Dept Geog & Geog Informat Sci, Urbana, IL 61801 USA. [Long, Stephen P.; Ort, Donald R.] Univ Illinois, Dept Crop Sci, Urbana, IL 61801 USA. [Ort, Donald R.] ARS, Photosynth Res Unit, USDA, Urbana, IL 61801 USA. [Parton, William J.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. RP DeLucia, EH (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM delucia@illinois.edu OI Greenberg, Jonathan/0000-0001-8435-9077 FU Energy Biosciences Institute, University of California at Berkeley, Berkeley, CA FX We gratefully acknowledge funding for this research from the Energy Biosciences Institute, University of California at Berkeley, Berkeley, CA. The functions describing the temperature dependence of epsilonc for C3 and C4 plants were provided by Gui-Lian Zhang and Xinguang Zhu, Partner Institute for Computational Biology Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. The authors greatly appreciate thoughtful discussions about this research with Steve W. Running and William Kolby Smith. NR 60 TC 10 Z9 10 U1 8 U2 54 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 19 PY 2014 VL 48 IS 16 BP 9471 EP 9477 DI 10.1021/es502348e PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AN6JW UT WOS:000340701800064 PM 25069060 ER PT J AU Stewart, SD Lorenz, GM Catchot, AL Gore, J Cook, D Skinner, J Mueller, TC Johnson, DR Zawislak, J Barber, J AF Stewart, Scott D. Lorenz, Gus M. Catchot, Angus L. Gore, Jeff Cook, Don Skinner, John Mueller, Thomas C. Johnson, Donald R. Zawislak, Jon Barber, Jonathan TI Potential Exposure of Pollinators to Neonicotinoid Insecticides from the Use of Insecticide Seed Treatments in the Mid-Southern United States SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID APIS-MELLIFERA L.; HONEY-BEES; LIQUID-CHROMATOGRAPHY; TREATED MAIZE; HYMENOPTERA; AGROCHEMICALS; CLOTHIANIDIN; THIAMETHOXAM; RESIDUES; BEHAVIOR AB Research was done during 2012 to evaluate the potential exposure of pollinators to neonicotinoid insecticides used as seed treatments on corn, cotton, and soybean. Samples were collected from small plot evaluations of seed treatments and from commercial fields in agricultural production areas in Arkansas, Mississippi, and Tennessee. In total, 560 samples were analyzed for concentrations of clothianidin, imidacloprid, thiamethoxam, and their metabolites. These induded pollen from corn and cotton, nectar from cotton, flowers from soybean, honey bees, Apis mellifera L., and pollen carried by foragers returning to hives, preplanting and in-season soil samples, and wild flowers adjacent to recently planted fields. Neonicotinoid insecticides were detected at a level of 1 ng/g or above in 23% of wild flower samples around recently planted fields, with an average detection level of about 10 ng/g. We detected neonicotinoid insecticides in the soil of production fields prior to planting at an average concentration of about 10 ng/g, and over 80% of the samples having some insecticide present. Only 5% of foraging honey bees tested positive for the presence of neonicotinoid insecticides, and there was only one trace detection (< 1 ng/g) in pollen being carried by those bees. Soybean flowers, cotton pollen, and cotton nectar contained little or no neonicotinoids resulting from insecticide seed treatments. Average levels of neonicotinoid insecticides in corn pollen ranged from less than 1 to 6 ng/g. The highest neonicotinoid concentrations were found in soil collected during early flowering from insecticide seed treatment trials. However, these levels were generally not well correlated with neonicotinoid concentrations in flowers, pollen, or nectar. Concentrations in flowering structures were well below defined levels of concern thought to cause acute mortality in honey bees. The potential implications of our findings are discussed. C1 [Stewart, Scott D.; Skinner, John; Mueller, Thomas C.] Univ Tennessee, Inst Agr, Knoxville, TN 37996 USA. [Lorenz, Gus M.; Johnson, Donald R.; Zawislak, Jon] Univ Arkansas, Div Agr, Lonoke, AR 72086 USA. [Catchot, Angus L.; Gore, Jeff; Cook, Don] Mississippi State Univ, Div Agr, Mississippi State, MS 39762 USA. [Barber, Jonathan] ARS, USDA, Sci & Technol Lab, Approval & Testing Div,Natl Sci Labs,Gastonia Lab, Gastonia, NC 28054 USA. RP Stewart, SD (reprint author), WTREC, 605 Airways Blvd, Jackson, TN 38301 USA. EM sdstewart@utk.edu FU National Cotton Council; Cotton Incorporated; Arkansas Soybean Promotion Board; Mississippi Soybean Promotion Board; Tennessee Soybean Promotion Board; Mississippi Corn Promotion Board FX We appreciate the funding provided to this project provided by the National Cotton Council and Cotton Incorporated, the Arkansas, Mississippi, and Tennessee Soybean Promotion Boards, and the Mississippi Corn Promotion Board. NR 35 TC 25 Z9 26 U1 12 U2 132 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 19 PY 2014 VL 48 IS 16 BP 9762 EP 9769 DI 10.1021/es501657w PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AN6JW UT WOS:000340701800098 PM 25010122 ER PT J AU Chen, JX Colombo, SJ Ter-Mikaelian, MT Heath, LS AF Chen, Jiaxin Colombo, Stephen J. Ter-Mikaelian, Michael T. Heath, Linda S. TI Carbon Profile of the Managed Forest Sector in Canada in the 20th Century: Sink or Source? SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article ID BOREAL FOREST; FIRE FREQUENCY; WOOD PRODUCTS; BALANCE; SUBSTITUTION; SUPPRESSION; MITIGATION; ONTARIO; IMPACTS; GROWTH AB Canada contains 10% of global forests and has been one of the world's largest harvested wood products (HWP) producers. Therefore, Canada's managed forest sector, the managed forest area and HWP, has the potential to significantly increase or reduce atmospheric greenhouse gases. Using the most comprehensive carbon balance analysis to date, this study shows Canada's managed forest area and resulting HWP were a sink of 7510 and 849 teragrams carbon (TgC), respectively, in the period 1901-2010, exceeding Canada's fossil fuel-based emissions over this period (7333 TgC). If Canadian HWP were not produced and used for residential construction, and instead more energy intensive materials were used, there would have been an additional 790 TgC fossil fuel-based emissions. Because the forest carbon increases in the 20th century were mainly due to younger growing forests that resulted from disturbances in the 19th century, and future increases in forest carbon stocks appear uncertain, in coming decades most of the mitigation contribution from Canadian forests will likely accrue from wood substitution that reduces fossil fuel-based emissions and stores carbon, so long as those forests are managed sustainably. C1 [Chen, Jiaxin; Colombo, Stephen J.; Ter-Mikaelian, Michael T.] Minist Nat Resources & Forestry, Ontario Forest Res Inst, Sault Ste Marie, ON P6A 2E5, Canada. [Heath, Linda S.] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. RP Chen, JX (reprint author), Minist Nat Resources & Forestry, Ontario Forest Res Inst, 1235 Queen St East, Sault Ste Marie, ON P6A 2E5, Canada. EM jiaxin.chen@ontario.ca NR 45 TC 2 Z9 2 U1 4 U2 24 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD AUG 19 PY 2014 VL 48 IS 16 BP 9859 EP 9866 DI 10.1021/es5005957 PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AN6JW UT WOS:000340701800110 PM 25075978 ER PT J AU Brockmeier, SL Register, KB Kuehn, JS Nicholson, TL Loving, CL Bayles, DO Shore, SM Phillips, GJ AF Brockmeier, Susan L. Register, Karen B. Kuehn, Joanna S. Nicholson, Tracy L. Loving, Crystal L. Bayles, Darrell O. Shore, Sarah M. Phillips, Gregory J. TI Virulence and Draft Genome Sequence Overview of Multiple Strains of the Swine Pathogen Haemophilus parasuis SO PLOS ONE LA English DT Article ID SERUM-RESISTANCE; PIGS; INFECTION; PROTEINS; SEROVARS; DISEASE; IDENTIFICATION; COLONIZATION; PROTECTION; ALIGNMENT AB Haemophilus parasuis is the cause of Glasser's disease in swine, which is characterized by systemic infection resulting in polyserositis, meningitis, and arthritis. Investigation of this animal disease is complicated by the enormous differences in the severity of disease caused by H. parasuis strains, ranging from lethal systemic disease to subclinical carriage. To identify differences in genotype that could account for virulence phenotypes, we established the virulence of, and performed whole genome sequence analysis on, 11 H. parasuis strains. Virulence was assessed by evaluating morbidity and mortality following intranasal challenge of Caesarean-derived, colostrum-deprived (CDCD) pigs. Genomic DNA from strains Nagasaki (serotype 5), 12939 (serotype 1), SW140 (serotype 2), 29755 (serotype 5), MN-H (serotype 13), 84-15995 (serotype 15), SW114 (serotype 3), H465 (serotype 11), D74 (serotype 9), and 174 (serotype 7) was used to generate Illumina paired-end libraries for genomic sequencing and de novo assembly. H. parasuis strains Nagasaki, 12939, SH0165 (serotype 5), SW140, 29755, and MN-H exhibited a high level of virulence. Despite minor differences in expression of disease among these groups, all pigs challenged with these strains developed clinical signs consistent with Glasser's disease between 1-7 days post-challenge. H. parasuis strains 84-15995 and SW114 were moderately virulent, in that approximately half of the pigs infected with each developed Glasser's disease. H. parasuis strains H465, D74, and 174 were minimally virulent or avirulent in the CDCD pig model. Comparative genomic analysis among strains identified several noteworthy differences in coding regions. These coding regions include predicted outer membrane, metabolism, and pilin or adhesin related genes, some of which likely contributed to the differences in virulence and systemic disease observed following challenge. These data will be useful for identifying H. parasuis virulence factors and vaccine targets. C1 [Brockmeier, Susan L.; Nicholson, Tracy L.; Loving, Crystal L.; Shore, Sarah M.] ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. [Register, Karen B.] ARS, Ruminant Dis & Immunol Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. [Kuehn, Joanna S.; Phillips, Gregory J.] Iowa State Univ, Dept Vet Microbiol & Prevent Med, Coll Vet Med, Ames, IA USA. [Bayles, Darrell O.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA USA. RP Brockmeier, SL (reprint author), ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. EM susan.brockmeier@ars.usda.gov FU Iowa State Healthy Livestock Initiative; USDA Agricultural Research Service FX Boehringer Ingelheim Vetmedica, Inc. donated pigs for the swine study and paid partial post-doctoral salary of J. S. Kuehn. Additional funding came from the Iowa State Healthy Livestock Initiative and the USDA Agricultural Research Service. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 6 Z9 6 U1 3 U2 13 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 19 PY 2014 VL 9 IS 8 AR e103787 DI 10.1371/journal.pone.0103787 PG 13 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN6XM UT WOS:000340742100014 PM 25137096 ER PT J AU Tang, YJ Kim, H Singh, AK Aroonnual, A Bae, E Rajwa, B Fratamico, PM Bhunia, AK AF Tang, Yanjie Kim, Huisung Singh, Atul K. Aroonnual, Amornrat Bae, Euiwon Rajwa, Bartek Fratamico, Pina M. Bhunia, Arun K. TI Light Scattering Sensor for Direct Identification of Colonies of Escherichia coli Serogroups O26, O45, O103, O111, O121, O145 and O157 SO PLOS ONE LA English DT Article ID SORBITOL-MACCONKEY MEDIUM; GROUND-BEEF; GENE-CLUSTER; ANTIGEN 43; O-ANTIGEN; FOOD; MEDIA; AGAR; PCR; INFECTIONS AB Background: Shiga-toxin producing Escherichia coli (STEC) have emerged as important foodborne pathogens, among which seven serogroups (O26, O45, O103, O111, O121, O145, O157) are most frequently implicated in human infection. The aim was to determine if a light scattering sensor can be used to rapidly identify the colonies of STEC serogroups on selective agar plates. Methodology/Principal Findings: Initially, a total of 37 STEC strains representing seven serovars were grown on four different selective agar media, including sorbitol MacConkey (SMAC), Rainbow Agar O157, BBL CHROMagarO157, and R&F E. coli O157:H7, as well as nonselective Brain Heart Infusion agar. The colonies were scanned by an automated light scattering sensor, known as BARDOT (BActerial Rapid Detection using Optical scattering Technology), to acquire scatter patterns of STEC serogroups, and the scatter patterns were analyzed using an image classifier. Among all of the selective media tested, both SMAC and Rainbow provided the best differentiation results allowing multi-class classification of all serovars with an average accuracy of more than 90% after 10-12 h of growth, even though the colony appearance was indistinguishable at that early stage of growth. SMAC was chosen for exhaustive scatter image library development, and 36 additional strains of O157:H7 and 11 non-O157 serovars were examined, with each serogroup producing unique differential scatter patterns. Colony scatter images were also tested with samples derived from pure and mixed cultures, as well as experimentally inoculated food samples. BARDOT accurately detected O157 and O26 serovars from a mixed culture and also from inoculated lettuce and ground beef (10-h broth enrichment +12-h on-plate incubation) in the presence of natural background microbiota in less than 24 h. Conclusions: BARDOT could potentially be used as a screening tool during isolation of the most important STEC serovars on selective agar plates from food samples in less than 24 h. C1 [Tang, Yanjie; Singh, Atul K.; Aroonnual, Amornrat; Bhunia, Arun K.] Purdue Univ, Dept Food Sci, Mol Food Microbiol Lab, W Lafayette, IN 47907 USA. [Kim, Huisung; Bae, Euiwon] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA. [Rajwa, Bartek] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA. [Fratamico, Pina M.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA USA. [Bhunia, Arun K.] Purdue Univ, Dept Comparat Pathobiol, W Lafayette, IN 47907 USA. RP Bhunia, AK (reprint author), Purdue Univ, Dept Food Sci, Mol Food Microbiol Lab, Smith Hall, W Lafayette, IN 47907 USA. EM bhunia@purdue.edu RI Rajwa, Bartek/B-3169-2009 OI Rajwa, Bartek/0000-0001-7540-8236 FU Agriculture Research Service of the United States Department of Agriculture [1935-42000-072- 02G]; Center for Food Safety Engineering (CFSE) at Purdue University FX The project was supported by funds from the Agriculture Research Service of the United States Department of Agriculture project number 1935-42000-072- 02G, and the Center for Food Safety Engineering (CFSE) at Purdue University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 60 TC 13 Z9 14 U1 2 U2 28 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 19 PY 2014 VL 9 IS 8 AR e105272 DI 10.1371/journal.pone.0105272 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN6XM UT WOS:000340742100082 PM 25136836 ER PT J AU Liang, ZC Cheng, LL Zhong, GY Liu, RH AF Liang, Zhenchang Cheng, Lailiang Zhong, Gan-Yuan Liu, Rui Hai TI Antioxidant and Antiproliferative Activities of Twenty-Four Vitis vinifera Grapes SO PLOS ONE LA English DT Article ID ANTI PROLIFERATIVE ACTIVITIES; WOODCHUCK HEPATITIS-VIRUS; KAPPA-B ACTIVATION; CANCER MCF-7 CELLS; COMMON FRUITS; RED WINES; POLYPHENOLS; ASSAY; PHYTOCHEMICALS; PROFILES AB Grapes are rich in phytochemicals with many proven health benefits. Phenolic profiles, antioxidant and antiproliferative activities of twenty-four selected Vitis vinifera grape cultivars were investigated in this study. Large ranges of variation were found in these cultivars for the contents of total phenolics (95.3 to 686.5 mg/100 g) and flavonoids (94.7 to 1055 mg/100 g) and antioxidant activities (oxygen radical absorbance capacity 378.7 to 3386.0 mg of Trolox equivalents/100 g and peroxylradical scavenging capacity14.2 to 557 mg of vitamin C equivalents/100 g), cellular antioxidant activities (3.9 to 139.9 mu mol of quercetin equivalents/100 g without PBS wash and 1.4 to 95.8 mu mol of quercetin equivalents/100 g with PBS wash) and antiproliferative activities (25 to 82% at the concentrations of 100 mg/mL extracts). The total antioxidant activities were significantly correlated with the total phenolics and flavonoids. However, no significant correlations were found between antiproliferative activities and total phenolics or total flavonoids content. Wine grapes and color grapes showed much higher levels of phytochemicals and antioxidant activities than table grapes and green/yellow grapes. Several germplasm accessions with much high contents of phenolics and flavonoids, and total antioxidant activity were identified. These germplasm can be valuable sources of genes for breeding grape cultivars with better nutritional qualities of wine and table grapes in the future. C1 [Liang, Zhenchang] Chinese Acad Sci, Inst Bot, Beijing, Peoples R China. [Liang, Zhenchang; Liu, Rui Hai] Cornell Univ, Dept Food Sci, Ithaca, NY 14853 USA. [Cheng, Lailiang] Cornell Univ, Dept Hort, Ithaca, NY USA. [Zhong, Gan-Yuan] USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. RP Zhong, GY (reprint author), USDA ARS, Grape Genet Res Unit, Geneva, NY 14456 USA. EM ganyuan.zhong@ars.usda.gov; rl23@cornell.edu RI Liu, Rui Hai/C-2865-2008 FU USDA-ARS; Cornell University; United States Department of Agriculture-Agricultural Research Service [1910-21220-004-00D] FX The authors are grateful to Mr. Bernard Prins of the USDA-ARS National Clonal Germplasm Repository for Fruit and Nut Crops in Davis, California and Dr. Christopher Owens of the USDA-ARS Grape Genetics Research Unit in Geneva, New York for providing V. vinifera berry samples. This work was supported by a Special Cooperative Agreement between USDA-ARS and Cornell University. USDA-ARS is an equal opportunity provider and employer. This project was supported by the United States Department of Agriculture-Agricultural Research Service Project 1910-21220-004-00D through a Specific Cooperative Agreement with Cornell University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 39 TC 12 Z9 12 U1 8 U2 67 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 18 PY 2014 VL 9 IS 8 AR e105146 DI 10.1371/journal.pone.0105146 PG 10 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO4JF UT WOS:000341302700077 PM 25133401 ER PT J AU Palmer, NA Saathoff, AJ Tobias, CM Twigg, P Xia, YN Vogel, KP Madhavan, S Sattler, SE Sarath, G AF Palmer, Nathan A. Saathoff, Aaron J. Tobias, Christian M. Twigg, Paul Xia, Yuannan Vogel, Kenneth P. Madhavan, Soundararajan Sattler, Scott E. Sarath, Gautam TI Contrasting Metabolism in Perenniating Structures of Upland and Lowland Switchgrass Plants Late in the Growing Season SO PLOS ONE LA English DT Article ID WRKY TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; LIGNIN BIOSYNTHESIS; STRESS TOLERANCE; SENESCENCE; PROTEINS; DORMANCY; GRASSES; PEROXIDASES; ANTIOXIDANT AB Background: Switchgrass (Panicum virgatum L.) is being developed as a bioenergy crop for many temperate regions of the world. One way to increase biomass yields is to move southern adapted lowland cultivars to more northern latitudes. However, many southerly adapted switchgrass germplasm can suffer significant winter kill in northerly climes. Materials and Methods: Here, we have applied next-generation sequencing in combination with biochemical analyses to query the metabolism of crowns and rhizomes obtained from two contrasting switchgrass cultivars. Crowns and rhizomes from field-grown lowland (cv Kanlow) and upland (cv Summer) switchgrass cultivars were collected from three randomly selected post-flowering plants. Summer plants were senescing, whereas Kanlow plants were not at this harvest date. Results: Principal component analysis (PCA) differentiated between both the Summer and Kanlow transcriptomes and metabolomes. Significant differences in transcript abundances were detected for 8,050 genes, including transcription factors such as WRKYs and those associated with phenylpropanoid biosynthesis. Gene-set enrichment analyses showed that a number of pathways were differentially up-regulated in the two populations. For both populations, protein levels and enzyme activities agreed well with transcript abundances for genes involved in the phenylpropanoid pathway that were up-regulated in Kanlow crowns and rhizomes. The combination of these datasets suggests that dormancy-related mechanisms had been triggered in the crowns and rhizomes of the Summer plants, whereas the crowns and rhizomes of Kanlow plants had yet to enter dormancy. Conclusions: Delayed establishment of dormancy at more northerly latitudes could be one factor that reduces winter-survival in the high-yielding Kanlow plants. Understanding the cellular signatures that accompany the transition to dormancy can be used in the future to select plants with improved winter hardiness. C1 [Palmer, Nathan A.; Saathoff, Aaron J.; Sattler, Scott E.; Sarath, Gautam] Univ Nebraska, USDA ARS, Forage & Bioenergy Res Unit, Lincoln, NE 68588 USA. [Palmer, Nathan A.; Saathoff, Aaron J.; Sattler, Scott E.; Sarath, Gautam] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Tobias, Christian M.] USDA ARS, Western Reg Res Ctr, Genom & Gene Discovery Res Unit, Albany, CA 94710 USA. [Twigg, Paul] Univ Nebraska, Dept Biol, Kearney, NE USA. [Xia, Yuannan] Univ Nebraska, Ctr Biotechnol, Lincoln, NE USA. [Vogel, Kenneth P.] USDA ARS, Lincoln, NE 68583 USA. [Madhavan, Soundararajan] Univ Nebraska, Dept Biochem, Lincoln, NE 68583 USA. RP Sarath, G (reprint author), Univ Nebraska, USDA ARS, Forage & Bioenergy Res Unit, Lincoln, NE 68588 USA. EM Gautam.Sarath@ars.usda.gov FU Office of Science (BER), U. S. Department of Energy [DE-AI02-09ER64829]; USDA-ARS CRIS [5440-21000-030-00D] FX This work was supported by the Office of Science (BER), U. S. Department of Energy Grant Number DE-AI02-09ER64829, and by the USDA-ARS CRIS project 5440-21000-030-00D. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 10 Z9 10 U1 1 U2 21 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 18 PY 2014 VL 9 IS 8 AR e105138 DI 10.1371/journal.pone.0105138 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO4JF UT WOS:000341302700076 PM 25133804 ER PT J AU Stauffer, KE Haven, JS Crews, JE AF Stauffer, Kendra E. Haven, John S. Crews, John E. TI Emergency preparedness in Florida SO JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION LA English DT Letter C1 [Stauffer, Kendra E.] USDA, Vet Serv, APHIS, Gainesville, FL 32608 USA. [Haven, John S.] Univ Florida, Coll Vet Med, Gainesville, FL USA. [Crews, John E.] Florida Dept Agr & Consumer Serv, Tallahassee, FL USA. RP Stauffer, KE (reprint author), USDA, Vet Serv, APHIS, Gainesville, FL 32608 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER VETERINARY MEDICAL ASSOC PI SCHAUMBURG PA 1931 N MEACHAM RD SUITE 100, SCHAUMBURG, IL 60173-4360 USA SN 0003-1488 EI 1943-569X J9 JAVMA-J AM VET MED A JI JAVMA-J. Am. Vet. Med. Assoc. PD AUG 15 PY 2014 VL 245 IS 4 BP 373 EP 373 PG 1 WC Veterinary Sciences SC Veterinary Sciences GA CA6CZ UT WOS:000348997400011 PM 25211784 ER PT J AU Potter, TL Bosch, DD Strickland, TC AF Potter, Thomas L. Bosch, David D. Strickland, Timothy C. TI Comparative assessment of herbicide and fungicide runoff risk: A case study for peanut production in the Southern Atlantic Coastal Plain (USA) SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Conservation tillage; Erosion; Risk assessment; Peanut; Tebuconazole; Metolachlor ID PESTICIDE RUNOFF; SURFACE WATERS; CONSERVATION TILLAGE; RAINFALL CONDITIONS; VINEYARD CATCHMENT; LOWLAND STREAMS; FATE; IMPACT; FIELD; SOIL AB Peanut (Arachis hypogaea) is produced intensively in the southern Atlantic Coastal Plain of the eastern USA. To effectively protect the region's water quality data are needed which quantify runoff of pesticides used to protect these crops. Fungicides are used intensively yet there is little published data which describe their potential for loss in surface runoff. This study compared runoff of a fungicide, tebuconazole (alpha-[2-(4-chlorophenyl)ethyl]-alpha-(1,1-dimethylethyl)-1H-1,2,4-triazole-1-ethanol), and an herbicide, metolachlor (2-chloro-N-(2-ethyl-6-methylphenyl)- N-(2-methoxy-1-methylethyl) acetamide) from 0.2 ha fields in strip (ST), a commonly used conservation-tillage practice, and conventional tillage (CT) near Tifton, GA (USA). Following their first application, metolachlor and tebuconazole were detected at high frequency in runoff. Concentrations and their annual losses increased with application frequency and runoff event timing and frequency with respect to applications, and when fields were positioned at the top of the slope and CT was practiced. Runoff one day after treatment (DAT) contributed to high tebuconazole runoff loss, up to 9.8% of the amount applied on an annual basis. In all cases, metolachlor loss was more than 10 times less even though total application was 45% higher. This was linked to the fact that the one metolachlor application to each crop was in May, one of the region's driest months. In sum, studies showed that fungicide runoff rates may be relatively high and emphasize the need to focus on these products in future studies on peanut and other crops. The study also showed that peanut farmers should be encouraged to use conservation tillage practices like ST which can substantially reduce pesticide runoff. Published by Elsevier B.V. C1 [Potter, Thomas L.; Bosch, David D.; Strickland, Timothy C.] USDA ARS, SE Watershed Res Lab, Tifton, GA 31793 USA. RP Potter, TL (reprint author), USDA ARS, SE Watershed Res Lab, POB 748, Tifton, GA 31793 USA. EM tom.potter@ars.usda.gov FU Georgia Cotton Commission; USDA-Agricultural Research Service; University of Georgia Coastal Plain Experiment Station; U.S.-Israel Binational Agricultural Research and Development Fund FX The Georgia Cotton Commission, the USDA-Agricultural Research Service, the University of Georgia Coastal Plain Experiment Station, and the U.S.-Israel Binational Agricultural Research and Development Fund supported this work. USDA employees Herman Henry (retired), Ricky Fletcher, Margie Whittle, Sally Bellflower, Lorine Lewis, Ernest Harris, Laura Marshall (former employee) and numerous undergraduate students provided expert assistance. NR 50 TC 6 Z9 6 U1 0 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2014 VL 490 BP 1 EP 10 DI 10.1016/j.scitotenv.2014.04.034 PG 10 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY0NT UT WOS:000347293800001 PM 24836324 ER PT J AU Ma, JC Ibekwe, AM Crowley, DE Yang, CH AF Ma, Jincai Ibekwe, A. Mark Crowley, David E. Yang, Ching-Hong TI Persistence of Escherichia coli O157 and non-O157 strains in agricultural soils SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Escherichia coli O157 and non-O157; Preharvest; Persistence; Fresh produce; Soil; Contamination ID MANURE-AMENDED SOIL; BROMINATED FLAME RETARDANTS; HEMOLYTIC-UREMIC SYNDROME; SHIGA TOXIN; PATHOGENIC BACTERIA; SURVIVAL; TRANSMISSION; INFECTIONS; LETTUCE; IDENTIFICATION AB Shiga toxin producing Escherichia coli O157 and non-O157 serogroups are known to cause serious diseases in human. However, research on the persistence of E. coli non-O157 serogroups in preharvest environment is limited. In the current study, we compared the survival behavior of E. coli O157 to that of non-O157 E. coli strains in agricultural soils collected from three major fresh produce growing areas of California (CA) and Arizona (AZ). Results showed that the nonpathogenic E. coli O157:H7 4554 survived longer than the pathogenic E. coli O157: H7 EDL933 in Imperial Valley CA and Yuma AZ, but not in soils from the Salinas area. However, E. coli O157: NM was found to persist significantly longer than E. coli O157: H7 EDL933 in all soil tested from the three regions. Furthermore, two non-O157 (E. coli O26:H21 and E. coli O103:H2) survived significantly longer than E. coli O157: H7 EDL933 in all soils tested. Pearson correlation analysis showed that survival of the E. coli strains was affected by different environmental factors. Our data suggest that survival of E. coli O157 and non-O157 may be strain and soil specific, and therefore, care must be taken in data interpretation with respect to survival of this pathogen in different soils. Published by Elsevier B.V. C1 [Ma, Jincai; Ibekwe, A. Mark] ARS, USDA, US Salin Lab, Riverside, CA 92507 USA. [Ma, Jincai; Crowley, David E.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Yang, Ching-Hong] Univ Wisconsin, Dept Biol Sci, Milwaukee, WI 53211 USA. RP Ibekwe, AM (reprint author), ARS, USDA, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM Mark.Ibekwe@ars.usda.gov RI Crowley, David/C-1216-2014; Ma, Jincai/D-1290-2013 OI Crowley, David/0000-0002-1805-8599; Ma, Jincai/0000-0002-0792-0251 FU CSREES NIFA [2008-35201-18709]; 214 Manure and Byproduct Utilization Project of the USDA-ARS FX This research was supported by the CSREES NIFA Agreement No., 2008-35201-18709 and the 214 Manure and Byproduct Utilization Project of the USDA-ARS. We thank Drs Jorge Fonseca of the University of Arizona Yuma, Mark Trent, UC-Davis, Imperial Agricultural Experiment Station, and James McCreight of USDA-ARS Salinas CA for providing soil samples for this study. We also thank Damon Baptista for the technical help. Mention of trademark or propriety products in this manuscript does not constitute a guarantee or warranty of the property by the USDA and does not imply its approval to the exclusion of other products that may also be suitable. NR 52 TC 9 Z9 9 U1 3 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2014 VL 490 BP 822 EP 829 DI 10.1016/j.scitotenv.2014.05.069 PG 8 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY0NT UT WOS:000347293800087 PM 24907617 ER PT J AU Corsi, SR Borchardt, MA Spencer, SK Hughes, PE Baldwin, AK AF Corsi, S. R. Borchardt, M. A. Spencer, S. K. Hughes, P. E. Baldwin, A. K. TI Human and bovine viruses in the Milwaukee River watershed: Hydrologically relevant representation and relations with environmental variables SO SCIENCE OF THE TOTAL ENVIRONMENT LA English DT Article DE Human viruses; Watershed; Hydrologic event; Automated sampling; Seasonality ID HUMAN ENTERIC VIRUSES; ACUTE GASTROINTESTINAL ILLNESS; DRINKING-WATER; AQUATIC ENVIRONMENTS; FECAL CONTAMINATION; HUMAN ADENOVIRUSES; SOURCE-TRACKING; COASTAL WATERS; SEPTIC SYSTEM; HEALTH-RISKS AB To examine the occurrence, hydrologic variability, and seasonal variability of human and bovine viruses in surface water, three stream locations were monitored in the Milwaukee River watershed in Wisconsin, USA, from February 2007 through June 2008. Monitoring sites included an urban subwatershed, a rural subwatershed, and the Milwaukee River at the mouth. To collect samples that characterize variability throughout changing hydrologic periods, a process control system was developed for unattended, large-volume (56-2800 L) filtration over extended durations. This system provided flow-weighted mean concentrations during runoff and extended (24-h) low-flow periods. Human viruses and bovine viruses were detected by real-time qPCR in 49% and 41% of samples (n = 63), respectively. All human viruses analyzed were detected at least once including adenovirus (40% of samples), GI norovirus (10%), enterovirus (8%), rotavirus (6%), GII norovirus (1.6%) and hepatitis A virus (1.6%). Three of seven bovine viruses analyzed were detected including bovine polyomavirus (32%), bovine rotavirus (19%), and bovine viral diarrhea virus type 1 (5%). Human viruses were present in 63% of runoff samples resulting from precipitation and snowmelt, and 20% of low-flow samples. Maximum human virus concentrations exceeded 300 genomic copies/L. Bovine viruses were present in 46% of runoff samples resulting from precipitation and snowmelt and 14% of low-flow samples. The maximum bovine virus concentration was 11 genomic copies/L. Statistical modeling indicated that stream flow, precipitation, and season explained the variability of human viruses in the watershed, and hydrologic condition (runoff event or low-flow) and season explained the variability of the sum of human and bovine viruses; however, no model was identified that could explain the variability of bovine viruses alone. Understanding the factors that affect virus fate and transport in rivers will aid watershed management for minimizing human exposure and disease transmission. (C) 2014 Published by Elsevier B.V. C1 [Corsi, S. R.; Hughes, P. E.; Baldwin, A. K.] US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI 53562 USA. [Borchardt, M. A.; Spencer, S. K.] ARS, USDA, Marshfield, WI 54449 USA. RP Corsi, SR (reprint author), US Geol Survey, Wisconsin Water Sci Ctr, Middleton, WI 53562 USA. EM srcorsi@usgs.gov OI Baldwin, Austin/0000-0002-6027-3823 FU Milwaukee Metropolitan Sewerage District [11E4WI24910031]; U.S. Geological Survey FX Support for this research was provided by the Milwaukee Metropolitan Sewerage District (11E4WI24910031) and the U.S. Geological Survey. Thanks to Christopher Magruder from Milwaukee Metropolitan Sewerage District and Michelle Lutz and Jordan Gonnering in the U.S. Geological Survey, Cooperative Water Program for their contributions. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 73 TC 7 Z9 7 U1 1 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0048-9697 EI 1879-1026 J9 SCI TOTAL ENVIRON JI Sci. Total Environ. PD AUG 15 PY 2014 VL 490 BP 849 EP 860 DI 10.1016/j.scitotenv.2014.05.072 PG 12 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY0NT UT WOS:000347293800090 PM 24908645 ER PT J AU Xu, LY Cole, JB Bickhart, DM Hou, YL Song, JZ VanRaden, PM Sonstegard, TS Van Tassell, CP Liu, GE AF Xu, Lingyang Cole, John B. Bickhart, Derek M. Hou, Yali Song, Jiuzhou VanRaden, Paul M. Sonstegard, Tad S. Van Tassell, Curtis P. Liu, George E. TI Genome wide CNV analysis reveals additional variants associated with milk production traits in Holsteins SO BMC GENOMICS LA English DT Article DE Copy number variation (CNV); dPTA; Association; Milk production traits ID COPY-NUMBER VARIATION; SOMATIC-CELL SCORE; GROWTH-HORMONE RECEPTOR; AYRSHIRE DAIRY-CATTLE; FRIESIAN CATTLE; MISSENSE MUTATION; YIELD; GENE; LOCI; QTL AB Background: Milk production is an economically important sector of global agriculture. Much attention has been paid to the identification of quantitative trait loci (QTL) associated with milk, fat, and protein yield and the genetic and molecular mechanisms underlying them. Copy number variation (CNV) is an emerging class of variants which may be associated with complex traits. Results: In this study, we performed a genome-wide association between CNVs and milk production traits in 26,362 Holstein bulls and cows. A total of 99 candidate CNVs were identified using Illumina BovineSNP50 array data, and association tests for each production trait were performed using a linear regression analysis with PCA correlation. A total of 34 CNVs on 22 chromosomes were significantly associated with at least one milk production trait after false discovery rate (FDR) correction. Some of those CNVs were located within or near known QTL for milk production traits. We further investigated the relationship between associated CNVs with neighboring SNPs. For all 82 combinations of traits and CNVs (less than 400 kb in length), we found 17 cases where CNVs directly overlapped with tag SNPs and 40 cases where CNVs were adjacent to tag SNPs. In 5 cases, CNVs located were in strong linkage disequilibrium with tag SNPs, either within or adjacent to the same haplotype block. There were an additional 20 cases where CNVs did not have a significant association with SNPs, suggesting that the effects of those CNVs were probably not captured by tag SNPs. Conclusion: We conclude that combining CNV with SNP analyses reveals more genetic variations underlying milk production traits than those revealed by SNPs alone. C1 [Xu, Lingyang; Cole, John B.; Bickhart, Derek M.; VanRaden, Paul M.; Sonstegard, Tad S.; Van Tassell, Curtis P.; Liu, George E.] USDA ARS, Beltsville Agr Res Ctr, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. [Xu, Lingyang; Song, Jiuzhou] Univ Maryland, Dept Anim & Avian Sci, College Pk, MD 20742 USA. [Hou, Yali] Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China. RP Liu, GE (reprint author), USDA ARS, Beltsville Agr Res Ctr, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. EM George.Liu@ars.usda.gov RI Cole, John/J-8571-2014; OI Cole, John/0000-0003-1242-4401; Bickhart, Derek/0000-0003-2223-9285 FU AFRI from USDA NIFA [2011-67015-30183] FX We thank Reuben Anderson and Alexandre Dimitriv for technical assistance. This work was supported in part by AFRI grant No. 2011-67015-30183 from USDA NIFA. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 59 TC 17 Z9 18 U1 2 U2 15 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 15 PY 2014 VL 15 AR 683 DI 10.1186/1471-2164-15-683 PG 10 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AO0XP UT WOS:000341036500002 PM 25128478 ER PT J AU Humphrey, HM Orloski, KA Olea-Popelka, FJ AF Humphrey, Heather M. Orloski, Kathleen A. Olea-Popelka, Francisco J. TI Bovine tuberculosis slaughter surveillance in the United States 2001-2010: assessment of its traceback investigation function SO BMC VETERINARY RESEARCH LA English DT Article DE Bovine tuberculosis; Cattle; Slaughter surveillance; Traceback ID CATTLE; ERADICATION; EFFICIENCY; DISCLOSURE AB Background: The success of tracing cattle to the herd of origin after the detection and confirmation of bovine tuberculosis (TB) lesions in cattle at slaughter is a critical component of the national bovine TB eradication program in the United States (U.S.). The aims of this study were to 1) quantify the number of bovine TB cases identified at slaughter that were successfully traced to their herd of origin in the U.S. during 2001-2010, 2) quantify the number of successful traceback investigations that found additional TB infected animals in the herd of origin or epidemiologically linked herds, and 3) describe the forms of animal identification present on domestic bovine TB cases and their association with traceback success. Results: We analyzed 2001-2010 data in which 371 granulomatous lesions were confirmed as bovine TB. From these 114 bovine TB cases, 78 adults (i.e. sexually intact bovines greater than two years of age), and 36 fed (i.e. less than or equal to two years of age) were classified as domestic cattle (U.S. originated). Of these adults and fed cases, 83% and 13% were successfully traced, respectively. Of these traceback investigations, 70% of adult cases and 50% of fed cases identified additional bovine TB infected animals in the herd of origin or an epidemiologically linked herd. We found that the presence of various forms of animal identification on domestic bovine TB cases at slaughter may facilitate successful traceback investigations; however, they do not guarantee it. Conclusions: These results provide valuable information with regard to epidemiological traceback investigations and serve as a baseline to aid U.S. officials when assessing the impact of newly implemented strategies as part of the national bovine TB eradication in the U.S. C1 [Humphrey, Heather M.; Olea-Popelka, Francisco J.] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Clin Sci, Ft Collins, CO 80523 USA. [Orloski, Kathleen A.] USDA, APHIS, Ft Collins, CO 80523 USA. [Olea-Popelka, Francisco J.] Colorado State Univ, Coll Vet Med & Biomed Sci, MRL, Ft Collins, CO 80523 USA. RP Olea-Popelka, FJ (reprint author), Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Clin Sci, Ft Collins, CO 80523 USA. EM folea@colostate.edu FU Colorado State University (CSU); College of Veterinary Medicine and Biomedical Sciences; College Research Council; Applied Veterinary Epidemiology (AVE) research group at CSU FX We would like to thank the USDA/APHIS/VS for providing the bovine tuberculosis slaughter surveillance data set for 2001-2010 and the respective case files and the USDA/APHIS/VS National Veterinary Services Laboratory for laboratory expertise in confirming bovine TB infection. We would like to extend a special recognition to Dr. Robert Meyer for maintaining the bovine TB case files and for creating an electronic recording system. Furthermore, we would like to thank the Colorado State University (CSU), College of Veterinary Medicine and Biomedical Sciences, College Research Council and the Applied Veterinary Epidemiology (AVE) research group at CSU for providing funding to support this research project. NR 33 TC 1 Z9 1 U1 1 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD AUG 15 PY 2014 VL 10 AR 182 DI 10.1186/s12917-014-0182-y PG 10 WC Veterinary Sciences SC Veterinary Sciences GA AN9DX UT WOS:000340907300001 PM 25123050 ER PT J AU Alba, C Bowers, MD Blumenthal, D Hufbauer, RA AF Alba, Christina Bowers, M. Deane Blumenthal, Dana Hufbauer, Ruth A. TI Chemical and Mechanical Defenses Vary among Maternal Lines and Leaf Ages in Verbascum thapsus L. (Scrophulariaceae) and Reduce Palatability to a Generalist Insect SO PLOS ONE LA English DT Article ID JUNONIA-COENIA NYMPHALIDAE; BIOLOGICAL-CONTROL AGENT; IRIDOID GLYCOSIDES; NATURAL ENEMIES; PLANT DEFENSE; ARABIDOPSIS-THALIANA; HERBIVOROUS INSECTS; TRICHOME DENSITY; DATURA-WRIGHTII; COMMON MULLEIN AB Intra-specific variation in host-plant quality affects herbivore foraging decisions and, in turn, herbivore foraging decisions mediate plant fitness. In particular, variation in defenses against herbivores, both among and within plants, shapes herbivore behavior. If variation in defenses is genetically based, it can respond to natural selection by herbivores. We quantified intra-specific variation in iridoid glycosides, trichome length, and leaf strength in common mullein (Verbascum thapsus L, Scrophulariaceae) among maternal lines within a population and among leaves within plants, and related this variation to feeding preferences of a generalist herbivore, Trichopulsia ni Hubner. We found significant variation in all three defenses among maternal lines, with T. ni preferring plants with lower investment in chemical, but not mechanical, defense. Within plants, old leaves had lower levels of all defenses than young leaves, and were strongly preferred by T. ni. Caterpillars also preferred leaves with trichomes removed to leaves with trichomes intact. Differences among maternal lines indicate that phenotypic variation in defenses likely has a genetic basis. Furthermore, these results reveal that the feeding behaviors of T. ni map onto variation in plant defense in a predictable way. This work highlights the importance of variation in host-plant quality in driving interactions between plants and their herbivores. C1 [Alba, Christina; Hufbauer, Ruth A.] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Alba, Christina; Hufbauer, Ruth A.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Bowers, M. Deane] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Blumenthal, Dana] USDA ARS, Ft Collins, CO 80522 USA. RP Alba, C (reprint author), Acad Sci Czech Republic, Inst Bot, CS-25243 Pruhonice, Czech Republic. EM christina.alba@ibot.cas.cz RI Alba, Christina/H-1579-2014 OI Alba, Christina/0000-0001-7040-8480 FU United States Department of Agriculture, Colorado Agricultural Experiment Station [COL00680, COL00222A]; Harold David Harrington Graduate Fellowship; Colorado State University; Academy of Sciences of the Czech Republic [RVO 67985939] FX RH was supported by the United States Department of Agriculture, Colorado Agricultural Experiment Station (grant numbers COL00680 and COL00222A; URL aes.agsci.colostate.edu). CA received support from the Harold David Harrington Graduate Fellowship administered through the Biology Department at Colorado State University. Funding for page charges was provided by the Colorado State University Libraries Open Access Research and Scholarship Fund. CA was funded by long-term research development project no. RVO 67985939 from the Academy of Sciences of the Czech Republic URL http://cas.cz) during the writing of this paper. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the of the manuscript. NR 71 TC 4 Z9 4 U1 4 U2 43 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 15 PY 2014 VL 9 IS 8 AR e104889 DI 10.1371/journal.pone.0104889 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN8UD UT WOS:000340879300060 PM 25127229 ER PT J AU Golo, PS Gardner, DR Grilley, MM Takemoto, JY Krasnoff, SB Pires, MS Fernandes, EKK Bittencourt, VREP Roberts, DW AF Golo, Patricia S. Gardner, Dale R. Grilley, Michelle M. Takemoto, Jon Y. Krasnoff, Stuart B. Pires, Marcus S. Fernandes, Everton K. K. Bittencourt, Vania R. E. P. Roberts, Donald W. TI Production of Destruxins from Metarhizium spp. Fungi in Artificial Medium and in Endophytically Colonized Cowpea Plants SO PLOS ONE LA English DT Article ID ANISOPLIAE; INSECT; ROBERTSII; PATHOGENICITY; METABOLITES; INHIBITION; HEMOCYTES AB Destruxins (DTXs) are cyclic depsipeptides produced by many Metarhizium isolates that have long been assumed to contribute to virulence of these entomopathogenic fungi. We evaluated the virulence of 20 Metarhizium isolates against insect larvae and measured the concentration of DTXs A, B, and E produced by these same isolates in submerged (shaken) cultures. Eight of the isolates (ARSEF 324, 724, 760, 1448, 1882, 1883, 3479, and 3918) did not produce DTXs A, B, or E during the five days of submerged culture. DTXs were first detected in culture medium at 2-3 days in submerged culture. Galleria mellonella and Tenebrio molitor showed considerable variation in their susceptibility to the Metarhizium isolates. The concentration of DTXs produced in vitro did not correlate with percent or speed of insect kill. We established endophytic associations of M. robertsii and M. acridum isolates in Vigna unguiculata (cowpeas) and Cucumis sativus (cucumber) plants. DTXs were detected in cowpeas colonized by M. robertsii ARSEF 2575 12 days after fungal inoculation, but DTXs were not detected in cucumber. This is the first instance of DTXs detected in plants endophytically colonized by M. robertsii. This finding has implications for new approaches to fungus-based biological control of pest arthropods. C1 [Golo, Patricia S.; Pires, Marcus S.; Bittencourt, Vania R. E. P.] Univ Fed Rural Rio de Janeiro, Inst Vet, Dept Parasitol Anim, Seropedica, RJ, Brazil. [Golo, Patricia S.; Grilley, Michelle M.; Takemoto, Jon Y.; Roberts, Donald W.] Utah State Univ, Dept Biol, Logan, UT 84322 USA. [Gardner, Dale R.] ARS, USDA, Poisonous Plants Res Lab, Logan, UT USA. [Krasnoff, Stuart B.] USDA ARS, Biol Integrated Pest Management Res Unit, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Fernandes, Everton K. K.] Univ Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go, Brazil. RP Golo, PS (reprint author), Univ Fed Rural Rio de Janeiro, Inst Vet, Dept Parasitol Anim, Seropedica, RJ, Brazil. EM patriciagolo@gmail.com RI Bittencourt, Vania/D-3219-2013; Golo, Patricia/E-6469-2015; Fernandes, Everton/I-4890-2012; Takemoto, Jon/A-5309-2011 OI Golo, Patricia/0000-0003-1854-7488; Fernandes, Everton/0000-0001-7062-3295; Takemoto, Jon/0000-0001-9919-9168 FU United States Department of Agriculture (USDA)/Animal Plant Health and Inspection Service (APHIS) [13-8130-0114-CA] FX This research was supported in part by grants from the United States Department of Agriculture (USDA)/Animal Plant Health and Inspection Service (APHIS); Cooperative agreement (CA) #13-8130-0114-CA. The financial support was received by DWR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 4 Z9 4 U1 3 U2 57 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 15 PY 2014 VL 9 IS 8 AR e104946 DI 10.1371/journal.pone.0104946 PG 9 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN8UD UT WOS:000340879300062 PM 25127450 ER PT J AU Root, JJ Bentler, KT Shriner, SA Mooers, NL VanDalen, KK Sullivan, HJ Franklin, AB AF Root, J. Jeffrey Bentler, Kevin T. Shriner, Susan A. Mooers, Nicole L. VanDalen, Kaci K. Sullivan, Heather J. Franklin, Alan B. TI Ecological Routes of Avian Influenza Virus Transmission to a Common Mesopredator: An Experimental Evaluation of Alternatives SO PLOS ONE LA English DT Article ID LINKED-IMMUNOSORBENT-ASSAY; A VIRUSES; PATHOGENICITY; ANTIBODIES; INFECTION; POPULATIONS; PERSISTENCE; SUBTYPES; WATER; H7N1 AB Background: Wild raccoons have been shown to be naturally exposed to avian influenza viruses (AIV). However, the mechanisms associated with these natural exposures are not well-understood. Methodology/Principal Findings: We experimentally tested three alternative routes (water, eggs, and scavenged waterfowl carcasses) of AIV transmission that may explain how raccoons in the wild are exposed to AIV. Raccoons were exposed to 1) water and 2) eggs spiked with an AIV (H4N6), as well as 3) mallard carcasses experimentally inoculated with the same virus. Three of four raccoons exposed to the high dose water treatment yielded apparent nasal shedding of >10(2.0) PCR EID50 equivalent/mL. Little to no shedding was observed from the fecal route. The only animals yielding evidence of serologic activity during the study period were three animals associated with the high dose water treatment. Conclusions/Significance: Overall, our results indicate that virus-laden water could provide a natural exposure route of AIV for raccoons and possibly other mammals associated with aquatic environments. However, this association appears to be related to AIV concentration in the water, which would constitute an infective dose. In addition, strong evidence of infection was only detected in three of four animals exposed to a high dose (e.g., 10(5.0) EID50/mL) of AIV in water. As such, water-borne transmission to raccoons may require repeated exposures to water with high concentrations of virus. C1 [Root, J. Jeffrey; Bentler, Kevin T.; Shriner, Susan A.; Mooers, Nicole L.; VanDalen, Kaci K.; Sullivan, Heather J.; Franklin, Alan B.] USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80526 USA. RP Root, JJ (reprint author), USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80526 USA. EM Jeff.root@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182 FU United States Department of Agriculture FX This study was funded by the United States Department of Agriculture. The manuscript was reviewed for general policy statements committing the USDA to action, but otherwise the findings were independently developed by the authors. NR 34 TC 4 Z9 4 U1 0 U2 5 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 15 PY 2014 VL 9 IS 8 AR e102964 DI 10.1371/journal.pone.0102964 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN8UD UT WOS:000340879300006 PM 25127468 ER PT J AU Hassan, MA Robinson, SVJ Voepel, H Lewis, J Lisle, TE AF Hassan, Marwan A. Robinson, Samuel V. J. Voepel, Hal Lewis, Jack Lisle, Thomas E. TI Modeling temporal trends in bedload transport in gravel-bed streams using hierarchical mixed-effects models SO GEOMORPHOLOGY LA English DT Article DE Bedload; Sediment transport; Hierarchical mixed-effects modeling; Fluvial geomorphology, sediment supply; Gravel-bed streams ID FLUVIAL SEDIMENT TRANSPORT; MOUNTAIN STREAM; LOAD TRANSPORT; CHANNELS; RIVERS; FLOWS; RATES; ENTRAINMENT; MOBILITY; STORAGE AB In this paper, we used a bedload transport data set collected at North Fork Caspar Creek, California, to examine temporal variation in sediment transport rate over a 7-year period. Using a hierarchical mixed-effects model, we examined across and within-event variation to determine whether the bedload-shear stress relation trends over time. The relation between bedload transport and shear stress was modeled using log(Q(b)) = alpha + beta*log(T) + epsilon, where alpha and beta are constants and epsilon is an error term. Depending on the length of observation, alpha and beta can vary over several orders of magnitude, making modeling of transport based on flow challenging and highly inaccurate. We found a higher order yearly relation between bedload and shear stress, indicating systematic changes to the system over time. In the absence of significant additions to the system, alpha decreases roughly linearly over time, while beta does not show any trend. From the systematic decline in alpha, we infer changes to sediment availability in the stream overtime. Mixed-effects models have the potential to be a useful predictive tool in fluvial geomorphology, as they are more powerful at detecting trends in sediment transport rates than individual linear regressions. (C) 2014 Elsevier B.V. All rights reserved. C1 [Hassan, Marwan A.; Robinson, Samuel V. J.] Univ British Columbia, Dept Geog, Vancouver, BC V6T 1Z2, Canada. [Voepel, Hal] Desert Res Inst, Div Hydrol Sci, Reno, NV 89512 USA. [Lewis, Jack; Lisle, Thomas E.] US Forest Serv, Pacific NW Res Stn, USDA, Arcata, CA 95521 USA. RP Hassan, MA (reprint author), Univ British Columbia, Dept Geog, Vancouver, BC V6T 1Z2, Canada. EM marwan.hassan@geog.ubc.ca RI Voepel, Hal/K-7041-2014 OI Voepel, Hal/0000-0001-7375-1460 NR 56 TC 1 Z9 1 U1 5 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-555X EI 1872-695X J9 GEOMORPHOLOGY JI Geomorphology PD AUG 15 PY 2014 VL 219 BP 260 EP 269 DI 10.1016/j.geomorph.2014.05.019 PG 10 WC Geography, Physical; Geosciences, Multidisciplinary SC Physical Geography; Geology GA AM3TG UT WOS:000339775100021 ER PT J AU Lin, CW Chen, MH Yao, E Song, H Gacayan, R Hui, CC Chuang, PT AF Lin, Chuwen Chen, Miao-Hsueh Yao, Erica Song, Hai Gacayan, Rhodora Hui, Chi-chung Chuang, Pao-Tien TI Differential regulation of Gli proteins by Sufu in the lung affects PDGF signaling and myofibroblast development SO DEVELOPMENTAL BIOLOGY LA English DT Article DE Hedgehog; Sufu; Gli; Lung; Myofibroblast; PDGF ID SONIC HEDGEHOG; CHROMATIN IMMUNOPRECIPITATION; TRANSCRIPTION FACTOR; PATHWAY ACTIVATION; FLOOR PLATE; SUPPRESSOR; MOUSE; MECHANISMS; TRANSDUCTION; LIMB AB Mammalian Hedgehog (Hh) signaling relies on three Gli transcription factors to mediate Hh responses. This process is controlled in part by a major negative regulator, Sufu, through its effects on Gli protein level, distribution and activity. In this report, we showed that Sufu regulates Gli1 protein levels by antagonizing Numb/Itch. Otherwise, Numb/Itch would induce Gli1 protein degradation. This is in contrast to inhibition of Spop-mediated degradation of Gli2/3 by Sufu. Thus, controlling protein levels of all three Gli genes by Sufu is a conserved mechanism to modulate Hh responses albeit via distinct pathways. These findings in cell-based assays were further validated in vivo. In analyzing how Sufu controls Gli proteins in different tissues, we discovered that loss of Sufu in the lung exerts different effects on Hh target genes. Hh targets Ptch1/Hhip are upregulated in Sufu-deficient lungs, consistent with Hh pathway activation. Surprisingly, protein levels of Hh target Gli1 are reduced. We also found that myofibroblasts are absent from many prospective alveoli of Sufu-deficient lungs. Myofibroblast development is dependent on PDGF signaling. Interestingly, analysis of the Pdgfra promoter revealed a canonical Gli-binding site where Gli1 resides. These studies support a model in which loss of Sufu contributes to compromised Pdgfra activation and disrupts myofibroblast development in the lung. Our work illustrates the unappreciated complexity of Hh responses where distinct Hh targets could respond differently depending on the availability of Gli proteins that control their expression. (C) 2014 Elsevier Inc. All rights reserved. C1 [Lin, Chuwen; Chen, Miao-Hsueh; Yao, Erica; Song, Hai; Gacayan, Rhodora; Chuang, Pao-Tien] Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94158 USA. [Chen, Miao-Hsueh] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Hui, Chi-chung] Hosp Sick Children, Program Dev & Stem Cell Biol, Toronto, ON, Canada. [Hui, Chi-chung] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada. RP Chuang, PT (reprint author), Univ Calif San Francisco, Cardiovasc Res Inst, San Francisco, CA 94158 USA. EM pao-tien.chuang@ucsf.edu FU Canadian Cancer Society Research Institute [2011-700774]; National Institutes of Health [R01 HL091915] FX We thank David Ornitz for providing Dermo1Cre mice, Brian Black for the modified pGL-Basic vector, Vivian Chen and Chen-Che Huang for technical assistance, members of the Chuang laboratory for discussion and Ross Metzger for critical reading of the manuscript. This work was supported by grants from the Canadian Cancer Society Research Institute (Grant No. 2011-700774) to C.C. H. and the National Institutes of Health (R01 HL091915) to P.T. C. NR 57 TC 4 Z9 4 U1 1 U2 10 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0012-1606 EI 1095-564X J9 DEV BIOL JI Dev. Biol. PD AUG 15 PY 2014 VL 392 IS 2 BP 324 EP 333 DI 10.1016/j.ydbio.2014.05.014 PG 10 WC Developmental Biology SC Developmental Biology GA AM0KK UT WOS:000339534300016 PM 24886827 ER PT J AU Dathe, A Fleisher, DH Timlin, DJ Fisher, JK Reddy, VR AF Dathe, A. Fleisher, D. H. Timlin, D. J. Fisher, J. K. Reddy, V. R. TI Modeling potato root growth and water uptake under water stress conditions SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE Potato model; Convective-diffusive root model; Drought; Soil water transport; Transpiration ID ELEVATED CARBON-DIOXIDE; HYDRAULIC ARCHITECTURE; CORTICAL AERENCHYMA; INTEGRATED APPROACH; POROUS-MEDIA; GAS-EXCHANGE; PLANT-ROOTS; SOIL; SYSTEMS; MAIZE AB Potato (Solanum tuberosum L) is considered a drought sensitive crop. Accurate simulation of root growth is critical for estimating water uptake dynamics. However, data required to build and test advanced potato root simulation approaches is lacking. Previously unpublished data from our soil-plant-atmosphere-research (SPAR) chambers was used to evaluate a new two-dimensional diffusive root growth module linked to the existing potato model SPUDSIM. The root module consisted of diffusive parameters controlling the direction of root growth in horizontal and vertical directions, and an additional convective term for vertical growth within the soil. This modified SPUDSIM was tested against observed SPAR data which consisted of root distribution in the soil profile and organ dry weights (DW) at harvest, plus daily water uptake patterns for each of six different irrigation treatments. The difference between simulated and observed DW data was within two standard errors for most plant organs-root DW was over-predicted for well-watered plants. Spatial and temporal patterns of root distribution and water contents were reproduced well. However, the model tended to over-estimate water uptake from soil layers closer to the surface. Differences in simulated root growth patterns among irrigation treatments were the result of fluctuations in soil water status, bulk density, and root density which, in turn, affected the amount of carbon allocated to the roots in different soil layers and the value of the convective term. These results suggest the new module will provide more reliable predictions of potato water uptake for improved agricultural decision support tools. (C) 2014 Elsevier B.V. All rights reserved. C1 [Dathe, A.] Univ Maryland, Wye Res & Educ Ctr, Queenstown, MD 21658 USA. [Fleisher, D. H.; Timlin, D. J.; Fisher, J. K.; Reddy, V. R.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. RP Dathe, A (reprint author), Norwegian Univ Life Sci, Dept Environm Sci, POB 5003, N-1432 As, Norway. EM annette.dathe@nmbu.no NR 55 TC 3 Z9 3 U1 1 U2 69 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD AUG 15 PY 2014 VL 194 BP 37 EP 49 DI 10.1016/j.agrformet.2014.03.011 PG 13 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AL4UX UT WOS:000339131400005 ER PT J AU Fang, QX Ma, L Flerchinger, GN Qi, Z Ahuja, LR Xing, HT Lie, J Yu, Q AF Fang, Q. X. Ma, L. Flerchinger, G. N. Qi, Z. Ahuja, L. R. Xing, H. T. Lie, J. Yu, Q. TI Modeling evapotranspiration and energy balance in a wheat-maize cropping system using the revised RZ-SHAW model SO AGRICULTURAL AND FOREST METEOROLOGY LA English DT Article DE RZWQM; SHAW; Modeling; Agricultural systems; Energy balance ID NORTH CHINA PLAIN; WATER-USE EFFICIENCY; SOIL-WATER; CANOPY; SURFACE; RZWQM; TEMPERATURE; FLUXES; GROUNDWATER; SIMULATION AB Correctly simulating evapotranspiration (ET) and surface energy balance is essential to simulating crop growth under water and heat stress conditions in agricultural systems. The revised hybrid model (RZSHAW), combining the Root Zone Water Quality Model (RZWQM) and Simultaneous Heat and Water (SHAW) model, was evaluated for simulating ET and surface energy balance components against observed data from an eddy covariance system in a wheat-maize double cropping system in the North China Plain (NCP), after it was calibrated for soil water content and crop growth. The average daily ET was slightly under-simulated by 0.05 mm in the wheat seasons and over-simulated by 0.23 mm in the maize seasons, compared with the observed latent heat flux (LE) from 2003 to 2005. The root mean squared error (RMSE) and model efficiency (ME) of simulated daily ET were 0.59 mm and 0.86 for the three years. The goodness of simulation for Rn (net radiation), LE, H (sensible heat flux) and canopy temperature was better in the middle crop seasons than in the early crop seasons. The RMSE values for simulated Rn, H, LE, G (ground heat flux), and canopy temperature were 31.9, 37.2, 37.9, 21.8 W m-2, and 1.37 C, respectively, for middle wheat seasons and were 29.2, 27.1, 29.7, 19.7W m-2, and 1.22 C, respectively, for middle maize seasons. These simulation results were comparable with previous modeling studies, indicating that the revised hybrid model is reasonable for simulating B.V. C1 [Fang, Q. X.] Qingdao Agr Univ, Qingdao 266109, Peoples R China. [Ma, L.; Ahuja, L. R.] USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. [Flerchinger, G. N.] USDA ARS, Northwest Watershed Res Ctr, Boise, ID 83712 USA. [Qi, Z.] McGill Univ, Dept Bioresource Engn, Montreal, PQ H3A 2T5, Canada. [Xing, H. T.; Lie, J.; Yu, Q.] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. RP Ma, L (reprint author), USDA ARS, Agr Syst Res Unit, Ft Collins, CO 80526 USA. EM Liwang.Ma@ars.usda.gov RI Xing, Hongtao/I-4719-2013; Yu, Qiang/D-3702-2009 OI Xing, Hongtao/0000-0001-6733-162X; Yu, Qiang/0000-0001-6950-1821 NR 50 TC 9 Z9 11 U1 4 U2 52 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1923 EI 1873-2240 J9 AGR FOREST METEOROL JI Agric. For. Meteorol. PD AUG 15 PY 2014 VL 194 BP 218 EP 229 DI 10.1016/j.agrformet.2014.04.009 PG 12 WC Agronomy; Forestry; Meteorology & Atmospheric Sciences SC Agriculture; Forestry; Meteorology & Atmospheric Sciences GA AL4UX UT WOS:000339131400020 ER PT J AU Kroll, AJ Ren, Y Jones, JE Giovanini, J Perry, RW Thill, RE White, D Wigley, TB AF Kroll, Andrew J. Ren, You Jones, Jay E. Giovanini, Jack Perry, Roger W. Thill, Ronald E. White, Don, Jr. Wigley, T. Bently TI Avian community composition associated with interactions between local and landscape habitat attributes SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Avian communities; Hierarchical models; Intensive forestry; Landscape composition; Occupancy; Ouachita Mountains ID OREGON COAST RANGE; PACIFIC-NORTHWEST; MANAGED FORESTS; SPECIES RICHNESS; PLANTATION FORESTS; MIGRATORY BIRDS; BREEDING BIRDS; POINT COUNTS; FRAGMENTATION; BIODIVERSITY AB As human demand for ecosystem products increases, managers of landscapes used for commodity production require information about effects of management regimes on biological diversity. Landscape attributes, however, may moderate ecological responses to local-scale conservation and management actions. As a result, uniform application of local management prescriptions may yield variable biodiversity responses. We examined how interactions between local habitat structure and landscape forest cover were associated with avian community composition in the Ouachita Mountains, Arkansas, USA, 1995-1998. We used Bayesian hierarchical models to estimate occupancy for 63 breeding bird species, while accounting for variable detection with data collected from 1941 temporally replicated point count stations. Specifically, we estimated how interactions of four local habitat covariates (canopy cover of mature coniferous and hardwood trees, number of snags, and shrub cover) with percentage of mature hardwood forest at the landscape scale were associated with species occupancy and richness. Average predictive comparisons indicated that snag count and shrub cover had the strongest associations with species richness. Estimated associations for each of the four local forest cover variables was similar across all levels of landscape forest cover, suggesting weak or negligible interactions between these local measures and the landscape covariate. We found little support for our main prediction that local/landscape habitat interactions would be strongest at low levels of landscape forest cover (1-20%). Consequently, we suggest that forest managers consider prescriptions that result in a broad spatial distribution of heterogeneous habitat structural conditions (e.g., variation in understory cover and composition), irrespective of landscape context, to maintain a diverse avian breeding assemblage on landscapes in this region. (C) 2014 Elsevier B.V. All rights reserved. C1 [Kroll, Andrew J.; Ren, You; Jones, Jay E.; Giovanini, Jack] Weyerhaeuser NR, Federal Way, WA 98063 USA. [Perry, Roger W.] US Forest Serv, Southern Res Stn, USDA, Hot Springs, AR 71902 USA. [Thill, Ronald E.] US Forest Serv, Southern Res Stn, USDA, Nacogdoches, TX 75965 USA. [White, Don, Jr.] Univ Arkansas Monticello, Arkansas Forest Resource Ctr, Univ Arkansas, Agr Expt Stn, Monticello, AR 71656 USA. [Wigley, T. Bently] Natl Council Air & Stream Improvement Inc, Clemson, SC 29634 USA. RP Kroll, AJ (reprint author), Weyerhaeuser NR, POB 9777, Federal Way, WA 98063 USA. EM aj.kroll@weyerhaeuser.com FU U.S. Forest Service (Southern Research Station and Ouachita National Forest); Weyerhaeuser Company; National Council for Air and Stream Improvement; University of Arkansas Agricultural Experiment Station FX We thank M. Sams and H. Garner for coordinating field work and the many personnel that collected data for this study. We thank D. Miller, T. Melchiors, P. Tappe, and C. Watt for valuable input into different stages of the project. Funding was provided by the U.S. Forest Service (Southern Research Station and Ouachita National Forest), Weyerhaeuser Company, the National Council for Air and Stream Improvement, and the University of Arkansas Agricultural Experiment Station. NR 66 TC 5 Z9 5 U1 4 U2 71 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2014 VL 326 BP 46 EP 57 DI 10.1016/j.foreco.2014.04.011 PG 12 WC Forestry SC Forestry GA AL4UG UT WOS:000339129700006 ER PT J AU Rambo, T Schowalter, T North, M AF Rambo, Thomas Schowalter, Timothy North, Malcolm TI Canopy arthropod responses to thinning and burning treatments in old-growth mixed-conifer forest in the Sierra Nevada, California SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Arthropods; Fuels treatments; Sierra Nevada; Mixed-conifer forest; Forest thinning ID DOUGLAS-FIR; INVERTEBRATE COMMUNITIES; WESTERN OREGON; INSECTS; ASSEMBLAGES; ECOSYSTEMS; REGULATORS; OUTBREAKS; DENSITY; PATTERN AB We compared canopy arthropod responses to common fuels reduction treatments at Teakettle Experimental Forest in the south-central Sierra Nevada of California. We sampled arthropod communities among four dominant overstory conifer species and three dominant understory angiosperm species before and after overstory or understory thinning or no thinning treatments followed by burning or no burning treatments. Arthropods were sampled in overstory trees by climbing and bagging foliage-bearing branches and counting all arthropods by taxon in each sample. Understory plants were sampled similarly from the ground. Arthropod assemblages showed significant differences among tree species and seasons, but not among treatment combinations. Taxa showing significant differences in abundance among plant species likely reflected differences in foliage quality or other host-associated conditions among plant species. Some arthropods showed significant value as indicator species. Overall, our results indicated that the restoration treatments recommended for Sierra Nevada mixed-conifer forests have little effect on associated canopy arthropods. However, given the significant differences in arthropod assemblages among plant species, restoration treatments should ensure that the full range of plant species characterizing these forests is maintained in order to protect their associated arthropods. (C) 2014 Elsevier B.V. All rights reserved. C1 [Rambo, Thomas] Univ Calif Davis, John Muir Inst Environm, Forest Biol Res Ctr, Davis, CA 95616 USA. [Schowalter, Timothy] Louisiana State Univ, Ctr Agr, Dept Entomol, Baton Rouge, LA 70803 USA. [North, Malcolm] US Forest Serv, USDA, PSW Res Stn, Davis, CA 95618 USA. RP Rambo, T (reprint author), Univ Calif Davis, John Muir Inst Environm, Forest Biol Res Ctr, Davis, CA 95616 USA. EM trambo@ucdavis.edu; tschowalter@agcenter.lsu.edu; mpnorth@ucdavis.edu NR 37 TC 1 Z9 1 U1 3 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD AUG 15 PY 2014 VL 326 BP 91 EP 100 DI 10.1016/j.foreco.2014.04.014 PG 10 WC Forestry SC Forestry GA AL4UG UT WOS:000339129700009 ER PT J AU Ashby, RD Solaiman, DKY AF Ashby, Richard D. Solaiman, Daniel K. Y. TI Sophorolipid-Induced Dimpling and Increased Porosity in Solvent-Cast Short-Chain Polyhydroxyalkanoate Films: Impact on Thermomechanical Properties SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE biopolymers and renewable polymers; films; mechanical properties; properties and characterization; surfactants ID TISSUE ENGINEERING APPLICATIONS; PSEUDOMONAS-OLEOVORANS; CANCER CELLS; IN-VIVO; BIOSURFACTANTS; BLENDS; LENGTH; IMPLANTATION; COMPOSITES; SCAFFOLDS AB Sophorolipids (SL; microbial glycolipids) were used as additives in solvent-cast short-chain polyhydroxyalkanoate (sc-PHA) films to enhance surface roughness and porosity. Poly-3-hydroxybutyrate (PHB), poly-(6%)-3-hydroxybutyrate-co-(94%)-3-hydroxyvalerate (PHB/V), and poly-(90%)-3-hydroxybutyrate-co-(10%)-3-hydroxyhexanoate (PHB/HHx) films were evaluated with up to 43 wt % of SL. Sophorolipid addition caused surface dimples with maximum diameters of 131.8 mu m (PHB), 25.2 mu m (PHB/V), and 102.8 mu m (PHB/HHx). A rise in the size and number of pores in the polymer matrix also occurred in PHB and PHB/V films. Surface roughness and film porosity were visualized by scanning electron microscopy and quantitated using confocal microscopy by correlating the surface area (A) to the scanned area (A) of the films. The phenotypic alterations of the films caused a gradual decline in tensile strength and modulus and increased the elongation to break. Reductions in the enthalpies of fusion (H-f) in both the PHB (41% reduction) and PHB/HHx (36% reduction) films implied diminished crystallinity as SL concentrations increased. Over the same SL concentrations the Tan maxima shifted from 4 to 30 degrees C and from 2 to 20 degrees C in these respective films. These results provide a novel means by which sc-PHA properties can be controlled for new/improved applications. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40609. C1 [Ashby, Richard D.; Solaiman, Daniel K. Y.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Ashby, RD (reprint author), ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. EM rick.ashby@ars.usda.gov NR 46 TC 3 Z9 3 U1 0 U2 19 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD AUG 15 PY 2014 VL 131 IS 16 AR 40609 DI 10.1002/app.40609 PG 11 WC Polymer Science SC Polymer Science GA AH9HV UT WOS:000336453600013 ER PT J AU Sun, QN Hse, CY Shupe, TF AF Sun, Qi-Ning Hse, Chung-Yun Shupe, Todd F. TI Effect of Different Catalysts on Urea-Formaldehyde Resin Synthesis SO JOURNAL OF APPLIED POLYMER SCIENCE LA English DT Article DE addition polymerization; adhesives; catalysts; structure-property relations; synthesis and processing ID C-13 NMR-SPECTROSCOPY; SYNTHESIS PARAMETERS; WOOD ADHESIVES; MELAMINE; POLYMER AB Four catalysts (H2SO4, HCl, H3PO4, and NaOH/NH4OH) were studied in the preparation of melamine modified urea-formaldehyde (UFM) resins. C-13-nuclear magnetic resonance spectroscopic analysis of the UFM resins at different synthesis stages revealed the polymer structure and detailed reaction mechanism. Three acidic catalysts (H2SO4, HCl, and H3PO4) enhanced the resin polymerization through the formation of various contents of methylene, ether linkages, and urons. H3PO4 yielded the most terminal ether linkages at the first stage and enhanced polycondensation by depleting all free urea and glycols to form the most linear methylene linkages NHCH2NH in the end. However, at the initial synthesis stage, NaOH/NH4OH catalyzed the formation of UFM prepolymer to a limited extent with a large amount of free urea left, and therefore produced the final polymer with relatively more substituted methylolureas and linear ether linkages. (c) 2014 Wiley Periodicals, Inc. C1 [Sun, Qi-Ning; Shupe, Todd F.] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA. [Hse, Chung-Yun] US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. RP Hse, CY (reprint author), US Forest Serv, USDA, Southern Res Stn, Pineville, LA 71360 USA. EM chse@fs.fed.us RI Sun, Qining/B-7592-2016 OI Sun, Qining/0000-0002-9678-7834 NR 18 TC 4 Z9 4 U1 5 U2 47 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8995 EI 1097-4628 J9 J APPL POLYM SCI JI J. Appl. Polym. Sci. PD AUG 15 PY 2014 VL 131 IS 16 AR 40644 DI 10.1002/app.40644 PG 7 WC Polymer Science SC Polymer Science GA AH9HV UT WOS:000336453600048 ER PT J AU Cabanillas, B Maleki, SJ Rodriguez, J Cheng, H Teuber, SS Wallowitz, ML Muzquiz, M Pedrosa, MM Linacero, R Burbano, C Novak, N Cuadrado, C Crespo, JF AF Cabanillas, Beatriz Maleki, Soheila J. Rodriguez, Julia Cheng, Hsiaopo Teuber, Suzanne S. Wallowitz, Mikhael L. Muzquiz, Mercedes Pedrosa, Mercedes M. Linacero, Rosario Burbano, Carmen Novak, Natalija Cuadrado, Carmen Crespo, Jesus F. TI Allergenic properties and differential response of walnut subjected to processing treatments SO FOOD CHEMISTRY LA English DT Article DE High pressure; Processing; Thermal treatments; Tree nut allergy; Walnut allergy ID CONTROLLED PRESSURE-DROP; IGE-BINDING EPITOPES; TREE NUT ALLERGENS; CROSS-REACTIVITY; PEANUT; PROTEINS; HAZELNUT; RELEASE; GLOBULIN; CHILDREN AB The aim of this study was to investigate changes in walnut allergenicity after processing treatments by in vitro techniques and physiologically relevant assays. The allergenicity of walnuts subjected to high hydrostatic pressure and thermal/pressure treatments was evaluated by IgE-immunoblot and antibodies against walnut major allergen jug r 4. The ability of processed walnut to cross-link IgE on effector cells was evaluated using a rat basophil leukaemia cell line and by skin prick testing. Susceptibility to gastric and duodenal digestion was also evaluated. The results showed that walnuts subjected to pressure treatment at 256 kPa, 138 degrees C, were able to diminish the IgE cross-linking capacity on effector cells more efficiently than high pressure treated walnuts. IgE immunoblot confirmed these results. Moreover, higher susceptibility to digestion of pressure treated walnut proteins was observed. The use of processed walnuts with decreased IgE binding capacity could be a potential strategy for walnut tolerance induction. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Cabanillas, Beatriz; Novak, Natalija] Univ Bonn, Med Ctr, Dept Dermatol & Allergy, D-53127 Bonn, Germany. [Cabanillas, Beatriz; Rodriguez, Julia; Crespo, Jesus F.] Hosp Univ 12 Octubre, Inst Invest Hosp 12 Octubre I 12, Serv Alergia, Madrid 28041, Spain. [Maleki, Soheila J.; Cheng, Hsiaopo] ARS, USDA, So Reg Res Ctr, New Orleans, LA USA. [Teuber, Suzanne S.; Wallowitz, Mikhael L.] Univ Calif Davis, Sch Med, Div Rheumatol Allergy & Clin Immunol, Genome & Biomed Sci Facil, Davis, CA 95616 USA. [Muzquiz, Mercedes; Pedrosa, Mercedes M.; Burbano, Carmen; Cuadrado, Carmen] SGIT INIA, Dept Tecnol Alimentos, Madrid 28040, Spain. [Linacero, Rosario] Univ Complutense Madrid, Fac Biol, Dept Genet, E-28040 Madrid, Spain. RP Cabanillas, B (reprint author), Univ Bonn, Med Ctr, Dept Dermatol & Allergy, Sigmund Freud Str 25, D-53127 Bonn, Germany. EM Beatriz.Cabanillas@ukb.uni-bonn.de RI Pedrosa, Mercedes/O-2353-2014; Rodriguez Herrera, Juan Jose/I-3210-2015; CUADRADO, CARMEN/C-5504-2013; OI Pedrosa, Mercedes/0000-0002-8313-9790; CUADRADO, CARMEN/0000-0003-2609-1900; Cabanillas, Beatriz/0000-0002-5351-8140; Linacero, Rosario/0000-0002-9773-8444 FU Ministerio de Ciencia e Innovacion (Spain) [AGL2008-03453-C02]; Instituto Carlos III, Ministerio de Ciencia e Innovacion (Spain) [FI07/00286]; BON-FOR Grant of the University of Bonn (Germany) FX This study was supported by Grant AGL2008-03453-C02, Ministerio de Ciencia e Innovacion (Spain), Grant FI07/00286 Instituto Carlos III, Ministerio de Ciencia e Innovacion (Spain), and a BON-FOR Grant of the University of Bonn (Germany). NR 37 TC 14 Z9 17 U1 10 U2 71 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD AUG 15 PY 2014 VL 157 BP 141 EP 147 DI 10.1016/j.foodchem.2014.02.025 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AF8NT UT WOS:000334973600020 PM 24679763 ER PT J AU Greene, SL Kisha, TJ Yu, LX Parra-Quijano, M AF Greene, Stephanie L. Kisha, Theodore J. Yu, Long-Xi Parra-Quijano, Mauricio TI Conserving Plants in Gene Banks and Nature: Investigating Complementarity with Trifolium thompsonii Morton SO PLOS ONE LA English DT Article ID MULTILOCUS GENOTYPE DATA; ESTIMATING POPULATION-STRUCTURE; EX-SITU CONSERVATION; IN-SITU; MOLECULAR MARKERS; DOMINANT MARKERS; AFLP DATA; DIVERSITY; L.; WILD AB A standard conservation strategy for plant genetic resources integrates in situ (on-farm or wild) and ex situ (gene or field bank) approaches. Gene bank managers collect ex situ accessions that represent a comprehensive snap shot of the genetic diversity of in situ populations at a given time and place. Although simple in theory, achieving complementary in situ and ex situ holdings is challenging. Using Trifolium thompsonii as a model insect-pollinated herbaceous perennial species, we used AFLP markers to compare genetic diversity and structure of ex situ accessions collected at two time periods (1995, 2004) from four locations, with their corresponding in situ populations sampled in 2009. Our goal was to assess the complementarity of the two approaches. We examined how gene flow, selection and genetic drift contributed to population change. Across locations, we found no difference in diversity between ex situ and in situ samples. One population showed a decline in genetic diversity over the 15 years studied. Population genetic differentiation among the four locations was significant, but weak. Association tests suggested infrequent, long distance gene flow. Selection and drift occurred, but differences due to spatial effects were three times as strong as differences attributed to temporal effects, and suggested recollection efforts could occur at intervals greater than fifteen years. An effective collecting strategy for insect pollinated herbaceous perennial species was to sample >150 plants, equalize maternal contribution, and sample along random transects with sufficient space between plants to minimize intrafamilial sampling. Quantifying genetic change between ex situ and in situ accessions allows genetic resource managers to validate ex situ collecting and maintenance protocols, develop appropriate recollection intervals, and provide an early detection mechanism for identifying problematic conditions that can be addressed to prevent further decline in vulnerable in situ populations. C1 [Greene, Stephanie L.] USDA ARS, Natl Ctr Genet Resource Preservat, Ft Collins, CO 80522 USA. [Kisha, Theodore J.; Yu, Long-Xi] USDA ARS, Western Reg Plant Intro Stn, Pullman, WA USA. [Parra-Quijano, Mauricio] Univ Politecn Madrid, Dept Biol Vegetal, Madrid, Spain. RP Greene, SL (reprint author), USDA ARS, Natl Ctr Genet Resource Preservat, Ft Collins, CO 80522 USA. EM stephanie.greene@ars.usda.gov FU USDA Agricultural Research Service National Program [301, 215]; International Treaty on Plant Genetic Resources for Food and Agriculture - FAO FX Sources of funding included USDA Agricultural Research Service National Program 301 and 215 and the International Treaty on Plant Genetic Resources for Food and Agriculture - FAO. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 5 Z9 5 U1 2 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 14 PY 2014 VL 9 IS 8 AR e105145 DI 10.1371/journal.pone.0105145 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO0QT UT WOS:000341017000100 PM 25121602 ER PT J AU Zunino, SJ Peerson, JM Freytag, TL Breksa, AP Bonnel, EL Woodhouse, LR Storms, DH AF Zunino, Susan J. Peerson, Jan M. Freytag, Tammy L. Breksa, Andrew P., III Bonnel, Ellen L. Woodhouse, Leslie R. Storms, David H. TI Dietary grape powder increases IL-1 beta and IL-6 production by lipopolysaccharide-activated monocytes and reduces plasma concentrations of large LDL and large LDL-cholesterol particles in obese humans SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Grapes; Obesity; Lipids; Inflammation ID PERFORMANCE LIQUID-CHROMATOGRAPHY; NECROSIS-FACTOR-ALPHA; INSULIN-RESISTANCE; ANTIOXIDANT CAPACITY; METABOLIC SYNDROME; ADIPOSE-TISSUE; RISK-FACTORS; INFLAMMATION; MARKERS; IMPACT AB Obese individuals are at an increased risk of developing CVD, hypertension, type 2 diabetes, and bacterial and viral infections when compared with the normal-weight population. In a 9-week randomised, double-blind, cross-over study, twenty-four obese subjects aged between 20 and 60 years and with a BMI between 30 and 45 kg/m(2) were fed grape or placebo powder for 3-week intervals to determine the effects of dietary grapes on blood lipid profiles, plasma inflammatory marker concentrations and immune cell function. Blood samples were collected on days 1 and 8 for obtaining baseline information and at weeks 3, 4, 8 and 9. Comprehensive chemistry panels, lipid profile analyses by NMR, measurement of plasma inflammatory marker concentrations, and analyses of cytokine production by activated T lymphocytes and monocytes were performed for each blood draw. Dietary grape powder reduced the plasma concentrations of large LDL-cholesterol and large LDL particles compared with the placebo powder (P<0.05). The concentrations of interferon-gamma, TNF-alpha, IL-4 and IL-10 were measured in supernatants from peripheral blood mononuclear cells (PBMC) activated with anti-CD3/CD28 antibodies and those of TNF-alpha, IL-1 beta, IL-6 and IL-8 were measured in supernatants from PBMC activated with lipopolysaccharide (LPS). No difference in the production of T-cell cytokines was observed between the two intervention groups. The production of IL-1 beta and IL-6 was increased in supernatants from LPS-activated PBMC in the grape powder group compared with the placebo powder group (P<0.05). These data suggest that dietary grapes may decrease atherogenic lipid fractions in obese individuals and increase the sensitivity of monocytes in a population at a greater risk of developing infections. C1 [Zunino, Susan J.; Freytag, Tammy L.; Woodhouse, Leslie R.; Storms, David H.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Peerson, Jan M.; Bonnel, Ellen L.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Breksa, Andrew P., III] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. RP Zunino, SJ (reprint author), ARS, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM susan.zunino@ars.usda.gov FU United States Department of Agriculture (USDA) Current Research Information System project [5306-51530-018-00D]; California Table Grape Commission FX The present study was supported by the United States Department of Agriculture (USDA) Current Research Information System project no. 5306-51530-018-00D and a grant from the California Table Grape Commission. The USDA is an equal-opportunity provider and employer. The California Table Grape had no role in the design and analysis of the study or in the writing of this article. NR 45 TC 5 Z9 5 U1 1 U2 12 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD AUG 14 PY 2014 VL 112 IS 3 BP 369 EP 380 DI 10.1017/S0007114514000890 PG 12 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AL3UD UT WOS:000339054100009 PM 24832727 ER PT J AU Zhang, BC Luo, YG Pearlstein, AJ Aplin, J Liu, Y Bauchan, GR Payne, GF Wang, Q Nou, XW Millner, PD AF Zhang, Boce Luo, Yaguang Pearlstein, Arne J. Aplin, Jesse Liu, Yi Bauchan, Gary R. Payne, Gregory F. Wang, Qin Nou, Xiangwu Millner, Patricia D. TI Fabrication of Biomimetically Patterned Surfaces and Their Application to Probing Plant-Bacteria Interactions SO ACS APPLIED MATERIALS & INTERFACES LA English DT Article DE plant-bacteria interaction; biomimetically-patterned surfaces; replica molding; PDMS; agarose; surface topography ID ESCHERICHIA-COLI O157H7; FRESH-PRODUCE; LEAFY GREENS; INTERNALIZATION; BIOFILMS; ADHESION; WETTABILITY; RETENTION; ROUGHNESS; SYSTEMS AB We have developed a two-step replica molding method for rapid fabrication of biomimetically patterned plant surfaces (BPS) using polydimethylsiloxane (PDMS-BPS) and agarose (AGAR-BPS). Beyond providing multiple identical specimens that faithfully reproduce leaf surface microstructure, this approach also offers unique chemical, physical, and biological features. PDMS-BPS provide good structural durability for SEM examination, have surface wettability comparable to plant surfaces for coating development, and allow for real-time monitoring of biosynthesis through incorporation into microfluidic devices. AGAR-BPS are compatible with bacterial growth, recovery, and quantification, and enable investigation of the effects of surface topography on spatially varying survival and inactivation of Escherichia coli cells during biocide treatment. Further development and application of these biomimetically patterned surfaces to study (and possibly modify) other aspects of plant bacteria interactions can provide insight into controlling pathogen contamination in a wide range of applications. C1 [Zhang, Boce; Luo, Yaguang; Aplin, Jesse; Nou, Xiangwu; Millner, Patricia D.] ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. [Zhang, Boce; Aplin, Jesse; Wang, Qin] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Luo, Yaguang] ARS, Food Qual Lab, USDA, Beltsville, MD 20705 USA. [Pearlstein, Arne J.] Univ Illinois, Dept Mech Sci & Engn, Urbana, IL 61801 USA. [Liu, Yi; Payne, Gregory F.] Univ Maryland, Inst Biosci & Biotechnol Res, College Pk, MD 20742 USA. [Bauchan, Gary R.] ARS, Electron & Confocal Microscopy Unit, USDA, Beltsville, MD 20705 USA. [Payne, Gregory F.] Univ Maryland, Fischell Dept Bioengn, College Pk, MD 20742 USA. RP Luo, YG (reprint author), ARS, Environm Microbial & Food Safety Lab, USDA, Beltsville, MD 20705 USA. EM yaguang.luo@ars.usda.gov OI Pearlstein, Arne/0000-0002-7044-5537 FU USDA-NIFA Specialty Crop Research Initiative Grant [2010-01165]; U.S. Defense Threat Reduction Agency [HDTRA1-13-0037, BO085PO008]; FabLab at the Maryland Nano Center FX This work was supported by USDA-NIFA Specialty Crop Research Initiative Grant Award 2010-01165. We also acknowledge grant HDTRA1-13-0037 and BO085PO008 from the U.S. Defense Threat Reduction Agency. We are grateful for the support of the FabLab at the Maryland Nano Center. We thank Dr. William Bentley and Ms. Jessica Terrell of the Fischell Department of Bioengineering at the University of Maryland, College Park, for providing BFP-E. coli strains and for generous technical support. Access to the FACS equipment at the Flow Cytometry Core Facility (Maryland Pathogen Research Institute, University of Maryland) is gratefully acknowledged. NR 37 TC 7 Z9 8 U1 5 U2 39 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 1944-8244 J9 ACS APPL MATER INTER JI ACS Appl. Mater. Interfaces PD AUG 13 PY 2014 VL 6 IS 15 BP 12467 EP 12478 DI 10.1021/am502384q PG 12 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary SC Science & Technology - Other Topics; Materials Science GA AN2WI UT WOS:000340446300078 PM 25007271 ER PT J AU Kretchun, AM Scheller, RM Lucash, MS Clark, KL Hom, J Van Tuyl, S AF Kretchun, Alec M. Scheller, Robert M. Lucash, Melissa S. Clark, Kenneth L. Hom, John Van Tuyl, Steve TI Predicted Effects of Gypsy Moth Defoliation and Climate Change on Forest Carbon Dynamics in the New Jersey Pine Barrens SO PLOS ONE LA English DT Article ID LANDSCAPE MODELS; NORTH-AMERICA; UNITED-STATES; OUTBREAKS; SIMULATION; SCENARIOS; INSECTS; LANDIS; BEETLE; SINK AB Disturbance regimes within temperate forests can significantly impact carbon cycling. Additionally, projected climate change in combination with multiple, interacting disturbance effects may disrupt the capacity of forests to act as carbon sinks at large spatial and temporal scales. We used a spatially explicit forest succession and disturbance model, LANDIS-II, to model the effects of climate change, gypsy moth (Lymantria dispar L.) defoliation, and wildfire on the C dynamics of the forests of the New Jersey Pine Barrens over the next century. Climate scenarios were simulated using current climate conditions (baseline), as well as a high emissions scenario (HadCM3 A2 emissions scenario). Our results suggest that long-term changes in C cycling will be driven more by climate change than by fire or gypsy moths over the next century. We also found that simulated disturbances will affect species composition more than tree growth or C sequestration rates at the landscape level. Projected changes in tree species biomass indicate a potential increase in oaks with climate change and gypsy moth defoliation over the course of the 100-year simulation, exacerbating current successional trends towards increased oak abundance. Our research suggests that defoliation under climate change may play a critical role in increasing the variability of tree growth rates and in determining landscape species composition over the next 100 years. C1 [Kretchun, Alec M.; Scheller, Robert M.; Lucash, Melissa S.] Portland State Univ, Dept Environm Sci & Management, Portland, OR 97207 USA. [Clark, Kenneth L.] USDA, Forest Serv, Silas Little Expt Forest, New Lisbon, NJ USA. [Hom, John; Van Tuyl, Steve] USDA ARS, Newtown Sq, PA USA. RP Kretchun, AM (reprint author), Portland State Univ, Dept Environm Sci & Management, Portland, OR 97207 USA. EM AlecKretchun@Gmail.com RI Scheller, Robert/B-3135-2009 FU National Fire Plan, Forest Health Monitoring [NE-F-08-01]; Eastern Landfire Prototype FX This work was supported by the National Fire Plan, Forest Health Monitoring (NE-F-08-01), and the Eastern Landfire Prototype. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 44 TC 1 Z9 1 U1 5 U2 54 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 13 PY 2014 VL 9 IS 8 AR e102531 DI 10.1371/journal.pone.0102531 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN9BM UT WOS:000340900600008 PM 25119162 ER PT J AU Wang, J Sainju, UM AF Wang, Jun Sainju, Upendra M. TI Soil Carbon and Nitrogen Fractions and Crop Yields Affected by Residue Placement and Crop Types SO PLOS ONE LA English DT Article ID NORTHERN GREAT-PLAINS; LONG-TERM TILLAGE; MICROBIAL BIOMASS; ORGANIC-MATTER; MINERALIZABLE CARBON; ROTATION; SEQUENCE; SYSTEM; SEQUESTRATION; DYNAMICS AB Soil labile C and N fractions can change rapidly in response to management practices compared to non-labile fractions. High variability in soil properties in the field, however, results in nonresponse to management practices on these parameters. We evaluated the effects of residue placement (surface application [or simulated no-tillage] and incorporation into the soil [or simulated conventional tillage]) and crop types (spring wheat [Triticum aestivum L.], pea [Pisum sativum L.], and fallow) on crop yields and soil C and N fractions at the 0-20 cm depth within a crop growing season in the greenhouse and the field. Soil C and N fractions were soil organic C (SOC), total N (STN), particulate organic C and N (POC and PON), microbial biomass C and N (MBC and MBN), potential C and N mineralization (PCM and PNM), NH4-N, and NO3-N concentrations. Yields of both wheat and pea varied with residue placement in the greenhouse as well as in the field. In the greenhouse, SOC, PCM, STN, MBN, and NH4-N concentrations were greater in surface placement than incorporation of residue and greater under wheat than pea or fallow. In the field, MBN and NH4-N concentrations were greater in no-tillage than conventional tillage, but the trend reversed for NO3-N. The PNM was greater under pea or fallow than wheat in the greenhouse and the field. Average SOC, POC, MBC, PON, PNM, MBN, and NO3-N concentrations across treatments were higher, but STN, PCM and NH4-N concentrations were lower in the greenhouse than the field. The coefficient of variation for soil parameters ranged from 2.6 to 15.9% in the greenhouse and 8.0 to 36.7% in the field. Although crop yields varied, most soil C and N fractions were greater in surface placement than incorporation of residue and greater under wheat than pea or fallow in the greenhouse than the field within a crop growing season. Short-term management effect on soil C and N fractions were readily obtained with reduced variability under controlled soil and environmental conditions in the greenhouse compared to the field. Changes occurred more in soil labile than non-labile C and N fractions in the greenhouse than the field. C1 [Wang, Jun] Northwest Univ, Coll Urban & Environm Sci, Xian, Shaanxi Provinc, Peoples R China. [Sainju, Upendra M.] USDA ARS, Northeren Plains Agr Res Lab, Sidney, MN USA. RP Sainju, UM (reprint author), USDA ARS, Northeren Plains Agr Res Lab, Sidney, MN USA. EM upendra.sainju@ars.usda.gov FU U.S. Department of Agriculture-Agricultural Research Service, Sidney, MT, USA; National Natural Science Foundation of China [31270484] FX Funding came from the U.S. Department of Agriculture-Agricultural Research Service, Sidney, MT, USA and the National Natural Science Foundation of China (No. 31270484). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 36 TC 0 Z9 0 U1 0 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 13 PY 2014 VL 9 IS 8 AR e105039 DI 10.1371/journal.pone.0105039 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AN9BM UT WOS:000340900600117 PM 25119381 ER PT J AU Harnly, JM Harrington, PB Botros, LL Jablonski, J Chang, C Bergana, MM Wehling, P Downey, G Potts, AR Moore, JC AF Harnly, James M. Harrington, Peter B. Botros, Lucy L. Jablonski, Joseph Chang, Claire Bergana, Marti Mamula Wehling, Paul Downey, Gerard Potts, Alan R. Moore, Jeffrey C. TI Characterization of Near-Infrared Spectral Variance in the Authentication of Skim and Nonfat Dry Milk Powder Collection Using ANOVA-PCA, Pooled-ANOVA, and Partial Least-Squares Regression SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE pooled-ANOVA; milk powder; near-infrared spectroscopy; partial least-squares regression; ANOVA-PCA ID PRINCIPAL COMPONENT ANALYSIS; TOOL AB Forty-one samples of skim milk powder (SMP) and nonfat dry milk (NFDM) from 8 suppliers, 13 production sites, and 3 processing temperatures were analyzed by NIR diffuse reflectance spectrometry over a period of 3 days. NIR reflectance spectra (1700-2500 nm) were converted to pseudoabsorbance and examined using (a) analysis of variance-principal component analysis (ANOVA-PCA), (b) pooled-ANOVA based on data submatrices, and (c) partial least-squares regression (PLSR) coupled with pooled-ANOVA. ANOVA-PCA score plots showed clear separation of the samples with respect to milk class (SMP or NFDM), day of analysis, production site, processing temperature, and individual samples. Pooled-ANOVA provided statistical levels of significance for the separation of the averages, some of which were many orders of magnitude below 10(-3). PLSR showed that the correlation with Certificate of Analysis (COA) concentrations varied from a weak coefficient of determination (R-2) of 0.32 for moisture to moderate R2 values of 0.61 for fat and 0.78 for protein for this multinational study. In this study, pooled-ANOVA was applied for the first time to PLS modeling and demonstrated that even though the calibration models may not be precise, the contribution of the protein peaks in the NIR spectra accounted for the largest proportion of the variation despite the inherent imprecision of the COA values. C1 [Harnly, James M.] USDA, ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab,BARC East, Beltsville, MD 20817 USA. [Harrington, Peter B.] Ohio Univ, Dept Chem & Biochem, Ctr Intelligent Chem Instrumentat, Clippinger Labs, Athens, OH 45701 USA. [Botros, Lucy L.; Potts, Alan R.; Moore, Jeffrey C.] US Pharmacopeial Convent Inc, Rockville, MD 20852 USA. [Jablonski, Joseph; Chang, Claire] US FDA, Ctr Food Safety & Appl Nutr, Div Food Proc Sci & Technol, Bedford Pk, SA 60501, Australia. [Bergana, Marti Mamula] Abbott Nutr, Div Abbott Labs, Columbus, OH 43219 USA. [Wehling, Paul] Gen Mills Inc, Minneapolis, MN 55414 USA. [Downey, Gerard] Teagasc Food Res Ctr Ashtown, Dublin 15, Ireland. RP Harnly, JM (reprint author), USDA, ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab,BARC East, Bldg 161, Beltsville, MD 20817 USA. EM james.harnly@ars.usda.gov; peter.harrington@ohio.edu RI Downey, Gerard/G-5840-2011; OI Downey, Gerard/0000-0002-6633-1270; Harrington, Peter/0000-0003-0268-8630 FU Agricultural Research Service of the U.S. Department of Agriculture; Office of Dietary Supplements of the National Institutes of Health FX Walter Hauck and Markus Lipp from USP are thanked for their valuable comments. One of the authors (JMH) acknowledges support or the Agricultural Research Service of the U.S. Department of Agriculture and the Office of Dietary Supplements of the National Institutes of Health. NR 6 TC 3 Z9 3 U1 4 U2 30 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 13 PY 2014 VL 62 IS 32 BP 8060 EP 8067 DI 10.1021/jf5013727 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AN2UE UT WOS:000340440500010 PM 25010570 ER PT J AU Bevins, SN Pedersen, K Lutman, MW Baroch, JA Schmit, BS Kohler, D Gidlewski, T Nolte, DL Swafford, SR DeLiberto, TJ AF Bevins, Sarah N. Pedersen, Kerri Lutman, Mark W. Baroch, John A. Schmit, Brandon S. Kohler, Dennis Gidlewski, Thomas Nolte, Dale L. Swafford, Seth R. DeLiberto, Thomas J. TI Large-Scale Avian Influenza Surveillance in Wild Birds throughout the United States SO PLOS ONE LA English DT Article ID H5N1 VIRUS; A VIRUSES; MIGRATORY BIRDS; SOUTH-AMERICA; NORTH; WATERFOWL; PATTERNS; SUBTYPES; ECOLOGY; CANADA AB Avian influenza is a viral disease that primarily infects wild and domestic birds, but it also can be transmitted to a variety of mammals. In 2006, the United States of America Departments of Agriculture and Interior designed a large-scale, interagency surveillance effort that sought to determine if highly pathogenic avian influenza viruses were present in wild bird populations within the United States of America. This program, combined with the Canadian and Mexican surveillance programs, represented the largest, coordinated wildlife disease surveillance program ever implemented. Here we analyze data from 197,885 samples that were collected from over 200 wild bird species. While the initial motivation for surveillance focused on highly pathogenic avian influenza, the scale of the data provided unprecedented information on the ecology of avian influenza viruses in the United States, avian influenza virus host associations, and avian influenza prevalence in wild birds over time. Ultimately, significant advances in our knowledge of avian influenza will depend on both large-scale surveillance efforts and on focused research studies. C1 [Bevins, Sarah N.; Pedersen, Kerri; Lutman, Mark W.; Baroch, John A.; Schmit, Brandon S.; Kohler, Dennis; Gidlewski, Thomas; Nolte, Dale L.; Swafford, Seth R.; DeLiberto, Thomas J.] APHIS, Wildlife Serv, USDA, Natl Wildlife Dis Program, Ft Collins, CO 80526 USA. [Bevins, Sarah N.; Baroch, John A.; Schmit, Brandon S.; Kohler, Dennis; Gidlewski, Thomas; DeLiberto, Thomas J.] Natl Wildlife Res Ctr, APHIS, Wildlife Serv, USDA, Ft Collins, CO USA. [Pedersen, Kerri; Lutman, Mark W.; Nolte, Dale L.] APHIS, Wildlife Serv, USDA, Natl Feral Swine Damage Management Program, Ft Collins, CO USA. [Swafford, Seth R.] United States Fish & Wildlife Serv, USDOI, Yazoo City, MS USA. RP Bevins, SN (reprint author), APHIS, Wildlife Serv, USDA, Natl Wildlife Dis Program, Ft Collins, CO 80526 USA. EM Sarah.N.Bevins@aphis.usda.gov FU United States Department of Agriculture FX This study was funded by the United States Department of Agriculture. The manuscript was reviewed for general policy statements committing the USDA to action, but otherwise the findings were independently developed by the authors. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 8 Z9 8 U1 0 U2 26 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 12 PY 2014 VL 9 IS 8 AR e104360 DI 10.1371/journal.pone.0104360 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO3LJ UT WOS:000341230400043 PM 25116079 ER PT J AU Chao, WS Dogramaci, M Anderson, JV Foley, ME Horvath, DP AF Chao, Wun S. Dogramaci, Muenevver Anderson, James V. Foley, Michael E. Horvath, David P. TI The resemblance and disparity of gene expression in dormant and non-dormant seeds and crown buds of leafy spurge (Euphorbia esula) SO BMC PLANT BIOLOGY LA English DT Article DE Leafy spurge; Bud dormancy; Seed dormancy; Gene expression; Hormones; Transcription factors ID TRANSCRIPT PROFILES; AUXIN TRANSPORT; WEED BIOLOGY; GROWTH; ARABIDOPSIS; GERMINATION; CLASSIFICATION; TEMPERATURES; ENDODORMANCY; INHIBITION AB Background: Leafy spurge (Euphorbia esula L.) is a herbaceous perennial weed and dormancy in both buds and seeds is an important survival mechanism. Bud dormancy in leafy spurge exhibits three well-defined phases of para-, endo- and ecodormancy; however, seed dormancy for leafy spurge is classified as physiological dormancy that requires after-ripening and alternating temperature for maximal germination. Overlaps in transcriptome profiles between different phases of bud and seed dormancy have not been determined. Thus, we compared various phases of dormancy between seeds and buds to identify common genes and molecular processes, which should provide new insights about common regulators of dormancy. Results: Cluster analysis of expression profiles for 201 selected genes indicated bud and seed samples clustered separately. Direct comparisons between buds and seeds are additionally complicated since seeds incubated at a constant temperature of 20 degrees C for 21 days (21d C) could be considered paradormant (Para) because seeds may be inhibited by endosperm-generated signals, or ecodormant (Eco) because seeds germinate after being subjected to alternating temperature of 20:30 degrees C. Since direct comparisons in gene expression between buds and seeds were problematic, we instead examined commonalities in differentially-expressed genes associated with different phases of dormancy. Comparison between buds and seeds ('Para to Endo buds' and '21d C to 1d C seeds'), using endodormant buds (Endo) and dormant seeds (1d C) as common baselines, identified transcripts associated with cell cycle (HisH4), stress response/transcription factors (ICE2, ERFB4/ABR1), ABA and auxin response (ABA1, ARF1, IAA7, TFL1), carbohydrate/protein degradation (GAPDH_1), and transport (ABCB2). Comparison of transcript abundance for the 'Eco to Endo buds' and '21d C to 1d C seeds' identified transcripts associated with ABA response (ATEM6), auxin response (ARF1), and cell cycle (HisH4). These results indicate that the physiological state of 21d C seeds is more analogous to paradormant buds than that of ecodormant buds. Conclusion: Combined results indicate that common molecular mechanisms associated with dormancy transitions of buds and seeds involve processes associated with ABA and auxin signaling and transport, cell cycle, and AP2/ERF transcription factors or their up-stream regulators C1 [Chao, Wun S.; Dogramaci, Muenevver; Anderson, James V.; Foley, Michael E.; Horvath, David P.] USDA ARS, Biosci Res Lab, Sunflower & Plant Biol Res Unit, Fargo, ND 58102 USA. RP Chao, WS (reprint author), USDA ARS, Biosci Res Lab, Sunflower & Plant Biol Res Unit, 1605 Albrecht Blvd N, Fargo, ND 58102 USA. EM wun.chao@ars.usda.gov NR 38 TC 1 Z9 1 U1 1 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 12 PY 2014 VL 14 AR 216 DI 10.1186/s12870-014-0216-4 PG 9 WC Plant Sciences SC Plant Sciences GA AN9FP UT WOS:000340911900001 PM 25112962 ER PT J AU Irvin, MR Zhi, DG Joehanes, R Mendelson, M Aslibekyan, S Claas, SA Thibeault, KS Patel, N Day, K Jones, LW Liang, LM Chen, BH Yao, C Tiwari, HK Ordovas, JM Levy, D Absher, D Arnett, DK AF Irvin, Marguerite R. Zhi, Degui Joehanes, Roby Mendelson, Michael Aslibekyan, Stella Claas, Steven A. Thibeault, Krista S. Patel, Nikita Day, Kenneth Jones, Lindsay Waite Liang, Liming Chen, Brian H. Yao, Chen Tiwari, Hemant K. Ordovas, Jose M. Levy, Daniel Absher, Devin Arnett, Donna K. TI Epigenome-Wide Association Study of Fasting Blood Lipids in the Genetics of Lipid-Lowering Drugs and Diet Network Study SO CIRCULATION LA English DT Article DE cholesterol; fatty acids; genetics, medical; lipids; lipoproteins ID ACTIVATED RECEPTOR-ALPHA; CORONARY-HEART-DISEASE; FENOFIBRATE TREATMENT; INSULIN-RESISTANCE; MONONUCLEAR-CELLS; CPG SITES; GENOME; POLYMORPHISMS; PATTERNS; IDENTIFICATION AB Background-Genetic research regarding blood lipids has largely focused on DNA sequence variation; few studies have explored epigenetic effects. Genome-wide surveys of DNA methylation may uncover epigenetic factors influencing lipid metabolism. Methods and Results-To identify whether differential methylation of cytosine-(phosphate)-guanine dinucleotides (CpGs) correlated with lipid phenotypes, we isolated DNA from CD4+ T cells and quantified the proportion of sample methylation at >450 000 CpGs by using the Illumina Infinium HumanMethylation450 Beadchip in 991 participants of the Genetics of Lipid Lowering Drugs and Diet Network. We modeled the percentage of methylation at individual CpGs as a function of fasting very-low-density lipoprotein cholesterol and triglycerides (TGs) by using mixed linear regression adjusted for age, sex, study site, cell purity, and family structure. Four CpGs (cg00574958, cg17058475, cg01082498, and cg09737197) in intron 1 of carnitine palmitoyltransferase 1A (CPT1A) were strongly associated with very-low low-density lipoprotein cholesterol (P=1.8x10(-21) to 1.6x10(-8)) and TG (P=1.6x10(-26) to 1.5x10(-9)). Array findings were validated by bisulfite sequencing. We performed quantitative polymerase chain reaction experiments demonstrating that methylation of the top CpG (cg00574958) was correlated with CPT1A expression. The association of cg00574958 with TG and CPT1A expression were replicated in the Framingham Heart Study (P=4.1x10(-14) and 3.1x10(-13), respectively). DNA methylation at CPT1A cg00574958 explained 11.6% and 5.5% of the variation in TG in the discovery and replication cohorts, respectively. Conclusions-This genome-wide epigenomic study identified CPT1A methylation as strongly and robustly associated with fasting very-low low-density lipoprotein cholesterol and TG. Identifying novel epigenetic contributions to lipid traits may inform future efforts to identify new treatment targets and biomarkers of disease risk. C1 [Irvin, Marguerite R.; Aslibekyan, Stella; Claas, Steven A.; Arnett, Donna K.] Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, Birmingham, AL 35294 USA. [Zhi, Degui; Tiwari, Hemant K.] Univ Alabama Birmingham, Sect Stat Genet, Dept Biostat, Birmingham, AL 35294 USA. [Joehanes, Roby; Mendelson, Michael; Liang, Liming; Chen, Brian H.; Yao, Chen; Levy, Daniel] NHLBI, Populat Sci Branch, NIH, Bethesda, MD 20892 USA. [Joehanes, Roby; Mendelson, Michael; Liang, Liming; Chen, Brian H.; Yao, Chen; Levy, Daniel] Framingham Heart Dis Epidemiol Study, Framingham, MA USA. [Mendelson, Michael] Boston Childrens Hosp, Dept Cardiol, Boston, MA USA. [Thibeault, Krista S.; Patel, Nikita; Day, Kenneth; Jones, Lindsay Waite; Absher, Devin] Hudson Alpha Inst Biotechnol, Huntsville, AL USA. [Liang, Liming] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Liang, Liming] Harvard Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02115 USA. [Ordovas, Jose M.] Tufts Univ, Nutr & Genom Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Irvin, MR (reprint author), Univ Alabama Birmingham, Sch Publ Hlth, Dept Epidemiol, 1665 Univ Blvd,RPHB Room 220F, Birmingham, AL 35294 USA. EM irvinr@uab.edu RI Mendelson, Michael/I-2874-2014; OI Mendelson, Michael/0000-0001-7590-3958; Claas, Steven/0000-0001-9789-8395 FU National Institutes of Health (NIH)/National Heart, Lung, and Blood Institute (NHLBI) [R01 HL104135-01]; NIH/NHLBI [N01-HC-25195] FX The GOLDN epigenetics study is funded by National Institutes of Health (NIH)/National Heart, Lung, and Blood Institute (NHLBI) grant R01 HL104135-01. The Framingham Heart Study is supported by NIH/NHLBI contract N01-HC-25195. NR 50 TC 32 Z9 32 U1 0 U2 6 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0009-7322 EI 1524-4539 J9 CIRCULATION JI Circulation PD AUG 12 PY 2014 VL 130 IS 7 BP 565 EP + DI 10.1161/CIRCULATIONAHA.114.009158 PG 13 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AN6CJ UT WOS:000340681000013 PM 24920721 ER PT J AU Bin-Umer, MA McLaughlin, JE Butterly, MS McCormick, S Tumer, NE AF Bin-Umer, Mohamed Anwar McLaughlin, John E. Butterly, Matthew S. McCormick, Susan Tumer, Nilgun E. TI Elimination of damaged mitochondria through mitophagy reduces mitochondrial oxidative stress and increases tolerance to trichothecenes SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE Fusarium head blight; deoxynivalenol; Fusarium graminearum ID SACCHAROMYCES-CEREVISIAE; FUSARIUM-GRAMINEARUM; GENE-EXPRESSION; ARABIDOPSIS-THALIANA; UBIQUITIN PROTEASE; LIFE-SPAN; YEAST; MYCOTOXIN; MECHANISM; TOXICITY AB Trichothecene mycotoxins are natural contaminants of small grain cereals and are encountered in the environment, posing a worldwide threat to human and animal health. Their mechanism of toxicity is poorly understood, and little is known about cellular protection mechanisms against trichothecenes. We previously identified inhibition of mitochondrial protein synthesis as a novel mechanism for trichothecene-induced cell death. To identify cellular functions involved in trichothecene resistance, we screened the Saccharomyces cerevisiae deletion library for increased sensitivity to nonlethal concentrations of trichothecin (Tcin) and identified 121 strains exhibiting higher sensitivity than the parental strain. The largest group of sensitive strains had significantly higher reactive oxygen species (ROS) levels relative to the parental strain. A dose-dependent increase in ROS levels was observed in the parental strain treated with different trichothecenes, but not in a petite version of the parental strain or in the presence of a mitochondrial membrane uncoupler, indicating that mitochondria are the main site of ROS production due to toxin exposure. Cytotoxicity of trichothecenes was alleviated after treatment of the parental strain and highly sensitive mutants with antioxidants, suggesting that oxidative stress contributes to trichothecene sensitivity. Cotreatment with rapamycin and trichothecenes reduced ROS levels and cytotoxicity in the parental strain relative to the trichothecene treatment alone, but not in mitophagy deficient mutants, suggesting that elimination of trichothecene-damaged mitochondria by mitophagy improves cell survival. These results reveal that increased mitophagy is a cellular protection mechanism against trichothecene-induced mitochondrial oxidative stress and a potential target for trichothecene resistance. C1 [Bin-Umer, Mohamed Anwar; McLaughlin, John E.; Butterly, Matthew S.; Tumer, Nilgun E.] Rutgers State Univ, Sch Environm & Biol Sci, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. [McCormick, Susan] USDA ARS, Natl Ctr Agr Utilizat Res, Bacterial Foodborne Pathogens & Mycol Unit, Peoria, IL 61604 USA. RP Tumer, NE (reprint author), Rutgers State Univ, Sch Environm & Biol Sci, Dept Plant Biol & Pathol, New Brunswick, NJ 08901 USA. EM tumer@aesop.rutgers.edu FU US Department of Agriculture (USDA) [59-0206-1-121]; US Wheat and Barley Scab Initiative; New Jersey Agricultural Experiment Station; USDA-National Institute for Food and Agriculture, Hatch Project [NJ12117] FX We thank Dr. Benedikt Westermann for pVT100U-mtGFP, Dr. Jennifer Nielsen Kahn for determining the sensitivity of Vero cells to Tcin and for helpful comments, and Dr. Mike Pierce at the School of Environmental and Biological Sciences Core Facility for help with flow cytometry. This study is Cooperative Project 59-0206-1-121 supported by the US Department of Agriculture (USDA) in cooperation with the US Wheat and Barley Scab Initiative and by the New Jersey Agricultural Experiment Station and the USDA-National Institute for Food and Agriculture, Hatch Project Number NJ12117. NR 42 TC 12 Z9 13 U1 5 U2 29 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD AUG 12 PY 2014 VL 111 IS 32 BP 11798 EP 11803 DI 10.1073/pnas.1403145111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM8CQ UT WOS:000340097900055 PM 25071194 ER PT J AU Wu, QZ Prager, KC Goldstein, T Alt, DP Galloway, RL Zuerner, RL Lloyd-Smith, JO Schwacke, L AF Wu, Qingzhong Prager, Katherine C. Goldstein, Tracey Alt, David P. Galloway, Renee L. Zuerner, Richard L. Lloyd-Smith, James O. Schwacke, Lori TI Development of a real-time PCR for the detection of pathogenic Leptospira spp. in California sea lions SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Sea lions; Pathogenic Leptospira spp.; lipL32 gene; Real-time PCR; Urine; Kidney ID QUANTITATIVE PCR; IDENTIFICATION; GROWTH; POMONA AB Several real-time PCR assays are currently used for detection of pathogenic Leptospira spp.; however, few methods have been described for the successful evaluation of clinical urine samples. This study reports a rapid assay for the detection of pathogenic Leptospira spp. in California sea lions Zalophus californianus using real-time PCR with primers and a probe targeting the lipL32 gene. The PCR assay had high analytic sensitivity-the limit of detection was 3 genome copies per PCR volume using L. interrogans serovar Pomona DNA and 100% analytic specificity; it detected all pathogenic leptospiral serovars tested and none of the non-pathogenic Leptospira species (L. biflexa and L. meyeri serovar Semaranga), the intermediate species L. inadai, or the non-Leptospira pathogens tested. Our assay had an amplification efficiency of 1.00. Comparisons between the real-time PCR assay and culture isolation for detection of pathogenic Leptospira spp. in urine and kidney tissue samples from California sea lions showed that samples were more often positive by real-time PCR than by culture methods. Inclusion of an internal amplification control in the real-time PCR assay showed no inhibitory effects in PCR negative samples. These studies indicated that our real-time PCR assay has high analytic sensitivity and specificity for the rapid detection of pathogenic Leptospira species in urine and kidney tissue samples. C1 [Wu, Qingzhong; Schwacke, Lori] Natl Ocean Serv, Hollings Marine Lab, Natl Ctr Coastal Ocean Sci, NOAA, Charleston, SC 29412 USA. [Prager, Katherine C.; Lloyd-Smith, James O.] Univ Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA. [Prager, Katherine C.; Lloyd-Smith, James O.] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA. [Prager, Katherine C.] Marine Mammal Ctr, Sausalito, CA 94965 USA. [Goldstein, Tracey] Univ Calif Davis, Sch Vet Med, Hlth Inst 1, Davis, CA 95616 USA. [Alt, David P.; Zuerner, Richard L.] Natl Anim Dis Ctr, Infect Bacterial Dis Res Unit, Ames, IA 50010 USA. [Galloway, Renee L.] Ctr Dis Control & Prevent, Atlanta, GA 30333 USA. [Zuerner, Richard L.] USDA ARS, Washington, DC 20250 USA. RP Wu, QZ (reprint author), Natl Ocean Serv, Hollings Marine Lab, Natl Ctr Coastal Ocean Sci, NOAA, Charleston, SC 29412 USA. EM qingzhongwu@yahoo.com RI Lloyd-Smith, James/K-4080-2012 OI Lloyd-Smith, James/0000-0001-7941-502X FU NOAA's Oceans and Human Health Initiative; John H. Prescott Marine Mammal Rescue Assistance Grant Program; National Science Foundation [OCE-1335657]; RAPIDD program of the Science and Technology Directorate, Department of Homeland Security; Fogarty International Center, National Institutes of Health FX We thank Brian Thompson, for providing non-Leptospira bacteria. We also thank the staff of The Marine Mammal Center in Sausalito, California for providing the samples from the stranded sea lions; Dr. Robert DeLong and his group at the National Marine Mammal Laboratory for their collaboration and assistance in collecting samples from wild-caught, free-ranging sea lions. This work was performed in part at the University of California Natural Reserve System Ano Nuevo Island Reserve. We thank Ano Nuevo State Park rangers for their logistical support and Rick Hornsby at the National Animal Disease Center for his excellent technical support. Funding for this research was provided by NOAA's Oceans and Human Health Initiative, the John H. Prescott Marine Mammal Rescue Assistance Grant Program, and the National Science Foundation (OCE-1335657). K.C.P. and J.L.S. acknowledge financial support from the RAPIDD program of the Science and Technology Directorate, Department of Homeland Security and the Fogarty International Center, National Institutes of Health. This publication does not constitute an endorsement of any commercial product or intend to be an opinion beyond scientific or other results obtained by the NOAA or the ARS. NR 21 TC 1 Z9 1 U1 0 U2 8 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD AUG 11 PY 2014 VL 110 IS 3 BP 165 EP 172 DI 10.3354/dao02752 PG 8 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA AQ6LI UT WOS:000342923400001 PM 25114040 ER PT J AU Reshetnikov, AN Chestnut, T Brunner, JL Charles, K Nebergall, EE Olson, DH AF Reshetnikov, Andrey N. Chestnut, Tara Brunner, Jesse L. Charles, Kaylene Nebergall, Emily E. Olson, Deanna H. TI Detection of the emerging amphibian pathogens Batrachochytrium dendrobatidis and ranavirus in Russia SO DISEASES OF AQUATIC ORGANISMS LA English DT Article DE Amphibians; Chytridiomycosis; European common toad; Population fluctuations ID RANA-TEMPORARIA; BUFO-BUFO; CHYTRIDIOMYCOSIS; POPULATIONS; INFECTION; FISH; MORTALITY; DECLINES; DISEASE; SPAIN AB In a population of the European common toad Bufo bufo from a rural pond in the region of Lake Glubokoe Regional Reserve in Moscow province, Russia, unexplained mass mortality events involving larvae and metamorphs have been observed over a monitoring period of >20 yr. We tested toads from this and a nearby site for the emerging amphibian pathogens Batrachochytrium dendrobatidis (Bd) and ranavirus (Rv). Both pathogens were detected, and at the rural pond site, with the above-noted losses and decline in toad breeding success, 40% of B. bufo metamorphs were Bd positive, 46% were Rv positive and 20% were co-infected with both pathogens. Toad metamorphs from a neighbouring water body were also Bd and Rv positive (25 and 55%, respectively). This is the first confirmation of these pathogens in Russia. Questions remain as to the origins of these pathogens in Russia and their roles in documented mass mortality events. C1 [Reshetnikov, Andrey N.] Severtsov Ecol & Evolut Inst, Moscow 119071, Russia. [Chestnut, Tara] Oregon State Univ, Environm Sci Grad Program, Corvallis, OR 97331 USA. [Chestnut, Tara] US Geol Survey, Oregon Water Sci Ctr, Portland, OR 97201 USA. [Brunner, Jesse L.] Washington State Univ, Sch Biol Sci, Pullman, WA 99164 USA. [Charles, Kaylene] US Geol Survey, Natl Res Program, Reston, VA 20192 USA. [Nebergall, Emily E.] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA. [Olson, Deanna H.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Reshetnikov, AN (reprint author), Severtsov Ecol & Evolut Inst, Leninskiy 33, Moscow 119071, Russia. EM anreshetnikov@yandex.ru RI Brunner, Jesse/B-1692-2013 OI Brunner, Jesse/0000-0003-4894-8274 FU US Forest Service-Pacific Northwest Research Station; US Geological Survey Amphibian Research and Monitoring Initiative (ARMI) FX We thank K. Ronnenberg for assistance with figures. Support was provided by the US Forest Service-Pacific Northwest Research Station and the US Geological Survey Amphibian Research and Monitoring Initiative (ARMI). This manuscript is ARMI Contribution No. 473. This study was approved by the AN Severtsov Ecology & Evolution Institute Animal Ethics Committee (A2-2011). NR 32 TC 1 Z9 1 U1 0 U2 24 PU INTER-RESEARCH PI OLDENDORF LUHE PA NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY SN 0177-5103 EI 1616-1580 J9 DIS AQUAT ORGAN JI Dis. Aquat. Org. PD AUG 11 PY 2014 VL 110 IS 3 BP 235 EP 240 DI 10.3354/dao02757 PG 6 WC Fisheries; Veterinary Sciences SC Fisheries; Veterinary Sciences GA AQ6LI UT WOS:000342923400008 PM 25114047 ER PT J AU Root, JJ Shriner, SA Bentler, KT Gidlewski, T Mooers, NL Spraker, TR VanDalen, KK Sullivan, HJ Franklin, AB AF Root, J. Jeffrey Shriner, Susan A. Bentler, Kevin T. Gidlewski, Thomas Mooers, Nicole L. Spraker, Terry R. VanDalen, Kaci K. Sullivan, Heather J. Franklin, Alan B. TI Shedding of a Low Pathogenic Avian Influenza Virus in a Common Synanthropic Mammal - The Cottontail Rabbit SO PLOS ONE LA English DT Article ID UNITED-STATES; INFECTION; SUBTYPES; ASSAY AB Background: Cottontails (Sylvilagus spp.) are common mammals throughout much of the U. S. and are often found in peridomestic settings, potentially interacting with livestock and poultry operations. If these animals are susceptible to avian influenza virus (AIV) infections and shed the virus in sufficient quantities they may pose a risk for movement of avian influenza viruses between wildlife and domestic animals in certain situations. Methodology/Principal Findings: To assess the viral shedding potential of AIV in cottontails, we nasally inoculated fourteen cottontails with a low pathogenic AIV (H4N6). All inoculated cottontails shed relatively large quantities of viral RNA both nasally (<= 10(6.94) PCR EID50 equivalents/mL) and orally (<= 10(5.09) PCR EID50 equivalents/mL). However, oral shedding tended to decline more quickly than did nasal shedding. No animals showed any obvious signs of disease throughout the study. Evidence of a serological response was found in all infected rabbits at 22 days post infection in convalescent sera. Conclusions/Significance: To our knowledge, cottontails have not been previously assessed for AIV shedding. However, it was obvious that they shed AIV RNA extensively via the nasal and oral routes. This is significant, as cottontails are widely distributed throughout the U. S. and elsewhere. These mammals are often found in highly peridomestic situations, such as farms, parks, and suburban neighborhoods, often becoming habituated to human activities. Thus, if infected these mammals could easily transport AIVs short distances. C1 [Root, J. Jeffrey; Shriner, Susan A.; Bentler, Kevin T.; Gidlewski, Thomas; Mooers, Nicole L.; VanDalen, Kaci K.; Sullivan, Heather J.; Franklin, Alan B.] US Fish & Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80525 USA. [Spraker, Terry R.] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA. RP Root, JJ (reprint author), US Fish & Wildlife Serv, USDA, Natl Wildlife Res Ctr, Ft Collins, CO 80525 USA. EM Jeff.root@aphis.usda.gov OI Shriner, Susan/0000-0003-0349-7182 FU United States Department of Agriculture FX This study was funded by the United States Department of Agriculture. The manuscript was reviewed for general policy statements committing the USDA to action, but otherwise the findings were independently developed by the authors. NR 32 TC 3 Z9 3 U1 1 U2 6 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 11 PY 2014 VL 9 IS 8 AR e102513 DI 10.1371/journal.pone.0102513 PG 5 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AO1WB UT WOS:000341105100009 PM 25111780 ER PT J AU Adidepe, O Johanningsmeier, SD Yencho, C Truong, VD AF Adidepe, Oluwatosin Johanningsmeier, Suzanne D. Yencho, Craig Truong, Van-Den TI Development of a near infrared (NIR) spectroscopy model for prediction of acrylamide content in french-fried potatoes SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Adidepe, Oluwatosin; Yencho, Craig] N Carolina State Univ, Dept Hort, Raleigh, NC 27695 USA. [Johanningsmeier, Suzanne D.; Truong, Van-Den] ARS, Food Sci Res Unit, USDA, Raleigh, NC 27695 USA. [Johanningsmeier, Suzanne D.; Truong, Van-Den] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA. EM oeadedip@ncsu.edu NR 0 TC 0 Z9 0 U1 3 U2 10 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 207-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100278 ER PT J AU Agramonte, NM Bloomquist, JR Bernier, UR AF Agramonte, Natasha M. Bloomquist, Jeffrey R. Bernier, Ulrich R. TI Resistance of Puerto Rican Aedes aegypti to permethrin, etofenprox, and propoxur by LD50 estimation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Agramonte, Natasha M.; Bloomquist, Jeffrey R.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32608 USA. [Agramonte, Natasha M.; Bernier, Ulrich R.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM nme@ufl.edu NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 57-TOXI PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167406214 ER PT J AU Albright, VC Hellmich, RL Coats, JR AF Albright, Vurtice C. Hellmich, Richard L. Coats, Joel R. TI ELISAs and Cry proteins: What are we really detecting? SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Albright, Vurtice C.; Hellmich, Richard L.; Coats, Joel R.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Hellmich, Richard L.] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA 50011 USA. EM valbrigh@iastate.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 444-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100803 ER PT J AU Albright, VC Hellmich, RL Coats, JR AF Albright, Vurtice C. Hellmich, Richard L. Coats, Joel R. TI Biological validation of enzyme-linked immunosorbent assays for detection of Cry proteins in the environment SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Albright, Vurtice C.; Hellmich, Richard L.; Coats, Joel R.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Hellmich, Richard L.] ARS, Corn Insects & Crop Genet Res Unit, USDA, Ames, IA 50011 USA. EM valbrigh@iastate.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 306-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100671 ER PT J AU Andrade, NA Centofanti, T McConnell, LL Hapeman, CJ Torrents, A Nguyen, A Beyer, WN Chaney, RL Novak, JM Anderson, MO Cantrell, KB AF Andrade, Natasha A. Centofanti, Tiziana McConnell, Laura L. Hapeman, Cathleen J. Torrents, Alba Anh Nguyen Beyer, W. Nelson Chaney, Rufus L. Novak, Jeffrey M. Anderson, Marya O. Cantrell, Keri B. TI NEW INVESTIGATOR AWARD FINALIST: Utilizing thin-film solid-phase extraction to assess the effect of organic carbon amendments on the bioavailability of DDT and dieldrin to earthworms SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Andrade, Natasha A.; Centofanti, Tiziana; McConnell, Laura L.; Torrents, Alba; Anderson, Marya O.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Hapeman, Cathleen J.; Anh Nguyen; Chaney, Rufus L.] ARS, USDA, Beltsville, MD 20705 USA. [Beyer, W. Nelson] US Geol Survey, Patuxent Wildlife Res Ctr, Beltsville, MD 20705 USA. [Novak, Jeffrey M.; Cantrell, Keri B.] ARS, USDA, Florence, SC 29501 USA. EM nandrade@umd.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 351-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100715 ER PT J AU Bai, JH Baldwin, E Plotto, A Cameron, R Luzio, G Ford, B Manthey, J AF Bai, Jinhe Baldwin, Elizabeth Plotto, Anne Cameron, Randall Luzio, Gary Ford, Bryan Manthey, John TI Effect of production methods on flavor, cloud stability, and other quality attributes of orange juice SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Bai, Jinhe; Baldwin, Elizabeth; Plotto, Anne; Cameron, Randall; Luzio, Gary; Ford, Bryan; Manthey, John] ARS, HRL, USDA, Ft Pierce, FL 34945 USA. EM jinhe.bai@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 149-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100221 ER PT J AU Beck, JJ AF Beck, John J. TI Using a plant-insect-fungus relationship to develop a sustainable host plant volatile-based attractant for a major agricultural insect pest SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Beck, John J.] ARS, USDA, Albany, CA USA. EM john.beck@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 37-IAC PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167400555 ER PT J AU Beck, JJ AF Beck, John J. TI An old approach to a new discovery of an effective host plant volatile-based attractant for a major insect pest SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Beck, John J.] ARS, USDA, Albany, CA 94710 USA. EM john.beck@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 42-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100415 ER PT J AU Bernier, UR Agramonte, NM Perry, MK Johnson, AL AF Bernier, Ulrich R. Agramonte, Natasha M. Perry, Melynda K. Johnson, Amy L. TI Evaluation of permethrin-treated US military combat uniforms SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Bernier, Ulrich R.] ARS, USDA, CMAVE, Gainesville, FL 32608 USA. [Agramonte, Natasha M.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Perry, Melynda K.; Johnson, Amy L.] Natick Soldier Res Dev & Engn Ctr, Natick, MA 01760 USA. EM uli.bernier@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 349-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100713 ER PT J AU Boulange, J Watanabe, H Zhang, MH Luo, YZ Arnold, J AF Boulange, Julien Watanabe, Hirozumi Zhang, Minghua Luo, Yuzhou Arnold, Jeff TI Framework development of rice pesticide modeling in the Colusa Drain basin, California SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Boulange, Julien; Watanabe, Hirozumi] Tokyo Univ Agr & Technol, Grad Sch Agr, Fuchu, Tokyo, Japan. [Zhang, Minghua; Luo, Yuzhou] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Arnold, Jeff] ARS, USDA, Temple, TX USA. EM pochi@cc.tuat.ac.jp RI Watanabe, Hirozumi/G-1071-2013 OI Watanabe, Hirozumi/0000-0002-5658-8822 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 505-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100858 ER PT J AU Brandon, DL Korn, AM AF Brandon, David L. Korn, Anna M. TI Immunosorbent analysis of ricin and Shiga toxin contamination in milk and ground beef using IgY-based ELISA with colorimetric or chemiluminescent detection SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Brandon, David L.; Korn, Anna M.] ARS, Foodborne Toxin Detect & Prevent Res Unit, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. EM david.brandon@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 212-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100283 ER PT J AU Braverman, MP Kunkel, D Baron, J Sandahl, J AF Braverman, Michael P. Kunkel, Daniel Baron, Jerry Sandahl, Jason TI Challenges of considering harmonization of GAP and trade in protocol development for magnitude of residue studies in ASEAN and African countries SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Braverman, Michael P.; Kunkel, Daniel; Baron, Jerry] Rutgers State Univ, IR Project 4, Princeton, NJ 08540 USA. [Sandahl, Jason] Foreign Agr Serv, USDA, Washington, DC USA. EM braverman@aesop.rutgers.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 581-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101047 ER PT J AU Cameron, RG Grohman, K Kim, Y Galant, A Widmer, W Luzio, G AF Cameron, Randall G. Grohman, Karel Kim, Yang Galant, Ashley Widmer, Wilbur Luzio, Gary TI Extraction, recovery, and functionality of pectic hydrocolloids from citrus processing waste SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Cameron, Randall G.; Widmer, Wilbur; Luzio, Gary] ARS, USDA, Ft Pierce, FL 34945 USA. [Grohman, Karel] Renewable Spirits LLC, Delray Beach, FL USA. [Kim, Yang] Sejong Univ, Dept Food Sci & Technol, Seoul, South Korea. [Galant, Ashley] ARS, USDA, Manhattan, KS USA. EM randall.cameron@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 296-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100361 ER PT J AU Chambers, AS AF Chambers, Adam S. TI Agricultural conservation: Tools for mitigating and adapting to our changing climate SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Chambers, Adam S.] Nat Resources Conservat Serv, Air Qual & Atmospher Change Team, USDA, Portland, OR 97232 USA. EM adam.chambers@por.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 439-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100798 ER PT J AU Chang, SC Nguyen, TM Condon, B AF Chang, SeChin Thach-Mien Nguyen Condon, Brian TI Studies of flammability and thermal degradation for flame retardant cotton fabric with P-N containing derivatives SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Chang, SeChin; Thach-Mien Nguyen; Condon, Brian] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM sechin.chang@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 43-CARB PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165102206 ER PT J AU Chauhan, K AF Chauhan, Kamal TI Insecticides of plant origin: Biorational synthesis SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Chauhan, Kamal] ARS, Invas Insect Biocontrol & Behav Lab, USDA, Beltsville, MD 20705 USA. EM kamal.chauhan@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 346-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100710 ER PT J AU Chen, J Park, B Huang, YW Zhao, YP AF Chen, Jing Park, Bosoon Huang, Yao-wen Zhao, Yiping TI Label-free SERS detection of Salmonella Typhimurium on aptamer modified AgNR substrates SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Chen, Jing; Huang, Yao-wen] Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA. [Zhao, Yiping] Univ Georgia, Dept Phys & Astron, Athens, GA 30602 USA. [Park, Bosoon] ARS, Russell Res Ctr, USDA, Athens, GA 30605 USA. [Chen, Jing; Zhao, Yiping] Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA. EM bosoon.park@ars.usda.gov RI Zhao, Yiping/A-4968-2008 NR 0 TC 0 Z9 0 U1 3 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 139-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100211 ER PT J AU Cheng, HN Biswas, A AF Cheng, H. N. Biswas, Atanu TI Green-chemistry synthesis of cellulosic derivatives SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Cheng, H. N.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Biswas, Atanu] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM hn.cheng@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 679-POLY PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167405789 ER PT J AU Compton, DL Laszlo, JA Appell, M Evans, KO AF Compton, David L. Laszlo, Joseph A. Appell, Michael Evans, Kervin O. TI Synthesis, isolation, and acyl migration kinetics of desaturated and hydroxylated 2-monoacylglycerols SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Compton, David L.; Laszlo, Joseph A.; Appell, Michael; Evans, Kervin O.] USDA, Washington, DC USA. EM david.compton@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 292-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100357 ER PT J AU Cordoba, CT Koskinen, WC Kookana, R AF Cordoba, Carmen Trigo Koskinen, William C. Kookana, Rai TI Sorption-desorption of indaziflam and its three metabolites in sandy soils SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Cordoba, Carmen Trigo] Univ Minnesota, St Paul, MN 55108 USA. [Koskinen, William C.] ARS, USDA, St Paul, MN 55108 USA. [Kookana, Rai] CSIRO, Glen Osmond, SA, Australia. EM carmentrigo1@hotmail.com NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 376-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100738 ER PT J AU Cote, GL Skory, CD Cormier, RS AF Cote, Gregory L. Skory, Christopher D. Cormier, Ryan S. TI Custom-tailored water-insoluble glucans from sucrose via glucansucrases SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Cote, Gregory L.; Skory, Christopher D.; Cormier, Ryan S.] ARS, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM greg.cote@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 36-POLY PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167405172 ER PT J AU DeGrandi-Hoffman, G Chen, YP Watkins-DeJong, E Chambers, ML Hidalgo, G AF DeGrandi-Hoffman, Gloria Chen, Yanping (Judy) Watkins-DeJong, Emily Chambers, Mona L. Hidalgo, Geoffrey TI Effects of boscalid and pyraclostrobin on ATP concentrations, protein digestion, and virus titers in honey bees (Apis mellifera L.) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [DeGrandi-Hoffman, Gloria; Watkins-DeJong, Emily; Chambers, Mona L.; Hidalgo, Geoffrey] ARS, USDA, Carl Hayden Bee Res Ctr, Tucson, AZ 85719 USA. [Chen, Yanping (Judy)] ARS, USDA, Beltsville Bee Lab, Beltsville, MD 20705 USA. EM Gloria.Hoffman@ARS.USDA.GOV NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 601-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101067 ER PT J AU Derksen, RC Ozkan, E Paul, PA Zhu, HP AF Derksen, Richard C. Ozkan, Erdal Paul, Pierce A. Zhu, Heping TI Spray deposition field studies and implications for label guidelines SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Derksen, Richard C.; Zhu, Heping] ARS, USDA, Wooster, OH 44691 USA. [Ozkan, Erdal] Ohio State Univ, Columbus, OH 43210 USA. [Paul, Pierce A.] Ohio State Univ, Dept Plant Pathol, Wooster, OH 44691 USA. EM rich.derksen@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 666-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101128 ER PT J AU Dickens, JC AF Dickens, Joseph C. TI Differential detection of odorous enantiomers by insects SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Dickens, Joseph C.] ARS, Invas Insect Biocontrol & Behav Lab, USDA, BARC, Beltsville, MD 20705 USA. EM joseph.dickens@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 152-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100224 ER PT J AU Duke, SO AF Duke, Stephen O. TI Greener chemistry for pest management SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Duke, Stephen O.] ARS, USDA, University, MS 38677 USA. EM sduke@olemiss.edu NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 39-IAC PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167400557 ER PT J AU Duke, SO AF Duke, Stephen O. TI Discovery of new herbicide modes of action with natural phytotoxins SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Duke, Stephen O.] ARS, USDA, Oxford, MS 38655 USA. EM stephen.duke@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 184-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100549 ER PT J AU Evans, KO Laszlo, JA Compton, DL AF Evans, Kervin O. Laszlo, Joseph A. Compton, David L. TI Bilayer properties of hydroxytyrosol- and tyrosol-phosphatidylcholine lipids SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Evans, Kervin O.; Laszlo, Joseph A.; Compton, David L.] ARS, USDA, Peoria, IL 61607 USA. EM Kervin.Evans@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 229-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100299 ER PT J AU Fortin, MS Beromi, MM Lai, A Mullen, CA West, NM AF Fortin, Michael S. Beromi, Megan Mohedjer Lai, Amy Mullen, Charles A. West, Nathan M. TI Transition metal catalyzed lignin oxidative cleavage SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Fortin, Michael S.; Beromi, Megan Mohedjer; Lai, Amy; West, Nathan M.] Allegheny Univ Hlth Sci, Dept Chem & Biochem, Philadelphia, PA 19104 USA. [Mullen, Charles A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM mfortin@mail.usciences.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 710-INOR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167401359 ER PT J AU Gao, BY Lu, YJ Chen, P Yu, LL AF Gao, Boyan Lu, Yingjian Chen, Pei Yu, Liangli (Lucy) TI Differentiating organic and conventional sage by chromatographic and mass spectrometry flow injection fingerprints combined with principal component analysis SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Gao, Boyan; Lu, Yingjian; Yu, Liangli (Lucy)] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Gao, Boyan; Yu, Liangli (Lucy)] Shanghai Jiao Tong Univ, Inst Food & Nutraceut Sci, Sch Agr & Biol, Shanghai 200240, Shanghai, Peoples R China. [Chen, Pei] ARS, Food Composit Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM raphaelgao1985@gmail.com RI Gao, Boyan/N-9851-2016 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 195-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100266 ER PT J AU Geis-Asteggiante, L McConnell, LL Hapeman, CJ McCarty, GW Rice, CP Nguyen, A Downey, P Torrents, A AF Geis-Asteggiante, Lucia McConnell, Laura L. Hapeman, Cathleen J. McCarty, Gregory W. Rice, Clifford P. Nguyen, Anh Downey, Peter Torrents, Alba TI Developing unique tracers to distinguish nutrient contributions from agriculture and wastewater sources in the Choptank River and Anacostia River watersheds SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Geis-Asteggiante, Lucia] Univ Maryland, College Pk, MD 20742 USA. [McConnell, Laura L.] Bayer CropSci, Res Triangle Pk, NC 27709 USA. [Hapeman, Cathleen J.; McCarty, Gregory W.; Rice, Clifford P.; Nguyen, Anh; Downey, Peter; Torrents, Alba] USDA ARS, Beltsville, MD 20705 USA. EM cathleen.hapeman@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 480-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100835 ER PT J AU Goebel, T Davis, T Lascano, R AF Goebel, Timothy Davis, Todd Lascano, Robert TI Evaluation of several commercially available cationic polymer flocculants for phosphate sorption in water SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Goebel, Timothy; Lascano, Robert] USDA ARS, Lubbock, TX 79415 USA. [Davis, Todd] Idaho State Univ, Pocatello, ID 83209 USA. EM tim.goebel@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 77-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100450 ER PT J AU Goldade, DA Dorr, BS Clark, CS AF Goldade, David A. Dorr, Brian S. Clark, Craig S. TI Toxin metabolism in the invasive brown treesnake (Boiga irregularis) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Goldade, David A.] USDA WS Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Dorr, Brian S.] USDA WS Natl Wildlife Res Ctr, Starkville, MS 39762 USA. [Clark, Craig S.] USDA Wildlife Serv Hawaii Guam Pacific Isl, Barrigada, GU 96913 USA. EM David.A.Goldade@aphis.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 75-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165105513 ER PT J AU Curbelo, MAG Lehotay, SJ Borges, JH Delgado, MAR AF Gonzalez Curbelo, Miguel Angel Lehotay, Steven J. Hernandez Borges, Javier Rodriguez Delgado, Miguel Angel TI Ammonium formate buffer in QuEChERS for high throughput analysis of pesticides in food by fast, low-pressure GC-MS/MS and LC-MS/MS SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Gonzalez Curbelo, Miguel Angel; Lehotay, Steven J.] ARS, USDA, Wyndmoor, PA 19038 USA. [Gonzalez Curbelo, Miguel Angel; Hernandez Borges, Javier; Rodriguez Delgado, Miguel Angel] Univ La Laguna, Dept Chem, San Cristobal la Laguna 38206, Canary Islands, Spain. EM maglezc@ull.es RI Hernandez-Borges, Javier/B-7624-2011; RODRIGUEZ-DELGADO, MIGUEL ANGEL/J-9719-2012 OI Hernandez-Borges, Javier/0000-0001-8895-5554; NR 1 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 259-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100624 ER PT J AU Gonzalez, JM Smith, D Livingston, S Francesconi, W AF Gonzalez, Javier M. Smith, Douglas Livingston, Stanley Francesconi, Wendy TI Atrazine and simazine transport and persistence in soils in corn-soybean rotations SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Gonzalez, Javier M.; Smith, Douglas; Livingston, Stanley; Francesconi, Wendy] ARS, USDA, NSERL, W Lafayette, IN 47907 USA. EM javier.gonzalez@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 487-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100842 ER PT J AU Gross, AD Kimber, MJ Temeyer, KB Miller, RJ de Leon, AAP Coats, JR AF Gross, Aaron D. Kimber, Michael J. Temeyer, Kevin B. Miller, Robert J. de Leon, Adalberto A. Perez Coats, Joel R. TI Tyramine receptor from the southern cattle tick: A potential target for plant terpenoids SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Gross, Aaron D.; Coats, Joel R.] Iowa State Univ Sci & Technol, Dept Entomol, Ames, IA 50011 USA. [Gross, Aaron D.; Kimber, Michael J.] Iowa State Univ Sci & Technol, Dept Biomed Sci, Ames, IA 50011 USA. [Temeyer, Kevin B.; de Leon, Adalberto A. Perez] ARS, Knipling Bushland US Livestock Insect Res Lab, USDA, Kerrville, TX 78029 USA. [Miller, Robert J.] ARS, Cattle Fever Tick Res Lab, USDA, Edinburg, TX 78541 USA. EM adgross@gmail.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 673-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101135 ER PT J AU Hall, KE Calderon, MJ Spokas, KA Cox, L Koskinen, WC Novak, J Cantrell, K AF Hall, Kathleen E. Calderon, Maria J. Spokas, Kurt A. Cox, Lucia Koskinen, William C. Novak, Jeffrey Cantrell, Keri TI Effect of biochar on the fate and behavior of allelochemicals in soil SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Hall, Kathleen E.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Calderon, Maria J.; Cox, Lucia] CSIC IRNAS, Madrid, Spain. [Spokas, Kurt A.; Koskinen, William C.; Novak, Jeffrey; Cantrell, Keri] ARS, USDA, Washington, DC USA. EM hall0995@umn.edu RI COX, LUCIA/M-4072-2015; Spokas, Kurt/F-4839-2016 OI Spokas, Kurt/0000-0002-5049-5959 NR 0 TC 0 Z9 0 U1 0 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 375-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100737 ER PT J AU Hall, WA Walse, SS AF Hall, Wiley A. Walse, Spencer S. TI Methyl bromide release from activated carbon and the soil/water/carbon interface SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Hall, Wiley A.; Walse, Spencer S.] ARS, USDA, Parlier, CA 93648 USA. EM wiley.hall@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 151-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165105585 ER PT J AU Hellmich, RL AF Hellmich, Richard L. TI A tale of two insects: Managing insect resistance to genetically-engineered crops SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Hellmich, Richard L.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Hellmich, Richard L.] Iowa State Univ, USDA ARS, Dept Entomol, Ames, IA 50011 USA. EM Richard.Hellmich@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 618-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101083 ER PT J AU Hunter, WB Andrade, EC AF Hunter, Wayne B. Andrade, Eduardo C. TI Challenges and considerations for RNAi-based technologies in insect management (hemipterans: aphids, leafhoppers, psyllids) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Hunter, Wayne B.; Andrade, Eduardo C.] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. EM wayne.hunter@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 11 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 150-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100518 ER PT J AU Jackson, MA Appell, M Blackburn, JA AF Jackson, Michael A. Appell, Michael Blackburn, Judith A. TI Fructose hydrogenation to 2,5-dimethyltetrahydrofuran over a sulfur-poisoned pt/c catalyst SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Jackson, Michael A.; Appell, Michael; Blackburn, Judith A.] USDA, Washington, DC USA. EM michael.jackson@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 293-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100358 ER PT J AU Jong, L AF Jong, Lei TI Interface interactions of natural rubber and protein/fiber aggregates SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Jong, Lei] USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 313-PMSE PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167404678 ER PT J AU Karaarslan, T Qian, YPL Yorgey, B Finn, C Qian, M AF Karaarslan, Tuba Qian, Yanping L. Yorgey, Brian Finn, Chad Qian, Michael TI Isomeric ratio of linalool and a-terpineol in high bush blueberry fruit SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Karaarslan, Tuba; Qian, Yanping L.; Yorgey, Brian; Qian, Michael] Oregon State Univ, Corvallis, OR 97330 USA. [Finn, Chad] ARS, Northwest Ctr Small Fruit Res, USDA, Corvallis, OR 97331 USA. EM Tuba.Karaarslan@oregonstate.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 227-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100297 ER PT J AU Kim, H Nah, SY Seo, KH Yokoyama, W AF Kim, Hyunsook Nah, Seung-Yeol Seo, Kun-Ho Yokoyama, Wallace TI Gintonin, a novel glycoprotein extracted from Panax ginseng, improves cognitive function and reduces fat accumulation in adipocytes SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Kim, Hyunsook; Nah, Seung-Yeol] Konkuk Univ, Dept Physiol, Coll Vet Med, Seoul, South Korea. [Seo, Kun-Ho] Konkuk Univ, Coll Vet Med, Dept Publ Hlth, Konkuk, South Korea. [Yokoyama, Wallace] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM hyskim@ucdavis.edu NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 112-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100184 ER PT J AU Kim, JH Chan, KL Stanker, LH AF Kim, Jong H. Chan, Kathleen L. Stanker, Larry H. TI Management of fungicide resistance, mycotoxin production, and food safety risks by modulating molecular targets in fungal pathogens SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Kim, Jong H.; Chan, Kathleen L.; Stanker, Larry H.] ARS, Western Reg Res Ctr, Foodborne Toxin Detect & Prevent Res Unit, USDA, Albany, CA 94710 USA. EM jongheon.kim@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 995-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101444 ER PT J AU Kim, S AF Kim, Sanghoon TI Utilization of protein-based nanoparticles developed for hydrophilic coating as an interface modifier in the latex elastomer SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Kim, Sanghoon] USDA ARS NCAUR, Peoria, IL 61604 USA. EM sanghoon.kim@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 165-COLL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165103606 ER PT J AU Li, AY Costa, LM Chauhan, KR Coats, JR Cantrell, CL Temeyer, KB de Leon, AAP AF Li, Andrew Y. Costa-Junior, Livio M. Chauhan, Kamal R. Coats, Joel R. Cantrell, Charles L. Temeyer, Kevin B. de Leon, Adalberto A. Perez TI Evaluation of essential oils as natural pesticides and repellents against ticks and biting flies affecting livestock and human health SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Li, Andrew Y.; Chauhan, Kamal R.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. [Costa-Junior, Livio M.] Univ Fed Maranhao, Ctr Agr & Environm Sci, BR-65500000 Chapadinha, Maranhao, Brazil. [Coats, Joel R.] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Cantrell, Charles L.] USDA ARS, Nat Prod Utilizat Res Lab, University, MS 38677 USA. [Temeyer, Kevin B.; de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. EM Andrew.Li@ars.usda.gov RI Costa-Junior, Livio/H-7530-2012 OI Costa-Junior, Livio/0000-0002-1475-049X NR 0 TC 0 Z9 0 U1 0 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 456-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100815 ER PT J AU Lovanh, N Loughrin, J Quintanar, A Ryzs, M Mahmood, R Oh, BT AF Lovanh, Nanh Loughrin, John Quintanar, Arturo Ryzs, Maciej Mahmood, Rezual Oh, Byung-Taek TI Modeling the effect of heat fluxes on ammonia and nitrous oxide emissions from an anaerobic swine waste treatment lagoon using artificial neural network SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Lovanh, Nanh; Loughrin, John] ARS, USDA, Bowling Green, KY 42104 USA. [Quintanar, Arturo; Mahmood, Rezual] Western Kentucky Univ, Bowling Green, KY 42104 USA. [Ryzs, Maciej] Univ Iowa, Iowa City, IA 52240 USA. [Oh, Byung-Taek] Chonbuk Natl Univ, Iksan 570752, Jeonbuk, South Korea. EM nanh.lovanh@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 241-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100310 ER PT J AU Luthria, D AF Luthria, Devanand TI Effect of processing on phytochemical composition of flour, dough, and bread fractions made from refined and whole wheat SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Luthria, Devanand] USDA, Food Composit Methods Dev Lab, Laurel, MD 20723 USA. EM Dave.Luthria@gmail.com NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 300-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100365 ER PT J AU Maleki, SJ AF Maleki, Soheila J. TI Effects of thermal processing on peanut allergens SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Maleki, Soheila J.] ARS, Dept Food Allergy Res, USDA, New Orleans, LA 70124 USA. EM soheila.maleki@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 63-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100135 ER PT J AU Manthey, JA Bai, JH Raithore, S Baldwin, EA Cesar, TB Silveira, J AF Manthey, John A. Bai, Jinhe Raithore, Smita Baldwin, Elizabeth A. Cesar, Thais B. Silveira, Jacqueline TI Effects of juice production methods on flavonoids and other secondary metabolites in fresh and processed orange juice SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Manthey, John A.; Bai, Jinhe; Raithore, Smita; Baldwin, Elizabeth A.] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Cesar, Thais B.; Silveira, Jacqueline] Sao Paulo State Univ, Dept Pharmaceut Sci, Araraquara, Brazil. EM john.manthey@ars.usda.gov RI Cesar, Thais/I-4540-2013 OI Cesar, Thais/0000-0001-7878-7075 NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 173-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100244 ER PT J AU Mattison, CP Desormeaux, WA Wasserman, RL Yoshioka-Tarver, M Condon, B Grimm, CC AF Mattison, Chris P. Desormeaux, Wendy A. Wasserman, Richard L. Yoshioka-Tarver, Megumi Condon, Brian Grimm, Casey C. TI Processing routes to reduce IgE binding to cashew allergens SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Mattison, Chris P.; Desormeaux, Wendy A.; Condon, Brian; Grimm, Casey C.] ARS, USDA, New Orleans, LA 70471 USA. [Wasserman, Richard L.] Univ Texas Southwestern Med Sch, Allergy Immunol Res Ctr North Texas, Dept Pediat, Dallas, TX 75390 USA. [Yoshioka-Tarver, Megumi] Southern Univ & A&M Coll, Baton Rouge, LA 70813 USA. EM chris.mattison@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 86-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100158 ER PT J AU Mbughuni, MM Matt, F Book, A Hunt, C Currie, C Fox, BG AF Mbughuni, Michael M. Matt, Frederick Book, Adam Hunt, Christopher Currie, Cameron Fox, Brian G. TI Kinetic and mechanistic studies with polysaccharide monooxygenase from SirexAA-E SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Mbughuni, Michael M.; Fox, Brian G.] Univ Wisconsin, Deartment Biochem, Madison, WI 53706 USA. [Book, Adam; Currie, Cameron] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Matt, Frederick; Hunt, Christopher] US Forest Serv, Forest Prod Lab Madison, USDA, Madison, WI 53726 USA. EM mbughuni@wisc.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 46-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101857 ER PT J AU Miri, MJ Kim, S Kudla, AM Allston, TD Glenn, GM Klamczynski, A AF Miri, Massoud J. Kim, Seohyun Kudla, Amber M. Allston, Thomas D. Glenn, Gregory M. Klamczynski, Artur TI Dioxolanes of 2-heptanone as mite repellants for the protection of bees SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Miri, Massoud J.; Kim, Seohyun; Kudla, Amber M.; Allston, Thomas D.] Rochester Inst Technol, Sch Chem & Mat Sci, Rochester, NY 14623 USA. [Glenn, Gregory M.; Klamczynski, Artur] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. EM mjmsch@rit.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 301-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100366 ER PT J AU O'Keefe, M Bennett, P Muniz-Ortiz, J AF O'Keefe, Margaret Bennett, Patricia Muniz-Ortiz, Jorge TI Scanning the horizon through information-based hazard identification: New advances and opportunities to get involved SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [O'Keefe, Margaret; Bennett, Patricia; Muniz-Ortiz, Jorge] USDA, Fed Govt, Washington, DC 20250 USA. EM margaret.okeefe@fsis.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 208-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100279 ER PT J AU Orts, W AF Orts, William TI Strategies for creating sustainable chemicals and biofuels from agriculturally derived feedstocks: AGFD perspective SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Orts, William] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. EM bill.orts@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 11-CHED PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165102663 ER PT J AU Papiernik, SK Koskinen, WC Yates, SR AF Papiernik, Sharon K. Koskinen, William C. Yates, Scott R. TI Exploring linkages between time-dependent sorption and biodegradation SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Papiernik, Sharon K.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Koskinen, William C.] USDA ARS, Soil & Water Management Res Unit, St Paul, MN 55108 USA. [Yates, Scott R.] USDA ARS, US Salin Lab, Riverside, CA 92507 USA. EM sharon.papiernik@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 474-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100831 ER PT J AU Parish, EJ Lin, JT Wei, TY Honda, H AF Parish, Edward J. Lin, Jiann-Tsyh Wei, Tsao-Yi Honda, Hiroshi TI Application of scanning electron microscopes and the comparison of non-native tephritid flies SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Parish, Edward J.] Auburn Univ, Dept Chem & Biochem, Auburn, AL 36849 USA. [Lin, Jiann-Tsyh] USDA, Dept Crop Improvement & Utilizat Res, Albany, CA 94710 USA. [Wei, Tsao-Yi; Honda, Hiroshi] Northwestern Polytech Univ, Dept Bioengn, Fremont, CA 94539 USA. EM chrishonda@yahoo.com NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 115-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165102026 ER PT J AU Perry, CH Seilheimer, TS Zimmerman, PL Stueve, KM AF Perry, Charles H. Seilheimer, Titus S. Zimmerman, Patrick L. Stueve, Kirk M. TI Terrestrial sources of phosphorus and turbidity to Lake Superior and Lake Michigan can be identified using forest disturbance and landscape metrics SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Perry, Charles H.; Seilheimer, Titus S.; Zimmerman, Patrick L.; Stueve, Kirk M.] US Forest Serv, USDA, St Paul, MN 55108 USA. [Seilheimer, Titus S.] Univ Wisconsin, Sea Grant Inst, Manitowoc, WI 54220 USA. [Zimmerman, Patrick L.] Univ Minnesota, Sch Stat, Minneapolis, MN 55455 USA. [Stueve, Kirk M.] Univ Minnesota, Nat Resource Res Inst, Duluth, MN 55811 USA. EM charleshperry@fs.fed.us NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 2-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165105447 ER PT J AU Qui, X Burri, BJ AF Qui, Xiao Burri, Betty J. TI Impact of concentration and variety of oil on the absorption of carotenoids from mandarin oranges and red grapefruit SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Qui, Xiao; Burri, Betty J.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA. EM betty.burri@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 231-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100301 ER PT J AU Raithore, S Manthey, J Plotto, A Bai, JH Baldwin, E AF Raithore, Smita Manthey, John Plotto, Anne Bai, Jinhe Baldwin, Elizabeth TI Identification of compounds responsible for off flavor in Huanglongbing (HLB) infected orange juice by liquid chromatography/mass spectrometry SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Raithore, Smita; Manthey, John; Plotto, Anne; Bai, Jinhe; Baldwin, Elizabeth] ARS, USDA, Ft Pierce, FL 34945 USA. EM smita.raithore@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 4 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 196-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100267 ER PT J AU Rasmussen, M AF Rasmussen, Mark TI USDA Foreign Agricultural Service: Challenges in supporting US growers SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Rasmussen, Mark] USDA, Washington, DC 20250 USA. EM Mark.Rasmussen@fas.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 583-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101049 ER PT J AU Reid, CX Brown, A Sparks, D Mylorie, E Williams, PW AF Reid, Cedric X. Brown, Ashli Sparks, Darrell Mylorie, Erik Williams, Paul W. TI Correlating aflatoxin accumulation and fungal biomass in Aspergillus flavus inoculated maize SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Reid, Cedric X.; Brown, Ashli; Sparks, Darrell] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Starkville, MS 39762 USA. [Mylorie, Erik; Williams, Paul W.] USDA, Res Unit, Corn Host Plant Resistance Res Unit, Starkville, MS 39762 USA. EM cxr1@msstate.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 302-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100667 ER PT J AU Rice, CP Bialek-Kalinski, K McCarty, G AF Rice, Clifford P. Bialek-Kalinski, Krystyna McCarty, Gregory TI Using chiral identification of enantiomers of metolachlor ethane sulfonic acid as a groundwater dating tool SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Rice, Clifford P.; Bialek-Kalinski, Krystyna; McCarty, Gregory] ARS, USDA, Beltsville, MD 20105 USA. EM clifford.rice@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 643-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101106 ER PT J AU Rice, P Fairbairn, D Kaufenberg, E Arnold, W Novak, P Koskinen, W Barber, B Karpuzcu, MEE Swackhamer, D AF Rice, Pamela Fairbairn, David Kaufenberg, Elizabeth Arnold, William Novak, Paige Koskinen, William Barber, Brian Karpuzcu, M. Ekrem Ekrem Swackhamer, Deborah TI Occurrence of pesticides and contaminants of emerging concern in surface waters: Influence of surrounding land use and evaluation of sampling methods SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Rice, Pamela; Koskinen, William] ARS, USDA, St Paul, MN 55108 USA. [Arnold, William; Novak, Paige] Univ Minnesota, Dept Civil Environm & Geoengn, Minneapolis, MN 55455 USA. [Rice, Pamela; Koskinen, William; Barber, Brian] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Karpuzcu, M. Ekrem Ekrem; Swackhamer, Deborah] Univ Minnesota, Water Resources Ctr, St Paul, MN 55108 USA. [Fairbairn, David; Kaufenberg, Elizabeth] Univ Minnesota, Water Resources Sci Grad Program, St Paul, MN 55108 USA. EM Pamela.Rice@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 5 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 481-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100836 ER PT J AU Rimando, AM AF Rimando, Agnes M. TI Pterostilbene: Health benefits and transgenic production SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Rimando, Agnes M.] USDA, Nat Prod Utilizat Res Unit, University, MS 38677 USA. EM agnes.rimando@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 35-IAC PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167400553 ER PT J AU Romero-Flores, A McConnell, LL Hapeman, C Nguyen, A Ramirez, M Torrents, A AF Romero-Flores, Adrian McConnell, Laura L. Hapeman, Cathleen Anh Nguyen Ramirez, Mark Torrents, Alba TI Improving continuous monitoring of VOC emissions from alternative fertilizers SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Romero-Flores, Adrian; McConnell, Laura L.; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Hapeman, Cathleen; Anh Nguyen] ARS, Environm Management & Byprod Utilizat Lab, USDA, Beltsville, MD USA. [Ramirez, Mark] Distr Columbia Water & Sewer Author, Dept Wastewater Treatment, Washington, DC USA. EM aromerof@umd.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 75-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100448 ER PT J AU Salvador, A Muldoon, MT Lin, A Clotilde, LM Carter, JM AF Salvador, Alexandra Muldoon, Mark T. Lin, Andrew Clotilde, Laurie M. Carter, John M. TI Multiplex microbead immunoassay for detection in food of the 10 most common Shiga toxin producing E. coli pathogen serotypes SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Salvador, Alexandra; Carter, John M.] USDA, Produce Safety & Microbiol Res Unit, Albany, CA 94710 USA. [Muldoon, Mark T.] Romer Labs Technol Inc, Res & Dev, Newark, DE 19713 USA. [Lin, Andrew] US FDA, San Francisco Dist Lab, Alameda, CA 94502 USA. [Clotilde, Laurie M.] MagArray Inc, Sunnyvale, CA 94089 USA. EM alexandra.salvador@ars.usda.gov RI Carter, John Mark/K-2485-2015 OI Carter, John Mark/0000-0001-8251-4168 NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 201-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100272 ER PT J AU Sapozhnikova, Y Lehotay, SJ AF Sapozhnikova, Yelena Lehotay, Steven J. TI Comparison of different methods of extraction for incurred contaminants in fish SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Sapozhnikova, Yelena; Lehotay, Steven J.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. EM steven.lehotay@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 402-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100763 ER PT J AU Savary, BJ Chen, MH Zhang, NN Xu, JF Yu, SG AF Savary, Brett J. Chen, Ming-Hsuan Zhang, Ningning Xu, Jianfeng Yu, Shiguang TI Isolation of functional components from red rice bran for evaluating their colon-specific health benefits SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Savary, Brett J.; Zhang, Ningning; Xu, Jianfeng; Yu, Shiguang] Arkansas State Univ, Arkansas Biosci Inst, Jonesboro, AR 72467 USA. [Chen, Ming-Hsuan] ARS, Dale Bumpers Natl Rice Res Ctr, USDA, Stuttgart, AR 72160 USA. EM bsavary@astate.edu NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 234-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100304 ER PT J AU Schmidt, WF Hapeman, CJ Rice, CP McConnell, LL AF Schmidt, Walter F. Hapeman, Cathleen J. Rice, Clifford P. McConnell, Laura L. TI Discerning the flexibility and rigidity of beta-endosulfan isomerization to alpha-endosulfan using temperature-dependent Raman (TDR) spectroscopy: Influences on environmental fate SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Schmidt, Walter F.; Hapeman, Cathleen J.; Rice, Clifford P.] USDA ARS, Beltsville, MD 20705 USA. [McConnell, Laura L.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. EM walter.schmidt@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 479-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100834 ER PT J AU Schmitz, M Greis, C Philipp, O Silva, CJ Schulz-Schaeffer, WJ Wrede, A Koppe, K Onisko, B Requena, JR Govindarajan, N Korth, C Fischer, A Zerr, I AF Schmitz, Matthias Greis, Catharina Philipp, Ottis Silva, Christopher J. Schulz-Schaeffer, Walter J. Wrede, Arne Koppe, Katharina Onisko, Bruce Requena, Jesus R. Govindarajan, Nambirajan Korth, Carsten Fischer, Andre Zerr, Inga TI Physiological role of the normal cellular prion protein (PrPC) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Schmitz, Matthias] Univ Gottingen, Dept Neurol, D-37075 Gottingen, Germany. [Schmitz, Matthias; Greis, Catharina; Zerr, Inga] Univ Med Ctr Gottingen, Dept Neurol, D-37077 Gottingen, Germany. [Philipp, Ottis; Koppe, Katharina; Korth, Carsten] Univ Dusseldorf, Dept Neuropathol, D-40225 Dusseldorf, Germany. [Silva, Christopher J.] ARS, USDA, Albany, CA 94710 USA. [Schulz-Schaeffer, Walter J.; Wrede, Arne] Univ Gottingen, Univ Med Ctr, Dept Neurol, Pr & Dementia Res Unit, D-37075 Gottingen, Germany. [Onisko, Bruce] OpiPro Biosci, Dept Mass Spectrometry, Kensington, CA 94707 USA. [Requena, Jesus R.] Univ Santiago de Compostela, CIMUS Biomed Res Inst, Santiago De Compostela 15782, Galiza, Spain. [Govindarajan, Nambirajan] German Ctr Neurodegenerat Dis DZNE, Dept Med, D-01307 Dresden, Germany. [Govindarajan, Nambirajan; Fischer, Andre; Zerr, Inga] German Ctr Neurodegenerat Dis DZNE, Dept Med, D-37077 Gottingen, Germany. [Fischer, Andre] Univ Med Ctr Gottingen, Dept Psychiat, D-37077 Gottingen, Germany. EM christopher.silva@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 113-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165102024 ER PT J AU Shelver, WL DeSutter, TM AF Shelver, Weilin L. DeSutter, Thomas M. TI Ractopamine uptake from soil by alfalfa (Medicago sativa) and wheat (Triticum aestivum) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Shelver, Weilin L.] USDA ARS, Biosci Res Lab, Fargo, ND 58102 USA. [DeSutter, Thomas M.] N Dakota State Univ, Dept Soil Sci, Fargo, ND 58108 USA. EM Weilin.Shelver@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 463-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100822 ER PT J AU Shen, QY AF Shen, Qiuyun TI Rational design and directed evolution of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus for lactulose synthesis SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Shen, Qiuyun] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China. [Shen, Qiuyun] USDA, WRRC, Albany, CA 94706 USA. EM Qiuyun.Shen@ARS.USDA.GOV NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 261-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100328 ER PT J AU Silva, CJ Dynin, IC Erickson-Beltran, ML Hui, C Carter, JM AF Silva, Christopher J. Dynin, Irina C. Erickson-Beltran, Melissa L. Hui, Colleen Carter, J. Mark TI Oxidized methionine is not a prion-specific covalent modification SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Silva, Christopher J.; Dynin, Irina C.; Erickson-Beltran, Melissa L.; Hui, Colleen; Carter, J. Mark] ARS, USDA, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov RI Carter, John Mark/K-2485-2015 OI Carter, John Mark/0000-0001-8251-4168 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 230-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165102127 ER PT J AU Silva, CJ Erickson-Beltran, ML Dynin, IC AF Silva, Christopher J. Erickson-Beltran, Melissa L. Dynin, Irina C. TI Small molecules and antibodies: A means of distinguishing between PrPC and PrPSc SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Silva, Christopher J.; Erickson-Beltran, Melissa L.; Dynin, Irina C.] ARS, USDA, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 12-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101827 ER PT J AU Silva, CJ Erickson-Beltran, ML Skinner, CB Dynin, IC Hui, C Patfield, SA He, XH AF Silva, Christopher J. Erickson-Beltran, Melissa L. Skinner, Craig B. Dynin, Irina C. Hui, Colleen Patfield, Stephanie A. He, Xiaohua TI Using mass spectrometry to detect and discriminate among Shiga toxins in the attomole range SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Silva, Christopher J.; Erickson-Beltran, Melissa L.; Skinner, Craig B.; Dynin, Irina C.; Hui, Colleen; Patfield, Stephanie A.; He, Xiaohua] ARS, USDA, Albany, CA 94710 USA. EM christopher.silva@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 355-ANYL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101790 ER PT J AU Stipanovic, RD Crutcher, FK Liu, JG Bell, AA AF Stipanovic, Robert D. Crutcher, Frankie K. Liu, Jinggao Bell, Alois A. TI Can a biological reduce the pathogenicity of the plant pathogen Fusarium oxysporum f. sp. vasinfectum by degrading the phytotoxin fusaric acid? SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Stipanovic, Robert D.; Crutcher, Frankie K.; Liu, Jinggao; Bell, Alois A.] ARS, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77802 USA. EM bob.stipanovic@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 750-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101210 ER PT J AU Tan, CX Schrader, KK Khan, IA Rimando, AM AF Tan, Cheng-Xia Schrader, Kevin K. Khan, Ikhlas A. Rimando, Agnes M. TI Synthesis of flavone analogs and activity against the fish bacterial species Flavobacterium columnare SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Tan, Cheng-Xia] Zhejiang Univ Technol, Coll Chem Engn & Mat Sci, Hangzhou 310014, Zhejiang, Peoples R China. [Schrader, Kevin K.; Rimando, Agnes M.] ARS, USDA, University, MS 38677 USA. [Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. EM agnes.rimando@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 183-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100548 ER PT J AU Templeton, PD Wagschal, K Paavola, CD Lee, CC AF Templeton, Paul D. Wagschal, Kurt Paavola, Chad D. Lee, Charles C. TI Lignocellulose hydrolysis by multienzyme complexes SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Templeton, Paul D.; Wagschal, Kurt; Lee, Charles C.] USDA ARS, Albany, CA 94710 USA. [Paavola, Chad D.] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA. EM paul.templeton@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 68-BIOL PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101875 ER PT J AU Throne, SB Wan, D Orts, W Doi, R Wong, D AF Throne, Sarah Batt Wan, David Orts, William Doi, Roy Wong, Dominic TI Cellulase enzyme complex produced by engineered yeast SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Throne, Sarah Batt; Wan, David; Orts, William; Wong, Dominic] ARS, Bioprod Chem & Engn Res Unit, USDA, WRRC, Albany, CA 94710 USA. [Doi, Roy] Univ Calif Davis, Davis, CA 95616 USA. EM sarah.batt@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 240-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100309 ER PT J AU Tsikolia, M Bernier, UR Agramonte, NM Coy, MR Alden, E Becnel, JJ Clark, GG Linthicum, KJ AF Tsikolia, Maia Bernier, Ulrich R. Agramonte, Natasha M. Coy, Monique R. Alden, Estep Becnel, James J. Clark, Gary G. Linthicum, Kenneth J. TI Structure-activity relationship in 34 trifluoromethylphenyl amides against Aedes aegypti SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Tsikolia, Maia; Bernier, Ulrich R.; Agramonte, Natasha M.; Coy, Monique R.; Alden, Estep; Becnel, James J.; Clark, Gary G.; Linthicum, Kenneth J.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM maia.tsikolia@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 342-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100706 ER PT J AU Tunick, MH Ren, DX Paul, M Bonnaillie, LM Tomasula, PM AF Tunick, Michael H. Ren, Daxi X. Paul, Moushumi Bonnaillie, Laetitia M. Tomasula, Peggy M. TI Effects of pasteurization and homogenization on milk during in vitro digestion SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Tunick, Michael H.; Ren, Daxi X.; Paul, Moushumi; Bonnaillie, Laetitia M.; Tomasula, Peggy M.] ARS, ERRC, USDA, Wyndmoor, PA 19038 USA. [Ren, Daxi X.] Zhejiang Univ, Coll Anim Sci, Hangzhou 310003, Zhejiang, Peoples R China. EM michael.tunick@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 175-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100246 ER PT J AU Uchimiya, M AF Uchimiya, Minori TI Multidentate ligand-assisted sorption of Cd(II) on pyrogenic carbonaceous materials SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Uchimiya, Minori] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 63-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165105503 ER PT J AU Vanotti, M Gonzalez, MCG AF Vanotti, Matias Garcia Gonzalez, Maria Cruz TI Removal and recovery of ammonia from livestock wastewater using hydrophobic gas-permeable membranes SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Vanotti, Matias] ARS, USDA, Florence, SC 29501 USA. [Garcia Gonzalez, Maria Cruz] ITACyL, Valladolid, Spain. EM matias.vanotti@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 370-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165105792 ER PT J AU Yao, Q Hapeman, C McConnell, L Li, H Nguyen, A Downey, P Buser, M Holt, G Preuger, J Eichinger, W Ro, K Torrents, A AF Yao, Qi Hapeman, Cathleen McConnell, Laura Li, Hong Anh Nguyen Downey, Peter Buser, Michael Holt, Gregory Preuger, John Eichinger, William Ro, Kyoung Torrents, Alba TI Utilizing vegetative environmental buffers to mitigate ammonia and particulate matter emissions from poultry houses SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Yao, Qi; McConnell, Laura; Torrents, Alba] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Hapeman, Cathleen; Anh Nguyen; Downey, Peter] ARS, USDA, Beltsville, MD 20705 USA. [Li, Hong] Univ Delaware, Coll Agr & Nat Resources, Newark, DE USA. [Buser, Michael] Oklahoma State Univ, Stillwater, OK 74078 USA. [Preuger, John] ARS, USDA, Ames, IA USA. [Eichinger, William] Univ Iowa, Dept Civil & Environm Engn, Iowa City, IA 52242 USA. [Ro, Kyoung] ARS, USDA, Florence, SC USA. [Holt, Gregory] ARS, USDA, Lubbock, TX USA. EM qiyao17@umd.edu NR 0 TC 0 Z9 0 U1 2 U2 3 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 76-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100449 ER PT J AU Yates, SR AF Yates, Scott R. TI AGRO Innovation Award: Protecting environmental resources by controlling emissions of soil fumigants SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Yates, Scott R.] ARS, US Salin Lab, USDA, Riverside, CA 92507 USA. EM scott.yates@ars.usda.gov NR 0 TC 0 Z9 0 U1 2 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 631-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165101095 ER PT J AU Youngblood, JP Moon, RJ AF Youngblood, Jeffrey P. Moon, Robert J. TI Sustainable composites and nanocomposites from biomass derived sources SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Youngblood, Jeffrey P.] Purdue Univ, Sch Mat Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53726 USA. EM jpyoungb@purdue.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 626-POLY PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8KC UT WOS:000349167405739 ER PT J AU Yu, LL Lu, YJ Gao, BY Chen, P AF Yu, Liangli (Lucy) Lu, Yingjian Gao, Boyan Chen, Pei TI HPLC and flow-injection mass spectrometric (FIMS) fingerprinting combined with PCA in differentiating organic and conventional sweet basil leaves SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Yu, Liangli (Lucy); Gao, Boyan] Shanghai Jiao Tong Univ, Dept Food & Nutraceut Sci, Shanghai 200240, Shanghai, Peoples R China. [Yu, Liangli (Lucy); Lu, Yingjian; Gao, Boyan] Univ Maryland, Dept Nutr & Food Sci, College Pk, MD 20742 USA. [Chen, Pei] ARS, Food Composit Lab, Beltsville Human Nutr Res Ctr, USDA, Beltsville, MD 20705 USA. EM liangli.yu@sjtu.edu.cn RI Gao, Boyan/N-9851-2016 NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 48-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100121 ER PT J AU Zeller, WE Mueller-Harvey, I Drake, C Grabber, J AF Zeller, Wayne E. Mueller-Harvey, Irene Drake, Chris Grabber, John TI Purity assessment of condensed tannin fractions by nuclear magnetic resonance (NMR) spectroscopy SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zeller, Wayne E.; Grabber, John] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Mueller-Harvey, Irene; Drake, Chris] Univ Reading, Sch Agr Policy & Dev, Reading, Berks, England. EM wayne.zeller@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 142-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100214 ER PT J AU Zhang, DP Fang, WP Zhou, L Tan, HW Mischke, S Zee, F Meinhardt, L AF Zhang, Dapeng Fang, Wanping Zhou, Lin Tan, Huawei Mischke, Sue Zee, Francis Meinhardt, Lyndel TI Accurate varietal authentication of cacao (Theobroma cacao L.) and tea (Camellia sinensis [L.] Kuntze) using nanofluidic SNP fingerprinting SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zhang, Dapeng; Mischke, Sue; Meinhardt, Lyndel] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. [Fang, Wanping; Zhou, Lin; Tan, Huawei] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China. [Zee, Francis] ARS, USDA, Hilo, HI 96720 USA. EM Dapeng.Zhang@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 70-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100142 ER PT J AU Zhang, YZ Lee, B Du, WX Grauke, L McHugh, T AF Zhang, Yuzhu Lee, Boram Du, Wen-Xian Grauke, Larry McHugh, Tara TI Purification and characterization pecan (Carya Illinoinensis) vicilin, a putative food allergen SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zhang, Yuzhu; Lee, Boram; Du, Wen-Xian; McHugh, Tara] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Grauke, Larry] ARS, USDA, SPA, Southern Plains Agr Res Ctr, College Stn, TX 77485 USA. EM yuzhu.zhang@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 144-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100216 ER PT J AU Zhang, YZ AF Zhang, Yuzhu TI New structural information on food allergens SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zhang, Yuzhu] ARS, PWA, WRRC, USDA, Albany, CA 94710 USA. EM yuzhu.zhang@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 61-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100133 ER PT J AU Zhou, SF Runge, TM Weimer, PJ Hatfield, RD AF Zhou, Shengfei Runge, Troy M. Weimer, Paul J. Hatfield, Ronald D. TI Producing ethanol from alfalfa stems with an acid ensilage pretreatment SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zhou, Shengfei; Runge, Troy M.] Univ Wisconsin, Dept Biol Syst Engn, Madison, WI 53706 USA. [Weimer, Paul J.; Hatfield, Ronald D.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. EM szhou34@wisc.edu NR 0 TC 0 Z9 0 U1 0 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 304-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100369 ER PT J AU Zhou, ZG Hua, B Cao, XY Yang, J Schmidt-Rohrc, K Olkd, D Deng, BL Li, RY Mao, JD AF Zhou, Zhigao Hua, Bin Cao, Xiaoyan Yang, John Schmidt-Rohrc, Klaus Olkd, Dan Deng, Baolin Li, Renying Mao, Jingdong TI Chemical structures of dissolved organic matter from various sources as characterized by solid-state NMR: Insights into molecular signatures in relation to point and nonpoint sources SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zhou, Zhigao; Cao, Xiaoyan; Mao, Jingdong] Old Dominion Univ, Dept Chem & Biochem, Norfolk, VA 23529 USA. [Hua, Bin; Yang, John] Lincoln Univ Missouri, Dept Agr & Environm Sci, Jefferson City, MO 65102 USA. [Schmidt-Rohrc, Klaus] Iowa State Univ, Dept Chem, Ames, IA 50011 USA. [Olkd, Dan] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Deng, Baolin] Univ Missouri, Dept Civil & Environm Engn, Columbia, MO 65211 USA. [Li, Renying] Nanjing Univ Informat Sci & Technol, Dept Appl Meteorol, Nanjing 210044, Jiangsu, Peoples R China. EM huab@lincolnu.edu RI Cao, Xiaoyan/E-3492-2012 OI Cao, Xiaoyan/0000-0001-7571-6482 NR 0 TC 0 Z9 0 U1 0 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 181-ENVR PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165105611 ER PT J AU Zhu, CH La Frano, MR Cai, YM Burri, BJ AF Zhu, Chenghao La Frano, Michael R. Cai, Yimeng Burri, Betty J. TI Impact of traditional African processing and cooking methods on cyanide concentrations in biofortified cassava (Manihot esculenta) SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Zhu, Chenghao; La Frano, Michael R.; Cai, Yimeng; Burri, Betty J.] ARS, Western Human Nutr Res Ctr, USDA, Davis, CA 95616 USA. [Zhu, Chenghao; Cai, Yimeng] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. EM betty.burri@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 2 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 230-AGFD PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100300 ER PT J AU Ziska, LH AF Ziska, Lewis H. TI Temperature, pests, and pesticides SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Ziska, Lewis H.] ARS, USDA, Beltsville, MD 20705 USA. EM lewis.ziska@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 435-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100794 ER PT J AU Ziska, LH AF Ziska, Lewis H. TI Carbon dioxide, climate change, pest biology, and management: A new paradigm for the 21st century SO ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY LA English DT Meeting Abstract CT 248th National Meeting of the American-Chemical-Society (ACS) CY AUG 10-14, 2014 CL San Francisco, CA SP Amer Chem Soc C1 [Ziska, Lewis H.] ARS, USDA, Beltsville, MD 20705 USA. EM lewis.ziska@ars.usda.gov NR 0 TC 0 Z9 0 U1 1 U2 1 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0065-7727 J9 ABSTR PAP AM CHEM S JI Abstr. Pap. Am. Chem. Soc. PD AUG 10 PY 2014 VL 248 MA 292-AGRO PG 1 WC Chemistry, Multidisciplinary SC Chemistry GA CA8JN UT WOS:000349165100657 ER PT J AU Athanassiou, CG Kavallieratos, NG Throne, JE Nakas, CT AF Athanassiou, Christos G. Kavallieratos, Nickolas G. Throne, James E. Nakas, Christos T. TI Competition among Species of Stored-Product Psocids (Psocoptera) in Stored Grain SO PLOS ONE LA English DT Article ID LIPOSCELIS-BOSTRYCHOPHILA PSOCOPTERA; CALLOSOBRUCHUS-MACULATUS F; SUBTERRANEA L. VERDCOURT; POPULATION-GROWTH; INTRASPECIFIC COMPETITION; LEPINOTUS-RETICULATUS; CONSTANT TEMPERATURES; BAMBARA GROUNDNUT; PAETA PSOCOPTERA; WHEAT AB We evaluated the competition among stored-product psocid species by conducting two series of laboratory experiments. In the first series, three species of Liposcelididae were used: Liposcelis bostrychophila, Liposcelis decolor, and Liposcelis paeta. Five adult females of these species were placed in vials containing wheat, either alone or in all possible combinations of two species. The number of adults in the vials was counted after 35, 70, 105, 140, and 175 days. These tests were performed at 25 and 30 degrees C. At 25 degrees C, there were no differences in numbers of L. bostrychophila when this species was reared either alone or with each of the other two species. At 30 degrees C, L. bostrychophila was the dominant species. The presence of L. bostrychophila had a negative effect on the growth of populations of L. decolor and L. paeta. The presence of L. paeta did not affect growth of populations of L. decolor, although the presence of L. decolor occasionally reduced growth of populations of L. paeta. In the second series of tests, L. bostrychophila adult females were placed in vials of wheat either alone or with adult females of Lepinotus reticulatus, at the ratios of (L. bostrychophila:L. reticulatus) 10:0, 9:1, 7:3, 5:5, 3:7, 1:9, and 0:10. These tests were carried out only at 30 degrees C, and the observation periods were the same as for the first series of tests. Liposcelis bostrychophila was the dominant species in this case as well, regardless of the ratio of the parental females. At the end of the experimental period, L. reticulatus was present only in vials that contained this species alone. Our results showed that L. bostrychophila outcompetes the other stored-product psocid species tested. C1 [Athanassiou, Christos G.] Univ Thessaly, Dept Agr Crop Prod & Rural Dev, Lab Entomol & Agr Zool, Nea Ionia Magnissia, Greece. [Kavallieratos, Nickolas G.] Benaki Phytopathol Inst, Dept Entomol & Agr Zool, Lab Agr Entomol, Kifisia, Attica, Greece. [Athanassiou, Christos G.; Kavallieratos, Nickolas G.; Throne, James E.] USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS USA. [Throne, James E.] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA USA. [Nakas, Christos T.] Univ Thessaly, Dept Agr Crop Prod & Rural Dev, Lab Biometry, Nea Ionia Magnissia, Greece. RP Kavallieratos, NG (reprint author), Benaki Phytopathol Inst, Dept Entomol & Agr Zool, Lab Agr Entomol, Kifisia, Attica, Greece. EM nick_kaval@hotmail.com RI Nakas, Christos/F-3052-2011 OI Nakas, Christos/0000-0003-4155-722X NR 56 TC 5 Z9 5 U1 2 U2 11 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 8 PY 2014 VL 9 IS 8 AR e102867 DI 10.1371/journal.pone.0102867 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AR0CG UT WOS:000343231900007 PM 25105507 ER PT J AU Kim, SA Moore, LV Galuska, D Wright, AP Harris, D Grummer-Strawn, LM Merlo, CL Nihiser, AJ Rhodes, DG AF Kim, Sonia A. Moore, Latetia V. Galuska, Deborah Wright, Ashton P. Harris, Diane Grummer-Strawn, Laurence M. Merlo, Caitlin L. Nihiser, Allison J. Rhodes, Donna G. TI Vital Signs: Fruit and Vegetable Intake Among Children - United States, 2003-2010 SO MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT LA English DT Article ID DIETARY-INTAKE AB Background: Eating more fruits and vegetables adds underconsumed nutrients to diets, reduces the risks for leading causes of illness and death, and helps manage body weight. This report describes trends in the contributions of fruits and vegetables to the diets of children aged 2-18 years. Methods: CDC analyzed 1 day of 24-hour dietary recalls from the National Health and Nutrition Examination Surveys from 2003 to 2010 to estimate trends in children's fruit and vegetable intake in cup-equivalents per 1,000 calories (CEPC) and trends by sex, age, race/ethnicity, family income to poverty ratio, and obesity status. Total fruit includes whole fruit (all fruit excluding juice) and fruit juice (from 100% juice, foods, and other beverages). Total vegetables include those encouraged in the Dietary Guidelines for Americans, 2010 (i.e., dark green, orange, and red vegetables and legumes), white potatoes, and all other vegetables. Results: Total fruit intake among children increased from 0.55 CEPC in 2003-2004 to 0.62 in 2009-2010 because of significant increases in whole fruit intake (0.24 to 0.40 CEPC). Over this period, fruit juice intake significantly decreased (0.31 to 0.22 CEPC). Total vegetable intake did not change (0.54 to 0.53 CEPC). No socio-demographic group met the Healthy People 2020 target of 1.1 CEPC vegetables, and only children aged 2-5 years met the target of 0.9 CEPC fruits. Conclusions: Children's total fruit intake increased because of increases in whole fruit consumption, but total vegetable intake remained unchanged. Implications for Public Health Practice: Increased attention to the policies and food environments in multiple settings, including schools, early care and education, and homes might help continue the progress in fruit intake and improve vegetable intake. C1 [Kim, Sonia A.; Moore, Latetia V.; Galuska, Deborah; Wright, Ashton P.; Harris, Diane; Grummer-Strawn, Laurence M.] CDC, Div Nutr Phys Act & Obes, Natl Ctr Chron Dis Prevent & Hlth Promot, Atlanta, GA 30333 USA. [Merlo, Caitlin L.; Nihiser, Allison J.] CDC, Div Populat Hlth, Natl Ctr Chron Dis Prevent & Hlth Promot, Atlanta, GA 30333 USA. [Rhodes, Donna G.] ARS, Food Surveys Res Grp, USDA, Beltsville, MD USA. RP Kim, SA (reprint author), CDC, Div Nutr Phys Act & Obes, Natl Ctr Chron Dis Prevent & Hlth Promot, Atlanta, GA 30333 USA. EM skim3@cdc.gov NR 24 TC 34 Z9 34 U1 1 U2 23 PU CENTER DISEASE CONTROL & PREVENTION PI ATLANTA PA MAILSTOP E-90, ATLANTA, GA 30333 USA SN 0149-2195 EI 1545-861X J9 MMWR-MORBID MORTAL W JI MMWR-Morb. Mortal. Wkly. Rep. PD AUG 8 PY 2014 VL 63 IS 31 BP 671 EP 676 PG 6 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA AN6EH UT WOS:000340687200003 PM 25102415 ER PT J AU Ji, T Liu, ZG Shen, J Shen, F Liang, Q Wu, LM Chen, GH Corona, M AF Ji, Ting Liu, Zhenguo Shen, Jie Shen, Fang Liang, Qin Wu, Liming Chen, Guohong Corona, Miguel TI Proteomics analysis reveals protein expression differences for hypopharyngeal gland activity in the honeybee, Apis mellifera carnica Pollmann SO BMC GENOMICS LA English DT Article DE Hypopharyngeal gland; Quantitative proteomics; iTRAQ; Secreted protein ID ROYAL JELLY PROTEIN; TANDEM MASS-SPECTROMETRY; QUANTITATIVE PROTEOMICS; PROFILE ANALYSIS; GENE-EXPRESSION; ITRAQ; BEES; IDENTIFICATION; BINDING; APIDAE AB Background: Most of the proteins contained in royal jelly (RJ) are secreted from the hypopharyngeal glands (HG) of young bees. Although generic protein composition of RJ has been investigated, little is known about how age-dependent changes on HG secretion affect RJ composition and their biological consequences. In this study, we identified differentially expressed proteins (DEPs) during HG development by using the isobaric tag for relative and absolute quantification (iTRAQ) labeling technique. This proteomic method increases the potential for new protein discovery by improving the identification of low quantity proteins. Results: A total of 1282 proteins were identified from five age groups of worker bees, 284 of which were differentially expressed. 43 (15.1%) of the DEPs were identified for the first time. Comparison of samples at day 6, 9, 12, and 16 of development relative to day 3 led to the unambiguous identification of 112, 117, 127, and 127 DEPs, respectively. The majority of these DEPs were up-regulated in the older worker groups, indicating a substantial change in the pattern of proteins expressed after 3 days. DEPs were identified among all the age groups, suggesting that changes in protein expression during HG ontogeny are concomitant with different states of worker development. A total of 649 proteins were mapped to canonical signaling pathways found in the Kyoto Encyclopedia of Genes and Genomes (KEGG), which were preferentially associated with metabolism and biosynthesis of secondary metabolites. More than 10 key high-abundance proteins were involved in signaling pathways related to ribosome function and protein processing in the endoplasmic reticulum. The results were validated by qPCR. Conclusion: Our approach demonstrates that HG experienced important changes in protein expression during its ontogenic development, which supports the secretion of proteins involved in diverse functions in adult workers beyond its traditional role in royal jelly production. C1 [Ji, Ting; Liu, Zhenguo; Shen, Jie; Shen, Fang; Chen, Guohong] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China. [Liang, Qin] Fujian Agr & Forestry Univ, Coll Bee Sci, Fuzhou, Peoples R China. [Wu, Liming] Chinese Acad Agr Sci, Inst Apiculture Res, Beijing 100193, Peoples R China. [Corona, Miguel] USDA ARS, Bee Res Lab, Beltsville, MD USA. RP Ji, T (reprint author), Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China. EM tji@yzu.edu.cn; ghchen@yzu.edu.cn FU National Natural Science Foundation of China [31172272]; Ministry of Agriculture of China [CARS-45-SYZ6]; Priority Academic Program Development (PAPD) of the Jiangsu Higher Education Institutions; Graduate Education Innovation Project in Jiangsu Province [CXZZ13-0911] FX We would like to thank Prof. Hengmi Cui in Yangzhou University and Associate prof. Huoqing Zheng in Zhengjiang University for the help in preparation of this manuscript. We also appreciate Associate Pro. Dejun Ji, Ya'ni Zhang and Qi Xu in Yangzhou University for their advising on the revision. This work is supported by the Grant from the National Natural Science Foundation of China (No. 31172272), the earmarked fund for Modern Agro-industry Technology Research System from the Ministry of Agriculture of China (CARS-45-SYZ6), the Priority Academic Program Development (PAPD) of the Jiangsu Higher Education Institutions, and the Graduate Education Innovation Project in Jiangsu Province (CXZZ13-0911). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 4 Z9 5 U1 3 U2 35 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD AUG 8 PY 2014 VL 15 AR 665 DI 10.1186/1471-2164-15-665 PG 14 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AN5QA UT WOS:000340645200002 PM 25103401 ER PT J AU Spatz, SJ Volkening, JD Ross, TA AF Spatz, Stephen J. Volkening, Jeremy D. Ross, Teresa A. TI Molecular characterization of the complete genome of falconid herpesvirus strain S-18 SO VIRUS RESEARCH LA English DT Article DE Falconid herpesvirus1; Genome; Sequence; Inclusion body disease; Falcon ID MAREKS-DISEASE-VIRUS; DUCK ENTERITIS VIRUS; INFECTIOUS LARYNGOTRACHEITIS VIRUS; RESTRICTION-ENDONUCLEASE ANALYSIS; INCLUSION-BODY DISEASE; VARICELLA-ZOSTER-VIRUS; PSEUDORABIES VIRUS; DNA-SEQUENCE; CELL-CULTURE; COLUMBID HERPESVIRUS-1 AB Falconid herpesvirus type 1 (FaHV-1) is the causative agent of falcon inclusion body disease, an acute, highly contagious disease of raptors. The complete nucleotide sequence of the genome of FaHV-1 has been determined using Illumina MiSeq sequencing. The genome is 204,054 nucleotides in length and has a class E organization. The genome encodes approximately 130 putative protein-coding genes, of which 70 are orthologs of conserved alphaherpesvirus and Mardivirus proteins. Three FaHV-1 genes (U(L)3.5, U(L)44.5 and CIRC) were identified that encode protein homologues unique to Mardivirus and Varicellovirus. The genome also encodes homologues to the Mardivirus genes LORF2, LORF3, LORF4, LORF5, SORF3 and SORF4. An opal mutation resulting in premature termination was identified in the FaHV-1 U(L)43 gene. Phylogenetically, FaHV-1 resides in a monophyletic group with the other Mardiviruses but, along with anatid herpesvirus 1, represents a more distant divergence from the rest of the Mardivirus genus. Published by Elsevier B.V. C1 [Spatz, Stephen J.; Ross, Teresa A.] USDA ARS, SE Poultry Res Lab, Athens, GA 30605 USA. [Volkening, Jeremy D.] BASE2BIO, Oshkosh, WI 54904 USA. RP Spatz, SJ (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Stephen.Spatz@ars.usda.gov; jeremy@base2bio.com OI Volkening, Jeremy/0000-0002-8892-7155 FU US Department of Agriculture CRIS program [6112-32000-065] FX This research was supported by the US Department of Agriculture CRIS program (project # 6112-32000-065). NR 71 TC 2 Z9 2 U1 0 U2 6 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1702 EI 1872-7492 J9 VIRUS RES JI Virus Res. PD AUG 8 PY 2014 VL 188 BP 109 EP 121 DI 10.1016/j.virusres.2014.03.005 PG 13 WC Virology SC Virology GA AL4TS UT WOS:000339128300015 PM 24685675 ER PT J AU Friedman, M AF Friedman, Mendel TI Chemistry and Multibeneficial Bioactivities of Carvacrol (4-Isopropyl-2-methylphenol), a Component of Essential Oils Produced by Aromatic Plants and Spices SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Review DE carvacrol; biosynthesis; chemistry; analysis; antioxidative effects; anti-inflammatory effects; antimicrobial activities; antiviral effects; antifungal properties; antibiotic resistance; health-promoting potential; mechanisms; food and feed safety; food animals; animal waste odors; human health; research needs ID ESCHERICHIA-COLI O157-H7; ENTERICA SEROVAR TYPHIMURIUM; OREGANO ESSENTIAL OIL; INACTIVATE ANTIBIOTIC-RESISTANT; GLYCOALKALOID ALPHA-CHACONINE; CAMPYLOBACTER-JEJUNI STRAINS; NATURAL PHENOLIC-COMPOUNDS; PATHOGEN BACILLUS-CEREUS; EMBRYO CELL-MEMBRANES; HIBISCUS EDIBLE FILMS AB Aromatic plants produce organic compounds that may be involved in the defense of plants against phytopathogenic insects, bacteria, fungi, and viruses. One of these compounds, called carvacrol, which is found in high concentrations in essential oils such as oregano, has been reported to exhibit numerous bioactivities in cells and animals. This integrated overview surveys and interprets our present knowledge of the chemistry and analysis of carvacrol and its beneficial bioactivities. These activities include its antioxidative properties in food (e.g., lard, sunflower oil) and in vivo and the inhibition of foodbome and human antibiotic-susceptible and antibiotic-resistant pathogenic bacteria, viruses, pathogenic fungi and parasites, and insects in vitro and in human foods (e.g., apple juice, eggs, leafy greens, meat and poultry products, milk, oysters) and food animal feeds and wastes. Also covered are inhibitions of microbial and fungal toxin production and the anti-inflammatory, analgesic, antiarthritic, antiallergic, anticarcinogenic, antidiabetic, cardioprotective, gastroprotective, hepatoprotective, and neuroprotective properties of carvacrol as well as metabolic, synergistic, and mechanistic aspects. Areas for future research are also suggested. The collated information and suggested research might contribute to a better understanding of agronomical, biosynthetic, chemical, physiological, and cellular mechanisms of the described health-promoting effects of carvacrol, and facilitate and guide further studies needed to optimize the use of carvacrol as a multifunctional food in pure and encapsulated forms, in edible antimicrobial films, and in combination with plant-derived and medical antibiotics to help prevent or treat animal and human diseases. C1 ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Friedman, M (reprint author), ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. OI Friedman, Mendel/0000-0003-2582-7517 NR 256 TC 14 Z9 15 U1 7 U2 102 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 6 PY 2014 VL 62 IS 31 BP 7652 EP 7670 DI 10.1021/jf5023862 PG 19 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AM7WT UT WOS:000340080100002 PM 25058878 ER PT J AU Kim, SP Nam, SH Friedman, M AF Kim, Sung Phil Nam, Seok Hyun Friedman, Mendel TI Rice Hull Smoke Extract Protects Mice against a Salmonella Lipopolysaccharide-Induced Endotoxemia SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE rice hull liquid smoke; mice; endotoxemia prevention; mechanism; food preservative; Salmonella lipopolysaccharide; cytokines; myeloperoxidase; oxidative enzymes; histopathology; liver/kidney injuries prevention ID ACUTE LUNG INJURY; LIQUID SMOKE; DIABETIC MICE; SEPTIC SHOCK; MORTALITY; SEPSIS; MODEL; LIVER; INFLAMMATION; MECHANISMS AB Endotoxemia (sepsis, septic shock) is an inflammatory, virulent disease that results mainly from infection by Gram-negative bacteria. The present study investigates the inhibitory effects of a rice hull smoke extract (RHSE) against murine endotoxemia induced by Salmonella lipopolysaccharide and D-galactosamine (LPS/GalN). Pretreatment of the mice with RHSE via dietary administration for 2 weeks resulted in the suppression (in %) of LPS/GalN-induced catalase by 70.7, superoxide dismutase (SOD) by 54.6, and transaminase (GOT/GPT) liver enzymes by 40.6/62.5, the amelioration of necrotic liver lesions, and the reduction of tumor necrosis factor-alpha (TNF-alpha) by 61.1 and nitrite serum level by 83.4, as well as myeloperoxidase (MPO) enzyme associated with necrotic injury of the lung and kidney by 65.7 and 63.3, respectively. The RHSE also extended the lifespan of the toxemic mice. The results using inflammation biomarkers and from the lifespan studies suggest that the RHSE can protect mice against LPS/GalN-induced liver, lung, and kidney injuries and inflammation by blocking oxidative stress and TNF-a production, thereby increasing the survival of the toxic-shock-induced mice. These beneficial effects and previous studies on the antimicrobial effects against Salmonella Typhimurium in culture and in mice suggest that the smoke extract also has the potential to serve as a new multifunctional resource in human food and animal feeds. Possible mechanisms of the beneficial effects at the cellular and molecular levels and suggested food uses are discussed. C1 [Kim, Sung Phil; Nam, Seok Hyun] Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Nam, SH (reprint author), Ajou Univ, Dept Biol Sci, Suwon 443749, South Korea. EM shnam@ajou.ac.kr; Mendel.Friedman@ars.usda.gov OI Friedman, Mendel/0000-0003-2582-7517 NR 44 TC 6 Z9 6 U1 0 U2 7 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 6 PY 2014 VL 62 IS 31 BP 7753 EP 7759 DI 10.1021/jf501533s PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AM7WT UT WOS:000340080100014 PM 25068861 ER PT J AU Williams, JR Rayburn, JR Cline, GR Sauterer, R Friedman, M AF Williams, John Russell Rayburn, James R. Cline, George R. Sauterer, Roger Friedman, Mendel TI Potential Protective Effect of L-Cysteine against the Toxicity of Acrylamide and Furan in Exposed Xenopus laevis Embryos: An Interaction Study SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE acrylamide; furan; Xenopus laevis; embryo; FETAX; L-cysteine; L-cysteine adducts; teratogenicity; interactions; additive response; antagonism; synergism; isobole diagram; mechanisms; binary mixtures; ternary combinations; toxic units; dietary significance; food safety ID TERATOGENESIS ASSAY-XENOPUS; GLYCOALKALOID ALPHA-CHACONINE; DNA ADDUCT FORMATION; DEVELOPMENTAL TOXICITY; RISK-ASSESSMENT; REACTIVE METABOLITE; PROCESSED FOODS; CELL-MEMBRANES; DOSE-RESPONSE; ASCORBIC-ACID AB The embryo toxicities of two food-processing-induced toxic compounds, acrylamide and furan, with and without added L-cysteine were examined individually and in mixtures using the frog embryo teratogenesis assay-Xenopus (FETAX). The following measures of developmental toxicity were used: (a) 96 h LC50, the median concentration causing 50% embryo lethality; (b) 96 h EC50 the median concentration causing 50% malformations of the surviving embryos; and (c) teratogenic index (96 h LC50/96 h EC50), an estimate of teratogenic risk. Calculations of toxic units (TU) were used to assess possible antagonism, synergism, or response addition of several mixtures. The evaluated compounds demonstrated counterintuitive effects. Furan had lower than expected toxicity in Xenopus embryos and, unlike acrylamide, does not seem to be teratogenic. However, the short duration of the tests may not show the full effects of furan if it is truly primarily genotoxic and carcinogenic. L-Cysteine showed unexpected properties in the delay of hatching of the embryos. The results from the interaction studies between combination of two or three components (acrylamide plus L-cysteine; furan plus L-cysteine; acrylamide plus furan; acrylamide plus furan and L-cysteine) show that furan and acrylamide seem to have less than response addition at 1:1 toxic unit ratio in lethality. Acrylamide and L-cysteine show severe antagonism even at low 19 acrylamide/l L-cysteine TU ratios. Data from the mixture of acrylamide, furan, and L-cysteine show a slight antagonism, less than would have been expected from binary mixture exposures. Bioalkylation mechanisms and their prevention are discussed. There is a need to study the toxicological properties of mixtures of acrylamide and furan concurrently formed in heat-processed food. C1 [Williams, John Russell; Rayburn, James R.; Cline, George R.; Sauterer, Roger] Jacksonville State Univ, Dept Biol, Jacksonville, AL 36265 USA. [Friedman, Mendel] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Rayburn, JR (reprint author), Jacksonville State Univ, Dept Biol, Jacksonville, AL 36265 USA. EM jrayburn@jsu.edu OI Friedman, Mendel/0000-0003-2582-7517 FU Jacksonville State University (JSU) Biology Department; JSU FX We thank the Jacksonville State University (JSU) Biology Department and JSU faculty research grants for partial funding for this research. NR 70 TC 3 Z9 3 U1 1 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD AUG 6 PY 2014 VL 62 IS 31 BP 7927 EP 7938 DI 10.1021/jf5013743 PG 12 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AM7WT UT WOS:000340080100035 PM 25055136 ER PT J AU Nigro, D Blanco, A Anderson, OD Gadaleta, A AF Nigro, Domenica Blanco, Antonio Anderson, Olin D. Gadaleta, Agata TI Characterization of Ferredoxin-Dependent Glutamine-Oxoglutarate Amidotransferase (Fd-GOGAT) Genes and Their Relationship with Grain Protein Content QTL in Wheat SO PLOS ONE LA English DT Article ID AMINO-ACID-METABOLISM; BREAD WHEAT; AMMONIUM ASSIMILATION; HEXAPLOID WHEAT; DURUM-WHEAT; SYNTHASE; NITROGEN; BARLEY; PLANTS; PHOTORESPIRATION AB Background: In higher plants, inorganic nitrogen is assimilated via the glutamate synthase cycle or GS-GOGAT pathway. GOGAT enzyme occurs in two distinct forms that use NADH (NADH-GOGAT) or Fd (Fd-GOGAT) as electron carriers. The goal of the present study was to characterize wheat Fd-GOGAT genes and to assess the linkage with grain protein content (GPC), an important quantitative trait controlled by multiple genes. Results: We report the complete genomic sequences of the three homoeologous A, B and D Fd-GOGAT genes from hexaploid wheat (Triticum aestivum) and their localization and characterization. The gene is comprised of 33 exons and 32 introns for all the three homoeologues genes. The three genes show the same exon/intron number and size, with the only exception of a series of indels in intronic regions. The partial sequence of the Fd-GOGAT gene located on A genome was determined in two durum wheat (Triticum turgidum ssp. durum) cvs Ciccio and Svevo, characterized by different grain protein content. Genomic differences allowed the gene mapping in the centromeric region of chromosome 2A. QTL analysis was conducted in the Svevo xCiccio RIL mapping population, previously evaluated in 5 different environments. The study co-localized the Fd-GOGAT-A gene with the marker GWM-339, identifying a significant major QTL for GPC. Conclusions: The wheat Fd-GOGAT genes are highly conserved; both among the three homoeologous hexaploid wheat genes and in comparison with other plants. In durum wheat, an association was shown between the Fd-GOGAT allele of cv Svevo with increasing GPC - potentially useful in breeding programs. C1 [Nigro, Domenica; Blanco, Antonio; Gadaleta, Agata] Univ Bari Aldo Moro, Sect Genet & Plant Breeding, Dept Soil Plant & Food Sci, Bari, Italy. [Anderson, Olin D.] USDA ARS, Genom & Gene Discovery Res Unit, Western Reg Res Ctr, Albany, CA USA. RP Gadaleta, A (reprint author), Univ Bari Aldo Moro, Sect Genet & Plant Breeding, Dept Soil Plant & Food Sci, Bari, Italy. EM agata.gadaleta@uniba.it OI BLANCO, ANTONIO/0000-0002-2222-1133 FU Ministero dell'Istruzione, dell'Universita' e della Ricerca ['PON01_01145-ISCOCEM]; USDA Agricultural Research Service FX The research project was supported by grants from Ministero dell'Istruzione, dell'Universita' e della Ricerca, projects 'PON01_01145-ISCOCEM' and 'PRIN-2010-2011, and by USDA Agricultural Research Service. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 55 TC 2 Z9 3 U1 3 U2 45 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 6 PY 2014 VL 9 IS 8 AR e103869 DI 10.1371/journal.pone.0103869 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM6SM UT WOS:000339995100036 PM 25099972 ER PT J AU Pearson, DE Icasatti, NS Hierro, JL Bird, BJ AF Pearson, Dean E. Icasatti, Nadia S. Hierro, Jose L. Bird, Benjamin J. TI Are Local Filters Blind to Provenance? Ant Seed Predation Suppresses Exotic Plants More than Natives SO PLOS ONE LA English DT Article ID FEEDING-PREFERENCE EXPERIMENTS; HARVESTER ANTS; BIOTIC RESISTANCE; INVASIONS; COMMUNITY; DESERT; DESIGN; SIZE; INVASIVENESS; MYRMECOCHORY AB The question of whether species' origins influence invasion outcomes has been a point of substantial debate in invasion ecology. Theoretically, colonization outcomes can be predicted based on how species' traits interact with community filters, a process presumably blind to species' origins. Yet, exotic plant introductions commonly result in monospecific plant densities not commonly seen in native assemblages, suggesting that exotic species may respond to community filters differently than natives. Here, we tested whether exotic and native species differed in their responses to a local community filter by examining how ant seed predation affected recruitment of eighteen native and exotic plant species in central Argentina. Ant seed predation proved to be an important local filter that strongly suppressed plant recruitment, but ants suppressed exotic recruitment far more than natives (89% of exotic species vs. 22% of natives). Seed size predicted ant impacts on recruitment independent of origins, with ant preference for smaller seeds resulting in smaller seeded plant species being heavily suppressed. The disproportionate effects of provenance arose because exotics had generally smaller seeds than natives. Exotics also exhibited greater emergence and earlier peak emergence than natives in the absence of ants. However, when ants had access to seeds, these potential advantages of exotics were negated due to the filtering bias against exotics. The differences in traits we observed between exotics and natives suggest that higher-order introduction filters or regional processes preselected for certain exotic traits that then interacted with the local seed predation filter. Our results suggest that the interactions between local filters and species traits can predict invasion outcomes, but understanding the role of provenance will require quantifying filtering processes at multiple hierarchical scales and evaluating interactions between filters. C1 [Pearson, Dean E.] Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59808 USA. [Pearson, Dean E.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Hierro, Jose L.] Univ Nacl La Pampa, Consejo Nacl Invest Cient & Tecn, Inst Ciencias Tierra & Ambientales La Pampa, Santa Rosa, La Pampa, Argentina. [Hierro, Jose L.] Univ Nacl La Pampa, Fac Ciencias Exactas & Nat, Santa Rosa, La Pampa, Argentina. [Icasatti, Nadia S.; Bird, Benjamin J.] Forest Serv, Rocky Mt Res Stn, USDA, Ft Collins, CO USA. RP Pearson, DE (reprint author), Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59808 USA. EM dpearson@fs.fed.us FU PECASE (President's Early Career Award in Science and Engineering); Rocky Mountain Research Station, USDA Forest Service FX This project was supported by funding from PECASE (President's Early Career Award in Science and Engineering) and the Rocky Mountain Research Station, USDA Forest Service to DEP. ANPCyT (Agencia Nacional para la Promocion de la Cienciay la Tecnologi a), FCEyN (Facultad de Ciencias Exactas y Naturales), and CIN (Interuniversity National Council of Argentina) Consejo Interuniversitario Nacional to JLH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 54 TC 6 Z9 6 U1 5 U2 39 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 6 PY 2014 VL 9 IS 8 AR e103824 DI 10.1371/journal.pone.0103824 PG 11 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM6SM UT WOS:000339995100033 PM 25099535 ER PT J AU Wilson, WC Bawa, B Drolet, BS Lehiy, C Faburay, B Jasperson, DC Reister, L Gaudreault, NN Carlson, J Ma, W Morozov, I McVey, DS Richt, JA AF Wilson, William C. Bawa, Bhupinder Drolet, Barbara S. Lehiy, Chris Faburay, Bonto Jasperson, Dane C. Reister, Lindsey Gaudreault, Natasha N. Carlson, Jolene Ma, Wenjun Morozov, Igor McVey, D. Scott Richt, Jurgen A. TI Evaluation of lamb and calf responses to Rift Valley fever MP-12 vaccination SO VETERINARY MICROBIOLOGY LA English DT Article DE Rift Valley fever virus; Cattle; Sheep; Vaccine ID GENETIC DIVERSITY; VIRUS-VACCINE; OUTBREAK; SHEEP; PCR; PATHOGENICITY; EPIDEMIC; EFFICACY; ANIMALS; SAFETY AB Rift Valley fever (RVF) is an important viral disease of animals and humans in Africa and the Middle East that is transmitted by mosquitoes. The disease is of concern to international agricultural and public health communities. The RVFV MP-12 strain has been the most safety tested attenuated vaccine strain; thus it is being considered as a potential vaccine for the US national veterinary stockpile. This study was designed to establish safety protocols for large animal research with virulent RVF viruses, establish a target host immune response baseline using RVF MP-12 strain, and independently evaluate this strain as a potential US emergency response vaccine. Ten, approximately four month-old lambs and calves were vaccinated with RVF MP-12 strain; two additional animals per species provided negative control specimens. The animals were monitored for clinical and immune response, fever, and viremia. Two animals per species were sacrificed on 2, 3, 4, 10 and 28 days post infection and full necropsies were performed for histopathological examination. No clinical or febrile responses were observed in this study. The onset and titer of the immune response is discussed. There was no significant histopathology in the lambs; however, 6 out of 10 vaccinated calves had multifocal, random areas of hepatocellular degeneration and necrosis. RVF MP12 antigen was detected in these areas of necrosis by immunohistochemistry in one calf. This study provides independent and baseline information on the RVF MP-12 attenuated vaccination in vaccine relevant age target species and indicates the importance of performing safety testing on vaccine relevant aged target animals. Published by Elsevier B.V. C1 [Wilson, William C.; Drolet, Barbara S.; Lehiy, Chris; Reister, Lindsey; Gaudreault, Natasha N.; McVey, D. Scott] USDA ARS, Ctr Grain & Anim Hlth Res, ABADRU, Manhattan, KS 66502 USA. [Bawa, Bhupinder; Faburay, Bonto; Carlson, Jolene; Ma, Wenjun; Morozov, Igor; Richt, Jurgen A.] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA. [Bawa, Bhupinder; Faburay, Bonto; Carlson, Jolene; Ma, Wenjun; Morozov, Igor; Richt, Jurgen A.] Kansas State Univ, Coll Vet Med, CEEZAD, Manhattan, KS 66506 USA. RP Wilson, WC (reprint author), USDA ARS, Ctr Grain & Anim Hlth Res, ABADRU, Manhattan, KS 66502 USA. EM william.wilson@ars.usda.gov FU Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) [2010-ST061-AG0001]; Kansas Bioscience Authority [58-5430-1-403]; USDA Agricultural Research Service [5430-32000-005-00D] FX The authors thank Drs. Mark Ruder and Myrna Miller for insightful discussions during this study and early review of this manuscript. The authors also thank Mark Minihan and other support staff at Kansas State University Biosecurity Research Institute for their help with animal studies. This work was funded by grants of the Department of Homeland Security Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD), Grant No. 2010-ST061-AG0001, the Kansas Bioscience Authority (agreement number 58-5430-1-403) and the USDA Agricultural Research Service project #5430-32000-005-00D. NR 36 TC 7 Z9 7 U1 0 U2 10 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD AUG 6 PY 2014 VL 172 IS 1-2 BP 44 EP 50 DI 10.1016/j.vetmic.2014.04.007 PG 7 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA AM2QY UT WOS:000339697100006 PM 24856133 ER PT J AU Taus, NS O'Toole, D Herndon, DR Cunha, CW Warg, JV Seal, BS Brooking, A Li, H AF Taus, Naomi S. O'Toole, Donal Herndon, David R. Cunha, Cristina W. Warg, Janet V. Seal, Bruce S. Brooking, Angela Li, Hong TI Malignant catarrhal fever in American bison (Bison bison) experimentally infected with alcelaphine herpesvirus 2 SO VETERINARY MICROBIOLOGY LA English DT Article DE Malignant catarrhal fever; American bison; OvHV-2; A1HV-2 ID VIRAL DIARRHEA VIRUS; ANTIBODY; GENOME AB Malignant catarrhal fever (MCF), due to ovine herpesvirus 2 (OvHV-2), causes appreciable death loss in ranched bison (Bison bison) throughout North America. No vaccine exists to protect animals from disease. Since OvHV-2 has not been propagated in vitro, one strategy to develop a modified live vaccine is to use a closely related, non-pathogenic member of the malignant catarrhal fever virus family as a vector expressing potentially protective OvHV-2 epitopes. To date, no controlled experimental challenge studies with alcelaphine herpesvirus 2 (AIHV-2) derived from topi (Damaliscus lunatus jimela) have been reported The unique or light DNA segment of the AIHV-2 genome was sequenced and annotated and the virus was tested for its ability to infect and induce disease in American bison. Yearling bison were inoculated intranasally (n = 4) or intramuscularly (n = 3) with 2 x 10(-4.7) TCID50 of AIHV-2, and monitored for infection and the development of disease. Six inoculated bison became infected with AIHV-2. Two of the six animals developed clinical signs and had gross and histological lesions consistent with terminal MCF, which differed in distribution from those in bison with MCF due to OvHV-2. One other animal developed minor clinical signs and had gross and histological pulmonary lesions consistent with early (pre-clinical) stages of MCF. Unmodified low cell culture passage AIHV-2 derived from topi is an unsuitable vaccine vector for the prevention of MCF. However, the annotated genome might be useful in identifying genes which could be deleted to potentially attenuate the virus for bison. Published by Elsevier B.V. C1 [Taus, Naomi S.; Herndon, David R.; Li, Hong] Washington State Univ, USDA ARS, ADRU, Pullman, WA 99164 USA. [O'Toole, Donal] Univ Wyoming, Wyoming State Vet Lab, Laramie, WY 82070 USA. [Cunha, Cristina W.; Brooking, Angela] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA. [Warg, Janet V.] USDA, Natl Vet Serv Labs, Ames, IA 50010 USA. [Seal, Bruce S.] USDA ARS, Poultry Microbiol Safety Res Unit, Athens, GA 30605 USA. RP Taus, NS (reprint author), Washington State Univ, USDA ARS, ADRU, Pullman, WA 99164 USA. EM tausns@vetmed.wsu.edu FU USDA-ARS [CWU 5348-32000-032-00D] FX This work was supported by USDA-ARS CWU 5348-32000-032-00D. The authors thank Dr. Chungwon Chung for providing the anti-BVDV antibodies and Shirley Elias, Janice Keller, and Nic Durfee for excellent technical assistance and Rodney Rogers for outstanding animal care and handling. The authors also thank Dr. James Evermann for critical reading of the manuscript. NR 18 TC 1 Z9 1 U1 0 U2 1 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1135 EI 1873-2542 J9 VET MICROBIOL JI Vet. Microbiol. PD AUG 6 PY 2014 VL 172 IS 1-2 BP 318 EP 322 DI 10.1016/j.vetmic.2014.04.003 PG 5 WC Microbiology; Veterinary Sciences SC Microbiology; Veterinary Sciences GA AM2QY UT WOS:000339697100037 PM 24803260 ER PT J AU Theve, M AF Theve, Marissa TI Development of soil halinity classes for Connecticut tidal marshes SO ESTUARINE COASTAL AND SHELF SCIENCE LA English DT Article DE halinity; Connecticut; tidal; marsh; estuary; soil; conductivity ID SALT MARSHES; VEGETATION; SALINITY; RIVER AB A soil's halinity, or ocean derived salinity, can determine much about its use, capabilities, and ecology. This paper uses measurements of electrical conductivity (in a 1 part soil and 5 parts deionized water by volume mixture, EC1:5vol) of estuarine soil samples stratified by ecological community to correlate soil halinity to vegetative community on the Connecticut River. The communities studied here are (in decreasing order of halinity): 1) Low salt marsh/Spartina alterniflora; 2) High salt marsh/Spartina patens, Distichlis spicata, and Juncus gerardii; 3) Back salt marsh/Bolboschoenus and Schoenoplectus species; 4) Brackish marsh/Iva frutescens and Panicum virgatum; and 5) Tidal fresh marsh/Typha and Phragmites species. Out of these, four distinct tidal marsh communities (low salt marsh, high salt marsh, back salt marsh, and tidal fresh) were found to have significantly different mean halinities. These results are a starting place for generating a regional and national soil halinity classification system, and the methods are recommended for use in other estuaries for purposes of ecological classification, soil mapping, and general land/habitat management. Published by Elsevier Ltd. C1 USDA Nat Resources Conservat Serv, Windsor, CT 06084 USA. RP Theve, M (reprint author), USDA Nat Resources Conservat Serv, 344 Merrow Rd,Suite A Tolland, Windsor, CT 06084 USA. EM marissa.theve@gmail.com OI Theve, Marissa/0000-0003-1944-5707 FU United States Department of Agriculture-Natural Resources Conservation Service Soil Science Division FX The author would like to thank the United States Department of Agriculture-Natural Resources Conservation Service Soil Science Division for funding and the Connecticut Natural Resources Conservation Service and University of Connecticut Department of Natural Resources and the Environment staff for technical support. Also, special thanks go to Dr. Juliana Barrett who kindly helped with vegetation and climate change related areas within this project and the anonymous reviewers for their insightful suggestions. NR 40 TC 0 Z9 0 U1 3 U2 10 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0272-7714 EI 1096-0015 J9 ESTUAR COAST SHELF S JI Estuar. Coast. Shelf Sci. PD AUG 5 PY 2014 VL 149 BP 313 EP 319 DI 10.1016/j.ecss.2014.09.011 PG 7 WC Marine & Freshwater Biology; Oceanography SC Marine & Freshwater Biology; Oceanography GA AW3ZF UT WOS:000346220800032 ER PT J AU Hao, GX Pitino, M Ding, F Lin, H Stover, E Duan, YP AF Hao, Guixia Pitino, Marco Ding, Fang Lin, Hong Stover, Ed Duan, Yongping TI Induction of innate immune responses by flagellin from the intracellular bacterium, 'Candidatus Liberibacter solanacearum' SO BMC PLANT BIOLOGY LA English DT Article DE Candidatus Liberibacter solanacearum; Flagellin; Flg22; Cell death; Reactive oxygen species; Gene expression ID PATTERN-RECOGNITION RECEPTORS; PAMP-TRIGGERED IMMUNITY; NICOTIANA-BENTHAMIANA; PLANT DEFENSE; CELL-DEATH; DISEASE RESISTANCE; ARABIDOPSIS; PERCEPTION; SYSTEM; FLS2 AB Background: 'Candidatus Liberibacter solanacearum' (Lso) is a phloem-limited alphaproteobacterium associated with the devastating zebra chip disease of potato (Solanum tuberosum). Like other members of Liberibacter, Lso-ZC1 encodes a flagellin domain-containing protein (Fla(Lso)) with a conserved 22 amino-acid peptide (flg22(Lso)). To understand the innate immune responses triggered by this unculturable intracellular bacterium, we studied the pathogen-associated molecular patterns (PAMPs) that triggered immunity in Nicotiana benthamiana, using the flg22(Lso) peptide and the full length fla(Lso) gene. Results: Our results showed that the expression of fla(Lso) via Agrobacterium-mediated transient expression induced a slow necrotic cell death in the inoculated leaves of N. benthamiana, which was coupled with a burst of reactive oxygen species (ROS) production. Moreover, the expression of several representative genes involved in innate immunity was transiently up-regulated by the flg22(Lso) in N. benthamiana. The Fla(Lso), however, induced stronger up-regulation of these representative genes compared to the flg22(Ls)o, especially that of flagellin receptor FLAGELLIN SENSING2 (FLS2) and respiratory burst oxidase (RbohB) in N. benthamiana. Although neither cell death nor ROS were induced by the synthetic flg22(Lso), a weak callose deposition was observed in infiltrated leaves of tobacco, tomato, and potato plants. Conclusion: The flagellin of Lso and its functional domain, flg22(Lso) share characteristics of pathogen-associated molecular patterns, and trigger unique innate immune responses in N. benthamiana. Slow and weak activation of the innate immune response in host plants by the flagellin of Lso may reflect the nature of its intracellular life cycle. Our findings provide new insights into the role of the Lso flagellin in the development of potato zebra chip disease and potential application in breeding for resistance. C1 [Hao, Guixia; Pitino, Marco; Ding, Fang; Stover, Ed; Duan, Yongping] USDA ARS, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Lin, Hong] USDA ARS, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Duan, YP (reprint author), USDA ARS, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM yongping.duan@ars.usda.gov NR 43 TC 9 Z9 9 U1 1 U2 23 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD AUG 5 PY 2014 VL 14 AR 211 DI 10.1186/s12870-014-0211-9 PG 12 WC Plant Sciences SC Plant Sciences GA AM7PT UT WOS:000340060500001 PM 25091183 ER PT J AU Orr, MC Koch, JB Griswold, TL Pitts, JP AF Orr, Michael C. Koch, Jonathan B. Griswold, Terry L. Pitts, James P. TI Taxonomic utility of niche models in validating species concepts: A case study in Anthophora (Heliophila) (Hymenoptera: Apidae) SO ZOOTAXA LA English DT Article DE Apoidea; Anthophorini; bees; taxonomy; biogeography; Nearctic; MaxEnt; niche modelling ID BEES HYMENOPTERA; MEGACHILIDAE; CONSERVATION; ECOREGIONS; FRIESE AB Taxonomy has far-reaching effects throughout biology, and incorrect taxonomy can be detrimental in many ways. Polymorphic species complexes, many of which exist in the bee genus Anthophora Latreille, lend themselves to such difficulties. This study employs environmental niche mapping (ENM) and traditional morphological analyses to investigate the validity of the subjective synonymy of Anthophora (Heliophila) curta Provancher with the senior synonym A. squammulosa Dours. Eleven of fifty morphological characters consistently differentiate the two putative species, with an additional five characters sometimes separating them. Additionally, based on over 1000 georeferenced museum specimens, the geographic ranges of the two taxa do not overlap. The two entities also react differently to the bioclimatic variables based on correlation analysis. We further tested the two-species hypothesis by constructing ENMs with informative bioclimatic variables associated with locality records. Their modelled distributions overlapped less than 1%, suggesting discrete environmental boundaries. The variables which contributed most to each species' model also differed. These differences are explored in relation to their habitats. The combined morphological and biogeographic analysis indicates that A. curta and A. squammulosa are distinct species. Based on the accumulated evidence the synonymy is formally rejected and A. curta is recognized as a valid species. Five additional taxa (A. bispinosa Cockerell, A. franciscana Cockerell, A. usticauda Cockerell, A. u. cinerior Cockerell, A. zamoranella Cockerell) are newly synonymized with A. squammulosa and Anthophora curta var. melanops Cockerell is newly synonymized with A. curta. Implications outside of taxonomy are discussed. C1 [Orr, Michael C.; Pitts, James P.] Utah State Univ, Dept Biol, Logan, UT 84321 USA. [Koch, Jonathan B.] Utah State Univ, Dept Biol, Ctr Ecol, Logan, UT 84321 USA. [Griswold, Terry L.] ARS, USDA, Pollinating Insects Res Unit, Logan, UT USA. RP Orr, MC (reprint author), Utah State Univ, Dept Biol, Logan, UT 84321 USA. EM michael.christopher.orr@gmail.com; jonathan.koch@usu.edu; terry.griswold@ars.usda.gov; james.pitts@usu.edu OI Orr, Michael/0000-0002-9096-3008 FU National Science Foundation [DBI-0956388]; Community Foundation's Desert Legacy Fund FX We would first like to thank the following individuals for comments on the manuscript: Jim Cane, Frank Parker, Claus Rasmussen, Eugene Schupp, and an anonymous reviewer. We would also like to thank Harold Ikerd for his assistance with the USDA-ARS National Pollinating Insects Collection Database. The mapping involved in this work was made possible by the efforts of numerous curators who made material from their institutions available for study: John Ascher and Eli Wyman, American Museum of Natural History; Jim Berrian, San Diego Natural History Museum; Sean Brady and Brian Harris, United States National Museum; Brian Brown and Weiping Xei; Los Angeles County Museum; Hilary Erenler, University of Northampton; Steve Gaimari and Jackie Kishmirian, California State Collection of Arthropods; Sangmi Lee, Arizona State University; Christopher Marshall, Oregon State University; Frank Merickel, University of Idaho; Bob Minckley, University of Rochester; Peter Oboyski, University of California at Berkeley; Carl Olson, University of Arizona; Agni le Touret-Alby, National Museum of Natural History in France; Cory Sheffield, Royal Saskatchewan Museum; Karen Wright, University of New Mexico; and Doug Yanega, University of California at Riverside. This work was made possible in part by National Science Foundation grant DBI-0956388. Fieldwork of the primary author was supported in part by The Community Foundation's Desert Legacy Fund. NR 36 TC 1 Z9 1 U1 1 U2 16 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD AUG 4 PY 2014 VL 3846 IS 3 BP 411 EP 429 PG 19 WC Zoology SC Zoology GA AM2GO UT WOS:000339668200005 PM 25112259 ER PT J AU Vereecken, H Huisman, JA Pachepsky, Y Montzka, C van der Kruk, J Bogena, H Weihermuller, L Herbst, M Martinez, G Vanderborght, J AF Vereecken, H. Huisman, J. A. Pachepsky, Y. Montzka, C. van der Kruk, J. Bogena, H. Weihermueller, L. Herbst, M. Martinez, G. Vanderborght, J. TI On the spatio-temporal dynamics of soil moisture at the field scale SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil moisture; Spatial variability; Temporal variability; Modeling; Optimal design ID GROUND-PENETRATING RADAR; TIME-DOMAIN REFLECTOMETRY; ELECTRICAL-RESISTIVITY TOMOGRAPHY; SYNTHETIC-APERTURE RADAR; WATER-CONTENT MEASUREMENT; WAVE-FORM INVERSION; ELECTROMAGNETIC INDUCTION MEASUREMENTS; MICROWAVE DIELECTRIC BEHAVIOR; MULTIPLE-POINT GEOSTATISTICS; SGP97 HYDROLOGY EXPERIMENT AB In this paper, we review the state of the art of characterizing and analyzing spatio-temporal dynamics of soil moisture content at the field scale. We discuss measurement techniques that have become available in recent years and that provide unique opportunities to characterize field scale soil moisture variability with high spatial and/or temporal resolution. These include soil moisture sensor networks, hydrogeo-physical measurement techniques, novel remote sensing platforms, and cosmic ray probes. Techniques and methods to analyze soil moisture fields are briefly discussed and include temporal stability analysis, wavelet analysis and empirical orthogonal functions. We revisit local and non-local controls on field scale soil moisture dynamics and discuss approaches to model these dynamics at the field scale. Finally, we address the topic of optimal measurement design and provide an outlook and future research perspectives. (C) 2013 Elsevier B.V. All rights reserved. C1 [Vereecken, H.; Huisman, J. A.; Montzka, C.; van der Kruk, J.; Bogena, H.; Weihermueller, L.; Herbst, M.; Vanderborght, J.] Forschungszentrum Julich GmbH, Agrosphere Inst, D-52425 Julich, Germany. [Pachepsky, Y.; Martinez, G.] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Vereecken, H (reprint author), Forschungszentrum Julich GmbH, Agrosphere Inst, D-52425 Julich, Germany. EM h.vereecken@fz-juelich.de RI Huisman, J.A. (Sander)/I-7078-2012; van der Kruk, Jan/A-5640-2008; Martinez Garcia, Gonzalo/E-3069-2013; OI Montzka, Carsten/0000-0003-0812-8570; van der Kruk, Jan/0000-0003-2348-1436; Herbst, Michael/0000-0003-1371-4208; Huisman, Johan Alexander/0000-0002-1327-0945; Martinez Garcia, Gonzalo/0000-0001-5575-9397; Vanderborght, Jan/0000-0001-7381-3211; Pachepsky, Yakov/0000-0003-0232-6090; Bogena, Heye/0000-0001-9974-6686 NR 296 TC 60 Z9 62 U1 31 U2 167 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG 4 PY 2014 VL 516 SI SI BP 76 EP 96 DI 10.1016/j.jhydrol.2013.11.061 PG 21 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AL3NT UT WOS:000339036100008 ER PT J AU Garcia, GM Pachepsky, YA Vereecken, H AF Martinez Garcia, Gonzalo Pachepsky, Yakov A. Vereecken, Harry TI Effect of soil hydraulic properties on the relationship between the spatial mean and variability of soil moisture SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil moisture; Variability; Soil hydraulic properties; Climate ID TEMPORAL STABILITY; WATER CONTENT; CONDUCTIVITY; SURFACE; FLOW; PERSPECTIVE; MEDIA AB Knowledge of spatial mean soil moisture and its variability over time is needed in many environmental applications. We analyzed dependencies of soil moisture variability on average soil moisture contents in soils with and without root water uptake using ensembles of non-stationary water flow simulations by varying soil hydraulic properties under different climatic conditions. We focused on the dry end of the soil moisture range and found that the magnitude of soil moisture variability was controlled by the interplay of soil hydraulic properties and climate. The average moisture at which the maximum variability occurred depended on soil hydraulic properties and vegetation. A positive linear relationship was observed between mean soil moisture and its standard deviation and was controlled by the parameter defining the shape of soil water retention curves and the spatial variability of saturated hydraulic conductivity. The influence of other controls, such as variable weather patterns, topography or lateral flow processes needs to be studied further to see if such relationship persists and could be used for the inference of soil hydraulic properties from the spatiotemporal variation in soil moisture. Published by Elsevier B.V. C1 [Martinez Garcia, Gonzalo] Univ Cordoba, Dept Agron, E-14071 Cordoba, Spain. [Martinez Garcia, Gonzalo; Pachepsky, Yakov A.] ARS, USDA, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Vereecken, Harry] Forschungszentrum Julich GmbH, Inst Bio & Geosci, Agrosphere IBG 3, D-52428 Julich, Germany. RP Garcia, GM (reprint author), ARS, USDA, Environm Microbial & Food Safety Lab, 10300 Baltimore Ave,BARC East Bldg 173, Beltsville, MD 20705 USA. EM Gonzalo.Martinez@ars.usda.gov RI Martinez Garcia, Gonzalo/E-3069-2013; OI Martinez Garcia, Gonzalo/0000-0001-5575-9397; Pachepsky, Yakov/0000-0003-0232-6090 FU US Department of Agriculture; US Nuclear Regulatory Commission Interagency Agreement [IAA-NRC-05-005]; Spanish Ministry of Education for the mobility [EX2009-0433] FX This study was partially supported by US Department of Agriculture and US Nuclear Regulatory Commission Interagency Agreement IAA-NRC-05-005 on "Model Abstraction Techniques to Simulate Transport in Soils". The first author wishes to thank the Spanish Ministry of Education for the mobility Grant EX2009-0433. The authors would like to thank the valuable and useful contribution of two anonymous referees. NR 33 TC 6 Z9 7 U1 3 U2 40 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG 4 PY 2014 VL 516 SI SI BP 154 EP 160 DI 10.1016/j.jhydrol.2014.01.069 PG 7 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AL3NT UT WOS:000339036100014 ER PT J AU de Jeu, RAM Holmes, TRH Parinussa, RM Owe, M AF de Jeu, Richard A. M. Holmes, Thomas R. H. Parinussa, Robert M. Owe, Manfred TI A spatially coherent global soil moisture product with improved temporal resolution SO JOURNAL OF HYDROLOGY LA English DT Article DE Soil moisture; Dielectric constant; Passive microwave radiometry; Remote sensing ID VEGETATION OPTICAL DEPTH; PASSIVE MICROWAVE OBSERVATIONS; POLARIZATION DIFFERENCE INDEX; AMSR-E; SATELLITE-OBSERVATIONS; RETRIEVAL; MODEL; VALIDATION; CLIMATE; FIELDS AB Global soil moisture products that are completely independent of any type of ancillary data and solely rely on satellite observations are presented. Additionally, we further develop an existing downscaling technique that enhances the spatial resolution of such products to approximately 11 km. These products are based on internal modules of the Land Parameter Retrieval Model (LPRM), an algorithm that uses the radiative transfer equation to link soil moisture, vegetation optical depth and land surface temperature to observed brightness temperatures. The soil moisture product that is independent of any type of ancillary data uses the internally calculated dielectric constant as a soil moisture proxy. This data product is not influenced by errors associated with coarse-scale global soil property maps or by any other type of forcing (e.g. re-analysis) data and is therefore solely based on satellite microwave observations. The second step builds upon recent developments to increase the spatial resolution of the LPRM retrievals using a smoothing filter downscaling method. With this method we can attain a spatial resolution that can be more useful at the scale of local and regional hydrological studies as well. The steps presented in this paper were applied to observations from the Advanced Microwave Scanning Radiometer-Earth Observing System (AMSR-E). The newly derived data sets were validated using ground-based observations from the International Soil Moisture Network (ISMN). The internally calculated dielectric constant product results in significantly more days with valid retrievals than the original soil moisture data products, in particular over arid regions. The dielectric constant product resulted in similar correlations with in situ data as the original soil moisture data product. Together, these findings demonstrate the usefulness of this new dielectric constant product for the hydrological modeling community and climate studies. A case study on the Australian Fitzroy catchment demonstrated that the downscaled data product has a more detailed spatial description of soil moisture, especially during wet and dry conditions with more pronounced dry and wet regions within the catchment. The increased resolution data products could therefore improve runoff predictions and this study demonstrated the potential added value of a transitioning from a spatial resolution of 56 km toward a higher resolution of 11 km. The hydrological implications of these newly developed data records are not only linked to AMSR-E satellite data, but also to the next generation Soil Moisture Active and Passive (SMAP) mission where a 9 km spatial resolution is the target resolution for satellite soil moisture products. The new data products will not replace the current LPRM products, but will be added to the existing array of data products and will become publicly available through our data portals. (C) 2014 Elsevier B.V. All rights reserved. C1 [de Jeu, Richard A. M.; Parinussa, Robert M.] Vrije Univ Amsterdam, Dept Earth Sci Earth & Climate Cluster, NL-1081 HV Amsterdam, Netherlands. [Holmes, Thomas R. H.] ARS, USDA, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Owe, Manfred] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20770 USA. RP de Jeu, RAM (reprint author), Vrije Univ Amsterdam, Dept Earth Sci Earth & Climate Cluster, De Boelelaan 1085, NL-1081 HV Amsterdam, Netherlands. EM r.a.m.de.jeu@vu.nl RI Holmes, Thomas/F-4512-2010 OI Holmes, Thomas/0000-0002-4651-0079 FU European Space Agency Climate Change Initiative for soil moisture [4000104814/11/I-NB]; European Commission [282672] FX This work has been undertaken as part of the European Space Agency Climate Change Initiative for soil moisture (http://www.esa-soilmoisture-cci.org/), Contract No. 4000104814/11/I-NB) Additionally, this work was funded by the European Commission's 7th Framework Project, under Grant Agreement Number 282672, EMBRACE Project. The authors would like to thank the International Soil Moisture Network for making the verification data available. Finally, the authors would like to thank Dr. I. van Meerveld for her valuable contribution to this manuscript. NR 70 TC 20 Z9 20 U1 2 U2 35 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0022-1694 EI 1879-2707 J9 J HYDROL JI J. Hydrol. PD AUG 4 PY 2014 VL 516 SI SI BP 284 EP 296 DI 10.1016/j.jhydrol.2014.02.015 PG 13 WC Engineering, Civil; Geosciences, Multidisciplinary; Water Resources SC Engineering; Geology; Water Resources GA AL3NT UT WOS:000339036100026 ER PT J AU Rao, RSP Thelen, JJ Miernyk, JA AF Rao, R. Shyama Prasad Thelen, Jay J. Miernyk, Jan A. TI In silico analysis of protein Lys-N-epsilon-acetylation in plants SO FRONTIERS IN PLANT SCIENCE LA English DT Review DE acetyltransferase; bromodomain; deacetylase; interactome; post-translational modification; regulation; signaling AB Among post-translational modifications, there are some conceptual similarities between Lys-N-epsilon-acetylation and Ser/Thr/Tyr O-phosphorylation. Herein we present a bioinformatics-based overview of reversible protein Lys-acetylation, including some comparisons with reversible protein phosphorylation. The study of Lys-acetylation of plant proteins has lagged behind studies of mammalian and microbial cells; 1000s of acetylation sites have been identified in mammalian proteins compared with only hundreds of sites in plant proteins. While most previous emphasis was focused on post-translational modifications of histones, more recent studies have addressed metabolic regulation. Being directly coupled with cellular CoA/acetyl-CoA and NAD/NADH, reversible Lys-N-epsilon-acetylation has the potential to control, or contribute to control, of primary metabolism, signaling, and growth and development. C1 [Rao, R. Shyama Prasad; Thelen, Jay J.; Miernyk, Jan A.] Univ Missouri, Div Biochem, 102 Curtis Hall, Columbia, MO 65211 USA. [Rao, R. Shyama Prasad; Thelen, Jay J.; Miernyk, Jan A.] Univ Missouri, Interdisciplinary Plant Grp, Columbia, MO USA. [Miernyk, Jan A.] USDA ARS, Plant Genet Res Unit, Columbia, MO USA. RP Miernyk, JA (reprint author), Univ Missouri, Div Biochem, 102 Curtis Hall, Columbia, MO 65211 USA. EM jan.miernyk@ars.usda.gov RI Rao, R Shyama Prasad/E-5434-2011 OI Rao, R Shyama Prasad/0000-0002-2285-6788 FU National Science Foundation Plant Genome Research Program [DBI-0604439] FX Research in the Thelen Lab is supported by a grant from the National Science Foundation Plant Genome Research Program Grant No. DBI-0604439. NR 122 TC 4 Z9 4 U1 5 U2 8 PU FRONTIERS MEDIA SA PI LAUSANNE PA PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD AUG 4 PY 2014 VL 5 AR 381 DI 10.3389/fpls.2014.00381 PG 11 WC Plant Sciences SC Plant Sciences GA V44SV UT WOS:000209769600001 ER PT J AU Spokas, KA Novak, JM Masiello, CA Johnson, MG Colosky, EC Ippolito, JA Trigo, C AF Spokas, K. A. Novak, J. M. Masiello, C. A. Johnson, M. G. Colosky, E. C. Ippolito, J. A. Trigo, C. TI Physical Disintegration of Biochar: An Overlooked Process SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS LA English DT Article ID DISSOLVED ORGANIC-MATTER; BLACK CARBON; SOIL; CHARCOAL; STABILITY; TRANSPORT; TRANSFORMATION; TEMPERATURE; PARTICLES; ULTISOLS AB Data collected from both artificially and field (naturally) weathered biochar suggest that a potentially significant pathway of biochar disappearance is through physical breakdown of the biochar structure. Via scanning electron microscopy, we characterized this physical weathering that increased the number of structural fractures and yielded higher numbers of liberated biochar fragments. This was hypothesized to be due to the graphitic sheet expansion accompanying water sorption coupled with comminution. These fragments can be on the microscale and the nanoscale but are still carbon-rich particles with no detectable alteration in the oxygen:carbon ratio from that of the original biochar. However, these particles are now easily dissolved and could be moved by infiltration. There is a need to understand how to produce biochars that are resistant to physical degradation to maximize long-term biochar C sequestration potential within soil systems. C1 [Spokas, K. A.] ARS, USDA, St Paul, MN 55018 USA. [Spokas, K. A.; Colosky, E. C.; Trigo, C.] Univ Minnesota, Dept Soil Water & Climate, St Paul, MN 55108 USA. [Novak, J. M.] ARS, USDA, Florence, SC 29501 USA. [Masiello, C. A.] Rice Univ, Dept Earth Sci, Houston, TX 77005 USA. [Masiello, C. A.] Rice Univ, Dept Chem, Houston, TX 77005 USA. [Johnson, M. G.] US EPA, Corvallis, OR 97333 USA. [Ippolito, J. A.] ARS, USDA, Kimberly, ID 83341 USA. RP Spokas, KA (reprint author), USDA ARS, 1991 Upper Buford Circle,439 Borlaug Hall, St Paul, MN 55108 USA. EM kurt.spokas@ars.usda.gov RI Masiello, Caroline/A-2653-2011; Spokas, Kurt/F-4839-2016 OI Masiello, Caroline/0000-0003-2102-6229; Spokas, Kurt/0000-0002-5049-5959 FU U.S. Environmental Protection Agency; Agricultural Research Service of the U.S. Department of Agriculture; National Science Foundation through the Materials Research Science and Engineering Centers program; Minnesota Corn Growers Association/Minnesota Corn Research Production Council; Minnesota Agricultural Utilization Research Institute FX This work has been funded in part by the U.S. Environmental Protection Agency and the Agricultural Research Service of the U.S. Department of Agriculture. It has been subjected to review by the National Health and Environmental Effects Research Laboratory's Western Ecology Division and approved for publication. Approval does not signify that the contents reflect the views of the Agencies, nor does mention of trade names or commercial products constitute endorsement or recommendation for use. We acknowledge the exceptional laboratory work conducted by Martin DuSaire, Eric Nooker, Laura Colosky, Lee Yang, and Rena Weis. Parts of this work were conducted in the Characterization Facility of the University of Minnesota, which receives partial support from National Science Foundation through the Materials Research Science and Engineering Centers program. In addition, we acknowledge the partial funding from the Minnesota Corn Growers Association/Minnesota Corn Research Production Council and the Minnesota Agricultural Utilization Research Institute. This research is part of the USDA-ARS Biochar and Pyrolysis Initiative and USDA-ARS GRACEnet (Greenhouse Gas Reduction through Agricultural Carbon Enhancement Network) programs. We also greatly appreciate the comments from two anonymous reviewers that improved the clarity and impact of this work. NR 45 TC 23 Z9 25 U1 16 U2 66 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2328-8930 J9 ENVIRON SCI TECH LET JI Environ. Sci. Technol. Lett. PD AUG PY 2014 VL 1 IS 8 BP 326 EP 332 DI 10.1021/ez500199t PG 7 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA CD1JO UT WOS:000350831400002 ER PT J AU Johnson, WC Davis, JW AF Johnson, W. Carroll, III Davis, Jerry W. TI Effect of Sprayer Output Volume and Adjuvants on Efficacy of Clove Oil for Weed Control in Organic Vidalia (R) Sweet Onion SO HORTTECHNOLOGY LA English DT Article DE Allium cepa; dry-bulb onion; organic weed control ID HERBICIDE; VINEGAR AB Timely cultivation with a tine weeder and hand weeding are the primary tools for successful weed control in organic sweet onion (Allium cepa), but conditions frequently arise that delay the initial cultivation. Weeds that emerge during the delay are not effectively controlled by cultivation and herbicides derived from natural products may have a role to control the emerged weeds. It has been reported that clove oil herbicide was more effective when sprayers were calibrated for higher output (>50 gal/acre) compared with sprayers calibrated at approximate to 125 gal/acre. However, when clove oil was applied at the recommended rate of 10% by volume, herbicide cost was doubled when sprayer output volume was doubled. It was theorized that herbicide adjuvants might improve clove oil efficacy and reduce weed control cost by not needing to increase sprayer output volume. Trials were conducted from 2010 to 2012 to evaluate all possible combinations of two sprayer output volumes and five herbicide adjuvants used with clove oil (10% by volume) for cool season weed control. Sprayer output volumes evaluated were 25 and 50 gal/acre, using spray tips of differing orifice size. Adjuvants evaluated were a material composed of saponins, citric acid plus garlic extract, an emulsified petroleum oil (EPO) insecticide, a conventional petroleum oil adjuvant (POA), no adjuvant used with clove oil, and a nontreated control. Weed control was not consistently improved by applying clove oil (10% by volume) with a sprayer calibrated at 50 gal/acre compared with sprayer calibrated at 25 gal/acre. Improvements in weed control that were occasionally seen did not affect onion yield. Adjuvants provided minimal improvement in weed control from clove oil and did not consistently improve onion yield. Based on these results, clove oil does not provide suitable levels of weed control in organic Vidalia(R) sweet onion production to justify the expense. C1 [Johnson, W. Carroll, III] USDA ARS, Tifton, GA 31793 USA. [Davis, Jerry W.] Univ Georgia, Griffin, GA 30223 USA. RP Johnson, WC (reprint author), USDA ARS, Tifton Campus,POB 748, Tifton, GA 31793 USA. EM Carroll.Johnson@ars.usda.gov NR 15 TC 1 Z9 1 U1 2 U2 5 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD AUG PY 2014 VL 24 IS 4 BP 428 EP 432 PG 5 WC Horticulture SC Agriculture GA CB6MO UT WOS:000349741300003 ER PT J AU Collier, SM Ruark, MD Oates, LG Jokela, WE Dell, CJ AF Collier, Sarah M. Ruark, Matthew D. Oates, Lawrence G. Jokela, William E. Dell, Curtis J. TI Measurement of Greenhouse Gas Flux from Agricultural Soils Using Static Chambers SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Environmental Sciences; Issue 90; greenhouse gas; trace gas; gas flux; static chamber; soil; field; agriculture; climate ID NITROUS-OXIDE FLUXES; DIFFUSION-MODEL; ATMOSPHERE; EMISSIONS; METHANE AB Measurement of greenhouse gas (GHG) fluxes between the soil and the atmosphere, in both managed and unmanaged ecosystems, is critical to understanding the biogeochemical drivers of climate change and to the development and evaluation of GHG mitigation strategies based on modulation of landscape management practices. The static chamber-based method described here is based on trapping gases emitted from the soil surface within a chamber and collecting samples from the chamber headspace at regular intervals for analysis by gas chromatography. Change in gas concentration over time is used to calculate flux. This method can be utilized to measure landscape-based flux of carbon dioxide, nitrous oxide, and methane, and to estimate differences between treatments or explore system dynamics over seasons or years. Infrastructure requirements are modest, but a comprehensive experimental design is essential. This method is easily deployed in the field, conforms to established guidelines, and produces data suitable to large-scale GHG emissions studies. C1 [Collier, Sarah M.] Univ Wisconsin, Off Sustainabil, Madison, WI 53706 USA. [Ruark, Matthew D.] Univ Wisconsin, Dept Soil Sci, Madison, WI 53706 USA. [Oates, Lawrence G.] Univ Wisconsin, Dept Agron, Madison, WI 53706 USA. [Oates, Lawrence G.] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI 53706 USA. [Jokela, William E.] USDA ARS, Dairy Forage Res Ctr, Washington, DC 20250 USA. [Dell, Curtis J.] USDA ARS, Pasture Syst Watershed Management Res Unit, Washington, DC 20250 USA. RP Dell, CJ (reprint author), USDA ARS, Pasture Syst Watershed Management Res Unit, Washington, DC 20250 USA. EM curtis.dell@ars.usda.gov OI Collier, Sarah/0000-0002-8834-5075; Oates, Lawrence/0000-0003-4829-7600 FU National Science Foundation [1215858]; US Department of Agriculture [2013-68002-20525]; US Department of Energy Great Lakes Bioenergy Research Center - DOE BER Office of Science [DE-FC02-07ER64494]; DOE OBP Office of Energy Efficiency and Renewable Energy [DE-AC05-76RL01830] FX This material is based upon work supported by the National Science Foundation under Grant Number 1215858, by the US Department of Agriculture under Grant Number 2013-68002-20525, and by the US Department of Energy Great Lakes Bioenergy Research Center - DOE BER Office of Science (DE-FC02-07ER64494) and DOE OBP Office of Energy Efficiency and Renewable Energy (DE-AC05-76RL01830). In-field video and images were recorded at the Wisconsin Integrated Cropping System Trial project of the University of Wisconsin-Madison. The authors are grateful to Ryan Curtin for skillful videography and editing. NR 25 TC 2 Z9 2 U1 5 U2 38 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD AUG PY 2014 IS 90 AR e52110 DI 10.3791/52110 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB0EV UT WOS:000349299200080 PM 25146426 ER PT J AU Brisolara, KF Lima, IM Marshall, WE AF Brisolara, Kari Fitzmorris Lima, Isabel M. Marshall, Wayne E. TI Cation and Anion Release from Broiler Litter and Cake Activated Carbons and the Role of Released Anions in Copper Ion Uptake SO WASTE AND BIOMASS VALORIZATION LA English DT Article DE Broiler litter; Broiler cake; Activated carbon; Cations; Anions ID SEWAGE-SLUDGE; POULTRY MANURE; ADSORPTION; METALS; REMOVAL AB Purpose The conversion of broiler waste to activated carbon results in a significant ash fraction that contains several species thought to be responsible for the adsorption capabilities of these carbons. The objectives of this study were to determine (1) the effects of acid washing, a typical regeneration method on the ability of these carbons to adsorb metal cations, (2) the extent to which select cations and anions are released from poultry waste carbons under conditions of varying acid strength, and (3) which of the elements in the ash fraction may contribute to ion binding. Methods Litter and cake activated carbons were placed in solutions of hydrochloric acid at concentrations ranging from 0.05 to 2.0 M for 30 min to 4 h. The resulting solutions were analyzed for the release of the seven select cations and anions. The carbons were then evaluated for their ability to adsorb copper to determine the impact of acid washing on copper adsorption. Results Calcium, phosphorus and magnesium were released in the greatest concentrations (1.7-2.1, 1.6-2.5, and 0.7-0.8 mmol/g respectively). Of these seven species, phosphorus and sulfur were of particular interest due to their potential for binding copper ions. Conclusions The copper adsorption was significantly impacted by the acid washing process with a reduction in adsorption for the litter carbon from 41.96 to 0.32 mg/g and the cake carbon from 85.31 to 23.03 mg/g with an increase in acid concentration from 0.05 to 2.0 M HCl. C1 [Brisolara, Kari Fitzmorris] LSU Hlth Sci Ctr, Sch Publ Hlth, New Orleans, LA 70112 USA. [Lima, Isabel M.; Marshall, Wayne E.] USDA ARS, So Reg Res Ctr, New Orleans, LA 70179 USA. RP Brisolara, KF (reprint author), LSU Hlth Sci Ctr, Sch Publ Hlth, 2020 Gravier St,3rd Floor, New Orleans, LA 70112 USA. EM kbriso@lsuhsc.edu NR 24 TC 1 Z9 1 U1 0 U2 4 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1877-2641 EI 1877-265X J9 WASTE BIOMASS VALORI JI Waste Biomass Valorization PD AUG PY 2014 VL 5 IS 4 BP 689 EP 697 DI 10.1007/s12649-013-9258-3 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY7DI UT WOS:000347721100014 ER PT J AU Bajwa, DS Bajwa, SG Holt, G Srinivasan, R Coffelt, T Nakayama, F Gesch, R AF Bajwa, Dilpreet S. Bajwa, Sreekala G. Holt, Greg Srinivasan, Radhakrishnan Coffelt, Terry Nakayama, Francis Gesch, Russ TI Recycling of Ligno-Cellulosic and Polyethylene Wastes from Agricultural Operations in Thermoplastic Composites SO WASTE AND BIOMASS VALORIZATION LA English DT Article DE Ligno-cellulosic fibers; Thermoplastic composites; Recycled film AB In the US, wood plastic composites represent one of the successful markets for natural fiber-filled thermoplastic composites. In the past several years, the availability of good quality wood fiber has been diminishing and prices of wood and plastic have been increasing. Therefore, the vast quantities of ligno-cellulosic and thermoplastic wastes generated by the agriculture sector could potentially become inexpensive raw materials for thermoplastic composites. This study focuses on the use of selected byproducts from cotton, guayule, cuphea and corn processing operations in the development of natural fiber thermoplastic composites (NFTC). The results indicate that various fibers affect both physical and mechanical properties of composites differently, and could be selectively used to impart desirable properties in composite materials. Overall non-woody ligno-cellulosic fibers showed higher affinity to moisture than woody ligno-cellulosic fibers. C1 [Bajwa, Dilpreet S.] N Dakota State Univ, Dept Mech Engn, Fargo, ND 58108 USA. [Bajwa, Sreekala G.] N Dakota State Univ, Fargo, ND 58108 USA. [Holt, Greg] USDA ARS, Cotton Prod & Proc Res Unit, Lubbock, TX 79403 USA. [Srinivasan, Radhakrishnan] Mississippi State Univ, Mississippi State, MS 39762 USA. [Coffelt, Terry; Nakayama, Francis] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85238 USA. [Gesch, Russ] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. RP Bajwa, DS (reprint author), N Dakota State Univ, Dept Mech Engn, Fargo, ND 58108 USA. EM dilpreet.bajwa@ndsu.edu FU Cotton Inc. FX Funding for this research was provided by Cotton Inc. NR 7 TC 1 Z9 1 U1 1 U2 6 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1877-2641 EI 1877-265X J9 WASTE BIOMASS VALORI JI Waste Biomass Valorization PD AUG PY 2014 VL 5 IS 4 BP 709 EP 714 DI 10.1007/s12649-013-9263-6 PG 6 WC Environmental Sciences SC Environmental Sciences & Ecology GA AY7DI UT WOS:000347721100016 ER PT J AU Infante, F Perez, J Vega, FE AF Infante, F. Perez, J. Vega, F. E. TI The coffee berry borer: the centenary of a biological invasion in Brazil SO BRAZILIAN JOURNAL OF BIOLOGY LA English DT Editorial Material ID HYPOTHENEMUS-HAMPEI; COLEOPTERA; SCOLYTIDAE C1 [Infante, F.; Perez, J.] El Colegio Frontera Sur ECOSUR, Tapachula 30700, Chiapas, Mexico. [Vega, F. E.] ARS, Sustainable Perennial Crops Lab, USDA, Beltsville, MD 20705 USA. RP Infante, F (reprint author), El Colegio Frontera Sur ECOSUR, Carretera Antiguo Aeropuerto Km 2-5, Tapachula 30700, Chiapas, Mexico. EM finfante@ecosur.mx OI Infante, Francisco/0000-0002-7419-7606; Vega, Fernando E./0000-0001-8103-5640 NR 9 TC 3 Z9 3 U1 4 U2 14 PU INT INST ECOLOGY PI SAO CARLOS PA RUA BENTO CARLOS, 750 - CENTRO, SAO CARLOS, SP 00000, BRAZIL SN 1519-6984 EI 1678-4375 J9 BRAZ J BIOL JI Braz. J. Biol. PD AUG PY 2014 VL 74 IS 3 SU S BP 125 EP 126 DI 10.1590/1519-6984.15913 PG 2 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AZ4TH UT WOS:000348215400016 PM 25627374 ER PT J AU Blackburn, MB Martin, PAW Kuhar, D Farrar, RR Gundersen-Rindal, DE AF Blackburn, Michael B. Martin, Phyllis A. W. Kuhar, Daniel Farrar, Robert R., Jr. Gundersen-Rindal, Dawn E. TI Crystalliferous Bacillus cereus group bacteria from a Maryland hardwood forest are dominated by psychrotolerant strains SO MICROBIOLOGYOPEN LA English DT Article DE Bacillus cereus; Bacillus thuringiensis; Bacillus weihenstephanensis; multilocus sequence analysis; psychrotolerance ID WEIHENSTEPHANENSIS; SOIL; DIVERSITY AB Crystal-forming bacteria of the Bacillus cereus group were isolated from soil samples collected at different elevations within a mixed hardwood forest in central Maryland, and their phylogenetic relationships determined by multilocus sequence analysis. The vast majority of isolates obtained were associated with two phylogenetic groups known to be psychrotolerant, with very few isolates representing phylogenetic groups more typically associated with Bacillus thuringiensis. Isolates from the psychrotolerant groups were found to grow on solid media at 7 degrees C. Isolates of 11 highly related, novel sequence types (STs) from the psychrotolerant group that includes Bacillus weihenstephanensis were generally found at higher elevations, and were not associated with soils near streams. Isolates of two related STs from the second psychrotolerant group were nearly always found at the bottoms of ravines near streams, in areas abundant in earthworm castings. C1 [Blackburn, Michael B.; Martin, Phyllis A. W.; Kuhar, Daniel; Farrar, Robert R., Jr.; Gundersen-Rindal, Dawn E.] USDA ARS, Invas Insect Biocontrol & Behav Lab, Beltsville, MD 20705 USA. RP Blackburn, MB (reprint author), USDA ARS, Bldg 011A,Rm 214,BARC-West, Beltsville, MD 20705 USA. EM mike.blackburn@ars.usda.gov FU Agricultural Research Service of the USDA FX This research was funded by the Agricultural Research Service of the USDA. NR 25 TC 0 Z9 0 U1 0 U2 5 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-8827 J9 MICROBIOLOGYOPEN JI MicrobiologyOpen PD AUG PY 2014 VL 3 IS 4 BP 578 EP 584 DI 10.1002/mbo3.189 PG 7 WC Microbiology SC Microbiology GA AY8DL UT WOS:000347784600008 PM 24985699 ER PT J AU Ma, JT Sloan, M Fox, CS Hoffmann, U Smith, CE Saltzman, E Rogers, GT Jacques, PF McKeown, NM AF Ma, Jiantao Sloan, Matthew Fox, Caroline S. Hoffmann, Udo Smith, Caren E. Saltzman, Edward Rogers, Gail T. Jacques, Paul F. McKeown, Nicola M. TI Sugar-Sweetened Beverage Consumption Is Associated with Abdominal Fat Partitioning in Healthy Adults SO JOURNAL OF NUTRITION LA English DT Article ID FOOD FREQUENCY QUESTIONNAIRE; SUBCUTANEOUS ADIPOSE-TISSUE; CARDIOVASCULAR-DISEASE; INSULIN SENSITIVITY; COMPUTED-TOMOGRAPHY; LIPOPROTEIN-LIPASE; VISCERAL ADIPOSITY; METABOLIC SYNDROME; DIABETES-MELLITUS; HEART-DISEASE AB Abdominal adiposity, particularly visceral adipose tissue (VAT), is independently linked to the pathogenesis of diabetes and cardiovascular diseases. Emerging evidence suggests that greater intake of sugar-sweetened beverages (SSBs) may be associated with abnormal fat accumulation in VAT. We examined whether habitual SSB consumption and diet soda intakes are differentially associated with deposition of body fat. We conducted a cross-sectional analysis using previously collected data in 2596 middle-aged adults (1306 men and 1290 women) from the Framingham Heart Study Offspring and Third Generation cohorts. VAT and abdominal subcutaneous adipose tissue (SAT) were measured using multidetector computed tomography. Habitual intake of SSBs and diet soda was assessed by a validated food frequency questionnaire. We observed that SSB consumption was positively associated with VAT after adjustment for SAT and other potential confounders (P-trend < 0.001). We observed an inverse association between SSB consumption and SAT (P-trend = 0.04) that persisted after additional adjustment for VAT (P-trend < 0.001). Higher SSB consumption was positively associated with the VAT-to-SAT ratio (P-trend < 0.001). No significant association was found between diet soda consumption and either VAT or the VAT-to-SAT ratio, but diet soda was positively associated with SAT (P-trend < 0.001). Daily consumers of SSBs had a 10% higher absolute VAT volume and a 15% greater VAT-to-SAT ratio compared with nonconsumers, whereas consumption of diet soda was not associated with either volume or distribution of VAT. C1 [Ma, Jiantao; Rogers, Gail T.; Jacques, Paul F.; McKeown, Nicola M.] Tufts Univ, Nutr Epidemiol Program, Boston, MA 02111 USA. [Smith, Caren E.] Tufts Univ, Nutr & Genom Lab, Boston, MA 02111 USA. [Saltzman, Edward] Tufts Univ, Energy Metab Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Sloan, Matthew] Univ Massachusetts, Sch Med, Worcester, MA USA. [Fox, Caroline S.] NHLBI, Framingham Heart Study, Framingham, MA USA. [Fox, Caroline S.] Brigham & Womens Hosp, Dept Med, Div Endocrinol & Metab, Boston, MA 02115 USA. [Hoffmann, Udo] Harvard Univ, Sch Med, Boston, MA USA. [Hoffmann, Udo] Massachusetts Gen Hosp, Dept Radiol, Boston, MA 02114 USA. RP McKeown, NM (reprint author), Tufts Univ, Nutr Epidemiol Program, Boston, MA 02111 USA. EM nicola.mckeown@tufts.edu FU NIH/National Heart, Lung, and Blood Institute Framingham Heart Study [N01-HC-25195]; Boston University School of Medicine; USDA [58-1950-0-014] FX Supported by NIH/National Heart, Lung, and Blood Institute Framingham Heart Study under contract N01-HC-25195, the Boston University School of Medicine, and USDA agreement 58-1950-0-014. NR 41 TC 8 Z9 8 U1 0 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD AUG PY 2014 VL 144 IS 8 BP 1283 EP 1290 DI 10.3945/jn.113.188599 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AX1GU UT WOS:000346696700022 PM 24944282 ER PT J AU Sigua, GC Hunt, PG Stone, KC Cantrell, KB Novak, JM AF Sigua, Gilbert C. Hunt, Patrick G. Stone, Kenneth C. Cantrell, Keri B. Novak, Jeffrey M. TI Contrasting Effects of Sorghum Biochars and Sorghum Residues on Soil Chemical Changes of Coastal Plains Ultisols With Winter Wheat SO SOIL SCIENCE LA English DT Article DE Sorghum residues; sorghum biochars; Coastal Plains; Ultisols; soil chemical properties; winter wheat ID BLACK CARBON; DREDGED MATERIALS; FORAGE PRODUCTIVITY; LAKE PANASOFFKEE; ORGANIC-MATTER; CROP RESIDUES; MINERALIZATION; FERTILITY; PYROLYSIS; CHARCOAL AB Although most soil properties were improved following application of crop residues and/or pyrolyzed crop residues, there is still a need to pursue additional research that will improve our understanding of the impact of soil fertility enhancement because the effect could vary greatly between uncharred sorghum (Sorghum bicolor L.) residues and pyrolyzed sorghum residues (SR). We hypothesized that sorghum biochars (SB) would deliver more significant impact on soil chemical changes than SR. The objective of this study was to evaluate the contrasting effects of SR and SB with or without supplemental inorganic P on soil chemical changes of Coastal Plains Ultisols with winter wheat. The application rates for SR and SB based on 13.5 Mg ha(-1) yield goal for wheat were 13,000 and 5,200 kg.ha(-1), respectively. Inorganic P was added at the rate of 40 kg P.ha(-1). Our results fully support our hypothesis that SB would deliver more positive effect on the changes of soil chemical properties than the SR. Most of the chemical properties of soils were significantly affected by sorghum treatments (SB + SR) and also varied widely between soil types. Overall, application of SB had small, but significant effects on soil pH, soil electrical conductivity, total carbon, and total nitrogen. Quite notably was the significant difference of these soil properties between soil types. Other aspects of soil chemical changes including extractable P, K, Ca, Mg, Al, and Fe were significantly affected by sorghum treatments. Our results have demonstrated widely differing and contrasting effects between SB and SR on changes in soil chemical properties of Coastal Plains Ultisols. C1 [Sigua, Gilbert C.; Hunt, Patrick G.; Stone, Kenneth C.; Cantrell, Keri B.; Novak, Jeffrey M.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. RP Sigua, GC (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, 2611 West Lucas St, Florence, SC 29501 USA. EM gilbert.sigua@ars.usda.gov NR 75 TC 0 Z9 0 U1 3 U2 13 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD AUG PY 2014 VL 179 IS 8 BP 383 EP 392 DI 10.1097/SS.0000000000000078 PG 10 WC Soil Science SC Agriculture GA AU5DJ UT WOS:000345627100003 ER PT J AU Pi, HW Sharratt, B Feng, G Zhang, XX AF Pi, Huawei Sharratt, Brenton Feng, Gary Zhang, Xiaoxiao TI Comparison of Measured and Simulated Friction Velocity and Threshold Friction Velocity Using SWEEP SO SOIL SCIENCE LA English DT Article DE Wind erosion model; threshold friction velocity; friction velocity; wind erosion; stem area index ID EROSION PREDICTION SYSTEM; WIND EROSION; COLUMBIA PLATEAU; AGRICULTURAL FIELDS; SOIL LOSSES; AERODYNAMIC ROUGHNESS; UNITED-STATES; WEPS; PM10; EMISSION AB Adequately simulating the occurrence and magnitude of erosion using the Wind Erosion Prediction System is critical for resource conservation and improving environmental quality in the United States and other regions of the world. Past observations indicate an insensitivity of the Wind Erosion Prediction System erosion submodel (Single-event Wind Erosion Evaluation Program (SWEEP)) to simulate the occurrence of wind erosion from loess soils during intensifying winds in the Pacific Northwest United States. This insensitivity is attributed to either underestimation of friction velocity (u(*)) or overestimation of threshold friction velocity (u(*)t), but no definitive work has been conducted to validate u(*) and u(*t) in the SWEEP. The objective of this study was to compare measured u(*) and u(*t) with those simulated by the SWEEP. The SWEEP begins to simulate erosion when u(*) exceeds u(*t), thus we compared measured and simulated u(*) and u(*t) of agricultural fields during wind erosion events in eastern Washington. No evidence indicated that the SWEEP consistently overestimated or underestimated u(*). The SWEEP was very sensitive to the stem area index, and estimation of u(*) was better when the SWEEP was used at field sites characterized by a lower rather than a higher stem area index. Our results, however, indicate that the SWEEP overestimated u(*t) during all observed erosion events. Therefore, simulating the occurrence of wind erosion of loess soils in the Pacific Northwest and other arid and semiarid regions of the world may be improved through better estimation of u(*t) in the SWEEP. C1 [Pi, Huawei; Feng, Gary; Zhang, Xiaoxiao] Xinjiang Inst Ecol & Geog, State Key Lab Desert & Oasis Ecol, Urumqi, Peoples R China. [Sharratt, Brenton] Washington State Univ, USDA ARS, Pullman, WA 99164 USA. [Feng, Gary] ARS, USDA, University, MS USA. RP Sharratt, B (reprint author), Washington State Univ, USDA ARS, 215 Johnson Hall, Pullman, WA 99164 USA. EM brenton.sharratt@ars.usda.gov FU National Natural Science Foundation of China [41171019]; Chinese Academy of Sciences; USDA-CSREES Columbia Plateau Wind Erosion/Air Quality Project FX This research was supported by the National Natural Science Foundation of China (Grant No. 41171019), One Hundred Talented Researchers Program of Chinese Academy of Sciences, and the USDA-CSREES Columbia Plateau Wind Erosion/Air Quality Project. NR 28 TC 3 Z9 3 U1 2 U2 10 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD AUG PY 2014 VL 179 IS 8 BP 393 EP 402 DI 10.1097/SS.0000000000000082 PG 10 WC Soil Science SC Agriculture GA AU5DJ UT WOS:000345627100004 ER PT J AU Dolanc, CR Safford, HD Thorne, JH Dobrowski, SZ AF Dolanc, Christopher R. Safford, Hugh D. Thorne, James H. Dobrowski, Solomon Z. TI Changing forest structure across the landscape of the Sierra Nevada, CA, USA, since the 1930s SO ECOSPHERE LA English DT Article DE climate change; demography; elevation; fire suppression; historical data; Forest Inventory and Analysis (FIA); forest structure; latitude; Sierra Nevada; tree density; Vegetation Type Mapping (VTM) ID MIXED-CONIFER FOREST; YOSEMITE-NATIONAL-PARK; MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; TREE MORTALITY-RATES; SUB-ALPINE MEADOWS; LAKE TAHOE BASIN; CLIMATE-CHANGE; FIRE HISTORY; CALIFORNIA VEGETATION AB Understanding the dynamics of forest structure aids inference regarding future forests and their distributions around the world. Over the last few decades, several papers have addressed changing forest structure in the Sierra Nevada, CA, USA, but these studies were limited in scope. We carried out a broad comparison of forest density and composition in the 1930s versus the 2000s for the west slope of the central and northern Sierra Nevada, using the two most extensive data sets available. Forests in this region have endured a long, complex history of human disturbance, and are now experiencing climatic shifts. We subdivided the landscape into elevation and latitude zones and compared historical and modern tree densities within each zone. We compared densities in historical plots to burned and unburned modern plots, as well as densities of individual tree species in historical vs. modern plots for their entire elevational distribution. Density of small trees (10.2-30.4 cm dbh) was significantly higher in the modern data set for all elevations and all latitudes, ranging from 20 to 148% higher. However, density of large trees (>= 61.0 cm) was lower in the modern data set for most elevations and latitudes, ranging from 41% to 60% lower in most zones. Density difference of mid-sized trees (30.5-60.9 cm) was mixed, but was generally higher in modern plots. The pattern of more small trees but fewer large trees held for most individual species as well, but with notable exceptions. Our comparison of burned and unburned plots strongly implicates fire suppression as a driver of increased density of small trees in low-to mid-elevation forests. However, modern high-elevation (>2500 m) forests, where fire suppression impacts should be minimal, were also significantly denser than historical plots. Changing climatic conditions may be driving increased densities of small trees in high elevations, as well as decreased densities of large trees across the region. C1 [Dolanc, Christopher R.; Safford, Hugh D.; Thorne, James H.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Dolanc, Christopher R.; Dobrowski, Solomon Z.] Univ Montana, Dept Forest Management, Missoula, MT 59812 USA. [Safford, Hugh D.] US Forest Serv, USDA, Vallejo, CA 94592 USA. RP Dolanc, CR (reprint author), Univ Calif Davis, Dept Environm Sci & Policy, One Shields Ave, Davis, CA 95616 USA. EM crdolanc@ucdavis.edu NR 89 TC 8 Z9 9 U1 7 U2 40 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 2150-8925 J9 ECOSPHERE JI Ecosphere PD AUG PY 2014 VL 5 IS 8 AR 101 DI 10.1890/ES14-00103.1 PG 26 WC Ecology SC Environmental Sciences & Ecology GA AT7DL UT WOS:000345096900008 ER PT J AU Zhang, LX Ruan, Z Tian, L Lai, JH Zheng, P Liang, ZS Alva, AK AF Zhang, Lixin Ruan, Zhi Tian, Li Lai, Jinghuan Zheng, Peng Liang, Zongsuo Alva, Ashok K. TI FOLIAR-APPLIED UREA MODULATES NITRIC OXIDE SYNTHESIS METABOLISM AND GLYCINEBETAINE ACCUMULATION IN DROUGHT-STRESSED MAIZE SO PAKISTAN JOURNAL OF BOTANY LA English DT Article ID NITRATE REDUCTASE; WATER RELATIONS; ABIOTIC STRESS; PLANTS; SYNTHASE; GROWTH; LOCALIZATION; RESPONSES; SEEDLINGS; SALINITY AB Foliar urea has been proved to play a better positive role in enhancing accumulation of nitric oxide (NO) and glycinebetaine (GB) in maize (Zea mays L.) under drought stress (DS). However, it is unclear how foliar urea affects biosynthetic metabolism of NO and its relationship with GB accumulation. This study was on investigating the effect of foliar- applied urea on seedlings of maize cultivar Zhengdan 958 grown in a hydroponic medium under DS or No DS. Contents of NO and GB and nitric oxide synthase (NOS) activity increased and peaked 12 h after the treatment. Nitrate reductase activity (NRA) followed the similar pattern 6h after the treatment. Under DS foliar urea application increased NR and NOS activity and, thereby, increased NO formation. Therefore, enhancement in activities of both NRA and NOS resulted in an increase of NO accumulation. Foliar- applied urea could induce an increased NO burst by enhanced NO synthesis metabolism as a nitrogen signal, possibly resulting in GB accumulation under DS. C1 [Zhang, Lixin; Ruan, Zhi; Tian, Li; Lai, Jinghuan; Zheng, Peng; Liang, Zongsuo] Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China. [Alva, Ashok K.] USDA ARS, Vegetable & Forage Crops Res Unit, Prosser, WA 99350 USA. RP Zhang, LX (reprint author), Northwest A&F Univ, Coll Life Sci, Yangling 712100, Peoples R China. EM zhanglixin@nwsuaf.edu.cn NR 40 TC 1 Z9 1 U1 3 U2 7 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 EI 2070-3368 J9 PAK J BOT JI Pak. J. Bot. PD AUG PY 2014 VL 46 IS 4 BP 1159 EP 1164 PG 6 WC Plant Sciences SC Plant Sciences GA AS9TH UT WOS:000344583800003 ER PT J AU Delph, RJ Clifford, MJ Cobb, NS Ford, PL Brantley, SL AF Delph, Robert J. Clifford, Michael J. Cobb, Neil S. Ford, Paulette L. Brantley, Sandra L. TI PINYON PINE MORTALITY ALTERS COMMUNITIES OF GROUND-DWELLING ARTHROPODS SO WESTERN NORTH AMERICAN NATURALIST LA English DT Article ID COARSE WOODY DEBRIS; HABITAT COMPLEXITY; ENVIRONMENTAL-STRESS; JUNIPER WOODLANDS; UNITED-STATES; DEAD WOOD; DIE-OFF; FOREST; VEGETATION; ASSEMBLAGES AB We documented the effect of drought-induced mortality of pinyon pine (Pinus edulis Engelm.) on communities of ground-dwelling arthropods. Tree mortality alters, microhabitats utilized by ground-dwelling arthropods by increasing solar radiation, dead woody debris, and understory vegetation. Our major objectives were to determine (1) whether there were changes in community composition, species richness, and abundance of ground-dwelling arthropods associated with pinyon mortality and (2) whether specific habitat characteristics and microhabitats accounted for these changes. We predicted shifts in community composition and increases in arthropod diversity and abundance due to the presumed increased complexity of microhabitats from both standing dead and fallen dead trees. We found significant differences in arthropod community composition between high and low pinyon mortality environments, despite no differences in arthropod abundance or richness. Overall, 22% (51 taxa) of the arthropod community were identified as being indicators of either high or low mortality. Our study corroborates other research indicating that arthropods are responsive to even moderate disturbance events leading to changes in the environment. These arthropod responses can be explained in part due to the increase in woody debris and reduced canopy cover created by tree mortality. C1 [Delph, Robert J.] Dept Nat Resources, Select Engn Serv, Dugway, UT 84022 USA. [Delph, Robert J.; Clifford, Michael J.; Cobb, Neil S.] No Arizona Univ, Merriam Powell Ctr Environm Res, Flagstaff, AZ 86011 USA. [Delph, Robert J.; Clifford, Michael J.; Cobb, Neil S.] No Arizona Univ, Colorado Plateau Museum Arthropod Biodivers, Flagstaff, AZ 86011 USA. [Clifford, Michael J.] Lehigh Univ, Earth & Environm Sci Dept, Bethlehem, PA 18015 USA. [Ford, Paulette L.] USDA, Forest Serv, Rocky Mt Res Stn, Albuquerque, NM 87102 USA. [Brantley, Sandra L.] Univ New Mexico, Museum Southwestern Biol, Albuquerque, NM 87131 USA. RP Delph, RJ (reprint author), Dept Nat Resources, Select Engn Serv, Dugway, UT 84022 USA. EM robert.delph@nau.edu FU USDA Forest Service Rocky Mountain Research Station Middle Rio Grande Ecosystem Management Unit; National Science Foundation [DEB-0443526] FX This research was supported by the USDA Forest Service Rocky Mountain Research Station Middle Rio Grande Ecosystem Management Unit and the National Science Foundation under Grant No. DEB-0443526. We thank Gabriel Lung, Jackie Dorland, Edwin Delph, John-Paul Hodnett, and Jacob Higgins for their assistance in fieldwork and data entry. We acknowledge Jessica Delph for her assistance in plant identifications and field work. NR 63 TC 1 Z9 1 U1 2 U2 10 PU BRIGHAM YOUNG UNIV PI PROVO PA 290 LIFE SCIENCE MUSEUM, PROVO, UT 84602 USA SN 1527-0904 EI 1944-8341 J9 WEST N AM NATURALIST JI West. North Am. Naturalist PD AUG PY 2014 VL 74 IS 2 BP 162 EP 184 PG 23 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AS7BW UT WOS:000344413500003 ER PT J AU Godfroid, J DeBolle, X Roop, RM O'Callaghan, D Tsolis, RM Baldwin, C Santos, RL McGiven, J Olsen, S Nymo, IH Larsen, A Al Dahouk, S Letesson, JJ AF Godfroid, J. DeBolle, X. Roop, R. M., II O'Callaghan, D. Tsolis, R. M. Baldwin, C. Santos, R. L. McGiven, J. Olsen, S. Nymo, I. H. Larsen, A. Al Dahouk, S. Letesson, J. J. TI The quest for a true One Health perspective of brucellosis SO REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES LA English DT Article DE Brucella spp.; Brucellosis; Diagnostic; Epidemiology; Host specificity; Immunity; Infection biology; One Health; Resistance; Zoonosis ID ABORTUS STRAIN RB51; POSITIVE SEROLOGICAL REACTIONS; MARINE MAMMAL BRUCELLA; CD8(+) T-CELL; BOVINE BRUCELLOSIS; IMMUNE-RESPONSES; FERAL SWINE; MELITENSIS INFECTION; CYSTOPHORA-CRISTATA; HOST-RANGE AB One Health is an interdisciplinary collaboration that aims at mitigating risks to human health arising from microorganisms present in non-human animal species, which have the potential to be transmitted and cause disease in humans. Different degrees of scientific collaboration and sectoral integration are needed for different types of zoonotic diseases, depending on the health and associated economic gains that can be expected from a One Health approach. Indeed, mitigating zoonotic risks related to emerging diseases with pandemic potential is different from mitigating risks related to endemic zoonotic diseases like brucellosis. Likewise, management of brucellosis at the wildlife-livestock interface in wildlife conservation areas is in essence different from mitigating transmission of a given Brucella species within its preferential host species, which in turn is different from mitigating the spillover of a given Brucella species to non-preferential host species, humans included. Brucellosis economic models often oversimplify and/or wrongly assess transmission between reservoir hosts and spillover hosts. Moreover, they may not properly value non-market outcomes, such as avoidance of human disease, consumer confidence and conservation biology issues. As a result, uncertainty is such that the economic predictions of these models can be questionable. Therefore, understanding the infection biology of Brucella species is a prerequisite. This paper reviews and highlights important features of the infection biology of Brucella species and the changing epidemiology of brucellosis that need to be integrated into a true One Health perspective of brucellosis. C1 [Godfroid, J.; Nymo, I. H.; Larsen, A.] Univ Tromso, Arctic Univ Norway, Dept Arctic & Marine Biol, N-9010 Tromso, Norway. [Godfroid, J.] Univ Pretoria, Fac Vet Sci, Dept Vet Trop Dis, ZA-0110 Onderstepoort, South Africa. [DeBolle, X.; Letesson, J. J.] Univ Namur, Dept Biol, URBM, B-5000 Namur, Belgium. [Roop, R. M., II] E Carolina Univ, Brody Sch Med, Dept Microbiol & Immunol, Greenville, NC 27858 USA. [O'Callaghan, D.] INSERM, U1047, UFR Med, F-30908 Nimes 2, France. [O'Callaghan, D.] Univ Montpellier I, UFR Med, F-30908 Nimes 2, France. [Tsolis, R. M.] Univ Calif Davis, Sch Med, Dept Med Microbiol & Immunol, Davis, CA 95616 USA. [Baldwin, C.] Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA. [Santos, R. L.] Univ Fed Minas Gerais, Escola Vet, Dept Clin & Cirurgia Vet, Belo Horizonte, MG, Brazil. [McGiven, J.] Anim Hlth Vet Labs Agcy, WHO FAO UN Collaborating Ctr Brucellosis, World Org Anim Hlth Reference Lab Brucellosis, Weybridge KT15 2NB, Surrey, England. [Olsen, S.] ARS, Bacterial Dis Livestock Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Al Dahouk, S.] Fed Inst Risk Assessment, D-12277 Berlin, Germany. RP Godfroid, J (reprint author), Univ Tromso, Arctic Univ Norway, Dept Arctic & Marine Biol, N-9010 Tromso, Norway. EM Jacgues.godfroid@uit.no RI Santos, Renato/A-1134-2008; APHA, Staff publications/E-6082-2010; OI Santos, Renato/0000-0002-4830-0470; Al Dahouk, Sascha/0000-0003-3835-0818 NR 114 TC 4 Z9 4 U1 5 U2 27 PU OFFICE INT EPIZOOTIES PI PARIS PA 12 RUE DE PRONY, 75017 PARIS, FRANCE SN 0253-1933 J9 REV SCI TECH OIE JI Rev. Sci. Tech. Off. Int. Epizoot. PD AUG PY 2014 VL 33 IS 2 BP 521 EP 538 PG 18 WC Veterinary Sciences SC Veterinary Sciences GA AR8SP UT WOS:000343845000014 PM 25707181 ER PT J AU Badenes-Perez, FR Castillo, A Johnson, MT AF Badenes-Perez, Francisco R. Castillo, Alexander Johnson, M. Tracy TI Damage to Miconia calvescens and Seasonal Abundance of Salbia lotanalis (Lepidoptera: Crambidae) in Costa Rica SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE leaf roller; defoliation; leaf area ID INVASIVE TREE; DC MELASTOMATACEAE; BIOLOGICAL-CONTROL; ECOLOGY; IMPACT; PLANT AB Miconia calvescens de Candolle (Melastomataceae) is an invasive tree considered the most serious threat to natural ecosystems of Hawaii and other Pacific islands. The success of M. calvescens as an invasive species is greatly owing to its shade tolerance and the shaded habitat it creates, where many native plant species that are light-demanding cannot survive. Salbia lotanalis Druce (Lepidoptera: Crambidae), a neotropical leaf roller attacking M. calvescens, was evaluated for two mechanisms by which it reduces leaf area of its host plant: feeding (defoliation), which removes leaf tissue, and tying leaf rolls, which reduces exposed area of leaves. These impacts were quantified over a 1-yr period at a field site in Costa Rica, where densities of S. lotanalis larvae attacking M. calvescens peaked at the end of the rainy season and declined in the dry season. Up to 47.5% of leaves were attacked by S. lotanalis, with cumulative defoliation by an undetermined number of larvae removing an average of approximate to 30% (253 cm(2)) of each leaf attacked. Defoliation and leaf rolling were compared in a greenhouse experiment in which individual S. lotanalis larvae defoliated an average of 3.7% (17.8 cm(2)) of each attacked leaf, and reduced exposed leaf area as a result of leaf rolling by an average of 12.8% (66.2 cm(2)). Our results complement the findings of previous studies of S. lotanalis and confirm its potential as a biological control agent of M. calvescens. C1 [Badenes-Perez, Francisco R.] Univ Hawaii Manoa, Pacific Cooperat Studies Unit, Honolulu, HI 96822 USA. [Castillo, Alexander] Univ Costa Rica, Escuela Biol, San Jose, Costa Rica. [Johnson, M. Tracy] US Forest Serv, USDA, Pacific Southwest Res Stn, Inst Pacific Isl Forestry, Volcano, HI 96785 USA. RP Badenes-Perez, FR (reprint author), CSIC, Inst Ciencias Agr, E-28006 Madrid, Spain. EM frbadenes@ica.csic.es RI Badenes-Perez, Francisco/K-9722-2014 OI Badenes-Perez, Francisco/0000-0002-8954-7920 FU Hawaii Invasive Species Council; U.S. Department of Agriculture-Forest Service International Programs FX Special thanks to Paul Hanson, Edgar Rojas, and other colleagues at the Universidad de Costa Rica. Funding was provided by the Hawaii Invasive Species Council and U.S. Department of Agriculture-Forest Service International Programs. We also thank the Instituto Meteorologico Nacional de Costa Rica for providing weather data. NR 28 TC 1 Z9 1 U1 1 U2 6 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 877 EP 882 DI 10.1603/EN12345 PG 6 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800004 PM 25182612 ER PT J AU Schmid, RB Lehman, RM Lundgren, JG AF Schmid, Ryan B. Lehman, R. Michael Lundgren, Jonathan G. TI Sex-Specific Interactions of Microbial Symbioses on Cricket Dietary Selection SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Gryllus pennsylvanicus; Chenopodium album; Ephestia kuehniella; nutrition; bacteria ID FEEDING-BEHAVIOR; NUTRITIONAL ECOLOGY; CELLULOSE DIGESTION; HARMONIA-AXYRIDIS; LIFE-HISTORY; COLEOPTERA; BACTERIA; COCCINELLIDAE; SEGREGATION; POPULATIONS AB The nutrients found in prey and nonprey foods, and relative digestibility of these foods, has a major influence on diet selection by omnivorous insects. Many insects have developed symbiotic relationships with gut bacteria to help with extracting nutrition from nonprey diets. Gryllus pennsylvanicus (Burmeister) (Orthoptera: Gryllidae) was assigned to one of two treatment groups, antibiotic-treated and nonantibiotic-treated, and consumption of seeds (nonprey) and eggs (prey) were measured. Male crickets administered antibiotics consumed more seeds and greater seed weight, while antibiotic-fed female crickets consumed fewer seeds and less seed weight, relative to the untreated male and female crickets, respectively. Both male and female antibiotic-treated crickets consumed similar weight of eggs as nonantibiotic-treated male and female crickets, respectively. These results provide evidence that gut symbionts influence diet selection of male and female G. pennsylvanicus differently. This sex-specific dietary selection may be because of the fact that male and female crickets have different nutritional requirements. C1 [Schmid, Ryan B.] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Schmid, Ryan B.; Lehman, R. Michael; Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. RP Lundgren, JG (reprint author), USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. EM Jonathan.Lundgren@ars.usda.gov OI Lehman, Michael/0000-0002-3391-3178 NR 47 TC 2 Z9 2 U1 7 U2 22 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 896 EP 902 DI 10.1603/EN13311 PG 7 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800007 PM 24914929 ER PT J AU Yee, WL Nash, MJ Goughnour, RB Cha, DH Linn, CE Feder, JL AF Yee, Wee L. Nash, Meralee J. Goughnour, Robert B. Cha, Dong H. Linn, Charles E., Jr. Feder, Jeffrey L. TI Ammonium Carbonate Is More Attractive Than Apple and Hawthorn Fruit Volatile Lures to Rhagoletis pomonella (Diptera: Tephritidae) in Washington State SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE apple maggot fly; ammonia; western apple fruit volatile; ornamental hawthorn fruit volatile; western Washington ID WESTERN UNITED-STATES; MAGGOT FLIES DIPTERA; HOST-FRUIT; ODOR DISCRIMINATION; SYMPATRIC SPECIATION; CRATAEGUS SPP.; FIELD-TESTS; TRAPS; FLY; IDENTIFICATION AB The apple maggot fly, Rhagoletis pomonella (Walsh), is an introduced, quarantine pest of apple (Malus domestica Borkhausen) in the Pacific Northwest of the United States. In the eastern United States where the fly is native, fruit volatiles have been reported to be more attractive than ammonia compounds to R. pomonella. However, the opposite may be true in the western United States. Here, we determined whether newly identified western apple and western hawthorn fruit volatiles are more attractive than ammonium carbonate (AC) to R. pomonella in apple, black hawthorn, and ornamental hawthorn trees in western Washington State. In all three host trees, sticky red sphere or yellow panel traps baited with AC generally caught more flies than traps baited with lures containing the four newly developed fruit blends (modified eastern apple, western apple, western ornamental hawthorn, and western black hawthorn) or two older blends (eastern apple and eastern downy hawthorn). Fruit volatiles also displayed more variation among trapping studies conducted at different sites, in different host trees, and across years than AC. The results imply that traps baited with AC represent the best approach to monitoring R. pomonella in Washington State. C1 [Yee, Wee L.; Cha, Dong H.] ARS, USDA, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Nash, Meralee J.] Washington State Univ, Puyallup Res & Extens Ctr, Puyallup, WA 98371 USA. [Goughnour, Robert B.] Washington State Univ Extens, Vancouver, WA 98665 USA. [Linn, Charles E., Jr.] Cornell Univ, Dept Entomol, NYS Agr Expt Stn, Geneva, NY 14456 USA. [Feder, Jeffrey L.] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN USA. RP Yee, WL (reprint author), ARS, USDA, Yakima Agr Res Lab, 5230 Konnowac Pass Rd, Wapato, WA 98951 USA. EM wee.yee@ars.usda.gov FU Washington State Commission on Pesticide Registration; WA Tree Fruit Research Commission; U.S. Department of Agriculture-Foreign Agricultural Service [USDA-FAS] FX We thank John Stark (Washington State University, Puyallup) for providing research space in Puyallup; Doug Stienbarger (Washington State University, Vancouver) and Clark County Heritage Farm for laboratory space in Vancouver; the staff at the U.S. Department of Agriculture-Forest Service for use of the Skamania County site; and Peter Chapman (U.S. Department of Agriculture-Agricultural Research Service [USDA-ARS], Wapato, WA) for laboratory assistance. We also thank Mike Klaus (Washington State Department of Agriculture, Yakima, WA) and Glen Hood (University of Notre Dame) for reviewing earlier drafts of the manuscript, and the two anonymous referees for helpful comments. This research was partially supported by Washington State Commission on Pesticide Registration, WA Tree Fruit Research Commission, and U.S. Department of Agriculture-Foreign Agricultural Service [USDA-FAS] grants to W.L.Y. This article reports results of research only. Mention of a proprietary product does not constitute an endorsement for its use by USDA. NR 43 TC 5 Z9 5 U1 0 U2 12 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 957 EP 968 DI 10.1603/EN14038 PG 12 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800014 PM 24915519 ER PT J AU Zeilinger, AR Olson, DM Andow, DA AF Zeilinger, Adam R. Olson, Dawn M. Andow, David A. TI A Likelihood-Based Biostatistical Model for Analyzing Consumer Movement in Simultaneous Choice Experiments SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE attraction rate; host selection; leaving rate; movement ecology; transient dynamics ID FEEDING-PREFERENCE EXPERIMENTS; STATE-SPACE MODELS; VECTOR PREFERENCE; ANIMAL MOVEMENT; DESIGN; SELECTION; PREY; ECOLOGY; SYSTEM; PESTS AB Consumer feeding preference among resource choices has critical implications for basic ecological and evolutionary processes, and can be highly relevant to applied problems such as ecological risk assessment and invasion biology. Within consumer choice experiments, also known as feeding preference or cafeteria experiments, measures of relative consumption and measures of consumer movement can provide distinct and complementary insights into the strength, causes, and consequences of preference. Despite the distinct value of inferring preference from measures of consumer movement, rigorous and biologically relevant analytical methods are lacking. We describe a simple, likelihood-based, biostatistical model for analyzing the transient dynamics of consumer movement in a paired-choice experiment. With experimental data consisting of repeated discrete measures of consumer location, the model can be used to estimate constant consumer attraction and leaving rates for two food choices, and differences in choice-specific attraction and leaving rates can be tested using model selection. The model enables calculation of transient and equilibrial probabilities of consumer-resource association, which could be incorporated into larger scale movement models. We explore the effect of experimental design on parameter estimation through stochastic simulation and describe methods to check that data meet model assumptions. Using a dataset of modest sample size, we illustrate the use of the model to draw inferences on consumer preference as well as underlying behavioral mechanisms. Finally, we include a user's guide and computer code scripts in R to facilitate use of the model by other researchers. C1 [Zeilinger, Adam R.] Univ Minnesota, Dept Entomol, Conservat Biol Program, St Paul, MN 55108 USA. [Olson, Dawn M.] USDA ARS, Crop Protect & Res Management Unit, Tifton, GA 31793 USA. [Andow, David A.] Univ Minnesota, Dept Entomol, St Paul, MN 55108 USA. [Andow, David A.] Univ Minnesota, Ctr Community Genet, St Paul, MN 55108 USA. RP Zeilinger, AR (reprint author), Univ Calif Berkeley, Berkeley Initiat Global Change Biol, 3101 Valley Life Sci Bldg, Berkeley, CA 94720 USA. EM arz@berkeley.edu OI Zeilinger, Adam/0000-0002-4111-3568 FU National Institute of Food and Agriculture [2008-02409]; IGERT grant from U.S. National Science Foundation; Dayton Wilkie Fund from the Graduate School and Bell Museum of Natural History, University of Minnesota; National Research Initiative of the U.S. Department of Agriculture FX This study was partially supported by a grant from the National Research Initiative of the U.S. Department of Agriculture, National Institute of Food and Agriculture (grant 2008-02409) to D. A. A. and D.M.O., an IGERT grant from U.S. National Science Foundation to the University of Minnesota, and a Thesis Research Grant, Doctoral Dissertation Fellowship, and grants from the Dayton Wilkie Fund from the Graduate School and Bell Museum of Natural History, University of Minnesota, to A.R.Z. We thank the R Help online community for assistance with R programming, and M. Ganesh and R. Almeida for access to the Lawrence Berkeley Laboratory's Computation Genomics Research Laboratory computing cluster to run the stochastic simulation. We also thank C. Neuhauser, M. Daugherty, K. Anderson, H. Regan, J. Sarhad, A. Fahimipour, S. Hayes, H. Hulton, P. Rueda-Cedil, and R. Swab for helpful comments on earlier drafts. NR 48 TC 1 Z9 1 U1 1 U2 9 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 977 EP 988 DI 10.1603/EN13287 PG 12 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800016 PM 25182616 ER PT J AU Gillette, NE Kegley, SJ Costello, SL Mori, SR Webster, JN Mehmel, CJ Wood, DL AF Gillette, Nancy E. Kegley, Sandra J. Costello, Sheryl L. Mori, Sylvia R. Webster, Jeffrey N. Mehmel, Constance J. Wood, David L. TI Efficacy of Verbenone and Green Leaf Volatiles for Protecting Whitebark and Limber Pines From Attack by Mountain Pine Beetle (Coleoptera: Curculionidae: Scolytinae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE green leaf volatile (GLV); pheromone flake; Pinus albicaulis; Pinus flexilis; semiochemical ID NORTHWEST FOREST PLAN; DENDROCTONUS-PONDEROSAE; ANTI-ATTRACTANT; BARK VOLATILES; LODGEPOLE PINE; UNITED-STATES; WESTERN; PHEROMONE; ACETOPHENONE; CONSERVATION AB To develop safe and effective methods to protect whitebark pines, Pinus albicaulis Engelmann, and limber pines, Pinus flexilis James, from attack by mountain pine beetle, Dendroctonus ponderosae Hopkins (Coleoptera: Curculionidae), we compared verbenone and verbenone plus green leaf volatiles (GLVs) for prevention of beetle attack. We used two strategies: area-wide protection where semiochemical-releasing flakes are dispersed over the forest floor, and individual tree tests where flakes are applied to tree trunks. The area-wide bioassays were conducted by applying verbenone- and GLV-releasing flakes without stickers to the forest floor on 0.81-ha plots dominated by whitebark pines in the State of Washington with four replicates. We conducted individual tree bioassays by applying the same formulations with stickers to whitebark and limber pines in Montana and Colorado, respectively. In all three situations, both verbenone-alone and verbenone plus GLVs significantly increased the proportion of trees escaping mass attack by beetles, but the two formulations were not significantly different from one another. Despite a lack of significance at a Bonferroni-adjusted alpha = 0.05, adding GLVs gave slightly greater absolute levels of tree protection in most cases. Monitoring traps placed in the area-wide treatments in Washington showed similar outcomes for numbers of beetles trapped: both treatments had significantly fewer beetles than controls, and they were not significantly different from one another. At peak flight, however, plots with GLVs combined with verbenone had roughly 40% fewer beetles than plots with verbenone alone. GLVs are considerably cheaper than verbenone, so tests of higher application rates may be warranted to achieve enhanced tree protection at reasonable cost. C1 [Gillette, Nancy E.] US Forest Serv, USDA, Pacific SW Res Stn, Albany, CA 94710 USA. [Kegley, Sandra J.] US Forest Serv, USDA, Coeur Dalene, ID 83815 USA. [Costello, Sheryl L.] US Forest Serv, USDA, Golden, CO 80401 USA. [Mori, Sylvia R.] US Forest Serv, USDA, Pacific SW Res Stn, Albany, CA 94710 USA. [Webster, Jeffrey N.] Forestry Consulting, Redding, CA 96002 USA. [Mehmel, Constance J.] US Forest Serv, USDA, Forest Insect & Dis Ctr, Wenatchee, WA 98801 USA. [Wood, David L.] Univ Calif Berkeley, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. RP Gillette, NE (reprint author), US Forest Serv, USDA, Pacific SW Res Stn, 800 Buchanan St, Albany, CA 94710 USA. EM ngillette2@fs.fed.us FU U.S. Department of Agriculture (USDA) Forest Service Special Technology Development Program FX We dedicate this article to the memory of Sheryl Costello, whose untimely death in 2011 was an enormous loss to her family, her friends, and the forest entomology community. She is greatly missed by all. This study was funded by a grant from the U.S. Department of Agriculture (USDA) Forest Service Special Technology Development Program to S.J.K., N.E.G., and C.J.M. We thank Luke Stratford (Yellowstone Club, Big Sky, MT) for assistance with plot location and access to the MT site; Steve Munson (USDA Forest Service, Ogden, UT) for logistical support; Elizabeth Gonzales and Joyce Wong (University of California, Berkeley, CA) for identifying and counting beetles; Roy Magelssen, Darci Carlson (USDA Forest Service, Wenatchee, WA), Doug Wulff (USDA Forest Service, Coeur d'Alene, ID), Adam Messing, and Tom Roland (USDA Forest Service, Ft. Collins, CO) for assistance with field work. Gaylord Briggs, Ed Fredrickson, and Eric Webster (Webster Forestry Consulting, Redding, CA) provided assistance with field work and data entry. Jim Baldwin and Christopher Fettig (USDA Forest Service, Pacific Southwest Research Station, Albany and Davis, CA), Dan Miller (USDA Forest Service, Southern Research Station, Athens, GA), and Brice McPherson (University of California, Berkeley, CA) provided helpful reviews. The use of trade names and identification of firms or corporations does not constitute an official endorsement or approval by the U.S. Government of any product or service to the exclusion of others that may be suitable. The USDA is an equal opportunity provider and employer. NR 45 TC 0 Z9 0 U1 1 U2 20 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 1019 EP 1026 DI 10.1603/EN12330 PG 8 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800021 PM 25003785 ER PT J AU Nehme, ME Trotter, RT Keena, MA McFarland, C Coop, J Hull-Sanders, HM Meng, P De Moraes, CM Mescher, MC Hoover, K AF Nehme, M. E. Trotter, R. T. Keena, M. A. McFarland, C. Coop, J. Hull-Sanders, H. M. Meng, P. De Moraes, C. M. Mescher, M. C. Hoover, K. TI Development and Evaluation of a Trapping System for Anoplophora glabripennis (Coleoptera: Cerambycidae) in the United States SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE Anoplophora glabripennis; monitoring trap; trapping distance; male-produced pheromone; kairomone ID ASIAN LONGHORNED BEETLE; MALE-PRODUCED PHEROMONE; PLANT VOLATILES; ATTRACTION AB Anoplophora glabripennis (Motschulsky) (Coleoptera: Cerambycidae), commonly known as the Asian longhorned beetle, is an invasive wood-boring pest that infests a number of hardwood species and causes considerable economic losses in North America, several countries in Europe, and in its native range in Asia. The success of eradication efforts may depend on early detection of introduced populations; however, detection has been limited to identification of tree damage (oviposition pits and exit holes), and the serendipitous collection of adults, often by members of the public. Here we describe the development, deployment, and evaluation of semiochemical-baited traps in the greater Worcester area in Massachusetts. Over 4 yr of trap evaluation (2009-2012), 1013 intercept panel traps were deployed, 876 of which were baited with three different families of lures. The families included lures exhibiting different rates of release of the male-produced A. glabripennis pheromone, lures with various combinations of plant volatiles, and lures with both the pheromone and plant volatiles combined. Overall, 45 individual beetles were captured in 40 different traps. Beetles were found only in traps with lures. In several cases, trap catches led to the more rapid discovery and management of previously unknown areas of infestation in the Worcester county regulated area. Analysis of the spatial distribution of traps and the known infested trees within the regulated area provides an estimate of the relationship between trap catch and beetle pressure exerted on the traps. Studies continue to optimize lure composition and trap placement. C1 [Nehme, M. E.; Meng, P.; De Moraes, C. M.; Mescher, M. C.; Hoover, K.] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Nehme, M. E.; Meng, P.; De Moraes, C. M.; Mescher, M. C.; Hoover, K.] Penn State Univ, Ctr Chem Ecol, University Pk, PA 16802 USA. [Nehme, M. E.] Lebanon Reforestat Initiat, Mathaf 00961700093, Lebanon. [Trotter, R. T.; Keena, M. A.] US Forest Serv, No Res Stn, Hamden, CT 06514 USA. [McFarland, C.] USDA APHIS, ALB Cooperat Eradicat Program, Worcester, MA 01606 USA. [Coop, J.] Massachusetts Dept Conservat & Recreat, ALB Cooperat Eradicat Program, Worcester, MA 01606 USA. [Hull-Sanders, H. M.] USDA APHIS PPQ, Ctr Plant Hlth Sci & Technol, Buzzards Bay, MA 02542 USA. [De Moraes, C. M.; Mescher, M. C.] Swiss Fed Inst Technol, Dept Environm Syst Sci, CH-8092 Zurich, Switzerland. RP Keena, MA (reprint author), US Forest Serv, No Res Stn, 51 Mill Pond Rd, Hamden, CT 06514 USA. EM mkeena@fs.fed.us FU Alphawood Foundation; Horticultural Research Institute; USDA Forest Service, State and Private Forestry Northeastern Area, Technology & Methods Development [10-CA-11420004-316]; USDA; APHIS; PPQ (Plant Protection and Quarantine) FX We thank B. Aflague, K. Benkoski, G. Bradford, J. Birosack, P. Elder, C. Fagan, J. Franklin, A. Hoover, D. Long, J. McNeil, D. Mikus, W. Panagakos, V. Pavao, C. Perry, D. Roberts, A. Roth, V. Sanchez, E. Schwartzberg, B. Secskas, and M. Shaiyen for technical support in setting up and servicing the traps. We thank K. Freeman, R. Mack, A. Sawyer, C. Markham, D. Lance, V. Mastro, and the survey crew at the ALB Eradication Program Office in Worcester, MA, for assistance with obtaining information on infested trees or help in surveying around positive traps. This work was supported by grants to K.H. from the Alphawood Foundation, the Horticultural Research Institute, and cooperative agreements with the USDA, APHIS, PPQ (Plant Protection and Quarantine), and USDA Forest Service, State and Private Forestry Northeastern Area, Technology & Methods Development 10-CA-11420004-316. NR 28 TC 12 Z9 13 U1 4 U2 42 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 1034 EP 1044 DI 10.1603/EN14049 PG 11 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800023 PM 24960252 ER PT J AU Yocum, GD Rinehart, JP Kemp, WP AF Yocum, George D. Rinehart, Joseph P. Kemp, William P. TI Cell Position During Larval Development Affects Postdiapause Development in Megachile rotundata (Hymenoptera: Megachilidae) SO ENVIRONMENTAL ENTOMOLOGY LA English DT Article DE maternal effect; alfalfa leafcutting bee; Megachile rotundata ID ALFALFA LEAFCUTTING BEE; SOLITARY BEE; SEX-RATIO; INCUBATION TEMPERATURES; EMERGENCE; DIAPAUSE; SURVIVAL; OSMIA; POLLINATION; DISTANCE AB Megachile rotundata (F.) (Hymenoptera: Megachilidae) is the primary pollinator of alfalfa in the northwestern United States and western Canada and provides pollination services for onion, carrot, hybrid canola, various legumes, and other specialty crops. M. rotundata females are gregarious, nest in cavities either naturally occurring or in artificial nesting blocks, where they construct a linear series of brood cells. Because of the physical layout of the nest, the age of the larvae within the nest and the microenvironment the individual larvae experience will vary. These interacting factors along with other maternal inputs affect the resulting phenotypes of the nest mates. To further our understanding of in-nest physiology, gender and developmental rates were examined in relationship to cell position within the nest. Eighty-two percent of the females were located within the first three cells, those furthest from the nest entrance. For those individuals developing in cells located in the deepest half of the nest, the sex of the previous bee had a significant effect on the female decision of the gender of the following nest mate. Removing the prepupae from the nest and rearing them under identical conditions demonstrated that position within the nest during larval development had a significant effect on the postdiapause developmental rates, with males whose larval development occurred deeper in the nest developing more slowly than those toward the entrance. No positional effect on postdiapause developmental rates was noted for the females. The cell position effect on male postdiapause developmental rate demonstrates that postdiapause development is not a rigid physiological mechanism uniform in all individuals, but is a dynamic plastic process shaped by past environmental conditions. C1 [Yocum, George D.; Rinehart, Joseph P.; Kemp, William P.] USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, Fargo, ND 58102 USA. RP Yocum, GD (reprint author), USDA ARS, Red River Valley Agr Res Ctr, Biosci Res Lab, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM george.yocum@ars.usda.gov NR 37 TC 0 Z9 0 U1 2 U2 19 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0046-225X EI 1938-2936 J9 ENVIRON ENTOMOL JI Environ. Entomol. PD AUG PY 2014 VL 43 IS 4 BP 1045 EP 1052 DI 10.1603/EN13222 PG 8 WC Entomology SC Entomology GA AQ9YF UT WOS:000343214800024 PM 24914676 ER PT J AU Atwood, DK Andersen, HE Matthiss, B Holecz, F AF Atwood, Donald K. Andersen, Hans-Erik Matthiss, Benjamin Holecz, Francesco TI Impact of Topographic Correction on Estimation of Aboveground Boreal Biomass Using Multi-temporal, L-Band Backscatter SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Advanced land observing satellite phased array-type L-band synthetic aperture radar (ALOS PALSAR); remote sensing; synthetic aperture radar (SAR) ID SYNTHETIC-APERTURE RADAR; FOREST BIOMASS; SAR DATA; INTERIOR ALASKA; GROUND BIOMASS; TERRAIN INFLUENCES; IMAGERY; REGIONS; INTERFEROMETRY; SCATTERING AB Synthetic aperture radar (SAR) has been shown to be a useful tool for estimating aboveground biomass (AGB), due to the strong correlation between the biomass and backscatter. In particular, L-band SAR is effective for estimating the lower range of biomass that characterizes most boreal forests. Unfortunately, the topographic impact on backscatter can dominate the normal forest signal variation. Since many boreal environments have significant topography, we investigate several topographic correction techniques to determine their effect upon AGB prediction accuracy. Different approaches to addressing the topography include: 1) no correction, 2) local incidence angle (LIA) correction, 3) pixel-area correction, and 4) a novel empirical slope correction. The investigation was performed for a data-rich experimental area near Tok, Alaska, for which Advanced Land Observing Satellite Phased Array type L-Band Synthetic Aperture Radar (ALOS PALSAR), field plots, lidar acquisitions, and a high-quality digital elevation model (DEM) existed. Biomass estimations were performed using both single-and dual-polarization (HH and HV) regressions against field plot data. The biomass estimation for each of the topographic corrections was compared with the field plot biomass, as well as more extensive lidar biomass estimations. The results showed a clear improvement in AGB estimation accuracy from no correction, to LIA, to pixel-area, to the novel pixel-area plus empirical slope correction. Using the field plot data for validation, the SAR root mean square error (RMSE) derived from the best approach was found to be 37.3 Mg/ha over a biomass range of 0-250 Mg/ha, only marginally less accurate than the 33.5 Mg/ha accuracy of the much more expensive lidar technique. C1 [Atwood, Donald K.] Univ Alaska Fairbanks, Inst Geophys, Fairbanks, AK 99775 USA. [Andersen, Hans-Erik] Univ Washington, USDA, US Forest Serv, Pacific NW Res Stn, Seattle, WA 98195 USA. [Matthiss, Benjamin] KIT, IHE, D-76128 Karlsruhe, Germany. [Holecz, Francesco] Sarmap SA, CH-6989 Purasca, Switzerland. RP Atwood, DK (reprint author), Univ Alaska Fairbanks, Inst Geophys, Fairbanks, AK 99775 USA. EM dkatwood@alaska.edu; handersen@fs.fed.us; benjamin.matthiss@student.kit.edu; fholecz@sarmap.ch NR 51 TC 4 Z9 4 U1 0 U2 9 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD AUG PY 2014 VL 7 IS 8 BP 3262 EP 3273 DI 10.1109/JSTARS.2013.2289936 PG 12 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA AQ8DZ UT WOS:000343055200008 ER PT J AU Bright, BC Hudak, AT Kennedy, RE Meddens, AJH AF Bright, Benjamin C. Hudak, Andrew T. Kennedy, Robert E. Meddens, Arjan J. H. TI Landsat Time Series and Lidar as Predictors of Live and Dead Basal Area Across Five Bark Beetle-Affected Forests SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Forestry; image sequence analysis; remote sensing; vegetation mapping ID MOUNTAIN PINE-BEETLE; WESTERN UNITED-STATES; DISCRETE-RETURN LIDAR; TM TASSELED CAP; TREE MORTALITY; MULTISPECTRAL DATA; SATELLITE DATA; CLIMATE-CHANGE; TIMBER VOLUME; BIOMASS AB Bark beetle-caused tree mortality affects important forest ecosystem processes. Remote sensing methodologies that quantify live and dead basal area (BA) in bark beetle-affected forests can provide valuable information to forest managers and researchers. We compared the utility of light detection and ranging (lidar) and the Landsat-based detection of trends in disturbance and recovery (LandTrendr) algorithm to predict total, live, dead, and percent dead BA in five bark beetle-affected forests in Alaska, Arizona, Colorado, Idaho, and Oregon, USA. The BA response variables were predicted from lidar and LandTrendr predictor variables using the random forest (RF) algorithm. RF models explained 28%-61% of the variation in BA responses. Lidar variables were better predictors of total and live BA, whereas LandTrendr variables were better predictors of dead and percent dead BA. RF models predicting percent dead BA were applied to lidar and LandTrendr grids to produce maps, which were then compared to a gridded dataset of tree mortality area derived from aerial detection survey (ADS) data. Spearman correlations of beetle-caused tree mortality metrics between lidar, LandTrendr, and ADS were low to moderate; low correlations may be due to plot sampling characteristics, RF model error, ADS data subjectivity, and confusion caused by the detection of other types of forest disturbance by LandTrendr. Provided these sources of error are not too large, our results show that lidar and LandTrendr can be used to predict and map live and dead BA in bark beetle-affected forests with moderate levels of accuracy. C1 [Bright, Benjamin C.; Hudak, Andrew T.] US Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Kennedy, Robert E.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Meddens, Arjan J. H.] Univ Idaho, Dept Geog, Moscow, ID 83844 USA. RP Bright, BC (reprint author), US Forest Serv, Rocky Mt Res Stn, Moscow, ID 83843 USA. EM benjamincbright@fs.fed.us; ahudak@fs.fed.us; kennedyr@bu.edu; ameddens@uidaho.edu FU Special Technology Development Program of the U.S. Forest Service Forest Health Protection division; Oregon State University [09.JV.11261952.014]; University of Idaho [11-JV-11221633-184]; National Park Service and the Los Alamos National Laboratory FX This work was supported in part by the Special Technology Development Program of the U.S. Forest Service Forest Health Protection division, in part by Oregon State University for LandTrendr processing provided through Joint Venture Agreement 09.JV.11261952.014, and in part by the University of Idaho for lidar processing through Joint Venture Agreement 11-JV-11221633-184. The work of A. J. H. Meddens was supported by cooperative agreements with the National Park Service and the Los Alamos National Laboratory. NR 73 TC 3 Z9 4 U1 1 U2 32 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD AUG PY 2014 VL 7 IS 8 BP 3440 EP 3452 DI 10.1109/JSTARS.2014.2346955 PG 13 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA AQ8DZ UT WOS:000343055200023 ER PT J AU Qiu, JX Crow, WT Mo, XG Liu, SX AF Qiu, Jianxiu Crow, Wade T. Mo, Xingguo Liu, Suxia TI Impact of Temporal Autocorrelation Mismatch on the Assimilation of Satellite-Derived Surface Soil Moisture Retrievals SO IEEE JOURNAL OF SELECTED TOPICS IN APPLIED EARTH OBSERVATIONS AND REMOTE SENSING LA English DT Article DE Data assimilation; ecohydrological model; multitemporal time series analysis; soil moisture ID NORTH CHINA PLAIN; ECO-HYDROLOGICAL MODEL; ENSEMBLE KALMAN FILTER; ECOSYSTEM MODEL; EVAPOTRANSPIRATION; BASIN; WHEAT AB Satellite-based surface soil moisture retrievals are commonly assimilated into ecohydrological models in order to obtain improved profile soil moisture estimates. However, differences in temporal autocorrelation structure between these retrievals and comparable model-based predictions can potentially undermine the efficiency of such assimilation. Here, we conduct a series of synthetic experiments to examine the magnitude of this problem and the potential for detecting the presence of retrieval/model autocorrelation differences using a simple diagnostic procedure. Our synthetic experiments are based on modifying the observation operator within a data assimilation system to artificially induce differences in temporal autocorrelation between assimilated surface soil moisture retrievals and comparable surface soil moisture estimates made by an off-line ecohydrological assimilation model. Results demonstrate that neglecting a mismatch in retrieval/assimilation model autocorrelation can reduce the benefit of surface soil moisture data assimilation. The impact is especially large for soil profiles with limited vertical coupling. However, the presence of this source of retrieval/model autocorrelation misfit is detectable using a simple diagnostic index derived from a time series of soil moisture retrievals and open loop model predictions. Using relatively short data sets (similar to 2 years in length), the diagnostic is capable of identifying worst-case scenarios leading to the most significant degradation of assimilation results. C1 [Qiu, Jianxiu; Crow, Wade T.] ARS, Hydrol & Remote Sensing Lab, USDA, Beltsville, MD 20705 USA. [Qiu, Jianxiu; Mo, Xingguo; Liu, Suxia] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Water Cycle & Related Land Surface Proc, Beijing 100101, Peoples R China. RP Qiu, JX (reprint author), Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Water Cycle & Related Land Surface Proc, Beijing 100101, Peoples R China. EM qiujx.10b@igsnrr.ac.cn; wade.crow@ars.usda.gov; moxg@igsnrr.ac.cn; liusx@igsnrr.ac.cn RI Qiu, Jianxiu/O-6255-2016 OI Qiu, Jianxiu/0000-0002-1275-4171 FU Natural Science Foundation of China [31171451]; Key Project for the Strategic Science Plan in IGSNRR, CAS [2012ZD003]; Chinese Ministry of Science and Technology for "973" [2010CB428404]; China Scholarship Council (CSC) [201204910274] FX This work was supported in part by the project of Natural Science Foundation of China under Grant 31171451, in part by the Key Project for the Strategic Science Plan in IGSNRR, CAS under Grant 2012ZD003, in part by the Chinese Ministry of Science and Technology for "973" project under Grant 2010CB428404. The work of W. T. Crow was supported by NASA Water Resources Applied Science Grant, and the work of J. Qiu was supported by China Scholarship Council (CSC) under Grant 201204910274. NR 25 TC 1 Z9 1 U1 1 U2 9 PU IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC PI PISCATAWAY PA 445 HOES LANE, PISCATAWAY, NJ 08855-4141 USA SN 1939-1404 EI 2151-1535 J9 IEEE J-STARS JI IEEE J. Sel. Top. Appl. Earth Observ. Remote Sens. PD AUG PY 2014 VL 7 IS 8 BP 3534 EP 3542 DI 10.1109/JSTARS.2014.2349354 PG 9 WC Engineering, Electrical & Electronic; Geography, Physical; Remote Sensing; Imaging Science & Photographic Technology SC Engineering; Physical Geography; Remote Sensing; Imaging Science & Photographic Technology GA AQ8DZ UT WOS:000343055200032 ER PT J AU Malone, SL Staudhammer, CL Loescher, HW Olivas, P Oberbauer, SF Ryan, MG Schedlbauer, J Starr, G AF Malone, Sparkle L. Staudhammer, Christina L. Loescher, Henry W. Olivas, Paulo Oberbauer, Steven F. Ryan, Michael G. Schedlbauer, Jessica Starr, Gregory TI Seasonal patterns in energy partitioning of two freshwater marsh ecosystems in the Florida Everglades SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE Florida Everglades; subtropical wetlands; surface energy balance; energy partitioning; time series analysis; eddy covariance ID SENSIBLE HEAT-FLUX; PRAIRIE WETLAND; EDDY-COVARIANCE; CARBON-DIOXIDE; SOUTH FLORIDA; PLANT ZONATION; SALT MARSHES; TYPHA MARSH; WARM-SEASON; EVAPOTRANSPIRATION AB We analyzed energy partitioning in short- and long-hydroperiod freshwater marsh ecosystems in the Florida Everglades by examining energy balance components (eddy covariance derived latent energy (LE) and sensible heat (H) flux). The study period included several wet and dry seasons and variable water levels, allowing us to gain better mechanistic information about the control of and changes in marsh hydroperiods. The annual length of inundation is similar to 5 months at the short-hydroperiod site (25 degrees 2616.5N, 80 degrees 3540.68W), whereas the long-hydroperiod site (25 degrees 336.72N, 80 degrees 4657.36W) is inundated for similar to 12 months annually due to differences in elevation and exposure to surface flow. In the Everglades, surface fluxes feed back to wet season precipitation and affect the magnitude of seasonal change in water levels through water loss as LE (evapotranspiration (ET)). At both sites, annual precipitation was higher than ET (1304 versus 1008 at the short-hydroperiod site and 1207 versus 1115 mm yr(-1) at the long-hydroperiod site), though there were seasonal differences in the ratio of ET:precipitation. Results also show that energy balance closure was within the range found at other wetland sites (60 to 80%) and was lower when sites were inundated (60 to 70%). Patterns in energy partitioning covaried with hydroperiods and climate, suggesting that shifts in any of these components could disrupt current water and biogeochemical cycles throughout the Everglades region. These results suggest that the complex relationships between hydroperiods, energy exchange, and climate are important for creating conditions sufficient to maintain Everglades ecosystems. C1 [Malone, Sparkle L.; Staudhammer, Christina L.; Starr, Gregory] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Malone, Sparkle L.; Ryan, Michael G.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. [Loescher, Henry W.] Natl Ecol Observ Network Inc, Boulder, CO USA. [Loescher, Henry W.] Univ Colorado Boulder, Inst Arctic & Alpine Res, Boulder, CO USA. [Olivas, Paulo; Oberbauer, Steven F.; Schedlbauer, Jessica] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA. [Ryan, Michael G.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Schedlbauer, Jessica] West Chester Univ Penn, Dept Biol, W Chester, PA USA. RP Malone, SL (reprint author), Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. EM slmalone@crimson.ua.edu RI Ryan, Michael/A-9805-2008 OI Ryan, Michael/0000-0002-2500-6738 FU Department of Energy's (DOE) National Institute for Climate Change Research (NICCR) [07-SC-NICCR-1059]; U.S. Department of Education Graduate Assistantships in Areas of National Need (GAANN) program; Florida International University; National Science Foundation (NSF) Division of Atmospheric and Geospace Sciences [1233006]; NSF through the Florida Coastal Everglades Long-Term Ecological Research program [DBI-0620409, DEB-9910514]; United States Forest Service Rocky Mountain Research Station; NSF [EF-102980]; NEON, Inc. FX All research was performed under permits issued by Everglades National Park (EVER-2009-SCI-0070 and EVER-2013-SCI-0058) and the flux data for TS and SRS are made available through AmeriFlux (http://ameriflux.ornl.gov). This research is based in part on support of the Department of Energy's (DOE) National Institute for Climate Change Research (NICCR) through grant 07-SC-NICCR-1059, the U.S. Department of Education Graduate Assistantships in Areas of National Need (GAANN) program, Florida International University, and the National Science Foundation (NSF) Division of Atmospheric and Geospace Sciences (AGS), Atmospheric Chemistry program through grant 1233006. This research was also supported by the NSF through the Florida Coastal Everglades Long-Term Ecological Research program under Cooperative Agreements DBI-0620409 and DEB-9910514 and by the United States Forest Service Rocky Mountain Research Station. H. W. L. acknowledges the NSF, EF-102980, for their ongoing support. The National Ecological Observatory Network (NEON) is a project sponsored by the NSF and managed under cooperative agreement by NEON, Inc. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the NSF. Finally, the authors would like to recognize all those that have advanced our predictive understanding of ecology, past, present, and in the future. NR 99 TC 5 Z9 5 U1 1 U2 21 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD AUG PY 2014 VL 119 IS 8 BP 1487 EP 1505 DI 10.1002/2014JG002700 PG 19 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AQ7KK UT WOS:000342993200002 ER PT J AU Morse, JL Werner, SF Gillin, CP Goodale, CL Bailey, SW McGuire, KJ Groffman, PM AF Morse, J. L. Werner, S. F. Gillin, C. P. Goodale, C. L. Bailey, S. W. McGuire, K. J. Groffman, P. M. TI Searching for biogeochemical hot spots in three dimensions: Soil C and N cycling in hydropedologic settings in a northern hardwood forest SO JOURNAL OF GEOPHYSICAL RESEARCH-BIOGEOSCIENCES LA English DT Article DE N mineralization; nitrification; denitrification; microbial respiration; soil profile ID RIPARIAN ZONES; SHALLOW GROUNDWATER; LANDSCAPE POSITION; ORGANIC-CARBON; NITROGEN; DENITRIFICATION; ECOSYSTEM; TERRESTRIAL; CATCHMENT; HYDROLOGY AB Understanding and predicting the extent, location, and function of biogeochemical hot spots at the watershed scale is a frontier in environmental science. We applied a hydropedologic approach to identify (1) biogeochemical differences among morphologically distinct hydropedologic settings and (2) hot spots of microbial carbon (C) and nitrogen (N) cycling activity in a northern hardwood forest in Hubbard Brook Experimental Forest, New Hampshire, USA. We assessed variables related to C and N cycling in spodic hydropedologic settings (typical podzols, bimodal podzols, and Bh podzols) and groundwater seeps during August 2010. We found that soil horizons (Oi/Oe, Oa/A, and B) differed significantly for most variables. B horizons (>10cm) accounted for 71% (11%) of C pools and 62% (10%) of microbial biomass C in the sampled soil profile, whereas the surface horizons (Oi/Oe and Oa/A; 0-10cm) were dominant zones for N-cycle-related variables. Watershed-wide estimates of C and N cycling were higher by 34 to 43% (17-19%) when rates, horizon thickness, and areal extent of each hydropedologic setting were incorporated, versus conventionally calculated estimates for typical podzols that included only the top 10cm of mineral soil. Despite the variation in profile development in typical, bimodal, and Bh podzols, we did not detect significant differences in C and N cycling among them. Across all soil horizons and hydropedologic settings, we found strong links between biogeochemical cycling and soil C, suggesting that the accumulation of C in soils may be a robust indicator of microbial C and N cycling capacity in the landscape. C1 [Morse, J. L.; Groffman, P. M.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. [Morse, J. L.] Portland State Univ, Dept Environm Sci & Management, Portland, OR 97207 USA. [Werner, S. F.] Syracuse Univ, Dept Civil & Environm Engn, Syracuse, NY 13244 USA. [Gillin, C. P.] Virginia Tech, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. [Goodale, C. L.] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY USA. [Bailey, S. W.] US Forest Serv, No Res Stn, North Woodstock, NH USA. [McGuire, K. J.] Virginia Tech, Virginia Water Resources Res Ctr, Blacksburg, VA USA. RP Morse, JL (reprint author), Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. EM jlmorse@pdx.edu RI McGuire, Kevin/E-7770-2010; OI McGuire, Kevin/0000-0001-5751-3956; Bailey, Scott/0000-0002-9160-156X; Morse, Jennifer/0000-0001-8872-4940 FU U.S. National Science Foundation [DEB 0919047, DEB 0423259, EAR 1014507, DBI/EAR 0754678] FX We thank Lisa Martel, Robin Schmidt, Maggie Zimmer, and Maggie Burns for their help in the laboratory and field. We thank two anonymous reviewers for their useful input in revising this manuscript. This research was supported by the U.S. National Science Foundation grants (DEB 0919047-Ecosystem Studies, DEB 0423259-Hubbard Brook Long Term Ecological Research, EAR 1014507 and DBI/EAR 0754678-the Hubbard Brook REU program) and conducted at the Hubbard Brook Experimental Forest, which is operated by the Northern Research Station, USDA Forest Service, Newtown Square, PA. This article is a contribution to the Hubbard Brook Ecosystem Study. NR 51 TC 5 Z9 6 U1 3 U2 21 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 2169-8953 EI 2169-8961 J9 J GEOPHYS RES-BIOGEO JI J. Geophys. Res.-Biogeosci. PD AUG PY 2014 VL 119 IS 8 BP 1596 EP 1607 DI 10.1002/2013JG002589 PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AQ7KK UT WOS:000342993200008 ER PT J AU Sanderlin, JS Block, WM Ganey, JL AF Sanderlin, Jamie S. Block, William M. Ganey, Joseph L. TI Optimizing study design for multi-species avian monitoring programmes SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE avian community; Bayesian hierarchical model; cost-efficient; detection probability; occupancy; rare species; simulation study; species richness ID ESTIMATING SPECIES RICHNESS; ESTIMATING SITE OCCUPANCY; DETECTION PROBABILITY; IMPERFECT DETECTION; SAMPLING DESIGN; MODELS; CONSERVATION; DETECTABILITY; COMMUNITIES; ABUNDANCE AB 1. Many monitoring programmes are successful at monitoring common species, whereas rare species, which are often of highest conservation concern, may be detected infrequently. Study designs that increase the probability of detecting rare species at least once over the study period, while collecting adequate data on common species, strengthen programme ability to address community-wide hypotheses about how an ecosystem functions or responds to management actions. Study design guidelines exist for single-species occupancy models, but practical guidance for monitoring species communities is needed. Single-species population-level designs are necessarily optimal for targeted species, whereas community study designs may be optimal for the assemblage of species, but not for every species within the community. Our objective was to provide a general optimization tool for multi-species models and to illustrate this tool using data from two avian community studies. 2. We conducted a simulation study with a Bayesian hierarchical model to explore design and cost trade-offs for avian community monitoring programmes. We parameterized models using two long-term avian studies from Arizona, USA and evaluated bias and accuracy for different combinations of species in the regional species pool and sampling design combinations of number of sites and sampling occasions. We optimized for maximum accuracy of species richness, detection probability and occupancy probability of rare species, given a fixed budget. 3. Statistical properties for species richness and detection probability of species within the community were driven more by sampling occasions, whereas rare species occupancy probability was influenced more by percentage of area sampled. These results are consistent with results from single-species models, suggesting some similarities between community-level and single-species models with occupancy and detection. 4. Synthesis and applications. Study designs must be cost-efficient while providing reliable knowledge. Researchers and managers have limited funds for collecting data. Our template can be used by researchers and managers to evaluate trade-offs and efficiencies for allocating samples between the number of occasions and sites in multi-species studies, and allow them to identify the optimal study design in order to achieve specific study objectives. C1 [Sanderlin, Jamie S.; Block, William M.; Ganey, Joseph L.] US Forest Serv, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Sanderlin, JS (reprint author), US Forest Serv, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM jlsanderlin@fs.fed.us FU National Fire Plan FX National Fire Plan supported J.S.S. We thank J.A. Royle for helpful analysis discussions, field crews, and Coronado National Forest, Coconino National Forest, The Nature Conservancy, Fort Huachuca, Santa Rita Experimental Range and Audubon Research Ranch for logistical assistance. B. J. Bird reviewed statistical methods, and K. McKelvey, V. Ruiz-Gutierrez and three anonymous reviewers provided helpful reviews on manuscript versions. NR 36 TC 6 Z9 6 U1 7 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD AUG PY 2014 VL 51 IS 4 BP 860 EP 870 DI 10.1111/1365-2664.12252 PG 11 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AQ5LP UT WOS:000342851000004 ER PT J AU Byers, JA Naranjo, SE AF Byers, John A. Naranjo, Steven E. TI Detection and monitoring of pink bollworm moths and invasive insects using pheromone traps and encounter rate models SO JOURNAL OF APPLIED ECOLOGY LA English DT Article DE computer simulation; effective attraction radius; integrated pest management; invasive species; moth pheromones; Poisson distribution; predator-prey encounter equations ID EFFECTIVE ATTRACTION RADIUS; GOSSYPIELLA SAUNDERS LEPIDOPTERA; PECTINOPHORA-GOSSYPIELLA; FLYING INSECTS; SEX-PHEROMONE; BAITED TRAPS; COMPETITIVE ATTRACTION; PEST-MANAGEMENT; UNITED-STATES; BT COTTON AB 1. The pink bollworm moth Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae) is one of the most destructive pests in agriculture. An ongoing eradication program using a combination of sex pheromone monitoring and mating disruption, irradiated sterile moth releases, genetically modified Bt cotton and local insecticide applications have all but exterminated the pink bollworm from the south-western USA and portions of northern Mexico. However, the continued threat of reinvasion from Mexico reinforces the need to improve pheromone-based monitoring. Invasions from other parts of the world such as India, where resistance to single-gene transgenic Bt cotton has evolved, further heightens the need for better monitoring strategies. 2. The mean flight height and standard deviation (SD) of the vertical flight distribution of pink bollworm males were estimated from catches on transparent sticky cylinder traps baited with synthetic pheromone at several heights above-ground. An effective attraction radius (EAR) of a standard pheromone lure was estimated from male moth catches on the pheromone-baited sticky traps and many similar blank traps. The circular EAR(c) was estimated from the spherical EAR and SD. 3. The EAR of a pheromone lure for pink bollworm was 1.03 m, and the EAR(c) was 2.61 m. The mean flight height of males was 0.82 m, and the vertical flight distribution SD was 0.26 m. 4. A computer program simulated male moth movement and capture on various numbers of traps of EAR(c) distributed over areas of 1-100 km(2). The simulated catch results were comparable to predictions using the EAR(c) with modified encounter rate equations of Royama and Rogers. The encounter rate equations were solved for initial populations of male moths in the regions, and Poisson statistics were used to calculate population confidence limits. 5. Synthesis and applications. Encounter rate models and Poisson methods can be used to determine levels of pheromone trap densities that are likely to detect and estimate low population levels of resident or invasive pink bollworms and many other pest insect species. C1 [Byers, John A.; Naranjo, Steven E.] USDA ARS, US Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Byers, JA (reprint author), USDA ARS, US Arid Land Agr Res Ctr, 21881 N Cardon Lane, Maricopa, AZ 85138 USA. EM John.Byers@ars.usda.gov NR 65 TC 4 Z9 4 U1 4 U2 30 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8901 EI 1365-2664 J9 J APPL ECOL JI J. Appl. Ecol. PD AUG PY 2014 VL 51 IS 4 BP 1041 EP 1049 DI 10.1111/1365-2664.12270 PG 9 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AQ5LP UT WOS:000342851000023 ER PT J AU Saponari, M Loconsole, G Cornara, D Yokomi, RK De Stradis, A Boscia, D Bosco, D Martelli, GP Krugner, R Porcelli, F AF Saponari, Maria Loconsole, Giuliana Cornara, Daniele Yokomi, Raymond K. De Stradis, Angelo Boscia, Donato Bosco, Domenico Martelli, Giovanni P. Krugner, Rodrigo Porcelli, Francesco TI Infectivity and Transmission of Xylella fastidiosa by Philaenus spumarius (Hemiptera: Aphrophoridae) in Apulia, Italy SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE spittlebug; vector transmission; xylem-fluid feeding insect; olive ID PIERCES DISEASE; LEAF SCORCH; VECTORS; PATHOGENICITY; CULTIVATION; GRAPEVINES; SEQUENCES; TAIWAN; ALMOND AB Discovery of Xylella fastidiosa from olive trees with "Olive quick decline syndrome" in October 2013 on the west coast of the Salento Peninsula prompted an immediate search for insect vectors of the bacterium. The dominant xylem-fluid feeding hemipteran collected in olive orchards during a 3-mo survey was the meadow spittlebug, Philaenus spumarius (L.) (Hemiptera: Aphrophoridae). Adult P. spumarius, collected in November 2013 from ground vegetation in X. fastidiosa-infected olive orchards, were 67% (40 out of 60) positive for X. fastidiosa by polymerase chain reaction (PCR) assays. Euscelis lineolatus Brulle were also collected but tested negative for the pathogen. Transmission tests with P. spumarius collected from the Salento area were, therefore, conducted. After a 96-h inoculation access period with 8 to 10 insects per plant and a 30-d incubation period, PCR results showed P. spumarius transmitted X. fastidiosa to two of five periwinkle plants but not to the seven olive plants. Sequences of PCR products from infected periwinkle were identical with those from X. fastidiosa-infected field trees. These data showed P. spumarius as a vector of X. fastidiosa strain infecting olives trees in the Salento Peninsula, Italy. C1 [Saponari, Maria; Loconsole, Giuliana; De Stradis, Angelo; Boscia, Donato] CNRS, Unita Org Bari, Ist Virol Vegetale, I-70126 Bari, Italy. [Cornara, Daniele; Martelli, Giovanni P.; Porcelli, Francesco] Univ Bari Aldo Moro, Dipartimento Sci Suolo Pianta & Alimenti, I-70126 Bari, Italy. [Yokomi, Raymond K.; Krugner, Rodrigo] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Bosco, Domenico] Univ Torino, Dipartimento Sci Agr Forestali & Alimentari, I-10095 Grugliasco, TO, Italy. RP Porcelli, F (reprint author), Univ Bari Aldo Moro, Dipartimento Sci Suolo Pianta & Alimenti, Via Amendola 165-A, I-70126 Bari, Italy. EM francesco.porcelli@uniba.it RI DE STRADIS, ANGELO/B-2969-2013; OI Francesco, Porcelli/0000-0001-5620-7826; SAPONARI, MARIA/0000-0001-9195-4624; BOSCIA, DONATO/0000-0002-4544-8680; BOSCO, Domenico/0000-0003-3945-2752 FU Regional Plant Health Service of Apulia FX This research was supported by grants from the Regional Plant Health Service of Apulia. We thank E. Manni (A. C. L. I. Racale Societa Agricola Cooperativa) and G. Altamura (Centro di Ricerca, Sperimentazione e Formazione in Agricoltura Basile Caramia, Locorotondo) for technical support. The authors would like to thank the two anonymous reviewers for their valuable comments and suggestions to improve the quality of the paper. NR 19 TC 16 Z9 19 U1 1 U2 52 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1316 EP 1319 DI 10.1603/EC14142 PG 4 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000003 PM 25195417 ER PT J AU Watt, TJ Duan, JJ AF Watt, Timothy J. Duan, Jian J. TI Influence of Host Age on Critical Fitness Parameters of Spathius galinae (Hymenoptera: Braconidae), a New Parasitoid of the Emerald Ash Borer (Coleoptera: Buprestidae) SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE invasive pest; Agrilus planipennis; biological control; parasitoid; fitness ID RUSSIAN FAR-EAST; SEX-RATIO; INTRASPECIFIC COMPETITION; APHYTIS-LINGNANENSIS; BIOLOGICAL-CONTROL; BIOTIC FACTORS; CLUTCH SIZE; EULOPHIDAE; EGG; ALLOCATION AB Spathius galinae Belokobylskij and Strazenac (Hymenoptera: Braconidae) is a recently discovered gregarious idiobiont larval ectoparasitoid currently being evaluated for biological control against the invasive emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) in the United States. To aid in the development of laboratory rearing protocols, we assessed the inauence of various emerald ash borer stages on critical fitness parameters of S. galinae. We exposed gravid S. galinae females to emerald ash borer host larvae of various ages (3.5, 5, 7, and 10 wk post egg oviposition) that were reared naturally in tropical (evergreen) ash (Fraxinus uhdei (Wenzig) Lingelsh) logs, or to field-collected, late-stage emerald ash borers (nonfeeding J-shaped larvae termed "J-larvae," prepupae, and pupae) that were artificially inserted into green ash logs. When exposed to larvae in tropical ash logs, S. galinae attacked 5 and 7 wk hosts more frequently (68-76%) than 3.5 wk (23%) and 10 wk (12%) hosts. Subsample dissections of the these logs revealed that 3.5, 5, 7 and 10 wk host logs contained mostly second, third, fourth, and J-larvae, respectively, that had already bored into the sapwood for diapause. No J-larvae were attacked by S. galinaewhennaturally reared in tropical ash logs. When parasitized by S. galinae, 7 and 10 wk hosts produced the largest broods (approximate to 6.7 offspring per parasitized host), and the progenies that emerged from these logs had larger anatomical measurements and more female-biased sex ratios. When exposed to emerald ash borer J-larvae, prepupae, or pupae artificially inserted into green ash logs, S. galinae attacked 53% of J-larvae, but did not attack any prepupae or pupae. We conclude that large (fourth instar) emerald ash borer larvae should be used to rear S. galinae. C1 [Watt, Timothy J.] Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA. [Watt, Timothy J.; Duan, Jian J.] ARS, USDA, Beneficial Insects Introduct Res Unit, Newark, DE 19713 USA. RP Watt, TJ (reprint author), Univ Delaware, Dept Entomol & Wildlife Ecol, Newark, DE 19713 USA. EM tjwatt@udel.edu FU U.S. Department of Agriculture-Agricultural Research Service [USDAD-ARS] [58-1926-2-282] FX We thank Kristi Larson, Jackie Hoban, and Sue Barth (all University of Delaware) for their assistance in maintaining laboratory insect cultures. We are grateful to Dick Bean, Kim Rice, Steve Bell, and Charles Pickett (all Maryland Department of Agriculture, Cheltenham, MD) for assistance in collecting logs and emerald ash borer from the field. We also thank Douglas Tallamy and Judith Hough-Goldstein for their assistance in the experimental design and helpful comments on the manuscript, and Dave Jennings for helpful comments on early versions of the manuscript. We are grateful to Doug Luster (U.S. Department of Agriculture-Agricultural Research Service [USDAD-ARS]) for reviewing the manuscript. This research was supported through funding from USDAD-APHIS grant 58-1926-2-282. NR 35 TC 4 Z9 5 U1 3 U2 19 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1320 EP 1329 DI 10.1603/EC14081 PG 10 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000004 PM 25195418 ER PT J AU Unahawutti, U Intarakumheng, R Oonthonglang, P Phankum, S Follett, PA AF Unahawutti, Udorn Intarakumheng, Rachada Oonthonglang, Pitawat Phankum, Salukjit Follett, Peter A. TI Nonhost Status of Mangosteen to Bactrocera dorsalis and Bactrocera carambolae (Diptera: Tephritidae) in Thailand SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE host status; Garcinia mangostana; quarantine pest; phytosanitary treatment; nonhost ID FRUIT-FLY DIPTERA; CONFIDENCE-LIMITS; SAMPLE-SIZE; HOST STATUS AB Postharvest quarantine treatments (irradiation or vapor heat) are used to control fruit flies and other pests in mangosteen (Garcinia mangostana L) exported to the United States and Japan from Thailand. No-choice tests were conducted in the laboratory to determine whether Thai mangosteen is a host for Bactrocera dorsalis (Hendel) (oriental fruit fly) and Bactrocera carambolae Drew & Hancock (carambola fruit fly). Ripe commercial quality fruit (1 wk after harvest) that were either undamaged or damaged by puncturing or peeling the pericarp were exposed to a high density of gravid flies in screen cages and then held for 10 d and dissected to inspect for immature life stages. Undamaged mangosteen fruit were not infested by B. dorsalis and B. carambolae. Partially damaged fruit with shallow punctures in the pericarp that did not extend to the aril also were not infested. Both fruit flies could infest damaged fruit if the pericarp damage allowed oviposition in the aril. Results suggest that natural infestation of mangosteen by B. dorsalis and B. carambolae can only occur if fruit exhibit physical cracks or mechanical injury. Resistance appears to be due to the pericarp hardness and thickness as well as latex secretion. Nonhost status could be used without additional quarantine measures to achieve quarantine security against B. dorsalis and B. carambolae in mangosteen exported from Thailand. C1 [Unahawutti, Udorn; Intarakumheng, Rachada; Oonthonglang, Pitawat; Phankum, Salukjit] Plant Protect Res & Dev Off, Dept Agr, Bangkok 10900, Thailand. [Follett, Peter A.] ARS, USDA, US Pacific Basin Agr Res Ctr, Hilo, HI 96720 USA. RP Follett, PA (reprint author), ARS, USDA, US Pacific Basin Agr Res Ctr, 64 Nowelo St, Hilo, HI 96720 USA. EM peter.follett@ars.usda.gov NR 18 TC 0 Z9 0 U1 2 U2 13 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1355 EP 1361 DI 10.1603/EC13566 PG 7 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000008 PM 25195422 ER PT J AU Vargas, RI Souder, SK Hoffman, K Mercogliano, J Smith, TR Hammond, J Davis, BJ Brodie, M Dripps, JE AF Vargas, Roger I. Souder, Steven K. Hoffman, Kevin Mercogliano, Juan Smith, Trevor R. Hammond, Jack Davis, Bobbie J. Brodie, Matt Dripps, James E. TI Attraction and Mortality of Bactrocera dorsalis (Diptera: Tephritidae) to STATIC Spinosad ME Weathered Under Operational Conditions in California and Florida: A Reduced-Risk Male Annihilation Treatment SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Oriental fruit fly; suppression; Min-U-Gel ME naled; MAT; alternative treatment ID ORIENTAL FRUIT-FLIES; METHYL EUGENOL; CUE-LURE; MELON FLIES; INVADENS DIPTERA; HENDEL DIPTERA; HAWAII; FLY; SPLAT; KILL AB Studies were conducted in 2013-2014 to quantify attraction, feeding, and mortality of male oriental fruit flies, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), to STATIC Spinosad ME a reduced-risk male annihilation treatment (MAT) formulation consisting of an amorphous polymer matrix in combination with methyl eugenol (ME) and spinosad compared with the standard treatment of Min-U-Gel mixed with ME and naled (Dibrom). Our approach used a behavioral methodology for evaluation of slow-acting reduced-risk insecticides. ME treatments were weathered for 1, 7, 14, 21, and 28 d under operational conditions in California and Florida and shipped to Hawaii for bioassays. In field tests using bucket traps to attract and capture wild males, and in toxicity studies conducted in 1-m 3 cages using released males of controlled ages, STATIC Spinosad ME performed equally as well to the standard formulation of Min-U-GelME with naled for material aged up to 28 d in both California and Florida. In laboratory feeding tests in which individual males were exposed for 5 min to the different ME treatments, mortality induced by STATIC Spinosad ME recorded at 24 h did not differ from mortality caused by Min-U-Gel ME with naled at 1, 7, 14, and 21 d in California and was equal to or higher for all weathered time periods in Florida during two trials. Spinosad has low contact toxicity, and when mixed with an attractant and slow release matrix, offers a reduced-risk alternative for eradication of B. dorsalis and related ME attracted species, without many of the potential negative effects to humans and nontargets associated with broad-spectrum contact insecticides such as naled. C1 [Vargas, Roger I.; Souder, Steven K.] ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, TCCPRU, USDA, Hilo, HI 96720 USA. [Hoffman, Kevin; Mercogliano, Juan] Calif Dept Food & Agr, Sacramento, CA 95814 USA. [Smith, Trevor R.; Hammond, Jack; Davis, Bobbie J.; Brodie, Matt] Florida Dept Agr & Consumer Serv, Div Plant Ind, Gainesville, FL 32608 USA. [Dripps, James E.] Dow AgroSci LLC, Crop Protect R&D, Indianapolis, IN 46268 USA. RP Vargas, RI (reprint author), ARS, Daniel K Inouye US Pacific Basin Agr Res Ctr, TCCPRU, USDA, 64 Nowelo St, Hilo, HI 96720 USA. EM roger.vargas@ars.usda.gov FU CDFA (Sacramento, CA) FX We thank Russell Ijima, Jordan "Kama" Lee Loy, and Daisy L. Willis (Pacific Basin Agricultural Research Center, USDAD ARS, Hilo, HI) for experimental setup and data collection throughout these trials. We thank James Mueller and Alejandro Calixto (DowAgroSciences) for technical advice and assistance in applying the treatments. In addition, we thank Synda Boulahia-Kheder (National Institute of Agronomy, Cite Mahrajene, Tunisia) and Ming-Yi Chou (University of Hawaii at Manoa, Honolulu, HI) for coordinating feeding trials. We thank W. H. Shipman Limited, A & B Properties, and Kauai Coffee Plantation, and in particular, Randall S. Akao, Greg Williams, and various papaya growers for allowing access to field sites for field attraction evaluations. We also thank Patrick J. Gomesand John C. Stewart of the Animal and Plant Health Inspection Service (APHIS) for their support and guidance. We appreciate the advice and input of Earl Andress (APHIS, Los Alamitos, CA) for the field trials. Without the financial support of CDFA (Sacramento, CA) these trials could not have been completed. We also recognize the contributions of Agenor Mafra-Neto in formulating and manufacturing the spinosad test product (ISCA Technologies, Riverside, CA). IR-4 funds were essential for planning and completing this research. Finally, we appreciate the comments by John Stark, Luc Leblanc, Robert Dowell, and Nicholas Manoukis on an earlier version of this manuscript. Mention of a proprietary product does not constitute an endorsement or a recommendation by the USDA for its use. USDA is an equal opportunity provider and employer. NR 34 TC 1 Z9 2 U1 3 U2 27 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1362 EP 1369 DI 10.1603/EC14121 PG 8 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000009 PM 25195423 ER PT J AU Liu, SS Liu, YB Simmons, GS AF Liu, Samuel S. Liu, Yong-Biao Simmons, Gregory S. TI Oxygenated Phosphine Fumigation for Control of Epiphyas postvittana (Lepidoptera: Tortricidae) Eggs on Lettuce SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE phosphine; fumigation; Lactuca sativa; light brown apple moth; phytosanitary ID BROWN APPLE MOTH; TOXICITY AB Light brown apple moth, Epiphyas postvittana (Walker), is a quarantined pest in most countries. Its establishment in California and potential spread to other parts of the state and beyond make it urgent to develop effective postharvest treatments to control the pest on fresh commodities. Fumigation with cylindered phosphine at low temperature has emerged to be a practical methyl bromide alternative treatment for postharvest pest control on fresh commodities. However, its use to control E. postvittana eggs on sensitive commodities such as lettuce is problematic. E. postvittana eggs are tolerant of phosphine and long phosphine treatment also injures lettuce. In the current study, E. postvittana eggs were subjected to oxygenated phosphine fumigations to develop an effective treatment at a low storage temperature of 2 degrees C. In addition, soda lime as a CO2 absorbent was tested to determine its effects in reducing and preventing injuries to lettuce associated with phosphine fumigations. Three-day fumigation with 1,000 ppm phosphine under 60% O-2 achieved 100% mortality of E. postvittana eggs in small-scale laboratory tests. In the presence of the CO2 absorbent, a 3-d large-scale fumigation of lettuce with 1,700 ppm phosphine under 60% O-2 resulted in a relative egg mortality of 99.96% without any negative effect on lettuce quality. The 3-d fumigation treatment without the CO2 absorbent, however, resulted in significant injuries to lettuce and consequential quality reductions. The study demonstrated that oxygenated phosphine fumigation has the potential to control E. postvittana eggs and the CO2 absorbent has the potential to prevent injuries and quality reductions of lettuce associated with long-term oxygenated phosphine fumigation. C1 [Liu, Samuel S.; Liu, Yong-Biao] ARS, USDA, Salinas, CA 93905 USA. [Simmons, Gregory S.] USDA, APHIS, PPQ, CPHST, Salinas, CA 93905 USA. RP Liu, YB (reprint author), ARS, USDA, 1636 E Alisal St, Salinas, CA 93905 USA. EM yongbiao.liu@ars.usda.gov FU U.S. Department of Agriculture, Foreign Agricultural Service FX We thank T. Greene, S. Kane, T. Masuda, and J. Wasson for technical assistance. We thank Tanimura & Antle Co. (Salinas, CA) for providing lettuce. This study was supported by a Technical Assistance for Specialty Crop program of U.S. Department of Agriculture, Foreign Agricultural Service. NR 24 TC 2 Z9 2 U1 1 U2 3 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1370 EP 1376 DI 10.1603/EC13487 PG 7 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000010 PM 25195424 ER PT J AU Hallman, GJ AF Hallman, Guy J. TI Insect Thermotolerance Comparing Host Infestation Methods: Anastrepha ludens (Diptera: Tephritidae) Reared in Grapefruit or Diet SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE heat treatment; quarantine treatment; phytosanitary treatment; Mexican fruit fly ID FRUIT-FLY DIPTERA; HOT-WATER IMMERSION; THERMAL DEATH; FLIES DIPTERA; MORTALITY; EFFICACY AB Research on insect control should be conducted in a manner that mimics as closely as is feasible its commercial application in all of its practicably conceivable forms. When significant deviations from commercial application are used in research, the effect of the deviations on efficacy should be evaluated. Pest control techniques are sometimes based on research that used untested assumptions about variables that might affect efficacy. For example, some phytosanitary treatments are based on research done with diet-reared larvae inserted into holes bored in fruits, although the effect of this manipulation has not been evaluated. This research compares this type of infestation of grapefruit with Mexican fruit fly, Anastrepha ludens (Loew), third instars with a more natural infestation technique whereby females were allowed to oviposit on picked grapefruit in laboratory cages and third instars were reared inside the fruit. Although the results did not show statistically significant differences between infestation techniques, tendencies in the data caution against researchers making assumptions about efficacy without testing them when experimental techniques stray from more natural situations for which the research is designed. C1 ARS, USDA, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA. RP Hallman, GJ (reprint author), ARS, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Ave, Manhattan, KS 66502 USA. EM Guy.Hallman@ars.usda.gov NR 22 TC 1 Z9 1 U1 0 U2 1 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1377 EP 1384 DI 10.1603/EC14111 PG 8 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000011 PM 25195425 ER PT J AU Xie, HC Zhao, L Wang, WQ Wang, ZY Ni, XZ Cai, WZ He, KL AF Xie, Haicui Zhao, Lei Wang, Wenqiang Wang, Zhenying Ni, Xinzhi Cai, Wanzhi He, Kanglai TI Changes in Life History Parameters of Rhopalosiphum maidis (Homoptera: Aphididae) Under Four Different Elevated Temperature and CO2 Combinations SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Rhopalosiphum maidis; life history; elevated temperature; CO2; development time ID ATMOSPHERIC CARBON-DIOXIDE; INSECT-PLANT INTERACTIONS; TRI-TROPHIC INTERACTION; CORN LEAF APHID; CLIMATE-CHANGE; POPULATION RESPONSES; GOSSYPIUM-HIRSUTUM; PERFORMANCE; HERBIVORY; ABUNDANCE AB Biological characteristics of corn leaf aphid, Rhopalosiphum maidis (Fitch), on barley, Hordeum vulgare L., were examined for two generations under four different elevated temperature and CO2 combinations. The developmental duration for each life stage was significantly reduced under the elevated temperature (+4 degrees C). The elevated CO2 (700-750 mu l/liter) reduced only the development time of fourth-instar nymph. The overall duration of nymphal stage was reduced in the second generation. Thus, the temperature was the dominant factor to development duration of corn leaf aphid. The fecundity of corn leaf aphid was significantly increased under the elevated temperature and CO2, as well as in the later generation. Elevated temperature and CO2 increased the number of alate production, which may enhance the aphid migration or dispersal and the spread of plant viruses. Corn leaf aphid had the highest intrinsic rate of increase under the elevated temperature and CO2 combination in the second generation. These results indicate that the combined effects of both elevated temperature and CO2 on aphid biology may exacerbate aphid damage on barley under the climate change in accompany with elevated temperature and CO2 level. C1 [Xie, Haicui; Zhao, Lei; Wang, Wenqiang; Wang, Zhenying; He, Kanglai] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. [Xie, Haicui; Cai, Wanzhi] China Agr Univ, Dept Entomol, Beijing 100094, Peoples R China. [Ni, Xinzhi] Univ Georgia, ARS, USDA, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. RP He, KL (reprint author), Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China. EM klhe@ippcaas.cn FU National 973 Program of the Ministry of Science and Technology of the People's Republic of China [2010CB951503] FX The funding for this research was supported by the National 973 Program of the Ministry of Science and Technology of the People's Republic of China (2010CB951503). We greatly appreciate the critical and constructive comments and advice from the reviewers on an earlier version of the manuscript. NR 41 TC 3 Z9 3 U1 3 U2 35 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1411 EP 1418 DI 10.1603/EC13302 PG 8 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000015 PM 25195429 ER PT J AU Nagoshi, RN Meagher, RL Hay-Roe, M AF Nagoshi, Rodney N. Meagher, Robert L. Hay-Roe, Mirian TI Assessing the Resolution of Haplotype Distributions to Delineate Fall Armyworm (Lepidoptera: Noctuidae) Migratory Behaviors SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Spodoptera frugiperda; host strains; migration ID SPODOPTERA-FRUGIPERDA LEPIDOPTERA; CORN-STRAIN POPULATIONS; UNITED-STATES; HOST STRAINS; FLORIDA; TEXAS; BRAZIL; IDENTIFICATION AB Regions of southern Florida and southern Texas (extending into Mexico) provide the overwintering source populations for virtually all fall armyworm infestations affecting the continental United States. Understanding how these migratory populations annually disperse is important to predict and control infestations by this specific pest and to more generally investigate the environmental factors that influence the long-distance movements of flying insects. The two overwintering locations are associated with differences in the distribution of certain mitochondrial haplotypes that overlap in the region near the border separating the states of Alabama and Georgia. This provided an opportunity to test the resolution of the haplotype method by comparisons between smaller geographical areas and shorter time frames than previously examined. Correspondences were found between trap-capture numbers, fall armyworm strain proportions, and haplotype ratios calculated for individual counties and within season time periods that were generally consistent with expectations, providing confidence that those population movements could be accurately inferred. The comparison of haplotype distributions identified a migratory boundary separating the Texas and Florida populations coincident with the eastern edge of the Apalachicola-Chattahoochee-Flint River basin. Calculations of strain numbers based on genetic markers revealed similarities and differences in strain population dynamics that can be applied to study the migratory behavior of fall armyworm subpopulations. The use of this methodology for the detailed mapping of migratory pathways and the identification of factors that influence the direction and extent of pest migration are discussed. C1 [Nagoshi, Rodney N.; Meagher, Robert L.; Hay-Roe, Mirian] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Hay-Roe, Mirian] Univ Florida, Everglades Res & Educ Ctr, Belle Glade, FL 33430 USA. RP Nagoshi, RN (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM rodney.nagoshi@ars.usda.gov FU National Institute of Food and Agriculture, U.S. Department of Agriculture [2011-67003-30209] FX We thank Kathy Flanders (Auburn University), and the regional and county extension agents and growers in Alabama and Georgia for assistance in locating sample sites. We thank Jane Sharp and Jean Thomas at U.S. Department of Agriculture-Agricultural Research Service (USDA-ARS) for excellent technical support and assistance. Weare grateful to Sandra Allan (USDA-ARS) and Steven Valles (USDA-ARS) for helpful comments on the manuscript. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the U.S. Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. This material is based upon [work] supported by the National Institute of Food and Agriculture, U.S. Department of Agriculture, under Agreement 2011-67003-30209. NR 22 TC 2 Z9 2 U1 1 U2 18 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1462 EP 1470 DI 10.1603/EC14124 PG 9 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000023 PM 25195437 ER PT J AU Crook, DJ Francese, JA Rietz, ML Lance, DR Hull-Sanders, HM Mastro, VC Silk, PJ Ryall, KL AF Crook, Damon J. Francese, Joseph A. Rietz, Michael L. Lance, David R. Hull-Sanders, Helen M. Mastro, Victor C. Silk, Peter J. Ryall, Krista L. TI Improving Detection Tools for Emerald Ash Borer (Coleoptera: Buprestidae): Comparison of Multifunnel Traps, Prism Traps, and Lure Types at Varying Population Densities SO JOURNAL OF ECONOMIC ENTOMOLOGY LA English DT Article DE Agrilus planipennis; sex pheromone; kairomone; multifunnel trap; fluon ID AGRILUS-PLANIPENNIS COLEOPTERA; CAPTURING CERAMBYCID BEETLES; PANEL TRAPS; COLOR; PLACEMENT; EFFICACY; PHEROMONE; (Z)-3-HEXENOL; OPTIMIZATION; ATTRACTION AB The emerald ash borer, Agrilus planipennis Fairmaire (Coleoptera: Buprestidae), is a serious invasive pest of North American ash (Fraxinus spp.) that has caused devastating mortality since it was first identified in North America in 2002. In 2012, we conducted field trapping assays that tested the efficacy of purple prism and fluon-coated green multifunnel (Lindgren funnel) traps. Traps were baited with combinations of several lures that were previously shown to be attractive to A. planipennis: manuka oil-a sesquiterpene-rich oil, (3Z)-hexenol-a green leaf volatile, or (3Z)-dodecen-12-olide [= (3Z)-lactone], a sex pheromone. Eighty-nine blocks (trap lines) were tested throughout nine states along the outer edges of the currently known A. planipennis infestation in North America. Trap catch was highest on fluon-coated green multifunnel traps, and trap detections at sites with low A. planipennis population density ranged from 72 to 76% for all trap and lure types tested. (3Z)-hexenol and (3Z)-lactone baited traps functioned as well as (3Z)-hexenol and manuka oil-baited traps. Independent of the lure used, detection rates on green fluon-coated multifunnel traps were comparable with glued purple prism traps in areas with low A. planipennis population densities. C1 [Crook, Damon J.; Francese, Joseph A.; Lance, David R.; Hull-Sanders, Helen M.; Mastro, Victor C.] USDA APHIS PPQ CPHST, Otis Lab, Buzzards Bay, MA 02542 USA. [Rietz, Michael L.] USDA APHIS PPQ, Emerald Ash Borer Project, Brighton, MI 48116 USA. [Silk, Peter J.] Nat Resources Canada, Atlantic Forestry Ctr, Fredericton, NB E3B 5P7, Canada. [Ryall, Krista L.] Nat Resources Canada, Great Lakes Forestry Ctr, Sault Ste Marie, ON P6A 2G3, Canada. RP Crook, DJ (reprint author), USDA APHIS PPQ CPHST, Otis Lab, 1398 West Truck Rd, Buzzards Bay, MA 02542 USA. EM damon.j.crook@aphis.usda.gov FU USDA-APHIS-PPQ Emerald Ash Borer Program; Canadian Forest Service; Forest Protection Ltd, Ontario, Manitoba; Saskatchewan Natural Resources, USDA-FS; Atlantic Innovation Fund-Atlantic Canada Opportunities Agency FX We would like to express our gratitude to the staff, especially Bethany Boegler and Ben Sorensen of the U.S. Department of Agriculture-Animal and Plant Health Inspection Service-Plant Protection and Quarantine-Center for Plant Health Science and Technology (USDA-APHIS-PPQ-CPHST) laboratory in Brighton, MI, for their assistance with fieldwork and data compilation. This study would not have been possible without the help of numerous state and USDA-APHIS-PPQ cooperators who helped with trap set-up and checking. We would also like to thank the two anonymous reviewers for helpful comments on the manuscript. This work was funded by the USDA-APHIS-PPQ Emerald Ash Borer Program. In Canada, funding came from, Canadian Forest Service, Forest Protection Ltd, Ontario, Manitoba and Saskatchewan Natural Resources, USDA-FS, and Atlantic Innovation Fund-Atlantic Canada Opportunities Agency. NR 37 TC 3 Z9 3 U1 0 U2 23 PU ENTOMOLOGICAL SOC AMER PI ANNAPOLIS PA 3 PARK PLACE, STE 307, ANNAPOLIS, MD 21401-3722 USA SN 0022-0493 EI 1938-291X J9 J ECON ENTOMOL JI J. Econ. Entomol. PD AUG PY 2014 VL 107 IS 4 BP 1496 EP 1501 DI 10.1603/EC14041 PG 6 WC Entomology SC Entomology GA AQ1TT UT WOS:000342565000027 PM 25195441 ER PT J AU Kool, D Ben-Gal, A Agam, N Simunek, J Heitman, JL Sauer, TJ Lazarovitch, N AF Kool, D. Ben-Gal, A. Agam, N. Simunek, J. Heitman, J. L. Sauer, T. J. Lazarovitch, N. TI Spatial and diurnal below canopy evaporation in a desert vineyard: Measurements and modeling SO WATER RESOURCES RESEARCH LA English DT Article DE evaporation; HYDRUS (2-D; 3-D); diurnal variability; spatial variability; evapotranspiration partitioning; potential evaporation ID ROOT WATER-UPTAKE; ROW-CROP CANOPY; DRIP IRRIGATION; ENERGY-BALANCE; EVAPOTRANSPIRATION COMPONENTS; MICROLYSIMETRY TECHNIQUES; SOIL EVAPORATION; SONIC ANEMOMETER; HEAT-FLUX; SAP FLOW AB Evaporation from the soil surface (E) can be a significant source of water loss in arid areas. In sparsely vegetated systems, E is expected to be a function of soil, climate, irrigation regime, precipitation patterns, and plant canopy development and will therefore change dynamically at both daily and seasonal time scales. The objectives of this research were to quantify E in an isolated, drip-irrigated vineyard in an arid environment and to simulate below canopy E using the HYDRUS (2-D/3-D) model. Specific focus was on variations of E both temporally and spatially across the inter-row. Continuous above canopy measurements, made in a commercial vineyard, included evapotranspiration, solar radiation, air temperature and humidity, and wind speed and direction. Short-term intensive measurements below the canopy included actual and potential E and solar radiation along transects between adjacent vine-rows. Potential and actual E below the canopy were highly variable, both diurnally and with distance from the vine-row, as a result of shading and distinct wetted areas typical to drip irrigation. While the magnitude of actual E was mostly determined by soil water content, diurnal patterns depended strongly on position relative to the vine-row due to variable shading patterns. HYDRUS (2-D/3-D) successfully simulated the magnitude, diurnal patterns, and spatial distribution of E, including expected deviations as a result of variability in soil saturated hydraulic conductivity. C1 [Kool, D.; Ben-Gal, A.] Agr Res Org, Gilat Res Ctr, Inst Soil Water & Environm Sci, Gilat, Israel. [Kool, D.; Agam, N.; Lazarovitch, N.] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Wyler Dept Dryland Agr, French Associates Inst Agr & Biotechnol Drylands, Midreshet Ben Gurion, Israel. [Simunek, J.] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA. [Heitman, J. L.] N Carolina State Univ, Dept Soil Sci, Raleigh, NC 27695 USA. [Sauer, T. J.] ARS, Natl Lab Agr & Environm, USDA, Ames, IA USA. RP Ben-Gal, A (reprint author), Agr Res Org, Gilat Res Ctr, Inst Soil Water & Environm Sci, Gilat, Israel. EM bengal@volcani.agri.gov.il RI Kool, Dilia/F-8273-2015 OI Kool, Dilia/0000-0002-8332-1429 FU BARD, the United States-Israel Binational Agricultural Research and Development Fund [US-4262-09]; I-CORE Program of the Planning and Budgeting Committee; Israel Science Foundation [152/11] FX This research was supported by Research Grant No. US-4262-09 from BARD, the United States-Israel Binational Agricultural Research and Development Fund, and was partially supported by the I-CORE Program of the Planning and Budgeting Committee and the Israel Science Foundation (grant no. 152/11). We are particularly grateful for technical and field support from the Gilat Research Center's Eugene Presnov and the Jacob Blaustein Institutes for Desert Research's Yuval Shani. We also thank Yoav Rabani for graciously providing his vineyard as an experimental site. NR 70 TC 8 Z9 9 U1 2 U2 32 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD AUG PY 2014 VL 50 IS 8 BP 7035 EP 7049 DI 10.1002/2014WR015409 PG 15 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AQ2QJ UT WOS:000342632300045 ER PT J AU Anthanont, P Polisecki, E Asztalos, BF Diffenderfer, MR Barrett, PHR Millar, JS Billheimer, J Cuchel, M Rader, DJ Schaefer, EJ AF Anthanont, Pimjai Polisecki, Eliana Asztalos, Bela F. Diffenderfer, Margaret R. Barrett, P. Hugh R. Millar, John S. Billheimer, Jeffrey Cuchel, Marina Rader, Daniel J. Schaefer, Ernst J. TI A novel ApoA-I truncation (ApoA-I-Mytilene) associated with decreased ApoA-I production SO ATHEROSCLEROSIS LA English DT Article DE Genetic mutation; Apolipoprotein kinetics; HDL subpopulations; Cellular cholesterol efflux ID APOLIPOPROTEIN-A-I; ESTER TRANSFER PROTEIN; DENSITY-LIPOPROTEIN DEFICIENCY; CORONARY-HEART-DISEASE; LOW HDL-CHOLESTEROL; VIVO METABOLISM; MUTANT APOLIPOPROTEIN; CORNEAL OPACITIES; TERMINAL DOMAIN; LIPID EFFLUX AB Objective: We report a novel apolipoprotein (apo) A-I truncation (apoA-I-Mytilene) due to a heterozygous nonsense mutation (c.718C > T, p. Gln216*) in a 68-year-old male proband with premature coronary heart disease (CHD), corneal arcus, and very low plasma concentrations of HDL cholesterol (HDL-C) and apoA-I. Two family members also had the same mutation. Our objectives were to characterize the kindred and to examine the kinetics of apoA-I, as well as cellular cholesterol efflux capacity in the proband. Methods: We carried out the kinetic studies using a primed constant infusion of [5,5,5-D-3] L-leucine and isotopic enrichment was determined by gas chromatography mass spectrometry in the proband and seven controls with low HDL-C. To assess cellular cholesterol efflux capacity, we used a validated ex vivo system that involved incubation of J774 macrophages with apoB-depleted serum from the proband, five controls with normal HDL-C, and two controls with low HDL-C. Results: Stable isotope kinetic studies indicated that the proband had an apoA-I production rate (PR) that was 41% lower than the mean PR observed in low HDL-C controls (n = 7). The cellular cholesterol efflux capacity assessment showed normalized cholesterol efflux capacity in the proband was decreased by 36% compared to the mean normalized cholesterol efflux capacity of normal controls (n = 5). Conclusions: Our data indicate that this novel heterozygous apoA-I truncation is associated with markedly decreased levels of HDL-C, plasma apoA-I, and apoA-I in large alpha-1 HDL particles, as well as decreased total cellular cholesterol efflux and decreased apoA-I production. (C) 2014 Published by Elsevier Ireland Ltd. C1 [Anthanont, Pimjai; Asztalos, Bela F.; Diffenderfer, Margaret R.; Schaefer, Ernst J.] Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Polisecki, Eliana; Asztalos, Bela F.; Schaefer, Ernst J.] Boston Heart Diagnost, Boston, MA 01702 USA. [Barrett, P. Hugh R.] Univ Western Australia, Sch Med & Pharmacol, Metab Res Ctr, Perth, WA 6009, Australia. [Barrett, P. Hugh R.] Univ Western Australia, Fac Engn Comp & Math, Perth, WA 6009, Australia. [Millar, John S.] Univ Penn, Perelman Sch Med, Div Translat Med & Human Genet, Dept Med, Philadelphia, PA 19104 USA. [Billheimer, Jeffrey; Cuchel, Marina; Rader, Daniel J.] Univ Penn, Perelman Sch Med, Inst Translat Med & Therapeut, Dept Med, Philadelphia, PA 19104 USA. RP Schaefer, EJ (reprint author), Tufts Univ, Cardiovasc Nutr Lab, Jean Mayer USDA Human Nutr Res Ctr Aging, 711 Washington St, Boston, MA 02111 USA. EM ernst.schaefer@tufts.edu RI Barrett, Hugh/B-2745-2011; OI Barrett, Peter Hugh/0000-0003-3223-6125 FU National Institutes of Health [R01-HL-60935, P50 HL083813-01]; Department of Clinical Research, Medicinal Products Research and Development, Pfizer, Inc., Groton, CT; US Department of Agriculture Research Service Contract [53-3K-06] FX This research was supported by National Institutes of Health Grant R01-HL-60935 (E.J.S.), by contracts from the Department of Clinical Research, Medicinal Products Research and Development, Pfizer, Inc., Groton, CT (E.J.S.), and the US Department of Agriculture Research Service Contract 53-3K-06 (E.J.S.), and Project Grant P50 HL083813-01 from the National Institutes of Health (E.J.S.). The contents are solely the responsibility of the authors and do not necessarily represent the official views of Pfizer, the U.S. Department of Agriculture, or the National Institutes of Health. P.A. is a postdoctoral research fellow from Thammasat University, Thailand. NR 43 TC 5 Z9 5 U1 0 U2 2 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD AUG PY 2014 VL 235 IS 2 BP 470 EP 476 DI 10.1016/j.atherosclerosis.2014.05.935 PG 7 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AP9PF UT WOS:000342411000933 PM 24950002 ER PT J AU Corella, D Sorli, JV Asensio, EM Coltell, O Barragan-Arnal, R Ordovas, JM Ortega-Azorin, C AF Corella, D. Sorli, J. V. Asensio, E. M. Coltell, O. Barragan-Arnal, R. Ordovas, J. M. Ortega-Azorin, C. TI THE RS9939609-FTO POLYMORPHISM IS ASSOCIATED WITH METHYLATION LEVELS IN BOTH MEN AND WOMEN FROM A HIGH CARDIOVASCULAR RISK POPULATION SO ATHEROSCLEROSIS LA English DT Meeting Abstract CT 82nd Congress of the European-Atherosclerosis-Society (EAS) CY MAY 31-JUN 03, 2014 CL Spanish Soc Atherosclerosis, Madrid, SPAIN SP European Atherosclerosis Soc HO Spanish Soc Atherosclerosis C1 [Corella, D.; Sorli, J. V.; Asensio, E. M.; Barragan-Arnal, R.; Ortega-Azorin, C.] Univ Valencia, CIBER OBN, Valencia, Spain. [Coltell, O.] Univ Jaume 1, Castellon de La Plana, Spain. [Ordovas, J. M.] Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD AUG PY 2014 VL 235 IS 2 MA EAS-0837 BP E128 EP E129 PG 2 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AP9PF UT WOS:000342411000355 ER PT J AU Corella, D Sorli, JV Coltell, O Ortega-Azorin, C Carrasco, P Quiles, L Gonzalez, JI Guillem-Saiz, P Ordovas, JM AF Corella, D. Sorli, J. V. Coltell, O. Ortega-Azorin, C. Carrasco, P. Quiles, L. Gonzalez, J. I. Guillem-Saiz, P. Ordovas, J. M. TI ADHERENCE TO THE MEDITERRANEAN DIET MODULATES THE ASSOCIATION BETWEEN THE ABCA1 GENE, PLASMA LIPIDS AND DIABETES IN A HIGH-RISK POPULATION SO ATHEROSCLEROSIS LA English DT Meeting Abstract CT 82nd Congress of the European-Atherosclerosis-Society (EAS) CY MAY 31-JUN 03, 2014 CL Spanish Soc Atherosclerosis, Madrid, SPAIN SP European Atherosclerosis Soc HO Spanish Soc Atherosclerosis C1 [Corella, D.; Sorli, J. V.; Ortega-Azorin, C.; Carrasco, P.; Quiles, L.; Gonzalez, J. I.; Guillem-Saiz, P.] Univ Valencia, CIBER OBN, Valencia, Spain. [Coltell, O.] Univ Jaume 1, Castellon de La Plana, Spain. [Ordovas, J. M.] Tufts Univ, USDA, Human Nutr Res Ctr, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 1 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD AUG PY 2014 VL 235 IS 2 MA EAS-0437 BP E115 EP E115 PG 1 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AP9PF UT WOS:000342411000310 ER PT J AU Moreno-Franco, B Leon-Latre, M Andres-Esteban, EM Ordovas, JM Casasnovas, JA Penalvo, JL AF Moreno-Franco, B. Leon-Latre, M. Andres-Esteban, E. M. Ordovas, J. M. Casasnovas, J. A. Penalvo, J. L. TI SOLUBLE AND INSOLUBLE DIETARY FIBRE INTAKE AND RISK FACTORS FOR CVD AND METABOLIC SYNDROME IN MIDDLE-AGED ADULTS: THE AWHS COHORT SO ATHEROSCLEROSIS LA English DT Meeting Abstract CT 82nd Congress of the European-Atherosclerosis-Society (EAS) CY MAY 31-JUN 03, 2014 CL Spanish Soc Atherosclerosis, Madrid, SPAIN SP European Atherosclerosis Soc HO Spanish Soc Atherosclerosis C1 [Moreno-Franco, B.; Leon-Latre, M.; Casasnovas, J. A.] INST ARAGONES CIENCIAS SALUD, Cardiovasc Prevent Unit, Zaragoza, Spain. [Andres-Esteban, E. M.] INST INVEST HOSP 12 OCTUBRE, Clin Res Unit, Madrid, Spain. [Ordovas, J. M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Penalvo, J. L.] CTR NACL INVEST CARDIOVASC, Dept Epidemiol Atherothrombosis & Cardiovasc Imag, Madrid, Spain. NR 0 TC 0 Z9 0 U1 0 U2 4 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD AUG PY 2014 VL 235 IS 2 MA EAS-0095 BP E279 EP E280 PG 3 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AP9PF UT WOS:000342411000831 ER PT J AU Moreno-Franco, B Andres-Esteban, EM Ledesma, M Laclaustra, M Penalvo, JL Pocovi, M Ordovas, JM Casasnovas, JA Leon-Latre, M AF Moreno-Franco, B. Andres-Esteban, E. M. Ledesma, M. Laclaustra, M. Penalvo, J. L. Pocovi, M. Ordovas, J. M. Casasnovas, J. A. Leon-Latre, M. TI STUDY OF SEDENTARY TIME DEFINITIONS AND RISK OF METABOLIC SYNDROME IN A MIDDLE-AGED WORKING POPULATION: THE AWHS COHORT SO ATHEROSCLEROSIS LA English DT Meeting Abstract CT 82nd Congress of the European-Atherosclerosis-Society (EAS) CY MAY 31-JUN 03, 2014 CL Spanish Soc Atherosclerosis, Madrid, SPAIN SP European Atherosclerosis Soc HO Spanish Soc Atherosclerosis C1 [Moreno-Franco, B.; Ledesma, M.; Casasnovas, J. A.; Leon-Latre, M.] Inst Aragones Ciencias Salud, Cardiovasc Prevent Unit, Zaragoza, Spain. [Andres-Esteban, E. M.] Hosp 12 Octubre, Inst Invest, Clin Res Unit, E-28041 Madrid, Spain. [Laclaustra, M.; Penalvo, J. L.] Ctr Nacl Invest Cardiovasc, Dept Epidemiol Atherothrombosis & Cardiovasc Imag, Madrid, Spain. [Pocovi, M.] Univ Zaragoza, Dept Biochem & Mol & Cellular Biol, Zaragoza, Spain. [Ordovas, J. M.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. NR 0 TC 0 Z9 0 U1 0 U2 3 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0021-9150 EI 1879-1484 J9 ATHEROSCLEROSIS JI Atherosclerosis PD AUG PY 2014 VL 235 IS 2 MA EAS-0569 BP E213 EP E214 PG 2 WC Cardiac & Cardiovascular Systems; Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AP9PF UT WOS:000342411000624 ER PT J AU McRoberts, RE Cohen, WB Pflugmacher, D AF McRoberts, Ronald E. Cohen, Warren B. Pflugmacher, Dirk TI Preface, 2012 ForestSAT Special Issue SO REMOTE SENSING OF ENVIRONMENT LA English DT Editorial Material C1 [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Cohen, Warren B.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. [Pflugmacher, Dirk] Humboldt Univ, Dept Geog, D-10099 Berlin, Germany. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, St Paul, MN 55108 USA. NR 16 TC 3 Z9 3 U1 1 U2 10 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 1 EP 2 DI 10.1016/j.rse.2014.02.010 PG 2 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000001 ER PT J AU Ohmann, JL Gregory, MJ Roberts, HM AF Ohmann, Janet L. Gregory, Matthew J. Roberts, Heather M. TI Scale considerations for integrating forest inventory plot data and satellite image data for regional forest mapping SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Vegetation mapping; Nearest-neighbor imputation; Landsat time-series; Spatial resolution; Regional inventory plots; Landscape pattern ID NEAREST-NEIGHBOR IMPUTATION; DIRECT GRADIENT ANALYSIS; THEMATIC MAPPER DATA; LANDSAT TIME-SERIES; REMOTE-SENSING DATA; ACCURACY-ASSESSMENT; COASTAL OREGON; DISTURBANCE; VEGETATION; RESOLUTION AB The integration of satellite image data with forest inventory plot data is a popular approach for mapping forest vegetation over large regions. Several methodological choices regarding spatial scale, mostly related to spatial resolution or grain, can profoundly influence forest maps developed from plot and imagery data. Yet often the consequences of scaling choices are not explicitly addressed. Our objective was to quantify the effects of several scale-related methods on map accuracy for multiple forest attributes, using a variety of diagnostics that address different map characteristics, to help guide map developers and users. We conducted nearest-neighbor imputation over a large region in the Pacific Northwest, USA, to investigate effects of imputation grain (single pixel or kernel); inclusion of heterogeneous plots; accuracy assessment grain and extent; and value of k (k = 1 and k = 5), where k is the number of nearest-neighbor plots. Spatial predictors were from rasters describing climate and topography and a time-series of Landsat imagery. Reference data were from regional forest inventory plots measured over two decades. All analyses were conducted at a spatial resolution of 30 m x 30 m. Effects of imputation grain and heterogeneous plots on map accuracy were small. Excluding heterogeneous plots slightly improved map accuracy and did not lessen the systematic agreement (AC(sys)) between our maps and observed plot data. Accuracy assessment grain strongly influenced map accuracy: maps assessed with a multi-pixel block were much more accurate than when assessed with a single pixel for almost all map diagnostics, but this was an artifact of methods rather than reflecting real differences among maps. Unsystematic agreement (AC(UNS)) between our maps and plots, or random error, improved notably with increasing accuracy assessment extent for all scaling methods, indicating that reliability of most map applications can be improved through coarsening the map grain. Value of k strongly influenced map diagnostics. The k = 5 maps were better than k = 1 maps for local-scale accuracy, but at the cost of reduced AC(sys), and loss of variability and poor areal representation of forest conditions over the study region. The k = 1 maps produced notably better predictions of the least abundant forest conditions (early-successional, late-successional, and broadleaf). None of the scaling methods were optimal for all map diagnostics. Nevertheless, given a variety of diagnostics associated with a range of scaling options, map developers and map users can make informed choices about methods and resulting maps that best meet their particular objectives, and we present some general guidelines in this regard. Most of our findings are applicable to mapping with Landsat data in other forested regions with similar forest inventory data, and to other methods for spatial prediction. Published by Elsevier Inc. C1 [Ohmann, Janet L.] US Forest Serv, Pacific NW Res Stn, USDA, Corvallis, OR 97331 USA. [Gregory, Matthew J.; Roberts, Heather M.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. RP Ohmann, JL (reprint author), US Forest Serv, Pacific NW Res Stn, USDA, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM janet.ohmann@oregonstate.edu FU USDA Forest Service; Pacific Northwest Research Station; Pacific Northwest Region FX We thank Ron McRoberts, Steve Stehman, Harold Zald, Rachel Riemann, and two anonymous reviewers for their helpful comments on earlier versions of this manuscript. This research was supported by the USDA Forest Service, Pacific Northwest Research Station and Pacific Northwest Region. We also thank the regional forest inventory programs for the wealth of invaluable field plot data that make this research possible. NR 45 TC 7 Z9 7 U1 4 U2 27 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 3 EP 15 DI 10.1016/j.rse.2013.08.048 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000002 ER PT J AU Kane, VR North, MP Lutz, JA Churchill, DJ Roberts, SL Smith, DF McGaughey, RJ Kane, JT Brooks, ML AF Kane, Van R. North, Malcolm P. Lutz, James A. Churchill, Derek J. Roberts, Susan L. Smith, Douglas F. McGaughey, Robert J. Kane, Jonathan T. Brooks, Matthew L. TI Assessing fire effects on forest spatial structure using a fusion of Landsat and airborne LiDAR data in Yosemite National Park SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Fire severity; Forest structure; Relativized differenced Normalized Burn Ratio (RdNBR); LiDAR; Gaps; Tree clumps; Sierra Nevada; Abies concolor; Abies magnifica; Pinus lambertiana; Pinus ponderosa ID MIXED-CONIFER FOREST; NORTHERN SIERRA-NEVADA; PONDEROSA PINE FOREST; FUEL REDUCTION; SILVICULTURAL PRESCRIPTIONS; RESTORATION TREATMENTS; REGENERATION PATTERNS; VEGETATION STRUCTURE; SOUTHERN CASCADES; PACIFIC-NORTHWEST AB Mosaics of tree clumps and openings are characteristic of forests dominated by frequent low- and moderate-severity fires. When restoring these fire-suppressed forests, managers often try to reproduce these structures to increase ecosystem resilience. We examined unburned and burned forest structures for 1937 0.81 ha sample areas in Yosemite National Park, USA. We estimated severity for fires from 1984 to 2010 using the Landsat-derived Relativized differenced Normalized Burn Ratio (RdNBR) and measured openings and canopy clumps in five height strata using airborne LiDAR data. Because our study area lacked concurrent field data, we identified methods to allow structural analysis using LiDAR data alone. We found three spatial structures, canopy-gap, clump-open, and open, that differed in spatial arrangement and proportion of canopy and openings. As fire severity increased, the total area in canopy decreased while the number of clumps increased, creating a patchwork of openings and multistory tree clumps. The presence of openings >0.3 ha, an approximate minimum gap size needed to favor shade-intolerant pine regeneration, increased rapidly with loss of canopy area. The range and variation of structures for a given fire severity were specific to each forest type. Low- to moderate-severity fires best replicated the historic clump-opening patterns that were common in forests with frequent fire regimes. Our results suggest that managers consider the following goals for their forest restoration: 1) reduce total canopy cover by breaking up large contiguous areas into variable-sized tree clumps and scattered large individual trees; 2) create a range of opening sizes and shapes, including similar to 50% of the open area in gaps >0.3 ha; 3) create multistory clumps in addition to single story clumps; 4) retain historic densities of large trees; and 5) vary treatments to include canopy-gap, clump-open, and open mosaics across project areas to mimic the range of patterns found for each forest type in our study. (C) 2013 Elsevier Inc. All rights reserved. C1 [Kane, Van R.; Churchill, Derek J.; Kane, Jonathan T.] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [North, Malcolm P.] US Forest Serv, USDA, Pacific Southwest Res Stn, Davis, CA 95618 USA. [Lutz, James A.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Roberts, Susan L.; Smith, Douglas F.] Yosemite Natl Pk, Yosemite, CA 95389 USA. [McGaughey, Robert J.] Univ Washington, Pacific NW Res Stn, US Forest Serv, USDA, Seattle, WA 98195 USA. [Brooks, Matthew L.] US Geol Survey, Western Ecol Res Ctr, Yosemite Field Stn, El Portal, CA 95318 USA. RP Kane, VR (reprint author), Univ Washington, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. EM vkane@uw.edu FU National Park Service, Fire and Aviation Management Branch, Fuels and Ecology Program [F8803100015]; U.S. Geological Survey Terrestrial, Freshwater, and Marine Environments Program FX M. Meyer (USDA Forest Service) and two anonymous reviewers provided valuable comments that improved the paper. We thank Yosemite National Park for data and assistance with field logistics. Funding was provided by the National Park Service, Fire and Aviation Management Branch, Fuels and Ecology Program (Interagency Agreement F8803100015), and the U.S. Geological Survey Terrestrial, Freshwater, and Marine Environments Program. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the US government. NR 86 TC 12 Z9 12 U1 5 U2 59 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 89 EP 101 DI 10.1016/j.rse.2013.07.041 PG 13 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000008 ER PT J AU Krofcheck, DJ Eitel, JUH Vierling, LA Schulthess, U Hilton, TM Dettweiler-Robinson, E Pendleton, R Litvak, ME AF Krofcheck, Dan J. Eitel, Jan U. H. Vierling, Lee A. Schulthess, Urs Hilton, Timothy M. Dettweiler-Robinson, Eva Pendleton, Rosemary Litvak, Marcy E. TI Detecting mortality induced structural and functional changes in a pinon-juniper woodland using Landsat and Rapid Eye time series SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Semi-arid; High resolution; Time-series; Ecosystem disturbance; Eddy-covariance; Red-edge ID SPECTRAL MIXTURE ANALYSIS; NET PRIMARY PRODUCTION; DIFFERENTIAL RESPONSES; FOREST DISTURBANCE; VEGETATION INDEX; TREE MORTALITY; CARBON BALANCE; SOIL-MOISTURE; UNITED-STATES; ARID REGIONS AB Pinon juniper (PJ) woodlands have recently undergone dramatic drought-induced mortality, triggering broad scale structural changes in this extensive Southwestern US biome. Given that climate projections for the region suggest widespread conifer mortality is likely to continue into the next century, it is critical to better understand how this climate-induced change in vegetation structure alters ecosystem function (e.g., productivity, biogeochemical cycling, energy partitioning) in PJ woodlands. Data from satellite remote sensing could potentially help to gain some of this understanding. However, relatively little is known about the suitability of satellite remote sensing for monitoring mortality induced structural and functional changes in PJ woodlands, a semi-arid biome characterized by sparse vegetation that complicates remote sensing of vegetation properties. Here we examined the potential role of satellite remote sensing to better understand structural and functional changes that take place in PJ woodlands as they respond to and recover from climate induced mortality events. We used time series medium (30 x 30 m) and high (<= 5 x 5 m) spatial resolution satellite data in concert with eddy covariance-based gross primary productivity (GPP) data collected in a PJ woodland mortality manipulation near Mountainair, NM. Our results showed that both high and medium resolution satellite remote sensing data can provide ecosystem level assessments of overall canopy mortality and subsequent regrowth in these semiarid woodlands. However, high spatial resolution data allowed detecting the disturbance almost a full year earlier and enabled us to describe the spatially heterogeneous patterns of recovery typical for such complex. The ability of satellite remote sensing to be used to provide information about changes in ecosystem function was conditional on the presence of sufficient soil moisture; when soil moisture was present the strength of the linear relationship between GPP and spectral vegetation indices improved at the manipulated site (e.g. for high resolution NDVI data R-2 = 0.13 during drought conditions, R-2 = 0.46 when wet, P < .05) and less so at a nearby control site (R-2 = 0.09 during drought conditions, compared to R-2 = 0.22 during growing conditions, P < .05). Thus, although satellite time series can be used to monitor and improve our understanding of canopy function in both disturbed and undisturbed semi-arid coniferous biomes, combining in situ eddy covariance data with satellite time series demonstrated that the utility of these datasets is highest during periods of peak growing conditions. This study therefore provides a benchmark for understanding how spatial and temporal variation in vegetation structure and function can be best studied as semi-arid coniferous biomes respond to future climate variability. (C) 2013 Elsevier Inc. All rights reserved. C1 [Krofcheck, Dan J.; Hilton, Timothy M.; Dettweiler-Robinson, Eva; Litvak, Marcy E.] Univ New Mexico, Dept Biol, Albuquerque, NM 87131 USA. [Eitel, Jan U. H.; Vierling, Lee A.] Univ Idaho, Geospatial Lab Environm Dynam, Moscow, ID 83844 USA. [Eitel, Jan U. H.; Vierling, Lee A.] Univ Idaho, McCall Outdoor Sci Sch, Mccall, ID 83638 USA. [Schulthess, Urs] 4DMaps, D-10405 Berlin, Germany. [Pendleton, Rosemary] US Forest Serv, USDA, Rocky Mt Res Stn, Albuquerque, NM 87102 USA. RP Krofcheck, DJ (reprint author), Univ New Mexico, Dept Biol, 1 Univ Blvd Northeast, Albuquerque, NM 87131 USA. EM djkrofch@unm.edu; jeitel@uidaho.edu; leev@uidaho.edu; uschulthess@gmail.com; evadr@unm.edu; rpendleton@fs.fed.us; mlitvak@unm.edu RI Vierling, Lee/E-6428-2010 OI Vierling, Lee/0000-0001-5344-1983 FU Office of Science (BER); United States Geological Survey (USGS) [08HQGRO157]; AmericaView; National Science Foundation; NM EPSCoR; USFS; New Mexico Space Grant Consortium (NMSGC); US Department of Energy FX This research was supported primarily by the Office of Science (BER), US Department of Energy. We also acknowledge the funding from grant/cooperative agreement number 08HQGRO157 from the United States Geological Survey (USGS) via a subaward from AmericaView, the National Science Foundation through funds to the Sevilleta LTER site and NM EPSCoR, in addition to the funding from the USFS for support of the flux towers, and from the New Mexico Space Grant Consortium (NMSGC) for the partial funding of satellite data acquisitions. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the Office of Science, US Department of Energy, NSF, or USGS. Many thanks to Andy Fox, Leo Stoscheck and Skyler Hackley for the intellectual and physical support of this project, and to Nate McDowell, Thom Rahn and Bob Sinsabaugh for their help in establishing the treatments. We also thank RapidEye for making a portion of the dense time series freely available for this study. Finally we thank the three anonymous reviewers and David Turner for their comments and revisions that greatly improved the quality of the manuscript. NR 60 TC 8 Z9 8 U1 3 U2 32 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 102 EP 113 DI 10.1016/j.rse.2013.11.009 PG 12 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000009 ER PT J AU Pflugmacher, D Cohen, WB Kennedy, RE Yang, ZQ AF Pflugmacher, Dirk Cohen, Warren B. Kennedy, Robert E. Yang, Zhiqiang TI Using Landsat-derived disturbance and recovery history and lidar to map forest biomass dynamics SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Landsat; Time series; Forest disturbance; Biomass; Carbon; Lidar; Tasseled cap; LandTrendr ID MULTITEMPORAL SATELLITE IMAGERY; DOUGLAS-FIR FORESTS; ABOVEGROUND BIOMASS; WESTERN OREGON; TIME-SERIES; CARBON STORAGE; STAND STRUCTURE; BOREAL FORESTS; PONDEROSA PINE; YOUNG FORESTS AB Improved monitoring of forest biomass and biomass change is needed to quantify natural and anthropogenic effects on the terrestrial carbon cycle. Landsat's temporal and spatial coverage, moderate spatial resolution, and long history of earth observations provide a unique opportunity for characterizing vegetation changes across large areas and long time scales. However, like with other multi-spectral passive optical sensors, Landsat's relationship of single-date reflectance with forest biomass diminishes under high leaf area and complex canopy conditions. Because the condition of a forest stand at any point in time is largely determined by its disturbance and recovery history, we conceived a method that enhances Landsat's spectral relationships with biomass by including information on vegetation trends prior to the date for which estimates are desired. With recently developed algorithms that characterize trends in disturbance (e.g. year of onset, duration, and magnitude) and post-disturbance regrowth, it should now be possible to realize improved Landsat-based mapping of current biomass across large regions. Moreover, given that we now have 40 years of Landsat data, it should also be possible to use this approach to map historic biomass densities. In this study, we developed regression tree models to predict current forest aboveground biomass (AGB) for a mixed-conifer region in eastern Oregon (USA) using Landsat-based disturbance and recovery (DR) metrics. We employed the trajectory-fitting algorithm LandTrendr to characterize DR trends from yearly Landsat time series between 1972 and 2010. The most important DR predictors of AGB were associated with magnitude of disturbance, post-disturbance condition and post-disturbance recovery, whereas time since disturbance and pre-disturbance trends showed only weak correlations with AGB. Including DR metrics substantially improved predictions of AGB (RMSE = 30.3 Mg ha(-1), 27%) compared to models based on only single-date reflectance (RMSE = 39.6 Mg ha(-1), 35%). To determine the number of years required to adequately capture the effect of DR on AGB, we explored the relationship between time-series length and model prediction accuracy. Prediction accuracy increased exponentially with increasing number of years across the entire observation period, suggesting that in this forest region the longer the historic record of disturbance and recovery metrics the more accurate the mapping of AGB. However, time series lengths of between 10 and 20 years were adequate to significantly improve model predictions, and lengths of as little as 5 years still had a meaningful impact To test the concept of historic biomass prediction, we applied our model to Landsat time series from 1972-1993 and estimated AGB biomass change between 1993 and 2007. Our estimates compared well with historic inventory data, demonstrating that long-term Landsat observations of DR processes can aid in monitoring AGB and AGB change. Instead of directly linking Landsat data with the limited amount of available field-based AGB data, in this study we used the field data to map AGB with airborne lidar and then sampled the lidar data for model training and error assessment By using lidar data to build and test our prediction model, this study illustrates that lidar data have great value for scaling between field measurements and Landsat data. (C) 2013 Elsevier Inc All rights reserved. C1 [Pflugmacher, Dirk; Yang, Zhiqiang] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Cohen, Warren B.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. [Kennedy, Robert E.] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. RP Pflugmacher, D (reprint author), Humboldt Univ, Dept Geog, Unter Linden 6, D-10099 Berlin, Germany. EM dirk.pflugmacher@geo.hu-berlin.de FU NASA Headquarters under the NASA Earth and Space Science Fellowship Program [NNX10AN49H]; Oregon Watershed Enhancement Board FX This work was supported by NASA Headquarters under the NASA Earth and Space Science Fellowship Program - Grant "NNX10AN49H" and by the Oregon Watershed Enhancement Board. The authors would like to thank Janet Ohmann and Heather Roberts for their valuable help with the CVS data. NR 86 TC 21 Z9 24 U1 15 U2 62 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 124 EP 137 DI 10.1016/j.rse.2013.05.033 PG 14 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000011 ER PT J AU Sannier, C McRoberts, RE Fichet, LV Makaga, EMK AF Sannier, Christophe McRoberts, Ronald E. Fichet, Louis-Vincent Makaga, Etienne Massard K. TI Using the regression estimator with Landsat data to estimate proportion forest cover and net proportion deforestation in Gabon SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE REDD; Two-stage estimation ID IMAGE-BASED MAPS; ACCURACY ASSESSMENT; TROPICAL FORESTS; AREA ESTIMATION; INFERENCE; CLOUD AB Forest cover maps were produced for the Gabonese Agency for Space Studies and Observations (AGEOS) for 1990, 2000 and 2010 for an area of approximately 102,000 km(2) corresponding to 38% of the total area of Gabon and representative of the range of human pressure on forest resources. The maps were constructed using a combination of a semi-automated classification procedure and manual enhancements to ensure the greatest possible accuracy. A two-stage area frame sampling approach was adopted to collect reference data for assessing the accuracy of the forest cover maps and to estimate proportion forest cover and net proportion deforestation. A total of 251 2 x 2 km segments or primary sample units (PSUs) were visually interpreted by a team of photo-interpreters independently from the map production team to produce a reference dataset representing about 1% of the study area. Paired observations were extracted from the forest cover map and the reference data for a random selection of 50 secondary sample units (SSUs) in the form of pixels within each PSU. Overall map accuracies were greater than 95%. PSU and SSU outputs were used to estimate proportion forest cover and net proportion deforestation using both direct expansion and model-assisted regression (MAR) estimators. All proportion forest cover estimates were similar, but the variances of the MAR estimates were smaller than variances for the direct expansion estimates by factors as great as 50. In addition, SSU-level estimates had standard errors slightly greater than those of PSU-level estimates, but the differences were small, particularly when auxiliary variables were obtained from forest cover maps. Therefore, a two-stage sampling approach was justified for collecting a reliable forest cover reference dataset for estimating proportion forest cover area and net proportion deforestation. Finally, despite large overall map accuracies, net proportion deforestation estimates obtained from the maps alone can be misleading as indicated by the finding that the MAR estimates, which included adjustment for bias estimates, were twice the non-adjusted map estimates for the periods 1990-2000 and 1990-2010. The results confirmed the expected generally small level of net deforestation for Gabon. However, loss of forest cover appears to have almost stopped in the last 10 years. One explanation could be the creation of national parks and the implementation of forest concession management plans from 2000 onward, but this should be further explored. (C) 2013 Elsevier Inc. All rights reserved. C1 [Sannier, Christophe; Fichet, Louis-Vincent] SIRS, F-59650 Villeneuve Dascq, France. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN USA. [Makaga, Etienne Massard K.] AGEOS, Libreville, Gabon. RP Sannier, C (reprint author), SIRS, Parc Cimaise,27 Rue Carrousel, F-59650 Villeneuve Dascq, France. EM christophe.sannier@sirs-fr.com FU European Space Agency (ESA); EU FX The production of forest cover and forest cover change maps was funded under the European Space Agency (ESA) Global Monitoring for the Environment and Security Service Element on Forest Monitoring REDD extension project (GSE FM REDD). Methodological developments related to forest cover area and forest cover change area estimates were funded under the EU FP7 GMES REDDAF project. Both projects were coordinated by GAF AG. The authors thank Professor Timothy G. Gregoire, Yale University, for consultations on and references for the model-assisted, regression estimator. NR 41 TC 12 Z9 14 U1 0 U2 19 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 138 EP 148 DI 10.1016/j.rse.2013.09.015 PG 11 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000012 ER PT J AU McRoberts, RE AF McRoberts, Ronald E. TI Post-classification approaches to estimating change in forest area using remotely sensed auxiliary data SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Model-assisted regression estimator; Model-based estimator; Bootstrap ID MODEL-BASED INFERENCE; LIDAR SAMPLE SURVEY; IMAGE-BASED MAPS; ASSISTED ESTIMATION; HEDMARK COUNTY; BIOMASS ESTIMATION; TROPICAL FORESTS; AIRBORNE LIDAR; NORWAY; DEFORESTATION AB Multiple remote sensing-based approaches to estimating gross afforestation, gross deforestation, and net deforestation are possible. However, many of these approaches have severe data requirements in the form of long time series of remotely sensed data and/or large numbers of observations of land cover change to train classifiers and assess the accuracy of classifications. In particular, when rates of change are small and equal probability sampling is used, observations of change may be scarce. For these situations, post-classification approaches may be the only viable alternative. The study focused on model-assisted and model-based approaches to inference for post-classification estimation of gross afforestation, gross deforestation, and net deforestation using Landsat imagery as auxiliary data. Emphasis was placed on estimation of variances to support construction of statistical confidence intervals for estimates. Both analytical and bootstrap approaches to variance estimation were used. For a study area in Minnesota, USA, estimates of net deforestation were not statistically significantly different from zero. Published by Elsevier Inc. C1 US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, St Paul, MN 55108 USA. NR 41 TC 8 Z9 8 U1 1 U2 18 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 149 EP 156 DI 10.1016/j.rse.2013.03.036 PG 8 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000013 ER PT J AU Andersen, HE Reutebuch, SE McGaughey, RJ d'Oliveira, MVN Keller, M AF Andersen, Hans-Erik Reutebuch, Stephen E. McGaughey, Robert J. d'Oliveira, Marcus V. N. Keller, Michael TI Monitoring selective logging in western Amazonia with repeat lidar flights SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Forest biomass; Airborne laser scanning; Selective logging; Tropical forest management; Lidar; Amazon forest monitoring ID PULSE REPETITION FREQUENCIES; ESTIMATING FOREST BIOMASS; AIRBORNE LASER; BRAZILIAN AMAZON; FLYING ALTITUDE; CARBON; EMISSIONS; DEFORESTATION; INTENSITY; DYNAMICS AB The objective of this study was to test the use of repeat flight, airborne laser scanning data (lidar) for estimating changes associated with low-impact selective logging (approx. 10-15 m(3) ha(-1) = 5-7% of total standing volume harvested) in natural tropical forests in the Western Brazilian Amazon. Specifically, we investigated change in area impacted by selective logging, change in tall canopy (30 m +) area, change in lidar canopy structure metrics, and change in above ground biomass (AGB) using a model-based statistical framework. Ground plot measurements were only available from the time of the 2010 lidar acquisition. A simple differencing of the 2010 and 2011 lidar canopy height models identified areas where canopy over 30 m tall had been removed. Area of tall canopy dropped from 22.8% in 2010 to 18.7% in 2011, a reduction of 4.1%. Using a relative density model (RDM) technique the increase in area of roads, skidtrails, landings, and felled tree gaps was estimated to be 17.1%. A lidar-based regression model for estimating AGB was developed using lidar metrics from the 2010 lidar acquisition and corresponding AGB ground plot measurements. The estimator was then used to compute AGB estimates for the site in 2010 and 2011 using the 2010 and 2011 lidar acquisition data, respectively. A model-based statistical approach was then used to estimate the uncertainty of the changes in AGB between the acquisitions. Change in RDMs between lidar acquisitions was used to classify each 50 m cell in the study area as impacted or non-impacted by logging. The change in mean AGB for the entire study area was -9.1 Mg ha(-1) +/- 1.9 (mean +/- SD) (P-value < 0.0001). The change in mean AGB for areas newly impacted in 2011 was -17.9 +/- 3.1 Mg ha(-1) (P-value < 0.0001) while the change in mean AGB for non-impacted areas was significantly less at -2.6 +/- 1.1 Mg ha(-1) (P-value = 0.009). These results provide corroborating evidence of the spatial extent and magnitude of change due to low-intensity logging in tropical forests with heavy residual canopy cover. Published by Elsevier Inc. C1 [Andersen, Hans-Erik; Reutebuch, Stephen E.; McGaughey, Robert J.] Univ Washington, Pacific NW Res Stn, US Forest Serv, USDA, Seattle, WA 98195 USA. [d'Oliveira, Marcus V. N.] EMBRAPA CPAF ACRE, BR-69900180 Rio Branco, Brazil. [Keller, Michael] US Forest Serv, USDA, Int Inst Trop Forestry, San Juan, PR USA. [Keller, Michael] EMBRAPA CNPM, Sao Paulo, Brazil. RP Reutebuch, SE (reprint author), Univ Washington, Pacific NW Res Stn, US Forest Serv, USDA, POB 352100, Seattle, WA 98195 USA. EM sreutebuch@fs.fed.us RI d'Oliveira, Marcus Vinicio Neves/A-2038-2015; Keller, Michael/A-8976-2012 OI Keller, Michael/0000-0002-0253-3359 FU USDA Forest Service International Programs; USAID Sustainable Landscapes FX Lidar data were acquired with support from the USDA Forest Service International Programs and USAID Sustainable Landscapes. NR 54 TC 14 Z9 15 U1 4 U2 23 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 157 EP 165 DI 10.1016/j.rse.2013.08.049 PG 9 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000014 ER PT J AU Skowronski, NS Clark, KL Gallagher, M Birdsey, RA Hom, JL AF Skowronski, Nicholas S. Clark, Kenneth L. Gallagher, Michael Birdsey, Richard A. Hom, John L. TI Airborne laser scanner-assisted estimation of aboveground biomass change in a temperate oak-pine forest SO REMOTE SENSING OF ENVIRONMENT LA English DT Article DE Airborne laser scanning; ALS; Disturbance; Multi-temporal; Biomass change estimation; New Jersey Pinelands ID SIMULATION APPROACH; INVASIVE INSECTS; NATIONAL FOREST; CARBON STOCKS; NEW-JERSEY; LIDAR DATA; GROWTH; UNCERTAINTY; INFORMATION; PINELANDS AB We estimated aboveground tree biomass and change in aboveground tree biomass using repeated airborne laser scanner (ALS) acquisitions and temporally coincident ground observations of forest biomass, for a relatively undisturbed period (2004-2007; Delta(07-04)), a contrasting period of disturbance (2007-2009; Delta(09-07)), and an integrated period (2004-2009; Delta(09-04)). A simple random sampling (SRS) estimator was used to estimate means and variances of biomass and biomass change for each measurement occasion and interval. For each year, linear regression models were used to predict mean total aboveground tree biomass for live, dead, and total biomass components from ALS-derived variables. These models predicted biomass with R-2 = 0.68, 0.59, and 0.70 and RMSEs of 32.7, 30.5, and 31.7 Mg ha(-1) for 2004, 2007 and 2009, respectively. A model assisted indirect estimator was then used to estimate biomass and biomass change for comparison to the field-based SRS estimator. This model assisted indirect approach decreased standard errors of biomass estimation relative to the SRS estimator, but had larger variances for biomass change estimation. Linear regression models were also used to directly predict field-estimated biomass change using ALS A-variables, calculated as the difference between multi-temporal ALS variables, for the study area. Integrated over the 6 year period, these change models had R-2 = 0.81, 0.72, and 0.68 with RMSEs of 2.0, 93, and 1.0 Mg ha(-1) yr(-1) for live, dead, and total aboveground tree biomass, respectively. A model assisted direct estimator reduced standard errors of change estimates by 100-200% compared to the field-based estimates. We discuss several potential advantages and limitations of the direct and indirect approaches. Our primary finding is that model assisted direct estimation of biomass change decreased estimation uncertainty relative to both field and model assisted indirect estimation. Published by Elsevier Inc. C1 [Skowronski, Nicholas S.] US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. [Clark, Kenneth L.; Gallagher, Michael] US Forest Serv, USDA, No Res Stn, New Lisbon, NJ 08064 USA. [Birdsey, Richard A.; Hom, John L.] US Forest Serv, USDA, No Res Stn, Newtown Sq, PA 19073 USA. RP Skowronski, NS (reprint author), 180 Canfield St, Morgantown, WV 26505 USA. EM nskowronski@fs.fed.us FU NASA [NNH08AH971] FX This project was funded in part via NASA grant # NNH08AH971 and the National Fire Plan. The authors would like to thank Jason Cole for his help with extracting biometric data from the Tier 3 database. We also thank Matthew Patterson for his work in collecting much of the field data used here. Finally, we thank Ron McRoberts and two anonymous reviewers for their thoughtful comments and suggestions. NR 57 TC 10 Z9 11 U1 1 U2 16 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0034-4257 EI 1879-0704 J9 REMOTE SENS ENVIRON JI Remote Sens. Environ. PD AUG PY 2014 VL 151 SI SI BP 166 EP 174 DI 10.1016/j.rse.2013.12.015 PG 9 WC Environmental Sciences; Remote Sensing; Imaging Science & Photographic Technology SC Environmental Sciences & Ecology; Remote Sensing; Imaging Science & Photographic Technology GA AP7NM UT WOS:000342264000015 ER PT J AU Arismendi, I Safeeq, M Dunham, JB Johnson, SL AF Arismendi, Ivan Safeeq, Mohammad Dunham, Jason B. Johnson, Sherri L. TI Can air temperature be used to project influences of climate change on stream temperature? SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE air temperature; stream temperature; climate projection; climate change; temperature biases; Mohseni model; salmon ID CONTERMINOUS UNITED-STATES; WATER TEMPERATURE; RIVER TEMPERATURE; PACIFIC-NORTHWEST; TRENDS; MODEL; HABITAT; DISTRIBUTIONS; MICROCLIMATE; DYNAMICS AB Worldwide, lack of data on stream temperature has motivated the use of regression-based statistical models to predict stream temperatures based on more widely available data on air temperatures. Such models have been widely applied to project responses of stream temperatures under climate change, but the performance of these models has not been fully evaluated. To address this knowledge gap, we examined the performance of two widely used linear and nonlinear regression models that predict stream temperatures based on air temperatures. We evaluated model performance and temporal stability of model parameters in a suite of regulated and unregulated streams with 11-44 years of stream temperature data. Although such models may have validity when predicting stream temperatures within the span of time that corresponds to the data used to develop them, model predictions did not transfer well to other time periods. Validation of model predictions of most recent stream temperatures, based on air temperature-stream temperature relationships from previous time periods often showed poor performance when compared with observed stream temperatures. Overall, model predictions were less robust in regulated streams and they frequently failed in detecting the coldest and warmest temperatures within all sites. In many cases, the magnitude of errors in these predictions falls within a range that equals or exceeds the magnitude of future projections of climate-related changes in stream temperatures reported for the region we studied (between 0.5 and 3.0 degrees C by 2080). The limited ability of regression-based statistical models to accurately project stream temperatures over time likely stems from the fact that underlying processes at play, namely the heat budgets of air and water, are distinctive in each medium and vary among localities and through time. C1 [Arismendi, Ivan] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Safeeq, Mohammad] Oregon State Univ, Coll Earth Ocean & Atmospher Sci, Corvallis, OR 97331 USA. [Dunham, Jason B.] US Geol Survey, Forest & Rangeland Ecosyst Sci Ctr, Corvallis, OR 97331 USA. [Johnson, Sherri L.] US Forest Serv, Pacific NW Res Stn, Corvallis, OR 97331 USA. RP Arismendi, I (reprint author), Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. EM Ivan.Arismendi@oregonstate.edu RI Arismendi, Ivan/B-3144-2010 OI Arismendi, Ivan/0000-0002-8774-9350 FU National Science Foundation's Long-Term Ecological Research Program [DEB 08-23380]; US Forest Service Pacific Northwest Research Station; Oregon State University [10-JV-11261991055]; US Geological Survey FX Brooke Penaluna and two anonymous reviewers provided comments that improved the manuscript. Part of the data was provided by the HJ Andrews Experimental Forest research program, funded by the National Science Foundation's Long-Term Ecological Research Program (DEB 08-23380), US Forest Service Pacific Northwest Research Station, and Oregon State University. Financial support for IA was partially provided by US Geological Survey, the US Forest Service Pacific Northwest Research Station and Oregon State University through joint venture agreement 10-JV-11261991055. Use of firm or trade names is for reader information only and does not imply endorsement of any product or service by the US Government. NR 66 TC 14 Z9 14 U1 1 U2 23 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD AUG PY 2014 VL 9 IS 8 AR 084015 DI 10.1088/1748-9326/9/8/084015 PG 12 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AP3HO UT WOS:000341966400015 ER PT J AU Matyas, C Sun, G AF Matyas, Csaba Sun, Ge TI Forests in a water limited world under climate change SO ENVIRONMENTAL RESEARCH LETTERS LA English DT Article DE drylands; ecohydrology; climate forcing; land use change; forest policy; climate forcing; xeric limits ID LOESS PLATEAU; EUROPEAN FOREST; CHINA; IMPACTS; YIELD; VEGETATION; CARBON; STREAMFLOW; REDUCTION; FEEDBACKS AB The debate on ecological and climatic benefits of planted forests at the sensitive dry edge of the closed forest belt (i.e. at the 'xeric limits') is still unresolved. Forests sequester atmospheric carbon dioxide, accumulate biomass, control water erosion and dust storms, reduce river sedimentation, and mitigate small floods. However, planting trees in areas previously dominated by grassland or cropland can dramatically alter the energy and water balances at multiple scales. The forest/grassland transition zone is especially vulnerable to projected drastic temperature and precipitation shifts and growing extremes due to its high ecohydrological sensitivity. We investigated some of the relevant aspects of the ecological and climatic role of forests and potential impacts of climate change at the dryland margins of the temperate-continental zone using case studies from China, the United States and SE Europe (Hungary). We found that, contrary to popular expectations, the effects of forest cover on regional climate might be limited and the influence of forestation on water resources might be negative. Planted forests generally reduce stream flow and lower groundwater table level because of higher water use than previous land cover types. Increased evaporation potential due to global warming and/or extreme drought events is likely to reduce areas that are appropriate for tree growth and forest establishment. Ecologically conscious forest management and forestation planning should be adjusted to the local, projected hydrologic and climatic conditions, and should also consider non-forest alternative land uses. C1 [Matyas, Csaba] Univ West Hungary, Inst Environm & Earth Sci, NEESPI Focus Res Ctr Nonboreal Eastern Europe, H-9401 Sopron, Hungary. [Sun, Ge] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, USDA, Raleigh, NC 27606 USA. RP Matyas, C (reprint author), Univ West Hungary, Inst Environm & Earth Sci, NEESPI Focus Res Ctr Nonboreal Eastern Europe, H-9401 Sopron, Hungary. EM cm@emk.nyme.hu FU EC-FP7 projects 'EVOLTREE' and 'FORGER'; EU-Hungary convergence projects [TAMOP-4.2.2-08/1-2008-0020, TAMOP-4.2.2.A-11/1/Konv]; USDA Forest Service Eastern Forest Environmental Threat Assessment Center FX Research was supported by the EC-FP7 projects 'EVOLTREE' and 'FORGER', as well as by the joint EU-Hungary convergence projects TAMOP-4.2.2-08/1-2008-0020 and TAMOP-4.2.2.A-11/1/Konv. 'Agrarklima'. Partial support was provided by the USDA Forest Service Eastern Forest Environmental Threat Assessment Center. The authors thankfully acknowledge the valuable comments of three anonymous reviewers, essentially contributing to the improvement of the manuscript. NR 60 TC 7 Z9 7 U1 18 U2 89 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 1748-9326 J9 ENVIRON RES LETT JI Environ. Res. Lett. PD AUG PY 2014 VL 9 IS 8 AR 085001 DI 10.1088/1748-9326/9/8/085001 PG 10 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AP3HO UT WOS:000341966400021 ER PT J AU Cammalleri, C Agnese, C Alfieri, JG Drago, A Georgiadis, T Motisi, A Sciortino, M de Bruin, HAR AF Cammalleri, Carmelo Agnese, Carmelo Alfieri, Joseph G. Drago, Antonino Georgiadis, Teodoro Motisi, Antonio Sciortino, Maurizio de Bruin, Henk A. R. TI Exploring the use of displaced-beam scintillometer for daytime measurement of surface energy fluxes over a Mediterranean Olive Orchard SO ITALIAN JOURNAL OF AGROMETEOROLOGY-RIVISTA ITALIANA DI AGROMETEOROLOGIA LA English DT Article DE evapotranspiration; micrometeorological measurements; sparse tall crops; Olea europaea ID SENSIBLE HEAT-FLUX; EDDY COVARIANCE MEASUREMENTS; WATER-VAPOR; APERTURE SCINTILLOMETER; PROFILE RELATIONSHIPS; LAYER SCINTILLOMETRY; STRUCTURE PARAMETER; ROUGHNESS SUBLAYER; BALANCE ALGORITHM; REFRACTIVE-INDEX AB Studies have shown that the footprint of a single eddy covariance (EC) system may not yield representative measurements of the turbulent fluxes at the field scale for sparse vegetated surfaces, whereas scintillometry, due to its larger footprint, may be more suitable for this purpose. However, the latter approach strongly relies on the Monin-Obukhov similarity theory (MOST) that strictly applies in the inertial sub-layer only. This work aims at experimentally confirm the reliability of displaced-beam laser scintillometer (DBLS) measurements over an olive orchard against two EC systems during summer and autumn months of 2007 through 2009. It was found that the DBLS underestimated both the momentum and sensible heat fluxes by 15 to 20% when established retrieval procedures were applied. A new method to determine the sensible heat flux from the DBLS based on the addition of a single-height wind speed measurement was tested, yielding estimates that compare well with the EC observations, with discrepancies in sensible heat fluxes of about 30 to 40 W m(-2). C1 [Cammalleri, Carmelo] Univ Palermo, Dept Civil Environm Aerosp Mat Engn DICAM, Palermo, Italy. [Agnese, Carmelo; Motisi, Antonio] Univ Palermo, Dept Agr & Forest Sci SAF, Palermo, Italy. [Alfieri, Joseph G.] USDA ARS, Hydrol & Remote Sensing Lab, Beltsville, MD 20705 USA. [Drago, Antonino] Assessorato Risorse Agr & Alimentari, Palermo, Italy. [Georgiadis, Teodoro] CNR, Ist Biometeorol IBIMET, I-40126 Bologna, Italy. [Sciortino, Maurizio] CR Casaccia, Agenzia Nuove Tecnol Energia & Svilupo Econ Soste, Rome, Italy. [de Bruin, Henk A. R.] Wageningen Univ, Bilthoven, Netherlands. RP Cammalleri, C (reprint author), Univ Palermo, Dept Civil Environm Aerosp Mat Engn DICAM, Palermo, Italy. EM cammillino@gmail.com FU AGROCLIMA-SICILIA project of the Assessorato Agricoltura e Foreste della Regione Siciliana; Universita degli Studi di Palermo; MIUR FX The authors thank the Ente per le Nuove Tecnologie, l'Energia e l'Ambiente (ENEA) of Palermo, with in particular F. Monteleone and F. Anello, for the helpful collaboration in the scintillometer data acquisition, instrument installation and management, the SIAS (Servizio Informativo Agrometeorologico Siciliano) of the Assessorato Agricoltura e Foreste della Regione Siciliana for supporting both the scintillometer and the eddy covariance installations, and the "Azienda Agricola Rocchetta di Angela Consiglio" for kindly hosting the experiment. This work has been partially funded by the AGROCLIMA-SICILIA project of the Assessorato Agricoltura e Foreste della Regione Siciliana and by PRIN2007-Agnese, co-funded by Universita degli Studi di Palermo and MIUR. NR 58 TC 0 Z9 0 U1 0 U2 1 PU PATRON EDITORE S R L PI BOLOGNA PA VIA BADINI 12, QUARTO INFERIORE, BOLOGNA, 00000, ITALY SN 2038-5625 J9 ITAL J AGROMETEOROL JI Ital. J. Agrometeorol.-Riv. Ital. Agrometeorol. PD AUG PY 2014 VL 19 IS 2 BP 13 EP 28 PG 16 WC Agronomy; Environmental Sciences; Meteorology & Atmospheric Sciences SC Agriculture; Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AP3GQ UT WOS:000341964000002 ER PT J AU Mirando, MA Stormshak, F AF Mirando, M. A. Stormshak, F. TI REPRODUCTION SYMPOSIUM: External influences on reproductive neuroendocrinology SO JOURNAL OF ANIMAL SCIENCE LA English DT Editorial Material C1 [Mirando, M. A.] USDA, Natl Inst Food & Agr, Inst Food Prod & Sustainabil, Div Anim Syst, Washington, DC 20250 USA. [Stormshak, F.] Oregon State Univ, Dept Anim & Rangeland Sci, Corvallis, OR 97331 USA. [Stormshak, F.] Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA. RP Mirando, MA (reprint author), USDA, Natl Inst Food & Agr, Inst Food Prod & Sustainabil, Div Anim Syst, Washington, DC 20250 USA. EM mmirando@nifa.usda.gov NR 5 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2014 VL 92 IS 8 BP 3183 EP 3184 DI 10.2527/jas2014-8219 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA AO9GH UT WOS:000341663600001 PM 25006064 ER PT J AU Htoo, JK Zhu, CL Huber, L de Lange, CFM Quant, AD Kerr, BJ Cromwell, GL Lindemann, MD AF Htoo, J. K. Zhu, C. L. Huber, L. de lange, C. F. M. Quant, A. D. Kerr, B. J. Cromwell, G. L. Lindemann, M. D. TI Determining the optimal isoleucine: lysine ratio for ten- to twenty-two-kilogram and twenty-four- to thirty-nine-kilogram pigs fed diets containing nonexcess levels of leucine SO JOURNAL OF ANIMAL SCIENCE LA English DT Article DE isoleucine; leucine; lysine; performance; pigs; ratio ID PROTEIN-SOURCE; GROWING-PIGS; KG PIGS; SUPPLEMENTATION; REQUIREMENTS; PERFORMANCE; NITROGEN; UREA AB Three 21-d experiments were conducted to determine the optimum standardized ileal digestible (SID) Ile:Lys ratio in 10- to 22-kg and 24- to 39-kg pigs. In Exp. 1, 144 Yorkshire pigs (initial BW = 10.2 kg) were assigned to 6 diets with 6 pens per treatment. Diets 1 to 5 were formulated to contain 5 graded SID Ile:Lys (44, 51, 57, 63, and 70%), 1.18% SID Leu, and 0.90% SID Lys (second limiting). Diet 6 (diet 5 with added Lys) was formulated (1.06% SID Lys) as a positive control. Pigs fed diet 6 had higher (P < 0.05) ADG and G:F and lower (P < 0.05) plasma urea N (PUN) than pigs fed diet 5 (P < 0.02), indicating that Lys was limiting in diets 1 to 5. Final BW, ADG, and ADFI increased (linear and quadratic, P < 0.05) while G:F and PUN at d 21 were not affected (P > 0.10) by dietary Ile:Lys. Overall, ADG and ADFI were highest for pigs fed diet 2 (51% SID Ile:Lys). In Exp. 2, 216 Yorkshire pigs (initial BW = 9.6 kg) were assigned to 9 diets with 6 pens per treatment. Diets 1 to 4 contained 0.40, 0.47, 0.54, and 0.61% SID Ile, respectively, and 1.21% SID Lys; diets 5 to 8 contained 0.72, 0.84, 0.96, and 1.08% SID Lys, respectively, and 0.68% SID Ile. Diet 9 was high in both Ile and Lys (0.68% SID Ile and 1.21% SID Lys). All diets contained 1.21% SID Leu. The ADG and G:F increased (linear and quadratic, P < 0.05) as SID Ile:Lys increased (diets 1 to 4 and 9). The ADG and G:F increased (linear, P < 0.05) as SID Lys increased (diets 5 to 9). The PUN at d 21 decreased (linear, P < 0.05) by increasing dietary Ile:Lys. The SID Ile:Lys to optimize ADG was 46% by curvilinear plateau or exponential regression. For G:F, the optimal SID Ile:Lys was 47 and 51% by curvilinear plateau and exponential regressions, respectively. In Exp. 3, 80 pigs (PIC 327 x C23; initial BW = 24.0 kg) were allotted to 5 treatments with 4 pigs per pen. Diets 1 to 5 were formulated to contain 5 graded SID Ile:Lys (39, 46, 53, 61, and 68%), 1.17% SID Leu, and 0.91% SID Lys (second limiting). Final BW and ADG increased (linear and quadratic, P < 0.05) and ADFI increased (linear, P = 0.047) as SID Ile:Lys increased. Using ADG and G:F, the optimum SID Ile:Lys was 54 and 53%, respectively, by curvilinear plateau and exponential regression. The PUN was minimized at 53 and 59% SID Ile:Lys by curvilinear plateau and broken line regression. Overall, the average optimum SID Ile:Lys was approximately 51% for 10- to 22-kg pigs and 54% for 24- to 39-kg pigs fed diets containing nonexcess levels of Leu. C1 [Htoo, J. K.] Evonik Ind AG, D-63457 Hanau, Germany. [Zhu, C. L.; Huber, L.; de lange, C. F. M.] Univ Guelph, Dept Anim & Poultry Sci, Guelph, ON N1G 2W1, Canada. [Quant, A. D.; Cromwell, G. L.; Lindemann, M. D.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Kerr, B. J.] USDA ARS, Natl Lab Agr & Environm, Ames, IA 50011 USA. RP Htoo, JK (reprint author), Evonik Ind AG, Rodenbacher Chaussee 4, D-63457 Hanau, Germany. EM john.htoo@evonik.com NR 27 TC 2 Z9 2 U1 1 U2 6 PU AMER SOC ANIMAL SCIENCE PI CHAMPAIGN PA PO BOX 7410, CHAMPAIGN, IL 61826-7410 USA SN 0021-8812 EI 1525-3163 J9 J ANIM SCI JI J. Anim. Sci. PD AUG PY 2014 VL 92 IS 8 BP 3482 EP 3490 DI 10.2527/jas2013-6934 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AO9GH UT WOS:000341663600029 PM 24987070 ER PT J AU Islam, MS Zeng, LH Delhom, CD Song, XL Kim, HJ Li, P Fang, DD AF Islam, Md S. Zeng, Linghe Delhom, Christopher D. Song, Xianliang Kim, Hee Jin Li, Ping Fang, David D. TI Identification of cotton fiber quality quantitative trait loci using intraspecific crosses derived from two near-isogenic lines differing in fiber bundle strength SO MOLECULAR BREEDING LA English DT Article DE Cotton; Fiber quality; Fiber strength; Microsatellite markers; QTL ID X GOSSYPIUM-BARBADENSE; SIMPLE SEQUENCE REPEAT; HIRSUTUM L. CULTIVARS; UPLAND COTTON; TETRAPLOID COTTON; QTL ANALYSIS; GENETIC-MAP; BACKCROSS POPULATION; RIL POPULATION; G. BARBADENSE AB Cotton fiber properties are very important to the yarn quality. Modern high-speed textile operations around the world require long, strong and fine cotton fibers. The objective of this research was to identify stable fiber quantitative trait loci (QTLs) that could be used in cotton breeding through marker-assisted selection (MAS). Two cotton lines, MD90ne and MD52ne, are near-isogenic with significant differences in fiber properties, especially strength. Fiber samples from 734 progeny plants of two F2 populations (A and B) derived from crosses between MD90ne and MD52ne were collected at Stoneville, MS, USA in 2012. Fiber quality attributes were measured using a High Volume Instrument 1000. A simple sequence repeat (SSR) genetic linkage map with 165 loci covering 632.53 cM was constructed using population A, consisting of 356 F2 individuals and used for identifying QTLs related to fiber bundle strength (FBS), short fiber index (SFI) and upper-half mean fiber length (UHML). One QTL for FBS originating from the stronger fiber parent MD52ne was identified on chromosome (Chr.) 3. Three QTLs each for SFI and UHML were identified on Chrs. 3, 4 and 14 and Chrs. 3, 11 and 24, respectively. Population B, consisting of 378 F2 progeny, was used to confirm these QTLs by analyzing 57 SSR markers mapped on Chrs. 3, 14 and 24. Three QTLs-qFBS-c3, qSFI-c14 and qUHML-c24-were confirmed and appeared stable. These three QTLs could potentially be used in breeding to improve cotton fiber quality through a MAS strategy. C1 [Islam, Md S.; Song, Xianliang; Kim, Hee Jin; Li, Ping; Fang, David D.] ARS, Cotton Fiber Biosci Res Unit, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Zeng, Linghe] ARS, Crop Genet Res Unit, USDA, Stoneville, MS 38772 USA. [Delhom, Christopher D.] ARS, Cotton Struct & Qual Res Unit, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. [Song, Xianliang] Shandong Agr Univ, State Key Lab Crop Biol, Coll Agron, Tai An 271018, Shandong, Peoples R China. RP Fang, DD (reprint author), ARS, Cotton Fiber Biosci Res Unit, USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. EM david.fang@ars.usda.gov OI fang, david/0000-0003-0036-5459 FU USDA-ARS CRIS [6435-21000-017-00D]; Cotton Incorporated project [10-747] FX This project was supported by USDA-ARS CRIS project 6435-21000-017-00D and Cotton Incorporated project 10-747. We greatly thank Dr. William Meredith, who developed the cotton lines MD90ne and MD52ne and made them available for us to use in the present research. Our appreciation also goes to Mrs. Sheron Simpson and Dr. Brian Scheffler at Genomic and Bioinformatic Research Unit at Stoneville, MS for their excellent support in SSR marker analysis. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture, which is an equal opportunity provider and employer. NR 43 TC 12 Z9 12 U1 4 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2014 VL 34 IS 2 BP 373 EP 384 DI 10.1007/s11032-014-0040-4 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AP6CI UT WOS:000342164700009 ER PT J AU Gillman, JD Tetlow, A Hagely, K Boersma, JG Cardinal, A Rajcan, I Bilyeu, K AF Gillman, Jason D. Tetlow, Ashley Hagely, Katherine Boersma, Jeffery G. Cardinal, Andrea Rajcan, Istvan Bilyeu, Kristin TI Identification of the molecular genetic basis of the low palmitic acid seed oil trait in soybean mutant line RG3 and association analysis of molecular markers with elevated seed stearic acid and reduced seed palmitic acid SO MOLECULAR BREEDING LA English DT Article DE Soybean; Oil improvement; Palmitic acid; Stearic acid; Mutagenesis ID INHERITANCE; MUTATIONS; CHOLESTEROL AB The fatty acid composition of vegetable oil is becoming increasingly critical for its ultimate functionality and utilization in foods and industrial products. Partial chemical hydrogenation of soybean [Glycine max (L.) Merr.] oil increases oxidative stability and shelf life but also results in the introduction of trans fats as an unavoidable byproduct. Due to mandatory labeling of consumer products containing trans fats, conventional soybean oil has lost the ability to deliver the most appropriate economical functionality and oxidative stability, particularly for baking applications. Genetic improvement of the fatty acid profile of soybean oil is one method of meeting these new requirements for oil feedstocks. In this report, we characterized three mutant genetic loci controlling the saturated fatty acid content of soybean oil: two genes additively reduce palmitic acid content (fap1 and fap3-ug), and one gene independently elevates stearic acid content (fas). We identified a new null allele of fap3-ug/GmFATB1A (derived from line ELLP2) present in line RG3. The splicing defect mutation in a beta-ketoacyl-[acyl-carrier-protein] synthase III candidate gene located in the region mapped to fap1, derived originally from ethyl methane sulphonate mutant line C1726 (Cardinal et al. in Theor Appl Genet 127:97-111,2014), was also present in line RG3. We also utilized the elevated stearic acid line RG7, which has previously been shown to contain novel mutant fas/SACPD-C alleles encoding stearoyl-acyl carrier protein desaturase (Boersma et al. in Crop Sci 52:1736-1742,2012). Molecular marker assays have been developed to track these causative mutations and understand their contributions to seed oil fatty acid profiles in a recombinant inbred line population segregating for fap1, fap3-ug, and fas alleles. C1 [Gillman, Jason D.; Bilyeu, Kristin] Univ Missouri, USDA ARS, Plant Genet Res Unit, Columbia, MO 65211 USA. [Tetlow, Ashley; Hagely, Katherine] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Boersma, Jeffery G.; Rajcan, Istvan] Univ Guelph, Dept Plant Agr, Guelph, ON N1G 2W1, Canada. [Cardinal, Andrea] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Bilyeu, K (reprint author), Univ Missouri, USDA ARS, Plant Genet Res Unit, 110 Waters Hall, Columbia, MO 65211 USA. EM bilyeuk@missouri.edu NR 23 TC 4 Z9 4 U1 3 U2 24 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2014 VL 34 IS 2 BP 447 EP 455 DI 10.1007/s11032-014-0046-y PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AP6CI UT WOS:000342164700015 ER PT J AU Zhang, HL Huang, JZ Liu, QL Nawaz, Z Lu, HP Gong, JY Zhu, YJ Yan, WG Shu, QY AF Zhang, Hua-Li Huang, Jian-Zhong Liu, Qing-Long Nawaz, Zarqa Lu, Hai-Ping Gong, Jun-Yi Zhu, Yu-Jun Yan, Wengui Shu, Qing-Yao TI Characterization of an RNase Z nonsense mutation identified exclusively in environment-conditioned genic male sterile rice SO MOLECULAR BREEDING LA English DT Article DE Hybrid rice; Gene sequencing; Molecular marker; Plant breeding; Rice ID HYBRID RICE; NONCODING RNA; GENES; TMS5 AB The two-line hybrid system in rice is becoming more important and employs environment-conditioned genic male sterile (EGMS) lines sensitive to photoperiod (photoperiod-sensitive genic male sterile), temperature [temperature genic male sterile (TGMS)], or a combination of the two (photoperiod temperature genic male sterile). At least 18 EGMS genes have been mapped, and two cloned, but controversies exist. For example, three different genes were reported to underlie the TGMS trait in three independently identified progenitors, Annong S-1, Zhu 1S, and Guangzhan 63S, while another study demonstrated that the TGMS genes in Annong S-1 and Zhu 1S are allelic. In the present study, we confirmed the allelism of the three TGMS genes, which means there is a common TGMS gene(s) in these lines. Knowing there is an association between the mutant allele (RNZ(m)) of a ribonuclease gene (RNZ) with the TGMS trait in Guangzhuan 63S, we then sequenced RNZ for 14 commercial EGMS and 21 non-EGMS lines, and we developed two derived cleaved amplified polymorphic sequence (dCAPS) markers to detect RNZ(m) alleles in 32 EGMS and 310 non-EGMS lines. The analyses showed that the RNZ(m) allele existed exclusively in EGMS lines; all non-EGMS lines contained the functional RNZ(gc) or RNZ(tc) allele. Furthermore, two segregating populations that included 2,429 individuals were developed by crossing Zhu 1S (RNZ(m)) to two non-EGMSlines (both with RNZ(tc)); examination of the segregation of male sterile and fertile plants indicated that the TGMS trait was under the control of a single gene; analysis of the markers revealed the RNZ(m) allele exclusively in TGMS plants and the RNZ(tc) allele only in non-TGMS plants in both populations. The dCAPS markers could therefore help select TGMS progeny in breeding programs, which will save time and labor, and improve breeding efficiency and accuracy. C1 [Zhang, Hua-Li; Huang, Jian-Zhong; Nawaz, Zarqa; Lu, Hai-Ping; Shu, Qing-Yao] Zhejiang Univ, State Key Lab Rice Biol, Hangzhou 310029, Peoples R China. [Zhang, Hua-Li; Huang, Jian-Zhong; Nawaz, Zarqa; Lu, Hai-Ping; Shu, Qing-Yao] Zhejiang Univ, Chinese Minist Agr Nucl Agr Sci, Key Lab, Hangzhou 310029, Peoples R China. [Zhang, Hua-Li; Huang, Jian-Zhong; Nawaz, Zarqa; Lu, Hai-Ping; Shu, Qing-Yao] Zhejiang Univ, Inst Nucl Agr Sci, Hangzhou 310029, Peoples R China. [Zhang, Hua-Li; Lu, Hai-Ping; Shu, Qing-Yao] Wuxi Qiushi Agri Biol Res Ctr, Wuxi 214105, Jiangsu, Peoples R China. [Liu, Qing-Long] Zhejiang Acad Agr Sci, Inst Crop Sci & Nucl Technol Utilizat, Hangzhou 310021, Zhejiang, Peoples R China. [Gong, Jun-Yi; Zhu, Yu-Jun] China Natl Rice Res Inst, Hangzhou 310006, Zhejiang, Peoples R China. [Yan, Wengui] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. RP Shu, QY (reprint author), Zhejiang Univ, State Key Lab Rice Biol, Hangzhou 310029, Peoples R China. EM qyshu@zju.edu.cn RI Shu, Qing-Yao/C-4859-2008; OI Shu, Qing-Yao/0000-0002-9201-0593; Zhu, Yu-Jun/0000-0001-6409-1990 FU Special Funds for Agro-scientific Research in the Public Interest [201103007]; Zhejiang 8812 Program; Wuxi Science and Technology Department [CYES1002]; IAEA [16818] FX The research was financially supported by the Special Funds for Agro-scientific Research in the Public Interest (201103007) and the Zhejiang 8812 Program, and in part by the grant of Wuxi Science and Technology Department (#CYES1002) and by the IAEA coordinated research project # 16818. We thank Drs. Thomas Tai (USDA-ARS) and Yuwei Shen (DNA Landmarks) for critical comments to help improve the manuscript. NR 28 TC 4 Z9 7 U1 4 U2 12 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2014 VL 34 IS 2 BP 481 EP 489 DI 10.1007/s11032-014-0051-1 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AP6CI UT WOS:000342164700018 ER PT J AU Nimmakayala, P Abburi, VL Bhandary, A Abburi, L Vajja, VG Reddy, R Malkaram, S Venkatramana, P Wijeratne, A Tomason, YR Levi, A Wehner, TC Reddy, UK AF Nimmakayala, Padma Abburi, Venkata Lakshmi Bhandary, Abhishek Abburi, Lavanya Vajja, Venkata Gopinath Reddy, Rishi Malkaram, Sridhar Venkatramana, Pegadaraju Wijeratne, Asela Tomason, Yan R. Levi, Amnon Wehner, Todd C. Reddy, Umesh K. TI Use of VeraCode 384-plex assays for watermelon diversity analysis and integrated genetic map of watermelon with single nucleotide polymorphisms and simple sequence repeats SO MOLECULAR BREEDING LA English DT Article DE SNPs; High throughput genotyping; SSRs; Genetic mapping; Watermelon; Cultivar diversity ID CITRULLUS-LANATUS; SNP DISCOVERY; LINKAGE MAP; GENOME; ACCESSIONS; MARKERS; COLOCYNTHIS; NAKAI AB Watermelon (Citrullus lanatus var. lanatus) is one of the most important vegetable crops in the world. Molecular markers have become the tools of choice for resolving watermelon taxonomic relationships and evolution. Increased numbers of single nucleotide polymorphism (SNP) markers together with simple sequence repeat (SSR) markers would be useful for phylogenetic analyses of germplasm accessions and for linkage mapping for marker-assisted breeding with quantitative trait loci and single genes. We aimed to construct a genetic map based on SNPs (generated by Illumina Veracode multiplex assays for genotyping) and SSR markers and evaluate relationships inferred from SNP genotypes between 130 watermelon accessions collected throughout the world. We incorporated 282 markers (232 SNPs and 50 SSRs) into the linkage map. The genetic map consisted of 11 linkage groups spanning 924.72 cM with an average distance of 3.28 cM between markers. Because all of the SNP-containing sequences were assembled with the whole-genome sequence draft for watermelon, chromosome numbers could be readily assigned for all the linkage groups. We found that 134 SNPs were polymorphic in 130 watermelon accessions chosen for diversity studies. The current 384-plex SNP set is a powerful tool for characterizing genetic relatedness and for developing medium-resolution genetic maps. C1 [Nimmakayala, Padma; Abburi, Venkata Lakshmi; Bhandary, Abhishek; Abburi, Lavanya; Vajja, Venkata Gopinath; Reddy, Rishi; Malkaram, Sridhar; Tomason, Yan R.; Reddy, Umesh K.] West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. [Venkatramana, Pegadaraju] BioDiagnostics Inc, River Falls, WI 54022 USA. [Wijeratne, Asela] Ohio State Univ, Ohio Agr Res & Dev Ctr, Mol & Cellular Imagining Ctr, Wooster, OH 44691 USA. [Levi, Amnon] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Wehner, Todd C.] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. RP Reddy, UK (reprint author), West Virginia State Univ, Gus R Douglass Inst, Dept Biol, Institute, WV 25112 USA. EM ureddy@wvstateu.edu FU USDA-NIFA [2013-38821-21453]; National Science Foundation [NSF 09-570 - EPS-1003907]; Gus R. Douglass Institute; NIH [P20RR016477] FX This project was supported by the USDA-NIFA (no. 2013-38821-21453), National Science Foundation under Grant No. NSF 09-570 - EPS-1003907, Gus R. Douglass Institute and NIH Grant P20RR016477 to the West Virginia IDeA Network for Biomedical Research Funding. The authors thank GRDI graduate assistantships for A. Bhandary and L. Abburi. The authors are grateful to R. Jarret, Plant Genetic Resources Conservation Unit, USDA-ARS (Griffin, GA) for providing the seeds of germplasm accessions. Conflict of interestWe declare that we have no conflict of interest. NR 54 TC 6 Z9 6 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2014 VL 34 IS 2 BP 537 EP 548 DI 10.1007/s11032-014-0056-9 PG 12 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AP6CI UT WOS:000342164700023 ER PT J AU Bian, Y Ballington, J Raja, A Brouwer, C Reid, R Burke, M Wang, XG Rowland, LJ Bassil, N Brown, A AF Bian, Yang Ballington, James Raja, Archana Brouwer, Cory Reid, Robert Burke, Mark Wang, Xinguo Rowland, Lisa J. Bassil, Nahla Brown, Allan TI Patterns of simple sequence repeats in cultivated blueberries (Vaccinium section Cyanococcus spp.) and their use in revealing genetic diversity and population structure SO MOLECULAR BREEDING LA English DT Article DE Blueberry; Vaccinium; Genetic diversity; SSR; STRUCTURE ID MULTILOCUS GENOTYPE DATA; EST-PCR MARKERS; HIGHBUSH BLUEBERRY; MICROSATELLITE MARKERS; COLD-ACCLIMATION; SSR-MARKERS; LOWBUSH BLUEBERRY; SATIVA L.; DNA; EVOLUTION AB Blueberry (Vaccinium section Cyanococcus) is an important small fruit crop native to North America. Relationships among the primary species known as 'blueberry' has been a source of speculation due to the out-breeding nature of the crop, the use of intra-specific hybridization and open-pollinated selection in breeding programs, and the lack of genomic resources to adequately address the issue. The objectives of this study were to characterize simple sequence repeats (SSRs) from an emerging genomic draft sequence, develop useful molecular markers and provide an in-depth analysis of genetic diversity and population structure in blueberry using a broad range of cultivated blueberry accessions representing multiple species, ploidy levels and sources of origin. Genomic scaffolding was assembled from the whole-genome sequencing of a diploid V. corymbosum accession 'W8520.' From the assembled 358 Mb sequence, a total number of 43,594 SSRs were identified (122 per Mb). Among genomic regions, SSRs were longest and occurred most frequently in predicted 50 untranslated regions (5' UTR), while SSRs were shortest and least common in the predicted coding sequences. AG/CT and AAG/CTT were the most frequent motifs while CG/CG and CCG/CGG motifs were the rarest di- and trinucleotide motifs, respectively. For analysis of genetic diversity and population structure, 42 genomic SSR and EST-SSR markers with an average of 14.2 alleles and 56.0 allele phenotypes per locus were used to genotype a diverse blueberry population of 150 accessions. Cluster analysis grouped the 150 accessions in a manner consistent with known information regarding species, ploidy levels and pedigree. The analysis of population structure among blueberry accessions revealed inter- and intra-specific levels of stratification. Rabbiteye blueberry (V. virgatum) represents a genetically distinct subgroup within Cyanococcus. Three additional subpopulations were detected among highbush varieties that are largely attributable to distinctions between northern and southern highbush and founder effects of a single cultivar ('Weymouth'). The identification of substructure that correlates with known pedigree information, and the availability of new genomic molecular markers will facilitate future evolutionary and genetic studies in blueberry. C1 [Bian, Yang; Ballington, James; Brown, Allan] N Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA. [Bian, Yang; Raja, Archana; Brown, Allan] N Carolina State Univ, Plants Human Hlth Inst, Kannapolis, NC 28081 USA. [Brouwer, Cory; Reid, Robert] Univ N Carolina Charlotte, Bioinformat Serv Div, Kannapolis, NC USA. [Burke, Mark; Wang, Xinguo] David H Murdock Res Inst, Kannapolis, NC USA. [Rowland, Lisa J.] USDA ARS, Genet Improvement Fruits & Vegetables Lab, BARC West, Beltsville, MD 20705 USA. [Bassil, Nahla] USDA ARS, Natl Clonal Germplasm Repository, Corvallis, OR 97333 USA. RP Brown, A (reprint author), N Carolina State Univ, Plants Human Hlth Inst, North Carolina Res Campus,600 Laureate Way, Kannapolis, NC 28081 USA. EM afbrown2@ncsu.edu OI Reid, Robert/0000-0001-7125-7151 FU University of North Carolina General Administration Fund FX The authors are grateful to Dr. Mark Ehlenfeldt for providing the coefficients of coancestry. The authors also thank Dr. James Olmstead for providing some DNA materials. Drs. Gad Yousef and Ivette Guzman, and Phuc (Peter) Pham are kindly acknowledged for technical assistance. This research was supported by a grant from the University of North Carolina General Administration Fund. NR 67 TC 9 Z9 9 U1 7 U2 41 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1380-3743 EI 1572-9788 J9 MOL BREEDING JI Mol. Breed. PD AUG PY 2014 VL 34 IS 2 BP 675 EP 689 DI 10.1007/s11032-014-0066-7 PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AP6CI UT WOS:000342164700032 ER PT J AU Weaver, CP Mooney, S Allen, D Beller-Simms, N Fish, T Grambsch, AE Hohenstein, W Jacobs, K Kenney, MA Lane, MA Langner, L Larson, E McGinnis, DL Moss, RH Nichols, LG Nierenberg, C Seyller, EA Stern, PC Winthrop, R AF Weaver, C. P. Mooney, S. Allen, D. Beller-Simms, N. Fish, T. Grambsch, A. E. Hohenstein, W. Jacobs, K. Kenney, M. A. Lane, M. A. Langner, L. Larson, E. McGinnis, D. L. Moss, R. H. Nichols, L. G. Nierenberg, C. Seyller, E. A. Stern, P. C. Winthrop, R. TI From global change science to action with social sciences SO NATURE CLIMATE CHANGE LA English DT Editorial Material ID CLIMATE ADAPTATION C1 [Weaver, C. P.; Grambsch, A. E.] US EPA, Washington, DC 20460 USA. [Mooney, S.] Boise State Univ, Dept Econ, Boise, ID 83725 USA. [Mooney, S.] Natl Sci Fdn, Expt Program Stimulate Competit Res, Arlington, VA 22230 USA. [Allen, D.; Seyller, E. A.] US Global Change Res Program, Washington, DC 20006 USA. [Beller-Simms, N.; Nierenberg, C.] NOAA, Silver Spring, MD 20910 USA. [Fish, T.] US Dept Interior, Washington, DC 20240 USA. [Hohenstein, W.] USDA, Washington, DC 20250 USA. [Jacobs, K.] Univ Arizona, Ctr Climate Adaptat Sci & Solut, Tucson, AZ 85721 USA. [Jacobs, K.] Univ Arizona, Dept Soil Water & Environm Sci, Tucson, AZ 85721 USA. [Kenney, M. A.] Univ Maryland, Cooperat Inst Climate & Satellites Maryland, Earth Syst Sci Interdisciplinary Ctr, College Pk, MD 20740 USA. [Lane, M. A.; Stern, P. C.] CNR, Board Environm Change & Soc, Washington, DC 20001 USA. [Langner, L.] US Forest Serv, USDA, Washington, DC 20250 USA. [Larson, E.] NASA, Washington, DC 20546 USA. [McGinnis, D. L.] Montana State Univ, Billings, MT 59101 USA. [Moss, R. H.] Pacific NW Natl Lab, Joint Global Change Res Inst, College Pk, MD 20740 USA. [Moss, R. H.] Univ Maryland, College Pk, MD 20740 USA. [Nichols, L. G.] Natl Sci Fdn, Div Behav & Cognit Sci, Arlington, VA 22230 USA. [Winthrop, R.] Bureau Land Management, Washington, DC 20003 USA. RP Weaver, CP (reprint author), US EPA, 1200 Penn Ave NW, Washington, DC 20460 USA. EM weaver.chris@epa.gov RI Weaver, Christopher/G-3714-2010 OI Weaver, Christopher/0000-0003-4016-5451 NR 22 TC 30 Z9 30 U1 1 U2 27 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1758-678X EI 1758-6798 J9 NAT CLIM CHANGE JI Nat. Clim. Chang. PD AUG PY 2014 VL 4 IS 8 BP 656 EP 659 PG 4 WC Environmental Sciences; Environmental Studies; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AO7YF UT WOS:000341568200010 ER PT J AU Baidya, S Duran, RM Lohmar, JM Harris-Coward, PY Cary, JW Hong, SY Roze, LV Linz, JE Calvo, AM AF Baidya, Sachin Duran, Rocio M. Lohmar, Jessica M. Harris-Coward, Pamela Y. Cary, Jeffrey W. Hong, Sung-Yong Roze, Ludmila V. Linz, John E. Calvo, Ana M. TI VeA Is Associated with the Response to Oxidative Stress in the Aflatoxin Producer Aspergillus flavus SO EUKARYOTIC CELL LA English DT Article ID OSMOTIC-STRESS; SECONDARY METABOLISM; SEXUAL DEVELOPMENT; TRANSCRIPTIONAL ACTIVATION; SCLEROTIAL PRODUCTION; OXIDANT ADAPTATION; NIDULANS; GROWTH; GENES; PARASITICUS AB Survival of fungal species depends on the ability of these organisms to respond to environmental stresses. Osmotic stress or high levels of reactive oxygen species (ROS) can cause stress in fungi resulting in growth inhibition. Both eukaryotic and prokaryotic cells have developed numerous mechanisms to counteract and survive the stress in the presence of ROS. In many fungi, the HOG signaling pathway is crucial for the oxidative stress response as well as for osmotic stress response. This study revealed that while the osmotic stress response is only slightly affected by the master regulator veA, this gene, also known to control morphological development and secondary metabolism in numerous fungal species, has a profound effect on the oxidative stress response in the aflatoxin-producing fungus Aspergillus flavus. We found that the expression of A. flavus homolog genes involved in the HOG signaling pathway is regulated by veA. Deletion of veA resulted in a reduction in transcription levels of oxidative stress response genes after exposure to hydrogen peroxide. Furthermore, analyses of the effect of VeA on the promoters of cat1 and trxB indicate that the presence of VeA alters DNA-protein complex formation. This is particularly notable in the cat1 promoter, where the absence of VeA results in abnormally stronger complex formation with reduced cat1 expression and more sensitivity to ROS in a veA deletion mutant, suggesting that VeA might prevent binding of negative transcription regulators to the cat1 promoter. Our study also revealed that veA positively influences the expression of the transcription factor gene atfB and that normal formation of DNA-protein complexes in the cat1 promoter is dependent on AtfB. C1 [Baidya, Sachin; Duran, Rocio M.; Lohmar, Jessica M.; Calvo, Ana M.] No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. [Harris-Coward, Pamela Y.; Cary, Jeffrey W.] USDA ARS, So Reg Res Ctr, New Orleans, LA USA. [Hong, Sung-Yong; Roze, Ludmila V.; Linz, John E.] Michigan State Univ, Dept Microbiol & Mol Genet, Dept Food Sci & Human Nutr, E Lansing, MI USA. RP Calvo, AM (reprint author), No Illinois Univ, Dept Biol Sci, De Kalb, IL 60115 USA. EM amcalvo@niu.edu FU Department of Biological Sciences at NIU; [USDA-SCA 58-6435-9-386] FX This project was funded by USDA-SCA 58-6435-9-386 and the Department of Biological Sciences at NIU. NR 47 TC 13 Z9 14 U1 2 U2 21 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 1535-9778 EI 1535-9786 J9 EUKARYOT CELL JI Eukaryot. Cell PD AUG PY 2014 VL 13 IS 8 BP 1095 EP 1103 DI 10.1128/EC.00099-14 PG 9 WC Microbiology; Mycology SC Microbiology; Mycology GA AO8DG UT WOS:000341582800014 PM 24951443 ER PT J AU Chakraborty, N Sim, SC Casler, M Jung, G AF Chakraborty, Nanda Sim, Sung-Chur Casler, Michael Jung, Geunhwa TI Development of SCAR marker for the detection of QTL for resistance to dollar spot in creeping bentgrass (Agrostis stolonifera L.) SO HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY LA English DT Article DE disease resistance; molecular markers; SCAR ID SCLEROTINIA-HOMOEOCARPA; GOLF-COURSES; RAPD; SENSITIVITY; CULTIVARS; GENE; IDENTIFICATION; BENZIMIDAZOLE; POPULATIONS; FUNGICIDES AB Dollar spot (caused by Sclerotinia homoeocarpa F. T. Bennett) is the most economically important fungal turf disease on golf courses in North America. This disease is mainly controlled by using multiple fungicides. However, the causal fungus has developed resistance to seveal classes of fungicides and host resistance is an alternative control strategy. A creeping bentgrass (Agrostis stolonifera L.) cultivar improved with dollar spot resistance would greatly reduce the costs and environmental impacts of fungicide applications. Our previous QTL analysis in the creeping bentgrass '549 x 372' population detected one QTL with a large effect for dollar spot resistance and several QTLs with smaller effects. One RAPD primer, AW10, amplified an 650-bp band in the parent '372', was named 3.AW10.650. This RAPD marker was located on linkage group 7.1 and was significantly (p < 0.0001) associated with the QTL for dollar spot resistance based on 697 progeny of the '549 x 372' population. In this study, we converted the RAPD marker 3.AW10.650 into a sequence characterized amplified region (SCAR) marker, named CreepDSp.650. The SCAR marker showed a significant association (p = 0.006) with dollar spot resistance in an additional segregating population developed at the University of Illinois. We report here the first PCR-based SCAR marker associated with a major-effect QTL for dollar spot resistance in creeping bentgrass. This SCAR marker will facilitate evaluating relatively large numbers of breeding progeny for marker-assisted selection (MAS) in bentgrasses. C1 [Chakraborty, Nanda] Syngenta Seeds Inc, Stanton, MN 55018 USA. [Sim, Sung-Chur] Sejong Univ, Dept Bioresources Engn, Seoul 143747, South Korea. [Casler, Michael] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Jung, Geunhwa] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. RP Jung, G (reprint author), Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA. EM jung@umass.edu FU Hatch Formula Fund [WIS04777]; Wisconsin Turfgrass Association; U.S. Department of Agriculture (USDA) FX We thank Mark Manemann for assistance with DNA extraction. Funding for this project was provided by Hatch Formula Fund (WIS04777), the Wisconsin Turfgrass Association and U.S. Department of Agriculture (USDA) Research Cooperative Agreement. NR 29 TC 2 Z9 2 U1 1 U2 8 PU KOREAN SOC HORTICULTURAL SCIENCE PI SUWON PA C/O NATL HORTICULTURAL RES INST, IMOK-DONG 475, JANGAN-GU, SUWON, 440-706, SOUTH KOREA SN 2211-3452 EI 2211-3460 J9 HORTIC ENVIRON BIOTE JI Hortic. Environ. Biotechnol. PD AUG PY 2014 VL 55 IS 4 BP 329 EP 334 DI 10.1007/s13580-014-0015-8 PG 6 WC Horticulture SC Agriculture GA AP1KV UT WOS:000341829600011 ER PT J AU Xue, XY Tu, K Qin, WC Lan, YB Zhang, HH AF Xue Xinyu Tu Kang Qin Weicai Lan, Yubin Zhang, Huihui TI Drift and deposition of ultra-low altitude and low volume application in paddy field SO INTERNATIONAL JOURNAL OF AGRICULTURAL AND BIOLOGICAL ENGINEERING LA English DT Article DE paddy field; ultra-low altitude; low volume; UAV; droplet drift; deposition AB Field trials were performed to evaluate various techniques for measuring spray deposition and aerial drift during spray application to paddy field. The application of a spraying agent containing the fluorescent dye Rhodamine-B was applied by an unmanned aerial vehicle (UAV) which flew at a height of 5 m, a speed of 3 m/s, and the wind speed of 3 m/s. The results showed that because the downdraft produced by a helicopter rotor increased the penetrability of crops, there is a higher deposition on the upper layer and the under layer than the traditional spraying. The average deposition on the upper layer accounts for 28% of the total spraying, the deposition on the under layer accounts for 26% of the total spraying. The deposition on the under layer takes up 92.8% of the deposition on the upper layer. Droplets drift data showed that the drift of non-target area took up 12.9% of the total liquid spray. The 90% drifting droplets were located within a range of 8 m of the target area; the drift quantity was almost zero at a distance of 50 m away from the treated area. C1 [Xue Xinyu; Tu Kang] Nanjing Agr Univ, Nanjing 210014, Jiangsu, Peoples R China. [Xue Xinyu; Qin Weicai] Minist Agr, Nanjing Res Inst Agr Mechanizat, Nanjing 210014, Jiangsu, Peoples R China. [Lan, Yubin] South China Agr Univ, Guangzhou 510642, Guangdong, Peoples R China. [Zhang, Huihui] USDA ARS, Ft Collins, CO 80526 USA. RP Tu, K (reprint author), Nanjing Agr Univ, Nanjing 210014, Jiangsu, Peoples R China. EM 735178312@qq.com; kangtu@njau.edu.cn; qinweicai@caas.cn; ylan@scau.edu.cn; huihui.zhang@ars.usda.gov FU Special Fund for Agro-scientific Research in the Public Interest, Ministry of Agriculture, China [201203025]; National High Technology Research and Development Program of China [SS2013AA100303] FX The authors gratefully acknowledge the financial support provided by 1) Special Fund for Agro-scientific Research in the Public Interest (201203025) by Ministry of Agriculture, China and 2) the National High Technology Research and Development Program of China (SS2013AA100303). NR 15 TC 5 Z9 8 U1 6 U2 33 PU CHINESE ACAD AGRICULTURAL ENGINEERING PI BEIJING PA RM 506, 41, MAIZIDIAN ST, CHAOYANG DISTRICT, BEIJING, 100125, PEOPLES R CHINA SN 1934-6344 EI 1934-6352 J9 INT J AGR BIOL ENG JI Int. J. Agric. Biol. Eng. PD AUG PY 2014 VL 7 IS 4 BP 23 EP 28 DI 10.3965/j.ijabe.20140704.003 PG 6 WC Agricultural Engineering SC Agriculture GA AO7TU UT WOS:000341556300004 ER PT J AU Jong, L AF Jong, Lei TI Effect of processing methods on the mechanical properties of natural rubber filled with stearic acid-modified soy protein particles SO JOURNAL OF ELASTOMERS AND PLASTICS LA English DT Article DE Rubber; mechanical; soy; composites; reinforcement ID COMPOSITES; BEHAVIOR; ISOLATE AB Natural rubber was reinforced with stearic acid-modified soy protein particles prepared using a microfluidizing and ball milling process. Longer ball milling time tends to increase the tensile strength of the rubber composites. Elastic modulus of the composites increased with the increasing filler concentration. The loss modulus and loss tangent indicated an increase in the amount of polymer immobilized by the modified soy protein. The extent of stearic acid modification affected the mechanical properties of the rubber composites. Compared with the unmodified soy protein, the microfluidized and stearic acid-modified soy protein filler improved the mechanical properties of the rubber composites. C1 USDA, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA. RP Jong, L (reprint author), USDA, Natl Ctr Agr Utilizat Res, 1815N Univ St, Peoria, IL 61604 USA. EM lei.jong@ars.usda.gov NR 18 TC 1 Z9 1 U1 0 U2 13 PU SAGE PUBLICATIONS LTD PI LONDON PA 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND SN 0095-2443 EI 1530-8006 J9 J ELASTOM PLAST JI J. Elastomer Plast. PD AUG PY 2014 VL 46 IS 5 BP 413 EP 426 DI 10.1177/0095244312469962 PG 14 WC Materials Science, Multidisciplinary; Polymer Science SC Materials Science; Polymer Science GA AP1XS UT WOS:000341866200002 ER PT J AU Jenkins, MC Widmer, G O'Brien, C Bauchan, G Murphy, C Santin, M Fayer, R AF Jenkins, Mark C. Widmer, Giovanni O'Brien, Celia Bauchan, Gary Murphy, Charles Santin, Monica Fayer, Ronald TI A Highly Divergent 33 kDa Cryptosporidium parvum Antigen SO JOURNAL OF PARASITOLOGY LA English DT Article ID HOMINIS; GENOME; PROTEINS; FEATURES; OOCYSTS AB Previous studies comparing the genome sequences of Cryptosporidium parvum with Cryptosporidium hominis identified a number of highly divergent genes that might reflect positive selection for host specificity. In the present study, the C. parvum DNA sequence cgd8-5370, which encodes a protein whose amino acid sequence differs appreciably from its homologue in C. hominis, was cloned by PCR and expressed as a recombinant protein in Escherichia coli. Antisera raised against the recombinant cgd8-5370 antigen strongly recognized a unique 33 kDa protein in immunoblots from reducing and non-reducing SDS-PAGE of native C. parvum protein. However, anti-Cp33 sera did not recognize the native 33 kDa homologue in C. hominis. In an immunofluorescence assay (IFA), anti-Cp33 serum recognized an antigen in the anterior end of air-dried C. parvum sporozoites but failed to bind at any sites in C. hominis sporozoites, indicating its specificity for C. parvum. IFA staining of live C. parvum sporozoites with anti-Cp33 serum failed to bind to the parasite, indicating that the CP33 antigen is not on the sporozoite surface, which is consistent with topology predictions based on the encoded amino acid sequence. RT-PCR analysis of cgd8-5370 mRNA before or during C. parvum oocyst excystation revealed transcripts only in excysting sporozoites. Thus, Cp33 represents one of a small number of proteins shown to differentiate C. parvum from C. hominis sporozoites and oocysts. C1 [Jenkins, Mark C.; O'Brien, Celia; Santin, Monica; Fayer, Ronald] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. RP Jenkins, MC (reprint author), USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. EM mark.jenkins@ars.usda.gov NR 25 TC 1 Z9 1 U1 0 U2 1 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 EI 1937-2345 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2014 VL 100 IS 4 BP 527 EP 531 DI 10.1645/13-433.1 PG 5 WC Parasitology SC Parasitology GA AO7XE UT WOS:000341565200018 PM 24601821 ER PT J AU Hoberg, EP AF Hoberg, Eric P. TI Robert Lloyd Rausch-A Life in Nature and Field Biology 1921-2012 IN MEMORIAM551 SO JOURNAL OF PARASITOLOGY LA English DT Biographical-Item ID COLLARED LEMMINGS DICROSTONYX; PARANOPLOCEPHALA SPP. CESTODA; MOLECULAR SYSTEMATICS; ECHINOCOCCUS-VOGELI; HELMINTH FAUNA; SP-N; ANOPLOCEPHALIDAE; ALASKA; PARASITE; BERINGIA C1 USDA ARS, US Natl Parasite Collect, Beltsville, MD 20705 USA. RP Hoberg, EP (reprint author), USDA ARS, US Natl Parasite Collect, BARC East 1180,10300 Baltimore Ave, Beltsville, MD 20705 USA. NR 42 TC 1 Z9 1 U1 0 U2 0 PU AMER SOC PARASITOLOGISTS PI LAWRENCE PA 810 EAST 10TH STREET, LAWRENCE, KS 66044 USA SN 0022-3395 EI 1937-2345 J9 J PARASITOL JI J. Parasitol. PD AUG PY 2014 VL 100 IS 4 BP 547 EP 552 DI 10.1645/14-561.1 PG 6 WC Parasitology SC Parasitology GA AO7XE UT WOS:000341565200022 PM 24819722 ER PT J AU Green, AR Lewis, KM Barr, JT Jones, JP Lu, FC Ralph, J Vermerris, W Sattler, SE Kang, C AF Green, Abigail R. Lewis, Kevin M. Barr, John T. Jones, Jeffrey P. Lu, Fachuang Ralph, John Vermerris, Wilfred Sattler, Scott E. Kang, ChulHee TI Determination of the Structure and Catalytic Mechanism of Sorghum bicolor Caffeic Acid O-Methyltransferase and the Structural Impact of Three brown midrib12 Mutations SO PLANT PHYSIOLOGY LA English DT Article ID BROWN-MIDRIB MUTANTS; CYTOCHROME P450-DEPENDENT MONOOXYGENASE; LIGNIN BIOSYNTHESIS; COA 3-O-METHYLTRANSFERASE; REDUCES RECALCITRANCE; NDDO APPROXIMATIONS; TRANSGENIC ALFALFA; BIOFUEL PRODUCTION; ETHANOL-PRODUCTION; DOWN-REGULATION AB Using S-adenosyl-methionine as the methyl donor, caffeic acid O-methyltransferase from sorghum (Sorghum bicolor; SbCOMT) methylates the 5-hydroxyl group of its preferred substrate, 5-hydroxyconiferaldehyde. In order to determine the mechanism of SbCOMT and understand the observed reduction in the lignin syringyl-to-guaiacyl ratio of three brown midrib12 mutants that carry COMT gene missense mutations, we determined the apo-form and S-adenosyl-methionine binary complex SbCOMT crystal structures and established the ternary complex structure with 5-hydroxyconiferaldehyde by molecular modeling. These structures revealed many features shared with monocot ryegrass (Lolium perenne) and dicot alfalfa (Medicago sativa) COMTs. SbCOMT steady-state kinetic and calorimetric data suggest a random bi-bi mechanism. Based on our structural, kinetic, and thermodynamic results, we propose that the observed reactivity hierarchy among 4,5-dihydroxy-3-methoxycinnamyl (and 3,4-dihydroxycinnamyl) aldehyde, alcohol, and acid substrates arises from the ability of the aldehyde to stabilize the anionic intermediate that results from deprotonation of the 5-hydroxyl group by histidine-267. Additionally, despite the presence of other phenylpropanoid substrates in vivo, sinapaldehyde is the preferential product, as demonstrated by its low K-m for 5-hydroxyconiferaldehyde. Unlike its acid and alcohol substrates, the aldehydes exhibit product inhibition, and we propose that this is due to nonproductive binding of the S-cis-form of the aldehydes inhibiting productive binding of the S-trans-form. The S-cis-aldehydes most likely act only as inhibitors, because the high rotational energy barrier around the 2-propenyl bond prevents S-trans-conversion, unlike alcohol substrates, whose low 2-propenyl bond rotational energy barrier enables rapid S-cis/S-trans-interconversion. C1 [Green, Abigail R.; Kang, ChulHee] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. [Lewis, Kevin M.; Barr, John T.; Jones, Jeffrey P.; Kang, ChulHee] Washington State Univ, Dept Chem, Pullman, WA 99164 USA. [Lu, Fachuang; Ralph, John] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Dept Biochem, Madison, WI 53726 USA. [Lu, Fachuang; Ralph, John] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Dept Energy, Madison, WI 53726 USA. [Vermerris, Wilfred] Univ Florida, Dept Microbiol, Gainesville, FL 32610 USA. [Vermerris, Wilfred] Univ Florida, Cell Sci & Genet Inst, Gainesville, FL 32610 USA. [Sattler, Scott E.] USDA ARS, Grain Forage & Bioenergy Res Unit, Lincoln, NE 68583 USA. RP Kang, C (reprint author), Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA. EM chkang@wsu.edu FU National Science Foundation [MCB 102114]; U.S. Department of Agriculture [35318-17454, 2011-10006-30358, 5440-21220-032-00D, 2011-67009-30026]; Department of Energy Great Lakes Bioenergy Research Center [DE-FC02-07ER64494] FX This work was supported by the National Science Foundation (grant no. MCB 102114 to C.K.), the U.S. Department of Agriculture (National Research Initiative grant no. 35318-17454 to C.K.; Biomass Research and Development Initiative Competitive grant no. 2011-10006-30358 to W.V.; and grant nos. 5440-21220-032-00D and 2011-67009-30026 to S.E.S.), and by the Department of Energy Great Lakes Bioenergy Research Center (grant no. DE-FC02-07ER64494 to J.R. and F.L.). NR 61 TC 8 Z9 9 U1 3 U2 23 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD AUG PY 2014 VL 165 IS 4 BP 1440 EP 1456 DI 10.1104/pp.114.241729 PG 17 WC Plant Sciences SC Plant Sciences GA AO9AT UT WOS:000341648600006 PM 24948836 ER PT J AU Stappen, I Wanner, J Tabanca, N Wedge, DE Ali, A Khan, IA Kaul, VK Lal, B Jaitak, V Gochev, V Girova, T Stoyanova, A Schmidt, E Jirovetz, L AF Stappen, Iris Wanner, Juergen Tabanca, Nurhayat Wedge, David E. Ali, Abbas Khan, Ikhlas A. Kaul, Vijay K. Lal, Brij Jaitak, Vikas Gochev, Velizar Girova, Tania Stoyanova, Albena Schmidt, Erich Jirovetz, Leopold TI Chemical Composition and Biological Effects of Artemisia maritima and Artemisia nilagirica Essential Oils from Wild Plants of Western Himalaya SO PLANTA MEDICA LA English DT Article DE Artemisia nilagirica; Artemisia maritima; Asteraceae; antifungal activity; antibacterial activity; biting deterrence; larvicidal activity ID AEDES-AEGYPTI DIPTERA; ANTIMICROBIAL ACTIVITY; VOLATILE CONSTITUENTS; VAR. SEPTENTRIONALIS; ANTIFUNGAL ACTIVITY; CULICIDAE; DISCOVERY; PAKISTAN; AREAS; INDIA AB Artemisia species possess pharmacological properties that are used for medical purposes worldwide. In this paper, the essential oils from the aerial parts of Artemisia nilagirica and Artemisia maritima from the western Indian Himalaya region are described. The main compounds analyzed by simultaneous GC/MS and GC/FID were camphor and 1,8-cineole from A. maritima, and camphor and artemisia ketone from A. nilagirica. Additionally, the oils were evaluated for their antibacterial, antifungal, mosquito biting deterrent, and larvicidal activities. A. nilagirica essential oil demonstrated nonselective antifungal activity against plant pathogens Colletotrichum acutatum, Colletotrichum fragariae, and Colletotrichum gloeosporioides, whereas A. maritima did not show antifungal activity. Both Artemisia spp. exhibited considerable mosquito biting deterrence, whereas only A. nilagirica showed larvicidal activity against Aedes aegypti. Antibacterial effects assessed by an agar dilution assay demonstrated greater activity of A. maritima essential oil against Staphylococcus aureus and Pseudomonas aeruginosa compared to A. nilagirica. C1 [Stappen, Iris; Schmidt, Erich; Jirovetz, Leopold] Univ Vienna, Dept Pharmaceut Chem, A-1090 Vienna, Austria. [Wanner, Juergen] Kurt Kitzing Co, Wallerstein, Germany. [Tabanca, Nurhayat; Ali, Abbas; Khan, Ikhlas A.] Univ Mississippi, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Wedge, David E.] Univ Mississippi, USDA, ARS, Nat Prod Utilizat Res Unit, University, MS 38677 USA. [Khan, Ikhlas A.] Univ Mississippi, Sch Pharm, Dept Pharmacognosy, University, MS 38677 USA. [Kaul, Vijay K.] CSIR Inst Himalayan Bioresource Technol, Palampur, Himachal Prades, India. [Lal, Brij; Jaitak, Vikas] Inst Himalayan Bioresource Technol, Biodivers Div, Palampur, Himachal Prades, India. [Jaitak, Vikas] Cent Univ Punjab, Ctr Chem & Pharmaceut Sci, Bathinda, India. [Gochev, Velizar; Girova, Tania] Paisii Hilendarski Univ Plovdiv, Dept Biochem & Microbiol, Plovdiv, Bulgaria. [Stoyanova, Albena] Univ Food Technol, Dept Essential Oils, Plovdiv, Bulgaria. RP Stappen, I (reprint author), Univ Vienna, Dept Pharmaceut Chem, Althanstr 14, A-1090 Vienna, Austria. EM iris.stappen@univie.ac.at FU USDA/ARS [56-6402-1-612]; Deployed War-Fighter Protection research program; U.S. Department of Defense through the Armed Forces Pest Management Board FX This study was supported, in part, by USDA/ARS grant No. 56-6402-1-612 and the Deployed War-Fighter Protection research program grant funded by the U.S. Department of Defense through the Armed Forces Pest Management Board. We thank Ms. J. L. Robertson and Ms. R. Pace for their help with the antifungal bioassays and Dr. James J. Becnel, Mosquito and Fly Research Unit, Center for Medical, Agricultural and Veterinary Entomology, USDA-ARS, Gainesville, for supplying Ae. aegypti eggs. Vijay K. Kaul, Brij Lal and Vikas Jaitak are grateful to Director CSIR-IHBT, Palampur, for providing necessary facilities for carrying out surveys, plant collection, necessary extractions and analytical work. NR 49 TC 4 Z9 4 U1 2 U2 22 PU GEORG THIEME VERLAG KG PI STUTTGART PA RUDIGERSTR 14, D-70469 STUTTGART, GERMANY SN 0032-0943 EI 1439-0221 J9 PLANTA MED JI Planta Med. PD AUG PY 2014 VL 80 IS 13 BP 1079 EP 1087 DI 10.1055/s-0034-1382957 PG 9 WC Plant Sciences; Chemistry, Medicinal; Integrative & Complementary Medicine; Pharmacology & Pharmacy SC Plant Sciences; Pharmacology & Pharmacy; Integrative & Complementary Medicine GA AO8CX UT WOS:000341581600004 PM 25127023 ER PT J AU Olukolu, BA Wang, GF Vontimitta, V Venkata, BP Marla, S Ji, JB Gachomo, E Chu, K Negeri, A Benson, J Nelson, R Bradbury, P Nielsen, D Holland, JB Balint-Kurti, PJ Johal, G AF Olukolu, Bode A. Wang, Guan-Feng Vontimitta, Vijay Venkata, Bala P. Marla, Sandeep Ji, Jiabing Gachomo, Emma Chu, Kevin Negeri, Adisu Benson, Jacqueline Nelson, Rebecca Bradbury, Peter Nielsen, Dahlia Holland, James B. Balint-Kurti, Peter J. Johal, Gurmukh TI A Genome-Wide Association Study of the Maize Hypersensitive Defense Response Identifies Genes That Cluster in Related Pathways SO PLOS GENETICS LA English DT Article ID PROGRAMMED CELL-DEATH; PLANT-DISEASE RESISTANCE; LESION MIMIC MUTANTS; MAPPING POPULATION; FUNGAL PATHOGENS; RUST RESISTANCE; QUANTITATIVE RESISTANCE; NECROTROPHIC PATHOGENS; OXIDATIVE BURST; IMMUNE-RESPONSE AB Much remains unknown of molecular events controlling the plant hypersensitive defense response (HR), a rapid localized cell death that limits pathogen spread and is mediated by resistance (R-) genes. Genetic control of the HR is hard to quantify due to its microscopic and rapid nature. Natural modifiers of the ectopic HR phenotype induced by an aberrant auto-active R-gene (Rp1-D21), were mapped in a population of 3,381 recombinant inbred lines from the maize nested association mapping population. Joint linkage analysis was conducted to identify 32 additive but no epistatic quantitative trait loci (QTL) using a linkage map based on more than 7000 single nucleotide polymorphisms (SNPs). Genome-wide association (GWA) analysis of 26.5 million SNPs was conducted after adjusting for background QTL. GWA identified associated SNPs that colocalized with 44 candidate genes. Thirty-six of these genes colocalized within 23 of the 32 QTL identified by joint linkage analysis. The candidate genes included genes predicted to be in involved programmed cell death, defense response, ubiquitination, redox homeostasis, autophagy, calcium signalling, lignin biosynthesis and cell wall modification. Twelve of the candidate genes showed significant differential expression between isogenic lines differing for the presence of Rp1-D21. Low but significant correlations between HR-related traits and several previously-measured disease resistance traits suggested that the genetic control of these traits was substantially, though not entirely, independent. This study provides the first system-wide analysis of natural variation that modulates the HR response in plants. C1 [Olukolu, Bode A.; Wang, Guan-Feng; Negeri, Adisu; Balint-Kurti, Peter J.] NC State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Vontimitta, Vijay; Venkata, Bala P.; Marla, Sandeep; Ji, Jiabing; Gachomo, Emma; Chu, Kevin; Johal, Gurmukh] Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA. [Benson, Jacqueline; Nelson, Rebecca] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Bradbury, Peter] Cornell Univ, Inst Genom Divers, Ithaca, NY USA. [Nielsen, Dahlia] NC State Univ, Dept Biol Sci, Raleigh, NC USA. [Holland, James B.; Balint-Kurti, Peter J.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Holland, James B.] NC State Univ, Dept Crop Sci, Raleigh, NC USA. RP Olukolu, BA (reprint author), NC State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. EM peter.balint-kurti@ars.usda.gov OI Balint-Kurti, Peter/0000-0002-3916-194X FU USDA-ARS; Purdue University; National Science Foundation [0822495, IOS-0820619] FX This work was funded by USDA-ARS, Purdue University and National Science Foundation grants 0822495 and IOS-0820619. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 111 TC 7 Z9 7 U1 8 U2 47 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1553-7390 EI 1553-7404 J9 PLOS GENET JI PLoS Genet. PD AUG PY 2014 VL 10 IS 8 AR e1004562 DI 10.1371/journal.pgen.1004562 PG 15 WC Genetics & Heredity SC Genetics & Heredity GA AO8BQ UT WOS:000341577800048 PM 25166276 ER PT J AU Slininger, PJ Dien, BS Lomont, JM Bothast, RJ Ladisch, MR Okos, MR AF Slininger, P. J. Dien, B. S. Lomont, J. M. Bothast, R. J. Ladisch, M. R. Okos, M. R. TI Evaluation of a Kinetic Model for Computer Simulation of Growth and Fermentation by Scheffersomyces (Pichia) stipitis Fed D-Xylose SO BIOTECHNOLOGY AND BIOENGINEERING LA English DT Article DE lignocellulose; ethanol; model; simulation; fed-batch; cell recycle; ethanol stripping ID ETHANOL-PRODUCTION; FERMENTING YEAST; NRRL Y-7124; PACHYSOLEN-TANNOPHILUS; CONVERSION; PHYSIOLOGY; AERATION; PH AB Scheffersomyces (formerly Pichia) stipitis is a potential biocatalyst for converting lignocelluloses to ethanol because the yeast natively ferments xylose. An unstructured kinetic model based upon a system of linear differential equations has been formulated that describes growth and ethanol production as functions of ethanol, oxygen, and xylose concentrations for both growth and fermentation stages. The model was validated for various growth conditions including batch, cell recycle, batch with in situ ethanol removal and fed-batch. The model provides a summary of basic physiological yeast properties and is an important tool for simulating and optimizing various culture conditions and evaluating various bioreactor designs for ethanol production. (C) 2014 Wiley Periodicals, Inc. C1 [Slininger, P. J.; Dien, B. S.; Lomont, J. M.; Bothast, R. J.] ARS, USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. [Lomont, J. M.; Ladisch, M. R.; Okos, M. R.] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA. RP Slininger, PJ (reprint author), ARS, USDA, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. EM pat.slininger@ars.usda.gov OI Dien, Bruce/0000-0003-3863-6664 NR 36 TC 5 Z9 5 U1 0 U2 21 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0006-3592 EI 1097-0290 J9 BIOTECHNOL BIOENG JI Biotechnol. Bioeng. PD AUG PY 2014 VL 111 IS 8 BP 1532 EP 1540 DI 10.1002/bit.25215 PG 9 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AO3MS UT WOS:000341235400007 PM 24519334 ER PT J AU Hart, JL Oswalt, CM Turberville, CM AF Hart, Justin L. Oswalt, Christopher M. Turberville, Craig M. TI Population dynamics of sugar maple through the southern portion of its range: implications for range migration SO BOTANY-BOTANIQUE LA English DT Article DE climate change; range migration; succession; sugar maple (Acer saccharum) ID EASTERN UNITED-STATES; CUMBERLAND PLATEAU; CLIMATE-CHANGE; HARDWOOD FOREST; ACER-SACCHARUM; RED MAPLE; GROWTH; PATTERNS; SHIFTS; CHALLENGES AB The range of sugar maple (Acer saccharum Marsh.) is expected to shift northward in accord with changing climate. However, a pattern of increased sugar maple abundance has been reported from sites throughout the eastern US. The goal of our study was to examine the stability of the sugar maple southern range boundary by analyzing its demography through the southern extent of its distribution. We analyzed changes in sugar maple basal area, relative frequency, relative density, relative importance values, diameter distributions, and the ratio of sapling biomass to total sugar maple biomass at three spatial positions near the southern boundary of the species' range using forest inventory data from the USDA Forest Service Forest Inventory and Analysis program over a 20 year observation period (1990-2010). We contend that the southern range boundary of sugar maple neither contracted nor expanded during this period. We speculated that biophysical changes caused by succession may provide a short-lived ameliorative barrier to a rapid southern range contraction for some species. The eclipse of some greater climate change threshold may therefore be required to realize significant range movement for mesophytic tree species. C1 [Hart, Justin L.; Turberville, Craig M.] Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. [Oswalt, Christopher M.] US Forest Serv, Southern Res Stn, USDA, Knoxville, TN 37917 USA. RP Hart, JL (reprint author), Univ Alabama, Dept Geog, Box 870322, Tuscaloosa, AL 35487 USA. EM hart013@ua.edu RI Hart, Justin/B-2535-2010 OI Hart, Justin/0000-0001-6580-0189 NR 47 TC 1 Z9 1 U1 5 U2 29 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 1916-2790 EI 1916-2804 J9 BOTANY JI Botany PD AUG PY 2014 VL 92 IS 8 BP 563 EP 569 DI 10.1139/cjb-2014-0008 PG 7 WC Plant Sciences SC Plant Sciences GA AO7FR UT WOS:000341518300003 ER PT J AU Railsback, SF Harvey, BC White, JL AF Railsback, Steven F. Harvey, Bret C. White, Jason L. TI Facultative anadromy in salmonids: linking habitat, individual life history decisions, and population-level consequences SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID CALIFORNIA CENTRAL COAST; ONCORHYNCHUS-MYKISS; ATLANTIC SALMON; CENTRAL VALLEY; PATTERNS; STEELHEAD; RESIDENT; BEHAVIOR; MODELS; TROUT AB Modeling and management of facultative anadromous salmonids is complicated by their ability to select anadromous or resident life histories. Conventional theory for this behavior assumes individuals select the strategy offering highest expected reproductive success but does not predict how population-level consequences such as a stream's smolt production emerge from the anadromy decision and habitat conditions. Our individual-based population model represents juvenile growth, survival, and anadromy decisions as outcomes of habitat and competition. In simulation experiments that varied stream growth and survival conditions, we examined how many simulated juveniles selected anadromy versus residence and how many of those choosing anadromy survived until smolting. Owing to variation in habitat and among individuals, the within-population frequency of anadromy changed gradually with growth and survival conditions instead of switching abruptly. Higher predation risk caused more juveniles to select anadromy, but fewer survived long enough to smolt. Improving growth appears a much safer way to increase smolt production compared with reducing freshwater survival. Smolt production peaked at high growth and moderately high survival, conditions that also produced many residents. C1 [Railsback, Steven F.] Lang Railsback & Associates, Arcata, CA 95521 USA. [Harvey, Bret C.; White, Jason L.] US Forest Serv, USDA, Pacific Southwest Res Stn, Arcata, CA 95521 USA. RP Railsback, SF (reprint author), Lang Railsback & Associates, 250 Calif Ave, Arcata, CA 95521 USA. EM Steve@LangRailsback.com OI Railsback, Steven/0000-0002-5923-9847 FU Bay-Delta office of the US Fish and Wildlife Service FX Development of the inSALMO-FA model was funded by the Bay-Delta office of the US Fish and Wildlife Service, Julie Zimmerman, project manager. Habitat input and hydraulic simulations were provided by Mark Gard and fisheries data by Matt Brown, James Earley, and Sarah Gallagher, all US Fish and Wildlife Service. Colin Sheppard was the software developer. We thank Will Satterthwaite for advice in developing the model and Jason Dunham for much very helpful input. NR 35 TC 4 Z9 3 U1 3 U2 38 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD AUG PY 2014 VL 71 IS 8 BP 1270 EP 1278 DI 10.1139/cjfas-2014-0091 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AO7FN UT WOS:000341517800013 ER PT J AU Stevens, JT Safford, HD Latimer, AM AF Stevens, Jens T. Safford, Hugh D. Latimer, Andrew M. TI Wildfire-contingent effects of fuel treatments can promote ecological resilience in seasonally dry conifer forests SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article DE California; forests; fuel treatments; mixed conifer; resilience; restoration; yellow pine; wildfire ID PONDEROSA PINE FORESTS; WESTERN UNITED-STATES; FIRE SEVERITY; REDUCTION TREATMENTS; SIERRA-NEVADA; RESTORATION TREATMENTS; VEGETATION STRUCTURE; CALIFORNIA; USA; EMISSIONS AB Fire suppression has made many seasonally dry conifer forests more susceptible to high-severity wildfires, which cause large changes in forest structure and function. In response, management agencies are applying fuel reduction treatments to millions of acres of forest, with the goal of moderating fire behavior by reducing tree density and understory fuel loads. However, despite their wide application, we still lack basic information about the extent to which these treatments contribute to forest restoration by increasing forest resilience to recurring wildfire events. To address this question, we established 664 plots across 12 different sites in California, USA, where wildfire burned through fuel treatments, and measured a suite of forest characteristics relating to overstory structure, understory cover, and woody plant regeneration. We tested a "wildfire-contingency" hypothesis that there should be strong interactions between treatment and fire, specifically that the direction and magnitude of fuel treatment effects on forest characteristics will depend on subsequent disturbance. This interaction hypothesis had strong support, driven largely by effects on trees: without wildfire, live-tree cover was lower in treated stands than in untreated stands, but after wildfire, it was higher in treated stands than in untreated stands. Treated stands had higher soil moisture and more shrub seedlings than untreated stands without wildfire but had greater soil moisture and fewer shrub seedlings than untreated stands after wildfire. Conversely, litter depth, litter cover, and tree seedling abundance were lower in treated stands than in untreated stands without wildfire but higher in treated stands than in untreated stands after wildfire. Ordination revealed that the magnitude of ecological change attributable to wildfire is lower in treated stands than in untreated stands. We conclude that properly implemented treatments can promote resilience to both first-entry and subsequent wildfires. C1 [Stevens, Jens T.] Univ Calif Davis, Grad Grp Ecol, Davis, CA 95616 USA. [Stevens, Jens T.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Safford, Hugh D.] US Forest Serv, USDA, Vallejo, CA 94592 USA. [Safford, Hugh D.] Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA. [Latimer, Andrew M.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. RP Stevens, JT (reprint author), Univ Calif Davis, Grad Grp Ecol, Davis, CA 95616 USA. EM jtstevens@ucdavis.edu OI Stevens, Jens/0000-0002-2234-1960 FU USDA Forest Service, Pacific Southwest Region; California Energy Commission FX We thank Amy Jirka, Sean Glantz, Kevin Duggan, Taylor Farnum, Angelica Sauceda, Sveta Yegorova, Susan Magnoli, Karina Silvas-Bellanca, Dave Schmidt, and Marc Meyer for assistance with fieldwork. Staff at the following National Forests assisted with locating our sampling plots: Modoc, Lassen, Plumas, Tahoe, Lake Tahoe Basin, Sierra, Sequoia, and San Bernardino. We thank Susan Harrison and three anonymous reviewers for helpful comments on this manuscript. Our work was funded by the USDA Forest Service, Pacific Southwest Region, and the California Energy Commission. NR 67 TC 16 Z9 16 U1 4 U2 47 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD AUG PY 2014 VL 44 IS 8 BP 843 EP 854 DI 10.1139/cjfr-2013-0460 PG 12 WC Forestry SC Forestry GA AO7FH UT WOS:000341517100001 ER PT J AU Slesak, RA Lenhart, CF Brooks, KN D'Amato, AW Palik, BJ AF Slesak, Robert A. Lenhart, Christian F. Brooks, Kenneth N. D'Amato, Anthony W. Palik, Brian J. TI Water table response to harvesting and simulated emerald ash borer mortality in black ash wetlands in Minnesota, USA SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article DE water table rise; forested wetlands; invasive species; Fraxinus nigra Marshall; forest management; Agrilus planipennis Fairmaire ID FORESTED WETLANDS; DYNAMICS; TREES; DEPTH AB Black ash wetlands are seriously threatened because of the invasive emerald ash borer (EAB). Wetland hydrology is likely to be modified following ash mortality, but the magnitude of hydrological impact following loss via EAB and alternative mitigation harvests is not clear. Our objective was to assess the water table response to simulated EAB and harvesting to determine if management actions will be needed to maintain ecosystem functions following EAB infestation. We applied four replicated treatments to 1.6 ha plots as follows: (1) control, (2) girdling of all black ash trees to simulate loss via EAB mortality, (3) group selection harvests (20% of stand in 0.04 ha gaps), and (4) clear-cut harvest. Water table (WT) elevations were monitored for 1 year pre-treatment and two years post-treatment. Clear-cutting delayed WT drawdown in both years of the study, and the WT was significantly higher than the control treatment, predominantly when WT depth was below 30 cm. The effect of the group selection treatment on WT response was muted compared to clear-cutting and also limited to periods when the WT depth was below 30 cm. These responses were attributed to establishment of shallo-wrooted vegetation in cut areas, which would have limited influence on WT dynamics as depth increased. There was little effect of girdling on the WT in the first year post-treatment, but effects on the WT were very similar to clear-cutting in the second year and more pronounced when the WT was within 30 cm of the soil surface. These effects were attributed to reduced transpiration coupled with the presence of a partial canopy following girdling, which would have reduced vegetation establishment and evaporation compared to clear-cutting. Given the large influence of WT depth on vegetation dynamics and associated feedbacks to altered hydrology, these early results indicate a greater risk of ecosystem alteration following EAB mortality compared to clear-cut harvesting. Depending on local hydrologic regime, variation in precipitation patterns, and time for complete canopy loss, it may be necessary for managers to implement active mitigation strategies (e. g., group selection coupled with planting of alternative species) prior to EAB infestation to maintain ecosystem processes in these forested wetland systems. C1 [Slesak, Robert A.] Minnesota Forest Resources Council, St Paul, MN 55108 USA. [Lenhart, Christian F.] Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA. [Brooks, Kenneth N.; D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Palik, Brian J.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. RP Slesak, RA (reprint author), Minnesota Forest Resources Council, 115 Green Hall,1530 Cleveland Ave N, St Paul, MN 55108 USA. EM raslesak@umn.edu FU Minnesota Environmental and Natural Resources Trust Fund; USDA Forest Service Northern Research Station; Minnesota Forest Resources Council; Minnesota Agricultural Experiment Station FX This project was funded by the Minnesota Environmental and Natural Resources Trust Fund, the USDA Forest Service Northern Research Station, the Minnesota Forest Resources Council, and the Minnesota Agricultural Experiment Station. We gratefully acknowledge the assistance of Gary Swanson and other Chippewa National Forest staff with project development and support. Assistance with field work and data collection was provided by Doug Kastendick, John Elioff, Joe Shannon, Mitch Slater, Mark Davidson, Caleb Arika, and Al Waller. NR 31 TC 9 Z9 9 U1 10 U2 50 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD AUG PY 2014 VL 44 IS 8 BP 961 EP 968 DI 10.1139/cjfr-2014-0111 PG 8 WC Forestry SC Forestry GA AO7FH UT WOS:000341517100011 ER PT J AU Brockway, DG Loewenstein, EF Outcalt, KW AF Brockway, Dale G. Loewenstein, Edward F. Outcalt, Kenneth W. TI Proportional basal area method for implementing selection silviculture systems in longleaf pine forests SO CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE LA English DT Article DE Pinus palustris Mill.; multiaged silviculture; single-tree selection; group selection; Proportional-B ID UNEVEN-AGED SILVICULTURE; NATURAL DISTURBANCE; MULTIAGED STANDS; STOCKING CONTROL; ECOSYSTEM; REGENERATION; MANAGEMENT; FIRE; OBJECTIVES; RESOURCES AB Proportional basal area (Pro-B) was developed as an accurate, easy-to-use method for making uneven-aged silviculture a practical management option. Following less than 3 h of training, forest staff from a range of professional backgrounds used Pro-B in an operational-scale field study to apply single-tree selection and group selection systems in longleaf pine (Pinus palustris Mill.) stands. Field crews achieved precision levels often within 3%-5% of the 11.5 m(2) ha(-1) target residual basal area. By aggregating many diameter classes into only three diameter-class groups, Pro-B improves efficiency by requiring tree markers to remember only three fractions, while making a single pass through the stand. Trees of large size, specific species and with good form, broad crowns and cavities can be retained, while adjusting spacing to release residuals. Systematic quantification of marking trees for removal enables different individuals to obtain similar results. Early observations revealed encouraging levels of pine regeneration and stand development, along with continuing good volume growth rates of 3% per year. Although less certain until one or more cutting cycles are completed, these early tests indicate that a stable mature forest structure should develop, which is characterized by the presence of large trees and natural regeneration. C1 [Brockway, Dale G.] US Forest Serv, Southern Res Stn, USDA, Auburn, AL 36849 USA. [Loewenstein, Edward F.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. [Outcalt, Kenneth W.] US Forest Serv, Southern Res Stn, USDA, Athens, GA 30602 USA. RP Brockway, DG (reprint author), US Forest Serv, Southern Res Stn, USDA, 521 Devall Dr, Auburn, AL 36849 USA. EM dbrockway@fs.fed.us FU Florida Division of Forestry [SRS-04-CO-11330123-043] FX Special thanks to Jeremy Waites, Matt Reilly, David Combs, David Jones, Bryan Bulger, Ron Tucker, Chris Colburn, Andy Lamborn, Eric Neiswanger, Jason O'Shell, Mike Allen, and Tom Phillips for field assistance and to Laura Kenefic, Chris Oswalt and two anonymous reviewers for comments on this manuscript. This work was supported by the Florida Division of Forestry [agreement SRS-04-CO-11330123-043]. NR 56 TC 5 Z9 5 U1 1 U2 15 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res.-Rev. Can. Rech. For. PD AUG PY 2014 VL 44 IS 8 BP 977 EP 985 DI 10.1139/cjfr-2013-0510 PG 9 WC Forestry SC Forestry GA AO7FH UT WOS:000341517100013 ER PT J AU Wu, XW Moon, RJ Martini, A AF Wu, Xiawa Moon, Robert J. Martini, Ashlie TI Tensile strength of I beta crystalline cellulose predicted by molecular dynamics simulation SO CELLULOSE LA English DT Article DE Cellulose; Tensile strength; Failure Elastic modulus; Poisson's ratio; Molecular dynamics ID ELASTIC-MODULUS; X-RAY; DEFORMATION; NANOCRYSTALS; TEMPERATURE; NANOFIBRILS; NANOWIRES; MECHANICS AB The mechanical properties of Ib crystalline cellulose are studied using molecular dynamics simulation. A model Ib crystal is deformed in the three orthogonal directions at three different strain rates. The stress-strain behaviors for each case are analyzed and then used to calculate mechanical properties. The results show that the elastic modulus, Poisson's ratio, yield stress and strain, and ultimate stress and strain are highly anisotropic. In addition, while the properties that describe the elastic behavior of the material are independent of strain rate, the yield and ultimate properties increase with increasing strain rate. The deformation and failure modes associated with these properties and the relationships between the material's response to tension and the evolution of the crystal structure are analyzed. C1 [Wu, Xiawa] Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA. [Moon, Robert J.] US Forest Serv, Forest Prod Lab, Madison, WI 53705 USA. [Martini, Ashlie] Univ Calif Merced, Sch Engn, Merced, CA USA. RP Wu, XW (reprint author), Purdue Univ, Sch Mech Engn, W Lafayette, IN 47907 USA. EM wu123@purdue.edu; robertmoon@fs.fed.us; amartini@ucmerced.edu FU Air Force Office [FA9550-11-1-0162] FX The authors thank the Air Force Office of Sponsored Research Grant: FA9550-11-1-0162 for support of this research. NR 40 TC 9 Z9 9 U1 1 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD AUG PY 2014 VL 21 IS 4 BP 2233 EP 2245 PG 13 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AO6VM UT WOS:000341490200005 ER PT J AU Rosenau, T Potthast, A Krainz, K Hettegger, H Henniges, U Yoneda, Y Rohrer, C French, AD AF Rosenau, Thomas Potthast, Antje Krainz, Karin Hettegger, Hubert Henniges, Ute Yoneda, Yuko Rohrer, Christian French, Alfred D. TI Chromophores in cellulosics, XI: isolation and identification of residual chromophores from bacterial cellulose SO CELLULOSE LA English DT Article DE Bacterial cellulose; Chromophores; Aging; Bleaching; CRI method; Hydroxybenzoquinones; Hydroxynaphthoquinones; Hydroxyacetophenones; Tryptophan ID INDUCED BOND LOCALIZATION; N-OXIDE LYOCELL; AROMATIC-COMPOUNDS; AQUEOUS-SOLUTION; CARBONYL GROUPS; AUXIN BIOSYNTHESIS; AGED CELLULOSICS; KEY CHROMOPHORE; D-GLUCOSE; DISCOLORATION AB In the present work, bacterial cellulose (BC) was analyzed for its chromophore content with the chromophore release and identification (CRI) method. In aged BC, seven chromophores were unambiguously identified, despite their very low (ppb) presence. The compounds contain 2-hydroxy[ 1,4] benzoquinone, 2-hydroxyacetophenone and 5,8dihydroxy-naphthoquinone moieties. These three classes of key chromophores had also been found in other celluloses such as bleached pulp, fiber and derivatives. The detection of chromophores in ligninfree BC confirms that the chromophores are formed from oxidized and degraded carbohydrate material rather than lignin fragments. Samples that were insufficiently purified before the aging step also yielded three N-heterocyclic compounds. They originated from tryptophan moieties in residual adhering protein and protein degradation products. NHeteropentacycles 9 and 10 showed strong adsorption onto cellulosic surfaces, and that adsorption aided oxidation of cyclohexadiene derivative 9 into the corresponding aromatic and intensely yellow system 10, consistent with the strain-induced bond localization theory. In purified fresh BC, no chromophores were detectable by the CRI method. The present contribution is the first report on defined chromophoric structures isolated from BC. C1 [Rosenau, Thomas; Potthast, Antje; Krainz, Karin; Hettegger, Hubert; Henniges, Ute; Yoneda, Yuko] Univ Nat Resources & Life Sci Vienna BOKU, Dept Chem, Div Chem Renewables, A-1190 Vienna, Austria. [Rohrer, Christian] Lohmann & Rauscher GmbH & Co KG, R&D Dept, D-56567 Neuwied, Germany. [French, Alfred D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Rosenau, T (reprint author), Univ Nat Resources & Life Sci Vienna BOKU, Dept Chem, Div Chem Renewables, Muthgasse 18, A-1190 Vienna, Austria. EM thomas.rosenau@boku.ac.at FU Austrian Christian-Doppler-Society through "Christian Doppler Laboratory for Advanced Cellulose Chemistry and Analytics" FX We would like to thank the Austrian Christian-Doppler-Society for financial support through the "Christian Doppler Laboratory for Advanced Cellulose Chemistry and Analytics". Prof. Dieter Klemm, Jena University, Germany, as well as the FZMB Bad Langensalza are acknowledged for the gift of differently prepared BC samples. NR 39 TC 7 Z9 7 U1 1 U2 17 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD AUG PY 2014 VL 21 IS 4 BP 2271 EP 2283 DI 10.1007/s10570-014-0289-0 PG 13 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AO6VM UT WOS:000341490200008 ER PT J AU Nam, S Condon, BD AF Nam, Sunghyun Condon, Brian D. TI Internally dispersed synthesis of uniform silver nanoparticles via in situ reduction of [Ag(NH3)(2)](+) along natural microfibrillar substructures of cotton fiber SO CELLULOSE LA English DT Article DE Cotton; Silver nanoparticle; Self-controlled microfibrillar reactor; Nanofluidic system; Nanocomposite fiber ID GOLD NANOPARTICLES; ANTIMICROBIAL ACTIVITY; METAL NANOPARTICLES; ESCHERICHIA-COLI; CRYSTALLITE SIZE; SEGMENTED FLOW; CELLULOSE-II; FABRICS; TOXICITY; RELEASE AB Silver nanoparticles (Ag NPs) are known to have efficient antimicrobial properties, but the direct application of Ag NPs onto the surface of textiles has shown to be ineffective and raise environmental concerns because Ag NPs leach out during washing. In this study, non-leaching and stable Ag-cotton nanocomposite fiber was produced by the in situ formation of Ag NPs inside the cotton fiber. The reported method is to introduce a nanofluidic system in alkali-swollen cotton fiber. Sequential flows of [Ag(NH3)(2)](+) and reductant aqueous fluids into the opened microfibrillar channels yielded a self-assembly of Ag ions on the deprotonated cellulose and subsequent nucleation and particle growth on the microfibrils. Transmission electron and field emission scanning electron microscopy images showed Ag NPs evenly dispersed throughout the entire cross-section of the fiber and their fixation onto the isolated secondary cell wall, respectively. Despite the rapid reduction reaction and the absence of a stabilizing agent, the successful formation of monodispersed Ag NPs (12 +/- 3 nm) was attributed to the self-controlled function of the highly organized microfibrillar substructures, which regulated the transport and mixing of reactants. Incorporation of Ag NPs into the internal structure of the cotton fiber did not significantly influence the cotton crystalline structure. C1 [Nam, Sunghyun; Condon, Brian D.] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Condon, BD (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM brian.condon@ars.usda.gov NR 56 TC 7 Z9 7 U1 1 U2 21 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0969-0239 EI 1572-882X J9 CELLULOSE JI Cellulose PD AUG PY 2014 VL 21 IS 4 BP 2963 EP 2972 DI 10.1007/s10570-014-0270-y PG 10 WC Materials Science, Paper & Wood; Materials Science, Textiles; Polymer Science SC Materials Science; Polymer Science GA AO6VM UT WOS:000341490200064 ER PT J AU Chang, CF Schock, EN O'Hare, EA Dodgson, J Cheng, HH Muir, WM Edelmann, RE Delany, ME Brugmann, SA AF Chang, Ching-Fang Schock, Elizabeth N. O'Hare, Elizabeth A. Dodgson, Jerry Cheng, Hans H. Muir, William M. Edelmann, Richard E. Delany, Mary E. Brugmann, Samantha A. TI The cellular and molecular etiology of the craniofacial defects in the avian ciliopathic mutant SO DEVELOPMENT LA English DT Article DE Primary cilia; Craniofacial; talpid; Gli processing; Hedgehog signaling; Ciliopathies; Chicken ID GREIG CEPHALOPOLYSYNDACTYLY SYNDROME; PRIMARY CILIA; DEVELOPMENTAL ABNORMALITIES; REPRESSOR FUNCTIONS; CHICKEN EMBRYOS; TALPID3 MUTANT; GLI PROTEINS; EXTRA-TOES; GENE; MOUSE AB talpid(2) is an avian autosomal recessive mutant with a myriad of congenital malformations, including polydactyly and facial clefting. Although phenotypically similar to talpid(3), talpid(2) has a distinct facial phenotype and an unknown cellular, molecular and genetic basis. We set out to determine the etiology of the craniofacial phenotype of this mutant. We confirmed that primary cilia were disrupted in talpid(2) mutants. Molecularly, we found disruptions in Hedgehog signaling. Posttranslational processing of GLI2 and GLI3 was aberrant in the developing facial prominences. Although both GLI2 and GLI3 processing were disrupted in talpid(2) mutants, only GLI3 activator levels were significantly altered in the nucleus. Through additional fine mapping and whole-genome sequencing, we determined that the talpid(2) phenotype was linked to a 1.4 Mb region on GGA1q that contained the gene encoding the ciliary protein C2CD3. We cloned the avian ortholog of C2CD3 and found its expression was ubiquitous, but most robust in the developing limbs and facial prominences. Furthermore, we found that C2CD3 is localized proximal to the ciliary axoneme and is important for docking the mother centriole to the ciliary vesicle and cell membrane. Finally, we identified a 19 bp deletion in talpid(2) C2CD3 that produces a premature stop codon, and thus a truncated protein, as the likely causal allele for the phenotype. Together, these data provide insight into the cellular, molecular and genetic etiology of the talpid(2) phenotype. Our data suggest that, although the talpid(2) and talpid(3) mutations affect a common ciliogenesis pathway, they are caused by mutations in different ciliary proteins that result in differences in craniofacial phenotype. C1 [Chang, Ching-Fang; Schock, Elizabeth N.; Brugmann, Samantha A.] Cincinnati Childrens Hosp Med Ctr, Dept Surg, Div Plast Surg, Cincinnati, OH 45229 USA. [Chang, Ching-Fang; Schock, Elizabeth N.; Brugmann, Samantha A.] Cincinnati Childrens Hosp Med Ctr, Dept Pediat, Div Dev Biol, Cincinnati, OH 45229 USA. [O'Hare, Elizabeth A.; Delany, Mary E.] Univ Calif Davis, Coll Agr & Environm Sci, Dept Anim Sci, Davis, CA 95616 USA. [Dodgson, Jerry] Michigan State Univ, E Lansing, MI 48824 USA. [Cheng, Hans H.] USDA, Avian Dis & Oncol Lab, E Lansing, MI 48823 USA. [Muir, William M.] Purdue Univ, Dept Anim Sci, W Lafayette, IN 47907 USA. [Edelmann, Richard E.] Miami Univ, Ctr Adv Microscopy & Imaging, Oxford, OH 45056 USA. RP Brugmann, SA (reprint author), Cincinnati Childrens Hosp Med Ctr, Dept Surg, Div Plast Surg, Cincinnati, OH 45229 USA. EM samantha.brugmann@cchmc.org FU National Institutes of Health; National Institute of Dental and Craniofacial Research (NIDCR) [R00-DE01985]; Cincinnati Children's Research Foundation; John and Joan Fiddyment Endowment (University of California, Davis); NRSP-8 National Animal Genome Research Support Program [CA-D*-ASC-7233-RR] FX This work was funded by the National Institutes of Health, National Institute of Dental and Craniofacial Research (NIDCR) [R00-DE01985 to S.A.B.], by C.T.O. funds from the Cincinnati Children's Research Foundation to S.A.B., by the John and Joan Fiddyment Endowment (University of California, Davis) to M.E.D., and by the NRSP-8 National Animal Genome Research Support Program [CA-D*-ASC-7233-RR to M.E.D.]. Deposited in PMC for immediate release. NR 55 TC 13 Z9 13 U1 2 U2 6 PU COMPANY OF BIOLOGISTS LTD PI CAMBRIDGE PA BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND SN 0950-1991 EI 1477-9129 J9 DEVELOPMENT JI Development PD AUG PY 2014 VL 141 IS 15 BP 3003 EP 3012 DI 10.1242/dev.105924 PG 10 WC Developmental Biology SC Developmental Biology GA AO4JW UT WOS:000341304600011 PM 25053433 ER PT J AU Aver'yanov, AA Lapikova, VP Pasechnik, TD Abramova, OS Gaivoronskaya, LM Kuznetsv, VV Baker, CJ AF Aver'yanov, Andrey A. Lapikova, Vera P. Pasechnik, Tatiana D. Abramova, Olga S. Gaivoronskaya, Ludmilla M. Kuznetsv, Vladimir V. Baker, C. Jacyn TI Pre-illumination of rice blast conidia induces tolerance to subsequent oxidative stress SO FUNGAL BIOLOGY LA English DT Article DE Antioxidant capacity; Fungitoxicity; Light; Oxidative stress; Rice blast spores ID PLANT DEFENSE; EXTRACELLULAR CATALASE; FUNCTIONAL-ANALYSIS; PYRICULARIA-ORYZAE; HYDROGEN-PEROXIDE; BOTRYTIS-CINEREA; ACTIVATED OXYGEN; FUNGAL MELANINS; LIGHT; DISEASE AB Many environmental factors, alone or combined, affect organisms by changing a pro-/antioxidant balance. Here we tested rice blast fungus (Magnaporthe oryzae) for possible cross-adaptations caused by relatively intense light and protecting from artificially formed reactive oxygen species (ROS) and ROS-dependent fungitoxic response of the host plant. Spore germination was found to be suppressed under 4-h and, to larger extent, 5-h illumination. The effect was diminished by antioxidants and, therefore, suggests involvement of ROS. One-hour of light did not affect spore germination, but stimulated their chemically assayed superoxide production. The illuminated spores were more tolerant (than non-illuminated ones) to artificially generated H2O2, O-2(-), or (OH)-O-center dot or to toxic diffusate of rice leaf. They also caused more severe disease symptoms if applied to leaves of the susceptible rice cultivar at low concentration. Spore diffusates decomposed hydrogen peroxide. They detoxified exogenous I:1202 and superoxide radical as well as leaf diffusates. Spore illumination increased some of these protective effects. It is suggested that short-term light led to mild oxidative stress,. which induced spore antioxidant capacity, enhancing spore tolerance to subsequent stronger oxidative stress and its aggressiveness in planta. Such tolerance depends partly on the antidotal action of spore extracellular compounds, which may also be light-stimulated. Therefore, a certain ROS-related environmental factor may adapt a fungus to other factors and so modulate its pathogenic properties. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All right reserved. C1 [Aver'yanov, Andrey A.; Lapikova, Vera P.; Pasechnik, Tatiana D.] Res Inst Phytopathol, B Vyazemy 143050, Moscow Region, Russia. [Abramova, Olga S.; Gaivoronskaya, Ludmilla M.; Kuznetsv, Vladimir V.] Russian Peoples Friendship Univ, Moscow 117813, Russia. [Baker, C. Jacyn] ARS, USDA, Beltsville, MD 20705 USA. RP Aver'yanov, AA (reprint author), Res Inst Phytopathol, B Vyazemy 143050, Moscow Region, Russia. EM andrey.a.averyanov@yandex.ru FU Agricultural Research Service of the US Department of Agriculture [2682p, 4071p] FX The work was supported by the grants ##2682p and 4071p of the Agricultural Research Service of the US Department of Agriculture mediated by the International Science and Technology Center. We thank Barbara P. Merke for her kind help in the manuscript preparation. NR 41 TC 0 Z9 0 U1 1 U2 7 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1878-6146 EI 1878-6162 J9 FUNGAL BIOL-UK JI Fungal Biol. PD AUG PY 2014 VL 118 IS 8 BP 743 EP 753 DI 10.1016/j.funbio.2014.06.003 PG 11 WC Mycology SC Mycology GA AO4ZF UT WOS:000341349200009 PM 25110136 ER PT J AU Sakhanokho, HF Islam-Faridi, MN AF Sakhanokho, Hamidou F. Islam-Faridi, M. Nurul TI Spontaneous Autotetraploidy and Its Impact on Morphological Traits and Pollen Viability in Solanum aethiopicum SO HORTSCIENCE LA English DT Article DE polyploidy; scarlet eggplant; somaclonal variation ID SOMACLONAL VARIATION; IN-VITRO; CYTOGENETIC CHARACTERIZATION; SUSPENSION-CULTURE; SHOOT REGENERATION; PLANT MORPHOLOGY; SOMATIC HYBRIDS; BACTERIAL WILT; MELONGENA L; RESISTANCE AB We report for the first time the incidence of spontaneous autotetraploidy in Solanum aethiopicunt (PI 636107). Stomatal dimensions and frequency, number of chloroplasts per guard cell, flow cytometry, and chromosome counts were used to differentiate the diploid plants from tetraploids. The impact of increased ploidy on pollen viability as assessed by in vitro germination and on selected morphological traits was evaluated. In vitro pollen germination was reduced in tetraploid plants, but no significant differences were found in fruit production per plant between diploid and tetraploid plants. Compared with the diploids, the tetraploid plants were significantly shorter and had wider leaves and smaller fruits; therefore, tetraploid S. aethiopicum plants can be valuable for future breeding programs, particularly those aiming to develop shorter, more compact plants. Moreover, some S. aethiopicum selections are grown for their edible leaves, so tetraploid plants producing large leaves would be desirable. Additionally, the availability of tetraploid S. aethiopicum could remove hybridization barriers caused by ploidy differences with other tetraploid Solanum species. C1 [Sakhanokho, Hamidou F.] ARS, USDA, Thad Cochran Southern Hort Lab, Poplarville, MS 39470 USA. [Islam-Faridi, M. Nurul] Texas A&M Univ, USDA, US Forest Serv, Southern Inst Forest Genet,Forest Tree Mol Cytoge, College Stn, TX 77843 USA. RP Sakhanokho, HF (reprint author), ARS, USDA, Thad Cochran Southern Hort Lab, 810 Highway 26 West, Poplarville, MS 39470 USA. EM Sakhanokho@ars.usda.gov NR 43 TC 1 Z9 1 U1 2 U2 8 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 997 EP 1002 PG 6 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100003 ER PT J AU Inch, S Stover, E Driggers, R Lee, RF AF Inch, Sharon Stover, Ed Driggers, Randall Lee, Richard F. TI Freeze Response of Citrus and Citrus-related Genotypes in a Florida Field Planting SO HORTSCIENCE LA English DT Article DE Aurantioideae; citrus breeding; cold-sensitive; defoliation; dieback; frost damage; Rutaceae; Toddalioideae ID TRIFOLIATA L. RAF; COLD-HARDY CITRUS; PONCIRUS-TRIFOLIATA; MEDIATED TRANSFORMATION; SWEET ORANGE; US 119; TOLERANCE; GENES; IDENTIFICATION; EXPRESSION AB A test population consisting of progenies of 92 seed-source genotypes (hereafter called "parent genotypes") of Citrus and Citrus relatives in the field in east central Florida was assessed after natural freeze events in the winters of 2010 and 2011. Eight seedlings per parent genotype were planted in a randomized complete block design; however, as a result of mortality, the number of plants assessed in some genotype groups was reduced at some or all sampling dates. The citrus diseases huanglongbing and citrus canker were endemic in the planting and may have influenced tree response to cold temperatures. Unusually low temperatures (near 4 C each winter) for east central Florida were experienced during the trial period. Defoliation and dieback were significantly greater in the winter of 2011 than in the winter of 2010. The winter in 2011 was preceded by a period of extraordinarily low temperatures in mid-December with no period of cool temperatures to allow trees to acclimate. In 2010 the average defoliation was 53% +/- 28% and less than 13% of the trees exhibited any noticeable dieback, whereas in 2011, the average defoliation and dieback were 93% +/- 17% and 51% +/- 35%, respectively. Within the genus Citrus, several progenies were identified that had 16% to 24% dieback in 2011 and these were from parent genotypes C. reticulata (CRC 2590) (23%), C. sinensis (CRC 3858) (24%), Cl maxima (CRC 3945) (16%), C. hassaku (CRC 3907 and 3942) (16% and 17%), C. aurantium (CRC 628 and 2717) (18% and 7%), C. taiwanica (CRC 2588) (21%), and C. neo-aurantium (C. obovoidea + C. unshiu graft chimera) (CRC 3816) (23%). Within other genera in the Aurantiodeae, Poncirus trifoliata (CRCs 301, 3957, 3549, and 4007), Severinia buxifolia (CRC 1497), Bergera koenigii (CRC 3165), and Glycosmis pentaphylla (CRC 3285) had the least amount of dieback, all at less than 23%. The two species within the Toddalioideae subfamily of the Rutaceae (Casimiroa edulis and Zanthoxylum ailanthoides) had among the least amount of dieback (1% and 8%, respectively). When considered by groups, the Citrons and Australian natives had the greatest amount of dieback in 2011, 68% and 65%, respectively. The trifoliates (Poncirus and hybrids) had the least dieback, ranging from 4% to 40%. The information from this study may be useful in germplasm enhancement and Citrus breeding targeting greater cold tolerance. C1 [Inch, Sharon; Stover, Ed; Driggers, Randall] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. [Lee, Richard F.] ARS, USDA, Riverside, CA 92521 USA. RP Stover, E (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM Ed.Stover@ars.usda.gov NR 50 TC 1 Z9 1 U1 2 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 1010 EP 1016 PG 7 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100005 ER PT J AU Guan, WJ Zhao, X Dickson, DW Mendes, ML Thies, J AF Guan, Wenjing Zhao, Xin Dickson, Donald W. Mendes, Maria L. Thies, Judy TI Root-knot Nematode Resistance, Yield, and Fruit Quality of Specialty Melons Grafted onto Cucumis metulifer SO HORTSCIENCE LA English DT Article DE flesh firmness; Meloidogyne; organic production; root galling; soluble solids content; rootstock ID MELOIDOGYNE-INCOGNITA; HORNED CUCUMBER; METHYL-BROMIDE; FUSARIUM-WILT; ROOTSTOCK RESISTANCE; TRIPLOID WATERMELON; FLORIDA; SOIL; PENETRATION; FUMIGATION AB Interest in specialty melons (Cucumis melo) with distinctive fruit characteristics has grown in the United States in recent years. However, disease management remains a major challenge in specialty melon production. In this study, grafting experiments were conducted to determine the effectiveness of using Cucumis metulifer, a species known for its genetic resistance to root-knot nematodes (RKNs; Meloidogyne spp.), as a potential rootstock for managing RKNs in susceptible specialty melon cultivars. In the greenhouse experiment, honeydew melon 'Honey Yellow' was grafted onto C. metulifer and inoculated with M. incognita race 1. The grafted plants exhibited significantly lower gall and egg mass indices and fewer eggs compared with non- and self-grafted 'Honey Yellow'. Cucumis metulifer was further tested as a rootstock in conventional and organic field trials using honeydew melon 'Honey Yellow' and galia melon 'Arava' as scions. 'Honey Yellow' and 'Arava' grafted onto C. metulifer exhibited significantly lower galling and reduced RKN population densities in the organic field; however, total and marketable fruit yields were not significantly different from non- and self-grafted plants. Although the improvement of RKN resistance did not translate into yield enhancements, incorporating grafted specialty melons with C. metulifer rootstock into double-cropping systems with RKN-susceptible vegetables may benefit the overall crop production by reducing RKN population densities in the soil. At the conventional field site, which was not infested with RKNs, 'Honey Yellow' grafted onto C. metulifer rootstock had a significantly lower total fruit yield than non-grafted 'Honey Yellow' plants; however, fruit yields were similar for 'Arava' grafted onto C. metulifer rootstock and non-grafted 'Arava' plants. Although no significant impacts on the fruit quality attributes of 'Honey Yellow' were observed, grafting onto C. metulifer decreased the flesh firmness of 'Arava' in both field trials and resulted in a reduction in total soluble solids content under conventional production. In summary, grafting RKN-susceptible melons onto C. metulifer rootstock offers promise for growing these specialty melons; however, more studies are needed to elucidate the scion rootstock interaction effect on fruit yield and quality. C1 [Guan, Wenjing; Zhao, Xin] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Dickson, Donald W.; Mendes, Maria L.] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Thies, Judy] ARS, USDA, US Vegetable Lab, Charleston, SC 29414 USA. RP Zhao, X (reprint author), Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. EM zxin@ufl.edu FU Southern Region SARE (Sustainable Agriculture Research and Education) Research and Education Grant [LS10-233] FX This research was funded by a Southern Region SARE (Sustainable Agriculture Research and Education) Research and Education Grant (LS10-233). NR 46 TC 4 Z9 4 U1 6 U2 27 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 1046 EP 1051 PG 6 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100011 ER PT J AU Finn, CE Strik, BC Yorgey, BM Mackey, TA Moore, PP Dossett, M Kempler, C Martin, RR Jamieson, AR Galletta, GJ AF Finn, Chad E. Strik, Bernadine C. Yorgey, Brian M. Mackey, Theodore A. Moore, Patrick P. Dossett, Michael Kempler, Chaim Martin, Robert R. Jamieson, Andrew R. Galletta, Gene J. TI 'Sweet Sunrise' Strawberry SO HORTSCIENCE LA English DT Editorial Material DE early maturity; Fragaria xananassa; fresh market; June-bearing; processing; short-day C1 [Finn, Chad E.; Mackey, Theodore A.; Martin, Robert R.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yorgey, Brian M.] Oregon State Univ, Dept Food Sci & Technol, Corvallis, OR 97331 USA. [Moore, Patrick P.] Washington State Univ, Dept Hort, Puyallup, WA 98371 USA. [Dossett, Michael; Kempler, Chaim] Agr & Agri Food Canada, Pacific Agri Food Res Ctr, Agassiz, BC V0M 1A0, Canada. [Jamieson, Andrew R.] Agr & Agri Food Canada, Atlantic Food & Hort Res Ctr, Kentville, NS B4N 1J5, Canada. [Galletta, Gene J.] ARS, USDA, Fruit Lab, Beltsville, MD 20705 USA. RP Finn, CE (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM Chad.Finn@ars.usda.gov NR 11 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 1088 EP 1092 PG 5 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100021 ER PT J AU Chaparro, JX Conner, PJ Beckman, TG AF Chaparro, Jose X. Conner, Patrick J. Beckman, Thomas G. TI 'GulfAtlas' Peach SO HORTSCIENCE LA English DT Editorial Material DE breeding; non-melting; Prunus persica C1 [Chaparro, Jose X.] Univ Florida, Dept Hort Sci, Gainesville, FL 32611 USA. [Conner, Patrick J.] Univ Georgia, Tifton, GA 31793 USA. [Beckman, Thomas G.] ARS, USDA, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. RP Beckman, TG (reprint author), ARS, USDA, SE Fruit & Tree Nut Res Lab, 21 Dunbar Rd, Byron, GA 31008 USA. EM Tom.Beckman@ars.usda.gov NR 11 TC 0 Z9 0 U1 0 U2 2 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 1093 EP 1094 PG 2 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100022 ER PT J AU Meerow, AW AF Meerow, Alan W. TI The Florida Series of Hybrid Amaryllis: Five New Hippeastrum Cultivars SO HORTSCIENCE LA English DT Editorial Material DE floriculture; flower bulb; geophyte; herbaceous perennial; Hippeastrum; hybrids; ornamentals ID COLOR C1 ARS, USDA, SHRS, Miami, FL 33158 USA. RP Meerow, AW (reprint author), ARS, USDA, SHRS, 13601 Old Cutler Rd, Miami, FL 33158 USA. EM alan.meerow@ars.usda.gov NR 15 TC 0 Z9 0 U1 1 U2 7 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 1102 EP 1107 PG 6 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100025 ER PT J AU Finn, CE Strik, BC Yorgey, BM Peterson, ME Lee, J Martin, RR Hall, HK AF Finn, Chad E. Strik, Bernadine C. Yorgey, Brian M. Peterson, Mary E. Lee, Jungmin Martin, Robert R. Hall, Harvey K. TI 'Columbia Star' Thornless Trailing Blackberry SO HORTSCIENCE LA English DT Editorial Material ID CULTIVARS; MARION C1 [Finn, Chad E.; Peterson, Mary E.; Martin, Robert R.] ARS, USDA, Hort Crops Res Unit, Corvallis, OR 97330 USA. [Strik, Bernadine C.] Oregon State Univ, Dept Hort, Corvallis, OR 97331 USA. [Yorgey, Brian M.] Oregon State Univ, Dept Food Sci, Corvallis, OR 97331 USA. [Lee, Jungmin] ARS, USDA, Hort Crops Res Unit Worksite, Parma, ID 83660 USA. [Hall, Harvey K.] New Zealand Ltd HortRes, Hort & Food Res Inst, Motueka 71989, New Zealand. RP Finn, CE (reprint author), ARS, USDA, Hort Crops Res Unit, 3420 NW Orchard Ave, Corvallis, OR 97330 USA. EM Chad.Finn@ARS.USDA.GOV RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 NR 12 TC 2 Z9 2 U1 0 U2 1 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD AUG PY 2014 VL 49 IS 8 BP 1108 EP 1112 PG 5 WC Horticulture SC Agriculture GA AO5TB UT WOS:000341407100026 ER PT J AU Niedz, RP Hyndman, SE Evens, TJ Weathersbee, AA AF Niedz, Randall P. Hyndman, Scott E. Evens, Terrence J. Weathersbee, Albert A., III TI Mineral nutrition and in vitro growth of Gerbera hybrida (Asteraceae) SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Article DE Nitrogen; Metals; Minor nutrients; Nitrate; Ammonium; Potassium; Ion-specific; Experimental design; Response surface methodology ID SHOOT REGENERATION; CULTURE-MEDIUM; TISSUE-GROWTH; JAMESONII; PLANT; EXPLANT AB The effects of mineral nutrient were examined on in vitro growth of Gerbera hybrida (G. jamesonii x G. viridifolia), specifically Gerbera hybrida cv. Pasadena. Four types of experiments were conducted to quantify the effects of mineral nutrients on four in vitro growth responses (quality, shoot number, leaf number, and shoot height) of gerbera and included groups of mineral nutrients (macros/mesos, micros, and Fe), individual salts (CuSO4 center dot 5H(2)O, MnSO4 center dot 4H(2)O, ZnSO4 center dot 7H(2)O, and Fe/EDTA), and the specific ions NO3 (-), NH4 (+), and K+. Experiments included mixture-amount designs that are essential for separating the effects of proportion and concentration. Highly significant effects were observed in all experiments, but the mineral nutrients with the largest effects varied among the four growth responses. For example, leaf number was strongly affected by the macronutrient group in one experiment and by NH4 (+) and K+, which were in the macronutrient group, in the NO3 (-)/NH4 (+)/K+ ion-specific experiment, whereas quality was strongly affected by the micronutrients ZnSO4 and Fe/EDTA. Because mineral nutrient effects varied significantly with the response measured, defining an appropriate formulation requires a clear definition of "optimal" growth. C1 [Niedz, Randall P.; Hyndman, Scott E.; Evens, Terrence J.; Weathersbee, Albert A., III] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. RP Niedz, RP (reprint author), ARS, USDA, US Hort Res Lab, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM randall.niedz@ars.usda.gov NR 28 TC 5 Z9 5 U1 5 U2 23 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2014 VL 50 IS 4 BP 458 EP 470 DI 10.1007/s11627-014-9620-6 PG 13 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA AO6AR UT WOS:000341430300009 ER PT J AU Biswas, B McKinney, M Beckman, T Johnson, S AF Biswas, B. McKinney, M. Beckman, T. Johnson, S. TI Developing Peach Orchard and Continuing In Vitro Studies for PTSL Evaluation SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Meeting Abstract CT World Forum on Biology / Joint Meeting of the Society-for-In-Vitro-Biology/Society-for-Cryobiology CY MAY 31-JUN 04, 2014 CL Savannah, GA SP Soc In Vitro Biol, Soc Cryobiol C1 [Biswas, B.; McKinney, M.] Ft Valley State Univ, Coll Agr, Ft Valley, GA 31030 USA. [Johnson, S.] Ft Valley State Univ, MS Biotechnol Program, Ft Valley, GA 31030 USA. [Beckman, T.] ARS, USDA, Byron, GA USA. EM biswasb@fvsu.edu NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2014 VL 50 IS 4 MA P-3002 BP 506 EP 507 PG 2 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA AO6AR UT WOS:000341430300018 ER PT J AU Joshee, N Parajuli, P Rimando, A AF Joshee, N. Parajuli, P. Rimando, A. TI Skullcaps: Lesser Known Medicinal Genus from Lamiaceae. SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Meeting Abstract CT World Forum on Biology / Joint Meeting of the Society-for-In-Vitro-Biology/Society-for-Cryobiology CY MAY 31-JUN 04, 2014 CL Savannah, GA SP Soc In Vitro Biol, Soc Cryobiol C1 [Joshee, N.] Ft Valley State Univ, Ft Valley, GA 31030 USA. [Parajuli, P.] Wayne State Univ, Detroit, MI USA. [Rimando, A.] ARS, USDA, Nat Prod Utilizat Res Unit, University, MS USA. EM Josheen@fvsu.edu NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2014 VL 50 IS 4 MA P-3007 BP 508 EP 508 PG 1 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA AO6AR UT WOS:000341430300023 ER PT J AU Thomson, AJ Shao, M AF Thomson, A. J. Shao, M. TI Precise Excision of Plastid DNA by the Small Serine Recombinase ParA SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Meeting Abstract CT World Forum on Biology / Joint Meeting of the Society-for-In-Vitro-Biology/Society-for-Cryobiology CY MAY 31-JUN 04, 2014 CL Savannah, GA SP Soc In Vitro Biol, Soc Cryobiol C1 [Thomson, A. J.] ARS, USDA, Albany, CA 94710 USA. [Shao, M.] Univ Calif Davis, Albany, CA USA. EM James.Thomson@ARS.USDA.GOV NR 0 TC 0 Z9 0 U1 1 U2 1 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2014 VL 50 IS 4 MA P-3020 BP 512 EP 513 PG 2 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA AO6AR UT WOS:000341430300036 ER PT J AU Jenderek, M Tanner, J Postman, J Ambruzs, B Hummer, K AF Jenderek, M. Tanner, J. Postman, J. Ambruzs, B. Hummer, K. TI Seasonal Variation in Viability of Cryopreserved Vaccinium Dormant Buds SO IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT LA English DT Meeting Abstract CT World Forum on Biology / Joint Meeting of the Society-for-In-Vitro-Biology/Society-for-Cryobiology CY MAY 31-JUN 04, 2014 CL Savannah, GA SP Soc In Vitro Biol, Soc Cryobiol C1 [Jenderek, M.; Tanner, J.] USDA ARS, NCGRP, Ft Collins, CO 80521 USA. [Postman, J.; Hummer, K.] USDA ARS, NCGR, Corvallis, OR 97333 USA. EM maria.jenderek@ars.usda.gov NR 0 TC 0 Z9 0 U1 0 U2 0 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1054-5476 EI 1475-2689 J9 IN VITRO CELL DEV-PL JI In Vitro Cell. Dev. Biol.-Plant PD AUG PY 2014 VL 50 IS 4 MA P-3024 BP 514 EP 514 PG 1 WC Plant Sciences; Cell Biology; Developmental Biology SC Plant Sciences; Cell Biology; Developmental Biology GA AO6AR UT WOS:000341430300041 ER PT J AU Leiva-Valenzuela, GA Lu, RF Aguiler, JM AF Leiva-Valenzuela, Gabriel A. Lu, Renfu Miguel Aguiler, Jose TI Assessment of internal quality of blueberries using hyperspectral transmittance and reflectance images with whole spectra or selected wavelengths SO INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES LA English DT Article DE Hyperspectral imaging; Wavelength selection; Blueberry; Firmness; Soluble solids content; Reflectance; Transmittance; Interval partial least squares ID SOLUBLE SOLIDS CONTENT; APPLE FRUIT FIRMNESS; FT-NIR SPECTROSCOPY; NONDESTRUCTIVE MEASUREMENT; SCATTERING IMAGES; PREDICTION; CLASSIFICATION; INFESTATION; REGRESSION; SEPARATION AB Hyperspectral imaging has been used in previous studies for assessing firmness and soluble solids content of fresh fruit. To assess the applicability of this technique for automatic sorting and grading of blueberries, we investigated different sensing modes (i.e., reflectance and transmittance), evaluated the effect of fruit orientation on fruit quality prediction, and developed robust prediction models with fewer wavelengths. In this study, a hyperspectral imaging system was used to acquire reflectance and transmittance images from 420 blueberries in three fruit orientations (i.e., stem end, calyx end and equator) for the spectral region of 400-1000 nm. Mean spectra were extracted from the hyperspectral images of each blueberry. Calibration models for soluble solids content (SSC) and firmness index (FI) were developed using partial least squares regression for the reflectance and transmittance spectra as well as their combination. Further, interval partial least squares (iPLS) regression with 10 different intervals of nine wavelengths was used to reduce the spectral dimensionality. Overall, reflectance gave better results (the best correlation for prediction (R-p) of 0.90 for SSC and 0.78 for El) than transmittance (R-p of 0.76 for SSC and 0.64 for FI). For reflectance, SSC and FI predictions for the stem end orientation were better than for the other two orientations, while fruit orientation had little or insignificant effect on transmittance predictions. Combination of reflectance and transmittance spectra did not yield improved prediction results for both SSC and H. On average, the prediction errors for iPLS increased by only 5%, compared to PLS for the whole spectra. The research demonstrated that it is feasible to use hyperspectral imaging technique for prediction of internal quality of blueberries with a few selected wavelengths with results similar to that with whole spectral information. Industrial relevance: Because of the distance traveled from the South to the North hemisphere, it is especially important to perform internal and external quality determination for individual fresh blueberries to ensure their quality upon arrival at the destination. Soluble solids content and firmness are important fruit quality parameters. Hyperspectral imaging has emerged as a new technique for quality and safety inspection of food and agricultural products and could be useful for blueberry quality assessment. However, there are several limitations to be afforded before: technique implementation velocity since this method uses multiple images from contiguous wavelengths (increasing computational costs), fruit light interaction, and fruit orientation effect between others. Specifically, the submitted manuscript presents results in order to demonstrate the hyperspectral imaging technique feasibility with a few selected wavelengths to achieve acceptable results for the prediction of internal quality of blueberries, thus, this would make it possible to implement the technique in the near future for online commercial sorting and grading of blueberries. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Leiva-Valenzuela, Gabriel A.; Miguel Aguiler, Jose] Pontificia Univ Catolica Chile, Dept Chem & Bioproc Engn, Santiago, Chile. [Leiva-Valenzuela, Gabriel A.; Lu, Renfu] Michigan State Univ, USDA ARS, E Lansing, MI 48824 USA. RP Leiva-Valenzuela, GA (reprint author), Pontificia Univ Catolica Chile, Dept Chem & Bioproc Engn, Ave Vicuna Mackenna,4680 Macul, Santiago, Chile. EM gmleiva@uc.cl FU National Commission for Science and Technology (CONICYT) of Chile FX Mr. Gabriel A. Leiva-Valenzuela thanks the National Commission for Science and Technology (CONICYT) of Chile for providing a fellowship, which allowed him to carry out the research in the USDA/ARS postharvest engineering laboratory at Michigan State University in the USA. The authors also acknowledge the helpful experimental aid from Ms. Miranda Sperry and Ms. Stacey Stark, graduate students in Biosystems Engineering at Michigan State University. NR 57 TC 19 Z9 21 U1 5 U2 46 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1466-8564 EI 1878-5522 J9 INNOV FOOD SCI EMERG JI Innov. Food Sci. Emerg. Technol. PD AUG PY 2014 VL 24 SI SI BP 2 EP 13 DI 10.1016/j.ifset.2014.02.006 PG 12 WC Food Science & Technology SC Food Science & Technology GA AO7TE UT WOS:000341554700002 ER PT J AU Wang, HF Blumberg, JB Chen, CYO Choi, SW Corcoran, MP Harris, SS Jacques, PF Kristo, AS Lai, CQ Lamon-Fava, S Matthan, NR McKay, DL Meydani, M Parnell, LD Prokopy, MP Scott, TM Lichtenstein, AH AF Wang, Huifen Blumberg, Jeffrey B. Chen, C. -Y. Oliver Choi, Sang-Woon Corcoran, Michael P. Harris, Susan S. Jacques, Paul F. Kristo, Aleksandra S. Lai, Chao-Qiang Lamon-Fava, Stefania Matthan, Nirupa R. McKay, Diane L. Meydani, Mohsen Parnell, Laurence D. Prokopy, Max P. Scott, Tammy M. Lichtenstein, Alice H. TI Dietary modulators of statin efficacy in cardiovascular disease and cognition SO MOLECULAR ASPECTS OF MEDICINE LA English DT Review DE Statin; Diet; Nutrient; Interaction; Cardiovascular disease; Cognition ID CORONARY-HEART-DISEASE; HMG-COA-REDUCTASE; DENSITY-LIPOPROTEIN CHOLESTEROL; POLYUNSATURATED FATTY-ACIDS; RANDOMIZED CONTROLLED-TRIAL; C-REACTIVE PROTEIN; ANION-TRANSPORTING POLYPEPTIDE; GENOME-WIDE ASSOCIATION; VITAMIN-D DEFICIENCY; EXTENDED-RELEASE NIACIN AB Cardiovascular disease remains the leading cause of morbidity and mortality in the United States and other developed countries, and is fast growing in developing countries, particularly as life expectancy in all parts of the world increases. Current recommendations for the prevention of cardiovascular disease issued jointly from the American Academy of Cardiology and American Heart Association emphasize that lifestyle modification should be incorporated into any treatment plan, including those on statin drugs. However, there is a dearth of data on the interaction between diet and statins with respect to additive, complementary or antagonistic effects. This review collates the available data on the interaction of statins and dietary patterns, cognition, genetics and individual nutrients, including vitamin D. niacin, omega-3 fatty acids, fiber, phytochemicals (polyphenols and stanols) and alcohol. Of note, although the available data is summarized, the scope is limited, conflicting and disparate. In some cases it is likely there is unrecognized synergism. Virtually no data are available describing the interactions of statins with dietary components or dietary pattern in subgroups of the population, particularly those who may benefit most were positive effects identified. Hence, it is virtually impossible to draw any firm conclusions at this time. Nevertheless, this area is important because were the effects of statins and diet additive or synergistic harnessing the effect could potentially lead to the use of a lower intensity statin or dose. (C) 2014 Published by Elsevier Ltd. C1 [Wang, Huifen] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Choi, SW (reprint author), CHA Univ, Sch Med, CHAUM Life Ctr, 442 Dosan Daero, Seoul 135948, South Korea. EM sang.choi@cha.ac.kr FU Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University cluster pilot funds; USDA [58-1950-0-014] FX This work was supported by Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University cluster pilot funds and USDA Agreement No. 58-1950-0-014. NR 408 TC 2 Z9 2 U1 4 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0098-2997 EI 1872-9452 J9 MOL ASPECTS MED JI Mol. Asp. Med. PD AUG PY 2014 VL 38 SI SI BP 1 EP 53 DI 10.1016/j.mam.2014.04.001 PG 53 WC Biochemistry & Molecular Biology; Medicine, Research & Experimental SC Biochemistry & Molecular Biology; Research & Experimental Medicine GA AO7NA UT WOS:000341538700001 PM 24813475 ER PT J AU Kyrpides, NC Hugenholtz, P Eisen, JA Woyke, T Goker, M Parker, CT Amann, R Beck, BJ Chain, PSG Chun, J Colwell, RR Danchin, A Dawyndt, P Dedeurwaerdere, T DeLong, EF Detter, JC De Vos, P Donohue, TJ Dong, XZ Ehrlich, DS Fraser, C Gibbs, R Gilbert, J Gilna, P Glockner, FO Jansson, JK Keasling, JD Knight, R Labeda, D Lapidus, A Lee, JS Li, WJ Ma, JC Markowitz, V Moore, ERB Morrison, M Meyer, F Nelson, KE Ohkuma, M Ouzounis, CA Pace, N Parkhill, J Qin, N Rossello-Mora, R Sikorski, J Smith, D Sogin, M Stevens, R Stingl, U Suzuki, K Taylor, D Tiedje, JM Tindall, B Wagner, M Weinstock, G Weissenbach, J White, O Wang, J Zhang, LX Zhou, YG Field, D Whitman, WB Garrity, GM Klenk, HP AF Kyrpides, Nikos C. Hugenholtz, Philip Eisen, Jonathan A. Woyke, Tanja Goeker, Markus Parker, Charles T. Amann, Rudolf Beck, Brian J. Chain, Patrick S. G. Chun, Jongsik Colwell, Rita R. Danchin, Antoine Dawyndt, Peter Dedeurwaerdere, Tom DeLong, Edward F. Detter, John C. De Vos, Paul Donohue, Timothy J. Dong, Xiu-Zhu Ehrlich, Dusko S. Fraser, Claire Gibbs, Richard Gilbert, Jack Gilna, Paul Glockner, Frank Oliver Jansson, Janet K. Keasling, Jay D. Knight, Rob Labeda, David Lapidus, Alla Lee, Jung-Sook Li, Wen-Jun Ma, Juncai Markowitz, Victor Moore, Edward R. B. Morrison, Mark Meyer, Folker Nelson, Karen E. Ohkuma, Moriya Ouzounis, Christos A. Pace, Norman Parkhill, Julian Qin, Nan Rossello-Mora, Ramon Sikorski, Johannes Smith, David Sogin, Mitch Stevens, Rick Stingl, Uli Suzuki, Ken-ichiro Taylor, Dorothea Tiedje, Jim M. Tindall, Brian Wagner, Michael Weinstock, George Weissenbach, Jean White, Owen Wang, Jun Zhang, Lixin Zhou, Yu-Guang Field, Dawn Whitman, William B. Garrity, George M. Klenk, Hans-Peter TI Genomic Encyclopedia of Bacteria and Archaea: Sequencing a Myriad of Type Strains SO PLOS BIOLOGY LA English DT Editorial Material ID MICROBIAL DIVERSITY; MINIMUM INFORMATION; PHYLOGENY; STANDARDS; PROJECT; TREE; MICROORGANISMS; NOMENCLATURE; PUBLICATION; PROKARYOTES AB Microbes hold the key to life. They hold the secrets to our past (as the descendants of the earliest forms of life) and the prospects for our future (as we mine their genes for solutions to some of the planet's most pressing problems, from global warming to antibiotic resistance). However, the piecemeal approach that has defined efforts to study microbial genetic diversity for over 20 years and in over 30,000 genome projects risks squandering that promise. These efforts have covered less than 20% of the diversity of the cultured archaeal and bacterial species, which represent just 15% of the overall known prokaryotic diversity. Here we call for the funding of a systematic effort to produce a comprehensive genomic catalog of all cultured Bacteria and Archaea by sequencing, where available, the type strain of each species with a validly published name (currently, 11,000). This effort will provide an unprecedented level of coverage of our planet's genetic diversity, allow for the large-scale discovery of novel genes and functions, and lead to an improved understanding of microbial evolution and function in the environment. C1 [Kyrpides, Nikos C.; Woyke, Tanja; Markowitz, Victor] DOE Joint Genome Inst, Walnut Creek, CA 94598 USA. [Kyrpides, Nikos C.] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah, Saudi Arabia. [Hugenholtz, Philip] Univ Queensland, Sch Chem & Mol Biosci, Australian Ctr Ecogenom Res, Brisbane, Qld, Australia. [Eisen, Jonathan A.] Univ Calif Davis, Davis, CA 95616 USA. [Goeker, Markus; Sikorski, Johannes; Tindall, Brian; Klenk, Hans-Peter] DSMZ German Collect Microorganisms & Cell Culture, Braunschweig, Germany. [Parker, Charles T.; Taylor, Dorothea; Garrity, George M.] NamesforLife LLC, E Lansing, MI USA. [Amann, Rudolf; Glockner, Frank Oliver] Max Planck Inst Marine Microbiol, Bremen, Germany. [Beck, Brian J.] Amer Type Culture Collect ATCC, Manassas, VA USA. [Chain, Patrick S. G.; Detter, John C.] Los Alamos Natl Lab, Biosci Div, Los Alamos, NM USA. [Chun, Jongsik] Seoul Natl Univ, Sch Biol Sci, Seoul, South Korea. [Chun, Jongsik] Seoul Natl Univ, Chunlab Inc, Seoul, South Korea. [Colwell, Rita R.] Univ Maryland, College Pk, MD 20742 USA. [Colwell, Rita R.] Johns Hopkins Univ, Johns Hopkins Bloomberg Sch Publ Hlth, Baltimore, MD USA. [Danchin, Antoine] Genopole, AMAbiot SAS, Evry, France. [Dawyndt, Peter; De Vos, Paul] Univ Ghent, Dept Appl Math & Comp Sci, B-9000 Ghent, Belgium. [Dedeurwaerdere, Tom] Catholic Univ Louvain, Ctr Philosophy Law, Louvain La Neuve, Belgium. [DeLong, Edward F.] MIT, Dept Civil & Environm Engn, Cambridge, MA 02139 USA. [DeLong, Edward F.] MIT, Dept Biol Engn, Cambridge, MA 02139 USA. [De Vos, Paul] Univ Ghent, BCCM LMG Bacteria Collect, Microbiol Lab, B-9000 Ghent, Belgium. [Donohue, Timothy J.] Univ Wisconsin, Great Lakes Bioenergy Res Ctr, Madison, WI USA. [Dong, Xiu-Zhu; Zhang, Lixin] Chinese Acad Sci, Bioresource Ctr BRC Inst Microbiol, Beijing 100864, Peoples R China. [Ehrlich, Dusko S.] Inst Natl Rech Agronom, Jouy En Josas, France. [Fraser, Claire; White, Owen] Univ Maryland, Sch Med, Inst Genome Sci, Baltimore, MD 21201 USA. [Gibbs, Richard] Baylor Coll Med, Human Genome Sequencing Ctr, Houston, TX 77030 USA. [Gilbert, Jack] Argonne Natl Lab, Inst Genom & Syst Biol, Argonne, IL 60439 USA. [Gilna, Paul] Oak Ridge Natl Lab, BESC, Knoxville, TN USA. [Glockner, Frank Oliver] Jacobs Univ Bremen gGmbH, Bremen, Germany. [Jansson, Janet K.; Keasling, Jay D.; Markowitz, Victor] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Berkeley, CA 94720 USA. [Keasling, Jay D.] JBEI, Berkeley, CA USA. [Knight, Rob] Univ Colorado, Howard Hughes Med Inst, Boulder, CO 80309 USA. [Knight, Rob] Univ Colorado, Dept Chem & Biochem, Boulder, CO 80309 USA. [Labeda, David] ARS, USDA, Natl Ctr Agr Utilizat Res, Peoria, IL USA. [Lapidus, Alla] St Petersburg State Univ, Theodosius Dobzhansky Ctr Genome Bioinformat, St Petersburg 199034, Russia. [Lapidus, Alla] St Petersburg Acad Univ, Algorithm Biol Lab, St Petersburg, Russia. [Lee, Jung-Sook] Korea Res Inst Biosci & Biotechnol KRIBB, Korean Collect Type Cultures KCTC, Taejon, South Korea. [Li, Wen-Jun] Minist Educ, Key Lab Microbial Resources, Kunming, Peoples R China. [Ma, Juncai; Zhou, Yu-Guang] Chinese Acad Sci, China Gen Microbiol Culture Collect Ctr CGMCC, Inst Microbiol, Beijing, Peoples R China. [Moore, Edward R. B.] Univ Gothenburg, Sahlgrenska Acad, CCUG Culture Collect Univ Gothenburg, Gothenburg, Sweden. [Morrison, Mark] Univ Queensland, Diamantina Inst, St Lucia, Qld, Australia. [Meyer, Folker; Stevens, Rick] Argonne Natl Lab, Math & Comp Sci Div, Argonne, IL 60439 USA. [Nelson, Karen E.] J Craig Venter Inst, Rockville, MD USA. [Ohkuma, Moriya] Japan Collect Microorganisms, Riken Bioresource Ctr, Wako, Saitama, Japan. [Ouzounis, Christos A.] Ctr Res & Technol, Chem Proc & Energy Resources Inst, Thessaloniki, Greece. [Ouzounis, Christos A.] Univ Toronto, Donnelly Ctr Cellular & Biomol Res, Toronto, ON, Canada. [Pace, Norman] Univ Colorado, Dept Mol Cellular & Dev Biol, Boulder, CO 80309 USA. [Parkhill, Julian] Wellcome Trust Sanger Inst, Cambridge, England. [Qin, Nan; Wang, Jun] Zhejiang Univ, Coll Med, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis, Hangzhou 310003, Zhejiang, Peoples R China. [Rossello-Mora, Ramon] CSIC UIB, Inst Mediterrani Estudis Avancats IMEDEA, Illes Balears, Spain. [Smith, David] CABI, Egham, Surrey, England. [Sogin, Mitch] MBL, Josephine Bay Paul Ctr Comparat Evolut & Mol Biol, Woods Hole, MA USA. [Stingl, Uli] KAUST, Red Sea Res Ctr, Thuwal, Saudi Arabia. [Suzuki, Ken-ichiro] NBRC, Kisarazu, Chiba, Japan. [Tiedje, Jim M.; Garrity, George M.] Michigan State Univ, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA. [Wagner, Michael] Univ Vienna, Dept Microbial Ecol, Vienna, Austria. [Weinstock, George] Jackson Lab Genom Med, Farmington, CT USA. [Weissenbach, Jean] CEA, Evry, France. [Wang, Jun] Univ Copenhagen, Dept Biol, Copenhagen, Denmark. [Zhang, Lixin] Chinese Acad Sci, Chinese Acad Sci Key Lab Pathogen Microbiol & Imm, Inst Microbiol, Beijing, Peoples R China. [Field, Dawn] UK Nat Environm Res Council NERC, Environm Bioinformat Ctr, Oxford, England. [Whitman, William B.] Univ Georgia, Dept Microbiol, Athens, GA 30602 USA. RP Kyrpides, NC (reprint author), DOE Joint Genome Inst, Walnut Creek, CA 94598 USA. EM nckyrpides@lbl.gov; Hans-Peter.Klenk@dsmz.de RI Kyrpides, Nikos/A-6305-2014; Fac Sci, KAU, Biol Sci Dept/L-4228-2013; Faculty of, Sciences, KAU/E-7305-2017; Garrity, George/F-7551-2013; Keasling, Jay/J-9162-2012; Ohkuma, Moriya/A-8100-2011; Wagner, Michael/A-7801-2011; Ouzounis, Christos/G-2302-2010; Gilna, Paul/I-3608-2016; Knight, Rob/D-1299-2010; Rossello-Mora, Ramon/L-4650-2014; Amann, Rudolf/C-6534-2014; De Vos, Paul/J-5392-2013; Hugenholtz, Philip/G-9608-2011; Parkhill, Julian/G-4703-2011 OI Danchin, Antoine/0000-0002-6350-5001; Kyrpides, Nikos/0000-0002-6131-0462; Garrity, George/0000-0002-4465-7034; Colwell, Rita R./0000-0001-5432-1502; Fraser, Claire/0000-0003-1462-2428; Stingl, Uli/0000-0002-0684-2597; Eisen, Jonathan A./0000-0002-0159-2197; Chain, Patrick/0000-0003-3949-3634; Dawyndt, Peter/0000-0002-1623-9070; Keasling, Jay/0000-0003-4170-6088; Wagner, Michael/0000-0002-9778-7684; Gilna, Paul/0000-0002-6542-0191; Rossello-Mora, Ramon/0000-0001-8253-3107; Amann, Rudolf/0000-0002-0846-7372; Parkhill, Julian/0000-0002-7069-5958 NR 44 TC 56 Z9 57 U1 5 U2 68 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1545-7885 J9 PLOS BIOL JI PLoS. Biol. PD AUG PY 2014 VL 12 IS 8 AR e1001920 DI 10.1371/journal.pbio.1001920 PG 7 WC Biochemistry & Molecular Biology; Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics GA AO7HI UT WOS:000341523000003 PM 25093819 ER PT J AU Wickham, J Homer, C Vogelmann, J McKerrow, A Mueller, R Herold, N Coulston, J AF Wickham, James Homer, Collin Vogelmann, James McKerrow, Alexa Mueller, Rick Herold, Nate Coulston, John TI The Multi-Resolution Land Characteristics (MRLC) Consortium-20 Years of Development and Integration of USA National Land Cover Data SO REMOTE SENSING LA English DT Article DE C-CAP; change detection; Cropland Data Layer (CDL); GAP; LANDFIRE; NLCD ID CONTERMINOUS UNITED-STATES; THEMATIC ACCURACY; IMPERVIOUS SURFACE; NLCD 2001; ANCILLARY DATA; PERCENT TREE; IMAGERY; COMPLETION; EMERGENCE; CANOPY AB The Multi-Resolution Land Characteristics (MRLC) Consortium demonstrates the national benefits of USA Federal collaboration. Starting in the mid-1990s as a small group with the straightforward goal of compiling a comprehensive national Landsat dataset that could be used to meet agencies' needs, MRLC has grown into a group of 10 USA Federal Agencies that coordinate the production of five different products, including the National Land Cover Database (NLCD), the Coastal Change Analysis Program (C-CAP), the Cropland Data Layer (CDL), the Gap Analysis Program (GAP), and the Landscape Fire and Resource Management Planning Tools (LANDFIRE). As a set, the products include almost every aspect of land cover from impervious surface to detailed crop and vegetation types to fire fuel classes. Some products can be used for land cover change assessments because they cover multiple time periods. The MRLC Consortium has become a collaborative forum, where members share research, methodological approaches, and data to produce products using established protocols, and we believe it is a model for the production of integrated land cover products at national to continental scales. We provide a brief overview of each of the main products produced by MRLC and examples of how each product has been used. We follow that with a discussion of the impact of the MRLC program and a brief overview of future plans. C1 [Wickham, James] USA Environm Protect Agcy, Natl Exposure Res Lab E243 05, Res Triangle Pk, NC 27711 USA. [Homer, Collin; Vogelmann, James] USA Geol Survey, Earth Resources Observat & Sci EROS Ctr, Sioux Falls, SD 57198 USA. [McKerrow, Alexa] N Carolina State Univ, USA Geol Survey, Raleigh, NC 27695 USA. [Mueller, Rick] USA Dept Agr, Natl Agr Stat Serv, Fairfax, VA 22030 USA. [Herold, Nate] NOAA, Coastal Serv Ctr, Charleston, SC 29405 USA. [Coulston, John] USDA Forest Serv, Southern Res Stn, Knoxville, TN 37919 USA. RP Wickham, J (reprint author), USA Environm Protect Agcy, Natl Exposure Res Lab E243 05, Res Triangle Pk, NC 27711 USA. EM wickham.james@epa.gov; homer@usgs.gov; vogel@usgs.gov; alexa_mckerrow@ncsu.edu; rick_mueller@nass.usda.gov; nate.herold@noaa.gov; jcoulston@fs.fed.us OI Vogelmann, James/0000-0002-0804-5823 FU The USA Environmental Protection Agency through its Office of Research and Development FX The USA Environmental Protection Agency through its Office of Research and Development partially funded and collaborated in the research described here. It has been subjected to agency review and approved for publication. Approval does not signify that the contents reflect the views of the Agency. We thank Jeffery Eidenshink and Don Ohlen of the USGS and five anonymous journal reviewers for the helpful comments on earlier versions of the paper. NR 62 TC 4 Z9 5 U1 5 U2 21 PU MDPI AG PI BASEL PA POSTFACH, CH-4005 BASEL, SWITZERLAND SN 2072-4292 J9 REMOTE SENS-BASEL JI Remote Sens. PD AUG PY 2014 VL 6 IS 8 BP 7424 EP 7441 DI 10.3390/rs6087424 PG 18 WC Remote Sensing SC Remote Sensing GA AO7FV UT WOS:000341518700029 ER PT J AU Riggins, JJ Little, NS Eckhardt, LG AF Riggins, John J. Little, Nathan S. Eckhardt, Lori G. TI Correlation between infection by ophiostomatoid fungi and the presence of subterranean termites in loblolly pine (Pinus taeda L.) roots SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Blue-stain fungi; Leptographium; loblolly pine roots; ophiostomatoid; Pinus taeda L.; subterranean termites ID BLUE-STAIN FUNGI; RETICULITERMES-FLAVIPES; SP-NOV; DECLINE; WOOD; LEPTOGRAPHIUM; PREFERENCE; INSECTS; VERTICICLADIELLA; RHINOTERMITIDAE AB 1 Observations of subterranean termites feeding in pine sapwood containing ophiostomatoid fungi prompted the present study aiming to investigate the effect of infection by Leptographium fungi on the probability of encountering subterranean termites in loblolly pine (Pinus taeda L.) roots. 2 Root samples were collected from 2350 loblolly pine trees in Alabama, Texas, Louisiana and Mississippi using hand tools. The presence or absence of subterranean termites in the roots was recorded. Samples were plated on malt extract agar and cycloheximide-streptomycin malt agar and incubated to determine which, if any, fungal species were present. 3 The presence of Leptographium procerum, Leptographium terebrantis or both was significantly associated with an increased presence of subterranean termites in loblolly pine roots. 4 The results obtained in the present study indicate that complex ecological interactions may exist between ophiostomatoid fungi, as carried by root-feeding bark beetles, and subterranean termites. C1 [Riggins, John J.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Little, Nathan S.] USDA ARS, Southern Insect Management Unit, Stoneville, MS 38776 USA. [Eckhardt, Lori G.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. RP Riggins, JJ (reprint author), Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Box 9775, Mississippi State, MS 39762 USA. EM jriggins@entomology.msstate.edu FU USDA Forest Service Southern Research Station; Mississippi Agricultural and Forestry Experiment Station; Forest Health Cooperative at Auburn University FX This research was funded by the USDA Forest Service Southern Research Station; the Mississippi Agricultural and Forestry Experiment Station; and the Forest Health Cooperative at Auburn University. NR 46 TC 1 Z9 1 U1 5 U2 11 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD AUG PY 2014 VL 16 IS 3 BP 260 EP 264 DI 10.1111/afe.12053 PG 5 WC Entomology SC Entomology GA AN5YB UT WOS:000340667700005 ER PT J AU Siegert, NW Secord, T McCullough, DG AF Siegert, Nathan W. Secord, Theresa McCullough, Deborah G. TI Submersion as a tactic to prevent emergence of emerald ash borer Agrilus planipennis from black ash logs SO AGRICULTURAL AND FOREST ENTOMOLOGY LA English DT Article DE Basket ash; cultural keystone species; Fraxinus nigra; invasive forest insect; traditional arts ID BUPRESTIDAE; COLEOPTERA; DISPERSAL; SURVIVAL; SITES AB 1 Black ash (Fraxinus nigra Marshall) trees are an important cultural resource and provide the wood used for traditional contemporary basketry by numerous Native American and First Nation tribes in the U.S.A. and Canada. The invasion and subsequent spread of emerald ash borer Agrilus planipennis Fairmaire (Coleoptera: Buprestidae) threatens the ash resource in North America and the basketry tradition. Submersion of infested ash logs has been proposed as a potential tactic to control A. planipennis emergence, although the duration of submergence required to prevent adult beetle emergence is unknown. 2 We assessed the survivorship of A. planipennis prepupae through adult beetle emergence in green ash (F. pennsylvanica Marshall) bolts submerged at weekly intervals for up to 6 weeks and in black ash bolts submerged at 4-week intervals for up to 24 weeks. The moisture content of sapwood at depths where prepupal chambers are commonly found was quantified in the black ash bolts after 0, 12 and 24 weeks of submergence. 3 Mortality of A. planipennis from prepupa to adult was low (5.7 +/- 2.3%) for the first 6 weeks of submersion but rapidly increased to 100% in green ash and black ash bolts submerged for 8 weeks or longer. In black ash, the outer 1.3 cm of wood became highly saturated after submersion for 12 weeks (81.0 +/- 4.5%) and moisture increased only slightly after 24weeks (83.9 +/- 2.8%). 4 The results obtained in the present study indicate that A. planipennis prepupae in green ash and black ash bolts can survive complete submersion for extended periods of time (i.e. at least 6 weeks) and successfully emerge as adults afterwards. Logs submerged for at least 8 weeks, however, produced no live A. planipennis beetles, indicating that submersion represents an efficacious control option. C1 [Siegert, Nathan W.; McCullough, Deborah G.] Michigan State Univ, Dept Entomol, E Lansing, MI 48824 USA. [Secord, Theresa] Maine Indian Basketmakers Alliance, Old Town, ME 04468 USA. [McCullough, Deborah G.] Michigan State Univ, Dept Forestry, E Lansing, MI 48824 USA. RP Siegert, NW (reprint author), US Forest Serv, Northeastern Area State & Private Forestry, 271 Mast Rd, Durham, NH 03824 USA. EM nwsiegert@fs.fed.us FU USDA Forest Service, Northeastern Area State & Private Forestry, Forest Health Protection FX We thank Claire Siegert (MSU) for her assistance with changing the water in the submersion containers used for the black ash 24-week submersion study and Jacob Bournay (MSU) for assisting with the log dissection. We also thank Kelly Church (Grand Traverse Band of Ottawa and Chippewa Indians, Michigan) and Jeremy Frey (Passamaquoddy Tribe of Indian Township, Maine), who generously shared traditional ecological knowledge related to black ash and Anishinabe and Wabanaki basketry, respectively. Funding for this project was provided by USDA Forest Service, Northeastern Area State & Private Forestry, Forest Health Protection. NR 13 TC 1 Z9 1 U1 1 U2 28 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1461-9555 EI 1461-9563 J9 AGR FOREST ENTOMOL JI Agric. For. Entomol. PD AUG PY 2014 VL 16 IS 3 BP 321 EP 325 DI 10.1111/afe.12057 PG 5 WC Entomology SC Entomology GA AN5YB UT WOS:000340667700012 ER PT J AU Tammen, SA Dolnikowski, GG Ausman, LM Liu, ZH Sauer, J Friso, S Choi, SW AF Tammen, Stephanie A. Dolnikowski, Gregory G. Ausman, Lynne M. Liu, Zhenhua Sauer, Julia Friso, Simonetta Choi, Sang-Woon TI Aging and Alcohol Interact to Alter Hepatic DNA Hydroxymethylation SO ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH LA English DT Article DE Aging; Alcohol; DNA Hydroxymethylation; Liver; Mouse ID EMBRYONIC STEM-CELLS; GENOMIC DNA; EPIGENETIC REGULATION; GENE-TRANSCRIPTION; OXIDATIVE STRESS; HUMAN CANCERS; RAT COLON; 5-HYDROXYMETHYLCYTOSINE; METHYLATION; MOUSE AB Background: Aging and chronic alcohol consumption are both modifiers of DNA methylation, but it is not yet known whether chronic alcohol consumption also alters DNA hydroxymethylation, a newly discovered epigenetic mark produced by oxidation of methylcytosine. Furthermore, it has not been tested whether aging and alcohol interact to modify this epigenetic phenomenon, thereby having an independent effect on gene expression. Methods: Old (18 months) and young (4 months) male C57BL/6 mice were pair-fed either a Lieber-DeCarli liquid diet with alcohol (18% of energy) or an isocaloric Lieber-DeCarli control diet for 5 weeks. Global DNA hydroxymethylation and DNA methylation were analyzed from hepatic DNA using a new liquid chromatography-tandem mass spectrometry method. Hepatic mRNA expression of the Tet enzymes were measured via quantitative real-time polymerase chain reaction. Results: In young mice, mild chronic alcohol exposure significantly reduced global DNA hydroxymethylation compared with control mice (0.22 +/- 0.01 vs. 0.29 +/- 0.06%, p = 0.004). Alcohol did not significantly alter hydroxymethylcytosine levels in old mice. Old mice fed the control diet showed decreased global DNA hydroxymethylation compared with young mice fed the control diet (0.24 +/- 0.02 vs. 0.29 +/- 0.06%, p = 0.04). This model suggests an interaction between aging and alcohol in determining DNA hydroxymethylation (p(interaction) = 0.009). Expression of Tet2 and Tet3 was decreased in the old mice relative to the young (p < 0.005). Conclusions: The observation that alcohol alters DNA hydroxymethylation indicates a new epigenetic effect of alcohol. This is the first study demonstrating the interactive effects of chronic alcohol consumption and aging on DNA hydroxymethylation. C1 [Tammen, Stephanie A.; Dolnikowski, Gregory G.; Ausman, Lynne M.; Liu, Zhenhua; Sauer, Julia; Choi, Sang-Woon] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tammen, Stephanie A.; Dolnikowski, Gregory G.; Ausman, Lynne M.; Choi, Sang-Woon] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Liu, Zhenhua; Choi, Sang-Woon] Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Amherst, MA 01003 USA. [Friso, Simonetta] Univ Verona, Sch Med, I-37100 Verona, Italy. [Choi, Sang-Woon] CHA Univ, Sch Med, Chaum Life Ctr, Seoul 135948, South Korea. RP Choi, SW (reprint author), CHA Univ, Sch Med, Chaum Life Ctr, 442 Dosan Daero, Seoul 135948, South Korea. EM sang.choi@cha.ac.kr FU U.S. Department of Agriculture [51000-074-01S]; National Institute of Health [R21-AA016681, R01 AG025834] FX This material is based upon work supported by the U.S. Department of Agriculture, under agreement No. 51000-074-01S. Any opinions, findings, conclusion, or recommendations expressed in this publication are those of the author (s) and do not necessarily reflect the view of the U. S. Department of Agriculture. This project has been supported in part by the National Institute of Health Grants R21-AA016681 and R01 AG025834 (S-WC). NR 52 TC 4 Z9 4 U1 1 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-6008 EI 1530-0277 J9 ALCOHOL CLIN EXP RES JI Alcoholism (NY) PD AUG PY 2014 VL 38 IS 8 BP 2178 EP 2185 DI 10.1111/acer.12477 PG 8 WC Substance Abuse SC Substance Abuse GA AO2UQ UT WOS:000341181900006 PM 25070523 ER PT J AU Kallapura, G Kogut, MH Morgan, MJ Pumford, NR Bielke, LR Wolfenden, AD Faulkner, OB Latorre, JD Menconi, A Hernandez-Velasco, X Kuttappan, VA Hargis, BM Tellez, G AF Kallapura, G. Kogut, M. H. Morgan, M. J. Pumford, N. R. Bielke, L. R. Wolfenden, A. D. Faulkner, O. B. Latorre, J. D. Menconi, A. Hernandez-Velasco, X. Kuttappan, V. A. Hargis, B. M. Tellez, G. TI Fate of Salmonella Senftenberg in broiler chickens evaluated by challenge experiments SO AVIAN PATHOLOGY LA English DT Article ID HIGH-PATHOGENICITY ISLAND; SURFACE MACROPHAGES; RESPIRATORY-TRACT; HATCHING CABINETS; TYPHIMURIUM; POULTRY; ENTERITIDIS; DEPOSITION; INFECTION; SURVIVAL AB Experimental and epidemiological evidence has indicated the respiratory route to be a potential portal of entry for salmonellas in poultry. The purpose of this study was to evaluate and compare the infectivity of Salmonella enterica serovar Senftenberg following oral gavage, intratracheal or intravenous challenge in chickens. Seven-day-old chicks were challenged with either 104 or 106 colony-forming units of S. Senftenberg per chick by oral gavage, intratracheal or intravenous challenge, respectively, in two independent trials. Chickens were humanely killed 24 h post challenge and S. Senftenberg was cultured and enumerated from caecal contents, caecal tissue caecal tonsils and liver and spleen. In both trials, intratracheal delivery of S. Senftenberg was the only route that allowed colonization of the caeca of chickens when compared with oral gavage or intravenous challenge in a dose response fashion (P < 0.05). Liver and spleen samples yielded no S. Seftenberg after the lower dose challenge by the oral or intratracheal route and only low levels following the high-dose administration by these routes, whereas intravenous challenge resulted in recovery of the organisms after both doses. The results of the present study suggest that S. Senftenberg entering the blood is likely to be cleared and will not be able to colonize caeca to the same extent as compared with intratracheal challenge. Clarification of the potential importance of the respiratory tract for transmission of salmonellas under field conditions may be of critical importance to develop intervention strategies to reduce the transmission in poultry. C1 [Kallapura, G.; Morgan, M. J.; Pumford, N. R.; Bielke, L. R.; Wolfenden, A. D.; Faulkner, O. B.; Latorre, J. D.; Menconi, A.; Kuttappan, V. A.; Hargis, B. M.; Tellez, G.] Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. [Kogut, M. H.] USDA ARS, SPARC, College Stn, TX USA. [Hernandez-Velasco, X.] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Mexico City 04510, DF, Mexico. RP Tellez, G (reprint author), Univ Arkansas, Dept Poultry Sci, Fayetteville, AR 72701 USA. EM gtellez@uark.edu OI Latorre, Juan D/0000-0001-6250-566X NR 43 TC 3 Z9 3 U1 1 U2 11 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0307-9457 EI 1465-3338 J9 AVIAN PATHOL JI Avian Pathol. PD AUG PY 2014 VL 43 IS 4 BP 305 EP 309 DI 10.1080/03079457.2014.923554 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AN8PY UT WOS:000340867300004 PM 24820534 ER PT J AU Tingley, MW Wilkerson, RL Bond, ML Howell, CA Siegel, RB AF Tingley, Morgan W. Wilkerson, Robert L. Bond, Monica L. Howell, Christine A. Siegel, Rodney B. TI Variation in home-range size of Black-backed Woodpeckers SO CONDOR LA English DT Article DE Black-backed Woodpecker; burned forest; fire; home range; overlap; Picoides arcticus; snag basal area; space use ID CAVITY-NESTING BIRDS; UTILIZATION DISTRIBUTIONS; PICOIDES-ARCTICUS; CONIFER FORESTS; KERNEL METHODS; BOREAL FOREST; HABITAT USE; SPACE-USE; SELECTION; FIRE AB The Black-backed Woodpecker (Picoides arcticus) is a species of conservation concern that is strongly associated with recently burned forests. Black-backed Woodpeckers are known to have variable home-range sizes, yet the ecological factors related to this variation have not been adequately explored and may hold insights into the natural history of the species and the management of its habitat. During 2011 and 2012, we radio-tracked Black-backed Woodpeckers nesting in 3 forested areas of California that burned between 2 and 5 years before the initiation of tracking. Among 15 individuals with robust tracking data, we found that home-range size varied by an order of magnitude, from 24.1 to 304.1 ha, as measured by movement-based kernel estimation. Using an information-theoretic approach, we evaluated the functional relationship between snag basal area an a priori key resource and home-range size, additionally controlling for sex, age, and years since fire as covariates. We found that snag basal area alone best predicted home-range size, explaining 54-62% of observed variation. As snag basal area increased, home-range sizes exponentially decreased. This relationship held true both with and without the inclusion of 3 individuals that nested in burned forest yet foraged predominantly outside the fire perimeter in unburned forest. Snag basal area, unlike other potential influences on home-range size, is an attribute that forest managers can directly influence. We describe a quantitative relationship between home-range size and snag basal area that forest managers can use to predict Black-backed Woodpecker pair density in burned forests and assess the likely population consequences of specific harvest treatments. Given that the birds in our study, foraging primarily in burned forest, all had home ranges with C1 [Tingley, Morgan W.] Princeton Univ, Woodrow Wilson Sch, Princeton, NJ 08544 USA. [Tingley, Morgan W.; Wilkerson, Robert L.; Bond, Monica L.; Siegel, Rodney B.] Inst Bird Populat, Point Reyes Stn, CA USA. [Howell, Christine A.] US Forest Serv, USDA, Vallejo, CA USA. RP Tingley, MW (reprint author), Princeton Univ, Woodrow Wilson Sch, Princeton, NJ 08544 USA. EM morgan.tingley@uconn.edu OI Tingley, Morgan/0000-0002-1477-2218; Bond, Monica/0000-0001-8500-6564 FU Pacific Southwest Region of the USDA Forest Service; David H. Smith Conservation Research Fellowship FX We thank D. Craig for supporting this project in numerous ways; P. Flebbe for suggesting ways of improving both field logistics and data analysis; D. Yasuda for helping to formulate and refine the project goals; P. Lieske and R. Barnhart for providing information and logistic assistance on Lassen National Forest; C. Dillingham and M. Johnson for providing logistic assistance on Plumas National Forest; R. Taylor at the Institute for Bird Populations (IBP) for assistance with data entry; C. Lehman, J. Tremblay, and J. Dudley for advice on capturing and radio-tagging Black-backed Woodpeckers; and P. Pyle at IBP for inspecting photographs and making final determinations of captured birds' ages. Comments from D. Lee, D. Wilcove, and two anonymous reviewers improved the manuscript and analyses. We are especially grateful to our field technicians for their enthusiasm and hard work: J. Ajani, A. Baz, N. Hagemeyer, J. Leibrecht, M. Lerow, R. Lyon, L. Stinson-Hughes, D. Mauer, S. Shunk, A. Szeitz, E. Trendos, and D. Wolfson. This project was funded by the Pacific Southwest Region of the USDA Forest Service and a David H. Smith Conservation Research Fellowship to M.W.T. and was conducted by the IBP's Sierra Nevada Bird Observatory. This is Contribution no. 466 of the IBP. NR 51 TC 10 Z9 11 U1 5 U2 42 PU COOPER ORNITHOLOGICAL SOC PI LAWRENCE PA ORNITHOLOGICAL SOC NORTH AMER PO BOX 1897, LAWRENCE, KS 66044-8897 USA SN 0010-5422 EI 1938-5129 J9 CONDOR JI Condor PD AUG PY 2014 VL 116 IS 3 BP 325 EP 340 DI 10.1650/CONDOR-13-140.1 PG 16 WC Ornithology SC Zoology GA AO3JD UT WOS:000341224600004 ER PT J AU Comas, LH Callahan, HS Midford, PE AF Comas, Louise H. Callahan, Hilary S. Midford, Peter E. TI Patterns in root traits of woody species hosting arbuscular and ectomycorrhizas: implications for the evolution of belowground strategies SO ECOLOGY AND EVOLUTION LA English DT Article DE Comparative method; K statistic; mycorrhizal colonization; nutrient acquisition strategies; root architecture; root branching intensity; root diameter; root morphology; root tissue density; specific root length ID POTENTIAL GROWTH-RATE; MYCORRHIZAL FUNGI; FLOWERING PLANTS; LAND PLANTS; LIFE-SPAN; BIOGEOCHEMICAL SIGNIFICANCE; INDEPENDENT CONTRASTS; FUNCTIONAL DIVERSITY; TEMPERATE TREES; BRANCH ORDER AB Root traits vary enormously among plant species but we have little understanding of how this variation affects their functioning. Of central interest is how root traits are related to plant resource acquisition strategies from soil. We examined root traits of 33 woody species from northeastern US forests that form two of the most common types of mutualisms with fungi, arbuscular mycorrhizas (AM) and ectomycorrhizas (EM). We examined root trait distribution with respect to plant phylogeny, quantifying the phylogenetic signal (K statistic) in fine root morphology and architecture, and used phylogenetically independent contrasts (PICs) to test whether taxa forming different mycorrhizal associations had different root traits. We found a pattern of species forming roots with thinner diameters as species diversified across time. Given moderate phylogenetic signals (K = 0.44-0.68), we used PICs to examine traits variation among taxa forming AM or EM, revealing that hosts of AM were associated with lower branching intensity (r(PIC) = -0.77) and thicker root diameter (rPIC = -0.41). Because EM evolved relatively more recently and intermittently across plant phylogenies, significant differences in root traits and colonization between plants forming AM and EM imply linkages between the evolution of these biotic interactions and root traits and suggest a history of selection pressures, with trade-offs for supporting different types of associations. Finally, across plant hosts of both EM and AM, species with thinner root diameters and longer specific root length (SRL) had less colonization (rPIC = 0.85, -0.87), suggesting constraints on colonization linked to the evolution of root morphology. C1 [Comas, Louise H.] Penn State Univ, Dept Hort, Intercoll Grad Program Ecol, University Pk, PA 16802 USA. [Comas, Louise H.] ARS, USDA, Water Management Res Unit, Ft Collins, CO 80526 USA. [Callahan, Hilary S.] Columbia Univ, Dept Biol Sci, Barnard Coll, New York, NY 10027 USA. [Midford, Peter E.] NESCent, Durham, NC 27705 USA. RP Comas, LH (reprint author), ARS, USDA, Water Management Res Unit, 2150 Ctr Ave,Bldg D Suite 320, Ft Collins, CO 80526 USA. EM louise.comas@ars.usda.gov OI Midford, Peter/0000-0001-6512-3296 FU MORPH-RCN; NSF [IOS-0719259]; NESCent FX We are particularly indebted to J. Harding, M. Munson, and W. Schuster for help identifying field sites; J. Baldwin, B. Carlisle Carter, S. Leicht, and A. Patterson for assistance with data collection and sparking discussion; D. Eissenstat, L. Jackson, and B. Lampinen for providing departmental housing to LHC; and D. Flynn and K. McGuire for comments that improved this manuscript. This study was funded by MORPH-RCN, NSF IOS-0719259, and NESCent. NR 77 TC 12 Z9 12 U1 14 U2 93 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD AUG PY 2014 VL 4 IS 15 BP 2979 EP 2990 DI 10.1002/ece3.1147 PG 12 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA AN4RI UT WOS:000340575000001 PM 25247056 ER PT J AU Elder, T Beste, A AF Elder, Thomas Beste, Ariana TI Density Functional Theory Study of the Concerted Pyrolysis Mechanism for Lignin Models SO ENERGY & FUELS LA English DT Article ID BOND-DISSOCIATION ENTHALPIES; PHENETHYL PHENYL ETHER; LOW-TEMPERATURE THERMOLYSIS; ALPHA/BETA-SELECTIVITIES; COMPUTATIONAL PREDICTION; THERMAL-DEGRADATION; COMPOUND; DEPOLYMERIZATION; CHEMISTRY; LINKAGES AB Studies on the pyrolysis mechanisms of lignin model compounds have largely focused on initial homolytic cleavage reactions. It has been noted, however, that concerted mechanisms may also account for observed product formation. In the current work, the latter processes are examined and compared to the former, by the application of density functional theory calculations to fully substituted lignin models. Results show that activation energies for the concerted reactions are somewhat lower than the bond dissociation energies of the homolysis reactions. Kinetic analysis revealed that the concerted pathway is the retro-ene fragmentation mechanism. C1 [Elder, Thomas] Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. [Beste, Ariana] Univ Tennessee, Joint Inst Computat Sci, Knoxville, TN 37996 USA. RP Elder, T (reprint author), Forest Serv, Southern Res Stn, USDA, Pineville, LA 71360 USA. EM telder@fs.fed.us NR 43 TC 13 Z9 14 U1 3 U2 47 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0887-0624 EI 1520-5029 J9 ENERG FUEL JI Energy Fuels PD AUG PY 2014 VL 28 IS 8 BP 5229 EP 5235 DI 10.1021/ef5013648 PG 7 WC Energy & Fuels; Engineering, Chemical SC Energy & Fuels; Engineering GA AN7VJ UT WOS:000340808800044 ER PT J AU Li, M Sheng, GP Wu, YJ Yu, ZL Banuelos, GS Yu, HQ AF Li, Miao Sheng, Guo-ping Wu, Yue-jin Yu, Zeng-liang Banuelos, Gary S. Yu, Han-qing TI Enhancement of nitrogen and phosphorus removal from eutrophic water by economic plant annual ryegrass (Lolium multiflorum) with ion implantation SO ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH LA English DT Article DE Eutrophication; Phytoremediation; Ion implantation; Economic plants; Ryegrass; Lolium multiflorum ID DOMESTIC WASTE-WATER; PHYTOREMEDIATION; TERRESTRIAL; WASTEWATERS; SYSTEM AB Severe eutrophication of surface water has been a major problem of increasing environmental concern worldwide. In the present study, economic plant annual ryegrass (Lolium multiflorum) was grown in floating mats as an economic plant-based treatment system to evaluate its potential after ion implantation for removing nutrients in simulated eutrophic water. The specific weight growth rate of L. multiflorum with ion implantation was significantly greater than that of the control, and the peroxidase, nitrate reductase, and acid phosphatase activities of the irradiated L. multiflorum were found to be greater than those plants without ion implantation. Higher total nitrogen (TN) and total phosphorus (TP) removal efficiencies were obtained for the L. multiflorum irradiated with 25 keV 5.2 x 10(16) N+ ions/cm(2) and 30 keV 4.16 x 10(16) N+ ions/cm(2), respectively (p < 0.05). Furthermore, the nitrogen and phosphorus contents in the plant biomass with ion implantation were also greater than those in the control and were positively correlated with TN and TP supplied. L. multiflorum itself was directly responsible for 39-49 and 47-58 % of the overall N and P removal in the experiment, respectively. The research results suggested that ion implantation could become a promising approach for increasing phytoremediation efficiency of nutrients from eutrophic water by L. multiflorum. C1 [Li, Miao] Anhui Agr Univ, Sch Resources & Environm & Plant Protect, Key Lab Agri Food Safety Anhui Prov, Hefei 230036, Peoples R China. [Li, Miao; Sheng, Guo-ping; Yu, Han-qing] Univ Sci & Technol China, Sch Chem, Environm Engn Lab, Hefei 230026, Peoples R China. [Li, Miao; Wu, Yue-jin; Yu, Zeng-liang] Chinese Acad Sci, Inst Plasma Phys, Key Lab Ion Beam Bioengn, Hefei 230031, Peoples R China. [Banuelos, Gary S.] USDA ARS, Parlier, CA 93648 USA. RP Yu, HQ (reprint author), Univ Sci & Technol China, Sch Chem, Environm Engn Lab, Hefei 230026, Peoples R China. EM miaoli@ustc.edu.cn; hqyu@ustc.edu.cn RI Yu, Han-Qing/F-7925-2010; Sheng, Guo-Ping/F-2074-2010 FU National Natural Science Foundation of China [50949038]; China Postdoctoral Science Foundation [20060400722]; National Hi-technology Development 863 Program of China [2006AA06Z340] FX The authors wish to thank Drs. Lixiang Yu and Hang Yuan (Institute of Plasma Physics, Chinese Academy of Sciences) for conducting their ion implantation. We also thank Dr. Zhiqing Lin who comes from Southern Illinois University Edwardsville for his useful comments and suggestions on the language and structure of our manuscript. The work was partially supported by the National Natural Science Foundation of China (50949038), China Postdoctoral Science Foundation (20060400722), National Hi-technology Development 863 Program of China (2006AA06Z340). NR 26 TC 5 Z9 5 U1 1 U2 47 PU SPRINGER HEIDELBERG PI HEIDELBERG PA TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY SN 0944-1344 EI 1614-7499 J9 ENVIRON SCI POLLUT R JI Environ. Sci. Pollut. Res. PD AUG PY 2014 VL 21 IS 16 BP 9617 EP 9625 DI 10.1007/s11356-014-2987-4 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AN5EO UT WOS:000340613200020 PM 24809498 ER PT J AU Harris, RA Nagy-Szakal, D Mir, SAV Frank, E Szigeti, R Kaplan, JL Bronsky, J Opekun, A Ferry, GD Winter, H Kellermayer, R AF Harris, R. Alan Nagy-Szakal, Dorottya Mir, Sabina A. V. Frank, Eibe Szigeti, Reka Kaplan, Jess L. Bronsky, Jiri Opekun, Antone Ferry, George D. Winter, Harland Kellermayer, Richard TI DNA methylation-associated colonic mucosal immune and defense responses in treatment-naive pediatric ulcerative colitis SO EPIGENETICS LA English DT Article DE Pediatric inflammatory bowel disease; ulcerative colitis; treatment-naive; epigenetics; DNA methylation; DNA expression; DNA methylome ID INFLAMMATORY-BOWEL-DISEASE; CROHNS-DISEASE; GENE-EXPRESSION; PREVALENCE; ONSET; SUSCEPTIBILITY; IDENTIFICATION; EPIDEMIOLOGY; MICROARRAYS; VALIDATION AB Inflammatory bowel diseases (IBD) are emerging globally, indicating that environmental factors may be important in their pathogenesis. Colonic mucosal epigenetic changes, such as DNA methylation, can occur in response to the environment and have been implicated in IBD pathology. However, mucosal DNA methylation has not been examined in treatment-naive patients. We studied DNA methylation in untreated, left sided colonic biopsy specimens using the Infinium HumanMethylation450 BeadChip array. We analyzed 22 control (C) patients, 15 untreated Crohn's disease (CD) patients, and 9 untreated ulcerative colitis (UC) patients from two cohorts. Samples obtained at the time of clinical remission from two of the treatment-naive UC patients were also included into the analysis. UC-specific gene expression was interrogated in a subset of adjacent samples (5 C and 5 UC) using the Affymetrix GeneChip PrimeView Human Gene Expression Arrays. Only treatment-naive UC separated from control. One-hundred-and-20 genes with significant expression change in UC (>2-fold, P < 0.05) were associated with differentially methylated regions (DMRs). Epigenetically associated gene expression changes (including gene expression changes in the IFITM1, ITGB2, S100A9, SLPI, SAA1, and STAT3 genes) were linked to colonic mucosal immune and defense responses. These findings underscore the relationship between epigenetic changes and inflammation in pediatric treatment-naive UC and may have potential etiologic, diagnostic, and therapeutic relevance for IBD. C1 [Harris, R. Alan] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. [Nagy-Szakal, Dorottya; Mir, Sabina A. V.; Ferry, George D.; Kellermayer, Richard] Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr,Texas Childrens Hosp, Houston, TX 77030 USA. [Frank, Eibe] Univ Waikato, Dept Comp Sci, Hamilton, New Zealand. [Szigeti, Reka] Baylor Coll Med, Dept Pathol, Houston, TX 77030 USA. [Kaplan, Jess L.; Winter, Harland] MassGen Hosp Children, Dept Pediat, Boston, MA USA. [Bronsky, Jiri] Charles Univ Prague, Dept Pediat, Prague, Czech Republic. [Bronsky, Jiri] Univ Hosp Motol, Prague, Czech Republic. [Opekun, Antone] Baylor Coll Med, Dept Gastroenterol, Houston, TX 77030 USA. RP Kellermayer, R (reprint author), Baylor Coll Med, Dept Pediat, USDA, ARS,Childrens Nutr Res Ctr,Texas Childrens Hosp, Houston, TX 77030 USA. EM kellerma@bcm.edu FU Broad Medical Research Program, the Broad Foundation [IBD-0252]; Child Health Research Career Development Agency of the Baylor College of Medicine (NIH) [5K12 HD041648]; Public Health Service grant [DK56338]; Karen and Brock Wagner family; Pediatric Inflammatory Bowel Disease Foundation FX R. K. was supported in part by the Broad Medical Research Program, the Broad Foundation (IBD-0252); the Child Health Research Career Development Agency of the Baylor College of Medicine (NIH # 5K12 HD041648); a Public Health Service grant DK56338, funding the Texas Medical Center Digestive Diseases Center; and the Gutsy Kids Fund including philanthropic donation from the Karen and Brock Wagner family. H. S. W. was supported in part by a philanthropic donation from the Martin Schlaff Family. This project was also supported by the Pediatric Inflammatory Bowel Disease Foundation. NR 42 TC 12 Z9 13 U1 3 U2 9 PU LANDES BIOSCIENCE PI AUSTIN PA 1806 RIO GRANDE ST, AUSTIN, TX 78702 USA SN 1559-2294 EI 1559-2308 J9 EPIGENETICS-US JI Epigenetics PD AUG PY 2014 VL 9 IS 8 BP 1131 EP 1137 DI 10.4161/epi.29446 PG 7 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA AO5CV UT WOS:000341360600008 PM 24937444 ER PT J AU Hoberg, EP Phillips, AJ AF Hoberg, Eric P. Phillips, Anna J. TI Transfer of the US-National Parasite Collection SO FOLIA PARASITOLOGICA LA English DT Editorial Material C1 [Hoberg, Eric P.] ARS, US Natl Parasite Collect, USDA, Beltsville, MD USA. [Phillips, Anna J.] Smithsonians Natl Museum Nat Hist, Dept Invertebrate Zool, Washington, DC USA. RP Phillips, AJ (reprint author), Smithsonian Inst, Natl Museum Nat Hist, Dept Invertebrate Zool, Washington, DC 20560 USA. EM phillipsaj@si.edu NR 4 TC 0 Z9 0 U1 0 U2 0 PU FOLIA PARASITOLOGICA PI CESKE BUDEJOVICE PA BRANISOVSKA 31,, CESKE BUDEJOVICE 370 05, CZECH REPUBLIC SN 0015-5683 EI 1803-6465 J9 FOLIA PARASIT JI Folia Parasitol. PD AUG PY 2014 VL 61 IS 4 BP 370 EP 370 PG 1 WC Parasitology SC Parasitology GA AN4SC UT WOS:000340577800009 PM 25185407 ER PT J AU Blackburn, H Plante, Y AF Blackburn, H. Plante, Y. TI North American animal breeding and production: meeting the needs of a changing landscape SO JOURNAL OF ANIMAL BREEDING AND GENETICS LA English DT Editorial Material C1 [Blackburn, H.] ARS, USDA, Ft Collins, CO 80521 USA. [Plante, Y.] Agr & Agri Food Canada, Saskatoon, SK, Canada. RP Blackburn, H (reprint author), ARS, USDA, Ft Collins, CO 80521 USA. EM Harvey.Blackburn@ars.usda.gov NR 0 TC 1 Z9 1 U1 1 U2 3 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0931-2668 EI 1439-0388 J9 J ANIM BREED GENET JI J. Anim. Breed. Genet. PD AUG PY 2014 VL 131 IS 4 BP 247 EP 248 DI 10.1111/jbg.12098 PG 2 WC Agriculture, Dairy & Animal Science SC Agriculture GA AN5XX UT WOS:000340667200001 PM 24943115 ER PT J AU Avolio, F Rimando, AM Cimmino, A Andolfi, A Jain, S Tekwani, BL Evidente, A AF Avolio, Fabiana Rimando, Agnes M. Cimmino, Alessio Andolfi, Anna Jain, Surendra Tekwani, Babu L. Evidente, Antonio TI Inuloxins A-D and derivatives as antileishmanial agents: structure-activity relationship study SO JOURNAL OF ANTIBIOTICS LA English DT Article ID CUTANEOUS LEISHMANIASIS; SESQUITERPENE LACTONES C1 [Avolio, Fabiana; Cimmino, Alessio; Andolfi, Anna; Evidente, Antonio] Univ Naples Federico II, Dipartimento Sci Chim, I-480126 Naples, Italy. [Avolio, Fabiana; Rimando, Agnes M.] Univ Mississippi, Nat Prod Utilizat Res Unit, ARS, USDA, University, MS 38677 USA. [Jain, Surendra; Tekwani, Babu L.] Univ Mississippi, Sch Pharm, Pharmaceut Sci Res Inst, Natl Ctr Nat Prod Res, University, MS 38677 USA. [Tekwani, Babu L.] Univ Mississippi, Sch Pharm, Dept Pharmacol, University, MS 38677 USA. RP Evidente, A (reprint author), Univ Naples Federico II, Dipartimento Sci Chim, Complesso Univ Monte S Angelo,Via Cintia 4, I-480126 Naples, Italy. EM evidente@unina.it RI Evidente, Antonio/N-9357-2013; Cimmino, Alessio/I-2121-2012 OI Evidente, Antonio/0000-0001-9110-1656; Cimmino, Alessio/0000-0002-1551-4237 FU ARIMNET subprogram MEDILEG from the European Union; US Department of Defense CDMRP [W81XWH-09-2-0093]; USDA ARS [58-6408-1-603] FX We thank Dr Maurizio Vurro, Instituto di Scienze delle Produzioni Alimentari, CNR, Bari, Italy for providing the plant material. We thank Dr Melissa Jacob for the general screening for antimicrobial activity of the compounds. We also thank Ms Gloria Hervey for technical help. This research was supported in part by ARIMNET subprogram MEDILEG from the European Union, Call 2011. Dr Babu L. Tekwani is supported by US Department of Defense CDMRP, grant No. W81XWH-09-2-0093 and USDA ARS Specific Cooperative Agreement No. 58-6408-1-603. Professor A. Evidente is also associated with the Instituto di Chimica Biomolecolare, CNR, Pozzuoli, Italy. NR 15 TC 3 Z9 3 U1 2 U2 4 PU JAPAN ANTIBIOTICS RESEARCH ASSOC PI TOKYO PA 2 20 8 KAMIOSAKI SHINAGAWA KU, TOKYO, 141, JAPAN SN 0021-8820 J9 J ANTIBIOT JI J. Antibiot. PD AUG PY 2014 VL 67 IS 8 BP 597 EP 601 DI 10.1038/ja.2014.47 PG 5 WC Biotechnology & Applied Microbiology; Immunology; Microbiology; Pharmacology & Pharmacy SC Biotechnology & Applied Microbiology; Immunology; Microbiology; Pharmacology & Pharmacy GA AO1LW UT WOS:000341074600011 PM 24781956 ER PT J AU Treumann, S Torkzaban, S Bradford, SA Visalakshan, RM Page, D AF Treumann, Svantje Torkzaban, Saeed Bradford, Scott A. Visalakshan, Rahul M. Page, Declan TI An explanation for differences in the process of colloid adsorption in batch and column studies SO JOURNAL OF CONTAMINANT HYDROLOGY LA English DT Article DE Colloid; Bacteria; Batch; PARP-1; Column experiments; Porous media; Attachment ID SATURATED POROUS-MEDIA; UNFAVORABLE DEPOSITION CONDITIONS; MICROMETER-SCALE PARTICLES; WATER-SOLID INTERFACE; SOLUTION CHEMISTRY; SURFACE-ROUGHNESS; RATE COEFFICIENTS; IONIC-STRENGTH; SAND COLUMNS; TRANSPORT AB It is essential to understand the mechanisms that control virus and bacteria removal in the subsurface environment to assess the risk of groundwater contamination with fecal microorganisms. This study was conducted to explicitly provide a critical and systematic comparison between batch and column experiments. The aim was to investigate the underlying factors causing the commonly observed discrepancies in colloid adsorption process in column and batch systems. We examined the colloid adsorption behavior of four different sizes of carboxylate-modified latex (CML) microspheres, as surrogates for viruses and bacteria, on quartz sand in batch and column experiments over a wide range of solution ionic strengths (IS). Our results show that adsorption of colloids in batch systems should be considered as an irreversible attachment because the attachment/detachment model was found to be inadequate in describing the batch results. An irreversible attachment-blocking model was found to accurately describe the results of both batch and column experiments. The rate of attachment was found to depend highly on colloid size, solution IS and the fraction of the sand surface area favorable for attachment (S-f). The rate of attachment and S-f values were different in batch and column experiments due to differences in the hydrodynamic of the system, and the role of surface roughness and pore structure on colloid attachment. Results from column and batch experiments were generally not comparable, especially for larger colloids (>= 0.5 mu m). Predictions based on classical DLVO theory were found to inadequately describe interaction energies between colloids and sand surfaces. (C) 2014 Elsevier B.V. All rights reserved. C1 [Treumann, Svantje; Torkzaban, Saeed; Visalakshan, Rahul M.; Page, Declan] CSIRO, Land & Water, Glen Osmond, SA 5064, Australia. [Bradford, Scott A.] ARS, USDA, Salin Lab, Riverside, CA 92507 USA. RP Torkzaban, S (reprint author), CSIRO, Land & Water, Glen Osmond, SA 5064, Australia. EM Saeed.Torkzaban@csiro.au RI Torkzaban, Saeed/G-7377-2013; Page, Declan/F-2566-2011 OI Torkzaban, Saeed/0000-0002-5146-9461; Page, Declan/0000-0002-4902-3911 FU Gas Industry Social and Environmental Research Alliance (GISERA) FX This work was supported by the Gas Industry Social and Environmental Research Alliance (GISERA). GISERA is a collaborative vehicle established by CSIRO and Australia Pacific LNG to undertake publicly reported research addressing the socio-economic and environmental impacts of Australia's natural gas industries. For more details about GISERA visit www.gisera.org.au. We would like to thank Mike Donn from CSIRO Land and Water for his comment that helped to strengthen the article. NR 64 TC 21 Z9 21 U1 8 U2 52 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0169-7722 EI 1873-6009 J9 J CONTAM HYDROL JI J. Contam. Hydrol. PD AUG PY 2014 VL 164 BP 219 EP 229 DI 10.1016/j.jconhyd.2014.06.007 PG 11 WC Environmental Sciences; Geosciences, Multidisciplinary; Water Resources SC Environmental Sciences & Ecology; Geology; Water Resources GA AO0HG UT WOS:000340989100018 PM 24997430 ER PT J AU Paiva, FF Vanier, NL Berrios, JD Pan, J Villanova, FD Takeoka, G Elias, MC AF Paiva, Flavia Fernandes Vanier, Nathan Levien Berrios, Jose De Jesus Pan, James Villanova, Franciene de Almeida Takeoka, Gary Elias, Moacir Cardoso TI Physicochemical and nutritional properties of pigmented rice subjected to different degrees of milling SO JOURNAL OF FOOD COMPOSITION AND ANALYSIS LA English DT Article DE Oryza sativa L.; Colour; Bran removal; Antioxidants; Anthocyanins; Proanthocyanidins; Food analysis; Food composition ID ANTIOXIDANT ACTIVITY; PHENOLIC-COMPOUNDS; VARIETIES; ANTHOCYANINS; PROFILES; CAPACITY; PROANTHOCYANIDINS; PHYTOCHEMICALS; QUALITY; ACIDS AB Recent studies have reported the health benefits of pigmented rice cultivars due to the presence of bioactive compounds in the bran layer of caryopsis. This study evaluated the proximate composition, colour, total flavonoids, anthocyanins and proanthocyanidins contents, as well as the total phenolics and antioxidant activity of IAC-600 black rice cultivar and MPB-10 red rice lineage, as a function of degree of milling (DOM), at 0%, 4%, 7%, 10%, 12% and 15% of DOM. Black rice showed a thicker bran layer than red rice. Around 80% and 65% of the ash content of red and black rice, respectively, was distributed in the bran layer. 4% DOM reduced 47% of the fat content in red rice, while in order to reduce similar fat content in black rice, a 7% DOM was necessary. The total free phenolics were around 6- and 7-fold higher than bound phenolics for black and red rice, respectively. Although the non-milled black rice presented higher free and bound phenolics contents than non-milled red rice, the red rice showed higher DPPH and ABTS(center dot+) antioxidant activities. (C) 2014 Elsevier Inc. All rights reserved. C1 [Paiva, Flavia Fernandes; Vanier, Nathan Levien; Villanova, Franciene de Almeida; Elias, Moacir Cardoso] Univ Fed Pelotas, Dept Agroind Sci & Technol, BR-96010900 Pelotas, Brazil. [Paiva, Flavia Fernandes; Vanier, Nathan Levien; Berrios, Jose De Jesus; Pan, James; Takeoka, Gary] USDA, Proc Foods Res Unit, WRRC, ARS, Albany, CA 94710 USA. RP Vanier, NL (reprint author), Univ Fed Pelotas, Dept Agroind Sci & Technol, BR-96010900 Pelotas, Brazil. EM nathanvanier@hotmail.com OI Vanier, Nathan/0000-0001-6592-3023 NR 32 TC 11 Z9 14 U1 5 U2 35 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0889-1575 EI 1096-0481 J9 J FOOD COMPOS ANAL JI J. Food Compos. Anal. PD AUG PY 2014 VL 35 IS 1 BP 10 EP 17 DI 10.1016/j.jfca.2014.05.003 PG 8 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AO0DG UT WOS:000340978700002 ER PT J AU Matthan, NR Ooi, EM Van Horn, L Neuhouser, ML Woodman, R Lichtenstein, AH AF Matthan, Nirupa R. Ooi, Esther M. Van Horn, Linda Neuhouser, Marian L. Woodman, Richard Lichtenstein, Alice H. TI Plasma Phospholipid Fatty Acid Biomarkers of Dietary Fat Quality and Endogenous Metabolism Predict Coronary Heart Disease Risk: A Nested Case-Control Study Within the Women's Health Initiative Observational Study SO JOURNAL OF THE AMERICAN HEART ASSOCIATION LA English DT Article DE diet; fatty acids; risk factors; women ID MIDDLE-AGED ADULTS; LONG-CHAIN OMEGA-3-FATTY-ACIDS; NONFATAL MYOCARDIAL-INFARCTION; CARDIOVASCULAR HEALTH; ADIPOSE-TISSUE; ATHEROSCLEROSIS RISK; OLDER-ADULTS; DESATURASE ACTIVITY; MILK-FAT; DOCOSAHEXAENOIC ACID AB Background-Although the relationship between dietary fat quality and coronary heart disease (CHD) risk has been evaluated, typically using diet questionnaires, results are inconsistent and data in postmenopausal women are limited. Plasma phospholipid fatty acid (PL-FA) profiles, reflecting dietary intake and endogenous FA metabolism, may better predict diet-CHD risk. Methods and Results-Using a nested case-control design, we assessed the association between plasma PL-FA profiles and CHD risk in 2448 postmenopausal women (1224 cases with confirmed CHD and 1224 controls matched for age, enrollment date, race/ethnicity, and absence of CHD at baseline and after 4.5 years of follow-up) participating in the Women's Health Initiative observational study. PL-FA profile was measured using gas chromatography. Product/precursor ratios were used to estimate stearoyl-CoA-desaturase (16:1n-7/16: 0, 18:1n-9/18: 0), Delta 6-desaturase (20:3n-6/18:2n-6), and Delta 5-desaturase (20:4n-6/20:3n-6) activities, indicators of endogenous FA metabolism. Multivariate conditional logistic regression was used to obtain odds ratios (95% CIs) for CHD risk. While no associations were observed for the predominant PL fatty acid (16: 0, 18: 0, 18: 1n-9, and 18: 2n-6), plasma PL-saturated fatty acid (1.20 [1.08 to 1.32]) and endogenously synthesized PL omega 6 fatty acids (20: 3n-6; 3.22 [1.95 to 5.32]), 22: 5n-6; 1.63 [1.20 to 2.23]) and Delta 6-desaturase (1.25 [1.11 to 1.41]) were positively associated with CHD risk. PL-omega 3 fatty acids (20: 5n-3; 0.73 [0.58 to 0.93], 22: 5n-3; 0.56 [0.33 to 0.94], 22: 6n-3; 0.56 [0.39 to 0.80]), 18: 1n-7 (0.54 [0.29 to 0.99]), and Delta 5-desaturase (0.78 [0.70 to 0.88]) were inversely associated with CHD risk. Results support current guidelines regarding regular fish consumption. Additional findings include associations between endogenously synthesized fatty acids and CHD risk, which were partly explained by changes in Delta 6-desaturase and Delta 5-desaturase indexes, suggesting that in vivo metabolism may also play an important role in predicting CHD risk in this cohort of postmenopausal women. C1 [Matthan, Nirupa R.; Lichtenstein, Alice H.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Ooi, Esther M.] Univ Western Australia, Sch Med & Pharmacol, Perth, WA 6009, Australia. [Van Horn, Linda] Northwestern Univ, Chicago, IL 60611 USA. [Neuhouser, Marian L.] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA. [Woodman, Richard] Flinders Univ S Australia, Sch Med, Bedford Pk, SA 5042, Australia. RP Matthan, NR (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM nirupa.matthan@tufts.edu FU National Heart, Lung, and Blood Institute, National Institutes of Health; US Department of Health and Human Services [HHSN26800764317C]; US Department of Health and Human Services through contract ADB [N01-WH-74317, HHSN268201100046C, HHSN2682011 00001C, HHSN268201100002C, HHSN268201100003C]; National Health and Medical Research Council (NHMRC) of Australia Postdoctoral Research Fellowship FX We are grateful to the WHI participants, staff, and investigators. Program Office: Jacques Rossouw, Shari Ludlam, JoanMcGowan, Leslie Ford, and Nancy Geller. Clinical Coordinating Center: Ross Prentice, Garnet Anderson, Andrea LaCroix, Charles Kooperberg, Evan Stein, and Steven Cummings. Clinical Centers: Sylvia Wasstertheil-Smoller, Haleh Sangi-Haghpeykar, JoAnn Manson, Charles Eaton, Lawrence Phillips, Shirley Beresford, Lisa Martin, Rowan Chlebowski, Erin LeBlanc, Bette Caan, Jane Morley Kotchen, Barbara Howard, Linda Van Horn, Henry Black, Marcia Stefanick, Dorothy Lane, Rebecca Jackson, Cora Lewis, Cynthia Thomson, Jean Wactawski-Wende, John Robbins, F. Allan Hubbell, Lauren Nathan, Robert Langer, Marian Limacher, J. David Curb, Robert Wallace, Judith Ockene, Norman Lasser, Mary Jo O'Sullivan, Karen Margolis, Robert Brunner, Gerardo Heiss, Lewis Kuller, Karen Johnson, Robert Bryzski, Gloria Sarto, Mara Vitolins, and Michael Simon. This work was supported by the National Heart, Lung, and Blood Institute, National Institutes of Health, and US Department of Health and Human Services through contracts HHSN26800764317C, and ADB contract N01-WH-74317, HHSN268201100046C, HHSN2682011 00001C, HHSN268201100002C, and HHSN268201100003C. Dr Ooi is supported by a National Health and Medical Research Council (NHMRC) of Australia Postdoctoral Research Fellowship. NR 69 TC 11 Z9 11 U1 2 U2 8 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2047-9980 J9 J AM HEART ASSOC JI J. Am. Heart Assoc. PD AUG PY 2014 VL 3 IS 4 AR e000764 DI 10.1161/JAHA.113.000764 PG 10 WC Cardiac & Cardiovascular Systems SC Cardiovascular System & Cardiology GA AO4HD UT WOS:000341296600010 ER PT J AU Silva, MVB dos Santos, DJA Boison, SA Utsunomiya, ATH Carmo, AS Sonstegard, TS Cole, JB Van Tassell, CP AF Silva, Marcos V. B. dos Santos, Daniel J. A. Boison, Solomon A. Utsunomiya, Adam T. H. Carmo, Adriana S. Sonstegard, Tad S. Cole, John B. Van Tassell, Curt P. TI The development of genomics applied to dairy breeding SO LIVESTOCK SCIENCE LA English DT Article DE Dairy cattle; Genomics; SNP; Genomic selection ID SINGLE-NUCLEOTIDE POLYMORPHISMS; MARKER-ASSISTED SELECTION; HOLSTEIN-FRIESIAN CATTLE; LINKAGE DISEQUILIBRIUM; UNITED-STATES; SNP GENOTYPES; GENETIC EVALUATIONS; WIDE SELECTION; DATA SETS; POPULATION AB Genomic selection (GS) has profoundly changed dairy cattle breeding in the last decade and can be defined as the use of genomic breeding values (GEBV) in selection programs. The GEBV is the sum of the effects of dense DNA markers across the whole genome, capturing all the quantitative trait loci (QTL) that contribute to variation in a trait. This technology was successfully implemented in the United States, Canada, New Zealand, Australia, and several European countries with very promising results. The GEBV reliability depends on estimation procedures and models. The different methodologies to estimate SNP effects and GEBV have been extensively tested for many research groups with very promising results. Although GS is a success, many challenges still remain, including integration of GEBV into genetic evaluation programs and increasing GEBV reliability. The aim of this review is to discuss the main aspects involved with GS, including different methodologies of imputation, SNP effect estimation, and the most important impacts of GS implementation in dairy cattle. (C) 2014 Elsevier B.V. All rights reserved. C1 [Silva, Marcos V. B.; Carmo, Adriana S.] Empresa Brasileira Pesquisa Agr, Embrapa Gado Leite, BR-36038330 Juiz De Fora, MG, Brazil. [dos Santos, Daniel J. A.; Utsunomiya, Adam T. H.] Univ Estadual Paulista UNESP, BR-14884900 Jaboticabal, SP, Brazil. [Boison, Solomon A.] Univ Nat Resources & Life Sci, A-1180 Vienna, Austria. [Sonstegard, Tad S.; Cole, John B.; Van Tassell, Curt P.] USDA, ARS, Anim Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Silva, MVB (reprint author), Empresa Brasileira Pesquisa Agr, Embrapa Gado Leite, Rua Eugenio Nascimento 610 Dom Bosco, BR-36038330 Juiz De Fora, MG, Brazil. EM marcos.vb.silva@embrapa.br; daniel.jordan2008@hotmail.com; soloboan@yahoo.com; adamtaiti@gmail.com; adriana.carmo@colaborador.embrapa.br; tad.sonstegard@ars.usda.gov; john.cole@ars.usda.gov; curt.vantassell@ars.usda.gov RI Carmo, Andreia/D-8603-2012; Utsunomiya, Adam/P-3624-2015; Cole, John/J-8571-2014 OI Utsunomiya, Adam/0000-0002-5362-4832; Cole, John/0000-0003-1242-4401 FU Embrapa - SEG [02.09.07.008.00.00]; CNPq [PVE 407246/2013-4]; FAPEMIG [CVZ PPM 00325/12]; USDA Agricultural Research Service [1245-31000-101-00, 1245-31000-104-00] FX Marcos V. Silva was supported by the Embrapa - SEG 02.09.07.008.00.00 "Genomic Selection in Dairy Cattle in Brazil", CNPq PVE 407246/2013-4 "Genomic Selection in Dairy Gir and Girolando Breeds", and FAPEMIG CVZ PPM 00325/12 "SNP Discovery in Brazilian Dairy Zebu Cattle" appropriated projects. John B. Cole was supported by the USDA Agricultural Research Service appropriated project 1245-31000-101-00, "Improving GeneticPredictions in Dairy Animals Using Phenotypic and Genomic Information." Tad S. Sonstegard and Curt P. Van Tassell were supported by USDA Agricultural Research Service appropriated projects 1245-31000-101-00, "Improving Genetic Predictions in Dairy Animals Using Phenotypic and Genomic Information", and 1245-31000-104-00, "Enhancing Genetic Merit of Ruminants Through Genome Selection and Analysis." The USDA is an equal opportunity provider and employer. NR 117 TC 2 Z9 2 U1 2 U2 23 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD AUG PY 2014 VL 166 SI SI BP 66 EP 75 DI 10.1016/j.livsci.2014.05.017 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA AO0JD UT WOS:000340994000010 ER PT J AU O'Brien, AMP Meszaros, G Utsunomiya, YT Sonstegard, TS Garcia, JF Van Tassell, CP Carvalheiro, R da Silva, MVB Solkner, J AF O'Brien, Ana M. Perez Meszaros, Gabor Utsunomiya, Yuri T. Sonstegard, Tad S. Garcia, J. Fernando Van Tassell, Curtis P. Carvalheiro, Roberto da Silva, Marcos V. B. Solkner, Johann TI Linkage disequilibrium levels in Bos indicus and Bos taurus cattle using medium and high density SNP chip data and different minor allele frequency distributions SO LIVESTOCK SCIENCE LA English DT Article DE Linkage disequilibrium; Taurine; Indicine; Cattle; Minor allele frequency ID EFFECTIVE POPULATION-SIZE; HAPLOTYPE BLOCK STRUCTURE; WHOLE-GENOME ASSOCIATION; HOLSTEIN-FRIESIAN CATTLE; WIDE ASSOCIATION; DAIRY-CATTLE; BREEDING VALUES; BEEF-CATTLE; GENOTYPES; EXTENT AB Linkage disequilibrium (LD), the observed correlation between alleles at different loci in the genome, is a determinant parameter in many applications of molecular genetics. With the wider use of genomic technologies in animal breeding and animal genetics, it is worthwhile revising and improving the current knowledge and understanding of cattle LD. This study analyzes levels of ID assessed through the r(2) measurement in seven breeds of cattle from both indicine (Bos indicus) and taurine (Bos taurus) (sub)species, genotyped with a high density panel (HD) of over 777,000 single nucleotide polymorphism (SNP) markers. Average levels of LD were assessed for inter-marker distances from 0 kb up to 10 Mb. Two more SNP panels were built in silico from the original genotypes to represent a lower density SNP chip including approximately 50,000 SNPs (50K) and a panel of 249,000 SNPs chosen to comply with specific minor allele frequency (MAF) distributions. The results show higher levels of ID than previously reported, especially at short inter-marker distances of less than 20 kb, assessed through the use of the higher density panel. Negligible impact of MAF changes in the estimation of r(2) was observed, supporting the use of the HD panel for LD-based methodologies even when ascertainment bias in the choice of SNPs is suspected. The 50K SNP panel indicated lower levels of LD in the first 100 kb and highly unreliable LD estimates for distances shorter than 50 kb. Overall, lower levels of LD were observed for all indicine breeds as compared to taurine breeds, with larger differences assessed in the first 100 kb, suggesting the need for use of higher SNP densities in this (sub) species when applying LD-dependent methods, as compared to most taurine breeds. (C) 2014 Elsevier B.V. All rights reserved. C1 [O'Brien, Ana M. Perez; Meszaros, Gabor; Solkner, Johann] Univ Nat Resources & Life Sci BOKU, Dept Sustainable Agr Syst, A-1180 Vienna, Austria. [Utsunomiya, Yuri T.; Garcia, J. Fernando; Carvalheiro, Roberto] Univ Estadual Paulista, UNESP, Fac Ciencias Agr Vet, Jaboticabal, SP, Brazil. [Sonstegard, Tad S.; Van Tassell, Curtis P.] USDA, Agr Res Serv, Bovine Funct Genom Lab, Beltsville, MD 20705 USA. [Garcia, J. Fernando] Univ Estadual Paulista, UNESP, Aracatuba, SP, Brazil. [da Silva, Marcos V. B.] Empresa Brasileira Pesquisa Agr, BR-36038330 Juiz De Fora, MG, Brazil. RP O'Brien, AMP (reprint author), Univ Nat Resources & Life Sci BOKU, Dept Sustainable Agr Syst, Gregor Mendel Str 33, A-1180 Vienna, Austria. EM anita_op@students.boku.ac.at; gabor.meszaros@boku.ac.at; ytutsunomiya@gmail.com; tad.sonstegard@ars.usda.gov; jfgarcia@fmva.unesp.br; curt.vantassell@ars.usda.gov; rcar@fcav.unesp.br; marcos.vb.silva@embrapa.br; johann.soelkner@boku.ac.at RI Carvalheiro, Roberto/I-2239-2013; Utsunomiya, Yuri Tani/K-5214-2013 OI Carvalheiro, Roberto/0000-0002-4506-0555; Utsunomiya, Yuri Tani/0000-0002-6526-8337 FU USDA-ARS [1265-31000-104-00D]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) [560922/2010-8, 483590/2010-0]; Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/16643-2, 2010/52030-2] FX We gratefully acknowledge the Bundesministerium fur Land- und Forstwirtschaft, Umwelt und Wasserwirtschaft (Austria) for data on 29 BRS animals; ZuchtData EDV-Dienstleistungen GmbH (Austria) for data on 96 FLK individuals; Embrapa Gado de Leite (Brazil) for providing data on 28 GIR animals; Guilherme Penteado Coelho Filho, Daniel Biluca, Adriana Santana do Carmo and DeltaGen (Brazil) for technical assistance in sample acquisition and genotyping of 80 NEL animals; and Hapmap Bovine genome project for the rest of the data used for this analysis. This work was supported in part by Projects 1265-31000-104-00D from USDA-ARS, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) - process 560922/2010-8 and 483590/2010-0; and Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) - process 2011/16643-2 and 2010/52030-2. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the authors or their respective institutions. The USDA is an equal opportunity provider and employer. NR 61 TC 10 Z9 11 U1 0 U2 9 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD AUG PY 2014 VL 166 SI SI BP 121 EP 132 DI 10.1016/j.livsci.2014.05.007 PG 12 WC Agriculture, Dairy & Animal Science SC Agriculture GA AO0JD UT WOS:000340994000016 ER PT J AU Fortes, MRS Suhaimi, AHMS Porto-Neto, LR McWilliam, SM Flatscher-Bader, T Moore, SS D'Occhio, MJ Meira, CT Thomas, MG Snelling, WM Reverter, A Lehnert, SA AF Fortes, Marina R. S. Suhaimi, Ainu H. M. S. Porto-Neto, Laercio R. McWilliam, Sean M. Flatscher-Bader, Trudi Moore, Stephen S. D'Occhio, Michael J. Meira, Camila T. Thomas, Milton G. Snelling, Warren M. Reverter, Antonio Lehnert, Sigrid A. TI Post-partum anoestrus in tropical beef cattle: A systems approach combining gene expression and genome-wide association results SO LIVESTOCK SCIENCE LA English DT Article DE Zebu; Hypothalamus; Genomics; Microarray; Anoestrus; Fertility ID RAT HYPOTHALAMUS; SYNAPTOTAGMIN I; COWS; PERFORMANCE; PITUITARY; SELECTION; ESTROGEN; HORMONE; REPRODUCTION; ENVIRONMENT AB The length of the post-partum anoestrous interval affects reproductive efficiency in many tropical beef cattle herds. In this study, results from genome-wide association studies (Experiment 1: GWAS) and gene expression (Experiment 2: microarray) were combined in a systems approach to reveal genetic markers, genes and pathways underlying the physiology of post-partum anoestrus in tropically adapted cattle. The microarray study measured the expression of 13,964 genes in the hypothalamus of Brahman cows. A total of 366 genes were differentially expressed (DE) in the post-partum period, when acyclic cows were compared to cows that had resumed ovarian cycles. Associated markers (P < 0.05) from a high density GWAS pointed to 2829 genes that were associated with post-partum anoestrous interval (PPAI) in two populations of beef cattle: Brahman and Tropical composite. Together the experiments provided evidence for 63 genes that are likely to influence the resumption of ovulation post-partum in tropically adapted beef cattle. Functional annotation analysis revealed that some of the 63 genes have known roles in hormonal activity, energy balance and neuronal synapse plasticity. Polymorphisms within candidate genes identified by this systems approach could have biological significance in post-partum anoestrus and help select Zebu (Bos indicus) influenced cattle with genetic potential for shorter post-partum anoestrus. Crown Copyright (C) 2014 Published by Elsevier B.V. All rights reserved. C1 [Fortes, Marina R. S.; Moore, Stephen S.; D'Occhio, Michael J.] Univ Queensland, Queensland Alliance Agr & Food Innovat, Ctr Anim Sci, Brisbane, Qld 4062, Australia. [Suhaimi, Ainu H. M. S.] MARDI, Strateg Livestock Res Ctr, Kuala Lumpur, Malaysia. [Porto-Neto, Laercio R.; McWilliam, Sean M.; Reverter, Antonio; Lehnert, Sigrid A.] Commonwealth Sci & Ind Res Org Agr Flagship, St Lucia, Qld 4067, Australia. [Flatscher-Bader, Trudi] Univ Queensland, Dept Biochem & Mol Biol, Brisbane, Qld 4072, Australia. [D'Occhio, Michael J.] Univ Sydney, Fac Agr & Environm, Sydney, NSW 2006, Australia. [Meira, Camila T.] Univ Estadual Paulista, Fac Ciencias Agr Vet, BR-14884900 Jaboticabal, SP, Brazil. [Thomas, Milton G.] Colorado State Univ, Dept Anim Sci, Ft Collins, CO 80523 USA. [Snelling, Warren M.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Lehnert, SA (reprint author), Commonwealth Sci & Ind Res Org Agr Flagship, St Lucia, Qld 4067, Australia. EM Sigrid.lehnert@csiro.au RI Porto-Neto, Laercio/D-2594-2012; Fortes, Marina/G-2302-2012; Lehnert, Sigrid/A-3676-2013 OI Porto-Neto, Laercio/0000-0002-3536-8265; Fortes, Marina/0000-0002-7254-1960; NR 47 TC 3 Z9 3 U1 0 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1871-1413 EI 1878-0490 J9 LIVEST SCI JI Livest. Sci. PD AUG PY 2014 VL 166 SI SI BP 158 EP 166 DI 10.1016/j.livsci.2014.06.017 PG 9 WC Agriculture, Dairy & Animal Science SC Agriculture GA AO0JD UT WOS:000340994000019 ER PT J AU Rossman, A Udayanga, D Castlebury, LA Hyde, KD AF Rossman, Amy Udayanga, Dhanushka Castlebury, Lisa A. Hyde, Kevin D. TI (2304) Proposal to conserve the name Diaporthe eres against twenty-one competing names (Ascomyeota: Diaporthales: Diaporthaceae) SO TAXON LA English DT Editorial Material C1 [Rossman, Amy; Udayanga, Dhanushka; Castlebury, Lisa A.] ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. [Udayanga, Dhanushka; Hyde, Kevin D.] Mae Fah Luang Univ, Sch Sci, Inst Excellence Fungal Res, Chiang Rai 57100, Thailand. RP Rossman, A (reprint author), ARS, Systemat Mycol & Microbiol Lab, USDA, Beltsville, MD 20705 USA. EM Amy.Rossman@ARS.USDA.GOV NR 0 TC 3 Z9 3 U1 1 U2 1 PU INT ASSOC PLANT TAXONOMY-IAPT PI BRATISLAVA PA C/O INST BOTANY, SLOVAK ACAD SCIENCES DUBRAVSKA CESTA 9, SK-845 23 BRATISLAVA, SLOVAKIA SN 0040-0262 EI 1996-8175 J9 TAXON JI Taxon PD AUG PY 2014 VL 63 IS 4 BP 934 EP 935 PG 2 WC Plant Sciences; Evolutionary Biology SC Plant Sciences; Evolutionary Biology GA AO0EU UT WOS:000340982700024 ER PT J AU Sweazea, KL McMurtry, JP Elsey, RM Redig, P Braun, EJ AF Sweazea, Karen L. McMurtry, John P. Elsey, Ruth M. Redig, Patrick Braun, Eldon J. TI Comparison of metabolic substrates in alligators and several birds of prey SO ZOOLOGY LA English DT Article DE Carnivore; Metabolic rate; Glucose; Insulin; Fatty acid ID RED-TAILED HAWKS; AMINO-ACID-SEQUENCE; ENDOCRINE PANCREAS; CHICKEN PANCREAS; PLASMA-INSULIN; CARBOHYDRATE-METABOLISM; COMPARATIVE HEMATOLOGY; BLOOD BIOCHEMISTRY; CARNIVOROUS BIRD; GLUCOSE-LEVELS AB On average, avian blood glucose concentrations are 1.5-2 times those of mammals of similar mass and high concentrations of insulin are required to lower blood glucose. Whereas considerable data exist for granivorous species, few data are available for plasma metabolic substrate and glucoregulatory hormone concentrations for carnivorous birds and alligators. Birds and mammals with carnivorous diets have higher metabolic rates than animals consuming diets with less protein whereas alligators have low metabolic rates. Therefore, the present study was designed to compare substrate and glucoregulatory hormone concentrations in several birds of prey and a phylogenetically close relative of birds, the alligator. The hypothesis was that the combination of carnivorous diets and high metabolic rates favored the evolution of greater protein and fatty acid utilization leading to insulin resistance and high plasma glucose concentrations in carnivorous birds. In contrast, it was hypothesized that alligators would have low substrate utilization attributable to a low metabolic rate. Fasting plasma substrate and glucoregulatory hormone concentrations were compared for bald eagles (Haliaeetus leucocephalus), great horned owls (Bubo virginianus), red-tailed hawks (Buteo jamaicensis), and American alligators (Alligator mississippiensis). Avian species had high circulating beta-hydroxybutyrate (10-21 mg/dl) compared to alligators (2.81 +/- 0.16 mg/dl). In mammals high concentrations of this byproduct of fatty acid utilization are correlated with insulin resistance. Fasting glucose and insulin concentrations were positively correlated in eagles whereas no relationship was found between these variables for owls, hawks or alligators. Additionally, p-hydroxybutyrate concentrations were low in alligators. Similar to carnivorous mammals, ingestion of a high protein diet may have favored the utilization of fatty acids and protein for energy thereby promoting the development of insulin resistance and gluconeogenesis-induced high plasma glucose concentrations during periods of fasting in birds of prey. (C) 2014 Elsevier GmbH. All rights reserved. C1 [Sweazea, Karen L.] Arizona State Univ, Sch Nutr & Hlth Promot, Phoenix, AZ 85004 USA. [Sweazea, Karen L.] Arizona State Univ, Sch Life Sci, Tempe, AZ 85287 USA. [McMurtry, John P.] USDA, Growth Biol Lab, Beltsville, MD 20705 USA. [Elsey, Ruth M.] Louisiana Dept Wildlife & Fisheries, Off Wildlife, Grand Chenier, LA 70643 USA. [Redig, Patrick] Univ Minnesota, Coll Vet Med, Raptor Ctr, St Paul, MN 55108 USA. [Braun, Eldon J.] Univ Arizona, Dept Physiol, Tucson, AZ 85724 USA. RP Sweazea, KL (reprint author), Arizona State Univ, Sch Life Sci, 401 East Tyler Mall, Tempe, AZ 85287 USA. EM Karen.Sweazea@asu.edu NR 76 TC 1 Z9 2 U1 5 U2 29 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0944-2006 J9 ZOOLOGY JI Zoology PD AUG PY 2014 VL 117 IS 4 BP 253 EP 260 DI 10.1016/j.zoo1.2014.04.002 PG 8 WC Zoology SC Zoology GA AO0CV UT WOS:000340977600005 PM 25043840 ER PT J AU Lee, B Zhang, RH Du, WX Grauke, LJ McHugh, TH Zhang, YZ AF Lee, BoRam Zhang, Renhao Du, Wen-Xian Grauke, Larry J. McHugh, Tara H. Zhang, Yu-Zhu TI Expression, purification and crystallization of pecan (Carya illinoinensis) vicilin SO ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS LA English DT Article ID MAJOR PEANUT ALLERGEN; ARA H 1; PROTEIN; CLONING; SUPERFAMILY; CRYSTALS AB Tree nuts are responsible for many cases of severe food allergies. The 7S seed storage protein vicilin has been identified as a food allergen in many kinds of tree nuts. The vicilin protein consists of an N-terminal low-complexity region with antimicrobial activity and a C-terminal domain that forms a trimeric structure that belongs to the cupin superfamily. In this study, vicilin from pecan (Carya illinoinensis) was isolated and was expressed in bacteria for the first time. The cupin structural core of the protein, residues 369-792, was purified by metal-affinity and gel-filtration chromatography to high purity. Vicilin crystals were obtained and the best crystal diffracted to 2.65 angstrom resolution in space group P2(1)2(1)2(1). C1 [Lee, BoRam; Zhang, Renhao; Du, Wen-Xian; McHugh, Tara H.; Zhang, Yu-Zhu] ARS, Hlth Proc Foods Res Unit, USDA, PWA,Western Reg Res Ctr, Albany, CA 94710 USA. [Grauke, Larry J.] ARS, USDA, SPA, Southern Plains Agr Res Ctr, College Stn, TX 77845 USA. RP Zhang, YZ (reprint author), ARS, Hlth Proc Foods Res Unit, USDA, PWA,Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM yuzhu.zhang@ars.usda.gov OI Zhang, Yuzhu/0000-0001-7882-5692 FU National Institutes of Health, National Institute of General Medical Sciences; Office of Science, Office of Basic Energy Sciences of the US Department of Energy [DE-AC02-05CH11231] FX Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. The Berkeley Center for Structural Biology is supported in part by the National Institutes of Health, National Institute of General Medical Sciences. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences of the US Department of Energy under Contract No. DE-AC02-05CH11231. NR 20 TC 2 Z9 2 U1 1 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1744-3091 J9 ACTA CRYSTALLOGR F JI Acta Crystallogr. F-Struct. Biol. Commun. PD AUG PY 2014 VL 70 BP 1049 EP 1052 DI 10.1107/S2053230X14012369 PN 8 PG 4 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography SC Biochemistry & Molecular Biology; Biophysics; Crystallography GA AN0BL UT WOS:000340246500009 PM 25084379 ER PT J AU Paranandi, A Asztalos, BF Mangili, A Kuvin, J Gerrior, J Sheehan, H Skinner, SC Tang, AM Wanke, CA AF Paranandi, Anu Asztalos, Bela F. Mangili, Alexandra Kuvin, Jefferey Gerrior, Jul Sheehan, Heidi Skinner, Sally C. Tang, Alice M. Wanke, Christine A. TI Short Communication: Effects of Omega-3 Fatty Acids on Triglycerides and High-Density Lipoprotein Subprofiles in HIV-Infected Persons with Hypertriglyceridemia SO AIDS RESEARCH AND HUMAN RETROVIRUSES LA English DT Article ID POLYUNSATURATED FATTY-ACIDS; CORONARY HEART-DISEASE; ANTIRETROVIRAL THERAPY; FISH-OIL; CARDIOVASCULAR EVENTS; INTERVENTION TRIAL; HDL SUBPOPULATIONS; ATHEROSCLEROSIS; SUPPLEMENTATION; ASSOCIATION AB Hypertriglyceridemia and low high-density lipoprotein (HDL)-cholesterol (HDL-C) may contribute to a presumed accelerated risk for cardiovascular disease in HIV-infected individuals. We evaluated the effect of omega-3 fatty acid treatment on triglycerides, low-density lipoprotein (LDL)-C, HDL-C, and HDL subpopulations. Forty-one HIV-seropositive subjects with hypertriglyceridemia (150mg/dl) on active antiretroviral therapy were enrolled in this placebo-controlled, double-blind, randomized, crossover trial comparing the effects of omega-3 fatty acid treatment (1.9 g EPA and 1.5 g DHA) on triglycerides, LDL-C, HDL-C, and HDL subpopulations. An independent sample t-test was used to assess the study start to posttreatment change for all components. After omega-3 fatty acid treatment, triglyceride levels decreased 63.2 +/- 86.9 mg/dl (p < 0.001). No significant changes in total cholesterol, LDL-C, or HDL-C were found. Within HDL subpopulations, significant changes were seen in the most ather-oprotective HDL particles, alpha-1, which increased by 2.5 +/- 5.6mg/dl (p < 0.05), and pre alpha-1, which increased by 0.6 +/- 1.0mg/dl (p < 0.001). Pre alpha-3, a presumably atherogenic HDL particle, decreased by 0.5 +/- 0.9mg/dl (p < 0.01). Omega-3 fatty acid treatment significantly lowered triglyceride levels in HIV-positive patients with moderate hypertriglyceridemia. While no study-wide improvements in LDL-C or HDL-C were detected, the HDL subpopulation profile changed in a beneficial way suggesting more cardioprotection after treatment. C1 [Paranandi, Anu; Mangili, Alexandra; Wanke, Christine A.] Tufts Med Ctr, Div Geog Med & Infect Dis, Boston, MA USA. [Asztalos, Bela F.] Tufts Univ, Human Nutr Res Ctr Aging, Lipid Metab Lab, Boston, MA 02111 USA. [Mangili, Alexandra; Gerrior, Jul; Sheehan, Heidi; Skinner, Sally C.; Tang, Alice M.; Wanke, Christine A.] Tufts Univ, Sch Med, Dept Publ Hlth & Community Med, Nutr Infect Unit, Boston, MA 02111 USA. [Kuvin, Jefferey] Tufts Med Ctr, Div Cardiol, Boston, MA USA. RP Paranandi, A (reprint author), 150 Harrison Ave,Jaharis Room 268, Boston, MA 02111 USA. EM jogan1122@hotmail.com; cwanke@tuftsmedicalcenter.org FU Lifespan/Tufts/Brown Center for AIDS Research, NIH/NHLBI [5R01 HL065947-10]; NIH [5T32A1007438-19]; CFAR [1 P30 AI42853-12] FX This study was supported by the Lifespan/Tufts/Brown Center for AIDS Research, NIH/NHLBI 5R01 HL065947-10, NIH 5T32A1007438-19, and CFAR grant 1 P30 AI42853-12. Data collection, statistical analysis plan, and analyses were designed and performed by the Tufts University School of Medicine. The article was prepared by Dr. Wanke and the study team members. NR 25 TC 5 Z9 5 U1 0 U2 5 PU MARY ANN LIEBERT, INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 0889-2229 EI 1931-8405 J9 AIDS RES HUM RETROV JI Aids Res. Hum. Retrovir. PD AUG PY 2014 VL 30 IS 8 BP 800 EP 805 DI 10.1089/aid.2014.0005 PG 6 WC Immunology; Infectious Diseases; Virology SC Immunology; Infectious Diseases; Virology GA AN4DX UT WOS:000340538900012 PM 24988179 ER PT J AU Arbellay, E Stoffel, M Sutherland, EK Smith, KT Falk, DA AF Arbellay, Estelle Stoffel, Markus Sutherland, Elaine K. Smith, Kevin T. Falk, Donald A. TI Changes in tracheid and ray traits in fire scars of North American conifers and their ecophysiological implications SO ANNALS OF BOTANY LA English DT Article DE Ecophysiology; conifer; fire scar; Larix occidentalis; western larch; Pinus ponderosa; ponderosa pine; Pseudotsuga menziesii; Douglas fir; ray; tracheid; wood anatomy ID TRAUMATIC RESIN DUCTS; PONDEROSA PINE FOREST; LARIX-DECIDUA; TREE-RINGS; HYDRAULIC ARCHITECTURE; ANATOMICAL CHANGES; EUROPEAN CONIFERS; TANGENTIAL ROWS; BARK PROPERTIES; VASCULAR RAYS AB Background and Aims Fire scars have been widely used as proxies for the reconstruction of fire history; however, little is known about the impact of fire injury on wood anatomy. This study investigates changes in tracheid and ray traits in fire scars of Douglas fir (Pseudotsuga menziesii), western larch (Larix occidentalis) and ponderosa pine (Pinus ponderosa), and discusses their ecophysiological implications for tree recovery from fire. Methods Transverse and tangential microsections were prepared for light microscopy and image analysis. Measurements of tracheids and rays were made in the three spatial dimensions: axially (at different section heights), radially (in different rings) and tangentially (with increasing distance from the wound margin). Key Results Changes were strongest in the first year after fire injury, with a decrease in tracheid size (by 25-30%) and an increase in tracheid density (by 21-53%) for the three species. In addition, an increase in ray size (by 5-27 %) and an increase in ray density (by 19-36%) were found in P. menziesii and L. occidentalis. Changes were comparable along the fire-injured stem and were often most marked close to the fire scar. Conclusions The differentiation after fire injury of narrower and more numerous tracheids expresses a trade-off between hydraulic safety and hydraulic efficiency, while that of larger and more numerous rays serves compartmentalization and wound closure, mechanical strength and defence responses. Pinus ponderosa does not generally produce more ray tissue after fire injury and thus appears to be more adapted to fire. C1 [Arbellay, Estelle; Stoffel, Markus] Univ Bern, Inst Geol Sci, Dendrolab Ch, Bern, Switzerland. [Stoffel, Markus] Univ Geneva, Inst Environm Sci, Geneva, Switzerland. [Sutherland, Elaine K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT USA. [Smith, Kevin T.] US Forest Serv, USDA, No Res Stn, Durham, NH USA. [Falk, Donald A.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ USA. [Falk, Donald A.] Univ Arizona, Tree Ring Res Lab, Tucson, AZ 85721 USA. RP Arbellay, E (reprint author), Univ British Columbia, Tree Ring Lab, Vancouver, BC V5Z 1M9, Canada. EM arbellay@mail.ubc.ca RI Stoffel, Markus/A-1793-2017 OI Stoffel, Markus/0000-0003-0816-1303 FU US Forest Service Research and Development; National Fire Plan Research funds FX We thank the Lolo National Forest, Missoula Ranger District for their co-operation during the planning and sampling phase of this study. This work was supported in part by US Forest Service Research and Development and National Fire Plan Research funds. We are grateful for the assistance of Josh Farella, David K. Wright and especially Ian R. Hyp (all US Forest Service Rocky Mountain Research Station) for sampling and preparing the cross-sections. NR 86 TC 10 Z9 10 U1 1 U2 36 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0305-7364 EI 1095-8290 J9 ANN BOT-LONDON JI Ann. Bot. PD AUG PY 2014 VL 114 IS 2 BP 223 EP 232 DI 10.1093/aob/mcu112 PG 10 WC Plant Sciences SC Plant Sciences GA AN2RY UT WOS:000340434200003 PM 24941999 ER PT J AU Poessel, SA Gese, EM Young, JK AF Poessel, Sharon A. Gese, Eric M. Young, Julie K. TI Influence of habitat structure and food on patch choice of captive coyotes SO APPLIED ANIMAL BEHAVIOUR SCIENCE LA English DT Article DE Boldness; Canid; Canis latrans; Carnivore; Personality; Urban ID CHICAGO METROPOLITAN-AREA; CANIS-LATRANS; HOME-RANGE; BEHAVIORAL SYNDROMES; SOUTHERN CALIFORNIA; URBAN-ENVIRONMENT; SELECTION; ECOLOGY; URBANIZATION; BIODIVERSITY AB Increasing urban development can have significant effects on wildlife species, including carnivores. Some carnivore species have been able to adapt to and even thrive in urban environments, including coyotes (Canis latrans). The presence of carnivores in urban areas can sometimes lead to conflicts with humans and their pets. Although coyotes may frequently use urban areas, they also inhabit natural areas surrounding urban development. Understanding the various factors affecting patch choice of urban coyotes may assist wildlife officials in managing human-coyote conflicts. Both sex and behavioral profile can influence patch choice; bold individuals are more likely to be exploratory than shy animals, which can result in increased conflicts in urban environments. Using a captive population of coyotes, we tested whether coyotes preferred urban (anthropogenic) habitat structure, natural structure, or a mixture of structures and whether sex, behavioral profile, biological season, or food manipulation affected coyote patch choice. Coyotes generally preferred the control, homogeneous structure representing their natal habitat (mean percentage of time 23.3% +/- 19.3 SD; P = 0.037). The next most preferred habitat for coyotes, especially females (23.7 +/- 16.6% for 25% urban pen; P = 0.020) and bold coyotes (27.8 +/- 23.2% for 75% urban pen; P = 0.005), was a mixture of anthropogenic and natural structures rather than uniform structure (all natural or all anthropogenic), and this preference was more strongly expressed during the non-breeding season (25.6 +/- 23.2% for 75% urban pen; P = 0.017). Food had no effect on patch choice (P = 0.983); coyotes appeared to be primarily motivated by the structure of the habitat rather than by the amount of food within each habitat. Our results suggested that urban areas containing large amounts of both natural and anthropogenic structures are more likely to be used by coyotes and, thus, could have the potential for human-coyote conflicts. (C) 2014 Elsevier B.V. All rights reserved. C1 [Poessel, Sharon A.; Gese, Eric M.; Young, Julie K.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Gese, Eric M.; Young, Julie K.] USDA, WS, Natl Wildlife Res Ctr, Logan, UT 84322 USA. RP Poessel, SA (reprint author), Utah State Univ, Dept Wildland Resources, 5230 Old Main Hill, Logan, UT 84322 USA. EM sharpoes@gmail.com RI Poessel, Sharon/B-3651-2013; Gese, Eric/B-4578-2011 OI Poessel, Sharon/0000-0002-0283-627X; FU U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Logan Field Station [12-7449-0042]; Ecology Center at Utah State University FX We thank the employees and volunteers at the National Wildlife Research Center Predator Research Facility for their assistance with this study, including S. Brummer, J. Schultz, B. McDonald, E. Stevenson, M. Davis, E. Miller, L. le Caro, A. Legros, C. Boone, L. Ferguson, E. Wright, B. Jones, M. Belz, D. Napoletano, M. Mahe, C. Wallace, B. Orning, L. Blake, and M. Stevenson. We also thank S. Durham for statistical advice. Funding and logistical support were provided by the U.S. Department of Agriculture, Wildlife Services, National Wildlife Research Center, Logan Field Station, Cooperative Research Agreement 12-7449-0042 and the Ecology Center at Utah State University. NR 57 TC 3 Z9 3 U1 8 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0168-1591 EI 1872-9045 J9 APPL ANIM BEHAV SCI JI Appl. Anim. Behav. Sci. PD AUG PY 2014 VL 157 BP 127 EP 136 DI 10.1016/j.applanim.2014.05.001 PG 10 WC Agriculture, Dairy & Animal Science; Behavioral Sciences; Veterinary Sciences SC Agriculture; Behavioral Sciences; Veterinary Sciences GA AN1FS UT WOS:000340328700014 ER PT J AU Green, BW Schrader, KK Perschbacher, PW AF Green, Bartholomew W. Schrader, Kevin K. Perschbacher, Peter W. TI Effect of stocking biomass on solids, phytoplankton communities, common off-flavors, and production parameters in a channel catfish biofloc technology production system SO AQUACULTURE RESEARCH LA English DT Article DE Biofloc technology; stocker channel catfish; off-flavor; phytoplankton; geosmin; 2-methylisoborneol ID ICTALURUS-PUNCTATUS PONDS; AQUACULTURE SYSTEMS; MINIMAL-EXCHANGE; WATER-QUALITY; CULTURE; GEOSMIN; GROWTH; PHASE; 2-METHYLISOBORNEOL; SIZE AB The effect of initial channel catfish (Ictalurus punctatus, Rafinesque, 1818) fingerling biomass (1.4, 1.8, or 2.3 kg m(-3)) on phytoplankton communities, common off-flavours and stocker catfish production parameters was evaluated in biofloc technology production tanks. Stocker catfish size (145.5-172.6 g fish(-1)) at harvest did not differ among treatments, but net yield increased linearly as initial biomass increased (R-2 = 0.633). Mean total feed consumption increased linearly with initial catfish biomass (R-2 = 0.656) and ranged from 10.7 to 15.8 kg m(-3). Total suspended solids (TSS) in all treatments increased linearly with total feed addition, and high TSS appeared to impact negatively daily feed consumption. Initial phytoplankton populations were dominated by small colonial green algae and diatoms, and later transitioned to populations dominated by a small, filamentous cyanobacteria and diatoms. Low, variable concentrations of 2-methylisoborneol and geosmin were present in biofloc tank water during most of the study and two tanks yielded catfish with 2-methylisoborneol or geosmin concentrations that might be classified as off-flavour. One isolate of actinomycete was isolated sporadically from some biofloc tanks, but its abundance was not correlated with 2-methylisoborneol concentration in tank waters. The microbial sources of 2-methylisoborneol and geosmin in biofloc tanks remain unidentified. C1 [Green, Bartholomew W.] ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Schrader, Kevin K.] ARS, USDA, Nat Prod Utilizat Res Unit, Thad Cochran Natl Ctr Nat Prod Res, University, MS USA. [Perschbacher, Peter W.] Univ Arkansas Pine Bluff, Aquaculture Fisheries Ctr, Pine Bluff, AR USA. RP Green, BW (reprint author), ARS, USDA, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. EM Bart.Green@ars.usda.gov FU USDA/ARS [6225-31630-006-00D] FX We thank Dr Kathy Yeater for her assistance with and informative discussion of PROC GLIMMIX. The technical assistance of Greg O'Neal, Matt McEntire, Dewayne Harries and Phaedra Page is greatly appreciated. This study was funded by the USDA/ARS under project number 6225-31630-006-00D. No sources of funding external to any author institution were used for this study. None of the authors have any conflict of interest. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer. NR 38 TC 1 Z9 2 U1 4 U2 38 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1355-557X EI 1365-2109 J9 AQUAC RES JI Aquac. Res. PD AUG PY 2014 VL 45 IS 9 BP 1442 EP 1458 DI 10.1111/are.12096 PG 17 WC Fisheries SC Fisheries GA AN1PK UT WOS:000340355600003 ER PT J AU Nichols, NN Hector, RE Saha, BC Frazer, SE Kennedy, GJ AF Nichols, Nancy N. Hector, Ronald E. Saha, Badal C. Frazer, Sarah E. Kennedy, Gregory J. TI Biological abatement of inhibitors in rice hull hydrolyzate and fermentation to ethanol using conventional and engineered microbes SO BIOMASS & BIOENERGY LA English DT Article DE Rice hulls; Fermentation; Inhibitors; Bioabatement; Hemicellulose ID DILUTE-ACID PRETREATMENT; ESCHERICHIA-COLI STRAINS; SACCHAROMYCES-CEREVISIAE; ACETIC-ACID; XYLOSE FERMENTATION; DETOXIFICATION; HYDROLYSATE; REDUCTASE; BIODETOXIFICATION; SACCHARIFICATION AB Microbial inhibitors arise from lignin, hemicellulose, and degraded sugar during pretreatment of lignocellulosic biomass. The fungus Coniochaeta ligniaria NRRL30616 has native ability to metabolize a number of these compounds, including furan and aromatic aldehydes known to act as inhibitors toward relevant fermenting microbes. In this study, C. ligniaria was used to metabolize and remove inhibitory compounds from pretreated rice hulls, which comprise a readily available agricultural residue rich in glucose (0.32-0.33 g glucan/g hulls) and xylose (0.15-0.19 g xylan/g hulls). Samples were dilute-acid pretreated and subjected to bioabatement of inhibitors by C. ligniaria. The bioabated rice hull hemicellulose hydrolyzates were then utilized for ethanol fermentations. In bioabated liquors, glucose was converted to 0.58% (w/v) ethanol by Saccharomyces cerevisiae D5a at 100% of theoretical yield, while fermentations of unabated hydrolyzates either failed to exit lag phase or had reduced ethanol yield (80% of theoretical). In fermentations using ethanologens engineered for conversion of pentoses, bioabatement of hydrolyzates similarly improved fermentations. Fermentation of xylose and arabinose by ethanologenic Escherichia coli FBR5 yielded 2.25% and 0.05% (w/v) ethanol from bioabated and unabated samples, respectively. Fermentations using S. cerevisiae YRH400 had decreased fermentation lag times in bioabated hydrolyzates. However, xylose metabolism in S. cerevisiae YRH400 was strongly affected by pH and acetate concentration. Published by Elsevier Ltd. C1 [Nichols, Nancy N.; Hector, Ronald E.; Saha, Badal C.; Frazer, Sarah E.; Kennedy, Gregory J.] USDA ARS, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. RP Nichols, NN (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, 1815 N Univ St, Peoria, IL 61604 USA. EM nancy.nichols@ars.usda.gov NR 39 TC 6 Z9 6 U1 2 U2 16 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0961-9534 EI 1873-2909 J9 BIOMASS BIOENERG JI Biomass Bioenerg. PD AUG PY 2014 VL 67 BP 79 EP 88 DI 10.1016/j.biombioe.2014.04.026 PG 10 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AN1JX UT WOS:000340339700009 ER PT J AU Lichtenstein, AH AF Lichtenstein, Alice H. TI Dietary Trans Fatty Acids and Cardiovascular Disease Risk: Past and Present SO CURRENT ATHEROSCLEROSIS REPORTS LA English DT Article DE Trans fatty acids; Nutrition; Diet; Public policy; Cardiovascular disease; Polyunsaturated fatty acids; Monounsaturated fatty acids ID LOW-DENSITY-LIPOPROTEIN; CORONARY-HEART-DISEASE; MODERATELY HYPERCHOLESTEROLEMIC WOMEN; HYDROGENATED FAT; CHOLESTEROL LEVELS; CONTROLLED-TRIALS; DIFFERENT FORMS; CONSUMPTION; ADULTS; HEALTH AB Dietary trans double bond containing fatty acids have been associated with increased risk of cardiovascular disease. There are two main sources of dietary trans fatty acids: meat and dairy fats, and partially hydrogenated fats. Because of a number of factors, including changes in federal labeling requirements for packaged foods, and local bans and grassroots pressure on the use of partially hydrogenated fat, trans fat intake has declined in recent years. Similar to saturated fatty acids, trans fatty acids increase plasma low density lipoprotein (LDL)-cholesterol concentrations. In contrast to saturated fatty acids, trans fatty acids do not increase high density lipoprotein (HDL)-cholesterol concentrations. These differences have been attributed to lipoprotein catabolic rate rather than production rate. When reported, effects of partially hydrogenated fat on glucose homeostasis, C-reactive protein, blood pressure, and LDL oxidation are modest. Although at this time some issues remain unresolved regarding trans fatty acids and CVD risk factors other than plasma lipoprotein concentrations, they should not affect the final dietary recommendation to limit intake. C1 Tufts Univ, JM USDA Human Nutr Res Ctr, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, JM USDA Human Nutr Res Ctr, 711 Washington St, Boston, MA 02111 USA. EM alice.lichtenstein@tufts.edu NR 54 TC 13 Z9 13 U1 4 U2 63 PU CURRENT MEDICINE GROUP PI PHILADELPHIA PA 400 MARKET STREET, STE 700, PHILADELPHIA, PA 19106 USA SN 1523-3804 EI 1534-6242 J9 CURR ATHEROSCLER REP JI Curr. Atheroscleros. Rep. PD AUG PY 2014 VL 16 IS 8 AR 433 DI 10.1007/s11883-014-0433-1 PG 7 WC Peripheral Vascular Disease SC Cardiovascular System & Cardiology GA AN3XZ UT WOS:000340523400006 PM 24907053 ER PT J AU Hamerlynck, EP Scott, RL Cavanaugh, ML Barron-Gafford, G AF Hamerlynck, Erik P. Scott, Russell L. Cavanaugh, Michelle L. Barron-Gafford, Greg TI Water use efficiency of annual-dominated and bunchgrass-dominated savanna intercanopy space SO ECOHYDROLOGY LA English DT Article DE evapotranspiration; net ecosystem carbon exchange; phenology; photosynthesis; respiration; transpiration ID NORTHERN TEMPERATE GRASSLAND; SOIL CO2 FLUX; SAGEBRUSH COMMUNITIES; VEGETATION PATTERNS; CLIMATE-CHANGE; GREAT-BASIN; ECOSYSTEM; CARBON; EXCHANGE; GRASSES AB In semi-arid savannas, dominance of intercanopy space by annual or perennial grasses may alter partitioning of ecosystem water and carbon fluxes and affect ecosystem water use efficiency (WUEe), the ratio of net ecosystem carbon dioxide exchange (NEE) to evapotranspiration (ET). To establish if these contrasting growth habits changed controls to WUEe, we tracked volumetric soil moisture (theta(25cm)), ET and transpiration (T), NEE and its constituent ecosystem respiration (R-eco) and gross ecosystem photosynthesis (GEP) fluxes, and community water use efficiency (WUEc = GEP : T) in annual-dominated and bunchgrass-dominated plots in a southern Arizona, United States, savanna. Annual and bunchgrass plots had similar theta(25cm), ET, and T, suggesting the similarity in ET was due to higher soil evaporation in annual plots. Seasonal NEE was delayed and lower in annual plots compared with that in bunchgrass plots, owing to higher Reco in annual plots. Transpiration, GEP, and R-eco in both vegetation types increased following late-season rain, indicating similar late-season phenological constraint. WUEe was lower in annual plots, but with similar WUEc between plot types. These results suggest that differences in annual plant biomass allocation and plot-level leaf area distribution increased proportional soil evaporation and aboveground R-eco contributions, reducing plot-level WUEe, not lowering plant WUE typical of arid-land annuals. Lower plot-level WUEe suggests that any increase in annual plant dominance would increase interannual variation of productivity in savanna intercanopy spaces, which could enhance the negative effects of predicted higher temperatures, greater aridity, and larger and more widely spaced storms on arid-land watershed processes. Published 2013. This article is a U. S. Government work and is in the public domain in the USA. C1 [Hamerlynck, Erik P.; Scott, Russell L.; Cavanaugh, Michelle L.] USDA ARS, Southwest Watershed Res Ctr, Tucson, AZ 85719 USA. [Barron-Gafford, Greg] Univ Arizona, Tucson, AZ 85721 USA. RP Hamerlynck, EP (reprint author), USDA ARS, Southwest Watershed Res Ctr, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM erik.hamerlynck@ars.usda.gov NR 43 TC 3 Z9 4 U1 4 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1936-0584 EI 1936-0592 J9 ECOHYDROLOGY JI Ecohydrology PD AUG PY 2014 VL 7 IS 4 BP 1208 EP 1215 DI 10.1002/eco.1452 PG 8 WC Ecology; Environmental Sciences; Water Resources SC Environmental Sciences & Ecology; Water Resources GA AN4EY UT WOS:000340541600013 ER PT J AU Zeng, LM Zhang, J Han, YC Yang, L Wu, MD Jiang, DH Chen, WD Li, GQ AF Zeng, Li-Mei Zhang, Jing Han, Yong-Chao Yang, Long Wu, Ming-de Jiang, Dao-Hong Chen, Weidong Li, Guo-Qing TI Degradation of oxalic acid by the mycoparasite Coniothyrium minitans plays an important role in interacting with Sclerotinia sclerotiorum SO ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID OXALATE DECARBOXYLASE GENE; NUTRITIONAL FACTORS; TRANSGENIC TOBACCO; COLLYBIA-VELUTIPES; MOLECULAR-CLONING; AMBIENT PH; HOST-PLANT; EXPRESSION; OVEREXPRESSION; RESISTANCE AB Coniothyrium minitans (Cm) is a mycoparasite of the phytopathogenic fungus Sclerotinia sclerotiorum (Ss). Ss produces a virulence factor oxalic acid (OA) which is toxic to plants and also to Cm, and Cm detoxifies OA by degradation. In this study, two oxalate decarboxylase genes, Cmoxdc1 and Cmoxdc2, were cloned from Cm strain Chy-1. OA and low pH induced expression of Cmoxdc1, but not Cmoxdc2. Cmoxdc1 was partially responsible for OA degradation, whereas Cmoxdc2 had no effect on OA degradation. Disruption of Cmoxdc1 in Cm reduced its ability to infect Ss in dual cultures where OA accumulated. Compared with Chy-1, the Cmoxdc1-disrupted mutants had reduced expression levels of two mycoparasitism-related genes chitinase (Cmch1) and beta-1,3-glucanase (Cmg1), and had no detectable activity of extracellular proteases in the presence of OA. On the other hand, the cultural filtrates of the Cmoxdc1-disrupted mutants in OA-amended media showed enhanced antifungal activity, possibly because of increased production of antifungal substances under acidic pH condition resulted from reduced Cmoxdc1-mediated OA degradation. This study provides direct genetic evidence of OA degradation regulating mycoparasitism and antibiosis of Cm against Ss, and sheds light on the sophisticated strategies of Cm in interacting with metabolically active mycelia and dormant sclerotia of Ss. C1 [Zeng, Li-Mei; Zhang, Jing; Han, Yong-Chao; Yang, Long; Wu, Ming-de; Jiang, Dao-Hong; Li, Guo-Qing] Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, State Key Lab Agr Microbiol, Wuhan, Peoples R China. [Chen, Weidong] Washington State Univ, USDA, ARS, Pullman, WA 99164 USA. RP Li, GQ (reprint author), Huazhong Agr Univ, Key Lab Plant Pathol Hubei Prov, State Key Lab Agr Microbiol, Wuhan, Peoples R China. EM guoqingli@mail.hzau.edu.cn FU National Natural Science Foundation of China [31000877, 31272084]; National '973' Basic Research Program of China [2012cb114000]; China's Special Fund for Agroscientific Research in the Public Interest [201103016] FX The authors wish to express appreciation to two anonymous reviewers for valuable suggestions in improving the manuscript. This study was financially supported by the National Natural Science Foundation of China (Grant nos. 31000877, 31272084), National '973' Basic Research Program of China (Grant no. 2012cb114000) and China's Special Fund for Agroscientific Research in the Public Interest (Grant no. 201103016). Dr. Y.-C. Han is now working at the Institute of Industrial Crops of Hubei Academy of Agricultural Sciences (Wuhan, China). NR 58 TC 6 Z9 8 U1 3 U2 36 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1462-2912 EI 1462-2920 J9 ENVIRON MICROBIOL JI Environ. Microbiol. PD AUG PY 2014 VL 16 IS 8 BP 2591 EP 2610 DI 10.1111/1462-2920.12409 PG 20 WC Microbiology SC Microbiology GA AN3XR UT WOS:000340522500018 PM 24467446 ER PT J AU Custer, TW Dummer, PM Custer, CM Franson, JC Jones, M AF Custer, Thomas W. Dummer, Paul M. Custer, Christine M. Franson, J. Christian Jones, Michael TI CONTAMINANT EXPOSURE OF BIRDS NESTING IN GREEN BAY, WISCONSIN, USA SO ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY LA English DT Article DE Tree swallow; Double-crested cormorant; Polychlorinated biphenyls (PCBs); Dioxins; Pesticides ID POLYCHLORINATED BIPHENYL CONGENERS; LOWER FOX RIVER; ACCUMULATION; EGGS; SEDIMENT; PATTERNS; 2,3,7,8-TETRACHLORODIBENZOFURAN; DISPOSITION; METABOLISM; PCBS AB In earlier studies, elevated concentrations of polychlorinated biphenyl (PCB) and p, p'-dichlorodiphenyldichloroethylene (DDE) were reported in double-crested cormorant (Phalacrocorax auritus) eggs and tree swallow (Tachycineta bicolor) eggs and nestlings collected from lower Green Bay (WI, USA) in 1994 and 1995 and black-crowned night-heron (Nycticorax nycticorax) eggs collected in 1991. Comparable samples collected in 2010 and 2011 indicated that concentrations of PCBs were 35%, 62%, 70%, and 88% lower than in the early 1990s in tree swallow eggs, tree swallow nestlings, double-crested cormorant eggs, and black-crowned night-heron eggs, respectively; concentrations of DDE were 47%, 43%, 51%, and 80% lower, respectively. These declines are consistent with regional contaminant trends in other species. Concentrations of PCBs were higher in herring gull (Larus argentatus) than in black-crowned night-heron eggs collected from Green Bay in 2010; PCB concentrations in double-crested cormorant and tree swallow eggs were intermediate. The estimated toxicity of the PCB mixture in eggs of the insectivorous tree swallow was the equal to or greater than toxicity in the 3 piscivorous bird species. A multivariate analysis indicated that the composition percentage of lower-numbered PCB congeners was greater in eggs of the insectivorous tree swallow than in eggs of the 3 piscivorous species nesting in Green Bay. Dioxin and furan concentrations and the toxicity of these chemicals were also higher in tree swallows than these other waterbird species nesting in Green Bay. Environ Toxicol Chem 2014; 33: 1832-1839. Published 2014 Wiley Periodicals, Inc. on behalf of SETAC. This article is a US Government work and, as such, is in the public domain in the USA C1 [Custer, Thomas W.; Dummer, Paul M.; Custer, Christine M.] US Geol Survey, La Crosse, WI 54603 USA. [Franson, J. Christian] US Geol Survey, Madison, WI USA. [Jones, Michael] USDA, Waupun, WI USA. RP Custer, TW (reprint author), US Geol Survey, La Crosse, WI 54603 USA. EM tcuster@usgs.gov OI Custer, Thomas/0000-0003-3170-6519; Franson, J/0000-0002-0251-4238 FU Great Lakes Restoration Initiative FX We thank D. Goldberg, A. Haertel, K. Mott, D. Ripp, J. TeSlaa, and M. Weber for field and data management assistance. We also thank the City of Green Bay for permission to erect nest boxes. The present study was funded by the Great Lakes Restoration Initiative. NR 24 TC 6 Z9 6 U1 3 U2 24 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0730-7268 EI 1552-8618 J9 ENVIRON TOXICOL CHEM JI Environ. Toxicol. Chem. PD AUG PY 2014 VL 33 IS 8 BP 1832 EP 1839 DI 10.1002/etc.2609 PG 8 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA AN4DD UT WOS:000340536900022 PM 24733712 ER PT J AU Park, JH Juzwik, J AF Park, J. -H. Juzwik, J. TI Ceratocystis smalleyi colonization of bitternut hickory and host responses in the xylem SO FOREST PATHOLOGY LA English DT Article ID STEM WATER TRANSPORT; PINE WILT DISEASE; PHYTOPHTHORA-RAMORUM; RAFFAELEA-QUERCIVORA; TYLOSIS FORMATION; NORWAY SPRUCE; OAK TREES; INOCULATION; FLOW; LOCALIZATION AB Colonization of Carya cordiformis sapwood by Ceratocystis smalleyi and subsequent host defence responses following artificial inoculation were investigated using anatomical and histological techniques. Hyphae of C. smalleyi were observed in all sapwood xylem features confirming the ability of the pathogen to invade and colonize the xylem tissues of the host species. The fungus was isolated from within and at the margins of discoloured sapwood areas at 2 and 12 months after inoculation. General host defence responses that included vessel occlusion with gels or tyloses, lipid accumulation, and production of phenolic compounds were observed in xylem tissues of inoculated C. cordiformis stems. Pectic substances, lipids, and to a rare extent, phenolic compounds were detected in vascular gels. The lipid-rich barriers observed likely prevent lateral expansion of the fungus in the sapwood. Furthermore, lack of fungus sporulation within vessels may restrict axial spread of the fungus. C. smalleyi appears to be a limited vascular wilt pathogen of bitternut hickory based on these observations and previously reported sap flow reduction correlated with multiple infections in artificially inoculated trees. C1 [Park, J. -H.] Korea Forest Res Inst, Seoul, South Korea. [Juzwik, J.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP Juzwik, J (reprint author), US Forest Serv, No Res Stn, St Paul, MN 55108 USA. EM jjuzwik@fs.fed.us FU USDA Forest Service, Forest Health Evaluation Monitoring Program [NC-EM-07-01]; Department of Plant Pathology, University of Minnesota FX The technical assistance of the following individuals is gratefully acknowledged: Bill Stroobants, Bobbi Zenner and Paul Castillo. Robert Blanchette is acknowledged for providing guidance for the histological study and for review of the manuscript. Kevin T. Smith, Robert Seavey and Alex Wiedenhoeft are acknowledged for providing equipment, supplies and instructions. Field sites and trees used for this research were provided by management foresters of the Chippewa County Forest in Wisconsin. The authors also thank Neil A. Anderson for presubmission review of the manuscript. Suggestions from two anonymous reviewers are also gratefully acknowledged. The research was supported in part by the USDA Forest Service, Forest Health Evaluation Monitoring Program (project no. NC-EM-07-01). Partial funding also was provided by the Department of Plant Pathology, University of Minnesota. NR 51 TC 0 Z9 0 U1 1 U2 15 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 EI 1439-0329 J9 FOREST PATHOL JI Forest Pathol. PD AUG PY 2014 VL 44 IS 4 BP 282 EP 292 DI 10.1111/efp.12098 PG 11 WC Forestry SC Forestry GA AN3LH UT WOS:000340489000004 ER PT J AU Melo, BLB de Souza, JT Santos, RMF Rehner, SA Solis, KH Suarez, C Hebbar, PK Lemos, LSL Gramacho, KP AF Melo, B. L. B. de Souza, J. T. Santos, R. M. F. Rehner, S. A. Solis, K. H. Suarez, C. Hebbar, P. K. Lemos, L. S. L. Gramacho, K. P. TI Development of microsatellites for the cacao frosty pod rot pathogen, Moniliophthora roreri SO FOREST PATHOLOGY LA English DT Article ID THEOBROMA-CACAO; MARKERS; DNA; POPULATIONS; PERNICIOSA; FUNGI AB Moniliophithora roreri, causal agent of cacao frosty pod rot, is considered one of the most devastating pathogens of this crop. In this study, we report the development of and validation of 28 microsatellite loci from an enriched library. From these, ten loci were demonstrated to be polymorphic in an Ecuadorian population composed of 27 isolates. The mean number of alleles per locus was 3.2, and the observed mean and expected heterozygosity were 0.03 and 0.43, respectively. The polymorphic microsatellites described herein may be used to study the genetic diversity as well as to comprehend other aspects of M. roreri population biology. C1 [Melo, B. L. B.; Santos, R. M. F.; Lemos, L. S. L.; Gramacho, K. P.] Cacao Res Ctr, Itabuna, Brazil. [Melo, B. L. B.; Lemos, L. S. L.] Univ Estadual Santa Cruz PPGGBM, Ilheus, Brazil. [de Souza, J. T.] Univ Fed Reconcavo Bahia, CCAAB, BR-44380000 Cruz Das Almas, Brazil. [Rehner, S. A.] USDA ARS, Systemat Mycol & Microbiol Lab, BARC W, Beltsville, MD 20705 USA. [Solis, K. H.; Suarez, C.] INIAP, Pichilingue, Ecuador. [Hebbar, P. K.] USDA APHIS PPQ, Riverdale, MD 20737 USA. RP Gramacho, KP (reprint author), Cacao Res Ctr, Itabuna, Brazil. EM karina@cepec.gov.br OI Gramacho, Karina/0000-0003-4037-1554 FU CFC/ICCO/BIOMOL; Mars Inc.; PROCITROPICS; CNPq; Fundo de Amparo a Pesquisa da Bahia (FAPESB) [1077/2010]; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) FX The authors thank CFC/ICCO/BIOMOL, Mars Inc. and PROCITROPICS for financially supporting this project and CNPq for providing scholarships to JTS and KPG. BLBM was funded by Fundo de Amparo a Pesquisa da Bahia (FAPESB; grant # 1077/2010) and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES). We thank M. C. Aime for providing isolate C23 in the initial phase of the study. NR 20 TC 1 Z9 1 U1 0 U2 6 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1437-4781 EI 1439-0329 J9 FOREST PATHOL JI Forest Pathol. PD AUG PY 2014 VL 44 IS 4 BP 320 EP 324 DI 10.1111/efp.12103 PG 5 WC Forestry SC Forestry GA AN3LH UT WOS:000340489000008 ER PT J AU Guo, H Wang, XY Gundlach, H Mayer, KFX Peterson, DG Scheffler, BE Chee, PW Paterson, AH AF Guo, Hui Wang, Xiyin Gundlach, Heidrun Mayer, Klaus F. X. Peterson, Daniel G. Scheffler, Brian E. Chee, Peng W. Paterson, Andrew H. TI Extensive and Biased Intergenomic Nonreciprocal DNA Exchanges Shaped a Nascent Polyploid Genome, Gossypium (Cotton) SO GENETICS LA English DT Article ID RIBOSOMAL-RNA GENES; DUPLICATED GENES; TRANSPOSABLE ELEMENTS; MEIOTIC RECOMBINATION; NUCLEOLAR DOMINANCE; ARABIDOPSIS HYBRIDS; BRASSICA-NAPUS; EVOLUTION; WHEAT; CONVERSION AB Genome duplication is thought to be central to the evolution of morphological complexity, and some polyploids enjoy a variety of capabilities that transgress those of their diploid progenitors. Comparison of genomic sequences from several tetraploid (A(t)D(t)) Gossypium species and genotypes with putative diploid A- and D-genome progenitor species revealed that unidirectional DNA exchanges between homeologous chromosomes were the predominant mechanism responsible for allelic differences between the Gossypium tetraploids and their diploid progenitors. Homeologous gene conversion events (HeGCEs) gradually subsided, declining to rates similar to random mutation during radiation of the polyploid into multiple clades and species. Despite occurring in a common nucleus, preservation of HeGCE is asymmetric in the two tetraploid subgenomes. A(t)-to-D-t conversion is far more abundant than the reciprocal, is enriched in heterochromatin, is highly correlated with GC content and transposon distribution, and may silence abundant A-genome-derived retrotransposons. D-t-to-A(t) conversion is abundant in euchromatin and genes, frequently reversing losses of gene function. The long-standing observation that the nonspinnable-fibered D-genome contributes to the superior yield and quality of tetraploid cotton fibers may be explained by accelerated D-t to A(t) conversion during cotton domestication and improvement, increasing dosage of alleles from the spinnable-fibered A-genome. HeGCE may provide an alternative to (rare) reciprocal DNA exchanges between chromosomes in heterochromatin, where genes have approximately five times greater abundance of D-t-to-A(t) conversion than does adjacent intergenic DNA. Spanning exon-to-gene-sized regions, HeGCE is a natural noninvasive means of gene transfer with the precision of transformation, potentially important in genetic improvement of many crop plants. C1 [Guo, Hui; Wang, Xiyin; Paterson, Andrew H.] Univ Georgia, Plant Genome Mapping Lab, Athens, GA 30602 USA. [Guo, Hui; Paterson, Andrew H.] Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA. [Paterson, Andrew H.] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA. [Paterson, Andrew H.] Univ Georgia, Dept Genet, Athens, GA 30602 USA. [Wang, Xiyin] Hebei United Univ, Sch Life Sci, Ctr Genom & Computat Biol, Tangshan 063000, Hebei, Peoples R China. [Wang, Xiyin] Hebei United Univ, Sch Sci, Tangshan 063000, Hebei, Peoples R China. [Gundlach, Heidrun; Mayer, Klaus F. X.] German Res Ctr Environm Hlth, Prot Sequences Inst Bioinformat & Syst Biol, Munich Informat Ctr, D-85764 Neuherberg, Germany. [Peterson, Daniel G.] Mississippi State Univ, Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39762 USA. [Scheffler, Brian E.] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. [Chee, Peng W.] Dept Crop & Soil Sci, Tifton, GA 31794 USA. [Chee, Peng W.] Coastal Plain Expt Stn, Tifton, GA 31794 USA. RP Paterson, AH (reprint author), 111 Riverbend Rd,Rm 228, Athens, GA 30605 USA. EM paterson@plantbio.uga.edu RI Mayer, Klaus/M-7941-2015; OI Mayer, Klaus/0000-0001-6484-1077; Guo, Hui/0000-0001-7830-8564; Scheffler, Brian/0000-0003-1968-8952 FU US National Science Foundation [IIP-0917856, IIP-1127755]; United States Department of Agriculture [Agricultural Research Service (ARS) 58-6402-7-241, 58-6402-1-644, 58-6402-1-645, ARS 6402-21310-003-00]; Bayer Crop Science; Consortium for Plant Biotechnology Research; Cotton, Inc. FX The authors appreciate financial support from the US National Science Foundation (IIP-0917856, IIP-1127755 to A. H. P.); United States Department of Agriculture [Agricultural Research Service (ARS)-58-6402-7-241, 58-6402-1-644, and 58-6402-1-645 to D. G. P. and ARS 6402-21310-003-00 to B. E. S.); Bayer Crop Science; the Consortium for Plant Biotechnology Research; and Cotton, Inc. (A. H. P.). NR 83 TC 12 Z9 12 U1 0 U2 21 PU GENETICS SOCIETY AMERICA PI BETHESDA PA 9650 ROCKVILLE AVE, BETHESDA, MD 20814 USA SN 0016-6731 EI 1943-2631 J9 GENETICS JI Genetics PD AUG PY 2014 VL 197 IS 4 BP 1153 EP U163 DI 10.1534/genetics.114.166124 PG 31 WC Genetics & Heredity SC Genetics & Heredity GA AN5DO UT WOS:000340610100008 PM 24907262 ER PT J AU Hohenadel, MG Thearle, MS Grice, BA Huang, H Dai, MH Tao, YX Hunter, LA Palaguachi, GI Mou, Z Kim, RC Tsang, MM Haack, K Voruganti, VS Cole, SA Butte, NF Comuzzie, AG Muller, YL Baier, LJ Krakoff, J Knowler, WC Yanovski, JA Han, JC AF Hohenadel, M. G. Thearle, M. S. Grice, B. A. Huang, H. Dai, M-H Tao, Y-X Hunter, L. A. Palaguachi, G. I. Mou, Z. Kim, R. C. Tsang, M. M. Haack, K. Voruganti, V. S. Cole, S. A. Butte, N. F. Comuzzie, A. G. Muller, Y. L. Baier, L. J. Krakoff, J. Knowler, W. C. Yanovski, J. A. Han, J. C. TI Brain-derived neurotrophic factor in human subjects with function-altering melanocortin-4 receptor variants SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE BDNF; MC4R; ELISA; SNP; leptin ID AGOUTI-RELATED PROTEIN; PHARMACOLOGICAL CHARACTERIZATION; CHILDHOOD OBESITY; PIMA-INDIANS; FOOD-INTAKE; FACTOR BDNF; MICE; PROOPIOMELANOCORTIN; POLYMORPHISMS; ANTAGONIST AB BACKGROUND: In rodents, hypothalamic brain-derived neurotrophic factor (BDNF) expression appears to be regulated by melanocortin-4 receptor (MC4R) activity. The impact of MC4R genetic variation on circulating BDNF in humans is unknown. OBJECTIVE: The objective of this study is to compare BDNF concentrations of subjects with loss-of-function (LOF) and gain-of-function (GOF) MC4R variants with those of controls with common sequence MC4R. METHODS: Circulating BDNF was measured in two cohorts with known MC4R sequence: 148 subjects of Pima Indian heritage ((mean +/- s.d.): age, 15.7 +/- 6.5 years; body mass index z-scores (BMI-Z), 1.63 +/- 1.03) and 69 subjects of Hispanic heritage (10.8 +/- 3.6 years; BMI-Z, 1.57 +/- 1.07). MC4R variants were characterized in vitro by cell surface expression, receptor binding and cyclic AMP response after agonist administration. BDNF single-nucleotide polymorphisms (SNPs) rs12291186, rs6265 and rs7124442 were also genotyped. RESULTS: In the Pima cohort, no significant differences in serum BDNF was observed for 43 LOF subjects versus 65 LOF-matched controls (age, sex and BMI matched; P = 0.29) or 20 GOF subjects versus 20 GOF-matched controls (P = 0.40). Serum BDNF was significantly associated with genotype for BDNF rs12291186 (P = 0.006) and rs6265 (P = 0.009), but not rs7124442 (P = 0.99); BDNF SNPs did not interact with MC4R status to predict serum BDNF. In the Hispanic cohort, plasma BDNF was not significantly different among 21 LOF subjects, 20 GOF subjects and 28 controls (P = 0.79); plasma BDNF was not predicted by BDNF genotype or BDNF-x-MC4R genotype interaction. CONCLUSIONS: Circulating BDNF concentrations were not significantly associated with MC4R functional status, suggesting that peripheral BDNF does not directly reflect hypothalamic BDNF secretion and/or that MC4R signaling is not a significant regulator of the bulk of BDNF expression in humans. C1 [Hohenadel, M. G.; Thearle, M. S.; Grice, B. A.; Muller, Y. L.; Baier, L. J.; Krakoff, J.; Knowler, W. C.] NIDDK, NIH, Phoenix, AZ USA. [Huang, H.; Dai, M-H; Tao, Y-X] Auburn Univ, Coll Vet Med, Dept Anat Physiol & Pharmacol, Auburn, AL 36849 USA. [Hunter, L. A.; Palaguachi, G. I.; Mou, Z.; Kim, R. C.; Tsang, M. M.; Han, J. C.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Metab & Neuroendocrinol, NIH, Bethesda, MD 20892 USA. [Hunter, L. A.; Palaguachi, G. I.; Mou, Z.; Kim, R. C.; Tsang, M. M.; Yanovski, J. A.; Han, J. C.] Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Sect Growth & Obes, NIH, Bethesda, MD 20892 USA. [Haack, K.; Voruganti, V. S.; Cole, S. A.; Comuzzie, A. G.] Texas Biomed Res Inst, Dept Genet, San Antonio, TX USA. [Butte, N. F.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Han, JC (reprint author), Eunice Kennedy Shriver Natl Inst Child Hlth & Hum, Unit Metab & Neuroendocrinol, NIH, Bldg 10-CRC,Room 1-3330,MSC 1103,10 Ctr Dr, Bethesda, MD 20892 USA. EM hanjo@mail.nih.gov RI Huang, Hui/E-3030-2015; OI Huang, Hui/0000-0003-2958-257X; Tao, Ya-Xiong/0000-0003-4737-749X FU International Hyperphagia Conference Best Idea Grant from the Prader-Willi Syndrome Association (USA); NIH Intramural Research Program of NICHD; NIH Intramural Research Program of NIDDK FX Funding for this study was provided by the International Hyperphagia Conference Best Idea Grant from the Prader-Willi Syndrome Association (USA) (JCH and JAY), by the NIH Intramural Research Programs of NICHD and NIDDK, American Diabetes Association grant 1-12-BS212 (Y-XT), NIH (R01 DK59264 and R01 DK080457 (NFB), and C06 RR013556 and C06 RR017515) and USDA/ARS under Cooperative Agreement 58-6250-51000-037. JK and JAY are Commissioned Officers of the United States Public Health Service. Clinical Trials Registry: Subjects in the Pima cohort were enrolled in observational, non-interventional studies, registered as NCT00339482. Subjects in the Hispanic cohort were enrolled in observational, non-interventional studies. NR 39 TC 5 Z9 5 U1 0 U2 3 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 EI 1476-5497 J9 INT J OBESITY JI Int. J. Obes. PD AUG PY 2014 VL 38 IS 8 BP 1068 EP 1074 DI 10.1038/ijo.2013.221 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AN5XV UT WOS:000340666900007 PM 24276017 ER PT J AU Bandin, C Martinez-Nicolas, A Ordovas, JM Madrid, JA Garaulet, M AF Bandin, C. Martinez-Nicolas, A. Ordovas, J. M. Madrid, J. A. Garaulet, M. TI Circadian rhythmicity as a predictor of weight-loss effectiveness SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE circadian rhythm; weight loss; treatment efficacy; low and high responders ID REST-ACTIVITY RHYTHMS; WRIST TEMPERATURE; LOSS MAINTENANCE; OBESITY; SLEEP; WOMEN; ASSOCIATIONS; CROSSTALK; PROGRAM; DISEASE AB OBJECTIVES: Some of the major challenges associated with successful dietary weight management include the identification of individuals not responsive to specific interventions. The aim was to investigate the potential relationship between weight loss and circadian rhythmicity, using wrist temperature and actimetry measurements, in women undergoing a weight-loss program, in order to assess whether circadian rhythmicity could be a marker of weight-loss effectiveness. METHODS: Participants were 85 overweight and obese women (body mass index, BMI: 30.24 +/- 4.95 kg m(-2)) subjected to a weight-reduction program. Efficacy of the treatment was defined as total weight loss, percentage of initial weight and weekly weight loss rates. Circadian rhythmicity in wrist temperature motor activity and position were analyzed using different sensors. RESULTS: Lower weight loss was related with a more flattened pattern measured as amplitude from cosinor (r = 0.235, P = 0.032), a higher fragmentation of rhythms determined by higher intradaily variability (IV) (r = -0.339, P = 0.002), and an impaired wrist temperature circadian rhythm determined by the means of Circadian Function Index (r = 0.228, P = 0.038). Further analyses showed that low responders displayed lower amplitude (0.71 +/- 0.36 versus 1.24 +/- 0.62, P = 0.036) and higher fragmentation of the circadian rhythm (0.24 +/- 0.11 versus 0.15 +/- 0.07, P = 0.043) than high responders. Whereas we did not find significant differences in total activity rates between high responders and low responders, we found significant differences for the mean values of body position for high responders (39.12 +/- 3.791) as compared with low responder women (35.31 +/- 2.53 degrees, P = 0.01). CONCLUSIONS: Circadian rhythms at the beginning of the treatment are good predictors of future weight loss. Further treatment should consider chronobiological aspects to diagnose obesity and effectiveness of treatments. C1 [Bandin, C.; Martinez-Nicolas, A.; Madrid, J. A.; Garaulet, M.] Univ Murcia, Fac Biol, Dept Physiol, E-30100 Murcia, Spain. [Ordovas, J. M.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Ordovas, J. M.] Ctr Nacl Invest Cardiovasc, Dept Epidemiol, Madrid, Spain. [Ordovas, J. M.] Madrid Inst Adv Studies Food, IMDEA FOOD, Madrid, Spain. RP Garaulet, M (reprint author), Univ Murcia, Fac Biol, Dept Physiol, Campus Espinardo, E-30100 Murcia, Spain. EM garaulet@um.es RI Martinez-Nicolas, Antonio/A-7560-2012 OI Martinez-Nicolas, Antonio/0000-0002-8213-3370 NR 32 TC 13 Z9 13 U1 1 U2 10 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 EI 1476-5497 J9 INT J OBESITY JI Int. J. Obes. PD AUG PY 2014 VL 38 IS 8 BP 1083 EP 1088 DI 10.1038/ijo.2013.211 PG 6 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AN5XV UT WOS:000340666900009 PM 24232497 ER PT J AU Liu, YQ AF Liu, Yongqiang TI A Regression Model for Smoke Plume Rise of Prescribed Fires Using Meteorological Conditions SO JOURNAL OF APPLIED METEOROLOGY AND CLIMATOLOGY LA English DT Article ID UNITED-STATES; SIMULATION; DAYSMOKE AB Smoke plume rise is an important factor for smoke transport and air quality impact modeling. This study provides a practical tool for estimating plume rise of prescribed fires. A regression model was developed on the basis of observed smoke plume rise for 20 prescribed fires in the southeastern United States. The independent variables include surface wind, air temperature, fuel moisture, and atmospheric planetary boundary layer (PBL) height. The first three variables were obtained from the Remote Automatic Weather Stations, most of which are installed in locations where they can monitor local fire danger and are easily accessed by fire managers. The PBL height was simulated with the Weather Research and Forecasting Model. The confidence and validation analyses indicate that the regression model is significant at the 95% confidence level and able to predict hourly values and the average smoke plume rise during a burn, respectively. The prediction of the average smoke plume rise shows larger skills. The model also shows improved skills over two extensively used empirical models for the prescribed burn cases examined in this study, suggesting that it may have the potential to improve smoke plume rise and air quality modeling for prescribed burns. The regression model, however, tends to underestimate large plume rise values and overestimate small ones. A suite of alternative regression models was also provided, one of which can be used when no PBL information is available. C1 US Forest Serv, Ctr Forest Disturbance Sci, USDA, Athens, GA 30602 USA. RP Liu, YQ (reprint author), US Forest Serv, Ctr Forest Disturbance Sci, USDA, 320 Green St, Athens, GA 30602 USA. EM yliu@fs.fed.us FU Joint Fire Science Program [JFSP 081606, JFSP 11172, NIFC2013-35100-20516] FX The author thanks Sanjeeb Bhoi for conducting WRF simulations, colleagues and field managers for collecting the ceilometer measurements, and four reviewers for their constructive comments and suggestions. This study was supported by the Joint Fire Science Program under Agreement JFSP 081606, JFSP 11172, and NIFC2013-35100-20516. The National Center for Atmospheric Research (NCAR) Command Language (NCL) was used for the regression analysis. NR 21 TC 0 Z9 0 U1 1 U2 10 PU AMER METEOROLOGICAL SOC PI BOSTON PA 45 BEACON ST, BOSTON, MA 02108-3693 USA SN 1558-8424 EI 1558-8432 J9 J APPL METEOROL CLIM JI J. Appl. Meteorol. Climatol. PD AUG PY 2014 VL 53 IS 8 BP 1961 EP 1975 DI 10.1175/JAMC-D-13-0114.1 PG 15 WC Meteorology & Atmospheric Sciences SC Meteorology & Atmospheric Sciences GA AN3TS UT WOS:000340512200008 ER PT J AU Dearfield, KL Hoelzer, K Kause, JR AF Dearfield, Kerry L. Hoelzer, Karin Kause, Janell R. TI Review of Various Approaches for Assessing Public Health Risks in Regulatory Decision Making: Choosing the Right Approach for the Problem SO JOURNAL OF FOOD PROTECTION LA English DT Article ID UNITED-STATES; FOOD SAFETY; PRODUCTS; EXPOSURE; ILLNESS; TRADE AB Stakeholders in the public health risk analysis community can possess differing opinions about what is meant by "conduct a risk assessment." In reality, there is no one-size-fits-all risk assessment that can address all public health issues, problems, and regulatory needs. Although several international and national organizations (e.g., Codex Alimentarius Commission, Office International des Epizooties, Food and Agricultural Organization, World Health Organization, National Research Council, and European Food Safety Authority) have addressed this issue, confusion remains. The type and complexity of a risk assessment must reflect the risk management needs to appropriately inform a regulatory or nonregulatory decision, i.e., a risk assessment is ideally "fit for purpose" and directly applicable to risk management issues of concern. Frequently however, there is a lack of understanding by those not completely familiar with risk assessment regarding the specific utility of different approaches for assessing public health risks. This unfamiliarity can unduly hamper the acceptance of risk assessment results by risk managers and may reduce the usefulness of such results for guiding public health policies, practices, and operations. Differences in interpretation of risk assessment terminology further complicate effective communication among risk assessors, risk managers, and stakeholders. This article provides an overview of the types of risk assessments commonly conducted, with examples primarily from the food and agricultural sectors, and a discussion of the utility and limitations of these specific approaches for assessing public health risks. Clarification of the risk management issues and corresponding risk assessment design needs during the formative stages of the risk analysis process is a key step for ensuring that the most appropriate assessment of risk is developed and used to guide risk management decisions. C1 [Dearfield, Kerry L.; Kause, Janell R.] US Food Safety & Inspect Serv, USDA, Off Publ Hlth Sci, Washington, DC 20250 USA. [Hoelzer, Karin] US FDA, Ctr Food Safety & Appl Nutr, College Pk, MD 20740 USA. RP Dearfield, KL (reprint author), US Food Safety & Inspect Serv, USDA, Off Publ Hlth Sci, Washington, DC 20250 USA. EM kerry.dearfield@fsis.usda.gov FU Center for Food Safety and Applied Nutrition FX This work was supported in part by appointments to the Research Participation Program at the Center for Food Safety and Applied Nutrition administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy and the U.S. Food and Drug Administration. NR 79 TC 4 Z9 4 U1 1 U2 16 PU INT ASSOC FOOD PROTECTION PI DES MOINES PA 6200 AURORA AVE SUITE 200W, DES MOINES, IA 50322-2863 USA SN 0362-028X EI 1944-9097 J9 J FOOD PROTECT JI J. Food Prot. PD AUG PY 2014 VL 77 IS 8 BP 1428 EP 1440 DI 10.4315/0362-028X.JFP-14-046 PG 13 WC Biotechnology & Applied Microbiology; Food Science & Technology SC Biotechnology & Applied Microbiology; Food Science & Technology GA AM6YR UT WOS:000340012400025 PM 25198609 ER PT J AU Nemec, KT AF Nemec, Kristine T. TI Tallgrass prairie ants: their species composition, ecological roles, and response to management SO JOURNAL OF INSECT CONSERVATION LA English DT Review DE Tallgrass prairie; Grassland; Ants; Formicidae; Restoration ID IMPORTED FIRE ANT; SOLENOPSIS-INVICTA HYMENOPTERA; NORTH-AMERICA; RESTORATION SUCCESS; FUNCTIONAL-GROUPS; SEED PREDATION; GRASSLAND RESTORATION; ARTHROPOD-COMMUNITY; ECOSYSTEM ENGINEERS; FORMICA-OBSCURIPES AB Ants are highly influential organisms in terrestrial ecosystems, including the tallgrass prairie, one of the most endangered ecosystems in North America. Through their tunneling, ants affect soil properties and resource availability for animals and plants. Ants also have important ecological roles as consumers of plant tissue and seeds. In the last several decades, various organizations, agencies, and agricultural producers have attempted to create wildlife habitat or reduce soil erosion by seeding thousands of hectares of bare cropland in the central United States with tallgrass prairie seed mixes. Although initially, monitoring of these restorations and of unplowed prairie remnants focused on plants and birds, in recent years the response of invertebrates such as ants has increasingly been the subject of research. An understanding of tallgrass prairie ant communities can help land managers and scientists better monitor the ecological condition of tallgrass prairie and guide management and restoration efforts. Here I review our current knowledge of ant species found within tallgrass prairie, their ecological roles, and their response to management. C1 ARS, North Cent Agr Res Lab, USDA, Brookings, SD 57006 USA. RP Nemec, KT (reprint author), ARS, North Cent Agr Res Lab, USDA, 2923 Medary Ave, Brookings, SD 57006 USA. EM kristinenemec14@gmail.com NR 125 TC 2 Z9 3 U1 5 U2 45 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1366-638X EI 1572-9753 J9 J INSECT CONSERV JI J. Insect Conserv. PD AUG PY 2014 VL 18 IS 4 BP 509 EP 521 DI 10.1007/s10841-014-9656-2 PG 13 WC Biodiversity Conservation; Entomology SC Biodiversity & Conservation; Entomology GA AN2XT UT WOS:000340450000001 ER PT J AU Pitts-Singer, TL Cane, JH Trostle, G AF Pitts-Singer, Theresa L. Cane, James H. Trostle, Glen TI Progeny of Osmia lignaria from distinct regions differ in developmental phenology and survival under a common thermal regime SO JOURNAL OF INSECT PHYSIOLOGY LA English DT Article DE Almond pollination; Blue orchard bee; Developmental physiology; Megachilidae; Osmia lignaria; Phenology ID CLIMATE-CHANGE; BIOLOGICAL-CONTROL; PROPINQUA CRESSON; FIELD EXPERIMENTS; EMERGENCE TIME; APPLE ORCHARDS; SOLITARY BEE; HYMENOPTERA; MEGACHILIDAE; TEMPERATURE AB Many insects, including some bees, have extensive subcontinental distributions that can differ in climatic conditions. Within and beyond these distributions, humans intentionally transport beneficial insects, including bees, to non-natal geographic locations. Insects also are experiencing unprecedented climatic change in their resident localities. For solitary bees, we know very little about the adaptive plasticity and geographic variation in developmental physiology that accommodates the different climates experienced within distributional ranges. Osmia lignaria Say (Hymenoptera: Megachilidae) is a widely distributed North American spring-emerging bee being developed as a managed pollinator for tree fruit crops, including almonds. We examined the development and survival of O. lignaria progeny that were descended from populations sourced from southern California, western Washington, and northern Utah, and then were reared together under an hourly and weekly temperature regime simulating those of a California almond-growing region. We found that developmental physiologies of Washington and Utah progeny were generally similar. However, California progeny developed slower, were more metabolically active, and survived better under California conditions than did populations native to regions at higher latitudes. Regardless of geographic origin, cocooned adults managed under prescribed thermal regimes emerged faster and lived longer after wintering. Progeny of parents from different regions exhibited some acclimatory plasticity in developmental phenologies to a novel climatic regime, but overall their responses reflected their geographic origins. This outcome is consistent with their developmental phenologies being largely heritable adaptations to regional climates. (C) Published by Elsevier Ltd. C1 [Pitts-Singer, Theresa L.; Cane, James H.; Trostle, Glen] Utah State Univ, USDA ARS, Pollinating Insects Res Unit, Logan, UT 84322 USA. RP Pitts-Singer, TL (reprint author), Utah State Univ, USDA ARS, Pollinating Insects Res Unit, Logan, UT 84322 USA. EM Theresa.Pitts-Singer@ars.usda.gov; Jim.Cane@ars.usda.gov; Glentrostle@gmail.com FU Almond Board of California FX For partial funding of this project, we thank The Almond Board of California, which was not involved in the experimental design, the writing of this paper, or the decision to publish this work. We thank ARS technicians Stephanie Miller, Melissa Weber, Katie Swoboda, Shaila Kalaskar, and Ellen Klomps, and Utah State University undergraduates Daniel Sharp and Daniel Young for their invaluable assistance in nest dissections, temperature data preparation, bee development and respirometry data collections, and data entry. We appreciate the conscientious efforts of Sara Goldman-Smith and Steve Peterson for nest retrieval in California and for shipping them to Utah. We also thank AgPollen, LLC, Dan Cummings, and Jimmy Bertagna for allowing us to fly our bees in their orchards. We thank Jordi Bosch, George Yocum, and two anonymous reviewers for their careful reviews and commentaries that greatly improved this manuscript. NR 43 TC 2 Z9 2 U1 3 U2 56 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0022-1910 EI 1879-1611 J9 J INSECT PHYSIOL JI J. Insect Physiol. PD AUG PY 2014 VL 67 BP 9 EP 19 DI 10.1016/j.jinsphys.2014.05.018 PG 11 WC Entomology; Physiology; Zoology SC Entomology; Physiology; Zoology GA AN1FA UT WOS:000340326900002 PM 24879969 ER PT J AU Byrdwell, WC AF Byrdwell, William Craig TI Extract-filter-shoot liquid chromatography with mass spectrometry for the analysis of vitamin D-2 in a powdered supplement capsule and standard reference material 3280 SO JOURNAL OF SEPARATION SCIENCE LA English DT Article DE Cholecalciferol; Ergocalciferol; Vitamin D ID CARDIOVASCULAR-DISEASE; DIETARY-SUPPLEMENT; LIPID EXTRACTION; RISK; TRIACYLGLYCEROLS; PURIFICATION; SOLVENT; CANCER; HEALTH; FOODS AB An "extract-filter-shoot" method for the analysis of vitamin D-2, ergocalciferol, in a dry powdered dietary supplement capsule containing rice flour excipient and in a National Institute of Standards and Technology standard reference material 3280 is reported. Quantification of vitamin D-2 was done by atmospheric pressure chemical ionization mass spectrometry using selected ion monitoring, two transitions of selected reaction monitoring, and extracted ion chromatograms from full scans. UV detection was used for the quantification of Vitamin D-2 in the dry powder capsule, whereas interfering species rendered UV detection unreliable for standard reference material 3280. Average values for standard reference material 3280 ranged from 8.27 +/- 0.58 to 8.33 +/- 0.57 mu g/g using internal standard calibration and response factor approaches, compared to the previous National Institute of Standards and Technology internal value for vitamin D2 of 8.78 +/- 0.11 mu g/g, and the recently updated reference value of 8.6 +/- 2.6 mu g/g. The powdered supplement capsule was found to contain 28.19 +/- 0.35 to 28.67 +/- 0.90 mu g/capsule for a capsule labeled to contain 25.00 mu g. The triacylglycerol composition of the rice flour excipient in the powdered supplement capsule determined by atmospheric pressure chemical ionization mass spectrometry is also reported. C1 ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, USDA, Beltsville, MD 20705 USA. RP Byrdwell, WC (reprint author), ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, USDA, 10300 Baltimore Ave,Bldg 161, Beltsville, MD 20705 USA. EM C.Byrdwell@ars.usda.gov OI Byrdwell, William/0000-0001-8241-428X FU USDA Agricultural Research Service FX This work was supported by the USDA Agricultural Research Service. The mention or use of specific products or brands does not represent or imply endorsement by the USDA. The work of Brian Nies of Morpho Detection (formerly Syagen Technologies) to refurbish the APPI source for the LCQ Deca XP instrument is gratefully acknowledged. The work of Dr. Robert Goldschmidt to perform UV, CAD, and ELSD peak integration is gratefully acknowledged. NR 30 TC 2 Z9 2 U1 2 U2 4 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 1615-9306 EI 1615-9314 J9 J SEP SCI JI J. Sep. Sci. PD AUG PY 2014 VL 37 IS 16 BP 2095 EP 2110 DI 10.1002/jssc.201400234 PG 16 WC Chemistry, Analytical SC Chemistry GA AN4KJ UT WOS:000340556300002 PM 24854739 ER PT J AU Grabowski, PP Morris, GP Casler, MD Borevitz, JO AF Grabowski, Paul P. Morris, Geoffrey P. Casler, Michael D. Borevitz, Justin O. TI Population genomic variation reveals roles of history, adaptation and ploidy in switchgrass SO MOLECULAR ECOLOGY LA English DT Article DE ecotypes; genotyping-by-sequencing; perennial grass; polyploidy ID PANICUM-VIRGATUM; EVOLUTIONARY CONSEQUENCES; ARABIDOPSIS-THALIANA; FLOWERING PLANTS; LOWLAND SWITCHGRASS; HYBRID SPECIATION; WIDE ASSOCIATION; NORTH-AMERICA; DIVERGENCE; POLYPLOIDY AB Geographic patterns of genetic variation are shaped by multiple evolutionary processes, including genetic drift, migration and natural selection. Switchgrass (Panicum virgatum L.) has strong genetic and adaptive differentiation despite life history characteristics that promote high levels of gene flow and can homogenize intraspecific differences, such as wind-pollination and self-incompatibility. To better understand how historical and contemporary factors shape variation in switchgrass, we use genotyping-by-sequencing to characterize switchgrass from across its range at 98042 SNPs. Population structuring reflects biogeographic and ploidy differences within and between switchgrass ecotypes and indicates that biogeographic history, ploidy incompatibilities and differential adaptation each have important roles in shaping ecotypic differentiation in switchgrass. At one extreme, we determine that two Panicum taxa are not separate species but are actually conspecific, ecologically divergent types of switchgrass adapted to the extreme conditions of coastal sand dune habitats. Conversely, we identify natural hybrids among lowland and upland ecotypes and visualize their genome-wide patterns of admixture. Furthermore, we determine that genetic differentiation between primarily tetraploid and octoploid lineages is not caused solely by ploidy differences. Rather, genetic diversity in primarily octoploid lineages is consistent with a history of admixture. This suggests that polyploidy in switchgrass is promoted by admixture of diverged lineages, which may be important for maintaining genetic differentiation between switchgrass ecotypes where they are sympatric. These results provide new insights into the mechanisms shaping variation in widespread species and provide a foundation for dissecting the genetic basis of adaptation in switchgrass. C1 [Grabowski, Paul P.; Casler, Michael D.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Grabowski, Paul P.; Morris, Geoffrey P.; Borevitz, Justin O.] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA. [Morris, Geoffrey P.] Kansas State Univ, Dept Agron, Throckmorton Plant Sci Ctr 3004, Manhattan, KS 66506 USA. [Borevitz, Justin O.] Australian Natl Univ, Res Sch Biol, Canberra, ACT 0200, Australia. RP Grabowski, PP (reprint author), USDA ARS, US Dairy Forage Res Ctr, 1925 Linden Dr, Madison, WI 53706 USA. EM grabowsp@uchicago.edu RI Borevitz, Justin/B-5423-2012; OI Morris, Geoffrey/0000-0002-3067-3359 FU National Institutes of Health [T32 GM007197] FX We thank Indiana Dunes State Park and Indiana Department of Natural Resources for help with sampling, David Lowry for flow cytometry data, Robert Elshire for discussions about GBS library preparation, Jonathan Pritchard for analysis advice, Nina Noah for laboratory assistance and three anonymous reviewers for helpful suggestions. P. P. G. was partially supported by National Institutes of Health Training Grant T32 GM007197. NR 61 TC 9 Z9 9 U1 12 U2 70 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0962-1083 EI 1365-294X J9 MOL ECOL JI Mol. Ecol. PD AUG PY 2014 VL 23 IS 16 BP 4059 EP 4073 DI 10.1111/mec.12845 PG 15 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA AN2MO UT WOS:000340419800011 PM 24962137 ER PT J AU Quattrin, T Roemmich, JN Paluch, R Yu, J Epstein, LH Ecker, MA AF Quattrin, Teresa Roemmich, James N. Paluch, Rocco Yu, Jihnhee Epstein, Leonard H. Ecker, Michelle A. TI Treatment Outcomes of Overweight Children and Parents in the Medical Home SO PEDIATRICS LA English DT Article DE overweight; obesity; patient centered medical home; percent over BMI; preschool children; expert committee recommendations ID BEHAVIORAL OBESITY TREATMENT; WEIGHT CONTROL; CHILDHOOD OBESITY; PRIMARY-CARE; METABOLIC SYNDROME; ADULT OBESITY; ADOLESCENTS; MAINTENANCE; INTERVENTIONS; TRIAL AB OBJECTIVE: To test in the primary care setting the short-and long-term efficacy of a behavioral intervention that simultaneously targeted an overweight child and parent versus an information control (IC) targeting weight control only in the child. METHODS: Two-to 5-year-old children who had BMI >= 85th percentile and an overweight parent (BMI >25 kg/m(2)) were randomized to Intervention or IC, both receiving diet and activity education over 12 months (13 sessions) followed by 12-month follow-up (3 sessions). Parents in the Intervention group were also targeted for weight control and received behavioral intervention. Pediatricians in 4 practices enrolled their patients with the assistance of embedded recruiters (Practice Enhancement Assistants) who assisted with treatment too. RESULTS: A total of 96 of the 105 children randomized (Intervention n = 46; IC n = 50) started the program and had data at baseline. Children in the Intervention experienced greater reductions in percent over BMI (group X months; P = .002) and z-BMI (group X months; P < 0.001) compared with IC throughout treatment and follow-up. Greater BMI reduction was observed over time for parents in the Intervention compared with IC (P < .001) throughout treatment and follow-up. Child weight changes were correlated with parent weight changes at 12 and 24 months (r = 0.38 and 0.26; P < .001 and P = .03). CONCLUSIONS: Concurrently targeting preschool-aged overweight and obese youth and their parents in primary care with behavioral intervention results in greater decreases in child percent over BMI, z-BMI, and parent BMI compared with IC. The difference between Intervention and IC persists after 12 months of follow-up. C1 [Quattrin, Teresa; Roemmich, James N.; Paluch, Rocco; Epstein, Leonard H.; Ecker, Michelle A.] SUNY Buffalo, Women & Childrens Hosp, Dept Pediat, Buffalo, NY 14260 USA. [Roemmich, James N.] USDA ARS, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Yu, Jihnhee] SUNY Buffalo, Dept Biostat, Buffalo, NY 14260 USA. RP Quattrin, T (reprint author), Women & Childrens Hosp Buffalo, Div Pediat Endocrinol, 239 Bryant St, Buffalo, NY 14222 USA. EM tquattrin@upa.chob.edu FU National Institutes of Health [1R01HD053773-01]; National Institutes of Health (NIH); Principal Investigator; Teresa Quattrin FX Supported by National Institutes of Health grant 1R01HD053773-01, Principal Investigator, Teresa Quattrin. Funded by the National Institutes of Health (NIH). NR 48 TC 15 Z9 15 U1 0 U2 2 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 EI 1098-4275 J9 PEDIATRICS JI Pediatrics PD AUG PY 2014 VL 134 IS 2 BP 290 EP 297 DI 10.1542/peds.2013-4084 PG 8 WC Pediatrics SC Pediatrics GA AN0HS UT WOS:000340266000055 PM 25049340 ER PT J AU Huda, MN Lewis, Z Kalanetra, KM Rashid, M Ahmad, SM Raqib, R Qadri, F Underwood, MA Mills, DA Stephensen, CB AF Huda, M. Nazmul Lewis, Zachery Kalanetra, Karen M. Rashid, Mamunur Ahmad, Shaikh M. Raqib, Rubhana Qadri, Firdausi Underwood, Mark A. Mills, David A. Stephensen, Charles B. TI Stool Microbiota and Vaccine Responses of Infants SO PEDIATRICS LA English DT Article DE vaccine; intestinal; microbiota; Bifidobacterium; Actinobacteria; Proteobacteria; Bangladesh; T lymphocyte; antibody; polio; tetanus; tuberculosis; hepatitis ID INTESTINAL MICROBIOTA; GUT MICROBIOTA; IMMUNE-RESPONSES; PROBIOTIC BACTERIA; COLONIZATION; MATURATION; DISEASE; SYSTEM; DIVERSITY; EVOLUTION AB OBJECTIVE: Oral vaccine efficacy is low in less-developed countries, perhaps due to intestinal dysbiosis. This study determined if stool microbiota composition predicted infant oral and parenteral vaccine responses. METHODS: The stool microbiota of 48 Bangladeshi infants was characterized at 6, 11, and 15 weeks of age by amplification and sequencing of the 16S ribosomal RNA gene V4 region and by Bifidobacterium-specific, quantitative polymerase chain reaction. Responses to oral polio virus (OPV), bacille Calmette-Gu rin (BCG), tetanus toxoid (TT), and hepatitis B virus vaccines were measured at 15 weeks by using vaccine-specific T-cell proliferation for all vaccines, the delayed-type hypersensitivity skin-test response for BCG, and immunoglobulin G responses using the antibody in lymphocyte supernatant method for OPV, TT, and hepatitis B virus. Thymic index (TI) was measured by ultrasound. RESULTS: Actinobacteria (predominantly Bifidobacterium longum subspecies infantis) dominated the stool microbiota, with Proteobacteria and Bacteroidetes increasing by 15 weeks. Actinobacteria abundance was positively associated with T-cell responses to BCG, OPV, and TT; with the delayed-type hypersensitivity response; with immunoglobulin G responses; and with TI. B longum subspecies infantis correlated positively with TI and several vaccine responses. Bacterial diversity and abundance of Enterobacteriales, Pseudomonadales, and Clostridiales were associated with neutrophilia and lower vaccine responses. CONCLUSIONS: Bifidobacterium predominance may enhance thymic development and responses to both oral and parenteral vaccines early in infancy, whereas deviation from this pattern, resulting in greater bacterial diversity, may cause systemic inflammation (neutrophilia) and lower vaccine responses. Vaccine responsiveness may be improved by promoting intestinal bifidobacteria and minimizing dysbiosis early in infancy. C1 [Huda, M. Nazmul; Stephensen, Charles B.] USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Huda, M. Nazmul; Rashid, Mamunur; Ahmad, Shaikh M.; Raqib, Rubhana; Qadri, Firdausi] Int Ctr Diarrhoeal Dis Res, Dhaka 1000, Bangladesh. [Lewis, Zachery; Kalanetra, Karen M.; Mills, David A.] Univ Calif Davis, Dept Viticulture & Enol, Davis, CA 95616 USA. [Underwood, Mark A.] Univ Calif Davis, Dept Pediat, Davis, CA 95616 USA. RP Stephensen, CB (reprint author), Univ Calif Davis, USDA, Western Human Nutr Res Ctr, 430 West Hlth Sci Dr, Davis, CA 95616 USA. EM charles.stephensen@ars.usda.gov RI Christ Angotti, Melisa/H-6077-2016; Beletini, Lucimara Fatima/H-6083-2016; OI Raqib, Rubhana/0000-0002-8116-4511 FU National Center for Research Resources [UL1 RR024146]; US Department of Agriculture-Agricultural Research Service [5306-51530-018-00]; World Health Organization [2010168947]; Bill and Melinda Gates Foundation; National Institutes of Health (NIH) FX This work was funded by grant UL1 RR024146 from the National Center for Research Resources, US Department of Agriculture-Agricultural Research Service project 5306-51530-018-00, and World Health Organization project 2010168947, which was funded by the Bill and Melinda Gates Foundation. Funded by the National Institutes of Health (NIH). NR 45 TC 40 Z9 42 U1 5 U2 24 PU AMER ACAD PEDIATRICS PI ELK GROVE VILLAGE PA 141 NORTH-WEST POINT BLVD,, ELK GROVE VILLAGE, IL 60007-1098 USA SN 0031-4005 EI 1098-4275 J9 PEDIATRICS JI Pediatrics PD AUG PY 2014 VL 134 IS 2 BP E362 EP E372 DI 10.1542/peds.2013-3937 PG 11 WC Pediatrics SC Pediatrics GA AN0HS UT WOS:000340266000006 PM 25002669 ER PT J AU Schmelz, EA Huffaker, A Sims, JW Christensen, SA Lu, X Okada, K Peters, RJ AF Schmelz, Eric A. Huffaker, Alisa Sims, James W. Christensen, Shawn A. Lu, Xuan Okada, Kazunori Peters, Reuben J. TI Biosynthesis, elicitation and roles of monocot terpenoid phytoalexins SO PLANT JOURNAL LA English DT Article DE momilactone; phytocassane; oryzalexin; kauralexin; zealexin; herbivory; Oryza sativa; Zea mays ID CULTURED RICE CELLS; COPALYL DIPHOSPHATE SYNTHASES; ACTIVATED PROTEIN-KINASE; BLIGHT-RESISTANT CULTIVAR; ELICITOR-INDUCED ACTIVATION; ETHYLENE-INDUCING XYLANASE; WHITE-BACKED PLANTHOPPER; DEFENSE GENE-EXPRESSION; ENT-KAURENE OXIDASE; DISEASE-RESISTANCE AB A long-standing goal in plant research is to optimize the protective function of biochemical agents that impede pest and pathogen attack. Nearly 40 years ago, pathogen-inducible diterpenoid production was described in rice, and these compounds were shown to function as antimicrobial phytoalexins. Using rice and maize as examples, we discuss recent advances in the discovery, biosynthesis, elicitation and functional characterization of monocot terpenoid phytoalexins. The recent expansion of known terpenoid phytoalexins now includes not only the labdane-related diterpenoid superfamily but also casbane-type diterpenoids and beta-macrocarpene-derived sequiterpenoids. Biochemical approaches have been used to pair pathway precursors and end products with cognate biosynthetic genes. The number of predicted terpenoid phytoalexins is expanding through advances in cereal genome annotation and terpene synthase characterization that likewise enable discoveries outside the Poaceae. At the cellular level, conclusive evidence now exists for multiple plant receptors of fungal-derived chitin elicitors, phosphorylation of membrane-associated signaling complexes, activation of mitogen-activated protein kinase, involvement of phytohormone signals, and the existence of transcription factors that mediate the expression of phytoalexin biosynthetic genes and subsequent accumulation of pathway end products. Elicited production of terpenoid phytoalexins exhibit additional biological functions, including root exudate-mediated allelopathy and insect antifeedant activity. Such findings have encouraged consideration of additional interactions that blur traditionally discrete phytoalexin classifications. The establishment of mutant collections and increasing ease of genetic transformation assists critical examination of further biological roles. Future research directions include examination of terpenoid phytoalexin precursors and end products as potential signals mediating plant physiological processes. C1 [Schmelz, Eric A.; Huffaker, Alisa; Sims, James W.; Christensen, Shawn A.] ARS, Ctr Med Agr & Vet Entomol, USDA, Chem Res Unit, Gainesville, FL 32608 USA. [Lu, Xuan; Peters, Reuben J.] Iowa State Univ, Dept Biochem Biophys & Mol Biol, Ames, IA 50011 USA. [Okada, Kazunori] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan. RP Schmelz, EA (reprint author), ARS, Ctr Med Agr & Vet Entomol, USDA, Chem Res Unit, Gainesville, FL 32608 USA. EM Eric.Schmelz@ars.usda.gov RI Peters, Reuben/D-1196-2010; OI Peters, Reuben/0000-0003-4691-8477; Schmelz, Eric/0000-0002-2837-734X FU US Department of Agriculture Agricultural Research Service [6615-21000-010-00, 6615-22000-027-00]; National Science Foundation Division of Integrative Organismal Systems Competitive Award [1139329] FX This work was supported by US Department of Agriculture Agricultural Research Service Projects 6615-21000-010-00 and 6615-22000-027-00, and by National Science Foundation Division of Integrative Organismal Systems Competitive Award 1139329. NR 191 TC 43 Z9 45 U1 11 U2 127 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD AUG PY 2014 VL 79 IS 4 SI SI BP 659 EP 678 DI 10.1111/tpj.12436 PG 20 WC Plant Sciences SC Plant Sciences GA AN3PA UT WOS:000340499200010 PM 24450747 ER PT J AU Flores-Ramirez, G Jankovicova, B Bilkova, Z Miernyk, JA Skultety, L AF Flores-Ramirez, Gabriela Jankovicova, Barbora Bilkova, Zuzana Miernyk, Jan A. Skultety, Ludovit TI Identification of Coxiella burnetii surface-exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy SO PROTEOMICS LA English DT Article DE Bioinformatic predictions; Coxiella burnetii; Microbiology; MS identification; Outer membrane proteins; Surface-exposed and cell envelope associated proteins ID OUTER-MEMBRANE-PROTEIN; GRAM-NEGATIVE BACTERIA; MYCOBACTERIUM-TUBERCULOSIS H37RV; TRITON X-114 EXTRACTS; Q-FEVER; ESCHERICHIA-COLI; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; SUBCELLULAR-LOCALIZATION; LEGIONELLA-PNEUMOPHILA; GEL-ELECTROPHORESIS AB The Gram-negative pathogen Coxiella burnetii is an intracellular bacterium that replicates within the phagolysosomal vacuoles of eukaryotic cells. This pathogen can infect a wide range of hosts, and is the causative agent of Q fever in humans. Surface-exposed and cell envelope associated proteins are thought to be important for both pathogenesis and protective immunity. Herein, we propose a complementary strategy consisting of (i) in silico prediction and (ii) inventory of the proteomic composition using three enrichment approaches coupled with protein identification. The efficiency of classical Triton X-114 phase partitioning was compared with two novel procedures; isolation of alkaline proteins by liquid-phase IEF, and cell surface enzymatic shaving using biofunctional magnetic beads. Of the 2026 protein sequences analyzed using seven distinct bioinformatic algorithms, 157 were predicted to be outer membrane proteins (OMP) and/or lipoproteins (LP). Using the three enrichment protocols, we identified 196 nonredundant proteins, including 39 predicted OMP and/or LP, 32 unknown or poorly characterized proteins, and 17 effectors of the Type IV secretion system. We additionally identified eight proteins with moonlighting activities, and several proteins apparently peripherally associated with integral or anchored OMP and/or LP. C1 [Flores-Ramirez, Gabriela; Skultety, Ludovit] Slovak Acad Sci, Inst Virol, Bratislava 84505, Slovakia. [Jankovicova, Barbora; Bilkova, Zuzana] Univ Pardubice, Fac Chem Technol, Dept Biol & Biochem Sci, Pardubice, Czech Republic. [Miernyk, Jan A.] Univ Missouri, ARS, USDA, Plant Genet Res Unit,Dept Biochem, Columbia, SC USA. [Skultety, Ludovit] Acad Sci Czech Republic, Inst Microbiol, Prague, Czech Republic. RP Skultety, L (reprint author), Slovak Acad Sci, Inst Virol, Bratislava 84505, Slovakia. EM viruludo@savba.sk RI Skultety, Ludovit/N-2160-2014 FU Scientific Grant Agency of the Ministry of Education of the Slovak Republic [2/0156/11, 2/0031/11]; Slovak Academy of Sciences; Agency for Research and Development [APVV-0280-12]; Research & Development Operational Programme - ERDF [26240220062]; Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.07/2.3.00/30.0021]; IMIC [RVO61388971] FX The authors acknowledge the excellent technical assistance of M. Benkovicova. Part of the cost of this study was covered by the following grants: 2/0156/11, 2/0031/11 of the Scientific Grant Agency of the Ministry of Education of the Slovak Republic and the Slovak Academy of Sciences, the APVV-0280-12 supported by Agency for Research and Development, and the 26240220062 supported by the Research & Development Operational Programme funded by the ERDF as well as the grant CZ.1.07/2.3.00/30.0021 supported by the Ministry of Education, Youth and Sports of the Czech Republic and the IMIC institutional research concept RVO61388971. NR 96 TC 8 Z9 8 U1 4 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1615-9853 EI 1615-9861 J9 PROTEOMICS JI Proteomics PD AUG PY 2014 VL 14 IS 16 BP 1868 EP 1881 DI 10.1002/pmic.201300338 PG 14 WC Biochemical Research Methods; Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA AN2NH UT WOS:000340422000003 PM 24909302 ER PT J AU Uchimiya, M AF Uchimiya, Minori TI Influence of pH, Ionic Strength, and Multidentate Ligand on the Interaction of Cd-II with Biochars SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Chelating agent; Remediation; Activated carbon; Bioenergy; Biomass ID DIVALENT METAL-IONS; HEAVY-METALS; ACTIVATED CARBON; ORGANIC-MATTER; ADSORPTION; SORPTION; CADMIUM; WATER; PYROLYSIS; OXIDES AB Pyrolysis temperature-dependent changes in pH, ionic strength, and metal ion-coordinating dissolved organic carbon (DOC) control the sorption of Cd-II and other transition metals on biochar. Sorption of Cd-II on 300-700 degrees C pecan shell biochars was strongly pH- and ionic strength-dependent. Equilibrium speciation calculation suggested that CdOH+ and other hydrolysis products were the "reactive" Cd-II species engaging in the surface interaction with biochar. Sorption of Cd-II on 700 degrees C (lowest O:C) biochar progressively increased from pH 3 to 7 and was not affected by the citrate concentration. In contrast, low concentration of citrate ([Cd-II]: [citrate] molar ratios of 4-24) dramatically enhanced Cd-II sorption on lower temperature (300-500 degrees C) biochars; higher citrate concentrations diminished Cd-II sorption. Citrate-enhanced Cd-II sorption was accompanied by (i) a several-fold increase in DOC and (ii) pH buffering near 7 at equilibrium. Carboxyl ligands form an unusually strong hydrogen bond with the surface functional group of biochar having a similar pK(a). Sorbed ligand (citrate) could (i) provide new Cd-II sorption sites on biochar, (ii) buffer pH near 7, and (iii) facilitate the release of higher molecular weight DOC from biochar. C1 ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA. RP Uchimiya, M (reprint author), ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM sophie.uchimiya@ars.usda.gov NR 44 TC 11 Z9 11 U1 10 U2 77 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD AUG PY 2014 VL 2 IS 8 BP 2019 EP 2027 DI 10.1021/sc5002269 PG 9 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA AM6NM UT WOS:000339981900012 ER PT J AU Elkasabi, Y Mullen, CA Boateng, AA AF Elkasabi, Yaseen Mullen, Charles A. Boateng, Akwasi A. TI Distillation and Isolation of Commodity Chemicals from Bio-Oil Made by Tail-Gas Reactive Pyrolysis SO ACS SUSTAINABLE CHEMISTRY & ENGINEERING LA English DT Article DE Bio-oil; Upgrading; Pyrolysis; Distillation; Separations; Process modeling ID BIOMASS; PERSPECTIVE; COLUMNS; FUELS AB Owing to instabilities, very little has been accomplished with regard to simple cost-effective separations of fast pyrolysis bio-oil. However, recent developments in the use of tail-gas reactive pyrolysis (TGRP) (Mullen, C. A.; Boateng, A. A.; Goldberg, N. M. Energy Fuels 2013.) provide higher quality bio-oils that are thermally stable. We used fractional distillation to isolate compounds from bio-oil produced by TGRP. All bio-oils produced from TGRP contained significantly less acid (0.2-4% total), trace amounts of aldehydes, and significantly higher concentrations of hydrocarbons and phenolics (5-20% each). One TGRP bio-oil rich in naphthalenes yielded the greatest mass of distillates (close to 6596). Using atmospheric distillation alone, we recovered the most distillates from another phenol-rich TGRP oil. Compared to traditional bio-oils, distillation of TGRP oils yielded three times more organic compounds; the yield improvement increases to a factor of 10 when the contribution of acetic acid is removed from consideration. Greater deficiencies in distillation yield occur with higher acids content (4 wt % acid). We categorized distillates according to boiling temperature and chemical components: benzene-toluene-xylenes (BTX), styrene, indene, pyridine, phenols, naphthalenes, acetic acid, and fluorene/anthracene. The relatively narrow product distributions allowed for the isolation of pure naphthalene by recrystallization from a naphthalene-rich fraction. Thermodynamic computation of the TGRP distillation profiles correctly predicted the experimental results, except when acid content equaled 4 wt %. The quality of the TGRP bio-oils allows for insertion into existing refineries, as well as the required Modeling and scale-up. C1 [Elkasabi, Yaseen; Mullen, Charles A.; Boateng, Akwasi A.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Elkasabi, Y (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM yaseen.elkasabi@ars.usda.gov FU USDA-NIFA-BRDI [2012-10008-20271] FX We acknowledge Christina Papadimitriou (summer 2013 USDA Pathway scholar from the University of South Carolina) and Kevin Minsker (Drexel University co-op, Fall 2013), who each provided support work for the distillation and characterization experiments. We also acknowledge Craig Einfeldt, who provided support work for the fast pyrolysis experiments, and Anna Pighinelli who provided the eucalyptus bio-oil. The USDA-NIFA-BRDI Grant 2012-10008-20271 is hereby acknowledged. NR 25 TC 15 Z9 15 U1 7 U2 55 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 2168-0485 J9 ACS SUSTAIN CHEM ENG JI ACS Sustain. Chem. Eng. PD AUG PY 2014 VL 2 IS 8 BP 2042 EP 2052 DI 10.1021/sc5002879 PG 11 WC Chemistry, Multidisciplinary; GREEN & SUSTAINABLE SCIENCE & TECHNOLOGY; Engineering, Chemical SC Chemistry; Science & Technology - Other Topics; Engineering GA AM6NM UT WOS:000339981900015 ER PT J AU Steelman, TA Nowell, B Bayoumi, D McCaffrey, S AF Steelman, Toddi A. Nowell, Branda Bayoumi, Deena McCaffrey, Sarah TI Understanding Information Exchange During Disaster Response: Methodological Insights From Infocentric Analysis SO ADMINISTRATION & SOCIETY LA English DT Article DE disasters; information; social network analysis; interorganizational coordination communication; exchange markets; infocentric social network analysis; wildfire ID WILDLAND-URBAN INTERFACE; CRISIS COMMUNICATION; EMERGENCY MANAGEMENT; NETWORK GOVERNANCE; INTERORGANIZATIONAL COORDINATION; RISK; FIRE; COMMUNITIES; SYSTEMS AB We leverage economic theory, network theory, and social network analytical techniques to bring greater conceptual and methodological rigor to understand how information is exchanged during disasters. We ask, "How can information relationships be evaluated more systematically during a disaster response?" "Infocentric analysis"-a term and approach we develop here-can (a) define an information market and information needs, (b) identify suppliers of information and mechanisms for information exchange, (c) map the information exchange network, and (d) diagnose information exchange failures. These steps are essential for describing how information flows, diagnosing complications, and positing solutions to rectify information problems during a disaster. C1 [Steelman, Toddi A.] Univ Saskatchewan, Sch Environm & Sustainabil, Saskatoon, SK S7N 5C8, Canada. [Nowell, Branda] N Carolina State Univ, Raleigh, NC 27695 USA. [Bayoumi, Deena] N Carolina State Univ, Sch Publ & Int Affairs, Raleigh, NC 27695 USA. [McCaffrey, Sarah] US Forest Serv, USDA, No Res Stn, Chicago, IL USA. RP Steelman, TA (reprint author), Univ Saskatchewan, 329 Kirk Hall,117 Sci Pl, Saskatoon, SK S7N 5C8, Canada. EM toddi.steelman@usask.ca NR 50 TC 2 Z9 2 U1 5 U2 29 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0095-3997 EI 1552-3039 J9 ADMIN SOC JI Adm. Soc. PD AUG PY 2014 VL 46 IS 6 BP 707 EP 743 DI 10.1177/0095399712469198 PG 37 WC Public Administration SC Public Administration GA AM8BL UT WOS:000340094100005 ER PT J AU Trozzo, KE Munsell, JF Chamberlain, JL AF Trozzo, Katie E. Munsell, John F. Chamberlain, James L. TI Landowner interest in multifunctional agroforestry Riparian buffers SO AGROFORESTRY SYSTEMS LA English DT Article DE Adoption; Agroforestry; Native fruit and nut trees; Riparian forest buffer; Universal theory of acceptance and use of technology ID INFORMATION-TECHNOLOGY; USER ACCEPTANCE; FOREST OWNERS; SELF-EFFICACY; ADOPTION; PERCEPTIONS; INNOVATIONS; MOTIVATIONS; ATTITUDES; FARMERS AB Adoption of temperate agroforestry practices generally remains limited despite considerable advances in basic science. This study builds on temperate agroforestry adoption research by empirically testing a statistical model of interest in native fruit and nut tree riparian buffers using technology and agroforestry adoption theory. Data were collected in three watersheds in Virginia's ridge and valley region and used to test hypothesized predictors of interest in planting these buffers. Confirmatory factor analysis was used to verify independence of underlying latent measures. Multiple linear regression was used to model interest using the Universal Theory of Acceptance and Use of Technology (UTAUT). A second model that added agroforestry-specific predictors from Pattanayak et al. (Agrofor Syst 57:173-186, 2003) to UTAUT was tested and compared with the first. The first model was robust (Adj R (2) = 0.49) but was improved by adding agroforestry specific predictors (Adj R (2) = 0.57). Model generalizability was confirmed using double cross validation and normality indices. Social influence, risk expectancy, planting experience, performance expectancy, parcel size, and the interaction of gender and risk were significant in the final model. In addition, socioeconomic variables were used to characterize landowners according to their level of interest. Respondents with greater interest were newer owners that have higher incomes and are less active in farming. The result implies that future agroforesters may in large part consist of owners that have recently acquired land and manage their property more extensively with higher discretionary income and multiple objectives in mind. C1 [Trozzo, Katie E.; Munsell, John F.] Virginia Tech, Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Chamberlain, James L.] US Forest Serv, USDA, Southern Res Stn, Blacksburg, VA 24060 USA. RP Trozzo, KE (reprint author), Virginia Tech, Forest Resources & Environm Conservat, 242 Cheatham Hall 0324, Blacksburg, VA 24061 USA. EM ketrozzo@vt.edu FU United States Department of Agriculture National Agroforestry Center FX The authors would like to thank the United States Department of Agriculture National Agroforestry Center for their support. Additional thanks goes to Fred Garst of the Natural Resources Conservation Service, Beth Stein, Elizabeth Moore, Lukas Burgher, Yujuan Chen, and finally, the survey participants. NR 41 TC 1 Z9 1 U1 8 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0167-4366 EI 1572-9680 J9 AGROFOREST SYST JI Agrofor. Syst. PD AUG PY 2014 VL 88 IS 4 SI SI BP 619 EP 629 DI 10.1007/s10457-014-9678-5 PG 11 WC Agronomy; Forestry SC Agriculture; Forestry GA AM7LW UT WOS:000340049800006 ER PT J AU Shin, JH Xu, L Li, RW Gao, Y Bickhart, D Liu, GE Baldwin, R Li, CJ AF Shin, J. H. Xu, L. Li, R. W. Gao, Y. Bickhart, D. Liu, G. E. Baldwin, R. Li, C-J TI A high-resolution whole-genome map of the distinctive epigenomic landscape induced by butyrate in bovine cells SO ANIMAL GENETICS LA English DT Article DE butyrate; ChIP-seq; epigenetics; epigenomics; gene; histone acetylation; histone modification; regulation ID KIDNEY EPITHELIAL-CELLS; C-MYC; HISTONE ACETYLATION; GENE-EXPRESSION; BINDING-SITE; LYSINE 9; CHROMATIN; TRANSCRIPTION; PROMOTER; DEACETYLATION AB This report presents a study utilizing next-generation sequencing technology, combined with chromatin immunoprecipitation (ChIP-seq) technology to analyze histone modification induced by butyrate and to construct a high-definition map of the epigenomic landscape with normal histone H3 and H4 and their variants in bovine cells at the whole-genome scale. A total of 10 variants of histone H3 and H4 modifications were mapped at the whole-genome scale (acetyl-H3K18-ChIP-seq, trimethy-H3K9, histone H4 ChIP-seq, acetyl-H4K5 ChIP-seq, acetyl-H4K12 ChIP-seq, acetyl-H4K16 ChIP-seq, histone H3 ChIP-seq, acetyl H3H9 ChIP-seq, acetyl H3K27 ChIP-seq and tetra-acetyl H4 ChIP-seq). Integrated experiential data and an analysis of histone and histone modification at a single base resolution across the entire genome are presented. We analyzed the enriched binding regions in the proximal promoter (within 5 kb upstream or at the 5'-untranslated region from the transcriptional start site (TSS)), and the exon, intron and intergenic regions (defined by regions 25 kb upstream and 10 kb downstream from the TSS). A de novo search for the binding motif of the 10 ChIP-seq datasets discovered numerous motifs from each of the ChIP-seq datasets. These consensus sequences indicated that histone modification at different locations changes the histone H3 and H4 binding preferences. Nevertheless, a high degree of conservation in histone binding also was presented in these motifs. This first extensive epigenomic landscape mapping in bovine cells offers a new framework and a great resource for testing the role of epigenomes in cell function and transcriptomic regulation. C1 [Shin, J. H.; Gao, Y.] Johns Hopkins Univ, Lieber Inst Brain Dev, Baltimore, MD 21205 USA. [Xu, L.; Li, R. W.; Bickhart, D.; Liu, G. E.; Baldwin, R.; Li, C-J] ARS, Anim Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. RP Li, CJ (reprint author), 10300 Baltimore Ave,Bldg 306,Room 216, Beltsville, MD 20705 USA. EM congjun.li@ars.usda.gov OI Bickhart, Derek/0000-0003-2223-9285 NR 43 TC 0 Z9 0 U1 0 U2 12 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0268-9146 EI 1365-2052 J9 ANIM GENET JI Anim. Genet. PD AUG PY 2014 VL 45 SU 1 SI SI BP 40 EP 50 DI 10.1111/age.12147 PG 11 WC Agriculture, Dairy & Animal Science; Genetics & Heredity SC Agriculture; Genetics & Heredity GA AN0BD UT WOS:000340245500005 PM 24990294 ER PT J AU Brandl, MT Huynh, S AF Brandl, Maria T. Huynh, Steven TI Effect of the Surfactant Tween 80 on the Detachment and Dispersal of Salmonella enterica Serovar Thompson Single Cells and Aggregates from Cilantro Leaves as Revealed by Image Analysis SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ESCHERICHIA-COLI O157-H7; BIOFILM FORMATION; LEAF SURFACES; FRESH PRODUCE; LETTUCE; DECONTAMINATION; BACTERIA; QUANTIFICATION; HYDROPHOBICITY; PHYLLOSPHERE AB Salmonella enterica has the ability to form biofilms and large aggregates on produce surfaces, including on cilantro leaves. Aggregates of S. enterica serovar Thompson that remained attached to cilantro leaves after rigorous washing and that were present free or bound to dislodged leaf tissue in the wash suspension were observed by confocal microscopy. Measurement of S. Thompson population sizes in the leaf washes by plate counts failed to show an effect of 0.05% Tween 80 on the removal of the pathogen from cilantro leaves 2 and 6 days after inoculation. On the contrary, digital image analysis of micrographs of single cells and aggregates of green fluorescent protein (GFP)-S. Thompson present in cilantro leaf washes revealed that single cells represented 13.7% of the cell assemblages in leaf washes containing Tween 80, versus 9.3% in those without the surfactant. Moreover, Tween 80 decreased the percentage of the total S. Thompson cell population located in aggregates equal to or larger than 64 cells from 9.8% to 4.4% (P < 0.05). Regression analysis of the frequency distribution of aggregate size in leaf washes with and without Tween 80 showed that the surfactant promoted the dispersal of cells from large aggregates into smaller ones and into single cells (P < 0.05). Our study underlines the importance of investigating bacterial behavior at the scale of single cells in order to uncover trends undetectable at the population level by bacterial plate counts. Such an approach may provide valuable information to devise strategies aimed at enhancing the efficacy of produce sanitization treatments. C1 [Brandl, Maria T.; Huynh, Steven] ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. RP Brandl, MT (reprint author), ARS, Produce Safety & Microbiol Res Unit, USDA, Albany, CA 94710 USA. EM maria.brandl@ars.usda.gov FU U.S. Department of Agriculture, Agricultural Research Service CRIS [5325-42000-046] FX This research was supported by the U.S. Department of Agriculture, Agricultural Research Service CRIS project 5325-42000-046. NR 27 TC 4 Z9 4 U1 3 U2 28 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD AUG PY 2014 VL 80 IS 16 BP 5037 EP 5042 DI 10.1128/AEM.00795-14 PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AM7IC UT WOS:000340038400028 PM 24907336 ER PT J AU Rocaboy-Faquet, E Noguer, T Romdhane, S Bertrand, C Dayan, FE Barthelmebs, L AF Rocaboy-Faquet, Emilie Noguer, Thierry Romdhane, Sana Bertrand, Cedric Dayan, Franck Emmanuel Barthelmebs, Lise TI Novel bacterial bioassay for a high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Bioassay; HPPD; beta-Triketones; Sol-gel; E. coli ID P-HYDROXYPHENYLPYRUVATE DIOXYGENASE; ESCHERICHIA-COLI; LEPTOSPERMUM-SCOPARIUM; LIQUID-CHROMATOGRAPHY; CRYSTAL-STRUCTURES; MESOTRIONE; SULCOTRIONE; SOIL; ACETYLCHOLINESTERASE; METABOLITES AB Plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of synthetic beta-triketone herbicides that are currently used commercially. Their mode of action is based on an irreversible inhibition of HPPD. Therefore, this inhibitory capacity was used to develop a whole-cell colorimetric bioassay with a recombinant Escherichia coli expressing a plant HPPD for the herbicide analysis of beta-triketones. The principle of the bioassay is based on the ability of the recombinant E. coli clone to produce a soluble melanin-like pigment, from tyrosine catabolism through p-hydroxyphenylpyruvate and homogentisate. The addition of sulcotrione, a HPPD inhibitor, decreased the pigment production. With the aim to optimize the assay, the E. coli recombinant clone was immobilized in sol-gel or agarose matrix in a 96-well microplate format. The limit of detection for mesotrione, tembotrione, sulcotrione, and leptospermone was 0.069, 0.051, 0.038, and 20 mu M, respectively, allowing to validate the whole-cell colorimetric bioassay as a simple and cost-effective alternative tool for laboratory use. The bioassay results from sulcotrione-spiked soil samples were confirmed with high-performance liquid chromatography. C1 [Rocaboy-Faquet, Emilie; Noguer, Thierry; Romdhane, Sana; Barthelmebs, Lise] Univ Perpignan, Inst Modelisat & Anal Geoenvironm & Sante, F-66860 Perpignan, France. [Romdhane, Sana; Bertrand, Cedric] Univ Perpignan, Lab Chim Biomol & Environm, F-66860 Perpignan, France. [Dayan, Franck Emmanuel] Univ Mississippi, Nat Prod Utilizat Res Unit, USDA, ARS, Oxford, MS 38677 USA. RP Barthelmebs, L (reprint author), Univ Perpignan, Inst Modelisat & Anal Geoenvironm & Sante, Via Domitia,EA 4218, F-66860 Perpignan, France. EM barthelm@univ-perp.fr RI Dayan, Franck/A-7592-2009 OI Dayan, Franck/0000-0001-6964-2499 FU UPVD; research project TRICETOX [ANR-13-CESA-0002] FX Emilie Rocaboy is very grateful to UPVD for financial support. This study was carried out as the part of the research project TRICETOX through the ANR-13-CESA-0002. NR 49 TC 4 Z9 4 U1 5 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD AUG PY 2014 VL 98 IS 16 BP 7243 EP 7252 DI 10.1007/s00253-014-5793-5 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AM7ZM UT WOS:000340087600031 PM 24816780 ER PT J AU Stewart, J Parsons, AJ Wainwright, J Okin, GS Bestelmeyer, BT Fredrickson, EL Schlesinger, WH AF Stewart, J. Parsons, A. J. Wainwright, J. Okin, G. S. Bestelmeyer, B. T. Fredrickson, E. L. Schlesinger, W. H. TI Modeling emergent patterns of dynamic desert ecosystems SO ECOLOGICAL MONOGRAPHS LA English DT Article DE patchiness; positive feedback; self-organization; semiarid landscape ecology; spatially explicit model; vegetation patterns ID SOUTHERN NEW-MEXICO; TRANSPORT-DISTANCE APPROACH; SCALING EROSION RATES; SEMIDESERT GRASSLAND RANGE; SEMIARID GRAZING SYSTEMS; INTERRILL OVERLAND-FLOW; CHIHUAHUAN DESERT; ARID ECOSYSTEMS; LARREA-TRIDENTATA; VEGETATION CHANGE AB In many desert ecosystems, vegetation is both patchy and dynamic: vegetated areas are interspersed with patches of bare ground, and both the positioning and the species composition of the vegetated areas exhibit change through time. These characteristics lead to the emergence of multi-scale patterns in vegetation that arise from complex relationships between plants, soils, and transport processes. Previous attempts to probe the causes of spatial complexity and predict responses of desert ecosystems tend to be limited in their focus: models of dynamics have been developed with no consideration of the inherent patchiness in the vegetation, or else models have been developed to generate patterns with no consideration of the dynamics. Here we develop a general modelling framework for the analysis of ecosystem change in deserts that is rooted in the concept of connectivity and is derived from a detailed process-based understanding. We explicitly consider spatial interactions among multiple vegetation types and multiple resources, and our model is formulated to predict responses to a variety of endogenous and exogenous disturbances. The model is implemented in the deserts of the American Southwest both to test hypotheses of the causes of the invasion of woody shrubs, and to test its ability to reproduce observed spatial differences in response to drought in the 20th century. The model's performance leads us to argue that vertical and lateral connectivity are key emergent properties of the ecosystem, which both control its behavior and provide indicators of its state. If this argument is shown to be compatible with field observations, the model presented here will provide a more certain approach toward preventing further degradation of semiarid grasslands. C1 [Stewart, J.] Lincoln Univ, Sch Engn, Lincoln LN6 7TS, England. [Parsons, A. J.] Univ Sheffield, Dept Geog, Sheffield Ctr Int Drylands Res, Sheffield S10 2TN, S Yorkshire, England. [Wainwright, J.] Univ Durham, Sci Labs, Dept Geog, Durham DH1 3LE, England. [Okin, G. S.] Univ Calif Los Angeles, Dept Geog, Los Angeles, CA 90095 USA. [Bestelmeyer, B. T.] USDA ARS, Las Cruces, NM 88001 USA. [Fredrickson, E. L.] Eastern Kentucky Univ, Dept Agr, Richmond, KY 40475 USA. [Schlesinger, W. H.] Cary Inst Ecosyst Studies, Millbrook, NY 12545 USA. RP Parsons, AJ (reprint author), Univ Sheffield, Dept Geog, Sheffield Ctr Int Drylands Res, Sheffield S10 2TN, S Yorkshire, England. EM a.j.parsons@sheffield.ac.uk OI parsons, anthony/0000-0002-6730-5031 FU University of Sheffield; LTER [DEB-0618210] FX This research was supported by the University of Sheffield and LTER Grant DEB-0618210. NR 169 TC 13 Z9 13 U1 9 U2 59 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9615 EI 1557-7015 J9 ECOL MONOGR JI Ecol. Monogr. PD AUG PY 2014 VL 84 IS 3 BP 373 EP 410 DI 10.1890/12-1253.1 PG 38 WC Ecology SC Environmental Sciences & Ecology GA AM7XL UT WOS:000340081900002 ER PT J AU Resasco, J Haddad, NM Orrock, JL Shoemaker, D Brudvig, TA Damschen, EI Tewksbury, JJ Levey, DJ AF Resasco, Julian Haddad, Nick M. Orrock, John L. Shoemaker, DeWayne Brudvig, Tars A. Damschen, Ellen I. Tewksbury, Joshua J. Levey, Douglas J. TI Landscape corridors can increase invasion by an exotic species and reduce diversity of native species SO ECOLOGY LA English DT Article DE ants; biodiversity; biological invasion; connectivity; corridor experiment; fire ant; habitat fragmentation; invasive ants; monogyne vs. polygyne ants; Savannah River Site, South Carolina, USA; Solenopsis invicta; species richness ID IMPORTED FIRE ANT; SOLENOPSIS-INVICTA; FRAGMENTED LANDSCAPES; PLANT-COMMUNITIES; ARTHROPOD-COMMUNITY; CONNECTIVITY; DISPERSAL; POLYGYNE; RICHNESS; PATTERNS AB Landscape corridors are commonly used to mitigate negative effects of habitat fragmentation, but concerns persist that they may facilitate the spread of invasive species. In a replicated landscape experiment of open habitat, we measured effects of corridors on the invasive fire ant, Solenopsis invicta, and native ants. Fire ants have two social forms: polygyne, which tend to disperse poorly but establish at high densities, and monogyne, which disperse widely but establish at lower densities. In landscapes dominated by polygyne fire ants, fire ant abundance was higher and native ant diversity was lower in habitat patches connected by corridors than in unconnected patches. Conversely, in landscapes dominated by monogyne fire ants, connectivity had no influence on fire ant abundance and native ant diversity. Polygyne fire ants dominated recently created landscapes, suggesting that these corridor effects may be transient. Our results suggest that corridors can facilitate invasion and they highlight the importance of considering species' traits when assessing corridor utility. C1 [Resasco, Julian; Levey, Douglas J.] Univ Florida, Dept Biol, Gainesville, FL 32611 USA. [Haddad, Nick M.] N Carolina State Univ, Dept Biol Sci, Raleigh, NC 27695 USA. [Orrock, John L.; Damschen, Ellen I.] Univ Wisconsin, Dept Zool, Madison, WI 53706 USA. [Shoemaker, DeWayne] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Brudvig, Tars A.] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Tewksbury, Joshua J.] Univ Washington, Dept Biol, Seattle, WA 98195 USA. RP Resasco, J (reprint author), Univ Colorado, Dept Ecol & Evolutionary Biol, UCB 334, Boulder, CO 80309 USA. EM jresasco@colorado.edu FU NSF [DEB-0614333]; NSF (NSF-GRFP) [DGE-0802270]; DOE, Aiken, South Carolina [DE-AI09-00SR22188]; USDA NIFA [2009-35302-05301] FX We thank the Corridor Research Group for continued dialogue and insight on corridor function. We thank the USDA Forest Service-Savannah River for creating and maintaining our experimental landscapes; we are especially grateful to J. Blake, E. Olson, C. Hobson, and J. Segar. We thank L. Sullivan for field assistance, S. Porter, R. Fletcher, T. Palmer, and S. Robinson for useful comments, H. Penn and E. Carroll for help in the lab, L. Davis and M. Deyrup for assistance with ant identification, M. Resasco-Pratt for illustrations, and the USDA-ARS Gainesville, Florida. This research was supported by NSF (DEB-0614333 and an NSF-GRFP under grant DGE-0802270), by funds provided to the USDA Forest Service-Savannah River, under Interagency Agreement DE-AI09-00SR22188 with DOE, Aiken, South Carolina, and by the USDA NIFA (grant number 2009-35302-05301). NR 54 TC 12 Z9 12 U1 6 U2 194 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD AUG PY 2014 VL 95 IS 8 BP 2033 EP 2039 PG 7 WC Ecology SC Environmental Sciences & Ecology GA AM9QK UT WOS:000340215900001 PM 25230454 ER PT J AU Bell, DM Bradford, JB Lauenroth, WK AF Bell, David M. Bradford, John B. Lauenroth, William K. TI Forest stand structure, productivity, and age mediate climatic effects on aspen decline SO ECOLOGY LA English DT Article DE climate effects; drought; Forest Inventory and Analysis; forest stand structure; mortality; Populus tremuloides; quaking aspen; Rocky Mountain West; sudden aspen decline; United States ID POPULUS-TREMULOIDES; QUAKING ASPEN; BIOMASS ALLOCATION; PONDEROSA PINE; DIE-OFF; MORTALITY; DROUGHT; LANDSCAPE; COLORADO; CARBON AB Because forest stand structure, age, and productivity can mediate the impacts of climate on quaking aspen (Populus tremuloides) mortality, ignoring stand-scale factors limits. inference on the drivers of recent sudden aspen decline. Using the proportion of aspen trees that were dead as an index of recent mortality at 841 forest inventory plots, we examined the relationship of this mortality index to forest structure and climate in the Rocky Mountains and Intermountain Western United States. We found that forest structure explained most of the patterns in mortality indices, but that variation in growing-season vapor pressure deficit and winter precipitation over the last 20 years was important. Mortality index sensitivity to precipitation was highest in forests where aspen exhibited high densities, relative basal areas, quadratic mean diameters, and productivities, whereas sensitivity to vapor pressure deficit was highest in young forest stands. These results indicate that the effects of drought on mortality may be mediated by forest stand development, competition with encroaching conifers, and physiological vulnerabilities of large trees to drought. By examining mortality index responses to both forest structure and climate, we show that forest succession cannot be ignored in studies attempting to understand the causes and consequences of sudden aspen decline. C1 [Bell, David M.; Lauenroth, William K.] Univ Wyoming, Dept Bot, Laramie, WY 82072 USA. [Bradford, John B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86011 USA. RP Bell, DM (reprint author), US Forest Serv, Pacific NW Res Stn, Forestry Sci Lab, 3200 SW Jefferson Way, Corvallis, OR 97331 USA. EM dmbell@fs.fed.us RI Bradford, John/E-5545-2011; OI Bell, David/0000-0002-2673-5836 FU University of Wyoming; U.S. Geological Survey, Southwest Biological Science Center [G11AC20366]; NSF Postdoctoral Research Fellowship in Biology [1202800] FX Funding for this work was provided by the University of Wyoming and the U.S. Geological Survey, Southwest Biological Science Center via cooperative agreement #G11AC20366 and the NSF Postdoctoral Research Fellowship in Biology #1202800. The manuscript was improved by comments from three anonymous reviewers. Any use of trade, product, or firm names is for descriptive purposes only and does not imply endorsement by the U.S. Government. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of NSF. NR 36 TC 5 Z9 5 U1 3 U2 67 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD AUG PY 2014 VL 95 IS 8 BP 2040 EP 2046 PG 7 WC Ecology SC Environmental Sciences & Ecology GA AM9QK UT WOS:000340215900002 PM 25230455 ER PT J AU Moran, MS Ponce-Campos, GE Huete, A McClaran, MP Zhang, YG Hamerlynck, EP Augustine, DJ Gunter, SA Kitchen, SG Peters, DPC Starks, PJ Hernandez, M AF Moran, M. Susan Ponce-Campos, Guillermo E. Huete, Alfredo McClaran, Mitchel P. Zhang, Yongguang Hamerlynck, Erik P. Augustine, David J. Gunter, Stacey A. Kitchen, Stanley G. Peters, Debra P. C. Starks, Patrick J. Hernandez, Mariano TI Functional response of U.S. grasslands to the early 21st-century drought SO ECOLOGY LA English DT Article DE climate change; desert; extreme events; grassland production; invasive species; plains; precipitation variability; resilience; warm drought ID NET PRIMARY PRODUCTION; CLIMATE-CHANGE; UNITED-STATES; GLOBAL-CHANGE; TALLGRASS PRAIRIE; ECOSYSTEM; VEGETATION; BIOMES; NORTH; DYNAMICS AB Grasslands across the United States play a key role in regional livelihood and national food security. Yet, it is still unclear how this important resource will respond to the prolonged warm droughts and more intense rainfall events predicted with climate change. The early 21st-century drought in the southwestern United States resulted in hydroclimatic conditions that are similar to those expected with future climate change. We investigated the impact of the early 21st-century drought on aboveground net primary production (ANPP) of six desert and plains grasslands dominated by C-4 (warm season) grasses in terms of significant deviations between observed and expected ANPP. In desert grasslands, drought-induced grass mortality led to shifts in the functional response to annual total precipitation (P-T), and in some cases, new species assemblages occurred that included invasive species. In contrast, the ANPP in plains grasslands exhibited a strong linear function of the current-year P-T and the previous-year ANPP, despite prolonged warm drought. We used these results to disentangle the impacts of interannual total precipitation, intra-annual precipitation patterns, and grassland abundance on ANPP, and thus generalize the functional response of C-4 grasslands to predicted climate change. This will allow managers to plan for predictable shifts in resources associated with climate change related to fire risk, loss of forage, and ecosystem services. C1 [Moran, M. Susan; Ponce-Campos, Guillermo E.; Zhang, Yongguang; Hamerlynck, Erik P.; Hernandez, Mariano] USDA ARS, Southwest Watershed Res, Tucson, AZ 85719 USA. [Huete, Alfredo] Univ Technol Sydney, Plant Funct Biol & Climate Change Cluster, Sydney, NSW 2007, Australia. [McClaran, Mitchel P.] Univ Arizona, Sch Nat Resources & Environm, Tucson, AZ 85721 USA. [Augustine, David J.] USDA ARS, Rangeland Resources Res Unit, Ft Collins, CO 80526 USA. [Gunter, Stacey A.] USDA ARS, Southern Plains Range Res Stn, Woodward, OK 73801 USA. [Kitchen, Stanley G.] US Forest Serv, USDA, Rocky Mt Res Stn, Provo, UT 84606 USA. [Peters, Debra P. C.] USDA ARS, Jornada Expt Range, Las Cruces, NM 30003 USA. [Starks, Patrick J.] USDA ARS, Grazing Lands Res Lab, El Reno, OK 73036 USA. RP Moran, MS (reprint author), USDA ARS, Southwest Watershed Res, Tucson, AZ 85719 USA. EM susan.moran@ars.usda.gov RI Huete, Alfredo/C-1294-2008; OI Huete, Alfredo/0000-0003-2809-2376; Zhang, Yongguang/0000-0001-8286-300X FU NASA [08-SMAPSDT08-0042]; NSF [DEB-1235828]; USDA CSREES grant [2008-51130-19567] FX This study has been supported in part by NASA (08-SMAPSDT08-0042), NSF (DEB-1235828), and USDA CSREES grant 2008-51130-19567. We also thank the Eco-Trends Project for access to multisite, long-term data and the generous contributors to ANPP measurements, including J. Derner (CPL) and J. Anderson (JRN). NR 60 TC 21 Z9 21 U1 11 U2 100 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 0012-9658 EI 1939-9170 J9 ECOLOGY JI Ecology PD AUG PY 2014 VL 95 IS 8 BP 2121 EP 2133 PG 13 WC Ecology SC Environmental Sciences & Ecology GA AM9QK UT WOS:000340215900011 PM 25230464 ER PT J AU Engle-Stone, R Haskell, MJ La Frano, MR Ndjebayi, AO Nankap, M Brown, KH AF Engle-Stone, R. Haskell, M. J. La Frano, M. R. Ndjebayi, A. O. Nankap, M. Brown, K. H. TI Comparison of breast milk vitamin A concentration measured in fresh milk by a rapid field assay (the iCheck FLUORO) with standard measurement of stored milk by HPLC SO EUROPEAN JOURNAL OF CLINICAL NUTRITION LA English DT Article ID WOMEN; CHILDREN; RETINOL; PLASMA AB Availability of rapid, point-of-contact analytical methods would facilitate the use of breast milk vitamin A concentration (BMVA) to assess vitamin A (VA) status. We compared BMVA concentrations measured by high-performance liquid chromatography (HPLC) (the standard technique) with those by iCheck, FLUORO, a new portable fluorometer that can rapidly quantify BMVA. Casual breast milk samples (n=154) were collected during a representative survey in Yaounde and Douala, Cameroon. Milk fat and BMVA concentrations (by iCheck) were measured in fresh milk in the field. After storage at < 20 degrees C, BMVA concentrations were also measured by HPLC. BMVA values from the two methods were highly correlated (R-2=0.72 for BMVA/I; R-2= 0.62 for BMVA/g fat, both P <0.0001). HPLC values were greater than iCheck values on average, and the difference increased with increasing BMVA. The iCheck FLUORO could be useful for monitoring fortification programs, but before-after surveys to assess change in BMVA concentrations should use one method consistently. C1 [Engle-Stone, R.; Haskell, M. J.; Brown, K. H.] Univ Calif Davis, Program Int & Community Nutr, Dept Nutr, Davis, CA 95616 USA. [La Frano, M. R.] USDA Western Human Nutr Res Ctr, Davis, CA USA. [Ndjebayi, A. O.; Nankap, M.] Helen Keller Int, Yaounde, Cameroon. RP Engle-Stone, R (reprint author), Univ Calif Davis, Program Int & Community Nutr, Dept Nutr, One Shields Ave, Davis, CA 95616 USA. EM renglestone@ucdavis.edu FU Sight and Life (Basel, Switzerland) FX We thank Daniela Hampel (USDA, Davis, CA, USA) for NIST formula verification. This study was supported by Sight and Life (Basel, Switzerland). NR 9 TC 1 Z9 1 U1 1 U2 14 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0954-3007 EI 1476-5640 J9 EUR J CLIN NUTR JI Eur. J. Clin. Nutr. PD AUG PY 2014 VL 68 IS 8 BP 938 EP 940 DI 10.1038/ejcn.2014.63 PG 3 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AN0XV UT WOS:000340308200013 PM 24736678 ER PT J AU Page, WG Jenkins, MJ Runyon, JB AF Page, Wesley G. Jenkins, Michael J. Runyon, Justin B. TI Spruce Beetle-Induced Changes to Engelmann Spruce Foliage Flammabiliiy SO FOREST SCIENCE LA English DT Article DE crown fire; bark beetles; heat of combustion; fire behavior; time to ignition ID WILDLAND-URBAN INTERFACE; WESTERN NORTH-AMERICA; SUB-ALPINE FORESTS; BARK BEETLES; REGRESSION-MODELS; MOISTURE CONTENTS; ROCKY-MOUNTAINS; TREE MORTALITY; CROWN FIRE; WILDFIRE AB Intermountain Engelmann spruce (Picea engelmannii Parry ex Engelm) stands affected by the spruce beetle (Dendroctonus rufipennis Kirby) represent a unique and growing fuel complex. In this study, we quantified and compared the changes in moisture content, chemistry, and flammability of foliage from trees in three crown condition classes: unattacked (green [G]), currently mass attacked (green-infested [Cl]), and mass attacked the previous year (yellow (1]) over the course of a fire season. Cl trees displayed highly variable decreases in moisture content both between trees and within individual tree crowns that produced variable increases in flammability. The foliage on Y trees had significantly lower moisture contents, higher proportions of lignin and cellulose, and lower proportions of carbohydrate-based compounds than G foliage, which resulted in increased flammability. This increase in crown flammability was short-lived because the foliage on Y trees dropped abruptly approximately 14 months after mass attack (by late July). Given the observed changes in flammability, increased crown fire potential may occur in spruce beetle-infested forests during the spring when G and Y foliage flammability is highest, provided sufficiently dry conditions, and in late summer when the combination of peak GI foliage flammability coincides with the peak in seasonal drying. C1 [Page, Wesley G.; Jenkins, Michael J.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Runyon, Justin B.] USDA, Forest Serv, Rocky Mt Res Stn, Bozeman, MT USA. RP Page, WG (reprint author), Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. EM wgp6@hotmail.com; mike.jenkins@usu.edu; jrunyon@fs.fed.us FU Joint Fire Science Program [11-1-4-16] FX Funding for this study was provided by Joint Fire Science Program Project 11-1-4-16. We thank J. Robinson for help with fieldwork, L. Teng at the Utah State University BioEnergy Center for laboratory equipment support, and W.E. Lindquist for assistance with graphics. The comments of M. E. Alexander, B. J. Bentz, E. W Evans, E. G. Hebertson, M. R. Kuhns, three anonyms reviewers, and the associate editor are gratefully acknowledged. The help of Jim Gibson from the Uinta-Wasatch-Cache National Forest for field site approval is also acknowledged. NR 72 TC 4 Z9 4 U1 4 U2 13 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD AUG PY 2014 VL 60 IS 4 BP 691 EP 702 DI 10.5849/forsci.13-050 PG 12 WC Forestry SC Forestry GA AM9PD UT WOS:000340212600009 ER PT J AU Fairweather, ML Rokala, EA Mock, KE AF Fairweather, Mary L. Rokala, E. A. Mock, Karen E. TI Aspen Seedling Establishment and Growth after Wildfire in Central Arizona: An Instructive Case History SO FOREST SCIENCE LA English DT Article DE aspen; seedlings; ungulate; fire; exclosure ID YELLOWSTONE-NATIONAL-PARK; HIGH-ELEVATION FOREST; POPULUS-TREMULOIDES; NORTHERN ARIZONA; GENETIC DIVERSITY; AMERICAN ASPENS; PONDEROSA PINE; USA; FIRE; ELK AB Reproduction of aspen (Populus tremuloides Michx) occurs primarily through asexual root suckering, and establishment by seed in western US landscapes has been considered extremely rare. Here, we describe a natural aspen seedling recruitment event after wildfire in Arizona, occurring during severe drought within low elevation, fenced ponderosa pine plantations. Aspen was not present in these prefire sites, and genetic analysis confirmed that 70 individuals in these exclosures were unique. Eleven years postfire, average seedling diameter was 4.2 cm and average height was 2.3 m. Seedlings established over a 6-year period, with 91% establishing in the first 3 years. Clonal development was both diverse and robust: 61% of seedlings produced 1-39 suckers, and the total number of suckers (n = 246) now far exceeds the 70 seedlings. Aspen seedlings established during severe drought years had a greater association with large woody debris than those established later. The establishment of aspen seedlings during severe drought, along with the relative abundance of aspen seedlings in fenced exclosures (with only two seedlings observed outside exclosures) suggests that aspen seedling establishment in at least some locations may be more limited by herbivory than directly by warming climate. C1 [Fairweather, Mary L.] USDA, Forest Serv, Forest Hlth Protect, Flagstaff, AZ 86001 USA. [Rokala, E. A.] USDA, Forest Serv, Kaibab Natl Forest, Flagstaff, AZ 86001 USA. [Mock, Karen E.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Mock, Karen E.] Utah State Univ, Quinney Coll Nat Resources, Logan, UT 84322 USA. RP Fairweather, ML (reprint author), USDA, Forest Serv, Forest Hlth Protect, Flagstaff, AZ 86001 USA. EM mfairweather@fs.fed.us; erokala@fs.fed.us; karen.mock@usu.edu RI Mock , Karen/C-1418-2011 FU USDA Forest Service, Southwestern Region, Arizona Zone Forest Health Protection FX We thank Jason Stevens, Kaibab National Forest, and Mandy Roesch, Coconino National Forest, for providing grazing information. Carol Rowe provided molecular analyses. Dan Ryerson, Southwestern Regional Office, USDA Forest Service, provided GIS assistance. This work was supported by funding from the USDA Forest Service, Southwestern Region, Arizona Zone Forest Health Protection. We thank two anonymous reviewers for their helpful comments. NR 70 TC 7 Z9 7 U1 4 U2 38 PU SOC AMER FORESTERS PI BETHESDA PA 5400 GROSVENOR LANE, BETHESDA, MD 20814 USA SN 0015-749X EI 1938-3738 J9 FOREST SCI JI For. Sci. PD AUG PY 2014 VL 60 IS 4 BP 703 EP 712 DI 10.5849/forsci.13-048 PG 10 WC Forestry SC Forestry GA AM9PD UT WOS:000340212600010 ER PT J AU Kennedy, RE Andrefouet, S Cohen, WB Gomez, C Griffiths, P Hais, M Healey, SP Helmer, EH Hostert, P Lyons, MB Meigs, GW Pflugmacher, D Phinn, SR Powell, SL Scarth, P Sen, S Schroeder, TA Schneider, A Sonnenschein, R Vogelmann, JE Wulder, MA Zhu, Z AF Kennedy, Robert E. Andrefouet, Serge Cohen, Warren B. Gomez, Cristina Griffiths, Patrick Hais, Martin Healey, Sean P. Helmer, Eileen H. Hostert, Patrick Lyons, Mitchell B. Meigs, Garrett W. Pflugmacher, Dirk Phinn, Stuart R. Powell, Scott L. Scarth, Peter Sen, Susmita Schroeder, Todd A. Schneider, Annemarie Sonnenschein, Ruth Vogelmann, James E. Wulder, Michael A. Zhu, Zhe TI Bringing an ecological view of change to Landsat-based remote sensing SO FRONTIERS IN ECOLOGY AND THE ENVIRONMENT LA English DT Review ID TIME-SERIES; FOREST DISTURBANCE; COVER CHANGE; TREND ANALYSIS; ECOSYSTEM; IMAGERY; SURFACE; AREA; SATELLITE; HABITAT AB When characterizing the processes that shape ecosystems, ecologists increasingly use the unique perspective offered by repeat observations of remotely sensed imagery. However, the concept of change embodied in much of the traditional remote-sensing literature was primarily limited to capturing large or extreme changes occurring in natural systems, omitting many more subtle processes of interest to ecologists. Recent technical advances have led to a fundamental shift toward an ecological view of change. Although this conceptual shift began with coarser-scale global imagery, it has now reached users of Landsat imagery, since these datasets have temporal and spatial characteristics appropriate to many ecological questions. We argue that this ecologically relevant perspective of change allows the novel characterization of important dynamic processes, including disturbances, long-term trends, cyclical functions, and feedbacks, and that these improvements are already facilitating our understanding of critical driving forces, such as climate change, ecological interactions, and economic pressures. C1 [Kennedy, Robert E.; Zhu, Zhe] Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. [Andrefouet, Serge] Inst Rech Dev, Noumea, New Caledonia. [Cohen, Warren B.] USDA Forest Serv, Pacific Northwest Res Stn, Corvallis, OR USA. [Gomez, Cristina] Univ Aberdeen, Sch Geosci, Aberdeen, Scotland. [Griffiths, Patrick; Hostert, Patrick; Pflugmacher, Dirk] Humboldt Univ, Dept Geog, D-10099 Berlin, Germany. [Hais, Martin] Univ South Bohemia, Fac Sci, Dept Ecosyst Biol, Ceske Budejovice, Czech Republic. [Healey, Sean P.; Schroeder, Todd A.] USDA Forest Serv, Rocky Mt Res Stn, Ogden, UT USA. [Helmer, Eileen H.] USDA Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Lyons, Mitchell B.] Univ New S Wales, Ctr Ecosyst Sci, Sch Biol Earth & Environm Sci, Kensington, NSW 2033, Australia. [Lyons, Mitchell B.; Phinn, Stuart R.; Scarth, Peter] Univ Queensland, Joint Remote Sensing Res Program, Sch Geog Planning & Environm Management, Brisbane, Qld, Australia. [Meigs, Garrett W.] Oregon State Univ, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Powell, Scott L.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Sen, Susmita] Virginia Tech Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA USA. [Schneider, Annemarie] Univ Wisconsin, Ctr Sustainabil & Global Environm, Madison, WI USA. [Sonnenschein, Ruth] Inst Appl Remote Sensing, EURAC, Bozen Bolzano, Italy. [Vogelmann, James E.] USGS EROS Data Ctr, Sioux Falls, SD USA. [Wulder, Michael A.] Nat Resources Canada, Pacif Forestry Ctr, Canadian Forest Serv, Victoria, BC, Canada. RP Kennedy, RE (reprint author), Boston Univ, Dept Earth & Environm, Boston, MA 02215 USA. EM kennedyr@bu.edu RI Griffiths, Patrick/O-4628-2014; Zhu, Zhe/K-3518-2015; Wulder, Michael/J-5597-2016; Gomez, Cristina/S-8139-2016; OI Griffiths, Patrick/0000-0002-8839-9022; Zhu, Zhe/0000-0001-8283-6407; Wulder, Michael/0000-0002-6942-1896; Gomez, Cristina/0000-0002-2756-0863; Vogelmann, James/0000-0002-0804-5823 NR 56 TC 45 Z9 48 U1 18 U2 85 PU ECOLOGICAL SOC AMER PI WASHINGTON PA 1990 M STREET NW, STE 700, WASHINGTON, DC 20036 USA SN 1540-9295 EI 1540-9309 J9 FRONT ECOL ENVIRON JI Front. Ecol. Environ. PD AUG PY 2014 VL 12 IS 6 BP 339 EP 346 DI 10.1890/130066 PG 8 WC Ecology; Environmental Sciences SC Environmental Sciences & Ecology GA AM7XY UT WOS:000340083200016 ER PT J AU Looft, T Allen, HK Cantarel, BL Levine, UY Bayles, DO Alt, DP Henrissat, B Stanton, TB AF Looft, Torey Allen, Heather K. Cantarel, Brandi L. Levine, Uri Y. Bayles, Darrell O. Alt, David P. Henrissat, Bernard Stanton, Thaddeus B. TI Bacteria, phages and pigs: the effects of in-feed antibiotics on the microbiome at different gut locations SO ISME JOURNAL LA English DT Article DE antibiotics; CAZy; gut ecology; metagenomics; microbiota; swine ID 16S RIBOSOMAL DNA; GASTROINTESTINAL-TRACT; INTESTINAL MICROBIOTA; PSEUDOMONAS-AERUGINOSA; MICROFLORA; MUCOSA; COLON; AMPLIFICATION; METAGENOMICS; POPULATIONS AB Disturbance of the beneficial gut microbial community is a potential collateral effect of antibiotics, which have many uses in animal agriculture (disease treatment or prevention and feed efficiency improvement). Understanding antibiotic effects on bacterial communities at different intestinal locations is essential to realize the full benefits and consequences of in-feed antibiotics. In this study, we defined the lumenal and mucosal bacterial communities from the small intestine (ileum) and large intestine (cecum and colon) plus feces, and characterized the effects of in-feed antibiotics (chlortetracycline, sulfamethazine and penicillin (ASP250)) on these communities. 16S rRNA gene sequence and metagenomic analyses of bacterial membership and functions revealed dramatic differences between small and large intestinal locations, including enrichment of Firmicutes and phage-encoding genes in the ileum. The large intestinal microbiota encoded numerous genes to degrade plant cell wall components, and these genes were lacking in the ileum. The mucosa-associated ileal microbiota harbored greater bacterial diversity than the lumen but similar membership to the mucosa of the large intestine, suggesting that most gut microbes can associate with the mucosa and might serve as an inoculum for the lumen. The collateral effects on the microbiota of antibiotic-fed animals caused divergence from that of control animals, with notable changes being increases in Escherichia coli populations in the ileum, Lachnobacterium spp. in all gut locations, and resistance genes to antibiotics not administered. Characterizing the differential metabolic capacities and response to perturbation at distinct intestinal locations will inform strategies to improve gut health and food safety. C1 [Looft, Torey; Allen, Heather K.; Levine, Uri Y.; Stanton, Thaddeus B.] USDA ARS, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, Ames, IA 50010 USA. [Cantarel, Brandi L.] Baylor Healthcare Syst, Baylor Inst Immunol Res, Dallas, TX USA. [Bayles, Darrell O.; Alt, David P.] ARS, Natl Anim Dis Ctr, Infectious Bacterial Dis Res Unit, Ames, IA USA. [Henrissat, Bernard] Aix Marseille Univ, CNRS, UMR 7257, Marseille, France. [Henrissat, Bernard] Univ Copenhagen, Fac Hlth & Med Sci, Dept Cellular & Mol Med, DK-2200 Copenhagen, Denmark. RP Allen, HK (reprint author), USDA ARS, Natl Anim Dis Ctr, Food Safety & Enter Pathogens Res Unit, 1920 Dayton Ave, Ames, IA 50010 USA. EM heather.allen@ars.usda.gov RI Henrissat, Bernard/J-2475-2012 NR 59 TC 42 Z9 43 U1 23 U2 138 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 1751-7362 EI 1751-7370 J9 ISME J JI ISME J. PD AUG PY 2014 VL 8 IS 8 BP 1566 EP 1576 DI 10.1038/ismej.2014.12 PG 11 WC Ecology; Microbiology SC Environmental Sciences & Ecology; Microbiology GA AM7FJ UT WOS:000340029800002 PM 24522263 ER PT J AU Hwang, CA Huang, LH AF Hwang, Cheng-An Huang, Lihan TI THE EFFECT OF POTASSIUM SORBATE AND pH ON THE GROWTH OF LISTERIA MONOCYTOGENES IN HAM SALAD SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID READY-TO-EAT; STORAGE-TEMPERATURE; UNITED-STATES; MAYONNAISE; PRODUCTS; PERFORMANCE; SALMONELLA; DRESSINGS; LEVEL; FOODS AB This study examined the growth of Listeria monocytogenes in ham salads of various pHs and sorbate concentrations. Ham was inoculated with L. monocytogenes and mixed with potassium sorbate (0-0.2%) and mayonnaise to achieve salad pHs of 5.4-5.8. The population increases of L. monocytogenes in salads stored at 4C for 4 weeks were correlated to salad pH and sorbate concentration. In salads with pHs of 5.4-5.8 containing 0.0, 0.1, 0.15 and 0.2% sorbate, the populations of L. monocytogenes increased 2.7-6.4, 2.4-5.2, 1.0-3.7 and 0.2-2.0 log cfu/g, respectively. A polynomial model was developed to describe the population increases as a function of salad pH and sorbate concentration. It indicated that the increases of L. monocytogenes were significantly lower (P < 0.05) in salads containing higher sorbate concentrations. Results from this study will help identify the salad pH and sorbate levels that reduce the hazard of L. monocytogenes in mayonnaise-based salads. C1 [Hwang, Cheng-An; Huang, Lihan] ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Hwang, CA (reprint author), ARS, Residue Chem & Predict Microbiol Res Unit, Eastern Reg Res Ctr, USDA, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM Andy.Hwang@ars.usda.gov NR 22 TC 1 Z9 1 U1 3 U2 14 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD AUG PY 2014 VL 38 IS 4 BP 1511 EP 1516 DI 10.1111/jfpp.12110 PG 6 WC Food Science & Technology SC Food Science & Technology GA AM8ZI UT WOS:000340168200013 ER PT J AU Onwulata, CI Tunick, MH Thomas-Gahring, AE AF Onwulata, Charles I. Tunick, Michael H. Thomas-Gahring, Audrey E. TI PASTING AND EXTRUSION PROPERTIES OF MIXED CARBOHYDRATE AND WHEY PROTEIN ISOLATE MATRICES SO JOURNAL OF FOOD PROCESSING AND PRESERVATION LA English DT Article ID TEXTURIZED DAIRY PROTEINS; FUNCTIONAL-PROPERTIES; PHYSICAL-PROPERTIES; EXTRUDED PRODUCTS; CASSAVA STARCH; CORN MEAL; FOOD; GELS; GELATION; SYSTEMS AB Mixed systems of whey protein isolate (WPI) or texturized WPI and different starches may form weak or strong gel pastes or rigid matrices depending on interactions. The paste viscoelasticity of starches from amioca, barley, cornstarch, Hylon VII, plantain and pea starch, mixed with whey protein isolate or textured WPI at the concentrations of 75/25, 50/50 or 25/75 wt% was determined using a Rapid Visco Analyzer. Peak paste ranged from plantain (5.33 Pa.s), amioca (4.14 Pa.s), cornstarch (3.65 Pa.s), barley (2.61 Pa.s), Hylon VII and pea starch (10 mPa.s). The presence of whey proteins reduced paste viscosity and extrusion expansion, but reduced hardness and improved fracturability. Moisture content was a stronger determinant of paste properties and of the denatured state of the whey protein isolate. C1 [Onwulata, Charles I.; Tunick, Michael H.; Thomas-Gahring, Audrey E.] ARS, Eastern Reg Res Ctr, USDA, Wyndmoor, PA 19038 USA. RP Onwulata, CI (reprint author), ARS, Eastern Reg Res Ctr, USDA, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Charles.Onwulata@ars.usda.gov NR 44 TC 2 Z9 2 U1 5 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0145-8892 EI 1745-4549 J9 J FOOD PROCESS PRES JI J. Food Process Preserv. PD AUG PY 2014 VL 38 IS 4 BP 1577 EP 1591 DI 10.1111/jfpp.12118 PG 15 WC Food Science & Technology SC Food Science & Technology GA AM8ZI UT WOS:000340168200020 ER PT J AU Poire, R Chochois, V Sirault, XRR Vogel, JP Watt, M Furbank, RT AF Poire, Richard Chochois, Vincent Sirault, Xavier R. R. Vogel, John P. Watt, Michelle Furbank, Robert T. TI Digital imaging approaches for phenotyping whole plant nitrogen and phosphorus response in Brachypodium distachyon SO JOURNAL OF INTEGRATIVE PLANT BIOLOGY LA English DT Article DE Nutrients uptake; phenomics; photosynthesis; root; shoot ID SPAD-502 CHLOROPHYLL METER; RELATIVE GROWTH-RATE; USE-EFFICIENCY; ROOT-GROWTH; CROP PLANTS; GENETIC-IMPROVEMENT; WHEAT GENOTYPES; NODAL ROOTS; PHOTOSYNTHESIS; RICE AB This work evaluates the phenotypic response of the model grass (Brachypodium distachyon (L.) P. Beauv.) to nitrogen and phosphorus nutrition using a combination of imaging techniques and destructive harvest of shoots and roots. Reference line Bd21-3 was grown in pots using 11 phosphorus and 11 nitrogen concentrations to establish a dose-response curve. Shoot biovolume and biomass, root length and biomass, and tissue phosphorus and nitrogen concentrations increased with nutrient concentration. Shoot biovolume, estimated by imaging, was highly correlated with dry weight (R-2 > 0.92) and both biovolume and growth rate responded strongly to nutrient availability. Higher nutrient supply increased nodal root length more than other root types. Photochemical efficiency was strongly reduced by low phosphorus concentrations as early as 1 week after germination, suggesting that this measurement may be suitable for high throughput screening of phosphorus response. In contrast, nitrogen concentration had little effect on photochemical efficiency. Changes in biovolume over time were used to compare growth rates of four accessions in response to nitrogen and phosphorus supply. We demonstrate that a time series image-based approach coupled with mathematical modeling provides higher resolution of genotypic response to nutrient supply than traditional destructive techniques and shows promise for high throughput screening and determination of genomic regions associated with superior nutrient use efficiency. C1 [Poire, Richard; Chochois, Vincent; Sirault, Xavier R. R.; Watt, Michelle; Furbank, Robert T.] CSIRO Plant Ind, Canberra, ACT 2601, Australia. [Poire, Richard; Sirault, Xavier R. R.; Furbank, Robert T.] CSIRO Plant Ind, High Resolut Plant Phenom Ctr, Canberra, ACT 2601, Australia. [Vogel, John P.] ARS, USDA, Western Reg Res Ctr, Albany, CA USA. RP Furbank, RT (reprint author), CSIRO Plant Ind, Canberra, ACT 2601, Australia. EM robert.furbank@csiro.au RI Watt, Michelle/F-8941-2010; Sirault, Xavier/B-3014-2008; Furbank, Robert/A-1884-2010; Watt, Michelle/I-6226-2016; OI Sirault, Xavier/0000-0002-4873-1844; Watt, Michelle/0000-0001-7843-0957; Vogel, John/0000-0003-1786-2689 FU Office of Science (BER), U.S. Department of Energy [DE-SC0001526]; Australian Grain Research and Development Corporation (GRDC) FX We acknowledge Manny Delhaize and Alan Richardson for useful and constructive discussions, Sol ne Callarec for her work on Experiment 2, and Sam Walker for excellent technical support. This research was supported by the Office of Science (BER), U.S. Department of Energy through Interagency Agreement DE-SC0001526 and the Australian Grain Research and Development Corporation (GRDC). NR 75 TC 7 Z9 7 U1 4 U2 56 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1672-9072 EI 1744-7909 J9 J INTEGR PLANT BIOL JI J. Integr. Plant Biol. PD AUG PY 2014 VL 56 IS 8 BP 781 EP 796 DI 10.1111/jipb.12198 PG 16 WC Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA AN0AV UT WOS:000340244400007 PM 24666962 ER PT J AU Mcroberts, RE Mech, LD AF Mcroberts, Ronald E. Mech, L. David TI Wolf Population Regulation Revisited-Again SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE biomass; Canis lupus; numerical response; population regulation; predation; prey biomass; wolf ID ISLE ROYALE WOLVES; EVOLUTIONARY HISTORY; CANIS-LUPUS; PERSPECTIVE; PREDATION AB The long-accepted conclusion that wolf density is regulated by nutrition was recently challenged, and the conclusion was reached that, at greater levels of prey biomass, social factors such as intraspecific strife and territoriality tend to regulate wolf density. We reanalyzed the data used in that study for 2 reasons: 1) we disputed the use of 2 data points, and 2) because of recognized heteroscedasticity, we used weighted-regression analysis instead of the unweighted regressions used in the original study. We concluded that the data do not support the hypothesis that wolf densities are regulated by social factors. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Mcroberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. [Mech, L. David] US Geol Survey, Northern Prairie Wildlife Res Ctr, Jamestown, ND 58401 USA. RP Mcroberts, RE (reprint author), US Forest Serv, No Res Stn, 1992 Folwell Ave, St Paul, MN 55108 USA. EM mechx002@umn.edu FU United States Geological Survey; United States Forest Service FX This study was supported by the United States Geological Survey and the United States Forest Service. We thank J. K. Oakleaf and 2 anonymous reviewers for reviewing the manuscript and offering helpful suggestions for improvement. NR 30 TC 4 Z9 4 U1 6 U2 77 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD AUG PY 2014 VL 78 IS 6 BP 963 EP 967 DI 10.1002/jwmg.744 PG 5 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AN0TN UT WOS:000340297000002 ER PT J AU Xu, XJ Feng, JC Lu, SY Lohrey, GT An, HL Zhou, YJ Jenks, MA AF Xu, Xiaojing Feng, Jinchao Lu, Shiyou Lohrey, Greg T. An, Huiling Zhou, Yijun Jenks, Matthew A. TI Leaf cuticular lipids on the Shandong and Yukon ecotypes of saltwater cress, Eutrema salsugineum, and their response to water deficiency and impact on cuticle permeability SO PHYSIOLOGIA PLANTARUM LA English DT Article ID ARABIDOPSIS ECERIFERUM1; ALKANE BIOSYNTHESIS; GENE-EXPRESSION; PEPPER CAPSICUM; SALT TOLERANCE; WAX; PLANTS; INVOLVEMENT; POLYESTERS; COMPONENTS AB The impact of water-deficit stress on leaf cuticular waxes and cutin monomers, and traits associated with cuticle permeability were examined in Shandong and Yukon ecotypes of Eutrema salsugineum (syn. Thellungiella salsuginea). Although Shandong exhibits glaucous leaves, and Yukon is non-glaucous, wax amounts on non-stressed Yukon leaves were 4.6-fold higher than on Shandong, due mainly to Yukon's eightfold higher wax fatty acids, especially the C-22 and C-24 acid homologues. Water deficit caused a 26.9% increase in total waxes on Shandong leaves, due mainly to increased C-22 and C-24 acids; and caused 10.2% more wax on Yukon, due mainly to an increase in wax alkanes. Total cutin monomers on non-stressed leaves of Yukon were 58.3% higher than on Shandong. Water deficit caused a 28.2% increase in total cutin monomers on Shandong, whereas total cutin monomers were not induced on Yukon. With or without stress, more abundant cuticle lipids were generally associated with lower water loss rates, lower chlorophyll efflux rates and an extended time before water deficit-induced wilting. In response to water deficit, Shandong showed elevated transcription of genes encoding elongase subunits, consistent with the higher stress induction of acids by Shandong. Yukon's higher induction of CER1 and CER3 transcripts may explain why alkanes increased most on Yukon after water deficit. Eutrema, with its diverse cuticle lipids and responsiveness, provides a valuable genetic resource for identifying new genes and alleles effecting cuticle metabolism, and lays groundwork for studies of the cuticle's role in extreme stress tolerance. C1 [Xu, Xiaojing; Feng, Jinchao; An, Huiling; Zhou, Yijun] Minzu Univ China, Coll Life & Environm Sci, Dept Biol Sci, Beijing 100081, Peoples R China. [Lu, Shiyou] Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China. [Lohrey, Greg T.; Jenks, Matthew A.] ARS, US Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. RP Jenks, MA (reprint author), ARS, US Arid Land Agr Res Ctr, USDA, Maricopa, AZ 85138 USA. EM matt.jenks@ars.usda.gov FU National Natural Science Foundation of China [31100287]; '985 Project' of Minzu University of China [MUC98504-14, MUC98507-08]; Fundamental Research Funds for the Central Universities [1112KYZY43] FX This work was funded by National Natural Science Foundation of China (31100287), '985 Project' of Minzu University of China (MUC98504-14, MUC98507-08) and Fundamental Research Funds for the Central Universities (1112KYZY43). NR 43 TC 10 Z9 11 U1 0 U2 25 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0031-9317 EI 1399-3054 J9 PHYSIOL PLANTARUM JI Physiol. Plant. PD AUG PY 2014 VL 151 IS 4 BP 446 EP 458 DI 10.1111/ppl.12127 PG 13 WC Plant Sciences SC Plant Sciences GA AN0DU UT WOS:000340253700007 PM 24215503 ER PT J AU Gordon, SP Priest, H Marais, DLD Schackwitz, W Figueroa, M Martin, J Bragg, JN Tyler, L Lee, CR Bryant, D Wang, WQ Messing, J Manzaneda, AJ Barry, K Garvin, DF Budak, H Tuna, M Mitchell-Olds, T Pfender, WF Juenger, TE Mockler, TC Vogel, JP AF Gordon, Sean P. Priest, Henry Marais, David L. Des Schackwitz, Wendy Figueroa, Melania Martin, Joel Bragg, Jennifer N. Tyler, Ludmila Lee, Cheng-Ruei Bryant, Doug Wang, Wenqin Messing, Joachim Manzaneda, Antonio J. Barry, Kerrie Garvin, David F. Budak, Hikmet Tuna, Metin Mitchell-Olds, Thomas Pfender, William F. Juenger, Thomas E. Mockler, Todd C. Vogel, John P. TI Genome diversity in Brachypodium distachyon: deep sequencing of highly diverse inbred lines SO PLANT JOURNAL LA English DT Article DE Brachypodium distachyon; natural diversity; genome sequencing; transcriptome; drought ID ARABIDOPSIS-THALIANA; GENE-EXPRESSION; MODEL SYSTEM; SHORT READS; DROUGHT; STRESS; RICE; OVEREXPRESSION; POPULATIONS; PATTERNS AB Brachypodium distachyon is small annual grass that has been adopted as a model for the grasses. Its small genome, high-quality reference genome, large germplasm collection, and selfing nature make it an excellent subject for studies of natural variation. We sequenced six divergent lines to identify a comprehensive set of polymorphisms and analyze their distribution and concordance with gene expression. Multiple methods and controls were utilized to identify polymorphisms and validate their quality. mRNA-Seq experiments under control and simulated drought-stress conditions, identified 300 genes with a genotype-dependent treatment response. We showed that large-scale sequence variants had extremely high concordance with altered expression of hundreds of genes, including many with genotype-dependent treatment responses. We generated a deep mRNA-Seq dataset for the most divergent line and created a de novo transcriptome assembly. This led to the discovery of >2400 previously unannotated transcripts and hundreds of genes not present in the reference genome. We built a public database for visualization and investigation of sequence variants among these widely used inbred lines. C1 [Gordon, Sean P.; Bragg, Jennifer N.; Vogel, John P.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. [Priest, Henry; Bryant, Doug; Mockler, Todd C.] Donald Danforth Plant Sci Ctr, St Louis, MO 63132 USA. [Marais, David L. Des; Juenger, Thomas E.] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. [Schackwitz, Wendy; Martin, Joel; Barry, Kerrie] DOE Joint Genome Inst, Walnut Creek, CA 94598 USA. [Figueroa, Melania; Pfender, William F.] USDA ARS, Corvallis, OR 97331 USA. [Figueroa, Melania; Pfender, William F.] Univ Minnesota, St Paul, MN 55108 USA. [Bragg, Jennifer N.] Univ Calif Davis, Davis, CA 95616 USA. [Tyler, Ludmila] Univ Massachusetts, Amherst, MA 01003 USA. [Lee, Cheng-Ruei; Mitchell-Olds, Thomas] Duke Biol, Durham, NC 27708 USA. [Wang, Wenqin; Messing, Joachim] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA. [Manzaneda, Antonio J.] Univ Jaen, Jaen 23071, Spain. [Garvin, David F.] USDA ARS, Plant Sci Res Unit, St Paul, MN 55108 USA. [Budak, Hikmet] Sabanci Univ, TR-34956 Orhanli, Tuzla Istanbul, Turkey. [Tuna, Metin] Namik Kemal Univ, Dept Field Crops, Tekirdag 59030, Turkey. [Pfender, William F.] Oregon State Univ, Corvallis, OR 97331 USA. RP Vogel, JP (reprint author), USDA ARS, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM brachypodium@gmail.com RI Budak, Hikmet/F-4708-2010; Mockler, Todd/L-2609-2013; Mitchell-Olds, Thomas/K-8121-2012; OI Vogel, John/0000-0003-1786-2689; Budak, Hikmet/0000-0002-2556-2478; Mockler, Todd/0000-0002-0462-5775; Wang, Wenqin/0000-0001-6427-6338; Mitchell-Olds, Thomas/0000-0003-3439-9921; Martin, Joel/0000-0001-9511-6441; Manzaneda Avila, Antonio Jose/0000-0001-9384-7910 FU Office of Science (BER), US Department of Energy, USDA NIFA; USDA CRIS [5325-21000-017-00] FX We thank Uffe Hellsten for help with population genetics. Genomic DNA sequencing was performed at the US Department of Energy Joint Genome Institute through the Community Sequencing Program. Deep sequencing of the Bd1-1 transcriptome was performed at the Center of Genome Research and Biocomputing, Oregon State University, Corvallis, OR. This work was supported by the Office of Science (BER), US Department of Energy, USDA NIFA, and by the USDA CRIS project 5325-21000-017-00. NR 49 TC 21 Z9 21 U1 1 U2 42 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0960-7412 EI 1365-313X J9 PLANT J JI Plant J. PD AUG PY 2014 VL 79 IS 3 BP 361 EP 374 DI 10.1111/tpj.12569 PG 14 WC Plant Sciences SC Plant Sciences GA AM8XV UT WOS:000340163500001 PM 24888695 ER PT J AU Sun, C VanRaden, PM Cole, JB O'Connell, JR AF Sun, Chuanyu VanRaden, Paul M. Cole, John B. O'Connell, Jeffrey R. TI Improvement of Prediction Ability for Genomic Selection of Dairy Cattle by Including Dominance Effects SO PLOS ONE LA English DT Article ID BREEDING VALUE PREDICTION; MILK-PRODUCTION TRAITS; METHOD-R; VARIANCE; INFORMATION; PEDIGREE; VALUES; YIELD; POPULATION; REGRESSION AB Dominance may be an important source of non-additive genetic variance for many traits of dairy cattle. However, nearly all prediction models for dairy cattle have included only additive effects because of the limited number of cows with both genotypes and phenotypes. The role of dominance in the Holstein and Jersey breeds was investigated for eight traits: milk, fat, and protein yields; productive life; daughter pregnancy rate; somatic cell score; fat percent and protein percent. Additive and dominance variance components were estimated and then used to estimate additive and dominance effects of single nucleotide polymorphisms (SNPs). The predictive abilities of three models with both additive and dominance effects and a model with additive effects only were assessed using ten-fold cross-validation. One procedure estimated dominance values, and another estimated dominance deviations; calculation of the dominance relationship matrix was different for the two methods. The third approach enlarged the dataset by including cows with genotype probabilities derived using genotyped ancestors. For yield traits, dominance variance accounted for 5 and 7% of total variance for Holsteins and Jerseys, respectively; using dominance deviations resulted in smaller dominance and larger additive variance estimates. For non-yield traits, dominance variances were very small for both breeds. For yield traits, including additive and dominance effects fit the data better than including only additive effects; average correlations between estimated genetic effects and phenotypes showed that prediction accuracy increased when both effects rather than just additive effects were included. No corresponding gains in prediction ability were found for non-yield traits. Including cows with derived genotype probabilities from genotyped ancestors did not improve prediction accuracy. The largest additive effects were located on chromosome 14 near DGAT1 for yield traits for both breeds; those SNPs also showed the largest dominance effects for fat yield (both breeds) as well as for Holstein milk yield. C1 [Sun, Chuanyu] Natl Assoc Anim Breeders, Columbia, MO USA. [VanRaden, Paul M.; Cole, John B.] USDA, Agr Res Serv, Anim Genom & Improvement Lab, Beltsville, MD USA. [O'Connell, Jeffrey R.] Univ Maryland, Sch Med, Baltimore, MD 21201 USA. RP Sun, C (reprint author), Natl Assoc Anim Breeders, Columbia, MO USA. EM Chuanyu.Sun@ars.usda.gov RI Cole, John/J-8571-2014 OI Cole, John/0000-0003-1242-4401 FU National Association of Animal Breeders, United States of America; Animal Improvement Programs Laboratory of Agricultural research service in the United States Department of Agriculture FX The work was performed in the project funded regularly by National Association of Animal Breeders, United States of America (http://www.naab-css.org/) and Animal Improvement Programs Laboratory of Agricultural research service in the United States Department of Agriculture (http://aipl.arsusda.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 38 TC 18 Z9 18 U1 0 U2 17 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD AUG 1 PY 2014 VL 9 IS 8 AR e103934 DI 10.1371/journal.pone.0103934 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AM4JL UT WOS:000339819800105 PM 25084281 ER PT J AU Olanrewaju, HA Miller, WW Maslin, WR Collier, SD Purswell, JL Branton, SL AF Olanrewaju, H. A. Miller, W. W. Maslin, W. R. Collier, S. D. Purswell, J. L. Branton, S. L. TI Effects of strain and light intensity on growth performance and carcass characteristics of broilers grown to heavy weights SO POULTRY SCIENCE LA English DT Article DE genetic strain; light intensity; growth; meat yield; broiler ID LIVE PERFORMANCE; PROCESSING CHARACTERISTICS; MEAT QUALITY; LEG HEALTH; CHICKENS; PHOTOPERIOD; WELFARE; RESPONSES; GENOTYPES; CROSSES AB Effects of genetic strain and light intensity on growth performance and carcass characteristics of broilers grown to heavy weights were investigated. The experimental design was a randomized complete block design. Treatment structure was a 2 x 5 factorial arrangement with the main factors being strain (Ross x Ross 308, Ross x Ross 708) and light intensity (25, 10, 5, 2.5, and 0.2 lx) with trial as replicates. In each of the 5 trials, chicks of 2 different strains of the same commercial hatchery were equally and randomly distributed into 10 environmentally controlled rooms (5 rooms/strain) at 1 d of age at 50% RH. Each room was randomly assigned 1 of 5 light intensities from 22 to 56 d of age. Feed and water were provided ad libitum. Birds and feed were weighed on 0, 14, 28, 42, and 56 d of age for growth performance. Humoral immune response was determined on d 28, whereas ocular and blood samples were performed on d 42 and 55, respectively. On d 56, 20 (10 males and 10 females) birds/strain from each room were processed to determine weights and yields. Genetic strain was significant (P <= 0.05) for most of the examined variables, where Ross x Ross 308 had better growth performance and meat yield in comparison with Ross x Ross 708. Although, there was no main effect of light intensity on growth performance and meat yield, results indicated that birds under 10 and 5 lx intensities showed slightly better growth performance and meat yield compared with birds under 25, 2.5, and 0.2 lx in both strains. There was no effect of strain and light intensity on ocular indices, immune response, plasma corticosterone levels, and mortality. This study shows the positive influence on profits to commercial poultry facilities that are using a low lighting environment to reduce energy cost, optimize feed conversion, and maximize production without compromising the welfare of the broilers. C1 [Olanrewaju, H. A.; Collier, S. D.; Purswell, J. L.; Branton, S. L.] USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. [Miller, W. W.] Adv Anim Eye Care, Starkville, MS 39759 USA. [Maslin, W. R.] Mississippi State Univ, Wise Ctr, Coll Vet Med, Mississippi State, MS 39762 USA. RP Olanrewaju, HA (reprint author), USDA ARS, Poultry Res Unit, POB 5397, Mississippi State, MS 39762 USA. EM Hammed.Olanrewaju@ars.usda.gov NR 47 TC 2 Z9 2 U1 6 U2 23 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2014 VL 93 IS 8 BP 1890 EP 1899 DI 10.3382/ps.2013-03806 PG 10 WC Agriculture, Dairy & Animal Science SC Agriculture GA AM7IY UT WOS:000340041100003 PM 24894522 ER PT J AU Webb, ML Spickler, JL Bourassa, DV Cox, NA Wilson, JL Buhr, RJ AF Webb, M. L. Spickler, J. L. Bourassa, D. V. Cox, N. A. Wilson, J. L. Buhr, R. J. TI Recovery of Salmonella serovar Enteritidis from inoculated broiler hatching eggs using shell rinse and shell crush sampling methods SO POULTRY SCIENCE LA English DT Article DE broiler hatching egg; shell crush; eggshell Salmonella; shell rinse; Salmonella inoculation ID ANTIMICROBIAL ACTIVITY; BACTERIAL PENETRATION; HENS EGG; CUTICLE; TYPHIMURIUM; MEMBRANES; LAYER; TRANSMISSION; INFECTIONS; EGGSHELLS AB This study compared the recovery of Salmonella from hatching eggs using 3 sampling methods (eggshell rinsing, eggshell crush following a previous rinse, and eggshell crush without previous rinse). Eggshells were drop-inoculated with approximately 10(1), 10(2), or 10(3) cfu/eggshell of Salmonella Enteritidis and allowed to dry at room temperature for 1 or 24 h. For the shell rinse groups, each inoculated egg was rinsed with buffered peptone water. These rinsed eggs were used for the shell crush with previous rinse groups, and each egg was aseptically cracked, the contents discarded, and the eggshell and membranes crushed with buffered peptone water. This same crush procedure was used for the shell crush without previous shell rinse eggs. The recovery of Salmonella 1 h after inoculation for shell rinse sampled eggs was 16% positive at 10(1), 49% at 10(2), and 93% at 10(3) cfu/eggshell challenge. For the shell crush with previous shell rinse, sampled egg recovery was 0% positive at 10(1), 3% at 10(2), and 17% at 10(3) cfu/eggshell. For the shell crush, sampled eggs had recovery of 23% positive at 10(1), 69% at 10(2), and 96% at 10(3) cfu/eggshell challenge. The recovery of Salmonella 24 h after inoculation for the shell rinse eggs was 3% positive at 10(1), 12% at 10(2), and 22% at 10(3) cfu/eggshell challenge; recovery for shell crush with previous shell rinse sampling was 2% positive at 10(1), 8% at 10(2), and 5% at 10(3) cfu/eggshell challenge; and for the shell crush sampling recovery was 2% at 10(1), 32% at 10(2), and 42% at 10(3) cfu/eggshell challenge. Eggshell crush was a more sensitive (similar to 10 percentage points) sampling method than eggshell rinse at both 1 and 24 h, but both methods were equally optimal when the inoculum was at 10(3) and samples were collected after 1 h. Waiting 24 h after inoculation to sample significantly lowered the recovery for both the shell rinse and shell crush sampling methods by similar to 40 percentage points. C1 [Webb, M. L.] Claflin Univ, Dept Biol, Orangeburg, SC 29115 USA. [Webb, M. L.; Spickler, J. L.; Bourassa, D. V.; Cox, N. A.; Buhr, R. J.] USDA, Agr Res Serv, Richard B Russell Agr Res Ctr, Poultry Microbiol Safety Res Unit, Athens, GA 30605 USA. [Spickler, J. L.; Wilson, J. L.] Univ Georgia, Dept Poultry Sci, Athens, GA 30602 USA. RP Buhr, RJ (reprint author), USDA, Agr Res Serv, Richard B Russell Agr Res Ctr, Poultry Microbiol Safety Res Unit, Athens, GA 30605 USA. EM jeff.buhr@ars.usda.gov NR 40 TC 0 Z9 0 U1 1 U2 9 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2014 VL 93 IS 8 BP 2117 EP 2122 DI 10.3382/ps.2014-03992 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA AM7IY UT WOS:000340041100030 PM 24931964 ER PT J AU Kim, EJ Purswell, JL Evans, JD Branton, SL AF Kim, E. J. Purswell, J. L. Evans, J. D. Branton, S. L. TI Production characteristics of Hy-Line W36 laying hens hatched from white and tinted eggs SO POULTRY SCIENCE LA English DT Article DE layer; egg production; shell color ID COLOR; QUALITY; INHERITANCE AB Eggshell color can greatly influence visual appeal of table eggs, and within the United States, table eggs are normally sorted and marked according to eggshell color to maximize consumer appeal. Recently, table egg producers have noted increased incidence of "off-color" or tinted (TT) eggs derived from white egg laying breeder hens. Therefore, the objectives of this study were to determine the production characteristics and resultant eggshell color of laying hens hatched from different colored eggs. Hy-Line W36 eggs were obtained from a commercial breeder operation and eggshell color was assessed with a colorimeter to separate eggs into groups of tinted (TT) and nontinted (NT) eggs before incubation. Treatment groups were placed into separate hatching trays. At hatching, chicks from each treatment group were individually wing-banded. Pullets were randomly allocated into cages according to treatment groups at 18 wk. Birds were placed into individual cages, with 5 consecutive cages representing a treatment replicate. Each treatment was replicated 24 times for a total of 120 birds per treatment and fed a nutritionally complete layer diet. Production performance was evaluated from 18 to 34 wk of age. Average weekly egg production was calculated. Feed intake, egg weights, egg mass, feed conversion ratio, and egg color were analyzed every 2 wk. Birds were weighed every 4 wk until completion of the study. Birds hatched from TT eggs had significantly increased BW throughout the experimental period. Hen-day egg production was significantly different when compared with the NT treatment at 19 and 20 wk of age. Eggshell color was also found to be significantly different for the NT and TT groups with TT eggs being significantly further from true white. Selection of progeny based on eggshell color may be a criterion for selecting white egg layers as layers hatched from TT eggs resulted in more off-color eggs, which may affect consumer acceptance for buying white table eggs. C1 [Kim, E. J.; Purswell, J. L.; Evans, J. D.; Branton, S. L.] USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. RP Kim, EJ (reprint author), USDA ARS, Poultry Res Unit, Mississippi State, MS 39762 USA. EM Elizabeth.Kim@ars.usda.gov NR 14 TC 0 Z9 0 U1 0 U2 6 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0032-5791 EI 1525-3171 J9 POULTRY SCI JI Poult. Sci. PD AUG PY 2014 VL 93 IS 8 BP 2123 EP 2128 DI 10.3382/ps.2013-03647 PG 6 WC Agriculture, Dairy & Animal Science SC Agriculture GA AM7IY UT WOS:000340041100031 PM 24931965 ER PT J AU Hatfield, DP Sweeney, KP Lau, J Lichtenstein, AH AF Hatfield, Daniel P. Sweeney, Kathryn P. Lau, Joseph Lichtenstein, Alice H. TI Critical assessment of high-circulation print newspaper coverage of the Institute of Medicine report Dietary Reference Intakes for Calcium and Vitamin D SO PUBLIC HEALTH NUTRITION LA English DT Article DE Vitamin D; Calcium; Recommended dietary allowances; Institute of Medicine; Newspaper; Media ID MEDIA; JOURNALISTS; ASSOCIATION; PREVENTION; ATTITUDES; NEWS AB Objective: To evaluate high-circulation US and Canadian newspaper coverage of the Institute of Medicine (IOM) report Dietary Reference Intakes for Calcium and Vitamin D and assess pre-report and post-report reporter-specific vitamin D-related coverage. Design: Two independent reviewers analysed the newspaper articles. The key report findings cited, proportion of sentences describing the IOM report and proportion of sentences describing critical viewpoints on the report were calculated. The content of articles written by reporters with a history of pre-report vitamin D-related articles was compared with that of articles written by reporters without such a history. Setting: Factiva and LexisNexis searches of the top thirty US and three English-language Canadian print newspapers, by circulation. Subjects: Articles on the IOM report published from 30 November to 21 December 2010 and previous vitamin D-related articles written by the same reporters. Results: Only ten articles met inclusion/exclusion criteria. Articles inconsistently cited the key findings in the IOM report. Reporters with a history of pre-report articles highlighting the benefits of vitamin D dedicated a greater proportion of sentences to viewpoints critical of the IOM report (P<0.01). There was no significant difference between pre-report publication history and proportion of sentences focused on the IOM report. A borderline-significant difference (P=0.058) was observed between pre-report articles highlighting the benefits of vitamin D and the absence of reference to potential risks of vitamin D overconsumption. Conclusion: Our findings suggest that newspaper articles did not consistently or comprehensively report the IOM recommendations and that pre-report publication history of reporters was related to post-report article content. C1 [Hatfield, Daniel P.; Sweeney, Kathryn P.; Lau, Joseph; Lichtenstein, Alice H.] Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Pol, Boston, MA 02111 USA. [Hatfield, Daniel P.; Lau, Joseph; Lichtenstein, Alice H.] Tufts Med Ctr, Inst Clin Res & Hlth Policy Studies, Ctr Clin Evidence Synth, Boston, MA USA. [Lichtenstein, Alice H.] Tufts Univ, Jean Mayer US Dept Agr, Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Gerald J & Dorothy R Friedman Sch Nutr Sci & Pol, 150 Harrison Ave, Boston, MA 02111 USA. EM Alice.Lichtenstein@Tufts.edu OI Hatfield, Daniel/0000-0001-9315-0419 NR 39 TC 1 Z9 1 U1 2 U2 8 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1368-9800 EI 1475-2727 J9 PUBLIC HEALTH NUTR JI Public Health Nutr. PD AUG PY 2014 VL 17 IS 8 BP 1868 EP 1876 DI 10.1017/S1368980013002073 PG 9 WC Public, Environmental & Occupational Health; Nutrition & Dietetics SC Public, Environmental & Occupational Health; Nutrition & Dietetics GA AM8YF UT WOS:000340164600023 PM 23902633 ER PT J AU Weber, JG AF Weber, Jeremy G. TI A decade of natural gas development: The makings of a resource curse? SO RESOURCE AND ENERGY ECONOMICS LA English DT Article DE Natural gas development; Resource curse; Multiplier ID SPATIAL ECONOMETRICS; EMPLOYMENT; ECONOMY; BOOM AB Many studies find that areas more dependent on natural resources grow more slowly - a relationship known as the resource curse. For counties in the south-central U.S., I find little evidence of an emerging curse from greater natural gas production in the 2000s. Each gas-related mining job created more than one nonmining job, indicating that counties did not become more dependent on mining as measured by employment. Increases in population largely mitigated a rise in earnings per job and crowding out. Furthermore, changes in the adult population by education level reveal that greater production did not lead to a less educated population. Published by Elsevier B.V. C1 ERS, USDA, Washington, DC 20250 USA. RP Weber, JG (reprint author), ERS, USDA, 1400 Independence Ave SW,Mail Stop 1800, Washington, DC 20250 USA. EM jeweber@ers.usda.gov NR 42 TC 20 Z9 20 U1 1 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0928-7655 EI 1873-0221 J9 RESOUR ENERGY ECON JI Resour. Energy Econ. PD AUG PY 2014 VL 37 BP 168 EP 183 DI 10.1016/j.reseneeco.2013.11.013 PG 16 WC Economics; Energy & Fuels; Environmental Sciences; Environmental Studies SC Business & Economics; Energy & Fuels; Environmental Sciences & Ecology GA AN1FZ UT WOS:000340329400009 ER PT J AU Fackler, PL Haight, RG AF Fackler, Paul L. Haight, Robert G. TI Monitoring as a partially observable decision problem SO RESOURCE AND ENERGY ECONOMICS LA English DT Article DE Environmental monitoring; Dynamic programming; Partial observability; Value of information; POMDP ID MANAGEMENT; ALGORITHMS; UNCERTAIN; APPROXIMATIONS; CONSERVATION; RESOURCES; POLICIES; POMDPS; MODELS; PLANT AB Monitoring is an important and costly activity in resource management problems such as containing invasive species, protecting endangered species, preventing soil erosion, and regulating contracts for environmental services. Recent studies have viewed optimal monitoring as a Partially Observable Markov Decision Process (POMDP), which provides a framework for sequential decision making under stochastic resource dynamics and uncertainty about the resource state. We present an overview of the POMDP framework and its applications to resource monitoring. We discuss the concept of the information content provided by monitoring systems and illustrate how information content affects optimal monitoring strategies. Finally, we demonstrate how the timing of monitoring in relation to resource treatment and transition can have substantial effects on optimal monitoring strategies. (C) 2014 Elsevier B.V. All rights reserved. C1 [Fackler, Paul L.] N Carolina State Univ, Dept Agr & Resource Econ, Raleigh, NC 27695 USA. [Haight, Robert G.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP Fackler, PL (reprint author), N Carolina State Univ, Dept Agr & Resource Econ, Box 8109, Raleigh, NC 27695 USA. EM paul_fackler@ncsu.edu; rhaight@fs.fed.us NR 37 TC 3 Z9 3 U1 5 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0928-7655 EI 1873-0221 J9 RESOUR ENERGY ECON JI Resour. Energy Econ. PD AUG PY 2014 VL 37 BP 226 EP 241 DI 10.1016/j.reseneeco.2013.12.005 PG 16 WC Economics; Energy & Fuels; Environmental Sciences; Environmental Studies SC Business & Economics; Energy & Fuels; Environmental Sciences & Ecology GA AN1FZ UT WOS:000340329400012 ER PT J AU Diaz-Montano, J Vindiola, BG Drew, N Novy, RG Miller, JC Trumble, JT AF Diaz-Montano, John Vindiola, Beatriz G. Drew, Nichole Novy, Richard G. Miller, J. Creighton, Jr. Trumble, John T. TI Resistance of Selected Potato Genotypes to the Potato Psyllid (Hemiptera: Triozidae) SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Bactericera cockerelli; Solanum tuberosum; Antibiosis; Antixenosis ID BACTERICERA-COCKERELLI SULC; NEW-ZEALAND; ZEBRA CHIP; CROPS; HOMOPTERA; DISEASES; TOMATO; INSECTICIDES; MEXICO AB The characterization of resistance of selected potato, Solanum tuberosum L., breeding clones to the potato psyllid, Bactericera cockerelli (Sulc) (Hemiptera: Triozidae) was investigated. Antixenosis was assessed in choice tests in which a single plant of each genotype was placed inside a rearing cage, where 60 female psyllid adults were released and the number of adults and eggs on each genotype was counted 24 h later. Antibiosis was evaluated in no-choice tests in which adults (five males and five females) were confined in a cage fixed to the upper side of leaves. After 4 h of exposure, adults were removed and the number of eggs counted. The developmental time and survival of offspring were recorded until all insects became adults. All the resistant genotypes showed strong antibiotic effects to B. cockerelli. These results show promise for incorporation into an IPM program against B. cockerelli. C1 [Diaz-Montano, John; Vindiola, Beatriz G.; Drew, Nichole; Trumble, John T.] Univ Calif Riverside, Dept Entomol, Riverside, CA 92521 USA. [Novy, Richard G.] ARS, Small Grains & Potato Germplasm Res Unit, USDA, Aberdeen, ID 83210 USA. [Miller, J. Creighton, Jr.] Texas A&M Univ, Dept Hort Sci, College Stn, TX 77843 USA. RP Diaz-Montano, J (reprint author), ARS, Stored Product Insect Res Unit, USDA, Ctr Grain & Anim Hlth Res, 1515 Coll Av, Manhattan, KS 66502 USA. EM john.diaz-montano@ars.usda.gov FU USDA SCRI grant [2009-34381-20036]; USDA RAMP grant [2009-51101-05892]; California Potato Board FX We thank Bill Carson, Greg Kund, Deborah De La Riva, Kristen Hladun, Christina Mogren, Sammuel Gilbert, Khoa Tran (Chris), Kristine Gilbert and Lindsay Johnson for helping in different aspects of this study. This research was partially funded by a USDA SCRI grant (2009-34381-20036), a USDA RAMP grant (2009-51101-05892) and the California Potato Board. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture, Texas A & M University or the University of California-Riverside. USDA is an equal opportunity provider and employer. NR 24 TC 5 Z9 5 U1 2 U2 22 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2014 VL 91 IS 4 BP 363 EP 367 DI 10.1007/s12230-013-9356-6 PG 5 WC Agronomy SC Agriculture GA AM3BL UT WOS:000339726300005 ER PT J AU Bamberg, J del Rio, A AF Bamberg, John del Rio, Alfonso TI Selection and Validation of an AFLP Marker Core Collection for the Wild Potato Solanum microdontum SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Core; Microdontum; Genebank ID PETOTA AB Solanum microdontum is a diploid potato species with features that make it a good model for research into management and use of germplasm in the genebank. Its taxonomic status is unambiguous and it is in the taxonomic series of wild species closest to cultivated forms. It is represented by about 100 populations in the genebank-not too many for comprehensive evaluation, yet not too few to make prioritization of the most valuable populations worthwhile. This species is also particularly rich in desirable traits, often exhibiting very broad segregation. We here report use of DNA markers for selection of a core set of populations, and assessing whether that core captures populations with the most desirable evaluation results for economic traits. DNA was extracted from bulks of 27 plants from each of 94 populations to generate AFLPs. A total of 1,741 informative loci were detected. AFLP loci were treated as though they were traits, with the banded condition considered to be the desired state to include in a core set. At least one band unique to a population was present in 45 populations, and these 45 populations together captured 98 % of all bands. Adding another 14 populations for a core of 59 populations captured 100 % of bands. This core set was assessed for whether it encompassed those populations known to have useful traits, including nutritional and quality components; and disease, stress and pest resistances. As with AFLP bands, 25 of 26 of the most desirable phenotypic traits were also found in populations in the core set of 59 populations. The most desirable status of 3 traits is lost by selecting a core of 45 populations. We conclude that these core sets would be a rational starting point when prospecting for new useful traits in microdontum. C1 [Bamberg, John; del Rio, Alfonso] ARS, USDA, US Potato Genebank, Sturgeon Bay, WI 54235 USA. RP Bamberg, J (reprint author), ARS, USDA, US Potato Genebank, Sturgeon Bay, WI 54235 USA. EM John.Bamberg@ars.usda.gov OI Del Rio, Alfonso/0000-0001-8780-747X NR 15 TC 6 Z9 6 U1 0 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2014 VL 91 IS 4 BP 368 EP 375 DI 10.1007/s12230-013-9357-5 PG 8 WC Agronomy SC Agriculture GA AM3BL UT WOS:000339726300006 ER PT J AU Novy, RG Whitworth, JL Stark, JC Charlton, BA Yilma, S Knowles, NR Pavek, MJ Spear, RR Brandt, TL Olsen, N Thornton, M Brown, CR AF Novy, R. G. Whitworth, J. L. Stark, J. C. Charlton, B. A. Yilma, S. Knowles, N. R. Pavek, M. J. Spear, R. R. Brandt, T. L. Olsen, N. Thornton, M. Brown, C. R. TI Teton Russet: An Early-Maturing, Dual-Purpose Potato Cultivar Having Higher Protein and Vitamin C Content, Low Asparagine, and Resistances to Common Scab and Fusarium Dry Rot SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Breeding; Variety; Processing; Fresh pack; Common scab resistance; Fusarium dry rot resistance; Asparagine; Acrylamide ID FRESH-MARKET; LATE BLIGHT; GERMPLASM; VARIETIES; TRIALS AB Teton Russet is an early-maturing, medium-russeted, potato cultivar with high merit for both fresh-pack and processing. In early harvest trials in the Pacific Northwest, Teton Russet had total yields similar to Russet Norkotah, and higher than Ranger Russet and Russet Burbank. Marketable yield of Teton Russet in the early harvest trials was also comparable to or higher than Russet Norkotah in Washington and Oregon, and higher than Ranger Russet and Russet Burbank at these sites, as well as in Idaho. In full-season trials, while total yield of the earlier-maturing Teton Russet tended to be lower than Ranger Russet and Russet Burbank, marketable yield was generally higher than Russet Burbank across the majority of sites due to its higher percentage of U.S. No. 1 tubers. Teton Russet is suitable for processing, with acceptable fry color following up to 8 months of storage at 8.9 A degrees C. Uniformity of fry color was also very consistent. Teton Russet has shown lower levels of the amino acid asparagine relative to Ranger Russet and Russet Burbank which may contribute to lower acrylamide levels in French fries and other processed potato products. Teton Russet is notable for having resistance to common scab (Streptomyces spp.) and Fusarium dry rot, and is moderately resistant to tuber net necrosis. Analyses have also shown Teton Russet to have significantly higher protein levels than Russet Norkotah, Ranger Russet, and Russet Burbank, as well as higher vitamin C content than Russet Norkotah and Russet Burbank. Teton Russet was released in 2011 by the USDA-ARS and the Agricultural Experiment Stations of Idaho, Oregon, and Washington, and is a product of the Pacific Northwest Potato Variety (Tri-State) Development Program. C1 [Novy, R. G.; Whitworth, J. L.] ARS, USDA, Aberdeen Res & Extens R&E Ctr, Aberdeen, ID 83210 USA. [Stark, J. C.] Univ Idaho, Idaho Falls R&E Ctr, Idaho Falls, ID 83402 USA. [Charlton, B. A.] Oregon State Univ, Klamath Basin R&E Ctr, Klamath Falls, OR 97603 USA. [Yilma, S.] Oregon State Univ, Corvallis, OR 97331 USA. [Knowles, N. R.; Pavek, M. J.; Spear, R. R.] Washington State Univ, Pullman, WA 99164 USA. [Brandt, T. L.; Olsen, N.] Univ Idaho, Kimberly R&E Ctr, Kimberly, ID 83341 USA. [Thornton, M.] Univ Idaho, Southwest Idaho R&E Ctr, Parma, ID 83660 USA. [Brown, C. R.] ARS, USDA, Prosser, WA 99350 USA. RP Novy, RG (reprint author), ARS, USDA, Aberdeen Res & Extens R&E Ctr, Aberdeen, ID 83210 USA. EM Rich.Novy@ars.usda.gov FU USDA/CSREES Special Potato Program Grant FX The authors thank Margaret Bain, William Buhrig, Mel Chapel, Todd Carter, Mary Jo Frazier, Nora Fuller, Darren Hall, Mark Fristad, Teri Hill, Zach Holden, Chelsey Lowder, Charlene Miller, Bart Nelson, Brian Schneider, Lura Schroeder, Penny Tubbs, Rich Quick, Steve Wheeler, and Jim Whitmore for their contributions to the development and release of Teton Russet, as well as Shelley Jansky for her review and suggestions for improvement of the manuscript prior to submission for publication. We also thank Lori Ewing, University of Idaho, for her efforts in establishing pathogen-free in vitro plantlets of Teton Russet, as well as our industry cooperators, our collaborators in the Western Regional Potato Variety Trials, and the Idaho, Washington, and Oregon potato commissions. Development of Teton Russet was partially funded by the USDA/CSREES Special Potato Program Grant. NR 25 TC 0 Z9 0 U1 3 U2 20 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2014 VL 91 IS 4 BP 380 EP 393 DI 10.1007/s12230-013-9362-8 PG 14 WC Agronomy SC Agriculture GA AM3BL UT WOS:000339726300008 ER PT J AU Hutchinson, PJS Felix, J Boydston, R AF Hutchinson, Pamela J. S. Felix, Joel Boydston, Rick TI Glyphosate Carryover in Seed Potato: Effects on Mother Crop and Daughter Tubers SO AMERICAN JOURNAL OF POTATO RESEARCH LA English DT Article DE Solanum tuberosum; Glyphosate; Application timing; Rate; Mother crop; Daughter tuber ID SOLANUM-TUBEROSUM; SIMULATED DRIFT; DOSE-RESPONSE; GLUFOSINATE; HERBICIDES; IMPACTS; SEASON; PLANTS AB Field studies were conducted in 2008 and 2009 in Aberdeen, ID, Ontario, OR, and Paterson, WA to determine the effect of simulated glyphosate drift on 'Ranger Russet' potato during the application year and the crop growing the next year from the daughter tubers. Glyphosate was applied at 8.5, 54, 107, 215, and 423 g ae ha(-1) which corresponds to 1/00, 1/16, 1/8, A1/4, and 1/2 of the lowest recommended single-application rate for glyphosate-resistant corn and sugar beet of 846 g ha(-1). Glyphosate was applied when potato plants were at 10 to 15 cm tall (Early), or at stolon hooking (H), tuber initiation (TI), or during mid-bulking (MB). In general, the MB applications caused less visual foliar injury to the mother crop than earlier applications at ID or OR, and H applications at WA. Mother crop injury increased as glyphosate rate increased regardless of location, application timing, and rating date. U.S. No.1 and total tuber yields were usually related to the injury level resulting from glyphosate application timings and rates. Although injury to the mother crop from glyphosate applied at MB usually was the lowest compared to injury from other application timings, when daughter tubers from that timing were planted the following year, emergence, plant vigor, and yield was most detrimentally impacted compared with that of daughter tubers from other timing treatments. MB daughter tuber emergence was less than 30 % of the nontreated control tuber emergence while emergence of daughter tubers from the other treatments was 60 to 95 %. As rate of glyphosate applied to the mother crop increased, daughter tuber emergence decreased. When MB daughter tubers did emerge, plants were chlorotic and stunted as if the plants had been directly sprayed with glyphosate. Regardless of whether the daughter tubers had defects or not, results the following year were the same. Implications are that if a mother seed crop encounters glyphosate during bulking, injury may not even be noticeable on the foliage or the tubers, however, emergence, vigor, and yield of the crop growing the following year from the daughter tubers could be greatly impacted. C1 [Hutchinson, Pamela J. S.] Univ Idaho, Aberdeen Res & Extens Ctr, Aberdeen, ID 83210 USA. [Felix, Joel] Oregon State Univ, Malheur Expt Stn, Ontario, OR 97914 USA. [Boydston, Rick] ARS, USDA, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA. RP Hutchinson, PJS (reprint author), Univ Idaho, Aberdeen Res & Extens Ctr, 1693 S 2700 W, Aberdeen, ID 83210 USA. EM phutch@uidaho.edu NR 34 TC 2 Z9 3 U1 3 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1099-209X EI 1874-9380 J9 AM J POTATO RES JI Am. J. Potato Res. PD AUG PY 2014 VL 91 IS 4 BP 394 EP 403 DI 10.1007/s12230-013-9363-7 PG 10 WC Agronomy SC Agriculture GA AM3BL UT WOS:000339726300009 ER PT J AU Cote, GL Skory, CD AF Cote, Gregory L. Skory, Christopher D. TI Effects of mutations at threonine-654 on the insoluble glucan synthesized by Leuconostoc mesenteroides NRRL B-1118 glucansucrase SO APPLIED MICROBIOLOGY AND BIOTECHNOLOGY LA English DT Article DE Glucansucrase; Dextransucrase; Dextran; Mutan; Gel; Insoluble polysaccharide ID GLUCOSYLTRANSFERASE GTF-I; STREPTOCOCCUS-MUTANS; ACCEPTOR REACTION; PRIMER DEXTRAN; POLYSACCHARIDE; MECHANISM; RESIDUES; SOBRINUS; STRAINS; PRODUCT AB Twelve different amino acids were each substituted for threonine-654 in a cloned glucansucrase from Leuconostoc mesenteroides NRRL B-1118. Both the native and the cloned enzyme with threonine at position 654 produced a water-insoluble glucan containing approximately 44 mol% 1,3-disubstituted alpha-d-glucopyranosyl units and 29 mol% 1,6-disubstituted alpha-d-glucopyranosyl units. Several substitutions yielded an enzyme that produced an increased percentage of 1,3-disubstituted alpha-d-glucopyranosyl units, with corresponding decreases in 1,6-disubstituted alpha-d-glucopyranosyl units. Only one substitution, tyrosine, resulted in a significant increase in the percentage of 1,6-disubstituted alpha-d-glucopyranosyl units, with a concomitant increase in glucan yield. The mutated enzymes that produced the highest levels of 1,3-disubstituted alpha-d-glucopyranosyl units were also significantly activated by the addition of dextran, but glucan yields were also lower in these mutants. C1 [Cote, Gregory L.; Skory, Christopher D.] ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Cote, GL (reprint author), ARS, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, USDA, 1815 N Univ St, Peoria, IL 61604 USA. EM greg.cote@ars.usda.gov NR 38 TC 9 Z9 9 U1 0 U2 11 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0175-7598 EI 1432-0614 J9 APPL MICROBIOL BIOT JI Appl. Microbiol. Biotechnol. PD AUG PY 2014 VL 98 IS 15 BP 6651 EP 6658 DI 10.1007/s00253-014-5622-x PG 8 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA AM5EY UT WOS:000339880300011 PM 24682484 ER PT J AU Lin, YH Abad, JA Maroon-Lango, CJ Perry, KL Pappu, HR AF Lin, Y. -H. Abad, J. A. Maroon-Lango, C. J. Perry, K. L. Pappu, H. R. TI Molecular characterization of domestic and exotic potato virus S isolates and a global analysis of genomic sequences SO ARCHIVES OF VIROLOGY LA English DT Article ID DNA POLYMORPHISM; RECOMBINANT SEQUENCES; MAXIMUM-LIKELIHOOD; STRAINS; IDENTIFICATION; VARIABILITY; DATAMONKEY; GENE AB Five potato virus S (PVS) isolates from the USA and three isolates from Chile were characterized based on biological and molecular properties to delineate these PVS isolates into either ordinary (PVSO) or Andean (PVSA) strains. Five isolates - 41956, Cosimar, Galaxy, ND2492-2R, and Q1 - were considered ordinary strains, as they induced local lesions on the inoculated leaves of Chenopodium quinoa, whereas the remaining three (FL206-1D, Q3, and Q5) failed to induce symptoms. Considerable variability of symptom expression and severity was observed among these isolates when tested on additional indicator plants and potato cv. Defender. Additionally, all eight isolates were characterized by determining the nucleotide sequences of their coat protein (CP) genes. Based on their biological and genetic properties, the 41956, Cosimar, Galaxy, ND2492-2R, and Q1 isolates were identified as PVSO. PVS-FL206-1D and the two Chilean isolates (PVS-Q3 and PVS-Q5) could not be identified based on phenotype alone; however, based on sequence comparisons, PVS-FL206-1D was identified as PVSO, while Q3 and Q5 clustered with known PVSA strains. C. quinoa may not be a reliable indicator for distinguishing PVS strains. Sequences of the CP gene should be used as an additional criterion for delineating PVS strains. A global genetic analysis of known PVS sequences from GenBank was carried out to investigate nucleotide substitution, population selection, and genetic recombination and to assess the genetic diversity and evolution of PVS. A higher degree of nucleotide diversity (pi value) of the CP gene compared to that of the 11K gene suggested greater variation in the CP gene. When comparing PVSA and PVSO strains, a higher pi value was found for PVSA. Statistical tests of the neutrality hypothesis indicated a negative selection pressure on both the CP and 11K proteins of PVSO, whereas a balancing selection pressure was found on PVSA. C1 [Lin, Y. -H.; Pappu, H. R.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Abad, J. A.; Maroon-Lango, C. J.] USDA, APHIS, PPQ, PHP,Plant Germplasm Quarantine Program, Beltsville, MD 20705 USA. [Perry, K. L.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. RP Pappu, HR (reprint author), Washington State Univ, Dept Plant Pathol, POB 646430, Pullman, WA 99164 USA. EM hrp@wsu.edu FU Washington State Potato Commission; Washington State Commission for Pesticide Registration, PPNS, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Washington State University, Pullman, WA, USA [0626, WNPO 0545] FX This study was supported in part by the Washington State Potato Commission and Washington State Commission for Pesticide Registration, PPNS No. 0626, Department of Plant Pathology, College of Agricultural, Human and Natural Resource Sciences, Agricultural Research Center, Project # WNPO 0545, Washington State University, Pullman, WA 99164-6430, USA. NR 39 TC 2 Z9 2 U1 2 U2 14 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD AUG PY 2014 VL 159 IS 8 BP 2115 EP 2122 DI 10.1007/s00705-014-2022-6 PG 8 WC Virology SC Virology GA AM5HQ UT WOS:000339888300026 PM 24610552 ER PT J AU Beniston, JW DuPont, ST Glover, JD Lal, R Dungait, JAJ AF Beniston, Joshua W. DuPont, S. Tianna Glover, Jerry D. Lal, Rattan Dungait, Jennifer A. J. TI Soil organic carbon dynamics 75 years after land-use change in perennial grassland and annual wheat agricultural systems SO BIOGEOCHEMISTRY LA English DT Article DE Soil organic carbon; Tallgrass prairie grassland; Deep-rooting perennial; Soil carbon turnover; Subsoil; Soil particle size fractionation ID C-13 NATURAL-ABUNDANCE; PARTICLE-SIZE FRACTIONS; SUBSOIL HORIZONS; STABILIZATION MECHANISMS; MATTER TURNOVER; DEEP SOIL; SEQUESTRATION; CULTIVATION; MANAGEMENT; FOREST AB The dynamics of roots and soil organic carbon (SOC) in deeper soil layers are amongst the least well understood components of the global C cycle, but essential if soil C is to be managed effectively. This study utilized a unique set of land-use pairings of harvested tallgrass prairie grasslands (C-4) and annual wheat croplands (C-3) that were under continuous management for 75 years to investigate and compare the storage, turnover and allocation of SOC in the two systems to 1 m depth. Cropland soils contained 25 % less SOC than grassland soils (115 and 153 Mg C ha(-1), respectively) to 1 m depth, and had lower SOC contents in all particle size fractions (2000-250, 250-53, 53-2 and < 2 mu m), which nominally correspond to SOC pools with different stability. Soil bulk delta C-13 values also indicated the significant turnover of grassland-derived SOC up to 80 cm depth in cropland soils in all fractions, including deeper (> 40 cm) layers and mineral-associated (< 53 mu m) SOC. Grassland soils had significantly more visible root biomass C than cropland soils (3.2 and 0.6 Mg ha(-1), respectively) and microbial biomass C (3.7 and 1.3 Mg ha(-1), respectively) up to 1 m depth. The outcomes of this study demonstrated that: (i) SOC pools that are perceived to be stable, i.e. subsoil and mineral-associated SOC, are affected by land-use change; and, (ii) managed perennial grasslands contained larger SOC stocks and exhibited much larger C allocations to root and microbial pools than annual croplands throughout the soil profile. C1 [Beniston, Joshua W.; Lal, Rattan] Ohio State Univ, Sch Environm & Nat Resources, Carbon Management & Sequestrat Ctr, Columbus, OH 43210 USA. [DuPont, S. Tianna] Penn State Univ, Nazareth, PA 18064 USA. [Glover, Jerry D.] US Agcy Int Dev, Bur Food Secur, Washington, DC 20523 USA. [Dungait, Jennifer A. J.] Rothamsted Res North Wyke, Dept Sustainable Syst & Grassland Sci, Okehampton EX20 2SB, Devon, England. RP Beniston, JW (reprint author), New Mexico State Univ, USDA, ARS, Las Cruces, NM 88003 USA. EM beniston@nmsu.edu RI Dungait, Jennifer/F-1110-2013 OI Dungait, Jennifer/0000-0001-9074-4174 FU Land Institute's graduate research program FX The authors wish to thank: John Mai for extensive field and laboratory work; Sandy Jones and David Ussiri for assistance with laboratory analyses; and Steve Culman, David W. Hopkins, and two anonymous reviewers for helpful reviews of earlier drafts of the manuscript. A portion of the funding for the study was provided by the The Land Institute's graduate research program. NR 73 TC 22 Z9 23 U1 8 U2 101 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD AUG PY 2014 VL 120 IS 1-3 BP 37 EP 49 DI 10.1007/s10533-014-9980-3 PG 13 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AM5BX UT WOS:000339871700003 ER PT J AU Toosi, ER Schmidt, JP Castellano, MJ AF Toosi, Ehsan R. Schmidt, John P. Castellano, Michael J. TI Land use and hydrologic flowpaths interact to affect dissolved organic matter and nitrate dynamics SO BIOGEOCHEMISTRY LA English DT Article DE Dissolved organic C (DOC); Dissolved organic N (DON); Land-use; Soil depth; Subsoil; Cultivation ID SOIL SOLUTION; PREFERENTIAL FLOW; FOREST SOIL; CARBON; NITROGEN; WATER; MECHANISMS; MANAGEMENT; RETENTION; TRANSPORT AB The transport and transformation of dissolved organic matter (DOM) and dissolved inorganic nitrogen (DIN) through the soil profile impact down-gradient ecosystems and are increasingly recognized as important factors affecting the balance between accumulation and mineralization of subsoil organic matter. Using zero tension and tension lysimeters at three soil depths (20, 40, 60 cm) in paired forest and maize/soybean land uses, we compared dissolved organic C (DOC), dissolved organic N (DON) and DIN concentrations as well as DOM properties including hydrophilic-C (HPI-C), UV absorption (SUVA(254)), humification index and C/N ratio. Soil moisture data collected at lysimeter locations suggest zero tension lysimeters sampled relatively rapid hydrologic flowpaths that included downward saturated flow through the soil matrix and/or rapid macropore flow that is not in equilibrium with bulk soil solution whereas tension lysimeters sampled relatively immobile soil matrix solution during unsaturated conditions. The effect of land use on DOC and DON concentrations was largely limited to the most shallow (20 cm) sampling depth where DOC concentrations were greater in the forest (only zero tension lysimeters) and DON concentrations were greater in the cropland (both lysimeter types). In contrast to DOC and DON concentrations, the effect of land use on DOM properties persisted to the deepest sampling depth (60 cm), suggesting that DOM in the cropland was more decomposed regardless of lysimeter type. DOC concentrations and DOM properties differed between lysimeter types only in the forest at 20 cm where soil solutions collected with zero tension lysimeters had greater DOC concentrations, greater SUVA(254), greater humification index and lower HPI-C. Our data highlight the importance of considering DOM quality in addition to DOC quantity, and indicate long-term cultivation reduced the delivery of relatively less decomposed DOM to all soil depths. C1 [Toosi, Ehsan R.; Castellano, Michael J.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Schmidt, John P.] ARS, Pasture Syst & Watershed Management Res Unit, USDA, University Pk, PA USA. RP Castellano, MJ (reprint author), Iowa State Univ, Dept Agron, Ames, IA 50011 USA. EM castellanomichaelj@gmail.com OI Castellano, Michael/0000-0003-1411-7931 FU USDA AFRI Award [20106510720445] FX We would like to thank Dr. A. Clapp and Dr. M. A. Tabatabai for providing analytical instrument access. We appreciate the assistance of Amanda Oswald, Michael Reiner, Sarah Fishel, David Otto, and Todd Strohecker. The research was funded by USDA AFRI Award #20106510720445. NR 42 TC 3 Z9 3 U1 8 U2 74 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD AUG PY 2014 VL 120 IS 1-3 BP 89 EP 104 DI 10.1007/s10533-014-9983-0 PG 16 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AM5BX UT WOS:000339871700006 ER PT J AU Smith, TE Kolka, RK Zhou, XB Helmers, MJ Cruse, RM Tomer, MD AF Smith, Tomorra E. Kolka, Randall K. Zhou, Xiaobo Helmers, Matthew J. Cruse, Richard M. Tomer, Mark D. TI Effects of native perennial vegetation buffer strips on dissolved organic carbon in surface runoff from an agricultural landscape SO BIOGEOCHEMISTRY LA English DT Article DE DOC; Prairie; Row-crop; Corn; Soybeans ID PRAIRIE FILTER STRIPS; LAND-USE; MANAGEMENT-PRACTICES; WATERSHEDS; EXPORT; STREAMS; NITROGEN; MATTER; DOC; USA AB Dissolved organic carbon (DOC) constitutes a small yet important part of a watershed's carbon budget because it is mobile and biologically active. Agricultural conservation practices such as native perennial vegetation (NPV) strips will influence carbon cycling of an upland agroecosystem, and could affect how much DOC enters streams in runoff, potentially affecting aquatic ecosystems. In a study conducted in Iowa (USA), four treatments with strips of NPV varying in slope position and proportion of area were randomly assigned among 12 small agricultural watersheds in a balanced incomplete block design. Runoff samples from 2008 to 2010 were analyzed for DOC and correlated with flow data to determine flow weighted DOC concentrations and loads. Data were analyzed for the entire 3 years, annually, seasonally, monthly, by flow event size and for one extreme storm event. Overall we found few differences in DOC concentration with the exception that concentrations were greater in the 10 % NPV at the footslope watersheds than the 20 % NPV in contours watersheds over the 3 years, and the 100 % agricultural treatment had higher DOC concentrations than all NPV treatments during the one extreme event. Because the NPV treatments reduced runoff, DOC export tended to be highest in the 100 % agricultural watersheds over the 3 years and during high flows. We also compared two watersheds that were restored to 100 % NPV and found decreases in DOC concentrations and loads indicating that complete conversion to prairie leads to less watershed DOC export. Regression results also support the contention that increases in the percentage of NPV in the watershed decreases watershed export of DOC. Further analysis indicated that DOC concentrations were diluted as flow event size increased, independent of any treatment effects. It appears groundwater sources become an important component to flow as flow event size increases in these watersheds. C1 [Smith, Tomorra E.; Zhou, Xiaobo; Helmers, Matthew J.; Cruse, Richard M.] Iowa State Univ, Ames, IA USA. [Kolka, Randall K.] US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. [Tomer, Mark D.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA USA. RP Kolka, RK (reprint author), US Forest Serv, USDA, No Res Stn, Grand Rapids, MN 55744 USA. EM rkolka@fs.fed.us NR 36 TC 1 Z9 1 U1 4 U2 38 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD AUG PY 2014 VL 120 IS 1-3 BP 121 EP 132 DI 10.1007/s10533-014-9985-y PG 12 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AM5BX UT WOS:000339871700008 ER PT J AU Hill, BH Elonen, CM Jicha, TM Kolka, RK Lehto, LLP Sebestyen, SD Seifert-Monson, LR AF Hill, Brian H. Elonen, Colleen M. Jicha, Terri M. Kolka, Randall K. Lehto, Larae L. P. Sebestyen, Stephen D. Seifert-Monson, Lindsey R. TI Ecoenzymatic stoichiometry and microbial processing of organic matter in northern bogs and fens reveals a common P-limitation between peatland types SO BIOGEOCHEMISTRY LA English DT Article DE Decomposition; Ecoenzymes; Peat; Soil; Stoichiometry ID CARBON-USE-EFFICIENCY; RIVERINE BACTERIOPLANKTON; THEORETICAL-MODEL; ENZYME-ACTIVITY; DECOMPOSITION; NITROGEN; SOIL; PHOSPHORUS; WETLANDS; GROWTH AB We compared carbon (C), nitrogen (N), and phosphorus (P) concentrations in atmospheric deposition, runoff, and soils with microbial respiration [dehydrogenase (DHA)] and ecoenzyme activity (EEA) in an ombrotrophic bog and a minerotrophic fen to investigate the environmental drivers of biogeochemical cycling in peatlands at the Marcell Experimental Forest in northern Minnesota, USA. Ecoenzymatic stoichiometry was used to construct models for C use efficiency (CUE) and decomposition (M), and these were used to model respiration (R-m). Our goals were to determine the relative C, N, and P limitations on microbial processes and organic matter decomposition, and to identify environmental constraints on ecoenzymatic processes. Mean annual water, C, and P yields were greater in the fen, while N yields were similar in both the bog and fen. Soil chemistry differed between the bog and fen, and both watersheds exhibited significant differences among soil horizons. DHA and EEA differed by watersheds and soil horizons, CUE, M, and R-m differed only by soil horizons. C, N, or P limitations indicated by EEA stoichiometry were confirmed with orthogonal regressions of ecoenzyme pairs and enzyme vector analyses, and indicated greater N and P limitation in the bog than in the fen, with an overall tendency toward P-limitation in both the bog and fen. Ecoenzymatic stoichiometry, microbial respiration, and organic matter decomposition were responsive to resource availability and the environmental drivers of microbial metabolism, including those related to global climate changes. C1 [Hill, Brian H.; Elonen, Colleen M.; Jicha, Terri M.; Lehto, Larae L. P.] US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Duluth, MN 55804 USA. [Kolka, Randall K.; Sebestyen, Stephen D.] US Forest Serv, Ctr Res Ecosyst Change, No Res Stn, Grand Rapids, MN 55744 USA. [Seifert-Monson, Lindsey R.] Univ Minnesota, Integrated Biosci Grad Program, Duluth, MN 55812 USA. RP Hill, BH (reprint author), US EPA, Midcontinent Ecol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, 6201 Congdon Blvd, Duluth, MN 55804 USA. EM hill.brian@epa.gov RI Sebestyen, Stephen/D-1238-2013 OI Sebestyen, Stephen/0000-0002-6315-0108 NR 60 TC 16 Z9 16 U1 15 U2 111 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD AUG PY 2014 VL 120 IS 1-3 BP 203 EP 224 DI 10.1007/s10533-014-9991-0 PG 22 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AM5BX UT WOS:000339871700013 ER PT J AU Brahney, J Ballantyne, AP Kociolek, P Spaulding, S Otu, M Porwoll, T Neff, JC AF Brahney, J. Ballantyne, A. P. Kociolek, P. Spaulding, S. Otu, M. Porwoll, T. Neff, J. C. TI Dust mediated transfer of phosphorus to alpine lake ecosystems of the Wind River Range, Wyoming, USA SO BIOGEOCHEMISTRY LA English DT Article DE Atmospheric deposition; Dust; Phosphorus; Alpine lakes; Wyoming ID MOUNTAIN LAKES; SIERRA-NEVADA; CATCHMENT CHARACTERISTICS; ATMOSPHERIC DEPOSITION; RESOURCE REQUIREMENTS; EOLIAN SEDIMENTATION; DIATOM ASSEMBLAGES; INFERENCE MODELS; WATER CHEMISTRY; SOILS AB Alpine lakes receive a large fraction of their nutrients from atmospheric sources and are consequently sensitive to variations in both the amount and chemistry of atmospheric deposition. In this study we explored the spatial changes in lake water chemistry and biology along a gradient of dust deposition in the Wind River Range, Wyoming. Regional differences were explored using the variation in bulk deposition, lake water, sediment, and bedrock geochemistry and catchment characteristics. Dust deposition rates in the Southwestern region averaged 3.34 g m(-2) year(-1), approximately three times higher than deposition rates in the Northwestern region (average 1.06 g m(-2) year(-1)). Dust-P deposition rates ranged from 87 A mu g P m(2) day(-1) in the Northwestern region to 276 A mu g P m(2) day(-1) in the Southwestern region. Subalpine and alpine lakes in the Southwestern region had greater total phosphorus (TP) concentrations (5-13 A mu g L-1) and greater sediment phosphorus (SP) concentrations (2-5 mg g(-1)) than similar lakes elsewhere in the region (1-8 A mu g L-1 TP, 0.5-2 mg g(-1) SP). Lake phosphorus concentrations were related to dissolved organic carbon (DOC) across vegetation gradients, but related to the percent of bare rock, catchment area to lake area, and catchment steepness across dust deposition gradients. Modern phytoplankton and zooplankton biomasses were two orders of magnitude greater in the Southwest than in the Northwest, and alpine lakes in the Southwest had a unique diatom species assemblage with relatively higher concentrations of Asterionella formosa, Pseudostaurosira pseudoconstruens, and Pseudostaurosira brevistriata. These results suggests that catchment controls on P export to lakes (i.e. DOC) are overridden in dominantly bare rock basins where poor soils cannot effectively retain dust deposited P. C1 [Brahney, J.; Neff, J. C.] Univ Colorado, Dept Geol Sci, Boulder, CO 80309 USA. [Ballantyne, A. P.] Univ Montana, Dept Ecosyst & Conservat Sci, Missoula, MT 59812 USA. [Kociolek, P.] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA. [Spaulding, S.; Otu, M.] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. [Porwoll, T.] US Forest Serv, Bridger Teton Natl Forest, Pinedale, WY USA. RP Brahney, J (reprint author), Univ British Columbia, Dept Earth & Environm Sci, 3333 Univ Way, Kelowna, BC V1V 1V7, Canada. EM jbrahney@gmail.com OI NEFF, JASON/0000-0002-8290-1472; Brahney, Janice/0000-0001-7614-2855 FU NSF DEB Award [0948823]; Niwot Ridge LTER Site (NSF) [1027341]; Colorado Mountain Club; John W. Marr Ecology Fund; Shell; Climate and Land Use Program at the USGS; NSERC (PGSD) FX Support was provided by NSF DEB Award #0948823, Niwot Ridge LTER Site (NSF #1027341), Colorado Mountain Club, John W. Marr Ecology Fund, Shell, Climate and Land Use Program at the USGS, and NSERC (PGSD). We thank J. Grenon, R. Propri, T. Jones, H. Gowans, C. Flag, G. Farlow, T. Conquest, C. Blum, and B. Thompson for field assistance and A. Heathcoat for valuable discussion on the statistical methods. We also thank the anonymous reviewers and J. Sickman for valuable commentary on the manuscript. NR 73 TC 8 Z9 8 U1 5 U2 36 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0168-2563 EI 1573-515X J9 BIOGEOCHEMISTRY JI Biogeochemistry PD AUG PY 2014 VL 120 IS 1-3 BP 259 EP 278 DI 10.1007/s10533-014-9994-x PG 20 WC Environmental Sciences; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Geology GA AM5BX UT WOS:000339871700016 ER PT J AU Foti, R Ramirez, JA Brown, TC AF Foti, Romano Ramirez, Jorge A. Brown, Thomas C. TI A probabilistic framework for assessing vulnerability to climate variability and change: the case of the US water supply system SO CLIMATIC CHANGE LA English DT Article ID COLORADO RIVER-BASIN; UNITED-STATES; RESOURCES; HYDROLOGY; WITHDRAWALS; IMPACTS AB We introduce a probabilistic framework for vulnerability analysis and use it to quantify current and future vulnerability of the US water supply system. We also determine the contributions of hydro-climatic and socio-economic drivers to the changes in projected vulnerability. For all scenarios and global climate models examined, the US Southwest including California and the southern Great Plains was consistently found to be the most vulnerable. For most of the US, the largest contributions to changes in vulnerability come from changes in supply. However, for some areas of the West changes in vulnerability are caused mainly by changes in demand. These changes in supply and demand result mainly from changes in evapotranspiration rather than from changes in precipitation. Importantly, changes in vulnerability from projected changes in the standard deviations of precipitation and evapotranspiration are of about the same magnitude or larger than those from changes in the corresponding means over most of the US, except in large areas of the Great Plains, in central California and southern and central Texas. C1 [Foti, Romano; Ramirez, Jorge A.; Brown, Thomas C.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Brown, Thomas C.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. RP Ramirez, JA (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. EM Jorge.Ramirez@ColoState.edu FU U.S. Forest Service; U.S. Bureau of Reclamation FX This study was performed in response to the Forest and Rangeland Renewable Resources Planning Act of 1974 (public law 93-378). Funding was provided by the U.S. Forest Service with substantial contributions from Colorado State University. Partial funding was provided by the U.S. Bureau of Reclamation. NR 28 TC 2 Z9 2 U1 3 U2 43 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD AUG PY 2014 VL 125 IS 3-4 BP 413 EP 427 DI 10.1007/s10584-014-1111-6 PG 15 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AM6JM UT WOS:000339970700010 ER PT J AU Foti, R Ramirez, JA Brown, TC AF Foti, Romano Ramirez, Jorge A. Brown, Thomas C. TI Response surfaces of vulnerability to climate change: the Colorado River Basin, the High Plains, and California SO CLIMATIC CHANGE LA English DT Article ID WATER-RESOURCES; VEGETATION; SOIL; HYDROLOGY; IMPACTS; US; DYNAMICS; BALANCE; MODEL AB We quantify the vulnerability of water supply to shortage for the Colorado River Basin and basins of the High Plains and California and assess the sensitivity of their water supply system to future changes in the statistical variability of supply and demand. We do so for current conditions and future socio-economic scenarios within a probabilistic framework that incorporates the inherent uncertainties in the drivers of vulnerability. Our analysis indicates that the most sensitive basins to both current and future variability of demand and supply are the Central California and the San Joaquin-Tulare basins. Large sensitivity is also found for the Kansas basin of the High Plains. Within the Colorado River Basin, the Lower Colorado and Gila were found to be the most vulnerable and sensitive sub-basins. By accounting for future uncertainty within the above probabilistic framework, this study unveils and isolates the individual responses of a given basin to changes in the statistical properties of demand and supply and offers a valuable tool for the identification of policy strategies and adaptation measures. C1 [Foti, Romano; Ramirez, Jorge A.; Brown, Thomas C.] Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. [Brown, Thomas C.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. RP Ramirez, JA (reprint author), Colorado State Univ, Dept Civil & Environm Engn, Ft Collins, CO 80523 USA. EM Jorge.Ramirez@ColoState.edu FU U.S. Forest Service; U.S. Bureau of Reclamation FX This study was performed in response to the Forest and Rangeland Renewable Resources Planning Act of 1974 (public law 93-378). Funding was provided by the U.S. Forest Service with substantial contributions from Colorado State University. Partial funding was provided by the U.S. Bureau of Reclamation. NR 47 TC 0 Z9 0 U1 3 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0165-0009 EI 1573-1480 J9 CLIMATIC CHANGE JI Clim. Change PD AUG PY 2014 VL 125 IS 3-4 BP 429 EP 444 DI 10.1007/s10584-014-1178-0 PG 16 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AM6JM UT WOS:000339970700011 ER PT J AU Roffler, GH Talbot, SL Luikart, G Sage, GK Pilgrim, KL Adams, LG Schwartz, MK AF Roffler, Gretchen H. Talbot, Sandra L. Luikart, Gordon Sage, George K. Pilgrim, Kristy L. Adams, Layne G. Schwartz, Michael K. TI Lack of sex-biased dispersal promotes fine-scale genetic structure in alpine ungulates SO CONSERVATION GENETICS LA English DT Article DE Fine-scale genetic structure; Sex-biased dispersal; Philopatry; Landscape genetics; Population connectivity; Non-invasive sampling; Ovis dalli dalli ID MITOCHONDRIAL-DNA VARIATION; DESERT BIGHORN SHEEP; POPULATION-STRUCTURE; LANDSCAPE GENETICS; MULTILOCUS GENOTYPES; STATISTICAL-MODEL; CHAMOIS RUPICAPRA; MOUNTAIN SHEEP; MATING SYSTEMS; FLOW AB Identifying patterns of fine-scale genetic structure in natural populations can advance understanding of critical ecological processes such as dispersal and gene flow across heterogeneous landscapes. Alpine ungulates generally exhibit high levels of genetic structure due to female philopatry and patchy configuration of mountain habitats. We assessed the spatial scale of genetic structure and the amount of gene flow in 301 Dall's sheep (Ovis dalli dalli) at the landscape level using 15 nuclear microsatellites and 473 base pairs of the mitochondrial (mtDNA) control region. Dall's sheep exhibited significant genetic structure within contiguous mountain ranges, but mtDNA structure occurred at a broader geographic scale than nuclear DNA within the study area, and mtDNA structure for other North American mountain sheep populations. No evidence of male-mediated gene flow or greater philopatry of females was observed; there was little difference between markers with different modes of inheritance (pairwise nuclear DNA F (ST) = 0.004-0.325; mtDNA F (ST) = 0.009-0.544), and males were no more likely than females to be recent immigrants. Historical patterns based on mtDNA indicate separate northern and southern lineages and a pattern of expansion following regional glacial retreat. Boundaries of genetic clusters aligned geographically with prominent mountain ranges, icefields, and major river valleys based on Bayesian and hierarchical modeling of microsatellite and mtDNA data. Our results suggest that fine-scale genetic structure in Dall's sheep is influenced by limited dispersal, and structure may be weaker in populations occurring near ancestral levels of density and distribution in continuous habitats compared to other alpine ungulates that have experienced declines and marked habitat fragmentation. C1 [Roffler, Gretchen H.; Talbot, Sandra L.; Sage, George K.; Adams, Layne G.] US Geol Survey, Alaska Sci Ctr, Anchorage, AK 99508 USA. [Luikart, Gordon] Univ Montana, Flathead Lake Biol Stn, Fish & Wildlife Genom Grp, Div Biol Sci, Polson, MT 59860 USA. [Pilgrim, Kristy L.; Schwartz, Michael K.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Roffler, GH (reprint author), US Geol Survey, Alaska Sci Ctr, 4210 Univ Dr,Suite 201, Anchorage, AK 99508 USA. EM groffler@usgs.gov RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU National Park Service; U. S. Geological Survey; Murie Science and Learning Center; NSF-DEB [1067613, 0742181] FX This work was supported by funding from the National Park Service, the U. S. Geological Survey, and the Murie Science and Learning Center. For assistance with fieldwork, we thank T. Cambier, H. McMahan, J. Putera, M. Reid, and R. Schwanke. We are grateful for the help of T. Bentzen, N. Cassara, T. Kavalok, T. Fauline, T. Peltier, R. Schwanke, C. Schwanke, R. Stadtmiller, and R. Tobey in collecting muscle samples. K. Scribner, E. Rominger, and J. Wehausen, provided constructive criticism of the study plan, and F. W. Allendorf, M. Hebblewhite, J. Pearce, and R. Wilson of the manuscript drafts. GL was supported by NSF-DEB grants 1067613 and 0742181. Use of trade names does not imply endorsement by the U. S. Government. NR 98 TC 4 Z9 4 U1 7 U2 48 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD AUG PY 2014 VL 15 IS 4 BP 837 EP 851 DI 10.1007/s10592-014-0583-2 PG 15 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA AM3DQ UT WOS:000339732800008 ER PT J AU Landguth, EL Schwartz, MK AF Landguth, Erin L. Schwartz, Michael K. TI Evaluating sample allocation and effort in detecting population differentiation for discrete and continuously distributed individuals SO CONSERVATION GENETICS LA English DT Article DE CDPOP; F-ST; Isolation-by-distance; Isolation-by-barrier; Sampling optimization; Simulation modeling ID LANDSCAPE RESISTANCE; GENETIC-STRUCTURE; DISTANCE; MARKERS; MICROSATELLITES; SIMULATIONS; DISPERSAL; PROGRAM; FLOW AB One of the most pressing issues in spatial genetics concerns sampling. Traditionally, substructure and gene flow are estimated for individuals sampled within discrete populations. Because many species may be continuously distributed across a landscape without discrete boundaries, understanding sampling issues becomes paramount. Given large-scale, geographically broad conservation efforts, researchers are looking for guidance as to the trade-offs between sampling more individuals within a population versus few individuals scattered across more populations. Here, we conducted simulations that address these issues. We first established two archetypical patterns of dispersion: (1) individuals within discrete populations, and (2) continuously distributed individuals with limited dispersal. We used genotypes generated from a spatially-explicit, individual-based program and simulated genetic structure in individuals from nine different population sizes across a landscape that either had barriers to movement (defining discrete populations) or isolation-by-distance patterns (defining continuously distributed individuals). Then, given each pattern of dispersion, we allocated samples across four different sampling strategies for each of the nine population sizes in various configurations for sampling more individuals within a population versus fewer individuals scattered across more populations. We assessed the population genetic substructure with both the population-based metric, F (ST), and an individual-based metric, D (PS) regardless of the true pattern of dispersion to allow us to better understand the effect of incorrectly matching the metric and the distribution (e.g., F (ST) with continuously distributed individuals, and vice versa). We show that sampling many subpopulations (or sampling areas), thus sampling fewer individuals per subpopulation, overestimates measures of population subdivision with the population-based metric for both patterns of dispersion. In contrast, using the individual-based metric gives the opposite results: sampling too few subpopulations, and many individuals per subpopulation, produces an underestimate of the strength of isolation-by-distance. By comparing all results, we were able to suggest a strong predictive model of a chosen genetic structure metric for elucidating the sampling design trade-offs given each pattern of dispersion and configuration on the landscape. C1 [Landguth, Erin L.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. RP Landguth, EL (reprint author), Univ Montana, Div Biol Sci, 32 Campus Dr, Missoula, MT 59812 USA. EM erin.landguth@mso.umt.edu RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 NR 36 TC 8 Z9 8 U1 5 U2 34 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1566-0621 EI 1572-9737 J9 CONSERV GENET JI Conserv. Genet. PD AUG PY 2014 VL 15 IS 4 BP 981 EP 992 DI 10.1007/s10592-014-0593-0 PG 12 WC Biodiversity Conservation; Genetics & Heredity SC Biodiversity & Conservation; Genetics & Heredity GA AM3DQ UT WOS:000339732800018 ER EF