FN Thomson Reuters Web of Science™ VR 1.0 PT J AU Dong, DS Davis, CG Stewart, H AF Dong, Diansheng Davis, Christopher G. Stewart, Hayden TI The quantity and variety of households' meat purchases: A censored demand system approach SO AGRICULTURAL ECONOMICS LA English DT Article DE C34; C51; D12; Demand elasticity; Food variety; Household meat purchase; Censored demand system; AIDS model; Maximum likelihood; Simulated probability ID STRUCTURAL-CHANGE; CONSUMER DEMAND; UNIT VALUE; QUALITY; BEEF; PRODUCTS; DYNAMICS; MODELS; PRICE; FOOD AB This study investigates the demand for 10 disaggregated meat products by U.S. households. A censored demand system which imposes budget constraints in both observed and latent shares is estimated along with a detailed explanation of the estimation procedure. Additional details about the methodology not provided in previous studies should help researchers to empirically apply the model. An innovation of the study is to introduce the censored demand system model for studying the variety of a household's food purchases. Most households buy between seven and eight different products and hardly change the variety of their purchases with changes in meat expenditure. C1 [Dong, Diansheng; Davis, Christopher G.; Stewart, Hayden] ERS USDA, Washington, DC 20024 USA. RP Dong, DS (reprint author), ERS USDA, 355 E St SW, Washington, DC 20024 USA. EM ddong@ers.usda.gov NR 36 TC 0 Z9 0 U1 1 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0169-5150 EI 1574-0862 J9 AGR ECON-BLACKWELL JI Agric. Econ. PD JAN PY 2015 VL 46 IS 1 BP 99 EP 112 DI 10.1111/agec.12143 PG 14 WC Agricultural Economics & Policy; Economics SC Agriculture; Business & Economics GA AY1DO UT WOS:000347334000008 ER PT J AU Huff, LM Potts, DL Hamerlynck, EP AF Huff, L. M. Potts, D. L. Hamerlynck, E. P. TI Ecosystem CO2 Exchange in Response to Nitrogen and Phosphorus Addition in a Restored, Temperate Grassland SO AMERICAN MIDLAND NATURALIST LA English DT Article ID CARBON-DIOXIDE EXCHANGE; PRIMARY PRODUCTIVITY; SOIL-MOISTURE; GLOBAL CHANGE; PLANT; LIMITATION; DEPOSITION; WATER; COMMUNITIES; CALIFORNIA AB Nitrogen (N) and phosphorus (P) affect the structure and function of grasslands by altering plant competitive interactions, shifting patterns of above and below ground biomass allocation, and increasing net primary production. However, the influence of N and P on net ecosystem CO2 exchange (NEE) is poorly understood. In a field-based factorial N- and P-addition experiment, we measured shallow soil moisture, leaf area index, and component fluxes of midday ecosystem CO2 exchange throughout the growing season in a restored temperate grassland near Buffalo, New York. Throughout the growing season, N-addition increased gross ecosystem CO2 exchange (GEE) and correspondingly altered NEE to increase ecosystem CO2 uptake. In contrast N-addition caused a seasonally dynamic decline in leaf area adjusted GEE, a pattern consistent with increased photosynthetic light limitation. P-addition did not significantly increase Re, and N- and P-addition interacted to significantly weaken the ecosystem as a midday CO2 sink. Moreover, water limitation and phenological constraints during the middle and late growing season appear to limit plant responses to nutrient addition. These results suggest influences of N -and P-addition on ecosystem processes are seasonally dynamic and by differentially influencing above and below ground components of ecosystems, the availability of N and P in soils may interact to influence ecosystem CO2 exchange. C1 [Huff, L. M.; Potts, D. L.] SUNY Coll Buffalo, Buffalo, NY 14222 USA. [Hamerlynck, E. P.] USDA ARS, Eastern Oregon Agr Res Ctr, Burns, OR 97720 USA. RP Potts, DL (reprint author), SUNY Coll Buffalo, Biol Dept SAMC 213, Buffalo, NY 14222 USA. EM pottsdl@buffalostate.edu FU Buffalo State Research Foundation; Buffalo State School of Natural and Social Sciences; Buffalo State Community Partnerships Office FX D. Huff, M. Socha, J. Hirtreiter, C. Holmgren, J. Louie, E. Snyder, N. Wood and J. Wuerstle assisted in the field and in the laboratory. We thank the Buffalo Museum of Science for providing access Tifft Nature Preserve as well as the generous cooperation and support of D. Spiering, managing ecologist of Tifft Nature Preserve. C. Pennuto and E. Standora provided thoughtful comments on an earlier draft of this manuscript. Financial support was provided by the Buffalo State Research Foundation, the Buffalo State School of Natural and Social Sciences and by a grant from the Buffalo State Community Partnerships Office. NR 58 TC 1 Z9 2 U1 3 U2 52 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 EI 1938-4238 J9 AM MIDL NAT JI Am. Midl. Nat. PD JAN PY 2015 VL 173 IS 1 BP 73 EP 87 DI 10.1674/0003-0031-173.1.73 PG 15 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX9ST UT WOS:000347243100007 ER PT J AU Keller, BJ Bleisch, AD Millspaugh, JJ Lehman, CP Kragel, JJ Hansen, LP Sumners, J Rumble, MA Brundige, GC AF Keller, Barbara J. Bleisch, Amy D. Millspaugh, Joshua J. Lehman, Chad P. Kragel, Jackie J. Hansen, Lonnie P. Sumners, Jason Rumble, Mark A. Brundige, Gary C. TI Extended Duration of Parturition Season in North American Elk (Cervus elaphus) SO AMERICAN MIDLAND NATURALIST LA English DT Article ID WHITE-TAILED DEER; FACTORS AFFECTING REPRODUCTION; FEMALE RED DEER; ODOCOILEUS-VIRGINIANUS; CONCEPTION; PREGNANCY; ACCURACY; MONTANA; AGE AB The timing of births in ungulates has significant implications for juvenile survival and population growth. For North American elk (Cervus elaphus), typical parturition season ranges from late May to early Jun., and juveniles born outside of this peak characteristically exhibit lowered survival. We observed abnormally long parturition seasons in free-ranging elk populations in Missouri and South Dakota during 2012. Both populations exhibited late births; the last known births occurred on 26 Sep. in Missouri and 4 Sep. in South Dakota. Duration of parturition season was 112 and 119 d in Missouri and South Dakota, respectively. In Missouri, late births likely resulted from breeding by both yearling females and males. Late parturition in South Dakota may be caused by extended estrous cycles of elk that occurred on high quality range where few adult males were located. C1 [Keller, Barbara J.; Bleisch, Amy D.; Millspaugh, Joshua J.] Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO USA. [Lehman, Chad P.; Kragel, Jackie J.] South Dakota Dept Game Fish & Pk, Custer, SD 57730 USA. [Hansen, Lonnie P.; Sumners, Jason] Missouri Dept Conservat, Columbia, MO 65201 USA. [Rumble, Mark A.] US Forest Serv, USDA, Rocky Mt Res Stn, Rapid City, SD 57702 USA. [Brundige, Gary C.] Custer State Pk, Custer, SD 57730 USA. RP Keller, BJ (reprint author), Univ Missouri, Dept Fisheries & Wildlife Sci, Columbia, MO USA. EM KellerBJ@missouri.edu FU Rocky Mountain Elk Foundation; University of Missouri; Missouri Department of Conservation; South Dakota Department of Game, Fish and Parks FX Funding for this research was provided by the Rocky Mountain Elk Foundation, the University of Missouri, the Missouri Department of Conservation, and the South Dakota Department of Game, Fish and Parks. Field support in Missouri was provided by R. Houf, P. Mabry, M. Price, P. Vessels, S. Snow, T. Wolf, T. Schrautemeier, J. Ashling, D. Neel, J. Foggia, and S. Raiman. Field support in South Dakota was provided by D. Fogarty, A. Coleman, K. Kaskie, N. Markl, L. Schmitz, T. Gingery, M. Lenny, G. Lenny, L. Flack, and K. Knudsen. Additional field assistance and technical support was provided by The United States Forest Service Rocky Mountain Research Station. All handling, marking, and monitoring procedures were approved by the South Dakota State University Research Committee (Animal Care and Use Committee Approval Number 11-012A) and the University of Missouri Research Committee (Animal Care and Use Committee Approval Number 6909). NR 28 TC 0 Z9 0 U1 1 U2 13 PU AMER MIDLAND NATURALIST PI NOTRE DAME PA UNIV NOTRE DAME, BOX 369, ROOM 295 GLSC, NOTRE DAME, IN 46556 USA SN 0003-0031 EI 1938-4238 J9 AM MIDL NAT JI Am. Midl. Nat. PD JAN PY 2015 VL 173 IS 1 BP 162 EP 167 DI 10.1674/0003-0031-173.1.162 PG 6 WC Biodiversity Conservation; Ecology SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX9ST UT WOS:000347243100015 ER PT J AU Nirmala, X Schetelig, MF Zimowska, GJ Zhou, L Handler, AM AF Nirmala, Xavier Schetelig, Marc F. Zimowska, Grazyna J. Zhou, Lei Handler, Alfred M. TI Pro-apoptotic gene regulation and its activation by gamma-irradiation in the Caribbean fruit fly, Anastrepha suspensa SO APOPTOSIS LA English DT Article DE Ashid; Asrpr; Irradiation; Ecdysteroids; Cell death; Tephritids ID PROGRAMMED CELL-DEATH; DROSOPHILA EMBRYOS; DNA-DAMAGE; REAPER; HID; METAMORPHOSIS; EXPRESSION; EVOLUTION; HIERARCHY; PROTEIN AB Transcriptional activation of pro-apoptotic genes in response to cytotoxic stimuli is a conserved feature of the cell death pathway in metazoans. However, understanding the extent of this conservation in insects has been limited by the lack of known pro-apoptotic genes in non-drosophilids. Recently, we described the pro-apoptotic genes, Asrpr and Ashid, from the tephritid, Anastrepha suspensa, that now allow us to explore the conservation of pro-apoptotic gene regulation between a tephritid and drosophilids. In this study, we determined the developmental profiles of Asrpr and Ashid transcripts during embryogenesis and in embryos exposed to gamma-irradiation. Transcript levels of both genes determined by qRT-PCR were low throughout embryogenesis, with strong Ashid expression occurring during early to mid-embryogenesis and Asrpr expression peaking in late embryogenesis. This correlated to acridine orange stained apoptotic cells first appearing at 17 h and increasing over time. However, when irradiated at 16 h post-oviposition embryos exhibited significant levels of apoptosis consistent with strong induction of Asrpr and Ashid transcript levels by gamma-irradiation in young embryos < 24 h post-oviposition. Furthermore, embryos irradiated < 24 h post-oviposition failed to hatch, those irradiated between 24 and 32 h had increased hatching rates, but between 48 and 72 h irradiation had no effect on egg hatching. This indicates a transition of embryos from an irradiation-sensitive to irradiation-resistance stage between 24 and 48 h. Throughout post-embryonic development, the two pro-apoptotic genes share similar patterns of up-regulated gene expression, which correlate to ecdysone-induced developmental events, especially during metamorphosis. Together these results provide the first direct evidence for a conserved molecular mechanism of the programmed cell death pathway in insects. C1 [Nirmala, Xavier; Schetelig, Marc F.; Zimowska, Grazyna J.; Handler, Alfred M.] ARS, USDA, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Nirmala, Xavier] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Schetelig, Marc F.] Univ Giessen, D-35392 Giessen, Germany. [Zhou, Lei] Univ Florida, Coll Med, Dept Mol Genet & Microbiol, Gainesville, FL 32611 USA. [Zhou, Lei] Univ Florida, Coll Med, UF Shands Canc Ctr, Gainesville, FL 32611 USA. RP Nirmala, X (reprint author), ARS, USDA, Ctr Med Agr & Vet Entomol, 1700 SW 23rd Dr, Gainesville, FL 32608 USA. EM xnirmala@yahoo.com FU USDA-NIFA-Agriculture and Food Research Initiative; German Research Foundation [2011-39211-30769, SCHE 1833/1]; LOEWE Center for Insect Biotechnology Bioresources FX We thank Dr. Chris Geden for assistance with irradiation protocols. Funding from the USDA-NIFA-Agriculture and Food Research Initiative and the Biotechnology Risk Assessment Program (Grant #2011-39211-30769) (AMH), Emmy Noether program SCHE 1833/1 of the German Research Foundation (MFS), and the LOEWE Center for Insect Biotechnology & Bioresources (MFS) are gratefully acknowledged. NR 25 TC 2 Z9 2 U1 1 U2 13 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1360-8185 EI 1573-675X J9 APOPTOSIS JI Apoptosis PD JAN PY 2015 VL 20 IS 1 BP 1 EP 9 DI 10.1007/s10495-014-1055-3 PG 9 WC Biochemistry & Molecular Biology; Cell Biology SC Biochemistry & Molecular Biology; Cell Biology GA AY4DR UT WOS:000347528900001 PM 25433919 ER PT J AU Hildebrand, A Szewczyk, E Lin, H Kasuga, T Fan, ZL AF Hildebrand, Amanda Szewczyk, Edyta Lin, Hui Kasuga, Takao Fan, Zhiliang TI Engineering Neurospora crassa for Improved Cellobiose and Cellobionate Production SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID CARBON CATABOLITE REPRESSION; BETA-RECOMBINASE/SIX CASSETTE; ASPERGILLUS-NIDULANS; TRICHODERMA-REESEI; CELLOBIOHYDROLASE I; HYPOCREA-JECORINA; ETHANOL REGULON; BINDING-SITES; GENE DELETION; FUEL ETHANOL AB We report engineering Neurospora crassa to improve the yield of cellobiose and cellobionate from cellulose. A previously engineered strain of N. crassa (F5) with six of seven beta-glucosidase (bgl) genes knocked out was shown to produce cellobiose and cellobionate directly from cellulose without the addition of exogenous cellulases. In this study, the F5 strain was further modified to improve the yield of cellobiose and cellobionate from cellulose by increasing cellulase production and decreasing product consumption. The effects of two catabolite repression genes, cre-1 and ace-1, on cellulase production were investigated. The F5 Delta ace-1 mutant showed no improvement over the wild type. The F5 Delta cre-1 and F5 Delta ace-1 Delta cre-1 strains showed improved cellobiose dehydrogenase and exoglucanase expression. However, this improvement in cellulase expression did not lead to an improvement in cellobiose or cellobionate production. The cellobionate phosphorylase gene (ndvB) was deleted from the genome of F5 Delta ace-1 Delta cre-1 to prevent the consumption of cellobiose and cellobionate. Despite a slightly reduced hydrolysis rate, the F5 Delta ace-1 Delta cre-1 Delta ndvB strain converted 75% of the cellulose consumed to the desired products, cellobiose and cellobionate, compared to 18% converted by the strain F5 Delta ace-1 Delta cre-1. C1 [Hildebrand, Amanda; Szewczyk, Edyta; Lin, Hui; Fan, Zhiliang] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. [Kasuga, Takao] Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA. [Kasuga, Takao] USDA ARS, Davis, CA USA. RP Fan, ZL (reprint author), Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. EM jzfan@ucdavis.edu FU Agriculture and Food Research Initiative Competitive from the USDA National Institute of Food and Agriculture [2011-67009-20060]; EPA Star fellowship; Cota-Robles fellowship FX This project was supported by Agriculture and Food Research Initiative Competitive Grant no. 2011-67009-20060 from the USDA National Institute of Food and Agriculture and by an EPA Star fellowship and Cota-Robles fellowship awarded to Amanda Hildebrand. NR 46 TC 9 Z9 9 U1 5 U2 27 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JAN PY 2015 VL 81 IS 2 BP 597 EP 603 DI 10.1128/AEM.02885-14 PG 7 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AY1UL UT WOS:000347377500015 PM 25381238 ER PT J AU Wang, Z Leary, DH Malanoski, AP Li, RW Hervey, WJ Eddie, BJ Tender, GS Yanosky, SG Vora, GJ Tender, LM Lin, B Strycharz-Glaven, SM AF Wang, Zheng Leary, Dagmar H. Malanoski, Anthony P. Li, Robert W. Hervey, W. Judson Eddie, Brian J. Tender, Gabrielle S. Yanosky, Shelley G. Vora, Gary J. Tender, Leonard M. Lin, Baochuan Strycharz-Glaven, Sarah M. TI A Previously Uncharacterized, Nonphotosynthetic Member of the Chromatiaceae Is the Primary CO2-Fixing Constituent in a Self-Regenerating Biocathode SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID ELECTRON-TRANSFER; MICROBIAL ELECTROSYNTHESIS; GEOBACTER-SULFURREDUCENS; METAPROTEOMIC ANALYSES; COMMODITY CHEMICALS; MASS-SPECTROMETRY; GENE-EXPRESSION; BACTERIA; PROTEIN; BIOFILMS AB Biocathode extracellular electron transfer (EET) may be exploited for biotechnology applications, including microbially mediated O-2 reduction in microbial fuel cells and microbial electrosynthesis. However, biocathode mechanistic studies needed to improve or engineer functionality have been limited to a few select species that form sparse, homogeneous biofilms characterized by little or no growth. Attempts to cultivate isolates from biocathode environmental enrichments often fail due to a lack of some advantage provided by life in a consortium, highlighting the need to study and understand biocathode consortia in situ. Here, we present metagenomic and metaproteomic characterization of a previously described biocathode biofilm (+310 mV versus a standard hydrogen electrode [SHE]) enriched from seawater, reducing O-2, and presumably fixing CO2 for biomass generation. Metagenomics identified 16 distinct cluster genomes, 15 of which could be assigned at the family or genus level and whose abundance was roughly divided between Alpha- and Gammaproteobacteria. A total of 644 proteins were identified from shotgun metaproteomics and have been deposited in the the ProteomeXchange with identifier PXD001045. Cluster genomes were used to assign the taxonomic identities of 599 proteins, with Marinobacter, Chromatiaceae, and Labrenzia the most represented. RubisCO and phosphoribulokinase, along with 9 other Calvin-Benson-Bassham cycle proteins, were identified from Chromatiaceae. In addition, proteins similar to those predicted for iron oxidation pathways of known iron-oxidizing bacteria were observed for Chromatiaceae. These findings represent the first description of putative EET and CO2 fixation mechanisms for a self-regenerating, self-sustaining multispecies biocathode, providing potential targets for functional engineering, as well as new insights into biocathode EET pathways using proteomics. C1 [Wang, Zheng; Leary, Dagmar H.; Malanoski, Anthony P.; Hervey, W. Judson; Vora, Gary J.; Tender, Leonard M.; Lin, Baochuan; Strycharz-Glaven, Sarah M.] Naval Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA. [Li, Robert W.] ARS, USDA, Anim Genom & Improvement Lab, Beltsville, MD USA. [Eddie, Brian J.; Tender, Gabrielle S.] CALTECH, Pasadena, CA 91125 USA. [Yanosky, Shelley G.] Amer Soc Engn Educ, Washington, DC USA. RP Strycharz-Glaven, SM (reprint author), Naval Res Lab, Ctr Bio Mol Sci & Engn, Washington, DC 20375 USA. EM sarah.glaven@nrl.navy.mil RI Lin, Baochuan/A-8390-2009; Malanoski, Anthony/C-7814-2011; OI Lin, Baochuan/0000-0002-9484-0785; Malanoski, Anthony/0000-0001-6192-888X; Vora, Gary/0000-0002-0657-8597; Eddie, Brian/0000-0002-3559-3892 FU Office of Naval Research via U.S. NRL core funds [N0001413WX20995, N0001414WX20485, N0001414WX20518] FX This work was funded by the Office of Naval Research via U.S. NRL core funds, as well as under the following award numbers (to S.M.S.-G.): N0001413WX20995, N0001414WX20485, and N0001414WX20518. NR 72 TC 15 Z9 16 U1 7 U2 66 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JAN PY 2015 VL 81 IS 2 BP 699 EP 712 DI 10.1128/AEM.02947-14 PG 14 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AY1UL UT WOS:000347377500026 PM 25398855 ER PT J AU Schmidt, JW Agga, GE Bosilevac, JM Brichta-Harhay, DM Shackelford, SD Wang, R Wheeler, TL Arthur, TM AF Schmidt, John W. Agga, Getahun E. Bosilevac, Joseph M. Brichta-Harhay, Dayna M. Shackelford, Steven D. Wang, Rong Wheeler, Tommy L. Arthur, Terrance M. TI Occurrence of Antimicrobial-Resistant Escherichia coli and Salmonella enterica in the Beef Cattle Production and Processing Continuum SO APPLIED AND ENVIRONMENTAL MICROBIOLOGY LA English DT Article ID URINARY-TRACT-INFECTIONS; POLYMERASE-CHAIN-REACTION; DIRECT PLATING METHODS; UNITED-STATES; FOOD ANIMALS; SULFONAMIDE RESISTANCE; ANTIBIOTIC-RESISTANCE; NALIDIXIC-ACID; CTX-M; FLUOROQUINOLONE RESISTANCE AB Specific concerns have been raised that third-generation cephalosporin-resistant (3GC(r)) Escherichia coli, trimethoprim-sulfamethoxazole-resistant (COTr) E. coli, 3GC(r) Salmonella enterica, and nalidixic acid-resistant (NAL(r)) S. enterica may be present in cattle production environments, persist through beef processing, and contaminate final products. The prevalences and concentrations of these organisms were determined in feces and hides (at feedlot and processing plant), pre-evisceration carcasses, and final carcasses from three lots of fed cattle (n = 184). The prevalences and concentrations were further determined for strip loins from 103 of the carcasses. 3GC(r) Salmonella was detected on 7.6% of hides during processing and was not detected on the final carcasses or strip loins. NALr S. enterica was detected on only one hide. 3GC(r) E. coli and COTr E. coli were detected on 100.0% of hides during processing. Concentrations of 3GC(r) E. coli and COTr E. coli on hides were correlated with pre-evisceration carcass contamination. 3GC(r) E. coli and COTr E. coli were each detected on only 0.5% of final carcasses and were not detected on strip loins. Five hundred and 42 isolates were screened for extraintestinal pathogenic E. coli (ExPEC) virulence-associated markers. Only two COTr E. coli isolates from hides were ExPEC, indicating that fed cattle products are not a significant source of ExPEC causing human urinary tract infections. The very low prevalences of these organisms on final carcasses and their absence on strip loins demonstrate that current sanitary dressing procedures and processing interventions are effective against antimicrobial-resistant bacteria. C1 [Schmidt, John W.; Agga, Getahun E.; Bosilevac, Joseph M.; Brichta-Harhay, Dayna M.; Shackelford, Steven D.; Wang, Rong; Wheeler, Tommy L.; Arthur, Terrance M.] ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Schmidt, JW (reprint author), ARS, USDA, Roman L Hruska US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. EM john.w.schmidt@ars.usda.gov OI Schmidt, John/0000-0003-0494-2436 FU Nebraska Beef Council; U.S. Food and Drug Administration-National Antimicrobial Resistance Monitoring System FX This project was funded in part by a grant to J.W.S. from the Nebraska Beef Council and a grant to J.W.S. from the U.S. Food and Drug Administration-National Antimicrobial Resistance Monitoring System. NR 91 TC 5 Z9 5 U1 4 U2 21 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0099-2240 EI 1098-5336 J9 APPL ENVIRON MICROB JI Appl. Environ. Microbiol. PD JAN PY 2015 VL 81 IS 2 BP 713 EP 725 DI 10.1128/AEM.03079-14 PG 13 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AY1UL UT WOS:000347377500027 PM 25398858 ER PT J AU Cheung, AK Ng, TFF Lager, KM Alt, DP Delwart, E Pogranichniy, RM AF Cheung, Andrew K. Ng, Terry Fei Fan Lager, Kelly M. Alt, David P. Delwart, Eric Pogranichniy, Roman M. TI Identification of several clades of novel single-stranded circular DNA viruses with conserved stem-loop structures in pig feces SO ARCHIVES OF VIROLOGY LA English DT Article AB Metagenomic analysis of fecal samples collected from diarrheal swine detected sequences encoding a replication initiator protein (Rep). The genomes of ten novel single-stranded DNA viruses were determined, and they exhibited a similar genome organization. The two putative open reading frames (ORFs) encoding Rep and the capsid protein are bidirectionally transcribed and separated by two intergenic regions. Stem-loop structure(s) typical of genomes that undergo the rolling-circle DNA replication mechanism were observed. Phylogenetically, these ten genomes are in a monophyletic clade with the previously described porcine stool-associated virus (PoSCV) but are divergent enough to be further classified into to six distinct virus clades. C1 [Cheung, Andrew K.; Lager, Kelly M.] ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Ng, Terry Fei Fan; Delwart, Eric] Univ Calif San Francisco, Blood Syst Res Inst, San Francisco, CA 94143 USA. [Ng, Terry Fei Fan; Delwart, Eric] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA. [Alt, David P.] ARS, Infect Bacterial Dis Res Unit, Natl Anim Dis Ctr, USDA, Ames, IA 50010 USA. [Pogranichniy, Roman M.] Purdue Univ, Indiana Anim Dis Diagnost Lab, W Lafayette, IN 47907 USA. [Pogranichniy, Roman M.] Purdue Univ, Dept Comparat Pathobiol, W Lafayette, IN 47907 USA. RP Cheung, AK (reprint author), ARS, Virus & Prion Dis Res Unit, Natl Anim Dis Ctr, USDA, POB 70, Ames, IA 50010 USA. EM Andrew.Cheung@ars.usda.gov OI Delwart, Eric/0000-0002-6296-4484; Ng, Terry Fei Fan/0000-0002-4815-8697 NR 19 TC 6 Z9 6 U1 0 U2 17 PU SPRINGER WIEN PI WIEN PA SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA SN 0304-8608 EI 1432-8798 J9 ARCH VIROL JI Arch. Virol. PD JAN PY 2015 VL 160 IS 1 BP 353 EP 358 DI 10.1007/s00705-014-2234-9 PG 6 WC Virology SC Virology GA AY2FE UT WOS:000347403200039 PM 25248627 ER PT J AU Wurtsbaugh, WA Heredia, NA Laub, BG Meredith, CS Mohn, HE Null, SE Pluth, DA Roper, BB Saunders, WC Stevens, DK Walker, RH Wheeler, K AF Wurtsbaugh, Wayne A. Heredia, Nicholas A. Laub, Brian G. Meredith, Christy S. Mohn, Harrison E. Null, Sarah E. Pluth, David A. Roper, Brett B. Saunders, W. Carl Stevens, David K. Walker, Richard H. Wheeler, Kit TI Approaches for studying fish production: Do river and lake researchers have different perspectives? SO CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES LA English DT Article ID TROUT SALMO-TRUTTA; JUVENILE RAINBOW-TROUT; SPAWNING COHO SALMON; ADFLUVIAL BULL TROUT; FLORIDA USA LAKES; BROWN TROUT; TROPHIC STATE; GREAT-LAKES; FOOD WEBS; HABITAT RELATIONSHIPS AB Biased perspectives of fisheries researchers may hinder scientific progress and effective management if limiting factors controlling productivity go unrecognized. We investigated whether river and lake researchers used different approaches when studying salmonid production and whether any differences were ecologically supported. We assessed 564 peer-reviewed papers published between 1966 and 2012 that studied salmonid production or surrogate variables (e. g., abundance, growth, biomass, population) and classified them into five major predictor variable categories: physical habitat, fertility (i. e., nutrients, bottom-up), biotic, temperature, and pollution. The review demonstrated that river researchers primarily analyzed physical habitat (65% of studies) and lake researchers primarily analyzed fertility (45%) and biotic (51%) variables. Nevertheless, understudied variables were often statistically significant predictors of production for lake and river systems and, combined with other evidence, suggests that unjustified a priori assumptions may dictate the choice of independent variables studied. Broader consideration of potential limiting factors on fish production, greater research effort on understudied genera, and increased publication in broadly scoped journals would likely promote integration between lentic and lotic perspectives and improve fisheries management. C1 [Wurtsbaugh, Wayne A.; Heredia, Nicholas A.; Laub, Brian G.; Mohn, Harrison E.; Null, Sarah E.; Pluth, David A.; Roper, Brett B.; Saunders, W. Carl; Walker, Richard H.; Wheeler, Kit] Utah State Univ, Watershed Sci Dept, Logan, UT 84322 USA. [Wurtsbaugh, Wayne A.; Heredia, Nicholas A.; Laub, Brian G.; Mohn, Harrison E.; Null, Sarah E.; Pluth, David A.; Roper, Brett B.; Saunders, W. Carl; Walker, Richard H.; Wheeler, Kit] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. [Meredith, Christy S.; Mohn, Harrison E.; Roper, Brett B.] US Forest Serv, Logan, UT 84321 USA. [Stevens, David K.] Utah State Univ, Dept Civil & Environm Engn, Logan, UT 84322 USA. RP Wurtsbaugh, WA (reprint author), Utah State Univ, Watershed Sci Dept, Logan, UT 84322 USA. EM wayne.wurtsbaugh@usu.edu RI Null, Sarah/E-4422-2011 NR 114 TC 1 Z9 1 U1 4 U2 32 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0706-652X EI 1205-7533 J9 CAN J FISH AQUAT SCI JI Can. J. Fish. Aquat. Sci. PD JAN PY 2015 VL 72 IS 1 BP 149 EP 160 DI 10.1139/cjfas-2014-0210 PG 12 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AY0PH UT WOS:000347298300012 ER PT J AU DeRose, RJ Mock, KE Long, JN AF DeRose, R. Justin Mock, Karen E. Long, James N. TI Cytotype differences in radial increment provide novel insight into aspen reproductive ecology and stand dynamics SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE dendroecology; genetic diversity; ploidy; Populus tremuloides; silviculture ID POPULUS-TREMULOIDES; QUAKING ASPEN; GENETIC-VARIATION; GROWTH; CLIMATE; PHYTOCHEMISTRY; PRODUCTIVITY; CALIBRATION; DEFOLIATION; DROUGHT AB High rates of triploidy have recently been described in quaking aspen (Populus tremuloides Michx.) of the Intermountain West, raising questions about the contributions of triploidy to stand persistence and dynamics. In this study, we investigated cytotype differences between diploid and triploid aspen clones using dendrochronological techniques. We used tree-ring data collected from stems within an aspen stand near Fish Lake, Utah, to test for differences in stem age, population structure, growth, and response to climate. This stand contains the well-known Pando clone, which is purported to be the largest organism documented on earth. Our results show that triploid aspen stems grew more rapidly than diploids, and that this difference was most pronounced early in stand development. Growth response to climate varied little between triploids and diploids, where wide rings were associated with cool, moist years, and narrow rings were associated with above-average growing season temperatures. Stand development processes and inherent genetic differences are mechanisms possibly controlling the observed differences in aspen ring width between triploids and diploids. Regardless of the mechanism, the results have specific management implications. Conventional regeneration methods involving coppicing and the associated intermediate treatments will promote asexually reproducing triploids, leading to static or reduced genetic diversity. Enhanced genetic diversity will be favored by management actions that explicitly account for (i) the potential existence of multiple cytotypes within a stand and (ii) the observed differences in growth rates between diploid and triploid individuals. C1 [DeRose, R. Justin] US Forest Serv, USDA, Rocky Mt Res Stn, Ogden, UT 84401 USA. [Mock, Karen E.; Long, James N.] Utah State Univ, Dept Wildland Resources, Logan, UT 84322 USA. [Mock, Karen E.; Long, James N.] Utah State Univ, Ctr Ecol, Logan, UT 84322 USA. RP DeRose, RJ (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 507 25th St, Ogden, UT 84401 USA. EM rjderose@fs.fed.us FU T.W. Daniel Fellowship Endowment; USU ADVANCE program; Utah Agricultural Experiment Station, Utah State University [8564] FX This work was supported by the T.W. Daniel Fellowship Endowment, the USU ADVANCE program, and the Utah Agricultural Experiment Station, Utah State University (approved as journal paper No. 8564). This paper was prepared in part by an employee of the USDA Forest Service as part of official duties and therefore is in the public domain. NR 43 TC 2 Z9 2 U1 1 U2 18 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JAN PY 2015 VL 45 IS 1 BP 1 EP 8 DI 10.1139/cjfr-2014-0382 PG 8 WC Forestry SC Forestry GA AY0PA UT WOS:000347297500001 ER PT J AU McRoberts, RE Moser, P Oliveira, LZ Vibrans, AC AF McRoberts, Ronald E. Moser, Paolo Oliveira, Laio Zimermann Vibrans, Alexander C. TI A general method for assessing the effects of uncertainty in individual-tree volume model predictions on large-area volume estimates with a subtropical forest illustration SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE allometric model; residual uncertainty; parameter uncertainty; Monte Carlo simulation; Santa Catarina; Brazil ID ALLOMETRIC EQUATIONS; MEASUREMENT ERRORS; TROPICAL FORESTS; INVENTORY DATA; CARBON STOCKS; BIOMASS; SCALE AB Forest inventory estimates of tree volume for large areas are typically calculated by adding the model predictions of volumes for individual trees at the plot level, calculating the mean over plots, and expressing the result on a per unit area basis. The uncertainty in the model predictions is generally ignored, with the result that the precision of the large-area volume estimate is optimistic. The primary study objective was to assess the performance of a Monte Carlo based approach for estimating model prediction error that had been developed for boreal and temperate forest applications when used for a subtropical forest application. Monte Carlo simulation approaches were used because of the complexities associated with multiple sources of uncertainty, the nonlinear nature of the models, and heteroskedasticity. A related objective was to estimate the effects of model prediction uncertainty due to residual and parameter uncertainty on the large-area volume estimates for the Brazilian state of Santa Catarina. The primary conclusions were fourfold. First, the methodological approach worked well. Second, the effects of model residual and parameter uncertainty on large-area estimates of mean volume per unit area were negligible for the models and calibration datasets used for the study. Third, for the models currently in use in Santa Catarina, the effects of model residual and parameter uncertainty may be ignored when calculating large-area estimates of mean volume per unit area. Fourth, differences were negligible between estimates of the mean and standard error obtained using a single, nonspecific volume model and estimates obtained using both forest-type models and species-specific/species-group models. C1 [McRoberts, Ronald E.] US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. [Moser, Paolo; Oliveira, Laio Zimermann; Vibrans, Alexander C.] Univ Reg Blumenau, BR-89030000 Blumenau, SC, Brazil. RP McRoberts, RE (reprint author), US Forest Serv, No Res Stn, USDA, St Paul, MN 55108 USA. EM rmcroberts@fs.fed.us RI Vibrans, Alexander/C-3089-2013 OI Vibrans, Alexander/0000-0002-8789-5833 NR 28 TC 8 Z9 8 U1 1 U2 10 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JAN PY 2015 VL 45 IS 1 BP 44 EP 51 DI 10.1139/cjfr-2014-0266 PG 8 WC Forestry SC Forestry GA AY0PA UT WOS:000347297500006 ER PT J AU Halman, JM Schaberg, PG Hawley, GJ Hansen, CF Fahey, TJ AF Halman, Joshua M. Schaberg, Paul G. Hawley, Gary J. Hansen, Christopher F. Fahey, Timothy J. TI Differential impacts of calcium and aluminum treatments on sugar maple and American beech growth dynamics SO CANADIAN JOURNAL OF FOREST RESEARCH LA English DT Article DE acid deposition; dendrochronology; ice storm; tree growth; Hubbard Brook ID NORTHERN HARDWOOD FOREST; BROOK-EXPERIMENTAL-FOREST; HUBBARD-BROOK; OXIDATIVE STRESS; RED SPRUCE; UNITED-STATES; NEW-HAMPSHIRE; TREES; DEPOSITION; HEALTH AB Acid deposition induced losses of calcium (Ca) from northeastern forests have had negative effects on forest health for decades, including the mobilization of potentially phytotoxic aluminum (Al) from soils. To evaluate the impact of changes in Ca and Al availability on sugar maple (Acer saccharum Marsh.) and American beech (Fagus grandifolia Ehrh.) growth and forest composition following a major ice storm in 1998, we measured xylem annual increment, foliar cation concentrations, American beech root sprouting, and tree mortality at the Hubbard Brook Experimental Forest (Thornton, New Hampshire) in control plots and in plots amended with Ca or Al (treated plots) beginning in 1995. Dominant sugar maple trees were unaffected by the treatment, but nondominant sugar maple tree growth responded positively to Ca treatment. Although plots were mainly composed of sugar maple, American beech experienced the greatest growth on Al-treated plots. Increases in tree mortality on Al-treated plots may have released surviving American beech and increased their growth. The Al tolerance of American beech and the Ca: Al sensitivity of sugar maple contributed to divergent growth patterns that influenced stand productivity and composition. Given that acidic inputs are expected to continue, the growth dynamics associated with Al treatment may have direct relevance to future conditions in native forests. C1 [Halman, Joshua M.; Hansen, Christopher F.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, S Burlington, VT 05403 USA. [Schaberg, Paul G.] US Forest Serv, USDA, No Res Stn, Burlington, VT 05405 USA. [Hawley, Gary J.] Univ Vermont, Rubenstein Sch Environm & Nat Resources, Burlington, VT 05405 USA. [Fahey, Timothy J.] Cornell Univ, Dept Nat Resources, Ithaca, NY 14853 USA. RP Halman, JM (reprint author), Univ Vermont, Rubenstein Sch Environm & Nat Resources, 705 Spear St, S Burlington, VT 05403 USA. EM jhalman@uvm.edu FU National Science Foundation; USDA Forest Service Northern Research Station; USDA CSREES McIntire-Stennis Forest Research Program; National Research Initiative; Northeastern States Research Cooperative FX The authors are grateful to Paula Murakami, Ali Kosiba, and Ben Engel for their assistance in both the field and laboratory. We thank Brett Huggett, Kimberly Wallin, Shelly Rayback, and Donald Ross for providing helpful feedback on an earlier version of this manuscript. We further thank the staff at the Hubbard Brook Experimental Forest for their generous help in all aspects of our sample collections. This manuscript is a contribution of the Hubbard Brook Ecosystem Study. Hubbard Brook is part of the Long-Term Ecological Research (LTER) network, which is supported by the National Science Foundation. The Hubbard Brook Experimental Forest is operated and maintained by the USDA Forest Service, Northern Research Station, Newtown Square, Pennsylvania. This research was supported by funds provided by the USDA Forest Service Northern Research Station, USDA CSREES McIntire-Stennis Forest Research Program and National Research Initiative, and the Northeastern States Research Cooperative. NR 50 TC 5 Z9 5 U1 2 U2 30 PU CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS PI OTTAWA PA 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA SN 0045-5067 EI 1208-6037 J9 CAN J FOREST RES JI Can. J. For. Res. PD JAN PY 2015 VL 45 IS 1 BP 52 EP 59 DI 10.1139/cjfr-2014-0250 PG 8 WC Forestry SC Forestry GA AY0PA UT WOS:000347297500007 ER PT J AU Pelc, SE Linder, CR AF Pelc, Sandra E. Linder, C. Randal TI Emergence timing and fitness consequences of variation in seed oil composition in Arabidopsis thaliana SO ECOLOGY AND EVOLUTION LA English DT Article DE Adaptive evolution; Arabidopsis thaliana; emergence timing; fatty acids; relative fitness; seed oil composition ID ACID BETA-OXIDATION; SELECTION; DORMANCY; BODIES; PLANTS; LOCI AB Early seedling emergence can increase plant fitness under competition. Seed oil composition (the types and relative amounts of fatty acids in the oils) may play an important role in determining emergence timing and early growth rate in oilseeds. Saturated fatty acids provide more energy per carbon atom than unsaturated fatty acids but have substantially higher melting points (when chain length is held constant). This characteristic forms the basis of an adaptive hypothesis that lower melting point seeds (lower proportion of saturated fatty acids) should be favored under colder germination temperatures due to earlier germination and faster growth before photosynthesis, while at warmer germination temperatures, seeds with a higher amount of energy (higher proportion of saturated fatty acids) should be favored. To assess the effects of seed oil melting point on timing of seedling emergence and fitness, high- and low-melting point lines from a recombinant inbred cross of Arabidopsis thaliana were competed in a fully factorial experiment at warm and cold temperatures with two different density treatments. Emergence timing between these lines was not significantly different at either temperature, which aligned with warm temperature predictions, but not cold temperature predictions. Under all conditions, plants competing against high-melting point lines had lower fitness relative to those against low-melting point lines, which matched expectations for undifferentiated emergence times. C1 [Pelc, Sandra E.] ARS, US Vegetable Lab, USDA, Charleston, SC 29414 USA. [Pelc, Sandra E.; Linder, C. Randal] Univ Texas Austin, Dept Integrat Biol, Austin, TX 78712 USA. RP Pelc, SE (reprint author), US Vegetable Lab, 2700 Savannah Highway, Charleston, SC 29414 USA. EM Sandra.Pelc@ars.usda.gov FU National Science Foundation [DEB-1011609] FX This work was supported by an National Science Foundation Doctoral dissertation improvement grant DEB-1011609. NR 25 TC 0 Z9 0 U1 3 U2 23 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 2045-7758 J9 ECOL EVOL JI Ecol. Evol. PD JAN PY 2015 VL 5 IS 1 BP 164 EP 171 DI 10.1002/ece3.1265 PG 8 WC Ecology; Evolutionary Biology SC Environmental Sciences & Ecology; Evolutionary Biology GA AY3ZF UT WOS:000347517300015 PM 25628873 ER PT J AU Thompson, MP Haas, JR Gilbertson-Day, JW Scott, JH Langowski, P Bowne, E Calkin, DE AF Thompson, Matthew P. Haas, Jessica R. Gilbertson-Day, Julie W. Scott, Joe H. Langowski, Paul Bowne, Elise Calkin, David E. TI Development and application of a geospatial wildfire exposure and risk calculation tool SO ENVIRONMENTAL MODELLING & SOFTWARE LA English DT Article DE Decision support; Exposure analysis; Effects analysis; GIS; Risk assessment; Wildfire ID WILDLAND FIRE; UNITED-STATES; DECISION-MAKING; NATIONAL FOREST; DEBRIS FLOWS; MANAGEMENT; MODELS; SYSTEM; IMPLEMENTATION; PROBABILITY AB Applying wildfire risk assessment models can inform investments in loss mitigation and landscape restoration, and can be used to monitor spatiotemporal trends in risk. Assessing wildfire risk entails the integration of fire modeling outputs, maps of highly valued resources and assets (HVRAs), characterization of fire effects, and articulation of relative importance across HVRAs. Quantifying and geoprocessing wildfire risk can be a complex and time-intensive task, often requiring expertise in geospatial analysis. Researchers and land managers alike would benefit from a standardized and streamlined ability to estimate wildfire risk. In this paper we present the development and application of a geospatial wildfire risk calculation tool, FireNVC. We describe the major components of the tool and how they align with a geospatial wildfire risk assessment framework, detail a recent application of the tool to inform federal wildfire management and planning, and offer suggestions for future improvements and uses of the tool. Published by Elsevier Ltd. C1 [Thompson, Matthew P.; Haas, Jessica R.; Gilbertson-Day, Julie W.; Calkin, David E.] US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59812 USA. [Scott, Joe H.] Pyrologix LLC, Missoula, MT USA. [Langowski, Paul; Bowne, Elise] US Forest Serv, Missoula, MT USA. RP Thompson, MP (reprint author), US Forest Serv, Rocky Mt Res Stn, Missoula, MT 59812 USA. EM mpthompson02@fs.fed.us FU Rocky Mountain Research Station; National Fire Decision Support Center FX The Rocky Mountain Research Station and the National Fire Decision Support Center supported this research. Thanks to Jim Menakis for his efforts supporting this process. NR 50 TC 11 Z9 11 U1 0 U2 28 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1364-8152 EI 1873-6726 J9 ENVIRON MODELL SOFTW JI Environ. Modell. Softw. PD JAN PY 2015 VL 63 BP 61 EP 72 DI 10.1016/j.envsoft.2014.09.018 PG 12 WC Computer Science, Interdisciplinary Applications; Engineering, Environmental; Environmental Sciences SC Computer Science; Engineering; Environmental Sciences & Ecology GA AY1OT UT WOS:000347362900006 ER PT J AU Aguer, C McCoin, CS Knotts, TA Thrush, AB Ono-Moore, K McPherson, R Dent, R Hwang, DH Adams, SH Harper, ME AF Aguer, Celine McCoin, Colin S. Knotts, Trina A. Thrush, A. Brianne Ono-Moore, Kikumi McPherson, Ruth Dent, Robert Hwang, Daniel H. Adams, Sean H. Harper, Mary-Ellen TI Acylcarnitines: potential implications for skeletal muscle insulin resistance SO FASEB JOURNAL LA English DT Article DE fatty acid beta-oxidation; mitochondria; inflammation; oxidative stress; myotubes ID CARNITINE ACETYLTRANSFERASE; SUPEROXIDE-PRODUCTION; GLUCOSE-TOLERANCE; BETA-OXIDATION; FAT OXIDATION; INHIBITION; PALMITATE; HUMANS; CELLS; EXPRESSION AB Insulin resistance may be linked to incomplete fatty acid beta-oxidation and the subsequent increase in acylcarnitine species in different tissues including skeletal muscle. It is not known if acylcarnitines participate in muscle insulin resistance or simply reflect dysregulated metabolism. The aims of this study were to determine whether acylcarnitines can elicit muscle insulin resistance and to better understand the link between incomplete muscle fatty acid beta-oxidation, oxidative stress, inflammation, and insulin-resistance development. Differentiated C2C12, primary mouse, and human myotubes were treated with acylcarnitines (C4: 0, C14: 0, C16: 0) or with palmitate with or without carnitine acyltransferase inhibition by mildronate. Treatment with C4: 0, C14: 0, and C16: 0 acylcarnitines resulted in 20-30% decrease in insulin response at the level of Akt phosphorylation and/or glucose uptake. Mildronate reversed palmitate-induced insulin resistance concomitant with an similar to 25% decrease in short-chain acylcarnitine and acetylcarnitine secretion. Although proinflammatory cytokines were not affected under these conditions, oxidative stress was increased by 2-3 times by short-or long-chain acylcarnitines. Acylcarnitine-induced oxidative stress and insulin resistance were reversed by treatment with antioxidants. Results are consistent with the conclusion that incomplete muscle fatty acid beta-oxidation causes acylcarnitine accumulation and associated oxidative stress, raising the possibility that these metabolites play a role in muscle insulin resistance. C1 [Aguer, Celine; Thrush, A. Brianne; Harper, Mary-Ellen] Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada. [McCoin, Colin S.; Adams, Sean H.] Univ Calif Davis, Mol Cellular & Integrat Physiol Grad Program, Davis, CA 95616 USA. [McCoin, Colin S.; Knotts, Trina A.; Ono-Moore, Kikumi; Adams, Sean H.] ARS, Obes & Metab Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA USA. [Knotts, Trina A.; Ono-Moore, Kikumi; Hwang, Daniel H.; Adams, Sean H.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [McPherson, Ruth] Univ Ottawa Heart Inst, Div Cardiol, Ottawa, ON, Canada. [Dent, Robert] Ottawa Hosp, Weight Management Clin, Ottawa, ON, Canada. [Hwang, Daniel H.] ARS, Immun & Dis Prevent Res Unit, USDA, Western Human Nutr Res Ctr, Davis, CA USA. RP Harper, ME (reprint author), Univ Ottawa, Fac Med, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada. EM seanharrisonadams@gmail.com; mharper@uottawa.ca OI McCoin, Colin/0000-0002-8557-6441; Harper, Mary-Ellen/0000-0003-3864-5886 FU Canadian Institutes of Health Research [MOP57810]; NIDDK NIH HHS [R01 DK078328, R01DK078328-01, R01DK078328-02S1] NR 45 TC 22 Z9 22 U1 4 U2 12 PU FEDERATION AMER SOC EXP BIOL PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3998 USA SN 0892-6638 EI 1530-6860 J9 FASEB J JI Faseb J. PD JAN PY 2015 VL 29 IS 1 BP 336 EP 345 DI 10.1096/fj.14-255901 PG 10 WC Biochemistry & Molecular Biology; Biology; Cell Biology SC Biochemistry & Molecular Biology; Life Sciences & Biomedicine - Other Topics; Cell Biology GA AY1UX UT WOS:000347378600031 PM 25342132 ER PT J AU Baker, MS Li, G Kohorst, JJ Waterland, RA AF Baker, M. S. Li, G. Kohorst, J. J. Waterland, R. A. TI Fetal growth restriction promotes physical inactivity and obesity in female mice SO INTERNATIONAL JOURNAL OF OBESITY LA English DT Article DE developmental origins; developmental programming; body weight regulation; physical activity; energy expenditure; metabolic imprinting ID AGOUTI GENE-EXPRESSION; ENERGY-EXPENDITURE; HYPERCALORIC NUTRITION; MATERNAL OBESITY; WEIGHT-GAIN; PREGNANCY; EXPOSURE; TARGETS; FAMINE; MOUSE AB BACKGROUND: Environmental exposures during critical periods of prenatal and early postnatal life affect the development of mammalian body weight regulatory mechanisms, influencing lifelong risk of obesity. The specific biological processes that mediate the persistence of such effects, however, remain poorly understood. OBJECTIVE: The objectives of this study were to determine the developmental timing and physiological basis of the obesity-promoting effect previously reported in offspring of obese agouti viable yellow (A(vy)/a) mothers. DESIGN: Newborn offspring of obese A(vy)/a and lean (a/a) mothers were cross-fostered shortly after birth to study separately the effects of in utero or suckling period exposure to A(vy)/a dams. Body composition, food intake, physical activity and energy expenditure were measured in offspring shortly after weaning and in adulthood. RESULTS: Offspring of obese A(vy)/a dams paradoxically experienced fetal growth restriction, which was followed by adult-onset obesity specifically in females. Our main analyses focused on wild-type (a/a) offspring, because a subset of adult A(vy)/a offspring contracted a kidney disease resembling diabetic nephropathy. Detailed physiological characterization demonstrated that, both shortly after weaning and in adulthood, female wild-type mice born to A(vy)/a mothers are not hyperphagic but have reduced physical activity and energy expenditure. No such coordinated changes were detected in male offspring. Mediational regression analysis of our longitudinal data supported a causal pathway in which fetal growth restriction persistently reduces physical activity, leading to adult obesity. CONCLUSIONS: Our data are consistent with several recent human epidemiological studies showing female-specific effects of perinatal nutritional restriction on later obesity, and provide the novel mechanistic insight that this may occur via permanent and sex-specific changes in one's inherent propensity for physical activity. C1 [Baker, M. S.; Li, G.; Kohorst, J. J.; Waterland, R. A.] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, Houston, TX 77030 USA. [Waterland, R. A.] Baylor Coll Med, Dept Mol & Human Genet, Houston, TX 77030 USA. RP Waterland, RA (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Pediat, 1100 Bates St,Ste 5080, Houston, TX 77030 USA. EM waterland@bcm.edu OI Li, Ge/0000-0002-6575-8969 FU NIH/NIDDK [1R01DK081557]; USDA (CRIS) [6250-51000-055]; USDA/ARS FX We thank Adam Gillum (USDA/ARS CNRC) for assistance with the figures and Firoz Vohra (USDA/ARS CNRC) for assistance with the CLAMS studies. MSB conducted research and wrote the paper; GL and JJK conducted research; RAW designed research, analyzed data, wrote the paper and had primary responsibility for final content. This work was supported by grants from NIH/NIDDK (1R01DK081557) and USDA (CRIS 6250-51000-055) to RAW. The body composition and CLAMS studies were performed in the Mouse Metabolic Research Unit at the USDA/ARS Children's Nutrition Research Center, which is supported by funds from the USDA/ARS. NR 47 TC 5 Z9 5 U1 1 U2 5 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 0307-0565 EI 1476-5497 J9 INT J OBESITY JI Int. J. Obes. PD JAN PY 2015 VL 39 IS 1 BP 98 EP 104 DI 10.1038/ijo.2013.146 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AY2BR UT WOS:000347394000015 PM 23924758 ER PT J AU Caruso, ML Cullen, KW AF Caruso, Michelle L. Cullen, Karen W. TI Quality and Cost of Student Lunches Brought From Home SO JAMA PEDIATRICS LA English DT Article ID PACKED LUNCHES; CHILDREN; FOOD; BAG AB IMPORTANCE The nutritional quality and cost of lunches brought from home are overlooked and understudied aspects of the school food environment. OBJECTIVES To examine the quality and cost of lunches brought from home by elementary and intermediate school students. DESIGN, SETTING, AND PARTICIPANTS An observational studywas conducted in 12 schools (8 elementary and 4 intermediate) in one Houston, Texas, area school district from October 6, 2011, to December 5, 2011. Participants included 242 elementary and 95 intermediate school students who brought lunches from home. EXPOSURES Lunches brought from home. MAIN OUTCOMES AND MEASURES Foods brought and amounts eaten were recorded along with student grade level and sex. Nutrient and food group content were calculated and compared with current National School Lunch Program (NSLP) guidelines. Per-serving prices for each item were collected from 3 grocery stores in the study area and averaged. RESULTS Compared with the NSLP guidelines, lunches brought from home contained more sodium (1110 vs <= 640 mg for elementary and 1003 vs <= 710 mg for intermediate students) and fewer servings of fruits (0.33 cup for elementary and 0.29 cup for intermediate students vs 0.50 cup per the NSLP guidelines), vegetables (0.07 cup for elementary and 0.11 cup for intermediate students vs 0.75 cup per the NSLP guidelines), whole grains (0.22-oz equivalent for elementary and 0.31-oz equivalent for intermediate students vs 0.50-oz minimum per the NLSP guidelines), and fluid milk (0.08 cup for elementary and 0.02 cup for intermediate students vs 1 cup per the NSLP guidelines). About 90% of lunches from home contained desserts, snack chips, and sweetened beverages, which are not permitted in reimbursable school meals. The cost of lunches from home averaged $1.93 for elementary and $1.76 for intermediate students. Students from lower-income intermediate schools brought significantly higher-priced ($1.94) lunches than did students from middle-income schools ($1.63). CONCLUSIONS AND RELEVANCE Lunches brought from home compared unfavorably with current NSLP guidelines. Strategies are needed to improve the nutritional quality of lunches brought from home. C1 [Caruso, Michelle L.] Dept Hlth & Human Serv, Houston, TX 77054 USA. [Cullen, Karen W.] Baylor Coll Med, Childrens Nutr Res Ctr, ARS, Dept Pediat,USDA, Houston, TX 77030 USA. RP Caruso, ML (reprint author), Dept Hlth & Human Serv, 8000 N Stadium Dr,8th Floor, Houston, TX 77054 USA. EM michelle.caruso@houstontx.gov FU USDA/ARS [6250-51000-053]; Eunice Kennedy Shriver National Institute of Child Health and Development [R01HD068349] FX This work is a publication of the US Department of Agriculture (USDA)/Agricultural Research Service (ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas. This project has been funded in part by federal funds from the USDA/ARS under Cooperative Agreement No. 6250-51000-053. This work was also supported by grant R01HD068349 from the Eunice Kennedy Shriver National Institute of Child Health and Development (Dr Cullen). NR 10 TC 6 Z9 6 U1 0 U2 14 PU AMER MEDICAL ASSOC PI CHICAGO PA 330 N WABASH AVE, STE 39300, CHICAGO, IL 60611-5885 USA SN 2168-6203 EI 2168-6211 J9 JAMA PEDIATR JI JAMA Pediatr. PD JAN PY 2015 VL 169 IS 1 BP 86 EP 90 DI 10.1001/jamapediatrics.2014.2220 PG 5 WC Pediatrics SC Pediatrics GA AY1JI UT WOS:000347349300019 PM 25419622 ER PT J AU Hakki, SS Malkoc, S Dundar, N Kayis, SA Hakki, EE Hamurcu, M Baspinar, N Basoglu, A Nielsen, FH Gotz, W AF Hakki, Sema S. Malkoc, Siddik Dundar, Niyazi Kayis, Seyit Ali Hakki, Erdogan E. Hamurcu, Mehmet Baspinar, Nuri Basoglu, Abdullah Nielsen, Forrest H. Goetz, Werner TI Dietary boron does not affect tooth strength, micro-hardness, and density, but affects tooth mineral composition and alveolar bone mineral density in rabbits fed a high-energy diet SO JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY LA English DT Article DE Boron; Teeth; Alveolar bone; Teeth composition; Minerals ID TRACE-ELEMENTS; DEFICIENT DIET; DENTAL ENAMEL; RATS; CARIES; DIFFERENTIATION; INFLAMMATION; INCISORS AB The objective of this study was to determine whether dietary boron (B) affects the strength, density and mineral composition of teeth and mineral density of alveolar bone in rabbits with apparent obesity induced by a high-energy diet. Sixty female, 8-month-old, New Zealand rabbits were randomly assigned for 7 months into five groups as follows: (1) control 1, fed alfalfa hay only (5.91 MJ/kg and 57.5 mg B/kg); (2) control 2, high energy diet (11.76 MJ and 3.88 mg B/kg); (3) B10, high energy diet + 10 mg B gavage/kg body weight/96 h; (4) B30, high energy diet + 30 mg B gavage/kg body weight/96 h; (5) B50, high energy diet + 50 mg B gavage/kg body weight/96 h. Maxillary incisor teeth of the rabbits were evaluated for compression strength, mineral composition, and micro-hardness. Enamel, dentin, cementum and pulp tissue were examined histologically. Mineral densities of the incisor teeth and surrounding alveolar bone were determined by using micro-CT. When compared to controls, the different boron treatments did not significantly affect compression strength, and micro-hardness of the teeth, although the B content of teeth increased in a dose-dependent manner. Compared to control 1, B50 teeth had decreased phosphorus (P) concentrations. Histological examination revealed that teeth structure (shape and thickness of the enamel, dentin, cementum and pulp) was similar in the B-treated and control rabbits. Micro CT evaluation revealed greater alveolar bone mineral density in B10 and B30 groups than in controls. Alveolar bone density of the B50 group was not different than the controls. Although the B treatments did not affect teeth structure, strength, mineral density and micro-hardness, increasing B intake altered the mineral composition of teeth, and, in moderate amounts, had beneficial effects on surrounding alveolar bone. (C) 2014 Elsevier GmbH. All rights reserved. C1 [Hakki, Sema S.] Selcuk Univ, Fac Dent, Dept Periodontol, TR-42079 Konya, Turkey. [Malkoc, Siddik] Inonu Univ, Dept Orthodont, Fac Dent, Malatya, Turkey. [Hakki, Sema S.; Dundar, Niyazi] Selcuk Univ, Res Ctr, Fac Dent, Konya, Turkey. [Kayis, Seyit Ali] Selcuk Univ, Dept Anim Sci, Fac Agr, Konya, Turkey. [Hakki, Erdogan E.; Hamurcu, Mehmet] Selcuk Univ, Dept Soil Sci & Plant Nutr, Fac Agr, Konya, Turkey. [Baspinar, Nuri] Selcuk Univ, Fac Vet Med, Dept Biochem, Konya, Turkey. [Basoglu, Abdullah] Selcuk Univ, Dept Internal Med, Fac Vet Med, Konya, Turkey. [Nielsen, Forrest H.] ARS, USDA, Grand Forks Human Nutr Res Ctr, Grand Forks, ND 58202 USA. [Goetz, Werner] Univ Bonn, Fac Med, Dept Orthodont, Oral Biol Lab, Bonn, Germany. RP Hakki, SS (reprint author), Selcuk Univ, Fac Dent, Dept Periodontol, TR-42079 Konya, Turkey. EM sshakki@selcuk.edu.tr FU Turkish National Boron Research Institute, Ankara, Turkey [BOREN-2006-09-C08] FX Authors thank to A. Sagkan Ozturk and P. Peker Akalin for their technical assistance for the animal experiments. This study was supported by Turkish National Boron Research Institute (BOREN-2006-09-C08), Ankara, Turkey. NR 43 TC 0 Z9 0 U1 2 U2 20 PU ELSEVIER GMBH, URBAN & FISCHER VERLAG PI JENA PA OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY SN 0946-672X J9 J TRACE ELEM MED BIO JI J. Trace Elem. Med. Biol. PY 2015 VL 29 BP 208 EP 215 DI 10.1016/j.jtemb.2014.10.007 PG 8 WC Biochemistry & Molecular Biology; Endocrinology & Metabolism SC Biochemistry & Molecular Biology; Endocrinology & Metabolism GA AY3QO UT WOS:000347498000027 PM 25468191 ER PT J AU Cushman, SA AF Cushman, Samuel A. TI Thermodynamics in landscape ecology: the importance of integrating measurement and modeling of landscape entropy SO LANDSCAPE ECOLOGY LA English DT Editorial Material ID ORDER C1 US Forest Serv, USDA, Rocky Mt Res Stn, Flagstaff, AZ 86001 USA. RP Cushman, SA (reprint author), US Forest Serv, USDA, Rocky Mt Res Stn, 2500 S Pine Knoll Dr, Flagstaff, AZ 86001 USA. EM scushman@fs.fed.us NR 7 TC 1 Z9 1 U1 2 U2 9 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0921-2973 EI 1572-9761 J9 LANDSCAPE ECOL JI Landsc. Ecol. PD JAN PY 2015 VL 30 IS 1 BP 7 EP 10 DI 10.1007/s10980-014-0108-x PG 4 WC Ecology; Geography, Physical; Geosciences, Multidisciplinary SC Environmental Sciences & Ecology; Physical Geography; Geology GA AY0KD UT WOS:000347284600002 ER PT J AU Law, MY Childs, KL Campbell, MS Stein, JC Olson, AJ Holt, C Panchy, N Lei, JK Jiao, D Andorf, CM Lawrence, CJ Ware, D Shiu, SH Sun, YN Jiang, N Yandell, M AF Law, MeiYee Childs, Kevin L. Campbell, Michael S. Stein, Joshua C. Olson, Andrew J. Holt, Carson Panchy, Nicholas Lei, Jikai Jiao, Dian Andorf, Carson M. Lawrence, Carolyn J. Ware, Doreen Shiu, Shin-Han Sun, Yanni Jiang, Ning Yandell, Mark TI Automated Update, Revision, and Quality Control of the Maize Genome Annotations Using MAKER-P Improves the B73 RefGen_v3 Gene Models and Identifies New Genes SO PLANT PHYSIOLOGY LA English DT Article ID RNA-SEQ DATA; TOOL; ARABIDOPSIS; SEQUENCE; MANAGEMENT; EVOLUTION; EXPANSION; DATABASE; DNA; MICRORNAS AB The large size and relative complexity of many plant genomes make creation, quality control, and dissemination of high-quality gene structure annotations challenging. In response, we have developed MAKER-P, a fast and easy-to-use genome annotation engine for plants. Here, we report the use of MAKER-P to update and revise the maize (Zea mays) B73 RefGen_v3 annotation build (5b+) in less than 3 h using the iPlant Cyberinfrastructure. MAKER-P identified and annotated 4,466 additional, well-supported protein-coding genes not present in the 5b+ annotation build, added additional untranslated regions to 1,393 5b+ gene models, identified 2,647 5b+ gene models that lack any supporting evidence (despite the use of large and diverse evidence data sets), identified 104,215 pseudogene fragments, and created an additional 2,522 noncoding gene annotations. We also describe a method for de novo training of MAKER-P for the annotation of newly sequenced grass genomes. Collectively, these results lead to the 6a maize genome annotation and demonstrate the utility of MAKER-P for rapid annotation, management, and quality control of grasses and other difficult-to-annotate plant genomes. C1 [Law, MeiYee] Jackson Lab, Bar Harbor, ME 04609 USA. [Law, MeiYee; Campbell, Michael S.; Yandell, Mark] Univ Utah, Eccles Inst Human Genet, Salt Lake City, UT 84112 USA. [Law, MeiYee] Univ Utah, Dept Biomed Informat, Salt Lake City, UT 84112 USA. [Holt, Carson; Yandell, Mark] Univ Utah, USTAR Ctr Genet Discovery, Salt Lake City, UT 84112 USA. [Panchy, Nicholas; Shiu, Shin-Han; Jiang, Ning] Michigan State Univ, Genet Program, E Lansing, MI 48824 USA. [Childs, Kevin L.; Shiu, Shin-Han] Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA. [Lei, Jikai; Sun, Yanni] Michigan State Univ, Dept Comp Sci & Engn, E Lansing, MI 48824 USA. [Jiang, Ning] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Stein, Joshua C.; Olson, Andrew J.; Ware, Doreen] Cold Spring Harbor Lab, IPlant Collaborat, Cold Spring Harbor, NY 11724 USA. [Holt, Carson] Ontario Inst Canc Res, Toronto, ON M5G 1L7, Canada. [Jiao, Dian] Univ Texas Austin, Texas Adv Comp Ctr, Austin, TX 78758 USA. [Lawrence, Carolyn J.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [Lawrence, Carolyn J.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. [Andorf, Carson M.] Iowa State Univ, Agr Res Serv Corn Insects & Crop Genet Res, USDA, Ames, IA 50011 USA. [Ware, Doreen] ARS, USDA, Northeast Area, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Yandell, M (reprint author), Univ Utah, Eccles Inst Human Genet, Salt Lake City, UT 84112 USA. EM myandell@genetics.utah.edu RI Childs, Kevin/C-9513-2014; Jiang, Ning/G-6546-2012; OI Childs, Kevin/0000-0002-3680-062X; Lei, Jikai/0000-0002-6081-2276; Shiu, Shin-Han/0000-0001-6470-235X FU National Science Foundation [IOS-1126998, MCB-1119778]; U.S. Department of Agriculture-Agricultural Research Service, and Iowa State University FX This work was supported by the National Science Foundation (grant no. IOS-1126998 to S.-H.S., Y.S., N.J., K.L.C., and M.Y. and grant no. MCB-1119778 to S.-H.S.), the U.S. Department of Agriculture-Agricultural Research Service, and Iowa State University (MaizeGDB and contributions by C.M. A. and C.J.L.). NR 45 TC 12 Z9 12 U1 0 U2 13 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JAN PY 2015 VL 167 IS 1 BP 25 EP 39 DI 10.1104/pp.114.245027 PG 15 WC Plant Sciences SC Plant Sciences GA AX8PE UT WOS:000347170300002 PM 25384563 ER PT J AU Torres-Ruiz, JM Jansen, S Choat, B McElrone, AJ Cochard, H Brodribb, TJ Badel, E Burlett, R Bouche, PS Brodersen, CR Li, S Morris, H Delzon, S AF Torres-Ruiz, Jose M. Jansen, Steven Choat, Brendan McElrone, Andrew J. Cochard, Herve Brodribb, Timothy J. Badel, Eric Burlett, Regis Bouche, Pauline S. Brodersen, Craig R. Li, Shan Morris, Hugh Delzon, Sylvain TI Direct X-Ray Microtomography Observation Confirms the Induction of Embolism upon Xylem Cutting under Tension SO PLANT PHYSIOLOGY LA English DT Article ID LAURUS-NOBILIS; VULNERABILITY; CAVITATION; STEMS; PRESSURES; RECOVERY; ARTIFACT; DROUGHT; PLANTS; PLC C1 [Torres-Ruiz, Jose M.; Burlett, Regis; Bouche, Pauline S.; Delzon, Sylvain] Univ Bordeaux, BIOGECO, Unite Mixte Rech 1202, F-33615 Pessac, France. [Torres-Ruiz, Jose M.; Burlett, Regis; Bouche, Pauline S.; Delzon, Sylvain] INRA, Unite Mixte Rech BIOGECO 1202, F-33610 Cestas, France. [Jansen, Steven; Bouche, Pauline S.; Li, Shan; Morris, Hugh] Univ Ulm, Inst Systemat Bot & Ecol, D-89081 Ulm, Germany. [Choat, Brendan] Univ Western Sydney, Hawkesbury Inst Environm, Richmond, NSW 2753, Australia. [McElrone, Andrew J.] Univ Calif Davis, USDA, ARS, Crops Pathol & Genet Res Unit, Davis, CA 95616 USA. [Cochard, Herve; Badel, Eric] INRA, Unite Mixte Rech PIAF 547, F-63100 Clermont Ferrand, France. [Cochard, Herve; Badel, Eric] Blaise Pascal Univ, Clermont Univ, Unite Mixte Rech PIAF 547, F-63000 Clermont Ferrand, France. [Brodribb, Timothy J.] Univ Tasmania, Sch Plant Sci, Hobart, Tas 7001, Australia. [Brodersen, Craig R.] Yale Univ, Sch Forestry & Environm Studies, New Haven, CT 06511 USA. RP Torres-Ruiz, JM (reprint author), Univ Bordeaux, BIOGECO, Unite Mixte Rech 1202, F-33615 Pessac, France. EM torresruizjm@gmail.com RI Brodribb, Tim/C-6797-2013; Jansen, Steven/A-9868-2012; Torres-Ruiz, Jose Manuel/I-6587-2012; Brodersen, Craig/J-1112-2016; Cochard, Herve/P-7406-2015 OI Brodribb, Tim/0000-0002-4964-6107; Jansen, Steven/0000-0002-4476-5334; Torres-Ruiz, Jose Manuel/0000-0003-1367-7056; Brodersen, Craig/0000-0002-0924-2570; Cochard, Herve/0000-0002-2727-7072 FU Alexander von Humboldt Foundation; PitBulles project (French National Research Agency) [2010 Blanc 171001]; China Scholarship Council; German Research Foundation FX This work was supported by the Alexander von Humboldt Foundation (fellowship to B.C.), the PitBulles project (French National Research Agency grant no. 2010 Blanc 171001 to H.C. and E.B.), the China Scholarship Council (to S.L.), and the German Research Foundation (to S.J. and H.M.). NR 21 TC 28 Z9 32 U1 1 U2 30 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JAN PY 2015 VL 167 IS 1 BP 40 EP 43 DI 10.1104/pp.114.249706 PG 4 WC Plant Sciences SC Plant Sciences GA AX8PE UT WOS:000347170300003 PM 25378693 ER PT J AU Chen, SY Lang, P Chronis, D Zhang, S De Jong, WS Mitchum, MG Wang, XH AF Chen, Shiyan Lang, Ping Chronis, Demosthenis Zhang, Sheng De Jong, Walter S. Mitchum, Melissa G. Wang, Xiaohong TI In Planta Processing and Glycosylation of a Nematode CLAVATA3/ENDOSPERM SURROUNDING REGION- Like Effector and Its Interaction with a Host CLAVATA2-Like Receptor to Promote Parasitism SO PLANT PHYSIOLOGY LA English DT Article ID HETERODERA-GLYCINES; GLOBODERA-ROSTOCHIENSIS; ARABIDOPSIS-THALIANA; CLE PEPTIDES; ARTIFICIAL MICRORNAS; GENE; KINASE; MECHANISMS; PROTEINS; SEQUENCE AB Like other biotrophic plant pathogens, plant- parasitic nematodes secrete effector proteins into host cells to facilitate infection. Effector proteins that mimic plant CLAVATA3/ ENDOSPERM SURROUNDING REGION- related (CLE) proteins have been identified in several cyst nematodes, including the potato cyst nematode (PCN); however, the mechanistic details of this crosskingdom mimicry are poorly understood. Plant CLEs are posttranslationally modified and proteolytically processed to function as bioactive ligands critical to various aspects of plant development. Using ectopic expression coupled with nanoliquid chromatography- tandem mass spectrometry analysis, we show that the in planta mature form of proGrCLE1, a multidomain CLE effector secreted by PCN during infection, is a 12- amino acid arabinosylated glycopeptide (named GrCLE1- 1Hyp4,7g) with striking structural similarity to mature plant CLE peptides. This glycopeptide is more resistant to hydrolytic degradation and binds with higher affinity to a CLAVATA2- like receptor (StCLV2) from potato (Solanum tuberosum) than its nonglycosylated forms. We further show that StCLV2 is highly up- regulated at nematode infection sites and that transgenic potatoes with reduced StCLV2 expression are less susceptible to PCN infection, indicating that interference of the CLV2- mediated signaling pathway confers nematode resistance in crop plants. These results strongly suggest that phytonematodes have evolved to utilize host cellular posttranslational modification and processing machinery for the activation of CLE effectors following secretion into plant cells and highlight the significance of arabinosylation in regulating nematode CLE effector activity. Our finding also provides evidence that multidomain CLEs are modified and processed similarly to single- domain CLEs, adding new insight into CLE maturation in plants. C1 [Chen, Shiyan; Lang, Ping; Wang, Xiaohong] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. [Zhang, Sheng] Cornell Univ, Prote & Mass Spectrometry Facil, Inst Biotechnol & Life Sci Technol, Ithaca, NY 14853 USA. [De Jong, Walter S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Chronis, Demosthenis; Wang, Xiaohong] ARS, Robert W Holley Ctr Agr & Hlth, USDA, Ithaca, NY 14853 USA. [Mitchum, Melissa G.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Mitchum, Melissa G.] Univ Missouri, Bond Life Sci Ctr, Columbia, MO 65211 USA. RP Wang, XH (reprint author), Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Ithaca, NY 14853 USA. EM xw57@cornell.edu OI Wang, Xiaohong/0000-0003-1790-4084 FU U. S. Department of Agriculture, Agriculture and Food Research Initiative [2007- 3560717790, 009- 35302- 05304] FX This work was supported by the U. S. Department of Agriculture, Agriculture and Food Research Initiative ( grant nos. 2007- 3560717790 and 2009- 35302- 05304 to M. G. M. and X. W.). NR 62 TC 7 Z9 9 U1 5 U2 28 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 0032-0889 EI 1532-2548 J9 PLANT PHYSIOL JI Plant Physiol. PD JAN PY 2015 VL 167 IS 1 BP 262 EP + DI 10.1104/pp.114.251637 PG 17 WC Plant Sciences SC Plant Sciences GA AX8PE UT WOS:000347170300018 PM 25416475 ER PT J AU Susta, L Jones, MEB Cattoli, G Cardenas-Garcia, S Miller, PJ Brown, CC Afonso, CL AF Susta, L. Jones, M. E. B. Cattoli, G. Cardenas-Garcia, S. Miller, P. J. Brown, C. C. Afonso, C. L. TI Pathologic Characterization of Genotypes XIV and XVII Newcastle Disease Viruses and Efficacy of Classical Vaccination on Specific Pathogen-Free Birds SO VETERINARY PATHOLOGY LA English DT Article DE velogenic viscerotropic Newcastle disease virus; chickens; pathogenesis; clinicopathologic assessment; genotypes XIV and XVII; West Africa; heterologous vaccination ID PROTEIN CLEAVAGE SITE; GENETIC DIVERSITY; COMPLETE GENOME; WEST-AFRICA; VIRULENCE; OUTBREAKS; CHICKENS; SEQUENCE; STRAINS; NIGERIA AB To characterize the clinicopathologic features of recently described genotypes of Newcastle disease virus (NDV), 1 representative strain of genotype XIV and 2 of genotype XVII, all isolated from West Africa, were used to infect groups of ten 4-week-old specific pathogen-free chickens. The pathobiology of these 3 strains was compared to a South African NDV strain classified within genotype VII. All chickens infected with the 4 viruses died or were euthanized by day 4 postinfection due to the severity of clinical signs. Gross and histologic lesions in all infected chickens included extensive necrosis of lymphoid tissues (thymus, spleen, bursa of Fabricius, cecal tonsils, gut-associated lymphoid tissue), gastrointestinal necrosis and hemorrhages, and severe hemorrhagic conjunctivitis. Immunohistochemical staining revealed systemic viral distribution, and the most intense staining was in the lymphoid organs. Results demonstrate that the 3 West African strains from the previously uncharacterized genotypes XIV and XVII are typical velogenic viscerotropic NDV strains with lesions similar to the South African strain. Under experimental conditions, QV4 and LaSota NDV vaccine strains successfully protected chickens from morbidity and mortality against the genotype VII and one genotype XVII NDV strain, with no significant differences in the amount of virus shed when 2 vaccine schemes were compared. C1 [Susta, L.; Cardenas-Garcia, S.; Miller, P. J.; Afonso, C. L.] USDA ARS, SE Poultry Res Lab, Exot & Emerging Avian Viral Dis Res Unit, Athens, GA 30605 USA. [Jones, M. E. B.; Brown, C. C.] Univ Georgia, Coll Vet Med, Dept Pathol, Athens, GA 30602 USA. [Cattoli, G.] Ist Zooprofilatt Sperimentale Venezie, OIE FAO Reference Lab Avian Influenza & Newcastle, Padua, Italy. RP Susta, L (reprint author), USDA ARS, SE Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM Leonardo.susta@ars.usda.gov FU USDA-ARS [CRIS 6612-32000-064] FX The author(s) declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: This research was supported by USDA-ARS CRIS 6612-32000-064. NR 46 TC 6 Z9 6 U1 0 U2 7 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 0300-9858 EI 1544-2217 J9 VET PATHOL JI Vet. Pathol. PD JAN PY 2015 VL 52 IS 1 BP 120 EP 131 DI 10.1177/0300985814521247 PG 12 WC Pathology; Veterinary Sciences SC Pathology; Veterinary Sciences GA AY0QC UT WOS:000347300600015 PM 24510948 ER PT J AU Dashti, HS Follis, JL Smith, CE Tanaka, T Cade, BE Gottlieb, DJ Hruby, A Jacques, PF Lamon-Fava, S Richardson, K Saxena, R Scheer, FAJL Kovanen, L Bartz, TM Perala, MM Jonsson, A Frazier-Wood, AC Kalafati, IP Mikkila, V Partonen, T Lemaitre, RN Lahti, J Hernandez, DG Toft, U Johnson, WC Kanoni, S Raitakari, OT Perola, M Psaty, BM Ferrucci, L Grarup, N Highland, HM Rallidis, L Kahonen, M Havulinna, AS Siscovick, DS Raikkonen, K Jorgensen, T Rotter, JI Deloukas, P Viikari, JSA Mozaffarian, D Linneberg, A Seppala, I Hansen, T Salomaa, V Gharib, SA Eriksson, JG Bandinelli, S Pedersen, O Rich, SS Dedoussis, G Lehtimaki, T Ordovaas, JM AF Dashti, Hassan S. Follis, Jack L. Smith, Caren E. Tanaka, Toshiko Cade, Brian E. Gottlieb, Daniel J. Hruby, Adela Jacques, Paul F. Lamon-Fava, Stefania Richardson, Kris Saxena, Richa Scheer, Frank A. J. L. Kovanen, Leena Bartz, Traci M. Perala, Mia-Maria Jonsson, Anna Frazier-Wood, Alexis C. Kalafati, Ioanna-Panagiota Mikkila, Vera Partonen, Timo Lemaitre, Rozenn N. Lahti, Jari Hernandez, Dena G. Toft, Ulla Johnson, W. Craig Kanoni, Stavroula Raitakari, Olli T. Perola, Markus Psaty, Bruce M. Ferrucci, Luigi Grarup, Niels Highland, Heather M. Rallidis, Loukianos Kahonen, Mika Havulinna, Aki S. Siscovick, David S. Raikkonen, Katri Jorgensen, Torben Rotter, Jerome I. Deloukas, Panos Viikari, Jorma S. A. Mozaffarian, Dariush Linneberg, Allan Seppala, Ilkka Hansen, Torben Salomaa, Veikko Gharib, Sina A. Eriksson, Johan G. Bandinelli, Stefania Pedersen, Oluf Rich, Stephen S. Dedoussis, George Lehtimaki, Terho Ordovas, Jose M. TI Habitual sleep duration is associated with BMI and macronutrient intake and may be modified by CLOCK genetic variants SO AMERICAN JOURNAL OF CLINICAL NUTRITION LA English DT Article DE CLOCK; circadian rhythm; dietary intake; gene-environment interaction; sleep duration ID FOOD-FREQUENCY QUESTIONNAIRE; BODY-MASS INDEX; ENERGY-EXPENDITURE; METABOLIC SYNDROME; METAANALYSIS; ADULTS; OBESITY; SEX; CURTAILMENT; POPULATION AB Background: Short sleep duration has been associated with greater risks of obesity, hypertension, diabetes, and cardiovascular disease. Also, common genetic variants in the human Circadian Locomotor Output Cycles Kaput (CLOCK) show associations with ghrelin and total energy intake. Objectives: We examined associations between habitual sleep duration, body mass index (BM), and macronutrient intake and assessed whether CLOCK variants modify these associations. Design: We conducted inverse-variance weighted, fixed-effect meta-analyses of results of adjusted associations of sleep duration and BMI and macronutrient intake as percentages of total energy as well as interactions with CLOCK variants from 9 cohort studies including up to 14,906 participants of European descent from the Cohorts for Heart and Aging Research in Genomic Epidemiology Consortium. Results: We observed a significant association between sleep duration and lower BMI (beta +/- SE = 0.16 +/- 0.04, P < 0.0001) in the overall sample; however, associations between sleep duration and relative macronutrient intake were evident in age- and sex-stratified analyses only. We observed a significant association between sleep duration and lower saturated fatty acid intake in younger (aged 20-64 y) adults (men: 0.11 +/- 0.06%, P = 0.03; women: 0.10 +/- 0.05%, P = 0.04) and with lower carbohydrate (-0.31 0.12%, P < 0.01), higher total fat (0.18 +/- 0.09%, P = 0.05), and higher PUFA (0.05 +/- 0.02%, P = 0.02) intakes in older (aged 65-80 y) women. In addition, the following 2 nominally significant interactions were observed: between sleep duration and rs12649507 on PUFA intake and between sleep duration and rs6858749 on protein intake. Conclusions: Our results indicate that longer habitual sleep duration is associated with lower BMI and age- and sex-specific favorable dietary behaviors. Differences in the relative intake of specific macronutrients associated with short sleep duration could, at least in part, explain previously reported associations between short sleep duration and chronic metabolic abnormalities. In addition, the influence of obesity-associated CLOCK variants on the association between sleep duration and macronutrient intake suggests that longer habitual sleep duration could ameliorate the genetic predisposition to obesity via a favorable dietary profile. C1 Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Dashti, Hassan S.; Smith, Caren E.; Richardson, Kris; Ordovas, Jose M.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr & Genom Lab, Boston, MA 02111 USA. [Jacques, Paul F.] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr Epidemiol Lab, Boston, MA 02111 USA. [Lamon-Fava, Stefania] Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, Boston, MA 02111 USA. [Follis, Jack L.] Univ St Thomas, Dept Math Comp Sci & Cooperat Engn, Houston, TX 77006 USA. [Tanaka, Toshiko; Ferrucci, Luigi] NIH, Translat Gerontol Branch, Baltimore, MD USA. [Hernandez, Dena G.] NIH, Neurogenet Lab, Baltimore, MD USA. [Cade, Brian E.; Gottlieb, Daniel J.; Saxena, Richa; Scheer, Frank A. J. L.] Brigham & Womens Hosp, Div Sleep & Circadian Disorders, Boston, MA 02115 USA. [Mozaffarian, Dariush] Brigham & Womens Hosp, Div Cardiovasc Med, Boston, MA 02115 USA. [Mozaffarian, Dariush] Brigham & Womens Hosp, Channing Div Network Med, Boston, MA 02115 USA. [Cade, Brian E.; Gottlieb, Daniel J.; Scheer, Frank A. J. L.] Harvard Univ, Sch Med, Div Sleep Med, Boston, MA USA. [Mozaffarian, Dariush] Harvard Univ, Sch Med, Div Cardiovasc Med, Boston, MA USA. [Mozaffarian, Dariush] Harvard Univ, Sch Med, Charming Div Network Med, Boston, MA USA. [Gottlieb, Daniel J.] VA Boston Healthcare Syst, Sleep Disorders Ctr, Boston, MA USA. [Hruby, Adela; Mozaffarian, Dariush] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Saxena, Richa] Massachusetts Gen Hosp, Ctr Human Genet Res, Boston, MA 02114 USA. [Saxena, Richa] Massachusetts Gen Hosp, Dept Anesthesia Crit Care & Pain Med, Boston, MA 02114 USA. [Saxena, Richa] Harvard Univ, Sch Med, Boston, MA USA. [Kovanen, Leena; Partonen, Timo] Natl Inst Hlth & Welf, Dept Mental Hlth, Helsinki, Finland. [Kovanen, Leena; Partonen, Timo] Natl Inst Hlth & Welf, Dept Substance Abuse Serv, Helsinki, Finland. [Perala, Mia-Maria; Perola, Markus; Havulinna, Aki S.; Salomaa, Veikko; Eriksson, Johan G.] Natl Inst Hlth & Welf, Dept Chron Dis Prevent, Helsinki, Finland. [Lehtimaki, Terho] Natl Inst Hlth & Welf, Helsinki, Finland. [Bartz, Traci M.; Lemaitre, Rozenn N.; Psaty, Bruce M.] Univ Washington, Cardiovasc Hlth Res Unit, Seattle, WA 98195 USA. [Bartz, Traci M.; Lemaitre, Rozenn N.; Psaty, Bruce M.; Gharib, Sina A.] Univ Washington, Dept Med, Seattle, WA 98195 USA. [Bartz, Traci M.; Johnson, W. Craig] Univ Washington, Dept Biostat, Seattle, WA 98195 USA. [Psaty, Bruce M.] Univ Washington, Dept Epidemiol & Hlth Serv, Seattle, WA 98195 USA. [Gharib, Sina A.] Univ Washington, Dept Computat Med, Seattle, WA 98195 USA. [Gharib, Sina A.] Univ Washington, Ctr Lung Biol, Seattle, WA 98195 USA. [Jonsson, Anna; Grarup, Niels; Hansen, Torben; Pedersen, Oluf] Univ Copenhagen, Novo Nordisk Fdn, Ctr Basic Metab Res, Copenhagen, Denmark. [Linneberg, Allan] Univ Copenhagen, Dept Clin Med, Copenhagen, Denmark. Univ Copenhagen, Fac Hlth & Med Sci, Dept Clin Med, Copenhagen, Denmark. [Frazier-Wood, Alexis C.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Houston, TX 77030 USA. [Kalafati, Ioanna-Panagiota; Dedoussis, George] Harokopio Univ, Dept Nutr & Dietet, Athens, Greece. [Mikkila, Vera] Univ Helsinki, Dept Food & Environm Sci, Div Nutr, Helsinki, Finland. [Lahti, Jari; Raikkonen, Katri] Univ Helsinki, Inst Behav Sci, Helsinki, Finland. [Eriksson, Johan G.] Univ Helsinki, Dept Gen Practice & Primary Hlth Care, Helsinki, Finland. [Lahti, Jari; Eriksson, Johan G.] Folkhalsan Res Ctr, Helsinki, Finland. [Toft, Ulla; Jorgensen, Torben] Glostrup Univ Hosp, Res Ctr Prevent & Hlth, Glostrup, Denmark. [Linneberg, Allan] Glostrup Univ Hosp, Dept Clin Expt Res, Glostrup, Denmark. [Kanoni, Stavroula; Deloukas, Panos] William Harvey Res Inst, London, England. [Raitakari, Olli T.] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku, Finland. [Raitakari, Olli T.] Univ Turku, Dept Clin Physiol & Nucl Med, Turku, Finland. [Viikari, Jorma S. A.] Univ Turku, Dept Med, Turku, Finland. [Viikari, Jorma S. A.] Univ Turku, Div Med, Turku, Finland. [Viikari, Jorma S. A.] Turku Univ Hosp, FIN-20520 Turku, Finland. [Psaty, Bruce M.] Grp Hlth Res Inst, Grp Hlth, Seattle, WA USA. [Highland, Heather M.] Univ Texas Hlth Sci Ctr Houston, Ctr Human Genet, Grad Sch Biomed Sci Houston, Sch Publ Hlth, Houston, TX 77030 USA. [Rallidis, Loukianos] Univ Gen Hosp, Dept Cardiol, Athens, Greece. [Kahonen, Mika] Tampere Univ Hosp, Dept Clin Physiol, Tampere, Finland. [Kahonen, Mika] Univ Tampere, FIN-33101 Tampere, Finland. [Siscovick, David S.] New York Acad Med, New York, NY USA. [Rotter, Jerome I.] Harbor Univ Calif, Los Angeles Med Ctr, Los Angeles Biomed Res Inst, Inst Translat Genom & Populat Sci, Torrance, CA USA. [Deloukas, Panos] King Abdulaziz Univ, Princess Al Jawhara Al Brahim Ctr Excellence Res, Jeddah 21413, Saudi Arabia. [Seppala, Ilkka; Lehtimaki, Terho] Univ Tampere, Sch Med, Dept Clin Chem, Fimlab Labs, FIN-33101 Tampere, Finland. [Eriksson, Johan G.] Univ Helsinki, Cent Hosp, Unit Gen Practice, Helsinki, Finland. [Eriksson, Johan G.] Vasa Cent Hosp, Vaasa, Finland. [Bandinelli, Stefania] Azienda Sanit Firenze, Geriatr Unit, Florence, Italy. [Rich, Stephen S.] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA USA. [Ordovas, Jose M.] Ctr Nacl Invest Cardiovasc, Dept Epidemiol, Madrid, Spain. [Ordovas, Jose M.] Inst Madrilefio Estudios Avanzados Alimentac, Madrid, Spain. RP Ordovaas, JM (reprint author), Tufts Univ, Jean Mayer USDA, Human Nutr Res Ctr Aging, Nutr & Genom Lab, 711 Washington St, Boston, MA 02111 USA. EM jose.ordovas@tufts.edu RI Grarup, Niels/K-2807-2015; Deloukas, Panos/B-2922-2013; Wood, Lekki/B-8053-2010; OI Grarup, Niels/0000-0001-5526-1070; Linneberg, Allan/0000-0002-0994-0184; Eriksson, Johan/0000-0002-2516-2060; Raikkonen, Katri/0000-0003-3124-3470; Deloukas, Panos/0000-0001-9251-070X; Wood, Lekki/0000-0001-7616-2119; Kovanen, Leena/0000-0002-3552-124X; Partonen, Timo/0000-0003-1951-2455; Lahti, Jari/0000-0002-4310-5297; Jorgensen, Torben/0000-0001-9453-2830 FU USDA [58-1950-0-014]; National Heart, Lung, and Blood Institute (NELBI) [BL105756]; NIH [R21 DK089378, RO1 HL094806]; Academy of Finland [136635, 139635]; Finnish Foundation for Cardiovascular Research; NHLBI [HHSN268201200036C, HHSN268200800007C, N01HC55222, N01HC85079, N01HC85080, N01HC85081, N01HC85082, N01HC85083, N01HC85086]; NIMBI [HL080295, HL087652, HL105756, HL053916]; National Institute of Neurological Disorders and Stroke; National Institute on Aging [AG023629]; National Center for Advancing Translational Sciences; Clinical and Translational Science Institute (CTSI) [UL1TR000124]; National Institute of Diabetes and Digestive and Kidney Disease Diabetes Research Center [DK063491]; NHLBI' s Framingham Heart Study [N01-HC-25195]; Affymetrix Inc. [NO2HL-6-4278]; Academy of Finland; Finnish Diabetes Research Society; Folkhalsan Research Foundation; [K08 HL112845-01] FX Supported by the USDA under agreement 58-1950-0-014. The Infrastructure for the Cohorts for Heart and Aging Research in Genomic Epidemiology Consortium is supported in part by National Heart, Lung, and Blood Institute (NELBI) grant BL105756. CES is supported by K08 HL112845-01. FAJLS was supported in part by NIH grants R21 DK089378 and RO1 HL094806. The Corogene Controls study was supported by the Academy of Finland (136635 and 139635) and the Finnish Foundation for Cardiovascular Research. CHS (Cardiovascular Health Study) research was supported by NHLBI contracts HHSN268201200036C, HHSN268200800007C, N01HC55222, N01HC85079, N01HC85080, N01HC85081, N01HC85082, N01HC85083, and N01HC85086 and NIMBI grants HL080295, HL087652, HL105756, HL053916 with additional contribution from the National Institute of Neurological Disorders and Stroke. Additional support was provided through AG023629 from the National Institute on Aging. The provision of genotyping data were supported in part by the National Center for Advancing Translational Sciences, Clinical and Translational Science Institute (CTSI) grant UL1TR000124, and the National Institute of Diabetes and Digestive and Kidney Disease Diabetes Research Center grant DK063491 to the Southern California Diabetes Endocrinology Research Center. The Framingham Offspring Study was conducted in part using data and resources from the Framingham Heart Study of the NIMBI of the NIH and Boston University School of Medicine. This work was partially supported by the NHLBI' s Framingham Heart Study (contract N01-HC-25195) and its contract with Affymetrix Inc. for genotyping services (contract NO2HL-6-4278). The Helsinki Birth Cohort Study has been supported by grants from the Academy of Finland, the Finnish Diabetes Research Society, the Folkhalsan Research Foundation, the Novo Nordisk Foundation, Finska Lalcaresallskapet, the Signe and Ane Gyllenberg Foundation, the University of Helsinki, the Ministry of Education, the Aliokas Foundation, and the Emil Aaltonen Foundation. The Invecchiare in Chianti (Aging in the Chianti Area) study baseline (1998-2000) was supported as a "targeted project" (ICS110.1/ RF97.71) by the Italian Ministry of Health and in part by the U.S. National Institute on Aging (contracts 263 MD 9164 and 263 MD 821336). The Inter99 was financially supported by research grants from the Danish Research Council, the Danish Centre for Health Technology Assessment, Novo Nordisk Inc., the Research Foundation of Copenhagen County, the Ministry of Internal Affairs and Health, the Danish Heart Foundation, the Danish Pharmaceutical Association, the Augustinus Foundation; the lb Henriksen Foundation, the Becket Foundation, and the Danish Diabetes Association. Genetic studies were supported by The Lundbeck Foundation Centre for Applied Medical Genomics in Personalised Disease Prediction, Prevention and Care (www.lucamp.org). The Novo Nordisk Foundation Center for Basic Metabolic Researeh is an independent Research Center at the University of Copenhagen partially funded by an unrestricted donation from the Novo Nordisk Foundation (www.metabol.ku.dk). The Multi-Ethnic Study of Atherosclerosis (MESA) is conducted and supported by contracts NO1HC-95159, N01-HC-95160, N01-HC-95161, NO1-HC-95162, N01-HC95163, N01-HC-95164, N01-HC-95165, N01-HC-95166, N01-HC-95167, N01-HC-95168, NO1-HC-95169, and RR-024156 from the NHLBI. Funding for MESA SNP Health Association Resource (SHARe) genotyping was provided by NHLBI contract NO2-HL-6-4278.; For The Hellenic Study of Interactions between SNPs and Eating in Atherosclerosis Susceptibility study, PD is supported and funded by the National Institute for Health Research. The Cardiovascular.Risk in Young Finns Study has been financially supported by the Academy of Finland [grants 134309 (Eye), 126925, 121584, 124282, 129378 (Salve), 117787 (Gendi), and 41071 (Slcidi)], the Social Insurance Institution of Finland, Kuopio, Tampere and Turku University Hospital Medical Funds (grant 9M048 for 9NO35 for TeLeht), the Juho Vainio Foundation, the Paavo Nurmi Foundation, the Finnish Foundation of Cardiovascular Research and Finnish Cultural Foundation, the Tampere Tuberculosis Foundation, and the Emil Aaltonen Foundation. NR 40 TC 21 Z9 21 U1 3 U2 36 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0002-9165 EI 1938-3207 J9 AM J CLIN NUTR JI Am. J. Clin. Nutr. PD JAN PY 2015 VL 101 IS 1 BP 135 EP 143 DI 10.3945/ajcn.114.095026 PG 9 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AX4ID UT WOS:000346895700016 PM 25527757 ER PT J AU Meikle, WG Holst, N AF Meikle, W. G. Holst, N. TI Application of continuous monitoring of honeybee colonies SO APIDOLOGIE LA English DT Review DE continuous hive weight; colony temperature; colony humidity; forager traffic; hive vibration ID APIS-MELLIFERA COLONIES; BEES HYMENOPTERA; FLIGHT ACTIVITY; SYSTEMIC INSECTICIDES; HIVE; BEHAVIOR; APIDAE; NEST; THERMOREGULATION; TEMPERATURE AB Monitoring physical variables associated with honeybee colonies, including weight, temperature, humidity, respiratory gases, vibration, sound, and forager traffic, in a continuous manner is becoming feasible for most researchers as the cost and size of electronic sensors decrease while their precision and capacity increase. Researchers have taken different approaches to collecting and analyzing the resulting datasets, with a view toward extracting information on colony behavior and phenology. The objective of this review is to examine critically the different kinds of data and data analyses, providing researchers with better-informed options for obtaining information on colony phenology in the field without disturbing the hive, and for combining information from different kinds of sensors to obtain a more complete picture of colony status. Wireless sensor networks and powering sensors are briefly discussed. C1 [Meikle, W. G.] ARS, USDA, Tucson, AZ 85719 USA. [Holst, N.] Aarhus Univ, Dept Agroecol, DK-4200 Slagelse, Denmark. RP Meikle, WG (reprint author), ARS, USDA, 2000 E Allen Rd, Tucson, AZ 85719 USA. EM william.meikle@ars.usda.gov NR 63 TC 7 Z9 7 U1 4 U2 37 PU SPRINGER FRANCE PI PARIS PA 22 RUE DE PALESTRO, PARIS, 75002, FRANCE SN 0044-8435 EI 1297-9678 J9 APIDOLOGIE JI Apidologie PD JAN PY 2015 VL 46 IS 1 BP 10 EP 22 DI 10.1007/s13592-014-0298-x PG 13 WC Entomology SC Entomology GA AX3SJ UT WOS:000346858800002 ER PT J AU Hales, K Parker, DB Cole, NA AF Hales, Kristin Parker, David B. Cole, N. Andy TI Volatile organic compound flux from manure of cattle fed diets differing in grain processing method and co-product inclusion SO ATMOSPHERIC ENVIRONMENT LA English DT Article DE Odor; Cattle; Manure; Volatile organic compound ID WET DISTILLERS GRAINS; IN-VITRO FERMENTATION; STEAM-FLAKED CORN; ODOR; SOLUBLES; ORIGIN; COMPONENTS; EMISSIONS; EFFLUENT; BALANCE AB Odor emissions from livestock production have become increasingly important in the past decade. Odors derived from animal feeding operations are caused by odorous VOC emitted from the mixture of feces and urine, as well as feed and silage which may be experiencing microbial fermentation. Distillers grains are a by-product of corn grain fermentation used to produce fuel ethanol, and this industry has grown rapidly throughout the U.S. in past years. Therefore, the use of wet distillers grains with solubles (WDGS) in feedlot cattle diets has also increased. The objective of this research was to determine specific VOC emissions from feces and urine or a mixture of both, from cattle fed steam flaked or dry-rolled corn (DRC)-based diets containing either 0% or 30% WDGS. Flux of dimethyl trisulfide was greater from feces of cattle fed DRC than steam-flaked corn (SFC) diets. No other differences in flux from feces were detected across dietary treatments for phenol, 4-methylphenol, indole, skatole, dimethyl disulfide, and flux of volatile fatty acids (VFA) such as acetic, propionic, isobutyric, butyric, isovaleric, and valeric acids (P > 0.15). Flux of skatole, acetic acid, and valeric acid from urine was greater for cattle fed SFC than DRC diets (P < 0.05). Moreover, dimethyl disulfide flux was greater for cattle fed DRC vs. SFC diets (P = 0.05). When evaluating WDGS inclusion in the diet, flux of acetic acid and heptanoic acid from urine was greater when cattle were fed diets containing 0% WDGS than 30% WDGS (P < 0.05). When combining urine and feces in the ratio in which they were excreted from the animal, flux of propionic acid was greater when cattle were fed DRC vs. SFC diets (P = 0.05). Based on these results, the majority of the VOC, VFA, and odor flux from cattle feeding operations is from the urine. Therefore, dietary strategies to reduce odor from cattle feeding facilities should primarily focus on reducing excretion of odorous compounds in the urine. Published by Elsevier Ltd. C1 [Hales, Kristin; Parker, David B.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. [Cole, N. Andy] USDA ARS, Conservat & Prod Res Lab, Bushland, TX 79012 USA. RP Hales, K (reprint author), USDA ARS, US Meat Anim Res Ctr, POB 166, Clay Ctr, NE 68933 USA. EM Kristin.Hales@ars.usda.gov; Dparker@mail.wtamu.edu; Andy.Cole@ars.usda.gov NR 18 TC 0 Z9 0 U1 4 U2 18 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 1352-2310 EI 1873-2844 J9 ATMOS ENVIRON JI Atmos. Environ. PD JAN PY 2015 VL 100 BP 20 EP 24 DI 10.1016/j.atmosenv.2014.10.037 PG 5 WC Environmental Sciences; Meteorology & Atmospheric Sciences SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences GA AY0CZ UT WOS:000347266700003 ER PT J AU Tomlinson, PJ Savin, MC Moore, PA AF Tomlinson, Peter J. Savin, Mary C. Moore, Philip A., Jr. TI Long-term applications of untreated and alum-treated poultry litter drive soil nitrogen concentrations and associated microbial community dynamics SO BIOLOGY AND FERTILITY OF SOILS LA English DT Article DE Alum; Poultry litter; Microorganism; Ammonia oxidizer; Glucosaminidase; Inorganic N ID AMMONIA-OXIDIZING BACTERIA; BETA-GLUCOSAMINIDASE ACTIVITY; REDUCING PHOSPHORUS RUNOFF; CHEMICAL AMENDMENTS; PHOSPHATASE-ACTIVITIES; ENZYME-ACTIVITIES; DAIRY MANURE; BIOMASS-C; ARCHAEA; DIVERSITY AB Aluminum sulfate (alum) treatment retains ammonia in poultry litter, potentially altering nitrogen (N) cycling after application to soil. The objective of this research was to assess if 8 and 9 years of annual application of untreated or alum-treated poultry litters or ammonium nitrate have resulted in detectable differences in N concentrations and cycling in soil. Soil N concentrations, beta-glucosaminidase activity, and ammonia oxidizer community richness were measured prior to, 10 days, and 1 and 6 months following application in years 8 and 9 in soil receiving either 2.24 (low rate) or 8.96 Mg litter ha(-1) (high rate) or 65 or 260 kg N ha(-1) (approximately equivalent N rates) of ammonium nitrate. There were few effects when fertilizers were applied at the low rate, but soil N did increase in all treatments relative to the control. Soil N, microbial biomass N, and beta-glucosaminidase activity were significantly higher in the alum-treated and untreated poultry litter when applied at the high rate compared to other treatments. Soil inorganic N increased significantly 10 days following application with the high rate of alum-treated litter and ammonium nitrate but not with untreated poultry litter. Ammonia oxidizer community compositions in the high rate of alum-treated and untreated poultry litter treatments were similar to each other and more closely related to the community in the ammonium nitrate treatment than the control. All fertilizers applied at the high rate altered the nitrifier community richness; however, the inputs of both poultry litters increased microbial biomass and enzyme activity, indicators of soil quality. C1 [Tomlinson, Peter J.; Savin, Mary C.] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72701 USA. [Moore, Philip A., Jr.] USDA ARS, Fayetteville, AR 72701 USA. RP Savin, MC (reprint author), Univ Arkansas, Dept Crop Soil & Environm Sci, 115 Plant Sci Bldg, Fayetteville, AR 72701 USA. EM ptomlin@k-state.edu; msavin@uark.edu FU Southern Region-Sustainable Agriculture Research and Education Program Graduate Student Grant [GS03-030]; Arkansas Agricultural Experiment Station, Research Incentive Program; Water Resources Research Institute program through the USGS [2003AR47B] FX This research was supported in part by a Southern Region-Sustainable Agriculture Research and Education Program Graduate Student Grant, project #: GS03-030; Arkansas Agricultural Experiment Station, Research Incentive Program; and the Water Resources Research Institute program through the USGS, project #: 2003AR47B. The authors would like to thank Jerry Martin, Scott Becton, Kim Payne, and all other laboratory and support personnel who helped with this project. NR 59 TC 2 Z9 2 U1 4 U2 32 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0178-2762 EI 1432-0789 J9 BIOL FERT SOILS JI Biol. Fertil. Soils PD JAN PY 2015 VL 51 IS 1 BP 43 EP 55 DI 10.1007/s00374-014-0949-0 PG 13 WC Soil Science SC Agriculture GA AX8GU UT WOS:000347148900005 ER PT J AU Zhai, LM Caiji, ZM Liu, J Wang, HY Ren, TZ Gai, XP Xi, B Liu, HB AF Zhai, Limei CaiJi, Zhuoma Liu, Jian Wang, Hongyuan Ren, Tianzhi Gai, Xiapu Xi, Bin Liu, Hongbin TI Short-term effects of maize residue biochar on phosphorus availability in two soils with different phosphorus sorption capacities SO BIOLOGY AND FERTILITY OF SOILS LA English DT Article DE Biochar; Fluvo-aquic soil; Phosphorus availability; Phosphorus sorption capacity; Red earth; Soil Olsen-P ID MICROBIAL BIOMASS; RELEASE; WATER; FRACTIONATION; COMBUSTION; DESORPTION; PHOSPHATE; QUALITY; RUNOFF AB This study investigated the effects of maize (Zea mays L.) straw biochar on phosphorus (P) availability in two soils with different P sorption capacities (iron and aluminum dominated slight acid Red earth and calcium dominated alkaline Fluvo-aquic soil). A 42-day incubation experiment was conducted to study how applications of biochar at different rates (0, 2, 4, and 8 % soil, w/w), in combination with and without mineral KH2PO4 fertilizer, affected contents of soil Olsen-P and soil microbial biomass P (SMB-P) and phosphomonoesterase activity. In addition, P sorption characteristics of soils amended with biochar, as well as main properties of the biochar and the soils, were determined. Application of 8 % biochar after 42 days of incubation substantially increased soil Olsen-P from 3 to 46 mg kg(-1) in Red earth and from 13 to 137 mg kg(-1) in Fluvo-aquic soil and increased SMB-P from 1 to 9 mg kg(-1) in Red earth and from 9 to 21 mg kg(-1) in Fluvo-aquic soil. The increase was mainly due to high concentrations of P in the ash fraction (77 % of total biochar P). Biochar effect on soil Olsen-P and SMB-P increased by higher biochar application rates and by lower P sorption capacity. Biochar application significantly reduced acid phosphomonoesterase activity in Red earth and alkaline phosphomonoesterase activity in Fluvo-aquic soil due to large amount of inorganic P added. We conclude that maize straw biochar is promising to potentially improve soil P availability in low-P soils, but further research at field scale is needed to confirm this. C1 [Zhai, Limei; CaiJi, Zhuoma; Liu, Jian; Wang, Hongyuan; Gai, Xiapu; Xi, Bin; Liu, Hongbin] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Nonpoint Source Pollut Control, Beijing 100081, Peoples R China. [CaiJi, Zhuoma] Minzu Univ China, Coll Life & Environm Sci, Beijing 100086, Peoples R China. [Liu, Jian] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. [Ren, Tianzhi] Minist Agr, Inst Agroenvironm Protect, Tianjin 300191, Peoples R China. RP Liu, HB (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Nonpoint Source Pollut Control, Beijing 100081, Peoples R China. EM liuhongbin@caas.cn RI Liu, Jian/P-4050-2014 OI Liu, Jian/0000-0003-4199-1296 FU National Natural Science Foundation of China [41203072]; Special Fund for Agro-scientific Research in the Public Interest [201303095-10] FX This study was funded by the National Natural Science Foundation of China (41203072) and the Special Fund for Agro-scientific Research in the Public Interest (201303095-10). NR 52 TC 3 Z9 8 U1 11 U2 121 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0178-2762 EI 1432-0789 J9 BIOL FERT SOILS JI Biol. Fertil. Soils PD JAN PY 2015 VL 51 IS 1 BP 113 EP 122 DI 10.1007/s00374-014-0954-3 PG 10 WC Soil Science SC Agriculture GA AX8GU UT WOS:000347148900011 ER PT J AU Zhang, JE Yu, JY Ouyang, Y AF Zhang, Jia-En Yu, Jiayu Ouyang, Ying TI Activity of Earthworm in Latosol Under Simulated Acid Rain Stress SO BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY LA English DT Article DE Acid rain; Enzyme activity; Earthworm; Latosol ID SOUTH CHINA; DEPOSITION AB Acid rain is still an issue of environmental concerns. This study investigated the impacts of simulated acid rain (SAR) upon earthworm activity from the Latosol (acidic red soil). Laboratory experiment was performed by leaching the soil columns grown with earthworms (Eisenia fetida) at the SAR pH levels ranged from 2.0 to 6.5 over a 34-day period. Results showed that earthworms tended to escape from the soil and eventually died for the SAR at pH = 2.0 as a result of acid toxicity. The catalase activity in the earthworms decreased with the SAR pH levels, whereas the superoxide dismutases activity in the earthworms showed a fluctuate pattern: decreasing from pH 6.5 to 5.0 and increasing from pH 5.0 to 4.0. Results implied that the growth of earthworms was retarded at the SAR pH a parts per thousand currency sign 3.0. C1 [Zhang, Jia-En; Yu, Jiayu] South China Agr Univ, Dept Ecol, Guangzhou, Guangdong, Peoples R China. [Ouyang, Ying] US Forest Serv, USDA, Mississippi State, MS 39762 USA. RP Zhang, JE (reprint author), South China Agr Univ, Dept Ecol, Wushan Rd, Guangzhou, Guangdong, Peoples R China. EM jeanzh@scau.edu.cn FU National Natural Science Foundation of China [40871118]; Natural Science Foundation of Guangdong Province, China [8151064201000048, 9451064201003801, S2011010001570]; China Postdoctoral Special Fund [201003355] FX The study was supported by National Natural Science Foundation of China (No. 40871118), Natural Science Foundation of Guangdong Province, China (No. 8151064201000048; No. 9451064201003801; and No. S2011010001570), and China Postdoctoral Special Fund (No. 201003355). NR 23 TC 2 Z9 2 U1 9 U2 29 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0007-4861 EI 1432-0800 J9 B ENVIRON CONTAM TOX JI Bull. Environ. Contam. Toxicol. PD JAN PY 2015 VL 94 IS 1 BP 108 EP 111 DI 10.1007/s00128-014-1404-5 PG 4 WC Environmental Sciences; Toxicology SC Environmental Sciences & Ecology; Toxicology GA AX9UU UT WOS:000347248300019 PM 25351717 ER PT J AU Zwollo, P Ray, JC Sestito, M Kiernan, E Wiens, GD Kaattari, S StJacques, B Epp, L AF Zwollo, Patty Ray, Jocelyn C. Sestito, Michael Kiernan, Elizabeth Wiens, Gregory D. Kaattari, Steve StJacques, Brittany Epp, Lidia TI B cell signatures of BCWD-resistant and susceptible lines of rainbow trout: A shift towards more EBF-expressing progenitors and fewer mature B cells in resistant animals SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE B lymphoid development; Humoral immunity; Rainbow trout; Selective breeding; Disease resistance; Flavobacterium psychrophilum ID COLD-WATER DISEASE; ONCORHYNCHUS-MYKISS; FLAVOBACTERIUM-PSYCHROPHILUM; IMMUNE TISSUES; SALMONID FISH; TELEOST FISH; BONE-MARROW; POPULATIONS; GENE; KIDNEY AB Bacterial cold water disease (BCWD) is a chronic disease of rainbow trout, and is caused by the Gram-negative bacterium Flavobacterium psychrophilum (Fp), a common aquaculture pathogen. The National Center for Cool and Cold Water Aquaculture has bred two genetic lines of rainbow trout: a line of Fp-resistant trout (ARS-Fp-R or R-line trout) and a line of susceptible trout (ARS-Fp-S, or S-line). Little is known about how phenotypic selection alters immune response parameters or how such changes relate to genetic disease resistance. Herein, we quantify interindividual variation in the distribution and abundance of B cell populations (B cell signatures) and examine differences between genetic lines of naive animals. There are limited trout-specific cell surface markers currently available to resolve B cell sub-populations and thus we developed an alternative approach based on detection of differentially expressed transcription factors and intracellular cytokines. B cell signatures were compared between R-line and S-line trout by flow cytometry using antibodies against transcription factors early B cell factor-1 (EBF1) and paired domain box protein Pax5, the pro-inflammatory cytokine IL-1 beta, and the immunoglobulin heavy chain mu. R-line trout had higher percentages of EBF+ B myeloid/progenitor and pre-B cells in PBL, anterior and posterior kidney tissues compared to S-line trout. The opposite pattern was detected in more mature B cell populations: R-line trout had lower percentages of both IgM(+) mature B cells and IgM-secreting cells in anterior kidney and PBL compared to S-line trout. In vitro LPS-activation studies of PBL and spleen cell cultures revealed no significant induction differences between R-line and S-line trout. Together, our findings suggest that selective resistance to BCWD may be associated with shifts in naive animal developmental lineage commitment that result in decreased B lymphopoiesis and increased myelopoiesis in BCWD resistant trout relative to susceptible trout. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Zwollo, Patty; Ray, Jocelyn C.; Sestito, Michael; Kiernan, Elizabeth; StJacques, Brittany; Epp, Lidia] Coll William & Mary, Dept Biol, Williamsburg, VA 23185 USA. [Kaattari, Steve] Coll William & Mary, Virginia Inst Marine Sci, Dept Environm & Aquat Anim Hlth, Williamsburg, VA 23185 USA. [Wiens, Gregory D.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Zwollo, P (reprint author), Coll William & Mary, Dept Biol, Williamsburg, VA 23185 USA. EM pxzwol@wm.edu OI Wiens, Gregory/0000-0002-5478-7780 FU USDA-NIFA [2012-67015-30217]; Department of Energy [DE-FG09-93ER-20097] FX We thank Dr. Timothy Leeds (NCCCWA) for breeding and providing R- and S-line fish utilized in these experiments. The authors wish to thank William and Mary undergraduate students from Immunology lab course BIO438 (Spring 2012, Spring 2014) for help with important in vitro pilot experiments involving LPS-induction of immune tissues from both trout lines. This study was funded through USDA-NIFA grant No. 2012-67015-30217. Carbohydrate analysis performed by Dr. Parastoo Azadi at the University of Georgia is supported in part by the Department of Energy-funded (DE-FG09-93ER-20097) Center for Plant and Microbial Complex Carbohydrates. NR 48 TC 0 Z9 0 U1 1 U2 12 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD JAN PY 2015 VL 48 IS 1 BP 1 EP 12 DI 10.1016/j.dci.2014.07.018 PG 12 WC Immunology; Zoology SC Immunology; Zoology GA AY0BR UT WOS:000347263500001 PM 25101978 ER PT J AU Jeong, J Kim, WH Fernandez, CP Kim, S Kim, YH Jang, HK Lillehoj, HS Woo, HJ Min, W AF Jeong, Jipseol Kim, Woo H. Fernandez, Cherry P. Kim, Suk Kim, Yong-Hwan Jang, Hyung-Kwan Lillehoj, Hyun S. Woo, Hee-Jong Min, Wongi TI Different strategies for producing naturally soluble form of common cytokine receptor gamma chain SO DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY LA English DT Article DE Common cytokine receptor gamma chain; gamma(c); Soluble gamma(c); Ectodomain shedding; Shedding mechanisms ID SEVERE COMBINED IMMUNODEFICIENCY; CELL-LINES; DISTINCT PHENOTYPES; BIOLOGICAL FUNCTION; T-CELLS; INTERLEUKIN-2-RECEPTOR; GENERATION; INSIGHTS; DISEASE; BETA AB The common cytokine receptor gamma chain (gamma(c)) plays an essential role in regulating lymphoid homeostasis. In fact, alteration of this gene causes severe immunodeficiency in humans and animals. Although soluble gamma(c) (s gamma(c)) was identified in the late 1990s, much remains unknown about its production. This study describes various mechanisms underlying the generation of s gamma(c) isoforms in different species. Our data demonstrate that mouse gamma(c) and the avian ortholog gamma(c)-a did not generate s gamma(c). Moreover, two mouse isoforms, CRA-a and m gamma(c)-b, encoded by transcripts lacking a transmembrane region by alternative splicing, did not yield s gamma(c). However, in ducks, s gamma(c) was produced from a gamma(c)-b transcript lacking a transmembrane region by alternative splicing. In chickens, s gamma(c) was produced in normal cells and cell lines by proteolytic shedding of the gamma(c)-b isoform containing intron 5, which displayed a relatively high probability of proteolytic cleavage of the ectodomain. This shedding was suppressed by leupeptin, serine and cysteine protease inhibitor. Compared to the chicken ortholog gamma(c)-a, expression of gamma(c)-b mRNA was differentially regulated according to tissue type, developmental stage, and antigen stimulation. These data demonstrate several mechanisms for producing s gamma(c) and suggest a potential role for s gamma(c) in avian lymphoid homeostatic responses to environmental antigens. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Jeong, Jipseol; Kim, Woo H.; Fernandez, Cherry P.; Kim, Suk; Kim, Yong-Hwan; Min, Wongi] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, South Korea. [Jeong, Jipseol; Kim, Woo H.; Fernandez, Cherry P.; Kim, Suk; Kim, Yong-Hwan; Min, Wongi] Gyeongsang Natl Univ, Inst Anim Med, Jinju 660701, South Korea. [Jang, Hyung-Kwan] Chonbuk Natl Univ, Coll Vet Med, Dept Infect Dis, Jeonju 561756, South Korea. [Jang, Hyung-Kwan] Chonbuk Natl Univ, Coll Vet Med, Dept Avian Dis, Jeonju 561756, South Korea. [Jang, Hyung-Kwan] Chonbuk Natl Univ, Korea Zoonosis Res Inst, Jeonju 561756, South Korea. [Lillehoj, Hyun S.] USDA ARS, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. [Woo, Hee-Jong] Seoul Natl Univ, Coll Vet Med, Immunol Lab, Seoul 151742, South Korea. RP Min, W (reprint author), Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, South Korea. EM wongimin@gnu.ac.kr FU WCU program [R33-10013]; Basic Science Research Program [NRF-2013R1A1A4A01006646]; Cooperative Research Program for Agriculture Science & Technology Development, RDA, Republic of Korea [PJ907105032013] FX This research was supported by the WCU program (R33-10013), by Basic Science Research Program (NRF-2013R1A1A4A01006646), and by the Cooperative Research Program for Agriculture Science & Technology Development (PJ907105032013), RDA, Republic of Korea. NR 44 TC 1 Z9 1 U1 0 U2 2 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0145-305X EI 1879-0089 J9 DEV COMP IMMUNOL JI Dev. Comp. Immunol. PD JAN PY 2015 VL 48 IS 1 BP 13 EP 21 DI 10.1016/j.dci.2014.08.008 PG 9 WC Immunology; Zoology SC Immunology; Zoology GA AY0BR UT WOS:000347263500002 PM 25173813 ER PT J AU Fleming, AL Wang, GX McRoberts, RE AF Fleming, Andrew L. Wang, Guangxing McRoberts, Ronald E. TI Comparison of methods toward multi-scale forest carbon mapping and spatial uncertainty analysis: combining national forest inventory plot data and landsat TM images SO EUROPEAN JOURNAL OF FOREST RESEARCH LA English DT Article DE Co-simulation; Forest inventory plot; Forest carbon mapping; Landsat TM image; Scale up; Spatial uncertainty ID GROWING STOCK VOLUME; WOODY BIOMASS; CANADA; SINKS; SEQUESTRATION; VEGETATION; LOCATIONS; STORAGE; CHINA; MODEL AB Accurate spatial estimation of forest carbon stocks and their spatial uncertainties at local, regional, national, and global scales is a critical step in global carbon cycle modeling and management. This study aimed at enhancing the methods that are currently used in this area by combining plot data from the forest inventory and analysis program of the U.S. Forest Service and free landsat thematic mapper image data. Three mapping methods including linear regression, sequential Gaussian co-simulation, and block co-simulation algorithm were compared with respect to the accuracy of forest carbon stock estimates obtained for a study area in Southern Illinois, USA. The results indicated that although the linear regression resulted in smaller prediction errors than the sequential Gaussian co-simulation and the block co-simulation approaches, it also produced both negative and unreasonably large estimates, which is a serious drawback. Moreover, the sequential Gaussian co-simulation and the block co-simulation produced not only accurate carbon predictions, but also uncertainties for the local estimates. In addition, the block co-simulation approach scaled up both forest carbon stocks and the input uncertainties from finer to coarser spatial resolutions as is required for mapping forest carbon at national and global scales. Thus, the co-simulation and block co-simulation algorithms resolved an important current methodological challenge. C1 [Fleming, Andrew L.; Wang, Guangxing] So Illinois Univ, Dept Geog & Environm Resources, Carbondale, IL 62901 USA. [McRoberts, Ronald E.] US Forest Serv, No Res Stn, St Paul, MN 55108 USA. RP Wang, GX (reprint author), So Illinois Univ, Dept Geog & Environm Resources, 1000 Faner Dr, Carbondale, IL 62901 USA. EM gxwang@siu.edu FU Southern Illinois University Carbondale FX We thank the Northern Research Station, U.S. Forest Service, for providing the FIA data, Southern Illinois University Carbondale for the project funding, and two undergraduate students (Franklin Wesseln and Adam Bauer) for collection of data for the additional sample plots. NR 34 TC 4 Z9 4 U1 0 U2 21 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1612-4669 EI 1612-4677 J9 EUR J FOREST RES JI Eur. J. For. Res. PD JAN PY 2015 VL 134 IS 1 BP 125 EP 137 DI 10.1007/s10342-014-0838-y PG 13 WC Forestry SC Forestry GA AY0JH UT WOS:000347282500010 ER PT J AU Nowacki, GJ Abrams, MD AF Nowacki, Gregory J. Abrams, Marc D. TI Is climate an important driver of post-European vegetation change in the Eastern United States? SO GLOBAL CHANGE BIOLOGY LA English DT Review DE altered disturbance regimes; climate change; conifer-northern hardwoods; land use; mesophication; oak-pine forests; subboreal forests ID LAND-USE HISTORY; HEMLOCK-HARDWOOD FORESTS; GREAT-LAKES REGION; WESTERN NEW-YORK; PRESETTLEMENT FORESTS; NEW-ENGLAND; CENTRAL PENNSYLVANIA; DISTURBANCE REGIMES; LANDSCAPE STRUCTURE; NORTHERN MINNESOTA AB Many ecological phenomena combine to direct vegetation trends over time, with climate and disturbance playing prominent roles. To help decipher their relative importance during Euro-American times, we employed a unique approach whereby tree species/genera were partitioned into temperature, shade tolerance, and pyrogenicity classes and applied to comparative tree-census data. Our megadata analysis of 190 datasets determined the relative impacts of climate vs. altered disturbance regimes for various biomes across the eastern United States. As the Euro-American period (ca. 1500 to today) spans two major climatic periods, from Little Ice Age to the Anthropocene, vegetation changes consistent with warming were expected. In most cases, however, European disturbance overrode regional climate, but in a manner that varied across the Tension Zone Line. To the north, intensive and expansive early European disturbance resulted in the ubiquitous loss of conifers and large increases of Acer, Populus, and Quercus in northern hardwoods, whereas to the south, these disturbances perpetuated the dominance of Quercus in central hardwoods. Acer increases and associated mesophication in Quercus-Pinus systems were delayed until mid 20th century fire suppression. This led to significant warm to cool shifts in temperature class where cool-adapted Acer saccharum increased and temperature neutral changes where warm-adapted Acer rubrum increased. In both cases, these shifts were attributed to fire suppression rather than climate change. Because mesophication is ongoing, eastern US forests formed during the catastrophic disturbance era followed by fire suppression will remain in climate disequilibrium into the foreseeable future. Overall, the results of our study suggest that altered disturbance regimes rather than climate had the greatest influence on vegetation composition and dynamics in the eastern United States over multiple centuries. Land-use change often trumped or negated the impacts of warming climate, and needs greater recognition in climate change discussions, scenarios, and model interpretations. C1 [Nowacki, Gregory J.] US Forest Serv, Eastern Reg Off, USDA, Milwaukee, WI 53202 USA. [Abrams, Marc D.] Penn State Univ, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. RP Nowacki, GJ (reprint author), US Forest Serv, Eastern Reg Off, USDA, 626 E Wisconsin Ave, Milwaukee, WI 53202 USA. EM gnowacki@fs.fed.us NR 169 TC 38 Z9 38 U1 15 U2 86 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JAN PY 2015 VL 21 IS 1 BP 314 EP 334 DI 10.1111/gcb.12663 PG 21 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX1HJ UT WOS:000346698100029 PM 24953341 ER PT J AU Wilcox, KR von Fischer, JC Muscha, JM Petersen, MK Knapp, AK AF Wilcox, Kevin R. von Fischer, Joseph C. Muscha, Jennifer M. Petersen, Mark K. Knapp, Alan K. TI Contrasting above- and belowground sensitivity of three Great Plains grasslands to altered rainfall regimes SO GLOBAL CHANGE BIOLOGY LA English DT Article DE aboveground productivity; belowground productivity; carbon cycling; climate change; ecosystem function; ecosystem services; grassland; precipitation amount; precipitation pattern ID NET PRIMARY PRODUCTION; PRIMARY PRODUCTIVITY; USE EFFICIENCY; CLIMATE-CHANGE; PRECIPITATION VARIABILITY; TALLGRASS PRAIRIE; GLOBAL CHANGE; ECOSYSTEM; DYNAMICS; NORTHERN AB Intensification of the global hydrological cycle with atmospheric warming is expected to increase interannual variation in precipitation amount and the frequency of extreme precipitation events. Although studies in grasslands have shown sensitivity of aboveground net primary productivity (ANPP) to both precipitation amount and event size, we lack equivalent knowledge for responses of belowground net primary productivity (BNPP) and NPP. We conducted a 2-year experiment in three US Great Plains grasslands - the C-4-dominated shortgrass prairie (SGP; low ANPP) and tallgrass prairie (TGP; high ANPP), and the C-3-dominated northern mixed grass prairie (NMP; intermediate ANPP) - to test three predictions: (i) both ANPP and BNPP responses to increased precipitation amount would vary inversely with mean annual precipitation (MAP) and site productivity; (ii) increased numbers of extreme rainfall events during high-rainfall years would affect high and low MAP sites differently; and (iii) responses belowground would mirror those aboveground. We increased growing season precipitation by as much as 50% by augmenting natural rainfall via (i) many (11-13) small or (ii) fewer (3-5) large watering events, with the latter coinciding with naturally occurring large storms. Both ANPP and BNPP increased with water addition in the two C-4 grasslands, with greater ANPP sensitivity in TGP, but greater BNPP and NPP sensitivity in SGP. ANPP and BNPP did not respond to any rainfall manipulations in the C-3-dominated NMP. Consistent with previous studies, fewer larger (extreme) rainfall events increased ANPP relative to many small events in SGP, but event size had no effect in TGP. Neither system responded consistently above- and belowground to event size; consequently, total NPP was insensitive to event size. The diversity of responses observed in these three grassland types underscores the challenge of predicting responses relevant to C cycling to forecast changes in precipitation regimes even within relatively homogeneous biomes such as grasslands. C1 [Wilcox, Kevin R.; von Fischer, Joseph C.; Knapp, Alan K.] Colorado State Univ, Dept Biol, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Muscha, Jennifer M.; Petersen, Mark K.] ARS, Ft Keogh Livestock & Range Res Lab, USDA, Miles City, MT 59301 USA. RP Wilcox, KR (reprint author), Colorado State Univ, Dept Biol, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. EM Kevin.wilcox@colostate.edu OI von Fischer, Joseph/0000-0001-5508-6073 FU Konza Prairie Long-Term Ecological Research program FX This research was supported by the Konza Prairie Long-Term Ecological Research program. We would like to thank E. Rosenlieb for help administering treatments, K. Byrne for sharing expertise concerning BNPP sampling techniques, K. Reinhart for sharing soil bulk density measurements for our site in NMP, P. O'Neal, J. Taylor, M. Ashby, J. Thomas and other staff at the Konza Prairie Biological Station, Central Plains Experimental Range, and Fort Keogh Livestock and Range Research Laboratory for site-based assistance, and M. Avolio, D. Hoover, S. Koerner, and K. La Pierre for assistance in the field. Lastly, we would like to thank three anonymous reviewers for their comments, which greatly improved the quality and clarity of this manuscript. NR 60 TC 19 Z9 20 U1 14 U2 132 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JAN PY 2015 VL 21 IS 1 BP 335 EP 344 DI 10.1111/gcb.12673 PG 10 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX1HJ UT WOS:000346698100030 PM 25044242 ER PT J AU Linquist, BA Anders, MM Adviento-Borbe, MAA Chaney, RL Nalley, LL Da Rosa, EFF Van Kessel, C AF Linquist, Bruce A. Anders, Merle M. Adviento-Borbe, Maria Arlene A. Chaney, Rufus L. Nalley, L. Lanier Da Rosa, Eliete F. F. Van Kessel, Chris TI Reducing greenhouse gas emissions, water use, and grain arsenic levels in rice systems SO GLOBAL CHANGE BIOLOGY LA English DT Article DE alternate wetting and drying; arsenic; greenhouse gas emissions; irrigation management; Oryza sativa; sustainable intensification; water-use efficiency ID METHANE EMISSION; AGRICULTURAL INTENSIFICATION; CARBON SEQUESTRATION; DRYING IRRIGATION; CROP PRODUCTION; ORGANIC-CARBON; CLIMATE-CHANGE; N2O EMISSIONS; PADDY FIELDS; MANAGEMENT AB Agriculture is faced with the challenge of providing healthy food for a growing population at minimal environmental cost. Rice (Oryza sativa), the staple crop for the largest number of people on earth, is grown under flooded soil conditions and uses more water and has higher greenhouse gas (GHG) emissions than most crops. The objective of this study was to test the hypothesis that alternate wetting and drying (AWD - flooding the soil and then allowing to dry down before being reflooded) water management practices will maintain grain yields and concurrently reduce water use, greenhouse gas emissions and arsenic (As) levels in rice. Various treatments ranging in frequency and duration of AWD practices were evaluated at three locations over 2years. Relative to the flooded control treatment and depending on the AWD treatment, yields were reduced by <1-13%; water-use efficiency was improved by 18-63%, global warming potential (GWP of CH4 and N2O emissions) reduced by 45-90%, and grain As concentrations reduced by up to 64%. In general, as the severity of AWD increased by allowing the soil to dry out more between flood events, yields declined while the other benefits increased. The reduction in GWP was mostly attributed to a reduction in CH4 emissions as changes in N2O emissions were minimal among treatments. When AWD was practiced early in the growing season followed by flooding for remainder of season, similar yields as the flooded control were obtained but reduced water use (18%), GWP (45%) and yield-scaled GWP (45%); although grain As concentrations were similar or higher. This highlights that multiple environmental benefits can be realized without sacrificing yield but there may be trade-offs to consider. Importantly, adoption of these practices will require that they are economically attractive and can be adapted to field scales. C1 [Linquist, Bruce A.; Adviento-Borbe, Maria Arlene A.; Van Kessel, Chris] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Anders, Merle M.] Univ Arkansas, Dept Crop Soil & Environm Sci, Rice Res & Extens Ctr, Stuttgart, AR 72160 USA. [Chaney, Rufus L.] USDA, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Nalley, L. Lanier] Univ Arkansas, Dept Agr Econ & Agribusiness, Fayetteville, AR 72701 USA. [Da Rosa, Eliete F. F.] Fed Inst Sci Educ & Technol Catarina, BR-89700 Florianopolis, SC, Brazil. RP Linquist, BA (reprint author), Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. EM balinquist@ucdavis.edu FU Mars, Inc.; RiceTec, Inc. FX We thank Mars, Inc. and RiceTec, Inc. for providing funding for this research and Shelly Kerr and David Hendrix for all the help in the field collecting samples. NR 65 TC 22 Z9 23 U1 14 U2 121 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JAN PY 2015 VL 21 IS 1 BP 407 EP 417 DI 10.1111/gcb.12701 PG 11 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX1HJ UT WOS:000346698100036 PM 25099317 ER PT J AU Kurnianto, S Warren, M Talbot, J Kauffman, B Murdiyarso, D Frolking, S AF Kurnianto, Sofyan Warren, Matthew Talbot, Julie Kauffman, Boone Murdiyarso, Daniel Frolking, Steve TI Carbon accumulation of tropical peatlands over millennia: a modeling approach SO GLOBAL CHANGE BIOLOGY LA English DT Article DE carbon sequestration; holocene; land-use change; oil palm; peat carbon stocks; peat swamp forests ID PEAT SWAMP FOREST; CLIMATE-CHANGE; EL-NINO; PENINSULAR MALAYSIA; LAST DEGLACIATION; NORTHERN PEATLAND; LATE PLEISTOCENE; HOLOCENE; INDONESIA; SEA AB Tropical peatlands cover an estimated 440000km(2) (similar to 10% of global peatland area) and are significant in the global carbon cycle by storing about 40-90 Gt C in peat. Over the past several decades, tropical peatlands have experienced high rates of deforestation and conversion, which is often associated with lowering the water table and peat burning, releasing large amounts of carbon stored in peat to the atmosphere. We present the first model of long-term carbon accumulation in tropical peatlands by modifying the Holocene Peat Model (HPM), which has been successfully applied to northern temperate peatlands. Tropical HPM (HPMTrop) is a one-dimensional, nonlinear, dynamic model with a monthly time step that simulates peat mass remaining in annual peat cohorts over millennia as a balance between monthly vegetation inputs (litter) and monthly decomposition. Key model parameters were based on published data on vegetation characteristics, including net primary production partitioned into leaves, wood, and roots; and initial litter decomposition rates. HPMTrop outputs are generally consistent with field observations from Indonesia. Simulated long-term carbon accumulation rates for 11000-year-old inland, and 5000-year-old coastal peatlands were about 0.3 and 0.59 Mg Cha(-1)yr(-1), and the resulting peat carbon stocks at the end of the 11000-year and 5000-year simulations were 3300 and 2900 Mg Cha(-1), respectively. The simulated carbon loss caused by coastal peat swamp forest conversion into oil palm plantation with periodic burning was 1400 Mg Cha(-1) over 100years, which is equivalent to similar to 2900years of C accumulation in a hectare of coastal peatlands. C1 [Kurnianto, Sofyan; Talbot, Julie; Frolking, Steve] Univ New Hampshire, Inst Study Earth Oceans & Space, Durham, NH 03824 USA. [Kurnianto, Sofyan; Talbot, Julie; Frolking, Steve] Univ New Hampshire, Dept Earth Sci, Durham, NH 03824 USA. [Kurnianto, Sofyan; Murdiyarso, Daniel] Ctr Int Forestry Res, Bogor 16115, Indonesia. [Warren, Matthew] US Forest Serv, USDA, No Res Stn, Durham, NH 03824 USA. [Talbot, Julie] Univ Montreal, Dept Geog, Montreal, PQ H3C 3J7, Canada. [Kauffman, Boone] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA. [Murdiyarso, Daniel] Bogor Agr Univ, Dept Geophys & Meteorol, Bogor 16115, Indonesia. RP Frolking, S (reprint author), Univ New Hampshire, Inst Study Earth Oceans & Space, Morse Hall 8,Coll Rd, Durham, NH 03824 USA. EM steve.frolking@unh.edu FU USDA Forest Service Indonesia Peatlands; Forest and Climate Change Initiative [USDA-2011-67003-30373]; University of New Hampshire; Center for International Forestry Research (CIFOR); USAID FX We thank R. Dommain for providing the peat core age-depth profile data, and for helpful comments on the model. We thank R. Birdsey, R. Kolka, S. Neuzil, and three anonymous reviewers for helpful comments on an earlier draft. This study was supported by a grant from the USDA Forest Service Indonesia Peatlands, Forest and Climate Change Initiative to SF (USDA-2011-67003-30373), a graduate tuition fellowship to SK from the University of New Hampshire, and the Center for International Forestry Research (CIFOR). Additional support from the USAID-funded Sustainable Wetlands Adaptation and Mitigation Program (SWAMP) was jointly implemented by CIFOR, the USFS, and Oregon State University. NR 73 TC 11 Z9 12 U1 5 U2 73 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1354-1013 EI 1365-2486 J9 GLOBAL CHANGE BIOL JI Glob. Change Biol. PD JAN PY 2015 VL 21 IS 1 BP 431 EP 444 DI 10.1111/gcb.12672 PG 14 WC Biodiversity Conservation; Ecology; Environmental Sciences SC Biodiversity & Conservation; Environmental Sciences & Ecology GA AX1HJ UT WOS:000346698100038 PM 25044171 ER PT J AU Olsen, SC Carlson, SA AF Olsen, Steven C. Carlson, Steve A. TI In vitro bactericidal activity of aminoglycosides, including the next-generation drug plazomicin, against Brucella spp. SO INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS LA English DT Article DE Brucella; Antibiotic; Aminoglycoside ID GENTAMICIN AB Plazomicin is a next-generation aminoglycoside with a potentially unique set of clinical characteristics compared with other aminoglycosides. This study assessed the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of plazomicin against 15 clinical isolates as well as three reference strains representing Brucella abortus, Brucella melitensis and Brucella suis. These data were compared with those obtained for six other aminoglycosides and two aminocyclitols. Plazomicin and gentamicin were the only drugs demonstrating bactericidal activity towards two of the three Brucella spp., whilst plazomicin was the only drug exhibiting bactericidal activity against B. suis. This is the first study to assess the bactericidal nature of plazomicin against Brucella spp. in vitro. (C) 2014 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved. C1 [Olsen, Steven C.] Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, Ames, IA 50010 USA. [Carlson, Steve A.] Iowa State Univ, Coll Vet Med, Dept Biomed Sci, Ames, IA 50011 USA. RP Carlson, SA (reprint author), Iowa State Univ, Coll Vet Med, Dept Biomed Sci, Ames, IA 50011 USA. EM stevec@istate.du FU US Department of Agriculture FX US Department of Agriculture. NR 10 TC 6 Z9 6 U1 1 U2 5 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0924-8579 EI 1872-7913 J9 INT J ANTIMICROB AG JI Int. J. Antimicrob. Agents PD JAN PY 2015 VL 45 IS 1 BP 76 EP 78 DI 10.1016/j.ijantimicag.2014.09.011 PG 3 WC Infectious Diseases; Microbiology; Pharmacology & Pharmacy SC Infectious Diseases; Microbiology; Pharmacology & Pharmacy GA AX7OU UT WOS:000347105800012 PM 25459738 ER PT J AU Walter, JA Meixler, MS Mueller, T Fagan, WF Tobin, PC Haynes, KJ AF Walter, Jonathan A. Meixler, Marcia S. Mueller, Thomas Fagan, William F. Tobin, Patrick C. Haynes, Kyle J. TI How topography induces reproductive asynchrony and alters gypsy moth invasion dynamics SO JOURNAL OF ANIMAL ECOLOGY LA English DT Article DE critical threshold; mating failure; phenology; positive density dependence; protandry ID SMALL-MAMMAL ABUNDANCE; LYMANTRIA-DISPAR L.; MATING SUCCESS; LEPIDOPTERA LYMANTRIIDAE; UNITED-STATES; GEOGRAPHICAL VARIATION; BIOLOGICAL INVASIONS; POPULATION-DYNAMICS; NORTH-AMERICA; DISPERSAL AB Reproductive asynchrony, a temporal mismatch in reproductive maturation between an individual and potential mates, may contribute to mate-finding failure and Allee effects that influence the establishment and spread of invasive species. Variation in elevation is likely to promote variability in maturation times for species with temperature-dependent development, but it is not known how strongly this influences reproductive asynchrony or the population growth of invasive species. We examined whether spatial variation in reproductive asynchrony, due to differences in elevation and local heterogeneity in elevation (hilliness), can explain spatial heterogeneity in the population growth rate of the gypsy moth, Lymantria dispar (L.), along its invasion front in Virginia and West Virginia, USA. We used a spatially explicit model of the effects of reproductive asynchrony on mating success to develop predictions of the influences of elevation and elevational heterogeneity on local population growth rates. Population growth rates declined with increased elevation and more modestly with increased elevational heterogeneity. As in earlier work, we found a positive relationship between the population growth rate and the number of introduced egg masses, indicating a demographic Allee effect. At high elevations and high heterogeneity in elevation, the population growth rate was lowest and the density at which the population tended to replace itself (i.e. the Allee threshold) was highest. An analysis of 22years of field data also showed decreases in population growth rates with elevation and heterogeneity in elevation that were largely consistent with the model predictions. These results highlight how topographic characteristics can affect reproductive asynchrony and influence mate-finding Allee effects in an invading non-native insect population. Given the dependence of developmental rates on temperature in poikilotherms, topographic effects on reproductive success could potentially be important to the population dynamics of many organisms. C1 [Walter, Jonathan A.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22904 USA. [Walter, Jonathan A.; Haynes, Kyle J.] Univ Virginia, Boyce, VA 22620 USA. [Meixler, Marcia S.] Rutgers State Univ, Dept Ecol Evolut & Nat Resources, New Brunswick, NJ 08901 USA. [Meixler, Marcia S.; Mueller, Thomas; Fagan, William F.] Univ Maryland, Dept Biol, College Pk, MD 20742 USA. [Tobin, Patrick C.] US Forest Serv, USDA, No Res Stn, Morgantown, WV 26505 USA. RP Walter, JA (reprint author), Univ Virginia, Dept Environm Sci, Charlottesville, VA 22904 USA. EM jwalter@virginia.edu RI Haynes, Kyle/C-1374-2012 OI Haynes, Kyle/0000-0002-3283-6633 FU NSF [DMS 1225917]; University of Maryland; University of Virginia's Blandy Experimental Farm FX The authors thank Janis Antonovics for advice on GAMs. Janis Antonovis, Andrew Allstadt, the University of Virginia terrestrial ecology group and two anonymous reviewers provided helpful comments on the manuscript. Remi St-Amant and Christelle Robinet provided gypsy moth phenological predictions. Laura Blackburn and Matt O'Connell provided technical assistance. This work was supported in part by NSF grant DMS 1225917 to WFF, the University of Maryland, and the University of Virginia's Blandy Experimental Farm. We also acknowledge the gypsy moth Slow-the-Spread Foundation, Inc., for access to trap catch data. NR 71 TC 2 Z9 2 U1 8 U2 44 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0021-8790 EI 1365-2656 J9 J ANIM ECOL JI J. Anim. Ecol. PD JAN PY 2015 VL 84 IS 1 BP 188 EP 198 DI 10.1111/1365-2656.12272 PG 11 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AX4NO UT WOS:000346909300018 PM 25039257 ER PT J AU Annweiler, C Dursun, E Feron, F Gezen-Ak, D Kalueff, AV Littlejohns, T Llewellyn, DJ Millet, P Scott, T Tucker, KL Yilmazer, S Beauchet, O AF Annweiler, C. Dursun, E. Feron, F. Gezen-Ak, D. Kalueff, A. V. Littlejohns, T. Llewellyn, D. J. Millet, P. Scott, T. Tucker, K. L. Yilmazer, S. Beauchet, O. TI 'Vitamin D and cognition in older adults': updated international recommendations SO JOURNAL OF INTERNAL MEDICINE LA English DT Review DE Alzheimer's disease; brain; cognition; neuroendocrinology; older adults; vitamin D ID D-RECEPTOR GENE; RANDOMIZED CONTROLLED-TRIAL; VDR PATHWAY DISRUPTION; ALZHEIMERS-DISEASE; HIPPOCAMPAL-NEURONS; 25-HYDROXYVITAMIN D; AMYLOID-BETA; D DEFICIENCY; CEREBROSPINAL-FLUID; D SUPPLEMENTATION AB BackgroundHypovitaminosis D, a condition that is highly prevalent in older adults aged 65years and above, is associated with brain changes and dementia. Given the rapidly accumulating and complex contribution of the literature in the field of vitamin D and cognition, clear guidance is needed for researchers and clinicians. MethodsInternational experts met at an invitational summit on Vitamin D and Cognition in Older Adults'. Based on previous reports and expert opinion, the task force focused on key questions relating to the role of vitamin D in Alzheimer's disease and related disorders. Each question was discussed and voted using a Delphi-like approach. ResultsThe experts reached an agreement that hypovitaminosis D increases the risk of cognitive decline and dementia in older adults and may alter the clinical presentation as a consequence of related comorbidities; however, at present, vitamin D level should not be used as a diagnostic or prognostic biomarker of Alzheimer's disease due to lack of specificity and insufficient evidence. This population should be screened for hypovitaminosis D because of its high prevalence and should receive supplementation, if necessary; but this advice was not specific to cognition. During the debate, the possibility of critical periods' during which vitamin D may have its greatest impact on the brain was addressed; whether hypovitaminosis D influences cognition actively through deleterious effects and/or passively by loss of neuroprotection was also considered. ConclusionsThe international task force agreed on five overarching principles related to vitamin D and cognition in older adults. Several areas of uncertainty remain, and it will be necessary to revise the proposed recommendations as new findings become available. C1 [Annweiler, C.; Beauchet, O.] Angers Univ Hosp, Div Geriatr Med, Dept Neurosci, F-49933 Angers, France. [Annweiler, C.; Beauchet, O.] Angers Univ Hosp, UNAM, Memory Clin, UPRES EA 4638, F-49933 Angers, France. [Annweiler, C.] Univ Western Ontario, Schulich Sch Med & Dent, Robarts Res Inst, Dept Med Biophys, London, ON, Canada. [Dursun, E.; Gezen-Ak, D.; Yilmazer, S.] Istanbul Univ, Cerrahpasa Fac Med, Dept Med Biol, Istanbul, Turkey. [Feron, F.; Millet, P.] Aix Marseille Univ, CNRS, NICN UMR 7259, Marseille, France. [Kalueff, A. V.] ZENEREI Inst, Slidell, LA USA. [Llewellyn, D. J.; Millet, P.] Univ Exeter, Sch Med, Exeter, Devon, England. [Scott, T.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Tucker, K. L.] Univ Massachusetts, Dept Clin Lab & Nutr Sci, Lowell, MA USA. RP Annweiler, C (reprint author), Angers Univ Hosp, Div Geriatr Med, Dept Neurosci, F-49933 Angers, France. EM CeAnnweiler@chu-angers.fr OI Annweiler, Cedric/0000-0002-7199-8109 FU Center for Research on Autonomy and Longevity (CeRAL), University Hospital of Angers, France; Istanbul University [460, 548, 4426, ONAP-21712, ONAP-28651]; Scientific and Technological Research Council of Turkey-TUBITAK [107S041, 111S200]; Health, Sport and Sustainable Development Foundation of Aix-Marseille University; Alzheimer's Association [NIRG-11-200737]; Mary Kinross Charitable Trust; James Tudor Foundation; Halpin Trust; Sir Halley Stewart Trust; Age Related Diseases and Health Trust; Norman Family Charitable Trust; UK National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care (CLAHRC) for the South West Peninsula FX The summit and this work were supported by the Center for Research on Autonomy and Longevity (CeRAL), University Hospital of Angers, France. ED, DGA and SY are supported by the Research Fund of Istanbul University (Project No: 460, 548, 4426, ONAP-21712 and ONAP-28651) and by the Scientific and Technological Research Council of Turkey-TUBITAK (Project No. 107S041 and 111S200). FF and PM are supported by the Health, Sport and Sustainable Development Foundation of Aix-Marseille University. DJL and TL: Additional support was provided from the Alzheimer's Association by NIRG-11-200737, the Mary Kinross Charitable Trust, the James Tudor Foundation, the Halpin Trust, the Sir Halley Stewart Trust, the Age Related Diseases and Health Trust, and the Norman Family Charitable Trust. DJL and TL are also supported by the UK National Institute for Health Research (NIHR) Collaboration for Leadership in Applied Health Research and Care (CLAHRC) for the South West Peninsula. The sponsors had no role in the design and conduct of the study, in the collection, management, analysis and interpretation of the data, or in the preparation, review or approval of the manuscript. The views expressed in this publication are those of the authors and not necessarily those of the funding sources. NR 76 TC 23 Z9 24 U1 1 U2 18 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0954-6820 EI 1365-2796 J9 J INTERN MED JI J. Intern. Med. PD JAN PY 2015 VL 277 IS 1 BP 45 EP 57 DI 10.1111/joim.12279 PG 13 WC Medicine, General & Internal SC General & Internal Medicine GA AX0OQ UT WOS:000346652000003 PM 24995480 ER PT J AU Marini, JC AF Marini, Juan C. TI Protein Requirements: Are We Ready for New Recommendations? SO JOURNAL OF NUTRITION LA English DT Editorial Material ID ACID OXIDATION TECHNIQUE; DIETARY-PROTEIN; WOMEN; AGE C1 [Marini, Juan C.] Baylor Coll Med, Dept Pediat, Sect Crit Care Med, Houston, TX 77030 USA. [Marini, Juan C.] Baylor Coll Med, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. RP Marini, JC (reprint author), Baylor Coll Med, Dept Pediat, Sect Crit Care Med, Houston, TX 77030 USA. EM marini@bcm.edu NR 19 TC 1 Z9 1 U1 0 U2 1 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JAN PY 2015 VL 145 IS 1 BP 5 EP 6 DI 10.3945/jn.114.203935 PG 2 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AY0BS UT WOS:000347263600002 PM 25342701 ER PT J AU Bird, JK Ronnenberg, AG Choi, SW Du, FL Mason, JB Liu, ZH AF Bird, Julia K. Ronnenberg, Alayne G. Choi, Sang-Woon Du, Fangling Mason, Joel B. Liu, Zhenhua TI Obesity Is Associated with Increased Red Blood Cell Folate Despite Lower Dietary Intakes and Serum Concentrations SO JOURNAL OF NUTRITION LA English DT Article DE folate metabolism; NHANES; obesity; adults; B-vitamins; biomarkers; blood ID BODY-MASS INDEX; BREAST-CANCER; CHILDBEARING AGE; FOLIC-ACID; POSTMENOPAUSAL WOMEN; PLASMA FOLATE; UNITED-STATES; RISK; METABOLISM; METAANALYSIS AB Background: Folates are essential cofactors in metabolic pathways that facilitate biological methylation and nucleotide synthesis, and therefore have widespread effects on health and diseases. Although obesity is prevalent worldwide, few studies have investigated how obesity interacts with folate status. Objective: Based on data from the NHANES, this study aims to examine the association between body mass index (BM I) and obesity-related metabolic factors with blood folate status. Methods: A nationally representative sample of 3767 adults from the NHANES (2003-2006) was used as the study population. Regression analyses, with and without adjustment for demographic factors and dietary intakes, were performed to examine associations between BMI and metabolic factors with serum and RBC folate. Results: The results indicate serum folate concentrations were lower in obese groups compared to the desirable BM I and overweight categories, paralleling lower intakes in this group. In contrast, RBC folate increased incrementally with BMI. Regression analyses demonstrated an inverse relation between BM I and serum folate but a positive relation for RBC folate (P < 0.01). Waist circumference, serum triglycerides, and fasting plasma glucose each displayed significant positive relations with RBC folate (P < 0.01), although relations with serum folate were not significant and consistent. Conclusions: In summary, obesity is associated with decreased serum folate, which parallels decreased folate intakes. In contrast, obesity is positively associated with RBC folate. Therefore, RBC folate, in addition to serum folate, should also be considered as a critical biomarker for folate status, especially in the obese population. Future research is needed to understand how obesity differentially alters serum and RBC folate status because they are associated with a variety of medical complications. C1 [Bird, Julia K.; Choi, Sang-Woon; Liu, Zhenhua] Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Amherst, MA 01003 USA. [Ronnenberg, Alayne G.; Choi, Sang-Woon] CHA Univ, Sch Med, Chaum Life Ctr, Seoul, South Korea. [Du, Fangling] Shandong Acad Agr Sci, Inst Food Sci & Technol, Jinan, Peoples R China. [Mason, Joel B.] Tufts Med Ctr, Ctr Canc, Boston, MA USA. [Mason, Joel B.; Liu, Zhenhua] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. RP Liu, ZH (reprint author), Univ Massachusetts, Sch Publ Hlth & Hlth Sci, Amherst, MA 01003 USA. EM zliu@nutrition.umass.edu OI Bird, Julia/0000-0001-6015-3576 FU USDA [2014-67017-21762]; USDA Hatch grant [MAS00454]; Rays of Hope Center of Breast Cancer Research; Baystate Medical Center; USDA Agricultural Research Service [1950-5100-074-01S]; [R21 ES019102] FX Supported in part by a USDA grant (2014-67017-21762 to ZL), a USDA Hatch grant (MAS00454 to ZL), funding from Rays of Hope Center of Breast Cancer Research, Baystate Medical Center (to ZL), R21 ES019102 (to JBM), and the USDA Agricultural Research Service (Agreement No. 1950-5100-074-01S). NR 51 TC 12 Z9 12 U1 2 U2 13 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JAN PY 2015 VL 145 IS 1 BP 79 EP 86 DI 10.3945/jn.114.199117 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AY0BS UT WOS:000347263600012 PM 25527662 ER PT J AU Nicklas, TA O'Neil, CE Fulgoni, VL AF Nicklas, Theresa A. O'Neil, Carol E. Fulgoni, Victor L., III TI Differing Statistical Approaches Affect the Relation between Egg Consumption, Adiposity, and Cardiovascular Risk Factors in Adults SO JOURNAL OF NUTRITION LA English DT Article DE adults; cardiovascular risk factors; egg consumption; statistical approaches; food patterns ID MULTIPLE-PASS METHOD; HEART-DISEASE RISK; DIETARY PATTERNS; EPIDEMIOLOGY; METAANALYSIS; US; CHOLESTEROL; ALCOHOL; BEER; WINE AB Background: Associations between food patterns and adiposity are poorly understood. Objective: Two statistical approaches were used to examine the potential association between egg consumption and adiposity. Methods: Participants (n = 18,987) aged >= 19 y were from the 2001-2008 NHANES who provided 24-h diet recall data, body mass index (BMI) and waist circumference (WC) determined adiposity measures, and blood pressure, circulating insulin, glucose, and lipid concentrations were considered cardiovascular risk factors (CVRFs). Covariate-adjusted least-squares means +/- SEs were generated. Results: The first statistical approach categorized participants into egg consumers or nonconsumers. Consumers had higher mean BMI (in kg/m(2); 28.7 +/- 0.19; P = 0.006) and WC (98 2 +/- 0.43 cm; P= 0.002) than did nonconsumers (28.2 +/- 0.10 and 96.9 +/- 0.23 cm, respectively). Second, cluster analysis identified 8 distinct egg consumption patterns (explaining 39.5% of the variance in percentage of energy within the food categories). Only 2 egg patterns [egg/meat, poultry, fish (MPF)/grains/vegetables and egg/MPF/grains], consumed by <= 2% of the population, drove the association (compared with the no-egg pattern) between egg consumption and BMI and WC. Another analysis controlled for the standard covariates and the other food groups consumed with eggs in those 2 egg patterns. Only the egg/MPF/other-grains pattern remained associated with BM( and WC (both P <= 0.0063). The pattern analyses identified associations between an egg pattern (egg/MPF/other grains/potatoes/other beverages) and diastolic blood pressure (DBP) and serum LDL cholesterol (both <= 0.0063). A final analysis was conducted by adding percentage of energy from fast foods and medication use for diabetes to the covariates. The association between the egg/MPF/grains pattern and BMI and the egg/MPF/potatoes/other beverages and DBP and LDL cholesterol disappeared. Conclusions: Care needs to be taken with data interpretation of diet and health risk factors and the choice of statistical analyses and covariates used in the analyses because these studies are typically used to generate hypotheses. Additional studies are needed to better understand these relations. C1 [Nicklas, Theresa A.] Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [O'Neil, Carol E.] Louisiana State Univ, Ctr Agr, Sch Nutr & Food Sci, Baton Rouge, LA 70803 USA. [Fulgoni, Victor L., III] Nutr Impact LLC, Battle Creek, MI USA. RP Nicklas, TA (reprint author), Baylor Coll Med, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM tnicklas@bcm.edu FU Egg Nutrition Center; American Egg Board; USDA/Agricultural Research Service [58-6250-0-008]; Egg Nutrition Board; USDA Hatch Project [LAB 93951] FX Presented at the conference "Advances and Controversies in Clinical Nutrition," held in Washington, DC, 5-7 December 2013. The conference was sponsored by the Egg Nutrition Center and the American Egg Board. The Supplement Coordinator for this supplement was Tia M Rains. Supplement Coordinator disclosures: Tia M Rains is an employee of The Egg Nutrition Center/American Egg Board The Session Chair was Mitch Kanter. This supplement is the responsibility of the Guest Editor to whom the Editor of The Journal of Nutrition has delegated supervision of both technical conformity to the published regulations of The Journal of Nutrition and general oversight of the scientific merit of each article. The Guest Editor for this supplement was Harry D Dawson. Guest Editor disclosure: no conflicts of interest Publication costs for this supplement were defrayed in part by the payment of page charges. This publication must therefore be hereby marked "advertisement" in accordance with 18 USC section 1734 solely to indicate this fact. The opinions expressed in this publication are those of the authors and are not attributable to the sponsors or the publisher, Editor, or Editorial Board of The Journal of Nutrition.; Supported by the USDA/Agricultural Research Service through specific cooperative agreement 58-6250-0-008 Partial support was received from the Egg Nutrition Board and from the USDA Hatch Project LAB 93951. NR 46 TC 4 Z9 4 U1 0 U2 14 PU AMER SOC NUTRITION-ASN PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0022-3166 EI 1541-6100 J9 J NUTR JI J. Nutr. PD JAN PY 2015 VL 145 IS 1 BP 170 EP 176 DI 10.3945/jn.114.194068 PG 7 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AY0BS UT WOS:000347263600025 PM 25527676 ER PT J AU Lehman, RM Acosta-Martinez, V Buyer, JS Cambardella, CA Collins, HP Ducey, TF Halvorson, JJ Jin, VL Johnson, JMF Kremer, RJ Lundgren, JG Manter, DK Maul, JE Smith, JL Stott, DE AF Lehman, R. Michael Acosta-Martinez, Veronica Buyer, Jeffrey S. Cambardella, Cynthia A. Collins, Harold P. Ducey, Thomas F. Halvorson, Jonathan J. Jin, Virginia L. Johnson, Jane M. F. Kremer, Robert J. Lundgren, Jonathan G. Manter, Daniel K. Maul, Jude E. Smith, Jeffrey L. Stott, Diane E. TI Soil biology for resilient, healthy soil SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article ID AMMONIA-OXIDIZING PROKARYOTES; MICROBIAL COMMUNITIES; BACTERIAL DIVERSITY; BIODIVERSITY; RHIZOSPHERE; MANAGEMENT; QUALITY; RESISTANCE; FRAMEWORK; ABUNDANCE C1 [Lehman, R. Michael; Lundgren, Jonathan G.] USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. [Acosta-Martinez, Veronica] USDA ARS, Cropping Syst Res Lab, Lubbock, TX 79401 USA. [Buyer, Jeffrey S.; Maul, Jude E.] USDA ARS, Sustainable Agr Syst Lab, Beltsville, MD USA. [Cambardella, Cynthia A.] USDA ARS, Natl Lab Agr & Environm, Ames, IA USA. [Collins, Harold P.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Ducey, Thomas F.] USDA ARS, Coastal Plain Soil Water & Plant Conservat Res La, Florence, SC USA. [Halvorson, Jonathan J.] USDA ARS, No Great Plains Res Lab, Mandan, ND 58554 USA. [Jin, Virginia L.] USDA ARS, Agroecosyst Management Res Unit, Lincoln, NE USA. [Johnson, Jane M. F.] USDA ARS, N Cent Soil Conservat Res Lab, Morris, MN 56267 USA. [Kremer, Robert J.] USDA ARS, Cropping Syst & Water Qual Res Lab, Columbia, MO USA. [Manter, Daniel K.] USDA ARS, Soil Plant & Nutr Res Lab, Ft Collins, CO 80522 USA. [Smith, Jeffrey L.] USDA ARS, Land Management & Water Conservat Res Lab, Pullman, WA USA. [Stott, Diane E.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN USA. RP Lehman, RM (reprint author), USDA ARS, North Cent Agr Res Lab, Brookings, SD 57006 USA. OI Ducey, Thomas/0000-0001-8199-0584; Lehman, Michael/0000-0002-3391-3178; Johnson, Jane/0000-0002-1687-4007 NR 41 TC 10 Z9 11 U1 2 U2 34 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JAN-FEB PY 2015 VL 70 IS 1 BP 12A EP 18A DI 10.2489/jswc.70.1.12A PG 7 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AX8UI UT WOS:000347183400002 ER PT J AU Ogg, C AF Ogg, Clayton TI Compliance programs' feasibility for reducing worldwide loss of forests and other ecosystems SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Editorial Material C1 [Ogg, Clayton] USDA, Washington, DC USA. [Ogg, Clayton] US EPA, Washington, DC USA. NR 22 TC 0 Z9 0 U1 0 U2 0 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JAN-FEB PY 2015 VL 70 IS 1 BP 19A EP 22A DI 10.2489/jswc.70.1.19A PG 4 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AX8UI UT WOS:000347183400003 ER PT J AU Francesconi, W Smith, DR Heathman, GC Gonzalez, JM Benage, MM AF Francesconi, W. Smith, D. R. Heathman, G. C. Gonzalez, J. M. Benage, M. M. TI Application of a calibrated/validated Agricultural Policy/Environmental eXtender model to assess sediment and nutrient delivery from the Wildcat Creek Mississippi River Basin Initiative-Cooperative Conservation Partnership Initiative SO JOURNAL OF SOIL AND WATER CONSERVATION LA English DT Article DE agriculture conservation practices; Agriculture Policy/Environmental eXtender; Conservation Effects Assessment Project; modeling; watershed ID WATER ASSESSMENT-TOOL; WINTER COVER CROPS; APEX MODEL; NO-TILL; SOIL; PHOSPHORUS; QUALITY; CORN; CALIBRATION; VALIDATION AB Wildcat Creek, a tributary to the Wabash River, was identified by the USDA Natural Resources Conservation Service (NRCS) as a priority watershed for its high sediment and nutrient loading contributions to the Mississippi River. As part of the Mississippi River Basin Initiative (MRBI), the incorporation of conservation practices was implemented through a Cooperative Conservation Partnership Initiative (CCPI) agreement using existing USDA programs. The objective of this work was to evaluate the potential water quality benefits of conservation practices placed in the Wildcat Creek Watershed through the MRBI-CCPI. Specifically, seven output variables (surface runoff, tile flow, sediments, total phosphorus [P], soluble P, soluble nitrogen [N], and soluble N in tile) were compared for four different row crop conservation practices (residue and tillage management no-till, residue and tillage management mulch-till, cover crops, and forage and biomass planting), and two manure application scenarios. Using the Agricultural Policy/Environmental eXtender (APEX) model, calibrated and validated for a field within the St. Joseph River Watershed, a modeling assessment was developed for current conservation practices incorporated at a similar watershed, Wildcat Creek. Annual average values were estimated for each simulated scenario, and percentage reductions were reported. Cover crops consistently reduced sediment and nutrient loads in runoff by more than 50% compared to the tillage baseline scenario, and removal of the manure application in the waste utilization scenario reduced the predicted total and soluble P amounts by 75% and 92%, respectively. When extrapolating the edge-of-field values predicted by the model to the area occupied by each conservation practice throughout the duration of the contracts at the Wildcat Creek Watershed, the total reductions were estimated to be almost 7,000 Mg (7,700 tn) of sediment (68% decrease), 20,000 kg (44,092 lb) of total P (73% decrease), 4,000 kg (8,818.5 lb) of soluble N in runoff (50% decrease), and 185,000 kg (414,00 lb) of soluble N in tile flow (43% decrease). Agricultural Policy/Environmental eXtender modeling is a useful tool for predicting sediment and nutrient loading information at the field-scale, which can be used to calculate the relative impact of conservation practices at the watershed scale when monitoring data are not available. The modeling results confirm the positive impact of these conservation practices and of the MRBI-CCPI initiative at improving water quality at the edge-of-field, and potentially at the Wildcat Creek, the Greater Wabash River Basins, and ultimately the Mississippi River Basin. C1 [Francesconi, W.; Smith, D. R.; Heathman, G. C.; Gonzalez, J. M.; Benage, M. M.] USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. RP Francesconi, W (reprint author), USDA ARS, Natl Soil Eros Res Lab, W Lafayette, IN 47907 USA. NR 57 TC 1 Z9 1 U1 5 U2 26 PU SOIL WATER CONSERVATION SOC PI ANKENY PA 945 SW ANKENY RD, ANKENY, IA 50023-9723 USA SN 0022-4561 EI 1941-3300 J9 J SOIL WATER CONSERV JI J. Soil Water Conserv. PD JAN-FEB PY 2015 VL 70 IS 1 BP 23 EP 35 DI 10.2489/jswc.70.1.23 PG 13 WC Ecology; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA AX8UI UT WOS:000347183400006 ER PT J AU Taylor, MM Bumanlag, LP Lee, J Latona, NP Brown, EM Liu, CK AF Taylor, M. M. Bumanlag, L. P. Lee, J. Latona, N. P. Brown, E. M. Liu, C. -K. TI UTILIZATION OF AGRICULTURAL BY-PRODUCTS TO PARTIALLY REPLACE GELATIN IN PREPARATION OF PRODUCTS FOR LEATHER SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID OXIDIZED PHENOLIC-COMPOUNDS; MECHANICAL-PROPERTIES; MODIFIED PROTEINS; FERULIC ACID; TANNIC-ACID; SURIMI; FILMS; GELS AB When polyphenolic-modified gelatin-products were used as fillers, improvements were seen in the subjective properties of the leather. When the treated samples were compared to control samples, there were no significant changes in mechanical properties. At the present time, gelatin is in short supply, costs are increasing, and there is an urgent need to find a substitute that could be combined with the gelatin, thereby, partially replacing and reducing the amount of gelatin required, with the goal that the new products would retain the desired characteristics of gelatin products. We have evaluated the potential of producing biopolymers from the reaction of polyphenols with gelatin in combination with other proteins (e.g. whey) or with carbohydrates (e.g. chitosan and pectin). Several researchers have recently demonstrated the feasibility of these reactions. These combinations would take advantage of the distinctive properties of both species and at the same time create products with improved functional properties. Recently, the preparation of polyphenolic-modified gelatin/whey biopolymer products was investigated, and the results of product characterization using physicochemical analyses indicated optimal products that could be used as fillers. In this continuing study, these products were applied to wet white, that was then finished, and subjective and mechanical properties were evaluated. At the same time a method was developed to determine the rate of uptake of the product. Results of the studies will be presented. These findings could further add to the knowledge of using renewable resources in production of unique products that may have leather processing application. C1 [Taylor, M. M.; Bumanlag, L. P.; Lee, J.; Latona, N. P.; Brown, E. M.; Liu, C. -K.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Taylor, MM (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM maryann.taylor@ars.usda.gov NR 21 TC 1 Z9 1 U1 2 U2 5 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD JAN PY 2015 VL 110 IS 1 BP 13 EP 18 PG 6 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA AY1QE UT WOS:000347366400003 ER PT J AU Brown, EM Taylor, MM Bumanlag, L AF Brown, Eleanor M. Taylor, Maryann M. Bumanlag, Lorelie TI POWDERED HIDE MODEL FOR VEGETABLE TANNING II: HYDROLYZABLE TANNIN SO JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION LA English DT Article ID COLLAGEN MICROFIBRIL; TANNAGE AB Vegetable tannages employ both condensed and hydrolyzable tannins. As part of our exploration of tanning mechanisms, we reported previously on interactions of the condensed tannin, quebracho, with powdered hide. In this study, the interactions of chestnut extract, a hydrolyzable tannin, with powdered hide samples are reported and compared with those of the condensed tannin. Hydrothermal stability of powdered hide treated with the hydrolyzable tannin reached a maximum of 75 C at a 40% offer, compared with 84 C for a similar offer of condensed tannin. The hydrolyzable tannin was much more effective at improving collagenase resistance, with nearly complete protection at <10% offer. C1 [Brown, Eleanor M.; Taylor, Maryann M.; Bumanlag, Lorelie] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Brown, EM (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM eleanor.brown@ars.usda.gov NR 17 TC 1 Z9 1 U1 1 U2 4 PU AMER LEATHER CHEMISTS ASSOC PI LUBBOCK PA 1314 50 ST, STE 103, LUBBOCK, TX 79412 USA SN 0002-9726 J9 J AM LEATHER CHEM AS JI J. Am. Leather Chem. Assoc. PD JAN PY 2015 VL 110 IS 1 BP 19 EP 22 PG 4 WC Chemistry, Applied; Materials Science, Textiles SC Chemistry; Materials Science GA AY1QE UT WOS:000347366400004 ER PT J AU Allison, AB Ballard, JR Tesh, RB Brown, JD Ruder, MG Keel, MK Munk, BA Mickley, RM Gibbs, SEJ da Rosa, APAT Ellis, JC Ip, HS Shearn-Bochsler, VI Rogers, MB Ghedin, E Holmes, EC Parrish, CR Dwyer, C AF Allison, Andrew B. Ballard, Jennifer R. Tesh, Robert B. Brown, Justin D. Ruder, Mark G. Keel, M. Kevin Munk, Brandon A. Mickley, Randall M. Gibbs, Samantha E. J. da Rosa, Amelia P. A. Travassos Ellis, Julie C. Ip, Hon S. Shearn-Bochsler, Valerie I. Rogers, Matthew B. Ghedin, Elodie Holmes, Edward C. Parrish, Colin R. Dwyer, Chris TI Cyclic Avian Mass Mortality in the Northeastern United States Is Associated with a Novel Orthomyxovirus SO JOURNAL OF VIROLOGY LA English DT Article ID ARGAS-PERSICARGAS-ARBOREUS; ENVELOPE FUSION PROTEIN; TICK-BORNE VIRUSES; ARANSAS-BAY-VIRUS; INFLUENZA-A; DHORI VIRUS; FAMILY ORTHOMYXOVIRIDAE; HYALOMMA-DROMEDARII; MACQUARIE ISLAND; MEMBRANE-FUSION AB Since 1998, cyclic mortality events in common eiders (Somateria mollissima), numbering in the hundreds to thousands of dead birds, have been documented along the coast of Cape Cod, MA, USA. Although longitudinal disease investigations have uncovered potential contributing factors responsible for these outbreaks, detecting a primary etiological agent has proven enigmatic. Here, we identify a novel orthomyxovirus, tentatively named Wellfleet Bay virus (WFBV), as a potential causative agent of these outbreaks. Genomic analysis of WFBV revealed that it is most closely related to members of the Quaranjavirus genus within the family Orthomyxoviridae. Similar to other members of the genus, WFBV contains an alphabaculovirus gp64-like glycoprotein that was demonstrated to have fusion activity; this also tentatively suggests that ticks (and/or insects) may vector the virus in nature. However, in addition to the six RNA segments encoding the prototypical structural proteins identified in other quaranjaviruses, a previously unknown RNA segment (segment 7) encoding a novel protein designated VP7 was discovered in WFBV. Although WFBV shows low to moderate levels of sequence similarity to Quaranfil virus and Johnston Atoll virus, the original members of the Quaranjavirus genus, additional antigenic and genetic analyses demonstrated that it is closely related to the recently identified Cygnet River virus (CyRV) from South Australia, suggesting that WFBV and CyRV may be geographic variants of the same virus. Although the identification of WFBV in part may resolve the enigma of these mass mortality events, the details of the ecology and epidemiology of the virus remain to be determined. IMPORTANCE The emergence or reemergence of viral pathogens resulting in large-scale outbreaks of disease in humans and/or animals is one of the most important challenges facing biomedicine. For example, understanding how orthomyxoviruses such as novel influenza A virus reassortants and/or mutants emerge to cause epidemic or pandemic disease is at the forefront of current global health concerns. Here, we describe the emergence of a novel orthomyxovirus, Wellfleet Bay virus (WFBV), which has been associated with cyclic large-scale bird die-offs in the northeastern United States. This initial characterization study provides a foundation for further research into the evolution, epidemiology, and ecology of newly emerging orthomyxoviruses, such as WFBV, and their potential impacts on animal and/or human health. C1 [Allison, Andrew B.; Parrish, Colin R.] Cornell Univ, Coll Vet Med, James A Baker Inst Anim Hlth, Dept Microbiol & Immunol, Ithaca, NY 14853 USA. [Allison, Andrew B.; Ballard, Jennifer R.; Brown, Justin D.; Ruder, Mark G.; Keel, M. Kevin; Munk, Brandon A.] Univ Georgia, Coll Vet Med, southeastern Cooperat Wildlife Dis Study, Athens, GA USA. [Tesh, Robert B.; da Rosa, Amelia P. A. Travassos] Univ Texas Med Branch, Dept Pathol, Ctr Biodef & Emerging Infect Dis, Galveston, TX 77555 USA. [Mickley, Randall M.] USDA, Anim & Plant Hlth Inspect Serv, Wildlife Serv, MA CT RI Program, Sutton, MA USA. [Gibbs, Samantha E. J.] US Fish & Wildlife Serv, US Dept Interior, Patuxent Res Refuge, Laurel, MD USA. [Ellis, Julie C.] Tufts Univ, Dept Infect Dis & Global Hlth, Cummings Sch Vet Med, North Grafton, MA USA. [Ip, Hon S.; Shearn-Bochsler, Valerie I.] US Geol Survey, Natl Wildlife Hlth Ctr, Madison, WI USA. [Rogers, Matthew B.; Ghedin, Elodie] Univ Pittsburgh, Ctr Vaccine Res, Dept Computat & Syst Biol, Pittsburgh, PA USA. [Holmes, Edward C.] Univ Sydney, Marie Beshir Inst Infect Dis & Biosecur, Charles Perkins Ctr, Sch Biol Sci, Sydney, NSW 2006, Australia. [Holmes, Edward C.] Univ Sydney, Sydney Med Sch, Sydney, NSW 2006, Australia. [Dwyer, Chris] US Fish & Wildlife Serv, US Dept Interior, Div Migratory Birds, Hadley, MA USA. RP Allison, AB (reprint author), Cornell Univ, Coll Vet Med, James A Baker Inst Anim Hlth, Dept Microbiol & Immunol, Ithaca, NY 14853 USA. EM aba75@cornell.edu OI Allison, Andrew B./0000-0003-0971-1215; Holmes, Edward/0000-0001-9596-3552 FU United States Department of the Interior (USDI) [F12AP0122]; wildlife management agencies of the SCWDS member states through the Federal Aid to Wildlife Restoration Act [50 Stat.917]; USDI [G11AC20003]; National Health and Medical Research Council Australia Fellowship; NRSA from the National Institute of Allergy and Infectious Diseases, National Institutes of Health [F32AI100545] FX Funding for this research was provided by United States Department of the Interior (USDI) Cooperative Agreement F12AP0122 to A.B.A. Additional support was provided by the wildlife management agencies of the SCWDS member states through the Federal Aid to Wildlife Restoration Act (50 Stat.917) and by USDI Cooperative Agreement G11AC20003. E.C.H. was supported by a National Health and Medical Research Council Australia Fellowship. A.B.A. is additionally supported through an NRSA postdoctoral fellowship (F32AI100545) from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. NR 84 TC 4 Z9 4 U1 2 U2 14 PU AMER SOC MICROBIOLOGY PI WASHINGTON PA 1752 N ST NW, WASHINGTON, DC 20036-2904 USA SN 0022-538X EI 1098-5514 J9 J VIROL JI J. Virol. PD JAN PY 2015 VL 89 IS 2 BP 1389 EP 1403 DI 10.1128/JVI.02019-14 PG 15 WC Virology SC Virology GA AX8SN UT WOS:000347178900043 PM 25392223 ER PT J AU Jane, SF Wilcox, TM McKelvey, KS Young, MK Schwartz, MK Lowe, WH Letcher, BH Whiteley, AR AF Jane, Stephen F. Wilcox, Taylor M. McKelvey, Kevin S. Young, Michael K. Schwartz, Michael K. Lowe, Winsor H. Letcher, Benjamin H. Whiteley, Andrew R. TI Distance, flow and PCR inhibition: eDNA dynamics in two headwater streams SO MOLECULAR ECOLOGY RESOURCES LA English DT Article DE eDNA; environmental DNA; fish; lotic; qPCR; stream ID REAL-TIME PCR; ANTIBIOTIC-RESISTANCE GENES; ENVIRONMENTAL DNA; DIAGNOSTIC PCR; WATER SAMPLES; BROOK TROUT; ORGANIC-MATTER; IDAHO STREAMS; TRANSPORT; AMPLIFICATION AB Environmental DNA (eDNA) detection has emerged as a powerful tool for monitoring aquatic organisms, but much remains unknown about the dynamics of aquatic eDNA over a range of environmental conditions. DNA concentrations in streams and rivers will depend not only on the equilibrium between DNA entering the water and DNA leaving the system through degradation, but also on downstream transport. To improve understanding of the dynamics of eDNA concentration in lotic systems, we introduced caged trout into two fishless headwater streams and took eDNA samples at evenly spaced downstream intervals. This was repeated 18 times from mid-summer through autumn, over flows ranging from approximately 1-96L/s. We used quantitative PCR to relate DNA copy number to distance from source. We found that regardless of flow, there were detectable levels of DNA at 239.5m. The main effect of flow on eDNA counts was in opposite directions in the two streams. At the lowest flows, eDNA counts were highest close to the source and quickly trailed off over distance. At the highest flows, DNA counts were relatively low both near and far from the source. Biomass was positively related to eDNA copy number in both streams. A combination of cell settling, turbulence and dilution effects is probably responsible for our observations. Additionally, during high leaf deposition periods, the presence of inhibitors resulted in no amplification for high copy number samples in the absence of an inhibition-releasing strategy, demonstrating the necessity to carefully consider inhibition in eDNA analysis. C1 [Jane, Stephen F.; Whiteley, Andrew R.] Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. [Wilcox, Taylor M.; Lowe, Winsor H.] Univ Montana, Div Biol Sci, Missoula, MT 59812 USA. [McKelvey, Kevin S.; Young, Michael K.; Schwartz, Michael K.] US Forest Serv, USDA, Rocky Mt Res Stn, Missoula, MT 59801 USA. [Letcher, Benjamin H.] US Geol Survey, Silvio O Conte Anadromous Fish Res Ctr, Turners Falls, MA 01376 USA. RP Jane, SF (reprint author), Univ Massachusetts, Dept Environm Conservat, Amherst, MA 01003 USA. EM coachman7777@yahoo.com RI Schwartz, Michael/C-3184-2014 OI Schwartz, Michael/0000-0003-3521-3367 FU NSF [DGE-1313190]; Rocky Mountain Research Station, USDA Forest Service [11-JV-11221635-081]; National Institute of Food and Agriculture, US Department of Agriculture; Massachusetts Agricultural Experiment Station; Environmental Conservation Department of the University of Massachusetts Amherst [MAS #14] FX We thank Todd Dubreuil for constructing the cage used in this study. Thanks to Keith Nislow and Jason Coombs at the USFS Northern Research Station for providing logistical support. Thanks to Kevin McGarigal, Jack Finn, Paul Sievert and Krzysztof Sakrejda for help with statistical analysis. Thanks to all who assisted with field work, including Matt Burak, Maili Page, Matt Cembrola and Alina Arnheim. Thanks to Nicole Sanford at the Northampton, MA DPW, and Gabrielle Kurth at the Amherst, MA DPW, for allowing access to their properties. T.M.W. is supported by a NSF Graduate Research Fellowship (Grant #DGE-1313190). This work was partially funded by the Rocky Mountain Research Station, USDA Forest Service through Joint Venture Agreement 11-JV-11221635-081. This work was also partially supported by the National Institute of Food and Agriculture, US Department of Agriculture, the Massachusetts Agricultural Experiment Station and the Environmental Conservation Department of the University of Massachusetts Amherst, under project number MAS #14. NR 53 TC 33 Z9 37 U1 16 U2 97 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1755-098X EI 1755-0998 J9 MOL ECOL RESOUR JI Mol. Ecol. Resour. PD JAN PY 2015 VL 15 IS 1 BP 216 EP 227 DI 10.1111/1755-0998.12285 PG 12 WC Biochemistry & Molecular Biology; Ecology; Evolutionary Biology SC Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology GA AX1HT UT WOS:000346699100020 PM 24890199 ER PT J AU Hatlestad, GJ Akhavan, NA Sunnadeniya, RM Elam, L Cargile, S Hembd, A Gonzalez, A McGrath, JM Lloyd, AM AF Hatlestad, Gregory J. Akhavan, Neda A. Sunnadeniya, Rasika M. Elam, Lee Cargile, Scott Hembd, Austin Gonzalez, Antonio McGrath, J. Mitchell Lloyd, Alan M. TI The beet Y locus encodes an anthocyanin MYB-like protein that activates the betalain red pigment pathway SO NATURE GENETICS LA English DT Article ID TRANSCRIPTION FACTORS; ARABIDOPSIS-THALIANA; TABLE BEET; IDENTIFICATION; BIOSYNTHESIS; EXPRESSION; EVOLUTION; GENES; CARYOPHYLLALES; SPECIFY AB Nearly all flowering plants produce red/violet anthocyanin pigments. Caryophyllales is the only order containing families that replace anthocyanins with unrelated red and yellow betalain pigments(1,2). Close biological correlation of pigmentation patterns suggested that betalains might be regulated by a conserved anthocyanin-regulating transcription factor complex consisting of a MYB, a bHLH and a WD repeat-containing protein (the MBW complex)(3). Here we show that a previously uncharacterized anthocyanin MYB-like protein, Beta vulgaris MYB1 (BvMYB1), regulates the betalain pathway in beets. Silencing BvMYB1 downregulates betalain biosynthetic genes and pigmentation, and overexpressing BvMYB1 upregulates them. However, unlike anthocyanin MYBs, BvMYB1 will not interact with bHLH members of heterologous anthocyanin MBW complexes because of identified nonconserved residues. BvMYB1 resides at the historic beet pigment-patterning locus, Y, required for red-fleshed beets(4). We show that Y and y express different levels of BvMYB1 transcripts. The co-option of a transcription factor regulating anthocyanin biosynthesis would be an important evolutionary event allowing betalains to largely functionally replace anthocyanins. C1 [Hatlestad, Gregory J.; Akhavan, Neda A.; Sunnadeniya, Rasika M.; Elam, Lee; Cargile, Scott; Hembd, Austin; Gonzalez, Antonio; Lloyd, Alan M.] Univ Texas Austin, Inst Cellular & Mol Biol, Dept Mol Biosci, Austin, TX 78712 USA. [McGrath, J. Mitchell] Michigan State Univ, USDA ARS, Sugarbeet & Bean Res Unit, E Lansing, MI 48824 USA. RP Lloyd, AM (reprint author), Univ Texas Austin, Inst Cellular & Mol Biol, Dept Mol Biosci, Austin, TX 78712 USA. EM amlloyd@utexas.edu FU US Department of Agriculture [2008-35301-19032]; US National Science Foundation [MCB-1122179] FX This work was supported by US Department of Agriculture grant 2008-35301-19032 and US National Science Foundation grant MCB-1122179. We thank E. Huq, S. Sung and V. Symonds for comments on the manuscript and discussion, I. Goldman (University of Wisconsin-Madison) for the W357B beet cultivar and K. Keller for pigment analysis. NR 29 TC 18 Z9 22 U1 5 U2 62 PU NATURE PUBLISHING GROUP PI NEW YORK PA 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA SN 1061-4036 EI 1546-1718 J9 NAT GENET JI Nature Genet. PD JAN PY 2015 VL 47 IS 1 BP 92 EP + DI 10.1038/ng.3163 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA AX5TM UT WOS:000346990400018 PM 25436858 ER PT J AU Starr, G Staudhammer, CL Loescher, HW Mitchell, R Whelan, A Hiers, JK O'Brien, JJ AF Starr, G. Staudhammer, C. L. Loescher, H. W. Mitchell, R. Whelan, A. Hiers, J. K. O'Brien, J. J. TI Time series analysis of forest carbon dynamics: recovery of Pinus palustris physiology following a prescribed fire SO NEW FORESTS LA English DT Article DE Longleaf pine; Net ecosystem exchange (NEE); Ecosystem respiration (Reco); Gross ecosystem exchange (GEE); Prescribed fire; ARIMA models ID CANADIAN BOREAL FOREST; LONGLEAF PINE; NORTH FLORIDA; TERRESTRIAL ECOSYSTEMS; STAND AGE; PRODUCTIVITY; VEGETATION; SOIL; SAVANNAS; IMPACT AB Frequency and intensity of fire determines the structure and regulates the function of savanna ecosystems worldwide, yet our understanding of prescribed fire impacts on carbon in these systems is rudimentary. We combined eddy covariance (EC) techniques and fuel consumption plots to examine the short-term response of longleaf pine forest carbon dynamics to one prescribed fire at the ends of an edaphic gradient (mesic and xeric sites). We also introduce novel (to the EC research community) statistical time-series approaches to quantify the drivers of carbon dynamics in these systems. We determined that our mesic site was a moderate sink of carbon (-157.7 +/- A 25.1 g C m(-2) year(-1)), while the xeric site was carbon neutral (5.9 +/- A 32.8 g C m(-2) year(-1)) during the study. The fire released 408 and 153 g C m(-2) year(-1) for the mesic and xeric sites, respectively. When loss associated with fire was combined with net ecosystem exchange rates, both sites became moderate carbon sources for the year. Analyses of assimilation and respiration parameters (e.g., maximum photosynthesis, quantum efficiency, and daytime ecosystem respiration) showed a positive trend over time pre-fire and a negative trend over time post-fire for maximum ecosystem CO2 uptake rates, and the opposite relationship for daytime ecosystem respiration rates. Within 30 days following fire, ecosystem physiological activity was statistically similar to pre-fire and appeared to be driven by the pine canopy. Our results suggest that prescribed fire (low intensity, high frequency) maintains the existing structure and function (in this case, carbon flux rates) because longleaf pine ecosystems have evolved with fire. This study, 1 year in length, provides a foundational understanding of the complex interaction between fire and carbon dynamics for longleaf pine ecosystems. Moreover, it provides a case study for applying time series analysis methods to EC data where there are complex relationships between ecosystem physiological activity and environmental drivers. However, to elicit a broader understanding of the complex interaction occurring between fire and carbon dynamics long- term studies are needed. C1 [Starr, G.; Staudhammer, C. L.; Whelan, A.] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Loescher, H. W.] Natl Ecol Observ Network NEON Inc, Boulder, CO 80301 USA. [Loescher, H. W.] Univ Colorado, Inst Arctic & Alpine Res INSTAAR, Boulder, CO 80309 USA. [Mitchell, R.] Jones Ecol Res Ctr, Newton, GA 39870 USA. [Hiers, J. K.] Nat Resource Sect Jackson Guard, Niceville, FL 32578 USA. [O'Brien, J. J.] US Forest Serv, Ctr Forest Disturbance Sci, USDA, Athens, GA 30602 USA. RP Starr, G (reprint author), Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. EM gstarr@ua.edu FU U. Alabama; Joseph Jones Ecological Research Center (JJERC) FX This research has been funded by U. Alabama and the Joseph Jones Ecological Research Center (JJERC). Authors also wish to acknowledge the superlative logistical support of the JJERC, especially J Bradley, B Shelton, and M Mazzacavaallo. HW Loescher acknowledges the National Science Foundation (DBI-0752017). We would also like to thank the two previous reviewers who helped increase the clarity of this manuscript through their suggestion on an early version of this paper. This paper would not have taken shape if it were not the guidance, lifetime work, and for many a conversation with J. Franklin. NR 63 TC 8 Z9 8 U1 4 U2 47 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0169-4286 EI 1573-5095 J9 NEW FOREST JI New For. PD JAN PY 2015 VL 46 IS 1 BP 63 EP 90 DI 10.1007/s11056-014-9447-3 PG 28 WC Forestry SC Forestry GA AX6MH UT WOS:000347035800005 ER PT J AU Hackenberg, M Gustafson, P Langridge, P Shi, BJ AF Hackenberg, Michael Gustafson, Perry Langridge, Peter Shi, Bu-Jun TI Differential expression of microRNAs and other small RNAs in barley between water and drought conditions SO PLANT BIOTECHNOLOGY JOURNAL LA English DT Article DE barley; drought; microRNA; small RNA; differential expression ID STRESS-RESPONSIVE MICRORNAS; GENOME-WIDE IDENTIFICATION; GENE-EXPRESSION; QUANTITATIVE-ANALYSIS; REPETITIVE ELEMENTS; REGULATED MICRORNAS; MIRNA TARGETS; ARABIDOPSIS; DEGRADOME; DATABASE AB Drought is a major constraint to crop production, and microRNAs (miRNAs) play an important role in plant drought tolerance. Analysis of miRNAs and other classes of small RNAs (sRNAs) in barley grown under water and drought conditions reveals that drought selectively regulates expression of miRNAs and other classes of sRNAs. Low-expressed miRNAs and all repeat-associated siRNAs (rasiRNAs) tended towards down-regulation, while tRNA-derived sRNAs (tsRNAs) had the tendency to be up-regulated, under drought. Antisense sRNAs (putative siRNAs) did not have such a tendency under drought. In drought-tolerant transgenic barley overexpressing DREB transcription factor, most of the low-expressed miRNAs were also down-regulated. In contrast, tsRNAs, rasiRNAs and other classes of sRNAs were not consistently expressed between the drought-treated and transgenic plants. The differential expression of miRNAs and siRNAs was further confirmed by Northern hybridization and quantitative real-time PCR (qRT-PCR). Targets of the drought-regulated miRNAs and siRNAs were predicted, identified by degradome libraries and confirmed by qRT-PCR. Their functions are diverse, but most are involved in transcriptional regulation. Our data provide insight into the expression profiles of miRNAs and other sRNAs, and their relationship under drought, thereby helping understand how miRNAs and sRNAs respond to drought stress in cereal crops. C1 [Hackenberg, Michael] Univ Granada, Dept Genet, Computat Genom & Bioinformat Grp, Granada, Spain. [Gustafson, Perry] Univ Missouri, USDA ARS, Columbia, MO 65211 USA. [Langridge, Peter; Shi, Bu-Jun] Univ Adelaide, Australian Ctr Plant Funct Genom, Urrbrae, SA, Australia. RP Shi, BJ (reprint author), Univ Adelaide, Australian Ctr Plant Funct Genom, Urrbrae, SA, Australia. EM bujun.shi@adelaide.edu.au RI Langridge, Peter/G-5864-2010; Hackenberg, Michael/A-2503-2009 OI Langridge, Peter/0000-0001-9494-400X; Hackenberg, Michael/0000-0003-2248-3114 NR 55 TC 20 Z9 22 U1 6 U2 45 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1467-7644 EI 1467-7652 J9 PLANT BIOTECHNOL J JI Plant Biotechnol. J. PD JAN PY 2015 VL 13 IS 1 BP 2 EP 13 DI 10.1111/pbi.12220 PG 12 WC Biotechnology & Applied Microbiology; Plant Sciences SC Biotechnology & Applied Microbiology; Plant Sciences GA AX4PW UT WOS:000346915000002 PM 24975557 ER PT J AU Davies, LJ Brown, CR Elling, AA AF Davies, Laura J. Brown, Charles R. Elling, Axel A. TI Calcium is involved in the R (Mc1) ((blb))-mediated hypersensitive response against Meloidogyne chitwoodi in potato SO PLANT CELL REPORTS LA English DT Article DE Meloidogyne chitwoodi; Potato (Solanum tuberosum); Reactive oxygen species (ROS); Root-knot nematode; Resistance; R-Mc1(blb) ID ROOT-KNOT NEMATODE; PLANT-DISEASE RESISTANCE; ARABIDOPSIS-THALIANA; HISTOLOGICAL CHARACTERIZATION; SOLANUM-BULBOCASTANUM; SIGNAL-TRANSDUCTION; FEEDING SITES; GLUTAMATE RECEPTORS; OXIDATIVE BURST; IMMUNE-RESPONSE AB Functional characterization of the Columbia root-knot nematode resistance gene R (Mc1 ( blb )) in potato revealed the R gene-mediated resistance is dependent on a hypersensitive response and involves calcium. The resistance (R) gene R (Mc1(blb)) confers resistance against the plant-parasitic nematode, Meloidogyne chitwoodi. Avirulent and virulent nematodes were used to functionally characterize the R (Mc1(blb))-mediated resistance mechanism in potato (Solanum tuberosum). Histological observations indicated a hypersensitive response (HR) occurred during avirulent nematode infection. This was confirmed by quantifying reactive oxygen species activity in response to avirulent and virulent M. chitwoodi. To gain an insight into the signal transduction pathways mediating the R (Mc1(blb))-induced HR, chemical inhibitors were utilized. Inhibiting Ca2+ channels caused a significant reduction in electrolyte leakage, an indicator of cell death. Labeling with a Ca2+-sensitive dye revealed high Ca2+ levels in the root cells surrounding avirulent nematodes. Furthermore, the calcium-dependent protein kinase (CDPK), StCDPK4 had a higher transcript level in R (Mc1(blb)) potato roots infected with avirulent nematodes in comparison to roots infected with virulent M. chitwoodi. The results of this study indicate Ca2+ plays a role in the R (Mc1(blb))-mediated resistance against M. chitwoodi in potato. C1 [Davies, Laura J.; Elling, Axel A.] Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. [Brown, Charles R.] ARS, Vegetable & Forage Crops Res Unit, USDA, Prosser, WA 99350 USA. RP Elling, AA (reprint author), Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA. EM elling@wsu.edu FU USDA; Washington State Potato Commission; Idaho Potato Commission; Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Washington State University, Pullman, WA, USA [WNP00744] FX This research was funded by grants from USDA, Washington State Potato Commission and Idaho Potato Commission to A.A.E. The authors thank Dr. Roger Innes for helpful discussions and sharing unpublished data, Dr. Helmut Kirchhoff for assistance with the ROS assay, and Drs. Linda Thomashow and Lee Hadwiger for critical reading of the manuscript. Drs. Christine Davitt and Valerie Lynch-Holm (Franceschi Microscopy and Imaging Center, Washington State University) assisted with cross sectioning and confocal microscopy. PPNS No. 0646, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences, Agricultural Research Center, Hatch Project No. WNP00744, Washington State University, Pullman, WA 99164-6430, USA. NR 72 TC 3 Z9 3 U1 6 U2 27 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0721-7714 EI 1432-203X J9 PLANT CELL REP JI Plant Cell Reports PD JAN PY 2015 VL 34 IS 1 BP 167 EP 177 DI 10.1007/s00299-014-1697-1 PG 11 WC Plant Sciences SC Plant Sciences GA AX9WS UT WOS:000347250600015 PM 25315813 ER PT J AU Jiao, YQ Vuong, TD Liu, Y Meinhardt, C Liu, Y Joshi, T Cregan, PB Xu, D Shannon, JG Nguyen, HT AF Jiao, Yongqing Vuong, Tri D. Liu, Yan Meinhardt, Clinton Liu, Yang Joshi, Trupti Cregan, Perry B. Xu, Dong Shannon, J. Grover Nguyen, Henry T. TI Identification and evaluation of quantitative trait loci underlying resistance to multiple HG types of soybean cyst nematode in soybean PI 437655 SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID HETERODERA-GLYCINES ICHINOHE; COPY NUMBER VARIATION; GENETIC-LINKAGE MAP; SCN RESISTANCE; RACES 1; POPULATIONS; QTL; RHG1; PI-88788 AB We performed QTL analysis for SCN resistance in PI 437655 in two mapping populations, characterized CNV of Rhg1 through whole-genome resequencing and evaluated the effects of QTL pyramiding to enhance resistance. Soybean cyst nematode (SCN, Heterodera glycines Ichinohe) is one of the most serious pests of soybean worldwide. PI 437655 has broader resistance to SCN HG types than PI 88788. The objectives of this study were to identify quantitative trait loci (QTL) underlying SCN resistance in PI 437655, and to evaluate the QTL for their contribution to SCN resistance. Two F-6:7 recombinant inbred line populations, derived from cv. Williams 82 x PI 437655 and cv. Hutcheson x PI 437655 crosses, were evaluated for resistance to SCN HG types 1.2.5.7 (PA2), 0 (PA3), 1.3.5.6.7 (PA14), and 1.2.3.4.5.6.7 (LY2). The 1,536 SNP array was used to genotype the mapping populations and construct genetic linkage maps. Two significant QTL were consistently mapped on chromosomes (Chr.) 18 and 20 in these two populations. One QTL on Chr. 18, which corresponds to the known Rhg1 locus, contributed resistance to SCN HG types 1.2.5.7, 0, 1.3.5.6.7, and 1.2.3.4.5.6.7 (PA2, PA3, PA14, and LY2, respectively). Copy number variation (CNV) analysis by whole-genome resequencing showed that PI 437655 and PI 88788 had similar CNV at the Rhg1 locus. The QTL on Chr. 20 contributed resistance to SCN HG types 1.3.5.6.7 (PA14) and 1.2.3.4.5.6.7 (LY2). Evaluation of both QTL showed that pyramiding of Rhg1 and the QTL on Chr. 20 significantly improved the resistance to SCN HG types 1.3.5.6.7 (PA14) and 1.2.3.4.5.6.7 (LY2) in both populations. Our studies provided useful information for deploying PI 437655 as a donor for SCN resistance in soybean breeding through marker-assisted selection. C1 [Jiao, Yongqing; Vuong, Tri D.; Liu, Yan; Meinhardt, Clinton; Nguyen, Henry T.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Jiao, Yongqing; Vuong, Tri D.; Liu, Yan; Meinhardt, Clinton; Nguyen, Henry T.] Univ Missouri, NCSB, Columbia, MO 65211 USA. [Liu, Yan; Joshi, Trupti; Xu, Dong] Univ Missouri, Dept Comp Sci, Inst Informat, Columbia, MO 65211 USA. [Liu, Yang; Joshi, Trupti; Xu, Dong] Univ Missouri, Christopher S Bond Life Sci Ctr, Columbia, MO 65211 USA. [Cregan, Perry B.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Shannon, J. Grover] Univ Missouri, Delta Ctr, Div Plant Sci, Portageville, MO 63873 USA. [Shannon, J. Grover] Univ Missouri, Delta Ctr, NCSB, Portageville, MO 63873 USA. RP Jiao, YQ (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM yongqing.jiao@gmail.com; nguyenhenry@missouri.edu FU United Soybean Board; Missouri Soybean Merchandizing Council FX The authors would like to thank three anonymous reviewers for their constructive comments. The authors also would like to thank Theresa Musket, Cuilan Liu, Meghan Coburn, Michelle Lambert, and Suhas Kadam, Division of Plant Sciences, University of Missouri, for their technical assistance and suggestions on the manuscript. This research was supported by the United Soybean Board and Missouri Soybean Merchandizing Council. NR 42 TC 6 Z9 7 U1 1 U2 29 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JAN PY 2015 VL 128 IS 1 BP 15 EP 23 DI 10.1007/s00122-014-2409-5 PG 9 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AX9XE UT WOS:000347251700002 PM 25316311 ER PT J AU Bo, KL Ma, Z Chen, JF Weng, YQ AF Bo, Kailiang Ma, Zheng Chen, Jinfeng Weng, Yiqun TI Molecular mapping reveals structural rearrangements and quantitative trait loci underlying traits with local adaptation in semi-wild Xishuangbanna cucumber (Cucumis sativus L. var. xishuangbannanesis Qi et Yuan) SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID POWDERY MILDEW RESISTANCE; RECOMBINANT INBRED LINES; MELON C. MELO; QTL ANALYSIS; FRUIT SHAPE; CROP DOMESTICATION; DNA-SEQUENCES; GENETIC-BASIS; GENOME; EVOLUTION AB Comparative genetic mapping revealed the origin of Xishuangbanna cucumber through diversification selection after domestication. QTL mapping provided insights into the genetic basis of traits under diversification selection during crop evolution. The Xishuangbanna cucumber, Cucumis sativus L. var. xishuangbannanesis Qi et Yuan (XIS), is a semi-wild landrace from the tropical southwest China with some unique traits that are very useful for cucumber breeding, such as tolerance to low light, large fruit size, heavy fruit weight, and orange flesh color in mature fruits. In this study, using 124 recombinant inbred lines (RILs) derived from the cross of the XIS cucumber with a cultivated cucumber inbred line, we developed a linkage map with 269 microsatellite (or simple sequence repeat) markers which covered 705.9 cM in seven linkage groups. Comparative analysis of orders of common marker loci or marker-anchored draft genome scaffolds among the wild (C. sativus var. hardwickii), semi-wild, and cultivated cucumber genetic maps revealed that the XIS cucumber shares major chromosomal rearrangements in chromosomes 4, 5, and 7 between the wild and cultivated cucumbers suggesting that the XIS cucumber originated through diversifying selection after cucumber domestication. Several XIS-specific minor structural changes were identified in chromosomes 1 and 6. QTL mapping with the 124 RILs in four environments identified 13 QTLs for domestication and diversifying selection-related traits including 2 for first female flowering time (fft1.1, fft6.1), 5 for mature fruit length (fl1.1, fl3.1, fl4.1, fl6.1, and fl7.1), 3 for fruit diameter (fd1.1, fd4.1, and fd6.1), and 3 for fruit weight (fw2.1, fw4.1, and fw6.1). Six of the 12 QTLs were consistently detected in all four environments. Among the 13 QTLs, fft1.1, fl1.1, fl3.1, fl7.1, fd4.1, and fw6.1 were major-effect QTLs for respective traits with each explaining at least 10 % of the observed phenotypic variations. Results from this study provide insights into the cytological and genetic basis of crop evolution leading to the XIS cucumber. The molecular markers associated with the QTLs should be useful in exploring the XIS cucumber genetic resources for cucumber breeding. C1 [Bo, Kailiang; Ma, Zheng; Chen, Jinfeng] Nanjing Agr Univ, Hort Coll, Nanjing 210095, Jiangsu, Peoples R China. [Bo, Kailiang; Weng, Yiqun] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Weng, Yiqun] USDA ARS, Vegetable Crops Res Unit, Madison, WI 53706 USA. RP Chen, JF (reprint author), Nanjing Agr Univ, Hort Coll, Nanjing 210095, Jiangsu, Peoples R China. EM jfchen@njau.edu.cn; yiqun.weng@ars.usda.gov FU China Scholarship Council; US Department of Agriculture Current Research Information System Project [3655-21000-048-00D]; US Department of Agriculture Specialty Crop Research Initiative grant [2011-51181-30661]; Natural Science Foundation of China [30972007, 31272174]; '973' Program from the National Basic Research Program of China [2012CB113904]; '863' project [2012AA100102] FX The authors thank Kristin Haider for technical assistance. KB's work in YW's laboratory was partially funded by the China Scholarship Council. This research was supported by the US Department of Agriculture Current Research Information System Project 3655-21000-048-00D and a US Department of Agriculture Specialty Crop Research Initiative grant (project number 2011-51181-30661) to Y.W. Work pertinent to this project in JC Lab was supported by the Natural Science Foundation of China (30972007 and 31272174) and the '973' Program (2012CB113904) from the National Basic Research Program of China and '863' project (2012AA100102). NR 60 TC 5 Z9 7 U1 7 U2 47 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JAN PY 2015 VL 128 IS 1 BP 25 EP 39 DI 10.1007/s00122-014-2410-z PG 15 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AX9XE UT WOS:000347251700003 PM 25358412 ER PT J AU Dhanapal, AP Ray, JD Singh, SK Hoyos-Villegas, V Smith, JR Purcell, LC King, CA Cregan, PB Song, QJ Fritschi, FB AF Dhanapal, Arun Prabhu Ray, Jeffery D. Singh, Shardendu K. Hoyos-Villegas, Valerio Smith, James R. Purcell, Larry C. King, C. Andy Cregan, Perry B. Song, Qijian Fritschi, Felix B. TI Genome-wide association study (GWAS) of carbon isotope ratio (delta C-13) in diverse soybean [Glycine max (L.) Merr.] genotypes SO THEORETICAL AND APPLIED GENETICS LA English DT Article DE Carbon isotope ratio; Genome wide association studies (GWAS); SNPs; Water use efficiency (WUE) ID WATER-USE EFFICIENCY; QUANTITATIVE TRAIT LOCI; ORYZA-SATIVA L.; POPULATION-STRUCTURE; TRANSPIRATION EFFICIENCY; LINKAGE DISEQUILIBRIUM; GENETIC DIVERSITY; DROUGHT TOLERANCE; COMPLEX TRAITS; STOMATAL CONDUCTANCE AB Using genome-wide association studies, 39 SNP markers likely tagging 21 different loci for carbon isotope ratio (delta (13) C) were identified in soybean. Water deficit stress is a major factor limiting soybean [Glycine max (L.) Merr.] yield. Soybean genotypes with improved water use efficiency (WUE) may be used to develop cultivars with increased yield under drought. A collection of 373 diverse soybean genotypes was grown in four environments (2 years and two locations) and characterized for carbon isotope ratio (delta C-13) as a surrogate measure of WUE. Population structure was assessed based on 12,347 single nucleotide polymorphisms (SNPs), and genome-wide association studies (GWAS) were conducted to identify SNPs associated with delta C-13. Across all four environments, delta C-13 ranged from a minimum of -30.55 aEuro degrees to a maximum of -27.74 aEuro degrees. Although delta C-13 values were significantly different between the two locations in both years, results were consistent among genotypes across years and locations. Diversity analysis indicated that eight subpopulations could contain all individuals and revealed that within-subpopulation diversity, rather than among-subpopulation diversity, explained most (80 %) of the diversity among the 373 genotypes. A total of 39 SNPs that showed a significant association with delta C-13 in at least two environments or for the average across all environments were identified by GWAS. Fifteen of these SNPs were located within a gene. The 39 SNPs likely tagged 21 different loci and demonstrated that markers for delta C-13 can be identified in soybean using GWAS. Further research is necessary to confirm the marker associations identified and to evaluate their usefulness for selecting genotypes with increased WUE. C1 [Dhanapal, Arun Prabhu; Fritschi, Felix B.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Ray, Jeffery D.; Smith, James R.] USDA ARS, Crop Genet Res Unit, Stoneville, MS 38776 USA. [Singh, Shardendu K.] USDA ARS, Crop Syst & Global Change Lab, Beltsville, MD 20705 USA. [Hoyos-Villegas, Valerio] Michigan State Univ, Dept Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Purcell, Larry C.; King, C. Andy] Univ Arkansas, Dept Crop Soil & Environm Sci, Fayetteville, AR 72704 USA. [Cregan, Perry B.; Song, Qijian] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. RP Fritschi, FB (reprint author), Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. EM fritschif@missouri.edu RI Dhanapal, Arun Prabhu/F-5030-2012 OI Dhanapal, Arun Prabhu/0000-0002-9686-7260 FU United States Department of Agriculture-Agriculture Research Service (USDA-ARS) [6402-21220-010-00D]; United Soybean Board [9274, 1274] FX This work was supported by United States Department of Agriculture-Agriculture Research Service (USDA-ARS) project number 6402-21220-010-00D and United Soybean Board project numbers 9274 and 1274. We appreciate the assistance of Dr. Randall Nelson, curator of the USDA-ARS Germplasm Collection in selecting the genotypes evaluated in this study. NR 96 TC 17 Z9 17 U1 1 U2 36 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JAN PY 2015 VL 128 IS 1 BP 73 EP 91 DI 10.1007/s00122-014-2413-9 PG 19 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AX9XE UT WOS:000347251700006 PM 25367378 ER PT J AU Isidro, J Jannink, JL Akdemir, D Poland, J Heslot, N Sorrells, ME AF Isidro, Julio Jannink, Jean-Luc Akdemir, Deniz Poland, Jesse Heslot, Nicolas Sorrells, Mark E. TI Training set optimization under population structure in genomic selection SO THEORETICAL AND APPLIED GENETICS LA English DT Article ID GENETIC-RELATIONSHIP INFORMATION; DENSITY MARKER PANELS; BREEDING VALUES; COMPLEX TRAITS; DAIRY-CATTLE; GENOTYPING STRATEGIES; GENOMEWIDE SELECTION; QUANTITATIVE TRAITS; PREDICTION MODELS; RIDGE-REGRESSION AB Population structure must be evaluated before optimization of the training set population. Maximizing the phenotypic variance captured by the training set is important for optimal performance. The optimization of the training set (TRS) in genomic selection has received much interest in both animal and plant breeding, because it is critical to the accuracy of the prediction models. In this study, five different TRS sampling algorithms, stratified sampling, mean of the coefficient of determination (CDmean), mean of predictor error variance (PEVmean), stratified CDmean (StratCDmean) and random sampling, were evaluated for prediction accuracy in the presence of different levels of population structure. In the presence of population structure, the most phenotypic variation captured by a sampling method in the TRS is desirable. The wheat dataset showed mild population structure, and CDmean and stratified CDmean methods showed the highest accuracies for all the traits except for test weight and heading date. The rice dataset had strong population structure and the approach based on stratified sampling showed the highest accuracies for all traits. In general, CDmean minimized the relationship between genotypes in the TRS, maximizing the relationship between TRS and the test set. This makes it suitable as an optimization criterion for long-term selection. Our results indicated that the best selection criterion used to optimize the TRS seems to depend on the interaction of trait architecture and population structure. C1 [Isidro, Julio; Jannink, Jean-Luc; Akdemir, Deniz; Sorrells, Mark E.] Cornell Univ, Ithaca, NY 14850 USA. [Poland, Jesse] Kansas State Univ, USDA ARS, Hard Winter Wheat Genet Res Unit, Manhattan, KS 66506 USA. [Poland, Jesse] Kansas State Univ, Dept Agron, Manhattan, KS 66506 USA. [Heslot, Nicolas] Limagrain Europe, F-63720 Chappes, France. RP Isidro, J (reprint author), Cornell Univ, Ithaca, NY 14850 USA. EM ji66@cornell.edu OI Poland, Jesse/0000-0002-7856-1399; Isidro-Sanchez, Julio/0000-0002-9044-3221 FU Regional Ministry of Economy, Innovation and Science of Andalusia, Spain; National Research Initiative Competitive Grant from the USDA National Institute of Food and Agriculture [2011-68002-30029]; Hatch project [149-449] FX The Regional Ministry of Economy, Innovation and Science of Andalusia, Spain provided financial support for Julio Isidro Sanchez. The authors would like to than researchers and institutions that contributed to the development of the rice diversity panel. In addition, the authors would like to express gratitude to Dr. R. Rincent who gently provided us the script model to optimize the training set based on CDmean. We also thank to all collaborators involved in conducting the trials. This research was supported in part by the National Research Initiative Competitive Grant 2011-68002-30029 (Triticeae-CAP) from the USDA National Institute of Food and Agriculture and by Hatch project 149-449. NR 61 TC 25 Z9 25 U1 12 U2 60 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0040-5752 EI 1432-2242 J9 THEOR APPL GENET JI Theor. Appl. Genet. PD JAN PY 2015 VL 128 IS 1 BP 145 EP 158 DI 10.1007/s00122-014-2418-4 PG 14 WC Agronomy; Plant Sciences; Genetics & Heredity; Horticulture SC Agriculture; Plant Sciences; Genetics & Heredity GA AX9XE UT WOS:000347251700011 PM 25367380 ER PT J AU Progar, RA Kruse, JJ Lundquist, JE Zogas, KP Rinella, MJ AF Progar, R. A. Kruse, J. J. Lundquist, J. E. Zogas, K. P. Rinella, M. J. TI An entomopathogenic fungus and nematode prove ineffective for biocontrol of an invasive leaf miner Profenusa thomsoni in Alaska SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE Profenusa thomsoni; Beauveria bassiana; Steinernema carpocapsae; biological control; invasive insect ID STEINERNEMA-CARPOCAPSAE RHABDITIDA; LEAFMINING SAWFLIES HYMENOPTERA; BEAUVERIA-BASSIANA; METARHIZIUM-ANISOPLIAE; RADIAL GROWTH; HETERORHABDITIS-BACTERIOPHORA; ENTOMOGENOUS NEMATODES; DIPTERA TEPHRITIDAE; EMAMECTIN BENZOATE; BIOLOGICAL-CONTROL AB A non-native invasive sawfly, the amber-marked birch leaf miner Profenusa thomsoni (Konow), was first detected in south-central Alaska in 1996 and is now widely distributed throughout urban and wild birch trees in Alaska. Impacts have been considered primarily aesthetic because leaf miners cause leaves of birch trees (Betula spp.) to senesce prematurely, but the leaf miners likely also reduce birch vigour and thereby increase susceptibility to diseases and other insects. We tested the ability of commercially available biological control agents to control P. thomsoni. The entomopathogenic fungus Beauveria bassiana (Bals.-Criv.) Vuillemin GHA strain and the entomopathogenic nematode Steinernema carpocapsae (Weiser) were applied in aqueous suspension to the soil/litter surface beneath infested birch trees in Alaska at one site in 2007 and 2008 and two sites in 2010. There was no evidence the fungus or nematode controlled P. thomsoni. Instead, there was evidence the fungus increased the density of this pest insect at two sites, likely by reducing its predators. As tested, B. bassiana and S. carpocapsae do not appear effective as biological controls of P. thomsoni. C1 [Progar, R. A.] US Forest Serv, USDA, Pacific NW Res Stn, La Grande, OR 97850 USA. [Kruse, J. J.] US Forest Serv, USDA, State & Private Forestry R10, Forest Hlth Protect, Fairbanks, AK USA. [Lundquist, J. E.; Zogas, K. P.] US Forest Serv, USDA, State & Private Forestry R10, Forest Hlth Protect, Anchorage, AK USA. [Rinella, M. J.] USDA ARS, Miles City, MT USA. RP Progar, RA (reprint author), US Forest Serv, USDA, Pacific NW Res Stn, La Grande, OR 97850 USA. EM rprogar@fs.fed.us FU US Department of Agriculture Region 10, Forest Health Protection FX This research was funded by US Department of Agriculture Region 10, Forest Health Protection. NR 56 TC 0 Z9 0 U1 3 U2 15 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PY 2015 VL 25 IS 4 BP 373 EP 382 DI 10.1080/09583157.2014.977224 PG 10 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AX0MN UT WOS:000346646500002 ER PT J AU Olanya, OM Taylor, J Ukuku, DO Malik, NSA AF Olanya, O. Modesto Taylor, Janysha Ukuku, Dike O. Malik, Nasir S. A. TI Inactivation of Salmonella serovars by Pseudomonas chlororaphis and Pseudomonas fluorescens strains on tomatoes SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE microflora; Salmonella serovars; Pseudomonas chlororaphis; Pseudomonas fluorescens; storage time; tomatoes ID FRESH-CUT PIECES; LISTERIA-MONOCYTOGENES; NATIVE MICROFLORA; EMERGING TECHNOLOGIES; IN-VITRO; PATHOGENS; ENTERICA; TYPHIMURIUM; BACTERIA; RHIZOSPHERE AB Salmonella enterica and its serovars have been associated with pathogen contamination of tomatoes with numerous outbreaks of salmonellosis. To improve food safety, pathogen control is of immediate concern. The aim of this research was to assess the populations of natural microflora (aerobic mesophilic bacteria, lactic acid bacteria, yeasts and moulds and Pseudomonas species) on tomatoes, and evaluate the efficacy of Pseudomonas fluorescens (Pf) and Pseudomonas chlororaphis (Pc) for inactivation of Salmonella on tomatoes. Microflora were determined on sanitised and unsanitised produce and enumerated on Plate Count Agar, de Man, Rogosa and Sharpe medium, Potato Dextrose Agar and Pseudomonas Agar F media. The efficacy of Pc and Pf for inactivation of S. enterica serovars Montevideo, Typhimurium and Poona was determined on spot-inoculated tomato stem scars. The effects of storage time on bacterial populations were also investigated. On unsanitised tomatoes, lactic acid bacteria, Pseudomonas sp., aerobic mesophilic bacteria and yeasts and moulds ranged from 3.31-4.84, 3.93-4.77, 4.09-4.80 and 3.83-4.67 log CFU/g of produce, respectively. The microflora were similar at 0 and 24 storage hours on sanitised produce. The suppression of Salmonella Montevideo by P. chlororaphis and P. fluorescens on tomatoes ranged from 0.51 to 2.00 log CFU/g of produce. On Salmonella Montevideo and S. Typhimurium, the suppressive effects ranged from 0.51 to 0.95 and 0.46 to 2.00 log CFU/g of produce, respectively. The pathogen suppressive effects may be attributed to competition ability of Pseudomonas relative to Salmonella strains. Pseudomonas strains may be effective against Salmonella strains as a post-harvest application, but strain synergy is required to optimise pathogen reductions. C1 [Olanya, O. Modesto; Taylor, Janysha; Ukuku, Dike O.] USDA ARS, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, Wyndmoor, PA 19038 USA. [Malik, Nasir S. A.] USDA ARS, Eastern Reg Res Ctr, Mol Detect Foodborne Pathogens Res Unit, Wyndmoor, PA USA. RP Olanya, OM (reprint author), USDA ARS, Eastern Reg Res Ctr, Food Safety & Intervent Technol Res Unit, Wyndmoor, PA 19038 USA. EM modesto.olanya@ars.usda.gov NR 29 TC 3 Z9 3 U1 3 U2 11 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PY 2015 VL 25 IS 4 BP 399 EP 413 DI 10.1080/09583157.2014.982513 PG 15 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AX0MN UT WOS:000346646500004 ER PT J AU Ramakodi, MP Singh, B Wells, JD Guerrero, F Ray, DA AF Ramakodi, Meganathan P. Singh, Baneshwar Wells, Jeffrey D. Guerrero, Felix Ray, David A. TI A 454 sequencing approach to dipteran mitochondrial genome research SO GENOMICS LA English DT Article DE Diptera; Mitochondrial genomes; 454 sequencing; Forensic entomology; Evolution; Phylogenetic analyses ID TRANSFER-RNA GENES; DROSOPHILA-MELANOGASTER; MICROSATELLITE LOCI; MOLECULAR PHYLOGENETICS; NUCLEOTIDE-SEQUENCE; BLOWFLIES DIPTERA; HIGH-THROUGHPUT; CODON USAGE; DNA; FLY AB The availability of complete mitochondrial genome (mtgenome) data for Diptera, one of the largest metazoan orders, in public databases is limited. The advent of high throughput sequencing technology provides the potential to generate mtgenomes for many species affordably and quickly. However, these technologies need to be validated for dipterans as the members of this clade play important economic and research roles. Illumina and 454 sequencing platforms are widely used in genomic research involving non-model organisms. The Illumina platform has already been utilized for generating mitochondrial genomes without using conventional long range PCR for insects whereas the power of 454 sequencing for generating mitochondrial genome drafts without PCR has not yet been validated for insects. Thus, this study examines the utility of 454 sequencing approach for dipteran mtgenomic research. We generated complete or nearly complete mitochondrial genomes for Cochliomyia hominivorax, Haematobia irritans, Phormia regina and Sarcophaga crassipalpis using a 454 sequencing approach. Comparisons between newly obtained and existing assemblies for C. hominivorax and H. irritans revealed no major discrepancies and verified the utility of 454 sequencing for dipteran mitochondrial genomes. We also report the complete mitochondrial sequences for two forensically important flies, P. regina and S. crassipalpis, which could be used to provide useful information to legal personnel. Comparative analyses revealed that dipterans follow similar codon usage and nucleotide biases that could be due to mutational and selection pressures. This study illustrates the utility of 454 sequencing to obtain complete mitochondrial genomes for dipterans without the aid of conventional molecular techniques such as PCR and cloning and validates this method of mtgenome sequencing in arthropods. (C) 2014 Elsevier Inc. All rights reserved. C1 [Ramakodi, Meganathan P.; Ray, David A.] Mississippi State Univ, Inst Genom Biocomp & Biotechnol, Mississippi State, MS 39762 USA. [Ramakodi, Meganathan P.; Ray, David A.] Mississippi State Univ, Dept Biochem Mol Biol Entomol & Plant Pathol, Mississippi State, MS 39762 USA. [Singh, Baneshwar; Wells, Jeffrey D.] W Virginia Univ, Dept Biol, Morgantown, WV 26506 USA. [Guerrero, Felix] USDA ARS, Knipling Bushland US Livestock Insect Res Lab, Kerrville, TX 78028 USA. RP Ray, DA (reprint author), Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA. EM david.4.ray@gmail.com FU Mississippi Agricultural and Forestry Experiment Station (MAFES); National Science Foundation [MCB-0841821, DEB-1020865, MCB-1052500] FX This research was made possible by funding from Special Research Initiative grants from the Mississippi Agricultural and Forestry Experiment Station (MAFES) and by the National Science Foundation (MCB-0841821, DEB-1020865, and MCB-1052500) to DAR. USDA is an equal opportunity employer. We thank T. Glenn and the staff of the Georgia Genomics Facility at the University of Georgia for their aid in sequence generation. We also thank two independent reviewers of this MS for their thoughts. NR 66 TC 8 Z9 9 U1 0 U2 42 PU ACADEMIC PRESS INC ELSEVIER SCIENCE PI SAN DIEGO PA 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA SN 0888-7543 EI 1089-8646 J9 GENOMICS JI Genomics PD JAN PY 2015 VL 105 IS 1 BP 53 EP 60 DI 10.1016/j.ygeno.2014.10.014 PG 8 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AX0LM UT WOS:000346643900007 PM 25451744 ER PT J AU Liu, HR Jiang, ZH Fei, BH Hse, CY Sun, ZJ AF Liu, Huanrong Jiang, Zehui Fei, Benhua Hse, Chungyun Sun, Zhengjun TI Tensile behaviour and fracture mechanism of moso bamboo (Phyllostachys pubescens) SO HOLZFORSCHUNG LA English DT Article DE fracture mechanics; hierarchical structure; moso bamboo; tensile behaviour ID BIOLOGICAL-MATERIALS; TOUGHNESS; STRENGTH; NACRE; BONE AB The present work is aiming at the elucidation of the tensile behaviour and fracture performance of moso bamboo (Phyllostachys pubescens Mazei ex H. de Lebaie) by means of digital speckle correlation method (DSCM) and microscopic techniques. Results indicated that fibres play a major role in longitudinal tension and impeding crack radial propagation. Hybrid I-II failure mode was observed, i.e., crack opening (in tensile stress) and shear sliding (in shear stress). According to microscopic fracture characteristics, fibres extraction and stretching, filament formation in parenchyma with fibres bridging, interface debonding and the helix fracture of fibres happened in tension, which created more interfaces and dissipated more energy. The graded composite structure of bamboo provides intrinsic and extrinsic toughening mechanisms which contribute to improved toughness and physical properties. C1 [Liu, Huanrong; Jiang, Zehui; Fei, Benhua; Sun, Zhengjun] Int Ctr Bamboo & Rattan, Dept Biomat, Beijing 100102, Peoples R China. [Hse, Chungyun] USDA, Alexandria Forestry Ctr, Forest Serv, Pineville, LA 71360 USA. RP Jiang, ZH (reprint author), Int Ctr Bamboo & Rattan, Dept Biomat, 8 Futong Eastern St, Beijing 100102, Peoples R China. EM Jiangzehui@icbr.ac.cn FU 12th Five Years Key Technology R&D Program of China [2012BAD54G01, 2012BAD23B01] FX We acknowledge the support from 12th Five Years Key Technology R&D Program of China (2012BAD54G01) and (2012BAD23B01). NR 36 TC 1 Z9 1 U1 4 U2 17 PU WALTER DE GRUYTER GMBH PI BERLIN PA GENTHINER STRASSE 13, D-10785 BERLIN, GERMANY SN 0018-3830 EI 1437-434X J9 HOLZFORSCHUNG JI Holzforschung PD JAN PY 2015 VL 69 IS 1 BP 47 EP 52 DI 10.1515/hf-2013-0220 PG 6 WC Forestry; Materials Science, Paper & Wood SC Forestry; Materials Science GA AX2HR UT WOS:000346764600007 ER PT J AU Christen, JA Skoda, SR Heng-Moss, TM Lee, DJ Foster, JE AF Christen, Joan A. Skoda, Steven R. Heng-Moss, Tiffany M. Lee, Donald J. Foster, John E. TI Sequence-characterized amplified regions that differentiate New World screwworms from other potential wound-inhabiting flies SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Cochliomyia hominivorax; molecular markers; randomly amplified polymorphic DNA polymerase chain reaction; sterile insect technique ID COCHLIOMYIA-HOMINIVORAX; MITOCHONDRIAL-DNA; MOLECULAR MARKERS; CLUSTAL-W; CALLIPHORIDAE; DIPTERA; IDENTIFICATION; MACELLARIA; AMERICA; PROGRAM AB New World screwworms, Cochliomyia hominivorax (Coquerel, 1858), were once devastating pests of warm-blooded animals in the United States before they were successfully eradicated using the sterile insect technique. Guarding against the introduction of screwworms to North America or any other screwworm-free area relies on rapid, reliable identification of suspected cases. In the current study, the DNA from excised markers generated by randomly amplified polymorphic DNA polymerase chain reaction was used as the basis to generate 2 species-specific sequence-characterized amplified region molecular markers. Resulting primer pairs, named CR92A1 and J1A2 (each with forward and reverse components), produced amplicons of 852 and 848 base pairs, respectively. The 2 primer pairs successfully discriminated between C. hominivorax, Cochliomyia macellaria (Fabricius, 1775), 8 other species of blowflies, 3 noncalliphorid dipterans, and 1 nondipteran outlier. These primers may become important tools for veterinary laboratories and the screwworm eradication and exclusion program for rapid identification or verification of suspicious larval samples in presumed outbreaks. C1 [Christen, Joan A.; Heng-Moss, Tiffany M.; Foster, John E.] Univ Nebraska, Dept Entomol, Genet Lab, Lincoln, NE 68583 USA. [Lee, Donald J.] Univ Nebraska, Dept Agron & Hort, Lincoln, NE USA. [Skoda, Steven R.] ARS, USDA, Knipling Bushland US Livestock Insects Res Lab, Screwworm Res Unit, Kerrville, TX USA. RP Skoda, SR (reprint author), ARS, USDA, KBUSLIRL, SRU, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM steve.skoda@ars.usda.gov NR 34 TC 0 Z9 0 U1 1 U2 2 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD JAN PY 2015 VL 27 IS 1 BP 25 EP 30 DI 10.1177/1040638714555897 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA AX0PD UT WOS:000346653200003 PM 25387845 ER PT J AU Snider, DB Gardner, DR Janke, BH Ensley, SM AF Snider, Douglas B. Gardner, Dale R. Janke, Bruce H. Ensley, Steven M. TI Pine needle abortion biomarker detected in bovine fetal fluids SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Abortion; cattle; isocupressic acid; pine needle; tetrahydroagathic acid ID PONDEROSA PINE; ISOCUPRESSIC ACID; ABORTIFACIENT COMPOUNDS; BEEF-COWS; CATTLE; INGESTION; METABOLISM; COMPOUND AB Pine needle abortion is a naturally occurring condition in free-range cattle caused by the consumption of pine needles from select species of cypress, juniper, pine, and spruce trees. Confirmatory diagnosis of pine needle abortion has previously relied on a combined case history of pine needle consumption and detection of isocupressic acid in a sample from the dam. Stable metabolites of isocupressic acid include agathic acid, dihydroagathic acid, and tetrahydroagathic acid, which have been shown to be present in the serum of mature animals for a few days following consumption of pine needles. As maternal serum is infrequently submitted for diagnosis of cattle abortions, a diagnostic assay capable of confirming isocupressic acid exposure in other matrices would be desirable. To the authors' knowledge, no previous investigations have indicated whether these stable metabolites of isocupressic acid cross the placenta or are detectable in fetal tissues. Therefore, the presence of agathic acid, dihydroagathic acid, and tetrahydroagathic acid was evaluated using gas chromatography-mass spectroscopy on fetal thoracic fluid and stomach contents collected from 2 aborted bovine fetuses with a recent herd history of pine needle consumption by the dams and a subsequent abortion outbreak in the herd. Only tetrahydroagathic acid was detected in the fetal thoracic fluid and fetal stomach contents. The current study encourages diagnosticians to collect fetal thoracic fluids to permit the detection of tetrahydroagathic acid in cases of suspected pine needle abortion. C1 [Snider, Douglas B.; Janke, Bruce H.; Ensley, Steven M.] Iowa State Univ, Coll Vet Med, Vet Diagnost Lab, Dept Vet Diagnost & Prod Anim Med, Ames, IA 50011 USA. [Gardner, Dale R.] ARS, USDA, Poisonous Plant Res Lab, Logan, UT USA. RP Snider, DB (reprint author), Iowa State Univ, Coll Vet Med, Vet Diagnost Lab, Toxicol & Nutr Sect, 1600 South 16th St, Ames, IA 50011 USA. EM dbsnider@alumni.iastate.edu NR 24 TC 0 Z9 0 U1 1 U2 4 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD JAN PY 2015 VL 27 IS 1 BP 74 EP 79 DI 10.1177/1040638714554443 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA AX0PD UT WOS:000346653200010 PM 25428187 ER PT J AU Fulton, RW Herd, HR Sorensen, NJ Confer, AW Ritchey, JW Ridpath, JF Burge, LJ AF Fulton, Robert W. Herd, Heather R. Sorensen, Nicholas J. Confer, Anthony W. Ritchey, Jerry W. Ridpath, Julia F. Burge, Lurinda J. TI Enteric disease in postweaned beef calves associated with Bovine coronavirus clade 2 SO JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION LA English DT Article DE Beef cattle; Bovine coronavirus; enteric disease ID WINTER DYSENTERY; FEEDLOT; CATTLE; INFECTIONS AB Bovine coronavirus (BoCV; Betacoronavirus 1) infections are associated with varied clinical presentations including neonatal diarrhea, winter dysentery in dairy cattle, and respiratory disease in various ages of cattle. The current report presents information on BoCV infections associated with enteric disease of postweaned beef cattle in Oklahoma. In 3 separate accessions from a single herd, 1 in 2012 and 2 in 2013, calves were observed with bloody diarrhea. One calf in 2012 died and was necropsied, and 2 calves from this herd died in 2013 and were necropsied. A third calf from another herd died and was necropsied. The gross and histologic diagnosis was acute, hemorrhagic colitis in all 4 cattle. Colonic tissues from all 4 animals were positive by fluorescent antibody testing and/or immunohistochemical staining for BoCV antigen. Bovine coronavirus was isolated in human rectal tumor cells from swabs of colon surfaces of all animals. The genomic information from a region of the S envelope region revealed BoCV clade 2. Detection of BoCV clade 2 in beef cattle in Oklahoma is consistent with recovery of BoCV clade 2 from the respiratory tract of postweaned beef calves that had respiratory disease signs or were healthy. Further investigations on the ecology of BoCV in cattle are important, as BoCV may be an emerging disease beyond the initial descriptions. Challenge studies are needed to determine pathogenicity of these strains, and to determine if current BoCV vaccines are efficacious against the BoCV clade 2 strains. C1 [Fulton, Robert W.; Herd, Heather R.; Sorensen, Nicholas J.; Confer, Anthony W.; Ritchey, Jerry W.; Burge, Lurinda J.] Oklahoma State Univ, Dept Vet Pathobiol, Stillwater, OK 74078 USA. [Ridpath, Julia F.] ARS, USDA, Natl Anim Dis Ctr, Ames, IA USA. RP Fulton, RW (reprint author), Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Room 250 McElroy Hall, Stillwater, OK 74078 USA. EM robert.fulton@okstate.edu FU Oklahoma Agricultural Experiment Station; McCasland Foundation Endowed Chair for Food Animal Research FX The research was supported by the Oklahoma Agricultural Experiment Station and funds from the McCasland Foundation Endowed Chair for Food Animal Research. NR 13 TC 2 Z9 2 U1 1 U2 11 PU SAGE PUBLICATIONS INC PI THOUSAND OAKS PA 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA SN 1040-6387 EI 1943-4936 J9 J VET DIAGN INVEST JI J. Vet. Diagn. Invest. PD JAN PY 2015 VL 27 IS 1 BP 97 EP 101 DI 10.1177/1040638714559026 PG 5 WC Veterinary Sciences SC Veterinary Sciences GA AX0PD UT WOS:000346653200014 PM 25428188 ER PT J AU Kendrick, SW Porneluzi, PA Thompson, FR Morris, DL Haslerig, JM Faaborg, J AF Kendrick, Sarah W. Porneluzi, Paul A. Thompson, Frank R., III Morris, Dana L. Haslerig, Janet M. Faaborg, John TI Stand-Level Bird Response to Experimental Forest Management in the Missouri Ozarks SO JOURNAL OF WILDLIFE MANAGEMENT LA English DT Article DE breeding birds; density; experimental study; forest management; Missouri Ozark Forest Ecosystem Project; MOFEP; spot-mapping ID NESTING SUCCESS; GROUP-SELECTION; HABITAT USE; LANDSCAPE; COMMUNITIES; POPULATIONS; CLEARCUTS; KNOWLEDGE; OVENBIRDS; SIZE AB Long-term landscape-scale experiments allow for the detection of effects of silviculture on bird abundance. Manipulative studies allow for strong inference on effects and confirmation of patterns from observational studies. We estimated bird-territory density within forest stands (2.89-62ha) for 19 years of the Missouri Ozark Forest Ecosystem Project (MOFEP), a 100-year experiment designed to study the effects of even-age and uneven-age management on wildlife. We spot-mapped territories of 15 species in 228 stands for 5 years before treatment and 14 years after treatment to assess the effects of stand-level silvicultural treatments (clearcut, select cut, thin, and no-harvest) applied within even-age, uneven-age, or no-harvest (control) management sites and year on avian territory density. We used 2 a priori contrasts to compare pre-treatment bird densities with densities during early (3-5 yr) and late (12-14 yr) post-treatment periods. The interaction of silvicultural treatment and year had significant effects on the densities of all 15 species. Densities of hooded warbler (Setophaga citrina), indigo bunting (Passerina cyanea), prairie warbler (Setophaga discolor), and yellow-breasted chat (Icteria virens) increased significantly 3-5 years post-treatment with the greatest changes in clearcuts, but densities 12-14 years post-treatment did not differ from pre-treatment densities. Densities of Acadian flycatcher (Empidonax virescens), wood thrush (Hylocichla mustelina), and especially ovenbird (Seiurus aurocapilla) had significant decreases in clearcut stands after treatment and lesser decreases in select cut or thin stands post-treatment. Densities of black-and-white warbler (Mniotilta varia), eastern wood-pewee (Contopus virens), and Kentucky warbler (Geothlypis formosa) increased in clearcut, thin, and select cut stands, but these increases were short-lived and sporadic by year after treatment. Densities of Acadian flycatcher and ovenbird remained lower in clearcut stands than no-harvest stands 13 years post-treatment. The results of this manipulative experiment were mostly consistent with our predictions of bird response to common silvicultural treatments in these forests. Managers can use these species-specific responses to silvicultural treatment to guide management decisions for target species or to balance management practices in a landscape to meet the needs of multiple species. (c) 2014 The Wildlife Society. C1 [Kendrick, Sarah W.] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. [Porneluzi, Paul A.; Morris, Dana L.] Cent Methodist Univ, Div Sci & Math, Fayette, MO 65248 USA. [Thompson, Frank R., III] Univ Missouri, USDA, US Forest Serv, Columbia, MO 65211 USA. [Haslerig, Janet M.] Missouri Dept Conservat, Jefferson City, MO 65109 USA. [Faaborg, John] Univ Missouri, Div Biol Sci, Columbia, MO 65211 USA. RP Kendrick, SW (reprint author), Univ Missouri, Div Biol Sci, 302 Anheuser Busch Nat Resources Bldg, Columbia, MO 65211 USA. EM sarahwkendrick@gmail.com FU Missouri Department of Conservation FX We thank nearly 300 MOFEP interns for their hard work in collecting these data. We are grateful to A. S. Cox and W. A. Cox for their comments on the manuscript, and J. S. Stanovick for statistical advice. Primary funding for MOFEP is provided by the Missouri Department of Conservation with additional support from the University of Missouri and the United States Forest Service Northern Research Station. NR 37 TC 9 Z9 9 U1 6 U2 48 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0022-541X EI 1937-2817 J9 J WILDLIFE MANAGE JI J. Wildl. Manage. PD JAN PY 2015 VL 79 IS 1 BP 50 EP 59 DI 10.1002/jwmg.804 PG 10 WC Ecology; Zoology SC Environmental Sciences & Ecology; Zoology GA AW9YT UT WOS:000346611800007 ER PT J AU Hosoya, T French, AD Rosenau, T AF Hosoya, Takashi French, Alfred D. Rosenau, Thomas TI Chemistry of 2,5-dihydroxyacetophenone, a Key Chromophore in Cellulosic Substrates SO MINI-REVIEWS IN ORGANIC CHEMISTRY LA English DT Article DE 2-hydroxy-acetophenones; aging; aldol reaction; bleaching; cellulose; chromophore; reaction mechanism ID ONE-POT SYNTHESIS; RESIDUAL CHROMOPHORES; HYDROQUINONE DERIVATIVES; HYDROXYLATED FLAVONES; BIOLOGICAL EVALUATION; MANGANESE-DIOXIDE; AGED CELLULOSICS; SCHIFF-BASE; DNA-BINDING; INHIBITORS AB 2,5-Dihydroxyacetophenone (DHAP) is one of the three key chromophores found in aged cellulosics. Knowledge of the general reactivity and chemistry of this compound is helpful for a better understanding of cellulose aging and yellowing as well as bleaching of cellulosic materials - processes that also have considerable economic importance. This paper reviews the reactions reported for 2,5-dihydroxyacetophenone from the viewpoints of both synthesis and general reactivity. The compound exhibits a rich chemistry due to the superposition of the hydroquinone structure and the 2-hydroxyacetophenone structure with its alpha-hydroxy ortho-quinone methide tautomer. The 1-acetyl group exhibits high reactivity attributed to resonance of the carbonyl group with the pi-electrons of the benzene ring and proton donation to the carbonyl group from the adjacent 2-hydroxy group. One of the prominent conversion paths for DHAP are aldol-type reactions that have been applied for the synthesis of a multitude of flavonoids. Other reaction pathways are nucleophilic substitution on the carbonyl group mainly with amines, electrophilic addition to the benzene ring, and oxidation into the corresponding benzoquinone. There are also quite a few reports of selective derivatization of the 5-hydroxy group of DHAP that has a higher reactivity than its 2-OH counterpart that is engaged in tight hydrogen bonding with the acetyl carbonyl moiety and thus largely blocked in esterifications and similar derivatizations. C1 [Hosoya, Takashi; Rosenau, Thomas] Univ Nat Resources & Life Sci, Dept Chem, A-1190 Vienna, Austria. [French, Alfred D.] USDA, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Rosenau, T (reprint author), Univ Nat Resources & Life Sci, Dept Chem, Muthgasse 18, A-1190 Vienna, Austria. EM thomas.rosenau@boku.ac.at FU Austrian Forschungsforderungsgesellschaft (FFG) [838422] FX We thank the Austrian Forschungsforderungsgesellschaft (FFG) for generous financial support (project 838422). NR 85 TC 0 Z9 0 U1 5 U2 17 PU BENTHAM SCIENCE PUBL LTD PI SHARJAH PA EXECUTIVE STE Y-2, PO BOX 7917, SAIF ZONE, 1200 BR SHARJAH, U ARAB EMIRATES SN 1570-193X EI 1875-6298 J9 MINI-REV ORG CHEM JI Mini-Rev. Org. Chem. PY 2015 VL 12 IS 1 BP 88 EP 95 PG 8 WC Chemistry, Organic SC Chemistry GA AX3MO UT WOS:000346843800006 ER PT J AU Novak, JM Sigua, GC Spokas, KA Busscher, WJ Cantrell, KB Watts, DW Glaz, B Hunt, PG AF Novak, J. M. Sigua, G. C. Spokas, K. A. Busscher, W. J. Cantrell, K. B. Watts, D. W. Glaz, B. Hunt, P. G. TI Plant Macro- and Micronutrient Dynamics in a Biochar-Amended Wetland Muck SO WATER AIR AND SOIL POLLUTION LA English DT Article DE Biochar; Wetlands; Soluble nutrients; Leaching ID MANAGEMENT-PRACTICES; ALTERED WETLAND; SOIL; AMENDMENT; POROSITY AB Biochar is an organic carbon (OC) and plant nutrient-rich substance that may be an ideal amendment for bolstering soil organic matter and nutrient contents. Two biochars were produced by pyrolysis at 350 degrees C from pine chips (Pinus taeda) and swine manure solids (Sus scrofa domesticus). The biochar total elemental composition was quantified using inductively coupled plasma spectrometer and their surface chemical composition examined using a combination of scanning electron microscopy (SEM) and electron dispersive spectroscopy (EDS). The biochars were mixed into triplicate pots containing Lauderhill muck (Euic, hyperthermic Lithic Haplosaprist) at 0, 2.5, 5, and 10 % (dry mass). Four simulated water infiltration events were conducted during the 124-day incubation to assess the potential alteration in the leaching potential of soluble soil nutrients. At termination, the muck's fertility characteristics were assessed, and dissolved cations were measured in water leachates. Neither biochars significantly increased the muck's OC contents. Swine manure biochar contained higher K, Mg, Na, and P concentrations, and these differences were observable in SEM and EDS as differing amounts of surface-precipitated Mg and K salts. Correspondingly, swine manure biochar at all three applications rates significantly increased Mehlich 1-s K, P, Mg, and Na concentrations. Pine chip biochar only improved the Mehlich 1-extractable K concentration but did reduce soluble P concentrations. Water leachates from swine manure biochar treated wetland soil contained significantly higher soluble P concentrations that could create water quality issue in downstream ecosystems. C1 [Novak, J. M.; Sigua, G. C.; Busscher, W. J.; Cantrell, K. B.; Watts, D. W.; Hunt, P. G.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29505 USA. [Spokas, K. A.] ARS, USDA, Soil & Water Management Unit, St Paul, MN USA. [Glaz, B.] ARS, USDA, Sugarcane Field Stn, Canal Point, FL USA. RP Novak, JM (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29505 USA. EM jeff.novak@ars.usda.gov FU National Science Foundation (NSF); Materials Research Science and Engineering Centers (MRSEC) FX We appreciate the generosity of Dr. Carl Trettin of the United States Forest Service by supplying the pine chip feedstock laboratory assistance by Sheeneka Green and Jerry- Martin II with the USDA-ARS-Coastal Plain Research Center, and field support by Velton Banks and Matthew Paige of the Florida Sugar Cane League, Inc., and Amide Tejeda and Juan Tejeda of the USDA-ARS-Sugarcane Field Station at Canal Point, FL. Parts of this work were carried out in the Characterization Facility, University of Minnesota, which receives partial support from the National Science Foundation (NSF) through the Materials Research Science and Engineering Centers (MRSEC) program. There are no conflicts of interests associated with the authors, and the research was accomplished with USDA federal funds. NR 22 TC 1 Z9 1 U1 1 U2 40 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0049-6979 EI 1573-2932 J9 WATER AIR SOIL POLL JI Water Air Soil Pollut. PD JAN PY 2015 VL 226 IS 1 AR 2228 DI 10.1007/s11270-014-2228-y PG 9 WC Environmental Sciences; Meteorology & Atmospheric Sciences; Water Resources SC Environmental Sciences & Ecology; Meteorology & Atmospheric Sciences; Water Resources GA AW9XU UT WOS:000346609300007 ER PT J AU Almeida, AM Bassols, A Bendixen, E Bhide, M Ceciliani, F Cristobal, S Eckersall, PD Hollung, K Lisacek, F Mazzucchelli, G McLaughlin, M Miller, I Nally, JE Plowman, J Renaut, J Rodrigues, P Roncada, P Staric, J Turk, R AF Almeida, A. M. Bassols, A. Bendixen, E. Bhide, M. Ceciliani, F. Cristobal, S. Eckersall, P. D. Hollung, K. Lisacek, F. Mazzucchelli, G. McLaughlin, M. Miller, I. Nally, J. E. Plowman, J. Renaut, J. Rodrigues, P. Roncada, P. Staric, J. Turk, R. TI Animal board invited review: advances in proteomics for animal and food sciences SO ANIMAL LA English DT Review DE Proteomics; farm animals; aquaculture; animal health; post-harvest ID TROUT ONCORHYNCHUS-MYKISS; DRY-CURED HAM; STAPHYLOCOCCUS-AUREUS MASTITIS; LONGISSIMUS-THORACIS MUSCLE; MULTIVARIATE DATA-ANALYSIS; GLOBULE-MEMBRANE PROTEOME; LOCAL CHICKEN BREEDS; BOVINE-MILK PROTEOME; MAREKS-DISEASE VIRUS; EGG-WHITE PROTEINS AB Animal production and health (APH) is an important sector in the world economy, representing a large proportion of the budget of all member states in the European Union and in other continents. APH is a highly competitive sector with a strong emphasis on innovation and, albeit with country to country variations, on scientific research. Proteomics (the study of all proteins present in a given tissue or fluid - i.e. the proteome) has an enormous potential when applied to APH. Nevertheless, for a variety of reasons and in contrast to disciplines such as plant sciences or human biomedicine, such potential is only now being tapped. To counter such limited usage, 6 years ago we created a consortium dedicated to the applications of Proteomics to APH, specifically in the form of a Cooperation in Science and Technology (COST) Action, termed FA1002 - Proteomics in Farm Animals: www.cost-faproteomics.org. In 4 years, the consortium quickly enlarged to a total of 31 countries in Europe, as well as Israel, Argentina, Australia and New Zealand. This article has a triple purpose. First, we aim to provide clear examples on the applications and benefits of the use of proteomics in all aspects related to APH. Second, we provide insights and possibilities on the new trends and objectives for APH proteomics applications and technologies for the years to come. Finally, we provide an overview and balance of the major activities and accomplishments of the COST Action on Farm Animal Proteomics. These include activities such as the organization of seminars, workshops and major scientific conferences, organization of summer schools, financing Short-Term Scientific Missions (STSMs) and the generation of scientific literature. Overall, the Action has attained all of the proposed objectives and has made considerable difference by putting proteomics on the global map for animal and veterinary researchers in general and by contributing significantly to reduce the East-West and North-South gaps existing in the European farm animal research. Future activities of significance in the field of scientific research, involving members of the action, as well as others, will likely be established in the future. C1 [Almeida, A. M.] CVZ Ctr Vet & Zootecnia, Inst Invest Cient Trop, P-1300477 Lisbon, Portugal. [Almeida, A. M.] CIISA Ctr Interdisciplinar Invest Sanidade Anim, P-1300477 Lisbon, Portugal. [Almeida, A. M.] UNL, Inst Tecnol Quim & Biol, P-2780157 Oeiras, Portugal. [Almeida, A. M.] IBET Inst Biol Expt & Tecnol, P-2780157 Oeiras, Portugal. [Bassols, A.] Univ Autonoma Barcelona, Fac Vet, Dept Bioquim & Biol Mol, Cerdanyola Del Valles 08193, Spain. [Bendixen, E.] Aarhus Univ, Inst Mol Biol & Genet, DK-8000 Aarhus C, Denmark. [Bhide, M.] Univ Vet Med & Pharm, Lab Biomed Microbiol & Immunol, Kosice, Slovakia. [Ceciliani, F.] Univ Milan, Dept Vet Sci & Publ Hlth, I-20133 Milan, Italy. [Cristobal, S.] Linkoping Univ, Fac Hlth Sci, Dept Clin & Expt Med, Div Cell Biol, SE-58185 Linkoping, Sweden. [Cristobal, S.] Univ Basque Country, Basque Fdn Sci, Fac Med & Dent, Ikerbasque,Dept Physiol, Leioa 48940, Bizkaia, Spain. [Eckersall, P. D.] Univ Glasgow, Inst Biodivers Anim Hlth Comparat Med, Glasgow G61 1QH, Lanark, Scotland. [Hollung, K.] Nofima AS, NO-1431 As, Norway. [Lisacek, F.] Swiss Inst Bioinformat, CH-1211 Geneva, Switzerland. [Mazzucchelli, G.] Univ Liege, Dept Chem, Mass Spectrometry Lab, GIGA Res, B-4000 Liege, Belgium. [McLaughlin, M.] Univ Glasgow, Sch Vet Med, Div Vet Biosci, Glasgow G61 1QH, Lanark, Scotland. [Miller, I.] Univ Vet Med, Inst Med Biochem, A-1210 Vienna, Austria. [Nally, J. E.] ARS, Natl Anim Dis Ctr, Bacterial Dis Livestock Res Unit, USDA, Ames, IA 50010 USA. [Plowman, J.] AgResearch, Lincoln Res Ctr, Food & Biobased Prod, Christchurch 8140, New Zealand. [Renaut, J.] Ctr Rech Publ Gabriel Lippmann, Dept Environm & Agrobiotechnol, L-4422 Belvaux, Luxembourg. [Rodrigues, P.] Univ Algarve, CCMAR Ctr Marine Sci Algarve, P-8005139 Faro, Portugal. [Roncada, P.] Univ Milan, Ist Sperimentale Italiano L Spallanzani Milano, Dept Vet Sci & Publ Hlth, I-20133 Milan, Italy. [Staric, J.] Univ Ljubljana, Clin Ruminants Ambulatory Clin, Fac Vet, Ljubljana 1000, Slovenia. [Turk, R.] Univ Zagreb, Fac Vet Med, Dept Pathophysiol, Zagreb 10000, Croatia. RP Almeida, AM (reprint author), CVZ Ctr Vet & Zootecnia, Inst Invest Cient Trop, Av Univ Tecn, P-1300477 Lisbon, Portugal. EM david.eckersall@glasgow.ac.uk RI Rodrigues, Pedro/M-3406-2013; Roncada, Paola/C-5263-2013; CIISA, FMV/D-2242-2016; Almeida, Andre/I-4369-2013; OI Rodrigues, Pedro/0000-0002-9668-1204; Roncada, Paola/0000-0002-0114-5872; Almeida, Andre/0000-0002-7810-3988; LISACEK, Frederique/0000-0002-0948-4537; Cristobal, Susana/0000-0002-3894-2218 FU European Science Foundation (Brussels, Belgium) FX All authors are members of COST Action FA1002 - Proteomics in Farm Animals consortium (www.cost-faproteomics.org) to whom funding for all networking activities described in this article is acknowledged. COST is financed by the European Science Foundation (Brussels, Belgium). Authors wish to express their gratitude to Dr Jillian Bryce, Mrs Selin Campbell (University of Glasgow, UK); Mr Christophe Peeters and Dr Ioanna Stravridou (COST office, Brussels, Belgium) for their decisive contribution in the administration of FA1002. NR 160 TC 23 Z9 23 U1 8 U2 73 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD JAN PY 2015 VL 9 IS 1 BP 1 EP 17 DI 10.1017/S1751731114002602 PG 17 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AW9NS UT WOS:000346585000001 PM 25359324 ER PT J AU Kim, DH McLeod, KR Koontz, AF Foote, AP Klotz, JL Harmon, DL AF Kim, D. H. McLeod, K. R. Koontz, A. F. Foote, A. P. Klotz, J. L. Harmon, D. L. TI Effect of intake on fasting heat production, respiratory quotient and plasma metabolites measured using the washed rumen technique SO ANIMAL LA English DT Article DE intake; fasting heat production; respiratory quotient; ruminant ID MAINTENANCE ENERGY-REQUIREMENTS; FOOD-INTAKE; STEERS; TEMPERATURE; EXPENDITURE; RUMINANTS; HEIFERS; KETOSIS; STRESS; CATTLE AB The objective was to investigate the effect of intake before fasting on concentrations of metabolites and hormones, respiratory quotient (RQ) and fasting heat production (HP) using the washed rumen technique and to compare these values with those from the fed state. Six Holstein steers (360 +/- 22 kg) were maintained at 21 degrees C and fed three different energy intakes within a replicated 3 x 3 Latin square design with 21-day periods. Steers were fed alfalfa cubes to provide 1.0, 1.5 and 2.0 x NEm during 19 days of each experimental period. Steers were placed in individual metabolism stalls fitted with indirect calorimetry head-boxes on day 20 of each experimental period (FED steers) and fed their normal meal. On day 21 of each period the reticulorumen was emptied, washed and refilled with ruminal buffer (NaCl = 96; NaHCO3 = 24; KHCO3 = 30; K2HPO4 = 2; CaCl2 = 1.5; MgCl2 = 1.5 mmol/kg of buffer) aerated with 75% N-2 and 25% CO2 before introduction to the rumen (steers were not fed; WASHED steers). Each gas exchange was measured over 24 h. HP for 1.0, 1.5 and 2.0 x NEm were 479, 597 and 714 kJ/day kg(0.75) (s.e.m. = 16), respectively. The plateau RQ was 0.756, 0.824 and 0.860 for the 1.0, 1.5 and 2.0 x NEm intakes for the FED steers, respectively. After rumen washing, fasting HP was 331, 359 and 400 kJ/day kg(0.75) (s.e.m. = 13) for 1.0, 1.5, and 2.0 x NEm intakes before fasting, respectively. The RQ for WASHED rumen steers was 0.717, 0.710 and 0.719, respectively. Cortisol and beta-hydroxybutyrate concentrations in WASHED rumen steers did not exceed threshold levels for severe energy deficit and stress as can be induced from prolonged fasting. This study demonstrates that a fasting state can be emulated using the washed rumen technique, minimizing the time required as opposed to traditional fasting methodologies, without causing a severe energy deficit C1 [Kim, D. H.; McLeod, K. R.; Koontz, A. F.; Foote, A. P.; Harmon, D. L.] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA. [Klotz, J. L.] Univ Kentucky, USDA ARS, Forage Anim Prod Res Unit, Lexington, KY 40546 USA. RP Kim, DH (reprint author), Rural Dev Adm, Natl Inst Anim Sci, Suwon 441706, South Korea. EM dharmon@uky.edu FU federal funds from the U.S. Department of Agriculture, Agricultural Research Service; Kentucky Agricultural Experiment Station [13-07-108] FX Support for this research was provided in part by federal funds from the U.S. Department of Agriculture, Agricultural Research Service and by the Kentucky Agricultural Experiment Station and Publication No. 13-07-108. The authors express gratitude to Susan Hayes of the University of Kentucky for assistance with plasma radioimmunoassay. NR 30 TC 0 Z9 0 U1 2 U2 14 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 1751-7311 EI 1751-732X J9 ANIMAL JI Animal PD JAN PY 2015 VL 9 IS 1 BP 58 EP 66 DI 10.1017/S1751731114002183 PG 9 WC Agriculture, Dairy & Animal Science; Veterinary Sciences SC Agriculture; Veterinary Sciences GA AW9NS UT WOS:000346585000007 PM 25166735 ER PT J AU Diaz, R Manrique, V Munyaneza, JE Sengoda, VG Adkins, S Hendricks, K Roberts, PD Overholt, WA AF Diaz, Rodrigo Manrique, Veronica Munyaneza, Joseph E. Sengoda, Venkatesan G. Adkins, Scott Hendricks, Katherine Roberts, Pamela D. Overholt, William A. TI Host specificity testing and examination for plant pathogens reveals that the gall-inducing psyllid Calophya latiforceps is safe to release for biological control of Brazilian peppertree SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE Anacardiaceae; Calophyidae; Candidatus Liberibacter; Hemiptera; invasive plant; leaf gall; plant virus; Schinus terebinthifolia ID SCHINUS-TEREBINTHIFOLIUS ANACARDIACEAE; CANDIDATUS LIBERIBACTER SOLANACEARUM; REAL-TIME PCR; HEMIPTERA PSYLLOIDEA; LICE HEMIPTERA; FLORIDA; DISEASES; POTATO; HERBIVORES; INDUCTION AB Brazilian peppertree, Schinus terebinthifolia Raddi (Anacardiaceae), is one of the worst upland exotic weeds in Florida, USA. Foreign exploration for natural enemies led to the discovery of a pit-galling psyllid, Calophya latiforceps Burckhardt (Hemiptera: Calophyidae), in the state of Bahia, Brazil, in 2010. Crawlers of C.latiforceps stimulate the formation of galls on the leaves of S.terebinthifolia resulting in leaf discoloration and in some cases leaf abscission. To determine whether C.latiforceps is a safe candidate for biological control of S.terebinthifolia, host specificity and the presence of selected plant pathogens were examined. Adult oviposition, gall formation, and adult survival of C.latiforceps were examined on 89 plant species under no-choice and choice conditions. We found that C.latiforceps laid eggs on plants in seven families; however, crawlers stimulated gall formation and completed development to adult only on S.terebinthifolia. All crawlers on non-target plants died, likely due to starvation caused either by the absence of a feeding stimulus or by a hypersensitive plant response. Under no-choice conditions, 10% of adults lived for 19days on the target weed, but adult survival was reduced to <3days on non-target plants. Choice testing revealed that females preferred to oviposit on S.terebinthifolia compared to non-target plants. Molecular methods and indicator host inoculations did not detect the presence of Candidatus Liberibacter solanacearum', Ca.L. asiaticus', Ca.L. americanus', Ca.L. africanus', or plant viruses in adult C.latiforceps. We conclude that releasing C.latiforceps in the USA will have extremely low risk to non-target plants, and provides another tool for the management of S.terebinthifolia. C1 [Diaz, Rodrigo; Manrique, Veronica; Overholt, William A.] Univ Florida, Biol Control Res & Containment Lab, Ft Pierce, FL 34945 USA. [Munyaneza, Joseph E.; Sengoda, Venkatesan G.] USDA ARS, Yakima Agr Res Lab, Wapato, WA 98951 USA. [Adkins, Scott] USDA ARS, United States Hort Res Lab, Ft Pierce, FL 34945 USA. [Hendricks, Katherine; Roberts, Pamela D.] Univ Florida, Southwest Florida Res & Educ Ctr, Immokalee, FL 34142 USA. RP Diaz, R (reprint author), Univ Florida, Biol Control Res & Containment Lab, 2199 S Rock Rd, Ft Pierce, FL 34945 USA. EM rrdg@ufl.edu FU Florida Fish and Wildlife Commission; Florida Department of Agriculture and Consumer Services FX Patricia Prade, Elizabeth Calise, Emily Westermeier, and Amy Dubois from the Weed Biological Control Program at BCRCL provided assistance in maintenance of insect and plant colonies and the data collection. Carrie Vanderspool at USDA-ARS in Fort Pierce provided excellent technical assistance in plant- virus testing. Special thanks to Amanda, Carmen, and Reinaldo Rios for logistical support during the collection of C. latiforceps in Bahia, Brazil. We thank Drs. James P. Cuda and Gregory Wheeler for providing several test plants. Dr. Susan Halbert fromthe Florida Department of Agriculture and Consumer Services confirmed the identification of C. latiforceps on several occasions. This project was supported in part by the Florida Fish and Wildlife Commission, and the Florida Department of Agriculture and Consumer Services. The manuscript was improved based on comments by three anonymous reviewers. NR 64 TC 0 Z9 1 U1 4 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JAN PY 2015 VL 154 IS 1 BP 1 EP 14 DI 10.1111/eea.12249 PG 14 WC Entomology SC Entomology GA AW8CH UT WOS:000346487700001 ER PT J AU Hagler, JR Machtley, SA Blackmer, F AF Hagler, James R. Machtley, Scott A. Blackmer, Felisa TI A potential contamination error associated with insect protein mark-capture data SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE insect marking; ELISA; Hippodamia convergens; sweep net sampling; Coleoptera; Coccinellidae; alfalfa field; Medicago sativa; Fabaceae; false-positive mark ID IMMUNOLOGICAL APPROACH; ORGANIC STRAWBERRIES; PREDATOR ASSEMBLAGE; MOLECULAR ANALYSIS; FORAGING BEHAVIOR; DISPERSAL; IMMUNOMARKING; MOVEMENT; SPP.; ARGENTIFOLII AB Various types of protein-spray solutions have proven effective for externally tagging arthropods for mark-release-recapture and mark-capture type dispersal research. However, there is concern that certain standardized arthropod collection methods, such as sweep netting, might lead to high incidences of protein transfer from field-marked to unmarked arthropods during sample collection and sample handling. Native arthropods were collected in sweep nets from a field of alfalfa, Medicago sativa L. (Fabaceae). The nets also contained 10 egg white-, 10 bovine milk-, 10 soy milk-, and 10 water (control)-marked Hippodamia convergens Guerin-Meneville (Coleoptera: Coccinellidae) that were visually distinguishable by a yellow, white, green, and blue dot, respectively. The plant debris and arthropods from each sweep net collection were then placed into either a paper or a plastic bag andfrozen for storage. The contents of each sweep net sample were thawed and the color-coded H.convergens and field-collected arthropods were examined for the presence of each protein by an egg white (albumin), bovine milk (casein), and soy milk (soy trypsin) enzyme-linked immunosorbent assay (ELISA). Data revealed that only 0.67, 0.81, and 0% of the field-collected unmarked arthropods acquired an egg white, bovine milk, and soy milk mark, respectively. ELISA results also showed that all the egg white-marked H.convergens retained their mark, but 22.1% of the bovine milk-marked and 5.1% of the soy milk-marked H.convergens (color-coded beetles) lost their mark during the collection and sample handling processes. C1 [Hagler, James R.; Machtley, Scott A.; Blackmer, Felisa] USDA ARS, Arid Land Agr Res Ctr, Maricopa, AZ 85138 USA. RP Hagler, JR (reprint author), USDA ARS, Arid Land Agr Res Ctr, 21881 North Cardon Lane, Maricopa, AZ 85138 USA. EM james.hagler@ars.usda.gov FU USDA CRIS [5347-22620-021-00D]; USDA National Institute of Food and Agriculture [2011-67009-30141] FX We thank Sara Slosky, Juan Sandoval, Dana Zack, and Gabe Zilnik for their technical assistance. This project was supported by USDA CRIS 5347-22620-021-00D and, in part, by Agriculture and Food Research Initiative Competitive Grant no. 2011-67009-30141 from the USDA National Institute of Food and Agriculture. Mention of trade names or commercial products in this publication is solely for providing specific information and does not imply recommendation or endorsement by the USDA. NR 33 TC 1 Z9 1 U1 5 U2 29 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JAN PY 2015 VL 154 IS 1 BP 28 EP 34 DI 10.1111/eea.12252 PG 7 WC Entomology SC Entomology GA AW8CH UT WOS:000346487700004 ER PT J AU Kuenen, LPS Hicks, MN AF Kuenen, L. P. S. Hicks, Matt N. TI Gas chromatography column as an ambient-temperature volatile trap SO ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA LA English DT Article DE open tube volatile traps; pheromone collection; volatile ratios; capillaries; adsorption; Oriental fruit moth; method comparison; Grapholita molesta; Tortricidae; Lepidoptera ID ORIENTAL FRUIT MOTH; SEX-PHEROMONE COMPONENTS; TRICHOPLUSIA-NI; RELEASE RATES; RETENTION GAP; LEPIDOPTERA; COLLECTION; TORTRICIDAE; IDENTIFICATION; FORMULATIONS AB Open-tube volatile traps have largely been shunned in favor of solid adsorbent containing traps for the collection of volatile pheromones and attractants. Solid adsorbents require large solvent rinses and glass capillaries can be difficult to maneuver for the collection of volatiles from small or hard-to-reach odor sources. A gas chromatograph (GC) column (DB-1), an open-tube glass capillary, and a SuperQ((R))-containing capillary were compared for their collection efficiencies from rubber septa and live calling insects. All three traps captured similar ratios of test compounds from septa at airflows >10ml per min. Eluting analytes from a packed adsorbent, SuperQ, required at least 30x more solvent than was required to collect all the pheromone from the open-tube glass capillaries, and the GC column enjoyed an additional three-fold reduced solvent volume compared to the glass capillary. Thus, analytes could be eluted from the GC-column trap and directly analyzed on GC without solvent evaporation. We placed glass wool plugs' in both GC columns and glass capillaries and found no volatiles in these plugs, indicating that breakthrough did not occur during 1-h collections at 25ml per min. We demonstrate here that at ambient laboratory temperatures, a DB-1 GC column effectively collects Oriental fruit moth sex pheromone volatiles from a rubber septum and live pheromone-releasing moths. Release ratios of pheromone from rubber septa are consistent with earlier reports from static air systems, whereas the release ratio of the (Z)-8-dodecenyl alcohol (Z8-12:OH) from female Grapholita molesta Busck (Lepidoptera: Tortricidae) differed from published results and is likely due to different collection methods or moth-strain origin. C1 [Kuenen, L. P. S.; Hicks, Matt N.] ARS, USDA, SJV ASC, Parlier, CA 93648 USA. [Hicks, Matt N.] Calif State Univ Fresno, Dept Biol, Fresno, CA 93740 USA. RP Kuenen, LPS (reprint author), ARS, USDA, SJV ASC, 9611 S Riverbend Ave, Parlier, CA 93648 USA. EM bas.kuenen@ars.usda.gov FU California Pistachio Research Board FX This study was funded in part by the California Pistachio Research Board. Mention of trade names or commercial products in this article is solely for providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. NR 30 TC 2 Z9 2 U1 3 U2 22 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0013-8703 EI 1570-7458 J9 ENTOMOL EXP APPL JI Entomol. Exp. Appl. PD JAN PY 2015 VL 154 IS 1 BP 35 EP 44 DI 10.1111/eea.12253 PG 10 WC Entomology SC Entomology GA AW8CH UT WOS:000346487700005 ER PT J AU Benjamin, LA Jay-Russell, MT Atwill, ER Cooley, MB Carychao, D Larsen, RE Mandrell, RE AF Benjamin, L. A. Jay-Russell, M. T. Atwill, E. R. Cooley, M. B. Carychao, D. Larsen, R. E. Mandrell, R. E. TI Risk factors for Escherichia coli O157 on beef cattle ranches located near a major produce production region SO EPIDEMIOLOGY AND INFECTION LA English DT Article DE Cattle; Escherichia coli O157; multi-locus variable number tandem repeat analysis; risk factors ID CENTRAL CALIFORNIA COAST; CALVES; PREVALENCE; RANGE; INFECTION; OUTBREAK; WATER; SURVIVAL; SPINACH; FARMS AB Our goal was to identify climate variables and management practices associated with the presence of E. coli O157 in rangeland cow-calf operations located in a major leafy green production region in the California Central Coast. E. coli O157 was present in 26% (68/2654) of faecal, 15% (3/204) of water and 11% (1/93) of sediment samples collected on eight ranches over 2.5 years. Five (625%) ranches were positive at least once during the study. The odds of detecting E. coli O157 in faecal samples was higher during periods of higher maximum soil temperature, higher maximum relative humidity, and larger herd sizes, but decreased as wind speed increased. Molecular subtyping of isolates from cattle faeces and streams/sediments suggested minimal movement of strains between ranches. The findings suggest that E. coli O157 prevalence is relatively low on cow-calf ranches in this region, spatially constrained, but may vary by weather conditions and herd size. C1 [Benjamin, L. A.; Jay-Russell, M. T.; Atwill, E. R.] Univ Calif Davis, Western Ctr Food Safety, Davis, CA 95618 USA. [Cooley, M. B.; Carychao, D.; Mandrell, R. E.] ARS, Prod Safety & Microbiol Res Unit, USDA, Western Reg Res Ctr, Albany, CA USA. [Larsen, R. E.] Univ Calif Cooperat Extens, Templeton, CA USA. RP Jay-Russell, MT (reprint author), Univ Calif Davis, Western Ctr Food Safety, 1477 Drew Ave,Ste 101, Davis, CA 95618 USA. EM mjay@ucdavis.edu FU U.S. Food and Drug Administration Project [U01-003-572]; National Research Initiative Competitive Grant from the U.S. Department of Agriculture National Institute of Food and Agriculture [2006-55212-16927, 2007-35212-18239]; U.S. Department of Agriculture, Agricultural Research Service, CRIS project [5325-42000-044-00D]; Center for Food Safety and Applied Nutrition FX This research was funded by the U.S. Food and Drug Administration Project No. U01-003-572; by National Research Initiative Competitive Grant nos. 2006-55212-16927 and 2007-35212-18239 from the U.S. Department of Agriculture National Institute of Food and Agriculture; and the U.S. Department of Agriculture, Agricultural Research Service, CRIS project number 5325-42000-044-00D. This project was also supported in part by an appointment to the Research Participation at the Center for Food Safety and Applied Nutrition administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy and the U.S. Food and Drug Administration. NR 40 TC 2 Z9 2 U1 3 U2 22 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0950-2688 EI 1469-4409 J9 EPIDEMIOL INFECT JI Epidemiol. Infect. PD JAN PY 2015 VL 143 IS 1 BP 81 EP 93 DI 10.1017/S0950268814000521 PG 13 WC Public, Environmental & Occupational Health; Infectious Diseases SC Public, Environmental & Occupational Health; Infectious Diseases GA AW3KZ UT WOS:000346186800009 PM 24650854 ER PT J AU Arndt, C Powell, JM Aguerre, MJ Wattiaux, MA AF Arndt, C. Powell, J. M. Aguerre, M. J. Wattiaux, M. A. TI Performance, digestion, nitrogen balance, and emission of manure ammonia, enteric methane, and carbon dioxide in lactating cows fed diets with varying alfalfa silage-to-corn silage ratios SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE greenhouse gas; rumen fermentation; nitrogen utilization; forage; soybean ID MILK UREA NITROGEN; DAIRY-COWS; CONCENTRATE RATIO; BEEF HEIFERS; PROTEIN; DIGESTIBILITY; FORAGES; STARCH; FERMENTABILITY; MANIPULATION AB Two trials were conducted simultaneously to study the effects of varying alfalfa silage (AS) to corn silage (CS) ratio in diets formulated to avoid excess protein or starch on lactating dairy cow performance, digestibility, ruminal parameters, N balance, manure production and composition, and gaseous emissions [carbon dioxide (CO2), methane (CH4), and ammonia-N (NH3-N)]. In trial 1 all measurements, except gas emissions, were conducted on 8 rumen-cannulated cows in replicated 4 x 4 Latin squares. In trial 2, performance and emissions were measured on 16 cows randomly assigned to 1 of 4 air-flow controlled chambers in a 4 x 4 Latin square. Dietary treatments were fed as total mixed rations with forage-to-concentrate ratio of 55:45 [dietary dry matter (DM) basis] and AS:CS ratios of 20:80, 40:60, 60:40, and 80:20 (forage DM basis). Measurements were conducted the last 3 d of each 21-d period. Treatments did not affect DM intake, DM digestibility, and milk/DM intake. However, responses were quadratic for fat-andprotein-corrected milk, fat, and protein production, which reached predicted maxima for AS:CS ratio of 50:50, 49:51, and 34:66, respectively. Nitrogen use efficiency (milk N/N intake) decreased from 31 to 24 g/100 g as AS:CS ratio increased from 20:80 to 80:20. Treatments did not alter NH3-N/milk-N but tended to have a quadratic effect on daily NH3-N emission. Treatments had a quadratic effect on daily CH4 emission, which was high compared with current literature; they influenced CH4 emission per unit of neutral detergent fiber (NDF) intake and tended to influence CO2/NDF intake. Ruminal acetate-to-propionate ratio and total-tract NDF digestibility increased linearly with increasing AS:CS ratio. In addition, as AS:CS ratio increased from 20:80 to 80:20, NDF digested increased linearly from 2.16 to 3.24 kg/d, but CH4/digested NDF decreased linearly from 270 to 190 g/kg. These 2 counterbalancing effects likely contributed to the observed quadratic response in daily CH4 emission, which may have been influenced also by increasing starch with increasing CS in the diet as reflected by the increased ruminal propionate molar proportion. Overall, production performances were greatest for the intermediate AS:CS ratios (40:60 and 60:40), but daily excretion of urine, manure, fecal N, urinary urea N, and urinary N decreased with increasing proportion of CS in the diet, whereas daily CH4 emision was reduced for the 2 extreme AS:CS ratios (20:80 and 80:20). However, the proportion of AS and CS in the diet did not affect CH4/fat-and-protein corrected milk. C1 [Arndt, C.; Aguerre, M. J.; Wattiaux, M. A.] Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. [Powell, J. M.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. RP Wattiaux, MA (reprint author), Univ Wisconsin, Dept Dairy Sci, Madison, WI 53706 USA. EM wattiaux@wisc.edu FU USDA-Agricultural Research Service [58-3655-7-627]; USDA-Hatch Multi-State Research Formula fund [NE-1044, WIS01547] FX This research was supported in part by the USDA-Agricultural Research Service specific cooperative agreement #58-3655-7-627 and by USDA-Hatch Multi-State Research Formula fund NE-1044 award number WIS01547. The authors thank Robbin Smith for her assistance in chamber maintenance and conduct of these experiments; Nancy Betzold and her barn crew for feeding and animal care at the US Dairy Forage Center Research Farm (Prairie du Sac, WI); and Eric Ronk and other University of Wisconsin Madison students for helping during sample collection and processing. NR 50 TC 12 Z9 14 U1 7 U2 73 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JAN PY 2015 VL 98 IS 1 BP 418 EP 430 DI 10.3168/jds.2014-8298 PG 13 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AX0DC UT WOS:000346622600045 PM 25465537 ER PT J AU Brito, AF Petit, HV Pereira, ABD Soder, KJ Ross, S AF Brito, A. F. Petit, H. V. Pereira, A. B. D. Soder, K. J. Ross, S. TI Interactions of corn meal or molasses with a soybean-sunflower meal mix or flaxseed meal on production, milk fatty acid composition, and nutrient utilization in dairy cows fed grass hay-based diets SO JOURNAL OF DAIRY SCIENCE LA English DT Article DE grass hay; flaxseed meal; lactating dairy cow; liquid molasses ID MAMMALIAN LIGNANS ENTEROLACTONE; IN-VITRO METABOLISM; MICROBIAL PROTEIN; LINOLEIC-ACID; SERUM CONCENTRATIONS; TRACT DIGESTIBILITY; RUMINAL METABOLISM; PURINE DERIVATIVES; PHYTO-ESTROGENS; HOLSTEIN COWS AB We investigated the interactions of corn meal or molasses [nonstructural carbohydrate (NSC) supplements] with a soybean-sunflower meal mix or flaxseed meal [rumen-degradable protein (RDP) supplements] on animal production, milk fatty acids profile, and nutrient utilization in dairy cows fed grass hay diets. Eight multiparous and 8 primiparous Jersey cows averaging 135 +/- 49 d in milk and 386 +/- 61 kg of body weight in the beginning of the study were randomly assigned to 4 replicated 4 x 4 Latin squares with a 2 x 2 factorial arrangement of treatments. Each period lasted 19 d with 14 d for diet adaptation and 5 d for data and sample collection. Cows were fed diets composed of mixedmostly grass hay plus 1 of the following 4 concentrate blends: (1) corn meal plus a protein mix containing soybean meal and sunflower meal; (2) corn meal plus flaxseed meal; (3) liquid molasses plus a protein mix containing soybean meal and sunflower meal; or (4) liquid molasses plus flaxseed meal. Data were analyzed for main effects of NSC and RDP supplements, and the NSC x RDP supplement interactions. Significant NSC x RDP supplement interactions were observed for milk urea N, milk N efficiency, and the sums of milk saturated, monounsaturated, and polyunsaturated fatty acids. No effect of NSC supplements was observed for nutrient intake and milk yield. However, 4% fat-corrected milk (-0.70 kg/d) and energy-corrected milk (-0.60 kg/d) were significantly reduced in cows fed liquid molasses due to a trend to decreased concentration of milk fat (-0.17%). Diets with liquid molasses resulted in increased (+35%) concentration and yield of milk enterolactone, indicating that this mammalian lignan can be modulated by supplements with different NSC profiles. Overall, NSC and RDP supplements pro- foundly changed the milk fatty acid profile, likely because of differences in fatty acids intake, Delta(9)-desaturase indices, and ruminal biohydrogenation pathways. Feeding liquid molasses significantly reduced plasma urea N (-1.2 mg/dL), urinary N excretion (-20 g/d), and N digestibility (-3.2 percentage units) Flaxseed meal significantly reduced yields of milk (-1.3 kg/d), milk fat (-90 g/d), and milk lactose (-60 g/d), but significantly increased the concentration and yield of milk enterolactone. Further research is needed to elucidate the negative responses of flaxseed meal on yields of milk and milk components. C1 [Brito, A. F.; Pereira, A. B. D.; Ross, S.] Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. [Petit, H. V.] Agr & Agri Food Canada, Dairy & Swine Res & Dev Ctr, Sherbrooke, PQ J1M 0C8, Canada. [Soder, K. J.] USDA ARS, Pasture Syst & Watershed Management Res Unit, University Pk, PA 16802 USA. RP Brito, AF (reprint author), Univ New Hampshire, Dept Biol Sci, Durham, NH 03824 USA. EM andre.brito@unh.edu OI Pereira, Andre/0000-0002-4788-0338 FU New Hampshire Agricultural Experiment Station; USDA Multistate Project [NC-1042] FX The authors thank the New Hampshire Agricultural Experiment Station for financial support. Gratitude is extended to Nancy Whitehouse (University of New Hampshire) and Liette Veilleux (Agriculture and Agri-Food Canada/Dairy and Swine Research and Development Centre) for sample collection and technical assistance. We also thank the University of New Hampshire Burley-Demeritt Organic Dairy Research Farm former manager Trent Schrieffer and his staff for animal care and overall researeh support. The present research was a component of the USDA Multistate Project NC-1042. NR 62 TC 3 Z9 3 U1 0 U2 29 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 0022-0302 EI 1525-3198 J9 J DAIRY SCI JI J. Dairy Sci. PD JAN PY 2015 VL 98 IS 1 BP 443 EP 457 DI 10.3168/jds.2014-8353 PG 15 WC Agriculture, Dairy & Animal Science; Food Science & Technology SC Agriculture; Food Science & Technology GA AX0DC UT WOS:000346622600047 PM 25465544 ER PT J AU Davies, VF Kupek, E de Assis, MA Engel, R da Costa, FF Pietro, PF Natal, S Thompson, D Baranowski, T AF Davies, V. F. Kupek, E. de Assis, M. A. Engel, R. da Costa, F. F. Di Pietro, P. F. Natal, S. Thompson, D. Baranowski, T. TI Qualitative analysis of the contributions of nutritionists to the development of an online instrument for monitoring the food intake of schoolchildren SO JOURNAL OF HUMAN NUTRITION AND DIETETICS LA English DT Article DE focus group discussions; nutritionists; questionnaire; web-based ID DIETARY-INTAKE; CHILDREN; TECHNOLOGIES; OBESITY; QUESTIONNAIRE; ADOLESCENTS; VALIDITY; POLICIES; TOOL AB Background The Consumo Alimentar e Atividade Fisica de Escolares (CAAFE) questionnaire is an online research tool that has been developed to enable the self-report of physical activity and diet by Brazilian schoolchildren aged 7-10years. Formative research was conducted with nutritionists during the development of the web-based questionnaire. The suggestions and insights obtained were used to design a tool to monitor schoolchildren's food consumption based on the concept of healthy and unhealthy food indicators. The present study aimed to report the focus group discussions conducted with nutritionists concerning the CAAFE questionnaire. MethodsFocus group discussions were conducted using a semi-structured questionnaire, and these were then analysed thematically. ResultsTwenty-four nutritionists participated (four focus groups; average per group: six people); the majority (n=22) had experience with 7-10-year-old children. Four themes emerged: (i) healthy and unhealthy food indicators; (ii) suggestions for the online instrument; (iii) potential applications; and (iv) challenges for its construction. ConclusionsComments made by nutritionists enabled the construction of an instrument that is able to answer questions related to food consumption in schools and at home. C1 [Davies, V. F.; Kupek, E.; Natal, S.] Fed Univ Santa, Grad Program Publ Hlth, Ctr Hlth Sci, Florianopolis, SC, Brazil. [de Assis, M. A.; Engel, R.; Di Pietro, P. F.] Univ Fed Santa Catarina, Ctr Hlth Sci, Grad Program Nutr, BR-88040370 Florianopolis, SC, Brazil. [de Assis, M. A.; da Costa, F. F.] Univ Fed Santa Catarina, Sports Ctr, Grad Program Phys Educ, BR-88040370 Florianopolis, SC, Brazil. [Thompson, D.; Baranowski, T.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX USA. RP Davies, VF (reprint author), Univ Fed Santa Catarina, Ctr Hlth Sci, Grad Program Publ Hlth, Campus Reitor Joao David Ferreira Lima,Rua Delfin, BR-88040370 Florianopolis, SC, Brazil. EM va.davies@hotmail.co.uk RI de Assis, Maria Alice/B-8125-2009; Assis, Maria Alice/L-8489-2015; OI Assis, Maria Alice/0000-0002-5383-3714; Baranowski, Tom/0000-0002-0653-2222 FU Brazilian Ministry of Health (Departamento de Ciencia, Tecnologia e Insumos Estrategicos - DECIT); Brazilian Ministry of Education (Coordenacao de Aperfeicoamento de Pessoal de NivelSuperior-CAPES); Brazilian Ministry of Science, Technology and Innovation (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq); CAPES - Brazilian Federal Agency for Support and Evaluation of Graduate Education FX This research was funded by a grant from the Brazilian Ministry of Health (Departamento de Ciencia, Tecnologia e Insumos Estrategicos - DECIT), the Brazilian Ministry of Education (Coordenacao de Aperfeicoamento de Pessoal de NivelSuperior-CAPES) and the Brazilian Ministry of Science, Technology and Innovation (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). VFD was supported by the CAPES - Brazilian Federal Agency for Support and Evaluation of Graduate Education. NR 33 TC 1 Z9 2 U1 1 U2 4 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0952-3871 EI 1365-277X J9 J HUM NUTR DIET JI J. Hum. Nutr. Diet. PD JAN PY 2015 VL 28 SU 1 SI SI BP 65 EP 72 DI 10.1111/jhn.12209 PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AW5ZI UT WOS:000346349000007 PM 24480047 ER PT J AU Davies, VF Kupek, E de Assis, MA Natal, S Pietro, PF Baranowski, T AF Davies, V. F. Kupek, E. de Assis, M. A. Natal, S. Di Pietro, P. F. Baranowski, T. TI Validation of a web-based questionnaire to assess the dietary intake of Brazilian children aged 7-10 years SO JOURNAL OF HUMAN NUTRITION AND DIETETICS LA English DT Article DE intrusions; matches; omissions; online; questionnaire ID ADOLESCENTS; TECHNOLOGIES; PREVALENCE; SOFTWARE; OBESITY; FRUIT AB BackgroundThe Food Intake and Physical Activity of School Children (CAAFE) comprises an online questionnaire to self-report diet and physical activity of Brazilian schoolchildren. BackgroundThe present study aimed to assess the validity (matches, omissions and intrusions) and moderating factors of the CAAFE. MethodsDirect observation was made of foods consumed (five public schools) and child self-reporting on the CAAFE. Additional data included school grade, gender, body mass index, completion of food diary, socioeconomic status and access to computer. Data were analysed using regression. ResultsIn total, 602 children participated in the study [mean (SD) age 9.5(1.24)years; 53.6% boys]. On average, there were 43% matches, 29% intrusions and 28% omissions. Matches doubled in third grade compared to the second grade (P=0.004); matches almost tripled for afternoon snack compared to morning snack (P<0.001); and matches were 69% higher for children with access to a computer at home (P<0.01). Intrusions decreased by almost one-half in fifth compared to fourth grades (P=0.004). Omissions declined significantly in the third and fourth grades but increased in the fifth grade. Omissions were 47% lower for children in the highest income and lower among children who completed the food diary. No differences were found for gender or body mass index. ConclusionsChildren older than 8years old, who owned a computer and completed a food diary, performed better in the CAAFE. A high incidence of disagreement was found in relation to the schools and the type of meal. Overall matches (43%), intrusions (29%) and omissions (28%) indicate that further studies are required to improve the validity of the CAAFE. C1 [Davies, V. F.; Kupek, E.; Natal, S.] Univ Fed Santa Catarina, Grad Program Publ Hlth, Ctr Hlth Sci, BR-88040370 Florianopolis, SC, Brazil. [de Assis, M. A.; Di Pietro, P. F.] Univ Fed Santa Catarina, Grad Program Nutr, Ctr Hlth Sci, BR-88040370 Florianopolis, SC, Brazil. [Baranowski, T.] USDA ARS, Childrens Nutr Res Ctr, Dept Pediat, Baylor Coll Med, Houston, TX USA. RP Davies, VF (reprint author), Univ Fed Santa Catarina, Grad Program Publ Hlth, Ctr Hlth Sci, Campus Reitor Joao David Ferreira Lima,Rua Delfin, BR-88040370 Florianopolis, SC, Brazil. EM va.davies@hotmail.co.uk RI de Assis, Maria Alice/B-8125-2009; Assis, Maria Alice/L-8489-2015; OI Assis, Maria Alice/0000-0002-5383-3714; Baranowski, Tom/0000-0002-0653-2222 FU Brazilian Ministry of Health (Departamento de Ciencia, Tecnologia e Insumos Estrategicos - DECIT); Brazilian Ministry of Education (Coordenacao de Aperfeicoamento de Pessoal de NivelSuperior-CAPES); Brazilian Ministry of Science, Technology and Innovation (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)); CAPES - Brazilian Federal Agency for Support and Evaluation of Graduate Education FX This research was funded by a grant from the Brazilian Ministry of Health (Departamento de Ciencia, Tecnologia e Insumos Estrategicos - DECIT), the Brazilian Ministry of Education (Coordenacao de Aperfeicoamento de Pessoal de NivelSuperior-CAPES) and the Brazilian Ministry of Science, Technology and Innovation (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq). VFD was supported by the CAPES - Brazilian Federal Agency for Support and Evaluation of Graduate Education. NR 35 TC 5 Z9 6 U1 3 U2 7 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0952-3871 EI 1365-277X J9 J HUM NUTR DIET JI J. Hum. Nutr. Diet. PD JAN PY 2015 VL 28 SU 1 SI SI BP 93 EP 102 DI 10.1111/jhn.12262 PG 10 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AW5ZI UT WOS:000346349000010 PM 25139011 ER PT J AU Callicott, KA Cotty, PJ AF Callicott, K. A. Cotty, P. J. TI Method formonitoring deletions in the aflatoxin biosynthesis gene cluster of Aspergillus flavus with multiplex PCR SO LETTERS IN APPLIED MICROBIOLOGY LA English DT Article DE aflatoxin; Aspergillus flavus; biocontrol; cyclopiazonic acid; multiplex PCR; subtelomere stability ID BIOLOGICAL-CONTROL; COST-EFFECTIVENESS; CONTAMINATION; COTTONSEED; SEQUENCE; STRAINS; ORYZAE; HEALTH AB The report presents a rapid, inexpensive and simple method for monitoring indels with influence on aflatoxin biosynthesis within Aspergillus flavus populations. PCR primers were developed for 32 markers spaced approximately every 5kb from 20kb proximal to the aflatoxin biosynthesis gene cluster to the telomere repeat. This region includes gene clusters required for biosynthesis of aflatoxins and cyclopiazonic acid; the resulting data were named cluster amplification patterns (CAPs). CAP markers are amplified in four multiplex PCRs, greatly reducing the cost and time to monitor indels within this region across populations. The method also provides a practical tool for characterizing intraspecific variability in A.flavus not captured with other methods. Significance and Impact of the StudyAflatoxins, potent naturally-occurring carcinogens, cause significant agricultural problems. The most effective method for preventing contamination of crops with aflatoxins is through use of atoxigenic strains of Aspergillus flavus to alter the population structure of this species and reduce incidences of aflatoxin producers. Cluster amplification pattern (CAP) is a rapid multiplex PCR method for identifying and monitoring indels associated with atoxigenicity in A.flavus. Compared to previous techniques, the reported method allows for increased resolution, reduced cost, and greater speed in monitoring the stability of atoxigenic strains, incidences of indel mediated atoxigenicity and the structure of A.flavus populations. C1 [Callicott, K. A.; Cotty, P. J.] ARS, Food & Feed Safety Res Unit, USDA, Tucson, AZ USA. [Cotty, P. J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. RP Cotty, PJ (reprint author), Univ Arizona, Sch Plant Sci, POB 210036,Forbes Room 303, Tucson, AZ 85721 USA. EM pjcotty@email.arizona.edu FU ARS CRIS [5347-42000-020-00D] FX This work was supported by ARS CRIS # 5347-42000-020-00D. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 27 TC 3 Z9 3 U1 0 U2 13 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0266-8254 EI 1472-765X J9 LETT APPL MICROBIOL JI Lett. Appl. Microbiol. PD JAN PY 2015 VL 60 IS 1 BP 60 EP 65 DI 10.1111/lam.12337 PG 6 WC Biotechnology & Applied Microbiology; Microbiology SC Biotechnology & Applied Microbiology; Microbiology GA AW9PT UT WOS:000346590200009 PM 25274127 ER PT J AU Islamovic, E Garcia-Pedrajas, MD Chacko, N Andrews, DL Covert, SF Gold, SE AF Islamovic, Emir Garcia-Pedrajas, Maria D. Chacko, Nadia Andrews, David L. Covert, Sarah F. Gold, Scott E. TI Transcriptome Analysis of a Ustilago maydis ust1 Deletion Mutant Uncovers Involvement of Laccase and Polyketide Synthase Genes in Spore Development SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID PATHOGEN USTILAGO-MAYDIS; ASPERGILLUS-FUMIGATUS; MELANIN BIOSYNTHESIS; TELIOSPORE FORMATION; CRYPTOCOCCUS-NEOFORMANS; BINDING-PROTEIN; MAIZE; DIMORPHISM; CLUSTER; VIRULENCE AB Ustilago maydis, causal agent of corn smut disease, is a dimorphic fungus alternating between a saprobic budding haploid and an obligate pathogenic filamentous dikaryon. Maize responds to U. maydis colonization by producing tumorous structures, and only within these does the fungus sporulate, producing melanized sexual teliospores. Previously we identified Ust1, an APSES (Asmlp, Phdlp, Sok2p, Efg1p, and StuAp) transcription factor, whose deletion led to filamentous haploid growth and the production of highly pigmented teliospore-like structures in culture. In this study, we analyzed the transcriptome of a ust1 deletion mutant and functionally characterized two highly upregulated genes with potential roles in melanin biosynthesis: um05361, encoding a putative laccase (lac1), and um06414, encoding a polyketide synthase (pks1). The Delta lac1 mutant strains showed dramatically reduced virulence on maize seedlings and fewer, less-pigmented teliospores in adult plants. The Delta pks1 mutant was unaffected in seedling virulence but adult plant tumors generated hyaline, nonmelanized teliospores. Thus, whereas pks1 appeared to be restricted to the synthesis of melanin, lac1 showed a broader role in virulence. In conclusion, the ust1 deletion mutant provided an in vitro model for sporulation in U. maydis, and functional analysis supports the efficacy of this in vitro mutant analysis for identification of genes involved in in planta teliosporogenesis. C1 [Islamovic, Emir; Chacko, Nadia; Andrews, David L.; Gold, Scott E.] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. [Garcia-Pedrajas, Maria D.] Univ Malaga, Consejo Super Invest Cient IHSM UMA CSIC, Inst Hortofruticultura Subtrop & Mediterranea La, Estn Expt La Mayora, Malaga 29750, Spain. [Covert, Sarah F.] Univ Georgia, Warnell Sch Forestry & Nat Resources, Athens, GA 30602 USA. RP Gold, SE (reprint author), USDA ARS, Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. EM Scott.Gold@ars.usda.gov RI Garcia-Pedrajas, Maria /G-1729-2015; Chacko, Nadia/O-7834-2015 OI Garcia-Pedrajas, Maria /0000-0001-5520-1887; Chacko, Nadia/0000-0003-1817-2671 FU National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service [2006-35319-16738, GEO-2007-01605] FX This project was supported by the National Research Initiative of the United States Department of Agriculture Cooperative State Research, Education and Extension Service, grant numbers 2006-35319-16738 to S. E. Gold and S. F. Covert and GEO-2007-01605 to E. Islamovic. NR 49 TC 4 Z9 5 U1 2 U2 24 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD JAN PY 2015 VL 28 IS 1 BP 42 EP 54 DI 10.1094/MPMI-05-14-0133-R PG 13 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA AW4ZM UT WOS:000346286000004 PM 25226432 ER PT J AU Baeza-Montanez, L Gold, SE Espeso, EA Garcia-Pedrajas, MD AF Baeza-Montanez, Lourdes Gold, Scott E. Espeso, Eduardo A. Garcia-Pedrajas, Maria D. TI Conserved and Distinct Functions of the "Stunted" (StuA)-Homolog Ust1 During Cell Differentiation in the Corn Smut Fungus Ustilago maydis SO MOLECULAR PLANT-MICROBE INTERACTIONS LA English DT Article ID APSES TRANSCRIPTION FACTOR; DEPENDENT PROTEIN-KINASE; LOOP-HELIX PROTEIN; ASPERGILLUS-NIDULANS; CANDIDA-ALBICANS; FILAMENTOUS GROWTH; PATHOGENIC DEVELOPMENT; ELECTRON-MICROSCOPY; NUCLEAR MIGRATION; GENE-EXPRESSION AB Ustilago maydis, causal agent of corn smut, can proliferate saprobically in a yeast form but its infectious filamentous form is an obligate parasite. Previously, we showed that Ust1, the first APSES (Asmlp, Phdlp, Sok2p, Efg1p, and StuAp) transcription factor functionally characterized in the phylum Basidiomycota, controlled morphogenesis and virulence in this species. Here, we further analyzed Ust1 function using multiple experimental approaches and determined that i) Ust1 activity was able to partially reverse stuA-conidiophore defects in Aspergillus nidulans; ii) in U. maydis, normal development and virulence were strongly dependent on precise induction or repression of Ust1 activity; iii) consistent with its role as a transcription factor regulating multiple processes, Ust1 accumulated in the nucleus at various stages of the life cycle; iv) however, it was undetectable at specific stages of pathogenic growth, indicating that Ust1 repression is part of normal development in planta; v) StuA response elements upstream of the ust1 open reading frame exhibited affinity for U. maydis DNA-binding proteins; vi) however, loss of regulated ust1 transcription had minor phenotypic effects; and vii) Ust1 was subject to post-translational phosphorylation but is not a target of cAMP signaling. Thus, the broad functional conservation between Ust1 and Ascomycota APSES proteins does not extend to the mechanisms regulating their activity. C1 [Baeza-Montanez, Lourdes; Garcia-Pedrajas, Maria D.] Univ Malaga, Consejo Super Invest Cient IHSM UMA CSIC, Estn Expt La Mayora, Inst Hortofruticultura Subtrop & Mediterranea La, Malaga 29750, Spain. [Gold, Scott E.] USDA ARS, Russell Res Ctr, Toxicol & Mycotoxin Res Unit, Athens, GA 30605 USA. [Espeso, Eduardo A.] CSIC, Ctr Invest Biol, Dept Cellular & Mol Biol, Madrid 28040, Spain. RP Garcia-Pedrajas, MD (reprint author), Univ Malaga, Consejo Super Invest Cient IHSM UMA CSIC, Estn Expt La Mayora, Inst Hortofruticultura Subtrop & Mediterranea La, Malaga 29750, Spain. EM mariola@eelm.csic.es RI Garcia-Pedrajas, Maria /G-1729-2015; Espeso, Eduardo /C-5259-2011 OI Garcia-Pedrajas, Maria /0000-0001-5520-1887; Espeso, Eduardo /0000-0002-5873-6059 FU Spanish Ministry of Science and Innovation [AGL2009-13445]; CSIC; European Social Fund program FX This work was supported by the Spanish Ministry of Science and Innovation Grant Number AGL2009-13445 awarded to M. D. Garcia-Pedrajas. L. Baeza-Montanez was recipient of a JAE-Pre fellowship from CSIC, co-funded by the European Social Fund program. NR 82 TC 2 Z9 3 U1 2 U2 8 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0894-0282 EI 1943-7706 J9 MOL PLANT MICROBE IN JI Mol. Plant-Microbe Interact. PD JAN PY 2015 VL 28 IS 1 BP 86 EP 102 DI 10.1094/MPMI-07-14-0215-R PG 17 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Plant Sciences GA AW4ZM UT WOS:000346286000007 PM 25208341 ER PT J AU Liu, LJ Zinkgraf, M Petzold, HE Beers, EP Filkov, V Groover, A AF Liu, Lijun Zinkgraf, Matthew Petzold, H. Earl Beers, Eric P. Filkov, Vladimir Groover, Andrew TI The Populus ARBORKNOX1 homeodomain transcription factor regulates woody growth through binding to evolutionarily conserved target genes of diverse function SO NEW PHYTOLOGIST LA English DT Article DE chromatin immunoprecipitation sequencing (ChIP-seq); forest tree; Populus; transcriptional regulation; wood formation ID DIFFERENTIAL EXPRESSION ANALYSIS; SEQUENCE-ANALYSIS TOOLS; SHOOT APICAL MERISTEM; STEM-CELL IDENTITY; RNA-SEQ DATA; CHIP-SEQ; ARABIDOPSIS DEVELOPMENT; BIOCONDUCTOR PACKAGE; SIGNAL-TRANSDUCTION; ETHYLENE RESPONSE AB The class I KNOX homeodomain transcription factor ARBORKNOX1 (ARK1) is a key regulator of vascular cambium maintenance and cell differentiation in Populus. Currently, basic information is lacking concerning the distribution, functional characteristics, and evolution of ARK1 binding in the Populus genome. Here, we used chromatin immunoprecipitation sequencing (ChIP-seq) technology to identify ARK1 binding loci genome-wide in Populus. Computational analyses evaluated the distribution of ARK1 binding loci, the function of genes associated with bound loci, the effect of ARK1 binding on transcript levels, and evolutionary conservation of ARK1 binding loci. ARK1 binds to thousands of loci which are highly enriched proximal to the transcriptional start sites of genes of diverse functions. ARK1 target genes are significantly enriched in paralogs derived from the whole-genome salicoid duplication event. Both ARK1 and a maize (Zea mays) homolog, KNOTTED1, preferentially target evolutionarily conserved genes. However, only a small portion of ARK1 target genes are significantly differentially expressed in an ARK1 over-expression mutant. This study describes the functional characteristics and evolution of DNA binding by a transcription factor in an undomesticated tree, revealing complexities similar to those shown for transcription factors in model animal species. C1 [Liu, Lijun; Zinkgraf, Matthew; Groover, Andrew] US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. [Petzold, H. Earl; Beers, Eric P.] Virginia Tech, Dept Hort, Blacksburg, VA 24061 USA. [Filkov, Vladimir] Univ Calif Davis, Dept Comp Sci, Davis, CA 95618 USA. [Groover, Andrew] Univ Calif Davis, Dept Plant Biol, Davis, CA 95618 USA. RP Groover, A (reprint author), US Forest Serv, Pacific Southwest Res Stn, USDA, Davis, CA 95618 USA. EM agroover@fs.fed.us OI Groover, Andrew/0000-0002-6686-5774 FU NIH [S10RR029668, S10RR027303]; USDA NIFA [2011-67013-30062]; Office of Science (BER), US Department of Energy [DE-FG02-07ER64449] FX We thank Courtney Castle and Gayle Dupper for plant transformation and propagation, and Victor Missirian and Trevor Ramsey for contribution of custom Python scripts and pipelines. We thank Kathy Haiby, Rich Shuren, and Brian Stanton of Greenwood Resources for facilitating harvesting of cambium and developing wood/bark samples. This work used the Vincent J. Coates Genomics Sequencing Laboratory at UC Berkeley, supported by NIH S10 Instrumentation Grants S10RR029668 and S10RR027303. This work was supported by USDA NIFA Grant 2011-67013-30062 to A.G. and V.F., and by the Office of Science (BER), US Department of Energy, Grant no. DE-FG02-07ER64449 to E.P.B. NR 69 TC 12 Z9 12 U1 1 U2 27 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0028-646X EI 1469-8137 J9 NEW PHYTOL JI New Phytol. PD JAN PY 2015 VL 205 IS 2 BP 682 EP 694 DI 10.1111/nph.13151 PG 13 WC Plant Sciences SC Plant Sciences GA AW9NO UT WOS:000346584600025 PM 25377848 ER PT J AU Pfender, WF Coop, LB Seguin, SG Mellbye, ME Gingrich, GA Silberstein, TB AF Pfender, W. F. Coop, L. B. Seguin, S. G. Mellbye, M. E. Gingrich, G. A. Silberstein, T. B. TI Evaluation of the Ryegrass Stem Rust Model STEMRUST_G and Its Implementation as a Decision Aid SO PHYTOPATHOLOGY LA English DT Article DE Lolium perenne; model validation; Puccinia graminis subsp graminicola ID POTATO LATE BLIGHT; PERENNIAL RYEGRASS; SIMULATION-MODEL; LATEBLIGHT MODEL; SEED CROPS; DESCRIBE EPIDEMICS; WETNESS DURATION; PHASEOLUS BEANS; VALIDATION; DISEASE AB STEMRUST_G, a simulation model for epidemics of stem rust in perennial ryegrass grown to maturity as a seed crop, was validated for use as a heuristic tool and as a decision aid for disease management with fungicides. Multistage validation had been used in model creation by incorporating previously validated submodels for infection, latent period duration, sporulation, fungicide effects, and plant growth. Validation of the complete model was by comparison of model output with observed disease severities in 35 epidemics at nine location-years in the Pacific Northwest of the United States. We judge the model acceptable for its purposes, based on several tests. Graphs of modeled disease progress were generally congruent with plotted disease severity observations. There was negligible average bias in the 570 modeled-versus-observed comparisons across all data, although there was large variance in size of the deviances. Modeled severities were accurate in >80% of the comparisons, where accuracy is defined as the modeled value being within twice the 95% confidence interval of the observed value, within +/- 1 day of the observation date. An interactive website was created to produce disease estimates by running STEMRUST_G with user-supplied disease scouting information and automated daily weather data inputs from field sites. The model and decision aid supplement disease managers' information by estimating the level of latent (invisible) and expressed disease since the last scouting observation, given season-long weather conditions up to the present, and it estimates effects of fungicides on epidemic development. In additional large-plot experiments conducted in grower fields, the decision aid produced disease management outcomes (management cost and seed yield) as good as or better than the growers' standard practice. In future, STEMRUST_G could be modified to create similar models and decision aids for stem rust of wheat and barley, after additional experiments to determine appropriate parameters for the disease in these small-grain hosts. C1 [Pfender, W. F.] ARS, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. Oregon State Univ, Dept Crop Sci, Corvallis, OR 97331 USA. Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Pfender, WF (reprint author), ARS, USDA, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM pfenderw@onid.orst.edu NR 38 TC 0 Z9 0 U1 2 U2 10 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JAN PY 2015 VL 105 IS 1 BP 35 EP 44 DI 10.1094/PHYTO-06-14-0156-R PG 10 WC Plant Sciences SC Plant Sciences GA AW6FG UT WOS:000346364400004 PM 25098496 ER PT J AU Pfender, WF Upper, D AF Pfender, W. F. Upper, D. TI A Simulation Model for Epidemics of Stem Rust in Ryegrass Seed Crops SO PHYTOPATHOLOGY LA English DT Article DE azoxystrobin; decision aid; Lolium perenne; propiconazole; wheat ID PERENNIAL RYEGRASS; PUCCINIA-RECONDITA; DISEASE MANAGEMENT; DESCRIBE EPIDEMICS; PHASEOLUS BEANS; WHEAT; SPORULATION; VALIDATION; PHENOLOGY; SEVERITY AB A simulation model (STEMRUST_G, named for stem rust of grasses) was created for stem rust (caused by Puccinia graminis subsp. graminicola) in perennial ryegrass grown to maturity as a seed crop. The model has a daily time step and is driven by weather data and an initial input of disease severity from field observation. Key aspects of plant growth are modeled. Disease severity is modeled as rust population growth, where individuals are pathogen colonies (pustules) grouped in cohorts defined by date of initiation and plant part infected. Infections due to either aerial spread or within-plant contact spread are modeled. Pathogen cohorts progress through life stages that are modeled as disease cycle components (colony establishment, latent period, infectious period, and sporulation) affected by daily weather variables, plant growth, and fungicide application. Fungicide effects on disease cycle components are modeled for two commonly used active ingredients, applied preinfection or postinfection. Previously validated submodels for certain disease cycle components formed the framework for integrating additional processes, and the complete model was calibrated with field data from 10 stem rust epidemics. Discrepancies between modeled outcomes and the calibration data (log(10)[modeled] - log(10)[observed]) had a mean near zero but considerable variance, with 1 standard deviation = 0.5 log(10) units (3.2-fold). It appears that a large proportion of the modeling error variance may be due to variability in field observations of disease severity. An action threshold for fungicide application was derived empirically, using a constructed weather input file favorable for disease development. The action threshold is a negative threshold, representing a level of disease (latent plus visible) below which damaging levels of disease are unable to develop before the yield-critical crop stage. The model is in the public domain and available on the Internet. C1 [Pfender, W. F.] ARS, USDA, Natl Forage Seed Prod Res Ctr, Corvallis, OR 97331 USA. Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. RP Pfender, WF (reprint author), ARS, USDA, Natl Forage Seed Prod Res Ctr, 3450 SW Campus Way, Corvallis, OR 97331 USA. EM pfenderw@onid.orst.edu NR 33 TC 0 Z9 0 U1 2 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JAN PY 2015 VL 105 IS 1 BP 45 EP 56 DI 10.1094/PHYTO-03-14-0068-R PG 12 WC Plant Sciences SC Plant Sciences GA AW6FG UT WOS:000346364400005 PM 25098493 ER PT J AU Short, DPG Gurung, S Koike, ST Klosterman, SJ Subbarao, KV AF Short, D. P. G. Gurung, S. Koike, S. T. Klosterman, S. J. Subbarao, K. V. TI Frequency of Verticillium Species in Commercial Spinach Fields and Transmission of V. dahliae from Spinach to Subsequent Lettuce Crops SO PHYTOPATHOLOGY LA English DT Article ID LACTUCA-SATIVA; PLANT-DISEASE; WILT; SEED; PATHOGEN; SOIL; WHEAT; COLONIZATION; EPIDEMIOLOGY; RESISTANCE AB Verticillium wilt caused by V. dahliae is a devastating disease of lettuce in California (CA). The disease is currently restricted to a small geographic area in central coastal CA, even though cropping patterns in other coastal lettuce production regions in the state are similar. Infested spinach seed has been implicated in the introduction of V. dahliae into lettuce fields but direct evidence linking this inoculum to wilt epidemics in lettuce is lacking. In this study, 100 commercial spinach fields in four coastal CA counties were surveyed to evaluate the frequency of Verticillium species recovered from spinach seedlings and the area under spinach production in each county was assessed. Regardless of the county, V. isaacii was the most frequently isolated species from spinach followed by V. dahliae and, less frequently, V. klebahnii. The frequency of recovery of Verticillium species was unrelated to the occurrence of Verticillium wilt on lettuce in the four counties but was related to the area under spinach production in individual counties. The transmission of V. dahliae from infested spinach seeds to lettuce was investigated in microplots. Verticillium wilt developed on lettuce following two or three plantings of Verticillium-infested spinach, in independent experiments. The pathogen recovered from the infected lettuce from microplots was confirmed as V. dahliae by polymerase chain reaction assays. In a greenhouse study, transmission of a green fluorescence protein-tagged mutant strain of V. dahliae from spinach to lettuce roots was demonstrated, after two cycles of incorporation of infected spinach residue into the soil. This study presents conclusive evidence that V. dahliae introduced via spinach seed can cause Verticillium wilt in lettuce. C1 [Short, D. P. G.; Gurung, S.; Subbarao, K. V.] Univ Calif Davis, Dept Plant Pathol, US Dept Agr Res Stn, Salinas, CA 93905 USA. [Koike, S. T.] Univ Calif Cooperat Extens, Salinas, CA 93901 USA. [Klosterman, S. J.] ARS, USDA, Salinas, CA 93905 USA. RP Subbarao, KV (reprint author), Univ Calif Davis, Dept Plant Pathol, US Dept Agr Res Stn, 1636 E Alisal St, Salinas, CA 93905 USA. EM kvsubbarao@ucdavis.edu FU USDA-NIFA-SCRI grant [2010-51181-21069]; California Leafy Greens Research Board FX Funding for this study was provided by USDA-NIFA-SCRI grant no. 2010-51181-21069 and the California Leafy Greens Research Board. We thank S. Benzen, A. Anchieta, P. Ayala, K. Kammeijer, L. Ochoa, D. Renteria, C. Marchebout, and R. Marchebout for their excellent technical assistance. NR 39 TC 5 Z9 5 U1 5 U2 21 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JAN PY 2015 VL 105 IS 1 BP 80 EP 90 DI 10.1094/PHYTO-02-14-0046-R PG 11 WC Plant Sciences SC Plant Sciences GA AW6FG UT WOS:000346364400008 PM 25098494 ER PT J AU Bonde, MR Murphy, CA Bauchan, GR Luster, DG Palmer, CL Nester, SE Revell, JM Berner, DK AF Bonde, M. R. Murphy, C. A. Bauchan, G. R. Luster, D. G. Palmer, C. L. Nester, S. E. Revell, J. M. Berner, D. K. TI Evidence for Systemic Infection by Puccinia horiana, Causal Agent of Chrysanthemum White Rust, in Chrysanthemum SO PHYTOPATHOLOGY LA English DT Article ID DISEASES; FUNGUS; ELM AB Puccinia horiana, causal agent of the disease commonly known as chrysanthemum white rust (CWR), is a quarantine-significant fungal pathogen of chrysanthemum in the United States and indigenous to Asia. The pathogen was believed to have been eradicated in the United States but recently reappeared on several occasions in northeastern United States. The objective of the study presented here was to determine whether P. horiana could systemically infect chrysanthemum plants, thus providing a means of survival through winters. Scanning and transmission electron microscopy revealed the development of P. horiana on the surface and within leaves, stems, or crowns of inoculated chrysanthemum plants artificially exposed to northeastern U.S. winter temperatures. P. horiana penetrated leaves directly through the cuticle and then colonized the mesophyll tissue both inter-and intracellularly. An electron-dense material formed at the interface between fungal and host mesophyll cells, suggesting that the pathogen adhered to the plant cells. P. horiana appeared to penetrate mesophyll cell walls by enzymatic digestion, as indicated by the absence of deformation lines in host cell walls at penetration sites. The fungus was common in vascular tissue within the infected crown, often nearly replacing the entire contents of tracheid cell walls. P. horiana frequently passed from one tracheid cell to an adjacent tracheid cell by penetration either through pit pairs or nonpitted areas of the cell walls. Individual, presumed, fungal cells in mature tracheid cells of the crown and stems arising from infected crowns suggested that the pathogen might have been moving at least partially by means of the transpiration stream. The demonstration that chrysanthemum plants can be systemically infected by P. horiana suggests that additional disease control measures are required to effectively control CWR. C1 [Bonde, M. R.; Nester, S. E.; Berner, D. K.] ARS, USDA, Ft Detrick, MD 21702 USA. [Murphy, C. A.; Bauchan, G. R.] ARS, USDA, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Palmer, C. L.; Revell, J. M.] Rutgers State Univ, IR Project 4, Princeton, NJ 08520 USA. RP Bonde, MR (reprint author), ARS, USDA, 1301 Ditto Ave, Ft Detrick, MD 21702 USA. EM Morris.bonde@ars.usda.gov FU USDA-APHIS-CPHST FX Funding for this research was partially supported by the USDA-APHIS-CPHST through the FY2011 and FY2012 Farm Bills. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. NR 26 TC 4 Z9 4 U1 1 U2 9 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0031-949X EI 1943-7684 J9 PHYTOPATHOLOGY JI Phytopathology PD JAN PY 2015 VL 105 IS 1 BP 91 EP 98 DI 10.1094/PHYTO-09-13-0266-R PG 8 WC Plant Sciences SC Plant Sciences GA AW6FG UT WOS:000346364400009 PM 25121642 ER PT J AU Jones, R Chambers, JC Johnson, DW Blank, RR Board, DI AF Jones, Rachel Chambers, Jeanne C. Johnson, Dale W. Blank, Robert R. Board, David I. TI Effect of repeated burning on plant and soil carbon and nitrogen in cheatgrass (Bromus tectorum) dominated ecosystems SO PLANT AND SOIL LA English DT Article DE Cold desert; Invasive annual grasses; Repeated fire; Restoration; Sagebrush; Shrublands ID GREAT-BASIN; SAGEBRUSH-STEPPE; TALLGRASS PRAIRIE; PINE FOREST; LONG-TERM; FIRE TEMPERATURES; PRESCRIBED FIRE; ARID GRASSLAND; ORGANIC-CARBON; L. INVASION AB Fire has profound effects on ecosystem properties, but few studies have addressed the effect of repeated burns on soil nutrients, and none have been conducted in cold desert ecosystems where invasion by exotic annual grasses is resulting in greater fire frequency. In a 5 year study, we examined effects of repeated burning, litter removal, and post-fire seeding on carbon (C) and nitrogen (N) contents in soils, litter, and vegetation in a cheatgrass-dominated Wyoming big sagebrush ecological type. We developed a multivariate model to identify potential mechanisms influencing treatment effects and examine the influence of environmental factors such as precipitation and temperature. We found that repeated burning had strong negative effects on litter C and N contents, but did not reduce soil nutrients or vegetation C and N contents, likely due to cool fire temperatures. There were few effects of litter removal or post-fire seeding. Instead, precipitation and temperature interacted with burning and had the strongest influences on soil N and vegetation C and N contents over time. Management strategies aimed at decreasing litter and seed banks and increasing competitive interactions may be more effective at reducing cheatgrass success than approaches for reducing soil nutrients. C1 [Jones, Rachel] Univ Nevada, Dept Nat Resources & Environm Sci, Ecol Evolut & Conservat Biol Grad Grp, Reno, NV 89512 USA. [Chambers, Jeanne C.; Board, David I.] US Forest Serv, Rocky Mt Res Stn, Reno, NV 89512 USA. [Johnson, Dale W.] Univ Nevada, Dept Nat Resources & Environm Sci, Reno, NV 89512 USA. [Blank, Robert R.] ARS, USDA, Reno, NV 89512 USA. RP Jones, R (reprint author), Univ Nevada, Dept Nat Resources & Environm Sci, Ecol Evolut & Conservat Biol Grad Grp, 1000 Valley Rd, Reno, NV 89512 USA. EM rachel.o.jones@gmail.com; jchambers@fs.fed.us; dwj@cabnr.unr.edu; Bob.Blank@ars.usda.gov; dboard@fs.fed.us FU Rocky Mountain Research Station FX This study was a collaborative effort among the USFS Rocky Mountain Research Station, University of Nevada, Reno, USDA Agricultural Research Service and Winnemucca District of the Nevada Bureau of Land Management. Research funding was provided through the Rocky Mountain Research Station. We thank T. Morgan, C. Rosner, C. Dencker, and a large number of summer technicians for valuable assistance in the field and lab, and B. Leger, P. Verburg, T. Albright, and B. Rau for valuable comments on earlier drafts of this manuscript. NR 74 TC 8 Z9 8 U1 10 U2 100 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD JAN PY 2015 VL 386 IS 1-2 BP 47 EP 64 DI 10.1007/s11104-014-2242-2 PG 18 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AW8DN UT WOS:000346491000004 ER PT J AU Ingram, LJ Schuman, GE Parkin, TB Mortenson, M AF Ingram, L. J. Schuman, G. E. Parkin, T. B. Mortenson, M. TI Trace gas fluxes from a Northern mixed-grass prairie interseeded with Alfalfa SO PLANT AND SOIL LA English DT Article DE Alfalfa; Lucerne; Legume; Nitrous oxide; Methane; Semi-arid ID NITROUS-OXIDE EMISSIONS; SATIVA SSP FALCATA; FILLED PORE-SPACE; N2O EMISSIONS; GREAT-PLAINS; SHORTGRASS STEPPE; METHANE EMISSIONS; GRAZED GRASSLAND; CARBON-DIOXIDE; INNER-MONGOLIA AB That legumes improve soil fertility is well established, however the extent that N fixed by legumes may increase trace gas emissions is poorly understood. Our aim was to determine if greater soil N in native prairies interseeded with alfalfa would increase trace gas emissions of nitrous oxide (N2O) and methane (CH4). A chronosequence study to investigate interseeded alfalfa on the fluxes of N2O and CH4 was undertaken on three prairie sites. At each site, native grasslands interseeded with alfalfa for 38, 16 and 5 years, along with adjacent native grassland controls and native grasslands fertilized with a one-time addition of N fertilizer, were measured for NO2 and CH4 using static chambers. Across interseeding years there were few significant differences between the three treatments (Native, Alfalfa, Fertilized). Despite increased soil N, the interseeded treatment released no more N2O than native grasslands. Methane uptake was generally greatest on interseeded grasslands and least on fertilized grasslands. Our results suggest that while alfalfa can increase soil N and improve productivity, it did not increase trace gas production relative to native grasslands. This is a win-win situation for ranchers wishing to increase soil fertility and thus their overall productivity and forage quality without increasing trace gas emissions. C1 [Ingram, L. J.] Univ Wyoming, Dept Renewable Resources, Laramie, WY 82071 USA. [Ingram, L. J.; Schuman, G. E.; Mortenson, M.] ARS, USDA, High Plains Grasslands Res Stn, Cheyenne, WY 82009 USA. [Parkin, T. B.] ARS, USDA, Natl Lab Agr & Environm, Ames, IA 50011 USA. [Ingram, L. J.] Univ Sydney, Fac Agr & Environm, Ctr Carbon Water & Food, Brownlow Hill, NSW 2570, Australia. RP Ingram, LJ (reprint author), Univ Sydney, Fac Agr & Environm, Ctr Carbon Water & Food, Brownlow Hill, NSW 2570, Australia. EM lachlan.ingram@sydney.edu.au RI Ingram, Lachlan/E-3529-2013 OI Ingram, Lachlan/0000-0002-4697-9504 FU USDA-Agricultural Research Service under the ARS GRACEnet Project FX We would also like to acknowledge Otis Smith, Jr. for technical assistance in sampling and analysis of gas samples. We appreciate and acknowledge the comments of a number of anonymous reviewers thoughtful comments that resulted in improving this article. This publication is based upon research supported by the USDA-Agricultural Research Service under the ARS GRACEnet Project. Mention of specific products is solely for the benefit of the reader and does not imply endorsement by the USDA-ARS NR 63 TC 1 Z9 1 U1 1 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0032-079X EI 1573-5036 J9 PLANT SOIL JI Plant Soil PD JAN PY 2015 VL 386 IS 1-2 BP 285 EP 301 DI 10.1007/s11104-014-2264-9 PG 17 WC Agronomy; Plant Sciences; Soil Science SC Agriculture; Plant Sciences GA AW8DN UT WOS:000346491000021 ER PT J AU Nar, M Webber, C D'Souza, NA AF Nar, Mangesh Webber, Charles, III D'Souza, Nandika Anne TI Rigid Polyurethane and Kenaf Core Composite Foams SO POLYMER ENGINEERING AND SCIENCE LA English DT Article ID NANOCOMPOSITE FOAMS; NATURAL FIBERS; OIL; FILLER AB Rigid PU foams are valuable in many construction applications. Kenaf is a bast fiber plant where the surface stem skin provides bast fibers whose strength-to-weight ratio competes with glass fiber. The higher volume product of the kenaf core is an under-investigated area in composite applications. The naturally porous structure of kenaf-core provides a novel reinforcement particle. In this work, foams of rigid polyurethane with 5, 10, and 15% kenaf-core were formed. To date efforts at using it as a reinforcement have proven largely unsuccessful. This was mirrored in this effort when free expansion of the foam was utilized. However, introducing constraint during foaming resulted in reinforcement. The environmental scanning electron microscopy is used in conjunction with in-situ microCT compression to capture the change in void fractions before and after deformation. The results show that free foaming resulted in poor reinforcement while a constrained expansion on the foam increased the reinforcement potential of the kenaf core. POLYM. ENG. SCI., 55:132-144, 2015. (c) 2014 Society of Plastics Engineers C1 [Nar, Mangesh; D'Souza, Nandika Anne] Univ N Texas, Dept Mat Sci & Engn, Denton, TX 76203 USA. [Webber, Charles, III] ARS, USDA, Sugarcane Res Unit, Houma, LA 70360 USA. [D'Souza, Nandika Anne] Univ N Texas, Dept Mech & Energy Engn, Denton, TX 76203 USA. RP D'Souza, NA (reprint author), Univ N Texas, Dept Mat Sci & Engn, 1155 Union Circle 305310, Denton, TX 76203 USA. EM nandika.dsouza@unt.edu FU NSF-PFI [1114389]; NSF-CMMI [1031828] FX We acknowledge NSF-PFI 1114389 and NSF-CMMI 1031828 for financial support to fulfill this work. NR 32 TC 2 Z9 2 U1 8 U2 26 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0032-3888 EI 1548-2634 J9 POLYM ENG SCI JI Polym. Eng. Sci. PD JAN PY 2015 VL 55 IS 1 BP 132 EP 144 DI 10.1002/pen.23868 PG 13 WC Engineering, Chemical; Polymer Science SC Engineering; Polymer Science GA AW4QQ UT WOS:000346266200016 ER PT J AU Rao, RSP Moller, IM Thelen, JJ Miernyk, JA AF Rao, R. Shyama Prasad Moller, Ian Max Thelen, Jay J. Miernyk, Jan A. TI Convergent signaling pathways-interaction between methionine oxidation and serine/threonine/tyrosine O-phosphorylation SO CELL STRESS & CHAPERONES LA English DT Review DE Methionine sulfoxide; O-phosphorylation; Oxidative stress; Reactive oxygen species; Signaling ID SEED DORMANCY; PROTEIN CARBONYLATION; SULFOXIDE REDUCTASE; ARABIDOPSIS-THALIANA; ANTIOXIDANT DEFENSE; GERMINATION; ACTIVATION; RESIDUES; STRESS; QUANTIFICATION AB Oxidation of methionine (Met) to Met sulfoxide (MetSO) is a frequently found reversible posttranslational modification. It has been presumed that the major functional role for oxidation-labile Met residues is to protect proteins/cells from oxidative stress. However, Met oxidation has been established as a key mechanism for direct regulation of a wide range of protein functions and cellular processes. Furthermore, recent reports suggest an interaction between Met oxidation and O-phosphorylation. Such interactions are a potentially direct interface between redox sensing and signaling, and cellular protein kinase/phosphatase-based signaling. Herein, we describe the current state of Met oxidation research, provide some mechanistic insight into crosstalk between these two major posttranslational modifications, and consider the evolutionary significance and regulatory potential of this crosstalk. C1 [Rao, R. Shyama Prasad; Thelen, Jay J.; Miernyk, Jan A.] Univ Missouri, Div Biochem, Columbia, MO 65211 USA. [Moller, Ian Max] Aarhus Univ, Fac Sci & Technol, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark. [Miernyk, Jan A.] Univ Missouri, ARS, Plant Genet Res Unit, USDA, Columbia, MO 65211 USA. RP Miernyk, JA (reprint author), Univ Missouri, Div Biochem, Columbia, MO 65211 USA. EM drrsprao@gmail.com; ian.max.moller@agrsci.dk; thelenj@missouri.edu; miernykj@missouri.edu RI Rao, R Shyama Prasad/E-5434-2011 OI Rao, R Shyama Prasad/0000-0002-2285-6788 FU National Science Foundation Plant Genome Research Program [DBI-0604439]; Danish Council for Independent Research-Natural Sciences FX Research in the Thelen Lab is supported by a grant from the National Science Foundation Plant Genome Research Program Grant No. DBI-0604439 and research in the Moller lab was supported by a grant from the Danish Council for Independent Research-Natural Sciences. NR 64 TC 6 Z9 6 U1 1 U2 18 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 1355-8145 EI 1466-1268 J9 CELL STRESS CHAPERON JI Cell Stress Chaperones PD JAN PY 2015 VL 20 IS 1 BP 15 EP 21 DI 10.1007/s12192-014-0544-1 PG 7 WC Cell Biology SC Cell Biology GA AW0PG UT WOS:000345994600003 PM 25238876 ER PT J AU Davis, TA Fiorotto, ML Suryawan, A AF Davis, Teresa A. Fiorotto, Marta L. Suryawan, Agus TI Bolus vs. continuous feeding to optimize anabolism in neonates SO CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE LA English DT Review DE amino acids; infant; protein synthesis; skeletal muscle; translation initiation ID MUSCLE PROTEIN-SYNTHESIS; SKELETAL-MUSCLE; PRETERM INFANTS; AMINO-ACIDS; INTERMITTENT BOLUS; LEUCINE SUPPLEMENTATION; VISCERAL TISSUES; COMPLEX 1; PIGS; MTORC1 AB Purpose of review Neonates with feeding difficulties can be fed by orogastric tube, using either continuous or bolus delivery. This review reports on recent findings that bolus is advantageous compared to continuous feeding in supporting optimal protein anabolism. Recent findings Whether bolus or continuous feeding is more beneficial has been controversial, largely due to limitations inherent in clinical studies, such as the presence of confounding variables and the inability to use invasive approaches. Recent studies using the piglet as a model of the human neonate showed that, compared to continuous feeding, bolus feeding enhances protein synthesis and promotes greater protein deposition. The increase in protein synthesis occurs in muscles of varying fiber type and in visceral tissues whereas muscle protein degradation is largely insensitive to feeding pattern. This higher protein synthesis rate is enabled by the rapid and profound increases in circulating amino acids and insulin that occur following a bolus feed, which activate the intracellular signaling pathways leading to mRNA translation. Summary Recent findings indicate that bolus feeding enhances protein synthesis more than continuous feeding and promotes greater protein anabolism. The difference in response is attributable to the pulsatile pattern of amino acid-induced and insulin-induced translation initiation induced only by bolus feeding. C1 [Davis, Teresa A.; Fiorotto, Marta L.; Suryawan, Agus] ARS, Dept Pediat, USDA, Childrens Nutr Res Ctr,Baylor Coll Med, Houston, TX 77030 USA. RP Davis, TA (reprint author), Baylor Coll Med, Houston, TX 77030 USA. EM tdavis@bcm.edu FU National Institute of Arthritis and Musculoskeletal and Skin Diseases [AR-044474, AR-46308]; National Institute of Child Health and Human Development [HD-072891]; United States Department of Agriculture National Institute of Agriculture [2013-67015-20438]; USDA/ARS [6250-510000-055] FX The work was supported by National Institute of Arthritis and Musculoskeletal and Skin Diseases Grants AR-044474 (T.A.D.) and AR-46308 (M.L.F.), National Institute of Child Health and Human Development HD-072891 (T.A.D.), United States Department of Agriculture National Institute of Agriculture grant 2013-67015-20438 (T.A.D.), and by the USDA/ARS under Cooperative Agreement no. 6250-510000-055 (T.A.D.). This work is a publication of the USDA, Agricultural Research Service (USDA/ARS) Children's Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, TX. NR 40 TC 2 Z9 2 U1 1 U2 5 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA 530 WALNUT ST, PHILADELPHIA, PA 19106-3621 USA SN 1363-1950 EI 1473-6519 J9 CURR OPIN CLIN NUTR JI Curr. Opin. Clin. Nutr. Metab. Care PD JAN PY 2015 VL 18 IS 1 BP 102 EP 108 DI 10.1097/MCO.0000000000000128 PG 7 WC Endocrinology & Metabolism; Nutrition & Dietetics SC Endocrinology & Metabolism; Nutrition & Dietetics GA AW2MG UT WOS:000346121700016 PM 25474017 ER PT J AU Wang, XZ Jiang, GL Song, QJ Cregan, PB Scott, RA Zhang, JP Yen, Y Brown, M AF Wang, Xianzhi Jiang, Guo-Liang Song, Qijian Cregan, Perry B. Scott, Roy A. Zhang, Jiaoping Yen, Yang Brown, Michael TI Quantitative trait locus analysis of seed sulfur-containing amino acids in two recombinant inbred line populations of soybean SO EUPHYTICA LA English DT Article DE Soybean; Amino acids; Cysteine; Methionine; Quantitative trait loci (QTLs); Molecular breeding ID CYSTATHIONINE GAMMA-SYNTHASE; SUDDEN-DEATH SYNDROME; LINKAGE GROUP-I; AGRONOMIC TRAITS; FIELD-RESISTANCE; QTL ANALYSIS; SSR MARKERS; OIL CONTENT; PROTEIN; IDENTIFICATION AB Soybean (Glycine max (L.) Merr.) is a major source of plant protein for humans and livestock. Deficiency of sulfur-containing amino acids (cysteine and methionine) in soybean protein is a main limitation of soybean meal as an animal feed ingredient. The objectives of this study were to identify and validate quantitative trait loci (QTLs) associated with cysteine and methionine contents in two recombinant inbred line (RIL) populations, and to analyze the genetic effects of individual QTLs and QTL combinations in soybean. Both the mapping population of SD02-4-59 x A02-381100 and validation population of SD02-911 x SD00-1501 were evaluated for cysteine and methionine contents in multiple environments. Correlation analysis indicated that there was a highly positive correlation between cysteine and methionine contents. Significant positive correlations were also observed between the sulfur-containing amino acid contents and protein content. In the mapping population, eight QTLs for both cysteine and methionine contents were consistently detected in any individual environment and the average data over all three environments. Three of these QTLs were confirmed in the validation population. A comparison with the previous studies indicated that most of the genomic regions linked to the QTLs for the sulfur-containing amino acids were also associated with protein content. Cumulative effects of multiple QTLs for the traits were significant in both populations. This information should be useful for the improvement of the levels of protein and amino acids in soybean seeds. C1 [Wang, Xianzhi; Jiang, Guo-Liang; Zhang, Jiaoping] S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. [Wang, Xianzhi] Yunnan Univ, Coll Agron, Kunming, Yunnan, Peoples R China. [Jiang, Guo-Liang] Virginia State Univ, Agr Res Stn, Petersburg, VA 23806 USA. [Song, Qijian; Cregan, Perry B.] ARS, Soybean Genom & Improvement Lab, USDA, Beltsville, MD 20705 USA. [Scott, Roy A.] ARS, GWCC BLTSVL, USDA, Beltsville, MD 20705 USA. [Yen, Yang] S Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA. [Brown, Michael] S Dakota State Univ, Dept Nat Resource Management, Brookings, SD 57007 USA. RP Jiang, GL (reprint author), S Dakota State Univ, Dept Plant Sci, Brookings, SD 57007 USA. EM gjiang@vsu.edu FU South Dakota Agricultural Experiment Station; USDA-NIFA/CSREES Hatch project; USB Seed Composition Research project via USDA/ARS; South Dakota Soybean Research & Promotion Council FX This research was supported in part by the South Dakota Agricultural Experiment Station and USDA-NIFA/CSREES Hatch project, and the grants of USB Seed Composition Research project via USDA/ARS and South Dakota Soybean Research & Promotion Council project awarded to Guo-Liang Jiang. The authors are grateful to Dr. David Hyten for assistance in SNP genotyping and Dr. Kristin Bilyeu for GmFAD3 genotyping. Thanks also go to Marci Green, Siddhi Bhusal, Nicholas Hall and Sivananda Tirumalaraju for assistance in field experiments. NR 46 TC 1 Z9 1 U1 4 U2 27 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0014-2336 EI 1573-5060 J9 EUPHYTICA JI Euphytica PD JAN PY 2015 VL 201 IS 2 BP 293 EP 305 DI 10.1007/s10681-014-1223-0 PG 13 WC Agronomy; Plant Sciences; Horticulture SC Agriculture; Plant Sciences GA AW3IP UT WOS:000346180000009 ER PT J AU Seipel, T Alexander, JM Daehler, CC Rew, LJ Edwards, PJ Dar, PA McDougall, K Naylor, B Parks, C Pollnac, FW Reshi, ZA Schroder, M Kueffer, C AF Seipel, Tim Alexander, Jake M. Daehler, Curtis C. Rew, Lisa J. Edwards, Peter J. Dar, Pervaiz A. McDougall, Keith Naylor, Bridgett Parks, Catherine Pollnac, Fredric W. Reshi, Zafar A. Schroder, Mel Kueffer, Christoph TI Performance of the herb Verbascum thapsus along environmental gradients in its native and non-native ranges SO JOURNAL OF BIOGEOGRAPHY LA English DT Article DE Biennial herb; climate; elevation; invasion; invasive plants; niche model; non-native; phenotypic plasticity; Verbascum ID LIFE-HISTORY VARIATION; INTRODUCED PLANT-POPULATIONS; COMMON MULLEIN; SPECIES DISTRIBUTIONS; INVASIVE PLANT; EVOLUTION; CLINES; LARGER; SCROPHULARIACEAE; GERMINATION AB AimWe evaluated whether the performance of individuals and populations of the invasive plant Verbascum thapsus differs between its native and non-native ranges, across climate gradients, and in response to its position in a global-scaled niche model. LocationIndia (Kashmir) and Switzerland (native range) and Australia and USA (Hawaii, Montana and Oregon) (non-native range). MethodsWe measured population characteristics (density of flowering individuals, population size), plant traits (plant height, number of flowering branches) and seed germination for 50 populations of V. thapsus growing along elevational gradients in six mountain regions around the world (two in the native range, and four in the non-native range). We fitted linear regression models to determine the relationship of plant and population level performance variables to range, region, climate and probability of occurrence from a global niche model. ResultsPlant height, number of flowering branches and population density of V. thapsus did not differ consistently between the native and non-native ranges, but the area covered by populations in the non-native range was on average two orders of magnitude larger than the area of native populations. Within and among regions, individual plant performance traits responded variably to precipitation and mean annual temperature, depending on the climatic range of the observed populations; however, performance was greater for populations that had a greater modelled probability of occurrence. Seed germination under controlled conditions was highest between 20 and 35 degrees C and consistent across populations, although germination at low temperatures was substantially higher for native populations from Kashmir. Main conclusionsThe introduction of V. thapsus to its non-native range is not associated with consistent increases in plant performance. Instead, plant performance is more strongly influenced by position within the climate niche of the species, position along environmental gradients, and climatic or other differences among regions. We demonstrate that individual-level and population-level performance traits can yield different predictors of plant performance. Therefore, future studies comparing plant performance in native and non-native ranges should include both plant and population characteristics and should also sample the target species from multiple regions in both ranges and along environmental gradients that comprehensively represent the niche of the species. C1 [Seipel, Tim; Alexander, Jake M.; Edwards, Peter J.; Kueffer, Christoph] Swiss Fed Inst Technol, Inst Integrat Biol, CH-8092 Zurich, Switzerland. [Seipel, Tim; Rew, Lisa J.; Pollnac, Fredric W.] Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. [Daehler, Curtis C.] Univ Hawaii, Dept Bot, Honolulu, HI 96822 USA. [Dar, Pervaiz A.; Reshi, Zafar A.] Univ Kashmir, Dept Bot, Ctr Biodivers & Taxon, Srinagar 190006, Jammu & Kashmir, India. [McDougall, Keith; Schroder, Mel] Off Environm & Heritage, Queanbeyan, NSW 2620, Australia. [Naylor, Bridgett; Parks, Catherine] US Forest Serv, USDA, Pacific NW Res Stn, La Grande, OR 97850 USA. RP Seipel, T (reprint author), Montana State Univ, Dept Land Resources & Environm Sci, Bozeman, MT 59717 USA. EM tim.seipel@gmail.com RI Alexander, Jake/P-2580-2014 OI Alexander, Jake/0000-0003-2226-7913 FU Swiss Federal Institute of Technology (ETH Zurich) while at the Institute of Integrative Biology, ETH Zurich, Switzerland; NRI-CSREES Award while at Montana State University, MT, USA [2009-55320-05033] FX T.S. was funded by a research grant from the Swiss Federal Institute of Technology (ETH Zurich) while at the Institute of Integrative Biology, ETH Zurich, Switzerland, and by NRI-CSREES Award Number 2009-55320-05033 while at Montana State University, MT, USA. We thank Catherine McDougall, Jackie Miles, Catherine Pickering, Kent Coe and Neville Walsh for assistance with fieldwork and reconnaissance, and Meinrad Kuttel from BAFU - Swiss Biodiversity Monitoring (http://www.biodiversitymonitoring.ch/) for sharing data on the spatial distribution of V. thapsus in Switzerland. NR 70 TC 3 Z9 3 U1 16 U2 87 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0305-0270 EI 1365-2699 J9 J BIOGEOGR JI J. Biogeogr. PD JAN PY 2015 VL 42 IS 1 BP 132 EP 143 DI 10.1111/jbi.12403 PG 12 WC Ecology; Geography, Physical SC Environmental Sciences & Ecology; Physical Geography GA AW1SH UT WOS:000346069700013 ER PT J AU Zhu, YC Luttrell, R AF Zhu, Yu Cheng Luttrell, Randall TI Altered gene regulation and potential association with metabolic resistance development to imidacloprid in the tarnished plant bug, Lygus lineolaris SO PEST MANAGEMENT SCIENCE LA English DT Article DE cDNA; microarray; P450; esterase; imidacloprid; resistance; susceptibility; tarnished plant bug; Lygus lineolaris ID BEMISIA-TABACI HEMIPTERA; NEONICOTINOID INSECTICIDES; NILAPARVATA-LUGENS; CROSS-RESISTANCE; BROWN PLANTHOPPER; DIPTERA MUSCIDAE; OVER-EXPRESSION; MYZUS-PERSICAE; ALEYRODIDAE; ESTERASE AB BACKGROUND: Chemical spray on cotton is almost an exclusive method for controlling tarnished plant bug (TPB), Lygus lineolaris. Frequent use of imidacloprid is a concern for neonicotinoid resistance in this key pest. Information of how and why TPB becomes less susceptible to imidacloprid is essential for effective monitoring and managing resistance. RESULTS: Microarray analysis of 6688 genes in imidacloprid-selected TPB (Im 1500FF) revealed 955 upregulated and 1277 downregulated (>= twofold) genes in Im 1500FF, with 369 and 485 of them annotated. Five P450 and nine esterase genes were significantly upregulated, and only one esterase gene and no P450 genes were down regulated. Other upregulated genes include helicases, phosphodiesterases, ATPases and kinases. Pathway analyses identified 65 upregulated cDNAs that encode 51 different enzymes involved in 62 different pathways, including P450 and esterase genes for drug and xenobiotic metabolisms. Sixty-four downregulated cDNAs code only 17 enzymes that are associated with only 23 pathways mostly related to food digestion. CONCLUSIONS: This study demonstrated a significant change in gene expression related to metabolic processes in imidacloprid-selected TPB, resulting in overexpression of P450 and esterase genes for potential excess detoxification and cross/multiple resistance development. The identification of these and other enzyme genes establishes a foundation to explore the complicity of potential imidacloprid resistance in TPB. Published 2014. This article is a U.S. Government work and is in the public domain in the USA. C1 [Zhu, Yu Cheng; Luttrell, Randall] USDA ARS, Jamie Whitten Delta States Res Ctr, Stoneville, MS 38776 USA. RP Zhu, YC (reprint author), USDA ARS, Jamie Whitten Delta States Res Ctr, 141 Expt Stn Rd,POB 346, Stoneville, MS 38776 USA. EM yc.zhu@ars.usda.gov NR 32 TC 5 Z9 5 U1 0 U2 32 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 1526-498X EI 1526-4998 J9 PEST MANAG SCI JI Pest Manag. Sci. PD JAN PY 2015 VL 71 IS 1 BP 40 EP 57 DI 10.1002/ps.3761 PG 18 WC Agronomy; Entomology SC Agriculture; Entomology GA AW1FA UT WOS:000346034600007 PM 24515672 ER PT J AU Wang, B Lou, Z Park, B Kwon, Y Zhang, H Xu, B AF Wang, B. Lou, Z. Park, B. Kwon, Y. Zhang, H. Xu, B. TI Surface conformations of an anti-ricin aptamer and its affinity for ricin determined by atomic force microscopy and surface plasmon resonance SO PHYSICAL CHEMISTRY CHEMICAL PHYSICS LA English DT Article ID LABEL-FREE DETECTION; SINGLE-MOLECULE; DNA; THROMBIN; BINDING AB We used atomic force microscopy (AFM) and surface plasmon resonance (SPR) to study the surface conformations of an anti-ricin aptamer and its specific binding affinity for ricin molecules. The effect of surface modification of the Au(111) substrate on the aptamer affinity was also estimated. The AFM topography images had a resolution high enough to distinguish different aptamer conformations. The specific binding site on the aptamer molecule was clearly located by the AFM recognition images. The aptamer on a Au(111) surface modified with carboxymethylated-dextran (CD) showed both similarities to and differences from the one without CD modification. The influence of CD modification was evaluated using AFM images of various aptamer conformations on the Au(111) surface. The affinity between ricin and the anti-ricin aptamer was estimated using the off-rate values measured using AFM and SPR. The SPR measurements of the ricin sample were conducted in the range from 83.3 pM to 8.33 nM, and the limit of detection was estimated as 25 pM (1.5 ng mL(-1)). The off-rate values of the ricin-aptamer interactions were estimated using both single-molecule dynamic force spectroscopy (DFS) and SPR as (7.3 +/- 0.4) x 10(-4) s(-1) and (1.82 +/- 0.067) x 10(-2) s(-1), respectively. The results show that single-molecule measurements can obtain different reaction parameters from bulk solution measurements. In AFM single-molecule measurements, the various conformations of the aptamer immobilized on the gold surface determined the availability of each specific binding site to the ricin molecules. The SPR bulk solution measurements averaged the signals from specific and non-specific interactions. AFM images and DFS measurements provide more specific information on the interactions of individual aptamer and ricin molecules. C1 [Wang, B.; Xu, B.] Univ Georgia, Fac Engn, Single Mol Study Lab, Athens, GA 30602 USA. [Wang, B.; Xu, B.] Univ Georgia, Nanoscale Sci & Engn Ctr, Athens, GA 30602 USA. [Lou, Z.; Zhang, H.] Nanjing Univ Aeronaut & Astronaut, Coll Mat Sci & Technol, Nanjing 210016, Jiangsu, Peoples R China. [Park, B.] USDA ARS, Russell Res Ctr, Athens, GA 30605 USA. [Kwon, Y.] Anim & Plant Quarantine Agcy, Avian Dis Div, Anyang, South Korea. RP Xu, B (reprint author), Univ Georgia, Fac Engn, Single Mol Study Lab, Athens, GA 30602 USA. EM bxu@engr.uga.edu FU US National Science Foundation [ECCS 1231967, CBET 1139057]; Animal and Plant Quarantine Agency, Republic of Korea [I-AD15-2010-13-01]; Nature Science Foundation of Jiangsu Province, China [BK20131355] FX The authors would like to thank the partial support of this work by the US National Science Foundation (ECCS 1231967, CBET 1139057), the Animal and Plant Quarantine Agency, Republic of Korea (I-AD15-2010-13-01), and the Nature Science Foundation of Jiangsu Province, China (BK20131355). NR 30 TC 2 Z9 2 U1 7 U2 47 PU ROYAL SOC CHEMISTRY PI CAMBRIDGE PA THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND SN 1463-9076 EI 1463-9084 J9 PHYS CHEM CHEM PHYS JI Phys. Chem. Chem. Phys. PY 2015 VL 17 IS 1 BP 307 EP 314 DI 10.1039/c4cp03190c PG 8 WC Chemistry, Physical; Physics, Atomic, Molecular & Chemical SC Chemistry; Physics GA AW4FD UT WOS:000346235600032 PM 25181753 ER PT S AU Karunanithi, R Szogi, AA Bolan, N Naidu, R Loganathan, P Hunt, PG Vanotti, MB Saint, CP Ok, YS Krishnamoorthy, S AF Karunanithi, Rajasekar Szogi, Ariel A. Bolan, Nanthi Naidu, Ravi Loganathan, Paripurnanda Hunt, Patrick G. Vanotti, Matias B. Saint, Christopher P. Ok, Yong Sik Krishnamoorthy, Sathiya BE Sparks, DL TI Phosphorus Recovery and Reuse from Waste Streams SO ADVANCES IN AGRONOMY, VOL 131 SE Advances in Agronomy LA English DT Review; Book Chapter ID AEROBIC/EXTENDED-IDLE REGIME; BIOLOGICAL NUTRIENT REMOVAL; WATER-TREATMENT-PLANT; MAGNESIUM AMMONIUM PHOSPHATE; SEQUENCING BATCH REACTOR; CALCIUM-PHOSPHATE; USE-EFFICIENCY; SWINE WASTE; STRUVITE PRECIPITATION; AGRICULTURAL PHOSPHORUS AB Phosphorus (P) is a macronutrient essential for all living organisms. Regrettably, it is a finite resource since phosphate rock (PR) is the main material used for production of P fertilizers. Globally, the demand for quality PR is escalating due to many factors including increasing human population. Inevitably, the demand for PR will exceed its supply capacity. This condition will be very difficult to manage as living systems have no alternative for P. Moreover, P use efficiency is low; only 15-20% of applied P is used by crops and animals. Globally, the remaining P is shunted into various waste streams. These waste streams include large quantities of effluents rich in P from both municipal and industrial wastewater treatment systems and manure from livestock production. The P present in these waste streams poses a threat to the environment by nutrient enrichment resulting in serious ecological issues such as eutrophication of waterways. However, P in these waste streams, if economically recovered, can contribute to a sustainable management of P resources. This review covers the following aspects: global importance of P as an essential nutrient; efficient and sustainable utilization of P; waste stream production, their suitability for P recovery, and limitations; current and emerging technologies for recovery of P; and the use of recovered P material. Finally, future research needs are identified associated with P recovery from waste streams and reuse in agriculture. C1 [Karunanithi, Rajasekar; Bolan, Nanthi; Naidu, Ravi] Univ South Australia, Ctr Environm Risk Assessment & Remediat CERAR, Adelaide, SA, Australia. [Karunanithi, Rajasekar; Bolan, Nanthi; Naidu, Ravi] Univ South Australia, Cooperat Res Ctr Contaminants Assessment & Remedi, Adelaide, SA, Australia. [Szogi, Ariel A.; Hunt, Patrick G.; Vanotti, Matias B.] ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. [Loganathan, Paripurnanda] Univ Technol, Sch Civil & Environm Engn, Sydney, NSW, Australia. [Saint, Christopher P.] Univ South Australia, Sch Nat & Built Environm, Ctr Water Management & Reuse, Mawson Lakes, SA, Australia. [Ok, Yong Sik] Kangwon Natl Univ, Dept Environm Biol, Korea Biochar Res Ctr, Chunchon, South Korea. [Krishnamoorthy, Sathiya] Indian Council Agr Res, Cent Soil Water Conservat Res & Training Inst, Dehra Dun, India. RP Szogi, AA (reprint author), ARS, USDA, Coastal Plains Soil Water & Plant Res Ctr, Florence, SC 29501 USA. EM ariel.szogi@ars.usda.gov OI Saint, Christopher/0000-0002-4229-4348; Bolan, Nanthi/0000-0003-2056-1692; Ok, Yong Sik/0000-0003-3401-0912 NR 305 TC 7 Z9 7 U1 1 U2 4 PU ELSEVIER ACADEMIC PRESS INC PI SAN DIEGO PA 525 B STREET, SUITE 1900, SAN DIEGO, CA 92101-4495 USA SN 0065-2113 BN 978-0-12-802136-1 J9 ADV AGRON JI Adv. Agron. PY 2015 VL 131 BP 173 EP 250 DI 10.1016/bs.agron.2014.12.005 PG 78 WC Agronomy SC Agriculture GA BG9DY UT WOS:000393108900004 ER PT J AU Rai, DK Lawrence, P Pauszek, SJ Piccone, ME Knowles, NJ Rieder, E AF Rai, Devendra K. Lawrence, Paul Pauszek, Steve J. Piccone, Maria E. Knowles, Nick J. Rieder, Elizabeth TI Bioinformatics and Molecular Analysis of the Evolutionary Relationship between Bovine Rhinitis A Viruses and Foot-And-Mouth Disease Virus SO BIOINFORMATICS AND BIOLOGY INSIGHTS LA English DT Article DE BRAV RS3X; sequence; phylogeny; evolution; bioinformatics ID DEPENDENT RNA-POLYMERASE; RESPIRATORY SYNCYTIAL VIRUS; SWISS-MODEL WORKSPACE; ACTIVE-SITE RESIDUES; CAPSID-CODING REGION; IN-VITRO; LEADER PROTEINASE; MULTIPLE ALIGNMENTS; SECRETORY PATHWAY; SEROTYPE ASIA1 AB Bovine rhinitis viruses (BRVs) cause mild respiratory disease of cattle. In this study, a near full-length genome sequence of a virus named RS3X (formerly classified as bovine rhinovirus type 1), isolated from infected cattle from the UK in the 1960s, was obtained and analyzed. Compared to other closely related Aphthoviruses, major differences were detected in the leader protease (L-pro), P1, 2B, and 3A proteins. Phylogenetic analysis revealed that RS3X was a member of the species bovine rhinitis A virus (BRAV). Using different codon-based and branch-site selection models for Aphthoviruses, including BRAV RS3X and foot-and-mouth disease virus, we observed no clear evidence for genomic regions undergoing positive selection. However, within each of the BRV species, multiple sites under positive selection were detected. The results also suggest that the probability (determined by Recombination Detection Program) for recombination events between BRVs and other Aphthoviruses, including foot-and-mouth disease virus was not significant. In contrast, within BRVs, the probability of recombination increases. The data reported here provide genetic information to assist in the identification of diagnostic signatures and research tools for BRAV. C1 [Rai, Devendra K.; Lawrence, Paul; Pauszek, Steve J.; Piccone, Maria E.; Rieder, Elizabeth] ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. [Rai, Devendra K.] Univ Connecticut, Dept Pathobiol & Vet Sci, Storrs, CT USA. [Knowles, Nick J.] Pirbright Inst, Ash Rd, Woking, Surrey, England. RP Rieder, E (reprint author), ARS, USDA, Plum Isl Anim Dis Ctr, Greenport, NY 11944 USA. EM Elizabeth.Rieder@ars.usda.gov NR 72 TC 0 Z9 0 U1 0 U2 0 PU LIBERTAS ACAD PI AUCKLAND PA PO BOX 300-874, ALBANY 0752, AUCKLAND, 00000, NEW ZEALAND SN 1177-9322 J9 BIOINFORM BIOL INSIG JI Bioinform. Biol. Insights PY 2015 VL 9 SU 2 BP 43 EP 58 DI 10.4137/BBI.S37223 PG 16 WC Biochemical Research Methods SC Biochemistry & Molecular Biology GA EM4ND UT WOS:000395288900002 PM 27081310 ER PT J AU Kruse, A Ozer, A Johnson, R Ghanim, M Lis, J Shatters, R MacCoss, M Cilia, M AF Kruse, A. Ozer, A. Johnson, R. Ghanim, M. Lis, J. Shatters, R. MacCoss, M. Cilia, M. TI Comparative proteomics and RNA aptamer technology to identify critical factors for transmission of Candidatus Liberibacter asiacticus by the Asian citrus psyllid. SO MOLECULAR BIOLOGY OF THE CELL LA English DT Meeting Abstract C1 [Kruse, A.; Cilia, M.] Cornell Univ, Plant Pathol & Plant Microbe Biol, Ithaca, NY USA. [Kruse, A.; Cilia, M.] Boyce Thompson Inst Plant Res, Ithaca, NY USA. [Ozer, A.; Lis, J.] Cornell Univ, Mol Biol & Genet, Ithaca, NY USA. [Johnson, R.; MacCoss, M.] Univ Washington, Genome Sci, Seattle, WA 98195 USA. [Ghanim, M.] Volcani Ctr, Entomol, Bet Dagan, Israel. [Shatters, R.] US Hort Res Lab, Subtrop Insects & Hort Res Unit, Ft Pierce, Ft Pierce, FL USA. [Cilia, M.] USDA ARS, Emerging Pests & Pathogens Res Unit, Robert W Holley Ctr, Ithaca, NY 14853 USA. NR 0 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CELL BIOLOGY PI BETHESDA PA 8120 WOODMONT AVE, STE 750, BETHESDA, MD 20814-2755 USA SN 1059-1524 EI 1939-4586 J9 MOL BIOL CELL JI Mol. Biol. Cell PY 2015 VL 26 MA P766 PG 2 WC Cell Biology SC Cell Biology GA V47BX UT WOS:000209928402374 ER PT J AU Dietterich, LH Zanobetti, A Kloog, I Huybers, P Leakey, ADB Bloom, AJ Carlisle, E Fernando, N Fitzgerald, G Hasegawa, T Holbrook, NM Nelson, RL Norton, R Ottman, MJ Raboy, V Sakai, H Sartor, KA Schwartz, J Seneweera, S Usui, Y Yoshinaga, S Myers, SS AF Dietterich, Lee H. Zanobetti, Antonella Kloog, Itai Huybers, Peter Leakey, Andrew D. B. Bloom, Arnold J. Carlisle, Eli Fernando, Nimesha Fitzgerald, Glenn Hasegawa, Toshihiro Holbrook, N. Michele Nelson, Randall L. Norton, Robert Ottman, Michael J. Raboy, Victor Sakai, Hidemitsu Sartor, Karla A. Schwartz, Joel Seneweera, Saman Usui, Yasuhiro Yoshinaga, Satoshi Myers, Samuel S. TI Impacts of elevated atmospheric CO2 on nutrient content of important food crops SO SCIENTIFIC DATA LA English DT Article AB One of the many ways that climate change may affect human health is by altering the nutrient content of food crops. However, previous attempts to study the effects of increased atmospheric CO2 on crop nutrition have been limited by small sample sizes and/or artificial growing conditions. Here we present data from a metaanalysis of the nutritional contents of the edible portions of 41 cultivars of six major crop species grown using free-air CO2 enrichment ( FACE) technology to expose crops to ambient and elevated CO2 concentrations in otherwise normal field cultivation conditions. This data, collected across three continents, represents over ten times more data on the nutrient content of crops grown in FACE experiments than was previously available. We expect it to be deeply useful to future studies, such as efforts to understand the impacts of elevated atmospheric CO2 on crop macro- and micronutrient concentrations, or attempts to alleviate harmful effects of these changes for the billions of people who depend on these crops for essential nutrients. C1 [Dietterich, Lee H.] Univ Penn, Dept Biol, Philadelphia, PA 19104 USA. [Zanobetti, Antonella; Schwartz, Joel; Myers, Samuel S.] Harvard TH Chan Sch Publ Hlth, Dept Environm Hlth, Boston, MA 02215 USA. [Kloog, Itai] Ben Gurion Univ Negev, Dept Geog & Environm Dev, Beer Sheva, Israel. [Huybers, Peter] Harvard Univ, Dept Earth & Planetary Sci, Cambridge, MA 02138 USA. [Leakey, Andrew D. B.] Univ Illinois, Dept Plant Biol & Inst Genom Biol, Urbana, IL 61801 USA. [Bloom, Arnold J.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Carlisle, Eli] Univ Calif Davis, Dept Land Air & Water Resources, Davis, CA 95616 USA. [Fernando, Nimesha] Univ Melbourne, Fac Vet & Agr Sci, Melbourne, Vic 3010, Australia. [Fernando, Nimesha] Federat Univ, Ctr Environm Management, Fac Sci & Technol, Mt Helen, Vic 3350, Australia. [Fitzgerald, Glenn] Dept Econ Dev Jobs Transport & Resources, Horsham, Vic 3001, Australia. [Hasegawa, Toshihiro; Sakai, Hidemitsu; Usui, Yasuhiro] Natl Inst Agroenvironm Sci, Agrometeorol Div, Tsukuba, Ibaraki 3058604, Japan. [Holbrook, N. Michele] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA. [Nelson, Randall L.] Univ Illinois, US Dept Agr, Soybean Maize Germplasm Pathol & Genet Res Unit, Agr Res Serv,Dept Crop Sci, Urbana, IL 61801 USA. [Norton, Robert] Int Plant Nutr Inst, Horsham, Vic 3400, Australia. [Ottman, Michael J.] Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA. [Raboy, Victor] ARS, USDA, Aberdeen, ID 83210 USA. [Sartor, Karla A.] Compa Ind Inc, Los Alamos Natl Lab Environm Protect Div, Los Alamos, NM 87545 USA. [Seneweera, Saman] Univ Southern Queensland, Ctr Crop Hlth, Toowoomba, Qld 4350, Australia. [Yoshinaga, Satoshi] Natl Agr & Food Res Org, NARO Agr Res Ctr, Hokuriku Res Ctr, Niigata 9430193, Japan. [Myers, Samuel S.] Harvard Univ, Ctr Environm, Cambridge, MA 02138 USA. RP Dietterich, LH (reprint author), Univ Penn, Dept Biol, Philadelphia, PA 19104 USA. EM lee.dietterich@post.harvard.edu FU Bill & Melinda Gates Foundation; Winslow Foundation; Commonwealth Department of Agriculture (Australia); International Plant Nutrition Institute, (Australia); Grains Research and Development Corporation (Australia); Ministry of Agriculture, Forestry and Fisheries (Japan); National Science Foundation (NSF) [IOS-13-58675]; USDA NIFA [2008-35100-044459]; US Department of Agriculture, Agricultural Research Service; Illinois Council for Food and Agricultural Research (CFAR); Department of Energy's Office of Science (BER) Midwestern Regional Center of the National Institute for Climatic Change Research at Michigan Technological University [DEFC02- 06ER64158]; National Research Initiative of Agriculture and Food Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [2010-65114-20343]; Harvard Catalyst \ The Harvard Clinical and Translational Science Center (National Center for Research Resources); Harvard Catalyst \ The Harvard Clinical and Translational Science Center (National Center for Advancing Translational Sciences, National Institutes of Health) [8UL1TR000170-05] FX We thank H. Nakamura, T. Tokida, and C. Zhu for contributions to the RiceFACE project. We thank the following for financial support of this work: the Bill & Melinda Gates Foundation; the Winslow Foundation; the Commonwealth Department of Agriculture (Australia), the International Plant Nutrition Institute, (Australia), the Grains Research and Development Corporation (Australia), the Ministry of Agriculture, Forestry and Fisheries (Japan); the National Science Foundation (NSF IOS-13-58675); USDA NIFA 2008-35100-044459; research at SoyFACE was supported by the US Department of Agriculture, Agricultural Research Service, which also provided support via the Small Grains and Potato Research Unit, Aberdeen ID; Illinois Council for Food and Agricultural Research (CFAR); Department of Energy's Office of Science (BER) Midwestern Regional Center of the National Institute for Climatic Change Research at Michigan Technological University, under Award Number DEFC02- 06ER64158; and the National Research Initiative of Agriculture and Food Research Initiative Competitive Grants Program Grant no. 2010-65114-20343 from the USDA National Institute of Food and Agriculture. Early stages of this work received support from Harvard Catalyst vertical bar The Harvard Clinical and Translational Science Center (National Center for Research Resources and the National Center for Advancing Translational Sciences, National Institutes of Health Award 8UL1TR000170-05). Samuel S. Myers had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. NR 42 TC 1 Z9 1 U1 5 U2 16 PU NATURE PUBLISHING GROUP PI LONDON PA MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND SN 2052-4463 J9 SCI DATA JI Sci. Data PY 2015 VL 2 AR 150036 DI 10.1038/sdata.2015.36 PG 8 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA V45VM UT WOS:000209844100033 PM 26217490 ER PT J AU Wacker, JP Brashaw, BK Jalinoos, F AF Wacker, J. P. Brashaw, B. K. Jalinoos, F. BE Ingraffea, N Libby, M TI A Field Assessment of Timber Highway Bridge Durability in the United States SO Structures Congress 2015 LA English DT Proceedings Paper CT Structures Congress CY APR 23-25, 2015 CL Portland, OR SP Amer Soc Civil Engineers, Struct Engn Inst AB This paper summarizes a cooperative project to assess the current condition and life expectancy of 132 timber highway bridge superstructures at locations throughout the United States. Several superstructure types were included in this comprehensive effort, of which two-thirds were sawn timber stringer systems. In-depth inspections were conducted by the project team using visual, probing, and nondestructive evaluation (NDE) techniques to characterize the condition of the primary bridge superstructure components. The condition of the bridges was satisfactory and better for large percentages of the superstructure subsets, even within those regions of the country that have higher hazard ratings for exposed wood structures. Inspection results show that timber is a viable option for primary structural members in highway bridges with satisfactory service-life estimation of 70 years or more. These results shall provide the basis for the development of life-cycle cost analyses and bridge deterioration rate modeling for timber bridge superstructures in the future. C1 [Wacker, J. P.] USDA, ASCE, Forest Prod Lab, Forest Serv, One Gifford Pinchot Dr, Madison, WI 53726 USA. [Brashaw, B. K.] Univ Minnesota, Nat Resources Res Inst, Duluth, MN 55811 USA. [Jalinoos, F.] US Dept Transportat, Turner Fairbank Highway Res Ctr, Fed Highway Adm, Mclean, VA 22101 USA. RP Wacker, JP (reprint author), USDA, ASCE, Forest Prod Lab, Forest Serv, One Gifford Pinchot Dr, Madison, WI 53726 USA. EM jwacker@fs.fed.us; bbrashaw@nrri.umn.edu; frank.jalinoos@dot.gov NR 7 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CIVIL ENGINEERS PI NEW YORK PA UNITED ENGINEERING CENTER, 345 E 47TH ST, NEW YORK, NY 10017-2398 USA BN 978-0-7844-7911-7 PY 2015 BP 278 EP 286 PG 9 WC Construction & Building Technology; Engineering, Civil; Engineering, Mechanical SC Construction & Building Technology; Engineering GA BG8WG UT WOS:000392737000024 ER PT J AU Cunningham, K Lattanzi, D Andrea, RD Riley, M Huette, T Goetz, R Wilson, R AF Cunningham, K. Lattanzi, D. Andrea, R. Dell' Riley, M. Huette, T. Goetz, R. Wilson, R. BE Ingraffea, N Libby, M TI UAS-Based Inspection of the Placer River Trail Bridge: A Data-Driven Approach SO Structures Congress 2015 LA English DT Proceedings Paper CT Structures Congress CY APR 23-25, 2015 CL Portland, OR SP Amer Soc Civil Engineers, Struct Engn Inst AB Over the last decade, unmanned aerial systems (UAS) technology has matured to the point that it is now a viable option for augmenting more traditional bridge inspection methods. Any UAS-based inspection must address questions regarding the design of the aerial platform, the choice of on-board inspection cameras, and the protocols for the human pilot to follow. The wide variety of currently available UASs and imaging systems, as well as a current lack of UAS inspection standards, can pose challenges to engineers attempting to design or test a new system. The Placer River Trail Bridge, located on the Alaskan Kenai Peninsula, has been chosen as a test site for the prototyping and testing of a new UAS bridge inspection system. The desired final inspection product guided the design and operation of the UAS. The research team emphasized a data-driven approach in developing the UAS and its associated inspection protocols. Goals included providing high-resolution scans of the bridge capable of detecting a variety of damage indicators, and producing a 3D virtual model of the bridge using Structure-from-Motion. The resulting designs of both the UAS and the inspection protocols are presented herein. The results indicate that such a data-driven approach can lead to UAS inspection systems that are more capable of meeting the needs of bridge asset managers and inspectors. C1 [Cunningham, K.; Wilson, R.] Univ Alaska Fairbanks, Scenarios Network Alaska & Arctic Planning, POB 5245, Fairbanks, AK 99775 USA. [Lattanzi, D.] George Mason Univ, Fairfax, VA 22030 USA. [Andrea, R. Dell'] US Forest Serv, Juneau, AK 99801 USA. [Riley, M.] US Forest Serv, Juneau, AK 99801 USA. [Huette, T.; Goetz, R.] US Forest Serv, Juneau, AK 99802 USA. RP Cunningham, K (reprint author), Univ Alaska Fairbanks, Scenarios Network Alaska & Arctic Planning, POB 5245, Fairbanks, AK 99775 USA. EM kwcunningham@alaska.edu; dlattanz@gmu.edu; rdellandrea@fs.fed.us; markriley@fs.fed.us; thuette@fs.fed.us; rgoetz@fs.fed.us; rayjan.wilson@alaska.edu OI Lattanzi, David/0000-0001-9247-0680 NR 3 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CIVIL ENGINEERS PI NEW YORK PA UNITED ENGINEERING CENTER, 345 E 47TH ST, NEW YORK, NY 10017-2398 USA BN 978-0-7844-7911-7 PY 2015 BP 607 EP 615 PG 9 WC Construction & Building Technology; Engineering, Civil; Engineering, Mechanical SC Construction & Building Technology; Engineering GA BG8WG UT WOS:000392737000053 ER PT J AU Shirmeen, T Jia, YF Locke, MA Lizotte, RE AF Shirmeen, Tahmina Jia, Yafei Locke, Martin A. Lizotte, Richard E., Jr. BE Karvazy, K Webster, VL TI Numerical Modeling of Rain induced Overland Flows SO World Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems LA English DT Proceedings Paper CT World Environmental and Water Resources Congress CY MAY 17-21, 2015 CL Austin, TX SP Amer Soc Civil Engineers, Environm and Water Resources Inst DE overland flow; rainfall-runoff; numerical modeling; simulation; kinematic wave model ID PLANE AB Runoff is a basic hydrologic process affecting management activities in agricultural watersheds. Better description of runoff patterns through modeling will help to understand and predict watershed sediment transport and water quality. In this study, CCHE2D, a numerical model for general free surface flow hydrodynamics, is applied to study the runoff and channel flow mixed problems. The model's capability of simulating the rainfall generated runoff processes is tested using analytical solutions, experimental data and field data. Comparison of numerical solutions with both analytic solutions and observed overland flows resulting from unsteady rainfalls and complex topography is satisfactory. The model is applied to a real world agricultural watershed in northwestern Mississippi, USA, in the Mississippi River Alluvial Plain known as the Mississippi Delta. The fine resolution of the numerical simulations resolved detailed runoff patterns in watersheds which can be used for soil erosion and gully development analyses. C1 [Shirmeen, Tahmina; Jia, Yafei] Univ Mississippi, NCCHE, University, MS 38677 USA. [Locke, Martin A.; Lizotte, Richard E., Jr.] USDA ARS, Natl Sedimentat Lab, Water Qual & Ecol Res Unit, Oxford, MS 38655 USA. RP Shirmeen, T (reprint author), Univ Mississippi, NCCHE, University, MS 38677 USA. EM tshirmeen@ncche.olemiss.edu; jia@ncche.olemiss.edu; martin.locke@ars.usda.gov; richard.lizotte@ars.usda.gov FU US Department of Homeland Security via the Southeast Region Research Initiative (SERRI) project; USDA Agriculture Research Service [58-6408-1-609] FX This work is supported in part by US Department of Homeland Security via the Southeast Region Research Initiative (SERRI) project and USDA Agriculture Research Service under the Specific Research Agreement No. 58-6408-1-609 monitored by the USDA-ARS National Sedimentation Laboratory (NSL) and The University of Mississippi (UM). NR 19 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CIVIL ENGINEERS PI NEW YORK PA UNITED ENGINEERING CENTER, 345 E 47TH ST, NEW YORK, NY 10017-2398 USA BN 978-0-7844-7916-2 PY 2015 BP 1532 EP 1544 PG 13 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA BG8ZE UT WOS:000392856300150 ER PT J AU Gallisdorfer, MS Ghaneeizad, SM Bennett, SJ Atkinson, JF Cai, D Simon, A Langendoen, EJ AF Gallisdorfer, M. S. Ghaneeizad, S. M. Bennett, S. J. Atkinson, J. F. Cai, D. Simon, A. Langendoen, E. J. BE Karvazy, K Webster, VL TI Responses of experimental river corridors to engineered log jams SO World Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems LA English DT Proceedings Paper CT World Environmental and Water Resources Congress CY MAY 17-21, 2015 CL Austin, TX SP Amer Soc Civil Engineers, Environm and Water Resources Inst ID LARGE WOODY DEBRIS; CHANNELS; STREAMS AB Physical models of the Big Sioux River, SD, were constructed to assess the impact on flow, drag, and bed erosion and deposition in response to the installation of two different types of engineered log jams (ELJs). A fixed-bed model focused on flow velocity and forces acting on an instrumented ELJ, and a movable-bed model focused on channel morphodynamics. The results show that: (1) downstream flow velocity, as expected, can be markedly affected by the presence of the ELJs, (2) both ELJ types afforded relatively large regions of bank protection, and (3) the relatively larger ELJ had a higher drag coefficient, relatively larger areas of bed deformation, and greater impact on the opposite stream bank as compared to the relatively smaller ELJ. These modeling results show that the style and size of ELJs can have a significant impact on flow resistance, bank protection, and stream channel responses, which should facilitate practitioners in the effective design of ELJs for natural rivers. C1 [Gallisdorfer, M. S.; Bennett, S. J.; Cai, D.] Univ Buffalo, Dept Geog, Buffalo, NY 14261 USA. [Ghaneeizad, S. M.; Atkinson, J. F.] Univ Buffalo, Dept Civil Struct & Environm Engn, Buffalo, NY 14260 USA. [Simon, A.] CardnoENTRIX, Oxford, MS 38655 USA. [Langendoen, E. J.] USDA ARS, Natl Sedimentat Lab, Oxford, MS 38655 USA. RP Gallisdorfer, MS (reprint author), Univ Buffalo, Dept Geog, Buffalo, NY 14261 USA. EM msg5@buffalo.edu; sghaneei@buffalo.edu; seanb@buffalo.edu; atkinson@buffalo.edu; donghuac@buffalo.edu; andrew.simon@cardno.com; eddy.langendoen@ars.usda.gov OI Langendoen, Eddy/0000-0002-2215-4989 NR 17 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CIVIL ENGINEERS PI NEW YORK PA UNITED ENGINEERING CENTER, 345 E 47TH ST, NEW YORK, NY 10017-2398 USA BN 978-0-7844-7916-2 PY 2015 BP 1811 EP 1820 PG 10 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA BG8ZE UT WOS:000392856300177 ER PT J AU Taghvaeian, S Paul, G Ochsner, TE Gowda, P Marek, T AF Taghvaeian, S. Paul, G. Ochsner, T. E. Gowda, P. Marek, T. BE Karvazy, K Webster, VL TI A Comparison between Evapotranspiration Estimates based on Remotely Sensed Surface Energy Balance and Ground-based Soil Water Balance Analyses SO World Environmental and Water Resources Congress 2015: Floods, Droughts, and Ecosystems LA English DT Proceedings Paper CT World Environmental and Water Resources Congress CY MAY 17-21, 2015 CL Austin, TX SP Amer Soc Civil Engineers, Environm and Water Resources Inst ID MAPPING EVAPOTRANSPIRATION; OKLAHOMA MESONET; MODEL; TEMPERATURE; MANAGEMENT; ALGORITHM; MOISTURE; NETWORK; IMAGERY AB Remotely sensed and in-situ data were used to investigate dynamics of root zone soil moisture and evapotranspiration (ET) at four Mesonet stations in north-central Oklahoma over an 11-year period (2000-2010). Two moisture deficit indicators based on soil matric potential had spatial and temporal patterns that were consistent with collected precipitation and reference ET data, as well as the state-wide historical trends. Actual ET was estimated by closing the soil water balance using in-situ soil moisture data, as well as by applying a surface energy balance approach to Landsat Thematic Mapper 5 imagery. When averaged over all years, weekly ET estimates with both approaches had a small mean signed difference (0.5 mm.week(-1)), but a large root mean square deviation (11.6 mm.week(-1)). This difference may be partly due to errors caused by interpolation of ET estimates between less-frequent satellite overpasses. Averaging over longer periods or modifying the interpolation methods may improve the accuracies for short-term remotely sensed ET estimates. C1 [Taghvaeian, S.] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. [Paul, G.; Marek, T.] Texas A&M AgriLife Res, Amarillo, TX USA. [Ochsner, T. E.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA. [Gowda, P.] USDA ARS, Conservat & Prod Res Lab, PO Drawer 10, Bushland, TX 79012 USA. RP Taghvaeian, S (reprint author), Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74078 USA. EM Saleh.Taghvaeian@okstate.edu FU USDA through the National Institute for Food and Agriculture's Agriculture [2012-02355]; Food Research Initiative, Regional Approaches for Adaptation to and Mitigation of Climate Variability and Change FX Partial funding was provided by USDA Project No. 2012-02355 through the National Institute for Food and Agriculture's Agriculture and Food Research Initiative, Regional Approaches for Adaptation to and Mitigation of Climate Variability and Change. NR 23 TC 0 Z9 0 U1 0 U2 0 PU AMER SOC CIVIL ENGINEERS PI NEW YORK PA UNITED ENGINEERING CENTER, 345 E 47TH ST, NEW YORK, NY 10017-2398 USA BN 978-0-7844-7916-2 PY 2015 BP 1971 EP 1980 PG 10 WC Engineering, Civil; Environmental Sciences; Water Resources SC Engineering; Environmental Sciences & Ecology; Water Resources GA BG8ZE UT WOS:000392856300193 ER PT J AU Saha, BC Nichols, NN Qureshi, N Kennedy, GJ Iten, LB Cotta, MA AF Saha, Badal C. Nichols, Nancy N. Qureshi, Nasib Kennedy, Gregory J. Iten, Loren B. Cotta, Michael A. TI Pilot scale conversion of wheat straw to ethanol via simultaneous saccharification and fermentation SO BIORESOURCE TECHNOLOGY LA English DT Article DE Ethanol; Wheat straw; Simultaneous saccharification and fermentation; Recombinant ethanologenic Escherichia coli; Ethanol from wheat straw ID RECOMBINANT ESCHERICHIA-COLI; DILUTE-ACID PRETREATMENT; SACCHAROMYCES-CEREVISIAE; ENZYMATIC SACCHARIFICATION; LIGNOCELLULOSIC BIOMASS; CORN STOVER; XYLOSE; FRACTIONATION; HYDROLYSIS; GLUCOSE AB The production of ethanol from wheat straw (WS) by dilute acid pretreatment, bioabatement of fermentation inhibitors by a fungal strain, and simultaneous saccharification and fermentation (SSF) of the bio-abated WS to ethanol using an ethanologenic recombinant bacterium was studied at a pilot scale without sterilization. WS (124.2 g/L) was pretreated with dilute H2SO4 in two parallel tube reactors at 160 degrees C. The inhibitors were bio-abated by growing the fungus aerobically. The maximum ethanol produced by SSF of the bio-abated WS by the recombinant Escherichia coli FBR5 at pH 6.0 and 35 degrees C was 36.0 g/L in 83 h with a productivity of 0.43 g L-1 h(-1). This value corresponds to an ethanol yield of 0.29 g/g of WS which is 86% of the theoretical ethanol yield from WS. This is the first report on the production of ethanol by the recombinant bacterium from a lignocellulosic biomass at a pilot scale. Published by Elsevier Ltd. C1 [Saha, Badal C.; Nichols, Nancy N.; Qureshi, Nasib; Kennedy, Gregory J.; Iten, Loren B.; Cotta, Michael A.] USDA ARS, Natl Ctr Agr Utilizat Res, Bioenergy Res Unit, Peoria, IL 61604 USA. RP Saha, BC (reprint author), USDA ARS, Natl Ctr Agr Utilizat Res, 1815 N Univ St, Peoria, IL 61604 USA. EM Badal.Saha@ars.usda.gov NR 28 TC 14 Z9 16 U1 2 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JAN PY 2015 VL 175 BP 17 EP 22 DI 10.1016/j.biortech.2014.10.060 PG 6 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AU6CJ UT WOS:000345689900003 PM 25459799 ER PT J AU Weimer, PJ Nerdahl, M Brandl, DJ AF Weimer, Paul J. Nerdahl, Michael Brandl, Dane J. TI Production of medium-chain volatile fatty acids by mixed ruminal microorganisms is enhanced by ethanol in co-culture with Clostridium kluyveri SO BIORESOURCE TECHNOLOGY LA English DT Article DE Caproic acid; Carboxylate platform; Clostridium kluyveri; Ethanol; Rumen ID HEXANOIC ACID; BOVINE RUMEN; CAPROIC ACID; FERMENTATION; CELLULOSE; PLATFORM; CULTURE; BIOMASS; FUELS AB Mixed bacterial communities from the rumen ferment cellulosic biomass primarily to C-2-C-4 volatile fatty acids, and perform only limited chain extension to produce C-5 (valeric) and C-6 (caproic) acids. The aim of this study was to increase production of caproate and valerate in short-term in vitro incubations. Coculture of mixed ruminal microbes with a rumen-derived strain of the bacterium Clostridium kluyveri converted cellulosic biomass (alfalfa stems or switchgrass herbage) plus ethanol to VFA mixtures that include valeric and caproic acids as the major fermentation products over a 48-72 h run time. Concentrations of caproate reached 6.1 g L-1, similar to or greater than those reported in most conventional carboxylate fermentations that employ substantially longer run times. Published by Elsevier Ltd. C1 [Weimer, Paul J.] USDA ARS, USDFRC, Madison, WI 53706 USA. [Weimer, Paul J.; Nerdahl, Michael; Brandl, Dane J.] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. RP Weimer, PJ (reprint author), USDA ARS, USDFRC, 1925 Linden Dr West, Madison, WI 53717 USA. EM Paul.Weimer@ars.usda.gov FU USDA Agricultural Research Service through CRIS [3655-41000-06-00D, 3655-31000-24-00D] FX We thank C.L. Odt for HPLC analysis and K.J. Shinners for biomass samples. This research was supported by the USDA Agricultural Research Service through CRIS projects 3655-41000-06-00D and 3655-31000-24-00D. NR 25 TC 9 Z9 9 U1 6 U2 61 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0960-8524 EI 1873-2976 J9 BIORESOURCE TECHNOL JI Bioresour. Technol. PD JAN PY 2015 VL 175 BP 97 EP 101 DI 10.1016/j.biortech.2014.10.054 PG 5 WC Agricultural Engineering; Biotechnology & Applied Microbiology; Energy & Fuels SC Agriculture; Biotechnology & Applied Microbiology; Energy & Fuels GA AU6CJ UT WOS:000345689900013 PM 25459809 ER PT J AU Manor, ML Cleveland, BM Weber, GM Kenney, PB AF Manor, Meghan L. Cleveland, Beth M. Weber, Gregory M. Kenney, P. Brett TI Effects of sexual maturation and feeding level on fatty acid metabolism gene expression in muscle, liver, and visceral adipose tissue of diploid and triploid rainbow trout, Oncorhynchus mykiss SO COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY LA English DT Article DE triploid; salmonid; multiplex-GeXP; Fatty acid; Metabolism ID REGULATES LIPID-METABOLISM; PROXIMATE COMPOSITION; MAMMALIAN TARGET; SKELETAL-MUSCLE; SALMO-GAIRDNERI; DIFFERENT DIETS; ENERGY STORES; IN-VIVO; GROWTH; INSULIN AB In many cultured fish species, such as salmonids, gonadal development occurs at the expense of stored energy and nutrients, including lipids. However, mechanisms regulating nutrient repartitioning during sexual maturation are not well understood. This study compared sexually maturing diploid (2 N) and sterile triploid (3 N) female rainbow trout to investigate effects of sexual maturation on expression of 35 genes involved in fatty acid metabolism, including genes within fatty acid synthesis, beta-oxidation, and cofactors of the mTOR and PPAR signaling pathways, in liver, white muscle, and visceral adipose tissue. Diploid fish were fed at different rations (0.25% and 0.50% tank biomass, and satiation) to determine effects of ration on gene expression. Gene expression was affected by ration level only in white muscle; erk and acat2 had higher expression in fish fed higher rations. On the other hand, sexual maturation affected gene expression across all three tissue types. Data indicate 2 N fish have higher expression of beta-oxidation genes within white muscle and within visceral adipose tissue. These findings support enhanced fatty acid mobilization within these tissues during sexual maturation. Higher expression of fatty acid synthesis genes in 3 N female liver is associated with higher expression of mTOR cofactors and ppar gamma, which reflects continued deposition of lipids in these fish. Furthermore, greater expression of genes involved in beta-oxidation pathways across ration levels in 2 N females suggests that sexual maturation and the associated maturation-related signals are stronger regulators of lipid metabolism-related genes rather the rations applied in the current study. (C) 2014 Elsevier Inc. All rights reserved. C1 [Manor, Meghan L.; Kenney, P. Brett] W Virginia Univ, Div Anim & Nutr Sci, Davis Coll Agr Nat Resources & Design, Morgantown, WV 26506 USA. [Cleveland, Beth M.; Weber, Gregory M.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Manor, ML (reprint author), W Virginia Univ, Div Anim & Nutr Sci, 1042 Agr Sci Bldg POB 6108, Morgantown, WV 26505 USA. EM mmanor@mix.wvu.edu FU USDA/CSREES [2007-35205-17914]; USDA-ARS [58-1930-0-059]; National Research Initiative Competitive Grants Program; USDA/ARS CRIS [1930-31000-010-000D] FX This study was funded by USDA/CSREES #2007-35205-17914 USDA-ARS Cooperative Agreement No. 58-1930-0-059 National Research Initiative Competitive Grants Program and supported by USDA/ARS CRIS Project 1930-31000-010-000D and USDA-ARS Cooperative Agreement No. 58-1930-0-059. It is published with the approval of the West Virginia University Director of the Agricultural Station as scientific paper XXXX. NR 43 TC 3 Z9 3 U1 4 U2 37 PU ELSEVIER SCIENCE INC PI NEW YORK PA 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA SN 1096-4959 EI 1879-1107 J9 COMP BIOCHEM PHYS B JI Comp. Biochem. Physiol. B-Biochem. Mol. Biol. PD JAN PY 2015 VL 179 BP 17 EP 26 DI 10.1016/j.cbpb.2014.09.003 PG 10 WC Biochemistry & Molecular Biology; Zoology SC Biochemistry & Molecular Biology; Zoology GA AU9AG UT WOS:000345883700003 PM 25242626 ER PT J AU Yi, J Lam, TI Yokoyama, W Cheng, LW Zhong, F AF Yi, Jiang Lam, Tina I. Yokoyama, Wallace Cheng, Luisa W. Zhong, Fang TI Beta-carotene encapsulated in food protein nanoparticles reduces peroxyl radical oxidation in Caco-2 cells SO FOOD HYDROCOLLOIDS LA English DT Article DE Beta-carotene; Protein; Nano-particles; Antioxidant activity; Caco-2 cells ID ANTIOXIDANT ACTIVITY; EVAPORATION METHOD; ALPHA-TOCOPHEROL; STABILITY; EMULSIONS; DIGESTION; BIOACCESSIBILITY; NANOEMULSIONS; NANODISPERSIONS; BIOAVAILABILITY AB Beta-carotene (BC) was encapsulated by sodium caseinate (SC), whey protein isolate (WPI), and soybean protein isolate (SPI) by the homogenization-evaporation method forming nanoparticles of 78, 90 and 370 nm diameter. Indices of the chemical antioxidant assays, the reducing power, DPPH radical scavenging activity, and the hydroxyl radical scavenging activity (OH center dot) were improved by nanoencapsulation, compared to free BC. Caco-2 cells were treated with dichlorofluorescin diacetate (DCFH-DA) to determine the cellular antioxidant activity (CAA) of BC. CAA of SC, WPI, and SPI nanoparticles was higher (60%) compared to BC alone (45%). FTIR-ATR showed the presence of BC in the nanoparticle preparations. XRD and DSC analyses suggested that BC is amorphous in the nanoparticles. BC encapsulated by WPI had the most advantageous release properties. Release was low with pepsin but high with trypsin suggesting that WPI might be the best protein delivery vehicle to deliver BC to the intestine. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Yi, Jiang; Zhong, Fang] Jiangnan Univ, Sch Food Sci & Technol, Key Lab Food Colloids & Biotechnol, Minist Educ, Wuxi 214122, Peoples R China. [Yi, Jiang; Lam, Tina I.; Yokoyama, Wallace; Cheng, Luisa W.] USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA. RP Zhong, F (reprint author), Jiangnan Univ, Sch Food Sci & Technol, Key Lab Food Colloids & Biotechnol, Minist Educ, Wuxi 214122, Peoples R China. EM fzhong@jiangnan.edu.cn FU National 125 Program [2011BAD23B02, 2013AA1022207]; NSFC [31171686]; 111 Project [B07029]; [NSF-Jiangsu-BK2012556]; [PCSIRT0627]; [JUSRP11422] FX This work was financially supported by National 125 Program 2011BAD23B02, 2013AA1022207; NSFC 31171686; NSF-Jiangsu-BK2012556; 111 Project B07029; PCSIRT0627 and JUSRP11422. NR 38 TC 21 Z9 22 U1 18 U2 84 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD JAN PY 2015 VL 43 BP 31 EP 40 DI 10.1016/j.foodhyd.2014.04.028 PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AU6AD UT WOS:000345683500005 ER PT J AU Pan, Y Tikekar, RV Wang, MS Avena-Bustillos, RJ Nitin, N AF Pan, Yuanjie Tikekar, Rohan V. Wang, Min S. Avena-Bustillos, Roberto J. Nitin, Nitin TI Effect of barrier properties of zein colloidal particles and oil-in-water emulsions on oxidative stability of encapsulated bioactive compounds SO FOOD HYDROCOLLOIDS LA English DT Article DE Free radical induced oxidation; Oxygen; Zein colloidal particle; Oil-in-water emulsion; Fluorescence spectroscopy ID CONTROLLED-RELEASE; DELIVERY-SYSTEMS; TRANS-RETINOL; FREE-RADICALS; VITAMIN-A; NANOPARTICLES; ANTIOXIDANT; BIOAVAILABILITY; CURCUMIN; PROTEIN AB Background: Oxidation of encapsulated bioactive compounds is a key challenge that limits shelf-life of bioactive containing products. The objectives of this study were to compare differences between the oxidative barrier properties of biopolymer particle based encapsulation system (zein colloidal particles) and oil-in-water emulsions and evaluate the impact of these differences on oxidative stability of encapsulated bioactives. Methods: Both zein colloidal particles and oil-in-water emulsions were stabilized by casein protein. The oxidative barrier properties of the selected encapsulation systems were determined by measuring the permeation rate of peroxyl radicals and oxygen across the interface. Peroxyl radical permeation rates were correlated with stability of a model bioactive, curcumin and oxygen permeation rates were correlated with stability of another model bioactive, retinol. Results: Radical permeation rate was significantly higher in oil-in-water emulsions compared to zein colloidal particles, indicating enhanced barrier property of zein colloidal particles against peroxyl radical induced oxidation. Consistent with these results, stability of curcumin encapsulated in zein colloidal particles was significantly higher compared to that in oil-in-water emulsions. Oxygen permeation measurements showed no significant differences in the barrier properties of both encapsulation systems against oxygen permeation. Consistent with these results, the oxidative stability of retinol was similar in both encapsulation systems. Conclusions: The results of this study demonstrate the advantages of biopolymer particle based encapsulation system in limiting free radical induced oxidation of encapsulated bioactives and also demonstrate the ineffectiveness of both encapsulation systems in limiting oxygen permeation. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Pan, Yuanjie; Wang, Min S.; Nitin, Nitin] Univ Calif Davis, Dept Food Sci & Technol, Davis, CA 95616 USA. [Tikekar, Rohan V.] Drexel Univ, Sch Technol & Profess Studies, Program Food Sci, Philadelphia, PA 19104 USA. [Avena-Bustillos, Roberto J.] USDA, Western Reg Res Ctr, Agr Res Serv, Albany, CA 94710 USA. [Nitin, Nitin] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA. RP Nitin, N (reprint author), 2221 RMI South,Old Davis Rd, Davis, CA 95616 USA. EM nnitin@ucdavis.edu OI Tikekar, Rohan/0000-0002-7915-3848 NR 34 TC 8 Z9 8 U1 5 U2 61 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD JAN PY 2015 VL 43 BP 82 EP 90 DI 10.1016/j.foodhyd.2014.05.002 PG 9 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AU6AD UT WOS:000345683500011 ER PT J AU Zhang, F Luan, T Kang, D Jin, QW Zhang, HB Yadav, MP AF Zhang, Fei Luan, Tu Kang, Ding Jin, Qiangwei Zhang, Hongbin Yadav, Madhav P. TI Viscosifying properties of corn fiber gum with various polysaccharides SO FOOD HYDROCOLLOIDS LA English DT Article DE Corn fiber gum; Viscosity; Shear stress synergism index; Rheology; Polysaccharides; Flow behavior ID XANTHAN GUM; EMULSIFYING PROPERTIES; RHEOLOGICAL BEHAVIOR; VISCOUS SYNERGISM; MIXED-SOLUTIONS; PHASE-BEHAVIOR; MIXTURES; WATER; SHEAR; GELS AB The effect of corn fiber gum (CFG) on the aqueous solutions of a series of widely-used commercial polysaccharides has been studied by rheological techniques using a shear stress synergism index I-S to evaluate its viscosifying action. While CFG solution exhibited Newtonian fluid behavior with a very low viscosity even at a high concentration, the aqueous mixtures of CFG and some non-gelling polysaccharides (hyaluronan, guar gum, carboxymethylcellulose, hydroxyethylcellulose, konjac glucomannan, pectin and chitosan) showed a pseudoplastic fluid behavior. Furthermore, the addition of CFG showed a remarkable viscosifying action for the aqueous solutions of these non-gelling polysaccharides likely due to their interaction by hydrogen bonding. However, the viscosity behavior of CFG with some gelling polysaccharides such as methylcellulose, gellan gum, carrageenan, xanthan, may be based on a different mechanism. A proper model of intermolecular interaction between CFG and these gelling and non-gelling polysaccharides has been discussed and proposed. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Zhang, Fei; Luan, Tu; Kang, Ding; Jin, Qiangwei; Zhang, Hongbin] Shanghai Jiao Tong Univ, Sch Chem & Chem Technol, Adv Rheol Inst, Dept Polymer Sci & Engn, Shanghai 200240, Peoples R China. [Yadav, Madhav P.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Zhang, HB (reprint author), Shanghai Jiao Tong Univ, Sch Chem & Chem Technol, Adv Rheol Inst, Dept Polymer Sci & Engn, Shanghai 200240, Peoples R China. EM hbzhang@sjtu.edu.cn FU National Natural Science Foundation of China [21274090, 21074071]; Shanghai Leading Academic Discipline Project [B202] FX The authors are thankful to the National Natural Science Foundation of China (Grant No. 21274090, 21074071) and the Shanghai Leading Academic Discipline Project (No. B202) for the financial support to accomplish this work. Dr. F. Yu is acknowledged for the preparation of polyamidoamine dendrimers. The authors are also thankful to Stefanie Simon for her technical assistance in preparing very pure CFG. NR 52 TC 5 Z9 6 U1 2 U2 30 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0268-005X EI 1873-7137 J9 FOOD HYDROCOLLOID JI Food Hydrocolloids PD JAN PY 2015 VL 43 BP 218 EP 227 DI 10.1016/j.foodhyd.2014.05.018 PG 10 WC Chemistry, Applied; Food Science & Technology SC Chemistry; Food Science & Technology GA AU6AD UT WOS:000345683500027 ER PT J AU Tian, GL Chiu, CY Franzluebbers, AJ Oladeji, OO Granato, TC Cox, AE AF Tian, Guanglong Chiu, Chih-Yu Franzluebbers, Alan J. Oladeji, Olawale O. Granato, Thomas C. Cox, Albert E. TI Biosolids amendment dramatically increases sequestration of crop residue-carbon in agricultural soils in western Illinois SO APPLIED SOIL ECOLOGY LA English DT Article DE Carbon sink; Microbial metabolic quotient; Microbial stress; Midwest ID LONG-TERM APPLICATION; ORGANIC-MATTER; MICROBIAL BIOMASS; NITROGEN-FERTILIZATION; LAND RECLAMATION; NMR-SPECTROSCOPY; C-13 ABUNDANCE; DECOMPOSITION; DYNAMICS; ECOSYSTEM AB In agricultural soils, a large portion of C in crop residues (i.e., non-harvested plant parts left in the field) is annually lost to atmosphere due to the low C use metabolism of soil microorganisms adapting to the environmental stress (moisture stress and substrate C and N imbalance). In this study, we tested the hypothesis that amending soil with biosolids (treated sewage sludge with high stable organic matter and low C:N ratio) can improve the C metabolism of microorganisms in agricultural soils through alleviation of microbial stress, leading to increased sequestration of crop residue-C in agricultural soils. Biosolids were applied at a mean annual rate of 4.2 kg m (2) (dry weight) to eight agricultural fields (biosolids-amended) for 13 years (1972-1984) in western Illinois. Four agricultural fields (unamended) received chemical fertilizer as control. We measured the sequestration rate of crop residue-C in the soils over the span of 34 years (1972-2006) using a C-13 technique. We found dramatically greater sequestration rate of crop residue-C in biosolids-amended soil (32.5 +/- 1.7% of total crop residue-C) versus unamended soil (11.8+1.6%). Soil microbial metabolic quotient was significantly lower in biosolids-amended than in unamended fields, indicating that biosolid-amendment reduced soil microbial stress and improved microbial C metabolism. The study concludes use of a soil amendment with high stable C and low C:N is a valid approach to transform agricultural soils from current C-neutral status to a C sink. Biosolids represent a good choice of such soil amendments. (C) 2014 Elsevier B.V. All rights reserved. C1 [Tian, Guanglong; Oladeji, Olawale O.; Granato, Thomas C.; Cox, Albert E.] Metropolitan Water Reclamat Dist Greater Chicago, Monitoring & Res Dept, Environm Monitoring & Res Div, Cicero, IL 60804 USA. [Tian, Guanglong] IIT, Dept Civil Architectural & Environm Engn, Chicago, IL 60616 USA. [Chiu, Chih-Yu] Acad Sinica, Biodivers Res Ctr, Taipei 11529, Taiwan. [Franzluebbers, Alan J.] USDA ARS, Raleigh, NC 27695 USA. RP Tian, GL (reprint author), Metropolitan Water Reclamat Dist Greater Chicago, Monitoring & Res Dept, Environm Monitoring & Res Div, Lue Hing R&D Complex,6001 W Pershing Rd, Cicero, IL 60804 USA. EM guanglong.tian@mwrd.org NR 60 TC 3 Z9 3 U1 3 U2 32 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0929-1393 EI 1873-0272 J9 APPL SOIL ECOL JI Appl. Soil Ecol. PD JAN PY 2015 VL 85 BP 86 EP 93 DI 10.1016/j.apsoil.2014.09.001 PG 8 WC Soil Science SC Agriculture GA AU4OP UT WOS:000345591200011 ER PT J AU Manor, ML Weber, GM Cleveland, BM Yao, JB Kenney, PB AF Manor, Meghan L. Weber, Gregory M. Cleveland, Beth M. Yao, Jianbo Kenney, P. Brett TI Expression of genes associated with fatty acid metabolism during maturation in diploid and triploid female rainbow trout SO AQUACULTURE LA English DT Article DE Triploid; Maturation; Multiplex-GeXP; Fatty acid metabolism; mTOR; PPAR ID ONCORHYNCHUS-MYKISS; SEXUAL-MATURATION; PROXIMATE COMPOSITION; MAMMALIAN TARGET; LIPID-METABOLISM; DIFFERENT DIETS; ADIPOSE-TISSUE; POLYPLOID FISH; ENERGY STORES; MUSCLE AB To study the effects of sexual maturation on fatty acid metabolism in fish fed to satiation daily, expression of thirty-five genes involved in fatty acid metabolism was determined in sexually maturing diploid (2N; fertile) and triploid (3N; sterile) female rainbow trout. Gene expression was assessed in liver, white muscle, and visceral adipose tissues for fish that were 16 to 24 M of age. Previously, we reported minimal differences inmost growth measurements between maturing 2N and 3N fish, but there were disparate changes in muscle proximate composition, visceral fat stores, and fatty acid contents of energy stores at 21 M with 3N females having greater lipid stores. Here, we report that gene expression profiles of liver and white muscle corresponded to the phenotypes with significant differences in expression at 20 M. Triploid females had increased expression of genes involved in fatty acid synthesis; including gpat, srebp1, acyl, acc, fas, and scd1 in liver and fas in muscle. Conversely, 2N muscle had increased expression of beta-oxidation genes cpt1b, cpt2, ehhadh, and acat2 and TORC1 inhibitors redd1, erk, mo25, and pras40. Diploid muscle also had increased expression of ppar beta along with increased expression of the fatty acid transporter gene cd36, and beta-oxidation genes cpt1a, cpt1c, aco, and acdhvl at 20 M. Additionally, 2N visceral adipose tissue had increased cpt1a expression at 21 M. Overall, data suggest that 3N females are undergoing higher levels of fatty acid synthesis while 2N females have higher levels of beta-oxidation during sexual maturation. Phenotypic data supports these findings with decreasing fatty acid stores in 2N females during this time period. Additionally, changes in gene expression are associated with altered expression within the mTOR and PPAR beta signaling pathways. (C) 2014 Elsevier B.V. All rights reserved. C1 [Manor, Meghan L.; Yao, Jianbo; Kenney, P. Brett] W Virginia Univ, Davis Coll Agr Nat Resources & Design, Div Anim & Nutr Sci, Morgantown, WV 26506 USA. [Weber, Gregory M.; Cleveland, Beth M.] USDA ARS, Natl Ctr Cool & Cold Water Aquaculture, Kearneysville, WV 25430 USA. RP Manor, ML (reprint author), W Virginia Univ, Div Anim & Nutr Sci, 1042 Agr Sci Bldg POB 6108, Morgantown, WV 26505 USA. EM mmanor@mix.wvu.edu FU USDA/CSREES [2007-35205-17914]; USDA/ARS CRIS [1930-31000-010-000D]; USDA-ARS [58-1930-0-059] FX l This study was funded by USDA/CSREES#2007-35205-17914, USDA-ARS Cooperative Agreement No. 58-1930-0-059, and supported by USDA/ARS CRIS Project 1930-31000-010-000D. It is published with the approval of the West Virginia University Director of the Agricultural Station as scientific paper nr 3218. NR 35 TC 3 Z9 3 U1 1 U2 22 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 1 PY 2015 VL 435 BP 178 EP 186 DI 10.1016/j.aquaculture.2014.09.026 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AT9EA UT WOS:000345229500026 ER PT J AU Straus, DL Farmer, BD Beck, BH Bosworth, BG Torrans, EL Tucker, CS AF Straus, David L. Farmer, Bradley D. Beck, Benjamin H. Bosworth, Brian G. Torrans, Eugene L. Tucker, Craig S. TI Water hardness influences Flavobacterium columnare pathogenesis in channel catfish SO AQUACULTURE LA English DT Article DE Flavobacterium columnare; Channel catfish; Pathogenesis; Water chemistry ID ICTALURUS-PUNCTATUS RAFINESQUE; FLEXIBACTER-COLUMNARIS; EXPERIMENTAL-INFECTION; EDWARDSIELLA-ICTALURI; IMMERSION CHALLENGE; BIOFILM FORMATION; MAGNESIUM-IONS; FISH; CALCIUM; ADHESION AB Studies were conducted to determine aspects of water chemistry responsible for large differences in pathogenesis and mortality rates in experimental challenges of channel catfish Ictalurus punctatus with Flavobacterium columnare; challenges were conducted in water supplying either the Stuttgart National Aquaculture Research Center (SNARC) in Stuttgart, Arkansas, or the Warmwater Aquaculture Research Unit (WARU) in Stoneville, Mississippi. Waters differed in two major characteristics: compared to SNARC water, WARU water contained appreciable dissolved organic matter (DOM) and very low concentrations of dissolved calcium and magnesium. In Experiment 1, fish were exposed to a bacterial challenge of F. columnare in aquaria with either SNARC or WARU water. Fish mortality at 4 d was 100% for fish in SNARC water, while no fish died in WARU water. Bacterial adhesion to gill surfaces also differed dramatically in the two waters and was approximately 1900-times higher for fish challenged in SNARC water (more than 800,000 bacterial CFUs/ng extracted DNA) than for fish challenged in WARU water (fewer than 450 CFUs/ng). Experiment 2 was conducted with a lower bacterial challenge to determine which factor, DOM or divalent cation concentration, caused the difference in mortality seen in Experiment 1. Complete removal of DOM from WARU water by carbon filtration did not affect bacterial adhesion to gills nor resulted in greater mortality in challenged fish. Consequently, DOM did not contribute to the differences in mortality seen in Experiment 1. However, removal of most calcium and magnesium from SNARC water by ion-exchange filtration resulted in decreased bacterial adhesion to gill and decreased mortality compared to un-altered SNARC water. It appears that the concentration of divalent cations (hardness) affects the pathogenesis of columnaris disease. Published by Elsevier B.V. C1 [Straus, David L.; Farmer, Bradley D.; Beck, Benjamin H.] USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. [Bosworth, Brian G.; Torrans, Eugene L.; Tucker, Craig S.] USDA ARS, Warmwater Aquaculture Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA. RP Straus, DL (reprint author), USDA ARS, Harry K Dupree Stuttgart Natl Aquaculture Res Ctr, Stuttgart, AR 72160 USA. EM dave.straus@ars.usda.gov FU USDA/ARS under CRIS [6225-32000-004-00D] FX Special thanks to George Huskey at SNARC for providing channel catfish for this study. Lab Assistant Cindy Ledbetter maintained the fish and assisted in the experiments. Dr. Carl Webster provided a review of the manuscript. Work funded by USDA/ARS under CRIS # 6225-32000-004-00D. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U. S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 46 TC 1 Z9 1 U1 3 U2 17 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 1 PY 2015 VL 435 BP 252 EP 256 DI 10.1016/j.aquaculture.2014.10.003 PG 5 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AT9EA UT WOS:000345229500035 ER PT J AU Buentello, A Jirsa, D Barrows, FT Drawbridge, M AF Buentello, Alejandro Jirsa, Dave Barrows, Frederic T. Drawbridge, Mark TI Minimizing fishmeal use in juvenile California yellowtail, Seriola lalandi, diets using non-GM soybeans selectively bred for aquafeeds SO AQUACULTURE LA English DT Article DE Yellowtail; Seriola lalandi; Novel non-GM soybean meal; Fishmeal replacement ID TROUT ONCORHYNCHUS-MYKISS; SOY PROTEIN-CONCENTRATE; SALMON SALMO-SALAR; OPTIMUM FEEDING FREQUENCY; WINTER-BASED ASSESSMENT; CYPRINUS-CARPIO L.; BY-PRODUCT MEAL; ATLANTIC SALMON; GROWTH-PERFORMANCE; NUTRIENT DIGESTIBILITY AB A study was undertaken to examine the potential of two non-genetically modified (non-GM) soy products, differentially processed, as fishmeal (FM) replacements. Dietary protein (DP) was replaced at 50, 60 and 70% using the 3011 meal or 40, 50 and 60% with the 3032 meal. Ingredient 3011 was a cooked and solvent-extracted meal from a high protein and low oligosaccharide soy cultivar; whereas, ingredient 3032 was a cold-pressed, low fat cake ground to a meal that originated from a medium protein, low oligosaccharide, low protease inhibitor soy cultivar. Experimental diets were isonitrogenous (45% crude protein, CP), isolipidic (13% lipid), isocaloric (12.6 kJ energy kg(-1) of diet) and were balanced for lysine (3.2%) and methionine + cysteine (2%). Taurine was supplemented at 1.5% in all diets. A commercial control diet (52% CP and 13% lipid) was included for a total of eight test diets. Twenty four round tanks (330 L) were each stocked with 20, 4 g yellowtail (Seriola lalandi). Tanks were supplied with recirculated seawater and each diet was randomly assigned to three replicate tanks. After a 10-week feeding trial, growth, survival, feed conversion ratio, protein efficiency ratio, protein retention, condition factor and carcass proximate composition were contrasted among dietary treatments. Results indicate that juvenile yellowtail fed diets in which the 3032 meal provided 50 and 60% of DP grew significantly (P < 0.001) better than those fed all other diets, including the FM-based (menhaden FM) and commercial control diets. Fish fed with the 3011 meal providing 50% of DP and those fed with the 3032 meal providing 40% of DP had similar growth performance which was slightly greater than the fish fed the FM reference diet. Minor intestinal histology differences were found among fish fed the 8 diets. Taken together these data indicates that the improved non-GM soybean meals supported better performance of juvenile yellowtail than fish fed the FM reference diet. No enteritis was detected even at high dietary inclusion levels. (C) 2014 Elsevier B.V. All rights reserved. C1 [Buentello, Alejandro] Navita Premium Feed Ingredients Inc, W Des Moines, IA 50266 USA. [Jirsa, Dave; Drawbridge, Mark] Hubbs SeaWorld Res Inst, San Diego, CA 92109 USA. [Barrows, Frederic T.] USDA ARS, Hagerman Fish Culture Expt Stn, Hagerman, ID 83332 USA. RP Buentello, A (reprint author), Navita Premium Feed Ingredients Inc, 4401 Westown Pkwy,Suite 225, W Des Moines, IA 50266 USA. EM alex-buentello@hotmail.com FU Nebraska Soybean Association; Iowa Soybean Association FX The authors would like to express their gratitude to staff at HSWRI (Jose Velaquez, Andrea Marino and Nicole Grimaud) for helping with the experiment take down as well as staff from the USDA/ARS Bozeman, MT (Jason Frost and Carley Stone) for heat-deactivation and diet manufacturing. This research was funded by in part by the Nebraska Soybean Association and the Iowa Soybean Association. NR 83 TC 3 Z9 4 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 1 PY 2015 VL 435 BP 403 EP 411 DI 10.1016/j.aquaculture.2014.10.027 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AT9EA UT WOS:000345229500056 ER PT J AU Riche, M AF Riche, Marty TI Nitrogen utilization from diets with refined and blended poultry by-products as partial fish meal replacements in diets for low-salinity cultured Florida pompano, Trachinotus carolinus SO AQUACULTURE LA English DT Article DE Nitrogen retention; Poultry by-products; Fish meal replacement; Ammonia excretion; Methionine; Urea ID TROUT ONCORHYNCHUS-MYKISS; BASS DICENTRARCHUS-LABRAX; TURBOT PSETTA-MAXIMA; EUROPEAN SEA BASS; CLARIAS-GARIEPINUS BURCHELL; ANIMAL PROTEIN INGREDIENTS; SPARUS-AURATA L; RAINBOW-TROUT; PRACTICAL DIETS; DIGESTIBILITY COEFFICIENTS AB Three trials were performed to evaluate partial fish meal (FM) replacement with poultry by-products in a practical-type diet for Florida pompano, Trachinotus carolinus reared in low-salinity. Two blended poultry by-product meals (BP67, BP70), two blended chicken concentrates (CC66, CC70) and one standard pet-food grade poultry by-product meal (E150) were evaluated. The diets were formulated to contain 48% crude protein (CP) and 21 MJ/kg. Poultry by-products replaced 67% of the FM protein on an isonitrogenous basis. Trial 1 was a 10 week growth trial with juvenile (3.3 +/- 0.10 g) Florida pompano. No differences were detected in growth, efficiency, feed intake, protein productive value, or body composition between the by-product diets and a FM reference diet. Trial 2 examined protein, energy, dry matter, and organic matter digestibility and amino acid availability in 50.0 +/- 3.8 g Florida pompano. Apparent CP digestibility (65.6-72.9%) was significantly lower (P < 0.05) in the BP67 diet than the others, except BP70. No other differences were detected. In trial 3, Florida pompano (8.1 +/- 0.66 g) were fed a morning meal, and postprandial total ammonia-N (TAN) and urea excretion measured at 8 hourly increments. Ammonia increased 3.1-3.4 fold within 1 h. Rate of TAN excretion (mg TAN/kg.h) did not significantly decrease until 4-5 h postprandially. No differences in TAN accumulation were observed until 4 h when accumulation was significantly higher (P < 0.05) in CC66, and at 5 h lower in CC70 relative to the FM diet. Rate of urea excretion was constant. A slope ratio analysis indicated there were no differences in urea excretion among the treatments. Stepwise multiple regression suggested that methionine and lysine were the first and second limiting amino acids explaining 99.7% of the variability in weight gain. It is concluded that poultry by-products are suitable partial replacements for FM in Florida pompano diets. (C) 2014 Elsevier B.V. All rights reserved. C1 [Riche, Marty] USDA ARS, Ft Pierce, FL 34946 USA. RP Riche, M (reprint author), Florida Atlantic Univ, Oceanog Inst, Harbor Branch, 5600 US Hwy 1,North, Ft Pierce, FL 34946 USA. EM richem@fau.edu FU USDA/Agricultural Research Project [6225-63000-007-00D] FX I would like to acknowledge the contributions and technical support provided by David Haley and Patrick Tracy of USDA, ARS, and Terhea Williams and Jason Franklin of Florida Atlantic University's Harbor Branch Oceanographic Institute, Fort Pierce, FL. This study was funded by the USDA/Agricultural Research Project No. 6225-63000-007-00D. All programs and services of the USDA are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age, marital status or handicap. Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply endorsement by the USDA. NR 64 TC 1 Z9 2 U1 0 U2 13 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0044-8486 EI 1873-5622 J9 AQUACULTURE JI Aquaculture PD JAN 1 PY 2015 VL 435 BP 458 EP 466 DI 10.1016/j.aquaculture.2014.10.001 PG 9 WC Fisheries; Marine & Freshwater Biology SC Fisheries; Marine & Freshwater Biology GA AT9EA UT WOS:000345229500063 ER PT J AU Sun, XX Sun, S Ference, C Zhu, WJ Zhou, NH Zhang, YF Zhou, KQ AF Sun, Xiuxiu Sun, Shi Ference, Christopher Zhu, Wenjun Zhou, Ninghui Zhang, Yifan Zhou, Kequan TI A potent antimicrobial compound isolated from Clathria cervicornis SO BIOORGANIC & MEDICINAL CHEMISTRY LETTERS LA English DT Article DE Natural antimicrobial; Clathria cervicornis; Crambescidin 800; Antimicrobial activity; Minimal inhibitory concentration ID RESISTANT PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; MARINE SPONGES; AGELAS SPONGES; RISK-FACTORS; ALKALOIDS; FLUOROQUINOLONES; SUSCEPTIBILITY; INFECTIONS; STRAINS AB The sponge of Clathria cervicornis is commonly used in traditional medicine. This study aims to identify the active compound in C. cervicornis and to evaluate its antimicrobial activity. The purified active compound was determined to be crambescidin 800 and was found to be highly active against Acinetobacter baumannii (minimal inhibitory concentration, MIC = 2 mu g/ml), Klebsiella pneumoniae (MIC = 1 mu g/ml) and Pseudomonas aeruginosa (MIC = 1 mu g/ml). A potent antimicrobial compound, crambescidin 800, was isolated in Clathria cervicornis. It is extremely active against three common pathogenic bacteria. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Sun, Xiuxiu; Sun, Shi; Zhu, Wenjun; Zhou, Ninghui; Zhang, Yifan; Zhou, Kequan] Wayne State Univ, Dept Nutr & Food Sci, Detroit, MI 48202 USA. [Ference, Christopher] ARS, USDA, USHRL, Ft Pierce, FL 34945 USA. RP Zhou, KQ (reprint author), Wayne State Univ, Dept Nutr & Food Sci, Detroit, MI 48202 USA. EM kzhou@wayne.edu RI Sun, Shi/P-6018-2014 FU Wayne State University Graduate Research Fellow (UGRF) FX This work was supported by Wayne State University Graduate Research Fellow (UGRF). NR 21 TC 1 Z9 1 U1 0 U2 11 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0960-894X EI 1464-3405 J9 BIOORG MED CHEM LETT JI Bioorg. Med. Chem. Lett. PD JAN 1 PY 2015 VL 25 IS 1 BP 67 EP 69 DI 10.1016/j.bmcl.2014.11.012 PG 3 WC Chemistry, Medicinal; Chemistry, Organic SC Pharmacology & Pharmacy; Chemistry GA AU4LD UT WOS:000345581600013 PM 25435146 ER PT J AU Li, ZW Zhang, GH Geng, R Wang, H Zhang, XC AF Li, Zhen-Wei Zhang, Guang-Hui Geng, Ren Wang, Hao Zhang, X. C. TI Land use impacts on soil detachment capacity by overland flow in the Loess Plateau, China SO CATENA LA English DT Article DE Soil erosion; Detachment capacity; Soil type; Land use; The Loess Plateau ID CONCENTRATED FLOW; SHALLOW FLOW; STEEP SLOPES; EROSION; ERODIBILITY; RESISTANCE; ROOTS; WATER; PREDICTION; CATCHMENT AB Land use and its adjustment may greatly affect soil detachment process by overland flow via altering soil properties, root systems, and tillage operations, but few studies were performed to quantify their effects on soil detachment in the Loess Plateau. This study was conducted to investigate the potential effects of land use on soil detachment capacity by overland flow (D-c, kg m(-2) S-1) using natural undisturbed soil samples taken from four different land uses on the red loess soil and six different land uses on the yellow loess soil, and to quantify the relationships between soil detachment capacity and hydraulic parameters, soil properties, and root systems in the Loess Plateau. The collected samples were tested in a 4.0 m long, 035 m wide hydraulic flume under six different shear stresses (5.51-16.59 Pa). The result showed that both soil type and land use had significant effects on D-c. For two tested soils, the mean D-c of the yellow loess soil was 1.49 times greater than that of the red loess soil. For the red loess soil, D-c of cropland was the maximum, which was 5.57, 5.85, and 34.08 times greater than those of shrub land, orchard, and grassland, respectively. For the yellow loess soil, cropland was much more erodible than other five land uses. On average, the ratios of the cropland D-c to those of orchard, shrub land, woodland, grassland, and wasteland were 7.14, 12.29, 25.78, 28.45, and 46.43, respectively. The variability of D-c under different land uses was closely related to soil properties, root systems, and tillage operations. Soil detachment capacity was positively related to silt content, and inversely related to sand content, cohesion, water stable aggregate, aggregate median diameter, organic matter, and root density. The measured detachment capacity could be well estimated by measurable parameters of stream power, slope gradient, soil bulk density, median diameter, silt content, cohesion, and root density (Nash-Sutcliffe efficiency = 0.89). (C) 2014 Elsevier B.V. All rights reserved. C1 [Li, Zhen-Wei; Zhang, Guang-Hui] Chinese Acad Sci, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. [Li, Zhen-Wei; Zhang, Guang-Hui] Minist Water Resources, Yangling 712100, Shaanxi, Peoples R China. [Li, Zhen-Wei] Univ Chinese Acad Sci, Beijing 100049, Peoples R China. [Zhang, Guang-Hui; Geng, Ren; Wang, Hao] Being Normal Univ, Sch Geog, Beijing 100875, Peoples R China. [Zhang, X. C.] USDA ARS, Grazinglands Res Lab, El Reno, OK USA. RP Zhang, GH (reprint author), Chinese Acad Sci, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China. EM ghzhang@bnu.edu.cn FU Hundred Talents Project of the Chinese Academy of Sciences; National Natural Science Foundation of China [41271287, 41301295] FX Financial assistance for this study was provided by the Hundred Talents Project of the Chinese Academy of Sciences and the National Natural Science Foundation of China (41271287 and 41301295). The authors thank the members of the Ansai Station of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Recourses for their assistance. NR 40 TC 10 Z9 10 U1 2 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0341-8162 EI 1872-6887 J9 CATENA JI Catena PD JAN PY 2015 VL 124 BP 9 EP 17 DI 10.1016/j.catena.2014.08.019 PG 9 WC Geosciences, Multidisciplinary; Soil Science; Water Resources SC Geology; Agriculture; Water Resources GA AU3WJ UT WOS:000345541200002 ER PT J AU Moscetti, R Haff, RP Stella, E Contini, M Monarca, D Cecchini, M Massantini, R AF Moscetti, Roberto Haff, Ron P. Stella, Elisabetta Contini, Marina Monarca, Danilo Cecchini, Massimo Massantini, Riccardo TI Feasibility of NIR spectroscopy to detect olive fruit infested by Bactrocera oleae SO POSTHARVEST BIOLOGY AND TECHNOLOGY LA English DT Article DE Olea europaea L.; Insect damage; Acousto-optic tunable filter-near infrared spectroscopy; Discriminant analysis; Wavelengths selection ID NEAR-INFRARED SPECTROSCOPY; INTERNAL INSECT INFESTATION; REFLECTANCE SPECTROSCOPY; FLY INFESTATION; QUALITY; OIL; KERNELS; CLASSIFICATION; TRANSMITTANCE; CHESTNUTS AB Olive fruit fly infestation is a significant problem for the milling process. In most cases, damage from insects is 'hidden', i.e. not visually detectable on the fruit surface. Consequently, traditional visual sorting techniques are generally inadequate for the detection and removal of olives with insect damage. In this study, the feasibility of using NIR spectroscopy to detect hidden insect damage is demonstrated. Using a genetic algorithm for feature selection (from 2 to 6 wavelengths) in combination with linear discriminant analysis (LDA), quadratic discriminant analysis (QDA) or k-nearest-neighbors (kNN) routines, classification error rates as low as 0.00% false negative, 12.50% false positive, and 6.25% total error were achieved, with an AUC value of 0.9766 and a Wilk's lambda of 0.3686 (P < 0.001). Multiplicative scatter correction, Savitzky-Golay spectral pre-treatment with 13 smoothing points and mean centering spectral pre-treatments were used. The optimal features corresponded to Abs[1108 nm], Abs[1232 nm], Abs[1416 nm], Abs[1486 nm] and Abs[2148 nm]. (C) 2014 Elsevier B.V. All rights reserved. C1 [Moscetti, Roberto; Stella, Elisabetta; Monarca, Danilo; Cecchini, Massimo] Univ Tuscia, Dept Sci & Technol Agr Forest Nat & Energy, I-01100 Viterbo, Italy. [Haff, Ron P.] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Contini, Marina; Massantini, Riccardo] Univ Tuscia, Dept Innovat Biol Agrofood & Forest Syst, I-01100 Viterbo, Italy. RP Massantini, R (reprint author), Univ Tuscia, Dept Innovat Biol Agrofood & Forest Syst, Via S Camillo de Lellis Snc, I-01100 Viterbo, Italy. EM massanti@unitus.it RI Cecchini, Massimo/F-3411-2012; Monarca, Danilo/B-8010-2016; OI Cecchini, Massimo/0000-0003-1407-8127; Massantini, Riccardo/0000-0001-6517-3162; Monarca, Danilo/0000-0002-8980-3744; Moscetti, Roberto/0000-0002-2034-8536 NR 41 TC 9 Z9 9 U1 5 U2 28 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0925-5214 EI 1873-2356 J9 POSTHARVEST BIOL TEC JI Postharvest Biol. Technol. PD JAN PY 2015 VL 99 BP 58 EP 62 DI 10.1016/j.postharvbio.2014.07.015 PG 5 WC Agronomy; Food Science & Technology; Horticulture SC Agriculture; Food Science & Technology GA AU3DP UT WOS:000345493200008 ER PT J AU Hooie, NA Wiggins, GJ Lambdin, PL Grant, JF Powell, SD Lelito, JP AF Hooie, Nicholas A. Wiggins, Gregory J. Lambdin, Paris L. Grant, Jerome F. Powell, Steve D. Lelito, Jonathan P. TI Native parasitoids and recovery of Spathius agrili from areas of release against emerald ash borer in eastern Tennessee, USA SO BIOCONTROL SCIENCE AND TECHNOLOGY LA English DT Article DE emerald ash borer; Agrilus planipennis; Spathius agrili; Spathius floridanus; Atanycolus cappaerti; parasitoid ID NEES HYMENOPTERA-BRACONIDAE; COLEOPTERA BUPRESTIDAE; SASAJISCYMNUS-TSUGAE; ESTABLISHMENT; PLANIPENNIS; DORYCTINAE; ABUNDANCE; HEMLOCK AB The parasitoid Spathius agrili Yang, introduced in the USA to suppress populations of the emerald ash borer (EAB), Agrilus planipennis Fairmaire, has been recovered at a release site for the first time in eastern Tennessee after a single year of releases. Other native parasitoids, including Spathius floridanus Ashmead, undetermined species of Spathius (possibly Spathius elegans Matthews and Spathius parvulus Matthews) and Atanycolus cappaerti Marsh & Strazanac, also known to be associated with EAB, were recovered. These recoveries represent the first documentation of these four species, including the introduced S. agrili, associated with EAB in the southern USA. Implications for biological control efforts against EAB are discussed. C1 [Hooie, Nicholas A.; Wiggins, Gregory J.; Lambdin, Paris L.; Grant, Jerome F.] Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37901 USA. [Powell, Steve D.] Ellington Agr Ctr, Tennessee Dept Agr, Nashville, TN USA. [Lelito, Jonathan P.] AHPIS, Emerald Ash Borer Program, Biol Control Facil, USDA,PPQ, Brighton, MI USA. RP Hooie, NA (reprint author), Univ Tennessee, Dept Entomol & Plant Pathol, Knoxville, TN 37901 USA. FU USDA APHIS [11-8130-0079-CA] FX This research was partially funded by USDA APHIS [grant number 11-8130-0079-CA]. NR 20 TC 4 Z9 5 U1 3 U2 34 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0958-3157 EI 1360-0478 J9 BIOCONTROL SCI TECHN JI Biocontrol Sci. Technol. PY 2015 VL 25 IS 3 BP 345 EP 351 DI 10.1080/09583157.2014.971712 PG 7 WC Biotechnology & Applied Microbiology; Entomology SC Biotechnology & Applied Microbiology; Entomology GA AT5UQ UT WOS:000345008200009 ER PT J AU Simmons, RW Chaney, RL Angle, JS Kruatrachue, M Klinphoklap, S Reeves, RD Bellamy, P AF Simmons, R. W. Chaney, R. L. Angle, J. S. Kruatrachue, M. Klinphoklap, S. Reeves, R. D. Bellamy, P. TI Towards Practical Cadmium Phytoextraction with Noccaea Caerulescens SO INTERNATIONAL JOURNAL OF PHYTOREMEDIATION LA English DT Article DE cadmium; zinc; phytoextraction; Noccaea caerulescens; Thailand ID HYPERACCUMULATOR THLASPI-CAERULESCENS; METAL-CONTAMINATED SOILS; RICE GRAIN; ROOT DEVELOPMENT; DIETARY-CADMIUM; POLLUTED SOILS; FIELD TRIALS; ZINC; CD; ZN AB A series of field trials were conducted to investigate the potential of Noccaea caerulescens F.K. Mey [syn. Thlaspi caerulescens J & C Presl. (seeKoch and Al-Shehbaz 2004)] populations (genotypes) derived from southern France to phytoextract localized Cd/Zn contamination in Thailand. Soil treatments included pH variation and fertilization level and application of fungicide. N. caerulescens populations were transplanted to the field plots three months after germination and harvested in May, prior to the onset of seasonal rains. During this period growth was rapid with shoot biomass ranging from 0.93-2.2 g plant(-1) (280-650 kg ha(-1)) DW. Shoot Cd and Zn concentrations for the four populations evaluated ranged from 460-600 and 2600-2900 mg kg(-1) DW respectively. Cadmium and Zn Translocation Factors (shoot/root) for the populations tested ranged from 0.91-1.0 and 1.7-2.1 and Bioaccumulation Factors ranged from 12-15 and 1.2-1.3. We conclude that optimizing the use of fungicidal sprays, acidic soil pH, planting density and increasing the effective cropping period will increase rates of Cd and Zn removal enough to facilitate practical Cd phytoextraction from rice paddy soils in Thailand. C1 [Simmons, R. W.; Bellamy, P.] Cranfield Univ, Dept Agri Food, Cranfield MK43 0AL, Beds, England. [Chaney, R. L.] USDA ARS, Environm Management & Byprod Utilizat Lab, Beltsville, MD USA. [Angle, J. S.] Univ Georgia, Coll Agr & Environm Sci, Athens, GA 30602 USA. [Klinphoklap, S.] Dept Agr, Bangkok, Thailand. [Reeves, R. D.] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia. RP Simmons, RW (reprint author), Cranfield Univ, Cranfield MK43 0AL, Beds, England. EM r.w.simmons@cranfield.ac.uk OI Simmons, Robert/0000-0002-9594-9934; Bellamy, Patricia/0000-0003-4116-3837 FU United States Department of Agriculture [RB303-004/3505988]; International Water Management Institute FX The authors would like to acknowledge the financial support provided by the United States Department of Agriculture (Sub-award No. RB303-004/3505988) and the International Water Management Institute without which this research would not have been possible. NR 58 TC 4 Z9 4 U1 5 U2 27 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1522-6514 EI 1549-7879 J9 INT J PHYTOREMEDIAT JI Int. J. Phytoremediat. PY 2015 VL 17 IS 2 BP 191 EP 199 DI 10.1080/15226514.2013.876961 PG 9 WC Environmental Sciences SC Environmental Sciences & Ecology GA AT3AQ UT WOS:000344807300012 PM 25360891 ER PT J AU Martinez-Sanz, M Bilbao-Sainz, C Du, WX Chiou, BS Williams, TG Wood, DF Imam, SH Orts, WJ Lopez-Rubio, A Lagaron, JM AF Martinez-Sanz, Marta Bilbao-Sainz, Cristina Du, Wen-Xian Chiou, Bor-Sen Williams, Tina G. Wood, Delilah F. Imam, Syed H. Orts, William J. Lopez-Rubio, Amparo Lagaron, Jose M. TI Antimicrobial Poly(lactic acid)-Based Nanofibres Developed by Solution Blow Spinning SO JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY LA English DT Article DE Bacterial Cellulose; Nanowhiskers; Blow Spinning; Poly(lactic acid); Antimicrobial Properties ID 4 MODEL-DRUGS; CELLULOSE NANOWHISKERS; NANOCOMPOSITE FIBERS; RELEASE CHARACTERISTICS; MECHANICAL-PROPERTIES; ELECTROSPUN FIBERS; ACID); NANOPARTICLES; NANOCRYSTALS; POLYLACTIDE AB The present study reports on the development of hybrid poly(lactic acid) (PLA) fibres loaded with highly crystalline bacterial cellulose nanowhiskers (BCNW) by the novel solution blow spinning method. Furthermore, fibres with antimicrobial properties were generated by incorporating carvacrol and THC as antimicrobial agents and the biocide effect against Listeria monocytogenes was studied. Initially, PLA blow spun fibres containing BCNW were optimized in terms of morphology and thermal properties. The addition of BCNW was seen to significantly increase the viscosity and surface tension of solutions, restricting the capacity to form fibres for concentrations greater than 30 wt.-% BCNW. 15 wt.-% BCNW was selected as the optimum nanofiller loading as it led to the most uniform fibres morphology, with BCNW homogeneously distributed along the fibres' axis. Subsequently, carvacrol and THC were incorporated into the fibres to confer them with antimicrobial properties, although the hydrophobic PLA matrix did not provide an efficient release of the antimicrobials. Thus, hydrophilic substances were added in order to trigger the antimicrobials release through water sorption mechanisms. The addition of the BCNW filler was not seen to significantly increase the antimicrobial capacity of the fibres by itself and, hence, gelatin was added to help promoting further the hydrophylicity and biocide performance of the fibres. Nevertheless, for the more hydrophilic THC, the biocide capacity of the fibres with gelatin was accentuated further by the presence of the BCNW. C1 [Martinez-Sanz, Marta; Lopez-Rubio, Amparo; Lagaron, Jose M.] CSIC, IATA, Novel Mat & Nanotechnol Grp, Valencia 46980, Spain. [Bilbao-Sainz, Cristina; Du, Wen-Xian; Chiou, Bor-Sen; Williams, Tina G.; Wood, Delilah F.; Imam, Syed H.; Orts, William J.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. RP Lagaron, JM (reprint author), CSIC, IATA, Novel Mat & Nanotechnol Grp, Avda Agustin Escardino 7, Valencia 46980, Spain. RI Lopez-Rubio, Amparo/H-2841-2012 OI Lopez-Rubio, Amparo/0000-0001-6469-9402 FU Spanish Ministry of Education; Spanish Ministry of Science and Innovation; EU FP7 ECOBIOCAP project; MICINN project [MAT2009-14533-C02-01] FX Marta Martinez-Sanz would like to thank the Spanish Ministry of Education for the FPU grant. Amparo Lopez-Rubio is the recipient of a "Ramon Y. Cajal" contract from the Spanish Ministry of Science and Innovation. The authors acknowledge financial support from the EU FP7 ECOBIOCAP project and from MICINN MAT2009-14533-C02-01 project. Collaboration with researchers at the Bioproduct Chemistry and Engineering Research Laboratory of the WRRC-USDA in Albany, CA is gratefully acknowledged. NR 50 TC 4 Z9 4 U1 0 U2 40 PU AMER SCIENTIFIC PUBLISHERS PI VALENCIA PA 26650 THE OLD RD, STE 208, VALENCIA, CA 91381-0751 USA SN 1533-4880 EI 1533-4899 J9 J NANOSCI NANOTECHNO JI J. Nanosci. Nanotechnol. PD JAN PY 2015 VL 15 IS 1 BP 616 EP 627 DI 10.1166/jnn.2015.9160 PG 12 WC Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied; Physics, Condensed Matter SC Chemistry; Science & Technology - Other Topics; Materials Science; Physics GA AT6NA UT WOS:000345054000114 PM 26328414 ER PT J AU Wang, YL Li, F Zhuang, H Chen, X Li, LH Qiao, WW Zhang, JH AF Wang, Yongli Li, Feng Zhuang, Hong Chen, Xiao Li, Lianghao Qiao, Weiwei Zhang, Jianhao TI Effects of plant polyphenols and alpha-tocopherol on lipid oxidation, residual nitrites, biogenic amines, and N-nitrosamines formation during ripening and storage of dry-cured bacon SO LWT-FOOD SCIENCE AND TECHNOLOGY LA English DT Article DE Dry-cured bacon; Polyphenols; Nitrite; Biogenic amines; N-Nitrosamines ID SHELF-LIFE; FERMENTED SAUSAGES; GREEN TEA; QUALITY CHARACTERISTICS; NATURAL ANTIOXIDANTS; PORK SAUSAGES; EXTRACTS; BEEF; ACID; ADDITIVES AB Effects of plant polyphenols (green tea polyphenols (GTP) and grape seed extract (GSE)) and alpha-tocopherol on physicochemical parameters, lipid oxidation, residual nitrite, microbiological counts, biogenic amines, and N-nitrosamines were determined in bacons during dry-curing and storage. Results show that plant polyphenols and alpha-tocopherol significantly decreased pH, lipid oxidation (formation of thiobarbituric acid reactive substances) and residual nitrite content compared with control (P < 0.05) at the end of ripening. Microbial counts, biogenic amines, and N-nitrosodimethylamine (NDMA) contents in dry-cured bacons were significantly affected by plant polyphenols or alpha-tocopherol (P < 0.05), with GTP being the most effective in reducing aerobic plate counts (APC), Enterobacteriaceae, and biogenic amines (putrescine, cadaverine, histamine, tyramine and spermine), as well as in inhibiting the NDMA formation (P < 0.05). Principal component analysis indicated that there were positive correlations between physicochemical factors, biogenic amines (putrescine, cadaverine, histamine, tyramine and spermine), NDMA and APC, and between the TBARS and sodium nitrite. There was a negative correlation between NDMA and nitrite. These findings suggest plant polyphenols, especially GTP, could be utilized for processing dry-cured bacons to improve quality, shelf life, and safety of finished products. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Wang, Yongli; Chen, Xiao; Li, Lianghao; Qiao, Weiwei; Zhang, Jianhao] Natl Engineer Res Ctr Meat Qual & Safety Control, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Yongli; Chen, Xiao; Li, Lianghao; Zhang, Jianhao] Minist Agr, Key Lab Meat Proc & Qual Control, Nanjing 210095, Jiangsu, Peoples R China. [Wang, Yongli; Chen, Xiao; Li, Lianghao; Zhang, Jianhao] Nanjing Agr Univ, Synerget Innovat Ctr Food Safety & Nutr, Nanjing 210095, Jiangsu, Peoples R China. [Li, Feng] Shandong Agr Univ, Coll Food Sci & Engn, Tai An 271018, Shandong, Peoples R China. [Zhuang, Hong] ARS, USDA, Qual & Safety Assessment Res Unit, Athens, GA 30605 USA. RP Zhang, JH (reprint author), Nanjing Agr Univ, Synerget Innovat Ctr Food Safety & Nutr, Nanjing 210095, Jiangsu, Peoples R China. EM jh_zhangnjau@163.com FU National Key Technology R&D Program in the 12th Five Year Plan of China [2012BAD28B01] FX This study was supported by the National Key Technology R&D Program in the 12th Five Year Plan of China (Grant 2012BAD28B01). NR 33 TC 9 Z9 11 U1 8 U2 58 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0023-6438 EI 1096-1127 J9 LWT-FOOD SCI TECHNOL JI LWT-Food Sci. Technol. PD JAN PY 2015 VL 60 IS 1 BP 199 EP 206 DI 10.1016/j.lwt.2014.09.022 PG 8 WC Food Science & Technology SC Food Science & Technology GA AT7HA UT WOS:000345106100029 ER PT J AU Pearson, MJ Monteith, SE Ferguson, RR Hallmark, CT Hudnall, WH Monger, HC Reinsch, TG West, LT AF Pearson, M. J. Monteith, S. E. Ferguson, R. R. Hallmark, C. T. Hudnall, W. H. Monger, H. C. Reinsch, T. G. West, L. T. TI A method to determine particle size distribution in soils with gypsum SO GEODERMA LA English DT Article DE Gypsiferous soils; Gypseous soils; Soil texture; Aridisols; Soil analysis ID STABILITY AB Gypseous (>40% gypsum) and gypsiferous (1 to 40% gypsum) soils are estimated to comprise from 100 million to 200 million ha globally including over 1 million ha in the U.S. More intensive land use on these soils has resulted in greater demand for information on distribution, properties, and behavior of these soils. Common laboratory methods for determining particle size distribution of soils involve pretreatment to remove gypsum and more soluble salts since they interfere with sample dispersion and establishment of a stable clay suspension. Thus, particle size measurements using standard methods with pretreatment to remove gypsum only reflect the size distribution of essentially insoluble, dominantly silicate minerals, and do not reflect the size distribution of the whole soil including gypsum. Forcing results into a silicate-mineral basis in moderate to high gypsum soils can profoundly distort analytical results. The objective of this project was to develop and evaluate an alternative method of particle size measurement for soils with gypsum that includes size distribution of gypsum particles. Three disaggregation methods for estimation of total sand and sand separates were evaluated: 1) drying sieving, 2) shaking in a 7:3 ethanol:water solution, and 3) sonication in a 7:3 ethanol:water solution. Evaluation of samples with and without gypsum indicated that the sonication method was the most effective for disaggregation as indicated by sand fraction distribution and microscopic grain evaluations. Application of the method to samples from three gypseous pedons yielded grain-size distributions that better agreed with hand textural class estimates than conventional grain-size analysis by standard methods that included pretreatment to remove gypsum. Comparison of CEC/clay and 1500 kPa water/clay ratios for the samples between the two methods also suggested that the proposed method yielded clay contents more consistent with other property data for the same horizons. Improved particle size evaluations for soils containing gypsum, especially those with high gypsum contents, will improve the understanding and ability to interpret and predict behavior of these unique soils. (C) 2014 Elsevier B.V. All rights reserved. C1 [Pearson, M. J.; Monteith, S. E.; Ferguson, R. R.; West, L. T.] USDA, NRCS, Natl Soil Survey Ctr, Lincoln, NE 68502 USA. [Hallmark, C. T.] Texas A&M Univ, College Stn, TX 77843 USA. [Hudnall, W. H.] Texas Tech Univ, Plant & Soil Sci Dept, Lubbock, TX 79409 USA. [Monger, H. C.] New Mexico State Univ, Dept Plant & Environm Sci, Las Cruces, NM 88003 USA. [Reinsch, T. G.] USDA, NRCS, World Soil Resources, Beltsville, MD 20705 USA. RP West, LT (reprint author), USDA, NRCS, Natl Soil Survey Ctr, 100 Centennial Mall N, Lincoln, NE 68502 USA. EM larrywestar@gmail.com NR 23 TC 0 Z9 0 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0016-7061 EI 1872-6259 J9 GEODERMA JI Geoderma PD JAN PY 2015 VL 237 BP 318 EP 324 DI 10.1016/j.geoderma.2014.09.016 PG 7 WC Soil Science SC Agriculture GA AS3YT UT WOS:000344211700031 ER PT J AU Vidogbena, F Adegbidi, A Tossou, R Assogba-Komlan, F Ngouajio, M Martin, T Simon, S Parrot, L Zander, KK AF Vidogbena, Faustin Adegbidi, Anselme Tossou, Rigobert Assogba-Komlan, Francoise Ngouajio, Mathieu Martin, Thibaut Simon, Serge Parrot, Laurent Zander, Kerstin K. TI Control of vegetable pests in Benin - Farmers' preferences for eco-friendly nets as an alternative to insecticides SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Benefit-cost analysis; Choice experiment; Integrated pest management; Pesticides; Insect net; Stated preference; Urban agriculture; West Africa ID PESTICIDE USE; CHOICE EXPERIMENT; FOOD SECURITY; WEST-AFRICA; MIXED LOGIT; SCALE HETEROGENEITY; CABBAGE PRODUCTION; URBAN AGRICULTURE; WILLINGNESS; MANAGEMENT AB We investigated if eco-friendly nets (EFNs) are a viable and acceptable alternative to extremely high levels of insecticide use in vegetable production. Using a choice experiment, we found that vegetable producing farmers in Benin preferred all of the characteristics of EFNs except the higher labor requirements. The nets had been distributed in a trial phase for free but in the long run farmers would need to purchase the EFNs. The break-even point for investing in nets was found to vary with the lifespan of EFNs, their purchase price and potential health benefits from avoiding large quantities of insecticides. To break even the nets need to be used for at least two production cycles. To overcome risk-averse farmer's reluctance to adopt EFNs we propose a credit and warranty scheme along with the purchase of the nets. The study's findings can guide the implementation of EFNs in other African countries as part of integrated pest management with global benefits for the environment and human health. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Vidogbena, Faustin] Reg Agr Ctr Rural Dev Atlantique Littoral, Abomey Calavi, Benin. [Adegbidi, Anselme; Tossou, Rigobert] Univ Abomey Calavi, Fac Sci Agron, Cotonou, Benin. [Assogba-Komlan, Francoise; Simon, Serge] Natl Agr Res Inst INRAB, Stn Agonkanmey, Cotonou, Benin. [Ngouajio, Mathieu] Michigan State Univ, Dept Hort, E Lansing, MI 48824 USA. [Ngouajio, Mathieu] USDA, NIFA, Washington, DC 20250 USA. [Martin, Thibaut; Simon, Serge; Parrot, Laurent] UR Hortsys, Cirad, Montpellier, France. [Martin, Thibaut] ICIPE, Plant Hlth Dept, Nairobi, Kenya. [Zander, Kerstin K.] Charles Darwin Univ, Northern Inst, Darwin, NT 0909, Australia. RP Zander, KK (reprint author), Charles Darwin Univ, Northern Inst, Ellengowan Dr, Darwin, NT 0909, Australia. EM kerstin.zander@cdu.edu.au OI Zander, Kerstin/0000-0002-2237-1801 FU American people through the U.S. Agency for International Development (USAID) [EPP-A-00-09-00004]; Michigan State University; Cirad-Agricultural Research for Development; Kenya Agricultural Research Institute (KARI); International Centre of Insect Physiology and Ecology (Icipe) in Kenya; A to Z Textile Mills in Tanzania; University of Abomey Calavi; Institut National des Recherches Agricoles du Benin (INRAB); Association des Personnes Renovatrices des Technologies Traditionnelles (APPRETECTRA) in Benin FX This study was made possible by the generous support of the American people through the U.S. Agency for International Development (USAID) under Award No. EPP-A-00-09-00004, with additional support by Michigan State University and the Cirad-Agricultural Research for Development. The contents are the responsibility of Horticulture Collaborative Research Support Program (HortCRSP) project BioNetAgro investigators and do not necessarily reflect the views of USAID or the U.S. Government. We also acknowledge our project partners: the Kenya Agricultural Research Institute (KARI) and the International Centre of Insect Physiology and Ecology (Icipe) in Kenya; A to Z Textile Mills in Tanzania; University of Abomey Calavi, Institut National des Recherches Agricoles du Benin (INRAB), and Association des Personnes Renovatrices des Technologies Traditionnelles (APPRETECTRA) in Benin for their support. We also wish to thank Stephen Garnett of Charles Darwin University for his editorial advice. NR 83 TC 3 Z9 3 U1 1 U2 24 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD JAN 1 PY 2015 VL 147 BP 95 EP 107 DI 10.1016/j.jenvman.2014.09.010 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA AS7FW UT WOS:000344423500010 PM 25262392 ER PT J AU Dorado, C Mullen, CA Boateng, AA AF Dorado, Christina Mullen, Charles A. Boateng, Akwasi A. TI Origin of carbon in aromatic and olefin products derived from HZSM-5 catalyzed co-pyrolysis of cellulose and plastics via isotopic labeling SO APPLIED CATALYSIS B-ENVIRONMENTAL LA English DT Article DE Catalytic fast pyrolysis; Isotopic labeling; Reaction mechanisms; Olefin; Aromatic ID LOW-DENSITY POLYETHYLENE; FLUIDIZED-BED REACTOR; LIGNOCELLULOSIC BIOMASS; REACTION PATHWAYS; CONVERSION; CRACKING; WASTE; ZSM-5; POLYSTYRENE; MECHANISM AB Catalytic pyrolysis over HZSM-5 is an effective method for the conversion of biomass to aromatic hydrocarbons, albeit with low yield and short catalyst lifetimes. Addition of co-reactants rich in carbon and hydrogen can enhance yield and possibly increase catalyst lifetimes by reducing coke formation. Particularly, the catalytic co-pyrolysis of plastic and biomass has been shown to enhance conversion to aromatic hydrocarbons, and also offers a method for productive disposal of waste agricultural plastics. In an effort to determine the origin of the carbon (plastic or biomass) in the products from this catalytic co-pyrolysis, mixtures of uniformly labeled C-13 cellulose and non-labeled plastic including polyethylene terephthalate, polypropylene, high density polyethylene, low density polyethylene and polystyrene were subjected to catalytic fast pyrolysis (CFP) at 650 degrees C in the presence of HZSM-5. A micro pyrolyzer coupled with GC/MS (py-GC/MS) advised product distributions and mass spectral data was used to determine the distribution of biogenic carbon and plastic derived carbon in the products. The results demonstrate that aromatic hydrocarbon products formed from the CFP of mixtures of cellulose and plastic are composed mostly of molecules containing carbon of mixed origin. Data on the distribution of C-13(x) C-12(y) from the products followed in this study show that polyolefin mixtures with cellulose favor the formation of alkyl benzenes that incorporate carbon from both sources. Utilization of aromatic polymers (polystyrene or polyethylene terephthalate) is more selective for formation of naphthalenes with carbon derived from both products. The distribution of various(13)C(x) C-12(y) products is used to suggest active mechanisms that result in the formation of the observed products. Published by Elsevier B.V. C1 [Dorado, Christina; Mullen, Charles A.; Boateng, Akwasi A.] USDA ARS, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Mullen, CA (reprint author), USDA ARS, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM charles.mullen@ars.usda.gov NR 49 TC 17 Z9 17 U1 7 U2 62 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0926-3373 EI 1873-3883 J9 APPL CATAL B-ENVIRON JI Appl. Catal. B-Environ. PD JAN PY 2015 VL 162 BP 338 EP 345 DI 10.1016/j.apcatb.2014.07.006 PG 8 WC Chemistry, Physical; Engineering, Environmental; Engineering, Chemical SC Chemistry; Engineering GA AR6IH UT WOS:000343686900040 ER PT S AU Bragg, JN Anderton, A Nieu, R Vogel, JP AF Bragg, Jennifer N. Anderton, Amy Nieu, Rita Vogel, John P. BE Wang, K TI Brachypodium distachyon SO AGROBACTERIUM PROTOCOLS, VOL 1, 3RD EDITION SE Methods in Molecular Biology LA English DT Article; Book Chapter DE Agrobacterium; Biofuel; Brachypodium; Embryogenic callus; Grass; Model system; T-DNA; Tissue culture; Transformation ID AGROBACTERIUM-MEDIATED TRANSFORMATION; DNA INSERTIONAL MUTAGENESIS; FUNCTIONAL GENOMICS; PLANTS; MODEL; RICE; ARABIDOPSIS; SYSTEM AB The small grass Brachypodium distachyon has attributes that make it an excellent model for the development and improvement of cereal crops and bioenergy feedstocks. To realize the potential of this system, many tools have been developed (e. g., the complete genome sequence, a large collection of natural accessions, a high density genetic map, BAC libraries, EST sequences, microarrays, etc.). In this chapter, we describe a high-efficiency transformation system, an essential tool for a modern model system. Our method utilizes the natural ability of Agrobacterium tumefaciens to transfer a well-defined region of DNA from its tumor-inducing (Ti) plasmid DNA into the genome of a host plant cell. Immature embryos dissected out of developing B. distachyon seeds generate an embryogenic callus that serves as the source material for transformation and regeneration of transgenic plants. Embryogenic callus is cocultivated with A. tumefaciens carrying a recombinant plasmid containing the desired transformation sequence. Following cocultivation, callus is transferred to selective media to identify and amplify the transgenic tissue. After 2-5 weeks on selection media, transgenic callus is moved onto regeneration media for 2-4 weeks until plantlets emerge. Plantlets are grown in tissue culture until they develop roots and are transplanted into soil. Transgenic plants can be transferred to soil 6-10 weeks after cocultivation. Using this method with hygromycin selection, transformation efficiencies average 42 %, and it is routinely observed that 50-75 % of cocultivated calluses produce transgenic plants. The time from dissecting out embryos to having the first transgenic plants in soil is 14-18 weeks, and the time to harvesting transgenic seeds is 20-31 weeks. C1 [Bragg, Jennifer N.; Anderton, Amy; Nieu, Rita] ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. [Vogel, John P.] US DOE, Joint Genome Inst, Walnut Creek, CA USA. RP Bragg, JN (reprint author), ARS, USDA, Western Reg Res Ctr, Albany, CA 94710 USA. OI Vogel, John/0000-0003-1786-2689 NR 21 TC 2 Z9 2 U1 1 U2 16 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA SN 1064-3745 BN 978-1-4939-1695-5; 978-1-4939-1694-8 J9 METHODS MOL BIOL JI Methods Mol. Biol. PY 2015 VL 1223 BP 17 EP 33 DI 10.1007/978-1-4939-1695-5_2 D2 10.1007/978-1-4939-1695-5 PG 17 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA BB4ON UT WOS:000343249500003 PM 25300828 ER PT S AU Sullivan, ML Quesenberry, KH AF Sullivan, Michael L. Quesenberry, Kenneth H. BE Wang, K TI Clover, Red (Trifolium pratense) SO AGROBACTERIUM PROTOCOLS, VOL 1, 3RD EDITION SE Methods in Molecular Biology LA English DT Article; Book Chapter DE Forage legume; Genetic transformation; Posttranscriptional gene silencing; Red clover; Trifolium pratense ID POLYPHENOL OXIDASE; SELECTABLE MARKERS; AGROBACTERIUM; VECTORS; REGENERATION; PLANTS; TRANSFORMATION; EXPRESSION; CULTURE; ALFALFA AB Genetic modification of plants by the insertion of transgenes can be a powerful experimental approach to answer basic questions about gene product function. This technology can also be used to make improved crop varieties for use in the field. To apply this powerful tool to red clover, an important forage legume, a population of red clover with high potential for regeneration in tissue culture has been developed. Here we provide a detailed procedure for Agrobacterium-mediated transformation of genotypes derived from this regenerable population. We have successfully used this methodology to express beta-glucuronidase (GUS) reporter genes as well as for hairpin RNA-mediated silencing of endogenous genes for polyphenol oxidase and a transferase crucial in phaselic acid accumulation. C1 [Sullivan, Michael L.] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Quesenberry, Kenneth H.] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. RP Sullivan, ML (reprint author), ARS, USDA, US Dairy Forage Res Ctr, Madison, WI 53706 USA. OI Sullivan, Michael/0000-0002-8517-4493 NR 20 TC 2 Z9 2 U1 2 U2 7 PU HUMANA PRESS INC PI TOTOWA PA 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA SN 1064-3745 BN 978-1-4939-1695-5; 978-1-4939-1694-8 J9 METHODS MOL BIOL JI Methods Mol. Biol. PY 2015 VL 1223 BP 237 EP 254 DI 10.1007/978-1-4939-1695-5_19 D2 10.1007/978-1-4939-1695-5 PG 18 WC Biochemical Research Methods; Biochemistry & Molecular Biology; Plant Sciences SC Biochemistry & Molecular Biology; Plant Sciences GA BB4ON UT WOS:000343249500020 PM 25300845 ER PT J AU Mesak, HI Bari, A Luehlfing, MS Han, F AF Mesak, Hani I. Bari, Abdullahel Luehlfing, Michael S. Han, Fei TI On modeling the advertising-operations interface under asymmetric competition SO EUROPEAN JOURNAL OF OPERATIONAL RESEARCH LA English DT Article DE Advertising endogeneity; Economic order quantity; Market-share attraction models; Sensitivity analysis; Supermodular games ID PRICE-SENSITIVE DEMAND; INVENTORY POLICIES; DYNAMIC DUOPOLY; RESEARCH DIRECTIONS; COMPARATIVE STATICS; LANCHESTER MODEL; ORDER QUANTITY; PULSING MODEL; SELLING PRICE; DECISIONS AB Using a market share attraction structure of advertising competition and following a supermodular game approach, this article demonstrates for an asymmetric oligopoly, the directional impact of changes in model parameters on the marketing controlled variables of all rivals (advertising budgets) and the operations controlled variables of all rivals (ordered quantities). Importantly, the various changes are examined analytically, empirically and numerically in both non-dominated and dominated asymmetric oligopolies. In this regard, the results indicate that firms in a dominated oligopoly (one firm of market share larger than or equal to 50%) behave differently compared to firms in a non-dominated oligopoly (each firm of market share less than 50%) in response to changes in model parameters. Furthermore, changes in model parameters are investigated in terms of their relative influential impact on a variety of equilibrium measures. In this regard, the findings indicate that for the analyzed model the marketing parameters exert much more influence on the equilibrium measures than the operations parameters. Additionally, a change in the mode of competition from non-cooperation (oligopoly) to cooperation (joint ownership) dictates that strong asymmetric firms (of favorable marketing and operations parameters) continue advertising (but at lower levels) and weak asymmetric firms (of less favorable parameters) cease advertising altogether. (C) 2014 Elsevier B.V. All rights reserved. C1 [Mesak, Hani I.] Louisiana Tech Univ, Coll Business, Dept Mkt & Anal, Ruston, LA 71272 USA. [Bari, Abdullahel] Univ Texas Tyler, Coll Business & Technol, Tyler, TX 75799 USA. [Luehlfing, Michael S.] Louisiana Tech Univ, Coll Business, Sch Accountancy, Ruston, LA 71272 USA. [Luehlfing, Michael S.] USDA, Nutrient Data Lab, Beltsville, MD 20705 USA. RP Mesak, HI (reprint author), Louisiana Tech Univ, Coll Business, Dept Mkt & Anal, POB 10318, Ruston, LA 71272 USA. EM mesak@latech.edu; abari@uttyler.edu; michaell@latech.edu; Hanfei28@gmail.com RI Han, Fei/O-1980-2015 OI Han, Fei/0000-0003-2454-4187 NR 115 TC 3 Z9 3 U1 4 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0377-2217 EI 1872-6860 J9 EUR J OPER RES JI Eur. J. Oper. Res. PD JAN 1 PY 2015 VL 240 IS 1 BP 278 EP 291 DI 10.1016/j.ejor.2014.07.009 PG 14 WC Management; Operations Research & Management Science SC Business & Economics; Operations Research & Management Science GA AR1YI UT WOS:000343380100024 ER PT J AU Lee, KM Runyon, M Herrman, TJ Phillips, R Hsieh, J AF Lee, Kyung-Min Runyon, Mick Herrman, Timothy J. Phillips, Robert Hsieh, John TI Review of Salmonella detection and identification methods: Aspects of rapid emergency response and food safety SO FOOD CONTROL LA English DT Review DE Salmonella detection method; Food emergency response; Food safety; Foodborne illness; Public health ID POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; 24-HOUR ENRICHMENT PROTOCOLS; PATHOGENIC BACTERIA; ELECTROCHEMICAL BIOSENSORS; MOLECULAR TECHNIQUES; FOODBORNE PATHOGENS; ESCHERICHIA-COLI; MULTIPLEX PCR; UNITED-STATES AB Salmonella has been recognized as a major and important foodborne pathogen for humans and animals over more than a century, causing human foodborne illness as well as high medical and economical cost. Accordingly, the effort to develop efficient and reliable Salmonella detection methods continues. This paper reviews and describes the development and application of commercially available Salmonella detection methods. These are categorized into several groups based on the principle applied: conventional culture methods, immunology-based assays, nucleic acid-based assays, miniaturized biochemical assays, and biosensors. Conventional culture methods serve as the basis in food testing laboratories despite rather laborious and time-consuming protocols. Considerable progress in rapid methods using emerging technologies yield faster answers and higher throughput of samples. This paper also shows and analyzes Salmonella test results and summarizes the features and limitations of the studies involving Salmonella detection methods developed for emergency response, mainly by food emergency response laboratories participating during recent fiscal years. The emergency response laboratories utilize Salmonella detection methods possessing properties that include simplicity, versatility, high sensitivity, good specificity, and cost efficiency. Collaboration of the food emergency response laboratories in the development of these technologies is important essentially to compare for the purpose of continually improving Salmonella detection methods. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Lee, Kyung-Min; Runyon, Mick; Herrman, Timothy J.; Hsieh, John] Texas A&M Univ Syst, Texas AgriLife Res, Off Texas State Chem, College Stn, TX 77841 USA. [Phillips, Robert] USDA, Food Safety & Inspect Serv, Athens, GA 30605 USA. RP Lee, KM (reprint author), Texas A&M Univ Syst, Texas AgriLife Res, Off Texas State Chem, College Stn, TX 77841 USA. EM kml@otsc.tamu.edu FU USDA-FSIS Microbiology Cooperative Agreement Program FX The authors acknowledge that this study was supported and made possible by funds from USDA-FSIS Microbiology Cooperative Agreement Program. NR 107 TC 20 Z9 21 U1 12 U2 166 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JAN PY 2015 VL 47 BP 264 EP 276 DI 10.1016/j.foodcont.2014.07.011 PG 13 WC Food Science & Technology SC Food Science & Technology GA AR5GI UT WOS:000343612700039 ER PT J AU Pang, YH Zhang, L Zhou, SY Yam, KL Liu, LS Sheen, S AF Pang, Yu-Hsin Zhang, Li Zhou, Siyuan Yam, Kit L. Liu, Linshu Sheen, Shiowshuh TI Growth behavior prediction of fresh catfish fillet with Pseudomonas aeruginosa under stresses of allyl isothiocyanate, temperature and modified atmosphere SO FOOD CONTROL LA English DT Article DE Modeling; Pseudomonas aeruginosa; Catfish fillet; Allyl isothiocyanate; Modified atmosphere packaging ID SHELF-LIFE; LISTERIA-MONOCYTOGENES; BACTERIAL-GROWTH; ESSENTIAL OILS; SPOILAGE; MODEL; FISH; MICROBIOLOGY; OZONE; FOOD AB Pseudomonas aeruginosa, an opportunistic pathogen which can be found on fresh catfish fillet, grows rapidly when temperature rises above 4 degrees C. The combination of allyl isothiocyanate (AIT) and modified atmosphere (MA) was applied and proved effective to retard the growth of P. aeruginosa. The objective of this research was to develop simple mathematical models to predict the growth behavior of P. aeruginosa in catfish fillet and its potential storage time (in abuse temperature condition) as a function of AIT and temperature with/without MA treatment. The antimicrobial effect of gaseous AIT (0, 18 and 36 mu L/L) on the growth of P. aeruginosa cocktail was evaluated at 8,15 and 20 degrees C. Furthermore, the effects of MA (49% CO2, 50.5% N-2 and 0.5% O-2) alone and AIT/MA combination were also investigated. These data obtained through an experimental design were used for model development. The regression models for lag phase, growth rate and "shelf life" (based on P. aeruginosa which closely related to Pseudomonas fluorescens and Pseudomonas pudita and all belong to the same RNA group used to classify the Pseudomonas at the subgenus level) were validated experimentally. Surface plot of models was also drawn to lucidly represent the interaction of AIT and temperature. The developed models may provide useful information for food industry in designing or selecting the proper packaging system with incorporation of AIT to attain the food safety with acceptable "shelf life" of fresh fish products under specified and potential abuse (with temperature deviation) distribution and storage conditions. Published by Elsevier Ltd. C1 [Pang, Yu-Hsin; Zhang, Li; Zhou, Siyuan; Yam, Kit L.] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA. [Liu, Linshu; Sheen, Shiowshuh] Agr Res Serv, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Sheen, S (reprint author), Agr Res Serv, USDA, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM shiowshuh.sheen@ars.usda.gov NR 28 TC 1 Z9 1 U1 2 U2 42 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JAN PY 2015 VL 47 BP 326 EP 333 DI 10.1016/j.foodcont.2014.07.030 PG 8 WC Food Science & Technology SC Food Science & Technology GA AR5GI UT WOS:000343612700048 ER PT J AU Busman, M Bobell, JR Maragos, CM AF Busman, Mark Bobell, John R. Maragos, Chris M. TI Determination of the aflatoxin M-1 (AFM(1)) from milk by direct analysis in real time - mass spectrometry (DART-MS) SO FOOD CONTROL LA English DT Article DE Milk; Mycotoxin; Aflatoxin; Mass spectrometry; Direct analysis in real time ID IMMUNOAFFINITY COLUMN CLEANUP; LIQUID-CHROMATOGRAPHY; AMBIENT CONDITIONS; ENZYME-IMMUNOASSAY; INFANT FORMULA; MYCOTOXINS; PRODUCTS; FOOD; EXTRACTION; FEED AB Certain fungi that grow on crops can produce aflatoxins, which are highly carcinogenic. One of these, aflatoxin B-1 can be metabolized by mammals to aflatoxin M-1, a form that retains potent carcinogenicity and which can be excreted into milk. Direct analysis in real time (DART) ionization coupled to a high resolution mass spectrometer (MS) was used for the rapid quantitative analysis of a common form of aflatoxin, AFM(1), extracted from cow milk. Sample preparation procedure and instrument parameter settings were optimized to obtain sensitive and accurate determination of AFM(1). The lowest calibration level (LCL) for aflatoxin AFM(1) was 0.1 mu g/kg. Quantitative analysis was performed with the use of matrix-matched standards employing a C-13-labeled internal standard for AFM(1). DART-MS of spiked milk extracts gave linear response over the range of 0.1-2.5 mu g/kg. Good recoveries (94.7-109.2%) and repeatabilities (RSD 13.5-9.6%) were obtained at spiking levels of 0.5 and 2.0 mu g/kg. The results of the study further demonstrate the potential of ambient ionization-MS techniques for the sensitive, convenient and rapid quantitative determination of mycotoxins from difficult matrices. Published by Elsevier Ltd. C1 [Busman, Mark; Bobell, John R.; Maragos, Chris M.] ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. RP Busman, M (reprint author), ARS, Bacterial Foodborne Pathogens & Mycol Res Unit, Natl Ctr Agr Utilizat Res, USDA, Peoria, IL 61604 USA. EM Mark.Busman@ars.usda.gov NR 39 TC 14 Z9 14 U1 9 U2 81 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JAN PY 2015 VL 47 BP 592 EP 598 DI 10.1016/j.foodcont.2014.08.003 PG 7 WC Food Science & Technology SC Food Science & Technology GA AR5GI UT WOS:000343612700083 ER PT J AU Kou, LP Luo, YG Ingram, DT Yan, SL Jurick, WM AF Kou, Liping Luo, Yaguang Ingram, David T. Yan, Shoulei Jurick, Wayne M., II TI Open-refrigerated retail display case temperature profile and its impact on product quality and microbiota of stored baby spinach SO FOOD CONTROL LA English DT Article DE Baby spinach; Temperature profile; Display case; Product quality; Retail storage ID ESCHERICHIA-COLI O157H7; STORAGE-TEMPERATURE; SHELF-LIFE; FRESH; CABINETS; ROMAINE; LETTUCE; PERFORMANCE; LEAVES; TIME AB Maintaining proper storage temperature is critical for ensuring the quality and safety of fresh-cut products. The US Food and Drug Administration Food Code recommend that packaged fresh-cut leafy green vegetables be kept no warmer than 5 C at all times to ensure food safety. Substantial temperature variations, however, within the widely used open refrigerated display cases used in retail stores are known to present the technical challenge of complying with this federal guidance for industry. This study determined the extent of the spatial and temporal temperature variations within two commercial open-refrigerated display cases under different operating conditions, and their impact on the quality and microbial growth of packaged baby spinach products. The packaged products were received within 2 d of commercial processing and temperature data loggers were placed inside-and-outside of each bag. All bags were immediately loaded in the display cases and the overall visual quality, tissue electrolyte leakage, total aerobic mesophilic bacteria and psychrotrophic bacteria were evaluated for each bag. Results from this study showed that the temperature variation in the cases was dependent on spatial location, thermostat setting, and defrost cycle interval and duration of defrost. The largest temperature differentials were found for samples located in the front and back rows of the display cases. Samples located in the front rows had the highest temperature due to heat penetration from the surrounding ambient environment, while those in the back were damaged as temperatures fell below freezing. These products received low quality scores and had higher tissue electrolyte leakage. In order to reduce the large temperature variations in the display cases, insulating foam boards were installed which significantly (P < 0.05) decreased the temperature variation by 3.5 degrees C and enabled samples in the front rows of the cases to remain less than 5 degrees C as recommended by the FDA. These results suggest that the quality and safety of packaged ready-to-eat spinach at retail will benefit from improvements in open refrigerated case design or the utilization of insulation, doors or curtains. Published by Elsevier Ltd. C1 [Kou, Liping] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China. [Kou, Liping; Luo, Yaguang; Yan, Shoulei; Jurick, Wayne M., II] USDA ARS, Food Qual Lab, Beltsville, MD 20705 USA. [Luo, Yaguang] USDA ARS, Environm Microbiol & Food Safety Lab, Beltsville, MD 20705 USA. [Ingram, David T.] US FDA, College Pk, MD 20740 USA. [Yan, Shoulei] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China. RP Luo, YG (reprint author), USDA ARS, Environm Microbiol & Food Safety Lab, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM Yaguang.Luo@ars.usda.gov FU USDA-NIFA (Specialty Crop Initiative) [2010-51181-21230]; USDA-ARS [1275-43440-004-00D] FX The authors wish to thank Dr. Roger Billingsley at Dole Fresh Vegetables (Salinas, CA, USA) for donating packaged baby spinach products, Dr. Jose Atilio de Frias for reviewing and revising the manuscript prior to its submission. This research is supported by USDA-NIFA (Specialty Crop Initiative Award No. 2010-51181-21230) and USDA-ARS (Project No. 1275-43440-004-00D). NR 22 TC 3 Z9 5 U1 15 U2 50 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0956-7135 EI 1873-7129 J9 FOOD CONTROL JI Food Control PD JAN PY 2015 VL 47 BP 686 EP 692 DI 10.1016/j.foodcont.2014.07.054 PG 7 WC Food Science & Technology SC Food Science & Technology GA AR5GI UT WOS:000343612700096 ER PT J AU Cole, MR Rose, I Chung, YJ Eggleston, G AF Cole, Marsha R. Rose, Ingrid Chung, Yoo Jin Eggleston, Gillian TI A structured approach to target starch solubilisation and hydrolysis for the sugarcane industry SO FOOD CHEMISTRY LA English DT Article DE Soluble starch; Insoluble starch; Swollen starch; Amylases; Activity; Hydrolysis; Sugarcane processing ID ALPHA-AMYLASE APPLICATION; THERMOSTABLE ENZYMES; APPLE JUICE; RAW; GELATINIZATION; MANUFACTURE; WATER; DENATURATION; OPTIMIZATION; IONS AB In sugarcane processing, starch is considered an impurity that negatively affects processing and reduces the quality of the sugar end-product. In the last decade, there has been a general world-wide increase in starch concentrations in sugarcane. Industrial alpha-amylases have been used for many years to mitigate issues arising from starch in the sugarcane industry. Mixed results have prompted further studies of the behaviour of different physical forms of starch and their interactions with alpha-amylases during processing. By using corn starch as a reference in model juices and syrups, processing parameters, activities, and hydrolysis of insoluble, swollen, and soluble starch forms were evaluated for two commercial alpha-amylases with high (HT) and intermediate (IT) temperature stability, respectively. The ability of starch to solubilise across a sugarcane factory is largely limited by increased Brix values. Optimum target locations and conditions for the application of alpha-amylases in sugarcane processing are discussed in detail. Published by Elsevier Ltd. C1 [Cole, Marsha R.; Chung, Yoo Jin; Eggleston, Gillian] ARS, So Reg Res Ctr, USDA, New Orleans, LA USA. [Rose, Ingrid] Agro Paris Tech, Dept Food Sci, Paris, France. RP Cole, MR (reprint author), 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM Marsha.Cole@ars.usda.gov NR 37 TC 1 Z9 1 U1 0 U2 74 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JAN 1 PY 2015 VL 166 BP 165 EP 172 DI 10.1016/j.foodchem.2014.05.151 PG 8 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AQ2XV UT WOS:000342654200023 PM 25053042 ER PT J AU Chung, SY Reed, S AF Chung, Si-Yin Reed, Shawndrika TI IgE binding to peanut allergens is inhibited by combined D-aspartic and D-glutamic acids SO FOOD CHEMISTRY LA English DT Article DE D-Amino acids; D-Glutamic acid; D-Aspartic acid; Peanut allergens; Ara h 1 and Ara h 2; IgE antibodies ID D-AMINO ACIDS; IMMUNOGLOBULIN-E; ACTIVATION; ANTIBODIES; REMOVAL; LIGANDS; LYSINE; HELIX AB The objective of this study was to determine if a-amino acids (D-aas) bind and inhibit immunoglobulin E (IgE) binding to peanut allergens. D-aas such as D-Asp (aspartic acid), D-Glu (glutamic acid), combined D-[Asp/Glu] and others were each prepared in a cocktail of 9 other D-aas, along with L-amino acids (L-aas) and controls. Each sample was mixed with a pooled plasma from peanut-allergic donors, and tested by ELISA (enzyme-linked immunosorbent assay) and Western blots for IgE binding to peanut allergens. Results showed that D-[Asp/Glul (4 mg/ml) inhibited IgE binding (75%) while D-Glu, D-Asp and other D-aas had no inhibitory effect. A higher inhibition was seen with D-[Asp/Glu] than with L-[Asp/Glu]. We concluded that IgE was specific for D-[Asp/Glu], not D-Asp or D-Glu, and that D-[Asp/Glu] was more reactive than was L-[Asp/Glu] in IgE inhibition. The finding indicates that D-[Asp/Glu] may have the potential for removing IgE or reducing IgE binding to peanut allergens in vitro. Published by Elsevier Ltd. C1 [Chung, Si-Yin; Reed, Shawndrika] USDA ARS, So Reg Res Ctr, New Orleans, LA 70124 USA. RP Chung, SY (reprint author), USDA ARS, So Reg Res Ctr, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA. EM siyin.chung@ars.usda.gov FU U.S. government FX The author thanks Ashley Guillory for technical support. This study was funded by the U.S. government. NR 31 TC 1 Z9 1 U1 2 U2 37 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JAN 1 PY 2015 VL 166 BP 248 EP 253 DI 10.1016/j.foodchem.2014.06.004 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AQ2XV UT WOS:000342654200033 PM 25053052 ER PT J AU Banuelos, GS Arroyo, I Pickering, IJ Yang, SI Freeman, JL AF Banuelos, Gary S. Arroyo, Irvin Pickering, Ingrid J. Yang, Soo In Freeman, John L. TI Selenium biofortification of broccoli and carrots grown in soil amended with Se-enriched hyperaccumulator Stanleya pinnata SO FOOD CHEMISTRY LA English DT Article DE Biofortification; Selenium; Stanleya pinnata ID RAY-ABSORPTION SPECTROSCOPY; CANCER PREVENTION; MOLECULAR-MECHANISMS; COLON-CANCER; HUMAN HEALTH; SPECIATION; SUPPLEMENTATION; ACCUMULATION; PLANTS; PHYTOREMEDIATION AB Amending soils with Se-hyperaccumulator plant derived sources of selenium (Se) may be useful for increasing the Se content in food crops in Se-deficient regions of the world. In this study we evaluated total Se and the different chemical species of Se in broccoli and carrots grown in soils amended with ground shoots of the Se-hyperaccumulator Stanleya pinnata. With increasing application rates of S. pinnata, total plant Se concentrations increased to nutritionally ideal levels inside edible parts. Selenium compounds in aqueous extracts were analyzed by SAX-HPLC-ICPMS and identified as a variety of mainly organic-Se forms. Together with bulk Se K-edge X-ray absorption near-edge structure (XANES) analysis performed on broccoli florets, carrot roots and shoots, dried ground S. pinnata, and the amended soil at post-plant, we demonstrate that Se-enriched S. pinnata is valuable as a soil amendment for enriching broccoli and carrots with healthful forms of organic-Se. Published by Elsevier Ltd. C1 [Banuelos, Gary S.; Arroyo, Irvin] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. [Pickering, Ingrid J.; Yang, Soo In] Univ Saskatchewan, Dept Geol Sci, Saskatoon, SK S7N 5E2, Canada. [Freeman, John L.] Calif State Univ Fresno, Dept Biol, Fresno, CA 93740 USA. [Freeman, John L.] Intrinsyx Technol Corp, Space Biosci Div, NASA, Ames Res Ctr, Moffett Field, CA 94035 USA. RP Banuelos, GS (reprint author), ARS, USDA, San Joaquin Valley Agr Sci Ctr, 9611 South Riverbend Ave, Parlier, CA 93648 USA. EM gary.banuelos@ars.usda.gov; john.l.freeman@nasa.gov RI Pickering, Ingrid/A-4547-2013 NR 44 TC 16 Z9 19 U1 7 U2 140 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JAN 1 PY 2015 VL 166 BP 603 EP 608 DI 10.1016/j.foodchem.2014.06.071 PG 6 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AQ2XV UT WOS:000342654200081 PM 25053099 ER PT J AU Lee, J AF Lee, Jungmin TI Sorbitol, Rubus fruit, and misconception SO FOOD CHEMISTRY LA English DT Review DE Sugar alcohol; Sugar; Rosaceae; Polyol; Carbohydrate ID PERFORMANCE LIQUID-CHROMATOGRAPHY; IRRITABLE-BOWEL-SYNDROME; RED RASPBERRY JUICE; PRUNUS-AVIUM L.; SUGAR-ALCOHOLS; NONVOLATILE ACID; ORGANIC-ACIDS; HIGHER-PLANTS; HUMAN HEALTH; APPLE AB It is unclear how the misunderstanding that Rubus fruits (e.g., blackberries, raspberries) are high in sugar alcohol began, or when it started circulating in the United States. In reality, they contain little sugar alcohol. Numerous research groups have reported zero detectable amounts of sugar alcohol in fully ripe Rubus fruit, with the exception of three out of 82 Rubus fruit samples (cloudberry 0.01 g/100 g, red raspberry 0.03 g/100 g, and blackberry 4.8 g/100 g*; *highly unusual as 73 other blackberry samples contained no detectable sorbitol). Past findings on simple carbohydrate composition of Rubus fruit, other commonly consumed Rosaceae fruit, and additional fruits (24 genera and species) are summarised. We are hopeful that this review will clarify Rosaceae fruit sugar alcohol concentrations and individual sugar composition; examples of non-Rosaceae fruit and prepared foods containing sugar alcohol are included for comparison. A brief summary of sugar alcohol and health will also be presented. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). C1 ARS, USDA, Parma, ID 83660 USA. RP Lee, J (reprint author), ARS, USDA, HCRU Worksite,29603 U I Ln, Parma, ID 83660 USA. EM jlee@uidaho.edu RI Lee, Jungmin/G-6555-2013 OI Lee, Jungmin/0000-0002-8660-9444 FU USDA-Agricultural Research Service (ARS) CRIS [5358-21000-047-00D]; Specialty Crop Research Initiative (SCRI) from USDA-National Institute of Food and Agriculture (NIFA) [2011-51181-30676] FX This project was funded by USDA-Agricultural Research Service (ARS) CRIS number 5358-21000-047-00D and a Specialty Crop Research Initiative (SCRI) Grant number 2011-51181-30676 from USDA-National Institute of Food and Agriculture (NIFA). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 67 TC 2 Z9 2 U1 10 U2 48 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD JAN 1 PY 2015 VL 166 BP 616 EP 622 DI 10.1016/j.foodchem.2014.06.073 PG 7 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AQ2XV UT WOS:000342654200083 PM 25053101 ER PT J AU Broadhurst, CL Chaney, RL Davis, AP Cox, A Kumar, K Reeves, RD Green, CE AF Broadhurst, C. Leigh Chaney, Rufus L. Davis, Allen P. Cox, Albert Kumar, Kuldip Reeves, Roger D. Green, Carrie E. TI Growth and Cadmium Phytoextraction by Swiss Chard, Maize, Rice, Noccaea caerulescens, and Alyssum murale in Ph Adjusted Biosolids Amended Soils SO INTERNATIONAL JOURNAL OF PHYTOREMEDIATION LA English DT Article DE Noccaea caerulescens; Swiss chard; maize; corn; rice; phytoremediation; phytoextraction cadmium; zinc; biosolids ID METAL-CONTAMINATED SITES; TURGIDUM-DURUM L.; THLASPI-CAERULESCENS; SEWAGE-SLUDGE; SEDUM-ALFREDII; POLLUTED SOILS; INBRED LINES; IN-SITU; CD; ZINC AB Past applications of biosolids to soils at some locations added higher Cd levels than presently permitted. Cadmium phytoextraction would alleviate current land use constraints. Unamended farm soil, and biosolids amended farm and mine soils were obtained from a Fulton Co., IL biosolids management facility. Soils contained 0.16, 22.8, 45.3 mg Cd kg(-1) and 43.1, 482, 812 mg Zn kg(-1) respectively with initial pH 6.0, 6.1, 6.4. In greenhouse studies, Swiss chard (Beta vulgaris var. cicla), a Cd-accumulator maize (inbred B37 Zea mays) and a southern France Cd-hyperaccumulator genotype of Noccaea caerulescens were tested for Cd accumulation and phytoextraction. Soil pH was adjusted from similar to 5.5-7.0. Additionally 100 rice (Oryza sativa) genotypes and the Ni-hyperaccumulator Alyssum murale were screened for potential phytoextraction use. Chard suffered phytotoxicity at low pH and accumulated up to 90 mg Cd kg(-1) on the biosolids amended mine soil. The maize inbred accumulated up to 45 mg Cd kg(-1) with only mild phytotoxicity symptoms during early growth at pH > 6.0. N. caerulescens did not exhibit phytotoxicity symptoms at any pH, and accumulated up to 235 mg Cd kg(-1) in 3 months. Reharvested N. caerulescens accumulated up to 900 mg Cd kg(-1) after 10 months. Neither Alyssum nor 90% of rice genotypes survived acceptably. Both N. caerulescens and B37 maize show promise for Cd phytoextraction in IL and require field evaluation; both plants could be utilized for nearly continuous Cd removal. Other maize inbreds may offer higher Cd phytoextraction at lower pH, and mono-cross hybrids higher shoot biomass yields. Further, maize grown only for biomass Cd maximum removal could be double-cropped. C1 [Broadhurst, C. Leigh; Chaney, Rufus L.; Green, Carrie E.] USDA ARS, Environm Management & Byprod Utilizat Lab, Beltsville, MD 20705 USA. [Broadhurst, C. Leigh; Davis, Allen P.] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA. [Cox, Albert; Kumar, Kuldip] Metropolitan Water Reclamat Dist Greater Chicag, Cicero, IL USA. [Reeves, Roger D.] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia. RP Broadhurst, CL (reprint author), USDA ARS, Environm Management & Byprod Utilizat Lab, 10300 Baltimore Blvd,Bldg 007, Beltsville, MD 20705 USA. EM leigh.broadhurst@ars.usda.gov RI DAVIS, ALLEN/F-1066-2017 OI DAVIS, ALLEN/0000-0001-7818-1890 NR 51 TC 5 Z9 5 U1 4 U2 173 PU TAYLOR & FRANCIS INC PI PHILADELPHIA PA 530 CHESTNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA SN 1522-6514 EI 1549-7879 J9 INT J PHYTOREMEDIAT JI Int. J. Phytoremediat. PY 2015 VL 17 IS 1 BP 25 EP 39 DI 10.1080/15226514.2013.828015 PG 15 WC Environmental Sciences SC Environmental Sciences & Ecology GA AO0TG UT WOS:000341024400004 PM 25174422 ER PT J AU Irwin, P Nguyen, L He, YP Paoli, G Gehring, A Chen, CY AF Irwin, Peter Ly Nguyen He, Yiping Paoli, George Gehring, Andrew Chen, Chin-Yi TI The near-quantitative sampling of genomic DNA from various food-borne Eubacteria SO BMC MICROBIOLOGY LA English DT Article ID 16S RIBOSOMAL-RNA; REAL-TIME PCR; POLYMERASE-CHAIN-REACTION; BACTERIAL-DNA; BROAD-RANGE; COMMUNITY COMPOSITION; CAMPYLOBACTER-JEJUNI; MICROORGANISMS; EXTRACTION; COLI AB Background: The disruption of the bacterial cell wall plays an important part in achieving quantitative extraction of DNA from Eubacteria essential for accurate analyses of genetic material recovered from environmental samples. Results: In this work we have tested a dozen commercial bacterial genomic DNA extraction methodologies on an average of 7.70 x 106 (+/- 9.05%), 4.77 x 108 (+/- 31.0%), and 5.93 x 108 (+/- 4.69%) colony forming units (CFU) associated with 3 cultures (n = 3) each of Brochothrix thermosphacta (Bt; Gram-positive), Shigella sonnei (Ss; Gram-negative), and Escherichia coli O79 (Ec; Gram-negative). We have utilized real-time PCR (qPCR) quantification with two specific sets of primers associated with the 16S rRNA " gene" to determine the number of copies CFU-1 by comparing the unknown target DNA qPCR results with standards for each primer set. Based upon statistical analyses of our results, we determined that the Agencourt Genfind v2, High Pure PCR Template Prep Kit, and Omnilyse methods consistently provided the best yield of genomic DNA ranging from 141 to 934, 8 to 21, and 16 to 27 16S rDNA copies CFU-1 for Bt, Ss, and Ec. If one assumes 6-7 copies of the 16S rRNA gene per genome, between 1 and 3 genomes per actively dividing cell and = 100 cells CFU-1 for Bt (found to be a reasonable assumption using an optical method expounded upon herein) or between 1 and 2 cells CFU-1 for either Ss or Ec, then the Omnilyse procedure provided nearly quantitative extraction of genomic DNA from these isolates (934 19.9 copies CFU-1 for Bt; 20.8 +/- 2.68 copies CFU-1 for Ss; 26.9 +/- 3.39 copies CFU-1 for Ec). The Agencourt, High Pure, and Omnilyse technologies were subsequently assessed using 5 additional Gram-positive and 10 Gram-negative foodborne isolates (n = 3) using a set of " universal" 16S rDNA primers. Conclusion: Overall, the most notable DNA extraction method was found to be the Omnilyse procedure which is a " bead blender" technology involving high frequency agitation in the presence of zirconium silicate beads. C1 [Irwin, Peter; Ly Nguyen; He, Yiping; Paoli, George; Gehring, Andrew; Chen, Chin-Yi] ARS, USDA, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. RP Irwin, P (reprint author), ARS, USDA, Mol Characterizat Foodborne Pathogens Res Unit, Eastern Reg Res Ctr, 600 E Mermaid Lane, Wyndmoor, PA 19038 USA. EM Peter.Irwin@ars.usda.gov FU Current Research Information System (CRIS) [8072-42000-067-00D] FX All funding was from ARS base funds associated with Current Research Information System (CRIS) Project Number 8072-42000-067-00D (Microbial Communities and Interactions and their Impact on Food Safety). NR 39 TC 3 Z9 3 U1 1 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2180 J9 BMC MICROBIOL JI BMC Microbiol. PD DEC 31 PY 2014 VL 14 AR 326 DI 10.1186/s12866-014-0326-z PG 17 WC Microbiology SC Microbiology GA AZ4HR UT WOS:000348182800001 PM 25551371 ER PT J AU Silveira, JQ Cesar, TB Manthey, JA Baldwin, EA Bai, JH Raithore, S AF Silveira, Jacqueline Q. Cesar, Thais B. Manthey, John A. Baldwin, Elizabeth A. Bai, Jinhe Raithore, Smita TI Pharmacokinetics of Flavanone Glycosides after Ingestion of Single Doses of Fresh-Squeezed Orange Juice versus Commercially Processed Orange Juice in Healthy Humans SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE bioavailability; pharmacokinetics; hesperidin; narirutin; metabolites; juice cloud ID SPONTANEOUSLY HYPERTENSIVE-RATS; CITRUS FLAVONOID HESPERIDIN; SERUM TRIGLYCERIDE LEVEL; GLUCOSYL HESPERIDIN; POLYMETHOXYLATED FLAVONES; DENSITY-LIPOPROTEIN; LIPID-METABOLISM; PHENOLIC-ACIDS; BONE LOSS; IN-VITRO C1 [Silveira, Jacqueline Q.; Cesar, Thais B.] Sao Paulo State Univ UNESP, Fac Pharmaceut Sci, Food & Nutr Dept, BR-14801902 Araraquara, SP, Brazil. [Manthey, John A.; Baldwin, Elizabeth A.; Bai, Jinhe; Raithore, Smita] ARS, US Hort Res Lab, USDA, Ft Pierce, FL 34945 USA. RP Manthey, JA (reprint author), ARS, US Hort Res Lab, USDA, 2001 South Rock Rd, Ft Pierce, FL 34945 USA. EM john.manthey@ars.usda.gov RI Cesar, Thais/I-4540-2013 OI Cesar, Thais/0000-0001-7878-7075 FU Brazilian agency CAPES [Proc. BEX 18493/12-2] FX The authors are grateful to the Brazilian agency CAPES for grant of scholarship to Jacqueline Silveira (Proc. BEX 18493/12-2), and to Citrosuco S/A (Brazil) for supplying oranges and orange juice for this study. NR 48 TC 6 Z9 6 U1 5 U2 25 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 31 PY 2014 VL 62 IS 52 BP 12576 EP 12584 DI 10.1021/jf5038163 PG 9 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AY2RS UT WOS:000347437500014 PM 25495754 ER PT J AU Zhang, YZ Du, WX Fregevu, C Kothary, MH Harden, L McHugh, TH AF Zhang, Yuzhu Du, Wen-Xian Fregevu, Cecile Kothary, Mahendra H. Harden, Leslie McHugh, Tara H. TI Expression, Purification, and Characterization of Almond (Prunus dulcis) Allergen Pru du 4 SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE food allergy; sumo; tree nut allergy; stability; almond; allergen ID FOOD ALLERGY; PROFILIN; PROTEIN; SOLUBILITY; STABILITY; CLONING C1 [Zhang, Yuzhu; Du, Wen-Xian; Fregevu, Cecile; Harden, Leslie; McHugh, Tara H.] ARS, USDA, Pacific West Area, Western Reg Res Ctr, Albany, CA 94710 USA. [Kothary, Mahendra H.] US FDA, Ctr Food Safety & Appl Nutr, Laurel, MD 20708 USA. RP Zhang, YZ (reprint author), ARS, USDA, Pacific West Area, Western Reg Res Ctr, 800 Buchanan St, Albany, CA 94710 USA. EM yuzhu.zhang@ars.usda.gov NR 26 TC 3 Z9 3 U1 3 U2 18 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 31 PY 2014 VL 62 IS 52 BP 12695 EP 12700 DI 10.1021/jf5045102 PG 6 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AY2RS UT WOS:000347437500027 PM 25437489 ER PT J AU Chopra, R Burow, G Farmer, A Mudge, J Simpson, CE Burow, MD AF Chopra, Ratan Burow, Gloria Farmer, Andrew Mudge, Joann Simpson, Charles E. Burow, Mark D. TI Comparisons of De Novo Transcriptome Assemblers in Diploid and Polyploid Species Using Peanut (Arachis spp.) RNA-Seq Data SO PLOS ONE LA English DT Article ID BURROWS-WHEELER TRANSFORM; CULTIVATED PEANUT; READ ALIGNMENT; HYPOGAEA L.; GENOME; GENERATION; DISCOVERY; RELATIVES; PROGRAM; MARKERS AB The narrow genetic base and limited genetic information on Arachis species have hindered the process of marker-assisted selection of peanut cultivars. However, recent developments in sequencing technologies have expanded opportunities to exploit genetic resources, and at lower cost. To use the genetic information for Arachis species available at the transcriptome level, it is important to have a good quality reference transcriptome. The available Tifrunner 454 FLEX transcriptome sequences have an assembly with 37,000 contigs and low N50 values of 500-751bp. Therefore, we generated de novo transcriptome assemblies, with about 38 million reads in the tetraploid cultivar OLin, and 16 million reads in each of the diploids, A. duranensis K38901 and A. ipaensis KGBSPSc30076 using three different de novo assemblers, Trinity, SOAPdenovo-Trans and TransAByss. All these assemblers can use single kmer analysis, and the latter two also permit multiple kmer analysis. Assemblies generated for all three samples had N50 values ranging from 1278-1641 bp in Arachis hypogaea (AABB), 1401-1492 bp in Arachis duranensis (AA), and 1107-1342 bp in Arachis ipaensis (BB). Comparison with legume ESTs and protein databases suggests that assemblies generated had more than 40% full length transcripts with good continuity. Also, on mapping the raw reads to each of the assemblies generated, Trinity had a high success rate in assembling sequences compared to both TransAByss and SOAPdenovo-Trans. De novo assembly of OLin had a greater number of contigs (67,098) and longer contig length (N50=1,641) compared to the Tifrunner TSA. Despite having shorter read length (2x50) than the Tifrunner 454FLEX TSA, de novo assembly of OLin proved superior in comparison. Assemblies generated to represent different genome combinations may serve as a valuable resource for the peanut research community. C1 [Chopra, Ratan; Burow, Mark D.] Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. [Burow, Gloria] ARS, USDA, CSRL, Lubbock, TX 79415 USA. [Farmer, Andrew; Mudge, Joann] Natl Ctr Genome Resources, Santa Fe, NM 87505 USA. [Simpson, Charles E.] Texas A&M AgriLife Res, Stephenville, TX 76401 USA. [Burow, Mark D.] Texas A&M AgriLife Res, Lubbock, TX 79403 USA. RP Burow, MD (reprint author), Texas Tech Univ, Dept Plant & Soil Sci, Lubbock, TX 79409 USA. EM mburow@tamu.edu OI CHOPRA, RATAN/0000-0003-2088-3341 FU Texas Peanut Producers Board; National Peanut Board [332/TX-99/1139, 332/TX-99/1213]; CES; Peanut Foundation [04-810-08]; Ogallala Aquifer Initiative [IPM12.06]; United States Department of Agriculture/National Institute of Food and Agriculture Hatch Act [TEX08835] FX This work was supported by awards from the Texas Peanut Producers Board (http://www.texaspeanutboard.com) award CY2008-Burow-TTU-Development to MDB and CES, and 2009-TTU-Burow-Genotyping to MDB, National Peanut Board (http://nationalpeanutboard.org) grant #332/TX-99/1139 to MDB, and #332/TX-99/1213 to MDB and CES, Peanut Foundation (http://peanutfoundation.org) grant 04-810-08 to MDB, Ogallala Aquifer Initiative (http://ogallala.ars.usda.gov) award IPM12.06 to MDB, and United States Department of Agriculture/National Institute of Food and Agriculture Hatch Act (http://www.csrees.usda.gov/business/awards/formula/hatch.html) award TEX08835 to MDB funding. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 11 Z9 11 U1 4 U2 29 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 31 PY 2014 VL 9 IS 12 AR e115055 DI 10.1371/journal.pone.0115055 PG 16 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX7UC UT WOS:000347119100026 PM 25551607 ER PT J AU Danies, G Myers, K Mideros, MF Restrepo, S Martin, FN Cooke, DEL Smart, CD Ristaino, JB Seaman, AJ Gugino, BK Grunwald, NJ Fry, WE AF Danies, Giovanna Myers, Kevin Mideros, Maria F. Restrepo, Silvia Martin, Frank N. Cooke, David E. L. Smart, Christine D. Ristaino, Jean B. Seaman, Abby J. Gugino, Beth K. Gruenwald, Niklaus J. Fry, William E. TI An Ephemeral Sexual Population of Phytophthora infestans in the Northeastern United States and Canada SO PLOS ONE LA English DT Article ID MULTILOCUS GENOTYPE DATA; RECENT CLONAL LINEAGES; CENTRAL MEXICO; LATE BLIGHT; DNA POLYMORPHISM; GENOME SEQUENCES; TOLUCA VALLEY; POTATO; OOSPORES; MARKERS AB Phytophthora infestans, the causal agent of late blight disease, has been reported in North America since the mid-nineteenth century. In the United States the lack of or very limited sexual reproduction has resulted in largely clonal populations of P. infestans. In 2010 and 2011, but not in 2012 or 2013, 20 rare and diverse genotypes of P. infestans were detected in a region that centered around central New York State. The ratio of A1 to A2 mating types among these genotypes was close to the 50: 50 ratio expected for sexual recombination. These genotypes were diverse at the glucose-6-phosphate isomerase locus, differed in their microsatellite profiles, showed different banding patterns in a restriction fragment length polymorphism assay using a moderately repetitive and highly polymorphic probe (RG57), were polymorphic for four different nuclear genes and differed in their sensitivity to the systemic fungicide mefenoxam. The null hypothesis of linkage equilibrium was not rejected, which suggests the population could be sexual. These new genotypes were monomorphic in their mitochondrial haplotype that was the same as US-22. Through parentage exclusion testing using microsatellite data and sequences of four nuclear genes, recent dominant lineages US-8, US-11, US-23, and US-24 were excluded as possible parents for these genotypes. Further analyses indicated that US-22 could not be eliminated as a possible parent for 14 of the 20 genotypes. We conclude that US-22 could be a parent of some, but not all, of the new genotypes found in 2010 and 2011. There were at least two other parents for this population and the genotypic characteristics of the other parents were identified. C1 [Danies, Giovanna; Myers, Kevin; Fry, William E.] Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY 14850 USA. [Mideros, Maria F.; Restrepo, Silvia] Univ Los Andes, Dept Biol Sci, Bogota, Colombia. [Martin, Frank N.] ARS, USDA, Salinas, CA USA. [Cooke, David E. L.] Invergowrie, James Hutton Inst, Dundee, Scotland. [Smart, Christine D.] Cornell Univ, Dept Plant Pathol & Plant Microbe Biol, Geneva, NY USA. [Ristaino, Jean B.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Seaman, Abby J.] Cornell Cooperat Extens, New York State Integrated Pest Management Program, Geneva, NY USA. [Gugino, Beth K.] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 USA. [Gruenwald, Niklaus J.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. [Gruenwald, Niklaus J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Gruenwald, Niklaus J.] Oregon State Univ, Ctr Genome Biol & Biocomp, Corvallis, OR 97331 USA. RP Fry, WE (reprint author), Cornell Univ, Sch Integrat Plant Sci, Plant Pathol & Plant Microbe Biol Sect, Ithaca, NY 14850 USA. EM wef1@cornell.edu RI Cooke, David/D-1933-2012; Grunwald, Niklaus/K-6041-2013 OI Cooke, David/0000-0002-9154-7954; Grunwald, Niklaus/0000-0003-1656-7602 FU Agriculture and Food Research Initiative Competitive Grants Program from the USDA National Institute of Food and Agriculture [2011-68004-30154]; USDA Sustainable Agriculture Research and Education [SARE GNE13-056]; College of Agriculture and Life Sciences at Cornell University FX This work was supported by the Agriculture and Food Research Initiative Competitive Grants Program Grant 2011-68004-30154 from the USDA National Institute of Food and Agriculture, by the USDA Sustainable Agriculture Research and Education (SARE GNE13-056), and by the College of Agriculture and Life Sciences at Cornell University. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 52 TC 6 Z9 7 U1 1 U2 36 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 31 PY 2014 VL 9 IS 12 AR e116354 DI 10.1371/journal.pone.0116354 PG 21 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX7UC UT WOS:000347119100133 PM 25551215 ER PT J AU Rodriguez-Bonilla, L Cuevas, HE Montero-Rojas, M Bird-Pico, F Luciano-Rosario, D Siritunga, D AF Rodriguez-Bonilla, Lorraine Cuevas, Hugo E. Montero-Rojas, Milly Bird-Pico, Fernando Luciano-Rosario, Dianiris Siritunga, Dimuth TI Assessment of Genetic Diversity of Sweet Potato in Puerto Rico SO PLOS ONE LA English DT Article ID SEQUENCE REPEAT MARKERS; BATATAS L. LAM.; POPULATION-STRUCTURE; INTERACTIVE TREE; IPOMOEA; DISPERSAL; ORIGIN; MICROSATELLITES; POLYMORPHISMS; ANNOTATION AB Sweet potato (Ipomoea batatas L.) is the seventh most important food crop due to its distinct advantages, such as adaptability to different environmental conditions and high nutritional value. Assessing the genetic diversity of this important crop is necessary due to the constant increase of demand for food and the need for conservation of agricultural and genetic resources. In Puerto Rico (PR), the genetic diversity of sweet potato has been poorly understood, although it has been part of the diet since Pre-Columbus time. Thus, 137 landraces from different localities around PR were collected and subjected to a genetic diversity analysis using 23 SSR-markers. In addition, 8 accessions from a collection grown in Gurabo, PR at the Agricultural Experimental Station (GAES), 10 US commercial cultivars and 12 Puerto Rican accessions from the USDA repository collection were included in this assessment. The results of the analysis of the 23 loci showed 255 alleles in the 167 samples. Observed heterozygosity was high across populations (0.71) while measurements of total heterozygosity revealed a large genetic diversity throughout the population and within populations. UPGMA clustering method revealed two main clusters. Cluster 1 contained 12 PR accessions from the USDA repository collection, while cluster 2 consisted of PR landraces, US commercial cultivars and the PR accessions from GAES. Population structure analysis grouped PR landraces in five groups including four US commercial cultivars. Our study shows the presence of a high level of genetic diversity of sweet potato across PR which can be related to the genetic makeup of sweet potato, human intervention and out-crossing nature of the plant. The history of domestication and dispersal of sweet potato in the Caribbean and the high levels of genetic diversity found through this study makes sweet potato an invaluable resource that needs to be protected and further studied. C1 [Rodriguez-Bonilla, Lorraine; Montero-Rojas, Milly; Bird-Pico, Fernando; Luciano-Rosario, Dianiris; Siritunga, Dimuth] Univ Puerto Rico, Dept Biol, Mayaguez, PR 00680 USA. [Cuevas, Hugo E.] ARS, Trop Agr Res Stn, USDA, Mayaguez, PR USA. RP Siritunga, D (reprint author), Univ Puerto Rico, Dept Biol, Mayaguez, PR 00680 USA. EM Dimuth.siritunga@upr.edu NR 32 TC 4 Z9 4 U1 1 U2 33 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 31 PY 2014 VL 9 IS 12 AR e116184 DI 10.1371/journal.pone.0116184 PG 14 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX7UC UT WOS:000347119100106 PM 25551388 ER PT J AU Liu, JJ Sniezko, RA Sturrock, RN Chen, H AF Liu, Jun-Jun Sniezko, Richard A. Sturrock, Rona N. Chen, Hao TI Western white pine SNP discovery and high-throughput genotyping for breeding and conservation applications SO BMC PLANT BIOLOGY LA English DT Article DE Five-needle pine; Genotyping array; Linkage disequilibrium; Marker-based selection; Pedigree reconstruction ID SINGLE-NUCLEOTIDE POLYMORPHISMS; RNA-SEQ; CRONARTIUM-RIBICOLA; BLISTER RUST; LINKAGE DISEQUILIBRIUM; TRANSCRIPTOME ANALYSIS; POPULATION-STRUCTURE; GENE-EXPRESSION; HOST-RESISTANCE; FOREST TREES AB Background: Western white pine (WWP, Pinus monticola Douglas ex D. Don) is of high interest in forest breeding and conservation because of its high susceptibility to the invasive disease white pine blister rust (WPBR, caused by the fungus Cronartium ribicola J. C. Fisch). However, WWP lacks genomic resource development and is evolutionarily far away from plants with available draft genome sequences. Here we report a single nucleotide polymorphism (SNP) study by bulked segregation-based RNA-Seq analysis. Results: A collection of resistance germplasm was used for construction of cDNA libraries and SNP genotyping. Approximately 36-89 million 2 x 100-bp reads were obtained per library and de-novo assembly generated the first shoot-tip reference transcriptome containing a total of 54,661 unique transcripts. Bioinformatic SNP detection identified > 100,000 high quality SNPs in three expressed candidate gene groups: Pinus highly conserved genes (HCGs), differential expressed genes (DEGs) in plant defense response, and resistance gene analogs (RGAs). To estimate efficiency of in-silico SNP discovery, genotyping assay was developed by using Sequenom iPlex and it unveiled SNP success rates from 40.1% to 61.1%. SNP clustering analyses consistently revealed distinct populations, each composed of multiple full-sib seed families by parentage assignment in the WWP germplasm collection. Linkage disequilibrium (LD) analysis identified six genes in significant association with major gene (Cr2) resistance, including three RGAs (two NBS-LRR genes and one receptor-like protein kinase -RLK gene), two HCGs, and one DEG. At least one SNP locus provided an excellent marker for Cr2 selection across P. monticola populations. Conclusions: The WWP shoot tip transcriptome and those validated SNP markers provide novel genomic resources for genetic, evolutionary and ecological studies. SNP loci of those candidate genes associated with resistant phenotypes can be used as positional and functional variation sites for further characterization of WWP major gene resistance against C. ribicola. Our results demonstrate that integration of RNA-seq-based transcriptome analysis and high-throughput genotyping is an effective approach for discovery of a large number of nucleotide variations and for identification of functional gene variants associated with adaptive traits in a non-model species. C1 [Liu, Jun-Jun; Sturrock, Rona N.; Chen, Hao] Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, Victoria, BC V8Z 1M5, Canada. [Sniezko, Richard A.] US Forest Serv, USDA, Dorena Genet Resource Ctr, Cottage Grove, OR 97424 USA. RP Liu, JJ (reprint author), Nat Resources Canada, Canadian Forest Serv, Pacific Forestry Ctr, 506 West Burnside Rd, Victoria, BC V8Z 1M5, Canada. EM Jun-Jun.Liu@NRCan-RNCan.gc.ca FU CFS; CFS-GRDI fund award FX We are grateful to Aimin Guan and Gary Zhang for bioinformatic programming, Kristian Arndt for database management, Arezoo Zamany for sample collection, Angelia Kegley and colleagues at DGRC for resistant trait assessment, and Grace Sumampong and Danelle Chan for genomic DNA extraction. This research was supported partly by CFS and the CFS-GRDI fund award to J-JL. NR 61 TC 5 Z9 5 U1 7 U2 64 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD DEC 30 PY 2014 VL 14 AR 380 DI 10.1186/s12870-014-0380-6 PG 13 WC Plant Sciences SC Plant Sciences GA CA0IZ UT WOS:000348602700002 PM 25547170 ER PT J AU Zila, CT Ogut, F Romay, MC Gardner, CA Buckler, ES Holland, JB AF Zila, Charles T. Ogut, Funda Romay, Maria C. Gardner, Candice A. Buckler, Edward S. Holland, James B. TI Genome-wide association study of Fusarium ear rot disease in the USA maize inbred line collection SO BMC PLANT BIOLOGY LA English DT Article DE Association analysis; Disease resistance; Genomic selection; Maize; Quantitative trait ID FALSE DISCOVERY RATE; FUMONISIN CONTAMINATION; KERNEL INFECTION; RESISTANCE; CORN; VERTICILLIOIDES; MONILIFORME; POPULATIONS; GENES; IDENTIFICATION AB Background: Resistance to Fusarium ear rot of maize is a quantitative and complex trait. Marker-trait associations to date have had small additive effects and were inconsistent between previous studies, likely due to the combined effects of genetic heterogeneity and low power of detection of many small effect variants. The complexity of inheritance of resistance hinders the use marker-assisted selection for ear rot resistance. Results: We conducted a genome-wide association study (GWAS) for Fusarium ear rot resistance in a panel of 1687 diverse inbred lines from the USDA maize gene bank with 200,978 SNPs while controlling for background genetic relationships with a mixed model and identified seven single nucleotide polymorphisms (SNPs) in six genes associated with disease resistance in either the complete inbred panel (1687 lines with highly unbalanced phenotype data) or in a filtered inbred panel (734 lines with balanced phenotype data). Different sets of SNPs were detected as associated in the two different data sets. The alleles conferring greater disease resistance at all seven SNPs were rare overall (below 16%) and always higher in allele frequency in tropical maize than in temperate dent maize. Resampling analysis of the complete data set identified one robust SNP association detected as significant at a stringent rho-value in 94% of data sets, each representing a random sample of 80% of the lines. All associated SNPs were in exons, but none of the genes had predicted functions with an obvious relationship to resistance to fungal infection. Conclusions: GWAS in a very diverse maize collection identified seven SNP variants each associated with between 1% and 3% of trait variation. Because of their small effects, the value of selection on these SNPs for improving resistance to Fusarium ear rot is limited. Selection to combine these resistance alleles combined with genomic selection to improve the polygenic background resistance might be fruitful. The genes associated with resistance provide candidate gene targets for further study of the biological pathways involved in this complex disease resistance. C1 [Zila, Charles T.; Ogut, Funda] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Romay, Maria C.] Cornell Univ, Inst Genom Divers, Ithaca, NY 14853 USA. [Gardner, Candice A.] ARS, USDA, North Cent Reg Plant Intro Stn, Ames, IA 50014 USA. [Buckler, Edward S.] Cornell Univ, ARS, USDA, Plant Soil & Nutr Res Unit, Ithaca, NY 14853 USA. [Buckler, Edward S.] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. [Holland, James B.] N Carolina State Univ, ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. RP Holland, JB (reprint author), N Carolina State Univ, ARS, USDA, Plant Sci Res Unit, Raleigh, NC 27695 USA. EM james_holland@ncsu.edu RI Romay, Maria/B-8831-2015; OI Romay, Maria/0000-0001-9309-1586; Buckler, Edward/0000-0002-3100-371X; Holland, James/0000-0002-4341-9675 FU National Science Foundation [DBI-0321467, IOS-0820619, IOS-0604923]; USDA-ARS FX This research was supported by the National Science Foundation (projects DBI-0321467, IOS-0820619, and IOS-0604923) and by USDA-ARS. USDA-ARS supported the graduate fellowship of C.T. Zila. The authors wish to thank Jason Brewer, Steve Pigozzo, David Horne, Brittany Scott, Sarah Davidson-Dyer, and Colt Jackson for technical assistance, Bode Olukolu and Shang Xue for help with the analyses, Josie Bloom for help with laboratory procedures, Major Goodman and Matt Krakowsky for seed availability, and Sharon Mitchell, Charlotte Acharya, and Dallas Kroon of Cornell University for GBS data on new lines. NR 51 TC 11 Z9 11 U1 15 U2 52 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2229 J9 BMC PLANT BIOL JI BMC Plant Biol. PD DEC 30 PY 2014 VL 14 AR 372 DI 10.1186/s12870-014-0372-6 PG 15 WC Plant Sciences SC Plant Sciences GA CA0IW UT WOS:000348602400001 PM 25547028 ER PT J AU Piesik, D Delaney, KJ Bocianowski, J Ligor, M Buszewski, B AF Piesik, Dariusz Delaney, Kevin J. Bocianowski, Jan Ligor, Magdalena Buszewski, Boguslaw TI Silene latifolia temporal patterns of volatile induction and suppression after floral interaction by the nursery pollinator, Hadena bicruris (Lepidoptera: Noctuidae) SO ENTOMOLOGICA FENNICA LA English DT Article ID PLATANTHERA-BIFOLIA ORCHIDACEAE; NEIGHBORING UNINFECTED PLANTS; BEETLE XANTHOGALERUCA-LUTEOLA; POSTPOLLINATION CHANGES; LEAF BEETLE; BEHAVIORAL-RESPONSES; INSECT OVIPOSITION; PINUS-SYLVESTRIS; EGG PARASITOIDS; SCENT EMISSION AB After 24-hour Hadena bicruris floral interaction on Silene latifolia (Caryophyllaceae) with or without oviposition, we examined temporal volatile emission patterns for 3 days from plants with moth interaction and from neighboring plants only exposed to plant volatiles. Several lilac aldehydes and veratrole were progressively reduced after moth exposure without oviposition (by 30 to 40% after 24 hours and by 60 to 90% after 72 hours), but beta-myrcene and beta-pinene emissions increased by 200 to 300% only when exposure involved oviposition. Exposing S. latifolia to H. bicruris floral interaction without oviposition yielded no change in volatile organic compound (VOC) emission of neighboring S. latifolia; with oviposition, neighboring plants had 80 to 126% increases in emission rates for beta-myrcene and beta-pinene. Progressive reduction of S. latifolia VOC emission rates might help plants to avoid nursery pollinator oviposition. In contrast, with H. bicruris oviposition on S. latifolia flowers some VOCs (common herbivore-induced plant volatiles i.e. HIPVs) were induced. Whether oviposition occurred on S. latifolia strongly influenced neighboring plant VOC emission. C1 [Piesik, Dariusz] Univ Technol & Life Sci, Dept Entomol & Mol Phytopathol, PL-85225 Bydgoszcz, Poland. [Delaney, Kevin J.] ARS, Pest Management Res Unit, Northern Plains Agr Res Lab, USDA, Sidney, MT 59270 USA. [Bocianowski, Jan] Poznan Univ Life Sci, Dept Math & Stat Methods, PL-60637 Poznan, Poland. [Ligor, Magdalena; Buszewski, Boguslaw] Nicholas Copernicus Univ, Fac Chem, Chair Environm Chem & Bioanalyt, PL-87100 Torun, Poland. RP Piesik, D (reprint author), Univ Technol & Life Sci, Dept Entomol & Mol Phytopathol, 20 Kordeckiego St, PL-85225 Bydgoszcz, Poland. EM piesik@utp.edu.pl RI Buszewski, Boguslaw/A-3187-2009 FU Marie-Curie Foundation (FLORDETERSIGNALS) [MERG-CT-2007-200265]; European Re-integration Grant (ERG) entitled, "Silene floral and deterrent signals" FX We wish to thank R. B. Srygley (USDA-ARS) and two anonymous reviewers for providing feedback on earlier drafts that helped improve the quality of this manuscript. This research was supported with funds provided by grants from the Marie-Curie Foundation (FLORDETERSIGNALS, contract number MERG-CT-2007-200265), and European Re-integration Grant (ERG) entitled, "Silene floral and deterrent signals". The experiments were done at the laboratory of the Regional Center for Innovation (RCI) - University of Technology and Life Sciences in Bydgoszcz, Poland. NR 62 TC 1 Z9 2 U1 3 U2 9 PU ENTOMOLOGICA FENNICA PI HELSINKI PA C/O ZOOLOGICAL MUSEUM P RAUTATIEKATU 13 UNIV HELSINKI, FIN-00014 HELSINKI, FINLAND SN 0785-8760 J9 ENTOMOL FENNICA JI Entomol. Fenn. PD DEC 30 PY 2014 VL 25 IS 4 BP 199 EP 219 PG 21 WC Entomology SC Entomology GA AZ5EL UT WOS:000348243200005 ER PT J AU Trejo-Torres, JC Caraballo-Ortiz, MA Vives-Heyliger, MA Torres-Santana, CW Cetzal-Ix, W Mercado-Diaz, JA Carlo, TA AF Trejo-Torres, Jorge C. Caraballo-Ortiz, Marcos A. Vives-Heyliger, Miguel A. Torres-Santana, Christian W. Cetzal-Ix, William Mercado-Diaz, Joel A. Carlo, Tomas A. TI Rediscovery of Eugenia fajardensis (Myrtaceae), a rare tree from the Puerto Rican Bank SO PHYTOTAXA LA English DT Article DE Antilles; Caribbean; Culebra; Fajardo's big guava; "guayabota de Fajardo"; Puerto Rico; Vieques; West Indies AB Eugenia fragrans var.? fajardensis was described in 1895 and raised to species status in 1923 as E. fajardensis. In 1925, it was relegated to the synonymy of Anamomis fragrans (Myrcianthes fragrans). Since 2001, we have re-discovered wild plants and herbarium specimens, including a previously unidentified isotype of E. fajardensis, supporting the validity of this species. Here we designate a lectotype and an epitype for E. fajardensis. In addition, we provide: 1) an extended description for the species including the previously unknown flowers and fruits, an illustration, and photographs of live plants, 2) a key for the 24 taxa of Eugenia reported for Puerto Rico and the Virgin Islands, and 3) descriptions of the three known populations. These populations collectively hold 182 plants in the islands of Puerto Rico, Culebra, and Vieques. Based on the IUCN Red List Criteria, E. fajardensis meets the requirements to be considered a Critically Endangered species. C1 [Trejo-Torres, Jorge C.] Inst Reg Conservat, Delray Beach, FL 33483 USA. [Caraballo-Ortiz, Marcos A.; Mercado-Diaz, Joel A.] Univ Puerto Rico, Herbario UPR, San Juan, PR 00926 USA. [Torres-Santana, Christian W.] USDA, Int Inst Trop Forestry, Forest Serv, Jardin Bot sur, Rio Piedras, PR 00926 USA. [Cetzal-Ix, William] CICY, AC, Merida 97310, Yucatan, Mexico. [Caraballo-Ortiz, Marcos A.; Carlo, Tomas A.] Penn State Univ, Dept Biol, University Pk, PA 16802 USA. [Torres-Santana, Christian W.] Fdn Luis Munoz Marin, San Juan, PR 00926 USA. RP Trejo-Torres, JC (reprint author), Inst Reg Conservat, 100 East Linton Blvd,Suite 302B, Delray Beach, FL 33483 USA. EM karsensis@yahoo.com.mx FU USDA Forest Service International the Institute of Tropical Forestry [00-CA-1112-0105-001]; Ciudadanos del Karso, Inc. FX We thank: C. Clubbe, A. Lugo, N. Snow, and an anonymous reviewer for providing comments and suggestions to improve the manuscript; J. Aukema, M. Barandiaran, G. Burgos, R. Colon, O. Diaz, J. Garcia, W. Hernandez, J. Martinez, M. Mercado, A. Morales, P. J. Rivera, R. Rivera, A. Roman, E. Santiago, T. Talevast, the Herbarium UPR, and the USFWS for providing valuable logistic support during field expeditions; the curators of B, BM, C, F, FTG, GOET, HUH, JBSD, K, L, MAPR, NY, P, SJ, UPR, UPRRP, US, WAG, and W for facilitating the access to collections; J. Velez (MAPR) and F. Areces (UPRRP) for preparing digital images of selected specimens; M. Quinones for providing assistance on GIS and climate data; O. Diaz, O. Monsegur, C. Pacheco and J. Sustache for providing information on localities and abundance data; and G. Alvarado and J. Perez from El Nuevo Dia newspaper, and the Sierra Club Puerto Rico Chapter for divulging on E. fajardensis to the general public. JCTT thanks the USDA Forest Service International the Institute of Tropical Forestry for supporting initial research under cooperative agreement 00-CA-1112-0105-001 with Ciudadanos del Karso, Inc. NR 42 TC 0 Z9 0 U1 1 U2 6 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD DEC 30 PY 2014 VL 191 IS 1 BP 154 EP 164 PG 11 WC Plant Sciences SC Plant Sciences GA AY4LK UT WOS:000347549400010 ER PT J AU Carrera-Chavez, JM Hernandez-Ceron, J Lopez-Carlos, MA Lozano-Dominguez, RR Molinar, F Echavartia-Chairez, FG Banuelos-Valenzuela, R Arechiga-Flores, CF AF Carrera-Chavez, J. M. Hernandez-Ceron, J. Lopez-Carlos, M. A. Lozano-Dominguez, R. R. Molinar, F. Echavartia-Chairez, F. G. Banuelos-Valenzuela, R. Arechiga-Flores, C. F. TI Superovulatory response and embryo development in ewes treated with two doses of bovine somatotropin SO ANIMAL REPRODUCTION SCIENCE LA English DT Article DE Embryo transfer; Ewe; Somatotropin; Insulin; IGF-1 ID GROWTH-FACTOR-I; FACTOR SYSTEM RESPONSES; LACTATING DAIRY-COWS; PREGNANCY RATES; ESTROUS SYNCHRONIZATION; FOLLICLE DEVELOPMENT; OVARIAN-FOLLICLES; DOWN-REGULATION; OOCYTE QUALITY; LUTEAL-PHASE AB This study evaluated whether the administration of 50 and 100mg bovine somatotropin (bST) at the start of synchronization and at the time of natural mating in ewes improves the ovulation rate, embryonic development and pregnancy rate of transferred embryos. Forty-eight donors were assigned to three treatments: the bST-100 treatment (n = 15) received 100mg bST at the start of synchronization and at natural mating, the bST-50 treatment (n = 15) received 50mg bST on the same schedule as the previous group, and the control (n = 18) did not receive any bST. Two embryos were transferred to each recipient (n = 121): 35 received embryos from bST-100; 50 received embryos from bST-50, and 36 received embryos from the control. The superovulatory rate, percentage of recovered structures, cleavage rate, percentage of transferable embryos, embryo quality and development and pregnancy rate were analyzed using the GENMOD procedure of SAS. The number of corpora lutea and the cell number were analyzed using the GLM procedure of SAS. The insulin and IGF-1 concentrations were analyzed with ANOVA for repeated measures. The bST application did not affect the superovulatory rate, number of corpora lutea and recovered structures (P > 0.05). The numbers of transferable embryos and embryos reaching the blastocyst were higher (P <= 0.01) in the bST-50 (96.4 +/- 3.6% and 69.0 +/- 7.8%) than the bST-100 (93.0 +/- 4.5% and 27.2 +/- 38.9%) and control (87.7 +/- 5.4% and 50.4 +/- 6.4%) groups. The insulin and IGF-1 concentrations were higher (P < 0.05) in the bST-treated groups, but the insulin concentration was higher (P < 0.05) in the bST-100 group than in the bST-50 group. The pregnancy rate was similar (P = 0.21) in ewes receiving embryos from the two treatments [bST-50, (70.0%); bST-100, (62.5%), and control, (56.6%)]. The administration of 50mg bST at the start of synchronization and at natural mating in superovulated ewes was concluded to enhance the proportion and development of transferable embryos. However, bST did not affect the pregnancy rate of transferred embryos. (C) 2014 Published by Elsevier B.V. C1 [Carrera-Chavez, J. M.; Lopez-Carlos, M. A.; Lozano-Dominguez, R. R.; Echavartia-Chairez, F. G.; Banuelos-Valenzuela, R.; Arechiga-Flores, C. F.] Univ Autenoma Zacatecas, Unidad Acad Med Vet & Zootecn, Zacatecas 98500, Mexico. [Hernandez-Ceron, J.] Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecn, Mexico City 04510, DF, Mexico. [Molinar, F.] USDA, El Paso, TX 79936 USA. [Molinar, F.] Univ Autenoma Ciudad Juarez, Dept Ciencias Vet, Chihuahua 32315, Mexico. RP Arechiga-Flores, CF (reprint author), Univ Autenoma Zacatecas, Unidad Acad Med Vet & Zootecn, Carretera Panamer Zacatecas Fresnillo Km 31-5, Zacatecas 98500, Mexico. EM jose.carrera@uacj.mx; arechiga@uaz.edu.mx OI Lopez Carlos, Marco A./0000-0002-8163-5264; Carrera Chavez, Jose Maria/0000-0002-6899-9485 FU Fundacion Produce Zacatecas A.C. (Zacatecas, Mexico) [32-2010-0013] FX This study was partly supported by the Fundacion Produce Zacatecas A.C. (Zacatecas, Mexico) (Project number: 32-2010-0013). The authors thank MSD Salud Animal Mexico for the donation of somatotropin (Boostin-S) and Dr. Clara Mejia Murcia from Laboratorio de Endocrinologia del Departamento de Reproduccion de la Facultad de Medicina Veterinaria y Zootecnia de la Universidad Nacional Autonoma de Mexico for her assistance in the determination of hormone levels. NR 48 TC 2 Z9 2 U1 2 U2 8 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-4320 EI 1873-2232 J9 ANIM REPROD SCI JI Anim. Reprod. Sci. PD DEC 30 PY 2014 VL 151 IS 3-4 BP 105 EP 111 DI 10.1016/j.anireprosci.2014.10.009 PG 7 WC Agriculture, Dairy & Animal Science; Reproductive Biology SC Agriculture; Reproductive Biology GA AY3PA UT WOS:000347494100004 PM 25459075 ER PT J AU Rabotyagov, SS Campbell, TD White, M Arnold, JG Atwood, J Norfleet, ML Kling, CL Gassman, PW Valcu, A Richardson, J Turner, RE Rabalais, NN AF Rabotyagov, Sergey S. Campbell, Todd D. White, Michael Arnold, Jeffrey G. Atwood, Jay Norfleet, M. Lee Kling, Catherine L. Gassman, Philip W. Valcu, Adriana Richardson, Jeffrey Turner, R. Eugene Rabalais, Nancy N. TI Cost-effective targeting of conservation investments to reduce the northern Gulf of Mexico hypoxic zone SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE eutrophication; hypoxic zone; Gulf of Mexico; agricultural conservation practices; evolutionary computation ID WATER-QUALITY; OPTIMIZATION; POLLUTION; SCALE; RIVER AB A seasonally occurring summer hypoxic (low oxygen) zone in the northern Gulf of Mexico is the second largest in the world. Reductions in nutrients from agricultural cropland in its watershed are needed to reduce the hypoxic zone size to the national policy goal of 5,000 km(2) (as a 5-y running average) set by the national Gulf of Mexico Task Force's Action Plan. We develop an integrated assessment model linking the water quality effects of cropland conservation investment decisions on the more than 550 agricultural subwatersheds that deliver nutrients into the Gulf with a hypoxic zone model. We use this integrated assessment model to identify the most cost-effective subwatersheds to target for cropland conservation investments. We consider targeting of the location (which subwatersheds to treat) and the extent of conservation investment to undertake (how much cropland within a subwatershed to treat). We use process models to simulate the dynamics of the effects of cropland conservation investments on nutrient delivery to the Gulf and use an evolutionary algorithm to solve the optimization problem. Model results suggest that by targeting cropland conservation investments to the most cost-effective location and extent of coverage, the Action Plan goal of 5,000 km(2) can be achieved at a cost of $2.7 billion annually. A large set of cost-hypoxia tradeoffs is developed, ranging from the baseline to the nontargeted adoption of the most aggressive cropland conservation investments in all subwatersheds (estimated to reduce the hypoxic zone to less than 3,000 km(2) at a cost of $5.6 billion annually). C1 [Rabotyagov, Sergey S.; Richardson, Jeffrey] Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Campbell, Todd D.; Kling, Catherine L.; Gassman, Philip W.; Valcu, Adriana] Iowa State Univ Sci & Technol, Ctr Agr & Rural Dev, Dept Econ, Ames, IA 50011 USA. [White, Michael; Arnold, Jeffrey G.] USDA ARS, Grassland Soil & Water Res Lab, Temple, TX 76502 USA. [Atwood, Jay; Norfleet, M. Lee] USDA, Soil Sci & Resource Assessment Div, Nat Resources Conservat Serv, Temple, TX 76502 USA. [Turner, R. Eugene] Louisiana State Univ, Dept Oceanog & Coastal Sci, Baton Rouge, LA 70803 USA. [Rabalais, Nancy N.] Louisiana Univ Marine Consortium, Chauvin, LA 70344 USA. RP Rabotyagov, SS (reprint author), Univ Washington, Sch Environm & Forest Sci, Seattle, WA 98195 USA. EM rabotyag@uw.edu OI Kling, Catherine L/0000-0002-4785-7154 FU National Science Foundation; Dynamics of Coupled Natural and Human Systems Program [DEB-1010259]; US Department of Agriculture-National Institute of Food and Agriculture [2011-68002-30190, 2011-68005-30411] FX This project was supported by the National Science Foundation, Dynamics of Coupled Natural and Human Systems Program, Award DEB-1010259, and by US Department of Agriculture-National Institute of Food and Agriculture Awards 2011-68002-30190 and 2011-68005-30411. NR 32 TC 9 Z9 9 U1 4 U2 47 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD DEC 30 PY 2014 VL 111 IS 52 BP 18530 EP 18535 DI 10.1073/pnas.1405837111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AY2UL UT WOS:000347444400041 PM 25512489 ER PT J AU Lewis, JD Wilton, M Mott, GA Lu, WW Hassan, JA Guttman, DS Desveaux, D AF Lewis, Jennifer D. Wilton, Mike Mott, G. Adam Lu, Wenwan Hassan, Jana A. Guttman, David S. Desveaux, Darrell TI Immunomodulation by the Pseudomonas syringae HopZ Type III Effector Family in Arabidopsis SO PLOS ONE LA English DT Article ID DISEASE RESISTANCE GENE; BACTERIAL FLAGELLIN; YOPJ SUPERFAMILY; TOMATO DC3000; IMMUNITY; ACETYLATION; PERCEPTION; ACTIVATION; THALIANA; PATTERN AB Pseudomonas syringae employs a type III secretion system to inject 20-30 different type III effector (T3SE) proteins into plant host cells. A major role of T3SEs is to suppress plant immune responses and promote bacterial infection. The YopJ/HopZ acetyltransferases are a superfamily of T3SEs found in both plant and animal pathogenic bacteria. In P. syringae, this superfamily includes the evolutionarily diverse HopZ1, HopZ2 and HopZ3 alleles. To investigate the roles of the HopZ family in immunomodulation, we generated dexamethasone-inducible T3SE transgenic lines of Arabidopsis for HopZ family members and characterized them for immune suppression phenotypes. We show that all of the HopZ family members can actively suppress various facets of Arabidopsis immunity in a catalytic residuedependent manner. HopZ family members can differentially suppress the activation of mitogen-activated protein (MAP) kinase cascades or the production of reactive oxygen species, whereas all members can promote the growth of non-virulent P. syringae. Localization studies show that four of the HopZ family members containing predicted myristoylation sites are localized to the vicinity of the plasma membrane while HopZ3 which lacks the myristoylation site is at least partially nuclear localized, suggesting diversification of immunosuppressive mechanisms. Overall, we demonstrate that despite significant evolutionary diversification, all HopZ family members can suppress immunity in Arabidopsis. C1 [Lewis, Jennifer D.; Wilton, Mike; Mott, G. Adam; Lu, Wenwan; Guttman, David S.; Desveaux, Darrell] Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada. [Lewis, Jennifer D.] USDA, Ctr Plant Gene Express, Albany, CA USA. [Lewis, Jennifer D.; Hassan, Jana A.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA. [Guttman, David S.; Desveaux, Darrell] Univ Toronto, Ctr Anal Genome Evolut & Funct, Toronto, ON, Canada. RP Lewis, JD (reprint author), Univ Toronto, Dept Cell & Syst Biol, Toronto, ON, Canada. EM jdlewis@berkeley.edu FU Natural Sciences and Engineering Research Council of Canada; Canada Research Chair in Plant-Microbe Systems Biology or Comparative Genomics; Centre for the Analysis of Genome Evolution and Function; United States Department of Agriculture Agricultural Research Service [5335-21000- 040-00D] FX This work was supported by Natural Sciences and Engineering Research Council of Canada awards to DSG and DD; a Canada Research Chair in Plant-Microbe Systems Biology (DD) or Comparative Genomics (DSG); the Centre for the Analysis of Genome Evolution and Function (DD and DSG); United States Department of Agriculture Agricultural Research Service 5335-21000- 040-00D (JDL). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 7 Z9 7 U1 5 U2 15 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 29 PY 2014 VL 9 IS 12 DI 10.1371/journal.pone.0116152 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX7UN UT WOS:000347120200102 ER PT J AU Ghimire, B Williams, CA Masek, J Gao, F Wang, ZS Schaaf, C He, T AF Ghimire, Bardan Williams, Christopher A. Masek, Jeffrey Gao, Feng Wang, Zhuosen Schaaf, Crystal He, Tao TI Global albedo change and radiative cooling from anthropogenic land cover change, 1700 to 2005 based on MODIS, land use harmonization, radiative kernels, and reanalysis SO GEOPHYSICAL RESEARCH LETTERS LA English DT Article DE albedo; land cover change; radiative forcing; MODIS; global climate system ID CLIMATE FEEDBACKS; SECONDARY LANDS; USE TRANSITIONS; SURFACE ALBEDO; WOOD-HARVEST; SCALE; CIRCULATION; PRODUCTS; MODELS; IMPACT AB Widespread anthropogenic land cover change over the last five centuries has influenced the global climate system through both biogeochemical and biophysical processes. Models indicate that warming from carbon emissions associated with land cover conversion has been partially offset by cooling from elevated albedo, but considerable uncertainty remains partly because of uncertainty in model treatments of albedo. This study incorporates a new spatially and temporally explicit, land cover specific albedo product derived from Moderate Resolution Imaging Spectroradiometer with a historical land use data set (Land Use Harmonization product) to provide more precise, observationally derived estimates of albedo impacts from anthropogenic land cover change with a complete range of data set specific uncertainty. The mean annual global albedo increase due to land cover change during 1700-2005 was estimated as 0.001060.00008 (meanstandard deviation), mainly driven by snow exposure due to land cover transitions from natural vegetation to agriculture. This translates to a top-of-atmosphere radiative cooling of -0.150.1Wm(-2) (meanstandard deviation). Our estimate was in the middle of the Intergovernmental Panel on Climate Change Fifth Assessment Report range of -0.05 to -0.25Wm(-2) and incorporates variability in albedo within land cover classes. C1 [Ghimire, Bardan; Williams, Christopher A.] Clark Univ, Grad Sch Geog, Worcester, MA 01610 USA. [Ghimire, Bardan] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA. [Masek, Jeffrey; Wang, Zhuosen] NASA, Goddard Space Flight Ctr, Greenbelt, MD 20771 USA. [Gao, Feng] ARS, USDA, Beltsville, MD USA. [Wang, Zhuosen; Schaaf, Crystal] Univ Massachusetts, Sch Environm, Boston, MA 02125 USA. [He, Tao] Univ Maryland, Dept Geog Sci, College Pk, MD 20742 USA. RP Ghimire, B (reprint author), Clark Univ, Grad Sch Geog, Worcester, MA 01610 USA. EM bghimire@lbl.gov RI Masek, Jeffrey/D-7673-2012; He, Tao/H-5130-2012 OI He, Tao/0000-0003-2079-7988 FU NASA ROSES09 Science of Terra and Aqua Program [NNX11AG53G] FX This work was funded by a grant from the NASA ROSES09 Science of Terra and Aqua Program through award NNX11AG53G. The data used in this paper can be obtained by contacting the authors. NR 38 TC 3 Z9 3 U1 10 U2 30 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0094-8276 EI 1944-8007 J9 GEOPHYS RES LETT JI Geophys. Res. Lett. PD DEC 28 PY 2014 VL 41 IS 24 BP 9087 EP 9096 DI 10.1002/2014GL061671 PG 10 WC Geosciences, Multidisciplinary SC Geology GA CA4ZN UT WOS:000348916500053 ER PT J AU dos Santos, IC de Almeida, AAF Anhert, D da Conceicao, AS Pirovani, CP Pires, JL Valle, RR Baligar, VC AF dos Santos, Ivanildes C. de Almeida, Alex-Alan Furtado Anhert, Dario da Conceicao, Alessandro S. Pirovani, Carlos P. Pires, Jose L. Valle, Raul Rene Baligar, Virupax C. TI Molecular, Physiological and Biochemical Responses of Theobroma cacao L. Genotypes to Soil Water Deficit SO PLOS ONE LA English DT Article ID 2 CONTRASTING POPULATIONS; DROUGHT-STRESS; OXIDATIVE STRESS; ENVIRONMENTAL-STRESSES; SIGNAL-TRANSDUCTION; ADAPTIVE RESPONSES; GROWTH-RESPONSES; GENE-EXPRESSION; LEAF-AREA; PLANTS AB Six months-old seminal plants of 36 cacao genotypes grown under greenhouse conditions were subjected to two soil water regimes (control and drought) to assess, the effects of water deficit on growth, chemical composition and oxidative stress. In the control, soil moisture was maintained near field capacity with leaf water potentials (Psi WL) ranging from -0.1 to -0.5 MPa. In the drought treatment, the soil moisture was reduced gradually by withholding additional water until Psi WL reached values of between -2.0 to -2.5 MPa. The tolerant genotypes PS-1319, MO-20 and MA-15 recorded significant increases in guaiacol peroxidase activity reflecting a more efficient antioxidant metabolism. In relation to drought tolerance, the most important variables in the distinguishing contrasting groups were: total leaf area per plant; leaf, stem and total dry biomass; relative growth rate; plant shoot biomass and leaf content of N, Ca, and Mg. From the results of these analyses, six genotypes were selected with contrasting characteristics for tolerance to soil water deficit [CC-40, C. SUL-4 and SIC-2 (non-tolerant) and MA-15, MO-20, and PA-13 (tolerant)] for further assessment of the expression of genes NCED5, PP2C, psbA and psbO to water deficit. Increased expression of NCED5, PP2C, psbA and psbO genes were found for non-tolerant genotypes, while in the majority of tolerant genotypes there was repression of these genes, with the exception of PA-13 that showed an increased expression of psbA. Mutivariate analysis showed that growth variables, leaf and total dry biomass, relative growth rate as well as Mg content of the leaves were the most important factor in the classification of the genotypes as tolerant, moderately tolerant and sensitive to water deficit. Therefore these variables are reliable plant traits in the selection of plants tolerant to drought. C1 [dos Santos, Ivanildes C.; de Almeida, Alex-Alan Furtado; Anhert, Dario; da Conceicao, Alessandro S.; Pirovani, Carlos P.] Univ Estadual Santa Cruz, Dept Ciencias Biol, Ilheus, BA, Brazil. [Pires, Jose L.; Valle, Raul Rene] Comissao Execut Plano Lavoura Cacaueira CEPEC CEP, Ctr Pesquisas Cacau, BR-45650000 Ilheus, BA, Brazil. [Baligar, Virupax C.] ARS, USDA, Beltsville, MD 20705 USA. RP de Almeida, AAF (reprint author), Univ Estadual Santa Cruz, Dept Ciencias Biol, Campus Soane Nazare de Andrade,Rod Jorge Amado, Ilheus, BA, Brazil. EM alexalan@uesc.br NR 88 TC 0 Z9 0 U1 3 U2 25 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 26 PY 2014 VL 9 IS 12 AR e115746 DI 10.1371/journal.pone.0115746 PG 31 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX9RO UT WOS:000347239900076 ER PT J AU Beck, JJ Baig, N Cook, D Mahoney, NE Marsico, TD AF Beck, John J. Baig, Nausheena Cook, Daniel Mahoney, Noreen E. Marsico, Travis D. TI Semiochemicals from ex Situ Abiotically Stressed Cactus Tissue: A Contributing Role of Fungal Spores? SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE chalcogran; conophthorin; host plant volatiles; Opuntia humifusa; plant-insect-microbe interaction; spiroketal ID VOLATILE ORGANIC-COMPOUNDS; ELECTROPHYSIOLOGICAL RESPONSES; CACTOBLASTIS-CACTORUM; EMISSIONS; ALMONDS; CONOPHTHORIN; COLEOPTERA; PISTACHIO; BEETLES AB Semiochemicals play a central role in communication between plants and insects, such as signaling the location of a suitable host. Fungi on host plants can also play an influential role in communicating certain plant vulnerabilities to an insect. The spiroketal conophthorin is an important semiochemical produced by developing fungal spores. Spiroketals are also used as signals for scolytid communication. Plants and fungi are known to emit varying volatile profiles under biotic and abiotic stress. This paper reports distinctive temporal-volatile profiles from three abiotic treatments, room temperature (control), -15 degrees C (cold), and -15 degrees C to room temperature (shock), of cactus tissue plugs. Volatiles from the three treatments included monoterpenes from control plugs, compounds of varying classes and origin at later stages for cold plugs, and known semiochemicals, including spiroketals, at later stages for shock plugs. The results highlight several important findings: a unique tissue source of the spiroketals; abiotic cold-shock stress is indicated as the cause of spiroketal production; and, given previous findings of spirogenesis, fungal spore involvement is a probable biosynthetic origin of the spiroketals. These findings suggest an important role of fungal volatiles as signaling plant vulnerability to insects. C1 [Beck, John J.; Baig, Nausheena; Mahoney, Noreen E.] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA. [Cook, Daniel] ARS, Poisonous Plant Res Lab, USDA, Logan, UT 84341 USA. [Marsico, Travis D.] Arkansas State Univ, Dept Biol Sci, State Univ, AR 72467 USA. RP Beck, JJ (reprint author), ARS, Western Reg Res Ctr, USDA, 800 Buchanan St, Albany, CA 94710 USA. EM john.beck@ars.usda.gov FU USDA-ARS CRIS [5325-42000-037] FX Research was performed under USDA-ARS CRIS Project 5325-42000-037. NR 30 TC 4 Z9 4 U1 0 U2 12 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 24 PY 2014 VL 62 IS 51 BP 12273 EP 12276 DI 10.1021/jf505735g PG 4 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AX8CQ UT WOS:000347138500001 PM 25482874 ER PT J AU Luo, L Xu, ZL Yang, JY Xiao, ZL Li, YJ Beier, RC Sun, YM Lei, HT Wang, H Shen, YD AF Luo, Lin Xu, Zhen-Lin Yang, Jin-Yi Xiao, Zhi-Li Li, Yong-Jun Beier, Ross C. Sun, Yuan-Ming Lei, Hong-Tao Wang, Hong Shen, Yu-Dong TI Synthesis of Novel Haptens and Development of an Enzyme-Linked Immunosorbent Assay for Quantification of Histamine in Foods SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE histamine; haptens; ELISA ID BIOGENIC-AMINES; ORGANOPHOSPHORUS PESTICIDES; ANTIBODY; FISH; IMMUNOASSAY; SAMPLES; IDENTIFICATION; RECOGNITION; DERIVATIVES; PRODUCTS AB Novel haptens were designed and synthesized to prepare antibodies against free histamine, but none resulted in producing suitable antibodies for developing an enzyme-linked immunosorbent assay (ELISA). However, an antiserum was obtained having high specificity and affinity to p-nitrobenzoylated histamine (NPHA), which can be easily formed from reaction between histamine and p-nitrobenzoic acid N-hydroxysuccinimide ester (PNBA-OSu) under mild conditions. Based on rabbit polyclonal antibodies, a competitive indirect ELISA (ciELISA) for histamine determination in foods was developed. After ciELISA and derivatization optimization, the assay showed good sensitivity, with limits of detection of 1.8 mg/kg, 93.6 mu g/L, and 93.6 mu g/kg in fish, red wine, and yoghurt, respectively, with negligible cross-reactivity with related biogenic amines and amino acids. Average recovery of histamine in fortified food samples ranged from 80.9% to 110.1% with coefficients of variation below 16.3%. Good correlation between the ciELISA and liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. C1 [Luo, Lin; Xu, Zhen-Lin; Yang, Jin-Yi; Xiao, Zhi-Li; Sun, Yuan-Ming; Lei, Hong-Tao; Wang, Hong; Shen, Yu-Dong] South China Agr Univ, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China. [Li, Yong-Jun] Zhongshan Qual Supervis & Inspect Inst Agr Prod, Zhongshan 528403, Peoples R China. [Beier, Ross C.] ARS, Food & Feed Safety Res Unit, Southern Plains Agr Res Ctr, USDA, College Stn, TX 77845 USA. RP Sun, YM (reprint author), 483 Wushan Rd, Guangzhou 510642, Guangdong, Peoples R China. EM gzsyming@163.com; shenyudong@scau.edu.cn FU National Basic Research Program of China (973 Program) [2012CB720803]; Excellent Young Teachers Program on Higher Education of Guangdong Province; Guangdong Provincial Project of Science and Technology [2013B040402006, 2012A020100002] FX This work was supported by the National Basic Research Program of China (973 Program, 2012CB720803), the Excellent Young Teachers Program on Higher Education of Guangdong Province (Zhen-Lin Xu), and the Guangdong Provincial Project of Science and Technology (2013B040402006 and 2012A020100002). NR 39 TC 8 Z9 9 U1 7 U2 45 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 24 PY 2014 VL 62 IS 51 BP 12299 EP 12308 DI 10.1021/jf504689x PG 10 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AX8CQ UT WOS:000347138500005 PM 25417820 ER PT J AU Melendez-Martinez, AJ Paulino, M Stinco, CM Mapelli-Brahm, P Wang, XD AF Melendez-Martinez, Antonio J. Paulino, Margot Stinco, Carla M. Mapelli-Brahm, Paula Wang, Xiang-Dong TI Study of the Time-Course of cis/trans (Z/E) Isomerization of Lycopene, Phytoene, and Phytofluene from Tomato SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE carotenoids; colorless carotenoids; tomato products; geometrical isomers; nutrition; theoretical energy; food technology ID DIFFERENT GEOMETRICAL-ISOMERS; IN-VITRO; THERMAL-ISOMERIZATION; ANTIOXIDANT ACTIVITY; TRANS LYCOPENE; CAROTENOIDS; (Z)-ISOMERS; METABOLITES; STABILITY; PROSTATE AB In this study we investigated the formation of isomers of lycopene, phytoene, and phytofluene from tomato and their theoretical energy. The results indicated that certain (Z)-isomers are favored thermodynamically and/or kinetically over their (all-E)-counterparts. The relative percentages of (5Z)-lycopene in either thermodynamic or kinetic equilibria were approximately 33%, and those of (all-E)-lycopene were only approximately 22%. Most strikingly (15Z)-phytoene was the major isomer (>90%) when the thermodynamic or the kinetic equilibria were reached. These observations can explain the high levels of lycopene (Z)-isomers found in humans and their rapid formations upon additions of oil to tomato products. In addition, the results can be useful to predict the isomeric forms of lycopene, phytoene, and phytofluene expected in foods as well as in plasma and tissues upon ingestion. In light of the data in the present study, the use of certain geometrical isomers of phytoene, phytofluene and lycopene on their own or as mixtures is recommended in future studies aimed at assessing their possible bioactivity. C1 [Melendez-Martinez, Antonio J.; Stinco, Carla M.; Mapelli-Brahm, Paula] Univ Seville, Dept Nutr & Food Sci, Food Colour & Qual Lab, Seville, Spain. [Melendez-Martinez, Antonio J.; Wang, Xiang-Dong] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Nutr & Canc Biol Lab, Boston, MA 02111 USA. [Paulino, Margot] Univ Republica, Fac Quim, DETEMA, LaBioFarMol, Montevideo, Uruguay. RP Melendez-Martinez, AJ (reprint author), Univ Seville, Dept Nutr & Food Sci, Food Colour & Qual Lab, C-P Garcia Gonzalez 2, Seville, Spain. EM ajmelendez@us.es RI Stinco, Carla/M-8621-2014; Mapelli-Brahm, Paula/S-7865-2016 OI Stinco, Carla/0000-0003-2743-9016; Mapelli-Brahm, Paula/0000-0003-1940-442X FU Spanish State Secretariat of Research, Development and Innovation (Ministry of Economy and Competitiveness, CAROTFUNCT project) [AGL2012-37610]; FEDER; Andalusian Coundil of Economy, Innovation, Science and Employment [CAROTINCO-P12-AGR-1287]; CYTED [112RT0445] FX AJ.M.-M. acknowledges funding from the Spanish State Secretariat of Research, Development and Innovation (Ministry of Economy and Competitiveness, CAROTFUNCT project ref AGL2012-37610, cofunded by FEDER) and the Andalusian Coundil of Economy, Innovation, Science and Employment (project ref CAROTINCO-P12-AGR-1287). AJ.M.-M., M.P., C.M.S., and P.M.-B. are members of the IBERCAROT network, funded by CYTED (ref 112RT0445). NR 42 TC 5 Z9 5 U1 5 U2 19 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 24 PY 2014 VL 62 IS 51 BP 12399 EP 12406 DI 10.1021/jf5041965 PG 8 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AX8CQ UT WOS:000347138500016 PM 25426993 ER PT J AU Lewis, DL Breck, SW Wilson, KR Webb, CT AF Lewis, D. L. Breck, S. W. Wilson, K. R. Webb, C. T. TI Modeling black bear population dynamics in a human-dominated stochastic environment SO ECOLOGICAL MODELLING LA English DT Article DE Black bear; Elasticity; Sensitivity; Stochastic population growth; Ursus americanus ID URSUS-AMERICANUS; NORTH-AMERICA; NATIONAL-PARK; LIFE-HISTORY; MANAGEMENT; SURVIVAL; CARNIVORES; MOUNTAINS; REPRODUCTION; STRATEGIES AB Many large carnivore populations exist in human-influenced stochastic environments where availability of natural food sources vary annually and anthropogenic food sources can supplement energetic demands, but at a potential demographic cost due to human wildlife conflict and subsequent conflict management. Understanding how these competing factors influence a population is complex and difficult to study, but here we demonstrate the utility of using a stochastic projection matrix model and perturbation analysis to gain insight into this problem. We modeled a black bear population subjected to stochastic failures of fruiting and masting species, but with access to garbage in urban environments. We parameratized our model with data from a 6-year study on black bears in Aspen, Colorado and data synthesized from other research studies. Using computer simulation, we investigated the effect that different levels of conflict-bear removal can have on a bear population by comparing a "reference" scenario where bears did not benefit from human food sources or experience conflict-bear removals with two urban scenarios where bears had varying access to human foods, but conflict bears were removed. We used perturbation analyses to evaluate consequences for changing population vital rates and to estimate the impact each vital rate change had on population growth. Simulations were used to identify how much variation in each vital rate influenced variation in the population growth rate. We identified the survival rate of breeding adult females during good natural food years as having the highest elasticity value. We found that the benefit of increased cub production from available human food sources during natural food failure years was quickly negated if management of conflict bears through removal reduced adult female survival. Increasing the frequency of years when natural food production fails resulted in disproportionate impacts from available urban food and conflict-bear removals, where population growth rates in a High Removal scenario declined 1.5 times faster than in the reference scenario. Our findings suggest that for regions where changing climates will increase the frequency of natural food failures, managers may need to utilize non-lethal practices in managing conflict bears and municipalities will need to secure human food sources to reduce the need for conflict-bear removals and potential population declines. (C) 2014 Elsevier B.V. All rights reserved. C1 [Lewis, D. L.; Wilson, K. R.] Colorado State Univ, Dept Fish Wildlife & Conservat Biol, Ft Collins, CO 80523 USA. [Breck, S. W.] USDA WS Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA. [Webb, C. T.] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA. RP Lewis, DL (reprint author), Colorado State Univ, Dept Fish Wildlife & Conservat Biol, 1474 Campus Delivery, Ft Collins, CO 80523 USA. EM dsiwellewisd@gmail.com FU Colorado Parks and Wildlife (CPW); National Wildlife Research Center (NWRC); Berryman Institute FX We thank our funding sources Colorado Parks and Wildlife (CPW), National Wildlife Research Center (NWRC), and The Berryman Institute. We thank personnel from CPW, NWRC, City of Aspen and Pitkin County, Aspen Ranger district of the U.S. Forest Service, Aspen Field Biology Lab, Aspen Center for Environmental Studies, and field technicians. We also thank K. Crooks of Colorado State University for advice during the development of this manuscript. NR 54 TC 2 Z9 2 U1 12 U2 92 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-3800 EI 1872-7026 J9 ECOL MODEL JI Ecol. Model. PD DEC 24 PY 2014 VL 294 BP 51 EP 58 DI 10.1016/j.ecolmodel.2014.08.021 PG 8 WC Ecology SC Environmental Sciences & Ecology GA AU8BG UT WOS:000345821100006 ER PT J AU Bhalla, A Bischoff, KM Sani, RK AF Bhalla, Aditya Bischoff, Kenneth M. Sani, Rajesh K. TI Highly thermostable GH39 beta-xylosidase from a Geobacillus sp strain WSUCF1 SO BMC BIOTECHNOLOGY LA English DT Article DE Lignocellulose; Biofuels; beta-xylosidase; Thermostable ID SITE-DIRECTED MUTAGENESIS; GLYCOSIDE HYDROLASE; BIOCHEMICAL-CHARACTERIZATION; LIGNOCELLULOSE HYDROLYSIS; STEAROTHERMOPHILUS T-6; XYLO-OLIGOSACCHARIDES; XYLANASE; ENZYMES; BACILLUS; PURIFICATION AB Background: Complete enzymatic hydrolysis of xylan to xylose requires the action of endoxylanase and beta-xylosidase. beta-xylosidases play an important part in hydrolyzing xylo-oligosaccharides to xylose. Thermostable beta-xylosidases have been a focus of attention as industrially important enzymes due to their long shelf life and role in the relief of end-product inhibition of xylanases caused by xylo-oligosaccharides. Therefore, a highly thermostable beta-xylosidase with high specific activity has significant potential in lignocellulose bioconversion. Results: A gene encoding a highly thermostable GH39 beta-xylosidase was cloned from Geobacillus sp. strain WSUCF1 and expressed in Escherichia coli. Recombinant beta-xylosidase was active over a wide range of temperatures and pH with optimum temperature of 70 degrees C and pH 6.5. It exhibited very high thermostability, retaining 50% activity at 70 degrees C after 9 days. WSUCF1 beta-xylosidase is more thermostable than beta-xylosidases reported from other thermophiles (growth temperature <= 70 degrees C). Specific activity was 133 U/mg when incubated with p-nitrophenyl xylopyranoside, with K-m and V-max values of 2.38 mM and 147 U/mg, respectively. SDS-PAGE analysis indicated that the recombinant enzyme had a mass of 58 kDa, but omitting heating prior to electrophoresis increased the apparent mass to 230 kDa, suggesting the enzyme exists as a tetramer. Enzyme exhibited high tolerance to xylose, retained approximately 70% of relative activity at 210 mM xylose concentration. Thin layer chromatography showed that the enzyme had potential to convert xylo-oligomers (xylobiose, triose, tetraose, and pentaose) into fermentable xylose. WSUCF1 beta-xylosidase along with WSUCF1 endo-xylanase synergistically converted the xylan into fermentable xylose with more than 90% conversion. Conclusions: Properties of the WSUCF1 beta-xylosidase i.e. high tolerance to elevated temperatures, high specific activity, conversion of xylo-oligomers to xylose, and resistance to inhibition from xylose, make this enzyme potentially suitable for various biotechnological applications. C1 [Bhalla, Aditya; Sani, Rajesh K.] South Dakota Sch Mines & Technol, Dept Chem & Biol Engn, Rapid City, SD 57701 USA. [Bischoff, Kenneth M.] USDA ARS, Natl Ctr Agr Utilizat Res, Renewable Product Technol Res Unit, Peoria, IL 61604 USA. RP Sani, RK (reprint author), South Dakota Sch Mines & Technol, Dept Chem & Biol Engn, Rapid City, SD 57701 USA. EM Rajesh.Sani@sdsmt.edu FU National Science Foundation - Industry/University Cooperative Research Center (NSF-I/UCRC) [441087]; Department of Chemical and Biological Engineering at the South Dakota School of Mines and Technology FX The authors gratefully acknowledge the financial support provided by National Science Foundation - Industry/University Cooperative Research Center (NSF-I/UCRC, Grant #441087). The support from the Department of Chemical and Biological Engineering at the South Dakota School of Mines and Technology is gratefully acknowledged. NR 40 TC 6 Z9 7 U1 6 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1472-6750 J9 BMC BIOTECHNOL JI BMC Biotechnol. PD DEC 23 PY 2014 VL 14 AR 963 DI 10.1186/s12896-014-0106-8 PG 10 WC Biotechnology & Applied Microbiology SC Biotechnology & Applied Microbiology GA CC6WD UT WOS:000350508800002 PM 25532585 ER PT J AU Zhang, T Yu, LX McCord, P Miller, D Bhamidimarri, S Johnson, D Monteros, MJ Ho, J Reisen, P Samac, DA AF Zhang, Tiejun Yu, Long-Xi McCord, Per Miller, David Bhamidimarri, Suresh Johnson, David Monteros, Maria J. Ho, Julie Reisen, Peter Samac, Deborah A. TI Identification of Molecular Markers Associated with Verticillium Wilt Resistance in Alfalfa (Medicago Sativa L.) Using High-Resolution Melting SO PLOS ONE LA English DT Article ID ALBO-ATRUM; AUTOTETRAPLOID LUCERNE; VERTUS ALFALFA; LINKAGE MAPS; FORAGE YIELD; MODEL; MICROSATELLITE; MECHANISMS; TRUNCATULA; CULTIVARS AB Verticillium wilt, caused by the soilborne fungus, Verticillium alfalfae, is one of the most serious diseases of alfalfa (Medicago sativa L.) worldwide. To identify loci associated with resistance to Verticillium wilt, a bulk segregant analysis was conducted in susceptible or resistant pools constructed from 13 synthetic alfalfa populations, followed by association mapping in two F1 populations consisted of 352 individuals. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were used for genotyping. Phenotyping was done by manual inoculation of the pathogen to replicated cloned plants of each individual and disease severity was scored using a standard scale. Marker-trait association was analyzed by TASSEL. Seventeen SNP markers significantly associated with Verticillium wilt resistance were identified and they were located on chromosomes 1, 2, 4, 7 and 8. SNP markers identified on chromosomes 2, 4 and 7 co-locate with regions of Verticillium wilt resistance loci reported in M. truncatula. Additional markers identified on chromosomes 1 and 8 located the regions where no Verticillium resistance locus has been reported. This study highlights the value of SNP genotyping by high resolution melting to identify the disease resistance loci in tetraploid alfalfa. With further validation, the markers identified in this study could be used for improving resistance to Verticillium wilt in alfalfa breeding programs. C1 [Zhang, Tiejun; Yu, Long-Xi; McCord, Per] ARS, USDA, Prosser, WA 99350 USA. [Miller, David; Bhamidimarri, Suresh] DuPont Pioneer, Arlington, WI USA. [Johnson, David] Alforex Seeds, Salem, WI USA. [Monteros, Maria J.] Samuel Roberts Noble Fdn Inc, Forage Improvement Div, Ardmore, OK USA. [Ho, Julie; Reisen, Peter] Forage Genet Int Inc, Davis, CA USA. [Samac, Deborah A.] ARS, USDA, St Paul, MN USA. RP Yu, LX (reprint author), ARS, USDA, 24106 N Bunn Rd, Prosser, WA 99350 USA. EM Longxi.yu@ars.usda.gov FU USDA-ARS National Program [5354-21000-015-00D]; National Alfalfa and Forage Alliance FX This work was supported by USDA-ARS National Program Project No. 5354-21000-015-00D and by a grant from the National Alfalfa and Forage Alliance to L-X Yu. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 43 TC 6 Z9 6 U1 3 U2 30 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 23 PY 2014 VL 9 IS 12 AR e115953 DI 10.1371/journal.pone.0115953 PG 18 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AZ9UY UT WOS:000348563300092 PM 25536106 ER PT J AU Gagne, RJ Barosh, T Kephart, S AF Gagne, Raymond J. Barosh, Theresa Kephart, Susan TI A new species of Dasineura Rondani (Diptera: Cecidomyiidae) in flower galls of Camassia (Asparagaceae: Agavoideae) in the Pacific Northwest, USA SO ZOOTAXA LA English DT Article DE Agavoideae; Camassia; Cecidomyiidae; Dasineurini; flower gall midges; Lasiopteridi; Nearctic ID MIDGES DIPTERA; PHYLOGENY AB A new species, Dasineura camassiae Gagne (Diptera: Cecidomyiidae), is described, illustrated and compared to some of its congeners from related hosts and western North America. The new species causes flower galls on Camassia (Agavoideae; Asparagaceae) in the Pacific Northwest. Its current known distribution is Oregon and Washington, USA. Larvae develop in spring in flowers of Camassia spp., causing the young ovaries to enlarge prematurely and eventually abort, without forming seeds or mature fruit. Full-grown larvae crawl out of the gall in rapid succession and drop to the soil where they pupate; they remain there until spring of the following year when the adults emerge and lay eggs. The galls they induce in camas lily buds represent the first known association of the cosmopolitan genus Dasineura with the group of plants that includes Agave and its relatives. C1 [Gagne, Raymond J.] USDA ARS, Systemat Entomol Lab, PSI, Smithsonian Inst MRC 168, Washington, DC 20013 USA. [Barosh, Theresa] Colorado State Univ, Grad Degree Program Ecol, Ft Collins, CO 80523 USA. [Barosh, Theresa; Kephart, Susan] Willamette Univ, Dept Biol, Salem, OR 97301 USA. RP Gagne, RJ (reprint author), USDA ARS, Systemat Entomol Lab, PSI, Smithsonian Inst MRC 168, POB 37012, Washington, DC 20013 USA. EM raymond.gagne@ars.usda.gov; Theresa.Barosh@colostate.edu; skephart@willamette.edu FU M.J. Murdock Trust; NSF [DEB 1146531]; Botanical Society of America; Willamette University FX We are grateful for the help we received with this project from many individuals: Kathryn Theiss, Willamette University, for helpful contributions and logistical support in diverse aspects of this project, and for updating prior Liliaceae names for plant hosts of Cecidomyiidae; the many students and volunteers for field and laboratory assistance in collecting, surveying, and observing galls, in particular Natalie Amo, Emily Dick, Sean Fogerty, Kaliko Gadson, Linnea Hardlund, Florisela Herrejon-Chavez, Jim Kephart, Michael LaDouceur, Surabhi Mahajan, Rhys Ormond, and Jaime Peters; Peter Kolesik, Bionomics Ltd., Thebarton, SA, Australia, for ferreting out and sharing with us the de Stefani 1913 paper; Mike Althaus, Silver Spring, Maryland, for the final arrangement and labeling of Figs. 6-14; landowners John Marikos, the Lincolns, the Oregon Department of Transportation, The Nature Conservancy, and the U.S. Forest Service for invaluable access to field sites; and Christopher Borkent, California Department of Food and Agriculture, Sacramento, and Netta Dorchin, Tel Aviv University, Israel, for their close reading, helpful comments and insights on the final manuscript. This research was supported in part by: M.J. Murdock Trust and NSF DEB 1146531 grants (to SRK); Botanical Society of America and Willamette University Carson Awards (to TB). NR 18 TC 0 Z9 0 U1 0 U2 5 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1175-5326 EI 1175-5334 J9 ZOOTAXA JI Zootaxa PD DEC 22 PY 2014 VL 3900 IS 2 BP 271 EP 278 PG 8 WC Zoology SC Zoology GA AX4KW UT WOS:000346902500007 PM 25543738 ER PT J AU Hayashi, G Shen, Y Pedersen, TL Newman, JW Pook, M Cortopassi, G AF Hayashi, Genki Shen, Yan Pedersen, Theresa L. Newman, John W. Pook, Mark Cortopassi, Gino TI Frataxin deficiency increases cyclooxygenase 2 and prostaglandins in cell and animal models of Friedreich's ataxia SO HUMAN MOLECULAR GENETICS LA English DT Article ID AMYOTROPHIC-LATERAL-SCLEROSIS; MOUSE MODEL; OXIDATIVE STRESS; ALZHEIMERS-DISEASE; PHOSPHOLIPASE A(2); TRANSCRIPTION FACTORS; PARKINSONS-DISEASE; GENE-TRANSCRIPTION; ARACHIDONIC-ACID; EXPRESSION AB An inherited deficiency of the mitochondrial protein frataxin causes Friedreich's ataxia (FRDA); the mechanism by which this deficiency triggers neuro- and cardio-degeneration is unclear. Microarrays of neural tissue of animal models of the disease showed decreases in antioxidant genes, and increases in inflammatory genes. Cyclooxygenase (COX)-derived oxylipins are important mediators of inflammation. We measured oxylipin levels using tandem mass spectrometry and ELISAs in multiple cell and animal models of FRDA. Mass spectrometry revealed increases in concentrations of prostaglandins, thromboxane B2, 15-HETE and 11-HETE in cerebellar samples of knockin knockout mice. One possible explanation for the elevated oxylipins is that frataxin deficiency results in increased COX activity. While constitutive COX1 was unchanged, inducible COX2 expression was elevated over 1.35-fold (P<0.05) in two Friedreich's mouse models and Friedreich's lymphocytes. Consistent with higher COX2 expression, its activity was also increased by 58% over controls. COX2 expression is driven by multiple transcription factors, including activator protein 1 and cAMP response element-binding protein, both of which were elevated over 1.52-fold in cerebella. Taken together, the results support the hypothesis that reduced expression of frataxin leads to elevation of COX2-mediated oxylipin synthesis stimulated by increases in transcription factors that respond to increased reactive oxygen species. These findings support a neuroinflammatory mechanism in FRDA, which has both pathomechanistic and therapeutic implications. C1 [Hayashi, Genki; Shen, Yan; Cortopassi, Gino] Univ Calif Davis, Dept Mol Biosci, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, Dept Nutr, Davis, CA 95616 USA. [Pedersen, Theresa L.; Newman, John W.] ARS, USDA, Western Human Nutr Res Ctr, Davis, CA 95616 USA. [Newman, John W.] Univ Calif Davis, Genome Ctr, West Coast Metabol Ctr, Davis, CA 95616 USA. [Pook, Mark] Brunel Univ, Dept Biosci, Uxbridge UB8 3PH, Middx, England. RP Cortopassi, G (reprint author), Univ Calif Davis, Dept Mol Biosci, Davis, CA 95616 USA. EM gcortopassi@ucdavis.edu FU NIH [NS077777, EY012245, AG025532]; USDA-ARS [5306-51530-019-00D, 1 U24 DK097154-01] FX The study was supported by NIH grants NS077777, EY012245 and AG025532 to G.A.C., and USDA-ARS Intramural Projects 5306-51530-019-00D and 1 U24 DK097154-01 to J.W.N. Funding to pay the Open Access publication charges for this article was provided by the NIH. NR 52 TC 11 Z9 11 U1 0 U2 3 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 EI 1460-2083 J9 HUM MOL GENET JI Hum. Mol. Genet. PD DEC 20 PY 2014 VL 23 IS 25 BP 6838 EP 6847 DI 10.1093/hmg/ddu407 PG 10 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA AZ0GB UT WOS:000347923000013 PM 25104852 ER PT J AU Qi, QB Kilpelainen, TO Downer, MK Tanaka, T Smith, CE Sluijs, I Sonestedtl, E Chull, AY Renstrom, F Lin, XC Angquist, LH Huang, JY Liu, ZH Li, YP Ali, MA Xu, M Ahluwalia, TS Boer, JMA Chen, P Daimon, M Eriksson, J Perola, M Friedlander, Y Gao, YT Heppe, DHM Holloway, JW Houston, DK Kanoni, S Kim, YM Laaksonen, MA Jaaskelainen, T Lee, NR Lehtimaki, T Lemaitre, RN Lu, W Luben, RN Manichaiku, A Mannisto, S Marques-Vidal, P Monda, KL Ngwa, JS Perusse, L van Rooij, FJA Xiang, YB Wen, WQ Wojczynski, MK Zhu, JW Borecki, IB Bouchard, C Cai, QY Cooper, C Dedoussis, GV Deloukas, P Ferrucci, L Forouhi, NG Hansen, T Christiansen, L Hofman, A Johansson, I Jorgensen, T Karasawa, S Khaw, KT Kim, MK Kristiansson, K Li, HX Lin, X Liu, YM Lohman, KK Long, JR Mikkila, V Mozaffarian, D North, K Pedersen, O Raitakari, O Rissanen, H Tuomilehto, J van der Schouw, YT Uitterlinden, AG Zillikens, MC Franco, OH Tai, ES Shu, XO Siscovick, DS Toft, U Verschuren, WMM Vollenweider, P Wareham, NJ Witteman, JCM Zheng, W Ridker, PM Kang, JH Liang, LM Jensen, MK Curhan, GC Pasquale, LR Hunter, DJ Mohlke, KL Uusitupa, M Cupples, LA Rankinen, T Orho-Melander, M Wang, T Chasman, DI Franks, PW Sorensen, TIA Hu, FB Loos, RJF Nettleton, JA Qi, L AF Qi, Qibin Kilpelainen, Tuomas O. Downer, Mary K. Tanaka, Toshiko Smith, Caren E. Sluijs, Ivonne Sonestedtl, Emily Chull, Audrey Y. Renstrom, Frida Lin, Xiaochen Angquist, Lars H. Huang, Jinyan Liu, Zhonghua Li, Yanping Ali, Muhammad Asif Xu, Min Ahluwalia, Tarunveer Singh Boer, Jolanda M. A. Chen, Peng Daimon, Makoto Eriksson, Johan Perola, Markus Friedlander, Yechiel Gao, Yu-Tang Heppe, Denise H. M. Holloway, John W. Houston, Denise K. Kanoni, Stavroula Kim, Yu-Mi Laaksonen, Maarit A. Jaaskelainen, Tiina Lee, Nanette R. Lehtimaki, Terho Lemaitre, Rozenn N. Lu, Wei Luben, Robert N. Manichaiku, Ani Mannisto, Satu Marques-Vidal, Pedro Monda, Keri L. Ngwa, Julius S. Perusse, Louis van Rooij, Frank J. A. Xiang, Yong-Bing Wen, Wanqing Wojczynski, Mary K. Zhu, Jingwen Borecki, Ingrid B. Bouchard, Claude Cai, Qiuyin Cooper, Cyrus Dedoussis, George V. Deloukas, Panos Ferrucci, Luigi Forouhi, Nita G. Hansen, Torben Christiansen, Lene Hofman, Albert Johansson, Ingegerd Jorgensen, Torben Karasawa, Shigeru Khaw, Kay-Tee Kim, Mi-Kyung Kristiansson, Kati Li, Huaixing Lin, Xu Liu, Yongmei Lohman, Kurt K. Long, Jirong Mikkila, Vera Mozaffarian, Dariush North, Kari Pedersen, Oluf Raitakari, Olli Rissanen, Harri Tuomilehto, Jaakko van der Schouw, Yvonne T. Uitterlinden, Andre G. Zillikens, M. Carola Franco, Oscar H. Tai, E. Shyong Shu, Xiao Ou Siscovick, David S. Toft, Ulla Verschuren, W. M. Monique Vollenweider, Peter Wareham, Nicholas J. Witteman, Jacqueline C. M. Zheng, Wei Ridker, Paul M. Kang, Jae H. Liang, Liming Jensen, Majken K. Curhan, Gary C. Pasquale, Louis R. Hunter, David J. Mohlke, Karen L. Uusitupa, Matti Cupples, L. Adrienne Rankinen, Tuomo Orho-Melander, Marju Wang, Tao Chasman, Daniel I. Franks, Paul W. Sorensen, Thorkild I. A. Hu, Frank B. Loos, Ruth J. F. Nettleton, Jennifer A. Qi, Lu TI FTO genetic variants, dietary intake and body mass index: insights from 177 330 individuals SO HUMAN MOLECULAR GENETICS LA English DT Article ID OBESITY-ASSOCIATED GENE; TIME PHYSICAL-ACTIVITY; FAT MASS; ENERGY-INTAKE; FOOD-INTAKE; METABOLIC-RATE; ASSOCIATION; CHILDREN; RS9939609; GENOTYPE AB FTO is the strongest known genetic susceptibility locus for obesity. Experimental studies in animals suggest the potential roles of FTO in regulating food intake. The interactive relation among FTO variants, dietary intake and body mass index (BMI) is complex and results from previous often small-scale studies in humans are highly inconsistent. We performed large-scale analyses based on data from 177 330 adults (154 439 Whites, 5776 African Americans and 17 115 Asians) from 40 studies to examine: (i) the association between the FTO-rs9939609 variant (or a proxy single-nucleotide polymorphism) and total energy and macronutrient intake and (ii) the interaction between the FTO variant and dietary intake on BM I. The minor allele (A-allele) of the FTO-rs9939609 variant was associated with higher BMI in Whites (effect per allele = 0.34 [0.31, 0.37] kg/m(2), P = 1.9 x 10(-105)), and all participants (0.30 [0.30, 0.35] kg/m(2), P = 3.6 x 10(-107)). The BMI-increasing allele of the FTO variant showed a significant association with higher dietary protein intake (effect per allele = 0.08 [0.06, 0.10] %, P = 2.4 x 10(-16)), and relative weak associations with lower total energy intake (-6.4 [- 10.1, -2.6] kcal/day, P = 0.001) and lower dietary carbohydrate intake (-0.07 [- 0.11, -0.02] %, P = 0.004). The associations with protein (P = 7.5 x 10(-9)) and total energy (P = 0.002) were attenuated but remained significant after adjustment for BMI. We did not find significant interactions between the FTO variant and dietary intake of total energy, protein, carbohydrate or fat on BMI. Our findings suggest a positive association between the BMI-increasing allele of FTO variant and higher dietary protein intake and offer insight into potential link between FTO, dietary protein intake and adiposity. C1 [Qi, Qibin; Wang, Tao] Albert Einstein Coll Med, Dept Epidemiol, Bronx, NY 10467 USA. [Qi, Qibin; Downer, Mary K.; Li, Yanping; Ali, Muhammad Asif; Xu, Min; Mozaffarian, Dariush; Jensen, Majken K.; Hunter, David J.; Franks, Paul W.; Hu, Frank B.; Qi, Lu] Harvard Univ, Sch Publ Hlth, Dept Nutr, Boston, MA 02115 USA. [Lin, Xiaochen; Liu, Zhonghua; Mozaffarian, Dariush; Liang, Liming; Jensen, Majken K.; Curhan, Gary C.; Hunter, David J.; Franks, Paul W.; Hu, Frank B.] Harvard Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02115 USA. [Kilpelainen, Tuomas O.; Forouhi, Nita G.; Wareham, Nicholas J.; Loos, Ruth J. F.] Univ Cambridge, Sch Clin Med, Inst Metab Sci, MRC Epidemiol Unit, Cambridge, England. [Kilpelainen, Tuomas O.; Ahluwalia, Tarunveer Singh; Hansen, Torben; Pedersen, Oluf; Sorensen, Thorkild I. A.] Univ Copenhagen, Fac Hlth & Med Sci, Sect Metab Genet, Novo Nordisk Fdn Ctr Basic Metab Res, Copenhagen, Denmark. [Ahluwalia, Tarunveer Singh] Univ Copenhagen, Fac Hlth & Med Sci, Copenhagen Prospect Studies Asthma Childhood, Copenhagen, Denmark. [Tanaka, Toshiko; Ferrucci, Luigi] NIA, Translat Gerontol Branch, Baltimore, MD 21224 USA. [Smith, Caren E.] Tufts Univ, Jean Mayer USDA HNRCA, Nutr & Genom Lab, Boston, MA 02111 USA. [Sluijs, Ivonne; van der Schouw, Yvonne T.] Univ Med Ctr Utrecht, Julius Ctr Hlth Sci & Primary Care, Utrecht, Netherlands. [Sonestedtl, Emily; Renstrom, Frida; Orho-Melander, Marju; Franks, Paul W.] Lund Univ, Dept Clin Sci, Genet & Mol Epidemiol Unit, Malmo, Sweden. [Chull, Audrey Y.; Mozaffarian, Dariush; Kang, Jae H.; Curhan, Gary C.; Pasquale, Louis R.; Hunter, David J.; Hu, Frank B.; Qi, Lu] Brigham & Womens Hosp, Dept Med, Channing Div Network Med, Boston, MA 02115 USA. [Mozaffarian, Dariush; Ridker, Paul M.] Brigham & Womens Hosp, Div Cardiovasc Med, Boston, MA 02115 USA. [Chasman, Daniel I.] Brigham & Womens Hosp, Div Genet, Boston, MA 02115 USA. [Chasman, Daniel I.] Harvard Univ, Sch Med, Boston, MA USA. [Angquist, Lars H.; Sorensen, Thorkild I. A.] Bispebjerg & Frederiksberg Hosp, Inst Prevent Med, Copenhagen, Denmark. [Huang, Jinyan] Shanghai Jiao Tong Univ, Sch Med, Rui Jin Hosp, State Key Lab Med Genom,Shanghai Inst Hematol, Shanghai 200030, Peoples R China. [Ahluwalia, Tarunveer Singh] Gentofte Univ Hosp, Danish Pediat Asthma Ctr, Copenhagen, Denmark. [Boer, Jolanda M. A.; Verschuren, W. M. Monique] Natl Inst Publ Hlth & Environm, Ctr Nutr Prevent & Hlth Serv, NL-3720 BA Bilthoven, Netherlands. [Chen, Peng; Tai, E. Shyong] Natl Univ Hlth Syst, Natl Univ Singapore, Saw Swee Hock Sch Publ Hlth, Singapore, Singapore. [Tai, E. Shyong] Natl Univ Hlth Syst, Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Med, Singapore, Singapore. [Daimon, Makoto] Hirosaki Univ, Grad Sch Med, Dept Endocrinol & Metab, Hirosaki, Aomori, Japan. [Daimon, Makoto; Karasawa, Shigeru] Yamagata Univ, Fac Med, Dept Neurol Hematol Metab Endocrinol & Diabetol, Yamagata 990, Japan. [Eriksson, Johan] Univ Helsinki, Dept Gen Practice & Primary Hlth Care, Helsinki, Finland. [Perola, Markus] Univ Helsinki, Inst Mol Med, Helsinki, Finland. [Mikkila, Vera] Univ Helsinki, Dept Food & Environm Sci, Helsinki, Finland. [Perola, Markus; Laaksonen, Maarit A.; Mannisto, Satu; Kristiansson, Kati; Rissanen, Harri; Tuomilehto, Jaakko] Natl Inst Hlth & Welf, Helsinki, Finland. [Perola, Markus] Univ Tartu, Estonian Genome Ctr, EE-50090 Tartu, Estonia. [Friedlander, Yechiel] Hebrew Univ Jerusalem, Sch Publ Hlth, Jerusalem, Israel. [Gao, Yu-Tang; Xiang, Yong-Bing] Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Shanghai Canc Inst, Shanghai 200030, Peoples R China. [Heppe, Denise H. M.] Erasmus MC, Generat Study Grp R, Rotterdam, Netherlands. [Heppe, Denise H. M.; van Rooij, Frank J. A.; Hofman, Albert; Uitterlinden, Andre G.; Franco, Oscar H.; Witteman, Jacqueline C. M.] Erasmus MC, Dept Epidemiol, Rotterdam, Netherlands. [Heppe, Denise H. M.] Erasmus MC, Dept Pediat, Rotterdam, Netherlands. [Uitterlinden, Andre G.; Zillikens, M. Carola] Erasmus MC, Dept Internal Med, Rotterdam, Netherlands. [Holloway, John W.] Univ Southampton, Fac Med, Southampton SO9 5NH, Hants, England. [Houston, Denise K.] Wake Forest Sch Med, Dept Internal Med, Sect Gerontol & Geriatr Med, Winston Salem, NC USA. [Liu, Yongmei] Wake Forest Sch Med, Div Publ Hlth Sci, Dept Epidemiol, Winston Salem, NC USA. [Lohman, Kurt K.] Wake Forest Sch Med, Div Publ Hlth Sci, Dept Biostat Sci, Winston Salem, NC USA. [Kanoni, Stavroula; Deloukas, Panos] Queen Mary Univ London, Barts & London Sch Med & Dent, William Harvey Res Inst, London EC1M 6BQ, England. [Kim, Yu-Mi] Dong A Univ, Coll Med, Dept Prevent Med, Pusan, South Korea. [Jaaskelainen, Tiina; Uusitupa, Matti] Univ Eastern Finland, Inst Publ Hlth & Clin Nutr, Kuopio, Finland. [Lee, Nanette R.] Univ San Carlos, U5C Off Populat Studies Fdn Inc, Cebu, Philippines. [Lehtimaki, Terho] Univ Tampere, Dept Clin Chem, Fimlab Labs, FIN-33101 Tampere, Finland. [Lehtimaki, Terho] Univ Tampere, Sch Med, FIN-33101 Tampere, Finland. [Lemaitre, Rozenn N.; Siscovick, David S.] Univ Washington, Dept Med, Cardiovasc Hlth Res Unit, Seattle, WA USA. [Siscovick, David S.] Univ Washington, Dept Epidemiol, Cardiovasc Hlth Res Unit, Seattle, WA 98195 USA. [Lu, Wei] Shanghai Inst Prevent Med, Shanghai, Peoples R China. [Luben, Robert N.; Khaw, Kay-Tee] Univ Cambridge, Inst Publ Hlth, Dept Publ Hlth & Primary Care, Cambridge, England. [Manichaiku, Ani] Univ Virginia, Ctr Publ Hlth Genom, Charlottesville, VA USA. [Manichaiku, Ani] Univ Virginia, Dept Publ Hlth Sci, Div Biostat & Epidemiol, Charlottesville, VA USA. [Marques-Vidal, Pedro] Inst Social & Prevent Med, CH-1010 Lausanne, Switzerland. [Marques-Vidal, Pedro; Vollenweider, Peter] CHU Vaudois, Dept Med, CH-1011 Lausanne, Switzerland. [Monda, Keri L.; North, Kari] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [North, Kari] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC USA. [Mohlke, Karen L.] Univ N Carolina, Dept Genet, Chapel Hill, NC USA. [Monda, Keri L.] Amgen Inc, Ctr Observat Res, Thousand Oaks, CA 91320 USA. [Ngwa, Julius S.; Cupples, L. Adrienne] Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA USA. [Perusse, Louis] Univ Laval, Dept Kinesiol, Ste Foy, PQ, Canada. [van Rooij, Frank J. A.; Hofman, Albert; Uitterlinden, Andre G.; Zillikens, M. Carola; Franco, Oscar H.; Witteman, Jacqueline C. M.] NGI NCHA, Leiden, Netherlands. [Wen, Wanqing; Cai, Qiuyin; Long, Jirong; Shu, Xiao Ou; Zheng, Wei] Vanderbilt Univ, Sch Med, Vanderbilt Ingram Canc Ctr, Dept Med,Vanderbilt Epidemiol Ctr,Div Epidemiol, Nashville, TN 37212 USA. [Wojczynski, Mary K.; Borecki, Ingrid B.] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA. [Zhu, Jingwen; Li, Huaixing; Lin, Xu] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab, Shanghai, Peoples R China. [Zhu, Jingwen; Li, Huaixing; Lin, Xu] Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China. [Bouchard, Claude; Rankinen, Tuomo] Pennington Biomed Res Ctr, Human Genom Lab, Baton Rouge, LA 70808 USA. [Cooper, Cyrus] Univ Southampton, Southampton Gen Hosp, MRC Lifecourse Epidemiol Unit, Southampton SO16 6YD, Hants, England. [Cooper, Cyrus] Univ Southampton, Natl Inst Hlth Res Biomed Res Ctr, Southampton SO16 6YD, Hants, England. [Cooper, Cyrus] Univ Hosp Southampton NHS Fdn Trust, Southampton SO16 6YD, Hants, England. [Cooper, Cyrus] Univ Oxford, Natl Inst Hlth Res Musculoskeletal Biomed Res Uni, Oxford OX3 7LE, England. [Dedoussis, George V.] Harokopio Univ, Dept Dietet Nutr, Athens, Greece. [Deloukas, Panos] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England. [Deloukas, Panos] PACER HD, Jeddah 21589, Saudi Arabia. [Tuomilehto, Jaakko] King Abdulaziz Univ, Diabet Res Grp, Jeddah 21589, Saudi Arabia. [Christiansen, Lene] Univ Southern Denmark, Inst Publ Hlth, Danish Twin Registry, Odense, Denmark. [Johansson, Ingegerd] Umea Univ, Dept Odontol, Umea, Sweden. [Franks, Paul W.] Umea Univ, Dept Publ Hlth & Clin Med, Genet Epidemiol & Clin Res Grp, Umea, Sweden. [Jorgensen, Torben; Toft, Ulla] Glostrup Univ Hosp, Res Ctr Prevent & Hlth, Glostrup, Denmark. [Kim, Mi-Kyung] Hanyang Univ, Coll Med, Dept Prevent Med, Seoul 133791, South Korea. [Mikkila, Vera; Raitakari, Olli] Univ Turku, Res Ctr Appl & Prevent Cardiovasc Med, Turku, Finland. [Mozaffarian, Dariush] Tufts Univ, Friedman Sch Nutr Sci & Policy, Boston, MA 02111 USA. [Raitakari, Olli] Univ Turku, Dept Clin Physiol & Nucl Med, Turku, Finland. [Raitakari, Olli] Turku Univ Hosp, FIN-20520 Turku, Finland. [Tuomilehto, Jaakko] Danube Univ Krems, Ctr Vasc Prevent, A-3500 Krems, Austria. [Tuomilehto, Jaakko] Hosp Univ LaPaz IdiPAZ, Inst Invest Sanitaria, Madrid, Spain. [Tai, E. Shyong] Duke Natl Univ Singapore, Grad Sch Med, Singapore, Singapore. [Pasquale, Louis R.] Harvard Univ, Sch Med, Dept Ophthalmol, Mass Eye & Ear Infirm, Boston, MA USA. [Uusitupa, Matti] Kuopio Univ Hosp, Res Unit, SF-70210 Kuopio, Finland. [Cupples, L. Adrienne] Framingham Heart Dis Epidemiol Study, Framingham, MA USA. [Loos, Ruth J. F.] Mt Sinai Sch Med, Dept Prevent Med, Mindich Child Hlth & Dev Inst, Genet Obes & Related Metab Traits Program,Charles, New York, NY USA. [Nettleton, Jennifer A.] Univ Texas Hlth Sci Ctr Houston, Div Epidemiol Human Genet & Environm Sci, Houston, TX 77030 USA. RP Qi, L (reprint author), Harvard Univ, Sch Publ Hlth, Dept Nutr, 665 Huntington Ave, Boston, MA 02115 USA. EM nhlqi@channing.harvard.edu RI Holloway, John/B-5424-2009; Colaus, PsyColaus/K-6607-2013; Chen, Peng/E-5546-2015; Lin, Xiaochen/P-6460-2015; Sonestedt, Emily/I-3814-2016; Bouchard, Claude/A-7637-2009; Deloukas, Panos/B-2922-2013 OI Forouhi, Nita/0000-0002-5041-248X; Jorgensen, Torben/0000-0001-9453-2830; Tai, E Shyong/0000-0003-2929-8966; Franks, Paul/0000-0002-0520-7604; Holloway, John/0000-0001-9998-0464; Kristiansson, Kati/0000-0003-4688-107X; Mannisto, Satu/0000-0002-8668-3046; Luben, Robert/0000-0002-5088-6343; Chen, Peng/0000-0002-1422-4641; Sonestedt, Emily/0000-0002-0747-4562; Deloukas, Panos/0000-0001-9251-070X FU Canadian Institutes of Health Research [GR-15187, MOP-77652]; Cancer Research UK [, 14136]; FIC NIH HHS [TW008288, TW05596]; Medical Research Council [, G0401527, G0701863, G1000143, MC_U106179471, MC_UP_A100_1003, MC_UP_A620_1014, MC_UU_12011/1, MC_UU_12015/1, MC_UU_12015/5]; NCATS NIH HHS [UL1 TR000124, UL1TR000124]; NCI NIH HHS [CA047988, CA055075, CA49449, CA87969, P30 CA016086, R01CA064277, R01CA082729, R01CA092585, R01CA122364, R01CA124558, R01CA148667, R01CA82729, R37CA070867, U54CA155626, UM1 CA173640, UM1 CA182910]; NCRR NIH HHS [RR-024156, RR20649, UL1RR025005]; NEI NIH HHS [EY015473, R01 EY015473]; NHGRI NIH HHS [U01 HG004728, U01HG004399, U01HG004402, U01HG004728-02]; NHLBI NIH HHS [5R01 HL08770003, 5R01 HL08821502, HL-45670, HL043851, HL071981, HL073168, HL080295, HL080467, HL085144, HL085251, HL087652, HL088521, HL103612, HL105756, HL120393, HL34594, K08 HL112845, N01-HC-25195, N01-HC-95159, N01-HC-95169, N01HC55222, N01HC85079, N01HC85080, N01HC85081, N01HC85082, N01HC85083, N01HC85086, N02-HL-6-4278, N02‐HL‐6‐4278, R01 HL034594, R01 HL105756, R01 HL120393, R01HL071051, R01HL071205, R01HL071250, R01HL071251, R01HL071252, R01HL071258, R01HL071259, R01HL085710, R01HL086694, R01HL087641, R01HL59367]; NIA NIH HHS [AG023629, N01-AG-6-2101, N01-AG-6-2103, N01-AG-6-2106, R01-AG032098]; NIDDK NIH HHS [DK063491, 5P30DK46200, 5R01 DK06833603, 5R01 DK07568102, DK078150, DK080140, DK091718, DK46200, DK56350, DK58845, DK70756, P30 DK046200, R01 DK078150, R01 DK089256, R01 DK091718]; NIEHS NIH HHS [ES10126, P30 ES010126]; NIMHD NIH HHS [263 MD 821336, 263 MD 9164]; PHS HHS [HHSN268200625226C, HHSN268200782096C, HHSN268200800007C, HHSN268201100005C, HHSN268201100006C, HHSN268201100007C, HHSN268201100008C, HHSN268201100009C, HHSN268201100010C, HHSN268201100011C, HHSN268201100012C, HHSN268201200036C] NR 52 TC 24 Z9 24 U1 3 U2 23 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0964-6906 EI 1460-2083 J9 HUM MOL GENET JI Hum. Mol. Genet. PD DEC 20 PY 2014 VL 23 IS 25 BP 6961 EP 6972 DI 10.1093/hmg/ddu411 PG 12 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA AZ0GB UT WOS:000347923000023 PM 25104851 ER PT J AU Andorf, CM Kopylov, M Dobbs, D Koch, KE Stroupe, ME Lawrence, CJ Bass, HW AF Andorf, Carson M. Kopylov, Mykhailo Dobbs, Drena Koch, Karen E. Stroupe, M. Elizabeth Lawrence, Carolyn J. Bass, Hank W. TI G-Quadruplex (G4) Motifs in the Maize (Zea mays L.) Genome Are Enriched at Specific Locations in Thousands of Genes Coupled to Energy Status, Hypoxia, Low Sugar, and Nutrient Deprivation SO JOURNAL OF GENETICS AND GENOMICS LA English DT Article DE Maize; G-quadruplex; G4; Hypoxia; Sucrose synthase ID PROMOTER G-QUADRUPLEX; DNA G-QUADRUPLEXES; END RULE PATHWAY; ARABIDOPSIS-THALIANA; FORMING SEQUENCES; WIDE ANALYSIS; PROLYL HYDROXYLATION; NUCLEOTIDE-SEQUENCES; REGULATORY ELEMENTS; PROXIMAL PROMOTER AB The G-quadruplex (G4) elements comprise a class of nucleic acid structures formed by stacking of guanine base quartets in a quadruple helix. This G4 DNA can form within or across single-stranded DNA molecules and is mutually exclusive with duplex B-form DNA. The reversibility and structural diversity of G4s make them highly versatile genetic structures, as demonstrated by their roles in various functions including telomere metabolism, genome maintenance, immunoglobulin gene diversification, transcription, and translation. Sequence motifs capable of forming G4 DNA are typically located in telomere repeat DNA and other non-telomeric genomic loci. To investigate their potential roles in a large-genome model plant species, we computationally identified 149,988 non-telomeric G4 motifs in maize (Zea mays L., B73 AGPv2), 29% of which were in non-repetitive genomic regions. G4 motif hotspots exhibited non-random enrichment in genes at two locations on the antisense strand, one in the 50 UTR and the other at the 50 end of the first intron. Several genic G4 motifs were shown to adopt sequence-specific and potassium-dependent G4 DNA structures in vitro. The G4 motifs were prevalent in key regulatory genes associated with hypoxia (group VII ERFs), oxidative stress (DJ-1/GATase1), and energy status (AMPK/SnRK) pathways. They also showed statistical enrichment for genes in metabolic pathways that function in glycolysis, sugar degradation, inositol metabolism, and base excision repair. Collectively, the maize G4 motifs may represent conditional regulatory elements that can aid in energy status gene responses. Such a network of elements could provide a mechanistic basis for linking energy status signals to gene regulation in maize, a model genetic system and major world crop species for feed, food, and fuel. C1 [Andorf, Carson M.] Iowa State Univ, USDA ARS, Corn Insects & Crop Genet Res Unit, Ames, IA 50011 USA. [Andorf, Carson M.] Iowa State Univ, Dept Comp Sci, Ames, IA 50011 USA. [Kopylov, Mykhailo; Stroupe, M. Elizabeth] Florida State Univ, Inst Mol Biophys, Tallahassee, FL 32306 USA. [Dobbs, Drena; Lawrence, Carolyn J.] Iowa State Univ, Dept Genet Dev & Cell Biol, Ames, IA 50011 USA. [Koch, Karen E.] Univ Florida, Genet Inst, Dept Hort Sci, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA. [Stroupe, M. Elizabeth; Bass, Hank W.] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 USA. [Lawrence, Carolyn J.] Iowa State Univ, Dept Agron, Ames, IA 50011 USA. RP Bass, HW (reprint author), Florida State Univ, Dept Biol Sci, B-157, Tallahassee, FL 32306 USA. EM bass@bio.fsu.edu OI Bass, Hank/0000-0003-0522-0881 FU USDA-ARS; National Science Foundation [PGRP IOS-1025954, PGRP IOS-1116561]; U.S. Department of Agriculture (NRI-Plant Biochemistry) [07-03580]; Florida State University [0000025471] FX We are grateful to M. Schaeffer (at MaizeGDB.org) for assistance with maize gene nomenclature, to B.P. Chadwick, H. Cui, J.H. Dennis and G.G. Hoffman for helpful discussions, and to J. Kennedy for editorial assistance. This work was supported by USDA-ARS and grants from the National Science Foundation (PGRP IOS-1025954 to HWB, PGRP IOS-1116561 to KEK and coworkers), the U.S. Department of Agriculture (NRI-Plant Biochemistry 07-03580 to KEK and coworkers) and The Florida State University (CRC Planning Grant to HWB, OMNI award No. 0000025471). NR 133 TC 7 Z9 7 U1 2 U2 22 PU SCIENCE PRESS PI BEIJING PA 16 DONGHUANGCHENGGEN NORTH ST, BEIJING 100717, PEOPLES R CHINA SN 1673-8527 EI 1873-5533 J9 J GENET GENOMICS JI J. Genet. Genomics PD DEC 20 PY 2014 VL 41 IS 12 BP 627 EP 647 DI 10.1016/j.jgg.2014.10.004 PG 21 WC Biochemistry & Molecular Biology; Genetics & Heredity SC Biochemistry & Molecular Biology; Genetics & Heredity GA AW7CB UT WOS:000346421000002 PM 25527104 ER PT J AU Lucking, R Johnston, MK Aptroot, A Kraichak, E Lendemer, JC Boonpragob, K Caceres, MES Ertz, D Ferraro, LI Jia, ZF Kalb, K Mangold, A Manoch, L Mercado-Diaz, JA Moncada, B Mongkolsuk, P Papong, KB Parnmen, S Pelaez, RN Poengsungnoen, V Plata, ER Saipunkaew, W Sipman, HJM Sutjaritturakan, J Van den Broeck, D Von Konrat, M Weerakoon, G Lumbsch, HT AF Luecking, Robert Johnston, Mark K. Aptroot, Andre Kraichak, Ekaphan Lendemer, James C. Boonpragob, Kansri Caceres, Marcela E. S. Ertz, Damien Ferraro, Lidia Itati Jia, Ze-Feng Kalb, Klaus Mangold, Armin Manoch, Leka Mercado-Diaz, Joel A. Moncada, Bibiana Mongkolsuk, Pachara Papong, Khwanruan Butsatorn Parnmen, Sittiporn Pelaez, Rouchi N. Poengsungnoen, Vasun Plata, Eimy Rivas Saipunkaew, Wanaruk Sipman, Harrie J. M. Sutjaritturakan, Jutarat Van den Broeck, Dries Von Konrat, Matt Weerakoon, Gothamie Lumbsch, H. Thorsten TI One hundred and seventy-five new species of Graphidaceae: closing the gap or a drop in the bucket? SO PHYTOTAXA LA English DT Article DE Gomphillaceae; Graphis; Ocellularia; Thelotremataceae ID ASCOMYCOTA OSTROPALES GRAPHIDACEAE; LICHEN FAMILY THELOTREMATACEAE; TICOLICHEN BIODIVERSITY INVENTORY; TAKHTAJANS FLORISTIC REGIONS; CAPTURE PROBABILITIES VARY; GENUS GRAPHIS GRAPHIDACEAE; SOUTHEASTERN NORTH-AMERICA; WORLD-WIDE KEY; THELOTREMOID GRAPHIDACEAE; SOUTH-KOREA AB Recent studies of the global diversity of the lichenized fungal family Graphidaceae suggest that there are a large number of species remaining to be discovered. No less than 640 species have been described since 2002, including 175 new species introduced in a collaborative global effort in a single issue in this journal. These findings suggest that the largest family of tropical crustose lichens may have an even higher number of species than Parmeliaceae. To estimate whether the discovery of 175 new species is a significant step forward in cataloguing extant diversity in this family, we employed a parametric method to predict global species richness of Graphidaceae using a GIS-based grid map approach. The model employs linear regression between observed species richness and sample score and vegetation composition per grid to predict individual grid species richness, and interpolation of species grid distributions to predict global species richness. We also applied a non-parametric species-area curve approach and non-parametric species richness estimators (Chao, Jackknife, Bootstrap) to compare the results from the different methods. Our approach resulted in a prediction of 4,330 species of Graphidaceae, including approximately 3,500 (sub-) tropical species in the core subfamilies Fissurinoideae, Graphidoideae, Redonographoideae, plus 125 species restricted to extratropical regions (outside the zone between 30 degrees northern and 30 degrees southern latitude) and 700 species in subfamily Gomphilloideae. Currently, nearly 2,500 species are known in the family, including species not yet formally described. Thus, our model suggests that even after describing 175 species in this issue and with another approximately 140 awaiting publication, the number of species still to be discovered and described is more than 1,800, and much work remains to be done to close this substantial gap. Based on our approach, we predict that most of this undiscovered diversity is to be found in Mexico, the northern Andean region, the eastern Amazon and central and southern Brazil, tropical West Africa, continental Southeast Asia, Indonesia, and Papua New Guinea. C1 [Luecking, Robert; Johnston, Mark K.; Kraichak, Ekaphan; Lumbsch, H. Thorsten] ABL Herbarium, NL-3762 XK Soest, Netherlands. [Aptroot, Andre] New York Bot Garden, Inst Systemat Bot, Bronx, NY 10458 USA. [Lendemer, James C.] Ramkhamhang Univ, Fac Sci, Dept Biol, Lichen Res Unit, Bangkok 10240, Thailand. [Caceres, Marcela E. S.; Ertz, Damien] Univ Fed Sergipe, Dept Biociencias, BR-49500000 Itabaiana, Sergipe, Brazil. [Van den Broeck, Dries] Bot Garden Meise, Dept Bryophytes Thallophytes, B-1860 Meise, Belgium. [Ertz, Damien; Ferraro, Lidia Itati] Inst Botan Nordeste, RA-3400 Corrientes, Argentina. [Kalb, Klaus; Mangold, Armin] Liaocheng Univ, Coll Life Sci, Liaocheng 252059, Peoples R China. [Mangold, Armin; Manoch, Leka; Mercado-Diaz, Joel A.] Lichenolog Inst Neumarkt, D-92318 Neumarkt, Germany. [Mercado-Diaz, Joel A.; Moncada, Bibiana] Univ Regensburg, Inst Bot, D-93040 Regensburg, Germany. [Papong, Khwanruan Butsatorn; Parnmen, Sittiporn] Kasetsart Univ, Fac Agr, Dept Plant Pathol, Bangkok 10900, Thailand. [Poengsungnoen, Vasun; Plata, Eimy Rivas] USDA Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Plata, Eimy Rivas; Sipman, Harrie J. M.] Univ Distrital Francisco Jose Caldas, Bogota, Colombia. [Saipunkaew, Wanaruk; Sipman, Harrie J. M.] Mahsarakham Univ, Fac Sci, Dept Biol, Kantarawichai 44150, Maha Sarakham P, Thailand. [Van den Broeck, Dries; Von Konrat, Matt] Minist Publ Hlth, Dept Med Sci, Toxicol & Biochem Sect, Nonthaburi 11000, Thailand. [Papong, Khwanruan Butsatorn; Parnmen, Sittiporn] Kasetsart Univ, Fac Agr, Bangkok 10900, Thailand. [Plata, Eimy Rivas; Saipunkaew, Wanaruk] Chiang Mai Univ, Fac Sci, Dept Biol, Chiang Mai 50200, Thailand. [Weerakoon, Gothamie] Bot Garten & Bot Museum Berlin Dahlem, D-14195 Berlin, Germany. [Von Konrat, Matt] Univ Sri Jayawardenepura, Dept Bot, Jayawardenepura, Sri Lanka. RP Lucking, R (reprint author), Field Museum, 1400 South Lake Shore Dr, Chicago, IL 60605 USA. EM rlucking@fieldmuseum.org; mjohnston@fieldmuseum.org; andreaptroot@gmail.com; jlendemer@nybg.org; kansri@ru.ac.th; mscaceres@hotmail.com; damien.ertz@br.fgov.be; itati_liq@yahoo.com.ar; zfjia2008@163.com; klaus.kalb@arcor.de; arminmangold@gmail.com; agrlkm@ku.ac.th; joel_pr19@hotmail.com; bibianamoncada@yahoo.com; khwanruanpapong@gmail.com; sparnmen@gmail.com; rouchinadine@hotmail.com; vasun_poeng@hotmail.com; h.sipman@bgbm.org; dries.van.den.broeck@br.fgov.be; mvonkonrat@fieldmuseum.org; gothamiew@yahoo.com; tlumbsch@fieldmuseum.org RI Caceres, Marcela/H-7039-2012 FU National Science Foundation: TICOLICHEN [DEB 0206125]; Phylogeny and Taxonomy of Ostropalean Fungi; Emphasis on the Lichen-forming Thelotremataceae [DEB 0516116]; Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms [DEB 715660]; ATM-Assembling a taxonomic monograph: The lichen family Graphidaceae [DEB-1025861]; Caterpillar(R) company FX Data obtained for this study were gathered as part of several projects funded by the National Science Foundation: TICOLICHEN (DEB 0206125 to The Field Museum; PI Robert Lucking), Phylogeny and Taxonomy of Ostropalean Fungi, with Emphasis on the Lichen-forming Thelotremataceae (DEB 0516116 to The Field Museum; PI H. T. Lumbsch; Co-PI R. Lucking), Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms (DEB 715660 to The Field Museum; PI R. Lucking), and ATM-Assembling a taxonomic monograph: The lichen family Graphidaceae (DEB-1025861 to The Field Museum; PI T. Lumbsch, CoPI R. Lucking). The Caterpillar (R) company provided funds to study lichens and other cryptogams from Panama, especially with regard to molecular approaches. The curators and collections managers at the many herbaria from which type specimens and other collections were studied are thanked for their collaboration. Without their continuous efforts, a work such as the present one would not be possible. NR 269 TC 34 Z9 34 U1 1 U2 13 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD DEC 19 PY 2014 VL 189 IS 1 SI SI BP 7 EP 38 PG 32 WC Plant Sciences SC Plant Sciences GA AY4LH UT WOS:000347549100002 ER PT J AU Lumbsch, HT Kraichak, E Parnmen, S Plata, ER Aptroot, A Caceres, MES Ertz, D Feuerstein, SC Mercado-Diaz, JA Staiger, B Van den Broeck, D Lucking, R AF Lumbsch, H. Thorsten Kraichak, Ekaphan Parnmen, Sittiporn Plata, Eimy Rivas Aptroot, Andre Caceres, Marcela E. S. Ertz, Damien Feuerstein, Shirley Cunha Mercado-Diaz, Joel A. Staiger, Bettina Van den Broeck, Dries Luecking, Robert TI New higher taxa in the lichen family Graphidaceae (lichenized Ascomycota: Ostropales) based on a three-gene skeleton phylogeny SO PHYTOTAXA LA English DT Article DE Brazil; classification; Diploschistaceae; Puerto Rico; Xalocoa ID RIBOSOMAL DNA; LECANOROMYCETES OSTROPALES; MOLECULAR PHYLOGENY; GENUS TOPELIOPSIS; NORTH-AMERICA; THELOTREMATACEAE; ALIGNMENT; SUBUNIT; INFERENCE; CLADE AB We provide an updated skeleton phylogeny of the lichenized family Graphidaceae (excluding subfamily Gomphilloideae), based on three loci (mtSSU, nuLSU, RPB2), to elucidate the position of four new genera, Aggregatorygma, Borinquenotrema, Corticorygma, and Paratopeliopsis, as well as the placement of the enigmatic species Diorygma erythrellum, Fissurina monilifera, and Redingeria desseiniana. Based on the resulting topology, in addition to three tribes described previously, we recognize four further tribes in the subfamily Graphidoideae: Acanthothecieae Lumbsch, Kraichak & Lucking, Diploschisteae (Zahlbr.) Lumbsch, Kraichak & Lucking, Leptotremateae Lumbsch, Kraichak & Lucking, and Wirthiotremateae Lumbsch, Kraichak & Lucking. The phylogenetic position of Aggregatorygma and Borinquenotrema was not resolved with support, whereas Corticorygma forms part of Acanthothecieae, supported sister to Acanthothecis, and Paratopeliopsis belongs in Thelotremateae, unsupported sister to Leucodecton. Diorygma erythrellum is confirmed as a member of the Diorygma-Thalloloma clade, while Fissurina monilifera, inspite of its myriotremoid ascomata, belongs in Fissurina s.str. Redingeria dessiniana, although resembling the genus Phaeographopsis, is supported sister to R. glaucoglyphica. Topeliopsis darlingtonii forms the sister group to Gintarasia megalophthalma. Consequently, T. darlingtonii and the closely related T. elixii are recombined in Gintarasia as Gintarasia darlingtonii (Frisch & Kalb) Lumbsch, Kraichak & Lucking, and G. elixii (Frisch & Kalb) Lumbsch, Kraichak & Lucking. C1 [Lumbsch, H. Thorsten; Kraichak, Ekaphan; Plata, Eimy Rivas; Luecking, Robert] Field Museum, Chicago, IL 60605 USA. [Parnmen, Sittiporn] Minist Publ Hlth, Dept Med Sci, Toxicol & Biochem Sect, Nonthaburi 11000, Thailand. [Aptroot, Andre] ABL Herbarium, NL-3762 XK Soest, Netherlands. [Caceres, Marcela E. S.] Univ Fed Sergipe, Dept Biociencias, BR-49500000 Itabaiana, Sergipe, Brazil. [Ertz, Damien] Natl Bot Garden Belgium, Dept Bryophytes Thallophytes, B-1860 Meise, Belgium. [Feuerstein, Shirley Cunha] Univ Fed Parana, Dept Bot, Lab Liquenol, BR-81531970 Curitiba, PR, Brazil. [Mercado-Diaz, Joel A.] USDA Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Staiger, Bettina] Univ Regensburg, Inst Bot, D-93040 Regensburg, Germany. RP Lumbsch, HT (reprint author), Field Museum, 1400 South Lake Shore Dr, Chicago, IL 60605 USA. EM sparnmen@gmail.com; andreaptroot@gmail.com; mscaceres@hotmail.com; damien.ertz@br.fgov.be; shirleycunha_@hotmail.com; joel_pr19@hotmail.com; dries.van.den.broeck@br.fgov.be; rlucking@fieldmuseum.org RI Caceres, Marcela/H-7039-2012 FU National Science Foundation: TICOLICHEN [DEB 0206125]; Phylogeny and Taxonomy of Ostropalean Fungi; Emphasis on the Lichen-forming Thelotremataceae [DEB 0516116]; Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms [DEB 715660]; ATM-Assembling a taxonomic monograph: The lichen family Graphidaceae [DEB-1025861] FX Data obtained for this study were gathered as part of several projects funded by the National Science Foundation: TICOLICHEN (DEB 0206125 to The Field Museum; PI Robert Lucking), Phylogeny and Taxonomy of Ostropalean Fungi, with Emphasis on the Lichen-forming Thelotremataceae (DEB 0516116 to The Field Museum; PI H. T. Lumbsch; Co-PI R. Lucking), Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms (DEB 715660 to The Field Museum; PI R. Lucking), and ATM-Assembling a taxonomic monograph: The lichen family Graphidaceae (DEB-1025861 to The Field Museum; PI T. Lumbsch, CoPI R. Lucking). NR 61 TC 10 Z9 10 U1 0 U2 3 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD DEC 19 PY 2014 VL 189 IS 1 SI SI BP 39 EP 51 PG 13 WC Plant Sciences SC Plant Sciences GA AY4LH UT WOS:000347549100003 ER PT J AU Kraichak, E Parnmen, S Lucking, R Plata, ER Aptroot, A Caceres, MES Ertz, D Mangold, A Mercado-Diaz, JA Papong, K Van den Broeck, D Weerakoon, G Lumbsch, HT AF Kraichak, Ekaphan Parnmen, Sittiporn Luecking, Robert Plata, Eimy Rivas Aptroot, Andre Caceres, Marcela E. S. Ertz, Damien Mangold, Armin Mercado-Diaz, Joel A. Papong, Khwanruan Van den Broeck, Dries Weerakoon, Gothamie Lumbsch, H. Thorsten TI Revisiting the phylogeny of Ocellularieae, the second largest tribe within Graphidaceae (lichenized Ascomycota: Ostropales) SO PHYTOTAXA LA English DT Article DE Ampliotrema; Glaucotrema; phylogenetic resolution; Redingeria; Reimnitzia; Rhabdodiscus; Sarcographina; Stegobolus; supermatrix ID RIBOSOMAL DNA; LECANOROMYCETES OSTROPALES; THELOTREMOID GRAPHIDACEAE; FAMILY THELOTREMATACEAE; MOLECULAR PHYLOGENY; SUBUNIT; FUNGI; AUSTRALIA; INFERENCE; ALIGNMENT AB We present an updated 3-locus molecular phylogeny of tribe Ocellularieae, the second largest tribe within subfamily Graphidoideae in the Graphidaceae. Adding 165 newly generated sequences from the mitochondrial small subunit rDNA (mtSSU), the nuclear large subunit rDNA (nuLSU), and the second largest subunit of the DNA-directed RNA polymerase II (RPB2), we currently distinguish 218 species among the sequenced material, including the outgroup. This corresponds to almost half the species at this point recognized within this tribe. The newly generated sequences include 23 newly described species and one newly described genus published elsewhere in this volume. For the first time, Sarcographina cyclospora Mull. Arg., in spite of its distinctly lirellate ascomata, is shown to belong in tribe Ocellularieae, as strongly supported sister to Ocellularia inturgescens (Mull. Arg.) Mangold. The following six new combinations are proposed: Melanotrema lynceodes (Nyl.) Rivas Plata, Lucking & Lumbsch, Ocellularia curranii (Vain.) Kraichak, Lucking & Lumbsch, O. khasiana (Patw. & Nagarkar) Kraichak, Lucking & Lumbsch, O. cinerea (Mull. Arg.) Kraichak, Lucking & Lumbsch, O. erodens (R. C. Harris) Kraichak, Lucking & Lumbsch, and O. laeviuscula (Nyl) Kraichak, Lucking & Lumbsch. Further, the new name Ocellularia hernandeziana Kraichak, Lucking & Lumbsch is introduced for Myriotrema ecorticatum. The nomenclatural status of the name Ocellularia microstoma is clarified. C1 [Kraichak, Ekaphan; Luecking, Robert; Plata, Eimy Rivas; Lumbsch, H. Thorsten] Field Museum, Chicago, IL 60605 USA. [Parnmen, Sittiporn] Minist Publ Hlth, Dept Med Sci, Toxicol & Biochem Sect, Nonthaburi 11000, Thailand. [Aptroot, Andre] ABL Herbarium, NL-3762 XK Soest, Netherlands. [Caceres, Marcela E. S.] Univ Fed Sergipe, Dept Biociencias, BR-49500000 Itabaiana, Sergipe, Brazil. [Ertz, Damien] Bot Garden Meise, Dept Bryophytes Thallophytes, B-1860 Meise, Belgium. [Mangold, Armin] USDA Forest Serv, Int Inst Trop Forestry, Rio Piedras, PR USA. [Mercado-Diaz, Joel A.] Mahsarakham Univ, Fac Sci, Dept Biol, Kantarawichai 44150, Maha Sarakham P, Thailand. [Papong, Khwanruan] Univ Sri Jayawardenepura, Dept Bot, Nugegoda, Sri Lanka. RP Kraichak, E (reprint author), Field Museum, 1400 South Lake Shore Dr, Chicago, IL 60605 USA. EM sparnmen@gmail.com; rlucking@fieldmuseum.org; erivasplata@fieldmuseum.org; andreaptroot@gmail.com; mscaceres@hotmail.com; damien.ertz@br.fgov.be; arminmangold@gmail.com; joel_pr19@hotmail.com; khwanruanpapong@gmail.com; dries.van.den.broeck@br.fgov.be; gothamiew@yahoo.com; tlumbsch@fieldmuseum.org RI Caceres, Marcela/H-7039-2012 FU National Science Foundation: TICOLICHEN [DEB 0206125]; Phylogeny and Taxonomy of Ostropalean Fungi; Emphasis on the Lichen-forming Thelotremataceae [DEB 0516116]; Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms [DEB 715660]; ATM-Assembling a taxonomic monograph: The lichen family Graphidaceae [DEB-1025861] FX Data obtained for this study were gathered as part of several projects funded by the National Science Foundation: TICOLICHEN (DEB 0206125 to The Field Museum; PI Robert Lucking), Phylogeny and Taxonomy of Ostropalean Fungi, with Emphasis on the Lichen-forming Thelotremataceae (DEB 0516116 to The Field Museum; PI H. T. Lumbsch; Co-PI R. Lucking), Neotropical Epiphytic Microlichens-An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms (DEB 715660 to The Field Museum; PI R. Lucking), and ATM-Assembling a taxonomic monograph: The lichen family Graphidaceae (DEB-1025861 to The Field Museum; PI T. Lumbsch, CoPI R. Lucking). Fred Barrie is thanked for nomenclatural advice. NR 83 TC 18 Z9 18 U1 1 U2 1 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD DEC 19 PY 2014 VL 189 IS 1 SI SI BP 52 EP 81 PG 30 WC Plant Sciences SC Plant Sciences GA AY4LH UT WOS:000347549100004 ER PT J AU Mercado-Diaz, JA Lucking, R Parnmen, S AF Mercado-Diaz, Joel A. Luecking, Robert Parnmen, Sittiporn TI Two new genera and twelve new species of Graphidaceae from Puerto Rico: a case for higher endemism of lichenized fungi in islands of the Caribbean? SO PHYTOTAXA LA English DT Article DE Caribbean; conservation ID ASCOMYCOTA OSTROPALES; THELOTREMOID GRAPHIDACEAE; AGARICALES HYGROPHORACEAE; FOREST; CLADE; KEY; DICTYONEMA; PHYLOGENY; HISTORY AB Two new genera and twelve new species of Graphidaceae are described from Puerto Rico. The two new genera, Borinquenotrema and Paratopeliopsis, are based on a combination of molecular sequence data and phenotype characters. Borinquenotrema, with the single new species B. soredicarpum, features rounded ascomata developing beneath and persistently covered with soralia and with an internal anatomy reminescent of Carbacanthographis; it is close to the tribe Ocellularieae. Paratopeliopsis, including the single new species P. caraibica, resembles a miniature Topeliopsis but differs in the distinctly farinose thallus and the small, brown ascospores; it is not closely related to the latter genus but belongs in tribe Thelotremateae. The other ten new species belong in the genera Acanthotrema, Clandestinotrema, Compositrema, Fissurina, Ocellularia, and Thalloloma. Acanthotrema alboisidiatum is closely related to A. brasilianum but differs in the short, white isidia resembling insect eggs. Clandestinotrema portoricense has a unique ascospore type with a longitudinal septum only in the proximal cell. Compositrema borinquense resembles a species of Stegobolus but belongs in Compositrema based on sequence data, and is characterized by ascomata with a unique columella composed of thick, irregularly radiating strands. The second new species in this genus, C. isidiofarinosum, differs by its ecorticate, farinose thallus with scattered, corticate isidia and by its small ascomata with inconspicuous columella. The three new species of Fissurina all have 3-septate ascospores and are otherwise characterized by an isidiate thallus and stellate, orange-yellow lirellae (F. aurantiacostellata), a verrucose thallus strongly encrusted with calcium oxalate crystals and white, irregularly branched lirellae (F. crystallifera), and myriotremoid ascomata arranged in short lines (F. monilifera). Ocellularia portoricensis belongs in the core group of Ocellularia and differs from O. cavata in the white medulla and the larger ascospores becoming brown, whereas O. vulcanisorediata produces prominent soralia and immersed ascomata with apically carbonized excipulum and columella and small, transversely septate, hyaline ascospores; it is closely related to O. conformalis. Finally, Thalloloma rubromarginatum resembles T. haemographum in the brownish lirellae with bright red margin but differs from that and other species in the corticate thallus and the norstictic acid chemistry. The new combination Ampliotrema rimosum (Hale) Mercado-Diaz, Lucking & Parnmen is also proposed. Considering the current biodiversity knowledge on this family, the high level of endemism observed in other groups of organisms in the island, and the relatively high number of Graphidaceae described, it is highly likely that at least some of these new taxa are endemic to the island. This view is further supported by the unique features of several of the new species, representing novel characters in the corresponding genera. C1 [Mercado-Diaz, Joel A.] US Forest Serv, Int Inst Trop Forestry, USDA, Rio Piedras, PR 00926 USA. [Luecking, Robert] Field Museum, Chicago, IL 60605 USA. [Parnmen, Sittiporn] Minist Publ Hlth, Dept Med Sci, Toxicol & Biochem Sect, Nonthaburi 11000, Thailand. RP Mercado-Diaz, JA (reprint author), US Forest Serv, Int Inst Trop Forestry, USDA, Ceiba St 1201, Rio Piedras, PR 00926 USA. EM joel_pr19@hotmail.com; rlucking@fieldmuseum.org; sparnmen@gmail.com FU IITF State and Private Forestry Program; National Science Foundation [DEB 0206125, DEB 0516116, DEB 715660, DEB-1025861] FX Data obtained for this study were gathered as part of the following initiatives: the project Characterizing lichen communities along an elevational gradient in Puerto Rico: Assessing their role as indicators of forest health, biodiversity and microclimate lead by the first author and William A Gould (IITF) and supported by the IITF State and Private Forestry Program, and the following projects funded by the National Science Foundation: TICOLICHEN (DEB 0206125 to The Field Museum; PI Robert Lucking), Phylogeny and Taxonomy of Ostropalean Fungi, with Emphasis on the Lichen-forming Thelotremataceae (DEB 0516116 to The Field Museum; PI H. T. Lumbsch; Co-PI R. Lucking), Neotropical Epiphytic Microlichens - An Innovative Inventory of a Highly Diverse yet Little Known Group of Symbiotic Organisms (DEB 715660 to The Field Museum; PI R. Lucking), and ATM - Assembling a taxonomic monograph: The lichen family Graphidaceae (DEB-1025861 to The Field Museum; PI T. Lumbsch, CoPI R. Lucking). Dr. Grizelle Gonzalez and Maria Rivera (IITF) provided access and valuable information related to collection sites. Dr. D. Jean Lodge (Northern Research Station, FS) provided insightful comments to the manuscript. The curators at herbaria from which type specimens and other collections were studied are thanked for their collaboration. All research at the International Institute of Tropical Forestry is done in collaboration with the University of Puerto Rico. NR 63 TC 7 Z9 7 U1 0 U2 3 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD DEC 19 PY 2014 VL 189 IS 1 SI SI BP 186 EP 203 PG 18 WC Plant Sciences SC Plant Sciences GA AY4LH UT WOS:000347549100012 ER PT J AU Flower, A Gavin, DG Heyerdahl, EK Parsons, RA Cohn, GM AF Flower, Aquila Gavin, Daniel G. Heyerdahl, Emily K. Parsons, Russell A. Cohn, Gregory M. TI Western Spruce Budworm Outbreaks Did Not Increase Fire Risk over the Last Three Centuries: A Dendrochronological Analysis of Inter-Disturbance Synergism SO PLOS ONE LA English DT Article ID MOUNTAIN PINE-BEETLE; TREE-RING RECONSTRUCTION; ACTIVE CROWN FIRE; SUB-ALPINE FOREST; BRITISH-COLUMBIA; LODGEPOLE PINE; ROCKY-MOUNTAINS; UNITED-STATES; NORTH-AMERICA; PACIFIC-NORTHWEST AB Insect outbreaks are often assumed to increase the severity or probability of fire occurrence through increased fuel availability, while fires may in turn alter susceptibility of forests to subsequent insect outbreaks through changes in the spatial distribution of suitable host trees. However, little is actually known about the potential synergisms between these natural disturbances. Assessing inter-disturbance synergism is challenging due to the short length of historical records and the confounding influences of land use and climate changes on natural disturbance dynamics. We used dendrochronological methods to reconstruct defoliator outbreaks and fire occurrence at ten sites along a longitudinal transect running from central Oregon to western Montana. We assessed synergism between disturbance types, analyzed long-term changes in disturbance dynamics, and compared these disturbance histories with dendroclimatological moisture availability records to quantify the influence of moisture availability on disturbances. After approximately 1890, fires were largely absent and defoliator outbreaks became longer-lasting, more frequent, and more synchronous at our sites. Fires were more likely to occur during warm-dry years, while outbreaks were most likely to begin near the end of warm-dry periods. Our results show no discernible impact of defoliation events on subsequent fire risk. Any effect from the addition of fuels during defoliation events appears to be too small to detect given the overriding influence of climatic variability. We therefore propose that if there is any relationship between the two disturbances, it is a subtle synergistic relationship wherein climate determines the probability of occurrence of each disturbance type, and each disturbance type damps the severity, but does not alter the probability of occurrence, of the other disturbance type over long time scales. Although both disturbance types may increase in frequency or extent in response to future warming, our records show no precedent that western spruce budworm outbreaks will increase future fire risk. C1 [Flower, Aquila; Gavin, Daniel G.] Univ Oregon, Dept Geog, Eugene, OR 97403 USA. [Heyerdahl, Emily K.; Parsons, Russell A.; Cohn, Gregory M.] US Forest Serv, Rocky Mt Res Stn, USDA, Missoula, MT 59808 USA. RP Flower, A (reprint author), Univ Oregon, Dept Geog, 1251 Univ Oregon, Eugene, OR 97403 USA. EM Aquila.Flower@wwu.edu RI Gavin, Daniel/C-9214-2009 OI Gavin, Daniel/0000-0001-8743-3949 FU United States Department of Agriculture Joint Fire Science Program [09-1-06-5]; Mazamas Foundation; Doctoral Dissertation Research Improvement grant from the National Science Foundation [1233278]; Rocky Mountain Research Station (Forest Service, U.S. Department of Agriculture) [09-JV-11221637]; University of Oregon FX This research was supported in part by funds provided by the United States Department of Agriculture Joint Fire Science Program as Project 09-1-06-5 (firescience.gov); funding for AF, DG, EH, RP, and GC. AF was also funded through a Graduate Student Research Grant from the Mazamas Foundation (mazamasfoundation.org) and a Doctoral Dissertation Research Improvement grant (#1233278) from the National Science Foundation (nsf.gov). This research was undertaken with support from the Rocky Mountain Research Station (Forest Service, U.S. Department of Agriculture) under Research Joint Venture Agreement 09-JV-11221637 and the University of Oregon. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 86 TC 3 Z9 3 U1 2 U2 39 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 19 PY 2014 VL 9 IS 12 AR e114282 DI 10.1371/journal.pone.0114282 PG 25 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX8WK UT WOS:000347186200004 PM 25526633 ER PT J AU Li, ZD Yao, CH Wang, F Cai, ZY Wang, XD AF Li, Zhaodong Yao, Chunhua Wang, Fei Cai, Zhiyong Wang, Xudong TI Cellulose nanofiber-templated three-dimension TiO2 hierarchical nanowire network for photoelectrochemical photoanode SO NANOTECHNOLOGY LA English DT Article DE atomic layer deposition; cellulose nanofiber; 3D structure ID PERFORMANCE; ELECTRODES; NANOMATERIALS; ARCHITECTURE; ARRAYS AB Three dimensional (3D) nanostructures with extremely large porosity possess a great promise for the development of high-performance energy harvesting and storage devices. In this paper, we developed a high-density 3D TiO2 fiber-nanorod (NR) heterostructure for efficient photoelectrochemical (PEC) water splitting. The hierarchical structure was synthesized on a ZnO-coated cellulose nanofiber (CNF) template using atomic layer deposition (ALD)-based thin film and NR growth procedures. The tubular structure evolution was in good agreement with the recently discovered vapor-phase Kirkendall effect in high-temperature ALD processes. The NR morphology was formed via the surface-reaction-limited pulsed chemical vapor deposition (SPCVD) mechanism. Under Xenon lamp illumination without and with an AM 1.5G filter or a UV cut off filter, the PEC efficiencies of a 3D TiO2 fiber-NR heterostructure were found to be 22-249% higher than those of the TiO2-ZnO bilayer tubular nanofibers and TiO2 nanotube networks that were synthesized as reference samples. Such a 3D TiO2 fiber-NR heterostructure offers a new route for a cellulose-based nanomanufacturing technique, which can be used for large-area, low-cost, and green fabrication of nanomaterials as well as their utilizations for efficient solar energy harvesting and conversion. C1 [Li, Zhaodong; Yao, Chunhua; Wang, Fei; Wang, Xudong] Univ Wisconsin, Dept Mat Sci & Engn, Madison, WI 53706 USA. [Wang, Xudong] Chinese Acad Sci, Beijing Inst Nanoenergy & Nanosyst, Beijing 100083, Peoples R China. [Cai, Zhiyong] USDA, Forest Prod Lab, Forest Serv, Madison, WI 53726 USA. RP Li, ZD (reprint author), Univ Wisconsin, Dept Mat Sci & Engn, 1509 Univ Ave, Madison, WI 53706 USA. EM zcai@fs.fed.us; xudong@engr.wisc.edu RI Wang, Fei/E-7918-2013; Wang, Xudong/A-7067-2009 FU US Department of Energy (DOE), Office of Science, Basic Energy Sciences (BES) [DE-SC0008711]; National Science Foundation [CMMI-1233570] FX Research was primarily supported by the US Department of Energy (DOE), Office of Science, Basic Energy Sciences (BES), under Award # DE-SC0008711. CY thanks the support of the National Science Foundation under Award CMMI-1233570. NR 37 TC 7 Z9 8 U1 13 U2 105 PU IOP PUBLISHING LTD PI BRISTOL PA TEMPLE CIRCUS, TEMPLE WAY, BRISTOL BS1 6BE, ENGLAND SN 0957-4484 EI 1361-6528 J9 NANOTECHNOLOGY JI Nanotechnology PD DEC 19 PY 2014 VL 25 IS 50 AR 504005 DI 10.1088/0957-4484/25/50/504005 PG 10 WC Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied SC Science & Technology - Other Topics; Materials Science; Physics GA AU4TI UT WOS:000345603900005 PM 25426973 ER PT J AU Malone, SL Staudhammer, CL Oberbauer, SF Olivas, P Ryan, MG Schedlbauer, JL Loescher, HW Starr, G AF Malone, Sparkle L. Staudhammer, Christina L. Oberbauer, Steven F. Olivas, Paulo Ryan, Michael G. Schedlbauer, Jessica L. Loescher, Henry W. Starr, Gregory TI El Nino Southern Oscillation (ENSO) Enhances CO2 Exchange Rates in Freshwater Marsh Ecosystems in the Florida Everglades SO PLOS ONE LA English DT Article ID CARBON SEQUESTRATION; FLUX MEASUREMENTS; PRECIPITATION; PATTERNS; TEMPERATURE; ANOMALIES; VEGETATION; FREQUENCY; PHENOLOGY; EXTREMES AB This research examines the relationships between El Nino Southern Oscillation (ENSO), water level, precipitation patterns and carbon dioxide (CO2) exchange rates in the freshwater wetland ecosystems of the Florida Everglades. Data was obtained over a 5-year study period (2009-2013) from two freshwater marsh sites located in Everglades National Park that differ in hydrology. At the short-hydroperiod site (Taylor Slough; TS) and the long-hydroperiod site (Shark River Slough; SRS) fluctuations in precipitation patterns occurred with changes in ENSO phase, suggesting that extreme ENSO phases alter Everglades hydrology which is known to have a substantial influence on ecosystem carbon dynamics. Variations in both ENSO phase and annual net CO2 exchange rates co-occurred with changes in wet and dry season length and intensity. Combined with site-specific seasonality in CO2 exchanges rates, El Nino and La Nina phases magnified season intensity and CO2 exchange rates at both sites. At TS, net CO2 uptake rates were higher in the dry season, whereas SRS had greater rates of carbon sequestration during the wet season. As La Nina phases were concurrent with drought years and extended dry seasons, TS became a greater sink for CO2 on an annual basis (-11 to -110 g CO2 m(-2) yr(-1)) compared to El Nino and neutral years (-5 to -43.5 g CO2 m(-2) yr(-1)). SRS was a small source for CO2 annually (1.81 to 80 g CO2 m(-2) yr(-1)) except in one exceptionally wet year that was associated with an El Nino phase (-16 g CO2 m(-2) yr(-1)). Considering that future climate predictions suggest a higher frequency and intensity in El Nino and La Nina phases, these results indicate that changes in extreme ENSO phases will significantly alter CO2 dynamics in the Florida Everglades. C1 [Malone, Sparkle L.; Staudhammer, Christina L.; Starr, Gregory] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Malone, Sparkle L.; Ryan, Michael G.] US Forest Serv, Rocky Mt Res Stn, Ft Collins, CO USA. [Olivas, Paulo; Schedlbauer, Jessica L.] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA. [Ryan, Michael G.] Colorado State Univ, Nat Resource Ecol Lab, Ft Collins, CO 80523 USA. [Schedlbauer, Jessica L.] W Chester Univ, Dept Biol, W Chester, PA 19380 USA. [Loescher, Henry W.] Natl Ecol Observ Network Inc, Boulder, CO 80301 USA. [Loescher, Henry W.] Univ Colorado, Inst Arctic & Alpine Res, Boulder, CO 80309 USA. RP Malone, SL (reprint author), Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. EM sparklelmalone@fs.fed.us RI Ryan, Michael/A-9805-2008 OI Ryan, Michael/0000-0002-2500-6738 FU National Science Foundations (NSF) Division of Atmospheric and Geospace Sciences (AGS) Atmospheric Chemistry program [1233006]; Department of Energy's (DOE) National Institute for Climate Change Research (NICCR) [07-SC-NICCR-1059]; US Department of Education Graduate Assistantships in Areas of National Need (GAANN); National Science Foundation (NSF) through the Florida Coastal Everglades Long Term Ecological Research program under Cooperative Agreements [DBI-0620409, DEB-9910514] FX Funding for the research was provided by the National Science Foundations (NSF) Division of Atmospheric and Geospace Sciences (AGS) Atmospheric Chemistry program through grant 1233006 [GS SFO], the Department of Energy's (DOE) National Institute for Climate Change Research (NICCR) through grant 07-SC-NICCR-1059 [GS SFO], and the US Department of Education Graduate Assistantships in Areas of National Need (GAANN) grant [SLM]. This research was also supported by the National Science Foundation (NSF) through the Florida Coastal Everglades Long Term Ecological Research program under Cooperative Agreements DBI-0620409 and DEB-9910514 [GS SFO]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 65 TC 3 Z9 3 U1 2 U2 19 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 18 PY 2014 VL 9 IS 12 AR e115058 DI 10.1371/journal.pone.0115058 PG 30 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA4AC UT WOS:000348845600015 PM 25521299 ER PT J AU Verocai, GG Kutz, SJ Simard, M Hoberg, EP AF Verocai, Guilherme G. Kutz, Susan J. Simard, Manon Hoberg, Eric P. TI Varestrongylus eleguneniensis sp n. (Nematoda: Protostrongylidae): a widespread, multi-host lungworm of wild North American ungulates, with an emended diagnosis for the genus and explorations of biogeography SO PARASITES & VECTORS LA English DT Article DE Alces americanus; Cervidae; Nearctic; Rangifer tarandus; Ovibos moschatus; Taxonomy; Varestrongylinae; Verminous pneumonia ID MOOSE ALCES-ALCES; CARIBOU RANGIFER-TARANDUS; WHITE-TAILED DEER; UMINGMAKSTRONGYLUS-PALLIKUUKENSIS NEMATODA; PARELAPHOSTRONGYLUS-ANDERSONI NEMATODA; ELAPHOSTRONGYLUS CERVI INFECTION; OVIBOS-MOSCHATUS; MITOCHONDRIAL PHYLOGEOGRAPHY; ODOCOILEUS-VIRGINIANUS; MUSKOXEN AB Background: A putative new species of Varestrongylus has been recently recognized in wild North American ungulates based on the ITS-2 sequences of larvae isolated from feces during a wide geographic survey. No taxonomic description was provided, as adult specimens were not examined. Methods: Lungworm specimens were collected in the terminal bronchioles of muskoxen from Quebec, and a woodland caribou from central Alberta, Canada. The L3 stage was recovered from experimentally infected slugs (Deroceras spp.). Description of specimens was based on comparative morphology and integrated approaches. Molecular identity was determined by PCR and sequencing of the ITS-2 region of the nuclear ribosomal DNA, and compared to other protostrongylids. Results: Varestrongylus eleguneniensis sp. n. is established for a recently discovered protostrongylid nematode found in caribou (Rangifer tarandus), muskoxen (Ovibos moschatus) and moose (Alces americanus); hosts that collectively occupy an extensive geographic range across northern North America. Adults of Varestrongylus eleguneniensis are distinguished from congeners by a combination of characters in males (distally bifurcate gubernaculum, relatively short equal spicules not split distally, a strongly elongate and bifurcate dorsal ray, and an undivided copulatory bursa) and females (reduced provagina with hood-like fold extending ventrally across prominent genital protuberance). Third-stage larvae resemble those found among other species in the genus. The genus Varestrongylus is emended to account for the structure of the dorsal ray characteristic of V. eleguneniensis, V. alpenae, V. alces and V. longispiculatus. Conclusions: Herein we describe and name V. eleguneniensis, a pulmonary protostrongylid with Rangifer tarandus as a primary definitive host, and which secondarily infects muskoxen and moose in areas of sympatry. Biogeographic history for V. eleguneniensis and V. alpenae, the only two endemic species of Varestrongylus known from North America, appears consistent with independent events of geographic expansion with cervid hosts from Eurasia into North America during the late Pliocene and Quaternary. C1 [Verocai, Guilherme G.; Kutz, Susan J.] Univ Calgary, Dept Ecosyst & Publ Hlth, Fac Vet Med, Calgary, AB T2N 4Z6, Canada. [Kutz, Susan J.] Univ Calgary, Canadian Wildlife Hlth Cooperat Alberta Node, Fac Vet Med, Calgary, AB T2N 4N1, Canada. [Simard, Manon] Makivik Corp, Nunavik Res Ctr, Kuujjuaq, PQ J0M 1C0, Canada. [Hoberg, Eric P.] ARS, US Natl Parasite Collect, USDA, Beltsville, MD 20705 USA. RP Verocai, GG (reprint author), Univ Calgary, Dept Ecosyst & Publ Hlth, Fac Vet Med, 3280 Hosp Dr NW, Calgary, AB T2N 4Z6, Canada. EM gverocai@gmail.com FU Faculty of Veterinary Medicine of the University of Calgary; Alberta Innovates Health Solutions; Alberta Conservation Association - Grants in Biodiversity; W. Garfield Weston/Wildlife Conservation Society Canada Fellowship for Northern Conservation; CircumArctic Rangifer Monitoring and Assessment Network (CARMA); NSERC Canada International Polar Year Funding; Alberta Innovates and NSERC Discovery; Northern Supplement; Research Tools and Instruments grants; Beringian Coevolution Project (DEB-Biotic Surveys and Inventory) [0415668]; National Science Foundation; E. P. Hoberg (USNPC) FX This research is part of G. Verocai's PhD Thesis, and was supported by the Faculty of Veterinary Medicine of the University of Calgary, Alberta Innovates Health Solutions, Alberta Conservation Association - Grants in Biodiversity, The W. Garfield Weston/Wildlife Conservation Society Canada Fellowship for Northern Conservation, and the CircumArctic Rangifer Monitoring and Assessment Network (CARMA, www.carmanetwork.com), NSERC Canada International Polar Year Funding, and partially funded by Alberta Innovates and NSERC Discovery, Northern Supplement, and Research Tools and Instruments grants secured by S.J. Kutz; the Beringian Coevolution Project (DEB-Biotic Surveys and Inventory-0415668) with funding from the National Science Foundation to J. A. Cook (University of New Mexico) and E. P. Hoberg (USNPC). Our study was completed through the Integrated Inventory of Biomes of the Arctic (NSF, DEB-Biodiversity Discovery and Analysis - 1258010) to J. A. Cook, E. P. Hoberg, K. E. Galbreath (Northern Michigan University) and E. Dechaine (Western Washington University). The authors thank the Nunavik Research Centre, Makivik Corporation, especially Bill Doidge, FranCois Martin, Peter May, Mishal Naseer, and the hunters from Tasiujaq; Dr. Margo Pybus and Bob McClymont and, Alberta Fish and Wildlife Division; Patricia Pilitt and Art Abrams from the USNPC, USDA; Drs. Alexander Eberhardt, and John Gilleard (UCVM); the Kutz lab members: Jessica Ouellet, Jayninn Yue, Dean Brown, Jesse Invik, Manigandan Lejeune and James Wang. Likewise, we would like to acknowledge the crew involved in the trip to communities in the Sahtu Settlement Area, NT in search for the species name: Alasdair M. Veitch and Richard Popko, Department of Environment and Natural Resources, Government of the Northwest Territories, and Dr. Cyntia K. Kashivakura (UCVM). We specially acknowledge the communities in the Sahtu Settlement Area, NT: Bruce Kenny and Verna Firth, and the Kenny family from Deline; Rodger Boniface, Angus Shae, Wilfred Jackson, Michel Lafferty, and George Voudrak (Renewable Resources Committee) from Fort Good Hope; Joseph and Wilbert Kochon, Barry Gully (Behdzi Ahda First Nation), and the elders Mary Rose Drybones (translator), John Gully, Hyacinth, Marie and Antoine Kochon, and Johnny Blancho from Colville Lake, for providing input on naming of the parasite and Dora Grandjambe for assisting with enquiries on the North Slavey language. NR 82 TC 1 Z9 2 U1 3 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD DEC 17 PY 2014 VL 7 AR 556 DI 10.1186/s13071-014-0556-9 PG 22 WC Parasitology SC Parasitology GA CC6GX UT WOS:000350464500001 PM 25518883 ER PT J AU Dhillon, B Gill, N Hamelin, RC Goodwin, SB AF Dhillon, Braham Gill, Navdeep Hamelin, Richard C. Goodwin, Stephen B. TI The landscape of transposable elements in the finished genome of the fungal wheat pathogen Mycosphaerella graminicola SO BMC GENOMICS LA English DT Article ID INDUCED POINT MUTATION; DE-NOVO IDENTIFICATION; MAGNAPORTHE-GRISEA; NEUROSPORA-CRASSA; ILLEGITIMATE RECOMBINATION; TELOMERE MAINTENANCE; SEPTORIA-TRITICI; CHROMOSOME ENDS; MAIZE GENOME; DNA LOSS AB Background: In addition to gene identification and annotation, repetitive sequence analysis has become an integral part of genome sequencing projects. Identification of repeats is important not only because it improves gene prediction, but also because of the role that repetitive sequences play in determining the structure and evolution of genes and genomes. Several methods using different repeat-finding strategies are available for whole-genome repeat sequence analysis. Four independent approaches were used to identify and characterize the repetitive fraction of the Mycosphaerella graminicola (synonym Zymoseptoria tritici) genome. This ascomycete fungus is a wheat pathogen and its finished genome comprises 21 chromosomes, eight of which can be lost with no obvious effects on fitness so are dispensable. Results: Using a combination of four repeat-finding methods, at least 17% of the M. graminicola genome was estimated to be repetitive. Class I transposable elements, that amplify via an RNA intermediate, account for about 70% of the total repetitive content in the M. graminicola genome. The dispensable chromosomes had a higher percentage of repetitive elements as compared to the core chromosomes. Distribution of repeats across the chromosomes also varied, with at least six chromosomes showing a non-random distribution of repetitive elements. Repeat families showed transition mutations and a CpA -> TpA dinucleotide bias, indicating the presence of a repeat-induced point mutation (RIP)-like mechanism in M. graminicola. One gene family and two repeat families specific to subtelomeres also were identified in the M. graminicola genome. A total of 78 putative clusters of nested elements was found in the M. graminicola genome. Several genes with putative roles in pathogenicity were found associated with these nested repeat clusters. This analysis of the transposable element content in the finished M. graminicola genome resulted in a thorough and highly curated database of repetitive sequences. Conclusions: This comprehensive analysis will serve as a scaffold to address additional biological questions regarding the origin and fate of transposable elements in fungi. Future analyses of the distribution of repetitive sequences in M. graminicola also will be able to provide insights into the association of repeats with genes and their potential role in gene and genome evolution. C1 [Dhillon, Braham; Hamelin, Richard C.] Dept Forest & Conservat Sci, Vancouver, BC V6T 1Z4, Canada. [Gill, Navdeep] Beaty Biodivers Ctr, Dept Bot, Vancouver, BC V6T 1Z4, Canada. [Hamelin, Richard C.] Laurentian Forestry Ctr, Nat Resources Canada, Quebec City, PQ G1V 4C7, Canada. [Goodwin, Stephen B.] Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, W Lafayette, IN 47907 USA. RP Goodwin, SB (reprint author), Purdue Univ, USDA ARS, Crop Prod & Pest Control Res Unit, 915 W State St, W Lafayette, IN 47907 USA. EM sgoodwin@purdue.edu OI Goodwin, Stephen/0000-0001-5708-9729 FU USDA CRIS project [3602-22000-015-00D]; Genome Canada Large Scale Applied Research Project [164DIA] FX DNA sequencing of M. graminicola was performed at the U.S. Department of Energy's Joint Genome Institute through the Community Sequencing Program (www.jgi.doe.gov/csp/) and all sequence data are publicly available. Supported by USDA CRIS project 3602-22000-015-00D. BD and RCH were supported by Genome Canada Large Scale Applied Research Project 164DIA. NR 80 TC 14 Z9 14 U1 3 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD DEC 17 PY 2014 VL 15 AR 1132 DI 10.1186/1471-2164-15-1132 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA CB8CA UT WOS:000349854700001 PM 25519841 ER PT J AU Verocai, GG Hoberg, EP Vikoren, T Handeland, K Ytrehus, B Rezansoff, AM Davidson, RK Gilleard, JS Kutz, SJ AF Verocai, Guilherme G. Hoberg, Eric P. Vikoren, Turid Handeland, Kjell Ytrehus, Bjornar Rezansoff, Andrew M. Davidson, Rebecca K. Gilleard, John S. Kutz, Susan J. TI Resurrection and redescription of Varestrongylus alces (Nematoda: Protostrongylidae), a lungworm of the Eurasian moose (Alces alces), with report on associated pathology SO PARASITES & VECTORS LA English DT Article DE Cervidae; Cryptic species; Historical biogeography; ITS-2; Metastrongyloidea; Parasite biodiversity; Varestrongylinae; Varestrongylus capreoli; Verminous pneumonia ID ELAPHOSTRONGYLUS CERVI INFECTION; INTERNAL TRANSCRIBED SPACER; UMINGMAKSTRONGYLUS-PALLIKUUKENSIS; MITOCHONDRIAL PHYLOGEOGRAPHY; HAEMONCHUS-CONTORTUS; RANGE EXPANSION; NORTH-AMERICA; RUMINANTS; PARASITE; TRICHOSTRONGYLIDAE AB Background: Varestrongylus alces, a lungworm in Eurasian moose from Europe has been considered a junior synonym of Varestrongylus capreoli, in European roe deer, due to a poorly detailed morphological description and the absence of a type-series. Methods: Specimens used in the redescription were collected from lesions in the lungs of Eurasian moose, from Vestby, Norway. Specimens were described based on comparative morphology and integrated approaches. Molecular identification was based on PCR, cloning and sequencing of the ITS-2 region of the nuclear ribosomal DNA. Phylogenetic analysis compared Varestrongylus alces ITS-2 sequences to these of other Varestrongylus species and other protostrongylids. Results: Varestrongylus alces is resurrected for protostrongylid nematodes of Eurasian moose from Europe. Varestrongylus alces causes firm nodular lesions that are clearly differentiated from the adjacent lung tissue. Histologically, lesions are restricted to the parenchyma with adult, egg and larval parasites surrounded by multinucleated giant cells, macrophages, eosinophilic granulocytes, lymphocytes. The species is valid and distinct from others referred to Varestrongylus, and should be separated from Varestrongylus capreoli. Morphologically, Varestrongylus alces can be distinguished from other species by characters in the males that include a distally bifurcated gubernaculum, arched denticulate crura, spicules that are equal in length and relatively short, and a dorsal ray that is elongate and bifurcated. Females have a well-developed provagina, and are very similar to those of Varestrongylus capreoli. Morphometrics of first-stage larvae largely overlap with those of other Varestrongylus. Sequences of the ITS-2 region strongly support mutual independence of Varestrongylus alces, Varestrongylus cf. capreoli, and the yet undescribed species of Varestrongylus from North American ungulates. These three taxa form a well-supported crown-clade as the putative sister of Varestrongylus alpenae. The association of Varestrongylus alces and Alces or its ancestors is discussed in light of host and parasite phylogeny and host historical biogeography. Conclusions: Varestrongylus alces is a valid species, and should be considered distinct from Varestrongylus capreoli. Phylogenetic relationships among Varestrongylus spp. from Eurasia and North America are complex and consistent with faunal assembly involving recurrent events of geographic expansion, host switching and subsequent speciation. C1 [Verocai, Guilherme G.; Kutz, Susan J.] Univ Calgary, Fac Vet Med, Dept Ecosyst & Publ Hlth, Calgary, AB T2N 4Z6, Canada. [Hoberg, Eric P.] USDA ARS, US Natl Parasite Collect, Beltsville, MD 20705 USA. [Vikoren, Turid; Handeland, Kjell; Ytrehus, Bjornar; Davidson, Rebecca K.] Norwegian Vet Inst, N-0454 Oslo, Norway. [Rezansoff, Andrew M.; Gilleard, John S.] Univ Calgary, Fac Vet Med, Dept Comparat Biol & Expt Med, Calgary, AB T2N 4N1, Canada. [Kutz, Susan J.] Univ Calgary, Canadian Wildlife Hlth Cooperat Alberta Node, Fac Vet Med, Calgary, AB T2N 4Z6, Canada. RP Verocai, GG (reprint author), Univ Calgary, Fac Vet Med, Dept Ecosyst & Publ Hlth, 3280 Hosp Dr NW, Calgary, AB T2N 4Z6, Canada. EM gverocai@gmail.com FU Faculty of Veterinary Medicine, University of Calgary; Alberta Innovates Health Solutions; NSERC CREATE Host-Parasite Interactions; National Health Surveillance Program for Cervids (HOP) at NVI; Alberta Innovates; NSERC Discovery Grant; National Science Foundation; Integrated Inventory of Biomes of the Arctic (NSF, DEB- Biodiversity Discovery and Analysis) [1258010] FX This research is part of GV's PhD Thesis, and was supported by the Faculty of Veterinary Medicine, University of Calgary; Alberta Innovates Health Solutions; and NSERC CREATE Host-Parasite Interactions. This work was partially funded by the National Health Surveillance Program for Cervids (HOP) at NVI, Alberta Innovates and NSERC Discovery Grant secured by S.J. Kutz; the Beringian Coevolution Project (DEB- Biotic Surveys and Inventory- 0415668) with funding from the National Science Foundation to J. A. Cook (University of New Mexico) and E. P. Hoberg (USNPC). Our study received partial support from the Integrated Inventory of Biomes of the Arctic (NSF, DEB- Biodiversity Discovery and Analysis - 1258010) to J. A. Cook, E. P. Hoberg, K. E. Galbreath (Northern Michigan University) and E. Dechaine (Western Washington University). The authors are thankful to Vidar Holthe (Vestby), Lena Haugland Moen and Inger Sofie Hamnes (NVI), Dr. Mariana S. Panayotova-Pencheva (Institute of Experimental Morphology, Pathology and Anthropology, Bulgarian Academy of Sciences), Kim Koczka and James Wang (UCVM), Patricia Pilitt and Art Abrams (USNPC, USDA), and Dr. Arseny Makarikov (Institute of Systematics and Ecology of Animals, Siberian Branch, Russian Academy of Sciences). NR 70 TC 2 Z9 5 U1 7 U2 19 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD DEC 17 PY 2014 VL 7 AR 557 DI 10.1186/s13071-014-0557-8 PG 21 WC Parasitology SC Parasitology GA CB1SQ UT WOS:000349408700001 PM 25518921 ER PT J AU Webb, KJ Cookson, A Allison, G Sullivan, ML Winters, AL AF Webb, K. Judith Cookson, Alan Allison, Gordon Sullivan, Michael L. Winters, Anal L. TI Polyphenol oxidase affects normal nodule development in red clover (Trifolium pratense L.) SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE RNAi transformants; red clover; polyphenol oxidase (PPO); nodule; antioxidant activity; phenolics; Raman microspectrometry ID LEGUME ROOT-NODULES; REACTIVE OXYGEN; DIFFERENTIAL EXPRESSION; RHIZOBIUM-LEGUMINOSARUM; ANTIOXIDANT DEFENSES; NITROGEN-FIXATION; INDOLEACETIC-ACID; MULTIGENE FAMILY; STRESS TOLERANCE; SOYBEAN NODULES AB Polyphenol oxidase (PPO) may have multiple functions in tissues depending on its cellular or tissue localization. Here we use PPO RNAi transformants of red clover (Trifolium pratense) to determine the role PPO plays in normal development of plants, and especially in N-2-fixing nodules. In red clover, PPO was not essential for either growth or nodule production, or for nodule function in plants grown under optimal, N-free conditions. However, absence of PPO resulted in a more reduced environment in all tissues, as measured by redox potential, and caused subtle developmental changes in nodules. Leaves and, to a lesser extent nodules, lacking PPO tended to accumulate phenolic compounds. A comparison of nodules of two representative contrasting clones by microscopy revealed that nodules lacking PPO were morphologically and anatomically subtly altered, and that phenolics accumulated in different cells and tissues. Developing nodules lacking PPO were longer, and there were more cell layers within the squashed cell layer (SCL), but the walls of these cells were less thickened and the cells were less squashed. Within the N-2-fixing zone, bacteroids appeared more granular and were less tightly packed together, and were similar to developmentally compromised bacteroids elicited by catalase mutant rhizobia reported elsewhere C1 [Webb, K. Judith; Cookson, Alan; Allison, Gordon; Winters, Anal L.] Aberystwyth Univ, Inst Biol Environm & Rural Sci, Aberystwyth, Dyfed, Wales. [Sullivan, Michael L.] ARS, US Dairy Forage Res Ctr, USDA, Madison, WI USA. RP Webb, KJ (reprint author), Aberystwyth Univ, Inst Biol Environm & Rural Sci, Gogerddan SY23 3EE, Ceredigion, England. EM jxw@aber.ac.uk OI Sullivan, Michael/0000-0002-8517-4493 FU Biotechnology, Biological Sciences Research Council UK; European Regional Development Fund; Welsh European Funding Office FX We thank Ruth Sanderson for statistical advice. This research is funded by the Biotechnology, Biological Sciences Research Council UK and the European Regional Development Fund through funding provided for the BEACON project by the Welsh European Funding Office. NR 54 TC 2 Z9 2 U1 3 U2 27 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD DEC 17 PY 2014 VL 5 AR 700 DI 10.3389/fpls.2014.00700 PG 14 WC Plant Sciences SC Plant Sciences GA AY8BG UT WOS:000347778800001 PM 25566275 ER PT J AU Poulose, SM Bielinski, DF Carrihill-Knoll, KL Rabin, BM Shukitt-Hale, B AF Poulose, Shibu M. Bielinski, Donna F. Carrihill-Knoll, Kirsty L. Rabin, Bernard M. Shukitt-Hale, Barbara TI Protective effects of blueberry- and strawberry diets on neuronal stress following exposure to Fe-56 particles SO BRAIN RESEARCH LA English DT Article DE Neurochemical; Autophagy; Irradiation; Oxidative stress; Inflammation ID LONG-TERM POTENTIATION; FE-56-PARTICLE RADIATION; HIPPOCAMPAL NEUROGENESIS; OXIDATIVE STRESS; RAT HIPPOCAMPUS; GENE-EXPRESSION; BRAIN; AUTOPHAGY; IRRADIATION; DEFICITS AB Particles of high energy and charge (HZE particles), which are abundant outside the magnetic field of the Earth, have been shown to disrupt the functioning of neuronal communication in critical regions of the brain. Previous studies with HZE particles, have shown that irradiation produces enhanced indices of oxidative stress and inflammation as well as altered neuronal function that are similar to those seen in aging. Feeding animals antioxidant-rich berry diets, specifically blueberries and strawberries, countered the deleterious effects of irradiation by reducing oxidative stress and inflammation, thereby improving neuronal signaling. In the current study, we examined the effects of exposure to Fe-56 particles in critical regions of brain involved in cognitive function, both 36 h and 30 days post irradiation. We also studied the effects of antioxidant-rich berry diets, specifically a 2% blueberry or strawberry diet, fed for 8 weeks prior to radiation as well as 30 days post irradiation. Fe-56 exposure caused significant differential, neurochemical changes in critical regions of the brain, such as hippocampus, striatum, frontal cortex, and cerebellum, through increased inflammation, and increased oxidative stress protein markers. Fe-56 exposure altered the autophagy markers, and antioxidant-rich berry diets significantly reduced the accumulation of p62 in hippocampus, a scaffold protein that co-localizes with ubiquitinated protein at the 30 days post irradiation time-point. Exposure to Fe-56 particles increased the accumulation of disease-related proteins such as PHF-tau in the hippocampus of animals fed the control diet, but not in the irradiated animals fed the blueberry diet. These results indicate the potential protective effects of antioxidant-rich berry diets on neuronal functioning following exposure to HZE particles. Published by Elsevier B.V. C1 [Poulose, Shibu M.; Bielinski, Donna F.; Shukitt-Hale, Barbara] USDA ARS, Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Carrihill-Knoll, Kirsty L.; Rabin, Bernard M.] UMBC, Dept Psychol, Baltimore, MD 21250 USA. RP Shukitt-Hale, B (reprint author), Tufts Univ, USDA ARS, Human Nutr Res Ctr Aging, Neurosci & Aging Lab, 711 Washington St, Boston, MA 02111 USA. EM barbara.shukitthale@ars.usda.gov FU USDA Intramural; N.A.S.A [NNX08AM66G, NNX13AB73G] FX This research was supported in part by USDA Intramural and N.A.S.A. Grants NNX08AM66G and NNX13AB73G. The authors would like to thank the personnel of the Animal Care Facilities of BNL and the HNRCA for their assistance in caring for the rats. NR 52 TC 12 Z9 12 U1 1 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0006-8993 EI 1872-6240 J9 BRAIN RES JI Brain Res. PD DEC 17 PY 2014 VL 1593 BP 9 EP 18 DI 10.1016/j.brainres.2014.10.028 PG 10 WC Neurosciences SC Neurosciences & Neurology GA AX5FJ UT WOS:000346951900002 PM 25451098 ER PT J AU Zelinka, SL AF Zelinka, Samuel L. TI Preserving ancient artifacts for the next millennia SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Editorial Material ID WOOD C1 Forest Serv, Forest Prod Lab, USDA, Madison, WI 53715 USA. RP Zelinka, SL (reprint author), Forest Serv, Forest Prod Lab, USDA, Bldg & Fire Sci, Madison, WI 53715 USA. EM szelinka@fs.fed.us NR 13 TC 0 Z9 0 U1 0 U2 5 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD DEC 16 PY 2014 VL 111 IS 50 BP 17700 EP 17701 DI 10.1073/pnas.1420835111 PG 2 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AW6GA UT WOS:000346366500029 PM 25489087 ER PT J AU Walsh, Z Janecek, ER Hodgkinson, JT Sedlmair, J Koutsioubas, A Spring, DR Welch, M Hirschmugl, CJ Toprakcioglu, C Nitschke, JR Jones, M Scherman, OA AF Walsh, Zarah Janecek, Emma-Rose Hodgkinson, James T. Sedlmair, Julia Koutsioubas, Alexandros Spring, David R. Welch, Martin Hirschmugl, Carol J. Toprakcioglu, Chris Nitschke, Jonathan R. Jones, Mark Scherman, Oren A. TI Multifunctional supramolecular polymer networks as next-generation consolidants for archaeological wood conservation SO PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA LA English DT Article DE supramolecular polymer; conservation; waterlogged archaeological wood; Mary Rose ID SWEDISH WARSHIP VASA; FOCAL-PLANE ARRAY; POLYETHYLENE-GLYCOL; NEUTRON-SCATTERING; MOLECULAR-WEIGHT; MARY-ROSE; DEGRADATION; COMPLEXES; CHITOSAN; SULFUR AB The preservation of our cultural heritage is of great importance to future generations. Despite this, significant problems have arisen with the conservation of waterlogged wooden artifacts. Three major issues facing conservators are structural instability on drying, biological degradation, and chemical degradation on account of Fe3+- catalyzed production of sulfuric and oxalic acid in the waterlogged timbers. Currently, no conservation treatment exists that effectively addresses all three issues simultaneously. A new conservation treatment is reported here based on a supramolecular polymer network constructed from natural polymers with dynamic cross-linking formed by a combination of both host-guest complexation and a strong siderophore pendant from a polymer backbone. Consequently, the proposed consolidant has the ability to chelate and trap iron while enhancing structural stability. The incorporation of antibacterial moieties through a dynamic covalent linkage into the network provides the material with improved biological resistance. Exploiting an environmentally compatible natural material with completely reversible chemistries is a safer, greener alternative to current strategies and may extend the lifetime of many culturally relevant waterlogged artifacts around the world. C1 [Walsh, Zarah; Janecek, Emma-Rose; Scherman, Oren A.] Univ Cambridge, Dept Chem, Melville Lab Polymer Synth, Cambridge CB2 1EW, England. [Hodgkinson, James T.; Spring, David R.; Nitschke, Jonathan R.] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England. [Hodgkinson, James T.; Welch, Martin] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England. [Sedlmair, Julia] USDA, US Forest Serv, Forest Prod Lab, Madison, WI 53276 USA. [Sedlmair, Julia] Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA. [Sedlmair, Julia; Hirschmugl, Carol J.] Univ Wisconsin, Ctr Synchrotron Radiat, Stoughton, WI 53589 USA. [Koutsioubas, Alexandros] Forschungszentrum Julich, Heinz Maier Leibnitz Zentrum, Julich Ctr Neutron Sci, D-85747 Garching, Germany. [Hirschmugl, Carol J.] Univ Wisconsin, Dept Phys, Milwaukee, WI 53211 USA. [Toprakcioglu, Chris] Univ Patras, Dept Phys, Patras 26500, Greece. [Jones, Mark] HM Naval Base, Mary Rose Trust, Portsmouth PO1 3LX, Hants, England. RP Scherman, OA (reprint author), Univ Cambridge, Dept Chem, Melville Lab Polymer Synth, Lensfield Rd, Cambridge CB2 1EW, England. EM oas23@cam.ac.uk RI Koutsioumpas, Alexandros/L-3555-2013; Nitschke, Jonathan/A-1664-2010; Spring, David/K-2283-2016 OI Koutsioumpas, Alexandros/0000-0001-9417-5108; Walsh, Zarah/0000-0001-8570-045X; Nitschke, Jonathan/0000-0002-4060-5122; Spring, David/0000-0001-7355-2824 FU European Research Council Starting Investigator Grant [240629]; Walters-Kundert Charitable Trust; Mary Rose Trust; National Science Foundation (NSF) Award [MRI-0619759]; NSF [CHE-1112433]; University of Wisconsin-Madison; University of Wisconsin-Milwaukee; US Forest Service and Forest Products Laboratory (Madison, WI) FX We thank Dr. D. Reid for help in optimizing the ssNMR experiments. This work was supported by an European Research Council Starting Investigator Grant [Aqueous Supramolecular Polymers and Peptide Conjugates in Reversible Systems (ASPiRe), Grant 240629], a Next Generation fellowship provided by the Walters-Kundert Charitable Trust, an Engineering and Physical Sciences Research Council PhD stipend for E.-R. J., and the Mary Rose Trust. Part of this work is based on research conducted at the Infrared Environmental Imaging (IRENI) beamline whose construction and development was supported by National Science Foundation (NSF) Award MRI-0619759. This work was supported by NSF Grant CHE-1112433. The Synchrotron Radiation Center is primarily funded by the University of Wisconsin-Madison, with supplemental support from facility users and the University of Wisconsin-Milwaukee. This research was supported in part by the US Forest Service and Forest Products Laboratory (Madison, WI). NR 40 TC 4 Z9 4 U1 3 U2 41 PU NATL ACAD SCIENCES PI WASHINGTON PA 2101 CONSTITUTION AVE NW, WASHINGTON, DC 20418 USA SN 0027-8424 J9 P NATL ACAD SCI USA JI Proc. Natl. Acad. Sci. U. S. A. PD DEC 16 PY 2014 VL 111 IS 50 BP 17743 EP 17748 DI 10.1073/pnas.1406037111 PG 6 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AW6GA UT WOS:000346366500037 PM 25385610 ER PT J AU Kaufman, Z Abler, D Shortle, J Harper, J Hamlett, J Feather, P AF Kaufman, Zach Abler, David Shortle, James Harper, Jayson Hamlett, James Feather, Peter TI Agricultural Costs of the Chesapeake Bay Total Maximum Daily Load SO ENVIRONMENTAL SCIENCE & TECHNOLOGY LA English DT Article AB This study estimates costs to agricultural producers of the Watershed Implementation Plans (WIPs) developed by states in the Chesapeake Bay Watershed to comply with the Chesapeake Bay total maximum daily load (TMDL) and potential cost savings that could be realized by a more efficient selection of agricultural Best Management Practices (BMPs) and spatial targeting of BMP implementation. The cost of implementing the WIPs between 2011 and 2025 is estimated to be about $3.6 billion (in 2010 dollars). The annual cost associated with full implementation of all WIP BMPs from 2025 onward is about $900 million. Significant cost savings can be realized through careful and efficient BMP selection and spatial targeting. If retiring up to 25% of current agricultural land is included as an option, Bay-wide cost savings of about 60% could be realized compared to the WIPs. C1 [Kaufman, Zach; Harper, Jayson; Hamlett, James] Penn State Univ, University Pk, PA 16802 USA. [Shortle, James] Penn State Univ, Environm & Nat Resources Inst, University Pk, PA 16802 USA. [Feather, Peter] USDA, Agr Labor Affairs Coordinator Off, Washington, DC 20250 USA. EM dga2@psu.edu FU USDA Office of the Chief Economist [58-0111-11-006, 58-0111-13-014] FX We gratefully acknowledge funding from the USDA Office of the Chief Economist via Cooperative Agreement Nos. 58-0111-11-006 and 58-0111-13-014 for the study leading to this paper. Thanks are also extended to Abt Associates and the Chesapeake Bay Program for sharing their data and for their cooperation. NR 18 TC 2 Z9 2 U1 4 U2 27 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0013-936X EI 1520-5851 J9 ENVIRON SCI TECHNOL JI Environ. Sci. Technol. PD DEC 16 PY 2014 VL 48 IS 24 BP 14131 EP 14138 DI 10.1021/es502696t PG 8 WC Engineering, Environmental; Environmental Sciences SC Engineering; Environmental Sciences & Ecology GA AX1CO UT WOS:000346686100011 PM 25405490 ER PT J AU Cavagnaro, PF Iorizzo, M Yildiz, M Senalik, D Parsons, J Ellison, S Simon, PW AF Cavagnaro, Pablo F. Iorizzo, Massimo Yildiz, Mehtap Senalik, Douglas Parsons, Joshua Ellison, Shelby Simon, Philipp W. TI A gene-derived SNP-based high resolution linkage map of carrot including the location of QTL conditioning root and leaf anthocyanin pigmentation SO BMC GENOMICS LA English DT Article DE Carrot; Anthocyanins; QTL mapping; Linkage map; Single nucleotide polymorphism ID CAROTA L. GENOME; DAUCUS-CAROTA; GENOTYPING ERRORS; SWEET-POTATO; BIOSYNTHESIS; EXPRESSION; MARKERS; PURPLE; BIOAVAILABILITY; PATHWAY AB Background: Purple carrots accumulate large quantities of anthocyanins in their roots and leaves. These flavonoid pigments possess antioxidant activity and are implicated in providing health benefits. Informative, saturated linkage maps associated with well characterized populations segregating for anthocyanin pigmentation have not been developed. To investigate the genetic architecture conditioning anthocyanin pigmentation we scored root color visually, quantified root anthocyanin pigments by high performance liquid chromatography in segregating F-2, F-3 and F-4 generations of a mapping population, mapped quantitative trait loci (QTL) onto a dense gene-derived single nucleotide polymorphism (SNP)-based linkage map, and performed comparative trait mapping with two unrelated populations. Results: Root pigmentation, scored visually as presence or absence of purple coloration, segregated in a pattern consistent with a two gene model in an F2, and progeny testing of F-3-F-4 families confirmed the proposed genetic model. Purple petiole pigmentation was conditioned by a single dominant gene that co-segregates with one of the genes conditioning root pigmentation. Root total pigment estimate (RTPE) was scored as the percentage of the root with purple color. All five anthocyanin glycosides previously reported in carrot, as well as RTPE, varied quantitatively in the F2 population. For the purpose of QTL analysis, a high resolution gene-derived SNP-based linkage map of carrot was constructed with 894 markers covering 635.1 cM with a 1.3 cM map resolution. A total of 15 significant QTL for all anthocyanin pigments and for RTPE mapped to six chromosomes. Eight QTL with the largest phenotypic effects mapped to two regions of chromosome 3 with co-localized QTL for several anthocyanin glycosides and for RTPE. A single dominant gene conditioning anthocyanin acylation was identified and mapped. Comparative mapping with two other carrot populations segregating for purple color indicated that carrot anthocyanin pigmentation is controlled by at least three genes, in contrast to monogenic control reported previously. Conclusions: This study generated the first high resolution gene-derived SNP-based linkage map in the Apiaceae. Two regions of chromosome 3 with co-localized QTL for all anthocyanin pigments and for RTPE, largely condition anthocyanin accumulation in carrot roots and leaves. Loci controlling root and petiole anthocyanin pigmentation differ across diverse carrot genetic backgrounds. C1 [Cavagnaro, Pablo F.; Iorizzo, Massimo; Senalik, Douglas; Parsons, Joshua; Ellison, Shelby; Simon, Philipp W.] Univ Wisconsin, Dept Hort, Madison, WI 53706 USA. [Cavagnaro, Pablo F.] Univ Nacl Cuyo, CONICET, Fac Ciencias Agr, RA-5567 Mendoza, Argentina. [Cavagnaro, Pablo F.] INTA, EEA La Consulta, RA-5567 Mendoza, Argentina. [Yildiz, Mehtap] Yuzuncu Yil Univ, Fac Agr, Dept Agr Biotechnol, TR-65080 Van, Turkey. [Senalik, Douglas; Simon, Philipp W.] Univ Wisconsin, Vegetable Crops Unit, USDA Agr Res Serv, Madison, WI 53706 USA. RP Simon, PW (reprint author), Univ Wisconsin, Dept Hort, 1575 Linden Dr, Madison, WI 53706 USA. EM Philipp.Simon@ars.usda.gov FU Agricultural Research Service, United States Department of Agriculture FX The authors acknowledge Anne E. Atkins for valuable assistance on plant phenotyping and marker evaluation. This work was supported by the Agricultural Research Service, United States Department of Agriculture. NR 65 TC 2 Z9 2 U1 0 U2 37 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD DEC 16 PY 2014 VL 15 AR 1118 DI 10.1186/1471-2164-15-1118 PG 17 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AX3XY UT WOS:000346870200001 PM 25514876 ER PT J AU Vallet, JL McNeel, AK Miles, JR Freking, BA AF Vallet, Jeffrey L. McNeel, Anthony K. Miles, Jeremy R. Freking, Bradley A. TI Placental accommodations for transport and metabolism during intra-uterine crowding in pigs SO JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY LA English DT Review DE Glycosaminoglycans; Nutrient transport; Placenta ID AMINO-ACID TRANSPORTERS; FOLATE-BINDING-PROTEIN; UTERINE CAPACITY; OVULATION RATE; LITTER SIZE; FATTY-ACID; ZINC TRANSPORTER; IRON-METABOLISM; LATE-GESTATION; GRAVID UTERUS AB Litter size and birth weights are limited by uterine capacity, defined as the ability of the uterus to maintain the appropriate development of some number of conceptuses. Uterine capacity is the result of the combined effects of uterine, placental and embryo/fetal function. The number of living conceptuses that the uterus is capable of supporting is greater during early gestation compared to later gestation. Plots of log fetal weight versus log placental weight also indicate that fetal weights are less sensitive to reduced placental weight (and therefore reduced intrauterine space) in early gestation compared to late gestation. However, even in late gestation, mechanisms still exist that maintain fetal growth when the size of the placenta is reduced. One such mechanism is likely to be improved development of the folded placental-epithelial/maternal-epithelial bilayer. Fold depth, and therefore the maternal fetal interactive surface, increases as gestation advances and is greater in placenta from small fetuses. On the fetal side of the placenta, the epithelial bilayer is embedded in stromal tissue. Glycosaminoglycans are major components of stroma, including hyaluronan and heparan sulfate. Hyaluronidases and heparanases are present within placental tissues, and likely play roles in modification of stromal components to facilitate fold development. Glycosaminoglycans are polymers of forms of glucose (glucosamine, glucuronic acid, iduronic acid) suggesting that glycosaminoglycan synthesis may compete with the glucose needs of the developing fetus. Pig conceptuses are fructogenic, such that a substantial portion of glucose transferred from mother to fetus is converted to fructose. Fructose is an intermediate product in the synthesis of glucosamine from glucose, and glucosamine is linked to regulation of trophoblast cell proliferation through regulation of mTOR. These findings suggest a link between glucose, fructose, glucosamine synthesis, GAG production, and placental morphogenesis, but the details of these interactions remain unclear. In addition, recent placental epithelial transcriptome analysis identified several glucose, amino acid, lipid, vitamin, mineral and hormone transporter mechanisms within the placenta. Further elucidation of mechanisms of placental morphogenesis and solute transport could provide clues to improving nutrient transport to the pig fetus, potentially increasing litter size and piglet birth weights. C1 [Vallet, Jeffrey L.; McNeel, Anthony K.; Miles, Jeremy R.; Freking, Bradley A.] USDA ARS, US Meat Anim Res Ctr, Clay Ctr, NE 68933 USA. RP Vallet, JL (reprint author), USDA ARS, US Meat Anim Res Ctr, State Spur 18D, Clay Ctr, NE 68933 USA. EM jeff.vallet@ars.usda.gov NR 106 TC 3 Z9 3 U1 4 U2 12 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 2049-1891 J9 J ANIM SCI BIOTECHNO JI J. Anim. Sci. Biotechnol. PD DEC 15 PY 2014 VL 5 AR 55 DI 10.1186/2049-1891-5-55 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA CC9YT UT WOS:000350727800001 PM 25937925 ER PT J AU de Lucena, KFC Rodrigues, JMN Campos, EM Dantas, AFM Pfister, JA Cook, D Medeiros, RMT Riet-Correa, F AF Carvalho de Lucena, Kleber F. Rodrigues, Jussara M. N. Campos, Edipo M. Dantas, Antonio F. M. Pfister, James A. Cook, Daniel Medeiros, Rosane M. T. Riet-Correa, Franklin TI Poisoning by Ipomoea asartfolia in lambs by the ingestion of milk from ewes that ingest the plant SO TOXICON LA English DT Article DE Milk toxicity; Sheep; Toxic plants; Tremorgenic toxins ID ASARIFOLIA CONVOLVULACEAE; ERGOLINE ALKALOIDS; CLAVICEPS-PASPALI; GOATS; ASSOCIATION; BUFFALOS; STAGGERS; BRAZIL; SHEEP AB Two experiments, each with 10 pregnant ewes (8 treated and 2 controls) were performed to determine if nursing lambs of lactating ewes become intoxicated when the ewes ingest Ipomoea asanfolia but do not show clinical signs themselves. In the first experiment the sheep grazed I. asanfolia in the field while in the second, sheep were maintained in individual bays consuming dry I. asarifolia at 10% and 20% into their ration. In both experiments the lambs remained confined, consuming only their mother's milk. Four of 8 lambs in the grazing experiment and the 4 nursing lambs from the ewes given 20% L asarifolia showed signs of L asarifolia poisoning. These results confirm that the tremorgenic compound of L asarifolia or its toxic metabolites are eliminated in milk and can intoxicate nursing lambs. (C) 2014 Elsevier Ltd. All rights reserved. C1 [Carvalho de Lucena, Kleber F.; Rodrigues, Jussara M. N.; Campos, Edipo M.; Dantas, Antonio F. M.; Medeiros, Rosane M. T.; Riet-Correa, Franklin] Univ Fed Campina Grande, Vet Hosp, BR-58700310 Patos de Minas, Paraiba, Brazil. [Pfister, James A.; Cook, Daniel] USDA ARS, Poisonous Plant Res Lab, Logan, UT 84341 USA. RP Riet-Correa, F (reprint author), Univ Fed Campina Grande, Vet Hosp, BR-58700310 Patos de Minas, Paraiba, Brazil. EM rmtmed@uol.com.br; franklin.riet@pq.cnpq.br FU National Institute for Science and Technology for the Control of Plant Poisonings, CNPq [573534/2008-0] FX This work was supported by the National Institute for Science and Technology for the Control of Plant Poisonings, CNPq, grant 573534/2008-0. NR 20 TC 0 Z9 0 U1 2 U2 4 PU PERGAMON-ELSEVIER SCIENCE LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND SN 0041-0101 J9 TOXICON JI Toxicon PD DEC 15 PY 2014 VL 92 BP 129 EP 132 DI 10.1016/j.toxicon.2014.10.019 PG 4 WC Pharmacology & Pharmacy; Toxicology SC Pharmacology & Pharmacy; Toxicology GA AZ1RF UT WOS:000348014900019 ER PT J AU Baker, TP Jordan, GJ Steel, EA Fountain-Jones, NM Wardlaw, TJ Baker, SC AF Baker, Thomas P. Jordan, Gregory J. Steel, E. Ashley Fountain-Jones, Nicholas M. Wardlaw, Timothy J. Baker, Susan C. TI Microclimate through space and time: Microclimatic variation at the edge of regeneration forests over daily, yearly and decadal time scales SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Disturbance; Edge effects; Forest influence; Microclimate; Recolonisation; Variable retention ID DOUGLAS-FIR FORESTS; CLEAR-CUT EDGES; VARIABLE RETENTION; SOIL-MOISTURE; RAIN-FOREST; ECOLOGICAL BOUNDARIES; VEGETATION RESPONSES; WESTERN-AUSTRALIA; SPATIAL-PATTERNS; TEMPERATURE AB A major aim of sustainable forest management is the maintenance or recolonisation of harvested areas by species that were present pre-disturbance. Forest influence (a type of edge effect that focuses on the effect of mature forests on adjacent disturbed forest) is considered to be an important factor that contributes to the ability of mature forest species to re-colonise disturbed areas. Forest influence occurs in two main ways by: (1) by providing a source of propagules or individuals for recolonisation; and (2) by its influence on the biotic and abiotic conditions of the disturbed forest. This study focuses on forest influence's impact on microclimate conditions of adjacent disturbed areas regenerating after harvesting. In particular, the study investigates whether microclimate within a regenerating forest changes with increasing distance from a mature forest edge, and whether the magnitude of microclimatic change varies over diurnal, seasonal and successional time scales. Results of the study showed that the microclimate of regenerating forests is affected by the distance to a standing mature forest. Temperature, relative humidity, vapour pressure deficit, and the short-term fluctuations of these microclimate parameters were influenced by nearby mature forest. In addition, the study found that the magnitude of forest influence changes over diurnal, seasonal and successional time scales. For example, it was discovered that forest influence is greatest during the middle of the day, during the summer months when solar heating is greatest and on hot windy days. Critically; the impact of forest influence peaked around 27 years after disturbance in the areas studied, with less influence shortly after disturbance. We speculate this is due to lower levels of midday shading in the 7 year old forest. Forest influence on microclimate persisted in regeneration areas that were harvested 45 years ago, although the magnitude and importance of the effect was low. We conclude that proximity to mature forest stands (forests influence) impacts the microclimate of forests regenerating after disturbance, although the response is quite variable through time. Our results provide insight into the role of microclimate on the ability of mature forest species to successfully recolonise after disturbance. Management practices, such as aggregated retention and other forms of retention forestry, which increase the proportion of harvested area under forest influence, may provide a mechanism to promote the re-colonisation of mature-forest species. (C) 2014 Elsevier B.V. All rights reserved. C1 [Baker, Thomas P.; Jordan, Gregory J.; Fountain-Jones, Nicholas M.; Baker, Susan C.] Univ Tasmania, Sch Biol Sci, Hobart, Tas 7001, Australia. [Wardlaw, Timothy J.; Baker, Susan C.] Forestry Tasmania, Res & Dev Branch, Hobart, Tas, Australia. [Steel, E. Ashley] USDA, Pacific NW Res Stn, Forest Serv, Seattle, WA 98103 USA. RP Baker, TP (reprint author), Univ Tasmania, Sch Biol Sci, Private Bag 55, Hobart, Tas 7001, Australia. EM tpbaker@utas.edu.au OI Jordan, Greg/0000-0002-6033-2766 FU ARC Linkage Grant [LP100100050]; Forestry Tasmania; FFIC FX This project was supported by an ARC Linkage Grant LP100100050 with in kind support and funding provided by Forestry Tasmania and the FFIC. We thank Jayne Balmer for assistance in data collection, Dede Olson and the PNW research station for manuscript editing, and Robert Davies-Colley and anonymous reviewers for their constructive comments. NR 71 TC 11 Z9 12 U1 4 U2 38 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 15 PY 2014 VL 334 BP 174 EP 184 DI 10.1016/j.foreco.2014.09.008 PG 11 WC Forestry SC Forestry GA AY7KR UT WOS:000347739700018 ER PT J AU Gonzalez-Benecke, CA Gezan, SA Albaugh, TJ Allen, HL Burkhart, HE Fox, TR Jokela, EJ Maier, CA Martin, TA Rubilar, RA Samuelson, LJ AF Gonzalez-Benecke, Carlos A. Gezan, Salvador A. Albaugh, Timothy J. Allen, H. Lee Burkhart, Harold E. Fox, Thomas R. Jokela, Eric J. Maier, Chris A. Martin, Timothy A. Rubilar, Rafael A. Samuelson, Lisa J. TI Local and general above-stump biomass functions for loblolly pine and slash pine trees SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Pinus taeda; Pinus elliottii; Above ground allometry; Carbon stock modeling ID SOUTHERN UNITED-STATES; ESTIMATING FOREST BIOMASS; RADIATION-USE EFFICIENCY; BELOW-GROUND BIOMASS; UPPER COASTAL-PLAIN; ABOVEGROUND BIOMASS; STAND DEVELOPMENT; LEAF-AREA; GROWTH EFFICIENCY; ELLIOTTII STANDS AB There is an increasing interest in estimating biomass for loblolly pine (Pinus taeda L.) and slash pine (Pinus elliottii Engelm. var. elliottii), two of the most ecologically and commercially important tree species in North America. The majority of the available individual-tree allometric models are local, relying on stem diameter outside bark at breast height (dbh) and, in some cases, total tree height (H): only a few include stand age or other covariates. Using a large dataset collected from five forestry research institutions in the southeastern U.S., consisting of biomass measurements from 744 loblolly pine and 259 slash pine trees, we developed a set of individual-tree equations to predict total tree above-stump biomass, stem biomass outside bark, live branch biomass and live foliage biomass, as well as functions to determine stem bark fraction in order to calculate stem wood biomass inside bark and stem bark biomass from stem biomass outside bark determinations. Local and general models are presented for each tree attribute. Local models included dbh or dbh and H as predicting variables. General models included stand-level variables such as age, quadratic mean diameter, basal area and stand density. This paper reports the first set of local and general allometric equations reported for loblolly and slash pine trees. The models can be applied to trees growing over a large geographical area and across a wide range of ages and stand characteristics. These sets of equations provide a valuable alternative to available models and are intended as a tool to support present and future management decisions for the species, allowing for a variety of ecological, silvicultural and economic applications, as regional assessments of stand biomass or estimating ecosystem C balance. (C) 2014 Elsevier B.V. All rights reserved. C1 [Gonzalez-Benecke, Carlos A.; Gezan, Salvador A.; Jokela, Eric J.; Martin, Timothy A.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Albaugh, Timothy J.; Burkhart, Harold E.; Fox, Thomas R.] Virginia Polytech Inst & State Univ, Dept Forest Resources & Environm Conservat, Blacksburg, VA 24061 USA. [Allen, H. Lee] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Maier, Chris A.] USDA, Southern Res Stn, Forest Serv, Res Triangle Pk, NC 27709 USA. [Rubilar, Rafael A.] Univ Concepcion, Fac Ciencias Forest, Dept Silvicultura, Concepcion, Chile. [Samuelson, Lisa J.] Auburn Univ, Sch Forestry & Wildlife Sci, Auburn, AL 36849 USA. RP Gonzalez-Benecke, CA (reprint author), Univ Florida, Sch Forest Resources & Conservat, POB 110410, Gainesville, FL 32611 USA. EM cgonzabe@ufl.edu OI Martin, Timothy/0000-0002-7872-4194 FU U.S. Department of Defense, through the Strategic Environmental Research and Development Program (SERDP); University of Florida's Carbon Resources Science Center; Forest Biology Research Cooperative; USDA National Institute of Food and Agriculture, Coordinated Agricultural Project Award [2011-68002-30185] FX This research was supported by the U.S. Department of Defense, through the Strategic Environmental Research and Development Program (SERDP), the University of Florida's Carbon Resources Science Center, the Forest Biology Research Cooperative, and the USDA National Institute of Food and Agriculture, Coordinated Agricultural Project Award #2011-68002-30185. The authors acknowledge, with thanks, all individuals who contributed to collecting the biomass data used in this study. NR 134 TC 12 Z9 12 U1 5 U2 19 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 15 PY 2014 VL 334 BP 254 EP 276 DI 10.1016/j.foreco.2014.09.002 PG 23 WC Forestry SC Forestry GA AY7KR UT WOS:000347739700026 ER PT J AU West, DR Briggs, JS Jacobi, WR Negron, JF AF West, Daniel R. Briggs, Jennifer S. Jacobi, William R. Negron, Jose F. TI Mountain pine beetle-caused mortality over eight years in two pine hosts in mixed-conifer stands of the southern Rocky Mountains SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Forest disturbance; Dendroctonus ponderosae; Host selection; Hopkins' Host Selection Principle; Lodgepole pine; Ponderosa pine ID WESTERN UNITED-STATES; COLORADO FRONT RANGE; SPATIOTEMPORAL PATTERNS; TREE MORTALITY; LODGEPOLE PINE; PONDEROSA PINE; CLIMATE-CHANGE; BOREAL FOREST; BARK BEETLES; EXPANSION AB Eruptive mountain pine beetle (Dendroctonus ponderosae, MPB) populations have caused widespread mortality of pines throughout western North America since the late 1990s. Early work by AD. Hopkins suggested that when alternate host species are available, MPB will prefer to breed in the host to which it has become adapted. In Colorado, epidemic MPB populations that originated in lodgepole pine expanded into mixed-conifer stands containing ponderosa pine, a related host. We evaluated the susceptibility of both hosts to successful MPB colonization in a survey of 19 sites in pine-dominated mixed-conifer stands spanning 140 km of the Front Range, CO, USA. In each of three 0.2-ha plots at each site, we (1) assessed trees in the annual flights of 2008-2011 to compare MPB-caused mortality between lodgepole and ponderosa pine; (2) recorded previous MPB-caused tree mortality from 2004-2007 to establish baseline mortality levels; and (3) measured characteristics of the stands (e.g. tree basal area) and sites (e.g. elevation, aspect) that might be correlated with MPB colonization. Uninfested average live basal area of lodgepole and ponderosa pine was 74% of total basal area before 2004. We found that for both species, annual percent basal area of attacked trees was greatest in one year (2009), and was lower in all other years (2004-2007, 2008, 2010, and 2011). Both pine species had similar average total mortality of 38-39% by 2011. Significant predictors of ponderosa pine mortality in a given year were basal area of uninfested ponderosa pine and the previous year's mortality levels in both ponderosa and lodgepole pine. Lodgepole pine mortality was predicted by uninfested basal areas of both lodgepole and ponderosa pine, and the previous year's lodgepole pine mortality. These results indicate host selection by MPB from lodgepole pine natal hosts into ponderosa pine the following year, but not the reverse. In both species, diameters of attacked trees within each year were similar, and were progressively smaller the last four years of the study period. Our results suggest that, in contrast to previous reports, ponderosa and lodgepole pine were equally susceptible to MPB infestation in the CO Front Range during our study period. This suggests that forest managers may anticipate similar impacts in both hosts during similar environmental conditions when epidemic-level MPB populations are active in mixed-pine stands. (C) 2014 Elsevier B.V. All rights reserved. C1 [West, Daniel R.; Jacobi, William R.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Briggs, Jennifer S.] US Geol Survey, Geosci & Environm Change Sci Ctr, Denver, CO 80225 USA. [Negron, Jose F.] USDA, Forest Serv, Rocky Mt Res Stn, Ft Collins, CO 80525 USA. RP West, DR (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, C202 Plant Sci Bldg, Ft Collins, CO 80523 USA. EM drwest73@gmail.com FU US Geological Survey's Land Change Science Program in the Climate and Land Use Mission Area; Colorado State Forest Service; Boulder County Parks and Open Space; Colorado Agricultural Experiment Station FX This work was funded in part by the US Geological Survey's Land Change Science Program in the Climate and Land Use Mission Area, Colorado State Forest Service, Boulder County Parks and Open Space, and the Colorado Agricultural Experiment Station. We thank the US Forest Service, Arapaho-Roosevelt NF, the US National Park Service, Boulder County Parks and Open Space, and Colorado State Parks and Wildlife for access to sampling sites. We thank James zumBrunnen and the Franklin A. Graybill Statistical Laboratory, Colorado State University, for statistical consultation and guidance. We appreciate helpful comments from two anonymous journal reviewers, Mike Battaglia, and Dave Leatherman on a previous draft of the manuscript, and guidance from Craig Allen throughout the study. We thank the field technicians Kevin Miller, Zach Wehr, Jake Thomsen, Paul Cigan, Brison Bishop, Justin Pomeranz, Eric Eden, Tim Mapoles, Patrick Flynn, and Peter Pavlowich for tireless data collection and meticulous record keeping. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government. NR 44 TC 6 Z9 6 U1 0 U2 25 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 15 PY 2014 VL 334 BP 321 EP 330 DI 10.1016/j.foreco.2014.09.012 PG 10 WC Forestry SC Forestry GA AY7KR UT WOS:000347739700032 ER PT J AU Ager, AA Day, MA Finney, MA Vance-Borland, K Vaillant, NM AF Ager, Alan A. Day, Michelle A. Finney, Mark A. Vance-Borland, Ken Vaillant, Nicole M. TI Analyzing the transmission of wildfire exposure on a fire-prone landscape in Oregon, USA SO FOREST ECOLOGY AND MANAGEMENT LA English DT Article DE Wildfire simulation; Wildfire risk; Network analysis; Risk network; Forest fuel treatments ID FUEL TREATMENTS; WILDLAND FIRE; NETWORK ANALYSIS; SOCIAL NETWORKS; UNITED-STATES; RISK; FOREST; SIMULATION; CHALLENGES; MANAGEMENT AB We develop the idea of risk transmission from large wildfires and apply network analyses to understand its importance on a 0.75 million ha US national forest. Wildfires in the western US frequently burn over long distances (e.g., 20-50 km) through highly fragmented landscapes with respect to ownership, fuels, management intensity, population density, and ecological conditions. The collective arrangement of fuel loadings in concert with weather and suppression efforts ultimately determines containment and the resulting fire perimeter. While spatial interactions among land parcels in terms of fire spread and intensity have been frequently noted by fire managers, quantifying risk and exposure transmission has not been attempted. In this paper we used simulation modeling to quantify wildfire transmission and built a transmission network consisting of land designations defined by national forest management designations and ownership. We then examined how a forest-wide fuel management program might change the transmission network and associated metrics. The results indicated that the size, shape, and fuel loading of management designations affected their exposure to wildfire from other designations and ownerships. Manipulating the fuel loadings via simulated forest fuel treatments reduced the wildfire transmitted among the land designations, and changed the network density as well. We discuss how wildfire transmission has implications for creating fire adapted communities, conserving biodiversity, and resolving competing demands for fire-prone ecosystem services. (C) 2014 Elsevier B.V. All rights reserved. C1 [Ager, Alan A.; Vaillant, Nicole M.] USDA, Forest Serv, Pacific NW Res Stn, Western Wildland Environm Threat Assessment Ctr, Prineville, OR 97754 USA. [Day, Michelle A.; Vance-Borland, Ken] Oregon State Univ, Coll Forestry, Dept Forest Ecosyst & Soc, Corvallis, OR 97331 USA. [Finney, Mark A.] USDA, Forest Serv, Rocky Mt Res Stn, Fire Sci Lab, Missoula, MT 59808 USA. RP Ager, AA (reprint author), USDA, Forest Serv, Pacific NW Res Stn, Western Wildland Environm Threat Assessment Ctr, 3160 NE 3rd St, Prineville, OR 97754 USA. EM aager@fs.fed.us; michelle.day@oregonstate.edu; mfinney@fs.fed.us; kenvb@consplan.net; nvaillant@fs.fed.us NR 60 TC 10 Z9 10 U1 6 U2 24 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0378-1127 EI 1872-7042 J9 FOREST ECOL MANAG JI For. Ecol. Manage. PD DEC 15 PY 2014 VL 334 BP 377 EP 390 DI 10.1016/j.foreco.2014.09.017 PG 14 WC Forestry SC Forestry GA AY7KR UT WOS:000347739700037 ER PT J AU Jeong, J Kim, WH Yeo, J Fernandez, CP Kim, S Lee, YJ Lillehoj, HS Min, W AF Jeong, Jipseol Kim, Woo H. Yeo, Jaeseung Fernandez, Cherry P. Kim, Suk Lee, Youn-Jeong Lillehoj, Hyun S. Min, Wongi TI Identification of alternatively spliced isoforms of interleukin-2/15 receptor beta chain in ducks SO VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY LA English DT Article DE Duck; IL-2/1512 beta; Isoforms; Riemerella anatipestifer infection; gene expression ID NATURAL-KILLER-CELLS; MICE LACKING; IL-2; EXPRESSION; ACTIVATION; ASSOCIATION; GENERATION; JAK1 AB Interleukin (IL)-2 and IL-15 receptor 13 (IL-2/15R beta, CD122) play important roles in signal transduction for biological functions of IL-2 and IL-15. We found that ducks possess three different IL-2/15R beta transcripts, a conventional form (duIL-2/15R beta) and two variants. Comparisons between the cDNA and genomic sequences revealed that the two variants, duIL-2/15R beta-d7 and duIL-2/15R beta-d9, were novel spliced transcripts resulting from skipping exons 7 and 9, respectively. Expression profiles of duIL-2/15R beta and its isoforms were examined in healthy tissues, concanavalin A (ConA)-stimulated splenic lymphocytes and in livers and spleens of Riemerella anatipestifer-infected ducks using quantitative realtime PCR (qRT-PCR). Generally, duIL-2/15R beta-d9 expression was undetectable in healthy tissues, ConA-activated samples, and R. anatipestifer-infected ducks. Expression levels of duIL-2/15R beta transcript were relatively high to moderate in all healthy tissues tested, while duIL-2/15R13-d7 expression was low. Compared to untreated controls, expression levels of duIL-2/15R beta were elevated in ConA-activated splenic lymphocytes and in livers on day 7 in R. anatipestifer-infected ducks, while duIL-2/15R beta-d7 expression was unchanged. Additionally, COS-7 cells transfected with duIL-2/15R beta, duIL-2/15R beta-d7, or duIL-2/15R beta-d9 constructs generated 73 kilodalton (kDa), 31 kDa, and 40 kDa proteins, respectively. This study identified three different IL-2/15R beta transcripts, including two isoforms generated by alternative splicing and their gene expression patterns in stimulated conditions. (C) 2014 Elsevier B.V. All rights reserved. C1 [Jeong, Jipseol; Kim, Woo H.; Yeo, Jaeseung; Fernandez, Cherry P.; Kim, Suk; Min, Wongi] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, Gyeongnam, South Korea. [Jeong, Jipseol; Kim, Woo H.; Yeo, Jaeseung; Fernandez, Cherry P.; Kim, Suk; Min, Wongi] Gyeongsang Natl Univ, Inst Anim Med, Jinju 660701, Gyeongnam, South Korea. [Jeong, Jipseol; Lee, Youn-Jeong] Anim & Plant Quarantine Agcy, Avian Dis Div, Anyang 430757, South Korea. [Lillehoj, Hyun S.] ARS, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. RP Min, W (reprint author), Gyeongsang Natl Univ, Coll Vet Med, 501 Jinju Daero, Jinju 660701, Gyeongnam, South Korea. EM wongimin@gnu.ac.kr FU Basic Science Research Program [NRF-2013R1A1A4A01006646]; Cooperative Research Program for Agriculture Science & Technology Development [PJ008527032013]; RDA, Republic of Korea FX This research was supported by the Basic Science Research Program (NRF-2013R1A1A4A01006646), and by the Cooperative Research Program for Agriculture Science & Technology Development (PJ008527032013), RDA, Republic of Korea. NR 25 TC 2 Z9 2 U1 0 U2 3 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0165-2427 EI 1873-2534 J9 VET IMMUNOL IMMUNOP JI Vet. Immunol. Immunopathol. PD DEC 15 PY 2014 VL 162 IS 3-4 BP 154 EP 161 DI 10.1016/j.vetimm.2014.09.005 PG 8 WC Immunology; Veterinary Sciences SC Immunology; Veterinary Sciences GA AY7WY UT WOS:000347767800010 PM 25446847 ER PT J AU Lee, SH Lillehoj, HS Jang, SI Jeong, M Kim, DK Xu, S Lee, SK Kim, JB Park, HJ Kim, HR Bravo, DM AF Lee, Sung Hyen Lillehoj, Hyun S. Jang, Seung I. Jeong, Misun Kim, Duk Kyung Xu, Shouzhen Lee, Seung Kyu Kim, Jung Bong Park, Hong Ju Kim, Haeng Ran Bravo, David M. TI Immune and anti-oxidant effects of in ovo selenium proteinate on post-hatch experimental avian necrotic enteritis SO VETERINARY PARASITOLOGY LA English DT Article DE Selenium; Chicken; Anti-oxidant; Immunology; Necrotic enteritis ID MESSENGER-RNA EXPRESSION; EIMERIA-MAXIMA INFECTION; CLOSTRIDIUM-PERFRINGENS; BROILER-CHICKENS; OXIDATIVE STRESS; LIPID-PEROXIDATION; ALPHA-TOXIN; HOST; COCCIDIOSIS; SUPPLEMENTATION AB This study was conducted to investigate the effects of in ova administration of selenium (Se) incorporated into hydrolyzed soybean protein (B-Taxim [BT]) on protection against experimental avian necrotic enteritis (NE). Broiler eggs were injected with either 100 mu l of PBS alone (BT0), or 20 or 40 mu g/egg of BT in PBS (BT20, BT40) at 18 days of embryogenesis. On day 14 post-hatch, the chickens were uninfected or orally infected with 1.0 x 10(4) oocysts of Eimeria maxima (E. maxima). On day 18 post-hatch, E. maxima-infected chickens were orally infected with 1.0 x 10(9) CFU of Clostridium perfringens (C perfringens). Compared with untreated and infected BT0 controls, BT20 and/or BT40 birds showed increased body weights, decreased fecal shedding of E. maxima oocysts, lower serum a-toxin and NetB levels, increased levels of serum antibodies against C. perfringens a-toxin and NetB toxin, decreased levels of serum malondialdehyde, reduced serum catalase and superoxide dismutase catalytic activities, and increased intestinal levels of gene transcripts encoding interleukin (IL)-1 beta, IL-6, IL-8, and peroxiredoxin-6, but decreased levels of transcripts for catalase and glutathione peroxidase. Interestingly, transcript levels for inducible nitric oxide synthase and paraoxonase/arylesterase 2 were decreased in the BT20 group and increased in the BT40 group, compared with BT0 controls. These results indicate that in ova administration of broiler chickens with a Se-containing protein hydrolysate enhanced protection against experimental NE possibly by altering the expression of proinflammatory and anti-oxidant genes and their downstream pathways. Published by Elsevier B.V. C1 [Lee, Sung Hyen; Kim, Jung Bong; Park, Hong Ju; Kim, Haeng Ran] Natl Acad Agr Sci, Rural Dev Adm, Wanju Gun, Jeollabuk Do, South Korea. [Lillehoj, Hyun S.; Jang, Seung I.; Jeong, Misun; Lee, Seung Kyu] Agr Res Serv, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. [Kim, Duk Kyung] Hankyong Natl Univ, Anim Genom & Breeding Ctr, Ansong, Gyeonggi, South Korea. [Xu, Shouzhen] Qingdao Agr Univ, Coll Anim Sci & Vet Med, Qingdao, Peoples R China. [Bravo, David M.] Pancosma SA, Geneva, Switzerland. RP Lillehoj, HS (reprint author), Agr Res Serv, Anim Biosci & Biotechnol Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU ARS; USDA; Pancosma S.A.; Rural Development Administration, Republic of Korea [PJ009386] FX This study was partially supported by a formal Trust agreement established between ARS, USDA and Pancosma S.A. and by the project 'Anti-asthma effect of Salvia plebeia R. Br. and Adenophora triphylla var.japonica (PJ009386) of Rural Development Administration, Republic of Korea. The authors thank Dr. Kyung Woo Lee, Marjorie Nichols, and Stacy O'Donnell for their scientific input and technical assistance. NR 44 TC 1 Z9 1 U1 1 U2 12 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD DEC 15 PY 2014 VL 206 IS 3-4 BP 115 EP 122 DI 10.1016/j.vetpar.2014.10.025 PG 8 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AY7WP UT WOS:000347767000001 PM 25468015 ER PT J AU Jenkins, MC O'Brien, CN Fuller, L Mathis, GF Fetterer, R AF Jenkins, M. C. O'Brien, C. N. Fuller, L. Mathis, G. F. Fetterer, R. TI A rapid method for determining salinomycin and monensin sensitivity in Eimeria tenella SO VETERINARY PARASITOLOGY LA English DT Article DE Eimeria; lonophore; Culture; PCR; Drug sensitivity ID ANTICOCCIDIAL DRUGS; RESISTANCE; COCCIDIOSIS; FOWL; STRATEGIES; CHICKEN AB Standard methods of determining the ionophore sensitivity of Eimeria rely on infecting chickens with an isolate or a mixture of Eimeria spp. oocysts in the presence of different anti-coccidial drugs. The purpose of this study was to develop a rapid in vitro method for assessing salinomycin and monensin sensitivity in Eimeria tenella. Cultures of MDBK cells were grown to 85% confluency, and then inoculated with excysted E. tenella laboratory strain (APU-1) sporozoites in the presence of different concentrations of salinomycin or monensin. At various timepoints, the monolayers were fixed for counting intraceullar sporozoites, or were subjected to DNA extraction, followed by molecular analysis using quantitative (qPCR) or semi-quantitative PCR (sqPCR). Preliminary experiments showed that 24 h was the optimum time for harvesting the E. tene/la-infected cell cultures. The average number of E. tenella sporozoites relative to untreated controls displayed a linear decrease between 03 and 33.0 mu g/ml alinomycin and between 0.3 and 3.3 mu g/ml monensin. A similar pattern was observed in the relative amount of E. tenella DNA as measured by sqPCR. A linear decrease in the relative amount of E. tenella DNA was observed over the entire range of salinomycin and monensin concentrations as measured by qPCR possibly reflecting the greater sensitivity of this assay. Comparison of sporozoite counting, sqPCR, and qPCR signals using a criterion of 50% inhibition in sporozoite numbers or level of PCR amplification product showed good agreement between the three assays. E. tenella field isolates (FS-1 and FS-2) displaying resistance to salinomycin and monensin were evaluated in the in vitro assay using qPCR and sqPCR. Compared to E. tenella APU-1, the E. tenella FS-1 and FS-2 isolates showed higher levels of E. tenella DNA at 24 h by both qPCR and sciPCR. This in vitro assay represents a significant advance in developing rapid, cost-effective methods for assessing ionophore sensitivity in E. tenella. Published by Elsevier B.V. C1 [Jenkins, M. C.; O'Brien, C. N.; Fetterer, R.] Agr Res Serv, Anim Parasit Dis Lab, USDA, Beltsville, MD 20705 USA. [Fuller, L.] Univ Georgia, Dept Poultry Sci, Coll Agr & Environm Sci, Athens, GA 30602 USA. [Mathis, G. F.] Southern Poultry Res Inc, Athens, GA 30607 USA. RP Jenkins, MC (reprint author), Agr Res Serv, Anim Parasit Dis Lab, USDA, Bldg 1040, Beltsville, MD 20705 USA. EM mark.jenkins@ars.usda.gov FU USDA-ARS CRIS [1265-313200076-00D] FX The authors acknowledge the technical assistance of Ruth Barfield in preparation of E. tenella oocysts for use the in vitro assays. This project was solely funded by the USDA-ARS CRIS project "Development and Control of Intervention Strategies for Avian Coccidiosis" - Project No. 1265-313200076-00D. NR 18 TC 3 Z9 3 U1 1 U2 16 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD DEC 15 PY 2014 VL 206 IS 3-4 BP 153 EP 158 DI 10.1016/j.vetpar.2014.09.017 PG 6 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AY7WP UT WOS:000347767000006 PM 25312355 ER PT J AU Cabral, AD D'Auria, SRN Camargo, MCGO Rosa, AR Sodre, MM Galvao-Dias, MA Jordao, LR Dubey, J Gennari, SM Pena, HFJ AF Cabral, Aline D. D'Auria, Sandra R. N. Camargo, Maria C. G. O. Rosa, Adriana R. Sodre, Miriam M. Galvao-Dias, Maria A. Jordao, Luciana R. Dubey, J. P. Gennari, Solange M. Pena, Hilda F. J. TI Seroepidemiology of Toxoplasma gondii infection in bats from Sao Paulo city, Brazil SO VETERINARY PARASITOLOGY LA English DT Article DE Bats; Brazil; Serological survey; Toxoplasma gondii; Zoonoses; Wildlife ID PREVALENCE AB Toxoplasmosis is a parasitic zoonosis caused by Toxoplasma gondii protozoan with a worldwide distribution. The aim of this study was to determine the frequency of IgG anti-T. gondii antibodies in bats from Sao Paulo city, Brazil. A total of 616 serum samples were collected from 22 species of bats. Anti-T. gondii antibodies were searched using the indirect fluorescent antibody test (IFAT >= 1:16) and IgG anti-bat antibodies produced in sheep on samples collected during 2006-2011; 32.62% (201/616) of bats had T. gondii antibodies. The modified agglutination test (MAT >= 1:25) was performed on samples collected during 2010-2011; 18.61% (35/188) were seropositive. The concordance between IFAT and MAT (serum samples from 2010 to 2011) by Kappa (95% CI) was 0.144, resulting in a low agreement between the techniques. The specificity and sensitivity of MAT and IFAT have not been evaluated for the diagnosis of toxoplasmosis in bats. Thus, it was verified that bats are exposed to T. gondii during their lifetime and they are also part of the toxoplasmosis epidemiology. (C) 2014 Elsevier B.V. All rights reserved. C1 [Cabral, Aline D.; Gennari, Solange M.; Pena, Hilda F. J.] Univ Sao Paulo, Cidade Univ, Fac Med Vet Zootecnia, BR-05508270 Sao Paulo, Brazil. [D'Auria, Sandra R. N.; Camargo, Maria C. G. O.; Rosa, Adriana R.; Sodre, Miriam M.; Galvao-Dias, Maria A.; Jordao, Luciana R.] Ctr Controle Zoonoses, LabZoo, BR-02031020 Sao Paulo, Brazil. [Dubey, J. P.] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Pena, HFJ (reprint author), Univ Sao Paulo, Cidade Univ, Fac Med Vet Zootecnia, Av Prof Dr Orlando Marques Paiva,87, BR-05508270 Sao Paulo, Brazil. EM hfpena@usp.br FU CNPq [470180/2010-2]; FAPESP [2009/51889-2] FX The financial support for this work was provided by CNPq (470180/2010-2) and FAPESP (2009/51889-2). We thank volunteers and personnel from CCZ and USP who helped us to collect samples, processing and data. NR 22 TC 2 Z9 2 U1 0 U2 11 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 0304-4017 EI 1873-2550 J9 VET PARASITOL JI Vet. Parasitol. PD DEC 15 PY 2014 VL 206 IS 3-4 BP 293 EP 296 DI 10.1016/j.vetpar.2014.10.014 PG 4 WC Parasitology; Veterinary Sciences SC Parasitology; Veterinary Sciences GA AY7WP UT WOS:000347767000025 PM 25468027 ER PT J AU Sangild, PT Ney, DM Sigalet, DL Vegge, A Burrin, D AF Sangild, Per T. Ney, Denise M. Sigalet, David L. Vegge, Andreas Burrin, Douglas TI Animal models of gastrointestinal and liver diseases. Animal models of infant short bowel syndrome: translational relevance and challenges SO AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY LA English DT Article DE rat; mouse; pig; parenteral and enteral nutrition; glucagon-like peptide 2; newborn; intestine; resection ID GLUCAGON-LIKE PEPTIDE-2; TOTAL PARENTERAL-NUTRITION; SERIAL TRANSVERSE ENTEROPLASTY; CHAIN FATTY-ACIDS; GROWTH-FACTOR-I; NEONATAL PIGLET MODEL; ENHANCES INTESTINAL ADAPTATION; MASSIVE ILEOCECAL RESECTION; GENERATION LIPID EMULSIONS; MULTIPLE-VARIABLE ANALYSIS AB Intestinal failure (IF), due to short bowel syndrome (SBS), results from surgical resection of a major portion of the intestine, leading to reduced nutrient absorption and need for parenteral nutrition (PN). The incidence is highest in infants and relates to preterm birth, necrotizing enterocolitis, atresia, gastroschisis, volvulus, and aganglionosis. Patient outcomes have improved, but there is a need to develop new therapies for SBS and to understand intestinal adaptation after different diseases, resection types, and nutritional and pharmacological interventions. Animal studies are needed to carefully evaluate the cellular mechanisms, safety, and translational relevance of new procedures. Distal intestinal resection, without a functioning colon, results in the most severe complications and adaptation may depend on the age at resection (preterm, term, young, adult). Clinically relevant therapies have recently been suggested from studies in preterm and term PN-dependent SBS piglets, with or without a functional colon. Studies in rats and mice have specifically addressed the fundamental physiological processes underlying adaptation at the cellular level, such as regulation of mucosal proliferation, apoptosis, transport, and digestive enzyme expression, and easily allow exogenous or genetic manipulation of growth factors and their receptors (e.g., glucagon-like peptide 2, growth hormone, insulin-like growth factor 1, epidermal growth factor, keratinocyte growth factor). The greater size of rats, and especially young pigs, is an advantage for testing surgical procedures and nutritional interventions (e.g., PN, milk diets, long-/short-chain lipids, pre- and probiotics). Conversely, newborn pigs (preterm or term) and weanling rats provide better insights into the developmental aspects of treatment for SBS in infants owing to their immature intestines. The review shows that a balance among practical, economical, experimental, and ethical constraints will determine the choice of SBS model for each clinical or basic research question. C1 [Sangild, Per T.; Vegge, Andreas] Univ Copenhagen, Fac Sci, Dept Nutr Exercise & Sports, DK-1958 Frederiksberg C, Denmark. [Sangild, Per T.] Rigshosp, Dep Paediat & Adolescent Med, DK-2100 Copenhagen, Denmark. [Ney, Denise M.] Univ Wisconsin, Dept Nutr Sci, Madison, WI USA. [Sigalet, David L.] Sidra Med & Res Ctr, Doha, Qatar. [Vegge, Andreas] Novo Nordisk, Diabet Pharmacol, Malov, Denmark. [Burrin, Douglas] USDA ARS, Childrens Nutr Res Ctr, Baylor Coll Med, Houston, TX USA. RP Sangild, PT (reprint author), Univ Copenhagen, Fac Sci, Dept Nutr Exercise & Sports, DK-1958 Frederiksberg C, Denmark. EM psa@life.ku.dk OI Sangild, Per Torp/0000-0002-5462-7760 FU UNIK program at University of Copenhagen; NEOMUNE grant from the Danish Research Councils; federal funds from the USDA Agricultural Research Service [58-6250-6-001]; National Institutes of Health [DK-094616, DK-042835]; Texas Medical Center Digestive Diseases Center (National Institutes of Health) [P30 DK-56338]; Alberta Children's Hospital Research Foundation; Sidra Medical and Research Centre Research Foundation FX The work was supported in part by the UNIK program at University of Copenhagen, NEOMUNE grant from the Danish Research Councils, federal funds from the USDA Agricultural Research Service under Cooperative Agreement Number 58-6250-6-001, National Institutes of Health Grants DK-094616 and DK-042835, the Texas Medical Center Digestive Diseases Center (National Institutes of Health Grant P30 DK-56338), Alberta Children's Hospital Research Foundation, and Sidra Medical and Research Centre Research Foundation. NR 278 TC 9 Z9 9 U1 0 U2 26 PU AMER PHYSIOLOGICAL SOC PI BETHESDA PA 9650 ROCKVILLE PIKE, BETHESDA, MD 20814 USA SN 0193-1857 EI 1522-1547 J9 AM J PHYSIOL-GASTR L JI Am. J. Physiol.-Gastroint. Liver Physiol. PD DEC 15 PY 2014 VL 307 IS 12 BP G1147 EP G1168 DI 10.1152/ajpgi.00088.2014 PG 22 WC Gastroenterology & Hepatology; Physiology SC Gastroenterology & Hepatology; Physiology GA AW8AC UT WOS:000346482000002 PM 25342047 ER PT J AU Xie, J Sun, G Chu, HS Liu, JG McNulty, SG Noormets, A John, R Ouyang, ZT Zha, TS Li, HT Guan, WB Chen, JQ AF Xie, Jing Sun, Ge Chu, Hou-Sen Liu, Junguo McNulty, Steven G. Noormets, Asko John, Ranjeet Ouyang, Zutao Zha, Tianshan Li, Haitao Guan, Wenbin Chen, Jiquan TI Long-term variability in the water budget and its controls in an oak-dominated temperate forest SO HYDROLOGICAL PROCESSES LA English DT Article DE evapotranspiration; water budget; interannual and seasonal variability; climatic warming; eddy-covariance; temperate deciduous forest ID EDDY-COVARIANCE; SAP-FLOW; NORTHERN WISCONSIN; FLUX MEASUREMENTS; SONIC ANEMOMETER; MANAGED FORESTS; ENERGY-EXCHANGE; USE EFFICIENCY; CARBON FLUXES; EVAPOTRANSPIRATION AB Water availability is one of the key environmental factors that control ecosystem functions in temperate forests. Changing climate is likely to alter the ecohydrology and other ecosystem processes, which affect forest structures and functions. We constructed a multi-year water budget (2004-2010) and quantified environmental controls on an evapotranspiration (ET) in a 70-year-old mixed-oak woodland forest in northwest Ohio, USA. ET was measured using the eddy-covariance technique along with precipitation (P), soil volumetric water content (VWC), and shallow groundwater table fluctuation. Three biophysical models were constructed and validated to calculate potential ET (PET) for developing predictive monthly ET models. We found that the annual variability in ET was relatively stable and ranged from 578mm in 2009 to 670mm in 2010. In contrast, ET/P was more variable and ranged from 0.60 in 2006 to 0.96 in 2010. Mean annual ET/PET_FAO was 0.64, whereas the mean annual PET_FAO/P was 1.15. Annual ET/PET_FAO was relatively stable and ranged from 0.60 in 2005 to 0.72 in 2004. Soil water storage and shallow groundwater recharge during the non-growing season were essential in supplying ET during the growing season when ET exceeded P. Spring leaf area index (LAI), summer photosynthetically active radiation, and autumn and winter air temperatures (T-a) were the most significant controls of monthly ET. Moreover, LAI regulated ET during the whole growing season and higher temperatures increased ET even during dry periods. Our empirical modelling showed that the interaction of LAI and PET explained >90% of the variability in measured ET. Altogether, we found that increases in T-a and shifts in P distribution are likely to impact forest hydrology by altering shallow groundwater fluctuations, soil water storage, and ET and, consequently, alter the ecosystem functions of temperate forests. Copyright (C) 2013 John Wiley & Sons, Ltd. C1 [Xie, Jing; Zha, Tianshan] Beijing Forestry Univ, Coll Soil & Water Conservat, Beijing 100083, Peoples R China. [Xie, Jing; Chu, Hou-Sen; John, Ranjeet; Ouyang, Zutao; Chen, Jiquan] Univ Toledo, Dept Environm Sci, Toledo, OH 43606 USA. [Sun, Ge; McNulty, Steven G.] US Forest Serv, Eastern Forest Environm Threat Assessment Ctr, Southern Res Stn, USDA, Raleigh, NC 27606 USA. [Liu, Junguo; Guan, Wenbin] Beijing Forestry Univ, Coll Nat Conservat, Beijing 100083, Peoples R China. [Noormets, Asko] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA. [Li, Haitao] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Beijing 100101, Peoples R China. [Chen, Jiquan] Nanjing Univ Informat Sci & Technol, Sch Appl Meteorol, Int Ctr Ecol Meteorol & Environm IceMe, Nanjing 210044, Jiangsu, Peoples R China. RP Chen, JQ (reprint author), Nanjing Univ Informat Sci & Technol, Sch Appl Meteorol, Int Ctr Ecol Meteorol & Environm IceMe, Nanjing 210044, Jiangsu, Peoples R China. EM jiquan.chen@.utoledo.edu RI John, Ranjeet/J-2082-2015; Liu, Junguo/B-3021-2012; Chu, Housen/Q-6517-2016; Chen, Jiquan/D-1955-2009; OI John, Ranjeet/0000-0002-0150-8450; Liu, Junguo/0000-0002-5745-6311; Chu, Housen/0000-0002-8131-4938; Noormets, Asko/0000-0003-2221-2111 FU NUIST; Eastern Forest Environmental Threat Assessment Center; Southern Research Station; USDA Forest Service [03-CA-11330147-073, 04-CA-11330147-238]; International Science and Technology Cooperation Program of China [2012DFA91530]; National Natural Science Foundation of China [91025009, 41161140353]; China Scholarship Council [20093012]; Toledo Area Metroparks; US-China Carbon Consortium (USCCC) FX This study was funded by the NUIST, the Eastern Forest Environmental Threat Assessment Center, Southern Research Station, USDA Forest Service (Cooperative Agreements 03-CA-11330147-073 and 04-CA-11330147-238), the International Science and Technology Cooperation Program of China (2012DFA91530), the National Natural Science Foundation of China (91025009 and 41161140353), the China Scholarship Council (20093012), the Toledo Area Metroparks, and the US-China Carbon Consortium (USCCC, which promotes collaborative research among interested institutions in the US and China). We also thank the members of the Landscape Ecology and Ecosystem Sciences Lab at the University of Toledo who provided thoughtful comments, language help, and writing assistance. NR 77 TC 5 Z9 5 U1 3 U2 49 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD DEC 15 PY 2014 VL 28 IS 25 BP 6054 EP 6066 DI 10.1002/hyp.10079 PG 13 WC Water Resources SC Water Resources GA AW5TT UT WOS:000346337400005 ER PT J AU Schafer, KVR Renninger, HJ Clark, KL Medvigy, D AF Schaefer, K. V. R. Renninger, H. J. Clark, K. L. Medvigy, D. TI Hydrological responses to defoliation and drought of an upland oak/pine forest SO HYDROLOGICAL PROCESSES LA English DT Article DE defoliation; drought; sap-flux; upland oak/pine forest; Atlantic Coastal Plain ID CLIMATE-CHANGE; WATER-BALANCE; SAP-FLOW; STOMATAL CONDUCTANCE; CANOPY CONDUCTANCE; NEW-JERSEY; HYDRAULIC ARCHITECTURE; INVASIVE INSECTS; BARK-BEETLE; PINUS-TAEDA AB Hydrologic variability during 2005-2011 was observed and analyzed at an upland oak/pine forest in the New Jersey Pinelands. The forest experienced defoliation by Gypsy moth (Lymantria dispar L.) in 2007, drought conditions in 2006 and a more severe drought in 2010. By using sap flux and eddy covariance measurements, stream discharge data from USGS, soil water changes, precipitation (P) and precipitation throughfall, a local water balance was derived. Average annual canopy transpiration (EC) during 2005-2011 was 201 mm a(-1) +/- 47 mm a(-1). A defoliation event reduced EC by 20% in 2007 compared with the 2005-2011 mean. During drought years in 2006 and 2010, stand transpiration was reduced by 8% in July 2006 and by 18% in 2010, respectively, compared with the overall July average. During July 2007, after the defoliation and subsequent reflushing of half of the leaves, E-C was reduced by 25%. This stand may experience higher sensitivity to drought when recovering from a defoliation event as evidenced by the higher reduction of EC in 2010 (post-defoliation) compared with 2006 (pre-defoliation). Stream water discharge was normalized to the watershed area by dividing outflow with the watershed area. It showed the greatest correlation with transpiration for time lags of 24 days and 219 days, suggesting hydrological connectivity on the watershed scale; stream water discharge increases when transpiration decreases, coinciding with leaf-on and leaf-off conditions. Thus, any changes in transpiration or precipitation will also alter stream water discharge and therefore water availability. Under future climate change, frequency and intensity of precipitation and episodic defoliation events may alter local water balance components in this upland oak/pine forest. Copyright (C) 2013 John Wiley & Sons, Ltd. C1 [Schaefer, K. V. R.; Renninger, H. J.] Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA. [Clark, K. L.] US Forest Serv, USDA, New Lisbon, NJ 08064 USA. [Medvigy, D.] Princeton Univ, Dept Geosci, Princeton, NJ 08544 USA. RP Schafer, KVR (reprint author), Rutgers State Univ, Dept Biol Sci, 195 Univ Ave, Newark, NJ 07102 USA. EM karinavr@andromeda.rutgers.edu RI Renninger, Heidi/E-5237-2015 OI Renninger, Heidi/0000-0002-2485-9835 FU USDA [10-JV-11242306-136]; Office of Science (BER) US Department of Energy [DE-SC0007041] FX USDA joint venture agreement 10-JV-11242306-136 and the Office of Science (BER) US Department of Energy grant DE-SC0007041 supported this research, both to KVRS. NR 70 TC 8 Z9 8 U1 7 U2 40 PU WILEY-BLACKWELL PI HOBOKEN PA 111 RIVER ST, HOBOKEN 07030-5774, NJ USA SN 0885-6087 EI 1099-1085 J9 HYDROL PROCESS JI Hydrol. Process. PD DEC 15 PY 2014 VL 28 IS 25 BP 6113 EP 6123 DI 10.1002/hyp.10104 PG 11 WC Water Resources SC Water Resources GA AW5TT UT WOS:000346337400009 ER PT J AU Lim, GN Maligaspe, E Zandler, ME D'Souza, F AF Lim, Gary N. Maligaspe, Eranda Zandler, Melvin E. D'Souza, Francis TI A Supramolecular Tetrad Featuring Covalently Linked Ferrocene-Zinc Porphyrin-BODIPY Coordinated to Fullerene: A Charge Stabilizing, Photosynthetic Antenna-Reaction Center Mimic SO CHEMISTRY-A EUROPEAN JOURNAL LA English DT Article DE artificial photosynthesis; BODIPY; charge stabilization; ferrocene; fullerenes; electron transfer; zinc porphyrin ID PHOTOINDUCED ELECTRON-TRANSFER; SOLAR-ENERGY CONVERSION; BORON-DIPYRRIN; ARTIFICIAL PHOTOSYNTHESIS; MOLECULAR ASSEMBLIES; AXIAL COORDINATION; ACCEPTOR MOLECULES; SEQUENTIAL ENERGY; SEPARATED STATES; SYSTEMS AB A novel photosynthetic-antenna-reaction-center model compound, comprised of BF2-chelated dipyrromethene (BODIPY) as an energy-harvesting antenna, zinc porphyrin (ZnP) as the primary electron donor, ferrocene (Fc) as a hole-shifting agent, and phenylimidazole-functionalized fulleropyrrolidine (C(60)Im) as an electron acceptor, has been synthesized and characterized. Optical absorption and emission, computational structure optimization, and cyclic voltammetry studies were systematically performed to establish the role of each entity in the multistep photochemical reactions. The energy-level diagram established from optical and redox data helped identifying different photochemical events. Selective excitation of BODIPY resulted in efficient singlet energy transfer to the ZnP entity. Ultrafast electron transfer from the (ZnP)-Zn-1* (formed either as a result of singletsinglet energy transfer or direct excitation) or C-1(60)* of the coordinated fullerene resulting into the formation of the Fc-(C-60(-) Im: ZnPC+)-BODIPY radical ion pair was witnessed by femtosecond transient absorption studies. Subsequent hole migration to the ferrocene entity resulted in the Fc(+)-(C-60(+) Im: ZnP)-BODIPY radical ion pair that persisted for 7-15 ms, depending upon the solvent conditions and contributions from the triplet excited states of ZnP and ImC(60), as revealed by the nanosecond transient spectral studies. Better utilization of light energy in generating the long-lived charge-separated state with the help of the present "antenna- reaction-center" model system has been successfully demonstrated. C1 [Lim, Gary N.; D'Souza, Francis] Univ N Texas, Dept Chem, Denton, TX 76203 USA. [Maligaspe, Eranda; Zandler, Melvin E.] Wichita State Univ, Dept Chem, Wichita, KS 67260 USA. [Maligaspe, Eranda] US Forest Serv, US Forest Prod Lab, USDA, Madison, WI 53726 USA. [Maligaspe, Eranda] Univ Wisconsin, Madison, WI 53726 USA. RP D'Souza, F (reprint author), Univ N Texas, Dept Chem, 1155 Union Circle,305070, Denton, TX 76203 USA. EM Francis.DSouza@UNT.edu FU National Science Foundation [1110942, 1401188] FX This work was supported by National Science Foundation (Grant Nos. 1110942 and 1401188 to FD). NR 101 TC 23 Z9 23 U1 5 U2 73 PU WILEY-V C H VERLAG GMBH PI WEINHEIM PA BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY SN 0947-6539 EI 1521-3765 J9 CHEM-EUR J JI Chem.-Eur. J. PD DEC 15 PY 2014 VL 20 IS 51 BP 17089 EP 17099 DI 10.1002/chem.201404671 PG 11 WC Chemistry, Multidisciplinary SC Chemistry GA AW1NR UT WOS:000346056600032 PM 25339606 ER PT J AU Holzbauer, SM Agger, WA Hall, RL Johnson, GM Schmitt, D Garvey, A Bishop, HS Rivera, H de Almeida, ME Hill, D Stromberg, BE Lynfield, R Smith, KE AF Holzbauer, Stacy M. Agger, William A. Hall, Rebecca L. Johnson, Gary M. Schmitt, David Garvey, Ann Bishop, Henry S. Rivera, Hilda de Almeida, Marcos E. Hill, Dolores Stromberg, Bert E. Lynfield, Ruth Smith, Kirk E. TI Outbreak of Trichinella spiralis Infections Associated With a Wild Boar Hunted at a Game Farm in Iowa SO CLINICAL INFECTIOUS DISEASES LA English DT Article DE human trichinellosis; outbreak; Trichinella spiralis; pork; wild boar ID HUMAN TRICHINOSIS; LARVAE; BRITOVI; HUMANS; MEAT AB Background. Rates of trichinellosis have declined significantly in the United States due to improved pork production practices and public awareness of the danger of eating raw or undercooked pork. In April 2011, the Minnesota Department of Health received a report of presumptive trichinellosis in a 50-year-old man with a history of wild boar consumption. A public health investigation was initiated. Methods. Medical record reviews and patient and family interviews were conducted. Trichinella species serology was performed on patient and family serum samples, and larval identification was attempted on clinical specimens and meat samples. Results. The index patient harvested a wild boar from an Iowa game farm; he processed the meat after returning home and developed gastrointestinal symptoms 2 days later. Four days after his illness onset, all 5 family members consumed a roast from the boar. The index patient sought healthcare 4 times after illness onset before being definitively diagnosed with trichinellosis. Following initiation of albendazole therapy, the index patient developed atrial fibrillation. One additional family member who processed the raw meat was diagnosed with trichinellosis. Trichinella spiralis larvae were identified in wild boar meat samples. Conclusions. Trichinellosis has long been recognized as a potential hazard of consuming undercooked wild carnivore meat, and historically has been associated with consumption of pork from domestic swine, but may be unfamiliar to practicing clinicians in the United States. Education of hunters and the broader population on the potential for trichinellosis and the importance of proper handling and cooking meat from wild or free-range animals needs to be reinforced. C1 [Holzbauer, Stacy M.] Ctr Dis Control & Prevent, Div State & Local Readiness, Off Publ Hlth Preparedness & Response, Atlanta, GA USA. [Holzbauer, Stacy M.; Lynfield, Ruth; Smith, Kirk E.] Minnesota Dept Hlth, St Paul, MN USA. [Agger, William A.] Gunderson Med Fdn, La Crosse, WI USA. [Hall, Rebecca L.; Bishop, Henry S.; Rivera, Hilda; de Almeida, Marcos E.] Ctr Dis Control & Prevent, Ctr Global Hlth, Div Parasit Dis & Malaria, Atlanta, GA USA. [Johnson, Gary M.; Schmitt, David] Iowa Dept Agr & Land Stewardship, Des Moines, IA USA. [Garvey, Ann] Iowa Dept Publ Hlth, Des Moines, IA 50319 USA. [Hill, Dolores] ARS, USDA, Beltsville, MD USA. [Stromberg, Bert E.] Univ Minnesota, Coll Vet Med, Dept Vet & Biomed Sci, St Paul, MN 55108 USA. RP Holzbauer, SM (reprint author), 625 Robert St N, St Paul, MN 55164 USA. EM stacy.holzbauer@state.mn.us NR 31 TC 5 Z9 5 U1 3 U2 23 PU OXFORD UNIV PRESS INC PI CARY PA JOURNALS DEPT, 2001 EVANS RD, CARY, NC 27513 USA SN 1058-4838 EI 1537-6591 J9 CLIN INFECT DIS JI Clin. Infect. Dis. PD DEC 15 PY 2014 VL 59 IS 12 BP 1750 EP 1756 DI 10.1093/cid/ciu713 PG 7 WC Immunology; Infectious Diseases; Microbiology SC Immunology; Infectious Diseases; Microbiology GA AU9KM UT WOS:000345911500020 PM 25214511 ER PT J AU McDonnell, TC Sullivan, TJ Hessburg, PF Reynolds, KM Povak, NA Cosby, BJ Jackson, W Salter, B AF McDonnell, Todd C. Sullivan, Timothy J. Hessburg, Paul F. Reynolds, Keith M. Povak, Nicholas A. Cosby, Bernard J. Jackson, William Salter, Brion TI Steady-state sulfur critical loads and exceedances for protection of aquatic ecosystems in the US southern Appalachian Mountains SO JOURNAL OF ENVIRONMENTAL MANAGEMENT LA English DT Article DE Acidification; Sulfur; Southern Appalachian Mountains; Critical load; Stream ID SURFACE-WATER ACIDIFICATION; MODELING STREAM ACIDIFICATION; CONTERMINOUS UNITED-STATES; CALCULATE CRITICAL LOADS; ACID-BASE CHEMISTRY; FOREST SOILS; AFFORESTED CATCHMENTS; REGIONAL MODEL; MAGIC MODEL; DEPOSITION AB Atmospherically deposited sulfur (S) causes stream water acidification throughout the eastern U.S. Southern Appalachian Mountain (SAM) region. Acidification has been linked with reduced fitness and richness of aquatic species and changes to benthic communities. Maintaining acid-base chemistry that supports native biota depends largely on balancing acidic deposition with the natural resupply of base cations. Stream water acid neutralizing capacity (ANC) is maintained by base cations that mostly originate from weathering of surrounding lithologies. When ambient atmospheric S deposition exceeds the critical load (CL) an ecosystem can tolerate, stream water chemistry may become lethal to biota. This work links statistical predictions of ANC and base cation weathering for streams and watersheds of the SAM region with a steady-state model to estimate CLs and exceedances. Results showed that 20.1% of the total length of study region streams displayed ANC <100 mu eq.L-1, a level at which effects to biota may be anticipated; most were 4th or lower order streams. Nearly one-third of the stream length within the study region exhibited CLs of S deposition <50 meq.m(-2).yr(-1), which is less than the regional average S deposition of 60 meq.m(-2).yr(-1). Owing to their geologic substrates, relatively high elevation, and cool and moist forested conditions, the percentage of stream length in exceedance was highest for mountain wilderness areas and in national parks, and lowest for privately owned valley bottom land. Exceedance results were summarized by 12-digit hydrologic unit code (subwatershed) for use in developing management goals and policy objectives, and for long-term monitoring. (C) 2014 Elsevier Ltd. All rights reserved. C1 [McDonnell, Todd C.; Sullivan, Timothy J.] E&S Environm Chem Inc, Corvallis, OR 97339 USA. [Hessburg, Paul F.; Povak, Nicholas A.; Salter, Brion] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Wenatchee, WA 98801 USA. [Reynolds, Keith M.] US Forest Serv, USDA, Pacific NW Res Stn, Forestry Sci Lab, Corvallis, OR 97331 USA. [Cosby, Bernard J.] Univ Virginia, Dept Environm Sci, Charlottesville, VA 22904 USA. [Jackson, William] US Forest Serv, USDA, Asheville, NC 28801 USA. RP McDonnell, TC (reprint author), E&S Environm Chem Inc, POB 609, Corvallis, OR 97339 USA. EM todd.mcdonnell@esenvironmental.com; tim.sullivan@esenvironmental.com; phessburg@fs.fed.us; kreynolds@fs.fed.us; npovak@fs.fed.us; B.J.Cosby@virginia.edu; bjackson02@fs.fed.us; bsalter@fs.fed.us RI Cosby, Bernard/B-5653-2012; OI Reynolds, Keith/0000-0002-5286-4754 FU U.S. Environmental Protection Agency, Ecosystem Management Decision Support (EMDS); U.S. Forest Service for the southeastern United States [DW-12-92259191-0]; E&S Environmental Chemistry, Inc [AG-3187-C-09-0029] FX This research was funded by the U.S. Environmental Protection Agency, as part of an Ecosystem Management Decision Support (EMDS) modeling pilot study by the U.S. Forest Service for the southeastern United States under interagency agreement DW-12-92259191-0 and a subcontract agreement with E&S Environmental Chemistry, Inc. (Contract AG-3187-C-09-0029). NR 52 TC 2 Z9 2 U1 0 U2 33 PU ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD PI LONDON PA 24-28 OVAL RD, LONDON NW1 7DX, ENGLAND SN 0301-4797 EI 1095-8630 J9 J ENVIRON MANAGE JI J. Environ. Manage. PD DEC 15 PY 2014 VL 146 BP 407 EP 419 DI 10.1016/j.jenvman.2014.07.019 PG 13 WC Environmental Sciences SC Environmental Sciences & Ecology GA AR5HA UT WOS:000343614400044 PM 25135004 ER PT J AU Avila, FW Yang, Y Faquin, V Ramos, SJ Guilherme, LRG Thannhauser, TW Li, L AF Avila, Fabricio William Yang, Yong Faquin, Valdemar Ramos, Silvio Junio Guilherme, Luiz Roberto G. Thannhauser, Theodore W. Li, Li TI Impact of selenium supply on Se-methylselenocysteine and glucosinolate accumulation in selenium-biofortified Brassica sprouts SO FOOD CHEMISTRY LA English DT Article DE Brassica sprouts; Selenium; Se-methylselenocysteine; Glucosinolates; Function food ID PROSTATE-CANCER; BREAST-CANCER; OLERACEA; BROCCOLI; GLUCORAPHANIN; PLANTS; IDENTIFICATION; FERTILIZATION; PERFORMANCE; METABOLISM AB Brassica sprouts are widely marketed as functional foods. Here we examined the effects of Se treatment on the accumulation of anticancer compound Se-methylselenocysteine (SeMSCys) and glucosinolates in Brassica sprouts. Cultivars from the six most extensively consumed Brassica vegetables (broccoli, cauliflower, green cabbage, Chinese cabbage, kale, and Brussels sprouts) were used. We found that Se-biofortified Brassica sprouts all were able to synthesize significant amounts of SeMSCys. Analysis of glucosinolate profiles revealed that each Brassica crop accumulated different types and amounts of glucosinolates. Cauliflower sprouts had high total glucosinolate content. Broccoli sprouts contained high levels of glucoraphanin, a precursor for potent anticancer compound. Although studies have reported an inverse relationship between accumulation of Se and glucosinolates in mature Brassica plants, Se supply generally did not affect glucosinolate accumulation in Brassica sprouts. Thus, Brassica vegetable sprouts can be biofortified with Se for the accumulation of SeMSCys without negative effects on chemopreventive glucosinolate contents. Published by Elsevier Ltd. C1 [Avila, Fabricio William; Yang, Yong; Thannhauser, Theodore W.; Li, Li] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Avila, Fabricio William; Faquin, Valdemar; Guilherme, Luiz Roberto G.] Univ Fed Lavras, Dept Ciencia Solo, BR-37200000 Lavras, MG, Brazil. [Ramos, Silvio Junio] Inst Tecnol Vale, BR-30140130 Belo Horizonte, MG, Brazil. [Avila, Fabricio William; Li, Li] Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA. RP Li, L (reprint author), Cornell Univ, Dept Plant Breeding & Genet, Robert W Holley Ctr Agr & Hlth, USDA ARS, Ithaca, NY 14853 USA. EM ll37@cornell.edu RI Guilherme, Luiz Roberto/B-3998-2009; Avila, Fabricio/B-4910-2014 OI Guilherme, Luiz Roberto/0000-0002-5387-6028; Avila, Fabricio/0000-0003-0301-2720 FU CNPq from Brazil; CAPES from Brazil; FAPEMIG from Brazil; USDA-ARS base fund FX F.W.A. is grateful to the CNPq, CAPES and FAPEMIG, all from Brazil, for financial support and scholarships. This work was supported by the USDA-ARS base fund. USDA is an equal opportunity provider and employer. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. NR 32 TC 9 Z9 11 U1 4 U2 159 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0308-8146 EI 1873-7072 J9 FOOD CHEM JI Food Chem. PD DEC 15 PY 2014 VL 165 BP 578 EP 586 DI 10.1016/j.foodchem.2014.05.134 PG 9 WC Chemistry, Applied; Food Science & Technology; Nutrition & Dietetics SC Chemistry; Food Science & Technology; Nutrition & Dietetics GA AN0WB UT WOS:000340303600075 PM 25038715 ER PT J AU Ladinig, A Gerner, W Saalmuller, A Lunney, JK Ashley, C Harding, JCS AF Ladinig, Andrea Gerner, Wilhelm Saalmueller, Armin Lunney, Joan K. Ashley, Carolyn Harding, John C. S. TI Changes in leukocyte subsets of pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome SO VETERINARY RESEARCH LA English DT Article ID T-CELL SUBSETS; ALVEOLAR MACROPHAGES; PERIPHERAL-BLOOD; IMMUNE-RESPONSES; SWINE-FEVER; PIGS; APOPTOSIS; IDENTIFICATION; PATHOGENESIS; LYMPHOCYTES AB In spite of more than two decades of extensive research, the understanding of porcine reproductive and respiratory syndrome virus (PRRSv) immunity is still incomplete. A PRRSv infection of the late term pregnant female can result in abortions, early farrowings, fetal death, and the birth of weak, congenitally infected piglets. The objectives of the present study were to investigate changes in peripheral blood mononuclear cell populations in third trimester pregnant females infected with type 2 PRRSv (NVSL 97-7895) and to analyze potential relationships with viral load and fetal mortality rate. PRRSv infection caused a massive, acute drop in total leukocyte counts affecting all PBMC populations by two days post infection. Except for B cells, cell counts started to rebound by day six post infection. Our data also show a greater decrease of naive B cells, T-helper cells and cytolytic T cells than their respective effector or memory counterparts. Absolute numbers of T cells and gamma delta T cells were negatively associated with PRRSv RNA concentration in gilt serum over time. Additionally, absolute numbers of T helper cells may be predictive of fetal mortality rate. The preceding three leukocyte populations may therefore be predictive of PRRSv-related pathological outcomes in pregnant gilts. Although many questions regarding the immune responses remain unanswered, these findings provide insight and clues that may help reduce the impact of PRRSv in pregnant gilts. C1 [Ladinig, Andrea; Ashley, Carolyn; Harding, John C. S.] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. [Gerner, Wilhelm; Saalmueller, Armin] Univ Vet Med Vienna, Dept Pathobiol, Inst Immunol, A-1210 Vienna, Austria. [Lunney, Joan K.] ARS, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, USDA, Beltsville, MD USA. RP Ladinig, A (reprint author), Univ Vet Med Vienna, Dept Farm Anim & Vet Publ Hlth, Univ Clin Swine, Vet Pl 1, A-1210 Vienna, Austria. EM andrea.ladinig@vetmeduni.ac.at OI Gerner, Wilhelm/0000-0002-2431-5889; Saalmuller, Armin/0000-0002-7703-3252; Ladinig, Andrea/0000-0001-5037-7269 FU Genome Canada; Genome Prairie [2209-F] FX The authors wish to acknowledge the numerous technicians and students from the Western College of Veterinary Medicine, Vaccine and Infectious Disease Organization, Prairie Diagnostic Services, Inc. and the University of Alberta who assisted with this project. We offer special thanks to Natasa Arsic and Philip Griebel for their greatly valued assistance with the flow cytometry, to Ian Dohoo for his guidance with statistical analyses, and to Graham Plastow for the overall coordination of the project. Pregnant gilts were provided and bred by Fast Genetics Inc., Spiritwood with management support of Dawn Friesen, Connie Heisler, Donell Wingerter and Benny Mote. Funding for the project was generously provided by grants from Genome Canada and Genome Prairie (grant number 2209-F), with administrative support from Genome Alberta. NR 38 TC 4 Z9 4 U1 3 U2 8 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD DEC 14 PY 2014 VL 45 AR 128 DI 10.1186/s13567-014-0128-1 PG 12 WC Veterinary Sciences SC Veterinary Sciences GA AY3MY UT WOS:000347488900001 PM 25497114 ER PT J AU Thame, MM Hsu, JW Gibson, R Baker, TM Tang, GJ Badaloo, AV Fletcher, HM Jackson, AA Jahoor, F AF Thame, Minerva M. Hsu, Jean W. Gibson, Raquel Baker, Tameka M. Tang, Grace J. Badaloo, Asha V. Fletcher, Horace M. Jackson, Alan A. Jahoor, Farook TI Adaptation of in vivo amino acid kinetics facilitates increased amino acid availability for fetal growth in adolescent and adult pregnancies alike SO BRITISH JOURNAL OF NUTRITION LA English DT Article DE Adolescent girls; Adult women; Pregnancies; Protein turnover; Alanine flux; Glutamine flux; Urea flux ID GESTATIONAL-AGE; SOWS INCREASES; BODY-MASS; WOMEN; REQUIREMENT; METABOLISM; OUTCOMES; GLUCOSE; PROTEIN; BIRTH AB During pregnancy, adult women with a normal BMI synthesise extra amino acids after an overnight fast by increasing body protein breakdown and decreasing amino acid oxidation. It is not known whether adolescent girls can make these adaptations during pregnancy. The present study aimed to measure and compare the protein, glutamine and alanine kinetics of adult women and adolescent girls at early-, mid-and late-pregnancy. Kinetics were measured in the overnight fasted state using intravenous infusions of C-13-leucine, N-15-glutamine and N-15-alanine in ten adults and twenty adolescents aged 14-17 years in the first and second trimesters (phase 1 study) and infusions of C-13-leucine and N-15(2)-urea in ten adults and eleven adolescents aged 16-17 years in the first and third trimesters (phase 2 study). In phase 1 study, there were no significant differences between the groups with regard to any of the kinetic parameters measured. In both groups, leucine flux increased (P<0.05), the percentage of leucine flux oxidised decreased (P<0.05) and non-oxidative leucine disposal to protein synthesis increased (P<0.05) from the first to the second trimester. In phase2 study, leucine flux was significantly slower (P<0.05) in the adult group than in the adolescent group during both trimesters, and whole-body leucine flux and non-oxidative leucine disposal increased significantly in the adolescent group (P<0.05, respectively) and were higher in the adult group from the first to the third trimester. These results suggest that similar to their adult counterparts after an overnight fast, adolescent girls with a normal BMI provide extra amino acids required for net protein deposition during pregnancy by increasing protein breakdown and decreasing amino acid oxidation. C1 [Thame, Minerva M.; Baker, Tameka M.] Univ W Indies, Dept Child & Adolescent Hlth, Kingston 7, Jamaica. [Hsu, Jean W.; Tang, Grace J.; Jahoor, Farook] Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Paediat, Houston, TX 77030 USA. [Gibson, Raquel; Fletcher, Horace M.] Univ W Indies, Dept Obstet & Gynaecol, Kingston 7, Jamaica. [Badaloo, Asha V.] Univ W Indies, Trop Metab Res Inst, Kingston 7, Jamaica. [Jackson, Alan A.] Southampton Gen Hosp MP 113, NIHR Southampton Biomed Res Ctr, Southampton SO16 6YD, Hants, England. RP Jahoor, F (reprint author), Baylor Coll Med, Childrens Nutr Res Ctr, USDA ARS, Dept Paediat, Houston, TX 77030 USA. EM fjahoor@bcm.edu FU federal funds from the US Department of Agriculture, Agricultural Research Service [58-6250-6001]; International Atomic Energy Agency; NIHR FX The present study was supported with federal funds from the US Department of Agriculture, Agricultural Research Service under Cooperative Agreement Number 58-6250-6001, and funds from the International Atomic Energy Agency and by the NIHR to Southampton Biomedical Research Centre. NR 30 TC 2 Z9 2 U1 1 U2 3 PU CAMBRIDGE UNIV PRESS PI CAMBRIDGE PA EDINBURGH BLDG, SHAFTESBURY RD, CB2 8RU CAMBRIDGE, ENGLAND SN 0007-1145 EI 1475-2662 J9 BRIT J NUTR JI Br. J. Nutr. PD DEC 14 PY 2014 VL 112 IS 11 BP 1779 EP 1786 DI 10.1017/S000711451400292X PG 8 WC Nutrition & Dietetics SC Nutrition & Dietetics GA AX7NH UT WOS:000347102000004 PM 25322974 ER PT J AU Tecle, IY Edwards, JD Menda, N Egesi, C Rabbi, IY Kulakow, P Kawuki, R Jannink, JL Mueller, LA AF Tecle, Isaak Y. Edwards, Jeremy D. Menda, Naama Egesi, Chiedozie Rabbi, Ismail Y. Kulakow, Peter Kawuki, Robert Jannink, Jean-Luc Mueller, Lukas A. TI solGS: a web-based tool for genomic selection SO BMC BIOINFORMATICS LA English DT Article DE Genomic selection; RR-BLUP; Bioinformatics; Database; Web-based tool ID BREEDING POPULATIONS; PREDICTION AB Background: Genomic selection (GS) promises to improve accuracy in estimating breeding values and genetic gain for quantitative traits compared to traditional breeding methods. Its reliance on high-throughput genome-wide markers and statistical complexity, however, is a serious challenge in data management, analysis, and sharing. A bioinformatics infrastructure for data storage and access, and user-friendly web-based tool for analysis and sharing output is needed to make GS more practical for breeders. Results: We have developed a web-based tool, called solGS, for predicting genomic estimated breeding values (GEBVs) of individuals, using a Ridge-Regression Best Linear Unbiased Predictor (RR-BLUP) model. It has an intuitive web-interface for selecting a training population for modeling and estimating genomic estimated breeding values of selection candidates. It estimates phenotypic correlation and heritability of traits and selection indices of individuals. Raw data is stored in a generic database schema, Chado Natural Diversity, co-developed by multiple database groups. Analysis output is graphically visualized and can be interactively explored online or downloaded in text format. An instance of its implementation can be accessed at the NEXTGEN Cassava breeding database, http://cassavabase.org/solgs. Conclusions: solGS enables breeders to store raw data and estimate GEBVs of individuals online, in an intuitive and interactive workflow. It can be adapted to any breeding program. C1 [Tecle, Isaak Y.; Edwards, Jeremy D.; Menda, Naama; Mueller, Lukas A.] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA. [Egesi, Chiedozie] NRCRI, Umudike, Nigeria. [Rabbi, Ismail Y.; Kulakow, Peter] IITA, Ibadan, Oyo State, Nigeria. [Kawuki, Robert] Natl Crops Resources Res Inst, Kampala, Uganda. [Jannink, Jean-Luc] Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. RP Tecle, IY (reprint author), Cornell Univ, Boyce Thompson Inst Plant Res, Tower Rd, Ithaca, NY 14853 USA. EM iyt2@cornell.edu; lam87@cornell.edu FU NEXTGEN Cassava project; UK Department for International Development FX This work was supported by the NEXTGEN Cassava project, through a grant to Cornell University by the Bill & Melinda Gates Foundation and the UK Department for International Development. We thank Hale Ann Tufan, the NEXTGEN Cassava project manager, for making the discussions possible. We thank to all members of the Cassava project who contributed in many ways. We thank Suzy Strickler for proofreading the manuscript. We thank the anonymous reviewers for their constructive suggestions. NR 28 TC 1 Z9 1 U1 2 U2 18 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2105 J9 BMC BIOINFORMATICS JI BMC Bioinformatics PD DEC 14 PY 2014 VL 15 AR 398 DI 10.1186/s12859-014-0398-7 PG 9 WC Biochemical Research Methods; Biotechnology & Applied Microbiology; Mathematical & Computational Biology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Mathematical & Computational Biology GA AW8NK UT WOS:000346518900001 PM 25495537 ER PT J AU Yang, YR Zhang, QF Kong, YG Ying, YQ Kwok, OCH Liang, H Dubey, JP AF Yang, Yurong Zhang, Qiongfang Kong, Yangguang Ying, Yuqing Kwok, Oliver Chun Hung Liang, Hongde Dubey, Jitender Prakash TI Low prevalence of Neospora caninum and Toxoplasma gondii antibodies in dogs in Jilin, Henan and Anhui Provinces of the People's Republic of China SO BMC VETERINARY RESEARCH LA English DT Article DE Neospora caninum; Toxoplasma gondii; Seroepidemiology; Dogs; China; Modified agglutination test; Neospora agglutination test ID PET DOGS; NORTHEASTERN CHINA; AGGLUTINATION-TEST; STRAY DOGS; SEROPREVALENCE; INFECTION; SHENYANG; OOCYSTS; ELISA AB Background: Neospora caninum and Toxoplasma gondii are important pathogens of worldwide distribution. N. caninum is a major cause of abortion in cattle and dogs are main reservoirs because they excrete the environmentally resistant oocysts. Toxoplasmosis is a worldwide zoonosis and dogs are considered as sentinels for this parasite because of their close contact with people and cats; additionally dog meat is also used for human consumption in China. The aim of the present study was to assess the prevalence of N. caninum and T. gondii infection in dogs from China. A total of 425 countryside dog hearts in Jilin, Henan and Anhui provinces of the People's Republic of China were collected from slaughter houses in two batches; the first batch of 96 in October 2013, and the second batch of 329 in April 2014. Serum samples extracted from 96 dog hearts were tested for antibodies to N. caninum and from 425 dog hearts were tested for T. gondii antibodies in the modified agglutination tests (cut-off 1:25 for both), using respective antigens. Results: Antibodies to N. caninum were 6 of 96 (6.25%) of dogs with titers of 1:25 in 2, 1:50 in 3, and 1:100 in 1. All seropositive dogs were more than 1 year old. Antibodies to T. gondii were found in 35 of 425 (8.24%) dogs with titers of 1:25 in 15, 1:50 in 14; and 1:100 in 6. Conclusion: The results of the present study indicated low prevalence of N. caninum and T. gondii antibodies in dogs of China, compared with Europe and America. Identification of the risk factors that underlie these differences may help prevention of neosporosis and toxoplasmosis. This is the first report of N. caninum infection in dogs from China. C1 [Yang, Yurong; Zhang, Qiongfang; Kong, Yangguang; Liang, Hongde] Henan Agr Univ, Coll Anim Sci & Vet Med, Lab Vet Pathol, Zhengzhou 450002, Peoples R China. [Yang, Yurong; Ying, Yuqing; Kwok, Oliver Chun Hung; Dubey, Jitender Prakash] USDA ARS, Beltsville Agr Res Ctr, Anim Parasit Dis Lab, Beltsville, MD 20705 USA. RP Liang, H (reprint author), Henan Agr Univ, Coll Anim Sci & Vet Med, Lab Vet Pathol, Zhengzhou 450002, Peoples R China. EM hdliang12@163.com FU China Scholarship Council FX This research project was financed in part by China Scholarship Council. NR 32 TC 1 Z9 3 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1746-6148 J9 BMC VET RES JI BMC Vet. Res. PD DEC 12 PY 2014 VL 10 AR 295 DI 10.1186/s12917-014-0295-3 PG 6 WC Veterinary Sciences SC Veterinary Sciences GA AY2LN UT WOS:000347421900001 PM 25495616 ER PT J AU Allen, HK Abedon, ST AF Allen, Heather K. Abedon, Stephen T. TI Virus ecology and disturbances: impact of environmental disruption on the viruses of microorganisms SO FRONTIERS IN MICROBIOLOGY LA English DT Editorial Material DE bacteriophages; environmental disturbance; phage ecology; aquatic microbiology; phage therapy; metaviromes; evolution; microarrays C1 [Allen, Heather K.] ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. [Abedon, Stephen T.] Ohio State Univ, Dept Microbiol, Mansfield, OH USA. RP Allen, HK (reprint author), ARS, Natl Anim Dis Ctr, USDA, Ames, IA 50011 USA. EM heather.allen@ars.usda.gov; abedon.1@osu.edu NR 11 TC 0 Z9 0 U1 4 U2 12 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-302X J9 FRONT MICROBIOL JI Front. Microbiol. PD DEC 12 PY 2014 VL 5 AR 700 DI 10.3389/fmicb.2014.00700 PG 2 WC Microbiology SC Microbiology GA AX9SM UT WOS:000347242400001 PM 25566216 ER PT J AU Scully, ED Geib, SM Carlson, JE Tien, M McKenna, D Hoover, K AF Scully, Erin D. Geib, Scott M. Carlson, John E. Tien, Ming McKenna, Duane Hoover, Kelli TI Functional genomics and microbiome profiling of the Asian longhorned beetle (Anoplophora glabripennis) reveal insights into the digestive physiology and nutritional ecology of wood feeding beetles SO BMC GENOMICS LA English DT Article DE Metatranscriptome; Cerambycidae; Fusarium solani; Nutrient-provisioning; Amino acids; Nitrogen recycling; Xylose fermentation ID BACTERIAL COMMUNITY; GUT MICROBIOTA; COLEOPTERA CERAMBYCIDAE; FUNGAL DIVERSITY; LIFE STAGES; IDENTIFICATION; DEGRADATION; SEQUENCES; DATABASE; PROTEIN AB Background: Wood-feeding beetles harbor an ecologically rich and taxonomically diverse assemblage of gut microbes that appear to promote survival in woody tissue, which is devoid of nitrogen and essential nutrients. Nevertheless, the contributions of these apparent symbionts to digestive physiology and nutritional ecology remain uncharacterized in most beetle lineages. Results: Through parallel transcriptome profiling of beetle- and microbial-derived mRNAs, we demonstrate that the midgut microbiome of the Asian longhorned beetle (Anoplophora glabripennis), a member of the beetle family Cerambycidae, is enriched in biosynthetic pathways for the synthesis of essential amino acids, vitamins, and sterols. Consequently, the midgut microbiome of A. glabripennis can provide essential nutrients that the beetle cannot obtain from its woody diet or synthesize itself. The beetle gut microbiota also produce their own suite of transcripts that can enhance lignin degradation, degrade hemicellulose, and ferment xylose and wood sugars. An abundance of cellulases from several glycoside hydrolase families are expressed endogenously by A. glabripennis, as well as transcripts that allow the beetle to convert microbe-synthesized essential amino acids into non-essential amino acids. A. glabripennis and its gut microbes likely collaborate to digest carbohydrates and convert released sugars and amino acid intermediates into essential nutrients otherwise lacking from their woody host plants. Conclusions: The nutritional provisioning capabilities of the A. glabripennis gut microbiome may contribute to the beetles' unusually broad host range. The presence of some of the same microbes in the guts of other Cerambycidae and other wood-feeding beetles suggests that partnerships with microbes may be a facilitator of evolutionary radiations in beetles, as in certain other groups of insects, allowing access to novel food sources through enhanced nutritional provisioning. C1 [Scully, Erin D.] Penn State Univ, Huck Inst Life Sci, Intercoll Program Genet, University Pk, PA 16802 USA. [Geib, Scott M.] USDA ARS, Pacific Basin Agr Res Ctr, Trop Crop & Commod Protect Res Unit, Hilo, HI 96720 USA. [Carlson, John E.] Penn State Univ, Schatz Ctr Tree Mol Genet, Dept Ecosyst Sci & Management, University Pk, PA 16802 USA. [Tien, Ming] Penn State Univ, Dept Biochem & Mol Biol, University Pk, PA 16802 USA. [McKenna, Duane] Univ Memphis, Dept Biol Sci, Memphis, TN 38152 USA. [Hoover, Kelli] Penn State Univ, Dept Entomol, University Pk, PA 16802 USA. [Hoover, Kelli] Penn State Univ, Ctr Chem Ecol, University Pk, PA 16802 USA. RP Hoover, K (reprint author), Penn State Univ, Dept Entomol, 501 ASI Bldg, University Pk, PA 16802 USA. EM kxh25@psu.edu OI Geib, Scott/0000-0002-9511-5139 FU USDA-NRI-CRSEES grant [2008-35504-04464]; USDA-NRI-CREES grant [2009-35302-05286]; Alphawood Foundation; Chicago; Illinois; Pennsylvania State University College of Agricultural Sciences; USDA-AFRI; World Class University grant from the Ministry of Education, Science and Technology of South Korea [R31-2009-000-20025-0] FX Funding for this project was provided by USDA-NRI-CRSEES grant 2008-35504-04464, USDA-NRI-CREES grant 2009-35302-05286, the Alphawood Foundation, Chicago, Illinois, a Seed Grant to Dr. Hoover from the Pennsylvania State University College of Agricultural Sciences, and a Microbial Genomics Fellowship from USDA-AFRI to EDS. Dr. Carlson was partially supported by World Class University Project R31-2009-000-20025-0 grant from the Ministry of Education, Science and Technology of South Korea. Opinions, findings, conclusions or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the USDA. USDA is an equal opportunity provider and employer. NR 81 TC 11 Z9 11 U1 9 U2 90 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD DEC 12 PY 2014 VL 15 AR 1096 DI 10.1186/1471-2164-15-1096 PG 21 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AX0WW UT WOS:000346671500001 PM 25495900 ER PT J AU Xu, X Variyam, JN Zhao, ZX Chaloupka, FJ AF Xu, Xin Variyam, Jayachandran N. Zhao, Zhenxiang Chaloupka, Frank J. TI Relative Food Prices and Obesity in US Metropolitan Areas: 1976-2001 SO PLOS ONE LA English DT Article ID BODY-MASS INDEX; DIETARY ENERGY DENSITY; PSEUDO-PANEL APPROACH; CROSS-SECTIONS; ADULT OBESITY; TIME-SERIES; TECHNOLOGICAL-CHANGE; PHYSICAL-ACTIVITY; WEIGHT OUTCOMES; BEVERAGE TAXES AB This study investigates the impact of food price on obesity, by exploring the co-occurrence of obesity growth with relative food price reduction between 1976 and 2001. Analyses control for female labor participation and metropolitan outlet densities that might affect body weight. Both the first-difference and fixed effects approaches provide consistent evidence suggesting that relative food prices have substantial impacts on obesity and such impacts were more pronounced among the low-educated. These findings imply that relative food price reductions during the time period could plausibly explain about 18% of the increase in obesity among the U.S. adults in metropolitan areas. C1 [Xu, Xin; Zhao, Zhenxiang] Univ Illinois, Inst Hlth Res & Policy, Chicago, IL 60607 USA. [Variyam, Jayachandran N.] USDA, Econ Res Serv, Food Econ Div, Washington, DE USA. [Chaloupka, Frank J.] Univ Illinois, Natl Bur Econ Res, Inst Hlth Res & Policy, Dept Econ, Chicago, IL USA. RP Xu, X (reprint author), Univ Illinois, Inst Hlth Res & Policy, Chicago, IL 60607 USA. EM xxu11@uic.edu FU United States Department of Agriculture-Economic Research Service Cooperative Agreement [58-4000-9-0047] FX Research for this study was supported by United States Department of Agriculture-Economic Research Service Cooperative Agreement No. 58-4000-9-0047. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 69 TC 0 Z9 0 U1 2 U2 8 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 12 PY 2014 VL 9 IS 12 AR e114707 DI 10.1371/journal.pone.0114707 PG 22 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AW6JI UT WOS:000346375400045 PM 25502888 ER PT J AU Mast, JD De Moraes, CM Alborn, HT Lavis, LD Stern, DL AF Mast, Joshua D. De Moraes, Consuelo M. Alborn, Hans T. Lavis, Luke D. Stern, David L. TI Evolved differences in larval social behavior mediated by novel pheromones SO ELIFE LA English DT Article ID DROSOPHILA-MELANOGASTER LARVAE; MALE COURTSHIP BEHAVIOR; SEX-PHEROMONE; CALOGLYPHUS-POLYPHYLLAE; GUSTATORY NEURONS; OLFACTORY NEURONS; SENSORY NEURONS; CELLULAR BASIS; TASTE; AGGRESSION AB Pheromones, chemical signals that convey social information, mediate many insect social behaviors, including navigation and aggregation. Several studies have suggested that behavior during the immature larval stages of Drosophila development is influenced by pheromones, but none of these compounds or the pheromone-receptor neurons that sense them have been identified. Here we report a larval pheromone-signaling pathway. We found that larvae produce two novel long-chain fatty acids that are attractive to other larvae. We identified a single larval chemosensory neuron that detects these molecules. Two members of the pickpocket family of DEG/ENaC channel subunits (ppk23 and ppk29) are required to respond to these pheromones. This pheromone system is evolving quickly, since the larval exudates of D. simulans, the sister species of D. melanogaster, are not attractive to other larvae. Our results define a new pheromone signaling system in Drosophila that shares characteristics with pheromone systems in a wide diversity of insects. C1 [Mast, Joshua D.; Lavis, Luke D.; Stern, David L.] Howard Hughes Med InstU, Ashburn, VA 20147 USA. [De Moraes, Consuelo M.] ETH, Dept Environm Syst Sci, Zurich, Switzerland. [Alborn, Hans T.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL USA. RP Mast, JD (reprint author), Howard Hughes Med InstU, Janelia Res Campus, Ashburn, VA 20147 USA. EM mastj@janelia.hhmi.org OI Lavis, Luke/0000-0002-0789-6343 FU Howard Hughes Medical Institute FX Howard Hughes Medical Institute David L Stern; The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication. NR 78 TC 14 Z9 14 U1 9 U2 48 PU ELIFE SCIENCES PUBLICATIONS LTD PI CAMBRIDGE PA SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND SN 2050-084X J9 ELIFE JI eLife PD DEC 12 PY 2014 VL 3 AR e04205 DI 10.7554/eLife.04205 PG 21 WC Biology SC Life Sciences & Biomedicine - Other Topics GA AW3FJ UT WOS:000346171300002 PM 25497433 ER PT J AU Kim, DK Lillehoj, HS Jang, SI Lee, SH Hong, YH Cheng, HH AF Kim, Duk Kyung Lillehoj, Hyun S. Jang, Seung I. Lee, Sung Hyen Hong, Yeong Ho Cheng, Hans H. TI Transcriptional Profiles of Host-Pathogen Responses to Necrotic Enteritis and Differential Regulation of Immune Genes in Two Inbreed Chicken Lines Showing Disparate Disease Susceptibility SO PLOS ONE LA English DT Article ID CLOSTRIDIUM-PERFRINGENS; BROILER-CHICKENS; EIMERIA-MAXIMA; LYMPHOID-TISSUES; RISK-FACTORS; ANNEXIN A1; EXPRESSION; RESISTANCE; TENELLA; CELLS AB Necrotic enteritis (NE) is an important intestinal infectious disease of commercial poultry flocks caused by Clostridium perfringens. Using an experimental model of NE involving co-infection with C. perfringens and Eimeria maxima, transcriptome profiling and functional genomics approaches were applied to identify the genetic mechanisms that might regulate the host response to this disease. Microarray hybridization identified 1,049 transcripts whose levels were altered (601 increased, 448 decreased) in intestinal lymphocytes from C. perfringens/E. maxima co-infected Ross chickens compared with uninfected controls. Five biological functions, all related to host immunity and inflammation, and 11 pathways were identified from this dataset. To further elucidate the role of host genetics in NE susceptibility, two inbred chicken lines, ADOL line 6 and line 7 which share an identical B-2 major histocompatibility complex haplotype but differ in their susceptibility to virus infection, were compared for clinical symptoms and the expression levels of a panel of immune-related genes during experimental NE. Line 6 chickens were more susceptible to development of experimental NE compared with line 7, as revealed by decreased body weight gain and increased E. maxima oocyst shedding. Of 21 immune-related genes examined, 15 were increased in C. perfringens/E. maxima co-infected line 6 vs. line 7 chickens. These results suggest that immune pathways are activated in response to experimental NE infection and that genetic determinants outside of the chicken B complex influence resistance to this disease. C1 [Kim, Duk Kyung; Lillehoj, Hyun S.; Jang, Seung I.; Lee, Sung Hyen] Agr Res Serv, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. [Kim, Duk Kyung] Hankyong Natl Univ, Anim Genom & Breeding Ctr, Ansong 456749, Gyeonggi, South Korea. [Lee, Sung Hyen] Natl Acad Agr Sci, Rural Dev Adm, Wanju Gun 565851, Jeollabuk Do, South Korea. [Hong, Yeong Ho] Chung Ang Univ, Dept Anim Sci & Technol, Ansong 456756, South Korea. [Cheng, Hans H.] Agr Res Serv, Avian Dis & Oncol Lab, USDA, E Lansing, MI 48823 USA. RP Lillehoj, HS (reprint author), Agr Res Serv, Anim Biosci & Biotechnol Lab, USDA, Beltsville, MD 20705 USA. EM Hyun.Lillehoj@ars.usda.gov FU ARS CRIS; Next-Generation BioGreen 21 Program [PJ008084, PJ008196]; project 'Investigation of the functional activity and the development of functional foods of Allium hookeri' [PJ010490]; Rural Development Administration, Republic of Korea FX This work was supported by ARS CRIS, partially by grants from the Next-Generation BioGreen 21 Program (No. PJ008084 and No. PJ008196) and the project 'Investigation of the functional activity and the development of functional foods of Allium hookeri (PJ010490)', Rural Development Administration, Republic of Korea. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 37 TC 7 Z9 7 U1 2 U2 9 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 11 PY 2014 VL 9 IS 12 AR e114960 DI 10.1371/journal.pone.0114960 PG 20 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX8FY UT WOS:000347146700076 PM 25504150 ER PT J AU Gao, QM Yu, KS Xia, Y Shine, MB Wang, CX Navarre, D Kachroo, A Kachroo, P AF Gao, Qing-ming Yu, Keshun Xia, Ye Shine, M. B. Wang, Caixia Navarre, DuRoy Kachroo, Aardra Kachroo, Pradeep TI Mono- and Digalactosyldiacylglycerol Lipids Function Nonredundantly to Regulate Systemic Acquired Resistance in Plants SO CELL REPORTS LA English DT Article ID GALACTOLIPID SYNTHESIS; ARABIDOPSIS-THALIANA; CUTICLE FORMATION; SYNTHASE GENES; AZELAIC-ACID; IMMUNITY; DGD1; CHLOROPLASTS; MUTANT; PHOTOSYNTHESIS AB The plant galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) have been linked to the anti-inflammatory and cancer benefits of a green leafy vegetable diet in humans due to their ability to regulate the levels of free radicals like nitric oxide (NO). Here, we show that DGDG contributes to plant NO as well as salicylic acid biosynthesis and is required for the induction of systemic acquired resistance (SAR). In contrast, MGDG regulates the biosynthesis of the SAR signals azelaic acid (AzA) and glycerol-3-phosphate (G3P) that function downstream of NO. Interestingly, DGDG is also required for AzA-induced SAR, but MGDG is not. Notably, transgenic expression of a bacterial glucosyltransferase is unable to restore SAR in dgd1 plants even though it does rescue their morphological and fatty acid phenotypes. These results suggest that MGDG and DGDG are required at distinct steps and function exclusively in their individual roles during the induction of SAR. C1 [Gao, Qing-ming; Yu, Keshun; Xia, Ye; Shine, M. B.; Wang, Caixia; Kachroo, Aardra; Kachroo, Pradeep] Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. [Wang, Caixia] Qingdao Agr Univ, Qingdao 266109, Peoples R China. [Navarre, DuRoy] Washington State Univ, USDA ARS, Prosser, WA 99350 USA. RP Kachroo, A (reprint author), Univ Kentucky, Dept Plant Pathol, Lexington, KY 40546 USA. EM apkach2@uky.edu; pk62@uky.edu FU National Science Foundation [0749731, 0421914]; Kentucky Science and Engineering Foundation [2930-RDE-016] FX We thank Georg Holzl and Peter Dormann for dgd1 complemented lines and dgd2 seeds, Henrik Aronsson for mgd1 seeds, John Johnson for help with gas chromatography, Andrew Gifford and Joanna Fowler for 14C-AzA, Joanne Holden for help with SA analysis, and Ludmila Lapchyk for technical help. We thank Christoph Benning for useful advice on galactolipid profiling and Peter Dormann, David Wendehenne, Maelor Davies, Wolf-Dieter Reiter, and Meenakshi Upreti for useful suggestions. This work was supported by grants from National Science Foundation (IOS#0749731, MCB#0421914) and Kentucky Science and Engineering Foundation (2930-RDE-016). NR 25 TC 11 Z9 11 U1 4 U2 26 PU CELL PRESS PI CAMBRIDGE PA 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA SN 2211-1247 J9 CELL REP JI Cell Reports PD DEC 11 PY 2014 VL 9 IS 5 BP 1681 EP 1691 DI 10.1016/j.celrep.2014.10.069 PG 11 WC Cell Biology SC Cell Biology GA AX3PR UT WOS:000346851900014 PM 25466253 ER PT J AU Fuerst, EP Okubara, PA Anderson, JV Morris, CF AF Fuerst, E. Patrick Okubara, Patricia A. Anderson, James V. Morris, Craig F. TI Polyphenol oxidase as a biochemical seed defense mechanism SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE seed defense; seed decay; seed longevity; weed seed bank ID OXALATE OXIDASE; GERMIN GENE; DIFFERENTIAL EXPRESSION; MULTIGENE FAMILY; SIGNAL PEPTIDES; WHEAT; PLANT; PROTEINS; FUNGI; L. AB Seed dormancy and resistance to decay are fundamental survival strategies, which allow a population of seeds to germinate over long periods of time. Seeds have physical, chemical, and biological defense mechanisms that protect their food reserves from decay-inducing organisms and herbivores. Here, we hypothesize that seeds also possess enzyme-based biochemical defenses, based on induction of the plant defense enzyme, polyphenol oxidase (PRO), when wild oat (Avena fatua L.) caryopses and seeds were challenged with seed-decaying Fusarium fungi. These studies suggest that dormant seeds are capable of mounting a defense response to pathogens. The pathogen-induced PRO activity from wild oat was attributed to a soluble isoform of the enzyme that appeared to result, at least in part, from proteolytic activation of a latent PRO isoform. PRO activity was also induced in wild oat hulls (lemma and palea), non-living tissues that cover and protect the caryopsis. These results are consistent with the hypothesis that seeds possess inducible enzyme-based biochemical defenses arrayed on the exterior of seeds and these defenses represent a fundamental mechanism of seed survival and longevity in the soil. Enzyme-based biochemical defenses may have broader implications since they may apply to other defense enzymes as well as to a diversity of plant species and ecosystems. C1 [Fuerst, E. Patrick] Washington State Univ, Dept Crop & Soil Sci, Pullman, WA 99164 USA. [Okubara, Patricia A.] Washington State Univ, USDA ARS, Root Dis & Biol Control Res Unit, Pullman, WA USA. [Anderson, James V.] USDA ARS, Biosci Res Lab, Fargo, ND 58105 USA. [Morris, Craig F.] Washington State Univ, USDA ARS, Western Wheat Qual Lab, Pullman, WA 99164 USA. RP Fuerst, EP (reprint author), Washington State Univ, Dept Crop & Soil Sci, E-202 Food Qual Bldg, Pullman, WA 99164 USA. EM pfuerst@wsu.edu FU Agriculture and Food Research Initiative Grant from the USDA National Institute of Food and Agriculture [2013-02322, 2014-67013-21575]; United States Department of Agriculture, Agricultural Research Service [5248-22000-012-00D] FX This project was supported by the Agriculture and Food Research Initiative Grant Number 2013-02322 (Award Number: 2014-67013-21575) from the USDA National Institute of Food and Agriculture, and by the United States Department of Agriculture, Agricultural Research Service Project Number 5248-22000-012-00D (Patricia A. Okubara). NR 75 TC 4 Z9 4 U1 2 U2 27 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD DEC 10 PY 2014 VL 5 AR 689 DI 10.3389/fpls.2014.00689 PG 9 WC Plant Sciences SC Plant Sciences GA AY7ZK UT WOS:000347774100001 PM 25540647 ER PT J AU Menolli, N Justo, A Arrillaga, P Pradeep, CK Minnis, AM Capelari, M AF Menolli, Nelson, Jr. Justo, Alfredo Arrillaga, Pedro Pradeep, C. K. Minnis, Andrew M. Capelari, Marina TI Taxonomy and phylogeny of Pluteus glaucotinctus sensu lato (Agaricales, Basidiomycota), a multicontinental species complex SO PHYTOTAXA LA English DT Article DE nrITS; Pluteaceae; section Pluteus; species delimitation; tef1 ID PLUTEACEAE AB In order to better understand species delimitation in the Pluteus glaucotinctus species complex, we present a detailed study based on morphological and DNA sequence (nrITS + tef1) data. Pluteus glaucotinctus sensu stricto is known only from the type collection (Democratic Republic of the Congo), which is re-examined here. Four other species are recognized in this complex and described herein as new: P. izurun from Spain, P. meridionalis from Brazil, P. padanilus from India and P. thoenii from Africa. Phylogenetic analyses based on nrITS + tef1 sequence data support the recognition of all species in this complex. C1 [Menolli, Nelson, Jr.] Inst Fed Educ & Ciencia Tecnol Sao Paulo, CCT Biol, BR-01109010 Sao Paulo, Brazil. [Menolli, Nelson, Jr.; Capelari, Marina] Inst Bot, BR-04045972 Sao Paulo, Brazil. [Justo, Alfredo] Clark Univ, Dept Biol, Worcester, MA 01610 USA. [Arrillaga, Pedro] Sociedad Ciencias Aranzadi, Dept Micol, San Sebastian 20014, Spain. [Pradeep, C. K.] Jawaharlal Nehru Trop Bot Garden & Res Inst, Trivandrum 695562, Kerala, India. [Minnis, Andrew M.] USDA, US Forest Serv, Ctr Forest Mycol Res, Madison, WI 53726 USA. RP Menolli, N (reprint author), Inst Fed Educ & Ciencia Tecnol Sao Paulo, CCT Biol, Campus Sao Paulo,Rua Pedro Vicente 625, BR-01109010 Sao Paulo, Brazil. EM menollijr@yahoo.com.br RI Capelari, Marina /D-4886-2015; Menolli Jr, Nelson/N-3478-2013 FU "Fundacao de Amparo a Pesquisa do Estado de Sao Paulo" (FAPESP) [04/04319-2, 09/53272-2]; "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq); National Science Foundation [DEB 0933081]; U.S. Forest Service funds FX The authors thank the curator of BR for managing the loans of the specimens from Africa and Luiz Antonio S. Ramos for assistance with formatting the plates. N. Menolli Jr. and M. Capelari thank Fernanda Karstedt and L. A. S. Ramos for collecting some specimens of P. meridionalis; Francisco Kuhar for the suggestion of the name P. meridionalis, the "Fundacao de Amparo a Pesquisa do Estado de Sao Paulo" (FAPESP grants 04/04319-2, 09/53272-2) and the "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico" (CNPq fellowships) for financial support. A. Justo acknowledges support from the National Science Foundation grant DEB 0933081. A. M. Minnis expresses gratitude for support from Daniel L. Lindner and U.S. Forest Service funds. NR 33 TC 3 Z9 3 U1 1 U2 4 PU MAGNOLIA PRESS PI AUCKLAND PA PO BOX 41383, AUCKLAND, ST LUKES 1030, NEW ZEALAND SN 1179-3155 EI 1179-3163 J9 PHYTOTAXA JI Phytotaxa PD DEC 10 PY 2014 VL 188 IS 2 BP 78 EP 90 PG 13 WC Plant Sciences SC Plant Sciences GA AY4KB UT WOS:000347545600002 ER PT J AU Pandey, MK Wang, ML Qiao, LX Feng, SP Khera, P Wang, H Tonnis, B Barkley, NA Wang, JP Holbrook, CC Culbreath, AK Varshney, RK Guo, BZ AF Pandey, Manish K. Wang, Ming Li Qiao, Lixian Feng, Suping Khera, Pawan Wang, Hui Tonnis, Brandon Barkley, Noelle A. Wang, Jianping Holbrook, C. Corley Culbreath, Albert K. Varshney, Rajeev K. Guo, Baozhu TI Identification of QTLs associated with oil content and mapping FAD2 genes and their relative contribution to oil quality in peanut (Arachis hypogaea L.) SO BMC GENETICS LA English DT Article DE Peanut; Genetic map; QTL analysis; Oil content; Oleic acid; Linoleic acid; O/L ratio; FAD2 genes ID HIGH OLEIC-ACID; HIGH OLEATE TRAIT; CULTIVATED PEANUT; LINKAGE MAP; REGISTRATION; PROFILES; POPULATIONS; RESISTANCE; STABILITY; GENOMICS AB Background: Peanut is one of the major source for human consumption worldwide and its seed contain approximately 50% oil. Improvement of oil content and quality traits (high oleic and low linoleic acid) in peanut could be accelerated by exploiting linked markers through molecular breeding. The objective of this study was to identify QTLs associated with oil content, and estimate relative contribution of FAD2 genes (ahFAD2A and ahFAD2B) to oil quality traits in two recombinant inbred line (RIL) populations. Results: Improved genetic linkage maps were developed for S-population (SunOleic 97R NC94022) with 206 (1780.6 cM) and T-population (Tifrunner GT-C20) with 378 (2487.4 cM) marker loci. A total of 6 and 9 QTLs controlling oil content were identified in the S and T population, respectively. The contribution of each QTL towards oil content variation ranged from 3.07 to 10.23% in the S-population and from 3.93 to 14.07% in the T-population. The mapping positions for ahFAD2A (A sub-genome) and ahFAD2B (B sub-genome) genes were assigned on a09 and b09 linkage groups. The ahFAD2B gene (26.54%, 25.59% and 41.02% PVE) had higher phenotypic effect on oleic acid (C18:1), linoleic acid (C18: 2), and oleic/linoleic acid ratio (O/L ratio) than ahFAD2A gene (8.08%, 6.86% and 3.78% PVE). The FAD2 genes had no effect on oil content. This study identified a total of 78 main-effect QTLs (M-QTLs) with up to 42.33% phenotypic variation (PVE) and 10 epistatic QTLs (E-QTLs) up to 3.31% PVE for oil content and quality traits. Conclusions: A total of 78 main-effect QTLs (M-QTLs) and 10 E-QTLs have been detected for oil content and oil quality traits. One major QTL (more than 10% PVE) was identified in both the populations for oil content with source alleles from NC94022 and GT-C20 parental genotypes. FAD2 genes showed high effect for oleic acid (C18: 1), linoleic acid (C18: 2), and O/L ratio while no effect on total oil content. The information on phenotypic effect of FAD2 genes for oleic acid, linoleic acid and O/L ratio, and oil content will be applied in breeding selection. C1 [Pandey, Manish K.; Qiao, Lixian; Feng, Suping; Khera, Pawan; Wang, Hui; Guo, Baozhu] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [Pandey, Manish K.; Khera, Pawan; Varshney, Rajeev K.] Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. [Pandey, Manish K.; Qiao, Lixian; Feng, Suping; Khera, Pawan; Wang, Hui; Culbreath, Albert K.; Guo, Baozhu] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Wang, Ming Li; Tonnis, Brandon; Barkley, Noelle A.] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA USA. [Qiao, Lixian] Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China. [Feng, Suping] Qiongzhou Univ, Coll Biosci & Biotechnol, Sanya, Peoples R China. [Wang, Hui] Shandong Acad Agr Sci, Peanut Res Inst, Qingdao, Peoples R China. [Wang, Jianping] Univ Florida, Dept Agron, Gainesville, FL 32611 USA. [Holbrook, C. Corley] USDA ARS, Crop Genet & Breeding Res Unit, Tifton, GA 31793 USA. RP Varshney, RK (reprint author), Int Crops Res Inst Semi Arid Trop, Hyderabad, Andhra Pradesh, India. EM r.k.varshney@cgiar.org; baozhu.guo@ars.usda.gov RI Varshney, Rajeev/C-5295-2014 OI Varshney, Rajeev/0000-0002-4562-9131 FU USDA Agricultural Research Service; Georgia Agricultural Commodity Commission for Peanuts; Peanut Foundation; National Peanut Board FX We thank Billy Wilson, Jake Fountain, Stephanie Lee, Lucero Gutierrez and Sara Beth Pelham for technical assistance in the field and the laboratory work. A special appreciation will be to David Pinnow for helping in oil content measurement. This research was partially supported by funds provided by the USDA Agricultural Research Service, the Georgia Agricultural Commodity Commission for Peanuts, Peanut Foundation and National Peanut Board. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This work has been undertaken as part of the CGIAR Research Program on Grain Legumes. ICRISAT is a member of CGIAR Consortium. NR 43 TC 14 Z9 15 U1 3 U2 21 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD DEC 10 PY 2014 VL 15 AR 133 DI 10.1186/s12863-014-0133-4 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA AX9EY UT WOS:000347207700001 PM 25491595 ER PT J AU Temeyer, KB Tong, F Totrov, MM Tuckow, AP Chen, QH Carlier, PR de Leon, AAP Bloomquist, JR AF Temeyer, Kevin B. Tong, Fan Totrov, Maxim M. Tuckow, Alexander P. Chen, Qiao-hong Carlier, Paul R. de Leon, Adalberto A. Perez Bloomquist, Jeffrey R. TI Acetylcholinesterase of the sand fly, Phlebotomus papatasi (Scopoli): construction, expression and biochemical properties of the G119S orthologous mutant SO PARASITES & VECTORS LA English DT Article DE Sand fly; Acetylcholinesterase inhibition; P. papatasi; cDNA; AChE ID ZOONOTIC CUTANEOUS LEISHMANIASIS; CULEX-PIPIENS MOSQUITOS; TALLIL AIR BASE; INSECTICIDE RESISTANCE; ANOPHELES-GAMBIAE; INSENSITIVE ACETYLCHOLINESTERASE; MILITARY OPERATIONS; LIGAND DOCKING; RESERVOIR HOST; FLIES AB Background: Phlebotomus papatasi vectors zoonotic cutaneous leishmaniasis. Previous expression of recombinant P. papatasi acetylcholinesterase (PpAChE1) revealed 85% amino acid sequence identity to mosquito AChE and identified synthetic carbamates that effectively inhibited PpAChE1 with improved specificity for arthropod AChEs compared to mammalian AChEs. We hypothesized that the G119S mutation causing high level resistance to organophosphate insecticides in mosquitoes may occur in PpAChE1 and may reduce sensitivity to inhibition. We report construction, expression, and biochemical properties of rPpAChE1 containing the G119S orthologous mutation. Methods: Targeted mutagenesis introduced the G119S orthologous substitution in PpAChE1 cDNA. Recombinant PpAChE1 enzymes containing or lacking the G119S mutation were expressed in the baculoviral system. Biochemical assays were conducted to determine altered catalytic properties and inhibitor sensitivity resulting from the G119S substitution. A molecular homology model was constructed to examine the modeled structural interference with docking of inhibitors of different classes. Genetic tests were conducted to determine if the G119S orthologous codon existed in polymorphic form in a laboratory colony of P. papatasi. Results: Recombinant PpAChE1 containing the G119S substitution exhibited altered biochemical properties, and reduced inhibition by compounds that bind to the acylation site on the enzyme (with the exception of eserine). Less resistance was directed against bivalent or peripheral site inhibitors, in good agreement with modeled inhibitor docking. Eserine appeared to be a special case capable of inhibition in the absence of covalent binding at the acylation site. Genetic tests did not detect the G119S mutation in a laboratory colony of P. papatasi but did reveal that the G119S codon existed in polymorphic form (GGA + GGC). Conclusions: The finding of G119S codon polymorphism in a laboratory colony of P. papatasi suggests that a single nucleotide transversion (GGC -> AGC) may readily occur, causing rapid development of resistance to organophosphate and phenyl- substituted carbamate insecticides under strong selection. Careful management of pesticide use in IPM programs is important to prevent or mitigate development and fixation of the G119S mutation in susceptible pest populations. Availability of recombinant AChEs enables identification of novel inhibitory ligands with improved efficacy and specificity for AChEs of arthropod pests. C1 [Temeyer, Kevin B.; Tuckow, Alexander P.; de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX 78028 USA. [Tong, Fan; Bloomquist, Jeffrey R.] Univ Florida, Dept Entomol & Nematol, Emerging Pathogens Inst, Gainesville, FL 32610 USA. [Totrov, Maxim M.] Molsoft LLC, La Jolla, CA 92037 USA. [Chen, Qiao-hong; Carlier, Paul R.] Virginia Tech, Dept Chem, Blacksburg, VA 24061 USA. RP Temeyer, KB (reprint author), USDA ARS, Knipling Bushland US Livestock Insects Res Lab, 2700 Fredericksburg Rd, Kerrville, TX 78028 USA. EM kevin.temeyer@ars.usda.gov FU U.S. Department of Agriculture [USDA] (USDA-ARS CRIS) [6205-32000-033000D]; USDA-ARS CRADA [60-0208-9-601, 6201-32000-033017R]; Deployed Warfighter Protection Research Program [DWFP] of the U.S. Department of Defense through the Armed Forces Pest Management Board [AFPMB]; USDA [108-0128-098]; AFPMB FX The authors thank Kristie Schlechte for excellent technical assistance. This work was funded by U.S. Department of Agriculture [USDA] appropriated funds (USDA-ARS CRIS number 6205-32000-033000D, Molecular biology and physiology of biting flies affecting livestock) and USDA-ARS CRADA number 60-0208-9-601 (Project number 6201-32000-033017R, Deployed Warfighter Protection Research Program [DWFP] of the U.S. Department of Defense through the Armed Forces Pest Management Board [AFPMB]). Additional funding was provided by USDA Specific Cooperative Agreement 108-0128-098 to JRB as part of the DWFP and the AFPMB. NR 53 TC 3 Z9 3 U1 1 U2 10 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1756-3305 J9 PARASITE VECTOR JI Parasites Vectors PD DEC 10 PY 2014 VL 7 AR 577 DI 10.1186/s13071-014-0577-4 PG 10 WC Parasitology SC Parasitology GA AX9ZJ UT WOS:000347257200001 PM 25491113 ER PT J AU Ahmadi, N Audebert, A Bennett, MJ Bishopp, A de Oliveira, AC Courtois, B Diedhiou, A Dievart, A Gantet, P Ghesquiere, A Guiderdoni, E Henry, A Inukai, Y Kochian, L Laplaze, L Lucas, M Luu, DT Manneh, B Mo, XR Muthurajan, R Perin, C Price, A Robin, S Sentenac, H Sine, B Uga, Y Very, AA Wissuwa, M Wu, P Xu, J AF Ahmadi, Nourollah Audebert, Alain Bennett, Malcolm J. Bishopp, Anthony de Oliveira, Antonio Costa Courtois, Brigitte Diedhiou, Abdala Dievart, Anne Gantet, Pascal Ghesquiere, Alain Guiderdoni, Emmanuel Henry, Amelia Inukai, Yoshiaki Kochian, Leon Laplaze, Laurent Lucas, Mikael Doan Trung Luu Manneh, Baboucarr Mo, Xiaorong Muthurajan, Raveendran Perin, Christophe Price, Adam Robin, Sabariappan Sentenac, Herve Sine, Bassirou Uga, Yusaku Very, Anne Alienor Wissuwa, Matthias Wu, Ping Xu, Jian TI The roots of future rice harvests SO RICE LA English DT Review DE Ideotype; Breeding; Phenotyping; Genetic and molecular controls; Rice; Roots ID HIGH-THROUGHPUT ANALYSIS; SYSTEM ARCHITECTURE; SOFTWARE PLATFORM; ORYZA-SATIVA; WATER-UPTAKE; UPLAND RICE; OUTER PART; DROUGHT; GROWTH; YIELD AB Rice production faces the challenge to be enhanced by 50% by year 2030 to meet the growth of the population in rice-eating countries. Whereas yield of cereal crops tend to reach plateaus and a yield is likely to be deeply affected by climate instability and resource scarcity in the coming decades, building rice cultivars harboring root systems that can maintain performance by capturing water and nutrient resources unevenly distributed is a major breeding target. Taking advantage of gathering a community of rice root biologists in a Global Rice Science Partnership workshop held in Montpellier, France, we present here the recent progresses accomplished in this area and focal points where an international network of laboratories should direct their efforts. C1 [Ahmadi, Nourollah; Audebert, Alain; Courtois, Brigitte; Dievart, Anne; Guiderdoni, Emmanuel; Perin, Christophe] CIRAD, UMR AGAP, F-34398 Montpellier 5, France. [Bennett, Malcolm J.; Bishopp, Anthony] Univ Nottingham, Ctr Plant Integrat Biol, Loughborough LE12 5RD, Leics, England. [de Oliveira, Antonio Costa] Univ Fed Pelotas, Plant Genom & Breeding Ctr, Pelotas, Brazil. [Diedhiou, Abdala] UCAD, Ctr Rech Bel Air, Lab Commun Microbiol IRD ISRA UCAD, Dept Biol Vegetale, Dakar 18524, Senegal. [Diedhiou, Abdala; Laplaze, Laurent; Sine, Bassirou] Ctr Rech Bel Air, Lab Mixte Int Adaptat Plantes & Microorganismes A, Dakar 18524, Senegal. [Gantet, Pascal] Univ Montpellier 2, UMR DIADE, Montpellier, France. [Gantet, Pascal] USTH, Agron Genet Inst, IRD, LMI,RICE, Hanoi, Vietnam. [Ghesquiere, Alain; Laplaze, Laurent; Lucas, Mikael] IRD, UMR DIADE, Montpellier, France. [Henry, Amelia] IRRI, Los Banos, Philippines. [Inukai, Yoshiaki] Nagoya Univ, ICCAE, Chikusa Ku, Nagoya, Aichi 4648601, Japan. [Kochian, Leon] USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA. [Kochian, Leon] Cornell Univ, Dept Plant Biol, Ithaca, NY 14853 USA. [Doan Trung Luu; Very, Anne Alienor] Univ Montpellier 2, Inst Biol Integrat Plantes, UMR CNRS 5004, Montpellier SupAgro,INRA 386, F-34060 Montpellier 2, France. [Manneh, Baboucarr] Africa Rice Ctr, AfricaRice Sahel Reg Stn, St Louis, Senegal. [Mo, Xiaorong] Zhejiang Univ, Coll Life Sci, State Key Lab Plant Physiol & Biochem, Hangzhou 310058, Zhejiang, Peoples R China. [Muthurajan, Raveendran; Robin, Sabariappan] Tamil Nadu Agr Univ, Coimbatore 641003, Tamil Nadu, India. [Price, Adam] Univ Aberdeen, Aberdeen AB24 3UU, Scotland. [Sine, Bassirou] ISRA, CERAAS, Thies, Senegal. [Uga, Yusaku] NIAS, Tsukuba, Ibaraki 3058602, Japan. [Wissuwa, Matthias] JIRCAS, Tsukuba, Ibaraki 3058686, Japan. [Xu, Jian] Natl Univ Singapore, Dept Biol Sci, Fac Sci, Singapore 117543, Singapore. [Xu, Jian] Natl Univ Singapore, NUS Ctr BioImaging Sci, Fac Sci, Singapore 117543, Singapore. RP Guiderdoni, E (reprint author), CIRAD, UMR AGAP, F-34398 Montpellier 5, France. EM guiderdoni@cirad.fr RI GHESQUIERE, Alain/K-3068-2016; LUCAS, Mikael/A-5925-2015; Perin, Christophe/B-4992-2008; Laplaze, Laurent/I-5435-2016 OI Kochian, Leon/0000-0003-3416-089X; Oliveira, Antonio/0000-0001-8835-8071; Luu, Doan Trung/0000-0001-9765-2125; Sine, Bassirou/0000-0002-1978-8446; Laplaze, Laurent/0000-0002-6568-6504 FU Global Rice Science Partnership and Agropolis Fondation [1400-009, 1001-005]; ANR "Investissement d'Avenir" grant [ANR-10-LABX-001-01]; Generation Challenge Program; Bill and Melinda Gates Foundation; AcRF Tier 2 grant from the Ministry of Education of Singapore [MOE2009-T2-1-060]; National Research Foundation Singapore under its Competitive Research Programme (CRP Award) [NRF2010NRF-CRP002-018]; EU Marie Curie International Outgoing Fellowship 'ORYZAQUA - Cell Biology of Rice Aquaporins' [PIOF-GA-2011-300150]; Generation Challenge Programme; Universite Cheikh Anta Diop (UCAD) [VE12/13, CpVIII-Ar4]; GRISP; [289300.27 EURoot] FX The authors thank the Global Rice Science Partnership and Agropolis Fondation (Special grant no 1400-009 and Rhizopolis grant no 1001-005) benefiting from a national ANR "Investissement d'Avenir" grant ANR-10-LABX-001-01) for supporting the workshop. They acknowledge the assistance of Nathalie Pivot, Cirad and Veronique Rafin, INRA in workshop organization. The root research at Cirad and University of Aberdeen is supported by the European Grant (FP7/2007-2013) under grant agreement no 289300.27 EURoot "Enhancing resource Uptake from ROOTs under stress in cereal crops". Research at IRRI is supported by the Generation Challenge Program and the Bill and Melinda Gates Foundation. J.X. is supported by the AcRF Tier 2 grant (MOE2009-T2-1-060) from the Ministry of Education of Singapore and National Research Foundation Singapore under its Competitive Research Programme (CRP Award No. NRF2010NRF-CRP002-018). Doan Trung Luu is supported by the EU Marie Curie International Outgoing Fellowship 'ORYZAQUA - Cell Biology of Rice Aquaporins' (PIOF-GA-2011-300150). AP acknowledges the Generation Challenge Programme funded project "Targeting drought avoidance root traits to enhance rice productivity under water limited environments". Financial support for A.G. Diedhiou was provided by the Universite Cheikh Anta Diop (UCAD, VE12/13, CpVIII-Ar4) and GRISP. NR 72 TC 4 Z9 5 U1 8 U2 67 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1939-8425 EI 1939-8433 J9 RICE JI Rice PD DEC 10 PY 2014 VL 7 AR 29 DI 10.1186/s12284-014-0029-y PG 9 WC Agronomy SC Agriculture GA AX5MC UT WOS:000346969500001 PM 26224558 ER PT J AU Vaziri, ND Liu, SM Lau, WL Khazaeli, M Nazertehrani, S Farzaneh, SH Kieffer, DA Adams, SH Martin, RJ AF Vaziri, Nosratola D. Liu, Shu-Man Lau, Wei Ling Khazaeli, Mahyar Nazertehrani, Sohrab Farzaneh, Seyed H. Kieffer, Dorothy A. Adams, Sean H. Martin, Roy J. TI High Amylose Resistant Starch Diet Ameliorates Oxidative Stress, Inflammation, and Progression of Chronic Kidney Disease SO PLOS ONE LA English DT Article ID GUT BACTERIAL TRANSLOCATION; EPITHELIAL TIGHT JUNCTION; IMPAIRS BARRIER FUNCTION; STAGE RENAL-DISEASE; CARBOHYDRATE AVAILABILITY; INTESTINAL MICROBIOTA; INDOLIC COMPOUNDS; UREMIA; MICROINFLAMMATION; MECHANISM AB Inflammation is a major mediator of CKD progression and is partly driven by altered gut microbiome and intestinal barrier disruption, events which are caused by: urea influx in the intestine resulting in dominance of urease-possessing bacteria; disruption of epithelial barrier by urea-derived ammonia leading to endotoxemia and bacterial translocation; and restriction of potassium-rich fruits and vegetables which are common sources of fermentable fiber. Restriction of these foods leads to depletion of bacteria that convert indigestible carbohydrates to short chain fatty acids which are important nutrients for colonocytes and regulatory T lymphocytes. We hypothesized that a high resistant starch diet attenuates CKD progression. Male Sprague Dawley rats were fed a chow containing 0.7% adenine for 2 weeks to induce CKD. Rats were then fed diets supplemented with amylopectin (low-fiber control) or high fermentable fiber (amylose maize resistant starch, HAM-RS2) for 3 weeks. CKD rats consuming low fiber diet exhibited reduced creatinine clearance, interstitial fibrosis, inflammation, tubular damage, activation of NF kappa B, upregulation of pro-inflammatory, pro-oxidant, and pro-fibrotic molecules; impaired Nrf2 activity, down-regulation of antioxidant enzymes, and disruption of colonic epithelial tight junction. The high resistant starch diet significantly attenuated these abnormalities. Thus high resistant starch diet retards CKD progression and attenuates oxidative stress and inflammation in rats. Future studies are needed to explore the impact of HAM-RS2 in CKD patients. C1 [Vaziri, Nosratola D.; Liu, Shu-Man; Lau, Wei Ling; Khazaeli, Mahyar; Nazertehrani, Sohrab; Farzaneh, Seyed H.] Univ Calif Irvine, Div Nephrol, Irvine, CA 92717 USA. [Kieffer, Dorothy A.; Adams, Sean H.; Martin, Roy J.] Univ Calif Davis, Grad Grp Nutr Biol, Sacramento, CA 95817 USA. [Kieffer, Dorothy A.; Adams, Sean H.; Martin, Roy J.] Univ Calif Davis, Dept Nutr, Sacramento, CA 95817 USA. [Kieffer, Dorothy A.; Adams, Sean H.] USDA ARS, Western Human Nutr Res Ctr, Obes & Metab Res Unit, Davis, CA USA. RP Vaziri, ND (reprint author), Univ Calif Irvine, Div Nephrol, Irvine, CA 92717 USA. EM ndvaziri@uci.edu RI Liu, Shuman/C-1218-2015 FU USDA-ARS Project [5306-51530-019-00]; Danish Council for Strategic Research FX This study was supported in part by intramural USDA-ARS Project 5306-51530-019-00 (SHA) and the Danish Council for Strategic Research (RJM). USDA is an equal opportunity provider and employer. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 35 TC 33 Z9 33 U1 2 U2 33 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 9 PY 2014 VL 9 IS 12 AR e114881 DI 10.1371/journal.pone.0114881 PG 15 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AY3YM UT WOS:000347515300090 PM 25490712 ER PT J AU Best, NB Hartwig, T Budka, JS Bishop, BJ Brown, E Potluri, DPV Cooper, BR Premachandra, GS Johnston, CT Schulz, B AF Best, Norman B. Hartwig, Thomas Budka, Joshua S. Bishop, Brandon J. Brown, Elliot Potluri, Devi P. V. Cooper, Bruce R. Premachandra, Gnanasiri S. Johnston, Cliff T. Schulz, Burkhard TI Soilless Plant Growth Media Influence the Efficacy of Phytohormones and Phytohormone Inhibitors SO PLOS ONE LA English DT Article ID BRASSINOSTEROID BIOSYNTHESIS; FUNGICIDE PROPICONAZOLE; TRIAZOLE FUNGICIDES; PHYSICAL-PROPERTIES; GREEN-REVOLUTION; GIBBERELLIC ACID; RICE HULLS; PACLOBUTRAZOL; UNICONAZOLE; DRENCH AB Plant growth regulators, such as hormones and their respective biosynthesis inhibitors, are effective tools to elucidate the physiological function of phytohormones in plants. A problem of chemical treatments, however, is the potential for interaction of the active compound with the growth media substrate. We studied the interaction and efficacy of propiconazole, a potent and specific inhibitor of brassinosteroid biosynthesis, with common soilless greenhouse growth media for rice, sorghum, and maize. Many of the tested growth media interacted with propiconazole reducing its efficacy up to a hundred fold. To determine the molecular interaction of inhibitors with media substrates, Fourier Transform Infrared Spectroscopy and sorption isotherm analysis was applied. While mica clay substrates absorbed up to 1.3 mg of propiconazole per g substrate, calcined clays bound up to 12 mg of propiconazole per g substrate. The efficacy of the gibberellic acid biosynthesis inhibitor, uniconazole, and the most active brassinosteroid, brassinolide, was impacted similarly by the respective substrates. Conversely, gibberellic acid showed no distinct growth response in different media. Our results suggest that the reduction in efficacy of propiconazole, uniconazole, and brassinolide in bioassays when grown in calcined clay is caused by hydrophobic interactions between the plant growth regulators and the growth media. This was further confirmed by experiments using methanol-water solvent mixes with higher hydrophobicity values, which reduce the interaction of propiconazole and calcined clay. C1 [Best, Norman B.; Hartwig, Thomas; Budka, Joshua S.; Bishop, Brandon J.; Brown, Elliot; Schulz, Burkhard] Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. [Hartwig, Thomas] Carnegie Inst Sci, Dept Plant Biol, Stanford, CA 94305 USA. [Budka, Joshua S.] USDA ARS, Plant Soil & Nutr Res Unit, Ithaca, NY 14853 USA. [Potluri, Devi P. V.] Chicago State Univ, Dept Biol, Chicago, IL USA. [Cooper, Bruce R.] Purdue Univ, Bindley Biosci Ctr, W Lafayette, IN 47907 USA. [Premachandra, Gnanasiri S.; Johnston, Cliff T.] Purdue Univ, Dept Agron, W Lafayette, IN 47907 USA. RP Schulz, B (reprint author), Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA. EM bschulz1@umd.edu RI Best, Norman/G-1969-2015 OI Best, Norman/0000-0002-6572-5999 FU National Science Foundation CAREER Integrative Organismal Systems Grant [1054918] FX National Science Foundation CAREER Integrative Organismal Systems Grant 1054918 to BS (http://www.nsf.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 78 TC 0 Z9 0 U1 3 U2 12 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 8 PY 2014 VL 9 IS 12 AR e107689 DI 10.1371/journal.pone.0107689 PG 24 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AX4MX UT WOS:000346907600002 PM 25485677 ER PT J AU Ladinig, A Lunney, JK Souza, CJH Ashley, C Plastow, G Harding, JCS AF Ladinig, Andrea Lunney, Joan K. Souza, Carlos J. H. Ashley, Carolyn Plastow, Graham Harding, John C. S. TI Cytokine profiles in pregnant gilts experimentally infected with porcine reproductive and respiratory syndrome virus and relationships with viral load and fetal outcome SO VETERINARY RESEARCH LA English DT Article ID PLASMACYTOID DENDRITIC CELLS; IMMUNE-RESPONSES; PROINFLAMMATORY CYTOKINES; INTERLEUKIN-4 SUPPRESSES; PIGS; SWINE; EXPRESSION; GAMMA; VACCINATION; RECEPTOR AB In spite of extensive research, immunologic control mechanisms against Porcine Reproductive and Respiratory Syndrome virus (PRRSv) remain poorly understood. Cytokine responses have been exhaustively studied in nursery pigs and show contradictory results. Since no detailed reports on cytokine responses to PRRSv in pregnant females exist, the objectives of this study were to compare host cytokine responses between PRRSv-infected and non-infected pregnant gilts, and to investigate relationships between cytokine levels in infected gilts and viral load or fetal mortality rate. Serum samples and supernatants of peripheral blood mononuclear cells (PBMC) either stimulated with PRRSv or phorbol myristate acetate/Ionomycin (PMA/Iono) were analyzed for cytokines/chemokines: interleukins (IL) 1-beta (IL1 beta), IL4, IL8, IL10, IL12, chemokine ligand 2 (CCL2), interferon alpha (IFN alpha) and interferon gamma (IFN gamma). Three cytokines (IFN alpha, CCL2, IFN gamma) in gilt serum differed significantly in inoculated versus control gilts over time. In supernatants of PRRSv stimulated PBMC from PRRSv-infected gilts, levels of IFN alpha were significantly decreased, while IL8 secretion was significantly increased. PRRSv infection altered the secretion of all measured cytokines, with the exception of IFN alpha, from PBMC after mitogen stimulation, indicating a possible immunomodulatory effect of PRRSv. IFN alpha, CCL2, and IFN gamma in serum, and IFN gamma in supernatants of PMA/Iono stimulated PBMC were significantly associated with viral load in tissues, serum or both. However, only IFN alpha in supernatants of PRRSv stimulated PBMC was significantly associated with fetal mortality rate. We conclude that of the eight cytokines tested in this study IFN alpha was the best indicator of viral load and severity of reproductive PRRSv infection. C1 [Ladinig, Andrea; Ashley, Carolyn; Harding, John C. S.] Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. [Lunney, Joan K.; Souza, Carlos J. H.] ARS, USDA, Anim Parasit Dis Lab, Beltsville Agr Res Ctr, Beltsville, MD USA. [Souza, Carlos J. H.] EMBRAPA Pesca & Aquicultura, Palmas, TO, Brazil. [Plastow, Graham] Univ Alberta, Fac Agr Life & Environm Sci, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada. RP Ladinig, A (reprint author), Univ Saskatchewan, Western Coll Vet Med, Dept Large Anim Clin Sci, Saskatoon, SK S7N 0W0, Canada. EM andrea.ladinig@usask.ca OI Ladinig, Andrea/0000-0001-5037-7269 FU Genome Canada; Genome Prairie [2209-F] FX The authors wish to acknowledge the numerous technicians and students from the Western College of Veterinary Medicine, Vaccine and Infectious Disease Organization, Prairie Diagnostic Services, Inc. and the University of Alberta who assisted with this project. We offer special thanks to Samuel Abrams for the help in the development of the FMIA assay, and to Ian Dohoo for his guidance with statistical analyses. Pregnant gilts were provided and bred by Fast Genetics Inc., Spiritwood, Canada, with management support of Dawn Friesen, Connie Heisler, Donell Wingerter and Benny Mote. Funding for the project was generously provided by grants from Genome Canada and Genome Prairie (grant number 2209-F), with administrative support from Genome Alberta. NR 31 TC 8 Z9 8 U1 0 U2 5 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 0928-4249 EI 1297-9716 J9 VET RES JI Vet. Res. PD DEC 6 PY 2014 VL 45 AR 113 DI 10.1186/s13567-014-0113-8 PG 10 WC Veterinary Sciences SC Veterinary Sciences GA CO3MA UT WOS:000359060700001 PM 25479904 ER PT J AU Sobolev, AP Neelam, A Fatima, T Shukla, V Handa, AK Mattoo, AK AF Sobolev, Anatoly P. Neelam, Anil Fatima, Tahira Shukla, Vijaya Handa, Avtar K. Mattoo, Autar K. TI Genetic introgression of ethylene-suppressed transgenic tomatoes with higher-polyamines trait overcomes many unintended effects due to reduced ethylene on the primary rnetabolome SO FRONTIERS IN PLANT SCIENCE LA English DT Article DE ACC synthase; fruit ripening; LeACS2; metabolome; polyamines; spermidine; spermine; transgenics ID FRUIT-DEVELOPMENT; METABOLIC MEMORY; ANTISENSE GENE; BIOSYNTHESIS; REVEALS; QUALITY; PLANTS; TRANSCRIPT; RECEPTOR; JUICE AB Ethylene regulates a myriad physiological and biochemical processes in ripening fruits and is accepted as the ripening hormone for the climacteric fruits. However, its effects on metabolome and resulting fruit quality are not yet fully understood, particularly when some of the ripening-associated biochemical changes are independent of ethylene action. We have generated a homozygous transgenic tomato genotype (2AS-AS) that exhibits reduced ethylene production as a result of impaired expression of 1aminocyclopropane-1-carboxylate synthase 2 gene by its antisense RNA and had a longer shelf life. Double transgenic hybrid (2AS-AS x 579HO) developed through a genetic cross between 2AS-AS and 579HO (Mehta Mat, 2002) lines resulted in significantly higher ethylene production than either the WT or 2AS-AS fruit. To determine the effects of reduced ethylene and introgression of higher polyamines' trait, the metabolic profiles of ripening fruits from WT (556AZ), 2AS-AS, and 2AS-AS x 579HO lines were determined using 1H-NMR spectroscopy. The levels of Glu, Asp, AMP Adenosine, Nuc11, and Nucl2 increased during ripening of the WT fruit. The increases in Glu, Asp, and AMP levels were attenuated in 2AS-AS fruit but recovered in the double hybrid with higher ethylene and polyamine levels. The ripening-associated decreases in Ala, Tyr, Val, Ile, Phe, malate, and myo-inositol levels in the 2AS-AS line were not reversed in the double hybrid line suggesting a developmental/ripening regulated accumulation of these metabolites independent of ethylene. Significant increases in the levels of fumarate, formate, choline, Nuc11, and Nucl2 at most stages of ripening fruit were found in the double transgenic line due to introgression with higher-polyamines trait. Taken together these results show that the ripening-associated metabolic changes are both ethylene dependent and independent, and that the fruit metabolome is under the control of multiple regulators, including ethylene and polyamines. C1 [Sobolev, Anatoly P.] CNR, Inst Chem Methodol, Lab Magnet Resonance Annalaura Segre, Rome, Italy. [Neelam, Anil; Fatima, Tahira; Shukla, Vijaya; Mattoo, Autar K.] USDA ARS, Henry A Wallace Agr Res Ctr, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. [Handa, Avtar K.] Purdue Univ, Dept Hort, W Lafayette, IN 47907 USA. RP Mattoo, AK (reprint author), USDA ARS, Henry A Wallace Agr Res Ctr, Sustainable Agr Syst Lab, Beltsville, MD 20705 USA. EM autar.mattoo@ars.usda.gov OI SOBOLEV, ANATOLY/0000-0001-8709-7666 NR 40 TC 2 Z9 2 U1 0 U2 17 PU FRONTIERS RESEARCH FOUNDATION PI LAUSANNE PA PO BOX 110, LAUSANNE, 1015, SWITZERLAND SN 1664-462X J9 FRONT PLANT SCI JI Front. Plant Sci. PD DEC 5 PY 2014 VL 5 AR 632 DI 10.3389/fpls.2014.00632 PG 10 WC Plant Sciences SC Plant Sciences GA AY6UO UT WOS:000347701200001 PM 25538712 ER PT J AU Li, XB Jin, F Jin, L Jackson, A Huang, C Li, KH Shu, XL AF Li, Xiaobai Jin, Feng Jin, Liang Jackson, Aaron Huang, Cheng Li, Kehu Shu, Xiaoli TI Development of Cymbidium ensifolium genic-SSR markers and their utility in genetic diversity and population structure analysis in cymbidiums SO BMC GENETICS LA English DT Article DE Cymbidium ensifolium; Genic-SSR; Genetic diversity; Population structure ID PROLINE TRANSPORTER; MOLECULAR EVOLUTION; ARABIDOPSIS; CULTIVARS; BARLEY; RICE; DNA; POLYMORPHISM; SPECIFICITY; GENOMES AB Background: Cymbidium is a genus of 68 species in the orchid family, with extremely high ornamental value. Marker-assisted selection has proven to be an effective strategy in accelerating plant breeding for many plant species. Analysis of cymbidiums genetic background by molecular markers can be of great value in assisting parental selection and breeding strategy design, however, in plants such as cymbidiums limited genomic resources exist. In order to obtain efficient markers, we deep sequenced the C. ensifolium transcriptome to identify simple sequence repeats derived from gene regions (genic-SSR). Result: The 7,936 genic-SSR markers were identified. A total of 80 genic-SSRs were selected, and primers were designed according to their flanking sequences. Of the 80 genic-SSR primer sets, 62 were amplified in C. ensifolium successfully, and 55 showed polymorphism when cross-tested among 9 Cymbidium species comprising 59 accessions. Unigenes containing the 62 genic-SSRs were searched against Non-redundant (Nr), Gene Ontology database (GO), eukaryotic orthologous groups (KOGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. The search resulted in 53 matching Nr sequences, of which 39 had GO terms, 18 were assigned to KOGs, and 15 were annotated with KEGG. Genetic diversity and population structure were analyzed based on 55 polymorphic genic-SSR data among 59 accessions. The genetic distance averaged 0.3911, ranging from 0.016 to 0.618. The polymorphic index content (PIC) of 55 polymorphic markers averaged 0.407, ranging from 0.033 to 0.863. A model-based clustering analysis revealed that five genetic groups existed in the collection. Accessions from the same species were typically grouped together; however, C. goeringii accessions did not always form a separate cluster, suggesting that C. goeringii accessions were polyphyletic. Conclusion: The genic-SSR identified in this study constitute a set of markers that can be applied across multiple Cymbidium species and used for the evaluation of genetic relationships as well as qualitative and quantitative trait mapping studies. Genic-SSRs coupled with the functional annotations provided by the unigenes will aid in mapping candidate genes of specific function. C1 [Li, Xiaobai; Jin, Liang] Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China. [Jin, Feng] Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China. [Jackson, Aaron] ARS, USDA, Dale Bumpers Natl Rice Res Ctr, Stuttgart, AR 72160 USA. [Huang, Cheng] Shaoxing Cty Agr Bur, Agr Technol Extens Stn, Shaoxing 312000, Peoples R China. [Li, Kehu] Univ Hong Kong, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China. [Shu, Xiaoli] Zhejiang Univ, State Key Lab Rice Biol, Int Atom Energy Agcy Collaborating Ctr, Hangzhou 310029, Peoples R China. RP Li, XB (reprint author), Zhejiang Acad Agr Sci, Shiqiao Rd 139, Hangzhou 310021, Zhejiang, Peoples R China. EM hufanfan1982815@gmail.com; shuxl@zju.edu.cn RI Jin, Liang/C-4345-2011 OI Jin, Liang/0000-0001-6598-5144 FU National Basic Research Program - Nature Science Foundation of China [31201648]; Postdoctoral Science Foundation of China [2012 M521203]; Special Postdoctoral Science Foundation of China [2013 T60607]; Foundation for Selected Postdoctoral project of Zhejiang [Bsh1201032]; Qianjiang talents project [2013R10081]; Scientific and technical innovation promotion project of ZAAS [2012R05Y01E04] FX The authors thank Lin Biao for critical review, the lab of Professor Dianxing Wu for materials supply and technical support, and Chongbo Sun for a part of materials supply. This research was supported by the National Basic Research Program funded by the Nature Science Foundation of China (No. 31201648), the Postdoctoral Science Foundation of China (No. 2012 M521203), the Special Postdoctoral Science Foundation of China (No. 2013 T60607), and the Foundation for Selected Postdoctoral project of Zhejiang (Bsh1201032), the Qianjiang talents project (No. 2013R10081), and Scientific and technical innovation promotion project of ZAAS (2012R05Y01E04). NR 58 TC 1 Z9 1 U1 10 U2 33 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD DEC 5 PY 2014 VL 15 AR 124 DI 10.1186/s12863-014-0124-5 PG 14 WC Genetics & Heredity SC Genetics & Heredity GA AX9ET UT WOS:000347207300001 PM 25481640 ER PT J AU Bian, Y Yang, Q Balint-Kurti, PJ Wisser, RJ Holland, JB AF Bian, Yang Yang, Qin Balint-Kurti, Peter J. Wisser, Randall J. Holland, James B. TI Limits on the reproducibility of marker associations with southern leaf blight resistance in the maize nested association mapping population SO BMC GENOMICS LA English DT Article DE Quantitative trait loci; Nested association mapping; Disease resistance; Genome wide association study; Zea mays ID QUANTITATIVE TRAIT LOCI; INBRED LINE POPULATIONS; GENOME-WIDE ASSOCIATION; GENETIC ARCHITECTURE; DISEASE RESISTANCE; INNATE IMMUNITY; FLOWERING TIME; SALICYLIC-ACID; JOINT ANALYSIS; ARABIDOPSIS AB Background: A previous study reported a comprehensive quantitative trait locus (QTL) and genome wide association study (GWAS) of southern leaf blight (SLB) resistance in the maize Nested Association Mapping (NAM) panel. Since that time, the genomic resources available for such analyses have improved substantially. An updated NAM genetic linkage map has a nearly six-fold greater marker density than the previous map and the combined SNPs and read-depth variants (RDVs) from maize HapMaps 1 and 2 provided 28.5 M genomic variants for association analysis, 17 fold more than HapMap 1. In addition, phenotypic values of the NAM RILs were re-estimated to account for environment-specific flowering time covariates and a small proportion of lines were dropped due to genotypic data quality problems. Comparisons of original and updated QTL and GWAS results confound the effects of linkage map density, GWAS marker density, population sample size, and phenotype estimates. Therefore, we evaluated the effects of changing each of these parameters individually and in combination to determine their relative impact on marker-trait associations in original and updated analyses. Results: Of the four parameters varied, map density caused the largest changes in QTL and GWAS results. The updated QTL model had better cross-validation prediction accuracy than the previous model. Whereas joint linkage QTL positions were relatively stable to input changes, the residual values derived from those QTL models (used as inputs to GWAS) were more sensitive, resulting in substantial differences between GWAS results. The updated NAM GWAS identified several candidate genes consistent with previous QTL fine-mapping results. Conclusions: The highly polygenic nature of resistance to SLB complicates the identification of causal genes. Joint linkage QTL are relatively stable to perturbations of data inputs, but their resolution is generally on the order of tens or more Mbp. GWAS associations have higher resolution, but lower power due to stringent thresholds designed to minimize false positive associations, resulting in variability of detection across studies. The updated higher density linkage map improves QTL estimation and, along with a much denser SNP HapMap, greatly increases the likelihood of detecting SNPs in linkage with causal variants. We recommend use of the updated genetic resources and results but emphasize the limited repeatability of small-effect associations. C1 [Bian, Yang; Holland, James B.] N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. [Yang, Qin; Balint-Kurti, Peter J.] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA. [Balint-Kurti, Peter J.; Holland, James B.] USDA ARS, Plant Sci Res Unit, Raleigh, NC 27695 USA. [Wisser, Randall J.] Univ Delaware, Dept Plant & Soil Sci, Newark, DE 19716 USA. RP Holland, JB (reprint author), N Carolina State Univ, Dept Crop Sci, Raleigh, NC 27695 USA. EM Jim.Holland@ars.usda.gov OI Holland, James/0000-0002-4341-9675; Balint-Kurti, Peter/0000-0002-3916-194X FU United States National Science Foundation [IOS-1127076, IOS-0820619, IOS-1238014]; NSF [IOS-1127076, IOS-0820619, IOS-1238014]; Monsanto Co. fellowship FX We thank Sharon Mitchell, Jeff Glaubitz, Peter Bradbury, and Edward Buckler for the updated NAM linkage map. Research was supported by United States National Science Foundation grants IOS-1127076, IOS-0820619, and IOS-1238014. YB is a graduate student at NCSU supported by NSF grant IOS-0820619 and a Monsanto Co. fellowship. QY is a post-doctoral researcher at NCSU supported by NSF grant IOS-1127076. PBK and JH are research geneticists with the USDA-ARS. RW is faculty of University of Delaware. NSF grants IOS-1127076 supports research in the laboratories of PBK, RW, and JH. NSF grants IOS-0820619 and IOS-1238014 support research in the laboratory of JH. NR 54 TC 3 Z9 3 U1 3 U2 30 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD DEC 5 PY 2014 VL 15 AR 1068 DI 10.1186/1471-2164-15-1068 PG 15 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AX0WJ UT WOS:000346670300001 PM 25475173 ER PT J AU Bothwell, LD Selmants, PC Giardina, CP Litton, CM AF Bothwell, Lori D. Selmants, Paul C. Giardina, Christian P. Litton, Creighton M. TI Leaf litter decomposition rates increase with rising mean annual temperature in Hawaiian tropical montane wet forests SO PEERJ LA English DT Article DE Decomposition; Hawaii; Tropical wet forest; Climate change; Elevation gradient; Nitrogen cycling; Metrosideros polymorpha; Mean annual temperature (MAT); Leaf litter ID ORGANIC-MATTER; CARBON-DIOXIDE; STORAGE; METAANALYSIS; VARIABILITY; LIMITATION; DYNAMICS; GRADIENT; BALANCE; MODELS AB Decomposing litter in forest ecosystems supplies nutrients to plants, carbon to heterotrophic soil microorganisms and is a large source of CO2 to the atmosphere. Despite its essential role in carbon and nutrient cycling, the temperature sensitivity of leaf litter decay in tropical forest ecosystems remains poorly resolved, especially in tropical montane wet forests where the warming trend may be amplified compared to tropical wet forests at lower elevations. We quantified leaf litter decomposition rates along a highly constrained 5.2 degrees C mean annual temperature (MAT) gradient in tropical montane wet forests on the Island of Hawaii. Dominant vegetation, substrate type and age, soil moisture, and disturbance history are all nearly constant across this gradient, allowing us to isolate the effect of rising MAT on leaf litter decomposition and nutrient release. Leaf litter decomposition rates were a positive linear function of MAT, causing the residence time of leaf litter on the forest floor to decline by similar to 31 days for each 1 degrees C increase in MAT. Our estimate of the Q(10) temperature coefficient for leaf litter decomposition was 2.17, within the commonly reported range for heterotrophic organic matter decomposition (1.5-2.5) across a broad range of ecosystems. The percentage of leaf litter nitrogen (N) remaining after six months declined linearly with increasing MAT from similar to 88% of initial N at the coolest site to similar to 74% at the warmest site. The lack of net N immobilization during all three litter collection periods at all MAT plots indicates that N was not limiting to leaf litter decomposition, regardless of temperature. These results suggest that leaf litter decay in tropical montane wet forests may be more sensitive to rising MAT than in tropical lowland wet forests, and that increased rates of N release from decomposing litter could delay or prevent progressive N limitation to net primary productivity with climate warming. C1 [Bothwell, Lori D.] Univ Hawaii, Div Nat Sci, Hilo, HI 96720 USA. [Selmants, Paul C.; Litton, Creighton M.] Univ Hawaii Manoa, Dept Nat Resources & Environm Management, Honolulu, HI 96822 USA. [Giardina, Christian P.] USDA, Inst Pacific Isl Forestry, Pacific Southwest Res Stn, Forest Serv, Hilo, HI USA. RP Selmants, PC (reprint author), Univ Hawaii, Div Nat Sci, Hilo, HI 96720 USA. EM selmants@hawaii.edu RI Giardina, Christian/C-3120-2011; OI Giardina, Christian/0000-0002-3431-5073; Selmants, Paul/0000-0001-6211-3957 FU National Science Foundation through the Pacific Internship Program for Exploring Science (PIPES) [BIO-1005186]; Ecosystem Science Program [DEB-0816486]; USDA Forest Service, Institute of Pacific Islands Forestry, Pacific Southwest Research Station [09-JV-11272177-029, 12-JV-11272139-047]; College of Tropical Agriculture and Human Resources, the University of Hawaii at Manoa via the USDA National Institute of Food and Agriculture, Hatch and McIntire-Stennis Programs [HAW00132-H, HAW01127-H, HAW00188-M, HAW01123-M] FX This study was funded by the National Science Foundation through the Pacific Internship Program for Exploring Science (PIPES; BIO-1005186) and the Ecosystem Science Program (DEB-0816486). Additional funding was provided by the USDA Forest Service, Institute of Pacific Islands Forestry, Pacific Southwest Research Station (Research Joint Ventures 09-JV-11272177-029 and 12-JV-11272139-047) and the College of Tropical Agriculture and Human Resources, the University of Hawaii at Manoa via the USDA National Institute of Food and Agriculture, Hatch and McIntire-Stennis Programs (HAW00132-H, HAW01127-H, HAW00188-M, and HAW01123-M). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 32 TC 8 Z9 8 U1 8 U2 56 PU PEERJ INC PI LONDON PA 341-345 OLD ST, THIRD FLR, LONDON, EC1V 9LL, ENGLAND SN 2167-8359 J9 PEERJ JI PeerJ PD DEC 4 PY 2014 VL 2 AR e685 DI 10.7717/peerj.685 PG 12 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA AY5QU UT WOS:000347627700001 PM 25493213 ER PT J AU Christopherson, MR Dawson, JA Stevenson, DM Cunningham, AC Bramhacharya, S Weimer, PJ Kendziorski, C Suen, G AF Christopherson, Melissa R. Dawson, John A. Stevenson, David M. Cunningham, Andrew C. Bramhacharya, Shanti Weimer, Paul J. Kendziorski, Christina Suen, Garret TI Unique aspects of fiber degradation by the ruminal ethanologen Ruminococcus albus 7 revealed by physiological and transcriptomic analysis SO BMC GENOMICS LA English DT Article DE Ruminococcus albus; Cellulose utilization; Ethanol production ID CARBOHYDRATE-BINDING MODULES; CELL-SURFACE; CELLULOLYTIC BACTERIA; CONTINUOUS-CULTURE; ALBUS 7; PROTEIN; FERMENTATION; ADHESION; FLAVEFACIENS; EXPRESSION AB Background: Bacteria in the genus Ruminococcus are ubiquitous members of the mammalian gastrointestinal tract. In particular, they are important in ruminants where they digest a wide range of plant cell wall polysaccharides. For example, Ruminococcus albus 7 is a primary cellulose degrader that produces acetate usable by its bovine host. Moreover, it is one of the few organisms that ferments cellulose to form ethanol at mesophilic temperatures in vitro. The mechanism of cellulose degradation by R. albus 7 is not well-defined and is thought to involve pilin-like proteins, unique carbohydrate-binding domains, a glycocalyx, and cellulosomes. Here, we used a combination of comparative genomics, fermentation analyses, and transcriptomics to further clarify the cellulolytic and fermentative potential of R. albus 7. Results: A comparison of the R. albus 7 genome sequence against the genome sequences of related bacteria that either encode or do not encode cellulosomes revealed that R. albus 7 does not encode for most canonical cellulosomal components. Fermentation analysis of R. albus 7 revealed the ability to produce ethanol and acetate on a wide range of fibrous substrates in vitro. Global transcriptomic analysis of R. albus 7 grown at identical dilution rates on cellulose and cellobiose in a chemostat showed that this bacterium, when growing on cellulose, utilizes a carbohydrate-degrading strategy that involves increased transcription of the rare carbohydrate-binding module (CBM) family 37 domain and the tryptophan biosynthetic operon. Conclusions: Our data suggest that R. albus 7 does not use canonical cellulosomal components to degrade cellulose, but rather up-regulates the expression of CBM37-containing enzymes and tryptophan biosynthesis. This study contributes to a revised model of carbohydrate degradation by this key member of the rumen ecosystem. C1 [Christopherson, Melissa R.; Cunningham, Andrew C.; Bramhacharya, Shanti; Weimer, Paul J.; Suen, Garret] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA. [Stevenson, David M.; Weimer, Paul J.] USDA ARS, US Dairy Forage Res Ctr, Madison, WI 53706 USA. [Dawson, John A.; Kendziorski, Christina] Univ Wisconsin, Dept Biostat & Med Informat, Madison, WI 53706 USA. RP Suen, G (reprint author), Univ Wisconsin, Dept Bacteriol, 5159 Microbial Sci Bldg,1550 Linden Dr, Madison, WI 53706 USA. EM gsuen@wisc.edu OI Suen, Garret/0000-0002-6170-711X FU DOE BER Early Career Research Program [DE-SC0008104]; Wisconsin Bioenergy Initiative; NIH [GM102756]; USDA-Agricultural Research Service CRIS [3655-41000-007-00D]; DOE GLBRC/BACTER Post-doctoral Research Fellowship FX This work was supported by a DOE BER Early Career Research Program Award DE-SC0008104 and funding from the Wisconsin Bioenergy Initiative to GS, an NIH award GM102756 to CK, and a USDA-Agricultural Research Service CRIS project 3655-41000-007-00D to PJW. MRC was supported by a DOE GLBRC/BACTER Post-doctoral Research Fellowship. We thank J. M. Hackney for supplying glucomannan from Ochris tubers, P. Brumm for Soy polysaccharides, and members of the Suen lab for critical reading of the manuscript. NR 63 TC 6 Z9 6 U1 4 U2 37 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2164 J9 BMC GENOMICS JI BMC Genomics PD DEC 4 PY 2014 VL 15 AR UNSP 1066 DI 10.1186/1471-2164-15-1066 PG 13 WC Biotechnology & Applied Microbiology; Genetics & Heredity SC Biotechnology & Applied Microbiology; Genetics & Heredity GA AW6OS UT WOS:000346389300001 PM 25477200 ER PT J AU Wang, Y Thilmony, R Zhao, YJ Chen, GP Gu, YQ AF Wang, Yi Thilmony, Roger Zhao, Yunjun Chen, Guoping Gu, Yong Q. TI AIM: a comprehensive Arabidopsis interactome module database and related interologs in plants SO DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION LA English DT Article ID PROTEIN-INTERACTION NETWORK; DROSOPHILA-MELANOGASTER; C-ELEGANS; MAP; INFORMATION; COMPLEXES; UPDATE; GENES; TOOLS AB Systems biology analysis of protein modules is important for understanding the functional relationships between proteins in the interactome. Here, we present a comprehensive database named AIM for Arabidopsis (Arabidopsis thaliana) interactome modules. The database contains almost 250 000 modules that were generated using multiple analysis methods and integration of microarray expression data. All the modules in AIM are well annotated using multiple gene function knowledge databases. AIM provides a user-friendly interface for different types of searches and offers a powerful graphical viewer for displaying module networks linked to the enrichment annotation terms. Both interactive Venn diagram and power graph viewer are integrated into the database for easy comparison of modules. In addition, predicted interologs from other plant species (homologous proteins from different species that share a conserved interaction module) are available for each Arabidopsis module. AIM is a powerful systems biology platform for obtaining valuable insights into the function of proteins in Arabidopsis and other plants using the modules of the Arabidopsis interactome. C1 [Wang, Yi; Gu, Yong Q.] ARS, USDA, Western Reg Res Ctr, Genom & Gene Discovery Res Unit, Albany, CA 94710 USA. [Wang, Yi] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Wang, Yi; Chen, Guoping] Chongqing Univ, Bioengn Coll, Chongqing 400030, Peoples R China. [Thilmony, Roger] ARS, USDA, Western Reg Res Ctr, Crop Improvement & Utilizat Res Unit, Albany, CA 94710 USA. [Zhao, Yunjun] Univ Calif Berkeley, Energy Biosci Inst, Berkeley, CA 94720 USA. RP Gu, YQ (reprint author), ARS, USDA, Western Reg Res Ctr, Genom & Gene Discovery Res Unit, Albany, CA 94710 USA. EM Yi.Wang@ars.usda.gov; Yong.Gu@ars.usda.gov FU US National Science Foundation [IOS 0822100]; US Department of Agriculture, Agriculture Research Service CRIS [5325-21000-014]; [0822100] FX US National Science Foundation (grant number IOS 0822100) and US Department of Agriculture, Agriculture Research Service CRIS project 5325-21000-014. Funding open access charge: 0822100. NR 30 TC 2 Z9 2 U1 1 U2 7 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1758-0463 J9 DATABASE-OXFORD JI Database PD DEC 4 PY 2014 AR bau117 DI 10.1093/database/bau117 PG 8 WC Mathematical & Computational Biology SC Mathematical & Computational Biology GA AU9GD UT WOS:000345898800001 ER PT J AU Gai, XP Wang, HY Liu, J Zhai, LM Liu, S Ren, TZ Liu, HB AF Gai, Xiapu Wang, Hongyuan Liu, Jian Zhai, Limei Liu, Shen Ren, Tianzhi Liu, Hongbin TI Effects of Feedstock and Pyrolysis Temperature on Biochar Adsorption of Ammonium and Nitrate SO PLOS ONE LA English DT Article ID BLACK CARBON; NUTRIENT AVAILABILITY; SOIL AMENDMENT; SORPTION; WATER; RESIDUE; MANURE; SULFAMETHOXAZOLE; PHOSPHATE; DYNAMICS AB Biochar produced by pyrolysis of biomass can be used to counter nitrogen (N) pollution. The present study investigated the effects of feedstock and temperature on characteristics of biochars and their adsorption ability for ammonium N (NH4+-N) and nitrate N (NO3--N). Twelve biochars were produced from wheat-straw (W-BC), corn-straw (C-BC) and peanut-shell (P-BC) at pyrolysis temperatures of 400, 500, 600 and 700 degrees C. Biochar physical and chemical properties were determined and the biochars were used for N sorption experiments. The results showed that biochar yield and contents of N, hydrogen and oxygen decreased as pyrolysis temperature increased from 400 degrees C to 700 degrees C, whereas contents of ash, pH and carbon increased with greater pyrolysis temperature. All biochars could sorb substantial amounts of NH4+-N, and the sorption characteristics were well fitted to the Freundlich isotherm model. The ability of biochars to adsorb NH4+-N followed: C-BC. P-BC. W-BC, and the adsorption amount decreased with higher pyrolysis temperature. The ability of C-BC to sorb NH4+-N was the highest because it had the largest cation exchange capacity (CEC) among all biochars (e.g., C-BC400 with a CEC of 38.3 cmol kg(-1) adsorbed 2.3 mg NH4+-N g(-1) in solutions with 50 mg NH4+ L-1). Compared with NH4+-N, none of NO3 -N was adsorbed to biochars at different NO3 concentrations. Instead, some NO3- -N was even released from the biochar materials. We conclude that biochars can be used under conditions where NH4+-N (or NH3) pollution is a concern, but further research is needed in terms of applying biochars to reduce NO3--N pollution. C1 [Gai, Xiapu; Wang, Hongyuan; Liu, Jian; Zhai, Limei; Liu, Shen; Liu, Hongbin] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Nonpoint Source Pollut Control, Beijing 100193, Peoples R China. [Liu, Jian] ARS, USDA, Pasture Syst & Watershed Management Res Unit, University Pk, PA USA. [Ren, Tianzhi] Minist Agr, Inst Agroenvironm Protect, Tianjin, Peoples R China. RP Liu, HB (reprint author), Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Nonpoint Source Pollut Control, Beijing 100193, Peoples R China. EM liuhongbin@caas.cn RI Liu, Jian/P-4050-2014 OI Liu, Jian/0000-0003-4199-1296 FU National Natural Science Foundation of China [41301311, 41203072]; Special Fund for Agro-scientific Research in the Public Interest [201303095-10] FX Funding: This study was financially supported by the National Natural Science Foundation of China (41301311), the Special Fund for Agro-scientific Research in the Public Interest (201303095-10), and the National Natural Science Foundation of China (41203072). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. NR 42 TC 20 Z9 25 U1 15 U2 132 PU PUBLIC LIBRARY SCIENCE PI SAN FRANCISCO PA 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA SN 1932-6203 J9 PLOS ONE JI PLoS One PD DEC 3 PY 2014 VL 9 IS 12 AR e113888 DI 10.1371/journal.pone.0113888 PG 19 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CA7WS UT WOS:000349128700050 PM 25469875 ER PT J AU Seiber, JN Coats, J Duke, SO Gross, AD AF Seiber, James N. Coats, Joel Duke, Stephen O. Gross, Aaron D. TI Biopesticides: State of the Art and Future Opportunities SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article ID NATURAL-PRODUCTS; RNA INTERFERENCE; CROP PROTECTION; STRANDED-RNA; HERBICIDE; PESTICIDES; PLANTS; TOXAPHENE; DISCOVERY; IMPACT AB The use of biopesticides and related alternative management products is increasing. New tools, including semiochemicals and plant-incorporated protectants (PIPs), as well as botanical and microbially derived chemicals, are playing an increasing role in pest management, along with plant and animal genetics, biological control, cultural methods, and newer synthetics. The goal of this Perspective is to highlight promising new biopesticide research and development (R&D), based upon recently published work and that presented in the American Chemical Society (ACS) symposium Biopesticides: State of the Art and Future Opportunities, as well as the authors own perspectives. Although the focus is on biopesticides, included in this Perspective is progress with products exhibiting similar characteristics, namely those naturally occurring or derived from natural products. These are target specific, of low toxicity to nontarget organisms, reduced in persistence in the environment, and potentially usable in organic agriculture. Progress is being made, illustrated by the number of biopesticides and related products in the registration pipeline, yet major commercial opportunities exist for new bioherbicides and bionematicides, in part occasioned by the emergence of weeds resistant to glyphosate and the phase-out of methyl bromide. The emergence of entrepreneurial start-up companies, the U.S. Environmental Protection Agency (EPA) fast track for biopesticides, and the availability of funding for registration-related R&D for biorational pesticides through the U.S. IR-4 program provide incentives for biopesticide development, but an expanded effort is warranted both in the United States and worldwide to support this relatively nascent industry. C1 [Seiber, James N.] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA. [Coats, Joel] Iowa State Univ, Dept Entomol, Ames, IA 50011 USA. [Duke, Stephen O.] ARS, NPURU, USDA, University, MS 38677 USA. [Gross, Aaron D.] Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA. RP Seiber, JN (reprint author), Univ Calif Davis, Dept Environm Toxicol, One Shields Ave, Davis, CA 95616 USA. EM jnseiber@ucdavis.edu NR 53 TC 28 Z9 30 U1 15 U2 132 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 3 PY 2014 VL 62 IS 48 BP 11613 EP 11619 DI 10.1021/jf504252n PG 7 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AU9AH UT WOS:000345883800001 PM 25406111 ER PT J AU Chen, ZB Tonnis, B Morris, B Wang, RB Zhang, AL Pinnow, D Wang, ML AF Chen, Zhenbang Tonnis, Brandon Morris, Brad Wang, Richard B. Zhang, Amy L. Pinnow, David Wang, Ming Li TI Variation in Seed Fatty Acid Composition and Sequence Divergence in the FAD2 Gene Coding Region between Wild and Cultivated Sesame SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE sesame; fatty acid compsition; gene for fatty acid desaturase (FAD2); nucleotide diversity ID SEBHLH TRANSCRIPTION FACTOR; DESATURASE GENES; SEFAD2 PROMOTER; OIL CONTENT; INDICUM L.; EXPRESSION; RESVERATROL; COLLECTION; ELEMENTS; CDNA AB Sesame germplasm harbors genetic diversity which can be useful for sesame improvement in breeding programs. Seven accessions with different levels of oleic acid were selected from the entire USDA sesame germplasm collection (1232 accessions) and planted for morphological observation and re-examination of fatty acid composition. The coding region of the FAD2 gene for fatty acid desaturase (FAD) in these accessions was also sequenced. Cultivated sesame accessions flowered and matured earlier than the wild species. The cultivated sesame seeds contained a significantly higher percentage of oleic acid (40.4%) than the seeds of the wild species (26.1%). Nucleotide polymorphisms were identified in the FAD2 gene coding region between wild and cultivated species. Some nucleotide polymorphisms led to amino acid changes, one of which was located in the enzyme active site and may contribute to the altered fatty acid composition. Based on the morphology observation, chemical analysis, and sequence analysis, it was determined that two accessions were misnamed and need to be reclassified. The results obtained from this study are useful for sesame improvement in molecular breeding programs. C1 [Chen, Zhenbang] Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. [Tonnis, Brandon; Morris, Brad; Pinnow, David; Wang, Ming Li] USDA ARS, Plant Genet Resources Conservat Unit, Griffin, GA 30223 USA. [Wang, Richard B.; Zhang, Amy L.] Univ Georgia, Young Scholar Program, Griffin, GA 30223 USA. RP Chen, ZB (reprint author), Univ Georgia, Dept Crop & Soil Sci, Griffin, GA 30223 USA. EM zchen@uga.edu; mingli.wang@ars.usda.gov NR 25 TC 0 Z9 1 U1 5 U2 23 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 3 PY 2014 VL 62 IS 48 BP 11706 EP 11710 DI 10.1021/jf503648b PG 5 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AU9AH UT WOS:000345883800012 PM 25386691 ER PT J AU Smith, DJ Ernst, W Giddings, JM AF Smith, D. J. Ernst, W. Giddings, J. M. TI Distribution and Chemical Fate of Cl-36-Chlorine Dioxide Gas during the Fumigation of Tomatoes and Cantaloupe SO JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY LA English DT Article DE cantaloupe; chlorate; chlorine dioxide; chloroxyanion; food safety; fumigation; perchlorate; tomato ID GASEOUS CHLORINE DIOXIDE; ESCHERICHIA-COLI O157-H7; KILLING SALMONELLA; AQUEOUS-SOLUTION; SODIUM-CHLORITE; PHOTODECOMPOSITION; STRAWBERRIES; IRRADIATION; METABOLISM; SANITIZER AB The distribution and chemical fate of Cl-36-ClO2 gas subsequent to fumigation of tomatoes or cantaloupe was investigated as were major factors that affect the formation of chloroxyanion byproducts. Approximately 22% of the generated Cl-36-ClO2 was present on fumigated tomatoes after a 2 h exposure to approximately 5 mg of Cl-36-ClO2. A water rinse removed 14% of the radiochlorine while tomato homogenate contained similar to 63% of the tomato radioactivity; 24% of the radiochlorine was present in the tomato stem scar area. Radioactivity in tomato homogenate consisted of Cl-36-chloride (>= 80%), Cl-36-chlorate (5 to 19%), and perchlorate (0.5 to 1.4%). In cantaloupe, 55% of the generated Cl-36-ClO2 was present on melons fumigated with 100 mg of Cl-36-ClO2 for a 2 h period. Edible cantaloupe flesh contained no detectable radioactive residue (LOQ = 0.3 to 0.4 mu g/g); >99.9% of radioactivity associated with cantaloupe was on the inedible rind, with <0.1% associated with the seed bed. Rind radioactivity was present as Cl-36-chloride (similar to 86%), chlorate (similar to 13%), and perchlorate (similar to 0.6%). Absent from tomatoes and cantaloupe were Cl-36-chlorite residues. Follow-up studies have shown that chlorate and perchlorate formation can be completely eliminated by protecting fumigation chambers from light sources. C1 [Smith, D. J.; Giddings, J. M.] ARS, Biosci Res Lab, USDA, Fargo, ND 58102 USA. [Ernst, W.] ICA TriNova LLC, Newnan, GA 30263 USA. RP Smith, DJ (reprint author), ARS, Biosci Res Lab, USDA, 1605 Albrecht Blvd, Fargo, ND 58102 USA. EM david.j.smith@ars.usda.gov FU ICA-TriNova [58-5442-1-431]; USDA ARS [5442-32000-014] FX This study was collaboratively funded by ICA-TriNova (Agreement No. 58-5442-1-431) and the USDA ARS (Project No. 5442-32000-014). NR 27 TC 3 Z9 3 U1 2 U2 8 PU AMER CHEMICAL SOC PI WASHINGTON PA 1155 16TH ST, NW, WASHINGTON, DC 20036 USA SN 0021-8561 EI 1520-5118 J9 J AGR FOOD CHEM JI J. Agric. Food Chem. PD DEC 3 PY 2014 VL 62 IS 48 BP 11756 EP 11766 DI 10.1021/jf1504097p PG 11 WC Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology SC Agriculture; Chemistry; Food Science & Technology GA AU9AH UT WOS:000345883800018 PM 25409284 ER PT J AU Krishnamurthy, P Tsukamoto, C Takahashi, Y Hongo, Y Singh, RJ Lee, JD Chung, G AF Krishnamurthy, Panneerselvam Tsukamoto, Chigen Takahashi, Yuya Hongo, Yuji Singh, Ram J. Lee, Jeong Dong Chung, Gyuhwa TI Comparison of saponin composition and content in wild soybean (Glycine soja Sieb. and Zucc.) before and after germination SO BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY LA English DT Article DE wild soybean; Glycine soja; germination; soyasaponins; triterpene glycosides ID LETTUCE ROOTS; SEED; SOYASAPOGENOL; POLYMORPHISM; INHERITANCE; COMPONENTS; ALLELE; PLANT AB Eight wild soybean accessions with different saponin phenotypes were used to examine saponin composition and relative saponin quantity in various tissues of mature seeds and two-week-old seedlings by LC-PDA/MS/MS. Saponin composition and content were varied according to tissues and accessions. The average total saponin concentration in 1 g mature dry seeds of wild soybean was 16.08 +/- 3.13 mu mol. In two-week-old seedlings, produced from 1 g mature seeds, it was 27.94 +/- 6.52 mu mol. Group A saponins were highly concentrated in seed hypocotyl (4.04 +/- 0.71 mu mol). High concentration of DDMP saponins (7.37 +/- 5.22 mu mol) and Sg-6 saponins (2.19 +/- 0.59 mu mol) was found in cotyledonary leaf. In seedlings, the amounts of group A and Sg-6 saponins reduced 2.3- and 1.3-folds, respectively, while DDMP + B + E saponins increased 2.5-fold than those of mature seeds. Our findings show that the group A and Sg-6 saponins in mature seeds were degraded and/or translocated by germination whereas DDMP saponins were newly synthesized. C1 [Krishnamurthy, Panneerselvam; Chung, Gyuhwa] Chonnam Natl Univ, Dept Biotechnol, Yeosu, South Korea. [Tsukamoto, Chigen; Takahashi, Yuya; Hongo, Yuji] Iwate Univ, Dept Appl Biol Chem, Morioka, Iwate 020, Japan. [Singh, Ram J.] Univ Illinois, USDA, ARS, Natl Soybean Res Lab, Urbana, IL 61801 USA. [Lee, Jeong Dong] Kyungpook Natl Univ, Sch Appl Biosci, Taegu, South Korea. RP Tsukamoto, C (reprint author), Iwate Univ, Dept Appl Biol Chem, Morioka, Iwate 020, Japan. EM chigen@iwate-u.ac.jp; chung@chonnam.ac.kr FU Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2010-0013600] FX This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0013600). NR 29 TC 2 Z9 2 U1 2 U2 30 PU TAYLOR & FRANCIS LTD PI ABINGDON PA 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND SN 0916-8451 EI 1347-6947 J9 BIOSCI BIOTECH BIOCH JI Biosci. Biotechnol. Biochem. PD DEC 2 PY 2014 VL 78 IS 12 BP 1988 EP 1996 DI 10.1080/09168451.2014.946389 PG 9 WC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Chemistry, Applied; Food Science & Technology SC Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Chemistry; Food Science & Technology GA AU3HZ UT WOS:000345504400003 PM 25127168 ER PT J AU Asah, ST Guerry, AD Blahna, DJ Lawler, JJ AF Asah, Stanley T. Guerry, Anne D. Blahna, Dale J. Lawler, Joshua J. TI Perception, acquisition and use of ecosystem services: Human behavior, and ecosystem management and policy implications SO ECOSYSTEM SERVICES LA English DT Article DE Indirect ecosytem services; Motivations; Behavioral compliance; Sense of place; Community; Pro-environmental behavior ID MILLENNIUM ASSESSMENT; FUNCTIONAL-APPROACH; SOCIAL-PSYCHOLOGY; PLACE ATTACHMENT; INNER-CITY; CONNECTEDNESS; COMMUNITIES; ENGAGEMENT; FOREST AB Ecosystem services, fundamental to livelihoods and well-being, are reshaping environmental management and policy. However, the behavioral dimensions of ecosystem services and the responses of ordinary people to the management of those services, is less well understood. The ecosystem services framework lends itself to understanding the relationship between ecosystems and human behavior. Ecosystem services, according to the psychological theory of motivational functionalism, are motivations the personal and social processes that initiate, direct and sustain human action. Thus, how people perceive, acquire and use ecosystem services influences the initiation, direction, and intensity of their behaviors. Profound understanding of how people perceive, acquire and use ecosystem services can help influence behavioral compliance with management and policy prescriptions. We use focus group interviewing to illustrate how ecosystem services relate to human behavior. Results show that people perceive, acquire and use indirect benefits while acquiring direct ecosystem services. Understanding indirect benefits has implications for the constitution and regulation of human behavior through ecosystem management and policy. Perceived ecosystem benefits, expressed in people's own words and from their own frames of reference, can facilitate better valuation of ecosystem services and setting of prices, compliance with ecosystem management and policy directives, recruitment and retention of ecosystem stewards, development of use policies, enhancement of user experiences, and encouragement of pro environmental attitudes and behaviors. (C) 2014 Elsevier B.V. All rights reserved. C1 [Asah, Stanley T.; Lawler, Joshua J.] Univ Washington, Coll Environm, Sch Environm & Forest Sci, Seattle, WA 98195 USA. [Guerry, Anne D.] Univ Minnesota, Stanford Univ, Nat Capital Project, TNC, Seattle, WA 98195 USA. [Guerry, Anne D.] WWF, Seattle, WA 98195 USA. [Blahna, Dale J.] US Forest Serv, USDA, Pacific NW Res Stn, Seattle, WA 98103 USA. RP Asah, ST (reprint author), Univ Washington, Coll Environm, Sch Environm & Forest Sci, Box 352100, Seattle, WA 98195 USA. EM stasah@uw.edu; anne.guerry@stanford.edu; dblahna@fs.fed.us; jlawler@uw.edu FU USDA Forest Service, Pacific Northwest Research Station FX The authors extend their gratitude to the interviewees of the Warm Springs Tribe. We thank Mr. I, Bell and Mr. D. French for help with participant recruitment, and interview moderation and transcribing. We are grateful for the insightful comments from the anonymous reviewers and the editors of this journal. We thank the USDA Forest Service, Pacific Northwest Research Station, for funding this study. NR 46 TC 14 Z9 14 U1 12 U2 33 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-0416 J9 ECOSYST SERV JI Ecosyst. Serv. PD DEC PY 2014 VL 10 SI SI BP 180 EP 186 DI 10.1016/j.ecoser.2014.08.003 PG 7 WC Ecology; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA CU6SD UT WOS:000363662700020 ER PT J AU Connolly, JJT Svendsen, ES Fisher, DR Campbell, LK AF Connolly, James J. T. Svendsen, Erika S. Fisher, Dana R. Campbell, Lindsay K. TI Networked governance and the management of ecosystem services: The case of urban environmental stewardship in New York City SO ECOSYSTEM SERVICES LA English DT Article DE Urban environmental stewardship; Hybrid governance; Network governance; Urban ecosystem services ID SOCIAL-ECOLOGICAL SYSTEMS; PERSPECTIVE; RESILIENCE; ORGANIZATIONS; FRAMEWORK AB Urban environmental stewardship groups have become an essential component of the governance structure that regulates ecosystem services in cities. New York City is one example where these groups have grown rapidly in number, size, and visibility since the 19705. In this article, we combine quantitative survey data with qualitative interview data to examine the structure and development of the governance network that has grown around the management of urban ecosystem services in New York City. We find that the network is organized according to ecological function and geography. We find as well, that certain historical conditions led to the development of a hybrid institutional form with regard to management of ecosystem services in the city. We discuss the implications of this hybrid networked governance structure in New York City and what it might mean for further cross-disciplinary research around ecosystem service governance. (C) 2014 Elsevier B.V. All rights reserved. C1 [Connolly, James J. T.] Northeastern Univ, Sch Publ Policy & Urban Affairs, Boston, MA 02115 USA. [Svendsen, Erika S.; Campbell, Lindsay K.] US Forest Serv, USDA, No Res Stn, New York, NY USA. [Fisher, Dana R.] Univ Maryland, Sociol, College Pk, MD 20742 USA. [Fisher, Dana R.] Univ Maryland, Program Soc & Environm, College Pk, MD 20742 USA. RP Connolly, JJT (reprint author), Northeastern Univ, Publ Policy & Urban Affairs, 310 Renaissance Pk,360 Huntington Ave, Boston, MA 02115 USA. EM j.connolly@neu.edu FU U.S. National Science Foundation [DEB 0948451]; USDA Forest Service FX This project was funded by a grant from the U.S. National Science Foundation (DEB 0948451) as well as with support from the USDA Forest Service. NR 49 TC 6 Z9 6 U1 10 U2 15 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 2212-0416 J9 ECOSYST SERV JI Ecosyst. Serv. PD DEC PY 2014 VL 10 SI SI BP 187 EP 194 DI 10.1016/j.ecoser.2014.08.005 PG 8 WC Ecology; Environmental Sciences; Environmental Studies SC Environmental Sciences & Ecology GA CU6SD UT WOS:000363662700021 ER PT J AU Middleton, JR Saeman, A Fox, LK Lombard, J Hogan, JS Smith, KL AF Middleton, John R. Saeman, Anne Fox, Larry K. Lombard, Jason Hogan, Joe S. Smith, K. Larry TI The National Mastitis Council: A Global Organization for Mastitis Control and Milk Quality, 50 Years and Beyond SO JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA LA English DT Article DE Mastitis; Bovine; Milk quality; National mastitis council ID MANAGEMENT; PROTOCOLS; EFFICACY AB The National Mastitis Council was founded in 1961 based on the desire of a forward-thinking group of individuals to bring together "all forces of organized agriculture in the United States to combat, through every practical device, the mastitis threat to the Nation's health and food safety". What started as a small organization focused on mastitis of dairy cattle in the United States has grown into a global organization for mastitis and milk quality. Over the last 50-plus years the concerted efforts of the membership have led to the synthesis and dissemination of a considerable body of knowledge regarding udder health, milk quality, and food safety which has improved dairy cattle health and well-being and farm productivity. C1 [Middleton, John R.] Univ Missouri, Dept Vet Med & Surg, Columbia, MO 65211 USA. [Saeman, Anne] Natl Mastitis Council Inc, Verona, WI 53593 USA. [Fox, Larry K.] Washington State Univ, Dept Vet Clin Sci, Pullman, WA 99164 USA. [Lombard, Jason] USDA, Natl Anim Hlth Monitoring Syst, Ft Collins, CO 80526 USA. [Hogan, Joe S.; Smith, K. Larry] Ohio State Univ, Ohio Agr Res & Dev Ctr, Wooster, OH 44691 USA. RP Middleton, JR (reprint author), Univ Missouri, Dept Vet Med & Surg, 900 East Campus Dr, Columbia, MO 65211 USA. EM middletonjr@missouri.edu NR 22 TC 2 Z9 2 U1 3 U2 8 PU SPRINGER/PLENUM PUBLISHERS PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 1083-3021 EI 1573-7039 J9 J MAMMARY GLAND BIOL JI J. Mammary Gland Biol. Neoplasia PD DEC PY 2014 VL 19 IS 3-4 BP 241 EP 251 DI 10.1007/s10911-014-9328-6 PG 11 WC Oncology; Endocrinology & Metabolism; Physiology SC Oncology; Endocrinology & Metabolism; Physiology GA CN0VM UT WOS:000358132100001 PM 25524293 ER PT J AU Meza, JS Schetelig, MF Zepeda-Cisneros, CS Handler, AM AF Salvador Meza, J. Schetelig, Marc F. Silvia Zepeda-Cisneros, C. Handler, Alfred M. TI Male-specific Y-linked transgene markers to enhance biologically-based control of the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae) SO BMC GENETICS LA English DT Article ID GERM-LINE TRANSFORMATION; DROSOPHILA-MELANOGASTER; GENE-EXPRESSION; PIGGYBAC TRANSPOSON; CERATITIS-CAPITATA; SPERM MARKING; SEXING STRAIN; CHROMOSOME; PROMOTER; VECTOR AB Background: Reliable marking systems are critical to the prospective field release of transgenic insect strains. This is to unambiguously distinguish released insects from wild insects in the field that are collected in field traps, and tissue-specific markers, such as those that are sperm-specific, have particular uses such as identifying wild females that have mated with released males. For tephritid fruit flies such as the Mexican fruit fly, Anastrepha ludens, polyubiquitin-regulated fluorescent protein body markers allow transgenic fly identification, and fluorescent protein genes regulated by the spermatocyte-specific beta 2-tubulin promoter effectively mark sperm. For sterile male release programs, both marking systems can be made male-specific by linkage to the Y chromosome. Results: An A. ludens wild type strain was genetically transformed with a piggyBac vector, pBXL{PUbnlsEGFP, As beta 2tub-DsRed.T3}, having the polyubiquitin-regulated EGFP body marker, and the b2-tubulin-regulated DsRed. T3 sperm-specific marker. Autosomal insertion lines effectively expressed both markers, but a single Y-linked insertion (Y-EGFP strain) expressed only PUbnlsEGFP. This insertion was remobilized by transposase helper injection, which resulted in three new autosomal insertion lines that expressed both markers. This indicated that the original Y-linked As beta 2tub-DsRed. T3 marker was functional, but specifically suppressed on the Y chromosome. The PUbnlsEGFP marker remained effective however, and the Y-EGFP strain was used to create a sexing strain by translocating the wild type allele of the black pupae (bp(1)) gene onto the Y, which was then introduced into the bp(-) mutant strain. This allows the mechanical separation of mutant female black pupae from male brown pupae, that can be identified as adults by EGFP fluorescence. Conclusions: A Y-linked insertion of the pBXL{PUbnlsEGFP, As beta 2tub-DsRed. T3} transformation vector in A. ludens resulted in male-specific expression of the EGFP fluorescent protein marker, and was integrated into a black pupae translocation sexing strain (T(Y-EGFP/bp(+)), allowing the identification of male adults when used in sterile male release programs for population control. A unique observation was that expression of the As beta 2tub-DsRed. T3 sperm-specific marker, which was functional in autosomal insertions, was specifically suppressed in the Y-linked insertion. This may relate to the Y chromosomal regulation of male-specific germ-line genes in Drosophila. C1 [Salvador Meza, J.; Silvia Zepeda-Cisneros, C.] SAGARPA IICA, Programa Moscafrut, Metapa de Dominguez 30860, Chiapas, Mexico. [Salvador Meza, J.] Univ Veracruzana, Inst Biotecnol & Ecol Aplicada INBIOT, Xalapa 91000, Veracruz, Mexico. [Schetelig, Marc F.; Handler, Alfred M.] USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. [Schetelig, Marc F.] Univ Giessen, Inst Phytopathol & Appl Zool, D-35390 Giessen, Germany. RP Handler, AM (reprint author), USDA ARS, Ctr Med Agr & Vet Entomol, Gainesville, FL 32608 USA. EM al.handler@ars.usda.gov FU Programa Moscafrut/ SAGARPA-IICA; Consejo Nacional de Ciencia y Tecnologia (CONACyT) [229669]; Emmy Noether program of the German Research Foundation [SCHE 1833/1-1]; LOEWE Center for Insect Biotechnology and Bioresources; USDA-NIFA-Biotechnology Risk Assessment Grant Program [2011-39211-30769]; International Atomic Energy Agency FX This research benefited from discussions at the International Atomic Energy Agency Coordinated Research Project, "Development and Evaluation of Improved Strains of Insect Pests for SIT", and was supported by the Programa Moscafrut/ SAGARPA-IICA, the 'Consejo Nacional de Ciencia y Tecnologia (CONACyT)' (no. 229669; to JSM, SZ-C), the Emmy Noether program of the German Research Foundation (SCHE 1833/1-1; MFS) and the LOEWE Center for Insect Biotechnology and Bioresources (to MFS), and the USDA-NIFA-Biotechnology Risk Assessment Grant Program (no. 2011-39211-30769; to AMH). We also thank Tanja Rehling for excellent technical assistance.; Publication of this supplement was funded by the International Atomic Energy Agency. The peer review process for articles published in this supplement was overseen by the Supplement Editors in accordance with BioMed Central's peer review guidelines for supplements. The Supplement Editors declare that they have no competing interests. NR 32 TC 1 Z9 1 U1 3 U2 6 PU BIOMED CENTRAL LTD PI LONDON PA 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND SN 1471-2156 J9 BMC GENET JI BMC Genet. PD DEC 1 PY 2014 VL 15 SU 2 AR S4 DI 10.1186/1471-2156-15-S2-S4 PG 7 WC Genetics & Heredity SC Genetics & Heredity GA CH4CW UT WOS:000353980100005 PM 25472528 ER PT J AU Yakubova, G Wielopolski, L Kavetskiy, A Torbert, HA Prior, SA AF Yakubova, Galina Wielopolski, Lucian Kavetskiy, Aleksandr Torbert, H. Allen Prior, Stephen A. TI Field Testing a Mobile Inelastic Neutron Scattering System to Measure Soil Carbon SO SOIL SCIENCE LA English DT Article DE Carbon; soil analysis; inelastic neutron scattering; thermo-neutron capture; neutron generator ID GAMMA-RAY SPECTROSCOPY; SPECTRA AB Cropping history in conjunction with soil management practices can have a major impact on the amount of organic carbon stored in soil. Current methods of assessing soil carbon based on soil coring and subsequent processing procedures before laboratory analysis are labor intensive and time-consuming. Development of alternative methods that can make in situ field measurements of soil carbon is needed to successfully evaluate management practices in a timely manner. The robust design, field testing procedure, and results of measuring soil carbon in situ using a mobile inelastic neutron scattering (MINS) system are described. A method of MINS spectra data processing that gives more accurate peak area determination compared with the traditional "trapezoidal" method is described. The MINS reliable autonomous operation for 29 h per charge cycle was demonstrated in the field. For comparison, soil cores were also collected for laboratory carbon analysis using the dry combustion technique. Soil carbon assessments by dry combustion technique and MINS demonstrated a linear correlation between the two methods in the 0- to 30-cm soil layer. Based on the developed theoretical model of MINS measurement, we demonstrated that accurate soil carbon determination by this method depends on carbon distribution within the soil and MINS signal errors. C1 [Yakubova, Galina; Kavetskiy, Aleksandr; Torbert, H. Allen; Prior, Stephen A.] USDA ARS, Natl Soil Dynam Lab, Auburn, AL 36832 USA. [Wielopolski, Lucian] Brookhaven Natl Lab, Dept Environm Sci, Upton, NY 11973 USA. RP Yakubova, G (reprint author), USDA ARS, Natl Soil Dynam Lab, 411 South Donahue Dr, Auburn, AL 36832 USA. EM galina.yakubova@ars.usda.gov NR 15 TC 4 Z9 4 U1 2 U2 8 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD DEC PY 2014 VL 179 IS 12 BP 529 EP 535 DI 10.1097/SS.0000000000000099 PG 7 WC Soil Science SC Agriculture GA CE2TL UT WOS:000351671400001 ER PT J AU Goldberg, S AF Goldberg, Sabine TI Chemical Modeling of Boron Adsorption by Humic Materials Using the Constant Capacitance Model SO SOIL SCIENCE LA English DT Article DE Humic acid; B adsorption isotherm; B adsorption envelope ID DISSOLVED ORGANIC-MATTER; ION-BINDING; SOILS; SORPTION; ACID; DESORPTION; EQUILIBRIA; SUBSTANCES AB The constant capacitance surface complexation model was used to describe B adsorption behavior on reference Aldrich humic acid, humic acids from various soil environments, and dissolved organic matter extracted from sewage effluents. The reactive surface functional groups on the humic materials were assumed to be a carboxyl site, XOH, and a phenol site, YOH. Initially, total concentrations of the sites, XOHT and YOHT and the proton dissociation constants for the carboxyl site, LogK(X-), and the phenol site, LogK(Y-), were optimized by fitting the constant capacitance model to potentiometric titration data on reference Aldrich humic acid obtained from the literature. Subsequently, the model was fit to experimental B adsorption data obtained from the literature by optimizing two tetrahedral B surface complexation constants: LogK(XB-) for a carboxyl site and LogK(YB-) for a phenol site. The model was well able to describe the experimental B adsorption data both as a function of solution B concentration (isotherm data) and solution pH (envelope data) for all humic materials. The ability to represent changes in B adsorption as a function of solution pH is the main advantage of the constant capacitance model over adsorption isotherm equations. Results from the current study can be used to describe B adsorption behavior on diverse humic materials of interest in environmental and agricultural situations. C1 USDA ARS, US Salin Lab, Riverside, CA 92507 USA. RP Goldberg, S (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM sabine.goldberg@ars.usda.gov NR 25 TC 0 Z9 0 U1 2 U2 18 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD DEC PY 2014 VL 179 IS 12 BP 561 EP 567 DI 10.1097/SS.0000000000000098 PG 7 WC Soil Science SC Agriculture GA CE2TL UT WOS:000351671400004 ER PT J AU Goldberg, S AF Goldberg, Sabine TI Modeling Selenate Adsorption Behavior on Oxides, Clay Minerals, and Soils Using the Triple Layer Model SO SOIL SCIENCE LA English DT Article DE Selenium; selenite; kaolinite; illite ID CONSTANT CAPACITANCE MODEL; ALUMINA ELECTROLYTE INTERFACE; SURFACE COMPLEXATION MODEL; ELECTRICAL DOUBLE-LAYER; SELENITE ADSORPTION; IRON-OXIDES; VIBRATIONAL SPECTROSCOPY; COMPETITIVE ADSORPTION; SULFATE ADSORPTION; WATER INTERFACE AB Selenate adsorption behavior was investigated on amorphous aluminum oxide; amorphous iron oxide; clay minerals kaolinites, montmorillonites, and illite; and 18 soil samples from Hawaii and the Southwestern and Midwestern regions of the United States as a function of solution pH. Selenate adsorption decreased with increasing solution pH. The triple layer model, a chemical surface complexation model, was able to describe Se (VI) adsorption as a function of solution pH by simultaneously optimizing either two outer-sphere Se(VI) surface complexation constants or one inner-sphere and one outer-sphere Se(VI) surface complexation constant. The fit of the triple layer model to Se(VI) adsorption by oxides, clay minerals, and soils was excellent, as evidenced by very low values of the model variance goodness-of-fit criterion. The predominantly outer-sphere Se(VI) surface speciation predicted using the triple layer model was in agreement with the weak adsorption behavior previously observed for Se(VI) using electrophoretic mobility measurements and ionic strength dependence of adsorption. Direct spectroscopic investigations of Se(VI) surface configurations are needed to corroborate the species predicted by the triple layer modeling approach. C1 USDA ARS, US Salin Lab, Riverside, CA 92507 USA. RP Goldberg, S (reprint author), USDA ARS, US Salin Lab, 450 W Big Springs Rd, Riverside, CA 92507 USA. EM sabine.goldberg@ars.usda.gov NR 68 TC 6 Z9 7 U1 4 U2 27 PU LIPPINCOTT WILLIAMS & WILKINS PI PHILADELPHIA PA TWO COMMERCE SQ, 2001 MARKET ST, PHILADELPHIA, PA 19103 USA SN 0038-075X EI 1538-9243 J9 SOIL SCI JI Soil Sci. PD DEC PY 2014 VL 179 IS 12 BP 568 EP 576 DI 10.1097/SS.0000000000000097 PG 9 WC Soil Science SC Agriculture GA CE2TL UT WOS:000351671400007 ER PT J AU Jusino, MA Lindner, DL Cianchetti, JK Grise, AT Brazee, NJ Walters, JR AF Jusino, Michelle A. Lindner, Daniel L. Cianchetti, John K. Grise, Adam T. Brazee, Nicholas J. Walters, Jeffrey R. TI A minimally invasive method for sampling nest and roost cavities for fungi: a novel approach to identify the fungi associated with cavity-nesting birds SO ACTA ORNITHOLOGICA LA English DT Article DE Basidiomycota specific primer; cavity-excavating birds; cavity-nesting birds; excavators; fungi; hole-nesting birds; ITS4b-21; Picoides borealis; Porodaedalea pini; Red-cockaded Woodpecker; wood decay fungi ID RED-COCKADED WOODPECKER; NORTH-AMERICA; TREES; DECAY; SELECTION; FORESTS AB Relationships among cavity-nesting birds, trees, and wood decay fungi pose interesting management challenges and research questions in many systems. Ornithologists need to understand the relationships between cavity-nesting birds and fungi in order to understand the habitat requirements of these birds. Typically, researchers rely on fruiting body surveys to identify the fungal players in these relationships. Fruiting body surveys enable nondestructive sampling, but vastly underestimate fungal presence and diversity and may miss species of critical importance to cavity- nesting birds; thus new methods for such analyses are necessary. Here we present a novel technique to nondestructively sample the wood inside tree cavities, which produces samples that can be processed using DNA-based methods to identify fungi. We tested our method on Red-cockaded Woodpecker Picoides borealis excavations, half of which were from trees with Porodaedalea pini fruiting bodies. Using our new approach, we detected P. pini in 90% of the excavations in trees with fruiting bodies, but also in 60% of the excavations in trees without fruiting bodies and identified nine additional taxa of wood decay fungi that did not have fruiting bodies present. Our approach offers improved detection of fungi through non-destructive sampling of excavated cavities and we developed an improved primer specific to the fungal phylum that contains most wood decay fungi (Basidiomycota), thus providing managers and researchers a critical tool to better determine which fungi are important to cavity-nesting birds. C1 [Jusino, Michelle A.] Univ Wisconsin, Dept Med Microbiol & Immunol, Madison, WI 53706 USA. [Jusino, Michelle A.; Walters, Jeffrey R.] Virginia Tech, Dept Biol Sci, Blacksburg, VA 24061 USA. [Lindner, Daniel L.] US Forest Serv, Ctr Forest Mycol Res, No Res Stn, Madison, WI 53726 USA. [Cianchetti, John K.] Cornell Univ, Dept Entomol, Ithaca, NY 14850 USA. [Grise, Adam T.] Cultural Acad Excellence, Hyattsville, MD 20782 USA. [Brazee, Nicholas J.] Univ Massachusetts, Ctr Agr, UMass Extens, Amherst, MA 01002 USA. RP Jusino, MA (reprint author), Univ Wisconsin, Dept Med Microbiol & Immunol, 1550 Linden Dr, Madison, WI 53706 USA. EM mjusino@wisc.edu FU U.S. Department of Defense, Marine Corps Base Camp Lejeune; Harold H. Bailey fund at Virginia Tech; U.S. Department of Defense, Strategic Environmental Research and Development Program [RC-1471, RC-1413] FX We sincerely thank Mark T. Banik for help with primer design, Kevin R. Rose for help in the field, Dana M. Hawley, Robert H. Jones and David G. Schmale III for guidance, and the Virginia Tech Avian Ecology Lab, James Skelton, Jim Bednarz, and one anonymous reviewer for helpful comments on this manuscript. We also acknowledge Uri Geller for his outstanding previous work with (usually unsharpened) spoons. Funding for fieldwork was provided by the U.S. Department of Defense, Marine Corps Base Camp Lejeune, and funding for analysis of samples was provided by the Harold H. Bailey fund at Virginia Tech. Further support for MAJ was provided by the U.S. Department of Defense, Strategic Environmental Research and Development Program (RC-1471 and RC-1413, Defense Coastal/Estuarine Research Program). Laboratory space was provided by the U.S. Forest Service, Northern Research Station, Center for Forest Mycology Research. NR 28 TC 3 Z9 3 U1 3 U2 7 PU MUSEUM & INST ZOOLOGY PI WARSAW PA WILCZA 64, PL-00-679 WARSAW, POLAND SN 0001-6454 EI 1734-8471 J9 ACTA ORNITHOL JI Acta Ornithol. PD DEC PY 2014 VL 49 IS 2 BP 233 EP 242 DI 10.3161/173484714X687127 PG 10 WC Ornithology SC Zoology GA CD3FR UT WOS:000350963900009 ER PT J AU Liang, Y Payne, JB Penn, C Tabler, GT Watkins, SE VanDevender, KW Purswell, JL AF Liang, Y. Payne, J. B. Penn, C. Tabler, G. T. Watkins, S. E. VanDevender, K. W. Purswell, J. L. TI Systematic evaluation of in-house broiler litter windrowing effects on production benefits and environmental impact SO JOURNAL OF APPLIED POULTRY RESEARCH LA English DT Article DE windrowing; Sodium Hydroxide; bacteria; litter; ammonia; temperature ID POULTRY LITTER; BIOSECURITY; FARM; SOIL AB In-house windrowing of broiler litter between flocks has been adopted by producers to reduce pathogens and improve litter quality before chick placement. In this study, 5 consecutive windrow trials were conducted in commercial broiler houses for their effect on litter bacterial populations, organic matter stabilization, cumulative ammonia emissions, and nutrient transformation and compared with litter conditioning (tilling) in adjacent houses. No significant reduction of Clostridium spp. and Escherichia coli populations was found in windrowed litter from d 0 to 7. No significant difference of 7-d mortality was found between windrow and nonwindrow houses. The windrowed house resulted in better foot quality than the nonwindrowed house from 1 of 3 scored flocks. Water-soluble phosphorus increased in both windrowed and nonwindrowed litter; therefore, appreciable biotic and abiotic activity occurred in litter with both treatments after flocks were removed. Overall, no negative effect of windrow treatments on litter quality for agronomic applications was observed. Both the control and windrow treatments resulted in a decrease in litter moisture content (2 to 5%) likely beneficial to bird health conditions. High ammonia emissions persisted after windrow spreading; therefore, a need may exist for an extended period of ventilation or a litter amendment as crucial before chick placement. Litter amendment at a low dose was effective in lowering ammonia concentrations after windrowing and was more economical comparing to operating fans in winter conditions. C1 [Liang, Y.; VanDevender, K. W.] Univ Arkansas, Dept Biol & Agr Engn, Fayetteville, AR 72701 USA. [Payne, J. B.] Oklahoma State Univ, Dept Biosyst & Agr Engn, Stillwater, OK 74074 USA. [Penn, C.] Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74074 USA. [Tabler, G. T.] Mississippi State Univ, Extens Serv, Dept Poultry Sci, Mississippi State, MS 39762 USA. [Watkins, S. E.] Univ Arkansas, Ctr Excellence Poultry Sci, Fayetteville, AR 72701 USA. [Purswell, J. L.] USDA Agr Res Serv, Poultry Res Unit, Mississippi State, MS 39762 USA. RP Liang, Y (reprint author), Univ Arkansas, Dept Biol & Agr Engn, Fayetteville, AR 72701 USA. EM yliang@uark.edu FU United States Poultry and Egg Association; University of Arkansas Division of Agriculture FX Funding for this project was provided by the United States Poultry and Egg Association (Tucker, GA) and the University of Arkansas Division of Agriculture (Little Rock). The authors thank Chance Williams and David McCreery (University of Arkansas, Fayetteville) for their assistance with the experiments. NR 27 TC 1 Z9 1 U1 1 U2 5 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 1056-6171 EI 1537-0437 J9 J APPL POULTRY RES JI J. Appl. Poult. Res. PD DEC PY 2014 VL 23 IS 4 BP 625 EP 638 DI 10.3382/japr.2014-00960 PG 14 WC Agriculture, Dairy & Animal Science SC Agriculture GA AU4EX UT WOS:000345563600006 ER PT J AU Lorusso, A Ciacci-Zanella, JR Zanella, EL Pena, L Perez, DR Lager, KM Rajao, DS Loving, CL Kitikoon, P Vincent, AL AF Lorusso, Alessio Ciacci-Zanella, Janice R. Zanella, Eraldo L. Pena, Lindomar Perez, Daniel R. Lager, Kelly M. Rajao, Daniela S. Loving, Crystal L. Kitikoon, Pravina Vincent, Amy L. TI Polymorphisms in the haemagglutinin gene influenced the viral shedding of pandemic 2009 influenza virus in swine SO JOURNAL OF GENERAL VIROLOGY LA English DT Article ID UNITED-STATES; A VIRUSES; BINDING; RECEPTORS; HUMANS; AIRWAY; PIGS AB Interactions between the viral surface glycoprotein haemagglutinin (HA) and the corresponding receptors on host cells is one important aspect of influenza virus infection. Mutations in HA have been described to affect pathogenicity, antigenicity and the transmission of influenza viruses. Here, we detected polymorphisms present in HA genes of two pandemic 2009 H1N1 (H1N1pdm09) isolates, A/California/04/2009 (Ca/09) and A/Mexico/4108/2009 (Mx/09), that resulted in amino acid changes at positions 186 (S to P) and 194 (L to I) of the mature HA1 protein. Although not reported in the published H1N1pdm09 consensus sequence, the P186 genotype was more readily detected in primary infected and contact-naive pigs when inoculated with a heterogeneous mixed stock of Ca/09. Using reverse genetics, we engineered Ca/09 and Mx/09 genomes by introducing Ca/09 HA with two naturally occurring variants expressing S186/1194 (HA-S/I) and P186/L194 (HA-P/L), respectively. The Ca/09 HA with the combination of P186/L194 with either the Ca/09 or Mx/09 backbone resulted in higher and prolonged viral shedding in naive pigs. This efficiency appeared to be more likely through an advantage in cell surface attachment rather than replication efficiency. Although these mutations occurred within the receptor-binding pocket and the Sb antigenic site, they did not affect serological cross-reactivity. Relative increases of P186 in publicly available sequences from swine H1N1pdm09 viruses supported the experimental data, indicating this amino acid substitution conferred an advantage in swine. C1 [Lorusso, Alessio; Ciacci-Zanella, Janice R.; Zanella, Eraldo L.; Lager, Kelly M.; Rajao, Daniela S.; Loving, Crystal L.; Kitikoon, Pravina; Vincent, Amy L.] USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. [Ciacci-Zanella, Janice R.] Embrapa Suinos & Ayes, Virol Lab, Concordia, SC, Brazil. [Zanella, Eraldo L.] Univ Passo Fundo, Casca, RS, Brazil. [Pena, Lindomar; Perez, Daniel R.] Univ Maryland, Dept Vet Med, College Pk, MD 20742 USA. [Pena, Lindomar; Perez, Daniel R.] Univ Maryland, Virginia Maryland Reg Coll Vet Med, College Pk, MD 20742 USA. RP Vincent, AL (reprint author), USDA ARS, Virus & Prion Res Unit, Natl Anim Dis Ctr, Ames, IA 50011 USA. EM amy.vincent@ars.usda.gov RI Lorusso, Alessio/A-7311-2016; Zanella, Janice/C-3632-2014; OI Lorusso, Alessio/0000-0001-7933-7367; Perez, Daniel/0000-0002-6569-5689; de Souza Rajao, Daniela/0000-0002-0772-0065; Lorusso, Alessio/0000-0001-6156-8212 FU USDA-ARS; DHHS-CDC FX The authors thank Michelle Harland and Hillary Horst for technical assistance; David Alt and Lea Ann Hobbs for sequencing; and Dr Becky Jepsen, Brian Pottebaum, Jason Huegel and Jason Crabtree for assistance with animal studies. Isolates of H1N1pdm09 were generously provided by the late Dr Alexander Klimov (Centers for Disease Control and Prevention). Mention of trade names or commercial products in this paper is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture (USDA). Funding was provided by USDA-ARS and DHHS-CDC. USDA is an equal opportunity provider and employer. NR 19 TC 1 Z9 1 U1 1 U2 8 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 0022-1317 EI 1465-2099 J9 J GEN VIROL JI J. Gen. Virol. PD DEC PY 2014 VL 95 BP 2618 EP 2626 DI 10.1099/vir.0.067926-0 PN 12 PG 9 WC Biotechnology & Applied Microbiology; Virology SC Biotechnology & Applied Microbiology; Virology GA CD2YB UT WOS:000350943800004 PM 25127710 ER PT J AU Prince, AM Khan, FH Matthews, BF Islam, N Natarajan, SS AF Prince, A. M. Khan, F. H. Matthews, B. F. Islam, N. Natarajan, S. S. TI TRANSCRIPTOME AND PROTEOME ALTERATIONS DURING THE NEMATODE-SOYBEAN INTERACTION SO NEMATROPICA LA English DT Review DE Heterodera glycines; Meloidogyne incognita; nematodes; protein; soybean ID LASER CAPTURE MICRODISSECTION; ESOPHAGEAL GLAND-CELLS; ROOT-KNOT NEMATODES; MAX GENOTYPE PEKING; HETERODERA-GLYCINES; CYST-NEMATODE; GENE-EXPRESSION; MICROARRAY ANALYSIS; RESISTANT REACTION; FEEDING CELLS AB Plant-parasitic nematodes are important pests that cause an estimated worldwide loss of $100 billion annually. The root-knot and cyst nematodes are of major economic importance to soybean. Consequently, the need for comprehensive understanding of these nematodes directs researchers to combine the transcriptome and proteome analyses to determine the interplay between alterations in soybean gene expression and nematode parasitism. Microarray studies have found several thousand alterations in transcript abundance during nematode parasitism; proteomics has allowed analysis of thousands of root and nematode proteins. The current knowledge of root-nematode interactions is mostly based on transcriptomic data with few proteomic studies. The root-knot and soybean cyst nematode proteins are the topic of this review because they play an important role in manipulation of soybean root cell biology. A better understanding of these proteins and the altered soybean proteins in response to nematode parasitism would help in the discovery of new nematode resistance targets. C1 [Prince, A. M.; Khan, F. H.; Matthews, B. F.; Natarajan, S. S.] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. [Prince, A. M.] Cairo Univ, Fac Vet Med, Dept Biochem, Giza, Egypt. [Islam, N.] Univ Maryland, Dept Plant Sci & Landscape Architecture, College Pk, MD 20742 USA. RP Natarajan, SS (reprint author), USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD 20705 USA. EM savi.natarajan@ars.usda.gov FU United Soybean Board project [Y9254] FX The authors gratefully acknowledge support from a grant from United Soybean Board project number Y9254. NR 63 TC 0 Z9 0 U1 3 U2 18 PU ORGANIZATION TROP AMER NEMATOLOGISTS PI AUBURN PA AUBURN UNIV DEPT PLANT PATHOLOGY, AUBURN, AL 36849 USA SN 0099-5444 EI 2220-5608 J9 NEMATROPICA JI Nematropica PD DEC PY 2014 VL 44 IS 2 BP 137 EP 145 PG 9 WC Zoology SC Zoology GA CC6BQ UT WOS:000350450000003 ER PT J AU Webber, CL Taylor, MJ Shrefler, JW AF Webber, Charles L., III Taylor, Merritt J. Shrefler, James W. TI Weed Control in Sweet Bell Pepper Using Sequential Postdirected Applications of Pelargonic Acid SO HORTTECHNOLOGY LA English DT Article DE Amaranthus spinosus; Capsicum annuum; crop injury; crop yield; cutleaf groundcherry; Cyperus esculentus; Digitaria ischaemum; Physalis angulata; phytotoxicity; smooth crabgrass; spiny amaranth; yellow nutsedge AB Pepper (Capsicum annuum) producers would benefit from additional herbicide options that are safe to the crop and provide effective weed control. Research was conducted in southeastern Oklahoma (Atoka County, Lane, OK) during 2010 and 2011 to determine the impact of pelargonic acid on weed control efficacy, crop injury, and pepper yields. The experiment included pelargonic acid applied unshielded postdirected at 5, 10, and 15 lb/acre, plus an untreated weedy control and an untreated weed-free control. 'Jupiter' sweet bell pepper, a tobacco mosaic virus-resistant sweet pepper with a 70-day maturity, was transplanted into single rows on 3-ft centered raised beds with 18 inches between plants (9680 plants/acre) on 28 May 2010 and 27 May 2011, respectively. Weeds included smooth crabgrass (Digitaria ischaemum), cutleaf groundcherry (Physalis angulata), spiny amaranth (Amaranthus spinosus), and yellow nutsedge (Cyperus esculentus). Pelargonic acid was applied postdirected each year in mid-June and then reapplied 8 days later. The 15-lb/acre pelargonic acid treatment resulted in the maximum smooth crabgrass control (56%) and broadleaf weed control (66%) at 1 day after the initial spray treatment (DAIT), and 33% yellow nutsedge control at 3 DAIT. Pelargonic acid at 15 lb/acre provided equal or slightly greater smooth crabgrass and broadleaf (cutleaf groundcherry and spiny amaranth) control compared with the 10-lb/acre application, and consistently greater control than the 5-lb/acre rate and the weedy control. Pelargonic acid was less effective at controlling yellow nutsedge than smooth crabgrass and broadleaf weeds. As the rate of pelargonic acid increased from 5 to 15 lb/acre, yellow nutsedge control also increased significantly for all observation dates. Increasing the pelargonic acid application rate increased the crop injury rating. The maximum crop injury occurred for each application rate at 1 DAIT with 7%, 8.0%, and 13.8% injury for pelargonic acid rates 5, 10, and 15 lb/acre, respectively. There was little or no new crop injury after the second postdirected application of pelargonic acid and crop injury following 3 DAIT for application rates was 2% or less. Only the 15-lb/acre pelargonic acid application produced greater fruit per hectare (4784 fruit/ha) and yields (58.65 kg.ha(-1)) than the weedy control (1196 fruit/ha and 19.59 kg.ha(-1)). The weed-free yields (7176 fruit/ha, 178.11 kg.ha(-1), and 24.82 g/fruit) were significantly greater than all pelargonic acid treatments and the weedy control. Pelargonic acid provided unsatisfactory weed control for all rates and did not significantly benefit from the sequential applications. The authors suggest the pelargonic acid be applied to smaller weeds to increase the weed control to acceptable levels (>80%). C1 [Webber, Charles L., III] USDA, Agr Res Serv, Sugarcane Res Unit, Houma, LA 70360 USA. [Taylor, Merritt J.; Shrefler, James W.] Oklahoma State Univ, Cooperat Extens Serv, Div Agr Sci & Nat Resources, Durant, OK 74702 USA. RP Webber, CL (reprint author), USDA, Agr Res Serv, Sugarcane Res Unit, Houma, LA 70360 USA. EM Webber@ars.usda.gov NR 11 TC 1 Z9 1 U1 5 U2 6 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD DEC PY 2014 VL 24 IS 6 BP 663 EP 667 PG 5 WC Horticulture SC Agriculture GA CB6MQ UT WOS:000349741500005 ER PT J AU Johnson, WC Davis, JW AF Johnson, Wiley Carroll, III Davis, Jerry W. TI Pelargonic Acid for Weed Control in Organic Vidalia (R) Sweet Onion Production SO HORTTECHNOLOGY LA English DT Article DE Allium cepa; dry-bulb onion; organic weed control ID CLOVE OIL HERBICIDE; VINEGAR; CORN AB Cultivation using a tine weeder is a proven means to manage weeds in organic Vidalia sweet onion (Allium cepa) production. If the initial cultivation is delayed, emerged weeds are not controlled. In these cases, herbicides derived from natural products could be used to control the emerged weeds before the initial cultivation. Clove oil has been evaluated for this use, but cool-season weed control is inconsistent during the winter season when Vidalia sweet onion are grown. Pelargonic acid is a herbicide that can be derived from natural sources or synthesized. Field trials were conducted from 2011 through 2013 to determine the efficacy of pelargonic acid for cool-season weed control in organic Vidalia sweet onion. All possible combinations of four herbicides and three cultivation regimes using a tine weeder were evaluated. Herbicides evaluated were pelargonic acid (3% and 5% by vol.), clove oil [10% by vol. (2011 and 2012)], D-limonene [14% (2013 only)], and a nontreated control. Cultivation regimes were twice (2x) and four times (4x) at 2-week intervals, and a noncultivated control. Main effects of cultivation and herbicides were independent for all parameters, with no improvement when used in combination. Cultivation 2x and 4x controlled cool-season weeds and improved onion yields, which is consistent with previous research. Pelargonic acid (5%) controlled weeds similar to clove oil (2011 and 2012) and D-limonene (2013), with cool-season weed control efficacy being inconsistent among all herbicides. Onion yield response to weed control from any of the herbicides, including pelargonic acid, also was inconsistent. In organic onion production, inconsistent cool-season performance using pelargonic acid is similar to other herbicides derived from natural sources. C1 [Johnson, Wiley Carroll, III] USDA ARS, Tifton, GA 31793 USA. [Davis, Jerry W.] Univ Georgia, Griffin, GA 30223 USA. RP Johnson, WC (reprint author), USDA ARS, Tifton Campus,POB 748, Tifton, GA 31793 USA. EM Carroll.johnson@ars.usda.gov FU Vidalia Onion and Vegetable Research Center FX We acknowledge the technical contributions of Daniel R. Evarts whose skills made these studies possible. Appreciation is expressed to C. Randy Hill and Denny R. Thigpen at the Vidalia Onion and Vegetable Research Center for their support of this research study. We appreciate the managerial support and assistance rendered by Stephen Powell, Corrections Officer - II, Rodgers State Prison, Reidsville, GA. NR 23 TC 0 Z9 0 U1 4 U2 8 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 1063-0198 EI 1943-7714 J9 HORTTECHNOLOGY JI HortTechnology PD DEC PY 2014 VL 24 IS 6 BP 696 EP 701 PG 6 WC Horticulture SC Agriculture GA CB6MQ UT WOS:000349741500009 ER PT J AU Urban, LE Roberts, SB Fierstein, JL Gary, CE Lichtenstein, AH AF Urban, Lorien E. Roberts, Susan B. Fierstein, Jamie L. Gary, Christine E. Lichtenstein, Alice H. TI Sodium, Saturated Fat, and Trans Fat Content Per 1,000 Kilocalories: Temporal Trends in Fast-Food Restaurants, United States, 2000-2013 SO PREVENTING CHRONIC DISEASE LA English DT Article ID CARDIOVASCULAR-DISEASE; US ADULTS; REDUCTION; PURCHASES; BURDEN AB Introduction Intakes of sodium, saturated fat, and trans fat remain high despite recommendations to limit these nutrients for cardiometabolic risk reduction. A major contributor to intake of these nutrients is foods prepared outside the home, particularly from fast-food restaurants. Methods We analyzed the nutrient content of frequently ordered items from 3 US national fast-food chains: fried potatoes (large French fries), cheeseburgers (2-oz and 4-oz), and a grilled chicken sandwich. We used an archival website to obtain data on sodium, saturated fat, and trans fat content for these items from 2000 through 2013. The amount of each nutrient per 1,000 kcal was calculated to determine whether there were trends in product reformulation. Results Sodium content per 1,000 kcal differed widely among the 3 chains by food item, precluding generalizations across chains. During the 14-year period, sodium content per 1,000 kcal for large French fries remained high for all 3 chains, although the range narrowed from 316-2,000 mg per 1,000 kcal in 2000 to 700-1,420 mg per 1,000 kcal in 2013. Among the items assessed, cheeseburgers were the main contributor of saturated fat, and there was little change in content per 1,000 kcal for this item during the 14-year period. In contrast, there was a sharp decline in saturated and trans fat content of large French fries per 1,000 kcal. Post-2009, the major contributor of trans fat per 1,000 kcal was cheeseburgers; trans fat content of this item remained stable during the 14-year period. Conclusion With the exception of French fries, little evidence was found during the 14-year period of product reformulation by restaurants to become more consistent with dietary guidance to reduce intakes of sodium and saturated fat. C1 [Urban, Lorien E.; Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fierstein, Jamie L.; Gary, Christine E.] Tufts Univ, Freidman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM alice.lichtenstein@tufts.edu FU US Department of Agriculture [58-1950-0-0014, 1950-51,000-072-02S]; Tufts University FX This work was supported by the US Department of Agriculture under agreement nos. 58-1950-0-0014 and 1950-51,000-072-02S with Tufts University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the US Department of Agriculture. The authors thank Ashley Equi for her help with data acquisition. NR 19 TC 3 Z9 3 U1 4 U2 11 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD DEC PY 2014 VL 11 AR E228 DI 10.5888/pcd11.140335 PG 5 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CB8BH UT WOS:000349852700012 PM 25551183 ER PT J AU Urban, LE Roberts, SB Fierstein, JL Gary, CE Lichtenstein, AH AF Urban, Lorien E. Roberts, Susan B. Fierstein, Jamie L. Gary, Christine E. Lichtenstein, Alice H. TI Temporal Trends in Fast-Food Restaurant Energy, Sodium, Saturated Fat, and Trans Fat Content, United States, 1996-2013 SO PREVENTING CHRONIC DISEASE LA English DT Article ID WEIGHT-GAIN; RISK; CONSUMPTION; PURCHASES; NUTRITION; BURDEN; ADULTS; STYLE; WOMEN; MEN AB Introduction Excess intakes of energy, sodium, saturated fat, and trans fat are associated with increased risk for cardiometabolic syndrome. Trends in fast-food restaurant portion sizes can inform policy decisions. We examined the variability of popular food items in 3 fast-food restaurants in the United States by portion size during the past 18 years. Methods Items from 3 national fast-food chains were selected: French fries, cheeseburgers, grilled chicken sandwich, and regular cola. Data on energy, sodium, saturated fat, and trans fat content were collated from 1996 through 2013 using an archival website. Time trends were assessed using simple linear regression models, using energy or a nutrient component as the dependent variable and the year as the independent variable. Results For most items, energy content per serving differed among chain restaurants for all menu items (P <= .04); energy content of 56% of items decreased (beta range, -0.1 to -5.8 kcal) and the content of 44% increased (beta range, 0.6-10.6 kcal). For sodium, the content of 18% of the items significantly decreased (beta range, -4.1 to -24.0 mg) and the content for 33% increased (beta range, 1.9-29.6 mg). Absolute differences were modest. The saturated and trans fat content, post-2009, was modest for French fries. In 2013, the energy content of a large-sized bundled meal (cheeseburger, French fries, and regular cola) represented 65% to 80% of a 2,000-calorie-perday diet, and sodium content represented 63% to 91% of the 2,300-mg-per-day recommendation and 97% to 139% of the 1,500-mg-per-day recommendation. Conclusion Findings suggest that efforts to promote reductions in energy, sodium, saturated fat, and trans fat intakes need to be shifted from emphasizing portion-size labels to additional factors such as total calories, frequency of eating, number of items ordered, menu choices, and energy-containing beverages. C1 [Urban, Lorien E.; Roberts, Susan B.] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 USA. [Fierstein, Jamie L.; Gary, Christine E.] Tufts Univ, Freidman Sch Nutr Sci & Policy, Boston, MA 02111 USA. RP Lichtenstein, AH (reprint author), Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Cardiovasc Nutr Lab, 711 Washington St, Boston, MA 02111 USA. EM alice.lichtenstein@tufts.edu FU US Department of Agriculture [58-1950-0-0014, 1950-51000-072-02S]; Tufts University FX This work was supported by the US Department of Agriculture under agreement nos. 58-1950-0-0014 and 1950-51000-072-02S with Tufts University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the authors and do not necessarily reflect the views of the US Department of Agriculture. The authors thank Ashley Equi for her help with data acquisition. NR 28 TC 0 Z9 0 U1 0 U2 14 PU CENTERS DISEASE CONTROL PI ATLANTA PA 1600 CLIFTON RD, ATLANTA, GA 30333 USA SN 1545-1151 J9 PREV CHRONIC DIS JI Prev. Chronic Dis. PD DEC PY 2014 VL 11 AR E229 DI 10.5888/pcd11.140202 PG 7 WC Public, Environmental & Occupational Health SC Public, Environmental & Occupational Health GA CB8BH UT WOS:000349852700005 PM 25551184 ER PT J AU Silva, CA Klauberg, C Carvalho, SDCE Hudak, AT Rodriguez, LCE AF Silva, Carlos Alberto Klauberg, Carine Chaves e Carvalho, Samuel de Padua Hudak, Andrew T. Estraviz Rodriguez, Luiz Carlos TI Mapping aboveground carbon stocks using LiDAR data in Eucalyptus spp. plantations in the state of Sao Paulo, Brazil SO SCIENTIA FORESTALIS LA English DT Article DE Airborne Laser Scanning ALS; LiDAR metrics; C stock; fast growing plantation ID AIRBORNE LASER; FOREST; PARAMETERS; REGRESSION; INTENSITY; STAND AB Fast growing plantation forests provide a low-cost means to sequester carbon for greenhouse gas abatement. The aim of this study was to evaluate airborne LiDAR (Light Detection And Ranging) to predict aboveground carbon (AGC) stocks in Eucalyptus spp. plantations. Biometric parameters (tree height (Ht) and diameter at breast height (DBH)) were collected from conventional forest inventory sample plots. Regression models predicting total aboveground carbon (AGCt), aboveground carbon in commercial logs (AGCc), and aboveground carbon in harvest residuals (AGCr) from LiDAR-derived canopy structure metrics were developed and evaluated for predictive power and parsimony. The best models from a family of six models were selected based on corrected Akaike Information Criterion (AlCc) and assessed by the root mean square error (RMSE) and coefficient of determination (R-2-adj). The best three models to estimate AGC stocks were AGCt: R-2-adj = 0.81, RMSE = 7.70 Mg.ha(-1); AGCc: R-2-adj = 0.83, RMSE = 5.26 Mg.ha(-1); AGCr: R-2-adj = 0.71, RMSE = 2.67 Mg.ha(-1). This study showed that LiDAR canopy structure metrics can be used to predict AGC stocks in Eucalyptus spp. plantations in Brazil with high accuracy. We conclude that there is good potential to monitor growth and carbon sequestration in Eucalyptus spp. plantations using LiDAR. C1 [Silva, Carlos Alberto; Estraviz Rodriguez, Luiz Carlos] ESALQ USP, Dept Forest Sci, BR-13418900 Piracicaba, SP, Brazil. [Klauberg, Carine] US Forest Serv, USDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. [Chaves e Carvalho, Samuel de Padua] Univ Fed Mato Grosso, Dept Forest Sci, BR-78060900 Cuiaba, MT, Brazil. [Hudak, Andrew T.] US Forest Serv, ISDA, Rocky Mt Res Stn, Moscow, ID 83843 USA. RP Silva, CA (reprint author), ESALQ USP, Dept Forest Sci, Av Padua Dias 11, BR-13418900 Piracicaba, SP, Brazil. EM carlos_engflorestal@outlook.com; carine_klauberg@hotmail.com; sam.padua@gmail.com; ahudak@fs.fed.us; lcer@usp.br RI CARVALHO, SAMUEL/F-8339-2014; Rodriguez, Luiz Carlos/D-7043-2012; Silva, Carlos Alberto/A-8112-2015 OI CARVALHO, SAMUEL/0000-0002-5590-9049; Rodriguez, Luiz Carlos/0000-0002-1430-7981; FU US Forest Service (USFS) Rocky Mountain Research Station at the Forestry Sciences Laboratory in Moscow, Idaho; USFS International Programs; FAPESP [2012/03176-0]; FIBRIA, a Brazilian pulp and paper company FX We thank the US Forest Service (USFS) Rocky Mountain Research Station at the Forestry Sciences Laboratory in Moscow, Idaho and USFS International Programs for their support. This study was also supported by FAPESP (Process no 2012/03176-0 MSc assistantship). LiDAR data collections were funded by FIBRIA, a Brazilian pulp and paper company. NR 36 TC 5 Z9 5 U1 1 U2 10 PU IPEF-INST PESQUISAS ESTUDOS FLORESTAIS PI PIRACICABA PA PADUA DIAS AVE 11, PO BOX 530, PIRACICABA, SP 13400-970, BRAZIL SN 1413-9324 J9 SCI FOR JI Sci. For. PD DEC PY 2014 VL 42 IS 104 BP 591 EP 604 PG 14 WC Forestry SC Forestry GA CC2MF UT WOS:000350178400013 ER PT J AU Lockeand, AM Ort, DR AF Lockeand, Anna M. Ort, Donald R. TI Leaf hydraulic conductance declines in coordination with photosynthesis, transpiration and leaf water status as soybean leaves age regardless of soil moisture SO JOURNAL OF EXPERIMENTAL BOTANY LA English DT Article DE Development; drought; leaf age; leaf hydraulic conductance; leaf water potential; photosynthesis; senescence; stomatal conductance ID ELEVATED CARBON-DIOXIDE; AIR CO2 ENRICHMENT; LONG-TERM GROWTH; STOMATAL CONDUCTANCE; XYLEM CAVITATION; USE EFFICIENCY; DROUGHT STRESS; SEASONAL-CHANGES; EMBOLISM; TREES AB Photosynthesis requires sufficient water transport through leaves for stomata to remain open as water transpires from the leaf, allowing CO2 to diffuse into the leaf. The leaf water needs of soybean change over time because of large microenvironment changes over their lifespan, as leaves mature in full sun at the top of the canopy and then become progressively shaded by younger leaves developing above. Leaf hydraulic conductance (K-leaf), a measure of the leaf's water transport capacity, can often be linked to changes in microenvironment and transpiration demand. In this study, we tested the hypothesis that K-leaf would decline in coordination with transpiration demand as soybean leaves matured and aged. Photosynthesis (A), stomatal conductance (g(s)) and leaf water potential (Psi(leaf)) were also measured at various leaf ages with both field- and chamber-grown soybeans to assess transpiration demand. K-leaf was found to decrease as soybean leaves aged from maturity to shading to senescence, and this decrease was strongly correlated with midday A. Decreases in K-leaf were further correlated with decreases in gs, although the relationship was not as strong as that with A. Separate experiments investigating the response of K-leaf to drought demonstrated no acclimation of K-leaf to drought conditions to protect against cavitation or loss of gs during drought and confirmed the effect of leaf age in K-leaf observed in the field. These results suggest that the decline of leaf hydraulic conductance as leaves age keeps hydraulic supply in balance with demand without K-leaf becoming limiting to transpiration water flux. C1 [Lockeand, Anna M.; Ort, Donald R.] Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. [Ort, Donald R.] Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA. [Ort, Donald R.] ARS, Global Change & Photosynth Res Unit, USDA, Urbana, IL 61801 USA. RP Ort, DR (reprint author), Univ Illinois, Dept Plant Biol, Urbana, IL 61801 USA. EM d-ort@igb.illinois.edu FU National Research Initiative of Agriculture and Food Research Initiative Competitive Grants Program [2010-65114-20343] FX This project was supported in part by the National Research Initiative of Agriculture and Food Research Initiative Competitive Grants Program (2010-65114-20343). NR 71 TC 9 Z9 9 U1 6 U2 50 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0022-0957 EI 1460-2431 J9 J EXP BOT JI J. Exp. Bot. PD DEC PY 2014 VL 65 IS 22 BP 6617 EP 6627 DI 10.1093/jxb/eru380 PG 11 WC Plant Sciences SC Plant Sciences GA AZ3WJ UT WOS:000348154300023 PM 25281701 ER PT J AU Rothrock, MJ Hiett, KL Gamble, J Caudill, AC Cicconi-Hogan, KM Caporaso, JG AF Rothrock, Michael J., Jr. Hiett, Kelli L. Gamble, John Caudill, Andrew C. Cicconi-Hogan, Kellie M. Caporaso, J. Gregory TI A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Molecular Biology; Issue 94; DNA extraction; poultry; environmental; feces; litter; semi-automated; microbiomics; qPCR ID HUMAN FECAL SAMPLES; MICROBIAL COMMUNITY; LITTER; SEQUENCES; QIIME; KITS AB The efficacy of DNA extraction protocols can be highly dependent upon both the type of sample being investigated and the types of downstream analyses performed. Considering that the use of new bacterial community analysis techniques (e.g., microbiomics, metagenomics) is becoming more prevalent in the agricultural and environmental sciences and many environmental samples within these disciplines can be physiochemically and microbiologically unique (e.g., fecal and litter/bedding samples from the poultry production spectrum), appropriate and effective DNA extraction methods need to be carefully chosen. Therefore, a novel semi-automated hybrid DNA extraction method was developed specifically for use with environmental poultry production samples. This method is a combination of the two major types of DNA extraction: mechanical and enzymatic. A two-step intense mechanical homogenization step (using bead-beating specifically formulated for environmental samples) was added to the beginning of the "gold standard" enzymatic DNA extraction method for fecal samples to enhance the removal of bacteria and DNA from the sample matrix and improve the recovery of Gram-positive bacterial community members. Once the enzymatic extraction portion of the hybrid method was initiated, the remaining purification process was automated using a robotic workstation to increase sample throughput and decrease sample processing error. In comparison to the strict mechanical and enzymatic DNA extraction methods, this novel hybrid method provided the best overall combined performance when considering quantitative (using 16S rRNA qPCR) and qualitative (using microbiomics) estimates of the total bacterial communities when processing poultry feces and litter samples. C1 [Rothrock, Michael J., Jr.; Cicconi-Hogan, Kellie M.] ARS, Egg Safety & Qual Res Unit, USDA, Washington, DC 20250 USA. [Hiett, Kelli L.] ARS, Poultry Microbiol Safety & Proc Res Unit, USDA, Washington, DC 20250 USA. [Gamble, John] Oregon State Univ, Dept Biochem & Biophys, Corvallis, OR 97331 USA. [Caudill, Andrew C.] Univ Georgia, Coll Publ Hlth, Athens, GA 30602 USA. [Caporaso, J. Gregory] No Arizona Univ, Dept Biol Sci, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA. RP Rothrock, MJ (reprint author), ARS, Egg Safety & Qual Res Unit, USDA, Washington, DC 20250 USA. EM mjrothrock@gmail.com FU Agricultural Research Service, USDA CRIS [6612-41420-017-00, 6612-32000-059-00] FX The authors would like to acknowledge Latoya Wiggins and Katelyn Griffin for their assistance in sample acquisition, as well as Laura Lee Rutherford for their assistance in sampling and molecular analyses. We would also like to thank Sarah Owens from Argonne National Lab for microbiomic sample preparation and sequencing. These investigations were supported equally by the Agricultural Research Service, USDA CRIS Projects "Pathogen Reduction and Processing Parameters in Poultry Processing Systems" #6612-41420-017-00 and "Molecular Approaches for the Characterization of Foodborne Pathogens in Poultry" #6612-32000-059-00. NR 33 TC 0 Z9 0 U1 2 U2 13 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD DEC PY 2014 IS 94 AR e52161 DI 10.3791/52161 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB1AE UT WOS:000349358000038 ER PT J AU Sparks, JT Dickens, JC AF Sparks, Jackson T. Dickens, Joseph C. TI Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti SO JOVE-JOURNAL OF VISUALIZED EXPERIMENTS LA English DT Article DE Molecular Biology; Issue 94; Gustation; insect; Aedes aegypti; electrophysiology; mosquito; RNA-seq; qRT-PCR; taste; chemosensory ID INSECT REPELLENTS; DEET; SEQ; EXPRESSION; RECEPTORS; FAMILY AB Electrophysiological recording of action potentials from sensory neurons of mosquitoes provides investigators a glimpse into the chemical perception of these disease vectors. We have recently identified a bitter sensing neuron in the labellum of female Aedes aegypti that responds to DEET and other repellents, as well as bitter quinine, through direct electrophysiological investigation. These gustatory receptor neuron responses prompted our sequencing of total mRNA from both male and female labella and tarsi samples to elucidate the putative chemoreception genes expressed in these contact chemoreception tissues. Samples of tarsi were divided into pro-, meso-and metathoracic subtypes for both sexes. We then validated our dataset by conducting qRT-PCR on the same tissue samples and used statistical methods to compare results between the two methods. Studies addressing molecular function may now target specific genes to determine those involved in repellent perception by mosquitoes. These receptor pathways may be used to screen novel repellents towards disruption of host-seeking behavior to curb the spread of harmful viruses. C1 [Sparks, Jackson T.; Dickens, Joseph C.] ARS, Henry A Wallace Beltsville Agr Res Ctr, Inst Plant Sci, Invas Insect Biocontrol & Behav Lab,USDA, Washington, DC 20250 USA. RP Dickens, JC (reprint author), ARS, Henry A Wallace Beltsville Agr Res Ctr, Inst Plant Sci, Invas Insect Biocontrol & Behav Lab,USDA, Washington, DC 20250 USA. EM Joseph.Dickens@ars.usda.gov FU Deployed War Fighter Protection (DWFP) Research Program - Department of Defense through the Armed Forces Pest Management Board (AFPMB) FX The authors thank Bryan T. Vinyard of the USDA, Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Biometrical Consulting Service, Beltsville, MD for statistical analyses. This work was supported in part by a grant to J.C.D. from the Deployed War Fighter Protection (DWFP) Research Program funded by the Department of Defense through the Armed Forces Pest Management Board (AFPMB). NR 18 TC 1 Z9 1 U1 6 U2 8 PU JOURNAL OF VISUALIZED EXPERIMENTS PI CAMBRIDGE PA 1 ALEWIFE CENTER, STE 200, CAMBRIDGE, MA 02140 USA SN 1940-087X J9 JOVE-J VIS EXP JI J. Vis. Exp. PD DEC PY 2014 IS 94 AR e52088 DI 10.3791/52088 PG 7 WC Multidisciplinary Sciences SC Science & Technology - Other Topics GA CB1AE UT WOS:000349358000025 ER PT J AU Della Valle, DM Glahn, RP AF Della Valle, Diane M. Glahn, Raymond P. TI Differences in relative iron bioavailability in traditional Bangladeshi meal plans SO FOOD AND NUTRITION BULLETIN LA English DT Article DE Bangladesh; bioavailability; Caco-2 cell culture; dal; iron; lentils; meal plan models; phytic acid; rice ID CELL-CULTURE MODEL; CACO-2 CELLS; YOUNG-WOMEN; PROTEIN DIGESTIBILITY; LENS-CULINARIS; COMMON BEANS; FOOD; COOKING; ABSORPTION; LENTILS AB Background. Iron deficiency is the most common nutrient deficiency worldwide. Large intakes of micronutrient-poor staple crops, coupled with low intakes of highly bioavailable dietary iron, are a major cause of this deficiency. Objective. To examine the concentration and relative bioavailability of iron in several models (n=23) of traditional Bangladeshi meals (rice, lentils/dal, vegetable, and fish), as well as the effect of removal of the lentil seed coat on the nutritional quality of iron. Methods. The relative bioavailability of iron was assessed by the in vitro/Caco-2 cell culture method, iron concentration by an inductively coupled argon-plasma emission spectrometer (ICAP-ES), and phytic acid concentration by colorimetric assay. The recipes contained 75% to 85% rice, 0% to 15% dal (containing whole or dehulled lentils), 0% to 15% vegetable curry, and 0% to 8% fish. Results. While the iron concentrations of recipes containing dehulled dal were significantly lower than those of recipes containing whole dal (p=.005), seed coat removal doubled relative iron bioavailability and increased phytic acid concentration (p<.001). The addition of fish to the meals had no significant effect on relative iron bioavailability. Iron concentration and relative iron bioavailability were correlated in the recipes containing dehulled dal (r=0.48, p=.03), but not whole dal (r=0.047, p=.84). Conclusions. The total amount of iron absorbed from traditional Bangladeshi meals is dependent upon iron concentration, and dehulling lentils removes inhibitory factors increasing iron uptake but also increases the density of phytic acid in the lentil sample. Thus, along with breeding for high iron concentration and bioavailability (i.e., biofortification), seed coat removal plus measures to lower phytic acid concentrations may be an important strategy to improve the bioavailability of iron in lentils and other pulse crops. C1 [Della Valle, Diane M.] Med Univ S Carolina, Charleston, SC 29425 USA. [Glahn, Raymond P.] Cornell Univ, USDA, ARS, Ithaca, NY USA. RP Glahn, RP (reprint author), USDA ARS, Robert W Holley Ctr Agr & Hlth, 538 Tower Rd, Ithaca, NY 14853 USA. EM rpg3@cornell.edu OI DellaValle, Diane/0000-0002-3855-432X FU Saskatchewan Pulse Growers; US Department of Agriculture, Agricultural Research Service FX Special thanks to Simpson Seeds Inc. in Moose Jaw, Saskatchewan, for the seed samples and to Bert Vandenberg, Brent Barlow, and their team at the University of Saskatchewan Crop Development Centre for dehulling the samples. We also thank Yongpei Chang for her technical support in the US Department of Agriculture Nutrition Laboratory and Mary Bodis and Ying Lu for technical assistance with the analysis of phytic acid concentration. Support for this research was provided by the Saskatchewan Pulse Growers and the US Department of Agriculture, Agricultural Research Service. NR 27 TC 4 Z9 4 U1 0 U2 8 PU INT NUTRITION FOUNDATION PI BOSTON PA 150 HARRISON AVE, BOSTON, MA 02111 USA SN 0379-5721 EI 1564-8265 J9 FOOD NUTR BULL JI Food Nutr. Bull. PD DEC PY 2014 VL 35 IS 4 BP 431 EP 439 PG 9 WC Food Science & Technology; Nutrition & Dietetics SC Food Science & Technology; Nutrition & Dietetics GA CA7ZC UT WOS:000349135300005 PM 25639128 ER PT J AU Lynn, DC Lesemann, RK Detro, JF Seery, JM AF Lynn, David C. Lesemann, Rebecca K. Detro, John F. Seery, Jason M. TI Employment of the "Role 2-Plus": Lessons Learned in a Time of High OPTEMPO SO MILITARY MEDICINE LA English DT Editorial Material AB From 2009 to 2014, the medical units at Fort Bragg, North Carolina, gained extensive experience in the nondoctrinal employment of the Forward Surgical Team (FST) combined with an Area Support Medical Company (ASMC) or Brigade Support Medical Company (BSMC). The setup and execution of the so-called "Role 2-plus" offers multiple operational and clinical advantages over solitary employment, but also presents some significant logistical challenges without proper planning. At the time of authorship, current literature describes this entity, (1) but information or guidance as to the actual employment of the Role 2-plus (including web-based outlets such as Center for Army Lessons Learned) is almost nonexistent. (1-6) Former commanders of these units would like to share and document some of the lessons learned during a time of exceptionally high operational tempo (OPTEMPO). C1 [Lynn, David C.] Womack Army Med Ctr, Dept Surg, Ft Bragg, NC 28310 USA. [Lesemann, Rebecca K.] Texas A&M Univ, Bush Sch Govt & Publ Serv, College Stn, TX 77843 USA. [Detro, John F.] USDA, XVIII Airborne Corps, Off Corps Surg, Ft Bragg, NC 28310 USA. [Seery, Jason M.] Jackson Mem Hosp, US Army Trauma Training Ctr, Ryder Trauma Ctr, Miami, FL 33136 USA. RP Lynn, DC (reprint author), Womack Army Med Ctr, Dept Surg, 2817 Reilly Rd, Ft Bragg, NC 28310 USA. NR 16 TC 0 Z9 0 U1 0 U2 0 PU ASSOC MILITARY SURG US PI BETHESDA PA 9320 OLD GEORGETOWN RD, BETHESDA, MD 20814 USA SN 0026-4075 EI 1930-613X J9 MIL MED JI Milit. Med. PD DEC PY 2014 VL 179 IS 12 BP 1412 EP 1418 DI 10.7205/MILMED-D-14-00099 PG 7 WC Medicine, General & Internal SC General & Internal Medicine GA CA7MB UT WOS:000349100300003 PM 25469961 ER PT J AU Aziz, I Mahmood, T Islam, KR AF Aziz, Irfan Mahmood, Tariq Islam, Khandakar Rafiq TI IMPACT OF LONG-TERM TILLAGE AND CROP ROTATION ON CONCENTRATION OF SOIL PARTICULATE ORGANIC MATTER ASSOCIATED CARBON AND NITROGEN SO PAKISTAN JOURNAL OF AGRICULTURAL SCIENCES LA English DT Article DE Tillage system; crop rotations; organic matter; soil management; soil texture ID ROOT-DERIVED CARBON; NO-TILLAGE; CONSERVATION TILLAGE; PHYSICAL PROTECTION; CULTIVATED SOILS; SURFACE RESIDUE; LIGHT FRACTION; LAND-USE; MANAGEMENT; AGGREGATE AB The soil management practices may pose intense effects on the nature and properties of soils. A field experiment was conducted during 2002 to 2007 on a Vanmeter farm of the Ohio State University South Centers at Piketon Ohio, USA to assess the long term influence of tillage practices and crop rotation on soil particulate organic matter allied with carbon and nitrogen. Tillage treatments including conventional (CT) and No-till (NT) were factored into continuous corn (CC), corn-soybean (CS) and corn-soybean-wheat-cowpea (CSW) rotations by following randomized complete block design with 6 replications. The results of long-term study showed that 12 percent of significant increase was observed in the particulate organic matter (POM) in NT by the passage of time. The particulate organic carbon (POC) concentration under NT increased (15 %) significantly when CT converted in to NT over five year. Furthermore, particulate organic nitrogen (PON) increased (12%) significantly under NT than under CT. Crop rotation had significant effects on POM and the effects were more prominent in the CSW rotation. The POM, POC concentration increased with time in CC (11%) and CSW (15%) rotations. As soil depth increases, significant decrease was observed in POM, POC and PON concentrations regardless of tillage and crop rotation treatments. Tillage and crop rotation had no significant influence on the physical parameters; however, with time as a factor in the interaction significantly influenced the physical properties. Changes in the physical properties of the soil by tillage practices and crop rotation are mainly due to soil disturbance, placement, amount and type of added biomass crop in the soil ecosystem. Physical properties of soil facilitate microbial activities, support chemical functions, improve protection C and improve the quality of soil. C1 [Aziz, Irfan] PMAS Arid Agr Univ, Dept Agron, Rawalpindi, Pakistan. [Mahmood, Tariq] PMAS Arid Agr Univ, Dept Environm Sci, Rawalpindi, Pakistan. [Islam, Khandakar Rafiq] Ohio State Univ, South Ctr, Piketon, OH USA. [Islam, Khandakar Rafiq] USDA ARS, Columbus, MS USA. RP Aziz, I (reprint author), PMAS Arid Agr Univ, Dept Agron, Rawalpindi, Pakistan. EM irfaz15@yahoo.com NR 59 TC 1 Z9 1 U1 1 U2 14 PU UNIV AGRICULTURE, FAC VETERINARY SCIENCE PI FAISALABAD PA UNIV AGRICULTURE, FAC VETERINARY SCIENCE, FAISALABAD, 00000, PAKISTAN SN 0552-9034 EI 2076-0906 J9 PAK J AGR SCI JI Pak. J. Agric. Sci. PD DEC PY 2014 VL 51 IS 4 BP 827 EP 834 PG 8 WC Agriculture, Multidisciplinary SC Agriculture GA CB3XF UT WOS:000349562000007 ER PT J AU Pachepsky, YA Guber, AK Yakirevich, AM Mckee, L Cady, RE Nicholson, TJ AF Pachepsky, Yakov A. Guber, Andrey K. Yakirevich, Alexander M. Mckee, Lynn Cady, Ralph E. Nicholson, Thomas J. TI Scaling and Pedotransfer in Numerical Simulations of Flow and Transport in Soils SO VADOSE ZONE JOURNAL LA English DT Article ID SATURATED HYDRAULIC CONDUCTIVITY; HETEROGENEOUS MEDIA; WATER-RETENTION; BULK-DENSITY; DEPENDENCY; AQUIFERS; INFILTROMETER; PATHWAYS; MOISTURE; TEXTURE AB Flow and transport parameters of soils in numerical simulations need to be defined at the support scale of computational grid cells. Such support scale can substantially differ from the support scale in laboratory or field measurements of flow and transport parameters. The scale dependence of flow and transport parameters essentially precludes the direct use of measured or pedotransfer-estimated parameter values in numerical simulations. The hypothesis of this work was that a support-based scaling law can be introduced that can convert pedotransfer-estimated saturated hydraulic conductivity values into values to be used over grid cells for finite-element-based simulations of water flow and tracer transport in variably saturated soils. A 4-month-long experiment was conducted at the USDA-ARS experimental site where Cl- as a tracer was applied with a pulse of irrigation water and its transport in groundwater and variably saturated shallow coarse-textured soils was monitored in two rows of wells on a daily basis. The HYDRUS-3D software was used to set and calibrate the Richards model for flow simulations and the convective-dispersive equation for transport simulations. Saturated hydraulic conductivity values were estimated with class pedotransfer functions derived from the USDA database containing results of about 1000 measurements in soils of different textures and bulk densities. A power law scaling for the saturated hydraulic conductivity was suggested based on literature data. When only two parameters of the scaling law rather than nine values of hydraulic conductivity from nine soil materials were calibrated, using the scaled saturated hydraulic conductivity values resulted in an accuracy of simulations that was similar to the accuracy of the calibrated model results. Upscaling of pedotransfer-estimated saturated hydraulic conductivities can provide reasonable estimates for numerical flow and transport modeling in variably saturated soils. C1 [Pachepsky, Yakov A.; Mckee, Lynn] USDA ARS, Beltsville Agr Res Ctr, Beltsville, MD 20705 USA. [Guber, Andrey K.] Michigan State Univ, Dep Plant Soil & Microbial Sci, E Lansing, MI 48824 USA. [Yakirevich, Alexander M.] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Dep Environm Hydrol & Microbiol, Negev, Israel. [Cady, Ralph E.; Nicholson, Thomas J.] US Nucl Regulatory Commiss, Off Nucl Regulatory Res, Rockville, MD 20852 USA. RP Pachepsky, YA (reprint author), USDA ARS, Beltsville Agr Res Ctr, 10300 Baltimore Ave, Beltsville, MD 20705 USA. EM yakov.pachepsky@ars.usda.gov OI Pachepsky, Yakov/0000-0003-0232-6090 FU Model Abstraction Techniques for Soil Water Flow and Transport [RES-02-008] FX This work was partially supported through the Interagency Agreement RES-02-008 "Model Abstraction Techniques for Soil Water Flow and Transport". NR 36 TC 6 Z9 6 U1 4 U2 13 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD DEC PY 2014 VL 13 IS 12 DI 10.2136/vzj2014.02.0020 PG 9 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CA9EA UT WOS:000349222000002 ER PT J AU Qu, W Bogena, HR Huisman, JA Martinez, G Pachepsky, YA Vereecken, H AF Qu, W. Bogena, H. R. Huisman, J. A. Martinez, G. Pachepsky, Y. A. Vereecken, H. TI Effects of Soil Hydraulic Properties on the Spatial Variability of Soil Water Content: Evidence from Sensor Network Data and Inverse SO VADOSE ZONE JOURNAL LA English DT Article ID REMOTE-SENSING FOOTPRINTS; TEMPORAL STABILITY; PEDOTRANSFER FUNCTIONS; FIELD-SCALE; SPATIOTEMPORAL VARIABILITY; MOISTURE VARIABILITY; PARAMETERS; TIME; DISTRIBUTIONS; DYNAMICS AB Understanding the spatial variation of soil water content and its relation with hydraulic properties is important for the application of hydrologic models. In this study, we investigated whether soil hydraulic properties are related to soil water content variability at the field scale. We used the mean relative difference ( MRD) of soil water content and saturation degree to describe the spatial soil water content variability of a 2- yr time series of soil water content measured with a sensor network at 41 locations in the Rollesbroich catchment ( Germany). Hydraulic properties were described by the van Genuchten- Mualem ( VGM) model and were estimated by inverse modeling of soil water content data using the Hydrus- 1D model for each location. The feasible parameter space for the inverse modeling was derived using the Rosetta pedotransfer functions from information on sand, silt, and clay contents and bulk density from 273 soil samples taken in the catchment. Our inverse modeling results were able to reproduce the observed time series of soil water content with a root mean square error < 0.08 cm3 cm- 3 and R2 > 0.75. We found that the MRDs of soil water content were positively correlated with the saturated water content ( qs) and shape- fitting n parameters and negatively correlated with the shape- fitting a and saturated hydraulic conductivity ( Ks) parameters of the VGM model. Moreover, the MRDs of the saturation degree were strongly correlated with the a and n parameters that determine the shape of the VGM model. C1 [Qu, W.; Bogena, H. R.; Huisman, J. A.; Vereecken, H.] Forschungszentrum Julich, IBG 3, Agrosphere Inst, D-52425 Julich, Germany. [Martinez, G.; Pachepsky, Y. A.] USDA ARS, Environm Microbial & Food Safety Lab, Beltsville, MD 20705 USA. [Martinez, G.] IFAPA, Ctr Alameda Obispo, Cordoba 14004, Spain. RP Qu, W (reprint author), Forschungszentrum Julich, IBG 3, Agrosphere Inst, D-52425 Julich, Germany. EM w.qu@fz-juelich.de RI Huisman, J.A. (Sander)/I-7078-2012 NR 70 TC 13 Z9 13 U1 10 U2 55 PU SOIL SCI SOC AMER PI MADISON PA 677 SOUTH SEGOE ROAD, MADISON, WI 53711 USA SN 1539-1663 J9 VADOSE ZONE J JI Vadose Zone J. PD DEC PY 2014 VL 13 IS 12 DI 10.2136/vzj2014.07.0099 PG 12 WC Environmental Sciences; Soil Science; Water Resources SC Environmental Sciences & Ecology; Agriculture; Water Resources GA CA9EA UT WOS:000349222000007 ER PT J AU Spooner, DM Ghislain, M Simon, R Jansky, SH Gavrilenko, T AF Spooner, David M. Ghislain, Marc Simon, Reinhard Jansky, Shelley H. Gavrilenko, Tatjana TI Systematics, Diversity, Genetics, and Evolution of Wild and Cultivated Potatoes SO BOTANICAL REVIEW LA English DT Article DE Domestication; Evolution; Genetics; Germplasm; Potato; Systematics; Taxonomy ID SOLANUM SECTION PETOTA; ENDOSPERM BALANCE NUMBER; TUBER-BEARING SOLANUMS; CYTOPLASMIC MALE-STERILITY; BIOGEOGRAPHIC PREDICTIVITY RESISTANCE; RESTRICTION-ENDONUCLEASE ANALYSIS; SIMPLE SEQUENCE REPEATS; INHIBITOR SLI GENE; UNILATERAL SEXUAL POLYPLOIDIZATION; GAMETOPHYTIC SELF-INCOMPATIBILITY AB The common potato, Solanum tuberosum L., is the third most important food crop and is grown and consumed worldwide. Indigenous cultivated (landrace) potatoes and wild potato species, all classified as Solanum section Petota, are widely used for potato improvement. Members of section Petota are broadly distributed in the Americas from the southwestern United States to the Southern Cone of South America. The latest comprehensive taxonomic treatment of section Petota was published by John (Jack) Hawkes in 1990; it recognized seven cultivated species and 228 wild species, divided into 21 taxonomic series. Since 1990, intensive field collections from throughout the range of the group, coupled with morphological and molecular studies, have halved the number of species and elucidated new ingroup and outgroup relationships. The recent sequencing of the potato genome has greatly accelerated investigation of all aspects of potato biology and allows us to address new questions not conceivable before. The purpose of this review is to provide a historical overview and update since 1990 of the systematics, diversity, genetics, domestication, evolution, and breeding of Solanum section Petota that will serve as a reference for the next generation of studies in the potato. C1 [Spooner, David M.; Jansky, Shelley H.] Univ Wisconsin, Dept Hort, USDA ARS, Madison, WI 53706 USA. [Ghislain, Marc] CIP, SSA Reg Off, Genom & Biotechnol Global Program, Nairobi 00603, Kenya. [Simon, Reinhard] Int Potato Ctr, Integrated IT & Computat Res Unit, Lima, Peru. [Gavrilenko, Tatjana] NI Vavilov Inst Plant Ind VIR, Dept Biotechnol, St Petersburg 190000, Russia. [Gavrilenko, Tatjana] St Petersburg State Univ, St Petersburg 199034, Russia. RP Spooner, DM (reprint author), Univ Wisconsin, Dept Hort, USDA ARS, Madison, WI 53706 USA. EM david.spooner@ars.usda.gov; m.ghislain@cgiar.org; r.simon@cgiar.org; shelley.jansky@ars.usda.gov; tatjana9972@yandex.ru RI Gavrilenko, Tatjana/G-8148-2015 OI Gavrilenko, Tatjana/0000-0002-2605-6569 FU United States Germplasm System; International Science and Technology Centre [ISTC 3329]; USDA-ARS National Plant Germplasm System Horticultural Evaluation Grants; USDA National Research Initiative grant [2008-35300-18669]; NSF [DEB 0316614] FX This study was made possible with the financial support of germplasm-collecting grants from the United States Germplasm System; the International Science and Technology Centre grant ISTC 3329; by USDA-ARS National Plant Germplasm System Horticultural Evaluation Grants; the USDA National Research Initiative grant number 2008-35300-18669; and NSF DEB 0316614 (PBI Solanum: A worldwide treatment). We thank John Bamberg and Kazuyoshi Hosaka for reviews of their contributions, and Sandra Knapp and one unidentified person for comments in the final review. We thank NRC Research Press for permission to publish Fig. 6. NR 600 TC 22 Z9 25 U1 11 U2 63 PU SPRINGER PI NEW YORK PA 233 SPRING ST, NEW YORK, NY 10013 USA SN 0006-8101 EI 1874-9372 J9 BOT REV JI Bot. Rev. PD DEC PY 2014 VL 80 IS 4 BP 283 EP 383 DI 10.1007/s12229-014-9146-y PG 101 WC Plant Sciences SC Plant Sciences GA CA1HB UT WOS:000348662300001 ER PT J AU Whatmore, AM Davison, N Cloeckaert, A Al Dahouk, S Zygmunt, MS Brew, SD Perrett, LL Koylass, MS Vergnaud, G Quance, C Scholz, HC Dick, EJ Hubbard, G Schlabritz-Loutsevitch, NE AF Whatmore, Adrian M. Davison, Nicholas Cloeckaert, Axel Al Dahouk, Sascha Zygmunt, Michel S. Brew, Simon D. Perrett, Lorraine L. Koylass, Mark S. Vergnaud, Gilles Quance, Christine Scholz, Holger C. Dick, Edward J., Jr. Hubbard, Gene Schlabritz-Loutsevitch, Natalia E. TI Brucella papionis sp nova, isolated from baboons (Papio spp.) SO INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY LA English DT Article ID MOLECULAR CHARACTERIZATION; GENUS BRUCELLA; INSERTION-SEQUENCE; VACCINE STRAINS; MARINE MAMMALS; IDENTIFICATION; PINNIPEDIALIS; OVIS; PCR; DIFFERENTIATION AB Two Gram-negative, non-motile, non-spore-forming coccoid bacteria (strains F8/08-60(T). and F8/08-61) isolated from clinical specimens obtained from baboons (Papio spp.) that had delivered stillborn offspring were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA gene sequence similarities, both strains, which possessed identical sequences, were assigned to the genus Brucella. This placement was confirmed by extended multilocus sequence analysis (MLSA), where both strains possessed identical sequences, and whole-genome sequencing of a representative isolate. All of the above analyses suggested that the two strains represent a novel lineage within the genus Brucella. The strains also possessed a unique profile when subjected to the phenotyping approach classically used to separate species of the genus Brucella, reacting only with Brucella A monospecific antiserum, being sensitive to the dyes thionin and fuchsin, being lysed by bacteriophage Wb, Bk2 and Fi phage at routine test dilution (RTD) but only partially sensitive to bacteriophage Tb, and with no requirement for CO2 and no production of H2S but strong urease activity. Biochemical profiling revealed a pattern of enzyme activity and metabolic capabilities distinct from existing species of the genus Brucella. Molecular analysis of the omp2 locus genes showed that both strains had a novel combination of two highly similar omp2b gene copies. The two strains shared a unique fingerprint profile of the multiple-copy Brucella-specific element IS711. Like MLSA, a multilocus variable number of tandem repeat analysis (MLVA) showed that the isolates clustered together very closely, but represent a distinct group within the genus Brucella. Isolates F8/08-60(T) and F8/08-6(T) could be distinguished clearly from all known species of the genus Brucella and their biovars by both phenotypic and molecular properties. Therefore, by applying the species concept for the genus Brucella suggested by the ICSP Subcommittee on the Taxonomy of Brucella, they represent a novel species within the genus Brucella, for which the name Bruaella papionis sp. nov. is proposed, with the type strain F8/0860(T) (=NCTC 13660(T)=CIRMBP 0958(T)). C1 [Whatmore, Adrian M.; Brew, Simon D.; Perrett, Lorraine L.; Koylass, Mark S.] AHVLA, Dept Bacteriol, OIE WHO FAO Brucellosis Reference Lab, Addlestone KT15 3NB, Surrey, England. [Davison, Nicholas] AHVLA, Truro TR4 9AD, England. [Cloeckaert, Axel; Zygmunt, Michel S.] INRA, Infectiol & Sante Publ UMR1282, F-37380 Nouzilly, France. [Cloeckaert, Axel; Zygmunt, Michel S.] Univ Tours, Infectiol & Sante Publ UMR1282, F-37000 Tours, France. [Al Dahouk, Sascha] BfR Fed Inst Risk Assessment, D-12277 Berlin, Germany. [Vergnaud, Gilles] Univ Paris 11, Inst Genet & Microbiol, UMR 8621, F-91405 Orsay, France. [Vergnaud, Gilles] CNRS, F-91405 Orsay, France. [Vergnaud, Gilles] DGA MRIS Mission Rech & Innovat Sci, F-92221 Bagneux, France. [Quance, Christine] USDA APHIS, Mycobacteria & Brucella Sect, Natl Vet Serv Labs, Ames, IA 50010 USA. [Scholz, Holger C.] Bundeswehr Inst Microbiol, D-80937 Munich, Germany. [Dick, Edward J., Jr.] Texas Biomed Res Inst, Southwest Natl Primate Res Ctr, San Antonio, TX USA. [Hubbard, Gene] Univ Texas Hlth Sci Ctr San Antonio, Dept Pathol, San Antonio, TX 78229 USA. [Schlabritz-Loutsevitch, Natalia E.] Univ Texas Hlth Sci Ctr San Antonio, Dept Obstet & Gynecol, San Antonio, TX 78229 USA. RP Whatmore, AM (reprint author), AHVLA, Dept Bacteriol, OIE WHO FAO Brucellosis Reference Lab, Woodham Lane, Addlestone KT15 3NB, Surrey, England. EM Adrian.Whatmore@ahvla.gsi.gov.uk RI Whatmore, Adrian/C-7744-2011; APHA, Staff publications/E-6082-2010; Vergnaud, Gilles/P-1304-2015; Perrett, Lorraine/C-8792-2011; OI Vergnaud, Gilles/0000-0003-0913-194X; Al Dahouk, Sascha/0000-0003-3835-0818 FU Southwest National Primate Research Center grant from the National Center for Research Resources, National Institutes of Health; Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health [P51 OD011133]; ORIP [1 C06 RR014578, 1 C06 RR015456]; Federal Institute for Risk Assessment (BfR project) [1322-503]; French 'Direction Generale de l'Armement' [Brucella REI2010 34 0003]; UK Department of Environment, Food and Rural Affairs (Defra) FX This investigation used resources that were supported by the Southwest National Primate Research Center grant P51 RR013986 from the National Center for Research Resources, National Institutes of Health, and which are currently supported by the Office of Research Infrastructure Programs (ORIP) of the National Institutes of Health through P51 OD011133 and conducted in facilities constructed with support from ORIP through grants 1 C06 RR014578 and 1 C06 RR015456. We thank Bill Cooley for undertaking the electron microscopy work, Yolande Hauck for the MLVA16 typing and Nelly Bernardet, Cornelia Gollner, Anna-Louisa Hauffe and Jakub Muchowski for technical assistance. The work of S. Al D. was supported by a grant from the Federal Institute for Risk Assessment (BfR project no. 1322-503). The work of A. C., M. Z. and G. V. benefited from a grant from the French 'Direction Generale de l'Armement' (grant Brucella REI2010 34 0003), which supports the development of tools for the tracing of dangerous pathogens. Brucellosis research and surveillance activities at AHVLA are funded by the UK Department of Environment, Food and Rural Affairs (Defra). NR 40 TC 22 Z9 26 U1 3 U2 12 PU SOC GENERAL MICROBIOLOGY PI READING PA MARLBOROUGH HOUSE, BASINGSTOKE RD, SPENCERS WOODS, READING RG7 1AG, BERKS, ENGLAND SN 1466-5026 EI 1466-5034 J9 INT J SYST EVOL MICR JI Int. J. Syst. Evol. Microbiol. PD DEC PY 2014 VL 64 BP 4120 EP 4128 DI 10.1099/ijs.0.065482-0 PN 12 PG 9 WC Microbiology SC Microbiology GA CA0QO UT WOS:000348622000031 PM 25242540 ER PT J AU Guttikonda, SK Valliyodan, B Neelakandan, AK Tran, LSP Kumar, R Quach, TN Voothuluru, P Gutierrez-Gonzalez, JJ Aldrich, DL Pallardy, SG Sharp, RE Ho, THD Nguyen, HT AF Guttikonda, Satish K. Valliyodan, Babu Neelakandan, Anjanasree K. Tran, Lam-Son Phan Kumar, Rajesh Quach, Truyen N. Voothuluru, Priyamvada Gutierrez-Gonzalez, Juan J. Aldrich, Donavan L. Pallardy, Stephen G. Sharp, Robert E. Ho, Tuan-Hua David Nguyen, Henry T. TI Overexpression of AtDREB1D transcription factor improves drought tolerance in soybean SO MOLECULAR BIOLOGY REPORTS LA English DT Article DE DREB; Drought tolerance; Environmental stresses; Cell membrane stability; Soybean; Transcription factor ID ORYZA-SATIVA L.; STRESS TOLERANCE; GENE-EXPRESSION; ABIOTIC STRESS; REGULATORY NETWORKS; FUNCTIONAL GENOMICS; SIGNAL-TRANSDUCTION; WATER-STRESS; DREB1A GENE; RICE PLANTS AB Drought is one of the major abiotic stresses that affect productivity in soybean (Glycine max L.) Several genes induced by drought stress include functional genes and regulatory transcription factors. The Arabidopsis thaliana DREB1D transcription factor driven by the constitutive and ABA-inducible promoters was introduced into soybean through Agrobacterium tumefaciens-mediated gene transfer. Several transgenic lines were generated and molecular analysis was performed to confirm transgene integration. Transgenic plants with an ABA-inducible promoter showed a 1.5- to two-fold increase of transgene expression under severe stress conditions. Under wellwatered conditions, transgenic plants with constitutive and ABA-inducible promoters showed reduced total leaf area and shoot biomass compared to non-transgenic plants. No significant differences in root length or root biomass were observed between transgenic and non-transgenic plants under non-stress conditions. When subjected to gradual water deficit, transgenic plants maintained higher relative water content because the transgenic lines used water more slowly as a result of reduced total leaf area. This caused them to wilt slower than non-transgenic plants. Transgenic plants showed differential drought tolerance responses with a significantly higher survival rate compared to nontransgenic plants when subjected to comparable severe water-deficit conditions. Moreover, the transgenic plants also showed improved drought tolerance by maintaining 17-24 % greater leaf cell membrane stability compared to non-transgenic plants. The results demonstrate the feasibility of engineering soybean for enhanced drought tolerance by expressing stress-responsive genes. C1 [Guttikonda, Satish K.; Valliyodan, Babu; Quach, Truyen N.; Voothuluru, Priyamvada; Gutierrez-Gonzalez, Juan J.; Aldrich, Donavan L.; Pallardy, Stephen G.; Sharp, Robert E.; Nguyen, Henry T.] Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO 65211 USA. [Guttikonda, Satish K.; Valliyodan, Babu; Quach, Truyen N.; Voothuluru, Priyamvada; Gutierrez-Gonzalez, Juan J.; Aldrich, Donavan L.; Pallardy, Stephen G.; Sharp, Robert E.; Nguyen, Henry T.] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA. [Guttikonda, Satish K.] Dow AgroSci, Regulatory Sci & Govt Affairs, Biotechnol Regulatory Sci, Indianapolis, IN USA. [Neelakandan, Anjanasree K.] Iowa State Univ, Dept Agron, Ames, IA USA. [Tran, Lam-Son Phan] RIKEN, Ctr Sustainable Resource Sci, Signaling Pathway Res Unit, Yokohama, Kanagawa, Japan. [Kumar, Rajesh] Natl Bur Plant Genet Resources, Natl Res Ctr DNA Fingerprinting, New Delhi 110012, India. [Quach, Truyen N.] Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE USA. [Gutierrez-Gonzalez, Juan J.] Univ Minnesota, ARS, USDA, Plant Sci Res Unit, St Paul, MN 55108 USA. [Gutierrez-Gonzalez, Juan J.] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN 55108 USA. [Ho, Tuan-Hua David] Washington Univ, Dept Biol, St Louis, MO 63130 USA. RP Nguyen, HT (reprint author), Univ Missouri, Natl Ctr Soybean Biotechnol, Columbia, MO 65211 USA. EM nguyenhenry@missouri.edu RI Tran, Lam-Son/A-1346-2009; OI Tran, Lam-Son/0000-0001-9883-9768; Gutierrez-Gonzalez, Juan/0000-0002-6795-6192; Valliyodan, Babu/0000-0001-9457-9508 FU United Soybean Board; Missouri Soybean Merchandising Council FX This work was supported by the United Soybean Board and the Missouri Soybean Merchandising Council funding to HTN. NR 50 TC 10 Z9 10 U1 2 U2 22 PU SPRINGER PI DORDRECHT PA VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS SN 0301-4851 EI 1573-4978 J9 MOL BIOL REP JI Mol. Biol. Rep. PD DEC PY 2014 VL 41 IS 12 BP 7995 EP 8008 DI 10.1007/s11033-014-3695-3 PG 14 WC Biochemistry & Molecular Biology SC Biochemistry & Molecular Biology GA CA6FQ UT WOS:000349005800030 PM 25192890 ER PT J AU Turechek, WW Roberts, PD Stansly, PA Webster, CG Kousik, S Adkins, S AF Turechek, William W. Roberts, Pamela D. Stansly, Philip A. Webster, Craig G. Kousik, S. Adkins, Scott TI Spatial and Temporal Analysis of Squash vein yellowing virus Infections in Watermelon SO PLANT DISEASE LA English DT Article ID STUNTING-DISORDER-VIRUS; DISEASE INCIDENCE; WHITEFLY TRANSMISSION; MIXED INFECTIONS; VINE DECLINE; LEAF-CURL; CUCURBITS; FLORIDA; CROPS; HOST AB Squash vein yellowing virus (SqVYV) is a whitefly-transmitted ipomovirus infecting watermelon and other cucurbits that was recently introduced to Florida. Effects on watermelon are devastating, with total vine collapse, often near harvest, and fruit rendered unmarketable by brown, discolored flesh. The epidemiology of SqVYV was studied in a I-ha field of 'Fiesta' watermelon over six growing seasons (1 to VI) to characterize the spatial patterning of disease and temporal rate of disease progress, as well as its association with Cucurbit leaf crumple virus (CuLCrV) and Cucurbit yellow stunting disorder virus (CYSDV), two additional whitefly-transmitted viruses that often occur with SqVYV. The field was scouted at regular intervals for the length of the season for incidence of virus and number of whiteflies. Incidence of SqVYV reached 100% during seasons I, II, and V and 20% during season III. SqVYV did not occur during seasons IV and VI. SqVYV progressed in a characteristic logistic fashion in seasons I, II, and V but less so in season Ill. The rate of disease progress was similar for the three seasons with high disease incidence, with an average value of 0.18. A positive correlation between the area under the disease progress curve and whitefly-days was found, where both progress curves were calculated as a function of thermal time (degree days, base 0 degrees C). SqVYV displayed significant but variable levels of aggregation, as indicated by its fit to the beta-binomial distribution, the binary power law, and ordinary runs analysis. Association analysis indicated that the viruses were largely transmitted independently. Results of this study provide epidemiological information that will be useful in the development of management strategies for SqVYV-induced vine decline, and provide new information for CuLCrV and CYSDV. C1 [Turechek, William W.; Stansly, Philip A.; Adkins, Scott] ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. [Roberts, Pamela D.; Stansly, Philip A.] Univ Florida, Southwest Florida Res & Educ Ctr, Immokalee, FL 34142 USA. [Kousik, S.] ARS, USDA, Vegetable Lab, Charleston, SC 29414 USA. RP Turechek, WW (reprint author), ARS, USDA, US Hort Res Lab, Ft Pierce, FL 34945 USA. EM william.turechek@ars.usda.gov FU United States Department of Agriculture Cooperative State Research, Education, and Extension Service SCRI [2008-04890]; National Watermelon Association FX We thank B. Kostyk for collecting whitefly counts, R. Sytsma for field maintenance, and C. Vanderspool for excellent technical assistance with virus diagnostic tests. Financial support was provided, in part, by the United States Department of Agriculture Cooperative State Research, Education, and Extension Service SCRI grant 2008-04890 and the National Watermelon Association. NR 35 TC 0 Z9 0 U1 3 U2 14 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2014 VL 98 IS 12 BP 1671 EP 1680 DI 10.1094/PDIS-10-13-1094-RE PG 10 WC Plant Sciences SC Plant Sciences GA CA3SY UT WOS:000348827600012 ER PT J AU Stewart, JE Kroese, D Tabima, JF Larsen, MM Fieland, VJ Press, CM Zasada, IA Grunwald, NJ AF Stewart, Jane E. Kroese, Duncan Tabima, Javier F. Larsen, Meredith M. Fieland, Valerie J. Press, Caroline M. Zasada, Inga A. Gruenwald, Niklaus J. TI Pathogenicity, Fungicide Resistance, and Genetic Variability of Phytophthora rubi Isolates from Raspberry (Rubus idaeus) in the Western United States SO PLANT DISEASE LA English DT Article ID ROOT-ROT; RED RASPBERRY; POPULATIONS; IDENTITIES; INFESTANS; METALAXYL; FIELD AB Root rot of raspberry (Rubus idaeus), thought to be primarily caused by Phytophthora rubi, is an economically important disease in the western United States. The objectives of this study were to determine which Phytophthora species are involved in root rot, examine the efficacy of different isolation methods (cane, root, and root/soil baiting with young raspberry plants), and determine if pathogenicity, fungicide resistance, and/or genetic variation exists among P rubi isolates collected from raspberry fields in Washington, Oregon, and California. Of 275 samples, direct isolation from cane material resulted in a greater number of P rubi isolates (39%), whereas root/soil baiting yielded the least (11%). Sequencing of the internal transcribed spacer region of 210 of the total 597 collected Phytophthora isolates showed that all but one isolate (identified as P. bisheria) were P rubi. Results of the pathogenicity and fungicide resistance to mefenoxam comparing 14 total isolates from Washington, Oregon, and California showed that isolates were similarly virulent against red raspberry and the EC50 frequency distributions showed no significant difference. These results, combined with amplified fragment length polymorphism results show that P rubi isolates from Washington, Oregon, and California represent one large mixed population. This work provides novel insights into the isolation and biology of P rubi in western U.S. raspberry production systems. C1 [Stewart, Jane E.; Kroese, Duncan; Larsen, Meredith M.; Press, Caroline M.; Zasada, Inga A.; Gruenwald, Niklaus J.] ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. [Tabima, Javier F.; Fieland, Valerie J.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97330 USA. RP Grunwald, NJ (reprint author), ARS, USDA, Hort Crops Res Lab, Corvallis, OR 97330 USA. EM Nik.Grunwald@ARS.USDA.GOV RI Grunwald, Niklaus/K-6041-2013; OI Grunwald, Niklaus/0000-0003-1656-7602; Tabima, Javier/0000-0002-3603-2691 FU USDA ARS CRIS [5358-22000-039-00D]; USDA-NIFA-RAMP [2010-511001-21649] FX We thank Jenny Broome and the Driscolls Team and Mark Bolda, University of California Extension, for help in sample collections and isolation method ideas. We thank Tom Walters and Peerbolt Crop Management for help with collections, and Karan Fairchild and Kim Hens lee for excellent laboratory assistance. This work was supported by funds from USDA ARS CRIS Project 5358-22000-039-00D and USDA-NIFA-RAMP Project 2010-511001-21649. Mention of trade names or commercial products in this manuscript is solely for the purpose of providing specific information and does not imply recommendation or endorsement. NR 39 TC 0 Z9 0 U1 0 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2014 VL 98 IS 12 BP 1702 EP 1708 DI 10.1094/PDIS-11-13-1130-RE PG 7 WC Plant Sciences SC Plant Sciences GA CA3SY UT WOS:000348827600016 ER PT J AU Eyre, CA Hayden, KJ Kozanitas, M Grunwald, NJ Garbelotto, M AF Eyre, C. A. Hayden, K. J. Kozanitas, M. Gruenwald, N. J. Garbelotto, M. TI Lineage, Temperature, and Host Species have Interacting Effects on Lesion Development in Phytophthora ramorum SO PLANT DISEASE LA English DT Article ID SUDDEN OAK DEATH; 3 CLONAL LINEAGES; NORTH-AMERICAN; UNITED-STATES; US NURSERIES; IN-VITRO; MICROSATELLITE MARKERS; PLANT PATHOGEN; FOREST DISEASE; MATING-TYPE AB There are four recognized clonal lineages of the pathogen Phytophthora ramorum. The two major lineages present in North America are NA1 and NA2. With a few exceptions, NA1 is found in natural forest ecosystems and nurseries, and NA2 is generally restricted to nurseries. Isolates from the NA1 and NA2 lineages were used to infect rhododendron, camellia, and California bay laurel in detached leaf assays to study the effects of lineage, temperature, and host on pathogenicity and host susceptibility. Isolates within both lineages were highly variable in their ability to form lesions on each host. There was also a tendency toward reduced lesion size in successive trials, suggesting degeneration of isolates over time. Temperature had a significant effect on lesion size, with a response that varied depending on the host and isolate. Phenotypic differences between lineages appear to be heavily influenced by the representation of isolates used, host, and temperature. The importance of temperature, host, and lineage are discussed with respect to disease management, as well as future range expansions and migrations of the pathogen. C1 [Eyre, C. A.; Hayden, K. J.; Kozanitas, M.; Garbelotto, M.] Univ Calif Berkeley, Forest Pathol & Mycol Lab, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. [Hayden, K. J.] INRA, UMR 1136, F-54280 Champenoux, France. [Hayden, K. J.] Univ Lorraine, UMR 1136, F-54500 Vandoeuvre Les Nancy, France. [Gruenwald, N. J.] ARS, Hort Crops Res Lab, USDA, Corvallis, OR USA. RP Garbelotto, M (reprint author), Univ Calif Berkeley, Forest Pathol & Mycol Lab, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA. EM matteog@berkeley.edu RI Grunwald, Niklaus/K-6041-2013 OI Grunwald, Niklaus/0000-0003-1656-7602 FU United States Department of Agriculture (USDA) Forest Service; Pacific Southwest Research Station; NSF-Ecology of Infectious Diseases program [1115607]; USDA Agricultural Research Service CRIS [5358-22000-039-00D] FX This study was funded by the United States Department of Agriculture (USDA) Forest Service, Pacific Southwest Research Station, and by the NSF-Ecology of Infectious Diseases program, Award Number 1115607. It was also supported by funds from USDA Agricultural Research Service CRIS Project 5358-22000-039-00D. We thank K. Graham, M. Larsen, M. Dimas, and M. Liu for lab assistance. NR 78 TC 2 Z9 2 U1 4 U2 18 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2014 VL 98 IS 12 BP 1717 EP 1727 DI 10.1094/PDIS-02-14-0151-RE PG 11 WC Plant Sciences SC Plant Sciences GA CA3SY UT WOS:000348827600018 ER PT J AU Elliott, M Chastagner, GA Coats, KP Sikdar, P Xiao, CL AF Elliott, M. Chastagner, G. A. Coats, K. P. Sikdar, P. Xiao, C. L. TI First Report of a New Leaf Blight Caused by Phacidiopycnis washingtonensis on Pacific Madrone in Western Washington and Oregon SO PLANT DISEASE LA English DT News Item C1 [Elliott, M.; Chastagner, G. A.; Coats, K. P.] Washington State Univ, Res & Extens Ctr, Dept Plant Pathol, Puyallup, WA 98371 USA. [Sikdar, P.] Washington State Univ, Dept Plant Pathol, Tree Fruit Res & Extens Ctr, Wenatchee, WA 98801 USA. [Xiao, C. L.] ARS, USDA, San Joaquin Valley Agr Sci Ctr, Parlier, CA 93648 USA. RP Elliott, M (reprint author), Washington State Univ, Res & Extens Ctr, Dept Plant Pathol, Puyallup, WA 98371 USA. NR 2 TC 0 Z9 0 U1 1 U2 4 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2014 VL 98 IS 12 BP 1741 EP + DI 10.1094/PDIS-05-14-0462-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CA3SY UT WOS:000348827600026 ER PT J AU Li, MR Zhu, J Gao, J Li, RH Li, F AF Li, M. R. Zhu, J. Gao, J. Li, R. H. Li, F. TI First Report of Carnation vein mottle virus Infecting Dianthus amurensis in China SO PLANT DISEASE LA English DT News Item C1 [Li, M. R.; Zhu, J.; Li, F.] Yunnan Agr Univ, Key Lab Agr Biodivers Pest Management, China Educ Minist, Kunming 650201, Peoples R China. [Gao, J.] Jilin Agr Univ, Dept Plant Pathol, Coll Agron, Changchun 130118, Peoples R China. [Li, R. H.] ARS, USDA, Natl Germplasm Resources Lab, Beltsville, MD 20705 USA. RP Li, MR (reprint author), Yunnan Agr Univ, Key Lab Agr Biodivers Pest Management, China Educ Minist, Kunming 650201, Peoples R China. NR 3 TC 0 Z9 0 U1 1 U2 5 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2014 VL 98 IS 12 DI 10.1094/PDIS-05-14-0453-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CA3SY UT WOS:000348827600043 ER PT J AU Saito, S Michailides, TJ Xiao, CL AF Saito, S. Michailides, T. J. Xiao, C. L. TI First Report of Botrytis pseudocinerea Causing Gray Mold on Blueberry in North America SO PLANT DISEASE LA English DT News Item ID VINEYARDS; CINEREA C1 [Saito, S.] USDA, San Joaquin Agr Sci Ctr, Parlier, CA 93648 USA. [Saito, S.] Univ California, Kearney Agr Res Ctr, Parlier, CA 93648 USA. [Michailides, T. J.] Univ Calif Davis, Kearney Agr Res & Extens Ctr, Dept Plant Pathol, Parlier, CA 93648 USA. [Xiao, C. L.] ARS, USDA, San Joaquin Agr Sci Ctr, Parlier, CA 93648 USA. RP Saito, S (reprint author), USDA, San Joaquin Agr Sci Ctr, Parlier, CA 93648 USA. NR 4 TC 5 Z9 6 U1 3 U2 12 PU AMER PHYTOPATHOLOGICAL SOC PI ST PAUL PA 3340 PILOT KNOB ROAD, ST PAUL, MN 55121 USA SN 0191-2917 EI 1943-7692 J9 PLANT DIS JI PLANT DIS. PD DEC PY 2014 VL 98 IS 12 DI 10.1094/PDIS-06-14-0573-PDN PG 2 WC Plant Sciences SC Plant Sciences GA CA3SY UT WOS:000348827600033 ER PT J AU White, SD Hart, JL Cox, LE Schweitzer, CJ AF White, Stephen D. Hart, Justin L. Cox, Lauren E. Schweitzer, Callie J. TI Woody Regeneration in a Southern Appalachian Quercus Stand Following Wind Disturbance and Salvage Logging SO CASTANEA LA English DT Article DE Acer rubrum; compounded disturbance; Quercus; regeneration; salvage logging ID EASTERN UNITED-STATES; CANOPY ACCESSION STRATEGIES; OAK FORESTS; RED MAPLE; ACCELERATED SUCCESSION; CUMBERLAND PLATEAU; PRESCRIBED FIRE; NORTH-AMERICA; GROWTH; PATTERNS AB In the eastern United States, the practice of salvage logging is common to reclaim economic losses and/or reduce fuel loading following a natural disturbance. A current hypothesis states that two disturbances in rapid succession (i.e., compounded disturbance) have a cumulative severity of impact and may displace the successional trajectory further than either disturbance occurring separately. On 20 April 2011, Bankhead National Forest in Alabama was affected by an EF1 tornado with accompanying straight-line winds. Much of the damage was salvaged, however adjacent disturbance in a Wilderness Area was not harvested. A unique set of conditions allowed for comparisons of woody regeneration within a single stand, reducing uncontrolled variables to test the compounded disturbance hypothesis at a fine spatial scale. After two growing seasons, species richness, species evenness, and Shannon diversity of seedlings and saplings did not significantly differ by disturbance class. We found seedling density significantly differed between functional groups and we noted a significant interaction between functional groups and disturbance classes. In the sapling layer, density was significantly different among functional groups and among disturbance classes, but our results did not reveal a significant interaction between these factors. The wind disturbance accelerated succession in the Quercus stand toward dominance by shade-tolerant species, notably A. rubrum, and the salvage operation amplified the Quercus-to-Acer transition. However, even in wind-disturbed areas that were not salvaged, the regeneration model predicted A. rubrum to have more than twice the density of all combined Quercus species in the future stand. C1 [White, Stephen D.; Hart, Justin L.; Cox, Lauren E.] Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. [Schweitzer, Callie J.] US Forest Serv, Southern Res Stn, USDA, Huntsville, AL 35762 USA. RP Hart, JL (reprint author), Univ Alabama, Dept Geog, Tuscaloosa, AL 35487 USA. EM hart013@ua.edu RI Hart, Justin/B-2535-2010 OI Hart, Justin/0000-0001-6580-0189 NR 49 TC 1 Z9 1 U1 5 U2 16 PU SOUTHERN APPALACHIAN BOTANICAL SOC, NEWBERRY COLL PI NEWBERRY PA DEPT BIOLOGY, C/O CHARLES N HORN, SECRETARY-TREASURER, 2100 COLLEGE ST, NEWBERRY, SC 29108 USA SN 0008-7475 EI 1938-4386 J9 CASTANEA JI Castanea PD DEC PY 2014 VL 79 IS 4 BP 223 EP 236 DI 10.2179/14-009 PG 14 WC Plant Sciences SC Plant Sciences GA CA2WO UT WOS:000348766600002 ER PT J AU Russell, MB D'Amato, AW Schulz, BK Woodall, CW Domke, GM Bradford, JB AF Russell, Matthew B. D'Amato, Anthony W. Schulz, Bethany K. Woodall, Christopher W. Domke, Grant M. Bradford, John B. TI Quantifying understorey vegetation in the US Lake States: a proposed framework to inform regional forest carbon stocks SO FORESTRY LA English DT Article ID UNITED-STATES; NORTHERN MINNESOTA; SOUTHEAST ALASKA; BIOMASS; ECOSYSTEMS; COVER; MODELS; REGRESSION; STAND; PINE AB The contribution of understorey vegetation (UVEG) to forest ecosystem biomass and carbon (C) across diverse forest types has, to date, eluded quantification at regional and national scales. Efforts to quantify UVEG C have been limited to field-intensive studies or broad-scale modelling approaches lacking field measurements. Although large-scale inventories of UVEG C are not common, species-and community-level inventories of vegetation structure are available and may prove useful in quantifying UVEG C stocks. This analysis developed a general framework for estimating UVEG C stocks by employing per cent cover estimates of UVEG from a region-wide forest inventory coupled with an estimate of maximum UVEG C across the US Lake States (i.e. Michigan, Minnesota and Wisconsin). Estimates of UVEG C stocks from this approach reasonably align with expected C stocks in the study region, ranging from 0.86 +/- 0.06 Mg ha(-1) in red pine-dominated to 1.59 +/- 0.06 Mg ha(-1) for aspen/birch-dominated forest types. Although the data employed here were originally collected to assess broad-scale forest structure and diversity, this study proposes a framework for using UVEG inventories as a foundation for estimating C stocks in an often overlooked, yet important ecosystem C pool. C1 [Russell, Matthew B.; D'Amato, Anthony W.] Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. [Schulz, Bethany K.] US Forest Serv, USDA, Pacific NW Res Stn, Anchorage, AK 99503 USA. [Woodall, Christopher W.; Domke, Grant M.] US Forest Serv, USDA, No Res Stn, St Paul, MN 55108 USA. [Bradford, John B.] US Geol Survey, Southwest Biol Sci Ctr, Flagstaff, AZ 86011 USA. RP Russell, MB (reprint author), Univ Minnesota, Dept Forest Resources, St Paul, MN 55108 USA. EM russellm@umn.edu RI Bradford, John/E-5545-2011; OI Domke, Grant/0000-0003-0485-0355 FU USDA Forest Service - Northern Research Station FX This work was supported by the USDA Forest Service - Northern Research Station. We thank Andrew Gray, Coeli Hoover and Paul Van Deusen for comments on an earlier draft of this manuscript. We appreciate comments from Gary Kerr, Geoff Morgan and two anonymous reviewers for their insight that improved the content of this work. NR 57 TC 0 Z9 1 U1 3 U2 14 PU OXFORD UNIV PRESS PI OXFORD PA GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND SN 0015-752X EI 1464-3626 J9 FORESTRY JI Forestry PD DEC PY 2014 VL 87 IS 5 BP 629 EP 638 DI 10.1093/forestry/cpu023 PG 10 WC Forestry SC Forestry GA AZ6OW UT WOS:000348339700003 ER PT J AU Fennimore, SA Martin, FN Miller, TC Broome, JC Dorn, N Greene, I AF Fennimore, Steven A. Martin, Frank N. Miller, Thomas C. Broome, Janet C. Dorn, Nathan Greene, Ian TI Evaluation of a Mobile Steam Applicator for Soil Disinfestation in California Strawberry SO HORTSCIENCE LA English DT Article DE ammonium; fumigation alternatives; nitrate; pathogen suppression; weed control ID METHYL-BROMIDE FUMIGATION; THERMAL DEATH; FUSARIUM-OXYSPORUM; WEED-CONTROL; TEMPERATURE; GROWTH; PATHOGENS; RECOLONIZATION; STERILIZATION; IRRIGATION AB Steam-disinfestation of soil as an alternative to chemical fumigation was investigated in both research and commercial strawberry (Fragaria x ananassa Duch.) production field trials at four sites over 2 years (2011-13) using new prototype commercial application equipment: a tractor-drawn device that physically mixed the steam with the soil as it passed through the shaped planting beds. Results included significant suppression of weeds and soilborne pathogens equal to commercial chemigation of chloropicrin with 1,3-dichloropropene (Pic-Clor 60). Also, the combination of steam treatment with soil amendments of mustard seed meal (MSM; two of four trials included treatment), a fertilizer and source of additional organic matter, showed very favorable strawberry production in terms of yield as Well as weed and pathogen control. Soil nitrogen-containing ions were monitored at two of the sites and the MSM treatment significantly elevated available soil nitrates by the time of transplanting as did the steam treatment alone, but only significantly at one of the sites. C1 [Fennimore, Steven A.; Miller, Thomas C.] Univ Calif Davis, Dept Plant Sci, Salinas, CA 93905 USA. [Martin, Frank N.] ARS, USDA, Salinas, CA 93905 USA. [Broome, Janet C.] Driscoll Strawberry Associates, Watsonville, CA 95076 USA. [Dorn, Nathan] Reiter Affiliated Co, Salinas, CA 93907 USA. [Greene, Ian] RAMCO & Norcal, Salinas, CA 93905 USA. RP Fennimore, SA (reprint author), Univ Calif Davis, Dept Plant Sci, 1636 East Alisal St, Salinas, CA 93905 USA. EM safennimore@ucdavis.edu FU USDA NIFA [2010-51102-21648]; California Strawberry Commission; Propane Education and Research Council FX Funding for these studies included support awarded in USDA NIFA Methyl Bromide Transitions grant 2010-51102-21648, the California Strawberry Commission, The Propane Education and Research Council, and in-kind support from Driscoll's Strawberry Associates and Reiter Affiliated Companies (Watsonville, CA) and their capable staff of engineering and operations. NR 50 TC 3 Z9 3 U1 4 U2 13 PU AMER SOC HORTICULTURAL SCIENCE PI ALEXANDRIA PA 113 S WEST ST, STE 200, ALEXANDRIA, VA 22314-2851 USA SN 0018-5345 EI 2327-9834 J9 HORTSCIENCE JI Hortscience PD DEC PY 2014 VL 49 IS 12 BP 1542 EP 1549 PG 8 WC Horticulture SC Agriculture GA AZ1OP UT WOS:000348008400011 ER PT J AU Fox, RJ Fisher, TR Gustafson, AB Jordan, TE Kana, TM Lang, MW AF Fox, R. J. Fisher, T. R. Gustafson, A. B. Jordan, T. E. Kana, T. M. Lang, M. W. TI Searching for the missing nitrogen: biogenic nitrogen gases in groundwater and streams SO JOURNAL OF AGRICULTURAL SCIENCE LA English DT Article ID COASTAL-PLAIN WATERSHEDS; CHOPTANK RIVER-BASIN; CHESAPEAKE BAY; NORTHEASTERN USA; UNITED-STATES; DENITRIFICATION; NITRATE; FLUXES; EXPORT; ECOSYSTEMS AB Biogenic nitrogen (N-2) and nitrous oxide (N2O ) accumulations were measured in groundwater, streams and the vadose zone of small agricultural watersheds in the Mid-Atlantic USA. In general, N-2 and N2O in excess of atmospheric equilibrium were found in groundwater virtually everywhere that was sampled. Excess N-2 in groundwater ranged from undetectable to 616 mu mol N-2-N/l, the latter representing c. 50% of background N-2. The N2O-N concentrations varied from undetectable to 75 mu M, and usually greatly exceeded values at atmospheric equilibrium (25-30 nM); however, N2O was generally 1-10% of excess N-2. Intermediate levels of deficit and excess N-2 in flowing streams (-65 to +250 mu mol N-2-N/L) resulting from both abiotic and biotic processes were also measured. In vadose zone gases, multiple N-2/Ar gas profiles were measured which exhibited seasonal variations with below atmospheric values when the soil was warming in spring/summer and above atmospheric values when groundwater was cooling in fall/winter. Both abiotic and biotic processes contributed to the excess N-2 and N2O that was observed. The current data indicate that large concentrations of excess N gases can accumulate within soil, groundwater, and streams of agriculturally dominated watersheds. When excess N gases are exchanged with the atmosphere, the net fluxes to the atmosphere may represent an important loss term for watershed N budgets. C1 [Fox, R. J.; Fisher, T. R.; Gustafson, A. B.; Kana, T. M.] Univ Maryland, Ctr Environm Sci, Horn Point Lab, Cambridge, MD 21613 USA. [Jordan, T. E.] Smithsonian Environm Res Ctr, Edgewater, MD 21037 USA. [Lang, M. W.] USDA, Forest Serv, No Res Stn, Beltsville, MD 20705 USA. RP Fox, RJ (reprint author), Univ Maryland, Ctr Environm Sci, Horn Point Lab, Cambridge, MD 21613 USA. EM rfox@umces.edu FU USDA Cooperative State Research, Education, and Extension Service (CSREES) [2005-35102-16299, 2008-51130-04898]; NSF Ecosystem Studies Programme [DEB-091981 ARRA, DEB-091941 ARRA, DEB-1252923]; Washington D.C. Explorers Club for Exploration and Field Research; University of Maryland Graduate Student Summer Research Fellowship; Horn Point Laboratory Education Fund FX We thank Joe Miklas, Alley Bullock, Nancy Goff, Katie Bentley, Lindsay Tempinson, Shadaesha Greene and Whitley Gilbert for collection of stream and groundwater samples. We also thank Dana Brenner, Karen Knee and John Gardner for additional laboratory and field support. Dana Bunnell-Young created Fig. 2. Financial support was provided by the USDA Cooperative State Research, Education, and Extension Service (CSREES, 2005-35102-16299 to TRF, 2008-51130-04898 to TRF) and NSF Ecosystem Studies Programme (DEB-091981 ARRA to TRF, DEB-091941 ARRA to TEJ and DEB-1252923 to TRF and RJF). RJF was additionally funded by a grant from the Washington D.C. Explorers Club for Exploration and Field Research, the University of Maryland Graduate Student Summer Research Fellowship and the Horn Point Laboratory Education Fund. This is University of Maryland Center for Environmental Science contribution number 4871. NR 40 TC 1 Z9 1 U1 0 U2 11 PU CAMBRIDGE UNIV PRESS PI NEW YORK PA 32 AVENUE OF THE AMERICAS, NEW YORK, NY 10013-2473 USA SN 0021-8596 EI 1469-5146 J9 J AGR SCI-CAMBRIDGE JI J. Agric. Sci. PD DEC PY 2014 VL 152 SU 1 SI SI BP S96 EP S106 DI 10.1017/S0021859614000070 PG 11 WC Agriculture, Multidisciplinary SC Agriculture GA AY6ZI UT WOS:000347710700011 ER PT J AU Howland, AD Schreiner, RP Zasada, IA AF Howland, Amanda D. Schreiner, R. Paul Zasada, Inga A. TI Spatial Distribution of Plant-Parasitic Nematodes in Semi-Arid Vitis vinifera Vineyards in Washington SO JOURNAL OF NEMATOLOGY LA English DT Article DE arbuscular mycorrhizal fungi colonization; management; plant-parasitic nematodes; semi-arid; spatial distribution; Vitis vinifera; Washington ID ROOT-KNOT NEMATODES; MYCORRHIZAL FUNGI; PRATYLENCHUS SPP.; GRAPEVINE; SOIL; COLONIZATION; ROOTSTOCKS; RESISTANT; XENOPLAX; CLIMATE AB The most commonly encountered plant-parasitic nematodes in eastern Washington Vitis vinifera vineyards are Meloidogyne hapla, Mesocriconema xenoplax, Pratylenchus spp., Xiphinema americanum, and Paratylenchus sp.; however, little is known about their distribution in the soil profile. The vertical and horizontal spatial distribution of plant-parasitic nematodes was determined in two Washington V. vinifera vineyards. Others variables measured in these vineyards included soil moisture content, fine root biomass, and root colonization by arbuscular mycorhizal fungi (AMF). Meloidogyne hapla and M. xenoplax were aggregated under irrigation emitters within the vine row and decreased with soil depth. Conversely, Pratylenchus spp. populations were primarily concentrated in vineyard alleyways and decreased with depth. Paratylenchus sp. and X. americanum were randomly distributed within the vineyards. Soil water content played a dominant role in the distribution of fine roots and plant-parasitic nematodes. Colonization of fine roots by AMF decreased directly under irrigation emitters; in addition, galled roots had lower levels of AMF colonization compared with healthy roots. These findings will help facilitate sampling and management decisions for plant-parasitic nematodes in Washington semi-arid vineyards. C1 [Howland, Amanda D.] Oregon State Univ, Dept Hort, Corvallis, OR 97330 USA. [Schreiner, R. Paul; Zasada, Inga A.] USDA ARS, Hort Crops Res Lab, Corvallis, OR 97330 USA. RP Howland, AD (reprint author), Oregon State Univ, Dept Hort, 4017 Agr & Life Sci Bldg, Corvallis, OR 97330 USA. EM Inga.Zasada@ars.usda.gov FU Washington Grape & Wine Research Program; Ste. Michelle Wine Estates FX The authors acknowledge the Washington Grape & Wine Research Program for partially funding this research and Ste. Michelle Wine Estates for their support. We also thank Jack Pinkerton, John Wilson, Duncan Kroese, and Marj Lauer for technical assistance. NR 35 TC 3 Z9 3 U1 3 U2 11 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD DEC PY 2014 VL 46 IS 4 BP 321 EP 330 PG 10 WC Zoology SC Zoology GA CA1CE UT WOS:000348649800002 PM 25580024 ER PT J AU Shapiro-Ilan, DI Blackburn, D Duncan, L El-Borai, FE Koppenhofer, H Tailliez, P Adams, BJ AF Shapiro-Ilan, David I. Blackburn, Dana Duncan, Larry El-Borai, Fahiem E. Koppenhoefer, Heather Tailliez, Patrick Adams, Byron J. TI Characterization of Biocontrol Traits in Heterorhabditis floridensis: A Species with Broad Temperature Tolerance SO JOURNAL OF NEMATOLOGY LA English DT Article DE beneficial trait; biological control; entomopathogenic nematode; Heterorhabditis floridensis ID N-SP RHABDITIDA; ADAPTED ENTOMOPATHOGENIC NEMATODE; DIAPREPES-ABBREVIATUS COLEOPTERA; ECOLOGICAL CHARACTERIZATION; GENUS PHOTORHABDUS; BIOLOGICAL-CONTROL; PLUM CURCULIO; SUBSP NOV; STEINERNEMATIDAE; STRAIN AB Biological characteristics of two strains of the entomopathogenic nematode, Heterorhabditis floridensis (332 isolated in Florida and K22 isolated in Georgia) were described. The identity of the nematode's symbiotic bacteria was elucidated and found to be Photorhabdus luminescens subsp. luminescens. Beneficial traits pertinent to biocontrol (environmental tolerance and virulence) were characterized. The range of temperature tolerance in the H. floridensis strains was broad and showed a high level of heat tolerance. The H. floridensis strains caused higher mortality or infection in G. mellonella at 30 degrees C and 35 degrees C compared with S. riobrave (355), a strain widely known to be heat tolerant, and the H. floridensis strains were also capable of infecting at 17 degrees C whereas S. riobrave (355) was not. However, at higher temperatures (37 degrees C and 39 degrees C), though H. floridensis readily infected G. mellonella, S. riobrave strains caused higher levels of mortality. Desiccation tolerance in H. floridensis was similar to Heterorhabditis indica (Hom1) and S. riobrave (355) and superior to S. feltiae (SN). H. bacteriophora (Oswego) and S. carpocapsae (All) exhibited higher desiccation tolerance than the H. floridensis strains. The virulence of H. floridensis to four insect pests (Aethina tumida, Conotrachelus nenuphar, Diaprepes abbreviatus, and Tenebrio molitor) was determined relative to seven other nematodes: H. bacteriophora (Oswego), H. indica (Hom1), S. carpocapsae (All), S. feltiae (SN), S. glaseri (4-8 and Vs strains), and S. riobrave (355). Virulence to A. tumida was similar among the H. floridensis strains and other nematodes except S. glaseri (Vs), S. feltiae, and S. riobrave failed to cause higher mortality than the control. Only H. bacteriophora, H. indica, S. feltiae, S. riobrave, and S. glaseri (4-8) caused higher mortality than the control in C. nenuphar. All nematodes were pathogenic to D. abbreviatus though S. glaseri (4-8) and S. riobrave (355) were the most virulent. S. carpocapsae was the most virulent to T. molitor. In summary, the H. floridensis strains possess a wide niche breadth in temperature tolerance and have virulence and desiccation levels that are similar to a number of other entomopathogenic nematodes. The strains may be useful for biocontrol purposes in environments where temperature extremes occur within short durations. C1 [Shapiro-Ilan, David I.] USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. [Blackburn, Dana; Adams, Byron J.] Brigham Young Univ, Dept Biol, Provo, UT 84602 USA. [Blackburn, Dana; Adams, Byron J.] Brigham Young Univ, Evolutionary Ecol Labs, Provo, UT 84602 USA. [Duncan, Larry; El-Borai, Fahiem E.] Univ Florida, Dept Entomol & Nematol, IFAS, Ctr Citrus Res & Educ, Lake Alfred, FL USA. [El-Borai, Fahiem E.] Zagazig Univ, Dept Plant Protect, Fac Agr, Zagazig, Egypt. [Koppenhoefer, Heather] Univ Florida, Dept Entomol & Nematol, Gainesville, FL 32611 USA. [Tailliez, Patrick] INRA, Divers Genomes & Interact Microorganismes Insectes, F-34060 Montpellier, France. [Tailliez, Patrick] Univ Montpellier 2, Divers Genomes & Interact Microorganismes Insectes, Montpellier, France. RP Shapiro-Ilan, DI (reprint author), USDA ARS, SE Fruit & Tree Nut Res Lab, Byron, GA 31008 USA. EM David.Shapiro@ars.usda.gov RI Adams, Byron/C-3808-2009; OI Adams, Byron/0000-0002-7815-3352; Blackburn, Dana/0000-0001-5153-3366 NR 48 TC 3 Z9 3 U1 2 U2 20 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD DEC PY 2014 VL 46 IS 4 BP 336 EP 345 PG 10 WC Zoology SC Zoology GA CA1CE UT WOS:000348649800004 PM 25580026 ER PT J AU Lu, P Davis, RF Kemerait, RC van Iersel, MW Scherm, H AF Lu, Ping Davis, Richard F. Kemerait, Robert C. van Iersel, Marc W. Scherm, Harald TI Physiological Effects of Meloidogyne incognita Infection on Cotton Genotypes with Differing Levels of Resistance in the Greenhouse SO JOURNAL OF NEMATOLOGY LA English DT Article DE chlorophyll; cotton; Gossypium hirsutum; height-to-node ratio; host-parasitic relationship; Meloidogyne incognita; photosynthesis; physiological stress; southern root-knot nematode ID PHOTOSYNTHESIS; NEMATODES; JAVANICA; STRESS; GROWTH; PLANTS AB Greenhouse tests were conducted to evaluate (i) the effect of Meloidogyne incognita infection in cotton on plant growth and physiology including the height-to-node ratio, chlorophyll content, dark-adapted quantum yield of photosystem II, and leaf area; and (ii) the extent to which moderate or high levels of resistance to M. incognita influenced these effects. Cultivars FiberMax 960 BR (susceptible to M. incognita) and Stoneville 5599 BR (moderately resistant) were tested together in three trials, and PD94042 (germplasm, susceptible) and 120R1B1 (breeding line genetically similar to PD94042, but highly resistant) were paired in two additional trials. Inoculation with M. incognita generally resulted in increases in root gall ratings and egg counts per gram of root compared with the noninoculated control, as well as reductions in plant dry weight, root weight, leaf area, boll number, and boll dry weight, thereby confirming that growth of our greenhouse-grown plants was reduced in the same ways that would be expected in field-grown plants. In all trials, M. incognita caused reductions in height-to-node ratios. Nematode infection consistently reduced the area under the height-to-node ratio curves for all genotypes, and these reductions were similar for resistant and susceptible genotypes (no significant genotype 3 inoculation interaction). Our study is the first to show that infection by M. incognita is associated with reduced chlorophyll content in cotton leaves, and the reduction in the resistant genotypes was similar to that in the susceptible genotypes (no interaction). The susceptible PD94042 tended to have increased leaf temperature compared with the genetically similar but highly resistant 120R1B1 (P < 0.08), likely attributable to increased water stress associated with M. incognita infection. C1 [Lu, Ping; Kemerait, Robert C.] Univ Georgia, Dept Plant Pathol, Tifton, GA 31793 USA. [Davis, Richard F.] USDA ARS, Crop Protect & Management Res Unit, Tifton, GA 31793 USA. [van Iersel, Marc W.] Univ Georgia, Dept Hort, Athens, GA 30602 USA. [Scherm, Harald] Univ Georgia, Dept Plant Pathol, Athens, GA 30602 USA. RP Lu, P (reprint author), Univ Georgia, Dept Plant Pathol, POB 748, Tifton, GA 31793 USA. EM Richard.Davis@ars.usda.gov FU Cotton Incorporated; Georgia Cotton Commission FX Mention of trade names or commercial products is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture or the University of Georgia. Funding for this project was provided in part by Cotton Incorporated and the Georgia Cotton Commission. NR 29 TC 1 Z9 1 U1 1 U2 5 PU SOC NEMATOLOGISTS PI MARCELINE PA PO BOX 311, MARCELINE, MO 64658 USA SN 0022-300X J9 J NEMATOL JI J. Nematol. PD DEC PY 2014 VL 46 IS 4 BP 352 EP 359 PG 8 WC Zoology SC Zoology GA CA1CE UT WOS:000348649800006 PM 25580028 ER PT J AU Ma, Y Tucker, KL Smith, CE Lee, YC Huang, T Richardson, K Parnell, LD Lai, CQ Young, KL Justice, AE Shao, Y North, KE Ordovas, JM AF Ma, Y. Tucker, K. L. Smith, C. E. Lee, Y. C. Huang, T. Richardson, K. Parnell, L. D. Lai, C. Q. Young, K. L. Justice, A. E. Shao, Y. North, K. E. Ordovas, J. M. TI Lipoprotein lipase variants interact with polyunsaturated fatty acids for obesity traits in women: Replication in two populations SO NUTRITION METABOLISM AND CARDIOVASCULAR DISEASES LA English DT Article DE Gene-diet interaction; Lipoprotein lipase; Polyunsaturated fatty acids; Obesity ID FOOD-FREQUENCY QUESTIONNAIRE; BODY-MASS INDEX; ATHEROSCLEROSIS RISK; PHYSICAL-ACTIVITY; SKELETAL-MUSCLE; GENE VARIATION; PUERTO-RICAN; DISEASE; ADULTS; COMMUNITIES AB Background and aims: Lipoprotein lipase (LPL) is a candidate gene for obesity based on its role in triglyceride hydrolysis and the partitioning of fatty acids towards storage or oxidation. Whether dietary fatty acids modify LPL associated obesity risk is unknown. Methods and results: We examined five single nucleotide polymorphisms (SNPs) (rs320, rs2083637, rs17411031, rs13702, rs2197089) for potential interaction with dietary fatty acids for obesity traits in 1171 participants (333 men and 838 women, aged 45-75 y) of the Boston Puerto Rican Health Study (BPRHS). In women, SNP rs320 interacted with dietary polyunsaturated fatty acids (PUFA) for body mass index (BMI) (P = 0.002) and waist circumference (WC) (P = 0.001) respectively. Higher intake of PUFA was associated with lower BMI and WC in homozygotes of the major allele (TT) (P = 0.01 and 0.005) but not in minor allele carriers (TG and GG). These interactions were replicated in an independent population, African American women of the Atherosclerosis Risk in Communities (ARIC) study (n = 1334). Conclusion: Dietary PUFA modulated the association of LPL rs320 with obesity traits in two independent populations. These interactions may be relevant to the dietary management of obesity, particularly in women. (C) 2014 Published by Elsevier B.V. C1 [Ma, Y.; Smith, C. E.; Lee, Y. C.; Richardson, K.; Parnell, L. D.; Lai, C. Q.; Ordovas, J. M.] Tufts Univ, Jean Mayer US Dept Agr Human Nutr Res Ctr, Boston, MA 02111 USA. [Tucker, K. L.] Univ Massachusetts, Lowell, MA USA. [Huang, T.] Harvard Univ, Sch Med, Dept Nutr, Boston, MA USA. [Young, K. L.; Justice, A. E.; Shao, Y.; North, K. E.] Univ N Carolina, Dept Epidemiol, Chapel Hill, NC USA. [Young, K. L.] Univ N Carolina, Carolina Populat Ctr, Chapel Hill, NC USA. [North, K. E.] Univ N Carolina, Carolina Ctr Genome Sci, Chapel Hill, NC USA. [Ordovas, J. M.] CNIC, Dept Epidemiol, Madrid, Spain. [Ordovas, J. M.] Inst Madrileno Estudios Avanzados Alimentac IMDEA, Madrid, Spain. RP Ordovas, JM (reprint author), Tufts Univ, JM USDA Human Nutr Res Ctr Aging, Nutr & Genom Lab, 711 Washington St, Boston, MA 02111 USA. EM jose.ordovas@tufts.edu RI Huang, Tao/C-5641-2014; Young, Kristin/B-5792-2008 OI Huang, Tao/0000-0002-0328-1368; Young, Kristin/0000-0003-0070-6145 FU National Institutes of Health (NIH), National Institute on Aging [P01AG023394]; NIH/National Heart, Lung and Blood Institute [HL54776, P50 HL105185]; NIH/National Institute of Diabetes and Digestive and Kidney Diseases [DK075030, R01 DK075681]; US Department of Agriculture Research Service [53-K06-5-10, 58-1950-9-001]; NIH/Eunice Kennedy Shriver National Institute of Child Health and Human Development [R01 HD057194]; NIH/National Institute of Diabetes And Digestive And Kidney Diseases; National Heart, Lung, and Blood Institute [HHSN268201100005C, HHSN268201100006C, HHSN268 201100007C, HHSN268201100008C, HHSN268201 100009C, HHSN268201100010C, HHSN268201100011C, HHSN268201100012C]; NIDDK [R01 DK089256]; [K08 HL112845-01] FX J.M.O. and C.E.S. contributed to experimental design, results interpretation, manuscript writing and editing. Y.M. contributed to genotyping, data analysis, results interpretation, and manuscript writing. K.L.T. contributed to sample collection of BPRHS, results interpretation, manuscript editing. K.E.N, Y.S., K.L.Y., and A.E.J. contributed to sample collection and data analysis in ARIC, and manuscript editing. Y.C.L. and T.H. contributed to assistance with genotyping in BPRHS, results interpretation and manuscript editing. C.Q.L., L.D.P., and K.R. contributed to results interpretation and manuscript editing. This study was supported by the National Institutes of Health (NIH), National Institute on Aging, grant number P01AG023394 and NIH/National Heart, Lung and Blood Institute, grant number HL54776 and P50 HL105185, and NIH/National Institute of Diabetes and Digestive and Kidney Diseases, grant number DK075030, and contracts 53-K06-5-10 and 58-1950-9-001 from the US Department of Agriculture Research Service, and NIH/Eunice Kennedy Shriver National Institute of Child Health and Human Development, grant number R01 HD057194, and NIH/National Institute of Diabetes And Digestive And Kidney Diseases, grant number R01 DK075681. Caren Smith is supported by K08 HL112845-01. The Atherosclerosis Risk in Communities Study is carried out as a collaborative study supported by National Heart, Lung, and Blood Institute contracts (HHSN268201100005C, HHSN268201100006C, HHSN268 201100007C, HHSN268201100008C, HHSN268201 100009C, HHSN268201100010C, HHSN268201100011C, and HHSN268201100012C). Dr. North is supported by NIDDK R01 DK089256. The authors thank the staff and participants of the ARIC study for their important contributions. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. The USDA is an equal opportunity provider and employer. NR 29 TC 0 Z9 0 U1 1 U2 11 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0939-4753 EI 1590-3729 J9 NUTR METAB CARDIOVAS JI Nutr. Metab. Carbiovasc. Dis. PD DEC PY 2014 VL 24 IS 12 BP 1323 EP 1329 DI 10.1016/j.numecd.2014.07.003 PG 7 WC Cardiac & Cardiovascular Systems; Endocrinology & Metabolism; Nutrition & Dietetics SC Cardiovascular System & Cardiology; Endocrinology & Metabolism; Nutrition & Dietetics GA AZ2CU UT WOS:000348043200009 PM 25156894 ER PT J AU Johnston, R Wang, MH Sun, Q Sylvester, AW Hake, S Scanlon, MJ AF Johnston, Robyn Wang, Minghui Sun, Qi Sylvester, Anne W. Hake, Sarah Scanlon, Michael J. TI Transcriptomic Analyses Indicate That Maize Ligule Development Recapitulates Gene Expression Patterns That Occur during Lateral Organ Initiation SO PLANT CELL LA English DT Article ID POLAR AUXIN TRANSPORT; SHOOT APICAL MERISTEM; RELATIVE CARDAMINE-HIRSUTA; HOMEOBOX GENE; ARABIDOPSIS-THALIANA; LEAF DEVELOPMENT; HOMEODOMAIN PROTEINS; CELL FATE; INFLORESCENCE ARCHITECTURE; ECTOPIC EXPRESSION AB Development of multicellular organisms proceeds via the correct interpretation of positional information to establish boundaries that separate developmental fields with distinct identities. The maize (Zea mays) leaf is an ideal system to study plant morphogenesis as it is subdivided into a proximal sheath and a distal blade, each with distinct developmental patterning. Specialized ligule and auricle structures form at the blade-sheath boundary. The auricles act as a hinge, allowing the leaf blade to project at an angle from the stem, while the ligule comprises an epidermally derived fringe. Recessive liguleless1 mutants lack ligules and auricles and have upright leaves. We used laser microdissection and RNA sequencing to identify genes that are differentially expressed in discrete cell/tissue-specific domains along the proximal-distal axis of wild-type leaf primordia undergoing ligule initiation and compared transcript accumulation in wild-type and liguleless1-R mutant leaf primordia. We identified transcripts that are specifically upregulated at the blade-sheath boundary. A surprising number of these "ligule genes" have also been shown to function during leaf initiation or lateral branching and intersect multiple hormonal signaling pathways. We propose that genetic modules utilized in leaf and/or branch initiation are redeployed to regulate ligule outgrowth from leaf primordia. C1 [Johnston, Robyn; Scanlon, Michael J.] Cornell Univ, Plant Biol Sect, Ithaca, NY 14853 USA. [Wang, Minghui; Sun, Qi] Cornell Univ, Computat Biol Serv Unit, Ithaca, NY 14853 USA. [Sylvester, Anne W.] Univ Wyoming, Dept Dev Genet, Laramie, WY 82071 USA. [Hake, Sarah] Univ Calif Berkeley, Ctr Plant Gene Express, USDA, ARS,Plant & Microbiol Biol Dept, Berkeley, CA 94720 USA. RP Scanlon, MJ (reprint author), Cornell Univ, Plant Biol Sect, Ithaca, NY 14853 USA. EM mjs298@cornell.edu RI Wang, Minghui/G-4939-2015 FU National Science Foundation [MCB 1052051] FX We thank the reviewers for helpful comments that improved the article. We thank J. Moon for images used in Figure 1. We are grateful to S. Leiboff for helpful reviews of the article. We thank X. Rebocho, A. Richardson, and E. Coen for stimulating discussions of maize ligule development. We thank N. R.R. Todt for assistance in performing in situ hybridizations. We thank Mamta Srivastava at the Plant Cell Imaging Center, Boyce Thompson Institute for Plant Research for assistance with confocal microscopy. This study was supported by National Science Foundation Grant MCB 1052051. NR 87 TC 8 Z9 8 U1 2 U2 18 PU AMER SOC PLANT BIOLOGISTS PI ROCKVILLE PA 15501 MONONA DRIVE, ROCKVILLE, MD 20855 USA SN 1040-4651 EI 1532-298X J9 PLANT CELL JI Plant Cell PD DEC PY 2014 VL 26 IS 12 BP 4718 EP 4732 DI 10.1105/tpc.114.132688 PG 15 WC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology SC Biochemistry & Molecular Biology; Plant Sciences; Cell Biology GA CA1AW UT WOS:000348646000015 PM 25516601 ER PT J AU Spackman, E Pantin-Jackwood, MJ AF Spackman, Erica Pantin-Jackwood, Mary J. TI Practical aspects of vaccination of poultry against avian influenza virus SO VETERINARY JOURNAL LA English DT Review DE Avian influenza; Vaccination; Poultry; Domestic fowl; Turkey; Domestic duck ID VACCINES PROTECT CHICKENS; NEWCASTLE-DISEASE VACCINE; MATERNAL ANTIBODY; LOW-PATHOGENICITY; ANTIGENIC CARTOGRAPHY; LIMITED PROTECTION; GENETIC EVOLUTION; NORTH-AMERICA; H5N1 STRAINS; COLD STRESS AB Although little has changed in vaccine technology for avian influenza virus (AIV) in the past 20 years, the approach to vaccination of poultry (chickens, turkeys and ducks) for avian influenza has evolved as highly pathogenic AIV has become endemic in several regions of the world. Vaccination for low pathogenicity AIV is also becoming routine in regions where there is a high level of field challenge. In contrast, some countries will not use vaccination at all and some will only use it on an emergency basis during eradication efforts (i.e. stamping-out). There are pros and cons to each approach and, since every outbreak situation is different, no one method will work equally well in all situations. Numerous practical aspects must be considered when developing an AIV control program with vaccination as a component, such as: (1) the goals of vaccination must be defined; (2) the population to be vaccinated must be clearly identified; (3) there must be a plan to obtain and administer good quality vaccine in a timely manner and to achieve adequate coverage with the available resources; (4) risk factors for vaccine failure should be mitigated as much as possible; and, most importantly, (5) biosecurity must be maintained as much as possible, if not enhanced, during the vaccination period. Published by Elsevier Ltd. C1 [Spackman, Erica; Pantin-Jackwood, Mary J.] USDA ARS, Southeast Poultry Res Lab, Athens, GA 30605 USA. RP Spackman, E (reprint author), USDA ARS, Southeast Poultry Res Lab, 934 Coll Stn Rd, Athens, GA 30605 USA. EM erica.spackman@ars.usda.gov NR 89 TC 2 Z9 3 U1 2 U2 24 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1090-0233 EI 1532-2971 J9 VET J JI Vet. J. PD DEC PY 2014 VL 202 IS 3 BP 408 EP 415 DI 10.1016/j.tvjl.2014.09.017 PG 8 WC Veterinary Sciences SC Veterinary Sciences GA AZ1SG UT WOS:000348017500004 PM 25296849 ER PT J AU Lin, LG Lee, JH Bongmba, OYN Ma, XJ Zhu, XW Sheikh-Hamad, D Sun, YX AF Lin, Ligen Lee, Jong Han Bongmba, Odelia Y. N. Ma, Xiaojun Zhu, Xiongwei Sheikh-Hamad, David Sun, Yuxiang TI The suppression of ghrelin signaling mitigates age-associated thermogenic impairment SO AGING-US LA English DT Article DE ghrelin; growth hormone secretagogue receptor (GHSR); brown adipose tissue (BAT); thermogenesis; insulin signaling; mitochondrial biogenesis and dynamics ID BROWN ADIPOSE-TISSUE; HORMONE SECRETAGOGUE RECEPTOR; IMPROVES INSULIN SENSITIVITY; ACTIVATED PROTEIN-KINASE; GROWTH-HORMONE; FOOD-INTAKE; MITOCHONDRIAL DYNAMICS; GLUCOSE-HOMEOSTASIS; ENERGY-EXPENDITURE; MESSENGER-RNA AB Aging is associated with severe thermogenic impairment, which contributes to obesity and diabetes in aging. We previously reported that ablation of the ghrelin receptor, growth hormone secretagogue receptor (GHS-R), attenuates age-associated obesity and insulin resistance. Ghrelin and obestatin are derived from the same preproghrelin gene. Here we showed that in brown adipocytes, ghrelin decreases the expression of thermogenic regulator but obestatin increases it, thus showing the opposite effects. We also found that during aging, plasma ghrelin and GHS-R expression in brown adipose tissue (BAT) are increased, but plasma obestatin is unchanged. Increased plasma ghrelin and unchanged obestatin during aging may lead to an imbalance of thermogenic regulation, which may in turn exacerbate thermogenic impairment in aging. Moreover, we found that GHS-R ablation activates thermogenic signaling, enhances insulin activation, increases mitochondrial biogenesis, and improves mitochondrial dynamics of BAT. In addition, we detected increased norepinephrine in the circulation, and observed that GHS-R knockdown in brown adipocytes directly stimulates thermogenic activity, suggesting that GHS-R regulates thermogenesis via both central and peripheral mechanisms. Collectively, our studies demonstrate that ghrelin signaling is an important thermogenic regulator in aging. Antagonists of GHS-R may serve as unique anti-obesity agents, combating obesity by activating thermogenesis. C1 [Lin, Ligen; Lee, Jong Han; Bongmba, Odelia Y. N.; Sun, Yuxiang] Baylor Univ, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Lin, Ligen] Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China. [Ma, Xiaojun] Zhengzhou Univ, Affiliated Hosp 1, Dept Endocrinol, Zhengzhou 450052, Peoples R China. [Zhu, Xiongwei] Case Western Reserve Univ, Dept Pathol, Cleveland, OH 44106 USA. [Sheikh-Hamad, David; Sun, Yuxiang] Baylor Coll Med, Dept Med, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Huffington Ctr Aging, Houston, TX 77030 USA. [Sun, Yuxiang] Baylor Coll Med, Dept Mol & Cellular Biol, Houston, TX 77030 USA. RP Sun, YX (reprint author), Baylor Univ, Dept Pediat, USDA ARS, Childrens Nutr Res Ctr, Houston, TX 77030 USA. EM yuxiangs@bcm.edu FU USDA/CRIS [ARS 6250-51000-059] FX Indirect calorimetry was performed in the Mouse Metabolic Research Unit at the Children's Nutrition Research Center, Baylor College of Medicine. The authors acknowledge the expert assistance of Mr. Firoz Vohra and the MMRU Core Director Dr. Marta Fiorotto. We also thank Mr. Michael R. Honig at Houston's Community Public Radio Station KPFT for his excellent editorial assistance. This study was supported by USDA/CRIS grant ARS 6250-51000-059 (YS), American Heart Association (AHA) innovative grant 12IRG9230004 (YS) and Grant-in-Aid 14GRNT18990019 (YS), NIH RDK080306 (DSH), VA BX002006 (DSH), NIH training grant 1T32HD071839 (PI: Morey W. Haymond), and partly supported by the NIH-Diabetes and Endocrinology Research Center of Baylor College of Medicine (P30DK079638). This study was also supported by Macao Science and Technology Development Fund 042/2013/A2 (LL) and the Research Fund of University of Macau MRG015/LLG/2014/ICMS (LL). NR 76 TC 9 Z9 9 U1 1 U2 3 PU IMPACT JOURNALS LLC PI ALBANY PA 6211 TIPTON HOUSE, STE 6, ALBANY, NY 12203 USA SN 1945-4589 J9 AGING-US JI Aging-US PD DEC PY 2014 VL 6 IS 12 BP 1019 EP 1032 PG 14 WC Cell Biology SC Cell Biology GA AZ2HR UT WOS:000348055500003 PM 25543537 ER PT J AU Timilsina, N Escobedo, FJ Staudhammer, CL Brandeis, T AF Timilsina, Nilesh Escobedo, Francisco J. Staudhammer, Christina L. Brandeis, Thomas TI Analyzing the causal factors of carbon stores in a subtropical urban forest SO ECOLOGICAL COMPLEXITY LA English DT Article DE Carbon storage; Path analysis; Urban forest biomass; Puerto Rico; Urban forest structure ID PLANT-SPECIES RICHNESS; UNITED-STATES; ABOVEGROUND BIOMASS; ECOSYSTEM FUNCTION; TREE COVER; STORAGE; BIODIVERSITY; STOCKS; SEQUESTRATION; DIVERSITY AB Studies of forests and urban forest ecosystems have documented the various biophysical and socioeconomic correlates of carbon storage. Tree cover in particular is often used as a determinant of carbon storage for local and national level urban forest assessments. However, the relationships among variables describing the biophysical and socioeconomic environment and carbon are not simple statistical ones. Instead, there are complex interactions that can have either a unidirectional causal effects, or produce indirect effects through interactions with other ecosystem structure and landscape characteristics. Thus, understanding the direct and indirect effects of structure, composition, and landscape characteristics is key to quantifying ecosystem services. This study used field data from plots across an urban watershed, site-specific biomass equations, and structural equation modeling of urban forest structure and landscape variables to quantify the causal influences of tree cover, land use, stand density, species composition and diversity on carbon stores. Our path analysis shows that the effect of tree cover on carbon stores is not only direct but also indirect and influential through basal area and composition. Findings suggest that species composition, species diversity and land use have much more complex relationships than previously reported in the urban forest literature. The use of path analysis in these types of studies also presents a novel method to better analyze and quantify these direct and indirect effects on urban forest carbon stores. Findings have implications for urban forest ecosystem assessments that use tree cover as the sole metric for inferring ecosystem functions and services. (C) 2014 Elsevier B.V. All rights reserved. C1 [Timilsina, Nilesh] Univ Wisconsin, Coll Nat Resources, Stevens Point, WI 54481 USA. [Escobedo, Francisco J.] Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32611 USA. [Staudhammer, Christina L.] Univ Alabama, Dept Biol Sci, Tuscaloosa, AL 35487 USA. [Brandeis, Thomas] USDA, Forest Serv, Southern Res, Knoxville, TN USA. RP Timilsina, N (reprint author), Univ Wisconsin, Coll Nat Resources, 800 Reserve St, Stevens Point, WI 54481 USA. EM ntimilsi@uwsp.edu; fescobed@ufl.edu; cstaudhammer@ua.edu; tjbrandeis@fs.fed.us RI Escobedo, Francisco /H-1286-2016 OI Escobedo, Francisco /0000-0002-9272-5046 FU USDA Tropical and Subtropical Agricultural Research Program (TSTAR-C FY) FX This research was funded by a grant from the USDA Tropical and Subtropical Agricultural Research Program (TSTAR-C FY2008). The authors would like to thank Robin Morgan, Terry Hoffman, Olga Ramos, Eileen Helmer, Jeffrey Glogiewicz and Edgardo Gonzalez in Puerto Rico for field logistics and data assistance. NR 64 TC 6 Z9 7 U1 2 U2 47 PU ELSEVIER SCIENCE BV PI AMSTERDAM PA PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS SN 1476-945X EI 1476-9840 J9 ECOL COMPLEX JI Ecol. Complex. PD DEC PY 2014 VL 20 SI SI BP 23 EP 32 DI 10.1016/j.ecocom.2014.07.001 PG 10 WC Ecology SC Environmental Sciences & Ecology GA AZ1PO UT WOS:000348010800003 ER PT J AU Yadavalli, A Jones, K AF Yadavalli, Anita Jones, Keithly TI Does media influence consumer demand? The case of lean finely textured beef in the United States SO FOOD POLICY LA English DT Article DE LFTB; Meat demand; Switching regime; Media effect; Food scares; Consumer concerns ID US MEAT DEMAND; STRUCTURAL-CHANGE; INFORMATION; IMPACT; FOOD; SYSTEM; PROMOTION; PRODUCTS; INDUSTRY; QUALITY AB In 2012, the media paid extra attention to lean finely textured beef (LFTB), an inexpensive lean beef product extracted from low-valued fatty trim. The media's negative portrayal of LFTB as an unnecessary and unsavory additive to ground beef products corresponded with a spike in reports over a 6-month period. Since LFTB is often used in ground beef, it is likely that consumption of LFTB-based ground beef products and other meats could be affected. This paper used weekly meat production and sales data to assess how media depictions of LFTB affect consumer demand. We used a Central Bureau of Statistics model to test whether media portrayal affects the consumption of aggregate meats and beef cuts: pork, chicken, turkey, Choice beef, Prime beef, Select beef and ground beef. Results indicate that media portrayal of LFTB, measured by the number of articles on the subject weighted by consumer readership, did not lead to significant changes in consumer demand across meats or within the beef category immediately. However, consumer purchases of pork, turkey and Prime beef were affected two weeks or greater after news reports of LFTB surfaced. Nevertheless, those effects were temporary and waned or disappeared during later weeks. Our look at the LFTB controversy informs food policy by studying the media sources from which consumers obtain their information. As the temporary effects of news media on consumer demand for aggregate meats and disaggregate beef cuts suggest, consumers may benefit from receiving their news from industry and government sources instead. Published by Elsevier Ltd. C1 [Yadavalli, Anita; Jones, Keithly] Econ Res Serv, USDA, Washington, DC USA. [Yadavalli, Anita] Purdue Univ, W Lafayette, IN 47907 USA. RP Yadavalli, A (reprint author), 6744 Creek Vale Way Apt 2D, Indianapolis, IN 46237 USA. EM yadavalli.anita@gmail.com NR 65 TC 6 Z9 6 U1 2 U2 22 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 0306-9192 EI 1873-5657 J9 FOOD POLICY JI Food Policy PD DEC PY 2014 VL 49 BP 219 EP 227 DI 10.1016/j.foodpol.2014.08.002 PN 1 PG 9 WC Agricultural Economics & Policy; Economics; Food Science & Technology; Nutrition & Dietetics SC Agriculture; Business & Economics; Food Science & Technology; Nutrition & Dietetics GA AY7SZ UT WOS:000347760400022 ER PT J AU Park, SA Grusak, MA Oh, MM AF Park, Sin-Ae Grusak, Michael A. Oh, Myung-Min TI Concentrations of Minerals and Phenolic Compounds in Three Edible Sprout Species Treated with Iron-chelates during Imbibition SO HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY LA English DT Article DE alfalfa; broccoli; Fe(III)-citrate; Fe(III)-EDTA; radish; total phenolic concentration ID BROCCOLI SPROUTS; SEED; PLANTS; HEALTH; PHYTOCHEMICALS; PERMEABILITY; PREVENTION; NUTRITION; BIOLOGY; OXYGEN AB Iron (Fe) is an essential micronutrient involved in fundamental biological processes in both humans and plants. Iron deficiency is common in humans, making iron supplementation of foods an important area of research. Edible sprouts are a rich source of minerals and phenolic compounds beneficial to human health; our objective was therefore to investigate the effects of iron supplementation in sprouts. We supplemented iron concentrations in three species of edible sprouts (alfalfa, broccoli, and radish) by soaking the seeds in a high-iron solution, and subsequently measured the concentration of minerals and of phenolic compounds. Seeds were soaked in either Fe(III)-EDTA or Fe(III)-citrate at concentrations of 2.5, 5.0, or 10 mM for 5-8 h, and then were maintained with distilled water in a commercial sprouter for 5 days. The soaking treatment significantly increased the iron concentration in 5-day-old alfalfa sprouts by up to 1.8 times the concentration observed in the controls. For broccoli and radish sprouts, an insignificant trend toward higher Fe concentrations was observed. The accumulated iron in treated alfalfa sprouts was negatively associated with concentrations of other minerals such as Ca, Mg, Mn, and Na. Treated alfalfa sprouts showed a significant increase of 8.0-36.4% in total phenolic concentrations compared to the controls, whereas broccoli and radish sprouts showed no significant change in phenolic concentrations. In summary, soaking seeds with iron chelates enhanced the iron concentration of sprouts, especially alfalfa sprouts, and had a positive or neutral impact on the concentration of phenolic compounds, suggesting that this treatment could be used to improve the nutritional quality of some types of edible sprouts. C1 [Park, Sin-Ae] Konkuk Univ, Dept Environm Hlth Sci, Seoul 143701, South Korea. [Grusak, Michael A.] Baylor Coll Med, Dept Pediat, USDA ARS Childrens Nutr Res Ctr, Houston, TX 77030 USA. [Oh, Myung-Min] Chungbuk Natl Univ, Div Anim Hort & Food Sci, Cheongju 361763, South Korea. [Oh, Myung-Min] Chungbuk Natl Univ, Brain Korea Ctr Bioresource Dev 21, Cheongju 361763, South Korea. RP Oh, MM (reprint author), Chungbuk Natl Univ, Div Anim Hort & Food Sci, Cheongju 361763, South Korea. EM moh@cbnu.ac.kr FU SMART Research Professor Program of Konkuk University; USDA-ARS [58-6250-0-008] FX This study was supported by the SMART Research Professor Program of Konkuk University. MAG was also supported in part by funds from USDA-ARS Agreement 58-6250-0-008. NR 38 TC 3 Z9 3 U1 0 U2 6 PU KOREAN SOC HORTICULTURAL SCIENCE PI SUWON PA C/O NATL HORTICULTURAL RES INST, IMOK-DONG 475, JANGAN-GU, SUWON, 440-706, SOUTH KOREA SN 2211-3452 EI 2211-3460 J9 HORTIC ENVIRON BIOTE JI Hortic. Environ. Biotechnol. PD DEC PY 2014 VL 55 IS 6 BP 471 EP 478 DI 10.1007/s13580-014-0075-9 PG 8 WC Horticulture SC Agriculture GA AZ0TL UT WOS:000347957700005 ER PT J AU Jin, TZ Guo, MM Yang, RJ AF Jin, Tony Z. Guo, Mingming Yang, Ruijin TI Combination of pulsed electric field processing and antimicrobial bottle for extending microbiological shelf-life of pomegranate juice SO INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES LA English DT Article DE Pulsed eclectic fields; Antimicrobial packaging; Bottle; Shelf-life; Pomegranate juice; Hurdle technology ID ESCHERICHIA-COLI O157-H7; FOOD PRESERVATION; POLYLACTIC ACID; APPLE CIDER; SALMONELLA-TYPHIMURIUM; LISTERIA-MONOCYTOGENES; ANTIOXIDANT CAPACITY; ORANGE JUICE; FRUIT JUICES; INACTIVATION AB Pomegranate juice was processed using bench top (7.2 L/h flow rate, 35 kV/cm field strength, 72 mu s total treatment time) and pilot scale (100 L/h flow rate, 35 kV/cm field strength, 281 mu s total treatment time) continuous pulsed electric field (PEF) processing systems. The treated juice was packaged in PET bottles or PET bottles coated with potassium sorbate and sodium benzoate, and stored at 4 degrees C for 84 days. Samples were assessed every 7 days for total aerobic bacteria and yeast and mold. Untreated juice had less than one week of shelf-life, while untreated juices in antimicrobial bottles had 56 days. Juices treated with PEF alone had a shelf-life of 21 days (bench scale) and over 84 days (pilot scale). juices treated with PEF and stored in antimicrobial bottles had a shelf-life over 84 days for both scale tests, which significantly extended the microbiological shelf-life of pomegranate juice. Industrial relevance: Pulsed electric field (PEF), one of novel non-thermal processing technologies, has been studied intensively worldwide for the last decades. However, most of them were done at laboratory scale and few were at pilot or commercial scale. In addition, PEF processing alone may not provide enough shelf-life of juice as juice industry expects. The work in this paper shows the side-by-side comparison of PEF processing at lab and pilot scales and demonstrates that the combination of PEF with antimicrobial battle packaging significantly extended the shelf-life of juice. The use of a large scale PEF processing system and the combination of antimicrobial packaging provide juice manufacturers an innovate approach for enhancing the safety and extending the shelf-life of juice products. Published by Elsevier Ltd. C1 [Jin, Tony Z.] ARS, USDA, Eastern Reg Res Ctr, Wyndmoor, PA 19038 USA. [Guo, Mingming; Yang, Ruijin] Jiangnan Univ, Wuxi 214122, Jiangsu, Peoples R China. RP Jin, TZ (reprint author), ARS, USDA, Eastern Reg Res Ctr, 600 East Mermaid Lane, Wyndmoor, PA 19038 USA. EM tony.jin@ars.usda.gov OI Jin, Tony/0000-0003-0504-5817 FU AMC Group, Spain under the Cooperative Research and Development Agreement [58-3K95-2-1543] FX This project was in collaboration with AMC Group, Spain under the Cooperative Research and Development Agreement (# 58-3K95-2-1543). The authors thank Anita Parameswaran for excellent technical laboratory assistance, Andy Bigley for PEF pilot system operation, and Dr. Brendan Niemira for reviewing this manuscript and providing helpful feedback. NR 36 TC 2 Z9 2 U1 3 U2 33 PU ELSEVIER SCI LTD PI OXFORD PA THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND SN 1466-8564 EI 1878-5522 J9 INNOV FOOD SCI EMERG JI Innov. Food Sci. Emerg. Technol. PD DEC PY 2014 VL 26 BP 153 EP 158 DI 10.1016/j.ifset.2014.07.011 PG 6 WC Food Science & Technology SC Food Science & Technology GA AY4ZC UT WOS:000347582400019 ER PT J AU Aziz, I Mahmood, T Islam, KR AF Aziz, Irfan Mahmood, Tariq Islam, Khandakar Rafiq TI IMPACT OF SOLE CROPPING AND MULTIPLE CROPPING ON SOIL HUMIFIED CARBON FRACTIONS SO PAKISTAN JOURNAL OF BOTANY LA English DT Article DE Sole Cropping and Multiple Cropping; Total Humified Carbon; Humin; Humic Acid; Fulvic acid ID ORGANIC-MATTER; HUMIC FRACTIONS; STRUCTURAL STABILITY; MICROBIAL BIOMASS; MANAGEMENT; DYNAMICS; TILLAGE; MAIZE AB The present study was planned to improve our understanding how crop rotation can enhance humified C fractions. A long term experiment was conducted on Vanmeter farm of the Ohio State University South Centers at Piketon Ohio, USA from 2002 to 2007. Crop rotation treatments included were continuous corn (CC), corn-soybean (CS) and corn-soybean-wheat-cowpea (CSW) rotations. Randomized complete block design with 6 replications was used under natural field conditions. The findings of this long-term study revealed that multiple cropping had significantly improved humified carbon fractions compared to mono-cropping system. Although total humified carbon (THOC), sugar free humified carbon (HOC) concentration were non-significant however, humin (NH) contents, humic (HA), fulvic acids (FA), humic and fulvic acid associated glucose (HA-NH and FA-NH) were significantly affected by various crop rotations within five years. The soil under CC had 22-52% significantly greater NH concentration than CSW and CS rotations respectively. Similarly all crop rotations had shown 5-16 increase in HA and 5-17% decreased in FA over time. Likewise soil under CC had 16 and 54% greater HA-NH concentration as compared to CSW and CS rotations. The FA-NH concentration increased significantly by 27-51% in soil under all treatments over time. The soil under CSW had greater HA/FA (1.6) fallowed by CC (1.4) and CS (1.1). Soils under CSW had significantly greater HA/HOC (12-18%) as compare to CC and CS respectively. Conversely, the value of FA/HOC decreased (1-23%) in soil under all crop rotation treatments within five years. Degree of humification (DH) had shown a significant increase (7-12%) in soil under all treatments as compared to 2002. Irrespective of crop rotation THOC, HOC, NH, humin, HA, HR and FA/HOC concentration decreased significantly with increase in soil depth. While fulvic acid concentration HA/HOC in all crop rotation increased with increase in soil depth. The effect of crop rotation on humified C fractions could be because of variations in type, amount and quality of C returned by different plants into the soil. So replacing mono-cropping with multiple cropping can enhance humified C fractions and can improve soil functional properties. C1 [Aziz, Irfan] PMS Arid Agr Univ, Dept Agron, Rawalpindi, Pakistan. [Mahmood, Tariq] PMS Arid Agr Univ, Dept Environm Sci, Rawalpindi, Pakistan. [Islam, Khandakar Rafiq] Ohio State Univ, South Ctr, Piketon, OH USA. [Islam, Khandakar Rafiq] ARS, USDA, Columbus, OH USA. RP Mahmood, T (reprint author), PMS Arid Agr Univ, Dept Environm Sci, Rawalpindi, Pakistan. EM tamahmood@hec.gov.pk NR 30 TC 1 Z9 1 U1 0 U2 2 PU PAKISTAN BOTANICAL SOC PI KARACHI PA DEPT OF BOTANY UNIV KARACHI, 32 KARACHI, PAKISTAN SN 0556-3321 EI 2070-3368 J9 PAK J BOT JI Pak. J. Bot. PD DEC PY 2014 VL 46 IS 6 BP 2157 EP 2162 PG 6 WC Plant Sciences SC Plant Sciences GA AY5QS UT WOS:000347627500032 ER PT J AU Goolsby, JA Gaskin, JF Tarin, DV Pepper, AE Henne, DC Auclair, A Racelis, AE Summy, KR Moran, PJ Thomas, DB Yang, CH Jimenez, MM Ciomperlik, MJ de Leon, AAP Kirk, AA AF Goolsby, John A. Gaskin, John F. Tarin, Daniel V. Pepper, Alan E. Henne, Don C. Auclair, Allan Racelis, Alexis E. Summy, Kenneth R. Moran, Patrick J. Thomas, Donald B. Yang, Chenghai Martinez Jimenez, Maricela Ciomperlik, Matthew J. de Leon, Adalberto A. Perez Kirk, Alan A. TI Establishment and Spread of a Single Parthenogenic Genotype of the Mediterranean arundo wasp, Tetramesa romana(1), In the Variable Climate of Texas SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID BIOLOGICAL-CONTROL AGENT; AMPLEXICAULIS VAR. BARBARAE; GIANT REED; HYMENOPTERA EURYTOMIDAE; NORTH-AMERICA; RIO-GRANDE; DONAX; BRASSICACEAE; IMAGERY AB As part of a biological control program for the invasive weed, Arundo donax L., several genotypically unique populations of the parthenogenetic stem-galling wasp, Tetramesa romana Walker (Hymenoptera: Eurytomidae), from Spain and France were released in an infested riparian zone along the Rio Grande from Brownsville to Del Rio, TX. An adventive population of the wasp of unknown origin with limited distribution in Texas was also discovered, evaluated, and released as part of the program. More than 1.2 million wasps representing the mixture of genotypes were aerially released from 2009 to 2011. Wasps dispersed from their original release locations and now have a continuous distribution along the Rio Grande from Brownsville to Del Rio, and have dispersed throughout most of Central Texas with satellite populations as far west as San Angelo (Tom Green County), north as far as Kaufman (Kaufman County), and east to Navasota (Grimes County). The most successful genotype (#4) represented 390 of the 409 wasps recovered and matched both an imported population from the Mediterranean coast of Spain and an adventive population established in Texas before the start of the biological control program. Several other European genotypes of the wasp released in the program apparently failed to establish. This result demonstrated the benefits of evaluating and releasing the maximum genetic diversity of the biological control agent in the introduced range. Abundance of T. romana on the Rio Grande from Laredo to Del Rio averaged 190% more in 2013-2014 compared to a similar study in 2008-2009 before release of the European wasps. A favorability index was developed that showed that conditions from 1969 to 1977 would have been adverse to the wasp; conditions after 2009 were more favorable. Climate matching predicts the wasp will disperse throughout the southern U. S. and Mexico. C1 [Goolsby, John A.; Thomas, Donald B.] ARS, USDA, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. [Gaskin, John F.] USDA ARS, Sidney, MT USA. [Tarin, Daniel V.; Pepper, Alan E.] Texas A&M Univ, Dept Biol, College Stn, TX 77843 USA. [Henne, Don C.] Texas A&M Univ AgriLife Res, Weslaco, TX USA. [Auclair, Allan] USDA APHIS, Riverdale, MD USA. [Racelis, Alexis E.; Summy, Kenneth R.] Univ Texas Rio Grande Valley, Dept Biol, Edinburg, TX USA. [Moran, Patrick J.] USDA ARS, Exot & Invas Weeds Res Unit, Albany, CA USA. [Yang, Chenghai] USDA ARS, Areawide Pest Management Res Unit, College Stn, TX USA. [Martinez Jimenez, Maricela] Inst Mexicano Tecnol Agua, Jiutepec, Morelos, Mexico. [Ciomperlik, Matthew J.] USDA APHIS PPQ CPHST PDDML, Edinburg, TX USA. [de Leon, Adalberto A. Perez] USDA ARS, Knipling Bushland US Livestock Insects Res Lab, Kerrville, TX USA. [Kirk, Alan A.] USDA ARS, European Biol Control Lab, Montpellier, France. RP Goolsby, JA (reprint author), ARS, USDA, Cattle Fever Tick Res Lab, Edinburg, TX 78541 USA. EM john.goolsby@ars.usda.gov FU Department of Homeland Security; Lower Rio Grande Valley Development Council FX The authors wish to thank Matthew Rector (Texas A&M University Agrilife Research, Weslaco, TX), Emmett Kunz (University of Texas - Pan American, Edinburg, TX), and Reyes Garcia, III (USDA-ARS, Cattle Fever Tick Research Laboratory, Edinburg, TX) for the field collections, and Rupert Santos, Andrew Parker, and Jose Escamilla for mass rearing the wasps. Additional funds for research support were provided by the Department of Homeland Security, and the Lower Rio Grande Valley Development Council. We wish to thank Bill Palmer (Biosecurity Queensland, Department of Agriculture, Fisheries and Forestry, Brisbane, Australia) and Bill Overholt (University of Florida, Ft. Pierce, FL) for reviews of a draft of the manuscript. NR 28 TC 3 Z9 3 U1 6 U2 22 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD DEC PY 2014 VL 39 IS 4 BP 675 EP 689 DI 10.3958/059.039.0414 PG 15 WC Entomology SC Entomology GA AZ2HY UT WOS:000348056100002 ER PT J AU Schwartz, HF Gent, DH Fichtner, SM Otto, K Boateng, CO Szostek, S Cranshaw, WS Mahaffey, LA AF Schwartz, H. F. Gent, D. H. Fichtner, S. M. Otto, K. Boateng, C. O. Szostek, S. Cranshaw, W. S. Mahaffey, L. A. TI Thrips tabaci (Thysanoptera: Thripidae) and Iris yellow spot virus Associated with Onion Transplants, Onion Volunteers, and Weeds in Colorado SO SOUTHWESTERN ENTOMOLOGIST LA English DT Article ID NEW-YORK; HOSTS AB Infestation by onion thrips, Thrips tabaci Lindeman, was determined on transplants of onion (Allium cepa L.) received in Colorado during March and April from out-of-state sources (Imperial Valley, CA; near Phoenix, AZ; and southern Texas) during 2004 to 2008. In the 5 years of the study, 50 to 100% of the transplant lots sampled arrived infested with thrips. Among infested transplant lots, the overall number of thrips averaged 0.15 to 0.63 per plant, with as many as four per plant in some lots. T. tabaci was the dominant thrips species in all seasons and locations of transplant origin. In addition, 19 of 83 (23%) tested lots had plants positive for Iris yellow spot virus. Iris yellow spot virus and T. tabaci were detected in volunteer onion plants as early as 1 May, a few weeks after the summer onion crop was planted, suggesting a possible role of infected volunteer plants in perennation of the virus between onion crops. Iris yellow spot virus and T. tabaci were detected in many common weeds including blue mustard (Chorispora tenella (Pall.) DC), common purslane (Portulaca oleracea L.), field bindweed (Convolvulus arvensis L.), flixweed (Descurainia sophia Webb & Berth.), prickly lettuce (Lactuca serriola L.), and redroot pigweed (Amaranthus retroflexus L.) in early spring near onion fields in Colorado during 2006 to 2009. Confirmation that Iris yellow spot virus and Iris yellow spot virus-infective thrips overwintered in volunteer onions and some common winter annual and perennial weeds emphasizes that managing volunteer onions and weeds is important for management of iris yellow spot, in addition to planting transplants free of thrips and the pathogen. C1 [Schwartz, H. F.; Otto, K.; Boateng, C. O.; Szostek, S.; Cranshaw, W. S.; Mahaffey, L. A.] Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. [Gent, D. H.] ARS, USDA, Corvallis, OR USA. [Gent, D. H.] Oregon State Univ, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA. [Fichtner, S. M.] Western Farm Serv, Fresno, CA USA. RP Schwartz, HF (reprint author), Colorado State Univ, Dept Bioagr Sci & Pest Management, Ft Collins, CO 80523 USA. FU Colorado Onion Association; Colorado State University Agricultural Experiment Station; USDA/CSREES Pest Management Alternatives Program [2006-02954, 2006-34381-16961] FX We wish to thank and acknowledge those assisting in the research of this project: Matthew Camper, Jillian Lang, Lisanne Rude, Bennett Colvin, Seth Klein, Garrett Grossmann, and Mindy Howard. Supporting funds for this study were received from the Colorado Onion Association, Colorado State University Agricultural Experiment Station, and USDA/CSREES Pest Management Alternatives Program grants 2006-02954 and 2006-34381-16961, entitled "Thrips and Iris yellow spot virus Disease Dynamics in Western U.S. Onions". NR 32 TC 1 Z9 1 U1 2 U2 9 PU SOUTHWESTERN ENTOMOLOGICAL SOC PI DALLAS PA 17360 COIT RD, DALLAS, TX 75252-6599 USA SN 0147-1724 EI 2162-2647 J9 SOUTHWEST ENTOMOL JI Southw. Entomol. PD DEC PY 2014 VL 39 IS 4 BP 691 EP 704 DI 10.3958/059.039.0401 PG 14 WC Entomology SC Entomology GA AZ2HY UT WOS:000348056100003 ER PT J AU Luce, CH Lopez-Burgos, V Holden, Z AF Luce, Charles H. Lopez-Burgos, Viviana Holden, Zachary TI Sensitivity of snowpack storage to precipitation and temperature using spatial and temporal analog models SO WATER RESOURCES RESEARCH LA English DT Article ID WESTERN UNITED-STATES; CLIMATE-CHANGE IMPACTS; MOUNTAIN SNOWPACK; NORTH-AMERICA; OROGRAPHIC PRECIPITATION; PACIFIC-NORTHWEST; STREAMFLOW; TRENDS; WATER; DISTRIBUTIONS AB Empirical sensitivity analyses are important for evaluation of the effects of a changing climate on water resources and ecosystems. Although mechanistic models are commonly applied for evaluation of climate effects for snowmelt, empirical relationships provide a first-order validation of the various postulates required for their implementation. Previous studies of empirical sensitivity for April 1 snow water equivalent (SWE) in the western United States were developed by regressing interannual variations in SWE to winter precipitation and temperature. This offers a temporal analog for climate change, positing that a warmer future looks like warmer years. Spatial analogs are used to hypothesize that a warmer future may look like warmer places, and are frequently applied alternatives for complex processes, or states/metrics that show little interannual variability (e.g., forest cover). We contrast spatial and temporal analogs for sensitivity of April 1 SWE and the mean residence time of snow (SRT) using data from 524 Snowpack Telemetry (SNOTEL) stations across the western U.S. We built relatively strong models using spatial analogs to relate temperature and precipitation climatology to snowpack climatology (April 1 SWE, R-2=0.87, and SRT, R-2=0.81). Although the poorest temporal analog relationships were in areas showing the highest sensitivity to warming, spatial analog models showed consistent performance throughout the range of temperature and precipitation. Generally, slopes from the spatial relationships showed greater thermal sensitivity than the temporal analogs, and high elevation stations showed greater vulnerability using a spatial analog than shown in previous modeling and sensitivity studies. The spatial analog models provide a simple perspective to evaluate potential futures and may be useful in further evaluation of snowpack with warming. C1 [Luce, Charles H.; Lopez-Burgos, Viviana] US Forest Serv Res & Dev, Rocky Mt Res Stn, Boise, ID 83702 USA. [Holden, Zachary] US Forest Serv Reg 1, Missoula, MT USA. RP Luce, CH (reprint author), US Forest Serv Res & Dev, Rocky Mt Res Stn, Boise, ID 83702 USA. EM cluce@fs.fed.us RI Luce, Charles/A-9267-2008 OI Luce, Charles/0000-0002-6938-9662 FU NASA [NNH11ZDA001N-FIRES] FX The authors thank Region 1 of the US Forest Service, Department of Agriculture for salary support for Viviana Lopez-Burgos. This work was partially supported by NASA through a NNH11ZDA001N-FIRES award. We thank the USDA Natural Resources Conservation Service for access to the SNOTEL data (http://www.wcc.nrcs.usda.gov/snow/). We also thank Jessica Lundquist and four anonymous reviewers for comments improving the manuscript. NR 50 TC 8 Z9 8 U1 4 U2 23 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD DEC PY 2014 VL 50 IS 12 BP 9447 EP 9462 DI 10.1002/2013WR014844 PG 16 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AZ0FH UT WOS:000347921100020 ER PT J AU Skaggs, TH Anderson, RG Corwin, DL Suarez, DL AF Skaggs, T. H. Anderson, R. G. Corwin, D. L. Suarez, D. L. TI Analytical steady-state solutions for water-limited cropping systems using saline irrigation water SO WATER RESOURCES RESEARCH LA English DT Article ID ROOT-ZONE; LEACHING FRACTION; MANAGEMENT; MODEL; PLANT; SOIL; TOLERANCE; TRANSPORT; DRAINAGE; STRESS AB Due to the diminishing availability of good quality water for irrigation, it is increasingly important that irrigation and salinity management tools be able to target submaximal crop yields and support the use of marginal quality waters. In this work, we present a steady-state irrigated systems modeling framework that accounts for reduced plant water uptake due to root zone salinity. Two explicit, closed-form analytical solutions for the root zone solute concentration profile are obtained, corresponding to two alternative functional forms of the uptake reduction function. The solutions express a general relationship between irrigation water salinity, irrigation rate, crop salt tolerance, crop transpiration, and (using standard approximations) crop yield. Example applications are illustrated, including the calculation of irrigation requirements for obtaining targeted submaximal yields, and the generation of crop-water production functions for varying irrigation waters, irrigation rates, and crops. Model predictions are shown to be mostly consistent with existing models and available experimental data. Yet the new solutions possess advantages over available alternatives, including: (i) the solutions were derived from a complete physical-mathematical description of the system, rather than based on an ad hoc formulation; (ii) the analytical solutions are explicit and can be evaluated without iterative techniques; (iii) the solutions permit consideration of two common functional forms of salinity induced reductions in crop water uptake, rather than being tied to one particular representation; and (iv) the utilized modeling framework is compatible with leading transient-state numerical models. C1 [Skaggs, T. H.; Anderson, R. G.; Corwin, D. L.; Suarez, D. L.] USDA ARS, US Salin Lab, Riverside, CA 92501 USA. RP Skaggs, TH (reprint author), USDA ARS, US Salin Lab, 4500 Glenwood Dr, Riverside, CA 92501 USA. EM todd.skaggs@ars.usda.gov OI Anderson, Ray/0000-0002-6202-5890 NR 45 TC 4 Z9 4 U1 4 U2 14 PU AMER GEOPHYSICAL UNION PI WASHINGTON PA 2000 FLORIDA AVE NW, WASHINGTON, DC 20009 USA SN 0043-1397 EI 1944-7973 J9 WATER RESOUR RES JI Water Resour. Res. PD DEC PY 2014 VL 50 IS 12 BP 9656 EP 9674 DI 10.1002/2014WR016058 PG 19 WC Environmental Sciences; Limnology; Water Resources SC Environmental Sciences & Ecology; Marine & Freshwater Biology; Water Resources GA AZ0FH UT WOS:000347921100031 ER PT J AU Haegerich, TM Paulozzi, LJ Manns, BJ Jones, CM AF Haegerich, Tamara M. Paulozzi, Leonard J. Manns, Brian J. Jones, Christopher M. TI What we know, and don't know, about the impact of state policy and systems-level interventions on prescription drug overdose SO DRUG AND ALCOHOL DEPENDENCE LA English DT Review DE Prescribing; Opioids; Overdose; Policy; Evaluation; Pain ID NALOXONE DISTRIBUTION PROGRAMS; CHRONIC NONCANCER PAIN; NEW-YORK-CITY; OPIOID-OVERDOSE; UNITED-STATES; BENZODIAZEPINE PRESCRIPTION; INTRANASAL NALOXONE; MONITORING PROGRAMS; CLINICAL GUIDELINES; WASHINGTON-STATE AB Background: Drug overdose deaths have been rising since the early 1990s and is the leading cause of injury death in the United States. Overdose from prescription opioids constitutes a large proportion of this burden. State policy and systems-level interventions have the potential to impact prescription drug misuse and overdose. Methods: We searched the literature to identify evaluations of state policy or systems-level interventions using non-comparative, cross-sectional, before-after, time series, cohort, or comparison group designs or randomized/non-randomized trials. Eligible studies examined intervention effects on provider behavior, patient behavior, and health outcomes. Results: Overall study quality is low, with a limited number of time-series or experimental designs. Knowledge and prescribing practices were measured more often than health outcomes (e.g., overdoses). Limitations include lack of baseline data and comparison groups, inadequate statistical testing, small sample sizes, self-reported outcomes, and short-term follow-up. Strategies that reduce inappropriate prescribing and use of multiple providers and focus on overdose response, such as prescription drug monitoring programs, insurer strategies, pain clinic legislation, clinical guidelines, and naloxone distribution programs, are promising. Evidence of improved health outcomes, particularly from safe storage and disposal strategies and patient education, is weak. Conclusions: While important efforts are underway to affect prescriber and patient behavior, data on state policy and systems-level interventions are limited and inconsistent. Improving the evidence base is a critical need so states, regulatory agencies, and organizations can make informed choices about policies and practices that will improve prescribing and use, while protecting patient health. Published by Elsevier Ireland Ltd. C1 [Haegerich, Tamara M.] Ctr Dis Control & Prevent, Div Unint Injury Prevent, Natl Ctr Injury Prevent & Control, Atlanta, GA 30341 USA. [Paulozzi, Leonard J.] Ctr Dis Control & Prevent, Div Unint Injury Prevent, Natl Ctr Injury Prevent & Control, El Paso, TX 79912 USA. [Manns, Brian J.] Ctr Dis Control & Prevent, Policy Res Anal & Dev Off, Off Associate Director Policy, Atlanta, GA 30029 USA. [Jones, Christopher M.] US PHS, CDR, Off Publ Hlth Strategy & Anal, Off Commissioner,USDA, Silver Spring, MD 20993 USA. RP Haegerich, TM (reprint author), Ctr Dis Control & Prevent, Div Unint Injury Prevent, Natl Ctr Injury Prevent & Control, 4770 Buford Hwy MS F62, Atlanta, GA 30341 USA. EM EQD4@cdc.gov; LBP4@cdc.gov; WMU6@cdc.gov; Christopher.M.Jones@fda.hhs.gov NR 112 TC 57 Z9 57 U1 6 U2 20 PU ELSEVIER IRELAND LTD PI CLARE PA ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND SN 0376-8716 EI 1879-0046 J9 DRUG ALCOHOL DEPEN JI Drug Alcohol Depend. PD DEC 1 PY 2014 VL 145 BP 34 EP 47 DI 10.1016/j.drugalcdep.2014.10.001 PG 14 WC Substance Abuse; Psychiatry SC Substance Abuse; Psychiatry GA AY3XL UT WOS:000347512700002 PM 25454406 ER PT J AU Hudson, LN Newbold, T Contu, S Hill, SLL Lysenko, I De Palma, A Phillips, HRP Senior, RA Bennett, DJ Booth, H Choimes, A Correia, DLP Day, J Echeverria-Londono, S Garon, M Harrison, MLK Ingram, DJ Jung, M Kemp, V Kirkpatrick, L Martin, CD Pan, Y White, HJ Aben, J Abrahamczyk, S Adum, GB Aguilar-Barquero, V Aizen, MA Ancrenaz, M Arbelaez-Cortes, E Armbrecht, I Azhar, B Azpiroz, AB Baeten, L Baldi, A Banks, JE Barlow, J Batary, P Bates, AJ Bayne, EM Beja, P Berg, A Berry, NJ Bicknell, JE Bihn, JH Bohning-Gaese, K Boekhout, T Boutin, C Bouyer, J Brearley, FQ Brito, I Brunet, J Buczkowski, G Buscardo, E Cabra-Garcia, J Calvino-Cancela, M Cameron, SA Cancello, EM Carrijo, TF Carvalho, AL Castro, H Castro-Luna, AA Cerda, R Cerezo, A Chauvat, M Clarke, FM Cleary, DFR Connop, SP D'Aniello, B da Silva, PG Darvill, B Dauber, J Dejean, A Diekotter, T Dominguez-Haydar, Y Dormann, CF Dumont, B Dures, SG Dynesius, M Edenius, L Elek, Z Entling, MH Farwig, N Fayle, TM Felicioli, A Felton, AM Ficetola, GF Filgueiras, BKC Fonte, SJ Fraser, LH Fukuda, D Furlani, D Ganzhorn, JU Garden, JG Gheler-Costa, C Giordani, P Giordano, S Gottschalk, MS Goulson, D Gove, AD Grogan, J Hanley, ME Hanson, T Hashim, NR Hawes, JE Hebert, C Helden, AJ Henden, JA Hernandez, L Herzog, F Higuera-Diaz, D Hilje, B Horgan, FG Horvath, R Hylander, K Isaacs-Cubides, P Ishitani, M Jacobs, CT Jaramillo, VJ Jauker, B Jonsell, M Jung, TS Kapoor, V Kati, V Katovai, E Kessler, M Knop, E Kolb, A Korosi, A Lachat, T Lantschner, V Le Feon, V LeBuhn, G Legare, JP Letcher, SG Littlewood, NA Lopez-Quintero, CA Louhaichi, M Lovei, GL Lucas-Borja, ME Luja, VH Maeto, K Magura, T Mallari, NA Marin-Spiotta, E Marshall, EJP Martinez, E Mayfield, MM Mikusinski, G Milder, JC Miller, JR Morales, CL Muchane, MN Muchane, M Naidoo, R Nakamura, A Naoe, S Nates-Parra, G Navarrete Gutierrez, DA Neuschulz, EL Noreika, N Norfolk, O Noriega, JA Noske, NM O'Dea, N Oduro, W Ofori-Boateng, C Oke, CO Osgathorpe, LM Paritsis, J Parra-H, A Pelegrin, N Peres, CA Persson, AS Petanidou, T Phalan, B Philips, TK Poveda, K Power, EF Presley, SJ Proenca, V Quaranta, M Quintero, C Redpath-Downing, NA Reid, JL Reis, YT Ribeiro, DB Richardson, BA Richardson, MJ Robles, CA Rombke, J Romero-Duque, LP Rosselli, L Rossiter, SJ Roulston, TH Rousseau, L Sadler, JP Safian, S Saldana-Vazquez, RA Samnegard, U Schuepp, C Schweiger, O Sedlock, JL Shahabuddin, G Sheil, D Silva, FAB Slade, EM Smith-Pardo, AH Sodhi, NS Somarriba, EJ Sosa, RA Stout, JC Struebig, MJ Sung, YH Threlfall, CG Tonietto, R Tothmeresz, B Tscharntke, T Turner, EC Tylianakis, JM Vanbergen, AJ Vassilev, K Verboven, HAF Vergara, CH Vergara, PM Verhulst, J Walker, TR Wang, Y Watling, JI Wells, K Williams, CD Willig, MR Woinarski, JCZ Wolf, JHD Woodcock, BA Yu, DW Zaitsev, AS Collen, B Ewers, RM Mace, GM Purves, DW Scharlemann, JPW Purvis, A AF Hudson, Lawrence N. Newbold, Tim Contu, Sara Hill, Samantha L. L. Lysenko, Igor De Palma, Adriana Phillips, Helen R. P. Senior, Rebecca A. Bennett, Dominic J. Booth, Hollie Choimes, Argyrios Correia, David L. P. Day, Julie Echeverria-Londono, Susy Garon, Morgan Harrison, Michelle L. K. Ingram, Daniel J. Jung, Martin Kemp, Victoria Kirkpatrick, Lucinda Martin, Callum D. Pan, Yuan White, Hannah J. Aben, Job Abrahamczyk, Stefan Adum, Gilbert B. Aguilar-Barquero, Virginia Aizen, Marcelo A. Ancrenaz, Marc Arbelaez-Cortes, Enrique Armbrecht, Inge Azhar, Badrul Azpiroz, Adrian B. Baeten, Lander Baldi, Andras Banks, John E. Barlow, Jos Batary, Peter Bates, Adam J. Bayne, Erin M. Beja, Pedro Berg, Ake Berry, Nicholas J. Bicknell, Jake E. Bihn, Jochen H. Boehning-Gaese, Katrin Boekhout, Teun Boutin, Celine Bouyer, Jeremy Brearley, Francis Q. Brito, Isabel Brunet, Joerg Buczkowski, Grzegorz Buscardo, Erika Cabra-Garcia, Jimmy Calvino-Cancela, Maria Cameron, Sydney A. Cancello, Eliana M. Carrijo, Tiago F. Carvalho, Anelena L. Castro, Helena Castro-Luna, Alejandro A. Cerda, Rolando Cerezo, Alexis Chauvat, Matthieu Clarke, Frank M. Cleary, Daniel F. R. Connop, Stuart P. D'Aniello, Biagio da Silva, Pedro Giovani Darvill, Ben Dauber, Jens Dejean, Alain Diekoetter, Tim Dominguez-Haydar, Yamileth Dormann, Carsten F. Dumont, Bertrand Dures, Simon G. Dynesius, Mats Edenius, Lars Elek, Zoltan Entling, Martin H. Farwig, Nina Fayle, Tom M. Felicioli, Antonio Felton, Annika M. Ficetola, Gentile F. Filgueiras, Bruno K. C. Fonte, Steven J. Fraser, Lauchlan H. Fukuda, Daisuke Furlani, Dario Ganzhorn, Joerg U. Garden, Jenni G. Gheler-Costa, Carla Giordani, Paolo Giordano, Simonetta Gottschalk, Marco S. Goulson, Dave Gove, Aaron D. Grogan, James Hanley, Mick E. Hanson, Thor Hashim, Nor R. Hawes, Joseph E. Hebert, Christian Helden, Alvin J. Henden, John-Andre Hernandez, Lionel Herzog, Felix Higuera-Diaz, Diego Hilje, Branko Horgan, Finbarr G. Horvath, Roland Hylander, Kristoffer Isaacs-Cubides, Paola Ishitani, Masahiro Jacobs, Carmen T. Jaramillo, Victor J. Jauker, Birgit Jonsell, Mats Jung, Thomas S. Kapoor, Vena Kati, Vassiliki Katovai, Eric Kessler, Michael Knop, Eva Kolb, Annette Koroesi, Adam Lachat, Thibault Lantschner, Victoria Le Feon, Violette LeBuhn, Gretchen Legare, Jean-Philippe Letcher, Susan G. Littlewood, Nick A. Lopez-Quintero, Carlos A. Louhaichi, Mounir Loevei, Gabor L. Lucas-Borja, Manuel Esteban Luja, Victor H. Maeto, Kaoru Magura, Tibor Mallari, Neil Aldrin Marin-Spiotta, Erika Marshall, E. J. P. Martinez, Eliana Mayfield, Margaret M. Mikusinski, Grzegorz Milder, Jeffrey C. Miller, James R. Morales, Carolina L. Muchane, Mary N. Muchane, Muchai Naidoo, Robin Nakamura, Akihiro Naoe, Shoji Nates-Parra, Guiomar Navarrete Gutierrez, Dario A. Neuschulz, Eike L. Noreika, Norbertas Norfolk, Olivia Noriega, Jorge Ari Noeske, Nicole M. O'Dea, Niall Oduro, William Ofori-Boateng, Caleb Oke, Chris O. Osgathorpe, Lynne M. Paritsis, Juan Parra-H, Alejandro Pelegrin, Nicolas Peres, Carlos A. Persson, Anna S. Petanidou, Theodora Phalan, Ben Philips, T. Keith Poveda, Katja Power, Eileen F. Presley, Steven J. Proenca, Vania Quaranta, Marino Quintero, Carolina Redpath-Downing, Nicola A. Reid, J. Leighton Reis, Yana T. Ribeiro, Danilo B. Richardson, Barbara A. Richardson, Michael J. Robles, Carolina A. Roembke, Joerg Romero-Duque, Luz Piedad Rosselli, Loreta Rossiter, Stephen J. Roulston, T'ai H. Rousseau, Laurent Sadler, Jonathan P. Safian, Szabolcs Saldana-Vazquez, Romeo A. Samnegard, Ulrika Schueepp, Christof Schweiger, Oliver Sedlock, Jodi L. Shahabuddin, Ghazala Sheil, Douglas Silva, Fernando A. B. Slade, Eleanor M. Smith-Pardo, Allan H. Sodhi, Navjot S. Somarriba, Eduardo J. Sosa, Ramon A. Stout, Jane C. Struebig, Matthew J. Sung, Yik-Hei Threlfall, Caragh G. Tonietto, Rebecca Tothmeresz, Bela Tscharntke, Teja Turner, Edgar C. Tylianakis, Jason M. Vanbergen, Adam J. Vassilev, Kiril Verboven, Hans A. F. Vergara, Carlos H. Vergara, Pablo M. Verhulst, Jort Walker, Tony R. Wang, Yanping Watling, James I. Wells, Konstans Williams, Christopher D. Willig, Michael R. Woinarski, John C. Z. Wolf, Jan H. D. Woodcock, Ben A. Yu, Douglas W. Zaitsev, Andrey S. Collen, Ben Ewers, Rob M. Mace, Georgina M. Purves, Drew W. Scharlemann, Joern P. W. Purvis, Andy TI The PREDICTS database: a global database of how local terrestrial biodiversity responds to human impacts SO ECOLOGY AND EVOLUTION LA English DT Article DE Data sharing; global change; habitat destruction; land use ID URBAN-RURAL GRADIENT; LAND-USE CHANGE; INTENSIVELY MANAGED FARMLAND; PLANT COMMUNITY COMPOSITION; MEXICAN COFFEE PLANTATIONS; NORTHEASTERN COSTA-RICA; BOMBUS SPP. HYMENOPTERA; DUNG BEETLE COLEOPTERA; BUMBLEBEE NEST DENSITY; BIRD SPECIES RICHNESS AB Biodiversity continues to decline in the face of increasing anthropogenic pressures such as habitat destruction, exploitation, pollution and introduction of alien species. Existing global databases of species' threat status or population time series are dominated by charismatic species. The collation of datasets with broad taxonomic and biogeographic extents, and that support computation of a range of biodiversity indicators, is necessary to enable better understanding of historical declines and to project - and avert - future declines. We describe and assess a new database of more than 1.6 million samples from 78 countries representing over 28,000 species, collated from existing spatial comparisons of local-scale biodiversity exposed to different intensities and types of anthropogenic pressures, from terrestrial sites around the world. The database contains measurements taken in 208 (of 814) ecoregions, 13 (of 14) biomes, 25 (of 35) biodiversity hotspots and 16 (of 17) megadiverse countries. The database contains more than 1% of the total number of all species described, and more than 1% of the described species within many taxonomic groups - including flowering plants, gymnosperms, birds, mammals, reptiles, amphibians, beetles, lepidopterans and hymenopterans. The dataset, which is still being added to, is therefore already considerably larger and more representative than those used by previous quantitative models of biodiversity trends and responses. The database is being assembled as part of the PREDICTS project (Projecting Responses of Ecological Diversity In Changing Terrestrial Systems - ). We make site-level summary data available alongside this article. The full database will be publicly available in 2015. C1 [Hudson, Lawrence N.; Contu, Sara; Hill, Samantha L. L.; De Palma, Adriana; Phillips, Helen R. P.; Choimes, Argyrios; Correia, David L. P.; Echeverria-Londono, Susy; Purvis, Andy] Nat Hist Museum, Dept Life Sci, London SW7 5BD, England. [Newbold, Tim; Hill, Samantha L. L.; Senior, Rebecca A.; Booth, Hollie; Scharlemann, Joern P. W.] World Conservat Monitoring Ctr, United Nations Environm Programme, Cambridge CB3 0DL, England. [Newbold, Tim; Purves, Drew W.] Microsoft Res, Computat Ecol & Environm Sci, Cambridge CB1 2FB, England. [Lysenko, Igor; De Palma, Adriana; Phillips, Helen R. P.; Bennett, Dominic J.; Choimes, Argyrios; Day, Julie; Echeverria-Londono, Susy; Garon, Morgan; Harrison, Michelle L. K.; Kemp, Victoria; Martin, Callum D.; Dures, Simon G.; Fayle, Tom M.; Tylianakis, Jason M.; Ewers, Rob M.; Purvis, Andy] Imperial Coll London, Ascot SL5 7PY, Berks, England. [Booth, Hollie] Frankfurt Zool Soc, Africa Reg Off, Arusha, Tanzania. [Ingram, Daniel J.; Goulson, Dave; Scharlemann, Joern P. W.] Univ Sussex, Sch Life Sci, Brighton BN1 9QG, E Sussex, England. [Jung, Martin] Nat Hist Museum Denmark, Ctr Macroecol Climate & Evolut, DK-2100 Copenhagen, Denmark. [Kirkpatrick, Lucinda] Univ Stirling, Sch Biol & Ecol Sci, Stirling FK9 4LA, Scotland. [Pan, Yuan] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England. [White, Hannah J.] Queens Univ Belfast, Sch Biol Sci, Belfast BT9 7BL, Antrim, North Ireland. [Aben, Job] Univ Antwerp, Evolutionary Ecol Grp, B-2020 Antwerp, Belgium. [Abrahamczyk, Stefan] Univ Bonn, Nees Inst Plant Biodivers, D-53113 Bonn, Germany. [Adum, Gilbert B.; Oduro, William] KNUST, CANR, FRNR, Dept Wildlife & Range Management, Kumasi, Ghana. [Adum, Gilbert B.; Oduro, William] SAVE FROGS Ghana, Adum Kumasi, Ghana. [Aguilar-Barquero, Virginia] Univ Costa Rica, Escuela Biol, San Jose 2060, Costa Rica. [Aizen, Marcelo A.; Morales, Carolina L.] Univ Nacl Comahue, CONICET, Lab INIBIOMA, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. [Ancrenaz, Marc] HUTAN Kinabatangan Orang Utan Conservat Programme, Kota Kinabalu 88874, Sabah, Malaysia. [Arbelaez-Cortes, Enrique] Univ Nacl Autonoma Mexico, Fac Ciencias, Museo Zool, Mexico City 04510, DF, Mexico. [Arbelaez-Cortes, Enrique] Inst Invest Recursos Biol Alexander von Humboldt, Colecc Tejidos, Valle Del Cauca, Colombia. [Armbrecht, Inge] Univ Valle, Dept Biol, Cali, Colombia. [Azhar, Badrul] Univ Putra Malaysia, Inst Biosci, Biodivers Unit, Serdang 43400, Selangor, Malaysia. [Azhar, Badrul] Univ Putra Malaysia, Fac Forestry, Serdang 43400, Selangor, Malaysia. [Azpiroz, Adrian B.] Inst Invest Biol Clemente Estable, Lab Genet Conservac, Montevideo, Uruguay. [Baeten, Lander] Univ Ghent, Dept Forest & Water Management, Forest & Nature Lab, B-9090 Gontrode, Belgium. [Baeten, Lander] Univ Ghent, Dept Biol, Terr Ecol Unit, B-9000 Ghent, Belgium. [Baldi, Andras] MTA Ctr Ecol Res, H-2163 Vacratot, Hungary. [Banks, John E.] Univ Washington, Tacoma, WA 98402 USA. [Barlow, Jos] Univ Lancaster, Lancaster Environm Ctr, Lancaster LA1 4YQ, England. [Barlow, Jos] MCT Museu Paraense Emilio Goeldi, Belem, Para, Brazil. [Batary, Peter; Tscharntke, Teja] Univ Gottingen, D-37077 Gottingen, Germany. [Bates, Adam J.] Univ Birmingham, Birmingham B15 2TT, W Midlands, England. [Bayne, Erin M.] Univ Alberta, Dept Biol Sci, CW Biol Sci Ctr 405, Edmonton, AB T6G 2E9, Canada. [Beja, Pedro] Univ Porto, Ctr Invest Biodiversidade & Recursos Genet, EDP Biodivers Chair, CIBIO InBio, P-4485601 Vairao, Portugal. [Berg, Ake] Swedish Univ Agr Sci, Swedish Biodivers Ctr, SE-75007 Uppsala, Sweden. [Berry, Nicholas J.] Univ Edinburgh, Sch GeoSci, Edinburgh EH9 3JN, Midlothian, Scotland. [Bicknell, Jake E.; Struebig, Matthew J.] Univ Kent, DICE, Sch Anthropol & Conservat, Canterbury CT2 7NR, Kent, England. [Bicknell, Jake E.] Iwokrama Int Ctr Rainforest Conservat & Dev, Georgetown, Guyana. [Bihn, Jochen H.] Univ Marburg, Dept Anim Ecol, D-35032 Marburg, Germany. [Boehning-Gaese, Katrin; Neuschulz, Eike L.; Roembke, Joerg] Senckenberg Gesell Naturforsch, Biodivers & Climate Res Ctr BiK F, D-60325 Frankfurt, Germany. [Boehning-Gaese, Katrin] Goethe Univ Frankfurt, Inst Ecol Evolut & Divers, D-60439 Frankfurt, Germany. [Boekhout, Teun] CBS KNAW Fungal Biodivers Ctr, Utrecht, Netherlands. [Boutin, Celine] Carleton Univ, Sci & Technol Branch, Environm Canada, Ottawa, ON K1A 0H3, Canada. [Bouyer, Jeremy] Ctr Cooperat Int Rech Agron Dev CIRAD, Unite Mixte Rech Controle Malad Anim Exot & Emerg, F-34398 Montpellier, France. [Bouyer, Jeremy] INRA, Unite Mixte Rech Controle Malad Anim Exot & Emerg, F-34398 Montpellier, France. [Brearley, Francis Q.] Manchester Metropolitan Univ, Sch Sci & Environm, Manchester M1 5GD, Lancs, England. [Brito, Isabel] Univ Evora ICAAMA, P-7002554 Evora, Portugal. [Brunet, Joerg] Swedish Univ Agr Sci, Southern Swedish Forest Res Ctr, S-23053 Alnarp, Sweden. [Buczkowski, Grzegorz] Purdue Univ, Dept Entomol, W Lafayette, IN 47907 USA. [Buscardo, Erika] Univ Coimbra, Dept Ciencias Vida, Ctr Ecol Func, P-3000456 Coimbra, Portugal. [Buscardo, Erika] Inst Nacl de Pesquisas da Amazonia, Escritorio Cent LBA, BR-69060001 Manaus, AM, Brazil. [Buscardo, Erika] Univ Dublin Trinity Coll, Sch Nat Sci, Dept Bot, Dublin 2, Ireland. [Cabra-Garcia, Jimmy] Univ Sao Paulo, Inst Biociencias, Dept Zool, BR-05508090 Sao Paulo, SP, Brazil. [Calvino-Cancela, Maria] Univ Vigo, Dept Ecol & Anim Biol, Fac Sci, Vigo 36310, Spain. [Cameron, Sydney A.] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA. [Cancello, Eliana M.; Carrijo, Tiago F.] Univ Sao Paulo, Museu Zool, BR-04263000 Sao Paulo, SP, Brazil. [Carvalho, Anelena L.] Inst Nacl de Pesquisas da Amazonia, BR-69067375 Manaus, AM, Brazil. [Castro, Helena] Univ Coimbra, Dept Life Sci, Ctr Funct Ecol, P-3000456 Coimbra, Portugal. [Castro-Luna, Alejandro A.] Univ Veracruzana, Inst Biotecnol & Ecol Aplicada INBIOTECA, Xalapa 91090, Veracruz, Mexico. [Cerda, Rolando; Somarriba, Eduardo J.] Trop Agr Res & Higher Educ Ctr, CATIE, Turrialba 30501, Costa Rica. [Cerezo, Alexis] Univ Buenos Aires, Fac Agron, Dept Quantitat Methods & Informat Syst, RA-1417 Buenos Aires, DF, Argentina. [Chauvat, Matthieu] Normandie Univ, EA ECODIV Rouen 1293, SFR SCALE, UFR Sci & Tech, F-76821 Mont St Aignan, France. [Clarke, Frank M.] Univ Aberdeen, Aberdeen AB24 2TZ, Scotland. [Cleary, Daniel F. R.] Univ Aveiro, CESAM, Dept Biol, P-3810193 Aveiro, Portugal. [Connop, Stuart P.] Univ London, Sustainabil Res Inst, London E16 2RD, England. [D'Aniello, Biagio] Univ Naples Federico II, Dept Biol, Naples, Italy. [da Silva, Pedro Giovani] Univ Fed Santa Catarina, Programa Posgrad Ecol, BR-88040900 Florianopolis, SC, Brazil. [Darvill, Ben] Univ Stirling, British Trust Ornithol, Stirling FK9 4LA, Scotland. [Dauber, Jens] Thunen Inst Biodivers, D-38116 Braunschweig, Germany. [Dejean, Alain] Ecol Forets Guyane UMR CNRS 8172, CNRS, F-97379 Kourou, France. [Dejean, Alain] Univ Toulouse, Lab Ecol Fonct & Environm Ecolab, UPS, INP, F-31062 Toulouse, France. [Diekoetter, Tim] Univ Kiel, Inst Nat & Resource Conservat, Dept Landscape Ecol, D-24098 Kiel, Germany. [Diekoetter, Tim] Univ Marburg, Dept Biol, Nat Conservat, Marburg, Germany. [Diekoetter, Tim] Swiss Fed Inst Technol, Inst Integrat Biol, Zurich, Switzerland. [Dominguez-Haydar, Yamileth] Univ Atlantico, Programa Biol, Atlantico, Colombia. [Dormann, Carsten F.] Univ Freiburg, D-79106 Freiburg, Germany. [Dumont, Bertrand] INRA, Herbivores UMR1213, F-63122 St Genes Champanelle, France. [Dures, Simon G.] Zool Soc London, Inst Zool, London NW1 4RY, England. [Dynesius, Mats] Umea Univ, Dept Ecol & Environm Sci, S-90187 Umea, Sweden. [Edenius, Lars] Swedish Univ Agr Sci, S-90183 Umea, Sweden. [Elek, Zoltan] Eotvos Lorand Univ, Hungarian Acad Sci, Inst Biol, MTA ELTE MTM Ecol Res Grp, H-1117 Budapest, Hungary. [Entling, Martin H.] Univ Koblenz Landau, Inst Environm Sci, D-76829 Landau, Germany. [Farwig, Nina] Univ Marburg, Dept Ecol Conservat Ecol, Fac Biol, D-35032 Marburg, Germany. [Fayle, Tom M.] Univ South Bohemia, Fac Sci, Ceske Budejovice 37005, Czech Republic. [Fayle, Tom M.] Univ Malaysia Sabah, Inst Trop Biol & Conservat, Kota Kinabalu 88999, Sabah, Malaysia. [Felicioli, Antonio] Univ Pisa, Dipartimento Sci Vet, I-56124 Pisa, Italy. [Felton, Annika M.] Swedish Univ Agr Sci, Southern Swedish Forest Res Ctr, S-23453 Alnarp, Sweden. [Ficetola, Gentile F.] Univ Grenoble Alpes, Lab Ecol Alpine LECA, F-38000 Grenoble, France. [Filgueiras, Bruno K. C.] Univ Fed Pernambuco, Programa Posgrad Biol Anim, BR-50670901 Recife, PE, Brazil. [Fonte, Steven J.] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA. [Fraser, Lauchlan H.] Thompson Rivers Univ, Dept Nat Resource Sci, Kamloops, BC V2C 0C8, Canada. [Fukuda, Daisuke] IDEA Consultants Inc, Okinawa Branch Off, Naha, Okinawa 9000003, Japan. [Furlani, Dario] Carl Zeiss Microscopy GmbH, D-37081 Gottingen, Germany. [Ganzhorn, Joerg U.] Univ Hamburg, Bioctr Grindel, D-20146 Hamburg, Germany. [Garden, Jenni G.] Seed Consulting Serv, Adelaide, SA 5000, Australia. [Garden, Jenni G.] Univ Queensland, Sch Geog Planning & Environm Management, St Lucia, Qld 4072, Australia. [Gheler-Costa, Carla] USC, Sao Paulo, Brazil. [Giordani, Paolo] Univ Genoa, DISTAV, I-16136 Genoa, Italy. [Giordano, Simonetta] Univ Naples Federico II, Dipartimento Biol, I-80126 Naples, Italy. [Gottschalk, Marco S.] Univ Fed Pelotas UFPel, BR-96010900 Pelotas, RS, Brazil. [Gove, Aaron D.] Astron Environm Serv, East Perth, WA 6004, Australia. [Gove, Aaron D.] Curtin Univ, Dept Environm & Agr, Bentley, WA 6102, Australia. [Grogan, James] Mt Holyoke Coll, Dept Biol Sci, S Hadley, MA 01075 USA. [Hanley, Mick E.] Univ Plymouth, Sch Biol Sci, Plymouth PL4 8AA, Devon, England. [Hashim, Nor R.] Int Univ Malaya Wales, Kuala Lumpur 50480, Malaysia. [Hawes, Joseph E.] Museu Paraense Emilio Goeldi, Coordenacao Bot, BR-66040170 Belem, Para, Brazil. [Hawes, Joseph E.] Univ E Anglia, Sch Environm Sci, Norwich NR4 7TJ, Norfolk, England. [Hebert, Christian] Nat Resources Canada, Canadian Forest Serv, Laurentian Forestry Ctr, Quebec City, PQ G1V 4C7, Canada. [Helden, Alvin J.] Anglia Ruskin Univ, Dept Life Sci, Anim & Environm Res Grp, Cambridge CB1 1PT, England. [Henden, John-Andre] Univ Tromso, Dept Arctic & Marine Biol, N-9037 Tromso, Norway. [Hernandez, Lionel] Univ Nacl Expt Guayana, Puerto Ordaz 8015, Estado Bolivar, Venezuela. [Herzog, Felix] Agroscope, CH-8046 Zurich, Switzerland. [Higuera-Diaz, Diego] Corp Sentido Nat, Bogota, Colombia. [Hilje, Branko] Tecnol Costa Rica, Escuela Ingn Forestal, Cartago, Costa Rica. [Hilje, Branko] Asociac Conservac Estudio Biodiversidad ACEBIO, San Jose, Costa Rica. [Horgan, Finbarr G.] Int Rice Res Inst, Manila, Philippines. [Horvath, Roland] Univ Debrecen, Dept Ecol, H-4010 Debrecen, Hungary. [Hylander, Kristoffer] Stockholm Univ, Dept Ecol Environm & Plant Sci, S-10691 Stockholm, Sweden. [Isaacs-Cubides, Paola] Inst Invest & Recursos Biol Alexander von Humbold, Bogota, Colombia. [Ishitani, Masahiro] Hiroshima Univ, Grad Sch Educ, Higashihiroshima 7398524, Japan. [Jacobs, Carmen T.] Univ Pretoria, Scarab Res Grp, ZA-0002 Pretoria, South Africa. [Jaramillo, Victor J.] Univ Nacl Autonoma Mexico, Ctr Invest Ecosistemas, Morelia 58090, Michoacan, Mexico. [Jauker, Birgit] Univ Giessen, Dept Anim Ecol, D-35392 Giessen, Germany. [Jonsell, Mats] Swedish Univ Agr Sci, Dept Ecol, S-75007 Uppsala, Sweden. [Jung, Thomas S.] Yukon Dept Environm, Whitehorse, YT Y1A 2C6, Canada. [Kapoor, Vena] Nat Conservat Fdn, Mysore, Karnataka, India. [Kati, Vassiliki] Univ Patras, Dept Environm & Nat Resources Management, Agrinion 30100, Greece. [Katovai, Eric] James Cook Univ, Ctr Trop Environm & Sustainabil Sci TESS, Cairns, Qld, Australia. [Katovai, Eric] Pacific Adventist Univ, Sch Sci & Technol, Port Moresby, Papua N Guinea. [Kessler, Michael] Univ Zurich, Inst Systemat Bot, CH-8008 Zurich, Switzerland. [Knop, Eva; Schueepp, Christof] Univ Bern, Inst Ecol & Evolut, CH-3012 Bern, Switzerland. [Kolb, Annette] Univ Bremen, Inst Ecol, FB2, D-28359 Bremen, Germany. [Koroesi, Adam] MTA ELTE MTM Ecol Res Grp, H-1117 Budapest, Hungary. [Koroesi, Adam] Univ Wurzburg, Field Stn Fabrikschleichach, Bioctr, D-96181 Rauhenebrach, Germany. [Lachat, Thibault] Swiss Fed Inst Forest Snow & Landscape Res WSL, CH-8903 Birmensdorf, Switzerland. [Lantschner, Victoria] EEA Bariloche, Inst Nacl Tecnol Agr, RA-8400 San Carlos De Bariloche, Rio Negro, Argentina. [Le Feon, Violette] INRA, UR Abeilles & Environm 406, F-84914 Avignon, France. [LeBuhn, Gretchen] San Francisco State Univ, Dept Biol, San Francisco, CA 94132 USA. [Legare, Jean-Philippe] Minist A